bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of Key Genes, Signaling Pathways and miRNAs in Human Knee Osteoarthritis: Based on Bioinformatics
Authors: Chang Liang , Yao Hao , Yao Zhi , Ho Kevin Ki-Wai , Ong Michael Tim-Yun , Dai Bingyang , Tong Wenxue , Xu Jiankun , Qin Ling 
Published: August 2021

Background: Osteoarthritis (OA) is one of the main causes of disability in the elderly population, accompanied by a series of underlying pathologic changes, such as cartilage degradation, synovitis, subchondral bone sclerosis, and meniscus injury. The present study aimed to identify key genes, signaling pathways, and miRNAs in knee OA associated with the entire joint components, and to explain the potential mechanisms using computational analysis.Methods: The differentially expressed genes (DEGs) in cartilage, synovium, subchondral bone, and meniscus were identified using the Gene Expression Omnibus 2R (GEO2R) analysis based on dataset from GSE43923, GSE12021, GSE98918, and GSE51588, respectively and visualized in Volcano Plot. Venn diagram analyses were performed to identify the overlapping DEGs (overlapping DEGs) that expressed in at least two types of tissues mentioned above. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, protein-protein interaction (PPI) analysis, and module analysis were conducted. Furthermore, qRT-PCR was performed to validate above results using our clinical specimens.Results: As a result, a total of 236 overlapping DEGs were identified, of which 160 were upregulated and 76 were downregulated. Through enrichment analysis and constructing the PPI network and miRNA-mRNA network, knee OA-related key genes, such as HEY1, AHR, VEGFA, MYC, and CXCL12 were identified. Clinical validation by qRT-PCR experiments further supported above computational results. In addition, knee OA-related key miRNAs such as miR-101, miR-181a, miR-29, miR-9, and miR-221, and pathways such as Wnt signaling, HIF-1 signaling, PI3K-Akt signaling, and axon guidance pathways were also identified. Among above identified knee OA-related key genes, pathways and miRNAs, genes such as AHR, HEY1, MYC, GAP43, and PTN, pathways like axon guidance, and miRNAs such as miR-17, miR-21, miR-155, miR-185, and miR-1 are lack of research and worthy for future investigation.Conclusion: The present informatic study for the first time provides insight to the potential therapeutic targets of knee OA by comprehensively analyzing the overlapping genes differentially expressed in multiple joint components and their relevant signaling pathways and interactive miRNAs.
Clemastine Ameliorates Perioperative Neurocognitive Disorder in Aged Mice Caused by Anesthesia and Surgery
Authors: Wu Wensi , Zhang Xiaojun , Zhou Jiaxin , Yang Hongmei , Chen Junjun , Zhao Le , Zhong Junying , Lin Wei-jye , Wang Zhi 
Published: August 2021

Perioperative neurocognitive disorder (PND) leads to progressive deterioration of cognitive function, especially in aged patients. Demyelination is closely associated with cognitive dysfunction. However, the relationship between PND and demyelination remains unclear. Here we showed that demyelination was related to the pathogenesis of PND. Clemastine, an antihistamine with potency in remyelination, was predicted to have a potential therapeutic effect on PND by next-generation sequencing and bioinformatics in our previous study. In the present study, it was given at 10 mg/kg per day for 2 weeks to evaluate the effects on PND in aged mice. We found that clemastine ameliorated PND and reduced the expression levels of inflammatory factors such as tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β). Further investigation suggested clemastine increased the expression of oligodendrocyte transcription factor 2 (OLIG2) and myelin basic protein (MBP) to enhance remyelination by inhibiting the overactivation of the WNT/β-catenin pathway. At the same time, the expression of post-synaptic density protein 95 (PSD95, or DLG4), brain-derived neurotrophic factor (BDNF), synaptosomal-associated protein 25 (SNAP25) and neuronal nuclei (NEUN) were also improved. Our results suggested that clemastine might be a therapy for PND caused by anesthetic and surgical factors in aged patients.
The Role of Blood Pressure in Carotid Plaque Incidence: Interactions With Body Mass Index, Age, and Sex-Based on a 7-Years Cohort Study
Authors: Liu Jian , Ma Xuehua , Ren Xue-Ling , Xiao Hong , Yan Leyuan , Li Zhuorong , Wang Shengshu 
Published: August 2021

