bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crowdsourced Tick Image-Informed Updates to U.S. County Records of Three Medically Important Tick Species
Authors: Kopsco Heather L , Duhaime Roland J , Mather Thomas N 
Published: May 2021

AbstractBurgeoning cases of tick-borne disease present a significant public health problem in the United States. Passive tick surveillance gained traction as an effective way to collect epidemiologic data, and in particular, photograph-based tick surveillance can complement in-hand tick specimen identification to amass distribution data and related encounter demographics. We compared the Federal Information Processing Standards (FIPS) code of tick photos submitted to a free public identification service (TickSpotters) from 2014 to 2019 to published nationwide county reports for three tick species of medical concern: Ixodes scapularis Say (Ixodida: Ixodidae), Ixodes pacificus Cooley and Kohls (Ixodida: Ixodidae), and Amblyomma americanum Linneaus (Ixodida: Ixodidae). We tallied the number of TickSpotters submissions for each tick species according to “Reported” or “Established” criteria per county, and found that TickSpotters submissions represented more than half of the reported counties of documented occurrence, and potentially identified hundreds of new counties with the occurrence of these species. We detected the largest number of new county reports of I. scapularis presence in Michigan, North Carolina, and Texas. Tick image submissions revealed potentially nine new counties of occurrence for I. pacificus, and we documented the largest increase in new county reports of A. americanum in Kentucky, Illinois, Indiana, and Ohio. These findings demonstrate the utility of crowdsourced photograph-based tick surveillance as a complement to other tick surveillance strategies in documenting tick distributions on a nationwide scale, its potential for identifying new foci, and its ability to highlight at-risk localities that might benefit from tick-bite prevention education.
Brahms’s Hungarian Dances and the Early ‘Csárdás’ Recordings
Authors: Banks Jon 
Published: May 2021

Abstract
The relationship of Brahms’s Hungarian Dances to their origins has been a subject of enquiry ever since they were first published. This article extends previous investigations of Brahms’s written sources to include the many 78rpm recordings of ‘traditional’ Hungarian music made shortly after his death. It establishes that nearly half of the Brahms melodies appear in the repertory of popular recording artists such as the so-called ‘Gypsy bands’, and that many of these artists had been active in Vienna during Brahms’s lifetime. It identifies other performing contexts—military bands, popular songs, salon pieces—and argues that the ‘Hungarian Dances’ are an invented amalgamation of several distinct genres, reflecting Brahms’s urban experience and musical interests. The article compares Brahms’s arrangements with the same tunes in oral tradition where possible and considers the wider implications of the recorded legacy for our understanding of the ‘style hongrois’ and Brahms’s appropriation of it.
Hamilton’s Object – a clumpy galaxy straddling the gravitational caustic of a galaxy cluster: constraints on dark matter clumping
Authors: Griffiths Richard E , Rudisel Mitchell , Wagner Jenny , Hamilton Timothy , Huang Po-Chieh , Villforth Carolin 
Published: May 2021

ABSTRACT
We report the discovery of a ‘folded’ gravitationally lensed image, ‘Hamilton’s Object’, found in a HST image of the field near the active galactic nucleus SDSS J223010.47-081017.8 (which has redshift 0.62). The lensed images are sourced by a galaxy at a spectroscopic redshift of 0.8200 ± 0.0005 and form a fold configuration on a caustic caused by a foreground galaxy cluster at a photometric redshift of 0.526 ± 0.018 seen in the corresponding Pan-STARRS PS1 image and marginally detected as a faint ROSAT All-Sky Survey X-ray source. The lensed images exhibit properties similar to those of other ‘folds’ where the source galaxy falls very close to or straddles the caustic of a galaxy cluster. The folded images are stretched in a direction roughly orthogonal to the critical curve, but the configuration is that of a tangential cusp. Guided by morphological features, published simulations and similar ‘fold’ observations in the literature, we identify a third or ‘counter’-image, confirmed by spectroscopy. Because the fold-configuration shows highly distinctive surface brightness features, follow-up observations of microlensing or detailed investigations of the individual surface brightness features at higher resolution can further shed light on kpc-scale dark matter properties. We determine the local lens properties at the positions of the multiple images according to the observation-based lens reconstruction of Wagner. The analysis is in accordance with a mass density which hardly varies on an arcsecond scale (6 kpc) over the areas covered by the multiple images.
Nicotine Delivery and User Ratings of IQOS Heated Tobacco System Compared With Cigarettes, Juul, and Refillable E-Cigarettes
Authors: Phillips-Waller Anna , Przulj Dunja , Pesola Francesca , Smith Katie Myers , Hajek Peter 
Published: May 2021

