bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-Dimensional Magnetic Resonance Fingerprinting in Neonates
Authors: Yu Nali , Kim Jee Young , Han Dongyeob , Kim So Young , Lee Hye Mi , Kim Dong-Hyun , Kim Hyun Gi 
Published: June 2021

Objectives
Magnetic resonance fingerprinting (MRF) allows the simultaneous measurement of multiple tissue properties in a single acquisition. Three-dimensional (3D) MRF with high spatial resolution can be used for neonatal brain imaging. The aim of this study is to apply 3D MRF to neonates and show regional differences and maturation in the brain.

Materials and Methods
In this prospective study, 3D MRF using hybrid radial-interleaved acquisition was performed on phantoms and neonates from December 2019 to October 2020. For the reconstruction of 3D MRF, singular value decomposition was applied to reduce reconstruction time, and the iterative reconstruction technique was applied to improve image quality. The accuracies of T1 and T2 values derived from 3D MRF were evaluated in a phantom experiment. Regional T1 and T2 values were obtained from neonates' brain T1 and T2 maps derived from 3D MRF. Regional T1 and T2 values were compared, and their changes according to corrected gestational age were evaluated.

Results
The acquisition time for 3D MRF with a spatial resolution of 0.7 × 0.7 × 2 mm3 was less than 5 minutes. The phantom study showed high correlation between T1 and T2 values derived from 3D MRF and those from conventional spin echo sequences (T1, R
2 = 0.998, P < 0.001; T2, R
2 = 0.998, P < 0.001). Three-dimensional MRF was performed in 25 neonates (15 boys, 10 girls; median corrected gestational age, 263 days; interquartile range, 10 days). In neonates, T1 and T2 values differed in the frontal (median [interquartile range], 2785 [2684–2888] milliseconds and 189.8 [176.7–222.9] milliseconds), parietal (2849 [2741–2950] milliseconds and 191.6 [167.5–232.9] milliseconds), and occipital white matter (2621 [2513–2722] milliseconds and 162.9 [143.5–186.1] milliseconds), showing lower values in occipital white matter (P < 0.001). Regional T1 values showed a negative relationship with corrected gestational age (coefficient, −0.775 to −0.480; P < 0.05).

Conclusions
Fast and high spatial resolution 3D MRF was applied to neonates. T1 and T2 maps derived from 3D MRF enabled the quantification of regional differences and maturation in the neonatal brain.
A Simple Technique to Correct Lumpectomy Deformities Using Free Nipple Grafting
Authors: Van Vliet Austin , Dayicioglu Deniz 
Published: June 2021

Background
Lumpectomy followed by radiation can lead to severe breast asymmetry. Many surgeons are hesitant to perform traditional mastopexies on irradiated breasts due to increased complication rates. An alternative approach to achieve breast symmetry is presented. This technique consists of free nipple-areola complex (NAC) grafting of the irradiated breast to a higher position and primary closure of the donor site, in an appropriate fashion without undermining, followed by a formal mastopexy of the nonradiated breast.

Objective
To evaluate the outcomes of free NAC grafting, used as an alternative method to achieve breast symmetry, in patients who underwent lumpectomy with radiation.

Methods
A case series of 5 patients who underwent breast revision using this technique, performed by a single surgeon from 2017 to 2019 (n = 5), is presented.

Patient Demographics
All patients had history of lumpectomy followed by radiation therapy. The average age was 59.2 years, average BMI was 33.0. Three of 5 patients had a significant smoking history. The average time between radiation and surgery was 5.9 years.

Results
The average operating time was 141.8 minutes. The average follow-up period was 5.8 months. Two (40%) of the free NAC grafts were complicated by hypopigmentation of the reconstructed NAC. No major complications were reported, and no patients required return to the operating room. All patients had successful outcomes with improved breast symmetry.

