bluuebunny/crossref_metadata_2025_split huggingface dataset:
What You Don’t Know Might Hurt You: Cost Variations for Fecal Immunochemical Tests in Primary Care Practices
Authors: Coury Jennifer , Ramsey Katrina , Gunn Rose , Judkins Jon , Davis Melinda 
Published: March 2021

Abstract
Background

Colorectal cancer (CRC) screening can improve health outcomes, but screening rates remain low across the US. Fecal immunochemical testing (FIT) is an effective way to screen more people for colorectal cancer, but barriers exist to implementation in clinical practice. Little research examines the impacts of cost on FIT selection and implemention.
Methods

We administered a multi-modal, cross-sectional survey to 252 primary care practices to assess readiness and implementation of direct mail fecal testing programs, including the cost and types of FIT used. We analyzed the range of costs for the tests, and identified practice and test procurement factors. We examined the distributions of practice characteristics for FIT use and costs answers using the non-parametric Wilcoxon rank-sum test. We used Pearson's chi-squared test of association and interpreted a low p-value (e.g. <0.05) as evidence of association between a given practice characteristic and knowing the cost of FIT or fecal occult blood test (FOBT).
Results

Among the 84 practice survey responses, more than 10 different types of FIT/FOBTs were in use; 76% of practices used one of the five most common FIT types. Only 40 practices (48%) provided information on the cost of their FIT/FOBTs. Thirteen (32%) of these practices received the tests for free while 27 (68%) paid for their tests; median reported cost of a FIT was $3.04, with a range from $0.83 to $6.41 per test. Costs were not statistically significant different by FIT type. However, practices who received FITs from vendors were more likely to know the cost (p = 0.0002) and, if known, report a higher cost (p = 0.0002).
Conclusions

Our findings indicate that most practices without lab or health system supplied FITs are spending more to procure tests. Cost of FIT may impact the willingness of practices to distribute FITs in clinic-based encounters as well as through population outreach strategies, such as mailed FIT. Differences in the ability to obtain FIT tests in a cost-effective manner could have far reaching consequences for addressing colorectal cancer screening disparities in primary care practices.
Histologic findings of the dura mater at the level of the foramen magnum in 121 CKCS with Chiari-like malformation
Authors: Holdsworth Jaclyn P. , Marino Dominic J. , Loughin Catherine A. , Miller Andrew D. , Sackman Joseph J. , Lesser Martin L , O’Donnell Marissa 
Published: March 2021

Abstract
Background: To describe histopathologic features found in dural biopsies of Cavalier King Charles Spaniels (CKCS) with Chiari-like malformation (CM) and identify any associations between age, duration of clinical signs, syrinx location or syringomyelia (SM) and quality of life (QOL). The medical records of 121 consecutive client owned CKCS with CM and SM, confirmed by whole body magnetic resonance imaging (MRI), that underwent foramen magnum decompression (FMD) with cranioplasty and durectomy with biopsy from 2006 to 2016 were retrospectively reviewed. Dura biopsies were submitted to a board-certified veterinary pathologist for histopathologic interpretation. The chi-square test was used to analyze associations between diagnosis and categorical variables. For continuous measures, the Kruskal-Wallis non-parametric test was used to compare distributions across pathology categories. A result was considered statistically significant at the P<0.05 level of significance. Results: The mean age, duration of pre-surgical clinical signs, and pre-operative QOL (1-5 scale) were 44.27 months, 44.78 weeks, and 2.72 respectively. Syringomyelia was found in the cervical region only in thirty-nice of one hundred twenty-one (32.23%) of dogs, in the cervical and thoracic region only in seventeen of one hundred twenty-one (14.05%) of dogs, and in the cervical, thoracic and lumbar region combined in sixty-five of one hundred twenty-one (53.72%) of dogs. Sixty-six of one hundred twenty-one (54.55%) dural biopsy specimens had histopathology changes; fifty-five (45.45%) did not. Forty-three of one hundred twenty-one (35.54%) dural biopsy specimens had osseous metaplasia, sixteen of one hundred twenty-one (13.22%) had evidence of fibrosis, four of one hundred twenty-one (3.31%) had arachnoid hyperplasia, and three of one hundred twenty-one (2.48%) had evidence of mineralization. Conclusions: The majority of dogs with CM were found to have histopathologic changes in the dura at the time of FMD cranioplasty was performed. These dural changes can be observed in dogs experiencing clinical signs for a time period as short as four weeks prior to presentation. Histopathologic changes were not associated with age, breed, duration of clinical signs, the location of syringomyelia or preoperative QOL. The influence of histopathologic changes on long term prognosis in dogs without dural decompression is unknown since all dogs in this study had dural resection.
An asymmetric mode-localized mass sensor based on the electrostatic coupling of different structural modes with distributed electrodes
Authors: Song Jiahao , Lyu Ming , Kacem Najib , Zhao Jian , Liu Pengbo , Huang Yu , Fan Kefeng 
Published: March 2021

