bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tourism and Guidebooks in the Activities of Academic Staff at the Faculty of Fine Art at Stefan Batory University in Vilnius
Authors: Kania Agnieszka 
Published: April 2021

Stefan Batory University in Vilnius was established in 1919 as a regional entity to carry out scientific research and develop Polish academic education. One of its faculties – the Faculty of Fine Arts (1930–1939) – gathered charismatic and popular artists, historians and architects. Among them, two professors – Juliusz Kłos (1888–1933) and Marian Morelowski – published the guidebooks for Vilnius and Wileńszczyzna region (Wilno. Przewodnik krajoznawczy, 1937; Zarys sztuki wileńskiej z przewodnikiem po zabytkiem między Niemnem a Dźwiną, 1939). The book written by Kłos gained popularity and was often purchased by the tourists who visited Vilnius. The aim of the article is to determine the main methodology, historical approaches and working styles of both authors. Moreover, the paper involves their attitude towards developing tourism, cultural heritage and historical research. The article also involves the main ideas of artistic education professors had and how much tourism was involved in the whole education process for them.
Cambios en el perfil proteico de E. coli O157:H7 frente al tratamiento con Ib-M1 e IONP@Ib-M1
Authors: Ramirez Forero Gloria Smith , Valdivieso Quintero Wilfredo , Ropero Vega Jose Luis , Zafra German , Flórez Castillo Johanna Marcela 
Published: April 2021

Escherichia coli O157:H7 es una bacteria patógena responsable de enfermedades transmitidas por alimentos y reconocida por su capacidad de resistencia a diversos antibióticos; razón por la cual, se generan complicaciones en el tratamiento de infecciones producidas por esta bacteria. El péptido Ib-M1 libre e inmovilizado en nanopartículas magnéticas de óxido de hierro (IONP@Ib-M1) ha surgido como una nueva alternativa antimicrobiana contra E. coli O157:H7 y aislados clínicos de esta bacteria. El mecanismo de acción de Ib-M1 e IONP@Ib-M1 contra E. coli O157:H7 aún es desconocido; por lo tanto, el objetivo de esta investigación fue la identificación del cambio en el perfil de proteínas de E. coli O157:H7 luego del tratamiento con Ib-M1 e IONP@Ib-M1 como primer paso para determinar su mecanismo de acción. Para esto, se llevó a cabo la obtención de proteínas, posteriormente se llevó a cabo una electroforesis bidimensional para finalmente realizar la determinación de la variabilidad de los perfiles proteicos. Una vez obtenidos los perfiles proteicos se llevó a cabo un análisis de varianza (ANOVA). Los resultados muestran la identificación de proteínas expresadas diferencialmente y las cuales se encuentra involucradas en procesos de quimiotaxis, síntesis y mantenimiento de proteínas, procesos redox, crecimiento celular, transporte de aminoácidos e inhibición de traducción.
Retratos da “Geração Digital”: um estudo exploratório sobre o consumo midiático e a sociabilidade em Rede na Infância
Authors: Othon Renata Alves de Albuquerque , Coelho Maria das Graças Pinto 
Published: April 2021

O ideal de “Geração Digital” tem sido amplamente utilizado na mídia e em algumas pesquisas para enaltecer a relação entre os jovens e as tecnologias. Questiona-se, no entanto, até que ponto essa concepção abrange os usos reais das TICs, tendo em mente que o consumo é mediado por uma série de contextos. Em vista disso, este artigo tem como objetivo investigar o consumo midiático por crianças para identificar as práticas e sentidos produzidos por elas, adotando-se como referência o acesso à internet pelo smartphone. Aplica-se um estudo exploratório de caráter qualitativo, construído em torno de uma observação não participante e de entrevistas semiestruturadas com cinco jovens entre 12 e 14 anos. Dentre os relatos, identifica-se a interlocução cada vez mais integrada entre ambiências digitais e físicas, públicas e privadas, mediadas pelo telefone celular e pelos conteúdos on-line. Nessa lógica, as tecnologias demarcam culturas de pares e relações de pertencimento a determinados grupos, além de uma subjetividade produzida a partir do olhar do outro. Desse modo, verifica-se que o entretenimento e a sociabilidade protagonizam os usos sociais dessas mídias e contrariam a generalização presente no imperativo “Geração Digital”.
Arho – The Afar Salt Trade of Northeastern Ethiopia
Authors: Trojer Till Jakob Frederik 
Published: April 2021

