bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of a HPLC-Q/TOF Method with Post-column Compensation
for Separation and Identification of Polar Impurities in Cytidine Disodium
Triphosphate for Injection
Authors: Gu Xiao , Huang Qiaoqiao , Zheng Jinqi , Zhao Mingjuan , Yang Lishi , Chen Yue 
Published: December 2021

Background:
Cytidine Disodium Triphosphate (CTP-2Na) for injection is mainly used
for treating nervous system diseases. Currently, there are few studies focused on the separation
and identification of polar impurities in CTP-2Na for injection, which is important for ensuring
drug safety and efficacy.

Objective:
The study aimed to establish an HPLC-Q/TOF method for the separation and identification
of polar impurities in CTP-2Na for injection.

Methods:
Chromatographic separation was achieved on a Waters Atlantis T3 column using 5 mM
aqueous ammonium acetate solution as the mobile phase in an isocratic elution mode. A postcolumn
compensation technology was used to improve the ionization efficiency of impurities in
the spray chamber.

Results:
Three polar impurities (disodium cytidine tetraphosphate, disodium cytidine diphosphate,
disodium cytidine monophosphate) were detected in CTP-2Na for injection. The former one is
probably the overreaction product during the production of CTP-2Na, the latter two were reported
as degradation products. The fragmentation patterns of cytidine phosphate compounds in negative
ion mode are summarized.

Conclusion:
This study provides a good reference for the separation and identification of polar
impurities in nucleotide drugs.
Design and Synthesis of Novel Quinazolinone-Based Oxobutanenitrile Derivatives as
Antiproliferative Agents Targeting Human Breast Cancer
Authors: Ezz-ElDien Eman Salah , AboulWafa Omaima Mohamed , Daabees Hoda Mohamed Gamal El-Din 
Published: December 2021

Background:
Breast cancer (BC) is among the leading causes of death in women worldwide. Medical interest
has focused on quinazolinone derivatives approved and utilized in antitumor medications.

Objective:
Novel quinazolinone-based oxobutanenitrile derivatives were designed, synthesized, and screened for in
vitro anti-breast cancer activity.

Methods:
The antiproliferative activities were determined using MTT assay against MCF-7 and MDA-MB-231 cell
lines. EGFR, ARO, and caspase-9 enzymes were selected to explore the mechanism of action of the most potent compounds.

Results:
Tested compounds showed better EGFRIs than ARIs. In addition, significant overexpression of caspase-9
level in treated MCF-7 breast cell line samples was observed with the most active compounds. The thienyl derivative 5
induced the greatest activation in caspase-9 level in treated MCF-7 breast cancer samples. The o-tolylhydrazone 3b,
exhibiting promising ARO inhibition and weak EGFR inhibition, produced a noticeable high overexpression of caspase-
9 and showed pre-G1 apoptosis and cell cycle arrest at G2/M phase for MCF-7 cells and at S-phase for MDA-MB-
231 cells. Docking results revealed that 3b elicited binding affinities to ARO comparable to those of letrozole.

Conclusion:
The obtained results support the therapeutic importance of some of these compounds as anti-breast cancer
agents in light of the simple methodology used for their synthesis. Their design offered a way for the optimization and
development of apoptotic quinazolinone-based ARO and EGFR inhibitors.
Erythrocyte Indices and Long-Term Blood Pressure Variability in Military
Males
Authors: Lai Shiue-Wei , Tsai Kun-Zhe , Wang Shen-Huei , Lin Yu-Kai , Lin Yen-Po , Lin Gen-Min 
Published: December 2021

Backgrounds:
Severe microcytic anemia has been associated with BP changes.

Aims and Objectives:
Whether the erythrocyte indices are associated with long-term BPV is unknown.
This study aimed to investigate the association of hemoglobin levels and erythrocyte size
with long-term blood pressure variability (BPV) in young males.

Methods:
This study included 1,112 healthy military males, averaging 32 years of age, in Taiwan.
All participants took a measurement of systolic and diastolic BP (SBP and DBP) every two-year
from 2012 to 2018 (2012-14, 2014-15, 2015-16, 2016-18). Levels of hemoglobin and mean corpuscular
volume (MCV) of erythrocytes were obtained at the first visit. Long-term BPV was assessed
by the standard deviation (SD) and average real variability (ARV). Multivariate linear regression
analysis with adjustment for the baseline BP levels and other covariates was used to elucidate the
association.

