bluuebunny/crossref_metadata_2025_split huggingface dataset:
Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly
Authors: Jiang Xueer , Ho Dac Bang Tam , Mahe Karan , Mia Jennielee , Sepulveda Guadalupe , Antkowiak Mark , Jiang Linhao , Yamada Soichiro , Jao Li-En 
Published: June 2021

ABSTRACT
At the onset of mitosis, centrosomes expand the pericentriolar material (PCM) to maximize their microtubule-organizing activity. This step, termed centrosome maturation, ensures proper spindle organization and faithful chromosome segregation. However, as the centrosome expands, how PCM proteins are recruited and held together without membrane enclosure remains elusive. We found that endogenously expressed pericentrin (PCNT), a conserved PCM scaffold protein, condenses into dynamic granules during late G2/early mitosis before incorporating into mitotic centrosomes. Furthermore, the N-terminal portion of PCNT, enriched with conserved coiled-coils (CCs) and low-complexity regions (LCRs), phase separates into dynamic condensates that selectively recruit PCM proteins and nucleate microtubules in cells. We propose that CCs and LCRs, two prevalent sequence features in the centrosomal proteome, are preserved under evolutionary pressure in part to mediate liquid-liquid phase separation, a process that bestows upon the centrosome distinct properties critical for its assembly and functions.
Ana1 helps recruit Polo to centrioles to promote mitotic PCM assembly and centriole elongation
Authors: Alvarez-Rodrigo Ines , Wainman Alan , Saurya Saroj , Raff Jordan W. 
Published: June 2021

ABSTRACTPolo kinase (PLK1 in mammals) is a master cell cycle regulator that is recruited to various subcellular structures, often by its polo-box domain (PBD), which binds to phosphorylated S-pS/pT motifs. Polo/PLK1 kinases have multiple functions at centrioles and centrosomes, and we have previously shown that in Drosophila phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the pericentriolar material (PCM) that assembles around mother centrioles in mitosis. Here, we show that Ana1 (Cep295 in humans) also helps to recruit Polo to mother centrioles in Drosophila. If Ana1-dependent Polo recruitment is impaired, mother centrioles can still duplicate, disengage from their daughters and form functional cilia, but they can no longer efficiently assemble mitotic PCM or elongate during G2. We conclude that Ana1 helps recruit Polo to mother centrioles to specifically promote mitotic centrosome assembly and centriole elongation in G2, but not centriole duplication, centriole disengagement or cilia assembly.This article has an associated First Person interview with the first author of the paper.
Rückstände in Kochgefässen
Authors: Keller Christine , Gerber Yvonne , Stern Willem B. 
Published: June 2021

Die chemische Analyse von Rückständen in spätmittelalterlichen Kochgefässen hat ergeben, dass es sich hierbei um zermahlene und kalzinierte Knochen, also um Knochenasche handelt. Obschon solche Rückstände in Gefässen vom optischen Erscheinungsbild her vielerorts bekannt sind, stehen wir hinsichtlich ihrer Funktion und Bedeutung vor vielen Fragen.
Diese sonderbare Innenbeschichtung kommt sowohl auf unglasierter wie auch auf glasierter Keramik vor. Da sie über der Glasur liegt, kann es sich nicht um eine Engobe handeln. Wurden in privaten Haushaltungen Knochen verwertet, wofür man Kochtöpfe zweckentfremdet hat, oder hat man Knochenasche als zusätzliche Schutzschicht - vergleichbar mit der modernen Teflonbeschichtung - in den Kochgefässen aufgetragen?
Tätigkeitsbericht der Archäologischen Bodenforschung Basel-Stadt für das Jahr 1999
Authors: Schwarz Peter-A. 
Published: June 2021

Der Tätigkeitsbericht des Kantonsarchäologen bietet einen Überblick über die wichtigsten Kennzahlen und die Arbeiten in den verschiedenen Abteilungen der Archäologischen Bodenforschung im Jahr 1999.
Mit Beiträgen zur Fundchronik im Jahr 1999.
Tüpfelplatten vom Titelberg
Authors: Gerber Yvonne , Burkhardt Andreas , Helmig Guido 
Published: June 2021

