bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extended-spectrum of KRAS and NRAS mutations in lung cancer tissue specimens obtained with bronchoscopy
Authors: Basdemirci Muserref , Zamani Adil , Zamani Ayse G. , Findik Siddika , Yildirim Mahmut S 
Published: June 2021

Background:
Mutations in the RAS genes, HRAS, KRAS, and NRAS, are the most common modifications in many types of human tumors and are found in approximately 30% of all human cancers. These mutations are usually found in codons 12, 13, or 61.

Methods:
The aim of this study is to evaluate mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12,13, and 61 of KRAS gene in lung cancer tissue specimens obtained with bronchoscopy. KRAS and NRAS mutation analyses with pyrosequencing were performed on DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue samples of 64 patients histopathologically diagnosed as lung cancer after bronchoscopic biopsy.

Results:
In all, 20 patients (31.2%) had mutations in KRAS gene (8/27 squamous cell carcinoma, 8/11 adenocarcinoma, 3/16 small cell carcinoma, and 1/1 pleomorphic carcinoma). The most common mutation in codon 12 was in c.35G>T (G12V). When the mutation rate of adenocarcinoma (72.7%) and squamous cell carcinoma (22.9%) patients was compared with each other, a statistically significant difference was observed (P = 0.008). There were no mutations in codons 59, 117, or 146 of KRAS and NRAS genes in patients with lung cancer.

Conclusion:
In this study, we firstly examined mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12, 13, and 61 of KRAS gene in Turkish lung cancer patients both in non-small cell lung cancer and small cell lung cancer. Although no mutation was detected in codons 59, 117, and 146 of KRAS and NRAS genes, the frequency of KRAS gene mutation was higher than the rate of mutation in both Asian and Western countries, and multicenter studies including more cases should be performed to further explore our results.
Factors influencing women to participate in cervical cancer screening by providing menstrual pads
Authors: Budukh Atul , Maheshwari Amita , Bagal Sonali , Singh Arpit , Deodhar Kedar , Panse Nandkumar , Palyekar Vrushali , Dikshit Rajesh , Badwe Rajendra 
Published: June 2021

Background:
India accounts for a quarter of the world cervical cancer burden. Cervical cancer is highly preventable. However, low level of participating women in screening is one of the major issues. The aim of this work was to study the factors that influence women to participate in cervical cancer screening by providing menstrual pads for human papillomavirus (HPV) testing.

Methods:
Menstrual clothes were collected from two different populations from the rural areas of Maharashtra state for HPV testing to screen for cervical cancer. For this study, out of 945 participated women, 557 (58.9%) provided their menstrual pads. Multivariate logistic regression was applied to calculate the odds ratio (OR) and 95% confidence interval (95% CI).

Results:
The probability of providing the menstrual pads was high among the women who were highly educated compared to those with less education (OR: 1.4; 95% CI: 1.0–1.9), having mobile phone facilities as compared to those with no mobile phones (OR: 1.4; 95% CI: 1.0–2.0), who were using new cloths as menstrual pads compared to those who did not use the same (OR: 8.5; 95% CI: 5.0–14.3), who did not have tobacco habit as compared to those who had tobacco habit (OR: 1.4; 95% CI: 1.1–1.9) and in the village where health worker was stationed as compared to the village where health worker was not stationed (OR: 1.8; 95% CI: 1.4–2.5).

Conclusion:
Factors including health worker availability, using mobile phones for communication and high education level facilitate women’s participation. To improve the participation, there is need to apply special strategies for older age group, less educated women and women having tobacco habit.
Rapid detection of Mycobacterium tuberculosis in pus samples of suspected cases of extrapulmonary tuberculosis by GeneXpert MTB/RIF assay and its comparison with conventional methods
Authors: Kumar Rakesh , Muni Sweta , Pankaj Deepak , Kumar Randhir , Saurabh Kumar , Kumar Shailesh , Kumari Namrata , Shahi Shivendra Kumar 
Published: June 2021

Introduction:
GeneXpert MTB/RIF or cartridge-based nucleic acid amplification test is a rapid diagnostic tool used for the detection of Mycobacterium tuberculosis and its resistance to rifampicin. It can be offered as a first-line diagnostic modality in suspected cases of extrapulmonary tuberculosis (TB). This study was done to evaluate the diagnostic accuracy of the Xpert MTB/RIF assay for the detection of M. tuberculosis in extrapulmonary specimens and its resistance to rifampicin.

