bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract 4910: Integrated Approach By Using Coronary Flow Velocity Reserve And Coronary Flow Velocity Patterns In Predicting Long-term Adverse Cardiac Events In Acute Myocardial Infarction
Authors: Yoon Myeong Ho , Tahk Seung-Jea , Lim Hong-Seok , Park Jin-Sun , Yang Hyeong-Mo , Choi Un-Jung , Hwang Jung-Won , Hong Hye-Jeon , Kang Soo-Jin , Choi Byoung-Joo , Choi So-Yeon , Hwang Gyo-Seung , Shin Joon-Han 
Published: July 2021

Background: The microvascular function was known to be an useful predictor of left ventricular functional changes and clinical outcomes in ST-segment elevation myocardial infarction (STEMI). We evaluated the usefulness of integrated approach by using coronary flow velocity reserve (CFR) and diastolic deceleration time (DDT) in the prediction of long-term major adverse cardiac events in STEMI.
Methods and Results: Using an intracoronary Doppler wire, CFR, DDT and hyperemic microvascular resistance index (MVRI) were evaluated in 202 patients with first STEMI received reperfusion therapy within 24 hours after onset of symptoms. Major adverse cardiac events were the composite of cardiac death, recurrent myocardial infarction, congestive heart failure and stroke during an average follow-up period of 60 ± 39 months. Follow-up echocardiography was performed at 12 ± 9 months. CFR, DDT and MVRI had significant correlations with left ventricular regional wall motion score index at follow-up echocardiography (r =−0.441, p<0.001; r = 0.413, p<0.001; r =−0.485, p<0.001, respectively). Using receiver-operating characteristics analysis, CFR ≤1.3 (sensitivity: 51%, specificity: 78%), DDT ≤577 ms (sensitivity: 72%, specificity: 62%) and MVRI >2.7 (sensitivity: 68%, specificity: 67%) were the best cutoff values in the prediction of occurring the adverse cardiac events. In patients with CFR ≤1.3, DDT ≤577 ms, cardiac events were occurred in 18 patients (40.0 %) of 45 patients, whereas cardiac events were occurred in 12 patients (20.3%) of 59 patients with CFR >1.3 and DDT ≤577 ms or CFR ≤1.3 and DDT >577 ms (p= 0.048), 9 patients (9.1%) of 99 patients with CFR>1.3 and DDT >577 ms (p<0.001). Ejection fraction at admission (p=0.009), MVRI (p =0.002) and DDT (p=0.023) were independent predictors in the prediction of long-term adverse cardiac outcomes during follow-up.
Conclusions: Integrated approach by using CFR and DDT was useful in the prediction of long-term adverse cardiac events. MVRI and DDT were strong independent predictors of long-term adverse cardiac events in STEMI patients.
Abstract 16055: Lipoprotein Subclasses, Size, and Statin-Associated Incident Diabetes: An Analysis From the JUPITER Trial
Authors: Dugani Sagar , Akinkuolie Akintunde O , Glynn Robert J , Ridker Paul M , Mora Samia 
Published: July 2021

