bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bcl-2-associated transcription factor 1 Ser290 phosphorylation mediates DNA damage response and regulates radiosensitivity in gastric cancer
Authors: Liu Jia , Li Jingyi , Sun Zhao , Duan Yangmiao , Wang Fengqin , Wei Guangwei , Yang Jing-Hua 
Published: August 2021

AbstractBackgroundDNA damage response plays critical roles in tumor pathogenesis and radiotherapy resistance. Protein phosphorylation is a critical mechanism in regulation of DNA damage response; however, the key mediators for radiosensitivity in gastric cancer still needs further exploration.MethodsA quick label-free phosphoproteomics using high-resolution mass spectrometry and an open search approach was applied to paired tumor and adjacent tissues from five patients with gastric cancer. The dysregulated phosphoproteins were identified and their associated-pathways analyzed using Gene Set Enrichment Analysis (GSEA). The mostly regulated phosphoproteins and their potential functions were validated by the specific antibodies against the phosphorylation sites. Specific protein phosphorylation was further analyzed by functional and clinical approaches.Results832 gastric cancer-associated unique phosphorylated sites were identified, among which 25 were up- and 52 down-regulated. Markedly, the dysregulated phosphoproteins were primarily enriched in DNA-damage-response-associated pathways. Particularly, the phosphorylation of Bcl-2-associated transcription factor 1 (BCLAF1) at Ser290 was significantly upregulated in tumor. The upregulation of BCLAF1 Ser290 phosphorylation (pBCLAF1 (Ser290)) in tumor was confirmed by tissue microarray studies and further indicated in association with poor prognosis of gastric cancer patients. Eliminating the phosphorylation of BCLAF1 at Ser290 suppressed gastric cancer (GC) cell proliferation. Upregulation of pBCLAF1 (Ser290) was found in association with irradiation-induced γ-H2AX expression in the nucleus, leading to an increased DNA damage repair response, and a marked inhibition of irradiation-induced cancer cell apoptosis.ConclusionsThe phosphorylation of BCLAF1 at Ser290 is involved in the regulation of DNA damage response, indicating an important target for the resistance of radiotherapy.
Ginsenoside Rb1 induces a pro-neurogenic microglial phenotype via PPARγ activation in male mice exposed to chronic mild stress
Authors: Zhang Lijuan , Tang Minmin , Xie Xiaofang , Zhao Qiuying , Hu Nan , He Hui , Liu Gangcai , Huang Shiqi , Peng Cheng , Xiao Ying , You Zili 
Published: August 2021

Abstract
Background
Anti-inflammatory approaches are emerging as a new strategy for the treatment of depressive disorders. Ginsenoside Rb1 (GRb1), a major component of Panax ginseng, can inhibit inflammatory cascade and alleviate depressive-like behaviors. Microglia can promote or inhibit adult hippocampal neurogenesis according to their functional phenotypes. Here, we examine whether GRb1 may exert antidepressant effects by promoting a pro-neurogenic phenotype of microglia and thereby increasing neurogenesis.

Methods
The antidepressant effects of GRb1 or the licensed antidepressant imipramine (IMI) were assessed in chronic mild stress (CMS)-exposed male mice. The depressive-like behaviors of mice were evaluated by sucrose preference test, forced swimming test (FST), and tail suspension test (TST). The microglial phenotypes were identified by pro- and anti-inflammatory cytokine expression and morphological properties, analyzed by RT-qPCR, western blotting, and immunofluorescence staining. The effect of GRb1-treated microglia on adult hippocampal neurogenesis in vivo and in vitro was detected using immunofluorescence staining.

Results
Behavioral assessment indicated that GRb1 or IMI treatment alleviated depressive-like behaviors in CMS-exposed mice. Immunofluorescence examination demonstrated that GRb1 induced a pro-neurogenic phenotype of microglia via activating PPARγ in vivo and in vitro, which were effectively reversed by the PPARγ inhibitor GW9662. In addition, GRb1-treated microglia increased the proliferation and differentiation of neural precursor cells.

