bluuebunny/crossref_metadata_2025_split huggingface dataset:
Economic efficiency of the influence of the adaptogen “Epin-extra” on growth, development, yield of cucumbers in film greenhouses of Central Yakutia
Authors: Afanasyeva S. R. , Olesova M. M. , Platonova A. Z. , Sidorova M. P. 
Published: April 2021

Relevance. In peasant-farm conditions and conditions of subsidiary plots, with a wide variety of growing times in greenhouses, which are very different in equipment, heating and use, it is necessary to clarify the agro-ecological terms and introduce regulators of the growth and development of cucumber plants. The use of the adaptogen increases the yield. The aim of the research was to consider the economic efficiency from the use of the adaptogen “Epin-extra” on the growth, development and productivity of various varieties of cucumbers when grown in spring-summer film greenhouses of the Megino-Kangalassky ulus of the Republic of Sakha (Yakutia). Research methods were used according to the methods of the State Commission for Testing and Protection of Breeding Achievements of the Russian Federation [7, 8, 9]. The calculation of economic efficiency was carried out on the basis of the financial costs included in the technological map and the actual costs per 1 hectare according to the method of S.V. Melnikov.Results. As a result of the research it was found that with a two-fold treatment with a solution of Epin-extra, the development of the main phenological phase of flowering is accelerated for 6 days from control (without treatment with an adaptogen). At the same time, the prevalence of such diseases as ascochito decreases to 1.7%, and the prevalence of root rot is 2.5-3.2 times lower with 2-fold processing, depending on the hybrids. The use of the Epin-extra adaptogen solution increases the quality of products -marketability and weight of cucumber fruits. Due to the use in small volumes, ease of use, the Epin-extra solution increases profitability up to 58.1% with double processing on the Stella F1 hybrid and up to 46.4% in the Maisky F1 hybrid.
“The little lights in this dark tunnel”: Emotional support of nurses working in COVID-19 acute care hospital environments
Authors: Lapum Jennifer , Nguyen Megan , Lai Sannie , McShane Julie , Fredericks Suzanne 
Published: April 2021

Background: Working on the frontlines of hospitals during the COVID-19 pandemic has been challenging and distressing for nurses. The troublesome nature of these emotions have surfaced because of uncharted territory related to this virus, compromised work conditions, unfavourable patient outcomes, and the witnessing of suffering and loss. Although there has been renewed emphasis on how to emotionally support nurses, the nature of support needed is somewhat unknown considering that healthcare professionals have not experienced a pandemic of this magnitude in their lifetime. Aim: We explored how nurses were emotionally supported and how they can be better supported while working in COVID-19 acute care hospital environments. Methods: In this narrative study, semi-structured interviews were conducted with 20 registered nurses working in hospitals in the Greater Toronto Area and working on units caring for COVID-19+ patients. Results and Conclusions: Our findings reflected the organic emergence of support, intentional forms of support, and the social justice nature of support. It is important for hospital and government leaders to employ a multifold approach to emotionally support nurses. These supports include information transparency, visible presence of leadership, and recognition of nurses’ contributions. While emotionally supporting nurses, these types of resources can act as “little lights in this dark tunnel” of COVID-19 and illuminate a path forward. Implications: Some strategies relevant to clinical practice include regular rounding of units by leaders, and transparent communication about information and resources. Other strategies are on-site psychological support and legitimate support of mental health sick days as well as lobbying governments for financial compensation for the risky work involved in being a frontline provider and appropriate provision of personal protective equipment.
Glycemic profile, inflammatory markers, biochemical and histopathological parameters of mice fed with fermented dairy beverage with clove essential oil
Authors: Souza Keicy Sandy Silvestre de , Almeida Anna Christina de , Oliveira Stephanie Pedrosa de , Brandi Igor Viana , Santos Sérgio Henrique Sousa , Fonseca Francine Souza Alves , Santos Eliane Macedo Sobrinho , Oliveira Janaína Ribeiro , Freitas Daniela Fernanda de , Lelis Deborah de Farias , Moraes Daniel Silva , Silva João Matheus de Almeida , Souza Cintya Neves , Farias Paula Karoline Soares , Porto Raphael Rodrigues , Santos Hércules Otacilio 
Published: April 2021