Abstract
Mode-localization sensor with amplitude ratio as output metric has shown excellent potential in the field of micro-mass detection. In this paper, an asymmetric mode -localized mass sensor with a pair of electrostatically coupled resonators of different thickness is proposed. Partially distributed electrodes are introduced to ensure the asymmetric mode coupling of second and third order modes while actuating the thinner resonator by the distributed electrode. The analytical dynamic model is established by Euler–Bernoulli theory and solved by harmonic balance method (HBM) combined with asymptotic numerical method (ANM). Detailed investigations on the linear and nonlinear behavior, critical amplitude as well as the sensitivity of the sensor are performed. The sensitivity of the proposed sensor can be enhanced by about 20 times compared to first order mode-localized mass sensors. Furthermore, by exploiting the nonlinearities while driving the device beyond the critical amplitude for the in-phase mode, the sensor performs a great improvement in sensitivity up to 1.78 times. Besides, the influence of the decrease of coupling voltage is studied, which gives a good reference to avoid mode aliasing.
Sub-second multi-channel magnetic control of select neural circuits in behaving flies
Authors: Sebesta Charles , Torres Daniel , Wang Boshuo , Li Zhongxi , Duret Guillaume , Jiang Kaiyi , Xiao Zhen , Zhang Qingbo , Colvin Vicki , Goetz Stefan , Peterchev Angel , Dierick Herman , Bao Gang , Robinson Jacob 
Published: March 2021

Abstract
Precisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or “magnetogenetics” using magnetic nanoparticle heating of temperature-sensitive ion channels enables remote, non-invasive activation of neurons for deep-tissue applications and studies of freely behaving animals. However, the in vivo response time of thermal magnetogenetics is currently tens of seconds, which prevents the precise temporal modulation of neural activity similar to light-based optogenetics. Moreover, magnetogenetics has not provided a means to selectively activate multiple channels to drive behavior. Here we demonstrate that by combining magnetic nanoparticles with a rate-sensitive thermoreceptor (TRPA1-A) it is possible to achieve sub-second behavioral responses in Drosophila melanogaster. Furthermore, by tuning the properties of magnetic nanoparticles to respond to different magnetic field strengths and frequencies, we can achieve fast, multi-channel stimulation, analogous to optogenetic stimulation with different wavelengths of light. These results bring magnetogenetics closer to the temporal resolution and multiplexed stimulation possible with optogenetics while maintaining the minimal invasiveness and deep-tissue stimulation only possible by magnetic control.
Dabigatran Treatment Increased Closure Device-Related Thrombosis by Platelet Activation in Patients Undergoing Percutaneous Left Atrial Appendage Closure
Authors: Li Xiaoye , Zhang Xiaochun , Jin Qinchun , Li Yanli , Ge Junbo , Zhou Daxin , lv qianzhou 
Published: March 2021

Abstract
Background

This study was designed to evaluate the platelet reactivity of different antithrombotic regimens under the condition of occluder implantation.
Methods

A single, prospective cohort study was conducted among patients who received anticoagulation with either dabigatran (N = 33) or rivaroxaban (N = 72) between January 2018 and December 2019. We applied thromboelastogram (TEG) to evaluate platelet aggregation induced with thrombin receptor activating peptide (TRAP) after anticoagulation for 3 months. Plasma coagulation markers mediate platelet activation including TAT, P-selectin, vWF and CD40L were tested by the method of ELISA kit on the day of LAAC and at 3 months after operation procedure. Repeated transesophageal echocardiographic were scheduled to evaluate device related thrombosis (DRT) formation on occluders at 3-month after discharge.
Results

