bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival
Authors: Stevens Sharon R. , van der Heijden Meike E. , Ogawa Yuki , Lin Tao , Sillitoe Roy V. , Rasband Matthew N. 
Published: November 2021

Ankyrin scaffolding proteins are critical for membrane domain organization and protein stabilization in many different cell types including neurons. In the cerebellum, Ankyrin-R (AnkR) is highly enriched in Purkinje neurons, granule cells, and in the cerebellar nuclei (CN). Using male and female mice with a floxed allele forAnk1in combination withNestin-CreandPcp2-Cremice, we found that ablation of AnkR from Purkinje neurons caused ataxia, regional and progressive neurodegeneration, and altered cerebellar output. We show that AnkR interacts with the cytoskeletal protein β3 spectrin and the potassium channel Kv3.3. Loss of AnkR reduced somatic membrane levels of β3 spectrin and Kv3.3 in Purkinje neurons. Thus, AnkR links Kv3.3 channels to the β3 spectrin-based cytoskeleton. Our results may help explain why mutations in β3 spectrin and Kv3.3 both cause spinocerebellar ataxia.SIGNIFICANCE STATEMENTAnkyrin scaffolding proteins localize and stabilize ion channels in the membrane by linking them to the spectrin-based cytoskeleton. Here, we show that Ankyrin-R (AnkR) links Kv3.3 K+channels to the β3 spectrin-based cytoskeleton in Purkinje neurons. Loss of AnkR causes Purkinje neuron degeneration, altered cerebellar physiology, and ataxia, which is consistent with mutations in Kv3.3 and β3 spectrin causing spinocerebellar ataxia.
ANNEALING TEMPERATURES’ EFFECTS ON MICROSTRUCTURE AND OPTICAL PROPERTIES OF Ba0.95Sr0.05TiO3 FILMS
Authors: Iriani Yofentina , Nurosyid Fahru , Mayasari Ratna , Sandi Dianisa Khoirum 
Published: November 2021

ANNEALING TEMPERATURES’ EFFECTS ON MICROSTRUCTURE AND OPTICAL PROPERTIES OF Ba0.95Sr0.05TiO3 FILMS. Ferroelectric materials, one of which is Barium Strontium Titanate (BST), can be applied for photovoltaic. Ferroelectric films function as the P-type semiconductor in the P-N junction. BST (Ba0.95Sr0.05TiO3) films have been deposited on Pt/Si (111) and quartz substrates via the CSD method prepared by spin coater. The films were annealed at various temperatures of 800 °C, 900 °C, and 1000 °C to observe the annealing temperatures' effects on the microstructure and optical properties of the BST films. From the XRD results, the intensity of diffraction peaks gets higher along with the higher annealing temperature. It thus causes the level of crystallization and the crystal size of the Ba0.95Sr0.05TiO3 films to increase. The morphology results reveal that the grains size of the Ba0.95Sr0.05TiO3 films is getting larger with the higher annealing temperature. The optical properties examined in the Ba0.95Sr0.05TiO3 films include absorbance and bandgap energy values. Values of bandgap energy show a decrease with increasing sintering temperature. The smallest bandgap energy of the Ba0.95Sr0.05TiO3 film is achieved at 1000 °C of 3.20 eV. BST films were annealed at temperature 1000 °C attained from this study can be considered as candidate for a photovoltaic ferroelectric material.
Correspondence between Site Amplification and Topographical, Geological Parameters: Collation of Data from Swiss and Japanese Stations, and Neural Networks-Based Prediction of Local Response
Authors: Bergamo Paolo , Hammer Conny , Fäh Donat 
Published: November 2021

