bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon-induced transmembrane protein 3 (IFITM3) limits lethality of SARS-CoV-2 in mice
Authors: Zani Ashley , Kenney Adam D. , Kawahara Jeffrey , Eddy Adrian C. , Wang Xiao-Liang , KC Mahesh , Lu Mijia , Hemann Emily A. , Li Jianrong , Peeples Mark E. , Hall-Stoodley Luanne , Forero Adriana , Cai Chuanxi , Ma Jianjie , Yount Jacob S. 
Published: December 2021

ABSTRACTInterferon-induced transmembrane protein 3 (IFITM3) is a host antiviral protein that alters cell membranes to block fusion of viruses. Published reports have identified conflicting pro- and antiviral effects of IFITM3 on SARS-CoV-2 in cultured cells, and its impact on viral pathogenesisin vivoremains unclear. Here, we show that IFITM3 knockout (KO) mice infected with mouse-adapted SARS-CoV-2 experienced extreme weight loss and lethality, while wild type (WT) mice lost minimal weight and recovered. KO mice had higher lung viral titers and increases in lung inflammatory cytokine levels, CD45-positive immune cell infiltration, and histopathology, compared to WT mice. Mechanistically, we observed disseminated viral antigen staining throughout the lung tissue and pulmonary vasculature in KO mice, while staining was observed in confined regions in WT lungs. Global transcriptomic analysis of infected lungs identified upregulation of gene signatures associated with interferons, inflammation, and angiogenesis in KO versus WT animals, highlighting changes in lung gene expression programs that precede severe lung pathology and fatality. Corroborating the protective effect of IFITM3in vivo, K18-hACE2/IFITM3 KO mice infected with non-adapted SARS-CoV-2 showed enhanced, rapid weight loss and early death compared to control mice. Increased heart infection was observed in both mouse models in the absence of IFITM3, indicating that IFITM3 constrains extrapulmonary dissemination of SARS-CoV-2. Our results establish IFITM3 KO mice as a new animal model for studying severe SARS-CoV-2 infection of the lung and cardiovascular system, and overall demonstrate that IFITM3 is protective in SARS-CoV-2 infections of mice.
Identifying mutations in sd1, Pi54 and Pi-ta, and positively selected genes of TN1, the first semidwarf rice in Green Revolution
Authors: Panibe Jerome P. , Wang Long , Lee Yi-Chen , Wang Chang-Sheng , Li Wen-Hsiung 
Published: December 2021

AbstractBackgroundTaichung Native 1 (TN1) is the first semidwarf rice cultivar that initiated the Green Revolution. As TN1 is a direct descendant of the Dee-geo-woo-gen cultivar, the source of the sd1 semidwarf gene, the sd1 gene can be defined through TN1. Also, TN1 is susceptible to the blast disease and is described as being drought-tolerant. However, genes related to these characteristics of TN1 are unknown. Our aim was to identify and characterize TN1 genes related to these traits.ResultsAligning the sd1 of TN1 to Nipponbare sd1, we found a 382-bp deletion including a frameshift mutation. Sanger sequencing validated this deleted region in sd1, and we proposed a model of the sd1 gene that corrects errors in the literature. We also predicted the blast disease resistant (R) genes of TN1. Orthologues of the R genes in Tetep, a well-known resistant cultivar that is commonly used as a donor for breeding new blast resistant cultivars, were then sought in TN1, and if they were present, we looked for mutations. The absence of Pi54, a well-known R gene, in TN1 partially explains why TN1 is more susceptible to blast than Tetep. We also scanned the TN1 genome using the PosiGene software and identified 11 genes deemed to have undergone positive selection. Some of them are associated with drought-resistance and stress response.ConclusionsWe have redefined the deletion of the sd1 gene in TN1, a direct descendant of the Dee-geo-woo-gen cultivar, and have corrected some literature errors. Moreover, we have identified blast resistant genes and positively selected genes, including genes that characterize TN1’s blast susceptibility and abiotic stress response. These new findings increase the potential of using TN1 to breed new rice cultivars.
Two doses of mRNA vaccine elicit cross-neutralizing memory B-cells against SARS-CoV-2 Omicron variant
Authors: Kotaki Ryutaro , Adachi Yu , Moriyama Saya , Onodera Taishi , Fukushi Shuetsu , Nagakura Takaki , Tonouchi Keisuke , Terahara Kazutaka , Sun Lin , Takano Tomohiro , Nishiyama Ayae , Shinkai Masaharu , Oba Kunihiro , Nakamura-Uchiyama Fukumi , Shimizu Hidefumi , Suzuki Tadaki , Matsumura Takayuki , Isogawa Masanori , Takahashi Yoshimasa 
Published: December 2021

