bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meta-Analysis of RNA-Seq Studies Reveals Genes With Dominant Functions During Flower Bud Endo- To Eco-Dormancy Transition in Prunus Species
Authors: Canton Monica , Forestan Cristian , Bonghi Claudio , Varotto Serena 
Published: January 2021

Abstract
In deciduous fruit trees, entrance into dormancy occurs in later summer/fall, concomitantly with the shortening of day length and decrease in temperature. Dormancy can be divided into endodormancy, ecodormancy and paradormancy. In Prunus species flower buds, entrance into the dormant stage occurs when the apical meristem is partially differentiated; during dormancy, flower verticils continue their growth and differentiation. Each species and/or cultivar requires exposure to low winter temperature followed by warm temperatures, quantified as chilling and heat requirements, to remove the physiological blocks that inhibit budburst. A comprehensive meta-analysis of transcriptomic studies on flower buds of sweet cherry, apricot and peach was conducted, by investigating the gene expression profiles during bud endo- to ecodormancy transition in genotypes differing in chilling requirements. Conserved and distinctive expression patterns were observed, allowing the identification of gene specifically associated with endodormancy or ecodormancy. In addition to the MADS-box transcription factor family, hormone-related genes, chromatin modifiers, macro- and micro-gametogenesis related genes and environmental integrators, were identified as novel biomarker candidates for flower bud development during winter in stone fruits. In parallel, flower bud differentiation processes were associated to dormancy progression and termination and to environmental factors triggering dormancy phase-specific gene expression.
Discovery of A New Mammal Species (Soricidae: Eulipotyphla) From Narcondam Volcanic Island, India
Authors: Kamalakannan Manokaran , Sivaperuman Chandrakasan , Kundu Shantanu , Gokulakrishnan Govindarusu , Venkatraman Chinnadurai , Chandra Kailash 
Published: January 2021

Abstract
The present study discovered the existence of a new Crocidura species of shrew (Soricidae: Eulipotyphla) from Narcondam Island, India by using both morphological and molecular approaches. The new species, Crocidura narcondamica sp. nov. is medium-sized and has a distinct external morphology (darker-grey dense fur with a thick and darker tail) and craniodental (braincase is rounded and elevated with weak lambdoidal ridges) characters in comparison with other close congeners. This description illuminates the first discovery of soricid fauna (shrew) from this volcanic island and a total of 12 Crocidura species catalogued in the Indian checklist of mammals. The newly discovered species maintained sufficient genetic distances (12% to 16.6%) with other Crocidura species known from the Indian mainland, Andaman and Nicobar Archipelago, and Myanmar. Both Maximum-Likelihood and Bayesian phylogeny showed distinct clustering of all soricid species and exhibited congruence with the previous evolutionary hypothesis. The present phylogenetic analyses also furnished the oldest evolutionary lineages of this newly discovered species in comparison with other congeners, which assumed to be possible colonization of this species due to immature radiation in Narcondam Island.
Effect of Revascularization on Coronary Chronic Total Occlusion in Patients Undergoing Coronary Artery Bypass Grafting
Authors: Kim Yun Seok , Lee Jiyun , Kim Hwan Wook , Kang Joonkyu , Song Hyun , Lim Ju Yong 
Published: January 2021

