bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of training on forced submersion responses in harbor seals
Authors: Jobsis P. D. , Ponganis P. J. , Kooyman G. L. 
Published: April 2021

SUMMARY
In several pinniped species, the heart rates observed during unrestrained dives are frequently higher than the severe bradycardias recorded during forced submersions. To examine other physiological components of the classic ‘dive response’ during such moderate bradycardias, a training protocol was developed to habituate harbor seals (Phoca vitulina) to short forced submersions. Significant changes were observed between physiological measurements made during naive and trained submersions (3–3.5 min). Differences were found in measurements of heart rate during submersion (naive 18±4.3 beats min–1versus trained 35±3.4 beats min–1), muscle blood flow measured using laser-Doppler flowmetry (naive 1.8±0.8 ml min–1 100 g–1versus trained 5.8±3.9 ml min–1 100 g–1), change in venous PO2 (naive –0.44±1.25 kPa versus trained –1.48±0.76 kPa) and muscle deoxygenation rate (naive –0.67±0.27 mvd s–1versus trained –0.51±0.18 mvd s–1, a relative measure of muscle oxygenation provided by the Vander Niroscope, where mvd are milli-vander units). In contrast to the naive situation, the post-submersion increase in plasma lactate levels was only rarely significant in trained seals. Resting eupneic (while breathing) heart rate and total oxygen consumption rates (measured in two seals) were not significantly different between the naive and trained states. This training protocol revealed that the higher heart rate and greater muscle blood flow in the trained seals were associated with a lower muscle deoxygenation rate, presumably secondary to greater extraction of blood O2 during trained submersions. Supplementation of muscle oxygenation by blood O2 delivery during diving would increase the rate of blood O2 depletion but could prolong the duration of aerobic muscle metabolism during diving. This alteration of the dive response may increase the metabolic efficiency of diving.
Regulation of intracellular pH in anoxia-tolerant and anoxia-intolerant teleost hepatocytes
Authors: Krumschnabel Gerhard , Manzl Claudia , Schwarzbaum Pablo J. 
Published: April 2021

SUMMARY
Mechanisms of intracellular pH (pHi) regulation were investigated in anoxia-tolerant hepatocytes from goldfish Carassius auratus, and compared to the situation in the anoxia-intolerant hepatocytes from trout Oncorhynchus mykiss. Under normoxic conditions, the pHi of goldfish hepatocytes was regulated by a Na+/H+ exchanger and a Na+-independent Cl–/HCO3– exchanger, the latter being activated only after acidification of the cells. Mechanisms of acid secretion appear to be fuelled, at least in part, by lactate formation under fully aerobic conditions, as inhibition of glycolysis caused a drastic reduction of steady state proton release. In trout hepatocytes both a Na+/H+ exchanger and a Cl–/HCO3– exchanger were found to be tonically active, as described previously. During chemical anoxia a constant pHi was maintained in goldfish hepatocytes, whereas it was reversibly reduced by 0.3 units in the trout cells. Under these conditions a reversible increase in the rate of acid secretion was induced in the cells from both species. In the goldfish cells this was based on a SITS-sensitive transporter, possibly involving export of lactate, with no contribution from Na+/H+ exchange. By contrast, in hepatocytes from trout, CN-induced acid secretion was dominated by the activity of the Na+/H+ exchanger. Brief exposure to extracellular acidosis had no dramatic effects on the energetics of hepatocytes from either species.
Evidence for a mineralocorticoid-like receptor linked to branchial chloride cell proliferation in freshwater rainbow trout
Authors: Sloman Katherine A. , Desforges Patrick R. , Gilmour Kathleen M. 
Published: April 2021

