bluuebunny/crossref_metadata_2025_split huggingface dataset:
The sooner the better: clinical and neural correlates of impulsive choice in Tourette disorder
Authors: Atkinson-Clement Cyril , de Liege Astrid , Klein Yanica , Beranger Benoit , Valabregue Romain , Delorme Cecile , Roze Emmanuel , Fernandez-Egea Emilio , Hartmann Andreas , Robbins Trevor W. , Worbe Yulia 
Published: November 2021

AbstractReward sensitivity has been suggested as one of the central pathophysiological mechanisms in Tourette disorder. However, the subjective valuation of a reward by introduction of delay has received little attention in Tourette disorder, even though it has been suggested as a trans-diagnostic feature of numerous neuropsychiatric disorders. We aimed to assess delay discounting in Tourette disorder and to identify its brain functional correlates. We evaluated delayed discounting and its brain functional correlates in a large group of 54 Tourette disorder patients and 31 healthy controls using a data-driven approach. We identified a subgroup of 29 patients with steeper reward discounting, characterised by a higher burden of impulse-control disorders and a higher level of general impulsivity compared to patients with normal behavioural performance or to controls. Reward discounting was underpinned by resting-state activity of a network comprising the orbito-frontal, cingulate, pre-supplementary motor area, temporal and insular cortices, as well as ventral striatum and hippocampus. Within this network, (i) lower connectivity of pre-supplementary motor area with ventral striatum predicted a higher impulsivity and a steeper reward discounting and (ii) a greater connectivity of pre-supplementary motor area with anterior insular cortex predicted steeper reward discounting and more severe tics. Overall, our results highlight the heterogeneity of the delayed reward processing in Tourette disorder, with steeper reward discounting being a marker of burden in impulsivity and impulse control disorders, and the pre-supplementary motor area being a hub region for the delay discounting, impulsivity and tic severity.
RETRACTED ARTICLE: Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs
Authors: Yarmarkovich Mark , Marshall Quinlen F. , Warrington John M. , Premaratne Rasika , Farrel Alvin , Groff David , Li Wei , di Marco Moreno , Runbeck Erin , Truong Hau , Toor Jugmohit S. , Tripathi Sarvind , Nguyen Son , Shen Helena , Noel Tiffany , Church Nicole L. , Weiner Amber , Kendsersky Nathan , Martinez Dan , Weisberg Rebecca , Christie Molly , Eisenlohr Laurence , Bosse Kristopher R. , Dimitrov Dimiter S. , Stevanovic Stefan , Sgourakis Nikolaos G. , Kiefel Ben R. , Maris John M. 
Published: November 2021

AbstractThe majority of oncogenic drivers are intracellular proteins, thus constraining their immunotherapeutic targeting to mutated peptides (neoantigens) presented by individual human leukocyte antigen (HLA) allotypes1. However, most cancers have a modest mutational burden that is insufficient to generate responses using neoantigen-based therapies2,3. Neuroblastoma is a paediatric cancer that harbours few mutations and is instead driven by epigenetically deregulated transcriptional networks4. Here we show that the neuroblastoma immunopeptidome is enriched with peptides derived from proteins that are essential for tumourigenesis and focus on targeting the unmutated peptide QYNPIRTTF, discovered on HLA-A*24:02, which is derived from the neuroblastoma dependency gene and master transcriptional regulator PHOX2B. To target QYNPIRTTF, we developed peptide-centric chimeric antigen receptors (CARs) using a counter-panning strategy with predicted potentially cross-reactive peptides. We further hypothesized that peptide-centric CARs could recognize peptides on additional HLA allotypes when presented in a similar manner. Informed by computational modelling, we showed that PHOX2B peptide-centric CARs also recognize QYNPIRTTF presented by HLA-A*23:01 and the highly divergent HLA-B*14:02. Finally, we demonstrated potent and specific killing of neuroblastoma cells expressing these HLAs in vitro and complete tumour regression in mice. These data suggest that peptide-centric CARs have the potential to vastly expand the pool of immunotherapeutic targets to include non-immunogenic intracellular oncoproteins and widen the population of patients who would benefit from such therapy by breaking conventional HLA restriction.
Terrestrial-type nitrogen-fixing symbiosis between seagrass and a marine bacterium
Authors: Mohr Wiebke , Lehnen Nadine , Ahmerkamp Soeren , Marchant Hannah K. , Graf Jon S. , Tschitschko Bernhard , Yilmaz Pelin , Littmann Sten , Gruber-Vodicka Harald , Leisch Nikolaus , Weber Miriam , Lott Christian , Schubert Carsten J. , Milucka Jana , Kuypers Marcel M. M. 
Published: November 2021