Previous studies have shown that the clove essential oil concentration of 200 mg/kg did not present toxicity and histopathological changes in the liver. Based on the evidence presented in this study, we decided to evaluate in vivo the ability of a previously developed fermented dairy beverage, tested only in vitro, in which the standard chemical preservative, potassium sorbate, was replaced by clove essential oil (2 μl/ml), to be a functional food. To this end, twenty-four male Swiss mice were divided into three groups and submitted insulin sensitivity (IST) and glucose tolerance (GTT) testing, evaluation of physiological and biochemical parameters, histopathological analysis and quantification of the expression of anti-inflammatory and pro-inflammatory cytokines in the liver, after administering daily of the fermented dairy beverage with or without clove essential oil by gavage during 30 days. The group that received the fermented dairy beverage with clove essential oil showed a lower glycemic level in GTT, a higher sensitivity to insulin and a higher glucose decay constant rate (Kitt), with relation to the other groups. In the evaluation of physiological parameters there were no signs of toxicity in the mice during the experimental period. The biochemical parameters, histopathological analysis and expression of anti-inflammatory (IL-10) and pro-inflammatory (IL1β, IL6 e TNFα) cytokines in the liver of the mice, was not significantly affected by the treatment. These results corroborated by bioinformatics analysis demonstrate that the fermented dairy beverage with clove essential oil it can function as a substitute for conventional chemical preservatives and reduce glycemic levels.
Rugoscopia palatina e seus desafios na identificação humana: uma revisão integrativa
Authors: Neves Isabelle Silva Ramos das , Santiago Adriana Paula de Andrade da Costa e Silva , Silva Maria Izabel Teixeira da , Oliveira Evellyn Rayane Martins de 
Published: April 2021

Esta revisão integrativa teve por objetivo verificar como a identificação da Odontologia Legal, por meio das rugas palatinas, tem se apresentado na literatura científica e quais as limitações para o seu uso em perícias post mortem. O que demandou em uma busca sob os seguintes descritores: Rugoscopia palatina, Palatoscopia e Odontologia forense, nas bases de dados da PubMed, BVS (Medline e Lilacs), BBO e Scielo, para artigos publicados entre 2010 e 2019. Foram selecionados os que se apresentavam disponíveis com texto completo e gratuito, em idiomas de inglês e português, cuja conclusão estivesse de acordo com o propósito deste estudo, deixando de fora os artigos que tratassem de estudos relacionados a perícia no vivo ou ante mortem, bem como os repetidos. Foram contabilizados um total de 58 artigos, sendo que destes, 7 artigos atendiam por completo aos critérios de inclusão/exclusão. Estes tratavam de revisões de literatura narrativa, sistemática e meta-análise, relatos de caso pericial e revisão de relatos de caso. Pode-se concluir que a rugoscopia palatina se apresenta na literatura científica como um método útil como guia na identificação humana, normalmente complementar, e ainda com possibilidade de transmitir diversas informações. Porém, não deve ser utilizada como único método de identificação da vítima, posto que também apresenta várias limitações, as quais podem dificultar ou mesmo inviabilizar o procedimento identificatório.
Inhibition of IL-6 in the LCWE Mouse Model of Kawasaki Disease Inhibits Acute Phase Reactant Serum Amyloid A but Fails to Attenuate Vasculitis
Authors: Porritt Rebecca A. , Chase Huizar Carol , Dick Edward J. , Kumar Shyamesh , Escalona Renee , Gomez Angela C. , Marek-Iannucci Stefani , Noval Rivas Magali , Patterson Jean , Forsthuber Thomas G. , Arditi Moshe , Gorelik Mark 
Published: April 2021

