bluuebunny/crossref_metadata_2025_split huggingface dataset:
RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway
Authors: Hou Jianbing , Liu Yudong , Huang Pan , Wang Yutao , Pei Dakun , Tan Ruoyue , Zhang Yundong , Cui Hongjuan 
Published: October 2021

AbstractRAN binding protein 10 (RANBP10), a ubiquitously expressed and evolutionarily conserved protein, as a RAN-GTP exchange factor (GEF) to regulate several factors involved in cellular progression. Previous studies showed that RANBP10 was overexpressed in prostate cancer cells and was responsible for androgen receptor (AR) activation. However, the biological function of RANBP10 in glioblastoma (GBM) has not been studied. Here, we found that RANBP10 was overexpressed in GBM, and high RANBP10 expression was closely linked to poor survival of patients with GBM. Downregulation of RANBP10 significantly inhibited cell proliferation, migration, invasion, and tumor growth of GBM cells. In addition, we revealed that RANBP10 could suppress the promoter activity of FBXW7, and thereby increase the protein stability of c-Myc in GBM cells. Silencing of FBXW7 in RANBP10-knockdown GBM cells could partly negate the effects induced by RANBP10 downregulation. Taken together, our findings established that RANBP10 significantly promoted GBM progression by control of the FBXW7–c-Myc axis, and suggest that RANBP10 may be a potential target in GBM.
Identification of histone methyltransferase NSD2 as an important oncogenic gene in colorectal cancer
Authors: Zhao Li-hao , Li Quan , Huang Zhi-Jun , Sun Mi-Xue , Lu Jing-jing , Zhang Xiao-hua , Li Gang , Wu Fang 
Published: October 2021

AbstractColorectal cancer (CRC) is the second common cause of cancer-related human mortalities. Dysregulation of histone 3 (H3) methylation could lead to transcriptional activation of multiple oncogenes, which is closely associated with CRC tumorigenesis and progression. Nuclear receptor-binding SET Domain protein 2 (NSD2) is a key histone methyltransferase catalyzing histone H3 lysine 36 dimethylation (H3K36me2). Its expression, the potential functions, and molecular mechanisms in CRC are studied here. Gene Expression Profiling Interactive Analysis (GEPIA) bioinformatics results showed that the NSD2 mRNA expression is elevated in both colon cancers and rectal cancers. Furthermore, NSD2 mRNA and protein expression levels in local colon cancer tissues are significantly higher than those in matched surrounding normal tissues. In primary human colon cancer cells and established CRC cell lines, shRNA-induced silencing or CRISPR/Cas9-induced knockout of NSD2 inhibited cell viability, proliferation, cell cycle progression, migration, and invasion. Furthermore, NSD2 shRNA or knockout induced mitochondrial depolarization, DNA damage, and apoptosis in the primary and established CRC cells. Contrarily, ectopic NSD2 overexpression in primary colon cancer cells further enhanced cell proliferation, migration, and invasion. H3K36me2, expressions of multiple oncogenes (ADAM9, EGFR, Sox2, Bcl-2, SYK, and MET) and Akt activation were significantly decreased after NSD2 silencing or knockout in primary colon cancer cells. Their levels were however increased after ectopic NSD2 overexpression. A catalytic inactive NSD2 (Y1179A) also inhibited H3K36me2, multiple oncogenes expression, and Akt activation, as well as cell proliferation and migration in primary colon cancer cells. In vivo, intratumoral injection of adeno-associated virus (AAV)-packed NSD2 shRNA largely inhibited primary colon cancer cell xenograft growth in nude mice. Together, NSD2 exerted oncogenic functions in CRC and could be a promising therapeutic target.
Intertwined ferroelectricity and topological state in two-dimensional multilayer
Authors: Liang Yan , Mao Ning , Dai Ying , Kou Liangzhi , Huang Baibiao , Ma Yandong 
Published: October 2021

