bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue-specific regulation of epidermal contraction duringCaenorhabditis elegansembryonic morphogenesis
Authors: Drewnik Elizabeth D , Wiesenfahrt Tobias , Smit Ryan B , Park Ye-Jean , Pallotto Linda M , Mains Paul E 
Published: May 2021

AbstractActin and myosin mediate the epidermal cell contractions that elongate the Caenorhabditis elegans embryo from an ovoid to a tubular-shaped worm. Contraction occurs mainly in the lateral epidermal cells, while the dorsoventral epidermis plays a more passive role. Two parallel pathways trigger actinomyosin contraction, one mediated by LET-502/Rho kinase and the other by PAK-1/p21 activated kinase. A number of genes mediating morphogenesis have been shown to be sufficient when expressed either laterally or dorsoventrally. Additional genes show either lateral or dorsoventral phenotypes. This led us to a model where contractile genes have discrete functions in one or the other cell type. We tested this by examining several genes for either lateral or dorsoventral sufficiency. LET-502 expression in the lateral cells was sufficient to drive elongation. MEL-11/Myosin phosphatase, which antagonizes contraction, and PAK-1 were expected to function dorsoventrally, but we could not detect tissue-specific sufficiency. Double mutants of lethal alleles predicted to decrease lateral contraction with those thought to increase dorsoventral force were previously shown to be viable. We hypothesized that these mutant combinations shifted the contractile force from the lateral to the dorsoventral cells and so the embryos would elongate with less lateral cell contraction. This was tested by examining 10 single and double mutant strains. In most cases, elongation proceeded without a noticeable alteration in lateral contraction. We suggest that many embryonic elongation genes likely act in both lateral and dorsoventral cells, even though they may have their primary focus in one or the other cell type.
An age-downregulated ribosomal RpS28 protein variant regulates the muscle proteome
Authors: Jiao Jianqin , Kavdia Kanisha , Pagala Vishwajeeth , Palmer Lance , Finkelstein David , Fan Yiping , Peng Junmin , Demontis Fabio 
Published: May 2021

Abstract
Recent evidence indicates that the composition of the ribosome is heterogeneous and that multiple types of specialized ribosomes regulate the synthesis of specific protein subsets. In Drosophila, we find that expression of the ribosomal RpS28 protein variants RpS28a and RpS28-like preferentially occurs in the germline, a tissue resistant to aging and that it significantly declines in skeletal muscle during aging. Muscle-specific overexpression of RpS28a at levels similar to those seen in the germline decreases early mortality and promotes the synthesis of a subset of proteins with known anti-aging roles, some of which have preferential expression in the germline. These findings indicate a contribution of specialized ribosomal proteins to the regulation of the muscle proteome during aging.
Network-Exposure Severity and Self-Protective Behaviors: The Case of COVID-19
Authors: Litwin Howard , Levinsky Michal 
Published: May 2021

Abstract

Background and Objectives
To clarify whether awareness of the extent and severity of exposure to the coronavirus disease 2019 (COVID-19) in the social networks of older adults is related to the engagement by the latter in self-protective behaviors. The inquiry is guided by the Health Belief Model and by concepts from the domain of social networks.

Research Design and Methods
Data from the Survey of Health, Ageing and Retirement in Europe (SHARE) were used, including the SHARE COVID-19 Survey executed in the summer of 2020. The study sample numbered 33,053 persons aged 50 and older in 26 countries. We regressed a logged count of self-protective behaviors on network-exposure severity, controlling for sociodemographic background, country, personality traits, and self-exposure severity. Age and network-exposure interaction terms were examined, as were “close family” and “other” network ties.

Results
Network-exposure severity was positively associated with the extent of engagement in self-protective behaviors among older adults, but mainly among the oldest group, aged 70 and older. Awareness of exposure severity in “close family” and “other” networks were similarly associated with self-protection. Respondents from countries with the lowest rates of COVID-19 infection at the time (Latvia, Finland, and Denmark) engaged in fewer self-protective behaviors, while those from countries with high infection rates (Spain, Italy, and Portugal) self-protected to a greater degree.

