bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of Mitochondrial Progesterone Receptor (PR-M) in Ovarian Cancer and Its Effect on Cell Metastasis Induced by Progesterone
Authors: Yang Qingqing , Duan Chang , Wang Haofan , Jiang Dongyuan , Wang Yaping , Tian Qingfeng , Feng Quanling 
Published: March 2021

Abstract
Background: PR-M refers to a novel truncated progesterone receptor located on the outer membrane of mitochondria, capable of facilitating the proliferation of leiomyoma cells and breast cancer cells, as well as inhibiting apoptosis as impacted by progesterone or progesterone agonists. However, its role in ovarian tumors has not yet been elucidated.Objective: To study the expression of PR-M in different ovarian tumor tissues and normal tissues, and the effect exerted by progesterone on the proliferation and migration of SKOV-3 cells that achieve high PR-M expression.Methods: Real- time PCR and Western blot were employed for determining PR-M levels in cell lines, non-cancer tissues and ovarian cancer tissues. By immunohistochemistry, PR-M protein expression in benign tumor, borderline tumor and epithelial carcinoma was detected, and the clinicopathological characteristics between PR-M and cancer were analyzed. Furthermore, CCK-8 and scratch test were performed to explore the proliferation and migration of SKOV-3 cells exhibiting high PR-M expression.Results: PR-M increased significantly in cancer tissues and ovarian cancer cell lines, in comparison to normal cells and non-cancer tissues. The abnormal expression showed a significant correlation with intraperitoneal metastasis, lymph node metastasis, clinically related stage and CA125 level, suggesting that high PR-M expression may affect the progression of ovarian tumors. During the cell experiment, PR-M achieved the maximum expression in SKOV-3 cells (PR-A / B(-)). As impacted by progesterone, SKOV-3 cells (PR-A / B(-)) achieved the enhanced proliferation and migration. Besides, the enhancing effect was dose and time-dependent.Conclusion: PR-M is critical to develop ovarian cancer. Progesterone may facilitate the proliferation and migration of ovarian cancer cells exhibiting high PR-M expression.
Potential of household photobioreactor for algae cultivation
Authors: Osama Ashrakat , Hosney Hadeel , Moussa M. S. 
Published: March 2021

Abstract
Biotechnology harbors stunning potential to provide cutting-edge solutions that can mitigate arising environmental issues which impact developing countries more severely. Although considerable projects have been implemented in these developing nations to reduce the consequences of climate change, global warming, and food insecurity, most of the initiatives are deemed unsustainable or unfulfilling. Consequently, millions of individuals are still suffering from unhealthy environments and others have limited access to clean technologies. Accordingly, this manuscript is developed to act as a one-stop source for technical perspectives of microalgae cultivation and proposes the potential of scaling down of a photobioreactor (PBR) from industrial to household level to alleviate adverse environmental implications. The household PBR proposal is concerned with microalgae cultivation that contributes to mitigating CO2 from the surrounding environment and synthesizing a product that could be of high nutritional value. Additionally, the business model of household PBR is developed in accordance to low and middle-income countries’ demands to facilitate future projects in this scope. The value proposition of this model relies on decreasing climate change impacts, enhancing wellbeing, and providing natural supplements. Scale of economy, appropriate technology, and socio-economic challenges for household PBR are highlighted.
Donor-transmitted Anterior Myocardial Ischaemia in a Teenager: How to Proceed?
Authors: Göbölös Laszlo , Hogan Maurice , El-Banna Mosaad , Bader Feras , Tuzcu Emin Murat , Bhatnagar Gopal 
Published: March 2021

Background:
Heart transplantation remains the treatment of choice for end-stage heart failure patients, owing to the associated dual improvements in quality of
life, and prognosis. The discrepancy between higher demand and supply of donor organs is the limiting factor, and is established universally.
Increasing consideration of donor populations up to 65 years of age and marginal donor hearts has helped to facilitate the availability of potential
grafts. However, grafts from older donors carry the mid-term increased risk of coronary allograft vasculopathy, including donor-transmitted
coronary disease.

