bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variación espacio-temporal de macroinvertebrados acuáticos en la Lindosa, Guayana colombiana
Authors: Córdoba-Ariza Paula Gabriela , Rincón-Palau Katterine , Donato-R. Jhon Ch. , González-Trujillo Juan David 
Published: January 2021

Introducción: Describir los patrones de biodiversidad y los mecanismos que los subyacen es indispensable para afrontar las desenfrenadas presiones sobre los ecosistemas de agua dulce en el trópico. Esto es particularmente relevante en regiones escasamente estudiadas como el escudo guayanés colombiano Objetivo: Describir la estructura taxonómica y funcional de las comunidades de macroinvertebrados, y evaluar su variación a lo largo de escalas espaciales y temporales Métodos: Las comunidades de macroinvertebrados de cuatro ríos fueron muestreadas durante la época seca del 2017 y 2018. Adicionalmente, se evaluaron los patrones de diversidad dentro y entre los ríos y entre diferentes meses de muestreo en uno de los cursos hídricos. Los patrones de diversidad fueron evaluados mediante la comparación de los patrones entre y dentro de los ríos de la diversidad alfa (diferencias en la riqueza y abundancia de taxones entre comunidades) y de la diversidad beta (diferencias en la composición de taxones entre comunidades). La diversidad alfa fue estimada usando los números de Hill, mientras que la diversidad beta se estimó usando los índices de Sorensen y Morisita-Horn y comparada usando un análisis de similaridad (ANOSIM). Resultados: La composición de taxones fue distinta dentro (escala de mesohábitat) y entre los ríos (escala regional). Sin embargo, la composición de FFG cambió entre, pero no dentro de los ríos. La comparación de los números de Hill y de la composición de taxones sugiere que el tipo de mesohábitat es un factor determinante de la diversidad taxonómica pero no de la diversidad de FFG en distintas escalas. En la escala temporal, la diversidad alfa fue baja para los meses con altas precipitaciones (febrero-abril) y alta para la temporada seca (enero). La diversidad beta temporal fue alta para los periodos muestreados y los procesos de anidamiento fueron el componente más notable de la diversidad beta a lo largo del año. Conclusión: Los ríos de la Serranía de la Lindosa albergan una alta diversidad de macroinvertebrados acuáticos. La estacionalidad y las diferencias en la heterogeneidad del hábitat dentro de los ríos parecen determinar los patrones de diversidad observados a diferentes escalas temporales y espaciales. Profundizar en su estudio es necesario para entender el funcionamiento de estos ecosistemas y mejorar la habilidad para generar estrategias de manejo y conservación.
Phylogeny and latitudinal biogeography of the Papillomaviridae Family
Authors: Zambrano-Mila Marlon S. , Sibaja-Cordero Jeffrey A. , Alfaro-Núñez Alonzo 
Published: January 2021

Introduction: The latitudinal diversity gradient (LDG), a fundamental ecology pattern for higher organisms, has explained the increase of species diversity from the poles towards the tropics. Can we explain the biodiversity of Papillomaviruses based on this pattern? Objective: To analyse the phylogenetic diversity and phylogeography of most known genotypes and species belonging to Papillomaviridae Family isolated and molecularly characterized by distant latitudinal locations around the world. Methods: We collected 238 gene sequences encoding for the L1 and L2 viral capsid proteins from PaVE database. A geographical heat map based on the PV locations allowed to analyse the distribution and the number of PV species per country. Subsequently, a phylogenetic tree based on concatenated amino acid sequences L1 and L2 was constructed using a combination of Bayesian and Maximum Likelihood (ML) methods with Geneious version 11.2.1 and BEAST version 1.8.4. Statistical analysis with Principal Coordinate Analyses (PCA) using the Jaccard similarity index for presence-absence were carried out to test the similarity of the groups in the phylogenetic tree based on their viral species, the host and origin country lists. Distance-based redundancy analysis generated an ordination plot of the similarity of viral species list per group of the phylogenetic tree vs. host species per group, the country list per group and the number of samples per group. Finally, the significance of each model was tested by Analysis of variance (ANOVA). Results: A clear tendency of most of the papillomavirus (PV) species clustering in the Northern regions appeared. Our phylogenetic analyses largely support the taxonomic division into major papillomavirus genera (Alpha, Beta, Gamma, Delta, Lambda, Xi, Upsilon and Chi papillomaviruses), as reported in previous studies. Chelonid PV’s, a papillomavirus in turtles, appeared as the oldest group for all papillomaviruses. Minor inconsistencies were found within some of the major taxonomic groups explained by the exhausted phylogenetic analyses. Due to the different sampling data efforts with little replication in certain groups, hosts and countries, numerical models did not fit to the proposed hypothesis. Conclusions: Phylogeographical analysis allowed determining the global distribution of papillomaviruses species diversity per latitudinal location. As most of the papillomavirus (PV) species were clustered in the Northern regions, this may clearly represent a statistical bias because larger efforts in PV studies have historically been done in the US and Europe. The biogeographical trend of a latitudinal diversity gradient does not apply for papillomaviruses. Even though no support to the phylogeographical pattern of LDG for PV was obtained, our results may only be the product of a socio-economical-scientific artefact and not a natural phenomenon.
State of lipid metabolism in patients receiving androgen replacement therapy
Authors: Popov P. S. , Kournikova I. A. , Torshin V. I. , Malyutina N. N. 
Published: January 2021