AbstractSARS-CoV-2 Beta and Omicron variants have multiple mutations in the receptor-binding domain (RBD) allowing antibody evasion. Despite the resistance to circulating antibodies in those who received two doses of mRNA vaccine, the third dose prominently recalls cross-neutralizing antibodies with expanded breadth to these variants. Herein, we longitudinally profiled the cellular composition of persistent memory B-cell subsets and their antibody reactivity against these variants following the second vaccine dose. The vaccination elicited a memory B-cell subset with resting phenotype that dominated the other subsets at 4.9 months. Notably, most of the resting memory subset retained the ability to bind the Beta variant, and the memory-derived antibodies cross-neutralized the Beta and Omicron variants at frequencies of 59% and 29%, respectively. The preservation of cross-neutralizing antibody repertoires in the durable memory B-cell subset likely contributes to the prominent recall of cross-neutralizing antibodies following the third dose of the vaccine.One Sentence SummaryFully vaccinated individuals preserve cross-neutralizing memory B-cells against the SARS-CoV-2 Omicron variant.
Structure, dynamics, and inhibition of Staphylococcus aureus m1A22-tRNA methyltransferase
Authors: Sweeney Pamela , Crowe Ashleigh , Kumar Abhishek , Raju Dinesh , Krishna Naveen B. , Sutherland Emmajay , Leo Caitlin J. , Fisher Gemma , Lalitha Roopa , Muthuraj Likith , Sigamani Gladstone , Oehler Verena , Synowsky Silvia , Shirran Sally L. , Gloster Tracey M. , Czekster Clarissa M. , Kumar Pravin , da Silva Rafael G. 
Published: December 2021

AbstractThe enzyme m1A22-tRNA methyltransferase (TrmK) catalyses the transfer of a methyl group from SAM to the N1 of adenine 22 in tRNAs. TrmK is essential for Staphylococcus aureus survival during infection, but has no homologue in mammals, making it a promising target for antibiotic development. Here we describe the structural and functional characterisation of S. aureus TrmK. Crystal structures are reported for S. aureus TrmK apoenzyme and in complexes with SAM and SAH. Isothermal titration calorimetry showed that SAM binds to the enzyme with favourable but modest enthalpic and entropic contributions, whereas SAH binding leads to an entropic penalty compensated by a large favourable enthalpic contribution. Molecular dynamics simulations point to specific motions of the C-terminal domain being altered by SAM binding, which might have implications for tRNA recruitment. Activity assays for S. aureus TrmK-catalysed methylation of WT and position 22 mutants of tRNALeu demonstrate that the enzyme requires an adenine at position 22 of the tRNA. Intriguingly, a small RNA hairpin of 18 nucleotides is methylated by TrmK depending on the position of the adenine. In-silico screening of compounds suggested plumbagin as a potential inhibitor of TrmK, which was confirmed by activity measurements. Furthermore, LC-MS indicated the protein was covalently modified by one equivalent of the inhibitor, and proteolytic digestion coupled with LC-MS identified Cys92, in the vicinity of the SAM-binding site, as the sole residue modified. These results these results identify a cryptic binding pocket of S. aureus TrmK and lay the foundation for future structure-based drug discovery.
Graphene Oxide reinforced Agarose-Hydroxyapatite Bioprinted 3-D Scaffolds for Bone Regeneration
Authors: Yadav Umakant , Kumar Shiva 
Published: December 2021

