bluuebunny/crossref_metadata_2025_split huggingface dataset:
Training basic numerical skills in children with Down syndrome using the computerized game “The Number Race”
Authors: Sella Francesco , Onnivello Sara , Lunardon Maristella , Lanfranchi Silvia , Zorzi Marco 
Published: January 2021

AbstractIndividuals with Down syndrome (DS) present reduced basic numerical skills, which have a negative impact on everyday numeracy and mathematical learning. Here, we evaluated the efficacy of the adaptive (non-commercial) computerized game “The Number Race” in improving basic numerical skills in children with DS. The experimental group (EG; N = 30, Mage-in-months118, range 70–149) completed a training playing with “The Number Race”, whereas children in the control group (CG; N = 31, Mage-in-months138, range 76–207) worked with software aiming at improving their reading skills. The training lasted 10 weeks with two weekly sessions of 20–30 min each. We assessed both groups’ numerical and reading skills before and immediately after the end of the training, as well as at a 3-months follow-up. We found weak evidence for post-training groups differences in terms of overall numeracy score. However, the EG displayed substantial improvements in specific numerical skills and in mental calculation, which were maintained over time, and no improvement in reading. Conversely, the CG showed improvements in their reading skills as well as in number skills but to a lesser extent compared to the EG. Overall, “The Number Race” appears as a suitable tool to improve some aspects of numeracy in DS.
Laser-sound: optoacoustic transduction from digital audio streams
Authors: Kaleris Konstantinos , Stelzner Björn , Hatziantoniou Panagiotis , Trimis Dimosthenis , Mourjopoulos John 
Published: January 2021

AbstractThis work presents a novel laser-based optoacoustic transducer capable of reproducing controlled and continuous sound of arbitrary complexity in the air or on solid targets. Light-to-sound transduction is achieved via laser-induced breakdown, leading to the formation of plasma acoustic sources in any desired spatial location. The acoustic signal is encoded into pulse streams via a discrete-time audio modulation and is reproduced by fast consecutive excitation of the target medium with appropriately modulated laser pulses. This results in the signal being directly reconstructed at the desired location of the target medium without the need for a receiver or demodulation device. In this work, the principles and evaluation results of such a novel laser-sound prototype system are presented. The performance of the prototype is evaluated by systematic experimental measurements of audio test signals, from which the basic acoustical response is derived. Moreover, a generic computational model is presented that allows for the simulation of laser-sound reproduction of 1-bit or multibit audio streams. The model evaluations are validated by comparison with the acoustic measurements, whereby a good agreement is found. Finally, the computational model is used to simulate an ideal optoacoustic transducer based on the specifications of state-of-the-art commercially available lasers.
Effects of a commercially formulated glyphosate solutions at recommended concentrations on honeybee (Apis mellifera L.) behaviours
Authors: Luo Qi-Hua , Gao Jing , Guo Yi , Liu Chang , Ma Yu-Zhen , Zhou Zhi-Yong , Dai Ping-Li , Hou Chun-Sheng , Wu Yan-Yan , Diao Qing-Yun 
Published: January 2021

AbstractGlyphosate, the active ingredient of the most widely used commercial herbicide formulation, is extensively used and produced in China. Previous studies have reported sublethal effects of glyphosate on honeybees. However, the effects of commercially formulated glyphosate (CFG) at the recommended concentration (RC) on the chronic toxicity of honeybees, especially on their behaviours, remain unknown. In this study, a series of behavioural experiments were conducted to investigate the effects of CFG on honeybees. The results showed that there was a significant decline in water responsiveness at 1/2 × , 1 × and 2 × the RC after 3 h of exposure to CFG for 11 days. The CFG significantly reduced sucrose responsiveness at 1/2 × and 1 × the RC. In addition, CFG significantly affected olfactory learning ability at 1/2 × , 1 × , and 2 × the RC and negatively affected memory ability at 1/2 × and 1 × the RC. The climbing ability of honeybees also significantly decreased at 1/2 × , 1 × and 2 × the RC. Our findings indicated that, after they were chronically exposed to CFG at the RC, honeybees exhibited behavioural changes. These results provide a theoretical basis for regulating field applications of CFG, which is necessary for establishing an early warning and notification system and for protecting honeybees.
Fluctuating environmental light limits number of surfaces visually recognizable by colour
Authors: Foster David H. 
Published: January 2021