Background: Although high blood pressure (BP) is a risk factor for carotid plaque, its long-term prognostic value might be underestimated due to its confounding interactions with BMI, age, and gender. Therefore, we conducted a 7-year prospective cohort study to evaluate the prognostic value of BP for the incidence of carotid plaque.Methods: The subjects enrolled in 2011 were free of carotid plaque at baseline and were followed up in 2018. Multivariate Cox proportional-hazards models were used to evaluate the association between BP and carotid plaque incidence.Results: During the follow-up study, the incidence of carotid plaque was 36.5%. The significant positive linear trend showed that subjects with higher BP levels at baseline were more likely to develop carotid plaques at the end. Especially in the female subpopulation, after confounders being adjusted, the carotid plaque was associated with higher BP (adjusted HR 1.52, 95% CI 1.02–2.26), pulse pressure (PP) (adjusted HR 1.15, 95% CI 0.76–1.75), and mean arterial pressure (MAP) (adjusted HR 1.44, 95% CI 1.00–2.08). The adjusted HRs of hypertension, PP, and MAP (HR 27.71, 95% CI 2.27–338.64; HR 14.47, 95% CI 1.53–137.18; HR 9.97, 95% CI 1.29–77.28) were significantly higher after the potential antagonistic interactions between BP categorical indicators and age being adjusted, respectively.Conclusion: High BP indicators might be associated with higher HRs of carotid plaque after adjusting interactions between BP indicators and BMI, age, and gender, which suggests that the incidence of carotid plaque in female adults with high BP indicators might increase significantly with the increase of age.
Ventriculosternal Shunt for the Treatment of Idiopathic Normal Pressure Hydrocephalus: A Case Report
Authors: Guo Xinxia , Popal Abdul Malik , Zhu Zhoule , Cai Chengwei , Lin Jingquan , Jiang Hongjie , Zheng Zhe , Zhang Jianmin , Shao Anwen , Zhu Junming 
Published: August 2021

Background: Conventional corticospinal fluid (CSF) diversion surgery for idiopathic normal pressure hydrocephalus (iNPH) includes ventriculoperitoneal shunt and ventriculoatrial shunt. Ventriculosternal (VS) shunt may be considered if both the abdominal cavity and atrium are not feasible.Methods: A 76-year-old woman was admitted to our hospital with gait disturbance and urinary incontinence for 2 years, and the condition aggravated in the last 1 month. Based on clinical assessment and imaging findings, the patient was diagnosed with iNPH, with surgical indications. She was on peritoneal dialysis for chronic renal failure, and a cardiac Doppler echocardiogram showed enlargement of the left atrium and decreased diastolic function of the left ventricle. Due to these conditions, we chose the sternum as the vessel for CSF absorption and performed VS shunt.Results: No swelling, exudation, and effusion were found in the suprasternal fossa. Gait disturbance and urinary incontinence improved significantly immediately and 1 week after surgery, respectively. No shunt-related complication was reported at 16 months follow-up.Conclusion: This case demonstrated VS shunting as a feasible and alternative for the management of hydrocephalus.
Prevalence and Associated Factors of Common Mental Disorders in Women: A Systematic Review
Authors: Bezerra Héllyda de Souza , Alves Roberta M. , Nunes Aryelly Dayanne d. , Barbosa Isabelle R. 
Published: August 2021

Objectives: To identify the prevalence and factors associated with common mental disorders in adult women.Methods: Searches were carried out in the PubMed, Web of Science, Science Direct, Scopus, Cinahl, Google Scholar and Open Gray databases. The study protocol was registered with PROSPERO under number CRD42020168231. Cross-sectional studies showing the prevalence of common mental disorders in women over 18 years were included. Studies with men, children and pregnant women of another age group and with other mental disorders and other types of studies were excluded. The Joanna Briggs Institute checklist was used to assess the risk of bias.Results: Nineteen studies were included in this review. The prevalence of CMD ranged from 9.6% to 69.3%. The main associated factors were unemployment, indebtedness, low income, being a housewife, smoking, low education, poor self-rated health, being single, divorced or widowed. The risk of bias in the studies was classified as low and moderate.Conclusion: This review revealed a variable prevalence rate of CMD in adult women. Public policies are needed to create strategies to prevent the mental illness of these women.
SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice
Authors: Park Hyun-Ji , Jung Hae-Myeong , Lee Areum , Jo Seung-Hee , Lee Hyo-Jun , Kim Hyun-Soon , Jung Choon-Kyun , Min Sung-Ran , Cho Hye-Sun 
Published: August 2021

OsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the U snRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice.
Nomofobia
Authors: Luy-Montejo Carlos , Medina Gamero Aldo , Garcés Lopez Jonathan , Tolentino Escarcena Rodolfo Eloy 
Published: August 2021

El presente estudio se refiere al tema de la nomofobia en estudiantes universitarios, que se puede definir como el miedo irracional que muestra el estudiante al no usar su celular de manera constante y hasta innecesaria. Por consiguiente, adquiere distintos temores como no recibir o emitir mensajes, no estar conectado con sus amigos, aun estando con ellos en un mismo lugar, o estar conectado en alguna aplicación social de su teléfono inteligente
La característica principal de una persona nomofóbica, además del temor constante, es el aislamiento personal que disminuye el contacto social, al mismo tiempo se le asigna problemas fisiológicos o psicológicos que también van de la mano.
El estudio de esta problemática social-académica se realizó por el interés de conocer la razón del crecimiento de este gran grupo de personas con esta patología tecnológica, las causas y la relación con su rendimiento académico.
El desarrollo de este estudio tiene como objetivo analizar las causas principales de la nomofobia en estudiantes universitarios; así también poder describir las causas sociales que estimulan en los jóvenes universitarios este miedo irracional a no tener el celular denominado nomofobia y, finalmente, se busca identificar las causas personales de los estudiantes mencionados que padecen de esta patología. Se utilizará como metodología una revisión sistemática de fuentes, tanto internacionales como nacionales ligados a esta problemática que está cada vez más difundida entre nuestros jóvenes universitarios.
Effect of substrate temperature on plasma-enhanced self-assembling formation of high-density FePt nanodots
Authors: Honda Shunsuke , Makihara Katsunori , Taoka Noriyuki , Furuhata Hiroshi , Ohta Akio , Oshima Daiki , Kato Takeshi , Miyazaki Seiichi 
Published: August 2021

Abstract
We formed FePt magnetic nanodots (NDs) by exposing an ultrathin bilayer metal stack on ∼3.0 nm SiO2/Si(100) substrates to a remote H2 plasma (H2-RP) and studied the effect of external heating during the exposure to H2-RP on the formation and magnetic properties of NDs. The ultrathin bilayer with a uniform surface coverage drastically changed to NDs with an areal density as high as ∼3.5 × 1011 cm−2 by exposing to H2-RP with external heating. We also found that NDs formed by the exposure to H2-RP at 400 °C exhibited a perpendicular anisotropy with a perpendicular coercivity of ∼1.5 kOe, reflecting the magneto-crystalline anisotropy of (001)-oriented L10 phase FePt.
SOSIALISASI DAN PENDAMPINGAN DALAM PENERAPAN SISTEM INFORMASI LAYANAN PERPUSTAKAAN KAB KARAWANG
Authors: Tukino  , Faqih Pratama Muthi  , Aditia Agustian  
Published: August 2021

Kegiatan Layanan Perpustakaan Karawang dibuka mulai jam 08.00 pagi sampai dengan jam
17.00 sore. Perpustakaan Karawang merupakan bentuk perpustakaan yang milik Pemda Kabupaten Karawang yang dipergunakan untuk umum baik masyarakat Karawang maupun masyarakat dari luar Karawang. Sistem informasi layanan Perpustakaan dapat memudahkan bagi pengunjung atau anggota untuk mencari referensi sebagai sumber bacaan. Keberadakan Sistem informasi layanan perpustakaan perlu diperkenalkan kepada masyarakat setempat, agar animo masyarakat untuk mengunjungi perpustakaan semakin meningkat sehingga terbentuk budaya gemar membaca.

Melalui LPPM dan Program Studi Sistem Informasi mengadakan penelitian terhadap penerapan tatakelelo teknologi informasi dalam bidang layanan perpustakaan Kab. Karawang. Setelah dilakukan analisa, bahwa Perpustakaan Kabupaten Karawang membutuhkan sebuah Sistem Informasi untuk memberikan layanan yang optimal bagi seluruh stakeholder.

