bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does a lower self-concept contribute to mental health disparities of diverse immigrant youth from middle childhood to late adolescence?
Authors: Busch J. , Claus C. , Schneider S. , Siefen R. G. 
Published: April 2021

Abstract
Background
Three out of ten children in Germany have immigrant backgrounds and this proportion is expected to further increase in subsequent years. While immigrant youth have been found more vulnerable to developing symptoms of depression and anxiety, the underlying mechanisms of how such disparities unfold during youth development are still understudied. Some previous research has found that immigrant youth are at risk of experiencing a less positive self-concept compared to non-immigrant youth. We investigated whether the self-concept mediates mental health disparities and explored variability in such associations from middle childhood to late adolescence.

Methods
Overall 1839 children and adolescents aged 6–21 years (M = 14.05 years, SD = 3.03, 49.8% female, n = 782 with immigrant status) participated in a cross-sectional self-report survey in classroom settings using scales from the Beck Youth Inventories II (Beck et al. in Beck Youth Inventories - Second Edition, Psychological Corporation, San Antonio, 2005) to assess self-concept and symptoms of depression and anxiety. Links between immigrant status, age, self-concept and symptom levels of depression as well as anxiety were examined using hierarchical regression and moderated mediation models.

Results
Immigrant youth reported higher symptom levels of depression and anxiety than their non-immigrant peers but did not differ in their self-concepts. Hypothesized moderated mediation models were not fully supported and self-concept neither mediated the link between immigrant status and depression nor immigrant status and anxiety. However, self-concept was a significant predictor for symptom levels of depression as well as anxiety, with stronger associations in adolescents.

Conclusions
Our study substantiates previous findings that immigrant youth in Germany have overall increased symptom levels of depression and anxiety compared to non-immigrant youth. Our study however does not support that immigrant youth have a more negative self-concept and that the self-concept mediates such internalizing mental health disparities. Findings match previous evidence that developing a positive attitude towards the self is linked to better mental health. Beyond that, our findings suggest that mental health interventions addressing the self-concept could be especially relevant when targeting adolescents. Further research is needed to deepen the understanding of the mediating processes between migration status and mental health variables.
Identification of selected genes associated with the SARS-CoV-2: a therapeutic approach and disease severity
Authors: Veerabathiran Ramakrishnan , Ragunath Barath , Kaviarasan Vaishak , Mohammed Vajagathali , Ahmed Shiek S. S. J. 
Published: April 2021

Abstract
Background
The ongoing pandemic of COVID-19 viruses takes its sole origin from the Wuhan Huanan seafood market, China. The first case was recorded as viral pneumonia and later became a worldwide pandemic (officially declared by WHO on March 11, 2020).

Main body
SARS-CoV-2 is an extremely infectious and transferrable virus that develops severe conditions like respiratory syndrome, high blood pressure and weakens the immune system. Coronavirus falls under the Coronaviridae family and Beta coronavirus genus. Affected individuals will encounter problems starting with fever followed by severe complications like SARS, ARDS, and many others. These SARS-CoV and MERS-CoV enter the host cells by the endosomal pathway, and about 16 non-structural proteins are involved in assembling the viral RNA synthesis complex. They possess a positive-sense single-stranded RNA, and about four major genes are mainly associated with the development of ASRD, SARS, and other respiratory problems.

Conclusion
Susceptibility of these four major genes such as ACE2, IL-2, 7 and 10, TNF, and VEGF is associated with COVID-19. This highlights the identification of the above-mentioned genes that can be used as potential biomarkers for early diagnosis and targeted drug delivery for treating the SARS-CoV-2 neurological symptoms and reducing inflammation in the brain.
A review of fire effects across South American ecosystems: the role of climate and time since fire
Authors: Giorgis Melisa A. , Zeballos Sebastian R. , Carbone Lucas , Zimmermann Heike , von Wehrden Henrik , Aguilar Ramiro , Ferreras Ana E. , Tecco Paula A. , Kowaljow Esteban , Barri Fernando , Gurvich Diego E. , Villagra Pablo , Jaureguiberry Pedro 
Published: April 2021

Abstract
Background
Fire is an important driver of ecosystem dynamics worldwide. However, knowledge on broad-scale patterns of ecosystem and organism responses to fires is still scarce. Through a systematic quantitative review of available studies across South America, we assessed fire effects on biodiversity and abundance of different organisms (i.e., plants, fungi, invertebrates, and vertebrates), plant fitness, and soil properties under four climate types, and time since the last fire (i.e., early and late post fire). We addressed: (1) What fire effects have been studied across South America? (2) What are the overall responses of biodiversity, abundance, fitness, and soil properties to fires? (3) How do climate and time since fire modulate those responses?

