bluuebunny/crossref_metadata_2025_split huggingface dataset:
VPS28 regulates triglyceride synthesis via ubiquitination in bovine mammary epithelial cells
Authors: Liu Lily , Wang Jinhai , Zheng Xianrui , Zhang Qin 
Published: December 2024

AbstractVPS28 (vacuolar protein sorting 28) is a subunit of the endosomal sorting complexes required for transport (ESCRTs) and is involved in ubiquitination. Ubiquitination is a critical system for protein degradation in eukaryotes. Considering the recent findings on the role of ubiquitination in the regulation of lipid metabolism, we hypothesized that VPS28 might affect the expression of genes involved in milk fat synthesis. To test this hypothesis, we modulated VPS28 expression in the bovine mammary epithelial cell line (MAC-T) and measured the effects on triglyceride (TG) synthesis using lentivirus-mediated techniques. The results showed that VPS28 knockdown significantly upregulated the levels of the fatty acid transporter CD36 molecule (CD36) and adipose differentiation-related protein (ADFP), leading to increased TG and fatty acid production, along with elevated ubiquitin (UB) levels, while reducing proteasome activity. In contrast, VPS28 overexpression increased CD36 levels while not significantly affecting ADFP or TG levels, with a trend toward reduced lipid droplets and increased UB expression and proteasome activity. In addition, inhibition of the ubiquitin-proteasome system and the endosomal-lysosomal pathway using epoxomicin and chloroquine, respectively, further increased CD36, ADFP, and TG levels, thereby enhancing cell viability. These in vitro findings were validated in vivo in a mouse model, where VPS28 knockdown increased mammary CD36, ADFP, UB expression, TG content, and lipid droplets without pathological changes in mammary tissue or blood TG alterations. These results confirm the pivotal role of VPS28 in regulating TG synthesis via the ubiquitination pathway, offering novel insights into the molecular mechanisms of milk fat production in a bovine cell model.
Hemodynamic comparisons of different shunt positions and geometrical model simplification strategies in the simulation of transjugular intrahepatic portosystemic shunt (TIPS)
Authors: Yang Liu , Zhang Yitao , Wang Tianqi 
Published: December 2024

AbstractTransjugular intrahepatic portosystemic shunt (TIPS) is a widely used surgery for portal hypertensive patients, whose potential postoperative complications are closely related to the hemodynamic condition of the portal venous system. The selection of shunt position in the surgery may affect the postoperative hemodynamics; however, it is difficult for clinical studies to investigate the influence. Therefore, this study aims to employ the computational model simulating TIPS to compare the hemodynamic differences resulting from different shunt positions, and also to investigate the influences of different geometrical model simplification strategies used in the TIPS simulation. For this purpose, the clinical data of two representative patients were retrospectively collected, based on which, the computational hemodynamic models of the portal venous systems after TIPS were constructed, incorporating three typical shunt positions (i.e. shunt at the left/main/right portal vein) and three types of geometrical model simplification. Results showed that among the models with different shunt positions, the area-averaged flow velocity magnitudes in the shunts were very similar, while the model with shunt at the main portal vein showed the lowest postoperative portal pressure and the smallest area of high wall shear stress near the portal venous bifurcation. Among the models using different geometrical model simplification strategies, the simulated blood pressures at the main portal veins were very similar, but showed marked differences near the shunt inlets. Moreover, the area-averaged flow velocity magnitudes in the shunts were almost the same, while the velocity distributions differed a lot, leading to the different spatial distributions of wall shear stress near the portal venous bifurcations and shunt walls. These results on one hand suggested that placing the shunt at the main portal vein is more beneficial for the patient; on the other hand, they proved the feasibility of utilizing simplified model to save computational cost without losing the accuracy when the pressure at the main portal vein is mainly focused on. These findings would assist clinical decision-making and promote more accurate and efficient TIPS simulations.
Thrust-dominated unilateral rupture of a blind listric fault associated with the 2024 Hualien earthquake
Authors: Zheng Ao , Yu Xiangwei , Bai Chengyuan , Xu Wenbin , Qian Jiaqi , Zhang Wenbo , Chen Xiaofei 
Published: December 2024

