bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathologic features and prognostic value of DNA mismatch protein expression patterns in stage Ⅱ/Ⅲ colorectal cancer.
Authors: Shang An , Zhao Zeyun , Wang Zhaoyong , Li Donglin , Guo Yu , Qi Rui , Yang Yang , Wang Min 
Published: November 2021

Abstract
Background

MSI CRCs were associated with better prognosis and limited predictive value for adjuvant chemotherapy. However, whether the same is true in Northeastern China is still unclear. The aim of the present study was to evaluate the association of clinicopathologic features and MMR/MSI status determined with immunohistochemistry analysis in Northeast China patients with stage Ⅱ/Ⅲ CRCs. Particularly, we sought to detect the relationship between MMR/MSI status and efficacy of oxaliplatin and fluoropyrimidine based adjuvant chemotherapy.
Methods

In total, 476 pathological specimens from eligible stage Ⅱ/Ⅲ CRCs were analyzed with IHC between 2016 and 2018, of which 63 CRCs were diagnosed with MMR protein deficiency. Clinicopathological features and overall survival (OS) were compared between these above two groups.
Result

The incidence of dMMR CRCs in our cohort was 13.2% (63/476). Immunohistochemistry (IHC) revealed two common dMMR IHC patterns in 63 dMMR CRCs. And dMMR type1 showed a higher proportion of women (P=0.001) and earlier pathological N stage (P=0.075). In the multivariate Cox regression model, POC (Postoperative chemotherapy) and dMMR were associated with a favor prognosis in CRC patients with stage II/III (HR 0.47, 95%CI 0.30-0.74, P=.001; HR 0.34, 95%CI 0.14-0.79, P=.013). However, adjuvant chemotherapy based on oxaliplatin and fluorouracil cannot prolong the OS of dMMR CRCs (P=0.182).
Conclusions

MMR protein appeared distinct associations with tumor staging, serum CEA level and tumor size. And MMR protein was an independent prognostic marker in patients with stage Ⅱ CRC, whereas dMMR CRC patients did not seem to benefit from oxaliplatin combined with fluorouracil-based adjuvant chemotherapy.
Quality of Life in a Randomized Trial Comparing Two Neoadjuvant Regimens for Locally Advanced Rectal Cancer – INCAGI004.
Authors: Araujo Rodrigo Otavio de Castro , Vieira Fernando Meton , Ornellas Ana Paula Victorino , Torres Claudia Carrada , Martins Ivanir , Guaraldi Simone , Valadão Marcus Vinicuis , Linhares Eduardo , Ferreira Carlos Gil , Thuler Luiz Claudio 
Published: November 2021

Abstract
Background: Neoadjuvant chemoradiotherapy (neoCRT) followed by surgery is the standard of care for locally advanced rectal cancer (LARC), but the emergence of different drug regimens may result in different response rates. Good clinical response translates into greater sphincter preservation, but quality of life (QOL) may be impaired after treatment due to chemoradiotherapy and surgical side effects. Objective: To prospectively evaluate the QOL in a randomized trial comparing two neoadjuvant regimens for locally advanced rectal cancer.Methods: Stage II and III rectal cancer patients were randomized to receive neoCRT with either capecitabine (Group 1) or 5-Fu and leucovorin (Group 2) concomitant to long course radiotherapy. Clinical downstaging was accessed using MRI 6-8 weeks after treatment. EORTCs QLQ C30 and CR38 were applied before treatment (T0), after neoCRT (T1), after rectal resection (T2), early after adjuvant chemotherapy (T3), and one year after end of treatment or stoma closure (T4). Wexner scale was used for continence evaluation at T4. A C30SummaryScore (Geisinger et cols) was calculated to compare QOL results. Results: 32 patients were assigned to Group 1 and 31 to Group 2. Clinical downstaging occurred in 70.0% of Group 1 and 53.3% of Group 2 (p=0.288). pCR was 23.3% in group 1 and 10.0% in Group 2(p=0.165). Sphincter preservation was 83.3% in Group 1 and 80.0% in Group 2(p=0.111). No difference in QOL was detected comparing the two treatment groups before and after neoCRT. C30SummaryScore detected improvement comparing T0 to T1 and deterioration comparing T1 to T2 (p=0.025), and global health status improved at T1 and T4 compared to T0(p=0.004). Mean Wexner scale score was 9.2, and a high score correlated with symptoms of diarrhea and defecation problems at T4.Conclusions: Clinical and pathological response rates were equivalent in both treatment groups. QOL was improved after neoCRT corresponding to clinical response but decreased following rectal resection. Wexner score was high after sphincter preservation. C30SummaryScore was a useful tool to detect differences in overall QOL in EORTCs multiple item questionnaire.Trial registration: NCT03428529. Registered 02/09/2018 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT03428529.
Landscape Characteristics Influence Ranging Behavior of Asian Elephants at The Human-Wildlands Interface in Myanmar
Authors: Chan Aung Nyein , Wittemyer George , McEvoy John , Williams Amirtharaj Christy , Cox Nick , Soe Paing , Grindley Mark , Shwe Nay Myo , Chit Aung Myo , Oo Zaw Min , Leimgruber Peter 
Published: November 2021

