bluuebunny/crossref_metadata_2025_split huggingface dataset:
Economic and Social Rights, Reparations and the Aftermath of Widespread Violence: The African Human Rights System and Beyond
Authors: Torres Felix E 
Published: May 2021

Abstract
This article examines the dual responsibility of state authorities to repair past abuses and guarantee economic and social rights after episodes of widespread violence according to the jurisprudence of African human rights bodies. Two alternative frameworks underlying the practice of African bodies and human rights law more broadly are discussed. The first portrays the state as a threat to the individual, responsible for redressing the consequences of violations in breach of duties to respect and protect rights. The second understands the state as an active guarantor of rights in the aftermath of widespread abuses, responsible for improving the well-being of people affected and not affected by violence. In light of the possibilities and limitations that arise from both approaches in the African context, the article advocates the second.
The Epigenome and Beyond: How Does Non-genetic Inheritance Change Our View of Evolution?
Authors: Brodie Edmund D , Gregory Brian , Lisch Damon , Riddle Nicole C 
Published: May 2021

Synopsis
Evidence from across the tree of life suggests that epigenetic inheritance is more common than previously thought. If epigenetic inheritance is indeed as common as the data suggest, this finding has potentially important implications for evolutionary theory and our understanding of how evolution and adaptation progress. However, we currently lack an understanding of how common various epigenetic inheritance types are, and how they impact phenotypes. In this perspective, we review the open questions that need to be addressed to fully integrate epigenetic inheritance into evolutionary theory and to develop reliable predictive models for phenotypic evolution. We posit that addressing these challenges will require the collaboration of biologists from different disciplines and a focus on the exploration of data and phenomena without preconceived limits on potential mechanisms or outcomes.
Behaviour in a bottom trawl: moving forward to limit catches of Atlantic cod
Authors: Pol M , Eayrs S 
Published: May 2021

Abstract
The iconic Atlantic cod (Gadus morhua Linnaeus, 1758) has inspired a substantial body of fishing-gear research across its geographical range, with recent efforts predominately to reduce catches of this species in fisheries where their populations are fragile. Despite their iconic status and long history of study compared to other species, our understanding of cod behaviour during the capture process in a bottom trawl remains frustratingly limited. Much of our understanding is derived indirectly through catch results, supported to a limited extent by direct observations of cod in situ or held in laboratories. In this paper, we describe four research challenges and directions that we consider critical to advance our knowledge of cod behaviour, and ultimately, to improve the selectivity of bottom trawls to reduce catches of cod. These include the resurrection of behavioural research to directly observe and measure their reaction and sensory capabilities, and improved interpretation of their behaviour in response to a bottom trawl. It is also our view that progress in limiting catches of cod should emphasize stimulating avoidance in advance or at the mouth of an approaching bottom trawl, rather than retrospectively attempting to do so after they have entered the trawl mouth.
Respiratory Viral Infections and Infection Prevention Practices Among Women With Acute Respiratory Illness During Delivery Hospitalizations During the 2019–2020 Influenza Season
Authors: Dawood Fatimah S , Varner Michael , Munoz Flor , Stockwell Melissa S , Suyama Joe , Li De-Kun , Tita Alan , Mathias Leigh , Shakib Julie H , Piedra Pedro A , Gyamfi-Bannerman Cynthia , Weissman Alexandra , Ferber Jeannette , Battarbee Ashley N , Wesley Meredith G , Vorwaller Kelly , Powers Emily , Gibson Marie , Bond Nanette , Santarcangelo Patricia , Avadhanula Vasanthi , Newes-Adeyi Gabriella , Hunt Danielle Rentz , Subramaniam Akila , Sanusi Ayodeji , Boone Amy , Ogokeh Constance , Macio Ingrid , Odouli Roxana , Thind Priyam , Vargas Celibell Y , Almonte Casandra , Galang Romeo R , Shapiro-Mendoza Carrie , Campbell Angela P 
Published: May 2021

Abstract

Background
We conducted a cross-sectional study of pregnant women with acute respiratory illness during delivery hospitalizations during influenza season to describe clinical testing for respiratory viruses and infection prevention practices.

Methods
Women had nasal swabs tested for influenza and other respiratory viruses. Among 91 enrolled women, 22 (24%) had clinical testing for influenza.

Results
Based on clinical and study testing combined, 41 of 91 (45%) women had samples positive for respiratory viruses. The most common virus was influenza (17 of 91, 19%); 53% (9 of 17) of influenza virus infections were identified through study testing alone. Only 16% of women were on droplet precautions.

