bluuebunny/crossref_metadata_2025_split huggingface dataset:
p38 MAPK Activity Is Required to Prevent Hyperactivation of NLRP3 Inflammasome
Authors: Shin Jin Na , Rao Lang , Sha Youbao , Abdel Fattah Elmoataz , Hyser Joseph , Eissa N Tony 
Published: June 2021

Abstract
Inflammation contributes to the pathogenesis and morbidity of wide spectrum of human diseases. The inflammatory response must be actively controlled to prevent bystander damage to tissues. Yet, the mechanisms controlling excessive inflammatory responses are poorly understood. NLRP3 inflammasome plays an important role in innate immune response to cellular infection or stress. Its activation must be tightly regulated because uncontrolled inflammasome activation is associated with a number of human diseases. p38 MAPK signaling plays an essential role in the regulation of inflammation. The role of p38 MAPK in inflammatory response associated with the expression of proinflammatory molecules is known. However, the anti-inflammatory functions of p38 MAPK are largely unknown. In this study, we show that pharmacologic inhibition or genetic deficiency of p38 MAPK leads to hyperactivation of NLRP3 inflammasome, resulting in enhanced Caspase 1 activation and IL-1β and IL-18 production. The deficiency of p38 MAPK activity induced an increase of cytosolic Ca2+ and excessive mitochondrial Ca2+ uptake, leading to exacerbation of mitochondrial damage, which was associated with hyperactivation of NLRP3 inflammasome. In addition, mice with deficiency of p38 MAPK in granulocytes had evidence of in vivo hyperactivation of NLRP3 inflammasome and were more susceptible to LPS-induced sepsis compared with wild-type mice. Our results suggest that p38 MAPK negatively regulates NLRP3 inflammasome through control of Ca2+ mobilization. Hyperactivity of inflammasome in p38-deficient mice causes lung inflammation and increased susceptibility to septic shock.
Cutting Edge: Nucleocapsid Vaccine Elicits Spike-Independent SARS-CoV-2 Protective Immunity
Authors: Matchett William E , Joag Vineet , Stolley J Michael , Shepherd Frances K , Quarnstrom Clare F , Mickelson Clayton K , Wijeyesinghe Sathi , Soerens Andrew G , Becker Samuel , Thiede Joshua M , Weyu Eyob , O’Flanagan Stephen D , Walter Jennifer A , Vu Michelle N , Menachery Vineet D , Bold Tyler D , Vezys Vaiva , Jenkins Marc K , Langlois Ryan A , Masopust David 
Published: June 2021

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. Neutralizing Abs target the receptor binding domain of the spike (S) protein, a focus of successful vaccine efforts. Concerns have arisen that S-specific vaccine immunity may fail to neutralize emerging variants. We show that vaccination with a human adenovirus type 5 vector expressing the SARS-CoV-2 nucleocapsid (N) protein can establish protective immunity, defined by reduced weight loss and viral load, in both Syrian hamsters and K18-hACE2 mice. Challenge of vaccinated mice was associated with rapid N-specific T cell recall responses in the respiratory mucosa. This study supports the rationale for including additional viral Ags in SARS-CoV-2 vaccines, even if they are not a target of neutralizing Abs, to broaden epitope coverage and immune effector mechanisms.
The Joint Physician- and Pharmacist-Managed Anticoagulation Clinic is Aiming to Improve Warfarin Management in Outpatients with Atrial Fibrillation in China
Authors: Sha Zimo , Shen Youmei , Zhang Duxiao , Shi Jiaojiao , Wang Zidun , Li Mingfang , Chen Minglong 
Published: June 2021

Background:
Anticoagulation clinic (AC) is aimed at improving anticoagulation control and outcomes in patients with atrial fibrillation (AF). There is little published data on the benefits of providing an AC in China even if Asians have a higher risk of hemorrhage and stroke when taking warfarin. This study attempts to assess the impact of the AC on warfarin management in the Chinese population.

