bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_Circular RNA_0001013 Exerts Oncogenice Effects in Gastric Cancer via the MicroRNA-136/TWSG1 Axis
Authors: Gao Zhaofeng , Hu Lingyu , Chen Fei , He Chunhua , Hu Biwen , Wang Xiaoguang 
Published: November 2021

Abstract
Background：Gastric cancer (GC) is one of the most principle malignant cancers in the digestive system. Moreover, the critical role of circular RNAs (circRNAs) has been identified in GC development. Methods：In this context, the purpose of research was to explore the regulatory mechanism circ_0001013, a novel circRNAs predicted by our research, in GC. The differential circRNAs and related mechanism in GC were predicted by microarray analysis. Circ_0001013, miR-136, and TWSG1 expression in GC clinical samples and cells was detected by RT-qPCR. The relationship among circ_0001013, miR-136, and TWSG was assessed by dual-luciferase reporter assay, biotin coupled probe pull-down assay, and biotin coupled miRNA capture. After gain- and loss-of-function assays in GC cells, cell proliferation, migration, invasion, and cell cycle and apoptosis were measured by EdU assay, scratch test, Transwell assay, and flow cytometry respectively. The effect of circ_0001013 on tumor growth was detected by xenograft tumor in nude mice. Results ：Microarray analysis predicted a novel circRNA, circ_0001013, was upregulated in GC, which was confirmed by RT-qPCR detection in GC tissues and cells. Besides, miR-136 was downregulated but TWSG1 was highly expressed in GC tissues. Mechanically, circ_0001013 could bind to miR-136, and miR-136 negatively targeted TWSG1 in GC cells. Silencing circ_0001013 or TWSG1 or overexpressing miR-136 decreased GC cell proliferation, migration, invasion, and cell cycle arrest and accelerated cell apoptosis. Circ_0001013 silencing decreased TWSG1 expression and inhibited transplanted tumor growth in nude mice. Conclusion：Circ_0001013 elevated TWSG1 expression by binding to miR-136, thereby exerting oncogenic effect in GC.
Radical Versus Non-Radical Resection for Small intestinal Gastrointestinal Stromal Tumors: A Propensity Score-Matched Analysis
Authors: Yin Linlin , Yan Haihao , Lin Jue , Ji Zuhong , Ji Guozhong , Zhang Xiuhua 
Published: November 2021

Abstract
Surgical resection is the first choice for the treatment of small intestinal gastrointestinal stromal tumors (GISTs), but the best surgical method for small intestinal stromal tumors remains undefined. It is not clear whether there is a difference in the long-term survival of small intestinal GISTs between radical surgery and non-radical surgery. We included 877 patients with small intestinal stromal tumors who underwent surgery between 2010 and 2015 from the SEER database. They were divided into the radical resection group and the non-radical resection group. To minimized the selection bias and mixed bias in the comparison, propensity score matching (PSM) and multivariate regression analysis were carried out. In the entire cohort, 120 patients underwent radical surgery and 757 patients received non-radical resection. The 1, 3, and 5-year OS rates were 95.7%, 80.2%, and 69.6% in the radical resection group versus 94.3%, 86.8%, and 77.2% in the non-radical resection group, respectively. (p=0.069) Meanwhile, radical resection had the similar CSS rates of 1, 3 and 5-year compared with non-radical surgery. (1-year CSS rate: 97.4% vs. 98.0%, 3-year CSS rate: 86.1% vs. 93.2%, 5-year CSS rate: 81.6% vs. 88.3%; p =0.056) Besides, after adjusting for other clinical factors and PSM, the long-term OS and CSS did not significantly differ between radical surgery and non-radical surgery. Our study preliminarily found that for small intestinal GISTs, there was no significant difference in long-term survival between radical surgery and non-radical surgery.
The survival rate of extremely low birth weight infants improved in Guangdong Province, China
Authors: Wu Fan , Jia Chun-Hong , Feng Zhou-Shan , Lin Xiao-Jun , Cui Qi-Liang , Han Sha-Sha , Jin Ya , Liu Guo-Sheng , Yang Chuan-Zhong , Ye Xiao-Tong , Dai Yi-Heng , Liang Wei-Yi , Ye Xiu-Zhen , Mo Jing , Ding Lu , Wu Ben-Qing , Chen Hong-Xiang , Li Chi-Wang , Zhang Zhe , Rong Xiao , Huang Wei-Min , Shen Wei , Yang Bing-Yan , Lv Jun-Feng , Huo Le-Ying , Huang Hui-Wen , Rao Hong-Ping , Yan Wen-Kang , Yang Yong , Ren Xue-Jun , Liu Dong , Wang Fang-Fang , Diao Shi-Guang , Liu Xiao-Yan , You Chu-Ming , Meng Qiong , Wang Bin , Zhang Li-Juan , Huang Yu-Ge , Ao Dang , Li Wei-Zhong , Chen Jie-Ling , Chen Yan-Ling , Li Wei , Chen Zhi-Feng , Ding Yue-Qin , Li Xiao-Yu , Huang Yue-Fang , Lin Ni-Yang , Cai Yang-Fan , Wan Zhong-He , Ban Yi , Bai Bo , Li Guang-Hong , Yan Yue-Xiu 
Published: November 2021

