bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implikatur Percakapan dalam Film Orang Kaya Baru sebagai Bahan Ajar Teks Anekdot Kelas X
Authors: MaericE Widia Siska , Wijayawati Dyah , Nugroho Bivit Anggoro Prasetyo 
Published: June 2021

Penelitian ini berbentuk deskriptif kualitatif yang bertujuan untuk mendeskripsikan wujud pelanggaran prinsip kerja sama dan prinsip kesantunan dalam film Orang Kaya Baru, mendeskripsikan makna implikatur yang terkandung dalam film Orang Kaya Baru, dan mendeskripsikan implementasinya dalam pembelajaran teks anekdot. Metode pengumpulan data dalam penelitian ini menggunakan metode simak dengan teknik dasar sadap dan teknik lanjutannya teknik simak bebas libat cakap dan teknik catat. Pada tahap analisis data, peneliti menggunakan metode padan dengan teknik dasar pilah unsur penentu menggunakan daya pilah pragmatis, dan teknik lanjutan hubung banding memperbedakan. Adapun hasil analisis data disajikan dengan metode informal. Berdasarkan analisis data, terdapat pelanggaran pada setiap maksim dalam prinsip kerja sama dan prinsip kesantunan. Pelanggaran maksim-maksim tersebut menjadi penyebab munculnya implikatur percakapan dalam film Orang Kaya Baru. Adanya pelanggaran tersebut juga memberikan manfaat bagi pembaca, khususnya siswa. Melalui pemahaman tentang pelanggaran prinsip percakapan, diharapkan pembaca dapat menggunakan prinsip percakapan yang sesuai dalam berkomunikasi. Pemahaman mengenai implikatur dapat digunakan sebagai bahan ajar untuk mencapai tujuan pembelajaran pada Kompetensi Dasar (KD) 3.5 yaitu mengevaluasi teks anekdot dari aspek makna tersirat.
NILAI PENDIDIKAN KARAKTER DALAM NOVEL “SI ANAK SPESIAL” KARYA TERE LIYE: KAJIAN SOSIOLOGI SASTRA DAN RELEVANSINYA PADA PEMBELAJARAN BAHASA INDONESIA DI SMA
Authors: Karyoto Karyoto , Melasarianti Lalita , Martha Nia Ulfa 
Published: June 2021

Penelitian ini bertujuan untuk mendeskripsikan masalah sosial tokoh Burlian dan nilai pendidikan karakter yang terdapat pada novel Si Anak Spesial karya Tere Liye. Novel Si Anak Spesial ini menceritakan kisah tentang pentingnya sebuah pendidikan untuk masa depan seseorang. Tokoh yang menjadi pengantar pembentukan karakter dalam sebuah novel ini yaitu Mamak, Bapak, dan Pak Bin. Tokoh Mamak dan Bapak merupakan pembentuk karakter di lingkungan keluarga, sedangkan tokoh Pak Bin sebagai pendidik pembentukan karakter di lingkungan formal. Ketiga tokoh tersebutlah yang mengantarkan pendidikan karakter pada tokoh-tokoh yang ada dalam cerita, khususnya tokoh utama.
Kajian sosiologi sastra dan masalah sosial yang digunakan mengacu pada teorinya Faruk dan Soekanto, sedangkan untuk mengetahui nilai pendidikan karakter mengacu kepada kementrian pendidikan nasional tahun 2011. Penelitian ini merupakan penelitian deskriptif kualitatif. Data yang dikumpulkan berupa penggalan-penggalan cerita dalam novel kemudian dijelaskan melalui kata-kata. Metode yang digunakan ialah analisis isi, dengan teknik pengumpulan data, peneliti menggunakan teknik baca, simak, dan catat (BSC).
Berdasarkan penelitian, ditemukan 6 masalah sosial yaitu; masalah sosial kemiskinan, kejahatan, disorganisasi keluarga, pelanggaran terhadap norma-norma masyarakat, lingkungan hidup, dan birokrasi, dengan jumlah 36 data yang masuk masalah sosial. Sedangkan nilai pendidikan karakter yang ditemukan ada 13 nilai pendidikan karakter, yaitu; Nilai religius, jujur, disiplin, kerja keras, kreatif, rasa ingin tahu, cinta tanah air, menghargai prestasi, cinta damai, gemar membaca, peduli lingkungan, peduli sosial, dan tanggung jawab, dengan jumlah 40 data yang masuk nilai pendidikan karakter. Penelitian tersebut relevan dengan pembelajaran bahasa Indonesia di jenjang SMA kelas XII kurikulum 2013 berdasarkan KD 3.9 Menganalisi isi dan kebahasaan novel.
OPTIMALISASI PENGGUNAAN ALAT BANTU PEMBELAJARAN UNTUK MENINGKATKAN HASIL BELAJAR SERVIS ATAS
Authors: Sunardi Sunardi 
Published: June 2021

