bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
Authors: Kodama Joe , Harumningtyas Anjar Anggraini , Ito Tomoko , Michlíček Miroslav , Sugimoto Satoshi , Kita Hidekazu , Chijimatsu Ryota , Ukon Yuichiro , Kushioka Junichi , Okada Rintaro , Kamatani Takashi , Hashimoto Kunihiko , Tateiwa Daisuke , Tsukazaki Hiroyuki , Nakagawa Shinichi , Takenaka Shota , Makino Takahiro , Sakai Yusuke , Nečas David , Zajíčková Lenka , Hamaguchi Satoshi , Kaito Takashi 
Published: September 2021

AbstractRegeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH4/N2/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.
One-transistor static random-access memory cell array comprising single-gated feedback field-effect transistors
Authors: Choi Sangik , Son Jaemin , Cho Kyoungah , Kim Sangsig 
Published: September 2021

AbstractIn this study, we fabricated a 2 × 2 one-transistor static random-access memory (1T-SRAM) cell array comprising single-gated feedback field-effect transistors and examined their operation and memory characteristics. The individual 1T-SRAM cell had a retention time of over 900 s, nondestructive reading characteristics of 10,000 s, and an endurance of 108 cycles. The standby power of the individual 1T-SRAM cell was estimated to be 0.7 pW for holding the “0” state and 6 nW for holding the “1” state. For a selected cell in the 2 × 2 1T-SRAM cell array, nondestructive reading of the memory was conducted without any disturbance in the half-selected cells. This immunity to disturbances validated the reliability of the 1T-SRAM cell array.
Development of an algorithm for assessing fall risk in a Japanese inpatient population
Authors: Nakanishi Tomoko , Ikeda Tokunori , Nakamura Taishi , Yamanouchi Yoshinori , Chikamoto Akira , Usuku Koichiro 
Published: September 2021

AbstractFalling is a representative incident in hospitalization and can cause serious complications. In this study, we constructed an algorithm that nurses can use to easily recognize essential fall risk factors and appropriately perform an assessment. A total of 56,911 inpatients (non-fall, 56,673; fall; 238) hospitalized between October 2017 and September 2018 were used for the training dataset. Correlation coefficients, multivariable logistic regression analysis, and decision tree analysis were performed using 36 fall risk factors identified from inpatients. An algorithm was generated combining nine essential fall risk factors (delirium, fall history, use of a walking aid, stagger, impaired judgment/comprehension, muscle weakness of the lower limbs, night urination, use of sleeping drug, and presence of infusion route/tube). Moreover, fall risk level was conveniently classified into four groups (extra-high, high, moderate, and low) according to the priority of fall risk. Finally, we confirmed the reliability of the algorithm using a validation dataset that comprised 57,929 inpatients (non-fall, 57,695; fall, 234) hospitalized between October 2018 and September 2019. Using the newly created algorithm, clinical staff including nurses may be able to appropriately evaluate fall risk level and provide preventive interventions for individual inpatients.
Broadband infrared light source by simultaneous parametric down-conversion
Authors: Hojo Masayuki , Tanaka Koichiro 
Published: September 2021

AbstractSpontaneous parametric down-conversion is an essential tool for a quantum light source in the infrared region ranging 2–5 µm for the purpose of material identification, chemical analysis, and gas sensing. So far, photon pairs from the process in a nonlinear crystal have low tunability and a narrow spectral range because of the phase-matching condition. Here, we propose a novel type of spontaneous parametric down-conversion processes that overcomes these challenges, where two photon pairs are simultaneously produced in the visible and infrared regions in periodically poled stoichiometric lithium tantalite. It allows broadband and tunable generation of infrared photon pairs that can be employed as an alternative light source for quantum infrared spectroscopy.
Utilizing patient-specific 3D printed guides for graft reconstruction in thoracoabdominal aortic repair
Authors: Kim Taehun , Hong Dayeong , Ock Junhyeok , Park Sung Jun , Rhee Younju , Lee Sangwook , Kim Guk Bae , Yang Dong Hyun , Kim Joon Bum , Kim Namkug 
Published: September 2021

