bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rare diseases caused by abnormal calcium sensing and signalling
Authors: Tőke Judit , Czirják Gábor , Enyedi Péter , Tóth Miklós 
Published: February 2021

AbstractThe calcium-sensing receptor (CaSR) provides the major mechanism for the detection of extracellular calcium concentration in several cell types, via the induction of G-protein-coupled signalling. Accordingly, CaSR plays a pivotal role in calcium homeostasis, and the CaSR gene defects are related to diseases characterized by serum calcium level changes. Activating mutations of the CaSR gene cause enhanced sensitivity to extracellular calcium concentration resulting in autosomal dominant hypocalcemia or Bartter-syndrome type V. Inactivating CaSR gene mutations lead to resistance to extracellular calcium. In these cases, familial hypocalciuric hypercalcaemia (FHH1) or neonatal severe hyperparathyroidism (NSHPT) can develop. FHH2 and FHH3 are associated with mutations of genes of partner proteins of calcium signal transduction. The common polymorphisms of the CaSR gene have been reported not to affect the calcium homeostasis itself; however, they may be associated with the increased risk of malignancies.
CPMS–improving patient care in Europe via virtual case discussions
Authors: Mönig Isabel , Steenvoorden Danielle , de Graaf Johan P. , Ahmed S. Faisal , Taruscio Domenica , Beun Johan G. , Johannsen Trine H. , Juul Anders , Hiort Olaf , Pereira Alberto M. 
Published: February 2021

Abstract
Purpose
The core task of European Reference Networks (ERNs) is to reduce health care inequalities throughout Europe for all patients with rare and complex conditions. A secure web-based application for virtual consultations, the Clinical Patient Management System (CPMS), was developed by the EU to provide expert specialized care for all these patients. This review analyses the opportunities and difficulties that the implementation of this virtual network implies for physicians as well as for the patients.

Methods
European Reference Network on Rare Endocrine Conditions (Endo-ERN) installed an Operational Helpdesk (OH) to support their members in using CPMS. The OH initiated several actions to facilitate and increase the usage of CPMS. Satisfaction with the system and reasons for low participation rates in virtual case discussions were analyzed by different surveys.

Results
The number of CPMS users increased constantly, but the active usage of the system remains insufficient. Main reasons were technical difficulties, lack of time and insufficient awareness about CPMS in experts and patients throughout Europe. Still, outcomes of the virtual discussions are considered useful by involved experts and the discussions have provided topics for educational webinars and research.

Conclusions
CPMS is a secure system with many advantages compared to previous ways of consulting experts but also difficulties that need to be overcome with future strategies. By facilitating its use and increasing awareness among all relevant European experts and patients, CPMS can help to make the existing expertise available for all patients with rare (endocrine) conditions throughout Europe as it was intended.
Informational architecture across non-living and living collectives
Authors: Kim Hyunju , Valentini Gabriele , Hanson Jake , Walker Sara Imari 
Published: February 2021

AbstractCollective behavior is widely regarded as a hallmark property of living and intelligent systems. Yet, many examples are known of simple physical systems that are not alive, which nonetheless display collective behavior too, prompting simple physical models to often be adopted to explain living collective behaviors. To understand collective behavior as it occurs in living examples, it is important to determine whether or not there exist fundamental differences in how non-living and living systems act collectively, as well as the limits of the intuition that can be built from simpler, physical examples in explaining biological phenomenon. Here, we propose a framework for comparing non-living and living collectives as a continuum based on their information architecture: that is, how information is stored and processed across different degrees of freedom. We review diverse examples of collective phenomena, characterized from an information-theoretic perspective, and offer views on future directions for quantifying living collective behaviors based on their informational structure.
Are women the more empathetic gender? The effects of gender role expectations
Authors: Löffler Charlotte S. , Greitemeyer Tobias 
Published: February 2021

