bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting the Annexin A1-FPR2/ALX pathway for host-directed therapy in dengue disease
Authors: Costa Vivian V , Sugimoto Michelle A , Hubner Josy , Bonilha Caio S , Queiroz-Junior Celso M , Gonçalves Pereira Marcela Helena , Chen Jianmin , Gobbetti Thomas , Libanio Rodrigues Gisele Olinto , Bambirra Jordana L. , Passos Ingredy , Machado Lopes Carla Elizabeth , Moreira Thaiane P. , Bonjour Kennedy , Melo Rossana C. N. , Oliveira Milton A. P. , Andrade Marcus Vinicius M. , Sousa Lirlândia Pires , Souza Danielle Gloria , Costa Santiago Helton da , Perretti Mauro , Teixeira Mauro Martins 
Published: November 2021

ABSTRACTHost immune responses contribute to dengue’s pathogenesis and severity, yet the possibility that failure in endogenous inflammation resolution pathways could characterise the disease has not been contemplated. The pro-resolving protein Annexin A1 (AnxA1) is known to counterbalance overexuberant inflammation and mast cell (MC) activation. We hypothesised that inadequate AnxA1 engagement underlies the cytokine storm and vascular pathologies associated with dengue disease. Levels of AnxA1 were examined in the plasma of dengue patients and infected mice. Immunocompetent, IFNα/βR-/-, AnxA1-/-and FPR2/ALX-/-mice were infected withDengue virus(DENV) and treated with the AnxA1 mimetic peptide Ac2-26for analysis. Additionally, the effect of Ac2-26on DENV-induced MC degranulation was assessedin vitroandin vivo. We observed that circulating levels of AnxA1 were reduced in dengue patients and DENV-infected mice. While the absence of AnxA1 or its receptor FPR2/ALX aggravated illness in infected mice, treatment with AnxA1 agonistic peptide attenuated disease manifestations. Both clinical outcomes were attributed to modulation of DENV-mediated viral load-independent MC degranulation. We have thereby identified that altered levels of the pro-resolving mediator AnxA1 are of pathological relevance in DENV infection, suggesting FPR2/ALX agonists as a therapeutic target for dengue disease.
Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungi
Authors: Ma Lay-Sun , Tsai Wei-Lun , Kalunke Raviraj M. , Xu Meng-Yun , Lin Yu-Han , Damei Florensia Ariani , Lee Hui-Chun 
Published: November 2021

AbstractAdapted plant pathogenic fungi deacetylate chitin to chitosan to avoid host perception and disarm the chitin-triggered plant immunity. Whether plants have evolved factors to counteract this fungal evasion mechanism in the plant-pathogen interface remains obscure. Here, we decipher the underlying mechanism of maize cysteine-rich receptor-like secreted proteins (CRRSPs)-AFP1, which exhibits mannose-binding dependent antifungal activity. AFP1 initials the action by binding to specific sites on the surface of yeast-like cells, filaments, and germinated spores of the biotrophic fungi Ustilago maydis. This could result in fungal cell growth and cell budding inhibition, delaying spore germination and subsequently reducing fungal viability in a mannose-binding dependence manner. The antifungal activity of AFP1 is conferred by its interaction with the PMT-dependent mannosylated chitin deacetylases (CDAs) and interfering with the conversion of chitin. Our finding that AFP1 targets CDAs from pathogenic fungi and nonpathogenic budding yeast suggests a potential application of the CRRSP in combating fungal diseases and reducing threats posed by the fungal kingdom.
Strontium isotopes reveal diverse life history variations, migration patterns, and habitat use for Broad Whitefish (Coregonus nasus) in Arctic, Alaska
Authors: Leppi Jason C. , Rinella Daniel J. , Wipfli Mark S. , Brown Randy J. , Spaleta Karen J. , Whitman Matthew S. 
Published: November 2021

