bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long noncoding RNA MCM3AP‐AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR‐193a‐5p/SENP1
Authors: Zhou Mingyue , Bian Zehua , Liu Bingxin , Zhang Yi , Cao Yulin , Cui Kaisa , Sun Shengbai , Li Jiuming , Zhang Jia , Wang Xue , Li Chaoqun , Yao Surui , Yin Yuan , Fei Bojian , Huang Zhaohui 
Published: March 2021

AbstractBackgroundAccumulating evidences have shown that long noncoding RNAs (lncRNAs) play key roles in many diseases, including cancer. Several studies reported that MCM3AP antisense RNA 1 (MCM3AP‐AS1) was associated with the tumorigenesis and progression. However, the specific function and mechanism of MCM3AP‐AS1 in colorectal cancer (CRC) have not been fully understood.MethodsThe expression of MCM3AP‐AS1 was detected by quantitative reverse transcription PCR (RT‐qPCR) in CRC tissues and matched noncancerous tissues (NCTs). CCK‐8 assay, colony formation assay, transwell assay, xenograft and lung metastasis mouse models were used to examine the tumor‐promoting function of MCM3AP‐AS1 in vitro and in vivo. The binding relationship between MCM3AP‐AS1, miR‐193a‐5p and sentrin‐specific peptidase 1 (SENP1) were screened and identified by databases, RT‐qPCR, dual luciferase reporter assay and western blot.ResultsIn the present study, we got that the expression of MCM3AP‐AS1 was higher in CRC tissues than in paired NCTs, and increased MCM3AP‐AS1 expression was associated with adverse outcomes in CRC patients. Functional experiments in vitro revealed that silencing of MCM3AP‐AS1 could inhibit the proliferation, colony formation, migratory, and invasive abilities of CRC cells. The mouse models of xenograft and lung metastasis further confirmed that in vivo silencing MCM3AP‐AS1 could significantly inhibit the growth and metastasis of CRC. Further mechanism studies indicated that MCM3AP‐AS1 could sponge miR‐193a‐5p and inhibit the activity of it. What is more, SENP1 was proved to be a novel target of miR‐193a‐5p and could be upregulated by MCM3AP‐AS1. At last, we observed that SENP1 overexpression in CRC tissues was closely related to unfavorable prognosis.ConclusionTaken together, we identified in CRC the MCM3AP‐AS1/miR‐193a‐5p/SENP1 regulatory axis, which affords a therapeutic possibility for CRC.
Non‐Aggregating Amylin Fragments as an Inhibitors of the Aggregation Process of Susceptible to Aggregation Fragments 18–22, 23–27, and 33–37 of Hormone
Authors: Fraczyk Andrzej , Janczewski Lukasz , Wasko Joanna , Rozniakowski Kamil , Galecki Krystian , Kaminski Zbigniew J. , Kolesinska Beata 
Published: March 2021

AbstractAmylin aggregation is one of the factors in the development of diabetes mellitus, which is classified as a civilization disease. The aim of this research was to find whether non‐aggregating fragments 1–7, 8–12, 13–17 and 28–32 of amylin would inhibit the aggregation of the amyloidogenic cores 18–22, 23–27, 33–37 of hormone. In the study of the inhibitory potential of non‐aggregating amylin fragments, a set of independent methods were used to study aggregation properties (spectroscopic and fluorescence studies with the use of indicators, microscopic studies, circular dichroism studies) and the method of prediction of aggregation properties. The performed research allowed to select the cyclic fragment (1–7) H‐KCNTATC‐OH with disulfide bond as an inhibitor capable of inhibiting the aggregation of all amyloidogenic cores 18–22, 23–27, 33–37 of the hormone. Additionally, it was found that this peptide inhibits insulin hot spot aggregation, which may indicate its universal utility in inhibiting the process of aggregation of hormones regulating carbohydrate metabolism directly related to the development of diabetes. Research on the possibility of the extensive use of the cyclic fragment (1–7) of H‐KCNTATC‐OH as a peptide inhibitor of the polypeptide/protein aggregation process is ongoing.
Familiar del3p syndrome: The uncertainty of the prognosis. A case report
Authors: Martins Márcia , Arantes Regina , Botelho Pedro , Souto Marta , Moutinho Osvaldo , Pinto Leite Rosário 
Published: March 2021