ABSTRACTWe address the relation between seismic local amplification and topographical and geological indicators describing the site morphology. We focus on parameters that can be derived from layers of diffuse information (e.g., digital elevation models, geological maps) and do not require in situ surveys; we term these parameters as “indirect” proxies, as opposed to “direct” indicators (e.g., f0, VS30) derived from field measurements. We first compiled an extensive database of indirect parameters covering 142 and 637 instrumented sites in Switzerland and Japan, respectively; we collected topographical indicators at various spatial extents and focused on shared features in the geological descriptions of the two countries. We paired this proxy database with a companion dataset of site amplification factors at 10 frequencies within 0.5–20 Hz, empirically measured at the same Swiss and Japanese stations. We then assessed the robustness of the correlation between individual site-condition indicators and local response by means of statistical analyses; we also compared the proxy-site amplification relations at Swiss versus Japanese sites. Finally, we tested the prediction of site amplification by feeding ensembles of indirect parameters to a neural network (NN) structure. The main results are: (1) indirect indicators show higher correlation with site amplification in the low-frequency range (0.5–3.33 Hz); (2) topographical parameters primarily relate to local response not because of topographical amplification effects but because topographical features correspond to the properties of the subsurface, hence to stratigraphic amplification; (3) large-scale topographical indicators relate to low-frequency response, smaller-scale to higher-frequency response; (4) site amplification versus indirect proxy relations show a more marked regional variability when compared with direct indicators; and (5) the NN-based prediction of site response is the best achieved in the 1.67–5 Hz band, with both geological and topographical proxies provided as input; topographical indicators alone perform better than geological parameters.
РАЗРАБОТКА И ИССЛЕДОВАНИЕ НОВОГО СПОСОБА ГАЗООЧИСТКИ ОТ ЧАСТИЦ РАЗМЕРОМ МЕНЕЕ 2, 5 МКМ
Authors: Khmelev Vladimir N. , Shalunov Andrey V. , Bochenkov Alexander S. , Nesterov Viktor A. 
Published: November 2021

Ссылка для цитирования: Разработка и исследование нового способа газоочистки от частиц размером менее 2, 5 мкм / В.Н. Хмелев, А.В. Шалунов, А.С. Боченков, В.А. Нестеров // Известия Томского политехнического университета. Инжиниринг георесурсов. – 2021. – Т. 332. – № 10. – С. 127–139.
Актуальность исследования обусловлена отсутствием эффективных методов и средств борьбы с загрязнением атмосферы субмикронными частицами размером менее 2,5 мкм. Применяемое для этих целей ультразвуковое воздействие не позволяет обеспечить степень укрупнения дисперсных частиц, достаточную для их осаждения или улавливания существующим газоочистным оборудованием. Как показывают экспериментальные исследования, даже при максимальном уровне звукового давления (при превышении которого инициируется обратный процесс – диспергирование капель или разрушении агломератов частиц) эффективность ультразвуковой коагуляции оказывается недостаточной, особенно при малой счетной концентрации частиц. Это обуславливает необходимость выявление новых физических эффектов и разработки новых способов воздействия, повышающих эффективность коагуляции тонкодисперсных частиц. Цель: разработка способа для повышения эффективности ультразвуковой коагуляции частиц размером менее 2,5 мкм за счет формирования вихревых акустических течений в тонком воздушном промежутке между излучателем и отражателем и создание устройства для его практической реализации. Результаты. Предложен новый способ и устройство для очистки газов от дисперсных частиц размером менее 2,5 мкм за счет увеличения времени ультразвукового воздействия на каждую частичку и создания зон локального увеличения концентрации субмикронных частиц. Это обеспечивается за счет формирования вихревых потоков между излучающей (в виде изгибно-колеблющегося диска) и отражающей поверхностями. Локальная зона повышенной концентрации частиц формируется в периферийной области вихря за счет дрейфа частиц под действием центробежных сил из центральной области вихря к его периферии. Предложенный способ обеспечивает повышение эффективности с 13 до 50 %, в зависимости от исходной концентрации дисперсных частиц.
The role of the possibilities of the influence of traditional and modern art on the self-presentation of future teachers
Authors: Nebessayeva Zhanar , Akmara Smanova , Kuralay Bekbolatova , Gadilbek Alzhanov , Dzhaybulovna Jartybayeva Zhamila , Erkinkyzy Rsmakhanbetova Shakhrizada , Bektemirovna Kunzhigitova Gulnur 
Published: November 2021

Current research has considered the possibilities of the influence of traditional and contemporary art on self-presentation among future teachers at the stage of university professional training in the framework of the educational process and students' activities. The purpose of the article is to determine the effectiveness of the implementation of the complex influence of traditional and contemporary art in pedagogical conditions, as a way of organizing and managing the process of forming a culture of self-presentation among future teachers. The research collected data through a questionnaire and the sample consisted of 216 students. The collected data was analysed using statistical methods such as student t-tests and percentages. The main results are that the developed and implemented author's complex of possibilities for the influence of traditional and contemporary art in pedagogical conditions ensures its successful formation in future teachers at the stage of professional training at the university.

