bluuebunny/crossref_metadata_2025_split huggingface dataset:
Control of β-Site Amyloid Precursor Protein-Cleaving Enzyme-1 Expression
by Protein Kinase C-λ/ι and Nuclear Factor κ-B
Authors: Farese Robert V. , Sajan Mini P. , Leitges Michael , Park Colin , Diamond David M. , Wu Jin , Hansen Barbara C. , Duncan Mildred A. , Apostolatos Christopher A. , Apostolatos Andre H. , Kindy Mark 
Published: December 2021

Βackground:
β-Amyloid precursor protein-cleaving enzyme-1 (BACE1) initiates the
production of Aβ-peptides that form Aβ-plaque in Alzheimer’s disease.

Methods:
Reportedly, acute insulin treatment in normal mice, and hyperinsulinemia in high-fat-fed
(HFF) obese/diabetic mice, increase BACE1 activity and levels of Aβ-peptides and phospho-
-thr-231-tau in the brain; moreover, these effects are blocked by PKC-λ/ι inhibitors. However, as
chemical inhibitors may affect unsuspected targets, we presently used knockout methodology to
further examine PKC-λ/ι requirements. We found that total-body heterozygous PKC-λ knockout reduced acute stimulatory effects of insulin and chronic effects of hyperinsulinemia in HFF/obese/diabetic mice, on brain PKC-λ activity and production of Aβ1-40/42 and phospho-thr-231-tau. This protection in HFF mice may reflect that hepatic PKC-λ haploinsufficiency prevents the development
of glucose intolerance and hyperinsulinemia.

Results:
On the other hand, heterozygous knockout of PKC-λ markedly reduced brain levels of
BACE1 protein and mRNA, and this may reflect diminished activation of nuclear factor kappa-B
(NFκB), which is activated by PKC-λ and increases BACE1 and proinflammatory cytokine transcription. Accordingly, whereas intravenous administration of aPKC inhibitor diminished aPKC activity and BACE1 levels by 50% in the brain and 90% in the liver, nasally-administered inhibitor
reduced aPKC activity and BACE1 mRNA and protein levels by 50-70% in the brain while sparing
the liver. Additionally, 24-hour insulin treatment in cultured human-derived neurons increased
NFκB activity and BACE1 levels, and these effects were blocked by various PKC-λ/ι inhibitors.

Conclusion:
PKC-λ/ι controls NFκB activity and BACE1 expression; PKC-λ/ι inhibitors may be
used nasally to target brain PKC-λ/ι or systemically to block both liver and brain PKC-λ/ι, to regulate NFκB-dependent BACE1 and proinflammatory cytokine expression.
Assessment of apolipoprotein E genotype for β-amyloid status prediction
Authors: Peña-Bautista Carmen , Álvarez-Sánchez Lourdes , García Lorena , Baquero Miguel , Cháfer-Pericás Consuelo 
Published: December 2021

Background:
Apolipoprotein E (ApoE) is the major genetic risk factor for sporadic
Alzheimer's Disease (AD). Some studies showed a relationship between ApoE4 genotype and the
cerebrospinal fluid (CSF) biomarkers (β-amyloid42, p-Tau, t-Tau), as well as with cognitive status.
In this sense, it could be interesting to develop an approach to establish amyloid status in a minimally invasive way.

Methods:
The present study assessed the ApoE genotype in different participant groups (mild cognitive impairment due to AD (MCI-AD), mild/moderate dementia due to AD, MCI not due to AD
(MCI not AD), other neurological diseases, healthy participants) (n = 342).

Results:
As expected, the ApoE4 allele was more prevalent in AD patients, characterized by impairment in CSF β-amyloid42 levels (Aβ +), than in the other groups (Aβ -). In this sense, ApoE4-carrier subjects showed lower CSF levels for β-amyloid42 and higher CSF levels for t-Tau and p-Tau.
From this, a multivariate model to predict Aβ status was developed by means of partial least square
analysis (PLS) and predictive variables (ApoE genotype, cognitive score, sex, age). This model
showed suitable AUC-ROC 0.792 (95% CI, 0.744-0.840) and predictive negative value (81.6%).

Conclusion:
ApoE genotype could be useful in detecting CSF β-amyloid42 impairment associated
with early AD development.
Subcellular Proteomic Analysis Reveals Dysregulation in the Organization
of Human A549 Cells Infected with Influenza Virus H7N9
Authors: Song Zhigang , Zhang Lijun , Yin Lin , Liu Siyuan , Shi Huichun , Feng Yanling , Zhang Yujiao , Wu Dage 
Published: December 2021

Background:
H7N9 influenza virus poses a high risk to human beings, and proteomic
evaluations of this virus may help better understand its pathogenic mechanisms in human systems.

