bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adipose-specific VDR Deletion Leads to Hepatic Steatosis in Female Mice Fed a Low-Fat Diet
Authors: Tao Tao , Kobelski Margaret M , Saini Vaibhav , Demay Marie B 
Published: December 2021

AbstractRisk factors for nonalcoholic hepatic steatosis include obesity and vitamin D deficiency which commonly coexist. Thus, the role of vitamin D signaling in the prevention of hepatic steatosis in the absence of obesity or a “Western” high-fat diet is unclear. These studies were performed to address the role of the adipocyte vitamin D receptor (VDR) in the prevention of hepatic steatosis in mice fed a chow diet containing 5% fat by weight.Female mice with adipocyte VDR ablation (Adipoq-Cre; VDRflox/flox) exhibited a mild increase in weight gain at age 70 days, accompanied by an increase in visceral white adipose tissue (VAT) weight. While they did not exhibit evidence of hepatic inflammation or fibrosis, an increase in hepatic lipid content was observed. This was accompanied by an increase in the hepatic expression of genes involved in fatty acid transport and synthesis, as well as fatty acid oxidation. Markers of hepatic inflammation and fibrosis were unaffected by adipocyte VDR ablation. Consistent with the increase in VAT weight in the Adipoq-Cre; VDRflox/flox mice, higher levels of transcripts encoding adipogenesis-related genes were observed in VAT.In contrast to other models of impaired vitamin D signaling studied in the setting of a high-fat or “Western” diet, the Adipoq-Cre; VDRflox/flox mice do not exhibit hepatic inflammation or fibrosis. These findings suggest that the adipocyte VDR regulates hepatic lipid accumulation, but in the absence of obesity or a high-fat diet, is not required to prevent hepatic inflammation or fibrosis.
Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage
Authors: Colombo Sophie , Petit Valérie , Wagner Roselyne Y. , Champeval Delphine , Yajima Ichiro , Gesbert Franck , Aktary Zackie , Davidson Irwin , Delmas Véronique , Larue Lionel 
Published: December 2021

ABSTRACTThe canonical Wnt/β-catenin pathway governs a multitude of developmental processes in various cell lineages, including the melanocyte lineage. Indeed, β-catenin regulates transcription of Mitf-M, the master regulator of this lineage. The first wave of melanocytes to colonize the skin is directly derived from neural crest cells, whereas the second wave of melanocytes is derived from Schwann cell precursors (SCPs). We investigated the influence of β-catenin in the development of melanocytes of the first and second waves by generating mice expressing a constitutively active form of β-catenin in cells expressing tyrosinase. Constitutive activation of β-catenin did not affect the development of truncal melanoblasts but led to marked hyperpigmentation of the paws. By activating β-catenin at various stages of development (E8.5-E11.5), we showed that the activation of β-catenin in bipotent SCPs favored melanoblast specification at the expense of Schwann cells in the limbs within a specific temporal window. Furthermore, in vitro hyperactivation of the Wnt/β-catenin pathway, which is required for melanocyte development, induces activation of Mitf-M, in turn repressing FoxD3 expression. In conclusion, β-catenin overexpression promotes SCP cell fate decisions towards the melanocyte lineage.
Msl3 promotes germline stem cell differentiation in female Drosophila
Authors: McCarthy Alicia , Sarkar Kahini , Martin Elliot T. , Upadhyay Maitreyi , Jang Seoyeon , Williams Nathan D. , Forni Paolo E. , Buszczak Michael , Rangan Prashanth 
Published: December 2021

ABSTRACT
Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation is incompletely understood. Set2, which deposits H3K36me3 modifications, is required for GSC differentiation during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (Msl3) and histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate GSC differentiation in female Drosophila. Msl3, acting independently of the rest of the male-specific lethal complex, promotes transcription of genes, including a germline-enriched ribosomal protein S19 paralog RpS19b. RpS19b upregulation is required for translation of RNA-binding Fox protein 1 (Rbfox1), a known meiotic cell cycle entry factor. Thus, Msl3 regulates GSC differentiation by modulating translation of a key factor that promotes transition to an oocyte fate.
Visualizing polymeric components that define distinct root barriers across plant lineages
Authors: Sexauer Moritz , Shen Defeng , Schön Maria , Andersen Tonni Grube , Markmann Katharina 
Published: December 2021

