bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alcohol and Vapourized Nicotine Co-Exposure During Adolescence Contribute Differentially to Sex-Specific Behavioural Effects in Adulthood
Authors: Ruffolo Jessica , Frie Jude A. , Thorpe Hayley H.A. , Talhat M. Asfandyaar , Khokhar Jibran Y. 
Published: June 2021

ABSTRACTIntroductionCo-occurrence of e-cigarette use and alcohol consumption during adolescence is frequent. However, little is known about their long-lasting effects when combined. Here, we examined whether adolescent co-exposure to alcohol drinking and vapourized nicotine would impact reward- and cognition-related behaviours in adult male and female rats during adulthood.MethodsFour groups of male and female Sprague Dawley rats (n=8-11/group/sex) received either nicotine (JUUL 5% nicotine pods) or vehicle vapour daily between postnatal days 30-46, while having continuous voluntary access to ethanol and water during this time in a two-bottle preference design. Upon reaching adulthood, rats underwent behavioural testing utilizing Pavlovian conditioned approach testing, fear conditioning and a two-bottle alcohol preference test.ResultsA sex-dependent effect was found in the two-bottle preference test in adulthood such that females had a higher intake and preference for alcohol compared to males regardless of adolescent exposure; both male and female adult rats had greater alcohol preference compared to adolescents. Male rats exposed to vapourized nicotine with or without alcohol drinking during adolescence exhibited altered reward-related learning in adulthood, evidenced by enhanced levels of sign-tracking behaviour. Male rats that drank alcohol with or without nicotine vapour in adolescence showed deficits in associative fear learning and memory as adults. In contrast, these effects were not seen in female rats exposed to alcohol and nicotine vapour during adolescence.ConclusionsThe present study provides evidence that co-exposure to alcohol and vapourized nicotine during adolescence in male, but not female, rats produces longterm changes in reward- and cognition-related behaviours.ImplicationsThese findings enhance our understanding of the effects of alcohol drinking and nicotine vapour exposure in adolescence. Moreover, they highlight potential sex differences that exist in the response to alcohol and nicotine vapour, underscoring the need for follow-up studies elucidating the neurobiological mechanisms that drive these sex differences, as well as the long-term effects of alcohol and nicotine vapour use.
Comparative Structural Analyses of Selected Spike Protein-RBD Mutations in SARS-CoV-2 Lineages
Authors: Roy Urmi 
Published: June 2021

AbstractThe severity of the covid 19 has been observed throughout the world as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) had spread globally claiming more than 2 million lives and left a devastating impact on peoples’ life. Recently several virulent mutant strains of this virus, such as the B.1.1.7, B.1.351, and P1 lineages have emerged. These strains are predominantly observed in UK, South Africa and Brazil. Another extremely pathogenic B.1.617 lineage and its sub-lineages, first detected in India, are now affecting some countries at notably stronger spread-rates. This paper computationally examines the time-based structures of B.1.1.7, B.1.351, P1 lineages with selected spike protein mutations. Additionally, the mutations in the more recently found B.1.617 lineage and some of its sub-lineages are explored, and the implications for multiple point mutations of the spike protein’s receptor-binding domain (RBD) are described.
An F-actin mega-cable supports the migration of the sperm nucleus during the fertilization of the polarity-inverted central cell of Agave inaequidens
Authors: González-Gutiérrez Alejandra G. , Gutiérrez-Mora Antonia , Verdín Jorge , Rodríguez-Garay Benjamín 
Published: June 2021

