bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sulodexide reduces glucose induced senescence in human retinal endothelial cells
Authors: Gericke A. , Suminska-Jasińska K. , Bręborowicz A. 
Published: June 2021

AbstractChronic exposure of retinal endothelium cells to hyperglycemia is the leading cause of diabetic retinopathy. We evaluated the effect of high glucose concentration on senescence in human retinal endothelial cells (HREC) and modulation of that effect by Sulodexide. Experiments were performed on HREC undergoing in vitro replicative senescence in standard medium or medium supplemented with glucose 20 mmol/L (GLU) or mannitol 20 mnol/L (MAN). Effect of Sulodexide 0.5 LRU/mL (SUL) on the process of HREC senescence was studied. Glucose 20 mmol/L accelerates senescence of HREC: population doubling time (+ 58%, p < 0.001) β-galactosidase activity (+ 60%, p < 0.002) intracellular oxidative stress (+ 65%, p < 0.01), expression of p53 gene (+ 118%, p < 0.001). Senescent HREC had also reduced transendothelial electrical resistance (TEER) (− 30%, p < 0.001). Mannitol 20 mmol/L used in the same scenario as glucose did not induce HREC senescence. In HREC exposed to GLU and SUL, the senescent changes were smaller. HREC, which became senescent in the presence of GLU, demonstrated higher expression of genes regulating the synthesis of Il6 and VEGF-A, which was reflected by increased secretion of these cytokines (IL6 + 125%, p < 0.001 vs control and VEGF-A + 124% p < 0.001 vs control). These effects were smaller in the presence of SUL, and additionally, an increase of TEER in the senescent HREC was observed. Chronic exposure of HREC to high glucose concentration in medium accelerates their senescence, and that process is reduced when the cells are simultaneously exposed to Sulodexide. Additionally, Sulodexide decreases the secretion of IL6 and VEGF-A from senescent HREC and increases their TEER.
Expression of barrier proteins in the skin lesions and inflammatory cytokines in peripheral blood mononuclear cells of atopic dogs
Authors: Kanwal Sarita , Singh Shanker K. , Soman Sandeep P. , Choudhury Soumen , Kumari Priyambada , Ram Pradeep K. , Garg Satish K. 
Published: June 2021

AbstractAtopic dermatitis (AD) is one of the most common skin diseases of dogs. Defects in the skin barrier and overproduction of inflammatory cytokines may be the pathogenesis of canine AD. Therefore, the present study was aimed to quantify the gene expression of certain skin barrier proteins and inflammatory cytokines in dogs with AD. Eleven dogs with AD and three healthy dogs were included in the present study. The skin barrier proteins, namely Filaggrin (FLG) and Involucrin (IVL), gene expression was quantified by Real-time PCR in the lesional skin tissues of the atopic dogs and normal skin of the healthy dogs. In addition to the skin proteins, the gene expressions of the interleukin (IL)-13, IL-31, and tumour necrosis factor (TNF)-α were also quantified in the peripheral blood mononuclear cells (PBMCs) of these dogs. Compared to the healthy dogs, significantly higher (P ≤ 0.01) FLG gene expression and significantly (P ≤ 0.05) lower expression of the IVL gene were quantified in the skin of atopic dogs. Further, the dogs with AD revealed significantly higher expression of TNF-α (P ≤ 0.01), IL-31 (P ≤ 0.05), and IL-13 (P ≤ 0.05) as compared to the healthy dogs. The findings of our present study evidently suggest significantly increased and decreased expressions of FLG and IVL genes, respectively, which may be responsible for disruption of the skin barrier in dogs with AD. While, the over-expressions of TNF-α, IL-31, and IL-13 genes might be attributed to the clinical pathology and manifestations of AD in dogs. However, further studies are warranted to substantiate our hypothesis about pathogenesis and clinical manifestation of AD in dogs by including a large number of animals.
Impacts of sunspot number and Geomagnetic aa-index on climate of Wet Zone West Africa during solar cycles 22–24
Authors: Hanson Esther A. , Okeke Francisca N. 
Published: June 2021