AbstractThree-dimensional (3D) bioprinting is an emerging technology for fabricating cells, biomaterials and extracellular matrix (ECM) into customized shapes and patterns. Here, we report additive manufacturing to create a customized 3D bioactive constructs for regenerative medicine. We have attempted to emphasize the use of agarose and graphene oxide as a promising material for the conceptualization of bioink unpaid to its unique physicochemical properties. The 3D printed structure is able to regenerating bone tissues and regulates the cellular differentiation without any significant morphological changes. The presence of graphene oxide enhances the osteoinductive behavior of the developed scaffolds, which is further supplemented by encapsulating human mesenchymal stem cells (hMSCs) on the 3D printed scaffolds.A significant enhanced expression of early osteogenic markers like morphogenetic protein (BMP), Runx-2, collagen-1, osteopontin, osteocalcin as well as mineralized ECM are observed on agarose-hydroxyapatite and graphene oxide 3D printed scaffolds compared to agarose-hydroxyapatite 3D printed scaffolds. Thus, the outcomes of the developed 3D bioprinted scaffolds provide a promising strategy for development of personalized bone grafts for tissue regeneration.
The mediating role of competitive orientation in determining self-construal in unethical decision-making
Authors: Dissanayake D.M. Sachinthanee 
Published: December 2021

PurposeThe ethics literature has focused on the influence of self-construal dimensions on unethical decision-making. However, the literature is unclear about why these self-construal dimensions (Independent-self, Relational-self, Collective-self) impact differently on unethical decision-making. Based on the theory of cooperation and competition, this study empirically examines the mediating role of competitive orientation and addresses the theoretically unexplained question of why self-construal dimensions influence differently on unethical decision-making.Design/methodology/approachBased on the deductive approach, a quantitative research study was conducted on the Sri Lankan banking industry because there have been many instances of unethical behavior reported in this sector lately. Data were collected from 305 bank branch managers using a structured survey questionnaire.FindingsThe findings revealed that competitive orientation mediates the self-construal dimensions and explained that competitive orientation is one reason why independent-self, relational-self and collective-self influence differently on unethical decision-making.Originality/valueThis paper addresses the unanswered question of why self-construal dimensions relate to unethical decision-making differently.
Work values: a latent class analysis of Korean employees
Authors: Lee Jinju , Lee Yunsoo , Kim Soo Jung , Song Ji Hoon 
Published: December 2021

PurposeThe purpose of this study is to identify latent classes of work values that influence the career choices of Korean workers through a person-centred approach. Because work values may be diverse in individuals, investigating the various combinations of those values will help understand individual's decisions to take, maintain and leave a job.Design/methodology/approachThe authors used a total of 15,103 datasets collected at the national level in South Korea. Data were analyzed by using latent class analysis (LCA); a three-step approach was employed to address classification.FindingsAs the results of this study, Class 3, “seeking balanced work values rather than income,” presented the highest level of job satisfaction, whereas Class 1, “seeking job security and income,” exhibited the lowest.Originality/valueEmploying an assessment tool to identify the distinct combinations of work values that individuals possess and then providing them with appropriate training and development programs and customized Human Resource (HR) policies aligned with the classes will be essential tasks for HRD (Human Resource Development)/HRM (Human Resource Management) practitioners.
Consumer responses to the failure of self-service banking technology: moderating role of failure stability
Authors: Bahri Ammari Nedra , Hsouna Abir , Benabdallah Mounia , Yousaf Anish , Mishra Abhishek 
Published: December 2021

PurposeThe purpose of this study is to investigate the impact of dissatisfaction and anger, driven by the failure of the self-service technology of banks, on customers' post-purchase behavioural reactions, such as complaints, negative word-of-mouth (NWOM) and supplier change. The stability of the failure is proposed to moderate these relationships.Design/methodology/approachThe proposed research model was tested through data collected from an online survey of a Tunisian sample of 300 respondents, using the scenario method.FindingsThe study validates the positive impact of dissatisfaction on anger and negative word-of-mouth, as well as that of anger on complaint behaviour and negative word-of-mouth. The relation between dissatisfaction and negative word-of-mouth is mediated by anger. When the failure is stable, dissatisfied users of the self-service technology seek to enhance their negative word-of-mouth and supplier change. The results also show that the stability of the failure enhances the effect of anger on complaint behaviour.Practical implicationsBanks should invest efforts to accelerate the recovery of services to reduce consumer dissatisfaction and anger and prevent adverse behavioural outcomes. Further, they need to ensure that failures are not repeated, as failure stability activates some otherwise non-significant behavioural outcomes, like supplier change.Originality/valuePrevious works have focused on the impact of dissatisfaction and negative emotions for interpersonal services, but very few works have come to associate dissatisfaction, anger, complaint, negative word-of-mouth and supplier change in an integrative framework for an self-service technology failure.
Female entrepreneurship in emerging and developing countries: a systematic literature review
Authors: Corrêa Victor Silva , Brito Fernanda Regina da Silva , Lima Rosileine Mendonça de , Queiroz Maciel M. 
Published: December 2021

