bluuebunny/crossref_metadata_2025_split huggingface dataset:
High PRMT6 expression Associated With Prognosis and Immune Infiltration in Glioma
Authors: Yang Yi , Wang Zhenshuang , Long Shengrong , Huang Jinhai , Xu Chengran , Yao Mingze , Li Lun , Li Guangyu 
Published: February 2021

Abstract
Background

Glioma is characterised by easy invasion of surrounding tissues, high mortality and poor prognosis. Moreover, the prognosis of glioma is getting worse and worse with the increase of grade, which is not optimistic. Therefore, biological markers for glioma are needed in clinical to detect and evaluate the situation and prognosis of patients with glioma. In many studies, we have found that the protein arginine methyltransferase 6 (PRMT6) expression is elevated in various tumors, which is associated with prognosis of patient. However, there has been no report or study on the role of PRMT6 in glioma.
Methods

In this study, we used various tumor-related databases to analyze the mechanism of PRMT6 in tumors, especially gliomas, from bioinformatics, and carried out relevant experimental verification with tumor tissues extracted from patients during surgery. Besides, we analyzed the relationship between PRMT6 expression and immune infiltration and immune-related cells, and discussed the possible mechanisms. We also discussed the role of PRMT6 expression in glioma from mutation, clinical indicators, enrichment analysis, and immunohistochemical results.
Results

PRMT6 is significantly differentially expressed in multiple tumors, which is associated with survival and prognosis. Especially in gliomas, the PRMT6 expression gradually increased with the grade increasing. Besides, PRMT6 can be used as an independent prognostic risk factor in the nomogram and has been verified in various databases.
Conclusions

Our results indicate that high PRMT6 expression is a potential biomarker for predicting prognosis and progression of glioma.
Koos Grade IV Vestibular Schwannomas: Considerations on a consecutive series of 60 cases. Searching for the Balance Between&nbsp;Preservation of Function and Maximal Tumor Removal
Authors: Mastronardi Luciano , Campione Alberto , Boccacci Fabio , Scavo Carlo Giacobbo , Carpineta Ettore , Cacciotti Guglielmo , Roperto Raffaelino , Sufianov Albert , Zomorodi Ali 
Published: February 2021

Abstract
Purpose Koos Grade IV vestibular schwannomas (VS) (maximum diameter >3cm) compress the brainstem and displace the fourth ventricle. Microsurgical resection with attention to the right balance between preservation of function and maximal tumour removal is the treatment of choice.Methods Our series consists of 60 consecutive patients with unilateral VS, operated on from December 2010 to July 2019. All patients underwent microsurgical removal via the retrosigmoid approach. The adherence of VS’ capsule to surrounding nervous structures and the excessive tendency of tumor to bleed during debulking, because of a redundant vascular architecture, was evaluated by reviewing video records. Microsurgical removal of tumor was classified as total (T), near-total (NT: residue<5%), subtotal (ST: residue 5-10%) or partial (P: residue>10%).Results Maximal mean tumor diameter was 3,97cm (SD±1,13; range 3,1-5,8cm). Preoperative severely impaired hearing or deafness (AAO-HNS Classes C-D) was present in 52 cases (86,7%). Total or NT resection was accomplished in 46 cases (76,7%), 65,8% in cases with and 95,4% without tight adhesion of capsule to nervous structures (p<0,001). Endoscopic-assisted microsurgical removal of VS in the IAC was performed in 23 patients: in these cases a T resection was obtained in 78,3% versus 45,9% of microsurgery only (p<0,001).The capsule of VS was tightly adherent to nervous structures in 63,3% of patients, whereas hypervascular high-bleeding tumors represented 56,7%. Hearing preservation was possible in 2 out of 8 patients with preoperative class B hearing. At last follow-up, 34 patients (56,7%) had a normal postoperative FN outcome (HBI), 9 (15,0%) were HBII, 8 (13,3%) HBIII, and 9 (15,0%) HBIV. Total-NT resection of solid and low-bleeding VS, without tight capsule adhesion was associated with better FN outcome. Mortality was zero; permanent complications were observed in 2 cases (diplopia, hydrocephalus), transient in 9.Conclusions Microsurgery of Koos Grade IV VS seems to be associated with more than acceptable functional results, with high rate of T and NT removal of tumor. Long-term FN results seem to be worse in patients with cystic Koos Grade IV VS, in cases with tight capsule adherences to nervous structures and in high-bleeding tumors.
Enhanced Cancer Starvation Therapy via Autophagy Inhibition With a Functional Dendritic Mesoporous Organosilicon Nanoagent
Authors: Wu fan , Liu yang , Cheng hui , Meng yun , Yi yan , Xiao jinrong , Liu yanyan , Wu yelin 
Published: February 2021

