bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implication of immune cell signature of tumor microenvironment in diffuse large B‐cell lymphoma
Authors: Yu Hao , Fu Di , Xu Peng‐Peng , Cheng Shu , Wang Li , Zhang Yun‐Zeng , Zhao Wei‐Li 
Published: July 2021

AbstractDiffuse large B‐cell lymphoma (DLBCL) is a genetically heterogeneous disease with complex tumor microenvironment (TME) alterations. However, immune cell signatures of TME and their prognostic value remain unclear in DLBCL. We aimed to identify high‐risk DLBCL with specific immune cell signatures in TME. Clinical and gene expression data of DLBCL patients were obtained from previously reported retrospective datasets in Gene Expression Omnibus (GSE108466 and GSE5378616) and a multi‐center prospective clinical trial NHL001 (NCT01852435). Patients treated with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) regimen (n = 159) from GSE10846 were referred as training cohort for CHOP regimen, while patients treated with rituximab‐CHOP (R‐CHOP) regimen (n = 192) from GSE10846 as training cohort for R‐CHOP regimen. Patients from NHL001 (n = 68) and GSE53786 (n = 57) were referred as validation cohorts for R‐CHOP regimen. CIBERSORT was applied to estimate the relative proportions of 22 subtype of immune cells. We established a prognostic model for model for R‐CHOP regimen included Age, performance status, lactate dehydrogenase, T cells follicular helper and macrophages M0, defining a low‐risk group with 2‐years OS of 92.9% and a high‐risk group with 2‐years OS of 52.5% (HR 6.57 [3.27–13.18], p < 0.0001). Immune cell signatures could be used as prognostic markers and provided further insights for individualized immunotherapeutic strategies in DLBCL.
Spatial modeling framework for aquatic exposure assessments of chemicals disposed down the drain: Case studies for China and Japan
Authors: McDonough Kathleen , Csiszar Susan A. , Fan Ming , Kapo Katherine , Menzies Jennifer , Vamshi Raghu 
Published: July 2021

Abstract
A modeling framework was created for the development of spatially explicit aquatic exposure models for any region or country of interest for chemicals disposed of down the drain. The framework relies on globally available data sets for river flow and population, and locally available data sets for wastewater treatment infrastructure and domestic water use, and leverages the iSTREEM® chemical routing algorithm. The framework was applied to China and Japan as case study countries. Spatially explicit population data were obtained from WorldPop. River flows covering the spatial extent of the two countries were derived from a high-resolution surface runoff gridded data set that was based on the Curve Number approach and combined with the hydrology network for catchments and rivers from HydroBASINS and HydroSHEDS data sets. Publicly available data from government sources were used for estimating per capita water use and wastewater treatment infrastructure. To demonstrate the framework, the China model was used to predict the levels of the antifungal agent climbazole in rivers across the country, and the Japan model was used to predict river concentrations of linear alkylbenzene sulfonate. For both chemicals, the comparison of measured to modeled values showed good agreement, using linear regression analysis (R2 ≥ 0.96). The framework presented in this study provides a systematic and robust approach to develop spatially resolved exposure models that can be extrapolated to any country or region, allowing more accurate risk assessment of chemicals disposed down the drain by leveraging concentration distributions generated by the model. Integr Environ Assess Manag 2022;18:722–733. © 2021 SETAC
KEY POINTS
Modeling framework created for the development of spatially explicit aquatic exposure models for any region or country of interest for chemicals disposed of down the drain. Framework relies on the use of a high-resolution global flow dataset developed as part of this research. Framework also relies on globally available datasets for population and locally available datasets for wastewater treatment infrastructure and domestic water use. Model framework was successfully applied to two case study countries, China and Japan, which differ significantly in population density, river flow (due to climate and geography), and wastewater treatment infrastructure.
Early detection of duodenal cancer by upper gastrointestinal‐endoscopy in Lynch syndrome
Authors: Vangala Deepak B. , Ladigan‐Badura Swetlana , Engel Christoph , Hüneburg Robert , Perne Claudia , Bucksch Karolin , Nattermann Jacob , Steinke‐Lange Verena , Rahner Nils , Weitz Jürgen , Kloor Matthias , Tomann Judith , Canbay Ali , Nguyen Huu‐Phuc , Strassburg Christian , Möslein Gabriele , Morak Monika , Holinski‐Feder Elke , Büttner Reinhard , Aretz Stefan , Löffler Markus , Schmiegel Wolff , Pox Christian , Schulmann Karsten ,   German Consortium for Familial Intestinal Cancer
Published: July 2021