Abstract

Introduction
Reduced-risk nicotine products are more likely to replace smoking if they match cigarettes in nicotine delivery and user satisfaction.

Aims and Methods
We examined the nicotine delivery profile and user ratings of IQOS heated tobacco system and compared it with own brand cigarettes (OBC), Juul, and refillable e-cigarettes (EC).Participants (N = 22) who were daily vapers smoking &lt;1 cigarette per day on average, attended after overnight abstinence from smoking and vaping, to test at separate sessions OBC, IQOS, and Juul. Eight participants also tested two refillable EC using e-liquid with 20 mg/mL nicotine. At each session, a baseline blood sample was taken before participants used the product ad libitum for 5 minutes. Further samples were taken at 2, 4, 6, 8, 10, and 30 minutes. Maximum nicotine concentration (Cmax), time to Cmax (Tmax), and nicotine delivered over 30 minutes (AUC0–&gt;30) were calculated. Participants rated their urge to smoke and product characteristics.

Results
IQOS delivered less nicotine than OBC (AUC0–&gt;30: z = −2.73, p = .006), and than Juul (AUC0–&gt;30: z = −3.08, p = .002; Cmax: z = −2.65, p = .008), and received less favorable ratings than Juul (effect on urges to smoke: z = −3.23, p = .001; speed of urge relief: z = −2.75, p = .006; recommendation to friends: z = −2.45, p = .014). Compared with refillable EC, IQOS delivered nicotine faster (Tmax: z = −2.37, p = .018), but received less favorable overall ratings (recommended to friends: z = −2.32, p = .021).

Conclusions
IQOS’ pharmacokinetic profile suggests that it may be less effective than Juul for smoking cessation, but at least as effective as refillable EC; although participants, who were experienced vapers rather than IQOS users, preferred refillable EC.

Implications
Because IQOS provided less efficient nicotine delivery than cigarettes and Juul in this sample, and also had a weaker effect on urges to smoke than Juul, it could be less helpful than Juul in assisting such dual users, and possibly smokers generally, to switch to an alternative product. IQOS, however, provided nicotine faster than refillable EC products, although participants preferred EC.
Streptococcus pyogenes Infective Endocarditis—Association With Injection Drug Use: Case Series and Review of the Literature
Authors: Rebechi Melanie T , Heil Emily L , Luethy Paul M , Schmalzle Sarah A 
Published: May 2021

Abstract

Background
Streptococcus pyogenes, or Group A Streptococcus (GAS), is not considered a typical cause of infective endocarditis (IE), but has anecdotally been observed in unexpectedly high rates in people who inject drugs (PWID) at our institution.

Methods
All cases of possible or definite GAS IE per Modified Duke Criteria in adults at an academic hospital between 11/15/2015 and 11/15/2020 were identified. Medical records were reviewed for demographics, comorbidities, treatment, and outcomes related to GAS IE. The literature on cases of GAS IE was reviewed.

Results
Eighteen cases of probable (11) or definite (7) GAS IE were identified; the mean age was 38 years, and the population was predominantly female (56%) and Caucasian (67%), which is inconsistent with local population demographics. Sixteen cases were in people who inject drugs (PWID; 89%); 14 were also homeless, 6 also had HIV (33%), and 2 were also pregnant. Antibiotic regimens were variable due to polymicrobial bacteremia (39%). One patient underwent surgical valve replacement. Four patients (22%) died due to complications of infection. The literature review revealed 42 adult cases of GAS IE, only 17 of which were in PWID (24%).

