bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors
Authors: Tsitrina Alexandra A , Krasylov Igor V , Maltsev Dmitry I , Andreichenko Irina N , Moskvina Viktoria S , Ivankov Dmitry N , Bulgakova Elena V , Nesterchuk Mikhail , Shashkovskaya Vera , Dashenkova Nataliya O , Khilya Vladimir P , Mikaelyan Arsen , Kotelevtsev Yuri 
Published: April 2021

Abstract
Elevated plasma levels of hyaluronic acid (HA) is a disease marker in liver pathology and other inflammatory disorders. Inhibition of HA synthesis with coumarin 4-methylumbelliferone (4MU) has a beneficial effect in animal models of fibrosis, inflammation, cancer and metabolic syndrome. 4MU is an active compound of approved choleretic drug hymecromone with low bioavailability and a broad spectrum of action. New, more specific and efficient inhibitors of hyaluronan synthases (HAS) are required. We have tested several newly synthesized coumarin compounds and commercial chitin synthesis inhibitors to inhibit HA production in cell culture assay. Coumarin derivative compound VII (10′-methyl-6′-phenyl-3′H-spiro[piperidine-4,2′-pyrano[3,2-g]chromene]-4′,8′-dione) demonstrated inhibition of HA secretion by NIH3T3 cells with the half-maximal inhibitory concentration (IC50) = 1.69 ± 0.75 μΜ superior to 4MU (IC50 = 8.68 ± 1.6 μΜ). Inhibitors of chitin synthesis, etoxazole, buprofezin, triflumuron, reduced HA deposition with IC50 of 4.21 ± 3.82 μΜ, 1.24 ± 0.87 μΜ and 1.48 ± 1.44 μΜ, respectively. Etoxazole reduced HA production and prevented collagen fibre formation in the CCl4 liver fibrosis model in mice similar to 4MU. Bioinformatics analysis revealed homology between chitin synthases and HAS enzymes, particularly in the pore-forming domain, containing the proposed site for etoxazole binding.
The Integrative Biology of Pigment Organelles, a Quantum Chemical Approach
Authors: Figon Florent , Casas Jérôme 
Published: April 2021

Synopsis
Coloration is a complex phenotypic trait involving both physical and chemical processes at a multiscale level, from molecules to tissues. Pigments, whose main property is to absorb specific wavelengths of visible light, are usually deposited in specialized organelles or complex matrices comprising proteins, metals, ions, and redox compounds, among others. By modulating electronic properties and stability, interactions between pigments and these molecular actors can lead to color tuning. Furthermore, pigments are not only important for visual effects but also provide other critical functions, such as detoxification and antiradical activity. Hence, integrative studies of pigment organelles are required to understand how pigments interact with their cellular environment. In this review, we show how quantum chemistry, a computational method that models the molecular and optical properties of pigments, has provided key insights into the mechanisms by which pigment properties, from color to reactivity, are modulated by their organellar environment. These results allow us to rationalize and predict the way pigments behave in supramolecular complexes, up to the complete modeling of pigment organelles. We also discuss the main limitations of quantum chemistry, emphasizing the need for carrying experimental work with identical vigor. We finally suggest that taking into account the ecology of pigments (i.e., how they interact with these various other cellular components and at higher organizational levels) will lead to a greater understanding of how and why animals are vividly and variably colored, two fundamental questions in organismal biology.
Oral and Palmitate Paliperidone Long-Acting Injectable Formulations’ Use in Schizophrenia Spectrum Disorders: A Retrospective Cohort Study from the First Episode Psychosis Intervention Program (CRUPEP)
Authors: Segarra R , Recio-Barbero M , Sáenz-Herrero M , Mentxaka O , Cabezas-Garduño J , Eguíluz J I , Callado L F 
Published: April 2021

Abstract

Background
Long-acting injectable antipsychotics (LAIs) may be a suitable therapeutic option for those patients in earlier stages of psychosis to avoid relapses and disease progression. Despite that, there is a lack of evidence in the literature regarding the use of LAIs in this profile of patients.

Methods
This is a retrospective cohort analysis to assess the efficacy, tolerability, and pattern of use of palmitate paliperidone long-acting injectable (PPLAI) formulations (1- and 3-month doses) compared to oral paliperidone/risperidone in patients with a nonaffective first episode of psychosis (FEP) over 12 months of follow-up. Relevant sociodemographic and clinical information were assessed, as well as main clinical scales: Positive and Negative Syndrome Scale, Personal and Social Performance Scale, and Clinical Global Impression Scale Improvement and Severity measures.

