bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of Rockburst Prevention in Deep Thick Coal Seams with Cemented Paste Backfill: A Case Study
Authors: Chang Qingliang , Leng Qiang , Sun Xikui , Li Guichen , Zhang Biao 
Published: November 2021

Abstract
The aim of this study is to explore a safe mining method to effectively excavate the deep thick coal seams in rockburst-prone mines. Based on the theory of elastic foundation and geological conditions of the Xinhe Coal Mine, the cemented paste backfill (CPB) is proposed to prevent rockburst. In this study, the roof fracture mechanism of block caving mining (BCM) and CPB methods are established. Then, the stress evolution of the surrounding rock and the subsidence of roof strata with these two methods are compared. The results show that the maximum bending moment appears in the middle of the roof, and the value is far below the critical bending moment of the roof by using the CBP. While using the BCM, this value exceeds the critical bending moment of the roof, which may trigger rockburst-related problems. In addition, there is no first weighting and periodic weighting phenomena by using the CPB method as the overburden pressure is gradually transferred to the backfill body, resulting in a safer mining condition. Furthermore, the engineering application indicates that the frequency of daily microseismic events and the burst energy are significantly reduced by using the CPB.
Effect of Biodentine Coated with Emdogain on Proliferation and Differentiation of Stem Cells from the Apical Papilla
Authors: Karkehabadi Hamed , Ahmadyani Erfan , Najafi Rezvan , Khoshbin Elham 
Published: November 2021

Abstract
Background: This study assessed the effect of Biodentine coated with Emdogain (Biodentine/Emdogain) on proliferation and differentiation of stem cells from the apical papilla (SCAP). Methods and Results: In this in vitro, experimental study, SCAP were isolated from two immature impacted third molars and cultured. After ensuring the stemness of the cells by assessing their cell surface markers, they were exposed to Biodentine, Emdogain, and Biodentine/Emdogain for 24 and 72 hours. The control cells did not receive any intervention. Cell viability was evaluated by the methyl thiazolyl tetrazolium (MTT) assay. Expression of odontogenic differentiation genes was analyzed by the quantitative reverse transcription polymerase chain reaction (qRT-PCR). Alkaline phosphatase (ALP) activity was quantified by the respective kit. Data were analyzed by one-way ANOVA, t-test, and Mann-Whitney test (α=0.05). Cell viability did not change after 24 hours of exposure to biomaterials. At 72 hours, the viability of the cells exposed to Biodentine and Biodentine/Emdogain decreased compared with the control group. The expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), and bone sialoprotein (BSP) genes, and ALP activity significantly increased in all three experimental groups, compared with the control group at both 24 and 72 hours; this increase was significantly greater in Biodentine/Emdogain group. The number of mineralized nodules significantly increased in all groups after 72 hours with a greater rate in Biodentine/Emdogain group.Conclusions: All biomaterials increased the differentiation of SCAP, expression of odontogenic genes, and ALP activity, but Biodentine/Emdogain was significantly more effective for this purpose.
Genotyping marker density reduction is not an effective approach in long-term prediction-based breeding of cross-pollinated crops
Authors: DoVale Julio Cesar , Carvalho Humberto Fanelli , Sabadin Felipe , Fritsche-Neto Roberto 
Published: November 2021

Abstract
Reductions of genotyping marker density have been extensively evaluated as potential strategies to reduce the genotyping costs of genomic selection (GS). Low-density marker panels are appealing in GS because they entail lower multicollinearity and computational time-consumption and allow more individuals to be genotyped for the same cost. However, statistical models used in GS are usually evaluated with empirical data, using "static" training sets and populations. This may be adequate for making predictions during a breeding program's initial cycles, but not for the long term. Moreover, to the best of our knowledge, no GS models consider the effect of dominance, which is particularly important for breeding outcomes in cross-pollinated crops. Hence, dominance effects are an important and unexplored issue in GS for long-term programs involving allogamous species. To address it, we employed two approaches: analysis of empirical maize datasets and simulations of long-term breeding applying phenotypic and genomic recurrent selection (intrapopulation and reciprocal schemes). In both schemes, we simulated twenty breeding cycles and assessed the effect of marker density reduction on the population mean, the best crosses, additive variance, selective accuracy, and response to selection with models (additive, additive-dominant, general (GCA), and specific combining ability (SCA)). Our results indicate that marker reduction based on linkage disequilibrium levels provides useful predictions only within a cycle, as accuracy significantly decreases over cycles. In the long-term, high-marker density provides the best responses to selection. The model to be used depends on the breeding scheme: additive for intrapopulation and additive-dominant or SCA for reciprocal.
Connection of TF and TCF4 Gene Polymorphisms with ASD
Authors: Azmerin Maria , Hussain Md. Saddam , Islam Md. Siddiqul , Aziz Md. Abdul , Moghal Md. Mizanur Rahman , Begum Mobashera , Sen Niloy , Rahman Md. Abdur , Shahriar Mohammad , Ash Ghulam Md , Islam Mohammad Safiqul 
Published: November 2021