Case report:
A 15-year-old female underwent orthotopic heart transplantation for non-ischaemic cardiomyopathy, the donor was a 44-year-old male. The
recipient developed anterior wall ischaemia within a year requiring coronary angioplasty and stent implantation to treat the severe obstruction in
the left anterior descending coronary artery. However, two months later, the patient was readmitted with in-stent restenosis.
Therefore, to optimally revascularise the left anterior descending coronary artery, and minimise risks associated with re-sternotomy, a minimally
invasive direct coronary artery by pass grafting of the left internal mammary artery to the left anterior descending artery was performed.

Conclusion:
Surgical revascularisation in generalised coronary allograft vasculopathy is an inadequate option; repeat heart transplantation is the treatment of
choice, albeit given its morbidity, should be reserved for a highly selected patient population. In localised coronary lesions, conventional coronary
bypass surgery may be a feasible choice in selected patients with left anterior descending artery lesions. Minimal invasive techniques, such as
minimally invasive direct coronary artery bypass grafting rather than robotic techniques, would be preferable for ease of approach and to limit the
surgical re-do trauma.
A New Prognostic Strategy Based on four DNA Repair-Associated
lncRNAs for Hepatocellular Carcinoma
Authors: Liu Li , Zeng Hanyi , Liu Chengdong , Zhou Xiaohan 
Published: March 2021

Background:
Hepatocellular carcinoma (HCC) is a malignant tumour with a poor
prognosis. The effect of DNA repair on prognosis cannot be ignored, and long non-coding RNA
(lncRNA) can regulate the DNA repair process.

Objective: :
To obtain DNA repair-associated lncRNA (DR-lncRNA) prognostic signature for
improving the ability to predict HCC prognosis.

Methods:
Our study used the Cancer Genome Atlas database. Gene set variation analysis was
performed to differentiate high and low levels of DNA repair to identify DR-lncRNAs. By
performing univariate Cox regression, LASSO regression, and multivariate Cox regression
analyses, we finally obtained a DR-lncRNA prognostic signature and constructed a nomogram
prognostic model. Time-dependent receiver operating characteristic (ROC) curves, calibration
plots, decision curve analysis (DCA), and clinical impact curves were used to assess predictive
ability and clinical utility. Differentially expressed genes (DEGs) functional enrichment analysis
was performed to further explore the underlying mechanisms that influence HCC prognosis.

Results:
We obtained the following DR-lncRNA prognostic signature:AP002478.1, AC116351.1,
LINC02580, and LINC00861. The ROC curves and calibration plots showed good discrimination
and calibration properties. Combining the DR-lncRNA prognostic signature and tumour stages, we
established a nomogram prognostic model. DCA and clinical impact curves showed the clinical
utility of this model. DEGs of high-risk and low-risk groups predicted by the prognostic DRlncRNA
were significantly associated with cell cycle, various metabolic pathways and biological
processes, such as the oxidation-reduction process and cell division.

Conclusion:
We identified a DR-lncRNA prognostic signature and constructed a nomogram
prognostic model, which could be a beneficial prognostic strategy for HCC.
Relationship Between Clinical, Demographic and Socioeconomic Factors
with Suicide Ideation; A Cross-sectional Study
Authors: Ghazizadeh Hamideh , Khorasani Mahdiyeh Yaghooti , Shabani Niloofar , Sahranavard Toktam , Zare-Feyzabadi Reza , Mohammadi-Bajgiran Maryam , Timar Ameneh , Oladi Mohammad Reza , Moazedi Sara , Mosalman-Zadeh Negin , Khedmatgozar Hamed , Rohban Mohadeseh , Hasanzadeh Elahe , Javandoost Ali , Nazarpour Shahin , Bahrami Afsane , Ferns Gordon A. , Haghdoost Ali Akbar , Esmaily Habibollah , Ghayour-Mobarhan Majid 
Published: March 2021

Background:
Suicide has grown in global prevalence as a public health problem. We
aimed to evaluate the association of socioeconomic factors, biochemical and hematologic tests, and
suicide ideation.

Methods:
In this cross-sectional study, 8267 Iranian adults aged 35 – 65 years old were enrolled.
The assessment of suicide ideation was made by the completion of Beck’s depression inventory
(BDI) questionnaire; according to one specific item on the questionnaire: “have you ever decided
to suicide in the past week?”

Results:
According to our results, 6.9 % of subjects had ideation of suicide. The results showed
high levels of FBG, RBC, MCHC, and hs-CRP were associated with suicide ideation. Obese,
single subjects, and current-smokers had a higher risk of suicide ideation.

