bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal clonal dynamics of HIV-1 latent reservoirs measured by combination quadruplex polymerase chain reaction and sequencing
Authors: Cho Alice , Gaebler Christian , Olveira Thiago , Ramos Victor , Saad Marwa , Lorenzi Julio , Gazumyan Ana , Moir Susan , Caskey Marina , Chun Tae-Wook , Nussenzweig Michel 
Published: October 2021

ABSTRACTHIV-1 infection produces a long-lived reservoir of latently infected CD4+ T cells that represents the major barrier to HIV-1 cure. The reservoir contains both intact and defective proviruses, but only the proviruses that are intact can re-initiate infection upon cessation of antiretroviral therapy (ART). Here we combine 4 color quantitative polymerase chain reaction and next-generation sequencing (Q4PCR) to distinguish intact and defective proviruses and measure reservoir content longitudinally in 12 infected individuals. Q4PCR differs from other PCR based methods in that the amplified proviruses are sequence verified as intact or defective. Samples were collected systematically over the course of up to 10 years beginning shortly after the initiation of ART. The size of the defective reservoir was relatively stable with minimal decay during the 10-year observation period. In contrast, the intact proviral reservoir decayed with estimated half-life of 4.9 years. Nevertheless, both intact and defective proviral reservoirs are dynamic. As a result, the fraction of intact proviruses found in expanded clones of CD4+ T cells increases overtime with a concomitant decrease in overall reservoir complexity. Thus, reservoir decay measurements by Q4PCR are quantitatively similar to viral outgrowth (VOA) and intact proviral DNA PCR (IPDA) with the addition of sequence information that distinguishes intact and defective proviruses and informs reservoir dynamics. The data is consistent with the notion that intact and defective proviruses are under distinct selective pressure, and that the intact proviral reservoir is progressively enriched in expanded clones of CD4+ T cells resulting in diminishing complexity over time.SignificanceHIV-1 infection requires lifelong treatment with antiretroviral therapy (ART) due to viral rebound of a latent reservoir of intact, transcriptionally silent provirus found to persist in the genome of CD4+ T cells. One of the major challenges to understanding the nature of the latent reservoir is accurately characterizing the measuring the size of the reservoir. Herein, we use quadruplex polymerase chain reaction (Q4PCR) to assess the dynamics of the latent reservoir in HIV+ individuals who have been on long-term ART for up to 10 years. Our results show that Q4PCR can be used to accurately measure the latent reservoir, while providing the added benefit of assessing the genetic diversity of the reservoir to better understand changes to clonal dynamics overtime.
Recording true oxygen reduction capacity during photosynthetic electron transfer in Arabidopsis thylakoids and intact leaves
Authors: Fitzpatrick Duncan , Aro Eva-Mari , Tiwari Arjun 
Published: October 2021

AbstractReactive oxygen species (ROS) are generated in electron transport processes of living organisms in oxygenic environments. Chloroplasts are plant bioenergetics hubs where imbalances between photosynthetic inputs and outputs drive ROS generation upon changing environmental conditions. Plants have harnessed various site-specific thylakoid membrane ROS products into environmental sensory signals. Our current understanding of ROS production in thylakoids suggests that oxygen (O2) reduction takes place at numerous components of the photosynthetic electron transfer chain (PETC). To refine models of site- specific O2 reduction capacity of various PETC components in isolated thylakoids, the stoichiometry of oxygen production and consumption reactions, associated with H2O2 accumulation, was quantified using membrane inlet mass spectrometry and specific inhibitors. Combined with P700 spectroscopy and electron paramagnetic resonance spin trapping, we demonstrate that electron flow to PSI is essential for H2O2 accumulation during light-induced photosynthetic electron transport process. Further leaf disc measurements provided clues that H2O2 from PETC has a potential of increasing mitochondrial respiration and CO2 release.One sentence summaryPhotosynthetically derived H2O2 only accumulates at Photosystem I and may trigger cooperation with mitochondria during stress
Mesodiencephalic junction Gabaergic inputs are processed separately from motor cortical inputs in the basilar pons
Authors: Khalil Ayoub J. , Mansvelder Huibert D. , Witter Laurens 
Published: October 2021