AbstractSmall changes in daylight in the environment can produce large changes in reflected light, even over short intervals of time. Do these changes limit the visual recognition of surfaces by their colour? To address this question, information-theoretic methods were used to estimate computationally the maximum number of surfaces in a sample that can be identified as the same after an interval. Scene data were taken from successive hyperspectral radiance images. With no illumination change, the average number of surfaces distinguishable by colour was of the order of 10,000. But with an illumination change, the average number still identifiable declined rapidly with change duration. In one condition, the number after two minutes was around 600, after 10 min around 200, and after an hour around 70. These limits on identification are much lower than with spectral changes in daylight. No recoding of the colour signal is likely to recover surface identity lost in this uncertain environment.
Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
Authors: Mahadevan Gomathi , Valiyaveettil Suresh 
Published: January 2021

AbstractMicroplastic and nanoplastic particles are prevalent in the environment and are beginning to enter the living system through multiple channels. Currently, little is known about the impact of plastic nanoparticles in living organisms. In order to investigate the health impact of micro- and nanoparticles of common polymers in a systematic way, luminescent plastic nanoparticles from two common polymers, polyvinyl chloride (PVC) and poly (methyl methacrylate) (PMMA) with relatively narrow size distribution are prepared using a nanoprecipitation method. As a model system, BHK-21 cells were exposed to polymer nanoparticles to understand the mode of uptake, internalization and biochemical changes inside the cells. The cellular effects of the nanoparticles were evaluated by monitoring the changes in cell viability, cell morphology, concentrations of reactive oxygen species (ROS), adenine triphosphate (ATP) and lactate dehydrogenase at different concentrations of the nanoparticles and time of exposure. PVC and PMMA nanoparticles induced a reduction in the cell viability along with a reduction of ATP and increase of ROS concentrations in a dose- and time-dependent manner. The plastic nanoparticles are internalized into the cell via endocytosis, as confirmed by Dynasore inhibition assay and colocalization with latex beads. Our findings suggest that plastic nanoparticle internalization could perturb cellular physiology and affect cell survival under laboratory conditions.
Peripheral-central interplay for fatiguing unresisted repetitive movements: a study using muscle ischaemia and M1 neuromodulation
Authors: Madinabeitia-Mancebo Elena , Madrid Antonio , Oliviero Antonio , Cudeiro Javier , Arias Pablo 
Published: January 2021

AbstractMaximal-rate rhythmic repetitive movements cannot be sustained for very long, even if unresisted. Peripheral and central mechanisms of fatigue, such as the slowing of muscle relaxation and an increase in M1-GABAb inhibition, act alongside the reduction of maximal execution rates. However, maximal muscle force appears unaffected, and it is unknown whether the increased excitability of M1 GABAergic interneurons is an adaptation to the waning of muscle contractility in these movements. Here, we observed increased M1 GABAb inhibition at the end of 30 s of a maximal-rate finger-tapping (FT) task that caused fatigue and muscle slowdown in a sample of 19 healthy participants. The former recovered a few seconds after FT ended, regardless of whether muscle ischaemia was used to keep the muscle slowed down. Therefore, the increased excitability of M1-GABAb circuits does not appear to be mediated by afferent feedback from the muscle. In the same subjects, continuous (inhibitory) and intermittent (excitatory) theta-burst stimulation (TBS) was used to modulate M1 excitability and to understand the underlying central mechanisms within the motor cortex. The effect produced by TBS on M1 excitability did not affect FT performance. We conclude that fatigue during brief, maximal-rate unresisted repetitive movements has supraspinal components, with origins upstream of the motor cortex.
Functional disruption of cortical cingulate activity attenuates visceral hypersensitivity and anxiety induced by acute experimental colitis
Authors: Brenner Lukas , Zerlin Leah , Tan Linette Liqi 
Published: January 2021

