bluuebunny/crossref_metadata_2025_split huggingface dataset:
Open-Access Academic Articles Requires Peer-Review Makeover: Consistency Is the Key
Authors: Ostafiński Witold 
Published: November 2021

The internet has greatly altered the way that people and institutions communicate. One of the most recent changes is the growth of the Open Access (OA) model, where research articles are provided free of charge to readers online. Such changes are having a domino effect on traditional communication. In most cases, articles in professional journals have been evaluated by a strict peer-review system. However, due to the inherent problems with these peer reviews, such as the length of time it takes referees to complete their analysis and the reviewer’s personal biases and potential unethical behavior, there is a growing consensus that a different review method needs to be developed for OA articles. In addition, the internet has led to the development of many new professional journals, which range from poor to excellent, based on the articles accepted. Unfortunately, some journals are being published solely for monetary gain from high author fees.Presently, different review methods are being suggested or implemented for articles, such as ranking systems, online commentaries and crowdsourcing, Also, various institutions are publishing lists that rate academic journals on their quality level. Such experimentation of review models is important. However, after the trial period, the primary OA sources need to agree on using the same review model. Consistency of evaluation is critical for readers to be able to make objective comparisons of scholastic articles from one OA site to another.
Detrimental Effect of Ozone on Pathogenic Bacteria
Authors: Rangel Karyne , Cabral Felipe O , Lechuga Guilherme Curty , Carvalho João P.R.S. , Villas-Bôas Maria H.S. , Midlej Victor , De-Simone Salvatore Giovanni 
Published: November 2021

(1) Background: Disinfection of medical devices designed for clinical use associated or not with the growing area of tissue engineering is an urgent need. However, traditional disinfection methods are not always suitable for some biomaterials, especially those sensitive to chemical, thermal, or radiation. Therefore, the objective of this study was to evaluate the minimal concentration of ozone gas (O3) necessary to control and kill a set of sensitive or multi-resistant Gram-positive and Gram-negative bacteria. The cell viability, membrane permeability, and the levels of reactive intracellular oxygen (ROS) species were also investigated; (2) Material and Methods: Four standard strains and a clinical MDR strain were exposed to low doses of ozone at different concentrations and times. Bacterial inactivation (cultivability, membrane damage) was investigated using colony counts, resazurin as a metabolic indicator, and propidium iodide (PI). A fluorescent probe (H2DCFDA) was used for the ROS analyses; (3) Results: No reduction in the count colony was detected after O3 exposure, compared to the control group. However, the cell viability of E. coli (30%), P. aeruginosa (25%), and A. baumannii (15%) was reduced considerably. The bacterial membrane of all strains was not affected by O3 but presented a significant increase of ROS in E. coli (90 ± 14%), P. aeruginosa (62.5 ± 19%), and A. baumanni (52.6 ± 5%); (4) Conclusion: Low doses of ozone were able to interfere in the cell viability of most strains studied, and although it does not cause damage to the bacterial membrane, increased levels of reactive ROS are responsible for causing a detrimental effect in the lipids, proteins, and DNA metabolism.
Special Issue on Advanced Precision Engineering for Digital Transformation
Authors: Yamamura Kazuya , Shirase Keiichi 
Published: November 2021

