bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA profiling of the pig periaqueductal grey (PAG) region reveals candidates potentially related to sex-dependent differences
Authors: Pawlina-Tyszko Klaudia , Oczkowicz Maria , Gurgul Artur , Szmatoła Tomasz , Bugno-Poniewierska Monika 
Published: January 2021

Abstract
Background
MicroRNAs indirectly orchestrate myriads of essential biological processes. A wide diversity of miRNAs of the neurodevelopmental importance characterizes the brain tissue, which, however, exhibits region-specific miRNA profile differences. One of the most conservative regions of the brain is periaqueductal grey (PAG) playing vital roles in significant functions of this organ, also those observed to be sex-influenced. The domestic pig is an important livestock species but is also believed to be an excellent human model. This is of particular importance for neurological research because of the similarity of pig and human brains as well as difficult access to human samples. However, the pig PAG profile has not been characterized so far. Moreover, molecular bases of sex differences connected with brain functioning, including miRNA expression profiles, have not been fully deciphered yet.

Methods
Thus, in this study, we applied next-generation sequencing to characterize pig PAG expressed microRNAs. Furthermore, we performed differential expression analysis between females and males to identify changes of the miRNA profile and reveal candidates underlying sex-related differences.

Results
As a result, known brain-enriched, and new miRNAs which will expand the available profile, were identified. The downstream analysis revealed 38 miRNAs being differentially expressed (DE) between female and male samples. Subsequent pathway analysis showed that they enrich processes vital for neuron growth and functioning, such as long-term depression and axon guidance. Among the identified sex-influenced miRNAs were also those associated with the PAG physiology and diseases related to this region.

Conclusions
The obtained results broaden the knowledge on the porcine PAG miRNAome, along with its dynamism reflected in different isomiR signatures. Moreover, they indicate possible mechanisms associated with sex-influenced differences mediated via miRNAs in the PAG functioning. They also provide candidate miRNAs for further research concerning, i.e., sex-related bases of physiological and pathological processes occurring in the nervous system.

Graphical abstract
Cross-talks among GBA mutations, glucocerebrosidase, and α-synuclein in GBA-associated Parkinson’s disease and their targeted therapeutic approaches: a comprehensive review
Authors: Behl Tapan , Kaur Gagandeep , Fratila Ovidiu , Buhas Camelia , Judea-Pusta Claudia Teodora , Negrut Nicoleta , Bustea Cristiana , Bungau Simona 
Published: January 2021

AbstractCurrent therapies for Parkinson’s disease (PD) are palliative, of which the levodopa/carbidopa therapy remains the primary choice but is unable to modulate the progression of neurodegeneration. Due to the complication of such a multifactorial disorder and significant limitations of the therapy, numerous genetic approaches have been proved effective in finding out genes and mechanisms implicated in this disease. Following the observation of a higher frequency of PD in Gaucher’s disease (GD), a lysosomal storage condition, mutations of glycosylceramidase beta (GBA) encoding glucocerebrosidase (GCase) have been shown to be involved and have been explored in the context of PD. GBA mutations are the most common genetic risk factor of PD. Various studies have revealed the relationships between PD and GBA gene mutations, facilitating a better understanding of this disorder. Various hypotheses delineate that the pathological mutations of GBA minimize the enzymatic activity of GCase, which affects the proliferation and clearance of α-synuclein; this affects the lysosomal homeostasis, exacerbating the endoplasmic reticulum stress or encouraging the mitochondrial dysfunction. Identification of the pathological mechanisms underlying the GBA-associated parkinsonism (GBA + PD) advances our understanding of PD. This review based on current literature aims to elucidate various genetic and clinical characteristics correlated with GBA mutations and to identify the numerous pathological processes underlying GBA + PD. We also delineate the therapeutic strategies to interfere with the mutant GCase function for further improvement of the related α-synuclein–GCase crosstalks. Moreover, the various therapeutic approaches such as gene therapy, chaperone proteins, and histone deacetylase inhibitors for the treatment of GBA + PD are discussed.
Charging device for wearable electromagnetic energy-harvesting textiles
Authors: Lee Hyewon , Roh Jung-Sim 
Published: January 2021

