bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishing reference curves for vital tissue perfusion using quantitative near-infrared fluorescence imaging with indocyanine green
Authors: Tange Floris P. , Peul Roderick C. , Hoven Pim van den , Koning Stefan , Kruiswijk Mo W. , Faber Robin A. , Verduijn Pieter S. , Rijswijk Carla S.P. van , Galema Hidde A. , Hilling Denise E. , Dijk Sam P.J. van , Ginhoven Tessa M. van , Keereweer Stijn , Mureau Marc A.M. , Feitsma Eline A. , Noltes Milou E. , Kruijff Schelto , Driessen Caroline , Achiam Michael P. , Schepers Abbey , Schaik Jan van , Mieog J. Sven D. , Vahrmeijer Alexander L. , Hamming Jaap F. , Vorst Joost R. van der 
Published: December 2024

Abstract

Purpose
Assessment of tissue perfusion using near-infrared fluorescence (NIR) with indocyanine green (ICG) is gaining popularity, however reliable and objective interpretation remains a challenge. Therefore, this study aimed to establish reference curves for vital tissue perfusion of multiple target tissues using this imaging modality.
Methods
Data from five prospective study cohorts conducted in three Dutch academic medical centres between December 2018 and June 2023 was included. Quantitative analysis of ICG NIR fluorescence video’s using time-intensity curves was performed in ten target tissues, including the colon, ileum, gastric conduit, deep inferior epigastric artery perforator (DIEP) flap, skin of the foot, trachea, sternocleidomastoid muscle (SCM), carotid artery, parathyroid gland, and skin of the neck.
Results
A total of 178 patients were included in this study, representing 303 target tissues. Three different patterns of reference curves were identified. Seven out of ten tissues showed a reference curve with rapid inflow (median time-to-max (tmax): 13.0-17.8 sec, median maximum normalized slope (slope norm): 10.6–12.6%/sec), short outflow (median area-under-the-curve of tmax + 60sec (AUC60): 65.0-85.1%) followed by a gradual/absent outflow. Secondly, the DIEP flap and SCM showed a reference curve with slightly longer inflow (median tmax: 24.0, 22.0 sec, median slope norm: 9.3, 9.7%/sec respectively) and reduced outflow (median AUC60: 89.1, 89.0% respectively). Thirdly, the skin of the foot showed slow inflow (median tmax 141.1sec, median norm slope 2.1%/sec) without outflow.
Conclusion
This study demonstrates reference curves for vital tissue perfusion of multiple target tissues identified with ICG NIR fluorescence imaging, providing a critical step towards the clinical implementation of this technique.
Prediction on the Demand for Preschool Resources in Shaanxi under the Three-Child Policy
Authors: Li Dong , Yu Yanyan , Sui Mengyao 
Published: December 2024

Abstract

The implementation of the three-child policy in China will impact the shifting trends in the newborn population, thereby influencing the size and resource needs of preschool education. In order to accomplish the objective of high-quality and sustainable preschool development, it is crucial to promptly predict the demand for preschool resources based on demographic changes. However, current research on demand forecasting of preschool resources fails to consider the impact of demographic and socioeconomic changes on the preschool system within the context of the three-child policy, potentially resulting in unsatisfactory research outcomes. This paper utilizes the system dynamics method to establish a preschool education resource prediction model based on an analysis of the interplay between population, economy, and various factors within the preschool education system. The model parameters are optimized through the application of the multi-objective lioness optimization algorithm. Subsequently, the study predicts the shifts in demand for preschool education resources (such as preschool education place demand, teacher demand, and capital demand) in Shaanxi from 2022 to 2060. The findings indicate an 'M'-shaped trajectory in both preschool enrollment and teacher demand. Specifically, the number of schools and teachers is projected to increase to 10,491 schools and 188,800 teachers, respectively, in 2022-2023. Subsequently, between 2024 and 2026, there will be a reduction to 8,535 schools and 153,600 teachers. In 2027, a period of rapid growth is anticipated, with an increase to 9,663 schools and 173,900 teachers by 2029. However, between 2030 and 2060, these figures are expected to decline to 6,643 schools and 119,600 teachers. Additionally, the overall trend in the evolving demand for funding in preschool education follows an 'N'-shaped pattern. Specifically, the funding is projected to continue increasing to 23.63 billion Yuan between 2022 and 2024, followed by a reduction to 21.92 billion Yuan from 2025 to 2026. Subsequently, there will be a period of rapid growth from 2027 to 2029, with funding reaching 28.68 billion Yuan. Afterward, the funding will experience a gradual increase, eventually reaching 81.75 billion Yuan from 2030 to 2060. In general, the demand for preschool education resources in Shaanxi will undergo a brief phase of rapid growth in the coming years due to the impact of the three-child policy. However, it will eventually transition into a period of decline. Consequently, the government and relevant education authorities should carefully assess the disparities between short-term and long-term requirements and develop a scientific and prudent plan for preschool resources.
Genome-scale activation screen reveals lncRNA HNF1A-AS1 as a novel therapeutic target for pancreatic cancer metastasis
Authors: Lei Shan , Zhang Zhixue , Zeng Zhirui , Cao Wenpeng , Sun Yating , Li Dahuan , Pan Jigang , Wu Yingmin , Zhang Tuo , Chen Tengxiang 
Published: December 2024

