bluuebunny/crossref_metadata_2025_split huggingface dataset:
Idiosyncratic Functions of Active Touch Strategies in Shape Perception.
Authors: Mizrachi Neomi , Nelinger Guy , Ahissar Ehud , Arieli Amos 
Published: May 2021

Abstract
Hand movements are essential for tactile perception of objects. However, the specific functions served by active touch strategies, and their dependence on physiological parameters, is unclear and understudied. Focusing on planar shape perception, we tracked at high resolution the hands of eleven participants during shape recognition task. Two dominant hand movements strategies were identified: Contour-following movements, either tangential to the contour or oscillating perpendicular to it, and exploration by scanning movements, crossing between distant parts of the shapes’ contour. Both strategies exhibited non-uniform coverage of the shapes’ contours. Idiosyncratic movement patterns were specific to the sensed object and could be explained in part by spatial and temporal tactile thresholds of the participant. Using simulations, we show how some strategy choices may affect receptors activation. These results suggest that motion strategies of active touch adapt to both the sensed object and to the perceiver’s physiological parameters.
Google-Reflected Information on Drugs Used for Anesthesia: Time Course of Growth in Number of Web Pages
Authors: Vlassakov Kamen V. , Kissin Igor 
Published: May 2021

Abstract
Purpose: The aim of this study was to determine the time course of growth in general Google-reflected information on drugs used for anesthesia. As a contrast to the changes in general Google-reflected information we used the changes in academic PubMed-reflected information.Methods: General Google-reflected information on anesthetics was assessed by counting the number of Google Web pages. Academic information was assessed by counting the number of articles in medico-biological journals covered by the PubMed database (The National Library of Medicine). The ratio of Google Web pages to PubMed articles (G/P Ratio) was used to indicate prevalence of Google-related information. Twenty-five agents used for anesthesia were selected from three pharmacological groups – general anesthetics, local anesthetics, and opioids -- based on the frequency of their association with anesthesia in academic medical journals. The time course of growth in general Google-reflected information was determined for seven 5-year periods, from 1983 to 2017. Results: With the growing role of the Web, the number of Google Web pages on drugs used for anesthesia increased rapidly. As a result, the relationship between general Google-reflected and academic PubMed-reflected information on anesthetics profoundly changed. Before the 1993-1997 period, the number of Google Web pages on anesthetics was only a fraction of the number of PubMed articles. By the 2013-2017period, the relationship was completely reversed: for any anesthetic, the number of Google Web pages was at least three times greater than the number of PubMed articles. However, the relationship of general Web-related information and academic information with different anesthetics was very variable. In 2013-2017, the G/P Ratio, indicating the magnitude of general information dominance, for the 25 agents varied from 3.0 (remifentanil) to 23.2 (oxycodone). The dominance of Google information was especially profound with drugs that have a wider spectrum of possible use beyond the field of anesthesia, such as oxycodone or diazepam.Conclusion: General Google-reflected information is rapidly growing and, as a result, its dominance over academic PubMed-reflected information is constantly increasing.
Metagenomics Study of Fungi and Fungi-Like Organisms Associated With the Seagrass Halophila Stipulacea (Forssk.) Asch. From Al-Leith Mangroves, Saudi Arabia
Authors: Abdel-Wahab Mohamed , Bahkali Ali H. , El-Gorban Abdallah M , Jones Gareth 
Published: May 2021

Abstract
This is the first metagenomics study of the fungal community associated with leaves of the seagrass Halophila stipulacea. Five leave samples were collected from the Al-Leith mangroves along the Red Sea coast of Saudi Arabia. Total DNA was extracted from 250 mg of each sample and the first 300 bp (contains D1-D2 variable regions) of the LSU rDNA amplicon was sequenced with the Illumina MiSeq (bTEFAP). A total of 928,626 reads were obtained from the five samples. The sequence reads belonged to the kingdoms: Metazoa (48.1% of the total reads), Viridiplantae (41.1%), Eukaryota (8.8%), Fungi (1.96%), Bacteria (0.09%), and Archaea (0.0001%). Fungi represented between 1.1% and 5.8% of the total reads in the five samples. A total of 18,279 reads (representing 1.96% of the total reads) were recorded from the 5 samples representing 296 molecular species (OTUs) that belong to 13 fungal phyla. At the phylum level, Basidiomycota dominated the community (37.2–51.6%) in three samples, while Neocallimastigomycota (37.5%) and Mucoromycota (42.1%) dominated the community in the fourth and the fifth sample respectively. High diversity of OTUs (28 molecular species) were recorded from the monokaryotic subkingdom with five unknown basal lineages that are not aligning with any known taxa. Total number of sequence reads of fungi-like organisms (Stramenopiles) from the five samples ranged between 0.16% of total reads in the fifth sample (AL-Hs05) to 2.9% in the first one (AL-Hs01). Majority of the fungi-like organisms reads (93.6%) belong to the phylum Oomycota, followed by Opisthokonts (Fungi/Metazoa group) representing 6.4% of fungi-like reads. Monokaryon phyla (i.e. Chytridiomycota, Mucoromycota and Neocallimastigomycota) and fungi-like organisms occupied a major portion of the sequences reads followed by Basidiomycota and Ascomycota. Our results support findings that the majority of fungi and fungi-like organisms’ communities are so far unknown with seven deep branching lineages remain to be cultivated.
Mosquito-vertebrate Interaction Networks in the Magdalena Medio Region of Colombia
Authors: Hoyos Juliana , Carrasquilla María Cristina , Leon Cielo , Montgomery Joel M. , Salyer Stephanie J. , Komar Nicholas , González Camila 
Published: May 2021

