bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and application of TaqMan probe‐based quantitative PCR assays for the detection of tilapia parvovirus
Authors: Yamkasem Jidapa , Tattiyapong Puntanat , Surachetpong Win 
Published: December 2021

AbstractTilapia parvovirus (TiPV) is a novel parvovirus associated with high mortality in Nile tilapia and red hybrid tilapia, leading to severe economic losses for tilapia aquaculture. It is critical to develop a sensitive and accurate assay to detect TiPV in fish tissues. In this study, new TaqMan probe‐based quantitative PCR (qPCR) assays targeting the non‐structural (NS) and viral protein (VP) genes of TiPV were developed. The standard curves of the assays were 95.64%–98.96% over a wide linear range of 109–101 copies of the corresponding standard DNA per reaction. The intra‐ and inter‐assay coefficients of variation were in the ranges 0.54%–2.50% and 0.13%–1.17%, respectively, which suggests good repeatability and reproducibility. The detection limit of the TaqMan TiPV assays was 10 copies/µl. The application of the TaqMan qPCR assays to field samples revealed that they had comparable sensitivity to a previously developed SYBR Green qPCR, but more sensitive than the conventional PCR. No cross‐reactivity of the TaqMan TiPV assays was found with the samples infected with other viruses and bacteria. Overall, the assays offered high sensitivity and specificity in the detection of low concentrations of TiPV DNA in infected tilapia samples. These new TaqMan qPCR assays could provide a valuable diagnostic tool for the reliable and specific detection of TiPV in experimental and field samples.
Morphological abnormalities in seven American round ray specimens: A review of America's batomorph anomalies
Authors: Ehemann Nicolás Roberto , García‐Rodríguez Francisco Javier , De La Cruz‐Agüero José 
Published: December 2021

AbstractAlthough morphological abnormalities in several rays and skate species around the American continents have frequently mentioned, their numbers are unknown. The present work record morphological abnormalities in four Urotrygonidae species. Two anophthalmic specimens were detected (Urotrygon microphthalmum andUrobatis halleri). Two individuals lacked caudal fins (Urobatis maculatusandUrotrygon chilensis). Two round rays showed incomplete fusion of the pectoral fin to the head (U. microphthalmumandU. chilensis). Vertebral compression and fusion were found in a 6‐year‐old femaleUrotrygon rogersi. In addition, 118 abnormal batomorph specimens were gathered from the available bibliography, spanning the last six decades (1959–2021).Amblyraja doellojuradoiwas the species with the highest number of abnormalities (18). The most common anomaly was an incomplete fusion of the pectoral fin with the head. Since 2010, at least 30 anomalous batomorphs have been recorded every 5 years. Sixty‐nine abnormal specimens occurred in the Northern Hemisphere (1.00–60.00 N). The Cortezian (Pacific) and Southeastern Brazil (Atlantic) marine ecoregions stood out with the highest number of these specimens. Mexico recorded 58 anomalous specimens, followed by Brazil (n = 36). Biological, abiotic and anthropogenic factors are probably the leading causes. However, additional studies are necessary to elucidate these speculations.
Factors associated with depression and anxiety among caregivers of patients with atrial fibrillation
Authors: Li Sitong , Li Qifan , Jiang Chao , Chen Xuan , Lai Yiwei , He Liu , Cui Feihuan , Wu Jiahui , Hu Rong , Jia Changqi , Feng Li , Sang Caihua , Tang Ribo , Long Deyong , Du Xin , Dong Jianzeng , Ma Changsheng 
Published: December 2021

