bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psittacidae community in Mbeliling landscape, Flores
Authors: Tumakaka J P , Winarni N L , Pradana D H , Junaid A R 
Published: January 2021

AbstractA study of Psittacidae community has been conducted in the area of Mbeliling landscape, Flores, East Nusa Tenggara. The purpose of this study was to asses the community ofPsittacidaewhich includes the relative abundance, dominance and species composition in three locations with their different representations of habitats and altitudes. Data were collected in May and July 2013 from three locations, namely Lengkong Ra’beng, Dencang Mese and Wae Ndae. The data collection method used is Point Count. As many as 330 individual Psittacidae were found which are included in 4 different species;Tanygnathus megalorynchos,Geoffroyus geoffroyi,Trichoglossus haematodus weberiandLoriculus flosculus. Amongst the four species found,Loriculus flosculusis listed as Flores’s endemic species andTrichoglossus haematodus weberiis listed as Flores’s endemic sub-species. The relative abundance of each species are expressed in encounter rates. The dominance index value of Mbeliling region is at 0.547. Results shows that these four species are dispersed in different group clusters in composition dependant to the type of habitats represented at three sampling sites. Results pointed out the importance of Mbeliling Landscape area for Psittacidae conservation in Flores.
Diversity of dragonflies and damselflies in lakes of Universitas Indonesia, Depok, West Java
Authors: Akbar L A , Basukriadi A 
Published: January 2021

Abstract
We investigated the diversity and distribution of Odonata inhabiting lakes at Universitas Indonesia, Depok, West Java in November 2017. The Study aimed to characterize the lakes based on the occurrence of some Odonata species. There were six study sites: Lake Agathis, Lake Kenanga, Lake Mahoni, Lake Puspa, Lake Salam, and Lake Ulin. Species of Odonata encountered within the study sites were caught using sweep nets, photographed and then released. A total of 16 species of Odonata were found including 12 dragonflies and 4 damselflies. The dragonflies were represented by two families. Of the 12 species of dragonflies recorded, 11 were from the family Libellulidae and 1 was from the family Gomphidae. Brachydiplax chalybea and Orthetrum testaceum (Fam. Libellulidae) were the most widely distributed species. However, the abundant species were O. testaceum and Zyxomma obtusum. Damselflies were poorly represented, with only four species from two families, Coenagrionidae and Platycnemididae. Based on the species composition of Odonata from six lakes at Universitas Indonesia we conclude that most species are eurytopic or generalist that are widespread and tolerant to a wide range of environmental variables. In the current study, we consider that all lakes at Universitas Indonesia are disturbed habitats which are generally unsuitable for specialist or stenotopic species.
Variations in the morphology of Hibiscus rosa-sinensis crested peach flowers in nature
Authors: Saifudin  , Salamah A 
Published: January 2021

AbstractHibiscus rosa-sinensishas a wide variety of flowers. The variations even can be observed in one hybrid only. The hybrid is known as crested flower (double type I), one of four categories ofH. rosa-sinensisflower shapes in nature. This study purpose was to categorize the variation in crested flowers based on morphological observation using 200 samples. The observations showed the form of crested peach could be divided into three groups: crested peach that resembles a single flower (crested single-like), which resembles a double flower (crested double-like), and a transitional between single-like and double-like flower (crested intermediate-like). Groupings are based on the length of the staminal column which measured at the closest distance between the corolla position and the additional stamen or petal. The length of the staminal column on acrested single-likeis more than 4 cm (a > 4 cm), whereas thecrested double-likeis less than 1 cm (a < 1 cm), and thecrested intermediate-likeis 1–4 cm (1 ≤ a ≤ 4). Groupings are also reinforced by comparing the average number of staminodium petaloid, stamen-petal intermediate, and stamen. Thecrested single-likehas a 1:2:2 ratio, thecrested intermediate-likehas a 2:1:1 ratio, while thecrested double-likedhas a 5:1:1 ratio. Of the total 200 crested samples used in the study, 89.5 % of them hadintermediate-likeforms, while 6.5 % weredouble-like, and 4 %single-like. It can be concluded that the real crested ofH. rosa-sinensisis anintermediate-likeform.
Abundance of microplastic in green mussel Perna viridis, water, and sediment in Kamal Muara, Jakarta Bay
Authors: Fathoniah I , Patria M P 
Published: January 2021

