bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improvements in monofloral honey quality control
Authors: Serebryakova O V , Rusakova T M 
Published: January 2021

Abstract
The paper presents the results of determining the activity of the most important honey enzyme, invertase, depending on the impact of main technological factors, as well as identifying the minimum permissible enzyme content in raw and processed honey. The studies were carried out in the laboratory of the Federal Beekeeping Research Center. The invertase activity and diastatic number was determined by a photometric method. The honey samples were examined in five replicates. The enzyme activity was determined after 30 and 90 days of honey storage. Deep freezing of honey at a temperature of -18 °C ensured the best preservation of the invertase activity in monofloral honey that came from an assortment of plants. Pasteurization of natural honey reduced the activity of enzymes of honey that comes from different plants by an average of 71.9%. Mechanical treatment of honey reduced the invertase activity by an average of 54.7%. Based on a comparative analysis of invertase and diastatic number, invertase was found to reflect the degree of honey treatment in the best way. The research findings will result in a set of recommendations to enhance the technology for honey processing and quality control, with a subsequent introduction of this indicator in the state standard for natural honey.
Effect of presowing seed treatment on photosynthetic pigments content and enzyme activity in Hordeumvulgare L. plants
Authors: Voskobulova N I , Neverov A A , Vasilieva T N , Schukin V B , Terekhova N A 
Published: January 2021

Abstract
The use of nanoparticles in pre-sowing seed treatment increases the resistance of plants to pathogenic microflora, adverse weather conditions and increases the yield of crops. The study aimed to study the effect of molybdenum nanoparticles, a suspension of iron and silicon and preparations based on humic acids on the content of photosynthetic pigments, the activity of antioxidant enzymes in Hordeumvulgare L. plants and its productivity. The content of chlorophyll a and b, carotenoids in different phases of plant development was determined using the standard method with Smashevsky modifications, catalytic activity according to the Ebi method, superoxide dismutase according to Hyannopolitis and Rice with some modifications of Polesskaya. Barley seed treatment with preparations based on iron, silicon, molybumum, bogum and molybdenum nanoparticles M Mo stimulates an increase in the level of photosynthetic pigments and the activity of antioxidant enzymes in plants. Using a composition created based on Fe3O4 (10–3 mg/L) and SiO2 (10–4 mg/L) nanoparticles in a 1: 1 ratio for the presowing treatment of barley seeds increases grain yield.
Impact of the use of essential trace elements (zinc, copper) on the chemical composition of perennial medicinal plants in southern Western Siberia
Authors: Zharkova N N , Suhotskaya V V , Ermohin Yu I 
Published: January 2021

Abstract
The studies were conduct in 2012-2018 on meadow chernozem soil in the conditions of the south of Western Siberia. The purpose of the study was to study the effect of zinc and copper fertilizers on the chemical composition of perennial medicinal plants in the zonal soil-climatic conditions of Western Siberia. The objects of study were Achillea millefolium L., Tanacetum vulgare L. and Echinacea purpurea L. Zinc and copper fertilizers were applied in MAC fractions: 0.25, 0.50, 0.75, and 1.0 on the grounds N135P45K45 (A. millefolium, T. vulgare) and N125 (E.
purpurea). The use of zinc and copper fertilizers in the main application for the studied medicinal crops contributed to an increase in the accumulation of mobile forms of zinc and copper in the medicinal raw materials. On average, over the years of research in experiments with A. millefolium, T. vulgare, E.
purpurea, each kg of Zn fertilizers increased the Zn content in plants by 0.30 and 0.38, Cu - by 0.07, 0.05 and 0.03 mg / kg; while 1 kg of fertilizer added by Cu increased the Cu content by 0.47, 0.07 and 0.23, Zn by 1.40, 2.32 and 1.26 mg / kg, respectively. A synergistic relationship has been establish between the elements of Zn↔Cu and Cu↔Zn.
Environmental aspects of the use of ultrafine particles SiO2 (as exemplified by Solanum tuberosum)
Authors: Aminova E V , Mushinskiy A A , Korotkova A M 
Published: January 2021