There was 3(4.2%) in rivaroxaban and 4(12.1%) in dabigatran group experiencing DRT events (OR = 0.315, 95%CI:0.066–1.489, P = 0.129) during follow-ups. The TRAP induced platelet aggregation was higher for patients medication with dabigatran as compared to rivaroxaban group (62.9% vs. 59.7%, P = 0.028*). The plasma levels of TAT, P-selectin, vWF expression was significant higher after 3 months intake of dabigatran compared with that on the day LAAC operation, meanwhile, no significant difference was found in the changes of CD40L plasma levels. After receiving 3 months anticoagulation with rivaroxaban, the expressions of plasma platelet activation of TAT, P-selectin, vWF and CD40L showed no significant changes. We observed significant higher expressions of plasma platelet activation markers for DTR patients in terms of the P-selectin and vWF compared with non-DRT patients. Multivariate regression shwed that anticoagualtion regimen (P = 0.022; OR = 4.366, 95%CI: 0.434–10.839) was an independent predictor for DRT in patients after LAAC operation, while non of the plasma platelet activation included was associated with DRT.
Conclusions

By avoiding peri-procedure DRT occurrence, it is possible that dabigatran usage might even be reduced, as they had been shown to increase expressions of platelet reactivity.
Protection From Distributed Denial of Service Attack Using Fuzzy Logic
Authors: S Hemalatha , N Vasantha Gowri , A vani , Qaiyum Sana , P vijayakumar , Haleem Sulaima Lebbe Abdul 
Published: March 2021

Abstract
Distributed Denial of Service (DDoS) attacks represent an important challenge for public cloud as they invade the offender and completely delete Cloud service in order to serve the correct user and at the same time against the targets that cause system and service lack of access on infected devices. DDoS (Distributed Denial of Service) attacks are usually specific efforts to drain the resources of the victim or interrupt connections to networks by legitimate users. Traditional internet infrastructure is susceptible to DDoS assaults, and through leveraging their flaws to set up assault networks or Botnets, it offers an opening for an intruder to reach a wide number of infected machines. In order to identify and sustain improved detection accuracy, this work focuses on evaluating the different works and recommending a better solution to accommodate the cloud environment. A fuzzy logic for the detection and safety of DDOS attacks is proposed in this paper. The Fuzzy logic is used to dynamically select an algorithm from a collection of defined supervised learning that distinguish various DDoS variations and ultimately choose the relevant traffic algorithm.
Electrospun Fibrous Sponge via Short Fiber for Mimicking 3D ECM
Authors: Li Yan , Wang Juan , Qian Dejian , Chen Liang , Mo Xiumei , Wang Lei , Wang Yan , Cui Wenguo 
Published: March 2021

Abstract
BackgroundMost of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous micro/nanofibers. Herein, 3D electrospun micro/nanofibrous sponge was fabricated via electrospinning, homogenization, short fiber and thermal crosslinking for 3D tissue regeneration of cells and vascular. ResultsFibrous sponge exhibited high porosity, water absorption and compression resilience and no chemical crosslinked agent was used in preparation process. In vitro studies showed that the 3D short fiber sponge provided an oxygen-rich environment for cell growth, which was conducive to the 3D proliferation and growth of HUVECs, stimulated the expression of VEGF, and well promoted the vascularization of HUVECs. In vivo studies showed that the 3D short fiber sponges had a good 3D adhesion to the chronic wound of diabetes in rats. Furthermore, 3D short fibrous sponges were better than 2D micro/nanofiber membranes in promoting the repair of diabetic full-thickness skin defects including wound healing, hair follicle regeneration, angiogenesis, collagen secretion. ConclusionTherefore, electrospun short fibrous sponges are special candidates for mimicking the 3D ECM and promoting 3D regeneration of tissue.
Ethnobotanical Survey of Medicinal Herbs in the Western Region in Syria (Latakia and Tartus)
Authors: Khatib Chadi , Nattouf Abdulhakim , Agha Mohamad Isam Hasan 
Published: March 2021

