bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth performance of 6th generation KUB-2 chicken
Authors: Komarudin K , Sartika T , Pratiwi N , Kostaman T 
Published: November 2021

Abstract
KUB-2 chicken is an improved local chicken originally from KUB-1 chicken. KUB-2 has been assembled in the Indonesian Research Institute for Animal Production (IRIAP), and it has two sub-populations, namely KUB-2 Balai and KUB-2 kk. KUB-2 has dual purpose functions, for either meat or egg production. For farmers who raise a local chicken to yield meat, growth is an important trait. The study had the objective to evaluate the growth performance of KUB-2 chicken. About 2,540 6th generation KUB-2 chickens, consisting of 1,240 KUB-2 Balai and 1,300 KUB-2 kk were used in the study. Data were analyzed using a t-test. The average ten-week body weight of male and female KUB-2 kk was statistically higher than KUB-2 Balai (P<0.05). The ten-week body weight of KUB-2 Balai and KUB-2 kk chickens were 1,045.91 g and 1,211.80 g for males, and 832.74 g and 956.02 g for females. The feed conversion was 2.81 for KUB-2 Balai and 2.83 for KUB-2 kk chicken. For meat production, KUB-2 kk seemed more profitable compared to KUB-2 Balai since it had higher body weight and similar feed conversion. It also could be yielded at shorter age than KUB-2 Balai for the same body weight.
Heat shock transformation and expression of the plasmid containing cytolethal distending toxin of Campylobacter fetus subsp venerealis in Escherichia coli
Authors: Herlina N , Yanthi N D , Pratiwi R D , Dewi K S , Setiyoningrum F , Priyoatmojo D , Manggung R D P 
Published: November 2021

Abstract
The cytolethal distending toxins (cdt) is a multi-subunit toxin consisted of three subunit encoded cdtA, cdtB and cdtC. The cdt played an important role as a virulence factor of Campylobacter infection, including C. fetus subsp venerealis. The cdtA which responsible for binding the cdt to cell membrane, was cloned in plasmid expression and inserted into bacterial cells of Escherichia coli BL21(DE3). The research was conducted to evaluate the transformation using the heat shock method of a plasmid containing cdtA3 gene and the protein expression induced by various concentration of IPTG. Transformation was done using the heat shock method at 42oC for 90 second. Evaluation of the transformation was observed on the presence of E. coli BL21(DE3) colonies on Luria Bertani agar containing Ampicillin antibiotic with 100 µg/mL dosage. The recombinant protein was expressed using IPTG-induction with various concentration (0.1mM, 0.25mM, 0.5mM, 0.75mM and 1 mM). The result showed that the transformation and IPTG-induction 0.1 mM produced higher concentration of protein than other concentration applied. The protein characterization was observed with SDS PAGE and cdtA3 protein was detected on 23,4 kDa.
The quality of Etawah crossbreed sperm after sexing with different combination of Bovine Serum Albumin concentrations
Authors: Rasad S D , Solihati N , Winangun K 
Published: November 2021

Abstract
This research aimed to determine the quality of Etawah crossbreed sperm after sexing with different combinantion of Bovine Serum Albumin (BSA) concentrations. The parameter of this research were motility, viability, intact plasma membrane (IPM) and intact acrosome cap (IAC) (%). The Completely randomized design (CRD) was applied in this experiment involving 4 treatments of the combination of BSA concentrations (T1=3%:6%, T2 = 4%:8%, T3=5%:10%, T4=6%:12% at upper and lower fraction) and each treatments was repeated 5 times at post chilled and post sexing. Data were analyzed by using analysis of variance (ANOVA), followed by Duncan Multiple Range Test. The result showed that thecombination of BSA concentrations affected (P<0.05) motility, viability, IPM and IAC. The highest value of sperm quality in upper and lower fraction was obtained from the combination BSA of 5%:10% (motility 78.60 ± 2.61% and 73.80 ± 2.49%; viability 282 ± 14.30 and 252.8 ± 12.97 hours; IPM-value 79.60 ± 1.98% and 74.70 ± 1.82% and IAC 81.00 ± 1.46% and 76.90 ± 1.29%). Based on the results it can be concluded that the quality of Etawah crossbreed sperm after sexing is affected by the combination of BSA concentrations.
Proximate analysis of Dadih from Kapau, Agam Regency, West Sumatera, Indonesia
Authors: Putri M T , Juliyarsi I , Roza E , Purwati E 
Published: November 2021