Objective. To assess the state of lipid metabolism indicators and predict cardiovascular risk in patients with secondary (acquired) hypogonadism on the background of androgen replacement therapy. Testosterone replacement therapy is often used in modern medicine for diagnosed androgen deficiency in patients of any age group. There are quite a lot of publications devoted to diagnosis and treatment, but the effectiveness and safety of any therapy is determined by several factors, of which two were considered in the presented study dose-dependent effect and compliance.
Material and methods. Sixty two patients aged 30 to 52 years with androgen deficiency and a low risk of developing cardiovascular diseases were examined according to the Princeton Consensus criteria. Patients were divided into groups depending on the severity of androgen deficiency and the duration of therapy. Complaints, objective status, muscle strength, daily blood pressure and heart rate monitoring data, lipidogram and sex hormone indicators were evaluated.
Results. The obtained data suggest that androgen replacement therapy in doses that lead to an increase in the level of testosterone in the blood above the upper limit of reference values had a negative impact on the lipid spectrum and increased cardiovascular risk for this group of patients.
Conclusions. The analyzed approach to therapy of androgen deficiency should provide an individual adjustment of dosage on the background of determining the target blood testosterone level.
Preparation of primary glial tumor cell lines
Authors: Shamova T. V. , Sitkovskaya A. O. , Rostorguev E. E. , Kuznetsova N. S. , Kavitsky S. E. 
Published: January 2021

Objective. The aim of this work was to obtain the primary cell lines of brain malignant tumors using the explant method.
Materials and methods. Thirteen patients of both sexes, aged 22 to 66, were recruited. The tumor material of the patients was fragmented and placed in flasks with complete nutrient medium for glial tumor cells. Subsequently, the material was photographed at various stages of cultivation, the cell morphology was determined, and the rate of monolayer formation at the zero and first passages was assessed.
Results. As a result, thirteen primary human cell lines of glial tumors were obtained: six glioblastoma lines, two glioblastoma lines with anaplastic astrocytoma, one anaplastic oligodendroglioma line, one diffuse astrocytoma line, one oligoastrocytoma line and one diffuse protoplasmic astrocytoma line, one anaplastic astrocytoma line. In the culture of diffuse astrocytoma, there were observed the cells forming a network at the bottom of the flask. In the culture of anaplastic astrocytoma at a confluence of 3050 %, fibroblast-like cells were presented, and at a confluence of 100 %, a monolayer was formed with cells intimately adjacent to each other. In the culture of oligoastrocytoma, both fibroblast-like cells and islets of closely intertwined fusiform cells were observed. The same was typical for the cells of diffuse protoplasmic astrocytoma. Anaplastic oligodendroglioma during the first week of cultivation was represented mainly by round cells with a contrast agent, which subsequently attached and actively proliferated. At a confluence of 3080 %, fibroblast-like cells were observed, and at 100 %, spindle-shaped cells closely adjacent to each other. In cultures of glioblastomas, no specific character of cell growth was revealed: spindle-shaped, fibroblast-like cells and cells with long processes forming a network were encountered. Glioblastoma cultures against the background of anaplastic astrocytoma were represented by a network of cells with long processes.
At the zero passage, the rate of formation of a 100 % confluence monolayer ranged from 22 to 85 days. At the first passage, the cells reached a full monolayer within 4 to 25 days. At the zero passage, the longest time among all the samples to form the monolayer with a 100 % confluence needed glioblastoma lines on average 59 days. The shortest time to reach a 100 % confluence was required for cells of diffuse astrocytoma, anaplastic oligodendroglioma and glioblastoma against the background of anaplastic astrocytoma 2224 days.
Conclusions. In our work, it was shown that the explant method ensures the production of viable cells of glial tumors and the possibility of their further cultivation.
Epidemiologic features and treatment outcomes of pediatric pulmonary tuberculosis cases
Authors: ETİZ Pınar , ALTUNSU Ali Tanju 
Published: January 2021