SUMMARY
Fish acclimated to ion-deficient water exhibit proliferation of branchial chloride cells. The objective of the present study was to investigate the role of cortisol in this response using the corticosteroid receptor antagonists RU486 and spironolactone. RU486 is a potent antagonist of the glucocorticoid actions of cortisol, whereas spironolactone exhibits high-affinity binding to mineralocorticoid receptors, with a resulting blockade of mineralocorticoid properties in mammals. Untreated rainbow trout, as well as rainbow trout given a single intraperitoneal implant of coconut oil alone, coconut oil containing RU486 (0.5 mg g–1) or coconut oil containing spironolactone (0.1 mg g–1), were exposed to either dechlorinated city-of-Ottawa tapwater or artificial softwater for 7 days. Neither corticosteroid antagonist nor acclimation condition affected circulating plasma cortisol levels, plasma ion concentrations or gill Na+-K+-ATPase activity. Kidney Na+-K+-ATPase activity was significantly higher in softwater-acclimated fish than in fish held in dechlorinated tapwater. In addition, whereas RU486 treatment was found to be without effect on gill morphometrics, treatment with spironolactone inhibited the proliferation of chloride cells normally associated with acclimation to ion-deficient water. The results of the present study provide further evidence for the mineralocorticoid actions of cortisol in freshwater fish, specifically in eliciting chloride cell proliferation. Furthermore, these results support the hypothesis that distinct glucocorticoid and mineralocorticoid receptor populations are present in teleost fish, despite the apparent absence of the classic mineralocorticoid hormone, aldosterone.
Na++K+-ATPase in gills of the blue crabCallinectes sapidus: cDNA sequencing and salinity-related expression of α-subunit mRNA and protein
Authors: Towle David W. , Paulsen Ryan S. , Weihrauch Dirk , Kordylewski Marek , Salvador Cristina , Lignot Jean-Hervé , Spanings-Pierrot Céline 
Published: April 2021

SUMMARYMany studies have shown that hyperosmoregulation in euryhaline crabs is accompanied by enhanced Na++K+-ATPase activity in the posterior gills, but it remains unclear whether the response is due to regulation of pre-existing enzyme or to increased gene transcription and mRNA translation. To address this question, the complete open reading frame and 3′ and 5′ untranslated regions of the mRNA coding for the α-subunit of Na++K+-ATPase from the blue crab Callinectes sapidus were amplified by reverse transcriptase/polymerase chain reaction (RT-PCR) and sequenced. The resulting 3828-nucleotide cDNA encodes a putative 1039-amino-acid protein with a predicted molecular mass of 115.6 kDa. Hydrophobicity analysis of the amino acid sequence indicated eight membrane-spanning regions, in agreement with previously suggested topologies. The α-subunit amino acid sequence is highly conserved among species, with the blue crab sequence showing 81–83 % identity to those of other arthropods and 74–77 % identity to those of vertebrate species. Quantitative RT-PCR analysis showed high levels of α-subunit mRNA in posterior gills 6–8 compared with anterior gills 3–5. Western blots of gill plasma membranes revealed a single Na++K+-ATPase α-subunit protein band of the expected size. The posterior gills contained a much higher level of α-subunit protein than the anterior gills, in agreement with previous measurements of enzyme activity. Immunocytochemical analysis showed that the Na++K+-ATPase α-subunit protein detected by α5 antibody is localized to the basolateral membrane region of gill epithelial cells. Transfer of blue crabs from 35 to 5 ‰ salinity was not accompanied by notable differences in the relative proportions of α-subunit mRNA and protein in the posterior gills, suggesting that the enhanced Na++K+-ATPase enzyme activity that accompanies the hyperosmoregulatory response may result from post-translational regulatory processes.
The effects of depth on the cardiac and behavioural responses of double-crested cormorants (Phalacrocorax auritus) during voluntary diving
Authors: Enstipp Manfred R. , Andrews Russel D. , Jones David R. 
Published: April 2021

