bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of Antioxidant Response With Compatible Combination of Mesorhizobium SP. and Pseudomonas SP. Against Fusarium Oxysporum SP. Ciceris in Chickpea
Authors: Nagpal Sharon , Sharma Poonam , Sirari Asmita 
Published: June 2021

Chickpea (Cicer arietinum L.) is severely affected by Fusarium oxysporum sp. ciceris causing vascular wilt disease accounting about 10% of yield losses. The present study was conducted to assess the potential of compatible dual inoculants of Mesorhizobium sp. and PGPR (rhizospheric and endophytic) in initiating and boosting the antioxidant response towards the phytopathogen in vivo under wilt sick conditions in chickpea. Among the different dual inoculated treatments, LGR191+NE8 (Mesorhizobium sp. + nodule endophyte) showed maximum augmentation in the antioxidants viz., catalase (15.16 U/min/g fresh weight of root), guaiacol peroxidase (2.492mmoles/min/g fresh weight of root) and total phenolics (110.25 tannic acid equivalents/g fresh weight of root) over recommended fungicide (captan). Percent wilt control was highest with LGR191+NE8 (95.77%) followed by LGR1+LPGPR-1 (94.17%) after 120 days of sowing. The results suggested an expanded antioxidant state of the wilt infected plants with the dual inoculation of Mesorhizobium sp. with PGPR (rhizospheric and endophytic) decreasing fungal proliferation sustainably.
Bangladesh J. Bot. 50(2): 359-364, 2021 (June)
Genetic Diversity Within and Among Populations of Shorea Robusta Roxb. Ex Gaertn. In Bangladesh and Its Implications for Conservation
Authors: Sharmi Pragga Saha , Kashem Md Abul , Samad Rifat , Hossain Mohammad Zabed 
Published: June 2021

Fragmentation and reduction of natural population size render threats to the conservation of forest resources through depletion of genetic diversity. Hence, information on genetic structure of Sal (Shorea robusta Roxb. ex Gaertn.) populations is relevant for proper management and conservation of the tropical deciduous forests. The present study focused on assessing the genetic diversity of the populations of Sal which was the dominant tree species of the deciduous forests of Bangladesh. Plant leaf samples were collected from the three populations of Sal distributed in the three geographical regions including Madhupur tract in the districts Tangail and Gazipur and that of the districts of Cumilla and Dinajpur. DNA band profiles were generated using eight ISSR primers for a total of 13 samples taken from the three populations. Statistical analysis was done using PopGen 32 and GenAlEx 6.5 softwares. Principal coordinate analysis done on the DNA band profiles revealed that Sal populations of Madhupur tract and Cumilla positioned nearby while Dinajpur showed maximum genetic distance with that of Cumilla. Mantel test showed significant (p=0.05) correlation between genetic and geographic distances indicating “Isolation by Distance”. Data of the present study indicated higher genetic polymorphism (68.87%) in the Sal population of Madhupur tract compared to other two populations. Small population size of Sal of Dinajpur forest might be related with its low genetic diversity. Data of the present study suggest immediate attention for the conservation of Sal forests in Bangladesh before further genetic erosion occurs.
Bangladesh J. Bot. 50(2): 405-412, 2021 (June)
Analysis of Chronic Disease Direct Cost at Public Primary Health Cares in Indonesia
Authors: Sulistyaningrum Indriyati Hadi , Kristina Susi Ari , Mukti Ali Ghufron , Satibi - 
Published: June 2021

Objectives: This study aims to analyze the of drug costs for patients with chronic diseases in the capitation system in public primary health cares (Public PHCs).
Materials and Methods: This research is a quantitative study with an observational cross-sectional approach at 16 Public PHCs. Data collection used the retrospective method. The total sample was 293 outpatients in 2016.
Results: The results showed that 63% of patients are female. The most patients are aged between 56-65 years (37%). The average drug cost for patients receiving more than 3 types of drugs is IDR 7,726. The biggest drug cost is patients with DM type 2 with which the average cost is IDR 7.400. And the metformin treatment is 17 (6%) patients with the cost IDR 4,500,-. Chi-square analysis shows that the quantity of drug items, type of disease and prescriptions there are have significant effect on drug cost (p-value <0.05). However, gender and age group there are no significant effect on drug cost (p-value >0.05). Drug prescriptions are an important component in managing chronic diseases patients. Prescribing costs provide important information in the sustainability of the management of chronic disease programs.
Conclusion: This study recommends that the prolanis policy in public PHCs is focused on the elderly age group patients and female group.
Bangladesh Journal of Medical Science Vol.20(4) 2021 p.762-767
Mini-invasive revascularization operations in the complex treatment of neuroischemic and ischemic forms of diabetic foot syndrome
Authors: Zhakiyev Bazylbek , Jakanov Murat , Zhanabaev Bernat , Koyshibaev Anuar , Mukanov Samat , Aitbayeva Aliya 
Published: June 2021

