bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reimagining “Defeat” in the Transnational West: John Newman Edwards, Mexican Exile, and the Confederate Experiment 2.0
Authors: Hulbert Matthew Christopher 
Published: March 2021

Abstract
Rather than surrendering to Union forces in 1865, various bands of ex-Confederates chose Mexican exile. From generals and elite politicians to rank-and-file soldiers, the majority of these “Confederados” journeyed to French-controlled Mexico to escape punishment, to tap financial opportunities, and to observe how southern society would function post-emancipation. Still others, as represented by the cavalry officer and Quixotic newspaper editor John Newman Edwards, understood the U.S. Civil War on more international terms. To these men, Mexico constituted a new, imperially subsidized laboratory to continue the Confederate Experiment and recreate a mythic version of the Old South. Although cut short by the violent death of Emperor Maximilian I, their saga reveals not only how adaptation to Confederate defeat took different forms in the immediate postbellum period, but also the extent to which conceptions of defeat and even the purpose of the Confederacy itself had never been monolithic in the first place.
Eosinophilic asthma and the role of monoclonal antibodies
Authors: Jarrell Lynda 
Published: March 2021

Abstract:
Eosinophilic asthma presents with continuous airway inflammation resistant to inhaled corticosteroids but responsive to oral glucocorticoids and monoclonal antibodies. Diagnostic criteria include significantly elevated blood or sputum eosinophils and/or fractional exhaled nitric oxide. Five monoclonal antibodies are used for treatment, with accurate diagnosis and early intervention essential to better outcomes.
Analysis of the concise etiology of pleural effusion in 3707 pediatric patients in a single clinical center
Authors: Wang Gang , Wang Yi , Wu Chun , Pan Zhengxia , Wang Junke , Wu Yuhao , Wang Quan , Li Yonggang , Dai Jiangtao 
Published: March 2021

Purpose:
To summarize the concise etiology of pleural effusion in 3707 patients over a period of 13 years at the Children’s Hospital of Chongqing Medical University.

Methods:
A total of 3707 patients with pleural effusion were included from January 2007 to December 2019 at our hospital. According to etiology, pleural effusion was divided into infectious pleural effusion and noninfectious pleural effusion. The infection factors were further subdivided as being caused by tuberculous, paragonimiasis, parapneumonic factors (including multiple bacterias, multiple viruses, mycoplasma, chlamydia, chlamydia, fungus and fever, blood routine examination shows increased white blood cells and neutrophils). The noninfectious factors were further subdivided into neoplastic, trauma, other causes. They were also divided into the infants and young children group (3 y and below), the preschool group (3–6 y) and the school-age group (above 6 y) depending on age.

Results:
Among the 3707 children, there were 2925 (78.9%) patients with infectious pleural effusions, and 782 (21.1%) patients with noninfectious pleural effusions. The incidence of infectious pleural effusions caused by parapneumonic factors decreased significantly with increasing age; however, parapneumonic factors remained the primary etiology of infectious pleural effusion in each group. The incidence of infectious pleural effusions caused by tuberculosis and paragonimiasis increased significantly with increasing of age. Tumor was the primary cause of noninfectious pleural effusion. The incidence of noninfectious pleural effusion caused by tumor and other causes increased significantly with increasing age. The incidence of noninfectious pleural effusion caused by trauma in the preschool group was significantly greater than that of the infants and young children group and school-age group. The number of noninfectious pleural effusion caused by other causes in the school-age group were significantly greater than those of in the infants and young children group and preschool group.

Conclusion:
In our center, pediatric pleural effusion was primarily caused by infections. parapneumonic factors were the primary causes of infectious pleural effusions. The proportion of noninfective pleural effusions in the etiology of pleural effusion in children was small; however, some were difficult to treat, requiring long-term treatment and possibly poor prognosis. Therefore, multidisciplinary treatment should be combined.
Association of equol with obesity in postmenopausal women
Authors: Takahashi Atsushi , Anzai Yukio , Tanji Nobuo , Imaizumi Hiromichi , Fujita Masashi , Hayashi Manabu , Abe Kazumichi , Ohira Hiromasa 
Published: March 2021

Abstract

Objective:
Equol, which is an active metabolite of isoflavone, has a beneficial impact on metabolic diseases such as dyslipidemia and hyperglycemia. However, the effect of equol on obesity remains uncertain. This study was performed to determine the association between equol and obesity in postmenopausal women.

