bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual-color Fluorescence Cross-Correlation Spectroscopy to study Protein-Protein Interaction and Protein Dynamics in Live Cells
Authors: Hemmen Katherina , Choudhury Susobhan , Friedrich Mike , Balkenhol Johannes , Knote Felix , Lohse Martin , Heinze Katrin G. 
Published: September 2021

AbstractWe present a protocol and workflow to perform live cell dual-color fluorescence crosscorrelation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques. In dual-color FCCS, where the fluctuations in fluorescence intensity represents the dynamical “fingerprint” of the respective fluorescent biomolecule, we can probe co-diffusion or binding of the receptors. FRET, with its high sensitivity to molecular distances, serves as a well-known “nanoruler” to monitor intramolecular changes. Taken together, conformational changes and key parameters such as local receptor concentrations, and mobility constants become accessible in cellular settings.Quantitative fluorescence approaches are challenging in cells due to high noise levels and the vulnerable sample itself. We will show how to perform the experiments including the calibration steps. We use dual-color labeled β2-adrenergic receptor (β2AR) labeled (eGFP and SNAPtag-TAMRA). We will guide you step-by-step through the data analysis procedure using open-source software and provide templates that are easy to customize.Our guideline enables researchers to unravel molecular interactions of biomolecules in live cells in situ with high reliability despite the limited signal-to-noise levels in live cell experiments. The operational window of FRET and particularly FCCS at low concentrations allows quantitative analysis near-physiological conditions.Link to accompanying video: https://tr240.uni-wuerzburg.de/vippclass/index.php/s/TL8aWmwE9RjGfLE
Condition Medium of Glioblastoma Cell Lines Decreases the Viability of Glioblastoma Cells by Modulating Gene Expression Profile
Authors: Yavuz Mervenur , Akgül Sıddıka , Kaya Egemen , Demircan Turan 
Published: September 2021

AbstractGrade IV neoplasm of the central nervous system, GBM, is associated with poor prognosis and relatively short overall survival. Due to the current limitations in treatment methods, GBM is characterized as an incurable disease, and research to advance therapeutic options is required. Conditioned medium is commonly used inin-vitrostudies complementary to animal experiments to simulate tumor microenvironment and has the potential to challenge and expand our current understanding of secretome effect on tumor characteristics. This study aimed to investigate the effects of conditioned mediums of GBM cell lines on each other. Conditioned mediums’ cellular and molecular effects were evaluated using commonly employed techniques such as MTT assay, colony formation assay, wound healing assay, EdU labeling-based flow cytometry, and qRT-PCR. Our study demonstrated that conditioned medium harvested from U87 or LN229 cells at 48thh exhibited an anti-growth activity on each other by changing the gene expression pattern. Furthermore, the conditioned medium of LN229 decreased the migration capacity of U87 cells, and the conditioned medium of U87 cells significantly suppressed the LN229 proliferation. We believe that this initial work provides new insights for a better understanding of GBM cell lines’ secretome roles and highlights the necessity of further studies to unveil the secretome content.HighlightsConditioned medium harvested from GBM cells at different time points displayed various effects.Conditioned medium of GBM cell lines harvested at 48thh decreased the viability of each other.The expression level of anti-and pro-proliferative genes is altered upon condition medium treatment.
Mobile element integration reveals a chromosome dimer resolution system in Legionellales
Authors: Nicholson Beth , Deecker Shayna R. , Ensminger Alexander W. 
Published: September 2021

ABSTRACTIn bacteria, the mechanisms used to repair DNA lesions during genome replication include homologous recombination between sister chromosomes. This can lead to the formation of chromosome dimers if an odd number of crossover events occurs. The concatenated DNA must be resolved before cell separation to ensure genomic stability and cell viability. The broadly conserved dif/Xer system counteracts the formation of dimers by catalyzing one additional crossover event immediately prior to cell separation. While dif/Xer systems have been characterized or predicted in the vast majority of proteobacteria, no homologs to dif or xer have been identified in the order Legionellales. Here we report the discovery of a distinct single-recombinase dif/Xer system in the intracellular pathogen Legionella pneumophila. The dif site was uncovered by our analysis of Legionella mobile element-1 (LME-1), which harbors a dif site-mimic and integrates into the L. pneumophila genome via site-specific recombination. We demonstrate that lpg1867 (herein named xerL) encodes a tyrosine recombinase that is necessary and sufficient for catalyzing recombination at the dif site, and that deletion of dif or xerL causes filamentation along with extracellular and intracellular growth defects. We show that the dif/XerL system is present throughout Legionellales and that Coxiella burnetii XerL and its cognate dif site can functionally substitute for the native system in L. pneumophila. Lastly, we describe an unexpected link between C. burnetii dif/Xer and the maintenance of its virulence plasmids.Significance StatementThe maintenance of circular chromosomes depends on the ability to resolve aberrant chromosome dimers as they form. In most proteobacteria, broadly conserved Xer recombinases catalyze single crossovers at short, species-specific dif sites located near the replication terminus. Chromosomal dimerization leads to the formation of two copies of dif, leading to rapid site-specific recombination and elimination of the duplicated intervening sequence. The apparent absence of chromosome-dimer resolution mechanisms in Legionellales has been a mystery to date. By studying a phage-like mobile genetic element, LME-1, we have identified a previously unknown single-recombinase dif/Xer system that is not only widespread across Legionellales but whose activity is linked to virulence in two important human pathogens.
Will game-based learning enhance performance?
Authors: Chan Siew H. , Song Qian , Trongmateerut Pailin , Rivera Laurie H. 
Published: September 2021

