bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem
Authors: Minnebo Yorick , De Paepe Kim , Raes Jeroen , de Wiele Tom Van 
Published: July 2021

ABSTRACT
A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research.
Leveraging histone modifications to improve genome annotations
Authors: Mendieta John Pablo , Marand Alexandre P , Ricci William A , Zhang Xuan , Schmitz Robert J 
Published: July 2021

Abstract
Accurate genome annotations are essential to modern biology; however, they remain challenging to produce. Variation in gene structure and expression across species, as well as within an organism, make correctly annotating genes arduous; an issue exacerbated by pitfalls in current in silico methods. These issues necessitate complementary approaches to add additional confidence and rectify potential misannotations. Integration of epigenomic data into genome annotation is one such approach. In this study, we utilized sets of histone modification data, which are precisely distributed at either gene bodies or promoters to evaluate the annotation of the Zea mays genome. We leveraged these data genome wide, allowing for identification of annotations discordant with empirical data. In total, 13,159 annotation discrepancies were found in Z. mays upon integrating data across three different tissues, which were corroborated using RNA-based approaches. Upon correction, genes were extended by an average of 2128 base pairs, and we identified 2529 novel genes. Application of this method to five additional plant genomes identified a series of misannotations, as well as identified novel genes, including 13,836 in Asparagus officinalis, 2724 in Setaria viridis, 2446 in Sorghum bicolor, 8631 in Glycine max, and 2585 in Phaseolous vulgaris. This study demonstrates that histone modification data can be leveraged to rapidly improve current genome annotations across diverse plant lineages.
Met is required for oligodendrocyte progenitor cell migration inDanio rerio
Authors: Ali Maria F , Latimer Andrew J , Wang Yinxue , Hogenmiller Leah , Fontenas Laura , Isabella Adam J , Moens Cecilia B , Yu Guoqiang , Kucenas Sarah 
Published: July 2021

AbstractDuring vertebrate central nervous system development, most oligodendrocyte progenitor cells (OPCs) are specified in the ventral spinal cord and must migrate throughout the neural tube until they become evenly distributed, occupying non-overlapping domains. While this process of developmental OPC migration is well characterized, the nature of the molecular mediators that govern it remain largely unknown. Here, using zebrafish as a model, we demonstrate that Met signaling is required for initial developmental migration of OPCs, and, using cell-specific knock-down of Met signaling, show that Met acts cell-autonomously in OPCs. Taken together, these findings demonstrate in vivo, the role of Met signaling in OPC migration and provide new insight into how OPC migration is regulated during development.
A Stress Test for German Nationalism: Protective Custody in Alsace-Lorraine during the First World War
Authors: Prott Volker 
Published: July 2021

Abstract
When the First World War broke out, the French government declared the return of Alsace-Lorraine its only public war aim, arguing that the population was ‘French in spirit’. In an age rife with claims of national self-determination, trapped in a protracted war of attrition and facing a nationally ambivalent population, the German state soon came under enormous pressure to ensure the loyalty and patriotism of the inhabitants of its western borderland. This article examines why Imperial Germany failed to meet this ‘stress test’. It focuses on the crucial but hitherto neglected issue of protective custody (Schutzhaft), whereby police and military authorities were able to arrest and detain ‘suspect’ civilians without charge or trial. The article finds that protective custody, an emergency measure under martial law, played a central role in the failure of German policy in Alsace-Lorraine: it undermined the rule of law, shifted the focus onto national dissent and gave rise to an atmosphere of suspicion and fear. The article also demonstrates that the Reichstag successfully put limits on protective custody in the second half of the war. Yet leaving the authoritarian doctrine of enforcing national loyalty in place, the more lenient administrative approach had a disintegrative rather than a stabilizing effect, preparing the ground for widespread disaffection with German rule months before the war ended.
Aging, Empathy, and Prosocial Behaviors During the COVID-19 Pandemic
Authors: Cho Isu , Daley Ryan T , Cunningham Tony J , Kensinger Elizabeth A , Gutchess Angela 
Published: July 2021

Abstract

Objectives
Previous literature suggests age-related increases in prosociality. Does such an age–prosociality relationship occur during the coronavirus disease 2019 (COVID-19) pandemic, or might the pandemic—as a stressor that may differently influence young and older adults—create a boundary condition on the relationship? If so, can empathy, a well-known prosocial disposition, explain the age–prosociality relationship? This study investigated these questions and whether the target (distant others compared to close others) of prosocial behaviors differs by age.

