bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells
Authors: Cojocaru Florentina , Şelescu Tudor , Domocoş Dan , Măruţescu Luminiţa , Chiritoiu Gabriela , Chelaru Nicoleta-Raluca , Dima Simona , Mihăilescu Dan , Babes Alexandru , Cucu Dana 
Published: January 2021

AbstractThe transient receptor potential ankyrin type 1 (TRPA1) channel belongs to the TRP superfamily of ion channels. TRPA1 is a membrane protein with multiple functions able to respond to noxious stimuli, reactive oxygen species, inflammatory cytokines or pungent substances, and it participates in pain signalling, taste, inflammation and various steps of the tumorigenic process. To date, no reports have addressed the expression and function of TRPA1 in pancreatic ductal adenocarcinoma (PDAC) cells. This work reports the endogenous expression of TRPA1 channels in human pancreatic adenocarcinoma cell lines and provides insights into the function of the TRPA1 protein in the Panc-1 cell line. This study reports that cell lines isolated from PDAC patients had different levels of TRPA1 expression. The channel activity in Panc-1 cells, as assessed with electrophysiological (whole-cell patch clamp) and microfluorimetry methods, showed that non-selective cationic currents were activated by allyl isothiocyanate (AITC) in Panc-1 cells and inhibited by the selective TRPA1 antagonist A-967079. The current elicited by the specific agonist was associated with a robust increase in intracellular Ca2+. Furthermore, siRNA-induced downregulation of TRPA1 enhanced cell migration in the wound healing assay, indicating a possible role of ion channels independent from pore function. Finally, TRPA1 activation changed the cell cycle progression. Taken together, these results support the idea of channel-dependent and independent role for TRPA1 in tumoral processes.
Controlling soil disturbance of a lunar regolith simulant bed during depressurization in a vacuum chamber
Authors: Go Gyu-Hyun , Lee Jangguen , Chung Taeil , Ryu Byung Hyun , Jin Hyunwoo , Zhuang Li , Shin Hyu Soung , Kim Jae Hyun , Yun Tae Sup 
Published: January 2021

AbstractA dusty thermal vacuum chamber (DTVC) containing a regolith simulant bed is essential for testing equipment and techniques related to lunar surface exploration. Space agencies have been reluctant to operate a DTVC because of the challenge of controlling soil disturbance of the lunar regolith simulant bed during pumping down or depressurization, which may contaminate or even damage the chamber and vacuum equipment. There appears to be no previously available solution to this problem, or how to avoid it. We investigated the mechanism of soil disturbance during depressurization and established a criterion for evaluating its occurrence. The proposed criterion was validated by extensive experiments and numerical modelling to simulate air evacuation from soil voids. There is a critical pressure difference (CPD) between the top and bottom of the lunar regolith simulant bed that causes soil disturbance during depressurization. We found a simple equation estimating the CPD and further provided guideline on the optimum depressurization rate to avoid soil disturbance before the target vacuum level is achieved under varying soil conditions.
Secondary electron emission under magnetic constraint: from Monte Carlo simulations to analytical solution
Authors: Costin Claudiu 
Published: January 2021

AbstractThe secondary electron emission process is essential for the optimal operation of a wide range of applications, including fusion reactors, high-energy accelerators, or spacecraft. The process can be influenced and controlled by the use of a magnetic field. An analytical solution is proposed to describe the secondary electron emission process in an oblique magnetic field. It was derived from Monte Carlo simulations. The analytical formula captures the influence of the magnetic field magnitude and tilt, electron emission energy, electron reflection on the surface, and electric field intensity on the secondary emission process. The last two parameters increase the effective emission while the others act the opposite. The electric field effect is equivalent to a reduction of the magnetic field tilt. A very good agreement is shown between the analytical and numerical results for a wide range of parameters. The analytical solution is a convenient tool for the theoretical study and design of magnetically assisted applications, providing realistic input for subsequent simulations.
Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
Authors: Coelho Vitor Lima , de Brito Tarcísio Fontenele , de Abreu Brito Ingrid Alexandre , Cardoso Maira Arruda , Berni Mateus Antonio , Araujo Helena Maria Marcolla , Sammeth Michael , Pane Attilio 
Published: January 2021

AbstractRhodnius prolixusis a Triatominae insect species and a primary vector of Chagas disease. The genome ofR. prolixushas been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5′ and 3′ UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes inR. prolixusfrom 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles duringR. prolixusoogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve theR. prolixusgenome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.
Design and fabrication of dual responsive lignin-based nanogel via “grafting from” atom transfer radical polymerization for curcumin loading and release
Authors: Dinari Ali , Abdollahi Mahdi , Sadeghizadeh Majid 
Published: January 2021

