bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soufeng Sanjie Formula Alleviates Collagen-induced Arthritis in Mice by Inhibiting Th17 Cell Differentiation
Authors: Hua Di , Yang Jie , Meng Qinghai , Ling Yuanyuan , Wei Qin , Wang Zhigang , Wei Qingyun , Chen Jiao , Ye Juan , Han Xuan , Su Kelei , Kong Weikang , Xu Chao , Cao Peng , Hu Chunping 
Published: March 2021

Abstract
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease. Soufeng sanjie formula (SF), which is composed of scolopendra (dried body of Scolopendra subspinipes mutilans L. Koch), scorpion (dried body of Buthus martensii Karsch), astragali radix (dried root of Astragalus membranaceus (Fisch.) Bge), and black soybean seed coats (seed coats of Glycine max (L.) Merr), and is a traditional Chinese prescription for treating RA. However, the mechanism of SF in treating RA remain unclear. This study was aim to investigate the anti-arthritic effects of SF in a collagen-induced arthritis (CIA) mouse model and explore the mechanism by which SF alleviates arthritis in CIA mice.Methods: For in vivo studies, female DBA/1J mice were used to establish the CIA model, and either SF (183 or 550 mg/kg/d) or methotrexate (MTX, 920 mg/kg, twice/week) was orally administered to the mice from the day of arthritis onset. After administration for 30 d, degree of ankle joint inflammatory infiltration and serum levels of IgG and inflammatory cytokines were determined. The balance of Th17/Treg cells in the spleen and lymph nodes was analyzed using flow cytometry. Furthermore, the effect of SF on Th17 cells differentiation in vitro was investigated in Th17 polarization.Results: SF decreased the arthritis score, ameliorated paw swelling, and reduced synovial hyperplasia in the joints of CIA mice. In addition, SF decreased the levels of bovine collagen-specific IgG and inflammatory cytokines (TNF-α, IL-6, and IL-17A) and increased the level of IL-10 in the sera of CIA mice. Moreover, SF treatment rebalanced the Th17/Treg ratio in the spleen and lymph nodes of CIA mice. In vitro, SF treatment reduced Th17 cell generation and IL-17A production and inhibited the expression of RORγt, IRF4, and Il-17A under Th17 polarization conditions.Conclusions: Our results suggest that SF exhibits anti-arthritic effects and restores Th17/Treg homeostasis in CIA mice by inhibiting Th17 cell differentiation.
Polydiacetylene Vesicles Acting as Colorimetric Sensor for the Detection of Plantaricin LD1 Purified From Lactobacillus Plantarum LD1
Authors: Yadav Manoj Kumar , Tiwari Santosh Kumar 
Published: March 2021

Abstract
The interaction of antimicrobial peptides with membrane lipids plays a major role in numerous physiological processes. Bacteriocins are antimicrobial peptides known to kill target cells by pore formation and membrane disruption. In this study, polydiacetylene (PDA) vesicles were applied as artificial membrane for detection of plantaricin LD1 purified from Lactobacillus plantarum LD1. Plantaricin LD1 (200 µg/ml) was able to change the color of PDA vesicles from blue to red with colorimetric response CR % 30.26 ± 0.59. Nisin (200 µg/ml), used as control, also changed the color of the vesicles with CR % 50.56 ± 0.98 validating the membrane-acting nature of these bacteriocins. The PDA vesicles treated with nisin and plantaricin LD1 showed increased infrared absorbance at 1411.46 cm-1 and 1000-1150 cm-1 indicated the interaction of bacteriocins with phospholipids and fatty acids, respectively. Further, microscopic examination also suggested the disruption of bacteriocin-treated vesicles indicating the interaction of bacteriocins. These findings suggest that the PDA vesicles may be used as bio-mimetic sensor for the detection of bacteriocins produced by several probiotics in food and therapeutic applications.
Dominating the Structural, Microstructural and Magnetic Features of Li+ Substituted Strontium Hexaferrite (Sr1-xLi2xFe12O19)
Authors: Hessien Mahmoud , El-Bagoury Nader , Mahmoud M. H. H. , Alsawat M. , ALanazi Abdullah , Rashad M. M. 
Published: March 2021