Abstract
Studies on the feeding behavior of hematophagous insects, particularly those of medical importance, are relevant for tracking possible pathogen transmission routes and identifying biases in the choice of vertebrates. We evaluated host selection of blood-feeding mosquitoes in a disturbed forest in the Magdalena Medio valley in Colombia from March 2017 to April 2018, after the introduction of Zika virus to the Americas from the 2015-2016 outbreak. We estimated vertebrate diversity and collected blood-engorged female mosquitoes. Mosquito genomic DNA/RNA was extracted for vertebrate host identification and pathogen detection. We performed conventional PCR and sequencing, using universal primers targeting vertebrate regions of the eukaryotic mitochondrial genome to determine bloodmeal host. Additionally, we tested for the presence of flaviviruses in all mosquito samples with RT-PCR. Based on the identity and quantity of detected bloodmeals, we performed mosquito-vertebrate interaction network analysis and estimated topology metrics. In total, we collected 292 engorged female mosquitoes representing 20 different species. Bloodmeal analyses identified 26 vertebrate species, the majority of which were mammals (N=16; 61.5%). No flavivirus infection was confirmed. Although feeding patterns varied, network analyses showed a high degree of specialization by mosquitoes and revealed ecological and phylogenetic relationships among the host community. We conclude that host selection or preference by mosquitoes is species specific.
Impact of the Life-Sustaining Treatment Decision Act on Brain Death Organ Tissue Donation in Korea: A Qualitative Study of Emergency Physicians’ Experiences and Perspectives
Authors: Park Song Yi , Kim Hyun , Park Kyung Hye , Park Kwi Hwa , Park Seung Min , Lee Dong Eun , Jung Yong Hun , Jeong Won Joon 
Published: May 2021

Abstract
Background: In Korea, a new law was enacted in January 2016 allowing life-sustaining treatment (LST) of patients in end-of-life to be stopped, popularly known as the LST Decision Act. It came into effect in February 2018. Since then, brain death organ tissue donation affiliated with emergency physicians has decreased. This study aimed to explore emergency physicians’ experiences of and perspectives toward the impact of the LST Decision Act on brain death in Korea. Methods: A qualitative study was conducted using thematic analysis methods. Ten emergency physicians with experience in brain death, organ tissue donation, and LST were interviewed. Data analysis was performed following the six steps proposed by Braun and Clarke. Results: Data analysis revealed 14 subthemes and 5 themes: 1) The LST Decision Act is a legitimate way to stop LST, but it is not enough; 2) Many family members stop LST first, often more quickly and actively than physicians expect them to; 3) Stopping LST makes the patient more comfortable; thus, caregivers do not wish to opt for organ tissue donation, which is seen as distressing; 4) Not all patients experience brain death, and LST is stopped before brain death; and 5) Since the LST Decision Act, brain death organ tissue donation has decreased. Conclusion: Emergency physicians generally welcomed the LST Decision Act. However, family members seemed to perceive stopping LST as the means to making the patients comfortable, resulting in the loss of potential organ tissue donors. There is a need for a supplementary policy that connects stopping LST to brain death organ tissue donation.
Intraepineurial Fat Quantification and Cross-sectional Area Analysis of the Sciatic Nerve Using MRI in Charcot-Marie-Tooth Disease Type 1A Patients
Authors: Kim Hyun Su , Lee Ji Hyun , Yoon Young Cheol , Cha Min Jae , Nam Soo Hyun , Kwon Hye Mi , Kim Seonwoo , Won Hojeong , Choi Byung-Ok 
Published: May 2021