ObjectiveKawasaki disease (KD) is the most common cause of acquired pediatric heart disease in the developed world. 10% of KD patients are resistant to front-line therapy, and no interventions exist to address secondary complications such as myocardial fibrosis. We sought to identify proteins and pathways associated with disease and anti-IL-1 treatment in a mouse model of KD.MethodsVasculitis was induced via Lactobacillus casei cell wall extract (LCWE) injection in 5-week-old male mice. Groups of mice were injected with LCWE alone, LCWE and IL-1 receptor antagonist anakinra, or saline for controls. Upper heart tissue was assessed by quantitative mass spectrometry analysis. Expression and activation of STAT3 was assessed by immunohistochemistry, immunofluorescence and Western blot, and IL-6 expression by RNA-seq and ELISA. A STAT3 small molecular inhibitor and anti-IL-6R antibody were used to evaluate the role of STAT3 and IL-6 in disease development.ResultsSTAT3 was highly expressed and phosphorylated in cardiac tissue of LCWE-injected mice, and reduced following anakinra treatment. Il6 and Stat3 gene expression was enhanced in abdominal aorta of LCWE-injected mice and reduced with Anakinra treatment. IL-6 serum levels were enhanced in LCWE-injected mice and normalized by anakinra. However, neither inhibition of STAT3 nor blockade of IL-6 altered disease development.ConclusionProteomic analysis of cardiac tissues demonstrates differential protein expression between KD-like, control and anakinra treated cardiac tissue. STAT3 and IL-6 were highly upregulated with LCWE and normalized by anakinra treatment. However, both STAT3 and IL-6 were dispensable for disease development indicating they may be bystanders of inflammation.
The First Contact of Human Dendritic Cells With Trypanosoma cruzi Reveals Response to Virus as an Unexplored Central Pathway
Authors: Gil-Jaramillo Natalia , Rocha Amanda Pereira , Raiol Tainá , Motta Flávia Nader , Favali Cecília , Brigido Marcelo M. , Bastos Izabela M. D. , Santana Jaime M. 
Published: April 2021

Chagas disease is a debilitating and neglected disease caused by the protozoanTrypanosoma cruzi. Soon after infection, interactions amongT. cruziand host innate immunity cells can drive/contribute to disease outcome. Dendritic cells (DCs), present in all tissues, are one of the first immune cells to interact withTrypanosoma cruzimetacyclic trypomastigotes. Elucidating the immunological events triggered immediately after parasite-human DCs encounter may aid in understanding the role of DCs in the establishment of infection and in the course of the disease. Therefore, we performed a transcriptomic analysis of a 12 h interaction betweenT. cruziand MoDCs (monocyte-derived DCs) from three human donors. Enrichment analyses of the 468 differentially expressed genes (DEGs) revealed viral infection response as the most regulated pathway. Additionally, exogenous antigen processing and presentation through MHC-I, chemokine signaling, lymphocyte co-stimulation, metallothioneins, and inflammasome activation were found up-regulated. Notable, we were able to identify the increased gene expression of alternative inflammasome sensors such as AIM2, IFI16, and RIG-I for the first time in aT. cruziinfection. Both transcript and protein expression levels suggest proinflammatory cytokine production during earlyT. cruzi-DCs contact. Our transcriptome data unveil antiviral pathways as an unexplored process duringT. cruzi-DC initial interaction, disclosing a new panorama for the study of Chagas disease outcomes.
Maintenance Therapy With Everolimus for Subependymal Giant Cell Astrocytoma in Patients With Tuberous Sclerosis – Final Results From the EMINENTS Study
Authors: Bobeff Katarzyna , Krajewska Karolina , Baranska Dobromila , Kotulska Katarzyna , Jozwiak Sergiusz , Mlynarski Wojciech , Trelinska Joanna 
Published: April 2021

The aim of this EMINENTS prospective, single-center, open-label, single-arm study was to evaluate the cumulative efficacy and safety of reduced doses of everolimus (maintenance therapy) in patients with tuberous sclerosis and subependymal giant cell astrocytoma (SEGA).Methods: The trial included 15 patients who had undergone at least 12 months of treatment with a standard everolimus dose. The dose of everolimus was reduced to three times a week, with a daily dose as in standard regimen. Data of 14 patients were analyzed. SEGA volume (SV) was evaluated at study entry and subsequent time points by an experienced radiologist. Adverse events (AEs) noted during maintenance therapy were compared to the AEs of standard dose period.Results: Patients were followed over a mean duration 58.37 months (95%CI: 45.95–70.78). The differences in SEGA volume between subsequent time points (0, 3, 6,12, 18, 24, 36, 48, and 60 months) were not statistically significant (p = 0.16). At the end of the study, 7 out of 10 patients had stable SEGA volume. No clinical symptoms of progression were observed in any patients. No patient or tumor-related risk factors of progression were identified. Regarding AEs, infections (stomatitis, bronchitis, diarrhea) and laboratory abnormalities (neutropenia, anemia, hyperglycemia) occurred less frequently during maintenance therapy compared to the standard dose regimen.Conclusions: Final results from EMINENTS study confirm that maintenance therapy with everolimus might represent a rational therapeutic option for patients TSC and SEGA after effective full dose treatment. It could be an option for patients who experienced everolimus-related AEs, instead of discontinuation of therapy. Careful evaluation of possible progression, especially concerning first six months of maintenance therapy should be advised.Clinical Trial Registration:www.drks.de, identifier DRKS00005584.
Effects of Transcranial Direct Current Stimulation Combined With Physical Training on the Excitability of the Motor Cortex, Physical Performance, and Motor Learning: A Systematic Review
Authors: Wang Baofeng , Xiao Songlin , Yu Changxiao , Zhou Junhong , Fu Weijie 
Published: April 2021

