bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virus inactivation at moderately low pH varies with virus and buffer properties
Authors: Joshi Pratik U. , Meingast Christa L. , Xu Xuankuo , Holstein Melissa , Feroz Hasin , Ranjan Swarnim , Ghose Sanchayita , Li Zheng Jian , Heldt Caryn L. 
Published: December 2021

AbstractBackgroundVirus inactivation is a critical operation in therapeutic protein manufacturing. Low pH buffers are a widely used strategy to ensure robust enveloped virus clearance. However, the choice of model virus can give varying results in viral clearance studies. Pseudorabies virus (SuHV) or herpes simplex virus‐1 (HSV‐1) are frequently chosen as model viruses to demonstrate the inactivation for the herpes family.ResultsIn this study, SuHV, HSV‐1, and equine arteritis virus (EAV) were used to compare the inactivation susceptibility at pH 4.0 and 4°C. SuHV and HSV‐1 are from the same family, and EAV was chosen as a small, enveloped virus. Glycine, acetate, and citrate buffers at pH 4.0 and varying buffer strengths were studied. The inactivation susceptibility was found to be in the order of SuHV > HSV > EAV. The buffer effectiveness was found to be in the order of citrate > acetate > glycine. The smaller virus, EAV, remained stable and infectious in all the buffer types and compositions studied.ConclusionThe variation in inactivation susceptibility of herpes viruses indicated that SuHV and HSV cannot be interchangeably used as a virus model for inactivation studies. Smaller viruses might remain adventitiously infective at moderately low pH.
Infectious complications of immune checkpoint inhibitors in solid organ malignancies
Authors: Ross Justine Abella , Komoda Kellie , Pal Sumanta , Dickter Jana , Salgia Ravi , Dadwal Sanjeet 
Published: December 2021

AbstractBackgroundImmune checkpoint inhibitors (ICIs) are targeted cancer therapies regarded to have less toxicity than chemotherapy. Immune‐related adverse events (irAEs) of ICIs are well described in the literature but limited data exist on their infectious complications. The objective is to describe the spectrum and risk factors for developing serious infections in patients receiving ICIs.MethodsRetrospective review of patients with melanoma, renal cell carcinoma, or nonsmall‐cell lung cancer on nivolumab, pembrolizumab, and/or ipilimumab from January 1, 2017 to November 30, 2017. Exclusion: receipt of less than two ICI doses or history of other malignancy. Characteristics: age, sex, prior chemotherapy, steroid use, and temozolomide or infliximab use. Data identified from microbiology, radiography, serology, or physician note documentation. Serious infection is defined as infections requiring hospitalization and/or IV antibiotics from initiation of ICI until the end of the study period.ResultsOne hundred and eleven pts received ICIs. Suspected or confirmed bacterial infections occurred in 24% (27/111) with 8% (9/111) confirmed bacterial cultures. The overall serious infection rate was 14% (16/111) with 25% (4/16) confirmed bacterial cultures. Suspected or confirmed infection sites: genitourinary 20% (22/111), pneumonia 5% (7/111), skin/soft tissue 7% (8/111). Noninfectious pneumonitis (NIP) occurred in 5% (5/111). No association regarding the risk of infection between the type of malignancy and ICI used. Steroid use was the only risk factor significantly associated with serious infection: 12/16 (75%) on steroids versus 27/95 (28.4%) without steroid use (p = 0.0003).ConclusionThe rate of serious infection with ICI was higher in our study compared with previous reports of pts treated with melanoma. Infectious complications are encountered with ICIs and correlate with steroid use.
Synthesis of Novel Vindoline‐Chrysin Hybrids
Authors: Mayer Szabolcs , Nagy Nóra , Keglevich Péter , Szigetvári Áron , Dékány Miklós , Szántay Junior Csaba , Hazai László 
Published: December 2021

