bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the leaf metabolome in Arabidopsis thaliana mutation accumulation lines reveals association of pleiotropy and fitness consequences
Authors: Kreutzmann Sydney , Pompa Elizabeth , Ngyuen Nhan , Tilahun Liya , Rutter Matthew , Weng Mao-Lun , Fenster Charles , Olson-Manning Carrie F 
Published: December 2021

Abstract
Understanding the mechanisms by which mutations affect fitness and the distribution of mutational effects are central goals in evolutionary biology. Mutation accumulation (MA) lines have long been an important tool for understanding the effect of new mutations on fitness, phenotypic variation, and mutational parameters. However, there is a clear gap in predicting the effect of specific new mutations to their effects on fitness. Here, we complete gene ontology analysis and metabolomics experiments on Arabidopsis thaliana MA lines to determine how spontaneous mutations directly affect global metabolic output in lines that have measured fitness consequences. For these analyses, we compared three lines with relative fitness consistently higher than the unmutated progenitor and three lines with lower relative fitness as measured in four different field trials. In a gene ontology analysis, we find that the high fitness lines were significantly enriched in mutations in or near genes with transcription regulator activity. We also find that although they do not have an average difference in the number of mutations, low fitness lines have significantly more metabolic subpathways disrupted than high fitness lines. Taken together, these results suggest that the effect of a new mutation on fitness depends less on the specific metabolic pathways disrupted and more on the pleiotropic effects of those mutations, and that organisms can explore a considerable amount of physiological space with only a few mutations.
A biosurfactant-producing yeast Rhodotorula sp.CC01 utilizing landfill leachate as nitrogen source and its broad degradation spectra of petroleum hydrocarbons
Authors: Lin Xiaoyun , Zhou Hanghai , Zeng Feng , Jiang Lijia , Atakpa Edidiong Okokon , Zhang Chunfang , Xie Qinglin 
Published: December 2021

Abstract
In this study, a biosurfactant producing strain, Rhodotorula sp. CC01 was isolated using landfill leachate as nitrogen source, while olive oil was determined as the best sole carbon source for producing biosurfactants. The biosurfactant produced by Rhodotorula sp. CC01 was characterized as glycolipids with a critical micelle concentration of 70 mg/L, which showed stability over a wide range of pH (2–12), salinity (0–100%), and temperature (20–100°C). During the cultivation process, the surface tension decreased from 51.87 to 28.20 mN/m in 15 h, and the removal efficiency of NH4+-N reached 84.2% after 75 h cultivation with a maximum NH4+-N removal rate of 3.92 mg·L-1·h−1. In addition, Rhodotorula sp. CC01 has proven to be of great potential in remediating petroleum hydrocarbons, as revealed by chromogenic assays. The findings of this study prove a cost-effective strategy for the production of BS by yeast through the utilization of landfill leachate.
Analysis of The Global Posture of College Students With Chronic Neck Pain
Authors: Zhong Jiugen , Wang Wenhao , Yu Ligen , Hou Xiaohui 
Published: December 2021

Abstract
Background: Chronic neck pain (CNP) is common, but methods that focus on the cervical spine have not met the patients' medical expectations.Objective: To investigate the global postural difference between students with CNP and healthy people.Design: Cross-sectional study.Methods: Twenty-seven healthy college students without neck pain and 31 students with CNP were recruited and allocated into a control group and a CNP group. Differences in standing postural indicators between the two groups were compared.Results: Compared to the control group, the leg length discrepancy and the right rearfoot angle were larger and the anterior lower limb alignment angle was smaller. In the sagittal plane, the left sagittal lower limb alignment and right cervical alignment angles were larger, while the left and right sagittal body alignment angles in the CNP group were smaller. The odds ratio calculation for the trunk forward lean, right foot valgus, and knee flexion on both sides indicated that these are risk factors for CNP, while knee varus is not a risk factor for CNP. The remaining abnormal postures were shown not to be associated with CNP.Limitation: This study did not conduct in-depth research on the physiological state of the muscles, joints, and other structures, and we did not apply these theories to practice.Conclusions: Abnormal posture in students with CNP is mainly concentrated in the sagittal plane. Trunk forward lean, foot valgus, and knee flexion on both sides are risk factors for CNP.
Maintenance Status and Availability of Public Automated External Defibrillators: Results of On-Site Inspection
Authors: Kim Tae Youn , Jung Yun-kyoung , Yoon Sun Hwa , Kim Sun Ju , Cha Kyoung-chul , Jung Woo Jin , Roh Young Il , Kim Soyeong , Kim Sung Hwa , Kang Dae Ryong , Hwang Sung Oh 
Published: December 2021