AbstractThe 2024 Hualien Mw 7.4 earthquake struck the Longitudinal Valley, which accommodates the partial collision between the Eurasian and Philippine Sea plates. As the most significant event in Taiwan since the 1999 Chi-Chi Mw 7.6 earthquake, it presents a distinct opportunity for investigating the current rupture behavior related to the northern Longitudinal Valley. The spatiotemporal rupture process of the Hualien earthquake is reconstructed through the analysis of geodetic and seismic observations. We demonstrate that the Hualien earthquake occurs on a blind listric fault, manifesting as a unilateral rupture primarily extending toward the NNE. The slip distribution exhibits a compact pattern dominated by the thrust faulting. As indicated by the increased Coulomb failure stress, the 2022 Chihshang Mw 6.5 and Mw 6.9 earthquakes cause a triggering effect on the Hualien earthquake. The Hualien earthquake also promotes the occurrence of a seismic swarm at the southernmost tip of its rupture area.
The effect of push frequency and stroke time on wheelchair maneuverability among wheelchair tennis athletes on hard tennis court
Authors: Esak Ajitkumar , Hamzaid Nur Azah , See Edelene , Khoo Selina 
Published: December 2024

AbstractWheelchair propulsion is a fundamental skill in wheelchair sports, particularly in wheelchair tennis. To achieve optimal mobility during wheelchair athletic performance, it is essential to consider propulsion techniques. This study examines the effect of push frequency and stroke duration on wheelchair maneuverability, measured by velocity during propulsion, among wheelchair tennis athletes. The athletes (N = 9; 5 elite and 4 novice) performed three tests; namely the comfortable speed propulsion test, sprint test and round-trip test; with and without holding their racquet, over their hard court. Results revealed that push frequency had positive correlation with propulsion velocity (r = 0.840, p < 0.001) while stroke time was negatively correlated with velocity (r = -0.859, p < 0.001). Propulsion performance between elite and novice wheelchair athletes, and between propelling with and without racquet were also reflected through these parameters. The rate of perceived exertion (RPE) had significantly positive but low correlation with velocity and push frequency, and significant negative correlation with stroke time. This study could serve as a recommendation for wheelchair tennis athletes and coaches in planning their training protocols and techniques.
Which is more faithful, seeing or saying? Multimodal sarcasm detection exploiting contrasting sentiment knowledge
Authors: Chen Yutao , Shi Shumin , Huang Heyan 
Published: December 2024

AbstractUsing sarcasm on social media platforms to express negative opinions towards a person or object has become increasingly common. However, detecting sarcasm in various forms of communication can be difficult due to conflicting sentiments. In this paper, we introduce a contrasting sentiment‐based model for multimodal sarcasm detection (CS4MSD), which identifies inconsistent emotions by leveraging the CLIP knowledge module to produce sentiment features in both text and image. Then, five external sentiments are introduced to prompt the model learning sentimental preferences among modalities. Furthermore, we highlight the importance of verbal descriptions embedded in illustrations and incorporate additional knowledge‐sharing modules to fuse such image‐like features. Experimental results demonstrate that our model achieves state‐of‐the‐art performance on the public multimodal sarcasm dataset.
Mechanism and suppression of breaking overvoltage of DC circuit breakers
Authors: Qu Lu , Yu Zhangqing , Gan Zhizheng , Yan Xin , Jiang Cheng , Huang Yulong , Li Mingshen , Feng Jian 
Published: December 2024

AbstractThe occurrence of breaking overvoltage in a DC circuit breaker (DCCB) poses a potential threat to the safe operation of a DC grid. Based on the structure of the ±10 kV three‐terminal DC distribution network, this paper sets up the medium‐voltage DCCB and formulates a fault protection strategy. Additionally, a simulation model of the system is developed to analyse the overvoltage characteristics within the DC distribution network across different nodes and fault conditions. By examining the factors that influence breaking overvoltage, this paper unravels the fundamental mechanisms associated with DCCB‐related overvoltage generation. Furthermore, the paper suggests measures to alleviate DCCB overvoltage. These insights provide a theoretical and technical basis for the design and operation of DCCBs within DC distribution networks.
An approach to distributed asynchronous multi‐sensor fusion utilising data compensation algorithm
Authors: Wang Kuiwu , Zhang Qin , Jin Zhenlu , Fu Xiaolong 
Published: December 2024