ABSTRACT
Hydrophobic cell wall depositions in roots play a key role in plant development and interaction with the soil environment, as they generate barriers that regulate bidirectional nutrient flux. Techniques to label the respective polymers are emerging, but are efficient only in thin roots or sections. Moreover, simultaneous imaging of the barrier constituents lignin and suberin remains problematic owing to their similar chemical compositions. Here, we describe a staining method compatible with single- and multiphoton confocal microscopy that allows for concurrent visualization of primary cell walls and distinct secondary depositions in one workflow. This protocol permits efficient separation of suberin- and lignin-specific signals with high resolution, enabling precise dissection of barrier constituents. Our approach is compatible with imaging of fluorescent proteins, and can thus complement genetic markers or aid the dissection of barriers in biotic root interactions. We further demonstrate applicability in deep root tissues of plant models and crops across phylogenetic lineages. Our optimized toolset will significantly advance our understanding of root barrier dynamics and function, and of their role in plant interactions with the rhizospheric environment.
Urinoir Analyzer Pintar Pendeteksi Kelainan Pada Fungsi Ginjal Dengan Analisis Kadar Ph Dan Warna Pada Urin
Authors: Febryansah M. Iqbal , Yudhana Anton , Ma'arif Alfian 
Published: December 2021

Perkembangan pemeriksaan penyakit kelainan ginjal melalui analisa urin saat ini dilakukan dalam dua proses pemeriksaan secara makroskopis dan mikroskopis. Pada dasarnya dibutuhkan sebuah alat yang mampu memproses dan menganalisis sebuah sampel urin secara otomatis agar tidak terjadinya kesalahan dalam melakukan pemeriksaan penyakit melalui sampel urin. Awalan perkembangan ini menggunakan sebuah Kontroler Arduino UNO dan dua buah variabel masukan yaitu sensor warna TCS3200 dan sensor PH meter SKU SEN0161. Dua buah variabel masukan sensor bekerja secara berdampingan dengan sensor TCS3200 memiliki hasil keluaran berupa nilai frekuensi RGB dan diproses kembali menjadi frekuensi keabuan. Lalu, sensor PH meter SKU SEN0161 menghasilkan sebuah nilai PH pada sampel urin. Hasil dari pemeriksaan tersebut ditampilkan pada sebuah penampil berupa LCD berukuran 16x2. Hasil pemeriksaan dari alat ini dibandingkan dengan hasil analisa pakar dari Balai Laboratorium Kesehatan Yogyakarta bagian Urology. dan mendapatkan tingkatan nilai akurasi 93% dengan keberhasilan data sebanyak 28 dari 30 data yang diambil.Â The development of examining kidney disorders through urine analysis is currently carried out in two processes of examination, macroscopic and microscopic. Basically, we need a tool that is able to process and analyze a urine sample automatically so that there are no errors in carrying out disease checks through the urine sample. The beginning of this development used an Arduino UNO controller and two input variables, namely the TCS3200 color sensor and the SKU SEN0161 PH meter sensor. Two sensor input variables working side by side with the TCS3200 sensor have an output in the form of RGB frequency values and are processed back into gray frequencies. Then, the PH meter SKU SEN0161 sensor generates a PH value in the urine sample. The results of these checks are displayed on a 16x2 LCD display. The examination results of this tool are compared with the results of the analysis of experts from the Yogyakarta Health Laboratory Center, Urology section. and get an accuracy level of 93% with the success of the data as much as 28 of the 30 data taken.
Single Exponential Smoothing-Multilayer Perceptron Untuk Peramalan Pengunjung Unik Jurnal Elektronik
Authors: Ferdinand Miftakhul Anggita Bima , Wibawa Aji Prasetya , Zaeni Ilham Ari Elbaith , Rosyid Harits Ar 
Published: December 2021

