bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a Multiplex Droplet Digital PCR Assay for Detection of Enterovirus, Parechovirus, Herpes Simplex Virus 1 and 2 Simultaneously for Diagnosis of Viral CNS Infections
Authors: Zhu Xunhua , Liu Pengcheng , Lu Lijuan , Zhong Huaqing , Xu Menghua , Ja Ran , Su Liyun , Cao Lingfeng , Sun Yameng , Guo Meijun , Sun Jianyue , Xu Jin 
Published: December 2021

Abstract
Background: Enterovirus (EV), parechovirus (HPeV), herpes simplex virus 1 and 2 (HSV1/2) are common viruses leading to viral central nervous system (CNS) infections which are increasingly predominant but exhibit deficiency in definite pathogen diagnosis with gold-standard quantitative PCR method. Previous studies have shown that droplet digital PCR (ddPCR) has great potential in pathogen detection and quantification especially in low concentration samples.Methods: Targeting four common viruses of EV, HPeV, HSV1, and HSV2 in cerebrospinal fluid (CSF), we developed a multiplex ddPCR assay using probe ratio-based multiplexing strategy, analyzed the performance, and evaluated it in 97 CSF samples collected from patients with suspected viral CNS infections on a two-channel ddPCR detection system.Results: The four viruses were clearly distinguished by their corresponding fluorescence amplitude. The limits of detection for EV, HPeV, HSV1, and HSV2 were 5, 10, 5, and 10 copies per reaction, respectively. The dynamic range was at least four orders of magnitude spanned from 2000 to 2 copies per reaction. The results of 97 tested clinical CSF specimens were identical to those deduced from qPCR/qRT-PCR assays using commercial kits.Conclusion: The multiplex ddPCR assay was demonstrated to be an accurate and robust method which could detect EV, HPeV, HSV1, and HSV2 simultaneously. It provides a useful tool for clinical diagnosis and disease monitoring of viral CNS infections.
Comparative Study of the Electrical, Thermal and Mechanical properties of Polypyrrole/CB/and Copper nano powder reinforced Polyester Hybrid Composites
Authors: Nosheen Sumaira , Abidi Syed Hussain , Syed Quratulain , Irfan Muhammad , Habib Farzana , Soomro Badaruddin 
Published: December 2021

Abstract
The present research work based on Synthesis and characterization of Polypyrrole coated CB (carbon black), Copper, and VGCNF (vapor grown carbon nanofibers) hybrid composite. Two approaches were used comprising in situ polymerization of Polypyrrole/CB composite. The resulting composite was dispersed in with the measured quantity of Cu nano Powder and VGCNF in polyester at very high rate of dispersion to produced hybrid composite. The resulting composites were characterized by FTIR, electrical conductivity, mechanical properties, thermal analysis and UV visible spectroscopy.
Health Related Quality of Life and its Associated Factors Among Adults Living with Asthma Attending at Selected Public Hospitals in North-Western, Ethiopia: A Multi-Centered Cross- Sectional Study
Authors: Belachew Eyayaw Ashete , Tadess Sumeya , Alemayehu Mekuriaw 
Published: December 2021

Abstract
Background: Asthma is a major public health challenge with most of the patients are not diagnosed and treated appropriately. Health related quality of life is an important measure of health outcomes of asthmatic patient that reflects the impact of an illness and its treatment from the patient’s perspective. Hence, the aim of this study was to assess the health-related quality of life and its determinants among asthmatic patient at selected public hospitals in northwestern, EthiopiaMethod: A multicenter facility based cross-sectional study was conducted in North-Western Ethiopia, from May to July, 2021. A systematic random sampling technique was employed to include 409 study participants. Simple and multivariable linear regressions were conducted to determine variables that are the independent predictors of health-related quality of life. A p-value of <0.05 was considered statistically significant.Result: A total of 409 patients were included in the final analysis and more than half (59.2%) of subjects had good quality of life. Concerning to determinants of health-related quality life, asthma control score (β=0.14, P<0.001), insurance user (β=0.15, P=0.04), high role of patient enablement (β= 0.39, P<0.001), belief to asthma medication (β= (-0.23), P=0.001), health care provides non-adherence to guideline (β= (-0.30), P<0.001) and being house wife (β= (-0.21) P=0.04) were some of significant predictor of health-related quality of life of asthmatic patient.Conclusion: The health-related quality of life among adults with asthma was largely dependent to the level of asthma control. Socio- demographic, clinical, health care related and medication related, variables were significantly health related quality of life. Therefore, our study highlights multifaced interventions including comprehensive asthma education along with an integrated treatment plan to improve asthma control and quality of life.
Clinical Experience and Management Strategy of Retroperitoneal Tumor With Venous Tumor Thrombus Involvement
Authors: Liu Zhuo , Ge Liyuan , Liu Lei , Zhao Xun , Chen Kewei , Li Yuxuan , Aili Abudureyimujiang , Lu Min , Pei Xinlong , Han Dengyang , Zhang Shudong , Ma Lulin 
Published: December 2021