Discussion and Implications
The study findings point to the role of the social network, even if indirect, in promoting self-protective behaviors among the oldest segment of society. Policymakers should collaborate with the social networks of older adults in order to promote the adoption of self-protective behaviors. Such intervention might help to reduce the threat of infection among the most vulnerable age group.
BNT162b2 mRNA COVID-19 Vaccine Effectiveness in the Prevention of SARS-CoV-2 Infection: A Preliminary Report
Authors: Bianchi Francesco Paolo , Germinario Cinzia Annatea , Migliore Giovanni , Vimercati Luigi , Martinelli Andrea , Lobifaro Annamaria , Tafuri Silvio , Stefanizzi Pasquale ,   Control Room Working Group, Amoruso Fabio , Capodiferro Luca , De Crescenzio Irene , Furio Alessandro , Lattanzio Sabrina , Nozza Mirella , Parnoffi Nicole , Rella Michela , Riformato Giacomo , Spinelli Giuseppe , Manicone Annalucia 
Published: May 2021

Abstract
In the preregistration trial, data on efficacy of BNT162b2 mRNA vaccine against SARS-CoV-2 infection were not collected. This study aimed to evaluate vaccine effectiveness (VE) against documented infection. Bari Policlinico University Hospital health care workers (HCWs) who completed the vaccination schedule were matched with HCWs who had refused vaccination. VE for documented infection was 61.9% (95% confidence interval [CI], 19.2%–82.0%) 14–20 days after first dose, 87.9% (95% CI, 51.7%–97.0%) 21–27 days after first dose, and 96.0% (95% CI, 82.2%–99.1%) 7 or more days after second dose. Unvaccinated HCWs remain a concern in the context of the pandemic emergency.
MALDI-TOF Mass Spectrometry and 16S rRNA Gene Sequence Analysis for the Identification of Foodborne Clostridium Spp
Authors: Sulaiman Irshad M , Miranda Nancy , Simpson Steven 
Published: May 2021

Abstract

Background
Clostridium is a genus of Gram-positive, spore-forming, anaerobic bacteria comprising approximately 100 species. Some Clostridium spp. (C. botulinum, C. perfringens, C. tetani, and C. difficile) have been recognized to cause acute food poisoning, botulism, tetanus, and diarrheal illness in humans. Thus, rapid identification of Clostridium spp. is critical for source-tracking of contaminated food and to understand the transmission dynamics of these foodborne pathogens.

Objective
This study was carried out to rapidly identify Clostridium-like isolates by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and rRNA sequencing methods.

Method
Thirty-three Clostridium-like isolates were recovered from various baby food and surveillance samples. Species identification of these isolates was accomplished using the VITEK MS system. Sequence characterization of the 16S rRNA region was done on an ABI 3500xL Genetic Analyzer.

Results
The VITEK MS system identified 28 of the 33 Clostridium-like isolates with a high confidence value (99.9%); no identification was observed for the remaining five isolates. Nucleotide sequencing of the 16S rRNA region identified all 33 Clostridium-like isolates. Furthermore, while characterizing the 16S rRNA gene, 11 distinct Clostridium spp. (Clostridium aciditolerans, Clostridium aerotolerans, Clostridium argentinense, Clostridium beijerinckii, Clostridium bifermentans, Clostridium butyricum, Clostridium cochlearium, Clostridium difficile, Clostridium perfringens, Clostridium sporogenes, and Clostridium subterminale) were recognized among the 33 Clostridium-like isolates. One of the Clostridium-like isolates was identified as Citrobacter amalonaticus by both diagnostic methods. The generated 16S rRNA sequences matched completely (100%) with sequences available in GenBank for Clostridium and Citrobacter species. Species identification attained using the VITEK MS for the Clostridium-like isolates was comparable to that from the 16S rRNA sequencing-based data.

Conclusions
The VITEK MS and 16S rRNA sequence analysis can be implemented in the species identification of Clostridium spp. isolates of public health importance.

Highlights
MALDI-TOF MS and 16S rRNA sequencing can be used in the species identification of Clostridium species.
In Vaccines We Trust? The Effects of the CIA’s Vaccine Ruse on Immunization in Pakistan
Authors: Martinez-Bravo Monica , Stegmann Andreas 
Published: May 2021