AbstractIn thoracoabdominal aortic aneurysm repair, repairing the visceral and segmental arteries is challenging. Although there is a pre-hand-sewn and multi-branched graft based on the conventional image-based technique, it has shortcomings in precisely positioning and directing the visceral and segmental arteries. Here, we introduce two new reconstruction techniques using patient-specific 3D-printed graft reconstruction guides: (1) model-based technique that presents the projected aortic graft, visualizing the main aortic body and its major branches and (2) guide-based technique in which the branching vessels in the visualization model are replaced by marking points identifiable by tactile sense. We demonstrate the effectiveness by evaluating conventional and new techniques based on accuracy, marking time requirement, reproducibility, and results of survey to surgeons on the perceived efficiency and efficacy. The graft reconstruction guides cover the segmentation, design, fabrication, post-processing, and clinical application of open surgical repair of thoracoabdominal aneurysm, and proved to be efficient for accurately reconstructing customized grafts.
De novo transcriptome assembly, polymorphic SSR markers development and population genetics analyses for southern corn rust (Puccinia polysora)
Authors: Sun Qiuyu , Liu Jie , Zhang Keyu , Huang Chong , Li Leifu , Dong Jiayu , Luo Yong , Ma Zhanhong 
Published: September 2021

AbstractSouthern corn rust is a destructive maize disease caused by Puccinia polysora Underw that can lead to severe yield losses. However, genomic information and microsatellite markers are currently unavailable for this disease. In this study, we generated a total of 27,295,216 high-quality cDNA sequence reads using Illumina sequencing technology. These reads were assembled into 17,496 unigenes with an average length of 1015 bp. The functional annotation indicated that 8113 (46.37%), 1933 (11.04%) and 5516 (31.52%) unigenes showed significant similarity to known proteins in the NCBI Nr, Nt and Swiss-Prot databases, respectively. In addition, 2921 (16.70%) unigenes were assigned to KEGG database categories; 4218 (24.11%), to KOG database categories; and 6,603 (37.74%), to GO database categories. Furthermore, we identified 8,798 potential SSRs among 6653 unigenes. A total of 9 polymorphic SSR markers were developed to evaluate the genetic diversity and population structure of 96 isolates collected from Guangdong Province in China. Clonal reproduction of P. polysora in Guangdong was dominant. The YJ (Yangjiang) population had the highest genotypic diversity and the greatest number of the multilocus genotypes, followed by the HY (Heyuan), HZ (Huizhou) and XY (Xinyi) populations. These results provide valuable information for the molecular genetic analysis of P. polysora and related species.
Rat model of an autologous cancellous bone graft
Authors: Hamada Tomo , Matsubara Hidenori , Hikichi Toshifumi , Shimokawa Kanu , Tsuchiya Hiroyuki 
Published: September 2021

AbstractAutologous cancellous bone (ACB) grafting is the “gold standard” treatment for delayed bone union. However, small animal models for such grafts are lacking. Here, we developed an ACB graft rat model. Anatomical information regarding the iliac structure was recorded from five rat cadavers (10 ilia). Additionally, 5 and 25 rats were used as controls and ACB graft models, respectively. A defect was created in rat femurs and filled with ACB. Post-graft neo-osteogenic potential was assessed by radiographic evaluation and histological analysis. Iliac bone harvesting yielded the maximum amount of cancellous bone with minimal invasiveness, considering the position of parailiac nerves and vessels. The mean volume of cancellous bone per rat separated from the cortical bone was 73.8 ± 5.5 mm3. Bone union was evident in all ACB graft groups at 8 weeks, and new bone volume significantly increased every 2 weeks (P < 0.001). Histological analysis demonstrated the ability of ACB grafts to act as a scaffold and promote bone union in the defect. In conclusion, we established a stable rat model of ACB grafts by harvesting the iliac bone. This model can aid in investigating ACB grafts and development of novel therapies for bone injury.
Structural, optical and microwave dielectric properties of Ba(Ti1−xSnx)4O9, 0 ≤ x ≤ 0.7 ceramics
Authors: Ali Asad , Uddin Sarir , Lal Madan , Zaman Abid , Iqbal Zafar , Althubeiti Khaled 
Published: September 2021

