bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactive edible film based on Konjac glucomannan and probiotic Lactobacillus plantarum strains: Physicochemical properties and shelf life of fresh‐cut kiwis
Authors: Hashemi Seyed Mohammad Bagher , Jafarpour Dornoush 
Published: January 2021

AbstractThis study investigates the efficacy of Lactobacillus plantarum strains (L. plantarum LP3, L. plantarum AF1, and L. plantarum LU5) incorporated into a Konjac‐based edible coating in order to prevent fungi growth and retain physicochemical characteristics of fresh‐cut kiwis kept at 4 °C for 5 days. For this purpose, probiotic survivability, fungi counts, decay percentage, color changes, titratable acidity (TA), total soluble solids (TSS), ascorbic acid content, chlorophyll amount, total phenolics, and DPPH radical scavenging of fresh‐cut kiwis were evaluated. Results indicated that the population of L. plantarum strains in all treated groups retained at sufficient amounts of probiotic consumption (above 6 and 7 log CFU/g) at the end of the storage period and L. plantarum LP3 had the highest viability in comparison to other strains. The incorporation of L. plantarum in Konjac coatings markedly reduced the amount of decay and color changes and maintained the chlorophyll and ascorbic acid contents of fresh‐cut kiwis compared to control samples. After 5 days of storage, total phenol content and the DPPH antiradical activities of coated kiwi slices treated with probiotics were observed about 1.2 and 10.23 g/kg compared to the pure Konjac‐coated (0.84 and 7.6 g/kg) and Konjac‐uncoated samples (0.44 and 4.1 g/kg), respectively. No significant difference in TSS and TA of various treatments was detected. Coated kiwi slices loaded with probiotics had higher overall acceptability compared to Konjac‐coated and control samples. In addition, probiotic treatment significantly reduced mold and yeast counts compared to the control group.Practical ApplicationsRecently, edible films have received more consideration as a promising method to enhance the shelf life of fresh‐cut fruit. The presence of probiotics in edible films reduces the growth of spoilage microorganisms and improves consumer health. Our findings encourage the application of edible coating incorporated with L. plantarum to design multifunctional foods and preserve the qualities of fresh‐cut kiwifruit.
The Development Characteristics and Distribution Predictions of the Paleogene Coal‐measure Source Rock in the Qiongdongnan Basin, Northern South China Sea
Authors: WANG Dongdong , ZHANG Gongcheng , LI Zengxue , DONG Guoqi , SUN Rui , GUO Shuai , WU Yue 
Published: January 2021

AbstractThere are known to be enormous Cenozoic coal‐type oil and gas resources located in the basins of the South China Sea, among which the Paleogene coal‐measure source rock are one of the main source rock. In order to more effectively analyze the distribution laws of coal‐measure source rock in marginal sea basins and guide coal‐type oil and gas explorations, the Oligocene coal‐measure source rock in the Qiongdongnan Basin were selected as examples in order to systematically analyze the types, development characteristics, control factors, and distribution prediction methods of coal‐measure source rock in marginal sea basins. The Qiongdongnan Basin is located in the northern region of the South China Sea. Previous explorations of the area have determined that the Oligocene coal‐measure source rock in the Qiongdongnan Basin have typical “binary structures”, which include coal seams and terrigenous marine mudstone. Among those, the terrigenous marine mudstone has been found to greatly expand the scope of the coal‐measure source rock. In addition, the coal seams which have been exposed by drilling have been observed to have the characteristics of thin single layer thickness, many layers, and poor stability. Meanwhile, the terrigenous marine mudstone has the characteristics of large thickness and wide distribution. The development of coal‐measure source rock is known to be controlled by many factors, such as paleoclimate and paleobotany condition, paleo‐structure and topography, paleo‐geography, rise and fall of base level, and so on. In accordance with the comprehensive analyses of various control factors of coal‐measure source rock, and the changes in water body energy in sedimentary environments, a genetic model of the “energy belt” for the development and distribution of coal‐measure source rock was proposed. Also, the development and distribution characteristics of coal‐measure source rock in different types of energy belts were clarified. Then, based on the development and distribution characteristics of coal‐measure source rock and their controlling factors, prediction methods of the distribution ranges of coal‐measure source rock were proposed from both qualitative and (semi‐) quantitative aspects. That is to say, a method for (semi‐) quantitative predictions of the distribution ranges of coal‐bearing intervals based on model wave impedance inversion and neural network wave impedance inversion, along with a method for (semi‐) quantitative predictions of the distribution ranges of terrigenous marine mudstone based on topographical slopes, were introduced in this study.
The role of genital mycoplasma infection in female infertility: A systematic review and meta‐analysis
Authors: Tantengco Ourlad Alzeus G. , de Castro Silva Mariana , Velayo Clarissa L. 
Published: January 2021

