bluuebunny/crossref_metadata_2025_split huggingface dataset:
NNT‐induced tumor cell “slimming” reverses the pro‐carcinogenesis effect of HIF2a in tumors
Authors: Xiong Zhiyong , Xiong Wei , Xiao Wen , Yuan Changfei , Shi Jian , Huang Yu , Wang Cheng , Meng Xiangui , Chen Zhixian , Yang Hongmei , Chen Ke , Zhang Xiaoping 
Published: January 2021

AbstractBackgroundHIF2a and lipid accumulation play key roles in the progression of clear cell renal cell carcinoma (ccRCC). Tumor cell “slimming” is a new concept in which tumor cells with abnormal lipids efficiently consume lipids to inhibit tumor progression without producing additional ATP. However, their respective regulatory mechanisms are still unclear. The purpose of this study is uncovering the links between these three key elements of ccRCC to elucidate new mechanisms of ccRCC metabolic abnormalities and providing a basis for new drug development for ccRCC.MethodsBioinformatics screening and analyses were performed in ccRCC according to TCGA‐KIRC database. qRT‐PCR, luciferase reporter assay, western blot, chromatin immunoprecipitation (ChIP) assays, and other biological methods were used to explore and verify related pathways. Various cell line models and animal models were used to perform related functional experiments.ResultsScreening based on sequencing data after HIF2a knockdown and three independent mitochondrial metabolism‐related gene sets showed that nicotinamide nucleotide transhydrogenase (NNT) was a mediator between HIF2a and tumor cells “slimming.” Further research showed that NNT had significant prognostic predictive value and was downregulated in ccRCC. It is regulated by HIF2a and can significantly activate lipid browning‐mediated tumor cell “slimming.” Mechanistic investigations indicated that HIF2a enhanced the expression of miR‐455‐5p via binding to HIF2a‐related response elements in the miR‐455‐5p promoter, which suppresses NNT expression by binding to its 3′ untranslated region.ConclusionsOur study revealed a novel mechanism by which HIF2a decreased NNT level through a microRNA that suppressed tumor cell “slimming,” resulting in the progression of ccRCC. This mechanism provides a fresh perspective of lipid accumulation in ccRCC and may help target novel strategies for the treatment of tumors with abnormal lipid metabolism.
Reiterative infusions of MSCs improve pediatric osteogenesis imperfecta eliciting a pro‐osteogenic paracrine response: TERCELOI clinical trial
Authors: Infante Arantza , Gener Blanca , Vázquez Miguel , Olivares Nerea , Arrieta Arantza , Grau Gema , Llano Isabel , Madero Luis , Bueno Ana Maria , Sagastizabal Belén , Gerovska Daniela , Araúzo‐Bravo Marcos J , Astigarraga Itziar , Rodríguez Clara I. 
Published: January 2021

AbstractBackgroundOsteogenesis imperfecta (OI) is a rare genetic disease characterized by bone fragility, with a wide range in the severity of clinical manifestations. The majority of cases are due to mutations in the COL1A1 or COL1A2 genes, which encode type I collagen. Mesenchymal stem cells (MSCs), as the progenitors of the osteoblasts, the main type I collagen secreting cell type in the bone, have been proposed and tested as an innovative therapy for OI with promising but transient outcomes.MethodsTo overcome the short‐term effect of MSCs therapy, we performed a phase I clinical trial based on reiterative infusions of histocompatible MSCs, administered in a 2.5‐year period, in two pediatric patients affected by severe and moderate OI. The aim of this study was to assess the safety and effectiveness of this cell therapy in nonimmunosuppressed OI patients. The host response to MSCs was studied by analyzing the sera from OI patients, collected before, during, and after the cell therapy.ResultsWe first demonstrated that the sequential administration of MSCs was safe and improved the bone parameters and quality of life of OI patients along the cell treatment plus 2‐year follow‐up period. Moreover, the study of the mechanism of action indicated that MSCs therapy elicited a pro‐osteogenic paracrine response in patients, especially noticeable in the patient affected by severe OI.ConclusionsOur results demonstrate the feasibility and potential of reiterative MSCs infusion for two pediatric OI and highlight the paracrine response shown by patients as a consequence of MSCs treatment.
An efficient host overload detection algorithm for cloud data center based on exponential weighted moving average
Authors: Kulshrestha Sudhanshu , Patel Sanjeev 
Published: January 2021