Abstract
ContextAsian elephant numbers are declining across much of their range driven largely by serious threats from land use change resulting in habitat loss and fragmentation. Myanmar, holding critical range for the species, is undergoing major developments due to recent sociopolitical changes. To effectively manage and conserve the remaining populations of endangered elephants in the country, it is crucial to understand their ranging behavior.ObjectivesOur objectives were to (1) estimate the sizes of dry, wet and annual ranges of free ranging elephants in Myanmar; and quantify the relationship between dry season (the period when human-elephant interactions are the most likely to occur) range size and configurations of agriculture and natural vegetation within the range, and 2) evaluate how percentage of agriculture within dry core range (50% AKDE range) of elephants relates to their daily distance traveled.MethodsWe used autocorrelated kernel density estimator (AKDE) based on a continuous-time movement modeling (ctmm) framework to estimate dry season (26 ranges from 22 different individuals), wet season (12 ranges from 10 different individuals), and annual range sizes (8 individuals), and reported the 95%, 50% AKDE, and 95% Minimum Convex Polygon (MCP) range sizes. We assessed how landscape characteristics influenced range size based on a broad array of 48 landscape metrics characterizing aspects of vegetation, water, and human features and their juxtaposition in the study areas. To identify the most relevant landscape metrics and simplify our candidate set of informative metrics, we relied on exploratory factor analysis and Spearman’s rank correlation coefficient. Based on this analysis we adopted a final set of metrics into our regression analysis. In a multiple regression framework, we developed candidate models to explain the variation in AKDE dry season range sizes based on the previously identified, salient metrics of landscape composition. ResultsElephant dry season ranges were highly variable averaging 792.0 km2 and 184.2 km2 for the 95% and 50% AKDE home ranges, respectively. We found both the shape and spatial configuration of agriculture and natural vegetation patches within an individual elephant’s range play a significant role in determining the size of its range. We also found that elephants are moving more (larger energy expenditure) in ranges with higher percentages of agricultural area.ConclusionOur results provide baseline information on elephant spatial requirements and the factors affecting them in Myanmar. This information is important for advancing future land use planning that takes into account space-use requirements for elephants. Failing to do so may further endanger already declining elephant populations in Myanmar and across the species’ range.
Resolvin D1 inhibits endothelial permeability and mitochondrial damage following cardiac ischemia reperfusion in diabetic mice
Authors: Zhang Jialiang , Huang Fangyang , Chen Li , Li Guoyong , Xiao Dan , Lei Wenhua , Zhao Jiahao , Liao Yanbiao , Li Changming , Chen Mao 
Published: November 2021

Abstract
Purpose

Novel strategies for preventing myocardial ischemia reperfusion injury (MIRI) in a diabetic heart are urgently needed. Resolvin D1 (RvD1) plays important therapeutic roles in inflammatory diseases. However, the therapeutic role of RvD1 in diabetic MIRI is still unknown.
Methods

Diabetic mice were established with a high-fat diet and streptozotocin (STZ). The mice were pretreated with RvD1 via intraperitoneal injection for 3 days, followed by MIRI. To evaluate the effects of RvD1 on chronic cardiac remodelling, RvD1 was administered for another 2 weeks after MIRI. The effects of RvD1 following MIRI were measured, including the severity of infarct size, regional inflammation, cardiac function, and permeability of cultured endothelial monolayers. Mitochondrial reactive oxygen species (MitoROS) and mitochondrial membrane potential (MMP) were determined using MitoSOX and JC-1.
Results

