bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Cinnamon and Turmeric Nanoparticles Extract on microorganisms of Fresh Ground Beef During Cold Storage
Authors: Al-Zubaidi Labeeb ahmed , Al-Rubeii Amera Mohammed , Al-Salmany Ahmed Sami 
Published: November 2021

Abstract
This study aimed to evaluate the effect of cinnamon and turmeric nanoscale extracts on reducing the microbial load of ground beef stored under refrigerated temperature at 2 ° C for 12 days. The study included six different treatments T1 positive control treatment (adding water), T2 negative control treatment (without addition) and T3. (Curcuma 248.3 ppm), T4 (Curcuma 496.9 ppm), T5 (Cinnamon 83.08 ppm) and T6 (Cinnamon 166.16 ppm). The treatments were kept with storage periods of 1, 4, 8 and 12 days, respectively. Some tests were performed to detect microorganisms. The results of the treatments recorded lower values for the total number of bacteria when compared with the two control treatments, and the treatment T6 recorded the lowest values. The results observed that there was a significant decrease (P< 0.05) in the numbers of cold-loving bacteria for the treatments to which the cinnamon and turmeric nanoparticles extract was added. Treatment T6 (cinnamon 166.16 ppm) recorded lowest number of cold-loving bacteria, reaching 4.47, 4.57 and 4.77, 5.22 bacterial units/gm. meat. The results of the added treatments showed a significant decrease (P <0.05) in the logarithm of the number of coliform bacteria compared to the positive control treatments (T1 and T2). In addition, T6 (cinnamon 166.16 ppm) reached lowest number of coliform bacteria and reached 3.69, 3.78, 3.87, 3.99 bacterial units/gm meat. This study concluded that the addition of cinnamon and turmeric nanoparticles extracts to fresh beef and stored by refrigerating for different storage periods. This antibacterial activity assured by a decrease in the logarithm of the total number of normal bacteria, cold-loving bacteria and coliform bacteria, compared with the control treatments for ground beef and cryogenic stock.
Water Quality and Conservation Status of Fish Fauna of Main Outfall Drain, Al-Diwaniya City, Iraq
Authors: Al-Zaidy Kadhim J.L. 
Published: November 2021

Abstract
Freshwater fish diversity, abundance, the ecological indices, and water quality from Main Outfall Drain (MOD) in Al-Diwaniya City/middle Iraq were studied monthly from January to December 2017 in two sites. The present study has shown that the third river (MOD) supported 15 fish species belonging to 7 families. As far as biodiversity status in fish community (IUCN-2019) is concerned, out of 15 species, eleven fish species are categorized into Lower risk least concern (LC or LR/lc), four Vulnerable (VU). Eight species were sensitive to salinity; seven species were tolerance. The results were concluded that the properties of water were tolerable for the fish community. Ultimately, it can be concluded that the sector of MOD supported the fish community. But it needs constant monitoring for the conserve and development of the diversity and the case of the healthy fish.
Effect of Phenylalanine, Jasmonic Acid and Biofertilizer on Growth, Yield and Anthocyanin Pigments of Roselle Calyces
Authors: Al-Mohammad Maher H.S. , Sachet Thamena F.K. , Al-dulaimi Zaman Salah 
Published: November 2021

Abstract
Experiment was conducted during summer season of 2021 to study the effect of foliar applications of Phenylalanine (Phe), Jasmonic acid (JA), Biofertilizer (Bio) and their combinations (Phe+JA, Phe+Bio, JA+Bio, Phe+JA+Bio and control) on some growth, yield parameters and anthocyanin compounds on Roselle calyces Hibiscus sabdariffa L., The treatments were distributed on Randomized Completely Block Design in three replicates, means were compared according to the Least Significant Difference test (LSD) at probability level of 0.05. The results show that growth parameters (plant height, branches number, leave area and chlorophyll) and yield parameters (fruits number, fresh weight calyces, fresh yield calyces and dry yield calyces) were increased due by foliar application Phe+JA+Bio, while the combination treatment Phe+Bio gave significant effects on vitamin C, total anthocyanin, Gossypetin, Sabdartine, Hibescitine, Delphidine and Cyanidin of calyces reached at 201.5, 184.5, 29.3, 31.2, 57.4, 35.4, 27.8 (mg.100g) respectively.
Characterization and Evaluation The Biological Activity of Prepared Nano-Gentamicin Nanoparticles
Authors: Kamal Rana A. , Kahdhum Quraish A. , Mohammed Awatif I. , Essa Ahmed J. , Abd elhamid Maan A. , Mohamad Essraa A. , Abod Salma A. , Jasim Gharam H. 
Published: November 2021