<p><em>The aim of the research is to improve the service learning outcomes of the volleyball through aids such as hula hoop, javelin, raffia cord, net, cone, plastic ball, sponsors ball in the learning process of students of SMA N 4 Surakarta.</em></p><p><em> </em><em>This research is a classroom action research (CAR). This research was conducted in two cycles which each cycle consists of planning, implementation of the action, observation,</em><em> </em><em>reflection. The subjects of the research are the students of</em><em> class</em><em> X</em><em> </em><em>IIS 4 SMA N 4 Surakart</em><em>a academic year 2016/2017, with the number of 30 students, 13 students and 17 students. Sources of data in this study are primary data and secondary data. Technique of collecting data with test and observation. Data validity using method triangulation. Data analysis using percentage technique, where from the data that has been obtained, then analyzed by percentage to see the learning result in volleyball game on learning in school.</em><em> </em><em></em></p><p><em>The results showed that learning with the optimization of the use of tools can improve the learning outcomes of service on volleyball in students of class X IIS 4 SMA N 4 Surakarta 2016/2017 academic year. In the first cycle, the results of service learning on the volleyball students reached 63.33% or 19 students of 30 students have entered the criteria thoroughly while 11 students or 36.67% in the category has not been completed. In the second cycle there is an increase in the percentage of student learning outcomes in the category of completion of 83.33% or a number of 25 students. Until the end of the meeting there are 5 students or 16.67% that have not been completed.</em></p><p><em>Based on the result of the research, it can be concluded that the learning with the optimization of the use of the tools can improve the learning outcomes of service on the volleyball in the students of class X IIS 4 SMA N 4 Surakarta in the academic year 2016/2017.</em></p><p><em> </em></p>
Liquid Biopsy as an Option for Predictive Testing and Prognosis in Patients with Lung Cancer
Authors: Qvick Alvida , Stenmark Bianca , Carlsson Jessica , Isaksson Johan , Karlsson Christina , Helenius Gisela 
Published: June 2021

Abstract
Background: The aim of this study was to investigate the clinical value of liquid biopsy as a primary source for variant analysis in lung cancer. In addition, we sought to characterize liquid biopsy variants and to correlate mutational load to clinical data.Methods: circulating cell-free DNA was extracted from plasma from patients with lung cancer (n=60) and controls with benign lung disease (n=16). Variant analysis was performed using the AVENIO ctDNA Surveillance kit and the results were correlated to clinical and variant analysis data from tumor tissue or cytology retrieved from clinical routine diagnostics. Results: There were significantly more variants detected in lung cancer cases compared to controls (p=0.011), but no difference between the histological subgroups of lung cancer was found (p=0.465). Furthermore, significantly more variants were detected in patients with stage IIIb-IV disease compared to patients with stage I-IIIa (median 7 vs 4, p=0.017). Plasma cfDNA mutational load was significantly associated with overall survival (p=0.010). The association persisted when adjusted for stage and ECOG performance status (HR: 3.64, 95% CI: 1.37-9.67, p=0.009). Agreement between tumor and plasma samples significantly differed with stage; patients with stage IIIb-IV disease showed agreement in 88.2% of the cases with clinically relevant variants, compared to zero cases in stage I-IIIa (p=0.004). Furthermore, one variant in EGFR, two in KRAS, and one in BRAF were detected in plasma but not in tumor samples. Conclusion: This study concludes that in the vast majority of advanced NSCLC patients a reliable variant analysis can be performed using liquid biopsy from plasma. Furthermore, we found that the number of variants in plasma is associated with prognosis, possibly indicating a strategy for closer follow up on this crucial patient group.
Mechanistic Interrogation of Cell Transformation in Vitro: The Transformics Assay as an Exemplar of Oncotransformation
Authors: Pillo Gelsomina , Mascolo Maria Grazia , Zanzi Cristina , Rotondo Francesca , Serra Stefania , Bortone Francesco , Grilli Sandro , Vaccari Monica , Jacobs Miriam Naomi , Colacci Annamaria 
Published: June 2021

