bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feline adipose-derived mesenchymal stem cells induce effector phenotype and enhance cytolytic function of CD8+ T cells
Authors: Taechangam Nopmanee , Walker Naomi J. , Borjesson Dori L. 
Published: September 2021

Abstract
Background
Feline adipose-derived mesenchymal stem cells (ASCs) engage with a variety of immune cells and have been used in several clinical trials for the treatment of inflammatory and immune-dysregulated diseases in cats, but the impact they exert on the functional characteristics on T cells, particularly CD8+ T cells, remains to be elucidated.

Methods
Modified mixed leukocyte reaction was performed between feline ASCs and PBMCs. Changes of cell cycle stages, phenotype and cellular senescence were determined through flow cytometry and gene expression analysis. Cytotoxicity assay was performed to evaluate CD8+ T cell effector function.

Results
Feline ASCs induce cell cycle arrest on CD8+ T cells in a contact-dependent manner, downregulate CD8 surface expression, and shift their phenotype toward terminally differentiated effector cells (CD57+, CD45R+, CD62L−). CD8 T cells interacted with feline ASCs also upregulated granzyme B, IL-2 and KLRG-1 expression and have enhanced cytotoxic potential, evident by the increased percentage of lysis on target cells.

Conclusions
Our findings suggest that feline ASCs (1) alter CD8+ T cells toward terminally differentiated, proinflammatory effector phenotype with limited proliferative capacity, and (2) enhance their cytotoxic potential through granzyme B upregulation. These cytotoxic CD8+ T cells could aid in disease cure in cases caused by an underlying, unresolved viral infection.
Quantitative SPECT/CT parameters of myocardial 99mTechnetium-3,3-diphosphono-1,2-propanodicarboxylic acid (DPD) uptake in suspected cardiac transthyretin amyloidosis
Authors: Ben-Haim Simona , Chicheportiche A. , Goshen E. , Arad M. , Smekhov M. , Menezes L. J. , Elliott P. M. , O’Mahoney E. , Stern E. , Yuzefovich Bella , Bomanji J. B. 
Published: September 2021

Abstract
Background
99mTc-labelled bisphosphonates are used for imaging assessment of patients with transthyretin cardiac amyloidosis (ATTR). Present study evaluates whether quantitative SPECT/CT measurement of absolute myocardial 99mTc-labelled 3,3-diphosphono-1,2-propanodicarboxylic acid (Tc-DPD) uptake can diagnose patients with suspected ATTR.

Methods
Twenty-eight patients (25 male, age 80.03 ± 6.99 years) with suspected ATTR referred for Tc-DPD imaging had planar and SPECT/CT imaging of the chest. Three operators independently obtained Tc-DPD myocardial SUVmax and SUVmean above threshold (SMaT) (20, 40 and 60% of SUVmax), using a semi-automated threshold segmentation method. Results were compared to visual grading (0–3) of cardiac uptake.

Results
Twenty-two patients (78%) had cardiac uptake (2 grade 1, 15 grade 2, 5 grade 3). SUVmax and SMaT segmentation thresholds enabled separating grades 2/3 from 0/1 with excellent inter- and intra-reader correlation. Cut-off values 6.0, 2.5, 3 and 4 for SUVmax, SMaT20,40,60, respectively, separated between grades 2/3 and 0 /1 with PPV and NPV of 100%. SMaT20,40,60(cardiac)/SUVmean (liver) and SMaT20,40,60(cardiac)/SUVmean(liver/lung) separated grades 2 and 3.

Conclusion
Quantitative SPECT/CT parameters of cardiac Tc-DPD uptake are robust, enabling separation of patients with grades 2 and 3 cardiac uptake from grades 0 and 1. Larger patient cohorts will determine the incremental value of SPECT/CT quantification for ATTR management.
Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites
Authors: Benedek Tibor , Szentgyörgyi Flóra , Gergócs Veronika , Menashe Ofir , Gonzalez Perla Abigail Figueroa , Probst Alexander J. , Kriszt Balázs , Táncsics András 
Published: September 2021

