bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterogeneity of Staphylococcus epidermidis in prosthetic joint infections: time to reevaluate microbiological criteria?
Authors: Widerström Micael , Stegger Marc , Johansson Anders , Gurram Bharat Kumar , Larsen Anders Rhod , Wallinder Lars , Edebro Helen , Monsen Tor 
Published: October 2021

Abstract
Prosthetic joint infection (PJI) is a feared and challenging to diagnose complication after arthroplasty, with Staphylococcus epidermidis as the major pathogen. One important criteria to define PJI is the detection of phenotypically indistinguishable microorganisms with identical antibiotic susceptibility pattern in at least two different samples. However, owing to phenotypical variation within genetic clones and clonal variation within a phenotype, the criteria may be ambiguous. We investigated the extent of diversity among coagulase-negative staphylococci (CoNS) in PJI and characterised S. epidermidis isolates from PJI samples, specifically multiple S. epidermidis isolates identified in individual PJI patients. We performed a retrospective cohort study on 62 consecutive patients with PJI caused by CoNS from two hospitals in Northern Sweden. In 16/62 (26%) PJIs, multiple S. epidermidis isolates were available for whole-genome analyses. Hospital-adapted multidrug-resistant genetic clones of S. epidermidis were identified in samples from 40/62 (65%) of the patients using a combination of pulsed-field gel electrophoresis and multilocus sequence typing. Whole-genome sequencing showed the presence of multiple sequence types (STs) in 7/16 (44%) PJIs where multiple S. epidermidis isolates were available. Within-patient phenotypical variation in the antibiotic susceptibility and/or whole-genome antibiotic resistance gene content was frequent (11/16, 69%) among isolates with the same ST. The results highlight the ambiguity of S. epidermidis phenotypic characterisation as a diagnostic method in PJI and call for larger systematic studies for determining the frequency of CoNS diversity in PJIs, the implications of such diversity for microbiological diagnostics, and the therapeutic outcomes in patients.
The immunohistochemical expression of SSTR2A is an independent prognostic factor in meningioma
Authors: Fodi Christina , Skardelly Marco , Hempel Johann-Martin , Hoffmann Elgin , Castaneda Salvador , Tabatabai Ghazaleh , Honegger Jürgen , Tatagiba Marcos , Schittenhelm Jens , Behling Felix 
Published: October 2021

AbstractThe expression of somatostatin receptors in meningioma is well established. First, suggestions of a prognostic impact of SSTRs in meningioma have been made. However, the knowledge is based on few investigations in small cohorts. We recently analyzed the expression of all five known SSTRs in a large cohort of over 700 meningiomas and demonstrated significant correlations with WHO tumor grade and other clinical characteristics. We therefore expanded our dataset and additionally collected information about radiographic tumor recurrence and progression as well as clinically relevant factors (gender, age, extent of resection, WHO grade, tumor location, adjuvant radiotherapy, neurofibromatosis type 2, primary/recurrent tumor) for a comprehensive prognostic multivariate analysis (n = 666). The immunohistochemical expression scores of SSTR1, 2A, 3, 4, and 5 were scored using an intensity distribution score ranging from 0 to 12. For recurrence-free progression analysis, a cutoff at an intensity distribution score of 6 was used. Univariate analysis demonstrated a higher rate of tumor recurrence for increased expression scores for SSTR2A, SSTR3, and SSTR4 (p = 0.0312, p = 0.0351, and p = 0.0390, respectively), while high expression levels of SSTR1 showed less frequent tumor recurrences (p = 0.0012). In the Kaplan–Meier analysis, a higher intensity distribution score showed a favorable prognosis for SSTR1 (p = 0.0158) and an unfavorable prognosis for SSTR2A (0.0143). The negative prognostic impact of higher SSTR2A expression remained a significant factor in the multivariate analysis (RR 1.69, p = 0.0060). We conclude that the expression of SSTR2A has an independent prognostic value regarding meningioma recurrence.
Probabilistic modelling and verification using RoboChart and PRISM
Authors: Ye Kangfeng , Cavalcanti Ana , Foster Simon , Miyazawa Alvaro , Woodcock Jim 
Published: October 2021