Subjects and Methods:
This prospective observational study was conducted in the First Affiliated Hospital of Nanjing Medical University and consecutively enrolled 42 AF patients from the joint physician-and pharmacist-managed AC from March 2017 to April 2018. The study protocol was approved by the Institutional Review Board of the First Affiliated Hospital of Nanjing Medical University. Patients already on warfarin for at least 6 months before coming to the AC and unwilling to receive AF ablation or switch to non-Vitamin K antagonist oral anticoagulants were included. The anticoagulant activity of warfarin was monitored using the international normalized ratio (INR). The primary outcome of this study was INR control, which was defined by the time-in-therapeutic range (TTR). The TTR before and after visiting the AC was compared.

Results:
The mean age of the enrolled 42 patients was 68.3 ± 10.6 years and 25 (59.5%) were women. The mean CHA2DS2-VASc score and the mean HAS-BLED score were 3.43 ± 1.29 and 1.86 ± 0.80, respectively, in 28 (66.7%) patients with nonvalvular AF. The median follow-up time after visiting the AC was 298.5 days (interquartile range: 151.8–441.8 days). The TTR (% days) before and after visiting the AC was 44.1% ± 27.5% and 69.7% ± 23.3% (P < 0.001), respectively. Meanwhile, 6 (14.3%) and 25 (59.5%) patients had TTR (% days) >65% before and after visiting the AC (P = 0.001), respectively.

Conclusions:
TTR values were higher in AF patients taking warfarin after the AC. More patients reached satisfactory warfarin control after visiting the AC.
Safety and Efficacy of Transvenous Lead Removal after Cardiovascular Implantable Electronic Device Infection in the Older Patients
Authors: Zheng Jia , Gao Yuan , Lan Rongfang , Xu Wei 
Published: June 2021

Background:
Cardiovascular implantable electronic device (CIED) infection is a life-threatening complication following pacemaker therapy. Complete CIED removal is the first-line therapy for patients with CIED system infection. However, the safety and efficacy of transvenous lead removal of infected CIED in older patients (≥75 years of age) are unknown. Thus, our study aimed to investigate the safety and efficacy of transvenous lead removal of infected CIED in older patients.

Subjects and Methods:
This is a single-center retrospective cohort study in which 316 patients with transvenous lead removal after CIED infections were recruited from Nanjing Drum Tower Hospital between January 2013 and October 2019. We evaluated the safety of the CIED removal based on the rate of major complications (e.g., lead perforation, coronary sinus tear, and pericardial tamponade) and minor complications (e.g., minor pericardial effusion, venous thrombus, and minor hematoma). Procedural success rate and infection recurrence were used to evaluate the efficacy. The differences in gender, comorbidities, type of implanted devices, number and type of leads, anesthesia method, extraction strategies, complications, and complete removal rate were also analyzed. The study was approved by the Ethics Committee of Drum Tower Hospital, Nanjing University Medical School (approval No. 2019-230-01) on February 1, 2019.

Results:
In total, 316 patients were included, of whom 115 patients were ≥75 years old, and 79 were male. There were more defibrillation devices in the <75-year-old group (10.9% vs. 4.3%, P = 0.044). Seven cases (6.1%, 7/115) in the ≥75-year-old group had minor complications without major complications, whereas ten cases (5.0%, 10/201, P = 0.796) of <75-year-old group had minor complications with one case had major complications. 111 patients (96.5%, 111/115) in the ≥75-year-old group had complete removal of the pacing system. Four patients (3.5%, 4/115) achieved clinical success without removal failure. 191 patients (95.0%, 191/201, P = 0.933) in the <75-year-old group had complete removal of the pacing system, and nine patients (4.5%, 9/201, P = 0.776) had clinical success with 1 case (0.5%, 1/201) being failed to remove pacing system. Overall, there was no significant difference in the safety and efficacy among the two groups.