AbstractThe present research aimed to extend the state of knowledge regarding the relationship between self-perceived empathy and traditional gender roles and placed particular focus on the contextual conditions under which gender differences in empathy are present, can be created, or eliminated. Across two studies, women rated themselves higher in empathy than men in all experimental conditions, whereas an objective female superiority in emotion recognition was only evident in one condition. In Study 1 (n = 736), using the term ‘social-analytic capacity’ instead of ‘empathic capacity’ increased gender differences in self-reported empathy and resulted in women performing better in the Eyes-test than men. In a neutral task (verbal intelligence), gender differences (in this case, a male superiority), were only found when participants believed that this task had an association with empathy. In Study 2 (n = 701), gender differences in self-reported empathic capacity, but not in performance in emotion recognition, increased when motivation for empathy was raised. Further, gender-role orientation mediated the association between gender and self-reported empathic capacity, whereas it did not account for the association between gender and emotion recognition. Overall, the present studies provide strong support for the idea that empathy is influenced by contextual factors and can be systematically biased by gender roles and stereotypical beliefs.
Personal values as early predictors of emotional and cognitive empathy among medical students
Authors: Ardenghi Stefano , Rampoldi Giulia , Bani Marco , Strepparava Maria Grazia 
Published: February 2021

AbstractThe present study contributes to the literature by examining the association between personal values (PVs), assessed with the Schwartz’s Portrait Values Questionnaire, and empathy, assessed with the Davis’ Interpersonal Reactivity Index, in a sample of first-year medical students. We also examined medical students’ PVs profile and gender differences in terms of PVs. All participants (N = 398) were Italian, young (average age = 19.62 years, SD = 1.22), and unmarried; none had children. Zero-order correlations and hierarchical multiple regression models were performed to verify the association between PVs and empathy; in contrast, t-tests were run to explore gender differences in scoring on PVs. Benevolence and Universalism correlated positively with both the emotional and cognitive dimensions of empathy, whereas Power, Achievement, Hedonism, and Security were negatively associated with empathy. The three most important PVs in the whole sample were Benevolence, Self-Direction, and Universalism. Male medical students outscored their female counterparts on Power, Achievement, and Hedonism, whereas female students outscored the males on Benevolence, Universalism, Conformity, and Tradition. Our findings highlight the importance of fostering self-transcending PVs and discouraging self-enhancing PVs in medical students during the early years of medical school, as a means of supporting other-oriented responses such as empathy in future doctors.
Microglial Exosome miR-7239-3p Promotes Glioma Progression by Regulating Circadian Genes
Authors: Li Xuepei , Guan Junwen , Jiang Zhou , Cheng Shuting , Hou Wang , Yao Junjie , Wang Zhengrong 
Published: February 2021

AbstractGlioma-associated microglial cells, a key component of the tumor microenvironment, play an important role in glioma progression. In this study, the mouse glioma cell line GL261 and the mouse microglia cell line BV2 were chosen. First, circadian gene expression in glioma cells co-cultured with either M1 or M2 microglia was assessed and the exosomes of M2-polarized and unpolarized BV-2 microglia were extracted. Subsequently, we labeled the exosomes with PKH67 and treated GL261 cells with them to investigate the exosome distribution. GL261 cell phenotypes and related protein expression were used to explore the role of M2 microglial exosomes in gliomas. Then a specific miR-7239-3p inhibitor was added to verify miR-7239-3p functions. Finally, the mouse subcutaneous tumorigenic model was used to verify the tumorigenic effect of M2 microglial exosomes in vivo. Our results showed that in gliomas co-cultured with M2 microglia, the expression of the BMAL1 protein was decreased (P < 0.01), while the expression of the CLOCK protein was increased (P < 0.05); opposite results were obtained in gliomas co-cultured with M1 microglia. After treatment with M2 microglial exosomes, the apoptosis of GL261 cells decreased (P < 0.001), while the viability, proliferation, and migration of GL261 cells increased. Increased expression of N-cadherin and Vimentin, and decreased E-cadherin expression occurred upon treatment with M2 microglial exosomes. Addition of an miR-7239-3p inhibitor to M2 microglial exosomes reversed these results. In summary, we found that miR-7239-3p in the glioma microenvironment is recruited to glioma cells by exosomes and inhibits Bmal1 expression. M2 microglial exosomes promote the proliferation and migration of gliomas by regulating tumor-related protein expression and reducing apoptosis.
Rolle der Dokumentations-IT in der Rheumatologie
Authors: Pallua Johannes , Schirmer Michael 
Published: February 2021