Conclusions
Free NAC grafting of irradiated breasts and contralateral mastopexy may be a reliable alternative to achieve breast symmetry, with a less invasive approach, in patients who underwent lumpectomy and radiation.
The Case for Onlay Biologic Mesh in Abdominal Wall Reconstruction Using Progressive Tension Suture Fixation
Authors: Lindsey John T. , Boyd Carter J. , Davis Claire , Wilson John , Kurapati Srikanth , de la Torre Jorge I. 
Published: June 2021

Background
After many years of debate, underlay and sublay placement of mesh slowly emerged as the standard of care in abdominal wall reconstruction because of lower hernia recurrence rates. However, onlay has the advantages of being faster, less invasive, and technically easier compared with underlay and sublay. Therefore, if a similar recurrence could be achieved, then onlay should be a consideration. In this study, we present a new onlay method using multipoint progressive tension suture fixation.

Methods
This was a retrospective chart review of patients who underwent abdominal wall reconstruction from 2012 to 2019. Inclusion criteria included onlay mesh placement and at least 1 year of follow-up. The core principles of the surgical technique are establishing myofascial continuity by component separation and reinforcing the repair with onlay mesh that is fixated with multipoint progressive tension sutures.

Results
The number of patients after exclusions was 59, and the average body mass index was 32.52 ± 6.44 kg/m2. More than half (62.7%) of patients had a history of hypertension, 95% had at least 1 prior abdominal/pelvic surgery, and 61% had at least 1 prior hernia repair. Postoperative complications included 20.3% of patients requiring drainage of a fluid collection in the clinic setting, and 29.3% of patients requiring return to the operating room for any reason (including superficial wound debridement). The average defect size was 231.88 ± 195.86 cm2, the mean follow-up was 3.11 ± 1.83 years, and the recurrence rate was 5.1%.

Conclusions
We report a hernia recurrence rate of 5.1% in a high-risk population with complex defects at a mean of 3.1 years of follow-up using onlay mesh fixated with multipoint progressive tension sutures. This recurrence rate is similar to that reported for both underlay and sublay techniques. However, the onlay approach is technically easier, faster, and less invasive compared with underlay and sublay techniques, which may translate into wider reproducibility, lower costs, and improved patient safety.
A Core Outcome Set for Seamless, Standardized Evaluation of Innovative Surgical Procedures and Devices (COHESIVE)
Authors: Avery Kerry N.L. , Wilson Nicholas , Macefield Rhiannon , McNair Angus , Hoffmann Christin , Blazeby Jane M. , Potter Shelley ,   On behalf of the Core Outcomes for early pHasE Surgical Innovation and deVicEs (COHESIVE) study steering group
Published: June 2021

Objective:
To develop a COS, an agreed minimum set of outcomes to measure and report in all studies evaluating the introduction and evaluation of novel surgical techniques.

Summary of Background Data:
Agreement on the key outcomes to measure and report for safe and efficient surgical innovation is lacking, hindering transparency and risking patient harm.

Methods:
(I) Generation of a list of outcome domains from published innovation-specific literature, policy/regulatory body documents, and surgeon interviews; (II) Prioritization of identified outcome domains using an international, multi-stakeholder Delphi survey; (III) Consensus meeting to agree the final COS. Participants were international stakeholders, including patients/public, surgeons, device manufacturers, regulators, trialists, methodologists, and journal editors.

Results:
A total of 7972 verbatim outcomes were identified, categorized into 32 domains, and formatted into survey items/questions. Four hundred ten international participants (220 professionals, 190 patients/public) completed at least one round 1 survey item, of which 153 (69.5%) professionals and 116 (61.1%) patients completed at least one round 2 item. Twelve outcomes were scored “consensus in” (“very important” by ≥70% of patients and professionals) and 20 “no consensus.” A consensus meeting, involving
context: modifications, unexpected disadvantages, device problems, technical procedure completion success, patients’ experience relating to the procedure being innovative, surgeons’/operators’ experience. Other domains relate to intended benefits, whether the overall desired effect was achieved and expected disadvantages.

Conclusions:
The COS is recommended for use in all studies before definitive randomized controlled trial evaluation to promote safe, transparent, and efficient surgical innovation.
SIRT2 inhibition exacerbates p53-mediated ferroptosis in mice following experimental traumatic brain injury
Authors: Gao Jinpeng , Li Yunfeng , Song Ran 
Published: June 2021

Objective
Ferroptosis plays an important role in traumatic brain injury (TBI). The p53 protein is a major mediator of ferroptosis. However, the role of p53-mediated ferroptosis in TBI has not been studied. Sirtuin 2 (SIRT2) exerts a protective effects role in TBI, although the underlying mechanism of this protection remains unclear. In the present study, we tested the hypothesis that that SIRT2 mitigates TBI by regulating p53-mediated ferroptosis.

