bluuebunny/crossref_metadata_2025_split huggingface dataset:
Not a Problem at All or Excluded by Oneself, Doctors and the Law? Healthcare Workers’ Perspectives on Access to HIV-Related Healthcare Among Same-Sex Attracted Men in Tanzania
Authors: Mwijage Alexander Ishungisa , Mizinduko Mucho Michael , Likindikoki Samuel Lazarus , Meyrowitsch Dan Dan Wolf , Mmbaga Elia John , Leshabari Melkizedeck Thomas , Moen Kåre 
Published: May 2021

Abstract
Background: Same-sex attracted men in Tanzania are disproportionately affected by HIV. Drawing on qualitative research, the present study explores healthcare providers’ perspectives on access to HIV-related healthcare services among same-sex attracted men. Methods: A qualitative study was carried out among healthcare workers in in the cities of Dar es Salaam and Tanga in Tanzania between August 2018 and October 2019. Fieldwork entailed qualitative interviewing, focus group discussions and participant observation. A purposive sampling strategy was used to select study participants who varied with respect to age, education level, work experience, and the type and location of the facilities they worked in. Twenty-four interviews and six focus group discussions were conducted.Results: This paper describes four different discourses that were identified among healthcare workers with respect to their perception of access to healthcare services for SSAM. One held that access to healthcare was not a major problem at all, another that some SSAM did not utilize healthcare services although they were available, a third that some healthcare workers prevented SSAM from gaining access to healthcare and a fourth that healthcare for gender and sexual minority persons was made difficult by structural barriers. Conclusion: Although these are four rather different takes on the prevailing circumstances with respect to healthcare access for SSAM, we suggest that they may all be ‘true’ in the sense that they grasp and highlight different aspects of the same realities.
Organosilane-Functionalized Graphene Oxide Hybrid Material: Efficient Adsorbent For Heavy Metal Ions In Drinking Water
Authors: KARABÖRK Muharrem , Muhammed Ban Abdullelah , TÜMER Mehmet , URUŞ SERHAN 
Published: May 2021

Abstract
Organosilane-functionalized and chemically activated graphene-supported Schiff base ligand were synthesized and characterized with XRD, SEM, EDX, TEM, UV.-Vis., FT-IR and TG/DTA techniques. In the synthesis process, graphene oxide was firstly obtained with Hummer’ s method and reacted with 3-(trimethoxysilyl)propylamine. The last, the amine-activated and organosilane containing GO was reacted with 3,5-di-tert-butylsalicylaldehyde resulting the hybrid material (M) having azomethine group. The material (M) was used as an adsorbent in order to remove the cobalt(II) and zinc(II) ions in drinking water and standard solutions. The adsorption capacity of the material (M) to cobalt(II) and zinc(II) ions was investigated by batch method. Moreover, the effect of pH, contact time, temperature, and concentration on the adsorption capacity of the material was also investigated. The enrichment factors were calculated as 441 and 675 for zinc(II) and cobalt(II), respectively. The recovery performance of the material was determined in 98.65-101.75% and 98.55-104.0% range for zinc(II) and cobalt(II) ions, respectively.
Vitamin D Status and Parathyroid Hormone Levels in Girls With Central Precocious Puberty
Authors: Durá-Travé Teodoro , Gallinas-Victoriano Fidel , Ahmed-Mohamed Lotfi , Moreno-González Paula , Urretavizcaya-Martinez María , Malumbres-Chacón María 
Published: May 2021

Abstract
The objective of this study was to analyze the vitamin D status and PTH levels in 6- to 8-years-old girls with central precocious puberty. A cross-sectional clinical and blood testing (calcium, phosphorus, 25(OH)D and PTH) were carried out in 78 girls with central precocious puberty (CPP group), aged 6.1-7.9 years. A control group was recruited (157 prepubertal girls, aged 6.1-8.2 years). The criteria of the US Endocrine Society were used for the definition of hypovitaminosis D. There were no significant differences in the vitamin D status between both groups. There were not significant differences in 25(OH)D levels between CPP (25.4±8.6 ng/mL) and control groups (28.2±7.4 ng/mL). In contrast, in CPP group PHT levels (44.8±16.3 pg/mL) were higher (p < 0.05) with respect to control group (31.0±11.9 ng/mL). In CPP group there was positive correlation (p < 0.05) between PTH levels and growth rate, bone age, basal estradiol, basal FSH, basal LH and LH peak. Conclusion: Vitamin D status in 6- to 8-years-old girls with CPP is similar to that in prepubertal girls. PTH levels were significantly higher in girls with CPP, and could be considered as a pubertal characteristic and, in this case, of pubertal precocity.
Association Between Serum Sphingolipids and Eudaimonic Well-Being in White U.S. Adults
Authors: Berkowitz Loni , Henríquez Marcela P. , Salazar Cristian , Rojas Eric , Echeverría Guadalupe , Love Gayle D. , Rigotti Attilio , Coe Christopher L. , Ryff Carol D. 
Published: May 2021

