bluuebunny/crossref_metadata_2025_split huggingface dataset:
PPEs From Nigerian Academia: Flattening The COVID-19 Curve With 3D Printing And Locally Sourced Intervention
Authors: Folarin Royhaan , Chagas Andre , Bukar Mahmoud , Akinbo Abisola , Runsewe-Abiodun Tamra , Ogundahunsi Omobola , Adedeji Ahmed 
Published: July 2021

Abstract
Background:Personal protective equipment (PPE) supply shortages were a big issue in the fight against COVID-19 around the world at the onset of the pandemic, requiring all communities to find innovative ways to make and supply PPE for health workers, vulnerable people and the general public. To this end and in line with the WHO’s call for 40% raise in PPE production across the world, we sought to alleviate the PPE shortage and to support our local communities using 3D printing (additive manufacturing) and free and open-source hardware (FOSH). Methods:Utilising a 3D printer and off-the-shelf components, reusable face shields and face masks were produced based on open source designs. Off-the-shelf components included transparent polyvinyl chloride (PVC) visor, ribbon tapings and elastic bands for the face shields, as well as cushioning pads (clothed foams) and filters (sensitive tissues) for the face masks. Hygienic measures employed during fabrication included the assembly following safety protocols, disinfection of products with ultraviolet bactericidal lamps, ensuring hygiene during collection and distribution. Users’ real-life experience and feedback were utilised to modify and improve on quality and adaptability of the designs.Results:In a period of three months, over 400 reusable 3D-printed face shields and face masks were produced by a team of academics, for health practitioners, other professionals and people across the Olabisi Onabanjo University community and other cities within Ogun and Lagos states. Conclusions:More awareness is generally required on the potentials of 3D printing and FOSH in the global south, particularly in universities and research institutions where innovative alternatives to expensive equipment remain vital. Our feat corroborated and advocated these potentials in a low-income setting like Nigeria, where the immediate response and synergy between academics, and researchers yielded a substantial number of PPE to front line workers, in a timely manner at the peak of the viral transmission and lockdowns- a period wherein manufacturers of PPEs struggled to establish their commercial logistics. We emphasize the need for university managements to support academics and researchers strongly to deliver on much needed community support in crises time, and encourage governmental and non-governmental bodies to consider investing in this innovative self-reliant perspective through their research funding and managemnt programmes in a bid to achieving a lot more with the less funds.Trial registration:Not applicable.
Predicting sleep quality from brain white matter volume: A voxel-based morphometry study
Authors: Bai Youling , Zhang Li , Liu Chengwei , Cui Xiaobing , Dan Li , Yin Huazhan 
Published: July 2021

Abstract
Most previous studies have explored the relationship between gray matter volume and sleep quality, but little is known about the relationship between white matter volume and sleep quality. Data were collected using the Pittsburgh Sleep Quality Index and voxel-based morphometry among 352 healthy college students. Results showed that the global PSQI score was negatively associated with the white matter volume, including in the right middle occipital gyrus, the left superior temporal gyrus, the right the precentral gyrus, the left supramarginal gyrus, the left middle frontal gyrus, the left precunes, and the right superior frontal gyrus. Results also indicated that the white matter volume in specific regions negatively predicted the factor of PSQI. These specific brain regions may be replicated in brain areas related to sleep quality. In summary, we suggested that an investigation of white matter structural alterations in the specific regions might be beneficial to tackle underlying neurological mechanisms of sleep quality.
E-Commerce Combined with Enterprise Management using Cloud Computing for Business Sector
Authors: Qi Xiaotian , Joghee Shanmugan , Mohammed Abdul Salam 
Published: July 2021

