bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low-molecular-weight fibroblast growth factor-2 — a viable prognostic factor for gastric gastrointestinal stromal tumors
Authors: Mikheeva E G , Aukhadieva A M , Sabirov A G , Boichuk S V 
Published: June 2021

Aim. To examine the expression of fibroblast growth factor-2 and its isoforms in gastrointestinal stromal tumors and assess the prognostic value of this marker.
Methods. The study included 44 patients with gastric gastrointestinal stromal tumors of the stomach who were prescribed surgical or combined treatment with the targeted drug imatinib (imatinib mesylate). Immunohistochemistry (IHC)-staining and immunoblotting with monoclonal antibodies were used to assess the expression of FGF-2. Statistical analysis for differences in clinical and morphological parameters was performed by using Students, MannWhitneyWilcoxon and Fishers tests. Differences were considered significant at p 0.05.
Results. Fibroblast growth factor-2 expression was assessed in tumor tissues in 39 out of 44 analyzed patients. The frequency of fibroblast growth factor-2 expression in the observed patients was 84.6% (33/39). The moderate and strong fibroblast growth factor-2 expression was detected in 21 (53.8%) patients with gastric gastrointestinal stromal tumors. High expression of low-molecular weight (18 kDa) fibroblast growth factor-2 isoform was found in all tumor samples from patients with high-risk gastrointestinal stromal tumor (prognostic group 6) (p=0.039), which indicated the active secretion of this ligand by its signalling pathway in the cancer cells. Patients with high levels of low‐molecular‐weight fibroblast growth factor-2 showed a higher level of Ki-67 proliferative activity (р=0.013) and tumor size (р=0.0017). Patients with increased expression of the low molecular weight isoform of fibroblast growth factor-2 in gastric gastrointestinal stromal tumor had a higher risk of recurrence, as well as larger tumor size and proliferative activity compared with patients without expression of this isoform. The level of fibroblast growth factor-2 expression in tumor samples, determined by immunohistochemistry-staining, increases after initiation of imatinib to based therapy, which may indicate the formation of resistance to this targeted drug and the progression of the disease.
Conclusion. The results of the study suggest that FGF-2 might be an independent prognostic marker of gastric gastrointestinal stromal tumor and a viable therapeutic target.
Inclusion of the prepared patient relatives in the process of early rehabilitation of onco-surgical patients in the department of anesthesiology and intensive care
Authors: Saetgaraev A K , Maximov I L , Zakirov I I , Shaymardanov I V , Grigoreva I A , Guryleva M E 
Published: June 2021

Aim. To study the influence of the trained environment of geriatric onco-surgical patients on the quality of the interventions in the complex of early postoperative rehabilitation in the Intensive Care Unit (ICU).
Methods. The 96 patients aged 73.37.1 years operated on in the emergency onco-surgery clinic of the Tatarstan Cancer Center of the Ministry of Health of the Republic of Tatarstan between 2016 and 2020. Patients, similar in age and condition (American Society of Anaesthesiologists Physical Status category IIIE) were divided into three equal groups: the first group generally accepted measures for early rehabilitation were carried out by medical staff; the second group the prepared relatives of the patient was additionally involved in rehabilitation; the control group early rehabilitation was not carried out. The quality of nutritional support and measures to prevent pressure ulcers, cognitive function were assessed. Statistical processing was performed by using Microsoft Excel 2007 and IBM SPSS Statistics version 20 software. The statistical significance of data was determined by using the chi-square test at p 0.05.
Results. The involvement of previously trained relatives made it possible to reduce the incidence of pressure ulcers grade IV to 0%. There was a significant difference in the volume of assimilated enteral oral feeding (180.620.8; 240.620.8; 80.510.2; p 0.05) and the serum albumin level by 11 days after surgery (26.81.5; 28.91.2; 24.21.1 g/L, respectively; p 0.05). Assimilation of enteral oral feeding improved 3 times compared with the control group and 1.5 times compared with the first group (p 0.05). Indicators of cognitive status by the Mini Mental State Examination (MMSE) on the 6th day were 23.31.1; 25.31.1; 21.21.2 (p 0.05), respectively.
Conclusion. A rationally organized preparation of the patient's environment for the implementation of care, participation in rehabilitation measures and the prevention of complications can improve outcomes of medical intervention and reduce the incidence of postoperative complications.
Wellness and Motivation in a Physical Education Course Over a Semester: Examining the Interaction Effects that Motivation has on Wellness Outcomes
Authors: Lothes II John , Kantor Rachel 
Published: June 2021

