bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast Cancer Outcomes Following Immediate Breast Reconstruction with Implants Versus Autologous Flaps: A Propensity Score-Matched Study
Authors: Wu Zhen-Yu , Han Jing , Kim Hee Jeong , Lee Jong Won , Chung Il Yong , Kim Jisun , Lee Sae byul , Son Byung-Ho , Han Hyun Ho , Eom Jin Sup , Jeong Jae Ho , Gong Gyungyub , Kim Hak Hee , Ahn Sei-Hyun , Ko Beom Seok 
Published: April 2021

Abstract
Purpose
A large proportion of patients with breast cancer who had mastectomy had undergone breast reconstruction with implants or autologous flaps. However, only a few studies have compared the breast cancer outcomes between the implant-based reconstruction (IBR) and autologous flap reconstruction (AFR). In this study, we retrospectively compared the local recurrence rates, distant metastasis rates, and survival outcomes between immediate IBR and AFR.
Methods
A total of 1530 patients with primary breast cancer who underwent IBR or AFR with nipple-/skin-sparing mastectomy were included. Patients who underwent neoadjuvant systemic therapy were excluded from the study. After propensity score matching by age at diagnosis, T stage, N stage, molecular subtype, mastectomy type, adjuvant radiotherapy status, and follow-up period, 938 patients were 1:1 matched, comprising the well-balanced IBR and AFR groups. Locoregional recurrence-free survival (LRRFS), disease-free survival (DFS), distant metastasis-free survival (DMFS), and breast cancer-specific survival (BCSS) were compared between the matched groups.
Results
After matching, the median follow-up periods were 68 months and 71 months for the IBR and AFR groups, respectively. No significant differences were observed between the IBR and AFR groups regarding the local recurrence (7.2% vs. 5.1%; P = 0.175), regional recurrence (2.1% vs. 1.5%; P = 0.463), or distant metastasis (3.2% vs. 3.2%; P = 1.000) rates. Moreover, no significant difference was observed between the IBR and AFR groups in the LRRFS (hazard ratio, 0.691; 95% CI, 0.433–1.102; P = 0.118), DFS (hazard ratio, 0.709; 95% CI, 0.468–1.076; P = 0.104), DMFS (hazard ratio, 1.006; 95% CI, 0.491–2.059; P = 0.987), or BCSS (hazard ratio, 0.445; 95% CI, 0.111–1.786; P = 0.659).
Conclusion
In this propensity score-matched analysis of oncologic outcomes in patients with primary breast cancer who underwent immediate breast reconstruction with nipple-/skin-sparing mastectomy, no significant differences were observed between the IBR and AFR groups.
Formation of autotriploid Carassius auratus and its fertility-related genes analysis
Authors: Wang Chongqing , Luo Xiang , Qin Huan , Zhao Chun , Yang Li , Yu Tingting , Zhang Yuxin , Huang Xu , Xu Xidan , Qin Qinbo , Liu Shaojun 
Published: April 2021

Abstract
Background

Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR.
Results

Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed genes (DEGs), including 20,155 in F4nRR vs FRCC and 4,107 in M3nRR vs M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs FRCC and M3nRR vs M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR.
Conclusions

Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. Further, RNA-Seq data revealed 8 hub genes related to fertility in 3nRR. We further identified 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish.
Acute Toxicity Assay Using Mysid as an Alternative Test Organism in the Assessment of the Aqueous Fraction of Sediment
Authors: Resgalla Charrid , Silveira Rafaela , Vieira Danielle 
Published: April 2021

Abstract
The evaluation of sediment quality through biological testing of the aqueous fraction can be applied in dredging situations and is associated with a small number of confounding factors. The use of test organisms that are relatively resistant to contaminants is recommended when working with complex mixtures such as sediments. In this study, the sensitivity of the mysid Mysidopsis juniae to total ammonia and metals was investigated in acute toxicity laboratory tests and the results were compared to those obtained in the traditional test with sea urchin embryos and larvae. The ability of the toxicity identification and evaluation (TIE) technique, with the use of EDTA and sodium thiosulfate, to indicate the factors responsible for adverse effects was determined. The results indicate that mysids are almost 5 times more resistant than the sea urchin to unionized ammonia, more sensitive to Cd2+ and Ni2+ and more resistant to Zn2+ and Pb2+. With the TIE technique and with the use of EDTA as a complexing agent, a greater resolution was observed in the diagnosis of the presence of the metals Cd2+ and Ni2+, which could be applied to the aqueous fraction of the sediment.
The role of working alliance in ACT teams: the building blocks retrieved
Authors: Haaren M. van , Jong S. de , Roeg D.P.K. 
Published: April 2021

