bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing food and nutrition literacy in children and adolescents: a systematic review of existing tools
Authors: Carroll Nicholas , Perreault Maude , Ma David WL , Haines Jess 
Published: November 2021

AbstractObjective:Food literacy (FL) and nutrition literacy (NL) are concepts that can help individuals to navigate the current food environment. Building these skills and knowledge at a young age is important for skill retention, confidence in food practices and supporting lifelong healthy eating habits. The objectives of this systematic review were to: (i) identify existing tools that measure FL and NL among children and/or adolescents and (ii) describe the psychometric properties.Design:A 4-phase protocol was used to systematically retrieve articles. The search was performed in May 2021. Study characteristics and psychometric properties were extracted, and a narrative synthesis was used to summarise findings. Risk of bias was assessed using the COSMIN checklist.Setting:Six databases were searched to identify current tools.Participants:Children (2–12 years) and adolescents (13–18 years) participated in this study.Results:Twelve tools were identified. Three tools measured FL, 1 tool measured NL, 4 tools measured both FL and NL, and 4 tools measured subareas of NL—more specifically, critical NL, food label and menu board literacy. Most tools were self-reported, developed based on a theoretical framework and assessed some components of validity and/or reliability for a specific age and ethnic group. The majority of tools targeted older children and adolescents (9–18 years of age), and one tool targeted preschoolers (3–6 years of age).Conclusions:Most widely used definitions of FL and NL do not acknowledge life-stage specific criterion. Continued efforts are needed to develop a comprehensive definition and framework of FL and NL appropriate for children, which will help inform future assessment tools.
Translating fathers’ support for breastfeeding into practice
Authors: Baldwin Sharin , Bick Debra , Spiro Alison 
Published: November 2021

Abstract
Breastfeeding has numerous health benefits for the mother and child. For breastfeeding to be successful and continue for longer, women need adequate support. Fathers/partners play an important role in providing this support to women, but research suggests that fathers/partners often feel inadequately informed and supported by health professionals. Midwives and health visitors are in ideal positions to offer women and their partner’s timely and relevant breastfeeding information and support throughout the perinatal period. This article discusses the benefits of breastfeeding, presents research evidence of the crucial role fathers/partners play in promoting and supporting breastfeeding, and recommends ways in which health professionals can provide breastfeeding information and support to fathers/partners.
Coethnicity Beyond Clientelism: Insights from an Experimental Study of Political Behavior in Lebanon
Authors: Cammett Melani , Kruszewska-Eduardo Dominika , Parreira Christiana , Atallah Sami 
Published: November 2021

AbstractA large literature finds that coethnicity primarily shapes voter behavior through material exchanges, particularly clientelism. Yet identity groups provide distinct psychological and social benefits that also compel people to vote based on coethnicity. Does coethnicity matter for vote choice, net of instrumental considerations? We address this question using a conjoint experiment in Lebanon, which asked a nationally representative sample of citizens to choose between potential candidates in national elections. We find that coethnicity is the single strongest predictor of electoral support, more important than party affiliation, provision of clientelism, or programmatic platform. Coethnicity does not significantly alter perceptions of candidates who provide clientelism, including high-value goods like patronage employment. Furthermore, citizens who feel closer to their ethnic group are more likely to vote on the basis of coethnicity, as are those with lower levels of trust in state institutions. Collectively, these findings suggest that coethnic voting in diverse polities is not driven solely by clientelism, but also by less immediately material concerns about security and belonging.
Dynamic Hillslope Soil Moisture in a Mediterranean Montane Watershed
Authors: Dymond Salli F. , Wagenbrenner Joseph W. , Keppeler Elizabeth T. , Bladon Kevin D. 
Published: November 2021