Abstract
Background

With the increase in extremely low birth weight (ELBW) infants, their outcomes received special attention. However, in China, studies of the outcomes of ELBW infants are rare.
Methods

The hospitalized records of ELBW infants discharged from twenty-six neonatal intensive care units were reviewed and analyzed.
Results

A total of 2575 ELBW infants were enrolled and the overall survival rate was 55.11%. From 2008 to 2017, the number of ELBW infants increased rapidly from 91 to 466, and the survival rate improved steadily from 41.76–62.02%. The survival rate rose with increasing BW, the ascending level of regional economic development and specialist hospitals. The incidence of complications was neonatal respiratory distress syndrome (85.2%), bronchopulmonary dysplasia (63.7%), retinopathy of prematurity (39.3%), intraventricular hemorrhage (29.4%), necrotizing enterocolitis (12.0%), and periventricular leukomalacia (8.0%). Among the 1156 nonsurvivors, 90.0% of infants died during the neonatal period (≤ 28 days), and the other died after the neonatal period. A total of 768 ELBW infants died after medical care withdrawal, with economic factors and expected outcome being important causes.
Conclusion

The number of ELBW infants is increasing in China, and the overall survival rate is still low but is improving steadily.
Clinicopathological Characteristics of LgG4-Related Lung Disease
Authors: Liu Jia , Liu Yuxiang , Shen Ximing , He Zhanghai , Yu Tingfeng , Pang Li , Jin Xiaoyan , Wang Lingyun 
Published: November 2021

Abstract
Background: Immunoglobulin G4-related lung disease (IgG4-RLD) is a rare entity. The aim of this study is to retrospectively analyzed the clinicopathological characteristics of IgG4-RLD pathologically confirmed in order to improve the diagnosis and treatment of the disease.Methods: We screened the pathological reports of 4838 patients with pulmonary surgery and/or biopsy specimens at Sun Yat-sen Memorial Hospital affiliated to Sun Yat-sen University from April 2017 to April 2021, and 65 patients with suspected IgG4-RLD had an immunohistochemical staining for IgG4 and IgG. Finally, 10 patients with definite IgG4-RLD pathologically comfirmed were enrolled and analyzed.Results: The incidence of IgG4-RLD pathologically confirmed was 0.2% (10/4838). Ten patients had a mean age of 59.7 years at diagnosis with a male: female ratio of 9:1. The initial clinical manifestations were lack of specificity and cough was the most common symptom (4/10). More than one organ was involved in most patients (8/10) and lymph node involvement was often seen (7/10). Serum IgG4 were detected and elevated in 6 patients. The levels of serum tumor markers were within normal range or slightly elevated. The chest computed tomography (CT) and/or 18F-fluorodeoxyglucose positron emission tomography-computed tomography (18F-FDG PET-CT) findings showed 5 patients of mix type, 3 patients of solid nodular type and 2 patients of bronchovascular type. All pulmonary mass and large nodules with solid pattern had spiculated margins and inhomogeneous enhancement with or without pleural indentation and lobulated appearance. Abundant lymphoplasmacytic infiltration and fibrosis were observed in all patients. The expressions of IgG4 and IgG in the plasma cells were up-regulated in the immunostained sections of lung. Seven patients received prednisone with or without additional immunosuppressive drugs and responded well.Conclusions: Our study suggests that multiple imaging findings combined with elevated serum IgG4 as well as serum tumor markers without significant increase could provide diagnostic support for IgG4-RLD.
Genome Wide Association Study of Frost Tolerance in Wheat
Authors: Soleimani Behnaz , Lehnert Heike , Babben Steve , Keilwagen Jens , Koch Michael , Arana-Ceballos Fernando Alberto , Chesnokov Yuriy , Pshenichnikova Tatyana , Schondelmaier Jörg , Ordon Frank , Börner Andreas , Perovic Dragan 
Published: November 2021