AbstractSmall bowel cancer (SBC) is the malignancy with the highest standardized incidence ratio in Lynch syndrome (LS) patients. Of all SBCs, about 50% are duodenal cancers (DCs), therefore being accessible by esophago‐gastro‐duodenoscopy (EGD) for surveillance. We asked whether early detection of DC is possible for LS patients undergoing surveillance by EGD and if surveillance should be limited to specific subgroups. Data for LS patients with DC were retrieved from the registry of the German Consortium for Familial Intestinal Cancer. Patients undergoing active surveillance by EGDs (surveillance group) were compared to those who did not (nonsurveillance group) regarding tumor stage at diagnosis. Union for International Cancer Control stages I‐IIA were defined as early stage disease and IIB‐IV as advanced stage disease. Statistical analysis was performed using Fisher's exact test. Among 2015 patients with pathogenic variants in any mismatch‐repair‐gene, 47 patients with 49 DCs were identified. In 10% of cases, patients were under 35 years at diagnosis; family and personal tumor history did not correlate with DC diagnosis. Pathogenic germline variants in MSH6, PMS2 or EPCAM were present in 10% of patients. Statistical analysis could be performed on 13 DC patients in the surveillance group and 14 in the nonsurveillance group. Early detection was possible for 71% of patients in the surveillance group and 29% of patients in the nonsurveillance group (P = .021). Early detection of DC by EGD in LS patients is feasible regardless of family history, mutational status and should start no later than 25 years of age.
Definitive evidence for Club cells as progenitors for mutant Kras/Trp53‐deficient lung cancer
Authors: Rosigkeit Sebastian , Kruchem Marie , Thies Dorothe , Kreft Andreas , Eichler Emma , Boegel Sebastian , Jansky Sandrine , Siegl Dominik , Kaps Leonard , Pickert Geethanjali , Haehnel Patricia , Kindler Thomas , Hartwig Udo F. , Guerra Carmen , Barbacid Mariano , Schuppan Detlef , Bockamp Ernesto 
Published: July 2021

AbstractAccumulating evidence suggests that both the nature of oncogenic lesions and the cell‐of‐origin can strongly influence cancer histopathology, tumor aggressiveness and response to therapy. Although oncogenic Kras expression and loss of Trp53 tumor suppressor gene function have been demonstrated to initiate murine lung adenocarcinomas (LUADs) in alveolar type II (AT2) cells, clear evidence that Club cells, representing the second major subset of lung epithelial cells, can also act as cells‐of‐origin for LUAD is lacking. Equally, the exact anatomic location of Club cells that are susceptible to Kras transformation and the resulting tumor histotype remains to be established. Here, we provide definitive evidence for Club cells as progenitors for LUAD. Using in vivo lineage tracing, we find that a subset of Kras12V‐expressing and Trp53‐deficient Club cells act as precursors for LUAD and we define the stepwise trajectory of Club cell‐initiated tumors leading to lineage marker conversion and aggressive LUAD. Our results establish Club cells as cells‐of‐origin for LUAD and demonstrate that Club cell‐initiated tumors have the potential to develop aggressive LUAD.
Reducing risk for gestational diabetes among American Indian and Alaska Native teenagers: Tribal leaders' recommendations
Authors: Moore Kelly R. , Stotz Sarah , Abujaradeh Hiba , Marshall Gale , Terry Martha A. , Charron‐Prochownik Denise ,   for the Stopping GDM Study Group
Published: July 2021