Purpose: This systematic review aims to examine the efficacy of transcranial direct current stimulation (tDCS) combined with physical training on the excitability of the motor cortex, physical performance, and motor learning.Methods: A systematic search was performed on PubMed, Web of Science, and EBSCO databases for relevant research published from inception to August 2020. Eligible studies included those that used a randomized controlled design and reported the effects of tDCS combined with physical training to improve motor-evoked potential (MEP), dynamic posture stability index (DPSI), reaction time, and error rate on participants without nervous system diseases. The risk of bias was assessed by the Cochrane risk of bias assessment tool.Results: Twenty-four of an initial yield of 768 studies met the eligibility criteria. The risk of bias was considered low. Results showed that anodal tDCS combined with physical training can significantly increase MEP amplitude, decrease DPSI, increase muscle strength, and decrease reaction time and error rate in motor learning tasks. Moreover, the gain effect is significantly greater than sham tDCS combined with physical training.Conclusion: tDCS combined with physical training can effectively improve the excitability of the motor cortex, physical performance, and motor learning. The reported results encourage further research to understand further the synergistic effects of tDCS combined with physical training.
Acute Recreational Noise-Induced Cochlear Synaptic Dysfunction in Humans With Normal Hearing: A Prospective Cohort Study
Authors: Wang Qixuan , Yang Lu , Qian Minfei , Hong Yingying , Wang Xueling , Huang Zhiwu , Wu Hao 
Published: April 2021

ObjectivesThe objective of the study was to identify the acute high-intensity recreational noise-induced effects on auditory function, especially the cochlear synaptopathy-related audiological metrics, in humans with normal hearing.MethodsThis prospective cohort study enrolled 32 young adults (14 males and 18 females); the mean age was 24.1 ± 2.4 years (ranging from 20 to 29). All participants with normal hearing (audiometric thresholds ≤25 dB HL at frequencies of 0.25, 0.5, 1, 2, 3, 4, 6, and 8 kHz for both ears) had already decided to participate in the outdoor music festival. Participants were asked to measure the noise exposure dose and complete auditory examinations, including the air-conduction pure-tone audiometry (PTA), distortion product otoacoustic emission (DPOAE), contralateral suppression (CS) on transient evoked otoacoustic emission (TEOAE), auditory brainstem response (ABR) test and Mandarin Hearing in Noise Test (MHINT), at baseline and 1 day and 14 days after music festival noise exposure.ResultsThe mean time of attending the music festival was 7.34 ± 0.63 h (ranging from 6.4 to 9.5), the mean time-weighted average (TWA) of noise exposure dose was 93.2 ± 2.39 dB(A) (ranging from 87.9 to 97.7). At neither 1 day nor 14 days post exposure, there were no statistically significant effects on PTA thresholds, DPOAE amplitudes, CS on TEOAEs, or MHINT signal-to-noise ratios (SNRs) of acute outdoor music festival noise exposure, regardless of sex. While the ABR wave I amplitudes significantly decreased at 1 day after exposure and recovered at 14 days after exposure, the exposed/unexposed ABR wave I amplitude ratio was significantly correlated with MHINT SNR change at 1 day after exposure, although it was not correlated with the noise exposure dose.ConclusionIn young adults with normal hearing, we found the self-compared decrement of ABR wave I amplitudes at 1 day post acute recreational noise exposure at high intensity, which also contributes to the change in speech perceptual ability in noisy backgrounds. This study indicated that auditory electrophysiological metric changes might be a more sensitive and efficient indicator of noise-induced cochlear synaptic dysfunction in humans. More attention should be paid to the recreational noise-induced cochlear synaptopathy and auditory perceptual disorder.
RETRACTED: LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway
Authors: Zhang Jie , Jiang Pinping , Wang Shoyu , Cheng Wenjun , Fu Shilong 
Published: April 2021