AbstractSymbiotic N2-fixing microorganisms have a crucial role in the assimilation of nitrogen by eukaryotes in nitrogen-limited environments1–3. Particularly among land plants, N2-fixing symbionts occur in a variety of distantly related plant lineages and often involve an intimate association between host and symbiont2,4. Descriptions of such intimate symbioses are lacking for seagrasses, which evolved around 100 million years ago from terrestrial flowering plants that migrated back to the sea5. Here we describe an N2-fixing symbiont, ‘Candidatus Celerinatantimonas neptuna’, that lives inside seagrass root tissue, where it provides ammonia and amino acids to its host in exchange for sugars. As such, this symbiosis is reminiscent of terrestrial N2-fixing plant symbioses. The symbiosis between Ca. C. neptuna and its host Posidonia oceanica enables highly productive seagrass meadows to thrive in the nitrogen-limited Mediterranean Sea. Relatives of Ca. C. neptuna occur worldwide in coastal ecosystems, in which they may form similar symbioses with other seagrasses and saltmarsh plants. Just like N2-fixing microorganisms might have aided the colonization of nitrogen-poor soils by early land plants6, the ancestors of Ca. C. neptuna and its relatives probably enabled flowering plants to invade nitrogen-poor marine habitats, where they formed extremely efficient blue carbon ecosystems7.
Robotic fabrication of high-quality lamellae for aberration-corrected transmission electron microscopy
Authors: Tsurusawa Hideyo , Nakanishi Nobuto , Kawano Kayoko , Chen Yiqiang , Dutka Mikhail , Van Leer Brandon , Mizoguchi Teruyasu 
Published: November 2021

AbstractAberration-corrected scanning transmission electron microscopy (STEM) is widely used for atomic-level imaging of materials but severely requires damage-free and thin samples (lamellae). So far, the preparation of the high-quality lamella from a bulk largely depends on manual processes by a skilled operator. This limits the throughput and repeatability of aberration-corrected STEM experiments. Here, inspired by the recent successes of “robot scientists”, we demonstrate robotic fabrication of high-quality lamellae by focused-ion-beam (FIB) with automation software. First, we show that the robotic FIB can prepare lamellae with a high success rate, where the FIB system automatically controls rough-milling, lift-out, and final-thinning processes. Then, we systematically optimized the FIB parameters of the final-thinning process for single crystal Si. The optimized Si lamellae were evaluated by aberration-corrected STEM, showing atomic-level images with 55 pm resolution and quantitative repeatability of the spatial resolution and lamella thickness. We also demonstrate robotic fabrication of high-quality lamellae of SrTiO3 and sapphire, suggesting that the robotic FIB system may be applicable for a wide range of materials. The throughput of the robotic fabrication was typically an hour per lamella. Our robotic FIB will pave the way for the operator-free, high-throughput, and repeatable fabrication of the high-quality lamellae for aberration-corrected STEM.
Sitosterol and glucosylceramide cooperative transversal and lateral uneven distribution in plant membranes
Authors: Rondelli V. , Koutsioubas A. , Pršić J. , Deboever E. , Crowet J. M. , Lins L. , Deleu M. 
Published: November 2021

AbstractThe properties of biomembranes depend on the presence, local structure and relative distribution assumed by the thousands of components it is made of. As for animal cells, plant membranes have been demonstrated to be organized in subdomains with different persistence lengths and times. In plant cells, sitosterol has been demonstrated to confer to phospholipid membranes a more ordered structure while among lipids, glycosphingolipids are claimed to form rafts where they tightly pack with sterols. Glucosylceramides are glycosphingolipids involved in plant signalling and are essential for viability of cells and whole plant. The glucosylceramide-sitosterol structural coupling within PLPC membranes is here investigated by Langmuir films, in silico simulations and neutron reflectometry, unveiling that a strong direct interaction between the two molecules exists and governs their lateral and transversal distribution within membrane leaflets. The understanding of the driving forces governing specific molecules clustering and segregation in subdomains, such as glucosylceramide and sitosterol, have an impact on the mechanical properties of biomembranes and could reflect in the other membrane molecules partitioning and activity.
Optimally-weighted non-linear beamformer for conventional focused beam ultrasound imaging systems
Authors: Vayyeti Anudeep , Thittai Arun K. 
Published: November 2021

