bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal Stem Cell Transplantation in Newly Diagnosed Type-1 Diabetes Patients: A Phase I/II Randomized Placebo Controlled Clinical Trial
Authors: Izadi Mahmoud , Nejad Anavasadat Sadr Hashemi , Moazenchi Maedeh , Masoumi Safdar , Rabbani Ali , Kompani Farzad , Asl Amir Abbas Hedayati , Abbasi Fatemeh , Jarooghi Neda , Meybodi Mohamad Ali Mohseni , Setoodeh Aria , Abbasi Farzaneh , Hosseini Seyedeh Esmat , Nia Fatemeh Moeini , Yazdi Reza Salman , Navabi Roghayeh , Hajizadeh-Saffar Ensiyeh , Baharvand Hossein 
Published: September 2021

Abstract
BackgroundType-1 diabetes (T1D) occurs following autoimmune-induced pancreatic beta cells death. Among several treatment modalities, Mesenchymal Stem Cells (MSCs) transplantation is promising for autoimmune disorders due to immunomodulation, regeneration, and migration to damaged tissue upon systemic injection. This study assessed the safety and efficacy of intravenous injection of autologous bone marrow-derived MSCs in newly diagnosed T1D patients. MethodsAfter receiving informed consent, 21 patients who met the study criteria were enrolled and randomly assigned to receive either MSCs or placebo. Each patient in the experimental group received two doses of MSCs and followed for at least one year post-transplantation. ResultsThe results have shown that this transplantation is safe and significantly reduces the number of hypoglycemic episodes. MSCs transplantation improved glycated hemoglobin (HbA1c), shifted serum cytokine patterns from pro-inflammatory to anti-inflammatory, increased the number of regulatory T-cells in the peripheral blood, and improved quality of life. Early transplantation of MSCs significantly improved HbA1c and C-peptide levels and shifted pro-inflammatory cytokines to anti-inflammatory cytokines. Also, exercise combined with MSCs transplantation improved glycemic and immunologic indices. ConclusionsIn conclusion, autologous MSC transplantation is safe and effective, and its early transplantation is a promising treatment in newly-diagnosed T1D children suffering from hypoglycemic episodes. Trial registrationThis clinical trial was registered at the Iranian Registry of Clinical Trials (IRCT) with the identifier IRCT ID: IRCT2016070428786N1 registered on August 20, 2016 (Retrospectively registered) (https://en.irct.ir/trial/23256) and at the U.S. National Institutes of Health (ClinicalTrials.gov) with the related identifier NCT04078308 registered on September 6, 2019 (Retrospectively registered). (https://clinicaltrials.gov/ct2/show/NCT04078308)
HIF-1α stabilization modified by glutaredoxin-1 is critical for intestinal angiogenesis in NEC pathogenesis
Authors: Zhang Yunfei , Zhang Xiao , Tian Bing , Deng Qin , Guo Chunbao 
Published: September 2021

Abstract
Background: Hypoxia inducible factor (HIF-1α) are essential in the pathogenesis of necrotizing enterocolitis (NEC), which is stabilized by Grx1 deletion. Until now, the mechanism of HIF-1α in the intestinal microcirculation in NEC is not well defined. We intend to investigate the role of HIF-1α in the development of NEC in regulating the microcirculation and the following vasodilatory signal, VEGF. Materials and methods: Experimental NEC was induced in full-term C57BL/6 mouse and Grx1-/-pups through the formula gavage and hypoxia technique. The HIF-1α signal was blocked utilizing the HIF-1α inhibitor, YC-1. Intestinal tissues were collected at predetermined time points for the assessment of intestinal microcirculation and the HIF-1α activity involved signal. Results: We found that NEC inducement impaired the intestinal microcirculation, but intestinal blood flow and capillary density were ameliorated in Grx1-/-mice, which was associated with the GSH-protein adducts of HIF-1α in the intestinal tissue. Grx1 ablation could also promote vascular endothelial growth factor (VEGFA) production in the intestinal tissue. This intestinal microvascular improvement was not found in the HIF-1α inhibited mice, suggesting the HIF-1α dependent manner for intestinal microcirculatory perfusion. Conclusion: The current data demonstrated that HIF-1α signaling is involved in the intestinal microvascular modification during the pathogenesis of NEC, suggesting that targeting with HIF-1α might be a promising strategy for NEC treatment.
Research on the Effect of Texture on Wear Performance of High-Pressure Piston Pump Valve Pair
Authors: He Hengsuo , Wu Huaichao , Zhao Limei , Yang Lv , Yuan Kui , He Muyang 
Published: September 2021

