bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and Clinical Significance of Origin Recognition Complex Family in Breast Cancer
Authors: chen shaohua , Jin Ziyao , Xin Linfeng , Lv Lv , Zhang Xuemei , Gong Yizhen , Liu Jianlun 
Published: September 2021

Abstract
BackgroundThe aim of this study is to investigate the potential clinical and prognostic value, role and driving molecular mechanisms of the origin recognition complex family in breast cancer.Resultsata from Oncomine, TCGA, GEO and ULCAN showed that ORC1L and ORC6L were highly expressed in breast cancer tissues, while the expression of ORC5L was inconsistent and there was no significant difference in the expression of ORC2L, ORC3L and ORC4L. High expression of ORC1L and ORC6L were mainly Her2 overexpressed subtype, and their expression were negatively correlated with patient age and positively correlated with tumor size, but not with lymph node metastasis, distant metastasis, or tumor stage. Expression of ORC5L was also negatively correlated with age and positively correlated with lymph node metastasis, but not with breast cancer molecular subtype and tumor size. Expression of ORC1L and ORC5L had high diagnostic value, and ORC6L had the highest diagnostic value in breast cancer. ORC6L was an independent poor prognostic factor for overall survival of breast cancer patients. It was involved in cell cycle progression, cell senescence, epigenetic regulation and other biological functions, and may regulate signaling pathways such as NF-KB, TP53 and WNT in breast cancer. We also found that the expression of ORC6L was related to the increased infiltration of Th1/2 cell and Treg cell, and decreased infiltration of Mast cell and NK cell.ConclusionsORC1L and ORC6L are highly expressed in breast cancer tissues, of which ORC6L has high diagnostic value and is an independent poor prognostic factor for overall survival of breast cancer patients. ORC6L may be involved in the occurrence and progression of breast cancer by regulating cell cycle progression, promoting the activation of cancer signaling pathways, and influencing tumor immune cells infiltration.
GIS-based Analytical Modeling on Evaluating Impacts of Urbanization in Amman Water Resources, Jordan
Authors: Odeh Taleb , Mohammad Alsharifa Hind , Pradhanang Soni M , Ismail Mamoon , Rödiger Tino 
Published: September 2021

Abstract
Amman governorate is the largest governorate in term of population and urbanization in Jordan that is the third most water-scarce country worldwide. It has also limited water resources that were rapidly decreasing as results of groundwater over-pumping and climate changes that generates a serious water crisis. However, the population and urbanization focused on the Northwest of the governorate. The surface water and groundwater resources are available in the Northwest area as well. The overlaying between urbanization and population on one-hand and water resources on the other hand resulted in different environmental, hydrological, and hydrogeological problems. Our research investigated these problems using an integrated approach of remotes sensing and geographic information systems. Furthermore, our research suggested a spatial plan that would solve the conflict of urbanization impact on water resources in Amman. Accordingly, the catchment areas that span on the study area and their drainage network were defined.
Association of Intraoperative Cerebral and Somatic Tissue Oxygen Saturation with Postoperative Cognitive Dysfunction After Spine Open Surgery in Patients with Hypertension: An Observational Study
Authors: Guo Fei , Jia Shuaiying , Wang Qiyan , Liu Qinyu , Hu Mingquan , Wang Wenzhang , Liu Shijian , Li Qiang , Lu Bin , Zheng Yeying 
Published: September 2021

