bluuebunny/crossref_metadata_2025_split huggingface dataset:
COVID-19 Pandemic Influence on Medical Oncology Provider Perceptions of Telehealth Video Visits
Authors: Handley Nathan R. , Heyer Arianna , Granberg Rachel E. , Binder Adam F. , Gentsch Alexzandra T. , Csik Valerie P. , Garber Gregory , Worster Brooke , Lopez Ana Maria , Rising Kristin L. 
Published: October 2021

PURPOSE: The COVID-19 pandemic necessitated a rapid expansion of telehealth use in oncology, a specialty in which prior utilization was low in part because of barriers perceived by providers. Understanding the changing perceptions of medical oncology providers during the pandemic is critical for continued expansion and improvement of telehealth in cancer care. This study was designed to identify medical oncology providers' perceptions of telehealth video visits as influenced by the COVID-19 pandemic. METHODS: We conducted semi-structured interviews with medical oncology providers from November 20, 2020, to January 27, 2021, at the Sidney Kimmel Cancer Center at Thomas Jefferson University, a National Cancer Institute–designated cancer center in an urban, academic health system in Philadelphia, PA. We assessed provider perceptions of the impact of the COVID-19 pandemic on (1) provider-level comfort and willingness for telehealth, (2) provider-perceived patient comfort and willingness to engage in telehealth, and (3) continued barriers to successful telehealth use. RESULTS: Volunteer and convenience sampling resulted in the participation of 25 medical oncology providers, including 18 physicians and seven advanced practice providers, in semi-structured interviews. Of the 25 participants, 13 (52%) were female and 19 (76%) were White, with an average age of 48.5 years (standard deviation = 12.6). Respondents largely stated an increased comfort level and willingness for use of video visits. In addition, respondents perceived a positive change in patient comfort and willingness, mostly driven by convenience, accessibility, and reduced risk of COVID-19 exposure. However, several reported technologic issues and limited physical examination capability as remaining barriers to telehealth adoption. CONCLUSION: The rapid adoption of telehealth necessitated by the COVID-19 pandemic has increased provider-level and provider-perceived patient comfort and willingness to engage in video visits for cancer care. As both providers and patients increasingly accept telehealth across many use cases, future work should focus on further addressing technology and physical examination barriers and ensuring continued reimbursement for telehealth as a routine part of covered care.
Tumor-Specific and Tumor-Agnostic Molecular Signatures Associated With Response to Immune Checkpoint Inhibitors
Authors: Schlauch Daniel , Fu Xiaotong , Jones Suzanne F. , Burris Howard A. , Spigel David R. , Reeves James , McKenzie Andrew J. 
Published: October 2021

PURPOSENext-generation sequencing (NGS) testing is being incorporated into routine standard of care for patients with cancer. Immune checkpoint inhibitors (CPIs) are approved for use in both tumor-specific and tumor-agnostic indications. We sought to determine tumor type–specific or tumor-agnostic correlations between mutations detected by NGS and response to CPIs.MATERIALS AND METHODSA retrospective analysis of 26,004 patient records with NGS data available was conducted. Time to treatment failure and overall survival analyses were performed. Hazard ratios and associated statistics were computed in the R programming language. The study was considered exempt from internal review board review and data were considered nonhuman subjects.RESULTSResponse to CPIs varied between tumor types with melanoma and lung cancer performing relatively better on CPIs than other tumor types. Within tumor types, response to CPIs was stratified by mutations in specific genes. Tumor-agnostic markers including high tumor mutation burden and microsatellite instability-high were also associated with longer time to treatment failure on CPIs. Importantly, within the high tumor mutation burden and microsatellite instability-high groups, mutations in individual genes correlate with response to CPIs.CONCLUSIONThe results from commercial NGS panels may be used to stratify patients for response to CPIs. In tumors where CPIs show relatively low efficacy, there may be distinct patient populations—based on gene mutation status—that are predicted to have better response to CPIs. Likewise, there may be distinct patient populations who do relatively worse on CPIs within tumor types known to respond well to CPIs.
Formal proof of the requirement of MESP1 and MESP2 in mesoderm specification and their transcriptional control via specific enhancers in mice
Authors: Ajima Rieko , Sakakibara Yuko , Sakurai-Yamatani Noriko , Muraoka Masafumi , Saga Yumiko 
Published: October 2021