AbstractRoboChart is a timed domain-specific language for robotics, distinctive in its support for automated verification by model checking and theorem proving. Since uncertainty is an essential part of robotic systems, we present here an extension to RoboChart to model uncertainty using probabilism. The extension enriches RoboChart state machines with probability through a new construct: probabilistic junctions as the source of transitions with a probability value. RoboChart has an accompanying tool, called RoboTool, for modelling and verification of functional and real-time behaviour. We present here also an automatic technique, implemented in RoboTool, to transform a RoboChart model into a PRISM model for verification. We have extended the property language of RoboTool so that probabilistic properties expressed in temporal logic can be written using controlled natural language.
Use of non-lethal endpoints to establish water quality requirements and optima of the endangered Topeka shiner (Notropis topeka)
Authors: Mott Rory T. , Rosenberger Amanda E. , Novinger Doug 
Published: October 2021

AbstractWater quality standards based on sub-lethal effects and performance optima for aquatic organisms, rather than onset of mortality, are more ecologically relevant for management of species of conservation concern. We investigated the effects of hypoxia, temperature (with acclimation), nitrogenous chemical compounds, and chloride on Topeka shiners (Notropis topeka) by monitoring behavioral responses to a reduction in oxygen and, using swimming speed, determining thermal optima and onset of effect for concentrations of nitrogenous compounds and chloride. We found ASR50 (i.e., dissolved oxygen concentrations where 50% of fish use aquatic surface respiration) to be 1.65 mg/L and ASR90 to be 1.08 mg/L of dissolved oxygen. Optimum temperatures for the species ranged from 17.7 to 28.0 °C, while predicted 100% mortality ranged from 33.7 to 40.3 °C, depending on the temperature at which fish were acclimated prior to experiments. Ammonia and sodium chloride reduced swimming speed at concentrations below known LC50 values, while nitrite concentrations did not correspond with swimming speed, but rather, post-experiment mortality. This provides insight into where Topeka shiners can not only persist, but also thrive. Although swimming speed may not be a suitable metric for determining the effects of all contaminants, our focus on optima and sub-lethal effects over tolerance allows selections of the most suitable reintroduction site matching the species’ physiological profile.
Balanced sampling of boxes from batches for assessing quality of fruits and vegetables in EU countries
Authors: Franceschi Sara , Betti Gianni , Fattorini Lorenzo , Gagliardi Francesca , Montrone Gianni 
Published: October 2021

AbstractThe best evaluation for the proportion of defective units in a batch of fruits and vegetables can be achieved by an exhaustive checking of all the boxes in the batch, that is prohibitive to perform in most cases. Usually, only a sample of boxes is checked. In EU countries, EU regulations establish to estimate the proportion of defective units in a batch by the proportion of defective units in the sample, without giving any rule for selecting boxes. Therefore, results are highly dependent on the subjective choice of boxes. In the present study, an objective design-based approach is considered to select boxes from batches, adopting balanced spatial schemes with equal inclusion probabilities. The schemes are able to select samples of boxes evenly spread throughout the batch also ensuring good statistical properties for the proportion of defective units in the sample as estimator of the proportion of defective units in the batch. The performance of these strategies is evaluated by means of a simulation study performed on real and artificial batches of apples, peppers and strawberries. A case study is considered to estimate the proportion of defective units in a batch of courgettes stored in a distribution center of a supermarket chain in Central Italy.
Metabolic profiles identify circulating biomarkers associated with heart failure in young single ventricle patients
Authors: O’Connell Thomas M. , Logsdon David L. , Mitscher Gloria , Payne R. Mark 
Published: October 2021

Abstract
Background
Children and young adults with single ventricle (SV) heart disease frequently develop heart failure (HF) that is intractable and difficult to treat. Our understanding of the molecular and biochemical reasons underlying this is imperfect. Thus, there is an urgent need for biomarkers that predict outcome and provide a rational basis for treatment, and advance our understanding of the basis of HF.