AbstractVariations in hillslope soil moisture control forest hydrologic fluxes and storage pools, yet sparse observations combined with the complexity and heterogeneity of water movement and storage in the vadose zone can make temporal and spatial patterns and processes difficult to predict. We used two years of field observations of volumetric soil moisture at three depths (15, 30, and 100 cm) across five topographic positions (riparian, toeslope, sideslope, shoulder, and ridge) along three hillslope transects to better understand how soil moisture changes with hillslope position and through time. As expected, we found higher values of soil moisture at all depths at the riparian and toeslope positions. Unexpectedly, we found that ridges were particularly wet during the wet winter months and dried quickly during the summer months, indicating that topography alone cannot account for mean wet season soil moisture in our Mediterranean climate field site. The variability in soil moisture across all soil depths and topographic positions was greatest when soils were dry and decreased under wet soil conditions; this variability remained high in the deeper soil horizons, regardless of season. Lastly, event analysis suggests that the response to early season rainfall was highly variable along the hillslopes and was likely dominated by localized controls such as microtopography and vegetation as well as soil texture, antecedent moisture conditions, and rainfall characteristics. Our results suggest that the drivers of wet and dry season soil moisture dynamics can vary across topographic positions along a hillslope and do not always follow topographic controls.
Inferring the Horizontal Speed of an Ionospheric Irregularity From a Single GPS Scintillation Receiver at High Latitudes
Authors: Hong Junseok , Chung Jong‐Kyun , Ham Young‐Bae , Kwak Young‐Sil , Kim Yong Ha 
Published: November 2021

AbstractWe propose a new technique to infer the horizontal speed of ionospheric irregularity at high latitudes from the refractive components of the global positioning system (GPS) L1 (1575.42 MHz) and L2 (1227.60 MHz) carrier phases using only a single ground‐based receiver that can log measurements at 50–100 Hz. The test data at Jang Bogo Station (geographic: 74.6°S and 164.2°E; geomagnetic: 77.2°S) in Antarctica and at Sachs Harbor (geographic: 71.9°N and 234.7°E; geomagnetic: 75.6°N) in the Arctic were analyzed for a one‐year period in 2019. The results were compared with data from ground‐based radar observations, the Vertical Incidence Pulsed Ionospheric Radar (VIPIR) in Antarctica and the Canadian Advanced Digital Ionosonde (CADI) in the Arctic, which measures the ion drift velocity at the apex. Although it is difficult to directly compare GPS and radar measurements due to the steep gradient of fast plasma motion in narrow regions at high latitudes, the probability density of the ionospheric irregularity speeds from the two different instruments were consistent with the correlation coefficients of 0.81 and 0.85 in the Southern and Northern hemispheres.
The acute effect of fasted exercise on energy intake, energy expenditure, subjective hunger and gastrointestinal hormone release compared to fed exercise in healthy individuals: a systematic review and network meta-analysis
Authors: Frampton James , Edinburgh Robert M. , Ogden Henry B. , Gonzalez Javier T. , Chambers Edward S. 
Published: November 2021

Abstract
Objective
To determine the acute effect of fasted and fed exercise on energy intake, energy expenditure, subjective hunger and gastrointestinal hormone release.

Methods
CENTRAL, Embase, MEDLINE, PsycInfo, PubMed, Scopus and Web of Science databases were searched to identify randomised, crossover studies in healthy individuals that compared the following interventions: (i) fasted exercise with a standardised post-exercise meal [FastEx + Meal], (ii) fasted exercise without a standardised post-exercise meal [FastEx + NoMeal], (iii) fed exercise with a standardised post-exercise meal [FedEx + Meal], (iv) fed exercise without a standardised post-exercise meal [FedEx + NoMeal]. Studies must have measured ad libitum meal energy intake, within-lab energy intake, 24-h energy intake, energy expenditure, subjective hunger, acyl-ghrelin, peptide YY, and/or glucagon-like peptide 1. Random-effect network meta-analyses were performed for outcomes containing ≥5 studies.

Results
17 published articles (23 studies) were identified. Ad libitum meal energy intake was significantly lower during FedEx + Meal compared to FedEx + NoMeal (MD: −489 kJ; 95% CI, −898 to −80 kJ; P = 0.019). Within-lab energy intake was significantly lower during FastEx + NoMeal compared to FedEx + NoMeal (MD: −1326 kJ; 95% CI, −2102 to −550 kJ; P = 0.001). Similarly, 24-h energy intake following FastEx + NoMeal was significantly lower than FedEx + NoMeal (MD: −2095 kJ; 95% CI, −3910 kJ to −280 kJ; P = 0.024). Energy expenditure was however significantly lower during FastEx + NoMeal compared to FedEx+NoMeal (MD: −0.67 kJ/min; 95% CI, −1.10 to −0.23 kJ/min; P = 0.003). Subjective hunger was significantly higher during FastEx + Meal (MD: 13 mm; 95% CI, 5–21 mm; P = 0.001) and FastEx + NoMeal (MD: 23 mm; 95% CI, 16–30 mm; P < 0.001) compared to FedEx + NoMeal.

