bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thorpe Turbulence Scaling in Nighttime Convective Surface Layers in the North Indian Ocean
Authors: Kumar B. Praveen , D’Asaro Eric , Sureshkumar N. , Rama Rao E. Pattabhi , Ravichandran M. 
Published: August 2021

AbstractWe use profiles from a Lagrangian float in the north Indian Ocean to explore the usefulness of Thorpe analysis methods to measure vertical scales and dissipation rates in the ocean surface boundary layer. An rms Thorpe length scale LT and an energy dissipation rate εT were computed by resorting the measured density profiles. These are compared to the mixed layer depth (MLD) computed with different density thresholds, the Monin–Obukhov (MO) length LMO computed from the ERA5 reanalysis values of wind stress, and buoyancy flux B0 and dissipation rates ε from historical microstructure data. The Thorpe length scale LT is found to accurately match MLD for small (<0.005 kg m−3) density thresholds, but not for larger thresholds, because these do not detect the warm diurnal layers. We use ξ = LT/|LMO| to classify the boundary layer turbulence during nighttime convection. In our data, 90% of points from the Bay of Bengal (Arabian Sea) satisfy ξ < 1 (1 < ξ <10), indicating that wind forcing is (both wind forcing and convection are) driving the turbulence. Over the measured range of ξ, εT decreases with decreasing ξ, i.e., more wind forcing, while ε increases, clearly showing that ε/εT decreases with increasing ξ. This is explained by a new scaling for ξ ≪ 1, εT = 1.15B0ξ0.5 compared to the historical scaling ε = 0.64B0 + 1.76ξ−1. For ξ ≪ 1 we expect ε = εT. Similar calculations may be possible using routine Argo float and ship data, allowing more detailed global measurements of εT, thereby providing large-scale tests of turbulence scaling in boundary layers.
Blockade of BK channels attenuates chronic visceral hypersensitivity in an IBS-like rat model
Authors: Fan F , Chen Y , Chen Z , Guan L , Ye Z , Tang Y , Chen A , Lin C 
Published: August 2021

Background Visceral hypersensitivity in irritable bowel syndrome (IBS) is still poorly understood, despite that chronic abdominal pain is the most common symptoms in IBS patients. To study effects of BK channels on visceral hypersensitivity in IBS rats and the underlying mechanisms, IBS rats were established by colorectal distention (CRD) in postnatal rats. The expression of large-conductance calcium and voltage-dependent potassium ion channels (BK channels) of the thoracolumbar spinal cord was examined in IBS and control rats. The effects of BK channel blockade on visceral hypersensitivity were evaluated. The interaction of BK channels and N-methyl-D-aspartate acid (NMDA) receptors was explored, and synaptic transmission at superficial dorsal horn (SDH) neurons of the thoracolumbar spinal cord was recorded by whole-cell patch clamp in IBS rats. Results The expression of the BK channels of the thoracolumbar spinal cord in IBS rats was significantly reduced. The blockade of BK channels could reduce the visceral hypersensitivity in IBS rats. There was an interaction between BK channels and NMDA receptors in the spinal cord. The frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in SDH neurons is significantly reduced in IBS rats. The blockade of BK channels depolarizes the inhibitory interneuron membrane and increases their excitability in IBS rats. Conclusions BK channels could interact with NMDA receptors in the thoracolumbar spinal cord of rats and regulate visceral hypersensitivity in IBS rats.
Time to blood, respiratory and urine culture positivity in the intensive care unit: Implications for de-escalation
Authors: Sellers Lindsey A , Fitton Kathryn M , Segovia Margaret F , Forehand Christy C , Dobbin Kevin K , Newsome Andrea Sikora 
Published: August 2021