AbstractConservation of Arctic fish species is challenging partly due to our limited ability to track fish through time and space, which constrains our understanding of life history diversity and lifelong habitat use. Broad Whitefish (Coregonus nasus) is an important subsistence species for Alaska’s Arctic Indigenous communities, yet little is known about life history diversity, migration patterns, and freshwater habitat use. Using laser ablation Sr isotope otolith microchemistry, we analyzed Colville River Broad Whitefish87Sr/86Sr chronologies (n = 61) to reconstruct movements and habitat use across the lives of individual fish. We found evidence of at least six life history types, including three anadromous types, one semi-anadromous type, and two nonanadromous types. Anadromous life history types comprised a large proportion of individuals sampled (collectively, 59%) and most of these (59%) migrated to sea between ages 0–2 and spent varying durations at sea. The semi-anadromous life history type comprised 28% of samples and entered marine habitat as larvae. Nonanadromous life history types comprised the remainder (collectively, 13%). Otolith87Sr/86Sr data from juvenile and adult freshwater stages suggest that habitat use changed in association with age, seasons, and life history strategies. This information on Broad Whitefish life histories and habitat use across time and space will help managers and conservation planners better understand the risks of anthropogenic impacts and help conserve this vital subsistence resource.
GapMind for Carbon Sources: Automated annotations of catabolic pathways
Authors: Price Morgan N. , Deutschbauer Adam M. , Arkin Adam P. 
Published: November 2021

AbstractGapMind for carbon sources is an automated tool for annotating catabolic pathways in bacterial and archaeal genomes. GapMind includes 62 compounds and identifies potential transporters and enzymes by their similarity to experimentally-characterized proteins. To improve GapMind’s coverage, we used high-throughput genetic data from 29 bacteria and systematically examined the gaps. We identified novel pathways or enzymes for the utilization of glucosamine, citrulline, myo-inositol, lactose, and phenylacetate, and we annotated 299 diverged enzymes and transporters. We also curated 125 proteins from published reports. For the 29 bacteria with genetic data, GapMind finds high-confidence paths for 85% of utilized carbon sources. In diverse bacteria and archaea, 38% of utilized carbon sources have high-confidence paths, which was improved from 27% by incorporating the fitness-based annotations and our curation. GapMind for carbon sources is available as a web server (http://papers.genomics.lbl.gov/carbon) and takes just 30 seconds for the typical genome.
Combinatorial expression of gamma-protocadherins regulates synaptic connectivity in the mouse neocortex
Authors: Zhu Yi-Jun , Deng Cai-Yun , Fan Liu , Wang Ya-Qian , Zhou Hui , Xu Hua-Tai 
Published: November 2021

AbstractIn the process of synaptic formation, neurons must not only adhere to specific principles when selecting synaptic partners but also possess mechanisms to avoid undesirable connections. Yet, the strategies employed to prevent unwarranted associations have remained largely unknown. In our study, we have identified the pivotal role of combinatorial clustered protocadherin gamma (γ-PCDH) expression in orchestrating synaptic connectivity in the mouse neocortex. Through 5-prime end single-cell sequencing, we unveiled the intricate combinatorial expression patterns of γ-PCDH variable isoforms within neocortical neurons. Furthermore, our whole-cell patch-clamp recordings demonstrated that as the similarity in this combinatorial pattern among neurons increased, their synaptic connectivity decreased. Our findings elucidate a sophisticated molecular mechanism governing the construction of neural networks in the mouse neocortex.
Prefrontal-amygdalar oscillations related to social interaction behavior in mice
Authors: Kuga Nahoko , Abe Reimi , Takano Kotomi , Ikegaya Yuji , Sasaki Takuya 
Published: November 2021

SUMMARYThe medial prefrontal cortex and amygdala are involved in the regulation of social behavior and associated with psychiatric diseases but their detailed neurophysiological mechanisms at a network level remain unclear. We recorded local field potentials (LFPs) from the dorsal medial PFC (dmPFC) and basolateral amygdala (BLA) while mice engaged on social behavior. We found that in wild-type mice, both the dmPFC and BLA increased 4–7 Hz oscillation power and decreased 30–60 Hz power when they needed to attend to another target mouse. In mouse models with reduced social interactions, dmPFC 4–7 Hz power further increased especially when they exhibited social avoidance behavior. In contrast, dmPFC and BLA decreased 4–7 Hz power when wild-type mice socially approached a target mouse. Frequency-specific optogenetic manipulations of replicating social approach-related LFP patterns restored social interaction behavior in socially deficient mice. These results demonstrate a neurophysiological substrate of the prefrontal cortex and amygdala related to social behavior and provide a unified pathophysiological understanding of neuronal population dynamics underlying social behavioral deficits.
Knowledge, attitude, utilisation and satisfaction of traditional Chinese medicine in Hong Kong
Authors: Ng Tommy K.C. , Lo Man Fung , Fong Ben Y.F. 
Published: November 2021