Abstract
Winter wheat growing areas in the Northern hemisphere are regularly exposed to heavy frost. Due to the negative impact on yield, the identification of genetic factors controlling frost tolerance (FroT) and development of tools for breeding is of prime importance. Here, we detected QTL associated with FroT by genome wide association studies (GWAS) using a diverse panel of 276 winter wheat genotypes that was phenotyped at five locations in Germany and Russia in three years. The panel was genotyped using the 90K iSelect array and SNPs in FroT candidate genes. In total, 17,566 SNPs were used for GWAS resulting in the identification of 53 markers significantly associated (LOD ≥4) to FroT, corresponding to 23 QTL regions located on 11 chromosomes (1A, 1B, 2A, 2B, 2D, 3A, 3D, 4A, 5A, 5B and 7D). The strongest QTL effect confirmed the importance of chromosome 5A for FroT. In addition, to our best knowledge, seven FroT QTLs were discovered for the first time in this study comprising QTLs on chromosomes 3A, 4A, 1B, and two on chromosomes 2D, 3D, and 7D. Identification of novel FroT candidate genes will help to better understand the FroT mechanism in wheat and to develop more effective combating strategies.
Identifying The Barriers to Universal Cervical Length Screening For Preterm Birth Prevention at Tertiary Hospitals in Thailand—Healthcare Managers’ Perspectives: Implementation Research
Authors: Titapant Vitaya , Chawanpaiboon Saifon , Anuwutnavin Sanitra , Kanjanapongporn Attapol , Pooliam Julaporn , Tangwiwat Pimolphan 
Published: November 2021

Abstract
Objective: To identify healthcare managers’ perspectives on the barriers to implementing cervical length screening to prevent preterm births.Materials and Methods: There were 3 phases of the study. In Phase I, 10 healthcare managers were interviewed. Phase II comprised questionnaire development and data validation. In Phase III, the questionnaire was administered to 40 participants, and responses were analysed.Results: The questionnaire was completed by 40 obstetricians. Their average related work experience was 21.0 ± 7.2 (5–35) years; 39 (97.5%) respondents also had healthcare management responsibilities at their respective hospitals. Most hospitals were reported to have enough obstetricians (31 cases, 77.5%) and to be able to accurately perform cervical length measurements (22 cases, 55.0%). However, no funding was allocated to universal cervical length screening (39 cases, 97.5%). Most respondents believed that implementing universal screening, as per Ministry of Public Health policies, would prevent preterm births (28 cases, 70.0%). Moreover, they suggested that hospital fees for cervical length measurements should be waived (34 cases, 85.0%). Three main perceived barriers to universal screening at tertiary hospitals were identified. They were heavy obstetrician workloads (20 cases, 50.0%); inadequate numbers of medical personnel (24 cases, 60.0%); unbelieving of the screening test to prevent preterm birth (8 cases, 20%) and lack of free drug support for preterm birth prevention in high-risk cases (29 cases, 72.5%).Conclusions: The main obstacles to universal cervical length screening are heavy staff workloads and inadequate government funding for ultrasound scanning and hormone therapy. The healthcare managers do not believe that the universal cervical length screening can help to reduce preterm birth. These barriers could be removed by reducing unnecessary work and providing funding.
Comprehensive targeted next-generation sequencing in patients with slow-flow vascular malformations
Authors: Nozawa Akifumi , Fujino Akihiro , Yuzuriha Shunsuke , Suenobu Souichi , Kato Aiko , Shimizu Fumiaki , Aramaki-Hattori Noriko , Kuniyeda Kanako , Sakaguchi Kazuya , Ohnishi Hidenori , Aoki Yoko , Ozeki Michio 
Published: November 2021

Abstract
Recent studies have shown that the PI3K signaling pathway plays an important role in the pathogenesis of slow-flow vascular malformations (SFVMs). Analysis of genetic mutations has advanced our understanding of the mechanisms involved in SFVM pathogenesis and may identify new therapeutic targets. We screened for somatic variants in a cohort of patients with SFVMs using targeted next-generation sequencing. Targeted next-generation sequencing of 29 candidate genes associated with vascular anomalies or with the PI3K signaling pathway was performed on affected tissues from patients with SFVMs. Fifty-nine patients with SFVMs (venous malformations n = 21, lymphatic malformations n = 27, lymphatic venous malformations n = 1, and Klippel–Trenaunay syndrome n = 10) were included in the study. TEK and PIK3CA were the most commonly mutated genes in the study. We detected eight TEK pathogenic variants in 10 samples (16.9%) and three PIK3CA pathogenic variants in 28 samples (47.5%). We also identified a pathogenic variant in RASA1 in one sample. In total, 38 of 59 patients (64.4%) with SFVMs harbored pathogenic variants in these three genes involved in the PI3K signaling pathway. Inhibitors of this pathway may prove useful as molecular targeted therapies for SFVMs.
GnRHa as a Treatment for Letrozole-Resistent Recurrent Adult Granulosa Cell Tumors: A Case Report and Literature Review
Authors: Yang Hua , Zhang Shushan , Liu Yao , Zhuang Yuan 
Published: November 2021