AbstractProblemRecent studies show that lower genital tract infection with genital mycoplasma may be associated with the pathology of female infertility. However, this association remains controversial due to the variable prevalence, sample sizes, and different methods used to diagnose genital mycoplasma infection. The aim of the present meta‐analysis was to gain better understanding of the specific impact of genital mycoplasma on female infertility.Method of StudyA systematic review of literature on the association of genital mycoplasma (Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum) infection and female infertility was performed using three electronic databases: PubMed, Scopus, and CINAHL, from January 2000 to January 2020. Pooled odds ratio (OR) and 95% confidence intervals for genital mycoplasma infection and female infertility were derived from a fixed effects model.ResultsThis meta‐analysis included eight studies conducted in six countries. Based on the results, women with infertility had a statistically higher odds of having any genital mycoplasma infection (p < .0001) compared to the control group. The pooled OR of all the included studies was 3.82 (95% CI: 2.55, 5.72). There was an unremarkable heterogeneity in all the studies included in this meta‐analysis (I2 = 0%, p = .48). A subgroup analysis also showed that M. genitalium, M. hominis, and U. urealyticum infections are significantly associated with female infertility.ConclusionOur meta‐analysis showed a significant association between M. genitalium, M. hominis, and U. urealyticum infections and female infertility. This evidence supports the development of guidelines for the diagnosis and treatment of genital mycoplasma infections to prevent female infertility.
Mechanisms of oral immunotherapy
Authors: Barshow Suzanne M. , Kulis Michael D. , Burks A. Wesley , Kim Edwin H. 
Published: January 2021

AbstractFood allergy presents a significant global health concern with up to 10% of the population affected in developed nations and a steadily increasing prevalence. In many cases, particularly with peanut, tree nut and shellfish, food allergy is a lifelong and potentially life‐threatening diagnosis. While no ‘cure’ for IgE‐mediated food allergy exists, oral immunotherapy (OIT) is a promising treatment modality with the peanut OIT drug Palforzia (Aimmune Therapeutics) the only treatment for food allergy that is currently approved by the United States Food and Drug Administration. OIT primarily induces a state of desensitization with only a minority of subjects achieving sustained unresponsiveness, a state of limited clinical remission that appears to be immunologically distinct from natural tolerance. Early humoural changes during OIT include an initial increase in allergen‐specific IgE, which eventually decreases to below baseline levels as OIT progresses, and a gradual increase in allergen‐specific IgA and IgG4 that continues throughout the course of OIT. Basophil hyporesponsiveness and decreased skin prick test wheal size are observed within the first year of OIT, and persistence after completion of therapy has been associated with sustained unresponsiveness. In the T‐cell compartment, there is an initial expansion followed by a decline in the number and activity of T helper 2 (TH2) cells, the latter of which may be dependent on an expansion of IL‐10‐producing cells, including regulatory T‐cells. Our understanding of the immunomodulatory effects of OIT continues to evolve, with new technologies such as single‐cell transcriptional profiling and antibody epitope analysis allowing for more detailed study of T‐cell and B‐cell responses to OIT. In this review, we present evidence to illustrate what is currently known about the immunologic changes induced by OIT, explore potential mechanisms and emphasize knowledge gaps where future research is needed.
Systematic screening for late sequelae after colorectal cancer—a feasibility study
Authors: Juul Therese , Bräuner Annette Boesen , Drewes Asbjørn Mohr , Emmertsen Katrine Jøssing , Krogh Klaus , Laurberg Søren , Lauritzen Michael Bødker , Thorlacius‐Ussing Ole , Christensen Peter ,   Danish Cancer Society Centre for Research on Survivorship and Late Adverse Effects after Cancer in the Pelvic Organs Study Group
Published: January 2021

