bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis and Construction of ceRNA Networks Reveal 4 mRNAs as Potential Biomarkers of Temozolomide-resistant Glioblastomas
Authors: Gu Sujie , Wang Yihao , Lei Deqiang , Zhao Hongyang 
Published: May 2021

Abstract
Background: Glioblastoma multiform (GBM) is the most malignant tumor of central nervous system. Although temozolomide (TMZ) was reported to improve the prognosis of GBM since applied to clinical practice, its chemoresistance has become a hot research focus and even been thought as the major factor of tumor recurrence. It’s urgent to illustrate the mechanism of TMZ resistance in GBM.Methods: In this study, we retrieved and analyzed expression profiles of TMZ resistant and TMZ sensitive glioma cell lines from GEO datasets, and then identified the enriched pathways of the differentially expressed genes. Moreover, genes involved in the KEGG pathways closely related to GBM from the Comparative Toxicogenomics 2020 Update Database were used for survival analysis of GBM samples from TCGA database. Finally, competing endogenous RNA networks regulating the acquired genes were constructed based on the expression profiles and public database. Results：To our knowledge, we firstly identified 4 mRNAs (LAMA1, COL5A1, ITGA2 and STK11) as potential biomarkers of TMZ resistant GBMs, then constructed their competing endogenous RNA networks. Conclusions: The results would provide theoretical basis for overcoming TMZ chemoresistance and contribute to more optimal chemotherapy of GBMs.
The Effects of an Innovative Integrated Care Intervention in Brazil on Local Health Service use by Dependent Older People
Authors: Lloyd-Sherlock Peter , Giacomin Karla , Sempé Lucas 
Published: May 2021

Abstract
Background:Since 2011, the Brazilian city of Belo Horizonte has been operating an innovative scheme to support care-dependent older people in disadvantaged communities: Programa Maior Cuidado (PMC – Older Person’s Care Programme). This paper examines two potential effects of inclusion in PMC on types of outpatient health service utilisation by dependent older people. The first is that being in PMC is associated with a higher frequency of outpatient visits for physical rehabilitation. The second is that being in PMC is associated with a higher frequency of planned versus unplanned outpatient visits.Methods:We apply a quasi-experimental design to a unique set of government data. First, we preprocess our sample through different matching techniques such as ‘coarsened exact matching’ (CEM), ‘nearest neighbour’ based on logit scores (NN), ‘optimal pair’ (OP) and ‘optimal full’ (OF) methods. Second, we estimate marginal effects of being in PMC on our outcomes of interest. We use univariate log-binomial regressions with robust standard errors to calculate the incidence ratio of PMC on rehabilitation and planned visits.Results:We find significant positive incidence rates for belonging to PMC for both outcomes of interest under all matching specifications. The CEM shows a higher incidence rate for the proportion of visits for rehabilitation by PMC patients compared to the non-PMC controls: 3.35 (95% CI 1.79 - 6.27). For planned visits, there are significant positive associations for being in PMC in the CEM (1.25 (95% CI 1.17 - 1.33)) and the other models.Conclusions:Our analysis reveals significant positive associations between older people included in PMC and a matched set of controls for making outpatient visits that were planned, rather than unplanned. We find similar associations for the proportion of visits made for rehabilitation, as opposed to another reason. These findings indicate that PMC influences some elements of outpatient health service utilization by dependent older people.
Streptococcus Vaginalis Sp. Nov., a Novel Bacterial Species Isolated From Vaginal Swabs of a Pregnant Woman With Diabetes
Authors: Chao An-Shine , Lin Chiao-Yun , Chao Angel , Lee Yun-Shien , Chang Yu-Chung , Wu Hui-Chung , Dai Yu-Jie , Liao Wei-Qi , Kao Fan-Ching , Chen Yi-sheng 
Published: May 2021