SummaryThe major aim of data center (DC) is to optimize the three parameters, viz., energy consumption, resource utilization, and quality of service (QoS). Our objective is to balance these three parameters by managing the virtual machine (VM) efficiently. Therefore, this paper presents a holistic view of VM consolidation and explains its constituent subprocesses: (i) host overload detection, (ii) VM selection for migration from the overloaded host, and (iii) VM placement for provisioning selected VMs on a new set of hosts. Later, this paper attempts to optimize the parameters mentioned above by proposing a host overload detection algorithm based onexponential weighted moving average(EWMA) and its variants. The performance of the proposed EWMA is compared with the state‐of‐the‐art algorithms based on (i) local regression, (ii) median absolute deviation, and (iii) interquartile range. The efficacy of the proposed EWMA is evaluated in combination with four different existing VM selection policies: (i) minimum migration time, (ii) minimum utilization, (iii) maximum correlation, and (iv) random selection. The evaluation metrics comprise energy consumption, VM migration count from the overloaded hosts representing the resource utilization, service level agreement (SLA) violations describing the QoS, and the average execution time. Simulation experiments are carried out on the CloudSim simulator usingPlanetLabreal cloud trace as workload. The proposed EWMA consumes 18.33% less energy, causes 47.81% less VM migration, makes 9.91% less SLA violation, and takes 43.44% less average‐time for the execution of the entire workload as compared to state‐of‐the‐art algorithms.
Retracted: A smart transportation system in VANET based on vehicle geographical tracking and balanced routing protocol
Authors: Kandasamy Satheshkumar , Mangai S. 
Published: January 2021

SummaryIn present scenario, Vehicular Ad‐hoc Network (VANET) has obtained extensive attention because of its unique characteristics that handle high mobility of vehicles, reaping topological changes and frequent route breakages or failures. Hence, for providing smart transportation with enhanced traffic regulation and safety, a new position‐based routing protocol called Traffic Dynamism‐Balanced Routing Protocol (TDBRP) for VANET has been proposed in this paper. Moreover, the work concerns the instantaneous scenario of urban cities with the bidirectional and multilane roads. Efficient junction selection algorithm (EJSA) has been incorporated based on the traffic dynamism for establishing optimal route paths between nodes, hence, forwarding the data packets efficiently. Furthermore, the novel EJSP and the route framing between junctions are established on the basis of information gained from two‐hop neighbors, which tends to reduce end‐to‐end delay and enhances packet delivery ratio (PDR). The TDBRP has been implemented and evaluated in Network Simulator (NS 2.34). And the results are examined and compared with some existing protocols like Geographic Source Routing (GSR), Enhanced Greedy Traffic Aware Routing protocol (E‐GyTAR), and Anchor‐based Street and Traffic‐Aware Routing (A‐STAR). The outcomes outperform the existing works by minimizing the routing overhead and end‐to‐end delay by an average of 16% and 13%, respectively, and increase the PDR value by an average of 25% than others. The proposed protocol also evidences that the two‐hop neighbor information‐based packet forwarding provides better result and efficient routing than neighbor information.
Sea lion optimization algorithm based node deployment strategy in underwater acoustic sensor network
Authors: Kumar Gola Kamal , Chaurasia Nishant , Gupta Bhumika , Singh Niranjan Deepak 
Published: January 2021

SummaryIn the ocean, huge number of sensor nodes (SNs) are located to transfer the information between other nodes using the Underwater Acoustic Sensor Network (UASN) framework. An underwater acoustic communication technique is utilized by this UASN to exchange the information. Because of environmental conditions and adverse channel, the SNs in UASN may have link breakages. Likewise, maximum target coverage rate for SN deployment is considered as another issue. So it is very essential to create a strong communication system in underwater together with the different kind of variations in ocean environment. As a result, the system will perform better data transmission with the severely fluctuating underwater communication conditions. In this paper, a latest optimization algorithm named as Sea Lion Optimization (SLO) procedure is proposed to discover the optimal location for SN in underwater communication. This algorithm optimally places the acoustic SNs based on the maximum connectivity rate by finding the targeted optimal position. The Matlab tool is utilized for implementation purpose, and the different kinds of parameters like connectivity rate, coverage rate, and delay are taken to evaluate the performance of proposed methodology. Moreover, the existing methods like deployment scheme, Connected Dominating set based depth computation Approach (CDA) approach, and distributive approach are taken to contrast the performance of proposed methodology. When compared to the previous algorithms, our proposed methodology achieves 95% connectivity ratio for varying number of acoustic SNs.
Three‐dimensional virtual VR technology in environmental art design
Authors: Wang Yahong , Hu Xiao‐bing 
Published: January 2021