AbstractUsing the facilities at Heliophysics Science Division of NASA Goddard Space Flight Center, Greenbelt, MD, USA, we attempted to investigate the impact of solar magnetic activities on the climate of Wet Zone West Africa. The solar activity data namely, Sunspot Number (SSN) was obtained from the Royal Observatory of Belgium, Brussels; and Geomagnetic aa-index was obtained from World Data Center, Kyoto, Japan. Surface Air Temperature (SAT) and Rainfall data [for Port Harcourt in Nigeria and Abidjan in Cote D’Ivoire] were obtained from the HadCRUT-4 project of Climate Research Unit of University of East Anglia, United Kingdom. Firstly, we carried out Time Series Analysis of SSN and Geomagnetic aa-index spanning from 1950 to 2016. Secondly, we performed Regression Analysis on both solar activity data and climate variables to estimate the impact of solar magnetic activity on the Wet Zone West African climate. The Time Series Analysis showed that SSN variation was in-phase with Geomagnetic aa-index in all the solar cycles studied. Thus, Geomagnetic aa-index can be used as a proxy for studying solar magnetic activities. Performance of Regression Analysis showed that SSN regressed on SAT and Rainfall amounted to an average of 0.49 and 0.02% respectively throughout Solar Cycles 22–24. Furthermore, a regression of Geomagnetic aa-index on SAT and Rainfall yielded an average of 0.145 and 0.125% respectively. Our models showed that the variability of SAT and Rainfall in Wet Zone West Africa during Solar Cycles 22–24 are far less than 1%. Hence, the influence of SSN and Geomagnetic aa-index on SAT and Rainfall is less than 1%; and could cause ‘very small’ effect. These weak impacts are proofs that the variability of SAT and Rainfall were most probably not effected by SSN and Geomagnetic aa-index. Consequently, the variability of SAT and Rainfall in Wet Zone West Africa could not be attributed to SSN and Geomagnetic aa-index. We therefore, attempt to conclude that climate variability in Wet Zone West Africa is most probably not driven by solar magnetic activity, but could be attributed to anthropogenic activities.
Late Miocene megalake regressions in Eurasia
Authors: Palcu Dan Valentin , Patina Irina Stanislavovna , Șandric Ionuț , Lazarev Sergei , Vasiliev Iuliana , Stoica Marius , Krijgsman Wout 
Published: June 2021

AbstractThe largest megalake in the geological record formed in Eurasia during the late Miocene, when the epicontinental Paratethys Sea became tectonically-trapped and disconnected from the global ocean. The megalake was characterized by several episodes of hydrological instability and partial desiccation, but the chronology, magnitude and impacts of these paleoenvironmental crises are poorly known. Our integrated stratigraphic study shows that the main desiccation episodes occurred between 9.75 and 7.65 million years ago. We identify four major regressions that correlate with aridification events, vegetation changes and faunal turnovers in large parts of Europe. Our paleogeographic reconstructions reveal that the Paratethys was profoundly transformed during regression episodes, losing ~ 1/3 of the water volume and ~ 70% of its surface during the most extreme events. The remaining water was stored in a central salt-lake and peripheral desalinated basins while vast regions (up to 1.75 million km2) became emergent land, suitable for development of forest-steppe landscapes. The partial megalake desiccations match with climate, food-web and landscape changes throughout Eurasia, although the exact triggers and mechanisms remain to be resolved.
Gravitational effects of scene information in object localization
Authors: Kosovicheva Anna , Bex Peter J. 
Published: June 2021

AbstractWe effortlessly interact with objects in our environment, but how do we know where something is? An object’s apparent position does not simply correspond to its retinotopic location but is influenced by its surrounding context. In the natural environment, this context is highly complex, and little is known about how visual information in a scene influences the apparent location of the objects within it. We measured the influence of local image statistics (luminance, edges, object boundaries, and saliency) on the reported location of a brief target superimposed on images of natural scenes. For each image statistic, we calculated the difference between the image value at the physical center of the target and the value at its reported center, using observers’ cursor responses, and averaged the resulting values across all trials. To isolate image-specific effects, difference scores were compared to a randomly-permuted null distribution that accounted for any response biases. The observed difference scores indicated that responses were significantly biased toward darker regions, luminance edges, object boundaries, and areas of high saliency, with relatively low shared variance among these measures. In addition, we show that the same image statistics were associated with observers’ saccade errors, despite large differences in response time, and that some effects persisted when high-level scene processing was disrupted by 180° rotations and color negatives of the originals. Together, these results provide evidence for landmark effects within natural images, in which feature location reports are pulled toward low- and high-level informative content in the scene.
Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2–STAT3 pathway
Authors: Yang Shujun , Song Yaodong , Wang Qiaofang , Liu Yanna , Wu Zhongwei , Duan Xiaojia , Zhang Yan , Bi Xiuqian , Geng Yuanzhang , Chen Sanyang , Zhu Changju 
Published: June 2021