“Arho – The Afar Salt Trade of North-eastern Ethiopia” follows the journey of a camel caravan to the salt plains of the Afar Depression. Traditionally the caravans moved from Afar Depression to other parts of Ethiopia and to the Red Sea coastal regions of modern-day Eritrea and Djibouti. For centuries the control, trade and distribution of salt was of primary importance in the articulation of economic, social, and political life of the nomadic communities living along the caravan trails. Since 2010, trucks have slowly replaced the camel caravans. This has led to a steady decline in the trade. The film, relying on strong visual imagery and traditional Afar music, selected by the participant of this project, documents how the decline of this trade affects the daily life of the communities in Berahle district of North-eastern Ethiopia. The documentary is part of PhD project at the Department of Anthropology and Sociology at the School of Oriental and African Studies (SOAS), University London. It is based on 13 months ethnographic research (2017-2018) that combines anthropological theory with ethnographic filmmaking to explore new and creative ways of collaborative research.
Locally Activated Renin-Angiotensin System Associated with TGF-β1 as a Major Factor for Renal Injury Induced by Chronic Inhibition of Nitric Oxide Synthase in Rats
Authors: KASHIWAGI MINORU , SHINOZAKI MICHIYA , HIRAKATA HIDEKI , TAMAKI KIYOSHI , HIRANO TADASHI , TOKUMOTO MASANORI , GOTO HIROSHIGE , OKUDA SEIYA , FUJISHIMA MASATOSHI 
Published: April 2021

Abstract.
Chronic inhibition of nitric oxide synthase (NOS) is known to cause renal parenchymal injury with systemic hypertension. To elucidate the pathogenetic mechanism in renal damage induced by NOS inhibition, N
ω-nitro-L-arginine methyl ester (L-NAME) was given orally for 12 wk in Wistar rats, and the roles of tissue renin-angiotensin system and transforming growth factor-β1 (TGF-β1) were investigated. BP and urinary protein excretion increased significantly in L-NAME rats compared with control rats, and glomerulosclerosis and interstitial fibrosis developed. In L-NAME rats, the cortical tissue levels of angiotensin-converting enzyme activity and angiotensin II were significantly higher than those in control rats. The cortical mRNA expressions of both TGF-β1 and fibronectin were significantly elevated in L-NAME rats. Immunohistochemically, increased expressions of both fibronectin and α-smooth muscle actin were also revealed in L-NAME rats. In L-NAME rats, these histologic injuries and the increased expression of TGF-β1 were equally emeliorated by either angiotensin-converting enzyme inhibitor or angiotensin II type 1 receptor antagonist, but not by hydralazine. In conclusion, the locally activated renin-angiotensin system in connection with the increased TGF-β1 expression is a major pathogenetic feature of renal injury in chronically NOS-inhibited rats.
Characterization and Localization of the Neonatal Fc Receptor in Adult Human Kidney
Authors: HAYMANN JEAN-PHILIPPE , LEVRAUD JEAN-PIERRE , BOUET SANDRINE , KAPPES VINCENT , HAGÈGE JACQUELINE , NGUYEN GENEVIEVE , XU YICHUN , RONDEAU ERIC , SRAER JEAN-DANIEL 
Published: April 2021

Abstract.
The binding of Fc fragments of Ig on glomerular epithelial cells (GEC) was observed previously, but the receptor could not be identified. In immunofluorescence and immunohistochemical studies using normal adult human kidney sections, the presence of the so-called neonatal Fc receptor (FcRn) was demonstrated on GEC as well as in the brush border of proximal tubular cells. FcRn transcripts were also detected on isolated glomeruli by reverse transcription-PCR. Using an immortalized GEC line, the presence of the FcRn was confirmed by flow cytometry, reverse transcription-PCR, Western blotting, and by the pH dependence of the binding of heat-aggregated IgG. Because it is well established that the FcRn is involved in IgG transcytosis, it is hypothesized that the FcRn in the kidney may play a role in the reabsorption of IgG. Ongoing studies should clarify the role of the FcRn as a potential target for immune complexes on GEC and should assess its relevance in physiology and pathology.
High Prevalence and Adverse Effect of Hepatitis C Virus Infection in Type II Diabetic-Related Nephropathy
Authors: SOMA JUN , SAITO TAKAO , TAGUMA YOSHIO , CHIBA SHIGEMI , SATO HIROSHI , SUGIMURA KAZUHIKO , OGAWA SUSUMU , ITO SADAYOSHI 
Published: April 2021