AbstractVinca alkaloids are well‐known microtubule targeting agents, which are used against some types of cancer. Vindoline is one of the monomeric Vinca alkaloids which does not have anti‐tumor effect, although its derivatives have serious impact on the field of these indole alkaloids. Chrysin is a secondary plant metabolite, which has broad‐spectrum biological activity, among others anticancer activity. Chrysin had shown synergic effect with several antiproliferative compounds (e. g., doxorubicin, cisplatin and ciglitazone), therefore, we attempted the synthesis of a novel vindoline‐chrysin hybrid molecule. However, in the first case a diphenylamine structure was isolated. The mechanism of the unexpected reaction was studied, and then the originally targeted hybrid was synthesized by a reverse route coupling. A further hybrid was produced using a different site of the molecule. The antitumor activities were determined against 60 human tumor cell lines (NCI60), where the aimed hybrid showed low micromolar GI50 values on most of the cell lines.
Effect of wheat gluten addition on the quality of thermal‐vacuum packaged Chinese steamed bread
Authors: Xiang Yuting , Dong Rui , Xu Shuya , Ren Tian , Hu Xinzhong 
Published: December 2021

AbstractBackground and objectivesIn order to investigate the effect of wheat gluten on thermal‐vacuum packaged Chinese steamed bread (CSB) quality during storage at room temperature, the wheat gluten addition levels of 0%, 1%, and 2% were set to study the effect on the protein structure, water mobility, starch retrogradation properties, and texture properties.FindingsWith the addition of wheat gluten, the β‐sheet content was increased and random coil content of thermal‐vacuum packaged CSB decreased. Wheat gluten enhanced the water holding capacity of CSB and restricted water migration, resulting in a lower content of bound water and higher content of less strongly bound water and free water according to the low‐field nuclear magnetic resonance (NMR). With the increase of wheat gluten, the diffraction peak intensity and relative crystallinity reduced. The elasticity and cohesion were higher, and the hardness, gumminess, chewiness, and resilience were lower than those in the control.ConclusionsThe results of this study revealed that the addition of wheat gluten can effectively inhibit moisture dispersion and starch retrogradation to delay the staling of CSB during storage.Significance and noveltyAs a natural food additive, wheat gluten can not only optimize protein structure and enhance nutritional value but also extend the shelf life of CSB. Therefore, these findings will promote industrial development of CSB.
Enteropathy‐associated T‐cell lymphoma manifesting as non‐specific abdominal pain: A case report highlighting the dangers of relying on Google Translate for clinical history taking
Authors: Leyden Deirbhile , Melby Niamh 
Published: December 2021

AbstractWe present the case of a 60‐year‐old man with non‐specific abdominal pain. We explore how communication between doctor and patient was challenged by a language barrier. We also consider how the ability to take an accurate clinical history differed between Google Translate and an accredited medical phone translation service.
Hyperinsulinemia/euglycemia and intravenous lipid emulsion therapy for the management of severe amlodipine toxicosis in a cat
Authors: Tinsman Audrey E. , Bellis Tara J. 
Published: December 2021

AbstractCalcium‐channel blockers (CCBs) are widely used in people and animals. Overdose can result in cardiovascular collapse and death. Hyperinsulinemia/euglycemia therapy (HIET) and intralipid therapy (ILT) are reported treatment options in people. This is the first report describing HIET and ILT as treatments for amlodipine toxicosis in a cat.
Management of lytic bone disease in lymphoplasmacytic lymphoma: A case report and review of the literature
Authors: Baksh Mizba , Jiang Liuyan , Bhatia Unnati , Alegria Victoria , Sher Taimur , Roy Vivek , Chanan‐Khan Asher , Ailawadhi Sikander , Parrondo Ricardo D. 
Published: December 2021

AbstractWaldenström macroglobulinemia (WM)/lymphoplasmacytic lymphoma (LPL) is often differentiated from myeloma based on the presence of lytic bone lesions (LBL). However, WM/LPL can present with LBL, and management is poorly understood. We describe a case of an 81‐year‐old woman with LPL who presented with LBL and was successfully treated with chemoimmunotherapy.
Cobalt‐Catalyzed Redox‐Neutral Sulfonylative Coupling from (Hetero)aryl Boronic Acids, Ammonium Salts and Potassium Metabisulfite
Authors: Zhang Yingying , Zhu Haibo , Fan Qiangwen , Yang Liu , Xie Zongbo , Le Zhang‐Gao 
Published: December 2021

