bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Altered Spontaneous Brain Activity Patterns In Herpes Zoster Keratitis Using The Percent Amplitude of Fluctuation Method: A Resting-State Functional Magnetic Resonance Imaging Study
Authors: Liao Xu-Lin , Li Chu-Qi , Ge Qian-Min , Tang Li-Ying , Su Ting , Li Qiu-Yu , Pan Yi-Cong , Shu Hui-Ye , Zhang Li-Juan , Shao Yi 
Published: December 2021

Abstract
Purpose: The purpose of this study was to use the percent amplitude of fluctuation (PerAF) to study the changes in brain activity and nerve function of herpes zoster keratitis (HZK) patients.Methods: We recruited 20 HZK patients and 20 healthy controls (HCs). Each of these groups included ten males and ten females and were matched in weight and age. All participants underwent resting-state functional magnetic resonance imaging (rs-fMRI). The percent amplitude of fluctuation (PerAF) method was used for analysis and detected differences between the two groups in the neurological function of brain areas. We also applied the receiver operating characteristic (ROC) curve to analyze the two groups and did a correlation analysis between the PerAF value, anxiety, and depression score, and visual acuity.Results: The PerAF signal at the right putamen and right precentral gyrus were significantly higher in patients than in HCs. However, the PerAF value of the left inferior temporal was lower in patients than in HCs. In addition, the HZK patients' anxiety and depression score (HADS) and visual acuity (VA) negatively correlated with the PerAF value at the left inferior temporal gyrus. Conclusion: HZK patients had some changes in brain regions, and the changes were also related to their mood and visual acuity. These findings might contribute to other studies in the potential pathological mechanism, disease development, prognosis, and brain function in HZK patients.
The use of a mouthwash and a dentifrice containing antimicrobial phthalocyanine derivative on the reduction of clinical symptoms of COVID-19: A randomized triple-blinded clinical trial
Authors: Poleti Marcelo Lupion , Gregório Danielle , Bistaffa Alisson Gabriel Idelfonso , Fernandes Karen Barros Parron , Vilhena Fabiano Vieira , Santos Paulo Sérgio da Silva , Simão Andréa Name Colado , Lozovoy Marcell Alysson Batisti , Tatibana Berenice Tomoko , Fernandes Thais Maria Freire 
Published: December 2021

Abstract

PurposeThis clinical trial aimed to evaluate the use of a mouthwash and a dentifrice containing antimicrobial phthalocyanine derivative (APD) on the reduction of clinical symptoms in COVID-19 patients.MethodsThis randomized triple-blinded clinical trial enrolled 500 patients aged 18 years or older submitted to COVID-19 test by nasopharyngeal swab RT-qPCR in a reference center for the diagnosis of COVID-19, had no clinical contraindications to perform mouthwash and gargle, and had access to cell phone with communication application. Patients were randomly assigned (1:1) to use a mouthwash, and a dentifrice both containing antimicrobial phthalocyanine derivative (APD Group) or Non-APD (Control Group). All participants were instructed to floss twice a day, toothbrushing during 2 minutes/three times a day, and for gargling/rising (5ml) during 1 minute/three times a day for 7 days. An online questionnaire was sent to collect the clinical symptoms of COVID-19 in three times: T0 (baseline - before using the oral hygiene products); T3 (three days after); and T7 (seven days after). The investigators, patients, and outcomes assessor were blinded to group assignment. Mann-Whitney test, Chi-Square test, Exact Fisher test, and Cochran’s test were used according to the nature of the variables studied, with the level of significance set at P < 0.05.ResultsNo statistically significant difference was found in the prevalence of symptoms between the groups at baseline. A statistically significant reduction of clinical symptoms was found in the Control Group (fatigue, shortness of breath, hoarse voice, sore throat, nasal congestion, and chest pain) and in APD Group (cough, fatigue, shortness of breath, hyposmia/anosmia, dysgeusia, hoarse voice, sore throat, nasal congestion, chest pain, diarrhea and irritability/confusion) during the follow-up period. There were statistically significant differences with a higher symptoms’ prevalence for the Control Group at T3 and T7. Dysgeusia, sore throat, irritability/confusion was less prevalent at the APD Group at T3, and shortness of breath, hyposmia/anosmia, dysgeusia, hoarse voice, sore throat, diarrhea, and irritability/confusion were more prevalent in the Control Group at T7.ConclusionsBased on methodology, the results demonstrated that the regular use of mouthwash and dentifrice containing APD had a positive impact on the clinical symptoms reported by COVID-19 patients.
Cyanidin 3-glucoside Is a Selective Inhibitor of Hepatic Bilirubin Uptake
Authors: Pelizzo Paola , Stebel Marco , Medic Nevenka , Sist Paola , Vanzo Andreja , Anesi Andrea , Vrhovsek Urska , Tramer Federica , Passamonti Sabina 
Published: December 2021