ABSTRACT
MESP1 and MESP2 are transcriptional factors involved in mesoderm specification, somite boundary formation and somite polarity regulation. However, Mesp quadruple mutant zebrafish displayed only abnormal somite polarity without mesoderm specification defects. In order to re-evaluate Mesp1/Mesp2 mutants in mice, Mesp1 and Mesp2 single knockouts (KOs), and a Mesp1/Mesp2 double KO were established using genome-editing techniques without introducing selection markers commonly used before. The Mesp1/Mesp2 double KO embryos exhibited markedly severe mesoderm formation defects that were similar to the previously reported Mesp1/Mesp2 double KO embryos, indicating species differences in the function of MESP family proteins. However, the Mesp1 KO did not display any phenotype, including heart formation defects, which have been reported previously. We noted upregulation of Mesp2 in the Mesp1 KO embryos, suggesting that MESP2 rescues the loss of MESP1 in mesoderm specification. We also found that Mesp1 and Mesp2 expression in the early mesoderm is regulated by the cooperation of two independent enhancers containing T-box- and TCF/Lef-binding sites. Deletion of both enhancers caused the downregulation of both genes, resulting in heart formation defects. This study suggests dose-dependent roles of MESP1 and MESP2 in early mesoderm formation.
Cooperation of membrane-translocated syntaxin4 and basement membrane for dynamic mammary epithelial morphogenesis
Authors: Hirose Yuina , Hirai Yohei 
Published: October 2021

ABSTRACT
Mammary epithelia undergo dramatic morphogenesis after puberty. During pregnancy, luminal epithelial cells in ductal trees are arranged to form well-polarized cystic structures surrounded by a myoepithelial cell layer, an active supplier of the basement membrane (BM). Here, we identified a novel regulatory mechanism involved in this process by using a reconstituted BM-based three-dimensional culture and aggregates of a model mouse cell line, EpH4, that had either been manipulated for inducible expression of the t-SNARE protein syntaxin4 in intact or signal peptide-connected forms, or that were genetically deficient in syntaxin4. We found that cells extruded syntaxin4 upon stimulation with the lactogenic hormone prolactin, which in turn accelerated the turnover of E-cadherin. In response to extracellular expression of syntaxin4, cell populations that were less affected by the BM actively migrated and integrated into the cell layer facing the BM. Concurrently, the BM-facing cells, which were simultaneously stimulated with syntaxin4 and BM, acquired unique epithelial characteristics to undergo dramatic cellular arrangement for cyst formation. These results highlight the importance of the concerted action of extracellular syntaxin4 extruded in response to the lactogenic hormone and BM components in epithelial morphogenesis.
Pneumococcal carriage and antibiotic susceptibility patterns in mother-baby pairs in a rural community in Eastern Uganda: a cross-sectional study
Authors: Madut Akech Gabriel , Naloli Mercy , Sebwami Paul , Kazibwe Patrick , Atwikiriize Maureen , Onyait Julius , Oboth Paul , Nteziyaremye Julius , Nekaka Rebecca , Iramiot Jacob Stanley 
Published: October 2021