Abstract
The interest in the use of ultrafine particles in crop production and agricultural practice is associated with the unique properties of ultrafine particles (UFP). At the same time, it is obvious that artificial nanoobjects can have toxic properties. Therefore, for further development of nanotechnologies, a clearer understanding of both the properties of nanomaterials themselves and the mechanisms of their interaction is needed. The article presents studies on the effect of ultrafine particles of silicon dioxide on plants Solánum tuberósum. According to the results of research nano-silica at a concentration of 0.21 and 0.36 g/kg had a statistically significant effect on the number of cell integrity (a decrease to 17.1 % was observed). It was revealed that after processing potato tubers with UFP silicon oxide at a concentrations of 0.09 and 0.18 g/kg, they had a significant influence on the activity of peroxidase. The results of the studies revealed the toxicity of UFP SiO2 at a maximum dilution of 0.36 g / kg, which confirmed a decrease in the intensity of the luminosity peaks of DNA a temporally calculated linear profile. Therefore, assessing the impact of SiO2 use is of great importance for understanding the long-term environmental impact of UFP.
Practical aspects of the new approach to creating feed products based on a multicomponent mixture of sunflower fuzz-sludge and zeolite subjected to cavitation treatment
Authors: Bykov A V 
Published: January 2021

Abstract
The problem of waste management in Russia is one of the fundamental. Moreover, in addition to European standard, according to which our society tends to consider the fact that an effective economy can only exist on the basis of non-waste production. The level of social development for a variety of symptoms can largely be characterized by the effective use of agricultural waste. Waste oil industry are characterized by a high content of protein and fat, which makes them indispensable in balancing rations. In this context shows how to obtain feed product based on waste vegetable oil extraction industry, enriched zeolite powder. The method is based on joint ultrasound treatment of fatty foods (sunflower Pusan), and mineral – zeolite. The efficiency of the product in poultry feeding.
Measurement of soil moisture by means of resonance control
Authors: Lisichkin V G , Ashikhina L A , Bolshakova L S , Izvekova E V , Kuzina A V , Ladnova O L , Lisichkina N V , Merkulova E G 
Published: January 2021

Abstract
The article is devoted to the research of innovations in the field of improvement of resonance methods of soil moisture control with the help of capacitive sensors. Soil moisture is one of the most important indicators of the hydrological state of the land, the variability of which is determined by both the soil structure and the impact of atmospheric processes. Study of the state and spatial and temporal dynamics of soil moisture is necessary not only for solving local applied problems of agrotechnical character, but also for conducting fundamental research in the field of ecology, hydrology, meteorology, climatology, including the forecasting of global climate change. In order to increase the accuracy of resonance control devices, it is proposed to use multiparameter methods of measurement and linear frequency modulation of the signal that excites the measuring transducer. Analytical studies of the processes associated with the appearance of additional measurement errors arising from the influence of active losses in the controlled soil and the “drift” of resonance frequency have been carried out, and ways of reducing these errors have been identified.
Agrochemical composition of southern chernozem and humus content under different farming systems in southern Russia
Authors: Pashtetsky V S , Zubochenko A A , Turin E N , Gongalo A A 
Published: January 2021

Abstract
The purpose of the research is to study the content of various nutrients and the amount of humus using different farming systems (the traditional and direct-sowing farming systems) on chernozem in southern Russia. The stationary experiment was conducted in 2015 (five-field crop rotation). In 2021, the first rotation will end. Agrotechnics corresponding for this region was used. Fertilizer rates were applied in equal amounts. The soil is southern chernozem on loesslike light clays. The climate with pronounced continental features is characterized by a significant change in temperatures during various periods of plant vegetation. The average annual air temperature is 10 °C, the annual precipitation is 428 mm. Sampling was carried out in the first decade of October (the first field of crop rotation). The humus content should be one of the objects of environmental monitoring. It determines quantity and quality of crops and their resistance to dehumification. A tendency toward the accumulation of humus was observed when using the direct sowing method. The use of no-till has a positive effect on humus formation, which makes it possible to obtain large yields of better quality. When using the direct sowing method, the main nutrients (phosphorus and potassium) are less accessible. The ratio of carbon to nitrogen decreases which indicates higher nitrogen content.
Semar Sandy-app: monitoring system for sandy soil irrigation based on android application
Authors: Arifin I , Herawati A , Mujiyo Sumani , Widijanto H 
Published: January 2021

