bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution of neutron capture elements in dwarf galaxies
Authors: Molero Marta , Romano Donatella , Reichert Moritz , Matteucci Francesca , Arcones Almudena , Cescutti Gabriele , Simonetti Paolo , Hansen Camilla Juul , Lanfranchi Gustavo A 
Published: May 2021

ABSTRACT
We study the evolution of europium (Eu) and barium (Ba) abundances in Local Group dwarf spheroidal and ultrafaint dwarf galaxies by means of detailed chemical evolution models and compare our results with new sets of homogeneous abundances. The adopted models include gas infall and outflow and have been previously tested. We investigate several production scenarios for r-process elements: merging neutron stars and magnetorotational-driven supernovae. Production of Ba through the main s-process acting in low- and intermediate-mass stars is considered as well. We also test different sets of nucleosynthesis yields. For merging neutron stars we adopt either a constant and short delay time for merging or a delay time distribution function. Our simulations show that (i) if r-process elements are produced only by a quick source, it is possible to reproduce the [Eu/Fe] versus [Fe/H], but those models fail in reproducing the [Ba/Fe] versus [Fe/H]. (ii) If r-process elements are produced only with longer delays the opposite happens. (iii) If both a quick source and a delayed one are adopted, such as magnetorotational-driven supernovae and merging neutron stars with a delay time distribution, the [Eu/Fe] abundance pattern is successfully reproduced, but models still fail in reproducing the [Ba/Fe]. (iv) On the other hand, the characteristic abundances of Reticulum II can be reproduced only if both the Eu and the r-process fraction of Ba are produced on short and constant time delays during a single merging event. We discuss also other possible interpretations, including an inhomogeneous mixing of gas that might characterize this galaxy.
The EOS/resolution conspiracy: convergence in proto-planetary collision simulations
Authors: Meier Thomas , Reinhardt Christian , Stadel Joachim Gerhard 
Published: May 2021

ABSTRACT
We investigate how the choice of equation of state (EOS) and resolution conspire to affect the outcomes of giant impact (GI) simulations. We focus on the simple case of equal-mass collisions of two Earth-like 0.5-M⊕ proto-planets showing that the choice of EOS has a profound impact on the outcome of such collisions as well as on the numerical convergence with resolution. In simulations where the Tillotson EOS is used, impacts generate an excess amount of vapour due to the lack of a thermodynamically consistent treatment of phase transitions and mixtures. In oblique collisions this enhances the artificial angular momentum (AM) transport from the planet to the circum-planetary disc reducing the planet’s rotation period over time. Even at a resolution of 1.3 × 106 particles, the result is not converged. In head-on collisions, the lack of a proper treatment of the solid/liquid-vapour phase transition allows the bound material to expand to very low densities, which, in turn, results in very slow numerical convergence of the critical specific impact energy for catastrophic disruption $Q_{\rm {RD}}^{*}$ with increasing resolution as reported in prior work. The simulations where ANalytic Equation Of State (ANEOS) is used for oblique impacts are already converged at a modest resolution of 105 particles, while head-on collisions converge when they evidence the post-shock formation of a dense iron-rich ring, which promotes gravitational re-accumulation of material. Once sufficient resolution is reached to resolve the liquid-vapour phase transition of iron in the ANEOS case, and this ring is resolved, the value of $Q_{\rm {RD}}^{*}$ has then converged.
Radio relics in PSZ2 G096.88+24.18: a connection with pre-existing plasma
Authors: Jones A , de Gasperin F , Cuciti V , Hoang D N , Botteon A , Brüggen M , Brunetti G , Finner K , Forman W R , Jones C , Kraft R P , Shimwell T , van Weeren R J 
Published: May 2021

