bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting the molecular mechanism of glucosamine in accelerating bone defect repair by stimulating osteogenic proteins
Authors: Gani Maria Apriliani , Nurhan Ahmad Dzulfikri , Budiatin Aniek Setiya , Siswodihardjo Siswandono , Khotib Junaidi 
Published: July 2021

Abstract

Objectives
Bone defect is serious condition that is usually caused by traffic accident. Chitosan is a polymer developed as a scaffold to treat bone defect. However, the mechanism by which chitosan can accelerate bone growth in defect area is still unclear. This study aims to identify proteins which are crucial to the osteogenic properties of chitosan monomer using an in silico study.

Methods
Molecular docking was carried out on chitosan monomer, which are d-glucosamine and glucosamine 6-phosphate units against bone morphogenetic protein 2 (BMP-2), fibronectin, fibroblast growth factor (Fgf), and phosphate transporter (PiT) using AutoDock Vina. Ligand preparation was carried out using Chem3D version 15.0.0.106, while protein preparation was performed using AutoDockTools version 1.5.6.

Results
The results showed that glucosamine 6-phosphate had the best binding affinity with fibronectin and PiT, which was −5.7 kcal mol−1 on both proteins, while d-glucosamine had the best binding affinity with PiT (−5.2 kcal mol−1).

Conclusions
This study suggests that the osteogenic properties of chitosan may be due to the presence of bonds between glucosamine units and fibronectin and/or PiT. However, in vitro studies need to be done to prove this.
Hydration effect on kidney function and serum electrolyte in children with tumor lysis syndrome (TLS) and risk of TLS
Authors: Yulistiani  , Tiffany Claudia , Ugrasena I. Dewa Gede , Qibtiyah Mariyatul 
Published: July 2021

Abstract

Objectives
Tumor lysis syndrome (TLS) is a life-threatening oncology emergency disorder, which may cause acute kidney injury (AKI), arrhythmias, seizures, and sudden death. Hydration is used to prevent TLS in medium-high risk patients, and treatment in TLS patients. According to the pediatric protocol in Dr. Soetomo District General and Teaching Hospital, close monitoring is required to prevent the progression of hematological malignancy towards TLS. The study aimed to analyze the hydration effect on potassium, calcium, and phosphate levels; serum creatinine (sCr); and blood urea nitrogen (BUN) level.

Methods
This was an observational and prospective study conducted at Dr. Soetomo District General and Teaching Hospital for four months on 15 pediatric hemato–oncology patients who got TLS and in risk of TLS. Laboratory parameters were observed in 11 days, pre and post hydration.

Results
Among the 15 patients who met the inclusion criteria, there were eight TLS patients and seven TLS risk patients. After hydration administration 67% of TLS patients achieved normal potassium level, 75% achieved normal phosphate level, 0% achieved normal calcium level, and 50% achieved normal sCr and BUN levels. Meanwhile, TLS risk patients reached normal level in all parameters. This difference in performance is caused by disease progression.

Conclusions
Hydration can maintain serum electrolytes and renal function in a normal range, preventing TLS in TLS risk patients. In TLS patients, hydration only tends slow the progression of the disease.
In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
Authors: Wijayanti Marsih , Ilmi Hilkatul , Kemalahayati Einstenia , Tumewu Lidya , Wardana Fendi Yoga , Suciati  , Hafid Achmad Fuad , Widyawaruyanti Aty 
Published: July 2021

Abstract

Objectives
The rapid spread of antimalarial drug resistance is becoming a problem in the treatment of malaria. The fact was indicated the importance of finding new antimalarial drugs. The genus Garcinia is well known to be a rich source of bioactive prenylated xanthones and triterpenes reported for their antimalarial activity. Garcinia parvifolia is one of the Garcinia genera that can be explored for the search of new antimalarial drugs. This study was aimed to determine the antimalarial activities of G. parvifolia extracts and fractions.

Methods

Garcinia parvifolia Miq. stem was collected from Balikpapan Botanical Garden in East Kalimantan, Indonesia, was extracted gradually with n-hexane, dichloromethane, and methanol by ultrasonic assisted method. The most active extract was further separated using the open column chromatography method. All extracts and fractions were tested against Plasmodium falciparum 3D7 using lactate dehydrogenase (LDH) assay and followed by IC50 determination.

