bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co‐Expression of Dominant‐Negative TGF‐β Receptor 2 Enhances the Therapeutic Efficacy of Novel TREM1/DAP12‐BB‐Based CAR‐T Cells in Solid Tumours
Authors: Zhu Sichao , Hu Jianping , Lin Jie , Wang Chen , Wang Enxiu 
Published: January 2025

ABSTRACTChimeric antigen receptor (CAR) T‐cell therapy has exhibited remarkable efficacy in the treatment of haematological malignancies, yet its application in solid tumours is hindered by the immunosuppressive tumour microenvironment (TME). In this study, a novel SS1‐TREM1/DAP12‐BB CAR‐T cell was devised to target ovarian cancer and further engineered to co‐express the dominant‐negative TGF‐β receptor 2 (DNR) to combat CAR‐T cell exhaustion in TME. The incorporation of DNR effectively blocked TGF‐β signalling, thereby enhancing CAR‐T cell survival and antitumor activity in a TGF‐β1‐rich environment. In vivo evaluations demonstrated that DNR co‐expression potentiated the antitumor efficacy of TREM1/DAP12‐BB CAR‐T cells and conferred resilience against tumour rechallenge. These findings underscore the broad potential of DNR co‐expression in CAR design, presenting a novel therapeutic strategy for patients with recurrent ovarian cancer.
Characterisation of Water and Heat Fluxes of Typical Crops and Simulation of Evapotranspiration in Saline‐Alkali Soil of the Yellow River Delta, China
Authors: Haonan Qiu , Shihong Yang , Guangmei Wang , Xiaoling Liu , Jie Zhang , Yi Xu , Shide Dong , Hanwen Liu , Zewei Jiang 
Published: January 2025

ABSTRACTThe investigation of water and heat flux variation patterns in saline‐alkali land is significant due to the distinctive characteristics that affect crop growth, and surface energy flux and evapotranspiration are two key factors affecting saline‐alkali land. Surface energy fluxes and evapotranspiration of three crops (wheat, maize, and soybean) in saline‐alkali soils were observed using an EC (eddy covariance) system. The energy balance closure of the three crops was evaluated at the daily scale with regression slopes of 0.82 for wheat, 0.83 for maize, and 0.65 for soybean. During the growing season, wheat, maize, and soybean exhibited average LE (latent heat) to Rn (net radiation) ratios of 0.66, 0.55, and 0.65, respectively. Notably, LE dominated the consumption of Rn. The correlation analysis showed that the three crops in saline‐alkali soil had the highest correlation with Rn and photosynthetic photon flux density (PPFD) and a negative correlation with humidity (RH). Notably, crops in saline‐alkali soil exhibited more pronounced nocturnal evapotranspiration (ET) variations in the middle and late growth stages compared to other dryland cropping systems. Based on the observed ET data and meteorological factors, this paper constructed ET prediction models for three crops in saline‐alkali soil at 30‐min and daily scales using back propagation neural network (BP), random forest (RF), and k‐neighbourhood (KNN). BP exhibits better model performance. In most cases, the results showed that the best model performance of wheat, maize, and soybeans at the 30‐min scale were R2 = 0.812, RMSE = 0.0449 mm; R2 = 0.683, RMSE = 0.0858 mm; R2 = 0.802, RMSE = 0.0672 mm. The work in this study contributes to the understanding of the changing patterns of water–heat fluxes in crops in saline‐alkali soil and enables prediction of crop evapotranspiration in saline soils.
Integrated experimental and thermodynamic modeling study of phase equilibria in the PbO‐CaO‐AlO1.5 system in air
Authors: Khartcyzov Georgii , Shevchenko Maksym , Nekhoroshev Evgenii , Jak Evgueni 
Published: January 2025

