bluuebunny/crossref_metadata_2025_split huggingface dataset:
Positive and negative control of helicase recruitment at a bacterial chromosome origin
Authors: Winterhalter Charles , Stevens Daniel , Fenyk Stepan , Pelliciari Simone , Marchand Elie , Cronin Nora B , Soultanas Panos , Costa Tiago R. D. , Ilangovan Aravindan , Murray Heath 
Published: August 2021

ABSTRACTThe mechanisms responsible for helicase loading during the initiation of chromosome replication in bacteria are unclear. Here we report both a positive and a negative mechanism for directing helicase recruitment in the model organism Bacillus subtilis. Systematic mutagenesis of the essential replication initiation gene dnaD and characterization of DnaD variants revealed protein interfaces required for interacting with the master initiator DnaA and with a specific single-stranded DNA (ssDNA) sequence located in the chromosome origin (DnaD Recognition Element, “DRE”). We propose that the location of the DRE within the replication origin orchestrates recruitment of helicase to achieve bidirectional DNA replication. We also report that the developmentally expressed repressor of DNA replication initiation, SirA, acts by blocking the interaction of DnaD with DnaA, thereby inhibiting helicase recruitment to the origin. These findings significantly advance our mechanistic understanding of helicase recruitment and regulation during bacterial DNA replication initiation. Because DnaD is essential for the viability of clinically relevant Gram-positive pathogens, DnaD is an attractive target for drug development.
Ribosome collisions in bacteria promote ribosome rescue by triggering mRNA cleavage by SmrB
Authors: Saito Kazuki , Kratzat Hanna , Campbell Annabelle , Buschauer Robert , Burroughs A. Maxwell , Berninghausen Otto , Aravind L. , Beckmann Roland , Green Rachel , Buskirk Allen R. 
Published: August 2021

AbstractRibosome rescue pathways recycle stalled ribosomes and target problematic mRNAs and aborted proteins for degradation. In bacteria, it remains unclear how rescue pathways distinguish ribosomes stalled in the middle of a transcript from actively translating ribosomes. In a genetic screen inE. coli, we discovered a novel rescue factor that has endonuclease activity. SmrB cleaves mRNAs upstream of stalled ribosomes, allowing the ribosome rescue factor tmRNA (which acts on truncated mRNAs) to rescue upstream ribosomes. SmrB is recruited by ribosome collisions; cryo-EM structures of collided disomes fromE. coliandB. subtilisreveal a distinct and conserved arrangement of the individual ribosomes and the composite SmrB binding site. These findings reveal the underlying mechanism by which ribosome collisions trigger ribosome rescue in bacteria.
Skin stem cells orchestrate de novo generation of extrathymic regulatory T cells to establish a temporary protective niche during wound healing
Authors: Truong Cynthia , Guo Weijie , Woodside Liberty , Gang Audrey , Savage Peter , Infarinato Nicole , Stewart Katherine , Polak Lisa , Levorse John , Pasolli Amalia , Dikiy Stanislav , Rudensky Alexander , Fuchs Elaine , Miao Yuxuan 
Published: August 2021

AbstractAdult stem cells reside in various tissues to govern homeostasis and repair damage. During wound healing, these stem cells must be mobilized to enter the center of the injury where they are exposed to many inflammatory immune cells infiltrating the wounded tissue. While these immune cells are indispensable for preventing infections and clearing dead cells, they can also create a harsh inflammatory environment which could potentially damage the stem cells and prevent their self-renewal and differentiation. Here, using a model of cutaneous wound healing in which hair follicle stem cells (HFSCs) repair the wound, we show that, upon migrating into the wound, skin stem cells acquire a strong immune modulatory capacity which allows them to sculpt a temporary immune suppressive niche for self-protection. We reveal that the HFSCs in the wound bed orchestrate extrathymic differentiation of regulatory T (Treg) cells by providing co-stimulation to the woundinfiltrating CD4 effector T cells. In this way, Treg cells can be generated de novo in close proximity to and can intimately protect HFSCs from the collateral damage inflicted by inflammatory neutrophils. This study uncovered a striking inflammatory adaptation capacity unique to adult tissue stem cells which allows them to shape their own immune suppressive niche during wound repair.
Inhibitors of anti-apoptotic Bcl-2 family proteins exhibit potent and broad-spectrum anti-mammarenavirus activity via cell cycle arrest at G0/G1 phase
Authors: Kim Yu-Jin , Witwit Haydar , Cubitt Beatrice , de la Torre Juan C. 
Published: August 2021

