bluuebunny/crossref_metadata_2025_split huggingface dataset:
Locoregional Recurrence via Mucus-Mediated Extension Following Lung Resection for Mucinous Tumors
Authors: Kawaguchi Yo , Hanaoka Jun , Ohshio Yasuhiko , Okamoto Keigo , Kaku Ryosuke , Hayashi Kazuki , Shiratori Takuya , Akazawa Akira , Ishida Mitsuaki 
Published: March 2021

Abstract
Background

Clinically, locoregional recurrences following mucinous tumor resection are often experienced. However, it is unclear whether mucinous tumors directly affect local recurrence or not, and if so, what the mechanism is. Therefore, we investigated whether mucinous tumors are associated with locoregional recurrence after pulmonary resection and whether mucus extension is a risk factor for locoregional recurrence.
Methods

The data of 90 patients who underwent pulmonary resection for metastases were reviewed. If mucus was partially or wholly present in the tumor based on macro- or microscopic identification, we assigned the tumor as mucinous. In mucinous tumors, if mucus was identified within the air spaces in the normal lung parenchyma, beyond the edge of the tumor, we assigned the tumor as positive for “mucus extension.”
Results

The 5-year cumulative incidence of locoregional recurrence in patients with mucinous tumors was 80.3%, which was significantly higher than the 15.5% observed in patients with non-mucinous tumors. Within the mucinous tumor, presence of mucus extension beyond the tumor edge was an independent risk factor for locoregional recurrence after pulmonary resection (hazard ratio, 8.08; P = 0.049).
Conclusion

During the resection of mucinous cancer, surgeons should maintain sufficient distance from the tumor edge to prevent locoregional recurrences.
Decreased Expression of Brg1 and β-Catenin in Nasal Epithelial Cells in CRSwNPs
Authors: Tao Yanli , Zhu Huitao , Liu Chengcheng , Sun Xiaoyan , Yuan Tiejun , Zhang Hongping , Zhang Hailing , Zhao Li , Jin Peng , Yu Kena , Shi Li , Xia Ming 
Published: March 2021

Abstract
Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a pathophysiologically complex disease process characterized by chronic inflammation of the nose and epithelial cell structure remodeling. Objectives: Studies have shown that the chromatin remodeling complex subunit Brg1 can interact with β-catenin, which plays an important role in cell proliferation and differentiation. However, the role of Brg1 and β-catenin in epithelial cells of chronic rhinitis and sinusitis remains unclear. Material and Methods: We detected the expression level of Brg1 in CRSwNP by qPCR, and observed the expression pattern of Brg1 in CRSwNP by immunohistochemistry and immunofluorescence. HNEpC cells were used to detect the expression changes of the two proteins after TNF-α stimulation. Results: In our study, the expression levels of Brg1 and β-catenin are significantly decreased in nasal mucosa of CRSwNP patients. To our interest, we also observed expression decrease and expression pattern changes of Brg1 and β-catenin by stimulating HNEpCs cells with TNF-α. Conclusions: This study shows that the expression of Brg1 and β-catenin may be related to the development of CRSwNPs.Subject classification codes: include these here if the journal requires them
Silica-Coated Magnetic Nanoparticles Activate Microglia and Induce Neurotoxic D-Serine Secretion
Authors: Shin Tae Hwan , Lee Da Yeon , Manavalan Balachandran , Basith Shaherin , Na Yun-Cheol , Yoon Cheolho , Lee Hyeon-Seong , Paik Man Jeong , Lee Gwang 
Published: March 2021