AbstractAims and objectivesTo assess the prevalence and associated factors of depression and anxiety among caregivers of patients with atrial fibrillation.BackgroundDepression and anxiety are common in caregivers of patients with cardiovascular diseases, including heart failure and coronary artery disease. However, studies about depression and anxiety among caregivers of patients with atrial fibrillation are limited.DesignCross‐sectional study.MethodsWe enrolled 465 dyads of patients with atrial fibrillation and their primary family caregivers from Beijing Anzhen Hospital between September 2020 and March 2021. The patient–caregiver dyads were excluded if primary family caregivers had previous mental disorders before the patient diagnosis of atrial fibrillation. Depression and anxiety of patients and caregivers were measured by Patient Health Questionnaire‐9 and Generalized Anxiety Disorder‐7 scale. Multivariate logistic regression analysis was used to assess factors associated with depression and anxiety of caregivers. STROBE guidelines were followed to report this study.ResultsThe prevalence of caregiver depression (Patient Health Questionnaire‐9 score ≥5) and anxiety (Generalized Anxiety Disorder‐7 score ≥5) was 14.0% and 13.5% respectively. Caregiver number of comorbidities ≥2 and patient depression were significantly associated with caregiver depression. Caregiver age ≥65 years, caregiver female sex and patient anxiety were predictors of caregiver anxiety.ConclusionsDepression and anxiety are common in caregivers of patients with AF. Better management of caregiver mental problems and associated factors may benefit both patients and caregivers.Relevance to clinical practiceClinicians and nurses should pay more attention to depression and anxiety in caregivers of patients with atrial fibrillation, and provide support to caregivers in most need.
One talk at a time: Developing an ethnic‐racial socialization intervention for Black, Latinx, and Asian American families
Authors: Stein Gabriela Livas , Coard Stephanie Irby , Gonzalez Laura M. , Kiang Lisa , Sircar Joseph K. 
Published: December 2021

AbstractEthnic‐racial socialization (ERS) can promote positive outcomes in minoritized youth, but parents often face challenges in effectively engaging in these conversations. We describe the development of a video‐based online parent intervention program aimed at improving parental motivation, efficacy, and skills in having ERS conversations. The program focuses on balancing cultural socialization and preparation for bias messages and integrates themes of coping with future discrimination with the goal of promoting adjustment in youth. The video‐based program (One Talk at A Time: OTAAT) consists of 11 short videos designed to bolster parental efficacy and skills via motivational, didactic modules, and social modeling. We describe our intervention (specifically designed for Black, Latinx, and Asian American families), a sample of parents (N = 15) who interfaced with the intervention, and preliminary evidence of its impact via a pre‐post design and program evaluation interviews. Discussion centers on implications of our approach for future prevention efforts.
#BlackGirlMagic: Using multiple data sources to learn about Black adolescent girls’ identities, intersectionality, and media socialization
Authors: Rogers Leoandra Onnie , Butler Barnes Sheretta , Sahaguian Lily , Padilla Dayanara , Minor Imani 
Published: December 2021

AbstractA robust literature shows that (parental) ethnic‐racial socialization (ERS) influences Black youths’ identity development. Other sources of socialization, like media, are acknowledged but scarcely represented in empirical studies. In this study, we conceptualize #BlackGirlMagic (#BGM), a social media hashtag, as a media‐based ERS message and examine its association with identity outcomes among Black adolescent girls (N = 63; Mage = 16.17). Analyzing qualitative and quantitative data, we show that Black girls interpret #BGM as a message of: (a) Affirmation; (b) Societal Oppression; (c) Collectiveness; and (d) Being Seen/Heard. Furthermore, girls’ interpretations of #BGM were significantly associated with racial identity measures and the content of their racial and gender identity interviews. Implications for socialization and identity research and the study of Black girls’ development are discussed.
Effects of genotype, parity, season and their interactions on milk yield in crossbred dairy cattle
Authors: Barua Karabi , Akter Nasima , Alam Mahabub , Bari Md. Saiful , Sultan Md. Nahid , Islam Shilpi , Hossain Md. Emran 
Published: December 2021