Conclusions:
Transvenous lead removal is safe and curative in patients ≥75 years old with CIED infections.
Serum Irisin Levels and its Association with Blood Glucose and Insulin Indices in Diagnosing Insulin Resistance in Adolescents with Polycystic Ovarian Syndrome
Authors: Sharan Bhawina , Sagili Haritha , Kamalanathan Sadish Kumar , Lakshminarayanan Subitha 
Published: June 2021

Context:
Irisin, a new myokine, has been found to be a biological marker of insulin resistance (IR). There is conflicting evidence on serum irisin level in adult women with polycystic ovarian syndrome (PCOS), and data are lacking in adolescents with PCOS.

Aims:
We aimed to evaluate serum Irisin levels and study its association with indices using blood glucose and insulin levels in adolescents with PCOS.

Settings and Design:
This case–control study was conducted in the gynecology outpatient department.

Materials and Methods:
This study was carried out from August 2015 to June 2017. Eighty-two adolescent girls aged 15–19 years were included in the study. Fasting irisin, insulin, blood glucose; 2nd-h insulin (HOMA2-IR), and Quantitative Insulin Sensitivity Check Index (QUICKI) for all participants with addition of 2nd-h blood glucose for cases.

Statistical Analysis:
A correlation between serum irisin level and various biochemical parameters was done using Pearson's correlation.

Results:
Fasting serum irisin was significantly higher among PCOS cases (8.43 [5.84, 13.11]) when compared to controls 4.90 (2.37, 9.09), median (interquartile range) (P = 0.002). Serum irisin was found to have a significant moderate positive correlation with 2nd-h blood glucose, fasting/2nd-h insulin, and HOMA2-IR. A significant moderate negative correlation was observed between irisin and QUICKI.

Conclusions:
Serum irisin levels were significantly elevated in adolescents with PCOS when compared to the controls. A significant correlation of serum irisin with blood glucose and insulin indices indicates that serum irisin could serve as a marker of IR and may help in its diagnosis in adolescents with PCOS.
The Use of Pooled Consecutive Ejaculates in Moderate Male Factor Infertility to Increase Intrauterine Insemination Success
Authors: Gurunath Sumana , Gundlapalli Swathi , Louis John 
Published: June 2021

Background:
Male factor is a predominant cause of infertility. Success rates of intrauterine insemination(IUI) for male factor depend upon minimum semen parameters such as progressive motility > 30%, strict morphology > 4%, total motile count in the native sample (TMSC) >5 million and inseminating motile count (IMC) > 5 million. Couples with parameters lesser than these are usually advised IVF-ICSI. In developing countries, affordability for IVF is an important deterrent to its widespread utilisation.

Aims:
To evaluate pregnancy rates after the use of a pooled consecutive ejaculate in infertile men with semen parameters of < 5 million TMSC, and to compare with pregnancy rates achieved among couples with semen parameters > 5 million TMSC after processing of a single ejaculate.

Settings and Design:
Private infertility practice. Retrospective study from Oct 2012 to June 2019.

Methods and Materials:
All consecutive patients (n=1979) who underwent IUI in the given study period were included. Patients undergoing donor sperm insemination and low IMC (<1 mill) were excluded from the study. The study group (n=128) included patients in whom the total motile sperm count (TMSC) of the native sample was < 5 million. The control group (n=1851) included patients with normal semen parameters. All participants of the study group with TMSC < 5 million were asked to give a second ejaculate within an hour or two of producing the first. The pooled consecutive ejaculates were used for sample processing and insemination. The primary outcome measure was clinical pregnancy rate. The secondary outcome measures were semen quality of the second ejaculate, TMSC and IMC of both groups.

Statistical Analysis:
Quantitative parameters were compared using Independent sample t-test and Mann Whitney u test. Multivariate binary logistic regression analysis was performed to test the association between the explanatory variables and outcome variable. P value < 0.05 was considered statistically significant.

Results:
6.47% (128/1979) of men were required to give a consecutive ejaculate. The initial ejaculate had significantly higher volume (2ml vs 1 ml; P < 0.001); but lower concentration (8 million/ml vs 19 million/ml; P <0.001) and lower progressive motility (25% vs 35%; P <0.001) in comparison with the second. The final IMC of the pooled ejaculate was 9.01 million vs 21.6 million in the control group (P<0.001). The clinical pregnancy rate was comparable between the control group and the consecutive ejaculate group (15.4% vs 15.63%; P =0.94).