Abstract
Sequences targeted at the V3 and V4 16S rRNA hypervariable regions of a streptococcal strain (P1L01T) isolated from vaginal swabs of a pregnant woman with diabetes were 100% similar to those of Streptococcus anginosus subsp. whileyi. However, phylogenetic analysis based on 16S rRNA full-gene sequencing (1562 bp) revealed highest sequence similarity to Streptococcus periodonticum (98.65%), followed by Streptococcus anginosus subsp. whileyi (98.65 %), and Streptococcus anginosus subsp. anginosus (98.44%). Phylogenies of housekeeping genes rpoB and groEL were compared to improve classification, and the results showed a clear separation between strain P1L01T and closely related Streptococcus type strains. The complete genome of strain P1L01T consisted of 2,108,769 bp with a G+C content of 38.5 mol%. Average nucleotide identity values, based on genome sequencing, between strain P1L01T and Streptococcus periodonticum KCOM 2412T, Streptococcus anginosus subsp. whileyi CCUG 39159T, and Streptococcus anginosus subsp. anginosus NCTC 10713T were 95.48%, 94.33%, and 95.28%, respectively. The highest in silico DNA-DNA hybridization value with respect to the closest species was 66.2%, i.e., below the species cut-off of 70% hybridization. The main cellular fatty acids of strain P1L01T were 16:0, 18:1ω7c, and 14:0. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify this isolate as representative of a novel species of the genus Streptococcus, Streptococcus vaginalis sp. nov., in reference to its isolation from vaginal swabs, with strain P1L01T (=NBRC 114754T = BCRC 81289T) as the type strain.
The Optimization Solution of the Vertical Rolling Force by Using Unified Yield Criterion
Authors: Zhang Yufeng , Zhao Meiying , Xu Li , Di Hong Shuang , Zhou Xiaojuan , Wen Peng , Zhao Dewen , Zhang Dianhua 
Published: May 2021

Abstract
Vertical rolling is an important technique to control the width of continuous casting slab in hot rolling field. Accurate prediction of vertical rolling force is a core point to maintain rolling mill equipment. Due to the limitation of the algorithm, the prediction accuracy of most vertical rolling force models based on the energy method can only reach more than 10%. Therefore, it is challenging to optimize the rolling force model to improve the prediction accuracy. This paper presents an innovative approach for optimizing the calculation of vertical rolling force with a unified yield criterion. Firstly, the maximal width of dog bone region is determined by the slip-line method and described the dog bone shape via sine function model. Furthermore, proposed velocity and corresponding strain rate fields satisfy kinematically admissible condition is used to calculate the total power. Finally, the analytical solution of the rolling force and dog bone shape model is obtained by repeatedly optimizing the weighted coefficient b of intermediate principal shear stress on the yield criterion. And the effectiveness of the proposed mechanical model is verified by measured data in strip hot rolling field and other models’ results. The results shows that the prediction accuracy of vertical rolling force model can be improved by optimizing the value of b. Then, the impacts of reduction rate, initial thickness and friction factor on dog-bone shape size and vertical rolling force are discussed.
Structural and Gasification Kinetic Studies on Co-pyrolysis Chars of Coal and Biomass
Authors: Liu Lang , Zeng Jingsong , Jiao Qingrui , Ren Shan , Yang Jian , Su Xiangdong , Cao Yan , Tang Anjiang 
Published: May 2021

Abstract
In the present study, the CO2 gasification behaviours of the co-pyrolysis chars with coal and biomass as feedstock were investigated using isothermal thermogravimetric analysis (TGA) at 950–1150 °C. The TGA results demonstrated that the char gasification reactivity was improved at higher biomass composition. In addition, the char characteristics results evaluation revealed that biomass promoted the development of a porous structure and inhibited the formation of graphite-like carbon during the co-pyrolysis of coal and biomass. Moreover, an extended random pore model (eRPM) was proposed to define the char gasification kinetics of coal and biomass. It was found that the activation energies of chars decreased with increasing biomass composition. Lastly, the relationship between the gasification reactivity and char characteristics was evaluated.
Altered Brain Network Degree Centrality in Major Depressive Disorder via Electro-Acupuncture Stimulation at Baihui(gv20)
Authors: Li Shasha , Chen Ya , Duan Gaoxiong , Pang Yong , Liu Huimei , Wei Yichen , Liang Lingyan , Liu Peng , Deng Demao 
Published: May 2021