AbstractThe basilar pontine nuclei (bPN) receive inputs from the entire neocortex and constitute the main source of mossy fibers to the cerebellum. Despite their critical position in the cortico-cerebellar pathway, it remains unclear if and how the bPN process inputs. An important unresolved question is whether the bPN strictly receives excitatory inputs or also receives inhibitory inputs. In the present study, we identified the mesodiencephalic junction as a prominent source of GABAergic afferents to the bPN. We combined optogenetics and whole-cell patch clamp recordings and confirmed that the bPN indeed receives monosynaptic GABA inputs from this region. Furthermore, we found no evidence that these inhibitory inputs converge with motor cortex (M1) inputs at the single neuron level. We also found no evidence of any connectivity between bPN neurons, suggesting the absence of a local circuit. Finally, rabies tracings revealed that GABAergic MDJ neurons themselves receive prominent inputs from neocortical output neurons. Our data indicates that inhibition from the MDJ, and excitation from the neocortex remain separate streams of information through the bPN. It is therefore unlikely that inhibition in the bPN has a gating function, but rather shapes an appropriate output of the bPN during behavior.
From appearance comparison to fitness intention: mediating roles of appearance-based exercise motivation and perceived behavioral control
Authors: Pu Bo , Zhang Lu , Sang Wenyuan , Ji Siyu 
Published: October 2021

PurposeThis study aims to explore the influence of appearance comparison on fitness intention. Specifically, it analyzes the mediating effect of appearance-based exercise motivation and perceived behavioral control between appearance comparison and fitness intention.Design/methodology/approach434 samples were obtained by the network survey in China. Hierarchical regression analysis and the Hayes' SPSS PROCESS macro were used to verify the hypotheses.FindingsAppearance comparison has a positive influence on fitness intention. Appearance-based exercise motivation mediates appearance comparison and fitness intention. Appearance comparison can produce a positive effect on fitness intention via appearance-based exercise motivation and perceived behavioral control in sequence.Practical implicationsThe findings have some practical implications for both individuals and fitness center managers. First, people can view appearance comparison rationally, understand the process of its transformation into fitness intention and enhance fitness intention. Second, fitness center managers can make some reasonable marketing plans according to this study.Originality/valueThis study explores the positive effects of appearance comparison combining social comparison theory, social cognitive theory and the theory of planned behavior. It contributes to extant literatures about appearance comparison and fitness intention by promoting the understanding of the influence mechanism of fitness intention.
Perceived effectiveness of e-governance as an underlying mechanism between good governance and public trust: a case of Indonesia
Authors: Hartanto Dadang , Dalle Juhriyansyah , Akrim A. , Anisah Hastin Umi 
Published: October 2021

Purpose
This study aims to investigate the association of perceived accountability, perceived responsiveness and perceived transparency, and public trust in local government. Additionally, mediation of the perceived effectiveness of e-governance was also tested between these relationships.

Design/methodology/approach
Using a quantitative cross-sectional field survey, primary data was collected at local administration levels from two cities in Indonesia. The final data set of 355 respondents was then analyzed using SmartPls3 and the measurement and structural models were tested.

Findings
Positive results were obtained for all the hypothesized links (direct and indirect relationships). The study’s findings revealed useful insights for policymakers and researchers regarding the public’s perception of good governance and their expectations from the government, which further lead toward trust in local governments.

Practical implications
The study concluded that good governance practices develop and enhance the public’s trust in the government, thus provided key policy directions.

Originality/value
This study contributes to the body of knowledge related to good governance elements and their impact on public trust in the local government via the underlying mechanism of perceived e-governance effectiveness in developing countries in general and particularly the Indonesian context. Moreover, it is a unique study in the good governance domain while considering three good governance elements into a single theoretical framework. Previous studies have explored these elements individually with public trust, so this combined framework advances the body of knowledge. This research’s findings also contributed toward validating good governance theory with e-governance effectiveness and public trust integration in a single comprehensive framework. This research also helped answer the questions arising from past literature about declining public trust trends in local governments.
Entrepreneurial influencers and influential entrepreneurs: two sides of the same coin
Authors: Guiñez-Cabrera Nataly , Aqueveque Claudio 
Published: October 2021

PurposeDrawing on push and pull entrepreneurship theory, this research investigates how and why social media users become social media influencers (SMIs), a specific type of digital entrepreneur.Design/methodology/approachAdopting a phenomenological perspective and following a process approach, a total of 35 semi-structured face-to-face interviews were conducted with SMIs of different ages, follower numbers and associated with diverse areas of expertise. Subsequently, via interpretative analysis of interviewees' narratives and reasons for becoming SMIs, relevant motivations and events were uncovered and described.FindingsThe findings showcase two types of SMIs: “Entrepreneurial Influencers” and “Influential Entrepreneurs”. Their motivations and the path they followed on their entrepreneurial efforts were also uncovered. Finally, based on these findings, a new entrepreneurial motivational driver is proposed.Practical implicationsPublic entrepreneurial incentive policies should consider SMIs as a specific type of would-be entrepreneurs with some advantage in terms of prominence and reputation, which might help them to successfully initiate and consolidate traditional entrepreneurial activities.Originality/valueThis paper is among the first to examine SMIs from an entrepreneurial perspective, contributing to the nascent digital entrepreneurship literature.
Digitalization and rural entrepreneurial attitude in Indonesia: a capability approach
Authors: Fahmi Fikri Zul , Savira Medina 
Published: October 2021