Results
The results showed that all extracts inhibit P. falciparum growth by LDH assay. The highest inhibition was showed by dichloromethane stem extract (BP12-S-D) with the IC50 value of 6.61 ± 0.09 μg/mL. Further fractionation of BP12-S-D has obtained 10 fractions. All of them were identified by TLC, and a brownish-yellow spot (fraction-1) appears after spraying with 10% H2SO4. Fraction-1 (F1) performed the highest parasite growth inhibition with the IC50 value of 6.00 ± 0.03 μg/mL compared with other fractions. This fraction was classified as having a promising activity of antimalarial. The fraction-1 was identified using HPLC, and two major peaks were observed (A and B). The UV–Vis spectra showed the absorption at wavelengths 250 and 278 (A), 243, 281, and 317 nm (B). Based on the profile of TLC, HPLC, and UV–Vis spectra of F1, it was expected that the active compounds are flavonoid (A) and xanthone (B).

Conclusions
The fraction-1 of dichloromethane extract of G. parvifolia Miq. stem has the highest antimalarial activity. It might be a potential candidate for the new antimalarial drug.
Analysis of stress ulcer prophylaxis drug regimentation in surgical patients
Authors: Wijaya Dhani , Suharjono  , Matulatan Fendy , Padolo Elfri 
Published: July 2021

AbstractObjectivesThe World Health Organization (WHO) estimated that more than 50% of drugs were prescribed incorrectly, including stress ulcer prophylaxis (SUP) drugs. Prescribing SUP drugs in incorrect doses and frequencies are considered irrational, and may affects to the effectivity of the therapy. This research aimed to assess the appropriateness of the SUP drugs regimentation in the inpatient surgery room at Dr. Soetomo Hospital, Surabaya, Indonesia.MethodsThis research was cross-sectional study and conducted for 4 weeks in 2019 in the inpatient surgery room of Dr. Soetomo Hospital. The population was SUP drugs that were prescribed in inpatient surgery room. Those SUP drugs with indications for the prevention of stress-induced ulcers that complied to the terms listed on the American Society of Health-System Pharmacists (ASHP) were included as the samples, and vice versa. The samples then assessed for their regimentation appropriateness using the dose and frequency standard of ASHP.ResultsThere were 224 dose units taken as sample, from the total population of 1,404 SUP drugs. The result showed that as much as 48.2% of SUP medications were given to the patients in inappropriate regimentation. Of that number, all ranitidine injection were inappropriately regimented. On the contrary all omeprazole injection dose units were appropriately regimented, meanwhile the amount of appropriate regimentation of sucralfate suspension were 74.6%.ConclusionsAccording to ASHP standard, the SUP drugs in the inpatient surgery room at Dr. Soetomo Hospital were mostly given in inappropriate regimentation. Further research is needed to explore how will those inappropriate regimentation affect on the efficacy of therapy in the patients.
Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves
Authors: Dewi Ika P. , Kwintana Rifdah B. , Ulinnuha Jihan U. , Rachman Fadhillah , Christianty Fransiska M. , Holidah Diana 
Published: July 2021

Abstract

Objectives
The sugarcane leaf is rich inphytochemical content. It is rarely used because it is a waste although it has potential activity as antimutation, anti inflammation, and antioxidation. There is no study about its hepatoprotective activity yet. This study was conducted to determine the hepatoprotection of sugarcane leaves in tested animals with liver acute injury induced by carbon tetrachloride (CCl4).

Methods
Twenty-four Wistar strain rats were divided into three groups of experimental animals (dose 300, 400, and 500 mg/kg) and three control groups (normal, positive, and negative). The ethanol extract of sugarcane leaves obtained from Panti, Jember, was made using the maceration method. The animals were treated for 14 days by giving the extract to the treatment group. One hour after treatment on the last day, the test animals were given CCl4 intraperitoneally except for the normal group. On the 15th day, the blood of the test animal was taken to be tested for the biochemical value of the liver (aspartate transaminase (AST), alanine aminotransferase (ALT), alanine phosphatase (ALP), and bilirubin) and examined for its liver to be made histological preparations.

Results
The results showed that the treatment with a dose of 500 mg/kg was able to decrease AST, ALT, ALP, and bilirubin parameters compared to the negative control. The extract also provided improvements in liver tissue histology compared to the negative control.