Results:
Hemoglobin levels were borderline positively correlated with SD DBP (β and standard errors
= 0.016 (0.009), P =0.06). In those with hemoglobin levels of 10.0-13.9 g/dL, hemoglobin was
negatively correlated with SDSBP (β= -0.039 (0.018), P =0.03). In contrast, MCV levels were borderline
positively correlated with SDSBP (β =0.085 (0.052), P =0.09). In those with MCV levels <80
fL, MCV was positively correlated with SDSBP and ARVSBP (β= 0.445 (0.210) and 0.286 (0.149), p
= 0.03 and 0.05, respectively).

Conclusion:
There were inconsistent patterns for the associations of erythrocyte indices with longterm
BPV. We found a U-shaped relationship of hemoglobin levels with systolic BPV, whereas
there was a positive linear relationship of hemoglobin and MCV levels with diastolic BPV, respectively.
Challenges Faced by Nurses in the Provision of Health Care to Men in Primary Care, Brazil
Authors: de Sousa Anderson Reis , Leal Borges Cléa Conceição , Araújo Isabella Félix Meira , Almeida Éric Santos , de Andrade Nhamuave Edson , Escarcina Jesús Enrique Patiño , Escobar Oscar Javier Vergara , Pereira Álvaro 
Published: December 2021

Introduction:
The provision of primary care to men by nurses has been relevant, but there are challenges to be overcome.

Objective:
To analyze the challenges faced by Brazilian nurses in the provision of primary care to men.

Methods:
This was a qualitative descriptive study carried out by including 40 nurses working in the Family Health Strategy of a Basic Health Unit in Northeastern Brazil. Individual in-depth interviews were carried out and analyzed using the Discourse of the Collective Subject (CSD) method, supported by the Praxis Intervention Theory for Nursing in Collective Health – Tipesc.

Results:
The challenges faced by nurses in the provision of care to men originate in the academy and are transposed into professional practice, manifesting as limitations in the work process, lack of a specific health agenda, difficulties in raising male adherence, and professional demotivation.

Conclusion:
These challenges significantly affect the development and promotion of men’s health, resulting in the maintenance of the indicators of male morbidity and mortality in Brazil.
Meanings Assigned by Primary Care Professionals to Male Prenatal Care: A Qualitative Study
Authors: Almeida Sousa Bruna Sabrina , Almeida Camila Aparecida Pinheiro Landim , dos Santos Joseane Rodrigues , Lago Eliana Campêlo , de Oliveira Jéssika Felix , Areas da Cruz Tatiana , Lima Shirley Verônica Melo Almeida , Camargo Emerson Lucas Silva 
Published: December 2021

Background:Considering recent strategies used in prenatal care, the involvement of fathers has been considered an important factor in ensuring that pregnancy and delivery are successful.Objective:The aim of this study is to identify the meanings assigned by primary health care professionals to male prenatal care.Methods:This is a descriptive study with a qualitative approach. A total of 19 interviews were conducted with primary health care professionals registered in the City Health Department of Teresina, Piauí, Brazil. Data were collected through semi-structured interviews and analyzed according to the Collective Subject Discourse methodology.Results:Three themes emerged from the reports’ analysis: The importance of the role of fathers in the gestational process, attitudes of men toward male prenatal care and formal education and training in primary health care. Health practitioners understand the importance of male prenatal care but reported they lack proper training to provide effective care.Conclusion:The expansion of continuing education strategies focusing on male prenatal care and directed to primary health care professionals is recommended to promote greater adherence on the part of fathers in prenatal care, with the purpose of strengthening bonds and improving the care provided to the entire family. The humanized care can facilitate the approach of the paternal figure during male prenatal care.
The Effect of Dipeptidyl Nitrile Derivatives on Pancreatic Ductal Adenocarcinoma
Cells In Vitro
Authors: Botelho Sabrina Mendes , dos Reis Rocho Fernanda , Cianni Lorenzo , Montanari Carlos A. , Leitão Andrei 
Published: December 2021

Aims:
This study aims to evaluate the bioactivity of dipeptidyl nitrile inhibitors of human
cysteine cathepsins that could work as anticancer agents in a drug discovery and development
project.

Background:
Human lysosomal cysteine proteases promote cancer progression, migration, and metastasis,
targeted by inhibitors.

Objective:
Here, 19 cysteine protease inhibitors known as dipeptidyl nitriles were tested using MIA
PaCa-2 pancreatic cancer cells and Balb/3T3 clone A31 non-tumoral mouse fibroblasts.