ZusammenfassungDie Entwicklung der „digitalen Gesundheit“ hat zahlreiche Implikationen für die moderne Medizin, mit vielen Möglichkeiten und Herausforderungen auch für die Rheumatologie. Fundamentale Voraussetzung dafür ist die IT-Dokumentation. Die Verpflichtung zur Dokumentation zusammen mit einer regelmäßigen Qualitätsevaluierung ergibt sich für den Arzt aufgrund der gesetzlichen Vorgaben. Die Vielzahl an klinischen Anforderungen muss durch eine gute Dokumentations-IT auch technisch bewältigt werden. Die Qualität der Dokumentation ist für alle weiteren Datenanwendungen entscheidend. Die Vielfalt der rheumatologischen Diagnosen, zusammen mit den oft zahlreich involvierten anderen Fachdisziplinen, erfordert eine flexible und situationsspezifische Dokumentations-IT, welche die fachlichen Entwicklungen am neuesten Stand abbilden kann. In Österreich ist die elektronische Gesundheitsakte als Grundlage der Dokumentations-IT zwar zentral verwaltet, ist aber durch dezentrale Lösungen zu ergänzen. Eine gute Dokumentations-IT erfüllt in Zukunft sicher mehr als eine reine Dokumentation von klinischen Daten und Leistungen. Dadurch kann sich eine gute Dokumentations-IT zu einem wertvollen Werkzeug des Arztes entwickeln, das diesem hilft, umfassend in Hinblick auf alle vorliegenden Befunde die besten Entscheidungen zu treffen.
A Review of Treatment of Bilateral Vocal Fold Movement Impairment
Authors: Dunya Gabriel , Orb Quinn T. , Smith Marshall E. , Marie Jean-Paul 
Published: February 2021

Abstract
Purpose of Review
Bilateral vocal fold immobility is a challenging life-threatening problem involving multiple treatment options and nuanced clinical decision making. We aim to provide relevant background on the etiology, diagnosis, and management of bilateral vocal fold movement impairment (BVFMI).

Recent Findings
Over the last 20 years, the management of bilateral vocal fold immobility has advanced significantly with the addition of multiple endoscopic approaches as well as procedures with the goal of returning dynamic function to the larynx, among them: selective reinnervation. Chemodenervation has also demonstrated promising results as a temporizing procedure in appropriately selected patients with BVFMI.

Summary
Tracheostomy remains the mainstay of emergent treatment for airway obstruction secondary to bilateral vocal fold immobility. However, recent advances in endoscopic approaches allow for avoidance of tracheostomy in many patients. Developments in dynamic procedures with the aim of restoring laryngeal function allow for adequate airway management while maintaining voice quality and limiting aspiration risk.
Air Quality Over Major Cities of Saudi Arabia During Hajj Periods of 2019 and 2020
Authors: Farahat Ashraf , Chauhan Akshansha , Al Otaibi Mohammed , Singh Ramesh P. 
Published: February 2021

AbstractMecca and Madinah are two holy cities where millions of people in general, visit throughout the years, during Hajj (Muslim's pilgrimage) time number of people visit these holy cities from different parts of the world is very high. However, the Government of Saudi Arabia only allowed 1000 pilgrims during the 2020 Hajj especially when the world is suffering from COVID-19. In the present paper, a detailed analysis of air quality parameters available from ground measurements have been carried over major cities of Saudi Arabia, Mecca, Madinah, and Jeddah from June to September 2019 and 2020. At Mecca and Jeddah, PM10, NO2 and CO concentrations are found to be higher in comparison to stations located close to Madinah. In addition, meteorological parameters, temperature, relative humidity, and wind directions are also analysed to understand changes associated with the meteorological parameters. Our detailed analysis shows a reduction in concentrations of pollutants during the 2020 Hajj, when the lockdown was observed in Saudi Arabia due to COVID-19. During June–August 2020 lockdown period, no major changes in PM10 concentration was observed since there were frequent dust events were observed. In contrast, our results show 44% reduction in NO2 (8.77 ppbv in 2019 to 4.97 ppbv in 2020) and 16% reduction in CO (0.85 ppm in 2019 to 0.72 ppm in 2020) concentrations during COVID-19 restrictions. The concentrations of NO2 and CO do not cause any issue for the air quality as NO2 and CO Indices lie within the green band (Air quality index 0–50). In Mecca, Madinah and Jeddah, the air quality is generally affected during Hajj, but during 2020, the concentration of particulate matter was influenced by local weather conditions.
Entrepreneurship education at universities: challenges and future perspectives on online game implementation
Authors: Takemoto Takuji , Oe Hiroko 
Published: February 2021