Abstract
Research on the abundance of microplastic in green mussel Perna viridis, water and sediments in Kamal Muara, North Jakarta in September 2017 has been done. The research determined the abundance of microplastic in water, sediment and green mussel (base on the sizes and microplastic storage organs), also the correlation between abundance of microplastic in green mussel, water and sediments. Samples of green mussel, water and sediments were taken from 3 different stations with a distance of about 500 meters. Analysis of abundance of microplastic was done by isolating microplastic in each sample. The isolation of the green mussel samples was done by dissolving the mussels in the HNO3 solutionfor 24 hours and added saturated NaCl solution. The microplastics from water and sediment samples were separated by immersion of samples in a saturated NaCl solution. The results obtained were, on average, abundance of microplastic in green mussel size 3, 6, and 9 cm i.e., 5.35; 24.99; and 39 particles/gram. The microplastic fiber was dominant in mussel sample. The average abundance of microplastic in water and sediment are 13.15 particles/L of sea water and 0.92 particles/gram of dry sediment respectively. The microplastic film was dominant in water and sediment samples. Meanwhile, pellet was not found in all three samples. There was a correlation between abundance of microplastic with green mussel size, as well as with abundance of microplastic of film and fiber in water and sediment.
Optimization of annealing temperature for detection of lipase gene in Bacillus subtilis using polymerase chain reaction (PCR) method
Authors: Sugiarti S A , Nurhayati N , Abinawanto  
Published: January 2021

Abstract
Enzyme lipase (EC 3.1.1.3, triacylglycerol acylhydrolase) is a group of enzymes that hydrolyzes fat into fatty acids and glycerol. In addition, the lipase enzyme plays a role in various reactions, such as esterification, interesterification, and transesterification reactions. Lipase can be obtained from microbes, plants, and animals. The most important source of lipase is microorganisms that are produced by fermentation of different bacteria. Microbial Lipases are easy to produce and cost of production is low. Bacteria like Bacillus are commercially used for lipase production. Recombinant lipase enzyme can be expressed by lipase gene (lip gene) and secreted in the bacterial Bacillus subtilis. Polymerase Chain Reaction (PCR) is in vitro amplification Deoxyribonucleic acid (DNA) method. The purity and yield of the reaction products depend on several parameters, one of which is the annealing temperature. The aim of this research was to optimize an annealing temperature for lip gene amplification using PCR. The success in amplifying a gene by PCR technique using a specially designed primer is determined by the precision of the primary attachment temperature with the DNA. Two Primer pairs are designed to successfully amplify DNA sequence fragment, namely xynlip_for_41_nt_and xynlip_rev_38_nt_. The primer combination produced the best results regarding band intensity and relative absence of unspecific bands, producing the expected amplicons of approximately 792 bp. Regarding the annealing temperatures, the best amplification was obtained at 60 °C.
Study of Heat Shock Protein (Hsp) 70 gene expression of Emilia sonchifolia L. and Sphagneticola trilobata L. in Universitas Indonesia, Depok and Kebun Raya Cibodas
Authors: Syadewi M , Salamah A , Maryanto A E , Andayani N 
Published: January 2021

Abstract
The temperature of earth surface is generally different according to altitude. Universitas Indonesia (UI) is located at 50–140 masl with an average temperature of 28.6 °C, while Kebun Raya Cibodas (KRC) located at 1.300–1.425 masl with an average temperature of 20.06 °C. Temperature differences are thought to affect plant responses, such as the expression of heat shock protein (Hsp) 70 genes. The research aims to find out the expression of Hsp70 genes on Emilia sonchifolia and Sphagneticola trilobata collected from UI and KRC. The study was conducted by isolating RNA from young leaves, which then converted into cDNA. The cDNA product was further amplified by polymerase chain reaction (PCR) using Hsp70 Arabidopsis thaliana primer. The amplification products were then sequenced and analyzed by in-silico. The results of amplification show that there is a band with approximately 250 bp in all samples which is thought to be a partial product of the Hsp70 gene. The sequencing results show that PCR amplification product is Hsp70 partial gene with a nucleotide variation in the 65th base which has no effect on amino acid changes. The results indicate that Hsp70 gene is expressed in Emilia sonchifolia and Sphagneticola trilobata grown in UI and KRC.
The effect of addition glutathione antioxidant on vitrification medium to the viability of Garut sheep (Ovis aries) oocytes
Authors: Gunawan M , Pratiwi P A , Kaiin E M , Sjahfirdi L 
Published: January 2021