Abstract
Background: To report the surgical management, complications, and outcomes for patients with retroperitoneal tumor and venous thrombus Methods: We retrospectively analyzed 19 cases of retroperitoneal tumor with venous tumor thrombus from August 2015 to March 2021. According to the characteristics of surgical techniques, a new tumor thrombus PUTH grading system was proposed. Results: There were 2 cases of PUTH-1a, 2 cases of PUTH-1b, 6 cases of PUTH-2, 6 cases of PUTH-3,3 cases of PUTH-4. All the operations were successfully performed in 19 patients. Among them, 5 cases (26.3%) were operated by completely laparoscopic approach and 13 cases (68.4%) were operated by open approach. One case (5.3%) was converted from laparoscopic approach to open approach. 5 cases experienced postoperative complications, the incidence was 26.3%. All the cases were followed-up for a median of 14 months. Cancer associated death were occurred in 3 cases. Distant metastases were occurred in 7 cases. Conclusions: In this study, we proposed a new tumor thrombus grading system based on the anatomical characteristics of retroperitoneal tumors with venous tumor thrombus. Retroperitoneal tumor resection and removal of venous tumor thrombus are safe and effective in the treatment of such diseases.
The Incidence and Risk Factors of Extrapulmonary Manifestations in Mycoplasma Pneumoniae Pneumonia
Authors: Park Yoo Kyung , Park You Na , Moon Ji Eun , Kim Hyo-Bin , Shin Mee Yong , Lee Eun , Kim Chul-Hong , Lee Ju Suk , Lee Yong Ju , Kim Bong-Seong , Kim Hyung Young , Jung Sungsu , Kim Yunsun , Kim Sangyoung , Park Chorong , Seo Ju-Hee , Shim Jung Yeon , Sol In Suk , Sung Myongsoon , Song Dae Jin , Ahn Young Min , Oh Hea Lin , Yu Jinho , Lee Kyung Suk , Jang Gwang Cheon , Jang Yoon-Young , Chung Hai Lee , Chung Eun Hee , Choi Sung-Min , Choi Yun Jung , Han Man Yong , Kim Jin Tack , Kim Chang-Keun , Yang Hyeon-Jong 
Published: December 2021

Abstract
Background: Mycoplasma pneumoniae pneumonia (MP) is a major cause of community acquired pneumonia (CAP) in children and it is known to be associated with extrapulmonary manifestations (EPM). The incidence and risk factors of EPM in children are not known.Methods: This is a retrospective study involving 65,243 pediatric CAP patients between 2010 and 2015 at 23 nationwide hospitals was conducted in South Korea. The medical records were reviewed to collect the information regarding the clinical characteristics, radiological results, and laboratory findings. In total, 9,190 children with MP were identified and included in the analysis. Logistic regression with multivariable analysis was performed to evaluate the risk factors associated with EPM in MP.Results: The mean age of the enrolled patients with MP was 64.3±39.8 months, and the proportion of male patients was 49.5%. The incidence of EPM was 23.9% and included elevation of liver enzymes (18.1%), mucocutaneous manifestations (4.4%), proteinuria (4.1%), cardiovascular and neurologic manifestation (0.4%), hematologic manifestation (0.2%) and arthritis (0.2%). Statistical analysis showed that mucocutaneous manifestations were significantly increased with elevated alanine aminotransferase (adjusted odds ratio [aOR] 3.623, 95% confidence intervals [CI] 1.933-6.790) and atopic sensitization (aOR 2.973, 95% CI 1.615-5.475) and decreased with respiratory virus co-infection (aOR 0.273, 95% CI 0.084-0.887). Elevated liver enzymes was significantly associated with the elevation of lactate dehydrogenase (aOR 3.055, 95% CI 2.257-4.137) and presence of pleural effusion (aOR 2.635, 95% CI 1.767-3.930) and proteinuria with respiratory virus co-infection (aOR 2.245, 95% CI 1.113-4.527). Conclusions: About 24% of pediatric MP patients were identified with various EPM. Since risk factors associated with each EPM was different, it is necessary to evaluate the various clinical aspects and findings of MP to predict and prepare for the occurrence of EPM.
Synthesis, Characterization and Application of Organoclay for Adsorptive Desulfurization: Isothermal, kinetic and Thermodynamics Studies
Authors: Saeed Muhammad , Riaz Aqsa , Intisar Azeem , Zafar Mazhar Iqbal , Fatima Humaria , Howari Haidar , Alhodaib Aiyeshah , Waseem Amir 
Published: December 2021