Results
We analyzed 160 articles reporting 1465 fire responses on paired burned and unburned conditions. We found no effect of fire on biodiversity or on invertebrate abundance, a negative effect on woody plant species and vertebrate abundance, and an increase in shrub fitness. Soil in burned areas had higher bulk density and pH, and lower organic matter and nitrogen. Fire effect was significantly more positive at early than at late post fire for plant fitness and for soil phosphorus and available nitrogen. Stronger negative effects in semiarid climate compared to humid warm climate suggest that higher temperatures and water availability allow a faster ecosystem recovery after fire.

Conclusions
Our review highlights the complexity of the climate–fire–vegetation feedback when assessing the response of soil properties and different organisms at various levels. The resilience observed in biodiversity may be expected considering the large number of fire-prone ecosystems in South America. The recovery of invertebrate abundance, the reduction of the vertebrate abundance, and the loss of nitrogen and organic matter coincide with the responses found in global reviews at early post-fire times. The strength of these responses was further influenced by climate type and post-fire time. Our synthesis provides the first broad-scale diagnosis of fire effects in South America, helping to visualize strengths, weaknesses, and gaps in fire research. It also brings much needed information for developing adequate land management in a continent where fire plays a prominent socio-ecological role.
Roles of bile acids in enteric virus replication
Authors: Kong Fanzhi , Saif Linda J. , Wang Qiuhong 
Published: April 2021

AbstractBile acids (BAs) are evolutionally conserved molecules synthesized in the liver from cholesterol to facilitating the absorption of fat-soluble nutrients. In the intestines, where enteric viruses replicate, BAs also act as signaling molecules that modulate various biological functions via activation of specific receptors and cell signaling pathways. To date, BAs present either pro-viral or anti-viral effects for the replication of enteric viruses in vivo and in vitro. In this review, we summarized current information on biosynthesis, transportation and metabolism of BAs and the role of BAs in replication of enteric caliciviruses, rotaviruses, and coronaviruses. We also discussed the application of BAs for cell culture adaptation of fastidious enteric caliciviruses and control of virus infection, which may provide novel insights into the development of antivirals and/or disinfectants for enteric viruses.
Improved imaging of gas hydrate reservoirs and their plumbing system using 2D elastic full-waveform inversion
Authors: Djeffal Adnan , Pecher Ingo A. , Singh Satish C. , Crutchley Gareth J. , Kaipio Jari 
Published: April 2021

Gas hydrates are ice-like crystalline materials that form under submarine environments of moderate pressure and low temperature. Another key factor to their formation is the abundance in gas supply from depth in addition to local biogenic gas. Detailed imaging and velocity analysis of the plumbing system of gas hydrates can provide confidence that amplitude anomalies in seismic data are related to gas hydrate accumulations. We have conducted 2D elastic full-waveform inversion (FWI) along a 14 km long segment of a 2D multichannel seismic profile to obtain a high-resolution velocity model of a hydrate system on the southern Hikurangi margin. We compare the FWI velocity model to previously published semblance- and tomography-based velocity models from the same data to explore how much more can be gained from the FWI. The FWI yielded a structurally more accurate velocity model that better delineated the low-velocity zone associated with free gas beneath the bottom simulating reflector (BSR) compared to the semblance- and tomography-based velocity models. Our results also find a lateral velocity inversion, that is, a narrow low-velocity zone surrounded by bands of higher velocities at a seaward-verging protothrust fault, which the two other methodologies failed to resolve. The FWI provides an improved lateral resolution making it an important tool when imaging the “plumbing” systems of gas hydrate reservoirs. In the southeastern limb of the anticline, our results find that the closely spaced landward-vergent protothrusts provide gas-charged fluids for hydrate formation above the BSR. Moreover, at the center of the anticline, our results find that a seaward-vergent protothrust fault appears to be acting as a conduit for gas-rich fluids into strata, although there is no accumulation of any significant hydrate above the BSR at the apex of the anticline. Our finding emphasizes the significance of densely spaced faults and fractures for providing gas for hydrate formation in the hydrate stability zone.
In vivoembryonic expression of laminin and its involvement in cell shape change in the sea urchinSphaerechinus granularis
Authors: McCarthy Robert A. , Burger Max M. 
Published: April 2021

ABSTRACTLaminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell shape change and are organized into an epithelium. Laminin is present on the basal surfaces of ectodermal cells and is absent or reduced on migrating primary mesenchyme cells. Microinjection of a monoclonal antibody directed against laminin induces a morphological change in cell shape and a deformation of the embryonic epithelium. Investigation of selected stages of live embryos suggests that the distribution of laminin may be heterogeneous within the basal lamina during early development. The results implicate laminin as a mediator of cell shape change during early morphogenesis.
Mapping the mouse X chromosome: possible symmetry in the location of a family of sequences on the mouse X and Y chromosomes
Authors: Avner Philip , Bishop Colin , Amar Laurence , Cambrou Jacques , Hatat Didier , Arnaud Danielle , Mattei Marie-Geneviève 
Published: April 2021

ABSTRACT
Major advances in our knowledge of the genetic organization of the mouse X chromosome have been obtained by the use of interspecific crosses involving Mus spretus-derived strains. This system has been used to study sequences detected by three probes 80Y/B, 302Y/B and 371Y/B isolated from a mouse Y-chromosome library which have been shown to recognize both male-female common and malefemale differential sequences. These patterns are due to the presence of a family of cross-reacting sequences on the mouse X and Y chromosomes. Detailed genetic analysis of the localization of the X-chromosome-specific sequences using both a somatic cell hybrid pane) and an interspecific mouse cross has revealed the presence of at least three discrete clusters of loci (Y-Y)A, (X-Y)B and (X-Y)C. Two of these clusters, (X-Y)B and (X-Y)C, lie distally on the mouse X chromosome, the other cluster (X-Y)A being situated close to the centromere. In situ hybridization shows a striking symmetry in the localization of the major sequences on both the X and Y chromosomes detected by these probes, hybridization being preferentially localized to a subcentromeric and subtelomeric region on each chromosome.
This striking localization symmetry between the X and Y chromosome sequences is discussed in terms of the extensive pairing of the X-Y chromosomes noted during meiosis.
Mapping the H-Y gene
Authors: Simpson Elizabeth , Chandler Phillip , Mclaren Anne , Goulmy Els , Disteche Christine M. , Page David C. , Ferguson-Smith Malcolm A. 
Published: April 2021

ABSTRACT
This paper uses cytotoxic and proliferative T cell clones specific for H-Y and restricted by MHC molecules to type mice and humans inheriting incomplete portions of the Y chromosome. The data have allowed us to map the H-Y antigen gene Hya in mouse to a position closely linked with, but separable from, Tdy on the Sxr fragment and thus presumably to a position of the normal mouse Y chromosome near the centromere. The human H-Y gene maps between deletion intervals 4B and 7, separate from TDF which is on interval 1. We are currently testing cells from a number of additional patients who have inherited different portions of the Y chromosome to pinpoint the mapping more closely. It is of interest that in mouse a Y-linked gene controlling spermatogenesis (Spy) maps near Hya on the Sxr fragment: they could be the same or closely linked genes. In man, a gene controlling spermatogenesis maps to Yq and the data so far do not exclude that it could be coincident with the H-Y gene.
Evidence for the presence of testicular tissue and Sxs antigen in the absence of Y-derived sequences
Authors: Wiberg Ulf H. , Scherer Gerd 
Published: April 2021