Objectives: Concern for late detection of bacterial pathogens is a barrier to early de-escalation efforts. The purpose of this study was to assess blood, respiratory and urine culture results at 72 h to test the hypothesis that early negative culture results have a clinically meaningful negative predictive value. Methods: We retrospectively reviewed all patients admitted to the medical intensive care unit between March 2012 and July 2018 with blood cultures obtained. Blood, respiratory and urine culture results were assessed for time to positivity, defined as the time between culture collection and preliminary species identification. The primary outcome was the negative predictive value of negative blood culture results at 72 h. Secondary outcomes included sensitivity, specificity, positive predictive value and negative predictive value of blood, respiratory and urine culture results. Results: The analysis included 1567 blood, 514 respiratory and 1059 urine cultures. Of the blood, respiratory and urine cultures ultimately positive, 90.3%, 76.2% and 90.4% were positive at 72 h. The negative predictive value of negative 72-h blood, respiratory and urine cultures were 0.99, 0.82 and 0.97, respectively. Antibiotic de-escalation had good specificity, positive predictive value and negative predictive value for finalized negative cultures. Conclusion: Negative blood and urine culture results at 72 h had a high negative predictive value. These findings have important ramifications for antimicrobial stewardship efforts and support protocolized re-evaluation of empiric antibiotic therapy at 72 h. Caution should be used in patients with clinically suspected pneumonia, since negative respiratory culture results at 72 h were weakly predictive of finalized negative cultures.
Refill Friction Stir Spot Welding Al Alloy to Copper via Pure Metallurgical Joining Mechanism
Authors: Shen Zhikang , Ding Yuquan , Guo Wei , Hou Wentao , Liu Xiaochao , Chen Haiyan , Liu Fenjun , Li Wenya , Gerlich Adrian 
Published: August 2021

AbstractThe current investigation of refill friction stir spot welding (refill FSSW) Al alloy to copper primarily involved plunging the tool into bottom copper sheet to achieve both metallurgical and mechanical interfacial bonding. Compared to conventional FSSW and pinless FSSW, weld strength can be significantly improved by using this method. Nevertheless, tool wear is a critical issue during refill FSSW. In this study, defect-free Al/copper dissimilar welds were successfully fabricated using refill FSSW by only plunging the tool into top Al alloy sheet. Overall, two types of continuous and ultra-thin intermetallic compounds (IMCs) layers were identified at the whole Al/copper interface. Also, strong evidence of melting and resolidification was observed in the localized region. The peak temperature obtained at the center of Al/copper interface was 591 °C, and the heating rate reached up to 916 °C/s during the sleeve penetration phase. A softened weld region was produced via refill FSSW process, the hardness profile exhibited a W-shaped appearance along middle thickness of top Al alloy. The weld lap shear load was insensitive to the welding condition, whose scatter was rather small. The fracture path exclusively propagated along the IMCs layer of Cu9Al4 under the external lap shear loadings, both CuAl2 and Cu9Al4 were detected on the fractured surface on the copper side. This research indicated that acceptable weld strength can be achieved via pure metallurgical joining mechanism, which has significant potential for the industrial applications.
Neurodevelopmental profiles of preschool-age children in Flint, Michigan: a latent profile analysis
Authors: Zheng Shuting , Bishop Somer L. , Ceja Tiffany , Hanna-Attisha Mona , LeWinn Kaja 
Published: August 2021

Abstract
Objective
Children in Flint, Michigan, have experienced myriad sociodemographic adversities exacerbated by the Flint water crisis. To help inform child-focused prevention and intervention efforts, we aimed to describe patterns of neurodevelopmental outcomes among preschoolers who experienced the Flint water crisis before age 2 years.

Method
Participants were 170 preschoolers who completed a comprehensive neurodevelopmental assessment battery, including directly administered measures of cognitive and executive functioning and maternal-report of adaptive skills and behavioral problems. We used latent profile analysis to derive subgroups. Multivariate multinomial logistic regression was conducted to examine the predictors of profile memberships, including child sex and maternal/family-level factors selected from an array of measured exposures using least absolute shrinkage and selection operator regression.

Results
Three latent profiles were identified: Profile 1—relative weakness in all domains (50%); Profile 2—normative functioning in all domains (34.1%); and Profile 3—relative strengths in executive function and behavior (15.9%). Profile 1 showed lower scores across cognitive and behavioral domains. Profile 2 demonstrated abilities within the normal range across domains. Profile 3 showed relative strength in executive functioning with few behavior problems, despite lower cognitive performance. Children across all profiles showed adaptive behavior in the adequate range. Child sex and maternal IQ were significant predictors of profile membership.

