bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Uniportal video-assisted thoracic surgery of bilateral pulmonary nodules
Authors: Lin Shengcheng , Yang Chenglin , Guo Xiaotong , Xu Yafei , Wang Lixu , Wang Zhe , Yu Xin , Wang Chunguang , Yu Zhentao 
Published: March 2021

Abstract
Background
Surgical resection is an appropriate treatment option for synchronous bilateral pulmonary nodules with ground-glass opacities. The applicability of simultaneous uniportal video-assisted thoracic surgery is not fully understood. We evaluated the feasibility and safety of performing such surgeries at our hospital.

Methods
Clinical data of 35 patients who underwent simultaneous bilateral pulmonary resection with uniportal video-assisted thoracic surgery at our hospital were reviewed retrospectively.

Results
Simultaneous bilateral pulmonary resection with uniportal video-assisted thoracic surgery was performed for 35 patients (15 men, 20 women); 97 nodules were operated on, and the average nodule diameter was 11.4 mm (range, 1–38 mm). Computerized tomography showed that most nodules had ground-glass opacity (52/97, 53.6%); solid nodules (24/97, 24.7%) and nodules with mixed ground-glass opacity (21/97, 21.7%) were noted. Surgical resection included lobar-sublobar resection (11/35, 31.4%) and sublobar-sublobar resection (24/35, 68.6%). Wound infection and postoperative 30-day mortality were not observed. Pneumonia was the major postoperative complication, with a higher incidence in the lobar-sublobar group (6/10, 60%) than in the sublobar-sublobar group (4/25, 16%; P = 0.016). Pneumonia did not correlate with operative time (mean, 262.3 ± 108.1 vs. 261.9 ± 87.5 min, P = 0.991), duration of chest drainage (mean, 7.0 ± 4.0 vs 5.4 ± 2.1 days, P = 0.124), and postoperative hospital stay (mean, 10.2 ± 3.6 vs 10.2 ± 6.4 days, P = 0.978). The mean follow-up time was 8 (range, 3–22) months. Recurrence of primary lung cancer or mortality was not noted at the final follow-up.

Conclusions
Simultaneous bilateral pulmonary resection with uniportal video-assisted thoracic surgery is feasible and safe for appropriate patients. Simultaneous lobar-sublobar pulmonary resection for bilateral nodules can increase the risk of developing pneumonia.
Prenatal diagnosis of 7 cases with uniparental disomy by utilization of single nucleotide polymorphism array
Authors: Zhou Lili , Zheng Zhaoke , Xu Yunzhi , Lv Xiaoxiao , Xu Chenyang , Xu Xueqin 
Published: March 2021

Abstract
Background
The phenotypes of uniparental disomy (UPD) are variable, which may either have no clinical impact, lead to clinical signs and symptoms. Molecular analysis is essential for making a correct diagnosis. This study involved a retrospective analysis of 4512 prenatal diagnosis samples and explored the molecular characteristics and prenatal phenotypes of UPD using a single nucleotide polymorphism (SNP) array.

Results
Out of the 4512 samples, a total of seven cases of UPD were detected with an overall frequency of 0.16%. Among the seven cases of UPD, two cases are associated with chromosomal aberrations (2/7), four cases (4/7) had abnormal ultrasonographic findings. One case presented with iso-UPD (14), and two case presented with mixed hetero/iso-UPD (15), which were confirmed by Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) as maternal UPD (15) associated with Prader-Willi syndrome (PWS). Four cases had iso-UPD for chromosome 1, 3, 14, and 16, respectively; this is consistent with the monosomy rescue mechanism. Another three cases presented with mixed hetero/isodisomy were consistent with a trisomy rescue mechanism.

Conclusion
The prenatal phenotypes of UPD are variable and molecular analysis is essential for making a correct diagnosis and genetic counselling of UPD. The SNP array is a useful genetic test in prenatal diagnosis cases with UPD.
DNA methylation biomarker for cumulative lead exposure is associated with Parkinson’s disease
Authors: Paul Kimberly C. , Horvath Steve , Del Rosario Irish , Bronstein Jeff M. , Ritz Beate 
Published: March 2021

