bluuebunny/crossref_metadata_2025_split huggingface dataset:
SuperPath approach is a recommendable option in frail patients with femoral neck fractures: a case–control study
Authors: Cecere Antonio Benedetto , De Cicco Annalisa , Bruno Gaetano , Toro Giuseppe , Errico Giacomo , Braile Adriano , Schiavone Panni Alfredo 
Published: September 2021

Abstract
Introduction
The treatment of intracapsular femoral neck fractures (FNFs) in the elderly is usually based on hip replacement, both total hip arthroplasty (THA) and hemiarthroplasty (HA). Recently, several tissue-sparing approaches for hip arthroplasty had been described with promising results in terms of hospitalization length, blood loss and dislocation rate. The aim of the present study was to compare the blood loss and the transfusion rate in a cohort of patients with FNF treated using an HA through both the SuperPath (SP) and the traditional posterolateral (PL) approaches.

Materials and methods
We retrospectively collected data from patients affected by FNFs between January 2018 and February 2020. All patients with intracapsular FNF treated with a single HA implant (Profemur L, MicroPort Orthopedics Inc., USA) via PL or SP approaches were included. Exclusion criteria were pathological fractures, polytrauma and preoperatively transfused patients.

Results
Thirty-five patients were included and analysed in the present study. 17 patients were classified in the SP group, and 18 in the PL one. The rate of antithrombotic therapy was higher in the SP group compared with the PL group [10 (58, 82%) vs 4 (22, 2%)]. While the two groups did not differ in terms of preoperative haemoglobin (Hb), 48 h postoperative Hb and Hb reduction, a significative difference was observed in terms of blood transfusion rate (1 SP vs 9 PL, p = 0.0072).

Conclusions
The SuperPath approach in patients with FNF under antithrombotic therapy assures lower transfusion rate, potentially reducing complication rates and improving patients' outcomes.
A retrospective study on long-term efficacy of intranasal lysine-aspirin in controlling NSAID-exacerbated respiratory disease
Authors: Pendolino Alfonso Luca , Scadding Glenis K. , Scarpa Bruno , Andrews Peter J. 
Published: September 2021

Abstract
Purpose
Aspirin treatment after desensitization (ATAD) represents an effective therapeutic option suitable for NSAID-exacerbated respiratory disease (N-ERD) patients with recalcitrant disease. Intranasal administration of lysine-aspirin (LAS) has been suggested as a safer and faster route than oral ATAD but evidence for its use is less strong. We investigated nasal LAS therapy long-term efficacy based on objective outcomes, smell function, polyp recurrence and need for surgery or rescue therapy. Clinical biomarkers predicting response to intranasal LAS, long-term side effects and consequences of discontinuing treatment have been evaluated.

Methods
A retrospective analysis of a database of 60 N-ERD patients seen between 2012 and 2020 was performed in March 2021. They were followed up at 3-months, 1-, 2- and 3-years with upper and lower airway functions assessed at each follow-up.

Results
Higher nasal airflow and smell scores were found at each follow-up in patients taking LAS (p < 0.001 and p = 0.048 respectively). No influence of LAS on pulmonary function measurements was observed. Patient on intranasal LAS showed a lower rate of revision sinus surgery when compared to those who discontinued the treatment (p < 0.001). None of the variables studied was found to influence LAS treatment response.

Conclusion
Our study demonstrates the clinical effectiveness of long-term intranasal LAS in the management of N-ERD in terms of improved nasal airflow and olfaction and a reduced need for revision sinus surgery. Intranasal LAS is safe, being associated with a lower rate of side effects when compared to oral ATAD. However, discontinuation of the treatment at any stage is associated with a loss of clinical benefit.
Interobserver variation in the classification of tumor deposits in rectal cancer—is the use of histopathological characteristics the way to go?
Authors: Brouwer Nelleke P. M. , Lord A. C. , Terlizzo M. , Bateman A. C. , West N. P. , Goldin R. , Martinez A. , Wong N. A. C. S. , Novelli M. , Nagtegaal I. D. , Brown G. 
Published: September 2021