AbstractThe crossbred dairy cattle (CDC) have been gaining popularity in the tropical countries for their increased milk yield within a short period of time because of heterogenic additive gene action. Hence, we aimed to investigate whether genotype, parity, season and their interactions had any effect on average daily milk yield (ADMY) of the CDC in a dairy farm at Chattogram district, Bangladesh, for a period of 3 years from January 2016 to December 2019. Total 16,425 retrospective lactation records of 150 multiparous Sahiwal × Friesian1 (Sahiwal = 50%, HF = 50%), Local × Friesian1 (Local = 50%, HF = 50%) and Local × Friesian2 (Local = 25%, HF = 75%) CDC from the first to the third parities (50 for each parity) were collected from the farm records. The generalized linear model and principal component analysis identified substantial impacts of genotype, parity, season and their interactions on ADMY of the CDC. The herd level least squared ADMY was 11.22 ± 0.04 kg/days on a 305‐days typical lactation period. The Sahiwal × Friesian1 CDC produced 7.2% and 5.5% more milk than the Local × Friesian1 and Local × Friesian2 respectively. The CDC produced maximum milk in the second parity, which was 7.8% and 0.34% more than the first and third parities. Similarly, the highest ADMY was recorded in the spring, which was 10.8%, 7.3% and 6.6% more than the fall, summer and winter respectively. It was concluded that the Sahiwal × Friesian1 crossbred produced maximum milk in the spring season at the second parity while other determinants remained constant. The changing patterns of milk yield in different genotype, parity and seasons provided scientific evidence for improving feeding strategy to optimize herd level milk yield of CDC in the commercial dairy farms under tropical perspective.
Psychological Resilience After Cancer via Religion/Spirituality: Spiritual Capital Through a Life Course Lens
Authors: Upenieks Laura 
Published: December 2021

AbstractReligious beliefs and practices are thought to help people confront problems that push the limits of human life. Integrating the life course perspective, we assess whether the accumulation of religiosity (“spiritual capital”) between childhood and adulthood had any bearing on its ability to cushion the mental health insults of a cancer diagnosis, factoring in age‐at‐diagnosis. Using two waves of data from National Survey of Midlife Development in the United States (N = 1431), results suggest that stable high religious importance between childhood and adulthood weakened the deleterious mental health consequences of a cancer diagnosis. For individuals under the age of 45, the relationship between a cancer diagnosis and psychological distress was considerably weaker for those reporting stably high or increasing religious importance between childhood and adulthood. We discuss the implications of our results for research at the intersection religion and the life course perspective.
Improvements of dried noodles qualities using two‐stage resting: Explanation from macro and micro perspectives
Authors: Li Xue , Qi Yajing , Hassane Hamadou Alkassoumi , Zhang Jiyao , Guo Qin , Xu Bin 
Published: December 2021

AbstractThe purpose of this study was to elucidate the effects of two‐stage resting (dough crumbs and dough sheets resting) on the quality of dried noodles. An improvement mechanism was explored according to the texture of dough sheets, gluten network, and microstructure of cooked noodles. After two‐stage resting, the extensibility of dough sheets, the contents of glutenin macro polymer (GMP), and disulfide bonds (S‐S) increased significantly (p < .05), whereas the content of sulphydryl group (‐SH) significantly declined. From the perspective of microstructure, the average length and width of dough sheets protein experienced a significant reduction (p < .05); however, the branching rate, the number of protein lines, and the total length of the protein lines significantly increased (p < .05). Furthermore, the average network line length and width of cooked noodles were significantly reduced as the branching rate rose (p < .05); meanwhile, the number of holes significantly increased while the average area significantly decreased (p < .05). Overall, the two‐stage resting has improved the quality of dried noodles and contributed to a more uniform and compact gluten network distribution.
Health insurance rights and access to health care for trans people: The social construction of medical necessity
Authors: Kirkland Anna , Talesh Shauhin , Perone Angela K. 
Published: December 2021