Objective
We sought to determine if a metabolomic approach would provide biochemical signatures of HF in SV children and young adults. If significant, these analytes might serve as biomarkers to predict outcome and inform on the biological mechanism(s) of HF.

Methods
We applied a multi-platform metabolomics approach composed of mass spectrometry (MS) and nuclear magnetic resonance (NMR) which yielded 495 and 26 metabolite measurements respectively. The plasma samples came from a cross-sectional set of young SV subjects, ages 2–19 years with ten control (Con) subjects and 16 SV subjects. Of the SV subjects, nine were diagnosed as congestive HF (SVHF), and 7 were not in HF. Metabolomic data were correlated with clinical status to determine if there was a signature associated with HF.

Results
There were no differences in age, height, weight or sex between the 3 cohorts. However, statistical analysis of the metabolomic profiles using ANOVA revealed 44 metabolites with significant differences between cohorts including 41 profiled by MS and 3 by NMR. These metabolites included acylcarnitines, amino acids, and bile acids, which distinguished Con from all SV subjects. Furthermore, metabolite profiles could distinguish between SV and SVHF subjects.

Conclusion
These are the first data to demonstrate a clear metabolomic signature associated with HF in children and young adults with SV. Larger studies are warranted to determine if these findings are predictive of progression to HF in time to provide intervention.
The circular economy and the Green Jobs creation
Authors: Sulich Adam , Sołoducho-Pelc Letycja 
Published: October 2021

AbstractThe circular economy (CE) is a proposal for a new, more sustainable, and durable economy model. As a consequence, this pro-environmental economic model induces visible changes in the labor market which are Green Jobs (GJs). This paper is focused on the creation of Green Jobs in the CE. The GJs are most visible in the environmental goods and services sector (EGSS). This study aims to investigate EGSS among 28 European Union countries in the years 2009–2019. The adopted method was literature research complemented by the statistical analysis of secondary data from Eurostat in the linear regression method. Then, some Sustainable Development Goals (SDGs) and their measure were used as main indicators reflecting changes in the labor market. Results are presented as a model indicating which of the SDGs can support CE and enhance a number of the Green Jobs. Presented results contribute to the science because combine factors influencing GJs creation in EGSS, in a CE perspective. This study underlines a lack of uniform methods for measuring and forecasting the effects of Green Jobs creation and indicates future research directions.
The Role of Plasma Exchange in the Treatment of Refractory Autoimmune Neurological Diseases: a Narrative Review
Authors: Jacob Saiju , Mazibrada Gordon , Irani Sarosh R , Jacob Anu , Yudina Anna 
Published: October 2021

AbstractAutoimmune neurological disorders are commonly treated with immunosuppressive therapy. In patients with refractory conditions, standard immunosuppression is often insufficient for complete recovery or to prevent relapses. These patients rely on other treatments to manage their disease. While treatment of refractory cases differs between diseases, intravenous immunoglobulin, plasma exchange (PLEX), and immune-modulating treatments are commonly used. In this review, we focus on five autoimmune neurological disorders that were the themes of the 2018 Midlands Neurological Society meeting on PLEX in refractory neurology: Autoimmune Encephalitis (AE), Multiple Sclerosis (MS), Neuromyelitis Optica Spectrum disorders (NMOSD), Chronic Inflammatory Demyelinating Polyradiculoneuropathy (CIDP) and Myasthenia Gravis (MG). The diagnosis of inflammatory neuropathies is often challenging, and while PLEX can be very effective in refractory autoimmune diseases, its ineffectiveness can be confounded by misdiagnosis. One example is POEMS syndrome (characterized by Polyneuropathy Organomegaly, Endocrinopathy, Myeloma protein, Skin changes), which is often wrongly diagnosed as CIDP; and while CIDP responds well to PLEX, POEMS does not. Accurate diagnosis is therefore essential. Success rates can also differ within ‘one’ disease: e.g. response rates to PLEX are considerably higher in refractory relapsing remitting MS compared to primary or secondary progressive MS. When sufficient efforts are made to correctly pinpoint the diagnosis along with the type and subtype of refractory autoimmune disease, PLEX and other immunotherapies can play a valuable role in the patient management.
Graphical abstract
The influence of job crafting on the quality of care provided by nursing home employees: The moderating effect of organizational identification
Authors: Romeo Marina , Yepes-Baldó Montserrat , Nordin Maria , Westerberg Kristina , Lundmark Robert , Morales Pamela 
Published: October 2021