Methods:
We evaluated 386 women in their 50s-60s who underwent health check-ups from February 2018 to January 2019 at Watari Hospital Health Center in Fukushima, Japan. Overweight and visceral obesity were defined as a body mass index ≥25 kg/m2 and waist circumference ≥ 90 cm, respectively. Participants were asked to complete a questionnaire about their ordinary lifestyle. Participants were defined as equol producers when urinary equol level was 1.0 μM or more. The proportion of individuals with obesity (overweight and visceral obesity) and lifestyle factors were compared between equol producers and nonproducers. In addition, the association between equol and obesity was examined using logistic regression analysis with adjustment for lifestyle factors.

Results:
Of the 386 participants, 106 (27.5%) women were equol producers. The proportions of women who were overweight (13.2% vs 25.7%) and had visceral obesity (6.6% vs 20.7%) were significantly lower in the equol-producing group than in the nonproducing group. Multivariable logistic regression analysis showed that equol production was significantly associated with overweight (odds ratio =0.47, 95% confidence interval: 0.25-0.88) and visceral obesity (odds ratio =0.30, 95% confidence interval: 0.13-0.68).

Conclusions:
Equol is significantly associated with obesity in postmenopausal women.

Video Summary:
http://links.lww.com/MENO/A727.
Momentary pain assessments reveal benefits of endoscopic discectomy: a prospective cohort study
Authors: Wakaizumi Kenta , Wu Binbin , Huang Shishi , Fan Linyu , Shen Bangli , Wu Bo , Zhang Jing , Baliki Marwan N. , Apkarian A. Vania , Huang Lejian 
Published: March 2021

Introduction:
Lumbar disc herniation (LDH) is a common back disorder that evokes back and/or leg pain. Percutaneous endoscopic lumbar discectomy (PELD) is a minimally invasive surgery for patients with LDH. However, there is little evidence of effectiveness of PELD compared with conservative treatments.

Objective:
The goal of this study was to quantify the efficacy of PELD compared with conservative treatments.

Methods:
Here, we conducted a prospective observational cohort study using momentary pain assessments via a smartphone app during 3 months following surgery. The trajectories of daily ecological momentary pain assessments were fitted with an exponential model containing two parameters: a pain reduction coefficient and the percentage of persistent pain. To control for selection bias between PELD and Conservative groups (N = 167 and 34), we used inverse probability (IP) of treatment weighting for statistical comparisons.

Results:
Compared with conservative treatments, both momentary pain rating and the exponential model showed statistically significant pain recovery following PELD (p < 0.001). In addition, PELD had a faster pain recovery rate (hazard ratio (95% confidence interval): 1.75 (1.40, 2.20), p < 0.001), greater overall pain recovery rate (odds ratio (95%CI): 2.35 (2.01, 5.26), p < 0.001), faster pain reduction (t199 = 3.32, p = 0.001), and lower estimated persistent pain (Z = 2.53, p = 0.011). Greater pain intensity and lower anxiety before the surgery were predictors of faster pain reduction in the recovery subgroup following PELD.

Conclusions:
In conclusion, momentary pain rating and the model fitting revealed that PELD provided rapid pain recovery that lasted for at least three months. Greater pain intensity and lower anxiety before the surgery were predictors of faster pain reduction in the recovery subgroup following PELD. Daily momentary pain rating on a smartphone may be able to provide more informative data to evaluate effect of an intervention than pain assessment on hospital visits.
Genomic and Chemical Diversity of Commercially Available Industrial Hemp Accessions
Authors: Johnson Matthew S. , Wallace Jason G. 
Published: March 2021