Abstract
Background. In this study, we aimed to retrieve and operationalize the building blocks of the working alliance in multidisciplinary teams with shared caseloads, for use in daily practice and research to support (Flexible) Assertive Community Treatment teams.Methods. After reviewing literature, concept mapping with professionals and clients was used to define working alliance in (F)ACT. The resulting concept maps formed the basis for the instrument. The instrument was pilot tested with professionals and clients by means of cognitive interviews with a think-aloud procedure.Results. The study led to development of a twenty-five item instrument to assess Working Alliance in Multidisciplinary teams (WAM), comprised of three subscales: bond, task/goal and team, with a version for clients and professionals.Conclusions. The WAM was developed to determine the quality of the working alliance in ACT teams. Future research will focus on testing its psychometric properties and predictive value.
The Institutional Origins of Vaccines Distrust: Evidence from Former-Communist Countries
Authors: Costa-Font Joan , Garcia-Hombrados Jorge , Nicińska Anna 
Published: April 2021

Abstract
How is trust in vaccines affected by exposure to Soviet communism? Using individual level evidence on vaccine trust with regards to its efficiency and safety from a long list of world countries, we document that exposure to Soviet communism reduces trust in vaccination. We show that exposure to socio-political regimes can explain limited trust in vaccines, which is explained by weak trust in government, medical personnel, medical advice from doctors as well as in people from the neighbourhood. These results suggest that roots of vaccine distrust lie in a wider distrust in public and state institutions resulting from the exposure to Soviet communism.
Antibiotic Resistance, Phylogenetic Typing and Virulence Genes Profile Analysis of Uropathogenic Escherichia Coli Isolated From Patients in Southern Iraq
Authors: Allami Mohammed , Bahreini Masoumeh , Sharifmoghadam Mohammad Reza 
Published: April 2021

Abstract
Of the most common infectious diseases that occur mainly by uropathogenic Escherichia coli (UPEC) is urinary tract infections (UTIs). The purpose of this study was to investigate virulence factors, antibiotic resistance, and phylogenetic groups among UPEC strains isolated from patients with UTI in southern Iraq. A total of 100 UPEC isolates were collected from urine samples of UTI patients from various hospitals in southern Iraq, and confirmed by morphological and biochemical tests. Antimicrobial susceptibility testing on isolates was performed by disk diffusion method. Multiplex PCR technique was used to evaluate the phylogenetic groups and the presence of six virulence factor genes; type 1 fimbria (fimH), A-fimbrial adhesion (afa), hemolysin (hly), fimbrial adhesins P (papC), cytotoxic necrosis factor 1 (cnf1), and aerobactin (aer). The majority of isolates belonged to the phylogenetic groups of B2 (55%) and D (32%). The most prevalent virulence factors were fimH (96%), followed by aer (47%), papC (36%), cnf1 (17%), hly (15%), and afa (8%). Phenotypic testing showed that the isolates were most resistant to piperacillin, ticarcillin, amoxicillin/clavulanic acid (92%, 91%, and 88%, respectively) and most sensitive to amikacin and imipenem, respectively. The maximum antibiotic resistance and virulence factors were observed in the phylogenetic group B2. The results showed that the UPEC isolates had all six virulence factors with high frequency and the highest drug resistance. Besides, the results showed a direct relationship between virulence factors, gene diversity, phylogenetic background, and antimicrobial resistance in the UPEC isolates.
A comparison of self-bias measures across cognitive domains
Authors: Amodeo Letizia , Wiersema Jan Roelf , Brass Marcel , Nijhof Annabel 
Published: April 2021