ABSTRACTTargeting host factors is a promising strategy to develop broad-spectrum antiviral drugs. Drugs targeting anti-apoptotic Bcl-2 family proteins that were originally developed as tumor suppressors have been reported to inhibit multiplication of different types of viruses. However, the mechanisms whereby Bcl-2 inhibitors exert their antiviral activity remain poorly understood. In this study, we have investigated the mechanisms by which obatoclax (OLX) and ABT-737 Bcl-2 inhibitors exhibited a potent antiviral activity against the mammarenavirus lymphocytic choriomeningitis virus (LCMV). OLX and ABT-737 potent anti-LCMV activity was not associated with their pro-apoptotic properties, but rather their ability of inducing cell arrest at G0/G1 phase. OLX and ABT-737 mediated inhibition of Bcl-2 correlated with reduced expression levels of thymidine kinase 1 (TK1), cyclin A2 (CCNA2), and cyclin B1 (CCNB1) cell cycle regulators. In addition, siRNA-mediated knock down of TK1, CCNA2, and CCNB1 resulted in reduced levels of LCMV multiplication. The antiviral activity exerted by Bcl-2 inhibitors correlated with reduced levels of viral RNA synthesis at early times of infection. Importantly, ABT-737 exhibited moderate efficacy in a mouse model of LCMV infection, and Bcl-2 inhibitors displayed broad-spectrum antiviral activities against different mammarenaviruses and SARS-CoV-2. Our results suggest that Bcl-2 inhibitors, actively being explored as anti-cancer therapeutics, might be repositioned as broad-spectrum antivirals.IMPORTANCEAnti-apoptotic Bcl-2 inhibitors have been shown to exert potent antiviral activities against various types of viruses via mechanisms that are currently poorly understood. This study has revealed that Bcl-2 inhibitors mediated cell cycle arrest at the G0/G1 phase, rather than their pro-apoptotic activity, plays a critical role in blocking mammarenavirus multiplication in cultured cells. In addition, we show that Bcl-2 inhibitor ABT-737 exhibited moderate anti-mammarenavirus activity in vivo, and that Bcl-2 inhibitors displayed broad-spectrum antiviral activities against different mammarenaviruses and SARS-CoV-2. Our results suggest that Bcl-2 inhibitors, actively being explored as anti-cancer therapeutics, might be repositioned as broad-spectrum antivirals.
On some nonlinear anisotropic elliptic equations in anisotropic Orlicz space
Authors: Benslimane Omar , Aberqi Ahmed , Bennouna Jaouad 
Published: August 2021

PurposeIn the present paper, the authors will discuss the solvability of a class of nonlinear anisotropic elliptic problems (P), with the presence of a lower-order term and a non-polynomial growth which does not satisfy any sign condition which is described by an N-uplet of N-functions satisfying the Δ2-condition, within the fulfilling of anisotropic Sobolev-Orlicz space. In addition, the resulting analysis requires the development of some new aspects of the theory in this field. The source term is merely integrable.Design/methodology/approachAn approximation procedure and some priori estimates are used to solve the problem.FindingsThe authors prove the existence of entropy solutions to unilateral problem in the framework of anisotropic Sobolev-Orlicz space with bounded domain. The resulting analysis requires the development of some new aspects of the theory in this field.Originality/valueTo the best of the authors’ knowledge, this is the first paper that investigates the existence of entropy solutions to unilateral problem in the framework of anisotropic Sobolev-Orlicz space with bounded domain.
Integrating interorganizational justice to facilitate tacit knowledge sharing in architectural and engineering design projects: a configurational approach
Authors: Ju Chuanjing , Ning Yan 
Published: August 2021

PurposeSharing tacit knowledge across firm boundaries is challenging in architectural and engineering design projects as tacit knowledge is embedded in the designer’s mind. It thus requires autonomous motivation. This study aims to examine how clients integrate distributive justice (DJ), procedural justice (PJ) and interactional justice (IJ) to motivate tacit knowledge sharing in interorganizational architectural and engineering (A/E) design projects.Design/methodology/approachA theoretical model was built, describing how the combination of distributive, procedural and interactional justice influences tacit knowledge sharing. This model was then verified using latent profile analysis (LPA) of 360 A/E design projects.FindingsA total offour subgroups with quantitatively different combinations of distributive, procedural and interactional justice were identified. Within each subgroup, the levels of the three forms of justice were quite aligned. The results indicate that clients often implement interorganizational justice in a collective manner. Among the four subgroups, projects with the highest level of justice combination have a significantly higher level of tacit knowledge sharing than the other three. This indicates that sharing of tacit knowledge is driven by the overall level of interorganizational justice.Originality/valueThe configurational approach complements previous research by presenting how combinations of interorganizational justice influence tacit knowledge sharing in A/E design projects.
Using health-care technology to manage COVID-19 pandemic: a case of Al-Ain city hospital, UAE
Authors: Al Ahbabi Jawaher Majdi , Ahmad Syed Zamberi 
Published: August 2021

Learning outcomes
The teaching objectives of the case study will enable the students as follows: to recognise the challenges of information technology (IT) implementation in the health-care sector associated with employee resistance, to apply the technology acceptance model for analysing the degree of employee resistance, to relate the utilisation of Kotter’s 8-step change management approach in successful IT implementation in the health-care sector and maintenance of employee productivity and to classify the leadership traits reflected by the leaders in training the 600 diverse employee population of Al-Ain hospital.