Statins reduce CVD events, LDL cholesterol (LDL-C) and triglycerides, with an increased risk of diabetes. The underlying predictors of statin-associated diabetes are unclear. We evaluated lipoprotein subclass and size changes in response to rosuvastatin to identify predictors of diabetes on statin therapy Among 11,918 non-diabetic participants in JUPITER (NCT00239681), lipoprotein subclasses and size were quantified by NMR spectroscopy (LipoScience, NC) prior to and 1 year after randomization to placebo or rosuvastatin (total 370 incident diabetes). Cox regression models were adjusted for diabetes risk factors Compared to baseline, rosuvastatin lowered LDL-C and particles by lowering cholesterol-enriched large LDL (58%) and IDL (46%), with less relative lowering of cholesterol-poor small LDL (22%), resulting in smaller LDL size (1.5%). Rosuvastatin lowered (15%-20%) triglycerides, VLDL triglycerides, and VLDL particles by lowering large (15%), medium (7%), and small (27%) particles, and increasing VLDL size (3%) (all p<0.0001). Among statin-allocated individuals, after adjusting for typical risk factors, incident diabetes was inversely associated with baseline levels of LDL-C, HDL-C, large LDL particles, and LDL size, and positively associated with baseline triglycerides, non-HDL-C, ApoB, LDL particles, VLDL particles, VLDL triglycerides and size (Table). Similar associations were seen in on-treatment rosuvastatin and placebo groups In JUPITER, random allocation to rosuvastatin altered the lipoprotein subclass profile in a manner associated with the development of diabetes Adjusted Hazard Ratios (95% CI) and Risk of Incident Diabetes with Rosuvastatin
Baseline parameters HR per 1-SD p value
LDL-C .86 (0.76-0.98) .02
HDL-C .69 (0.54-0.87) .002
Triglycerides 1.62 (1.41-1.86) <.0001
Non-HDL-C 1.20 (1.04-1.39) .01
ApoB 1.35 (1.18-1.55) <.0001
Total LDL* 1.32 (1.15-1.51) <.0001
Large LDL* .79 (0.71-0.87) <.0001
Small LDL* 1.71 (1.40-2.08) <.0001
IDL* .97 (0.85-1.11) .69
LDL size .66 (0.58-0.75) <.0001
Total VLDL* 1.16 (1.00-1.34) .046
Large VLDL* 1.78 (1.51-2.10) <.0001
Medium VLDL* 1.35 (1.15-1.58) .0002
Small VLDL* .93 (0.82-1.06) .30
VLDL size 1.58 (1.39-1.80) <.0001
VLDL triglycerides 1.51 (1.31-1.73) <.0001
*particles
Abstract 20526: Computational Fluid Dynamics in Partial Mechanical Circulatory Support: Implications for Patient Care
Authors: Partovi Sasan , Karmonik Christoph , Rengier Fabian , Mueller-Eschner Matthias , Meredig Hagen , Kauczor Hans-Ulrich , Arif Rawa , Schmack Bastian , Karck Matthias , Ruhparwar Arjang 
Published: July 2021

Introduction:
Partial mechanical circulatory support (pMCS) is used for the therapy of heart failure. The CircuLite® Pump has been introduced clinically with its inflow cannula connected to the left atrium and the outflow cannula to the right subclavian artery. Aim of our study was to visualize and quantify flow patterns using computational fluid dynamics (CFD) in CT angiography (CTA).

Methods:
Two heart failure patients with pMCS were imaged with ECG-gated CTA and echocardiography. CFD was performed in 3D derived from CTA using flow boundary conditions measured with ultrasound. Flow was visualized using velocity vectors of blood flow. Average velocity was calculated at 10 time points during cardiac cycle in the aorta and the innominate. Wall shear stress (WSS) was visualized on the wall of the digital model.

Results:
Flow reversal was observed in mid-systole for both cases distal from the origin of the innominate artery in both cases due to asynchrony of the constant flow of the device with the pulsatile flow of the heart (fig.). Maximum velocity of this back flow was 0.39 m/s in case 1 and 0.2 m/s in case 2. Therefore, further distal in the innominate artery, a region of slow and stagnant flow with low WSS at the artery wall was observed which changed during cardiac cycle.

Conclusions:
CFD analysis revealed an asynchronous behavior in the inducted flow patterns during systole. Further design should allow for synchronization with the native heart function.

Figure: On top flow during systole for both cases (case 1 on left), below flow during diastole. WSS is shown in pseudo-color representation with red indicating high values. Flow is visualized by arrows. During systole, a region of low WSS (blue) exists in the innominate artery which is absent during systole indicating flow reversal at this location.
Bottom panel: Velocity in inferior-superior direction during cardiac cycle for both cases. Red lines demonstrates change of direction of flow in the innominate during systole.
Abstract 14384: Scar Homogeneization Ablation in Patients With Ischemic Cardiomyopathy: Comparison Between Remote Magnetic Navigation and Manual Ablation
Authors: Di Biase Luigi , Tung Roderick , Burkhardt J. David , Romero Jorge , Trivedi Chintan , Mohanty Sanghamitra , Gianni Carola , Santangeli Pasquale , Bai Rong , Horton Rodney , Sanchez Javier , Gallinghouse G. Joseph , Hranitzky Patrick , Al-Ahmad Amin , Lakkireddy Dhanujaya , Lakkireddy Dhanujaya , Reddy Madhu , Beheiry Salwa , Hongo Richard , Shivkumar Kalyanam , Natale Andrea 
Published: July 2021

Introduction:
Remote magnetic navigation (RMN) and ablation with the magnetic irrigated tip catheter has been reported as a feasible and safe technique for the treatment of ventricular arrhythmias (VA). We compared the procedural benefit and the outcomes of patients with ischemic cardiomyopathy (IC) undergoing VAs ablation with the RMN versus the manual approach.