AbstractThe study aims to develop charging devices for wearable electromagnetic energy harvesting textiles (WEHT). Electromagnetic energy through human movement can be easily and naturally generated and is not significantly affected by environmental factors, however, the electric current generated by the electromagnetic method of human movement is difficult to efficiently charge. Three charging circuits for use with wearable electromagnetic energy-harvesting textiles were developed. The three types of charging circuits developed are rectifier, voltage doubler, and voltage quadrupler circuits. The performances of the developed circuits were evaluated in comparison with a normal storage circuit, in which the generated energy is stored immediately. The results show that storage energy was generated from the WEHT in all the developed circuits, and the charging efficiency improved as the simulated walking frequency increased. Energy generated from wearable electromagnetic energy harvesting textiles has the highest storage efficiency when charged with a rectifier circuit. The rectifying circuit method showed a charging rate twice that of a normal storage circuit. The charging speed of the rectifier circuit was faster to reach 3.7 V, the nominal maximum barrier voltage of the single-cell lithium-ion batteries used in portable devices, than the normal charging circuit. In the voltage multiplier circuit, the voltage drop generated in the circuit was large, so the charging efficiency was not superior to the normal circuit or rectifier circuit. In conclusion, it is most effective to use a rectifier circuit for charging portable electronic devices using the energy harvested by wearable electromagnetic energy harvesting textiles.
Tensile strength assay comparing the resistance between two different autologous platelet concentrates (leucocyte-platelet rich fibrin versus advanced-platelet rich fibrin): a pilot study
Authors: Pascoal Martim de Almeida Nóbrega Correia , dos Santos Nuno Bernardo Malta , Completo António Manuel Godinho , Fernandes Gustavo Vicentis de Oliveira 
Published: January 2021

AbstractBackgroundSince the leucocyte-platelet rich fibrin (L-PRF) was published in 2001, many studies have been developed, analyzing its properties, and also verifying new possibilities to improve it. Thereby, it emerges the advanced-platelet rich fibrin (A-PRF) with a protocol that optimizes the properties obtained by the L-PRF. Nonetheless, there is a gap in the literature to landmark the evolutive process concerning the mechanical properties in specific the resistance to tensile strength which consequently may influence the time for membrane degradation. Thus, this study had the goal to compare the resistance to the traction of membranes produced with the original L-PRF and A-PRF protocols, being the first to this direct comparison.FindingsThe harvest of blood from a healthy single person, with no history of anticoagulant usage. We performed the protocols described in the literature, within a total of 13 membranes produced for each protocol (n= 26). Afterward, the membranes were prepared and submitted to a traction test assessing the maximal and the average traction achieved for each membrane. The data were analyzed statistically using the unpairedttest. Regarding average traction, A-PRF obtained a value of 0.0288 N mm−2and L-PRF 0.0192 N mm−2(p< 0.05 using unpairedttest). For maximal traction, A-PRF obtained 0.0752 N mm−2and L-PRF 0.0425 N mm−2(p< 0.05 using unpairedttest).ConclusionWith this study, it was possible to conclude that indeed A-PRF has a significative higher maximal traction score and higher average traction compared to L-PRF, indicating that it had a higher resistance when two opposing forces are applied.
Granulocyte-colony stimulating factor-producing malignant phyllodes tumor of the breast: a rare case
Authors: Mizoguchi Kimihisa , Kaneshiro Kazuhisa , Kubo Makoto , Sadakari Yoshihiko , Kimura Yoshizo , Higaki Koichi , Ogata Toshiro , Taniguchi Masahiko 
Published: January 2021

Abstract
Background
Granulocyte-colony stimulating factor (G-CSF)-producing tumors can cause leukocytosis despite an absence of infection. G-CSF-producing tumors have been reported in various organs such as the lung, esophagus, and stomach but rarely in the breast. We report a case of G-CSF-producing malignant phyllodes tumor of the breast.

Case presentation
An 84-year-old woman visited our hospital complaining of a lump in her left breast without fever and pain. Laboratory tests revealed elevated white blood cell (WBC) count and G-CSF levels. A malignant tumor of the breast was diagnosed by core needle biopsy. We performed a total mastectomy and sentinel lymph node biopsy. The tumor was identified as a G-CSF-producing malignant phyllodes tumor. Within 7 days after surgery, the patient’s WBC count and G-CSF level had decreased to normal levels. She is alive without recurrence 13 months after surgery.