Methods
Participants completed a series of surveys on dispositional empathy and prosocial behaviors for a study assessing their experiences during the COVID-19 pandemic. There were 330 participants (aged 18–89) from the United States who completed all of the surveys included in the present analyses.

Results
Age was positively related to greater prosociality during the pandemic. Although empathy was positively associated with individuals’ prosociality, it did not account for the age–prosociality association. Interestingly, increasing age was associated with greater prosocial behaviors toward close others (i.e., family, friends).

Discussion
Results are discussed in the context of socioemotional goals and substantiate that findings of age differences in prosocial behaviors occur during the period of limited resources and threat associated with the COVID-19 pandemic.
Changes in health worker knowledge and motivation in the context of a quality improvement programme in Ethiopia
Authors: Quaife Matthew , Estafinos Abiy Seifu , Keraga Dorka Woldesenbet , Lohmann Julia , Hill Zelee , Kiflie Abiyou , Marchant Tanya , Borghi Josephine , Schellenberg Joanna 
Published: July 2021

Abstract
A knowledgeable and motivated workforce is critical for health systems to provide high-quality services. Many low- and middle-income countries face shortages in human resources and low health worker motivation but are also home to a burgeoning number of quality improvement (QI) programmes. This study evaluates whether and how motivation and clinical knowledge in three cadres of health workers changed in the context of a QI programme for maternal and newborn health in Ethiopia. This mixed-methods study used a pre–post comparison group design with matched comparison areas. We interviewed 395 health workers at baseline in April 2018 and 404 at endline in June 2019 from seven districts (woredas) with QI and seven comparison woredas. Three cadres were interviewed: health extension workers, facility-based skilled midlevel maternal and newborn care providers, and non-patient-facing staff. A qualitative component sought to triangulate and further elucidate quantitative findings using in-depth interviews with 22 health workers. Motivation was assessed quantitatively, exploratory factor analysis was used to categorize motivation dimensions, and regression-based difference-in-difference analyses were conducted. Knowledge was assessed through a clinical vignette. Qualitative data were analysed in a deductive process based on a framework derived from quantitative results. Although knowledge of the QI programme was high (79%) among participants from QI woreda at endline, participation in QI teams was lower (56%). There was strong evidence that health worker knowledge increased more in areas with QI than comparison areas. Three motivation dimensions emerged from the data: (1) ‘helping others’, (2) ‘pride and satisfaction’ and (3) ‘external recognition and support’. We found strong evidence that motivation across these factors improved in both QI and comparison areas, with weak evidence of greater increases in comparison areas. Qualitative data suggested the QI programme may have improved motivation by allowing staff to provide better care. This study suggests that although QI programmes can increase health worker knowledge, there may be little effect on motivation. Programme evaluations should measure a wide range of outcomes to fully understand their impact.
International stem cell tourism: a critical literature review and evidence-based recommendations
Authors: Lyons Samantha , Salgaonkar Shival , Flaherty Gerard T 
Published: July 2021