Abstract
ContextThe use of traditional Arabic medicine (TAM) has spread to treat various diseases in Syria since ancient time. They are cost-effective with fewer side effects and are more suitable for long-term use compared with chemically synthesized medicines.ObjectiveWe conducted ethnobotanical and ethno-medicine research on plants traditionally used to treat various diseases in Western region of Syria. MethodsField surveys were conducted during July 2019-December 2020 to document ethnobotanical information through oral interviews and designed semi-structured questionnaire from local herbalists (Tabib Arabi) and the elderly people and others who were familiar with traditional uses of plants particularly for medicinal. The queries were repeatedly made to increase the reliability of the data, during the field survey of 475 local inhabitants of 70 villages. ResultsIn this research we listed 258 medicinal plants belonging to 83 families in alphabetical order with the parts used and the method of preparation according to their therapeutic use. Of these studied plants, 185 are used to treat digestive disorders, 118 for respiratory diseases, 91 for several skin diseases, 87 for kidney and urinary tract disorders, 78 for diabetes, 18 for nervous system disorders, and a few plants for treatment the other diseases which are commonly spread in study area. The calculated results of medicinal use-value (MUV) showed that Olea europaea L., Origanum syriacum L. was ranked first (MUV= 0.934), while the lowest value was found for Fumana thymifolia (L.) Spach and Cistus creticus L. (MUV= 0.003).ConclusionA large portion of the uses of medicinal plants mentioned in the research are still under study. There is no doubt that its study will provide many new data that could contribute to further pharmacological discoveries by identifying the active ingredients and their mechanism of effect by doing a lot of pharmacological work to confirm the alleged biological activities of these plants.
PAX5 Haploinsufficiency Induces Immune Inhibitory-Related Molecules Expressions of CD8 + T Cells in the Tumor Microenvironment
Authors: liang mi , gong duanhao , wang lei , liang xue , meng jiao , huang wei , zhou jianfeng 
Published: March 2021

Abstract
Loss of function of PAX5 plays an important role in PAX5 mutation tumor, and PAX5 haploinsufficiency promoting tumorigenesis is related to immune escape. But the mechanisms of PAX5 mutations inducing tumor immune escape have not been clarified. We estimated the proportions of 22 immune cell types and the expressions of immune inhibitory-related molecules based on gene expression profiles (GEPs) from B- acute lymphoblastic leukemia(B-ALL) with PAX5 mutations by CIBERSORT, an established algorithm. We constructed the PAX5 haplodeletion A20 cell lines, built allografted A20 tumor models and evaluated the effect of PAX5 haplodeletion on immune inhibitory-related molecules in the tumor microenvironment (TME). Our results indicated the percentages of T cells in bone marrow of B-ALL with PAX5 mutations were not statistically different from that in bone marrow of B-ALL without PAX5 mutations, except for T follicular helper (Tfh) cells. But a variety of up-regulated immune inhibitory-related molecules in bone marrow of B- ALL with PAX5 mutations were identified. By different approaches, we found that several immune inhibitory-related molecules of CD8+ T cells in TME of PAX5 haplodeletion clones such as TIM3, NR4A1 and BATF, were increased significantly compared with that of PAX5 wild type control. The IFN-ɤ of CD8+ T cells in TME of PAX5 haplodeletion tumors was decreased significantly compared with that of PAX5 wild type control. Our study showed that PAX5 haploinsufficiency induced high expressions of TIM3, NR4A1 and BATF in the TME and was involved in CD8+ T cells dysfunction or exhaustion.
Carbapenem-Resistant Klebsiella Pneumoniae in Southwest China: Molecular Characterizations and Risk Factors Caused by NDM and KPC Producers
Authors: Li Zhaoyinqian , Ding Zixuan , Yang Jia , Liu Yao , Jin Xinrui , Xie Jingling , Li Tingting , Ding Yinhuan , Zeng Zhangrui , Liu jinbo 
Published: March 2021