AbstractHigh consumer demand for cannabidiol (CBD) has made industrial hemp (Cannabis sativa) an extremely high-value crop. However, high demand has resulted in the industry developing faster than the research, resulting in the sale of many hemp accessions with inconsistent performance and chemical profiles. To determine the genetic and phenotypic consistency in available CBD hemp varieties, we obtained seed or clones from 22 different named accessions. Genotypes (~48,000 SNPs) and chemical profiles (% CBD and THC by dry weight) were determined for up to 8 plants per accession. Many accessions--including several with the same name--showed little consistency either genetically or chemically. Most seed-grown accessions deviated significantly from their purported levels of CBD and THC based on the supplied certificates of analysis. Several also showed evidence of an active tetrahydrocannabinolic acid (THCa) synthase gene, leading to unacceptably high levels of THC in female flowers. We conclude that the current market for CBD-hemp varieties is highly unreliable, making many purchases risky for growers. We suggest options for addressing these issues, such using unique names and developing seed and plant certification programs to ensure the availability of high-quality, verified planting materials.
Peer presence increases adolescents’ prosocial behavior by speeding the evaluation of rewards for others
Authors: Sullivan Nicolette J. , Li Rosa , Huettel Scott A. 
Published: March 2021

AbstractPeer presence can elicit maladaptive adolescent decision-making, potentially by increasing sensitivity to the rewards one receives. It remains unknown whether peer presence also increases adolescents’ sensitivity to others’ rewards, which could have an adaptive effect in contexts allowing pro-social behaviors. Here, we combine social utility modeling and real-time decision process modeling to characterize how peer presence alters adolescents’ processing of self and other rewards. We found that adolescents behaved selfishly when privately allocating rewards for themselves and a peer in an incentive-compatible task. In peer presence, however, adolescents became more altruistic. Real-time estimates of reward processing collected using computer mouse tracking showed that altruistic behavior was associated with relatively earlier processing of peer-rewards relative to self-rewards, such that peer presence sped peer-reward processing without altering self-reward processing. Our results indicate a causal mechanism through which peer presence prompts greater prosocial behavior by altering how adolescents process prosocial outcomes.
Dopamine and reward-related vigor in younger and older human participants
Authors: Hird E. J. , Beierholm U. , De Boer L. , Axelsson J. , Riklund K. , Nyberg L. , Beckman L. , Guitart-Masip M. 
Published: March 2021

AbstractVigor reflects how motivated one is to respond to a stimulus. We previously showed that humans are more vigorous when more reward is available on average, and that this relationship is modulated by the dopamine precursor levodopa. Dopamine signalling and probabilistic reward learning degrade with age, so the relationship between vigor and reward should change with age. We test this and assess whether the relationship between vigor and reward correlates with D1 dopamine receptor availability measured using Positron Emission Tomography. We measured response times of 30 older and 30 younger subjects during an oddball discrimination task where rewards varied systematically between trial. Reward rate had a similar impact on the vigor of both groups. We observed a weak positive association across subjects between ventral striatal dopamine receptor availability and effect of average reward rate on response time, which was in the opposite direction to our prediction. Overall, the effect of reward on response vigor is similar between younger and older humans and is weakly sensitive to dopamine D1 receptor availability.
Pro-autoimmune allele of tyrosine phosphatase, PTPN22, enhances tumor immunity
Authors: Orozco Robin C. , Marquardt Kristi , Mowen Kerri , Sherman Linda A. 
Published: March 2021

AbstractThe 1858C>T allele of the tyrosine phosphatasePTPN22 (causing amino acid substitution R620W in encoded protein Lyp) is present in 5-10% of the North American population and is strongly associated with numerous autoimmune diseases. Although much research has been done to define how this allele potentiates autoimmunity, the influencePTPN22and its pro-autoimmune allele has in tumor immunity is poorly defined. To interrogate the role this allele may have in the anti-tumor immune response, we used CRISPR/Cas9 to generate mice in which the ortholog of Lyp, PEP, is mutated at position 619 to produce the relevant pro-autoimmune mutation (R619W). Results of this study show that mice homozygous for this alteration (PEP-619WW) resist tumor growth as compared with wildtype mice. Consistent with these results, tumors from PEP-619WW mice have more CD45 infiltrates containing more activated CD8 T cell and CD4 T cells. Additionally, there are more cDC1 cells and less MDSCs in tumors from PEP-619WW mice. Interestingly, the tumor infiltrating PEP-619WW cDC1s have decreased PD-L1 expression compared to cDC1s from PEP-WT mice. Taken together, our data show that the pro-autoimmune allele ofPtpn22drives a strong anti-tumor response in innate and adaptive immune cells resulting in superior control of tumors.
Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia
Authors: Miura Yoko , Lam Maggie , Bourke Jane E. , Kanazawa Satoshi 
Published: March 2021