Abstract
Stem cell tourism is an emerging area of medical tourism activity. Frustrated by the slow translation of stem cell research into clinical practice, patients with debilitating conditions often seek therapeutic options that are not appropriately regulated. This review summarises recent developments in the field of stem cell tourism and provides clinicians with the information necessary to provide basic pretravel health advice to stem cell tourists. PubMed and Scopus databases were consulted for relevant publications, using combinations of the terms ‘stem cell’, ‘tourism’, ‘regenerative medicine’, ‘international’, ‘travel medicine’ and ‘environmental health’. The leading countries in the international stem cell tourism market are the USA, China, India, Thailand and Mexico. As the majority of clinics offering stem cell therapies are based in low- and-middle-income countries, stem cell tourists place themselves at risk of receiving an unproven treatment, coupled with the risk of travel-related illnesses. These clinics do not generally provide even basic travel health information on their websites. In addition to often being ineffective, stem cell therapies are associated with complications such as infection, rejection and tumorigenesis. Physicians, researchers, regulatory bodies, advocacy groups and medical educators are encouraged to work together to improve patient and physician education and address current legislative deficiencies.
A clinical andin vitroassessment of outpatient parenteral benzylpenicillin and ceftriaxone combination therapy for enterococcal endovascular infections
Authors: Ingram Paul R , Ng Jacinta , Mathieson Claire , Mowlaboccus Shakeel , Coombs Geoffrey , Raby Edward , Dyer John 
Published: July 2021

AbstractBackgroundAmoxicillin plus ceftriaxone combination therapy is now standard of care for enterococcal endocarditis. Due to amoxicillin instability in infusion devices, benzylpenicillin plus ceftriaxone may be substituted to facilitate outpatient parenteral antimicrobial therapy (OPAT) delivery, despite lack of guideline endorsement.ObjectivesTo assess the clinical efficacy of benzylpenicillin plus ceftriaxone for the management of enterococcal endovascular infections, in addition to assessing this combination’s in vitro synergy.Patients and methodsRetrospective cohort study assessing unplanned readmissions, relapses and mortality for 20 patients with endovascular Enterococcus faecalis infections treated with benzylpenicillin plus ceftriaxone delivered via OPAT. For a subset of isolates, synergism for both amoxicillin and benzylpenicillin in combination with ceftriaxone was calculated using a chequerboard method.ResultsPatients had endovascular infections of native cardiac valves (n = 11), mechanical or bioprosthetic cardiac valves (n = 7), pacemaker leads (n = 1) or left ventricular assistant devices (n = 1). The median duration of OPAT was 22 days, and the most frequent antimicrobial regimen was benzylpenicillin 14 g/day via continuous infusion and ceftriaxone 4 g once daily via short infusion. Rates of unplanned readmissions were high (30%), although rates of relapsed bacteraemia (5%) and 1 year mortality (15%) were comparable to the published literature. Benzylpenicillin less frequently displayed a synergistic interaction with ceftriaxone when compared with amoxicillin (3 versus 4 out of 6 isolates).ConclusionsLower rates of synergistic antimicrobial interaction and a significant proportion of unplanned readmissions suggest clinicians should exercise caution when treating enterococcal endovascular infection utilizing a combination of benzylpenicillin and ceftriaxone via OPAT.
Development and Validation of a Quantitative UHPLC–MS-MS Method for the Determination of Alpha-Chloralose in Feline Blood and Application on Blood Samples Collected from Cats with Symptoms of Alpha-Chloralose Poisoning
Authors: Windahl Ulrika , Lundgren Sandra , Sprycha Margareta , Tegner Cecilia , Dreimanis Kristoffer , Tevell Åberg Annica 
Published: July 2021