Materials and Methods:
This study was done in the Department of Microbiology at Indira Gandhi Institute of Medical Sciences, Patna, Bihar, over a period 18 months from January 2018 to June 2019 in 508 patients. The analysis and interpretation of the data were performed using Microsoft excel. The quantitative data obtained were expressed as percentage in tabular form.

Results:
Out of a total of 508 pus samples in suspected extrapulmonary TB patients, the percentage of males was more than females with male-to-female ratio being 1.6:1. Among the testing of Mycobacterium tuberculosis by various methods, maximum detection was done by Xpert MTB/RIF assay in 37% of cases. The least number of cases were detected by Ziehl–Neelsen staining (8.1%). Rifampicin resistance was detected in 14.9% of cases (28/188) while its resistance was not detected in 85.1% of cases (160/188) among cases of M. tuberculosis detected. Many of the negative samples on fluorescent and Ziehl–Neelsen staining came to be positive with GeneXpert testing assuring the sensitivity and specificity of Xpert MTB/RIF assay.

Conclusion:
In today's era when we are going for TB elimination, not only rapid TB case detection but also the early determination of multidrug resistance status is of prime value. This will not only assist in the identification of the patient with disease and drug resistance but also initiate prompt early intervention and treatment and moving a step closer in achieving the goal of TB elimination.
Study of seroconversion for severe acute respiratory syndrome
Authors: Kumari Rekha , Kumari Sweta , Kumar Santosh , Bharti Neha 
Published: June 2021

Background:
SARS-CoV-2 possess higher risk of infection to Health Care Worker (HCW) from patients or other fellow HCW.

Aims and Objective:
The aim of this study is to estimate the seroprevalence of SARS-CoV-2 Ig G antibody in a sample of HCW chosen randomly from a tertiary care hospital in Patna Bihar.

Results:
Of the 169 participants screened from 8 July to 26 August 2020, 10.1%, (95% CI: 5.97-15.62) were seropositive for IgG antibody against SARS-CoV-2. The cumulative prevalence of SARS-CoV-2 infection (presence of antibodies or past or current positive rRT-PCR) was 10.7% (95% CI: 6.4–16.3). Among those with evidence of past or current infection, 5.6 % (1/18) had not been previously diagnosed with COVID-19.

Conclusion:
We have observed a relatively low seroprevalence of antibodies among HCW at the peak of the COVID-19 epidemic in IGIMS Patna. Seroconversion occur with a mean of 14 days in HCW from the day of diagnosis with past or present infection of COVID-19.
Permasalahan dan Alternatif Strategi Kebijakan dalam Upaya Penurunan Angka Kematian Ibu (Studi di Kabupaten Pasaman Barat, Provinsi SumateraBarat)
Authors: Media Yulfira 
Published: June 2021

Angka Kematian Ibu (AKI) masih menjadi masalah besar dan perlu mendapatkan perhatian yang serius di Indonesia dan termasuk juga di Provinsi Sumatera Barat. Tujuan penelitian adalah menggambarkan permasalahan dalam penurunan AKI dan merumuskan alternatif strategi kebijakan dalam upaya penurunannya. Penelitian dengan menggunakan metode penelitian kualitatif dengan teknik pengumpulan data melalui wawancara mendalam dan observasi. Hasil penelitian mengungkapkan bahwa beberapa permasalahan dalam upaya penurunan AKI adalah belum memadainya ketersediaan akses pelayanan kesehatan ibu dan anak, dan permasalahan sosial budaya dan ekonomi masyarakat dalam pemanfaatan layanan kesehatan ibu dan anak. Dalam upaya penurunan AKI ada beberapa alternatif strategi kebijakan yaitu strategi peningkatan ketersediaan dan mutu penanganan kesehatan ibu di fasilitas pelayanan kesehatan serta penguatan rujukan persalinan, penguatan kapasitas sumber daya kesehatan yang mempunyai kompetensi, peningkatan cakupan pelayanan kesehatan ibu yang lebih menitikberatkan promotif dan preventif dan optimalisasi pemberdayaan masyarakat.
ANAIS DO I CONGRESSO INTERNACIONAL DE MEDICINA DO SUDOESTE DO PARANÁ
Authors: INTERNACIONAL DE MEDICINA DO SUDOESTE DO PARANÁ ANAIS DO I CONGRESSO 
Published: June 2021

