bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bilateral Charcot Arthropathy Treated with Bolt-Beam Constructs in the Setting of Unilateral Foot Ulcers
Authors: Sims Michael , Foropoulos Lauren , Bettin Clayton 
Published: July 2021

Case:
Two type-2 diabetic patients presented with bilateral Charcot arthropathy with midfoot collapse and presence of foot ulcerations on one side. The ulcerated extremity in each patient was treated surgically with fusion bolt, beam, and plate constructs in the setting of open ulceration. Bilateral extremities were treated surgically and went on to bony union and wound healing.

Conclusion:
Charcot arthropathy with open ulcers can be managed acutely with debridement, resection of bone, and arthrodesis. Waiting for ulcers to heal before internal fixation is performed may not be necessary in all situations.
THE INFLUENCE OF HOLISTIC FEED ON PERFORMANCE OF SUPER NATIVE CHICK
Authors: Supriyanto Supriyanto , Widiarso Budi Purwo , Sucipto Sucipto , Mentari Fabiana 
Published: July 2021

This study determines effects of raw material feed (derived from corn, bran, soybean pods, concentrates and Indigofera zollingeriana local to Magelang) on the performance of native chicks. Performance was measured by feed consumption, body weight gain, Feed Conversion Ratio (FCR), mortality, and morbidity. This experiment used Completely Randomized Design (CRD) with three different feed treatments, and each treatment consisted of 30 free-range female chicks as replicants, from 0-4 weeks old. Super native chick feed was formulated by trial and error by mixing all ingredients: corn, bran, soybean pods, Indigofera zollingeriana, and premix. After mixing, a proximate test determined the nutritional content of the feed. Feed was then administered to chicks. Quantity of feed was administered according to age. The results obtained in the study were analyzed descriptively and statistically using the Variance Test (ANOVA) for the variables of body weight gain, feed consumption, and FCR. The Kruskal Wallis test was used via SPSS 26 program for mortality and morbidity variables with three treatments (T1, T2, and T3) and three replications. Each treatment consisted of 30 research individuals. Results showed that the super native chicks on varied protein diets had a significant difference in performance (P0.05), with feed 3 performing the best feed 3 produced a feed consumption of 430.20 g/chick and body weight gain of 174.67 g/chick, along with 2.46 FCR, 0% mortality, and 3.33% morbidity. The conclusion this study is holistic feed made from local ingredients with the addition of Indigofera zolliengeriana had a significant effect on increasing average daily gain, increasing feed consumption, and reducing feed conversion ratio (FCR) in native super chick.
THE PRODUCTION OF FREEZE-DRIED EGG YOLK POWDER AND ITS EFFECT ON THE QUALITY OF GARUT RAM LIQUID SEMEN
Authors: Ariantie Oriza Savitri , Amrozi Amrozi , Yusuf Tuty Laswardi , Rochman Nurul Taufiqu , Purwantara Bambang 
Published: July 2021

This research aims to evaluate the protective potency of freeze-dried egg yolk powder on Garut ram sperm during the liquid semen preservation process. Semen with good sperm quality was divided into three groups and diluted using the following Tris diluents: fresh egg yolk-Tris (FEY-Tris), commercial egg yolk powder-Tris (CEY-Tris), and freeze-dried egg yolk powder-Tris (DEY-Tris). Semen that had been diluted was observed every 12 hours until sperm progressive motility was 50%. Sperm quality was tested for progressive motility percentages, viability, intact plasma membrane (IPM), and intact acrosome (IA). Fresh egg yolk had a particle size of 14460.00±330.76 nm, with polydispersity index value (PI) and zeta potential (ZP) being 1.00±0.11 and -9.22±0.30 mV, respectively. The particle size of commercial egg yolk powder was 877.90±168.86 nm with PI value 0.34±0.04 and ZP-28.7±1.24 mV. The particle size of freeze-dried egg yolk powder was 1296.00±86.73 nm, with PI value 0.70±0.04 and ZP -34.5±0.64 mV. Progressive motility percentage showed that DEY-Tris diluent managed to survive to 51.11±6.06% for 168 hours, compared to FEY-Tris (51.25±6.74%) which survived for 156 hours, although no longer than CEY-Tris diluent (53.47±6.33%) which survive for 180 hours (P0.05). Higher sperm motility was also supported by viability percentage, IPM, and IA, which were all higher (P0.05). In conclusion, freeze dried egg yolk powder mixed with Tris buffer was able to maintain sperm quality during the liquid semen preservation process.
Treatment of Osteoarthritis with Autologous, micro fragmented Adipose Tissue: A study protocol for a randomized controlled trial
Authors: Mikkelsen Rasmus Kramer , Blønd Lars , Hölmich Lisbeth Rosenkrantz , Mølgaard Cecilie , Troelsen Anders , Hölmich Per , Barfod Kristoffer Weisskirchner 
Published: July 2021