Abstract
Nanotechnology is a capable approach to enhance the activity of common antimicrobial agent mainly used in human and veterinary drug. Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature (80)0C. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM), atomic force microscopy (AFM) and antibacterial effect of both Nano and stander gentamycin were analyzed. The results showed that the average size of gentamycin nanoparticles was 68.51 nanometers with homogeneous distribution. The EDX showed large number of elements present in the Nano solution. The antibacterial effect of Nano gentamycin took a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, where the inhibition diameter for E.coli was 29 mm, and for St. epidermidis was 27 mm. The MIC of gentamicin nanostructures was the concentration 0.00125 mg/ml.
Nonlinear parabolic equations with Robin boundary conditions and Hardy-Leray type inequalities
Authors: Goldstein Gisèle , Goldstein Jerome , Kömbe Ismail , Tellioğlu Balekoğlu Reyhan 
Published: November 2021

We are primarily concerned with the absence of positive solutions of the following problem,
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OPUS-Rota4: a gradient-based protein side-chain modeling framework assisted by deep learning-based predictors
Authors: Xu Gang , Wang Qinghua , Ma Jianpeng 
Published: November 2021

Abstract
Accurate protein side-chain modeling is crucial for protein folding and protein design. In the past decades, many successful methods have been proposed to address this issue. However, most of them depend on the discrete samples from the rotamer library, which may have limitations on their accuracies and usages. In this study, we report an open-source toolkit for protein side-chain modeling, named OPUS-Rota4. It consists of three modules: OPUS-RotaNN2, which predicts protein side-chain dihedral angles; OPUS-RotaCM, which measures the distance and orientation information between the side chain of different residue pairs and OPUS-Fold2, which applies the constraints derived from the first two modules to guide side-chain modeling. OPUS-Rota4 adopts the dihedral angles predicted by OPUS-RotaNN2 as its initial states, and uses OPUS-Fold2 to refine the side-chain conformation with the side-chain contact map constraints derived from OPUS-RotaCM. Therefore, we convert the side-chain modeling problem into a side-chain contact map prediction problem. OPUS-Fold2 is written in Python and TensorFlow2.4, which is user-friendly to include other differentiable energy terms. OPUS-Rota4 also provides a platform in which the side-chain conformation can be dynamically adjusted under the influence of other processes. We apply OPUS-Rota4 on 15 FM predictions submitted by AlphaFold2 on CASP14, the results show that the side chains modeled by OPUS-Rota4 are closer to their native counterparts than those predicted by AlphaFold2 (e.g. the residue-wise RMSD for all residues and core residues are 0.588 and 0.472 for AlphaFold2, and 0.535 and 0.407 for OPUS-Rota4).
Profitability of small- and medium-sized enterprises in Marshall’s time: sector and spatial heterogeneity in the nineteenth century
Authors: Bennett Robert J , Smith Harry , Montebruno Piero , van Lieshout Carry 
Published: November 2021

AbstractNew data on profit heterogeneity of small- and medium-sized firms for 1861–81 in England and Wales are used to reinterpret Marshall's contemporary insights. Profit level differences are chiefly explained by location, mainly urbanisation effects. But profitability (profit per worker) is mainly explained by sectors, at both 1-digit and 5-digit level. Sector market opportunities reflected barriers to market entry which limited substitutability for the services of the professions, some manufacturing and maker-dealing industries. Localisation mainly reflected urban/rural differences, accessibility to railways and to a lesser extent waterways. Differences in firm-level organisation (measured by portfolio diversification and partnerships) were less significant for explaining profit heterogeneity than sector or localisation. Demographic effects such as an entrepreneur's age had little significance. Marshall's insight of convergence to mean industry-sector profitability, with localisation as a secondary influence, is confirmed, but there remain unexplained elements of heterogeneity indicating important roles of entrepreneurial agency.
Non-lipid-rich low attenuation plaque with intraplaque haemorrhage assessed by multimodality imaging: a case report
Authors: Matsumoto Hidenari , Xie Yibin , Li Debiao , Shinke Toshiro 
Published: November 2021