AbstractThe present study focused on the phase equilibria in the PbO‐CaO‐AlO1.5 and PbO‐CaO systems in air. The high‐temperature phase equilibria in these systems were studied using the high‐temperature equilibration and quenching technique. The compositions of phases present in the quenched samples were then measured using the electron probe X‐ray microanalysis. The liquidus of the PbO‐CaO‐AlO1.5 system in air, including Ca3Al2O6, Ca12Al14O33, (Ca,Pb)Al2O4, CaAl4O7, (Ca,Pb)Al12O19, Ca2PbO4, corundum (Al2O3), lime (CaO), massicot (PbO), Pb9Al8O21, PbAl2O4, and PbCa2Al8O15 primary phase fields as well as the immiscibility gap in the liquidi of Ca3Al2O6, Ca12Al14O33, and (Ca,Pb)Al2O4 have been experimentally characterized. The experimental data obtained were used for the thermodynamic optimization of a self‐consistent set of parameters of the thermodynamic model for all phases of the PbO‐CaO‐AlO1.5 system in air, in agreement with the Cu‐Pb‐Zn‐Fe‐Ca‐Si‐Al‐Mg‐O‐S‐(As, Sn, Sb, Bi, Ag, Au, Ni, Cr, Co, and Na) gas/oxide liquid/matte/speiss/metal/solids system in support of the development and optimization of pyrometallurgical processes. The predicted liquidus projection of the PbO‐CaO‐AlO1.5 system was presented for the first time in the complete range of temperatures and compositions.
Exploring the Impact of Thermal Processing on the Quality Attributes of Honey: A Comprehensive Review
Authors: Bhure Rohan Avinash , Alam Masud , Nanda Vikas , Pawar Vikas M. , Saxena Sachin 
Published: January 2025

ABSTRACTNectar collected by bees from diverse origins, at different time points throughout the year, under different environmental conditions, and from diverse flora influences the composition of the honey. However, intensive migratory beekeeping practices and unhygienic extraction methods can compromise its aesthetic appeal, nutritional quality, and microbiological safety. Thermal treatment is a critical step in industrial honey processing, aimed at extending shelf life by reducing moisture content, improving viscosity for efficient handling, transportation, and packaging. Despite these advantages, thermal processing can adversely affect honey's quality by increasing hydroxymethylfurfural (HMF) levels and reducing diastase activity, both key indicators of honey's freshness and quality. Although typical HMF levels in honey are generally considered safe, concerns about its potential carcinogenicity in specific food items, though minimal, underscore the need for careful processing. This review comprehensively examines the effects of thermal treatments on honey's quality attributes, including its nutritional, sensory, and microbiological properties. It also highlights strategies to moderate the negative impacts of heat treatment, ensuring the production of high‐quality honey with lower HMF content and preserved bioactivity. Future research directions are discussed to optimize processing techniques, addressing safety concerns while maintaining honey's unique biological and functional properties.
Effects of a self‐efficacy‐centered self‐management program on neurogenic bladder after spinal cord injury: A randomized controlled trial
Authors: Tang Xiangxiang , Liu Qian , Su Xiaoping , Yu Zepeng 
Published: January 2025

AbstractPurposeThe present research aimed to investigate the effects of a self‐efficacy‐centered self‐management program on persons with neurogenic bladder (NGB) after spinal cord injury (SCI).DesignA randomized controlled trial.Materials and MethodsEighty‐two individuals with NGB after spinal cord injury (SCI) were randomized into either the control or intervention group. The control group received standard admission and discharge education and follow‐up. The intervention group received the self‐efficacy‐centered self‐management program. Self‐efficacy was assessed at preintervention, 4, 8, and 12 weeks post‐intervention. Self‐management ability, the incidence of urinary tract infection (UTI), residual urine volume, bladder safety capacity, and quality of life were assessed at 12 weeks post‐intervention.ResultsOf the 82 recruited participants, only 79 participants (control group: n = 39, 67% males, intervention group: n = 40, 58% males) received the full intervention. A significant difference was observed in self‐efficacy score between the control and intervention groups (2.54 ± .247 versus 2.71 ± .218, mean difference = .174, 95% confidential interval: .070–.278, p = .001) at 12 weeks post‐intervention. The intervention group demonstrated significantly better outcomes than the control group at 12 weeks post‐intervention in self‐management ability, urinary tract infection (UTI) incidence, residual urine volume and bladder safety capacity. There were significant differences in quality of life (vitality [VT] and mental health [MH] subscales) between the two groups.ConclusionsThe self‐efficacy‐centered self‐management program developed in this study can significantly increase participants' self‐efficacy and bladder self‐management ability, thereby improving their bladder functional status and quality of life.
Sustainable Sociability: Allowing Sociability Through Door Design in the Age of Social Media, a Practical Exploration of Modularity With Traditional Techniques
Authors: Hafeez Xahra , Hafeez Sana 
Published: January 2025