Abstract
Alpha-chloralose (AC) is used as a rodenticide as well as an anesthetic agent in laboratory animals. It was previously also used as an avicide. Detection of AC in blood samples or in body tissues collected postmortem is key for the diagnosis of clinical cases and a requirement for surveillance of secondary toxicosis, including potential cases in wild animals. Reports on poisoning of humans and non-laboratory animals confirmed by the detection of AC or its metabolites are available, however poisoning of domestic animals are rarely available. Furthermore, reports on clinical cases in domestic animals rarely report quantifications of AC in blood or body tissues. The present study describes the validation of a quantitative ultra high performance liquid chromatography--tandem mass spectrometry (UHPLC--MS-MS) method that can be used in cases of suspected AC poisoning in cats. The validation study showed the method to be fit for purpose. In serum, the limit of quantification was 100 ng/mL and the limit of detection was 30 ng/mL. The new analytical method was applied on blood samples collected from 20 individual cats with a preliminary clinical diagnosis of acute AC poisoning. AC was confirmed in all 20 feline blood samples, and the concentration range of AC was 538–17,500 ng/mL. The quantitative method developed in this study was found to be a fast and selective method for confirmation of AC poisoning using blood samples from cats.
Major Burn Injury Successfully Treated With Cultured Epithelial Autografts, a Case Series Presentation: Establishing Standard Clinical Practices
Authors: McGovern Kevin P , Rizzo Julie A 
Published: July 2021

Abstract
Cultured epithelial autografts have been an option for coverage of large surface area burns for over two decades. However, there remains extreme variability in clinical practice in wound bed preparation, application of cultured epithelial autografts, and postoperative wound care and rehabilitation practices, demonstrating the need for a standardized and multidisciplinary approach in the treatment of critically injured patients treated with cultured epithelial autografts. The purpose of this case series was to share the development of a clinical practice guideline and competency checklist in our institution where cultured epithelial autograft case volume is low. In this case series, we examined the medical records of three patients treated with cultured epithelial autografts at a single burn center over a period from 2015 to 2018. Operating room times and fluid resuscitation volumes were examined on days when cultured epithelial autograft grafting was performed. In order to facilitate meticulous postoperative wound care in a facility where only one to two cultured epithelial autograft applications are performed per year, a clinical practice guideline and competency checklist were generated and trialed on a series of nurses and rehabilitation therapists for the three applications of cultured epithelial autografts. Among the patients treated with cultured epithelial autografts, the average TBSA burned was 71.6%. Less intraoperative crystalloid administration and faster operative case times were associated with improved cultured epithelial autograft success. The inclusion of the clinical practice guideline and checklist into our practice led to reported improved confidence in patient care, along with the successful outcomes of these cultured epithelial autograft applications.
Molecular Phylogenetics and Population Genetics of the Dengue VectorAedes aegyptiFrom the Arabian Peninsula
Authors: Khater Emad I M , Baig Farrukh , Kamal Hany A , Powell Jeffery R , Saleh Amgad A 
Published: July 2021

AbstractAedes aegypti mosquito is the principal dengue vector in the Kingdom of Saudi Arabia (KSA); however, no study has addressed its ecology and population structure yet. Therefore, we report on Ae. aegypti phylo- and population genetics using three DNA markers: COI, ND4, and rDNA-ITS2. Sampling the immature stages of Ae. aegypti revealed that water storage tanks (34.3% of habitats) were the most productive and contained 33% of immatures stages. Other important habitats included containers for wastewater drainage (including air-conditioning and water cooler trays) and containers associated with ornamentation. Shallow water leakage spots (2.7% of habitats, 8% of immatures) can be considered rare-but-epidemiologically-important containers. Neighbor-joining (NJ) phylogenetic analysis of Ae. aegypti identified 8, 14, and 9 haplotypes of COI, ND4, and ITS2, respectively, and revealed high levels of genetic variation in Ae. aegypti populations of KSA. Global distribution of haplotypes also indicated multiple gene introductions into these populations, with high levels of intra-population genetic variation and continuous gene exchange. The neutrality values indicated a deficiency of alleles and suggested that the KSA Ae. aegypti loci tested did not follow a neutral model of molecular evolution. Fst values and AMOVA indicated that most of the genetic variation in the KSA Ae. aegypti populations is due to intra- rather than inter-population differences. This is the first comprehensive report on the phylo- and population genetics of Ae. aegypti from the Arabian Peninsula. This information expands our understanding of the ecology and population dynamics of this important arboviral vector for informed control efforts.
GRB host galaxies with strong H2 absorption: CO-dark molecular gas at the peak of cosmic star formation
Authors: Heintz K E , Björnsson G , Neeleman M , Christensen L , Fynbo J P U , Jakobsson P , Krogager J-K , Laskar T , Ledoux C , Magdis G , Møller P , Noterdaeme P , Schady P , de Ugarte Postigo A , Valentino F , Watson D 
Published: July 2021

