bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of pen enrichment on leg health of fast and slower-growing broiler chickens
Authors: Güz Bahadır Can , de Jong Ingrid C. , Souza Da Silva Carol , Veldkamp Fleur , Kemp Bas , Molenaar Roos , van den Brand Henry 
Published: July 2021

AbstractPen enrichment for broiler (meat-type) chickens is one of the potential strategies to stimulate locomotion and consequently contribute to leg health and welfare. This study was designed to evaluate effects of using a plethora of pen enrichments (barrier perches, angular ramps, horizontal platforms, large distance between feed and water and providing live Black Soldier fly larvae in a dustbathing area) on tibia characteristics, locomotion, leg health and home pen behaviour of fast and slower-growing broiler chickens. The experiment was set up as a 2 x 2 factorial arrangement with a total of 840 male broiler chickens in a complete randomized design (7 replicates per treatment and 30 chickens per replicate) with the following treatments: 1) pen enrichment (enriched pen or non-enriched pen); 2) broiler strain (fast-growing Ross 308 or slower-growing Hubbard JA 757). Home pen behaviour and use of enrichment were observed. At approximately 1400 and 2200 gram body weight, two chickens per pen were randomly selected and slaughtered, to investigate tibia morphological, biophysical and mechanical characteristics and leg health. Pen enrichment positively affected tibia biophysical characteristics, e.g., osseous volume (Δ=1.8 cm3,P=0.003), total volume (Δ=1.4 cm3,P=0.03) and volume fraction (Δ=0.02 %,P=0.002), in both fast and slower-growing chickens, suggesting that pen enrichment particularly affects ossification and mineralization mechanisms. Accordingly, locomotion and active behaviours were positively influenced by pen enrichment. However, pen enrichment resulted in lower body weight gain in both strains, which might be due to higher activity or lower feed intake as a result of difficulties of crossing the barrier perches. Regarding the strain, slower-growing chickens showed consistently more advanced tibia characteristics and more active behaviour than fast-growing chickens. It can be concluded that pen enrichment may lead to more activity and better bone development in both fast and slower-growing chickens.
A real-time assay for cell-penetrating peptide-mediated delivery of molecular cargos
Authors: Gentry Schuyler B. , Nowak Scott J. , Ni Xuelei , Hill Stephanie A. , Wade Lydia R. , Clark William R. , Keelaghan Aidan P. , Morris Daniel P. , McMurry Jonathan L. 
Published: July 2021

AbstractCell-penetrating peptides (CPPs) are capable of transporting molecules to which they are tethered across cellular membranes. Unsurprisingly, CPPs have attracted attention for their potential drug delivery applications, but several technical hurdles remain to be overcome. Chief among them is the so-called ‘endosomal escape problem,’ i.e. the propensity of CPP-cargo molecules to be endocytosed but remain entrapped in endosomes rather than reaching the cytosol. Previously, a CPP fused to calmodulin that bound calmodulin binding site-containing cargos was shown to efficiently deliver cargos to the cytoplasm, effectively overcoming the endosomal escape problem. The CPP-adaptor, “TAT-CaM,” evinces delivery at nM concentrations and more rapidly than we had previously been able to measure. To better understand the kinetics and mechanism of CPP-adaptor-mediated cargo delivery, a real-time cell penetrating assay was developed in which a flow chamber containing cultured cells was installed on the stage of a confocal microscope to allow for observationab initio. Also examined in this study was an improved CPP-adaptor that utilizes naked mole rat (Heterocephalus glaber) calmodulin in place of human and results in superior internalization, likely due to its lesser net negative charge. Adaptor-cargo complexes were delivered into the flow chamber and fluorescence intensity in the midpoint of baby hamster kidney cells was measured as a function of time. Delivery of 400 nM cargo was observed within seven minutes and fluorescence continued to increase linearly as a function of time. Cargo-only control experiments showed that the minimal uptake which occurred independently of the CPP-adaptor resulted in punctate localization consistent with endosomal entrapment. A distance analysis was performed for cell-penetration experiments in which CPP-adaptor-delivered cargo showing wider dispersions throughout cells as compared to an analogous covalently-bound CPP-cargo. Small molecule endocytosis inhibitors did not have significant effects upon delivery. The real-time assay is an improvement upon static endpoint assays and should be informative in a broad array of applications.
Deficiency inLystfunction leads to accumulation of secreted proteases and predisposition to mechanic stress-induced retinal detachment
Authors: Ji Xiaojie , Zhao Lihong , Umapathy Ankita , Fitzmaurice Bernard , Wang Jieping , Williams David S. , Chang Bo , Naggert Jürgen K. , Nishina Patsy M. 
Published: July 2021