ABSTRACTThis research presents the development of innovative modular doors specifically designed to foster interpersonal connections in digital environment of today. The door is analyzed both as a symbol and as a site of projective identification, exploring how it represents emotional or psychological states and how people may project their identity onto it. The present analysis looks at thresholds and doors in cultural anthropology and how they impact human behaviors due to their shape, form and function in variety of cultures and different time periods. The second aspect of the research deals with the designing of modular doors which cater to our current social needs. These doors create opportunities by integrating flexible, open designs that reduce acoustic and visual barriers for spontaneous interactions. Making social engagement more natural and frequent while keeping a balance between connectivity and privacy. The findings suggest that such designs can play a crucial role in promoting more meaningful face‐to‐face interactions. It also brings the focus back from industrial and mass‐produced products to handmade pieces by using sustainable materials, locally sourced and crafted by artisans in Pakistan.
From Hopeful Heroes to Cynical Martyrs: Identity Work and the Path‐Dependent Identification with Maladaptive Logics
Authors: Botha Lindie , Hamann Ralph 
Published: January 2025

AbstractScholars have long attended to both the persistence and change of institutional logic–identity constellations, but we know less about why and how organizational members might cling to a logic despite its evident maladaptive character and the resulting emotional upheaval. Based on a 5‐year ethnography of a conservation organization's paramilitary campaign against rhino poaching, we induct a process model to show how the crisis‐induced adoption of a new logic and the corresponding identity work can have path‐dependent effects that tip hopeful heroism into cynical martyrdom and a dogged commitment to a maladaptive logic, with negative organizational implications. We identify three forms of identity work that act as self‐reinforcing mechanisms of this path dependence: polarizing, normalizing, and cynical coping. Elaborating the intersection of scholarship on institutions, identity work, identification, and path dependence, we explain how an initially valorized identity can twist into a darker, dysfunctional version of itself, with path‐dependent mechanisms contributing to organizational rigidity in the face of crises.
Desperate Journeys to Europe: Sensebreaking in Extreme Contexts
Authors: Chaudhry Amna , Amis John M. 
Published: January 2025

AbstractThis paper uncovers the ways in which sensebreaking processes are initiated and then unfold over time in extreme contexts. Using semi‐structured interviews with irregular migrants from Pakistan undertaking perilous journeys to Europe, this research makes two major contributions to the literatures on extreme contexts and sensebreaking. First, we develop two temporal modes of sensebreaking: sensebreaking with the past and sensebreaking with the future. We reveal the role of liminal spaces in sensebreaking and explain why sensebreaking needs to be reconceptualized as an inherently protracted, often indeterminate, process. Second, we uncover the triggers and drivers of sensebreaking in extreme contexts. We identify two states that precipitate sensebreaking, derealization and disorientation. We theorize the ways in which they are triggered by emotional and physical disruptions and their role in sustaining sensebreaking over time. We also explicate how temporal irregularities, particularly time contortion and time appropriation, influence the development of sensebreaking in extreme contexts.
Validation of the Morningness–Eveningness Scale for Children (MESC) with ambulatory circadian monitoring of temperature, light exposure and activity
Authors: Puig‐Navarro Yaiza , Díaz‐Morales Juan F. 
Published: January 2025

SummaryThe external validity of the Morningness–Eveningness Scale for Children was analysed via objective measures of skin temperature, light exposure and motor activity with the ambulatory circadian monitoring methodology. A total of 138 adolescents (57 boys and 81 girls) aged 12–13 years, who in addition to completing the Morningness–Eveningness Scale for Children to determine their circadian typology wore a wrist activity device (Kronowise 3.0; Kronohealth SL) during school days and a weekend, participated. Circadian parameters, such as mesor, amplitude and acrophase, were estimated for skin temperature, light exposure and motor activity, as were sleep parameters, such as risetime, bedtime and social jetlag. The results indicated that during the weekend E‐type adolescents experienced later acrophases in temperature, light and activity than I‐type and M‐type adolescents did, whereas boys experienced earlier acrophases in temperature and activity. When school weekdays were compared with the weekend, there was a weekend delay in the acrophases of temperature (1:03), light exposure (2:03) and activity (3:15). The results obtained in this study provide external validity for applying the Morningness–Eveningness Scale for Children in the naturalistic context of high school while considering sex and type‐of‐day differences as important variables in chronopsychological studies.
Identity, Collective Action and Inaction: The Role of Self‐Esteem and Psychological Benefits
Authors: Shultziner Doron 
Published: January 2025