AbstractHere, we report and discuss the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites. Strain BFB1_13 was capable of degrading all the six BTEX-compounds under both aerobic (O2 conc. 8 mg l−1) and micro-aerobic/oxygen-limited (O2 conc. 0.5 mg l−1) conditions using either individual (8 mg‧l−1) or a mixture of compounds (~ 1.3 mg‧l−1 of each BTEX compound). The BTEX biodegradation capability of SBP-encapsulated cultures (SBP—Small Bioreactor Platform) was also assessed. The fastest degradation rate was observed in the case of aerobic benzene biodegradation (8 mg l−1 per 90 h). Complete biodegradation of other BTEX occurred after at least 168 h of incubation, irrespective of the oxygenation and encapsulation. No statistically significant difference was observed between aerobic and microaerobic BTEX biodegradation. Genes involved in BTEX biodegradation were annotated and degradation pathways were predicted based on whole-genome shotgun sequencing and metabolic analysis. We conclude that V. paradoxus strain BFB1_13 could be used for the development of reactive biobarriers for the containment and in situ decontamination of BTEX contaminated groundwater plumes. Our results suggest that V. paradoxus strain BFB1_13—alone or in co-culture with other BTEX degrading bacterial isolates—can be a new and efficient commercial bioremediation agent for BTEX contaminated sites.
Understanding the mechanisms generating outcomes in a Danish peer support intervention for socially vulnerable people with type 2-diabetes: a realist evaluation
Authors: Garn Stine Dandanell , Glümer Charlotte , Villadsen Sarah Fredsted , Malling Gritt Marie Hviid , Christensen Ulla 
Published: September 2021

Abstract
Background
Despite an increasing use and positive effects of peer support interventions, little is known about how the outcomes are produced. Thus, it is essential not only to measure outcomes, but also to identify the mechanisms by which they are generated. Using a realist evaluation approach, we aimed to identify the mechanisms generating outcomes in a Danish peer support intervention for socially vulnerable people with type 2-diabetes (peers). By investigating the participating peers’ interactions, we furthermore examined how their individual contextual factors either facilitated or hindered the mechanisms in operation.

Methods
We used a multi-method case-study design (n = 9). Data included semi-structured interviews with four key groups of informants (peer, peer supporter, project manager, and a diabetes nurse) for each case (n = 25). Furthermore, we collected survey data from peers both before and after participation (n = 9). The interview data were analysed using a systematic text condensation, and the Intervention-context-actor-mechanism-outcome framework was used to structure the analysis.

Results
We identified 2 groups of mechanisms that improved diabetes self-management and the use of healthcare services (outcomes): ‘perceived needs and readiness’ and ‘encouragement and energy’. However, the mechanisms only generated the intended outcomes among peers with a stable occupation and financial situation, a relatively good health condition, and sufficient energy (all defined as contextual factors). Independent of these contextual factors, ‘experience of social and emotional support’ was identified as a mechanism within all peers that increased self-care awareness (defined as output). Dependent on whether the contextual factors facilitated or hindered the mechanisms to generate outcomes, we categorised the peers into those who achieved outcomes and those who did not.

Conclusions
We identified two groups of mechanisms that improved the peers’ diabetes self-management and use of healthcare services. The mechanisms only generated the intended outcomes if peers’ individual contextual factors facilitated an active interaction with the elements of the intervention. However, independent of these contextual factors, a third group of mechanisms increased self-care awareness among all peers. We highlight the importance of contextual awareness of the target groups in the design and evaluation of peer support interventions for socially vulnerable people with type 2-diabetes.

Trial registration
ClinicalTrials.gov, Retrospective Registration (20 Jan 2021), registration number NCT04722289.
Effects of different planes of milk feeding and milk total solids concentration on growth, ruminal fermentation, health, and behavior of late weaned dairy calves during summer
Authors: Shiasi Sardoabi R. , Alikhani M. , Hashemzadeh F. , Khorvash M. , Mirzaei M. , Drackley J. K. 
Published: September 2021

Abstract
Background
In recent years, there has been an increasing interest in using high quantities of milk or milk replacer (MR) in heat-stressed calves to alleviate the negative effects of high environmental temperatures on their performance. However, observations have indicated a decline in growth performance in the weaning and post-weaning period, which might be optimized with increasing total solids (TS) in milk and weaning age. This study aims to optimize the effects of higher quantities of milk on late weaned calves' performance by increasing TS concentration or delivery route in summer conditions.