Conclusions
The 16 cases of possible and definite GAS IE in PWID over a 5-year period in a single institution reported nearly doubles the number of cases in PWID from all previous reports. This suggests a potential increase in GAS IE particularly in PWID and PWH, which warrants further epidemiologic investigation.
Latent Tuberculosis Infection Status of Pregnant Women in Uganda Determined Using QuantiFERON TB Gold-Plus
Authors: Bongomin Felix , Ssekamatte Phillip , Nattabi Gloria , Olum Ronald , Ninsiima Sandra , Kyazze Andrew Peter , Nabakka Winnie , Kukunda Rebecca , Cose Stephen , Kibirige Davis , Batte Charles , Kaddumukasa Mark , Kirenga Bruce J , Nakimuli Annettee , Baluku Joseph Baruch , Andia-Biraro Irene 
Published: May 2021

Abstract

Background
The risk of progression of latent tuberculosis infection (LTBI) to active disease increases with pregnancy. This study determined the prevalence and risk factors associated with LTBI among pregnant women in Uganda.

Methods
We enrolled 261 pregnant women, irrespective of gestational age. Participants who had known or suspected active tuberculosis (TB) on the basis of clinical evaluation or who had recently received treatment for TB were excluded. LTBI was defined as an interferon-γ concentration ≥0.35 IU/mL (calculated as either TB1 [eliciting CD4+ T-cell responses] or TB2 [eliciting CD8+ T-cell responses] antigen minus nil) using QuantiFERON TB Gold-Plus (QFT-plus) assay.

Results
LTBI prevalence was 37.9% (n = 99) (95% confidence interval [CI], 32.3–44.0). However, 24 (9.2%) subjects had indeterminate QFT-plus results. Among participants with LTBI, TB1 and TB2 alone were positive in 11 (11.1%) and 18 (18.2%) participants, respectively. In multivariable analysis, human immunodeficiency virus (HIV) infection (adjusted odds ratio [aOR], 4.4 [95% confidence interval {CI}, 1.1–18.0]; P = .04) and age 30–39 years (aOR, 4.0 [95% CI, 1.2–12.7]; P = .02) were independently associated with LTBI. Meanwhile, smoking status, alcohol use, nature of residence, crowding index, and TB contact were not associated with LTBI.

Conclusions
Our findings are in keeping with the evidence that HIV infection and advancing age are important risk factors for LTBI in pregnancy. In our setting, we recommend routine screening for LTBI and TB preventive therapy among eligible pregnant women.
The emerging role of group 3 innate lymphoid cells in the neonate: interaction with the maternal and neonatal microbiome
Authors: Mirpuri Julie 
Published: May 2021

Abstract
Innate lymphoid cells (ILCs) are critical for host defense and are notably important in the context of the newborn when adaptive immunity is immature. There is an increasing evidence that development and function of group 3 ILCs (ILC3) can be modulated by the maternal and neonatal microbiome and is involved in neonatal disease pathogenesis. In this review, we explore the evidence that supports a critical role for ILC3 in resistance to infection and disease pathogenesis in the newborn, with a focus on microbial factors that modulate ILC3 function. We then briefly explore opportunities for research that are focused on the fetus and newborn.
ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response
Authors: Pascual Jesús , Rahikainen Moona , Angeleri Martina , Alegre Sara , Gossens Richard , Shapiguzov Alexey , Heinonen Arttu , Trotta Andrea , Durian Guido , Winter Zsófia , Sinkkonen Jari , Kangasjärvi Jaakko , Whelan James , Kangasjärvi Saijaliisa 
Published: May 2021

Abstract
Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Posttranslational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3S91D-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against ultraviolet B (UV-B) or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.
Alexithymia and somatization in agenesis of the corpus callosum
Authors: Paul Lynn K , Pazienza Shawneen R , Brown Warren S 
Published: May 2021