Background: Pneumococcal carriage predisposes children to pneumonia. Pneumonia poses a significant threat to the lives of children below five years old worldwide, contributing to a high number of hospitalizations and death. Morbidity and morbidity are especially common in children under five and the elderly, although any age group can be affected. This study aimed to estimate pneumococcal carriage and determine antibiotic susceptibility patterns of the pneumococci isolated from mother-baby pairs in Ngora district after the rollout of the pneumococcal vaccine. We hypothesized that high carriage of Streptococcus pneumoniae in mothers leads to carriage in their babies and hence a greater chance of contracting pneumonia. Methods: Consecutive sampling was used to select 152 mother-baby pairs from community visits and those seeking care at the health facility. We collected nasal swabs from both baby and mother for culture and sensitivity testing using Kirby-Bauer’s agar disc diffusion method. Data was also collected from the mothers who consented to take part in the study, using an interviewer-administered questionnaire. Results: This study found that there was a low prevalence of pneumococcal carriage in the mother-baby pair in the Ngora district. Only one mother-baby pair (1/152) was found to be colonized with pneumococci in both mother and baby and the rest of S. pneumoniae colonized either the mother or baby. We also observed high rates of microbial resistance to penicillin, which is the first-line drug for the management of pneumonia in Uganda. Also, high resistance patterns were recorded with chloramphenicol (50%) and tetracycline (50%), whereas the lowest resistance was recorded in clindamycin (17%). Conclusions: The relationship between pneumococcal carriage and immunization status suggests that the pneumococcal vaccine is protective against the pneumococcal carriage. Resistance of S. pneumoniae to commonly used antibiotics was high.
Optimal design for a psychosocial intervention on severely acute malnourished children in humanitarian settings: results of an expert survey
Authors: N'DIAYE Dieynaba S , Salpéteur Cécile , Bizouerne Cécile , Le Roch Karine 
Published: October 2021

Background: Common psychosocial interventions focus on feeding, stimulation, emotional responsiveness and childcare and parenting. Inclusion of such a component in the outpatient management of severe acute malnutrition (SAM) children is recommended. However, clinical assessment of such interventions is tedious in humanitarian settings and modalities evidence on the impact of psychosocial interventions alongside nutrition protocol for SAM is scarce. This survey aimed to gather expert opinions on the optimal design of a combined psychosocial and nutrition intervention feasible in humanitarian settings. Methods: From March to May 2018, an online survey was emailed to international experts in nutrition and mental health and psychosocial support, mainly from academia and international non-governmental organisations (INGOs). It included multiple choices questions on the key components of an optimal combined intervention. Results: Of the 76 experts targeted, 20 responded. 11 (55%) belonged to INGOs, 2 (10%) to academia, and 4 (20%) to international organizations and donors. For most respondents, a combined intervention should be provided in weekly 45-minuites counselling sessions, provided individually (rather than in a group) and at home (rather than at a health center). None of the proposed ideal duration (two, four or six months) gained the majority of votes. Experts thought that 35% staff training should be in “Active listening for psychosocial support”, and 30% in “Early child development”, 25% in “Maternal depression” and 9% in “Anthropometric measurements”. They estimated that a combined intervention could improve SAM recovery rate by 10% (min-max: 0-19%) vs. the nutritional protocol alone. Qualitative results highlighted the importance of tailoring the intervention to the individual, the population and the settings; as well as considering feasibility and scalability at the design stage. Conclusion: These findings could guide further research on the impact of psychosocial interventions on SAM children’s health and development, and help designing innovative approaches to treat undernutrition.
Modelling mode I failure at crack tip with verifications using digital image correlation
Authors: Kok Chee Kuang , Koh Eric Ren Wei , Chen Gooi Mee 
Published: October 2021

Background: Linear elastic fracture mechanics (LEFM) applies to sharp cracks, although crack sites such as holes and slots are often blunt cracks with nonzero widths. The advancement in finite element method (FEM) has enabled the calculation of stress intensity factor (SIF) for unstable crack growth prediction, regardless of crack shape and size, but the calculations often differ from contour to contour. Hence, the purpose of this work is to determine if SIFs computed using commercial FEM have experimentally verifiable advantages over the traditional stress-based modelling approaches in predicting Mode I brittle failure at blunt crack tip. Methods: Experiments and simulations were conducted on brittle Poly(methyl methacrylate) (PMMA) plastic to compare the actual and predicted strain fields and SIFs at crack tip, and the critical force at which unstable crack growth initiates. A centrally straight cracked Brazilian disc made of PMMA was subjected to purely Mode I fracture. Its strain fields were measured from deformed speckle patterns using digital image correlation software. The same disc was modelled using plane stress model in FEM. By applying the critical force, SIFs were then computed using ANSYS pre-meshed crack method at different contours away from the crack tip. The effects of element type, mesh size and crack width on the simulated SIFs were investigated. Results: It was found that the experimental critical load agreed well with LEFM prediction based on PMMA fracture toughness in published literature. Disc failure happened at the first sign of tensile yield at the crack tip in the finite element model with a triangle mesh. Digital image correlation clearly shows the occurrence of unstable crack growth at critical force. It also shows comparable far field strain responses to the FEM model. Conclusions: The computed SIFs were inconsistent, and their usefulness in predicting unstable crack growth requires further investigation.
MathOptInterface: A Data Structure for Mathematical Optimization Problems
Authors: Legat Benoît , Dowson Oscar , Garcia Joaquim Dias , Lubin Miles 
Published: October 2021