Jumlah kunjungan rerata pengunjung unik per hari pada jurnal elektronik menunjukkan bahwa hasil terbitan karya ilmiah website tersebut menarik. Sehingga jumlah pengunjung unik dijadikan indikator penting dalam mengukur keberhasilan sebuah jurnal elektronik untuk memenuhi perluasan, penyebaran dan percepatan sistem akreditasi jurnal. Pengunjung Unik merupakan jumlah pengunjung per Internet Address (IP) yang mengakses sebuah jurnal elektronik dalam kurun waktu tertentu. Terdapat beberapa metode yang biasa digunakan untuk peramalan, diantaranya adalah Multilayer Perceptron (MLP).Â Â Kualitas data berpengaruh besar dalam membangun model MLP yang baik, karena sukses tidaknya permodelan pada MLP sangat dipengaruhi oleh data input. Salah satu cara untuk meningkatkan kualitas data adalah dengan melakukan smoothing pada data tersebut. Pada penelitian ini digunkan metode peramalan Multilayer Perceptron berdasarkan penelitian sebelumnya dengan kombinasi data training dan testing 80%-20% dengan asitektur 2-1-1 dan learning rate 0,4. Selanjutnya untuk meningkatkan kualitas data dilakukan smoothing dengan menerapkan metode Single Exponential Smoothing. Dari penelitian yang dilakukan diperoleh hasil terbaik menggunakan alpha 0.9 dengan hasil akurasi MSE 94.02% dan RMSE 75.54% dengan lama waktu eksekusi 580,27 detik.Â The number of visits by the average unique visitor per day on electronic journals shows that the published scientific papers on the website are interesting. So that the number of unique visitors is used as an important indicator in measuring the success of an electronic journal to meet the expansion, dissemination and acceleration of the journal accreditation system. Unique Visitors is the number of visitors per Internet Address (IP) who access an electronic journal within a certain period of time. There are several methods commonly used for forecasting, including the Multilayer Perceptron (MLP). Data quality has a big influence in building a good MLP model, because the success or failure of modeling in MLP is greatly influenced by the input data. One way to improve data quality is by smoothing the data. In this study, the Multilayer Perceptron forecasting method was used based on previous research with a combination of training data and testing 80% -20% with a 2-1-1 architecture and a learning rate of 0.4. Furthermore, to improve data quality, smoothing is done by applying the Single Exponential Smoothing method. From the research conducted, the best results were obtained using alpha 0.9 with MSE accuracy of 94.02% and RMSE 75.54% with a long execution time of 580.27 seconds.
Cryogenic integrated spontaneous parametric down-conversion
Authors: Lange Nina Amelie , Höpker Jan Philipp , Ricken Raimund , Quiring Viktor , Eigner Christof , Silberhorn Christine , Bartley Tim J. 
Published: December 2021

Scalable quantum photonics relies on interfacing many optical components under mutually compatible operating conditions. To that end, we demonstrate that spontaneous parametric down-conversion (SPDC) in nonlinear waveguides, a standard technology for generating entangled photon pairs, squeezed states, and heralded single photons, is fully compatible with cryogenic operating conditions required for superconducting detectors. This is necessary for the proliferation of integrated quantum photonics in integration platforms exploiting quasi-phase-matched second-order nonlinear interactions. We investigate how cryogenic operation at 4 K affects the SPDC process by comparing the heralding efficiency, second-order correlation function, and spectral properties with operation at room temperature.
A MEKK1 – JNK mitogen activated kinase (MAPK) cascade module is active in Echinococcus multilocularis stem cells
Authors: Stoll Kristin , Bergmann Monika , Spiliotis Markus , Brehm Klaus 
Published: December 2021

Background
The metacestode larval stage of the fox-tapeworm Echinococcus multilocularis causes alveolar echinococcosis by tumour-like growth within the liver of the intermediate host. Metacestode growth and development is stimulated by host-derived cytokines such as insulin, fibroblast growth factor, and epidermal growth factor via activation of cognate receptor tyrosine kinases expressed by the parasite. Little is known, however, concerning signal transmission to the parasite nucleus and cross-reaction with other parasite signalling systems.

Methodology/Principal findings
Using bioinformatic approaches, cloning, and yeast two-hybrid analyses we identified a novel mitogen-activated kinase (MAPK) cascade module that consists of E. multilocularis orthologs of the tyrosine kinase receptor interactor Growth factor receptor-bound 2, EmGrb2, the MAPK kinase kinase EmMEKK1, a novel MAPK kinase, EmMKK3, and a close homolog to c-Jun N-terminal kinase (JNK), EmMPK3. Whole mount in situ hybridization analyses indicated that EmMEKK1 and EmMPK3 are both expressed in E. multilocularis germinative (stem) cells but also in differentiated or differentiating cells. Treatment with the known JNK inhibitor SP600125 led to a significantly reduced formation of metacestode vesicles from stem cells and to a specific reduction of proliferating stem cells in mature metacestode vesicles.

