bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between High Immune Activity and Worse Prognosis in Uveal Melanoma and Low-Grade Glioma in TCGA Transcriptomic Data
Authors: Matsuo Hitoshi , Kamatani Takashi , Hamba Yu , Boroevich Keith A. , Tsunoda Tatsuhiko 
Published: August 2021

Abstract
Background: Immune status in the tumor microenvironment is an important determinant of cancer progression and patient prognosis. Although a higher immune activity is often associated with a better prognosis, this trend is not absolute and differs across cancer types. We aimed to give insights into why some cancers do not show better survival despite higher immunity by assessing the relationship between different biological factors, including cytotoxicity, and patient prognosis in various cancer types using RNA-seq data collected by The Cancer Genome Atlas.Results: Results showed that a higher immune activity was associated with worse prognosis in patients with uveal melanoma and low-grade glioma, which are cancers of immune-privileged sites. In these cancers, epithelial or endothelial mesenchymal transition and inflammatory state as well as immune activation had notably negative correlation with patient prognosis. Further analysis using additional single-cell data of uveal melanoma and glioma revealed that epithelial or endothelial mesenchymal transition was mainly induced in retinal pigment cells or endothelial cells that comprise the blood-retinal and blood-brain barriers, which are unique structures of the eye and central nervous system, respectively. Inflammation was mainly promoted by macrophages, and their infiltration increased significantly in response to immune activation. Furthermore, we found the expression of CCL5 was strongly correlated with immune activity and associated with bad prognosis, particularly in these cancers, suggesting that CCL5 is a potential molecular target for therapeutics.Conclusions: Epithelial or endothelial mesenchymal transition is associated with a bad prognosis. This suggests that inflammation loosens the structures of the blood barrier and causes further infiltration of immune cells, which may result in a feedback loop of additional inflammatory effects leading to runaway conditions.
Performance Analysis and Simulation of Millimeter Wave Cell-Free mMIMO Networks
Authors: Shalaby Mohamed Saeid , Hussein Hussein Mohamed , Shokair Mona Mohamed Sabry , Benaya Ahmed Mohamed 
Published: August 2021

Abstract
5G networks and beyond can provide high data rate for the served users. Small cells, massive multiple input multiple outputs (mMIMO) as well as working in millimeter wave bands are emerging tools toward empowering 5G and beyond networks. The cellular mMIMO networks can provide high data rate for users, however their performance is not satisfied for the cell-edge users and shadowed users. Fortunately, the cell-Free mMIMO network can provide a satisfied performance for all users even if they are in shadowed areas or at cell edges. The distributed access points (APs) through the coverage area can allow users to get benefit of the best serving AP. Furthermore, the users can have services anywhere due to the existence of one AP at least. The cell-Free mMIMO networks can provide a high throughput when they are operated in the millimeter wave bands due to the high available bandwidth. The operation in millimeter wave bands can let the 5G networks and beyond have a high data rate. Therefore, this paper gives a great attention to the millimeter wave bands. In this paper, the performance of the cell-Free mMIMO network, operating in the millimeter wave bands, is mathematically evaluated and simulated. The performance can include the spectral efficiency (SE), bit error rate (BER), and energy efficiency (EE). It is observed that the centralized cooperation among the APs, level 4, can provide a high SE and EE even if the maximal ratio combining (MRC) is applied. Moreover, the cell-Free four cooperation levels can perform better than cellular mMIMO when the millimeter wave non-line-of-sight (NLOS) models are applied.
An Assessment on Performance Characteristics of Diesel Engine Using Lemongrass-Diesel Oil Fuel Blends
Authors: Rana Naveen , Nagwan Harikrishna , Manickam Kannan 
Published: August 2021

