bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magneto-acoustic oscillations observed in a solar plage region
Authors: Ji Haisheng , Hashim Parida , Hong Zhenxiang , Xu Zhe , Shen Jinhua , Ji Kaifan , Cao Wenda 
Published: August 2021

Abstract
We gave an extensive study for the quasi-periodic perturbations on the time profiles of the line of sight (LOS) magnetic field in 10 × 10 sub-areas in a solar plage region (corresponds to a facula on the photosphere). The perturbations are found to be associated with the enhancement of He I 10830 Å absorption in a moss region, which is connected to loops with million-degree plasma. FFT analysis to the perturbations gives a kind of spectrum similar to that of Doppler velocity: a number of discrete periods around 5 minutes. The amplitudes of the magnetic perturbations are found to be proportional to magnetic field strength over these sub-areas. In addition, magnetic perturbations lag behind a quarter of the cycle in the phase with respect to the p-mode Doppler velocity. We show that the relationships can be well explained with an MHD solution for the magneto-acoustic oscillations in high-β plasma. Observational analysis also shows that, for the two regions with the stronger and weaker magnetic field, the perturbations are always anti-phased. All findings show that the magnetic perturbations are actually magneto-acoustic oscillations on the solar surface, the photosphere, powered by p-mode oscillations. The findings may provide a new diagnostic tool for exploring the relationship between magneto-acoustic oscillations and the heating of the solar upper atmosphere, as well as their role in helioseismology.
A correction method for the telluric absorptions and application to Lijiang Observatory
Authors: Lu Kai-Xing , Zhang Zhi-Xiang , Huang Ying-Ke , Ren An-Bing , Xu Liang , Feng Hai-Cheng , Xin Yu-Xin , Ding Xu , Yu Xiao-Guang , Bai Jin-Ming 
Published: August 2021

Abstract
Observing a telluric standard star for correcting the telluric absorption lines of spectrum will take a significant amount of precious telescope time, especially in the long-term spectral monitoring project. Beyond that, it is difficult to select a suitable telluric standard star near in both time and airmass to the scientific object. In this paper, we present a method of correcting the telluric absorption lines by combining the advantages of long slit spectroscopy. By rotating the slit, we observed the scientific object and a nearby comparison star in one exposure, so that the spectra of both objects should have the same telluric transmission spectrum. The telluric transmission spectrum was constructed by dividing the observed spectrum of the comparison star by its stellar template, and was used to correct the telluric absorption lines of the scientific object. Using the long slit spectrograph of the Lijiang 2.4-meter telescope, we designed a long-term spectroscopic observation strategy, and finished a four-year spectroscopic monitoring for a pair of objects (an active galactic nucleus and a non-varying comparison star). We applied this method to correct the telluric absorption lines of the long-term monitored spectra by the Lijiang 2.4-meter telescope, and investigated the variation of the telluric absorptions at Lijiang Observatory. We found that the telluric absorption transparency is mainly modulated by the seasonal variability of the relative humidity, airmass and seeing. Using the scatter of the [OIII] λ5007 fluxes emitted from the narrow-line region of active galactic nuclei as an indicator, we found that the correction accuracy of the telluric absorption lines is 1%.
ProteinEvolverABC: coestimation of recombination and substitution rates in protein sequences by approximate Bayesian computation
Authors: Arenas Miguel 
Published: August 2021

Abstract

Motivation
The evolutionary processes of mutation and recombination, upon which selection operates, are fundamental to understand the observed molecular diversity. Unlike nucleotide sequences, the estimation of the recombination rate in protein sequences has been little explored, neither implemented in evolutionary frameworks, despite protein sequencing methods are largely used.

Results
In order to accommodate this need, here I present a computational framework, called ProteinEvolverABC, to jointly estimate recombination and substitution rates from alignments of protein sequences. The framework implements the approximate Bayesian computation approach, with and without regression adjustments and includes a variety of substitution models of protein evolution, demographics and longitudinal sampling. It also implements several nuisance parameters such as heterogeneous amino acid frequencies and rate of change among sites and, proportion of invariable sites. The framework produces accurate coestimation of recombination and substitution rates under diverse evolutionary scenarios. As illustrative examples of usage, I applied it to several viral protein families, including coronaviruses, showing heterogeneous substitution and recombination rates.