ABSTRACT
Giant radio relics are arc-like structures of diffuse, non-thermal synchrotron radiation that trace shock waves induced by galaxy cluster mergers. The particle (re-)acceleration mechanism producing such radio relics is unclear. One major open question is whether relics can be formed directly from a population of thermal seed electrons, or if pre-existing relativistic seed electrons are required. In some cases, active galactic nuclei (AGNs) can provide such a population of sub-GeV electrons. However, it is unclear how common this connection is. In this paper, we present LOFAR 140 MHz and Very Large Array L-band radio observations, as well as Chandra data of PSZ2 G096.88+24.18, a merging galaxy cluster system hosting a pair of radio relics. A large patch of diffuse emission connects a bright radio galaxy with one of the relics, likely affecting the properties of the relic. We find that the most plausible explanation for the connection is that the merger shock wave has passed over an AGN lobe. The shock passing over this seed population of electrons has led to an increased brightness in the relic only in the region filled with seed electrons.
Imaging of native transcription and transcriptional dynamicsin vivousing a tagged Argonaute protein
Authors: Toudji-Zouaz Amel , Bertrand Vincent , Barrière Antoine 
Published: May 2021

AbstractA flexible method to image unmodified transcripts and transcription in vivo would be a valuable tool to understand the regulation and dynamics of transcription. Here, we present a novel approach to follow native transcription, with fluorescence microscopy, in live C. elegans. By using the fluorescently tagged Argonaute protein NRDE-3, programmed by exposure to defined dsRNA to bind to nascent transcripts of the gene of interest, we demonstrate transcript labelling of multiple genes, at the transcription site and in the cytoplasm. This flexible approach does not require genetic manipulation, and can be easily scaled up by relying on whole-genome dsRNA libraries. We apply this method to image the transcriptional dynamics of the heat-shock inducible gene hsp-4 (a member of the hsp70 family), as well as two transcription factors: ttx-3 (a LHX2/9 orthologue) in embryos, and hlh-1 (a MyoD orthologue) in larvae, respectively involved in neuronal and muscle development.
Dissecting and tuning primer editing by proofreading polymerases
Authors: Gohl Daryl M , Auch Benjamin , Certano Amanda , LeFrançois Brice , Bouevitch Anne , Doukhanine Evgueni , Fragel Christina , Macklaim Jean , Hollister Emily , Garbe John , Beckman Kenneth B 
Published: May 2021

Abstract
Proofreading polymerases have 3′ to 5′ exonuclease activity that allows the excision and correction of mis-incorporated bases during DNA replication. In a previous study, we demonstrated that in addition to correcting substitution errors and lowering the error rate of DNA amplification, proofreading polymerases can also edit PCR primers to match template sequences. Primer editing is a feature that can be advantageous in certain experimental contexts, such as amplicon-based microbiome profiling. Here we develop a set of synthetic DNA standards to report on primer editing activity and use these standards to dissect this phenomenon. The primer editing standards allow next-generation sequencing-based enzymological measurements, reveal the extent of editing, and allow the comparison of different polymerases and cycling conditions. We demonstrate that proofreading polymerases edit PCR primers in a concentration-dependent manner, and we examine whether primer editing exhibits any sequence specificity. In addition, we use these standards to show that primer editing is tunable through the incorporation of phosphorothioate linkages. Finally, we demonstrate the ability of primer editing to robustly rescue the drop-out of taxa with 16S rRNA gene-targeting primer mismatches using mock communities and human skin microbiome samples.
A synthetic RNA-mediated evolution system in yeast
Authors: Jensen Emil D , Laloux Marcos , Lehka Beata J , Pedersen Lasse E , Jakočiūnas Tadas , Jensen Michael K , Keasling Jay D 
Published: May 2021