We introduce MathOptInterface, an abstract data structure for representing mathematical optimization problems based on combining predefined functions and sets. MathOptInterface is significantly more general than existing data structures in the literature, encompassing, for example, a spectrum of problems classes from integer programming with indicator constraints to bilinear semidefinite programming. We also outline an automated rewriting system between equivalent formulations of a constraint. MathOptInterface has been implemented in practice, forming the foundation of a recent rewrite of JuMP, an open-source algebraic modeling language in the Julia language. The regularity of the MathOptInterface representation leads naturally to a general file format for mathematical optimization we call MathOptFormat. In addition, the automated rewriting system provides modeling power to users while making it easy to connect new solvers to JuMP. Summary of Contribution: This paper describes a new abstract data structure for representing mathematical optimization models with a corresponding file format and automatic transformation system. The advances are useful for algebraic modeling languages, allowing practitioners to model problems more naturally and more generally than before.
The results of immunohistochemical study of antibodies to CYP11B2 in primary hyperaldosteronism
Authors: Romanova N. Yu. , Selivanova L. S. , Platonova N. M. , Beltsevich D. G. , Troshina E. A. 
Published: October 2021

Background: Aldosterone-producing adrenocortical adenoma (APA) is responsible for the majority of cases clinically diagnosed as primary aldosteronism. Aldosterone synthase (CYP11B2) is one of the enzymes that play essential roles in aldosterone synthesis and is involved in the pathogenesis of primary aldosteronism. Recent studies have demonstrated that various factors influence the expression and function of CYP11B2 in APA. In particular, somatic mutations, such as gain-of-function and loss-of-function mutations have been identified in several genes, each of which encodes a pivotal protein that affects the calcium signaling pathway, the expression of CYP11B2, and aldosterone production. On the other hand, CYP11B2 also catalyzes the conversion of cortisol to 18-hydroxycortisol and subsequently converts 18-hydroxycortisol to 18-oxocortisol. The article also discusses the clinical significance of 18-oxocortisol, an important biomarker for the diagnosis of primary aldosteronism. Somatic mutations in aldosterone-driver genes are strongly associated with CYP11B2 expression and have been only detected in the CYP11B2-positive tumor area, that indicating heterogeneous expression of CYP11B2 in tumor.Aim: to assess the functional heterogeneity of adrenal tumors in primary aldosteronismMaterials and methods: Retrospective evaluation adrenal tumors from patients with primary aldosteronism (n=20). According to CT unilateral macrohyperplasia was detected in 19 patients (95% of total), all of them were confirmed to have unilateral hyperproduction of aldosterone according to AVS. Selected tumors werestained with anty-CYP11B2 antibody. We evaluated the CYP11B2 expression in the adenoma and in the adjacent adrenal cortex.Results: Immunohistochemistry studies of the resected adrenals from 20 patients with PA operated due to unilateral production of aldosterone using CYP11B2 staining showed that 10 of those with an adenoma on CT scanning showed CYP11B2 staining in the adenoma. Furthermore, 5 cases of an unilateral adenoma, showed CYP11B2 staining in the adjacent adrenal cortex and an absence of staining for CYP11B2 in the adenoma. 5 cases showed CYP11B2 expression is heterogeneously immunolocalized throughout the tumor area.Conclusion: Thus, the functional heterogeneity of adrenal tumors in primary aldosteronism has been proven. It is necessary to compare the data of immunohistochemical studies on the expression of CYP11B2 with the indicators of the level of 18-hydroxycortisol, 18-oxocortisol.
Lymphatic system and adipose tissue: Crosstalk in health and disease
Authors: Klimontov V. V. , Bulumbaeva D. M. 
Published: October 2021