Conclusions
Sugarcane leaf ethanol extract (SCLE) has a potential hepatoprotective effect.
Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
Authors: Noh Muhammad Amirul Asyraf , Fazalul Rahiman Siti Sarah , A Wahab Habibah , Mohd Gazzali Amirah 
Published: July 2021

Abstract

Objectives
Tuberculosis (TB) remains a public health concern due to the emergence and evolution of multidrug-resistant strains. To overcome this issue, reinforcing the effectiveness of first line antituberculosis agents using targeted drug delivery approach is an option. Glyceraldehyde-3-Phosphate Dehydrogenase (GADPH), a common virulence factor found in the pathogenic microorganisms has recently been discovered on the cell-surface of Mycobacterium tuberculosis, allowing it to be used as a drug target for TB. This study aims to discover active small molecule(s) that target GAPDH and eventually enhance the delivery of antituberculosis drugs.

Methods
Ten ligands with reported in vitro and/or in vivo activities against GAPDH were evaluated for their binding interactions through molecular docking studies using AutoDock 4.2 program. The ligand with the best binding energy was then modified to produce 10 derivatives, which were redocked against GAPDH using previous protocols. BIOVIA Discovery Studio Visualizer 2019 was used to explore the ligand-receptor interactions between the derivatives and GAPDH.

Results
Among the 10 ligands, curcumin, koningic acid and folic acid showed the best binding energies. Further analysis on the docking of two folic acid derivatives, F7 (γ-{[tert-butyl-N-(6-aminohexyl)]carbamate}folic acid) and F8 (folic acid N-hydroxysuccinimide ester) showed that the addition of a bulky substituent at the carboxyl group of the glutamic acid subcomponent resulted in improved binding energy.

Conclusions
Folic acid and the two derivatives F7 and F8 have huge potentials to be developed as targeting agents against the GAPDH receptor. Further study is currently on-going to evaluate the effectiveness of these molecules in vitro.
Knowledge, attitudes, and practices (KAP) towards COVID-19 among university students in Pakistan: a cross-sectional study
Authors: Faisal Shah , Khotib Junaidi , Zairina Elida 
Published: July 2021

Abstract

Objectives
Pakistan has taken unprecedented measures to control the spread of COVID-19. Complete lockdown followed by smart lockdown and quarantine centres was established. Their awareness and attitude towards COVID-19 had an impact on the individual behaviour of the precautionary measures. The current study examined the knowledge, attitudes and practices of university students in Pakistan.

Methods
An online cross-sectional study was conducted among university students in Pakistan. A questionnaire containing demographic and KAP information related to COVID-19 has been created.

Results
A total of 358 students responded to the survey, and 353 participants completed the study. Among the respondents, 61.5% were male, 76.8% were single, and 58.4% enrolled in a bachelor’s degree. The results showed that most of the respondents (68%) had good knowledge about COVID-19, while the overall knowledge score was 8.78 ± 1.63 (range 1–10). The majority of the respondents (90.9%) were aware of COVID-19, 95.8% knew the sign and symptoms, and 83% of them knew about its transmission. We found a significant difference in knowledge scores across education and area of study p<0.05. More than half (53.5%) of the respondents were satisfied with the facilities provided by the government of Pakistan. The average practices score among the students was 5.08 ± 1.312. A significant difference was found among practice score and area of study p<0.05.

Conclusions
Most of the students have an adequate level of knowledge and are doing better preventive measures against COVID-19. Health education initiatives are required to ensure best practice among the high-risk groups.
Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
Authors: Ardianto Chrismawan , Budiatin Aniek Setiya , Sumartha I Nengah Budi , Nurrahmi Nurrahmi , Rahmadi Mahardian , Khotib Junaidi 
Published: July 2021

Abstract

Objectives
Depression is a mental disorder that profoundly affects all aspects of life, but currently, antidepressants have some problems with their effectiveness and side effects. Resveratrol is a compound that has the ability to regulate the hypothalamic-pituitary-adrenal axis. This study aimed to determine resveratrol’s effect on physical and psychological stress-induced depressive-like behavior.

Methods
Mice were divided into control, physical stress, psychological stress groups. Treatment was conducted with fluvoxamine 20 mg/kg and resveratrol 20, 40, and 80 mg/kg for seven days. The depressive-like state was evaluated using a forced swim test (FST), tail suspension test (TST), and open field test (OFT).

