bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic response to a major hurricane in Anolis lizards in urban and forest habitats
Authors: Avilés-Rodríguez Kevin J , Winchell Kristin M , De León Luis F , Revell Liam J 
Published: March 2021

Abstract
Little is known about the synergistic impacts of urbanization and hurricanes on synanthropes. We compared morphological traits of the lizard Anolis cristatellus on Puerto Rico sampled before the 2017 category 5 Hurricane Maria and 4 and 11 months after the hurricane. We measured limb lengths, toepad size and the number of subdigital scales, termed lamellae, that facilitate adhesion. We hypothesized that the hurricane should have selected for longer limbs and larger toepads with more lamellae, which are traits that other research has suggested to increase clinging performance. Given prior work demonstrating that urban lizards of this species tend to share this phenotype, we also predicted increased phenotypic overlap between post-hurricane urban–forest pairs. Instead, we found that forest and urban populations alike had smaller body sizes, along with a small size-adjusted decrease in most traits, at 4 months after the hurricane event. Many traits returned to prehurricane values by 11 months post-hurricane. Toe morphology differed in the response to the hurricane between urban and forest populations, with significantly decreased trait values in forest but not in urban populations. This difference could be attributable to the different biomechanical demands of adhesion to anthropogenic substrates compared with natural substrates during intense winds. Overall, more research will be required to understand the impacts of hurricanes on urban species and whether differential natural selection can result.
Private active cyber defense and (international) cyber security—pushing the line?
Authors: Broeders Dennis 
Published: March 2021

Abstract
Private sector Active Cyber Defence (ACD) lies on the intersection of domestic security and international security and is a recurring subject, often under the more provocative flag of ‘hack back’, in the American debate about cyber security. This article looks at the theory and practice of private cyber security provision and analyses in more detail a number of recent reports and publications on ACD by Washington DC based commissions and think tanks. Many of these propose legalizing forms of active cyber defence, in which private cyber security companies would be allowed to operate beyond their own, or their clients’ networks, and push beyond American law as it currently stands. Generally, public-private governance solutions for security problems have to manage a balance between (i) questions of capacity and assigning responsibilities, (ii) the political legitimacy of public–private security solutions and (iii) the mitigation of their external effects. The case of private active cyber defence reveals a strong emphasis on addressing the domestic security (and political) problem, while failing to convincingly address the international security problems. The proposals aim to create a legitimate market for active cyber defence, anchored to the state through regulation and certification as a way to balance capacity, responsibilities and domestic political legitimacy. A major problem is that even though these reports anticipate international repercussions and political pushback, against what is likely be received internationally as an escalatory and provocative policy, they offer little to mitigate it.
Prognostic value of right ventricular dilatation in patients with COVID-19: a multicentre study
Authors: Soulat-Dufour Laurie , Fauvel Charles , Weizman Orianne , Barbe Thomas , Pezel Théo , Mika Delphine , Cellier Joffrey , Geneste Laura , Panagides Vassili , Marsou Wassima , Deney Antoine , Attou Sabir , Delmotte Thomas , Ribeyrolles Sophie , Chemaly Pascale , Karsenty Clement , Giordano Gauthier , Gautier Alexandre , Duceau Baptiste , Sutter Willy , Chaumont Corentin , Guilleminot Pierre , Sagnard Audrey , Pastier Julie , Trimaille Antonin , Bonnet Guillaume , Canu Marjorie , Coisne Augustin , Cohen Ariel 
Published: March 2021

Abstract

Aims
Although cardiac involvement has prognostic significance in coronavirus disease 2019 (COVID-19) and is associated with severe forms, few studies have explored the prognostic role of transthoracic echocardiography (TTE). We investigated the link between TTE parameters and prognosis in COVID-19.