AbstractMulti‐sensor networks often encounter challenges such as inconsistent sampling times among local sensors and data loss during transmission. To address these issues, this paper employs a data loss compensation strategy to reconstruct missing data information. It designs the state estimation of local sensors utilising iterative state equations, leveraging multistep prediction techniques to estimate sensor states at unsampled points, thereby transforming the asynchronous sensor network system into a synchronous one. Furthermore, the projection theorem is applied to determine the fusion weights of local sensors, grounded on the principle of square‐averaging significance. Ultimately, data information pertaining to the same target is fused through arithmetic averaging, guided by distance correlation. Simulation outcomes demonstrate that the proposed algorithm balances estimation accuracy with communication overhead, achieved by designing an optimal number of communication iterations.
CircTLK1 Knockdown Alleviates Cell Inflammation and Apoptosis by Regulating Elavl1/Nox4 Axis in Neonatal Sepsis-Induced Lung Injury in Mice
Authors: Li Hongxia , Li Jiansheng , Zhang Jin 
Published: December 2024

Objective Septic acute lung injury (ALI) is a common complication of sepsis with high morbidity and mortality but lacks specific treatment. This study aimed to elucidate the role of circular RNA TLK1 (circTLK1) in neonatal septic ALI.
Study Design Murine cecal slurry was used to induce neonatal sepsis-induced ALI model in vivo. Hematoxylin and eosin staining was performed to detect the pathological changes in lung tissues. Pulmonary microvascular endothelial cells were treated with lipopolysaccharide (LPS) to induce neonatal sepsis-induced ALI model in vitro. The levels of IL-1β and IL-6 were detected by enzyme-linked immunosorbent assay. A lactate dehydrogenase (LDH) detection kit was used to detect LDH activity. Cell Counting Kit-8 assay and flow cytometry detected cell viability and apoptosis. The genes' expression was measured by quantitative real-time reverse-transcription polymerase chain reaction and western blot. The relationship between circTLK1 and Elavl1 or Elavl1 and Nox4 was detected using RNA immunoprecipitation assay.
Results Our results illustrated that circTLK1 was highly expressed in neonatal sepsis-induced ALI model, and circTLK1 knockdown alleviated cell inflammation and apoptosis in neonatal sepsis-induced ALI model. Similarly, we found that circTLK1 knockdown alleviated neonatal sepsis-induced ALI. Mechanically, circTLK1 mediated Elavl1 binding to Nox4 messenger RNA and increased its stability. Functionally, circTLK1 knockdown alleviated cell inflammation and apoptosis by regulating Nox4 in the neonatal sepsis-induced ALI model.
Conclusion CircTLK1 knockdown alleviated cell inflammation and apoptosis by the Elavl1/Nox4 axis in neonatal sepsis-induced ALI. Our research provided a novel direction for the treatment of neonatal sepsis-induced ALI.
Key Points
Straightforward Synthesis of Unnatural α,α-Disubstituted α-Amino Acid Derivatives Using Readily Accessible Building Blocks
Authors: Tomohara Keisuke , Nose Takeru , Nambu Hisanori 
Published: December 2024

AbstractUnnatural α,α-disubstituted α-amino acids have played a privileged role in drug discovery campaigns, and the development of their efficient synthetic methods has thus received continuous attention. This account describes our originally developed synthetic methods to obtain α,α-disubstituted α-amino acid derivatives using readily accessible building blocks.1	Introduction2	Asymmetric Synthesis of α,α-Disubstituted α-Aryl α-Amino Acid
Derivatives via ‘Memory of Chirality’3	Synthesis of Natural-Product-Derived α,α-Disubstituted α-Amino Acid Derivatives by Chemical Engineering of Natural Product Extracts4	One-Pot Synthesis of α,α-Disubstituted α-Amino Acid Derivatives by Ammonia-Ugi Reaction5	Conclusions
Má consolidação em fratura autoestabilizada de tipo II do processo odontoide com subluxação atlantoaxial anterior crônica em paciente neurologicamente intacto: Relato de caso
Authors: Gomez-Rios Juan Carlos , Uribe-Alpizar Claudio , Reyes-Sanchez Alejandro , García-Ramos Carla Lisette , Rosales-Olivarez Luis Miguel , Zárate-Kalfópulos Baron 
Published: December 2024