Abstract
Background: The ‘self-bias’ – i.e., the human proneness to preferentially process self-relevant stimuli – is thought to be important for both self-related and social processing. Previous research operationalized the self-bias using different paradigms, assessing the size of the self-bias within a single cognitive domain. Recent studies suggested a reduced self-bias in autism, yet findings are inconsistent. Therefore, the primary goal of the current study was to investigate whether self-biases found across cognitive domains (i.e., perception, memory, attention) are related or independent. The secondary goal was to explore the relationship between these self-biases and the extent of autistic traits in a neurotypical sample.Methods: In an online procedure, 99 Dutch-speaking adults performed three self-processing tasks in counterbalanced order – i.e., the shape-label matching task (perception), the trait adjectives task (memory) and the visual search task (attention) – and completed two self-report measures of ASD symptomatology, i.e., AQ-10 and SRS-A. To control for level of familiarity, self-, close other- and famous other-relevant stimuli were included in each task. Repeated measures ANOVAs were conducted for each task, and both frequentist as well as Bayesian analyses were applied to investigate the correlational patterns between self-bias measures.Results: We observed significant correlations of the self-bias magnitude between memory and attention, as well as attention and perception. However, Bayesian analysis provided only weak support for the latter association. Further, the size of the self-bias was not significantly related across memory and perception. For neither of the tasks, a significant correlation between autistic traits and the self-bias magnitude was found, with Bayesian analyses strongly favoring the null hypothesis.Conclusions: In contrast with the view of a ‘unidimensional’ self-bias, our findings provide evidence for a heterogeneous and multifaceted self consisting of a variety of related and unrelated aspects. None of the self-bias indices were found to relate to autistic traits in our neurotypical sample.
Farmer’s Drought Response and Coping Strategies at Household Level in Southern Tigray, Ethiopia
Authors: Gidey Eskinder , Gebregergis Tirhas , Zeweld Woldegebrial , Gebretsadik Haftamu , Dikinya Ogaile , Mussa Said 
Published: April 2021

Abstract
Background

Drought is one of the most damaging climate-induced threats impacting the lives of many people every year. The purpose of this study was to determine farmer’s drought coping strategies both proactive and reactive responses at household level based on the field survey in Raya Azebo and Raya Chercher districts, southern Tigray, Ethiopia. Agro–climatological based 246 households were sampled from the lowlands (36), midlands (202) and highlands (8). Multinomial logit model was used to identify best drought coping strategies.
Results

about 24.8% of female headed and 75.2% of male headed respondents have experienced mild to extremely severe drought in the last three decades. A significant association between the various drought severity and household heads (chi–square = 9.861, df = 3, p–value < 0.05) observed.
Conclusions

this study concluded that collection and saving of pasture, soil and water conservation practices, and use of weather prediction information to adjust saving and farming system are best proactive drought coping strategies. Whereas, feeding of roasted cactus for livestock, borrowing loans for running small business, selling of household assets and reduction of food consumptions are the major reactive or off–farm drought coping strategies in the study area. If the responses of smallholder farmers are not well supported by the concerned bodies, the existing disaster preparedness and early warning systems in the area might be significantly affected and its impacts will be very serious on both the livelihood of local people and natural resources in the area.
GSDMD-dependent neutrophil extracellular traps formation contributes to fibroblast-like synoviocyte activation in rheumatoid arthritis
Authors: Zhou Kaifeng , Bian Chong , Gu Huijie , Cheng Xiangyang , Huang Zhongyue , Chen Guangnan , Xu Jun , Yin Xiaofan 
Published: April 2021

Abstract
Objective

The activation of NLRP3 inflammasome is critical for rheumatoid arthritis (RA), however, the role of gasdermin D (GSDMD), a newly identified pyroptosis executioner downstream of NLRP3/ASC/Caspase-1, in RA has not been well defined.
Methods

Genetically susceptible mice (DBA/1J) are immunized with a type II bovine collagen emulsion in complete Freund's adjuvant (CFA). Peripheral blood neutrophils were isolated from healthy volunteers and RA patients; cells were treated with serum from RA patients. The activation of GSDMD was analyzed by confocal and western blot. Proinflammatory cytokines in joint of mice were detected by qPCR and ELISA.
Results