ABSTRACTIdiopathic pulmonary fibrosis (IPF) is pathologically classified by usual interstitial pneumonia (UIP). Conventional bleomycin models used to study pathogenic mechanisms of pulmonary fibrosis display transient inflammation and fibrosis so their relevance to UIP is limited. We developed a novel chronicinduced-UIP (iUIP) model, inducing fibrosis in D1CC×D1BC transgenic mice by intra-tracheal instillation ofbleomycin mixed withmicrobubbles followed bysonoporation (BMS). A bimodal fibrotic lung disease was observed over 14 weeks, with an acute phase similar to nonspecific interstitial pneumonia (NSIP), followed by partial remission and a chronic fibrotic phase with honeycombing similar to UIP. In this secondary phase, we observed poor vascularization despite elevated PDGFRβ expression. γ2PF- and MMP7-positive epithelial cells, consistent with an invasive phenotype, were predominantly adjacent to fibrotic areas. Most invasive cells were Scgb1a1 and/or keratin 5 positive. This iUIP mouse model displays key features of IPF and has identified potential mechanisms contributing to the onset of NSIP and progression to UIP. The model will provide a useful tool for the assessment of therapeutic interventions to oppose acute and chronic fibrosis.Summary blurbA novel mouse model of induced-usual interstitial pneumonia shows bimodal fibrosis with honeycombing, similar to chronic lung disease seen with idiopathic pulmonary fibrosis.
Assigning confidence to structural annotations from mass spectra with COSMIC
Authors: Hoffmann Martin A. , Nothias Louis-Félix , Ludwig Marcus , Fleischauer Markus , Gentry Emily C. , Witting Michael , Dorrestein Pieter C. , Dührkop Kai , Böcker Sebastian 
Published: March 2021

AbstractUntargeted metabolomics experiments rely on spectral libraries for structure annotation, but these libraries are vastly incomplete;in silicomethods search in structure databases but cannot distinguish between correct and incorrect annotations. As biological interpretation relies on accurate structure annotations, the ability to assign confidence to such annotations is a key outstanding problem. We introduce the COSMIC workflow that combines structure database generation,in silicoannotation, and a confidence score consisting of kernel density p-value estimation and a Support Vector Machine with enforced directionality of features. In evaluation, COSMIC annotates a substantial number of hits at small false discovery rates, and outperforms spectral library search for this purpose. To demonstrate that COSMIC can annotate structures never reported before, we annotated twelve novel bile acid conjugates; nine structures were confirmed by manual evaluation and two structures using synthetic standards. Second, we annotated and manually evaluated 315 molecular structures in human samples currently absent from the Human Metabolome Database. Third, we applied COSMIC to 17,400 experimental runs and annotated 1,715 structures with high confidence that were absent from spectral libraries.
Distinct Hypoxia-induced Translational Profiles of Embryonic and Adult-derived Macrophages
Authors: Wilcox Nicholas S. , Yarovinsky Timur O. , Pandya Prakruti , Ramgolam Vinod S. , Moro Albertomaria , Wu Yinyu , Nicoli Stefania , Hirschi Karen K. , Bender Jeffrey R. 
Published: March 2021

SummaryTissue homeostasis and repair are orchestrated by resident and newly recruited macrophages that alter their gene expression program in response to changes in tissue microenvironment. Embryonic macrophages, such as fetal liver derived macrophages (FLDM) seed the organs, including heart and lung during embryonic development and persist throughout the adult lifetime, while bone marrow-derived macrophages (BMDM) are recruited following an acute perturbation. Transcriptome analyses of FLDM and BMDM identified differences between them at the level of RNA expression, which correlates imperfectly with protein levels. Post-transcriptional regulation by microRNAs (miRNAs) and RNA-binding proteins determines mRNA stability and translation rate and may override transcriptional cues in response to environmental changes, such as hypoxia. To identify distinct features of FLDM and BMDM response to hypoxia at the level of translation, we employed translating ribosome affinity purification (TRAP) to isolate polysomal RNA. RNA-seq profiling of translated RNA identified distinct hypoxia-induced translational signature of BMDM (Ly6e, vimentin and glycolysis-associated enzymes Pgk1, Tpi1, Aldoa, Ldha) and FLDM (chemokines Ccl7 and Ccl2). By translational profiling of BMDM and FLDM with deletion of the RNA-binding protein HuR, we identified transcripts that were dependent on HuR. These findings highlight the importance of HuR and identify its distinct targets for post-transcriptional regulation of gene expression in embryonic vs. adult-derived macrophages.
Differential responses of amphibians and reptiles to land-use change in the biodiversity hotspot of north-eastern Madagascar
Authors: Fulgence Thio Rosin , Martin Dominic Andreas , Randriamanantena Romual , Botra Ronick , Befidimanana Erosiniot , Osen Kristina , Wurz Annemarie , Kreft Holger , Andrianarimisa Aristide , Ratsoavina Fanomezana Mihaja 
Published: March 2021