Abstract
Lithium ion substituted hexagonal strontium ferrite (Sr1-xLi2xFe12O19, where x= 0.1, 0.2, and 0.3) powders have been felicitously fabricated using tartrate precursor scheme. The impact of the Li+ content, as well as the annealing temperature on the phase evolution, microstructure and magnetic performance, was commanded by X-ray diffraction profile (XRD), scanning electron microscopy (SEM), and vibrating sample magnetometer (VSM). Single phase hexagonal ferrite was consummated at a Li+ ratio of 0.2 and different annealing temperatures, from 1000 to 1200oC for 2h. An impurity α-Fe2O3 phase was noted at a high Li+ concentration of 0.4 and 0.6 at all temperatures. The crystallo-aspects characteristics were altered with Li+ content and annealing temperature. The microstructure of pure hexagonal ferrite sample visualized platelet like structure. A fine spherical shape displayed with platelet shape by enhancing the Li+ content up to 0.4 and 0.6. EDX analysis emphasized Fe, Sr, O, and Li atoms spread between the plate and spherical shapes. Good saturation magnetization (Ms=60.88 emu/g) was realized for Li+ content of 0.2 as the results of increasing the thickness of the nanoplatelet structure.
Ultralow loss topological metamaterial in the visible spectrum
Authors: Zhao Jing , Chen Huan , Song Kun , Xiang Liqin , Zhao Qian , Shang Chaohong , Wang Xiaonong , Shen Zhijie , Wu Xianfeng , Hu Yajie , zhao xiaopeng 
Published: March 2021

Abstract
Optical metamaterials give birth to the control and regulation of light. However, because of strong energy dissipation and fabrication difficulty in metals, low-loss isotropic three dimentional negative index metamaterials (NIMs) in the visible spectrum has long been regarded as an extremely challenging. Here we report an ultralow loss isotropic topological metamaterials for visible light. The ball-thorn-shaped meta-clusters with symmetrical structure consisting of dielectric and topological silver layer was proposed, the surface plasma resonance is formed inside a cluster unit with a height of two atomic layers, resulting in an optimized silver coating thickness about 1 nm. We invented a unique technique for preparing ultralow loss isotropic clusters and three-dimensional large area samples. The negative refractive index and the inverse Doppler effect of green and red light is measured by the prism method for the first time. This molecular crystals NIMs break through noble metal important energy losses and manufacturing difficulties.
Effect of Small Molecular Compounds on Properties of Fibroblast in Chuan Snub-nosed Monkey
Authors: wang juanjuan , liu xin , yang jing , guo hanxing , li jingjing , huo lihui , zhao haitao , wang xiaowei , yan xingrong , li baoguo 
Published: March 2021

Abstract
Small molecular compounds could improve the induction efficiency of induced pluripotent stem cells (iPS). To investigate their effects on the efficiency of interspecies nuclear transfers, fibroblasts from the Chuan snub-nosed monkey were treated with small molecular compounds and used as donor cells to be injected into the enucleated oocytes of a goat. The gene expression profiles in the cell-constructed embryos, with and without the small molecular compound treatments, were determined by qPCR. Results showed that the cell morphology showed obvious changes, while the gene expression profiles of the fibroblasts were altered by the treatment. The pluripotent genes (Oct4, sox2, and nanog) were significantly increased on treatment with the small molecular compounds. Results demonstrated that these small molecular compounds could alter the properties of the donor cells, to promote the expression levels of the pluripotent genes for the Chuan golden-goat interspecies embryo, which would provide references for conservation of Chuan snub-nosed monkey.
The Baseline Characteristics of Patients&nbsp;with COVID-19 and Impact of Various Risk Factors on the Outcome of Patients Admitted in Tertiary Care Hospital.
Authors: Shaikh Samiullah , Deedar Faiza , Devrajani Tara Chand 
Published: March 2021