Abstract
Background: Intraoperative cerebral desaturations have been associated with worse neurological outcomes after supine surgery. However, it is not clear whether intraoperative somatic tissue oxygenation is more associated with postoperative cognitive dysfunction (POCD) than cerebral oxygenation in patients with hypertension after prone spine surgery.Methods: Patients with hypertension scheduled for spine open surgery were included from 2020 to 2021 in a single-center, prospective, observational study. Baseline both cerebral and somatic tissue oxygen saturation were measured in operating room before surgery. Cerebral and somatic tissue oxygen saturations were monitored continuously throughout surgery. The presence of POCD was assessed using the Mini-Mental Status Examination (MMSE). Association with POCD was evaluated with unadjusted analyses and multivariable logistic regression.Results: One hundred and one of 112 patients were included, and 28 (27.8%) developed POCD. None of the investigated SctO2 variables was predictive of POCD. On the contrary, the patients with POCD had a higher decrease in intraoperative absolute SstO2 decrease and relative SstO2 decrease compared with the patients without POCD (4.9%±3.8% vs. 3.6%±2.6%, P=0.037; 7.4%±5.6% vs. 5.3%±3.8%, P=0.036; respectively). Finally, three SstO2 parameters respectively were associated with POCD, including a higher absolute SstO2 decrease (OR, 1.223; 95%CI, 1.031-1.451; P=0.021), a higher absolute SstO2 decrease (OR, 1.138; 95%CI, 1.011-1.281; P=0.032) and falling below 90% of baseline SstO2 (OR, 11.388; 95%CI, 2.367-54.785; P=0.002), independent of ASA III, preoperative platelet and postoperative sepsis. Conclusions: Twenty-eight (27.8%) of 101patients developed POCD. Somatic tissue oxygenation has a stronger association with POCD than cerebral tissue oxygenation after spine open surgery in patients with hypertension.Clinical trial registration: ChiCTR1900028392. Registered 20 December 2019.
Descriptive Epidemiology of Injuries in Japanese Collegiate Men’s Basketball: 2013/2014 to 2019/2020
Authors: Sekine Yuta , Kamada Kotaro , Koyama Takeshi , Hoshikawa Seigo , Komatsu Takayuki 
Published: September 2021

Abstract
Background
Basketball is one of the most played sports in the world. However, only a few studies have examined the epidemiology of Japanese collegiate men’s basketball injuries. This study investigated the incidence of injury among Japanese collegiate men’s basketball from the 2013/2014 to the 2019/2020 seasons and identified unique patterns by comparing our data with the National Collegiate Athletic Association (NCAA) men’s basketball data.
Methods
Data from Japanese collegiate basketball teams of the Kanto Collegiate Basketball Federation Division I League during the 2013/2014 to 2019/2020 academic years (23 team seasons) were used in this study. Injury rates per 1000 athlete exposures (AEs) and injury proportions were calculated according to events, injury types, body parts, and common injury mechanisms. The injury rate ratio (IRR) with a 95% confidence interval (CI) was compared with that from the time-loss injury data from the NCAA’s previous reports.
Results
In total, 480 injuries during 97,515 AEs were reported, leading to an injury rate of 4.92 per 1,000 AEs (95% CI = 4.48–5.36). The overall injury rate was higher in Japan than in the NCAA (IRR = 1.55; 95% CI = 1.39–1.73). Lower extremity injuries occurred most frequently (73.5%). Ankle sprain was the most common injury in Japan, with higher injury rates than in the NCAA (IRR = 2.10; 95% CI = 1.72–2.57). The injury rate of concussion was lower in Japan than in the NCAA (IRR = 0.28; 95% CI = 0.14–0.55).
Conclusions
The rates of overall injury and ankle sprain were higher and that of concussion was lower in Japan than in the NCAA. These results might have been influenced by the environment and level of medical support surrounding Japanese basketball players and indicate an urgent need to improve the medical support systems to protect basketball players from injury.
Diagnose and Prediction of Different Machine Learning methods for Knee Osteoarthritis: a protocol for systematic review and meta-analysis
Authors: zhou yu , Mu Tong , Kong Xiaochuan , Zhang Le 
Published: September 2021

Abstract
Background: Knee osteoarthritis (OA) is a chronic and progressive joint disease with a higher contributor to global disability, mainly in the elderly and particularly in women. The available diagnostic approaches such as X-ray, computed tomography and magnetic resonance imaging have large precision errors and low sensitivity. Machine learning (ML) is the application of probabilistic algorithms to train a computational model to make predictions, it has great potential to become a valuable clinical diagnostic tool. This review aims to determine the diagnosis and prediction accuracy of different machine learning methods for Knee Osteoarthritis Methods: Two reviewers systematically searched Cochrane, PubMed, EMBASE, and Web of Science (last updated in June 2020) for eligible articles. To identify potentially missed publications, the reference lists of the final included studies were manually screened. Outcomes assessed were test characteristics such as accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve (ROC). We will use the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool to assess the risk of bias and applicability. Two independent reviewers will conduct all procedures of study selection, data extraction, and methodological assessment. Any disagreements will be consulted with a third reviewer. RevMan 5.3 software and Stata V15.0 will be used to pool data and to carry out the meta-analysis if it is possible. Results: This systematic review will provide a high-quality synthesis of machine learning for diagnose of knee Osteoarthritis from various evaluation aspects including accuracy, sensitivity, specificity and AUC.Conclusion: The findings of this systematic review will provide latest evidence of diagnosis and prediction of different machine learning for patients with knee Osteoarthritis.Ethics and dissemination: No individual patient data will be used in this study; thus, no ethics approval is needed.Systematic review registration: PROSPERO CRD: 42019133305
A New Bioreactor to Promote Follicular Growth With or Without Activin A
Authors: Jovet Cynthia , Fraison Eloise , Lornage Jacqueline , Morel-journel Nicolas , Gavoille Antoine , Laurent David , Montembault Alexandra , Fournier Cyrielle , Salle Bruno , Labrune Elsa 
Published: September 2021