Abstract
Background: Osteoarthritis is a destructive joint disease that leads to degeneration of cartilage and other morphological changes in the joint. No medical treatment currently exists that can reverse these morphological changes. Intra-articular injection with autologous, micro fragmented adipose tissue has been suggested to relieve symptoms.Methods/Design: The study is a blinded randomized controlled trial with patients allocated in a 1:1 ratio to 2 parallel groups. Patients suffering from pain and functional impairment due to osteoarthritis Kellgren-Lawrence grade 2 – 3 in the tibiofemoral joint are eligible for inclusion. The intervention group is treated with an intra-articular injection with autologous, micro fragmented adipose tissue prepared using the Lipogems® system. The control group receives an intra-articular injection with isotonic saline. In total 120 patients are to be included. The primary outcome is The Knee injury and Osteoarthritis Outcome Score (KOOS4) evaluated at 6 months. Secondary outcomes are KOOS at 3, 12 and 24 months, the Tegner activity score, treatment failure and work status of the patient. The analysis will be conducted both as intention-to-treat and per-protocol analysis. Discussion: This trial is the first to investigate the efficacy of autologous, micro fragmented adipose tissue in a randomized controlled trial. The study uses the patient reported outcome measure Knee Injury and Osteoarthritis Outcome Score (KOOS4) after 6 months as the primary outcome, as it is believed to be a valid measure to assess the patient’s opinion about their knee and associated problems when suffering from osteoarthritis.
Physical Protection in Aggregates and Organo-Mineral Associations Contribute to Carbon Stabilization at the Transition Zone of Seasonally Saturated Wetlands
Authors: Kottkamp Anna , Jones C Nathan , Palmer Margaret A. , Tully Katherine L. 
Published: July 2021

Abstract
Wetlands store significant soil organic carbon (SOC) globally due to anoxic conditions that suppress SOC loss, yet this SOC is sensitive to climate and land use change. Seasonally saturated wetlands experience fluctuating hydrologic conditions that may also promote mechanisms known to control SOC stabilization in upland soils; these wetlands are therefore likely to be important for SOC storage at the landscape-scale. We investigated the role of physicochemical mechanisms of SOC stabilization in five seasonally saturated wetlands to test the hypothesis that these mechanisms are present, particularly in the transition between wetland and upland where soil saturation is most variable. At each wetland, we monitored water level and collected soil samples at five points along a transect from frequently saturated basin edge to rarely saturated upland. We quantified physical protection of SOC in aggregates and organo-mineral associations in mineral horizons to 0.5 m depth. As expected, SOC decreased from basin edge to upland. In the basin edge and transition zone, the majority of SOC was physically protected in macroaggregates. By contrast, overall organo-mineral associations were low, with the highest Fe concentrations (5 mg Fe g -1 soil) in the transition zone. While both stabilization mechanisms were present in the transition zone, physical protection is more likely to influence SOC stabilization during dry periods in seasonally saturated wetlands. As future climate scenarios predict changes in wetland wet and dry cycles, understanding the mechanisms by which SOC is stabilized in wetland soils is critical for predicting the vulnerability of SOC to future change.
Impact of Lockdown During COVID-19 Outbreak on Ophthalmological Emergency in a Reference Center in France
Authors: MAALEJ Rim , HAGE Rabih , Salviat Flore , VIGNAL Catherine 
Published: July 2021

