bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphorylation of CAP1 Regulates Lung Cancer Proliferation, Migration and Invasion
Authors: Zeng Jie , Li Xuan , Liang Long , Duan Hongxia , Xie Shuanshuan , Wang Changhui 
Published: May 2021

Abstract
Purpose: Cyclase-associated protein 1 (CAP1) is a ubiquitous protein which regulates actin dynamics. Comprehensive bioinformatics analysis showed CAP1 is a type of cancer-related protein. Previous studies have shown that the protein CAP1 can be phosphorylated. S308 and S310 are the two major phosphorylated sites in human CAP1. In the present study, we aimed to investigate the mechanism of lung cancer metastasis mediated by CAP1. Methods: Massive bioinformatics analysis were applied to determine CAP1’s role in different cancers and especially in lung cancer. Lung cancer patients’ serum was collected and analyzed. CAP1 shRNA-lentivirus were applied to CAP1 gene knockdown, and plasmids were constructed for CAP1 phosphorylation and dephosphorylation. Microarray analysis was used for CAP1-associated difference analysis. Both in vitro and in vivo experiments were performed to investigate the roles of CAP1 phosphorylation and dephosphorylation in lung cancer A549 cells. Results: CAP1 is a kind of cancer related protein. Its mRNA was overexpressed in most types of cancer tissues when compared with normal tissues. CAP1 high expression correlated with poor prognosis. Our results showed that serum CAP1 protein concentrations were significantly upregulated in non-small cell lung cancer (NSCLC) patients when compared with the healthy control group. Knockdown CAP1 in A549 cells can inhibit proliferation and migration. It can also lead to an increase ratio of F-actin/G-actin. In addition, phosphorylated S308 and S310 in CAP1 promoted lung cancer cell proliferation, migration and metastasis both in vitro and in vivo. When dephosphorylated these two sites in CAP1 showed the opposite effect. Phosphorylation of CAP1 can promote epithelial–mesenchymal transition (EMT). Conclusion: These findings indicated that phosphorylation sites of CAP1 might be a novel target for lung cancer treatment.
Establishment of a Gunshot Model of Junctional Femoral Artery Hemorrhage in Swine Under Ultrasonic Guidance
Authors: Zhang Hongliang , Guo Chengyu , Wang Junkang , Feng Cong , Li Tanshi 
Published: May 2021

Abstract
Background: In a combat setting, uncontrolled junctional hemorrhage, which occur at the junction of the torso and appendages, constitutes a major source of potentially preventable deaths. A reliable gunshot swine model of junctional femoral artery rupture was established under ultrasonic guidance to accurately simulate field rescue conditions. Methods: Picco monitoring was instrumented for the anesthetized Landrace pigs, and the right femoral artery was located by portable ultrasound. The pistol bullet hit the right femoral artery along the direction of ultrasonic probe, resulting in the artery rupture. After 30 seconds of uncontrolled hemorrhage, ballistic was filled with Combat Gauze to stop bleeding in BT group (n=10). Combat Gauze (QuikClot) was used to stop bleeding when the mean arterial pressure (MAP) decreased by 30% in MD group (n=10). The sham-operated pigs (n=10) underwent the same anesthetic and surgical procedures, but neither shooting nor gauze filling therapy were performed. No liquid treatment was performed in the three groups throughout the experiment. Blood samples were taken 15 min before injury, then 10 min, 30 min, and 60 min after injury to determine hemodynamic, coagulation, and arterial blood gas indexes. Animals were then monitored for 180 minutes and surviving animals were killed. Results: Histologic anatomy indicated that the right femoral artery and vein were completely ruptured in all 20 swine of MD and BT groups. Eighteen pigs had associated proximal femur fractures and the other two had associated midshaft femur fractures. The entrance diameters of wound were 11.4 mm (SD=0.4), and the exits diameters were 26.4 mm (SD=5.8). The blood loss of pre-tamponade (4.97±2.47 mL/kg vs 18.26±3.47 mL/kg, P<0.001), pro-tamponade (4.58±1.49 mL/kg vs 7.20±1.99 mL/kg, P=0.004) and the total amount of bleeding (9.54±3.80 mL/kg vs 25.46±3.68 mL/kg, P<0.001) in MD group were more than those in BT group. There was statistic difference in the PT, PH, LAC of three groups (P=0.005, 0.004, and<0.001, respectively) and in the body temperature, PH, LAC of different time points (all P<0.001). There were differences in body temperature, PH, PT, LAC of MD group compared with BT and SHAM groups 60 minutes after injury (all P<0.0167). The survival time of MD group was shorter than that of BT group (P=0.029). Conclusion: We established a reliable gunshot model of junctional hemorrhage in swine, which had high accuracy for femoral arterial rupture under ultrasonic guidance and provided consistent and reproducible field-simulation conditions. In this junctional hemorrhage model, blood loss of 30 seconds free bleeding did not meet criteria for shock, and MAP decreased by 30% is a better choice to ensure the success of the shock model.Trial registration: Authorized by Institutional Animal Care and Use Committee in China (grant no. SC2019-06-013).
Optimal mean arterial pressure for favorable neurological outcomes in survivors after extracorporeal cardiopulmonary resuscitation
Authors: Lee Yun Im , Ko Ryoung-Eun , Yang Jeong Hoon , Cho Yang Hyun , Ahn Joonghyun , Ryu Jeong-Am 
Published: May 2021