AbstractAsparagaceae’s large embryo sacs display a central cell nucleus polarized toward the chalaza, which means the sperm nucleus that fuses it during double fertilization migrates a long distance before karyogamy. Because of the size and inverted polarity of the central cell in Asparagaceae, we hypothesize that the second fertilization process is supported by F-actin structures different from the short-range aster-like ones observed in Arabidopsis. Here, we analyzed the F-actin dynamics of Agave inaequidens, a typical Asparagaceae, before, during, and after central cell fertilization. Several parallel F-actin cables emerging from the nucleus within the central cell, enclosing the vacuole, and reaching the micropylar pole were observed. As fertilization progressed, a thick F-actin mega-cable traversing the vacuole appeared, connecting the central cell nucleus with the micropylar pole near the egg cell. This mega-cable wrapped the sperm nucleus in transit to fuse the central cell one. Once karyogamy finished, the mega-cable disassembled, but new F-actin structures formed during the endosperm development. These observations suggest that Asparagaceae, and probably other plant species with similar embryo sacs, evolved an F-actin machinery specifically adapted to support the migration of the fertilizing sperm nucleus within a large-sized and polarity-inverted central cell.
Dendrimer-like supramolecular assembly of proteins with a tunable size and valency through stepwise iterative growth
Authors: Bae Jin-Ho , Kim Hong-Sik , Kim Gijeong , Song Ji-Joon , Kim Hak-Sung 
Published: June 2021

AbstractThe assembly of proteins in a programmable manner provides insight into the underlying mechanisms of protein self-assembly in nature as well as the creation of novel functional nanomaterials for practical applications. Despite many advances, however, a rational protein assembly with an easy scalability in terms of size and valency remains a challenging task. Here, we present a simple bottom-up approach to the supramolecular protein assembly with a tunable size and valency in a programmable manner. The dendrimer-like protein assembly, called a “prodrimer,” was constructed using a total of three monomeric proteins: a core and two building-block proteins. The prodrimer generations were grown by a stepwise and alternate addition of a building block using two pairs of orthogonal protein-peptide interactions, leading to a higher-generation prodrimer with a mega-dalton size and multi-valency. The valency of the prodrimers at the periphery was tunable with the generation, enabling a single-step functionalization. A second-generation prodrimer functionalized with a target-specific protein binder showed a three-order of magnitude increase in binding affinity compared to a monomeric counterpart due to the avidity. The prodrimers functionalized with a targeting moiety and a cytotoxic protein cargo exhibited a highly enhanced cellular cytotoxicity, exemplifying their utility as a protein delivery platform. The present approach can be effectively used in the creation of protein architectures with new functions for biotechnological and medical applications.
How does the brain represent the semantic content of an image?
Authors: Xu Huawei , Liu Ming , Zhang Delong 
Published: June 2021

AbstractUsing deep neural networks (DNNs) as models to explore the biological brain is controversial, which is mainly due to the impenetrability of DNNs. Inspired by neural style transfer, we circumvented this problem by using deep features that were given a clear meaning—the representation of the semantic content of an image. Using encoding models and the representational similarity analysis, we quantitatively showed that the deep features which represented the semantic content of an image mainly predicted the activity of voxels in the early visual areas (V1, V2, and V3) and these features were essentially depictive but also propositional. This result is in line with the core viewpoint of the grounded cognition to some extent, which suggested that the representation of information in our brain is essentially depictive and can implement symbolic functions naturally.
SARS-CoV-2-Triggered Mast Cell Rapid Degranulation Induces Alveolar Epithelial Inflammation and Lung Injury
Authors: Wu Meng-Li , Liu Feng-Liang , Sun Jing , Li Xin , He Xiao-Yan , Zheng Hong-Yi , Pei Rong-Juan , Sun Hao , Zhou Yan-Heng , Yan Qi-Hong , Wu Cai-Xia , Chen Ling , Yu Guo-Ying , Chang Junbiao , Jin Xia , Zhao Jincun , Chen Xin-Wen , Zheng Yong-Tang , Wang Jian-Hua 
Published: June 2021