Abstract
Indonesia’s population increase leads to increase in food needs. The increasing need for food requires national food security. However the country faces the problem of agricultural land diminishing. Sandy soil is one of the suboptimal land that can be used for agricultural cultivation. Sandy soil leads to problems such as low water-holding capacity that will result in low levels of soil moisture. Soil moisture content is a very important factor in plant growth. Accordingly, we need an alternative solution to solve the problem. One of technology used in agriculture is an irrigation system. Therefore, the author proposes an idea in forms of Semar Sandy-App. Semar Sandy-App is a monitoring system as well as a system that provides direct supply water in the plant root zone. The system uses subsurface drip irrigation with perforated pipes then water comes out of the hole to wet the soil. This system can be a solution to optimize sandy soil through efficiency and effectiveness in providing water. The system can control and monitor the plant water requirements through the use of microcontroller that is connected directly to the android system, so it will increase agricultural productivity to support the availability of food for the community.
Seasonal variation in hybrid seed adoption: The case of chilli in Indonesia
Authors: Sayekti A L , Nugrahapsari R A , Hasibuan A M 
Published: January 2021

Abstract
Chilli is one of important vegetables in Indonesia, which is produced all year long despite the seasonal variation. While seasonality in consumer demand and producer supply of agricultural commodities is well understood, relatively little is known regarding seasonal variation in adoption of farm technologies, such as hybrid seeds, whose effectiveness may vary across seasons. Hybrid seed adoption studies in developing countries tend to suffer from a problem of temporal aggregation where data is either (1) measured in a snapshot of time, in which case seasonal variation cannot be analysed, or; (2) aggregated over a year or multiple years, where cyclical seasonal variation is washed out. This study uncovers seasonal dynamics in hybrid seed demand by analysing the case of chilli in hybrid seed use by farmers will be critical for improving seed-system. Factor demand functions were estimated using a unique dataset of 597 chilli producers. The study tested for structural change in demand for hybrid seeds going from dry season to wet season and identify season-specific constraints to hybrid seed use. Results showed that hybrid seed adoption is likely associated with water supply, especially during dry seasons. The adoption of hybrid seed could be lower in irrigated land during the rainy season.
In vitro fibroblast cells culture from Pelung chicken embryo and its potential application
Authors: Herawati E , Wulandari H , Listyawati S , Etikawati N 
Published: January 2021

Abstract
The availability of in vitro cell culture derived from local breed provides an opportunity for tackling problems related to the preservation of its genetic materials and can potentially be applied for downstream in vitro-based studies. Here, we established primary fibroblast cell culture from Pelung chicken, then explored its growth characteristic and potential uses for wound healing assay and cytotoxicity tests of medicinal bioactive compounds. Fibroblast cells were isolated from embryonic skin tissue and maintained in DMEM-FBS media. Wound healing assay was performed by creating a “scratch” in the cell monolayer, followed by capturing periodic images of migrating cells. Cell viability was measured using trypan blue dye exclusion assay in various doses of Centella asiatica L. leaf extract. Cells outgrowth from the skin explant revealed a typical morphology of fibroblast-like cells that reached maximum growth at 7.95 × 104 cells/cm2 after 5 days. With continuous passage, the population of the cells became more homogeneous and population doubling time increased. In the wound healing assay, cells migrated towards the wound area within 24 hours, suggesting their ability to normally respond to chemical cues. In cytotoxicity test, cells’ viability corresponded in a dose-dependent manner with the amount of C. asiatica extract tested into the culture.
Managing ecotourism in Genilangit based on a stimulus of religious ecology in order to adapt to climate change
Authors: Lestari E , Sugihardjo  , Wibowo A 
Published: January 2021