RvD1 pretreatment significantly reduced infarct size and the Evans blue content in diabetic injured hearts, which was associated with improved endothelial permeability. At 2 weeks after MIRI, RvD1 treatment partially improved cardiac performance and reduced cardiac fibrosis in diabetic MIRI mice. In vitro, RvD1 attenuated endothelial leakage induced by hypoxia-reoxygenation, H2O2, and lipopolysaccharide (LPS) under high glucose (HG) conditions. Meanwhile, RvD1 remarkably protected endothelial cells from H2O2-induced mitochondrial damage, as evidenced by increased MMP and decreased MitoROS, which was associated with the preservation of VE-cadherin.
Conclusion

RvD1 alleviates MIRI-induced endothelial permeability and mitochondrial damage injuries in diabetic hearts. Therefore, RvD1 could be a potential therapeutic target for MIRI in diabetes.
Left Atrial Appendage Morphology With The Progression of Atrial Fibrillation
Authors: Takaya Yoichi , Nakayama Rie , Yokohama Fumi , Toh Norihisa , Nakagawa Koji , Miyamoto Masakazu , Ito Hiroshi 
Published: November 2021

Abstract
Left atrial appendage (LAA) size is crucial for determining the indication of transcatheter LAA closure. The aim of this study was to evaluate the differences in LAA morphology according to the types of atrial fibrillation (AF). A total of 340 patients (mean age: 65 ± 15 years) who underwent transesophageal echocardiography (TEE) were included. Patients were classified into non-AF (n = 105), paroxysmal AF (n = 86), persistent AF (n = 87), or long-standing persistent AF (n = 62). LAA morphology, including LAA ostial diameter and depth, was assessed using TEE. Patients with long-standing persistent AF had larger LAA ostial diameter and depth, greater LAA lobes, and lower LAA flow velocity. The maximum LAA ostial diameter was 19 ± 4 mm in patients with non-AF, 21 ± 4 mm in patients with paroxysmal AF, 23 ± 5 mm in patients with persistent AF, and 26 ± 5 mm in patients with long-standing persistent AF. LAA ostial diameter was increased by 2 or 3 mm with the progression of AF. LAA ostial diameter was correlated with LA volume index (r = 0.37, p < 0.01) and the duration of continuous AF (r = 0.30, p < 0.01), but not with age or the period from the onset of AF. In conclusion, LAA size, which is the determinant for selecting device size of transcatheter LAA closure, was increased with the progression of AF. Our findings have potential implications for therapeutic strategy of transcatheter LAA closure.
Effect of Intradermal Thumbtack Needle for Functional Constipation: Study Protocol for a Real- World Clinical Trial
Authors: Zhao WenQian , Xu Ying , Liao Jingjing , Li Ji , Wang Li , Cao Jingkui , Tang Yishi , Gu Jirui , Li Dehua 
Published: November 2021

Abstract
Background: Many high-quality clinical trials have proved the efficacy of acupuncture in the improving frequency of spontaneous bowel movements, stool characteristics, and et cetera of Functional Constipation (FC) [1]. However, the high requirement of time make many patients unable to attend, this clinical trial will demonstrate the efficacy of intradermal thumbtack needle in ameliorate spontaneous bowel movement of FC.Methods: This multi-center real world clinical trial is performed involving 482 FC patients. All patients are randomly allocated into 2 group, which are group A to receive intradermal thumbtack needle twice a week for 4 weeks (intervention group n=241) or group B to take oral administration of mosapride 3 times a day for 4 weeks (control group n=241). This trial includes a 4-week treatment period and a 4-week follow-up period. The primary outcome is the number of completely spontaneous defecation per week, we use Weekly CSBMs analyze the frequency of spontaneous defecation per week during treatment and follow-up period to compared with the baseline. The secondary outcomes include FC patients’ stool consistence, gastrointestinal or anorectal symptoms, quality of life, anxiety and depression levels during treatment and follow-up period and efficacy expectations. The relevant assessment tools include: Bristol tool Form Scale (BSFS), Cleveland Constipation Score (CCS), Patient Assessment of Constipation Quality of Life Questionnaire(PAC－QOL), Self-rating Anxiety Scale (SAS) , Self-rating Depression Scale (SDS) and Efficacy Expectancy Scale. At the end, the scales filled by patients and researchers will be entered by data administrator, all the outcome assessments will be performed by independent outcome assessors and will be analyzed by professional statisticians with SPSS 25.0 software.Discussion: We hypothesize that intradermal needle is better than or equal to positive drugs in the improving frequency of spontaneous bowel movements, stool characteristics，and et cetera of FC patients . The results of this trial will provide us with a clinical basis for the application of intradermal needles in the treatment of FC.Trial registration: Chinese Clinical Trial Registry, ChinCTR2100043684.Registered on 26 February 2021.
Predictors for Development of Oxaliplatin-induced Peripheral Neuropathy in Cancer Patients as Determined by Ordered Logistic Regression Analysis
Authors: Kanbayashi Yuko , Ishikawa Takeshi , Kuriu Yoshiaki , Tabuchi Yusuke , Otsuji Eigo , Takayama Koichi 
Published: November 2021