SUMMARYHeart rate and dive behaviour were monitored in double-crested cormorants (Phalacrocorax auritus) during shallow (1 m) and deep diving (12 m), after breathing different gas mixtures, to investigate the role of depth and the accompanying changes in blood gas levels in cardiac and behavioural control during voluntary diving. Pre-dive heart rate in both shallow- and deep-diving birds was approximately three times the resting heart rate (137.9±17.5 beats min–1; mean ± s.d., N=5), falling abruptly upon submersion to around 200–250 beats min–1. During shallow diving, the initial reduction in heart rate was followed by a secondary, more gradual decline, to around the resting level. In contrast, during deep diving, heart rate stabilised at 200–250 beats min–1. In dives of similar duration, mean dive heart rate was significantly lower during shallow diving (163.2±14.0 beats min–1) than during deep diving (216.4±7.7 beats min–1), but in both cases was significantly above the resting value. The difference in cardiac response is probably due to an increase in arterial oxygen tension (PaO2) during the descent phase of deep dives (compression hyperoxia). Exposure to a hyperoxic gas mixture before shallow diving significantly increased mean dive heart rate, while exposure to a hypoxic gas mixture in both the shallow and deep dive tanks significantly reduced mean dive heart rate. In contrast, breathing hypercapnic gas before diving had no significant effect on dive heart rate. We suggest that the cardiac response to voluntary diving in double-crested cormorants is strongly influenced by changes in blood oxygen levels throughout the dive. Dive duration was unaffected by alterations in inspired gas composition, but surface interval duration decreased during hyperoxic gas exposure and increased during hypoxic gas exposure. The most efficient dive pattern (highest dive/pause ratio) was observed after hyperoxic exposure. Our study suggests that blood oxygen level is a powerful stimulus that facilitates the cardiac and behavioural adjustments during foraging that are important components of a strategy allowing double-crested cormorants to maximise the time spent under water and, hence, potential foraging time.
The role of angiotensin II in regulating catecholamine secretion during hypoxia in rainbow troutOncorhynchus mykiss
Authors: Lapner Katherine N. , Perry Steve F. 
Published: April 2021

SUMMARYExperiments were performed in vivo on chronically cannulated adult rainbow trout (Oncorhynchus mykiss) to assess the involvement of serotonergic or muscarinic receptor stimulation or activation of the renin–angiotensin system in eliciting catecholamine release during acute hypoxia during periods of nicotinic receptor desensitisation.Despite nicotinic receptor desensitisation induced by intravenous infusion of nicotine (1.3×10–5 mol kg–1 h–1), plasma catecholamine levels were increased to levels (adrenaline plus noradrenaline 125–200 nmol l–1) similar to those in control fish during severe hypoxia (40–45 mmHg; 5.3–6.0 kPa). Blockade of serotonergic receptors using methysergide or of muscarinic receptors using atropine did not affect the ability of fish to elevate circulating catecholamine levels during hypoxia. However, selective blockade of the renin–angiotensin system, using lisinopril to inhibit angiotensin-converting enzyme, prevented the elevation of both angiotensin II and circulating catecholamine levels in acutely hypoxic fish experiencing nicotinic receptor desensitisation. In fish possessing functional nicotinic receptors, angiotensin-converting enzyme blockade attenuated but did not prevent the elevation of plasma catecholamine levels during hypoxia. The results of this study indicate that the renin–angiotensin system is activated during hypoxia and plays a role in eliciting catecholamine release that is secondary to activation of nicotinic receptors. However, under conditions of nicotinic receptor desensitisation, activation of the renin–angiotensin system during hypoxia is a prerequisite for catecholamine release.
The correlation between wing kinematics and steering muscle activity in the blowfly Calliphora vicina
Authors: Balint Claire N. , Dickinson Michael H. 
Published: April 2021

SUMMARY
Determining how the motor patterns of the nervous system are converted into the mechanical and behavioral output of the body is a central goal in the study of locomotion. In the case of dipteran flight, a population of small steering muscles controls many of the subtle changes in wing kinematics that allow flies to maneuver rapidly. We filmed the wing motion of tethered Calliphora vicina at high speed and simultaneously recorded multi-channel electromyographic signals from some of the prominent steering muscles in order to correlate kinematics with muscle activity. Using this analysis, we found that the timing of each spike in the basalare muscles was strongly correlated with changes in the deviation of the stroke plane during the downstroke. The relationship was non-linear such that the magnitude of the kinematic response to each muscle spike decreased with increasing levels of stroke deviation. This result suggests that downstroke deviation is controlled in part via the mechanical summation of basalare activity. We also found that interactions among the basalares and muscles III2–III4 determine the maximum forward amplitude of the wingstroke. In addition, activity in muscle I1 appears to participate in a wingbeat gearing mechanism, as previously proposed. Using these results, we have been able to correlate changes in wing kinematics with alteration in the spike rate, firing phase and combinatorial activity of identified steering muscles.
Longitudinal variation in muscle protein expression and contraction kinetics of largemouth bass axial muscle
Authors: Thys Tierney M. , Blank Jason M. , Coughlin David J. , Schachat Fred 
Published: April 2021