ABSTRACT
Eleven XX males and seven XX true hermaphrodites have been tested for the presence of Y-derived DNA sequences using six different probes. All eleven XX males were positive with at least one of the probes but none of the seven true hermaphrodites could be shown to possess any Y-DNA. Using a new sensitive test for serologically sex-specific (Sxs) antigen, we found that, despite their apparent lack of Y-DNA, the XX true hermaphrodites were positive for their expression of the Sxs antigen.
The use of specific DNA probes to analyse the Sxr mutation in the mouse
Authors: Bishop C. E. , Roberts C. , Michot J.-L. , Nagamine C. , Winking H. , Guénet J.-L. , Weith A. 
Published: April 2021

ABSTRACT
The mouse Y chromosome plays a fundamental role in the control of primary sex determination and fertility. Both genetic and molecular biological evidence has shown that much of the necessary information is contained in a minute piece of the Y (the Sxr region) which has arisen by a duplication of the pericentric region of the normal Y and the transposition of one copy to the distal pseudoautosomal region. The present article describes the isolation of random Y-chromosome probes and their use to investigate this Sxr region at the molecular level. Total mouse Y-chromosome libraries were constructed from flow-sorted material and a Sxr regional library after specific microdissection and cloning. Transcription has been detected in the testis using both Sxr-specific and non Sxr-located genomic probes taken from these libraries. In addition, we have been able to confirm the presence of an active steroid sulphatase gene on the mouse Y. This gene is located in the distal portion of the pseudoautosomal region and is tightly linked to Sxr. Finally, using an .S’xr-specific probe we can define multiple Y-chromosome haplotypes in the mouse showing that the region is evolving very rapidly.
The mammalian Y chromosome: molecular search for the sex-determining gene - summary and perspectives
Authors: Craig Ian , Levy Elaine , Fraser Neil 
Published: April 2021

ABSTRACT
Other presentations to this symposium have indicated that the search at the molecular level for the pivotal regulatory, or structural, gene responsible for determining the development of the undifferentiated gonad has been joined in earnest. It is also clear that genes on the Y chromosome are involved in processes other than primary determination of the testis. In this summary, we will review briefly ‘the molecular search for the sex-determining gene’ and consider the approaches that are available and the achievements that have been made in the areas relevant to an understanding of the roles and significance of other Y-located genes. The availability of molecular and physical mapping data also allow an examination of the evolutionary relationship of the mammalian X and Y chromosomes and a consideration of the possible homologies between the human and mouse Y chromosomes.
Cytocortical organization during natural and prolonged mitosis of mouse 8-cell blastomeres
Authors: Johnson Martin H. , Pickering Susan J. , Dhiman Arun , Radcliffe Graham S. , Maro Bernard 
Published: April 2021

Abstract
Late 8-cell blastomeres were harvested within the first 45 min after entering mitosis. Some mitotic cells were analysed within the ensuing 2 h for the organization of their surface in relation to their progress through mitosis. Whereas in most late interphase cells microvilli were restricted to a discrete polar region, in mitotic cells at all stages from early metaphase to immediately postcytokinesis microvilli were found to be present over more of the cell surface. Other mitotic cells were placed in nocodazole to arrest them in M-phase for up to 10 h. They were found to show an even more extensive distribution of microvilli over the whole surface, the longer periods of incubation yielding more extended coverage such that many cells no longer appeared to have any residual surface polarity. Removal from nocodazole at all time points from 1 to 10 h resulted in most cells completing mitosis to yield pairs of cells which, in most cases, resembled pairs derived from nonarrested blastomeres and in which a defined polar area of microvilli was restored. However, the percentage of differentiative divisions decreased after 6h arrest. If, instead of removing cells from nocodazole, they were placed in both nocodazole and cytochalasin D (CCD) for periods of up to 3h, most microvilli retracted to reveal a tight polar zone of CCD-resistant microvilli. This result suggests that a heterogeneity of cytocortical organization may still exist within the arrested mitotic cell. We propose a model to explain the origin of this heterogeneity of organization and its relationship to the generation of cell diversity.
The albino deletion complex and early postimplantation survival in the mouse
Authors: Niswander Lee , Yee Della , Rinchik Eugene M. , Russell Liane B. , Magnuson Terry 
Published: April 2021