Availability and implementation
ProteinEvolverABC is freely available from https://github.com/miguelarenas/proteinevolverabc, includes a graphical user interface for helping the specification of the input settings, extensive documentation and ready-to-use examples. Conveniently, the simulations can run in parallel on multicore machines.

Supplementary information
Supplementary data are available at Bioinformatics online.
Increased H2O2 levels and p53 stabilization lead to mitochondrial dysfunction in XPC-deficient cells
Authors: Freire T S , Mori M P , Miranda J N F A , Muta L Y M , Machado F T , Moreno N C , Souza-Pinto N C 
Published: August 2021

Abstract
XPC deficiency is associated with mitochondrial dysfunction, increased mitochondrial H2O2 production and sensitivity to the Complex III inhibitor antimycin A (AA), through a yet unclear mechanism. We found an imbalanced expression of several proteins that participate in important mitochondrial function and increased expression and phosphorylation of the tumor suppressor p53 in Xeroderma pigmentosum complementation group C (XP-C) (XPC-null) cells compared with an isogenic line corrected in locus with wild-type XPC (XPC-wt). Interestingly, inhibition of p53 nuclear import reversed the overexpression of mitochondrial proteins, whereas AA treatment increased p53 expression more strongly in the XP-C cells. However, inhibition of p53 substantially increased XP-C cellular sensitivity to AA treatment, suggesting that p53 is a critical factor mediating the cellular response to mitochondrial stress. On the other hand, treatment with the antioxidant N-acetylcysteine increased glutathione concentration and decreased basal H2O2 production, p53 levels and sensitivity to AA treatment in the XPC-null back to the levels found in XPC-wt cells. Thus, the results suggest a critical role for mitochondrially generated H2O2 in the regulation of p53 expression, which in turn modulates XP-C sensitivity to agents that cause mitochondrial stress.
Recognition Memory is Associated with Distinct Patterns of Regional Gray Matter Volumes in Young and Aged Monkeys
Authors: Cooper C’iana P , Shafer Andrea T , Armstrong Nicole M , Rossi Sharyn L , Young Jennifer , Herold Christa , Gu Hong , Yang Yihong , Stein Elliot A , Resnick Susan M , Rapp Peter R 
Published: August 2021

Abstract
Cognitive aging varies tremendously across individuals and is often accompanied by regionally specific reductions in gray matter (GM) volume, even in the absence of disease. Rhesus monkeys provide a primate model unconfounded by advanced neurodegenerative disease, and the current study used a recognition memory test (delayed non-matching to sample; DNMS) in conjunction with structural imaging and voxel-based morphometry (VBM) to characterize age-related differences in GM volume and brain-behavior relationships. Consistent with expectations from a long history of neuropsychological research, DNMS performance in young animals prominently correlated with the volume of multiple structures in the medial temporal lobe memory system. Less anticipated correlations were also observed in the cingulate and cerebellum. In aged monkeys, significant volumetric correlations with DNMS performance were largely restricted to the prefrontal cortex and striatum. Importantly, interaction effects in an omnibus analysis directly confirmed that the associations between volume and task performance in the MTL and prefrontal cortex are age-dependent. These results demonstrate that the regional distribution of GM volumes coupled with DNMS performance changes across the lifespan, consistent with the perspective that the aged primate brain retains a substantial capacity for structural reorganization.
Standardized measurement of coronary inflammation using cardiovascular computed tomography: integration in clinical care as a prognostic medical device
Authors: Oikonomou Evangelos K , Antonopoulos Alexios S , Schottlander David , Marwan Mohammad , Mathers Chris , Tomlins Pete , Siddique Muhammad , Klüner Laura V , Shirodaria Cheerag , Mavrogiannis Michail C , Thomas Sheena , Fava Agostina , Deanfield John , Channon Keith M , Neubauer Stefan , Desai Milind Y , Achenbach Stephan , Antoniades Charalambos 
Published: August 2021