Abstract
Glucose oxidase (GOx) can effectively catalyze glucose intogluconic acid and hydrogen peroxide (H2O2) in the presence of O2, which is considered as an attractive starvation strategy for cancer therapy. However, the autophagy phenomenon protects tumor cells from starvation therapy, limiting the therapy effect, thus autophagy inhibition could be used as a troubleshooting method to enhance tumor starvation therapy. Herein, biodegradable dendritic mesoporous organosilicon nanoagent (DMON) was used as the nanocarrier to deliver GOx and 3-MA (an autophagyinhibition agent), designed as DMON@GOx/3-MA. T his formulation could have a synergetic effect on autophagy inhibition and starvation therapy. All in vitro and in vivo results demonstrated that autophagy inhibition obviously enhanced the efficacy of starvation therapy, leading to tumor growth suppression. Our strategy will provide a new way to enhance the efficacy of starvation cancer therapy.
Natural Course of Myopic Traction Maculopathy and Factors Influencing Progression and Visual Acuity
Authors: Li Shiwei , Li Tingting , Wang Xiangning , Cai Xuan , Lu Bin , Chen Yan , Liu Chang , Wu Qiang 
Published: February 2021

Abstract
Background: To describe the natural course of myopic traction maculopathy (MTM) and determine predictive factors for its progression and visual prognosis.Methods: This retrospective observational study included 113 MTM patients (147 eyes). Best-corrected visual acuity (BCVA) measurements and optical coherence tomography findings were recorded.Results: Over a mean follow-up of 38.2 ± 11.1 months, 58 of 147 eyes (39.5%) progressed. The progression rate of outer schisis prominently located in the fovea or posterior staphyloma was significantly higher than that of outer schisis prominently located in paravascular areas (P = 0.0006). MTM with partial posterior vitreous detachment during the follow-up progressed more rapidly than MTM without (P = 0.0461). Patients with older age (>65 years), and defects in the ellipsoid zone (EZ) had worse BCVA at the last visit (P = 0.0281, and P = 0.0433). Multiple linear regression analysis showed that BCVA and defects in the EZ at baseline were significantly associated with the final BCVA (P < 0.0001 and P = 0.0259, respectively).Conclusion: MTM has a high possibility for progression. Outer schisis located predominantly in the fovea or posterior staphyloma or with partial posterior vitreous detachment exhibits rapid progression. The integrity of the EZ is related to visual prognosis.
Floods in Provence-Alpes-Côte&nbsp;D'azur and Lessons for French Flood Risk Governance
Authors: Kougkoulos Ioannis , Merad Myriam , Cook Simon J. , Andredakis Ioannis 
Published: February 2021