Abstract

Background
Long non-coding RNAs (LncRNAs) have emerged as pivotal biomarkers and regulators across various cancers. In pancreatic cancer (PC), however, the mechanisms underlying the expression and functional roles of lncRNAs remain inadequately elucidated.
Methods
CRISPR/CRISPR-associated protein 9 (Cas9) single-guide RNA (sgRNA)-pooled lncRNA libraries were used to screen for the critical lncRNAs regulating PC metastasis. The expression levels of lncRNA HNF1A-AS1 were quantified in PC cell lines and clinical samples using qRT-PCR. Investigations into HNF1A-AS1's impact on PC cell migration and invasion were conducted through both loss-of-function and gain-of-function approaches. A range of techniques, including fluorescence in situ hybridization (FISH), mRNA sequencing, RNA immunoprecipitation (RIP), bioinformatics analysis, dual-luciferase reporter assays, RNA pull-down assays, ChIP-PCR, and rescue experiments, were employed to unravel the competitive endogenous RNA (ceRNA) network regulated by HNF1A-AS1.
Results
The research identified HNF1A-AS1 as a novel and influential lncRNA that acts as a pro-metastatic factor in PC. Compared to normal controls, HNF1A-AS1 levels were significantly elevated in PC cell lines and tissue samples. Elevated HNF1A-AS1 expression correlated with increased lymph node metastasis and poorer overall survival in patients with PC. Knocking down HNF1A-AS1 substantially reduced metastasis, whereas its overexpression exacerbated it. Mechanistically, HNF1A-AS1 promotes an oncogenic splice switch from the standard isoform CD44s to the variant isoform CD44v (3–10), acting as a scaffold for the binding of CD44 pre-mRNA to U2SURP. The levels of HNF1A-AS1 and CD44v (3–10) serve as indicators of poor prognosis. Furthermore, SNAI2 was shown to specifically bind to the HNF1A-AS1 promoter, thereby activating its transcription. Antisense oligonucleotides (ASOs) targeting HNF1A-AS1 also significantly inhibited cancer metastasis.
Conclusions
SNAI2’s role in enhancing HNF1A-AS1 transcription underscores the critical function of HNF1A-AS1 in promoting PC metastasis through modulation of CD44 alternative splicing via U2SURP. Targeted silencing of HNF1A-AS1 presents a promising therapeutic avenue for patients with PC.
Incidence and risk factors of low birth weight in a rural district of Bangladesh: a prospective cohort study
Authors: Bhattacharjee Pallab , Islam Md Shafiqul , Roy Arunangshu , Amin Zannatul Ferdush , Rahman Nayema Bintye , Shahed M A , Akter Fahmeda , Chowdhury Nabidul Haque , Mitra Dipak Kumar , Khanam Rasheda , Baqui Abdullah H. , Ahmed Salahuddin 
Published: December 2024