Abstract
The focus on lymph node metastases (LNM) as the most important prognostic marker in colorectal cancer (CRC) has been challenged by the finding that other types of locoregional spread, including tumor deposits (TDs), extramural venous invasion (EMVI), and perineural invasion (PNI), also have significant impact. However, there are concerns about interobserver variation when differentiating between these features. Therefore, this study analyzed interobserver agreement between pathologists when assessing routine tumor nodules based on TNM 8. Electronic slides of 50 tumor nodules that were not treated with neoadjuvant therapy were reviewed by 8 gastrointestinal pathologists. They were asked to classify each nodule as TD, LNM, EMVI, or PNI, and to list which histological discriminatory features were present. There was overall agreement of 73.5% (κ 0.38, 95%-CI 0.33–0.43) if a nodal versus non-nodal classification was used, and 52.2% (κ 0.27, 95%-CI 0.23–0.31) if EMVI and PNI were classified separately. The interobserver agreement varied significantly between discriminatory features from κ 0.64 (95%-CI 0.58–0.70) for roundness to κ 0.26 (95%-CI 0.12–0.41) for a lone arteriole sign, and the presence of discriminatory features did not always correlate with the final classification. Since extranodal pathways of spread are prognostically relevant, classification of tumor nodules is important. There is currently no evidence for the prognostic relevance of the origin of TD, and although some histopathological characteristics showed good interobserver agreement, these are often non-specific. To optimize interobserver agreement, we recommend a binary classification of nodal versus extranodal tumor nodules which is based on prognostic evidence and yields good overall agreement.
Improved outcome in children compared to adolescents and young adults after allogeneic hematopoietic stem cell transplant for acute myeloid leukemia: a retrospective study from the Francophone Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC)
Authors: Pochon Cécile , Detrait Marie , Dalle Jean-Hugues , Michel Gérard , Dhédin Nathalie , Chalandon Yves , Brissot Eolia , Forcade Edouard , Sirvent Anne , Izzadifar-Legrand Faezeh , Michallet Mauricette , Renard Cécile , Yakoub-Agha Ibrahim , Gonzales Fanny , Bay Jacques-Olivier , Kanold Justyna , Cornillon Jérome , Bulabois Claude Eric , Angoso Marie , Nguyen Stéphanie , Balza Marie , Chevallier Patrice , Rialland Fanny , Bazarbachi Ali , Beguin Yves , Huynh Anne , Ménard Anne-Lise , Schneider Pascale , Neven Bénédicte , Paillard Catherine , Raus Nicole , Albuisson Eliane , Remen Thomas , Rubio Marie-Thérèse 
Published: September 2021

Abstract
Background
There are currently few data on the outcome of acute myeloid leukemia (AML) in adolescents after allogeneic HSCT. The aim of this study is to describe the outcome and its specific risk factors for children, adolescents and young adults after a first allogeneic HSCT for AML.

Methods
In this retrospective study, we compared the outcome of AML patients receiving a first allogeneic HSCT between 2005 and 2017 according to their age at transplantation’s time: children (< 15 years, n = 564), adolescent and post-adolescent (APA) patients (15–25 years, n = 647) and young adults (26–40 years; n = 1434).

Results
With a median follow-up of 4.37 years (min–max 0.18–14.73 years), the probability of 2-year overall survival (OS) was 71.4% in children, 61.1% in APA patients and 62.9% in young adults (p = 0.0009 for intergroup difference). Both relapse and non-relapse mortality (NRM) Cumulative Incidence (CI) estimated at 2 years were different between the age groups (30.8% for children, 35.2% for APA patients and 29.4% for young adults—p = 0.0254, and 7.0% for children, 10.6% for APA patients and 14.2% for young adults, p < 0.0001; respectively). Whilst there was no difference between the three groups for grade I to IV acute GVHD CI at 3 months, the chronic GVHD CI at 2 years was higher in APA patients and young adults (31.4% and 36.4%, respectively) in comparison to the children (17.5%) (p < 0.0001). In multivariable analysis, factors associated with death were AML cytogenetics (HR1.73 [1.29–2.32] for intermediate risk 1, HR 1.50 [1.13–2.01] for intermediate risk 2, HR 2.22 [1.70–2.89] for high cytogenetics risk compared to low risk), use of TBI ≥ 8 Grays (HR 1.33 [1.09–1.61]), disease status at transplant (HR 1.40 [1.10–1.78] for second Complete Remission (CR), HR 2.26 [1.02–4.98] for third CR and HR 3.07 [2.44–3.85] for active disease, compared to first CR), graft source (HR 1.26 [1.05–1.50] for Peripheral Blood Stem Cells compared to Bone Marrow) and donor age (HR 1.01 (1–1.02] by increase of 1 year).