ResumoAs luxações atlanto-occipitais com fraturas de tipo II do processo odontoide são raras, com relato de sete casos a cada 784 lesões da coluna cervical superior. Sua incidência é inferior a 0,3% e estas lesões estão relacionadas a uma alta taxa de morbidade e mortalidade. As fraturas de C2 mais comuns são no processo odontoide, representando 7% dos casos. Essas fraturas são classificadas por Anderson e D'Alonso de acordo com seu nível e o maior índice de pseudoartrose foi observado na zona II. Até 85% dessas lesões são causadas por acidentes automobilísticos. Não há consenso quanto ao tratamento ideal dos casos agudos. Realizamos anamnese completa e exame físico em nossa instituição. Fizemos uma revisão sistemática de relatos de casos com as palavras-chave “malunion of odontoid process + chronic atlantoaxial subluxation” (má consolidação do processo odontoide + subluxação atlantoaxial crônica) em quatro bases de dados diferentes e uma análise comparativa dos casos encontrados. Não observamos casos idênticos ao do nosso relato. Nove relatos de casos semelhantes foram publicados; as principais diferenças foram a pseudoartrose do processo odontoide, as alterações neurológicas em pacientes sintomáticos e o consequente tratamento cirúrgico.É raro observar um paciente com fratura do processo odontoide de tipo II, consolidação viciosa e subluxação atlantoaxial anterior estável sem alterações neurológicas. Decidimos pela conduta expectante com acompanhamento anual.
Resultados a longo prazo após 18 anos de fixação artroscópica de fratura articular da escápula: Relato de caso
Authors: Ramos Carlos Henrique , Barbosa Rafaella Monteiro , Gomes Yasmin Netto Costa , Paula Ana Luisa Garcia de , Gulicz Laysla Danyela Coradin 
Published: December 2024

ResumoA redução e fixação das fraturas articulares da cavidade glenoidal pela técnica artroscópica causam pouco trauma cirúrgico, possibilitando o diagnóstico complementar e tratamento de eventuais lesões associadas (capsulares, ligamentares e/ou tendinosas), sendo demonstrados resultados promissores até o momento. Os autores relatam um caso de fratura glenoidal tipo III de Ideberg, associada à fratura da clavícula distal, submetida a redução e fixação óssea percutânea (fios de K) assistida pela técnica artroscópica. Descrevem a técnica e o resultado após 18 anos de acompanhamento, com a avaliação clínica realizada segundo critérios funcionais do score University of California at Los Angeles (UCLA) e o estudo radiográfico. O resultado foi considerado excelente/satisfatório, com paciente assintomático ao longo do tempo e ausência de alterações radiográficas relevantes. Apesar do manejo de fraturas da glenoide pela artroscopia ainda estar em evolução, apresenta-se como boa alternativa de tratamento em relação à abordagem aberta, especialmente nas fraturas de menor complexidade.
Minimally Invasive Placement of Cannulated Headless Compression Screws for Reduction of Sacroiliac Luxation in 14 Cats
Authors: de Jong Lars , Proot Joachim Lucas Jozef , Pillin Lorenzo Josepha Paul , Janssens Luc August Adèle 
Published: December 2024

Abstract
Objective To describe percutaneous fluoroscopy-guided placement of self-drilling, self-tapping, 3.0 mm cannulated headless compression screws (HCS) for surgical reduction of sacroiliac luxation (SIL) in cats, and to document clinical outcome.
Materials and Methods Medical records of cats with SIL, managed by percutaneous fluoroscopy-guided placement of a 3.0 mm HCS, were reviewed. Short-term outcome was assessed by clinical examination and radiographs. Long-term outcome was assessed using an owner questionnaire.
Results A total of 14 cats, with 17 SIL (11 unilateral and 3 bilateral), were included. Mean postoperative sacroiliac joint reduction was 85%, median sacral purchase was 63% for unilateral and 38% for bilateral HCS, and pelvic canal diameter ratio and hemipelvic canal width ratio were satisfactory in all cases. At follow-up, all cats exhibited satisfactory clinical improvement. Radiographic assessment revealed no implant-related complications of the unilateral HCS; however, screw loosening was observed in 3/6 of bilateral HCS. Owner satisfaction was good, with 10/11 of owners being very satisfied and 1/11 being satisfied.
Clinical Significance Percutaneous fluoroscopy-guided placement of a 3.0 mm HCS is a safe and effective method for unilateral surgical reduction of SIL in cats. Further studies are needed to establish the most appropriate procedure to treat bilateral SIL.
Von Meyerberg Complexes: An Underrecognized Predisposing Factor for Focal Nodular Hyperplasia–Like Lesions
Authors: Rastogi Ruchi , Aggarwal Bharat , Gupta Subhash , Agarwal Komal , Chugh Bhuvan 
Published: December 2024