Abstract
Background: Bypass grafting for chronic total occlusions remains surgically challenging and controversial. Therefore, we evaluated the incidence and clinical outcomes of revascularization on chronic total occlusions undergoing coronary artery bypass surgery.Methods: Among 828 patients who underwent isolated coronary artery bypass surgery from January 2010 to December 2018, 245 patients (29.5%) diagnosed with at least one chronic total occlusion were included and retrospectively reviewed. Primary endpoints were 30-day and overall mortality. Secondary endpoint was the composite outcome of major adverse cardiac and cerebrovascular events. Results: With a mean follow-up of 56.6 ± 6.5 months in 245 patients with chronic total occlusions, 51 patients (20.9%) received incomplete revascularization for chronic total occlusions. Risk factor analysis showed that incomplete revascularization was associated with increased 30-day (odds ratio 8.62; 95% confidence interval (CI) 1.64 – 50; p = 0.011) and overall mortality (hazard ratio (HR) 2.13; 95% CI 1.07 – 4.21; p = 0.03). ICR also increased the risk of major adverse cardiac and cerebrovascular events (HR 1.98; 95% CI 1.12 – 3.54; p = 0.01). Freedom from overall mortality was 92.8%, 90.4%, and 86.8% in the complete revascularization group, and 86.3%, 80.0%, and 72.7% in the incomplete revascularization group, at 1, 3, and 5 years, respectively (p = 0.004).Conclusions: In patients with chronic total occlusions undergoing coronary artery bypass surgery, the rate of incomplete revascularization was 20.9%, and it significantly increased the risk of mortality and major adverse cardiac and cerebrovascular events. Further studies in a large cohort are needed.
Postoperative Stroke in Patients After Spinal Anesthesia and Disparate Response Patterns of Carotid or Cerebral Blood Flow and Baroreflex Functionality to Spinal Bupivacaine in Rats
Authors: Poon Yan-Yuen , Liu Yueh-Wei , Huang Ya‐Hui , Chan Samuel H.H. , Tsai Ching-Yi 
Published: January 2021

Abstract
Spinal anesthesia is generally accepted as an effective and safe practice. Three rare incidents of postoperative cerebral infarction after surgery under spinal anesthesia with bupivacaine prompted us to assess whether spinal bupivacaine may impact carotid or cerebral blood flow and baroreflex functionality. We found that all three patients shared common pathology of stenosis or atheromatous lesions in the carotid or middle cerebral artery. In a companion animal study, we further observed that subarachnoid application of bupivacaine that reached low thoracic spinal cord in male Sprague‐Dawley rats elicited an initial (Phase I) reduction in mean arterial pressure (MAP), carotid blood flow (CBF) and baroreflexmediated sympathetic vasomotor tone, all of which returned to baseline in Phase II. Whereas heart rate (HR) exhibited sustained reduction, cardiac vagal baroreflex, baroreflex efficiency index (BEI) and tissue perfusion and oxygen in cerebral cortex supplied by middle cerebral artery remained unaltered during both phases. However, in one‐third of animals studied, Phase II gave way to a Phase III characterized by secondary hypotension and depressed baroreflex‐mediated sympathetic vasomotor tone, along with continuous decline in HR, sustained cardiac vagal baroreflex, decreased BEI, and reduction in CBF and tissue perfusion or oxygen in cerebral cortex. We conclude that carotid and cerebral blood flow can be compromised after spinal anesthesia, and impaired baroreflex‐mediated sympathetic vasomotor tone that leads to hypotension plays a contributory role.
The Causes of Chest Pain and the Positive Rate of Myocardial Enzyme Testing in Diagnosis of Chest Pain in Children
Authors: Chen Li , Duan Hongzhou , Li Xiaoyan , Yang Zuozhen , Jiao Meng , Sun Kangtai , Jin Mei 
Published: January 2021

Abstract
Background Chest pain is a common complaint at pediatric cardiology clinics and often leads to an extensive cardiac evaluation. In this study, we analyzed the causes of chest pain in Chinese children and developed criteria for targeted myocardial enzyme testing.Methods We retrospectively analyzed the clinical data of patients aged below 18 years visiting our hospital for chest pain between 2005 and 2019. Based on auxiliary exams and clinical diagnosis, we analyzed the positive rate of myocardial enzyme testing in identifying the causes of chest pain in children.Results A total of 7251 children were included in this study. The chest pain was of cardiac origin in 581 patients (8.0%). The incidence of noncardiac chest pain was significantly higher in the preschool group and the school-age group than in the adolescent group (93.5% vs 93.8% vs 90.3%, P < 0.05). Among children with cardiac chest pain, the most common concomitant symptom was chest tightness (67.0%). Myocardial enzyme testing was performed in 5408 patients and was abnormal in 453 (8.4%) patients.Conclusions In children, chest pain is mostly benign and rarely cardiac. During diagnosis, targeted myocardial enzyme testing based on medical history and physical examination can effectively reduce resource use.
Impulsive Selfish and Contemplated Prosocial Behavior in Children
Authors: Ishihara Toru , Morita Noriteru , Takagishi Haruto , Kamijo Keita 
Published: January 2021