Abstract
Indeed, the development of alternative fuels for use in internal combustion engines has become an essential requirement to meet the energy demand and to deal with the different problems related to fuel. The research in this domain leads to the identification of adverse fuel properties and for their solution standard limits are being defined. This paper outlines an investigation of performance and combustion characteristics of a 4-stroke diesel engine using different cymbopogon (lemongrass) - diesel fuel blends. 10% to 40% cymbopogon is mixed with diesel fuel and tested for performance characteristics like brake specific fuel consumption and brake thermal efficiency. To obtain emission characteristics smoke density in the terms of HSU has been measured. In result, it has observed that there is an increase of 5% in brake thermal efficiency and 16.33% decrease in brake specific fuel consumption. Regarding emission characteristics, a 12.9% decrease in smoke emission has been found.
Stormwater Monitoring Using On-line UV-Vis Spectroscopy for Surrogate Measurements of Chemical Contaminants and Compared With Microbiological Data
Authors: Huang Jianyin , Chow Christopher W.K. , Shi Zhining , Fabris Rolando , Mussared Amanda , Hallas Gary , Monis Paul , Jin Bo , Saint Christopher P. 
Published: August 2021

Abstract
Stormwater runoff contains a myriad of pollutants, including faecal microbes, and can pose a threat to urban water supplies, impacting both economic development and public health. Therefore, it is a necessity to implement a real-time hazard detection system that can collect a substantial amount of data, assisting water authorities to develop preventive strategies to ensure the control of hazards entering drinking water sources. An on-line UV-Vis spectrophotometer was applied in the field to collect real-time continuous data for various water quality parameters (nitrate, DOC, turbidity and total suspended solids) during three storm events in Mannum, Adelaide, Australia. This study demonstrated that the trends for on-line and comparative laboratory analysed samples were complimentary through the events. Nitrate and DOC showed a negative correlation with water level while turbidity and total suspended solids indicated a positive correlation with water level during the high rainfall intensity. The correlations among nitrate, DOC, turbidity, total suspended solids, and water level are the opposite during the low rainfall intensity. Nitrate, one of the main pollutants in stormwater, was investigated and used as a surrogate parameter for microbial detection. However, the microbiological data (E.coli) from captured storm events showed poor correlations to nitrate and other typical on-line parameters in this study, possibly explained by the nature of the stormwater catchment outside of rain events, where the sources of bacteria and nutrients may be physically separate until mixed during surface runoff as a result of rainfall. In addition, the poor correlations among the microbiological data and on-line parameters can be due to the different sources of bacteria and nutrients that end up into the stormwater drain.
The Intrapersonal and Interpersonal Processes of Fear of Recurrence Among Cervical Cancer Survivors - A Qualitative Research.
Authors: Hamama-Raz Yaira , Shinan-Altman Shiri , Levkovich Inbar 
Published: August 2021

Abstract
Objective: Cervical cancer often takes an emotional and mental toll on the affected woman. Fear of cancer recurrence was identified as one of the most prominent unmet needs in patients and survivors. The present study sought to explore meaning of fear of cancer recurrence among cervical cancer survivors owing to the paucity of studies regarding this population. Methods: In this qualitative research, semi structured interviews conducted with 15 cervical cancer survivors. Interviewees' mean age was 41.33 years (range 34-47 years) and time since diagnosis ranged from six months to seven years (mean - 3.1 years). Results: Three central themes emerged that represent intrapersonal and interpersonal processes that begin with relief from fears of cancer recurrence, alongside acknowledgement of being no longer resilient, representing the intrapersonal level. Next, an interpersonal level that included mutual fears shared by the interviewee and her partner, reflected either by coping together or coping alone. Finally, fear of recurrence became a combination of intrapersonal and interpersonal processes manifested by the greatest fear - death, expressed by both the interviewee and her family members. Conclusions: The present findings revealed that the meaning of fear of cancer recurrence represents intrapersonal and interpersonal processes encompassing three factors – uncertainty, social-cognitive processing and death anxiety. Accordingly, potential psycho-social treatment options could be tailored specific to the dominance of these factors for cervical cancer survivor.
Vagus-Preserving Proximal Gastrectomy with Double- Flap Technique for Gastrointestinal Stromal Tumor involving the Esophagogastric Junction：A Case Report
Authors: Peng Wei , Huang Yi-Kai , Wu Yong-You , Gong Wei , Chen Zheng-Rong , Chen Qiang , Su Wen-Zhao , Cheng Ming 
Published: August 2021