Abstract
Background: This study aimed to evaluate the optimal target of mean arterial pressure (MAP) for favorable neurological outcomes in patients who underwent extracorporeal cardiopulmonary resuscitation (ECPR).Methods: This was a retrospective, single-center, and observational study of adult patients who underwent ECPR during hospitalization between January 2013 and December 2019. The average MAP was defined as the sum of obtained MAPs divided by their obtained numbers of times during 6, 12, 24, 48, 72, and 96 hrs after cardiac arrest, respectively. The primary outcome was the neurological status upon discharge, as assessed by the Cerebral Performance Categories (CPC) scale (range from 1 to 5). Results: Ultimately, 253 patients were analyzed in this study. Among these patients, 144 (56.9%) survived until discharge from the hospital. Of these survivors, 104 patients had favorable neurologic outcomes (CPC 1 or 2). Overall, patients with favorable neurological outcomes tended to have a higher average MAP than those with poor neurological outcomes. Ensemble algorithms for machine learning and multiple logistic regression revealed that age, Glasgow Coma Scale (GCS) on admission, duration of cardiopulmonary resuscitation (CPR) and average MAPs were significantly associated with neurological outcomes. Six models were established based on these variables and observation times. There was no significant difference in the predicted performance between the 6 hrs model and other models. The patients with average MAP around 75 mmHg had the least probability of poor neurologic outcomes in all the models. However, those with average MAPs below 60 mmHg had a high probability of poor neurological outcomes. Also, based on an increase in the average MAP, the risk of poor neurological outcomes tended to increase in patients with an average MAP greater than 75 mmHg.Conclusions: In this study, age, GCS on admission, CPR duration and average MAPs were identified to be associated with neurological outcomes. Especially, it is hypothesized that the average MAP during the initial 6 hrs may be associated with secondary cerebral injury. Therefore, it is theorized that at an early stage, maintaining the survivor’s MAP to about 75 mmHg may be important for neurological recovery after ECPR.
Poly(o-Phenylenediamine)/ Montmorillonite Nanocomposites: A Novel Strategy to Arrest Bacterial Pathogens Infestation
Authors: Verma Anurakshee , Parveen Romana , Shamsi Tooba Naz , Khan Azmat Ali , fatima sadaf , riaz ufana 
Published: May 2021

Abstract
Antibacterial potential of NC were investigated by the broth microdilution and agar well diffusion methods. Further, effective concentration (EC50) and minimum inhibitory concentration (MIC) of the NC were determined. Intercalation and in-situ polymerization of POPD within MMT was successfully obtained using sonochemical technique.The NC was then checked for antimicrobial activity against four bacterial strains and they showed potent antibacterial activity in both broth and on agar plate. Quantitative test in liquid media clearly showed that NC had viable cells reduction ability for testing strains. Results obtained here concluded that the NC can prevent the infestation of various bacterial pathogens effectively.
NEK7 Promotes Gastric Cancer Progression as a Cell Proliferation Regulator
Authors: Li Yike , Zhu Xiaoran , Zhan Yue , Yuan Wenzhen , Jin Wei-lin 
Published: May 2021