AbstractSARS-CoV-2 infection-induced hyper-inflammation links to the acute lung injury and COVID-19 severity. Identifying the primary mediators that initiate the uncontrolled hypercytokinemia is essential for treatments. Mast cells (MCs) are strategically located at the mucosa and beneficially or detrimentally regulate immune inflammations. Here we showed that SARS-CoV-2-triggeed MC degranulation initiated alveolar epithelial inflammation and lung injury. SARS-CoV-2 challenge induced MC degranulation in ACE-2 humanized mice and rhesus macaques, and a rapid MC degranulation could be recapitulated with Spike-RBD binding to ACE2 in cells; MC degranulation alterred various signaling pathways in alveolar epithelial cells, particularly, led to the production of pro-inflammatory factors and consequential disruption of tight junctions. Importantly, the administration of clinical MC stabilizers for blocking degranulation dampened SARS-CoV-2-induced production of pro-inflammatory factors and prevented lung injury. These findings uncover a novel mechanism for SARS-CoV-2 initiating lung inflammation, and suggest an off-label use of MC stabilizer as immunomodulators for COVID-19 treatments.Graphical abstractIn BriefSARS-CoV-2 triggers an immediate mast cell (MC) degranulation, which initiates the alveolar epithelial inflammation and disrupts the tight junction. MC stabilizers that block degranulation reduce virus-induced lung inflammation and injury.HighlightsThe binding of RBD of Spike protein of SARS-CoV-2-to ACE2 receptor protein triggers an immediate MC degranulationMC degranulation induces transcriptomic changes include an upregulated inflammatory signaling and a downregulated cell-junction signalingMC degranulation leads to alveolar epithelial inflammation and disruption of tight junctionsMC stabilizer that inhibits degranulation reduces SARS-CoV-2-induced lung inflammation and injury in vivo
Context coding in the mouse nucleus accumbens modulates motivationally-relevant information
Authors: Gmaz Jimmie M. , van der Meer Matthijs A. A. 
Published: June 2021

AbstractNeural activity in the nucleus accumbens (NAc) is thought to track fundamentally value-centric quantities such as current or future expected reward, reward prediction errors, the value of work, opportunity cost, and approach vigor. However, the NAc also contributes to flexible behavior in ways that are difficult to explain based on value signals alone, raising the question of if and how non-value signals are encoded in NAc. We recorded NAc neural ensembles while head-fixed mice performed a biconditional discrimination task where context-setting cues modulated the stimulus-outcome association of subsequently presented reward-predictive cues. We extracted single-unit and population-level correlates of task features, and found value-independent coding for the context cues. This context signal occupied a subspace orthogonal to outcome-predictive representations, and was predictive of subsequent behaviorally-relevant coding. Together, these findings support a circuit-level gating model for how the NAc contributes to behavioral flexibility and provide a novel population-level perspective from which to view NAc computations.
Machine Learning based Genome-Wide Association Studies for Uncovering QTL Underlying Soybean Yield and its Components
Authors: Yoosefzadeh-Najafabadi Mohsen , Torabi Sepideh , Torkamaneh Davoud , Tulpan Dan , Rajcan Istvan , Eskandari Milad 
Published: June 2021

AbstractGenome-wide association study (GWAS) is currently one of the important approaches for discovering quantitative trait loci (QTL) associated with traits of interest. However, insufficient statistical power is the limiting factor in current conventional GWAS methods for characterizing quantitative traits, especially in narrow genetic bases plants such as soybean. In this study, we evaluated the potential use of machine learning (ML) algorithms such as support vector machine (SVR) and random forest (RF) in GWAS, compared with two conventional methods of mixed linear models (MLM) and fixed and random model circulating probability unification (FarmCPU), for identifying QTL associated with soybean yield components. In this study, important soybean yield component traits, including the number of reproductive nodes (RNP), non-reproductive nodes (NRNP), total nodes (NP), and total pods (PP) per plant along with yield and maturity were assessed using 227 soybean genotypes evaluated across four environments. Our results indicated SVR-mediated GWAS outperformed RF, MLM and FarmCPU in discovering the most relevant QTL associated with the traits, supported by the functional annotation of candidate gene analyses. This study for the first time demonstrated the potential benefit of using sophisticated mathematical approaches such as ML algorithms in GWAS for identifying QTL suitable for genomic-based breeding programs.
Familial Danish dementia young Knock-in rats expressing humanized APP and human Aβ show impaired pre and postsynaptic glutamatergic transmission
Authors: Yin Tao , Yao Wen , Norris Kelly A. , D’Adamio Luciano 
Published: June 2021