PurposeTraditional Chinese medicine (TCM) had a long history and has been widely practiced worldwide. TCM includes acupunctures, herbal medicine and chiropractic. However, limited studies examined the relationship between knowledge, attitude, utilisation and satisfaction of TCM among the Hong Kong general public. This study has developed a research model which aims to examine the relationship between knowledge, attitude, utilisation and satisfaction of TCM in Hong Kong by using partial least square structural equation model.Design/methodology/approachAn online-based questionnaire was distributed by using convenience sampling. The questionnaire consisted of five parts to collect the data regarding the knowledge, attitude, utilisation and satisfaction of TCM of respondents. The reflective measurement model and structural model were examined with SmartPLS 3.0 statistical software.FindingsA total of 131 respondents completed the survey, and all data were valid after data screening and cleaning. Around 60% of the participants received TCM information from their friends and family members, and 42% from the internet. Likewise, there is positive relationship from the knowledge of TCM to the utilisation, from the attitude to the utilisation and from the utilisation of TCM to the satisfaction. However, the positive relationship of knowledge regarding TCM and attitude is not proven. At-test and one-way analysis of variance showed no significant differences between gender and age groups on each measurement items.Originality/valueThis paper provides insights for researchers and policymakers to understand the significance of attitude and perception of the benefits of treatments in the use of TCM. The positive experience of TCM from other people is essential for enhancing the willingness to use TCM while education is also fundamental in promoting TCM to the public.
Historical research with Chinese multilateral historical marketing sources
Authors: Yan Tony , Hyman Michael R. 
Published: November 2021

Purpose
The rich primary and secondary data sources for studying historical Chinese marketing theory and practice are discussed. This paper aims to briefly address possible challenges (and their solutions) to using these sources.

Design/methodology/approach
A bibliographic review is used to analyze historical sources pertaining to Chinese marketing theory and practice.

Findings
Marketing scholars can draw from multiple but neglected and underused Chinese sources to glean important historical data reflecting pre-1949 Chinese marketing.

Research limitations/implications
Underused Chinese multilateral historical marketing materials are inalienable to extending historical marketing study. Many studies about marketing theory and practice are amenable to such materials.

Practical implications
By scrutinizing these materials, contemporary marketers can formulate parallel strategies from the repertoire of historical marketing strategies.

Originality/value
This is the first comprehensive survey of an invaluable non-Western source for historical research in marketing.
2E‐Decene‐4,6‐diyn‐1‐ol‐acetate inhibits osteoclastogenesis through mitogen‐activated protein kinase‐c‐Fos‐NFATc1 signalling pathways
Authors: Park Young Ran , Su Xiang‐Dong , Shrestha Saroj Kumar , Yang Seo Young , Soh Yunjo 
Published: November 2021