Objective: This study reports the results that physical education classes have on college students' overall wellbeing and motivation over the course of a semester. This study examines the pre/post outcomes of a physical education course on 12 dimensions of wellness and motivation from the start of the semester to the end of the semester.Participants: College students (N = 1193) taking Physical Education 101 (PED101) over the course of a semester.Methods: Beginning (pre) and end (post) of the semester assessments for wellbeing and motivation were conducted using the Wellness Inventory. Quantitative outcomes from the Wellness Inventory were compared pre/post as well as demographic variables (e.g., gender & exercise frequency). Regression analysis was also conducted to examine how motivation may affect wellness. Results: All 12 dimensions of wellness and motivation showed increases from the start of the semester to the end of the semester. Motivation was also positively correlated with increases in wellness scores. The results found statistically significant changes between the pre- and post-assessments for all dimensions of wellness outcomes and motivation scores. There were also significant changes between the pre- and post-assessments when investigating differences based on sex.Conclusion: PED101 courses can be useful interventions to help move college students towards wellness.
CAMERA EAT FIRST: TOURIST MOTIVATION IN SHARING FOOD PHOTOGRAPH ON INSTAGRAM
Authors: Wachyuni Suci Sandi , Yusuf Lishia 
Published: June 2021

Purpose of the study: Uploading food photos on social media has become a phenomenon among tourists during culinary tours. This phenomenon is increasingly developing into a hobby, namely distributing the art of food photography which is also supported by the development of increasingly sophisticated camera features on smartphones. The purpose of this study is to analyze the motivation of tourists in uploading food photos on Instagram.
Methodology: This research method is descriptive quantitative, and the data collection techniques through distributing questionnaires and literature studies. The sampling technique is non-probability sampling, namely random sampling. The number of respondents in this study was 103 people, and the data analysis technique used descriptive statistics.
Main Findings: This study's theoretical implication shows that the sequence of tourists' motivation to upload food photos on social media is capture togetherness as a realization of the social life of tourists, promotion, food documentation, food art, relaxation, reference, and existence.
Applications of this study: Knowing tourists' motivation can provide an overview of current culinary tourism behavior and can be used as suggestions for culinary businesses in designing marketing strategies. The practical implications are suggestions for culinary entrepreneurs to increase their activity and social media interaction as a marketing effort.
Novelty/Originality of this study: This research is original, and this is the first study that analyzing tourist motivations in sharing food photographs on Instagram. This is new because most studies are mostly done in general consumers, while the context of this study is on culinary tourism.
Homicide Epidemic in Cali, Colombia: A Surveillance System Data Analysis, 1993‒2018
Authors: Guerrero-Velasco Rodrigo , Muñoz Víctor Hugo , Concha-Eastman Alberto , Pretel-Meneses Álvaro J. , Gutiérrez-Martínez Maria I. , Santaella-Tenorio Julian 
Published: June 2021

Objectives. To examine homicide rates in Cali, Colombia, during the 1993–2018 period, using information derived from an interagency surveillance system.Methods. We used homicide data from Cali’s Epidemiological Surveillance System to examine homicide trends by victim’s age and sex, time, and type of method used. We estimated trend changes and the annual percentage changes using joinpoint regression analyses.Results. Homicide rates per 100 000 inhabitants dropped from 102 in 1993 to 47.8 in 2018. We observed reductions in homicide rates across age and sex groups. Most homicide victims were men aged 20 to 39 years from poor, marginalized areas. Firearms were used in 84.9% of all cases. The average annual percentage change for the entire period was −3.6 (95% confidence interval = −6.7, −0.4).Conclusions. Fluctuations in homicide rates in Cali show a clear epidemic pattern, occurring concurrently with the “crack epidemic” in different countries. Reliable and timely information provided by an Epidemiological Surveillance System allowed opportune formulation of public policies to reduce the impact of violence in Cali.
Implications of Lived and Packaged Religions for Intercultural Dialogue to Reduce Conflict and Terror
Authors: Bouma Gary D 
Published: June 2021