Results
The study included 48 patients, 16 per arm, who were aged 20–50 years and had an FEP. Significant improvements were registered for all treatment groups. Despite that, patients receiving PPLAI 1- and 3-month formulations obtained greater improvements than those in the oral group in the main domains assessed (P &lt; .001). We found no statistically significant differences in hospitalizations between groups. Side effects were presented in 24% of patients. A trend towards reducing antipsychotic doses was observed in 43.8% of patients to achieve the minimum effective dose and avoid the occurrence of side effects.

Conclusions
To our knowledge, this is the first study assessing the use of palmitate paliperidone long-acting formulations versus oral risperidone or paliperidone in FEP. Treatment with PPLAI formulations seems to be an effective therapeutic choice at earlier stages of the disease.
Safety, Tolerability, and Pharmacokinetics of a Long-Acting Broadly Neutralizing Human Immunodeficiency Virus Type 1 (HIV-1) Monoclonal Antibody VRC01LS in HIV-1–Exposed Newborn Infants
Authors: McFarland Elizabeth J , Cunningham Coleen K , Muresan Petronella , Capparelli Edmund V , Perlowski Charlotte , Morgan Patricia , Smith Betsy , Hazra Rohan , Purdue Lynette , Harding Paul A , Theron Gerhard , Mujuru Hilda , Agwu Allison , Purswani Murli , Rathore Mobeen H , Flach Britta , Taylor Alison , Lin Bob C , McDermott Adrian B , Mascola John R , Graham Barney S ,   International Maternal Pediatric Adolescent AIDS Clinical Trials Network (IMPAACT) P1112 Team, Rossouw Magdel , Rossouw Lindie , Louw Jeanne , Vhembo Tichaona , Mhembere Tsungai Patience , Matibe Petronella , Mahmoudi Saniyyah , Maldonado Alexandrea , Maraqa Nizar , Baig Mahboobullah M , Rogo Tanya , Cavallo Martha , Collinson-Streng Aleisha , Anderson Thuy , Golden W Christopher , Persaud Deborah , Puga Ana M , Robinson Lisa-Gaye , Eysallenne Zulma , Leon Dayana , Paul Mary E , McMullen-Jackson Chivon , Buschur Shelley , Pontifes Mariam , Sung Joyce , Glenny Carrie , Dunn Jennifer , Navarro Kacey 
Published: April 2021

Abstract

Background
Perinatal human immunodeficiency virus type 1 (HIV-1) continues to occur due to barriers to effective antiretroviral prevention that might be mitigated by long-acting broadly neutralizing monoclonal antibodies (bNAbs).

Methods
An extended half-life bNAb, VRC01LS, was administered subcutaneously at 80 mg/dose after birth to HIV-1–exposed, nonbreastfed (cohort 1, n = 10) and breastfed (cohort 2, n = 11) infants. Cohort 2 received a second dose (100 mg) at 12 weeks. All received antiretroviral prophylaxis. VRC01LS levels were compared to VRC01 levels determined in a prior cohort.

Results
Local reactions (all grade ≤2) occurred in 67% and 20% after dose 1 and dose 2, respectively. The weight-banded dose (mean 28.8 mg/kg) of VRC01LS administered subcutaneously achieved a mean (standard deviation) plasma level of 222.3 (71.6) µg/mL by 24 hours and 44.0 (11.6) µg/mL at week 12, prior to dose 2. The preestablished target of ≥50 µg/mL was attained in 95% and 32% at weeks 8 and 12, respectively. The terminal half-life was 37–41 days. VRC01LS level after 1 dose was significantly greater (P &lt;.002) than after a VRC01 dose (20 mg/kg). No infants acquired HIV-1.

Conclusions
VRC01LS was well tolerated with pharmacokinetics that support further studies of more potent long-acting bNAbs as adjunct treatment with antiretrovirals to prevent infant HIV-1 transmission.
CD49d marks Th1 and Tfh-like antigen-specific CD4+ T cells during Plasmodium chabaudi infection
Authors: Jian Jiun-Yu , Inoue Shin-Ichi , Bayarsaikhan Ganchimeg , Miyakoda Mana , Kimura Daisuke , Kimura Kazumi , Nozaki Eriko , Sakurai Takuya , Fernandez-Ruiz Daniel , Heath William R , Yui Katsuyuki 
Published: April 2021