Im Rahmen des in den 90er Jahren gross angelegten Projektes zur Untersuchung der keltischen Münzen aus Basel wurde nicht nur nach der chronologischen und typologischen Differenzierbarkeit dieser Fundgattung aufgrund ihrer optischen und physischen Beschaffenheit geforscht, sondern es wurde auch der Frage nachgegangen, mit weichen Münztechniken die Kelten ihr Geld überhaupt hergestellt hatten.
Tüpfelplatten sind von der Forschung früh mit der Produktion von Münzen in Zusammenhang gebracht worden, und zwar sowohl mit gegossenen Potin- als auch mit geprägten Buntmetall- und Edelmetallmünzen.
Bei den Untersuchungen der Fundgattung Tüpfelplatten, die im folgenden Aufsatz dargelegt werden, drehten sich die Fragen um die Herstellung von Metallrohlingen als Grundlage für zu prägende Münzen. 75 Fragmente von Tüpfelplatten vom Oppidum auf dem Titelberg in Luxemburg konnten im Rahmen dieses Projekts archäometrisch untersucht werden.
Erfahrungen beim Einsatz von TachyCAD und Photoplan auf der Grabung Münsterplatz 1 +2
Authors: Schön Udo 
Published: June 2021

Vom Frühjahr 2002 bis zu Beginn von 2003 untersuchten Mitarbeitende der Archäologischen Bodenforschung und der Basler Denkmalpflege die umfangreichen und gut erhaltenen Baustrukturen der spätgotischen Sankt-Johannes-Kapelle in den Liegenschaften Münsterplatz 1 und 2 auf dem Basler Münsterhügel.
Bei den Untersuchungen wurde während zweier Monate erstmalig der Einsatz von TachyCAD und Photoplan getestet.
Experimental-analytical approach to assessing mechanosensitive cartilage blood marker kinetics in healthy adults: dose-response relationship and interrelationship of nine candidate markers
Authors: Herger Simon , Vach Werner , Liphardt Anna-Maria , Nüesch Corina , Egloff Christian , Mündermann Annegret 
Published: June 2021

Purpose: To determine the suitability of selected blood biomarkers of articular cartilage as mechanosensitive markers and to investigate the dose-response relationship between ambulatory load magnitude and marker kinetics in response to load. Methods: Serum samples were collected from 24 healthy volunteers before and at three time points after a 30-minute walking stress test performed on three test days. In each experimental session, one of three ambulatory loads was applied: 100% body weight (BW); 80%BW; 120%BW. Serum concentrations of COMP, MMP-3, MMP-9, ADAMTS-4, PRG-4, CPII, C2C and IL-6 were assessed using commercial enzyme-linked immunosorbent assays. A two-stage analytical approach was used to determine the suitability of a biomarker by testing the response to the stress test (criterion I) and the dose-response relationship between ambulatory load magnitude and biomarker kinetics (criterion II). Results. COMP, MMP-3 and IL-6 at all three time points after, MMP-9 at 30 and 60 minutes after, and ADAMTS-4 and CPII at immediately after the stress test showed an average response to load or an inter-individual variation in response to load of up to 25% of pre-test levels. The relation to load magnitude on average or an inter-individual variation in this relationship was up to 8% from load level to load level. There was a positive correlation for the slopes of the change-load relationship between COMP and MMP-3, and a negative correlation for the slopes between COMP, MMP-3 and IL-6 with MMP-9, and COMP with IL6. Conclusions: COMP, MMP-3, IL-6, MMP-9, and ADAMTS-4 warrant further investigation in the context of articular cartilage mechanosensitivity and its role in joint degeneration and OA. While COMP seems to be able to reflect a rapid response, MMP-3 seems to reflect a slightly longer lasting, but probably also more distinct response. MMP-3 showed also the strongest association with the magnitude of load.
Silencing of Carboxypeptidase E expression inhibits proliferation and invasion of Panc-1 pancreatic cancer cells
Authors: Lou Hong , Loh Y Peng 
Published: June 2021

Background: Pancreatic cancer is one of the leading cause of cancer-related death globally. The molecular basis of this disease is complex and not fully understood. Previous studies have indicated that carboxypeptidase E (CPE) plays a role in promoting tumorigenesis in many cancer types. Here we have investigated the effect of carboxypeptidase E (CPE), including its isoform, in regulating the proliferation, migration and invasion of Panc-1 cells, a pancreatic cell line. Methods: Panc-1 cells were transfected with CPE siRNA which targets both CPE-wild type and its isoform, or scrambled siRNA, for 24 h and then assayed for proliferation by the MTT and colony formation assays, and migration and invasion by wound healing and matrigel assays, respectively. Results: CPE siRNA treatment of Panc-1 cells down-regulated the expression of CPE mRNA by 94.8%. Silencing of CPE mRNA expression resulted in a significant decrease in proliferation as revealed by the MTT assay and a 62.8% decrease in colony formation. Western blot analysis of expression of Cyclin D1 in Panc-1 cells treated with CPE siRNA showed a decrease of 32.5% compared to scr siRNA treated cells, indicating that CPE regulates proliferation through modulating this cell cycle protein. Additionally, suppression of CPE expression in Panc-1 cells significantly decreased migration and invasion. Conclusions: Our findings indicate that CPE may play an important role in regulating cell proliferation, migration and invasion to promote pancreatic cancer tumorigenesis.
Семиотика культуры и феноменология страха (к постановке проблемы) [The semiotics of culture and the phenomenology of fear]
Authors: Lotman Mihhail 
Published: June 2021