Abstract
Background
Nanoparticles have been studied for brain imaging, diagnosis, and drug delivery owing to their versatile properties due to their small sizes. However, there are growing concerns that nanoparticles may exert toxic effects in the brain. In this study, we assessed direct nanotoxicity on microglia, the resident macrophages of the central nervous system, and indirect toxicity on neuronal cells exerted by silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO2(RITC)].
Methods
We investigated MNPs@SiO2(RITC)-induced biological changes in BV2 murine microglial cells via RNA-sequencing-based transcriptome analysis and gas chromatography-mass spectrometry-based intracellular and extracellular amino acid profiling. Morphological changes were analyzed by transmission electron microscopy. Indirect effects of MNPs@SiO2(RITC) on neuronal cells were assessed by Transwell-based coculture with MNPs@SiO2(RITC)-treated microglia. MNPs@SiO2(RITC)-induced biological changes in the mouse brain in vivo were examined by immunohistochemical analysis.
Results
BV2 murine microglial cells were morphologically activated and the expression of Iba1, an activation marker protein, was increased after MNPs@SiO2(RITC) treatment. transmission electron microscopy analysis revealed lysosomal accumulation of MNPs@SiO2(RITC) and the formation of vesicle-like structures in MNPs@SiO2(RITC)-treated BV2 cells. The expression of several genes related to metabolism and inflammation were altered in 0.1 µg/µl MNPs@SiO2(RITC)-treated microglia when compared with that in non-treated (control) and 0.01 µg/µl MNPs@SiO2(RITC)-treated microglia. Combined transcriptome and amino acid profiling analyses revealed that the transport of serine family amino acids, including glycine, cysteine, and serine, was enhanced. However, only serine was increased in the growth medium of activated microglia; especially, excitotoxic d-serine secretion from primary rat microglia was the most strongly enhanced. Activated primary microglia reduced intracellular ATP levels and proteasome activity in cocultured neuronal cells, especially in primary cortical neurons, via d-serine secretion. Moreover, ubiquitinated proteins accumulated and inclusion bodies were increased in primary dopaminergic and cortical neurons cocultured with activated primary microglia. In vivo, MNPs@SiO2(RITC), d-serine, and ubiquitin aggresomes were distributed in the MNPs@SiO2(RITC)-treated mouse brain.
Conclusions
MNPs@SiO2(RITC)-induced activation of microglia triggers excitotoxicity in neurons via d-serine secretion, highlighting the importance of neurotoxicity mechanisms incurred by nanoparticle-induced microglial activation.
Does Green Credit Affect the Green Innovation Performance of High-Polluting and Energy-Intensive Enterprises? Evidence from A Quasi-Natural Experiment
Authors: Liu Sheng , XU RONGXIN , Chen Xiuying 
Published: March 2021

Abstract
Green credit policy is a
practical exploration to guide green development through the
allocation of financial resources, but there is a gap in the
theoretical research on how green financial policy affects
enterprise green technology innovation. Taking the green credit
policy in 2012 as a quasi-natural experiment, this paper applies
the methods of Propensity Score Matching and Difference in
Difference (PSM-DID) to investigate the relationship between green
credit policy and enterprises' green technology innovation behavior
based on Chinese industrial enterprises database and green patent
database. The results show that the implementation of "green credit
guidelines" policy has significantly improved the green innovation
output of high-polluting and high-energy consuming enterprises,
which indicates that the incentive effect of green credit policy on
enterprises exceeds the inhibition effect and leads to Porter
effect. Moreover, the green credit policy has significantly
increased the number of non-invention patents rather than invention
patents. In addition, the green credit policy has a more
significant effect on the innovation output of heavily polluting
enterprises in state-owned and weak market power enterprises.
Mechanism test shows that green credit policy mainly affects the
green innovation output of heavy polluting enterprises by guiding
the loan financing cost and R&D investment allocation.
Gonadal Antioxidant Responses to Seawater Acidification and Hypoxia in the Marine Mussel Mytilus Coruscus
Authors: Xu Guangen , Kong Hui , Chang Xueqing , Chen Hui , Deng Yuewen , Hu Menghong , Wang Youji 
Published: March 2021