AbstractObjectiveTo elicit feedback from tribal leaders and American Indian/Alaska Native (AI/AN) health system administrators as a national stakeholder perspective to inform the development of a gestational diabetes mellitus (GDM) risk reduction and preconception counseling intervention for AI/AN teenagers at high risk for GDM.MethodsA semi‐structured focus group interview guide was developed by both principal investigators and qualitative methods experts. Using open‐ended questions about the Reproductive‐health Education and Awareness of Diabetes in Youth for Girls (READY‐Girls) booklet and video clips, AI/AN health care system administrators and elected tribal leaders attending the 2015 National Indian Health Board Conference in Washington, DC, made recommendations on adaptation for an AI/AN audience. The focus group was recorded, transcribed verbatim, and analyzed by two researchers using an inductive coding technique with constant comparison method as supported by the grounded theory approach.ResultsRecommendations from the 12 participants included: (1) the best ways to communicate with AI/AN teenagers, (2) the importance of parental, family, and community education and engagement to support AI/AN teenagers in GDM risk reduction, and (3) building on traditional AI/AN cultural values and practices, while accommodating differences between tribes and regions.ConclusionFindings from this focus group were used to inform the iterative development of a GDM risk reduction and preconception counseling intervention for AI/AN teenagers.
Nonlocal convolutional block attention module VNet for gliomas automatic segmentation
Authors: Fang Ying , Huang He , Yang Weiji , Xu Xiaomei , Jiang Weiwei , Lai Xiaobo 
Published: July 2021

AbstractGlioma is the most common primary tumor in the skull, but it has no obvious boundary with normal brain tissue and is difficult to completely remove. Currently, manual segmentation of the lesion regions has been widely used in the clinical practice of magnetic resonance (MR) images of gliomas, but the implementation process has disadvantages such as time‐consuming and poor repeatability. It is because of the shortcomings of traditional segmentation methods that we must seek other efficient technical means, which promote the development of automatic image segmentation technology. In this study, we propose a glioma automatic segmentation method called NLCA‐VNet. The framework is based on VNet, adding nonlocal and convolutional block attention modules, which can maintain more information, and can carry out attention in the channel and spatial dimensions, so that improve the segmentation effect. We employ the extended glioma MR image data set by the Brain Tumor Segmentation Challenge database (BraTS 2020, 2019, 2018), and finally obtained the effect image after tumor segmentation and achieved average Dice scores of 0.6702, 0.876, 0.7687, sensitivity of 0.7494, 0.9209, 0.7702, specificity of 0.0.9994, 0.9985, 0.9995, and Hausdorff95 of 50.8613, 9.3667, 12.4573 for enhancing tumor core, whole tumor, and tumor core in BraTS 2020, respectively. The results fully show that our method can fully adapt to the segmentation of glioma. To a certain extent, it improves the efficiency and accuracy of the doctor's diagnosis, which is of great significance to the scientific research and clinical aspects of glioma.
Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier
Authors: Király Nikolett , Thalwieser Zsófia , Fonódi Márton , Csortos Csilla , Boratkó Anita 
Published: July 2021

AbstractAnnexin A2 (ANXA2) is a multifunctional protein expressed in nearly all human tissues and cell types, playing a role in various signaling pathways. It is subjected to phosphorylation, but no specific protein phosphatase has been identified in its posttranslational regulation yet. Using pull‐down assay followed by liquid chromatography–mass spectrometry analysis we found that ANXA2 interacts with TIMAP (TGF‐beta‐inhibited membrane‐associated protein) in pulmonary artery endothelial cells. TIMAP is highly expressed in endothelial cells, where it acts as a regulatory and targeting subunit of protein phosphatase 1 (PP1). TIMAP plays an important role in the regulation of the endothelial barrier maintenance through the dephosphorylation of its several substrate proteins. In the present work, phosphorylation of Ser25 side chain in ANXA2 by protein kinase C (PKC) was shown both in vivo and in vitro. Phosphorylation level of ANXA2 at Ser25 increased greatly by inhibition of PP1 and by depletion of its regulatory subunit, TIMAP, implying a role of this PP1 holoenzyme in the dephosphorylation of ANXA2. Immunofluorescence staining and subcellular fractionations revealed a diffuse subcellular localization for the endogenous ANXA2, but phospho‐Ser25 ANXA2 was mainly detected in the membrane. ANXA2 depletion lowered the basal endothelial barrier and inhibited cell migration, but had no significant effect on cell proliferation or viability. ANXA2 depleted cells failed to respond to PMA treatment, indicating an intimately involvement of phospho‐ANXA2 in PKC signaling. Moreover, phosphorylation of ANXA2 disrupted its interaction with S100A10 suggesting a phosphorylation dependent multiple regulatory role of ANXA2 in endothelial cells. Our results demonstrate the pivotal role of PKC‐ANXA2‐PP1 pathway in endothelial cell signaling, especially in barrier function and cell migration.
Near‐infrared emissive polymer‐coated IR‐820 nanoparticles assisted photothermal therapy for cervical cancer cells
Authors: Zhu Lijun , Chen Jianjun , Yan Ting , Alimu Gulinigaer , Zhang Xueliang , Chen Shuang , Aimaiti Mutalifu , Ma Rong , Alifu Nuernisha 
Published: July 2021

