bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse effects of pre-pregnancy maternal underweight on pregnancy and perinatal outcomes in a freeze-all policy
Authors: Tang Shengluan , Huang Jialyu , Lin Jiaying , Kuang Yanping 
Published: January 2021

Abstract
Background
Underweight and overweight may affect reproduction and interfere with treatment of infertility. In the present retrospective analysis, we sought to evaluate the effect of low body mass index (BMI) on pregnancy and perinatal outcomes in frozen–thawed embryo transfer (FET) cycles.

Methods
This study involved 8755 FET cycles in a single IVF center during the period from January 2009 to December 2018. Both pregnancy and perinatal outcomes were assessed in women who were underweight, normal weight, and overweight as defined based on a respective BMI < 18.5 kg/m2, ≥ 18.5 BMI < 24.9 kg/m2, and BMI ≥ 25 kg/m2.

Results
Being underweight was linked to reduced implantation rates as compared to a normal weight (33.56% vs. 37.26%). Similarly, when comparing outcomes in underweight women to those in normal weight women, rates of clinical pregnancy (48.14% vs. 53.85%) and ongoing pregnancy (43.04% vs. 50.47%) were reduced. Rates of miscarriage were markedly reduced in the normal weight group relative to the overweight group (10.73% vs. 13.37%). Perinatal outcomes were largely comparable for all groups, with the exception of very low birth weight rates (normal weight:0.58% vs. overweight: 2.03%), very small for gestational age rates (normal weight:1.31% vs. overweight:3.55%) and very preterm delivery rates (normal weight:0.82% vs. overweight: 2.03%), which were significantly elevated for overweight mothers.

Conclusions
These results indicate that being underweight is linked to negative pregnancy outcomes when undergoing FET-based IVF.
Counselling and psychotherapy service use in Chinese sexual minority populations: a nationwide survey
Authors: Wang Yuanyuan , Wilson Amanda , Hu Zhishan , Lu Li , Li Wengao , Peng Ke , Wu Lijuan , Xin Ying , Drescher Jack , Ou Jianjun , Chen Runsen 
Published: January 2021

Abstract
Background
This study investigated the prevalence and factors associated with counselling and psychotherapy service use among Chinese sexual minority populations.

Methods
A nationwide cross-sectional study was performed using snowball sampling method, which led to the inclusion of 18,193 participants. Participants’ sociodemographic background, clinical, and psychological data were gathered. Multivariate logistic regression analysis was performed to explore any associated factors.

Results
There were 2007 participants who had used counselling and psychotherapy service out of the total population. Among those who had used psychotherapy services, 80.2% participants perceived discrimination, 1.1% reported that they had been refused treatment by a counsellor and/or psychotherapist, 1.6% had experienced verbal harassment, and 8.4% reported that their counsellor and/or psychotherapist lacked knowledge and experience in treating sexual minorities. In addition, regression analyses indicated that those who were divorced/widowed, had religious beliefs, and those who had experienced discrimination, verbal harassment, and rejection for treatment by health professionals all had an increased likelihood of utilising counselling and psychotherapy service.

Conclusions
Service providers and policy makers in China should improve the quality and availability of counselling and psychotherapy services to address the mental health needs of sexual minority populations.
Antibiotic treatment in acute exacerbation of COPD: patient outcomes with amoxicillin vs. amoxicillin/clavulanic acid—data from 43,636 outpatients
Authors: Bagge Kristian , Sivapalan Pradeesh , Eklöf Josefin , Hertz Frederik Böetius , Andersen Christian Østergaard , Hansen Ejvind Frausing , Jarløv Jens Otto , Jensen Jens-Ulrik Stæhr 
Published: January 2021

Abstract
Background
For antibiotic treatment of Acute exacerbations of COPD (AECOPD) the National guidelines in Denmark recommend either first choice amoxicillin 750 mg TID (AMX) or amoxicillin with clavulanic acid 500 mg/125 mg TID (AMC). Addition of clavulanic acid offers a broader spectrum; opposite, AMX alone in a higher dose may offer more time above MIC. The aim of this study was to determine which of these regimens is associated with better outcome.

Methods
The Danish Registry of COPD (DrCOPD), a nationwide outpatient COPD registry, was crosslinked with medication data and hospital contacts. The first prescription of AMX or AMC after inclusion in DrCOPD was used as exposure variable. Adjusted Cox proportional hazards models were used to analyze the risk of hospitalization or death (combined) within 30 days and other endpoints.