Conclusion
Age is an independent risk factor for NRM and extensive chronic GVHD. This study suggests that APA patients with AML could be beneficially treated with a chemotherapy-based MAC regimen and bone marrow as a stem cells source.
The pericardium of Oikopleura dioica (Tunicata, Appendicularia) contains two distinct cell types and is rotated by 90 degrees to the left
Authors: Le Mai-Lee Van , Novosolov Maria , Huchon Dorothee , Stach Thomas 
Published: September 2021

AbstractThe planktonic Oikopleura dioica belongs to Tunicata, the probable sister taxon to Craniota, and might show plesiomorphic characters, conserved from the common lineage of Tunicata and Craniota. In O. dioica a pericardium in a position similar to other chordates but also to the heart and pericardium of craniates is found. Surprisingly, little is known about the ultrastructure of the pericardium in O. dioica. Here, we show based on electron microscopy that the pericardium is completely lined by a single layer of 16 epithelial cells: 6 epithelial myocardial cells on the left side of the pericardium and 10 peritoneal cells constituting the right side. One of the peritoneal cells, situated at the ventral border between peritoneal cells and myocardial cells has an extension that anchors the pericardium to the basal lamina beneath the latero-ventral epidermis. The primary body cavity of O. dioica appears quite uniformly clear in electron microscopic aspect but several sheets, resembling the basal lamina of the pericardium cross the larger spaces of the body cavity and connect to the pericardial basal lamina. This is the first detailed description of two distinct cell types in the epithelial lining of the pericardium of O. dioica. In comparison with other chordates, we conclude that two cell types can be reconstructed for the last common ancestor of Chordata at least. The position of the pericardium at the intersection of trunk and tail in combination with the basal-lamina like sheets spanning the hemocoel is probably of importance for the function of the circulation of the hemocoelic fluid. Similar to the tail, the axis of the pericardium is shifted through 90 degrees to the left as compared to the main body axis of the trunk and we infer that this shift is an apomorphic character of Appendicularia.
To get sick or not to get sick—Trichomonas infections in two Accipiter species from Germany
Authors: Merling de Chapa Manuela , Auls Susanne , Kenntner Norbert , Krone Oliver 
Published: September 2021

AbstractTrichomonosis caused by the flagellateTrichomonas gallinaeis one of the most important avian diseases worldwide. The parasite is localised in the oesophageal area of its host and mainly infects pigeon and dove species. During the last decade, a host expansion to passerine birds occurred, making the disease a potential threat for passerine predators as naïve host species. Here, we investigated the effect of the parasite on twoAccipiterspecies in Germany which show a comparable lifestyle but differ in prey choice, the Northern goshawk (Accipiter gentilis) mainly hunting pigeons and the Eurasian sparrowhawk (Accipiter nisus) mainly feeding on passerines. We genetically identified the parasite strains using the Fe-Hydrogenase gene as marker locus and compared the incidence of parasite presence and clinical signs of trichomonosis between nestlings of the twoAccipiterspecies. In total, we identified 14 strains, with nine strains unknown so far. There was a higher strain diversity and prevalence ofTrichomonasspp. in goshawks than sparrowhawks (42.4% vs. 21.2%) whereas sparrowhawks when being infected more often displayed clinical signs of trichomonosis than goshawks (37.1% vs. 6.1%). Even though sparrowhawks were mainly infected with the finch epidemic strain and genetic data indicated some variation between isolates, no correlation with virulence could be detected. All in all, goshawks seem to be better adapted toTrichomonas infections, whereas to sparrowhawks, this is a novel disease with more severe manifestations, from individual morbidity to a higher risk of population decline caused by trichomonosis.
Nutrigenetic variants and response to diet/lifestyle intervention in obese subjects: a pilot study
Authors: Franzago Marica , Di Nicola Marta , Fraticelli Federica , Marchioni Michele , Stuppia Liborio , Vitacolonna Ester 
Published: September 2021