Objective: Diabetic foot syndrome is the most dramatic complication of diabetes mellitus. The treatment success is impossible without improving blood circulation and the elimination of hemodynamic disorders in the affected limb.The study aim was to evaluate mini-invasive revascularization operations effectiveness on the lower limbs peripheral arteries in neuro-ischemic and ischemic diabetic foot syndrome forms.
Materials and methods: The study enrolled 104 patients with purulent-necrotic complications of diabetic foot syndrome. 54 (51.9%) patients underwent stenting and balloon angioplasty (the main group). The comparison group included 50 (48.1%) patients, undergoing indirect revascularization surgeries in complex treatment.
Results and discussion:After endovascular interventions, the API index increased by 47.6% (p<0.01) after 20 days compared to the admission index, the average blood flow rate increased by 1.8 times (p<0.01), and the tPo2 value improved by 67.8% (p<0.01). In the control group, the tPo2 value increased by 40.3% after 20 days, and API – by 34.4%. In the main group, the swelling disappearance in the foot wounds area was by 3.7±0.5 days faster (p<0.001), wounds purification by 4.1 days (p<0.001), granulation appearance by 3.5±0.4 days (p<0.001), wound margins infiltration disappearance happened 3.3±0.9 days earlier (p<0.001), and the wound epithelization was 2.9 days earlier (p<0.01) than in the control group patients.
Conclusion: Foot support function at discharge from hospital was preserved in 94.4% of the main group and in 84% of the control group.
Bangladesh Journal of Medical Science Vol.20(4) 2021 p.817-825
Study of genetic variability of umbilical cord blood using RAPD assay
Authors: Miah Md Faruque , Rahaman Md Shad Ebna , Chowdhury Sanjana Fatema , Mahmud Mohammad Golam Rob 
Published: June 2021

Background: The genetic variability of Umbilical Cord Blood (UCB) is most important for newborn screening, therapeutic possibility of haematological disorders as well as for the establishment of cord blood banking and stem cell research.
Method: Genetic variability of umbilical cord blood (UCB) of 22 human subjects was evaluated first time by applying Random Amplified Polymorphic DNA (RAPD) assay using six decamar primers (B-14, OPB-05, OPB-08, OPB-12, OPB-19 and UBC-122).
Result: A total number of bands were recorded 312 from 116 polymorphic loci and single monomorphic locus. All the markers showed highest polymorphism (100%) except the primer OPB 08 (92.31%) among tested individuals. The genetic distance was observed with highest 1.0 and lowest 0.72 respectively whereas mean genetic distance was recorded 0.90. Considering Shannon-Wiener index average diversity was recorded 0.139365. The mean Nei genetic similarity was found 0.17 which was found opposition to genetic distances. A phylogenetic relationship among the individual subjects was also observed between the linkage distances of 11 to 27 with 8 clades, 3 subclusters and a cluster. In addition, average allele frequency p and q was observed 0.08156 and 0.948751 respectively whereas highest intra locus gene diversity and average gene diversity were found 3.323817 and 0.144572 respectively.
Conclusion: Considering different parameters, higher genetic variability was found among the experimental subjects, probably due to the mixture DNA of parents and newborn.
Bangladesh Journal of Medical Science Vol.20(4) 2021 p.848-854
Obesity in relation to clinical, endocrine and metabolic parameters in infertile women with polycystic ovary syndrome: the South Asian perspective
Authors: Ishrat Shakeela , Hossain Marufa 
Published: June 2021