Abstract
Background: Gastrointestinal stromal tumor (GIST) involving the esophagogastric junction (EGJ) is a relatively rare but thorny problem. It represents a dilemma between complete resection and impairment of quality of life (QOL) for loss of cardiac sphincter or even the whole stomach. Thus, emergence of function preserving gastrectomy is necessary and may provide partial solution to this hot potato. Here, we would like to introduce a method which successfully settle this matter through a clinical case. Case presentation: A 46-year-old male complaining of a progressive dysphagia. A huge GIST with 120mm in diameter was diagnosed by enhanced CT scan and pathological exam. Imatinib mesylate (IM) was initially prescribed, the tumor was shrank to a large extent while drug resistance emerged 6 months after therapy, leaving a mass approximately 90mm in diameter. We applied a method of vagus preserving proximal gastrectomy (VP-PG) and esophagogastrostomy with double-flap technique (DFT) for the patient. The operation was smooth and postoperative courses within a 12-months follow-up were uneventful. The patient had no digestive symptoms but slight leucopenia due to IM toxicity during this medication period. Conclusion: We consider that the application of VP-PG and DFT subsequent to neoadjuvant therapy with IM is likely to be an effective and feasible option for GISTs with EGJ involvement.
Negative-Pressure Wound Therapy is Effective for Peritoneal Dialysis Catheter Exit-Site Management in The Early Postoperative Period
Authors: Fukuzaki Haruna , Nakata Junichiro , Nojiri Shuko , Shimizu Yuki , Kano Toshiki , Shirotani Yuka , Maeda Takuya , Nohara Nao , Io Hiroaki , Suzuki Yusuke 
Published: August 2021

Abstract
Peritoneal dialysis (PD) catheter exit-site care is critically important for the prevention of catheter-related infections (CRIs) and subsequent peritonitis. The postoperative management of the site is particularly essential due to its susceptibility to slow healing and infection. Despite the recent use of negative-pressure wound therapy (NPWT) for a wide variety of wounds, few studies have investigated the effectiveness of NPWT for PD catheter exit sites. In this study, 30 patients with end-stage renal disease who underwent simultaneous PD catheter insertion and exit-site formation were randomly assigned to receive NPWT (NPWT group) or conventional dressing (non-NPWT group) for the first seven postoperative days. The exit-site scores on the seventh postoperative day and over 180 days were lower in the NPWT group than in the non-NPWT group (p = 0.0049 and p = 0.007, respectively). There were no statistically significant differences between the time to first CRI and PD-related peritonitis between the two groups. There was one case of CRI with relapsing peritonitis and catheter loss in the non-NPWT group. These findings demonstrate the association between NPWT and low exit-site score. NPWT can be recommended for the management of PD catheter exit sites in the early postoperative period.
RNA Sequencing Reveals Novel LncRNAs/mRNAs Network Associated with Puerarin-mediated Inhibition of Cardiac Hypertrophy in Mice
Authors: Ye Shan , Chen Wei-Yang , Ou Caiwen , Chen Min-Sheng 
Published: August 2021