Case overview/synopsis
The case highlighted the predicament the government-owned Al-Ain City Hospital, United Arab Emirates, faced following the surge in the incidences of COVID-19 in the country in March 2020. The hospital management decided to initiate the work-from-home arrangement as a non-pharmaceutical intervention of handling the spread of the disease amongst its employees. Fatima Almur, the Information Technology Director in Al-Ain Hospital, asked the Application Support Manager, Aysha Shahwan, to deploy some IT tools significant for remote support to patient care within two weeks. Shahwan faced significant challenges in deploying the IT tools in two weeks given the diverse workforce, with the majority of them having limited knowledge in operating the tools, and hence, their apprehension in the usefulness of the tools. Besides, Shahwan had to deploy some advanced tools for easy and secured access to the electronic health record, telemedicine and telecommuting using mobile phones, tablets or PCs. The deployment of these advanced tools would be jeopardised by employee acceptance and consequent dwindling productivity. Considering the issue of employee acceptance of the change and their limited knowledge, Shahwan had, therefore, to develop training frameworks to boost the former’s perceived usefulness and ease-of-use of the IT tools. Will Shahwan successfully deploy the advanced IT tools to enable the hospital staff, including medical staff and departments, to ensure efficient patient care from a remote location? Will she be able to train the 600 employees across genders, ages and knowledge, use the IT tools and safeguard them from common software threats like email phishing and ransomware? Will the hospital be able to sustain its vision of quality patient care using advanced technologies through this new arrangement of remote support amidst the pandemic when patients are more?

Complexity academic level
Undergraduate business management.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 10: Public sector management.
Adaptability of piston skirt coatings on the tribological performance of heavy-duty diesel engine under low viscosity lubricant
Authors: Yin Bifeng , Wang Xuefeng , Xu Bo , Huang Gongyin , Kuang Xin 
Published: August 2021

Purpose
The purpose of this paper was to improve the frictional wear resistance properties of piston skirts caused by the low viscosity lubricant by studying the tribological performance of three novel coating materials.

Design/methodology/approach
Comparative tribological examinations were performed in a tribological tester using the ring-block arrangement under two viscosity lubricants, the loading force was applied as 100 N, the speed was set to 60 r/min and the testing time was 180 min.

Findings
Under low viscosity lubricant, the friction coefficient and wear of the three coatings all increase, and the friction coefficient and wear of the PTFE coating are the largest, while the MoS2 coating has the lowest friction coefficient and wear. Under low viscosity lubricant, the friction coefficient of the MoS2 coating is 2.1%–5.4% and 20.0%–24.3% lower than that of the SiO2 and PTFE coating, respectively. The friction coefficient and wear fluctuation rate of the MoS2 coating is the smallest when the lubricant viscosity decreases, which indicates that the MoS2 coating has excellent stability and adaptability under low viscosity lubricant.

Originality/value
To reduce the piston skirt wear caused by low viscosity lubricant in heavy-duty diesel engines, the friction and wear adaptability of three novel composite coating materials for piston skirts were compared under 0 W-20 low viscosity lubricant, which could provide a guidance for the application of wear-resistant materials for heavy-duty diesel engine piston skirt.
Simulation and experimental study on the influence of oil groove structure on the drag torque of the wet clutch
Authors: Wang Chengjun , Ding Wujian , Zheng Xudong , Zhu Haiqiang , Tian Zuzhi , Xie Fangwei , Gao Kuidong 
Published: August 2021

Purpose
This paper aims to design a single and double throat oil groove structure, which can reduce the drag torque of the wet clutch.

Design/methodology/approach
A three-dimensional simulation model was established herein using the computational fluid dynamics method. The influence of oil groove structure on the oil film flow field and the drag torque is obtained by a simulation.

Findings
Compared with the traditional radial oil groove, the results show that the single throat oil groove structure reduces the drag torque by about 24.59%; the double throat oil groove reduces the drag torque by about 47.27%. As the speed difference increases, the average temperature rise of the oil film of the double throat oil groove is 4°C lower than that of the single throat oil groove, indicating that it has good heat dissipation performance. The analysis results were verified by experimental results.