AbstractAn efficient cobalt‐catalyzed redox‐neutral sulfonylative coupling to afford (hetero)aryl alkyl sulfones from boronic acids, ammonium salts and potassium metabisulfite has been realized. Commercially available and air‐stable CoCl2, in combination with phenanthroline ligand, is sufficient to achieve rapid and high‐yielding conversion of the reactants into the corresponding sulfones. This practical transformation proceeds smoothly through C−N bond cleavage under redox‐neutral catalytic conditions and shows broad functional‐group tolerance. Other carbon based electrophiles, including diaryliodonium salts, heteroaryl halides, and carbonates were compatible. Further transformation of aryl alkyl sulfones then allows conversion into olefins, alkenyl sulfones and halogenated sulfones, respectively, in a one‐pot process.
Bulk Mn2+ Doped 1D Hybrid Lead Halide Perovskite with Highly Efficient, Tunable and Stable Broadband Light Emissions
Authors: Jing Chang‐Qing , Yin Xu , Xiao Pan‐Chao , Gao Yu‐Jia , Wu Xiao‐Min , Yue Cheng‐Yang , Lei Xiao‐Wu 
Published: December 2021

AbstractMn2+ doped colloidal three‐dimensional (3D) lead halide perovskite nanocrystal (PNC) has attracted intensive research attention; however, the low exciton binding energy and fatal optical instability of 3D PNC seriously hinder the optoelectronic application. Therefore, it remains significant to explore new stable host perovskite with strongly bound exciton to realize more desirable luminescent property. In this work, we utilized bulk one‐dimensional (1D) hybrid perovskite of [AEP]PbBr5 ⋅ H2O (AEP=N‐aminoethylpiperazine) as structural platform to rationally optimize the luminescent property by a controllable Mn2+ doping strategy. Significantly, the series of Mn2+‐doped 1D [AEP]PbBr5 ⋅ H2O show enhanced energy transfer efficiency from the strongly bound excitons of host material to 3d electrons of Mn2+ ions, resulting in tunable broadband light emissions from weak yellow to strong red spectral range with highest photoluminescence quantum yield up to 28.41 %. More importantly, these Mn2+‐doped 1D perovskites display ultrahigh structural and optical stabilities in humid atmosphere, water and high temperature exceeding the conventional 3D PNC. Combined highly efficient, tunable and stable broadband light emissions enable Mn2+‐doped 1D perovskite as excellent down‐converting phosphor showcasing the potential application in white light emitting diode. This work not only provides a profound understanding of low‐dimensional perovskites but also opens a new way to rationally design high‐performance broadband light emitting perovskites for solid‐state lighting and displaying devices.
The characteristics of upper airway edema in hereditary and acquired angioedema with C1‐inhibitor deficiency
Authors: Balla Zsuzsanna , Andrási Noémi , Pólai Zsófia , Visy Beáta , Czaller Ibolya , Temesszentandrási György , Csuka Dorottya , Varga Lilian , Farkas Henriette 
Published: December 2021

AbstractBackgroundAngioedemas localized in the upper airway are potentially life threatening, and without proper treatment, they may lead to death by suffocation. Upper airway edemas (UAE) in bradykinin‐mediated angioedemas can even be the first symptoms of the disease.MethodsOur survey was performed with a retrospective long‐term follow‐up method from the medical history of 197 hereditary (C1‐INH‐HAE) and 20 acquired C1‐inhibitor deficiency (C1‐INH‐AAE), 3 factor XII and 3 plasminogen gene mutation (FXII‐HAE, PLG‐HAE) patients treated at our center between 1990 and 2020. The UAE group included edemas localized to the mesopharynx, hypopharynx, and larynx, as narrowing of these anatomical regions can lead to suffocation.Results98/197 C1‐INH‐HAE (47 families) and 13/20 C1‐INH‐AAE, 1/3 PLG‐HAE, 1/3 FXII‐HAE patients had experienced UAE at least once according to their medical history. In case of C1‐INH‐HAE patients, in 6/47 families who had undiagnosed ancestors had 13 members who died of suffocation. After the diagnosis, 1‐1 member of two families died of UAE. 44/64 C1‐INH‐HAE patients did not smoke, 20/64 did. The occurrence of UAE was significantly higher in smoker patients. We analyzed 7607 HAE attacks of 56/98 patients. Out of all attacks, the incidence of UAE in the C1‐INH‐HAE group was 4%, and 9.5% in the C1‐INH‐AAE group, respectively.ConclusionEarly diagnosis is key in bradykinin‐mediated angioedemas cases, since the patient must be provided with adequate treatment; and also it is essential to inform patients about the importance of avoiding the trigger factors and the early symptoms of UAE, as these measures could significantly decrease the incidence of lethal UAEs.
Nanoporous Pt(Au) Alloys for the Enhanced, Non‐enzymatic Detection of Hydrogen Peroxide under Biofouling Conditions
Authors: Khan Rezaul K. , Silva Tiago A. , Fatibello‐Filho Orlando , Collinson Maryanne M. , Farghaly Ahmed A. 
Published: December 2021