Methods and results
Consecutive patients with COVID-19 admitted to 24 French hospitals were retrospectively included. Comprehensive data, including clinical and biological parameters, were recorded at admission. Focused TTE was performed during hospitalization, according to clinical indication. Patients were followed for a primary composite outcome of death or transfer to intensive care unit (ICU) during hospitalization. Among 2878 patients, 445 (15%) underwent TTE. Most of these had cardiovascular risk factors, a history of cardiovascular disease, and were on cardiovascular treatments. Dilatation and dysfunction were observed in, respectively, 12% (48/412) and 23% (102/442) of patients for the left ventricle, and in 12% (47/407) and 16% (65/402) for the right ventricle (RV). Primary composite outcome occurred in 44% (n = 196) of patients [9% (n = 42) for death without ICU transfer and 35% (n = 154) for admission to ICU]. RV dilatation was the only TTE parameter associated with the primary outcome. After adjustment, male sex [hazard ratio (HR) 1.56, 95% confidence interval (CI) 1.09 − 2.25; P = 0.02], higher body mass index (HR 1.10, 95% CI 1.02 − 1.18; P = 0.01), anticoagulation (HR 0.53, 95% CI 0.33 − 0.86; P = 0.01), and RV dilatation (HR 1.66, 95% CI 1.05 − 2.64; P = 0.03) remained independently associated with the primary outcome.

Conclusion
Echocardiographic evaluation of RV dilatation could be useful for assessing risk of severe COVID-19 developing in hospitalized patients.
Influence of low-level-laser therapy on the stability of orthodontic mini-screw implants. A systematic review and meta-analysis
Authors: Michelogiannakis Dimitrios , Jabr Luay , Barmak Abdul Basir , Rossouw Paul Emile , Kotsailidi Elli Anna , Javed Fawad 
Published: March 2021

Summary

Background
The influence of low-level-laser therapy (LLLT) on the stability of orthodontic mini-screw implants (MSIs) has not been systematically reviewed.

Objectives
The aim was to assess the influence of LLLT on the stability of orthodontic MSIs.

Methods
An unrestricted search of indexed databases was performed.

Selection criteria
Randomized controlled clinical trials (RCTs) investigating the influence of LLLT on orthodontic MSI stability.

Data collection and analysis
Two authors independently performed study retrieval and selection, and data extraction. The risk of bias (RoB) of individual studies was assessed using the Cochrane RoB Tool for RCTs. Meta-analyses were performed separately for RCTs using periotest and resonance frequency analysis (RFA) to measure MSI stability; and a random effects model was applied. Subgroup analyses were performed based on the time-points of MSI stability evaluation. The quality of available evidence was evaluated using the Grades of Recommendation, Assessment, Development, and Evaluation approach.

Results
Initially, 1332 articles were screened. Six RCTs with a split-mouth design were included. The periotest was used in 4 RCTs and 2 RCTs used RFA to measure MSI stability. All RCTs had a low RoB. Subgroup analyses based on periotest indicated that MSIs treated with LLLT had significantly higher stability than untreated MSIs at 21 and 30 days [weighted mean difference (MD) = −2.76, confidence interval (CI): [−4.17, −1.36], P-value = 0.0001) and at 60 days (weighted MD = −3.47, CI: [−4.58, −2.36], P &lt; 0.00001); and the level of certainty was high. Subgroup analyses based on RFA showed higher stability of MSIs treated with than without LLLT at 56 and 60 days (standardized MD = 0.82, CI: [0.32, 1.32], P = 0.001), and at 70 and 90 days (standardized MD = 0.86, CI: [0.36, 1.36], P = 0.0007); and the level of certainty was moderate.

Limitations
Due to limited number of relevant studies, it was not possible to perform sensitivity analysis, subgroup analyses for patient and intervention-related characteristics, and reporting biases assessment.

Conclusions
The role of LLLT on the secondary stability of MSIs placed in patients undergoing OT remains debatable.

Clinical Trial Registration
PROSPERO (CRD42021230291).
Bacterial communities are more sensitive to ocean acidification than fungal communities in estuarine sediments
Authors: Su Xiaoxuan , Yang Xiaoru , Li Hu , Wang Hongtao , Wang Yingmu , Xu Jianxin , Ding Kai , Zhu Yong-guan 
Published: March 2021