Conclusions
We encountered a rare case of G-CSF-producing malignant phyllodes tumor of the breast. PET–CT revealed diffuse accumulation of FDG in the bone. Phyllodes tumors need to be differentiated from bone metastasis, lymphoma, and leukemia. We must be careful to not mistake this type of tumor for bone marrow metastasis.
Common nutritional deficiency with unusual site of presentation
Authors: Balamurugesan Kandan , Ponprabha Rajangam , Davis Prem 
Published: January 2021

Abstract
Background
Pellagra is a nutritional disorder due to deficiency of vitamin B3 (niacin) which is characterized by dermatitis, dementia, diarrhea, and death. The most common site of dermatitis in pellagra is the Casal necklace; however, in this case, the presentation of dermatitis is in unusual sites. Therefore, mucocutaneous signs play a major role in diagnosing the condition. The clinical features of pellagra begin with gastrointestinal symptoms followed by neurological symptoms which are subtle and non-specific. But if misdiagnosed and untreated, it can progress to death from multiorgan failure.

Case presentation
A 46-year-old Indian farmer, chronic alcoholic of 20 years duration, presented to the hospital with the complaints of darkening and thickening of the skin over the left upper chest and shoulder, epigastric region, left arm and forearm, and right arm and forearm for a period of 4 months with mild cognitive decline. His blood parameters revealed moderate anemia with microcytic hypochromic blood picture and decreased MCV, MCH, and MCHC values. A diagnosis of pellagra was built clinically after exclusion of other causes and has been treated with nicotinamide and had drastic improvement on follow-up.

Conclusion
The unusual site of pellagra presentation is rare; however, the uncommon presentation of a common disease is always to be considered for diagnosing the disease at the early stage. The neuropsychiatric symptoms of the pellagra in a known case of chronic alcoholic are not well defined, and if untreated, it may progress to stupor and coma and ultimately death. Hence, appropriate diagnosis and earlier management helps in the prevention of various life-threatening complications of any disease.
Risk of miscarriage in women with psychiatric disorders
Authors: Magnus Maria C. , Havdahl Alexandra , Morken Nils-Halvdan , Wensaas Knut-Arne , Wilcox Allen J. , Håberg Siri E. 
Published: January 2021

BackgroundSome psychiatric disorders have been associated with increased risk of miscarriage. However, there is a lack of studies considering a broader spectrum of psychiatric disorders to clarify the role of common as opposed to independent mechanisms.AimsTo examine the risk of miscarriage among women diagnosed with psychiatric conditions.MethodWe studied registered pregnancies in Norway between 2010 and 2016 (n = 593 009). The birth registry captures pregnancies ending in gestational week 12 or later, and the patient and general practitioner databases were used to identify miscarriages and induced abortions before 12 gestational weeks. Odds ratios of miscarriage according to 12 psychiatric diagnoses were calculated by logistic regression.Miscarriage risk was increased among women with bipolar disorders (adjusted odds ratio 1.35, 95% CI 1.26–1.44), personality disorders (adjusted odds ratio 1.32, 95% CI 1.12–1.55), attention-deficit hyperactivity disorder (adjusted odds ratio 1.27, 95% CI 1.21–1.33), conduct disorders (1.21, 95% CI 1.01, 1.46), anxiety disorders (adjusted odds ratio 1.25, 95% CI 1.23–1.28), depressive disorders (adjusted odds ratio 1.25, 95% CI 1.23–1.27), somatoform disorders (adjusted odds ratio 1.18, 95% CI 1.07–1.31) and eating disorders (adjusted odds ratio 1.14, 95% CI 1.08–1.22). The miscarriage risk was further increased among women with more than one psychiatric diagnosis. Our findings were robust to adjustment for other psychiatric diagnoses, chronic somatic disorders and substance use disorders. After mutual adjustment for co-occurring psychiatric disorders, we also observed a modest increased risk among women with schizophrenia spectrum disorders (adjusted odds ratio 1.22, 95% CI 1.03–1.44).ConclusionsA wide range of psychiatric disorders were associated with increased risk of miscarriage. The heightened risk of miscarriage among women diagnosed with psychiatric disorders highlights the need for awareness and surveillance of this risk group in antenatal care.
First-Line Palliative Chemotherapy for Esophageal and Gastric Cancer: Practice Patterns and Outcomes in the General Population
Authors: Merchant Shaila J. , Kong Weidong , Gyawali Bishal , Hanna Timothy P. , Chung Wiley , Nanji Sulaiman , Patel Sunil V. , Booth Christopher M. 
Published: January 2021