Pembuatan Sistem Informasi Layanan Perpustakaan Kab Karawang dapat diselesaikan tepat waktu dan dapat digunakan sebaik mungkin. Sebelum Sistem Informasi digunakan secara resmi, perlu dilakukan sosialisasi terhadap pegawai yang nantinya bertanggungjawab dalam pengoperasian dan bagi anggota perpustakaan yang akan menggunakan sistem informasi tersebut. Setelah dilakukan sosialisasi, dilanjutkan dengan Program pendampingan selama uji coba sistem.

Kegiatan Pengabdian kepada Masyarakat memberikan dampak yang positif dalam pengembangan tata kelola teknologi informasi yaitu : 1) Terlaksananya layanan perpustakaan secara komputerisasi, 2) Masyarakat dapat mengakses secara langsung koleksi buku dengan cepat dan mudah, 3) Memberikan daya tarik bagi masyarakat untuk mengunjungi perpustakaan dan 4) Meningkatkan kinerja staf perpustakaan

Kata Kunci : Penerapan, Sistem Informasi Layanan Perpustakan, Peminjaman Buku.
Discovery of a Low-mass Comoving System Using NOIRLab Source Catalog DR2
Authors: Kiwy Frank , Faherty Jacqueline , Meisner Aaron , Schneider Adam C. , Kuchner Marc , Kirkpatrick J. Davy ,   The Backyard Worlds: Planet 9 Collaboration
Published: August 2021

Abstract
We present the discovery of a low-mass comoving system found by means of the NOIRLab Source Catalog DR2. The system consists of the high proper-motion star LEHPM 5005 and an ultracool companion 2MASS J22410186-4500298 with an estimated spectral type of L2. The primary (LEHPM 5005) is likely a mid-M dwarf but over-luminous for its color, indicating a possible close equal mass binary. According to the Gaia EDR3 parallax of the primary, the system is located at a distance of 58 ± 2 pc. We calculated an angular separation of 7.″2 between both components, resulting in a projected physical separation of 418 au.
The Effect of Tiwari Onion (Eleutherine americana Merr) Tablet on Blood Pressure Stability in Diagnosed Hypertension Patients
Authors: Warastuti Widya 
Published: August 2021

BACKGROUND: Non-pharmacological management is carried out by adjusting the lifestyle and non-pharmacological therapy for hypertension sufferers. They use herbal therapy, which is believed to have low side effects, easy, and inexpensive, such as Eleutherine americana Merr Tea.

AIM: This study aimed to analyze Effect of E. americana Merr Tablet on blood pressure in hypertensive patients to be used as supportive therapy to reduce and stabilize blood pressure.

METHODS: This study used a quasi-experimental research design pre-post-test with a control group design, involving 30 respondents. Data collection was carried out for 1 month. Then blood pressure observations were carried out every 1 week for a month after giving E. americana tablets. The sampling technique was purposive sampling method. The data analysis used was the Independent Sample T-Test with a significance level of p < 0.05.

RESULTS: The majority of respondents according to age were in the final elderly age range; 15 people (50%) were 56–65 years old. Most of the respondents involved were female, about 18 people (60%). The respondents’ hypertension category included hypertension Grade 1 (57%) and hypertension Grade II (43%). The independent sample t-test obtained p = systole was 0.029 and diastole was 0.000 (p < 0.005). It showed a significant difference in blood pressure before and after being given E. americana tablets in hypertensive patients.

CONCLUSION: Significant decrease in systolic and diastolic blood pressure in patients with suspected hypertension who obtained E. americana tablets.
The Epidemiology of Knee Arthroscopy during Coronavirus Disease 2019 Pandemic
Authors: Santoso Asep , Anwar Iwan Budiwan , Sibarani Tangkas SMHS , Utomo Pamudji , Arimukti Zarkasyi , Yudistiro Ibnu , Hamid Muhammad Abdul 
Published: August 2021