Conclusion:
Increased physical activity, obesity, and smoking are associated with a high risk of
suicide ideation; whilst, a high MCHC is related to a low risk of suicide ideation in Iranian adults.
The Long Non-coding RNA Cytoskeleton Regulator (CYTOR) Sponges microRNA- 206 (miR-206) to Promote Proliferation and Invasion of HP75 Cells
Authors: Keqi Hu , Handong Liu 
Published: March 2021

Background:
The role and mechanism of long non-coding RNA cytoskeleton regulator
(CYTOR) in Invasive Pituitary Adenomas (IPA) have not been elucidated previously.

Objective:
This study aimed to investigate the interaction between CYTOR and miR-206 and their
roles in IPA using HP75 cells as the model.

Methods:
The expression levels of CYTOR and miR-206 were detected by quantitative real-time
polymerase chain reaction (qRT-PCR) in IPA tissues and cell lines. The Chi-square test was used
to analyze the correlation between CYTOR expression and clinical-pathological parameters. HP75
cell proliferation was detected by Cell Counting Kit-8 assay and colony formation assay. Scratch
healing experiments and Transwell assay were used to detect migration and invasion of HP75 cells.
The relationship between CYTOR and miR-206 was predicted by bioinformatics and verified by
qRT-PCR and the dual-luciferase reporter gene method.

Result:
CYTOR is up-regulated in IPA tissues and cell lines. The high expression of CYTOR is
associated with adenoma invasiveness and adenoma size of the patients. Down-regulation of CYTOR
decreases the proliferation, migration and invasion of HP75 cells, while up-regulation of
miR-206 can inhibit proliferation, migration and invasion of HP75 cells. MiR-206 is identified as a
target of CYTOR and could be negatively regulated by it in IPA.

Discussion:
CYTOR, as a tumor-promoting factor, facilitates the proliferation, migration and invasion
of HP75 cells through sponging miR-206.

Conclusion:
The CYTOR-miR-206 axis provides new insights into the diagnosis and treatment of
IPA.
By Discontinuing Beta-Blockers Before an Exercise Test, We may Precipitate a Rebound Phenomenon
Authors: Koracevic Goran , Micic Sladjana , Stojanovic Milovan 
Published: March 2021

Background:There is a need to analyse the current approach to beta-blocker (BB) use in relation to exercise-based stress tests.Objective:We compared various guidelines regarding recommending abrupt vs. gradual discontinuation of BB prior to exercise tests. We also analyse the shortcomings of the currently recommended approach and suggest a new approach to avoid BB rebound.Methods:A narrative review is used to analyse this topic due to lack of valid randomized clinical trials.Results:Omitting the BB therapy prior to exercise-based test has been recommended in guidelines for many years. Although reasonable, this approach has potential disadvantages since sudden BB withdrawal may induce a rebound phenomenon, which is also acknowledged in several guidelines.Conclusions:We observed inconsistency among relevant guidelines; there is no homogenous approach regarding BB use before exercise tests. Most guidelines recommend BB withdrawal for a couple of days before the test; they do not advise BB dose tapering. This approach is not standardised and raises the risk of BB rebound phenomenon before and during the test. Therefore, we suggest using half the prescribed BB dose at the usual time of administration (in the morning, prior to the exercise test).
Diselenides and Selenocyanates as Versatile Precursors for the Synthesis of
Pharmaceutically Relevant Compounds
Authors: Kostić Marina D. , Divac Vera M. 
Published: March 2021

:
Organoselenium chemistry has undergone extensive development during the past decades, mostly due to the unique chemical properties of organoselenium compounds that have been widely explored in a number of synthetic transformations, as well as due to the interesting biological properties of these compounds. Diselenides and selenocyanates constitute the promising classes of organoselenium compounds that possess interesting biological effects and that can be used in the preparation of other selenium compounds. The combination of diselenide and selenocyanate moieties with other biologically relevant molecules (such as heterocycles, steroids, etc.) is a way for the development of compounds with promising pharmaceutical potential. Therefore, the aim of this review is to highlight the recent achievements in the use of diselenides or selenocyanates as precursors for the synthesis of pharmaceutically relevant compounds, preferentially compounds with antitumor and antimicrobial activities.
The Recovery of an Elderly Woman with Asthma and Diabetes from
COVID-19 Infection: A Case Report
Authors: Ghaysouri Abas , Safari Maryam , Mohammadyari Elham , Balavandi Firooz , Nazari Ali , Feizi Jalil , Karimian Mohamad , Bastani Elham , Salimi Ebrahim , Tavan Hamed 
Published: March 2021

Introduction:
COVID-19 has caused a great deal of fear indifferent communities. In this study, we reported the recovery of a diabetic elderly woman with a history of asthmafromCOVID-19 infection.