Abstract
In this paper, the surface wear performance of the valve pair of a high-pressure axial piston pump is improved. Firstly, the friction and wear tests are carried out for different materials of the axial piston pump valve pair. Moreover, the friction and wear behavior of the valve pair contact is studied. A valve pair material that is more suitable for processing surface texture is chosen. Friction and wear models of a valve pair based on degradation coefficient are proposed and wear conditions of different textures are calculated. Lastly, the texture with the best anti-wear effect is selected. Finally, the selected textures are experimentally validated by laser processing. The results show that a long strip with a diameter of 400 μm, a depth of 20 μm, and an area ratio of 2% can effectively reduce wear. Through comparative analysis, it is concluded that the texture shape has the least influence on the wear of the valve pair. On the other hand, the area ratio of the texture has the greatest effect on the wear of the valve pair. As the diameter of the texture increases, the wear of the valve pair gradually decreases. As the texture depth increases, the wear amount of the valve pair shows a downward trend.
Silicon nitride ring resonator biosensors operated at 1310 nm wavelengths via TM-mode grating couplers
Authors: Castelló-Pedrero Lucía , Gómez-Gómez María I. , García-Rupérez Jaime , Griol Amadeu , Martínez Alejandro 
Published: September 2021

Abstract
In this work, we demonstrate a full photonic integrated device for performing highly-sensitive biosensing using silicon nitride ring resonators propagating transverse-magnetic modes at wavelengths around 1310 nm. The device includes fully-etched grating couplers as interfaces with external optical fibres. Sensing experiments are performed in a microfluidic environment using bovine serum albumin and anti-bovine serum albumin as biological agents. In comparison with other photonic platforms based on ring resonators propagating transverse-electric modes, our device shows a better detection performance though the efficiency of the grating couplers can be further improved.
Colorectal Cancer Prevalence and Survival in Castilla La Mancha (Spain). A Pilot Study
Authors: González Laura Valiente , Ibáñez Ricardo de Miguel , Sotos Francisco Escribano 
Published: September 2021

Abstract
Background: Colorectal cancer is the most commonly diagnosed cancer type and the second cause of cancer death in Spain. The primary risk factor for colorectal cancer is age, with 90% of all diagnosed patients aged over 50 years. Prognosis mainly depends on tumour stage. Aim: Conduct a pilot Colorectal Cancer prevalence and survival study in Cuenca (Spain) since there are almost no studies based on small populations. This is the first pilot study about survival in screening of colorectal cancer carried out in hospitals in Castilla La Mancha. Methods and Results: Retrospective descriptive cohort study was performed to include patients with colorectal cancer diagnosed by colonoscopy between May 2015 and April 2016, and who were followed up for 48 months. The study considered sociodemographic and clinical data of the patients. Survival curves were estimated using the Kaplan-Meier method. The proportional hazard rate associated with age, gender, stage and presence of metastasis was calculated using the Cox regression method. 57 patients were included in the study. The mean follow-up was 45.5 months. Ten patients died during the study, in seven cases the cause was colorectal cancer. The percentage of patients alive at 48-month follow-up were 82.4%. Conclusion: Colon cancer is a high-prevalence pathology, with adenocarcinoma being the most common histology. The results seem to indicate that it affects men more frequently, mortality rises with tumour stage at diagnosis and declines with use of chemotherapy. We present a pilot study that could justify large-scale epidemiological studies for the regional surveillance and evolution of colorectal cancer in Spain.
Association Between Levels of Urine Di-(2-ethylhexyl)phthalate Exposure, a Potentially Harmful and Invisible Chemical, and Heart Rate Variability in Young Adults
Authors: Chen Ching-Way , Hwang Jin-Shiang , Hsu Cheng-Chih , Lin Chien-Yu , Su Ta-Chen 
Published: September 2021