Abstract
Cooperation is required for human beings to survive and thrive. In the past decade, to deepen the understanding of human cooperation, more attention has been paid to default prosocial behavior and calculated selfish behavior in the adult population. Whether prosocial behavior is due to an intrinsically altruistic nature or to internalized social norms remains controversial. We approached this question by examining the relationship between cognitive control and decision time, and prosocial behavior in children. We analyzed the data obtained from 226 children aged from 8 to 11 years. The results indicated that greater cognitive control and longer decision time were independently associated with promoted prosocial behavior. That is, the intuitive cooperation model of prosocial behavior established in the adult population may not be supported in children. Although cognitive control was positively associated with prosocial behavior, even children with poor cognitive control promoted prosocial behavior when they were given sufficient decision time. Our findings support the view that prosocial behaviors are automated as habits by the internalization of social norms through lived experiences, and that human beings have an intrinsically selfish nature.
Buffalo Sperm Surface Proteome Profiling Reveals an Intricate Relationship Between Innate Immunity and Reproduction
Authors: Batra Vipul , Bhushan Vanya , Ali Syed , Sarwalia Parul , Solanki Subhash , Kumaresan Arumugam , Kumar Rakesh , Datta Tirtha 
Published: January 2021

Abstract
BackgroundLow conception rate (CR) despite insemination with morphologically normal spermatozoa is a common reproductive restraint which limits buffalo productivity. This accounts for a significant loss to the farmers and the dairy industry, especially in agriculture-based economies. The immune-related proteins on the sperm surface are known to regulate fertility by assisting the spermatozoa in their survival and performance in the female reproductive tract (FRT). Regardless of their importance, very few studies have specifically catalogued the buffalo sperm surface proteome. The study was designed to determine the identity of sperm surface proteins and to ascertain if the epididymal expressed beta-defensins (BDs), implicated in male fertility, are translated and applied onto buffalo sperm surface along with other immune-related proteins. ResultsThe raw mass spectra data searched against in-house generated proteome database from UniProt using Comet search engine identified more than 300 proteins on the ejaculated buffalo sperm surface which were bound either by non-covalent (ionic) interactions or by a GPI-anchor. The singular enrichment analysis (SEA) revealed that most of these proteins were extracellular with varied binding activities and were involved in either immune or reproductive processes. Flow cytometry using six FITC-labelled lectins confirmed the prediction of glycosylation of these proteins. Several beta-defensins (BDs), the anti-microbial peptides including the BuBD-129 and 126 were also identified amongst the buffalo sperm surface proteins. The presence of these proteins was confirmed by immunocytochemistry and IVF experiments. Conclusions The surface of the buffalo spermatozoa is heavily glycosylated because of the epididymal secreted glycoproteins like BDs and the GPI-anchored proteins. The glycosylation pattern, however, could be perturbed in the presence of elevated salt concentration or incubation with PI-PLC. The identification of numerous BuBDs on the sperm surface strengthens our hypothesis that these BDs assist the buffalo spermatozoa either in their survival or in performance in the FRT. Our results suggest that BuBD-129 is a sperm-surface BD which could have a role in buffalo sperm function. Further studies elucidating its exact physiological function are required to better understand its roles in the regulation of male fertility.
Endovascular Treatment of Falcine Sinus Dural Arteriovenous Fistula
Authors: Piao Jianmin , Zhao Yuhao , Yang Zhongxi , Yu Jinlu 
Published: January 2021