Abstract
Emerging research has linked psychological well-being with many physiological markers as well as morbidity and mortality. In this analysis, the relationship between components of eudaimonic well-being and serum sphingolipids levels was investigated using data from a large national survey of middle-aged American adults (Midlife in the United States). Health behaviors (i.e., diet, exercise, and sleep) were also examined as potential mediators of these relationships. Serum levels of total ceramides - the main molecular class of sphingolipids previously associated with several disease conditions - were inversely linked with environmental mastery. In addition, significant correlations were found between specific ceramide, dihydroceramide, and hexosylceramides species with environmental mastery, purpose in life, and self-acceptance. Using hierarchical regression and mediation analyses, health behaviors appeared to mediate these associations. However, the link between ceramides and environmental mastery was partially independent of health behaviors, suggesting the role of additional mediating factors. These findings point to sphingolipid metabolism as a novel pathway of health benefits associated with psychological well-being. In particular, having a sense of environmental mastery may promote restorative behaviors and benefit health via improved blood sphingolipid profiles.
Diagnostic Performance of Unenhanced Electrocardiogram-gated Cardiac CT for Myocardial Edema Using Cardiac T2 Mapping as a Reference Standard
Authors: Emoto Takafumi , Kidoh Masafumi , Oda Seitaro , Sakabe Daisuke , Morita Kosuke , Hatemura Masahiro , Nakaura Takeshi , Nagayama Yasunori , Sasao Akira , Inoue Taihei , Funama Yoshinori , Takashio Seiji , Kaikita Koichi , Tsujita Kenichi , Hirai Toshinori 
Published: May 2021

Abstract
Purpose

To assess the diagnostic performance of unenhanced electrocardiogram (ECG)-gated cardiac CT for myocardial edema, using T2 mapping as the reference standard.
Methods

This study included 34 patients (23 men; age 64.7 ± 14.6 years) who underwent cardiac CT and MRI. On the unenhanced ECG-gated cardiac CT images, regions of interest (ROIs) were drawn on the septal myocardium. Using T2 mapping as the reference standard, the diagnostic performance of unenhanced cardiac CT for myocardial edema was evaluated by using the area under the receiver operating characteristic curve (AUC) with sensitivity and specificity.
Results

Mean CT values moderately correlated with mean T2 values (Rho = -0.41; P < 0.05). Mean CT values provided a sensitivity of 63.2% and a specificity of 93.3% for detecting myocardial edema, with a cut-off value of ≤ 45.0 HU (AUC = 0.77; P < 0.01). Inter-observer reproducibility in measuring mean CT values was excellent (ICC = 0.93; [95% CI: 0.86, 0.96]).
Conclusion

This is the first clinical study to assess the diagnostic performance of unenhanced ECG-gated cardiac CT for myocardial edema, using T2 mapping as the reference standard. Our study suggested that the CT value of myocardium in unenhanced ECG-gated cardiac CT may be an optional parameter in addition to late iodine enhancement and extracellular volume fraction for myocardial tissue characterization, but further prospective, large-scale clinical studies are needed.
Identification of Seven Novel Ferroptosis-Related Long Non - Coding RNA Signatures as a Diagnostic Biomarker for Acute Myeloid Leukemia
Authors: Zheng Zhiyuan , Wu Wei , Lin Zehang , Liu Shuhan , Chen Qiaoqian , Jiang Xiandong , Xue Yan , Lin Donghong 
Published: May 2021