Abstract
Though the prevalence of autism spectrum disorder (ASD) is increasing day by day, there is still a lack of a proper way to diagnose or prevent ASD. There is no study carried out in the Bangladeshi children with ASD to evaluate the association of Transferrin (TF) and Transcription Factor 4 (TCF4) genetic polymorphisms. This genetic association study was designed to explore the association of rs1867503 polymorphism of TF and rs9951150 polymorphism of TCF4 genes with ASD. We collected blood from 96 children with ASD and 118 healthy children of very similar age differences. Genotyping of these SNPs was performed by the PCR-RFLP method. SPSS (version 16) was used to estimate the odds ratio (OR) and their 95% confidence intervals (CI). The frequency of mutant allele G for rs1867503 and rs9951150 polymorphisms was found 48% and 44%, respectively. In our analysis, both TF and TCF4 polymorphisms showed an increased risk for the development of ASD. AG heterozygote, GG mutant homozygote, AG+GG combined genotype, and G mutant allele of TF rs1867503 showed a significantly elevated risk of ASD development (OR=3.18, p=0.0003; OR=2.62, p=0.0128; OR=2.98, p=0.0002; and OR=1.94, p=0.001, respectively). Likewise, AG heterozygote, GG mutant homozygote, AG+GG combined genotype, and G minor allele of TCF4 rs9951150 also showed a significantly elevated risk of ASD development (OR=2.92, p=0.0007; OR=2.36, p=0.0273; OR=2.72, p=0.0005; and OR=1.92; p=0.0014, respectively). Our results indicate that TF rs1867503 and TCF4 rs9951150 polymorphisms are strongly associated with the development of ASD in Bangladeshi children.
Differential Effects of Bariatric Surgery on Plasma Levels of ANGPTL3 and ANGPTL4.
Authors: Bini Simone , D’Erasmo Laura , Astiarraga Brenno , Minicocci Ilenia , Palumbo Maria , Pecce Valeria , Polito Luca , Costanzo Alessia , Haeusler Rebecca A. , Arca Marcello , Ferrannini Ele , Camastra Stefania 
Published: November 2021

Abstract
Introduction: Angiopoietin-like protein 3 (ANGPTL3) and 4 (ANGPTL4) are regulators of triglycerides storage and utilization. Bariatric surgery (BS) determines profound changes in adipose tissue composition and energy metabolism. We evaluated the impact of BS on plasma levels of ANGPTL3 and ANGPTL4.Method: Twenty-seven morbidly obese subjects with or without type 2 diabetes (T2D) underwent Roux-en-Y gastric bypass (RYGB) and 18 patients with advanced T2D received Biliopancreatic Diversion (BPD). Fasting ANGPTLs levels, insulin sensitivity (evaluated by euglycemic hyperinsulinemic clamp), total bile acids (TBA) and free fatty acids (FFA) were measured at baseline and 1year after surgery.Results: Both surgical procedures resulted in fat mass loss, improved glucose control, and a ~2-fold increase of insulin sensitivity. ANGPTL4 levels decreased significantly with both RYGB (26.6 ± 0.6 to 24.4 ± 0.3 ng/mL, p=0.001) and BPD (27.9 ± 1.5 to 24.0 ± 0.5 ng/mL, p=0.003). In contrast, ANGPTL3 concentrations did not change after RYGB but rose following BPD (225 ± 20 to 300 ± 15 ng/mL, p=0.003). By multiple regression analysis, changes in ANGPTL4 were independently associated with those of blood glucose, p=0.0169) whereas changes in ANGPTL3 after BPD were associated with variations in FFA (p=0.008) and insulin sensitivity (p=0.0427). Discussion: Circulating ANGPTL4 is reduced by BS probably due to the loss of fat mass and improved insulin sensitivity. Conversely, ANGPTL3 levels increased after BPD, presumably because of the metabolic changes induced by the malabsorptive effect of this surgical procedure.
Characterization and Biological Activity of a Novel Exopolysaccharide Produced By Pediococcus Pentosaceus SSC-12 From Silage
Authors: Fan Yang , Li Xinqin , Tian Rong , Tang Ruxue , Zhang Jianguo 
Published: November 2021