AbstractThe intertwined ferroelectricity and band topology will enable the non-volatile control of the topological states, which is of importance for nanoelectrics with low energy costing and high response speed. Nonetheless, the principle to design such system is unclear and the feasible approach to achieve the coexistence of two parameter orders is absent. Here, we propose a general paradigm to design 2D ferroelectric topological insulators by sliding topological multilayers on the basis of first-principles calculations. Taking trilayer Bi2Te3 as a model system, we show that in the van der Waals multilayer based 2D topological insulators, the in-plane and out-of-plane ferroelectricity can be induced through a specific interlayer sliding, to enable the coexistence of ferroelectric and topological orders. The strong coupling of the order parameters renders the topological states sensitive to polarization flip, realizing non-volatile ferroelectric control of topological properties. The revealed design-guideline and ferroelectric-topological coupling not only are useful for the fundamental research of the coupled ferroelectric and topological physics in 2D lattices, but also enable innovative applications in nanodevices.
Hybrid immunity improves B cells and antibodies against SARS-CoV-2 variants
Authors: Andreano Emanuele , Paciello Ida , Piccini Giulia , Manganaro Noemi , Pileri Piero , Hyseni Inesa , Leonardi Margherita , Pantano Elisa , Abbiento Valentina , Benincasa Linda , Giglioli Ginevra , De Santi Concetta , Fabbiani Massimiliano , Rancan Ilaria , Tumbarello Mario , Montagnani Francesca , Sala Claudia , Montomoli Emanuele , Rappuoli Rino 
Published: October 2021

AbstractThe emergence of SARS-CoV-2 variants is jeopardizing the effectiveness of current vaccines and limiting the application of monoclonal antibody-based therapy for COVID-19 (refs. 1,2). Here we analysed the memory B cells of five naive and five convalescent people vaccinated with the BNT162b2 mRNA vaccine to investigate the nature of the B cell and antibody response at the single-cell level. Almost 6,000 cells were sorted, over 3,000 cells produced monoclonal antibodies against the spike protein and more than 400 cells neutralized the original SARS-CoV-2 virus first identified in Wuhan, China. The B.1.351 (Beta) and B.1.1.248 (Gamma) variants escaped almost 70% of these antibodies, while a much smaller portion was impacted by the B.1.1.7 (Alpha) and B.1.617.2 (Delta) variants. The overall loss of neutralization was always significantly higher in the antibodies from naive people. In part, this was due to the IGHV2-5;IGHJ4-1 germline, which was found only in people who were convalescent and generated potent and broadly neutralizing antibodies. Our data suggest that people who are seropositive following infection or primary vaccination will produce antibodies with increased potency and breadth and will be able to better control emerging SARS-CoV-2 variants.
Transparent, self-cleaning, scratch resistance and environment friendly coatings for glass substrate and their potential applications in outdoor and automobile industry
Authors: Shahzadi Phool , Gilani Syeda Rubina , Rana Bakht Bahadur , Ghaffar Abdul , Munir Akhtar 
Published: October 2021

AbstractIn this research work six novel combinations of Hydroxy Ethyl Meth Acrylate based copolymers have been synthesized and commercial titania, after activation was added by adopting simple strategy to manufacture super-hydrophobic, cost effective, transparent, antifogging, self-cleaning and antimicrobial coating on the glass sheet which will be helpful for outdoor and automobile windscreen. The super-hydrophobic covering was set up by dip covering procedure and coated specimen have been characterized for Wetting behaviour, transparency and SEM analysis. Likewise, the dependability of the coating was evaluated at conditions comparable strengthening at higher temperatures (4–400 °C), illumination by UV spectrum at basic and acidic limits, Results revealed that developed material has good adhesion with glass and shows transparency more than 97%, and water contact edge (CA) of 135 ± 2°. Furthermore, the covering displays astounding self-cleaning property. All the outcomes demonstrated that such kind of coatings could be used many modern level applications on automobile wind screen and glass-windows in building and other glasses where protection from UV radiation, anti-fogging and cleaning is required. Such type of coating material can also be used to preserve architectural work leather and other decoration and artwork. The graphical representation is given in Fig. 1.
Mathematical epidemiologic and simulation modelling of first wave COVID-19 in Malaysia
Authors: Ariffin Muhammad Rezal Kamel , Gopal Kathiresan , Krishnarajah Isthrinayagy , Che Ilias Iszuanie Syafidza , Adam Mohd Bakri , Arasan Jayanthi , Abd Rahman Nur Haizum , Mohd Dom Nur Sumirah , Mohammad Sham Noraishah 
Published: October 2021