Purpose: We here aimed to examine the epidemiologic features and treatment outcomes of pediatric pulmonary tuberculosis cases who were registered to the Adana Tuberculosis Dispensaries.
Materials and Methods: Fifty-six pediatric patients aged 0-18 years with the diagnosis of pulmonary tuberculosis were examined retrospectively in terms of demographic data, clinical characteristics and drug susceptibility test results, as well as treatment outcomes. Data were collected through medical record review.
Results: The mean age of the patients was 12.84 ± 5.62 years and 46.4% were female. Nine patients (16%) were aged 0-5 years, 4 (7.1%) were aged 5-10 years, 17 (30.3%) were aged 10-15 years and 26 patients (46.4%) were aged 15-18 years. Fourty-eight children was only pulmonary tuberculosis whereas eight possessed characteristics of both pulmonary and extrapulmonary tuberculosis. The most frequent symptoms were prolonged cough (80.3%) and weight loss (73.2%). Drug susceptibility test was performed only 22 patients. Multidrug resistance was determined in one patient. Among the 56 children for whom treatment outcome was documented, 39 (69.6%) were completed treatment, 16 (28.5%) were cured and 1 (1.7%) had treatment failure.
Conclusion: It was observed that the signs and symptoms of our patients were compatible with the literature. The epidemiological characterization of pediatric patients with pulmonary tuberculosis helps to provide a better diagnostic approach in this population.
Kardiyovasküler hastalığı olan bireylerde tamamlayıcı ve alternatif tıp yöntemlerinin kullanımı
Authors: ARSLAN Sevban , GÖKÇE Esma , DENİZ DOĞAN Sevgi , ÖZGEN Refiye , ŞİŞMAN Hamide , ALPTEKİN Dudu , GEZER Derya 
Published: January 2021

Amaç: Bu araştırma kardiyovasküler hastalığı (KVH) olan bireylerin tamamlayıcı alternatif tedavi (TAT) yöntemlerini kullanma durumlarının belirlenmesi amacıyla planlanmıştır.
Gereç ve Yöntem: Araştırma tanımlayıcı ve kesitsel olarak, Aralık 2017- Şubat 2018 tarihleri arasında, bir üniversite hastanesinin kardiyoloji kliniğinde yatmakta olan 110 hastayla yapılmıştır. Veriler literatür taranarak elde edilen ‘Kişisel Bilgi Formu’ ve ‘Bireylerin TAT yöntemleri kullanım durumu’ ile ilgili soruları içeren form aracılığıyla toplanmıştır.
Bulgular: Araştırmada hastaların %80’i TAT kullanmakta, en fazla bitkisel tedavi, sonrasında dua ve egzersiz yer almaktadır. Yapılan regresyon modellemesinde TAT kullanımında yaş, eğitim düzeyi, aile yapısı ve ilaç hakkında bilgi sahibi olma durumunun bağımsız etkileyici faktörler olduğu belirlenmiştir. Hastaların % 44,5’i TAT kullanımında etkili ilk faktörün arkadaş ve akraba olduğunu bildirmişlerdir. TAT kullanım nedenlerinden ilk üç sırada; KVH tedavisi için %19, sağlığa faydalı olduğunu düşündükleri için %17, iyileşmek/hastalıktan kurtulmak için %14 olarak yer almıştır. Hastalar %52 oranında TAT yönteminin olumlu olarak etkilendiklerini bildirmişlerdir.
Sonuç: Hemşireler hastaların TAT yöntemlerinden hangisini kullandığı belirlemeli ve bilgilendirerek doğru yönlendirmelidir.
IDENTIFY FAQ KNOWLEDGE GAP IN IT HELPDESK USING KNOWLEDGE MAPPING CASE STUDY: HALOSIS BPS-STATISTICS
Authors: Suwiyanto Viktor , Sensuse Dana Indra 
Published: January 2021

