bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNARE-dependent mitochondria-lysosome contacts regulate glial mitochondrial dynamics and dopaminergic neuron survival
Authors: Wang Honglei , Wang Linfang , Yi Shuanglong , Zhang Shiping , Ho Margaret S. 
Published: August 2021

SummaryMitochondria are dynamic organelles that undergo fission and fusion, enabling swift structural modification to adapt cellular needs. Disturbances in mitochondrial dynamics, frequently defects ascribed to neurons, have been associated with pathological contexts such as neurodegeneration in Parkinson’s disease. Nonetheless, the mechanism of glial mitochondrial dynamics contributing to neurodegeneration remains unclear. Here we present evidence that the Drosophila R-SNARE VAMP7 regulates glial mitochondrial dynamics and dopaminergic neuron survival via modulating the dynamic of mitochondria-lysosome contact, which determines the mitochondrial fission site. Independent of its characterized role in autophagosome-lysosome fusion, glial VAMP7 depletion causes mitochondrial elongation and dysfunction, increased ROS levels, and production of lipid droplets. These conferred changes in glia in turn affects nearby dopaminergic neuron survival. Glial VAMP7 genetically interacts with the mitochondrial fission/fusion factors Drp1 and Marf1 and controls glial mitochondrial dynamics via regulating the frequency and duration of mitochondria-lysosome contact. Our findings indicate that SNARE proteins, although not direct mediators on mitochondrial fusion, provide spatial cues to modulate glial mitochondrial fission via organelle contacts, impacting on neuron survival in a non-cell-autonomous manner.
The clinical utility of two high-throughput 16S rRNA gene sequencing workflows for taxonomic assignment of unidentifiable bacterial pathogens in MALDI-TOF MS
Authors: Lao Hiu-Yin , Ng Timothy Ting-Leung , Wong Ryan Yik-Lam , Wong Celia Sze-Ting , Chan Chloe Toi-Mei , Wong Denise Sze-Hang , Lee Lam-Kwong , Jim Stephanie Hoi-Ching , Leung Jake Siu-Lun , Lo Hazel Wing-Hei , Wong Ivan Tak-Fai , Yau Miranda Chong-Yee , Lam Jimmy Yiu-Wing , Wu Alan Ka-Lun , Siu Gilman Kit-Hang 
Published: August 2021

ABSTRACTBacterial pathogens that cannot be identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are occasionally encountered in clinical laboratories. The 16S rRNA gene is often used for sequence-based analysis to identify these bacterial species. Nevertheless, traditional Sanger sequencing is laborious, time-consuming and low-throughput. Here, we compared two commercially available 16S rRNA gene sequencing tests, which are based on Illumina and Nanopore sequencing technologies, respectively, in their ability to identify the species of 172 clinical isolates that failed to be identified by MALDI-TOF MS. Sequencing data were analyzed by respective built-in analysis programs (MiSeq Reporter Software and Epi2me) and BLAST+ (v2.11.0). Their agreement with Sanger sequencing on species-level identification was determined. Discrepancies were resolved by whole-genome sequencing. The diagnostic accuracy of each workflow was determined using the composite sequencing result as the reference standard. Despite the high base-calling accuracy of Illumina sequencing, we demonstrated that the Nanopore workflow had a comparatively higher taxonomic resolution at the species level. Using built-in analysis algorithms, the concordance of Sanger 16S with the Illumina and Nanopore workflows was 33.14% and 87.79%, respectively. The agreement was 65.70% and 83.14%, respectively, when BLAST+ was used for analysis. Compared with the reference standard, the diagnostic accuracy of optimized Nanopore 16S was 96.36%, which was identical to Sanger 16S and was better than Illumina 16S (71.52%). The turnaround time of the Illumina workflow and the Nanopore workflow was 78h and 8.25h, respectively. The per-sample cost of the Illumina and Nanopore workflows was US$28.5 and US$17.7, respectively.
Spatiotemporally Resolved Multivariate Pattern Analysis for M/EEG
Authors: Higgins Cameron , Vidaurre Diego , Kolling Nils , Liu Yunzhe , Behrens Tim , Woolrich Mark 
Published: August 2021