Comissão Científica I Congresso Internacional Médico do Sudoeste do Paraná Carolina PanisEmanuela Kerkhoff UtzigFernanda Mara AlvesGéssica Tuani TeixeiraJanaína Carla da SilvaJulia Fratin da SilvaMaria Eduarda Fontana VasselaiMariane Okamoto FerreiraNathally Ghilardi CardosoRayana Turra Damo Rodrigo KernStefania Tagliari de Oliveira
INTERNETÊS VERSUS ESCRITA FORMAL: A NOVA ESCRITA E SEUS DESDOBRAMENTOS
Authors: Gallardo Barbara Cristina , Kobayashi Eliana 
Published: June 2021

Com o advento da internet, o acesso ao meio digital proporcionou novas formas de comunicação, dentre elas, a escrita com características de fala, em um contexto mais abrangente do que o meio físico, além de um acesso mais abrangente à informação. Com isso, as práticas de leitura e escrita mudaram significativamente, promovendo outros olhares para as inadequações gramaticais e o surgimento de uma escrita própria da internet: o internetês. Neste trabalho, analisamos a reescrita de 4 posts publicados em uma rede social online, realizadas por estudantes do 1º. ano do ensino médio de duas escolas, uma pública e uma privada. O objetivo foi verificar i) a influência da escrita digital no conhecimento da norma padrão dos participantes; ii) semelhanças e discrepâncias entre os resultados dos dois grupos; iii) o equilíbrio entre os grupos, em relação à consciência dos gêneros textuais e a questões de situacionalidade. A metodologia empregada seguiu os preceitos do estudo de caso, tendo a pesquisa bibliográfica sobre o assunto como embasamento. É um estudo exploratório de natureza quanti-qualitativa, cujo instrumento de coleta foi um handout com a reprodução dos posts retirados de uma rede social e entregues aos estudantes para a leitura, identificação e reescrita de inadequações, próprias da escrita virtual. A fundamentação teórica foi ancorada, principalmente, nas discussões de Freitag e Fonseca e Silva (2006) e Mollica e Batista (2015) sobre as inadequações gramaticais; Komesu e Tanani (2009) sobre o internetês; Marcuschi e Dionísio (2007) sobre a relação entre fala e escrita. Os resultados chamam a atenção para a necessidade da construção de práticas curriculares que contribuam para a conscientização de estudantes sobre o conhecimento de diferentes gêneros textuais, tendo em vista as práticas de leitura e escrita que participam na internet.
MENU GIZI SEIMBANG DAN PEMERIKSAAN KESEHATAN PADA BALITA DI POSKESDES RIA-RIA KECAMATAN POLLUNG KABUPATEN HUMBANG HASUNDUTAN
Authors: Fransiska Fransiska , Mayes Mayes 
Published: June 2021