“The digital transformation can be understood as the changes that the digital technology causes or influences in all aspects of human life” (definition by Prof. Erik Stolterman). In order to manufacture high value-added products and create a sustainable society, the digital transformation, based on advanced precision engineering, will be an urgent task within manufacturing systems.
This special issue consists of eight excellent research papers that focus on advanced precision engineering in manufacturing systems. All research papers were presented at the 18th International Conference on Precision Engineering (ICPE2020). The ICPE2020 covered various topics, including digital design, CAD/CAM technology, traditional cutting/grinding, non-traditional additive manufacturing, machine tools, measurement, robotics, control, and others. Held during the COVID-19 pandemic, it was a virtual conference that saw 189 papers presented in the oral session and 38 papers presented in poster session. The COVID-19 pandemic demands the innovation of current manufacturing systems with new concepts and methodologies, and the editors hope that the research papers in this special issue give us valuable information for the digital transformation of manufacturing systems.
All papers were refereed through careful peer reviews. The editors deeply appreciate the efforts and excellent work of all the authors and anonymous reviewers in realizing this special issue. Finally, we hope that future research on precision engineering in manufacturing will further contribute to the achievement of the Sustainable Development Goals (SDGs) of our global society.
99mTc labeled genistein kits: development methods and quality control for breast cancer radiotracer applications
Authors: Ramdhani Danni , Sriy Maula Eka , W Eva Maria , Listiani Nita , Mustarichie Resmi , Watabe Hiroshi 
Published: November 2021

Abstract
Objective.

Selective estrogen receptor modulators (SERMs) have been widely used to treat breast cancer, osteoporosis, and postmenopausal symptoms. SERMs have an affinity for estrogen receptors (ER) in target tissues and resist stimulation of the breast, bone, and endometrium. Genistein as an isoflavone compound that has a high affinity for ERβ targets makes it a potential target or prognostic marker for breast cancer. This study was carried out to develop 99mTc-genistein kit that can be used to detect breast cancer.
Methods.

The synthesis process and quality control were investigated to obtain the optimal formula for the ratio of a substance, reducing agent, optimal conditions of the synthesis reaction, physicochemical properties of the kit, and its stability to meet the requirements of radiochemical purity.
Results.

The radiochemical purity in the development of the radiopharmaceutical kit was 93.25% ± 0.30%. The physicochemical properties of the kit preparations showed hydrophilic properties, good plasma protein binding, no electrical charge, and were stable at storage temperatures.
Conclusions.

The radiochemical purity of the radiopharmaceutical kits meets the requirements of the United State Pharmacopeia and has good physicochemical properties to be developed into kits.
Brucein D augments the chemosensitivity of gemcitabine in pancreatic cancer via inhibiting the Nrf2 pathway
Authors: Zhang Juan , Xu Hong-Xi , Cho William Chi Shing , Cheuk Wah , Li Yang , Huang Qiong-Hui , Yang Wen , Xian Yan-fang , Lin Zhi-Xiu 
Published: November 2021

Abstract
Background
Gemcitabine (GEM) is a chemotherapeutic drug used to treat pancreatic ductal adenocarcinoma carcinoma (PDAC), but chemoresistance is often encountered clinically. Nrf2, an oxidative stress responsive transcription factor, is an important contributor to chemoresistance and is closely associated with poor prognosis of PDAC. Brucein D (BD), a natural compound has been reported to exert anti-tumor effect in several cancers including PDAC. In this study, we aimed to investigate the efficacy of the BD and Nrf2 axes on the chemosensitivity of GEM and attempted to elucidate its underlying mechanism.
Methods
Clinical study and GEPIA database analysis was first conducted to identify the expression of Nrf2 in PDAC. We then established cell lines with stable deletion of Nrf2 through lentivirus transfecting into PDAC cells. Quantitative real-time PCR (qRT-PCR) and Western blotting were performed to measure the expression of Nrf2. The effects of BD and Nrf2 axes on PDAC cell proliferation, colony-formation, tumor growth and chemosensitivity were determined both in vitro and in vivo. Orthotopic xenograft and genetically engineered KPC mouse models of PDAC were used to evaluate the anti-cancer effects of BD and GEM.
Results
Nrf2 was highly expressed in PDAC from clinical study and GEPIA analysis. Gain-and lost-function study demonstrated that Nrf2 aggravated the chemosensitivity of GEM of PDAC cells both in vitro and in vivo. We further found that BD efficiently inhibited PDAC cells proliferation and enhanced the chemosensitivity of GEM. Mechanistic studies revealed that BD sensitized the GEM in PDAC cells through the ubiquitin-proteasome-dependent degradation of Nrf2, and then downregulate the Nrf2 pathway. Silencing of Nrf2 plus BD resulted in a better inhibitory effect of GEM. In contrast, Nrf2 activation aggravated the chemosensitivity of GEM, indicating that the action of BD was Nrf2 dependent. Furthermore, the efficacy of BD alone and in combination with GEM was validated on both orthotopic xenograft and genetically engineered KPC mouse models.
Conclusions
BD was proven to enhance the chemosensitivity of GEM in PDAC through inhibition of the Nrf2 pathway. Our experimental findings indicate that BD, a potent Nrf2 inhibitor, holds promise for further development into a novel adjuvant therapy for PDAC.
Detrimental Health Relationship Between Blood Cadmium and the Red Blood Cell Folate Level
Authors: Wang Bo-kai , Chen Wei-Liang 
Published: November 2021