Abstract
BackgroundPhthalate exposure is associated with cardiovascular risk. Among the various phthalates, di-(2-ethylhexyl) phthalate (DEHP) is the most important plasticizer in our daily lives. This study investigated the association between DEHP exposure and the alteration of heart rate variability (HRV).MethodsDuring 2017-2019, we recruited 974 young adults to investigate the effects of living environments and dietary habits on cardiometabolic disorders in Taiwan. We quantitatively analyzed urinary metabolites of phthalates, including mono-(2-ethylhexyl) phthalate (MEHP), mono-(ethyl-5-hydroxyhexyl) phthalate (MEHHP), and mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP). A continuous electrocardiogram was recorded to obtain a 5-minute ECG. Time-domain and frequency-domain HRV analyses were performed.ResultsMultiple linear regression showed that urinary oxidized DEHP metabolites MEHHP and MEOHP were associated with decreased HRV after controlling for associated cardiovascular risk factors. A higher MEHHP level was associated with a lower TINN (triangular interpolation of NN interval histogram), very-low-frequency (VLF), and low frequency/high frequency (LF/HF) ratio. A higher MEOHP level was associated with a decreased LF/HF ratio. In addition, trend analysis showed that higher MEHHP and MEOHP quantiles were significantly associated with a decreased LF/HF ratio.Conclusions﻿DEHP is a potentially harmful and invisible chemicals. The urinary DEHP metabolites MEHHP and MEOHP are associated with decreased HRV, indicating an unfavorable autonomic balance in young adults in Taiwan.
Plasma fibrinogen as a predictor of residual renal function in patients undergoing peritoneal dialysis
Authors: Qi Ling , Huang Peipei , Wang Xiaoping , Zhu Shan , Wang Xiaomei , Su Zhen 
Published: September 2021

Abstract
Background： Inflammatory markers are powerful predictors of prognosis in patients undergoing peritoneal dialysis (PD). Plasma fibrinogen (FIB), an inflammatory marker, is considered an independent predictor of cardiovascular events and mortality in PD patients. However, little is known about the association between FIB and residual renal function (RRF) in PD patients. Material/Methods: We reviewed the clinical data of patients who started PD between January 2012 and December 2020 at the First Affiliated Hospital of Wenzhou Medical University. This study covered 472 patients in total. Residual renal function (RRF) was determined by a 24-hour urine collection and calculated from the average of creatinine and urea clearance. Clinical and biochemical data at the initiation of peritoneal dialysis was collected as baseline data. Results: The median follow-up duration was 31.55 (20.43-49.00) months. The RRF reduction rate was significantly greater in patients in the high-fibrinogen group (fibrinogen >4.0 g/L) compared with those in the control group (fibrinogen ≤4.0 g/L; 0.13 ± 0.15 mL/min/month/1.73m2 vs. 0.088 ± 0.10 mL/min/month/1.73m2; p<0.001). Using multiple linear regression analysis and adjusting for other risk factors, high FIB was an independent predictor of rapid RRF decline (P=0.033). The Cox proportional hazard model and the competing risk model revealed that an elevated plasma FIB level (HR=1.219, 95%CI 1.058-1.404; SHR=1.154, 95%CI 1.012-1.317) was independent factors for the progression to anuria. Conclusions: We demonstrated that a higher plasma fibrinogen level was significantly associated with a higher rate of RRF decline, and hyperfibrinogenemia was an independent risk factor for anuria in PD patients.
Neuroprotective Effects of Alisol A 24-acetate on Cerebral Ischemia-reperfusion Injury by Regulating PI3K/AKT Pathway
Authors: Lu Taotao , Li Huihong , Zhou Yangjie , Wei Wei , Ding Linlin , Zhan Zengtu , Liu Weilin , Tao Jing , Xue Xiehua 
Published: September 2021

Abstract
BackgroundNeuroinflammation and apoptosis are involved in the pathogenesis of ischemic stroke. Alisol A 24-acetate (24A) has a strong inhibitory effect on inflammation and cell apoptosis. The neuroprotective effect of 24A in the global cerebral ischemia/ reperfusion (GCI/R) is still unclear. Methods GCI/R mice was used to investigated the neuroprotective effect of 24A. Modified neurological deficit scores, Morris Water Maze and object recognition test were used to evaluate behaviors. The metabolism in brain regions was detected by MRS. The changes of microglia, astrocytes and neurons was detected. The inflammation and apoptosis were measured.Results The results showed that 24A improved behavioral dysfunction and brain metabolism, alleviate neuroinflammation and apoptosis, inhibited microglia and astrocytes activation, which is associated with the activation of PI3K/AKT pathway. ConclusionsTaken together, our study demonstrated that 24A could alleviate GCI/R injury through anti-neuroinflammation and anti-apoptosis via regulating the PI3K/AKT pathway.
Risk of Hepatitis B Virus Reactivation in HBsAg-negative, Anti-HBc-negative Patients Receiving Rituximab for Autoimmune Diseases in Hepatitis B Virus Endemic Areas
Authors: Lan Ting-Yuan , Lin Yen-Chun , Tseng Tai-Chung , Yang Hung-Chih , Kao Jui-Hung , Cheng Chiao-Feng , Lee Tai-Ju , Huang Shang-Chin , Lu Cheng-Hsun , Li Ko-Jen , Hsieh Song-Chou 
Published: September 2021