Case Presentation:
A 61-year-old woman was hospitalized due to dyspnea and low blood O2 saturation and later diagnosed with COVID-19, as confirmed by PCR. The patient had a history of asthma and diabetes and used salbutamol and Atrovent spray. She also had a history of hospitalization at one month prior to the current admission. Despite all these, COVID-19was successfully managed, and she was discharged with a good clinical condition after the improvement of respiratory complications.

Conclusion:
Despite having an advanced age and underlying diseases (diabetes and asthma), the recovery of this elderly woman shows that even such patientscandefeatCOVID-19 as long as the disease is not progressed to advanced phases and appropriate therapeutic measures are taken. By reporting such cases, it is possible to give people hope and motivation and reduce their fear of the disease.
Radiomics - Quantitative Biomarker Analysis for Breast Cancer Diagnosis and Prediction: A Review
Authors: Moyya Priscilla Dinkar , Asaithambi Mythili 
Published: March 2021

Background:
Breast cancer has become a global problem. Though concerns regarding
early detection and accurate diagnosis have been raised, continued efforts are required for the development
of precision medicine. In the past years, the area of medicinal imaging has seen an unprecedented
growth that has led to an advancement of radiomics, which provides countless quantitative
biomarkers extracted from modern diagnostic images, including a detailed tumor characterization
of breast malignancy.

Discussion:
In this review, we have presented the methodology and implementation of radiomics
together with its future trends and challenges on the basis of published papers. Radiomics could
distinguish malignant from benign tumors, predict prognostic factors, molecular subtypes of breast
carcinoma, treatment response to neoadjuvant chemotherapy (NAC), and recurrence survival. The
incorporation of quantitative knowledge with clinical, histopathological, and genomic information
will enable physicians to afford customized care of treatment for patients with breast cancer.

Conclusion:
This review was intended to help physicians and radiologists gain fundamental knowledge
regarding radiomics, and also to work collaboratively with researchers to explore evidence for
its further usage in clinical practice.
Metabolic Characteristics of SM-1, a Novel PAC-1 Derivative, in Human Liver Microsomes
Authors: Gong Ya , Wang Peiqi , Li Jianming , Jingbinin Huang , Ding Jinsong 
Published: March 2021

Background and Objectives:
SM-1 is a new synthetic small molecule compound with antitumor activity. The metabolism of SM-1 is a key parameter that needs to be evaluated to provide further insight into drug safety and efficacy in the early phases of drug development.

Methods and Results:
In this study, the biotransformation process of SM-1, including the metabolic pathways
and major metabolites, was investigated based on a liquid chromatography-mass spectrometry
method. Upon incubation of SM-1 with human liver microsomes, five metabolites were identified,
namely dihydrodiol formation (R1), hydroxylation (R2, R3, and R5), and debenzylation (R4)
of SM-1, with R1 and R4 being the major metabolites. The enzyme kinetic parameters of SM-1
were determined by a liquid chromatography-tandem mass spectrometry method. The enzyme kinetics
of SM-1 obeyed the Michaelis-Menten equation. The Vmax, Km, and CLint of SM-1 in HLMs
were 14.5 nmol/mg protein/h, 6.32 μM, and 2.29 mL/mg protein/h, respectively.

Results:
The chemical inhibition studies showed that CYP450 isoenzymes were responsible for
SM-1 metabolism in HLMs, and CYP3A4 was the major CYP450 isoenzyme involved in the metabolism
of SM-1; these findings were confirmed by using the human recombinant CYP3A4.