Abstract
PurposeIn March 2020, the sudden rise in number of SARS-CoV2 infections in France let the government to impose a strict lockdown during which all non-urgent medical consultations were postponed. From March 17 to May 10, 2020, private medical practices were closed and telemedicine was encouraged. The consequences on ophthalmic care were dramatic with over 90% of scheduled consultations canceled. The aim of this study was to report the consultation during the 2-month strict lockdown in Paris and to analyze its impact on the visual outcomes of patients consulting in the ophthalmology emergency department (OED) Methods :Data of patients who presented to the OED of the A. de Rothschild Foundation Hospital (RFH), a tertiary ophthalmology center in Paris, France, during the lockdown period and its immediate aftermath were analyzed. The results were compared to the same time periods in the years of 2018 and 2019. Four time periods were defined and numbered chronologically: March 17 to May 10, 2018 (Period 1) ; March 17 to May 10, 2019 (Period 2); March 17 to May 10, 2020 (Period 3, the lockdown period); May 11 to June 9, 2020 (Period 4, the post-lockdown period)Results :The number of consultation was reduced by more than 50% during the lockdown period (n=2909 patients) and by 30% during the post-lockdown period (n=2622) when compared to periods 1 (n=7125) and 2 (n=8058) Even though LP4 saw an increase in the number of consulting patients, there was no raise in the rate of severe diseases (12.8% during LP3 vs 11.1% during LP4) and the proportion of patients who were padmitted was statistically similar (4.3% VS 3.6%). Neuro-ophthalmic -diseases were the most common during LP3 and LP4. Neovascular glaucoma were twice as common during post LP4 (p=0.08). We highlighted a significant increase in patients with a graft reject consulting in our OED during the post LP4 (p<0.001). Those results were likely related to a delay in the follow-up consultations due to the lockdown measures.ConclusionThe reduction in the number of consultations in our OED during the lockdown period did not focus mainly on minor emergencies and also affected severe ophthalmic diseases with no significant delay in consultation. More longitudinal and longer study is needed to confirm this and to analyze retrospectively the COVID-19 outbreak and lockdown effects.
The Effects of Theory-Based Intervention on Self-Care Ability and Complications in Female Patients with Cancer with Peripherally Inserted Central Catheters (PICCs)
Authors: Huang Rong , Wu Na , Wang Ye , Jiang Hui , Zhang Li , Wang Hao 
Published: July 2021

Abstract
Purpose To examine the effects of Orem’s self-care theory-based continuous nursing intervention on self-care ability and PICC related complications and adverse events rates of female patients with cancer in the intermission of chemotherapy.Methods A quasi-experimental study was adopted. Totally 130 female patients with cancer were recruited from October 2017 to March 2019 at a tertiary maternal hospital in Shanghai. They were randomly divided into the experimental group (n=65) and the control group (n=65). The experimental group accepted nursing intervention based on Orem’s self-care theory, which had five sessions, while the control group accepted routine intervention. The exercise of self-care agency (ESCA) and the rates of PICC related complications and adverse events were compared by Chi square test and two‐sample t test between two groups through IBM SPSS 20.0 statistics software.Results The results showed that before the intervention, the baseline of two groups did not differ significantly. After the intervention, four dimensions and total scores of ESCA in the experimental group were significantly improved compared with those in the control group (p < 0.05), and the rates of PICC related complications and adverse events were significantly decreased in the experimental group compared with those in the control group (p < 0.05).Conclusions Orem’s self-care theory-based continuous nursing intervention was effective in improving self-care ability and decreasing the rates of PICC related complications and adverse events. Consequently, using the intervention program for patients with PICC during the intermission of chemotherapy is recommended.
The Impact of A Procedural Nursing Intervention to Prevent Deep Vein Thrombosis in Patients after Laparoscopic Distal Pancreatectomy and Splenectomy: A Retrospective Cohort Study
Authors: Xu Shan , Xiao Li , Wang Min , Zeng Tieying 
Published: July 2021

Abstract
Purpose The purpose of this study was to investigate the effect of procedural nursing intervention to prevent deep vein thrombosis (DVT) in patients after laparoscopic distal pancreatectomy and splenectomy (LDPS).Methods We conducted a retrospective analysis of data from 80 patients with tumours of the pancreatic corpus or tail who underwent surgery at a high volume single centre. T-test and c2 test were performed for data analysis. Study variables included incidence of DVT after LDPS, coagulation function indexes, and patient satisfaction. Results For patients after LDPS, individuals receiving the procedural nursing intervention had a lower incidence of DVT compared to individuals receiving routine nursing care. There was no significant difference in Fibrinogen (FBI), D-Dimer(D-D), Activated Partial Thromboplastin Time (APTT), Prothrombin Time (PT), Thrombin Time (TT) between the two groups (P>0.05). Platelet Count (PLT) among individuals receiving the procedural nursing intervention were significantly lower than those receiving routine nursing care. The procedural nursing interventions were associated with higher patient satisfaction compared to routine care. Conclusions A procedural nursing intervention contributes to a lower incidence of DVT, lower PLT, higher satisfaction.
REMBRANDT: A High-Throughput Barcoded Sequencing Approach for COVID-19 Screening.
Authors: Palmieri Dario , Javorina Amanda , Siddiquii Jalal , Gardner Anne , Fries Anthony , Chapleau Richard , Starr Clarise , Fishel Richard , Miles wayne 
Published: July 2021