Abstract
The objectives of this study were to assess the fat fraction (FF) and cross-sectional area (CSA) of the sciatic nerve in Charcot-Marie-Tooth disease type 1A (CMT1A) patients using Dixon-based proton density fat quantification MRI and to elucidate its potential association with clinical parameters. Thigh MRIs of 18 CMT1A patients and 18 age- and sex-matched volunteers enrolled for a previous study were reviewed. Analyses for FF and CSA of the sciatic nerve were performed at three levels (proximal to distal). CSA and FF were compared between the two groups and among the different levels within each group. The relationship between the MRI parameters and clinical data were assessed in the CMT1A patients. The CMT1A patients showed significantly higher FF at level 3 (p = 0.0217) and significantly larger CSA at all three levels compared with the control participants (p < 0.0001). Comparisons among levels showed significantly higher FF for levels 2 and 3 than for level 1 and significantly larger CSA for level 2 compared with level 1 in CMT1A patients. CSA at level 3 correlated positively with the CMT Neuropathy Score version 2 (CMTNSv2). In conclusion, the sciatic nerve FF of CMT1A patients was significantly higher on level 3 compared with both the controls and the measurements taken on more proximal levels, suggesting the possibility of increased intraepineurial fat within the sciatic nerves of CMT1A patients, with a possible distal tendency. Sciatic nerve CSA at level 3 correlated significantly and positively with CMTNSv2, suggesting its potential value as an imaging marker for clinical severity.
Discriminating uninfected surgical bed cysts from bacterial brain abscesses after Carmustine wafer implantation in newly diagnosed IDH-wildtype glioblastomas
Authors: Roux Alexandre , Ammar Hichem , Moiraghi Alessandro , Peeters Sophie , Baroud Marwan , Zah-Bi Gilles , Benzakoun Joseph , Parraga Eduardo , Oppenheim Catherine , Benevello Chiara , Chrétien Fabrice , Varlet Pascale , Dhermain Frédéric , Dezamis Edouard , Zanello Marc , Pallud Johan 
Published: May 2021

Abstract
PurposeCarmustine wafers can be implanted in the surgical bed of high-grade gliomas, which can induce surgical bed cyst formation, leading to clinically relevant mass effect.MethodsAn observational retrospective monocentric study was conducted including 122 consecutive adult patients with a newly diagnosed supratentorial glioblastoma who underwent a surgical resection with Carmustine wafer implantation as first line treatment (2005–2018).FindingsTwenty-two patients (18.0%) developed a postoperative contrast-enhancing cyst within the surgical bed: 16 uninfected cysts and six bacterial abscesses. All patients with an uninfected surgical bed cyst were managed conservatively, all resolved on imaging follow-up, and no patient stopped the radiochemotherapy. Independent risk factors of formation of a postoperative uninfected surgical bed cyst were age ≥ 60 years (p = 0.019), number of Carmustine wafers implanted ≥ 8 (p = 0.040), and partial resection (p = 0.025). Compared to uninfected surgical bed cysts, the occurrence of a postoperative bacterial abscess requiring surgical management was associated more frequently with a shorter time to diagnosis from surgery (p = 0.009), new neurological deficit (p < 0.001), fever (p < 0.001), residual air in the cyst (p = 0.018), a cyst diameter greater than that of the initial tumor (p = 0.027), and increased mass effect and brain edema compared to early postoperative MRI (p = 0.024). Contrast enhancement (p = 0.473) and diffusion signal abnormalities (p = 0.471) did not differ between postoperative bacterial abscesses and uninfected surgical bed cysts.ConclusionsClinical and imaging findings help discriminate between uninfected surgical bed cysts and bacterial abscesses following Carmustine wafer implantation. Surgical bed cysts can be managed conservatively. Individual risk factors will help tailor their steroid therapy and imaging follow-up.
Telemedicine application in patient with Diabetes, Hypertension and Rheumatoid arthritis: A systematic review and meta-analysis
Authors: Ma Yue , Zhao Yan , Zhao Chongbo , Lu Jiahong , Jiang Hong , Cao Yanpei , Xu Yafang 
Published: May 2021