Abstract
Climate change has created a sense of global kinship and increased levels of religiosity. Environmental management is essentially based on two factors, namely reducing environmental risk and increasing environmental benefit. This article describes the sustainability of ecotourism developments, the development of ecotourism in Genilangit based on participation, the management of ecotourism in Genilangit based on a stimulus of religious ecology, and the strategies of the mountainside community for adapting to climate change. The research uses a qualitative method with a case study, which aims to gain a deep understanding of the attitudes and behaviour of the community with regard to religious ecology in developing ecotourism. The results of the research show that ecotourism in Genilangit is “Green and Fair” in its concern for sustainable development and conservation, in aspects of economic sustainability, social sustainability, and environmental sustainability. Ecotourism in Genilangit is community based, and the principle of religious ecology is always implemented as the basis for managing ecotourism which is adaptive to climate change. The success of the Genilangit community in adapting to climate change is due to their social and cultural adaptations which are implemented within a framework of religious ecology.
Evaluation of dietary beta-agonist on production performance and carcass of beef cattle: a meta-analysis
Authors: Ridla M , Laconi E B , Nahrowi  , Jayanegara A 
Published: January 2021

Abstract
This study aimed to evaluate the use of beta-agonist on beef cattle by integrating data from various experiments and analyzing the data by a meta-analysis method. A total of 42 articles were used to build a database in which the articles reported dietary addition of beta-agonist on performance and carcass of beef cattle. Treatments were grouped into control (without beta-agonist) and with beta-agonist addition. The treatments were considered as fixed effects whereas different experiments were considered as random effects. Model statistics used was P-value in order to distinguish between the two groups, and the P-value less than 0.05 was considered to be significant. Results showed that dietary beta-agonist addition increased average daily gain and gain to feed ratio of beef cattle (P<0.001). Beta-agonist elevated hot carcass weight (P<0.001) and dressing percentage (P<0.01), but had no effect on fat thickness. Longissimus muscle area increased (P<0.001) while marbling score decreased (P<0.05) due to the addition of beta-agonist. The additive positively influenced Warner-Bratzler shear force of carcass (P<0.001) but had no effect on its pH value. In conclusion, beta-agonist favourably affects production performance and carcass property of beef cattle.
Rice strain selection for Mentik Wangi variety M3 generation resulting from 200 gray gamma irradiation
Authors: Hafsyari S K , Purwanto E , Yunus A 
Published: January 2021

Abstract
Mentik Wangi rice is a local variety that is quite popular with the people of Magelang. Mentik Wangi rice has a distinctive aroma and has a fluffier texture. The disadvantage of Mentik Wangi rice is the potential for lower production and the plants are too high. There is an effort done to overcome that problem were planted mutation through the utilization of gamma-ray irradiation. The aim of this research is to select M3 generation mutant rice resulting from 200 gray gamma-ray irradiation which has a short stems and has higher productivity and determine the performance of M3 generation mutant rice resulting from 200 gray gamma-ray irradiation. The observational data were analyzed descriptively and compared the performance of the Mentik Wangi M3 rice plants as a result of 200 gray gamma-ray irradiation with control plants through T-test analysis. The results showed that the performance of the M3 generation of Mentik Wangi rice was better than control plants, there were several strains with potential mutants traits based on the positive character of each individual. There are 24 Mentik Wangi M3 generation rice plants resulting from 200 gray gamma-ray irradiation plants which are selected based on short stem and have a high productivity.
Simulating allele frequency changes in Indonesian goat crossbreeding scenarios
Authors: Prastowo S , Widowati I F I , Nuraini D M , Widyas N 
Published: January 2021

Abstract
This study was aimed to predict heterozygosity related to body weight (BW) trait from simulation of allele frequency changes in five crossbreeding scenario of Indonesian goat breed, namely Indonesian goat called Jawarandu (Etawah grade ♂ >< Kacang ♀), Boer (Boer ♂ >< Boer ♀), Boerja F1 (Boer ♂ >< Jawarandu ♀), Boerja F2 (Boer ♂ >< Boerja F1♀) and Boerja F3 (Boer ♂ >< Boerja F2 ♀). Allele frequency simulation was conducted, the heterozygosity were estimated and the associations to observed BW were measured from corresponding goat samples. BW (kg) data was retrieved from 1.5-year-old goats from the studied samples. In this study, allele that control BW trait was coded as “B” for high and “b” for low BW. The initial allele frequencies in Jawarandu were set as (B=0.45; b=0.55) and in Boer were (B=0.7; b=0.3). The simulation resulted in allele frequency in Boerja F1 were (B=0.57; b=0.43), F2 (B=0.64; b=0.36) and F3 (B=0.67; b=0.33). The estimated heterozygosity values were 0.54 0.42; 0.52; 0.47 and 0.44 in Jawarandu, Boer, F1, F2 and F3 respectively. Whereas the respective average of observed BW was 30.50±4.50; 49.50±5.90; 41.80±4.10; 41.60±3.60; and 39.80±3.20 kg. We confirmed that crossbreeding changes the heterozygosity in our simulation study.
Reproductive performance comparison between natural and artificial service in Jawarandu goat
Authors: Nuraini D M , Prastowo S , Widyas N 
Published: January 2021