AbstractSince the first coronavirus disease 2019 (COVID-19) outbreak appeared in Wuhan, mainland China on December 31, 2019, the geographical spread of the epidemic was swift. Malaysia is one of the countries that were hit substantially by the outbreak, particularly in the second wave. This study aims to simulate the infectious trend and trajectory of COVID-19 to understand the severity of the disease and determine the approximate number of days required for the trend to decline. The number of confirmed positive infectious cases [as reported by Ministry of Health, Malaysia (MOH)] were used from January 25, 2020 to March 31, 2020. This study simulated the infectious count for the same duration to assess the predictive capability of the Susceptible-Infectious-Recovered (SIR) model. The same model was used to project the simulation trajectory of confirmed positive infectious cases for 80 days from the beginning of the outbreak and extended the trajectory for another 30 days to obtain an overall picture of the severity of the disease in Malaysia. The transmission rate, β also been utilized to predict the cumulative number of infectious individuals. Using the SIR model, the simulated infectious cases count obtained was not far from the actual count. The simulated trend was able to mimic the actual count and capture the actual spikes approximately. The infectious trajectory simulation for 80 days and the extended trajectory for 110 days depicts that the inclining trend has peaked and ended and will decline towards late April 2020. Furthermore, the predicted cumulative number of infectious individuals tallies with the preparations undertaken by the MOH. The simulation indicates the severity of COVID-19 disease in Malaysia, suggesting a peak of infectiousness in mid-March 2020 and a probable decline in late April 2020. Overall, the study findings indicate that outbreak control measures such as the Movement Control Order (MCO), social distancing and increased hygienic awareness is needed to control the transmission of the outbreak in Malaysia.
Application of long short-term memory neural network technique for predicting monthly pan evaporation
Authors: Abed Mustafa , Imteaz Monzur Alam , Ahmed Ali Najah , Huang Yuk Feng 
Published: October 2021

AbstractEvaporation is a key element for water resource management, hydrological modelling, and irrigation system designing. Monthly evaporation (Ep) was projected by deploying three machine learning (ML) models included Extreme Gradient Boosting, ElasticNet Linear Regression, and Long Short-Term Memory; and two empirical techniques namely Stephens-Stewart and Thornthwaite. The aim of this study is to develop a reliable generalised model to predict evaporation throughout Malaysia. In this context, monthly meteorological statistics from two weather stations in Malaysia were utilised for training and testing the models on the basis of climatic aspects such as maximum temperature, mean temperature, minimum temperature, wind speed, relative humidity, and solar radiation for the period of 2000–2019. For every approach, multiple models were formulated by utilising various combinations of input parameters and other model factors. The performance of models was assessed by utilising standard statistical measures. The outcomes indicated that the three machine learning models formulated outclassed empirical models and could considerably enhance the precision of monthly Ep estimate even with the same combinations of inputs. In addition, the performance assessment showed that Long Short-Term Memory Neural Network (LSTM) offered the most precise monthly Ep estimations from all the studied models for both stations. The LSTM-10 model performance measures were (R2 = 0.970, MAE = 0.135, MSE = 0.027, RMSE = 0.166, RAE = 0.173, RSE = 0.029) for Alor Setar and (R2 = 0.986, MAE = 0.058, MSE = 0.005, RMSE = 0.074, RAE = 0.120, RSE = 0.013) for Kota Bharu.
Metabolic derangement in polycystic kidney disease mouse models is ameliorated by mitochondrial-targeted antioxidants
Authors: Daneshgar Nastaran , Baguley Andrew W. , Liang Peir-In , Wu Fei , Chu Yi , Kinter Michael T. , Benavides Gloria A. , Johnson Michelle S. , Darley-Usmar Victor , Zhang Jianhua , Chan Kung-Sik , Dai Dao-Fu 
Published: October 2021