Abstract
The rodent-based life-time cancer bioassay is still the basic experimental paradigm to assess the carcinogenic potential of chemicals, with relatively little progress regarding the acceptance of in vitro tests. The Transformics Assay is an in vitro test which combines the BALB/c 3T3 Cell Transformation Assay (CTA) with microarray transcriptomics. It has been shown to improve upon the mechanistic understanding of the CTA, helping to identify mechanisms of action leading to chemical-induced transformation thanks to RNA extractions in specific time points along the process of in vitro transformation. In this study the lowest transforming concentration of the carcinogenic benzo(a)pyrene (B(a)P) has been tested in order to find molecular signatures of initial events relevant for oncotransformation. Some biological interpretations are presented related to the Enrichment Analysis provided by Metacore. Results show that after 72 hrs of exposure, the immune and inflammatory responses are the main processes involved in cell response. Furthermore, pathways and processes related to cell cycle regulation, cytoskeletal adhesion and remodeling processes, cell differentiation and transformation were observed. All these data are interpreted as a consequence of the initial aryl hydrocarbon receptor (AhR) activation leading to a cascade of cellular events and microenvironment modification.
Improving Coarse Grain Models of Protein Folding through Weighting of Polar-polar/hydrophobic-hydrophobic Interactions into Crowded Spaces
Authors: Beltrán Hiram Isaac , Alas-Guardado Salomón J. , Perez Pedro Pablo Gonzalez 
Published: June 2021

Abstract
In this piece of work were tested 7 Hydrophobic-Polar sequences in two types of 2D-square space lattices, homogeneous and correlated, the latter simulating molecular crowding included as a geometric boundary restriction. The optimization of the 2D structures was carried out using a variant of Dill's model, inspired by the convex function, which takes into account both the hydrophobic (Dill’s model) and polar interactions, aimed to include more structural information to reach better folding solutions. While using correlated networks, the degrees of freedom in the folding of sequences were limited, and as a result in all cases more successful structural trials were found in comparison to the homogeneous lattice. In particular, the S5 sequence turned out to be the most difficult sequence of the seven folded, this perhaps due to the intrinsic i) degrees of freedom and ii) motifs of the expected 2D HP structure. Regarding S2 and S6 sequences, although optimal folding was not achieved for neither of the two approaches, folding with correlated network approach not only produced better results than homogeneous space, but for both sequences the best values found with crowding were very close to the expected optimal fitness. The sequences S1-S4 and S6 were better folded with medium lattice units for the correlated media, instead, S5 and S7 were better folded with a bit larger degree of lattice unit, revealing that depending on the degrees of freedom and particular folding motifs in each sequence would require particular crowding to achieve better folding. Finally, we claim that in all folded sequences in crowded spaces achieve better results than homogeneous ones.
Disparate Access To Breast Cancer Screening And Treatment
Authors: Castaldi Maria , Smiley Abbas , Kechejian Katharine , Butler Jonathan , Latifi Rifat 
Published: June 2021

Abstract
Background: Barriers to breast cancer screening remain despite Medicaid expansion for preventive screening tests and implementation of patient navigation programs (PNP) under the Affordable Care Act. Women from underserved communities experience disproportionately low rates of screening mammography. This study compares barriers to breast cancer screening among women at an inner-city safety-net center (City) and those at a suburban county medical center (County). Inner city and suburban county medical centers’ initiatives were studied to compare outcomes of breast cancer screening and factors that influence access to care.Methods: Women 40 years of age or older delinquent in breast cancer screening were offered PN services between October 2014 and September 2019. Four different screening time-to-event intervals were investigated: time from PN acceptance to screening mammography, to diagnostic mammography, to biopsy, and overall screening completion time. Barriers to complete breast cancer screening between the two centers were compared.Results: Women from lowest income quartiles took significantly longer to complete breast cancer screening when compared to women from higher income quartiles when a barrier was present, regardless of barrier type and center. Transportation was a major barrier to screening mammography completion, while fear was the major barrier to abnormal screening work up. Table 1.Conclusion: Disparity in breast cancer screening and management persists despite implementation of a PNP. In the presence of a barrier, women from the lowest income quartiles have prolonged breast cancer screening completion time regardless of center or barrier type. Women who experience fear have longest screening time completion. Future directions aim to increase resource allocation to ameliorate wait times in overburdened safety-net hospitals as well as advanced training for PN to alleviate women’s fears.
SERS on Bimetallic Nanostructured thin films
Authors: s revathy m , Murugesan D , Jayram Naidu Dhanpal 
Published: June 2021