AbstractOur goal was to analyze the positive effect of job crafting activities involving nursing home employees on their perceived quality of care, and the moderating effect of organizational identification. A two-wave non-experimental design (with an interval of 12 months) was used. The Job Crafting Questionnaire, the Identification-Commitment Inventory, and the Quality of Care Questionnaire (QoC) were administered to 226 nursing home employees in two waves. The results of the hierarchical regression analyses found significant association between job crafting subdimensions and quality of care twelve months later. Organizational identification was shown to play a moderating role in these relationships when analyzing the effect of cognitive crafting. In this sense, the effect of cognitive crafting on quality of care is only found with high levels of identification. The findings highlight the importance of the job crafting dimensions (task, relational and cognitive) when it comes to enhancing quality of care in residential homes for the elderly. This is especially relevant for cognitive crafting among employees with high levels of organizational identification. This research provides managers with guidance when allocating job crafting opportunities aimed at making improvements in quality of care. In this respect, organizations must offer job crafting training to stimulate and support their employees and, on the other hand, managers should encourage employees to craft their jobs, gearing their needs, abilities, and goals to corporate values and competencies.
A progressive development of a visual analysis interface of climate-related VGI
Authors: Navarra Carlo , Vrotsou Katerina , Opach Tomasz , Joling Almar , Wilk Julie , Neset Tina-Simone 
Published: October 2021

AbstractThis paper describes the progressive development of three approaches of successively increasing analytic functionality for visually exploring and analysing climate-related volunteered geographic information. The information is collected in the CitizenSensing project within which urban citizens voluntarily submit reports of site-specific extreme weather conditions, their impacts, and recommendations for best-practice adaptation measures. The work has pursued an iterative development process where the limitations of one approach have become the trigger for the subsequent ones. The proposed visual exploration approaches are: an initial map application providing a low-level data overview, a visual analysis prototype comprising three visual dashboards for more in-depth exploration, and a final custom-made visual analysis interface, the CitizenSensing Visual Analysis Interface (CS-VAI), which enables the flexible multifaceted exploration of the climate-related geographic information in focus. The approaches developed in this work are assessed with volunteered data collected in two of the CitizenSensing project’s campaigns held in the city of Norrköping, Sweden.
Statistical analysis of the best GIS interpolation method for bearing capacity estimation in An-Najaf City, Iraq
Authors: Al-Mamoori Sohaib Kareem , Al-Maliki Laheab A. , Al-Sulttani Ahmed Hashem , El-Tawil Khaled , Al-Ansari Nadhir 
Published: October 2021

AbstractThe presence of an economical solution to predict soil behaviour is essential for new construction areas. This paper aims to investigate the ultimate interpolation method for predicting the soil bearing capacity of An-Najaf city-Iraq based on field investigation information. Firstly, the engineering bearing capacity was calculated based on the in-site N-SPT values using dynamic loading for 464 boreholes with depths of 0–2 m, using the Meyerhof formula. The data then were classified and imported to the GIS program to apply the interpolation methods. Four deterministic and two geostatistical interpolation methods were applied to produce six bearing capacity maps. The statistical analyses were performed using two methods: the common cross-validation method by the coefficient of determination (R2) and root mean square error (RMSE), where the results showed that ordinary kriging (OK) is the ultimate method with the least RMSE and highest R2. These results were confusing so, the backward elimination regression (BER) procedure was applied to gain the definite result. The results of BER show that among all the deterministic methods, the IDW is the optimal and most significant interpolation method. The result of geostatistical methods shows that EBK is the best method in our case than the OK method. BER also applied to all six methods and shows that IDW is the ultimate significant method. The results indicate no general ultimate interpolation method for all cases and datasets type; therefore, the statistical analyses must be performed for each case and dataset.
Managing Water and Wetlands Based on the Tayal’s Interpretation of Utux and Gaga
Authors: Song Kuoyung Silan , LePage Ben , Fang Wei-Ta 
Published: October 2021