Objective:
This study aimed at determining membrane proteins related to H7N9 infection.

Methods:
In this study, we infected primary human alveolar adenocarcinoma epithelial cells
(A549) with H7N9 (including wild and mutant strains) and then produced enriched cellular membrane
isolations, which were then evaluated by western blot. The proteins in these cell membrane
fractions were analyzed using the isobaric Tags for Relative and Absolute Quantitation (iTRAQ)
proteome technologies.

Results:
Differentially expressed proteins (n = 32) were identified following liquid chromatography-
tandem mass spectrometry, including 20 down-regulated proteins, such as CD44 antigen and
CD151 antigen, and 12 up-regulated proteins, such as tight junction protein ZO-1 and prostaglandin
reductase 1. Gene Ontology database searching revealed that 20 out of the 32 differentially
expressed proteins were localized to the plasma membrane. These proteins were primarily associated
with the cellular component organization (n = 20) and enriched in the reactome pathway of extracellular
matrix organization (n = 4).

Conclusion:
These findings indicate that H7N9 may dysregulate cellular organization via specific
alterations to the protein profile of the plasma membrane.
Anti-synthetase Syndrome Complicated by Pyogenic Myositis
Authors: Lizarazo David , Cifuentes Karen , Forero Paula Andrea , Páez Hernan 
Published: December 2021

Background:
Anti-synthetase syndrome is a rare autoimmune disorder characterized by
autoantibodies against aminoacyl-tRNA-synthetases. Inflammatory myopathy and interstitial lung
disease could be present among other manifestations. Anti-Jo-1 is the most common antisynthetase
antibody and is the most likely to present with the classic triad (interstitial lung disease, myositis,
and arthritis), and includes more muscle and joint involvement than patients with other antisynthetase
antibodies.

Case report:
Here, we present a case of a 60-year-old female patient with a previous diagnosis of
myositis, secondary to the anti-synthetase syndrome, a complication of pyogenic myositis.

Conclusion:
The diagnosis of anti-synthetase syndrome is made by a multidisciplinary approach,
and occasionally, muscle and/or lung biopsy is needed. Imaging studies, especially magnetic resonance
imaging, based on findings such as muscle and fascial edema, and fatty tissue replacement,
allow an optimal approach.
Neutralizing Aminesin Boiler Steam and Humidified Indoor Air
Authors: Gomes Andrew , Tao Ye , Bhuiyan Abdul , Guan Don , Memarzadeh Farhad 
Published: December 2021

Objective:
The health and comfort of the indoor population have been impacted by the humidity. While people are increasingly spending more time indoors, the presence of pollutants and lack of proper humidification in indoor air have significant risk factors that may lead to multiple health problems.

Methods:
By applying the right air purification and/or humidification system, the adverse effects of poor indoor air quality can be minimized. Direct steam addition is a common technique to humidify the indoor air of a building. Carbon dioxide or other acidic process contaminants may dissolve in steam or steam condensate that will consequently decrease the pH and make it corrosive for the steam distribution system. To avoid that, neutralizing amines are added to steam that keeps the pH neutral or slightly alkaline(pH 7.5-9.0). However, neutralizing amines have some toxic effects. OSHA, FDA, and NIOSH prescribe maximum concentration limits for them in indoor air. The Central Utility Plant (CUP) of National Institutes of Health (NIH) uses a 50:50 blend of cyclohexylamine and diethylaminoethanol as neutralizing agents for the steam.

Results:
The CUP at NIH presents the results of monitoring the concentration level of amines through mass balance, online monitoring at specified location of the steam distribution system and field measurement in humidified indoor air at buildings on campus.

Conclusion
Through theoretical calculation backed up by field measurement, the amine concentration of indoor air is determined to be well below the FDA regulated limits.
Agro-Waste Sourced Catalyst as an Eco-Friendly and Sustainable Approach
for Knoevenagel Condensation Reaction
Authors: Kamanna Kantharaju , Badiger Krishnappa B. , Khatavi Santosh Y. , Hiremath Prashant B. 
Published: December 2021

Background:
The present work describes an eco-friendly and sustainable approach for
the Knoevenagel condensation of an aromatic aldehyde with ethyl cyanoacetate, and salicylaldehyde
with Meldrum acid for the synthesis of ethyl benzylidenecyanoacetate and 3-carboxy coumarin
(2-oxo-2H-1-benzopyran) derivatives, respectively. The reaction was performed under green
catalytic media-Water Extract of Watermelon Fruit Peel Ash (WEWFPA), which is an eco-friendly
protocol derived from the agro-waste feedstock. Various protocols have been reported for the synthesis
of Knoevenagel condensation reaction using a hazardous catalyst or/and solvents found toxic
to the environment, requiring longer reaction time, giving poor yield, and requiring purification of
the final product. The method at hand provides several added advantages like: being a completely
green method, economic, inexpensive catalyst, and the final product isolated is in pure form with
good yield.