PurposeDespite the increase in female entrepreneurship literature, very few studies exist that systematize the extant literature, especially in emerging and developing countries. This article fills part of this gap; it maps, categorizes and groups the objectives, theoretical approaches and research methods on female entrepreneurship conducted in one or more of the 155 emerging and developing countries.Design/methodology/approachA systematic literature review (SLR) was conducted, using Scopus and Web of Science, over a 10-year timeframe (2010–2020). Out of 465 papers, 77 were selected for content analysis.FindingsMost articles focus on understanding women entrepreneurs' challenges, the factors affecting their entrepreneurial performance and encouraging entrepreneurship. Qualitative research was found to be the predominant approach, while mixed studies appeared less frequently.Practical implicationsThis paper sheds light on female entrepreneurship characteristics, including business competence, performance and entrepreneurial orientation. Further, it can help female entrepreneurs to recognize the most relevant aspects regarding performance, the essential driving factors and entrepreneurial motivations, among others.Originality/valueFirst, this paper groups the objectives and the theoretical and methodological approaches that guide female entrepreneurship research. Second, it identifies distinct gaps, grouped and explored using unpublished thematic categories. Finally, the authors propose an extensive future research agenda regarding female entrepreneurship in emerging and developing countries.
Market knowledge acquisition and transfer in international SMEs: peculiarities, specifications and linkages to market success
Authors: Siachou Evangelia , Papasolomou Ioanna , Trichina Eleni , Thrassou Alkis 
Published: December 2021

PurposeThis paper aims to systematically review and evaluate extant literature on knowledge acquisition and transfer in international small-to-medium enterprises (SMEs) and to identify the requisite types of knowledge acquired and transferred in this context. The research further determines the mechanisms enabling SMEs to process this knowledge, unpacks the benefits of both knowledge acquisition and transfer for international SMEs and links them to the market success.Design/methodology/approachThe research has conducted a comprehensive systematic review of existing literature on market knowledge acquisition and its transfer, in the context of international SMEs, utilizing peer-reviewed articles published in top tier journals without any custom range of time.FindingsThe search strategy resulted in 37 reviewed academic articles, whose analysis identifies and elucidates on the best practices of knowledge acquisition and transfer in the context of international SMEs; the type of the knowledge acquired and transferred; the form and mechanism of the intersection of these processes; and the benefits gained. The findings, crucially, also identify and illuminate extant research gaps and insufficiencies and develop a comprehensive research agenda for the way forward.Originality/valueThe present study offers a uniquely comprehensive perspective on a topic of diachronic and, currently, rising significance to scholars and practitioners alike. Transcending its descriptive value, though, it further identifies extant knowledge deficiencies, it distils and consolidates critical knowledge and prescribes avenues for research towards the growth and development of international SMEs and born globals.
Market overreaction, firm-specific information and macroeconomic variables in US and Chinese markets during COVID-19
Authors: Loang Ooi Kok , Ahmad Zamri 
Published: December 2021