ABSTRACT
Ocean acidification (OA) in estuaries is becoming a global concern, and may affect microbial characteristics in estuarine sediments. Bacterial communities in response to acidification in this habitat have been well discussed; however, knowledge about how fungal communities respond to OA remains poorly understood. Here, we explored the effects of acidification on bacterial and fungal activities, structures and functions in estuarine sediments during a 50-day incubation experiment. Under acidified conditions, activities of three extracellular enzymes related to nutrient cycling were inhibited and basal respiration rates were decreased. Acidification significantly altered bacterial communities and their interactions, while weak alkalization had a minor impact on fungal communities. We distinguished pH-sensitive/tolerant bacteria and fungi in estuarine sediments, and found that only pH-sensitive/tolerant bacteria had strong correlations with sediment basal respiration activity. FUNGuild analysis indicated that animal pathogen abundances in sediment were greatly increased by acidification, while plant pathogens were unaffected. High-throughput quantitative PCR-based SmartChip analysis suggested that the nutrient cycling-related multifunctionality of sediments was reduced under acidified conditions. Most functional genes associated with nutrient cycling were identified in bacterial communities and their relative abundances were decreased by acidification. These new findings highlight that acidification in estuarine regions affects bacterial and fungal communities differently, increases potential pathogens and disrupts bacteria-mediated nutrient cycling.
Assessing evidence for adaptive evolution in two hearing-related genes important for high-frequency hearing in echolocating mammals
Authors: Wang Hui , Zhao Hanbo , Chu Yujia , Feng Jiang , Sun Keping 
Published: March 2021

Abstract
High-frequency hearing is particularly important for echolocating bats and toothed whales. Previously, studies of the hearing-related genes Prestin, KCNQ4, and TMC1 documented that adaptive evolution of high-frequency hearing has taken place in echolocating bats and toothed whales. In this study, we present two additional candidate hearing-related genes, Shh and SK2, that may also have contributed to the evolution of echolocation in mammals. Shh is a member of the vertebrate Hedgehog gene family and is required in the specification of the mammalian cochlea. SK2 is expressed in both inner and outer hair cells, and it plays an important role in the auditory system. The coding region sequences of Shh and SK2 were obtained from a wide range of mammals with and without echolocating ability. The topologies of phylogenetic trees constructed using Shh and SK2 were different; however, multiple molecular evolutionary analyses showed that those two genes experienced different selective pressures in echolocating bats and toothed whales compared to nonecholocating mammals. In addition, several nominally significant positively selected sites were detected in the nonfunctional domain of the SK2 gene, indicating that different selective pressures were acting on different parts of the SK2 gene. This study has expanded our knowledge of the adaptive evolution of high-frequency hearing in echolocating mammals.
Genome-wide RNAi screen for regulators of UPRmt in Caenorhabditis elegans mutants with defects in mitochondrial fusion
Authors: Haeussler Simon , Yeroslaviz Assa , Rolland Stéphane G , Luehr Sebastian , Lambie Eric J , Conradt Barbara 
Published: March 2021

Abstract
Mitochondrial dynamics plays an important role in mitochondrial quality control and the adaptation of metabolic activity in response to environmental changes. The disruption of mitochondrial dynamics has detrimental consequences for mitochondrial and cellular homeostasis and leads to the activation of the mitochondrial unfolded protein response (UPRmt), a quality control mechanism that adjusts cellular metabolism and restores homeostasis. To identify genes involved in the induction of UPRmt in response to a block in mitochondrial fusion, we performed a genome-wide RNAi screen in Caenorhabditis elegans mutants lacking the gene fzo-1, which encodes the ortholog of mammalian Mitofusin, and identified 299 suppressors and 86 enhancers. Approximately 90% of these 385 genes are conserved in humans, and one-third of the conserved genes have been implicated in human disease. Furthermore, many have roles in developmental processes, which suggests that mitochondrial function and their response to stress are defined during development and maintained throughout life. Our dataset primarily contains mitochondrial enhancers and non-mitochondrial suppressors of UPRmt, indicating that the maintenance of mitochondrial homeostasis has evolved as a critical cellular function, which, when disrupted, can be compensated for by many different cellular processes. Analysis of the subsets “non-mitochondrial enhancers” and “mitochondrial suppressors” suggests that organellar contact sites, especially between the ER and mitochondria, are of importance for mitochondrial homeostasis. In addition, we identified several genes involved in IP3 signaling that modulate UPRmt in fzo-1 mutants and found a potential link between pre-mRNA splicing and UPRmt activation.
A selection-based next generation sequencing approach to develop robust, genotype-specific mutation profiles in Saccharomyces cerevisiae
Authors: Lamb Natalie A , Bard Jonathan E , Buck Michael J , Surtees Jennifer A 
Published: March 2021