AbstractThe 3p deletion syndrome is an unusual condition. The few cases described are mainly de novo. We described a familial case detected in a prenatal diagnosis. Three members of the family had the 3p26.3‐p26.1 deletion; however, only the son presented clinical features.
Ionic Liquids‐Assisted Highly Luminescent Copper Nanoclusters with Triangle Supramolecular Nanostructures
Authors: Han Bingyan , Yan Qin , Xin Ze , Yan Qifang , Jiang Jingmei 
Published: March 2021

Main observation and conclusionA novel and facile approach towards highly luminescent CuNCs@MMI (2‐mercapto‐1‐methylimidazole) has been developed within 30 s with the assistance of ionic liquids (ILs)—[Bmim]BF4. FT‐IR spectra, Zeta potential, TEM and SEM images showed that the as‐prepared CuNCs@MMI within [Bmim]BF4 could assemble into well‐ordered triangle supramolecular nanostructures via electrostatic interaction. In addition, pH‐responsion of CuNCs@MMI in photoluminescence intensity indicated the great potential for pH sensing applications.
Enzymatic Synthesis of a Diastereomer of Neoabyssomicin Derivative Using the Diels‐Alderase AbyU
Authors: Ding Wenjuan , Chi Changbiao , Wei Xiaoyi , Sun Changli , Tu Jiajia , Ma Ming , Li Qinglian , Ju Jianhua 
Published: March 2021

Main observation and conclusionThe enzyme AbyU catalyses a Diels‐Alder (DA) reaction during abyssomicin C biosynthesis. In this study, AbyU is shown to convert the native substrate of another Diels‐Alderase (DAase), AbmU, to a new abyssomicin derivative, abyssomicin 7. Abyssomicin 7 is a diastereomer of the AbmU‐derived, abyssomicin 6. Using structural analyses and site‐directed mutagenesis, we unveil the molecular basis for production of abyssomicin 7, instead of abyssomicin 6, within the AbyU active site.
Hydroxypyridinethione Inhibitors of Human Insulin‐Degrading Enzyme
Authors: Adamek Rebecca N. , Suire Caitlin N. , Stokes Ryjul W. , Brizuela Monica K. , Cohen Seth M. , Leissring Malcolm A. 
Published: March 2021

AbstractInsulin‐degrading enzyme (IDE) is a human mononuclear Zn2+‐dependent metalloenzyme that is widely regarded as the primary peptidase responsible for insulin degradation. Despite its name, IDE is also critically involved in the hydrolysis of several other disparate peptide hormones, including glucagon, amylin, and the amyloid β‐protein. As such, the study of IDE inhibition is highly relevant to deciphering the role of IDE in conditions such as type‐2 diabetes mellitus and Alzheimer disease. There have been few reported IDE inhibitors, and of these, inhibitors that directly target the active‐site Zn2+ ion have yet to be fully explored. In an effort to discover new, zinc‐targeting inhibitors of IDE, a library of ∼350 metal‐binding pharmacophores was screened against IDE, resulting in the identification of 1‐hydroxypyridine‐2‐thione (1,2‐HOPTO) as an effective Zn2+‐binding scaffold. Screening a focused library of HOPTO compounds identified 3‐sulfonamide derivatives of 1,2‐HOPTO as inhibitors of IDE (Ki values of ∼50 μM). Further structure‐activity relationship studies yielded several thiophene‐sulfonamide HOPTO derivatives with good, broad‐spectrum activity against IDE that have the potential to be useful pharmacological tools for future studies of IDE.
Insights into coronavirus immunity taught by the murine coronavirus
Authors: Grabherr Sarah , Ludewig Burkhard , Pikor Natalia Barbara 
Published: March 2021

AbstractCoronaviruses (CoVs) represent enveloped, ss RNA viruses with the ability to infect a range of vertebrates causing mainly lung, CNS, enteric, and hepatic disease. While the infection with human CoV is commonly associated with mild respiratory symptoms, the emergence of SARS‐CoV, MERS‐CoV, and SARS‐CoV‐2 highlights the potential for CoVs to cause severe respiratory and systemic disease. The devastating global health burden caused by SARS‐CoV‐2 has spawned countless studies seeking clinical correlates of disease severity and host susceptibility factors, revealing a complex network of antiviral immune circuits. The mouse hepatitis virus (MHV) is, like SARS‐CoV‐2, a beta‐CoV and is endemic in wild mice. Laboratory MHV strains have been extensively studied to reveal coronavirus virulence factors and elucidate host mechanisms of antiviral immunity. These are reviewed here with the aim to identify translational insights for SARS‐CoV‐2 learned from murine CoVs.
Tris(pentafluoroethyl)difluorophosphorane and N‐Heterocyclic Carbenes: Adduct Formation and Frustrated Lewis Pair Reactivity
Authors: Föhrenbacher Steffen A. , Zeh Vivien , Krahfuss Mirjam J. , Ignat'ev Nikolai V. , Finze Maik , Radius Udo 
Published: March 2021