Background: Polycystic ovary syndrome (PCOS) affects 5-10% of reproductive age women and it is a common cause of infertility in young women. Most of the infertile women with PCOS are overweight or obese. Obesity or excess fat aggravates the endocrine and metabolic dysfunction in women with PCOS. Weight management is the first line measure advised to infertile PCOS women who are overweight or obese. The objective of the study was to explore the association of obesity with the clinical, endocrine and metabolic parameters in infertile women with polycystic ovary syndrome in Bangladesh.
Methodology: This was a cross sectional study of 126 consecutive infertile women with polycystic ovary syndrome attending the Infertility unit of the Department of Obstetrics and Gynecology at Bangabandhu Sheikh Mujib Medical University from January 2017 to December 2017.Obesity groups were defined by BMI thresholds specific for the South Asian population.
Results: The mean body mass index (BMI) was 26.58±3.18 kg/m2and mean waist circumference was 91.07±9.5 cm. There was highly significant association of obesity with waist circumference and fasting insulin. BMI at or above 25kg/m2 was significantly associated with acanthosis nigricans, hyperandrogenemia and hyperinsulinemia, whereas BMI at or more than 23kg/m2 was significantly associated, in addition, with insulin resistance and metabolic syndrome.
Conclusion: Obesity is associated with hyperandrogenemia, hyperinsulinemia, insulin resistance and metabolic syndrome in infertile women with polycystic ovary syndrome.
Bangladesh Journal of Medical Science Vol.20(4) 2021 p.864-870
Positional Hand Pallor and Isolated Epitrochlear Lymphadenitis Secondary to Bartonella henselae Infection.
Authors: Jamil Siti Nuradliah , Ismail Ilham Ameera , Sham Siti Fatimah Badlishah , Mohamad Ali Norliana Dalila 
Published: June 2021

Cat scratch disease is a communicable disease caused by the Bartonella henselae bacteria. Regional lymphadenopathy is the hallmark of cat scratch disease and about 75% of lymphadenopathy cases are localized in the head and neck region. An epitrochlear lymphadenopathy is a rare condition at any age and often misdiagnosed as it is not normally palpable. External compression of an enlarged epitrochlear lymph node compromising vascularity was not mentioned in any literature before. We present a case of a 13-year-old girl with right positional ipsilateral hand pallor and epitrochlear lymphadenitis with serological evidence of Bartonella henselae infection.
Bangladesh Journal of Medical Science Vol.20(4) 2021 p.914-917
Carbetocin versus Oxytocin in Active Management of 3rd stage of Labour following Vaginal Delivery
Authors: Ashraf F , Akther Pervin , Yasmin N , Islam JA , Akther M , Rahman R , Ahmad SA , Faruquee MH , Islam GMR , Rakib MHA 
Published: June 2021

Background: Every day more than 220 women around the world die from severe bleeding after childbirth. Globally post-partum hemorrhage is the number one direct cause of maternal mortality. Most postpartum hemorrhages are caused by uterine atony and occur in the immediate postpartum period. Most of these tragic deaths can be prevented by active management of third stage of labour. Active management of the third stage of labor should be practiced routinely to decrease the risk of postpartum hemorrhage. Oxytocin is used for enhancing uterine contraction after delivery. But oxytocin has some limitations like shorter half- life, less contraction time and more side effects, whereas carbetocin has prolonged duration of action which ensures more contraction time and less adverse effects. This study was done to see the efficacy and safety of carbetocin over oxytocin for prevention of PPH after vaginal delivery.
Methodology: A randomized controlled clinical trial was conducted in the Department of Obstetrics and Gynecology, Shaheed Suhrawardy Medical College and Hospital, Dhaka, Bangladesh over a period of 9 months from January 2015 to September 2015. Ninety four patients undergoing vaginal delivery at term were randomized into two groups receiving either 10IU oxytocin or 100 μg carbetocin. Outcome measures such as primary PPH, massive blood loss, need for additional uterotonic drug, additional blood transfusion as well as adverse effects were documented.
Results: In this study, massive blood loss did not occur none of patients in carbetocin group. But massive blood loss occured 6.4% women of oxytocin group. Further fundal massage , immediate blood transfusion and additional uterotonics didn’t need any patient in carbetocin group. In oxytocin group, fundal massage required in 8.5% of women, blood transfusion needed in 10.6% patients and additional uterotonics needed in 10.6% women. Average amount of blood loss was 88 ml less in carbetocin group and adverse effects of drugs were almost similar in both group. Primary PPH developed in oxytocin group 8.5% but none of patients had developed PPH in carbetocin group.
Conclusion: Carbetocin is an effective new drug than oxytocin for prevention of postpartum hemorrhage in vaginal delivery.
Bioresearch Commu. 7(1): 927-931, 2021 (January)
Mycoflora associated with diseased rice grains in Bangladesh and their pathogenic potentiality
Authors: Chowdhury Pranami , Shamsi Shamim , Begum Hasna Hena , Bashar Md Abul 
Published: June 2021