Abstract
Background

Rituximab is known to be associated with a high resolved hepatitis B virus (HBV) reactivation rate in patients with resolved HBV infection and hematologic malignancy. However, data regarding HBV reactivation in rheumatic patients receiving rituximab is limited.
Aims

To assess the HBV reactivation in HBsAg-negative patients receiving rituximab for autoimmune diseases in a large real-world cohort.
Methods

From March 2006 to December 2019, 900 patients with negative HBsAg receiving at least one cycle of rituximab for autoimmune diseases in a tertiary medical center in Taiwan were retrospectively reviewed. Clinical outcomes and factors associated with HBV reactivation were analyzed.
Results

After a median follow-up period of 3.3 years, 21 patients developed HBV reactivation, among which 17 patients were positive for anti-HBc and 4 were negative. Thirteen patients had clinical hepatitis flare, while 8 patients had HBsAg seroreversion without hepatitis. Old age, anti-HBc positivity, and undetectable serum anti-HBs level at rituximab initiation were associated with HBV reactivation. There was no significant difference in the HBV reactivation risk between rheumatoid arthritis and other autoimmune diseases. Among anti-HBc-negative patients, subjects without HBV vaccination at birth had an increased risk of HBV reactivation (4/368, 1.1%) compared with those who received vaccination (0/126, 0%).
Conclusions

In HBV endemic areas, patients with negative anti-HBc, especially those without vaccination at birth, were still at risk for HBV reactivation after receiving rituximab. Regular monitoring of HBV serology should be considered for all HBsAg-negative patients receiving rituximab for autoimmune diseases.
Bodies moving with sound: Effects of pitch and musical sounds on body-representations
Authors: Ley-Flores Judith , Alshami Eslam , Singh Aneesha , Bevilacqua Frédéric , Bianchi-Berthouze Nadia , Deroy Ophelia , Tajadura-Jiménez Ana 
Published: September 2021

Abstract
The effects of music on bodily movement and feelings, such as when people are dancing or engaged in physical activity, are well-documented - people may move in response to the sound cues, feel powerful, less tired. How sounds and bodily movements relate to create such effects? Here we deconstruct the problem and investigate how different auditory features affect people’s body-representation and feelings even when paired with the same movement. In three experiments, participants executed a simple arm raise synchronised with changing pitch in simple tones (Experiment 1), rich musical sounds (Experiment 2) and different absolute frequency ranges (Experiment 3), while we recorded indirect and direct measures on their movement, body-representations and feelings. Changes in pitch influenced people’s general emotional state as well as the various bodily dimensions investigated – movement, proprioceptive awareness and feelings about one’s body and movement. Adding harmonic content amplified the differences between ascending and descending sounds, while shifting the absolute frequency range had a general effect on movement amplitude, bodily feelings and emotional state. These results provide new insights in the role of auditory and musical features in dance and exercise, and have implications for the design of sound-based applications supporting movement expression, physical activity, or rehabilitation.
Exploring risky practices, Opioid Substitute Therapy (OST) and disease transmission among prison inmates in India - A cross-sectional study
Authors: Adhikary Anoshmita , Hossain Shaikh Shah , Narayanan Prakash 
Published: September 2021

Abstract
Background: Prison, a setting where high-risk persons including substance-use offenders are being convicted. They sometimes continue their risky behaviours which pose a risk to themselves, other prisoners and to the community. A cross-sectional study was conducted in ten correctional homes (CHs) in West Bengal to identify the substance use, sexual and hygiene practices among prison inmates inside correctional homes. Methods: In-depth interviews were conducted with 15 key informants posted inside prisons using an interview guide. Data were analysed using MS Excel 2019. Chi-Square tests were performed to ascertain the associations between variables.Results: This study confirmed the availability and use of substances within prisons, and homosexual as well as drug-injecting practices of inmates. According to key informants, many CH inmates have had infections such as contact dermatitis, scabies, UTIs, STIs, tuberculosis, HIV, and hepatitis B and C. Limited preventive and curative services such as healthy diet, isolation and treatment for HIV and TB were provided to infected inmates. The treatment completion rate in inmates who availed OST at CHs was 96.53%, while the re-addiction rate after released from prison was 98.07%. The routine screening for STIs and hepatitis, distribution of condom and needles, and treatment for hepatitis were absent in most CHs. The CHs lack mechanisms to ensure continuous drug supply, regular follow-up and treatment completion after incarceration, partner notification, hepatitis treatment, and health promotions.Conclusions: The improvements in infrastructure and uninterrupted health care delivery system can improve health outcomes of affected inmates and break the transmission to other inmates and general community.
Multiplex Immunohistochemistry Applies to Examination of PD-1/PD-L1 on Immune Cells for Prognosis Prediction in Non-Small Cell Lung Cancer
Authors: Chen Huiyong , Liao Hongliang , Gong Longlong , Liu Jingting , Zhou Lin , Kang Lin , Zheng Hongbo , Wan Renping 
Published: September 2021