Conclusions
Peripartum respiratory infections may be underrecognized.
Ruminal degradation kinetics, intake, digestibility, and feeding behavior of beef steers offered annual or perennial grass-hay with or without supplementation
Authors: Sugg Joel D , Sarturi Jhones O , Henry Darren D , Ciriaco Francine M , West Charles P , Ballou Michael A , Hoffmann Carly A 
Published: May 2021

Abstract
Effects of dried distillers grains plus solubles (DDGS) on ruminal fermentation, degradation kinetics, and feeding behavior of steers offered annual (Eragrostis tef; TEFF) or perennial (Bothriochloa bladhii; OWB) grass hay were evaluated. Ruminally cannulated Angus crossbred steers (n = 6; body weight [BW] = 304 ± 11 kg) were assigned to a 4 × 6 unbalanced Latin square design with four treatments arranged as a 2 × 2 factorial: hay type (OWB or TEFF) and DDGS supplementation (0% or 0.5% BW [dry matter {DM} basis]). Steers had ad libitum access to hay. Periods consisted of a 14-d adaptation followed by 7 d of collection. Residues from the in situ incubations (0, 3, 6, 12, 24, 36, 48, 72, and 96 h post-feeding) were fitted to a first-order kinetics model using the NLIN procedure of SAS. The DDGS decreased (P &lt; 0.01) TEFF DM intake (DMI) by 11.3%, while not affecting DMI of OWB. The greatest DMI was observed for steers supplemented with DDGS, regardless of forage, and least in steers consuming OWB without DDGS (hay type × DDGS; P = 0.03). Non-supplemented steers spent more (P &lt; 0.01) time eating hay. Digestibility of DM tended (P = 0.06) to increase with DDGS supplementation. A hay type × DDGS interaction was observed (P ≤ 0.05) on ruminal effective degradable fractions. The rate of degradation, soluble fraction, and the potentially degradable fraction of organic matter (OM), neutral detergent fiber, and acid detergent fiber (ADF) increased (P ≤ 0.05), while the undegradable fraction of all components decreased (P ≤ 0.01) when steers were offered TEFF compared to OWB. Ruminal DM, OM, and ADF degradation lag-time increased (P ≤ 0.02) in steers offered OWB. Ruminal degradation kinetics were not (P ≥ 0.17) independently affected by DDGS supplementation. Average ruminal pH of steers offered TEFF (P &lt; 0.01) and those offered DDGS (P &lt; 0.01) were lower than OWB and non-supplemented steers. Total concentration of VFA tended (P = 0.09) to increase when DDGS was provided with OWB, while decreasing when TEFF was offered. The acetate:propionate increased (P &lt; 0.01) with DDGS supplementation due to a decrease (P = 0.03) in propionate. Ruminal NH3-N was greater (P = 0.03) in steers offered TEFF compared to OWB, and those supplemented with DDGS (P = 0.03). An annual, in place of a conventional, perennial hay improved intake and digestion of nutrients, without affecting feeding behavior. The supplementation with DDGS appears to affect forage intake, ruminal degradation, and feeding behavior, although not independent of forage quality.
Comparative Efficacy of Commercial Liquid and Mat-Type Electric Vaporizer Insecticides Against Asian Tiger Mosquito (Diptera: Culicidae)
Authors: Jung Hun , An Huijun , Lee Minjin , Lee Jieun , Tak Jun-Hyung 
Published: May 2021

Abstract
Mat and liquid-type electric insecticide vaporizers continuously emit insecticides into the surrounding air. Because both the target insects, mostly mosquitoes, and humans are exposed to those insecticides, it is crucial to understand and monitor their deposition and spatial distribution in treated areas. In the current study, we examined the evaporation of insecticides from seven commercial liquid and mat vaporizers and measured their knock-down and insecticidal activity against the adult females of the Asian tiger mosquito, Aedes albopictus (Skuse). Electric vaporizers from three manufactures had differences in their heaters and active ingredients. Most had continued evaporation during hourly and daily monitoring; however, some liquid vaporizers failed to continue emission to their designated end periods. Overall, mosquitoes located at the upper position in a Peet-Grady chamber and in a field-simulation room exhibited faster knock-down activity than did mosquitoes in other areas, indicating that the insecticides accumulated on the ceiling area. Although most of mat and liquid vaporizers had &lt;60 min of average KT90 values when tested in the Peet-Grady chamber (1.8 × 1.8 × 1.8 m), they failed to have any knock-down in 2 h of observation in the field-simulation room (6.8 × 3.4 × 2.7 m) but showed 72. 8 ± 11.7% and 56. 7 ± 7.3% knock-down in the mat and liquid vaporizers, respectively, in 3 h of operation. Further study will be required to examine whether this relatively limited efficacy can be compensated by other physiological and behavioral effects, including disruption in host-seeking or blood-sucking activities.
First Findings and Molecular Data of Phlebotomus mascittii (Diptera: Psychodidae) in the Cantabrian Cornice (Northern Spain)
Authors: Alarcón-Elbal P M , González M A , Delacour-Estrella S , Bravo-Barriga D , Estrada Peña R , Goiri F , García- Pérez A L , Lucientes J 
Published: May 2021