Abstract
Background & Aims: One of the organ-specific functions of the liver is the excretion of bilirubin into the bile. Membrane transport of bilirubin from the blood to the liver is not only an orphan function, as there is no link to the protein/gene entities that carry it out, but also a poorly characterised function. The aim of this study was to investigate the pharmacology of bilirubin uptake in the liver of the female Wistar rat to improve basic knowledge in this neglected area of liver physiology.Methods: We treated isolated, perfused rat livers with repeated single-pass, albumin-free bilirubin boli. We monitored both bilirubin and bilirubin glucuronide in perfusion effluent with a biofluorometric assay. We tested the ability of nine molecules known to be substrates or inhibitors of sinusoidal membrane transporters to inhibit the hepatic uptake of bilirubin.Results: We found that cyanidin 3-glucoside and malvidin 3-glucoside are the only molecules that inhibit bilirubin uptake. These dietary anthocyanins resemble bromosulfophthalein (BSP), a substrate of several sinusoidal membrane transporters. The SLCO-specific substrates estradiol-17 beta-glucuronide, pravastatin, and taurocholate inhibited only bilirubin glucuronide uptake. Cyanidin 3-glucoside and taurocholate acted at physiological concentrations. The SLC22-specific substrates indomethacin and ketoprofen were inactive. We demonstrated the existence of a bilirubin glucuronide transporter that is inhibited by bilirubin, a fact reported only once in the literature.Conclusions: Data indicate that bilirubin and bilirubin glucuronide are transported into the liver via pharmacologically distinct membrane transport pathways. Some dietary anthocyanins may physiologically modulate the uptake of bilirubin into the liver.
Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies
Authors: Qiu Yuan , Chien Chen-Chi , Maroulis Basilis , Gaitas Angelo , Gong Bin 
Published: December 2021

Abstract
In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.
Crowning lithium ions in hole transport layer toward stable perovskite solar cells
Authors: Shen Ying , Deng Kaimo , Chen Qinghua , Gao Gui , li liang 
Published: December 2021

Abstract
State-of-art perovskite solar cells exhibit comparable power conversion efficiency to silicon photovoltaics. However, the device stability remains a major obstacle that restricts widespread application. Doping hole transport layer induced hygroscopicity, ion diffusion, and use of polar solvent are detrimental factors for performance degradation of perovskite solar cells. Here, we report phase transfer catalyzed LiTFSI doping in Spiro-OMeTAD to address these negative impacts. 12-crown-4 as an efficient phase transfer catalyst promotes the dissolution of LiTFSI without requiring acetonitrile. Crowning Li+ ions by forming more stable and less diffusive crown ether-Li+ complexes retards the generation of hygroscopic lithium oxides and mitigates Li+ ion migration. Optimized solar cells deliver enhanced power conversion efficiency and significantly improved stability under humid and thermal conditions compared with the control device. This method can also be applied to dope π-conjugated polymer. Our findings provide a facile avenue to improve the long-term stability of perovskite solar cells.
Gastrointestinal Manifestations of Mature B Cell Neoplasms: Single Center Experiences from the Collective Perspective of Hematology and Pathology
Authors: Demirci Ufuk , Kurt Busem Binboğa , Gülsaran Seda Karaman , Baş Volkan , Kırkızlar Tuğcan Alp , Kırkızlar Hakkı Onur , Ümit Elif Gülsüm , Puyan Fulya Öz , Demir Ahmet Muzaffer 
Published: December 2021