Abstract
Background: Gastric cancer is one of the most common malignant tumors of alimentary canal. However, its targeted therapy develops at a slow pace. Thus, exploring the mechanism of gastric cancer cells’ malignant behavior is of crucial importance to its therapeutic method exploitation. Mammalian NIMA-related kinases are considered to play a significant role in cell proliferation. However, NIMA family proteins have not been reported in gastric cancer yet. Methods: Bioinformatics analysis was used to clarify the expression patterns of NEK1-NEK11 and their effects on prognosis. We also analyzed the effect on immune infiltration and related pathways of NEK7. At cell level, EdU assay, cell cycle assay and CCK-8 assay were utilized to clarify the NEK7 effect on gastric cancer cell proliferation. Mice subcutaneous model revealed the regulating effect on gastric cancer cell proliferation of NEK7 in vivo. Results: Bioinformatics analysis revealed that NEK7 upregulates in gastric cancer and is related to poor prognosis. Meanwhile, NEK7 is related to T-stage, which has closely related to cell proliferation. Further analysis showed that NEK7 has correlation with multiple immune cells infiltration as well as related pathways of gastric cancer. Cell experiments indicated the promoting effect of NEK7 to cell proliferation, while lack of NEK7 could result in gastric cancer proliferation inhibition and G1/S arrest. Conclusion: NEK7 exert the regulatory effect during cell proliferation and is closely related to tumor immune infiltration
Antioxidant Status in Relation to Heavy Metals Induced Oxidative Stress in Patients With Polycystic Ovarian Syndrome (PCOS)
Authors: Abudawood Manal , Tabassum Hajera , Alanazi Atheer H. , Almusallam Fatmah , Aljaser Feda , Ali Mir Naiman , Alenzi Naif D. , Alanazi Samyah T. , Alghamdi Manal A. , Altoum Ghadah H. , Alzeer Manar A. , Alotaibi Majed O. , Alnuaim Lulu 
Published: May 2021

Abstract
Polycystic ovary syndrome (PCOS) is a global health concern in women of reproductive age as 1/15th women worldwide, is affected by this syndrome. PCOS is marked by hyperandrogenism, anovulation, menstrual abnormalities, and polycystic ovaries. Metals like arsenic, cadmium, lead and mercury are considered as systemic toxicants/human carcinogens and seems to have devastating effect on the human, even at minimal exposure. One of the probable etiological factor for PCOS identified is oxidative stress. In view of the probable association between oxidative stress, metal toxicity and PCOS, the present study evaluated the role of heavy metal in generation of oxidative stress among females. This prospective study included 106 women (56 women diagnosed with PCOS and 50women who were not diagnosed with PCOS as control women). There was no significant changes in the sociodemographic characteristics between the two groups except with the irregularity in menses and presence of acne. Levels of serum As, Cd, Pb, Hg increased and serum GSH and SOD levels diminished significantly in PCOS group compared to control at P<0.001. SOD was negatively correlated with As& Pb at P<0.05. Additionally, PCOS group exhibited a strong negative correlation between GSH and As (P<0.01), GSH and Pb (p<0.05) and GSH and Hg (P<0.01). Furthermore, As correlated positively with increased levels of Cd, Pb and Hg among PCOS women. A significant positive correlation was determined between Pb & Cd and Cd & Hg at P<0.001. The outcome of the study provides clear insight of the role of metal induced oxidative stress that plays avitalrole in the pathophysiology underlying PCOS and suggestive of the use of these markers as prognostic tools to circumvent the consequences of high risk exposure to these metals among females.
Benefits and challenges of e-learning over approximately a decade
Authors: Yu Zhonggen 
Published: May 2021

Abstract
In the recent decade, e-learning has been immensely accepted and achieved great success in education. This study, aiming to identify the e-learning pedagogy in a recent decade, adopts the narrative literature review method via a comprehensive online database elicitation with English key words “e-learning, technology assisted learning, mobile learning, distance learning, ubiquitous learning, education, teacher”, and “instructor” to retrieve the literature examining e-learning. Through reviewing the publications in the recent decade and main research themes, the study explores the role of information technology literacy in e-learning, e-learning acceptance and effectiveness, e-learning outcomes, interactions in the e-learning context, adaptation to e-learning, motivation and enjoyment, e-learning devices, barriers to e-learning, and ineffectiveness of e-learning alone. Future research directions could focus on specific e-learning situations, e-learning readiness and adaptiviy through interdisciplinary empirical studies.
Role of Cu Layer on the Enhancement of Spin-to- Charge Conversion in Py/Cu/Bi2Se3
Authors: Su Shu Hsuan , Chong Cheong-Wei , Huang Yu-Xiang , Chen Yi-Chun , Marchenkov Vyacheslav Viktorovich , Huang Jung-Chun Andrew 
Published: May 2021

