bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic review on ethical challenges of ‘field’ research in low-income and middle-income countries: respect, justice and beneficence for research staff?
Authors: Steinert Janina Isabel , Atika Nyarige David , Jacobi Milan , Kuhnt Jana , Kaplan Lennart 
Published: July 2021

IntroductionPrimary data collection in low-income and middle-income countries (LMICs) is associated with a range of ethical complexities. Considerations on how to adequately ensure the well-being of research staff are largely neglected in contemporary ethics discourse. This systematic review aims to identify the ethical challenges that research staff across different hierarchical levels and scientific disciplines face when conducting research in LMICs.MethodsWe searched 13 electronic databases and handsearched publications in six selected journals as well as the reference lists of all included studies. No restrictions were applied with respect to the publication date, research design, and target population.Results23 151 studies were retrieved, 183 of which met our inclusion criteria. We identified nine different types of ethical challenges that research staff may be exposed to during field research, including (1) role conflicts that can emerge from participants’ help requests and the high level of deprivation found in certain study settings, (2) feelings of guilt and (3) detrimental mental health impacts. Further challenges were (4) sexual harassment (5) safety risks and (6) political repression, particularly in postconflict, disaster-ridden or autocratic study contexts. Additionally, studies reported (7) inadequate working conditions and (8) power imbalances within research teams, while (9) ethics boards were found to be ill equipped to anticipate and address emerging risks, thus increasing the ethical liability of researchers.ConclusionThis review revealed several complex ethical challenges that research staff may face during data collection. In order to achieve the Sustainable Development Goal 8.8 on ‘safe and secure working environments’ and to protect research staff from harm, amendments must urgently be made to current ethical standards.PROSPERO registration numberCRD42019131013
Protocol for the economic evaluation of the China Salt Substitute and Stroke Study (SSaSS)
Authors: Li Ka-Chun , Tian Maoyi , Neal Bruce , Huang Liping , Yu Jie , Liu Yishu , Yin Xuejun , Zhang Xinyi , Wu Yangfeng , Li Nicole , Elliott Paul , Yan Lijing , Labarthe Darwin , Hao Zhixin , Shi Jingpu , Feng Xiangxian , Zhang Jianxin , Zhang Yuhong , Zhang Ruijuan , Zhou Bo , Li Zhifang , Sun Jixin , Zhao Yi , Yu Yan , Si Lei , Lung Thomas 
Published: July 2021

IntroductionCardiovascular diseases (CVDs) are the leading causes of death and disability worldwide. Reducing dietary salt consumption is a potentially cost-effective way to reduce blood pressure and the burden of CVD. To date, economic evidence has focused on sodium reduction in food industry or processed food with blood pressure as the primary outcome. This study protocol describes the planned within-trial economic evaluation of a low-sodium salt substitute intervention designed to reduce the risk of stroke in China.Methods and analysesThe economic evaluation will be conducted alongside the Salt Substitute and Stroke Study: a 5-year large scale, cluster randomised controlled trial. The outcomes of interest are quality of life measured using the EuroQol-5-Dimensions and major adverse cardiovascular events. Costs will be estimated from a healthcare system perspective and will be sought from the routinely collected data available within the New Rural Cooperative Medical Scheme. Cost-effectiveness and cost-utility analyses will be conducted, resulting in the incremental cost-effectiveness ratio expressed as cost per cardiovascular event averted and cost per quality-adjusted life year gained, respectively.Ethics and disseminationThe trial received ethics approval from the University of Sydney Ethics Committee (2013/888) and Peking University Institutional Review Board (IRB00001052-13069). Informed consent was obtained from each study participant. Findings of the economic evaluation will be published in a peer-reviewed journal and presented at international conferences.Trial registration numberClinicalTrials.gov Registry (NCT02092090).
Impact of dietary aflatoxin on immune development in Gambian infants: a cohort study
Authors: Xu Ya , Moore Sophie , Chen Gaoyun , Nshe Patrick , Faye-Joof Tisbeh , Prentice Andrew , Gong Yun Yun , Routledge Michael 
Published: July 2021