Abstract
Deficient communication between the cerebral hemispheres is one of several prevailing neurobiological explanations for alexithymia and has been strongly supported by research on patients with commissurotomy. We examined self-reported symptoms of alexithymia in adults with agenesis of the corpus callosum (AgCC), a condition characterized by more subtle reductions in interhemispheric transfer than in commissurotomy. Sixteen adults with AgCC and full-scale intelligence quotient &gt;80 were compared with 15 neurotypical controls group-matched for age and intelligence score. The AgCC group endorsed greater difficulty identifying and describing feelings and more vague physical symptoms than controls but similar levels of emotional experience and emotional coping. This finding of impaired emotional interpretation with intact emotional experience is consistent with findings in callosotomy patients, implicating the critical role of the corpus callosum in cognitive dimensions of emotion processing. Further study of alexithymia in AgCC using task-based measures may help clarify the nature of this relationship.
Timely N-Acetyl-Cysteine and Environmental Enrichment Rescue Oxidative Stress-Induced Parvalbumin Interneuron Impairments via MMP9/RAGE Pathway: A Translational Approach for Early Intervention in Psychosis
Authors: Dwir Daniella , Cabungcal Jan-Harry , Xin Lijing , Giangreco Basilio , Parietti Enea , Cleusix Martine , Jenni Raoul , Klauser Paul , Conus Philippe , Cuénod Michel , Steullet Pascal , Do Kim Q 
Published: May 2021

Abstract
Research in schizophrenia (SZ) emphasizes the need for new therapeutic approaches based on antioxidant/anti-inflammatory compounds and psycho-social therapy. A hallmark of SZ is a dysfunction of parvalbumin-expressing fast-spiking interneurons (PVI), which are essential for neuronal synchrony during sensory/cognitive processing. Oxidative stress and inflammation during early brain development, as observed in SZ, affect PVI maturation. We compared the efficacy of N-acetyl-cysteine (NAC) and/or environmental enrichment (EE) provided during juvenile and/or adolescent periods in rescuing PVI impairments induced by an additional oxidative insult during childhood in a transgenic mouse model with gluthation deficit (Gclm KO), relevant for SZ. We tested whether this rescue was promoted by the inhibition of MMP9/RAGE mechanism, both in the mouse model and in early psychosis (EP) patients, enrolled in a double-blind, randomized, placebo-controlled clinical trial of NAC supplementation for 6 months. We show that a sequential combination of NAC+EE applied after an early-life oxidative insult recovers integrity and function of PVI network in adult Gclm KO, via the inhibition of MMP9/RAGE. Six-month NAC treatment in EP patients reduces plasma sRAGE in association with increased prefrontal GABA, improvement of cognition and clinical symptoms, suggesting similar neuroprotective mechanisms. The sequential combination of NAC+EE reverses long-lasting effects of an early oxidative insult on PVI/perineuronal net (PNN) through the inhibition of MMP9/RAGE mechanism. In analogy, patients vulnerable to early-life insults could benefit from a combined pharmacological and psycho-social therapy.
The modulatory impacts of Glycyrrhiza glabra extract against methotrexate-induced testicular dysfunction and oxidative stress
Authors: Aldhahrani Adil , Soliman Mohamed Mohamed , Althobaiti Fayez , Alkhedaide Adel , Nassan Mohamed Abdo , Mohamed Wafaa Abdou , Youssef Gehan B A , Said Alshaimaa Mohammed 
Published: May 2021