AbstractAimThe aim of this study was to test the feasibility of a new method for systematic screening for late sequelae (LS) following colorectal cancer treatment.MethodPatients with colorectal cancer from five Danish hospitals were invited to complete a survey about LS at 3, 12, 24 and 36 months after surgery as part of their follow‐up. The survey consisted primarily of validated tools, supplemented by a few ad hoc items, measuring bowel, urinary and sexual dysfunction, pain and quality of life and an additional question regarding request for contact. Patients completed surveys electronically or on paper.ResultsOf the 1721 invited patients, 1386 (80.5%) were included (1085 with colon cancer and 301 with rectal cancer) of whom 72.5% responded electronically. Patients responding electronically were 7.6 years younger than those responding on paper (P < 0.001). Since some patients answered more than once, the dataset consisted of 2361 surveys. Patients with colon cancer requested phone contact in 19.0% of the surveys, and 8.4% were referred to treatment for LS, primarily bowel dysfunction. Among patients with rectal cancer, 30.8% requested phone contact and 16.2% were referred for treatment of LS, mainly due to bowel and sexual dysfunction.ConclusionThis is the first paper investigating a new method of systematic screening for LS following colorectal cancer using electronic patient‐reported outcome measures. The study shows that in the Danish population a high response rate can be obtained with this method and that close to three‐quarters of patients respond electronically. Patients with rectal cancer had a higher need for phone contact and treatment of LS than patients with colon cancer.
Hindcasting multidecadal predevelopment groundwater levels in the Floridan aquifer
Authors: Gordu Fatih , Nachabe Mahmood H. 
Published: January 2021

AbstractEstablishing predevelopment benchmark groundwater conditions is challenging without long‐term records to discern impacts of pumping and climate change on aquifer levels. Understanding periodic natural cycles and trends require 100 years or more data which rarely exist. Using limited records, we develop an approach to hindcast multidecadal levels and examine the temporal evolution of climatic and pumping impacts. The methodology includes a wavelet‐aided statistical model, constrained by temporal scales of physical processes responsible for groundwater level variation, including rainfall, evapotranspiration and pumping stresses. The model and hindcasts are tested at three sites in Florida using traditional split calibration‐verification methods for the period of record and with the documented historical drought and wet years for the period of no‐record. The pumping impact is quantified over time and compared with regional groundwater models, revealing that withdrawals are responsible for 30 to 70% of the declines in levels since 1960s. Hindcasting yielding 110 years of monthly levels is used to assess the effect of climate change and pumping on the frequency of critical low levels. At all three sites, the frequencies of critical low levels increase significantly in the 1960 to 2015 period when compared to the 1904 to 1959 period. For example, at site 1, the return period of the critical low level is shortened by 3.9 years due to climate change and 2.2 years due to pumping.
Secondary stabbing headache associated with intracranial tumors, aneurysms, and arteriovenous malformation: An alarming warning sign
Authors: Valença Marcelo Moraes , de Azevedo Filho Hildo Rocha Cirne , de Souza Ferreira Maria Rosana , Valença Marcelo Andrade , Krymchantowski Abouch Valenty , Valença Martina Falcão , Andrade‐Valença Luciana Patrízia Alves 
Published: January 2021

AbstractBackgroundStabbing headache (SH) is considered as a pure primary headache, but according to a few clinical observations it could also be secondary. Over the past decades, we have been observing the complaint of SH in patients with intracranial vascular and neoplastic lesions.ObjectiveTo describe a series of patients with intracranial lesions who experienced SH.MethodsThis is a cross‐sectional, retrospective study of 34 patients with intracranial lesions associated with SH, admitted at Hospital das Clínicas, Federal University of Pernambuco, Brazil.ResultsIn this series of 34 patients [29 women, 44 ± 12 years (mean ± SD)] with secondary SH, the causes were intracranial neoplasms (n = 31), cerebral aneurysms (n = 2), or arteriovenous malformation (n = 1). Pituitary tumor (n = 18), meningioma (n = 6), and vestibular schwannomas (n = 4) were the most prevalent types of intracranial neoplasms. All these lesions had intimate contact with the dura mater, including an oligodendroglioma, the only intra‐axial tumor in the series. A characteristic in the secondary SH is the crescendo pattern (12/34, 35%), progressing from infrequent attacks to recurrent crises occurring several times a day. The SH lasted from 5 days to 60 months (15 ± 18 months, mean ± SD) until the correct diagnosis [16/34 (47%) of the patients ≤6 months]. The SH was triggered by the movement of the head (5/34, 15%) or Valsalva maneuver (1/34). After surgery, suppression of the SH was observed. In a few of the patients to whom dexamethasone was prescribed, the SH subsided within a few days.ConclusionThis study was able to identify clinical red flags associated with intracranial lesions and secondary SH, for example, recent onset of SH, exclusively unilateral (ipsilateral) at the same location, crescendo pattern, triggered by head movements, or Valsalva maneuver.
Association of serum CTRP9 levels with cardiac autonomic neuropathy in patients with type 2 diabetes mellitus
Authors: Yang Junpeng , Zhao Dongni , Chen Yi , Ma Yuehua , Shi Xiaoyang , Wang Xiaobing , Lv Yinghua , Yuan Huijuan 
Published: January 2021