AbstractHealth care rights for transgender and/or nonbinary people have dramatically expanded in recent years, including in insurance coverage for the treatments and procedures they need. Yet, trans people themselves still identify health insurance problems as a top priority for research and policy change because of significant difficulties gaining and using coverage. Wrangling over coverage determinations happens through multiple types of interactions, bureaucratic, interpersonal, and medical. When these interactions become difficult, it is because key terms such as medical necessity are both powerful and indeterminate. This study examines how trans people and health care intermediaries navigate the health insurance process and contest the meaning of medical necessity in coverage determinations. These disputes constitute the ground-level reality for instantiating health care rights to gender affirming care. Relying on analysis of contract language and 32 interviews with people who sought gender-affirming care and allied professionals, we find that health insurance policy language, interpretation, and implementation often create disadvantages and barriers for trans people who attempt to access care. Our study highlights how the contested life of insurance policy terminology produces a reality for rights but also details the mechanisms through which insurance-mediated care is a socially contested and negotiated process
Hydrothermal alteration at the basalt‐hosted Vista Alegre impact structure, Brazil
Authors: Alsemgeest Jitse , Brouwer Fraukje M. , Auqué Luis F. , Hauser Natalia , Reimold Wolf Uwe 
Published: December 2021

AbstractHydrothermal systems provide a possible habitat for early life and are key targets in the quest for life outside Earth. In impact craters on Mars, hydrous minerals can represent products of impact‐generated hydrothermal systems (IGHS) or minerals already present in the crust and exposed during impact‐caused excavation. Because of its basaltic target rock, similar in composition to Martian crust, the Vista Alegre impact structure in Brazil is one of the very few analog structures that may reveal the origin of these minerals, if evidence of hydrothermal alteration is established. This work presents the results of a systematic search for evidence of hydrothermal alteration at the Vista Alegre impact structure. Four types of alteration were identified, all within a 2.5–3.0 km radius from the crater center: a zircon‐bearing melt veinlet, two sets of hydrothermal veins consisting predominantly of calcite and chabazite, and local alteration comprising saponite. Thermodynamic modeling suggests subsequent heating and cooling for each of the hydrothermal vein sets. Combined thermodynamic and spectrometric evidence indicates that development of a vigorous IGHS is unlikely. If similar processes occur on Mars, hydrous minerals are more likely preimpact phases exposed by excavation, rather than being formed through an IGHS.
Could female steroids lead to osteonecrosis of the jaw?
Authors: Costa Samuel M. , Mota Viviani C. , Greco Bernardo B. , Bueno Sebastião C. , Amaral Marcio B. F. 
Published: December 2021

AbstractTibolone (TB) is a synthetic female steroid with an estrogenic hormonal profile that has been related to suppress bone resorption and neoformation. This drug usage has been increasing over the last few years, particularly among pre‐ and post‐menopause woman. This study presents the first known case of TB related osteonecrosis of the jaw and explains the relationship between the drug and the osteonecrosis pathogenesis. One hypothesis for the small number of cases relating TB and medication related osteonecrosis of the jaws is that the drug promotes a different impact on bone metabolism. In this cases it is mandatory a local trauma. On the illustrative case we present within this letter, the installation of implants was what triggered the alterations and the necrosis. All surgeons should be aware of these drugs, and all the patients in the group of menopausal conditions should be asked about the use of this medication.
Long‐term outcomes of mandibular reconstruction using non‐vascularised rib grafts following large ameloblastoma resection in Ethiopia
Authors: de Berker Henry T. , Honeyman Calum , Patel Vinod , Yonis Elyas , Eshete Mekonen , Martin Dominique , McGurk Mark 
Published: December 2021