AbstractSevere acute pancreatitis (SAP) is often associated with pulmonary inflammation leading to acute lung injury. Daphnetin, a natural coumarin derivative, has been reported to exert anti-inflammatory effects. Here, we explored the effect and possible mechanism of daphnetin in a mouse model of SAP-associated lung injury induced by an intraperitoneal injection of l-arginine. The severity of pancreatic and lung injury is determined by histology and its score. Immunostaining of inflammatory and apoptotic cells was used to demonstrate lung tissue inflammation and apoptosis; ELISA analysis of serum and tissue cytokine levels; and western blotting and immunohistochemical staining for the activated Janus kinase 2 (JAK2)–signal transducer and activator of transcription protein 3 (STAT3) signalling pathway in lung tissues. Daphnetin pretreatment significantly reduced SAP-induced pancreatic and lung tissue damage, reduced interleukin-6 and tumour necrosis factor-α concentrations in both serum and lung tissues, reduced serum amylase and myeloperoxidase activities, and reduced macrophage (CD11b) and neutrophil (Ly6G) infiltration and cell apoptosis in the lung tissue. Moreover, SAP-induced phosphorylation of JAK2 and STAT3 in the lung tissue was also significantly diminished by the daphnetin pretreatment. These results indicated that daphnetin reduces SAP-associated lung tissue damage, likely by inhibiting the activation of JAK2–STAT3 signalling.
Fraud in highly appreciated fish detected from DNA in Europe may undermine the Development Goal of sustainable fishing in Africa
Authors: Blanco-Fernandez Carmen , Ardura Alba , Masiá Paula , Rodriguez Noemi , Voces Laura , Fernandez-Raigoso Marcelino , Roca Agustín , Machado-Schiaffino Gonzalo , Dopico Eduardo , Garcia-Vazquez Eva 
Published: June 2021

AbstractDespite high effort for food traceability to ensure safe and sustainable consumption, mislabeling persists on seafood markets. Determining what drives deliberate fraud is necessary to improve food authenticity and sustainability. In this study, the relationship between consumer’s appreciation and fraudulent mislabeling was assessed through a combination of a survey on consumer’s preferences (N = 1608) and molecular tools applied to fish samples commercialized by European companies. We analyzed 401 samples of fish highly consumed in Europe and worldwide (i.e. tuna, hake, anchovy, and blue whiting) through PCR-amplification and sequencing of a suite of DNA markers. Results revealed low mislabeling rate (1.9%), with a higher mislabeling risk in non-recognizable products and significant mediation of fish price between consumer´s appreciation and mislabeling risk of a species. Furthermore, the use of endangered species (e.g. Thunnus thynnus), tuna juveniles for anchovy, and still not regulated Merluccius polli hake as substitutes, points towards illegal, unreported and/or unregulated fishing from African waters. These findings reveal a worrying intentional fraud that hampers the goal of sustainable seafood production and consumption, and suggest to prioritize control efforts on highly appreciated species.
Increased extravascular lung water index (EVLWI) reflects rapid non-cardiogenic oedema and mortality in COVID-19 associated ARDS
Authors: Rasch Sebastian , Schmidle Paul , Sancak Sengül , Herner Alexander , Huberle Christina , Schulz Dominik , Mayr Ulrich , Schneider Jochen , Spinner Christoph D. , Geisler Fabian , Schmid Roland M. , Lahmer Tobias , Huber Wolfgang 
Published: June 2021