ABSTRACT
We present a pilot search of CO emission in three H2-absorbing, long-duration gamma-ray burst (GRB) host galaxies at z ∼ 2–3. We used the Atacama Large Millimeter/submillimeter Array (ALMA) to target the CO(3 − 2) emission line and report non-detections for all three hosts. These are used to place limits on the host molecular gas masses, assuming a metallicity-dependent CO-to-H2 conversion factor (αCO). We find, $M_{\rm mol} \lt 3.5\times 10^{10}\, M_{\odot }$ (GRB 080607), $M_{\rm mol} \lt 4.7\times 10^{11}\, M_{\odot }$ (GRB 120815A), and $M_{\rm mol} \lt 8.9\times 10^{11}\, M_{\odot }$ (GRB 181020A). The high limits on the molecular gas mass for the latter two cases are a consequence of their low stellar masses M⋆ ($M_\star \lesssim 10^{8}\, M_{\odot }$) and low gas-phase metallicities ($Z\sim 0.03\, Z_{\odot }$). The limit on the Mmol/M⋆ ratio derived for GRB 080607, however, is consistent with the average population of star-forming galaxies at similar redshifts and stellar masses. We discuss the broader implications for a metallicity-dependent CO-to-H2 conversion factor and demonstrate that the canonical Galactic αCO will severely underestimate the actual molecular gas mass for all galaxies at z &gt; 1 with $M_\star \lt 10^{10}\, M_\odot$. To better quantify this we develop a simple approach to estimate the relevant αCO factor based only on the redshift and stellar mass of individual galaxies. The elevated conversion factors will make these galaxies appear CO-‘dark’ and difficult to detect in emission, as is the case for the majority of GRB hosts. GRB spectroscopy thus offers a complementary approach to identify low-metallicity, star-forming galaxies with abundant molecular gas reservoirs at high redshifts that are otherwise missed by current ALMA surveys.
Broad-band X-ray observations of the 2018 outburst of the changing-look active galactic nucleus NGC 1566
Authors: Jana Arghajit , Kumari Neeraj , Nandi Prantik , Naik Sachindra , Chatterjee Arka , Jaisawal Gaurava K , Hayasaki Kimitake , Ricci Claudio 
Published: July 2021

ABSTRACT
We study the nature of the changing-look active galactic nucleus NGC 1566 during its 2018 June outburst. During the outburst, the X-ray intensity of the source rises up to ∼25–30 times compared to its quiescent state intensity. We perform timing and spectral analysis of the source during pre-outburst, outburst, and post-outburst epochs using semisimultaneous observations with the XMM–Newton, Nuclear Spectroscopic Telescope Array(NuSTAR), and Neil Gehrels Swift Observatories. We calculate variance, normalized variance, and fractional rms amplitude in different energy bands to study the variability. The broad-band 0.5–70 keV spectra are fitted with phenomenological models, as well as physical models. A strong soft X-ray excess is detected in the spectra during the outburst. The soft-excess emission is found to be complex and could originate in the warm Comptonizing region in the inner accretion disc. We find that the increase in the accretion rate is responsible for the sudden rise in luminosity. This is supported by the ‘q’-shape of the hardness-intensity diagram that is generally found in outbursting black hole X-ray binaries. From our analysis, we find that NGC 1566 most likely harbours a low-spinning black hole with the spin parameter a* ∼ 0.2. We also discuss a scenario where the central core of NGC 1566 could be a merging supermassive black hole.