SUMMARYThe present study investigates muscle protein expression in largemouth bass Micropterus salmoides through intra- and intermyomeric comparisons of white muscle. Using denaturing SDS-polyacrylamide gel electrophoresis, muscle protein expression in the arm and cone regions of sequential myomeres was compared for three bass. Low percentage (4.75 %) polyacrylamide-SDS gels and cyanogen bromide (CNBr) peptide mapping revealed no obvious intramyomeric differences between the myosin heavy chains of the arm and cone regions. Electrophoresis of myofibrils and muscle homogenates on higher percentage gels also failed to demonstrate any significant differences between arm and cone regions in either the myosin light chains or any of the major insoluble and soluble contractile proteins. Two differences were discovered intermyomerically: (i) the ratio of two troponin T isoforms changed from head to tail and (ii) caudal muscle had a lower total parvalbumin content than rostral muscle. Since troponin T and parvalbumin have been implicated in the regulation of skeletal muscle kinetics, longitudinal variation in muscle contraction kinetics was predicted. Subsequent experiments revealed that bass rostral white muscle showed faster rates of activation and relaxation than more caudal muscle, as has been observed in white muscle of other fish species. Rostral–caudal variations in white muscle protein composition and contractile properties are predicted to affect patterns of power production during fast, unsteady swimming.
Selective ablation of antennular sensilla on the Caribbean spiny lobsterPanulirus argussuggests that dual antennular chemosensory pathways mediate odorant activation of searching and localization of food
Authors: Steullet Pascal , Dudar Omar , Flavus Tanya , Zhou Min , Derby Charles D. 
Published: April 2021

SUMMARYIn spiny lobsters and other decapod crustaceans, odorant-mediated searching behavior patterns are driven primarily by chemosensory neurons in the antennules. Two groups of antennular chemosensory neurons can be distinguished on the basis of the sensilla that they innervate and their central projections: those that innervate the aesthetasc sensilla on the lateral flagella and project into the glomerularly organized olfactory lobes, and those that innervate other (i.e. non-aesthetasc) sensilla on both lateral and medial flagella and project into the stratified and non-glomerularly organized lateral antennular neuropils. By ablating different groups of antennular sensory neurons or sensilla, we examined the role of aesthetasc and non-aesthetasc chemosensory neurons in regulating local searching behavior of Caribbean spiny lobsters, Panulirus argus, for food (squid) in a low-flow environment. The results show that odorant-mediated activation of searching and localization of food under these conditions requires only a subset of functional antennular chemosensory neurons, since neither aesthetasc chemosensory neurons nor non-aesthetasc chemosensory neurons are by themselves necessary for these types of behavior. However, ablation of aesthetasc chemosensory neurons together with subsets of non-aesthetasc chemosensory neurons from either the medial or lateral flagella impairs the ability of lobsters to locate the food. This reveals a large degree of functional redundancy but also some complementary functions between aesthetasc and non-aesthetasc chemosensory neurons, and hence between these dual antennular chemosensory pathways, in odorant-mediated searching behavior of lobsters under these conditions.
Selection for high voluntary wheel-running increases speed and intermittency in house mice (Mus domesticus)
Authors: Girard I. , McAleer M. W. , Rhodes J. S. , Garland T. 
Published: April 2021

