bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Analysis of Key Genes Associated with Ulcerative Colitis by Integrated Bioinformatics Methods
Authors: Yu Xingyu , Li Jinjie , Chen Hongci , Chen Xingmeng , Xiang Yu 
Published: November 2021

Abstract
Background: Ulcerative colitis (UC) is a prevalent inflammatory bowel disease of the colonic mucosa. The exact mechanism of the disease still remains unclear. Here we tried to explore new biomarkers and potential therapeutic targets in UC through adopting integrated bioinformatics tools.Results: By performing DEGs analysis, 59 upregulated and 39 downregulated DEGs were successfully identified from GSE3365, respectively. And they were mainly enriched in the terms of Cytokine-cytokine receptor interaction,Viral protein interaction with cytokine and cytokine receptor,Pantothenate and CoA biosynthesis,IL-17 signaling pathway and Chemokine signaling pathway. Based on the data of protein–protein interaction (PPI), the top 10 hub genes were ranked, including Growth-regulated alpha protein (CXCL1), C-C motif chemokine 2 (CCL2), C-X-C chemokine receptor type 1 (CXCR1), Low affinity immunoglobulin gamma Fc region receptor III-B (FCGR3B), C-X-C chemokine receptor type 2 (CXCR2), Prostaglandin G/H synthase 2 (PTGS2), Triggering receptor expressed on myeloid cells 1 (TREM1), Interleukin-1 receptor type 1 (IL1R1), fMet-Leu-Phe receptor (FPR1), and Band 3 anion transport protein (SLC4A1).What’s more, the results of correlation analysis demonstrated that there was a positive correlation between the 10 hub DEGs.Conclusion: Ten DEGs were identified as potential candidate diagnostic biomarkers for patients with UC in present study. However, further experiments are needed to confirm the functional pathways and hub genes associated with UC.
Programmed Death-1 Deficiency Aggravates Motor Dysfunction In MPTP Model of Parkinson's Disease By Inducing Microglial Activation And Neuroinflammation In Mice
Authors: Cheng Ying-Ying , Chen Bei-Yu , Bian Gan-Lan , Ding Yin-Xiu , Chen Liang-Wei 
Published: November 2021

Abstract
Abundant reactive gliosis and neuroinflammation are typical pathogenetic hallmarks of brains in Parkinson’s disease (PD) patients, but regulation mechanisms are poorly understood. We are interested in role of programmed death-1 (PD-1) in glial reaction, neuroinflammation and neuronal injury in PD pathogenesis. Using PD mouse model and PD-1 knockout (KO) mice, we designed wild-type-control (WT-CON), WT-1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (WT-MPTP), PD-1-KO-control (KO-CON) and PD-1-KO-MPTP (KO-MPTP), and observed motor dysfunction of animal, morphological distribution of PD-1-positive cells, dopaminergic neuronal injury, glial activation and generation of inflammatory cytokines in midbrains by motor behavior detection, immunohistochemistry and western blot. WT-MPTP mouse model exhibited decrease of PD-1/Iba1-positive microglial cells in the substantia nigra compared with WT-CON mice. By comparison of four groups, PD-1 deficiency showed exacerbation in motor dysfunction of animals, decreased expression of TH protein and TH-positive neuronal protrusions. PD-1 deficiency enhanced microglial activation, production of pro-inflammatory cytokines like inducible nitric oxide synthase, tumor necrosis factor-α, interleukin-1β and interleukin-6, and expression and phosphorylation of AKT and ERK1/2 in the substantia nigra of MPTP model. We concluded that PD-1 deficiency could aggravate motor dysfunction of MPTP mouse model by inducing microglial activation and neuroinflammation in midbrains, suggesting that PD-1 signaling abnormality might be possibly involved in PD pathogenesis.
Hereditary Spherocytosis Caused by a Novel Compound Heterozygous Mutation of the SPTA1 Gene and Autoimmune Hepatitis in a Pediatric Patient
Authors: Qin Yu-mei , Chen Yan-yun , Liao Lin , Wu Yang-yang , Chen Min , Lin Fa-quan 
Published: November 2021