Abstract
Phlebotomus (Transphlebotomus) mascittii Grassi, 1908 (Diptera: Psychodidae) has been found in several European countries. In Spain, sporadic records were reported in the early ’80s in Catalonia (Northeast Spain), and it was never detected again. Recent entomological surveys carried out between 2004 and 2020 revealed the presence of several specimens of P. mascittii in Spain. The species identification was confirmed by both morphological and molecular analyses. The analyzed specimens belonged to the haplotype (COI_2) defined by one polymorphic site compared to other European specimens. Phlebotomus mascittii was found in low population densities in rural areas associated with livestock farms and in an urban cemetery during the summer season. This study provides the first records of this species in various localities along the Cantabrian cornice (Northern Spain) and represents its westernmost observation in the Palearctic region. The implications of the finding of this uncommon species are discussed at different levels, with emphasis on its suspected role in the transmission of leishmaniosis.
Relationship Between Caregiver Uncertainty, Problem-Solving, and Psychological Adjustment in Pediatric Cancer
Authors: Basile Nathan L , Chardon Marie L , Peugh James , Edwards Clayton S , Szulczewski Lauren , Morrison Caroline F , Nagarajan Rajaram , El-Sheikh Ayman , Chaney John M , Pai Ahna L H , Mullins Larry L 
Published: May 2021

Abstract

Objective
The current study examined the roles of constructive and dysfunctional problem-solving strategies in the relationships between illness uncertainty and adjustment outcomes (i.e., anxious, depressive, and posttraumatic stress symptoms) in caregivers of children newly diagnosed with cancer.

Methods
Two hundred thirty-eight caregivers of children (0–19 years of age) newly diagnosed with cancer (2–14 weeks since diagnosis) completed measures of illness uncertainty, problem-solving strategies, and symptoms of anxiety, depression, and posttraumatic stress.

Results
A mediation model path analysis assessed constructive and dysfunctional problem-solving strategies as mediators between illness uncertainty and caregiver anxious, depressive, and posttraumatic stress symptoms. Dysfunctional problem-solving scores partially mediated the relationships between illness uncertainty and anxious, depressive, and posttraumatic stress symptoms. Constructive problem-solving scores did not mediate these relationships.

Conclusions
The current findings suggest that illness uncertainty and dysfunctional problem-solving strategies, but not constructive problem-solving strategies, may play a key role in the adjustment of caregivers of children newly diagnosed with cancer. Interventions aimed at managing illness uncertainty and mitigating the impact of dysfunctional problem-solving strategies may promote psychological adjustment.
Liposarcoma of the pelvic fossa masquerading as hematoma: a rare case report and its surgical management
Authors: Zarokosta Maria , Foutsitzi Aikaterini , Roditis Spyridon , Karanasiou Vasilissa , Nannou Eirini , Mariolis-Sapsakos Theodoros 
Published: May 2021

Abstract
Liposarcomas constitute rare malignant tumors of the soft tissue, with wide anatomical distribution. The prompt diagnosis of a liposarcoma is extremely challenging since these tumors tend to remain asymptomatic, until they grow enough to displace adjacent anatomical structures. In the presented case, a 55-year-old Caucasian male proceeded to our institution complaining about irreducible swelling of the right iliac fossa and constant discomfort, over the course of a year. His medical history revealed injury of the right groin and pelvis a year ago. The diagnosis was ilioinguinal liposarcoma masquerading as hematoma, due to the previous injury. The patient underwent primary complete tumor resection, and the operation was uneventful. The essential diagnostic and surgical steps for the management of a liposarcoma, mimicking a hematoma are meticulously described.
Regulation of alternative splicing in response to temperature variation in plants
Authors: John Sheeba , Olas Justyna Jadwiga , Mueller-Roeber Bernd 
Published: May 2021