Abstract
Background: Although the acupuncture treatment of major depressive disorder(MDD) has been recognized by the latest clinical practice guidelines of the American Academy of Internal Medicine, complex therapeutic mechanisms need further to clarify. The aim of the study is investigate whether the aberrant resting state brain network in MDD patients could be regulated by acupuncture at GV20 using functional magnetic resonance imaging(fMRI) combined with degree centrality(DC) method. Results: Compared to healthy subjects, MDD patients exhibited significantly aberrant DC in widely brain regions, including cortical(PFC, precuneus, temporal, insula) and sub-cortical (thalamus, putamen and caudate) structures. Furthermore, results showed that acupuncture at GV20 induced down-regulation the DC of abnormal brain regions in MDD patients. Conclusions: Our findings provide imaging evidence to support that GV20-related acupuncture stimulation may modulate the abnormal brain function state in MDD patients by using fMRI technique combined with DC analysis. This study may partly interpret the neural mechanisms of acupuncture at GV20 which is used to treat patients with MDD in clinical. Trial registration: ChiCTR, ChiCTR-IOR-15006357. Registered 05 May 2015, http://www.chictr.org.cn/showproj.aspx?proj=10922.
Hibernation slows epigenetic aging in yellow-bellied marmots
Authors: Pinho Gabriela , Martin Julien , Farrell Colin , Haghani Amin , Zoller Joseph , Zhang Joshua , Snir Sagi , Pellegrini Matteo , Wayne Robert , Blumstein Daniel , Horvath Steve 
Published: May 2021

Abstract
Species that hibernate live longer than would be expected based solely on their body size. Hibernation is characterized by long periods of metabolic suppression (torpor) interspersed by short periods of increased metabolism (arousal). The torpor-arousal cycles occur multiple times during hibernation, and it has been suggested that processes controlling the transition between torpor and arousal states cause aging suppression. Metabolic rate is also a known correlate of longevity, we thus proposed the ‘hibernation-aging hypothesis’ whereby aging is suspended during hibernation. We tested this hypothesis in a well-studied population of yellow-bellied marmots (Marmota flaviventer), which spend 7-8 months per year hibernating. We used two approaches to estimate epigenetic age: the epigenetic clock and the epigenetic pacemaker. Variation in epigenetic age of 149 samples collected throughout the life of 73 females was modeled using generalized additive mixed models (GAMM), where season (cyclic cubic spline) and chronological age (cubic spline) were fixed effects. As expected, the GAMM using epigenetic ages calculated from the epigenetic pacemaker was better able to detect nonlinear patterns in epigenetic age change over time. We observed a logarithmic curve of epigenetic age with time, where the epigenetic age increased at a higher rate until females reached sexual maturity (2-years old). With respect to circannual patterns, the epigenetic age increased during the summer and essentially stalled during the winter. Our enrichment analysis of age-related CpG sites revealed pathways related to development and cell differentiation, while the season-related CpGs enriched pathways related to central carbon metabolism, immune system, and circadian clock. Taken together, our results are consistent with the hibernation-aging hypothesis and may explain the enhanced longevity in hibernators.
Effects of Supplementing Rumen-Protected Methionine on Production Performance, Apparent Digestibility, Blood Parameters, Ruminal Metabolites and Economic Effectiveness in Lactating Holstein Dairy Cows
Authors: Wei Jialin , Dou Mengying , Liu Shuai , Yan Bichuan , Li Cuiyu , Zhang Yinghui , Xiao Jianxin , Li Yuanxiao 
Published: May 2021

Abstract
Background: Because of disadvantages of excessive dietary crude protein (CP), decreasing dietary CP of dairy cows has attracted the worldwide attention. Rumen protected methionine (RPM) supplementation can allow lower CP diets and is beneficial to milk production performance, N efficiency of cows and environment. The aim of this study was to evaluate the effects of reducing dietary CP and supplementing RPM on production, digestibility of nutrients, blood parameters, ruminal metabolites and economic effectiveness in lactating Holstein dairy cows. Results: A total of 96 lactating cows (63 ± 25 d in milk; 34.4 ± 5.74 kg/d of milk production; mean ± SD) were randomly assigned to 1 of 2 treatments: diet containing 17.3% CP without RPM (control group; CON; n = 49); diet containing 16.4% CP with supplementing 15.0 g/d of RPM (treatment group; RPM; n = 47). No effect was observed of reducing dietary CP on milk yield and milk composition. The apparent digestibility of nutrients was similar between treatments. The results related to blood showed that cows in RPM group exhibited lower concentration of blood urea nitrogen than that in CON group (P < 0.001). Moreover, there were no differences between treatments on concentrations of aspartate transaminase, alanine transaminase, alkaline phosphatase, globulin and albumin. In ruminal metabolites, microbial crude protein (MCP) of dairy cows in RPM group was higher compared with CON group (P = 0.006). Ruminal volatile fatty acid (VFA) contents were not changed by treatments except that the concentrations of butyrate and isovalerate of RPM group were higher than that of CON group at 2 h after feeding (P < 0.05). In addition, supplying the diet of 16.4% CP with RPM supplementation to cows could reduce feeding cost by 0.5 $/d per cow and boost net profits. Conclusions: Lower dietary CP with RPM supplementation did not limit milk yield, milk composition and apparent digestibility of nutrients, and could improve nitrogen utilization of dairy cows and synthesis of MCP in rumen, change VFA production at 2 h after feeding, as well as boost the economic benefits of the dairy farms.
Contrasting Monetary and non-monetary measures of poverty in developing countries: a survey
Authors: Azami Hicham El 
Published: May 2021