AbstractHumans first appeared on the planet about 3.5 million years ago and like most biota, they settled near wetlands because of the availability of food and water. The ancestors of our species understood and knew that water, wetlands, and healthy landscapes were essential for life. In Taiwan, the indigenous people have a long history being a part of and managing the natural resources, including wetlands in their respective habitats. Water and wetlands still play a substantial and significant role in the manner that the Tayal, an indigenous group of people in Taiwan use, preserve, care, protect, respect, and share the habitats and natural resources within which they live. The evolution of Taiwan’s tribal cultures, and probably most cultures on the planet are closely entwined with the resources present in each tribe’s habitat, especially water and wetlands. DNA results indicate 2 lineages of people migrated to Taiwan between 11,000 and 26,000 years ago and gave rise to 9 ethnic groups (Tajima et al. 2003). Today 16 indigenous cultures/tribes are recognized with each occupying different regions of Taiwan’s diverse landscape. Each tribe has its own language and culture and occupies its own geographic region, which contributes to Taiwan’s rich cultural history and diversity. The Tayal tribe is one of the larger tribes with about 88,000 people and the Smangus people are a subset of the Tayal tribe with a culture that is at least 6,000 years old. Culturally, the Tayal people consider themselves to be an element of the environment and their culture is defined by their relationship and interactions with the environment, including all of the other biotic, abiotic elements present in their habitats. In this paper we provide an overview of Tayal culture and philosophy, which determines how the Tayal people manage and protect their natural resources, especially water and wetlands following the tenets ofUtuxandGagathat comprise the entirety of their core cultural values. The cultural and language variations, nuances, environmental interpretations, and management techniques are specific to tribal groups and differences in geographic location and environmental settings.
Preparation and evaluation of novel functional fermented dairy products containing propolis and cinnamon
Authors: Gunes-Bayir Ayşe , Bilgin Mehmet Gültekin , Guclu Duygu , Pogda Sultan , Dadak Agnes 
Published: October 2021

AbstractNovel functional food products might be an easy accessible and eligible approach to help reduce the risk of severe viral infections including SARS-CoV-2. Hence a product containing probiotics, propolis and cinnamon was developed and interferences of the ingredients were characterized. Yogurts were prepared using starter cultures with propolis (0.03%) and cinnamon in various concentrations (0.3%, 1%, and 2.5%). Bifidobacterium animalis ssp. lactis, Lactobacillus acidophilus, Streptococcus thermophilus, and Lactobacillus delbrueckii subsp. bulgaricus were used as microorganisms for yogurt production. Chemical analysis revealed a decline of fat matter in the presence of propolis and/or cinnamon. Propolis had statistically significant suppressive effects on Bifidobacterium animalis ssp. lactis as well as on Lactobacillus delbrueckii subsp. bulgaricus numbers (p < 0.05). These effects were diminished in the presence of increasing cinnamon concentrations. For Lactobacillus acidophilus a statistically significant reducing effect on the number of colonies was observed in all products investigated. Nevertheless all samples met the standard of recommended level of ≥ 106 viable cells/g of a product. Propolis showed an inverse effect on Streptococcus thermophilus by increasing its colony numbers in yogurts. The probiotic yogurt samples containing propolis (0.03%) and cinnamon (2.5%) gained the highest number of points in the sensory evaluation compared to control.