AbstractThe synthesis and characterization of Lewis acid/base adducts between tris(pentafluoroethyl)difluorophosphorane PF2(C2F5)3 and selected N‐heterocyclic carbenes (NHCs) R2Im (1,3‐di‐organyl‐imidazolin‐2‐ylidene) and phosphines are reported. For NHCs with small alkyl substituents at nitrogen (R=Me, nPr, iPr) the adducts NHC ⋅ PF2(C2F5)3 (2 a–h) were isolated. The reaction with the sterically more demanding NHCs Dipp2Im (1,3‐bis‐(2,6‐di‐iso‐propylphenyl)‐imidazolin‐2‐ylidene) (1 i) and tBu2Im (1,3‐di‐tert‐butyl‐imidazolin‐2‐ylidene) (1 j) afforded the aNHC adducts 3 i and 3 j (a denotes “abnormal” NHC coordination via a backbone carbon atom). The use of tBuMeIm (1‐tert‐butyl‐3‐methyl‐imidazolin‐2‐ylidene) (1 m) led to partial decomposition of the NHC and formation of the salt [tBuMeIm−H][MeIm ⋅ PF2(C2F5)3] (4 m). The phosphorane PF2(C2F5)3 forms adducts with PMe3 but does not react with PPh3 or PCy3. The mer‐cis isomer of literature‐known Me3P ⋅ PF2(C2F5)3 (5 a) was structurally characterized. Mixtures of the phosphorane PF2(C2F5)3 and the sterically encumbered NHCs tBu2Im, Dipp2Im, and Dipp2ImH2 (1,3‐bis‐(2,6‐di‐iso‐propylphenyl)‐imidazolidin‐2‐ylidene) (1 k) showed properties of FLPs (Frustrated Lewis Pairs) as these mixtures were able to open the ring of THF (tetrahydrofuran) to yield NHC−(CH2)4O−PF2(C2F5)3 6 i–k. Furthermore, the deprotonation of the weak C−H acids CH3CN, acetone, and ethyl acetate was achieved, which led to the formation of the corresponding imidazolium salts and the phosphates [PF2(C2F5)3(CH2CN)]− (7), [PF2(C2F5)3(OC(=CH2)CH3)]− (8) and [PF2(C2F5)3(CH2CO2Et)]− (9).
A new evolutionary polynomial regression technique to assess the fundamental periods of irregular buildings
Authors: Marasco Sebastiano , Cimellaro Gian Paolo 
Published: March 2021

AbstractThe main seismic design codes propose simplified formulations to evaluate the fundamental period of regular structures based on the total height. Indeed, the fundamental period depends on several parameters directly connected to the mass and stiffness of the structure and on its geometrical characteristics, including also irregularities.This paper proposes a set of mathematical formulations to evaluate the longitudinal and transversal fundamental period of vibration of 3D Reinforced Concrete (RC) frames, which have various vertical and plan irregularities and for different mechanical and geometrical design parameters.Several types of Reinforced Concrete Bare Moment Resisting Frame (RC‐BMRF) buildings have been designed according to the different versions of the Italian codes starting from 1916 to nowadays and then used as case studies. Modal analysis is performed on the entire building dataset to assess the fundamental periods in both longitudinal and transversal directions. Then, cluster analysis is carried out to classify the buildings based on similar design characteristics and construction details. Finally, a robust Evolutionary Polynomial Regression (EPR) technique is used to find the optimal polynomial forms of the natural period. Numerical results show a better performance of the proposed formulation compared with the existing methodologies available in the literature.
A novel macroelement model for the nonlinear analysis of masonry buildings. Part 2: Shear behavior
Authors: Bracchi Stefano , Penna Andrea 
Published: March 2021