Keywords: contemporary art; professional training; self-presentation; traditional art.
Financial Performance of Telecommunication Sector Companies Before And During The Covid-19 Pandemic
Authors: Jauzaa Alifa , Hirawati Heni 
Published: November 2021

This research aims to analyze the financial performance of telecommunications sektor companies judging from profitability ratios which unclude ROA, ROE, and NPM period before and during the Covid-19 pandemi. Covid-19 has an impact on the weakening of various sektors of the economy. Nevertheless, there are still some sektors that are able togrow. One of them is the telecommunications sektor. The internet plays a big role in community activities that are generally carried out online, causing an increase in the performance of telecommuniciation issuers. The data used in this study is the financial statements of telecommunication companies listed on the Indonesia Stock Exchange quarter 2 of 2019-quarter 1 of 2021 and analyzed using descriptive methods. The results showed that the financial performance of telecommunications sektor companies was guided by the average value of profitability ratios that include ROA, ROE, and NPM obtained results that during the covid-19 pandemi were first confirmed, overall, the average financial performance of telecommunications sektor companies has increased. Assesment with the analysis of Hotteling’s T2 test also provided results that there was no difference in financial performance in terms of the profitability ratio between before and during the covid-19 pandemi
A multicenter, randomized, double-blind, placebo-controlled phase ii trial of intravenous inflammasome inhibitor (NuSepin) for the treatment of COVID-19 patients
Authors: Seong Seung-Yong , Lee Seunghwa 
Published: November 2021

Abstract
Purpose

Here we evaluated whether a novel inflammasome inhibitor targeting GPCR19 (NuSepin) provides greater benefit than placebo in patients with COVID-19 pneumonia.
Methods

We conducted a double-blind, randomized, placebo-controlled phase ii trial of intravenous NuSepin, which is an NLRP3 inflammasome inhibitor targeting GPCR19, in adults who were hospitalized with COVID-19 pneumoniae in Romania from September 2020 to March 2021. The primary outcome was the time to clinical improvement, defined as a decline of two ordinal scales (OS) from randomization on a six-category OS that ranges from 1 (discharged with normal activity) to 6 (death) (=TTCI_P). In addition, clinical improvement was also assessed by aggregated National Early Warning Score 2 (NEWS2), and TTCI_S was defined by time to NEWS2=0 from randomization, which is maintained for 24 h.
Results

TTCI_P was different between groups numerically, but not statistically. The median difference in TTCI_S was 3.5 d between the NuSepin group and the placebo group (p = 0.016) in moderate-to-severe patients (with baseline NEWS2 ≥ 5) of the PP set [recovery rate ratio = 2.7, p = 0.02], which favored improved recovery in the NuSepin group. The overall recovery rate ratio was 3.4, which favors the NuSepin group in terms of recovery rate (p = 0.0026) when the effects of covariates (use of anti-viral drugs and baseline NEWS2 ≥ 5) were adjusted. Serious adverse events were reported in a patient who received 0.2 mg/kg NuSepin (4.5%), but this was found to be unrelated to NuSepin treatment.
Conclusion

Considering that NuSepin has a favorable and tolerable safety profile, clinical improvement of hospitalized moderate-to-severe patients might be achieved with 0.2 mg/kg NuSepin significantly faster than placebo.
Cardiac and Other Presentation and Clinical Outcomes of COVID-19 Pandemic among Different Ethnic and Religious Populations in The City of Jerusalem
Authors: Asher Elad , Samuel Tal , Yagel Oren , Wolak Arik , Farkash Rivka , Durst Ronen , Ben-Chetrit Eli , Helviz Yigal , Tvito Ariella , Nir-Paz Ran , Amir Offer , Glikson Michael 
Published: November 2021