ABSTRACTAn emerging goal in neuroscience is tracking what information is represented in brain activity over time as a participant completes some task. Whilst EEG and MEG offer millisecond temporal resolution of how activity patterns emerge and evolve, standard decoding methods present significant barriers to interpretability as they obscure the underlying spatial and temporal activity patterns. We instead propose the use of a generative encoding model framework that simultaneously infers the multivariate spatial patterns of activity and the variable timing at which these patterns emerge on individual trials. An encoding model inversion allows predictions to be made about unseen test data in the same way as in standard decoding methodology. These SpatioTemporally Resolved MVPA (STRM) models can be flexibly applied to a wide variety of experimental paradigms, including classification and regression tasks. We show that these models provide insightful maps of the activity driving predictive accuracy metrics; demonstrate behaviourally meaningful variation in the timing of pattern emergence on individual trials; and achieve predictive accuracies that are either equivalent or surpass those achieved by more widely used methods. This provides a new avenue for investigating the brain’s representational dynamics and could ultimately support more flexible experimental designs in future.HIGHLIGHTSWe introduce SpatioTemporally Resolved MVPA (STRM), an approach that explicitly models how successive stages of stimulus processing are distributed in both space and time in M/EEG data.We show that STRM is broadly applicable to diverse types of M/EEG data and outputs meaningful and interpretable maps of how neural representations evolve in space and time at millisecond resolution.The trial-specific deviations in activity pattern timings identified by STRM are not random, but vary systematically with inter-trial differences in behavioural, cognitive and physiological variables.These methods result in predictive accuracy metrics that are mostly equivalent to, or a modest improvement on, conventional methods.
MTORC2 is a physiological hydrophobic motif kinase of S6 Kinase 1
Authors: Majeed Sheikh Tahir , Majeed Rabiya , Malik Aijaz A , Zargar Muhammad Afzal , Andrabi Khurshid Iqbal 
Published: August 2021

AbstractRibosomal protein S6 kinase 1 (S6K1), a major downstream effector molecule of mTORC1, regulates cell growth and proliferation by modulating protein translation and ribosome biogenesis. We have recently identified eIF4E as an intermediate in transducing signals from mTORC1 to S6K1 and further demonstrated that the role of mTORC1 is restricted to inducing eIF4E phosphorylation and interaction with S6K1. This interaction relieves S6K1 auto-inhibition and facilitates its hydrophobic motif (HM) phosphorylation and activation as a consequence. These observations underscore a possible involvement of mTORC1 independent kinase in mediating HM phosphorylation. Here, we report mTORC2 as an in-vivo/physiological HM kinase of S6K1. We show that rapamycin-resistant S6K1 truncation mutant ΔNHΔCT continues to display HM phosphorylation with selective sensitivity toward Torin-1. We also show that HM phosphorylation of wildtype S6K1and ΔNHΔCT depends on the presence of mTORC2 regulatory subunit-rictor. Furthermore, truncation mutagenesis and molecular docking analysis reveal the involvement of a conserved 19 amino acid stretch of S6K1 in mediating interaction with rictor. We finally show that deletion of the 19 amino acid region from wild type S6K1 results in loss of interaction with rictor, with a resultant loss of HM phosphorylation regardless of the presence of functional TOS motif. Our data demonstrate that mTORC2 acts as a physiological HM kinase that can activate S6K1 after its auto-inhibition is overcome by mTORC1. We, therefore, propose a novel mechanism for S6K1 regulation where mTOR complex 1 and 2 act in tandem to activate the enzyme.
The NADPH Oxidase A of Verticillium dahliae is Essential for Pathogenicity, Normal Development, and Stress Tolerance, and it Interacts with Yap1 to Regulate Redox Homeostasis
Authors: Vangalis Vasileios , Papaioannou Ioannis A. , Markakis Emmanouil A. , Knop Michael , Typas Milton A. 
Published: August 2021