Conclusion
FastEx + NoMeal appears to be the most effective strategy to produce a short-term decrease in energy intake, but also results in increased hunger and lowered energy expenditure. Concerns regarding experimental design however lower the confidence in these findings, necessitating future research to rectify these issues when investigating exercise meal timing and energy balance.

PROSPERO registration number
CRD42020208041.

Key points

Fed exercise with a standardised post-exercise meal resulted in the lowest energy intake at the ad libitum meal served following exercise completion.

Fasted exercise without a standardised post-exercise meal resulted in the lowest within-lab and 24-h energy intake, but also produced the lowest energy expenditure and highest hunger.

Methodological issues lower the confidence in these findings and necessitate future work to address identified problems
A unique role of p53 haploinsufficiency or loss in the development of acute myeloid leukemia with FLT3-ITD mutation
Authors: Yang Min , Pan Zengkai , Huang Kezhi , Büsche Guntram , Liu Hongyun , Göhring Gudrun , Rumpel Regina , Dittrich-Breiholz Oliver , Talbot Steven , Scherr Michaela , Chaturvedi Anuhar , Eder Matthias , Skokowa Julia , Zhou Jianfeng , Welte Karl , von Neuhoff Nils , Liu Ligen , Ganser Arnold , Li Zhixiong 
Published: November 2021

AbstractWith an incidence of ~50%, the absence or reduced protein level of p53 is much more common than TP53 mutations in acute myeloid leukemia (AML). AML with FLT3-ITD (internal tandem duplication) mutations has an unfavorable prognosis and is highly associated with wt-p53 dysfunction. While TP53 mutation in the presence of FLT3-ITD does not induce AML in mice, it is not clear whether p53 haploinsufficiency or loss cooperates with FLT3-ITD in the induction of AML. Here, we generated FLT3-ITD knock-in; p53 knockout (heterozygous and homozygous) double-transgenic mice and found that both alterations strongly cooperated in the induction of cytogenetically normal AML without increasing the self-renewal potential. At the molecular level, we found the strong upregulation of Htra3 and the downregulation of Lin28a, leading to enhanced proliferation and the inhibition of apoptosis and differentiation. The co-occurrence of Htra3 overexpression and Lin28a knockdown, in the presence of FLT3-ITD, induced AML with similar morphology as leukemic cells from double-transgenic mice. These leukemic cells were highly sensitive to the proteasome inhibitor carfilzomib. Carfilzomib strongly enhanced the activity of targeting AXL (upstream of FLT3) against murine and human leukemic cells. Our results unravel a unique role of p53 haploinsufficiency or loss in the development of FLT3-ITD + AML.
Progress of shrink polymer micro- and nanomanufacturing
Authors: He Wenzheng , Ye Xiongying , Cui Tianhong 
Published: November 2021

AbstractTraditional lithography plays a significant role in the fabrication of micro- and nanostructures. Nevertheless, the fabrication process still suffers from the limitations of manufacturing devices with a high aspect ratio or three-dimensional structure. Recent findings have revealed that shrink polymers attain a certain potential in micro- and nanostructure manufacturing. This technique, denoted as heat-induced shrink lithography, exhibits inherent merits, including an improved fabrication resolution by shrinking, controllable shrinkage behavior, and surface wrinkles, and an efficient fabrication process. These merits unfold new avenues, compensating for the shortcomings of traditional technologies. Manufacturing using shrink polymers is investigated in regard to its mechanism and applications. This review classifies typical applications of shrink polymers in micro- and nanostructures into the size-contraction feature and surface wrinkles. Additionally, corresponding shrinkage mechanisms and models for shrinkage, and wrinkle parameter control are examined. Regarding the size-contraction feature, this paper summarizes the progress on high-aspect-ratio devices, microchannels, self-folding structures, optical antenna arrays, and nanowires. Regarding surface wrinkles, this paper evaluates the development of wearable sensors, electrochemical sensors, energy-conversion technology, cell-alignment structures, and antibacterial surfaces. Finally, the limitations and prospects of shrink lithography are analyzed.
miR-497-5p/SALL4 axis promotes stemness phenotype of choriocarcinoma and forms a feedback loop with DNMT-mediated epigenetic regulation
Authors: Peng Zheng , Zhang Yi , Shi Dazun , Jia Yanyan , Shi Huirong , Liu Huining 
Published: November 2021