AbstractAn imbalance of osteoclasts and osteoblasts can result in a variety of bone‐related diseases, including osteoporosis. Thus, decreasing the activity of osteoclastic bone resorption is the main therapeutic method for treating osteoporosis. 2E‐Decene‐4, 6‐diyn‐1‐ol‐acetate (DDA) is a natural bioactive compound with anti‐inflammatory and anti‐cancer properties. However, its effects on osteoclastogenesis are unknown. Murine bone marrow‐derived macrophages (BMMs) or RAW264.7 cells were treated with DDA, followed by evaluation of cell viability, RANKL‐induced osteoclast differentiation, and pit formation assay. Effects of DDA on RANKL‐induced phosphorylation of MAPKs were assayed by western blot analysis. Expression of osteoclast‐specific genes was examined with reverse transcription‐PCR (RT‐PCR) and western blot analysis. In this study, DDA significantly inhibited RANKL‐induced osteoclast differentiation in RAW264.7 cells as well as in BMMs without cytotoxicity. DDA also strongly blocked the resorbing capacity of BMM on calcium phosphate‐coated plates. DDA inhibited RANKL‐induced phosphorylation of ERK, JNK and p38 MAPKs, as well as expression of c‐Fos and NFATc1, which are essential transcription factors for osteoclastogenesis. In addition, DDA decreased expression levels of osteoclastogenesis‐specific genes, including matrix metalloproteinase‐9 (MMP‐9), tartrate‐resistant acid phosphatase (TRAP), and receptor activator of NF‐κB (RANK) in RANKL‐induced RAW264.7 cells. Collectively, these findings indicated that DDA attenuates RANKL‐induced osteoclast formation by suppressing the MAPKs‐c‐Fos‐NFATc1 signalling pathway and osteoclast‐specific genes. These results indicate that DDA may be a potential candidate for bone diseases associated with abnormal osteoclast formation and function.
Extraction of phenolic compounds from the shells of pecan nuts with cytotoxic activity through apoptosis against the colon cancer cell line HT‐29
Authors: Ribas Lucas Emanuel , Baravalle María Eugenia , Gasser Fátima Belén , Renna María Sol , Addona Silvina , Ortega Hugo Héctor , Savino Graciela Hilda , Van de Velde Franco , Hein Gustavo Juan 
Published: November 2021

AbstractThe water extraction of phenolic compounds from two varieties (“Mahan” and “Marameck”) of pecan nutshells (Carya illinoinensis) without and with sonication, varying the solvent/solid ratio (S), the pH, and the refluxing time (t), was studied. Additionally, the in vitro cytotoxicity and the determination of the cell death mechanism of the extracts against the colon cancer cell line HT‐29 were investigated. The content of total phenolic compounds (TPC) of “Marameck” nutshells resulted higher than for the “Mahan” variety, and the pH increase resulted in higher TPC contents for both cultivars. The optimized conditions for TPC extraction without and with sonication resulted: S = 33 ml/g, pH = 12, and t = 9.6 min, and yielded ≈ 70 and 90 mg/g of TPC for “Mahan” and “Marameck” nutshells, respectively. The optimized extracts of pecan nutshells without sonication from both cultivars presented similar cytotoxicity against HT‐29 colon cancer cells (IC50 ≈ 50 µg/ml), higher than for sonicated extracts (IC50 ≈ 88 and 138 µg/ml for “Mahan” and “Marameck,” respectively). Cell death through apoptosis was the main mechanism of cell death induced by the nutshell extracts.Practical ApplicationThe extraction of phenolic compounds (TPC) from the residues of two varieties of pecan nutshells (“Mahan” and “Marameck”) was studied. An optimal combination of variables within the pH range that minimizes the solvent‐to‐solid ratio (S) and the time of refluxing (t), saving at the same time, water and energy, was set up. The phenolic compound extracts obtained from the residues of the pecan nuts exhibit cytotoxic effects against colon cancer cells and could be of interest as an alternative treatment of different types of cancer. Additionally, these extracts may be of importance to the food industry as they can be used as antioxidant agents in food formulation. Also, the high levels of anthocyanidins obtained from the pecan nut extracts after proanthocyanidins’ strong acid hydrolysis can be purified and employed as natural red dyes.
External validation of a machine learning classifier to identify unhealthy alcohol use in hospitalized patients
Authors: Lin Yiqi , Sharma Brihat , Thompson Hale M. , Boley Randy , Perticone Kathryn , Chhabra Neeraj , Afshar Majid , Karnik Niranjan S. 
Published: November 2021