Abstract

Background
Low birth weight (LBW, birthweight < 2500 gm) is associated with a significantly increased risk of morbidity and mortality during infancy and is an important contributor to childhood developmental delays.
Methods
To estimate the incidence of LBW and identify its risk factors, we analyzed data from a population-based cohort study, the Aetiology of Neonatal Infections in South Asia, conducted in two sub-districts of Sylhet district of Bangladesh. The study followed 24,271 pregnant women from 2011 to 2013. The final analysis included 17,643 singleton liveborn babies with birthweight data available. To identify associated factors of LBW, we calculated adjusted risk ratios (ARRs) and 95% confidence intervals (CIs) using multivariable modified Poisson regression with robust variance.
Results
The incidence of LBW was 27.61% (95% CI: 26.96%-28.28%). Significant risk factors of LBW were no education of father [ARR 1.09 (95% CI: 1.01–1.17)]; non-Muslim religion [ARR 1.32 (95% CI: 1.20–1.45)]; lowest household quintile [ARR 1.31 (95% CI: 1.19–1.44)], second lowest household quintile [ARR 1.26 (95% CI: 1.15–1.38)], and middle household quintile [ARR 1.16 (95% CI: 1.06–1.26)], compared to the highest quintile; primiparity [ARR 1.59 (1.49–1.69)]; maternal mid-arm circumference (MUAC) < 22 cm [ARR 1.19 (95% CI: 1.13–1.25)]; tobacco consumption during pregnancy [ARR 1.15 (95% CI: 1.08–1.23)]; pregnancy complications [ARR 1.10 (95% CI: 1.03–1.17)]; no antenatal visits with qualified providers [ARR 1.16 (95% CI: 1.07–1.25)]; no antenatal iron consumption [ARR 1.12 (95% CI: 1.04–1.21)]; <2 doses of antenatal TT immunization by mothers [ARR 1.19 (95% CI: 1.09–1.31)] and no TT doses [ARR 1.16 (95% CI: 1.09–1.23)] ; residence ≥ 15 km from sub-district hospitals [ARR 1.12 (95%CI 1.07–1.18)]; gestational age at birth: <32 weeks [ARR 1.88 (95% CI: 1.71–2.06)], 32–33 weeks [ARR 1.61 (95% CI: 1.46–1.77)], and 34–36 weeks[ARR 1.42 (95% CI: 1.34–1.51); and female baby [ARR 1.19 (95% CI: 1.14–1.25)].
Conclusions
The burden of LBW was high in rural Bangladesh. Multiple antenatal factors, including maternal undernutrition, tobacco use, and suboptimal antenatal care were associated with increased risk of LBW. Maternal and child health programs should prioritize interventions to address these factors.
Clinical trial number:
Not applicable
WITHDRAWN: Object Detection: A comprehensive study of famous Deep Learning approaches with case study of Helmet Detection dataset
Authors: Tajane Kapil , Khairmode Atharva , Kore Atharva , Khutale Prathmesh , Girawale Sachin , Pitale Rahul 
Published: December 2024

Abstract

The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference.
Colorectal cancer survivorship program at a single tertiary centre :– Has service provision changed after Covid-19?
Authors: Menadue Rachael , Tiffany Lea , Satheakeerthy Shriranshini , Sakalkale Aditya , Wei Matthew , Reid Fiona , Faragher Ian , Yeung Justin M 
Published: December 2024

Abstract

Purpose
Surveillance after colorectal cancer (CRC) resection is an important aspect of survivorship care. This study aimed to assess whether there were any changes to post-operative surveillance in non-metastatic CRC patients, pre and post-COVID pandemic in Victoria.
Methods
All CRC patients (stage I-III) who underwent curative surgery at Western Health, Victoria, Australia were included. Surveillance included a three-monthly clinical review and carcinoembryonic antigen (CEA) up to 18 months, and CT imaging and colonoscopy at 12 months following surgical resection.
Results
Between 2019-2022, 380 patients were identified. Stage III patients had the highest uptake with regards to clinical reviews, CEA testing and 12-month CT (83.3%, 60.3% and 85.5% respectively) while Stage I patients had the lowest (52.7%, 35.7% and 75.5% respectively) (p<0.05). Colonoscopy surveillance was low regardless of stage (66.3%, 59.8% and 59.7% of Stage I, II and III respectively). Uptake of CEA, clinic reviews and colonoscopy did not vary during our study period. More patients underwent 12-month CT following the COVID pandemic (87.0%) compared to pre-COVID (73.1%) or during COVID (76.0%, p<0.05). There was no difference in 18-month mortality and overall recurrence during our study timelines.
Conclusion
Patients with earlier stage CRC had lower rates of adherence to surveillance protocols, particularly with regard to CEA monitoring and colonoscopic surveillance. Adherence to surveillance and surveillance patterns were not meaningfully altered by the intra or post COVID-19 era.
Near-unity spontaneous emission factor InP surface-emitting lasers based on quasi-bound states in the continuum
Authors: Wong Wei Wen , Huang Xiaoying , Lem Olivier Lee Cheong , Jagadish Chennupati , Tan Hark Hoe 
Published: December 2024