Conclusions/Significance
We provide evidence for the expression of a MEKK1-JNK MAPK cascade module which, in mammals, is crucially involved in stress responses, cytoskeletal rearrangements, and apoptosis, in E. multilocularis stem cells. Inhibitor studies indicate an important role of JNK signalling in E. multilocularis stem cell survival and/or maintenance. Our data are relevant for molecular and cellular studies into crosstalk signalling mechanisms that govern Echinococcus stem cell function and introduce the JNK signalling cascade as a possible target of chemotherapeutics against echinococcosis.
COVID-19: Post-recovery long-term symptoms among patients in Saudi Arabia
Authors: Khodeir Mostafa M. , Shabana Hassan A. , Rasheed Zafar , Alkhamiss Abdullah S. , Khodeir Mohamed , Alkhowailed Mohammad S. , Alharbi Sami , Alsoghair Mansour , Alsagaby Suliman A. , Al Abdulmonem Waleed 
Published: December 2021

Background
After recovery from acute infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many patients experience long-term symptoms in different body systems. The aim of the present study was to identify these symptoms, their severity, and their duration as a first step in building a system to classify post-recovery long-term symptoms of coronavirus disease 2019 (COVID-19).

Methods
An online-based cross-sectional survey was administered between September and October 2020. Data regarding the severity of post-recovery symptoms and their duration were collected using an Arabic questionnaire divided into six categories encompassing the 20 most prevalent symptoms.

Results
A total of 979 patients recovered from COVID-19 in Saudi Arabia in the study period, of whom 53% were male and 47% were female. The most common symptoms included general fatigue and weakness (73% each), with moderate severity of neurological symptoms including mood changes (41%) and insomnia (39%). Among the special senses, loss of smell and taste of marked severity were reported by 64% and 55% among respiratory symptoms, cough of mild severity (47%), and dyspnea of moderate severity (43%). Loss of appetite of moderate severity was reported in 42%, and diarrhea, abdominal pain, and nausea of mild severity were reported by 53%, 50%, and 44% of respondents, respectively.

Conclusions
Long-term symptoms after recovery from COVID-19 warrant patient follow-up. The authors propose a classification system as a starting point to guide the identification and follow-up of long-term symptoms post-recovery, and recommend larger-scale studies to broaden the definition of recovery from COVID-19, which appears to have two phases, acute and chronic.
Vaping discussion in the COVID-19 pandemic: An observational study using Twitter data
Authors: Lyu Joanne Chen , Luli Garving K. , Ling Pamela M. 
Published: December 2021

Background
With the spread of COVID-19, significant concerns have been raised about the potential increased risk for electronic cigarette (e-cigarette) users for COVID-19 infection and related syndromes. Social media is an increasingly popular source for health information dissemination and discussion, and can affect health outcomes.

Objective
This study aims to identify the topics in the public vaping discussion in COVID-19–related Twitter posts in order to get insight into public vaping-related perceptions, attitudes and concerns, and to discern possible misinformation and misconceptions around vaping in the COVID-19 pandemic.

Methods
Using the tweets ID database maintained by Georgia State University’s Panacea Lab, we downloaded the tweets related to COVID-19 from March 11, 2020, when the World Health Organization declared COVID-19 a pandemic, to February 12, 2021. We used R to analyze the tweets that contained a list of 79 keywords related to vaping. After removing duplicates and tweets created by faked accounts or bots, the final data set consisted of 11,337 unique tweets from 7,710 different users. We performed the latent Dirichlet allocation (LDA) algorithm for topic modeling and carried out a sentiment analysis.

Results
Despite fluctuations, the number of daily tweets was relatively stable (average number of daily tweets = 33.4) with a sole conspicuous spike happening on a few days after August 11, 2020 when a research team published findings that teenagers and young adults who vape face a much higher risk of COVID-19 infection than their peers who do not vape. Topic modeling generated 8 topics: linkage between vaping and risk of COVID-19 infection, vaping pneumonia and the origin of COVID-19, vaping and spread of COVID-19, vaping regulation, calling for quitting vaping, protecting youth, similarity between e-cigarette or vaping-associated lung injury (EVALI) and COVID-19, and sales information. Daily sentiment scores showed that the public sentiment was predominantly negative, but became slightly more positive over the course of the study time period.