AbstractThe study explored the challenges and potential of online gamification to develop actionable recommendations for entrepreneurship pedagogy in the phase of ‘new normal’. This study applied an experimental game which the authors developed, and a mixed method was applied to the data sets collected from the students: an open-ended survey of 91 students and in-depth interviews with 23 students. It has been found that the students perceiving activities with gamifications are good learning stimuli in entrepreneurial classrooms as a first step; then, they found it effective to learn and deepen their understanding of theories and models as a second step after the gaming activities, which is a reverse approach from a traditional business education approach. Gamification enables students to think critically on game scenarios via participation in gamifications, which can be strengthened and embedded in their mind by theoretical learning which follows the gaming activities. The findings of the study provide a practical guidance for entrepreneurship pedagogists with ‘activities first’ which will be followed by theoretical learning.
Anchored k-medoids: a novel adaptation of k-medoids further refined to measure long-term instability in the exposure to crime
Authors: Adepeju Monsuru , Langton Samuel , Bannister Jon 
Published: February 2021

AbstractLongitudinal clustering techniques are widely deployed in computational social science to delineate groupings of subjects characterized by meaningful developmental trends. In criminology, such methods have been utilized to examine the extent to which micro places (such as streets) experience macro-level police-recorded crime trends in unison. This has largely been driven by a theoretical interest in the longitudinal stability of crime concentrations, a topic that has become particularly pertinent amidst a widespread decline in recorded crime. Recent studies have tended to rely on a generic implementation k-means to unpick this stability, with little consideration for its theoretical suitability. This study makes two methodological contributions. First, it demonstrates the application of k-medoids to study longitudinal crime concentrations, and second, it develops a novel ‘anchored k-medoids’ (ak-medoids), a bespoke clustering method specifically designed to meet the theoretical requirements of micro-place investigations into long-term stability. Using both simulated data and 15-years of police-recorded crime data from Birmingham, England, we compare the performances of k-medoids against ak-medoids. We find that both methods highlight instability in the exposure to crime over time, but the consistency and contribution of cluster solutions determined by ak-medoids provide insight overlooked by k-medoids, which is sensitive to short-term fluctuations and subject starting points. This has important implications for the theories said to explain longitudinal crime concentrations, and the law enforcement agencies seeking to offer an effective and equitable service to the public.
Supercritical fluid extraction of Bhut Jolokia oleoresin and its quality analysis
Authors: Deka Darshana , Swami Hulle Nishant Rachayya 
Published: February 2021

AbstractThe present study focuses on the standardization of the supercritical fluid extraction of the Bhut Jolokia (Capsicum chinense) which is abundantly grown in the north eastern region of India. The effect of process parameters pressure (75–225 bar), temperature (40–60 °C) and time (30–90 min) of oleoresin extraction process was studied. The standardized condition to obtain maximum extraction of Bhut Jolokia oleoresin was found to be 207 bar, 60 °C and 73 min. The oleoresin extract was analyzed for its antimicrobial, antioxidant and total phenolics content. Four strains of bacteria namely Escherichia coli (ATCC -11,229), Bacillus subtilis (ATCC- 11,774), Salmonella typhimurium (ATCC- 14,028) and Staphylococcus aureus (12,600) were used for the antimicrobial assay. It was observed that the highest inhibition was seen against E. coli, moderate inhibition was seen against S.aureus and S. typhi and partial/no zone of inhibition was observed against B. subtilis. The extract of Bhut Jolokia oleoresin showed radical scavenging activity of 58.6 ± 3.86% and total phenolics content of 4250 ± 2.26 mg GAE/100 g sample indicating Bhut Jolokia oleoresin as a good antioxidant and is also a good source of phenolic compounds.
Geochemical variability in the soils of Bangladesh as affected by sources of irrigation water and inundation land types
Authors: Chowdhury M. Tanvir A. , Meharg Andrew A. , Price Adam H. , Norton Gareth J. 
Published: February 2021