Abstract.
Over a 4-yr period in the northeast region of Japan (Tohoku), 3643 patients for whom a renal biopsy was available were screened. In addition, 2370 biopsied patients for whom hepatitis C virus (HCV) serology was available were evaluated. The prevalence of HCV infection was investigated in the 2370 biopsied patients. The highest prevalence of HCV infection was found in type II diabetic-related glomerulosclerosis (II-DGS) (24 of 123; 19.5%). At renal biopsy, clinical and laboratory findings and histologic parameters were comparable between the HCV-positive and -negative II-DGS groups. After renal biopsy, the decline of renal function reflected by the slope of reciprocal serum creatinine (1/SCr) was significantly greater in the HCV-positive group than in the HCV-negative group (P = 0.001). The log-rank test performed on the renal survival curves showed a significant difference in the two groups (P = 0.019). According to a multiple linear regression analysis adjusted for the effect of age, gender, BP, HbA1c, urinary protein excretion, and histologic parameters as covariates, urinary protein excretion (P = 0.011), severe arteriolar hyalinosis (P = 0.006), and HCV infection (P < 0.001) were significantly associated with 1/SCr slope. Finally, HCV infection was randomly examined in 545 outpatients and inpatients with type II diabetes mellitus who did not undergo renal biopsy. Of these, 56 patients were positive for HCV antibody (10.3%), and their proteinuria was heavier than in 489 HCV-negative patients (P = 0.001). This study reveals that HCV infection is present at a high rate in type II diabetic-related nephropathy and may have an adverse effect on the progression of the disease.
Competências colaborativas à formação do estudante: análise segundo domínios do Interprofessional Education Collaborative Expert Panel
Authors: Figueredo Wilton Nascimento , Veras Renata Meira , Silva Gilberto Tadeu Reis da 
Published: April 2021

OBJETIVO: Analisar, segundo os domínios do Interprofessional Education Collaborative Expert Panel, as competências colaborativas desenvolvidas pelo programa PermanecerSUS à formação dos estudantes de saúde. MÉTODO: Estudo qualitativo, sob a perspectiva da etnografia institucional, realizado em instituições de saúde públicas com estudantes, trabalhadores e usuários do sistema público de saúde. Os dados foram coletados por meio da observação participante e entrevista semiestruturada, e analisados com a análise de conteúdo temática, em consonância aos domínios do Interprofessional Education Collaborative Expert Panel. RESULTADOS: Participaram do estudo 60 pessoas, predominantemente do sexo feminino, divididas igualmente entre as unidades. Constatou-se que os indivíduos com menor idade e inseridos o mais precocemente em projetos interprofissionais, desenvolvem competências colaborativas com mais facilidade do que os trabalhadores dos serviços de saúde já atuantes dos serviços. CONCLUSÃO: O PermanecerSUS favorece aos estudantes ética/valores para a prática profissional, trabalho em equipe e corresponsabilidade. Entretanto, é preciso desenvolver entre os trabalhadores dos serviços de saúde a competência da comunicação interprofissional.
#Ocupaparaná
Authors: Minucelli Conrado Pereda , Santos Jaqueline Aparecida Silva , Dombrowski Osmir 
Published: April 2021

O presente trabalho empreende a reconstituição de uma cronologia dos acontecimentos que cercam o movimento das ocupações de escolas públicas paranaense por estudantes secundarista em outubro de 2016, seguida por uma descrição sucinta da organização interna das ocupações e conclui com a apresentação de algumas reflexões sobre aspectos particularmente relevantes para a análise dos movimentos sociais no século XXI. O trabalho está baseado em observações in loco de ocupações ocorridas nas cidades de Cascavel e Toledo no extremo oeste do estado, complementadas por informações divulgadas pela grande imprensa e por diferentes canais na internet.
Optimal Prosthesis Sizing for Radial Head Arthroplasty
Authors: Bagley Joshua J. , Della Rocca Gregory J. , Smith Matthew J. , Kim H. Mike 
Published: April 2021

» Radial head arthroplasty is a viable surgical option when a radial head fracture cannot be reconstructed. Radial head arthroplasty provides a load-bearing articular structure against the capitellum in unstable fractured elbows.» Studies have emphasized the importance of choosing the correct implant size to replicate the native radial head anatomy, citing various consequences of improperly sized radial head prostheses. Overstuffing of the radiocapitellar joint, or lengthening of the radius, has been extensively studied because of its detrimental effects on elbow biomechanics, but other types of improper sizing also have negative consequences.» In the setting of severe fracture-dislocation or revision surgery, anatomic landmarks that are useful for prosthesis sizing often are missing. Various methods have been described to provide guidance for the accurate sizing of a prosthetic radial head; a retrieved radial head, the proximal edge of the lesser sigmoid notch, the radiocapitellar synovial fold, and the ulnohumeral joint space all represent useful references.» Intraoperative radiographic examination is an important step while assessing implant size, including the height of the prosthetic radial head.» Since no single method is perfect on its own, the surgeon should combine as many reference measures as possible, both before and during the procedure, for accurate prosthesis sizing in order to achieve successful outcomes.
Coexistence of Hainan Plume and Stagnant Slab in the Mantle Transition Zone beneath the South China Sea Spreading Ridge: Constraints from Volcanic Glasses and Seismic Tomography
Authors: Chen Shuang-Shuang , Gao Rui , Wang Zewei , Hou Tong , Liao Jie , Yan Cheng 
Published: April 2021