Abstract

With their compact form factor and excellent beam quality, surface-emitting lasers based on optical bound states in the continuum (BICs) have emerged as a promising alternative to vertical cavity surface-emitting lasers. However, structural damage inevitably caused by top-down fabrication processes remains as a major obstacle that limits device performance. Here, we overcome this bottleneck by demonstrating surface-emitting quasi-BIC lasers fabricated with a bottom-up, etching-free process. We epitaxially grow arrays of InP nanosheets with atomically smooth sidewalls as the active medium, then engineer a quasi-BIC cavity within the arrays by perturbing in-plane inversion symmetry, strategically combining the active layer with the laser cavity for optimal light-cavity coupling. Furthermore, by growing wurtzite-phase nanosheets, we leverage the dipole selection rule associated with the hexagonal crystal structure for side mode suppression, achieving stable single-mode lasing at room-temperature. With lasing threshold as low as 14 μJ cm-2 pulse-1, our devices exhibit directional and linearly polarised far-field emission. Remarkably, through precise spectral tuning of the quasi-BIC resonance, we record two orders of magnitude increase in the spontaneous emission factor to as high as 0.8. This work demonstrates the potential of bottom-up BIC lasers in surpassing the performance of their top-down fabricated counterparts and reaching thresholdless lasing.
Genetic determinants of the phenotype in a Swedish cohort of patients with hypertrophic cardiomyopathy
Authors: Kissopoulou Antheia , Fernlund Eva , Karlsson Jan-Erik , Green Henrik , Gunnarsson Cecilia 
Published: December 2024

Abstract

Background and Aims
Hypertrophic cardiomyopathy (HCM), the most common inherited cardiomyopathy, is characterized by phenotypic and genetic heterogeneity. The present study describes the genotype data of a Swedish cohort of patients with HCM, the largest genetics study on Swedish HCM patients to date. The primary aims of this study were to unravel the main genetic findings and explore genotype-phenotype associations in this HCM cohort.
Methods and Results:
Longitudinal data on 225 unrelated HCM index patients from the Southeast health care region in Sweden from 2010 until 2021 were assessed retrospectively. Patients were 46 ± 15.5 years-old, 67.6% males. In the cohort, 172/225 (76.4%) had genetic testing, of whom, 65/172 (38%) were considered genotype positive (G+) for a pathogenic/ likely pathogenic variant, mainly in the two most common sarcomeric genes: MYBPC3 (57%) and MYH7 (34%). In 43% (74/172) of patients no pathogenic variant was detected. Genotype positive patients (G+) were characterized by younger age (p = 0.010), higher prevalence of family history of HCM (p < 0.001), greater maximum left ventricle wall thickness (p=0.03) and an increased incidence of sudden cardiac death (SCD) (p=0.045). At first clinical screening, HCM was diagnosed in 28/65(43%) in the G+ families and in 2/74 (2.7%) G-families (p<0.001).
Conclusion:
Genotype-positive HCM patients differ with respect to age at presentation, family history of the disease, morphology, incidence of SCD and presence of HCM in their family members at first clinical assessment from genotype-negative patients. Genotype negative status in this HCM cohort, though, did not confer immunity from adverse complications.
Identification of diagnostic biomarkers and potential therapeutic drugs in focal segmental glomerulosclerosis with metabolic syndrome by integrating bioinformatics and machine learning
Authors: Yao Tianwen , Wang Qingliang , Han Shisheng , Xu Yanqiu , Chen Min , Wang Yi 
Published: December 2024