Abstract
Background: The outbreak caused by novel coronavirus was first reported in Wuhan, China in December 2019 when a new pathogen was discovered from the bronchoalveolar lavage fluid of the patient suffering from pneumonia. Globally over 81.47 million confirmed cases and 1.79 million deaths has been reported. The clinical spectrum ranges from mild influenza-like illness to severe life threatening respiratory disease requiring ventilatory support to death. The purpose of this study was to evaluate the baseline carecteristic of the patients and impact of various risk factors on the outcome of patients admitted in tertiary care hospital. MATERIALS AND METHODSStudy Area and Period: This study was conducted at Liaquat University Hospital from April 2020 to September 2020. Study Design: Cross-Sectional Descriptive.Inclusion Criteria: The patients were included on the basis of diagnosis of COVID-19 confirmed on reverse transcription Polymerase Chain reaction (RT-PCR) test. Categorical variables such as age, sex, Gram-Covid risk category ,diabetes mellitus, hypertension, renal, pulmonary disease, obesity, outcome of patient were presented as frequency and percentage RESULTS: This study included 235 consecutive patients admitted at Liaquat University Hospital from April 2020 to September 2020. There were 187(79.6%) male and 48(20.4 %) female. The age < 65 years were present in 80 ( 34%) and ≥ 65 155(66%) patients. The most prevalent symptoms were fever ,breathlessness, cough, anorexia, fatigue in all 235(100% ) patients. Severity of illness according to Gram-Covid Score 18 (7.7%) patients were in low-risk group, 93(39.6%) in moderate risk group and 124 (52.8%) in High risk group.There were no risk factors in 121(51.5%) , one risk factor in 49(20.9%), two risk factors in32(13.6%) , three risk factors in 18( 7.7%) and four risk factors in 15(6.4%) patients.The outcome of patients showed 57(24.3%) deaths, 107 (45.5%) patients had prolong stay and 71(30.2%) patients recovered. A strong relationship was observed between the outcome of patient with age, number of risk factors, severity of illness according to gram - Covid score whereas insignificant relationship with the sex of patients.Conclusion: COVID-19 patients clinically present with fever, cough and breathlessness. Majority of patients present with moderate to high severity. Patient with advanced age, high risk on gram-Covid score with multiple comorbidities are particularly susceptible to adverse outcome.
WITHDRAWN: Diagnostic and Therapeutic Challenges in The Management of Acute Massive Overt Bleeding of Jejunal Gastrointestinal Stromal Tumours: Case Series
Authors: NAGARAJ SATISH SUBBIAH , Deivasigamani Sriram , Aruni Amresh , Kumar Hemanth , Sachan Anurag , Samanta Jayanta , Bal Amanjit 
Published: March 2021

Abstract
Introduction:
Jejunal Gastro-Intestinal Stromal Tumours (GIST) are rare mesenchymal tumours. Acute massive overt bleeding from jejunal GIST are very rare and poses both diagnostic and therapeutic challenges in emergent conditions.
Methods
A case series with retrospective analysis of prospectively maintained database of patients presenting with acute massive overt bleeding secondary to histologically proven jejunal GIST was done. Clinical characteristics, endoscopic and imaging diagnostic features, histological findings, surgical procedures and outcomes in these patients were studied.
Results
Three patients were included in this case series. Mean age of presentation was 49.0 years with two male and one female patient. All three patients presented with melena and hemodynamic instability, resuscitated with adequate blood transfusions. Routine endoscopic assessment were inconclusive. Multiphasic Computed Tomographic Angiography (CTA) revealed hypodense hypervascular mass in jejunum in all three patients. One patient was unresponsive to blood transfusion and underwent emergency exploratory laparotomy. One patient underwent laparoscopic resection and reconstruction. Mean length of hospital stay was 5.3 days. Histopathological examination confirmed Jejunal GIST in all three patients with microscopically negative resection margins. Two patients were disease free till 18 months follow up and the one patient lost to follow up after 1 year.
Conclusion
Multiphasic CTA is a single step diagnostic tool for localisation of bleed and assessment of tumour characteristics in emergent conditions. Surgical resection is the mainstay of treatment for both control of bleed and to provide oncologically clear resection margins.
Diagnostic Accuracy of the Screening Instruments Used to Identify Postpartum Depression in the Primary Care Setting: Protocol for a Systematic Review and Meta-Analysis
Authors: Andriani Russy Novita , Makkiyyah Siti Solichatul , Hertika Amanda Safira Dea , Istiono Wahyudi , Hakimi Mohammad 
Published: March 2021