Method
Forty-eight newborn Holstein calves were used in a 2 × 2 factorial arrangement with the factors of pre-weaning total plane of milk (PM) intake (low vs. high) and milk TS content (12% vs. 17%). The treatments were (1) low PM (LPM) intake with 12% TS (TS intake = 45.9 kg), (2) LPM intake with 17% TS (TS intake = 65.1 kg), (3) high PM (HPM) intake with 12% TS (TS intake = 63.7 kg); and (4) HPM intake with 17% TS (TS intake = 90.3 kg). Calves were weaned at d 83, and the study was terminated at d 103 of age. Performance data (every 10 day), skeletal growth (d 80 and 100), ruminal fermentation parameters (d 48 and 91), and behavioral measurements (d 69, 70, 93 and 94) were analyzed as repeated measurements with PROC MIXED of SAS 9.4 (SAS Institute Inc., Cary, NC).

Results
Calves receiving HPM consumed less PMR from d 44 to 83 of age, but they had higher ADG from d 24 to 53 of age compared to those fed LPM (PM × age, P < 0.001). In addition, calves receiving milk with 17% TS had lower PMR intake from d 14 to 83 of age, but greater ADG from d 34 to 53 compared to those receiving milk with 12% TS (TS × age, P < 0.001). Calves that received HPM had greater skeletal growth parameters compared to LPM-fed calves, with a similar effect evident for calves fed milk with 17% TS compared with those fed milk with 12% TS. Calves receiving milk with 17% TS had greater fecal scores and diarrhea occurrence than those fed milk with 12% TS in HPM, but not LPM.

Conclusions
Increasing PM and milk TS concentration improved growth in summer-exposed calves as demonstrated by increased pre-weaning ADG, pre- and post-weaning BW, and structural growth.
Bee sting presumed endophthalmitis: a devastating ocular outcome
Authors: Al Amry Mohammed , Al Ghadeer Huda , Al Gethami Ahmed R. 
Published: September 2021

AbstractBackgroundTo report a rare case of bee sting presumed endophthalmitis that resulted in a devastating ocular outcome.Case presentationA 43-year-old patient presented 24 h after bee sting ocular injury in his left eye. He had a mild inflammatory sign at presentation, and he underwent surgical exploration to rule out a scleral defect, which revealed a sealed defect. During his hospital course, he developed signs of endophthalmitis 48 h following trauma for which he received vitreous tap and intravitreal antibiotic. Microbial culture revealed gram-negative rods,Pseudomonas aeruginosa, andAeromonas veronii. Condition escalated to reach the panophthalmitis stage and cellulitis like picture with visual acuity of no light perception. Visual evoked response (VER) demonstrated a flat response. Infection was controlled by evisceration of the globe at the end as salvage therapy against the spreading of infectionConclusionsBee sting ocular injury is an exceedingly rare type of ocular trauma. Concomitant infection can happen, and severity depends on the pathogen involved. It is crucial to have insight and start appropriate treatment based on to the patient presentation.
Use of the PROMIS-10 global health in patients with chronic low back pain in outpatient physical therapy: a retrospective cohort study
Authors: Pak Sang S. , Miller Matthew J. , Cheuy Victor A. 
Published: September 2021

Abstract
Background
Although evidence-based guidelines for physical therapy for patients with chronic low back pain (cLBP) are available, selecting patient-reported outcome measures to capture complexity of health status and quality of life remains a challenge. PROMIS-10 Global Health (GH) may be used to screen for impactful health risks and enable patient-centered care. The purpose of this study was to investigate the interrelationships between PROMIS-10 GH scores and patient demographics, health status, and healthcare utilization in patients with cLBP who received physical therapy.

Methods
A retrospective review of de-identified electronic health records of patients with cLBP was performed. Data were collected for 328 patients seen from 2017 to 2020 in three physical therapy clinics. Patients were grouped into HIGH and LOW initial assessment scores on the PROMIS-10 Global Physical Health (PH) and Global Mental Health (MH) measures. Outcomes of interest were patient demographics, health status, and healthcare utilization. Mann–Whitney U and chi-square tests were used to determine differences between groups, and binary logistic regression was used to calculate odds ratios (OR) to determine predictors of PH-LOW and MH-LOW group assignments.