Conclusion:
Consecutive semen samples produced immediately after the first have significantly lower volume but higher sperm count and progressive motility. In couples with moderate male factor infertility with semen parameters inadequate for IUI, pooled consecutive ejaculates yield higher inseminating motile counts which enhance IUI success rates.
Cytogenetic Screening in Couples with Recurrent Pregnancy Loss
Authors: Frikha Rim , Turki Fatma , Abdelmoula Nouha , Rebai Tarek 
Published: June 2021

Context:
Recurrent pregnancy loss (RPL) is a devastating reproductive problem that affects more than 2% of couples who are trying to conceive. Chromosomal rearrangements in either carrier are a major cause of clinically recognized abortion.

Aims:
The purpose of this study is to report the prevalence of chromosome abnormalities in RPL and provide clinical characteristics of couples with two and more miscarriages.

Settings and Design:
Genetic counseling in laboratory of histology housed in a Faculty of Medicine of Sfax.

Materials and Methods:
Karyotype was generated from the peripheral blood lymphocyte cultures and the cytogenetic analysis was performed using R-bands after heat denaturation and Giemsa (RHG) banding. A multiplex polymerase chain reaction wherever necessary was done.

Statistical Analysis Used:
SPSS version 17.

Results:
A total of 104 couples with RPL were carried out in this study. The frequency of chromosomal rearrangements was 11.5%, three times more prevalent in men than women (P = 0.08). In addition, the prevalence of chromosomal anomalies increases according to the number of miscarriages (from 4.8% to 7.6%, with 2 or ≥3 miscarriages, respectively; P = 0.9). Finally, a particular familial adverse reproductive background was found in these carriers (P = 0.03).

Conclusions:
These data highlight that an RPL evaluation is appropriate after the second miscarriage and that cytogenetic evaluation is necessary for an accurate approach to elucidate the causes of RPL. Moreover, familial adverse reproductive backgrounds have an impact of being carrier of chromosome abnormalities and a larger study is mandatory to define reproductive characteristics of carriers.
A comprehensive review on formulations containing Abhraka Bhasma
Authors: Jani Krupali , Bedarkar Prashant , Patgiri Biswajyoti 
Published: June 2021

Background:

Abhraka Bhasma, a mineral preparation commonly known in the classical texts of Ayurveda, is one of the most frequently used drugs in Ayurveda. To prepare the Bhasma, selective metallic/mineral origin materials are exposed repeatedly to Puta, which is a traditional method of Bhasma preparation.

Aim:
To prepare comprehensive data related to various formulations, indications, doses, anupan, etc. of Abhraka Bhasma recorded in various Ayurveda texts.

Materials and Methods:
This review comprises data from the Bharat Bhaishjya Ratnaker in a systematic manner related to various aspects of Abhraka Bhasma. Abhraka Bhasma is used as a major and minor ingredient in most of the formulations and a different percentage of Abhraka Bhasma in used in various Kalps of the text. Making a note of this, it has been decided to calculate the percentage of Abhraka Bhasma, anupan, dose, and therapeutic indications in different formulations.

Results:
It is observed that a total of 348 REFERENCES related to various dosages and indications were observed, which includes various types of formulation such as rasa, Vati, gutika, leham, modak, avaleha, loham formulations, etc. In dosage form, Rasa was found the maximum, followed by Vati, Loham, Leham, Gutika, Modak, Parpatti, etc. It is also found that it is used in various disorders such as Arsha (Piles), Jwara (Fever), Prameha (Diabetes), Atisar (Diarrhea), Vaman (Emesis), Hridyaroga (Cardiac disorders), Jirnajwar (Chronic fever), Shola (Inflammation), etc.