Abstract
Background
Postpartum depression (PPD) is a prevalent complication of pregnancy, this condition affects maternal and child well-being and functioning. Results from a meta-analysis showed an incidence of 13% PPD cases in the first 12 weeks after labor. Primary care is the first gate and continuing point of care for patients. Despite the controversy of screening and early identification in primary care settings, many PPD cases remain undetected. Given the uncertainty about this issue, screening instruments must be effective in identifying the cases. This systematic review and meta-analyses aim to identify the most suitable postpartum depression screening instrument for use in primary care.
Methods
PubMed, ScienceDirect, and ProQuest databases were used to search using relevant keywords or MeSH, with limitation of publication from January 1st, 2010 through December 31st, 2020. We will include screening studies on postpartum women using validated screening tools followed by validated structured or semi-structured interview for Diagnostic and Statistical Manual of Mental Disorders (DSM) as the reference standard in the primary care setting. Study designs included in the review are cross-sectional and randomized controlled trial without no screening arm on the diagnostic study. We will use a liberal accelerated method on the title and abstract review stage, then perform full-text article reviews on selected studies. Methodological quality will be assessed independently by two authors according to QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies-2). Extraction of the study data will be undertaken by one reviewer and checked by a second reviewer. Disagreements will be resolved by consensus and including a third investigator as necessary. The test characteristics will be extracted into 2x2 tables for all included studies. Study-specific estimates of sensitivity and specificity with 95% confidence intervals will be displayed in forest plots.
Discussion
The proposed systematic review and meta-analyses will allow us to obtain the most suitable postpartum depression screening instrument for use in primary care.
Systematic review registration:
PROSPERO CRD42020216067
Bedding Effect of Carbonaceous Slate Failure Behavior at Direct Shear Tests
Authors: Wang Han , Ren Fuqiang , Chang Yuan 
Published: March 2021

Abstract
In this study, the carbonaceous slate of Muzhailing tunnel was selected to explore the deformation and failure mechanism of tunnel layered slate under direct shear conditions. Five sets of direct shear tests were carried out on slate with different bedding dip angles (β: 0°, 30°, 45°, 60°, and 90°). The strength parameters, failure mode, fracture topography, dissipated energy, and acoustic emission (AE) characteristics were analyzed in detail to study the bedding effect of slate failure. The results show that the cohesion and internal friction angle of slate increases and decreases linearly with β from 0° to 90°, respectively, and the shear strength of bedding plane is lower than that of matrix. Additionally, the relative roughness (δ) of gray-scale image was defined, and the linear relationship between δ and root-mean-square height (Sq) was established to improve the efficiency of evolution of fracture topography. Furthermore, the shear energy consumption per unit area (us) was calculated, less sensitive to the variation of β. Moreover, a larger AE count and energy release rate were observed when the shear stress decreased, and a relative quiet period was observed before the peak loading. Additionally, both the count and energy release rate linearly decreased with the increase in β, and the proportion of tensile microcracks for vertical bedding is slightly higher than that for horizontal bedding. The main frequency of vertical bedding slate is smaller than that of horizontal bedding, i.e., the size of microcracks of slate with β of 90° is relatively larger. Generally, the frequency bandwidth of slate became narrower when β was varied from 30° to 60°, and the main frequency increased, indicating that the degree of shear failure of matrix is weakened. In contrast, the degree of cracking along the bedding plane increased.
Cardiorespiratory Monitoring of Red Blood Cell Transfusion in Preterm Infants
Authors: Poppe Jarinda A. , Essen Tanja van , Weteringen Willem van , Willemsen Sten P. , Reiss Irwin K.M. , Simons Sinno H.P. , Jonge Rogier C.J. de 
Published: March 2021

Abstract
Clinical improvement after red blood cell (RBC) transfusions in preterm infants remains debated. This study aims to investigate the effect of RBC transfusion on the occurrence of desaturations and hypoxia and other cardiorespiratory outcomes in preterm infants. In this longitudinal observational study, prospectively stored cardiorespiratory parameters of preterm infants who received at least one RBC transfusion between July 2016 and June 2017 were analyzed. Sixty infants with 112 RBC transfusions, median GA of 26.7 weeks, were included. The number of desaturations and area <80% SpO2 limit, as a measure of the hypoxic burden, were calculated in 24h before and after RBC transfusion. A mixed effects model was used to account for repeated measurements. Overall, mean (SE) number of desaturations per hour decreased from 3.28(0.55) to 2.25(0.38; p<0.001) and area <80% SpO2 limit decreased from 0.14(0.04) to 0.08(0.02) %/sec (p=0.02). These outcomes were stratified for number of desaturations in 24h prior to RBC transfusion. The largest effect was observed in the group with the highest mean number of desaturations (≥6) prior to RBC transfusion, with a decrease from 7.50(0.66) to 4.26(0.38) (p<0.001) in number of desaturations and 0.46(0.13) to 0.20(0.06) in area <80% SpO2. Perfusion index increased significantly after RBC transfusion (p<0.001). No other significant effects of RBC transfusion on cardiorespiratory data were observed.Conclusions: RBC transfusions in preterm newborns decrease the incidence of desaturations and the hypoxic burden. The higher the number of desaturations prior to the RBC transfusion, the larger the effect observed.
Medication incidents and pharmacist interventions in relation to directly acting oral anticoagulants in hospital settings: evaluation using Reason’s Accident Causation Theory
Authors: Haque Hazera , Alrowily Abdulrhman , Jalal Zahraa , Tailor Bijal , Efue Vicky , Sarwar Asif , Paudyal Vibhu 
Published: March 2021