Abstract
This study investigated the combined effects of seawater acidification and hypoxia on the gonadal antioxidant response of the thick shell mussel Mytilus coruscus mainly distributed along the Shengsi Island, East China Sea, where hypoxia and pH fluctuations frequently occur in summer. Mussels were exposed to three pH levels (8.1, 7.7 and 7.3) and two dissolved oxygen (DO) levels (6 and 2 mg L− 1) for 21 days following a 10-day recovery. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione (GSH), glutathione S-transferase (GST) and malondialdehyde (MDA) in gonad and gonad surface area (GSA) were measured at day 21 and 31. Among all the parameters, there was no significant change in SOD activity. GSA and the activity of CAT and GST were decreased under acidification and hypoxia, but GPX, GSH and MDA were increased. PCA showed that the changes were influenced by pH more than DO. Interaction between acidification and hypoxia was found significant on GPX activity and GSA. Integrated biomarker response (IBR) analysis demonstrated that acidification and hypoxia impaired mussel’s antioxidant system and increased oxidative damage. Our results clearly showed that acidification and hypoxia synergistically exert negative impact on the antioxidant system and gonad development of mussels, and the effect of acidification was more significant.
Efficient and Accurate Electronic Structure Simulation Demonstrated on a Trapped-Ion Quantum Computer
Authors: Kawashima Yukio , Coons Marc , Nam Yunseong , Lloyd Erika , Matsuura Shunji , Garza Alejandro , Johri Sonika , Huntington Lee , Senicourt Valentin , Maksymov Andrii , Nguyen Jason , Kim Jungsang , Alidoust Nima , Zaribafiyan Arman , Yamazaki Takeshi 
Published: March 2021

Abstract
Quantum computers have the potential to perform accurate and efficient electronic structure calculations, enabling the simulation of properties of materials. However, today’s noisy, intermediate-scale quantum (NISQ) devices have a limited number of qubits and gate operations due to the presence of errors. Here, we propose a systematically improvable end-to-end pipeline to alleviate these limitations. Our proposed resource-efficient pipeline combines problem decomposition techniques for compact molecular representations, circuit optimization methods for compilation, solving the eigenvalue problem on advanced quantum hardware, and error-mitigation techniques in post-processing the results. Using the density matrix embedding theory for compact representation, and an ion-trap quantum computer, we simulate a ring of 10 hydrogen atoms taking into account all electrons equally and explicitly in the electronic structure calculation. In our experiment, we simulated the largest molecular system on a quantum computer within chemical accuracy with respect to total molecular energy calculated by the full CI method. Our methods reduce the number of qubits required for high-accuracy quantum simulations by an order of magnitude in the present work, enabling the simulation of larger, more industrially relevant molecules using NISQ devices. They are further systematically improvable as devices’ computational capacity continues to grow.
DDX3X Loss is Associated with Aggressive Phenotypes in Non-Hodgkin’s Lymphomas
Authors: Kizhakeyil Atish , Zaini Nurmahirah binte Mohammed , Poh Zhi Sheng , Wong Brandon Han Siang , Loh Xinpeng , Ng Aik Seng , Low Zun Siong , Prasannan Praseetha , Gong Jade , Tan Michelle Guet Khim , Nagarajan Chandramouli , DaChuan Huang , Lu Pang Wang , Lim Jing Quan , Barrans Sharon , Ong Choon Kiat , LIM Soon Thye , Chng Wee Joo , Follows George , Hodson Daniel , Du Ming-Qing , Goh Yeow Tee , Tan Suat Hoon , Grigoropoulos Nicholas , Verma Navin Kumar 
Published: March 2021

Abstract
Non-Hodgkin’s lymphoma (NHL) is a diverse group of malignancies, encompassing the most common diffuse large B-cell lymphoma (DLBCL) to the rarer T-cell lymphomas. DDX3X is an RNA helicase and, depending on tumour type, plays tumour suppressive or oncogenic roles in cancer. However, its role in NHL is not clear. Targeted sequencing of DDX3X hotspots on exons 8-15 in a cohort of 158 unselected DLBCL subjects showed DDX3X mutations in 5 cases; whereas whole exome sequencing in a cohort of 9 relapsed/refractory DLBCL patients treated with R-CHOP identified DDX3X mutations in 4 cases. DLBCL patients (n=223) with DDX3X mutations had worse 5-year overall survival (22%) compared to patients with wild-type DDX3X (72%, p=0.021). Using DLBCL and cutaneous T-cell lymphoma (CTCL) cell lines, we showed that the expression of mutant DDX3X-R475C or DDX3X knockdown significantly enhanced cell migratory/invasive potential and elevated the phosphorylation of STAT3, Akt and p42/44. DDX3X loss increased resistance to doxorubicin and histone deacetylase targeting drugs in DLBCL and CTCL cells, respectively. Importantly, B- and T-cell lineage DDX3X-depleted cells remained sensitive to STAT3 inhibition. We conclude that DDX3X mutations are important driver lesions in NHL subtypes and are associated with chemoresistance but may be countered with a STAT3 inhibitor.
Effects of Herbaspirillum Sp. P5–19 Assisted With Alien Soil Improvement on the Phytoremediation of Copper Tailings by Vetiveria Zizanioides
Authors: Fang Qing , Huang Tao , Wang Ning , Ding Ziwei , Sun Qingye 
Published: March 2021