The semiotics of culture and the phenomenology of fear. In the paper fear is treated as semiotical phenomenon. The semiotical speciality of fear is that while being a strong semiotical factor, its semiotical nature is often overshadowed and fear is treated proceeding from the scheme of stimulus-reaction. In the paper fear is analysed in the context of both Peirce's semiotics and Saussure's semiology and it will be demonstrated that these approaches allow to open up different aspects of fear: while in Peircean perspective frightful evokes fear, then proceeding from the Saussure's approach we could say that fear creates the frightful, fear appears to be creative; we could even speak of fear as semiosis.
Intelligent Malware - Trends and Possibilities
Authors: Plucar Jan , Frank Jiří , Walter Daniel , Zelinka Ivan 
Published: June 2021

In recent months and years, with more and more computers and computer systems becoming the target of cyberattacks. These attacks are gaining strength and the sophistication of the approach in terms of how to attack. Attackers and Defenders are increasingly using artificial intelligence methods to maximize the success of their actions. For a successful defence, we must be able to anticipate future threats that may come. For these reasons, our research group is engaged in creating experimental software with artificial intelligence to test the possibilities and capabilities of such malware in the event of its deployment. This software has not only malware capabilities but also antimalware and can be used on both sides. This article introduces the reader to the main principles of our design, which can serve as a future platform for cyber defence systems.
Simulation and experimental research of a high-order Bragg grating
semiconductor laser
Authors: E Siyu , Zhou YinLi , Zhang Xing , Zhang Jianwei , Zeng Yugang , Cui Jinjiang , Liu Yun , Ning Yongqiang , Wang Lijun 
Published: June 2021

In this paper, the influence of the epitaxial structure on distributed
Bragg reflector (DBR) grating characteristics is studied by simulation
analysis. Comparative analysis shows that the symmetrical epitaxial
structure can achieve a lower threshold current and, thus, a higher
power. Based on the simulated structure, a DBR laser based on a
symmetric epitaxial structure was fabricated, and a single
longitudinal mode laser output at

∼

1060

n
m

was obtained. The maximum power was
104.5 mW, and the side mode suppression ratio (SMSR) is 43 dB.
Si-substrate LEDs with multiple superlattice interlayers for beyond 24  Gbps visible light communication
Authors: Hu Fangchen , Chen Shouqing , Li Guoqiang , Zou Peng , Zhang Junwen , Hu Jian , Zhang Jianli , He Zhixue , Yu Shaohua , Jiang Fengyi , Chi Nan 
Published: June 2021

High-speed visible light communication (VLC) using light-emitting diodes (LEDs) is a potential complementary technology for beyond-5G wireless communication networks. The speed of VLC systems significantly depends on the quality of LEDs, and thus various novel LEDs with enhanced VLC performance increasingly emerge. Among them, InGaN/GaN-based LEDs on a Si-substrate are a promising LED transmitter that has enabled VLC data rates beyond 10 Gbps. The optimization on the period number of superlattice interlayer (SL), which is a stress-relief epitaxial layer in a Si-substrate LED, has been demonstrated to be an effective method to improve Si-substrate LED’s luminescence properties. However, this method to improve LED’s VLC properties is barely investigated. Hence, we for the first time experimentally studied the impact of SL period number on VLC performance. Accordingly, we designed and fabricated an integrated 4 × 4 multichromatic Si-substrate wavelength-division-multiplexing LED array chip with optimal SL period number. This chip allows up to 24.25 Gbps/1.2 m VLC transmission using eight wavelengths, which is the highest VLC data rate for an InGaN/GaN LED-based VLC system to the best of our knowledge. Additionally, a record-breaking data rate of 2.02 Gbps over a 20-m VLC link is achieved using a blue Si-substrate LED with the optimal SL period number. These results validate the effectiveness of Si-substrate LEDs for both high-speed and long-distance VLC and pave the way for Si-substrate LED design specially for high-speed VLC applications.
A meta-analysis of cohort studies: Traumatic brain injury and risk of Alzheimer’s Disease
Authors: Zhang Jieyu , Zhang Yongkang , Zou Juntao , Cao Fei 
Published: June 2021