The use of intercultural dialogue (ICD) to promote intergroup understanding and respect is considered as a key to reduce tensions and the likelihood of conflict. This paper argues that understanding the differences among religions – those between packaged and lived religion – enhances the chances of success and makes the effort more challenging. Religions contained and packaged are found in formallyorganised expressions of religion – churches, denominations, synagogues, mosques, temples and so on. For packaged religions, religious identity is singular and adherents are expected to identify with only one religion and are assumed to accept the whole package of that religion. ICD in this context involves communicating with religious groups such as organisations and encouraging different leaders to speak with each other resulting in platforms filled with ‘heads of faith’ – bishops, muftis, ayatollahs, chief rabbis, swamis and so on. In contrast, lived religions involve ritual practices engaged in by individuals and small groups, creation of shrines and sacred spaces, discussing the nature of life, sharing ethical concerns, going on pilgrimages and taking actions to celebrate and sustain hope. There is some evidence that, although packaged religions are declining, lived religions continue at persistent levels. Violent extremism is more likely to be associated with lived rather than packaged forms of religion, making a more balanced intercultural competences approach to ICD critical to countering conflict.1
1 This article is a revised version of Gary D Bouma (2017) ‘Religions – lived and packaged – viewed through an intercultural dialogue prism’ in Fethi Mansouri (ed) Interculturalism at the Crossroads: Comparative perspectives on concepts, policies and practice, United Nations Educational, Scientific and Cultural Organization, France, pp. 129–144.
Bio-Accessibility and Health Risk Assessment of Heavy Metals in Indoor Dust From Higher Institutions in Ondo State, Nigeria
Authors: Ajayi Omoyemi Oluwatosin , Aborode Abdullahi Tunde , Orege Joshua Iseoluwa , Oyewumi Tolulope Oyelekan , Othmani Amina , Adegbola Mary Adeola , Orege Odunola Blessing 
Published: June 2021

Abstract
Quality of indoor air has been a major concern over time particularly in urban areas. Amidst the indoor air parameters, particulate matter/dust is of core interest owing to the fact that it acts as sediment for metals and other pollutants. Considering the ample amount of time spent indoor by students, this study has been mapped out to investigate the quality of air in tertiary institutions in Ondo State, Nigeria based on their proximity to more anthropogenic activities. Herein, pH, conductivity, and anion concentrations in the indoor dust samples were estimated employing standard analytical methods. Our findings revealed a sequence of anion concentration level as follows, Cl−>SO42−>NO3−. Also, the concentrations of some selected metals were analyzed using flame atomic absorption spectrophotometer (FAAS). The variation in metal concentration observed in most study sites was in the order Cu > Zn > Cr > Pb > Cd. The mean heavy metal variation followed the trend based on the sample collection sites considered in the present study with FE > OA > FU > HT and Cu (0.480mg/kg), Zn (0.409mg/kg), Cr (0.407mg/kg), Pb (0.149mg/kg) and Cd (0.029mg/kg) being the highest concentration of each of the metals. However, all the metal concentrations were below the permissible level. The result from non-carcinogenic study indicates that the average daily dose (by ingestion) was found to be the core exposure pathway such that ADDing > ADDderm > ADDinh. Nevertheless, the hazard quotient (HQ) and hazard index (HI) were less than 1. This implies that no potential health risk. More generally, a relative degree of safety and strict regulations is suggested to be put in place to maintain the status.
Deciphering Local Adaptation Of Native Indian Cattle (Bos Indicus) Breeds Using Landscape Genomics And In-Silico Prediction Of Deleterious SNP Effects On Protein Structure And Function
Authors: Bhardwaj Shivam , Singh Sanjeev , Ganguly Indrajit , Bhatia Avnish Kumar , Dixit S. P. 
Published: June 2021

Abstract
Present study aimed to explore genomic basis of adaptation of Indian native cattle and to predict the impact of key SNPs on amino acid changes that affect protein function. Four native cattle breeds belonging to contrasting landscape and climatic conditions were genotyped using Illumina 777 K BovineHD BeadChip: Siri & Ladakhi from cold hilly areas, and Kankrej and Hallikar from hot arid and semi-arid regions, respectively. The R.SamBada package in R was used to perform the genotype-environment association analysis. A total of 1,12,780 significant (q < 0.05), models with 30,350 unique SNPs were obtained. Significantly associated SNPs had impact on 4,435 genes and 141 pathways. Only ten SNP variants had a SIFT score of < 0.05 (deleterious), and only two of them, each lying in the genes CRYBA1 and USP18, were predicted to be deleterious with high confidence.. RaptorX predicted the tertiary structures of proteins encoded by wild and mutant variants of these genes. The quality of the models was determined using Ramachandran plots and RaptorX parameters, indicating that they are accurate. RaptorX and I-Mutant 2.0 softwares revealed significant differences among wild and mutant proteins. Identified adaptive alleles might be responsible for the local adaptation of these cattle breeds.
The Economic Value of the Ecosystem Services of the Papenkuils Wetland – a Wetland in a Dryland
Authors: Horn Annabel Marian , Law Matthew , Lorentz Simon , Day Liz , Dalgliesh Christopher , Reuther Sue , Kloppers Wilna 
Published: June 2021