AbstractVisceral pain is a highly complex experience and is the most common pathological feature in patients suffering from inflammatory gastrointestinal disorders. Whilst it is increasingly recognized that aberrant neural processing within the gut-brain axis plays a key role in development of neurological symptoms, the underlying mechanisms remain largely unknown. Here, we investigated the cortical activation patterns and effects of non-invasive chemogenetic suppression of cortical activity on visceral hypersensitivity and anxiety-related phenotypes in a well-characterized mouse model of acute colitis induced by dextran sulfate sodium (DSS). We found that within the widespread cortical network, the mid-cingulate cortex (MCC) was consistently highly activated in response to innocuous and noxious mechanical stimulation of the colon. Furthermore, during acute experimental colitis, impairing the activity of the MCC successfully alleviated visceral hypersensitivity, anxiety-like behaviors and visceromotor responses to colorectal distensions (CRDs) via downregulating the excitability of the posterior insula (PI), somatosensory and the rostral anterior cingulate cortices (rACC), but not the prefrontal or anterior insula cortices. These results provide a mechanistic insight into the central cortical circuits underlying painful visceral manifestations and implicate MCC plasticity as a putative target in cingulate-mediated therapies for bowel disorders.
Detection and characterization of ESBL-producing Enterobacteriaceae from the gut of subsistence farmers, their livestock, and the surrounding environment in rural Nepal
Authors: Subramanya Supram Hosuru , Bairy Indira , Metok Yang , Baral Bharat Prasad , Gautam Dipendra , Nayak Niranjan 
Published: January 2021

AbstractThe increasing trend of gut colonization by extended-spectrum β-lactamase (ESBL) producing Enterobacterales has been observed in conventional farm animals and their owners. Still, such colonization among domesticated organically fed livestock has not been well studied. This study aimed to determine the gut colonization rate of ESBL-producing Enterobacteriaceae and carbapenemase-producing Enterobacteriaceae (CPE) among rural subsistence farming communities of the Kaski district in Nepal. Rectal swabs collected by systematic random sampling from 128 households of subsistence farming communities were screened for ESBL-producing Enterobacteriaceae and CPE by phenotypic and molecular methods. A total of 357 (57%) ESBL-producing Enterobacteriaceae isolates were obtained from 626 specimens, which included 97 ESBL-producing Enterobacteriaceae (75.8%) from 128 adult humans, 101 (79.5%) from 127 of their children, 51 (47.7%) from 107 cattle, 26 (51%) from 51 goats, 30 (34.9%) from 86 poultry and 52 (42%) from 127 environmental samples. No CPE was isolated from any of the samples. blaCTX-M-15 was the most predominant gene found in animal (86.8%) and human (80.5%) isolates. Out of 308 Escherichia coli isolates, 16 human and two poultry isolates were positive for ST131 and were of clade C. Among non-cephalosporin antibiotics, the resistance rates were observed slightly higher in tetracycline and ciprofloxacin among all study subjects. This is the first one-health study in Nepal, demonstrating the high rate of CTX-M-15 type ESBL-producing Enterobacteriaceae among gut flora of subsistence-based farming communities. Gut colonization by E. coli ST131 clade C among healthy farmers and poultry birds is a consequential public health concern.
Structural insights into vesicle amine transport-1 (VAT-1) as a member of the NADPH-dependent quinone oxidoreductase family
Authors: Kim Sun-Yong , Mori Tomoyuki , Chek Min Fey , Furuya Shunji , Matsumoto Ken , Yajima Taisei , Ogura Toshihiko , Hakoshima Toshio 
Published: January 2021