Results
The PH-LOW and MH-LOW groups contained larger proportions of patients who were African American, non-Hispanic, and non-commercially insured compared to PH-HIGH and MH-HIGH groups (p < .05). The PH-LOW and MH-LOW groups also had a higher Charlson comorbidity index (CCI), higher rates of diabetes and depression, and more appointment cancellations or no-shows (p < .05). African American race (OR 2.54), other race (2.01), having Medi-Cal insurance (OR 3.37), and higher CCI scores (OR 1.55) increased the likelihood of being in the PH-LOW group. African American race (OR 3.54), having Medi-Cal insurance (OR 2.19), depression (OR 3.15), kidney disease (OR 2.66), and chronic obstructive pulmonary disease (OR 1.92) all increased the likeihood of being in the MH-LOW group.

Conclusions
Our study identified groups of patients with cLBP who are more likely to have lower PH and MH scores. PROMIS-10 GH provides an opportunity to capture and identify quality of life and global health risks in patients with cLBP. Using PROMIS-10 in physical therapy practice could help identify psychosocial factors and quality of life in the population with cLBP.
Status of CAS global ionospheric maps after the maximum of solar cycle 24
Authors: Li Zishen , Wang Ningbo , Liu Ang , Yuan Yunbin , Wang Liang , Hernández-Pajares Manuel , Krankowski Andrzej , Yuan Hong 
Published: September 2021

AbstractAs a new Ionosphere Associate Analysis Center (IAAC) of the International GNSS Service (IGS), Chinese Academy of Sciences (CAS) started the routine computation of the real-time, rapid, and final Global Ionospheric Maps (GIMs) in 2015. The method for the generation of CAS rapid and final GIMs and recent updates are presented in the paper. The quality of CAS post-processed GIMs is assessed during 2015–2018 after the maximum of solar cycle 24. To perform an independent and fair assessment, Jason-2/3 Vertical Total Electron Contents (VTEC) are first used as the references over the ocean. GPS differential Slant TECs (dSTEC) generated from 55 Multi-GNSS Experimental (MGEX) stations of the IGS are also employed, which provides a complementing way to evaluate the ability of electron content models to reproduce the spatial and temporal gradients in the ionosphere. During the test period, Jet Propulsion Laboratory (JPL) GIMs present significantly positive deviations compared to the Jason VTEC and GPS dSTEC. Technical University of Catalonia (UPC) rapid GIM UQRG exhibits the best performance in both Jason VTEC and GPS dSTEC analysis. The CAS GIMs show comparable performance with the results of the first four IAACs of the IGS. As expected, the poor performance of all GIMs is in equatorial regions and the high latitudes of the southern hemisphere. The consideration of generating multi-layer or three-dimensional ionospheric maps is emphasized to mitigate the inadequacy of ionospheric single-layer assumption in the presence of pronounced latitudinal gradients. The use of ionospheric observations from the new GNSS constellations and other space- or ground-based observation techniques is also suggested in the generation of future GIMs, given the sparse GPS/GLONASS stations in the southern hemisphere.
Silver nanoparticles and Chlorella treatments induced glucosinolates and kaempferol key biosynthetic genes in Eruca sativa
Authors: Sayed Ahmed Hanan I. , Elsherif Doaa E. , El-Shanshory Adel R. , Haider Ashraf S. , Gaafar Reda M. 
Published: September 2021

Abstract
Background
Microalgae and nanoparticles are currently considered promising tools for numerous agricultural and biotechnological applications. The green microalga Chlorella sp. MF1 and its biosynthesized silver nanoparticles (AgNPs) were used in this study as biofortification agents to enhance glucosinolate and kaempferol levels in Eruca sativa. UV–visible spectroscopy, XRD, FTIR and TEM were comprehensively used for characterizing Chlorella-based AgNPs.

Results
The biosynthesized AgNPs were found to be spherical in shape, with size ranging from 1.45 to 5.08 nm. According to FTIR measurements, silver ions were reduced to AgNPs by functional groups such as amide, hydroxyl and carboxylate. Different experimental treatments were conducted, including either soaking seeds of E. sativa or foliar spray with various concentrations of Chlorella suspension (1, 2, 3 and 4 g L−1) and AgNPs (5, 10, 20 and 40 mg L−1). Expression levels of five key genes in the biosynthetic pathway of glucosinolates (MAM1, SUR1, MYB34 and MYB51) and kaempferol (CHS) were assessed using qRT-PCR. The results indicated an upregulation in the gene expression levels in all treatments compared to control, recording the highest level at 40 mg L−1 AgNPs and 4 g L−1Chlorella suspension. In addition, high glucosinolates and kaempferol content was detected in plants whose leaves were sprayed with AgNPs and Chlorella suspension (40 mg L−1 and 4 g L−1) based on HPLC analysis. Sequence analysis of amplified CHS fragments from E. sativa plants treated with AgNPs (40 mg L−1) showed high sequence similarity to A. thaliana CHS gene. However, there were several CHS regions with sequence polymorphism (SNPs and Indels) in foliar sprayed plants.