IntroductionRecently, some epidemiological studies have reported that cognitive disorders in elderly people is accelerated with traumatic brain injury. But the causal relationship between traumatic brain injury and AD is still an area of controversy.AimsOur review was conducted to estimate the relation between traumatic brain injury and risk of AD.MethodsAll longitudinal population-based studies comparing incidence of AD between subjects with and without traumatic brain injury from their inception to September 2020 were searched in The Cochrane Library, PubMed, Medline, Embase, Web of Science without restriction of language. The meta-analysis was conducted using Stata software.ResultsA total of 17 studies involving 4289,548 individuals were included. After pooling these 17 studies, subjects with traumatic brain injury had significant higher incidence of AD than those without traumatic brain injury (RR: 1.17, 95% CI: 1.05–1.29). When considering the severity of traumatic brain injury, this elevated risk of AD was still significant comparing subjects with moderate and severe traumatic brain injury and those with no traumatic brain injury (RR: 1.30, 95% CI: 1.01–1.59).ConclusionTraumatic brain injury, especially moderate and severe traumatic brain injury may be associated with increased risk of AD.
L’Art des Frères Quay à la Lumière du Grand Verre
Authors: O'Neill Eithne 
Published: June 2021

L’art scénique raffiné des Frères Quay, en stop-motion et en live, est notoirement mystérieux. Par un évitement des codes du cinéma narratif, leur univers imaginaire, en grande partie fait main, résiste aux tentatives de le définir de manière univoque. À y regarder de plus près, leur monde révèle des éléments formels présents dans les stratégies adoptées par Duchamp pour sa sculpture en verre inachevée, nommée Le Grand Verre. Dans quelle mesure ces œuvres d’art, cinétiques ou statiques, ont-elles une histoire à raconter ? En tant que méthode d’investigation comparative, la question peut contribuer à éclaircir ce que c’est qu’un récit.
A arte dos Irmãos Quay à luz do Grande Vidro
Authors: Fonseca Fabio , O'Neill Eithne 
Published: June 2021

A refinada arte cênica dos irmãos Quay, sendo ela em stop-motion ou ao vivo, é notoriamente misteriosa. Evitando os códigos do cinema narrativo, seu mundo imaginário, em grande parte feito à mão, resiste às tentativas de ser definido de maneira unívoca. Após um olhar mais atento, constata-se que o mundo dos irmãos Quay revela elementos formais presentes nas estratégias adotadas por Duchamp na realização do seu trabalho inacabado – O Grande Vidro. Em que medida é que estas obras de arte, cinéticas ou estáticas, têm uma história para contar? Esta questão, tendo como método a análise comparativa, pode ajudar a esclarecer o que é uma narrativa.
Jaki korporatyzm w Polsce? Rada Dialogu Społecznego w okresie funkcjonowania rządu Beaty Szydło
Authors: Olejnik Maciej 
Published: June 2021

Korporatyzm rozumiany jako system formułowania polityki, w ramach którego rząd, związki zawodowe i organizacje pracodawców wspólnie podejmują i implementują decyzje polityczne w obszarze społeczno-gospodarczym, występuje w wielu państwach Europy Zachodniej. Po 1989 roku w Polsce wykształciła się zdeformowana odmiana tego systemu – PR (Public Relations) korporatyzm. W jego ramach rząd pozoruje negocjacje trójstronne z partnerami społecznymi, aby zwiększyć poziom poparcia politycznego wśród obywateli. W rzeczywistości samodzielnie podejmuje decyzje polityczne.
Celem artykułu było zbadanie czy utworzenie nowego organu korporatystycznego (Rady Dialogu Społecznego), dysponującego znacząco większymi kompetencjami niż poprzednik (Trójstronna Komisja ds. Społeczno-Gospodarczych) doprowadziło do transformacji systemu uprawiania polityki w Polsce z PR korporatyzmu na korporatyzm zachodnioeuropejski.
W artykule poddano analizie porównawczej aktywność Rady w okresie funkcjonowania rządu Beaty Szydło (2015–2017) z działalnością Komisji w latach 2002–2015. Wyniki badań ujawniły, że Rada i Komisja były równie nieefektywnymi organami dialogu społecznego, na forum których nie były podejmowane istotne decyzje polityczne. W artykule wykazano, że zwiększenie uprawnień organu korporatystycznego nie musi powodować poprawy jakości dialogu społecznego. Po 2015 roku PR korporatyzm pozostał dominującym systemem formułowania polityki w Polsce.
Epicureans and the City’s Laws
Authors: Diaco Sara 
Published: June 2021