Abstract

Background
The lipid-rich necrotic core is a major pathological hallmark of acute coronary syndrome. Low attenuation plaque (LAP) on coronary computed tomography angiography (CCTA), defined as plaque CT attenuation of &lt;30 Hounsfield units, is commonly believed to correspond to the lipid component. This report presents a non-lipid-rich LAP with intraplaque haemorrhage of the left main coronary artery (LM), as assessed by CCTA, near-infrared spectroscopy (NIRS), and non-contrast magnetic resonance imaging (MRI) using coronary atherosclerosis T1-weighted characterization with integrated anatomical reference technique, recently developed by our group.

Case summary
A 75-year-old woman presented with chest discomfort on exertion. Coronary computed tomography angiography revealed severe stenosis of the mid-left circumflex coronary artery and minimal stenosis with a large eccentric LM plaque. The LM lesion had an LAP, with a minimum plaque attenuation of 25 Hounsfield units. On non-contrast T1-weighted MRI, a high-intensity plaque with a plaque-to-myocardium signal intensity ratio of 3.02 was observed within the vessel wall, indicating intraplaque haemorrhage. Near-infrared spectroscopy categorized the lesion as non-lipid-rich, with a maximum lipid core burden index in 4 mm of 169.

Discussion
Intraplaque haemorrhage is a key feature of plaque instability, which is different from the lipid-rich necrotic core. Non-contrast T1-weighted MRI is ideal for detecting intraplaque haemorrhage with short T1 values. The imaging findings suggest that LAP on CCTA may represent not only lipid-rich plaques but also intraplaque haemorrhage. Magnetic resonance imaging provides a unique insight into plaque vulnerability from a different perspective than lipid assessment. Multimodality imaging, including MRI, facilitates the understanding of complicated plaque morphologies.

Keywords
Atherosclerosis • Case report • Computed tomography • Intraplaque haemorrhage • Lipid-rich plaque • Magnetic resonance imaging • Near-infrared spectroscopy-intravascular ultrasound
The association of PRECISE-DAPT score with ischaemic outcomes in patients taking dual antiplatelet therapy following percutaneous coronary intervention: a meta-analysis
Authors: Clifford Cole R , Boudreau Rene , Visintini Sarah , Orr Nathan , Fu Angel Y N , Malhotra Nikita , Barry Quinton , So Derek Y F 
Published: November 2021

Abstract

Aims
The PRECISE-DAPT (Predicting Bleeding Complication in Patients Undergoing Stent Implantation and Subsequent Dual Antiplatelet Therapy) score identifies patients at high risk of bleeding complications following percutaneous coronary intervention (PCI). International guidelines recommend the PRECISE-DAPT score to identify patients at high risk for bleeding, who may benefit from shortened dual antiplatelet therapy. The association of the PRECISE-DAPT score with ischaemic outcomes remains unclear. We performed a meta-analysis investigating the association between a high PRECISE-DAPT score and ischaemic outcomes.

Methods and results
A comprehensive literature search was conducted on articles published between 11 March 2017 and 5 June 2021. Two reviewers independently screened articles for inclusion using pre-defined criteria. The outcome measures extracted included composite ischaemic events, major bleeding events, and all-cause mortality. A random effects model was applied to obtain combined risk estimates for outcomes. From 12 included studies, there were 39 459 patients with PRECISE-DAPT &lt;25 and 14 761 patients with PRECISE-DAPT ≥25. PRECISE-DAPT score ≥25 was associated with increased risk of composite ischaemic events [odds ratio (OR) 2.16; 95% confidence interval (CI) 1.77–2.65], myocardial infarction (OR 2.06; 95% CI 1.38–3.08), and ischaemic stroke (OR 2.90; 95% CI 1.76–4.78). Patients with a PRECISE-DAPT score ≥25 had increased risk of major bleeding (OR 3.62; 95% CI 2.62–4.99). Patients with a PRECISE-DAPT score ≥25 had higher risk of all-cause mortality (OR 5.83; 95% CI 5.37–6.33).