AbstractAutosomal dominant polycystic kidney disease (ADPKD) is characterized by progressively enlarging cysts. Here we elucidate the interplay between oxidative stress, mitochondrial dysfunction, and metabolic derangement using two mouse models of PKD1 mutation, PKD1RC/nulland PKD1RC/RC. Mouse kidneys with PKD1 mutation have decreased mitochondrial complexes activity. Targeted proteomics analysis shows a significant decrease in proteins involved in the TCA cycle, fatty acid oxidation (FAO), respiratory complexes, and endogenous antioxidants. Overexpressing mitochondrial-targeted catalase (mCAT) using adeno-associated virus reduces mitochondrial ROS, oxidative damage, ameliorates the progression of PKD and partially restores expression of proteins involved in FAO and the TCA cycle. In human ADPKD cells, inducing mitochondrial ROS increased ERK1/2 phosphorylation and decreased AMPK phosphorylation, whereas the converse was observed with increased scavenging of ROS in the mitochondria. Treatment with the mitochondrial protective peptide, SS31, recapitulates the beneficial effects of mCAT, supporting its potential application as a novel therapeutic for ADPKD.
Addressing Emotional and Psychological Problems Associated With Hearing Loss: Perspective of Consumer and Community Representatives
Authors: Bennett Rebecca J. , Donaldson Sara , Kelsall-Foreman India , Meyer Carly , Pachana Nancy A. , Saulsman Lisa , Eikelboom Robert H. , Bucks Romola S. 
Published: October 2021

Purpose:
Hearing loss causes emotional distress and can contribute to the development of psychological difficulties, yet emotional and psychological issues are not addressed within current audiology services. The purpose of this study was to use focus groups with consumer and community representatives to explore how we might improve the provision of support for clients experiencing emotional and psychological issues in relation to their hearing loss.

Participants:

Adults with hearing loss (
n
= 19) and their significant others (
n
= 9), as well as 10 hearing health care professionals (
n
= 4 hearing health care clinicians,
n
= 4 reception staff, and
n
= 2 clinical managers), participated in consumer and community engagement focus groups.

Method:
Consumer and community representatives were tasked with (a) identifying the stakeholders involved in supporting adults experiencing emotional or psychological difficulties on account of their hearing loss, (b) describing the behaviors undertaken by each stakeholder group, and (c) selecting target behavior(s) that could optimally form the basis of an intervention program to improve the quality and frequency of support provided to people experiencing emotional and psychological problems in the audiology setting.

Results:
Participants identified 12 stakeholder groups involved in supporting adults with hearing loss experiencing emotional and psychological problems. The three behaviors voted by participants to be the most promising for a behavioral intervention included the clinician (a) asking about, (b) providing information on, and (c) delivering therapeutic intervention for emotional and psychological well-being within audiological service provision.