Results
Physical stress and psychological stress induction increase the immobility time on FST and TST. Besides, there is an increase in time in central on OFT, which indicates an anxiety or mental illness-like behavior. However, the OFT examination on sniffing, rearing, grooming, and crossing behavior did not show a significant difference. Resveratrol 80 mg/kg and fluvoxamine 20 mg/kg were significantly reduced immobility time at TST compared to the physical stress group. While in psychological stress, resveratrol 80 mg/kg tended to decrease immobility time but not significant. A significant increase in time in central duration was seen in the resveratrol 40 mg/kg compared to the psychological stress. Stress induction causes increased amygdala corticotrophin-releasing factor (CRF) mRNA expression. However, neither resveratrol nor fluvoxamine affected amygdala CRF mRNA expression.

Conclusions
Resveratrol ameliorates depressive-like behavior induced by physical and psychological stress.
Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant Escherichia coli
Authors: Fahmi Alifia Risma , Suharjono  , Kuntaman  
Published: July 2021

Abstract

Objectives

Escherichia coli is one of the pathogenic bacteria that caused a nosocomial infection. Levofloxacin is one of the fluoroquinolones group antibiotics which is a broad-spectrum antibiotic that works effectively against E. coli. The mutation can happen in the bacteria which caused a resistant effect in the use of antibacterial. This study aimed at identifying mutation in gene gyrA among E. coli that were resistant to levofloxacin.

Methods
The susceptibility of E
. coli was determined by disk diffusion. PCR and sequencing were performed to identify the mutation in gyrA.

Results
A total of 10 isolates showed result resistance to levofloxacin and gyrA gene mutation in the amino acid changes. Nucleotide sequence analysis revealed a point mutation in QRDR (quinolone resistance determining region) of gyrA Ser83→Leu, Asp87→Asn. The silent mutation was also found at codon Val85, Arg91, Ser111, Thr123.

Conclusions
Mutation in the gyrA gene affect the occurrence of bacterial resistance of E. coli to levofloxacin.
Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
Authors: Kurniawati Fivy , Munif Yasin Nanang , Aulia Farida , Vinanda Krisha Gidfrie 
Published: July 2021

Abstract

Objectives
Gastroenteritis is a disease of digestive system commonly occur among the people. Some cases of gastroenteritis are caused by bacteria, so it is treated by using antibiotics. Inappropriate use of antibiotics can be associated to Drug-Related Problems (DRPs). This study aims to identify patterns of potential DRPs of antibiotic use and analyze the effect of potential DRPs of antibiotic use toward the patient’s therapeutic outcomes and length of stay.

Methods
This is a retrospective cross-sectional study carried out by using patient’s medical record. The study population was gastroenteritis patients at the inpatient ward of Universitas Gadjah Mada Hospital during January 2018–June 2019. Then, SPSS was employed to analyze the data and the effect of potential DRPs toward therapeutic outcomes was analyzed by utilizing the chi-square method.

Results
More than half of gastroenteritis patients in Universitas Gadjah Mada Hospital were identified to have potential DRPs of antibiotic use. The most identified of potential DRPs was problems related to drug selection. Based on the chi-square analysis, there was no relationship between potential DRPs of antibiotic use and the therapeutic outcome. In addition, there was also no relationship between potential DRPs of antibiotic use and patient’s length of stay.

Conclusions
The potential DRPs of antibiotics use do not have a significant effect on the therapeutic outcome and length of stay of the gastroenteritis patients in Universitas Gadjah Mada Hospital.
Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
Authors: Machin Abdulloh , Susilo Imam , Purwanto Djoko A. 
Published: July 2021