PURPOSE: Clinical trials have shown that palliative chemotherapy (PC) improves survival in patients with incurable esophageal and gastric cancer; however, outcomes achieved in routine practice are unknown. We describe treatment patterns and outcomes among patients treated in the general population of Ontario, Canada. METHODS: The Ontario Cancer Registry was used to identify patients with esophageal or gastric cancer from 2007 to 2016, and data were linked to other health administrative databases. Patients who received curative-intent surgery or radiotherapy were excluded. Factors associated with the receipt of PC were determined using logistic regression. First-line PC regimens were categorized, and trends over time were reported. Survival was determined using the Kaplan-Meier method. RESULTS: The cohort included 9,848 patients; 22% (2,207 of 9,848) received PC. Patients receiving PC were younger (mean age, 63 v 74 years; P < .0001) and more likely male (71% v 65%; P < .0001). Thirty-seven percent of non-PC patients saw a medical oncologist in consultation. Over the study period, utilization of PC increased (from 11% in 2007 to 19% in 2016; P < .0001), whereas the proportion of patients receiving triplet regimens decreased (65% in 2007 to 56% in 2016; P = .04). In the PC group, the median overall and cancer-specific survival from treatment initiation was 7.2 months. CONCLUSION: One fifth of patients with incurable esophageal and gastric cancer in the general population receive PC. Median survival of patients treated in routine practice is inferior to that in clinical trials. Only one third of patients not treated with PC had consultation with a medical oncologist. Further work is necessary to understand low utilization of PC and medical oncology consultation in this patient population.
The Deiodinases: Their Identification and Cloning of Their Genes
Authors: Galton Valerie Anne , Larsen P Reed , Berry Marla J 
Published: January 2021

Abstract
In this minireview, we provide a historical outline of the events that led to the identification and characterization of the deiodinases, the recognition that deiodination plays a major role in thyroid hormone action, and the cloning of the 3 deiodinase genes. The story starts in 1820, when it was first determined that elemental iodine was important for normal thyroid function. Almost 100 years later, it was found that the primary active principle of the gland, T4, contains iodine. Once radioactive iodine became available in the 1940s, it was demonstrated that the metabolism of T4 included deiodination, but at the time it was assumed to be merely a degradative process. However, this view was questioned after the discovery of T3 in 1952. We discuss in some detail the events of the next 20 years, which included some failures followed by the successful demonstration that deiodination is indeed essential to normal thyroid hormone action. Finally, we describe how the 3 deiodinases were identified and characterized and their genes cloned.
Comparison of the Analgesic Duration of 0.5% Bupivacaine With 1:200,000 Epinephrine Versus 0.5% Ropivacaine Versus 1% Ropivacaine for Low-Volume Ultrasound-Guided Interscalene Brachial Plexus Block: A Randomized Controlled Trial
Authors: Safa Ben , Flynn Brendan , McHardy Paul G. , Kiss Alex , Haslam Lynn , Henry Patrick D. , Kaustov Lilia , Choi Stephen 
Published: January 2021

BACKGROUND:
Bupivacaine and ropivacaine are the preferred long-acting local anesthetics for peripheral nerve blocks as they provide prolonged analgesia in the postoperative period. No studies have directly compared the analgesic duration of these commonly used local anesthetics in the setting of low-volume ultrasound-guided interscalene block (US-ISB). This study was designed to determine which local anesthetic and concentration provides superior analgesia (duration and quality) for low-volume US-ISB.

METHODS:
Sixty eligible patients scheduled for arthroscopic shoulder surgery were randomized (1:1:1) to receive US-ISB (5 mL) with 0.5% bupivacaine with 1:200,000 epinephrine, 0.5% ropivacaine, or 1% ropivacaine. All individuals were blinded including study participants, anesthesiologists, surgeons, research personnel, and statistician. All participants received a standardized general anesthetic and multimodal analgesia. The primary outcome was duration of analgesia defined as the time from the end of injection to the time that the patients reported a significant increase in pain (>3 numeric rating scale [NRS]) at the surgical site.