Abstract
Upon activation, specific CD4+ T cells up-regulate the expression of CD11a and CD49d, surrogate markers of pathogen-specific CD4+ T cells. However, using T-cell receptor transgenic mice specific for a Plasmodium antigen, termed PbT-II, we found that activated CD4+ T cells develop not only to CD11ahiCD49dhi cells, but also to CD11ahiCD49dlo cells during acute Plasmodium infection. CD49dhi PbT-II cells, localized in the red pulp of spleens, expressed transcription factor T-bet and produced IFN-γ, indicating that they were type 1 helper T (Th1)-type cells. In contrast, CD49dlo PbT-II cells resided in the white pulp/marginal zones and were a heterogeneous population, with approximately half of them expressing CXCR5 and a third expressing Bcl-6, a master regulator of follicular helper T (Tfh) cells. In adoptive transfer experiments, both CD49dhi and CD49dlo PbT-II cells differentiated into CD49dhi Th1-type cells after stimulation with antigen-pulsed dendritic cells, while CD49dhi and CD49dlo phenotypes were generally maintained in mice infected with Plasmodium chabaudi. These results suggest that CD49d is expressed on Th1-type Plasmodium-specific CD4+ T cells, which are localized in the red pulp of the spleen, and can be used as a marker of antigen-specific Th1 CD4+ T cells, rather than that of all pathogen-specific CD4+ T cells.
Initial Experience Combining Negative Pressure Wound Therapy With Autologous Skin Cell Suspension and Meshed Autografts
Authors: Carney Bonnie C , Johnson Laura S , Shupp Jeffrey W , Travis Taryn E 
Published: April 2021

Abstract
The success of autologous split-thickness skin grafts (STSGs) in the treatment of full-thickness burns is often dependent on the dressing used to secure it. Tie-over bolsters have been used traditionally; however, they can be uncomfortable for patients and preclude grafting large areas in one definitive operation. Negative pressure wound therapy (NPWT) is used as an alternative to bolster dressings and may afford additional wound healing benefits. In our center, NPWT has become the dressing of choice for securing STSGs. While the RECELL® system is being used in conjunction with STSGs, it is currently unknown whether autologous skin cell suspensions (ASCS) can be used with NPWT. This report is a retrospective chart review of nine patients treated in this manner. All wounds were almost completely re-epithelialized within 14 days, and their healing was as expected. Wound healing trajectories are shown. There were no significant complications in these patients. This dressing technique can be considered as an option when using ASCS and widely meshed STSG.
Efficacy and Nontarget Effects of Net Exclusion Enclosures on Apple Pest Management
Authors: Marshall A T , Beers E H 
Published: April 2021

Abstract
Management of direct apple pests, such as codling moth, continues to be problematic despite the widespread implementation of behavioral and chemical controls. Apple growers have increased their use of netting to protect fruit from environmental injury, with some structures enclosing the entire orchard. These enclosures represent a new pest management tactic through physical exclusion. We conducted a two-year trial to examine the effects of full exclusion netting on pests and natural enemies of apples. Insect densities and damage in trees under fully enclosed (net) cages were compared with conventionally (insecticide only) treated and untreated plots. Caged plots had 18.1- and 11.4-fold less codling moth damage than the check, and 4.9- and 4.2-fold less damage than the insecticide-only plots in 2016 and 2017, respectively. However, densities of woolly apple aphid and its parasitoid Aphelinus mali (Haldeman) (Hymenoptera: Aphelinidae) were significantly greater in the caged plots. Densities of earwigs, a typically flightless generalist predator, were not different among treatments, while adults of more mobile flying generalist predators, lacewings and syrphids, were significantly lower in cages compared with uncaged plots. These results demonstrate that although biological control may be partially disrupted, net enclosures have significant potential as a holistic apple management technique.
Quantifying the Contribution of Seed Blended Refugia in Field Corn to Helicoverpa zea (Lepidoptera: Noctuidae) Populations
Authors: Towles T B , Buntin G D , Catchot A L , Gore J , Cook D R , Caprio M A , Daves C 
Published: April 2021

Abstract
Helicoverpa zea (Boddie), a pest of cotton that also occurs in field corn, is commonly controlled through the use of foliar-applied insecticides or transgenic crops expressing Bacillus thuringiensis (Berliner) (Bt) genes. To minimize the risk of Bt resistance in pest populations, refuge systems have been implemented for sustainable agroecosystem management. Historically, structured refuge compliance among growers has been low, leading to the commercialization of seed blended refugia. To test the viability of seed blended refugia in southern U.S. field corn, field studies were conducted in Mississippi and Georgia during 2016, 2017, and 2018 growing seasons. To quantify adult H. zea emergence from structured (non-Bt corn) and seed blended refuge options, emergence traps were utilized. Kernel damage among seed blended refuge and structured refuge corn ears were recorded and compared. The timing of moth emergence was recorded. When compared to a structured refuge, H. zea adult moth emergence from seed blended refugia did not significantly differ. Kernel damage of non-Bt plants in the seed blended treatments was not significantly different than non-Bt plants in the structured refuge treatments. Moth emergence timing was not significantly delayed between the structured refuge and seed blended refuge treatments. Results of this study suggest that a seed blended refuge may provide an effective insecticide resistance management alternative for H. zea in areas where structured refuge compliance is low.
Impacts of Fire on Butterfly Genetic Diversity and Connectivity
Authors: Gates Daisy , Jackson Breeanne , Schoville Sean D 
Published: April 2021