Abstract
Background: The aim of the present study was to evaluate the effect of Activin A on the activation of in vitro folliculogenesis of human ovarian tissues with or without our new three-dimensional structure (3D-structure). Methods: Five fresh ovarian human tissues were cultured in vitro in 4 groups with 100ng/mL Activin A or without Activine A and within or without a 3D-structure for 20 or 22 days of in vitro culture. Follicular density and quality were evaluated, and follicular diameters were measured. Estradiol secretion was quantified. Proliferation and apoptosis through immunostaining were performed.Results: The proportion of primordial follicles was significantly reduced, and the proportion of primary and secondary follicles was significantly increased in all four groups (p<0.001). Antral cavities were observed in the four culture groups. Activin A supplementation did not significantly affect the follicular density, follicular quality, follicular growth, or estradiol secretion (p>0.05). The 3D-structure increased the density of primary follicles and decreased the estradiol secretion (p<0.001). Tissular proliferation was significantly lower in the 3D-structure group compared to the non-3D-structure group (p=0.008). Regarding tissular apoptosis, it was significantly higher in the Activin group compared to the non-Activin group (0.006). Conclusion: The presence of Activin A did not seem to play a key role in in vitro folliculogenesis activation. However, the results may indicate that the 3D-structure could be more physiological and could prevent a pejorative in vitro folliculogenesis flare-up.
The genetic basis of tail-loss evolution in humans and apes
Authors: Yanai Itai , Xia Bo , Zhang Weimin , Wudzinska Aleksandra , Huang Emily , Brosh Ran , Pour Maayan , Miller Alexander , Dasen Jeremy , Maurano Matthew , Kim Sang , Boeke Jef 
Published: September 2021

Abstract
The loss of the tail is one of the main anatomical evolutionary changes to have occurred along the lineage leading to humans and to the “anthropomorphous apes”1,2. This morphological reprogramming in the ancestral hominoids has been long considered to have accommodated a characteristic style of locomotion and contributed to the evolution of bipedalism in humans3–5. Yet, the precise genetic mechanism that facilitated tail-loss evolution in hominoids remains unknown. Primate genome sequencing projects have made possible the identification of causal links between genotypic and phenotypic changes6–8, and enable the search for hominoid-specific genetic elements controlling tail development9. Here, we present evidence that tail-loss evolution was mediated by the insertion of an individual Alu element into the genome of the hominoid ancestor. We demonstrate that this Alu element – inserted into an intron of the TBXT gene (also called T or Brachyury10–12) – pairs with a neighboring ancestral Alu element encoded in the reverse genomic orientation and leads to a hominoid-specific alternative splicing event. To study the effect of this splicing event, we generated a mouse model that mimics the expression of human TBXT products by expressing both full-length and exon-skipped isoforms of the mouse TBXT ortholog. We found that mice with this genotype exhibit the complete absence of a tail or a shortened tail, supporting the notion that the exon-skipped transcript is sufficient to induce a tail-loss phenotype, albeit with incomplete penetrance. We further noted that mice homozygous for the exon-skipped isoforms exhibited embryonic spinal cord malformations, resembling a neural tube defect condition, which affects ~1/1000 human neonates13. We propose that selection for the loss of the tail along the hominoid lineage was associated with an adaptive cost of potential neural tube defects and that this ancient evolutionary trade-off may thus continue to affect human health today.
Trait Impulsivity and Choice Impulsivity in Young Adults with Probable Binge Eating Disorder
Authors: Yan Wan-Sen , Zheng Dan-Hui , Liu Meng-Meng 
Published: September 2021