Menu makanaan seimbang merupakan susunan makanan yang dimakan sehari hari untuk mencapai kecukupan gizi dalam tubuh yang terdiri dari beranekaragam makanan dalam jumlah dan proporsi yang sesuai, sehingga memenuhi kebutuhan gizi seseorang guna pemeliharaan dan perbaikan sel-sel tubuh dan proses kehidupan serta pertumbuhan dan perkembangan. Pada masa balita hal ini menu seimbang diperlukan untuk periode perkembangan fisik dan mental yang pesat. Pada masa ini, otak balita telah siap menghadapi berbagai stimulasi seperti belajar berjalan dan berbicara lebih lancar. Metode pengabdian masyarakat ini dengan cara kualitatif dan kuantitatif, wawancara dan observasi ke rumah rumah penduduk. Hasil pengabdian masyarakat menunjukkan masyarakat mulai memahami penting nya menu seimbang dan melakukan pemeriksaan kesehatan pada anak balitanya. Diharapkan masyarakat secara kontiniu melakukan pemeriksaan kesehatan pada balita dan mempertahankan asupan meni seimbang pada balita nya.
DIFICULDADES DE APRENDIZAGEM E A PANDEMIA: AGRAVAMENTO OU EVIDENCIAMENTO DA DIFICULDADE JÁ EXISTENTE?
Authors: Oliveira Thamires Maia Paula , Carvalho Karollainy Gonçalves Coelho 
Published: June 2021

A presente reflexão tem a intencionalidade de demonstrar os evidenciamentos e agravamentos causados pela pandemia nas dificuldades de aprendizagem do ensino fundamental. Sabe-se que após a inserção da tecnologia na vida cotidiana, as crianças têm se dispersado com mais facilidade, apresentado ansiedade e falta de interesse nas exposições tradicionais das atividades escolares. No entanto, a análise mostra que a pandemia apenas elevou o estágio em que esse indivíduo já se encontrava, as aulas remotas deixaram as casas e famílias mais vulneráveis e suscetíveis as observações técnicas dos professores. Constatamos então, que as crianças que já eram estimuladas e assistidas pelos pais, não apresentaram fartas dificuldades de adaptação e as mesmas tiveram o desempenho esperado para a faixa etária, enquanto as crianças que tiveram menores desempenhos e crescente dificuldades expostas, tratam-se de crianças com baixa autoestima, baixa autonomia e desprovidas de apoio e assistência familiar.
Becoming Patriots: The Struggle for Inclusion and Exclusion on Pennsylvania’s Revolutionary Frontier
Authors: Pearl Chris 
Published: June 2021

Abstract
This article explores the American Revolution on the Pennsylvania frontier by examining the creation of an extralegal government of “Fair Play” squatters on Native American land near the northwest branch of the Susquehanna River. It also studies their efforts to legitimize themselves and their claims through attachment to the “Common Cause.” The motivation of squatters to mobilize support for the American Revolution was directly related to a prolonged conflict between themselves, Native Americans, speculators, and colonial and imperial officials over land and power. Moreover, “Fair Play” squatters used their dedication to the Revolutionary War to cast the defense of their illegal claims, the extralegal government they created, and their violence against Native Americans as a patriotic duty. This proved a successful tactic to include their community and its ideals into an emerging republican state.
Algemene voorwaarden en dienstverrichters, onduidelijk gepuzzel: wie het weet mag het zeggen
Authors: Van Wechem T.H.M. , Spanjaard J.H.M. 
Published: June 2021

Algemene voorwaarden en dienstverrichters, onduidelijk gepuzzel: wie het weet mag het zeggen
De Dienstenrichtlijn verplicht de dienstverrichter tot het verstrekken van uitgebreide informatie aan de klant, waaronder algemene voorwaarden. De Dienstenrichtlijn bevat geen sancties op het verzaken van de informatieplicht. De Nederlandse wetgever heeft de informatieplicht gekoppeld aan artikel 6:234 BW. Auteurs onderzoeken of dat juist is en of het verzaken van de informatieplicht een totstandkomingsgebrek is.
Invisible before the law
Authors: Schuthof F. 
Published: June 2021