Abstract
Introduction: Hematological malignancies are frequently affecting the gastrointestinal (GI) tract, either by secondary extra nodal or extramedullary extension to the GI tract, or as a primary process arising in the GI tract. Gastrointestinal non-Hodgkin lymphomas (gNHL) appear less than other solid organ tumours in the GI tract. Therefore, in the absence of nodal or extra nodal involvement in imaging methods, there may be difficulties in diagnosis. Haematologists provide more important support by pathologists in terms of diagnosis than imaging methods.Methods: We retrospectively investigated all B-cell lymphoma patients diagnosed in our clinic between 01.01.2015- 01.01.2021. After that, patients not diagnosed by gastrointestinal system sampling were excluded from the study. Demographic data of these patients were obtained from the hospital information system and outpatient clinic files. Slides of these patients were obtained from pathology archive and re-evaluated under light microscope by two pathologists. All cases were diagnosed according to revised World Health Organization (WHO) 2017 classification.Results: 55 patients were diagnosed with B-cell lymphoma by sampling by endoscopy or colonoscopy. 40 of the patients were diagnosed with Diffuse Large B Cell Lymphoma (DLBCL), 10 with Marginal Zone Lymphoma (MZL), 2 with Mantle Cell Lymphoma (MCL), 2 patients with Burkitt Lymphoma (BL) and 1 patient with Lymphoplasmacytic Lymphoma (LPL).Conclusion: Like the literature, the frequency of DLBCL was the highest in patients diagnosed with GI tract in our study. The second most common B-cell lymphoma was MALToma. Although MCL is with a high frequency of GI involvement, there were few patients diagnosed with GI involvement. The reason for this was that the patients were diagnosed with nodal involvement rather than GI sampling.
Analysis of Optimal Harvest and Management of Capture Fishery of Lake Ziway, Central Ethiopia
Authors: Sisay Kindineh 
Published: December 2021

Abstract
Even if there is fluctuation in fish species diversity and relative abundance of the species, Lake Ziway provides numerous environmental and/or ecosystem services. Despite to its importance, the Lake faces numerous threats because of its public good characteristics. Following the exponentially increased demand for fishes and the resource being public good, magnificent number of individuals are participated in capture fisheries in order to secure their livelihood. Free access to the fisheries, illegal fishing gears and environmental degradation are among the main reasons of decline in fish species and degradation of the Lake. From the current stock assessment survey, the study showed decline in biomass of fish over time. These negative trends in the Lake Ziway fisheries and poor water quality led to loss of livelihoods of many households who are directly or indirectly dependent on the Lake. Therefore, management of Lake Ziway fishery becomes very essential to manage the resource efficiently in a way that maximize fishers present and future benefits otherwise, the fish resource will disappear in the near future. Concerning management practices, most of the fishery regulations in this Lake put emphasis on the restriction of fishing gear, but the application of this instrument with its restrictions is almost zero. Moreover, Individual Transferable Quotas (ITQs) foster greater transparency and accountability for the management and enhancement of fishery resources by quota owners. In this regard, since it generates a sense of ownership and its versatility in providing a stable and productive market, ITQs are more effective than gear restrictions and in fact, it has major social benefits by controlling overharvesting. Due to this, to ensure the nutritional and food security of the country currently and in the near future, there is a need to enforce fishing gear restriction and set ITQs. Furthermore, there must be strong effort on studying the current stock of fish, and numbers of legal and illegal fishers to set Individual Transferable Quotas (ITQs) and Total Allowable Catch (TAC) for sustainable fishery management of Lake Ziway.
Structural Economic Vulnerability and the Utilization of Unilateral Trade Preferences: Does Development Aid Matter?
Authors: GNANGNON Sèna Kimm 
Published: December 2021