Abstract
Thin films and Surface Enhanced Raman spectroscopy have a strong bonding towards development of Sensors. From last 4 decades SERS has been used as effective tool for detection of toxic dyes, in food industry and agriculture world. To minimize the cost and fabrication over large surface is the most challenging task in substrate fabrication. In the present work an attempt has been made towards dual coatings, which could act as an effective SERS Substrates. An effective and facile approach of low cost bi-metallic Nanostructured film has been fabricated using thermal evaporation. Using the standard characterization techniques such as FE-SEM and XRD, the obtained films were Rhodamine 6G was used as an analyte for the SERS studies. The detection of R6G was up to 10− 10mol l− 1solution.The present bi-metallic coating can be serves as an excellent SERS active surface and provides a versatile pathway to fabricate anisotropic nanostructure on a glass film.
The Relationship Between Mindfulness And Mental Health: A Mediating Model of Sense of Purpose In Life Among Graduate Students
Authors: Wang Yibin , Tian Tian , Wang Junjie 
Published: June 2021

Abstract
The purpose of the present study is to examine the relationship between mindfulness and mental health of graduate students and the mediating effects of sense of purpose in life on mindfulness and mental health.The participants include 419 graduate students from 6 universities in China.The Hayes Process is adopted to analyze the effects of the sense of purpose in life on mindfulness and mental health of graduate students.The results reveals that mindfulness can predict the mental health of graduate students positively and significantly.The sense of purpose in life is found to mediate the relationship between mindfulness and mental health.In further moderated mediation analyses,the effect of mindfulness on mental health can be adjusted by family economic condition.The type of degree can adjust the effect of mindfulness on sense of purpose,and professional interest can adjust the mediating effect of sense of purpose.Finally,this study discusses several empirical and methodological implications of the findings.
Reproductive Hormones Imbalance, Germ Cell Apoptosis, Abnormal Sperm Morphophenotypes and Ultrastructural Changes in Testis of African Giant Rats (Cricetomys gambianus, Waterhouse, 1840) Exposed to Sodium Metavanadate Intoxication
Authors: Usende Ifukibot Levi , Oyelowo Fatima Oyenike , Adikpe Agbonu Oluwa , Emikpe Benjamin Obukowho , Nafady Allam Abdel Hamid Mohamed , Olopade James Olukayode 
Published: June 2021

Abstract
Environmental exposure to vanadium has been on the increase in recent time. This metal is a known toxicant. The current study was conducted to investigate the reproductive toxicity of sodium metavanadate (SMV) in male African giant rats. Administration of SMV was done intraperitoneally daily for 14 consecutive days at a dosage of 3mg/kg body weight. Sterile water was administered to the control group. We analyzed serum reproductive hormones, sperm reserve and quality as well as testicular ultrastructural changes following SMV treatment. Our results showed SMV exposed AGR group had statistically increased progesterone but decreased testosterone, FSH and LH concentrations. Also, SMV treated group had statistically decreased sperm motility and mass activity with increased percentage of abnormal morphophenotypes of spermatozoa and upregulation of P53 immunopositive cell. Ultrastructural study revealed vocuolation of germ and Sertoli cells, cytoplasmic and nucleus; and mitochondrial swelling and vacuolations were also observed. There was severe disintegration of the seminiferous tubules, atrophy and degeneration of myeloid cells and apoptosis of the Leydig, Sertoli and germ cells. In conclusion, intraperitoneal SMV exposure exerts severe adverse effects on some serum reproductive hormones, reduction of sperm reserve and quality, apoptosis and degenerative changes of the Leydig, Sertoli and germ cells which can lead to infertility.
Public Perception of the COVID-19 Pandemic on Chinese Social Networking Service (Weibo): Sentiment Analysis and Fuzzy-C-Means Model
Authors: Han Feng , Zhang ZiHeng , Zhang HongJian , Aoki Tomohiko , Ogasawara Katsuhiko 
Published: June 2021

Abstract
BackgroundSocial media analysis tools have been used to monitor public sentiment and communication methods during public health emergencies.Public health emergencies are required to better understand the impact of the crisis on the public and to provide reference material for the prevention of future public health emergencies. We are concentrating on the sentiments around the public health emergency created by COVID-19.ObjectiveThis study aims to better understand the impact of public health emergencies on citizens and provide reference material for future public health emergency prevention.MethodsThe Fuzzy-c-means method was used to divide the 850,083 content of Weibo from January 24, 2020, to March 31, 2020, into seven categories of emotions: fear, happiness, disgust, surprise, sadness, anger, and good. The changes in emotion were tracked over time.ResultsThe results indicated that people showed "surprise" overall (55.89%); however with time, the "surprise" decreased. As the knowledge regarding the coronavirus disease 2019 (COVID-19) increased (contents about COVID-19 knowledge: from 21.16% to 4.19%), the "surprise" of the citizens decreased (from 59.95% to 46.58%). Citizens' feelings of "fear" and "good" increased as the number of deaths associated with COVID-19 increased ("fear”: from 15.42% to 20.95% "good”: 10.31% to 18.89%). As the infection was suppressed, the feelings of "fear" and "good" diminished ("fear”: from 20.95% to 15.79% "good”: from 18.89% to 8.46%).ConclusionsIn this study, the emotions and changes in emotions of Weibo users were analyzed in chronological order. The results of this study can prepare for future public health emergencies.
MiR-124 Promotes Microglial M2 Polarization Through TLR4/MyD88/NF-κB p65/NLRP3 Signaling In Palmitic Acid Treated-BV2 Cells
Authors: Yang Caixia , Sui Guanghong , Chen Zheng , Wang Feng 
Published: June 2021