Abstract
Distinct mutation signatures arise from environmental exposures and/or from defects in metabolic pathways that promote genome stability. The presence of a particular mutation signature can therefore predict the underlying mechanism of mutagenesis. These insults to the genome often alter dNTP pools, which itself impacts replication fidelity. Therefore, the impact of altered dNTP pools should be considered when making mechanistic predictions based on mutation signatures. We developed a targeted deep-sequencing approach on the CAN1 gene in Saccharomyces cerevisiae to define information-rich mutational profiles associated with distinct rnr1 backgrounds. Mutations in the activity and selectivity sites of rnr1 lead to elevated and/or unbalanced dNTP levels, which compromises replication fidelity and increases mutation rates. The mutation spectra of rnr1Y285F and rnr1Y285A alleles were characterized previously; our analysis was consistent with this prior work but the sequencing depth achieved in our study allowed a significantly more robust and nuanced computational analysis of the variants observed, generating profiles that integrated information about mutation spectra, position effects, and sequence context. This approach revealed previously unidentified, genotype-specific mutation profiles in the presence of even modest changes in dNTP pools. Furthermore, we identified broader sequence contexts and nucleotide motifs that influenced variant profiles in different rnr1 backgrounds, which allowed specific mechanistic predictions about the impact of altered dNTP pools on replication fidelity.
Natural History and Risk Stratification of Recurrent Crohn’s Disease After Ileocolonic Resection: A Multicenter Retrospective Cohort Study
Authors: Joustra Vincent , Duijvestein Marjolijn , Mookhoek Aart , Bemelman Willem , Buskens Christianne , Koželj Matic , Novak Gregor , Hindryckx Pieter , Mostafavi Nahid , D’Haens Geert 
Published: March 2021

Abstract

Background
Prediction of endoscopic postoperative recurrence (POR) and prophylactic treatment based on clinical risk profile have thus far been inconclusive. This study aimed to examine the association between clinical risk profile and the development of endoscopic POR in a Crohn’s disease population without postoperative treatment and to identify individual risk factors of endoscopic POR.

Methods
Medical records of 142 patients with Crohn’s disease during follow-up after ileocecal or ileocolonic resection without prophylactic treatment at 3 referral centers were reviewed. Endoscopic POR was defined as a modified Rutgeerts score ≥i2b. Clinical risk profiles were distilled from current guidelines. Both uni- and multivariate logistic regression analysis were used to assess the relationship between risk profiles and endoscopic POR.

Results
Endoscopic POR was observed in 68 out of 142 (47.9%) patients. Active smoking postsurgery (odds ratio [OR], 3.01; 95% confidence interval [CI], 1.24-7.34; P = 0.02), a Montreal classification of A3 (OR, 3.05; 95% CI, 1.07-8.69; P = 0.04), and previous bowel resections (OR, 2.58; 95% CI, 1.07-6.22; P = 0.03) were significantly associated with endoscopic POR. No significant association was observed between endoscopic POR and any guideline defined as a high-/low-risk profile. However, patients with a combination of any 3 or more European Crohns & Colitis Organisation– (OR, 4.87; 95% CI, 1.30-18.29; P = 0.02) or British Society of Gastroenterology–defined (OR 3.16; 95% CI, 1.05-9.49; P = 0.04) risk factors showed increased odds of developing endoscopic POR.

Conclusions
Our results suggest that patients with a combination of any 3 or more European Crohns & Colitis Organisation– or British Society of Gastroenterology–defined risk factors would probably benefit from immediate prophylactic treatment.
Nonhypoalbuminemic Inflammatory Bowel Disease in Dogs as Disease Model
Authors: Hernandez Juan , Rouillé Elodie , Chocteau Florian , Allard Marie , Haurogné Karine , Lezin Florence , Hervé Julie M , Bach Jean-Marie , Abadie Jérôme , Lieubeau Blandine 
Published: March 2021

Abstract

Background
The incidence of inflammatory bowel disease (IBD) is increasing worldwide, emphasizing the need of relevant models, as dogs spontaneously affected by IBD may be, for better knowledge of the disease’s physiopathology.