Abstract
Objective

This study aimed to assess the maintenance status and availability of publicly installed automated external defibrillators (AEDs).
Methods

Public AEDs installed in Seoul 2013 to 2017 were included. An inspector checked the maintenance status and availability of AEDs annually using a checklist.
Results

A total of 23,619 AEDs were inspected for 5 years. Access to AEDs was improved, including reduced obstacles around AEDs (from 9.3% in 2013 to 0.8% in 2017) and increased AED signs (from 34.3% in 2013 to 91.2% in 2017). The number of AEDs in normal operation (from 94.0% in 2013 to 97.5% in 2017), with normal battery charge (from 95.6% in 2013 to 96.8% in 2018), and electrode availability increased (from 97.1% in 2013 to 99.0% in 2017). However, the rate of electrode validity decreased (from 90.0% in 2013 to 87.2% in 2017). Non-ready-to-use AEDs and AEDs with limited 24-h availability accounted for 15.4% and 44.1% of the total number of AEDs, respectively.
Conclusions

Although most AEDs had a relatively good maintenance status, a significant proportion of public AEDs was not available for 24-h use. Invalid electrodes and limited 24-h accessibility were the main reasons that limited the 24-h availability of public AEDs.
Climate Change Increased The Compound Extreme Precipitation-Flood Events In A Representative Watershed of The Yangtze River Delta, China
Authors: He Yuxiu , Wang Qiang , Xu Youpeng , Li Ziyi , Yuan Jia , Lu Miao , Lin Zhixin 
Published: December 2021

Abstract
A compound perspective on hydrological extreme events is of paramount significance as it may lead to damages with larger losses. In this study, an integrated framework, based on downscaled climate variables and hydrological model, i.e. the Soil and Water Assessment Tool, was applied to generate extreme precipitation (Rx1day) and extreme streamflow (Sx1day) series under historical and future climate conditions. Then the potential impacts of climate change for univariate and bivariate joint frequency of extreme precipitation and flood in Xitiaoxi River Basin (XRB), a representative watershed of the Yangtze River Delta, is detected. The compound risk of extreme precipitation and flood under different levels of joint return period for historical and projected periods are estimated by copula‐based two-dimensional approaches. Major findings can be summarized: (1) The Rx1day and Sx1day under future scenarios increased by -0.4 ~ 11.7% and 0.7 ~ 20.4%, respectively, compared to historical period based on univariate frequency analysis, indicating the increasing magnitude of the flood in the future. (2) Climate change with different emission scenarios all have a driving effect on the rising coactivity of extreme precipitation and flood under compound flooding frequency analysis. In addition, the enhancement of climate change to extreme events is more apparent for extremes with higher return period and under the periods of 2080s. (3) Moreover, the flood frequency designs are deduced by bivariate joint distribution are safer than that by univariate distribution. This study may provide actionable insights to formulate the planning scheme of flood control and disaster reduction under the changing environment.
ABCC10-Mediated Oxaliplatin Resistance in Colorectal Cancer Was Alleviated by Intense Endoplasmic Reticulum Stress (ERS) / IRE1α
Authors: Liu Xiaohui , Chen Hong , Sun Haimei , Guo Xiaoxia , Wu Bo , Sun Tingyi , Ji Fengqing , Yang Shu , Zhou Deshan 
Published: December 2021