Abstract
This article has investigated the effect of structural economic vulnerability on the utilization of non-reciprocal trade preferences (NRTPs) offered by the 'Quadrilaterals' and whether development aid flows alter this effect. It considers two major blocks of NRTPs, namely the Generalized System of Preferences (GSP) programs and 'other trade preferences programs'. The analysis uses a panel dataset of 84 beneficiary countries of both NRTPs and development aid, over the period of 2002-2019. Results reveal that a rise in the level of structural economic vulnerability reduces the utilization rates of both GSP programs and other trade preference programs. At the same time, when the level of structural economic vulnerability falls, countries tend to use both blocks of NRTPs in a complementarity way. While development aid inflows foster the utilization of the two blocks of NRTPs, the increase of these resource inflows in the context of greater structural economic vulnerability leads beneficiary countries to strengthen the utilization of other trade preferences programs at the expense of the utilization of GSP programs. The implications of these results are discussed in the conclusion section of the article.
Coexpression Analysis Identified PcMYB25 as a Patchoulol Synthase Gene Activator to Enhance Patchouli Alcohol Biosynthesis in Pogostemon Cablin
Authors: Zhou Xuanxuan , Wang Xilin , Huang Huiling , Wu Daidi , Wang Xiaobing , Zhong Baiyang , Li Junren , Chen Xiuzhen , Zhan Ruoting , Chen Likai 
Published: December 2021

Abstract
BackgroundPatchouli alcohol is an effective component of the medicinal plant patchouli. Similar to other secondary metabolites, its synthesis is likely also regulated by transcription factors. Although the biosynthetic pathway of patchouli alcohol has been characterized, the regulatory mechanism of patchouli alcohol biosynthesis has not been fully revealed.ResultsThis study combined the transcriptome data of patchouli leaves treated with different hormones and WGCNA to establish a coexpression network. The modules correlated to patchouli alcohol content were identified, and PcMYB25 played a crucial role in regulating patchouli alcohol biosynthesis. The overexpression of PcMYB25 can promote the expression of patchouli alcohol synthase (PTS), thereby increasing the content of patchouli alcohol.Conclusions This is the first report that MYB25 regulates the secondary metabolism of patchouli. These experimental results lay the foundation for further analysis of the regulatory mechanism of patchouli alcohol synthesis.
The Effects of Kinesiological Taping and Aerobic Exercise in Women with Primary Dysmenorrhea: A Randomized Single-Blind Controlled Trial
Authors: Temizkan Simge , BUDAK Miray 
Published: December 2021

Abstract
Purpose: The purpose of this study was to investigate the effects of KT and AE on pain, attitude, depression, and quality of life in women with dysmenorrhoea. Methods: Forty-five women with dysmenorrhoea were randomly divided into three groups as KT group(n=15), AE group(n=15) and control group(n=15). In KT Group, 6 sessions of KT were applied, starting on the 14th day of the menstrual cycle and 2 times a week for 3 weeks until the cycle ended. In the AE group, walking and climbing stairs were given during the menstrual cycle, 3 days a week, 45 minutes. No application was made to the control group. All groups were evaluated with Beck Depression Scale(BDS), Mcgill Pain Questionnaire(MPQ), Menstrual Attitude Scale(MAS), and Short Form-36(SF-36) before and after treatment. Results: A significant difference was found in MAS and SF-36 mental and physical in the KT group(p<0.05). There was a significant decrease in the MPQ in the AE group(p<0.05). No significant result was found in the control group. There were significant differences in MPQ and MAS Time*Group interaction(p<0.05). There was no significant difference between the KT and AE Group in post-hoc tests(p<0.05). Conclusion: In conclusion, KT and AE may decrease pain and improve attitude and quality of life in women with dysmenorrhoea. “retrospectively registered” Trial registration number (NCT04856280), date of registration (20.04.2021).
Cultivable Microbial Diversity in Speleothems Using MALDI-TOF Spectrometry and DNA Sequencing from Krem Soitan, Krem Lawbah, Krem Mawpun, Khasi Hills, Meghalaya, India
Authors: Devender Mudgil , Paul Dhiraj , Baskar Sushmitha , Baskar Ramanathan , Shouche Yogesh S 
Published: December 2021