RESULTS:
The mean duration of analgesia for 0.5% bupivacaine with 1:200,000 epinephrine, 0.5% ropivacaine, or 1% ropivacaine was 14.1 ± 7.4, 13.8 ± 4.5, and 15.8 ± 6.3 hours, respectively (analysis of variance [ANOVA], P = .51). There were no observed differences in analgesic duration or other secondary outcomes between the 3 groups with the exception of a difference in cumulative opioid consumption up to 20h00 on the day of surgery in favor of ropivacaine 0.5% over bupivacaine of minimal clinical significance.

CONCLUSIONS:
In the context of single-injection low-volume US-ISB, we have demonstrated a similar efficacy between equal concentrations of ropivacaine and bupivacaine. In addition, increasing the concentration of ropivacaine from 0.5% to 1% did not prolong the duration of US-ISB.
Migration and Democratization in Brazil: The Case of Electoral Participation and Competition
Authors: Gori Maia Alexandre , Lu Yao 
Published: January 2021

AbstractDeepening democratization in Brazil has coincided with sustained flows of domestic migration, which raises an important question of whether migration deepens or depresses democratic development in migrant-sending regions. Whereas earlier perspectives have viewed migration as a political “brain drain,” we contend that out-migration can generate resources that promote democratic processes back home. We investigate the role of migration in two aspects of democratization: electoral participation and competition. The analyses are based on spatial panel data models of mayoral election results across all municipalities between 1996 and 2012. The results show that migration increases electoral participation and competition in migrant-sending localities in Brazil. This study also identifies the sociopolitical context that conditions the impact of migration: the effect is most often present in the context of rural-urban migration and is more pronounced in sending localities with less democratic political structures. Moreover, using spatial network models, we find evidence for the transmission of political remittances from migration destination municipalities to origin municipalities. The present study extends the research on the migration-development nexus to the political arena, thus demonstrating the value of integrating demographic processes into explanations of political change.
Sex Differences in Cardiometabolic Health Indicators after HIIT in Patients with Coronary Artery Disease
Authors: WAY KIMBERLEY L. , VIDAL-ALMELA SOL , MOHOLDT TRINE , CURRIE KATHARINE D. , AKSETØY INGER-LISE AAMOT , BOIDIN MAXIME , CORNELISSEN VERONIQUE A. , JOA KYUNG-LIM , KEECH ANDREW , JAYO-MONTOYA JON ANDER , TAYLOR JENNA L. , FOURINER KARINE , REED JENNIFER L. 
Published: January 2021

ABSTRACT

Purpose
Cardiorespiratory fitness (CRF) is an independent predictor of mortality, and females typically achieve smaller improvements in CRF than males after exercise-based cardiac rehabilitation. High-intensity interval training (HIIT) has been shown to produce superior improvements in CRF than traditional cardiac rehabilitation, but the sex differences are unknown. The purpose of this systematic review and meta-analysis was to evaluate sex differences for changes in CRF and cardiometabolic health indicators after HIIT in adults with coronary artery disease (CAD).

Methods and Results
A systemic search of five electronic databases for studies examining the effect of HIIT on measured CRF and cardiometabolic health indicators in adults with CAD was performed. Data (published and unpublished) from 14 studies were included in the meta-analyses with approximately eightfold greater male than female participation (n = 836 vs n = 103). Males with CAD achieved a near-significant absolute improvement in CRF (mean difference [MD] = 1.07, 95% confidence interval [CI] = −0.08 to 2.23 mL·kg−1⋅min−1, P = 0.07) after HIIT when compared with control; there were insufficient data to conduct such an analysis in females. Significantly smaller improvements in CRF were experienced by females than males (MD = −1.10, 95% CI = −2.08 to −0.12 mL·kg−1⋅min−1, P = 0.03); there was no sex difference for the relative (percentage) change in CRF after HIIT. Females achieved significantly smaller reductions in body mass index (MD = −0.25, 95% CI = −0.03 to −0.47 kg·m−2, P = 0.02) and fasting blood glucose (MD = −0.38, 95% CI = −0.05 to −0.72, P = 0.03); no sex differences were observed for other cardiometabolic health indicators.

Conclusion
There are no sex differences for relative improvements in CRF after HIIT; however, females are greatly underrepresented in trials. Future studies should increase female participation and perform sex-based analyses to determine sex-specific outcomes following HIIT.