Abstract
This study was aimed to compare reproductive performance of Jawarandu goat serviced naturally and artificially. In total 433 Jawarandu does were randomly allocated into two groups namely Natural Mating (NM; n=233) and Artificial Insemination (AI; n=200). In this study, reproductive performance was measured in percentage of pregnancy, litter size, and kid born single, twin and triplet. Between groups, data were compared using t-test at α = 5%. NM group was performed in mating pen, and per Boer buck was colonized with 20-25 does for 45 days, followed with pregnancy check using ultrasonography at day 45 post insemination. In AI group, oestrous signs were observed in morning and evening, while insemination was done maximum 12 hours after sign of oestrous. The percentage of pregnancy in NM was higher (p<0.05) compared to AI (73.39 vs 21.00%), litter size (1.37±0.49 vs 1.35±0.56; p>0.05). Similarly, in percentage of kid born single, twin and triplet, no differences were found in both groups (63.35 vs 64.86; 36.02 vs 35.14; and 0.62 vs 0%; respectively). All in all, natural service gives better reproductive performance compared to the artificial one. Its suggested due to factors related to the ability to determine the right time for service.
Selection of M5 short-stemmed mutants from 200 gray gamma ray irradiation in Mentik Wangi rice
Authors: Qoni’ah S A , Parjanto  , Yunus A 
Published: January 2021

Abstract
Mentik Wangi rice is native to Indonesia, it has an oval round shape of rice, yellow brown rice, and fragrant aroma. Mentik Wangi has a weakness that is relatively long harvest time, the plant is too high so it easily fell. This research aims to determine the performance of M5 generation of mutant rice and to select M5 generation of mutant plants resulted from 200 gray gamma irradiation which has short stems with high productivity. The research was carried out by planting 36 genotypes, namely 35 M4 lines of 200 gray gamma ray irradiation and control plant (without irradiation). Observation data were analyzed descriptively and t test to determine differences in M5 generation of Mentik Wangi rice from 200 gray gamma ray irradiation with control plants (without irradiation). Based on the research results that Mentik Wangi rice plants with gamma ray irradiation 200 Gray have better performance compared to control plants (without irradiation) and there are 28 individuals M5 Mentik Wangi rice mutants selected by short-stemmed plants and have high productivity.
Genetic variability of Phaius and Dendrobium orchids based on molecular markers
Authors: Yuhanna W L , Hartati S , Sugiyarto  , Marsusi  
Published: January 2021

Abstract
This study aims to assess the diversity and degree of grouping of several types of orchids in Indonesia. Laboratory work was carried out at the Laboratory of the Center for Horticulture and Tropical Studies IPB by analyzing the variability of orchids using RAPD. Plant material is taken from the orchid collection at the Bogor Botanical Gardens. The relationship between 9 orchids was analyzed using binary phenotype data and DNA band patterns as assessed by amplification of 2 random RAPD primers. The cluster analysis was carried out under the NTSYSpc version 2.02 program and the UPGMA SimQual function. The results showed genetic diversity of the genus Phaius and Dendrobium. Based on primers OPA7 and OPA9 with a similarity coefficient of 0.4 divided into 4 groups. Group I consisted of Phaius tankervilliae, Phaius montanus and Phaius callosus. Group II consisted of Dendrobium mirbelianum, Dendrobium lamellatum, Dendrobium liniale and Dendrobium biggibum. Group III consists of Dendrobium anosmum. Group IV consisted of Phaius ambionensis. The conclusion of this study is that there are 4 groups from the genus Phaius and Dendrobium based on the genetic marker RAPD.