Abstract
The Wetlands in Drylands presented in this study focussed on the Papenkuils Wetland, which is in the Western Cape of South Africa, north of the Brandvlei Dam, and south west of Worcester, downstream of the confluence of the Breede (or Bree) and Smalblaar Rivers. This study sets out to establish the shadow price of the environmental services of this wetland, and to understand how this value changes with the possible future diversion of water to an agricultural dam before the Wetland, water provision to the wetlands. The study strongly recommends that the functionality of this wetland, as the largest in the Breede (Afrikaans word meaning wide) Catchment and with biodiversity that is recognised as an important remnant of habitats in the region, be maintained through management and sufficient inundation of water. This would be accompanied by a programme of abstraction for irrigation at a pumping station below the Papenkuils Wetland, as opposed to further abstraction of winter water above the wetland, as is currently proposed.
Nanomolar EP4 Receptor Potency and Expression of Eicosanoid- Related Enzymes in Normal Appearing Colonic Mucosa From Patients with Colorectal Neoplasia
Authors: Feddersen Ulrike Ries , Hendel Sebastian Kjærgaard , Berner-Hansen Mark Alexander , Jepps Thomas Andrew , Bindslev Niels , Berner-Hansen Mark 
Published: June 2021

Abstract
BackgroundAberrations in cyclooxygenase and lipoxygenase (LOX) pathways in non-neoplastic, normal appearing mucosa from patients with colorectal neoplasia (CRN), could hypothetically qualify as predisposing CRN-markers. To test this hypothesis, biopsies were obtained during colonoscopy from macroscopically normal colonic mucosa from patients with and without CRN. Prostaglandin E2 (PGE2) receptors, EP1-4, were examined in Ussing-chambers by exposing biopsies to selective EP receptor agonists, antagonists and PGE2. Furthermore, mRNA expression of EP receptors, prostanoid synthases and LOX enzymes were evaluated using qPCR technology.Results Data suggest that PGE2 binds to high and low affinity EP receptors. In particular, PGE2 demonstrated EP4 receptor potency in the low nanomolar range. Similar results were detected using EP2 and EP4 agonists. In CRN patients, mRNA-levels were higher for EP1 and EP2 receptors and for enzymes prostaglandin-I synthase, 5-LOX, 12-LOX and 15-LOX. ConclusionIn conclusion, normal appearing colonic mucosa from CRN patients demonstrates deviating expression in eicosanoid pathways, indicating a likely predisposition for early CRN development. Moreover, PGE2 potency activates high affinity EP4 receptor subtypes, supporting relevance of testing EP4 antagonists in colorectal cancer management.
Underrecognized Sleep Disorders Across Rare Diseases: Real-world Insights From a Patient and Caregiver Summit
Authors: Picone Maria , Naujokas Marisa F. , Gorman Casey , Jesteadt Lindsay , Kelly Erica , Kelly Jeremy , King Rebecca , Maier Steve , O’Shaughnessy Sharon , Pullen Lara C. , Rivard Paige , Strong Theresa V , Pfalzer Anna C , Bichell Terry Jo 
Published: June 2021