Methods
We studied 22 client-owned dogs suffering from IBD without protein loss and 14 control dogs. Biopsies were obtained from the duodenum, ileum, and colon. Inflammatory grade was assessed by histopathology, immunohistochemistry, and chemokine analysis. The expression of Toll-like receptors (TLR) in mucosa was immunohistochemically evaluated. Antibody levels against bacterial ligands (lipopolysaccharide [LPS] and flagellin) were measured in sera using enzyme-linked immunoassay.

Results
Dogs with IBD showed low to severe clinical disease. Histopathologically, the gut of dogs with IBD did not exhibit significant alterations compared with controls except in the colon. The number of CD3+ T lymphocytes was decreased in the ileum and colon of dogs with IBD compared with controls, whereas the numbers of Foxp3+, CD20+, and CD204+ cells were similar in the 2 groups. Three chemokines, but no cytokines, were detected at the protein level in the mucosa, and the disease poorly affected their tissue concentrations. Dogs with IBD exhibited higher serum reactivity against LPS and flagellin than controls but similar immunoreactivity against the receptors TLR4 and TLR5. In addition, TLR2 and TLR9 showed similar expression patterns in both groups of dogs.

Conclusions
Our data described dysregulated immune responses in dogs affected by IBD without protein loss. Despite fairly homogeneous dog cohorts, we were still faced with interindividual variability, and new studies with larger cohorts are needed to validate the dog as a model.
Reward-Related Responses and Tonic Craving in Cocaine Addiction: An Imaging Study of the Monetary Incentive Delay Task
Authors: Zhornitsky Simon , Dhingra Isha , Le Thang M , Wang Wuyi , Li Chiang-shan R , Zhang Sheng 
Published: March 2021

Abstract

Background
Cocaine addiction is associated with altered sensitivity to natural reinforcers and intense drug craving. However, previous findings on reward-related responses were mixed, and few studies have examined whether reward responses relate to tonic cocaine craving.

Methods
We combined functional magnetic resonance imaging and a monetary incentive delay task to investigate these issues. Imaging data were processed with published routines, and the results were evaluated with a corrected threshold. We compared reward responses of 50 cocaine-dependent individuals (CDs) and 45 healthy controls (HCs) for the ventral striatum (VS) and the whole brain. We also examined the regional responses in association with tonic cocaine craving, as assessed by the Cocaine Craving Questionnaire (CCQ) in CDs. We performed mediation analyses to evaluate the relationship between regional responses, CCQ score, and recent cocaine use.

Results
The VS showed higher activation to large as compared with small or no wins, but this reward-related activity did not differ between CDs and HCs. The precentral gyrus (PCG), anterior insula, and supplementary motor area showed higher activation during large vs no wins in positive correlation with the CCQ score in CDs. Mediation analyses suggested that days of cocaine use in the prior month contributed to higher CCQ scores and, in turn, PCG reward responses.

Conclusions
The results highlight a unique relationship between reward responses of the primary motor cortex, tonic cocaine craving, and recent cocaine use. The motor cortex may partake in the cognitive motor processes critical to drug-seeking behavior in addicted individuals.
Islamic Law and the Balancing of Justice and Peace in Iraq’s Post-IS Landscape
Authors: Mojtahedi Mohammad Hossein , van Wijk Joris 
Published: March 2021

Abstract
Balancing justice and long-term peace and security in a postconflict context is highly complex. This article discusses the challenges in Iraq’s post-IS (Islamic State) landscape. Based on a review of relevant Arabic, English and Persian academic scholarship on Islamic law and Islamic political science on accountability and transitional justice, it discusses what Islamic law dictates state authorities to do with detained suspects of atrocity crimes and terrorism and explores possibilities in Islamic law to balance justice and long-term peace and security. The article concludes that crimes against the state should, according to Islamic law, in principle lead to harsh punishments. However, that same body of law also provides for opportunities to adopt non-retributive alternatives, if this allows government to more swiftly handle the security crisis, re-establish public harmony and prevent further bloodshed in the future. The popular perception of the Iraqi people supporting retributive approaches could however pose an important barrier that could block any changes to Iraq’s current transitional justice approach.