Abstract
Objective: Patients suffering from both hereditary spherocytosis (HS) and autoimmune hepatitis (AIH) are very rare. We analyzed the clinical and genetic characteristics of a seven-year-old girl with yellow sclerae and abnormal liver function tests, but no further symptoms. Methods: Blood samples were collected from the proband, her parents, and her paternal grandmother, and analyzed using routine laboratory tests, as well as subjected to next-generation and Sanger sequencing.Results: Compound heterozygous mutations of the spectrin alpha, erythrocytic 1 (SPTA1) gene were identified in the proband. Thec.134G>A (p.R45K) and c.6544G>C (p.D2182H) mutations were inherited from her mother and father, respectively. The proband’s father and paternal grandmother had the same mutation. Neither mutation is described in the Human Gene Mutation Database. Conclusions: HS has clinical manifestations similar to AIH, it may be difficult to diagnose when it coexists with AIH. When laboratory results cannot be explained by autoimmune liver disease alone, the possibility of a concomitant disease should be considered. Pedigree investigation and genetic analyses might be required to arrive at the final diagnosis.
ICU Mortality is Increased in Septic Shock Patients Accompanied with Hypo- or Hyper- Serum Osmolarity: A Retrospective Study
Authors: Heng Gang , Zhang Jiasi , Huang Benqi , Shen Yanbing , Li Zhonghu , Jia Jiankun , Zhang Chengcheng , Zhang Jianxin , Fu Tao , Jin Weidong 
Published: November 2021

Abstract
Purpose: While many factors that are associated with increased mortality in septic shock patients have been identified, the effect of serum osmolarity to altering patient outcomes among ICU patients with septic shock has not been studied. This study was designed to examine the association of serum osmolarity with ICU 28-day mortality in that population. Methods: The MIMIC-IV database was employed to identify patients diagnosed with septic shock. The serum osmolarity was calculated according to the serum concentration of Na + , K + , glucose and urea nitrogen synchronously. The statistical approaches used included multivariate logistic regression, propensity score analysis, inverse probability-weighting and causal mediation analysis. Results: In this study, significant difference of 28-day mortality was observed in septic shock patients accompanied with hypo-osmolarity, hyper-osmolarity and normal osmolarity (30.8%, 35.0% and 23.0%, P<0.001) which was detected at ICU admission. We also found that transforming the hyper-osmolarity into normal osmolarity by fluid therapy in day 2 and day 3 would decrease this mortality. Conclusion: Serum abnormality is significantly associated with increased 28-day mortality in septic shock patients.
Water Dynamics in the Understory of a Pine Plantation Forest After Variable Retention Harvesting
Authors: Bodo Alanna V , Arain M. Altaf 
Published: November 2021

Abstract
Background: Variable Retention Harvesting (VRH) is a silvicultural technique applied to enhance forest growth, and restore forest stands to closely resemble their natural compositions. This study used sapflow and understory eddy covariance flux measurements to examine the impacts of four different VRH treatments on the dominant components of evapotranspiration including canopy transpiration and water flux from understory vegetation and soil. These VRH treatments were applied to an 83-year-old red pine (Pinus resinosa) plantation forest in the Great Lakes region in Canada and included 55% aggregated crown retention (55A), 55% dispersed crown retention (55D), 33% aggregated crown retention (33A), 33% dispersed crown retention (33D) and unharvested control (CN) plot. Results: Study results showed a positive relationship between thinning intensity and the growth of understory vegetation, and hence enhanced evapotranspiration. The contribution to evapotranspiration from understory vegetation and soil was more pronounced in the dispersed thinning treatments, as compared to the aggregated. Overall, canopy transpiration contributed to 83% of total evapotranspiration in the un-thinned control plot and 55, 58, 30, and 23% for the 55A, 55D, 33A and 33D plots, respectively. The thinning or retention harvesting enhanced the water use efficiency in all treatments.Conclusion: Our results suggest VRH treatments that follow a dispersed harvesting pattern may provide the optimal balance between forest productivity and evapotranspiration or stand water use. Furthermore, a balance of contributions from both the canopy and successional understory vegetation and soil, as observed in the 55% retention harvesting treatment, may increase the resiliency of forest to climate change. These findings will help researchers, forest managers and decision-makers to improve their understanding of thinning impacts on water and carbon exchanges in forest ecosystems and adopt appropriate forest management practices to enhance their carbon sequestration capabilities, water use efficiency and resilience to climate change.
Immune Activation and Inflammatory Biomarkers as Predictors of Venous Thromboembolism in Lymphoma Patients
Authors: Otasevic Vladimir , Mihaljevic Biljana , Milic Natasa , Stanisavljevic Dejana , Vukovic Vojin , Tomic Kristina , Fareed Jawed , Antic Darko 
Published: November 2021