ABSTRACTFamilial British and Danish dementia (FBD and FDD) are two neurodegenerative disorders caused by mutations in the Integral membrane protein 2B (ITM2b). BRI2, the protein encoded by ITM2b, tunes excitatory synaptic transmission at both pre- and post-synaptic terminus. Too, BRI2 interacts with and modulates proteolytic processing of Amyloid-β precursor Protein (APP), whose mutations cause familial forms of Alzheimer disease (FAD). To study pathogenic mechanism triggered by the Danish mutation we generated rats carrying the Danish mutation into the rat Itm2b gene (Itm2bD rats). Given the BRI2/APP interaction and the widely accepted relevance of human Aβ, a proteolytic product of APP, to AD, Itm2bD rats were engineered to express two humanized App alleles, to produce human Aβ. Here, we studied young Itm2bD rats to investigate early pathogenic changes. We found that peri-adolescent Itm2bD rats present subtle changes in human Aβ levels along with decreased spontaneous glutamate release and AMPAR-mediated responses but increased short-term synaptic facilitation in the hippocampal Schaeffer-collateral pathway. These changes are like those observed in adult mice producing rodent Aβ and carrying either the Danish or British mutations into the mouse Itm2b gene. Collectively, the data show that the pathogenic Danish mutation alters the physiological function of BRI2 at glutamatergic synapses; these functional alterations are detected across species and occur early in life. Future studies will be needed to determine whether this phenomenon represents an early pathogenic event in human dementia.
The impact of hybridity on PPP governance and related accountability mechanisms: the case of UK education PPPs
Authors: Stafford Anne , Stapleton Pamela 
Published: June 2021

PurposeContemporary organisational landscapes offer opportunities for hybrids to thrive. Public–private partnerships (PPPs) are one thriving hybrid form incorporating the use of resources and/or structures from both public and private sectors. The study examines the impact of such a hybrid structure on governance and accountability mechanisms in a context of institutional complexity.Design/methodology/approachThis study uses an approach that draws on institutional logics and hybridity to examine governance arrangements in the PPP policy created for the delivery of UK schools. Unusually, it employs a comparative case study of how four local governments implemented the policy. It draws on a framework developed by Polzeret al.(2017) to examine the level of engagement between multiple logics and hybrid structures and applies this to the delivery of governance and accountability for public money.FindingsThe Polzeret al.framework enables a study of how the nature of hybrids can vary in terms of their governance, ownership and control relations. The findings show how the relationships between levels of engagement of multiple logics and hybrid structures can impact on governance and accountability for public money. Layering and blending combinations led to increased adoption of private sector accountability structures, whilst a hybrid with parallel co-existence of community and market logics delivered a long-term governance structure.Research limitations/implicationsThe paper examines the operation of hybrids in a complex education PPP environment in only four local governments and therefore cannot provide representative answers across the population as a whole. However, given the considerable variation found across the four examples, the paper shows there can be significant differentiation in how multiple logics engage at different levels and in varying combinations even in the same hybrid setting. The paper focuses on capital investment implementation and its evaluation, so it is a limitation that the operational stage of PPP projects is not studied.Practical implicationsThe findings have political relevance because the two local government bodies with more robust combinations of multiple logics were more successful in getting funds and delivering schools in their geographical areas.Originality/valueThe study extends Polzeret al.'s (2017) research on hybridity by showing that there can be significant differentiation in how multiple logics engage at different levels and in varying combinations even in what was planned to be the same hybrid setting. It shows how in situations of institutional complexity certain combinations of logics lead to differentiation in governance and accountability, creating fragmented focus on the related public accountability structures. This matters because it becomes harder to hold government to account for public spending.
The development, implementation and challenges to water-saving practices in a water-stressed destination: a case study of the Songkran Festival, Thailand
Authors: Intason Montira , Coetzee Willem , Lee Craig 
Published: June 2021