AbstractA novel non-linear beamforming method, namely, filtered delay optimally-weighted multiply and sum (F-DowMAS) beamforming is reported for conventional focused beamforming (CFB) technique. The performance of F-DowMAS was compared against delay and sum (DAS), filtered delay multiply and sum (F-DMAS), filtered delay weight multiply and sum (F-DwMAS) and filter delay Euclidian weighted multiply and sum (F-DewMAS) methods. Notably, in the proposed method the optimal adaptive weights are computed for each imaging point to compensate for the effects due to spatial variations in beam pattern in CFB technique. F-DowMAS, F-DMAS, and DAS were compared in terms of the resulting image quality metrics, Lateral resolution (LR), axial resolution (AR), contrast ratio (CR) and contrast-to-noise ratio (CNR), estimated from experiments on a commercially available tissue-mimicking phantom. The results demonstrate that F-DowMAS improved the AR by 57.04% and 46.95%, LR by 58.21% and 53.40%, CR by 67.35% and 39.25%, and CNR by 44.04% and 30.57% compared to those obtained using DAS and F-DMAS, respectively. Thus, it can be concluded that the newly proposed F-DowMAS outperforms DAS and F-DMAS. As an aside, we also show that the optimal weighting strategy can be extended to benefit DAS.
Infection induced SARS-CoV-2 seroprevalence and heterogeneity of antibody responses in a general population cohort study in Catalonia Spain
Authors: Karachaliou Marianna , Moncunill Gemma , Espinosa Ana , Castaño-Vinyals Gemma , Jiménez Alfons , Vidal Marta , Santano Rebeca , Barrios Diana , Puyol Laura , Carreras Anna , Mayer Leonie , Rubio Rocío , Cortés Beatriz , Pleguezuelos Vanessa , O’Callaghan-Gordo Cristina , Fossati Serena , Rivas Ioar , Casabonne Delphine , Vrijheid Martine , Izquierdo Luis , Aguilar Ruth , Basagaña Xavier , Garcia-Aymerich Judith , de Cid Rafael , Dobaño Carlota , Kogevinas Manolis 
Published: November 2021

AbstractSparse data exist on the complex natural immunity to SARS-CoV-2 at the population level. We applied a well-validated multiplex serology test in 5000 participants of a general population study in Catalonia in blood samples collected from end June to mid November 2020. Based on responses to fifteen isotype-antigen combinations, we detected a seroprevalence of 18.1% in adults (n = 4740), and modeled extrapolation to the general population of Catalonia indicated a 15.3% seroprevalence. Antibodies persisted up to 9 months after infection. Immune profiling of infected individuals revealed that with increasing severity of infection (asymptomatic, 1–3 symptoms, ≥ 4 symptoms, admitted to hospital/ICU), seroresponses were more robust and rich with a shift towards IgG over IgA and anti-spike over anti-nucleocapsid responses. Among seropositive participants, lower antibody levels were observed for those ≥ 60 years vs < 60 years old and smokers vs non-smokers. Overweight/obese participants vs normal weight had higher antibody levels. Adolescents (13–15 years old) (n = 260) showed a seroprevalence of 11.5%, were less likely to be tested seropositive compared to their parents and had dominant anti-spike rather than anti-nucleocapsid IgG responses. Our study provides an unbiased estimate of SARS-CoV-2 seroprevalence in Catalonia and new evidence on the durability and heterogeneity of post-infection immunity.
Feedback between bottom-up and top-down control of stream biofilm mediated through eutrophication effects on grazer growth
Authors: Iannino Alessandra , Fink Patrick , Weitere Markus 
Published: November 2021

AbstractAlgal biofilms in streams are simultaneously controlled by light and nutrient availability (bottom-up control) and by grazing activity (top-down control). In addition to promoting algal growth, light and nutrients also determine the nutritional quality of algae for grazers. While short-term experiments have shown that grazers increase consumption rates of nutrient-poor algae due to compensatory feeding, nutrient limitation in the long run can constrain grazer growth and hence limit the strength of grazing activity. In this study, we tested the effects of light and phosphorus availability on grazer growth and thus on the long-term control of algal biomass. At the end of the experiment, algal biomass was significantly affected by light, phosphorus and grazing, but the interactive effects of the three factors significantly changed over time. At both high light and phosphorus supply, grazing did not initially reduce algal biomass, but the effect of grazing became stronger in the final three weeks of the experiment. Snail growth was enhanced by light, rather than phosphorus, suggesting that algal quantity rather than quality was the main limiting factor for grazer growth. Our results highlight the role of feedback effects and the importance of long-term experiments in the study of foodweb interactions.
National cohort and meteorological data based nested case–control study on the association between air pollution exposure and thyroid cancer
Authors: Park Sung Joon , Min Chanyang , Yoo Dae Myoung , Choi Hyo Geun 
Published: November 2021