Abstract
Background: Lymphomas are characterized by inflammatory soluble mediators that can trigger the development of venous thromboembolism (VTE). However, data on the relationship between specific immune dysregulation and VTE occurrence in lymphoma are scarce. The study aimed to assess the association between inflammatory markers and the risk of VTE development in lymphoma patients.Methods: The erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lactate dehydrogenase (LDH), total protein (TP), and albumin were assessed in 706 patients with newly diagnosed or relapsed lymphoma. Data were collected for all VTE events, while the diagnosis of VTE was set objectively based on radiographic studies. ROC (receiver operating characteristic) curve analysis was used to define the optimal cutoff values for predicting VTE.Results: The majority of patients were diagnosed with aggressive non-Hodgkin lymphoma (58.8%) and had advanced disease (59.9%). Sixty-nine patients (9.8%) developed VTE. The NLR, PLR, ESR, CRP, and LDH were significantly higher in lymphoma patients with VTE, whereas the TP and albumin were significantly lower. In the univariate regression analysis, the NLR, PLR, TP, albumin, LDH, and CRP were prognostic factors for VTE development. In the multivariate regression model, the NLR and CRP were independent prognostic factors for VTE development. ROC curve analysis demonstrated acceptable specificity and sensitivity of the NLR, PLR, and CRP for predicting VTE. Conclusion: Inflammatory dysregulation plays an important role in VTE development in patients with lymphoma. Widely accessible, simple inflammatory parameters can classify lymphoma patients at risk of VTE development.
Evaluating Water-Yield Property of Karst Aquifer Based On The AHP And CV
Authors: Yu Shuai , Ding Hanghang , Zeng Yifan 
Published: November 2021

Abstract
In order to ensure the safety of mine production, it is of great practical significance to make a reasonable evaluation of the water-yield property (WYP) of a karst aquifer. In this paper, we selected fault-lines distribution, fault-scale index, aquifer thickness, water pressure, consumption of rinsing liquid, and hydraulic conductivity as the evaluation indexes to analyze the WYP of a karst aquifer. Meanwhile, the analytic hierarchy process (AHP) is used to calculate the subjective weight of indexes, and the coefficient of variation (CV) is used to calculate the objective weight of indexes. Combined with GIS, a multi-factor composite superposition is carried out to evaluate the WYP of a karst aquifer. The reliability of the research results is verified by the specific yield. Besides, for improving the reliability of evaluation results, the chemical composition of karst water was discussed. The results show that the selection of indexes is reasonable and the AHP-CV method is effective to evaluate the WYP of a karst aquifer. Therefore, on the premise of reasonable index selection, the evaluation models of AHP and CV can be used to evaluate the WYP of a karst aquifer and provide reference for coal mine water control measures.
BNDNN: Batch Normalization Based Deep Neural Network for Predicting Flood in Urban Areas
Authors: Dubey Vinay , Katarya Rahul 
Published: November 2021