Conclusions
These findings demonstrated that GRb1 alleviated depressive-like behaviors of CMS-exposed male mice mainly through PPARγ-mediated microglial activation and improvement of adult hippocampus neurogenesis.
A critical cartography of domestic violence policies in Mozambique
Authors: Jethá Eunice , Keygnaert Ines , Seedat Mohamed , Nhampoca Joaquim , Sidat Mohsin , Roelens Kristien 
Published: August 2021

AbstractBackgroundDomestic violence (DV) affects millions of people worldwide, especially women impacting their health status and livelihoods. To prevent DV and to improve the quality of victims’ lives, Mozambican governmental and non-governmental entities are making efforts to develop adequate policies and legislation and to improve the accessibility of services for victims of DV.However, a critical review of whether or not current policies and legislation concerning DV in Mozambique are in agreement with international guidelines has yet to be examined. Therefore, this paper aims to map the Mozambican legislative and policy responses to DV. It also strives to analyse their alignment with international treaties and conventions and with each other.MethodsThrough a critical cartography, documents were selected and their content analysed. Some of these documents were not available online, printed versions were not available on the field and some were not up to date. Therefore, we had to search for them via physical office visits at governmental institutions with a responsibility to deal with DV aspects. These documents were listed and analysed for key content applying a framework inquiring on recommendations of international agencies such as World Health Organization. Subsequently, we compared these policies with international conventions and treaties of which Mozambique is signatory and with each other to identify discrepancies.ResultsOverall, six institutions were visited assuring identification of all available information and policy documents on DV. We identified a total of fifteen national DV documents of which five were on laws, one on policy and nine institutional strategic/action plans. Most of the national DV documents focused on strategies for assistance/care of victims and prevention of DV. Little focus was found on advocacy, monitoring and evaluation.ConclusionsMozambique has demonstrated its commitment by signing several international and regional treaties and conventions on DV. Despite this, the lack of consistency in the alignment of international treaties and conventions with national policies and laws is remarkable. However, a gap in the reliable translation of national policies and laws into strategic plans is to be found particularly in relation to naming type, beneficiaries, main strategies and multi-sectorial approach.
Virome analysis of ticks in a forest region of Liaoning, China: characterization of a novel hepe-like virus sequence
Authors: Yang Zijun , Zhang Ju , Yang Shixing , Wang Xiaochun , Shen Quan , Sun Guangming , Wang Hao , Zhang Wen 
Published: August 2021

Abstract
Background
Ticks (class Arachnida, subclass Acari) are vectors of transmitting a broad range of pathogenic microorganisms, protozoa, and viruses affecting humans and animals. Liaoning Province is rich in forests where different animals and, abundant Haemaphysalis longicornis ticks exist.

Methods
Using viral metagenomics, we analyzed the virome in 300 Haemaphysalis longicornis ticks collected from June to August 2015 in the forested region of Liaoning Province, China.

Results
From the 300 ticks, 1,218,388 high-quality reads were generated, of which 5643 (0.463%) reads showed significant sequence identity to known viruses. Sequence and phylogenetic analysis revealed that viral sequences showing a close relationship with Dabieshan tick virus, Aleutian mink disease virus, adeno-associated virus, Gokushovirus, avian gyrovirus 2 were present in the virome of these ticks. However, the significance of these viruses to human and animal health requires further investigation. Notably, an hepe-like virus, named tick-borne hepe-like virus sequence, was obtained and was highly prevalent in these ticks with a rate of 50%. Nevertheless, one constraint of our study was the limited geographical distribution of the sampled ticks.

Conclusion
Our study offers an overview of the virome in ticks from a forest region of Liaoning Province and provides further awareness of the viral diversity of ticks.
Gut micobiota alteration by Lactobacillus rhamnosus reduces pro-inflammatory cytokines and glucose level in the adult model of Zebrafish
Authors: Bootorabi Fatemeh , Saadat Farshid , Falak Reza , Manouchehri Hamed , Changizi Reza , Mohammadi Hasan , Safavifar Farnaz , Khorramizadeh Mohammad Reza 
Published: August 2021

Abstract
Objective
Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient’s circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology.