Abstract
The present study encompasses the application of cost effective, organo-modified bentonite material for efficient desulfurization of model oil and real fuel. For the adsorptive desulfurization of oil, dibenzothiophene (DBT) was used as model compound. Various experimental parameters (time, temperature, adsorbent-amount and DBT concentration) were thoroughly investigated. The synthesized material was characterized via X-ray diffraction (XRD), X-ray Fluorescence (XRF), Scanning electron microscopy (SEM), Energy dispersive x-ray (EDX), Thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR). The modification exhibits the increase in interlayer spacing of clay as confirmed from XRD and modified material shows interesting morphology as compared to unmodified bentonite. The results showed that >90% of DBT removal was achieved under optimized conditions for B-BTC, B-BTB and B-DSS and >80% for B-BEHA, for model fuel oil. Additionally, the findings from desulfurization of real fuel oil declare that 96.76% and 95.83% removal efficiency was achieved for kerosene and diesel oil respectively, at optimized conditions and fuel properties follow ASTM specifications. The obtained findings well fitted with thermodynamic, isothermal (Langmuir) with adsorption capacity (70.8 (B-BTC), 66 (B-BTB), 61.2 (B-DSS) and 55.2 (B-BEHA) in mg/g) and pseudo-second-order kinetics. In thermodynamic studies, negative sign (\(\varDelta G^\circ )\) specifies the spontaneity whereas, \(\left(\varDelta H^\circ \right)\) endothermic and positive sign \((\varDelta S^\circ )\) show randomness after DBT adsorption.
ANGPTL4 Negatively Regulate the Progression of Osteosarcoma by Remodeling Branched-Chain Amino Acid Metabolism
Authors: Lin Shanyi , Miao Yu , Zheng Xu , Dong Yang , Yang Qingcheng , Yang Quanjun , Xu Jun , Zhou Shumin , Yuan Ting 
Published: December 2021

Abstract
BackgroundAngiopoietin-like-4 (ANGPTL4), a secreted glycoprotein that is mainly recognized as a regulator in lipid metabolism, now, is implied in the regulation of the growth and metastasis of various carcinomas. However, less is known about its functions in the progression of sarcomas, let alone osteosarcoma (OS), which is the most common malignant diagnosed in musculoskeletal system.MethodsThe expression of ANGPTL4 in clinical OS samples and cell lines paired with their controls were analyzed in both mRNA and protein levels. Cell functional analysis including proliferation and colony formation were carried out to detect the roles ANGPTL4 takes in the progress of OS using stable ANGPTL4 overexpression and knockdown HOS/MNNG cell lines. The RNA-Seq and bioinformatics analysis were then employed to discover the BCAA metabolism related signaling which is involved in ANGPTL4 functioning on HOS/MNNG cell growth. Furthermore, BCAAs content measurement, and BCATs rescue experiments were performed to confirm the BCAA/mTOR signaling axis that ANGPTL4 triggered in HOS/MNNG cells. Finally, a xenograft mouse model was carried out to further verify the ANGPTL4 /BCAA/mTOR signaling axis discovered. ResultsWe found that the expression of ANGPTL4 is reduced in clinical OS tissues and cell lines compared to cancellous bone tissues and BMSCs, respectively. The knockdown of ANGPTL4 in HOS/MNNG cells results in enhanced cell growth and clone formation. Moreover, BCAA/mTOR signaling axis were discovered to be triggered by ANGPTL4 down regulation in HOS/MNNG cell using RNA-seq. It was also verified that the accumulation of BCAAs activates the mTOR signaling pathway, and in turn promotes HOS/MNNG cell growth using BCAAs content measurement, and BCAT inhibition. Finally, the IHC results of xenograft mouse model also confirmed this ANGPTL4/BCAA/mTOR signaling axis in vivo.ConclusionsTaken together, our results demonstrate that the expression of ANGPTL4 were negatively related to OS progress. Moreover, it was found the down-regulation of ANGPTL4 promoted OS cell growth via BCAAs/mTOR axis.
WITHDRAWN: Mapping evidence on strengthened and sustained delivery early detection and management of Hypertension and Diabetes among PLWH: a scoping review protocol
Authors: Badacho Abebe sorsa , Mahomed Ozayr H 
Published: December 2021