AbstractNearly 5% of patients suffering from COVID-19 develop acute respiratory distress syndrome (ARDS). Extravascular lung water index (EVLWI) is a marker of pulmonary oedema which is associated with mortality in ARDS. In this study, we evaluate whether EVLWI is higher in patients with COVID-19 associated ARDS as compared to COVID-19 negative, ventilated patients with ARDS and whether EVLWI has the potential to monitor disease progression. EVLWI and cardiac function were monitored by transpulmonary thermodilution in 25 patients with COVID-19 ARDS subsequent to intubation and compared to a control group of 49 non-COVID-19 ARDS patients. At intubation, EVLWI was noticeably elevated and significantly higher in COVID-19 patients than in the control group (17 (11–38) vs. 11 (6–26) mL/kg; p < 0.001). High pulmonary vascular permeability index values (2.9 (1.0–5.2) versus 1.9 (1.0–5.2); p = 0.003) suggested a non-cardiogenic pulmonary oedema. By contrast, the cardiac parameters SVI, GEF and GEDVI were comparable in both cohorts. High EVLWI values were associated with viral persistence, prolonged intensive care treatment and in-hospital mortality (23.2 ± 6.7% vs. 30.3 ± 6.0%, p = 0.025). Also, EVLWI showed a significant between-subjects (r = − 0.60; p = 0.001) and within-subjects correlation (r = − 0.27; p = 0.028) to Horowitz index. Compared to non COVID-19 ARDS, COVID-19 results in markedly elevated EVLWI-values in patients with ARDS. High EVLWI reflects a non-cardiogenic pulmonary oedema in COVID-19 ARDS and could serve as parameter to monitor ARDS progression on ICU.
BAFF, APRIL and BAFFR on the pathogenesis of Immunoglobulin-A vasculitis
Authors: Prieto-Peña Diana , Genre Fernanda , Remuzgo-Martínez Sara , Pulito-Cueto Verónica , Atienza-Mateo Belén , Llorca Javier , Sevilla-Pérez Belén , Ortego-Centeno Norberto , Lera-Gómez Leticia , Leonardo María Teresa , Peñalba Ana , Narváez Javier , Martín-Penagos Luis , Rodrigo Emilio , Miranda-Filloy José A. , Caminal-Montero Luis , Collado Paz , Pérez Javier Sánchez , de Argila Diego , Rubio Esteban , Luque Manuel León , Blanco-Madrigal Juan María , Galíndez-Agirregoikoa Eva , Gualillo Oreste , Martín Javier , Castañeda Santos , Blanco Ricardo , González-Gay Miguel A. , López-Mejías Raquel 
Published: June 2021

AbstractBAFF, APRIL and BAFF-R are key proteins involved in the development of B-lymphocytes and autoimmunity. Additionally, BAFF, APRIL and BAFFR polymorphisms were associated with immune-mediated conditions, being BAFF GCTGT>A a shared insertion-deletion genetic variant for several autoimmune diseases. Accordingly, we assessed whether BAFF, APRIL and BAFFR represent novel genetic risk factors for Immunoglobulin-A vasculitis (IgAV), a predominantly B-lymphocyte inflammatory condition. BAFF rs374039502, which colocalizes with BAFF GCTGT>A, and two tag variants within APRIL (rs11552708 and rs6608) and BAFFR (rs7290134 and rs77874543) were genotyped in 386 Caucasian IgAV patients and 806 matched healthy controls. No genotypes or alleles differences were observed between IgAV patients and controls when BAFF, APRIL and BAFFR variants were analysed independently. Likewise, no statistically significant differences were found in the genotype and allele frequencies of BAFF, APRIL or BAFFR when IgAV patients were stratified according to the age at disease onset or to the presence/absence of gastrointestinal (GI) or renal manifestations. Similar results were disclosed when APRIL and BAFFR haplotypes were compared between IgAV patients and controls and between IgAV patients stratified according to the clinical characteristics mentioned above. Our results suggest that BAFF, APRIL and BAFFR do not contribute to the genetic network underlying IgAV.
Surgical outcomes of re-excimer laser phototherapeutic keratectomy (re-PTK)
Authors: Hieda Osamu , Sotozono Chie , Nakamura Yo , Wakimasu Koichi , Kinoshita Shigeru 
Published: June 2021