BackgroundChronic aflatoxin (AF) exposure has been shown to occur at high levels in children from sub-Saharan Africa (SSA), and has been associated with growth retardation and immune dysfunction. Our objective was to investigate the impact of AF exposure on immune development in early infancy using thymic size and antibody (Ab) response to vaccination as indicators of immune function.MethodsA total of 374 infants born between May 2011 and December 2012 were enrolled into the current study. These infants were recruited from a larger, randomised trial examining the impact of nutritional supplementation of mothers and infants on infant immune development (the Early Nutrition and Immune Development Trial). Thymic size (Thymic Index, TI) was measured by sonography at 1 week, 8 weeks, 24 weeks and 52 weeks of infant age. Infants were given the diphtheria–tetanus–pertussis (DTP) vaccine at 8 weeks, 12 weeks and 16 weeks of age, and Ab responses to each vaccine measured at 12 weeks and 24 weeks of age. AF-albumin (AF-alb) adduct levels in infant blood were measured by ELISA as the biomarker of AF exposure.ResultsThe geometric mean (GM) level of AF-alb increased with age. Only half of infants had detectable AF-alb with a GM of 3.52 pg/mg at 24 weeks, increasing to 25.39 pg/mg at 52 weeks, when 98% of infants had AF-alb >limit of detection. Significant negative association of AF-alb level with TI was seen in infants during the first 24 weeks, especially at 8 weeks of age (p<0.001), which is the time point of fastest thymus growth. There were no associations between AF exposure level and Ab response to pertussis and tetanus, but a significant positive correlation was observed between AF-alb level and Ab titre to diphtheria (p<0.005).ConclusionsHigh levels of AF exposure during early infancy may impact on infant immune development.Trial registration numberISRCTN49285450.
Household cooking fuel type and childhood anaemia in sub-Saharan Africa: analysis of cross-sectional surveys of 123, 186 children from 29 countries
Authors: Amadu Iddrisu , Seidu Abdul-Aziz , Afitiri Abdul-Rahaman , Ahinkorah Bright Opoku , Yaya Sanni 
Published: July 2021

ObjectiveThis study sought to investigate the joint effect of household cooking fuel type and urbanicity (rural–urban residency) on anaemia among children under the age of five in sub-Saharan Africa.DesignWe analysed cross-sectional data of 123, 186 children under the age of five from 29 sub-Saharan African countries gathered between 2010 and 2019 by the Demographic and Health Survey programme. Bivariate (χ2test of independence) and multilevel logistic regression were used to examine the effect of urbanicity-household cooking fuel type on childhood anaemia. Results were reported as adjusted odds ratios (aORs) with 95% CIs at p<0.05.Outcome measuresAnaemia status of children.ResultsMore than half (64%) of children had anaemia. The percentage of children who suffered from anaemia was high in those born to mothers in Western Africa (75%) and low among those born in Southern Africa (54%). Children from rural households that depend on unclean cooking fuels (aOR=1.120; 95% CI 1.033 to 1.214) and rural households that depend on clean cooking fuels (aOR=1.256; 95% CI 1.080 to 1.460) were more likely to be anaemic as compared with children from urban households using clean cooking fuel. Child’s age, sex of child, birth order, perceived birth size, age of mother, body mass index of mother, education, marital status, employment status, antenatal care, wealth quintile, household size, access to electricity, type of toilet facility, source of drinking water and geographic region had significant associations with childhood anaemia status.ConclusionsOur study has established a joint effect of type of household cooking fuel and urbanicity on anaemia among children under the age of five in sub-Saharan Africa. It is therefore critical to promote the usage of clean cooking fuels among households and women in rural areas. These should be done taking into consideration the significant child, maternal, household, and contextual factors identified in this study.
Prevalence of childhood mental disorders in high-income countries: a systematic review and meta-analysis to inform policymaking
Authors: Barican Jenny Lou , Yung Donna , Schwartz Christine , Zheng Yufei , Georgiades Katholiki , Waddell Charlotte 
Published: July 2021