SUMMARY
In nature, many animals use intermittent rather than continuous locomotion. In laboratory studies, intermittent exercise regimens have been shown to increase endurance compared with continuous exercise. We hypothesized that increased intermittency has evolved in lines of house mice (Mus domesticus) that have been selectively bred for high voluntary wheel-running (wheel diameter 1.12 m) activity. After 23 generations, female mice from four replicate selection lines ran 2.7 times more revolutions per day than individuals from four random-bred control lines. To measure instantaneous running speeds and to quantify intermittency, we videotaped mice (N=41) during a 5-min period of peak activity on night 6 of a 6-day exposure to wheels. Compared with controls (20 revs min–1 while actually running), selection-line females (41 revs min–1) ran significantly faster. These instantaneous speeds closely matched the computer-recorded speeds over the same 5-min period. Selection-line females also ran more intermittently, with shorter (10.0 s bout–1) and more frequent (7.8 bouts min–1) bouts than controls (16.8 s bout–1, 3.4 bouts min–1). Inter-bout pauses were also significantly shorter in selection-line (2.7 s) than in control-line (7.4 s) females. We hypothesize that intermittency of locomotion is a key feature allowing the increased wheel-running performance at high running speeds in selection-line mice.
Similitude in the Cardiovascular System of Mammals
Authors: Dawson Thomas H. 
Published: April 2021

ABSTRACT
Scaling laws governing the cardiovascular system of mammals are discussed in the present review in a manner emphasizing their experimental basis. Specific attention is given to the well-known experimental laws requiring the rate of oxygen consumption and the heart rate of mammals to vary with body mass raised to the powers 3/4 and −1/4, respectively. This review involves reconsideration and further discussion of the previous work of the writer in which these and other scaling relationships were developed from fundamental considerations. The predicted scaling laws remain unchanged from the earlier work, but alternative assumptions leading to the laws are used so as to provide additional insight. The scaling laws are shown to have their origin in the basic design of the cardiovascular system and in the basic processes involved in its working. Modification of the design assumptions of the system to account for known differences in the relative heart masses of mammals and birds is shown to lead to the scaling laws for rate of oxygen consumption and heart rate of birds.
The Influence of Thermal Acclimation on Power Production During Swimming I. In Vivo Stimulation and Length Change Pattern of Scup Red Muscle
Authors: Rome Lawrence C. , Swank Douglas M. 
Published: April 2021

ABSTRACT
Ectothermal animals are able to locomote in a kinematically similar manner over a wide range of temperatures. It has long been recognized that there can be a significant reduction in the power output of muscle during swimming at low temperatures because of the reduced steady-state (i.e. constant activation and shortening velocity) power-generating capabilities of muscle. However, an additional reduction in power involves the interplay between the non-steady-state contractile properties of the muscles (i.e. the rates of activation and relaxation) and the in vivo stimulation and length change pattern the muscle undergoes during locomotion. In particular, it has been found that isolated scup (Stenotomus chrysops) red muscle working under in vivo stimulus and length change conditions (measured in warm-acclimated scup swimming at low temperatures) generates very little power for swimming. Even though the relaxation of the muscle has slowed greatly, warm-acclimated fish swim with the same tail-beat frequencies and the same stimulus duty cycles at cold temperatures, thereby not affording the slow-relaxing muscle any extra time to relax.
We hypothesize that considerable improvement in the power output of the red muscle at low temperatures could be achieved if cold acclimation resulted in either a faster muscle relaxation rate or in the muscle being given more time to relax (e.g. by shortening the stimulus duration or reducing the tail-beat frequency). We test these hypotheses in this paper and the accompanying paper.
Scup were acclimated to 10 °C (cold-acclimated) and 20 °C (warm-acclimated) for at least 6 weeks. Electromyograms (EMGs) and high-speed cine films were taken of fish swimming steadily at 10 °C and 20 °C. At 10 °C, we found that, although there were no differences in tail-beat frequency, muscle strain or stimulation phase between acclimation groups, cold-acclimated scup had EMG duty cycles approximately 20 % shorter than warm-acclimated scup. In contrast at 20 °C, there was no difference between acclimation groups in EMG duty cycle, nor in any other muscle length change or stimulation parameter.
Thus, in response to cold acclimation, there appears to be a reduction in EMG duty cycle at low swimming temperatures that is probably due to an alteration in the operation of the pattern generator. This novel acclimation probably improves muscle power output at low temperatures compared with that of warm-acclimated fish, an expectation we test in the accompanying paper using the work-loop technique.