Conclusions
While some content in the public discourse on vaping before the COVID-19 pandemic continued in Twitter posts during the COVID-19 time period, new topics emerged. We found a substantial amount of anti-vaping discussion and dominantly negative sentiment around vaping during COVID-19, a sharp contrast to the predominantly pro-vaping voice on social media in the pre-COVID-19 period. Continued monitoring of social media conversations around vaping is needed, and the public health community may consider using social media platforms to actively convey scientific information around vaping and vaping cessation.
Ranking lifestyle risk factors for cervical cancer among Black women: A case-control study from Johannesburg, South Africa
Authors: Singini Mwiza Gideon , Sitas Freddy , Bradshaw Debbie , Chen Wenlong Carl , Motlhale Melitah , Kamiza Abram Bunya , de Villiers Chantal Babb , Lewis Cathryn M. , Mathew Christopher G. , Waterboer Tim , Newton Robert , Muchengeti Mazvita , Singh Elvira 
Published: December 2021

Background
Aside from human papillomavirus (HPV), the role of other risk factors in cervical cancer such as age, education, parity, sexual partners, smoking and human immunodeficiency virus (HIV) have been described but never ranked in order of priority. We evaluated the contribution of several known lifestyle co-risk factors for cervical cancer among black South African women.

Methods
We used participant data from the Johannesburg Cancer Study, a case-control study of women recruited mainly at Charlotte Maxeke Johannesburg Academic Hospital between 1995 and 2016. A total of 3,450 women in the study had invasive cervical cancers, 95% of which were squamous cell carcinoma. Controls were 5,709 women with cancers unrelated to exposures of interest. Unconditional logistic regression models were used to calculate adjusted odds ratios (ORadj) and 95% confidence intervals (CI). We ranked these risk factors by their population attributable fractions (PAF), which take the local prevalence of exposure among the cases and risk into account.

Results
Cervical cancer in decreasing order of priority was associated with (1) being HIV positive (ORadj = 2.83, 95% CI = 2.53–3.14, PAF = 17.6%), (2) lower educational attainment (ORadj = 1.60, 95% CI = 1.44–1.77, PAF = 16.2%), (3) higher parity (3+ children vs 2–1 children (ORadj = 1.25, 95% CI = 1.07–1.46, PAF = 12.6%), (4) hormonal contraceptive use (ORadj = 1.48, 95% CI = 1.24–1.77, PAF = 8.9%), (5) heavy alcohol consumption (ORadj = 1.44, 95% CI = 1.15–1.81, PAF = 5.6%), (6) current smoking (ORadj = 1.64, 95% CI = 1.41–1.91, PAF = 5.1%), and (7) rural residence (ORadj = 1.60, 95% CI = 1.44–1.77, PAF = 4.4%).

Conclunsion
This rank order of risks could be used to target educational messaging and appropriate interventions for cervical cancer prevention in South African women.
Case management programs for people with complex needs: Towards better engagement of community pharmacies and community-based organisations
Authors: Chouinard Maud-Christine , Bisson Mathieu , Danish Alya , Karam Marlène , Beaudin Jérémie , Grgurevic Nevena , Sabourin Véronique , Hudon Catherine 
Published: December 2021

Introduction
The objectives of this study were 1) to describe how case management programs engaged community pharmacies and community-based organisations in a perspective of integrated care for people with complex needs, and 2) to identify enablers, barriers and potential strategies for this engagement.

Methods
Using a descriptive qualitative design, individual interviews and focus groups with patients, healthcare providers and managers were analysed according to a mixed thematic analysis based on a deductive (Rainbow Model of Integrated Care) and an inductive approach.

Results and discussion
Participants highlighted the individualized service plan as a significant tool to foster a shared person-focused vision of care, information exchanges and concerted efforts. Openness to collaboration was also considered as an enabler for community stakeholders’ engagement. The lack of recognition of community-based organisations by certain providers and the time required to participate in individualized service plans were outlined as barriers to professional integration. Limited opportunities for community stakeholders to be involved in decision-making within case management programs were reported as another constraint to their engagement. Cultural differences between organisations regarding the focus of the intervention (psychosocial vs healthcare needs) and differences in bureaucratic structures and funding mechanisms may negatively affect community stakeholders’ engagement. Formal consultation mechanisms and improvement of communication channels between healthcare providers and community stakeholders were suggested as ways to overcome these barriers.

Conclusion
Efforts to improve care integration in case management programs should be directed toward the recognition of community stakeholders as co-producers of care and co-builders of social policies across the entire care continuum for people with complex needs.