Abstract
France experiences catastrophic floods on a yearly basis, with significant societal impacts. In this paper, we employ a historical assessment of catastrophic past flood events in the Provence-Alpes-Côte d'Azur (PACA) region and perform Strengths-Weaknesses-Opportunities-Threats (SWOT)-analysis to evaluate some aspects of the French Flood Risk Governance (FRG) system and suggest improvements. Our evaluation shows that despite persistent government efforts, the impacts of flood events in the region do not appear to have lessened over time. Identical losses in the same locations (e.g. Riou de l’Argentière watershed) can be observed after repetitive catastrophic events (e.g. 2015, 2019) triggering local inhabitant protests. To avoid future disasters, we suggest that the French FRG should benefit from the following improvements: a) regular updates of the risk prevention plans and tools; b) the adoption of a Build Back Better logic instead of promoting the reconstruction of damaged elements in the same locations; c) taking into account undeclared damages into flood risk models (and not only those declared to flood insurance); d) increased communication between the actors of the different steps of each cycle (prepare, control, organise etc.); e) increased communication between three main elements of the cycle (risk prevention, emergency management and disaster recovery); f) an approach that extends the risk analysis outside the borders of the drainage basin (to be used in combination with the current basin risk models); and g) increased participation in FRG from local population. We also briefly discuss the use operational research methods for the optimisation of the French FRG.
Family Talk Versus Usual Services In Improving Child And Family Psychosocial Functioning In Families With Parental Mental Illness (PRIMERA - Promoting Research And Innovation In Mental Health Services For Families And Children): Study Protocol For A Randomised Controlled Trial
Authors: Furlong Mairead , McGilloway Sinead , Mulligan Christine , McGuinness Colm , Whelan Nuala 
Published: February 2021

Abstract
Background: Parental mental illness is common and can lead to dependent children incurring a high risk of developing mental disorders, physical illness, and impaired educational and occupational outcomes. Family Talk is one of the better known interventions designed to prevent the intergenerational transmission of mental illness. However, its evidence base is small, with few robust independent randomised controlled trials, and no associated process or cost evaluations. The PRIMERA (Promoting Research and Innovation in Mental hEalth seRvices for fAmilies and children) research programme involves a mixed methods evaluation of Family Talk which is being delivered in mental health settings in Ireland to improve child and family psychosocial functioning in families with parental mental illness.Methods: The study comprises a multi-centre, randomised controlled trial (RCT), with nested economic and process evaluations, to assess the clinical and cost effectiveness and implementation mechanisms of Family Talk compared to usual services. The study is being conducted in 15 adult and child mental health settings in Ireland. Families with a parent with mental illness, and children aged 5-18 years (n = 144 families) will be randomised to either the 7-session Family Talk programme (n = 96) or to standard care (n = 48) using a 2:1 allocation ratio. The primary outcomes are child psychosocial functioning and family functioning. Secondary outcomes are: understanding and experience of parental mental illness, parental mental health, child and parental resilience, partner wellbeing and service utilisation. Blind assessments will take place at pre-intervention, and at 6-, 12-month follow up.Discussion: Given the prevalence and burden of intergenerational mental illness, it is imperative that prevention through evidence-based interventions becomes a public health priority. The current study will provide an important contribution to the international evidence base for Family Talk whilst also helping to identify key implementation lessons in the scaling up of Family Talk, and other similar interventions, within routine mental health settings.Trial registration: ISRCTN Registry, ISRCTN13365858. Registered 5th February 2019. http://www.isrctn.com/ISRCTN13365858
Health services utilization of Chinese patients with Huntington's disease: A cross-sectional study
Authors: Ke Huiyi , Cao Xi , Song Yanyan , Cao Li 
Published: February 2021

Abstract
Background
Huntington’s disease (HD) is a hereditary disease which could have a large impact on patients’ quality of life. As the neurodegenerative disorders progress, HD patients are expected to regularly take follow-up medical visits for proper treatment. This study aimed to analyze the general situation of health services utilization of Chinese HD patients and factors associated with their adherence to follow-up medical visits.
Methods
We collected data from a questionnaire-based investigation conducted by Chinese Huntington's Disease Association. Data from 232 respondents were included to investigate whether they adhered to regular follow-up medical visits and the cause and correlates. Based on Andersen’s behavioral model, the independent variables were categorized into predisposing, enabling and need factors. The variables were analyzed by chi-square test and stepwise logistic regression analysis.
Results
31.9% of the respondents had regular follow-up medical visits over the past year. Univariate analysis showed there were significant differences with 6 factors (P < 0.05), among which, according to logistic regression, 2 enabling factors (reimbursement of health insurance, need for accompanying family members to follow-up visits) and 3 need factors (perceived stage of disease, perceived effectiveness of drugs, self-care ability) were independent influencing factors of follow-up medical behaviors of Chinese HD patients. The predisposing factors investigated here did not play a part in determining patients’ adherence to follow-up visits.
Conclusions
Poor adherence to medical visits among Chinese HD patients is derived from multiple factors, including reimbursement of health insurance, perceived stage of disease and effectiveness of drugs, need for accompanying family members and self-care ability. To promote HD patients’ health services utilization, the improvement of health insurance system, the enhancement of social support and the development of therapeutic approaches still have a way to go.
Patterns of reproductive isolation in a haplodiploid mite, Amphitetranychus viennensis: prezygotic isolation, hybrid inviability and hybrid sterility
Authors: Sato Yukie , Fujiwara Satoshi , Egas Martijn , Matsuda Tomoko , Gotoh Tetsuo 
Published: February 2021