Abstract
Dadih is a traditional food from buffalo milk fermentation in West Sumatera, Indonesia. which is fermented for 24-48 hours at room temperature. This study aimed to determine the proximate analysis of Dadih from Kapau, Agam Regency West Sumatera, Indonesia. The measured variables were protein content, fat content, water content and, pH. The research method was a descriptive method and analysis in the laboratory. The sample used was taken in Kapau Agam Regency, West Sumatera, Indonesia. The results showed that the nutrition composition of Dadih is: protein content ranged from 7.96%, fat content ranged from 5.53%, water content ranged from 73.55%, pH ranged from 5.16. The conclusion is that Dadih has good nutritional quality.
Future challenge on probiotics uses from fermented milk on the endocrine disorder in human
Authors: Fitria N , Sari Y O , Putry A R , Putrizeti F , Sukma A 
Published: November 2021

Abstract
More than fifty different probiotics have been identified with different structures and modes of action. This study aimed to offer an overview of the evidence on the clinical microbiology activity of the probiotics group derived from fermented milk. We conducted a framework for reporting probiotics activity using the MICRO (Microbiology Investigation Criteria for Reporting Objectively) checklist. Two electronic databases (Pubmed and EMBASE) were used to conduct this study. The initial search discovered 22 references (Pubmed 8 and EMBASE 16). We included human study, clinical trial study, English written, and full articles in this review. Of all these original articles, only six articles were included in the review. These included articles reported the testing phase on the pre-analytical and analytical phases. This review found that probiotics such as Lactobacillus acidophilus La-5 and Bifidobacterium animalis subsp lactis BB-12 are beneficial in humans’ endocrine disorder therapy. This activity includes maintaining serum insulin levels in pregnant women and reducing weight in healthy obese men and women. Thus, probiotics, with their several features, may advance their candidacy as therapeutic agents. However, there is more effort to do. For example, finishing the analytical phase, especially on quality assurance and avoiding bias.
Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
Authors: Chai M H , Sukiman1 M Z , Chan Y F , Liew Y W , Lai L Z H , Mohamad N M , Ariffin S M Z , Ghazali M F 
Published: November 2021

Abstract
The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular characteristics of Staphylococcus aureus (S. aureus) in swine from selected swine farms in Peninsular Malaysia. The oral and nasal swabs of 200 live swine were collected. Screening and isolation of S. aureus isolates were carried out using phenotypic identification techniques. The identity of the bacteria isolates was confirmed by detection of nuc gene (S. aureus) and mecA gene (MRSA). Antibiogram of S. aureus against 19 antibiotics was developed using Kirby-Bauer test. Molecular detections of antimicrobial resistance and virulence genes as well spa typing of S. aureus was carried out. 25% of swine were carrying S. aureus with 9% being MRSA carriers. S. aureus and MRSA have shown high resistance against clindamycin, tetracycline, chloramphenicol, oxacillin, penicillin, erythromycin, cefoxitin and amoxicillin/clavulanate. Antimicrobial resistance and virulence genes, including tetK, tetL, tetM, ermA, ermB, ermC, msrA and scn genes were detected. 93% of S. aureus were multiple-drug resistant (MDR), suggesting the emergence of MDR S. aureus in swine in Malaysia.
Edamame soybean protein concentrate as a source of amino acid nutrition for poultry
Authors: Hertamawati R T , Nurkholis  , Rahmasari R 
Published: November 2021