Results
In this trial 3-month old male adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After 5 days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p = 0.0001). These results suggest that probiotic intervention decreased the blood glucose level in the T2DM-P group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.
Re-staging and follow-up of rectal cancer patients with MR imaging when “Watch-and-Wait” is an option: a practical guide
Authors: Santiago Inês , Rodrigues Bernardete , Barata Maria , Figueiredo Nuno , Fernandez Laura , Galzerano Antonio , Parés Oriol , Matos Celso 
Published: August 2021

AbstractIn the past nearly 20 years, organ-sparing when no apparent viable tumour is present after neoadjuvant therapy has taken an increasingly relevant role in the therapeutic management of locally-advanced rectal cancer patients. The decision to include a patient or not in a “Watch-and-Wait” program relies mainly on endoscopic assessment by skilled surgeons, and MR imaging by experienced radiologists. Strict surveillance using the same modalities is required, given the chance of a local regrowth is of approximately 25–30%, almost always surgically salvageable if caught early. Local regrowths occur at the endoluminal aspect of the primary tumour bed in almost 90% of patients, but the rest are deep within it or outside the rectal wall, in which case detection relies solely on MR Imaging. In this educational review, we provide a practical guide for radiologists who are, or intend to be, involved in the re-staging and follow-up of rectal cancer patients in institutions with an established “Watch-and-Wait” program. First, we discuss patient preparation and MR imaging acquisition technique. Second, we focus on the re-staging MR imaging examination and review the imaging findings that allow us to assess response. Third, we focus on follow-up assessments of patients who defer surgery and confer about the early signs that may indicate a sustained/non-sustained complete response, a rectal/extra-rectal regrowth, and the particular prognosis of the “near-complete” responders. Finally, we discuss our proposed report template.
Improving workflow in prostate MRI: AI-based decision-making on biparametric or multiparametric MRI
Authors: Hötker Andreas M. , Da Mutten Raffaele , Tiessen Anja , Konukoglu Ender , Donati Olivio F. 
Published: August 2021

Abstract
Objectives
To develop and validate an artificial intelligence algorithm to decide on the necessity of dynamic contrast-enhanced sequences (DCE) in prostate MRI.

Methods
This study was approved by the institutional review board and requirement for study-specific informed consent was waived. A convolutional neural network (CNN) was developed on 300 prostate MRI examinations. Consensus of two expert readers on the necessity of DCE acted as reference standard. The CNN was validated in a separate cohort of 100 prostate MRI examinations from the same vendor and 31 examinations from a different vendor. Sensitivity/specificity were calculated using ROC curve analysis and results were compared to decisions made by a radiology technician.

Results
The CNN reached a sensitivity of 94.4% and specificity of 68.8% (AUC: 0.88) for the necessity of DCE, correctly assigning 44%/34% of patients to a biparametric/multiparametric protocol. In 2% of all patients, the CNN incorrectly decided on omitting DCE. With a technician reaching a sensitivity of 63.9% and specificity of 89.1%, the use of the CNN would allow for an increase in sensitivity of 30.5%. The CNN achieved an AUC of 0.73 in a set of examinations from a different vendor.

Conclusions
The CNN would have correctly assigned 78% of patients to a biparametric or multiparametric protocol, with only 2% of all patients requiring re-examination to add DCE sequences. Integrating this CNN in clinical routine could render the requirement for on-table monitoring obsolete by performing contrast-enhanced MRI only when needed.
Toward a systematic conflict resolution framework for ontologies
Authors: Keet C. Maria , Grütter Rolf 
Published: August 2021

Abstract
Background
The ontology authoring step in ontology development involves having to make choices about what subject domain knowledge to include. This may concern sorting out ontological differences and making choices between conflicting axioms due to limitations in the logic or the subject domain semantics. Examples are dealing with different foundational ontologies in ontology alignment and OWL 2 DL’s transitive object property versus a qualified cardinality constraint. Such conflicts have to be resolved somehow. However, only isolated and fragmented guidance for doing so is available, which therefore results in ad hoc decision-making that may not be the best choice or forgotten about later.