AbstractHow do novel fire regimes and a long history of fire suppression influence species genetic diversity? Genetic diversity provides the raw materials for sustaining viable populations and for allowing adaptation to novel environmental challenges, and at present, few studies address the genetic responses of animals to fire management. Here we study the genetic responses of 2 butterfly species to a landscape gradient of fire timing and severity in Yosemite National Park using a large set of genome-wide single nucleotide polymorphisms (SNPs). Butterflies are important bio-indicators of invertebrate diversity and play important roles in both bottom–up and top–down ecosystem processes, and typically increase in abundance following wildfires, due to an increase in abundance of flowering plants. However, it is not clear how genetic diversity and genetic connectivity of butterflies respond to landscape change following fire, and whether fire management has positive or negative effects. We found evidence to suggest that fire increases genetic diversity and reduces isolation in 2 butterfly species, but that aspects of the fire regime (severity, extent, timing, and frequency) differ in importance depending on the ecology of the specific species. This research is the first study to address fire management impacts on genetic diversity in invertebrates, and the results will allow fire managers to predict that fire reintroduction in protected areas will generally benefit butterfly populations.
Transcriptome Analysis of Myzus persicae to UV-B Stress
Authors: Yang Chang-Li , Meng Jian-Yu , Yao Meng-Shuang , Zhang Chang-Yu 
Published: April 2021

Abstract
As an environmental stress factor, ultraviolet-B (UV-B) radiation directly affects the growth and development of Myzus persicae (Sulzer) (Homoptera: Aphididae). How M. persicae responds to UV-B stress and the molecular mechanisms underlying this adaptation remain unknown. Here, we analyzed transcriptome data for M. persicae following exposure to UV-B radiation for 30 min. We identified 758 significant differentially expressed genes (DEGs) following exposure to UV-B stress, including 423 upregulated and 335 downregulated genes. In addition, enrichment analysis using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases illustrated that these DEGs are associated with antioxidation and detoxification, metabolic and protein turnover, immune response, and stress signal transduction. Simultaneously, these DEGs are closely related to the adaptability to UV-B stress. Our research can raise awareness of the mechanisms of insect responses to UV-B stress.
The inhibitory effect of trimetazidine on detrusor contractility – a potential repositioning of trimetazidine for the treatment of overactive bladder
Authors: Engin Seckin , Kaya Yasar Yesim , Barut Elif Nur , Getboga Damla , Erac Yasemin , Sezen Sena F 
Published: April 2021

Abstract

Objectives
This study aimed to identify the effect of trimetazidine (TMZ), an antianginal drug, on detrusor smooth muscle (DSM) contractility and its possible mechanisms of action.

Methods
We performed in-vitro contractility studies on isolated mouse DSM strips and investigated the effect of TMZ on Ca2+ levels in fura-2-loaded A7r5 cells.

Key ﬁndings
TMZ (300 or 1000 µM) inhibited carbachol (CCh)- and KCl-induced contractions and produced a concentration-dependent (10–1000 µM) relaxation in KCl-precontracted DSM strips. TMZ-induced relaxation was markedly decreased by BaCl2, an inward-rectifying K+ channel blocker, but was not altered by preincubation with tetraethylammonium, glibenclamide, 4-aminopyridine, propranolol, L-NAME or methylene blue. TMZ (300 or 1000 µM) reduced both the CaCl2-induced contraction of depolarized DSM strips under Ca2+-free conditions and the CCh-induced contraction of DSM strips preincubated with nifedipine in Ca2+-containing Krebs solution. Furthermore, TMZ (1000 µM) significantly decreased the Ca2+ levels in fura-2-loaded A7r5 cells.