ABSTRACTChediak–Higashi syndrome, caused by mutations in theLysosomeTrafficking Regulator (Lyst) gene, is a recessive hypopigmentation disorder characterized by albinism, neuropathies, neurodegeneration, and defective immune responses, with enlargement of lysosomes and lysosome-related organelles. Although recent studies have suggested thatLystmutations impair the regulation of sizes of lysosome and lysosome-related organelle, the underlying pathogenic mechanism of Chediak–Higashi syndrome is still unclear. Here we show striking evidence that deficiency in LYST protein function leads to accumulation of photoreceptor outer segment phagosomes in retinal pigment epithelial cells, and reduces adhesion between photoreceptor outer segment and retinal pigment epithelial cells in a mouse model of Chediak–Higashi syndrome. In addition, we observe elevated levels of cathepsins, matrix metallopeptidase (MMP) 3 and oxidative stress markers in the retinal pigment epithelium ofLystmutants. Previous reports showed that impaired degradation of photoreceptor outer segment phagosomes causes elevated oxidative stress, which could consequently lead to increases of cysteine cathepsins and MMPs in the extracellular matrix. Taken together, we conclude that the loss of LYST function causes accumulation of phagosomes in the retinal pigment epithelium and elevation of several extracellular matrix-remodeling proteases through oxidative stress, which may, in turn, reduce retinal adhesion. Our work reveals previously unreported pathogenic events in the retinal pigment epithelium caused byLystdeficiency, which may place Chediak–Higashi syndrome patients at increased risk for retinal detachment. The same pathogenic events may be conserved in other professional phagocytic cells, such as macrophages in the immune system, contributing to overall Chediak–Higashi syndrome pathology.
Highly selective Cooperative Primers for multiplex detection of rare mutations
Authors: Kent Jana Oliveriusova , Christensen Masen Chad 
Published: July 2021

AbstractWe describe a real time PCR-based technique capable of detecting and quantifying rare somatic mutations in circulating tumor DNA reference materials. Our approach utilizes previously described Cooperative Primers, structurally modified to exhibit high allele-specificity and one-copy target sensitivity. Cooperative Primers are bi-functional molecules, consisting of a high affinity probe fragment that guarantees sensitivity, and a covalently attached lower affinity primer providing specificity. Additional optimization of Cooperative Primer structure generated molecules capable of reliable detection of allele changes as small as a single nucleotide. These highly selective Cooperative Primers maintain excellent discrimination properties in rare mutant allele scenarios, in both monoplex and multiplex assays. With synthetic DNA samples, Cooperative Primers can detect as little as 100 copies of mutant template amongst 1 000 000 copies of wild-type template (minor allele fraction of 0.01 %). Multiplex Cooperative Primer assay was validated with cell-free DNA reference materials and consistently detected the lowest minor allele fraction available (0.1 %) for EGFR L858R, G719S and V769-D770insASV mutations, while simultaneously providing qualitative and quantitative assessment of cell-free DNA with integrated β-Actin assay. Easy to design, rapid and inexpensive, Cooperative Primer - based real time PCR assays are a promising tool for evaluation of cancer therapy response, occurrence of resistance mutations and relapse monitoring.
Bioerosion traces and borings in the Upper Devonian vertebrate remains from the Holy Cross Mountains, Poland Bioerosion in fish remains
Authors: Szrek Piotr , Dworczak Patrycja G. , Wilk Olga 
Published: July 2021