Abstract
Background: Evidence has demonstrated that puerarin is a potential drug for the treatment of cardiac hypertrophy. However, the precise underlying molecular mechanisms of the protective effect of puerarin are still unclear. Here, we aimed to explore the regulatory mechanisms of lncRNAs/mRNAs in a cardiac hypertrophy mouse model after puerarin treatment.Methods: A mouse model of cardiac hypertrophy was established by transverse aortic constriction (TAC). The echocardiography, tissue staining and western blot were used to examine the protective effect of puerarin. Then RNA sequencing (RNA-seq) was carried out to systematically analyze global gene expression. The target lncRNAs were confirmed using qRT-PCR. Moreover, a coding/non-coding gene co-expression (CNC) network was established to find the interaction of lncRNAs and mRNAs. The molecular functions, biological processes, molecular components and pathways of different expression mRNAs targeted by lncRNA were explored using Gene Ontology (GO) analysis and Kyto Encyclopedia of Genes and Genomes (KEGG) pathways analysis.Results: Puerarin exhibited obvious inhibitory effect in cardiac hypertrophy in TAC model. RNA-seq analysis was performed to investigate the lncRNAs and mRNAs expression patterns of cardiomyocytes in sham and TAC groups treated with or without puerarin. RNA-seq identified that TAC upregulated 19 lncRNAs and downregulated 18 lncRNAs, which could be revised by puerarin treatment (Fold change ≥ 3 and P< 0.05). Expression alterations of selected lncRNAs ENSMUST00000125726, ENSMUST00000143044 and ENSMUST00000212795 were confirmed by qRT-PCR. Pearson’s correlation coefficients of co-expression levels suggested that there was interactive relationship between those 3 validated altered lncRNAs and 5,500 mRNAs (r > 0.95 or r < −0.95). Those co-expressed mRNAs were enriched in some important biological processes such as vesicle-mediated transport, sin 3 complex, and translation initiation factor activity. KEGG analyses suggested that those lncRNA-interacted mRNAs were enriched in RNA transport, ribosome biogenesis in eukaryotes and proteasome signaling pathway. Conclusion: Puerarin may exert beneficial effects on cardiac hypertrophy through regulating the ENSMUST00000125726 /ENSMUST00000143044 / ENSMUST00000212795 -mRNAs network.
Plant-Soil Interactions of an Invasive Plant Species in its Native Range Help to Explain its Invasion Success Elsewhere
Authors: Aldorfová Anna , Hanzelková Věra , Drtinová Lucie , Pánková Hana , Cajthaml Tomáš , Münzbergová Zuzana 
Published: August 2021

Abstract
Purpose: To compare plant-soil feedback (PSF) of invasive Cirsium vulgare and non-invasive C. oleraceum in their native range to test a hypothesis that the invasive species is more limited by specialized pathogens in the native range and/or able to benefit more from generalist mutualists, and thus may benefit more from loss of specialized soil biota in a secondary range.Methods: We assessed changes in soil nutrients and biota following soil conditioning by each species and compared performance of plants grown in self-conditioned and control soil, from which all, some or no biota was excluded. Results: The invasive species depleted more nutrients than the non-invasive species and coped better with altered nutrient levels. The invasive species had higher seedling emergence which benefited from presence of non-specific microbes. The invasive species biomass responded less positively to specialized (self-conditioned) microbiota and more negatively to specialized larger-sized biota compared to the non-specialized control biota, suggesting the species may benefit more from enemy release and suffer less from loss of specialized mutualists when introduced to a secondary range. The invasive species showed greater ability to decrease its root-shoot ratio in presence of harmful biota and thus reduce their negative effects on its performance.Conclusions: Our study highlights the utility of detailed PSF research in the native range of species for understanding the factors that regulate performance of invasive and non-invasive species in their native range, and for pinpointing the types of biota involved in their regulation and how this changes across the plants life cycle.
Prediction of Hospital Discharge Outcome From Changes in Albumin Quotient and Cerebrospinal Fluid Lactate Dehydrogenase in Patients With Cryptococcal Meningitis
Authors: Huang Yue , Zou Jun , Zhang Ke-ming , Li Hang , Hu Dong-ying , Liao Wan-qing , Zhang Lei , Pan Wei-hua 
Published: August 2021