AbstractMaintenance of redox homeostasis is vital for aerobic organisms and particularly relevant to plant pathogens. These need to balance between endogenous ROS production that is required for their development and pathogenicity, and host-derived oxidative stress. Endogenous ROS in fungi are generated by the membrane-bound NADPH oxidase (NOX) complexes and the mitochondrial respiratory chain, while the transcription factor Yap1 is a major regulator of the antioxidant response. Here we investigated the roles of NoxA and Yap1 in fundamental biological processes of the important plant pathogen Verticillium dahliae. Deletion of noxA impaired growth and morphogenesis, compromised formation of hyphopodia, diminished penetration ability and pathogenicity, increased sensitivity against antifungal agents, and dysregulated expression of antioxidant genes. On the other hand, deletion of yap1 resulted in defects in conidial and microsclerotia formation, increased sensitivity against oxidative stress, and down-regulated antioxidant genes. Localized accumulation of ROS was observed before conidial fusion and during the heterokaryon incompatibility reaction upon nonself fusion. The frequency of inviable fusions was not affected by deletion of Yap1. Analysis of a double knockout mutant revealed an epistatic relationship between noxA and yap1. Our results collectively reveal instrumental roles of NoxA and ROS homeostasis in the biology of V. dahliae.
Blockchain in the accounting, auditing and accountability fields: a bibliometric and coding analysis
Authors: Secinaro Silvana , Dal Mas Francesca , Brescia Valerio , Calandra Davide 
Published: August 2021

PurposeThis study aims to offer a bibliometric and coding analysis of blockchain articles published in the accounting, auditing and accountability fields.Design/methodology/approachThe data were collected using the Scopus database and a bibliometric and qualitative coding analysis with the keywords “blockchain” and “accounting” or “auditing” or “accountability.” Of the 514 initial sources, 93 peer-reviewed papers, book chapters and conference proceedings in the areas of business, management and accounting were finally selected. Nonscientific sources such as nonpeer-reviewed books and white papers were excluded.FindingsThis study reveals a promising and multidisciplinary field of research dominated by scholars and less by practitioners. Qualitative research, especially discourse analysis, is the most used method among authors. This study gives some useful insights about blockchain's definition and characteristics, business models, processes involved, connection with other technologies and relationships with accounting theories. Among the most interesting insights, the results confirm that technology as an external force can create an intersection among several research areas: accounting, auditing, accountability, business, management, computer science and engineering fields. Finally, in terms of research themes, although blockchain has a clear effect on auditing accounting, the links with the area of accountability are less clear and validated.Originality/valueThis study highlights the current state of the field, combining methodological approaches and providing valuable future research insights. Additionally, it is also a starting point for professionals to fully understand blockchain's characteristics and potential with a constructive and systemic approach.
Food choice drivers of potential lab-grown meat consumers in Australia
Authors: Garcez de Oliveira Padilha Lívia , Malek Lenka , Umberger Wendy J. 
Published: August 2021

PurposeTo examine the market potential for lab-grown meat (LGM) in Australia by: (1) determining consumers' willingness to consume LGM; (2) exploring heterogeneity in both consumers' willingness to consume LGM and food choice values; and (3) characterizing unique consumer clusters (segments) using socio-demographic, behavioral and psychosocial factors.Design/methodology/approachLatent class cluster analysis was conducted using online survey data obtained from a nationally representative sample of 1,078 Australian food shoppers.FindingsSix consumer clusters were identified, each distinct in their degree of willingness to consume LGM and in their food choice values. Three clusters (49% of consumers) indicated some willingness to consume LGM. One segment, “Prospective LGM eaters” (12%), appeared “very willing” to consume LGM. These consumers were more likely to be younger (<35 years); university-educated; have greater prior awareness of LGM; stronger beliefs regarding the potential self- and society-related benefits of growing demand for LGM; and higher trust in diverse information sources.Practical implicationsInsights on the characteristics of each cluster provide useful information for the industry on how to tailor product development and marketing strategies to address the needs of consumers with the greatest potential to consume LGM.Originality/valueThis is the first consumer research on the topic of LGM to explore market opportunities for LGM in Australia using a nationally representative consumer sample.
Re-defining open government data standards for smart cities’ websites: a case study of selected cities
Authors: Lnenicka Martin , Saxena Stuti 
Published: August 2021

Purpose
The purpose of this paper is to study the select Smart Cities of Czech and Indian counterparts and assess the extent to which open government data (OGD) standards are being adhered to using select indicators.