AbstractThe story of human dreams about curing all diseases, disorders and lesions is as old as human history. In the frontier of medical science, nanomedicine is trying to solve the problem. In this study, inspired by nanotechnology and using “grafting from” approach, a novel lignin-based nanogel was synthesized using atom transfer radical polymerization (ATRP) method. N-isopropylacrylamide (NIPAM) and N,N-dimethylaminoethylmethacrylate (DMAEMA) comonomers were graft copolymerized from fully brominated lignin as ATRP macroinitiator to synthesize lignin-g-P(NIPAM-co-DMAEMA) nanogel (LNDNG). By controlling the initial comonomer compositions and ATRP conditions, four LNDNG systems with different lower critical solution temperatures (LCSTs) of 32, 34, 37 and 42 °C were prepared. The LNDNGs were evaluated by GPC, FT-IR, 1H NMR, UV–Vis, DLS, SEM and TEM analyses. The prepared nanogels exhibited an average diameter of 150 nm with dual temperature and pH responsiveness. Curcumin (CUR) loading capacity and encapsulation efficiency of the LNDNGs were 49.69% and 92.62% on average, respectively. The cumulative release amount of loaded CUR was observed to be 65.36% after 72 h. The new lignin-based NGs proposed in the present work seems to be a promising, safe and comparable system in a near future.
Population genetics and forensic utility of 23 autosomal PowerPlex Fusion 6C STR loci in the Kuwaiti population
Authors: Haidar Mahdi , Abbas Fatimah A. , Alsaleh Hussain , Haddrill Penelope R. 
Published: January 2021

AbstractThis study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy–Weinberg equilibrium, nor any linkage disequilibrium between and within loci, indicating that these loci are inherited independently, and their allele frequencies can be used to estimate match probabilities in the Kuwaiti population. The low combined match probability of 7.37 × 10–30 and the high paternity indices generated by these loci demonstrate the usefulness of the PowerPlex Fusion 6C kit for human identification in this population, as well as to strengthen the power of paternity testing. Off-ladder alleles were seen at several loci, and these were identified by examining their underlying nucleotide sequences. Principal component analysis (PCA) and STRUCTURE showed no genetic structure within the Kuwaiti population. However, PCA revealed a correlation between geographic and genetic distance. Finally, phylogenetic trees demonstrated a close relationship between Kuwaitis and Middle Easterners at a global level, and a recent common ancestry for Kuwait with its northern neighbours of Iraq and Iran, at a regional level.
Relationship of breast volume, obesity and central obesity with different prognostic factors of breast cancer
Authors: Lubián López Daniel María , Butrón Hinojo Carmen Aisha , Castillo Lara María , Sánchez-Prieto Manuel , Sánchez-Borrego Rafael , Mendoza Ladrón de Guevara Nicolas , González Mesa Ernesto 
Published: January 2021

AbstractThe objective of this study was to investigate whether the BC tumor biology in women with larger breast volume, in obese women and especially in women with central adiposity at the moment of diagnosis of BC is more aggressive than in those women without these characteristics. 347 pre- and postmenopausal women with a recent diagnosis of BC were analyzed. In all patients, anthropometric measurements at the time of diagnosis was collected. In 103 of them, the breast volume was measured by the Archimedes method. The Breast volume, BMI, WHR and the menopausal status were related to different well-known pathological prognostic factors for BC. At the time of diagnosis, 35.4% were obese (BMI > 30 kg/m2), 60.2% had a WHR ≥ 0.85, 68.8% were postmenopausal and 44.7% had a breast volume considered "large" (> 600 cc). Between patients with a large breast volume, only a higher prevalence of ER (+) tumors was found (95.3% vs. 77.2%; p = 0.04) compared to those with small breast volumes. The obese BC patients showed significantly higher rates of large tumors (45.5% vs. 40.6%; p = 0.04), axillary invasion (53.6% vs. 38.8%; p = 0.04), undifferentiated tumors (38.2% vs. 23.2%) and unfavorable NPI (p = 0.04) than non-obese women. Those with WHR ≥ 0.85 presented higher postsurgical tumor stages (61.7% vs. 57.8%; p = 0.03), higher axillary invasion (39.9% vs. 36.0%; p = 0.004), more undifferentiated tumors (30.0% vs. 22.3%; p = 0.009), higher lymphovascular infiltration (6.5% vs. 1.6%; p = 0.02), and a higher NPI (3.6 ± 1.8 vs. 3.2 ± 1.8; p = 0.04). No statistically significant differences were found according to menopausal status. We conclude that obesity, but especially central obesity can be associated with a more aggressive tumour phenotype. No relation between breast volume and tumoral prognostic factors was found, except for a higher proportion of ER (+) tumor in women with higher breast volume.
Positive expectations predict improved mental-health outcomes linked to psychedelic microdosing
Authors: Kaertner L. S. , Steinborn M. B. , Kettner H. , Spriggs M. J. , Roseman L. , Buchborn T. , Balaet M. , Timmermann C. , Erritzoe D. , Carhart-Harris R. L. 
Published: January 2021