Abstract
This study aims to determine the opportunity and characteristics of edamame soybean protein concentrate (eSPC) as a source of amino acid. In this study, edamame soybean protein concentrate (eSPC) was manufactured using various methods of manufacturing solvent-based materials. This experiment consisted of two different methods, namely washing with acid and ethanol solvent. The results of manufacturing eSPC were analyzed for nutrients, especially the protein and amino acids contents. From the presentation and description of data, the best results of edamame soybean processing into protein concentrate (eSPC) was obtained in the acid method with a protein content of 48.83% and a total of 36.66% amino acids. The highest content of lysine was obtained at eSPC processing using 3.77% of acid, while methionine highest was with approximately 1.12%. These results showed that the eSPC is well used as a source of lysine and methionine in poultry feed.
Rumen degradation properties of tropical legumes feed under in sacco studies
Authors: Rahmat S F I , Permana I G , Despal  
Published: November 2021

Abstract
Degradation properties of protein feed sources such as tropical legumes is very important to match with microbial protein synthesis and by pass protein requirements in dairy cattle ration formulation. However, the information is still limited. This study aims to determine the degradability of dry matter (DMD) and organic matter (OMD) from several legumes in Indonesia using in sacco method. There are 11 types of tropical legumes that are commonly used in dairy cattle ration have been studied, namely acacia, alfalfa, pterocarpus, gliricidia, indigofera, calliandra, butterfly leaf, leucaena, albizia, tamarind, and sesbania. The dried forage mesh samples were put into nylon bags and incubated in the rumen of two fistulated Friesian Holstein bulls for 0, 3, 6, 9, 12, 15, 24, 48, and 72 hours. Parameter observed were degradability of dry matter (DMD), and organic matter (OMD), kinetic parameters, and effective degradation. The data were analysed using descriptive statistic and regression from SAS University software. The result that sesbania and indigofera grouped into highly degradable forage (degradability > 80%), while acacia, albizia, calliandra grouped into low degradable forage (degradability < 50%). From this study it can be concluded that each type of legume has different degradability characteristics using in sacco method.
The effect of zinc supplementation in different types of feed on the performance and health status of IPB-D2 chickens
Authors: Khairunnisa Siti , Sumiati  , Sumantri Cece , Winarsih Wiwin 
Published: November 2021

Abstract
This study aimed to evaluate the effect of zinc supplementation in various diets on the performance and health status of IPB-D2 chickens. Subsequently, about 180 one-week-old IPB-D2 chickens were used in the study and were fed the treatment diets for 12 weeks. Also, this study employed a completely randomised factorial design with six treatments and three replications of ten birds each. These treatment diets included A1, which contained nutrients based on SNI (2013) without Zn supplementation, A2 which is a combination of A1 and 60 ppm of Zinc, B1 which contained nutrients 5% higher than SNI without Zn supplementation, B2 which is a combination of B1 and 60 ppm Zinc, C1 which contains nutrients 10% higher than SNI without Zn supplementation, and lastly, C2 which is a combination of C1 and 60 ppm of Zinc. Furthermore, the data were analysed using ANOVA, and the parameters observed were the body weight, body weight gain, feed consumption, feed conversion ratio, blood profile and the villi surface area of the jejunum. Consequently, the results showed that C2 significantly (P<0.05) increased the body weight and body weight gain when fed to the finisher and grower chickens, respectively. More so, when the finisher chickens were fed the 10% SNI diet, there was an increased body weight gain and also a significantly (P<0.05) decreased feed conversion ratio. Furthermore, the addition of 60 ppm zinc increased haemoglobin significantly (P<0.05), whereas C2 decreased leukocytes significantly (P<0.05) in chicken blood. However, the treatments had no effect on feed consumption in the villi surface area of the jejunum. Therefore, the study concluded that supplementing the IPB-D2 chickens with 60 ppm zinc in a 10% SNI diet resulted in the best performance and health status.
Mangrove (Avicennia marina) leaves as an alternative feed resources for ruminants
Authors: Sari R W W , Jamarun N , Suyitman  , Khasrad  , Yanti G 
Published: November 2021