Abstract
Background: Under the global epidemic condition，the telemedicine was widely used in the world, especially in the period of long-term care and treatment. The aim of study is to verify the effectiveness of telemedicine in the management of chronic disease by using scientific methods such as hypertension, diabetes, rheumatoid arthritis by using Meta-analysis and systematic review methods. The purpose of this study was to systematic review of the effect of telemedicine in chronic disease, so as to provide inspiration for chronic disease management in the future.Methods: Article searching were performed using Web of Science, PubMed, MEDLINE, EMBASE and other library or database to retrieve articles which published from database and library establishment to October 31st, 2020. Literature quality assessment, systematic review and Meta-analysis were then performed.Results: 12 articles were included in the literature quality assessment (7 diabetes,3 hypertensions,2 rheumatoid arthritis), the article included in this study is of high quality. There are 7 articles included in the Meta-analysis, the result shows there have effect in glycosylated hemoglobin after 12 months of intervention (95%CI=-1.53, -0.16; Z=2.42; P=0.02), compare with 6 months (95%CI=-1.32, -0.01; Z=1.99; P=0.05). It also showed that there was no significant difference in fasting blood glucose after 6 months of intervention (95%CI=-1.19,0.21; Z=1.37; P=0.17). Both systolic blood pressure (95%CI=-12.79, -3.69; Z=3.55; P= 0.0004) and diastolic blood pressure (95%CI=-9.90, -0.43; Z=2.14; P= 0.03) showed statistically significant. Moreover, we also found positive influence of telemedicine about good behaviors and rehabilitation for rheumatoid arthritis patients.Conclusion: The results showed that telemedicine had a positive effect on the management of diabetes, hypertension and rheumatoid arthritis, especially on the management of glycosylated hemoglobin and blood pressure. Telemedicine technologies have great promotion to access the medical services and improve the quality of care, especially for people with chronic diseases.
Prognostic Impact of Tumor Budding on Moroccan Colon Cancer Patients
Authors: agy Fatima El , bardai Sanae El , Bouguenouch Laila , Lahmidani Nada , abkari Mohammed El , Benjelloun El bachir , ousadden Abdelmalek , Mazaz Khalid , Taughrai Imane , Ibrahimi Sidi adil , Benbrahim Zineb , Chbani Laila 
Published: May 2021

Abstract
Background: Tumor budding is now emerging as one of the robust and promising histological factors that play an important role in colon cancer. In this study, we aimed to investigate the association between tumor budding and tumor clinicopathological factors, tumor molecular signature, and patient survival for the first time in the Moroccan population. Methods: We collected data of 100 patients with operated colon adenocarcinoma. Tumor budding was assessed on HES slides, according to the International Tumor Budding Consensus Conference 2016 recommendations. The expression of MMR proteins was performed by Immunohistochemistry. KRAS and NRAS mutations testing was performed by Sanger sequencing and pyrosequençing. Results: High tumor budding grade (BUD 3) was found to be significantly associated with adverse clinicopathological features including older age (P=0.03), presence of perineural invasion (P=0.02), presence of vascular invasion (P=0.05), distant metastases (P˂0.001), advanced TNM stage (P=0.001), the occurrence of relapse (P=0.04), and the high number of deceased cases (P=0.02). Interestingly, we found that tumors with high budding were more likely to be microsatellite stable (MSS) (P=0.005) and harbor more KRAS mutations (P=0.02). In all stages, High tumor budding was correlated with poorer overall survival (P=0.04) and decreased relapse-free survival with a difference close to significance ((P=0.09). we concluded that high tumor budding was strongly associated with unfavorable clinicopathological features and special molecular biomarkers and effectively affects the overall survival of CC patients. Conclusions: Based on these findings and the ITBCC group recommendations, tumor budding should be taken into account along with other clinicopathologic factors in the risk assessment of colorectal cancer.
Multiplex Detection of Rotavirus A, B, C, Norovirus GI and GII, Adenovirus, Astrovirus and Sapovirus in a Single PCR System in Stool Samples From Children With Acute Diarrhea
Authors: Li Wei , Li Wei-wei , Li Lin , He Lin , Xiang Wen-qing , Chen Jie , Mao Jian-hua 
Published: May 2021

Abstract
We developed a RT-PCR combined with melting curve analysis (RRCMC) method for simultaneous detection of rotavirus A, B, C, norovirus GI and GII, adenovirus, astrovirus and sapovirus. Stool samples were collected from 160 children with acute diarrhea and tested by RRCMC assay. A total of 71 patients were tested positive with norovirus, adenovirus or rotavirus. The RRCMC assay has high specificity. There is no internal cross-reaction through the 8 diarrhea viruses and no cross-reaction of other commonly intestinal pathogens and human genome. The detection limit was ranging from 1×102 to 1×105 copies/ml for each diarrhea virus. In conclusion, the RRCMC method is a suitable rapid clinical test for infectious viruses, with the advantages of high-throughput, low cost, high sensitivity and specificity.
Trajectory of Bearing Movement During Oxford Mobile-bearing Unicompartmental Knee Arthroplasty Using Kinematic Alignment Technique: a Retrospective Analysis
Authors: Sun Xiaowei , Lu Feifan , Wang Weiguo , Cheng Liming , Guo Wanshou , Zhang Qidong 
Published: May 2021