Abstract
The influence of Hainan mantle plume and subducting recycled oceanic crust beneath the spreading ridge of the South China Sea (SCS) have been widely proposed recently, but still controversial and ambiguous. Here, we present seismic tomographic evidence, new major and trace element, and Pb isotopic compositions of volcanic glasses from one International Ocean Drilling Program drill core (Site U1434) in the SCS spreading ridge. The volcanic glasses are relatively enriched in alkalis and light rare earth elements (LREEs) and depleted in heavy REEs (HREEs), exhibit slightly positive anomalies in Nb, Ta, Zr, and Hf as well as a positive Nb relative to La and Th, and show relatively high 207Pb/206Pb and 208Pb/206Pb isotopic ratios, suggesting ocean island basalt- (OIB-) type and enriched mantle 2- (EM2-) type geochemical features likely related to a mantle plume. These geochemical features are consistent with those of late Cenozoic volcanic rocks in Hainan and surrounding areas associated with a mantle plume, likely providing the influence of Hainan mantle plume beneath the spreading ridge of the SCS. The SCS primary-melt and volcanic glasses indicate that the source mantle involved 18.5% eclogite (dense, recycled oceanic crust from the stagnant subducted slab) and 46.1% garnet pyroxenite (produced by the reaction between the peridotite melt and recycled oceanic crust). The existence of Hainan mantle plume and stagnant subducted slab is further supported by geophysical evidence from a recent three-dimensional P-wave seismic tomographic model.
Typical Characteristics of the Earth’s Magnetic and Gravity Fields Related to Global and Regional Tectonics
Authors: Dobretsov N.L. , Metelkin D.V. , Vasilevskiy A.N. 
Published: April 2021

Abstract
—We present a summary and analysis of current views on the magnetic and gravity fields of the Earth as a reflection of global and regional tectonic processes. The discussion concerns the probable interconnection between the distribution of the geomagnetic field characteristics, gravity anomalies and the manifestations of mantle plume magmatism as the most remarkable geologic indicator of deep geodynamics. We demonstrate that the distribution of the characteristics of the main geomagnetic field has a qualitative similarity to anomalies of the gravity field. Brief variations of the geomagnetic field are due to high-frequency oscillations in the ionosphere, do not affect the general state of the field, and are useless when considering issues of global tectonics. On the contrary, variations with long periodicities, first of all geomagnetic reversals, can be among the main indicators of the evolution of the geodynamo – the heat mechanism controlling the entire series of global tectonic processes. The frequency of reversals is determined by the intensity of mantle plumes that cause the cooling of the core, increase the convection rate in the asthenosphere, and respectively, the periodic changes in the tectonosphere. We assume the existence of three modes of behavior for this system. The first one corresponds to steady convection, in which reversals are extremely rare or do not happen at all. These episodes – superchrons – compose no more than 20% of the duration of the Phanerozoic. The second mode occurs significantly more often in the geologic history and is characterized by active convection with frequent reversals happening at least once every 5 Myr. Finally, the third mode, which is rare for the Phanerozoic but was probably more prevalent in the early Precambrian, corresponds to hyperactive turbulent convection, when the frequency of reversals reached 20 and possibly more during one million years. Although the demonstrated qualitative similarity in the position of extreme values of the main geomagnetic field, the centers of free air gravity anomalies, and manifestations of large igneous provinces does not yet have a credible explanation, we consider it to be fundamental and requiring special study and detailed elaboration.
Massively parallel and time-resolved RNA sequencing in single cells with scNT-Seq
Authors: Qiu Qi , Hu Peng , Wu Hao 
Published: April 2021