Method:
In vitro assays evaluated cell migration, colony formation, inhibition of the enzymatic activity
in cell lysates, and combination therapy with gemcitabine.

Result:
There were mixed results; the inhibitors reduced the number of colonies but did not affect
the total area. Cells migrated despite enzyme inhibition by Neq0709 and Neq0712. As expected,
the compounds were non-cytotoxic; they improved the potency of gemcitabine in the combined
therapy assay, especially for Neq0707.

Conclusion:
In summary, our findings revealed the complexity of dealing with the translation from
biochemical to cell-based assays in the hit-to-lead step.
Work effectiveness in relation with communication, workload, and work discipline
Authors: Meianto Subangkit , Bahiramsyah Achmad , Endri Endri 
Published: December 2021

This study aims to determine the effect of Aeronautical Communication Officer Competence, Workload, and Work Discipline on the Work Effectiveness of Apron Movement Service Personnel at Soekarno - Hatta International Airport Cengkareng. Data collection techniques were carried out by distributing questionnaires using a Likert measurement scale. This research was conducted on 68 respondents using a quantitative descriptive approach and hypothesis testing. The collected data was then analyzed using Structural Equation Modeling (SEM) analysis techniques through SmartPLS (Partial Least Square) software. The results of this study indicate that the Competence of Aeronautical Communication Officer, Workload and Work Discipline of Apron Movement Service Personnel have a positive and significant impact on the Work Effectiveness of Apron Movement Service Personnel at Soekarno - Hatta International Airport.
Student, Staff, and Faculty Perspectives on Intimate Partner and Sexual Violence on 3 Public University Campuses: Protocol for the UC Speaks Up Study and Preliminary Results
Authors: Wagman Jennifer A , Amabile Claire , Sumstine Stephanie , Park Eunhee , Boyce Sabrina , Silverman Jay , Fielding-Miller Rebecca , Oaks Laury , Swendeman Dallas 
Published: December 2021

Background
Intimate partner and sexual violence are pervasive public health issues on college and university campuses in the United States. Research is recommended for creating and maintaining effective, relevant, and acceptable prevention programs and response services for student survivors.

Objective
The University of California (UC) Speaks Up study aims to examine factors contributing to intimate partner and sexual violence on 3 UC campuses and use the findings to develop and test interventions and policies to prevent violence, promote health, and lay the groundwork for subsequent large-scale quantitative research.

Methods
A mixed methods study was conducted at UC Los Angeles, UC San Diego, and UC Santa Barbara. Phase I (2017-2020) involved a resource audit; cultural consensus modeling of students’ perceptions of sexual consent; in-depth interviews (IDIs) and focus group discussions with students to understand perceptions of campus environment related to experiences as well as prevention of and responses to violence; and IDIs with faculty, staff, and community stakeholders to investigate institutional and community arrangements influencing students’ lives and experiences. Phase II (2020-ongoing) involves IDIs with student survivors to assess the use and perceptions of campus and community services. Qualitative content analysis is used to generate substantive codes and subthemes that emerge, using a thematic analysis approach.

Results
In January 2019, we conducted 149 free-listing interviews and 214 web-based surveys with undergraduate and graduate and professional students for the cultural consensus modeling. Between February 2019 and June 2019, 179 IDIs were conducted with 86 (48%) undergraduate students, 21 (11.7%) graduate and professional students, 34 (19%) staff members, 27 (15.1%) faculty members, and 11 (6.1%) community stakeholders, and 35 focus group discussions (27/35, 77% with undergraduate students and 8/35, 23% with graduate and professional students) were conducted with 201 participants. Since September 2020, 50% (15/30) of the planned student survivor interviews have been conducted. This segment of data collection was disrupted by the COVID-19 pandemic. Recruitment is ongoing.

Conclusions
Data analysis and phase II data collection are ongoing. The findings will be used to develop and test interventions for preventing violence, promoting health and well-being, and ensuring that survivor services are relevant and acceptable to and meet the needs of all individuals in the campus community, including those who are typically understudied. The findings will also be used to prepare for rigorous, UC–system-wide public health prevention research.