AbstractAimsNutritional and lifestyle interventions can contribute to prevent and treat obesity and its complications; however, genetic background may influence the success of a therapy. The aim of this pilot study is to evaluate the effects of the interaction between nutrigenetic variants and nutritional intervention, as well as the changes in clinical parameters and the adherence to Mediterranean diet (MedDiet) and to physical activity, of 18 overweight or obese subjects affected by T2D or dysglycemia included in a nutritional program.MethodsThe subjects’ clinical parameters as well as their PREDIMED score and physical activity levels were recorded and compared at baseline, at 6 months and at the end of the intervention. Rs9939609 inFTO, rs17782313 nearMC4R, rs326 inLPL, rs16147 inNPY, rs2943641 nearIRS-1were genotyped.ResultsThe subjects carrying theAallele inFTOlost less weight (p = 0.022) and had a lower BMI decrease from baseline to 12 months (p-interaction = 0.047) thanTTcarriers. In addition, there was a significant PREDIMED score modification over time, according to genotypes forFTOrs9939609 (p = 0.025) andNPYrs16147 (p = 0.039), respectively.ConclusionsThese preliminary findings show a significant interaction between genetic variants and the PREDIMED score, suggesting that individuals carrying theFTOvariant may lose less weight than non-carriers through diet/lifestyle intervention.
Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands
Authors: Geißler Daniel , Nirmalananthan-Budau Nithiya , Scholtz Lena , Tavernaro Isabella , Resch-Genger Ute 
Published: September 2021

AbstractFunctional nanomaterials (NM) of different size, shape, chemical composition, and surface chemistry are of increasing relevance for many key technologies of the twenty-first century. This includes polymer and silica or silica-coated nanoparticles (NP) with covalently bound surface groups, semiconductor quantum dots (QD), metal and metal oxide NP, and lanthanide-based NP with coordinatively or electrostatically bound ligands, as well as surface-coated nanostructures like micellar encapsulated NP. The surface chemistry can significantly affect the physicochemical properties of NM, their charge, their processability and performance, as well as their impact on human health and the environment. Thus, analytical methods for the characterization of NM surface chemistry regarding chemical identification, quantification, and accessibility of functional groups (FG) and surface ligands bearing such FG are of increasing importance for quality control of NM synthesis up to nanosafety. Here, we provide an overview of analytical methods for FG analysis and quantification with special emphasis on bioanalytically relevant FG broadly utilized for the covalent attachment of biomolecules like proteins, peptides, and oligonucleotides and address method- and material-related challenges and limitations. Analytical techniques reviewed include electrochemical titration methods, optical assays, nuclear magnetic resonance and vibrational spectroscopy, as well as X-ray based and thermal analysis methods, covering the last 5–10 years. Criteria for method classification and evaluation include the need for a signal-generating label, provision of either the total or derivatizable number of FG, need for expensive instrumentation, and suitability for process and production control during NM synthesis and functionalization.Graphical abstract
Impacts of climate change and rising atmospheric CO2 on future projected reference evapotranspiration in Emilia-Romagna (Italy)
Authors: Ben Hamouda Ghaieth , Tomozeiu Rodica , Pavan Valentina , Antolini Gabriele , Snyder Richard L. , Ventura Francesca 
Published: September 2021

AbstractThe continuous increase of atmospheric CO2content mainly due to anthropogenic CO2emissions is causing a rise in temperature on earth, altering the hydrological and meteorological processes and affecting crop physiology. Evapotranspiration is an important component of the hydrological cycle. Thus, understanding the change in evapotranspiration due to global warming is essential for better water resources planning and management and agricultural production. In this study, the effect of climate change with a focus on the combined effect of temperature and elevated CO2concentrations on reference evapotranspiration (ETo) was evaluated using the Penman–Monteith equation. A EURO-CORDEX regional climate model (RCM) ensemble was used to estimate ETo in five locations in the Emilia-Romagna region (Northern Italy) during the period 2021–2050. Then, its projected changes in response to different CO2concentrations (i.e., 372 ppm and 550 ppm) under two Representative Concentration Pathways (RCP) scenarios (i.e., RCP4.5 and RCP8.5) were analyzed. Simulation results with both scenarios, without increasing CO2levels (372 ppm), showed that the annual and summertime ETo for all locations increased by an average of 4 to 5.4% with regard to the reference period 1981–2005, for an increase of air temperature by 1 to 1.5 °C. When the effect of elevated CO2levels (550 ppm) was also considered in combination with projected changes in temperature, changes in both annual and summer ETo demand for all locations varied from − 1.1 to 2.2% during the 2021–2050 period with regard to the reference period 1981–2005. This shows that higher CO2levels moderated the increase in ETo that accompanies an increase in air temperature.
Energy conservation under extreme energy limitation: the role of cytochromes and quinones in acetogenic bacteria
Authors: Rosenbaum Florian P. , Müller Volker 
Published: September 2021