Abstract
Background: The optimal management of Recurrent Ovarian granulosa cell tumors was still unknown, Hormone therapy maybe an alternative for chemotherapy-resistant cases. the reaction rate for aromatase inhibitors was highest, how to treat the progressed case after aromatase inhibitors was challenging. Case presentation: Here we report a case of Recurrent Ovarian granulosa cell tumors treated with Diphereline and achieved clinical cure. A 46-year-old woman presented with third recurrence after primary treatment. She developed tumor progression and drug-induced nephritis after 6 cycles of combined treatment of cisplatin and paclitaxel for the second recurrence and failed to benefit from chemotherapy ,after the third Optimal cytoreduction and tumor progression after 6 months Letrozole treatent. The implementation of experimental treatment with Diphereline achieved Good therapeutic effect.Conclusion: Hormone therapy maybe an alternative to recurrent granulosa cell tumors, Gonadotropin-releasing hormone agonistsas maybe a rescued treatment for Aroatase inhibitor-resistant cases
Corneal Hysteresis and Intraocular Pressure are Altered in Silicone-Hydrogel Soft Contact Lenses Wearers
Authors: Marcellán María Concepción , Remón Laura , Avila Francisco 
Published: November 2021

Abstract
Purpose: The aim of this work is to determinate the effects in the physical parameters in terms of intraocular pressure (IOP) and central corneal thickness (CCT) and corneal biomechanics in terms of corneal resistance factor (CRF) and corneal hysteresis (CH) of wearing silicone-hydrogel soft contact lenses (SiH-CLs) in young adult subjects during a short-term follow-up. Methods: 40 eyes of 20 healthy patients with a mean age of 22.87±4.14 were involved in this study. Subjects with corneal diseases, dry eye, irregular astigmatism or who have been previous contact lens wearers were excluded. The Ocular Response Analyzer (Reichert Ophthalmic Instruments) was used to measure CH, CRF and IOP and Scheimpflug imaging (Galilei Dual Scheimpflug camera analyzer, Pentacam) was used to measure CCT before and 10 days (Group 1) and 20 days (Group 2) after using the SiH-CLs. Results: IOP was decreased significantly 10 days after using the SiH-CLs (p=0.009). Within the 20 days' period, Group 2 revealed an even more pronounced decrease in IOP (p=.003) and CH increased significantly (p=.04). CCT and CRF did not show a significant change during the period of SiH-CLs use. Our finding allowed obtaining an analytical expression that relates IOP, CCT, CRF and CH within a biomechanical compensation experimental model. Conclusions: Corneal biomechanical parameters and physical properties of the cornea may alter with SiH-CLs use. Our finding could have an impact on the management of glaucoma progression and ocular hypertension.
Incidence and Outcome of Reintubation in the Postanesthesia Care Unit: a Single-center, Retrospective, Observational Matched Cohort Study in China
Authors: Liu Shangkun , Dong Ying , Wan Li , Luo Ailin , Chen Hong , Xu Hui 
Published: November 2021

Abstract
Backgroundː Reintubation after planned extubation (RAP) is a serious adverse event. This study aimed to determine the rate and impact of RAP on postoperative course and hospitalization outcomes.Methodsː Of the 121,965 patients in the PACU, 14 patients with RAP were included in this retrospective, single-center, 1:2 matched cohort study in China from January 1, 2017 to December 31, 2019. Duration of PACU stay, postoperative time in the hospital, inpatient healthcare costs, and outcome of hospitalization were compared between the RAP and matched groups.Resultsː The rate of RAP occurrence was 0.0115%. After propensity score matching, there were no statistically significant differences in age, sex, body mass index, emergency operation, surgical classification, American Society of Anesthesiologists physical status, and the durations of anesthesia and the procedure between the two groups. Duration of PACU stay, length of postoperative stay, and inpatient healthcare costs significantly differed between the RAP and matched groups (P < 0.01 for all). The percentage of patients in whom discharge from the PACU was prolonged was significantly higher in the RAP group than in the matched group (92.86% vs.714%), with an odds ratio of 29.87 (95% CI = 14.00 to 2040.54; P < 0.001) after matching.Conclusionsː RAP in the PACU had low incidence but was associated with life-threatening and serious complications, increased incidence of prolonged discharge, prolonged PACU and postoperative hospital stay, increased inpatient healthcare costs, and unanticipated intensive care unit admission. Hypoxemia, airway obstruction, dyspnea, respiratory depression, confusion, asthma, chest tightness, pain, hemorrhage, or agitation could be the risk factor for RAP. The appropriate time for tracheal extubation and monitoring in PACU can effectively prevent the occurrence of reintubation and improve the prognosis of patients.
The Ambiguous "Internal Carotid Artery" - Ultrasound Diagnosis of One Case of Congenital Absence of The Internal Carotid Artery
Authors: Zhong Gang , Ning Jing , Xie Juan-Juan , Liu Fang , Huang Meng-Yi , Long Xiang-Dang 
Published: November 2021