Abstract
BackgroundDirect oral anticoagulants (DOACs) have revolutionised anticoagulant pharmacotherapy. However DOACs medication incidents are known to be common.ObjectiveTo assess medication incidents associated with DOACs using an error theory and to analyse pharmacists’ contributions in minimising medication incidents in secondary care settings.SettingA large University tertiary academic hospital in the West Midlands of England.MethodsMedication incident data from the incident reporting system (48-months period) and pharmacist interventions data from the prescribing system (26-month period) were extracted. Reason’s Accident Causation Model was used to identify potential causality of the incidents. Pharmacists’ intervention data was thematically analysed.Main outcome measure(a) Frequency, type and potential causality of DOACs incidents, (b) Nature of pharmacists’ interventions.ResultsA total of 812 DOACs reports were included in the study (124 medication incidents and 688 intervention reports). Missing drug/omission was the most common incident type (26.6%,n = 33) followed by wrong drug (16.1%,n = 20) and wrong dose/strength (11.3%,n = 14). A high majority (89.5%,n = 111) of medication incidents were caused by active failures. Patient discharge without anticoagulation supply and failure to restart DOACs post procedure/scan were commonly recurring themes. The majority of (38.1%,n = 262) the pharmacist interventions were related to pharmacological strategy (i.e., drug or dose changes or discontinuation). Impaired renal function was the most common reason for dose adjustments.ConclusionPrescribers’ active failure rather than system errors (i.e. latent failures) are contributing to DOACs incidents. Rreinforcement of guideline adherence, prescriber education, harnessing pharmacists’ roles and mandating renal function information in prescriptions are likely to improve patient safety.
G Protein-Coupled Receptor Kinase 3 Modulates Mesenchymal Stem Cell Proliferation and Differentiation Through Sphingosine-1-Phosphate Receptor Regulation
Authors: Brozowski Jaime , Timoshchenko Roman , Serafin Donald Stephen , Allyn Brittney , Koontz Jessica , Rabjohns Emily , Ren Yinshi , Eudy Amanda , Harris Taylor F. , Abraham David , Mattox Daniel , Rubin Clinton T , Hilton Matthew , Rubin Janet , Allbritton Nancy , Billard Matthew , Tarrant Teresa 
Published: March 2021

Abstract
Background The bone marrow niche supports hematopoietic cell development through intimate contact with multipotent stromal mesenchymal stem cells; however, the intracellular signaling, function, and regulation of such supportive niche cells are still being defined. Our study was designed to understand how G protein receptor kinase 3 (GRK3) affects bone marrow mesenchymal stem cell function by examining primary cells from GRK3-deficient mice, which we have previously published to have a hypercellular bone marrow and leukocytosis through negative regulation of CXCL12/CXCR4 signaling. Methods Murine GRK3-deficient bone marrow mesenchymal stromal cells were harvested and cultured to differentiate into three lineages (adipocyte, chondrocyte, and osteoblast) to confirm multi-potency and compared to wild type cells. Immunoblotting, modified-TANGO experiments, and flow cytometry were used to further examine the effects of GRK3 deficiency on bone marrow mesenchymal stromal cell receptor signaling. Microcomputed tomography was used to determine trabecular and cortical bone composition of GRK3-deficient mice and standard ELISA to quantitate CXCL12 production from cellular cultures.Results GRK3-deficient, bone marrow-derived mesenchymal stem cells exhibit enhanced and earlier osteogenic differentiation in vitro. The addition of a sphingosine kinase inhibitor abrogated the osteogenic proliferation and differentiation, suggesting sphingosine-1-phosphate receptor signaling was a putative G-protein coupled receptor regulated by GRK3. Immunoblotting showed prolonged ERK1/2 signaling after stimulation with sphingosine-1-phosphate in GRK3-deficient cells, and modified-TANGO assays suggested the involvement of β-arrestin-2 in sphingosine 1 phosphate receptor internalization. Conclusions Our work suggests that GRK3 regulates sphingosine-1-phosphate receptor signaling on bone marrow mesenchymal stem cells by recruiting β-arrestin to the occupied GPCR to promote internalization, and lack of such regulation affects mesenchymal stem cell functionality.