Objective:
The objective of the study was to develop a green methodology for the synthesis of
ethyl benzylidenecyanoacetate and 3-carboxy coumarin derivatives.

Results:
The agro-waste based catalyst developed in the present study avoids the use of external inorganic/
organic bases and additives. Knoevenagel condensation of ethyl benzylidenecyanoacetate
and 3-carboxy coumarin derivatives is carried out under room temperature using microwave irradiation,
which is a solvent-free synthesis, requiring less time and giving better yield.

Methods:
We have demonstrated that WEWFPA can be employed as a green homogenous agrowaste
for the synthesis of ethyl benzylidenecyanoacetate and 3-carboxy coumarin derivatives under
rt stirring and microwave irradiation in a very economical way. The developed method is found
to be simple and robust, non-hazardous and solvent-free to obtain the target product.

Conclusion:
In conclusion, we have established an efficient, simple, agro-waste based catalytic approach
for the synthesis of ethylbenzylidenecyanoacetate and 3-carboxy coumarin derivatives employing
WEWFPA as an efficient catalyst under rt stirring and microwave synthesis. The method
is a green, economical and eco-friendly approach for the synthesis of Knoevenagel condensation
products. The advantages of the present approach are that the reaction is a solvent-free synthesis, requiring
no external metal catalyst, chemical base free, short reaction time and excellent yield of
product. The catalyst is agro-waste derived, which is abundant in nature, thus making the present
approach a greener one.
The responsibility for performing civil service of the local civil servants in Vietnam
Authors: Trung Ngo Sy 
Published: December 2021

The study was carried out by qualitative method through collecting secondary data, combined with quantitative method through surveying opinions of 450 managers of 250 representative commune-level government agencies for three regions of the country. Research results have confirmed: Attitude to serve the people (ASP) plays the most important role and has the strongest influence on the responsibilities for performing civil service of civil servants; Next is work responsibility (WR) and work consciousness (WC). From the results of this study, the author suggests some contents that need to be researched and adjusted for managers to improve the quality of local civil servants to meet the requirements of serving the people.
Mobile Device Ownership, Current Use, and Interest in Mobile Health Interventions Among Low-Income Older Chinese Immigrants With Type 2 Diabetes: Cross-sectional Survey Study
Authors: Hu Lu , Trinh-Shevrin Chau , Islam Nadia , Wu Bei , Cao Shimin , Freeman Jincong , Sevick Mary Ann 
Published: December 2021

Background
Chinese immigrants suffer a disproportionately high type 2 diabetes (T2D) burden and tend to have poorly controlled disease. Mobile health (mHealth) interventions have been shown to increase access to care and improve chronic disease management in minority populations. However, such interventions have not been developed for or tested in Chinese immigrants with T2D.

Objective
This study aims to examine mobile device ownership, current use, and interest in mHealth interventions among Chinese immigrants with T2D.

Methods
In a cross-sectional survey, Chinese immigrants with T2D were recruited from Chinese community centers in New York City. Sociodemographic characteristics, mobile device ownership, current use of social media software applications, current use of technology for health-related purposes, and interest in using mHealth for T2D management were assessed. Surveys were administered face-to-face by bilingual study staff in the participant’s preferred language. Descriptive statistics were used to characterize the study sample and summarize technology use.

Results
The sample (N=91) was predominantly female (n=57, 63%), married (n=68, 75%), and had a high school education or less (n=58, 64%); most participants had an annual household income of less than US $25,000 (n=63, 69%) and had limited English proficiency (n=78, 86%). The sample had a mean age of 70 (SD 11) years. Almost all (90/91, 99%) participants had a mobile device (eg, basic cell phones, smart devices), and the majority (n=83, 91%) reported owning a smart device (eg, smartphone or tablet). WeChat was the most commonly used social media platform (65/91, 71%). When asked about their top source for diabetes-related information, 63 of the 91 participants (69%) reported health care providers, followed by 13 who reported the internet (14%), and 10 who reported family, friends, and coworkers (11%). Less than one-quarter (21/91, 23%) of the sample reported using the internet to search for diabetes-related information in the past 12 months. About one-third of the sample (34/91, 37%) reported that they had watched a health-related video on their cell phone or computer in the past 12 months. The majority (69/91, 76%) of participants reported interest in receiving an mHealth intervention in the future to help with T2D management.