ABSTRACTThis paper proposes a theoretical framework of collective action that integrates three aspects of identity—social identity, moral convictions, and self‐esteem. The paper explains how these identity aspects relate to each other and how they affect collective action or inaction. The paper presents a framework that returns to and focuses on the underlying cognitive motivations to engage in collective action while paying special attention to the mechanism of maintaining positive self‐esteem and the benefits that people may gain from participation in collective action. These benefits explain both the personal incentives for action or inaction and the positive feedback loops of action on self‐esteem which reinforce additional mobilization and explain why some individuals participate more than their peers. The framework draws from existing theories and empirical studies to formulate testable hypotheses and stresses the importance of studying both participants and non‐participants of the same affected social group.
Optimization of the Use of APRI and FIB‐4 for Ruling Out Liver Cirrhosis in Chronic Hepatitis B Patients With Normal Alanine Aminotransferase
Authors: Zhang Zhiyi , Wang Jian , Zhu Li , Li Yiguang , Zhang Shaoqiu , Pan Yifan , Chen Yuxin , Yin Shengxia , Yan Xiaomin , Liu Xingxiang , Qiu Yuanwang , Wu Chao , Li Jie , Zhu Chuanwu , Huang Rui 
Published: January 2025

ABSTRACTThe exclusion of cirrhosis is important in chronic hepatitis B (CHB) patients with normal alanine aminotransferase (ALT). We aimed to optimise the performance of the aspartate aminotransferase to platelet ratio index (APRI) and fibrosis score based on four factors (FIB‐4) to exclude cirrhosis in these patients. Five hundred and eighty four patients with normal ALT who underwent liver biopsy were included in the study. The patients were divided into derivation and external validation sets. A grid search method was used to identify new cut‐offs with a negative predictive value (NPV) of > 95% and a sensitivity of > 90% for detecting cirrhosis. The proportion of patients with cirrhosis in the derivation and validation sets was 19.4% and 7.5%, respectively. The conventional cut‐offs of APRI (77.6%) and FIB‐4 (41.8%) had high rates of cirrhosis misclassification. A new APRI cut‐off of 0.21 had a sensitivity of 97.0% and an NPV of 95.6%, and only two (3.0%) patients with cirrhosis were misclassified in the derivation set. Using a new FIB‐4 cut‐off of 0.53, with a sensitivity of 98.5% and NPV of 96.2%, only one (1.5%) patient with cirrhosis was misclassified. External validation showed similar results. Using the new cut‐offs of APRI and FIB‐4, cirrhosis could be completely excluded for HBeAg‐positive patients or those aged > 40 years. The conventional cut‐offs had high misclassification rates for cirrhosis. The new cut‐offs of APRI (≤ 0.21) and FIB‐4 (≤ 0.53) could be used to exclude cirrhosis in CHB patients with normal ALT levels with a low misclassification rate.
Development and Evaluation of SYBR Green Real‐Time PCR for Rapid and Specific Identification of Trichophyton indotineae
Authors: Rouhi Faezeh , Aboutalebian Shima , Rezaei‐Matehkolaei Ali , Jahanshiri Zahra , Shidfar Mohammad‐Reza , Chadeganipour Amir‐Shayan , Shadzi Shahla , Kharazi Mahboobeh , Erami Mahzad , Mirhendi Hossein 
Published: January 2025