Abstract
This study investigated examined the potential of Herbaspirillum sp. p5-19 (p5-19) assisted with alien soil improvement on improving stress tolerance and enhancing the accumulation of Mn, Cu, Zn and Cd by vetiver grass (Vetiveria zizanioides). Phytoremediation potential was evaluated by plant biomass and the ability of plant to absorb and transfer heavy metals. Results showed that the biomass was increased by 19.64%–173.81% in p5-19 inoculation treatments with and without alien soil improvement compared with control. Meanwhile, photosynthetic pigment contents were enhanced in co-inoculation treatment (p5-19 with alien soil improvement). In addition, the malondialdehyde (MDA) content was decreased, and the activities of antioxidant enzymes such as ascorbate peroxidase (APX), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were increased in p5-19 treatment, thereby alleviating the oxidative stress. Moreover, co-inoculation significantly (p<0.05) increased the concentrations of Mn, Cu, Zn and Cd in the roots and shoots of vetiver grass. In particular, the highest concentration of Mn, Zn and Cd in the shoots (roots) were obtained in C10 under p5-19 inoculation (C10B), which were 4.44– (2.71–), 4.73– (3.87–) and 5.93– (4.35–) fold as that of the controls, respectively. These results provided basis for the change of phytoremediation ability of vetiver grass after inoculation. We concluded that p5-19 assisted with alien soil improvement was a potential strategy for enhancing phytoremediation ability in tailings.
Secrecy Performance Analysis of Half/Full Duplex AF/DF Relaying in NOMA Systems Over κ - μ Fading Channels
Authors: Zaghdoud Nesrine , Mnaouer Adel Ben , Boujemaa Hatem , Touati Farid 
Published: March 2021

Abstract
Although the progress in understanding 5G and beyond techniques such as Non-Orthogonal Multiple Access (NOMA) and full-duplex techniques has been overwhelming, still analyzing the security aspects of such systems under different scenarios and settings is an important concern that needs further exploration. In particular, when considering fading in wiretap channels and scenarios, achieving secrecy has posed many challenges. In this context, we propose to study the physical layer security (PLS) of cooperative NOMA (C-NOMA) system using the general fading distribution κ - μ. This distribution facilitates mainly the effect of light-of-sight as well as multipath fading. It also includes multiple distributions as special cases like: Rayleigh, Rice, Nakagami-m which help to understand the comportment of C-NOMA systems under different fading parameters. The use of Half-Duplex and Full-Duplex communication is also investigated for both Amplify-and-forward (AF) and Decode-and-Forward (DF) relaying protocols. To characterize the secrecy performance of the proposed C-NOMA systems, closed form expressions of the Secrecy Outage Probability (SOP) and the Strictly Positive Secrcey Capacity (SPSC) metrics for the strong and weak users are given for high signal-to-noise ratio (SNR) due to the intractable nature of the exact expressions. Based on the analytical analysis, numerical and simulation results are given under different network parameters.
Long-Term Sevoflurane Exposure Inhibits The Proliferation, Differentiation, and Homing Potential of Bone Marrow Mesenchymal Stem Cells
Authors: Bai Yunxiao , Wang Peng , Ma Xiaofang , Li Lili , Zhang Chunyan , Shi Wanchao , Liu Xiaozhi , Bai Yaowu , Zhang Dianying 
Published: March 2021