AbstractChoriocarcinoma stem-like cells (CSLCs) might be at the origin of choriocarcinoma development associated with drug resistance or relapse. Spalt-like transcription factor 4 (SALL4), which is considered to be a stemness-related gene, can be regulated by miRNAs. In this study, SALL4 result is associated with progression-free survival of choriocarcinoma patients and CSLC’s stemness characteristics. In addition, it could be downregulated by miR-497-5p by direct binding. miR-497-5p silencing by hypermethylation promoted malignant CSLC phenotype in vitro and in vivo. Furthermore, increased DNA methyltransferases (DNMTs) by SALL4 upregulation inhibited miR-497-5p expression via hypermethylation promotion. SALL4 appeared to be a key factor in promoting stemness phenotype of choriocarcinoma. Silencing miR-497-5p and SALL4 promotes choriocarcinoma progression and forms a feedback loop with DNMT-mediated epigenetic regulation, playing a crucial role in stemness maintenance in choriocarcinoma.
Single-cell RNA transcriptome reveals the intra-tumoral heterogeneity and regulators underlying tumor progression in metastatic pancreatic ductal adenocarcinoma
Authors: Xu Qianhui , Chen Shaohuai , Hu Yuanbo , Huang Wen 
Published: November 2021

AbstractPancreatic ductal adenocarcinoma (PDAC) is the most frequent and aggressive pancreatic tumor characterized by high metastatic risk and special tumor microenvironment. To comprehensively delineate the complex intra-tumoral heterogeneity and the underlying mechanism during metastatic lesions malignant progression, single-cell RNA sequencing (scRNA-seq) was employed. PCA and TSNE were used for dimensionality reduction analysis and cell clustering. Find All Markers function was used to calculate differential genes in each cluster, and Do Heatmap function was used to plot the distribution of differential genes in each cluster. GSVA was employed to assign pathway activity estimates to individual cells. Lineage trajectory progression was inferred by monocle. CNV status was inferred to compare the heterogeneity among patients and subtypes by infercnv. Ligand-receptor interactions were identified by CellPhoneDB, and regulons network of cells was analyzed by SCENIC. Through RNA-sequencing of 6236 individual cells from 5 liver metastatic PDAC lesions, 10 major cell clusters are identified by using unbiased clustering analysis of expression profiling and well-known cell markers. Cells with high CNV level were considered as malignant cells and pathway analyses were carried out to highlight intratumor heterogeneity in PDAC. Pseudotime trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. The complex cellular communication suggested potential immunotherapeutic targets in PDAC. Regulon network identified multiple candidates for promising cell-specific transcriptional factors. Finally, metastatic-related genes expression levels and signaling pathways were validated in bulk RNA Sequencing data. This study contributed a comprehensive single-cell transcriptome atlas and contributed into novel insight of intratumor heterogeneity and molecular mechanism in metastatic PDAC.
Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression
Authors: Zhu Pan , Lister Clare , Dean Caroline 
Published: November 2021