Conclusion:
Consumer and community stakeholder representatives indicate a general desire for hearing health care clinicians to deliver support for the emotional and psychological issues that arise relating to hearing loss.
Sprunggelenk- und Vorfußtherapie in der Rheumatologie
– wie lange können wir den aufrechten Gang
erhalten?
Authors: Gaulke Ralph 
Published: October 2021

ZusammenfassungDie Therapie des rheumatischen Fußes ist komplex und erstreckt sich
über ein breites Spektrum von konservativen und operativen
Behandlungsmethoden. Das Ziel ist, bei guter Stabilität,
möglichst viel Beweglichkeit zu erhalten. Nebenerkrankungen wie die
Polyneuropathie mit und ohne Diabetes mellitus beeinflussen die
Behandlungsoptionen entscheidend. Aufgrund dessen, dass beim Rheumatiker
multiple Gelenke befallen sind, unterliegen die orthopädische
Schuhversorgung und die operative Therapie des rheumatischen Fußes
speziellen Anforderungen im Hinblick auf das Gewicht, die Stabilität des
Schuhs und Bettung des Fußes bzw. die Zugangswege und die
Primärstabilität der Osteosynthesen. Der aufrechte Gang kann
durch einen stadiengerechten zielgerichteten Einsatz aller Therapieoption sehr
lange erhalten bleiben. Hierfür müssen die therapeutischen
Fenster erkannt und genutzt sowie eine Übertherapie vermieden
werden.
Funktionelle Charakterisierung von Patienten mit isoliert postkapillärer oder kombiniert post- und präkapillärer pulmonaler Hypertonie
Authors: Held Matthias , Weiner Simon , Walthelm Johanna , Joa Franziska , Hoffmann Jörg , Güder Gülmisal , Pfeuffer-Jovic Elena 
Published: October 2021

Zusammenfassung
Hintergrund Nach Empfehlungen der letzten PH-Weltkonferenz wird die Differenzierung von isoliert postkapillärer (Ipc) und kombiniert post- und präkapillärer (Cpc) PH allein nach pulmonal-vaskulärem Widerstand (PVR) empfohlen. Ziel dieser Arbeit war es, Patienten mit IpcPH und CpcPH nach den aktualisierten Empfehlungen basierend auf der letzten Weltkonferenz für pulmonale Hypertonie (PH) hämodynamisch und im Rahmen einer explorativen Datenanalyse funktionell zu charakterisieren.
Methoden Evaluiert wurden Patienten, die sich von 2008 bis 2015 in der PH-Ambulanz der Missioklinik Würzburg mit Verdacht auf PH zur leitlinienkonformen Diagnostik vorstellten. Analysiert wurden alle Patienten mit einem pulmonal-arteriellen Mitteldruck (mPAP) von ≥ 25 mmHg und einem pulmonalkapillären Verschlussdruck (PCWP) > 15 mmHg. Es erfolgte der Vergleich anthropometrischer, hämodynamischer und funktioneller Daten aus 6-Minuten-Gehtest (6MWT), Spiroergometrie und Echokardiografie bei IpcPH und CpcPH.
Ergebnisse Von 726 Patienten zeigten 58 eine postkapilläre PH: IpcPH: n = 20; CpcPH: n = 38. Patienten mit IpcPH besaßen einen signifikant niedrigeren mPAP und PVR als Patienten mit CpcPH. Der Herzindex war in der CpcPH-Gruppe niedriger als in der IpcPH-Gruppe. Die funktionelle Kapazität beider Gruppen war nicht verschieden. Patienten mit CpcPH hatten einen größeren rechts-linksatrialen Flächen(RA/LA)-Quotienten.
Diskussion und Schlussfolgerung Trotz höherem mPAP und PVR von Patienten mit CpcPH findet sich keine schlechtere funktionelle Kapazität als bei IpcPH. Bei Patienten mit Linksherzerkrankungen und PH kann ein erhöhter RA/LA-Index ein möglicher Hinweis für eine CpcPH sein und Anlass zur invasiven Diagnostik geben.
Examination of common swifts (Apus apus) for salmonella shedding in the area of Hannover, Lower Saxony, Germany
Authors: Tiyawattanaroj Warakorn , Jung Arne , Mohr Lydia , Legler Marko 
Published: October 2021