Abstract
This study reports on the culturable microbial communities in caves from the Indian sub-continent. A high bacterial diversity and a greater bacterial taxonomic diversity is reported using MALDI-TOF spectrometry and 16S rRNA gene sequencing. This approach helped to detect a number bacterial strains from the Indian caves. The microbial diversity in the Indian caves is inadequately characterized. The study aims to expand the current understanding of bacterial diversity in the speleothems from Krem Soitan, Krem Lawbah, Krem Mawpun in Khasi Hills, Meghalaya, India. High microbial enumerations were observed on dilute nutrient agar (5.3 × 103 to 8.8 × 105) followed by M9 minimal medium (4 × 104 to 1.7 × 105) and R2A medium (1.0 × 104 to 5.7 × 105). A total of 826 bacterial isolates were selected and preserved for the study. 295 bacterial isolates were identified using MALDI-TOF spectrometry and the isolates which showed no reliable peaks were further identified by 16S rRNA gene sequencing. 91% of the total bacterial diversity was dominated by Proteobacteria and Actinobacteria. The other important phyla detected include the Firmicutes (7.45%), Deinococcus-Thermus (0.33%) and Bacteroidetes (0.67%). At the genus level, Pseudomonas (55%) and Arthrobacter (23%) were ubiquitous followed by Acinetobacter, Bacillus, Brevundimonas, Deinococcus, Flavobacterium, Paenibacillus, Pseudarthrobacter. Multivariate statistical analysis indicated that the bacterial genera formed separate clusters depending on the geochemical constituents in the spring waters suitable for their growth and metabolism. A culture-dependent approach was employed for elucidating the community structure colonizing the speleothems and wall deposits in the caves using MALDI-TOF and 16S rRNA gene sequencing. To the best of our knowledge, there are no previous geomicrobiological investigations in these caves and this study is a pioneering culture dependent study of the microbial community with many cultured isolates.
Separation of Emulsified Oil from Industrial Wastewater Using Poly Silicate Ferro-Aluminum Sulphate (PSFAS): A Novel Methodology for the Attainment of Very High Separation Efficiency
Authors: Bairagi Anup Kumar , Ravikumar Kollati Prudhvi , Sahoo Abanti , Mohapatra Soumya Sanjeeb , Agrawal Sangam 
Published: December 2021

Abstract
The large amount of wastewater generated from textile industries, petroleum industries, chemical industries contains heavy metals, suspended solids, hazardous waste, oils, fatty acids, dyes, pigment etc. It is very important to improve the quality of contaminated water before it discharges into the water sources or use. In the current work, an efficient methodology has been developed to separate emulsified oil from wastewater. The emulsified oil is tried to separate by using poly silicate Ferro aluminium sulphates, a flocculent. In addition to the above, the maximum separation efficiency for the devolved process is also revealed. Using PSFA, up to 93.5 % separation efficiency is achieved, and the discussed methodology can separate emulsified forms of the oil without altering the efficiency. The dissolved solid and metal content are also considered as the controlling parameters for the separation efficiency. The optimum TDS and the metal content must be maintained at 560 mg/L and 2 mg/L, respectively, to attain maximum separation efficiency.
Evaluation of the Treatment of High Intensity Focused Ultrasound Combined With Suction Curettage for Exogenous Cesarean Scar Pregnancy
Authors: Mu Lin , Weng Huifang , Wang Xiaoyun 
Published: December 2021

Abstract
Purpose: To evaluate the effectiveness of high intensity focused ultrasound (HIFU) combined with suction curettage in the treatment of exogenous cesarean scar pregnancy (CSP). Methods: A total of 41 patients diagnosed with exogenous CSP were enrolled in this study. All patients received HIFU treatment combined with suction curettage. Results: Twenty-nine patients were administered one session of HIFU ablation. In addition, the other 12 patients received 2 HIFU sessions. Suction curettage was performed in all patients after HIFU, and no patient was converted to laparoscopy or hysterectomy. The mean blood loss during suction curettage was 99 ml. Three patients received two sessions of suction curettage. The success rate of our study was 92.68%. The mean time for serum β-HCG normalization was 23.18±3.13 days. The average menstruation recovery time was 29.38±3.34 days. Based on the blood loss during suction curettage, 41 patients were divided into a bleeding group and a control group. The size of the gestational sac in the bleeding group (3.80±0.87 cm) was larger than that in the control group (3.39±0.77 cm) (P <0.05). The thickness of the myometrium between the bladder and gestational sac in the bleeding group (2.37±0.89 mm) was less than that in the control group (2.75±0.75 mm) (P <0.05). Conclusion: The results suggested that HIFU combined with suction curettage could be considered an effective treatment for exogenous CSP of < 9 weeks. The size of the gestational sac and the thickness of the myometrium between the bladder and gestational sac might be high-risk factors for blood loss during this treatment.