Abstract
The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), also known as 2019 novel coronavirus (2019-nCoV), is a highly infectious RNA virus. A percentage of patients develop coronavirus disease 2019 (COVID-19) after infection, whose symptoms include fever, cough, shortness of breath and fatigue. Acute and life-threatening respiratory symptoms are experienced by 10-20% of symptomatic patients, particularly those with underlying medical conditions. One of the main challenges in the containment of COVID-19 is the identification and isolation of asymptomatic/pre-symptomatic individuals. A number of molecular assays are currently used to detect SARS-CoV-2. Many of them can accurately test hundreds or even thousands of patients every day. However, there are presently no testing platforms that enable more than 10,000 tests per day. Here, we describe the foundation for the REcombinase Mediated BaRcoding and AmplificatioN Diagnostic Tool (REMBRANDT), a high-throughput Next Generation Sequencing-based approach for the simultaneous screening of over 100,000 samples per day. The REMBRANDT protocol includes direct two-barcoded amplification of SARS-CoV-2 and control amplicons using an isothermal reaction, and the downstream library preparation for Illumina sequencing and bioinformatics analysis. This protocol represents a potentially powerful approach for community screening of COVID 19 that may be modified for application to any infectious or non-infectious genome.
Integrative Analysis and Identification of an Excellent lncRNA Signature to Predict Prognosis in Patients with COAD
Authors: Chen ZhiHua , Lin YiLin , Lin SuYong , Gao Ji , Chen Shao-Qin 
Published: July 2021

Abstract
Backgroud: Tumour recurrence and metastasis lead to poor prognosis incolon cancer(COAD). Therefore We aimed to identify a lncRNA signature through an integrative analysis of copy number variation, mutation and transcriptome data to predict prognosis and explore its internal mechanism.Methods: The lncRNA expression profile were collected fromThe Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). TCGA data was randomly divided 3:1 intotraining andtesting cohort. In the training, weperformed integrated analyses of three candidate lncRNA sets that correlated with prognosis, copy number variations and mutations to establish a signature through Cox regression analysis. The robustness was determined in the testing and GEO.Results: An 11-lncRNA signature that was significantly associated with prognosiswas constructed in the training (P<0.0001, HR=2.014) , And this signature was validated in the testing(P=0.0019, HR=3.374) and GSE17536(P=0.0076, HR=1.864). The signature is significantly related to MSI status and clinical prognostic factors. The prognostic-relatedrisk scores were significantly excellent than the other five models have been reported. Furthermore, GSEA suggested that the signature was involved in COAD development and metastasis-related pathways.Conclusions: We identifiedansignature has strong robustness and can stably predict the prognosis of COAD in different platformsand may be implicated in COAD pathogenesis and metastasis and applied clinically as a prognostic marker.
In Vivo Quantitative Assessment of Therapeutic Response to Bortezomib Therapy in Disseminated Animal Models of Multiple Myeloma With [18F]FDG and [64Cu]LLP2A PET
Authors: Ghai Anchal , Fettig Nikki , Fontana Francesca , DiPersio John , Rettig Mike , Neal Julie O , Achilefu Samuel , Shoghi Kooresh , Shokeen Monica 
Published: July 2021

Abstract
BackgroundMultiple myeloma (MM) is a disease of cancerous plasma cells. Current treatments have improved the survival rate; however, most MM patients relapse. Imaging based timely determination of therapeutic response is critical for improving outcomes in MM patients. Very late antigen-4 (VLA4) is over expressed in MM cells. Here, we evaluated [18F]FDG and VLA4 targeted [64Cu]LLP2A for quantitative PET imaging in MM models of variable VLA4 expression, and following bortezomib therapy.MethodsIn vitro and ex vivo VLA4 expression was evaluated by flow cytometry. Human MM cells, MM.1S-CG and U266-CG (CG: luciferase and green fluorescent protein), were injected intravenously in NOD-SCID gamma mice. Tumor progression was monitored by bioluminescence imaging (BLI). Treatment group received bortezomib (1mg/kg, twice/week) intraperitoneally. All cohorts (treated, untreated and no-tumor) were longitudinally imaged with [64Cu]LLP2A (2-3 MBq; Molar Activity: 44.14±1.40 MBq/nmol) and [18F]FDG (7.4-8.0 MBq) PET respectively.ResultsFlow cytometry confirmed high expression of CD49d in U266 cells (>99%) and moderate expression in MM.1S cells (~52%). BLI showed decrease in total body flux in treated mice. In MM.1S-CG untreated versus treated mice, [64Cu]LLP2A localized with a significantly higher SUVmean in spine (0.58 versus 0.31) and femur (0.72 versus 0.39) at week 4 post tumor inoculation. In U266-CG treated versus untreated mice, there was a 4-time percent [64Cu]LLP2A increase in spine at week 3. Compared to [64Cu]LLP2A, [18F]FDG PET detected treatment related changes at later time points.Conclusion[64Cu]LLP2A is a promising tracer for in vivo assessment of therapeutic response in disseminated models of MM.
Skp2 Inhibitor AAA-237 Exerts Anti-Tumor Effect on NSCLC by Inducing Mitochondria-Dependent Apoptosis and Senescence
Authors: Liu Jinyi , Zheng Xiangjin , Li Wan , Ren Liwen , Li Sha , Yang Yihui , Ge Binbin , Du Guanhua , Shi Jianyou , Wang Jinhua 
Published: July 2021