AbstractAmong the hundreds of collected Devonian vertebrate macrofossils in the Holy Cross Mountains, placoderms dominate and provide data on their morphology, distribution and taphonomy. So far 17 out of more than 500 studied specimens have revealed bones with surfaces covered by sediment-filled trace fossils. The traces have been made on the vertebrate remains before their final burial. The borings, oval in cross-section, include dendroidal networks of shallow tunnels or short, straight or curved individual scratches and grooves, which frequently create groups on the both sides of the bones. ?Karethraichnusisp. from Kowala and ?Osteocallisisp. from Wietrznia are the oldest record of these ichnogenera. Sedimentological clues indicate a shallow water environment, probably from the slope below the storm wave base.
Waves and currents decrease the available space in a salmon cage
Authors: Johannesen Ása , Patursson Øystein , Kristmundsson Jóhannus , Dam Signar Pætursonur , Mulelid Mats , Klebert Pascal 
Published: July 2021

AbstractDue to increasing demand for salmon and environmental barriers preventing expansion in established sites, salmon farmers seek to move or expand their production to more exposed sites. In this study we investigate the effects of strong currents and waves on the behaviour of salmon and how they choose to use the space available to them. Observations are carried out in a site with strong tidal currents and well mixed water. Using video cameras and echo sounders, we show that salmon prefer to use the entire water column, narrowing their range only as a response to cage deformation, waves, or daylight. Conversely, salmon show strong horizontal preference, mostly occupying the portions of the cage exposed to currents. Additionally, waves cause salmon to disperse from the exposed side of the cage to the more sheltered side. Even when strong currents decrease the amount of available space, salmon choose to occupy the more exposed part of the cage. This indicates that at least with good water exchange, the high density caused by limited vertical space is not so aversive that salmon choose to move to less desirable areas of the cage. However, the dispersal throughout the entire available water column indicates that ensuring enough vertical space, even in strong currents, would be beneficial to salmon welfare.
Targeting “Immunogenic Hotspots” in Dengue and Zika Virus: A Novel Approach to a Common Vaccine
Authors: Mahata Dhrubajyoti , Mukherjee Debangshu , Malviya Vanshika , Mukherjee Gayatri 
Published: July 2021

AbstractDiseases caused by Dengue (DENV) and Zika (ZIKV) viruses cause significant mortality and illness globally. Due to the high sequence similarity of the viral proteins and the purported cross-reactive immune responses against the viruses, we envisioned a common multi-epitope vaccine (MEV) against both viruses by adopting a novel approach of identifying “immunogenic hotspots”. These stretches of the structural and non-structural proteins are enriched with MHC class I and class II supertype-restricted T cell epitopes, and B cell epitopes, in addition to being highly conserved between different DENV serotypes and ZIKV. Such an approach ensures inclusion of multiple overlapping T and B cell epitopes common to both viruses, and also warrants high population coverage. Importantly, epitopes known to cause antibody-dependent-enhancement of infection have been excluded. These immunogenic hotspots have then been stitched together with linkers in-silico along with an adjuvant, CTxB to develop the MEV candidate. Four structural models of the MEV were selected on the basis of conformational preservation of CTxB, and their biophysical parameters, which also conserved the immunogenicity of the multiple epitopes. Importantly, each of the MEV candidates were found to interact with TLR4-MD2 complex by molecular docking studies, indicative of their ability to induce TLR-mediated immune responses.
Transcriptome and metabolome analyses reveal pathways associated with fruit color in plum (Prunus salicina Lindl.)
Authors: Chen Lei , Wang XueSong , Cui Long , Zhang YanBo 
Published: July 2021