AbstractVesicle amine transport protein-1 (VAT-1) has been implicated in the regulation of vesicular transport, mitochondrial fusion, phospholipid transport and cell migration, and is a potential target of anticancer drugs. Little is known about the molecular function of VAT-1. The amino acid sequence indicates that VAT-1 belongs to the quinone oxidoreductase subfamily, suggesting that VAT-1 may possess enzymatic activity in unknown redox processes. To clarify the molecular function of VAT-1, we determined the three-dimensional structure of human VAT-1 in the free state at 2.3 Å resolution and found that VAT-1 forms a dimer with the conserved NADPH-binding cleft on each protomer. We also determined the structure of VAT-1 in the NADP-bound state at 2.6 Å resolution and found that NADP binds the binding cleft to create a putative active site with the nicotine ring. Substrate screening suggested that VAT-1 possesses oxidoreductase activity against quinones such as 1,2-naphthoquinone and 9,10-phenanthrenequinone.
The RNA-binding protein LARP1 is dispensable for pancreatic β-cell function and mass
Authors: Werneck-de-Castro Joao Pedro , Peçanha Flavia Leticia Martins , Silvestre Diego Henrique , Bernal-Mizrachi Ernesto 
Published: January 2021

AbstractMechanistic target of rapamycin complex 1 (mTORC1) deficiency or chronic hyperactivation in pancreatic β-cells leads to diabetes. mTORC1 complexes with La-related protein 1 (LARP1) to specifically regulate the expression of 5′ terminal oligopyrimidine tract (5′TOP) mRNAs which encode proteins of the translation machinery and ribosome biogenesis. Here we show that LARP1 is the most expressed LARP in mouse islets and human β-cells, being 2–4-fold more abundant than LARP1B, a member of the family that also interacts with mTORC1. Interestingly, β-cells from diabetic patients have higher LARP1 and LARP1B expression. However, specific deletion of Larp1 gene in β-cells (β-Larp1KO mice) did not impair insulin secretion and glucose metabolism in male and female mice. High fat or high branched-chain amino acid (BCAA) diets did not disturb glucose homeostasis compared to control littermates up to 8 weeks; BCAA diet slightly impaired glucose tolerance in the β-Larp1KO mice at 16 weeks. However, no differences in plasma insulin levels, non-fasting glycemia and β-cell mass were observed in the β-Larp1KO mice. In conclusion, LARP1 is the most abundant LARP in mouse islets and human β-cells, and it is upregulated in diabetic subjects. However, genetically disruption of Larp1 gene did not impact glucose homeostasis in basal and diabetogenic conditions, suggesting no major role for LARP1 in β-cells.
Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
Authors: Naruse Mie , Ochiai Masako , Sekine Shigeki , Taniguchi Hirokazu , Yoshida Teruhiko , Ichikawa Hitoshi , Sakamoto Hiromi , Kubo Takashi , Matsumoto Kenji , Ochiai Atsushi , Imai Toshio 
Published: January 2021

AbstractOrganoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this study, we established organoids and paired cancer-associated fibroblasts (CAFs) from surgical specimens of colorectal carcinomas (CRCs), and evaluated gene expression profiles in organoids with and without co-culture with CAFs to assess interactions between tumor cells and CAFs in tumor tissues. We found that the expression levels of several genes, which are highly expressed in original CRC tissues, were downregulated in organoids but re-expressed in organoids by co-culturing with CAFs. They comprised immune response- and external stimulus-related genes, e.g., REG family and dual oxidases (DUOXs), which are known to have malignant functions, leading tumor cells to proliferative and/or anti-apoptotic states and drug resistant phenotypes. In addition, the degree of differential induction of REG1 and DUOX2 in the co-culture system varied depending on CAFs from each CRC case. In conclusion, the co-culture system of CRC organoids with paired CAFs was able to partially reproduce the tumor microenvironment.
Bicuspid aortic valve morphology and aortic valvular outflow jets: an experimental analysis using an MRI-compatible pulsatile flow circulation system
Authors: Hattori Kaoru , Nakama Natsuki , Takada Jumpei , Nishimura Gohki , Moriwaki Ryo , Kawasaki Eita , Nagao Michinobu , Goto Yasuhiro , Niinami Hiroshi , Iwasaki Kiyotaka 
Published: January 2021