Abstract

Purpose Immune system dysregulation plays a pivotal role in focal segmental glomerulosclerosis (FSGS) and metabolic syndrome (MS). This study aimed to identify core diagnostic genes and potential therapeutic drugs for FSGS patients with MS. Methods We obtained two FSGS and one MS datasets from the GEO database. DEGs and module gene were identified via Limma and WGCNA. Then, functional enrichment analysis, PPI network construction, and machine learning algorithms were applied to identify and analyze immune-associated genes. Afterwards, the nomogram and ROC curve were used to evaluate the diagnostic value and screen core genes. Finally, immune cell dysregulation was investigated in FSGS, and connectivity map (cMAP) analysis was conducted to identify potential therapeutic small molecule compounds. Results MS dataset yielded 756 DEGs, and the integrated FSGS datasets yielded 5257 module genes. 133 genes were identified from the intersection of MS and FSGS. Following the construction of PPI network, 42 node genes were filtered. Then, eight hub genes were obtained through machine learning screening, which were further evaluated by nomogram and diagnostic value. Among them, six core genes had high diagnostic values. FSGS patients had a higher level of resting natural killer cells, monocytes, and activated dendritic cells and meanwhile lower levels of plasma cells, follicular helper T cells, resting dendritic cells, and resting mast cells. Finally, through cMAP analysis, we identified ten small molecule compounds that might work as the potential therapeutic drugs for FSGS patients with MS. Conclusion Six immune-related core genes were identified (STAT3, CX3CR1, CCDC148, TRPC6, CLMP, and CDC42EP1), and ten small molecule compounds were obtained. This study could provide core diagnostic genes and potential therapeutic compounds for FSGS patients with MS.
Innovative Approach To Distraction Osteogenesis Using A Novel External Fixator Design. In Vivo Experimental Rabbit Model
Authors: slimi fathia , bahri Awatef hadj , Trigui Moez , guidra racem , ellouz zoubeir , rebai Tarek , elfeki Hafed , elfeki abdelfatteh , Hassib Keskes , samira jebahi 
Published: December 2024

Abstract

Distraction osteogenesis, bone lengthening, is an established surgical technique for bone lengthening. The procedure involves making osteotomies, in the bone, and using a device to gradually separate, or “distract”, the bone fragments. New Zealand white male rabbits underwent distraction osteogenesis on the right tibia and lengthening by means of external fixators at two different time points ; a speed of 1.4 mm/day in 15 days and 2.1 mm/day in 10 days. The regenerated callus was carrefully removed and were subjected to biological and physicochemical analyses such as Scanning Electron Microscope (SEM), Fourier Transform Infrared spectroscopy (FTIR) and Nuclear Magnetic Resonance (RMN) analyses. For the period of five weeks, FTIR results showed that the mineral phases of the regenerated tissue of two groups were made up of biological apatite crystals, which are defined as a phosphocalcium carbonated. This IR spectra confirmed the presence of phosphate (PO4-3), hydroxyl (OH)- and carbonate (CO3 -2) groups. NMR demonstrated that PO43- group of the regenerated bone has practically a homogeneous and unique environment: chain PO4 - PO4 which represents the skeleton of the bone mineralization. SEM showed that at the same period of elongation: endost appearance evolved to more homogeneous appearance similar to healthy bone. However, the fast-lengthening group presented less homogeneous appearance, and a less hard cortical tissue formation also, the haversian aspect was incomplete. Histological analyses demonstrated that ossification was taken place according to three modalities: endochondral, endoconjunctive and transchondroid. As conclusion, the bone rearrangement during rapid lengthening is delayed while for the slow lengthening group the regenerated bone is strongly ossified. The lamellae are organised into concentric circles, which surround a vertical Haversian canal.
A highly photostable monomeric red fluorescent protein
Authors: Xu Pingyong , Ding Ya , He Wenting , Wang Kunhao , Xue Fudong , Zhao Shiqun , Pan Leiting , Chen Liangyi , Yuan Lin 
Published: December 2024

Abstract

A highly photostable and bright green fluorescent protein (FP), along with its monomeric variants, has recently been reported. These FPs enable long-term live-cell imaging. However, red FPs with similarly desirable properties remain scarce. Here, we introduce mScarlet3-S2, which is up to 29 times more photostable than its precursor, mScarlet3, and surpasses all current red FPs in photostability. Using mScarlet3-S2, we imaged mitochondrial cristae dynamics with high spatiotemporal resolution over several minutes using structured illumination microscopy (SIM), with significantly less photobleaching than mScarlet3 variants. mScarlet3-S2 holds great potential for super-resolution and long-term live-cell imaging applications.