AbstractPsychedelic microdosing describes the ingestion of near-threshold perceptible doses of classic psychedelic substances. Anecdotal reports and observational studies suggest that microdosing may promote positive mood and well-being, but recent placebo-controlled studies failed to find compelling evidence for this. The present study collected web-based mental health and related data using a prospective (before, during and after) design. Individuals planning a weekly microdosing regimen completed surveys at strategic timepoints, spanning a core four-week test period. Eighty-one participants completed the primary study endpoint. Results revealed increased self-reported psychological well-being, emotional stability and reductions in state anxiety and depressive symptoms at the four-week primary endpoint, plus increases in psychological resilience, social connectedness, agreeableness, nature relatedness and aspects of psychological flexibility. However, positive expectancy scores at baseline predicted subsequent improvements in well-being, suggestive of a significant placebo response. This study highlights a role for positive expectancy in predicting positive outcomes following psychedelic microdosing and cautions against zealous inferences on its putative therapeutic value.
Leakage of astrocyte-derived extracellular vesicles in stress-induced exhaustion disorder: a cross-sectional study
Authors: Wallensten Johanna , Nager Anna , Åsberg Marie , Borg Kristian , Beser Aniella , Wilczek Alexander , Mobarrez Fariborz 
Published: January 2021

AbstractPatients with stress-induced exhaustion disorder (SED) demonstrate cognitive dysfunction similar to patients with minor traumatic brain injury (TBI). We have previously detected elevated concentrations of astrocyte-derived extracellular vesicles (EVs) in patients with TBI. As such, we hypothesized that astrocyte-derived EVs could be higher in patients with SED than in patients with major depressive disorder (MDD) and healthy controls. Patients with SED (n = 31), MDD (n = 31), and healthy matched controls (n = 61) were included. Astrocyte-derived EVs (previously known as microparticles) were measured in plasma with flow cytometry and labeled against glial fibrillary acidic protein (GFAP) and aquaporin 4 (AQP4). In addition, platelet EVs and their CD40 ligand expression were measured. Patients with SED had significantly higher concentrations of AQP4 and GFAP-positive EVs and EVs co-expressing AQP4/GFAP than patients with MDD and healthy controls. Patients with MDD had significantly higher concentrations of GFAP-positive EVs and EVs co-expressing AQP4/GFAP than healthy controls. Platelet EVs did not differ between groups. CD40 ligand expression was significantly higher in patients with SED and MDD than in controls. In conclusion, the present study suggests that patients with SED, and to some extent, patients with MDD, have increased leakage of astrocyte-derived EVs through the blood–brain barrier.
Automatic recognition of uterine contractions with electrohysterogram signals based on the zero-crossing rate
Authors: Song Xiaoxiao , Qiao Xiangyun , Hao Dongmei , Yang Lin , Zhou Xiya , Xu Yuhang , Zheng Dingchang 
Published: January 2021

AbstractUterine contraction (UC) is an essential clinical indicator in the progress of labour and delivery. Electrohysterogram (EHG) signals recorded on the abdomen of pregnant women reflect the uterine electrical activity. This study proposes a novel algorithm for automatic recognition of UCs with EHG signals to improve the accuracy of detecting UCs. EHG signals by electrodes, the tension of the abdominal wall by tocodynamometry (TOCO) and maternal perception were recorded simultaneously in 54 pregnant women. The zero-crossing rate (ZCR) of the EHG signal and its power were calculated to modulate the raw EHG signal and highlight the EHG bursts. Then the envelope was extracted from the modulated EHG for UC recognition. Besides, UC was also detected by the conventional TOCO signal. Taking maternal perception as a reference, the UCs recognized by EHG and TOCO were evaluated with the sensitivity, positive predictive value (PPV), and UC parameters. The results show that the sensitivity and PPV are 87.8% and 93.18% for EHG, and 84.04% and 90.89% for TOCO. EHG detected a larger number of UCs than TOCO, which is closer to maternal perception. The duration and frequency of UC obtained from EHG and TOCO were not significantly different (p > 0.05). In conclusion, the proposed UC recognition algorithm has high accuracy and simple calculation which could be used for real-time analysis of EHG signals and long-term monitoring of UCs.
Microplastics in fish and fishmeal: an emerging environmental challenge?
Authors: Thiele Christina J. , Hudson Malcolm D. , Russell Andrea E. , Saluveer Marilin , Sidaoui-Haddad Giovanna 
Published: January 2021