SummaryMore and more diversified environmental art design, combined with advanced 3‐D virtual virtual reality (VR) technology, has made great contributions to the development of modern display design. The purpose of this study is to use the combination of modern technology and traditional art to realize the cohesion of traditional environmental art and promote the economic and cultural development of the city and society. This experiment uses 3D virtual reality virtual technology, which usually takes the form of wearing glasses. There's a screen in front of your eyes. The sound can be transmitted via speakers or acoustics, so that people have an exciting feeling. The application of virtual reality technology to environmental art must follow three principles: the principle of truth in content, the principle of application in form and the principle of the quantity checked. Taking into account the virtual environment art projection function and the degree of user appreciation and referring to the virtual implementation method evaluation indicator in reality, five indices are also formed, such as degree of interaction, degree of photography, degree of function, degree of use, and degree of extension. The experimental results show that the weights of fast and accurate indexes are 0.74 and 0.36, respectively. Virtual interaction technology presents unlimited possibilities, breaks through the narrow nature of traditional display mode, opens up the current real exhibition development space, and provides new thinking for the design of environmental art.
Design and analysis of a novel compact spanner‐shaped ultra‐wideband antenna for MIMO systems
Authors: Addepalli Tathababu , Anitha V.R. 
Published: January 2021

SummaryIn this communication, a novel compact spanner shaped ultra‐wideband (UWB) antenna is designed for multiple input multiple output (MIMO) system applications. The proposed structure of size 24 × 32 mm2 (0.24λ0 × 0.32λ0, λ0 is calculated at lower band frequency) is designed and fabricated on widely available FR4 dielectric material with a dielectric constant of 4.4 and loss tangent of 0.02. The proposed structure comprises two spanner shaped antennas, a defected T‐shaped stub and a pair of small vertical strips. The defected T‐shaped stub and vertical strips are used for getting a complete UWB bandwidth and good isolation between two elements. It is performing from 3.0 to 11.0 GHz with isolation more than 20 dB in most of the operating region. The parametric studies have been done for the required parameters and figured out the surface current distribution at different resonant frequencies within the working region. The MIMO antenna diversity performance is confirmed with low values of envelope correlation coefficient (ECC < 0.005), channel capacity losses (CCL < 0.04 bits/s/Hz), acceptable mean effective gain (MEG ≤ −3 dB, MEG ratio = 0 dB), and total active reflection coefficient (TARC ≤ −10 dB) values. The peak gain values are varying from 1.5 to 6.0 dBi, and the radiation efficiency values are above 83% for the entire operating region. The simulated and measured values are well matched.
Contribution of small tissue biopsy and flow cytometry to preoperative cytological categorization of salivary gland fine needle aspirates according to the Milan System: Single center experience on 287 cases
Authors: Bağlan Tolga , Sak Serpil Dizbay , Ersöz Cevriye Cansız , Ceyhan Koray 
Published: January 2021