Conclusion:
There are 337 formulations that are mentioned in Bharat Bhaishjya Ratnaker, in which Abhraka Bhasma is used as an ingredient and Madhu (Honey) and Godugdha (Cow's Milk) are found to be the most commonly used Anupana (adjuvant/vehicle). About 78% of Rasa formulations have Abhraka Bhasma as an ingredient.
Kebutuhan Heliport Dalam Operasional Helikopter di Bandara Internasional Sam Ratulangi Manado
Authors: Rusman  
Published: June 2021

Bandara Internasional Sam Ratulangi Manado melayani pergerakan pesawat 70-80 per hari, untuk fixed wing dan rotary wing yang terjadwal dan tidak terjadwal. Dalam pelayanan penerbangan memiliki 2 provider, yaitu PT Angkasa Pura I sebagai penyedia fasilitas sisi udara dan Perum LPPNPI sebagai penyedia layanan navigasi udara. Metode penelitian adalah metode analisis deskriptif, dengan menguji teori dan fakta di Bandara Internasional Sam Ratulangi Manado terhadap prosedur dan ketentuan standar dalam dunia penerbangan. Tujuan dari penelitian ini adalah mengkaji permukaan heliport untuk mengambil keselamatan, efisiensi, dan keamanan di Bandara Internasional Sam Ratulangi Manado. Hasil dari penelitian ini adalah kebutuhan heliport untuk operasi helikopter di Bandara Internasional Sam Ratulangi Manado. Dimana heliport berada di permukaan tanah dan dilengkapi dengan marka dan fasilitas pendukung lainnya berdasarkan Lampiran 14 Heliport Volume 2 Aerodrome dan Peraturan Direktur Jenderal Perhubungan Udara Nomor: KP 40 Tahun 2015 Tentang Standar Teknis dan Operasional Peraturan Keselamatan Penerbangan Sipil Bagian 139 Standart CASR 139).
Evaluasi Penyuluhan Kesehatan terhadap Pengetahuan Taruna Remaja Tentang HIV/AIDS
Authors: Sukarman  
Published: June 2021

Pemberian intervensi penyuluhan kesehatan dimaksudkan untuk memperoleh gambaran pengetahuan Taruna Akademi Teknik dan Keselamatan Penerbangan Makassar terhadap konsep dasar HIV/AIDS. Tujuan dari penelitian ini untuk mengetahui pengaruh Pengaruh penyuluhan kesehatan terhadap pengetahuan HIV/AIDS Taruna Akademi Teknik dan Keselamatan Penerbangan Makassar. Penelitian ini merupakan penelitian kuantitatif dengan desain Pre Eksperimen, One group pre-test post-test design. Populasi yang diteliti adalah Taruna Akademi Teknik dan Keselamatan Penerbangan Makassar. Dengan jumlah sampel 165 responden. Teknik penarikan sampel secara purposive sampling. Sebelum pelaksanaan intervensi dilaksanakn Pre test terhadap populasi Taruna Akademi Teknik dan Keselamatan Penerbangan Makassar, setelah itu penarikan sampel diikuti dengan pemberian intervensi penyuluhan tetang konsep dasar HIV/AIDS lalu dievaluasi dengan post test. Instrumen pengumpulan data menggunakan questioner dengan jumlah 22 pertanyaan menggunakan penilaian menurut skala Gutman. Analisa data menggunakan Uji-t berpasangan, dengan tingkat kemaknaan α = 5% (0,05). Hasil analisa data responden dengan Uji-t berpasangan ditemukan bahwa ada Pengaruh penyuluhan kesehatan terhadap pengetahuan HIV/AIDS Taruna Akademi Teknik dan Keselamatan Penerbangan Makassar, dengan nilai P=0,00 (a< 0,05).
Dampak Campuran Bahan Bakar Oktan 92, 95 dan Eco Racing terhadap Kinerja Mesin dan Emisi Gas Buang
Authors: Nur Mulyadi , Erna Wahyu P. Agnes 
Published: June 2021