Purpose
Extending the study of Chan et al. (2016), this paper aims to focus on specific aspects of performance (conceptual and factual knowledge) to provide insight into whether computer game attributes designed into Prrinciples Aren’t That Hard (PATH) improve performance.

Design/methodology/approach
A between-subjects experiment is conducted to test the hypotheses. The experimental and control groups are PATH and traditional paper medium, respectively.

Findings
The results reveal that PATH users perform better on the conceptual knowledge questions compared to the traditional paper medium users. No significant difference in performance on the factual knowledge (computational) questions is found between PATH and traditional paper medium users.

Research limitations/implications
This study demonstrates that PATH creates an engaging learning environment, which facilitates the acquisition of conceptual knowledge and improved (conceptual) performance. Research can investigate whether technology may be used to facilitate automation of computational tasks which downplay the importance of computational skills (factual knowledge) and focus on the design of computer game attributes in educational or training programs to enhance conceptual knowledge and (conceptual) performance.

Practical implications
The findings of this study will assist educators and educational technology developers to identify and design motivation-enhancing computer game features to promote remember and understand cognitive processes which improve (conceptual) performance.

Originality/value
Game-based learning serves as the underlying theoretical framework for the design of PATH used in an experimental study to examine the positive effects of motivation-enhancing computer game attributes on remember and understand cognitive processes which facilitate (conceptual) performance. This study also uses separate measures of performance; that is, conceptual and factual knowledge, to provide additional insight into the findings of Chan et al. (2016).
Financial performance assessment of branded and non-branded hotel companies. Analysis of the Portuguese case
Authors: Martins Catarina , Vaz Clara Bento , Alves Jorge Manuel Afonso 
Published: September 2021

PurposePortugal has been experiencing a continuous growth in tourism activity, with hospitality industry as one of the main tourism sectors. Therefore, the assessment of hotel companies’ performance is very important to assist decision processes. The purpose of this paper is to assess the financial performance (FP) of 570 hotel companies operating hotel units in Portugal in 2017. To explore the question of brand affiliation, a comparison was made between hotel companies with similar stars rating and market orientation. In addition, this paper intends to fill a gap in literature studying the Portuguese reality on the subject of brand affiliation.Design/methodology/approachThe present study uses a methodology based on data envelopment analysis (DEA) to assess the overall performance for each company, which further decomposed into the within-group performance and the technological gap. The performance of the hotel company is assessed through the aggregation of multiple financial indicators using the composite indicator (CI) derived from the DEA model. A bivariate analysis based on the Tobit regression to test the robustness of brand effect on FP of hotel companies (HC) was also included.FindingsThe empirical results show that branded companies, on average, have significantly better overall FP than non-branded companies. On the one hand, the brand effect tends to improve the within-group FP of HCs and the brand presents a statistically significant positive effect on the FP. On the other hand, the best practices are observed in both branded and non-branded companies.Practical implicationsThe results of this study illustrate that, globally, the better FP of the branded companies is because of their individual relative companies’ performance and a better model of operation given by the brand effect. Brand affiliation will generally allow for a better FP and essentially a better profitability for invested equity, a higher return on sales and a higher value added per employee.Originality/valueThe study provides important theoretical and practical contributions that can assist the strategic decision of the HCs in choosing to operate independently or to adopt brand affiliation. Also, it is innovative because the FP of branded and non-branded HCs is measured not using a set of individual financial ratios but through a single CI that aggregates those financial ratios, using a DEA model.
Do you hear my accent? How nonnative English speakers experience conflictual conversations in the workplace
Authors: Kim Regina , Ramirez-Marin Jimena Y. , Tasa Kevin 
Published: September 2021

Purpose
The purpose of this paper is to investigate the experiences of nonnative speakers in conflictual situations with native speakers in the workplace. In three studies, the authors examine whether nonnative speakers experience stereotype threat in workplace conflict situations with native speakers, whether stereotype threat is associated with certain conflict managing behaviors (e.g. yielding and avoiding) and the relationship between stereotype threat, satisfaction with conflict outcomes and processes, and objective conflict outcomes.