Abstract
Background: Disorders of sleep-wake stability have a negative impact on patient and caregiver quality of life. There is evidence that sleep disorders and their sequelae may be an underrecognized burden for people living with rare diseases. Given that the sleep-wake cycle is regulated via the hypothalamus, people with neurological disorders may be particularly affected. To collect real-world insights into the impact of sleep disturbances and excessive daytime sleepiness (EDS) on people with rare disorders, Harmony Biosciences organized a Summit on Sleep Disruption. The attendees were from seven patient advocacy groups, each representing a rare neurological disorder reporting sleep disturbances, specifically EDS: Angelman syndrome, idiopathic hypersomnia, Kleine-Levin syndrome, myotonic dystrophy, narcolepsy, Prader-Willi syndrome, and Smith-Magenis syndrome. One person represented COMBINEDBrain, a patient advocacy consortium representing 25 rare, genetic neurological disorders. Results: The overwhelming response was that disordered sleep, particularly EDS, is a major health problem within their populations that they feel is not adequately diagnosed or treated. Participants felt that burdens associated with sleep disturbances were underrecognized as a treatable medical issue and were not effectively addressed by health care providers. Participants noted the following unmet needs: patient and physician education on sleep disturbance, effective medications approved for these disease populations, and medically acceptable patient-centered devices to measure sleep parameters. Participants described impacts of disordered sleep and EDS on behavior and school performance, describing impaired wakefulness as an interference with normal daytime functioning. Participants also revealed tremendous burdens on the caregivers and family members who live with people experiencing sleep-wake instability. Conclusions: These results provide real-world insights that disordered sleep including EDS and subsequent impaired daytime functioning are major burdens in these rare disease communities. Furthermore, these burdens are underrecognized for caregivers and, for some rare diseases, among the patients themselves. Participants identified gaps in knowledge and treatment of sleep disorders and resolved to continue the dialog to find ways to raise awareness of disordered sleep and reduce burdens in these patient populations.
Evaluation of A Simultaneous Adsorption Device For Cytokines And Neutrophil-Platelet Complexes In Vitro And In A Lung-Protective Ventilated Rabbit Acute Lung Injury Model
Authors: Sekiya Yumiko , Shimada Kaoru , Takahashi Hiroshi , Kuga Chisa , Komachi Shunsuke , Miwa Keishi , Kotani Toru 
Published: June 2021

Abstract
Background: Neutrophil-platelet complexes (NPCs) readily migrate into tissues and induce tissue damage via cytokine or other pathogenic factors release. These actions are involved in onset and progression of acute respiratory distress syndrome (ARDS). Thus, simultaneous removal of cytokines and activated neutrophils that includes NPCs by blood purification may prevent development of ARDS and enhance drug effects. The goal of this study was to examine the effect of a newly developed adsorption column (NOA-001) that eliminates cytokines and activated neutrophils in a lung injury model.Results: Adsorption of cytokines such as IL-8, IL-6 and HMGB-1, and NPCs was first measured in vitro. Lung injury was induced by HCl and lipopolysaccharide intratracheal infusion in rabbits ventilated at a low tidal volume (7-8 mL/kg) and PEEP (2.5 cmH2O) for lung protection. Arterial blood gas, hematologic values, plasma IL-8, blood pressure and heart rate were measured, and lung damage was evaluated histopathologically in animals treated with 8-hour direct hemoperfusion with or without use of NOA-001. The in vitro adsorption rates for IL-8, IL-6, HMGB-1, activated granulocytes and NPCs were 99.4 ± 0.0164%, 63.6 ± 0.367%, 59.0 ± 1.58%, 7.05 ± 9.63% and 55.7 ± 12.7%, respectively. Absorption of NPCs onto fibers was confirmed microscopically. These adsorption effects were associated with several improvements in the rabbit model. In respiratory function, the PaO2/FIO2 ratios at 8 h were 314 ± 55.3 mmHg in the NOA-001 group and 134 ± 40.8 mmHg in the sham group. The oxygenation index and PaCO2 also differed significantly between the two groups (p<0.05). Acidosis was observed in the sham group, whereas blood pH was maintained within the normal range in the NOA-001 group (p<0.05). In lung histopathology, fewer hyaline membrane and inflammatory cells were observed in the NOA-001 group.Conclusion: A column for simultaneous removal of cytokines and NPCs showed efficacy for improvement of pulmonary function in an animal model. This column may be effective in support of treatment of ARDS.
Multicenter Observational Study on Detection of Atrial Fibrillation Using Insertable Cardiac Monitors in Patients With Cryptogenic Stroke in Japan : LOOK Registry - Study Design and Characteristics
Authors: Suda Satoshi , Katano Takehiro , Kitagawa Kazuo , Iguchi Yasuyuki , Fujimoto Shigeru , Ono Kenjiro , Kano Osamu , Takekawa Hidehiro , Koga Masatoshi , Ihara Masafumi , Morimoto Masafumi , Yamagami Hiroshi , Terasaki Tadashi , Yamaguchi Keiji , Okubo Seiji , Ueno Yuji , Ohara Nobuyuki , Kamiya Yuki , Takeuchi Masataka , Yazawa Yukako , Terasawa Yuka , Doijiri Ryosuke , Tsuboi Yoshifumi , Sonoda Kazutaka , Nomura Koichi , Shimoyama Takashi , Kutsuna Akihito , Kimura Kazumi 
Published: June 2021

Abstract
The authors have requested that this preprint be removed from Research Square.