Abstract
Background
Congenital absence of the internal carotid artery (ICA) is a very rare congenital anomaly of the ICA. Most reports in the literature are of individual cases, and most were diagnosed by computed tomographic angiography (CTA) or digital subtraction angiography (DSA). There are few reports on the diagnosis of congenital absence of the ICA by carotid Doppler ultrasound (CDUS).
Case presentation:
A 61-year-old male patient who was admitted to the hospital because of dizziness and showed no abnormality on CDUS conducted at another hospital. CDUS conducted at our hospital found maldevelopment of the right common carotid artery (RCCA) with normal a blood flow velocity and spectrum. A high-resistance frequency spectrum could be detected in the “ICA” that continued directly from the RCCA, and two adjacent small blood vessel branches were also observed. Superficial temporal artery tap and tracing of the courses of the small blood vessels confirmed that the RCCA directly continued to the external carotid artery (ECA). The final diagnosis was congenital absence of the ICA, which was confirmed during the clipping of an anterior communicating artery (ACoA) aneurysm.
Conclusion
CDUS, as a noninvasive and rapid screening tool for cervical vascular diseases, offers a new approach for the diagnosis of congenital absence of the ICA.
Origin of precious opal revisited: Possible quick formation of precious opal
Authors: Okudera Hiroki , Takeda Tetsuyoshi 
Published: November 2021

Abstract
It has been well known that a sedimentary precious opal is composed of closely packed uni-size silica spheres with voids filled by an air or water, and that an interference among reflections from the boundaries of those spheres and filler yields play-of-colour (iridescence). So, occurrence of a play-of-colour means occurrence of natural selection in size of spheres, or suppression of further nucleation after initial outburst of spheres, during its formation process. We had been exploring the possibility if we can regard a Stöber process as an analogue of the formation process of precious opal. The key is the reason why variation in size is rarely found on both precious opal and Stöber colloid. To give a clue, we examined the internal structure of Stöber particles and how those particles were formed at very initial stage of the process. The answers for evenness in shape and size are a quick supersaturation of reactive silica species, consecutive formation of large and loose polymers by fast dehydration, and their quick aggregation as the initial burst of silica spheres in highly diffusive medium. These can be achieved in nature by quick but continuous decrease in temperature on “basic” (high pH) geothermal hot water moving upward through cracks in rocks. Sedimentary precious opal can thus be formed when such naturally occurring colloid is filtered by a permeable bed.
Cervical Discopathy in Idiopathic Trigeminal Neuralgia: More than Coincidence?
Authors: Börü Ülkü Türk , Bölük Cem , Özdemir Adnan , Demirbaş Hayri , Taşdemir Mustafa , Karabacak Tuğçe Gezer , Şahbaz Fatma Gülhan , Dumanlı Ahmet 
Published: November 2021

Abstract
BackgroundThe most common cause of trigeminal neuralgia (TN) is neurovascular compression. However, a number of patients present with unknown etiology. This study aims to investigate the relationship between TN and cervical pathology in patients previously diagnosed with idiopathic TN. MethodsWe designed an observational case-control study. A study group consisting of patients previously diagnosed with idiopathic TN and a control group was included in the study. Cranial MRI’s of TN patients were re-evaluated by a blinded neuroradiologist. Once it was confirmed that no signs of neurovascular compression or any secondary causes were present, a cervical MRI was performed to evaluate cervical pathologies. Results20 patients and 20 controls were investigated. The mean age of TN patients was 64.9±12.6, and the mean age of the control group was 61.3±9.1 (p=0.305). Whilst indentation on the trigeminal spinal tract above C4 spinal level was observed in 12 out of 20 patients, none of the controls had any involvement in the same region (p<0.001). ConclusionsThe results of this study suggest that extramedullary indentation on the trigeminal spinal tract caused by upper cervical discopathy may be one of the possible etiological factors in TN.