AbstractBackgroundIn order to reveal the mechanism of fruit color changes in plum, two common plum cultivars Changli84 (Ch84, red fruit) and Dahuangganhe (D, yellow fruit) in Northeast China were selected as plant materials. Transcriptome sequencing and metabonomic analyzing were performed at three different developmental stages: young fruit stage, colour-change stage, and maturation stage.Results“Flavonoid biosynthesis” was significantly enriched in the KEGG analysis. Some DEGs in “Flavonoid biosynthesis” pathway had an opposite trend between the two cultivars, such as CHS, DFR and FLS. Also, transcriptional control of MBW (MYB–bHLH–WD) protein complexes showed a close relationship with plum fruit color, especially the expression of MYBs and bHLHs. In the current study, procyanidin B1 and B2 had the highest level at young fruit stage in Ch84 and the content of procyanidin B2 decreased sharply at the color change stage. Conversely, the content of cyanidin increased with the growth of fruit and reached the peak at the maturation stage.ConclusionThe content of procyanidin B1 and B2 in plums at young fruit stage might be the leading factors of the matured fruit color. At the maturation stage, the cyanidin produced by procyanidins keeps the color of the fruit red. Correspondingly, genes in “flavonoid biosynthesis” pathway play critical roles in regulating the accumulation of anthocyanin in plum.
Life history of Dactylopius opuntiae (Hemiptera: Dactylopiidae) on Moroccan resistant cactus germplasm
Authors: Aalaoui Mohamed El , Sbaghi Mohamed 
Published: July 2021

AbstractThe important damages caused by Dactylopius opuntiae (Hemiptera: Dactylopiidae) to cactus crops around the world require an integrated pest management (IPM) approach, based on the combination of several techniques (varietal resistance, biological, chemical methods, etc). In this sense, this study evaluated the resistance of 10 Moroccan cactus genotypes to D. opuntiae in order to characterize the expression of antixenosis and/or antibiosis. Antixenosis was accessed in the greenhouse and in the laboratory (26± 2°C) using choice and non-choice tests with 1st instar nymphs. Aakria and Cherratia showed a strong antixenosis effect towards D. opuntiae (0-0.3 D. opuntiae alive 30 after infestation). For antibiosis assessment, 30 1st instar nymphs were confined on cladodes of the 10 selected genotypes under the same laboratory conditions to allow their development, as well as the life cycle performance and behavior of D. opuntiae on the 10 selected cactus genotypes, were evaluated under greenhouse conditions. No influence of genotypes on insect oviposition was observed, indicating that the mealybug does not prefer any genotypes over the others for oviposition. The mealybug failed to develop on genotypes Aakria and Cherratia and did not grow beyond the young female stage on all other resistant genotypes tested. Similarly, first instar nymphs fed on genotypes Marjana, Melk Zhar, and A200 died without reaching the second instar nymph stage. In addition, all genotypes tested had a negative effect on nymph viability (<24%), indicating resistance (antibiosis and/or antixenosis) to the cactus scale. These cactus genotypes may all be useful in breeding programs focused on cactus resistance to mealybugs.
Effective presence of antibodies against common human coronavirus in IgG immunoglobulin medicinal products
Authors: Díez José María , Romero Carolina , Gajardo Rodrigo 
Published: July 2021

AbstractIntroductionIn this series of studies, immunoglobulin products (IgG) formulated for different routes of administration (IV, IM, SC) and prepared from geographically diverse plasma pools were tested for activity against common human coronaviruses (HCoV). IgG products from plasma obtained from Germany, Czech Republic, Slovak Republic, USA and Spain were tested for antibodies to four common HCoV: 229E, OC43, NL63 and HKU1. Since these products are manufactured from pooled plasma from thousands of donors, the antibodies therein are a representation of the HCoV exposure of the population at large.MethodsIgG products of different concentrations manufactured from geographically diverse plasma pools were tested for antibodies to four common HCoV by ELISA. In addition, neutralization assays were conducted using HCoV-229E expressed in MRC5 cells. Complete concentration-neutralization curves were obtained to calculate potencies.ResultsThe ELISA assays showed that when expressed as specific activity (anti-HCoV activity/mg IgG) similar activity against the four common HCoV was seen across the IgG products regardless of concentration or geographic origin. Highest anti-HCoV activity was seen against HCoV-229E, followed by HCoV-OC43 and then HCoV-NL63 and HCoV-HKU1. The neutralization assays showed similar potency for two preparations of IgG prepared by different processes.ConclusionsThese studies are the first demonstration of antibodies to common HCoV in IgG products. The level of activity was similar regardless of the geographic origin of the plasma pool. These antibodies demonstrated neutralization activity against HCoV-229E in MRC5 cells. These results may explain the cross-reactivity seen with pre-pandemic IgG products and SARS-CoV-2 and contribute to the variability in disease course in different patients.
A typology of horizontal logistics collaboration concepts: an illustrative case study from agri-food supply chains
Authors: Badraoui Ismail , Boulaksil Youssef , Van der Vorst Jack G.A.J. 
Published: July 2021