Methods:
Consecutive pts with IC undergoing VAs ablation both with RMN and with manual ablation at different Institutions were enrolled. Substrate mapping and ablation technique with scar homogenization were utilized for ablation with the end point of elimination of all abnormal electrograms within and or around the scar area. Procedural data and outcomes were analyzed. Post-ablation pacing maneuvers and isoproterenol were used to verify Vas inducibility.

Results:
A total of 218 consecutive pts (85.3% male, 69.2±7.7 years, LVEF 30.6±8.2) with IC were included with 80 pts undergoing manual ablation while 138 pts underwent RMN ablation. Patients with a scar size < 60 cm2 at the 3D voltage mapping system were excluded. The mean scar size was 140 ± 61 cm2. VT was inducible in 83% pts with a cycle length of 352.1±70.7 msec. The density of the substrate map was higher in the RMN group when compared to the manual ablation group (553±118 vs 347±97, p<0.001). Acute ablation success was achieved in 217 (99.5%) of pts. The mean procedural duration and fluoroscopy time was 255.8±116.6 min and 34.8±22.9 min respectively. The mean mapping time was lower in the RMN group (80.3 ± 13.8 min) compared to the manual ablation group (96.1 ± 20.3 minutes, p < 0.001), while the radiofrequency time was lower in the manual ablation group(66.1 ± 27.3 min vs. 74.4 ± 20.7 min, p=0.02).

At 15.4±6.8 months follow-up the success rate in the RMN group was 81.2% (112) while in the manual ablation group 55 (69%) pts were recurrence free (p=0.037).
There was one pericardial tamponade which required pericardiocentesis in the manual ablation group.

Conclusion:
This study shows that VAs ablation using RMN in pts with IC and a scar size greater than 60 cm2 increases success rate at follow up when compared to manual ablation. The better outcome might be due to the higher amount of time dedicated to RF applications to achieve scar homogenization rather than mapping
Marshallsussmanite, NaCaMnSi3O8(OH), a new pectolite-group mineral providing insight into hydrogen bonding in pyroxenoids
Authors: Origlieri Marcus J. , Downs Robert T. , Hoffman Derek R. , Ducea Mihai N. , Post Jeffrey E. 
Published: July 2021

AbstractMarshallsussmanite (IMA2013-067) is a new pyroxenoid mineral from the Wessels mine, Kalahari Manganese Field, Northern Cape Province, South Africa. Marshallsussmanite has ideal formula NaCaMnSi3O8(OH) and triclinic P$\bar{1}$symmetry. Marshallsussmanite forms vitreous pink bladed crystals to 2.1 cm. The mineral shows perfect cleavage on both {100} and {001}. The chemical composition from electron microprobe (average of 20 analyses) and inductively coupled plasma mass spectrometer analysis (average of three analyses) is Li2O 0.43, Na2O 8.06, MgO 0.08, CaO 15.33, MnO 21.79, SiO251.71; totalling 97.40 wt.%. The empirical formula, normalized to 3 Si and assuming 1 H apfu is Li0.100Na0.906Ca0.953Mg0.007Mn1.071Si3O8(OH). Unit-cell parameters from single crystal X-ray diffraction area= 7.7854(4),b= 6.9374(4),c= 6.8516(3) Å, α = 90.683(3)°, β = 94.330(3)°, γ = 102.856(3)°,V= 359.59(3) Å3;Z= 2. The crystal structure refinement converged withRobs= 0.0248 and site occupancy refinement gives crystal chemistry [Na0.948Li0.052][Ca0.793Mn0.207] [Mn0.937Ca0.063]Si3O8(OH). Marshallsussmanite is a single chain silicate with a repeat interval of three tetrahedra (i.e.dreierchain). Marshallsussmanite is a member of the pectolite group of pyroxenoids, which also includes barrydawsonite-(Y), cascandite, pectolite, serandite and tanohataite. Parallel silicate chains form layers, intercalated with well-ordered cation layers. Calcium and Mn both exhibit octahedral coordination, while Na has four bonded interactions in a coordination sphere (radius 3 Å) of seven separate O atoms. Procrystal electron density and bond valence modelling results are compared. The mineral has an unusually strong hydrogen bond with O4⋅⋅⋅O3 separation of 2.458(2) Å. Unlike pectolite and serandite, O4 in marshallsussmanite acts as an H-bond donor and O3 is an H-bond acceptor. Cation ordering in pyroxenoids has a substantial impact on the H position and corresponding H-bonding schemata.
Performance of Web tools for predicting changes in protein stability caused by mutations
Authors: Marabotti Anna , Del Prete Eugenio , Scafuri Bernardina , Facchiano Angelo 
Published: July 2021

Abstract
Background
Despite decades on developing dedicated Web tools, it is still difficult to predict correctly the changes of the thermodynamic stability of proteins caused by mutations. Here, we assessed the reliability of five recently developed Web tools, in order to evaluate the progresses in the field.