Results
For the first treatment of AECOPD 12,915 received AMX, and 30,721 patients received AMC. AMX was associated with a decreased risk of pneumonia hospitalization or death (aHR 0.6, 95% CI 0.5–0.7; p < 0.0001) compared to AMC.

Conclusion
In AECOPD, empirically adding clavulanic acid to amoxicillin is not associated with a better outcome; it seems safe for these patients to be treated with amoxicillin alone.
Analysis of viral load in different specimen types and serum antibody levels of COVID-19 patients
Authors: Li Ling , Tan Chianru , Zeng Jia , Luo Chen , Hu Shi , Peng Yanke , Li Wenjuan , Xie Zhixiong , Ling Yueming , Zhang Xuejun , Deng E. , Xu Haixia , Wang Jue , Xie Yudi , Zhou Yaling , Zhang Wei , Guo Yong , Liu Zhong 
Published: January 2021

Abstract
Background
COVID-19 has caused a global pandemic and the death toll is increasing. However, there is no definitive information regarding the type of clinical specimens that is the best for SARS-CoV-2 detection, the antibody levels in patients with different duration of disease, and the relationship between antibody level and viral load.

Methods
Nasopharyngeal swabs, anal swabs, saliva, blood, and urine specimens were collected from patients with a course of disease ranging from 7 to 69 days. Viral load in different specimen types was measured using droplet digital PCR (ddPCR). Meanwhile, anti-nucleocapsid protein (anti-N) IgM and IgG antibodies and anti-spike protein receptor-binding domain (anti-S-RBD) IgG antibody in all serum samples were tested using ELISA.

Results
The positive detection rate in nasopharyngeal swab was the highest (54.05%), followed by anal swab (24.32%), and the positive detection rate in saliva, blood, and urine was 16.22%, 10.81%, and 5.41%, respectively. However, some patients with negative nasopharyngeal swabs had other specimens tested positive. There was no significant correlation between antibody level and days after symptoms onset or viral load.

Conclusions
Other specimens could be positive in patients with negative nasopharyngeal swabs, suggesting that for patients in the recovery period, specimens other than nasopharyngeal swabs should also be tested to avoid false negative results, and anal swabs are recommended. The antibody level had no correlation with days after symptoms onset or the viral load of nasopharyngeal swabs, suggesting that the antibody level may also be affected by other factors.
Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice
Authors: Miyata Shigeo , Kakizaki Toshikazu , Fujihara Kazuyuki , Obinata Hideru , Hirano Touko , Nakai Junichi , Tanaka Mika , Itohara Shigeyoshi , Watanabe Masahiko , Tanaka Kenji F. , Abe Manabu , Sakimura Kenji , Yanagawa Yuchio 
Published: January 2021

AbstractReduced expression of glutamate decarboxylase 67 (GAD67), encoded by the Gad1 gene, is a consistent finding in postmortem brains of patients with several psychiatric disorders, including schizophrenia, bipolar disorder and major depressive disorder. The dysfunction of GAD67 in the brain is implicated in the pathophysiology of these psychiatric disorders; however, the neurobiological consequences of GAD67 dysfunction in mature brains are not fully understood because the homozygous Gad1 knockout is lethal in newborn mice. We hypothesized that the tetracycline-controlled gene expression/suppression system could be applied to develop global GAD67 knockdown mice that would survive into adulthood. In addition, GAD67 knockdown mice would provide new insights into the neurobiological impact of GAD67 dysfunction. Here, we developed Gad1tTA/STOP−tetO biallelic knock-in mice using Gad1STOP−tetO and Gad1tTA knock-in mice, and compared them with Gad1+/+ mice. The expression level of GAD67 protein in brains of Gad1tTA/STOP−tetO mice treated with doxycycline (Dox) was decreased by approximately 90%. The GABA content was also decreased in the brains of Dox-treated Gad1tTA/STOP−tetO mice. In the open-field test, Dox-treated Gad1tTA/STOP−tetO mice exhibited hyper-locomotor activity and decreased duration spent in the center region. In addition, acoustic startle responses were impaired in Dox-treated Gad1tTA/STOP−tetO mice. These results suggest that global reduction in GAD67 elicits emotional abnormalities in mice. These GAD67 knockdown mice will be useful for elucidating the neurobiological mechanisms of emotional abnormalities, such as anxiety symptoms associated with psychiatric disorders.
The immunomodulatory effects of endocrine therapy in breast cancer
Authors: Huang Huanhuan , Zhou Jun , Chen Hailong , Li Jiaxin , Zhang Chao , Jiang Xia , Ni Chao 
Published: January 2021