AbstractVon Meyerberg complexes (VMCs), also termed as biliary hamartomas, are common ductal plate malformations and are considered as benign incidental findings. These are occasionally discovered in histopathological examination or on imaging in individuals being investigated for an unrelated issue and do not warrant any active intervention. However, it is important to recognize that sometimes VMCs can predispose to malignant masses or rarely to hypervascular benign masses as in present case, causing diagnostic dilemma.
Leaf Na+ effects and multi-trait GWAS point to salt exclusion as the key mechanism for reproductive stage salinity tolerance in rice
Authors: de Ocampo Marjorie P , Tam Bui Phuoc , Egdane James A , Chebotarov Dmytro , Doi Kazuyuki , Yamauchi Akira , Ismail Abdelbagi M , Henry Amelia , Mitsuya Shiro 
Published: December 2024

Abstract

Background and Aims
Since salinity stress may occur across stages of rice (Oryza sativa) crop growth, understanding the effects of salinity at the reproductive stage is important, although it has been much less studied than at the seedling stage.

Methods
Lines from the Rice Diversity Panel 1 (RDP1) and the 3000 Rice Genomes (3KRG) were used to screen morphological and physiological traits, map loci controlling salinity tolerance through genome-wide association studies (GWAS), and identify favourable haplotypes associated with reproductive stage salinity tolerance.

Key Results
Salt exclusion was identified as the key tolerance mechanism in this study, based on reduced panicle length as flag leaf Na+ increased and a lack of effect of trimming the leaves on genotypic rankings in the salinity treatment. Since larger biomass showed a negative effect on the number of filled grains in multiple experiments, future studies should investigate the effect of whole-plant transpiration levels on salt uptake. In addition to genome-wide significant peaks identified in the single-trait GWAS analyses, six loci showed colocations for multiple traits across experiments. Among these colocating loci, three candidate loci that exhibited favourable haplotypes were also characterized to be involved in co-expression networks, among which apoplast and cell wall functions had been annotated, further highlighting the role of salt exclusion.

Conclusion
The loci identified here could be considered as potential sources for improving reproductive stage salinity tolerance in rice.
Nanovesicles derived from edible plants: a new player that contributes to the function of foods
Authors: Yamasaki Masao , Yamasaki Yumi , Oshima Tatsuya 
Published: December 2024

Abstract
Nano-sized vesicles are ubiquitous in vegetables, fruits, and other edible plants. We have successfully prepared nanovesicles (NVs) from over 150 edible plants. These results suggest that the daily intake of NVs from various foods and their roles in food function are promising novel approaches for explaining the health-promoting properties of edible plants. These vesicles contain RNAs, including miRNAs, similar to extracellular NVs, which play pivotal roles in cell-cell communication. Intriguingly, NVs also contain phytochemicals such as polyphenols and carotenoids that are specific to each edible plant. In conclusion, these dietary NVs have the potential to serve as functional packages to deliver RNAs or phytochemicals to target cells across species from plants to humans.
VarNMF: Non-negative Probabilistic Factorization with Source Variation
Authors: Fallik Ela , Friedman Nir 
Published: December 2024

Abstract

Motivation
Non-negative Matrix Factorization (NMF) is a powerful tool often applied to genomic data, to identify non-negative latent components that constitute linearly mixed samples. It is useful when the observed signal combines contributions from multiple sources, such as cell types in bulk measurements of heterogeneous tissue. NMF accounts for two types of variation between samples—disparities in the proportions of sources and observation noise. However, in many settings, there is also a non-trivial variation between samples in the contribution of each source to the mixed samples.

Results
We present VarNMF, a probabilistic extension of NMF that explicitly models this source variation. We show that by modeling sources as non-negative distributions, we can recover source variation directly from mixed samples without observing any of the sources directly. We apply VarNMF to a cell-free ChIP-seq dataset of two cancer cohorts and a healthy cohort, demonstrating that VarNMF provides a better estimation of the data distribution. Moreover, VarNMF extracts cancer-associated source distributions that decouple the tumor characteristics from the amount of tumor contribution, and identify patient-specific disease behaviors. This decomposition highlights the inter-tumor variability that is obscured in the mixed samples.

Availability
Code is available at https://github.com/Nir-Friedman-Lab/VarNMF. cfChIP data is based on Sadeh et al. (2021) (publicly available data) and Fialkoff et al. (2022) (provided through authors).

Supplementary information
Supplementary data are available at Journal Name online.