Abstract
Background

Evolution of reproductive isolation is an important process, generating biodiversity and driving speciation. To better understand this process, it is necessary to investigate factors underlying reproductive isolation through various approaches but also in various taxa. Previous studies, mainly focusing on diploid animals, supported the prevalent view that reproductive barriers evolve gradually as a by-product of genetic changes accumulated by natural selection by showing a positive relationship between the degree of reproductive isolation and genetic distance. Haplodiploid animals are expected to generate additional insight into speciation, but few studies investigated the prevalent view in haplodiploid animals. In this study, we investigate whether the relationship also holds in a haplodiploid spider mite, Amphitetranychus viennensis (Zacher).
Results

We sampled seven populations of the mite in the Palaearctic region, measured their genetic distance (mtDNA) and carried out cross experiments with all combinations. We analyzed how lack of fertilization rate (as measure of prezygotic isolation) as well as hybrid inviability and hybrid sterility (as measures of postzygotic isolation) varies with genetic distance. We found that the degree of reproductive isolation varies among cross combinations, and that all three measures of reproductive isolation have a positive relationship with genetic distance. Based on the mtDNA marker, lack of fertilization rate, hybrid female inviability and hybrid female sterility were estimated to be nearly complete (99.0–99.9% barrier) at genetic distances of 0.475–0.657, 0.150–0.209 and 0.138–0.204, respectively.
Conclusions

The prevalent view on the evolution of reproductive barriers is supported in the haplodiploid spider mite we studied here. According to the estimated minimum genetic distance for total reproductive isolation in parent population crosses in this and previous studies, a genetic distance of 0.152–0.210 in mtDNA (COI) appears required for speciation in spider mites. Due to a lack of hybrid males, we could not address Haldane’s rule, which can be extended to haplodiploids, even though we focused on a young diverging group of spider mites. Our results highlight the importance of cytonuclear interactions for reproductive isolation in haplodiploid spider mites.
Predictive Study of Pharmacological Reversal for Residual Neuromuscular Blockade, Early and Late Postoperative Pulmonary Complications: A Prospective, Observational, Cohort Study
Authors: Aragón-Benedí Cristian , Pascual-Bellosta Ana , Ortega-Lucea Sonia , Visiedo-Sanchez Sara , Martinez-Ubieto Javier 
Published: February 2021