Results
This work aims to address this by taking steps towards a framework to deal with the various types of modeling conflicts through meaning negotiation and conflict resolution in a systematic way. It proposes an initial library of common conflicts, a conflict set, typical steps toward resolution, and the software availability and requirements needed for it. The approach was evaluated with an actual case of domain knowledge usage in the context of epizootic disease outbreak, being avian influenza, and running examples with COVID-19 ontologies.

Conclusions
The evaluation demonstrated the potential and feasibility of a conflict resolution framework for ontologies.
Characterisation of smallholders’ goat production systems in the Fatick area, Senegal
Authors: Sow Fafa , Camara Younouss , Traore El Hadji , Cabaraux Jean-François , Missohou Ayao , Antoine-Moussiaux Nicolas , Hornick Jean-Luc , Moula Nassim 
Published: August 2021

AbstractSenegal, like the other Sahelian countries, remains an important livestock area, particularly for ruminants, with almost 36% of its livestock population was goat in 2016. The national herd increased from 2010 to 2016, from 3.32 to 3.54 million head of cattle, 5.6 to 6.68 million sheep and 4.8 to 5.7 million goats, i.e. annual growth rates of 1.1%, 3.2% and 3.13% respectively. Thus, due to diversification of local agricultural resources and the strengthening of goat breeding techniques in the Fatick area, a programme aiming to develop the local goat sector was set up in 2010. The programme focused on improving animal husbandry, providing new added values to goat products and structuring the goat sector. This study’s aim is to better understand goat rearing systems in the area, in order to propose, together with the herders, ways of improving these systems, taking into account family and socio-cultural considerations. To better evaluate the diversity in different production systems, a survey of animal rearing practices was carried out. The survey involved 45 farmers in four localities. It revealed that the farmers were all agro-pastoralists practising a mixed farming system. The majority (93%) supplemented their animals with agricultural by-products, agro-forestry and kitchen leftovers. A multiple correspondence analysis identified three groups: cluster 1 (milk producers and processors into traditional curdled milk, selling animals for household needs), cluster 2 (milk processors into yoghurt and cheese) and cluster 3 (goat vendors in pastoral and the agro-pastoral system).The study of the objectives and contexts of goat farming in the Fatick will enable policy-makers to design strategies for the sustainable development of family goat farming in the area.
On two kinds of the reverse half-discrete Mulholland-type inequalities involving higher-order derivative function
Authors: Chen Qiang , Yang Bicheng 
Published: August 2021

AbstractBy means of the weight functions, Hermite–Hadamard’s inequality, and the techniques of real analysis, a new more accurate reverse half-discrete Mulholland-type inequality involving one higher-order derivative function is given. The equivalent statements of the best possible constant factor related to a few parameters, the equivalent forms, and several particular inequalities are provided. Another kind of the reverses is also considered.
Solvability for generalized nonlinear two dimensional functional integral equations via measure of noncompactness
Authors: Singh Soniya , Singh Bhupander , Nisar Kottakkaran Sooppy , Hyder Abd-Allah , Zakarya M. 
Published: August 2021

AbstractIn this article, we provide the existence result for functional integral equations by using Petryshyn’s fixed point theorem connecting the measure of noncompactness in a Banach space. The results enlarge the corresponding results of several authors. We present fascinating examples of equations.
The influence of fasting and carbohydrate-enriched drink administration on body water amount and distribution: a volunteer randomized study
Authors: Kukliński Jakub , Steckiewicz Karol P. , Sekuła Bartosz , Aszkiełowicz Aleksander , Owczuk Radosław 
Published: August 2021

Abstract
Background
Fasting prior to anesthesia is considered aspiration prophylaxis. However, prolonged food and drink restrictions may increase the risk of other complications. The aim of this study was to assess whether a carbohydrate-enriched drink (Nutricia™ preOp®), recommended by the enhanced recovery after surgery (ERAS) protocol, can improve body hydration in fasting healthy individuals.