Abstract
The enhancement of spin-to-charge conversion in Py/Cu/Bi2Se3 is achieved when increasing the Cu layer thickness up to 7nm. The conversion rate is studied using spin pumping technique. The inverse IEE length λIEE is found to increase up to ∼2.7nm when 7nm Cu layer is introduced. Interestingly, maximized λIEE is obtained when the effective spin mixing conductance (and thus Js) is decreased due to the Cu insertion. Monotonic increase of λIEE with decreasing Js suggests that IEE relaxation time τ is enhanced due to additional tunnelling barrier (Cu) that limits the interface transmission rate. The results have shown the importance of interface engineering in Py/TI magnetic heterostructure, which is the key factor for optimizing spin-to-charge conversion efficiency.
Vascular Pythiosis Caused by Pythium Aphanidermatum: The First Case Report in Asia
Authors: Thongsuk Pannaporn , Plongla Rongpong , Thammahong Arsa , Tiewsurin Jaruwan , Worasilchai Navaporn , Chindamporn Ariya , Suankratay Chusana 
Published: May 2021

Abstract
Background: Pythium, soil-borne plant pathogens, are in the class Oomycetes. They are not true fungi, but are related to diatom and algae. There are 2 human pathogens including P. insidiosum and P. aphanidermatum. To date, only 1 case of pythiosis caused by P. aphanidermatum has been reported. We present herein the first case of P. aphanidermatum vascular pythiosis in Asia.Case presentation: A 47-year-old Thai woman, living in North Thailand, with ß thalassemia/hemoglobin E presented with acute recurrent arterial insufficiency of both legs. Emergent embolectomy with clot removal was performed. The pathology of the clot exhibited noncaseous granulomatous inflammation with many fungal hyphal elements. PCR identified P. aphanidermatum with 100% identity. Finally diagnosis is vascular pythiosis. Unfortunately, inoperation due to extensive abdominal aorta involvement, the patient eventually expired after treatment with itraconazole, terbinafine, azithromycin, and doxycycline.Conclusions : To date, only 1 case of pythiosis caused by P. aphanidermatum has been reported. We present herein the first case of P. aphanidermatum vascular pythiosis in Asia.
SorGSD: updating and expanding the sorghum genome science database with new contents and tools
Authors: Liu Yuanming , Wang Zhonghuang , Wu Xiaoyuan , Zhu Junwei , Luo Hong , Tian Dongmei , Li Cuiping , Luo Jingchu , Zhao Wenming , Hao Huaiqing , Jing Hai-Chun 
Published: May 2021

Abstract
BackgroundAs the fifth major cereal crop originated from Africa, sorghum (Sorghum bicolor) has become a key C4 model organism for energy plant research. With the development of high-throughput detection technologies for various omics data, much multi-dimensional and multi-omics information has been accumulated for sorghum. Integrating this information may accelerate genetic research and improve molecular breeding for sorghum agronomic traits.ResultsWe updated the Sorghum Genome SNP Database (SorGSD) by adding new data, new features and renamed it to Sorghum Genome Science Database (SorGSD). In comparison with the original version SorGSD, which contains SNPs from 48 sorghum accessions mapped to the reference genome BTx623 (v2.1), the new version was expanded to 289 sorghum lines with both single nucleotide polymorphisms (SNPs) and small insertions/deletions (INDELs), which were aligned to the newly assembled and annotated sorghum genome BTx623 (v3.1). Moreover, phenotypic data and panicle pictures of critical accessions were provided in the new version. We implemented new tools including ID Conversion, Homologue Search and Genome Browser for analysis and updated the general information related to sorghum research, such as online sorghum resources and literature references. In addition, we deployed a new database infrastructure and redesigned a new user interface as one of the Genome Variation Map databases. The new version SorGSD is freely accessible online at https://bigd.big.ac.cn/sorgsd/.ConclusionsSorGSD is a comprehensive integration with large-scale genomic variation, phenotypic information and incorporates online data analysis tools for data mining, genome navigation and analysis. We hope that SorGSD could provide a valuable resource for sorghum researchers to find variations they are interested in and generate customized high-throughput datasets for further analysis.
Impact of the SARS-CoV-2 S-protein G476S mutation on the interaction with human ACE-2 receptor and neutralizing antibodies
Authors: Kwarteng Alexander , Asiedu Ebenezer , Sylverken Augustina Angelina , Larbi Amma , Twumasi Peter 
Published: May 2021