Abstract
Background and Aims: Increasing studies have demonstrated the association between heavy metal pollution and micronutrients, especially folate. However, the relationship between cadmium and folate remains rarely discussed. In this study, we aim to explore the potential correlation between cadmium and folate in human population, and highlight the possible mechanism of cadmium impacting on human health.Method and Results: We utilized the National Health and Nutrition Examination Survey(NHANES) 2017-2018 data with 5690 participants in this study. Multivariable linear regression models were adopted to investigate the serum cadmium levels and RBC folate concentration. Gender differences in this correlation was also explored. A significant reverse relationship was found between serum cad-mium and RBC folate, either in male or female.Conclusions: A negative relationship between serum cadmium levels and levels of RBC folate either in male or female group in the U.S adult population was found in this study. Nevertheless, due to the general limitations of the NHANES data, as a cross-sectional study, further prospective investigation is needed to discover the causality of cadmium in folate status, and to determine whether or not the folate supplement have a beneficial influence against heavy metal toxicities.
Inhibition of Immunoglobulin E Attenuates Pulmonary Hypertension
Authors: Ting Shu , Ying Liu , Yitian Zhou , Zhou Zhou , Peiran Yang , Jinqiu Li , Yanjiang Xing , Xiaomin Song , Bolun Li , Junling Pang , Xin Ning , Xianmei Qi , Changming Xiong , Hang Yang , Qianlong Chen , Jingyu Chen , Ying Yu , Jing Wang , Chen Wang 
Published: November 2021

Abstract
Pulmonary hypertension (PH) is a severe cardiopulmonary disease characterized by pathological vascular remodeling in the lung. Immunoglobulin E (IgE) is known to participate in aortic vascular remodeling, but whether IgE mediates pulmonary vascular remodeling in PH is unknown. Here, we found serum IgE elevation in PAH patients, hypoxia-induced PH mice and monocrotaline (MCT)-induced PH rats. Combining animal model of PH with single-cell RNA sequencing, we found IgE production in the lung tissues of PH mice. Neutralizing IgE with an anti-IgE antibody was effective in preventing PH development in mice and rat models. The IgE receptor FcεRIα was also upregulated in PH lung tissues and Fcer1a deficiency prevented the development of PH in mice. Single-cell RNA-seq revealed that FcεRIα was mostly expressed in mast cells, and mast cell-specific Fcer1a knockout protected against PH in mice. Further mechanistic experiments revealed that IgE-activated mast cells produced interleukins IL6 and IL13, which subsequently promoted vascular muscularization. Clinically approved IgE antibody Omalizumab alleviated the progression of established PH in rats. Using genetic and pharmacological approaches, we have demonstrated that blocking IgE- FcεRIα signaling may hold potential for the treatment of PAH.
The Impact of Meaning in Life and Reasons for Living on Suicidal Behaviour in Young Adults: A Systematic Review
Authors: Flynn Evan 
Published: November 2021