AbstractEndocrine therapies with SERMs (selective estrogen receptor modulators) or SERDs (selective estrogen receptor downregulators) are standard therapies for patients with estrogen receptor (ER)-positive breast cancer. Multiple small molecule inhibitors targeting the PI3K-AKT-mTOR pathway or CDK4/6 have been developed to be used in combination with anti-estrogen drugs to overcome endocrine resistance. In addition to their direct antitumor effects, accumulating evidence has revealed the tumor immune microenvironment (TIM)-modulating effects of these therapeutic strategies, which have not been properly acknowledged previously. The immune microenvironment of breast tumors plays a crucial role in tumor development, metastasis and treatment response to endocrine therapy and immunotherapy. Therefore, in our current work, we comprehensively review the immunomodulatory effect of endocrine therapy and discuss its potential applications in combination with immune checkpoint inhibitors in breast cancer treatment.
Implementation of podiatry telephone appointments for people with rheumatic and musculoskeletal diseases
Authors: Palmer J. L. , Siddle H. J. , Redmond A. C. , Alcacer‐Pitarch B. 
Published: January 2021

AbstractBackgroundFoot health problems are common in the general population, and particularly so in people with rheumatic and musculoskeletal disorders (RMD). Several clinical guidelines state that people with RMDs should have access to foot health services, although service capacity is often limited. The current COVID‐19 pandemic has increased the need for alternative ways to provide patient care. The aim of this clinical audit was to review a newly implemented telephone follow‐up appointment service conducted within the Rheumatology Podiatry Department in Leeds, UK.MethodsFifty‐eight patients attending the Rheumatology Podiatry Department at Leeds Teaching Hospitals NHS Trust were contacted by telephone approximately 6–8 weeks following initial intervention. During the telephone consultation, all patients were asked pre‐defined questions relating to their symptoms, intervention efficacy, the need for further appointments and their preference for the type of consultation. To assess the cost of the telephone consultation the number of attempts needed in order to make successful contact, the duration of the call and the number of telephone follow‐up appointments completed in a working day were also recorded.ResultsTwenty‐five patients (43%) were successfully contacted within the 6–8 weeks stipulated time frame and were included in the analysis. Of the 25 contacted, twelve (48%) patients were successfully contacted on the first attempt. Ten (40%) were successfully contacted on the second attempt. The remaining three patients (12%) required 3 or more attempts to make successful contact. Telephone consultations were estimated not to last longer than 10 min, including notes screening and documentation. Eleven patients (44%) reported an improvement in their symptoms, thirteen (52%) reported no change and one patient (4%) reported their symptoms to be worse.ConclusionTelephone follow‐up consultations may be a potentially cost‐effective alternative to face‐to‐face appointments when implemented in a Rheumatology Podiatry Department, and provide an alternative way of providing care, especially when capacity for face‐to‐face contact is limited. The potential cost saving and efficiency benefits of this service are likely to be enhanced when telephone consultations are pre‐arranged with patients.
Long non-coding RNA FAM83H-AS1 acts as a potential oncogenic driver in human ovarian cancer
Authors: Yuan Xiaolei , Huang Ying , Guo Man , Hu Xiaowei , Li Peiling 
Published: January 2021

Abstract
Objective
Ovarian cancer (OC) is one of the most aggressive women cancers with increasing incidence and mortality rates worldwide. Long non-coding RNAs (lncRNAs) could as major players in OC process. Although FAM83H antisense RNA1 (FAM83H-AS1) is demonstrated play an important roles in a many cancers, the detailed function and mechanism has not been reported in OC.

Results
We integrated multiple kinds of bioinformatics approaches and experiments validated method to evaluate functions of FAM83H-AS1 in OC. Some differential expressed lncRNAs were identified between OC and normal control tissues. FAM83H-AS1 was one of most differentially expressed lncRNAs and up-regulated in multiple cancer types. Specially, expression of FAM83H-AS1 was higher in OC and showed difference in diverse stages. High FAM83H-AS1 expression is associated with worse pan-cancer and OC outcomes. FAM83H-AS1-centric network including lncRNA-miRNA, lncRNA-protein and lncRNA-mRNA ceRNA network were constructed to infer the function and mechanism of FAM83H-AS1. There were two methylation sites including cg01399317 and cg20519035 located at FAM83H-AS1. The methylation level of cg01399317 was correlated with gene expression of FAM83H-AS1. The expression level of FAM83H-AS1 was correlated with infiltration level of immune cell including macrophage, neutrphil and dendritic cell in OC patients. Lastly, qRT-PCR showed that the expression of FAM83H-AS1 was higher in OC tissues than normal control tissues.