AbstractPhotothermal therapy (PTT) has attracted wide attention due to its noninvasiveness and its thermal ablation ability. As photothermal agents are crucial factor in PTT, those with the characteristics of biocompatibility, non‐toxicity and high photothermal stability have attracted great interest. In this work, new indocyanine green (IR‐820) was utilized as a photothermal agent and near‐infrared (NIR) fluorescence imaging nanoprobe. To improve the biocompatibility, poly(styrene‐co‐maleic anhydride) (PSMA) was utilized to encapsulate the IR‐820 molecules to form novel IR‐820@PSMA nanoparticles (NPs). Then, the optical and thermal properties of IR‐820@PSMA NPs were studied in detail. The IR‐820@PSMA NPs showed excellent photothermal stability and biocompatibility. The cellular uptaking ability of the IR‐820@PSMA NPs was further confirmed in HeLa cells by the NIR fluorescent confocal microscopic imaging technique. The IR‐820@PSMA NPs assisted PTT of living HeLa cells was conducted under 793 nm laser excitation, and a high PTT efficiency of 73.3% was obtained.
Chitosan/hydroxyapatite nanocomposite scaffolds to modulate osteogenic and inflammatory response
Authors: Soriente Alessandra , Fasolino Ines , Gomez‐Sánchez Alejandro , Prokhorov Evgen , Buonocore Giovanna Giuliana , Luna‐Barcenas Gabriel , Ambrosio Luigi , Raucci Maria Grazia 
Published: July 2021

ABSTRACTConsiderable attention has been given to the use of chitosan (CS)‐based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss. It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property. Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE). The combination of sol–gel approach and freeze‐drying technology was used to obtain CS/HA scaffolds with three‐dimensional (3D) porous structure suitable for cell in‐growth. Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti‐inflammatory effects for treating bone defects. The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype. Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro‐inflammatory (TGF‐β) and anti‐inflammatory (IL‐4, IL‐10) cytokines. Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal. Contemporarily, these materials allow avoiding the induction of a pro‐inflammatory response in bone implant site.
Pooled analysis of LAMP assay for the diagnosis of norovirus infection
Authors: Qian Xi‐Feng , Duan Ai‐Ling , Huang Rong‐Xian , Li Nan‐Xi , Li Ya‐Ping , Guo Xu‐Guang 
Published: July 2021

AbstractBackgroundRapid laboratory detection is essential to diagnose norovirus infection. LAMP has many advantages compared with RT‐PCR for detecting norovirus, including high sensitivity, high specificity, rapidity, low cost, and intuitive results, which can be easily read with the naked eye with the help of color‐based reporters. In this study, we intend to analyze the accuracy of LAMP methods for the diagnosis of norovirus infection.MethodsTwo researchers independently retrieved relevant literature up to January 2021 (PubMed, Web of Science, Cochrane Library, Embase, CNKI, Wan Fang, and VIP). The researchers screened all articles and extracted their research data for meta‐analysis. QUADAS‐2 tool was used to evaluate the quality of the included studies by Review Manager 5.3. Forest plots were performed by Meta‐DiSc 1.4 to evaluate the accuracy of the test. Deeks’ funnel plot symmetry tests were conducted by Stata 15.0 to check the potential publication bias.ResultsEleven sets of data extracted from the eight included studies were included for meta‐analysis. For the detection of norovirus, the pooled sensitivity, specificity, positive LR, negative LR, diagnostic OR, and their 95% CI were 0.96 (0.95–0.97), 0.99 (0.99–1.00), 91.14 (31.88–260.56), 0.06 (0.04–0.09), and 1473.68 (562.96–3857.70), respectively. Besides, AUC in the SROC curve was 0.9920.ConclusionLAMP had high sensitivity and specificity in terms of the diagnosis of norovirus infection. However, further extension of this approach should be researched to ensure the accuracy and practicability of this hopeful test in the future.
CircRNAs as promising biomarker in diagnosis of breast cancer: An updated meta‐analysis
Authors: Chu Mingyu , Fang Yaqun , Jin Yucui 
Published: July 2021