Abstract
BackgroundCryptococcal meningitis (CM) is a life-threatening infection of the central nervous system that occurs in healthy individuals and immunocompromised patients, and reliable prognostic parameters are needed urgently. This study analyzed the clinical characteristics and laboratory findings of CM patients from two tertiary care hospitals in China between 2015 and 2019, aiming to provide reliable prognostic factors for patients with CM.MethodsClinical characteristics and laboratory findings of CM patients were retrospectively reviewed. The unfavorable outcome was defined as no improvement or worsening symptoms and signs or persistent positive results for CSF culture or death after starting treatment. The significant differences in clinical characteristics and symptoms between the patients with favorable and unfavorable outcomes were descriptively analyzed. For comparison of mean values of laboratory results between the patients with favorable and unfavorable outcomes, the t test was performed.Results63 patients with CM were enrolled in this study and 67% were men. 38/63 patients were confirmed to be HIV serology positive. The mean onset age of all patients was 49±15 (20– 77) years. For clinical characteristics, headache, nausea and/or vomiting, and fever were the most common symptoms. Older age and disturbance of consciousness tended to poor prognosis. Changes in serum antioxidant parameters were significantly associated with clinical outcome. For CSF parameters, there were significant correlations between changes in leukocyte count, lactate dehydrogenase and chloride, and outcomes in different groups. For HIV-infected patients, increased CD4+ T cell counts suggested good prognosis. Increased CSF/serum albumin quotient (Qalb) was indicative of unfavorable outcome in HIV-negative patients.ConclusionsIn summary, CSF lactate dehydrogenase and albumin quotient may improve prediction of outcome in patients with CM. We recommend incorporation of Qalb value analysis and pay attention to change of CSF LDH during hospitalization.
Evaluation of Traits Associated With Seed Characteristics in Arkansas Restorer Lines
Authors: Azapoglu Ozgur , Srivast Vibha , Sha Xueyan , Shakiba Ehsan 
Published: August 2021

Abstract
Rice Grain dimension and weight are two critical factors for marketing and increasing yield capacity. Seed shape is measured by its length, width, thickness, and ratio of length-width. In this study, an experiment was conducted in a controlled condition from fall 2017 to 2020 to identify QTL and candidate genes associated with seed dimension and weight using a bi-parental population resulting from two University of Arkansas developed genotypes: a restorer line 367R and an advanced breeding line RU1501139, in Stuttgart, Arkansas. Five seed dimension traits, including seed length, seed width, seed thickness, seed length-width ratio, and 100-seeds weight, were obtained for QTL detection. The study detected a total of 17 QTL. Four QTL associated with seed length were identified, in which two were positioned on chr. 3, one on chr. 7, and one on chr. 11. Two QTL related to seed length-width ratio were detected on chr. 3 and 7. Whereas a total of three QTL were identified for seed thickness, one each on chr. 5, 6, and 8. Eight QTL associated with seed weight were found, of which four QTL were detected on chr. 12, two each on chr. 1 and 10, and one on chr. 3. Of 17 QTL, four QTL originated from RU1501139, while the origin of the other 13 QTL was 367R. Since multiple genes could control the yield and seed physical characteristics, the detected QTL can play a role in introducing superior parental lines for developing conventional and hybrid rice production.
The Prognostic Value of an autophagy-related lncRNA Signature in Esophageal Cancer
Authors: Zhang Zhiyong , Bi Weiyun , Zhao Yan , Ren Mudan , Li Yarui , Li Hongtao , Zhu Cailin 
Published: August 2021

Abstract
Esophageal cancer is a clinically challenging disease, seriously affecting the quality of life and prognosis of patients. Autophagy is a basic intracellular event that can maintain cell homeostasis by degrading damaged or degenerate proteins and organelles. Long noncoding RNAs (lncRNAs) are a class of RNA transcripts with a length greater than 200 nucleotides that participate in fundamental biological processes and tumor progression. However, whether autophagy-related lncRNAs can be used to predict the survival of patients with esophageal cancer still needs in-depth research. The Human Autophagy Database19 (HADb) and The Cancer Genome Atlas (TCGA) database were used to identify autophagy-related lncRNAs. Univariate and multivariate COX analyses were performed to establish a prognostic risk score model. Kaplan–Meier curves and ROC curves were carried out to evaluate the prognostic value of the autophagy-related lncRNAs signature. 1757 autophagy-related lncRNAs in esophageal cancer were identified based on specific screening criteria and four key lncRNAs (ZFAS1, AC092687.3, AC083799.1, AL080317.1) were selected to construct a novel prognostic model. Particularly, the risk score for each patient could be calculated based on the expression levels of these four lncRNAs, and a high risk score was positively associated with shorter survival time and poor prognosis of esophageal cancer patients. The autophagy-related lncRNAs signature constructed in this study can be used as a novel risk score model to predict the survival and prognosis of patients with esophageal cancer.