AbstractAimsCoronary computed tomography angiography (CCTA) is a first-line modality in the investigation of suspected coronary artery disease (CAD). Mapping of perivascular fat attenuation index (FAI) on routine CCTA enables the non-invasive detection of coronary artery inflammation by quantifying spatial changes in perivascular fat composition. We now report the performance of a new medical device, CaRi-Heart®, which integrates standardized FAI mapping together with clinical risk factors and plaque metrics to provide individualized cardiovascular risk prediction.Methods and resultsThe study included 3912 consecutive patients undergoing CCTA as part of clinical care in the USA (n = 2040) and Europe (n = 1872). These cohorts were used to generate age-specific nomograms and percentile curves as reference maps for the standardized interpretation of FAI. The first output of CaRi-Heart® is the FAI-Score of each coronary artery, which provides a measure of coronary inflammation adjusted for technical, biological, and anatomical characteristics. FAI-Score is then incorporated into a risk prediction algorithm together with clinical risk factors and CCTA-derived coronary plaque metrics to generate the CaRi-Heart® Risk that predicts the likelihood of a fatal cardiac event at 8 years. CaRi-Heart® Risk was trained in the US population and its performance was validated externally in the European population. It improved risk discrimination over a clinical risk factor-based model [Δ(C-statistic) of 0.085, P = 0.01 in the US Cohort and 0.149, P &lt; 0.001 in the European cohort] and had a consistent net clinical benefit on decision curve analysis above a baseline traditional risk factor-based model across the spectrum of cardiac risk.ConclusionMapping of perivascular FAI on CCTA enables the non-invasive detection of coronary artery inflammation by quantifying spatial changes in perivascular fat composition. We now report the performance of a new medical device, CaRi-Heart®, which allows standardized measurement of coronary inflammation by calculating the FAI-Score of each coronary artery. The CaRi-Heart® device provides a reliable prediction of the patient's absolute risk for a fatal cardiac event by incorporating traditional cardiovascular risk factors along with comprehensive CCTA coronary plaque and perivascular adipose tissue phenotyping. This integration advances the prognostic utility of CCTA for individual patients and paves the way for its use as a dual diagnostic and prognostic tool among patients referred for CCTA.
Coherence for nutrition: insights from nutrition-relevant policies and programmes in Burkina Faso and Nigeria
Authors: Billings Lucy , Pradeilles Rebecca , Gillespie Stuart , Vanderkooy Anna , Diatta Dieynab , Toure Mariama , Diatta Ampa Dogui , Verstraeten Roos 
Published: August 2021

Abstract
There is consensus that policy coherence is necessary for implementing effective and sustainable approaches to tackle malnutrition. We look at whether policies and programmes provide a coherent pathway to address nutrition priorities and if programmes are designed to deliver interventions aligned to the nutrition policy agenda in Nigeria and Burkina Faso. A systematic desk review was performed on nutrition-relevant policy and programme documents, obtained through grey literature searches and expert recommendations. We developed a framework with an impact pathway structure that includes five process steps, which was used to guide coding, data reduction and synthesis and structure the analysis. We assessed internal coherence along process steps within a given document and external coherence across process steps for explicitly linked policy/programme pairs. The majority of policies and programmes had partial internal coherence for both countries. The identification of relevant nutrition interventions to address challenges and reach objectives was the strongest connection within policies (16 out of 45 had complete coherence), while among programmes, the strongest connection was coverage indicators that measure interventions (9 out of 21 had complete coherence). Eight programmes explicitly referenced at least one nutrition-relevant policy, with a total of 16 linked policy/programme pairs (13 pairs for Burkina Faso and 3 for Nigeria) across health, nutrition, agriculture and social focus areas. However, none of the linked pairs were assessed to have complete external coherence, suggesting that priorities at the policy level are not fully realized nor translated at the programme level. This study offers a new approach for the assessment of policy and programme coherence and specifically examines policy and programme linkages. We conclude that improved leadership on country priority setting and better alignment for nutrition within and across sectors is needed to enhance the effectiveness of nutrition investments.
Characterization of an Anti-Ebola Virus Hyperimmune Globulin Derived From Convalescent Plasma
Authors: Ciencewicki Jonathan M , Herbert Andrew S , Storm Nadia , Josleyn Nicole M , Huie Kathleen E , McKay Lindsay G A , Griffiths Anthony , Dye John M , Willis Todd , Arora Vikram 
Published: August 2021

Abstract

Background
Convalescent plasma has been used to treat many viral diseases including Ebola. The manufacture of a purified anti-Ebola virus (EBOV) intravenous immunoglobulin (IVIG) from pooled convalescent plasma is described in this paper.