Purpose
This paper aims to identify how digitalization affects entrepreneurial attitudes in rural areas in Indonesia, a country in the Global South. The development of digital technology can help entrepreneurs, in that faster and easier information acquisition helps rural communities to identify new opportunities and innovate. Yet, digital development generates higher disparity, and thus, not all people can benefit from digitalization. Although digital technology can facilitate the development of entrepreneurship, its benefits depend on individual preferences. In this regard, the capability approach is used so as to reflect how different valuations of digital technology in rural entrepreneurs influence their attitude with regard to recognizing business opportunities and taking risks.

Design/methodology/approach
A double case study approach is used in which this study examines two cases of rural entrepreneurs in Indonesia that represent different uses of digital technologies and socio-economic rural contexts: coffee entrepreneurs in Kintamani and craft producers in Kamasan village. In so doing, semi-structured interviews were conducted with local entrepreneurs and communities according to purposive and snowball sampling techniques. The qualitative data were then analysed using a constant comparative technique which allows us to develop a conceptual argument by observing patterns within and between the cases.

Findings
The findings show that digitalization shapes the attitude of rural entrepreneurs differently, although the same opportunity from using digital technology is present in the village. Social and environmental factors facilitate the rural entrepreneurs to consider using digital technology to develop their businesses. However, as each individual entrepreneur has a different valuation of digital technology, the benefits it offers – such as broader market opportunities and new business ideas – vary. Entrepreneurs who consider digital technology to be a valuable resource for developing their businesses are more curious to explore its benefits.

Originality/value
The capability approach provides a new perspective in understanding rural entrepreneurship. First, the authors demonstrate that the success of rural entrepreneurship is influenced not only by concrete things (e.g. resources) but also the individual perspective on these resources which may vary across entrepreneurs. Second, the authors show not only the potential differences in socio-cultural contexts in which the capability approach is applied but also how socio-cultural values and collectivism influence the individual valuation of resources that could benefit entrepreneurs.
Does Islamic values matter for Indonesian students’ entrepreneurial intention? The mediating role of entrepreneurial inspiration and attitude
Authors: Wibowo Agus , Widjaja Sri Umi Mintarti , Utomo Sugeng Hadi , Kusumojanto Djoko Dwi , Wardoyo Cipto , Wardana Ludi Wishnu , Narmaditya Bagus Shandy 
Published: October 2021

Purpose
Stimulating new business creating has been a decisive issue and the Indonesian Government is responding to this issue by providing entrepreneurship programs for Islamic students. This study aims to examine the impact of Islamic values and entrepreneurship education to predict Islamic students’ intention for business, as well as investigates the mediating role of entrepreneurial inspiration and attitudes.

Design/methodology/approach
The study estimates primary data obtained from a sample of 381 Islamic boarding students in a selected area in Indonesia who incorporated entrepreneurial education and practices. In addition, structural equation modeling has been incorporated to answer the hypotheses raised in the research.

Findings
The findings strengthen the study of the influence of Islamic values that affects both directly and through inspiration and motivation on students’ entrepreneurial intentions. Additionally, it found the direct and indirect influences of entrepreneurship education on intentions for entrepreneurship through inspiration and motivation.

Research limitations/implications
This study has been cramped to draw a limited geographical area in Indonesia and the research’s implication is used for relevant themes in Islamic entrepreneurship.

Practical implications
The findings of this research give insights to promote students’ entrepreneurial intention from Islamic schools.

Social implications
The findings of this study offer a broader knowledge to enhance entrepreneurs through economic education in Islamic boarding schools.

Originality/value
These results provide insights into the important effects of Islamic values, particularly in the context of Islamic boarding schools. The findings suggest that Islamic values be an important contextual factor for Islamic boarding school students’ entrepreneurial intentions.
Together or alone on the prosocial path amidst the COVID-19 pandemic: the partitioning effect in experiential consumption
Authors: Cho Yoon-Na , Kim Ha Eun , Youn Nara 
Published: October 2021