Abstract
BackgroundDural arteriovenous fistula (DAVF) often occurs in the cavernous sinus, transverse sinus, sigmoid sinus, tentorium, etc. A DAVF in the falcine sinus is extremely rare in adults. DAVF treatment has progressed from microsurgery to endovascular treatment (EVT). At present, the treatment for DAVF in the falcine sinus is mainly EVT. Cases PresentationWe present 2 cases of DAVF in the falcine sinus. In case 1, the patient was a 38-year-old male, and in case 2, the patient was a 35-year-old female; both had sudden onset of cerebral hemorrhage. They underwent CT angiography (CTA) and/or digital subtraction angiography (DSA), which showed a DAVF in the falcine sinus. In case 1, the feeding artery was only the right middle meningeal artery (MMA), which drained to the superior sagittal sinus through the cortical vein. In contrast, in case 2, there were a variety of feeding arteries, including bilateral ophthalmic arteries, the internal maxillary artery and the right MMA, and the DAVF drained through both cortical veins and cerebral deep veins simultaneously. Both were cases of Cognard type IV DAVF. Using Onyx (Medtronic, Irvine, California, USA), both patients underwent complete EVT through the MMA, with a satisfactory therapeutic outcome. ConclusionWe believe that it is highly feasible to use Onyx for DAVFs in the falcine sinus using an arterial approach through the MMA to perform EVT. Nevertheless, due to the limited number of cases, we still need to accumulate additional experience.
Significance of mucin type glucosaminyl (N-acetyl) transferase 3 expression in lung squamous cell carcinoma
Authors: Chen Yu-Jun , Gao Li , Zhang Rui , Chen Gang , Feng Zhen-bo , Zhao Yong-Xiang 
Published: January 2021

Abstract
Background: The clinical significance and role of glycan synthase glucosamine (N-acetyl) transferase 3 (GCNT3) has not been investigated in lung squamous cell carcinoma (LUSC).Materials & Methods: In the present study, multiple detection technologies including tissue microarrays, external microarrays and RNA-seq were adopted for evaluating the clinic-pathological significance of GCNT3 in 1632 LUSC samples and 1478 non-cancer samples. Standard mean difference and hazard ratio value were calculated from all included datasets for assessing differential expression and prognostic value of GCNT3 in LUSC. The molecular basis underlying GCNT3 in LUSC was also explored through methylation level, genetic mutation and functional enrichment analysis of GCNT3-correlated genes in LUSC. Results: GCNT3 was obviously upregulated in LUSC samples. GCNT3 overexpression exerted unfavorable impact on the progression-free survival and overall survival of LUSC patients from GSE29013. The mRNA expression of GCNT3 was negatively correlated with methylation level of GCNT3 in LUSC and the predominant type of genetic alteration for GCNT3 in LUSC was mRNA high. Genes correlated with GCNT3 in LUSC mainly assembled in pathways such as adherens junction, p53 signaling pathway, protein digestion and absorption pathway. Conclusions: In conclusion, overexpressed GCNT3 had clinical potential as therapeutic target for LUSC.
Basic research on the effects of hyperbaric oxygen combined with hydrogen-rich saline on acute lung injury induced by lipopolysaccharide
Authors: Chang Yintao , Zhang Zhenzhen , Bao Xiaochen , Wang Mingdong , Jin Yuxiang , Zhang Si'ang , Zhao Xuewei , Fang Yiqun , Xue Lei 
Published: January 2021

Abstract
Background

To investigate the effects of hydrogen-rich saline (HRS) combined with hyperbaric oxygen (HBO) on acute lung injury (ALI) and its clinical significance.
Methods

40 adult male Sprague-Dawlay rats were randomly divided into 5 groups: the sham, LPS, LPS + HBO, LPS + HRS and LPS + HBO + HRS. LPS at a rate of 3 mg/kg was injected into the trachea of the experimental animals to develop ALI model, then the animals were respectively given simple HBO or HRS treatment or combined treatment. 3 days later, we study lung pathological, the levels of inflammatory factors, and cell apoptosis in the pulmonary tissue was detected by Tunel and cell apoptosis rate was calculated accordingly.
Results

In the groups treated with HRS and HBO, pulmonary pathological data, wet-dry weight ratio and immflammatory factors in the pulmonary tissues and avelar lavage fluid were signficantly superiror to those of the control group(P < 0.05). Cell apoptosis detection revealed that treatment with either simple HRS and HBO or combined treatment of the two could all alleviate cell apoptosis, and the combined treatment with HRS and HBO was obviously superior to single treatment༈P < 0.05༉.
Conclusions