Invisible before the law The legal position of persons with intellectual disabilities under the Dutch Care and Compulsion Act (Wzd) in light of Article 12 of the Convention on the Rights of Persons with Disabilities (CRPD)
In the Netherlands, the use of involuntary treatment in the mental health care sector is governed by the Dutch Care and Compulsion Act (Wzd). This study examines the legal position of persons with intellectual disabilities under this Act. The Wzd is analyzed in light of the human rights standards of Article 12 of the Convention on the Rights of Persons with Disabilities (CRPD). The findings of this study show that the Wzd does not meet the standards of Article 12 in several cases. The Wzd does not recognize the legal capacity of persons with intellectual disabilities, it continues to allow for substituted decision-making and support measures are not complemented by adequate safeguards. From a theoretical point of view, an imbalance between the protection of and the respect for the autonomy of persons with intellectual disabilities can be observed. This article formulates several recommendations in order to restore this balance.---De Nederlandse Wet zorg en dwang (Wzd) ziet toe op de rechten van mensen met een verstandelijke beperking bij onvrijwillige zorg of onvrijwillige opname. Dit artikel onderzoekt de juridische positie van mensen met een verstandelijke beperking ten aanzien van deze wet. De Wzd wordt geanalyseerd in relatie tot artikel 12 van het Verdrag inzake de Rechten van Personen met een Handicap (VRPH). De bevindingen van dit onderzoek laten zien dat de Wzd in verschillende gevallen niet voldoet aan de normen van artikel 12 VRPH. Zo wordt onder andere de handelingsbekwaamheid, ofwel ‘legal capacity’, van mensen met een verstandelijke beperking niet erkend en blijft plaatsvervangende besluitvorming mogelijk. Vanuit theoretisch oogpunt is er sprake van een disbalans tussen de bescherming van en het respect voor de autonomie van mensen met een verstandelijke beperking. Dit artikel doet daarom meerdere aanbevelingen om dit evenwicht te herstellen.
The Early Pleistocene ectothermic vertebrates of Pietrafitta (Italy) and the last Western European occurrence of Latonia Meyer, 1843
Authors: SORBELLI Leonardo , Andrea VILLA undefined , Sergio GENTILI undefined , Marco CHERIN undefined , Giorgio CARNEVALE undefined , Emanuel TSCHOPP undefined , Massimo DELFINO undefined 
Published: June 2021

The Early Pleistocene site of Pietrafitta (central Italy) produced a rich vertebrate assemblage from the Late Villafranchian Land Mammal Age (late MN18). Geological and paleobotanical data from Pietrafitta indicate a lacustrine environment, surrounded by a humid deciduous broadleaved forest with a temperate climate. The vertebrate assemblage consists of at least 40 taxa including actinopterygians, amphibians, reptiles, birds, and mammals. Here, we concentrate on the ichthyofaunal and herpetofaunal remains. The ichthyofauna includes Barbus Cuvier and Cloquet, 1816, Scardinius Bonaparte, 1837, aff. Squalius Cuvier, 1817, and Tinca Cuvier, 1817. The two anuran genera are the large-sized alytid frog Latonia Meyer, 1843 and the “green frog” Pelophylax Fitzinger, 1843. Three snake precloacal vertebrae were recognized, one attributed to Colubrines indet., another one to ­Natrix sp. Laurenti, 1768, and the largest and most complete vertebra is referred to the genus Vipera s.l. Laurenti, 1768 (cf. gr. “Oriental vipers”). The chelonian fossils, including some complete carapaces and plastrals, are attributed to the European pond turtle (Emys gr. Orbicularis Linnaeus, 1758) and Hermann’s tortoise (Testudo hermanni Gmelin, 1789).
Mini-kursus i den nye OJS 3.2 (Samtlige indlæg)
Authors: Frederiksen Niels Erik , Boserup Thestrup Jesper , Iversen Rie Karen Marie 
Published: June 2021

En uredigeret optagelse af webinar afholdt d. 10. juni 2021.
Her gennemgår vi:
1) Problemer med datoregistrering i QuickSubmit
2) Ændringer i den afsluttende del af det redaktionelle workflow
3) Tilretninger i artikler, der er publiceret og oprettelse af nye artikelversioner
4) Nyheder i opsætning af tidsskrift - gennemgang af 'Indstillinger'
5) Nye statistikværktøjer
Scientometric Study on Dyslipidaemia
Authors: Phull Rekha , Deshpande Vaishali , Phull Gaurav 
Published: June 2021