Conclusion
Collectively, these results indicated that FAM83H-AS1 may act as an oncogenic driver and it may be a potential therapy target in OC.
Serum D-dimer level as a biomarker for identifying patients with isolated injury to prevent unnecessary whole-body computed tomography in blunt trauma care
Authors: Nakama Rakuhei , Yamamoto Ryo , Izawa Yoshimitsu , Tanimura Keiichi , Mato Takashi 
Published: January 2021

Abstract
Background
Unnecessary whole-body computed tomography (CT) may lead to excess radiation exposure. Serum D-dimer levels have been reported to correlate with injury severity. We examined the predictive value of serum D-dimer level for identifying patients with isolated injury that can be diagnosed with selected-region CT rather than whole-body CT.

Methods
This single-center retrospective cohort study included patients with blunt trauma (2014–2017). We included patients whose serum D-dimer levels were measured before they underwent whole-body CT. “Isolated” injury was defined as injury with Abbreviated Injury Scale (AIS) score ≤ 5 to any of five regions of interest or with AIS score ≤ 1 to other regions, as revealed by a CT scan. A receiver operating characteristic curve (ROC) was drawn for D-dimer levels corresponding to isolated injury; the area under the ROC (AUROC) was evaluated. Sensitivity, specificity, positive predictive value, and negative predictive value were calculated for several candidate cut-off values for serum D-dimer levels.

Results
Isolated injury was detected in 212 patients. AUROC was 0.861 (95% confidence interval [CI]: 0.815–0.907) for isolated injury prediction. Serum D-dimer level ≤ 2.5 μg/mL was an optimal cutoff value for predicting isolated injury with high specificity (100.0%) and positive predictive value (100.0%). Approximately 30% of patients had serum D-dimer levels below this cutoff value.

Conclusion
D-dimer level ≤ 2.5 μg/mL had high specificity and high positive predictive value in cases of isolated injury, which could be diagnosed with selected-region CT, reducing exposure to radiation associated with whole-body CT.
A rapid, parasite-dependent cellular response to Dirofilaria immitis in the Mongolian jird (Meriones unguiculatus)
Authors: Evans Christopher C. , Day Katherine M. , Chu Yi , Garner Bridget , Sakamoto Kaori , Moorhead Andrew R. 
Published: January 2021

AbstractBackgroundThe Mongolian jird (Meriones unguiculatus) has long been recognized as a permissive host for the filarial parasiteBrugia malayi; however, it is nonpermissive to another filarial parasite, canine heartworm (Dirofilaria immitis). By elucidating differences in the early response to infection, we sought to identify mechanisms involved in the species-specific clearance of these parasites. We hypothesized that the early clearance ofD. immitisin intraperitoneal infection of the jird is immune mediated and parasite species dependent.MethodsJird peritoneal exudate cells (PECs) were isolated and their attachment to parasite larvae assessed in vitro under various conditions:D. immitisandB. malayicultured separately, co-culture of both parasites, incubation before addition of cells, culture of heat-killed parasites, and culture with PECs isolated from jirds with matureB. malayiinfection. The cells attaching to larvae were identified by immunohistochemistry.ResultsIn vitro cell attachment to liveD. immitiswas high (mean = 99.6%) while much lower forB. malayi(mean = 5.56%). This species-specific attachment was also observed when both filarial species were co-cultured, with no significant change from controls (U(9, 14)= 58.5,p= 0.999). When we replicated these experiments with PECs derived from jirds subcutaneously infected withB. malayi, the results were similar (99.4% and 4.72% ofD. immitisandB. malayi, respectively, exhibited cell attachment). Heat-killing the parasites significantly reduced cell attachment toD. immitis(mean = 71.9%;U(11, 14)= 7.5,p< 0.001) while increasing attachment toB. malayi(mean = 16.7%;U(9, 15)= 20,p= 0.002). Cell attachment to both species was reduced when larvae were allowed a 24-h pre-incubation period prior to the addition of cells. The attaching cells were identified as macrophages by immunohistochemistry.ConclusionsThese results suggest a strongly species-dependent response from whichB. malayicould not confer protection by proxy in co-culture. The changes in cell attachment following heat-killing and pre-incubation suggest a role for excretory/secretory products in host immune evasion and/or antigenicity. The nature of this attachment is the subject of ongoing study and may provide insight into filarial host specificity.Graphical Abstract
Cytomegalovirus infection: friend or foe in rheumatoid arthritis?
Authors: Davignon Jean-Luc , Combe Bernard , Cantagrel Alain 
Published: January 2021