Conclusions
Despite high mobile device ownership, the current use of technology for health-related issues remained low in older Chinese immigrants with T2D. Given the strong interest in future mHealth interventions and high levels of social media use (eg, WeChat), future studies should consider how to leverage these existing low-cost platforms and deliver tailored mHealth interventions to this fast-growing minority group.
Investigating Adolescents’ Video Gaming and Gambling Activities, and Their Relationship With Behavioral, Emotional, and Social Difficulties: Protocol for a Multi-Informant Study
Authors: Cena Loredana , Rota Matteo , Trainini Alice , Zecca Sara , Bonetti Zappa Sofia , Tralli Nella , Stefana Alberto 
Published: December 2021

Background
Growing empirical evidence suggests that adolescents have a relatively greater propensity to develop problematic video gaming or gambling habits.

Objective
The main objectives of this study are to estimate the prevalence of potential pathological gambling and video game use among adolescent students and to evaluate their risk factors.

Methods
This is a cross-sectional multi-informant study based on an online survey. It will include a sample of adolescents attending secondary schools located in Brescia, northern Italy, their schoolteachers, and parents. The survey includes extensive data on adolescents’ (1) demographic, social, economic, and environmental characteristics; (2) behavioral, emotional, and social problems and adaptive functioning; (3) emotional and social loneliness; (4) perception of the reasons to use social networks; (5) video game habits and pathological use of video gaming; and (6) gambling behaviors.

Results
This protocol was approved by the Institutional Ethics Board of the Spedali Civili of Brescia (Italy). We expect to collect data from 793 or more adolescent students, as determined by our sample size calculation.

Conclusions
This multisite project will make a substantial contribution to (1) the implementation of a system for identifying pathological gambling and pathological video game use among adolescents, allowing for interventions aimed at improving adolescents’ financial, emotional, and social well-being; and (2) the identification of distinct profiles of gamblers and pathological video gamers that will contribute to setting up effective targeted prevention measures. Understanding the causes and impact of gambling and pathological video gaming on adolescents is a public health issue.

International Registered Report Identifier (IRRID)
DERR1-10.2196/33376
Product Brokering Efficiency as a mediator of Online Product Recommendation and Customer Loyalty
Authors: Lilian Felisa , Elgeka Honey Wahyuni Sugiharto , Hariyanto V Heru 
Published: December 2021

Marketing strategies in e-commerce have a main goal, that is to pursue customer loyalty. Sociolla is an e-commerce company that sells cosmetic products and has a recommendation feature to make it easier for customers during the shopping process. These recommendations can trigger customer satisfaction and generate loyalty. The purpose of this study was to examine the correlation between online product recommendation and customer loyalty with product brokering efficiency as a mediator. 179 Sociolla customers were recruited in this study using convenience sampling. The data were analyzed using the SPSS-Process Hayes model 4. Results showed that perceived decision quality acts as a mediator in the relationship between enablers and customer loyalty (β = .20, [ .13; .27]). It can be concluded that recommendations that are comprehensive, clear, and meet the customer needs will make it easier for customers to make purchasing decisions, which ultimately leads the customers to form loyalty toward the products.
Un método para valorar la inclusión comunitaria en programas sociales
Authors: Téllez Silva José Merced , Gómez Demetrio William , Herrera Tapia Francisco 
Published: December 2021

<span>La integración de la participación comunitaria y social (PCS) en la implementación de la política social mexicana se basa en principios de inclusión y en las buenas prácticas de gobernanza, para mejorar la operatividad y apropiación de la base social sobre las políticas públicas. Con esto se abre un marco de oportunidad teórico-metodológico para caracterizar y valorar dicha acción social. Este aporte muestra un método para analizar la PCS que se instauró en los programas inclusivos más importantes de la Cruzada Nacional contra el Hambre (CNcH), una estrategia con la cual la administración federal mexicana (2012-2018) buscó disminuir la pobreza multidimensional en la población más vulnerable. Utilizando información cualitativa recopilada a través de herramientas de investigación sociológica abocadas al estudio de las instituciones, se obtuvieron referentes que permitieron valorar la PCS y se identificaron áreas de oportunidad para la evaluación de programas sociales implementados en localidades altamente susceptibles a la carencia alimentaria y con acentuados índices de marginación y pobreza.</span>
Instrumentos para Evaluar la Anorexia Nerviosa
Authors: Rivas Teresa 
Published: December 2021