Abstract
The albino deletion complex in the mouse represents 37 overlapping chromosomal deflciencies that have been arranged into at least twelve complementation groups. Many of the deletions cover regions of chromosome 7 that contain genes necessary for early embryonic development. The work reported here concentrates on two of these deletions (c6H, cIIDSD), both of which were known to be lethal around the time of gastrulation when homozygous. A detailed embryological analysis has revealed distinct differences in the lethal phenotype associated with the c6H and cIIDSD deletions. c6H homozygous embryos are grossly abnormal at day 7·5 of gestation, whereas C11DSD homozygous embryos appear abnormal at day 8·5 of gestation. There is no development of the extraembryonic ectoderm in homozygotes, whereas extensive development of this tissue type occurs in c11DSD homozygotes. The visceral endoderm is abnormally shaped and the parietal endoderm appears to be overproduced in c6H homozygotes; these structures are not affected in c11DSD homozygotes. The embryonic ectoderm is runted in both types of embryo and it is not possible to obtain homozygous embryo-derived stem-cell lines for either deletion. Mesoderm formation occurs in the C11DSD but not in the c6H homozygotes. The c11DSD deletion chromosome complements the c6H chromosome in that the lethal phenotype of the compound heterozygote is similar to that of the c11DSD homozygote. These results suggest that a gene(s) necessary for normal development of the extraembry-onic ectoderm is present in the c11080 but deficient in the c6H deletion chromosome.
The submitted manuscript has been co-authored by a contractor of the US Government under contract No. DE-AC05-840R21400. Accordingly, the US Government retains a nonexclusive, royalty-free licence to publish or reproduce the published form of the contribution, or allow others to do so, for US Government Purposes.
‘Early’ mammalian myoblasts are resistant to phorbol ester-induced block of differentiation
Authors: Cossu G. , Ranaldi G. , Senni M. I. , Molinaro M. , Vivarelli E. 
Published: April 2021

Abstract
Mesenchymal cells were isolated from somites and limbs of mouse embryos at different developmental stages. When grown in tissue culture, some of the cells underwent muscle differentiation as indicated by synthesis of sarcomeric myosin, acetylcholine receptor and, in the case of limb cells, fusion into multinucleated myotubes.
When the tumour promoter 12-O-tetradecanoyl phorbol 13-acetate (TPA) was added to these cultures, it caused differential effects, depending upon the age of the embryo from which cells were isolated. In cultures of somites or limb bud from embryos up to 12 days post coitum, TPA did not interfere with the appearance of differentiated muscle cells. When TPA was added to cultures from older embryos, it inhibited muscle differentiation with an efficiency which increased with the age of the embryo, reaching about 90 % inhibition at 15 days.
After this period, a new population of myogenic cells appeared in the limb, which were able to differentiate in the presence of TPA and represented the great majority of myoblasts after day 18 of embryonic development.
The simplest interpretation of these data can be based on the existence of three major classes of myogenic cell precursors, which appear sequentially during muscle histogenesis: ‘early’ myoblasts, which appear resistant to tumour promoters; ‘late’ myoblasts, whose differentiation is inhibited by tumour promoters and ‘satellite’ cells which, like early myoblasts, show no sensitivity to TPA.
Lithium dorsalizes but also mechanically disrupts gastrulation ofXenopus laevis
Authors: Regen Chester M. , Steinhardt Richard A. 
Published: April 2021

AbstractThe discovery that lithium treatment at blastula stages can induce axis formation suggested that it might act by respecifying the cytoplasmic rearrangement-generated dorsoventral pattern, so that ventral cells behave like their dorsal counterparts. We have studied the effects of Li+ treatment on the spatial layout of the cell-group movements of gastrulation to see whether this is the case. We find that involution of the chordamesoderm and associated archenteron roof is retarded by Li+, an effect which does not suggest dorsal respecification. However, in both migration of the leading edge mesoderm and convergent extension of the marginal zone, ventral regions clearly do show dorsal-type movement. Because of this, and because of examples where disruption of involution and effects on axis differentiation do not correlate, we propose that failure of involution represents a distinct effect of Li+ involving disruption of mechanical relationships at the blastopore. Thus archenteron formation poorly reflects the dorsoventral pattern. Extension of sandwich explants of the ventral marginal zone is proposed as a reliable quantitative assay for alterations to the dorsoventral pattern.