Dyslipidaemia an ever increasing menace emerging out of present lifestyle, is one of the major risk factors for developing Cardio-vascular abnormalities like Coronary Artery Disease (CAD), Myocardial infarction (MI), Cardiac arrest and is often associated with other metabolic disorders. Clinicians across the globe are trying hard to combat it. We have presented a Scientometric analysis of dyslipidaemia which includes Ayurvedic perspective also.
Objective: In this study we intended to study detailed Scientometric and Bibliometric study on dyslipidaemia literature.
Materials and methods: The data was collected using databases of Science- direct and Scopus, using the keywords ‘Dyslipidaemia’, ‘Dyslipidaemia in Ayurveda’, ‘Dyslipidaemia in India’,‘Medoroga in Ayurveda’ and ‘Medoraga in India’.
Results: A total of 85,500 items were retrieved from ‘science direct’ website, where majority of them were research articles and maximum publications were from the last decade indicating the recent enhancement in prevalence and awareness. A volley of research papers have come up with multiple approaches which include modern pharmacological measures, Ayurveda remedies and other herbal medicines along with non-pharmacological approaches like Yoga and Pranayama. Newer therapeutic agents like Ezetimibe, proprotein convertase subtilisin/kexin type 9 (PCSK9) monoclonal antibodies, PCSK9 small interference RNA (siRNA), and bempedoic acid, when added to the commonly used statin therapy have shown additional improvement to CV outcomes. Recent advances have come up with hepatocyte-specific targeting modifications and have shown promising lipid lowering effects.
Conclusion: Our study was probably the first such kind of work in field of dyslipidaemia which includes publications related to Ayurveda and other herbal remedies also. It shows the ever rising trends in the publications related to dyslipidaemia and its relation to other metabolic disorders in recent years. The major concern is the progression in cases of dyslipidaemia and its cardiovascular outcomes despite of having substantial research work and awareness in different streams of medicine. We must focus on the multifactorial approach encouraging behavioural, dietary and pharmacological control of cholesterol and sensitize the masses about its serious consequences. A cumulative approach including modern advances with much safer herbal remedies and practice of Yogasana and Pranayama is the need of hour and may probably bring the best solution.
Tendency of Gap Opening in Semimetal 1T′‐MoTe2 with Proximity to a 3D Topological Insulator
Authors: Zhang Cheng , Liu Wei , Zhan Fangyang , Zhang Teng , Liu Liwei , Zhang Min , Xie Sen , Li Ziwei , Sang Hao , Ge Haoran , Yan Yonggao , Wang Rui , Wang Yeliang , Zhang Qingjie , Tang Xinfeng 
Published: June 2021

AbstractMonolayer (ML) 1T′‐MoTe2 has attracted intensive interest as a fascinating quantum spin Hall (QSH) insulator. However, there are two critical aspects impeding its exploration and potential applications of QSH effects. One is its semimetallic feature with a negative band gap, leading to nontrivial edge channels annihilated by the bulk states. The other is its fabrication always accompanied by a mixed phase of 1T′ and 2H. Based on first‐principles calculations, it is shown that the large work‐function difference results in strong interlayer interactions and proximity effects in ML 1T′‐MoTe2 via interfacing a 3D topological insulator Bi2Te3, facilitating the realization of pure 1T′ phase and even the band gap opening. It is further verified that the epi‐grown ML 1T′‐MoTe2 on Bi2Te3 is nearly in single phase. Furthermore, the measurements of angle resolved photoemission spectroscopy and scanning tunneling spectroscopy confirm the obvious separated‐tendency of conduction and valence bands as well as the strong metallic edge states in ML 1T′‐MoTe2. The results also reveal the nontrivial band topology in ML 1T′‐MoTe2 is preserved in 1T′‐MoTe2/Bi2Te3 heterostructure. This work offers a promising candidate to realize QSH effects and provides guidance for controlling the nontrivial band gap opening by proximity effects in van der Waals engineering.