Abstract
Background: Ferroptosis is a newly discovered type of programmed cell death that participates in the biological processes of various cancers. However, the mechanism by which ferroptosis modulates acute myeloid leukemia (AML) remains unclear. This study aimed to investigate the role of ferroptosis-related long non-coding RNAs (lncRNAs) in AML and establish a corresponding prognostic model.Methods: RNA-sequencing data and clinicopathological characteristics were obtained from The Cancer Genome Atlas database, and ferroptosis-related genes were obtained from the FerrDb database. The “limma” R package, Cox regression, and the least absolute shrinkage and selection operator were used to determine the ferroptosis-related lncRNA signature with the lowest Akaike information criteria (AIC). The risk score of ferroptosis-related lncRNAs was calculated and patients with AML were divided into high- and low-risk groups based on the median risk score. The Kaplan-Meier curve and Cox regression were used to evaluate the prognostic value of the risk score. Finally, gene set enrichment analysis (GSEA) and single-sample gene set enrichment analysis (ssGSEA) were performed to explore the biological functions of the ferroptosis-related lncRNAs.Results: Seven ferroptosis-related lncRNA signatures were identified in the training group, and Kaplan-Meier and Cox regression analyses confirmed that risk scores were independent prognostic predictors of AML in both the training and validation groups (All P < 0.05). In addition, the area under the curve (AUC) analysis confirmed that the signatures had a good predictive ability for the prognosis of AML. GSEA and ssGSEA showed that the seven ferroptosis-related lncRNAs were related to glutathione metabolism and tumor immunity.Conclusions: In this study, seven novel ferroptosis-related lncRNA signatures (AP001266.2, AC133961.1, AF064858.3, AC007383.2, AC008906.1, AC026771.1, and KIF26B-AS1) were established. These signatures were shown to accurately predict the prognosis of AML, which would provide new insights into strategies for the development of new AML therapies.
Rapid Detection of Pesticide in Milk, Cereal and Cereal based Food and Fruit Juices using Paper Strip-based Sensor
Authors: Dasriya Vaishali , Joshi Ritu , Ranveer Soniya , Dhundale Vishal , Kumar Naresh , VISHWESWARAIAH Raghu 
Published: May 2021

Abstract
The study was aimed to validate paper strip sensors for the detection of pesticide residues in milk, cereal-based food, and fruit juices in comparison with GC-MS/MS under field conditions. The detection limit of pesticide using rapid paper strip sensor for organophosphate, carbamate, organochlorine, fungicide, and herbicide group ranges from 1 to 10, 1–50, 250–500, 1–50, and 1 ppb, respectively in milk and milk product, cereal-based food and fruit juices. Among 125 samples of milk samples collected from the market 33 milk samples comprising 31 raw milk and 2 pasteurized milk found positive for pesticide using the strip-based sensor. In Cereal based food and fruit juice samples, 6 cereal flours and 4 fruit juices were found positive for pesticide residues. The pesticide positive samples were further evaluated quantitatively using GC-MS/MS wherein 7 samples comprised of raw milk, pasteurized milk, rice flour, wheat flour, maize flour, apple juice, and pomegranate juice have shown the presence of chlorpyrifos, chlorpyrifos-methyl, α-endosulfan, β-endosulfan DDD and DDT at trace level as well as at above MRL level. It is envisaged that the developed paper strip sensor can be a potential tool in the rapid and cost-effective screening of a large number of food samples for pesticide residues.
The clinicopathological and prognostic characteristics of mucinous micropapillary carcinoma of the breast
Authors: sun yangyang , gu wenxian , wang gengfang , zhou xiaoli 
Published: May 2021

Abstract
Background

Mucinous micropapillary carcinoma (MMPC) is a unique subtype of breast cancer and detailed investigation of clinical characteristics of MMPC is not fully investigated.
Methods

MMPC, pure mucinous breast carcinoma (PMBC) and invasive micropapollary carcinoma (IMPC) samples were enrolled simultaneously and performed immunohistochemistry (IHC) analysis to explore the clinicopathological attributes of MMPC. Moreover, survival analysis of MMPC were performed among MMPC, PMBC and IMPC group and within MMPC group.
Results

The result showed that MMPC demonstrated distinct pathological features and vascular invasion, lymph node metastasis were two significant clinical attributes of MMPC. MMPC encountered a decreased survival time than PMBC but an increased survival time than IMBC while TNM stage and lymph node metastasis were identified as two independent prognostic elements for DFS of MMPC prognosis.
Conclusions

The data implied that further understanding and classification of MMPC may provide better individualized therapeutic strategies for MMPC treatment.
Gastric Mucin Phenotype Indicated Aggressive Biological Behavior in Early Differentiated Gastric Adenocarcinomas by Endoscopic Treatment
Authors: Song Kai , Yang Qi , Yan Yu , Yu Xiaoyan , Xu Kanlun , Xu Jinghong 
Published: May 2021