Results
The results show that, although there are improvements in the field, the assessed predictors are still far from ideal. Prevailing problems include the bias towards destabilizing mutations, and, in general, the results are unreliable when the mutation causes a ΔΔG within the interval ± 0.5 kcal/mol. We found that using several predictors and combining their results into a consensus is a rough, but effective way to increase reliability of the predictions.

Conclusions
We suggest all developers to consider in their future tools the usage of balanced data sets for training of predictors, and all users to combine the results of multiple tools to increase the chances of having correct predictions about the effect of mutations on the thermodynamic stability of a protein.
Two homologous Salmonella serogroup C1-specific genes are required for flagellar motility and cell invasion
Authors: Zhou Xiujuan , Liu Bin , Liu Yanhong , Shi Chunlei , Fratamico Pina M. , Zhang Lida , Wang Dapeng , Zhang Jianhua , Cui Yan , Xu Ping , Shi Xianming 
Published: July 2021

Abstract
Background
Salmonella is a major bacterial pathogen associated with a large number of outbreaks of foodborne diseases. Many highly virulent serovars that cause human illness belong to Salmonella serogroup C1, and Salmonella ser. Choleraesuis is a prominent cause of invasive infections in Asia. Comparative genomic analysis in our previous study showed that two homologous genes, SC0368 and SC0595 in Salmonella ser. Choleraesuis were unique to serogroup C1. In this study, two single-deletion mutants (Δ0368 and Δ0595) and one double-deletion mutant (Δ0368Δ0595) were constructed based on the genome. All these mutants and the wild-type strain were subjected to RNA-Seq analysis to reveal functional relationships of the two serogroup C1-specific genes.

Results
Data from RNA-Seq indicated that deletion of SC0368 resulted in defects in motility through repression of σ28 in flagellar regulation Class 3. Consistent with RNA-Seq data, results from transmission electron microcopy (TEM) showed that flagella were not present in △0368 and △0368△0595 mutants resulting in both swimming and swarming defects. Interestingly, the growth rates of two non-motile mutants △0368 and △0368△0595 were significantly greater than the wild-type, which may be associated with up-regulation of genes encoding cytochromes, enhancing bacterial proliferation. Moreover, the △0595 mutant was significantly more invasive in Caco-2 cells as shown by bacterial enumeration assays, and the expression of lipopolysaccharide (LPS) core synthesis-related genes (rfaB, rfaI, rfaQ, rfaY, rfaK, rfaZ) was down-regulated only in the △0368△0595 mutant. In addition, this study also speculated that these two genes might be contributing to serotype conversion for Salmonella C1 serogroup based on their apparent roles in biosynthesis of LPS and the flagella.

Conclusion
A combination of biological and transcriptomic (RNA-Seq) analyses has shown that the SC0368 and SC0595 genes are involved in biosynthesis of flagella and complete LPS, as well as in bacterial growth and virulence. Such information will aid to revealing the role of these specific genes in bacterial physiology and evolution within the serogroup C1.
Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content
Authors: Chernova Alina I. , Gubaev Rim F. , Singh Anupam , Sherbina Katrina , Goryunova Svetlana V. , Martynova Elena U. , Goryunov Denis V. , Boldyrev Stepan V. , Vanyushkina Anna A. , Anikanov Nikolay A. , Stekolshchikova Elena A. , Yushina Ekaterina A. , Demurin Yakov N. , Mukhina Zhanna M. , Gavrilova Vera A. , Anisimova Irina N. , Karabitsina Yulia I. , Alpatieva Natalia V. , Chang Peter L. , Khaitovich Philipp , Mazin Pavel V. , Nuzhdin Sergey V. 
Published: July 2021

Abstract
Background
Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding.

Results
We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes for 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them.
Identified genomic regions included the loci involved in rare fatty acids variation on chromosomes 3 and 14, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil.