AbstractHuman cytomegalovirus (HCMV) is a β-herpesvirus that causes inflammation and remains for life in a latent state in their host. HCMV has been at the center of many hypotheses regarding RA.We have recently shown that HCMV infection impairs bone erosion through the induction of the mRNA-binding protein QKI5. Latently infected RA patients display a slower progression of bone erosion in patients from a national cohort. Our observations question the possible association between HCMV and the pathophysiology of RA. In this review, we examine the possibility that HCMV may be an aggravating factor of inflammation in RA while protecting from bone erosion. We also assess its relationship with other pathogens such as bacteria causing periodontitis and responsible for ACPA production.This review thus considers whether HCMV can be regarded as a friend or a foe in the pathogenesis and the course of RA.
Sleep quality and athletic performance according to chronotype
Authors: Lim Seung-Taek , Kim Do-Yoon , Kwon Hyeong-Tae , Lee Eunjae 
Published: January 2021

Abstract
Background
When studying the quality of sleep in relation to athletic performance, the athlete’s chronotype and habitual time consider important factors. We aim to investigate the sleep quality and athletes’ performance according to chronotype in elite athletes.

Methods
Three hundred forty elite athletes (males = 261, females = 79) were recruited for the present study. All participants were screening for chronotype by the Korean versions of the Morningness - Eveningness Questionnaire (MEQ-K). The Pittsburgh Sleep Quality Index (PSQI) and Wingate Anaerobic Test (WAnT) were measurement after screening.

Results
PSQI global score, PSQI sleep quality, PSQI sleep onset latency, PSQI sleep disturbance, and PSQI daytime dysfunction were significant differences among the groups. WAnT mean power (W), mean power (W/kg), peak power (W), and peak power (W/kg) were significant differences among the groups. A negative correlation coefficient was found between PSQI score and WAnT mean power (W) (r = − 0.256, p < 0.01), mean power (W/kg) (r = − 0.270, p < 0.01), peak power (W) (r = − 0.220, p < 0.01), and peak power (W/kg) (r = − 0.248, p < 0.01).

Conclusions
This study indicates that related poor sleep quality and late-type chronotype may reduce the athletes’ performance in elite athletes. In addition, the sleep quality is much higher in the early-type chronotype than in the late-type chronotype. Moreover, it also the athletic performance was better in the early-type chronotype than in the late-type chronotype.
Short tandem repeat near hypoxia response element (HRE) instead of HRE genetic variants in promoter calcitonin receptor-like receptor (CRLR) gene as risk factor in severe preeclampsia: a preliminary study
Authors: Herlambang  , Fitri Amelia Dwi , Syauqy Ahmad , Yuwono  , Puspasari Anggelia , Enis Rina Nofri , Faried Ahmad 
Published: January 2021

Abstract
Objective
Calcitonin receptor-like receptor (CRLR) regulates vasoconstriction and dilatation; the expression increases during hypoxia via activation of hypoxia response element (HRE) in CRLR gene promoter region. Variant in HRE, as well short tandem repeat (STR) variants near HRE in CRLR alters the gene expression. This study focused on a case–control study to investigate the expression of genetic typing CLRL promoter variant in pregnant women with severe preeclampsia and normal pregnancies, we also tried to describe interesting findings of the genetic expression in anemic patients in the severe preeclampsia group. Our aimed to observe the correlation of CRLR gene promoter variant and anemia in severe preeclampsia.

Results
There was no nucleotide variant in HRE; CACA box prior to HRE varied in length (15–24); CACA box with length > 20 was used as cut off point. Hb was lower in CACA box length ≥ 21 (10.33 ± 1.57) vs. < 21 (11.01 ± 1.67; p = 0.391). CACA box polymorphism and anemia were correlated in severe preeclampsia (p = 0.005) OR 0.038 (CI 0.003–0.544); not in normal (p = 0.069).