Abstract
The aim of this research was to get the best treatment for preserving of mangrove (Avicennia marina) leaves as an alternative feed resouces for ruminants. This research used experimental method using a completely randomized design (CRD) with 2 treatments and 5 replications for each treatment. The treatments are: P1 (Mangrove leaves silage) and P2 (Mangrove leaves hay). The variables observed in the in-vitro experiment were in-vitro rument fluid characteristics (pH, NH3, VFA), total gas production and methane gas production. The results of the in-vitro research showed that the P2 treatment (mangrove hay) produced : pH 6,67, VFA 83 Mm, NH3 5,44 mg/100 ml, total production gas for 48 hours 99,7 ml/hour, and methane gas production for 48 hours 65,05 ml/gr DM. From this research can be concluded that the best treatment for preservation of mangrove leaves (Avicennia marina) was the hay treatment based on the total gas and methane gas production. It can be concluded that the hay mangrove leaves (Avicennia marina) can be used as an alternative resource feed for ruminant animals.
The study of local beef market structure in Jakarta, Indonesia
Authors: Agustiar R , Triatmojo A , Guntoro B 
Published: November 2021

Abstract
This study was implemented to determine benefit distribution from the value chain and analyze Jakarta’s local beef market’s price determination processes. The research method was a survey, and a purposive sampling technique was used to select key informants. A structured questionnaire was designed to collect the data supplemented with focus group discussion. Data were analyzed using descriptive quantitative statistics. The descriptive statistics revealed a market structure based on the elements to have many consumers and suppliers with ex-imported cattle (98 percent) and local cattle from East Nusa Tenggara and East Java (2 percent) fulfilled the demand in a year. Middlemen in the supply chain have more significant benefits than producers (feedlots and farmers) and consumers. The implication of the study is developing a business model for modern retail or butchering-based meat shops that increases the added value of local meat and benchmarking of a long beef chain efficiency at post-cutting (downstream). The development of a modern retail business model is expected to drive the shifting system sales of beef from a commodity to a product basis.
Performance analysis of milk processing units to support competitive improvement of the milk processing industry in Padang Panjang City, West Sumatera Province
Authors: Wati Rahmi , Tan Firwan , Andri  , Suresti Amna , Edwin Tevina 
Published: November 2021

Abstract
Dairy cattle productivity has plateaued; average milk production ranges between 8 and 12 liters per day, on a scale of 2-3 maintenance per family breeder breeding. Furthermore, small farms account for 90% of milk production. Permata Ibu’s Milk Processing Unit (UPS) in Padang Panjang is one of the business units that processes milk into other derivative products. It produces processed milk pasteurization, yogurt, and other products. The focus of this research was to analyze UPS Permata Ibu’s processing performance by calculating the amount of added value and profitability. As measured by the value-added method of performance measurement, the processing of fresh milk into pasteurized milk and yogurt at Permata Ibu milk processing unit generates a variety of added values. Pasteurized milk has better processing performance than yogurt processing, as evidenced by its high added value compared to other processed products, with added value ratios of 22,45 percent and 12,50 percent for pasteurized milk and yogurt, respectively. Based on profitability analysis, it is known that pasteurized milk is still better than yogurt because it has a higher profitability value of 30,30% compared to yogurt, which is only 6,50%. Pasteurized milk processing results in higher profitability and added value than yogurt.
The use of N HNO3 to determine copper and zinc levels in heavy-metal polluted tropical soils
Authors: Salam A K , Milanti M , Silva G , Rachman F , Santa I M T D , Rizki D O , Novpriansyah H , Sarno S 
Published: November 2021

Abstract
This study compared N HNO3 to other methods to determine plant available heavy metals in heavy-metal polluted soils. Soil samples were obtained from an experimental field treated with industrial waste after 22 years of the amendment and employed to conduct the comparative and correlation study. Soil samples were analyzed for Cu using various methods, planted in a glass house with several plants, and analyzed for soil and plant Cu and Zn. The relative strength of the chemical extractants followed the order of N HNO3 ≈ N HCl > Buffered DTPA ≈ Unbuffered DTPA > M CaCl2 ≈ N NH4OAc pH 7. A high correlation was observed for soil extracted Cu by M CaCl2 or N NH4OAc pH 7 or N HCl vs. Buffered DTPA and N HNO3 or N NH4OAc pH 7 vs. N HCl. High correlations of plant and soil Cu extracted by N HNO3 were shown by caisim, water spinach, land spinach, and corn, while plant and soil Zn were shown by caisim, water spinach, land spinach, and lettuce.
Study of agile governance on strengthening reforestation program in West Java Indonesia
Authors: Nur M I , Juana T , Zain F C , Astuti D R 
Published: November 2021