The expression of cleaved GSDMD-N terminal in peripheral blood neutrophils from RA patients and the expression of GSDMD-N was positively correlated with the level of IL-1β and IL-18 from RA patients. GSDMD was required for the pathogenesis of RA, and GSDMD inhibition disulfiram significantly suppressed proinflammatory cytokines production and joint damage, leading to reduced arthritis severity score and loss of cartilage in collagen-induced arthritis (CIA) mouse model. Mechanistically, the activation of GSDMD significantly promotes neutrophil extracellular traps (NETs), which are highly immunogenic and decorated with HMGB1 and MMPs. Furthermore, GSDMD-dependent NETs formation facilitated fibroblast-like synoviocytes (FLS) activation and proliferation, driving cartilage and bone destruction, and promoting disease activity in RA.
Conclusions

GSDMD-dependent NETs formation promotes FLS activation during RA pathogenesis.
Extraperitoneal Laparoscopy for Para-Aortic Lymphadenectomy in Endometrial Carcinoma Staging: An Approach with Higher Efficiency
Authors: Zhang Wei , Xia Lingfang , Han Xiaotian , Ju Xingzhu , Wu Xiaohua , Chen Xiaojun 
Published: April 2021

Abstract
Background: To compare the surgical outcomes of extraperitoneal laparoscopic, transperitoneal laparoscopic, and laparotomic para-aortic lymphadenectomy in endometrial carcinoma staging. Methods: We retrospectively reviewed data of all patients diagnosed with primary endometrial carcinoma who were treated at the Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center from 1 January 2017 to 31 December 2019. The numbers of para-aortic lymph nodes, surgical time, complications, blood loss and hospital stay were compared. The patients' medical records and pathological reports were carefully reviewed. Statistical significance was defined as p< 0.05. Results: We retrospectively compared patients who underwent extraperitoneal laparoscopy (Group E, n=20), transperitoneal laparoscopy (Group T, n=21), and laparotomy (Group L, n=135). The median number of para-aortic lymph nodes was significantly higher in Group E than in Group T andL (9.5, 5, and 6, respectively; p=0.004 and 0.0004, respectively). All patients in Group E underwent successfully dissction to the renal vessel level. The median operation time was significantly shorter in Group L than in Groups T and E (94, 174, and 233 minutes, respectively; p<0.0001). The median estimated blood loss volume was higher in Group L than in Groups T and E (200, 100, and 142.5 ml, respectively; all comparisons p<0.001), and the length of hospital stay was significantly longer in Group L than in Groups T and E (6, 56, and 6 days, respectively; all comparisons p<0.001). Conclusion: The extraperitoneal laparoscopic approach for staging endometrial carcinoma harvested higher numbers of para-aortic lymph nodes which could be considered for endometrial carcinoma staging, especially for para-aortic lymph node harvest.
Biosynthesis of Silver Nanoparticles With Antimicrobial and Anticancer Properties Using Two Novel Yeasts
Authors: Liu Xin , Chen Jiale , Yang Wenyu , Qian Yucheng , Pan Jingyu , Zhu Chennianci , Liu Li , Ou Wenbin , Zhao Hongxin , Zhang Dianpeng 
Published: April 2021

Abstract
AgNPs is nanomaterials with many potential biomedical applications. In this study, two novel yeast strains named as HX-YS and LPP-12Y capable of producing biological silver nanoparticles were isolated. Based on sequencing of ribosomal DNA-ITS fragments, as well as partial D1/D2 regions of 26S rDNA be similar with genus Metschnikowia. The BioAgNPs produced by HX-YS and LPP-12Y at pH of 5.0 - 6.0 and 26℃ ranged in size from 50 to 500 nm. The antibacterial activities of yeast BioAgNPs against five pathogenic bacteria were determined. The highest antibacterial effect was observed on P. aeruginosa, with additional obvious effects on E. coli ATCC8099 and S. aureus ATCC10231. Additionally, the BioAgNPs showed antiproliferative effects on lung cancer cell lines H1975 and A579, with low toxicity in Beas 2B normal lung cells. Therefore, the AgNPs biosynthesized by HX-YS and LPP-12Y may have potential applications in the treatment of bacterial infections and cancer.
Gas Embolism Assessment in Sea Turtles: Predictors of Mortality for the Deadliest Pathology related to Trawling
Authors: Franchini Delia , Valastro Carmela , Ciccarelli Stefano , Trerotoli Paolo , Paci Serena , Caprio Francesco , Lucchetti Alessandro , Bello Antonio Di 
Published: April 2021