Abstract
In the techno world of today, with the rapid advancement of new economic construction, a diversity of information expertise is emerging. Through implementing the advanced cloud computing business model, enterprises have resolved the problems with the shortage of capital, workforce, and technology, which are vital in e-commerce operations to handle the conflict with the e-commerce undertakings of enterprises and the scarcity of resources. Cloud computing can play a critical role in increasing business processes through e-commerce applications, leading to higher financial value for enterprises. Hence, in this paper, Cloud Computing assisted Enterprise Resource Planning (CCAERP) has been proposed to base on an e-commerce application for enterprise management. Currently, most internal e-commerce systems and ERP systems are relatively autonomous. Nevertheless, a single method of operating proved capable of satisfying the requirements of business growth with growing fierce market competition. Effective enterprise resource planning and e-commerce convergence have been one of the subjects of business growth in the new era. This paper first analyzes the relationship between Enterprise Resource Planning and e-commerce and then examines and explores the incorporation requirements and feasibility of the enterprise.
Genetic Mapping of Adult-Plant Resistance Genes to Powdery Mildew in Triticale
Authors: Dyda Mateusz Maksymilian , Tyrka Mirosław , Gołębiowska Gabriela , Rapacz Marcin , Wędzony Maria 
Published: July 2021

Abstract
Triticale is a cereal of high economic importance, however along with the increase in the area of this cereal, it is more often infected by the fungal pathogen Blumeria graminis, which causes powdery mildew. The rapid development of molecular biology techniques, in particular methods based on molecular markers may be an important tool used in modern plant breeding. Development of genetic maps, location of the QTLs defining the region of the genome associated with resistance and selection of markers linked to particular trait can be used to select resistant genotypes as well as to pyramidize several resistance genes in one variety. In this paper we present a new, high-density genetic map of triticale doubled haploids (DH) population ‘Grenado’ x ‘Zorro’ composed of DArT, silicoDArT and SNP markers. Composite interval mapping method was used to detect eight QTL regions associated with the area under disease progress curve (AUDPC) and 15 regions with the average value of powdery mildew infection (avPM) based on observation conducted in 3-year period in three different locations across the Poland. Two regions on rye chromosome 4R, and single loci on 5R and 6R were reported for the first time as regions associated with powdery mildew resistance. Among all QTLs, 14 candidate genes were identified coded cyclin-dependent kinase, serine/threonine-protein kinase-like protein as well as AMEIOTIC 1 homolog DYAD-like protein, DETOXIFICATION 16-like protein and putative disease resistance protein RGA3. Three of identified candidate genes were found among newly described QTL regions associated with powdery mildew resistance in triticale.
Role of Acquired Von Willebrand Syndrome in Patients Treated Using Impella RP® Devices: A Retrospective Cohort Study
Authors: Oezkur Mehmet , Reda Sara , Rühl Heiko , Theuerkauf Nils , Kreyer Stefan , Duerr Daniel , Charitos Efstratios , Silaschi Miriam , Medina Marta , Zimmer Sebastian , Putensen Christian , Treede Hendrik 
Published: July 2021

Abstract
Background: Axial flow pumps are standard treatment in cases of cardiogenic shock and high-risk interventions in cardiology and cardiac surgery, although the optimal anticoagulation strategy remains unclear. We evaluated whether laboratory findings could predict bleeding complications and acquired von Willebrand syndrome (avWS) among patients who were treated using axial flow pumps.Methods and Results: We retrospectively evaluated 60 consecutive patients who received Impella® devices (Impella® RP: n=20, Impella® CP/5.0: n=40; Abiomed Inc., Danvers, USA) between January 2019 and December 2020. Thirty-two patients (53.3%) experienced major or fatal bleeding complications (Bleeding Academic Research Consortium score of >3) despite intravenous heparin being used to maintain normal activated partial thromboplastin times (40–50 s). Extensive testing was performed for 28 patients with bleeding complications (87.5%). Relative to patients with left ventricular support, patients with right ventricular support were less likely to develop avWS (87.5% vs. 58.8%, p=0.035). Bleeding was significantly associated with avWS (odds ratio [OR]: 20.8, 95% confidence interval [CI]: 3.3–128.5; p=0.001) and treatment duration (OR: 1.3, 95% CI: 1.09–1.55; p=0.003). Patients with avWS had longer Impella® treatment than patients without avWS (2 days [1–4.7 days] vs. 7.3 days [3.2–13.0 days]). Conclusions: Anticoagulation monitoring based on aPTT was not sufficient to prevent bleeding complications during axial flow pump support. A more targeted anticoagulation monitoring is needed for patients who receive Impella® devices.
A Comparative Study of Water Quality and Trophic State Contribution on Natural and Artificial Lakes Global Warming Potential
Authors: Samiotis Georgios , Mavromatidou Charoula , Ziagova Maria , Trikilidou Eleni , Amanatidou Elisavet 
Published: July 2021