Abstract
Background: Klebsiella pneumoniae is one of the most common Enterobacteriaceae. In recent years, carbapenem-resistant Klebsiella pneumoniae (CRKP) has become one of the most important carbapenem-resistant Enterobacteriaceae. CRKP are usually resistant to antibiotics. Up to this day, the emergence of carbapenemase-producing K. pneumoniae has been a challenge for treatment of clinical infection.Methods: (i) 66 non-repetitive clinical CRKP isolates were identified by matrix-assisted laser analytical ionization time-of-flight mass spectrometer (MALDI-TOF-MS) and drug sensitivity analysis was performed by Vitek2 Compact. EDTA-synergy test and mCIM / eCIM test were used to detect drug-resistant phenotypes. (ii) Carbapenemase genes, extended-spectrum β-lactamase genes (ESBLs), cephalosporinase gene (AmpC), virulence genes, integron and resistance gene cassettes were amplified by PCR. (iii) Plasmid typing was performed by plasmid conjugation assay and PCR-based replicon typing (PBRT) method. (iv) The genetic environments of KPC-2 and NDM-1 were analyzed by using overlapping PCR. (v) MLST was used to analyze the molecular epidemiological characteristics of CRKP. (vi) Risk factors of CRKP infection by logistic regression model.Results: Our study revealed that 42 of the 66 CRKP isolates obtained from patients were identified as blaKPC-2, 24 blaNDM-1-positive strains were identified (20 blaNDM-1 and 4 blaNDM-5), of which 18 were from the neonatal departments. And CRKP strains were ESBL (extended-spectrum β-lactamases) and AmpC enzymes producer, Notably, we found two CR-hvKp (carbapenem-resistant hypervirulent klebsiella pneumoniae) strains, which contains blaKPC-2 gene and other resistant genes. Two of the 42 KPC-2-producing CRKP strains were positive for transconjugants, and the plasmid typing was the IncFII type. And two NDM-producing CRKP strains tested positive for transconjugants, which belonged to the lncX3 plasmid. Analysis of the genetic environment of these two genes has revealed that the highly conserved regions (tnpA-tnpR-ISkpn8-blaKPC-2) and conserved regions (blaNDM−1-bleMBL-trpF-tat) are associated with the dissemination of KPC-2 and NDM-1. Intl1 carrying drug resistance gene cassettes were widely distributed in CRKP. According to the MLST results, a total of 13 ST types were measured in 66 CRKP strains, ST11 and ST4495 were the main ST types, and the latter was the newly discovered ST type. Hematological disease, tracheal cannula and prior use of β-lactams and β-lactamase inhibitor combination were identified as independent risk factors for CRKP infections.Conclusion: These findings manifested the need for intensive surveillance and precautions to monitor the further spread of KPC and NDM in southwest China.
GDF11 alleviates pathological myocardial remodeling in diabetic cardiomyopathy through SIRT1-dependent regulation of oxidative stress and apoptosis
Authors: Zhu Hanzhao , Zhang Liyun , Zhai Mengen , Xia Lin , Cao Yu , Xu Lu , Li Kaifeng , Jiang Liqing , Shi Heng , Li Xiang , Zhou Yenong , Ding Wei , Wang Dongxu , Gao Erhe , Liu Jincheng , Yu Shiqiang , Duan Weixun 
Published: March 2021

Abstract
Background

Diabetic cardiomyopathy (DCM) is characterized by cardiac dysfunction and cardiomyocyte injury, which induced by metabolic disorder. Nowadays, there is still a lack of drugs for the treatment of DCM. Growth differentiation factor 11 (GDF11) is a novel member of the transforming growth factor β superfamily that alleviates cardiac hypertrophy, myocardial infarction, and vascular injury by regulating oxidative stress, inflammation, and cell survival. However, the roles and underlying mechanisms of GDF11 in DCM remain largely unknown.
Methods

In this study, we sought to determine whether GDF11 could prevent DCM. The mouse model of diabetes was established by administering a high-fat diet and intraperitoneal injecting streptozotocin. After that, intramyocardial injection of an adeno-associated virus carrying GDF11 gene was used to achieve myocardium-specific overexpression.
Results

Our data showed that GDF11 overexpression remarkably improved cardiac dysfunction and interstitial fibrosis by reducing the levels of reactive oxygen species and protecting against cardiomyocyte loss. Mechanistically, decreased sirtuin 1 (SIRT1) expression was observed in diabetic mice, which was significantly increased after GDF11 overexpression. To further explore how SIRT1 mediates the role of GDF11, the selective inhibitor EX527 was used to block SIRT1 signaling pathway, which abolished the protective effects of GDF11 against DCM. In vitro studies confirmed that GDF11 protected against H9c2 cell injury in high glucose and palmitate by attenuating oxidative injury and apoptosis, and these effects were also eliminated by SIRT1 depletion.
Conclusion

Our results demonstrated for the first time that GDF11 protected against DCM by regulating SIRT1 signaling pathway, and GDF11 had the potential to become a novel target for reversing cardiac dysfunction in diabetic patients.
A novel proposal for ultra-fast all optical half subtractor based on nonlinear ring resonators
Authors: Khajeheian Niloofar , Jamali Jasem , Fatehi-Dindarlou Mohammadhossein , Taghizadeh Mehdi 
Published: March 2021

Abstract
Optical half subtractors are required for designing any optical computation system. Operation speed and the on/off contrast ratios are very important characteristics for any optical logic device. A novel structure composed of three nonlinear ring resonators and some optical waveguides are used for designing for the proposed half subtractor with improved operation speed and on/off contrast ratio. The simulation results show that the rise time for this structure is 1.5 ps. Also the on/off contrast ratios for B and D are 13.7 dB and 14.5 dB respectively.