Abstract
Background: Tumor cells expressing programmed cell death 1 (PD-1) and programmed cell death ligand 1 (PD-L1) correlate with a better prognosis of immunotherapy in non-small cell lung cancer (NSCLC) patients. Expression of PD-1 and PD-L1 on immune cells is also concerned by more and more researchers.Methods: This study included 174 patients with NSCLC, and collected from the month of December in 2012 to April 2019. Formalin-fixed paraffin-embedded (FFPE) samples from NSCLC patients were performed by multiplex immunohistochemistry (IHC) staining using CD8, CD57, CD68, CD163, PD-1 and PD-L1. Marker localization included each type of immune cell subset with PD-1 or PD-L1 was quantified and analyzed.Results: The present study revealed distribution characteristics of PD-1 and PD-L1 on CD8+ T cells, CD57+ NK cells, CD68+ macrophages and CD163+ M2 macrophages in NSCLC patients using multiplex IHC, which indicated that expression of PD-1 was higher on CD8+ T cells and expression of PD-L1 was higher on CD8+ T cells and CD68+ macrophages. Immune clustering analysis showed that the low immune feature group displayed more survival rate than the high group. The reason was due to higher ratios of CD8/PD-L1 in the low group compared with the high group in the NSCLC cohort. Further, the Kaplan-Meier analysis of survival rate according infiltration of different immune cells also indicated that low CD57+ NK cells and low CD68+ macrophages were associated with a higher survival rate. The similar results were observed in the Kaplan-Meier analysis of expression of PD-1 and PD-L1 on immune cells.Conclusions: Taken together, we displayed the expression characteristics of PD-1 and PD-L1 on tumor-infiltrating immune cells and revealed that high expression of PD-1 and PD-L1 on immune cells was associated with poor survival rate. The present study provided further evidence to better guide clinical treatment in NSCLC.
Source Tracking of Chicken Infectious Anemia Virus in a SPF Chicken Population, the Latest Report in China
Authors: Li Yan , Wang Jinjin , Zhang Yawen , Wang Yixin , Zhao Hui , Miao Xinyuan , Chi Zengna , Wang Qun , Cui Zhizhong , Chang Shuang , Zhao Peng 
Published: September 2021

Abstract
BackgroundLive attenuated vaccines have been extensively used to prevent infectious disease in poultry flocks. However, exogenous virus contamination in attenuated vaccines had been reported several times in the past, which brought enormous threat to poultry production and diseases prevention and control. Recently, an attenuated vaccine against Newcastle Disease produced in China was detected contamination with chicken infectious anemia virus (CIAV) in a routine inspection for exogenous virus. To understand the multiple routes of transmission of CIAV, and to better formulate correct prevention and control, it is necessary to find out the possible source of this contamination.Methodslood samples of SPF chickens that generated vaccines were collected to investigate CIAV antibody titers by ELISA test. Then, 14- to 18-day-old SPF chicken embryos (n=40) were randomly selected, DNA was extracted and detected by quantitative real-time PCR and nucleic acid dot hybridization assays. To further investigate the molecular features of the CIAV isolate, the complete genome of CIAV was amplified and analyzed.ResultsThe results showed both SPF chickens and embryos for vaccine preparation were CIAV-positive. In addition, the full-length genome sequences of CIAV from vaccines and SPF chicken embryos were consisted of 2,298 nucleotides (nt) with 100% homology, named as SDSPF2020 (Genbank accession number: MW660821). It demonstrated 95.7%-99.6% homology with the complete nucleotide sequences of reference strains, and shared the closest evolution relationship with the Chinese strain HLJ15125. Conclusion﻿This study illustrated that vertical transmission of CIAV from SPF chickens and embryos was an important way for exogenous virus contamination in vaccine production. As such, vaccine quality monitoring and health control are significant in the poultry industry from an environmental safety point of view.