Abstract
Background:The distribution of mucin phenotypes and its relationship with clinicopathological features in early differentiated gastric adenocarcinomas among the Chinese cohort is unknow. We aim to investigate the mucin phenotypes and analyze the relationship between mucin phenotypes and clinicopathological features, especially the biological behavior, in early differentiated gastric adenocarcinomas from endoscopic specimens in a Chinese cohort.Methods:Immunohistochemical staining of CD10, MUC2, MUC5AC, MUC6 was performed in 257 patients with early differentiated gastric adenocarcinomas. Tumor location, gross type, tumor size, histological type, depth of invasion, lymphovascular invasion, mucosal background and other clinicopathological parameters were evaluated. Analyzed the relationship between mucin phenotypes and clinicopathological features through chi-square test.ResultsThe incidence of ໿ gastric-, gastrointestinal-, intestinal- and null-phenotype was 21%, 56%, 20% and 3% respectively. The mucin phenotypes were related with histology classification (P༜0.05). The proportion of gastric-phenotype became greater during the transition from differentiated to undifferentiated (P༜0.05). Complete intestinal metaplasia in gastric- and intestinal-phenotype was higher than gastrointestinal-phenotype (P༜0.05). Those mixed with poorly differentiated adenocarcinoma were mainly of gastric-phenotype, which was significantly higher than that of purely differentiated tubular adenocarcinoma (P < 0.05), and the depth of infiltration of mixed type was deeper (P < 0.05). Neither recurrence nor metastasis were detected.ConclusionsThe mucin phenotype of early differentiated gastric adenocarcinoma is of clinical implication, and gastric-phenotype has aggressive biological behavior in early differentiated gastric cancers, especially in those mixed with poorly differentiated adenocarcinoma or papillary adenocarcinoma component.
Randomized Controlled Trial of Neurologic Music Therapy in Parkinson’s Disease: Research Rehabilitation Protocols for Mechanistic and Clinical Investigations
Authors: Buard Isabelle , Lattanzio Lucas , Stewart Rebekah , Thompson Sarah , Sjoberg Kristin , Hookstadt Karen , Morrow Meghan , Holden Samantha K , Sillau Stefan , Thaut Michael , Kluger Benzi 
Published: May 2021

Abstract
BackgroundPresently available medications and surgical treatments for Parkinson’s disease have limited effects on fine motor problems and often leave patients with significant fine motor disability. Standard of care occupational therapy (OT) yields low efficacy, potentially due to a lack of standard protocols. Neurologic Music Therapy (NMT) techniques, especially Rhythmic Auditory Stimulation which relies on interaction between rhythm and movement, have shown to be effective in PD gait rehabilitation possibly through their reliance on neural pathways that are not affected by PD. Therapeutic Instrumental Music Performance (TIMP) is one other NMT technique that holds promise but which mode of action and efficacy has not been investigated in PD yet. MethodsOne hundred PD participants will be randomly assigned to receive 15 sessions of either TIMP with rhythm or TIMP without rhythm, standard of care OT, or to be waitlisted (control) over 5 consecutive weeks. Brain oscillatory responses will be collected using magnetoencephalography during an auditory-motor task to understand the underlying mechanisms. The Grooved Pegboard, the UPDRS III finger tap and the finger-thumb opposition will be assessed to investigate clinical changes related to fine motor function. This project will also serve to confirm or refute our pilot data findings suggesting NMT relies on compensatory brain networks utilized by the PD brain to bypass the dysfunctional basal ganglia. DiscussionThis study aims to use standardized TIMP and OT research protocols for investigating the neuronal pathways utilized by each intervention and possibly study their efficacy with respect to fine motor rehabilitation via a randomized control trial in the PD population.
WITHDRAWN: PBeiD Priority-based Information Dissemination Protocol for V2V Communication
Authors: Tomar Ravi , Sastry Hanumat G , Prateek Manish 
Published: May 2021

Abstract
In Vehicular Ad-hoc Networks (VANET)s, efficient information dissemination plays a vital role in its successful deployment. Broadcasting has proven as one of the better ways for Information Dissemination over vehicular Networks, and cooperative behaviour among vehicles for information exchange is critical. However, the existing broadcast techniques are still suffering from multiple issues such as Broadcast storm problem, network partition problem, and network contention. Motivated from the aforementioned discussion, in this paper, we propose a Priority-based Efficient Information Dissemination Protocol (PBeiD) to improve the broadcast efficiency in VANETs. PBeiD protocol developed with a blend of probability and density-based information dissemination concepts and implemented in the testbed environment using simulation tools consisting of SUMO, OMNET++, and VEINS. The proposed protocol is compared with benchmark protocols, and the simulation is carried out based on different scenarios from sparse to dense. We found that our protocol is performing well in almost all the cases and to provide proper justification that our results are significant and not by chance, we applied statistical t-test on the results obtained.
Comparative Genomics of two Inbred Lines of the Potato Cyst Nematode Globodera rostochiensis reveals disparate Effector Family-specific Diversification Patterns
Authors: Steenbrugge Joris J.M. van , Elsen Sven van den , Holterman Martijn , Sterken Mark G. , Thorpe Peter , Goverse Aska , Smant Geert , Helder Johannes 
Published: May 2021