Abstract
Background: The exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) are widely used in various fields because of their safety and various biological activities. In this study, we extracted and characterized the composition as well as antioxidant and antibacterial activities of EPS from Pediococcus pentosaceus SSC-12 isolated from the silage.Results: The LAB strain SSC-12 was screened and identified as Pediococcus pentosaceus, based upon 16S rDNA gene sequencing and Neighbor Joining (NJ) phylogenetic analysis. The analysis of the EPS production kinetics results of SSC-12 showed that the EPS production reached the maximum at 20 h of culture. High-performance anion exchange chromatography (HPAEC) analysis showed that the EPS produced by SSC-12 was a heteropolysaccharide comprising glucose (42.6 %), mannose (28.9 %), galactose (16.2 %), arabinose (9.4 %) and rhamnose (2.9 %). The EPS had good antioxidant activity, especially hydroxyl radical scavenging activity. When the concentration of the EPS produced by SSC-12 (SSC-12 EPS) was 10 mg/mL, its 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, hydroxyl radical scavenging ability, superoxide scavenging ability, and reduction ability were 77.4 %, 97.5 %, 77.5 % and 1.3, respectively. At 10 mg/mL，SSC-12 EPS completely killed Staphylococcus aureus GDMCC 1.1220 and substantially inhibited the growth of Salmonella enterica subsp. enterica GDMCC 1.345; however, it had a weak inhibitory effect on Listeria monocytogenes GDMCC 1.347. Conclusions: Due to its strong antioxidant and antibacterial properties, EPS produced by LAB strain SSC-12 have potential application as a bioactive product in the feed, food, and pharmaceutical industries.
Cost-effectiveness of using protons in breast cancer patients aiming at reducing cardiotoxicity: A risk-stratification analysis
Authors: Li Guo , Xia Yun-Fei , Huang Yi-Xiang , Okat Deniz , Qiu Bo , Doyen Jerome , Bondiau Pierre-Yves , Benezery Karen , Gao Jin , Qian Chao-Nan 
Published: November 2021

Abstract
Purpose

Incidental exposure of heart to ionizing irradiation is associated with an increased risk of ischemic heart disease (IHD) in breast cancer patients after radiotherapy. Intensity-modulated proton radiation therapy (IMPT) offers a promise in limiting the mean heart dose (MHD) in breast irradiation to a negligible level. However, the uncertainty in cost-effectiveness hinders its use. This cost-effectiveness analysis aims to identify patients in appropriate risk groups as targets for IMPT.
Methods

A Markov decision model was designed to evaluate the cost-effectiveness of IMPT versus intensity-modulated photon-radiation therapy (IMRT) in reducing the irradiation-related IHD risk. Baseline evaluation was performed on 50-year-old women patient without preexisting cardiac risk factor (CRF). Stratified for preexisting cardiac risk and photon MHD, cost-effective scenarios under different proton cost and willingness-to-pay (WTP) were identified for 40-, 50- and 60-year-old patients.
Results

With baseline set-ups, incremental effectiveness (IE) ranged from 0.025 quality-adjusted life-year (QALY) to 0.135 QALY when photon MHD varied from 3 to 16 Gy; IE increased from 0.043 QALY to 0.964 QALY when preexisting cardiac risk increased from the baseline level to its 10 times. IMPT was not cost-effective to patients without preexisting CRF. At the WTP of China, once proton cost reduced to $20,000, IMPT would be cost-effective to ≤ 50-year-old women patients having preexisting cardiac risk of general-population level.
Conclusion

Patient’s preexisting cardiac risk level should be a main consideration for the clinical decision of using protons; protons may become cost-effective to general-level patients if a substantial decrease in proton cost occurs in the future.
Isolation of Antibiotic-Resistant Bacteria From The Atmospheric Air In Hospital Wards And Outdoor Areas In Kuwait During Sandstorms
Authors: Shamsah Sara , Vali Leila , Al-Kayyalli Dana , Dashti Ali A. 
Published: November 2021