AbstractSn-doped BaTi4O9(BT4) dielectric ceramics were prepared by a mixed oxide route. Preliminary X-ray diffraction (XRD) structural study shows that the ceramic samples have orthorhombic symmetry with space group (Pnmm). Scanning electron microscopy (SEM) shows that the grain size of the samples decreases with an increase in Sn4+content. The presence of the metal oxide efficient group was revealed by Fourier transform infrared (FTIR) spectroscopy. The photoluminescence spectra of the ceramic samples reported red color ~ 603, 604, 606.5 and 605 nm with excitation energy ~ 2.06, 2.05, 2.04 and 2.05 eV for Sn4+content with x = 0.0, 0.3, 0.5, and 0.7, respectively. The microwave dielectric properties of these ceramic samples were investigated by an impedance analyzer. The excellent microwave dielectric properties i.e. high dielectric constant (εr = 57.29), high-quality factor (Qf = 11,852), or low-dielectric loss (3.007) has been observed.
Recent advances of MXenes as electrocatalysts for hydrogen evolution reaction
Authors: Bai Saishuai , Yang Meiqing , Jiang Jizhou , He Xiaomiao , Zou Jing , Xiong Zhiguo , Liao Guodong , Liu Song 
Published: September 2021

AbstractMXenes, an emerging two-dimensional (2D) transition metal carbides, nitrides and carbonitrides, have exhibited great potential as electrocatalysts for hydrogen evolution reaction (HER) due to the excellent characters, including excellent structural and chemical stability, superior electrical conductivity, and large active surface area. In this comprehensive study, firstly, the preparation advances of MXenes are systematically summarized. Then, the representative applications of MXenes-based HER electrocatalysts are introduced, from experimental and theoretical aspects. Thirdly, the strategies for improving HER catalytic activity of MXenes are demonstrated, such as optimizing active sites by termination modification and metal-atom doping, increasing active sites by fabricating various nanostructures. Finally, the existing challenges and new opportunities for MXenes-based electrocatalysts are also elucidated. This paper provides reference for the future development of new and efficient MXenes-based electrocatalysts for hydrogen production through water-splitting technology.
Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems
Authors: Laursen Tomas , Lam Hiu Yue Monatrice , Sørensen Kasper Kildegaard , Tian Pengfei , Hansen Cecilie Cetti , Groves Jay T. , Jensen Knud Jørgen , Christensen Sune M. 
Published: September 2021

AbstractPlant metabolism depends on cascade reactions mediated by dynamic enzyme assemblies known as metabolons. In this context, the cytochrome P450 (P450) superfamily catalyze key reactions underpinning the unique diversity of bioactive compounds. In contrast to their soluble bacterial counterparts, eukaryotic P450s are anchored to the endoplasmic reticulum membrane and serve as metabolon nucleation sites. Hence, membrane anchoring appears to play a pivotal role in the evolution of complex biosynthetic pathways. Here, a model membrane assay enabled characterization of membrane anchor dynamics by single molecule microscopy. As a model system, we reconstituted the membrane anchor of cytochrome P450 oxidoreductase (POR), the ubiquitous electron donor to all microsomal P450s. The transmembrane segment in the membrane anchor of POR is relatively conserved, corroborating its functional importance. We observe dynamic colocalization of the POR anchors in our assay suggesting that membrane anchoring might promote intermolecular interactions and in this way impact assembly of metabolic multienzyme complexes.
Comparative genomic analysis of Methanimicrococcus blatticola provides insights into host adaptation in archaea and the evolution of methanogenesis
Authors: Thomas Courtney M , Taib Najwa , Gribaldo Simonetta , Borrel Guillaume 
Published: September 2021