AbstractPlants use seasonal temperature cues to time the transition to reproduction. In Arabidopsis thaliana, winter cold epigenetically silences the floral repressor locus FLOWERING LOCUS C (FLC) through POLYCOMB REPRESSIVE COMPLEX 2 (PRC2)1. This vernalization process aligns flowering with spring. A prerequisite for silencing is transcriptional downregulation of FLC, but how this occurs in the fluctuating temperature regimes of autumn is unknown2–4. Transcriptional repression correlates with decreased local levels of histone H3 trimethylation at K36 (H3K36me3) and H3 trimethylation at K4 (H3K4me3)5,6, which are deposited during FRIGIDA (FRI)-dependent activation of FLC7–10. Here we show that cold rapidly promotes the formation of FRI nuclear condensates that do not colocalize with an active FLC locus. This correlates with reduced FRI occupancy at the FLC promoter and FLC repression. Warm temperature spikes reverse this process, buffering FLC shutdown to prevent premature flowering. The accumulation of condensates in the cold is affected by specific co-transcriptional regulators and cold induction of a specific isoform of the antisense RNA COOLAIR5,11. Our work describes the dynamic partitioning of a transcriptional activator conferring plasticity in response to natural temperature fluctuations, thus enabling plants to effectively monitor seasonal progression.
B cell-derived GABA elicits IL-10+ macrophages to limit anti-tumour immunity
Authors: Zhang Baihao , Vogelzang Alexis , Miyajima Michio , Sugiura Yuki , Wu Yibo , Chamoto Kenji , Nakano Rei , Hatae Ryusuke , Menzies Rosemary J. , Sonomura Kazuhiro , Hojo Nozomi , Ogawa Taisaku , Kobayashi Wakana , Tsutsui Yumi , Yamamoto Sachiko , Maruya Mikako , Narushima Seiko , Suzuki Keiichiro , Sugiya Hiroshi , Murakami Kosaku , Hashimoto Motomu , Ueno Hideki , Kobayashi Takashi , Ito Katsuhiro , Hirano Tomoko , Shiroguchi Katsuyuki , Matsuda Fumihiko , Suematsu Makoto , Honjo Tasuku , Fagarasan Sidonia 
Published: November 2021

AbstractSmall, soluble metabolites not only are essential intermediates in intracellular biochemical processes, but can also influence neighbouring cells when released into the extracellular milieu1–3. Here we identify the metabolite and neurotransmitter GABA as a candidate signalling molecule synthesized and secreted by activated B cells and plasma cells. We show that B cell-derived GABA promotes monocyte differentiation into anti-inflammatory macrophages that secrete interleukin-10 and inhibit CD8+ T cell killer function. In mice, B cell deficiency or B cell-specific inactivation of the GABA-generating enzyme GAD67 enhances anti-tumour responses. Our study reveals that, in addition to cytokines and membrane proteins, small metabolites derived from B-lineage cells have immunoregulatory functions, which may be pharmaceutical targets allowing fine-tuning of immune responses.
Neural networks can learn to utilize correlated auxiliary noise
Authors: Ahmadzadegan Aida , Simidzija Petar , Li Ming , Kempf Achim 
Published: November 2021

AbstractWe demonstrate that neural networks that process noisy data can learn to exploit, when available, access to auxiliary noise that is correlated with the noise on the data. In effect, the network learns to use the correlated auxiliary noise as an approximate key to decipher its noisy input data. An example of naturally occurring correlated auxiliary noise is the noise due to decoherence. Our results could, therefore, also be of interest, for example, for machine-learned quantum error correction.
Changes in microbial community and enzyme activity in soil under continuous pepper cropping in response to Trichoderma hamatum MHT1134 application
Authors: Mao Tingting , Jiang Xuanli 
Published: November 2021

AbstractTo clarify the control effects of Trichoderma hamatum strain MHT1134 on Fusarium wilt in continuous pepper cropping fields and its regulatory effects on soil microecology, the physical and chemical properties, enzyme activities, community structures of soil samples from five field types were analysed. Samples were taken from fields that had been continuously planted for 1, 5, 9 years, and applied the strain MHT1134 for 1 and 2 years. The MHT1134 control effects on pepper wilt after application 1 year and 2 years were 63.03% and 70.21%, respectively. 4 kinds of physical and chemical indexes and 6 kinds of enzyme activities in soil were increased. With the continuous cropping years increased, the microbial abundance and diversity decreasing significantly. The relative abundances of Fusarium, Gibberella increased along with the planting years, but decreased after the MHT11134 application. However, the relative abundances of Trichoderma and Chaetomium significantly increased. Additionally, as the cropping years increased, the soil abundance of Actinobacteria gradually decreased, but it significantly increased from 17.56 to 22.44% after the MHT1134 application. Thus, strain MHT1134 effectively improved the microbial community structure of the soil, and it also positively affected soil quality. A continuous application may improve the control effect.