Abstract
Aim: Antibiotic resistance is a public health concern that is linked to increased mortality, morbidity, extended hospital stays, decreased productivity, and therefore, increased financial implications. The source and dissemination of antimicrobial resistance have been linked to environmental factors, including dust storms. There is clear evidence that there has been a rapid increase in temperature in the past decades which increases the risk of sandstorms in places that had never previously experienced this phenomenon. The aim of this study is to isolate medically important micro-organisms in atmospheric air samples from outdoor spaces and inside hospital wards during sandstorms and to characterize their antimicrobial sensitivity profiles to common antibiotics.Findings: Eighty-four colonies were isolated from the target sites and identified as Staphylococcus aureus, coagulase-negative Staphylococcus, Bacillus spp., Acinetobacter spp., from hospital air samples, and Pseudomonas spp., and Staphylococcus spp. from outdoor air samples. Multi-drug resistance patterns were observed for isolates obtained from both indoor and outdoor air samples. PCR showed 6.5% of S. aureus contained mecA. Conclusion: This finding supports the notion that atmospheric air during dust storms can be counted as a source of antibiotic resistant bacteria which merits more attention especially with global warming and climate change contributing to extreme weather events.
Effects of Biofilm Burkholderia Pyrrocinia JK-SH007 On The Colonization And Growth Promotion of Poplar
Authors: Fu Huanhuan , Kong Weiliang , Chen Feifei , Liu Wanhui , Xu Qianru , Wang Chaoen , Fang Xueqi , Ye Jianren 
Published: November 2021

Abstract
Burkholderia pyrrocinia JK-SH007 is a high-potential biological control strain. We changed the composition of medium during the fermentation of JK-SH007 cells and induced these cells to form a biofilm. In this experiment, we deeply studied the biofilm physical and chemical properties. The new fermentation process improves the colonization ability of JK-SH007 and promotes poplar growth. In addition, the biofilm bacterial concentration reached 1010 CFU/mL, the cell dry weight increased over that of a control by 3-10-fold, there was increased environmental stress resistance and IAA secretion, and progeny cells retained resistance to adverse environments. The new biofilm cells were applied to poplar. The JK-SH007 colonization ability was improved in the biofilm, and some bacteria existed as biofilms (cell clusters) in poplar, which would promote forming a dominant niche. Biofilm JK-SH007 has an increased affinity for poplar during colonization and promotes poplar growth under hydroponic conditions, proving the reliability of the new morphology for treating poplar ulcer disease. This work further provides a theoretical basis for commercially producing JK-SH007.
Citrus Peels Phenolic Derivatives Alleviate Benzo[α]pyrene-Induced Inflammatory Injury of Human Intestinal Epithelial Cells Through NLRP3 and AhR Signaling Pathways
Authors: Liang Zhuwei , Wang Huailing , Luo Dan , Liu Xiaoyu , Liu Jie 
Published: November 2021

Abstract
Benzo[α]pyrene (BaP) is ubiquitous in foods, and possesses a fatal cytotoxicity. In current study, ten Citrus peels (Chenpi) phenolic derivatives (CPDs) were isolated in a cell model of human intestinal epithelial (Caco-2) cells under BaP-exposure by a bio-assay guided method. Among them, methyl (3,4,5-trimethoxybenzoyl) valylphenylalaninate (Citrus peels phenolic derivative-2, CPD-2) performed the most protective activity by promoting the antiinflammatory potential on BaP-induced Caco-2 cells. CPD-2 inhibited BaP-induced intracellular ROS over-production and inflammatory epithelial cytokine, IL-4, IL-8, TNF-α, IL-1β and IL-18 over-expression, but not IL-6. CPD-2 also inhibited BaP-induced NLRP3 inflammasome and AhR signaling pathway activation. Overall, CPD-2 attenuates BaP-induced apoptotic death via promoting the antiinflammatory potentials by inhibiting the NLRP3 and AhR signaling pathways activation of Caco-2 cells. Finally, the Citrus peels phenolic derivatives was observed for the first time against BaP-induced inflammation and oxidative stress in human intestinal epithelial cells.
ColabFold - Making protein folding accessible to all
Authors: Mirdita Milot , Schütze Konstantin , Moriwaki Yoshitaka , Heo Lim , Ovchinnikov Sergey , Steinegger Martin 
Published: November 2021