Abstract
Single-cell RNA sequencing offers snapshots of whole transcriptomes but obscures the temporal dynamics of RNA biogenesis and decay. Here we present single-cell metabolically labeled new RNA tagging sequencing (scNT-Seq), a method for massively parallel analysis of newly-transcribed and pre-existing RNAs from the same cell. This droplet microfluidics-based method enables high-throughput chemical conversion on barcoded beads, efficiently marking newly-transcribed RNAs with T-to-C substitutions. The steps of the protocol are (1) metabolically labeling of cells with 4sU, (2) co-encapsulating individual cell with a barcoded oligo-dT primer coated bead in a nanoliter-scale droplet, (3) performing one-pot 4sU chemical conversion on pooled barcoded beads, and (4) reverse transcription, cDNA amplification, tagmentation, indexing PCR, and sequencing. scNT-Seq provides a broadly applicable strategy to investigate dynamic biological systems at single-cell resolution.
A Case Study of a Conservation Flagship Species: The Monarch Butterfly
Authors: Preston Stephanie D. , Liao Julia D. , Toombs Theodore P. , Romero-Canyas Rainer , Speiser Julia , Seifert Colleen M. 
Published: April 2021

Abstract
What makes a flagship species effective in engaging conservation donors? Large, charismatic mammals are typically selected as ambassadors, but a few studies suggest butterflies – and monarchs in particular – may be even more appealing. To gather more information about people’s responses to monarchs, we conducted an empirical study of member submissions to a successful conservation campaign, the Monarch Story Campaign, conducted by the Environmental Defense Fund (EDF). The set of 691 stories along with their associated demographic and donation data was analyzed in a mixed-methods study using qualitative analysis and tests of association. The results showed that people often described encounters with monarchs in childhood and as adults. They expressed strong, positive emotions, and lauded the monarch’s beauty and other “awe-inspiring” qualities and expressed wonder at their lifecycle (i.e., metamorphosis and migration). They also raised conservation themes of distress at monarch loss, calls for action, and caretaking, such as being “fragile” and “in need.” Sharing personal encounters was associated with current efforts to save the species and more financial donations in the past, while a second pattern tied more donations to awe at the monarch’s mass migration. These results imply that conservation campaigns built around species people encounter may build lifelong awareness, concern, and actions towards conservation.
Silencing circ_0058063 enhances proliferation and inhibits apoptosis in PCOS ovarian granulosa cells
Authors: Wen Chengcai , Zhang Li 
Published: April 2021

Abstract
Background Polycystic ovary syndrome (PCOS) is the most common endocrine disease in reproductive-aged women. This study was designed to explore the role of circ_0058063 in PCOS.Methods We recruited nine PCOS patients and nine no-PCOS patients. The concentrations of follicle stimulating hormone (FSH), testosterone (T), luteinizing hormone (LH), progesterone (P4) and estradiol (E2) were measured by radioimmunoassay. The level of aromatase was detected using an ELISA kit. The proliferation and apoptosis of ovarian granulosa cells were assessed using CCK-8 assay and flow cytometry, respectively. Gene and protein expression were evaluated through RT-qPCR and Western blotting assay.Results The circ_0058063 level in ovarian granulosa cells and follicular fluid is significantly higher in the PCOS group than the no-PCOS group. Besides, silencing circ_0058063 increases the levels of Aromatase mRNA, P4 and E2 in PCOS ovarian granulosa cells. Additionally, silencing circ_0058063 can promote the proliferation of ovarian granulosa cells in patients with PCOS. Furthermore, silencing circ_0058063 can suppress apoptosis of PCOS ovarian granulosa cells.Conclusions Silencing circ_0058063 enhances proliferation and inhibits apoptosis in PCOS ovarian granulosa cells. Such findings may offer vital insights into a therapeutic target for PCOS.
Preparation of di-boride coatings by electrophoretic deposition in nanoparticle-containing molten inorganic salts
Authors: Jin Weiliang , Xiao Saijun , Kou Qian , Ding Desheng , Zhang Jun , Fang Xiaohong , Ge Chuntao , Zhong Cong , Zhu Hongmin , Haarberg Geir Martin 
Published: April 2021

Abstract
Molten inorganic salts containing solid nanoparticles with a stable and uniform dispersion have attracted great attention as efficient heat transfer and storage materials1,2 and for catalysis for chemical reactions3-5. Electrophoretic deposition in molten inorganic salts containing nanoparticles, have not been reported in the literature, compared with the related wide investigations in aqueous and organic suspensions6,7. Here we report the possibility of electrophoretic deposition of nanoparticles in high-temperature molten salts. In molten fluorides and chlorides, cell voltages of 1.2-1.5 V below the decomposition voltage of the electrolytes, were applied to perform the electrophoretic deposition of nanoparticles (e.g., TiB2 and ZrB2) on different cathode substrates, resulting in compact and adhesive coatings with high hardness. These findings should present opportunities to synthesize additional coatings and films via the proposed process.