AbstractAimsCardiac autonomic neuropathy (CAN) is a serious complication of diabetes and is associated with adipokines. The C1q tumor necrosis factor‐related protein 9 (CTRP9) is a newly discovered adipokine. This study aimed to evaluate the association of serum CTRP9 levels with the prevalence and severity of CAN in patients with type 2 diabetes mellitus.Materials and MethodsWe enrolled 262 patients (aged ≥18 years) with type 2 diabetes mellitus into this study. Standard cardiovascular autonomic reflex tests (CARTs) were used to assess CAN and patients were divided into three groups accordingly: a non‐CAN group, an early CAN group, and a definite CAN group. Serum CTRP9 levels were measured by enzyme‐linked immunosorbent assay, and the tertiles were calculated.ResultsSerum CTRP9 levels decreased significantly in the early CAN and definite CAN groups (P < 0.05). The percentage of definite CAN was the highest at the minimum tertile of serum CTRP9 level (T1; P < 0.05). Additionally, serum CTRP9 levels were negatively correlated with age, DM duration, hemoglobin A1c (HbA1c), and fasting plasma glucose (FPG) while positively correlated with high‐density lipoprotein cholesterol (HDL; P < 0.05). The level of CTRP9 was also significantly associated with the four indexes of CARTs (P < 0.05). Furthermore, CTRP9 was a protective factor for definite CAN (P < 0.05). Compared with the maximum tertile (T3) of the serum CTRP9 levels, a decreased level of serum CTRP9 in T1 significantly increased the prevalence ratio of definite CAN in patients with type 2 diabetes mellitus (P < 0.05).ConclusionSerum CTRP9 levels were independently associated with definite CAN. CTRP9 represents a reliable biomarker for exploring CAN in patients with type 2 diabetes mellitus.
Glutamate is an essential mediator in glutamine‐amplified insulin secretion
Authors: Han Guirong , Takahashi Harumi , Murao Naoya , Gheni Ghupurjan , Yokoi Norihide , Hamamoto Yoshiyuki , Asahara Shun‐ichiro , Seino Yutaka , Kido Yoshiaki , Seino Susumu 
Published: January 2021

AbstractAims/IntroductionGlutamine is the most abundant amino acid in the circulation. In this study, we investigated cell signaling in the amplification of insulin secretion by glutamine.Materials and MethodsClonal pancreatic β‐cells MIN6‐K8, wild‐type B6 mouse islets, glutamate dehydrogenase (GDH) knockout clonal β‐cells (Glud1KOβCL), and glutamate‐oxaloacetate transaminase 1 (GOT1) knockout clonal β‐cells (Got1KOβCL) were studied. Insulin secretion from these cells and islets was examined under various conditions, and intracellular glutamine metabolism was assessed by metabolic flux analysis. Intracellular Ca2+ concentration ([Ca2+]i) was also measured.ResultsGlutamine dose‐dependently amplified insulin secretion in the presence of high glucose in both MIN6‐K8 cells and Glud1KOβCL. Inhibition of glutaminases, the enzymes that convert glutamine to glutamate, dramatically reduced the glutamine‐amplifying effect on insulin secretion. A substantial amount of glutamate was produced from glutamine through direct conversion by glutaminases. Glutamine also increased [Ca2+]i at high glucose, which was abolished by inhibition of glutaminases. Glutamic acid dimethylester (dm‐Glu), a membrane permeable glutamate precursor that is converted to glutamate in cells, increased [Ca2+]i as well as induced insulin secretion at high glucose. These effects of glutamine and dm‐Glu were dependent on calcium influx. Glutamine also induced insulin secretion in clonal β‐cells MIN6‐m14, which otherwise exhibit no insulin secretory response to glucose.ConclusionsGlutamate converted from glutamine is an essential mediator that enhances calcium signaling in the glutamine‐amplifying effect on insulin secretion. Our data also suggest that glutamine exerts a permissive effect on glucose‐induced insulin secretion.
Eo‐Variscan metamorphism in the Bohemian Massif: Thermodynamic modelling and monazite geochronology of gneisses and granulites of the Góry Sowie Massif, SW Poland
Authors: Jastrzębski Mirosław , Budzyń Bartosz , Żelaźniewicz Andrzej , Konečný Patrik , Sláma Jiří , Kozub‐Budzyń Gabriela A. , Skrzypek Etienne , Jaźwa Aleksandra 
Published: January 2021