Methods
An enzyme-linked immunosorbent assay (ELISA) targeting an EBOV surface glycoprotein antigen was used to determine the immunoglobulin titer of pooled plasma and purified anti-EBOV IVIG. Anti-EBOV IVIG was also tested in neutralization assays using a vesicular stomatitis virus pseudovirion expressing EBOV glycoprotein on its surface and with live EBOV. Finally, the efficacy of the anti-EBOV IVIG was assessed in a mouse model of EBOV infection.

Results
In the ELISA, the anti-EBOV IVIG was shown to have a 7-fold increase in immunoglobulin G (IgG) titer over pooled convalescent plasma. In both the pseudovirion and live virus assays, the anti-EBOV IVIG showed approximately 5- to 6-fold increased potency over pooled plasma. Anti-EBOV IVIG also significantly improved survivability in mice infected with the virus when administered concurrently or 2 days after infection.

Conclusions
These data support this purified anti-EBOV IVIG merits additional investigation and clinical trials for treatment and postexposure prophylaxis of Ebola virus disease. The experience gained can be applied to manufacture hyperimmune globulins against other emerging viruses.
A drug candidate for Alzheimer’s and Huntington’s disease, PBT2, can be repurposed to render Neisseria gonorrhoeae susceptible to natural cationic antimicrobial peptides
Authors: Jen Freda E -C , El-Deeb Ibrahim M , Zalucki Yaramah M , Edwards Jennifer L , Walker Mark J , von Itzstein Mark , Jennings Michael P 
Published: August 2021

Abstract

Background
Neisseria gonorrhoeae is a Gram-negative bacterial pathogen that causes gonorrhoea. No vaccine is available to prevent gonorrhoea and the emergence of MDR N. gonorrhoeae strains represents an immediate public health threat.

Objectives
To evaluate whether PBT2/zinc may sensitize MDR N. gonorrhoeae to natural cationic antimicrobial peptides.

Methods
MDR strains that contain differing resistance mechanisms against numerous antibiotics were tested in MIC assays. MIC assays were performed using the broth microdilution method according to CLSI guidelines in a microtitre plate. Serially diluted LL-37 or PG-1 was tested in combination with a sub-inhibitory concentration of PBT2/zinc. Serially diluted tetracycline was also tested with sub-inhibitory concentrations of PBT2/zinc and LL-37. SWATH-MS proteomic analysis of N. gonorrhoeae treated with PBT2/zinc, LL-37 and/or tetracycline was performed to determine the mechanism(s) of N. gonorrhoeae susceptibility to antibiotics and peptides.

Results
Sub-inhibitory concentrations of LL-37 and PBT2/zinc synergized to render strain WHO-Z susceptible to tetracycline, whereas the killing effect of PG-1 and PBT2/zinc was additive. SWATH-MS proteomic analysis suggested that PBT2/zinc most likely leads to a loss of membrane integrity and increased protein misfolding and, in turn, results in bacterial death.

Conclusions
Here we show that PBT2, a candidate Alzheimer’s and Huntington’s disease drug, can be repurposed to render MDR N. gonorrhoeae more susceptible to the endogenous antimicrobial peptides LL-37 and PG-1. In the presence of LL-37, PBT2/zinc can synergize with tetracycline to restore tetracycline susceptibility to gonococci resistant to this antibiotic.
Use of 2-hydroxy-4-(methylthio)-butanoic acid to inhibit Salmonella and Listeria in raw meat for feline diets and palatability in domestic cats
Authors: Owens Tiana G , King Broghan A , Radford Devon R , Strange Philip , Arvaj Laura , Guazzelli Pezzali Julia , Edwards Anne Michelle , Ganesh Daniel , DeVries Trevor J , McBride Brian W , Balamurugan Sampathkumar , Shoveller Anna K 
Published: August 2021