AbstractBackground and AimsUnhealthy alcohol use (UAU) is one of the leading causes of global morbidity. A machine learning approach to alcohol screening could accelerate best practices when integrated into electronic health record (EHR) systems. This study aimed to validate externally a natural language processing (NLP) classifier developed at an independent medical center.DesignRetrospective cohort study.SettingThe site for validation was a midwestern United States tertiary‐care, urban medical center that has an inpatient structured universal screening model for unhealthy substance use and an active addiction consult service.Participants/CasesUnplanned admissions of adult patients between October 23, 2017 and December 31, 2019, with EHR documentation of manual alcohol screening were included in the cohort (n = 57 605).MeasurementsThe Alcohol Use Disorders Identification Test (AUDIT) served as the reference standard. AUDIT scores ≥5 for females and ≥8 for males served as cases for UAU. To examine error in manual screening or under‐reporting, a post hoc error analysis was conducted, reviewing discordance between the NLP classifier and AUDIT‐derived reference. All clinical notes excluding the manual screening and AUDIT documentation from the EHR were included in the NLP analysis.FindingsUsing clinical notes from the first 24 hours of each encounter, the NLP classifier demonstrated an area under the receiver operating characteristic curve (AUCROC) and precision‐recall area under the curve (PRAUC) of 0.91 (95% CI = 0.89–0.92) and 0.56 (95% CI = 0.53–0.60), respectively. At the optimal cut point of 0.5, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 0.66 (95% CI = 0.62–0.69), 0.98 (95% CI = 0.98–0.98), 0.35 (95% CI = 0.33–0.38), and 1.0 (95% CI = 1.0–1.0), respectively.ConclusionsExternal validation of a publicly available alcohol misuse classifier demonstrates adequate sensitivity and specificity for routine clinical use as an automated screening tool for identifying at‐risk patients.
Two quantitative trait loci are associated with recapping of Varroa destructor‐infested brood cells in Apis mellifera mellifera
Authors: Guichard M. , Dainat B. , Eynard S. , Vignal A. , Servin B. ,   the Beestrong Consortium, Neuditschko M. 
Published: November 2021

SummaryRecapping of Varroa destructor‐infested brood cells is a trait that has recently attracted interest in honey bee breeding to select mite‐resistant Apis mellifera colonies. To investigate the genetic architecture of this trait, we evaluated a sample of A. mellifera mellifera colonies (N = 155) from Switzerland and France and performed a genome‐wide association study, using a pool of 500 workers per colony for next‐generation sequencing. The results revealed that two QTL were significantly (P < 0.05) associated with recapping of V. destructor‐infested brood cells. The best‐associated QTL is located on chromosome 5 in a region previously found to be associated with grooming behaviour, a resistance trait against V. destructor, in A. mellifera and Apis cerana. The second best‐associated QTL is located on chromosome 4 in an intron of the Dscam gene, which is involved in neuronal wiring. Previous research demonstrated that genes involved in neuronal wiring are associated with recapping and varroa sensitive hygiene. Therefore, our study confirms the role of a gene region on chromosome 5 in social immunity and simultaneously provides novel insights into genetic interactions between common mite resistance traits in honey bees.
A Priori Bounds on Legislative Bargaining Agreements
Authors: Kalandrakis Tasos 
Published: November 2021

AbstractIn a workhorse model of legislative bargaining with spatial preferences, I establish easy to compute bounds on all equilibrium acceptable agreements, proposals, and outcomes. The approach constitutes a feasible method to incorporate equilibrium restrictions from the model in correlational and structural empirical studies of legislatures, avoiding the computation of actual equilibria. It also yields a number of theoretical insights on the centrality of equilibrium legislative decisions, the relation of such equilibrium outcomes with social choice solution sets, and the effect of changes on voting and proposal‐making rights. These theoretical results highlight the broad conclusion that the proper functioning of democratic institutions is highly contingent on other institutional features besides the assignment of voting rights.
Sleeping with the Enemy: Effective Representation under Dynamic Electoral Competition
Authors: Frey Anderson , López‐Moctezuma Gabriel , Montero Sergio 
Published: November 2021

AbstractElectoral coalitions between ideologically incompatible parties – among other unconventional electoral strategies – may seem to threaten effective representation, signaling a breakdown of programmatic politics. However, this perspective overlooks parties' and voters' dynamic considerations. We propose and estimate a model of dynamic electoral competition in which a short‐term ideology compromise, via an electoral coalition, offers opposition parties (and voters) the opportunity to remove an entrenched incumbent party from office, thus leveling the playing field in the future. This tradeoff provides a previously unrecognized rationale for coalition formation in elections. We take our model to data from Mexican municipal elections between 1995 and 2016 and show that coalitions between parties on opposite ends of the ideology spectrum have served as an instrument of democratic consolidation.