AbstractObjective The available literature indicates a high prevalence of the zoonotic pathogen Salmonella (S.) enterica serovar Infantis in the common swift (Apus apus). This long-distance migrant, which only consumes aerial plankton, can reach high population densities in places with suitable breeding sites. Dedicated competent private persons take part in the hand rearing of juvenile common swifts in wildlife rescue centres, which unavoidably results in close contact with these avian patients. For this reason, we examined common swifts for shedding of Salmonella spp.Material and methods In the years 2014 and 2019, intestinal swabs or fresh faeces of common swifts (2014: n = 54; 2019: n = 62) were examined microbiologically (DIN EN ISO 6579; Annex D) in the area of Hannover, Lower Saxony, Germany.Results Salmonella spp. could not be detected in any of the examined common swifts within the investigation period and the studied area in 2014 and 2019.Conclusion and clinical relevance The results illustrate that the common swift is unlikely to be a natural reservoir of Salmonella spp. For the transmission of salmonella by swifts the local conditions with the corresponding environmental impact seem to play a significant role, and the risk of transmission should be assessed according to the region to be examined.
Acceptance of SARS-CoV-2 vaccines by liver transplant recipients and candidates
Authors: Reuken Philipp A. , Albers Stefanie , Rauchfuss Falk , Strnad Pavel , Settmacher Utz , Trautwein Christian , Stallmach Andreas , Bruns Tony 
Published: October 2021

AbstractGuidelines recommend vaccination against SARS-CoV-2 in transplant recipients, candidates, and their household contacts. However, little is known about the acceptance of COVID-19 vaccines in these groups.In March 2021, we surveyed 826 liver transplant recipients, candidates, and their household contacts to determine acceptance rates and factors influencing the acceptance of the COVID-19 vaccine; 341 patients (40%) and 237 household contacts (28%) returned the questionnaire. Ninety percent of patients returning the survey reported they were willing to receive the vaccine within the next 6 months or had already started vaccination. Only 2% of patients and 4% of household contacts reported refusing the vaccine, and 8% of patients and 9% of household contacts wanted to postpone vaccination because of concerns about side effects. Having received the influenza vaccine in the last 2 seasons was the strongest indicator of acceptance to receive the SARS-CoV-2 vaccine within 6 months (odds ratio 5.11; 95% confidence interval 2.43–10.74; p < 0.001). Thirty-two percent of responding patients reported fear of side effects as a reason for having refused vaccination before.Although the acceptance of the SARS-CoV-2 vaccine was particularly high among German liver transplant recipients, candidates, and household contacts in this survey, transplant physicians are encouraged to discuss safety concerns with patients who have refused the seasonal influenza vaccine in the past.
Enantioselective C–H Alkylation of N-Arylbenzamides with Vinyl Ethers Catalyzed by an Iridium/Chiral Phosphoramidite–Olefin Complex
Authors: Nishimura Takahiro , Murakami Kotone , Sakamoto Kana 
Published: October 2021

AbstractChiral phosphoramidite–olefin hybrid ligands were found to be effective in the iridium-catalyzed asymmetric alkylation of N-arylbenzamides with vinyl ethers. The reaction is catalyzed by a hydroxo­iridium catalyst coordinated with the hybrid ligand to give the corresponding products in high yields with high branch selectivity and enantioselectivity.
C–H Amination of Nitro Azaheterocyclic Compounds by Vicarious Nucleophilic Substitution
Authors: Cai Chun , Zhou Ru-Shuang 
Published: October 2021

AbstractVarious nitro azaheterocyclic compounds were subjected to C–H amination by vicarious nucleophilic substitution with 4H-1,2,4-triazol-4-amine (ATA). The aminated products were characterized by NMR, mass spectroscopy, and single-crystal X-ray diffraction analyses. The substrates examined gave moderate to excellent yields (30–88%) and showed good regioselectivities. This protocol offers the advantages of mild conditions, a short reaction time (2–4 hours), and an inexpensive, commercially available, and less-toxic amination reagent; moreover, no additional catalyst or reagent is needed. A possible reaction mechanism is discussed.