Abstract
Natural lakes and reservoirs are emitters of GHGs in the atmosphere contributing to 31% of the annual CO2 emissions of those from fossil fuel combustion. Measurements of GHGs emissions in reservoirs demonstrate that hydropower may actually not be as “green” as once thought. It is estimated that emissions from reservoirs may be equivalent to 7% of the global warming potential (GWP) of other documented anthropogenic emissions. Aim of this work is to assess the impact of water quality deterioration and the subsequent increment of biological productivity of a waterbody on GHGs emissions. Therefore, the trophic state, the carbonic GHGs emissions and the GWP of one natural lake domestic wastewater receiver and two different age hydroelectric reservoirs, located in North West Greece, were studied. Gross emissions of CO2 and CH4 were in-situ measured using a static floating chamber and specific emissions as well as GWP were calculated. Furthermore, water quality and trophic state were evaluated based on the application of ΥΔΩΡ (hydõr) Water Quality Index and Florida Trophic State Index using physicochemical characteristics measurements. Data statistical interpretation revealed that CH4 has strong positive correlation with GWP, temperature, water quality and trophic state. There is a seasonal variation of GWP that follows the seasonal variation of CH4 emissions induced by water temperature. Specific CH4 emission rate presents the most reliable indicator for assessing the impact of a waterbody in terms of GWP, especially of a hypertrophic one. Water quality and trophic state indices can be used for a rough comparison of GWP between waterbodies with the same climatic conditions.
Zinc Complex of 3,5-di-tert-butyl Salicylate Inhibits the Proliferation, Migration, and Invasion of Triple-Negative Breast Cancer Cells
Authors: Chen Heng , Wang Dong , Fan Limei , Zhang Weiran , Xu Jinhua , Liu Yunyi 
Published: July 2021

Abstract
Zinc complex of 3,5-di-tert-butyl salicylate (Zn{[CH3)3C]2Sal}22-) is a zinc ion chelate of salicylate. In this study, we found that it inhibits the proliferation, invasion, migration and induce apoptosis of triple-negative breast cancer 4T1 cells. The results of RNA-seq showed that expression of 17 genes was up-regulated and 26 genes was down-regulated significantly by Zn{[CH3)3C]2Sal}22- treatment. Further GO analysis and KEGG analysis showed that the activity of Zn{[CH3)3C]2Sal}22- against triple-negative breast cancer cells may be involved JAK-STAT3, HIF-1, and TNF signaling pathways. The expression of key genes was verified by RT-PCR. The phosphorylation of STAT3 and its upstream SRC decreased drastically upon Zn{[CH3)3C]2Sal}22- treatment as analyzed by western blot. Our results indicate that Zn{[CH3)3C]2Sal}22- inhibits the activity of TNBC cells by down-regulating STAT3 signaling pathway.
Adrenal Insufficiency in Patients with COVID-19
Authors: de Almeida Carlos Augusto Pereira , Guimarães Marilia Rodovalho , de Oliveira Márcia Fernanda Arantes , Seabra Victor , Smolentzov Igor , Reichert Bernardo Vergara , Lins Paulo Ricardo Gessolo , Rodrigues Camila Eleutério , Andrade Lucia Conceição 
Published: July 2021

Abstract
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which can result in coronavirus disease 2019 (COVID-19), has caused an unprecedented global pandemic. The epicenter of that pandemic in Latin America was Brazil, where more than 500,000 deaths have been attributed to COVID-19.
Genetic Dissection of Drought Resistance of Common Bean at the Seedling Stage by Genome-wide Association Study
Authors: Wu Lei , Chang Yujie , Wang Lanfen , Wang Shumin , Wu Jing 
Published: July 2021