Este artículo aborda el estudio de los instrumentos más utilizados en la evaluación de la Anorexia Nerviosa (AN) en población de habla hispana, perteneciendo este trastorno a los Trastornos de la Conducta Alimentaria (TCA). Es suficientemente conocido que la prevalencia de este tipo de trastornos ha ido en aumento a lo largo de la historia, y más en población femenina y en sociedades industrializadas, en las que el logro y la aceptación está muy relacionado con estar delgado o sentirse atractivo (Salazar, 2008). Todo esto ha llevado a la realización de diversos estudios de estimación de la prevalencia de los TCA. Rivas, Bersabé y Castro (2001a) en una muestra de 1,555 adolescentes españoles de entre 12 y 21 años de ambos géneros (625 varones y 930 mujeres), obtuvieron los porcentajes 1.1% y 4.0% para TCA, 0.5% y 0.4% para AN, 0.2% y 0.6% para Bulimia Nerviosa (BN), 0.5% y 3.9% para Trastorno de la Conducta Alimentaria No Especificado (TCANE), en varones y mujeres, respectivamente. Otros estudios de prevalencia, en dos etapas, se han realizado en muestras de población general de mujeres adolescentes o adolescentes tempranas españolas (e.g. Pérez-Gaspar et al., 2000; Ruiz-Lázaro et al., 2010). En México se desconocen datos de prevalencia en población masculina y/o que proporcionen la prevalencia en cada uno de los TCA. Los datos encontrados sobre la prevalencia de los TCA en población femenina mexicana establecen que un 0.2% padecen AN y un 0.9% BN (Benjet et al., 2008). También se han realizado diversos estudios transculturales entre México y España encontrándose mayores porcentajes de sintomatología de TCA en mujeres españolas (Raich et al., 2001; Toro et al., 2006). No obstante, las mujeres mexicanas tienen un número mayor de conductas de riesgo, por ejemplo: insatisfacción con su cuerpo (Gómez-Peresmitré y Acosta, 2000) y, preocupación por el peso y deseo de estar delgadas (Gómez-Peresmitré y Acosta, 2002).
CT-severity Score in COVID-19 patients, Assessment of Performance in Triage and Outcome Prediction: a Comparative Study of Different Methods
Authors: Nokiani Alireza Almasi 
Published: December 2021

BACKGROUND: Lung involvement in COVID-19 can be quantified by
chest CT scan with some triage and prognostication value. At least 7
CT-severity score (CTSS) systems have been proposed. PURPOSE:
We evaluated triage and prognostication performance of seven different
CTSSs for COVID-19. MATERIALS AND METHODS: COVID-19, PCR
positive patients admitted from February 20th 2020 to July
22 were included into a retrospective study.
Demographic data and clinical data indicating disease severity at
presentation and in peak disease severity were recorded. CT images were
reviewed and scored according to seven different scoring systems
(CTSS1-CTSS7) by two radiologists. Interrater reliability was determined
for each CTSS. Then clinical severity of the disease at presentation
(for triage) and peak disease severity (for outcome) were compared with
CTSSs separately. ROC curves for performance of each CTSS in diagnosing
severe/critical disease on admission, severe/critical disease at peak
disease severity and critical disease at peak severity were plotted.
Areas under the curve (AUCs), best thresholds and corresponding
sensitivities and specificities were calculated. RESULTS: 96
patients were included with mean age of 63.6 ± 17.4 years (range: 21-88,
median: 67). 57 (59,4%) were men and 39 (40.6%) were women. All CTSSs
showed good interrater reliability as calculated intraclass correlation
coefficients (ICCs) were 0.764-0.837 for all of the CTSSs. Only three
CTSSs showed acceptable AUCs (AUC =0.7) for triage of severe/critical
patients. All CTSSs showed acceptable AUCs for prognostication
(AUCs=0.76-0.79). Calculated AUCs were not significantly different for
triage and for prediction of severe/critical disease but some difference
was shown for prediction of critical disease. CONCLUSION: Men
are probably affected more frequently than women by COVID19. CTSS
performance in triage was much lower than earlier reports and only three
CTSSs showed acceptable AUCs. CTSS performed better for prognostic
purposes than for triage as all 7 CTSSs showed acceptable AUCs in both
types of prognostic ROC curves. Our results are compatible with those of
recent studies. There is not much difference among performance of
different CTSSs.