Politeknik penerbangan Makassar adalah sekolah tinggi dibawah Kementerian Perhubungan Indonesia, dengan tugas pokok melaksanakan pendidikan professional program Diploma bidang keahlian Teknik dan Keselamatan penerbangan. Salah satu program studi pada jurusan Teknik penerbangan adalah Program Studi Teknologi Pemeliharaan Pesawat Udara (TPPU), yang mempelajari seluruh bagian pesawat udara serta merawat dan memperbaiki pesawat udara agar bisa terbang dengan selamat sampai tujuan. Salah satu hambatan yang terjadi pada program studi Teknologi Pemeliharaan Pesawat Udara (TPPU) di Politeknik Penerbangan (POLTEKBANG) Makassar adalah tidak tersedianya bahan bakar yaitu Avgas yang digunakan pada pesawat Piston Enggine sehingga tidak terlaksanya proses pembelajaran atau peraktikum karena tidak adanya dijual bahan bakar di daerah Sulawesi Selatan sehingga harus memesan di Profinsi Jawa Timur Surabaya mengakibatkan biaya semakin tinggi. Kebutuhun bahan bakar gasoline sebagai energy fosil semakin meningkat dan esensial bagi kehidupan sehari-hari, terjadi karena kelangkaan, tidak dapat diperbaharui dan berpolusi merusak lapisan ozon sehingga penting dikakukan penelitian utk mendapatkan kenerja mesin yg optimal dan rendah emisi. Penelitian dilaksanakan agar proses pembelajaran atau Peraktikum dapat berjalan sesuai dengan Kurikulum yang ada pada program studi TPPU POLTEKBANG Makassar. dengan uji ukur bahan bakar murni pada engine dengan kompresi rasio11.3: 1dan memvariasi campuran RON 92, RON 95 dan Eco Recing, pada putaran 4.000 sampai 10.000 Rpm.H asil uji emisi sesuai dengan standart, dapat dijelaskan bahwa hasil terbaik adalah penggunaan bahan bakar oktan 92 dengan CO: 0,52% dan HC: 125 ppm, mampu menghasilkan daya dan torsi optimal dibanding oktan 95. Variasi campuran terbaik adalah perbandingan 90:10 menghasilkan daya maksimal hingga 14,5 Hp dan torsi 12,97 N.m.
ANALISIS BAURAN PEMASARAN KOPI MANDAILING (STUDI KASUS PADA COFFEE SHOP LOPO MANDHELING CAFE, PANYABUNGAN)
Authors: Novebri  , Fadhilah Nurul 
Published: June 2021

Objektif. Penelitian ini bertujuan untuk menganalisis bauran pemasaran kopi khas Mandailing di Lopo Mandheling Cafe yang berlokasi di Panyabungan, Mandailing Natal Sumatera Utara. Adapun beberapa aspek bauran pemasaran yang dianalisis antara lain product (produk), price (harga), place (tempat), dan promotion (promosi/pemasaran).
Material and Metode. Penelitian ini termasuk jenis penelitian kualitatif. Data dikumpulkan melalui observasi, pengamatan lapangan dan wawancara. Data yang telah terkumpul dianalisis dan disusun dalam bentuk laporan hasil penelitian.
Hasil. Dari penelitian yang telah dilakukan diperoleh hasil bahwa aspek-aspek bauran pemasaran kopi Mandailing di Lopo Mandheling Cafe secara keseluruhan bagus, dimana kualitas produk yang sudah terjamin (sudah BPOM dan memiliki sertifikasi halal dari MUI). Harga yang terjangkau di kalangan masyarakat, tempat yang strategis dan nyaman serta promosi produk yang sudah maksimal baik melalui media sosial, maupun promosi langsung ke konsumen.

Kesimpulan. Manajemen dan analisis bauran pemasaran yang baik memberikan pengaruh signifikan terhadap sebuah produk di pasaran. Diharapkan dengan analis bauran pemasaran yang tepat sebuah produk dapat berkembang dengan baik dan memberikan income yang membantu keberlangsungan usaha.