Abstract
ObjectiveThe main review objective of this scoping review is to identify the evidence on the patient outcomes of integrated NCD and HIV services, the type of models used, barriers and facilitators of effective integrated services for early detection and management of hypertension and diabetes in adults PLWH in primary health care settings of Low-income countries. Introduction:Non-communicable diseases (NCDs) raise global public health challenges in all population groups, but the prevalence of hypertension and diabetes is highly rising among people living with HIV (PLWH). This population group requires evidence-based care for early detection and management of hypertension and diabetes. However, there is a lack of evidence regarding the impact of integrated NCD and HIV services on outcomes of PLWH, its feasibility and effectiveness of services provision and the type of models used in strengthened and sustainable services for early detection and management of hypertension and diabetes in primary health care settings. It is necessary to map and models of integrated NCD and HIV services for PLWH and its feasibility and effectiveness as well patient outcomes in a primary health care setting in low-income countries Inclusion criteria: This scoping review will consider studies focusing on the model of integrated Non-communicable and HIV services for adult people living with HIV, including the type of models used, its feasibility, effectiveness and outcome of integrated services in primary health care settings. All published and grey literature will be included in the review. Methods: The search will be carried out using PubMed, MEDLINE with full text via EBSCO host, Google Scholar, Science Direct and Scopus, for a comprehensive search. Using MESH terms, an advanced search will be conducted and also will include grey literature through OpenGrey. This review will be restricted to studies published in English because of the feasibility of translating languages other than English. A three-step search strategy will be used, and the articles identified in the databases will be organised, and the duplicates will be removed. Two independent reviewers will review the titles and abstracts. Full texts will be imported into a bibliographic reference management system. The findings will be presented in tables and descriptive summaries.
Myeloid leukemia vulnerabilities at CTCF-enriched long noncoding RNA loci
Authors: Ng Michelle , Verboon Lonneke , Issa Hasan , Bhayadia Raj , Alejo-Valle Oriol , Borchert Dorit , Schuschel Konstantin , Regenyi Eniko , Reinhardt Dirk , Yaspo Marie-Laure , Heckl Dirk , Klusmann Jan-Henning 
Published: December 2021

Abstract
The noncoding genome presents a largely untapped source of biological insights, including thousands of long noncoding RNA (lncRNA) loci. While some produce bona fide lncRNAs, others exert transcript-independent cis-regulatory effects, and a lack of predictive features renders mechanistic dissection challenging. Here, we describe MYNRL15, a CTCF-enriched lncRNA locus and pan-myeloid leukemia dependency initially identified by expression-guided CRISPR interference screens. We show that accessibility and integrity of the MYNRL15 locus is required for myeloid leukemia maintenance; its perturbation selectively impairs acute myeloid leukemia (AML) cells compared to hematopoietic stem and progenitor cells in vitro, and depletes AML xenografts in vivo. While the MYNRL15 transcript and neighboring protein-coding genes appear dispensable, dense CRISPR tiling of the locus revealed two crucial candidate cis-regulatory DNA elements which drive the perturbation phenotype. Disruption of these elements triggers the formation of a tumor-suppressive, long-range chromatin interaction. By integrating transcriptome profiling with a CRISPR-Cas9 knockout screen of genes from the gained interaction region, we pinpointed two downregulated, potent cancer dependency genes as effectors of MYNRL15 disruption: WDR61 and IMP3. Finally, guided by distinctive features of the MYNRL15 locus, we find that elevated CTCF density characterizes a set of lncRNA loci enriched in leukemia vulnerabilities (22.6-24.2% essentiality rate). A catalog of CTCF-enriched lncRNA loci (C-LNCs) in 18 cell types representing different cancer entities and tissues is provided with this study, towards refining the search for noncoding oncogenic vulnerabilities in leukemia and other malignancies.
Current carbon storage in forests of two ecoregions of Russia
Authors: Усольцев В.А. , Ковязин В.Ф. , Цепордей И.С. 
Published: December 2021