PurposeThis study examines the impact of firm-specific information and macroeconomic variables on market overreaction of US and Chinese winner and loser portfolio before and during COVID-19.Design/methodology/approachThe firm-specific information includes firm size, volume, volatility, return of asset (ROA), return of equity (ROE), earning per share (EPS) and quick ratio while the macroeconomic variables are export rate, import rate, real GDP, nominal GDP, FDI, IPI and unemployment rate. Besides, one-third of the top performance stocks are categorized as winner portfolio while one-third of lowest performance stocks are categorized as loser portfolio. This study uses AECR to indicate stock return and measure market overreaction. GAECR is used to determine contrarian profit. The data range of pre-COVID-19 is from 1-Jan-2015 to 31-Dec-2019 while the period of COVID-19 is from 1-Jan-2020 to 31-Dec-2020.FindingsIn pre-COVID-19, firm-specific information (volatility, ROA, ROE and EPS) and macroeconomic variables are found to be correlated to stock return in US and Chinese portfolios except Chinese winner portfolio. Nonetheless, the impact of firm-specific information has vanished and macroeconomic variables are significant to stock return in COVID-19. It shows that investors rely on the economic indicators to trade in turbulent period due to emergence of COVID-19 as a disruption in market. Furthermore, US and Chinese portfolios are overreacted during COVID-19. Chinese loser portfolio has higher tendency of overreaction than US loser portfolio while US winner portfolio has higher tendency of overreaction than Chinese winner portfolio.Originality/valueThe results of this study assists academician, practitioners and investors on understanding and create awareness to the existence of market overreaction and the determinants that can cause the phenomenon.
Towards quality primary health care: the dilemma of Community-Based Health Planning and Services (CHPS) in health service provision in Ghana
Authors: Addi Barnabas , Doe Benjamin , Oduro-Ofori Eric 
Published: December 2021

PurposeOver the past two decades, Community-Based Health Planning and Services (CHPS) has been a pragmatic strategy towards universal Primary Health Care (PHC) in Ghana. However, the ability and capacity of these facilities to deliver quality primary health care remain an illusion as they are still crumbling in myriad challenges. These challenges are translated to the poor-quality services provision and low community utilization of CHPS facilities. The study presents a comparative analysis of three communities in the Kassena-Nankana East Municipality, Ghana.Design/methodology/approachUsing a mixed-method research design, the study gathered and analysed data from 110 households, three community health officers (CHOs) and three community leaders using semi-structured questionnaires and interview guides.FindingsThe findings indicated that the facilities do not have the requisite inputs such as drugs and supplies, logistics, appropriate health personnel, good infrastructure, funding support necessary to deliver quality and appropriate healthcare services that meet the health needs of the communities. For the CHPS to realize their full potentials as PHC facilities, it is required that the needed inputs such as logistics, drugs and appropriate staff are in place to facilitate the activities of CHOs.Research limitations/implicationsDue to the limited number of participants and selection of the study communities, the results may generalization. Also, the researchers acknowledged the inability to interview the district level health officials and the Kassena-Nankana Municipal Assembly during the field visits. This could have provided in-depth knowledge on the findings of this research as well as the validation of the results from the communities' perspective. Several attempts were made to contact and interview district-level authorities which proven futile due to the unavailability of targeted respondents. This resulted in limiting the studies at the community level. However, this limitation does not disprove the findings of this study.Practical implicationsThe article implications for planning primary health care strategies include a keen assessment of community health needs and institutional management of primary health care facilities, equip PHC facilities with adequate resources such as drugs and appropriate staffing to provide the health needs of the communities.Originality/valueThe paper fulfils the gap in the literature by providing empirical data on how the challenges of primary health care facilities affected the provision of high quality service and how this can affect community’s use of the facilities.
Power law bond price and yield approximation
Authors: Barber Joel R. 
Published: December 2021

PurposeThis paper determines a simple transformation that nearly linearizes the bond price formula. The transformed price can be used to derive a highly accurate approximation of the change in a bond price resulting from a change in interest rates.Design/methodology/approachA logarithmic transformation exactly linearizes the price function for a zero coupon bond and a reciprocal transformation exactly linearizes the price function for a perpetuity. A power law transformation combines aspects of both types of transformations and provides a superior approximation of the bond price sensitivity for both short-term and long-term bonds.FindingsIt is demonstrated that the new formula, based on power-law transformation, is a much better approximation than either the traditional duration-convexity approximation and the more recently developed approximations based on logarithmic transformation of the price function.Originality/valueThe new formula will be used by risk managers to perform stress-testing on bond portfolios. The new formula can easily be inverted, making it possible to relate the distribution of prices (which are observable in the market) to the distribution of yields (which are numerical solutions that are not directly observable).