AbstractTo assess the surgical outcomes of re-excimer laser phototherapeutic keratectomy (re-PTK) for recurrent disease after initial PTK. Retrospective cohort study with historical comparison group. This study involved 56 patients who underwent re-PTK (mean follow-up period: 47.9 ± 36.2 months) at the Baptist Eye Institute, Kyoto, Japan. In all subjects, corrected-distance visual acuity (CDVA) before and after re-PTK was compared. Postoperative recurrence of corneal lesion with a decrease of CDVA of two lines or more was compared with postoperative best CDVA deemed as a significant relapse. The Kaplan–Meier method was used to compare the recurrence rate post-re-PTK with that after the initial PTK. The disease classification in the 78 eyes was heterozygous-type GCD (hetero-GCD, n = 47 eyes), homozygous-type GCD (homo-GCD, n = 13 eyes), band keratopathy (n = 7 eyes), lattice corneal dystrophy (n = 6 eyes), and other (n = 5 eyes). After re-PTK, homo-GCD recurred statistically significantly earlier than hetero-GCD (P = 0.0042). No significant difference was found in the recurrence rate for all diseases between post-re-PTK and post initial PTK (P > 0.05). Surgical outcomes after re-PTK were nearly equal to those after initial-PTK.
Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
Authors: Park Ra-Mi , Nguyen Ngoc-Han Thi , Lee Su-Min , Kim Yang-Hoon , Min Jiho 
Published: June 2021

AbstractThe objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides. Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes. The dissolution test confirmed the availability of the oral dosage form. When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced. However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained. At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome. When the pH was increased, alginate oligosaccharides were dissolved and the lysosome was released. SDS–polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h. The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation
Authors: Jana Barbara , Całka Jarosław 
Published: June 2021

AbstractUterine inflammation is a very common and serious condition in domestic animals. To development and progression of this pathology often lead disturbances in myometrial contractility. Participation of β1-, β2- and β3-adrenergic receptors (ARs) in noradrenaline (NA)-influenced contractility of the pig inflamed uterus was studied. The gilts of SAL- and E.coli-treated groups were administered saline or E.coli suspension into the uterine horns, respectively. Laparotomy was only done in the CON group. Compared to the period before NA administration, this neurotransmitter reduced the tension, amplitude and frequency in uterine strips of the CON and SAL groups. In the E.coli group, NA decreased the amplitude and frequency, and these parameters were lower than in other groups. In the CON, SAL and E.coli groups, β1- and β3-ARs antagonists in more cases did not significantly change and partly eliminated NA inhibitory effect on amplitude and frequency, as compared to NA action alone. In turn, β2-ARs antagonist completely abolished NA relaxatory effect on these parameters in three groups. Summarizing, NA decreases the contractile amplitude and frequency of pig inflamed uterus via all β-ARs subtypes, however, β2-ARs have the greatest importance. Given this, pharmacological modulation of particular β-ARs subtypes can be used to increase inflamed uterus contractility.
Texting Behaviors of Individuals With Chronic Aphasia: A Descriptive Study
Authors: Kinsey Laura E. , Lee Jaime B. , Larkin Elissa M. , Cherney Leora R. 
Published: June 2021

Purpose:
In today's digital world, text messaging is one of the most widely used ways that people stay connected. Although it is reported that people with aphasia experience difficulties with texting, little information is available about how they actually do text. This study reports texting behaviors, such as the number and type of messages sent and contacts individuals with aphasia have. The relationships between texting behaviors and aphasia severity, including writing impairments, and social connectedness are explored.

Method:
Twenty participants were sampled from an ongoing randomized clinical trial investigating an electronic writing treatment for aphasia (Clinical Trials Identifier: NCT03773419). Participants provided consent for researchers to view and analyze texts sent and received over a 7-day period immediately prior to the assessment. Participants' text messages were recorded, transcribed verbatim, and coded.

Results:

Over the 7-day period, the number of contacts with whom participants texted ranged from one to 18. The mean number of text messages exchanged was 40.3 (
SD
= 48.24), with participants sending an average of 15.4 (
SD
= 23.45) texts and receiving an average of 24.9 (
SD
= 29.44) texts. Participants varied in the types of texts sent; some had a larger proportion of initiated texts, while others drafted more responses, either simple or elaborative in nature. There was no correlation between the total number of texting exchanges and the Western Aphasia Battery–Revised Aphasia Quotient (

r
s

= .13,
p
= . 29) or the Western Aphasia Battery–Revised Writing subtest (

r
s

= .05,
p
= .42). There was also no correlation between the total number of texting exchanges and scores on measures of social connectedness.

Conclusions:
Texting behaviors of individuals with aphasia are widely variable. Demographics, severity of aphasia and writing, and social connectedness may not predict texting behaviors. Therefore, it is clinically important to explore the unique texting abilities and preferences of each individual to meet their communication and social participation goals.

Supplemental Material

https://doi.org/10.23641/asha.14669664