Abstract
AimNeuroinflammation is an explanation why obesity or high-fat diet induce central nervous system disorders. MiR-124, as a highly expressed microRNA in brain, might alleviate neuroinflammation through regulating microglial M1/M2 polarization, but its mechanism is unclear. The aim of the study was to explore whether miR-124 exerted its effect mentioned above through TLR4/MyD88/NF-κB p65/NLRP3 signaling in palmitic acid treated-microglia.MethodsPrepared BV2 cells were treated with palmitic acid to establish an in vitro model of high-fat diet. MiR-124 mimic and inhibitor were adopted to up-regulate and down-regulate the expression of miR-124. TAK-242 and NLRP3 siRNA were used to down-regulate the expression of TLR4 and NLRP3. Expression of miR-124, signaling proteins (TLR4, MyD88, NF-κB p65), inflammasome markers (NLRP3, IL-1β) and microglial polarization markers (CD206, Arg-1, CD86, iNOS) was measured by qPCR and western blotting. Pyroptosis rate was assessed using flow cytometry.ResultsFirst, palmitic acid up-regulated the TLR4/MyD88/NF-κB p65 signaling, increased the NLRP3 expression, elevated the pyroptosis rate and inhibited the M2 polarization in BV2 cells. Second, miR-124 mimic and inhibitor separately alleviated and aggravated the effect of palmitic acid on microglial polarization and NLRP3 expression. MiR-124 mimic also down-regulated the TLR4/MyD88/NF-κB p65 signaling. Third, TAK-242 did not affect the expression of miR-124, but can simulate the protective effect of miR-124 mimic on microglial polarization and NLRP3 expression. Fourth, NLRP3 siRNA can also promoted M2 polarization in BV2 cells.ConclusionMiR-124 promoted microglial M2 polarization through TLR4/MyD88/NF-κB p65/NLRP3 signaling in palmitic acid treated-BV2 cells
Cinobufacini Injection Suppresses the Proliferation of Human Osteosarcoma Cells by Inhibiting PI3K/Akt Signaling Pathway
Authors: Chen Changbao , Zhai Yu , Chen Yuru , Yuan Ye , Hua Shengyu , Wang Yu 
Published: June 2021

Abstract
Background: Cinobufacini injection (CI), an aqueous extraction from the Cutis Bufonis, is broadly used in clinical treatment of cancer in China. However, the underlying molecular mechanisms of CI in treating osteosarcoma (OS) remain unclear. Aberrant activation of PI3K-AKT signaling pathway is the cause of many types of cancer, including OS. Therefore, we investigated the effect of CI on proliferation, apoptosis and cell cycle of OS cells and elucidated the molecular mechanism of CI in inhibiting OS cells. Methods: Cell proliferation of U2OS and MG63 cells after CI treatment was measured by CCK-8 assay, colony formation and morphological changes. Additionally, the cell cycle arrest and apoptosis induced by CI, were determined by FACS and Western blot analysis. The mechanisms of CI on OS were evaluated by RNA-seq and Western blot analysis. Results: We founf that CI reduced the proliferation of U2OS and MG63 cells in a dose- and time- dependent manner. Furthermore, CI induced the U2OS cells cycle arrest in G0/G1 phase, but the MG63 cells cycle arrest in G2/M phase. Consequently, CI triggered the apoptosis in both OS cells, with enhanced caspase-3 activity and decreased expression of Bcl-2/Bax. In addition, RNA-seq data indicated that PI3K-Akt signaling pathway played an essential role in CI treatment. Moreover PI3K and phosphorylation of AKT (p-AKT) were significantly down-regulated by CI in both OS cells. Conclusions: These results indicate that CI significantly inhibited the proliferation, induced the cell cycle arrest, as well as apoptosis in human OS cells, which is mediated by the inactivation of PI3K-Akt signaling pathway. These findings suggest that CI may have potential for the treatment of OS.