Conclusions
Children in Flint demonstrated diverse patterns of development in the face of sociodemographic and environmental adversities. Comprehensive screening and neurodevelopmental profiling of children in this at-risk population are needed to identify areas of needs and inform appropriate service delivery.
LLAMA: a robust and scalable machine learning pipeline for analysis of large scale 4D microscopy data: analysis of cell ruffles and filopodia
Authors: Lefevre James G. , Koh Yvette W. H. , Wall Adam A. , Condon Nicholas D. , Stow Jennifer L. , Hamilton Nicholas A. 
Published: August 2021

Abstract
Background
With recent advances in microscopy, recordings of cell behaviour can result in terabyte-size datasets. The lattice light sheet microscope (LLSM) images cells at high speed and high 3D resolution, accumulating data at 100 frames/second over hours, presenting a major challenge for interrogating these datasets. The surfaces of vertebrate cells can rapidly deform to create projections that interact with the microenvironment. Such surface projections include spike-like filopodia and wave-like ruffles on the surface of macrophages as they engage in immune surveillance. LLSM imaging has provided new insights into the complex surface behaviours of immune cells, including revealing new types of ruffles. However, full use of these data requires systematic and quantitative analysis of thousands of projections over hundreds of time steps, and an effective system for analysis of individual structures at this scale requires efficient and robust methods with minimal user intervention.

Results
We present LLAMA, a platform to enable systematic analysis of terabyte-scale 4D microscopy datasets. We use a machine learning method for semantic segmentation, followed by a robust and configurable object separation and tracking algorithm, generating detailed object level statistics. Our system is designed to run on high-performance computing to achieve high throughput, with outputs suitable for visualisation and statistical analysis. Advanced visualisation is a key element of LLAMA: we provide a specialised tool which supports interactive quality control, optimisation, and output visualisation processes to complement the processing pipeline. LLAMA is demonstrated in an analysis of macrophage surface projections, in which it is used to i) discriminate ruffles induced by lipopolysaccharide (LPS) and macrophage colony stimulating factor (CSF-1) and ii) determine the autonomy of ruffle morphologies.

Conclusions
LLAMA provides an effective open source tool for running a cell microscopy analysis pipeline based on semantic segmentation, object analysis and tracking. Detailed numerical and visual outputs enable effective statistical analysis, identifying distinct patterns of increased activity under the two interventions considered in our example analysis. Our system provides the capacity to screen large datasets for specific structural configurations. LLAMA identified distinct features of LPS and CSF-1 induced ruffles and it identified a continuity of behaviour between tent pole ruffling, wave-like ruffling and filopodia deployment.
A genome epidemiological study of mycobacterium tuberculosis in subpopulations with high and low incidence rate in Guangxi, South China
Authors: Lin Dingwen , Wang Junning , Cui Zhezhe , Ou Jing , Huang Liwen , Wang Ya 
Published: August 2021

AbstractBackgroundTuberculosis (TB) is caused by a bacterium calledMycobacterium tuberculosis(Mtb). China is the third in top 8 high TB burden countries and Guangxi is one of the high incidence areas in South China. Determine bacterial factors that affected TB incidence rate is a step toward Ending the TB epidemic.ResultsGenomes ofM. tuberculosiscultures from a relatively high and low incidence region in Guangxi have been sequenced. 347 of 358(96.9%) were identified asM. tuberculosis. All the strains belong to Lineage 2 and Lineage 4, except for one in Lineage 1. We found that the genetic structure of theM. tuberculosispopulation in each county varies enormously. Low incidence rate regions have a lower prevalence of Beijing genotypes than other regions. Four isolates which harboredmutT4-48 also hadmutT2-58 mutations. It is suggested that strains from the ancestors of modern Beijing lineage is circulating in Guangxi. Strains of modern Beijing lineage (OR=2.04) were more likely to acquire drug resistances than Lineage 4. Most of the lineage differentiation SNPs are related to cell wall biosynthetic pathways.ConclusionsThese results provided a higher resolution to better understand the history of transmission ofM. tuberculosisfrom/to South China. And the incidence rate of tuberculosis might be affected by bacterial population structure shaped by demographic history. Our findings also support the hypothesis that Modern Beijing lineage originated in South China.
Prevalence and associated characteristics of anti-SARS-CoV-2 antibodies in Mexico 5 months after pandemic arrival
Authors: Díaz-Salazar Cristina , Sánchez-García Adriana , Rodríguez-Gutiérrez René , Camacho-Ortiz Adrián , Saldívar-Rodríguez Donato , González-González José Gerardo 
Published: August 2021

Abstract
Background
Seroprevalence of anti-SARS-CoV-2 antibodies is now available in several world regions to better estimate transmission dynamics. However, to date, there is no epidemiological data regarding anti-SARS-CoV-2 prevalence in Mexico. Therefore, we aimed to determine the prevalence of anti-SARS-CoV-2 antibodies and define the clinical and demographic characteristics associated with seroprevalence.