Abstract
Background: This study was performed to clarify whether the kinematic alignment technique can improve the trajectory of the bearing movement during Oxford mobile-bearing unicompartmental knee arthroplasty (Oxford MB UKA) compared with the traditional technique using a Microplasty instrument.Methods: We retrospectively analyzed patients who underwent UKA from January to June 2019. The kinematic alignment technique group (study group) comprised 23 patients (30 knees), and the Microplasty group (control group) comprised 25 patients (30 knees). The clinical outcomes, radiographic assessments, and intraoperative bearing movement trajectory and distance were compared between the two groups.Results: There were no differences in the baseline characteristics or clinical outcomes between the two groups (P > 0.05). There were also no differences in the postoperative femoral and tibial prosthesis varus and valgus angles, femoral prosthesis flexion and extension angles, tibial slope, or prosthesis convergence angle (P > 0.05). The contiguity between the femoral and tibial prosthesis in the study group was 4.8 ± 1.6 mm, while that in the control group was 6.3 ± 1.8 mm (P < 0.05). The difference in the bearing movement trajectory was significant, with an ideal trajectory in 23/30 cases in the study group and 14/30 cases in the control group (P < 0.05). The bearing movement distance showed no significant difference between the groups (P > 0.05).Conclusion: The prosthesis installation accuracy of the kinematic alignment technique in Oxford MB UKA was similar to that of conventional Microplasty instrumentation. However, the contiguity between the femoral and tibial prosthesis was smaller, and the bearing movement trajectory was more optimal.
Genetic Characterization of Plasmid-mediated qepA Gene Among ESBL-producing Escherichia Coli Isolates in Mexico
Authors: Barrios-Camacho Humberto , Duran-Bedolla Josefina , Silva-Sanchez Jesus , Lozano-Aguirre Luis , Reyna-Flores Fernando , Sanchez-Perez Alejandro , Graza-Ramos Ulises 
Published: May 2021

Abstract
A molecular characterization of a plasmid-born qepA gene in (ESBL)-producing E. coli clinical isolates were performed. An 2.63% (11/418) were qepA positive isolates, of which a 90.0% carried CTX-M-15 (9/11) and SHV-12 (1/11). All isolates showed chromosomal mutations in the gyrA and parC genes. The clonal groups A, B and C were identified and belonged to, respectively, phylogroups A, B1 and D, as well as the sequence types 205, 405 and 617. Several plasmid profiles were determined with incompatibility groups FIA, FIB and FII. The genetic environment of the qepA in plasmid pEC8020 was different from those reported previously. The plasmid sequence included genes conferring resistance to β-lactams (blaCTX-M-15), macrolides (mphA), fluoroquinolones (qepA1), trimethoprim (dfrB4) and sulphonamides (sul1). Likewise, the IncF-pEC8020 plasmid carried several insertion sequences including ISCR3, IS6100 and multiple copies of IS26. This work contributes to the epidemiology and genetics of plasmid-born qepA genes of ESBL-producing E. coli.
Enhanced electrochemical water splitting activity in annealed TiO2 nanoparticles of photoanode
Authors: Nguyen Tien Thanh , Dao Khac An , Luong Xuan Dien , Ung Thi Dieu Thuy 
Published: May 2021

Abstract
In this paper, we present the electrochemical water splitting characteristics of TiO2/FTO electrodes via spin-coating method. By using thermal annealing approach, the TiO2 nanoparticle (P25) was modified with a more active photocatalyst. The annealed TiO2 nanoparticle-based photoanode in vacuum shows photocurrent density of 0.27 mA/cm2 and photoconversion efficiency of (η = 0.22%) at potential of 0.4 V (vs. RHE), which are higher than those of annealed TiO2 nanoparticle-based electrode in air. The improved photoelectrochemical property is attributed to high oxygen vacancy density with more active sides, while TiO2 nanoparticle was annealed in vacuum (∼10−1 torr) with oxide concentration conditions. From this finding, we propose that a thermal annealing process might serve as an approach for fabricating the photoanodes of TiO2-based materials consisting of much active photocatalyst