AbstractThe objective of this study was to evaluate the influence of exposure to meteorological conditions, including air pollution, on thyroid cancer. A nested case–control study was conducted utilizing 4632 patients with thyroid cancer and 18,528 control subjects who were matched at a 1:4 ratio by age group, sex, income, and region of residence. Korean National Health Insurance Service-Health Screening Cohort data from 2002 to 2015 were used. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated for thyroid cancer correlated with meteorological and air pollution exposure over a moving average of 3 years before the index dates. For all participants, the adjusted ORs associated with relative humidity (1.01, 95% CI 1.00–1.03, P value = 0.023), ambient atmospheric pressure (1.02, 95% CI 1.01–1.03, P value < 0.001), and sunshine duration (1.17, 95% CI 1.04–1.31, P value = 0.007) indicated correlations with the occurrence of thyroid cancer; however, these results were inconsistent in the subgroup analyses. Overall, exposure to nitrogen dioxide (NO2) (1.33, 95% CI 1.24–1.43, P value < 0.001) and particulate matter (PM10) (0.64, 95% CI 0.60–0.69, P value < 0.001) were related to thyroid cancer. These relationships persisted in the subgroup analyses. In conclusion, thyroid cancer occurrence was positively associated with NO2 exposure and negatively associated with PM10 exposure.
SARS-CoV-2 IgG seropositivity in a cohort of 449 non-hospitalized individuals during Spanish COVID-19 lockdown
Authors: Martínez Patricia Torres , García Paula Diaque , Salas María Rubio , Sánchez Raquel Rodríguez , Avendaño-Ortíz José , Guerrero-Monjo Sandra , García Felipe , Llamas Miguel Ángel , López-Collazo Eduardo , Saz-Leal Paula , del Fresno Carlos 
Published: November 2021

AbstractThe Coronavirus Disease of 2019 (COVID-19) pandemic caused by SARS-CoV-2 led the Spanish government to impose a national lockdown in an attempt to control the spread of the infection. Mobility restrictions and the requirement of a medical prescription for serological testing for COVID-19 were included among the control measures. Under this scenario, between April 15th and June 15th, 2020, we performed an observational study including 449 individuals allowed to be tested according to the governmental restrictions, i.e. fulfilling the following prescription requirements: manifestation of COVID-19-compatible symptoms, contact with a confirmed COVID-19 patient, or employment as an essential worker, including health care workers, firefighters and public safety personnel such as police. Importantly, a relevant feature of the studied cohort was that none of the participants had been hospitalized. We analyzed SARS-CoV-2 IgG seropositivity in this specific cohort, uncovering intrinsic features of great demographic interest. The overall rate of IgG seropositivity was 33.69% (95% CI: 29.27–38.21). This frequency was comparable among the different participant occupations. A RT-PCR positive test, contact with a household member previously tested positive and the presence of COVID-19-compatible symptoms were positively associated with IgG + results. Among these symptoms, ageusia/anosmia was positively and independently associated with SARS-CoV-2 IgG seropositivity, while odynophagia was inversely associated. However, fever, ageusia/anosmia and asthenia were the most frequent symptoms described by IgG + subjects. Therefore, our data illustrate how specific cohorts display particular characteristics that should be taken into account when studying population-wide SARS-CoV-2 seroprevalence and key defining symptoms of COVID-19.
Prognostic value of different N1 lymph node zones in pN1M0 non-small cell lung cancer: a systematic review and meta-analysis
Authors: Hu Fengling , Lin Chutong , Chu Hongling , Ren Peng , Wang Jingdi , Ma Shaohua 
Published: November 2021