Abstract
The G476S mutation of the SARS-CoV-2 S-protein occurs in the receptor binding domain (RBD), the region that binds to the human angiotensin-converting enzyme 2 (hACE-2) receptor and also the main target for neutralizing antibodies. The 476S variant was first reported in the USA. Emerging evidence show that the 476S variant resists neutralization by antibodies such as S2E12 and CC6.29. The impact of the mutation on the interactions with hACE-2 receptor and the dynamics of the S-protein, has not been not fully explored. Here, we provide insights into the structure dynamics of the 476S variant and investigate the impact of the mutation on interactions with hACE-2 and selected neutralizing antibodies. We report that the mutation induces a destabilization effect in the RBD and an increased flexibility for most of the receptor binding residues. The mutation, however, does not affect the interactions with the hACE-2 receptor. Both Gly-476 and Ser-476, although located within the hACE-2 interacting residue hotspot, do not contribute to the stabilization of the RBD-hACE-2 complex. Our findings suggest that both H014 and P2P-2F6 antibodies neutralize the 476G and 476S S-proteins with similar efficacy.
Kinetics of dehydrogenation of riebeckite Na2Fe23+Fe32+Si8O22(OH)2: An HT-FTIR study
Authors: Ventura Giancarlo Della , Radica Francesco , Galdenzi Federico , Susta Umberto , Cinque Gianfelice , Cestelli-Guidi Mariangela , Mihailova Boriana , Marcelli Augusto 
Published: May 2021

AbstractIn this work, we address the kinetics of dehydrogenation occurring at high temperatures (HT) in riebeckite, a sodic amphibole with the ideal composition Na2Fe23+Fe32+Si8O22(OH)2. We performed isothermal experiments on both powders and single-crystals up to 560 °C and monitored the O-H stretching signal by Fourier transform infrared (FTIR) spectroscopy. Single-crystals show an initial increase in IR absorption intensity due to increasing vibrational amplitudes of the O-H bond stretching, not observed for powders. The OH-intensities vs. time were fitted using the formalism for first-order reactions. The calculated activation energies for H+ diffusion in riebeckite are 159 ± 15 kJ/mol for powders and 216 ± 20 kJ/mol for single crystals, respectively. The exponential factor m in the Avrami-Erofeev equation obtained for crystals ranges between 1.02 and 1.31, suggesting that, unlike powders, the dehydration process in crystals is not a purely first-order reaction. This implies that a second energy barrier must be considered, i.e., diffusion of H+ through the crystal. FTIR imaging showed that H+ diffusion occurs mainly perpendicular to the silicate double-chain. Our results confirm that the release of H+ from riebeckite occurs after the irreversible Fe2+-to-Fe3+ exchange, thus at temperatures &gt;550 °C. To be effective, the process needs the presence of external oxygen that, by interacting with H+ at the crystal surface, triggers the release of H2O molecules. This implies that oxidizing conditions are required for the amphibole to be an efficient water source at depth.
Síndrome de Hamman: una causa rara de dolor torácico en niños
Authors: Pérez Muñoz Andrés Felipe , Salas Mesias Andrea , Pérez Muñoz William Fernando 
Published: May 2021

Introducción: el dolor torácico es una causa frecuente de consulta al servicio de urgencias pediátricas. Una causa poco conocida es el neumomediastino, el cual puede clasificarse en secundario, cuando hay un desencadenante y en espontáneo, cuando no hay causa evidente. También es llamado síndrome de Hamman, el cual tiene una incidencia que oscila entre uno en 30 000 a 44 000 consultas a urgencias y se presenta predominantemente en hombres. Reporte: Presentamos el caso de una niña de 12 años, previamente sana, quien consultó por un cuadro de 12 horas de evolución de dolor torácico de características pleuríticas, de inicio súbito, asociado a disnea y diaforesis, sin historia de trauma, síntomas respiratorios, gastrointestinales u otra sintomatología previa. Y a quien posteriormente se le hizo diagnóstico de neumomediastino espontáneo.
Discusión: esta enfermedad es muy poco frecuente en niños. La mayoría de los casos se autolimitan rápidamente con manejo sintomático y muy pocos pueden complicarse con taponamiento cardiaco. La radiografía de tórax es el método diagnóstico más preciso. .