Abstract
A variety of adverse conditions, including drought stress, severely affect common bean production. Molecular breeding for drought resistance has been proposed as an effective and practical way to improve the drought resistance of common bean. A genome-wide association analysis was conducted to identify drought-related loci based on survival rates at the seedling stage using a natural population consisting of 400 common bean accessions and 3832340 SNPs. The coefficient of variation ranged from 40.90% to 56.22% for survival rates in three independent experiments. A total of 12 associated loci containing 89 significant SNPs were identified for survival rates at the seedling stage. Four loci overlapped in the region of the QTLs reported to be associated with drought resistance. According to the expression profiles, gene annotations and references of the functions of homologous genes in Arabidopsis, 39 genes were considered potential candidate genes selected from 199 genes annotated within all associated loci. A stable locus (Locus_10) was identified on chromosome 11, which contained LEA, aquaporin, and proline-rich protein genes. We further confirmed the drought-related function of an aquaporin (PvXIP1;2) located at Locus_10 by expression pattern analysis, phenotypic analysis of PvXIP1;2-overexpressing Arabidopsis and Agrobacterium rhizogenes-mediated hairy root transformation systems, indicating that the association results can facilitate the efficient identification of genes related to drought resistance. These loci and their candidate genes provide a foundation for crop improvement via breeding for drought resistance in common bean.
Investigating the impact of COVID-19 on performance and image enhancing drug use
Authors: Dunn Matthew , Piatkowski Timothy 
Published: July 2021

Abstract
Background
Emerging research has suggested that the COVID-19 pandemic has had some impact on substance use patterns. The aim of the study was to conduct a rapid survey to assess the impact of COVID-19 on performance and image enhancing drug (PIED) use and training, and any subsequent negative physical or mental health outcomes.
Methods
During 2020, a convenience sample of 60 PIED consumers (mean age = 26.69; 68.3% located outside Australia) completed a quantitative anonymous online survey exploring how the coronavirus pandemic impacted patterns of PIED use and associated exercise habits. The survey was administered via the Qualtrics platform and distributed online through PIED forums as well as through the investigator's networks. Participants were asked about their PIED use and exercise habits prior to and during restricted movement ‘lockdowns’.
Results
Pre-COVID, the majority of the sample opted to ‘blast-cruise’ (an initial high dose, followed by a lower maintenance dose; 71.7%, n = 43). During lockdown, (45%, n = 27) reported a change in PIED use as a result of the restrictions. In light of health concerns during COVID-19, a majority of men (60%, n = 36) did not take any extra precautions relating to their PIED use. A subgroup of men ceased using PIEDs completely (16.7%, n = 10) with the majority (80%, n = 8) of that subgroup following post-cycle therapy (PCT) of some kind.
Conclusions
This study contributes to the emerging literature of the impact of the COVID-19 pandemic on substance use, specifically PIED use among men. The results suggest that the pandemic did influence the choice of PIEDs that participants consumed, although there was little disruption to patterns of exercise, an important aspect of PIED use. Of the men who did cease use completely, the majority reported little issue with PCT access; those who reported difficulty accessing PCT compounds indicated experience some mental health concerns related to ceasing their PIED use. Clinicians and those who come into contact with this group should be alert for any negative physical or mental health concerns resulting from disrupted or ceased PIED use.
Attempt to Describe Phase Slips by Means of an Adiabatic Approximation
Authors: Berger Jorge 
Published: July 2021

Abstract
As a plausibility test for the feasibility of extension of the quasiclassical Keldysh–Usadel technique to slowly varying situations, we assess the influence of the time-derivative term in the time-dependent Ginzburg–Landau equation. We consider cases in which the superconducting state in a nanowire varies slowly, either because the voltage applied on it is small, or because most of phase drift takes place next to the boundaries. An approximation without this time derivative can describe the superconducting state away from phase slips, but is unable to predict the value or the existence of a critical voltage at which evolution becomes non-stationary.
Description of Paenibacillus albicerus sp. nov. and Niallia alba sp. nov., isolated from digestive syrup.
Authors: Thorat Vipool , Kirdat Kiran , Tiwarekar Bhavesh , Dhanavade Priyanka , Karodi Prachi , Shouche Yogesh , Sathe Shivaji , Lodha Tushar , Yadav Amit 
Published: July 2021