AbstractPlants have evolved numerous molecular strategies to cope with perturbations in environmental temperature, and to adjust growth and physiology to limit the negative effects of extreme temperature. One of the strategies involves alternative splicing of primary transcripts to encode alternative protein products or transcript variants destined for degradation by nonsense-mediated decay. Here, we review how changes in environmental temperature—cold, heat, and moderate alterations in temperature—affect alternative splicing in plants, including crops. We present examples of the mode of action of various temperature-induced splice variants and discuss how these alternative splicing events enable favourable plant responses to altered temperatures. Finally, we point out unanswered questions that should be addressed to fully utilize the endogenous mechanisms in plants to adjust their growth to environmental temperature. We also indicate how this knowledge might be used to enhance crop productivity in the future.
ANT and AIL6: masters of the master regulators during flower development
Authors: Juárez-Corona Ángela G , de Folter Stefan 
Published: May 2021

This article comments on:Krizek BA, Bantle AT, Heflin JM, Han H, Freese NH, Loraine AE. 2021. AINTEGUMENTA and AINTEGUMENTA-LIKE6 directly regulate floral homeotic, growth, and vascular development genes in young Arabidopsis flowers. Journal of Experimental Botany 72, 5478–5493.
Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and diacylglycerol acyltransferase activity
Authors: Xu Yang , Pan Xue , Lu Junhao , Wang Juli , Shan Qiyuan , Stout Jake , Chen Guanqun 
Published: May 2021

Abstract
Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated sub-families of WS, including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally characterized a putative plant-like WS (CzWS1) from the emerging model green microalga Chromochloris zofingiensis. Our results showed that plant-like WS evolved under different selection constraints in plants and microalgae, with positive selection likely contributing to functional divergence. Unlike jojoba with high amounts of wax ester in seeds and a highly active WS enzyme, C. zofingiensis has no detectable wax ester but a high abundance of WS transcripts. Co-expression analysis showed that C. zofingiensis WS has different expression correlation with lipid biosynthetic genes from jojoba, and may have a divergent function. In vitro characterization indicated that CzWS1 had diacylglycerol acyltransferase activity along with WS activity, and overexpression of CzWS1 in yeast and Chlamydomonas reinhardtii affected triacylglycerol accumulation. Moreover, biochemical and bioinformatic analyses revealed the relevance of the C-terminal region of CzWS1 in enzyme function. Taken together, our results indicated a functional divergence of plant-like WS in plants and microalgae, and the importance of its C-terminal region in specialization of enzyme function.
Sphingolipid Δ4-desaturation is an important metabolic step for glycosylceramide formation in Physcomitrium patens
Authors: Gömann Jasmin , Herrfurth Cornelia , Zienkiewicz Krzysztof , Haslam Tegan M , Feussner Ivo 
Published: May 2021

Abstract
Glycosylceramides are abundant membrane components in vascular plants and are associated with cell differentiation, organogenesis, and protein secretion. Long-chain base (LCB) Δ4-desaturation is an important structural feature for metabolic channeling of sphingolipids into glycosylceramide formation in plants and fungi. In Arabidopsis thaliana, LCB Δ4-unsaturated glycosylceramides are restricted to pollen and floral tissue, indicating that LCB Δ4-desaturation has a less important overall physiological role in A. thaliana. In the bryophyte Physcomitrium patens, LCB Δ4-desaturation is a feature of the most abundant glycosylceramides of the gametophyte generation. Metabolic changes in the P. patens null mutants for the sphingolipid Δ4-desaturase (PpSD4D) and the glycosylceramide synthase (PpGCS), sd4d-1 and gcs-1, were determined by ultra-performance liquid chromatography coupled with nanoelectrospray ionization and triple quadrupole tandem mass spectrometry analysis. sd4d-1 plants lacked unsaturated LCBs and the most abundant glycosylceramides. gcs-1 plants lacked all glycosylceramides and accumulated hydroxyceramides. While sd4d-1 plants mostly resembled wild-type plants, gcs-1 mutants were impaired in growth and development. These results indicate that LCB Δ4-desaturation is a prerequisite for the formation of the most abundant glycosylceramides in P. patens. However, loss of unsaturated LCBs does not affect plant viability, while blockage of glycosylceramide synthesis in gcs-1 plants causes severe plant growth and development defects.
Confirmation of ξ1 CMa’s ultra-slow rotation: magnetic polarity reversal and a dramatic change in magnetospheric UV emission lines
Authors: Erba C , Shultz M E , Petit V , Fullerton A W , Henrichs H F , Kochukhov O , Rivinius T , Wade G A 
Published: May 2021