One important feature of IT HelpDesk, such as HaloSIS, is knowledge base. This feature disseminate knowledge about problem solving knowledge. This knowledge base goals were to assist service desk for giving fast solution to problem reported by user call and to facilitate learning about problem solving for new member of technical support team. To improve problem solving performance, technical support team can update or add this knowledge base with new problem solving knowledge. However, until this day this knowledge base was not updated. This can lead HaloSIS into tacit knowledge problem. This tacit knowledge remain in technical support head and not documented to knowledge base. To identify new knowledge that was not yet transferred to knowledge base, HaloSIS team need to identify knowledge gap that happen in HaloSIS knowledge base. There is a tool of knowledge management that can be used for identifying knowledge gap. This tool is knowledge mapping. Knowledge map can be used to representing the incompleteness of the current knowledge network that will enable to attain a sustainable future. This study propose knowledge map building from troubleshooting log in HaloSIS. This study build current knowledge map based on FAQ knowledge and merge it with knowledge found in troubleshooting log. The methodology to build knowledge map was the 6-step method from Kim's research. The data analysis method used is qualitative data analysis method using thematic analysis. From this knowledge map this study found 15 new know-how knowledge and recomendation to update 7 know-how knowledge. However, there are some limitations of this study which only maps the online presence system. For future research, it is necessary to build a knowledge map for all services in HaloSIS comprehensively to maintain, renewal, and seeking for knowledge.
Pattern of flame burn injury in Dibrugarh district of Assam: An autopsy based analysis
Authors: Debbarma Swapan 
Published: January 2021

Burn injuries are the second major cause of trauma-related deaths only exceeded by motor vehicular accidents, both in the developing and developed world. Burn due to flame or fire is common in this region.To find out epidemiological patterns and cause of death in the flame burn as data on it is not available even though the most common cause of burn in this region.This cross-sectional observational study was carried out at the Department of Forensic Medicine, Assam Medical College, Dibrugarh from 1st July 2014 to 30th June 2015.A total of 224 cases died of flame burn brought for autopsy were taken for study. A thorough post-mortem examination was done on every case following standard procedure. Data were recorded in the proforma especially prepared for this purpose.Out of 1436 medico-legal deaths, 224 (15.60%) was due to flame burn. The majority of victims were female 186 (83.03%), married, and belong to 21-30 years of age group. Victims were mostly from rural areas, unemployed, and literates. Kitchen 129 (57.60%) was the main place of occurrence. The majority suffered more than 80% burns of TBSA and died within 24 hours 92 (41.07%). The hypovolemic shock was the most common cause of death.Until the economic condition is improved and lifestyle changes among the people, the fire-related problem must rise in countries like India.Young age groups and unemployed were most among victims. Burn incidents decrease with increasing education level. Economic improvement and change of lifestyles will help in reducing burn-related problems.
Pet owners' online information searches and the perceived effects on interactions and relationships with their veterinarians
Authors: Lai Nanette , Khosa Deep K. , Jones-Bitton Andria , Dewey Cate E. 
Published: January 2021