The lymphatic system (LS) is one of the main integrative systems of the body, providing protective and transport functions. In recent years, interactions between LS and adipose tissue (AT) have been of particular interest. Lymphatic vessels play an important role in metabolic and regulatory functions of AT, acting as a collector of lipolysis products and adipokines. In its turn, hormones and adipocytokines that produced in adipocytes (including leptin, adiponectin, IL-6, TNF-α, etc.) affect the function of lymphatic endothelial cells and control the growth of lymphatic vessels. Cooperation between LS and AT becomes pathogenetically and clinically important in lymphedema and obesity. It is known that both primary and secondary lymphedema are characterized by increased fat accumulation which is associated with the severity of lymphostasis and inflammation. Similarly, in obesity, the drainage function of LS is impaired, which is accompanied by perilymphatic mononuclear infiltration in the AT. The development of these changes is facilitated by endocrine dysfunction of adipocytes and impaired production of adipocytokines. The increase in the production of inflammatory mediators and the disruption of the traffic of inflammatory cells causes a further deterioration in the outflow of interstitial fluid and exacerbates the inflammation of the AT, thereby forming a vicious circle. The role of lymphangiogenesis in AT remodeling in obesity needs further research. Another promising area of research is the study of the role of intestinal LS in the development of obesity and related disorders. It has been shown that the transport of chylomicrons from the intestine depends on the expression of a number of molecular mediators (VEGF-C, DLL-4, neuropilin-1, VEGFR-1, CD36/FAT, etc.)in the endotheliocytes of the intestinal lymphatic vessels, as well as the functioning of «push-button» and “zippering” junctions between endothelial cells. New approach to the treatment of obesity based on blockade of lymphatic chylomicrontransport has been experimentally substantiated. Further identification of the molecular mechanisms and signaling pathways that determine the remodeling of AT in lymphedema and obesity are likely to provide new approaches to the treatment of these diseases.
Medicaid, Earnings, and Heterogeneous Treatment Effects: Evidence from the Oregon Health Insurance Experiment
Authors: Tidemann Krieg 
Published: October 2021

Abstract
The Medicaid and labor supply empirical literature offers competing conclusions of zero effects and significant reductions in earnings. However, zero effects are only theoretically consistent with the earnings distribution’s extremes. Medicaid participants with positive pre-treatment labor supply should unequivocally decrease earnings. This paper clarifies the literature’s ambiguity by combining quantile regression with data from the Oregon Health Insurance Experiment. The distributional impacts imply that zero effects are not universally representative of Medicaid households. The annual earnings impact of Medicaid participation ranges between increases of $1400 to deceases of $3120 for single adults. Pre-existing mental illness or health constraints on work account for counterintuitive positive earnings impacts. By demonstrating that sample compositional differences determine whether Medicaid’s labor supply impact is zero or negative, this paper offers a reconciliation to the range of existing estimates in the empirical literature.
Circularly polarized CPW fed MIMO/Diversity antenna for Wi-Fi and WLAN applications
Authors: Kumar Anubhav , De Asok , Jain Rakesh K. 
Published: October 2021

Abstract
In this paper, circular polarized two-element and eight-element CPW fed MIMO/Diversity antenna with defected ground is presented. The dimension of the two-port antenna is 30 mm × 30 mm whereas the edge-to-edge gap between radiating elements is 5.65 mm. The |S11| in dB varies from 4.95 to 5.95 GHz with a gain up to 4.1 dB and efficiency is more than 90%. The isolation of two-element CPW antenna is more than 20 dB with open and diagonal stub in the ground whereas more than 18.7 dB for eight-element antenna without decoupling network. The circular and rectangular stub perturbs the surface current in the ground and is responsible for RHCP and LHCP in two-elements and eight-elements antenna where it covers the complete operating band. The acceptable ECC, TARC, DG, and CCL values of the antenna are determined which represents the diversity characteristics of the antenna. The Wi-Fi/WLAN applications can be fulfilled with the proposed two and eight-element antenna.