Abstract
Disaster is a very serious dissipation that arises for a short time period, but the impact of that disaster on human society is very dangerous and very long-lasting. Disasters are categorized into two types like natural disasters and manmade disasters. Among all disasters, of all the natural disasters, flood is the commonplace natural disaster. Flood disaster that causes huge loss of human life, diversity as well as economic loss, which is very dangerous for the developing countries and developed countries also. Nowadays during the monsoon season flood is dangerous for all the geographical areas located nearby water bodies. Much research has been done for flood detection. Machine Learning and many other recent technologies are playing a vital role in predicting the occurrence of floods. For prediction purposes, a huge amount of data is requiring collected from sensors deployed in various locations. In this paper, we used the Batch normalization with Deep Neural Network (BNDNN) technique for the classification of data in three classes as Low, Moderate, and High. The result obtained from our proposed model is compared with some other models like Decision Tree (DT), Support Vector Machine (SVM), Artificial Neural Network (ANN), and Deep Neural Network (DNN). In this our proposed BNDNN provides 89% accuracy which is higher among all existing models. Models are compared based on some parameters like Accuracy, Precision, Recall, F –Score. The compression among all the models used in this paper shows that our proposed model provides better results.
Maternal Exposure to Local, Source-specific Ambient Air Pollution During Pregnancy and Autism in Children: a Cohort Study From Southern Sweden
Authors: Flanagan Erin , Malmqvist Ebba , Rittner Ralf , Gustafsson Peik , Källén Karin , Oudin Anna 
Published: November 2021

Abstract
Evidence of air pollution exposure, namely, ambient particulate matter (PM), during pregnancy and an increased risk of autism spectrum disorders (ASD) in children is growing. Which PM sources contribute to this association is currently unknown, however. The aim of the present study was to investigate local source-specific ambient PM exposure and its association with ASD. A cohort of 48,571 singleton births from 1999 to 2009 in Scania, Sweden, was combined with data on locally emitted PM with an aerodynamic diameter <2.5 µm (PM2.5). A flat, two-dimensional dispersion model was used to model PM2.5 exposure (all-source PM2.5, tailpipe exhaust, vehicle wear-and-tear, and small-scale residential heating- mainly wood burning) at the residential address. Associations were analyzed using binary logistic regression in partially and fully adjusted models. Clear associations were observed between PM2.5 and ASD, with statistical significance for all investigated sources in the partially adjusted model. In the fully adjusted model, only all-source PM2.5 was statistically significant. The results add to existing evidence that exposure to air pollution during pregnancy may be associated with an increased risk for ASD among children. Further, these findings suggest that locally emitted tailpipe exhaust, vehicle wear-and-tear, and small-scale residential heating all contribute to this association.
The Effect of 2-Mercaptobenzimidazole-Polyaniline-CeO2 Ternary Nanocomposite Addition as a Superior Pigment for Improvement of Corrosion Resistance in Epoxy Coatings
Authors: Aboutalebi Khadijeh , Sefidi Pariya Yardani , Hosseini Mir ghasem , Kinayyigit Solen , Ghouchi Armin 
Published: November 2021

Abstract
The 2-mercaptobenzimidazole-polyaniline-ceria (MBI-PANI-CeO2) ternary nanocomposite synthesized and used as pigment into epoxy (EP) to improve protection properties of coating on mild steel. The MBI-PANI-CeO2 nanocomposite was prepared using layer by layer assembly. Initially, the PANI layer was polymerized on the surface of CeO2 nanoparticles. Then, the MBI inhibitor was adsorbed on PANI with opposite electrostatic charges. The anticorrosive performance of EP coatings was investigated using electrochemical impedance spectroscopy, salt spray test, and scanning electron microscopy by incorporating various amounts (0.5, 1, and 2 wt. %) of the MBI-PANI-CeO2 nanocomposite. The EP coating containing 1 wt. % of nanocomposite showed the highest corrosion resistance and minimum agglomeration. This coating indicated the coating resistance of 19.1 MΩ cm2, which is greater than EP and EP/CeO2 coatings. The EP/MBI-PANI-CeO2 (1 wt. %) coating showed water uptake percentage (2.09 %) about two times lower than EP/CeO2 coatings (4.56 wt. %), indicating appropriate barrier performance of inhibitor incorporated EP.
Haemospermia in the Real- Life Setting: a New High-Risk Stratification
Authors: Pozzi Edoardo , Ventimiglia Eugenio , Fallara Giuseppe , Capogrosso Paolo , Belladelli Federico , Candela Luigi , Raffo Massimiliano , Boeri Luca , Matloob Rayan , Capitanio Umberto , Montorsi Francesco , Salonia Andrea 
Published: November 2021