Objective: To explore pet owners’ online search experiences for pet health information and the perceived effects on their interactions and relationships with veterinarians.
Background: Few studies have examined pet owners’ online searches for pet health information; even less is known about how these search experiences may impact pet owners’ interactions and relationships with veterinarians, including any effects on bond-centered care.
Methods: Qualitative study consisting of five focus groups conducted with 26 pet owners in the Greater Toronto Area, Ontario, Canada, between June to September 2016. All interviews were audio-recorded and transcribed verbatim. QSR NVivo 11® was used to facilitate organisation of focus group data for thematic analysis.
Results: Participating pet owners frequently referred to their relationships with veterinarians when discussing experiences searching online for pet health information. Owners reported choosing either to disclose or withhold declaring their online searches to veterinarians, depending on whether participants perceived a beneficial or detrimental impact on a “good” professional relationship with their veterinarian. Perceptions of veterinarians' reactions towards declaration of online searches were mixed, and influenced pet owners’ views of the existing relationship.
Conclusion: Pet owners viewed their veterinarians as their most trusted source of pet health information, but many owners also wanted supplemental information from online searches. Owners preferred veterinarians refer them to online pet health resources, ideally those affiliated with the veterinary profession. Searching for pet health information online does not displace veterinarians’ guidance. Rather, the veterinarian-owner relationship was perceived to be strengthened when online searches were openly discussed with veterinarians.
Implications: Findings offer insight into pet owners’ expectations of veterinarians within the context of online pet health information, providing ideas for veterinarians to strengthen bonds with owners such as; showing support of owners’ online pet health information searching by recommending resources and considerations about communicating professional opinions to owners regarding online information.
An Examination of CACREP Curriculum Standards From a Psychiatric Rehabilitation Recovery Model Perspective
Authors: Peterson Sonia , Olney Marjorie 
Published: January 2021

PurposeThis article compares disability-related social justice, inclusion, and psychiatric rehabilitation recovery model principles with the Council for Accreditation of Counseling and Related Educational Programs (CACREP, 2016) counseling curriculum standards in order to clearly articulate areas in the current standards that need revisions.MethodWe conducted a structured analysis of the CACREP curriculum standards in light of the Psychiatric Rehabilitation Association's 12 principles and values.ResultsSections of the CACREP curriculum standards are very beneficial for RC education, such as the additional emphasis on Diagnostic and Statistical Manual 5 (DSM-5) diagnoses, human development theories, crisis intervention, trauma-informed strategies, and the use of evidence-based counseling practices. Standards emphasizing the importance of client autonomy and respect, quality of life, community inclusion, advocacy, access, and evidence-based recovery model approaches need to be strengthened. The term “disability” does not currently appear in the eight core curriculum standards.ConclusionsThe authors offer specific course materials and activities that rehabilitation counselor (RC) educators can begin to incorporate into the design of their curriculum in order to better infuse ethical, social justice, disability inclusion, and recovery model principles into required coursework. Recommendations for changes to the CACREP curriculum standards are discussed.
Positively Engaged Pathways: A Brief Pre-Employment Intervention for Transition Age Youth With Disabilities
Authors: Fleming Allison R. , Phillips Brian N. , Gilfillan Elizabeth H. , Gray Joy , McCloskey Megan 
Published: January 2021

PurposeThis study was a pilot of a brief psychological capital (PsyCap) intervention with a sample of transition age youth with disabilities (N = 24) in a school setting. Substantial evidence supports the relationship between PsyCap and employment outcomes. Transition age youth with disabilities are an important target for pre-employment counseling interventions due to a lack of parity in postsecondary outcomes. Researchers have successfully boosted PsyCap in adult samples using brief interventions.MethodsPsyCap and vocational outcome expectancy (VOE) were compared in a repeated measures design.ResultsPsyCap and VOE, and PsyCap and school performance were significantly correlated in our sample, but no differences were observed in PsyCap or VOE postintervention.ConclusionAs the first known effort to develop a PsyCap driven pre-employment intervention for transition age youth with disabilities, our experience and findings provide several implications for practice and future research. Aspects of the intervention to be modified for future efforts are presented.
Desarrollo de habilidades de análisis crítico a partir de la identificación de errores en diagramas asociados a los efectos de las ondas sísmicas
Authors: Sellés-Martínez José 
Published: January 2021

Se analiza un conjunto de modelos tridimensionales que representan la forma en que las ondas sísmicas afectan los materiales que atraviesan, generando vibraciones y desplazamientos y contienen diversos errores. Entre ellos se destaca la dirección en que las ondas P y S llegan a la superficie, la exageración de los desplazamientos asociados a las ondas P y S, haciéndoselos comparables con los producidos por las ondas Love y Raileigh y asignándoseles propiedades destructivas equivalentes a éstas. Tampoco se tienen en cuenta las propiedades mecánicas reales de los materiales que constituyen las cercas y tendidos eléctricos frente a la deformación inducida por las ondas ni los esfuerzos generados. Se propone utilizar estos materiales, junto con ilustraciones correctas, para que los estudiantes desarrollen habilidades tanto para la transformación de conceptos teóricos en diagramas y materiales gráficos como para el análisis crítico del material de estudio.