Fifty six spotted rice grain samples of four commercially cultivated rice varieties namely BRRI 28, 29, Kalijira and Pajam were collected from 14 different districts of Bangladesh. Forty rice samples (Hybrid 2,3,4, BR7,11,12,14,16,22,23,25,26and BRRI28 to BRRI 55) were also collected from Bangladesh Rice Research Institute at Joydevpur. Twenty-fivespecies of fungi belonging to 15 genera were associated with these rice varieties. The isolated fungi were Alternaria alternata, Aspergillus clavatus, A. flavus, A. fumigatus, A.niger, A. ochraceus, A.oryzae, A. terreus, Chaetomium globosum, Cladosporium cladosporioides, Colletotrichum gloeosporioides, Curvularia lunata, C. lunata var. aeria, Drechslera oryzae, Fusarium moniliforme, F. oxysporum, F. solani, Microdochium oryzae, Nigrospora oryzae, Penicillium spp., Pestalotiopsis guepinii, Sarocladium oryzae and Trichoderma viride. Amongst these fungi nine i.e. Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link, Curvularia lunata (Wakker) Boedijn, Drechsler aoryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon, F. solani (Mart.) Sacc., Microdochium oryzae (Hashloka and Yokogi) Sam. and Hal., Pestalotiopsis guepinii (Desm.) Stay. and Sarocladium oryzae (Sawada) W. Gams and D. Hawks were found to be pathogenic to rice seeds. The most predominant fungus was D. oryzae which was followed by A. flavus and the least incidence was observed in case of F. solani and P. guepinni.
Bioresearch Commu. 7(1): 932-940, 2021 (January)
Design of Smart Nurse Call (Caller Nurse) Based on Android
Authors: Septian Pradita Ghanda 
Published: June 2021

The limited number of nurses is a problem that often occurs in the world of health which has an impact on the high workload of nurses resulting in less optimal patient care and decreased patient satisfaction. According to Institute for Healthcare Improvement, nurses spend only 20-30% of their time in direct patient care, the rest on documentation and administration processes. So, hospitals generally provide nurse calls so families don't have to leave patients to find nurses.The purpose of this study was to make it easier for patients to use Smart Nurse Call, especially for patients with acute diagnosis conditions and it's easier for nurses to monitor patient's condition. It's also examines the distance of data transmission in one building to determine the performance of wireless (access points).Based on data from test results, the quality of data transmission using wireless media is known that access point has data transmission limit. In testing distance at AH building stated that data transmission based on the range on the 1st floor is able to reach the entire corridor, for 2nd floor it can reach a distance of 13 meters while on 3rd floor it cannot be reached. The data from suitability test of smart nurse call tool showed that the suitability level of the device was quite high and almost same as manual test at 97.65%. The data from stress test results from push button get average voltage value low position at 0 V and average high voltage at 4.675 V.
Smart Cat Home With Control System using Telegram Application
Authors: Putri Alfi Safira Anandatito 
Published: June 2021

Survey data by the World Society for the Protection of an Animal(WSPA) in 2007 recorded that total pet population in Indonesia was 23,000,000 individuals (Batson,2008). The majority of Indonesian choose to keep purebred cats. Care of this breed is'nt easy and requires special attention from the owner. With Smart Cat Home, cat owners can monitor and control feeding schedule, temperature, and drinking conditions in cat cage via their telegraphic social media. The Smart Cat Home is designed using Raspberry Pi3 microcontroller. This system functions to monitor feeding schedule according to cat habits, determine weight of cat feed detected by Loadcell, monitor availability of drinking water by water level sensor, capture cat's condition by webcam, and temperature monitoring using DHT11 sensor. All conditions on system will be displayed on Telegram Bot. From test results can be concluded that the whole system works well. In temperature system, the warmer will be active when <22°C, and the fan will be active at temperature >26°C. In feeding system, the weight sensor is able to read feed weight accurately. From 5 data on feed weight, the average feed weight is 78 grams. In drinking system, the drinking water reservoir will fill drinking container when water level sensor have voltage value <1600 mv with water level of 2.5cm from the surface. From the calculation of delay value in sending data, there's delay of 33.8ms. Packet loss or the amount of data lost during transmission process is 0.3%. Througput of data transmission in system is 84,139,041 Kbytes/sec.
Cardiac Magnetic Resonance Predicting Outcomes Among Patients at Risk for Cardiac AL Amyloidosis
Authors: Agha Ali M. , Palaskas Nicolas , Patel Amit R. , DeCara Jeanne , Parwani Purvi , Iliescu Cezar , Durand Jean B. , Kim Peter , Hassan Saamir , Gladish Gregory , Lee Hans C. , Kaufman Gregory P. , Lopez-Mattei Juan C. 
Published: June 2021