Abstract
Glycyrrhiza glabra root (licorice) is a widely used herb for its beneficial effects on health. This study explored the protective effects of licorice extract against oxidative stress and testicular dysfunction caused by methotrexate (MTX). Mice were allocated into (i) negative control group that received saline; (ii) licorice extract group, orally administered with 200 mg/kg body weight (bw) licorice extract for 12 days; (iii) positive MTX-intoxicated group, injected with a single intraperitoneal dose of MTX (20 mg/kg bw) on day 7; and (iv) a protective group that received licorice extract for 12 days and then MTX on day 7 as in groups 2 and 3. Total proteins, albumin, globulins, malondialdehyde, glutathione peroxidase, reduced glutathione, IL-1, and IL-6 were measured in blood and testis samples collected from all groups. Testicular oxidative stress, serum reproductive hormones, and spermogram were examined. The expression of steroidogenesis-associated genes (translocator protein; and P450scc) was examined by quantitative real-time PCR. Bcl-2-associated X protein and cyclogenase-2 genes were examined by immunohistochemical analysis. The bioactive contents of licorice extract were confirmed by gas chromatography–mass spectrometry analysis. Pretreatment with licorice extract ameliorated the toxic effects of MTX on total proteins, albumin, and globulins and oxidative stress biomarkers and reversed the effect of MTX on examined serum and tissue antioxidants. Besides, MTX down-regulated mRNA expression of translocator protein and P450scc genes. Licorice extract averted the decrease in serum testosterone and the increase in IL-1β and IL-6 levels induced by MTX. Moreover, MTX increased sperm abnormalities and percentage of dead sperms and reduced sperm motility. These changes were absent in the licorice preadministered group. Licorice prevented the increase in immunoreactivity of testis for Bcl-2-associated X protein and cyclogenase-2 that were overexpressed in MTX-injected mice. Licorice extracts positively regulated the expression of steroidogenesis genes suppressed by MTX, increased antioxidant enzymes (glutathione peroxidase, reduced glutathione, and catalase) and reduced biomarker of oxidative stress (testicular malondialdehyde) and inflammatory cytokines (IL-1 and -6). Moreover, reduction in testicular tissue immunoreactivity to Bcl-2-associated X protein and cyclogenase-2. In conclusion, licorice extract mitigated the toxic effects of MTX-induced testicular dysfunction at biochemical, molecular, and cellular levels.
Proteomic insights into the photosynthetic divergence between bark and leaf chloroplasts in Salix matsudana
Authors: Liu Junxiang , Sun Chao , Zhai Fei-Fei , Li Zhenjian , Qian Yongqiang , Gu Lin , Sun Zhenyuan 
Published: May 2021

Abstract
Bark chloroplasts play important roles in carbon balancing by recycling internal stem CO2 into assimilated carbon. The photosynthetic response of bark chloroplasts to interior stem environments has been studied recently in woody plants. However, the molecular regulatory mechanisms underlying specific characteristics of bark photosynthesis remain unclear. To address this knowledge gap, differences in the structure, photosynthetic activity and protein expression profiles between bark and leaf chloroplasts were investigated in Salix matsudana in this study. Bark chloroplasts exhibited broader and lower grana stacks and higher levels of starch relative to leaf chloroplasts. Concomitantly, decreased oxygen evolution rates and decreased saturated radiation point were observed in bark chloroplasts. Furthermore, a total of 293 differentially expressed proteins (DEPs) were identified in bark and leaf chloroplast profile comparisons. These DEPs were significantly enriched in photosynthesis-related biological processes or Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with photosynthesis. All 116 DEPs within the KEGG pathways associated with photosynthesis light reactions were downregulated in bark chloroplasts, including key proteins responsible for chlorophyll synthesis, light energy harvesting, nonphotochemical quenching, linear electron transport and photophosphorylation. Interestingly, seven upregulated proteins involved in dark reactions were identified in bark chloroplasts that comprised two kinds of malic enzymes typical of C4-type photosynthesis. These results provide comprehensive proteomic evidence to understand the low photochemical capability of bark chloroplasts and suggest that bark chloroplasts might fix CO2 derived from malate decarboxylation.
Subcutaneous mycoses in Ethiopia: a retrospective study in a single dermatology center
Authors: Abate Dagim A , Ayele Mesfin H , Mohammed Amel B 
Published: May 2021

Abstract

Background
The magnitude of various types of subcutaneous mycoses in Ethiopia is unknown.

Methods
We performed a 5-y retrospective review of confirmed cases at ALERT hospital dermatology clinics.

Result
Confirmed cases of subcutaneous mycoses included chromoblastomycosis (n=12) and mycetoma (n=8). The patients originated from four regions: Oromia (n=10), Amhara (n=6), Addis Ababa (n=3) and South (n=1). Males were affected in 75% of cases (15/20). Ages ranged from 19 to 66 y with a median age of 44 y. Duration of disease ranged from 1 to 25 y. Extremities were affected in all cases, with lower limb involvement in 85% (17/20).

Conclusion
Chromoblastomycosis was the most frequent subcutaneous mycosis followed by mycetoma.