Introduction: Coronavirus disease (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus (SARS Cov-2) has made a huge impact on global health service including in orthopaedic surgical service. Knee surgery (including arthroscopy) is one of the emerging surgical services in Indonesia. As the pandemic goes on, some of the patients prefer to postpone or cancel the surgery planning. We tried to evaluate the epidemiology of knee arthroscopy procedure during Covid-19 pandemic at our institution.
Material and methods: Retrospective data collection was performed on all patients who received knee arthroscopy procedure at Prof dr. R. Soeharso Orthopaedic Hospital, Surakarta, Indonesia during the year 2020. The study evaluated the demographic data, the indication of surgery, and trends of the number of surgeries according to period time. This study also compared the data of knee arthroscopy procedures performed during the corresponding period before the pandemic in Indonesia (January to December 2019).
Results: There was a total of 88 knee arthroscopy surgery performed during the period January to December 2020. There was a decrease in the number of knee arthroscopy during April and May 2020. The decreasing number of surgeries also occurred in November 2020 which was later followed by the increasing number of surgeries in December 2020. Among all patient received knee arthroscopy during 2020, none have post-operative complication or infection due to SARS Cov-2.
Conclusion: There was some difference in the epidemiology of knee arthroscopy during the COVID-19 pandemic compared to the non-pandemic era. Comprehensive preoperative screening is needed before resume elective surgical service.
Prioritization of Vulnerable Species Under Scenarios of Anthropogenic-Driven Change in Georgia's Coastal Plain
Authors: Paulukonis Elizabeth A. , Crawford Brian A. , Maerz John C. , Wenger Seth J. , Nibbelink Nate P. 
Published: August 2021

AbstractEffective management of wildlife populations benefits from an understanding of the long-term vulnerability of species to anthropogenic stressors. Exposure to potential habitat change is one measure of vulnerability that wildlife managers often use to assess and prioritize individual species or groups of species for resource allocation or direct management actions. We used species distribution models for 15 species occurring in the coastal plain ecoregion of Georgia to estimate the current amount and distribution of potential habitat and then predict exposure to changes in habitat due to inundation from sea level rise (using the Sea Level Affecting Marshes model) and urban growth (using the Slope Land-use Excluded Urban Topology Hillshade Growth model) for four future time points. Our results predict that all focal species were likely to experience some exposure to habitat change from either sea level rise or urbanization, but few species will experience high exposure to change from both stressors. Species that use salt marsh or beach habitats had the highest predicted exposure from sea level rise (25–69%), while species that use more inland habitats had the highest predicted exposure to urban growth (10–20%). Our models are a resource for managers considering tradeoffs between prioritization schemes under two future stressors. Results suggest that managers may need to prioritize species (or their habitats) based on the predicted magnitude of habitat loss, while also contextualizing prioritization with respect to the current amount of available protected habitat and species global vulnerability.
Purinergic Receptor P2Y6 Is a Negative Regulator of NK Cell Maturation and Function
Authors: Li Zhenlong , Gao Yaoxin , He Cong , Wei Huan , Zhang Jiang , Zhang Hongmei , Hu Lulu , Jiang Wenzheng 
Published: August 2021

Abstract
NK cells are critical innate immune cells that target the tumor cells and cancer-initiating cells and clear viruses by producing cytokines and cytotoxic granules. However, the role of the purinergic receptor P2Y6 in the NK cells remains largely unknown. In this study, we discovered that the expression of P2Y6 was decreased upon the activation of the NK cells. Moreover, in the P2Y6-deficient mice, we found that the deficiency of P2Y6 promoted the development of the NK precursor cells into immature NK and mature NK cells. We also found that the P2Y6 deficiency increased, but the P2Y6 receptor agonist UDP or UDP analog 5-OMe-UDP decreased the production of IFN-γ in the activated NK cells. Furthermore, we demonstrated that the P2Y6-deficient NK cells exhibited stronger cytotoxicity in vitro and antimetastatic effects in vivo. Mechanistically, P2Y6 deletion promoted the expression of T-bet (encoded by Tbx21), with or without the stimulation of IL-15. In the absence of P2Y6, the levels of phospho-serine/threonine kinase and pS6 in the NK cells were significantly increased upon the stimulation of IL-15. Collectively, we demonstrated that the P2Y6 receptor acted as a negative regulator of the NK cell function and inhibited the maturation and antitumor activities of the NK cells. Therefore, inhibition of the P2Y6 receptor increases the antitumor activities of the NK cells, which may aid in the design of innovative strategies to improve NK cell–based cancer therapy.