Abstract
Background: Oxaliplatin resistance is a challenge in treating colorectal cancer (CRC) patients, contributory to the failure in chemotherapy. However, the mechanism of Oxaliplatin resistance has not been completely elucidated. In this study, we explored the key molecule involved in the Oxaliplatin resistance, which could be a candidate therapeutic target to attenuate chemo-resistance in CRC cells.Methods: Microarray screening, western blot and qPCR on clinic CRC samples were conducted to select the target gene ABCC10. The Oncomine Oncology Database and the Cancer Genome Atlas (TCGA) data were analyzed to figure out the correlation between the clinical manifestation and ABCC10. ABCC10 knock-down in CRC cells was conducted to identify its role in the Oxaliplatin resistance. Cell counting kit-8 (CCK-8) assay was conducted to identify the CRC cell viability and Oxaliplatin IC50. Flow cytometry was conducted to detect the cell apoptosis exposed to Oxaliplatin. The intracellular Oxaliplatin accumulation was measured by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS).Results: ABCC10 transporter was correlated with CRC relapse and metastasis, indicating a CRC-promoting effect of ABCC10. In ABCC10 knock-down CRC cells the Oxaliplatin sensitivity was significantly elevated due to an increase of intracellular Oxaliplatin accumulation resulted from the diminished drug efflux. We next explored a strategy to inhibit ABCC10 in CRC cells, paying a special interest in the endoplasmic reticulum stress (ERS) / unfolded protein response (UPR) that plays a dual role in tumor development. We found that the CRC cell viability was profoundly decreased and the pro-apoptotic factor CHOP and apoptosis were increased by the intense ERS/UPR instead of the inhibited and mild ERS/UPR. Significantly, the Oxaliplatin sensitivity of CRC cells was enhanced in response to the intense ERS, which was blocked by inhibiting IRE1α branch of UPR. Finally, we figured out that the intense ERS/UPR down-regulated ABCC10 via regulated IRE1-dependent decay (RIDD) activity. Conclusion: Oxaliplatin was a substrate of ABCC10. The intense ERS/IRE1α elicited anti-CRC effects through down-regulating ABCC10 so as to increase Oxaliplatin sensitivity, in addition to inducing CHOP. We suggested that introduction of intense ERS/UPR could be a promising strategy to restore chemo-sensitivity when used in combination with Oxaliplatin or other chemotherapeutic drugs pumped out by ABCC10.
Integrated Analysis of lncRNA-miRNA-mRNA ceRNA Network in Human Diarrhea Irritable Bowel Syndrome
Authors: LIANG Jun-wei , BAI Wen-jun , WANG Xiao-yan , CHI Li-li 
Published: December 2021

Abstract
Background:Many studies on long chain non-coding RNAs (lncRNAs) are published in recent years. But the roles of lncRNAs in diarrhea irritable bowel syndrome (IBS-D) are still unclear and should be further examined. The present work focused on determining the molecular mechanisms underlying lncRNAs regulation in IBS-D on the basis of the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network.Methods:This study collected the mRNAs (GSE36701) expression data within human tissue samples with IBS-D group and normal group based on Gene Expression Omnibus (GEO) database and collected the differentially expressed lncRNAs (DELs) and differentially expressed miRNAs (DEmiRs) based on PubMed.Functional enrichment analysis of DEGs was performed on the DAVID database. Then the interaction network was constructed and visualized using STRING database and Cytoscape.Results: This study identified 3192 DEmRNAs (1437 with up-regulation and 1755 with down-regulation),29 DEmiRs (18 upregulated and 11 downregulated)and 2 DELs(one upregulated and one downregulated) between IBS-D and control samples.Furthermore,we constructed a lncRNA-miRNA-mRNA network through two DELs (lncRNA TUG1 with up-regulation and lncRNA H19 with down-regulation), four DemiRs (hsa-miR-148a-3p,hsa-miR-342-3p,hsa-miR-149-5p with up-regulation and hsa-miR-219a-5p with down-regulation)and 24 DEGs (4 with up-regulation and 20 with down-regulation) with 42 axes. Simultaneously, we conducted functional enrichment and pathway analyses on genes within the as-constructed ceRNA network. According to our PPI/ceRNA network and functional enrichment analysis results, two critical genes were found (BCL2L11 and QKI). Conclusion:In conclusion, the ceRNA interaction axis we identified is a potentially critical target for treating IBS-D.BCL2L11 axis(LncH19-hsa-miR-148a-3p-BCL2L11) may via interaction with PI3K/AKT pathways in IBS-D.Our results shed more lights on the possible pathogenic mechanism in IBS-D using a lncRNA-associated ceRNA network.
Does Predicted Age At Peak Height Velocity Explain Physical Performance In U13-15 Basketball Female Players?
Authors: Gryko Karol , Adamczyk Jakub Grzegorz , Kopiczko Anna , Calvo Jorge Lorenzo , Calvo Alberto Lorenzo , Mikołajec Kazimierz 
Published: December 2021