Abstract
Background

Binge Eating Disorder (BED) as a public health problem has been included in the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). Akin to addictive disorders, impulsivity-related neuropsychological constructs might be potentially involved in the onset and development of BED. However, it remains unclear which facets of impulsivity are connected to overeating and binge eating behaviors among non-clinical populations. The present study aimed to detect the relationship between impulsivity and BED both on the personality-trait and behavioral-choice levels in undiagnosed young adults.
Methods

Fifty-eight individuals with probable BED and 60 healthy controls, matched on age, gender, and educational level, were assessed by using a series of self-report measurements, including the Barratt Impulsiveness Scale (BIS-11), UPPSP Impulsive Behaviors Scale (UPPSP), Delay Discounting Test (DDT), and Probability Discounting Test (PDT).
Results

Multivariate analysis of variance models revealed that compared with healthy controls, the BED group showed elevated scores on the BIS-11 Attentional and Motor impulsiveness, and on the UPPSP Negative Urgency, Positive Urgency, and Lack of Perseverance. However, BED subjects had similar discounting rates on the DDT and PDT with healthy controls. Regression models found that Negative Urgency was the only positive predictor of BED.
Conclusions

These findings suggested that typical facets of trait impulsivity, which have been recognized in addictive disorders, were associated with BED in young adults, whereas choice impulsivity was not aberrantly seen in BED. This study might promote a better understanding of the pathogenesis of BED.
Identification, Functional Analysis and Preliminary Validation of Differentially Expressed Genes in Hyperacute Cerebral Infarction Patients
Authors: Li Qi , Li Lei , Yang Jia-Nan , Wei Qi 
Published: September 2021

Abstract
Background/AimsAt present, most studies on ischemic stroke and micro RNA are focused on 24h after stroke. Therefore, our experiment intends to analyze the differentially expressed genes in hyperacute cerebral infarction patients, and to analyze the relationship between the most significantly differentially expressed miRNA and clinical characteristics. MethodsDownload miRNA expression microarray and gene expression microarray from GEO database, screen differentially expressed genes and miRNA by bioinformatics method, and analyze their biological functions. The peripheral plasma of patients with hyperacute stroke was collected, the related miRNAs were extracted, and the relationship between them and clinical characteristics was analyzed. ResultsThe microarray included 39 hyperacute cerebral infarction patients patients and 33 healthy volunteers and 5 differentially expressed miRNAs and 1107 differentially expressed genes were obtained by analysis. MiR-3156-5p was selected to further analyze and verify the correlation with the clinical characteristics of patients. The results showed that the expression level of miR-3156-5p was significantly positively correlated with triglyceride level, and had a significant negative impact on stroke. The low level of miR-3156-5p in hyperacute cerebral infarction patients may be one of the initiating factors of neuroinflammation induced by ischemic stroke, and functional analysis and some existing experimental results also support this view. ConclusionsIn our study, a series of differentially expressed genes and differentially expressed miRNAs were screened by analyzing GEO data sets. In addition, the low expression of miR-3156-5p in hyperacute cerebral infarction patients may be one of the initiating factors of neuroinflammation induced by ischemic stroke.
First Comprehensive Report of Conventional Insecticide Resistance Status of the Mosquito Culex quinquefasciatus Say (Diptera: Culicidae) in Saudi Arabia
Authors: Hafez Abdulwahab M. 
Published: September 2021

Abstract
Background

The mosquito Culex quinquefasciatus Say is a vector of various fatal diseases including West Nile fever, filariasis, and Japanese encephalitis. The major approach in controlling Cx. quinquefasciatus to prevent associated disease transmission has focused on insecticides. However, because of overreliance on such measures, Cx. quinquefasciatus has developed resistance to these insecticides including organophosphates and pyrethroids.
Methods

We evaluated 10 commonly used conventional insecticides (five OPs and five pyrethroids) for toxicity/resistance in Cx. quinquefasciatus in adults and larvae in eight populations collected from the environs around Riyadh, Saudi Arabia.
Results