Methods and results
To model TBI in mice, we used the controlled cortical impact (CCI) injury method. We found that ferroptosis was significantly activated by CCI, and peaked 3 days following CCI, as evidenced by upregulation of GPX4 and SLC7A11, increased content of decreases glutathione, lipid peroxidation, malondialdehyde and ferrous ion. Inhibition of ferroptosis significantly alleviated neurological indications and brain edema. In addition, knockout of p53 significantly blocked ferroptosis following CCI. Furthermore, we found that inhibition of SIRT2 upregulated the acetylation of p53, as well as p53 expression, and exacerbated ferroptosis following CCI. Interestingly, knockout of p53 rescued the SIRT2 inhibition-induced exacerbation of ferroptosis.

Conclusions
These findings indicate that p53-mediated ferroptosis contributes to the pathogenesis of TBI. Furthermore, we demonstrate that SIRT2 exerts a neuroprotective effect against TBI by suppressing p53-mediated ferroptosis.
MicroRNA-488 inhibits neural inflammation and apoptosis in spinal cord injury through restraint on the HMGB1/TLR4/NF-κB signaling pathway
Authors: Niu Feng , Pan Shan 
Published: June 2021

Objectives
Secondary spinal cord injury (SCI), a reversible pathological change, involves neural inflammation and apoptosis. This study explored how microRNA (miR)-488, an inflammatory regulator as reported affected secondary SCI.

Methods
In vivo, Wistar rats were clipped on the spinal cord for SCI induction. In vitro, PC-12 cells were treated with lipopolysaccharide (LPS) to induce cell injuries to mimic the environment during the secondary SCI. Cell viability and apoptosis were measured by CCK-8 assay and flow cytometry. The levels of inflammation-related factors (interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α) in the serum and PC-12 cells were determined by ELISA. The expressions of miR-488, high mobility group box 1 (HMGB1), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3, toll-like receptor 4 (TLR4), phosphorylated (p)-p65 and total-p65 in rat spinal cord or PC-12 cells were analyzed by quantitative reverse transcription PCR or western blot.

Results
After SCI induction, rats exhibited low Basso–Beattie–Bresnahan scores, promoted the release of inflammation-related factors and downregulated miR-488. LPS treatment decreased cell viability, enhanced apoptosis and downregulated miR-488. Upregulating miR-488 neutralized LPS-induced releases of inflammation-related factors and expressions of Bax and cleaved caspase-3 and counteracted LPS-induced inhibition on Bcl-2 expression. MiR-488 directly targeted HMGB1 and miR-488 mimic decreased LPS-induced HMGB1 expression. Overexpressing HMGB1 counteracted miR-488 mimic-induced decreases in the expressions of TLR4 and p-p65 and the ratio of p-p65 to Total-p65 in LPS-treated PC-12 cells.

Conclusion
MiR-488 inhibited neural inflammation and apoptosis in SCI via its binding with HMGB1-mediated restraint on the TLR4/NF-κB signaling pathway.
Ergonomic risk factors and Work-Related Musculoskeletal Disorders among Fireworks workers in West Bengal, India: A cross sectional study
Authors: Laskar Payel , Ganguly Subhadeep , Md Hossain Zakir 
Published: June 2021

ABSTRACTObjectiveFireworks industries are very old, unorganized cottage industries in West Bengal mainly confined in South 24 Parganas. The present investigation was intended to investigate the prevalence of work-related musculoskeletal disorders among the workers and to identify the causative factors behind it.MethodIn this present study, 152 male fireworks workers from different age groups and 100 control subjects were investigated. Modified Nordic questionnaire were used to identify the region wise disorders. Hand Grip strength of both groups were also estimated.ResultAmong the fireworks workers posture related musculoskeletal disorders were severely observed in the lower back which was aggravated with the advancement of age and working experience. Pain and stiffness were also reported in neck, upper back, wrist, elbow, knee and ankle. Lower back rest with support at lumber region was strongly recommended.ConclusionAfter analysis of ergonomic factors and results, it can be concluded that the fire workers who are working with awkward postures have high risk of developing WMSDs specially affecting the upper limbs and both upper and lower back.
HistoClean: Open-source Software for Histological Image Pre-processing and Augmentation to Improve Development of Robust Convolutional Neural Networks
Authors: McCombe Kris D. , Craig Stephanie G. , Pulsawatdi Amélie Viratham , Quezada-Marín Javier I. , Hagan Matthew , Rajendran Simon , Humphries Matthew P. , Bingham Victoria , Salto-Tellez Manuel , Gault Richard , James Jacqueline A. 
Published: June 2021