Abstract
Laboratory evolution is a powerful approach to search for genetic adaptations to new or improved phenotypes, yet either relies on labour-intensive human-guided iterative rounds of mutagenesis and selection, or prolonged adaptation regimes based on naturally evolving cell populations. Here we present CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) of genomic loci using evolving chimeric donor gRNAs continuously delivered from an error-prone T7 RNA polymerase, and directly introduced as RNA repair donors into genomic targets under either Cas9 or dCas9 guidance. We validate CRAIDE by evolving novel functional variants of an auxotrophic marker gene, and by conferring resistance to a toxic amino acid analogue in baker's yeast Saccharomyces cerevisiae with a mutation rate &gt;3,000-fold higher compared to spontaneous native rate, thus enabling the first demonstrations of in vivo delivery and information transfer from long evolving RNA donor templates into genomic context without the use of in vitro supplied and pre-programmed repair donors.
Gawky modulates MTF-1-mediated transcription activation and metal discrimination
Authors: Jia Ruirui , Song Zhenxing , Lin Jiamei , Li Zhengguo , Shan Ge , Huang Chuan 
Published: May 2021

Abstract
Metal-induced genes are usually transcribed at relatively low levels under normal conditions and are rapidly activated by heavy metal stress. Many of these genes respond preferentially to specific metal-stressed conditions. However, the mechanism by which the general transcription machinery discriminates metal stress from normal conditions and the regulation of MTF-1-meditated metal discrimination are poorly characterized. Using a focused RNAi screening in Drosophila Schneider 2 (S2) cells, we identified a novel activator, the Drosophila gawky, of metal-responsive genes. Depletion of gawky has almost no effect on the basal transcription of the metallothionein (MT) genes, but impairs the metal-induced transcription by inducing the dissociation of MTF-1 from the MT promoters and the deficient nuclear import of MTF-1 under metal-stressed conditions. This suggests that gawky serves as a ‘checkpoint’ for metal stress and metal-induced transcription. In fact, regular mRNAs are converted into gawky-controlled transcripts if expressed under the control of a metal-responsive promoter, suggesting that whether transcription undergoes gawky-mediated regulation is encrypted therein. Additionally, lack of gawky eliminates the DNA binding bias of MTF-1 and the transcription preference of metal-specific genes. This suggests a combinatorial control of metal discrimination by gawky, MTF-1, and MTF-1 binding sites.
Disturbed Amazonian forests support diminished breeding bird communities
Authors: Rutt Cameron L , Kaller Michael D , Stouffer Philip C 
Published: May 2021

AbstractTwenty percent of the Brazilian Amazon has now been deforested, and deforestation rates are increasing. This compels us to evaluate the conservation potential of human-modified landscapes; yet the ecological value of regenerating and fragmented Amazonian forests remains poorly understood. To date, most faunal studies in disturbed forests have examined metrics derived from presence or abundance. Although valuable, these data cannot tell us how old-growth species are using disturbed forests. In this study, we complement presence data with breeding observations to assess more comprehensively the habitat quality of disturbed forests in central Amazonia. We synthesized breeding evidence from standardized passive mist-netting, mixed-species flock-following, and opportunistic nest-searching across a full annual cycle in 30–35-year-old secondary forest, 10-ha fragments, and undisturbed forest. We then used multistate occupancy models to estimate the number of undetected breeding species in each forest type, which illustrated that fewer species bred in secondary forest (–43%) and 10-ha fragments (–17%) than in undisturbed forest. Both these losses are larger than the associated decrease in species richness alone (–17% and –10%, respectively). Notably, we confirmed breeding by only three terrestrial and near-ground insectivores in fragments and secondary forest combined (of the 9 species found in undisturbed forest). Disturbed forests also supported fewer breeding individuals (–35% to –50%) and, in secondary forest, fewer successful breeding attempts (–24%). Encouragingly, however, some forest-dependent birds are breeding and producing fledglings in disturbed forests, including representatives from almost every guild. This was especially apparent for mixed-species flocks and army-ant followers, two guilds that have historically been considered vulnerable to anthropogenic disturbance. Therefore, despite a loss of breeding habitat in disturbed forests, these data suggest that landscapes composed of regenerating forest and small fragments have conservation potential for forest bird populations.
Three-dimensional dust geometry of the LMC H i ridge region as revealed by the IRSF/SIRIUS survey
Authors: Furuta Takuya , Kaneda Hidehiro , Kokusho Takuma , Nakajima Yasushi , Fukui Yasuo , Tsuge Kisetsu 
Published: May 2021