Originality/value
In this paper, the radial oil groove is taken as the reference object, and the structure of the oil groove is designed and improved. The simulation analysis and experiment verify the rule of the influence of the oil groove structure on the drag torque, which provides a new design idea for reducing the drag torque of wet clutch.
Dual-robot stud welding system for membrane wall
Authors: Chen Youdong , Hu Qi 
Published: August 2021

Purpose
The membrane wall is one of the most important components in the boiler industry and numerous studs are welded on its surface. The membrane wall welding still remains a sector intensive in the manual and arduous works. This paper aims to propose a dual-robot system to automatically weld studs on the membrane wall.

Design/methodology/approach
In this paper, the authors proposed a dual-robot stud welding system for membrane walls. First, the membrane wall is divided into several zones and the welding paths are planned. Then, the pose of the pipes is calculated based on the data measured by light section sensors. The planned paths are compensated by the pose. Finally, the robots weld studs based on the compensated paths.

Findings
The method effectively eliminates manufacturing errors and welding distortions. The system can weld straight type and L-type membrane walls with high efficiency, high quality and high accuracy.

Originality/value
The system can weld straight type and L-type membrane walls with high efficiency and high quality. Experiments were performed in a factory to demonstrate the practicability of the method. The dual-robot system with two welding machines has approximately twice the efficiency of the manual welder with only one welding machine. The quality and accuracy of robot welding systems are higher than that of manual welding.
Analyzing the impact of the COVID-19 pandemic on vegetable market supply in Northwestern Ethiopia
Authors: Worku Aemro , Ülkü M. Ali 
Published: August 2021

PurposeDue to global trade and transportation, the COVID-19 pandemic has rapidly reached all corners of the world; it has most impacted the poor communities and rural areas with limited or no access to recovery. This paper aims to understand the pandemic's impact on the market supply of vegetables (agrifood) in Ethiopia and other countries in sub-Saharan Africa and to implement scientifically based recommendations that can improve the challenges caused by the pandemic and improve the livelihoods of vegetable producers.Design/methodology/approachThis study assessed the major incidents that follow the occurrence of the COVID-19 pandemic in Ethiopia with the factors that significantly influence onion market supply in the Mecha district of Northwestern Ethiopia using linear multiple regression.FindingsThe study revealed that producers, processors, local collectors, cooperatives, wholesalers, retailers and consumers are the main actors. The most important difference that happened on the onion market channel after the outbreak of coronavirus was brokers were removed from the market channel after the pandemic and this saved the cost producers pay as a commission. The pandemic disrupted input distribution, extension and cooperative services and created labor scarcity. Access to market, postharvest value addition, price and marketing contract were significant factors that influence the market supply of onion. The study identified the need for policy interventions by the government to overcome the postpandemic challenges and ensure the sustainable development of onion production and marketing in the Mecha district.Research limitations/implicationsThe limitations of the study are primarily related to the methodology as data are collected at a single moment in time. However, the study observes that those changes after the pandemic are better understood if we collect data at different time. Therefore, the future study needs to provide longitudinal data to examine stability of response and to observe performance of the market that occur over time.Originality/valueThis original research is the first to study the impacts of the COVID-19 pandemic in Northwestern Ethiopia. The data used in the analysis are primary.
A set-theoretic approach to identifying effective configurations ofpromotional attributes
Authors: Qi Suntong , Peng Ling , Chen Yu-Jen 
Published: August 2021

PurposeMost previous studies have indicated promotional attributes separately (e.g. time pressure, giveaways, price discounts, exclusivity) and found controversial effects. This paper aims to explore how different attributes can be aligned with each other and integrated with different levels of brand strength to influence sales or purchase intention according to the fit logic.Design/methodology/approachBoth field data and controlled experiments are used to understand the effectiveness of promotion configurations. This paper first conducts an exploratory study using qualitative comparative analysis, based on 625 online promotion campaigns. This paper identifies several effective configurations of promotion attributes, leading to the development of two hypotheses. Three experiments are then designed to test the validity of these two hypotheses.FindingsThe results show that strong brands should adopt nonmonetary promotion, whereas weak brands should adopt monetary promotion; exclusivity and time limitation should be used separately.Research limitations/implicationsThis paper provides empirical insights on how promotion elicits sales in terms of the configuration of promotional attributes and brand strength. This paper examines the integrative effect of types of giveaways, promotion duration and promotion target as well as brand strength on promotion effectiveness. Future research can explore other promotional attributes. This study is a first attempt to introduce and apply the set-theoretic approach to address strategic research issues and inform strategic decisions and managerial actions.Practical implicationsThe findings can facilitate marketers’ understanding and predictions of deal recipients’ responses to promotions.Originality/valueThis paper adopts a holistic view to investigate how different promotion attributes interact with one another to stimulate sales. It explores the integrative effect from field data and finds converging evidence through a set-theoretic approach and controlled experiments.