Conclusion
Patients with a PRECISE-DAPT score ≥25 are at increased risk for ischaemic events, bleeding, and all-cause mortality. Prospective evaluation of a PRECISE-DAPT guided approach to antiplatelet therapy is required to demonstrate benefit in this high-risk population.
Chronic kidney disease and subclinical abnormalities of left heart mechanics in the community
Authors: Iwama Kentaro , Nakanishi Koki , Daimon Masao , Yoshida Yuriko , Sawada Naoko , Hirose Kazutoshi , Yamamoto Yuko , Ishiwata Jumpei , Hirokawa Megumi , Kaneko Hidehiro , Nakao Tomoko , Mizuno Yoshiko , Morita Hiroyuki , Di Tullio Marco R , Homma Shunichi , Komuro Issei 
Published: November 2021

AbstractAimsCardiovascular disease is the leading cause of death in chronic kidney disease (CKD) patients, although the pathophysiological mechanisms are not fully studied. This study aimed to determine whether CKD could adversely affect subclinical left heart function in a sample of the general population without cardiac disease.Methods and resultsWe examined 1158 participants who voluntarily underwent extensive cardiovascular examination including laboratory test and two-dimensional speckle-tracking echocardiography to assess left ventricular global longitudinal strain (LVGLS) and left atrial (LA) reservoir, conduit, and pump strain. According to the estimated glomerular filtration rate (eGFR), participants were classified into four groups; Stage 1 (n = 112; eGFR ≥90 mL/min/1.73 m2), Stage 2 (n = 818; 60–89 mL/min/1.73 m2), Stage 3a (n = 191; 45–59 mL/min/1.73 m2), and Stage 3b–5 (n = 37; eGFR &lt;45 mL/min/1.73 m2). Progressive declines of LVGLS, LA reservoir, and conduit strain were observed according to the severity of CKD (P &lt; 0.001), while LA pump strain did not differ between the groups. In multivariable analyses, eGFR was associated with LVGLS (standardized β = −0.068, P = 0.019) as well as LA reservoir (standardized β = 0.117, P &lt; 0.001) and conduit strain (standardized β = 0.130, P &lt; 0.001), independent of traditional cardiovascular risk factors, pertinent biomarkers, and LV geometry and diastolic function. The independent association between eGFR and LA strain persisted even after adjustment for LVGLS.ConclusionWorsening renal function was independently associated with impaired LV/LA strain in an unselected community-based cohort. The assessment of LV and LA strain may allow better risk stratification in CKD patients.
Pre-Colonial Warfare and Long-Run Development in India
Authors: Dincecco Mark , Fenske James , Menon Anil , Mukherjee Shivaji 
Published: November 2021

Abstract
Does pre-colonial history—and in particular the role of interstate warfare—help explain long-run development patterns across India? To address this question, we construct a new geocoded database of historical conflicts on the Indian subcontinent. We document a robust positive relationship between pre-colonial conflict exposure and local economic development today. Drawing on archival and secondary data, we show that districts that were more exposed to pre-colonial conflict experienced greater early state-making, followed by lower political violence and higher investments in physical and human capital in the long term.
Teacher strategies in implementing English medium instruction
Authors: Richards Jack C , Pun Jack 
Published: November 2021

AbstractWith the expanding role of English medium instruction (EMI) worldwide there has been an increasing use of English to teach content subjects at tertiary level. In implementing EMI a common assumption has been that the primary issue affecting successful EMI is the English proficiency level of the EMI instructor. This paper reviews this assumption by exploring three dimensions of EMI—the cognitive, the genre, and the academic task dimensions of teaching in a discipline—and the role that English plays in developing disciplinary literacy and competence. From a review of EMI teachers’ accounts of EMI in Hong Kong as well as other EMI contexts, information is presented to demonstrate the ways in which EMI teachers adapt their teaching in order to prepare for and manage EMI. Goals are suggested for the professional development of EMI teachers.
The cold chain and the COVID-19 pandemic: an unusual increase in histamine content in fish samples collected in Southern Italy during lockdown
Authors: Galluzzo Francesco Giuseppe , Cammilleri Gaetano , Cicero Antonello , Pantano Licia , Pulvirenti Andrea , Macaluso Andrea , Cicero Nicola , Calabrese Vittorio , Ferrantelli Vincenzo 
Published: November 2021