Abstract
Research has been conducted to investigate the viability of Garut sheep oocyte to evaluate the effect of glutathione antioxidants addition with a concentration of 0.5 mM, 1 mM, and 1.5 mM in the vitrification medium to the viability of Garut sheep oocytes. The oocytes was freezed at -196 °C for 7 days. A total of 135 oocytes first were matured in vitro with the addition of 1 mM glutathione for 24 hours. The oocyte were vitrified using 15 % ethylene glycol, 15 % dimethyl sulfoxide, 0.5 M sucrose and divided into four treatments group with the addition of 0 mM (KK), 0.5 mM (KP 1), 1 mM (KP 2), and 1.5 mM (KP 3) glutathione. Viability was evaluated through the luminescence produced by Hoechst and Propidium Iodide dyes. The percentage of viable oocytes are 69.70 % (0 mM), 72.73 % (0.5 mM), 76.47 % (1 mM), and 77.14 % (1.5 mM). The results tends to increase along with the addition of glutathione concentration. However, statistically, ANOVA were not significantly different among treatment groups (P > 0.05). The conclusion of the study is that the addition of glutathione antioxidants in the vitrification medium are able to maintain the viability of Garut sheep oocytes during vitrification, although the ANOVA test were not significant.
Microplastic abundance in anchovy Stolephorus indicus (Van Hasselt, 1823) in the Lada Bay, Pandeglang, Banten
Authors: Hardianti E , Wardhana W , Patria M P 
Published: January 2021

Abstract
Currently, microplastic is one of the serious threats to the marine environment. This research aims to investigate the abundance and types of microplastics contained in anchovies Stolephorus indicus and water from Teluk Lada (Lada Bay), Tanjung Lesung, Pandeglang, Banten. Anchovy samples were extracted with 1M NaOH and 5 % sodium dodecyl sulphate (SDS). Meanwhile, water samples were treated by mixing it into NaCl concentrate solution. The results show that 246.10 ± 32.25 ind−1 and 156 ± 13.46 L−1 microplastic particles were found in the anchovies and water, respectively. In addition, 216.30 ± 30.13 ind−1 microplastics were found in the anchovies taken from traditional market as a control. The types of microplastics found in anchovies were fibers (77.41 %), films (16.64 %), fragments (5.6 %) and a little amount of granules (0.35 %). Meanwhile, the water samples were contained with fibers (81.03 %), films (11.92 %), fragments (7.0 %) and a little amount of granules (0.05 %). Our study found that fibers were the dominant pollutant in all samples and the amount of microplastics contained in water is strongly related to the amount of microplastics found in anchovies.
The effect of giving corncob (Zea mays) on the growth of sludge worm (Tubifex sp.)
Authors: Khaerunnisa S , Safitri S D , Ikhsan C , Purwanti D , Lestari R 
Published: January 2021

Abstract
One of the best feed choices for aquaculture is sludge worms (Tubifex sp.). Sludge worms need enough protein for worm growth. Corncob is a waste that is not utilized in the environment. Corncob has protein content that can be used for sludge worm growth. Complex carbohydrates are broken down through fermentation. The purpose of this study was to optimize the development of sludge worm cultivation media based on corncobs. Sludge worms are cultured in a series of culture containers by recirculating watering. There are 3 variations of treatment, namely, treatment-I (10 %), treatment-II (20 %) and treatment-III (30 %). The parameters measured are water quality, dissolve oxygen, water flow rate, mass, number of individuals in the population, total organic matter. The results prove using corncob as a medium enrichment material prov idegood environmental results and is suitable for sludge worm. The range of temperature, pH and DO values are at the optimal conditions needed for the best fermentation process.
Lignocellulolytic enzyme activity pattern of three white oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) strains during mycelial growth and fruiting body development
Authors: Nurfitri N , Mangunwardoyo W , Saskiawan I 
Published: January 2021