Abstract
In July 2011, the Pakistani public learnt that the Central Intelligence Agency (CIA) had used a vaccination campaign as cover to capture Osama Bin Laden. The Taliban leveraged on this information and launched an anti-vaccine propaganda campaign to discredit vaccines and vaccination workers. We evaluate the effects of these events on immunization by implementing a difference-in-differences strategy across cohorts and districts. We find that vaccination rates declined between 23% and 39% in districts in the 90th percentile of Islamist support relative to those in the 10th percentile. These results suggest that information discrediting vaccination campaigns can negatively affect trust in health services and demand for immunization.
Response to theJournal of ForestryArticle: “US Forest Service Implementation of the National Environmental Policy Act: Fast, Variable, Rarely Litigated, and Declining”
Authors: Morgan Todd A , Niccolucci Michael J , Berg Erik C 
Published: May 2021

AbstractThe Fleischman et al. (2020a) article on US Forest Service (FS) implementation of the National Environmental Policy Act (NEPA) correctly identified a decline in the number of NEPA analyses, but several conclusions were not supported by the data used. After analyzing their and other relevant data, we reached substantially different conclusions. Using FS budget data, we found budgets supporting NEPA analyses to be flat to increasing. Likewise, using FS accomplishment data, we found several FS land-management activities increased as others remained flat or decreased. The three types of NEPA analyses took statistically significant different times to complete, and time to complete analyses declined little over 15 years. We also found that litigation rates varied substantially by NEPA analysis type, resource purpose, and FS region. Conducting NEPA analyses is a necessary step in federal land management. However, resources invested in NEPA analyses represent an opportunity cost, and the success of the FS is better measured by on-the-ground accomplishments rather than number of NEPA analyses produced.
Local radiotherapy for pleural dissemination of thymic tumors after initial treatment
Authors: Okazaki Dai , Shibamoto Yuta , Yanagi Takeshi , Ishikura Satoshi , Kondo Takuhito , Yamada Yuki , Niwa Masanari 
Published: May 2021

Abstract
Pleural dissemination is a common pattern of failure after initial treatment of thymoma and thymic carcinoma, but there is no standardized treatment. As these tumors are relatively radiosensitive, we investigated the effectiveness of radiotherapy. Twenty patients underwent 33 series of local radiotherapy for 96 pleural dissemination lesions after initial treatment. Conventional radiotherapy (CRT), tomotherapy, and combination of the two were employed in 19, 13, and 1 series, respectively. The median follow-up period after the first irradiation for pleural dissemination was 46 months (range, 14–161). For all 20 patients, overall survival (OS) rates from initial radiotherapy for pleural dissemination were 100% at three years and 86% at five years. Progression-free survival (PFS) rates after 33 series of radiotherapy were 30% at three years and 16% at five years. Local control (LC) rates for 96 lesions were 98% at three years and 96% at five years. In-field recurrence was observed in only two among the 96 lesions. One patient (5%) developed grade 3 radiation pneumonitis and another (5%) developed grade 3 pericardial effusion. No other serious adverse events were observed. When disseminated nodules can be covered within localized fields, local radiotherapy may be a treatment option. Using tomotherapy, multiple lesions can be treated safely.
Prescription Antibiotic Use Among the US population 1999–2018: National Health and Nutrition Examination Surveys
Authors: Petersen Molly R , Cosgrove Sara E , Quinn Thomas C , Patel Eshan U , Kate Grabowski M , Tobian Aaron A R 
Published: May 2021

Abstract

Background
Antibiotic resistance has been identified as a public health threat both in the United States and globally. The United States published the National Strategy for Combating Antibiotic Resistance in 2014, which included goals to reduce inappropriate outpatient antibiotic use.

Methods
This cross-sectional study was conducted using National Health and Nutrition Examination Surveys (NHANES) years 1999–2018. Weighted prevalence of past 30-day nontopical outpatient antibiotic use was calculated, as well as the change in prevalence from 1999–2002 to 2015–2018 and 2007–2010 to 2015–2018, both overall and for subgroups. Associations with past 30-day nontopical outpatient antibiotic use in 2015–2018 were examined using predictive margins calculated by multivariable logistic regression.

Results
The overall prevalence of past 30-day nontopical outpatient antibiotic use adjusted for age, sex, race/ethnicity, poverty status, time of year of the interview, and insurance status from 1999–2002 to 2015–2018 changed significantly from 4.9% (95% CI, 3.9% to 5.0%) to 3.0% (95% CI, 2.6% to 3.0%), with the largest decrease among children age 0–1 years. From 2007–2010 to 2015–2018, there was no significant change (adjusted prevalence ratio [adjPR], 1.0; 95% CI, 0.8 to 1.2). Age was significantly associated with antibiotic use, with children age 0–1 years having significantly higher antibiotic use than all other age categories &gt;6 years. Being non-Hispanic Black was negatively associated with antibiotic use as compared with being non-Hispanic White (adjPR, 0.6; 95% CI, 0.4 to 0.8).