QuestionMental disorders typically start in childhood and persist, causing high individual and collective burdens. To inform policymaking to address children’s mental health in high-income countries we aimed to identify updated data on disorder prevalence.MethodsWe identified epidemiological studies reporting mental disorder prevalence in representative samples of children aged 18 years or younger—including a range of disorders and ages and assessing impairment (searching January 1990 through February 2021). We extracted associated service-use data where studies assessed this. We conducted meta-analyses using a random effects logistic model (using R metafor package).FindingsFourteen studies in 11 countries met inclusion criteria, published from 2003 to 2020 with a pooled sample of 61 545 children aged 4–18 years, including eight reporting service use. (All data were collected pre-COVID-19.) Overall prevalence of any childhood mental disorder was 12.7% (95% CI 10.1% to 15.9%; I2=99.1%). Significant heterogeneity pertained to diagnostic measurement and study location. Anxiety (5.2%), attention-deficit/hyperactivity (3.7%), oppositional defiant (3.3%), substance use (2.3%), conduct (1.3%) and depressive (1.3%) disorders were the most common. Among children with mental disorders, only 44.2% (95% CI 37.6% to 50.9%) received any services for these conditions.ConclusionsAn estimated one in eight children have mental disorders at any given time, causing symptoms and impairment, therefore requiring treatment. Yet even in high-income countries, most children with mental disorders are not receiving services for these conditions. We discuss the implications, particularly the need to substantially increase public investments in effective interventions. We also discuss the policy urgency, given the emerging increases in childhood mental health problems since the onset of the COVID-19 pandemic (PROSPERO CRD42020157262).
Next-generation sequencing of bile cell-free DNA for the early detection of patients with malignant biliary strictures
Authors: Arechederra Maria , Rullán María , Amat Irene , Oyon Daniel , Zabalza Lucia , Elizalde Maria , Latasa M Ujue , Mercado Maria R , Ruiz-Clavijo David , Saldaña Cristina , Fernández-Urién Ignacio , Carrascosa Juan , Jusué Vanesa , Guerrero-Setas David , Zazpe Cruz , González-Borja Iranzu , Sangro Bruno , Herranz Jose M , Purroy Ana , Gil Isabel , Nelson Leonard J , Vila Juan J , Krawczyk Marcin , Zieniewicz Krzysztof , Patkowski Waldemar , Milkiewicz Piotr , Cubero Francisco Javier , Alkorta-Aranburu Gorka , G Fernandez-Barrena Maite , Urman Jesus M , Berasain Carmen , Avila Matias A 
Published: July 2021

ObjectiveDespite significant progresses in imaging and pathological evaluation, early differentiation between benign and malignant biliary strictures remains challenging. Endoscopic retrograde cholangiopancreatography (ERCP) is used to investigate biliary strictures, enabling the collection of bile. We tested the diagnostic potential of next-generation sequencing (NGS) mutational analysis of bile cell-free DNA (cfDNA).DesignA prospective cohort of patients with suspicious biliary strictures (n=68) was studied. The performance of initial pathological diagnosis was compared with that of the mutational analysis of bile cfDNA collected at the time of first ERCP using an NGS panel open to clinical laboratory implementation, the Oncomine Pan-Cancer Cell-Free assay.ResultsAn initial pathological diagnosis classified these strictures as of benign (n=26), indeterminate (n=9) or malignant (n=33) origin. Sensitivity and specificity of this diagnosis were 60% and 100%, respectively, as on follow-up 14 of the 26 and eight of the nine initially benign or indeterminate strictures resulted malignant. Sensitivity and specificity for malignancy of our NGS assay, herein named Bilemut, were 96.4% and 69.2%, respectively. Importantly, one of the four Bilemut false positives developed pancreatic cancer after extended follow-up. Remarkably, the sensitivity for malignancy of Bilemut was 100% in patients with an initial diagnosis of benign or indeterminate strictures. Analysis of 30 paired bile and tissue samples also demonstrated the superior performance of Bilemut.ConclusionImplementation of Bilemut at the initial diagnostic stage for biliary strictures can significantly improve detection of malignancy, reduce delays in the clinical management of patients and assist in selecting patients for targeted therapies.
Effect of whole-body resistance training at different load intensities on circulating inflammatory biomarkers, body fat, muscular strength, and physical performance in postmenopausal women
Authors: Carneiro Marcelo A.S. , Oliveira Júnior Gersiel N. de , de Sousa Jairo F.R. , Orsatti Claudio L. , Murta Eddie F.C. , Michelin Márcia A. , Cyrino Edilson S. , Orsatti Fábio L. 
Published: July 2021