Methods
We conducted a cross-sectional serological survey in Ciudad Guadalupe, NL, Mexico. City government employees voluntarily participated during July 2020. Demographic and clinical characteristics were collected at the time of blood sampling to analyze the associated characteristics. IgM/IgG antibodies were determined using a qualitative chemiluminescent immunoassay. Descriptive statistics were used for categorical and continuous variables. Statistical significance was tested using the Chi-squared test, Student’s t-test and the Mann–Whitney. Logistic regression models and the odds ratios (adjusted and unadjusted) were used to estimate the association of demographic and clinical characteristics.

Results
Of the 3,268 participants included, 193 (5.9%, 95% CI 5.1–6.8) tested positive for IgM/IgG against SARS-CoV-2. Sex, city of residence, and comorbidities did not show any association with having IgM/IgG antibodies. A total of 114 out of 193 (59.1%) subjects with a positive test were asymptomatic, and the odds of being positive were higher in those who reported symptoms of COVID-19 in the previous four weeks to the survey (OR 4.1, 95% CI 2.9–5.5).

Conclusions
There is a low rate of SARS-CoV-2 infection among government employees that have continuously been working during the pandemic. Six in ten infections were asymptomatic, and seroprevalence is low and still far from herd immunity. Epidemiological surveillance and preventive measures should be mandatory.
A highly effective and inexpensive standardized treatment of multidrug-resistant tuberculosis: a multicenter prospective study in China
Authors: Sun Wenwen , Wu Zheyuan , Zhou Ying , Xia Fan , Tang Qin , Wang Jie , Yang Jinghui , Yu Fangyou , Yang Hua , Xiao Heping , Fan Lin 
Published: August 2021

AbstractBackgroundTo verify the efficacy and safety of an inexpensive standardized regimen for multidrug-resistant tuberculosis (MDR-TB) with low resistance to isoniazid (INH), a multicenter prospective study was conducted in eastern China.MethodsPatients diagnosed as MDR-TB with low concentration INH resistance and rifampicin resistance, second-line/injectable agents sensitive were prospectively enrolled, given the regimen of Amikacin (Ak)–Fluoroquinolones (FQs)–Cycloserine (Cs)–Protionamide (Pto)–PasiniaZid (Pa)–Pyrazinamide (Z) for 6 months followed by 12 months of FQs–Cs–Pto–Pa–Z, and then followed up for treatment outcomes and adverse events (AEs).ResultsA total of 114 patients were enrolled into the study. The overall favorable treatment rate was 79.8% (91/114). Among 91 cases with favorable treatment, 75.4% (86/114) were cured and 4.4% (5/114) were completed treatment. Regarding to unfavorable outcomes, among 23 cases, 8.8% (10/114) had failures, 8.8% (10/114) losing follow up, 0.9% (1/114) had treatment terminated due to intolerance to drugs and 1.8% (2/114) died. Treatment favorable rate was significantly higher in newly treated MDR-TB (91.7%, 33/36) than that in retreated MDR-TB (74.4%, 58/78,p0.03). The investigators recorded 42 AEs occurrences in 30 of 114 patients (26.3%). Clinicians rated most AEs as mild or moderate (95.24%, 40/42).ConclusionsThe regimen was proved to be effective, safe and inexpensive. It is suitable for specific drug resistant population, especially for newly-treated patients, which could be expected to be developed into a short-course regimen.Clinical trials registrationChina Clinical Trial Registry ChiCTR-OPC-16009380
Cesarean delivery rates, hospital readiness and quality of clinical management in Ethiopia: national results from two cross-sectional emergency obstetric and newborn care assessments
Authors: Beyene Misrak Getnet , Zemedu Theodros Getachew , Gebregiorgis Azmach Hadush , Ruano Ana Lorena , Bailey Patricia E. 
Published: August 2021

Abstract
Background
Cesarean delivery (CD) rates have reached epidemic levels in many high and middle income countries while increasingly, low income countries are challenged both by high urban CD rates and high unmet need in rural areas. The managing authority of health care institutions often plays a role in these disparities. This paper shows changes between 2008 and 2016 in Ethiopian CD rates, readiness of hospitals to provide CD and quality of clinical care, while highlighting the role of hospital management authority.