Conclusions
While there were declines in antibiotic use from 1999–2002 to 2015–2018, there were no observed declines during the last decade.
The Path to Surgical Robotics in Neurosurgery
Authors: Khanna Omaditya , Beasley Ryan , Franco Daniel , DiMaio Simon 
Published: May 2021

Abstract
Robotic systems may help efficiently execute complicated tasks that require a high degree of accuracy, and this, in large part, explains why robotics have garnered widespread use in a variety of neurosurgical applications, including intracranial biopsies, spinal instrumentation, and placement of intracranial leads. The use of robotics in neurosurgery confers many benefits, and inherent limitations, to both surgeons and their patients. In this narrative review, we provide a historical overview of robotics and its implementation across various surgical specialties, and discuss the various robotic systems that have been developed specifically for neurosurgical applications. We also discuss the relative advantages of robotic systems compared to traditional surgical techniques, particularly as it pertains to integration of image guidance with the ability of the robotic arm to reliably execute pre-planned tasks. As more neurosurgeons adopt the use of robotics in their practice, we postulate that further technological advancements will become available that will help achieve improved technical capabilities, user experience, and overall patient clinical outcomes.
Evidence of Bilateral Localized, but Not Widespread, Pressure Pain Hypersensitivity in Patients With Upper Extremity Tendinopathy/Overuse Injury: A Systematic Review and Meta-Analysis
Authors: Fernández-de-las-Peñas César , Navarro-Santana Marcos J , Cleland Joshua A , Arias-Buría José L , Plaza-Manzano Gustavo 
Published: May 2021

Abstract

Objective
The presence of altered nociceptive pain processing in patients with upper extremity tendinopathy/overuse injury is conflicting. Our aim was to compare pressure pain thresholds (PPTs) in symptomatic and distant pain-free areas between patients with upper extremity tendinopathy/overuse injury and controls.

Methods
Five databases were searched from inception to October 15, 2020. The authors selected case-control studies comparing PPTs between individuals with upper extremity tendinopathy/overuse injury and pain-free controls. Data were extracted for population, diagnosis, sample size, outcome, and type of algometer. Results were extracted by 3 reviewers. The methodological quality/risk of bias (Newcastle-Ottawa Quality Assessment Scale) and evidence level (Grading of Recommendations Assessment, Development and Evaluation approach) were assessed. Meta-analyses of symptomatic, segment-related, and distant pain-free areas were compared.

Results
The search identified 807 publications with 19 studies (6 shoulder, 13 elbow) eligible for inclusion. The methodological quality ranged from fair (48%) to good (37%). Patients exhibited lower bilateral PPTs than controls at the symptomatic area (affected side: MD = −175.89 kPa [95% CI = −220.30 to −131.48 kPa]; nonaffected side: MD = −104.50 kPa [95% CI = −142.72 to −66.28 kPa]) and the segment-related area (affected side: MD = −150.63 kPa [95% CI = −212.05 to −89.21 kPa]; nonaffected side: MD = −170.34 kPa [95% CI = − 248.43 to −92.25]) than controls. No significant differences in PPTs over distant pain-free areas were observed.

Conclusion
Low to moderate quality evidence suggests bilateral hypersensitivity to pressure pain at the symptomatic and contralateral/mirror areas in patients with upper extremity tendinopathies/overuse injury. Moderate quality of evidence supports bilateral pressure pain sensitivity in the segment-related area (neck) in lateral epicondylalgia, but not in subacromial impingement syndrome. No evidence of widespread pressure pain hyperalgesia was reported.