Abstract
The article discusses the accusation advanced by Plutarch and Cicero, according to which the Epicureans are unjust, as they would break the law to pursue pleasure if certain of impunity, and deals with this criticism by analyzing the Epicurean theory of law and justice and comparing it with friendship. The article argues that, from a doctrinal standpoint, philia has a higher place in the Epicurean’s priorities and a stronger efficacy than positive law in serving the naturally just. It thus concludes that, as Plutarch and Cicero suspected, the Epicurean’s ultimate loyalty does not lie with positive law, in spite of its importance for social order, but rather with the naturally just (dikaion), better secured by friendship.
Justice, Power, and Participatory Socialism: on Piketty’s Capital and Ideology
Authors: O’Neill Martin 
Published: June 2021

Abstract
Thomas Piketty’s Capital and Ideology constitutes a landmark achievement in furthering our understanding of the history of inequality, and presents valuable proposals for constructing a future economic system that would allow us to transcend and move beyond contemporary forms of capitalism. This article discusses Piketty’s conceptions of ideology, property, and ‘inequality regimes’, and analyses his approach to social justice and its relation to the work of John Rawls. I examine how Piketty’s proposals for ‘participatory socialism’ would function not only to redistribute income and wealth, but also to disperse economic power within society, and I discuss the complementary roles of redistribution and predistribution in his proposals, and Piketty’s place in a tradition of egalitarian political economy associated with James Meade and Anthony Atkinson. Having elaborated on Piketty’s account of the relationship between economic policy and ideational change, and his important idea of the ‘desacralization’ of private property, I develop ‘seven theses’ on his proposals for participatory socialism, examining areas in which his approach could be enhanced or extended, so as to create a viable twenty-first century version of democratic socialism.
If Skill is Normative, Then Norms are Everywhere
Authors: Westra Evan , Andrews Kristin 
Published: June 2021

Abstract
Birch sketches out an ingenious account of how the psychology of social norms emerged from individual-level norms of skill. We suggest that these individual-level norms of skill are likely to be much more widespread than Birch suggests, extending deeper into the hominid lineage, across modern great ape species, all the way to distantly related creatures like honeybees. This suggests that there would have been multiple opportunities for social norms to emerge from skill norms in human prehistory.
Immunoregulatory function of Dictyophora echinovolvata spore polysaccharides in immunocompromised mice induced by cyclophosphamide
Authors: Lin Chenqiang , Zhang Hui , Chen Longjun , Fang Yu , Chen Jichen 
Published: June 2021

Abstract
The purpose of this study was to investigate whether the Dictyophora echinovolvata spore polysaccharides (DESP) affect the immunity in immunocompromised mice induced by cyclophosphamide (CTX). The healthy female Kunming mice were randomly divided into six groups, including a normal control (NC) group, a positive control group, a model control (MC) group, and three groups treated with low-, intermediate-, and high-dose polysaccharide, respectively. A series of immunoregulatory properties were determined, including humoral and cellular immunity, immune function, and immune factors of mononuclear macrophages. Compared with NC and MC groups, treatment with DESP significantly increased the spleen index and decreased the thymus index; increased the serum concentrations of immunoglobulin (Ig)A, IgG, IgM, hemolysin, IL-1β, and IL-2; delayed the allergic reaction; and improved the splenic lymphocyte transformation ability; and enhanced the phagocytosis of macrophages and the ability to secrete IL-6, TNF-α, caspase-1, and NO with DESP supplementation. These results indicated that DESP might have a good regulatory effect on CTX-induced immunodeficiency in mice, adjust the body’s immune imbalance, and improve the symptoms of low immunity.
Hybrid lightweight Signcryption scheme for IoT
Authors: Sruthi M. , Rajasekaran Rajkumar 
Published: June 2021

AbstractThe information transmitted in IoT is susceptible to affect the user’s privacy, and hence the information ought to be transmitted securely. The conventional method to assure integrity, confidentiality, and non-repudiation is to first sign the message and then encrypt it. Signcryption is a technique where the signature and the encryption are performed in a single round. The current Signcryption system uses traditional cryptographic approaches that are overloaded for IoT, as it consists of resource-constrained devices and uses the weak session key to encrypt the data. We propose a hybrid Signcryption scheme that employs PRESENT, a lightweight block cipher algorithm to encrypt the data, and the session key is encrypted by ECC. The time taken to signcrypt the proposed Signcryption is better when compared to current Signcryption techniques, as it deploys lightweight cryptography techniques that are devoted to resource-constrained devices.