AbstractThe characteristics of aortic valvular outflow jet affect aortopathy in the bicuspid aortic valve (BAV). This study aimed to elucidate the effects of BAV morphology on the aortic valvular outflow jets. Morphotype-specific valve-devising apparatuses were developed to create aortic valve models. A magnetic resonance imaging-compatible pulsatile flow circulation system was developed to quantify the outflow jet. The eccentricity and circulation values of the peak systolic jet were compared among tricuspid aortic valve (TAV), three asymmetric BAVs, and two symmetric BAVs. The results showed mean aortic flow and leakage did not differ among the five BAVs (six samples, each). Asymmetric BAVs demonstrated the eccentric outflow jets directed to the aortic wall facing the smaller leaflets. In the asymmetric BAV with the smaller leaflet facing the right-anterior, left-posterior, and left-anterior quadrants of the aorta, the outflow jets exclusively impinged on the outer curvature of the ascending aorta, proximal arch, and the supra-valvular aortic wall, respectively. Symmetric BAVs demonstrated mildly eccentric outflow jets that did not impinge on the aortic wall. The circulation values at peak systole increased in asymmetric BAVs. The bicuspid symmetry and the position of smaller leaflet were determinant factors of the characteristics of aortic valvular outflow jet.
SARS-CoV-2-Diagnostik – was ist wirklich sinnvoll?
Authors: Floß Niklas , Rassaf Tienush , Rohn Hana , Witzke Oliver 
Published: January 2021

ZusammenfassungDen Goldstandard zur Diagnose einer Infektion mit SARS-CoV-2 stellt aktuell (Stand November 2020) die PCR-Untersuchung dar. Diese ist jedoch vor allem zeitintensiv. In Zukunft könnten sog. Antigentests schnell und patientennah als Point-of-Care-Methode durchgeführt werden, um die Diagnosestellung einer SARS-CoV-2 Infektion zu erleichtern. Veränderungen serologischer Biomarker (wie D-Dimere, Troponin T, CRP) können helfen, Patienten mit einem Risiko für einen schweren COVID-19-Verlauf zu identifizieren. Ein routinemäßiges Screening-CT-Thorax zur Identifizierung einer COVID-19-Pneumonie wird derzeit von den meisten radiologischen Gesellschaften nicht empfohlen. Dennoch nimmt die Zahl der durchgeführten CT-Untersuchungen kontinuierlich zu, und die radiologische Diagnostik nimmt bei an COVID-19 erkrankten Patienten mit pulmonalen Symptomen einen immer wichtigeren Stellenwert in der klinischen Erfassung der Schwere der Lungenbeteiligung ein.
A Comparative Analysis of Ophthalmology Resident Physician Performance Based on Use of Parental Leave
Authors: Fliotsos Michael J. , Zafar Sidra , Woreta Fasika A. , Ugoh Peter M. , Srikumaran Divya 
Published: January 2021

Abstract
Background Taking parental leave during ophthalmology residency may be perceived to negatively affect resident surgical volume and educational outcomes. However, limited data exist on whether taking parental leave is associated with objective measures of resident performance. The objective of the present study was to determine the association between taking parental leave and key measures of resident performance.
Methods Educational records of ophthalmology resident physicians who graduated from 2015 to 2019 at the Johns Hopkins University School of Medicine Wilmer Eye Institute were reviewed. Measures of resident performance assessed were Ophthalmic Knowledge Assessment Program scores, number of publications during residency, Accreditation Council for Graduate Medical Education milestones scores, and surgical volumes. These outcomes were compared for residents who took parental leave compared with their peers who did not take parental leave.
Results Twenty-five residents were included in the study. Four female residents (16%) took parental leave 8 weeks in duration. There were no significant differences between residents who did and did not take parental leave when considering mean Ophthalmic Knowledge Assessment Program scores (p = 0.27), number of publications during residency (p = 0.19), milestone scores (p-value range 0.09–0.40), and surgical volume by subspecialty category (p-value range 0.11–0.45).
Conclusion Parental leave did not negatively influence any of the studied measures of resident performance.