Design/methodology/approach
Studies 1 and 2 use critical incident recall methodology to examine nonnative speakers’ conflict behaviors and satisfaction with conflict outcomes. In Study 3, data were collected from a face-to-face simulation with a random-assignment design.

Findings
Findings suggest that nonnative speakers indeed experience heightened stereotype threat when interacting with native speakers in conflict situations and the experience of stereotype threat leads to less satisfaction with conflict outcomes, perceptions of goal attainment, as well as worse objective conflict outcomes.

Originality/value
The current study is one of the first studies to document the effects of accent stereotype threat on conflict behaviors and outcomes. More broadly, it contributes to the conflict studies literature by offering new insight into the effects and implications of stereotype threat on workplace conflict behaviors and outcomes.
Effects of trading partner relationships and knowledge complementarity on innovation performance
Authors: Astuti Endang Siti , Arifin Zainul , Wilopo Wilopo , Iqbal Mohammad 
Published: September 2021

PurposeThe purpose of this paper is to examine the effect of environmental characteristics, business partnership relationship, knowledge complementarity, organizational characteristics on knowledge management practices and innovation performance.Design/methodology/approachThe research used primary data, i.e. data obtained directly from the distribution of questionnaires to respondents, namely, the company’s managers/leaders/directors in the companies that became the research sample. Data were collected by sending questionnaires either by mail or email to the respondents. To examine the effect of environmental characteristics, business partnership relationship, knowledge complementarity and organizational characteristics on knowledge management practices and innovation performance.FindingsEnvironmental characteristics, business partnership relationship, knowledge complementarity and organizational characteristics partially have a significant effect on knowledge management practices. Environmental characteristics and business partnership relationship partially have a significant effect on innovation performance, while knowledge complementarity and organizational characteristics have no significant effect. For other constructs, knowledge management practices are able to be a moderator variable, which can relate these constructs with innovation performance.Originality/valueThe research using the diffusion of innovation paradigm, the combination of market-based and knowledge-based paradigms is expected to fill the previous research gap and become the first uniqueness and originality, as well as characteristic of this research. The second originality is this research examines the role of the knowledge management practice variable as the moderator variable. Then, the third originality is this research investigates the relationship between business partner relationship and innovation performance. These three originalities are still rarely studied in previous studies, so this research will further complement, expand the study, especially in the knowledge management, innovation performance area.
Effect of on-farm testing on adoption of banana production technologies among smallholder farmers in Meru region, Kenya
Authors: Kirimi Florence Kaumi , Onyari Charles Nyambane , Njeru Lucy Karega , Mogaka Hezron Rasugu 
Published: September 2021

PurposeThe purpose of this paper is to investigate the effect of on-farm testing on the adoption of banana production technologies among smallholder farmers in the Meru region, Kenya.Design/methodology/approachThe study adopted a pragmatic paradigm and a cross-sectional survey design, sampling 370 and 30 farmers proportionately from 269,499 to 19,303 smallholder banana farmers in Meru and Tharaka-Nithi Counties of Kenya, respectively.FindingsThe study revealed that there was an association between belonging to a banana farming testing group and the adoption of banana technology. The study also revealed that most farmers were not interested in adopting banana technologies as they preferred the use of conventional methods, due to unstable market prices, lack of subsidized banana production input, inaccessibility to technological materials, few extension experts and lack of enough demonstrations.Research limitations/implicationsSome respondents were not willing to freely offer the information required for this study. This was delimited by assuring the informants of the confidentiality of their responses.Originality/valueThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The agricultural extension service providers will have more light on the underlying issues that need to be considered if meaningful interventions are to be done on various aspects of the banana value chain.
From “personal” to “interpersonal”: a multilevel approach to uncovering the relationship between job satisfaction and knowledge sharing among IT professionals
Authors: Lei Yang , Zhou Qiang , Ren Jifan , Cui Xiling 
Published: September 2021

PurposeThis study aims to examine how job satisfaction (JS) affects two types of knowledge sharing (KS), in-role KS and extra-role KS. It also investigates the mediating effect of knowledge sharing self-efficacy (KSSE) and the moderating effect of team collaborative culture (TCC) between JS and two types of KS.Design/methodology/approachThis study applies attribution theory to develop a cross-level model and validate it through paired data collected from 322 information technology professionals nested within 80 teams. Hierarchical linear modeling is used to test the hypotheses.FindingsJS positively influences in-role and extra-role KS via KSSE and TCC positively moderates the relationship between JS and extra-role KS.Originality/valueThis study is one of the first to investigate the mechanism underlying the influence of JS on two types of KS. It also identifies the mediating and moderating effects of this mechanism.
Institutional complexity and family business development: a case study of the Charoen Pokphand group
Authors: Wang Bo , Liang Qiang , Song Lihong , Xu Erming 
Published: September 2021

Purpose
With features of both “family” and “business,” family businesses must seek a balance between the emotional aspect of “family” and the economic aspect of “business” in its organizational and decision-making processes to ensure the sustainability of the family’s entrepreneurship. This study aims to focus on how internal institutional complexity combined evolves alongside the growth of the family business.