Abstract
Sea turtles that are entrapped in static and towed nets may develop gas embolism which can lead to severe organ injury and death. Physical and physiologic factors associated with gas-embolism and predictors of mortality were analysed from 482 bycaught loggerheads. We found 204 turtles affected by gas-embolism and significant positive correlations between the presence of gas-embolism and duration, depth of trawl and turtle size and temperature and between mortality and neurological deficits, significant acidosis and involvement of > 12 cardiovascular sites and the left atrium and sinus venosus-right atrium. 164 turtles were released to the sea. Results of this study may be useful in clinical evaluation, prognostication, and treatment for turtles affected by gas-embolism, but bycatch reduction must become a priority for major international organizations.
Impact of anemia on in-stent restenosis after percutaneous coronary intervention
Authors: Hu Huilin , Wang Shijun , Tang Guanmin , Zhai Changlin , Shen Liang 
Published: April 2021

Abstract
Background Anemia is a well-recognized risk factor for adverse events after percutaneous coronary intervention (PCI), but data regarding the association between anemia and in-stent restenosis (ISR) remain limited.Methods A total of 538 patients who underwent PCI between January 2018 and September 2019 and performed follow-up angiography 9–12 months after the initial PCI was enrolled in the study. Baseline clinical and procedural characteristics were compared between ISR and non-ISR group. Multivariate logistic regression analysis was employed to determine the independent predictors of ISR.Results The incidence of anemia in patients with ISR was 53.5% and 19.0% in the non-ISR group, which was significantly different (P ༜0.001). The rate of diabetes, chronic kidney disease (CKD), bifurcation lesion and calcification was significantly higher in ISR group. In addition, low-density lipoprotein cholesterol (LDL-c), multiple stenting and stent diameter were also significantly related with ISR. After multivariate logistic analysis, anemia (odds ratio [OR], 2.786; 95% confidence interval [CI], 1.091 to 7.115; P = 0.032) together with LDL-c (OR, 1.682; 95% CI, 1.145 to 2.469; P = 0.008), diabetes (OR, 3.582; 95% CI, 1.406 to 9.125; P = 0.007), CKD (OR, 2.841; 95% CI, 1.006 to 8.027; P = 0.049), multiple stenting (OR, 2.823; 95% CI, 1.184 to 6.731; P = 0.019), and stent diameter (OR, 2.778; 95% CI, 1.069 to 7.194; P = 0.036) were closely associated with ISR.Conclusion Anemia is closely associated with ISR after PCI, patients with lower hemoglobin have a higher risk of ISR.
Factors related to myopia progression in orthokeratology practice: A 2-year follow-up study
Authors: Wen Shijin , Ma Siqi , Xiao Chuchu , Hu Shengfa , Ran Xufang , Tan Xingping , Xia Xiaobo , Zhou Xia 
Published: April 2021

Abstract
The incidence of myopia in adolescents is gradually increasing, and orthokeratology has achieved effective effects in controlling the progress of myopia, but the effects are mixed. The retrospective study included 30 monocular orthokeratology (ortho-k) lens-treated adolescents to explore the true effectiveness of ortho-k lenses and 36 binocular ortho-k lenses-treated adolescents to study the influencing factors of ortho-k lenses. After 12 months, among 30 adolescents treated with monocular ortho-k lenses, the average axial elongation in the ortho-k group was significantly less than that in the control group (P = 0.002). After 24 months, among 36 adolescents treated binocular ortho-k lenses, the axial elongation in the different initial age groups and different initial myopia groups were significantly different (all P < 0.05). Axial elongation correlated negatively with initial myopia during follow-up periods. In adolescents with myopia, axial elongation can be controlled effectively using an ortho-k lens. Younger children with initial higher myopia will benefit more than older children with initial lower myopia.