Abstract

Pleurotus ostreatus is one of the edible mushrooms that can utilize lignocellulose as substrate because of their ability to secrete the lignocellulolytic enzyme. The purpose of this research is to investigate and compare the lignocellulolytic enzymes production of P. ostreatus InaCC F209, F216 and LIPI on solid-state fermentation using sawdust during 70 days of mycelial growth (vegetative phase) and fruiting body development (reproductive phase). Supernatant of the extracted enzyme solutions were employed to investigate the reducing sugar, soluble protein, and enzyme activities. The results revealed that reducing sugar concentration of the three P. ostreatus strains subjected increased during growth and reached the maximum concentration on the reproductive phase, while the total protein content fluctuated during the growth but reached the maximum concentration on the reproductive phase. Laccase, LiP, and MnP activities of three P. ostreatus strains were higher on the vegetative phase, while the endoxylanase and endoglucanase activities were higher on the reproductive phase. β-glucosidase activity showed different variations between three P. ostreatus strains. Pleurotus ostreatus InaCC F209 produced the highest and most stable laccase, β-glucosidase, endoglucanase, and endoxylanase than two others.
fshr and lhr mRNA expression in granulosa cells of poor responder
Authors: Hanifah N , Wiweko B , Bowolaksono A 
Published: January 2021

Abstract
In Vitro Fertilization (IVF) is one of the most commonly used procedures to help pregnancies in couples who have infertility problems. One of the problems of infertility is poor ovarian response and the woman who experiences it is known as poor responder. Poor responders do not have an adequate ovarian response to gonadotropin in ovarian stimulation. The success of fertilization in poor responders tends to be low due to low quantity and is generally followed by low oocyte quality. Gonadotropins consisting of FSH and LH, play a role in follicle development and ovulation. The follicle response in capturing gonadotropins depends on the exact bond between the hormone and its receptor (FSHR and LHR) in the granulosa cells surrounding the oocyte. The purpose of this research is to know the expression level of fshr and lhr mRNA in granulosa cells of poor responders through real-time PCR method which then tested statistically using t-test. Fourteen samples of each poor responders and normal women were used in this research. The results showed insignificant differences between expression level of fshr and lhr mRNA in granulosa cells of poor responders and normal women (p > 0.05).
Production of antimicrobial compound from two potential actinomycetes SRM 2 and SD 17 using soluble and local starch
Authors: Prasetiyo H D , Santoso I , Maryanto A E 
Published: January 2021

Abstract
Fermentation of antimicrobial compound from two potential actinomycetes, SRM 2 and SD 17, has been carried out using a local carbon source and commercial soluble starch. Three local carbon sources, rice, corn, and tapioca starch were screened to determine the best local carbon source for growth medium of actinomycetes. Determination was based on Amylolytic Index Activity (AIA) using Iodine test. Result from screening local starch test of both actinomycetes SRM 2 dan SD 17 showed that rice starch was the best carbon source with AIA value (0.87 and 2.30) higher than corn starch (0.50 and 1.43) and tapioca starch (0.38 and 1.71). Antimicrobial compound of actinomycetes SRM 2 and SD 17 was produced in CSM medium containing soluble starch (CSMs) and rice starch (CSMb). Filtrate of the growth medium was tested for antimicrobial activity by using cylinder method against certain Gram positive and negative bacteria or yeasts. The results showed that filtrate of medium CSMs and CSMb from actinomycetes SRM 2 were most effective to inhibit Kocuria rhizophila with Antimicrobial Index (AI) 2.79 and 2.66, respectively. The filtrate was less effective against to Escherichia coli with AI 0.92 and 0.89. Filtrates of medium CSMs and CSMb from actinomycetes SD 17 were also most effective to inhibit K. rhizophila with AI 1.75 and 3.16 respectively. Meanwhile, the filtrates were less effective to inhibit Candida albicans with IA 0.67 for CSMs and 0.95 for CSMb, but not effective to inhibit Saccharomyces cerevisiae.
Pseudo 3D seismic using kriging interpolation
Authors: Abdullah F , Yulianto E , Novrizal  , Riyanto A 
Published: January 2021