Abstract
While the raw pet food market continues to grow, the risk of bacterial contamination in these types of diets is a major concern, with Salmonella enterica and Listeria monocytogenes being the most frequently associated pathogens in raw pet food product recalls. dl-Methionine is included in some commercial feline kibble and canned diets to improve protein quality; however, an alternative to this is a liquid methionine supplement, 2-hydroxy-4-(methylthio)-butanoic acid (HMTBa), which is also an organic acid. 2-Hydroxy-4-(methylthio)-butanoic acid has previously demonstrated similar efficacy to formic acid against pathogens in a liquid environment and may be a good candidate to inhibit S. enterica and L. monocytogenes in raw ground meat. First, the minimum inhibitory concentration and minimum bactericidal concentration of HMTBa against these pathogens under laboratory growth conditions were determined by measuring growth of pathogens over 36 h when exposed to 10 concentrations of HMTBa (0.10% to 1.00%) mixed with tryptic soy broth. 2-Hydroxy-4-(methylthio)-butanoic acid included at ≥0.50% was bactericidal to S. enterica and L. monocytogenes (P &lt; 0.05). Next, five levels of HMTBa (0.50% to 1.25%) were included in raw ground meat mixtures inoculated with cocktails of S. enterica or L. monocytogenes, and contamination levels were determined at four timepoints: immediately, and after refrigerated storage (4 °C) at 24, 48, and 72 h after removal from freezer (24 h at −20 °C). 2-Hydroxy-4-(methylthio)-butanoic acid included as 1.25% of the meat mixture reduced S. enterica and L. monocytogenes compared with the control (P &lt; 0.05); however, it did not result in total kill of either of these pathogens. Following this, feeding behaviors of seven domestic cats were assessed when offered a raw chicken diet treated with or without 1.25% HMTBa for 5 d each, after which a 2-d 2-choice preference test was conducted. Cats demonstrated a preference for raw diets without HMTBa, but still readily consumed diets with 1.25% HMTBa, suggesting that such a diet was still palatable to them.
A Liberating-Engagement Theory of Consumer Fun
Authors: Oh Travis Tae , Pham Michel Tuan 
Published: August 2021

Abstract
The experience of fun plays a major role in the consumer society. Drawing on a grounded theory approach, we advance a psychological theory of consumer fun. Through an integration of in-depth interviews, narrative analyses, controlled experiments, structural equation modeling, and a photo-ethnography, our multimethod investigation makes four main contributions. First, we show that the experience of fun rests on the combination of two psychological pillars: hedonic engagement and a sense of liberation. Fun is an experience of liberating engagement—a temporary release from psychological restriction via a hedonically engaging activity. Second, we identify four situational facilitators—novelty, social connectedness, spontaneity, and spatial/temporal boundedness—that promote the experience of fun through their effects on hedonic engagement and the sense of liberation. Third, we show that although the psychology of fun is not consumption specific, there is an intimate connection between fun and consumption. Finally, we clarify the relation and distinction between fun and happiness. We discuss implications for our understanding of consumption experiences, business practices related to the engineering of fun, and consumers’ own pursuits of fun and happiness.
Multicancer Early Detection: Learning From the Past to Meet the Future
Authors: Etzioni Ruth , Gulati Roman , Weiss Noel S 
Published: August 2021

Abstract
Multicancer early detection (MCED) tests may soon be available to screen for many cancers using a single blood test, yet little is known about these tests beyond their diagnostic performance. Taking lessons from the history of cancer early detection, we highlight 3 factors that influence how performance of early detection tests translates into benefit and benefit-harm trade-offs: the ability to readily confirm a cancer signal, the population testing strategy, and the natural histories of the targeted cancers. We explain why critical gaps in our current knowledge about each factor prevent reliably projecting the expected clinical impact of MCED testing at this point in time. Our goal is to communicate how much uncertainty there is about the possible effects of MCED tests on population health so that patients, providers, regulatory agencies, and the public are well informed about what is reasonable to expect from this potentially important technological advance. We also urge the community to invest in a coordinated effort to collect data on MCED test dissemination and outcomes so that these can be tracked and studied while the tests are rigorously evaluated for benefit, harm, and cost.