AbstractAcetogenic bacteria are a polyphyletic group of organisms that fix carbon dioxide under anaerobic, non-phototrophic conditions by reduction of two mol of CO2 to acetyl-CoA via the Wood–Ljungdahl pathway. This pathway also allows for lithotrophic growth with H2 as electron donor and this pathway is considered to be one of the oldest, if not the oldest metabolic pathway on Earth for CO2 reduction, since it is coupled to the synthesis of ATP. How ATP is synthesized has been an enigma for decades, but in the last decade two ferredoxin-dependent respiratory chains were discovered. Those respiratory chains comprise of a cytochrome-free, ferredoxin-dependent respiratory enzyme complex, which is either the Rnf or Ech complex. However, it was discovered already 50 years ago that some acetogens contain cytochromes and quinones, but their role had only a shadowy existence. Here, we review the literature on the characterization of cytochromes and quinones in acetogens and present a hypothesis that they may function in electron transport chains in addition to Rnf and Ech.
Bennu and Ryugu: diamonds in the sky
Authors: Sabuwala Tapan , Chakraborty Pinaki , Shinbrot Troy 
Published: September 2021

AbstractRapidly spinning and loosely aggregated asteroids appear to take on diamond-shaped profiles, with elevated poles as well as equators. The evolutionary processes that form these characteristic shapes remain a matter of debate. In this paper, we propose a novel model, based on debris accretion, to explain these diamond-shaped profiles. We derive an analytic expression for the shapes of such rapidly spinning rubble piles based on the principle that as rubble is deposited it assumes a critical angle of repose. We show that this expression correctly reproduces diamond shaped profiles. We also conduct granular simulations of debris deposition and show that simulated shapes are in striking accord with both observations and analytical results. Our results suggest that non-uniform debris accumulation, which is overlooked in current models, may play a cardinal role in the formation of diamond-shaped asteroids.
The impact of inpatient bed capacity on length of stay
Authors: Walsh Brendan , Smith Samantha , Wren Maev-Ann , Eighan James , Lyons Seán 
Published: September 2021

Abstract
Objective
Large reductions in inpatient length of stay and inpatient bed supply have occurred across health systems in recent years. However, the direction of causation between length of stay and bed supply is often overlooked. This study examines the impact of changes to inpatient bed supply, as a result of recession-induced healthcare expenditure changes, on emergency inpatient length of stay in Ireland between 2010 and 2015.

Study design
We analyse all public hospital emergency inpatient discharges in Ireland from 2010 to 2015 using the administrative Hospital In-Patient Enquiry dataset. We use changes to inpatient bed supply across hospitals over time to examine the impact of bed supply on length of stay. Linear, negative binomial, and hospital–month-level fixed effects models are estimated.

Results
U-shaped trends are observed for both average length of stay and inpatient bed supply between 2010 and 2015. A consistently large positive relationship is found between bed supply and length of stay across all regression analyses. Between 2010 and 2012 while length of stay fell by 6.4%, our analyses estimate that approximately 42% (2.7% points) of this reduction was associated with declines in bed supply.

Conclusion
Changes in emergency inpatient length of stay in Ireland between 2010 and 2015 were closely related to changes in bed supply during those years. The use of length of stay as an efficiency measure should be understood in the contextual basis of other health system changes. Lower length of stay may be indicative of the lack of resources or available bed supply as opposed to reduced demand for care or the shifting of care to other settings.
Economic preferences and trade outcomes
Authors: Korff Alex , Steffen Nico 
Published: September 2021

AbstractIntegrating the Global Preference Survey (GPS) and its data of unique scope on national preference structures in patience, risk attitude and reciprocity into a gravity framework, this paper is the first to explore a potential influence on international trade outcomes of economic and social preferences in a unified setting. Adding to the evidence on preferences’ importance for aggregate outcomes, the authors find marked differences in trade flows and relationships, both on the country-level and between bilateral partners. Their main results suggest that countries differing in their willingness to behave negatively reciprocal tend to trade significantly less amongst each other due to the destabilizing effect of unexpected punishments. On the other hand, countries that are patient or risk-averse tend to shift towards exporting more differentiated goods as opposed to homogeneous goods and vice versa. We propose term and risk transformation considerations as the driving mechanisms for this relationship.