Abstract
Background
The primary objective was to assess the incidence of residual neuromuscular blockade and postoperative pulmonary complications according to spontaneous or pharmacological neuromuscular reversal, comparing neostigmine and sugammadex. The secondary objective was to present a prognostic model to predict the probability of having residual neuromuscular blockade depending on patient's comorbidities and intraoperative neuromuscular blocking agents management.
Methods
Single-center, prospective, observational, cohort study including patients undergoing surgical procedures with general anaesthesia divided into four groups: cisatracurium, cisatracurium with neostigmine antagonism, rocuronium, and rocuronium with sugammadex antagonism. A binomial generalized linear model was used to predict the residual neuromuscular blockade analyzing patients demographic data, comorbidities, intraoperative neuromuscular monitoring and pharmacological reversal for each patient group.
Results
A total of 714 patients were analyzed. According to our model, none of the studied comorbidities were a predisposing factor for an increase in residual neuromuscular blockade. However, the use of rocuronium, pharmacological reversal with sugammadex, and, particularly, neuromuscular monitoring during surgery markedly decreased the probability. When using rocuronium, avoidance of neuromuscular monitoring and pharmacological reversal led to an incidence of residual neuromuscular blockade of 41.27%; conversely, the use of neuromuscular monitoring and pharmacological reversal with sugammadex decreased the probability to 2.17%.
Conclusions
In our study, intraoperative neuromuscular monitoring and pharmacological reversal with sugammadex, unlike neostigmine, were the factors that most effectively reduced the risk of residual neuromuscular blockade as well as early postoperative pulmonary complications in PACU (upper airway obstruction and desaturation) and late postoperative pulmonary complications (pneumonia or atelectasis) within 30 days.
Development of a Bile Acid-Related Gene Signature for Predicting Survival in Patients with Hepatocellular Carcinoma
Authors: Wang Gang , Guan Jun , Yang Qin , Wu Fengtian , Shao Junwei , Zhou Qihui , Guo Zixuan , Ren Yanli , Zhu Haihong , Chen Zhi 
Published: February 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common diseases, threatening millions of patients annually. Increasing evidence supports that bile acid (BA) has an impact on HCC through inflammation, DNA damage or other mechanisms. Methods: With the data from The Cancer Genome Atlas portal, a total of 127 BA-associated genes were analyzed in HCC tumor and non-tumor samples, and then, using univariate and multivariate Cox regression, genes with correlations to the prognosis of HCC patients were identified. Then, a prediction model with identified genes was constructed for evaluating the risk of HCC patients for prognosis. Results: Twenty-six genes with differential expression between HCC and control tissue samples were identified, of which 19 genes had up-regulated expression and 7 genes had down-regulated expression in tumor samples. Three genes, NPC1, ABCC1 and SLC51B, were extrapolated to construct a prediction model for prognosis of HCC patients. Conclusion: The three-gene prediction model was more reliable than the pathological staging characters of the tumor for the prognosis and survival of HCC patients. Additionally, the up-regulated genes facilitating the transport of BAs are associated with poor prognosis of HCC patients and genes of de novo synthesis of BAs benefits HCC patients.
Dose Modifications of Ribociclib and Endocrine Therapy for Treatment of ER+ HER2- metastatic&nbsp;Breast Cancer&nbsp;
Authors: Kristensen Kristoffer Bøss , Thomsen Ida Marie Nedergaard , Berg Tobias , Kodahl Annette R. , Jensen Anders B. 
Published: February 2021

Abstract
Purpose: Treatment for estrogen receptor positive (ER+), human epidermal receptor 2 negative (HER2-) metastatic breast cancer (MBC) has improved with the approval of CDK 4/6 inhibitors. Clinical trials with the CDK4/6 inhibitor ribociclib, suggest that between 35% to 57.5% of the patients experience a dose reduction during treatment. Information on the possible consequences of dose reduction concerning efficacy is needed. Methods: A retrospective cohort study on patients with ER+ HER2- MBC from three Danish oncology departments. Data on tolerability and progression-free survival were collected from electronic health records. Results: 128 patients with ER+ HER2- MBC who initiated ribociclib treatment between 1st January 2018 to 31st March 2020 were included in our analysis. Of these patients, 48.4% required one or more dose reductions. Overall median PFS was 19.2 months (CI-95%: 14.3-NR). Patients with one or more dose reductions did not have decreased median PFS (19.2 months, CI-95%: 14.3-NR compared to 12.2 months, CI-95%: 7.3-NR. p=0.078). Frequency of adverse events were as previously reported, with grade III and IV neutropenia occurring in 45.3% and 7% of patients, respectively. Patients treated with fulvestrant versus an aromatase inhibitor and patients with lymph node involvement at baseline had lower odds of requiring dose reduction (ORa = 0.30, CI-95%: 0.18-0.89 & ORa = 0.41, CI-95%: 0.12-0.73, respectively). Conclusion: Our results indicate that dose reduction of ribociclib is safe and do not compromise the efficacy of the treatment. Furthermore, the study supports translation of results from the MONALEESA trials to patients treated in real-world clinical settings.