AbstractLarge expanses of tropical rainforest have been converted into agricultural landscapes cultivated by smallholder farmers. This is also the case in north-eastern Madagascar; a region that retains significant proportions of forest cover despite slash-and-burn hill rice cultivation and vanilla agroforestry expansion. The region is also a global hotspot for herpetofauna diversity, but how amphibians and reptiles are affected by land-use change remains largely unknown. Using a space-for-time study design, we compared species diversity and community composition across seven prevalent land uses: unburned (old-growth forest, forest fragment, and forest-derived vanilla agroforest) and burned (fallow-derived vanilla agroforest, woody fallow, and herbaceous fallow) land-use types, and rice paddy. We conducted six comprehensive, time-standardized searches across at least ten replicates of each land-use type and applied genetic barcoding to confirm species identification. We documented an exceptional diversity of herpetofauna (119 species; 91% endemic). Plot-level amphibian species richness was significantly higher in old-growth forest than in all other land-use types. Plot-level reptile species richness was significantly higher in unburned land-use types compared to burned land-use types. For both amphibians and reptiles, the less-disturbed land-use types showed more uneven communities and the species composition in old-growth forest differed significantly from all other land-use types. Amphibians had higher forest dependency (38% of species occurred exclusively in old-growth forest) than reptiles (26%). Our analyses thus revealed that the two groups respond differently to land-use change: we found less pronounced losses of reptile species richness especially in unburned agricultural habitats, suggesting that reptiles are less susceptible to land-use change than amphibians, possibly due to their ability to cope with hotter and drier microclimates. Overall, old-growth forest harboured a unique diversity, but some species also thrived in vanilla agroforestry systems, especially if these were forest-derived. This highlights the importance of conserving old-growth forests and non-burned land-use types within agricultural landscapes.
Copper dependent ERK1/2 phosphorylation is essential for the viability of neurons and not glia
Authors: Chakraborty Kaustav , Kar Sumanta , Rai Bhawana , Bhagat Reshma , Naskar Nabanita , Seth Pankaj , Gupta Arnab , Bhattacharjee Ashima 
Published: March 2021

AbstractIntracellular Copper [Cu(I)] has been hypothesized to play role in the differentiation of the neurons. This necessitates understanding the role of Cu(I) not only in the neurons but also in the glia considering their anatomical proximity, contribution towards ion homeostasis, neuronal physiology, and neurodegeneration. In this study we did a systematic investigation of the changes in the cellular copper homeostasis during neuronal and glial differentiation and the pathways triggered by them. Our study demonstrates increased mRNA for the plasma membrane copper transporter CTR1 leading to an increased Cu(I) during neuronal (PC-12) differentiation. ATP7A is retained in the Trans Golgi Network (TGN) despite high Cu(I) demonstrating its utilization in triggering the pathways towards the neuronal differentiation. One of these pathways is ERK1/2 phosphorylation accompanying the differentiation of both PC-12 and human fetal brain derived neuronal progenitor cells. The study demonstrates that the ERK1/2 phosphorylation is essential for the viability of the neurons. In contrast, differentiated C-6 (glia) cells contain low intracellular copper and significant downregulation of the ERK1/2 phosphorylation. Interestingly ATP7A shows vesicular localization despite the low copper in the glia. In addition to the TGN in the perinuclear region, ATP7A localizes into RAB11 positive recycling endosomes in the glial neurites, not observed in the neurons. Our study demonstrates role of the copper dependent ERK1/2 phosphorylation in the neuronal differentiation. Whereas glial differentiation largely involves sequestration of Cu(I) into the endosomes potentially (i) for ready release to the neurons (ii) rendering cytosolic copper unavailable for pathways like the ERK1/2 activation.