Conclusions
This is the first large scale implementation of high-throughput lipidomic profiling to sunflower germplasm characterization. This study contributes to the genetic characterization of Russian sunflower collections, which made a substantial contribution to the development of sunflower as the oilseed crop worldwide, and provides new insights into the genetic control of oil composition that can be implemented in future studies.
Transcriptomics unravels molecular players shaping dorsal lip hypertrophy in the vacuum cleaner cichlid, Gnathochromis permaxillaris
Authors: Lecaudey Laurène Alicia , Singh Pooja , Sturmbauer Christian , Duenser Anna , Gessl Wolfgang , Ahi Ehsan Pashay 
Published: July 2021

Abstract
Background
Teleosts display a spectacular diversity of craniofacial adaptations that often mediates ecological specializations. A considerable amount of research has revealed molecular players underlying skeletal craniofacial morphologies, but less is known about soft craniofacial phenotypes. Here we focus on an example of lip hypertrophy in the benthivorous Lake Tangnayika cichlid, Gnathochromis permaxillaris, considered to be a morphological adaptation to extract invertebrates out of the uppermost layer of mud bottom. We investigate the molecular and regulatory basis of lip hypertrophy in G. permaxillaris using a comparative transcriptomic approach.

Results
We identified a gene regulatory network involved in tissue overgrowth and cellular hypertrophy, potentially associated with the formation of a locally restricted hypertrophic lip in a teleost fish species. Of particular interest were the increased expression level of apoda and fhl2, as well as reduced expression of cyp1a, gimap8, lama5 and rasal3, in the hypertrophic lip region which have been implicated in lip formation in other vertebrates. Among the predicted upstream transcription factors, we found reduced expression of foxp1 in the hypertrophic lip region, which is known to act as repressor of cell growth and proliferation, and its function has been associated with hypertrophy of upper lip in human.

Conclusion
Our results provide a genetic foundation for future studies of molecular players shaping soft and exaggerated, but locally restricted, craniofacial morphological changes in fish and perhaps across vertebrates. In the future, we advocate integrating gene regulatory networks of various craniofacial phenotypes to understand how they collectively govern trophic and behavioural adaptations.
Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy
Authors: Ghaffari Sasan , Torabi-Rahvar Monireh , Aghayan Sajjad , Jabbarpour Zahra , Moradzadeh Kobra , Omidkhoda Azadeh , Ahmadbeigi Naser 
Published: July 2021

AbstractBackgroundThe successful ex vivo expansion of T-cells in great numbers is the cornerstone of adoptive cell therapy. We aimed to achieve the most optimal T-cell expansion condition by comparing the expansion of T-cells at various seeding densities, IL-2 concentrations, and bead-to-cell ratios. we first expanded the peripheral blood mononuclear cells (PBMCs) of a healthy donor at a range of 20 to 500 IU/mL IL-2 concentrations, 125 × 103to 1.5 × 106cell/mL, and 1:10 to 10:1 B:C (Bead-to-cell) ratios and compared the results. We then expanded the PBMC of three healthy donors using the optimized conditions and examined the growth kinetics. On day 28, CD3, CD4, and CD8 expression of the cell populations were analyzed by flow cytometry.ResultsT-cells of the first donor showed greater expansion results in IL-2 concentrations higher than 50 IU/mL compared to 20 IU/mL (P = 0.02). A seeding density of 250 × 103cell/mL was superior to higher or lower densities in expanding T-cells (P = 0.025). Also, we witnessed a direct correlation between the B:C ratio and T-cell expansion, in which, in 5:1 and 10:1 B:C ratios T-cell significantly expanded more than lower B:C ratios. The results of PBMC expansions of three healthy donors were similar in growth kinetics. In the optimized condition, 96–98% of the lymphocyte population expressed CD3. While the majority of these cells expressed CD8, the mean expression of CD4 in the donors was 19.3, 16.5, and 20.4%.ConclusionsOur methodology demonstrates an optimized culture condition for the production of large quantities of polyclonal T-cells, which could be useful for future clinical and research studies.
How to forget a “traumatic” experience: a case report of transient global amnesia after nasopharyngeal swab for Coronavirus disease 19
Authors: Ravaglia Sabrina , Zito Antonio , Ahmad Lara , Canavero Isabella 
Published: July 2021

Abstract
Background
Transient global amnesia (TGA) is a clinical syndrome characterized by a temporary short-term memory loss with inability to retain new memories, usually lasting 2 to 8 h. TGA may be related to several medical procedures, including angiography, general anesthesia, gastroscopy.

Case presentation
We report a 58-year-old woman who experiencing TGA one hour after the execution of her first-time nasopharyngeal swab for COVID-19. Brain MRI showed a typical punctate Diffusion Weight Image (DWI) hippocampal lesion.

Conclusions
This is the first report of TGA after the execution of nasopharyngeal swab for COVID-19. This association lengthen the list of medical procedures associated with TGA, and we discuss the possible plausible mechanisms by which a nasopharyngeal swab could trigger TGA.