Introduction: Patients with systemic AL amyloidosis (AL) should be evaluated for cardiac amyloidosis (CA), as prognosis is strongly related to cardiac involvement. We assessed the characteristics of patients referred to cardiac magnetic resonance (CMR) with suspected CA from a cancer center and determine predictors of mortality/heart failure hospitalizations (HFH).Methods: Forty-four consecutive patients referred for CMR with suspected CA were retrospectively included. Variables collected included cardiac biomarkers, in addition to echocardiographic and CMR variables. Survival analyses were performed to determine which variables were more predictive of mortality and HFH.Results: Of the 44 patients included, 55% were females. 73% of patients were diagnosed with CA by CMR; 56% of them had an established diagnosis of AL. Patients with CA by CMR had higher native T1, higher extracellular volume (ECV) fraction, higher T2, less negative GLS by Echo, and higher troponin I and B-type natriuretic peptide (BNP). Kaplan-Meier survival analysis revealed that the following were predictive of mortality: an ECV ≥ 0.50 (p = 0.0098), CMR LVEF &lt; 50% (p = 0.0010), T2/ECV ≤ 100 (p = 0.0001), and troponin I &gt; 0.03 (p = 0.0025). In a stepwise conditional Cox logistic regression model, the only variable predictive of a composite of mortality and HFH was ECV (HR: 1.17, 95% CI = 1.02–1.34 p = 0.030).Conclusion: ECV seems to be an important biomarker that could be a predictor of outcomes in cardiac AL amyloidosis. In combination, CMR and serum cardiac biomarkers might help to establish prognosis in patients with CA.
Common Variants in NUS1 and GP2 Genes Contributed to the Risk of Gestational Diabetes Mellitus
Authors: Zhang Tianxiao , Zhao Longrui , Wang Shujin , Liu Juan , Chang Ying , Ma Louyan , Feng Jia , Niu Yu 
Published: June 2021

BackgroundRecently, NUS1 and GP2 genes were reported to be associated with the risk of type 2 diabetes (T2D) in a Japanese population. Given the sharing of pathogenic contribution from genetic factors between T2D and gestational diabetes mellitus (GDM), we conducted the study to systematically examine the relationship of NUS1 and GP2 genes with the susceptibility to GDM in Chinese Han population.MethodsA total of 4,250 subjects comprised of 1,282 patients with GDM and 2,968 controls were recruited, and 20 tag single nucleotide polymorphisms (SNPs) (10 from NUS1 and 10 from GP2) were selected for genotyping. Association analyses were conducted for GDM and its related biomedical indexes including fasting glucose and HbA1c levels.ResultsTwo SNPs, rs80196932 from NUS1 (P=2.93×10-5) and rs117267808 from GP2 (P=5.68×10-5), were identified to be significantly associated with the risk of GDM. Additionally, SNP rs80196932 was significantly associated with HbA1c level in both patients with GDM (P=0.0009) and controls (P=0.0003), while SNP rs117267808 was significantly associated with fasting glucose level in both patients with GDM (P=0.0008) and controls (P=0.0007). Serum levels of protein NUS1 and GP2 were measured for the study subjects, and significant differences were identified among groups with different genotypes of SNP rs80196932 and rs117267808, respectively.ConclusionsOur findings indicate that NUS1 and GP2 genes contribute to the risk of GDM, which would help to offer the potential to improve our understanding of the etiology of GDM and, in turn, could facilitate the development of novel medicines and treatments for GDM.