AbstractIn the Variscides of central Europe, the tectonothermal history of a few units jointly assigned to the Teplá‐Barrandian/Bohemian microplate had already terminated in the Devonian. One of these is the Góry Sowie Massif (GSM; Sudetes, SW Poland), mainly consisting of variably migmatitic gneisses with subordinate granulite and peridotite inserts. The eo‐Variscan evolution of the GSM was completed in five tectonothermal stages; however, the timing and pressure–temperature (P–T) conditions of the individual stages remain unclear because isotopic ages overlap within error, and previousP–Testimates have been determined without reference to these ages. Our thermodynamic modelling and LA–ICP–MS U–Pb and electron probe microanalysis (EPMA) U–Th–total Pb monazite geochronology provide new insights into the early Variscan multistage evolution of the GSM. The newP–T–time (t) data show that granulites were metamorphosed concurrently with gneisses but at different depths and temperatures. During subduction of Saxothuringia beneath Teplá‐Barrandia at 395–390 Ma, the granulites of crustal provenance experienced high‐P–high‐Tconditions (740–750°C, 17–18 kbar) and came in contact with the overlying mantle. Slab break‐off gave rise to decompression from conditions of ~17 kbar/840°C to 11–12 kbar/850–860°C, which continued at 380–370 Ma when the granulites were sheared off and extruded as tectonic slices to lower–middle crustal depths, where gneisses were undergoing metamorphism and migmatization under amphibolite facies conditions. In the gneisses, the oldest monazite date ofc. 390 Ma constrains the age of the early tectonometamorphic event D1. Two subsequent events (D2and D3, sillimanite grade) occurred under conditions of 660–680°C and 5–9 kbar at 380–375 Ma, when, in various parts of the future massif, the gneisses were (re)folded and migmatized due to heat arising from underplated mantle. Isostatic exhumation was accompanied by pegmatite intrusions (D4) at 370 Ma and subsequent orogenic collapse (D5). Concurrently, high temperature conditions (~700°C) promoted a disturbance of the U–Pb system in monazite in some granulites.
BED domain‐containing NLR from wild barley confers resistance to leaf rust
Authors: Chen Chunhong , Jost Matthias , Clark Bethany , Martin Matthew , Matny Oadi , Steffenson Brian J. , Franckowiak Jerome D. , Mascher Martin , Singh Davinder , Perovic Dragan , Richardson Terese , Periyannan Sambasivam , Lagudah Evans S. , Park Robert F. , Dracatos Peter M. 
Published: January 2021

SummaryLeaf rust, caused by Puccinia hordei, is a devastating fungal disease affecting barley (Hordeum vulgare subsp. vulgare) production globally. Despite the effectiveness of genetic resistance, the deployment of single genes often compromises durability due to the emergence of virulent P. hordei races, prompting the search for new sources of resistance. Here we report on the cloning of Rph15, a resistance gene derived from barley’s wild progenitor H. vulgare subsp. spontaneum. We demonstrate using introgression mapping, mutation and complementation that the Rph15 gene from the near‐isogenic line (NIL) Bowman + Rph15 (referred to as BW719) encodes a coiled‐coil nucleotide‐binding leucine‐rich repeat (NLR) protein with an integrated Zinc finger BED (ZF‐BED) domain. A predicted KASP marker was developed and validated across a collection of Australian cultivars and a series of introgression lines in the Bowman background known to carry the Rph15 resistance. Rph16 from HS‐680, another wild barley derived leaf rust resistance gene, was previously mapped to the same genomic region on chromosome 2H and was assumed to be allelic with Rph15 based on genetic studies. Both sequence analysis, race specificity and the identification of a knockout mutant in the HS‐680 background suggest that Rph15‐ and Rph16‐mediated resistances are in fact the same and not allelic as previously thought. The cloning of Rph15 now permits efficient gene deployment and the production of resistance gene cassettes for sustained leaf rust control.