Conclusion:
Through the identification of the biotransformation pathways and enzyme kinetics of
SM-1, the metabolic enzymes for SM-1 in HLMs are characterized.
Hepatitis B Virus X Protein Modulates Chemokine CCL15 Upregulation in Hepatocellular Carcinoma
Authors: Li Ying , Wang Chaomin , Zhao Ting , Cui Ranliang , Hu Linfei , Chang Luchen , Wei Xi , Zhang Jin , Li Yueguo 
Published: March 2021

Background:
Hepatitis B virus X protein (HBx) is an indispensable progression factor in Hepatocellular
Carcinoma (HCC). CCL15 could be a peculiar proteomic biomarker of HCC with tumorigenesis and tumor
invasion.

Objective:
The aim of study was to explore the relationship between HBx and CCL15 expression in HCC.

Methods:
HBV-positive HCC pathological tissue samples and corresponding adjacent non-tumor liver tissues
were collected. The expression of HBx and CCL15 was analyzed by immunohistochemistry, real-time Polymerase
Chain Reaction (PCR), and western blot analysis in tissues or in vitro.

Results:
The levels of CCL15 mRNA and protein expression in HCC samples were observably higher than
those of adjacent non-tumor liver tissues. The CCL15 was significantly associated with the expression of HBx
in HBV-positive HCC samples. The up-regulation of HBx induced CCL15 expression in vitro. The high expression
score of CCL15 was significant associated with the poor prognosis of HCC patients.

Conclusions:
The CCL15 expression was observably associated with HBx in HCC patients. The CCL15 may be
considered as an indicator in the clinical management of HBV-associated HCC.
PERAN GURU SEJARAH DALAM PENDIDIKAN KARAKTER DI ERA REVOLUSI INDUSTRI 4.0
Authors: Suyanti Suyanti 
Published: March 2021

Guru adalah pendidik profesional dengan tugas mengajar, mendidik, membimbing, melatih, dan menilai peserta didik. Peran guru sejarah semakin kompleks pada era Revolusi 4.0. Guru bukan hanya dituntut untuk menguasai materi pembelajaran maupun kompetensi penunjang pembelajaran, namun guru juga dituntut untuk mananamkan karakter nasionalisme. Di era revolusi 4.0 perkembangan teknologi semakin cepat dan guru sejarah diharapkan tidak hanya memberikan ilmu pengetahuan saja tetapi harus menginternalisasi nilai-nilai karakter yang tidak bisa digantikan oleh teknologi secanggih apapun. Maka dari itu, sebagai guru sejarah harus bisa mengembangkan kompetensinya mengikuti perkembangan zaman dan bisa memainkan peran demi keberhasilan belajar peserta didik.Kata kunci: Guru sejarah, pendidikan karakter, Revolusi Industri 4.0
Branding Cannabis in Canada: Challenges for theCannabis Act's Promotion Restrictions
Authors: Asquith Kyle 
Published: March 2021

Background:This article examines how Canadian cannabis companies promoted their new brands after legalization in late-2018. Analysis:Nearly 4,000 items were collected from the websites and social media of 20 cannabis brands and triangulated with insight from the trade press. The promotional practices are contextualized in two areas: the history of tobacco advertising in Canada, as legal precedent for the Cannabis Act, and theories of branding. Conclusion and implications:Brands are navigating the Cannabis Act’s promotion restrictions by embodying what it means to be a brand in the twenty-first-century media environment. This reveals an incompatibility between regulations and contemporary marketing.
DISAIN PEMBELAJARAN ONLINE PADA ERA DAN PASCA COVID-19
Authors: Marbun Purim 
Published: March 2021

<em>Penelitian ini dilatarbelakangi situasi dan kondisi pada masa covid-19 mengharuskan setiap orang melakukan kegiatan di rumah. Pemerintah telah menetapkan policy bahwa semua masyarakat stay at home, termasuk kegiatan belajar, bekerja dan beribadah pun dilakukan dari rumah. Dengan instruksi di atas implikasi bagi kegiatan pembelajaran memerlukan disain pemnbelajan online untuk memastikan berlangsungnya pendidikan dengan baik. Metode penelitian dalam tulisan ini ialah studi kepustakaan dengan fokus menemukan model-model disain pembelajaran yang efektif dan dapat di gunakan pada era dan pasca covid-19. Hasil penelitian ini diharapkan mampu memberikan pilihan-pilihan model disain pembelajaran online kepada dosen sehingga mampu menyajikan pembelajaran secara efektif dan berkualitas.</em>