International Registered Report Identifier (IRRID)
DERR1-10.2196/31189
The effects of Pyrantel-Oxantel on the Dipylidium caninum tapeworm: An in vitro study
Authors: Millán-Orozco Jair , Millán-Orozco Jersson , Betancourt-Alonso Miguel Ángel , Barrera-Molina América Ivette , Valledor María Soledad , Méndez Virginia , Larrea Alejandra , Lima Martín Sebastián , Morán-Martínez Javier , Betancourt-Martínez Nadia Denys , Aguilar-Marcelino Liliana 
Published: December 2021

The present study aimed to evaluate, in vitro, the cestocidal effect of Pyrantel-Oxantel on the Dipylidium caninum tapeworm. Each intestine sample was obtained by means of a transversal incision of the abdominal area of each euthanized canine subject, individually dissected via longitudinal incision, and examined for the presence of D. caninum. An optical microscope was used to identify and verify proglottid morphology and viability based on its macroscopic appearance. The cestocidal effects of Pyrantel-Oxantel (75 mg pyrantel pamoate; 75 mg oxantel pamoate) were assessed in adult tapeworms (treated group, n= 21; control group, n= 21) placed on Petri dishes and incubated at 37 °C. One-hour post-incubation, the D. caninum cestodes treated with Pyrantel-Oxantel presented a 28 % decrease (P=0.001) in motility, which rose to a 52 % (P=0.0001) decrease by the end of the second hour. The control group (P=0.0001) presented 55.7 % motility for at least the first six hours of incubation and 4.2 % (P=0.001) motility by the end of the study, while 0 % motility was observed in the treated group by the end of the study. Pyrantel-Oxantel had a lethal effect (P=0.0001) on adult D. caninum, with 100 % mortality observed 6 h after in vitro post-incubation, while the control group presented 55.7 % viability after the same time period. In addition, Pyrantel-Oxantel reduced (P=0.001) tegument thickness by 42.5 % (10.24 ± 0.21 µm), while this was 17.81 ± 0.33 µm for the control group. The results of this study indicate that Pyrantel-Oxantel has a therapeutic effect on the presence of D. caninum, inducing both a reduction of the tegument thickness and increased mortality.
Effects of Local Anesthetics With Vasoconstrictors on Dental Pulp Blood Flow and Oxygen Tension
Authors: Tachibana Keikoku , Kasahara Masataka , Matsuura Nobuyuki , Ichinohe Tatsuya 
Published: December 2021

Objective:
The aim of this study was to investigate the changes in pulpal blood flow (PBF) and pulpal oxygen tension (PpulpO2) after injecting local anesthetics with vasoconstrictors.

Methods:
Under general anesthesia, male Japanese White rabbits were injected with 0.6 mL of 2% lidocaine with 1:80,000 epinephrine (LE) or 3% propitocaine (prilocaine) with 0.03 IU felypressin (PF) at the apical area of the lower incisor.

Results:
Relative to baseline, PBF and PpulpO2 significantly decreased 5 minutes after LE or PF injection as compared with saline. The decrease in PBF was significantly lower in the LE group than in the PF group. Although the LE group had a larger decrease in PpulpO2 relative to baseline than the PF group did, that difference was not significant. PBF and PpulpO2 recovered to baseline faster in the PF group than in the LE group.

Conclusion:
The injection of local anesthetic solutions containing vasoconstrictors (LE or PF) transiently caused significant decreases in PBF that resulted in significant decreases in PpulpO2. The recovery of PpulpO2 was faster than PBF regardless of the vasoconstrictor used.
Study of microbiocenosis of fish gills and intestines in their cultivation using preparations based on bacteriophages
Authors: Zhandalgarova Adelya Dzumaniyashevna , Bakhareva Anna Aleksandrovna , Grozesku Yulia Nikolaevna , Nevalennyy Aleksandr 
Published: December 2021