Abstract
ColabFold offers accelerated protein structure and complex predictions by combining the fast homology search of MMseqs2 with AlphaFold2 or RoseTTAFold. ColabFold's 20-30x faster search and optimized model use allows predicting thousands of proteins per day on a server with one GPU. Coupled with Google Colaboratory, ColabFold becomes a free and accessible platform for protein folding. ColabFold is open-source software available at github.com/sokrypton/ColabFold. Its novel environmental databases are available at colabfold.mmseqs.com.
Validity and Application of a Portable Force Platform in Assessment of Dynamic Balance Ability
Authors: Lin Jinpeng , Wang Fang , Zhao Yaqi , Li Junjie , Li Jixin , Tian Fei , Zhai Hua , Wang Shaobai 
Published: November 2021

Abstract
Background: Dynamic balance assessment, which requires a specialized device, is crucial in clinic to evaluate postural control comprehensively. The Nintendo Wii Balance Board (WBB), a portable force platform may be a suitable alternative to the expensive “gold standard”- the laboratory-grade force platform (FP). However, its validity in assessment of dynamic balance is still unclear. The purpose of this study is to demonstrate the validity of the WBB in dynamic balance assessment.Methods: We performed three static and dynamic balance tests, including open eyes single-leg stand, close eyes single-leg stand and Limitation of Stability, on the WBB for 34 healthy participants. Trajectories of center of pressure (COP) were recorded synchronously and used to compute seven characteristics. To quantify the consistency of the two devices, we used intraclass correlation coefficient (ICC) as well as visual evaluation of Bland–Altman plots.Results: The data showed a high consistency between the two devices (ICC = 0.92-0.98) under static and dynamic balance assessments, and the visual evaluation result from Bland–Altman plot was acceptable between device agreement. Moreover, in the dynamic balance task (Limitation of Stability test), the typical ranges of COP-based postural sway distances for healthy adults in medial-lateral and anterior-posterior measured by the WBB were 27.17 ± 3.88 cm and 21.13 ± 2.33 cm, respectively, indicating the validity of the WBB in assessing COP under both static or dynamic balance tasks. Conclusion: With the advantages of portability and low-cost, the valid WBB can facilitate the popularization of quantitative balance evaluation to basic hospitals. Our results provide valuable reference for clinical evaluation of balance ability.
Factors Associated With Low Back Pain In Patients With Lumbar Spinal Stenosis: A Cross-Sectional Study
Authors: Ogon Izaya , Iba Kousuke , Takashima Hiroyuki , Terashima Yoshinori , Yoshimoto Mitsunori , Emori Makoto , Teramoto Atsushi , Takebayashi Tsuneo , Yamashita Toshihiko 
Published: November 2021

Abstract
Background: Low back pain (LBP) is a major symptom of lumbar spinal stenosis (LSS). To develop better treatment, it is important to assess LBP in patients with LSS. This study aimed to analyze the factors associated with LBP in patients with LSS.Methods: This cross-sectional study included consecutive patients with LSS aged between 51 and 79 years who had symptoms in one or both legs with or without LBP. The participants were classified into two groups: the high group (LBP visual analog scale [VAS] score of 30 mm or more) and the low group (LBP VAS score of less than 30 mm). We performed multiple logistic regression analysis with the high and low groups as dependent variables and a receiver operating characteristic (ROC) analysis.Results: A total of 80 patients with LSS were included (35 men and 45 women; mean age 64.5 years), with 47 and 30 patients in the high and low groups, respectively. Multivariate logistic regression analysis revealed that the sagittal vertical axis (SVA; + 1; odds ratio [OR], 1.029; 95% confidence interval [CI], 1.005−1.052) and pelvic incidence (PI)-lumbar lordosis (LL; + 1; OR, 1.065; 95% CI 1.019–1.168) were significantly associated with LBP. ROC analysis revealed cut-off values of 47.0 mm and 30.5° of PI-LL, respectively.Conclusion: These cut-off values could have a high specificity and positive predictive value for LBP in patients with LSS due to spinopelvic malalignment. However, these cut-off values could also represent with a cause other than LSS due to spinopelvic malalignment.