AbstractMicroplastics are contaminants of emerging concern; they are ingested by marine biota. About a quarter of global marine fish landings is used to produce fishmeal for animal and aquaculture feed. To provide a knowledge foundation for this matrix we reviewed the existing literature for studies of microplastics in fishmeal-relevant species. 55% of studies were deemed unsuitable due to focus on large microplastics (> 1 mm), lack of, or limited contamination control and polymer testing techniques. Overall, fishmeal-relevant species exhibit 0.72 microplastics/individual, with studies generally only assessing digestive organs. We validated a density separation method for effectiveness of microplastic extraction from this medium and assessed two commercial products for microplastics. Recovery rates of a range of dosed microplastics from whitefish fishmeal samples were 71.3 ± 1.2%. Commercial samples contained 123.9 ± 16.5 microplastics per kg of fishmeal—mainly polyethylene—including 52.0 ± 14.0 microfibres—mainly rayon. Concentrations in processed fishmeal seem higher than in captured fish, suggesting potential augmentation during the production process. Based on conservative estimates, over 300 million microplastic particles (mostly < 1 mm) could be released annually to the oceans through marine aquaculture alone. Fishmeal is both a source of microplastics to the environment, and directly exposes organisms for human consumption to these particles.
The associations between serum trace elements and bone mineral density in children under 3 years of age
Authors: Wu Ziyi , Yuan Yuhao , Tian Jian , Long Feng , Luo Wei 
Published: January 2021

AbstractWe examined the associations of age and serum magnesium, iron, lead, copper, and zinc levels with bone mineral density (BMD) in 2412 children under 3 years of age in order to find a tool to monitor BMD in children without the use of expensive imaging techniques. One-way ANOVA and chi-square tests were used to determine the associations of age and serum trace elements with BMD. Multivariable logistic regression analysis was used to test the correlation of five serum trace elements with BMD after adjustments for potential confounding factors in children under 3 years of age. Significant associations between age and four serum trace elements and BMD were found. Compared to the group with the lowest serum levels detected, the adjusted odds ratio (OR) for the incidence of normal bone mineral density in the third magnesium concentration tertile, the third iron concentration tertile, the fifth copper concentration quintile, the third zinc concentration quintile, and the fifth zinc concentration quintile were 1.30 (95% confidence interval (CI) 1.02–1.67), 1.43 (95% CI 1.11–1.84), 1.42 (95% CI 1.04–1.94), 1.46 (95% CI 1.05–2.04), and 1.48 (95% CI 1.06–2.06), respectively. However, there was no significant correlation between serum lead level and BMD in this study. Age and serum magnesium, iron, copper, and zinc levels are positively associated with BMD in children under 3 years old.
Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
Authors: Hoang Trung Hieu , Yoshihara Toru , Nakao Akito , Hayashida Katsumi , Hirata Yoshiki , Shirasuna Koumei , Kuwamura Mitsuru , Nakagawa Yuki , Kaneko Takehito , Mori Yasuo , Asano Masahide , Kuramoto Takashi 
Published: January 2021

AbstractThe RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function.
Evaluation of commercially available aroA delated gene E. coli O78 vaccine in commercial broiler chickens under Middle East simulating field conditions
Authors: Galal Hussein M. , Abdrabou M. I. , Faraag Ahmed H. I. , Mah C. K. , Tawfek Azza M. 
Published: January 2021

AbstractThe broiler industry in the Middle East (ME) faces many challenges related to bacterial infections, including M. gallisepticum, M. synoviae, E. coli, and other gram-negative bacteria, exacerbated by various errors in the brooding process. Antibiotics use in the first three days of life, such as Linco-Spectin 100 SP, tilmicosin, enrofloxacin, tylosin, colistin, and doxycycline, is the trend in the market to control such challenges. This study aimed to evaluate the efficacy of the newly introduced aroA E. coli vaccine (Poulvac E. coli) and its ability to reduce over-reliance on the heavy use of antibiotics in the ME. The study was conducted on 160 broiler chicks, divided into eight even groups. Each group was treated differently in terms of antibiotic therapy and ages at the time of Poulvac E. coli administration and the challenge of virulent avian pathogenic E. coli (APEC), serotype O78. Spray application of Poulvac E. coli at seven days of age plus Linco-Spectin 100 SP during the first three days provided the best results for zero mortality after challenge with APEC, while Poulvac E. coli at seven days with enrofloxacin during the early three days resulted in 10% mortality. Poulvac E. coli hatchery vaccination protected birds against mortality but reduced body weight gain compared to the 7-day group vaccinated with Linco-Spectin 100 SP during the first three days. Poulvac E. coli given on day one or day seven did not affect the immune response to concurrent respiratory viral vaccines and, in some cases, improved response. This study shows that Poulvac E. coli at seven days of age, together with Linco-Spectin 100 during the first three days, has produced the best results in terms of protection and performance in the ME high presence of avian pathogenic E. coli field challenge.