ABSTRACT
The magnetic β Cep pulsator ξ1 CMa has the longest rotational period of any known magnetic B-type star. It is also the only magnetic B-type star with magnetospheric emission that is known to be modulated by both rotation and pulsation. We report here the first unambiguous detection of a negative longitudinal magnetic field in ξ1 CMa (〈Bz〉 = −87 ± 2 G in 2019 and 〈Bz〉 = −207 ± 3 G in 2020), as well as the results of ongoing monitoring of the star’s H α variability. We examine evidence for deviation from a purely dipolar topology. We also report a new HST UV spectrum of ξ1 CMa obtained near magnetic null that is consistent with an equatorial view of the magnetosphere, as evidenced by its similarity to the UV spectrum of β Cep obtained near maximum emission. The new UV spectrum of ξ1 CMa provides additional evidence for the extremely long rotation period of this star via comparison to archival data.
Enhanced delivery of peptide-morpholino oligonucleotides with a small molecule to correct splicing defects in the lung
Authors: Dang Yan , van Heusden Catharina , Nickerson Veronica , Chung Felicity , Wang Yang , Quinney Nancy L , Gentzsch Martina , Randell Scott H , Moulton Hong M , Kole Ryszard , Ni Aiguo , Juliano Rudolph L , Kreda Silvia M 
Published: May 2021

Abstract
Pulmonary diseases offer many targets for oligonucleotide therapeutics. However, effective delivery of oligonucleotides to the lung is challenging. For example, splicing mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) affect a significant cohort of Cystic Fibrosis (CF) patients. These individuals could potentially benefit from treatment with splice switching oligonucleotides (SSOs) that can modulate splicing of CFTR and restore its activity. However, previous studies in cell culture used oligonucleotide transfection methods that cannot be safely translated in vivo. In this report, we demonstrate effective correction of a splicing mutation in the lung of a mouse model using SSOs. Moreover, we also demonstrate effective correction of a CFTR splicing mutation in a pre-clinical CF patient-derived cell model. We utilized a highly effective delivery strategy for oligonucleotides by combining peptide-morpholino (PPMO) SSOs with small molecules termed OECs. PPMOs distribute broadly into the lung and other tissues while OECs potentiate the effects of oligonucleotides by releasing them from endosomal entrapment. The combined PPMO plus OEC approach proved to be effective both in CF patient cells and in vivo in the mouse lung and thus may offer a path to the development of novel therapeutics for splicing mutations in CF and other lung diseases.
Structure of the mammalian adenine DNA glycosylase MUTYH: insights into the base excision repair pathway and cancer
Authors: Nakamura Teruya , Okabe Kohtaro , Hirayama Shogo , Chirifu Mami , Ikemizu Shinji , Morioka Hiroshi , Nakabeppu Yusaku , Yamagata Yuriko 
Published: May 2021

Abstract
Mammalian MutY homologue (MUTYH) is an adenine DNA glycosylase that excises adenine inserted opposite 8-oxoguanine (8-oxoG). The inherited variations in human MUTYH gene are known to cause MUTYH-associated polyposis (MAP), which is associated with colorectal cancer. MUTYH is involved in base excision repair (BER) with proliferating cell nuclear antigen (PCNA) in DNA replication, which is unique and critical for effective mutation-avoidance. It is also reported that MUTYH has a Zn-binding motif in a unique interdomain connector (IDC) region, which interacts with Rad9–Rad1–Hus1 complex (9–1–1) in DNA damage response, and with apurinic/apyrimidinic endonuclease 1 (APE1) in BER. However, the structural basis for the BER pathway by MUTYH and its interacting proteins is unclear. Here, we determined the crystal structures of complexes between mouse MUTYH and DNA, and between the C-terminal domain of mouse MUTYH and human PCNA. The structures elucidated the repair mechanism for the A:8-oxoG mispair including DNA replication-coupled repair process involving MUTYH and PCNA. The Zn-binding motif was revealed to comprise one histidine and three cysteine residues. The IDC, including the Zn-binding motif, is exposed on the MUTYH surface, suggesting its interaction modes with 9–1–1 and APE1, respectively. The structure of MUTYH explains how MAP mutations perturb MUTYH function.