Impact
Early identification of people with altered pain modulation could guide clinicians in treatment strategies. This review shows that there is a complex interplay between peripheral and central pain mechanisms in upper extremity tendinopathies/overuse injuries and that there likely are different subgroups of patients with upper extremity conditions.
Spatial Reconstruction of the Early Hepatic Transcriptomic Landscape After an Acetaminophen Overdose Using Single-Cell RNA-Sequencing
Authors: Umbaugh David S , Ramachandran Anup , Jaeschke Hartmut 
Published: May 2021

Abstract
An acetaminophen (APAP) overdose is the most common cause of acute liver failure in the United States. A hallmark characteristic of APAP hepatotoxicity is centrilobular necrosis. General, innate mechanisms such as lower amounts of GSH and higher cytochrome P450 2e1 expression in pericentral (PC) hepatocytes are known to contribute to the differences in susceptibility to cell injury between periportal (PP) hepatocytes and PC hepatocytes. Although a sequence of molecular events involving formation of the reactive metabolite N-acetyl-p-benzoquinone imine, GSH depletion, oxidative stress, and c-Jun N-terminal kinase activation define the early cell stress trajectory following APAP exposure, their activation in PC versus PP hepatocytes is not well characterized. By using single-cell RNA-sequencing, we provide the first reconstruction of the early transcriptomic APAP liver lobule after validation of our methodology using human liver single-cell RNA-sequencing data. Two hours after APAP treatment, we find that PP hepatocytes progress along the APAP stress axis to oxidative stress, before resolving injury due to innate and adaptive mechanisms. However, PC hepatocytes continue along this stress axis as indicated by activation of mitogen-activated protein kinase genes, which is absent in PP hepatocytes. We also identify a population of glutamine synthetase enriched PC hepatocytes in close proximity to the central vein, where a stepwise induction of a stress program culminated in cell death. Collectively, these findings elucidate a molecular sequence of events distinguishing the differential response to APAP exposure between PP and PC hepatocytes and identify a subset of uniquely susceptible PC hepatocytes.
Genomic and Hormonal Biomarkers of Phthalate-Induced Male Rat Reproductive Developmental Toxicity Part II: A Targeted RT-qPCR Array Approach That Defines a Unique Adverse Outcome Pathway
Authors: Gray Leon Earl , Lambright Christy S , Conley Justin M , Evans Nicola , Furr Johnathan R , Hannas Bethany R , Wilson Vickie S , Sampson Hunter , Foster Paul M D 
Published: May 2021

Abstract
Previously, we demonstrated that exposure to some diortho-phthalate esters during sexual differentiation disrupts male reproductive development by reducing fetal rat testis testosterone production (T Prod) and gene expression in a dose-related manner. The objectives of the current project were to expand the number of test compounds that might reduce fetal T Prod, including phthalates, phthalate alternatives, pesticides, and drugs, and to compare reductions in T Prod with altered testis mRNA expression. We found that PEs that disrupt T Prod also reduced expression of a unique “cluster” of mRNAs for about 35 genes related to sterol transport, testosterone and insulin-like hormone 3 hormone syntheses, and lipoprotein signaling and cholesterol synthesis. However, phthalates had little or no effect on mRNA expression of genes in peroxisome proliferator-activated receptor (PPAR) pathways in the fetal liver, whereas the 3 PPAR agonists induced the expression of mRNA for multiple fetal liver PPAR pathway genes without reducing testis T Prod. In summary, phthalates that disrupt T Prod act via a novel adverse outcome pathway including down regulation of mRNA for genes involved in fetal endocrine function and cholesterol synthesis and metabolism. This profile was not displayed by PEs that did not reduce T Prod, PPAR agonists or the other chemicals. Reductions in fetal testis gene expression and T Prod in utero can be used to establish relative potency factors that can be used quantitatively to predict the doses of individual PEs and mixtures of phthalates that produce adverse reproductive tract effects in male offspring.
Measure for Measure: Comparing Survey Based Estimates of Income and Consumption for Rural Households
Authors: Carletto Gero , Tiberti Marco , Zezza Alberto 
Published: May 2021

AbstractThis paper uses a large database of surveys of household incomes to characterize income underreporting in household surveys in low- and middle-income countries. The objective is to document (a) the extent of this underreporting, and (b) whether and how it varies systematically with respondent, household, income, and survey design features. Drawing on rural household data from 20 developing and transition countries, and using consumption expenditure as a benchmark, results indicate that the observed income/consumption ratios are very small, being on average around 0.76. Results suggest that income underreporting is systematically associated with household and survey characteristics. In particular, the degree of underreporting is strongly associated with the income source, with agricultural income being the component suffering more than any other components from underreporting. The analysis also provides evidence supporting the well-established proposition that underreporting tends to increase with household welfare: richer households appear to underreport income more. Implications for survey design and for future research are drawn.