Abstract
Seismic method is one of geophysical method that is used to determine subsurface condition of the earth. There are two types of seismic data, 2D seismic and 3D seismic. The advantages of using 3D seismic data is it has less uncertainty for imaging the earth subsurface. However, the cost to acquire 3D data is higher than producing 2D data, thus some company needs to invest more money to be able to get a better data quality. One of the ways to have 3D seismic data without having to spend a lot of money is by creating a 3D pseudo seismic data from 2D seismic data. The purpose of this research is to create a 3D pseudo seismic data by using a geostatistical method. The geostatistical method that is used in this research is Kriging interpolation. By the help of Petrel software, kriging interpolation is done to create the 3D seismic. The pseudo 3D seismic results show a good image such as difference in amplitude, also structural geology features of subsurface. In conclusion, the use of kriging interpolation in pseudo 3D seismic still needs more work and development so the results will be good for the next interpretation steps.
Evaluation study on the effect of production and fluid reinjection towards reservoir using micro gravity in Kamojang geothermal field
Authors: Dwikorianto T , Hendriansyah T , Sastranegara R M T , Nugroho S I 
Published: January 2021

Abstract
Geothermal fluid exploitation is expected to cause physical as well as chemical changes to the reservoir of a geothermal field. This is what happened to Kamojang Geothermal Field which has been producing for four periods, starting from the initial production capacity of 30 MW (1983) which became 140 MW (1987), then 200 MW (2008) and 235 MW since 2015 up to now. To observe changes of subsurface condition, Microgravity, is called time-lapse gravity as well, as one of geophysical survey activity is carried out in order to obtain the reservoir changes in a wider view based on the changes of gravity value as a reflection of rock density changes due to the extracted fluid mass. Generally, the microgravity study result from 1984 until 2018 shows the existence of microgravity value changes which correlates to the amount of fluid mass produced and the water mass which was reinjected back into the reservoir. This can be observed from the Time-lapse Anomaly Map in accordance to each of the production period of Geothermal Power Plant (GPP) Unit 1, 2, 3, 4 and 5.
Sandstone diagenesis interpretation of Cipacar formation based on outcrop data in Banjarsari District, Lebak Regency, Banten
Authors: Mahesa F G , Riyanto A , Indraswari A O 
Published: January 2021

Abstract
The diagenesis of sediments is profoundly impacted by porosity and permeability since these parameters control the flow of fluids through rocks that build up mineral authigenesis, cementation, and dissolution. Porosity in rock is available in sedimentary rock, particularly in sandstone in Cipacar formation located in Banjarsari, Lebak, Banten. This research aims to examine sandstone characteristics, porosity value, diagenesis process, and implication between porosity and diagenesis. The data of this study were obtained from surface outcrops and petrographic analysis. Results of this research show that sandstones in Cipacar formation have porosity values between 8–14 % and have undergone diagenesis processes such as compaction, cementation, and dissolution which are classified in the mesogenesis regime.
Processing of noise contaminated magnetotelluric data using digital filters based on MATLAB
Authors: Sulaiman F A , Ismail M L , Daud Y 
Published: January 2021

Abstract
Magnetotelluric (MT) method is known as an effective geophysical method for imaging the condition of the subsurface resistivity distribution, especially in the geothermal system. However, this method is very sensitive to noise. The noise can occur due to mechanical activity such as vibration and electrical activity generated by powerline electric current near the point of measurement. To eliminate this noise is used digital filter consisting of despike filter and notch filter. Despike filter is used to eliminate irregular spike noise, while the notch filter is used to remove regular powerline noise. Furthermore, D+ can be applied to enhance the impedance data trend. Both of filters and D+ feature are built and then arranged into MTFilter program. The result of data processing using MTFilter program shows the improvement of MT data quality. Moreover, the inversion processing results of MT field data shows the resistivity distribution of structure which is more reliable with the characteristics of the geothermal system.
Simulation of mortality immunization for life insurance companies in Indonesia using duration and convexity approach
Authors: Wibisana A , Novita M 
Published: January 2021

Abstract
Duration and convexity are two important factors in interest rate immunization. These two factors used to be very closely related to financial assets immunization towards change in interest rate, but some of the latest studies had applied the concept of duration and immunization in terms of mortality immunization. This paper examines 24 mortality immunization strategies that applied the duration and convexity concept in insurance portfolios which consist of life insurance and annuity products. The outcome of this study is the optimal proportion of the life insurance and annuity products for life insurance companies in Indonesia. Numeric simulations had been done using Indonesia Mortality Table 2011 (TMI-2011) to obtain the value and characteristics of the optimal proportion for two portfolios which is affected by several factors, namely the implemented strategy, mortality model, mortality model change type, age of policy holder, year of application, payment paying period, and term policies.