Abstract
A systematic review is presented which aims to synthesise relevant literature focused on the influence of Meaning in Life (MiL) and Reasons for Living (RFL) on Suicidal Behaviour (SB) in young adults. Twelve papers of suitable quality were sourced through a rigorous search strategy and a narrative synthesis was performed. This revealed that RFL and MiL act as protective factors against SB in young adults. Additional findings revealed a cultural universality of the protective aspect of MiL/RFL on SB, as well as further insights into the sub-components of life meaning- Search for Meaning in Life and Presence of Meaning in Life. The associated risk factors of SB- Hopelessness, Life Regard and Mental Pain, are also discussed in relation to MiL and RFL. This study paves the way for further research on the same topic and draws attention to something that is frequently neglected in the psychiatric community- consideration of the transcendental aspects of human nature.
Workforce problems at rural public health-centres in India: A WISN retrospective analysis and national-level modelling study
Authors: Nair Aatmika , Jawale Yash , Dubey Sweta R , Dharmadhikari Surabhi , Zadey Siddhesh 
Published: November 2021

Abstract
BACKGROUND
Rural India has a severe shortage of human resources for health (HRH). The National Rural Health Mission (NRHM) deploys HRH in the rural public health system to tackle shortages. Sanctioning under NRHM does not account for workload resulting in inadequate and inequitable HRH allocation. The Workforce Indicators of Staffing Needs (WISN) approach can identify shortages and inform appropriate sanctioning norms. India currently lacks nationally-relevant WISN estimates. We used existing data and modelling techniques to synthesize such estimates.
METHODS
We conducted a retrospective analysis of existing survey data for 93 facilities from 5 states over 8 years to create WISN calculations for HRH cadres at primary and community health centres (PHCs and CHCs) in rural areas. We modelled nationally-representative average WISN-based requirements for specialist doctors at CHCs, general doctors and nurses at PHCs and CHCs. For 2019, we calculated national and state-level overall and per-centre WISN differences and ratios to depict shortage and workload pressure. We checked correlations between WISN ratios for cadres at a given centre-type to assess joint workload pressure. We evaluated the gaps between WISN-based requirements and sanctioned posts to investigate suboptimal sanctioning through concordance analysis and difference comparisons.
RESULTS
In 2019, at the national-level, WISN differences depicted workforce shortages for all considered HRH cadres. WISN ratios showed that nurses at PHCs and CHCs, and all specialist doctors at CHCs had very high workload pressure. States with more workload on PHC-doctors also had more workload on PHC-nurses depicting an augmenting or compounding effect on workload pressure across cadres. A similar result was seen for CHC-specialist pairs - physicians and surgeons, physicians and paediatricians, and paediatricians and obstetricians-gynaecologists. We found poor concordance between current sanctioning norms and WISN-based requirements with all cadres facing under-sanctioning. We also present across-state variations in workforce problems, workload pressure and sanctioning problems.
CONCLUSION
We demonstrate the use of WISN calculations based on available data and modelling techniques for national-level estimation. Our findings suggest prioritising nurses and specialists in the rural public health system and updating the existing sanctioning norms based on workload assessments. Workload-based rural HRH deployment can ensure adequate availability and optimal distribution.
Early Disruption of Synaptic Function, Impairment of Plasticity, and Decreased of Cortical Circuit Connectivity in an Alzheimer’S Mouse Model of Amyloid Deposition
Authors: Chen Chang , Wei Jing , Ma Xiaokuang , Zhang Le , Nehme Antoine , Cui Yuehua , Ferguson Deveroux , Qiu Shenfeng , Bai Feng 
Published: November 2021