В связи с глобальным потеплением климата оценка углеродного цикла в лесных экосистемах приобрела особое значение. Один из методов определения депонированного в лесах углерода основан на использовании конверсионных коэффициентов биомассы (ККБ) и данных Государственного учета лесного фонда (ГУЛФ). Путем объединения моделей ККБ с данными ГУЛФ в двух экорегионах России – таежном и лесостепном – было установлено, что за 20–25- летний период накопление органического углерода в таежной зоне значительно меньше (5%) по сравнению с лесостепной зоной (39%). Несмотря на существующие риски стихийных бедствий в лесостепном экотоне, за четверть века наблюдается значительный рост депонированного углерода. Это произошло вследствие высокой доли молодняков в начале анализируемого периода, обладающих повышенным приростом по отношению к спелым древостоям. Сопоставимые результаты были получены одним и тем же методом в разных экорегионах планеты: от 8% за 5 лет в Китае до 68% за 50 лет в Японии. Сравнение результатов, полученных предложенным методом и методом IIASA (Австрия), показало минимальное расхождение (3%), что дает основание считать полученные оценки депонирования углерода близкими к реальности. Однако сохраняется неопределенность, связанная с качеством данных ГУЛФ и депонированием углерода в почве.
Due to the global warming of the climate, the assessment of the carbon cycle in forest ecosystems has become particularly important. One method for determining deposited carbon is based on the use of biomass expansion factors (BEF) and State Forest Inventory (SFI) data. By combining BEF models with SFI data in two ecoregions of Russia – taiga and forest-steppe – it was found that over a 20–25-year period, accumulating the carbon deposition in the taiga zone is significantly less (5%) compared to the forest-steppe zone (39%). Despite the existing risks of natural disasters in the forest-steppe ecotone, there is a significant increase in carbon deposition over a quarter of a century. This was due to the high proportion of young stands at the beginning of the analyzed period, which have increased growth in relation to old stands. Comparable results were obtained by the same method in different ecoregions of the planet (from 8% in 5 years in China to 68% in 50 years in Japan). A comparison of the results obtained by the proposed method and the IIASA (Austria) method showed a minimal discrepancy (3%), which gives reason to consider the above estimates of carbon deposition close to reality. However, uncertainties remain related to the quality of the SFI data and the carbon deposition in the soil.
Analysis of the polymorphism of microsatellite loci in Picea abies (L.) H. Karst. and Picea obovatа Ledeb populations
Authors: Волков В.А. , Калько Г.В. 
Published: December 2021

Процессы генезиса хвойных лесов интенсивно изучаются с использованием методов молекулярного маркирования. Микросателлитные локусы рассматриваются в качестве наиболее полиморфной и воспроизводимой системы кодоминантных молекулярных маркеров. В цели исследования входило установить возможность использования микросателлитных маркеров для определения видовых различий между елью европейской и сибирской (Picea abies и Picea obovatа), а также оценить степень генетической обособленности региональных популяций ели на территории Европейской части РФ. Исследование показало, что использование набора из двенадцати микросателлитных маркеров позволяет разделить выборку елей, собранных с европейской и азиатской частей страны, на два генетических кластера, соответствующих видам Picea abies и Picea obovatа. Проанализированные в исследовании микросателлитные локусы могут быть использованы для оценки генетического разнообразия и географического происхождения ели европейской и сибирской.
The processes of genesis of coniferous forests are widely investigating using molecular markers. Many studies are based on the analysis of microsatellite loci, which are acknowledged as the most reproducible and polymorphic co-dominant molecular markers. This study is aimed to establish the possibility of using microsatellite markers to determine differentiation between Norway and Siberian spruce (Picea abies and Picea obovata). Another task of this research was to assess the degree of genetic isolation of regional spruce population in the territory of European part of the Russian Federation. This study shows that using a large number of microsatellite markers allows to divide the sample of spruce collected from European part of Russia and Siberia in two genetic clusters, corresponding to Picea abies and Picea obovata. The microsatellite loci analyzed in the study can be used to assess genetic diversity and geographic origin of spruce trees and determine the origin of wood and planting material of Norway and Siberian spruce.
CESTA BÁSICA DE CAICÓ (RN)
Authors: Freitas Fontes Júnior Deylane , Duarte de Araújo Iarythssa , Teixeira Ana Paula , Miranda Ferreira Almir 
Published: December 2021

Com base legal e nas análises realizadas pelo DIEESE, o grupo PET Comunidade Urbana Seridó (PET COURSE) desenvolve um projeto de extensão para calcular o custo mensal de 12 itens que compõem a Cesta Básica no município de Caicó (RN). Assim, o objetivo geral deste relatório é: analisar a variação do custo médio da cesta básica de Caicó no ano de 2020. Metodologicamente, os dados são coletados presencialmente em cinco supermercados mensalmente e disponibilizados em planilhas. Também foi desenvolvido um estudo documental, sobretudo, com análise da pesquisa nacional desenvolvida pelo DIEESE. Os principais resultados encontrados mostram: um aumento de 20,6% do custo médio da cesta básica em Caicó, no ano de 2020, além de índices significativos, como o custo familiar alimentar de R$ 1.242,63 em dezembro.