Design/methodology/approach
This study integrates the benchmarking frameworks provided in literature on OGD and apply them to evaluate the OGD standards of the Smart Cities’ websites.

Findings
Whereas the Czech Smart Cities are relatively more advanced in their OGD initiatives, the Indian counterpart is far lagging behind in their endeavors.

Originality/value
While there are many studies on OGD and Smart Cities, there has been no study which seeks to appreciate if the OGD initiatives are being adhered to by the Smart Cities.
Couple stress-based unsymmetric 8-node planar membrane elements with good tolerances to mesh distortion
Authors: Shang Yan , Wu Huanpu 
Published: August 2021

PurposeThe paper aims to propose two new 8-node quadrilateral membrane elements with good distortion tolerance for the modified couple stress elasticity based on the unsymmetric finite element method (FEM).Design/methodology/approachThe nodal rotation degrees of freedom (DOFs) are introduced into the virtual work principle and constrained by the penalty function for approximating the test functions of the physical rotation and curvature. Therefore, only the C0 continuity instead of C1 continuity is required for the displacement during the element construction. The first unsymmetric element assumes the test functions of the displacement and strain using the standard 8-node isoparametric interpolations, while these test functions in the second model are further enhanced by the nodal rotation DOFs. Besides, the trial functions in these two elements are constructed based on the stress functions that cana priorisatisfy related governing equations.FindingsThe benchmark tests show that both the two elements can efficiently simulate the size-dependent plane problems, exhibiting good numerical accuracies and high distortion tolerances. In particular, they can still exactly reproduce the constant couple stress state when the element shape deteriorates severely into the degenerated triangle. Moreover, it can also be observed that the second element model, in which the linked interpolation technique is used, has better performance than the first one, especially in capturing the steep gradients of the physical rotations.Originality/valueAs the proposed new elements use only three DOFs per node, they can be readily incorporated into the existing finite element (FE) programs. Thus, they are of great benefit to analysis of size-dependent membrane behaviors of micro/nano structures.
When the beans got spilled: the tragic case of cafe coffee day
Authors: Jalan Akanksha 
Published: August 2021

Learning outcomes
The learning outcomes of this paper are as follows: to understand the language of accounting, to interpret financial statements to understand beyond what’s reported and to predict the financial health of a company before it is too late.

Case overview/synopsis
The case revolves around the Indian coffee retail giant - Café Coffee Day (hereafter, CCD). Coffee Day Global Limited of which CCD is a part, is the largest producer of Arabica beans in India. The case goes on to discuss the life and profile of VG Siddhartha (hereafter, VGS), whose leadership and farsightedness made coffee a household name in India, traditionally a tea-drinking country. Within just a year or two after its Initial Public Offering in November 2015, the company’s financial and legal troubles began to surface. The worst blow came when VGS, the 60-year-old founder and CEO committed suicide on July 29, 2019. His group’s mounting debt and impending doom had propelled him to take his own life. Today, the future of CCD remains hanging in the balance, with creditors ready and willing to take the firm into bankruptcy.

Complexity academic level
Under-graduates and above.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 1: Accounting and Finance.
Understanding “Riba” (interest): the religious and the rational
Authors: Azmat Saad , Bhatti Ayesha , Hassan M. Kabir 
Published: August 2021

Learning outcomes
The case explores Ayesha’s reasoning, who is also a financial expert, regarding how she approaches the question of Riba (interest) so that she can maximize her financial returns and remain true to her religious identity. The discussion in the case revolves around alternate rationalizations as to why Riba (interest) continues to remain important for many Islamic investors.

Case overview/synopsis
Historically, the prohibition of Riba (interest) prevented the exploitation of the poor borrower who was charged exorbitant interest rates by wealthy lenders. In the modern day, a banking system which operates in a regulated setup and charges market-based interest rates, the rationale regarding the exploitation of the poor seems less compelling. Furthermore, other economic realities such as inflation and currency fluctuations further lend support to protecting one’s investments through prudent financial decisions. In this case the authors approach this decision regarding the prohibition of Riba (interest) in Islam from the point of view of the protagonist, Ayesha Bhatti, who is religiously conscious and is faced with certain personal investment choices.