AbstractBackgroundMilan system for reporting salivary gland cytopathology (MSRSGC) was proposed to provide a standardized reporting system for salivary gland fine needle aspiration biopsies. Modified Menghini type semi‐automatic aspiration biopsy needles provide small tissue fragments (STFs), as well as cellular smears, and immunohistochemical and molecular studies can be performed using the STFs.AimsWe aimed to evaluate the contribution of STFs and ancillary techniques to pre‐operative diagnosis of salivary gland lesions.Materials and MethodsIn this study, smears of 287 cases with histopathological correlation were re‐reviewed and assigned to one of the MSRSGC categories. In the second step, histopathological and immunohistochemical findings in STFs were evaluated together with cytological findings. Final diagnoses were obtained with the inclusion of flow cytometry (FC) results when available. Risk of malignancy (ROM) was calculated for each diagnostic category.ResultsIn the evaluation based on smears, a specific diagnosis could be obtained in 64.8% of the cases. ROMs were 0% for nondiagnostic (ND), 5.6% for non‐neoplastic (NN), 55% for atypia of undetermined significance (AUS), 0.6% for benign neoplasm (BN), 27.8% for salivary gland neoplasm of uncertain malignant potential (SUMP), and 100% for suspicious for malignancy (SFM) and malignant (M) categories. With the addition of histopathological and immunohistochemical findings and FC results, a specific diagnosis could be obtained in 75.2% of the cases. ROMs were 0% for ND, 4.5% for NN, 53.8% for AUS, 0.6% for BN, 0% for SUMP, and 100% for SFM/M categories.ConclusionsSTFs contribute correct categorization of salivary gland lesions. The major contribution of ancillary methods is in the SUMP category.
HDAC6 inhibitor, ACY1215 suppress the proliferation and induce apoptosis of gallbladder cancer cells and increased the chemotherapy effect of gemcitabine and oxaliplatin
Authors: Ruan Yi , Wang Luoluo , Lu Yeting 
Published: January 2021

AbstractAs an anti‐tumor agent, histone deacetylases (HDACs) inhibitors have attracted wide attention. ACY1215 is a highly effective selective inhibitor of HDAC6, which can inhibit many kinds of tumors. Whether the expression of HDAC6 and its new inhibitor ACY1215 can inhibit the proliferation of gallbladder cancer cells and induce their apoptosis remains to be further studied. The purpose of this study was to explore the effects of ACY1215 on the gallbladder cancer cells. Cell proliferation of GBC‐SD and SGC‐996 was assessed by cell counting kit‐8 assay and colony formation assay. Flow cytometry was used to detect the apoptosis of gallbladder cancer cells. Western blot was used to detect the expressions of PCNA，KI67, and apoptosis‐related proteins of gallbladder cancer cells. The HDAC6 inhibitor ACY1215 suppressed the proliferation of GBC‐SD and SDC‐996 cells and promoted the apoptosis of gallbladder cancer cells. The HDAC6 inhibitor ACY1215 increases the chemotherapy effect of gemcitabine and oxaliplatin. ACY1215 could suppress cell proliferation and induce apoptosis of GBC‐SD and SGC‐996, and increased the chemotherapy effect of gemcitabine and oxaliplatin, which provides a rationale for the combination of HDAC6 selective inhibitors with other anticancer agents in treating gallbladder cancer.
Overview of in situ gelling injectable hydrogels for diabetic wounds
Authors: Mude Lavanya , Sanapalli Bharat Kumar Reddy , V Anoop Narayanan , Singh Sachin Kumar , Karri Veera Venkata Satyanarayana Reddy 
Published: January 2021

AbstractDiabetes mellitus (DM) is an endocrine disorder that causes increased blood glucose than usual due to insulin impairment. In DM, several complications arise in which diabetic wound (DW) is the most devastating complication. About 25% of patients with DM expected to develop DWs in their lifetime and undergo limb amputations. Even though several treatments such as surgery, debridement, wound dressings, advanced therapies were available, the overall conclusion has been that with very few exceptions, patients still suffer from limitations like pain, frequent dress changing, high rates of failure, and cost involvement. Further, the treatments involving the delivery of therapeutic agents in treating DWs have limited success due to abnormal levels of proteases in the DW environment. In this backdrop, in situ gelling injectable hydrogels have gained special attention due to their easy encapsulation of therapeutic medications and prolonged release, filling the wound defect areas, ease of handling, and minimally invasive surgical procedures. Though the in situ gelling injectable hydrogels are developed a couple of decades ago, their use for treating DW has not yet been explored thoroughly. Thus, in this review, we have covered the sequential events of DW healing, pathophysiology, current treatments, and its limitations, along with a particular emphasis on the mechanism of action of these in situ gelling injectable hydrogels treating DWs.
Roles of culture and COMT Val58Met gene on neural basis of executive function: A comparison between Japanese and American children
Authors: Senzaki Sawa , Pott Uwe , Shinohara Ikuko , Moriguchi Yusuke 
Published: January 2021