AbstractObjectivesTo determine the effect of green tea with the active ingredient epigallocathechin-3-gallate (EGCG) on the inhibition of apoptosis in the middle cerebral artery occlusion (MCAO) model.MethodsFour month old maleRattus norvegicusrats with a body weight of 200–275 g was used for the MCAO model and divided into five groups, and the treatment was carried out for 7 days. Before being sacrificed, the subject had 1 cc of blood drawn for high mobility group box 1 (HMGB-1) examination using enzyme-linked immunosorbent assay (ELISA), and after being sacrificed, the brain tissue specimen was taken to examine caspase-3 and B-cell lymphoma 3 (BCL-3) using immunohistochemistry methods.ResultsThere was no significant difference in HMGB-1 results for the treatment group compared to the control group (P1: 384.20 ± 231.72 [p = 0.553]; P2: 379.11 ± 268.4 [p = 0.526]; P3: 284, 87 ± 276.19 [p = 0.140]; P4: 435.32 ± 279.95 [p = 0.912]). There is a significant increase in BCL-2 expression between the treatment group compared to the control group (P1: 2.58 ± 0.51 [p = 0.04]; P2: 3.36 ± 0.50 [p<0.001]; P3: 4.00 ± 0.42 [p<0.001]; P4: 3.60 ± 0.52 [p<0.001]). There was a significant difference in caspase-3 expression compared to the control group in the P3 group (P1: 4.33 ± 0.49 [p = 0.652]; P2: 4.09 ± 0.30 [p = 0.136]; P3: 3.58 ± 0.51 [p = 0.01]; P4: 3.89 ± 0.42 [p = 0.063]). There is no correlation between HMGB-1 and caspase-3 (r = −0.063; p = 0.613) or BCL-2 (r = −0.106; p = 0.396). There is significant negative correlation between caspase-3 and BCL-2 (r = −0.459; p = 0.000).ConclusionsGreen tea with the active ingredient EGCG can inhibit neuronal cell death through the apoptotic pathway and not through the activation of HMGB-1.
Design of gossypetin derivatives based on naturally occurring flavonoid in Hibiscus sabdariffa and the molecular docking as antibacterial agents
Authors: Diyah Nuzul W. , Isnaeni  , Hidayati Shabrina W. , Purwanto Bambang T. , Siswandono  
Published: July 2021

Abstract

Objectives
This study was purposed to design gossypetin derivatives which have higher activity than the parent compound found in Hibiscus sabdariffa and to find the most potent compound as the antibacterial agent.

Methods
Twenty-five gossypetin derivatives were designed by conjugation the molecular structure of gossypetin with acyl group from some natural phenolic acids. The antibacterial activity was predicted by docking simulation on Escherischia coli DNA gyrase (PDB. 1KZN) which was performed by Molegro Virtual Docker. Potency as an antibacterial agent was evaluated based on binding affinity, hydrogen bond, and similarity of binding pattern with reference ligand Clorobiocin.

Results
Almost all derivatives showed higher binding affinity than gossypetin (docking score −113.43 kcal/mol). The most active compound was 3G19 with docking score −167.42 kcal/mol which was comparable to clorobiocin (docking score −167.75 kcal/mol). The compounds displaying higher activity than gossypetin were belonged to 7,4′-dimethyl and 3,7,4′-trimethylgossypetin of coumaric acid, caffeic acid, and also ferulic acid. The compounds showed similar binding mode with clorobiocin especially in interaction with Asn46.

Conclusions
Gossypetin derivatives designed by conjugating the gossypetin with phenolic acyl increased in silico antibacterial activity of the parent compound. The 3,7,4′-trimethylgossypetin of coumaric acid was selected as the most potent compound for antibacterial agents.
Community knowledge and attitude in recognizing asthma symptoms and using medication for asthma attacks: a cross-sectional study
Authors: Puspitasari Arina Dery , Prabawati Bindaria Mutmaina , Rosyid Alfian Nur 
Published: July 2021

Abstract

Objectives
Uncontrolled asthma may be life-threatening. Poor understanding of disease process and appropriate medication use appears to influence community attitude in facing asthmatic patients in an emergency, thereby contributing to increasing the risk of mortality. This study aimed to analyze community-level knowledge about asthma and attitude towards asthma management.

Methods
This observational, cross-sectional study was conducted among the community in Gresik, Indonesia, from March to July 2019. Participants included in this study were adults, who could read, write, and communicate well. Data were collected through questionnaires to evaluate the level of knowledge and attitude towards asthma.

Results
In total, 100 respondents were selected with 91% of women, with a mean age of 49.11 ± 14.42 years and with various levels of education. The respondents had good knowledge by getting a score of 76%. Knowledge regarding recognition of asthma symptoms was scored the highest (83%). However, knowledge about medication use for asthma was lacking, especially in identifying the medicine choice (21%) and inhaler use (48%). The respondents also showed a ‘positive’ attitude with a score of 89%. Most respondents (72%) agreed that when inhaled drugs were unable to relieve the asthma attack, they need to bring the patient to a hospital.

Conclusions
The level of respondent’s knowledge in recognizing asthma symptoms was good, but there were misconceptions about asthma medication, especially in inhaler use. Overall, the respondents had a positive attitude towards asthma perception and management.