AbstractThe non‐enzymatic electrochemical sensing of hydrogen peroxide (H2O2) is described. This study utilized a 3D bicontinuous nanoporous platinum‐gold alloy‐based sensor (NP−Pt(Au) electrode), which has a morphology that resembles a molecular sieve. SEM and CV were used to evaluate the morphological and electrochemical characteristics of the NP−Pt(Au) electrodes. The cathodic voltammetric response of H2O2 was enhanced at the NP−Pt(Au) as compared to planar Pt electrodes. The nanoporous electrode was able to maintain the H2O2 voltammetric profile even in the presence of the biofouling agent, serum albumin. Its application as a non‐enzymatic sensor for the amperometric measurement of H2O2 was explored.
An insoluble α‐Ketoglutarate salt coated onto an inert high surface area solid support prevents autoxidation of neat citrus oil
Authors: Calandra Michael J. , Wang Ying , Dodson Jeff , Roberto Amador Joao 
Published: December 2021

AbstractTerpene hydroperoxides (THPs) have been reported to be skin sensitizers, so their elimination from citrus oils is desirable for consumer safety. Previously, we reported that treatment of citrus‐based perfumes with pyruvic acid and α‐ketoglutaric acid (AKG) or their salts was effective at removing existing THPs, measured by peroxide value (POV) titration. AKG salts, if soluble, were effective in neat citrus oil as well, but an insoluble AKG salt was only moderately effective. However, contact with an insoluble salt did prevent further autoxidation of the citrus oil. Herein, we report that coating an inert solid support with an insoluble AKG salt, made from either N‐methyl diethanolamine or 1‐[bis[3‐(dimethylamino)propyl]amino]‐2‐propanol, produced an easily handled device for insertion into a container of citrus oil; the device was highly effective at maintaining a low POV in fresh orange oil for an extended storage period. Such devices offer a promising mode of preventing autoxidation in fresh citrus oil raw materials.
Performance of three freely available methods for extracting white matter hyperintensities: FreeSurfer, UBO Detector, and BIANCA
Authors: Hotz Isabel , Deschwanden Pascal Frédéric , Liem Franziskus , Mérillat Susan , Malagurski Brigitta , Kollias Spyros , Jäncke Lutz 
Published: December 2021

AbstractWhite matter hyperintensities (WMH) of presumed vascular origin are frequently found in MRIs of healthy older adults. WMH are also associated with aging and cognitive decline. Here, we compared and validated three algorithms for WMH extraction: FreeSurfer (T1w), UBO Detector (T1w + FLAIR), and FSL's Brain Intensity AbNormality Classification Algorithm (BIANCA; T1w + FLAIR) using a longitudinal dataset comprising MRI data of cognitively healthy older adults (baseline N = 231, age range 64–87 years). As reference we manually segmented WMH in T1w, three‐dimensional (3D) FLAIR, and two‐dimensional (2D) FLAIR images which were used to assess the segmentation accuracy of the different automated algorithms. Further, we assessed the relationships of WMH volumes provided by the algorithms with Fazekas scores and age. FreeSurfer underestimated the WMH volumes and scored worst in Dice Similarity Coefficient (DSC = 0.434) but its WMH volumes strongly correlated with the Fazekas scores (rs = 0.73). BIANCA accomplished the highest DSC (0.602) in 3D FLAIR images. However, the relations with the Fazekas scores were only moderate, especially in the 2D FLAIR images (rs = 0.41), and many outlier WMH volumes were detected when exploring within‐person trajectories (2D FLAIR: ~30%). UBO Detector performed similarly to BIANCA in DSC with both modalities and reached the best DSC in 2D FLAIR (0.531) without requiring a tailored training dataset. In addition, it achieved very high associations with the Fazekas scores (2D FLAIR: rs = 0.80). In summary, our results emphasize the importance of carefully contemplating the choice of the WMH segmentation algorithm and MR‐modality.
Experimental Evidence on the Formation of Highly Strained 6,7‐Dihydroazeto[2,1‐b]oxazol‐3‐ium Derivatives as Reactive Intermediates
Authors: Xie Wenlai , Sun Simin , Xu Jiaxi 
Published: December 2021