AbstractThe development of executive functions (EF) is shaped by both genetic and environmental factors, including cultural background. Genetically, variation in the catechol‐O‐methyltransferase (COMT) gene polymorphism has been linked to EF performance and differential regulation of prefrontal cortex activity. Based on the gene–culture interaction framework, we tested whether culture would moderate the association between the COMT gene and young children's behavioral responses and neural activities during a cognitive‐shifting EF task. The children who participated in this study were 5‐ to 6‐year‐olds in Japan (n = 44) and in the U.S. (n = 47). The results revealed that U.S.‐American children exhibited stronger activations than Japanese children in the right dorsolateral and bilateral prefrontal cortex regions. In addition, the children's genetic disposition and EF performance were marginally moderated by culture, with Val homozygote Japanese children performing better than Met‐allele carriers, whereas no such differences were found in U.S.‐American children. We have discussed the theoretical and empirical implications of the construction of a more complete understanding of EF development by incorporating both genetic and socio‐cultural factors.
Single hair analysis: Validation of a screening method for over 150 analytes and application on documented single‐dose cases
Authors: Wiedfeld Christopher , Skopp Gisela , Musshoff Frank 
Published: January 2021

AbstractHair is the matrix of choice in forensic toxicology when retrospective analysis is needed. Nonetheless, due to misalignment, different growth stages and segmentation lengths of 0.5–1 cm, resolution of time is limited. By segmental analysis of single hairs, most of these factors can be compensated and resolution of time is enhanced. A method for manually segmenting single hairs in 2‐mm sections and screening for 156 analytes by liquid chromatography coupled to tandem mass spectrometry has been developed and validated. The method was applied to 15 single‐dose cases concerning different pharmaceuticals by analyzing 10 hairs each, sampled 1 and 2 months after ingestion in most cases. The validation showed a lower limit of quantification of ≤1.25 pg/segment for ~90% of analytes and good accuracy. Many substances could be detected in the presented cases, whereas detection of benzodiazepines and low dosed opioids remains challenging. In positive cases, characteristic peak‐shaped concentration profiles across the hairs were obtained. The segment with most coinciding peak maxima can be allocated to the time of ingestion. A method for the determination of individual hair growth rate was applied and revealed a gap between expected and actual position of peak maxima. Additionally, different localization of simultaneously administered substances was observed. These findings were tried to be explained by different routes of incorporation and may contribute to current knowledge. The presented method may directly be applied to similar questions in hair analysis, and the findings are considered important for interpreting further results in single hair analysis.
Single‐cell analysis of nonhuman primate preimplantation development in comparison to humans and mice
Authors: Hu Youjin , Huang Kevin , Zeng Qiao , Feng Yun , Ke Qiong , An Qin , Qin Lian‐Ju , Cui YuGui , Guo Ying , Zhao Dicheng , Peng Yu , Tian Di , Xia Kun , Chen Yong , Ni Bin , Wang Jinmei , Zhu Xianmin , Wei Lai , Liu Yizhi , Xiang Peng , Liu Jia‐Yin , Xue Zhigang , Fan Guoping 
Published: January 2021

AbstractBackgroundGenetic programs underlying preimplantation development and early lineage segregation are highly conserved across mammals. It has been suggested that nonhuman primates would be better model organisms for human embryogenesis, but a limited number of studies have investigated the monkey preimplantation development. In this study, we collect single cells from cynomolgus monkey preimplantation embryos for transcriptome profiling and compare with single‐cell RNA‐seq data derived from human and mouse embryos.ResultsBy weighted gene‐coexpression network analysis, we found that cynomolgus gene networks have greater conservation with human embryos including a greater number of conserved hub genes than that of mouse embryos. Consistently, we found that early ICM/TE lineage‐segregating genes in monkeys exhibit greater similarity with human when compared to mouse, so are the genes in signaling pathways such as LRP1 and TCF7 involving in WNT pathway. Last, we tested the role of one conserved pre‐EGA hub gene, SIN3A, using a morpholino knockdown of maternal RNA transcripts in monkey embryos followed by single‐cell RNA‐seq. We found that SIN3A knockdown disrupts the gene‐silencing program during the embryonic genome activation transition and results in developmental delay of cynomolgus embryos.ConclusionTaken together, our study provided new insight into evolutionarily conserved and divergent transcriptome dynamics during mammalian preimplantation development.