AbstractPaddy soils in Bangladesh experience extensive irrigation with groundwater and surface water, both having variable geochemical constituents. The soils also have topological variations across the landscape. To understand the geochemical variability in the soils as affected by the different sources of irrigation water and the topographical variability, cultivation zones of paddy soils irrigated with both groundwater (n = 904) and surface water (n = 281) across Bangladesh were sampled and analyzed for a suit of seventeen geochemical elements. This study also assessed the extent and distribution of arsenic and the other geochemical elements in the paddy soils (n = 1209) as well as in a set of neighboring non-paddy soils (n = 235) within the different inundation land types (highland, medium highland-1, medium highland-2, medium lowland, lowland and very lowland) of Bangladesh. The mean concentrations of aluminum (26,000 mg/kg), cobalt (13 mg/kg), copper (32 mg/kg), iron (28,250 mg/kg), lead (18 mg/kg), magnesium (8050 mg/kg), molybdenum (1.02 mg/kg), nickel (41 mg/kg), potassium (4870 mg/kg), sodium (750 mg/kg) and zinc (70 mg/kg) in the surface water-irrigated paddy soils were found to be significantly (0.001 ≥ p ≤ 0.05) higher compared to the concentrations in the soils irrigated with groundwater (23,400; 12; 28; 25,650; 17; 7000; 0.96; 36; 4350; 600; and 62 mg/kg, respectively). Therefore, surface water used for paddy irrigation could increase the inputs of a number of toxic elements in the paddy soils having potential risk of crop contamination. Arsenic in the paddy and non-paddy soils varied significantly (F = 24.74, p < 0.001 and F = 3.42, p < 0.01, respectively) within the inundation land types, the very lowland (9.95 and 6.72 mg/kg, respectively) and lowland (8.33 and 5.20 mg/kg, respectively) having the highest mean arsenic concentrations and the medium highland-1 (5.27 and 5.17 mg/kg, respectively) having the lowest. The concentrations of the other geochemical elements analyzed were also observed to be higher, in general, in the soils of very lowland and lowland. Since the low-level lands are predominantly used for paddy cultivation, higher concentrations of various toxic elements, particularly arsenic, in such soils pose an increased risk of rice toxicity in Bangladesh. The results of this study present an inimitable geochemical database for the surface soils across Bangladesh which can be used in any future studies on the geomorphologically variable agricultural and non-agricultural Bangladeshi soils, providing a basis for environmental pollution assessment and sustainable mitigation approaches.
Which Happens Earlier, Lower Urinary Tract Symptoms or Erectile Dysfunction?
Authors: Matsuda Yohei , Kobayashi Ko , Fukuta Fumimasa , Takayanagi Akio , Hashimoto Kohei , Tanaka Toshiaki , Masumori Naoya 
Published: February 2021

Abstract

Introduction
Although an association between erectile dysfunction (ED) and lower urinary tract symptoms (LUTS) has been suggested, it was not clarified whether LUTS developed before ED or vice versa.

Aim
To clarify whether LUTS develop before ED or vice versa and which symptoms predicted the onset of the other condition in a longitudinal community-based study.

Methods
We conducted a longitudinal community-based study on LUTS and ED in aged Japanese men. A follow-up study was conducted to determine their longitudinal changes of LUTS and ED after 15 years. Erectile function was evaluated using a validated questionnaire. LUTS were evaluated based on the International Prostate Symptom Score, quality of life index, and prostate volume.

Main outcome measure
We evaluated the baseline symptoms among the participants who had LUTS and ED in the follow-up survey and what prior symptoms could predict the onset of the other condition using the data from a long-term longitudinal survey.

Results
A total of 108 men were enrolled in this study. Of the 47 men having both LUTS and ED in the follow-up study, men having only LUTS (n = 16) were more frequent than those having only ED (n = 6) in the initial study. Likewise, of the 38 men having both nocturia and ED at the time of the follow-up study, those having only nocturia (n = 12) were more frequent than those having only ED (n = 5) in the initial study. In multivariable analysis, age 60 years or older (odds ratio: 7.10, 95% CI: 2.09-24.13) and nocturia (odds ratio: 15.83, 95% CI: 3.05-82.15) were independent predictors for the onset of ED.

Conclusion
There were more men with prior onset of LUTS, especially nocturia, than men with prior onset of ED among those with both ED and LUTS in this long-term longitudinal study. Nocturia may be a predictor of subsequent ED.