AbstractThe growth of digital pathology over the past decade has opened new research pathways and insights in cancer prediction and prognosis. In particular, there has been a surge in deep learning and computer vision techniques to analyse digital images. Common practice in this area is to use image pre-processing and augmentation to prevent bias and overfitting, creating a more robust deep learning model. Herein we introduce HistoClean; user-friendly, graphical user interface that brings together multiple image processing modules into one easy to use toolkit. In this study, we utilise HistoClean to pre-process images for a simple convolutional neural network used to detect stromal maturity, improving the accuracy of the model at a tile, region of interest, and patient level. HistoClean is free and open-source and can be downloaded from the Github repository here: https://github.com/HistoCleanQUB/HistoClean.
The transcription factor Roc1 is a regulator of cellulose degradation in the wood-decaying mushroom Schizophyllum commune
Authors: Marian Ioana M. , Vonk Peter Jan , Valdes Ivan D. , Barry Kerrie , Bostock Benedict , Carver Akiko , Daum Chris , Lerner Harry , Lipzen Anna , Park Hongjae , Schuller Margo B. P. , Tegelaar Martin , Tritt Andrew , Schmutz Jeremy , Grimwood Jane , Lugones Luis G. , Choi In-Geol , Wösten Han A. B. , Grigoriev Igor V. , Ohm Robin A. 
Published: June 2021

ABSTRACTWood-decaying fungi of the class Agaricomycetes (phylum Basidiomycota) are saprotrophs that break down lignocellulose and play an important role in the nutrient recycling. They secrete a wide range of extracellular plant cell wall degrading enzymes that break down cellulose, hemicellulose and lignin, the main building blocks of plant biomass. Although the production of these enzymes is regulated mainly at the transcriptional level, no activating regulators have been identified in any wood-decaying fungus in the class Agaricomycetes. We studied the regulation of cellulase expression in the wood-decaying fungus Schizophyllum commune. Comparative genomics and transcriptomics on two wild isolates revealed a Zn2Cys6-type transcription factor gene (roc1) that was highly up-regulated during growth on cellulose, when compared to glucose. It is only conserved in the class Agaricomycetes. A roc1 knockout strain showed an inability to grow on medium with cellulose as sole carbon source, and growth on cellobiose and xylan (other components of wood) was inhibited. Growth on non-wood-related carbon sources was not inhibited. Cellulase activity was reduced in the growth medium of the Δroc1 strain. ChIP-Seq identified 1474 binding sites of the Roc1 transcription factor. Promoters of genes involved in lignocellulose degradation were enriched with these binding sites, especially those of LPMO (lytic polysaccharide monooxygenase) CAZymes, indicating that Roc1 directly regulates these genes. A GC-rich motif was identified as the binding site of Roc1, which was confirmed by a functional promoter analysis. Together, Roc1 is a key regulator of cellulose degradation and the first identified in wood-decaying fungi in the phylum Basidiomycota.
Numerical investigation of an anti-icing method on airfoil based on the NSDBD plasma actuator
Authors: Niu Junjie , Sang Weimin , Zhou Feng , Li Dong 
Published: June 2021

Purpose
This paper aims to investigate the anti-icing performance of the nanosecond dielectric barrier discharge (NSDBD) plasma actuator.

Design/methodology/approach
With the Lagrangian approach and the Messinger model, two different ice shapes known as rime and glaze icing are predicted. The air heating in the boundary layer over a flat plate has been simulated using a phenomenological model of the NSDBD plasma. The NSDBD plasma actuators are planted in the leading edge anti-icing area of NACA0012 airfoil. Combining the unsteady Reynolds-averaged Navier–Stokes equations and the phenomenological model, the flow field around the airfoil is simulated and the effects of the peak voltage, the pulse repetition frequency and the direction arrangement of the NSDBD on anti-icing performance are numerically investigated, respectively.