AbstractThe formation of highly strained 6,7‐dihydroazeto[2,1‐b]oxazol‐3‐ium derivatives (4‐oxa‐1‐azabicyclo[3.2.0]hepta‐1(5),2‐dien‐1‐ium) as reactive intermediates was observed through the treatment of N‐2‐aryl‐2‐oxoethylazetidin‐2‐ones with phosphorus oxychloride as a dehydrating agent, affording 2‐vinyloxazoles as major products through elimination and 2‐(2‐chloroethyl)oxazoles as byproducts through nucleophilic substitution with the chloride anion. The current results provide important and useful information on the formation and existence of highly strained 6,7‐dihydroazeto[2,1‐b]oxazol‐3‐ium derivatives, which can be generated in situ and further undergo elimination and nucleophilic displacement immediately due to their high strain. 6,7‐Dihydroazeto[2,1‐b]oxazol‐3‐ium species are unstable and cannot be isolated from the reaction mixtures. They also provide an efficient method for the synthesis of 2‐vinyloxazole derivatives.
Circulating exosomal microRNAs as diagnostic and prognostic biomarkers in patients with diffuse large B‐cell lymphoma
Authors: Cao Di , Cao Xia , Jiang Yu , Xu Juan , Zheng Yuhuan , Kang Deying , Xu Caigang 
Published: December 2021

AbstractExosomal microRNAs (miRNAs) are potential biomarkers for a variety of tumors, but have not yet been studied in diffuse large B‐cell lymphoma (DLBCL). Here, we investigated the use of exosomal miRNAs in DLBCL diagnosis and prognosis. A total of 256 individuals, including 133 DLBCL patients, 94 healthy controls (HCs), and 29 non‐DLBCL concurrent controls (CCs), were enrolled. Exosomal miRNAs were profiled in the screening stage using microarray analysis, and miRNA candidates were confirmed in training, testing, and external testing stages using qRT‐PCR. Follow‐up information on the DLBCL patients was collected, and miRNAs were used to develop diagnostic and prognostic models for these patients. Five exosomal miRNAs (miR‐379‐5p, miR‐135a‐3p, miR‐4476, miR‐483‐3p, and miR‐451a) were differentially expressed between DLBCL patients and HCs with areas under the receiver operating characteristic curve (AUC) of 0.86, 0.90, and 0.86 for the training, testing, and external testing stages, respectively. Four exosomal miRNAs (miR‐379‐5p, miR‐135a‐3p, miR‐4476, and miR‐451a) were differentially expressed between patients with DLBCL and CCs, with an AUC of 0.78. One miRNA (miR‐451a) was significantly associated with both progression‐free survival (PFS) and overall survival (OS) of DLBCL patients, R analysis indicated the combination of miR‐451a with international prognostic index was a better predictor of PFS and OS for these patients. Our study suggests that subsets of circulating exosomal miRNAs can be useful noninvasive biomarkers for the diagnosis of DLBCL and that the use of circulating exosomal miRNAs improves the identification of patients with newly diagnosed DLBCL with poor outcomes.
School counseling college‐going culture: Counselors’ influence on students’ college‐going decisions
Authors: Bryan Julia , Kim Jungnam , Liu Chang 
Published: December 2021

AbstractThis study tested the conceptual framework of school counseling college‐going culture (SCCGC) using a national sample of 15,857 high school students from the High School Longitudinal Study 2009. We examined the relationship of SCCGC (i.e., counselor expectations and priorities, student–counselor contact for college–career counseling prior to 12th grade, college and career readiness activities, and constraints) to high school seniors’ college decisions (i.e., student–counselor contact for college admissions counseling in 12th grade, student–counselor contact for financial aid counseling in 12th grade, number of college applications, and enrollment in college). Multinomial and ordinal logistic regression analyses revealed that counselors’ expectations and priorities, student–counselor contact for college–career counseling prior to 12th grade, college application and financial aid help, and college information and Free Application for Federal Student Aid meetings were positively related while caseload was negatively related to students’ college decisions (after controlling for parent involvement, academic performance and aspirations, and student demographics).