Abstract
Background: Lung cancer is by far the leading cause of cancer death worldwide, and 85% of patients are non-small cell lung cancer (NSCLC). NSCLC is still very difficult to treat. Skp2 functions as an oncogene and participates in the processes of NSCLC. Here, we disclose a novel Skp2 inhibitor, which is firstly synthesized by our group based on our previous computer virtual screening and biomolecular interaction analysis (Biacore) technology screening. Methods: To unveil antitumor activity and underlying molecular mechanism of AAA-237, cell proliferation, migration and invasion assays, apoptosis, cell cycle, senescence, and xenograft mouse models were used.Results: AAA-237 inhibited the proliferation of NSCLC cells. AAA-237 regulated the process of epithelial–mesenchymal transition (EMT) by Skp2/Twist axis. Furthermore, AAA-237 induced arresting of cell cycle at G0/G1 phase by regulating the Skp2-Cip/Kip and PI3K/Akt-FOXO1 signaling pathways. More importantly, we found that treatment of high concentration AAA-237 could induce apoptosis of NSCLC cells via the mitochondrial pathway whereas treatment of low concentration AAA-237 for long-term could induce senescence of NSCLC cells by DNA damage. Senolytic drug quercetin could selectively eliminate senescent cells induced by AAA-237. In A549 xenograft nude mice model, AAA-237 dose-dependently inhibited the growth of tumor by inducing apoptosis and senescence.Conclusions: Taken together, we propose that AAA-237 is a promising therapeutic agent of NSCLC, especially when combined with senolytic drug.
Co-Delivery of Doxorubicin and Conferone by Novel pH-Responsive β-Cyclodextrin Grafted Micelles Triggers Apoptosis of Metastatic Human Breast Cancer Cells
Authors: Rahmani Akram , Rahimi Fariborz , Iranshahi Mehrdad , Kahroba Houman , Zarebkohan Amir , Talebi Mehdi , Salehi Roya , Mousavi Hassan Zavvar 
Published: July 2021

Abstract
Adjuvant-aided combination chemotherapy is one of the most effective ways of cancer treatment by overcoming the multidrug resistance (MDR) and reducing the side-effects of anticancer drugs. In this study Conferone (Conf) was used as adjuvant in combination with Doxorubicin (Dox) for induction of apoptosis to MDA-MB-231 cells. Herein, the novel biodegradable amphiphilic β-cyclodextrin grafted poly maleate-co-PLGA was synthesized by thiol-ene addition and ring-opening process. Micelles obtained from novel copolymer showed exceptional properties such as small size of around 34.5 nm, CMC of 0.1 μg/mL, and cell internalization of around 100 % at 30 min. These novel engineered micelles were used for combination delivery of doxorubicin-conferone with high encapsulation efficiency of near 100 % for both drugs. Our results show that combination delivery of Dox and Conf to MDA-MB-231 cells had synergistic effects (CI<1). According to cell cycle and Annexin-V apoptosis analysis, Dox-Conf loaded micelle significantly induce tumor cell apoptosis (more than 98 % of cells population showed apoptosis at IC50 = 0.259 μg/mL). RT-PCR and western-blot tests show that Dox-Conf loaded βCD-g-PMA-co-PLGA micelle induced apoptosis via intrinsic pathway. Therefore, the unique design of multi-functional pH-sensitive micelles open a new perspective for the development of nanomedicines for combination chemo-adjuvant therapy against malignant cancer.