Abstract
West Java is one of the provinces in Indonesia that possesses the most critical land area, approximately 714,000 of 911,000 hectares (ha) in total. So that to deal with that severe issue, the Forestry Office of West Java has established an eminent program named “Gerakan Tanam dan Rawat 50 Juta Pohon (The Movement of 50 Million Trees Planting and Caring)”. This research aims to analyze the reforestation program in West Java Indonesia viewed from an agile governance perspective. The study employed the qualitative approach with the descriptive case study method. In addition, in-depth interviews and literature studies were utilized in collecting the data. Moreover, to ensure validity, this study uses the triangulation technique. This study showed that the Planting and Preserving 50 million Trees Program carried by the stakeholders has applied agile governance. The government has made the program in collaboration with various media and technology. However, several activities must be fulfilled more appropriately among multiple parties, especially the government, which is the leading sector.
Co-design as a strategy to improve ecological awareness of communities
Authors: Setiawan A 
Published: November 2021

Abstract
One strategy of communities to easily embrace ecological awareness is by involving directly in the improvement of their environmental quality. This paper discusses the case study research of the co-design process of the neighborhood regeneration project in Kampung Tongkol at the Ciliwung riverbank, Jakarta. This project is carried out collaboratively involving residents of the area and facilitated by ASF (Architecture Sans Frontières) Indonesia. The main important object of this regeneration project is the construction of a self-supporting sample house. This house aims as an ideal model which another neighborhood can replicate. Residents are directly involved in the organizing, the design process, to the construction stage. The primary purpose of regeneration is to improve the quality of the residential environment. The principles of sustainable design are employed as the main guidance from the beginning of the process. This study concludes that the final achievement of this project not only results in a higher quality environment but also raises the ecological awareness of the residents of the neighborhood.
The growth and leaf chlorophyll in black rice (Oryza sativa L. Indica) plants induced by gamma rays as responses to the drought stress condition
Authors: Purwanto E , Ula A M , Parjanto  
Published: November 2021

Abstract
The aims of this study to determine the growth and leaf chlorophyll content in black rice (Oryza sativa L. Indica) which were induced by gamma rays as responses to the drought stress conditions. This study was carried out on July–November 2020, at the experimental field (screenhouse), Medicinae Street, UNS Perum, Jati Village, Jaten District, Karanganyar Regency. Meanwhile, the chlorophyll content test was carried out at Plant Tissue Culture and Biotechnology Laboratory, Faculty of Agriculture, Universitas Sebelas Maret. The study was a field trial research design without replication. The research treatments were 24 genotypes as follows: 10 genotypes of 100 gray gamma-ray irradiation with 2 control genotypes; and 10 genotypes of 200 gray gamma-irradiation with 2 control genotypes. The results showed that the plant height was lower in plants with irradiation compared to the Control-01 plant. The crown-root ratio showed irradiated plants had higher average results than control plants. The leaf chlorophyll of 100 gray irradiated plants showed higher results than control; while 200 gray irradiated plants showed similar results.
A comparative study of Jarak Towo cassava farming in the mountainous areas of Karanganyar Regency in supporting the availability of raw materials for food processing industry
Authors: Irianto H , Mujiyo  , Qonita A , Riptanti E W , Ningsih H 
Published: November 2021

Abstract
Cassava is a commodity that is commonly marginalized. However, jarak towo cassava is exceptional as this variety has a large market potential with a more competitive price. However, only a few farmers are aware of this business potential. This study aimed to conduct a comparative study of the cropping patterns of jarak towo cassava to investigate the farmers’ income or profit more thoroughly. This study was carried out in three sub-districts, including Jatiyoso, Tawangmangu, and Ngargoyoso, the respondents were taken using snowball sampling, with 20 respondents from each sub-district. The results showed that farmers in Ngargoyoso Sub-district spent the highest total cost. Meanwhile, farmers in Jatiyoso Sub-district received the highest income and profit. The highest efficiency was shown by farmers in Jatiyoso, while the lowest efficiency was performed by farmers in Tawangmangu. These findings depict how farmers develop jarak towo cassava farming in meeting the market demand.