PurposeThe contradiction between the purpose of the water-splashing tradition, the use of water in a water-stressed destination and overconsumption during a time of water shortage led the researchers to question the spirit of the Songkran Festival. It is important to keep the spirit of the festival alive without interrupting the livelihoods of the community and to critically engage with opportunities and challenges related to water-saving practices during the festival. Thus, this study aims to investigate the opportunities and challenges to responsible practices of water-saving at a cultural festival, using a case study of the water-splashing practice at the Songkran Festival in Thailand.Design/methodology/approachThis study used the cultural practice, namely, the water-splashing tradition at the Songkran Festival in Thailand, as a case study. The researchers conducted document analysis on local newspapers, the Bangkok Post specifically and participant observation at the Songkran Festival in the Khao San Road area in Bangkok, to gain first-hand insights to develop comprehensive results which answers the study’s objectives.FindingsThe key findings show three significant themes representing the opportunities for implementing sustainable water-saving practices; a call for a water-saving campaign, education on water saving and water-splashing restrictions. In terms of challenges to implementing water-saving practices, two key themes emerged; a lack of water stress and drought awareness and the hedonistic characteristic of water splashing. The study findings provide important implications to theory and practice for sustainable event management and provide considerations for event stakeholders to minimise water overuse in festivals.Originality/valueEnvironmental degradation is a key global issue that the United Nations addresses in the Sustainable Development Goals (SDGs) scheme (United Nations Development Program, 2016). According to Pereira et al. (2009), water scarcity can be caused by both human activities and natural causes. Factors such as unlimited water consumption, population growth and climate change are some of the contributing factors that not only affects resident's access to water but also on events hosted within communities. It is crucial that event managers critically re-think the way festivals are designed in water-stressed destinations.
“Dialysis and medical tourism. Investigating patients’ perceptions in Greece”
Authors: Traouda Vasiliki , Mpogiatzidis Panagiotis 
Published: June 2021

Purpose
The purpose of this paper is to explore the possibilities and the growth prospects of medical tourism in Greece, while particularly focusing on the dialysis process. Patients with end-stage renal failure have the right to any treatment deemed necessary during their journey to another city or country away from the place where their treatment normally takes place.

Design/methodology/approach
The survey has been conducted among 193 kidney disease patients that underwent extra-renal dialysis in two public and three private artificial kidney units in Thessaloniki. The study population consists of patients with end-stage chronic renal failure (CRF) that undergo dialysis. For the purpose of this study, quantitative research was carried out via a structured and anonymous questionnaire. The design and structure of the study were based upon questionnaires from two relevant questionnaires. The questionnaires were modified appropriately to respond to the subject in question. Finally, for the analysis of the data, the SPSS software has been used.

Findings
One of the most obvious results is that patients on dialysis treatment have limited both the frequency and the duration of their vacation. The 8.3% of patients that did not use to travel increased to 37.8%, whereas only 22.3% continue to travel 2–4 times a year. Patients that keep on going on vacation frequently claim that a break from the daily routine improves their quality of life. Most of the participants (up to 36.3%) claim they feel healthier, while 34.2% stated they experience a normal life when they escape their everyday life and travel far from home. The 69.9% claim that Greece has the potentials to be a popular medical tourism destination. However, a well-structured and government-financed assistance programme is absent. The processing of data has revealed a positive correlation between age and tourism behaviour. Additionally, according to the research results, younger patients tend to be better informed regarding medical tourism in comparison with older ones.

Social implications
Considering the patients to be potential travellers facilitates the development, realization and promotion of medical tourism.

Originality/value
This study attempts to investigate, for the first time, the tourism behaviour of chronic kidney disease patients. The study highlights a sensitive issue, patients’ right to treatment without geographical or distance-related obstacles.