Methods
Measurements were done with the bioelectric impedance analysis with a Fresenius body composition monitor. Body composition, total body water, water distribution, and hemodynamic parameters were measured at the beginning of the study and after 10 h and 12 h of fasting. Patients fasted for 10 h and then were divided into two groups: the control (n = 40) and the pre-op group (n = 41). The pre-op group received 400 mL of Nutricia™ preOp®, as suggested in the ERAS guidance. The two-tailed Student’s t test was used to compare two groups with normally distributed data and homogenous variances; if variances were heterogeneous, Welch’s test was used. The Mann-Whitney U test was used to compare two groups with non-normal data distribution. p < 0.05 was considered statistically significant.

Results
We found no significant differences between the control and pre-op groups regarding body water distribution and body composition. We did not observe significant losses in the total body water after fasting. Also, blood pressure was not affected by fasting.

Conclusion
We have proven that pre-op did not impact either body composition or body water.

Trial registration
ClinicalTrials.gov, NCT04665349. Registered on 11 December 2020—retrospectively registered.
Evidence of effect of riparian attributes on listed freshwater fishes and mussels and their aquatic critical habitat: a systematic map protocol
Authors: Caskenette Amanda , Durhack Travis , Hnytka Sarah , Kovachik Colin , Enders Eva 
Published: August 2021

AbstractBackgroundHabitat that is necessary for the survival and recovery of a species listed as threatened, endangered or extirpated (i.e., Critical Habitat) is protected in Canada. For freshwater aquatic species, Critical Habitat may include the riparian zone, however, it is unclear how much of this riparian habitat needs to be protected to support the survival and recovery of a listed species. The riparian zone mainly affects aquatic species through its indirect effect on aquatic habitat according to five main processes: erosion, filtration, infiltration, shading, and subsidization. To provide quantitative evidence to support the delineation of riparian Critical Habitat, a systematic map will be used to create a searchable database containing the current state of knowledge regarding the relationships between the riparian zone attributes (e.g., size, vegetation) and fishes and mussels, aquatic features, and riparian processes.MethodsWe will search for primary research articles in bibliographic databases and relevant organizational websites for primary literature, theses, preprints, and grey literature including reports, along with models using a search string specific to riparian habitat. The results will be screened at title and abstract, and full text levels against predefined inclusion criteria and consistency checking will be performed to ensure the inclusion criteria are consistent across multiple reviewers. Quantitative and qualitative data including study details and methods, the riparian habitat, and the waterbody and upland habitat use will be extracted. The findings of the systematic map will be provided in a manuscript and a searchable database accompanied by a decision tree to support biologists in providing scientifically defensible delineation of riparian Critical Habitat for aquatic species at risk in Canada.
A case report of primary pulmonary artery intimal sarcoma
Authors: Bai Xiaofang , Ruan Litao 
Published: August 2021

AbstractBackgroundPulmonary artery intimal sarcoma (PAS) is a very rare disease, its prevalence is about 0.001–0.003%. PAS is often misdiagnosed as acute or chronic pulmonary thromboembolism due to its clinical presentation and radiological findings. Thus, early diagnosis is very crucial and may improve patient outcome.Case presentationHere, we report a case in a Chinese male where the symptom presentation was episodes of shortness of breath. Transthoracic echocardiography showed a solid mass in the pulmonary valve orifice, which was demonstrated to be a pulmonary artery intimal sarcoma diagnosed by histopathology. In this case, the initial differential diagnosis included pulmonary embolism. Because the initial symptom of primary pulmonary artery sarcoma is extremely similar to the pulmonary embolism, half of them may be misdiagnosed as pulmonary embolism. Imaging studies are very helpful. Ultrasound and CT are the best due to their resolution and ability to assess the relationship of the mass with the surrounding structures. The final diagnosis is mostly made after surgical excision and this is the most effective treatment. At the same time, radiotherapy and chemotherapy after surgery is also an adjuvant treatment.ConclusionWe report a very rare case of pulmonary artery intimal sarcoma. Due to late diagnosis and delayed treatment in this case, the patient displayed a poor prognostic. Early diagnosis and right treatment can improve the prognosis of PAS and optimize overall health.