Abstract
Background: The COVID‑19 pandemic is an ongoing global pandemic. Jerusalem with its 919,400 inhabitants has a wide variety of populations, of which 62% are Jews (36% ultra-orthodox; 64% non-ultraorthodox) and 38% Arabs which were largely affected by the pandemic. The aim of our study was to understand the different presentations, course and clinical outcomes in these different ethnical and cultural groups in Jerusalem in the COVID-19 pandemic. Methods: We performed a cohort study of all COVID-19 patients admitted between March 9 - July 16, 2020 to the two university medical centers in Jerusalem. Patients were divided according to their religion and ethnicity into 3 main groups: 1) Ultra-Orthodox Jews; 2) other (non-Ultra-Orthodox) Jews and 3) Arabs. Results: Six hundred and two patients comprised the study population. Of them the 361 (60%) were Ultra-Orthodox Jews; 166 (27.5%) non-Ultra-Orthodox Jews and 75 (12.5%) Arabs. The Arab patients were younger than the Ultra-Orthodox Jews and the non-Ultra-Orthodox Jews (51±18 year-old vs. 57±21 and 59±19, respectively, p<0.01), but suffered from significantly more co-morbidities. Moreover, hemodynamic shock, ischemic ECG changes and pathological chest x-ray were all more frequent in the Ultra-Orthodox patients as compared the other groups of patients. Being an Ultra-Orthodox was independently associated with significantly higher rate of Major Adverse Cardiovascular Events (MACE) [OR=1.96; 95% CI (1.03-3.71), p<0.05]. Age was the only independent risk factor associated with increased mortality rate [OR=1.10; 95% CI (1.07 - 1.13), p<0.001]. Conclusions: The COVID-19 first phase in Jerusalem, affected different ethnical and cultural groups differently, with the Ultra-Orthodox Jews mostly affected by admission rates, presenting symptoms clinical course and MACE (Acute coronary syndrome, shock, cerebrovascular event or venous thromboembolism). It is conceivable that vulnerable populations need special attention and health planning in time of pandemic, to prevent rapid distribution and severe morbidity.
Performance of a Novel Risk Model for Deep Sternal Wound Infection after Coronary Artery Bypass Grafting
Authors: Orlandi Bianca Maria Maglia , Mejia Omar Asdrubal Vilca , Sorio Jennifer Loría , Silva Pedro Barros e , Oliveira Marco Antonio Praça , Nakazone Marcelo Arruda , Tiveron Marcos Gradim , Campagnucci Valquíria Pelliser , Lisboa Luiz Augusto Ferreira , Zubelli Jorge , Normand Sharon-Lise , Jatene Fabio Biscegli 
Published: November 2021

Abstract
Clinical prediction models for deep sternal wound infections (DSWI) after coronary artery bypass graft (CABG) surgery exist, although they have a poor impact in external validation studies. We developed and validated a new predictive model for 30-day DSWI after CABG (REPINF) and compared it with the Society of Thoracic Surgeons model (STS). The REPINF model was created through a multicenter cohort of adults undergoing CABG surgery (REPLICCAR II Study) database, using least absolute shrinkage and selection operator (LASSO) logistic regression, internally and externally validated comparing discrimination, calibration in-the-large (CL), net reclassification improvement (NRI) and integrated discrimination improvement (IDI), trained between the new model and the STS PredDeep, a validated model for DSWI after cardiac surgery. In the validation data, c-index = 0.83 (95% CI 0.72–0.95). Compared to the STS PredDeep, predictions improved by 6.5% (IDI). However, both STS and REPINF had limited calibration. Different populations require independent scoring systems to achieve the best predictive effect. As the STS, the REPINF external validation across multiple centers it’s important to guide healthcare professionals as a quality improvement tool in the prevention of DSWI after CABG surgery.
Bioconversion of Crude Glycerol to Hydrogen and Methane in Three-Stage Horizontal Anaerobic Fixed-Bed Reactors (HARFB)
Authors: Adames Luan Vieira , Jacobus Ana Paula , Sakamoto Isabel , Lázaro Carolina Zampol , Pires Lorena Oliveira , Maintinguer Sandra Imaculada 
Published: November 2021

Abstract
The anaerobic conversion of residual glycerol from biodiesel production is an excellent option for producing clean energy at a low cost. Crude glycerol (CG) is an excellent carbon source for microorganisms in Anaerobic Digestion (AD) process; however, it is a nutrient-limited waste. On the other hand, domestic Sewage (DS) is wastewater containing macronutrients, such as nitrogen and phosphorus. Therefore, feeding bioreactors with a blend of both wastes is an exciting approach. The objective of this study was to operate a system consisting of three horizontal anaerobic series reactors (HARFB) with fixed bed (R1, R2, and R3), fed with a mixture of crude glycerol and domestic sewage to evaluate the start-up strategy and best organic loading rate (OLR) to improve biogas production (H2 e CH4) as well as to analyze the dynamic changes in the anaerobic microbial consortium during the operation of the HARFB. The highest hydrogen generation was 10.3 moles H2 (m3 d-1) in reactor R1, and the highest methane yield was 312.0 and 283.9 L CH4 (m3 d-1) in R2 and R3, respectively. The three-stage system showed high efficiency in removing crude glycerol and Chemical Oxygen Demand (COD), with consumptions of 99.9%. In addition, there was a change in the relative abundance of microorganisms amongst R1, R2, and R3 and a considerable decrease in the diversity index in the fermentation reactor (R1). The results have shown the potential of applying HARFB reactors for energy recovery and alternative waste disposal.
Efficacy of Early Respiratory Physiotherapy and Mobilization After on-pump Cardiac Surgery: a Prospective Randomized Controlled Trial
Authors: Afxonidis Georgios , Moysidis Dimitrios V. , Papazoglou Andreas S. , Tsagkaris Christos , Loudovikou Anna , Tagarakis Georgios , Karapanagiotidis Georgios T. , Alexiou Ioannis A. , Foroulis Christoforos , Anastasiadis Kyriakos 
Published: November 2021