Abstract
Background: The aims of the study were (i) to identify the physical fitness and basic anthropometric characteristics of Polish female basketball players aged 13 to 15 years, (ii) to show the effect of maturity timing on the performance in motor tests and basic body composition parameters, (iii) to identify the index that contributes most to the prediction of performance in the tests of speed, jumping ability, agility, and endurance. Methods: The sample included 925 female Polish players (U13-15). In part 1, maturity timing category distribution were examined within across age-groups. In part 2, the relationship between the anthropometric variables, physical fitness performance was assessed based on maturity timing categories (ANCOVA analysis). In part 3, backward stepwise multiple regression analyse quantified the relationship between maturity timing (group of PHV) and physical performance.Results: ANCOVA results (age, body height, and body mass as covariates) showed in the U13 female basketball players significantly higher sprinting (20m), jumping ability and endurance tests results of the PHV1 group.Better results was observed in U14 female players in PHV1 compared to PHV2 and PHV3 in 20m and jumping tests but opposite trend was observed for 5m sprint and endurance test (distance covered and VO2max). U15 basketball players from the PHV3 group were characterized by better results of jumping abilities, endurance, 10m and 20m sprint and agility (total, S4) tests. Maturity timing (10m), chronological age (5 m, 20 m, agility, SVJ, VJ, and VO2max tests), body height (10m), body mass (10m, 20m, VJ, VO2max), and the interaction between body mass and height (SVJ) were significant (adjusted R2 = 0.02-0.10; p < 0.001) predictors of motor skills. Conclusion: The results can help the coaches to personalize training programs and to adapt the training content to the biological age of the players.
High Expression of SRD5A3 in Human Hepatocellular Carcinoma (HCC) Predicts Poor Prognosis: A Study Based on TCGA Data
Authors: Xue Jing , Yang Xianzhao , Jiang Feng 
Published: December 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death worldwide. Steroid 5 alpha-reductase 3 (SRD5A3) was reported to be up-regulated in many types of cancer. However, its expression and role in HCC remains to be elucidated. We aim to evaluate the significance of SRD5A3 expression in HCC by using analysis of a public dataset from The Cancer Genome Atlas (TCGA).Methods: The relationship between clinical pathologic features and SRD5A3 were analyzed with the Kolmogorov‐Smirnov test and the logistic regression. Cox regression and the Kaplan-Meier method were used to assess the clinicopathologic characteristics associated with overall survival (OS) in TCGA patients. In addition, GSEA was used to predict potential hallmarks associated with different expression of SRD5A3 on transcriptional sequences from TCGA database.Results: SRD5A3 was highly expressed in HCC tumor tissue compared to normal tissue. A total of 184 upregulated DEGs (differentially expressed genes) and 58 downregulated DEGs were identified between high expression and low expression of SRD5A3. Among them, 22 hub genes mainly belonging to the keratin and MUC family demonstrated by connectivity degree in the PPI network were screened out. Kaplan-Meier method showed that HCC patients in the high SRD5A3 expression group had poorer overall survival (OS, HR=2.26(1.58-3.24), p<0.001). In addition, cell cycle mitotic, cell cycle checkpoints, mitotic nuclear division, Q-glycan processing, protein O-linked glycosylation were differentially enriched in the high SRD5A3 expression phenotype pathway. In addition, SRD5A3 expression level has significant correlations with infiltrating levels of Th17 (R = -0.238, p < 0.001), Cytotoxic cells (R = -0.234, p < 0.001) and Th2 cells (R = 0.258, p < 0.001) in HCC.Conclusions: High expression of SRD5A3 was significantly correlated with poor prognosis in HCC patients. It may be a potential biomarker in HCC.
Silencing p62 reduces ox-LDL-Induced M1 Polarization and Inflammation in Macrophages by Inhibiting mTOR/NF-κB Signaling Pathways
Authors: Yang Wei , Su Guangming , Liu Yanhong 
Published: December 2021