Abstract
Background: Bone marrow mesenchymal stem cells (BMSCs) are widely used in many fields such as wound repair, gene delivery, and microenvironment improvement. In some cases, BMSC transplantation requires long-term anesthesia. However, the effects of anesthetics on the characteristics of BMSCs are poorly understood.Methods: In this study, we examined the effect of sevoflurane, a gas anesthetic drug most commonly used in children, on the proliferation, differentiation, and homing potential of BMSCs.Results: Short-term (6 h) sevoflurane exposure had almost no effect on the proliferation, differentiation, and homing of BMSCs. However, long-term (24 h) sevoflurane exposure inhibited the proliferation of BMSCs, accelerated their differentiation into nerve cells, and inhibited their homing potential to damaged vascular endothelial cells and intact glioma cells.Conclusion: Short-term anesthesia with sevoflurane as the main inducer is safe and harmless to BMSCs, but long-term sevoflurane exposure may reduce their repair potential. Therefore, because of the high proportion of BMSCs in children, the application of long-term anesthesia with sevoflurane should be cautious, or more suitable anesthetic drugs are needed.
Effect of COVID-19 lockdown on noise pollution levels in an Indian city-A case study of Kanpur
Authors: Mishra Anirudh , Das Sanhita , Singh Deepesh , Maurya Akhilesh Kumar 
Published: March 2021

Abstract
Noise pollution is an emerging environmental threat, prolonged exposure of which can cause annoyance, sleep disturbance, hypertension, psychiatric disorders and also hormonal dysfunction. Among all the sources of noise pollution, the noise generated by road vehicle traffic significantly affects the quality of urban environments. Concerning the recent imposition of COVID-19 societal lockdown, this study attempts to investigate the impacts of COVID-19 lockdown on the changes in noise pollution levels before, during and after lockdown phase in different residential, commercial, industrial and silence zones of the city of Kanpur, India. Although a significant reduction in the noise levels was observed during lockdown, except for commercial zone all other monitoring stations had reported sound levels quite higher than the recommended noise limits set by the Central Pollution Control Board (CPCB) of India. Results further indicated that prominent improvements in health benefits could be observed in the lockdown period, much better than the pre-lockdown and unlock phase. Several noise mitigation strategies are also proposed, which may indeed pave the way for devising noise control measures in the local and regional level.
Conserved structures of neural activity in sensorimotor cortex of freely moving rats allow cross-subject decoding
Authors: Melbaum Svenja , Eriksson David , Brox Thomas , Diester Ilka 
Published: March 2021

Abstract
Our knowledge about neuronal activity in the sensorimotor cortex relies primarily on stereotyped movements which are strictly controlled via the experimental settings. It remains unclear how results can be carried over to less constrained behavior, i.e. freely moving subjects. Towards this goal, we developed a self-paced behavioral paradigm which encouraged rats to conduct different types of movements. Via bilateral electrophysiological recordings across the entire sensorimotor cortex and simultaneous paw tracking, we identified behavioral coupling of neurons with lateralization and an anterior-posterior gradient from premotor to primary sensory cortex. The structure of population activity patterns was conserved across animals, in spite of severe undersampling of the total number of neurons and variations of electrode positions across individuals. Via alignments of low-dimensional neural manifolds, we demonstrate cross-subject and cross-session generalization in a decoding task arguing for a conserved neuronal code.
Research on Dem Construction Methods With Effective Integration of Topographic Feature Lines
Authors: Wang Jing , Zhao Mingwei 
Published: March 2021

Abstract
In cities and other human activity areas, the implementation of various ground projects has resulted in significant changes in natural surface morphology, a prominent feature of which is the formation of a variety of discontinuous terrains, such as roads and building basements. In the process of DEM modeling of these landforms, traditional modeling methods produce obvious topographic distortions at topographic prominences, which limits the application depth of DEMs in these areas. To solve these problems, this paper proposes a DEM modeling method to enhance the expression of discontinuous terrain from the perspective of simplicity and convenience for application. The method is based on terrain data such as topographic feature lines, altimetric points, and contour lines. First, parallel feature lines are generated according to a certain distance. Then, vertices are inserted into the topographic feature line and the parallel feature line according to the specified step length, and the known altimetric points are selected from both sides of the original topographic feature line to estimate the height value of the vertices. Finally, by combining the topographic feature line, parallel feature line and other available topographic data for TIN construction, the result can effectively express the special topography of discontinuous terrain. In this study, a region in Nanjing City, Jiangsu Province, China, was selected as the study area to conduct a DEM construction experiment. The experimental results showed that the DEM constructed by this method could well express the morphological characteristics of discontinuous terrain, and the height accuracy of the construction results was also significantly improved compared with that of the conventional method.