The primary purpose of this study was to identify the impact of whole-body resistance training (RT) at different load intensities on adipokines, adhesion molecules, and extracellular heat shock proteins in postmenopausal women. As secondary purpose, we analyzed the impact of RT at different load intensities on body fat, muscular strength, and physical performance. Forty participants were randomized into lower-load intensity RT (LIRT, n = 20, 30–35 repetition maximum in the first set of each exercise) or higher-load intensity RT (HIRT, n = 20, 8–12 repetition maximum in the first set of each exercise). Adipokines (adiponectin and leptin), adhesion molecules (MCP-1 and ICAM-1), extracellular heat shock proteins (HO-1 and eHSP60), body fat, muscular strength (1RM), and physical performance [400-meter walking test (400-M) and 6-minute walking test (6MWT)] were analyzed at baseline and after 12-weeks RT. There was a significant time-by-group interaction for eHSP60 (P = 0.049) and 400-M (P = 0.003), indicating superiority of HIRT (d = 0.47 and 0.55). However, both groups similarly improved adiponectin, ICAM-1, HO-1, body fat, 1RM, and 6MWT (P < 0.05). Our study suggests that load intensity does not seem to determine the RT effect on several obesity-related pro-inflammatory and chemotactic compounds, body fat, 1RM, and 6MWT in postmenopausal women, although a greater improvement has been revealed for eHSP60 and 400-M in HIRT. Novelty: Higher-load intensity resistance training improves eHSP60 and 400-M in postmenopausal women. Resistance training improves the inflammatory profile, body fat, muscle strength, and 6MWT, regardless of load intensity.
Dlx5promotes cancer progression through regulation ofCCND1in oral squamous cell carcinoma (OSCC)
Authors: Zhang Jianfei , Wu Jinyang , Chen Yang , Zhang Wenbin 
Published: July 2021

Genetic studies have revealed a critical role of the distal-less homeobox gene 5 (Dlx5) in the pathogenesis of ovarian cancer, lung cancer, and T-cell lymphoma; however, the role and underlying mechanisms of Dlx5 in oral squamous cell carcinoma (OSCC) are largely unknown. In this study, we demonstrated that Dlx5 is up-regulated in OSCC tissues and cell lines, compared with their control groups. The results from our immunohistochemistry (IHC) analyses show that high expression levels of Dlx5 correlated with advanced TNM stages (P = 0.0001), lymph node metastasis (P = 0.0049), poor cellular differentiation (P = 0.0491), location of the tumors (P = 0.0132), and poor prognosis for the patient. We also demonstrated that knockdown of Dlx5 inhibited the viability, proliferation, and colony formation of OSCC cell lines CAL-27 and WSU-HN6 cells, probably by blocking cell cycle in the G1 phase. Furthermore, we revealed that Dlx5 exerts its biological functions via direct regulation of CCND1 in CAL-27 and WSU-HN6 cells. Ultimately, we have demonstrated that silencing of Dlx5 inhibits the growth of xenograft tumors in vivo, and that Dlx5 affects the progression of OSCC both in vitro and in vivo via directly regulating CCND1, providing a potential diagnostic biomarker and therapeutic target for OSCC.
Effect of oregano oil and cranberry pulp supplementation in finishing pigs on the physicochemical quality of fresh loin during storage
Authors: Fortier M.P. , Guay F. , Saucier L. 
Published: July 2021

Oregano oil and cranberry pulp supplements were added to the diets of finishing pigs to determine their effects on the meat quality of fresh loins during storage. Two and three levels of oregano oil (250 and 500 mg·kg−1) and cranberry pulp (5, 10, and 20 g·kg−1) were tested, according to a factorial experimental design. The loin meat was vacuum packed and analyzed at 0 (after the 24 h chilling period post slaughter), 23, 45, and 60 d of storage. Samples were repackaged under aerobic conditions after 0 or 23 d and analyzed after 4, 8, and 12 d. Oregano and cranberry supplements did not affect lipid oxidation (microgram of malondialdehyde equivalent per kilogram of meat) during anaerobic or aerobic storage. On day 0, the fatty acid profile of the loin samples demonstrated that the addition of cranberries at a dose of 10 g·kg−1 was associated with a lower percentage of saturated fatty acids (P = 0.04; 42.97% vs. 40.99%) and a trend for a higher percentage of monounsaturated fatty acids (P = 0.06; 47.26% vs. 46.09%). Considering the result obtained, feeding pigs with oregano and cranberry supplements had a limited effect on meat quality parameters measured during storage.
Fluctuations and phases in baryonic matter
Authors: Brandes Len , Kaiser Norbert , Weise Wolfram 
Published: July 2021

AbstractThe phase structure of baryonic matter is investigated with focus on the role of fluctuations beyond the mean-field approximation. The prototype test case studied is the chiral nucleon-meson model, with added comments on the chiral quark-meson model. Applications to nuclear matter include the liquid-gas phase transition. Extensions to high baryon densities are performed for both nuclear and neutron matter. The role of vacuum fluctuations is systematically explored. It is pointed out that such fluctuations tend to stabilize the hadronic phase characterized by spontaneously broken chiral symmetry, shifting the chiral restoration transition to very high densities. This stabilization effect is shown to be further enhanced by additional dynamical fluctuations treated with functional renormalisation group methods.
Test of gravitational red-shift based on tri-frequency combination of microwave frequency links
Authors: Sun Xiao , Shen Wen-Bin , Shen Ziyu , Cai Chenghui , Xu Wei , Zhang Pengfei 
Published: July 2021