PurposeDuring these unprecedented times, acts of charity are deemed essential to help individuals in need and support the social safety net. Given the importance of prosocial behavior for survival through the coronavirus disease 2019 (COVID-19) outbreak, the authors investigate the effects of partitioning experiential consumption and self-construal on consumer responses.Design/methodology/approachBased on the literature on partitioning and self-construal, the findings across three experimental studies provide novel insights into the interplay between partitioning and self-construal, and offer psychological processes on prosocial and behavioral intention.FindingsIndividuals with predominantly independent (vs. interdependent) self-construals and those primed with independent (vs. interdependent) self-construals showed higher prosocial intention when the experiential product ad was in an aggregated (vs. partitioned) format. The fit between the type of format and self-construal leads to the high control coping mechanism, and ultimately prosocial intention.Originality/valuePartitioning experiential consumption has not been directly examined using self-construal, providing novel insights into consumer reactions during the pandemic. This paper provides practical implications to practitioners and researchers to better understand and adapt to shifting digital consumption patterns.
Webrooming or showrooming? The moderating effect of product attributes
Authors: Guo Yan , Zhang Min , Lynette Wang Valerie 
Published: October 2021

PurposeThis study examines consumers' channel attitudes and choices leading to webrooming and showrooming, and how product attributes (informational vs experiential and perceived risk) moderate the effects of channel attitudes.Design/methodology/approachA research framework is built upon the heterogeneity of channel attitudes, the lack of intrachannel lock-in and interchannel synergy. A questionnaire-based survey yields 868 multi-channel consumer responses in China. Simultaneous equation modeling and STATA 12.0 are used to test the hypotheses.FindingsConsumers webroom when buying high-risk informational products (e.g. personal computers or mobile phones). They webroom as well as showroom for high-risk experiential products (e.g. clothing or cosmetics). Moreover, a single channel is preferred to webrooming or showrooming for purchasing low-risk informational (e.g. books or stationery) and low-risk experiential (e.g. snacks or toys) products. The results also show that webrooming is more frequently used than showrooming by consumers.Research limitations/implicationsThis study extends current understanding on multi-channel and omnichannel shopping behavior and highlights the role of product attributes in customer journey mapping.Practical implicationsThis study offers retailers and other downstream firms a fresh perspective on multi-channel customer experience management and channel design.Originality/valueThis study offers a clear explanation on the commonalities and differences between webrooming and showrooming.
Factors affecting the adoption of blockchain technology in innovative Italian companies: an extended TAM approach
Authors: Sciarelli Mauro , Prisco Anna , Gheith Mohamed Hani , Muto Valerio 
Published: October 2021

PurposeThe present research aims to identify the determinants for users' behavioral adoption of Blockchain, exploring the relationships among these variables and investigating whether the proposed model can provide a more comprehensive manner to understand the adoption of Blockchain technology.Design/methodology/approachThis study adopts the Technology Acceptance Model (TAM) approach and extends it with external constructs: “reduced cost” and “efficiency and security”. This paper used a quantitative and exploratory approach through the collection and analysis of data from a total of 108 Italian innovative SME. We have used the Partial Least Squares Structural Equation modeling (PLS-SEM) approach using SmartPLS for model evaluation.FindingsThe results show that “efficiency and security” is an important driver of firms' decision-making process to adopt Blockchain. Moreover, the results show that perceived usefulness is a strong predictor of the intention to use Blockchain in business processes.Originality/valueThis research advances the literature on technology adoption in business processes, focusing on a particular technology: Blockchain. The field has been strengthened by investigating the determinants of technology adoption, adding new perspectives; both reduced cost and efficiency, and security.
User trends of electronic payment systems adoption in developing countries: an empirical analysis
Authors: Al-Sabaawi Mohmed Y. Mohmed , Alshaher Ali Abdulfattah , Alsalem M.A. 
Published: October 2021

PurposeElectronic payment (e-payment) systems literature analysis reveals that they are growing in developing countries; however, they are limited in the Arab countries and, more importantly, scarce in Iraq in particular. Therefore, this paper aims to investigate the factors influencing the intention of users to use e-payment systems in Iraq. Additionally, this study proposes an e-payment adoption model based on technology usage models to identify user trends toward e-payment systems.Design/methodology/approachA quantitative approach is adopted to test the proposed model. The proposed model is based on the Unified Theory of Acceptance and Usage of Technology theory. The proposed model is validated using survey data from 339 e-payment system users. Using Amos software, this study used structural equation modeling (SEM), a statistical technique for analyzing factor relationships.FindingsThe findings of the study indicate that performance expectancy, effort expectancy, social influence, facilitating conditions and price saving orientation influence Intention to accept the e-payment system. Similarly, habit, technology security, trust, innovation resistance, psychological empowerment also affect intention to accept an e-payment system. However, hedonic motivation and perceived risk do not affect e-payment system adoption.Originality/valueThe identified factors play a major role in user intentions toward the adoption of e-payment systems for financial transactions and addressing these factors will make e-payment acceptable in the future. The results of this study contribute to assisting governments or e-payment firms and decision-makers in building strategic decisions or policies that will increase the adoption of e-payment by individuals.