Abstract
Objective: This randomized controlled trial aimed to investigate the influence of physical activity and respiratory physiotherapy on zero postoperative day on clinical, hemodynamic and respiratory parameters of patients undergoing cardiac surgeries under extracorporeal circulation.Methods: 78 patients undergoing coronary artery bypass graft (CABG) or/and valvular heart disease surgeries were randomly assigned into active physiotherapy group (APG; n=39) and conventional physiotherapy group (CPG; n=39). Treatment protocol for APG included ≤3 Mets of physical activity and respiratory physiotherapy on zero post-operative day and an extra physiotherapy session during the first three post-operative days, whereas CPG was treated with usual physiotherapy care after the first post-operative day. The length of hospital and intensive care unit (ICU) stay were set as the primary study outcomes, while pre- and post-intervention measurements were also performed to assess the oxymetric and hemodynamic influence of early mobilization and physiotherapy. Results: Participants’ mean age was 51.86 ±13.76 years. Of them 48 (61.5%) underwent CABG. Baseline and peri-procedural characteristics did not differ between the 2 groups. The total duration of hospital and ICU stay were significantly higher in the CPG compared to the APG (8.1±0.4 versus 10.2±0.6 days and 32.1±1.7 versus 23.2±0.6 hours, p<0.001, respectively). Statistically significant differences in pre-intervention oxygen saturation, and post-intervention PO2 and lactate levels were also observed between the 2 groups (p=0.022, 0.027 and 0.001, respectively). Conclusion: In on-pump cardiac surgery, early and active post-procedural physical activity (≤3 METS) can prevent prolonged ICU stay and decrease the duration of hospitalization whilst ameliorating post-operative hemodynamic and oxymetric parameters.
Gene Co-Expression Network Characterizing Microenvironmental Heterogeneity and Intercellular Communication in Pancreatic Ductal Adenocarcinoma: Implications of Prognostic Significance and Therapeutic Target
Authors: Wu Chengsi , Liu Yizhen , Cai Kun , Tao Li , Wei Dianhui , Jing Tiantian , Wang Boshi 
Published: November 2021

Abstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is characterized by intensive stroma involvement and heterogeneity. Pancreatic cancer cells interplay with surrounding tumor micro-environment (TME), leading to exacerbated tumorigenesis, dismal prognosis and tenacious therapy resistance. Herein, we aim to ascertain a gene-network indicative of vicious features of TME, then find a vulnerability for pancreatic cancer. Methods: Single cell RNA sequencing data was processed by Seurat package, retrieving the cell component marker genes (CCMGs). Correlation networks/modules of CCMGs were determined by WGCNA algorithm in a combined PDAC mRNA expression dataset. The gene modules that statistically associate with prognosis were chosen for classifying TME subgroups, constructing neural network and designing the risk score system. Cell-cell communication analysis was achieved by NATMI software. The tumor suppressive effect of ITGA2 inhibitor was evaluated in vivo by using a KrasG12D-driven murine pancreatic cancer model. Results: WGCNA analysis categorized cell component marker genes into eight co-expression networks. From gene modules with the maximum and minimum hazard ratio, we stratify PDAC samples based on TME gene patterns, resulting in two main TME subclasses with contrasting survival periods. Furthermore, we generated a neural network model and a risk score model which robustly predict prognosis and therapeutic outcomes. The hub genes in both gene modules were also gathered for functional enrichment analysis, elucidating a crucial role of cell communication-mediating integrins in TME associated PDAC malignancy. To perform a confirmatory experiment underpinning the significance of hub gene targeting, the mice with spontaneously developed pancreatic cancer were orally treated with an integrin inhibitor. The in vivo assays unraveled that pharmacologically inhibiting ITGA2 counteracts cancer-promoting micro-environment, and ameliorates pancreatic lesions. Conclusions: By recapitulating gene-network across various cell types, we exploited novel PDAC prognosis-predicting strategies. Medically interfering ITGA2, a key factor guiding cellular reciprocal interaction, attenuated tumor development. These findings may open new avenue about PDAC targeting therapy.