AbstractAimAmeloblastomas are odontogenic tumours that often require radical excision and mandibular reconstruction. This is challenging in resource‐limited settings where access to microsurgery is often scarce or absent. Currently, some patients are left without reconstruction, and others with bulky metal plates prone to complications. This leads to disfigurement and poor functional outcomes. We aim to establish robust long‐term outcomes for non‐vascularised autologous rib grafts for patients in this setting.Materials and MethodsTwelve adult patients with large ameloblastomas underwent hemimandibulectomy and rib graft reconstruction during international surgical collaborations in Ethiopia between 2012 and 2015. A long‐term follow‐up was conducted in rural Ethiopia in February 2017.ResultsFollow‐up was possible for seven patients (58% of those treated). Mean post‐operative follow‐up time was 42.9 months (SD 11.7; range 24–60 months). Early complications were four superficial surgical site infections. Late complications were two cases of wire extrusion and one of keloid scarring. There were no graft failures. Patients reported reduced social stigma (p = 0.001) and pain (p = 0.03), excellent function and 100% satisfaction.ConclusionWhen situational factors preclude the use of microsurgery, bundle rib grafts offer a reliable alternative for total mandibular reconstruction for patients after ameloblastoma resection. Mild complications were experienced by most patients, but these were straightforward to manage and the rib grafts still took well, being surprisingly resistant to infection. Importantly, this technique is associated with long‐term improvements in social stigma and quality of life for patients.
Long‐Term Clinical Outcomes and Survivorship of Total Hip Arthroplasty for Pyogenic Arthritis: A Retrospective Cohort Study of 168 Hips
Authors: Zeng Wei‐Nan , Zeng Lin , Guo Qiang , Yu Qiu‐Ping , Wang Hao‐Yang , Luo Ze‐Yu , Pei Fu‐Xing , Zhou Zong‐Ke 
Published: December 2021

ObjectiveThe aim of the present study was to evaluate the 10‐year outcomes of cementless total hip arthroplasty (THA) in adult patients with the late sequelae of septic arthritis of the hip.MethodsWe followed 166 consecutive patients (168 hips) who underwent cementless THA between March 2001 and December 2011. There were 79 men and 87 women, with a mean age of 50.4 years (range 21–76 years) at the time of index THA, all of whom had hip osteoarthritis secondary to hip pyogenic infection. The average duration of follow up was 10.6 years (range 6.9–17.2 years). Preoperative and postoperative clinical ratings were evaluated, including the hip dysfunction and osteoarthritis outcome score (HOOS), the Harris hip score (HHS), range of motion, a 100‐point visual analog scale for hip pain, and the severity of limp and limb length discrepancy (LLD). The anteroposterior and lateral radiographs of the hip and full‐length view of the lower extremities were obtained to assess the position of the components, radiolucent lines, osteolysis, loosening of components, and heterotopic ossification. The intraoperative and postoperative complications were also recorded.ResultsThe mean HSS and hip pain score were 44.2 points (range 29–66 points) and 42.5 points (range 32–64 points), respectively, before the index surgery and significantly improved to 88.1 points (range 78–96 points) and 15.1 points (range 10–26 points), respectively, at final follow‐up examination. The HOOS and range of motion also improved significantly. The mean limb length discrepancy was reduced from 2.6 to 0.8 cm. The limp at last follow‐up examination was moderate in 3 cases because of hip osteoarthritis in the other limb, slight in 26, and absent in 137. A radiolucent line was observed in 12 hips (7.1%) around the acetabular or femoral components. A progressive radiolucent line around the undersized femoral stem in all zones was seen in 1 hip, resulting in aseptic loosening and breakage of the femoral component. There were 8 cases of intraoperative fracture, 7 cases of dislocation, and 7 cases of transient nerve palsy. Recurrence of infection occurred in 2 hips. Revision surgery was conducted in 2 hips because of isolated loosening of the acetabular cup and the femoral stem, respectively. Kaplan–Meier survival was 97% at 10 years, with revision for any reason with any component as an end‐point.ConclusionImproved surgical techniques and development of components with various sizes provided favorable results for cementless THA conducted for late sequelae of sepsis in these young and active patients. Although the incidence of complications was relatively high, the complications were treated successfully.