AbstractThe IASLC lymph node map grouped the lymph node stations into “zones” for prognostic analyses. In the N1 lymph nodes group, N1 nodes are divided into the Hilar/Interlobar zone (N1h) and Peripheral zone (N1p). There is no consensus on the different prognostic values of N1 lymph nodes in N1h and N1p. Therefore, we conducted a systematic review and meta-analysis to assess the survival difference between N1h and N1p in patients of pN1M0 NSCLC. Medline, the Cochrane Library, Embase, and the Web of science were systematically searched to identify relevant studies published up to April 4th, 2020. A retrospective and prospective cohort study comparing N1h versus N1p to the pN1M0 NSCLC was included. Hazard ratios (HRs) for OS were aggregated according to a fixed or random-effect model. Ten publications for 1946 patients of pN1M0 NSCLC were included for the meta-analysis.The 5-year OS was lower for patients with N1h (HR: 1.67, 95% CI 1.44–1.94; P < 0.001). The pooled 5-year OS in N1h and N1p were 40% and 56%, respectively. The patients in pN1M0 NSCLC have different survival according to different N1 lymph node zones involvement: patients with N1p metastasis have a better prognosis than those with N1h metastasis.
Pro-and anti-inflammatory effects of glucocorticoid Fluticasone on ovarian and immune functions in commercial-aged laying hens
Authors: Hatefi Ali , Zare Shahneh Ahmad , Ansari Pirsaraie Zarbakht , Alizadeh Ali Mohammad , Atashnak Mohammad Pouya , Masoudi Reza , Pio Frederic 
Published: November 2021

AbstractOvarian chronic inflammation has been created and extended in the laying hen mainly via increasing laying frequency and microbial infection, especially during the late stage of production period. This study was aimed to evaluate glucocorticoid (GC) Fluticasone as an anti-inflammatory agent on the gene expression of the ovarian pro-and anti-inflammatory mediators (follicular cyclooxygenases COX 1, 2, and cytokines), inflammatory responses of the immune system, ovarian functions (ovulation rate and follicular growths), and hormones in the commercial-aged laying hens. White Leghorn hens aged 92-weeks were used for four weeks to be supplemented by 2 ppm Fluticasone as an optimum dose obtained in a pre-trial base on ovulation rate. As compared to control, Fluticasone resulted in a significant decrease in the mRNA expression of COX-1 and pro-and anti-inflammatory cytokines, and increase in COX-2 mRNA expression and heterophil to lymphocyte ratio (P < 0.001). A significant reduction was observed in the ovulation rate, follicular size (P < 0.001), ovarian hormones, immunoglobulins, body weight, and food consummation (P ≤ 0.05) by administering GC Fluticasone. Although a relative anti-inflammatory improvement was created by Fluticasone in the ovarian condition, the administration of this glucocorticoid resulted in a considerable reduction in ovarian hormones and functions of commercial aged laying hens.
The interactive role of methane beyond a reactant in crude oil upgrading
Authors: Xu Hao , Li Zhaofei , Li Yimeng , Song Hua 
Published: November 2021

AbstractCrude oil upgrading under methane has been reported to be an economically and environmentally promising process, while the advantageous effect of methane beyond a reactant is not fully explained. In this work, the catalytic performances, physicochemical properties and regenerability of used catalysts after crude oil upgrading under methane and nitrogen are investigated byn-butylbenzene model compound studies, catalyst characterizations and density functional theory calculations. Comparing to nitrogen, methane exhibits a protective effect on the charged catalyst despite the limited conversion, leading to better product quality and catalyst stability. This protective effect is attributed to the interaction between methane and catalytic active sites, which mainly occurs in the internal pores of the zeolitic catalyst support, resulting in unique coke distribution and inhibition of metal deposition. The interactive role of methane beyond a reactant, which is previously underestimated, is suggested to be critical for better performances of catalysts in relevant reaction processes.
Making Race Visible in the Speech, Language, and Hearing Sciences: A Critical Discourse Analysis
Authors: Yu Betty , Horton RaMonda , Munson Benjamin , Newkirk-Turner Brandi L. , Johnson Valerie E. , Khamis-Dakwar Reem , Muñoz Maria L. , Hyter Yvette D. 
Published: November 2021

Purpose:The purpose of this critical discourse analytic study is to identify how two key professional standards documents in the Speech, Language and Hearing Sciences field—the Standards for Certification document and the Essential Functions rubric—contribute to the discursive construction of the ideal speech-language pathologist and audiologist, and to examine whether the experiences and needs of people of color are taken into consideration in these documents.Method:Critical discourse analysis was used as both a conceptual and methodological lens for the systematic analysis of the targeted text.Results:The findings show that considerations of race and racism were almost entirely absent from both documents and thus reflected a discourse of race neutrality that is ideologically consistent with color-blind racism. The enactment of racially coded expectations within a construct of race-neutral discourse maintains racial inequities in the speech, language, and hearing sciences professions.Conclusions:The findings highlight the need for the open acknowledgment of racism in our institutional policies and discourses and official and ongoing commitments to concrete and measurable antiracist actions to counteract systemic racism. Recommendations for and examples of antiracist measures are offered.