Abstract
Background: Potato cyst nematodes belong to the most harmful pathogens in potato, and durable management of these soil-borne parasites largely depends on host-plant resistances. These resistances are pathotype specific. The current Globodera rostochiensis pathotype scheme that defines five pathotypes (Ro1 - Ro5) is for fundamental and practical reasons barely useful. As a result, resistant potato varieties are used worldwide in a non-informed manner. Results: We generated two novel reference genomes of G. rostochiensis inbred lines derived from a Ro1 and a Ro5 population. These genome sequences comprise 173 and 189 scaffolds respectively, marking a » 24-fold reduction in fragmentation as compared to the current reference genome. We provide copy number variations for 18 effector families. Four dorsal gland effector families were investigated in more detail. SPRYSECs, known to be implicated in plant defence suppression, constitute by far the most diversified family with 60 and 99 variants in Ro1 and Ro5 distributed over 18 and 26 scaffolds. In contrast, CLEs, effectors involved in feeding site induction, show strong physical clustering. The 10 and 16 variants cluster on respectively 2 and 1 scaffolds. Given that pathotypes are defined by their effectoromes, we pinpoint the disparate nature of the contributing effector families in terms of sequence diversification and loss and gain of variants.Conclusion: Two novel reference genomes allow for nearly complete inventories of effector diversification and physical organisation within and between pathotypes. Combined with insights we provide on effector family-specific diversification patterns, this constitutes a solid basis for an effectorome-based virulence scheme for this notorious pathogen.
Rice agronomic traits and variability induced by mutagenesis
Authors: Soto Alejandro Hernandez , Echeverría-Beirute Fabián , Abdelnour-Esqu Ana , Gatica-Arias Andres , Valdez-Melara Marta 
Published: May 2021

Abstract
Understanding agronomic traits at a genetic level enables the leveraging of this knowledge to produce crops that are more productive and resilient, have better quality and are adjusted for consumer preferences. In the last decade, rice has become a model to validate the function of specific genes, resulting in valuable but scattered information. Here, we aimed to identify particular genes in rice related to traits that can be targeted by different mutation techniques in the breeding of crops. We selected gain of function, misfunction, and specific mutations associated with phenotypes of agronomic interest. The review includes specific trait-related genes involved in domestication, stress, herbicide tolerance, pathogen resistance, grain number, quality, weight, plant structure, nitrogen use, and others. The information presented can be used for rice and crops with similar or homologous genes to breed crops that require improvement to achieve more sustainable production in challenging farming conditions.
The kinetics of tempeh wastewater treatment using Arthrospira platensis
Authors: Riadi Lieke , Askitosari Theresia Desy , Widhi Ragil Puspita Dutaning , Laurensia Melvina , Agustin Yuana Elly , Arifin Yalun 
Published: May 2021

Abstract
The microalga Arthrospira platensis (Spirulina) was used for tempeh wastewater treatment. Microalga growth and the kinetics of chemical oxygen demand (COD) degradation under different light intensities (2,100 and 4,300 lux), tempeh wastewater concentrations (0, 0.5, 1, 1.5% v/v), and sodium nitrate concentrations (0, 0.75, 1, 2, 2.5 g/L) were studied. Improved cell growth in wastewater indicated that mixotrophic growth was preferred. The addition of sodium nitrate up to 2 g/L increased COD removal. The highest COD removal was 92.2%, which was obtained from cultivation with 1% v/v tempeh wastewater, 2 g/L sodium nitrate, 2,100 lux, and the specific growth rate of 0.33 ± 0.01 day−1. The COD removal followed a pseudo-first-order kinetic model with the kinetic constant of 0.3748 day−1 and the nitrate uptake rate of 0.122 g/L-day. The results can be used to design a pilot-scale tempeh wastewater treatment facility using A. platensis for tertiary treatment. Based on the kinetic model, a 20 m3 reactor can treat tempeh wastewater to reduce the COD from 400 to 100 ppm in 4 days and produces approximately 32.8 kg of dried microalgae.