Abstract
We present a new method to evaluate the dust extinction (AV) along the line of sight using the InfraRed Survey Facility (IRSF) near-infrared (NIR) data of the Large Magellanic Cloud (LMC) H i ridge region. In our method, we estimate an AV value for each star from the NIR color excess and sort them from bluer to redder in each line of sight. Using the percentile values of the sorted AV, we construct a new three-dimensional AV map. We compare the resultant AV map with the total hydrogen column density N(H) traced by velocity-resolved H i and CO observations. In the LMC H i ridge region, Fukui et al. (2017, PASJ, 69, L5) found two velocity components and an intermediate velocity one bridging them. Comparing our three-dimensional AV maps with N(H) maps at the different velocities, we find that the dust geometry is consistent with the scenario of ongoing gas collision between the two velocities as suggested in the previous study. In addition, we find a difference by a factor of 2 in AV/N(H) between the two velocity components, which suggests that inflow gas from the Small Magellanic Clouds (SMC) is mixed in this region. Overall, our results support triggered star formation in 30 Doradus due to the large-scale gas collision caused by tidal interaction between the LMC and the SMC.
Origin and Evolution of the Fatu Kapa Magmatic System (North-Western Lau Back-arc Basin): Insight on the Genesis of High-Silica Lavas
Authors: Jeanvoine Aurélien , Chazot Gilles , Labanieh Shasa , Pelleter Ewan , Fouquet Yves 
Published: May 2021

Abstract
The South Eastern Futuna Volcanic Zone (SEFVZ; 50 km × 80 km) is located in the NW of the Lau back-arc basin at the northern boundary of the Futuna and YAO microplates. Fatu Kapa is an area (∼15 km × 10 km) of diffuse volcanic activity at the center of the SEFVZ and at the intersection of two volcanic axes with the Futuna fracture zone. At this place, the volcanic, tectonic, and hydrothermal activities are enhanced. The most differentiated volcanic series of the whole Futuna region are found in the Fatu Kapa area. Based on major and trace element data, four groups of volcanic rocks are distinguished in the Fatu Kapa area. The main groups form a well-defined transitional suite from basalts to andesitic and dacitic lavas that display enrichments in Zr, Cl and heavy rare earth elements. The most mafic lavas of this group are extracted from an Indian-type depleted mantle mixed with a component with trace element and isotopic characteristics similar to the nearby Samoa mantle plume. There is no evidence of subduction input. Petrological modelling shows that the mafic melts of the main group (Group 1A) evolve towards trachyandesites through fractional crystallization coupled with assimilation of 10 % of a Cl-rich and Th-poor melt generated by low-degree (≤2 %) partial melting of a hydrothermally altered basaltic crust. Rocks of Groups 1B(a) and 1B(b) were then generated by mixing of a mafic melt from Group 1A with a melt obtained by partial melting of andesitic and hydrothermally altered wallrock in shallow magma chambers. Extremely high Cl concentrations can be explained by assimilation of interstitial K- and Cl-rich brines in the pore water of the hydrothermally altered volcanic rocks before melting of these rocks. This is in good agreement with unusual extension of the Fatu Kapa hydrothermal area and indicates that, in the north of the Lau basin, the microplate boundaries are privileged zones to generate a complex felsic magmatism within areas of widespread hydrothermal alteration. Our study confirms the importance of assimilation and fractional crystallization and mixing processes in generating high-silica lavas in back-arc environments decoupled from subduction.
MITOGEN-ACTIVATED PROTEIN KINASE 4 impacts leaf development, temperature, and stomatal movement in hybrid aspen
Authors: Witoń Damian , Sujkowska-Rybkowska Marzena , Dąbrowska-Bronk Joanna , Czarnocka Weronika , Bernacki Maciej , Szechyńska-Hebda Magdalena , Karpiński Stanisław 
Published: May 2021