AbstractObjectivesWe analysed 900 samples of fresh (250) and processed (650) fish products collected in Sicily (Southern Italy) in 2020 during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic (hereafter: COVID-19).Materials and methodsThe samples were divided temporally based on four phases relating to the various restrictions imposed by the Italian government in this period. The validated method of ultrahigh-performance liquid chromatography combined with a diode array detector was then employed for the analysis.ResultsThe samples collected during the Phase I lockdown period and after it had ended (Phase II) revealed significant increases in the mean histamine levels: (41.89±87.58) mg/kg and (24.91±76.76) mg/kg, respectively. The 11 (1.3% of the total) fresh fish samples that were identified as being non-compliant with Regulation (EC) No.2073/2005 were only found during these two periods. All the processed samples were always compliant. The histamine values decreased as the restrictions eased, achieving a mean value of (11.16±9.3) mg/kg (Phase III).ConclusionsThere was an increase in the incidence of fish samples that were non-compliant with Regulation (EC) No.2073/2005 compared to previous surveillance data. These results provide a first report on the effect of lockdown measures on food safety and the cold chain. Our findings must cause food safety operators to intensify their controls over fresh fish products in such periods to safeguard consumer health. Further studies are required to evaluate whether the same trend would be observed with other food contaminants.
Cecropins contribute toDrosophilahost defense against a subset of fungal and Gram-negative bacterial infection
Authors: Carboni Alexia L , Hanson Mark A , Lindsay Scott A , Wasserman Steven A , Lemaitre Bruno 
Published: November 2021

AbstractCecropins are small helical secreted peptides with antimicrobial activity that are widely distributed among insects. Genes encoding Cecropins are strongly induced upon infection, pointing to their role in host defense. In Drosophila, four cecropin genes clustered in the genome (CecA1, CecA2, CecB, and CecC) are expressed upon infection downstream of the Toll and Imd pathways. In this study, we generated a short deletion ΔCecA-C removing the whole cecropin locus. Using the ΔCecA-C deficiency alone or in combination with other antimicrobial peptide (AMP) mutations, we addressed the function of Cecropins in the systemic immune response. ΔCecA-C flies were viable and resisted challenge with various microbes as wild-type. However, removing ΔCecA-C in flies already lacking 10 other AMP genes revealed a role for Cecropins in defense against Gram-negative bacteria and fungi. Measurements of pathogen loads confirm that Cecropins contribute to the control of certain Gram-negative bacteria, notably Enterobacter cloacae and Providencia heimbachae. Collectively, our work provides the first genetic demonstration of a role for Cecropins in insect host defense and confirms their in vivo activity primarily against Gram-negative bacteria and fungi. Generation of a fly line (ΔAMP14) that lacks 14 immune inducible AMPs provides a powerful tool to address the function of these immune effectors in host–pathogen interactions and beyond.
Genome-wide association mapping of transcriptome variation inMimulus guttatusindicates differing patterns of selection oncis- versustrans-acting mutations
Authors: Brown Keely E , Kelly John K 
Published: November 2021

AbstractWe measured the floral bud transcriptome of 151 fully sequenced lines of Mimulus guttatus from one natural population. Thousands of single nucleotide polymorphisms (SNPs) are implicated as transcription regulators, but there is a striking difference in the allele frequency spectrum of cis-acting and trans-acting mutations. Cis-SNPs have intermediate frequencies (consistent with balancing selection) while trans-SNPs exhibit a rare-alleles model (consistent with purifying selection). This pattern only becomes clear when transcript variation is normalized on a gene-to-gene basis. If a global normalization is applied, as is typically in RNAseq experiments, asymmetric transcript distributions combined with “rarity disequilibrium” produce a superabundance of false positives for trans-acting SNPs. To explore the cause of purifying selection on trans-acting mutations, we identified gene expression modules as sets of coexpressed genes. The extent to which trans-acting mutations influence modules is a strong predictor of allele frequency. Mutations altering expression of genes with high “connectedness” (those that are highly predictive of the representative module expression value) have the lowest allele frequency. The expression modules can also predict whole-plant traits such as flower size. We find that a substantial portion of the genetic (co)variance among traits can be described as an emergent property of genetic effects on expression modules.