Aims: A growing number of studies have unveiled that long non-coding RNA (lncRNA) is conductive to cervical cancer (CC) development. However, the effect of LIPE-AS1 is remained to be studied in CC.Main Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was employed to measure LIPE-AS1 expression in CC tissues and the adjacent normal tissues. Additionally, we conducted gain- and loss-of functional experiments of LIPE-AS1 and adopted CCK8 assay, BrdU assay, and in vivo tumor formation experiment to test the proliferation of CC cells (HCC94 and HeLa). Besides, the apoptosis, invasion, and epithelial-mesenchymal transformation (EMT) of CC cells were estimated using flow cytometry, transwell assay, and western blot, respectively. Further, LIPE-AS1 downstream targets were analyzed through bioinformatics, and the binding relationships between LIPE-AS1 and miR-195-5p were verified via dual-luciferase activity experiment and RNA Protein Immunoprecipitation (RIP) assay. Moreover, rescue experiments were conducted to confirm the effects of LIPE-AS1 and miR-195-5p in regulating CC development and the expressions of MAPK signaling pathway related proteins were detected by RT-PCR, western blot, and immunofluorescence.Key Findings: LIPE-AS1 was over-expressed in CC tissues (compared to normal adjacent tissues) and was notably related to tumor volume, distant metastasis. Overexpressing LIPE-AS1 accelerated CC cell proliferation, migration and EMT, inhibited apoptosis; while LIPE-AS1 knockdown had the opposite effects. The mechanism studies confirmed that LIPE-AS1 sponges miR-195-5p as a competitive endogenous RNA (ceRNA), which targets the 3′-untranslated region (3′-UTR) of MAP3K8. LIPE-AS1 promoted the expression of MAP3K8 and enhanced ERK1/2 phosphorylation, which were reversed by miR-195-5p.Significance: LIPE-AS1 regulates CC progression through the miR-195-5p/MAPK signaling pathway, providing new hope for CC diagnosis and treatment.
Atelectasis, Shunt, and Worsening Oxygenation Following Reduction of Respiratory Rate in Healthy Pigs Undergoing ECMO: An Experimental Lung Imaging Study
Authors: Spinelli Elena , Colussi Giulia , Dal Santo Gaia , Scotti Eleonora , Marongiu Ines , Garbelli Erica , Mazzucco Alessandra , Dondossola Daniele , Maia Raquel , Battistin Michele , Biancolilli Osvaldo , Rosso Lorenzo , Gatti Stefano , Mauri Tommaso 
Published: April 2021

Rationale: Reducing the respiratory rate during extracorporeal membrane oxygenation (ECMO) decreases the mechanical power, but it might induce alveolar de-recruitment. Dissecting de-recruitment due to lung edema vs. the fraction due to hypoventilation may be challenging in injured lungs.Objectives: We characterized changes in lung physiology (primary endpoint: development of atelectasis) associated with progressive reduction of the respiratory rate in healthy animals on ECMO.Methods: Six female pigs underwent general anesthesia and volume control ventilation (Baseline: PEEP 5 cmH2O, Vt 10 ml/kg, I:E = 1:2, FiO2 0.5, rate 24 bpm). Veno-venous ECMO was started and respiratory rate was progressively reduced to 18, 12, and 6 breaths per minute (6-h steps), while all other settings remained unchanged. ECMO blood flow was kept constant while gas flow was increased to maintain stable PaCO2.Measurements and Main Results: At Baseline (without ECMO) and toward the end of each step, data from quantitative CT scan, electrical impedance tomography, and gas exchange were collected. Increasing ECMO gas flow while lowering the respiratory rate was associated with an increase in the fraction of non-aerated tissue (i.e., atelectasis) and with a decrease of tidal ventilation reaching the gravitationally dependent lung regions (p = 0.009 and p = 0.018). Intrapulmonary shunt increased (p &lt; 0.001) and arterial PaO2 decreased (p &lt; 0.001) at lower rates. The fraction of non-aerated lung was correlated with longer expiratory time spent at zero flow (r = 0.555, p = 0.011).Conclusions: Progressive decrease of respiratory rate coupled with increasing CO2 removal in mechanically ventilated healthy pigs is associated with development of lung atelectasis, higher shunt, and poorer oxygenation.