Abstract
While the study of poverty is popular amongst academia, there is yet to be a consensus on what defines such a phenomenon. Basing their arguments on different conceptual frameworks, some argue for a definition in monetary terms, as a lack of income or consumption, while others support a non-monetary approach focusing on shortcomings in diverse dimensions (health, education, nutrition…). Does it really matter that researchers do not agree on a definition of a poverty? Probing the empirical literature comparing monetary and non-monetary poverty in developing countries, this survey argues that different definitions of poverty lead to different populations identified as poor. Indeed, cross-tabulating poverty headcounts, it finds that a sizable portion of the non-monetary poor are excluded by the monetary approach. Moving to a subgroup level, it finds nonmonetary poverty amongst the monetary richest, and considerable disagreements with regards to poverty risks. In addition, these significant differences are not resolved when switching to a dynamic setting through panel studies. Policy implications are important: the risk mistargeting and misevaluation of policies is high if we do not consider both approaches concomitantly without one taking precedence over the other.
Idiopathic and Radiation-Induced Myxofibrosarcoma In Head And Neck-Case Report And Literature Review
Authors: Zhang Bin , Bai Miao , Tian Runfa , hao shuyu 
Published: May 2021

Abstract
BackgroundMyxofibrosarcoma (MFS) is a rare malignant fibroblastic tumor that primarily occurred in proximal extremities of older people. However, MFS, especially radiation-Induced MFS is extremely rare in the head and neck. Local recurrence is very common, the diagnosis and treatment of MFS is always a great challenge. We presented one case of radiation-induced MFS, combined with previous literature, the clinical features, essentials of diagnosis and treatment modalities of MFS in head and neck were reviewed to better understand this rare entity.Case presentationHere, we reported a case of radiation-Induced MFS under left occipital scalp in a 20-year-old girl with a history of medulloblastoma surgery and radiotherapy in 2006. A total tumor resection was performed with preservation of the overlying scalp the underlying bone, and no adjuvant therapy was administered after surgery. Post-operative pathological diagnosis was high-grade MFS. Unfortunately, the tumor relapsed six month later. Then a planned extensive resection was carried out, followed by radiotherapy. In addition, intra-operative frozen section was sent to confirm negative surgical margin. No relapse occurred in 12-month postoperative follow-up.ConclusionsPlanned gross total resection (GTR) with negative margins is the reasonable choice and footstone of other treatments for MFS. Ill-defined infiltrated borders and the complicated structures make it a great trouble to achieve total resection of MFS in the head and neck. Therefore, adjuvant radiotherapy and chemotherapy seem more necessary for these lesions. Further studies are needed to confirm the efficacy of radio-chemotherapy for head and neck MFS.
Detailed imaging of mitochondrial transport and precise manipulation of mitochondrial function with genetically-encoded photosensitizers in adult Drosophila neurons
Authors: Mattedi Francesca , Chennell George , Vagnoni Alessio 
Published: May 2021