ABSTRACTBackgroundSince 2017, dermatophytosis caused by the newly introduced species Trichophyton indotineae has gained new interest worldwide due to the rise in terbinafine resistance and difficulty in the treatment of recalcitrant infections. Distinguishing T. indotineae from other Trichophyton species based on morphological features is impossible and DNA sequencing is necessary for accurate identification. Though early identification of the species is not solely sufficient for the treatment of infected cases, it is important for clinicians to take the next appropriate modalities such as antifungal susceptibility testing especially when the patients have extensive skin lesions recalcitrant to therapy by terbinafine. Here, we developed a rapid diagnostic scheme using SYBR Green real‐time PCR for the specific detection/identification of T. indotineae.MethodsDNA was extracted from 397 dermatophyte isolates and two SYBR Green real‐time PCR assays targeting the C120‐287 and E054‐58 intergenic loci were developed. Using a collection of 132 T. indotineae and 128 non‐T. indotineae strains, all had already been identified by ITS‐PCR‐sequencing and 137 unknown dermatophyte isolates, the assays were evaluated.ResultsIn both real‐time PCR assays, 130 out of 132 T. indotineae strains were positive while all non‐T. indotineae species were negative. Among 137 unknown tested isolates, 72 were identified as T. indotineae based on two real‐time PCR assays, while 65 showed no peak and were considered non‐T. indotineae. Based on PCR‐sequencing as the reference standard, the SYBR Green real‐time PCR assays demonstrated a sensitivity of 98.48% and a specificity of 100%.ConclusionThe developed diagnostic assays using SYBR Green real‐time PCR provided a rapid and accurate method for the distinction of cultured T. indotineae isolates and can be considered to evaluate for the detection of T. indotineae directly from clinical samples.
Audio‐visual concert performances synchronize audience's heart rates
Authors: Czepiel Anna M. , Fink Lauren K. , Scharinger Mathias , Seibert Christoph , Wald‐Fuhrmann Melanie , Kotz Sonja A. 
Published: January 2025

AbstractPeople enjoy engaging with music. Live music concerts provide an excellent option to investigate real‐world music experiences, and at the same time, use neurophysiological synchrony to assess dynamic engagement. In the current study, we assessed engagement in a live concert setting using synchrony of cardiorespiratory measures, comparing inter‐subject, stimulus–response, correlation, and phase coherence. As engagement might be enhanced in a concert setting by seeing musicians perform, we presented audiences with audio‐only (AO) and audio‐visual (AV) piano performances. Only correlation synchrony measures were above chance level. In comparing time‐averaged synchrony across conditions, AV performances evoked a higher inter‐subject correlation of heart rate (ISC‐HR). However, synchrony averaged across music pieces did not correspond to self‐reported engagement. On the other hand, time‐resolved analyses show that synchronized deceleration‐acceleration heart rate (HR) patterns, typical of an “orienting response” (an index of directed attention), occurred within music pieces at salient events of section boundaries. That is, seeing musicians perform heightened audience engagement at structurally important moments in Western classical music. Overall, we could show that multisensory information shapes dynamic engagement. By comparing different synchrony measures, we further highlight the advantages of time series analysis, specifically ISC‐HR, as a robust measure of holistic musical listening experiences in naturalistic concert settings.
Tripartite Symbiosis Between Legumes, Arbuscular Mycorrhizal Fungi and Nitrogen Fixing Rhizobia: Interactions and Regulation
Authors: Gorgia Polyxeni , Tsikou Daniela 
Published: January 2025

ABSTRACTLegume plants can interact with nitrogen‐fixing rhizobia bacteria and arbuscular mycorrhizal fungi (AMF) simultaneously, forming a tripartite symbiotic association. Co‐inoculation studies performed on a variety of legumes have shown that rhizobia and AMF influence each other when they co‐occur in tripartite association and affect host plant nutrition and performance. Although single plant‐microbe interactions have been extensively studied, our understanding in the field of tripartite interactions is insufficient and current knowledge cannot predict the symbiotic outcome, which appears to depend on many parameters. In this review we examine the current state of research on the legume‐rhizobium‐AMF tripartite symbiosis. We investigate the dynamic interaction between the two microsymbionts and the effect of one microbe on the other, both at the physiological and the molecular levels, and the result of dual inoculation on host plant growth, fitness and response to stresses. Rhizobia and AMF interact both extraradically and intraradically, effects on microbe and host plant gene expression levels are observed, AMF positively regulates nodulation, while rhizobia can affect AMF root colonisation either positively or negatively. Factors observed to regulate the establishment and function of the tripartite symbiosis, such as the rhizobia‐AMF combination, host plant identity and environmental conditions are discussed.