AbstractMacroelement models are widely recognized to be particularly suitable to model the nonlinear behavior of masonry buildings. This article, together with the companion article, presents a new macroelement model, able to solve the limitations of the macroelement model currently implemented in the TREMURI computer program. Whereas the companion article deals with the axial and flexural behavior, this article presents the formulation regarding the shear behavior. In the macroelement currently implemented in the TREMURI program, the shear strength and deformability of an element can be captured only if a manual calibration of equivalent material properties and shear deformation parameters is performed before the analysis. On the contrary, the proposed new macroelement is able to predict the cyclic response of a wall failing in shear, considering multiple strength criteria, directly starting from experimentally measured or code prescribed mechanical properties and automatically calculating the equivalent shear parameters. Furthermore, a procedure to calculate the deformability parameters governing the nonlinear shear response starting from experimentally measured or code prescribed parameters is also included. Therefore, calibrations of material properties and shear deformation parameters at the beginning of the analysis are no longer required. The improved model was then implemented in the TREMURI program and its ability of accurately predicting the nonlinear response of masonry structures is verified through the simulation of experimental tests at different scales.
A novel macroelement model for the nonlinear analysis of masonry buildings. Part 1: Axial and flexural behavior
Authors: Bracchi Stefano , Galasco Alessandro , Penna Andrea 
Published: March 2021

AbstractIn the numerical modelling of the nonlinear response of masonry buildings by equivalent‐frame models based on macroelements representative of the in‐plane response of structural members, it is important to correctly capture compressive behavior, lateral stiffness, and strength of the structure. The macroelement model currently implemented in the TREMURI computer program, thanks to the presence of nonlinear interfaces lumped at the element extremities, allows studying the coupled axial and in‐plane bending response, with the inherent limitation of approximating the stiffness associated with at least one of these behaviors. The simplified compressive law does not capture the displacement accumulation during cyclic loads; moreover, second‐order effects are not modelled. The article presents an improved macroelement model, able to simulate the in‐plane cyclic response of masonry walls. The model overcomes some of the limitations of the currently available macroelement. As regards the compressive and flexural behavior, a new compressive law is introduced, together with a methodology to capture the correct flexural stiffness of the panel and to model the second‐order effects. The improved model was then implemented in the TREMURI program and validations of the new features are shown, together with a simulation of an experimental test on a single wall characterized by flexural behavior. A companion article presents the shear formulation and shows the ability of the new macroelement of accurately predicting the nonlinear response of masonry structures at the building scale through the simulation of a larger number of experimental tests.
Anomaly‐based intrusion detection systems: The requirements, methods, measurements, and datasets
Authors: Hajj Suzan , El Sibai Rayane , Bou Abdo Jacques , Demerjian Jacques , Makhoul Abdallah , Guyeux Christophe 
Published: March 2021

AbstractWith the Internet's unprecedented growth and nations' reliance on computer networks, new cyber‐attacks are created every day as means for achieving financial gain, imposing political agendas, and developing cyberwarfare arsenals. Network security is thus acquiring increasing attention among researchers, practitioners, network architects, policy makers, and others. To defend organizations' networks from existing, foreseen, and future threats, intrusion detection systems (IDSs) are becoming a must. Existing surveys on anomaly‐based IDS (AIDS) focus on specific components such as detection mechanisms and lack many others. In contrast to existing surveys, this article covers the full scope needed by researchers and practitioners alike when studying AIDS. The scope ranges from the intrusion detection techniques to attacks forms and passing through the relevant attack features, most‐used datasets, challenges, and potential solutions. This article provides an exhaustive review of IDSs and discusses their requirements and performance metrics in deep. It presents a taxonomy of IDSs based on four criteria: information source, detection strategy, detection mode, and architecture. Then, in‐depth analysis and a comparison of network intrusion detection approaches based on anomaly detection techniques are given. The article also introduces a classification of computer network attacks, along with their different forms and the relevant network traffic features to detect them, as well as a summary of the popular datasets used by the researchers to evaluate the IDSs. Finally, the article highlights several research challenges and the possible solutions to deal with them.
Modified compact fluorescent lamps improve light‐induced off‐season floral stimulation in dragon fruit farming
Authors: Nguyen Quang Thach , Ngo Minh Dung , Truong Thanh Hung , Nguyen Duy Chinh , Nguyen Minh Chau 
Published: March 2021