Abstract
Purpose This retrospective study aimed to identify predictors for the development of oxaliplatin-induced peripheral neuropathy (OXAIPN). Methods Between January 2017 and March 2021, a total 322 cancer patients at our hospital who were receiving oxaliplatin were enrolled. For the regression analysis of factors associated with oxaliplatin-induced peripheral neuropathy, variables were extracted manually from medical charts. The level of OXAIPN was evaluated using the National Cancer Institute’s Common Terminology Criteria for Adverse Events (version 5). Multivariate ordered logistic regression analysis was performed to identify predictors for the development of OXAIPN. Optimal cut-off thresholds were determined using receiver operating characteristic (ROC) analysis. Values of P <0.05 (2-tailed) were considered significant. Results Significant factors identified included body mass index (BMI) (odds ratio [OR] = 1.06, 95% confidence interval [CI] = 1.00–1.12; P = 0.046), number of cycles (OR = 1.09, 95%CI = 1.05–1.14; P <0.0001), S-1 plus oxaliplatin (SOX) regimen (OR = 0.54, 95%CI = 0.32–0.92; P = 0.023), concomitant use of proton pump inhibitors (PPIs) (OR = 1.64, 95%CI = 1.05–2.58; P = 0.031) and concomitant use of analgesic adjuvant (OR = 3.30, 95%CI = 1.09–9.97; P = 0.035). Conclusion BMI, number of cycles, SOX regimen, concomitant use of PPIs and concomitant use of analgesic drugs were identified as significant predictors for the development of OXAIPN.
PROM SSCOL – Impact of a Papillomavirus Vaccination Promotion Program in Middle School: A Study Protocol for a Randomized Controlled Trial
Authors: TRAN Phuong Lien , Chirpaz Emmanuel , Boukerrou Malik , Bertolotti Antoine 
Published: November 2021

Abstract
Background: In Reunion Island, incidence and mortality for uterine cervical cancer is high, yet coverage rate for HPV vaccination is low. The main objective of the study is to evaluate, the impact of a health promotion program, promoting HPV vaccination, on the proportion of middle school girls who complete the full HPV vaccination schedule (2 or 3 doses) by the end of school year.Methods: This study is a cluster controlled intervention study using a superiority design. A combined health promotion program will be offered, containing: information to students and parents, training of general practitioners and free school-based vaccination (in a "health bus"). Children who will benefit from this program will constitute the intervention group, and will be compared to children from another middle school who will not benefit from any program, constituting the control group.Discussion: Our hypotheses were as follow : Clear and appropriate information for the target population as well as for their parents, will improve their knowledge about HPV vaccination, and thus increase their adherence to this vaccination ; combining information with vaccination in the school setting will reduce any material obstacles that may prevent the vaccination process; raising awareness among general practitioners will enable them to better understand the benefits and risks of HPV vaccination, and thus encourage families, who naturally trust them, to adhere to the program. Final implication would be an extension of this program in all middle schools of the Island and thus increase HPV vaccination coverage.Trial registration: ClinicalTrials.gov ID: NCT04459221
Use of a New Off-the-shelf 3D-printed Trabecular Titanium Acetabular Cup in Chinese Patients Undergoing Hip Revision Surgery: Short- to Mid-term Clinical and Radiological Outcomes
Authors: Shang Guangqian , Xiang Shuai , Guo Cuicui , Guo Jianjun , Wang Peng , Wang Yingzhen , Xu Hao 
Published: November 2021