Findings
The agreement between the numerical results and the experimental data indicates that the present method is accurate. The results show that there is hot air covering the anti-icing area. The increase of the peak voltage and pulse frequency improves the anti-icing performance, and the direction arrangement of NSDBD also influences the anti-icing performance.

Originality/value
A numerical strategy is developed combining the icing algorithm with the phenomenological model. The effects of three parameters of NSDBD on anti-icing performance are discussed. The predicted results show that the anti-icing method is effective and may be helpful for the design of the anti-icing system of the unmanned aerial vehicle.
Ground simulation of fuel cell/battery hybrid propulsion system for small unmanned air vehicles
Authors: Keiyinci Sinan , Aydin Kadir 
Published: June 2021

Purpose
The endurance of small unmanned air vehicles (UAVs) is directly associated with the energy density of the propulsion system used. As the batteries commonly used in small UAVs have a relatively low energy density, they are not sufficient for long-term endurance tasks. The purpose of this paper is to offer a solution to increase the endurance of a concept small UAV with combination of different power sources. The design, construction and ground tests of fuel cell-powered hybrid propulsion systems are presented in this paper.

Design/methodology/approach
The power requirements of a concept UAV were calculated according to aerodynamic calculations and then, hybrid propulsion system sources are determined. The hybrid system consists of a 100 W scale proton-exchange membrane (PEM) type fuel cell stack, lithium-polymer battery, solar cells and power management system (PMS). Subsequently, this hybrid power system was integrated with the new design of PMS and then series of ground tests were carried out.

Findings
This experimental study proved that it is theoretically possible to obtain an endurance of around 3 h for concept UAV with the proposed hybrid system.

Practical implications
The research study shows that fuel cell-based hybrid propulsion system with the proposed PMS can be widely used to obtain extended endurance in small UAVs.

Originality/value
A hybrid propulsion system with a novel PMS unit is proposed for small UAVs and the ground tests were implemented.
A framework for stakeholder management ecosystem
Authors: Tarode Sanchal , Shrivastava Sanjeev 
Published: June 2021

PurposeThe purpose of this study is to develop a stakeholder management ecosystem, which is an improved concept of stakeholder management practices implemented in organizations. The approach is to strategically manage, monitor and assess stakeholders' involvement efficiently during the various stages of the project. The paper aims to structure and organize the stakeholder management ecosystem concept, which would enhance working standards by gaining support and healthy interest of stakeholders in the ever-changing and increasing complex business environment.Design/methodology/approachA theoretical framework study is incorporated on the secondary data of stakeholder management, engagement and assessment. The conceptual insights are drawn for the comprehensive framework of 4Ps (project, people, process and promoting participation) to establish a stakeholder management ecosystem.FindingsThe findings expand the understanding and importance of efficient stakeholder management practices through a stakeholder management ecosystem concept. The implementation of efficient practices can exert a significant effect on the project outcome and organizational goals. Thus, these practices should be assessed and altered according to changing situations and dynamics at the various stages of the project.Practical implicationsThe paper contributes to the literature on stakeholder management. First, it holds organizational and managerial implications to efficiently channelize stakeholder resources to maximize the output of the project and the performance of an organization. Second, managing people associated with an organization formally or informally can not only draw their interest, trust and involvement but also can develop further scope and vision of growth and development.Social implicationsThe philosophy behind the concept is social cooperation and value creation. The more the people are engaged with the organization, the more will be the organizational support and well-being in the community as it broadens the pool of people involved, both inside and outside the organization.Originality/valueThe paper advances the practices of stakeholder management and organization management by introducing the ecosystem concept, 4Ps framework and assessment matrix. The ecosystem concept can be used to develop value and explore the potential of each person associated with an organization and further develop a functional relationship. The 4Ps framework is a structured and flexible approach to ease the process of understanding, analyzing, evaluating and involving stakeholders. The assessment matrix supports the evaluation of the incorporated strategy and further decision-making for the project by gauging project performance and stakeholder involvement.