Conclusions
TMZ decreased DSM contractility and caused a concentration-dependent relaxation of the tissue possibly through its actions on Ca2+ transients and K+ channels. Our results provide preclinical evidence that TMZ would be a potential candidate to treat disorders related to the overactivity of the bladder.
Pathologic validation of an yttrium-90 trans-arterial radioembolization dosimetry: a case report
Authors: Baikovitz Jacqueline B , Thornton Lindsay , Garcia-Buitrago Monica T , Livingstone Alan S , Studenski Matthew T , Portelance Lorraine 
Published: April 2021

Abstract
Yttrium-90 (Y-90) trans-arterial radioembolization (TARE) is used in the management of unresectable hepatocellular carcinoma (HCC). During the last 5 years, dosimetry software has been developed to allow for a more rigorous approach of dose prescription in Y-90 TARE. We present here a case study of a 77-year-old woman diagnosed with HCC, who underwent a Y-90 TARE as a bridge procedure to liver resection. This clinical scenario represents a unique opportunity to illustrate the predictive value of dosimetric findings correlating dosimetry with pathological findings. In this case, Y-90 TARE dosimetry was predictive of treatment response in which the tumor received a mean dose of 156 Gy and demonstrated a complete pathologic response.
Minimally invasive approach in radio distal end fractures, three different types of incisions
Authors: Romero Prieto Fernando Xavier , Reyes García Carlos Raúl 
Published: April 2021

Abstract
Two techniques are described to treat distal radius fractures: conventional (Henry approach) and minimally invasive plate osteosynthesis. The latter technique has been described by different authors such as Imatani et al. and Zenke et al.
This was a descriptive retrospective study, analyzing 26 adult patients with unstable distal radius fracture, extra-articular type A or partially intra-articular type B according to AO. The approaches used were: (i) single longitudinal palmar incision; (ii) double T-incision (horizontal and vertical) and (iii) double position II.
Ages were between 21 and 78 years. Most affected hand was the right. Most common fracture was 23B2 (AO classification). In total, 84.6% of the patients did not present complications. According to the functional evaluation of the wrist by the Mayo Clinic, 31% showed excellent results, 42% showed good results, 27% showed fair results.
The techniques had satisfactory results for the osteosynthesis with more aesthetic and less invasive approach.
Phenotypic diploidization in plant functional traits uncovered by synthetic neopolyploids inDianthus broteri
Authors: Domínguez-Delgado Juan José , López-Jurado Javier , Mateos-Naranjo Enrique , Balao Francisco 
Published: April 2021

AbstractWhole-genome duplication and post-polyploidization genome downsizing play key roles in the evolution of land plants; however, the impact of genomic diploidization on functional traits still remains poorly understood. Using Dianthus broteri as a model, we compared the ecophysiological behaviour of colchicine-induced neotetraploids (4xNeo) to diploids (2x) and naturally occurring tetraploids (4xNat). Leaf gas-exchange and chlorophyll fluorescence analyses were performed in order to asses to what extent post-polyploidization evolutionary processes have affected 4xNat. Genomic diploidization and phenotypic novelty were evident. Distinct patterns of variation revealed that post-polyploidization processes altered the phenotypic shifts directly mediated by genome doubling. The photosynthetic phenotype was affected in several ways but the main effect was phenotypic diploidization (i.e. 2x and 4xNat were closer to each other than to 4xNeo). Overall, our results show the potential benefits of considering experimentally synthetized versus naturally established polyploids when exploring the role of polyploidization in promoting functional divergence.
Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development
Authors: Ko Swee-Suak , Li Min-Jeng , Ho Yi-Cheng , Yu Chun-Ping , Yang Ting-Ting , Lin Yi-Jyun , Hsing Hung-Chien , Chen Tien-Kuan , Jhong Chung-Min , Li Wen-Hsiung , Sun-Ben Ku Maurice 
Published: April 2021

Abstract
GIBBERELLIN MYB GENE (GAMYB), UNDEVELOPED TAPETUM1 (UDT1), TDR INTERACTING PROTEIN2 (TIP2/bHLH142), TAPETUM DEGENERATION RETARDATION (TDR), and ETERNAL TAPETUM 1/DELAYED TAPETUM DEGENERATION (EAT1/DTD) are important transcription factors that play a crucial role during pollen development in rice. This study demonstrates that bHLH142 acts downstream of UDT1 and GAMYB and works as a ‘hub’ in these two pollen pathways. We show that GAMYB modulates bHLH142 expression through specific binding to the MYB motif of the bHLH142 promoter during the early stage of pollen development, while TDR acts as a transcriptional repressor of the GAMYB modulation of bHLH142 by binding to the E-box close to the MYB motif on the promoter. Altered expression of these transcription factors highlights that a tight, precise, and coordinated regulation among them is essential for normal pollen development. Most notably, we show that the regulatory pathways of GAMYB and UDT1 rely on bHLH142 in a direct and indirect manner, respectively, and function in different tissues with distinct biological roles during pollen development. This study advances our understanding of the molecular mechanisms of rice pollen development.