Abstract
Background: Revision total hip arthroplasty (THA) has been a challenge for surgeons. The purpose of this study was to explore the short-to mid-term clinical and radiological outcomes of Chinese patients who underwent revision THA using a new off-the-shelf three-dimensional (3D)-printed trabecular titanium (TT) acetabular cup by comparison with a conventional porous coated titanium acetabular cup, to provide a reference for the recommendation of this prostheses.Methods: A retrospective analysis of 57 patients (57 hips) who received revision THA was performed from January 2016 to June 2019. A total of 23 patients received 3D-printed cups (observation group) and 34 patients received non-3D-printed cups (control group). Clinical scores including Visual Analogue Scale (VAS), Harris Hip Score (HHS) and Short Form 36 (SF-36), upward movement of the hip center of rotation（HCOR）and limb-length discrepancy (LLD), stabilization and bone ingrowth of cups were compared between two groups. The multivariate linear regression was used to determine the factors potentially influencing the HHS score. Postoperative complications in the two groups were also recorded. Results: All 57 patients were routinely followed up. The average follow-up durations in the control and observation groups were 43.57 ± 13.68 (24–65) months and 41.82 ± 11.44 (24–64) months, respectively (p = 0.618). The postoperative clinical scores significantly improved in both groups compared to the preoperative scores (p < 0.05). The VAS score did not significantly differ between the groups at 3 or 12 months postoperatively, or at the last follow-up (p > 0.05). The HHS and SF-36 scores did not significantly differ between the groups at 3 months postoperatively (p > 0.05) but differed at 12 months postoperatively and the last follow-up (p < 0.05). Compared with the control group, the postoperative recovery of HCOR and LLD was better in the observation group (p < 0.05). All cups remained stable, with no loosening throughout the follow-up period. But the observation group had a significantly better rate of bone ingrowth compared to the control group (p = 0.037). Multivariate linear regression analysis showed that different cup types, upward movement of the HCOR, and LLD influenced the HHS score at the last follow-up (p < 0.05). None of the patients exhibited severe postoperative complications.Conclusion: The new off-the-shelf 3D-printed TT acetabular cup demonstrated encouraging short-to mid-term clinical outcomes in Chinese patients. It can effectively relieve pain, improve hip function, provide satisfactory biological fixation and high survival rate. But further follow up is necessary to assess its long-term outcomes.
Evidence Synthesis Methods to Estimate Subgroup Effects for Distributional Cost-effectiveness Analysis of New Treatments
Authors: Jansen Jeroen P 
Published: November 2021

Abstract
Distributional cost-effectiveness analysis (DCEA) is an extension of conventional cost-effectiveness analysis to quantify health equity impacts. Although health disparities are recognized as an important concern, the typical analyses conducted to inform health technology assessment of a new intervention do not include a DCEA. One of the reasons brought forward is the relative sparseness of the available evidence for a new intervention. The objective of this paper is to review advanced evidence synthesis methods to estimate subgroup specific treatment effects relevant for a DCEA of new interventions. The paper will outline the evidence needs and gaps, present alternative evidence synthesis methods followed by an illustrative example, and conclude with some practical recommendations. Evidence challenges for estimating relative treatment effects are due to lack of inclusion of relevant subgroups in the randomized controlled trials (RCTs), lack of access to individual patient data, small subgroups resulting in uncertain effects, and reporting gaps. Evidence synthesis methods can help overcome evidence gaps by considering all relevant direct, indirect, and external evidence simultaneously. Methods of potential relevance include (network) meta-analysis with shrinkage estimation, conventional (network) meta-regression analysis, multi-level (network) meta-regression analysis, and generalized evidence synthesis. For a new intervention for which only RCT evidence is available and no real-world data, estimates can be improved if the assumption of exchangeable subgroup effects or the shared or exchangeable effect-modifier assumption among competing interventions can be defended. Future research is needed to assess the pros and cons of different methods for different data gap scenarios.
Androgen Receptor Expression Associates With Distinctive Clinicopathological and Molecular Features in ER-Positive and ER-Negative Breast Cancer
Authors: Hu Taobo , Liu Yiqiang , Zhao Guiyang , Wang Shu , Long Mengping 
Published: November 2021

Abstract
Background: Androgen receptor (AR) expression is frequently observed in breast cancer, but its association with estrogen receptor (ER) expression of breast cancer remains unclear. Methods: In this study, we analyzed the clinicopathological and molecular features associated AR loss in ER-positive and ER-negative breast cancer respectively, trying to elucidate the molecular correlation between AR and ER. Results: Our results showed that AR loss was associated with different clinicopathological characteristics in ER-positive and ER-negative breast cancer. Moreover, the expression of AR was correlated with different molecular features in ER-positive and ER-negative breast cancer.Conclusions: These results suggest that the role of AR in ER-positive breast cancer is distinctive from that in ER-negative breast cancer.