Abstract

Precise distribution of mitochondria is essential for maintaining neuronal homeostasis. Although detailed mechanisms governing the transport of mitochondria have emerged, it is still poorly understood how the regulation of transport is coordinated in space and time within the physiological context of an organism. How alteration in mitochondrial functionality may trigger changes in organellar dynamics also remains unclear in this context. Therefore, the use of genetically-encoded tools to perturb mitochondrial functionality in real time would be desirable. Here we describe methods to interfere with mitochondrial function with high spatiotemporal precision with the use of photosensitisers in vivo in the intact wing nerve of adult Drosophila. We also provide details on how to visualise the transport of mitochondria and to improve the quality of the imaging to attain super-resolution in this tissue.
Machine learning approaches to predict gestational age in normal and complicated pregnancies via urinary metabolomics analysis
Authors: Yamauchi Takafumi , Ochi Daisuke , Matsukawa Naomi , Saigusa Daisuke , Ishikuro Mami , Obara Taku , Tsunemoto Yoshiki , Kumatani Satsuki , Yamashita Riu , Tanabe Osamu , Minegishi Naoko , Koshiba Seizo , Metoki Hirohito , Kuriyama Shinichi , Yaegashi Nobuo , Yamamoto Masayuki , Nagasaki Masao , Hiyama Satoshi , Sugawara Junichi 
Published: May 2021

Abstract
The elucidation of dynamic metabolomic changes during gestation is particularly important for the development of methods to evaluate pregnancy status or achieve earlier detection of pregnancy-related complications. Some studies have constructed models to evaluate pregnancy status and predict gestational age using omics data from blood biospecimens; however, less invasive methods are desired. Here we propose a model to predict gestational age, using urinary metabolite information. In our prospective cohort study, we collected 2,741 urine samples from 187 healthy pregnant women, 23 patients with hypertensive disorders of pregnancy, and 14 patients with spontaneous preterm birth. Using gas chromatography-tandem mass spectrometry, we identified 184 urinary metabolites that showed dynamic systematic changes in healthy pregnant women according to gestational age. A model to predict gestational age during normal pregnancy progression was constructed; the correlation coefficient between actual and predicted weeks of gestation was 0.86. The predicted gestational ages of cases with hypertensive disorders of pregnancy exhibited significant progression, compared with actual gestational ages. This is the first study to predict gestational age in normal and complicated pregnancies by using urinary metabolite information. Minimally invasive urinary metabolomics might facilitate changes in the prediction of gestational age in various clinical settings.
Steinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland
Authors: Lis Magdalena , Sajnaga Ewa , Skowronek Marcin , Wiater Adrian , Rachwał Kamila , Kazimierczak Waldemar 
Published: May 2021

Abstract
A new species of entomopathogenic nematodes, Steinernema sandneri n. sp., was recovered by baiting from Poland. Its morphological traits indicate that the new species is a member of the feltiae-kraussei group. A body length of 843 (708–965) μm, a more anterior position of excretory pore (56 μm), and the lower D% value (40 vs > 46) discriminate this species from most of the other group members. The first-generation males of S. sandneri n. sp. can be distinguished from the other clade members by a 60 μm long spicule, a relatively long gubernaculum (GS% = 79), and the position of the excretory pore (80 μm). Phylogenetic analysis of the ITS rDNA, D2D3 of 28 S rDNA, and cox1 sequences confirmed that S. sandneri n. sp. is a new species of the feltiae-kraussei group, closely related to S. kraussei and S. silvaticum.
Biomass generation and biodiesel production from macroalgae grown in the irrigation canal wastewater
Authors: Whangchai Kanda , Souvannasouk Vannasinh , Bhuyar Prakash , Ramaraj Rameshprabu , Unpaprom Yuwalee 
Published: May 2021

Abstract
The wastewater concentration is commonly acceptable for macroalgae growth; this process consumes water and is applicable for bioremediation. This study evaluated biodiesel's potential production from freshwater macroalga, Nitella sp., using batch experiment. Algae were collected from wastewater saturated from irrigation canals. Water quality and algae growth environment characteristics were monitored and analyzed. COD and BOD values were 18.67 ± 4.62 mg/L and 5.40 ± 0.30 mg/L, respectively. The chemical composition contents were high, demonstrating that water quality and sufficient nutrients could support algae growth. Oil extraction was estimated by the room temperature and heat extraction methods. The biodiesel in room temperature treatment was 0.0383 ± 0.014%, and in heat, extraction treatment was 0.0723 ± 0.029%. Results confirmed that the heat extraction treatment gave a high amount of oil and biodiesel yield. Gas chromatography/mass spectrometry (GC/MS) was used to analyze fatty acid methyl esters (FAME). Results revealed that 9-octadecane was a major portion of the substance. The obtained results confirmed that the wastewater contains many elements that can be utilized for dual-mode, like bioremediation and enhanced macroalgae growth for biodiesel production. Therefore, macroalgae grown in canal wastewater were highly feasible for use in sustainable biodiesel production.