Abstract
Stomatal movement and density influence plant water use efficiency and thus biomass production. Studies in model plants within controlled environments suggest MITOGEN-ACTIVATED PROTEIN KINASE 4 (MPK4) may be crucial for stomatal regulation. We present functional analysis of MPK4 for hybrid aspen (Populus tremula × tremuloides) grown under natural field conditions for several seasons. We provide evidence of the role of MPK4 in the genetic and environmental regulation of stomatal formation, differentiation, signaling, and function; control of the photosynthetic and thermal status of leaves; and growth and acclimation responses. The long-term acclimation manifested as variations in stomatal density and distribution. Short-term acclimation responses were derived from changes in the stomatal aperture. MPK4 localized in the cytoplasm of guard cells (GCs) was a positive regulator of abscisic acid (ABA)-dependent stomatal closure and nitric oxide metabolism in the ABA-dependent pathways, while to a lesser extent, it was involved in ABA-induced hydrogen peroxide accumulation. MPK4 also affected the stomatal aperture through deregulation of microtubule patterns and cell wall structure and composition, including via pectin methyl-esterification, and extensin levels in the GC wall. Deregulation of leaf anatomy (cell compaction) and stomatal movement, together with increased light energy absorption, resulted in altered leaf temperature, photosynthesis, cell death, and biomass accumulation in mpk4 transgenic plants. Divergence between absorbed energy and assimilated energy is a bottleneck, and MPK4 can participate in the control of energy dissipation (thermal effects). Furthermore, MPK4 can participate in balancing the photosynthetic energy distribution via its effective use in growth or redirection to acclimation/defense responses.
Police Accountability in the USA: Gaining Traction or Spinning Wheels?
Authors: Archbold Carol A 
Published: May 2021

AbstractPolice accountability has long been a topic of discussion and debate among police practitioners, politicians, and scholars. This topic re-emerges every time there is highly publicized police shooting or incident of police misconduct. This article provides an overview of the current state of police accountability in the USA. This overview includes several examples of the ways in which police accountability has been enhanced in recent years at the local, state, and federal levels. Next, several obstacles to the progression of police accountability in the USA are discussed including a lack of ‘buy-in’ by some police personnel; limited research on the efficacy of common police accountability tools; lack of financial resources; lack of support and guidance by the federal government in recent years; and police unions. Finally, the article concludes with suggestions for overcoming some of the impediments associated with police accountability in the USA.
Spontaneous slow oscillation—slow spindle features predict induced overnight memory retention
Authors: Dehnavi Fereshteh , Koo-Poeggel Ping Chai , Ghorbani Maryam , Marshall Lisa 
Published: May 2021

AbstractStudy ObjectivesSynchronization of neural activity within local networks and between brain regions is a major contributor to rhythmic field potentials such as the EEG. On the other hand, dynamic changes in microstructure and activity are reflected in the EEG, for instance slow oscillation (SO) slope can reflect synaptic strength. SO-spindle coupling is a measure for neural communication. It was previously associated with memory consolidation, but also shown to reveal strong interindividual differences. In studies, weak electric current stimulation has modulated brain rhythms and memory retention. Here, we investigate whether SO-spindle coupling and SO slope during baseline sleep are associated with (predictive of) stimulation efficacy on retention performance.MethodsTwenty-five healthy subjects participated in three experimental sessions. Sleep-associated memory consolidation was measured in two sessions, in one anodal transcranial direct current stimulation oscillating at subjects individual SO frequency (so-tDCS) was applied during nocturnal sleep. The third session was without a learning task (baseline sleep). The dependence on SO-spindle coupling and SO-slope during baseline sleep of so-tDCS efficacy on retention performance were investigated.ResultsStimulation efficacy on overnight retention of declarative memories was associated with nesting of slow spindles to SO trough in deep nonrapid eye movement baseline sleep. Steepness and direction of SO slope in baseline sleep were features indicative for stimulation efficacy.ConclusionsFindings underscore a functional relevance of activity during the SO up-to-down state transition for memory consolidation and provide support for distinct consolidation mechanisms for types of declarative memories.