Methods
This secondary data analysis draws from two national cross-sectional studies to assess emergency obstetric and newborn care. The sample includes 111 hospitals in 2008 and 316 hospitals in 2016, and 275 women whose CD chart was reviewed in 2008 and 568 in 2016. Descriptive statistics are used to describe our primary outcome measures: population- and institutional-based CD rates; hospital readiness to perform CD; quality of clinical management, including the relative size of Robson classification groups.

Results
The national population CD rate increased from 2008 to 2016 (< 1 to 2.7%) as did all regional rates. Rates in 2016 ranged from 24% in urban settings to less than 1% in several rural regions. The institutional rate was 54% in private for-profit hospitals in 2016, up from 46% in 2008. Hospital readiness to perform CDs increased in public and private for-profit hospitals. Only half of the women whose charts were reviewed received uterotonics after delivery of the baby, but use of prophylactic antibiotics was high. Partograph use increased from 9 to 42% in public hospitals, but was negligible or declined elsewhere. In 2016, 40% of chart reviews from public hospitals were among low-risk nulliparous women (Robson groups 1&2).

Conclusions
Between 2008 and 2016, government increased the availability of CD services, improved public hospital readiness and some aspects of clinical quality. Strategies tailored to further reduce the high unmet need for CD and what appears to be an increasing number of unnecessary cesareans are discussed. Adherence to best practices and universal coverage of water and electricity will improve the quality of hospital services while the use of the Robson classification system may serve as a useful quality improvement tool.
Regional differences in cervical cancer incidence and associated risk behaviors among Norwegian women: a population-based study
Authors: Hansen Bo T. , Campbell Suzanne , Nygård Mari 
Published: August 2021

Abstract
Background
Cervical cancer incidence is influenced by screening and risk factors in the population. The main risk factor for cervical cancer is sexually transmitted human papillomavirus (HPV), which is sexually transmitted and thus associated with sexual behavior. Smoking, parity and hormonal contraceptive use may also be associated with cervical cancer risk. We compared incidence, screening coverage and risk behaviors for cervical cancer between health regions in Norway.

Methods
We obtained data on incidence of cervical cancer among Norwegian women during 1992–2016 and data on screening coverage from the Cancer Registry of Norway. We obtained data on sexual behavior and smoking from a population-based survey of 16,575 Norwegian women who were 18–45 years old in 2005.

Results
Cervical cancer incidence was higher in the northern and southeastern region than in the middle and western region (range in incidence per 100,000 person-years during 1992–2016; north: 10.5 to 14.6; southeast: 9.3 to 12.9; mid: 6.8 to 9.5; west: 8.4 to 10.0). The incidence decreased modestly in the north (average annual percentage change (95% confidence interval) − 1.0 (− 1.2 to − 0.7)) and southeast (− 0.7 (− 1.0 to − 0.3)), but did not change significantly in the mid (− 0.3 (− 1.0 to 0.4)) and west (− 0.3 (− 0.6 to 0.0)). Compared to the national average, women in the north had earlier sexual debut, more partners and higher prevalence of ever having had a sexually transmitted infection (STI), while the opposite was observed among women in the west. Women in the middle and southeastern regions tended to be similar to the national average for sexual behaviors. Although less pronounced, the prevalence of smoking showed regional patterns similar to that observed for sexual behaviors, while ever-use of hormonal contraceptives and cervical screening coverage was similar between regions.

Conclusions
There were regional differences in cervical cancer incidence during the era of nationally organized cervical screening in Norway. To some extent, these differences corresponded to regional differences in risk behavior for cervical cancer in the Norwegian female population.