Design/methodology/approach
The research looks, from the perspective of institutional logic, into the Charoen Pokphand Group, which is an epitome of overseas Chinese family businesses and proceeds to build a model of family business growth in the context of institutional complexity.

Findings
The research finds that as a family business grows, institutional complexity inside the organization would change from aligned period to sustaining period and then to dominant period. Then further elucidates the process of proactive response in different stages of the development of a family business. Attaching equal importance to the cultivation of entrepreneurship and to the continuation of family values and culture is the crucial mechanism by which Chinese family businesses seek a balance between family logic and business logic.

Originality/value
This paper unveils the change of institutional complexity in the evolution of family businesses and the process of action of its agency as an organization, and simultaneously partly reveals the features of entrepreneurship that overseas Chinese family businesses have as they grew, which is of positive significance for exploring and building a path of growth unique to Chinese family businesses.
Descriptive Data Analysis of Inequality of Economic Opportunity using the Queensland Family Cohort Pilot Study
Authors: Gannon Brenda , Jayawardana Danusha , Clifton Vicki 
Published: September 2021

AbstractThe Queensland Family Cohort Pilot Study follows 450 women over the course of their pregnancy and after giving birth, collecting a wealth of information on socioeconomic characteristics, health, healthcare use and biological samples. The focus of this paper is to demonstrate how these data may be used to measure inequality of opportunity, between advantaged and disadvantaged populations, for mothers, partners and babies, in terms of their mental health and use of scarce health care resources. This paper provides the foundation for future analyses when a wider Queensland study is proposed to collect data from 12,500 families.
Indigenising the curriculum: Transcending Australian geography’s dark past
Authors: Williams Stewart , Anders Robert , Vreugdenhil Roger , Byrne Jason 
Published: September 2021

AbstractAustralian geography has been implicated in the White settler colonial project, including in the dispossession of Aboriginal peoples from their lands and the denigration of Aboriginal knowledges. Recognising the harm caused by this racist past is crucial if geography education is to play a stronger role in decolonisation. Much work is already underway. This article reports on a process to Indigenise the curriculum, building a partnership between Aboriginal people and Australian geography educators at the University of Tasmania. Tasmanian geographers and spatial scientists have begun working with Aboriginal people to come to terms with the discipline’s history of genocide, dispossession and cultural politics of extinction. A workshop that was the foundation for this process generated valuable insights into how to include the knowledge and practices of Aboriginal peoples in geography and planning curricula; build communities of practice; and learn to live lawfully and lore‐fully on Country. Lessons learned have informed curriculum renewal and are helping to build new relationships.
Mineral content, fatty acid composition, and volatile compounds of gluten‐free tarhana formulated with different cereal and pulse flours
Authors: Çalışkan Koç Gülşah , Tekgül Yeliz , Erten Edibe Seda , Akdoğan Arda 
Published: September 2021

AbstractThis research was intended to determine the effect of different cereal and pulse flours and commercial gluten‐free flour on the mineral content, fatty acid composition, and volatile compounds of the gluten‐free tarhana (cereal‐based fermented dry soup). In order to produce the gluten‐free tarhana, white bean (BF), chickpea (CF), commercial gluten‐free (GWF), yellow lentil (LF), and rice (RF) flours were used. The Mg, K, Al, and Mn contents of tarhanas obtained using different pulse and cereal flours were found to be quite high compared to tarhanas produced with commercial GWF. The utilization of different cereal and pulse flours in the tarhana formulation resulted in an increase in the percentage (30.37–51.47%) of the total polyunsaturated fatty acid (TPUFA). The highest (452.4 µg/g) and the lowest (241.17 µg/g) total concentration of all compounds were detected in BF and GWF, respectively. The highest number (21) of compounds belonged to terpenes and terpenoids, followed by acids, hydrocarbons, alcohols, aldehydes, esters, ketones, and alkanes.Practical ApplicationThis study shows that cereal and pulse flours can be used to produce acceptable tarhana with improved nutritional quality in terms of mineral and fatty acid contents. The results of the current study may be useful and applicable to food manufacturers producing gluten‐free products for celiac patients.