AbstractEfficient light‐induced floral stimulation plays a key role in energy conservation and maintaining stable productivity during off‐season periods of dragon fruit plants. In this study, we first reported on results of a survey on dragon fruit farmers regarding use of lamps in performing artificially induced flowering process in Vietnam. It was found that the use of incandescent lamp was prevalent in dragon fruit cultivation practices, resulting in heavy electricity consumption, and that low‐power compact fluorescent light (CFL) bulbs were not extensively utilized, possibly due to low floral induction performance of domestic CFL bulbs. Arguing that emission spectra of currently used lamps were not consistent with adsorption spectra of phytochromes, whose transformation is responsible for flowering process of dragon fruit, we then proposed three improved CFL lamps (power capacity of 20 W) having emission spectra focused on red and far‐red regions. New lamp prototypes were tested in 7 field experiments in three different provinces in Vietnam. One improved CFL bulb (treatment 2) performed relatively well in comparison with the incandescent control lamp (60 W) in six out of seven experiments with regard to some growth indicators (e.g., number of floral stems, number of bubs, number of fruits per plant) and fruit yield. Recent success on commercialization of the improved CFL lamp demonstrates the potential of CFL lamps in floral stimulating irradiation of other crops and plants and in alleviating electricity burden in dragon fruit growing areas.
Intensity‐modulated radiation therapy for nasal cavity and paranasal sinus tumors: Experience from a single institute
Authors: Laskar Sarbani Ghosh , Pai Prathamesh , Sinha Shwetabh , Budrukkar Ashwini , Nair Deepa , Swain Monali , Mummudi Naveen , Gupta Tejpal , Murthy Vedang , Agarwal Jai Prakash , Tiwari Neelesh , Thiagrajan Shiva , Chaukar Devendra , Chaturvedi Pankaj , Pantvaidya Gouri , Deshmukh Anuja , Nair Sudhir , Prabhash Kumar , Joshi Amit , Noronha Vanita , Patil Vijay , Menon Nandini , Patil Asawari , Bal Munita , Mittal Neha , Rane Swapnil 
Published: March 2021

AbstractBackgroundTo assess the efficacy of intensity‐modulated radiation therapy (IMRT) for tumors of the nasal cavity and paranasal sinus (PNS) region.Materials and MethodsTwo hundred fourteen patients with tumors of the nasal cavity and PNS region treated with curative intent IMRT between 2007 and 2019 were included in this retrospective analysis.ResultsFifty‐one (24.1%) received definitive RT/CTRT and 163 (75.9%) received adjuvant RT. Most common histology was squamous cell carcinoma (26.1%) followed by adenoid cystic carcinoma (21.5%). The median follow‐up was 43.5 months. The 5‐year local control (LC), event‐free survival (EFS), and overall survival (OS) for the entire cohort was 66.9%, 59%, and 73.9%, respectively. On univariate analysis treatment with nonsurgical modality, T classification and undifferentiated/poorly differentiated histology were associated with inferior 5‐year LC, EFS, and OS. Four patients had late Grade 3/Grade 4 ocular toxicity.ConclusionsIMRT should be the standard of care for tumors of PNS region across all histologies and treatment setting.
Episodic processes in moral decisions: Evidence from medial temporal lobe amnesia
Authors: Verfaellie Mieke , Hunsberger Renee , Keane Margaret M. 
Published: March 2021

AbstractTheoretical accounts of moral decision making imply distinct ways in which episodic memory processes may contribute to judgments about moral dilemmas that entail high conflict between a harmful action and a greater good resulting from such action. Yet, studies examining the status of moral judgment in amnesic patients with medial temporal lobe (MTL) lesions have yielded inconsistent results. To examine whether and how episodic processes contribute to high conflict moral decisions, amnesic patients with MTL damage and control participants were asked to judge the moral acceptability of a harmful action across two conditions that differed in the framing of the moral question. We predicted that personal (but not abstract) framing would engage episodic processes involved in mental simulation, yielding a selective impairment in MTL patients in the personal framing condition. This prediction was not confirmed as neither patients nor controls were influenced by the framing of the moral question. With the exception of a patient whose lesion extended into the amygdala bilaterally, patients were less willing than controls to endorse the utilitarian option, rejecting the harmful action despite its beneficial outcome. They also rated actions as emotionally more intense than did controls. These findings suggest that episodic processes involved in mental simulation are necessary to prospectively evaluate action‐outcome contingencies.
“They Are Doers”: Writing to Advocate With Immigrant Youth in Community‐Based Organizations
Authors: Lee Crystal Chen , Dufresne Kelsey Virginia , Relyea Jackie Eunjung 
Published: March 2021

AbstractOver the course of a year, student authors in the Juntos NC Writing Project participated in the Literary and Community Initiative to write, publish, and share their lived experiences and identities as Latinx immigrants and first‐generation high school students in North Carolina. Throughout the publication process of their collaborative bilingual book titledThe Voices of Our People: Nuestras Verdades, student authors actively engaged in pursuing advocacy and activism in three ways: (1) community space as an intentional space for advocacy, (2) writing as a vehicle for collective advocacy, and (3) publishing and sharing as an opportunity for youth activism. The participants’ words and actions demonstrated how youth in community organizations can use literacy practices to collectively advocate for their community and become activists who write about and vocalize immigrant youth’s strengths and needs.