Conclusions
Results of this study evidenced that the application of AgNPs and Chlorella suspension increased glucosinolates and kaempferol content in E. sativa through upregulation of key genes in their biosynthetic pathway.
Cultural and creative sectors at a crossroad: from a mainstream process towards an active engagement
Authors: Ost Christian , Saleh Ruba 
Published: September 2021

AbstractThe COVID-19 pandemic has led to a current global health crisis with dreadful repercussions all over the world. A global economic recession is anticipated, with strong impacts in all economic and social sectors, including the cultural sector. Although all sub sectors will be impacted (heritage sites, theatres, museums, operas, art galleries), the cultural built heritage is particularly at stake, as it relies on multiple stakeholders through a wide range of heritage-related activities (tourism, recreation, housing, real estate, construction, craftsmanship, etc.). Sites management and heritage conservation have not only been vulnerable to strong economic and social disruptions, like most of other cultural fields, but have been greatly challenged because heritage values and the paradigm of conservation (50 years after adoption of the UNESCO convention) are being themselves revisited in the perspective of the Sustainable Development Goals. The paper aims also to consider cultural heritage as part of the Cultural and Creative Sectors (CCS) and how creativity and innovation contribute to post-COVID recoveries through Schumpeter-related creative destruction process. The current crisis might be perceived in a perspective of long wave theory of innovations and economic growth. The economic history is filled with many examples of such transition period when inventions, innovations, and growth reactivate the economic development in an upward long-term trend. In such framework, crisis can trigger innovation and creativity and can be understood as opportunity to increase the CCS resilience and sustainability, as well as harness the universality and the power of creativity. Finally, the paper aims to describe implications of such situation by providing to the CCS ways to learn and experience cultural entrepreneurship, resilient strategies, new sustainable and circular business models applied to the cultural heritage sector and its conservation.
Large-for-Gestational-Age, Leptin, and Adiponectin in Infancy
Authors: Huang Rong , Dong Yu , Levy Emile , Julien Pierre , Marc Isabelle , He Hua , Xu Ya-Jie , Wei Shu-Qin , Fraser William D , Luo Zhong-Cheng 
Published: September 2021

Abstract

Context
Fetal overgrowth “programs” an elevated risk of obesity and type 2 diabetes in adulthood. Plausibly, adipokines may be involved in programming metabolic health.

Objective
This work aimed to evaluate whether large-for-gestational-age (LGA), an indicator of fetal overgrowth, is associated with altered circulating leptin and adiponectin levels in infancy, and assess the determinants.

Methods
In the Canadian 3D birth cohort, we studied 70 LGA (birth weight &gt; 90th percentile) and 140 optimal-for-gestational-age (OGA, 25th-75th percentiles) infants matched by maternal ethnicity, smoking, and gestational age at delivery. The primary outcomes were fasting leptin, and total and high-molecular-weight (HMW) adiponectin concentrations at age 2 years.

Results
LGA infants had higher body mass index (BMI) than OGA infants. However, there were no significant differences in leptin, and total and HMW adiponectin concentrations. Leptin concentrations were positively associated with female sex, weight (z score) gain 0 to 24 months, current BMI, and the sum of triceps and subscapular skinfold thickness, and negatively associated with maternal age and White ethnicity. Female sex was associated with lower total and HMW adiponectin concentrations. Weight (z score) gain 0 to 24 months and current BMI were positively correlated with total and HMW adiponectin concentrations in LGA infants only.

Conclusion
This study is the first to demonstrate that LGA does not matter for circulating leptin and adiponectin concentrations in infancy, and there may be LGA-specific positive associations between weight gain or current BMI and adiponectin concentrations in infancy, suggesting dysfunction in establishing the adiposity-adiponectin negative feedback loop in LGA individuals.