AbstractBackgroundCircular RNAs (circRNAs) have been identified to be involved in onset and progression of multiple malignant tumors. The present study aimed to systematically evaluate the diagnostic values of circRNAs in breast cancer.MethodsThe PubMed, Web of Science, Embase, CNKI, and Wanfang online databases were searched for the relevant studies before December 31, 2020. Statistical analysis of the diagnostic tests was performed based on STATA 16.0, Meta‐DiSc 1.4, and RevMan 5.3 software. The threshold effect and publication bias were measured by the Spearman correlation and Deeks’ funnel plot asymmetry test, respectively.ResultsTwenty‐one studies from 13 articles were included in this meta‐analysis. The pooled sensitivity and specificity were 0.77 and 0.71, respectively. The pooled positive likelihood ratio (PLR), negative likelihood ratio (NLR), and overall diagnostic odds ratio (DOR) were 2.6, 0.33, and 8, respectively. Furthermore, the area under the summary receiver operator characteristic curve was 0.80. In addition, down‐regulated circRNAs achieved a diagnostic performance higher than up‐regulated circRNAs, with area under curve (AUC) values of 0.81 and 0.74, respectively. Studies based on tissue samples presented better diagnostic accuracy than those based on plasma samples, with AUC values of 0.80 and 0.67. In addition, two circRNAs, including circ_0001073 and circTADA2A‐E5/E6, showed higher diagnostic values, with AUC value of 0.990 and 0.937, respectively. According to the results of meta‐regression, the case size (p<0.05) might be the source of the heterogeneity.ConclusionCircRNAs exhibited a high diagnostic value for breast cancer and may function as potential diagnostic biomarkers for breast cancer.
Characterization of anatomical foliar epidermal features of herbaceous flora of Tilla Jogian, Pakistan by using light microscopy techniques
Authors: Shahzad Kanwal , Zafar Muhammad , Khan Amir Muhammad , Mahmood Tariq , Abbas Qamar , Ozdemir Fethi Ahmet , Ahmad Mushtaq , Sultana Shazia 
Published: July 2021

AbstractIn current study, 22 herbaceous plant specimens have been examined with the help of light microscope to carry out leaf epidermal anatomy. Foliar micro‐morphology of herbaceous plants from Tilla Jogian, Jhelum Pakistan was held first time. The plants were collected from different parts of Tilla Jogian at different elevations. The main objective of the study is to use this information as an aid in plant taxonomy. Significant variations were observed in both qualitative and quantitative features. With the help of microscopy significant variations in characters like stomata size, number, epidermal cell size, shape and number, guard cell size, trichomes, and subsidiary cells were recorded. On the basis of these findings, light microscopy of leaf epidermal anatomical features holds great interest for taxonomists to identify complete taxa. Foliar epidermal anatomy of herbaceous flora serves as baseline data along with taxonomical data and can be helpful in demarcation and limitation of nearly associated taxa to gain better use of anatomical characters.
Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans
Authors: de Andrade Pereira Lorrane , de Carvalho Queiroz Margareth Maria , Faria Suzana Côrte‐Real , Zahner Viviane 
Published: July 2021

AbstractBrevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain‐specific molecules. In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical‐veterinary and sanitary importance, and synanthropy. The enormous biotechnological potential of B. laterosporus has been demonstrated, but there are still few studies with muscoid dipterans. The aim of the study was to verify the mortality of B. laterosporus NRS590 on synanthropic flies and to characterize its different cell stages ultrastructurally. The flies were collected from garbage bins and the colonies were adapted to the laboratory conditions. Bioassays with neo larvae were carried out from the bacterial growth in the phases: vegetative (6 hr), sporangium (20 hr), and free spores (44 hr). An aliquot of each phase was collected for Transmission (TEM) and Scanning Electron Microscopy (SEM). The effectiveness of NRS590 was observed in the sporulation phase, where the corrected mortality was 83.3, 85.1, and 99% for Chrysomya megacephala, Chrysomya putoria, and Musca domestica, respectively. The parasporal body was observed in detail on the entire spore surface. Although our knowledge of this bacterium is growing, it remains to be determined the real virulence factors responsible for the wide entomopathogenic activity observed on muscoid dipterans. Therefore, this study can provide subsidies for the improvement of efficient and safe microbial control techniques for the environment and living beings.