Abstract
Background: Macrophages can change their phenotypes according to the changes in the microenvironment, and thus have various functions, that is, macrophages polarization. Macrophage phenotype is associated with the progression of atherosclerotic plaques. Studies have shown a large accumulation of p62 protein in atherosclerotic plaques. Whether the accumulation of p62 protein affects the level of macrophage polarization and inflammation and its mechanism is not clear.Methods: The p62 levels of macrophages treated with ox-LDL were detected by western blotting and qRT-PCR. Several polarizing markers and cytokines associated with atherosclerosis were detected by western blotting, ELISA, qRT-PCR, and flow cytometry to assess macrophage phenotype. The effect of p62 on the treatment of macrophage polarization by ox-LDL was studied by silencing p62 by gene silencing technique. The activity of mTOR and NF-κB signaling pathways was evaluated by detecting p-mTOR and intranuclear p65 levels in western blotting to explore the mechanism of p62. Rapamycin inhibited mTOR to demonstrate its role in activating the NF-κB signaling pathway and in ox-LDL therapy of p62 induced M1 polarization in macrophages.Results: ox-LDL induced a significant increase in p62 and an increase in M1 markers and inflammatory cytokines. After p62 silencing, M1 markers and inflammatory cytokines decreased significantly, while M2 markers and anti-inflammatory cytokines increased significantly. Silencing p62 inhibited p-mTOR and p65 nuclear translocation. Rapamycin inhibited ox-LDL-induced p65 nuclear translocation and M1 markers, and increased M2 markers.Conclusion: p62 protein accumulation in ox-LDL treatment macrophages induces M1 polarization and inflammatory cytokines through the mTOR/NF-κB pathway.
Responses of CO2 Emissions and Soil Microbial Community Structures to Organic Amendment in Two Contrasting Soils in Zambia
Authors: Hamamoto Toru , Nhamo Nhamo , Chikoye David , Mukumbuta Ikabongo , Uchida Yoshitaka 
Published: December 2021

Abstract
In sub-Saharan Africa, efforts have been made to increase soil carbon (C) content in agricultural ecosystems, due to severe soil degradation. The use of organic materials is one of the realistic methods to recover soil C. However, the impacts of organic amendments on soil microbial community and C cycles under limited soil C conditions are still unknown. We conducted field experiments using organic amendments in two sites with contrasting C content in Zambia. At both sites, temporal changes of soil carbon dioxide (CO2) emissions, bacterial and archaeal community structures were monitored during crop growing season (126 days). The organic amendments increased CO2 emissions with increased bacterial and archaeal abundance in the Kabwe site, while no impacts were shown in the Lusaka site. We also observed larger temporal variability in soil microbial community structure in Kabwe than in Lusaka. These contrasting results between the two soils might be due to the gap in microbial community stability. However, organic amendments have a significant potential to enhance microbial abundance and consequently sequester soil C in the Kabwe site. Site-specific strategies are needed to deal with the issues of soil C depletion in drylands.
TP73-AS1 as a Predictor of Clinicopathological Parameters and Prognosis in Human Malignancies: A Meta and Bioinformatics Analysis
Authors: Chen Caizhi , Wang Jingjing , Feng Yeqian , Liang Ye , Huang Yan , Zou Wen 
Published: December 2021

Abstract
Background: LncRNA TP73-AS1 is dysregulated in various tumors but the correlation between its expression and clinicopathological parameters and/or prognoses in cancer patients is inconclusive. Here, we performed a meta-analysis to evaluate the prognostic value of lncRNA TP73-AS1 for malignancies.Methods: We systematically searched four online databases including PubMed, the Web of Science, Embase, and the Cochrane Library for eligible articles published up to June 29/2020. Odds ratios (ORs) and Pooled hazard ratios (HRs) with 95% confidence intervals (95% CIs) were used to assess the association of TP73-AS1 expression with prognostic and clinicopathological parameters. We further validated TP73-AS1 expression in various malignancies and its potential prognostic value using the GEPIA online database. We predicted potential biological processes and relevant signal mechanisms through the public databases.Results: A total of 26 studies including 1770 patients were analyzed to evaluate the relationship between TP73-AS1 expression, clinicopathological features and prognostic indicators. The results indicated that TP73-AS1 expression markedly correlates with TNM stage, tumor size, lymph node metastasis and distant metastasis. No correlation with age, gender or differentiation was observed. TP73-AS1 overexpression was a biomarker of poor Overall survival (OS) and Disease-Free-Survival (DFS). Dysregulated TP73-AS1 expression and its prognostic value in various cancers was validated based on The Cancer Genome Atlas (TCGA). Further biological function predictions indicated that TP73-AS1 was involved in pro-oncogenic signaling.Conclusions: The upregulation of LncRNA TP73-AS1 was related to detrimental clinicopathological parameters and can be considered an indicator of poor prognosis for cancer malignancies.