The LC50 values for the tested insecticides in adults did not differ significantly from those of a susceptible strain, except for bifenthrin, deltamethrin, cypermethrin, cyfluthrin, and fenitrothion against populations from Al-Nakhil, Al-Suwaidi, Al-Ghanemiya, Al-Masfa, and Al-Masanie regions. All Cx. quinquefasciatus adult populations exhibited susceptibility/low resistance to the tested organophosphates with resistance ratios of 0.23–0.80 for chlorpyrifos, 0.44–1.97 for malathion, 0.09–3.62 for fenitrothion, 0.05–2.10 for pirimiphos-methyl, and 0.11–0.93 for diazinon. The Cx. quinquefasciatus adult populations exhibited susceptibility/moderate resistance to the tested pyrethroids with resistance ratios of 0.59–2.56 for alpha-cypermethrin, 0.59–2.19 for bifenthrin, 0.60–7.07 for deltamethrin, 0.60–2.66 for cypermethrin, and 0.58–2.39 for cyfluthrin. In Cx. quinquefasciatus larva populations, susceptibility/low resistance to the tested organophosphates was observed with resistance ratios of 0.03–1.75 for chlorpyrifos, 0.19–3.42 for malathion, 0.11–2.78 for fenitrothion, and 0.08–1.15 for pirimiphos-methyl.
Conclusions

The susceptibility/low resistance of Cx. quinquefasciatus adults to OP and pyrethroid insecticides suggests that they still have good potency against the adults of this species in Riyadh, Saudi Arabia. These results provide a baseline for decision-making in integrated vector management programs for Cx. quinquefasciatus.
Application of right bronchial blocker under artificial pneumothorax in thoracoscopic enucleation of oesophageal leiomyoma
Authors: Zhao Luo , Gao Chao , He Jia , Han Zhijun , Li Li 
Published: September 2021

Abstract
Background

Oesophageal leiomyomas are one of the most common benign oesophageal tumours. This study summarized and analysed the clinical experience of thoracoscopic enucleation of oesophageal leiomyoma.
Methods

A total of 36 patients who underwent thoracoscopic enucleation of oesophageal leiomyoma at Peking Union Medical College Hospital between 2014 and 2020 were retrospectively analysed. Fifteen patients received single-lumen endotracheal intubation combined with a right bronchial blocker (SLET-B group), and twenty-one patients received double-lumen endotracheal intubation (DLET group). Clinical data, surgical variables, and postoperative complications were analysed and compared.
Results

The average tumour size in all patients was 4.31 ± 1.96 cm. The average tumour size among symptomatic patients was significantly larger than that among asymptomatic patients (5.08 ± 2.02 vs 3.71 ± 1.72, P < 0.05). Patients in the SLET-B group had a significantly larger average tumour size than patients in the DLET group (5.39 ± 2.13 vs 3.54 ± 1.42, P < 0.05). The SLET-B group had a significantly shorter operation time and shorter total hospital stay than the DLET group. No mucosal injury, conversion to thoracotomy, or other operative complications occurred in the SLET-B group. In the follow-up, no recurrence, dysphagia, or regurgitation was found in any of the patients.
Conclusions

Compared with traditional double-lumen intubation, artificial pneumothorax-assisted single-lumen endotracheal intubation combined with a bronchial blocker for thoracoscopic oesophageal leiomyoma enucleation can achieve complete removal of larger tumours, with fewer complications and shorter hospital stays.
Eating disorders as a repercussion of sexual assault, a consequence to consider
Authors: Malet-Karas Aurore , Bernard-Wallendorf Delphine , Piet Emmanuelle , Bertin Eric 
Published: September 2021

Abstract
Purpose

This study aims at clarifying the links between sexual violence and eating disorders (EDs).

Methods

In a sample of 12638 victims of sexual violence, we analyzed the situation of 546 victims that declared having developed ED. We assessed the characteristics of the assault (age, type of aggression) and the medical consequences (PTSD, depression, suicide attempts, anxiety disorders …).

Results

ED prevalence was 4.3% in the victim sample. The age of the first assault in ED victims was significantly lower than that of the whole population (12y vs 16 y for median; p<0.001). A much higher prevalence of sexual assault consequences was present in victims developing ED with odd ratios (OR) for: self-mutilation (OR = 11.5 [8.29-15.95], p<0.001); depression (OR=5.7 [4.81-6.86], p<0.001); self-medication (OR = 5.3 [3.86-7.19], p<0.001); suicide attempts (OR =4.5 [3.59-5.67], p<0.001); Post-traumatic stress disorder (OR = 3.8 [2.99-4.78], p<0.001) ; anxiety troubles (OR = 5.2 [4.11-6.47], p<0.001); alcoholism (OR =4.0 [2.81-5.58], p<0.001).

Conclusion

This study confirms the link between ED and sexual violence, especially in childhood, leading to severe psychological consequences. In this context, ED should be envisaged as a coping strategy accompanying emotional dysregulation due to traumatic events, and be treated as such.