PurposeThe purpose of this paper is to develop a comprehensive model for horizontal logistics collaboration (HLC), including the collaboration types, enablers, context influence and performance indicators.Design/methodology/approachFirst, this study discusses the currently available typologies and their limitations and defines relevant collaboration classification dimensions. Then, a detailed analysis of each dimension is conducted, including the identification of resulting collaboration types. Next, collaboration enablers and the context influence are discussed, as well as their implications on the logistics system, with a specific focus on agri-food supply chains (AFSCs). Additionally, adequate key performance indicators (KPIs) are selected to evaluate collaboration outcomes. Finally, the horizontal logistics collaboration concept (HLCC) is applied to an illustrative case study from AFSCs.FindingsThe results show that HLC is a complex strategy where several elements intervene in the creation of the collaboration scenario. The research also shows that the specific characteristics of AFSCs influence the partners' selection process and increase the importance of partners' similarity and information exchange.Practical implicationsThe results provide managers with practical insights into the dynamic nature of HLC both at the operational and relational levels.Originality/valueThis paper provides a theoretical contribution by introducing a new comprehensive model for HLC and a practical typology that allows a deeper understanding of the mechanisms governing different HLC scenarios.
Smart supply chain and firm performance: the role of digital technologies
Authors: AlMulhim Abdullah Fahad 
Published: July 2021

PurposeThis study aims to explore the impact of digital transformation on firm performance with the help of smart technologies.Design/methodology/approachThis study used a valid and well-structured survey to collect data from 460 respondents from 150 Small and medium-sized enterprises (SMEs) in Saudi Arabia. In order to analyze this data, SmartPLS software was used.FindingsThe empirical results of this study found that the digital transformation has no significant direct impact on firm performance. Therefore, in this situation, smart technologies play an important role in developing the link between digital transformation and firm performance.Originality/valueThis study contributes to the digital supply chain from SMEs' perspective by investigating the mediating role of smart technologies between digital transformation and firm performance.
A scientometric review of construction progress monitoring studies
Authors: Patel Tirth , Guo Brian H.W. , Zou Yang 
Published: July 2021

PurposeThis article aims to explore valuable insights into the construction progress monitoring (CPM) research domain, which include main research topics, knowledge gaps and future research themes. For a long time, CPM has been significantly researched with increasing enthusiasm. Although a few review studies have been carried out, there is non-existence of a quantitative review study that can deliver a holistic picture of CPM.Design/methodology/approachThe science mapping-based scientometric analysis was systematically processed with 1,835 CPM-related journal articles retrieved from Scopus. The co-authorship analysis and direct citation analysis were carried out to identify the most influential researchers, countries and publishers of the knowledge domain. The co-occurrence analysis of keyword was assessed to reveal the most dominating research topics and research trend with the visual representation of the considered research domain.FindingsThis study reveals seven clusters of main research topics from the keyword co-occurrence analysis. The evolution of research confirms that CPM-related research studies were mainly focused on fundamental and traditional CPM research topics before 2007. The period between 2007 and 2020 has seen a shift of research efforts towards digitalization and automation. The result suggests Building Information Modelling (BIM) as the most common, growing and influential research topic in the CPM research domain. It has been used in combination with different data acquisition technologies (e.g. photogrammetry, videogrammetry, laser scanning, Internet of Things (IoT) sensors) and data analytics approaches (e.g. machine learning and computer vision).Practical implicationsThis study provides the horizon of potential research in the research domain of CPM to researchers, policymakers and practitioners by availing of main research themes, current knowledge gaps and future research directions.Originality/valueThis paper represents the first scientometric study depicting the state-of-the-art of the research by assessing the current knowledge domain of CPM.