Abstract
Other than the Methanobacteriales and Methanomassiliicoccales, the characteristics of archaea that inhabit the animal microbiome are largely unknown. Methanimicrococcus blatticola, a member of the Methanosarcinales, currently reunites two unique features within this order: it is a colonizer of the animal digestive tract and can only reduce methyl compounds with H2 for methanogenesis, a increasingly recognized metabolism in the archaea and whose origin remains debated. To understand the origin of these characteristics, we have carried out a large-scale comparative genomic analysis. We infer the loss of more than a thousand genes in M. blatticola, by far the largest genome reduction across all Methanosarcinales. These include numerous elements for sensing the environment and adapting to more stable gut conditions, as well as a significant remodeling of the cell surface components likely involved in host and gut microbiota interactions. Several of these modifications parallel those previously observed in phylogenetically distant archaea and bacteria from the animal microbiome, suggesting large-scale convergent mechanisms of adaptation to the gut. Strikingly, M. blatticola has lost almost all genes coding for the H4MPT methyl branch of the Wood–Ljungdahl pathway (to the exception of mer), a phenomenon never reported before in any member of Class I or Class II methanogens. The loss of this pathway illustrates one of the evolutionary processes that may have led to the emergence of methyl-reducing hydrogenotrophic methanogens, possibly linked to the colonization of organic-rich environments (including the animal gut) where both methyl compounds and hydrogen are abundant.
Twitter, Telepractice, and the COVID-19 Pandemic: A Social Media Content Analysis
Authors: Weidner Kristen , Lowman Joneen , Fleischer Anne , Kosik Kyle , Goodbread Peyton , Chen Benjamin , Kavuluru Ramakanth 
Published: September 2021

PurposeTelepractice was extensively utilized during the COVID-19 pandemic. Little is known about issues experienced during the wide-scale rollout of a service delivery model that was novel to many. Social media research is a way to unobtrusively analyze public communication, including during a health crisis. We investigated the characteristics of tweets about telepractice through the lens of an established health technology implementation framework. Results can help guide efforts to support and sustain telehealth beyond the pandemic context.MethodWe retrieved a historical Twitter data set containing tweets about telepractice from the early months of the pandemic. Tweets were analyzed using a concurrent mixed-methods content analysis design informed by the nonadoption, abandonment, scale-up, spread, and sustainability (NASSS) framework.ResultsApproximately 2,200 Twitter posts were retrieved, and 820 original tweets were analyzed qualitatively. Volume of tweets about telepractice increased in the early months of the pandemic. The largest group of Twitter users tweeting about telepractice was a group of clinical professionals. Tweet content reflected many, but not all, domains of the NASSS framework.ConclusionsTwitter posting about telepractice increased during the pandemic. Although many tweets represented topics expected in technology implementation, some represented phenomena were potentially unique to speech-language pathology. Certain technology implementation topics, notably sustainability, were not found in the data. Implications for future telepractice implementation and further research are discussed.
Residual Tumor Volume and Tumor Progression after Subtotal Resection and Observation of WHO Grade I Skull Base Meningiomas
Authors: Przybylowski Colin J. , Hendricks Benjamin K. , Furey Charuta G. , DiDomenico Joseph D. , Porter Randall W. , Sanai Nader , Almefty Kaith K. , Little Andrew S. 
Published: September 2021

Abstract
Objective This study investigated the impact of residual tumor volume (RTV) on tumor progression after subtotal resection and observation of WHO grade I skull base meningiomas.
Study Design This study is a retrospective volumetric analysis.
Setting This study was conducted at a single institution.
Participants Patients who underwent subtotal resection of a WHO grade I skull base meningioma and postsurgical observation (July 1, 2007–July 1, 2017).
Main Outcome Measure The main outcome was radiographic tumor progression.
Results Sixty patients with residual skull base meningiomas were analyzed. The median (interquartile range) RTV was 1.3 (5.3) cm3. Tumor progression occurred in 23 patients (38.3%) at a mean duration of 28.6 months postsurgery. The 1-, 3-, and 5-year actuarial progression-free survival (PFS) rates were 98.3, 58.6, and 48.7%, respectively. The Cox multivariate analysis identified increasing RTV (p = 0.01) and history of more than 1 previous surgery (p = 0.03) as independent predictors of tumor progression. In a Kaplan–Meier analysis for PFS, the RTV threshold of 3 cm3 maximized log-rank testing significance between groups of patients dichotomized at 0.5 cm3 thresholds (p < 0.01). The 3-year actuarial PFS rates for meningiomas with RTV ≤3 cm3 and >3 cm3 were 76.2 and 32.1%, respectively. When RTV >3 cm3 was entered as a covariate in the Cox model, it was the only factor independently associated with tumor progression (p < 0.01).
Conclusion RTV was associated with tumor progression after subtotal resection of WHO grade I skull base meningioma in this cohort. An RTV threshold of 3 cm3 was identified that minimized progression of the residual tumor when gross total resection was not safe or feasible.
Non-Nutritive Sweeteners in Human Amniotic Fluid and Cord Blood: Evidence of Transplacental Fetal Exposure
Authors: Halasa Brianna C. , Sylvetsky Allison C. , Conway Ellen M. , Shouppe Eileen L. , Walter Mary F. , Walter Peter J. , Cai Hongyi , Hui Lisa , Rother Kristina I. 
Published: September 2021