Complexity academic level
The case focuses on one of the core issues of Islamic finance (IF), that of the prohibition of charging Riba (interest) on debt and the reasons behind this ruling. The relevance of this prohibition to modern day financial markets is essential to understand IF.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 1: Accounting and Finance.
Ultra-high-net-worth individuals: self-presentation and luxury consumption on Instagram
Authors: Michaelidou Nina , Christodoulides George , Presi Caterina 
Published: August 2021

PurposeLimited research has examined the segment of ultra-high-net-worth individuals (UHNWIs). This study aims to explore how this segment self-presents with regard to luxury on Instagram.Design/methodology/approachWe use a qualitative research approach to content-analyze 815 publicly available photos on Instagram. The analytical approach involves multiple stages and yields three key themes.FindingsThrough Instagram images, UHNWIs engage in inconspicuous consumption via subtle displays of luxury possessions and more cues that indicate luxury experiences, power and social connections. The results further identify four dimensions of self-presentation in luxury consumption on social media: ostentatious, humble, revealed and hidden.Research limitations/implicationsThe study adopts an inductive approach to identify themes related to UHNWIs’ self-presentation on Instagram. Other research could adopt a quantitative approach to identify drivers of the various themes. In addition, the unit of analysis was the photo posted by the UHNWI rather than the UHNWI himself or herself. Further research might explicitly consider the overall profile of each UHNWI and their holistic approach to posting with a view to developing a typology of UHNWIs based on the way they self-present and portray their luxury consumption.Practical implicationsLuxury marketers should focus on inconspicuous products and experiences that allow the UHNWIs to decelerate and spend time with their loved ones, access rare experiences and demonstrate power via their networks.Originality/valueWe extend prior research on self-presentation on Instagram. The authors focus on UHNWIs, a neglected yet significant segment of the luxury market.
Semantic enrichment on large scanned collections through their “satellite texts”: the paradigm of Migne’s Patrologia Graeca
Authors: Varthis Evagelos , Tzanavaris Spyros , Giarenis Ilias , Papavlasopoulos Sozon , Drakakis Manolis , Poulos Marios 
Published: August 2021

PurposeThis paper aims to present a methodology for the semantic enrichment on the scanned collection of Migne’s Patrologia Graeca (PG), attempting to easily locate on the Web domain the scanned PG source, when a reference of this source is described and commented on another scanned or textual document, and to semantically enrich PG through related scanned or textual documents named “satellite texts” published by third people. The present enrichment of PG uses as satellite texts the Dorotheos Scholarios's Synoptic Index (DSSI) which act as metadata for PG.Design/methodology/approachThe methodology consists of two parts. The first part addresses the DSSI transcription via a proper web tool. The second part is divided into two subsections: the accomplishment of interlinking the printed column numbers of each scanned PG page with its actual filename, which is the build of a matching function, and the build of a web interface for PG, based on the generated Uniform Resource Identifiers (URIs) of the above first subsection.FindingsThe result of the implemented methodology is a Web portal, capable of providing server-less search of topics with direct (single click) navigation to sources. The produced system is static, scalable, easy to be managed and requires minimal cost to be completed and maintained. The produced data sets of transcribed DSSI and the JavaScript Object Notation (JSON) matching functions are available for personal use of students and scholars under Creative Commons license (CC-BY-NC-SA).Social implicationsScholars or anyone interested in a particular subject can easily locate topics in PG and reference them, using URIs that are easy to remember. This fact contributes significantly to the related scientific dialogue.Originality/valueThe methodology uses the transcribed satellite texts of DSSI, which act as metadata for PG, to semantically enrich PG collection. Furthermore, the built PG Web interface can be used by other satellite texts as a reference basis to further enrich PG, as it provides a direct identification of sources. The presented methodology is general and can be applied to any scanned collection using its own satellite texts.