Abstract
Mutations in genes encoding amyloid precursor proteins and presenilins lead to increased β-amyloid (Aβ) production and cause familial Alzheimer’s disease (AD), a neurodegenerative disorder often associates with aging and features synapse loss and impaired synaptic plasticity. Aβ deposition is a pathological hallmark of AD. It is currently unknown whether and how AD risk alleles affects development of brain circuit function, and whether subtle synaptic pathology occurs prior to overt Aβ deposition. Transgenic mutated APP/PS1 over-expression mice lines are key tools to study molecular mechanisms of AD pathogenesis. Among these mice lines, the 5XFAD mice rapidly recapitulate key features of AD pathology, and have proven utility in studying amyloid plaque formation and Aβ-induced neurodegeneration. We reason that transgenic mutant APP/PS1 over-expression may lead to neurodevelopmental defects in early cortical neurons as a result of continuous APP/Aβ expression from early life. We first explored age-dependent plasticity changes in prefrontal cortical circuits in the 5XFAD mice, and found that at an early age (6-8 wks old) that does not produce overt impairment of hippocampus LTP, layer (L) 5 neuron circuit plasticity, measured by long term potentiation (LTP), is impaired. In addition, L5 neurons, which are reportedly vulnerable cortical neuron populations, show reduced mEPSC amplitude and frequency in early postweaning ages, indicating impaired synaptic transmission as a result of transgenic APP/Aβ overloading during early postnatal development. These functional changes were corroborated by the morphological findings of smaller, sparser dendritic spines on L5 pyramidal neurons, indicative of impaired prefrontal circuit function. Lastly, laser scanning photostimulation (LSPS) combined with glutamate uncaging revealed that L2/3 > L5 cortical connectivity was decreased at this early age. Our results suggest 5XFAD transgenic mutant APP/PS1 over expression causes developmental defects of cortical circuits, which could contribute to the age-dependent synaptic pathology and neurodegeneration later in life.
In Vitro Antitumor Evaluation of Acrylic Acid Derivatives Bearing Quinolinone Moiety as Novel Anticancer Agents
Authors: Zaki Islam , Eid Sarah A. , Elghareb Mohamed S. , Abas Al-Shimaa M. , Mersal Gaber , Mohammed Faten Z. 
Published: November 2021

Background:
Due to the emergence of resistance to available anticancer agents, the demand for new cytotoxic
agents has grown.

Objective:
This study aims at synthesis and cytotoxic evaluation of new acrylic acid derivatives bearing quinolinone
and halogenated quinolinone derivatives against three cancer cell lines.

Methods:
New acrylic acid derivatives bearing quinolinone and halogenated quinolinone moieties were synthesized
and screened for their cytotoxic activity against breast MCF-7, liver HepG2, and colon HCT-116 cancer cell lines.

Results:
Molecules 3 and 8 showed the most potent cytotoxic activity against HCT-116. DNA flow cytometry assay
showed cell cycle arrest at the G1 phase and cellular apoptosis. Moreover, molecules 3 and 8 showed cyclin-dependent
kinase 2 (CDK2) inhibitory activity compared to the untreated control sample.

Conclusion:
Acrylic acid derivatives bearing quinolinone and halogenated quinolinone moieties represent an important
core and could be used as a lead for further development of drug compounds in order to achieve promising therapeutic
results.
Object detection and used car price predicting analysis system (UCPAS) using machine learning technique
Authors: Yadav Anu , Kumar Ela , Yadav Piyush Kumar 
Published: November 2021

The highly interesting research area that noticed in the last few years is object detection and find out the prediction based on the features that can be benefited to consumers and the industry. In this paper, we understand the concept of object detection like the car detection, to look into the price of a second-hand car using automatic machine learning methods. We also understand the concept of object detection categories. Nowadays, the most challenging task is to determine what is the listed price of a used car on the market, Possibility of various factors that can drive a used car price. The main objective of this paper is to develop machine learning models which make it possible to accurately predict the price of a second-hand car according to its parameter or characteristics. In this paper, implementation techniques and evaluation methods are used on a Car dataset consisting of the selling prices of various models of car across different cities of India. The outcome of this experiment shows that clustering with linear regression and Random Forest model yield the best accuracy outcome. The machine learning model produces a satisfactory result within a short duration of time compared to the aforementioned self.