AbstractOver the decades, testing gravitational red-shift (GRS) based on microwave links has made great process, including the GPA experiment, the planned Atomic Clock Ensemble in Space mission, and the China Space Station (CSS). Until now, the formulations of microwave links are almost all based on the time comparison. However, there are advantages of using frequency comparison instead of time comparison to test GRS. Here we develop a tri-frequency combination method based on the measurements of the frequency shifts of three independent microwave links between a space station and a ground station. Aiming at the frequency links’ accuracy of $$3\times 10^{-16}$$

3
×

10

-
16

, we should consider various effects, including the Doppler effect, second-order Doppler effect, atmospheric frequency shift, tidal effects, refraction caused by the atmosphere, and Shapiro effect, with accuracy levels of tens of centimeters. The CSS will complete construction in 2022, and the formulation proposed in this study will enable us to test GRS at an accuracy level of at least $$2\times 10^{-6}$$

2
×

10

-
6

, which is one order higher than the present accuracy level of $$7\times 10^{-5}$$

7
×

10

-
5

.
Prospecting black hole thermodynamics with fractional quantum mechanics
Authors: Jalalzadeh S. , da Silva F. Rodrigues , Moniz P. V. 
Published: July 2021

AbstractThis paper investigates whether the framework of fractional quantum mechanics can broaden our perspective of black hole thermodynamics. Concretely, we employ a space-fractional derivative (Riesz in Acta Math 81:1, 1949) as our main tool. Moreover, we restrict our analysis to the case of a Schwarzschild configuration. From a subsequently modified Wheeler–DeWitt equation, we retrieve the corresponding expressions for specific observables. Namely, the black hole mass spectrum, M, its temperature T, and entropy, S. We find that these bear consequential alterations conveyed through a fractional parameter, $$\alpha $$
α
. In particular, the standard results are recovered in the specific limit $$\alpha =2$$

α
=
2

. Furthermore, we elaborate how generalizations of the entropy-area relation suggested by Tsallis and Cirto (Eur Phys J C 73:2487, 2013) and Barrow (Phys Lett B 808:135643, 2020) acquire a complementary interpretation in terms of a fractional point of view. A thorough discussion of our results is presented.
Euploid Day-5 Blastocysts Versus Euploid Day-6 Blastocysts — Will the Reproductive Outcomes Differ? An Observational Study
Authors: Rao Durga Gedela , Mantravadi Krishna Chaitanya , Sharanappa Vijay Kumar 
Published: July 2021

Background and objective: Day-5 blastocyst embryos are usually chosen for assisted reproductive therapy. We compared the reproductive outcomes of the euploid blastocysts developed on Day 5 versus Day 6. Methods: This single-center, retrospective observational study analyzed patients aged 25–45 years, who underwent intracytoplasmic sperm injection from December 2014 to November 2018. Depending on the day of trophectoderm biopsy, patients were categorized into Day-5 and Day-6 groups. Percentages of euploid embryos were calculated for both groups, and elective single euploid blastocysts were transferred in a frozen embryo transfer (FET) cycles. The study endpoints were the comparisons of the reproductive outcomes including clinical pregnancy rate (CPR), implantation rate (IR), miscarriage rate (MR), and live birth rate (LBR) between Day-5 and Day-6 euploid FET groups. Results: A total of 801 embryos from 184 patients were evaluated [Day 5 ([Formula: see text]); Day 6 ([Formula: see text]); 42.45% were euploid] with the rate of euploidy in Day-5 and Day-6 groups at 42.52% and 40.62%, respectively. A total of 126 patients underwent FET with 126 elective single euploid embryos (Day 5: 117; Day 6: 9). For Day-5 versus Day-6 groups, a significantly higher IR (61.54% vs. 44.44%; [Formula: see text]), CPR (61.54% vs. 44.44%; [Formula: see text]), and LBR (61.54% vs. 33.33%; [Formula: see text]) were reported. Multivariate analysis on ANOVA suggested, comparable pregnancy rates at Day 5 and Day 6 ([Formula: see text]). Conclusions: Day-5 euploid blastocysts seem to offer better reproductive outcomes than Day-6 euploid blastocysts. Further research is recommended to evaluate the reproductive outcomes of Day-6 blastocysts.