Abstract
Two aerobic, Gram-stain variable, catalase and oxidase-positive, spore-forming, motile rods of strain UniB2T and UniB3T, were isolated from digestive syrup containing fungal diastase (10 mg/ml), pepsin (2 mg/ml) and sugar base containing polyethene glycol. Based on 16S rRNA gene sequence analysis, strain UniB2T has the highest sequence similarity with Paenibacillus humicus NBRC 102415T (98.30 %) and strain UniB3T showed the highest sequence similarity with Niallia circulans DSM 11T (98.95 %). The DNA G+C content of UniB2T was 63.74 mol %. The dDDH and ANI values between the strain UniB2T and its phylogenetically close relative were <38.3 % and <89.55 %, respectively. The major fatty acids of the strain UniB2T were C16:00 (13.9 %), C15:0 anteiso (39.7 %), C17:0 anteiso (15.5 %). The DNA G+C content of UniB3T was 35.6 mol%. The dDDH and ANI values between the strain UniB3T and its close relatives were <29.1 % and 84.62%, respectively. The major fatty acids of strain UniB3T were C16:0 (13.54 %), C15:0 anteiso (40.09 %) and C17:0 anteiso (16.03 %). Major polar lipids for both strains were Diphosphotidylglycerol and Phosphatidylethanolamine. Additionally, both the strains showed unique carbon utilization and assimilation pattern that differentiated them from their phylogenetically related neighbours. These phenotypic, genotypic and chemotaxonomic characters indicated the strains UniB2T and UniB3T represent two novel species for which the names Paenibacillus albicerus sp. nov. (Type strain UniB2T =MCC 3997T =KCTC 43095T =JCM34513T) and Niallia alba sp. nov. (Type strain UniB3T = MCC 3998T =KCTC 43235T =JCM 34492T) are proposed.
Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Authors: Bouwknegt Jonna , Koster Charlotte C. , Vos Aurin M. , Ortiz-Merino Raúl A. , Wassink Mats , Luttik Marijke A. H. , Broek Marcel van den , Hagedoorn Peter L. , Pronk Jack T. 
Published: July 2021

Abstract
Background
In most fungi, quinone-dependent Class-II dihydroorotate dehydrogenases (DHODs) are essential for pyrimidine biosynthesis. Coupling of these Class-II DHODHs to mitochondrial respiration makes their in vivo activity dependent on oxygen availability. Saccharomyces cerevisiae and closely related yeast species harbor a cytosolic Class-I DHOD (Ura1) that uses fumarate as electron acceptor and thereby enables anaerobic pyrimidine synthesis. Here, we investigate DHODs from three fungi (the Neocallimastigomycete Anaeromyces robustus and the yeasts Schizosaccharomyces japonicus and Dekkera bruxellensis) that can grow anaerobically but, based on genome analysis, only harbor a Class-II DHOD.
Results
Heterologous expression of putative Class-II DHOD-encoding genes from fungi capable of anaerobic, pyrimidine-prototrophic growth (Arura9, SjURA9, DbURA9) in an S. cerevisiae ura1Δ strain supported aerobic as well as anaerobic pyrimidine prototrophy. GFP-tagged SjUra9 and DbUra9 were localized to S. cerevisiae mitochondria, while ArUra9, whose sequence lacked a mitochondrial targeting sequence, was localized to the yeast cytosol. Experiments with cell extracts showed that ArUra9 used free FAD and FMN as electron acceptors. A strain expressing DbURA9 initially grew slowly without pyrimidine supplementation. Adapted faster growing DbURA9-expressing strains showed mutations in FUM1, which encodes fumarase. Expression of SjaUra9 in S. cerevisiae reproducibly led to loss of respiratory competence and mitochondrial DNA, which coincided with the natural respiratory deficiency of Sch. japonicus. A cysteine residue (C265 in SjUra9) in the active sites of all three anaerobically active Ura9 orthologs was shown to be essential for anaerobic activity of SjUra9 but not of ArUra9.
Conclusions
Activity of fungal Class-II DHODs was long thought to be dependent on an active respiratory chain, which in most fungi requires the presence of oxygen. By heterologous expression experiments in S. cerevisiae, this study shows that phylogenetically distant fungi independently evolved Class-II dihydroorotate dehydrogenases that enable anaerobic pyrimidine biosynthesis. Further structure-function studies are required to understand the mechanistic basis for the anaerobic activity of Class-II DHODs and an observed loss of respiratory competence in S. cerevisiae strains expressing an anaerobically active DHOD from Sch. japonicus.