HRS and HBO could all decrease the release of immflammatory cytokines in lung tissue, reduce accumulation of oxidative products and alleviate apoptosis of pulmoanry cells, and could produce good therapeutic effects on ALI induced by LPS. HBO combined with HRS seems to have a synergistic effect on the decrease of cell apoptosis, and in the expression of immflammatory cytokines and the generation of related immflammatory products, the combined use of HBO and HRS showed a decreasing trend as compared with simple application.
Video Intervention Therapy for Primary Caregivers in a Child Psychiatry Unit: A Randomized Feasibility Trial
Authors: Leyton Fanny , Olhaberry Marcia , Morán Javier , Cerda Cecilia de la , León María José , Sieverson Catalina , Alfaro Angela , Hernández Camila , Alvardo Rubén , Steele Howard 
Published: January 2021

Abstract
Background: During child psychiatry hospitalization, working with the families or attachment figures is a challenge, most of the children who are admitted to these units come from multi-problem families, with limited research in this area. Video Feedback (VF) intervention have proved to be a powerful resource to promote parental and child wellbeing in small children and has been used with parents with a psychiatric condition. Parental Reflective Functioning (PRF) is one of the parental abilities that can be improved with VF and could be especially important in coping with conflict and negative emotions in older children. The aim of this study is to implement Video Intervention Therapy (VIT) to enhance PRF in primary caregivers of inpatient psychiatric children. As there is no published research using VF with parents of children with severe psychopathology in a hospitalized context, this becomes a much needed pilot study providing evidence for a larger randomized control trial (RCT). Methods: The study is a single center, two-arm feasibility randomized control trial with a qualitative component. Block randomization was done to generate a 2:1 allocation, leaving more participants in the intervention group. The intervention comprises four modules; every module has both one video-recorded play session and one VIT session (in a group setting) per week. Evaluation of the caregivers included assessments of PRF and wellbeing, child assessment included parent-ratings and clinician-ratings of symptomatology and general functioning. Results: Thirty participants were randomized; eligibility and recruitment rate were 70.6% and 83.3% respectively. Compliance-to-intervention rate was 85% in the VIT group and 90% in the control group. All participants completed entry evaluation and 90% at 3 months follow-up. The intervention was acceptable to participants and feasible for therapists to deliver. Outcome data must be treated with caution due to the small numbers involved, yet indicate that the VIT may have a positive effect in improving parental and child mental health outcomes.Conclusions: VIT for primary caregivers of child inpatient children was feasible to deliver and acceptable for participants, therapist and the staff unit involved, there is sufficient evidence to undertake a full-scale effectiveness RCT.Trial registration: ClinicalTrials.gov, NCT03374904. Registered on 14 December 2017
Renal Denervation Ameliorates Cardiomyocyte Apoptosis in Myocardial Ischemia-reperfusion Injury
Authors: Zhao Zheng , Li Faquan , Lu Chengzhi , Jiang Yiyao 
Published: January 2021

Abstract
Background: Myocardial ischemia-reperfusion injury (I/R) has been improved with drugs and effective reperfusion, but it still cannot be prevented. We compared the effects of renal denervation (RDN) and angiotensin receptor neprilysin inhibitors (ARNIs) on cardiomyocyte apoptosis after I/R to explore whether RDN can reduce cardiomyocyte apoptosis by improving endoplasmic reticulum stress.Methods: Sixty male specific pathogen free (SPF) Wister rats were randomly divided into six groups.(n=6):(a) normal; (b) sham; (c) I/R; (d) RDN; (e) I/R+RDN and (f) I/R+ARNI. We established the I/R rat model by ligating the left anterior descending artery. Rats were exposed tThe renal artery was exposed and then smeared phenol on the vessel for chemical ablation. The I/R+ARNI group received ARNIs for 2 weeks until killed. We also collected the cardiac tissues and blood serum to determine I/R-related indicators and analyze the potential mechanisms involved.Results: The levels of Norepinephrine (NE), Ang II, and aldosterone (ALD)increased significantly in the I/R group but decreased significantly after RDN and ARNI intervention. The expression of Bax, caspase-3, CHOP, PERK, and ATF4 protein was significantly increased in the I/R group, which compared to other groups, and the level of CHOP, PERK, and ATF4 gene expression increased. After RDN intervention, these expression levels recovered to varying degrees. Conclusion: Our results provide new evidence that RDN ameliorates cardiomyocyte apoptosis in I/R. The effect of RDN may be associated with regulating the endoplasmic reticulum stress PERK/ATF4 signaling pathway.