Objective This study aimed to investigate human fetal exposure to non-nutritive sweeteners (NNS) by analyzing amniotic fluid and umbilical cord blood.
Study Design Concentrations of four NNS (acesulfame-potassium [ace-K], saccharin, steviol glucuronide, and sucralose) were measured in amniotic fluid (n = 13) and cord blood samples (n = 15) using liquid chromatography-mass spectrometry. Amniotic fluid samples were obtained for research purposes at the time of term elective cesarean birth or clinically indicated third trimester amnioreduction at Mercy Hospital for Women (Melbourne, Australia). All except four women were in the fasting state. Cord blood samples were obtained from an independent cohort of newborns whose mothers were enrolled in a separate clinical trial at the National Institutes of Health.
Results Ten of 13 amniotic fluid samples contained at least one NNS (ace-K, saccharin, steviol glucuronide, and/or sucralose). Maximum amniotic fluid NNS concentrations of ace-K, saccharin, steviol glucuronide, and sucralose were 78.9, 55.9, 93.5, and 30.6 ng/mL, respectively. Ace-K and saccharin were present in 100% and 80% of the cord blood samples, with maximal concentrations of 6.5 and 2.7 ng/mL, respectively. Sucralose was not detected and steviol glucuronide was not measurable in any of the cord blood samples.
Conclusion Our results provide evidence of human transplacental transmission of NNS. Based on results predominantly obtained from rodent models, we speculate that NNS exposure may adversely influence the offsprings' metabolic health. Well-designed, prospective clinical trials are necessary to understand the impact of NNS intake during pregnancy on human development and long-term health.
Key Points
Microfluidic high-throughput single-cell mechanotyping: Devices and applications
Authors: Choi Gihoon , Tang Zifan , Guan Weihua 
Published: September 2021

The mechanical behavior of individual cells plays an important role in regulating various biological activities at the molecular and cellular levels. It can serve as a promising label-free marker of cells’ physiological states. In the past two decades, several techniques have been developed for understanding correlations between cellular mechanical changes and human diseases. However, numerous technical challenges remain with regard to realizing high-throughput, robust, and easy-to-perform measurements of single-cell mechanical properties. In this paper, we review the emerging tools for single-cell mechanical characterization that are provided by microfluidic technology. Different techniques are benchmarked by considering their advantages and limitations. Finally, the potential applications of microfluidic techniques based on cellular mechanical properties are discussed.
Double E-shaped toroidal metasurface with high Q-factor Fano resonance and electromagnetically induced transparency
Authors: Chen Ting , Xiang Tianyu , Wang Jianwei , Lei Tao , Lu Fushan 
Published: September 2021

A double E-shaped toroidal dipole metasurface is designed with the high Q-factor Fano and classical electromagnetically induced transparency (EIT) phenomena in the microwave frequency range. With the introduction of an asymmetric structure, the sharp Fano resonance can be excited and acquired a quite high Q-factor of 134 at a lower frequency of 4.58 GHz. It can be numerically and experimentally demonstrated that the singularity Fano response of designed construction is caused by the intensive toroidal dipole. In addition, due to destructive interference between the intensive toroidal dipole and electric dipole, the transmission peak of EIT can reach 0.95 with a Q-factor of 50 at 10.18 GHz. By calculating and comparing the radiated power of multipoles, the enhanced toroidal dipole response can be further verified. The designed planar toroidal dipole metamaterial with simple construction may have many possible applications in toroidal moment generators, sensing, and slow-light devices.