Abstract
We aimed to validate the EAU guidelines in a homogeneous cohort of men with haemospermia, and to identify a novel and better performing risk stratification compared to EAU guidelines. Data from 283 consecutive patients complaining of a single episode/recurrent haemospermia were retrospectively analysed. Patients were stratified into low vs. high-risk according to EAU guidelines, whose diagnostic performance was then validated. We identified a new risk stratification model based on clinical factors associated with i) positive semen culture and ii) prostate cancer (PCa) and bladder cancer (BC). Diagnostic accuracy of the two predictive models (EAU vs. New) was assessed and decision curve analyses (DCA) tested their clinical benefit. Overall, 259 (91.5%) were high-risk and 24 (8.5%) low risk according to the EAU guidelines. Recurrent haemospermia was reported by 134 (47.4%) patients. 126 (44.5%) had baseline CCI score ≥ 1. At MVA logistic regression analysis, history of recurrent genito – urinary tract infections was identified as a predictor for positive semen culture (OR: 3.39, 95% CI: 1.77 – 6.57, p=0.002). Likewise, baseline CCI ≥ 1 was identified as a predictor for PCa and BC (OR: 1.55, 95% CI: 1.17 – 2.04, p=0.009). Sensitivity, specificity, and AUC of the EAU guidelines were 13.3%, 89.2% and 51% respectively, whereas the new model performed substantially better: 98.9%, 58% and 78% respectively. The application of the EAU guidelines risk stratification does not ensure proper identification of high-risk patients complaining of haemospermia. We propose a novel, better performing and easily implementable risk stratification tool.
What are the Results of Resection of Forearm Soft Tissue Sarcoma?
Authors: Nakata Eiji , Fujiwara Tomohiro , Kunisada Toshiyuki , Nakahara Ryuichi , Watanabe Toshiyuki , Ozaki Toshifumi 
Published: November 2021

Abstract
Only few reports have assessed the characteristics and oncological and functional outcomes of forearm soft tissue sarcomas (STS). Then, we aimed to investigate the clinical features and survival-related factors for forearm STS who underwent surgical excision at our institution. There were 38 patients. Fourteen patients (41%) were referred to our institution after an unplanned excision and tumor size and grade were significantly associated with the receipt of it. The postoperative median Musculoskeletal Tumor Society rating scale (MSTS) score was 28. Bone resection or major nerve palsy was the only factor influencing the postoperative MSTS score (P < 0.001). There was no significant difference in MSTS scores according to the reconstruction procedures (the use of flap or tendon reconstruction). The 5-year local recurrence-free survival (LRFS) rate was 86%. Univariate analysis revealed that the histological diagnosis of myxofibrosarcoma was the only factor that influenced LRFS (P = 0.047). The 5-year metastasis-free survival rate was 77%. The 5-year overall survival (OS) rate was 94%. Age was the only factor that influenced OS (P = 0.01). In conclusion, reconstruction of the skin and tendon can compensate for function. Careful follow-up is important, especially in patients with myxofibrosarcoma, due to its likelihood of local recurrence.
Investigating Bangladeshi university students’ willingness to communicate (WTC) in English classes through a qualitative study
Authors: Alam Md Rabiul 
Published: November 2021

Abstract
In Bangladesh, learning English starts as a compulsory subject like other core subjects from the very early stage of a learner. However, after spending at least 12 years in learning English, learners’ oral skill is not seen up to the mark while they enter into the university for their tertiary education. Sometimes, they are noticed to have their unwillingness to communicate with others. Hence, the current study aims to see the experiences of Bangladeshi university students’ willingness to communicate (WTC) by employing a multiple case study method. 10 undergraduate students from a private university were interviewed. Apart from semi-structured interview, classroom observations were also carried out for data collection. The obtained data were analysed through the content analysis process. The findings show that students’ earlier experiences of learning English have been positive; however, they see grammar-based English learning as an obstacle in their communication development. It also reveals that learners’ WTC is greatly influenced by classroom environment that enhances or declines learners’ interest to communicate with others. Based on the findings, the study suggests incorporating more drills in English classes by engaging learners with real-life based conversations, dialogues and so forth to enhance learners’ WTC. Hence, learners’ real voice would help the policymakers and language instructors to take the issue into consideration and way-out timely measures. As the study has considered fewer respondents, future studies are expected to shed light on more respondents including the voice of the teachers to have a deeper understanding of the issue.