Nowadays bacteriophages are widely used in veterinary medicine, bio-technology, agriculture and in particular in aquaculture. Narrow specificity affecting the bacterial microflora and selective influence on the pathogenic and opportunistic microorganisms allow using the bacteriophages for diagnosing, preventing and treating the diseases of farm animals and fish. The emergence of antibiotic-resistant strains of microorganisms involves the search for new drugs to combat them. In this regard, phage therapy is an alternative to antimicrobial treatment of bacterial diseases of animals and fish. Despite the lack of data on the bacteriophage-based drugs applications in aquaculture, this direction is found to be promising and relevant. There is given a comparative analysis of the microbiocenosis of the gills and intestines of two-year-olds of red tilapia farmed in the water with adding the poly-valence pyobacteriophage and fed by compound feed treated by the preparation. It has been found that the dominant position in the intestinal microbiocenosis of red tilapia belongs to Micrococcus bacteria, whose share made 45.7% of all isolates of the gastrointestinal tract.
By the frequency of occurrence, the second place is taken by the Bacterium species (33.2%), the third place - Staphylococcus (16.1%) species. The remaining groups of intestinal microorganisms are presented by a significantly number of isolates. It has been stated that the gills microflora was represented by a smaller species composition compared to the digestive tract microbiota. Isolates of the Bacterium and Staphylococcus bacteria were dominating, while the Actinomycetales and Bacillus microorganisms were represented in smaller numbers (1-2 colonies). The addition of a bacteriophage to water and its processing of compound feed helps to reduce the contamination of gills and reduce pathogenic microflora in the intestines of fish.
Complications, prognostic factors, and long-term outcomes for dogs with brachycephalic obstructive airway syndrome that underwent H-pharyngoplasty and ala-vestibuloplasty: 423 cases (2011–2017)
Authors: Carabalona Julien P.R. , Le Boedec Kevin , Poncet Cyrill M. 
Published: December 2021

Abstract

OBJECTIVE
To describe the H-pharyngoplasty procedure, report the outcomes of dogs with brachycephalic obstructive airway syndrome (BOAS) treated with ala-vestibuloplasty and H-pharyngoplasty with a CO2 laser, and identify prognostic factors.

ANIMALS
423 dogs.

PROCEDURES
Medical records of dogs admitted for BOAS from 2011 to 2017 were reviewed. Dogs were included if they were treated with ala-vestibuloplasty and H-pharyngoplasty with a CO2 laser. Signalment, physical examination findings, grades at admission of clinical signs associated with respiratory and digestive systems, diagnostic test results, postoperative treatments, and short-term follow-up results were extracted from medical records. Long-term follow-up of > 12 months was evaluated via questionnaire. Generalized ordered logistic regression was used for bivariable and multivariable analyses.

RESULTS
Overall mortality rate was 2.6%. Median duration of follow-up was 36 months (12 to 91 months), and 341 (80.6%) dog owners completed the questionnaire. Major complications included respiratory distress (2.1%), heatstroke (0.5%), and bronchopneumonia (0.5%). No dogs required revision surgery. Improvement in signs associated with the respiratory and digestive systems was reported in 72% and 34% of the dogs, respectively, and owners’ satisfaction was high (97.1%). Risk of death increased by 29.8% (95% CI, 11.8% to 50.7%) for every 1-year increase in age.

CONCLUSION AND CLINICAL RELEVANCE
H-pharyngoplasty was possible in all dogs with BOAS, including those previously treated with conventional surgery and was associated with low morbidity and improved respiratory and digestive signs. H-pharyngoplasty combined with ala-vestibuloplasty may be an alternative treatment for even the most severely affected dogs.
Minimal complications observed with a modified surgical approach for treatment of canine anal sac neoplasia
Authors: Chen Carolyn L. , Lapsley Janis M. , Selmic Laura E. 
Published: December 2021

Abstract

OBJECTIVE
To describe a modified approach to closed anal sacculectomy and report the short-term outcomes of dogs that underwent the procedure for treatment of neoplasia.

ANIMALS
16 client-owned dogs.

PROCEDURES
Medical records of 1 referral hospital were reviewed to identify dogs that underwent modified closed anal sacculectomy for treatment of anal sac neoplasia between January 2018 and September 2020. Data collected included signalment, examination and diagnostic imaging findings, surgical details, intraoperative and postoperative complications, cytologic and histologic findings, adjuvant treatments, duration of follow-up, and short-term outcome.

RESULTS
15 dogs had apocrine gland anal sac adenocarcinoma, and 1 had a collision tumor. No dogs had intraoperative complications; 1 dog had a minor postoperative complication (paraparesis) that resolved. The median duration of postoperative follow-up was 286 days (range, 67 to 777 days). One dog had confirmed local disease recurrence 290 days after surgery, and 1 had suspected local disease recurrence 203 days after surgery and was euthanized because of systemic disease progression.

CONCLUSIONS AND CLINICAL RELEVANCE
The modified closed anal sacculectomy was well tolerated in this sample of dogs, with minimal short-term complications. This study provided evidence to justify evaluation of the procedure in a larger number of dogs and assessment of the effects of procedural modifications on postoperative complication rates and time to local recurrence.