AbstractLead, a known neurotoxicant, has previously received attention in Parkinson’s disease (PD) research, but epidemiologic studies have been limited in sample size and findings are equivocal. We generated two methylation-based biomarkers for cumulative tibia and patella bone-measured lead exposure in 1528 PD patients and 1169 controls. PD status was associated with increased levels of the DNAm biomarker for tibia-lead levels. We estimated a meta-OR for PD of 1.89 per unit DNAm tibia-lead increase (95% CI 1.59, 2.24; p = 8.1E−13). The current study supports the notion that chronic and long-term lead exposure tracked via DNAm may contribute to PD pathogenesis.
Birthweight DNA methylation signatures in infant saliva
Authors: Moccia Chiara , Popovic Maja , Isaevska Elena , Fiano Valentina , Trevisan Morena , Rusconi Franca , Polidoro Silvia , Richiardi Lorenzo 
Published: March 2021

Abstract
Background
Low birthweight has been repeatedly associated with long-term adverse health outcomes and many non-communicable diseases. Our aim was to look-up cord blood birthweight-associated CpG sites identified by the PACE Consortium in infant saliva, and to explore saliva-specific DNA methylation signatures of birthweight.

Methods
DNA methylation was assessed using Infinium HumanMethylation450K array in 135 saliva samples collected from children of the NINFEA birth cohort at an average age of 10.8 (range 7–17) months. The association analyses between birthweight and DNA methylation variations were carried out using robust linear regression models both in the exploratory EWAS analyses and in the look-up of the PACE findings in infant saliva.

Results
None of the cord blood birthweight-associated CpGs identified by the PACE Consortium was associated with birthweight when analysed in infant saliva. In saliva EWAS analyses, considering a false discovery rate p-values < 0.05, birthweight as continuous variable was associated with DNA methylation in 44 CpG sites; being born small for gestational age (SGA, lower 10th percentile of birthweight for gestational age according to WHO reference charts) was associated with DNA methylation in 44 CpGs, with only one overlapping CpG between the two analyses. Despite no overlap with PACE results at the CpG level, two of the top saliva birthweight CpGs mapped at genes associated with birthweight with the same direction of the effect also in the PACE Consortium (MACROD1 and RPTOR).

Conclusion
Our study provides an indication of the birthweight and SGA epigenetic salivary signatures in children around 10 months of age. DNA methylation signatures in cord blood may not be comparable with saliva DNA methylation signatures at about 10 months of age, suggesting that the birthweight epigenetic marks are likely time and tissue specific.
Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids
Authors: Chen Wenyi , Zhu Jiaqi , Lin Feiyan , Xu Yanping , Feng Bing , Feng Xudong , Sheng Xinyu , Shi Xiaowei , Pan Qiaoling , Yang Jinfeng , Yu Jiong , Li Lanjuan , Cao Hongcui 
Published: March 2021

Abstract
Background
Cholangiocyte senescence is an important pathological process in diseases such as primary sclerosing cholangitis (PSC) and primary biliary cirrhosis (PBC). Stem cell/induced pluripotent stem cell-derived exosomes have shown anti-senescence effects in various diseases. We applied novel organoid culture technology to establish and characterize cholangiocyte organoids (cholangioids) with oxidative stress-induced senescence and then investigated whether human placenta mesenchymal stem cell (hPMSC)-derived exosomes exerted a protective effect in senescent cholangioids.

Methods
We identified the growth characteristics of cholangioids by light microscopy and confocal microscopy. Exosomes were introduced concurrently with H2O2 into the cholangioids. Using immunohistochemistry and immunofluorescence staining analyses, we assessed the expression patterns of the senescence markers p16INK4a, p21WAF1/Cip1, and senescence-associated β-galactosidase (SA-β-gal) and then characterized the mRNA and protein expression levels of chemokines and senescence-associated secretory phenotype (SASP) components.

Results
Well-established cholangioids expressed cholangiocyte-specific markers. Oxidative stress-induced senescence enhanced the expression of the senescence-associated proteins p16INK4a, p21WAF1/Cip1, and SA-β-gal in senescent cholangioids compared with the control group. Treatment with hPMSC-derived exosomes delayed the cholangioid aging progress and reduced the levels of SASP components (i.e., interleukin-6 and chemokine CC ligand 2).

Conclusions
Senescent organoids are a potential novel model for better understanding senescence progression in cholangiocytes. hPMSC-derived exosomes exert protective effects against senescent cholangioids under oxidative stress-induced injury by delaying aging and reducing SASP components, which might have therapeutic potential for PSC or PBC.
Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s disease
Authors: Friedrich I. , Reimann K. , Jankuhn S. , Kirilina E. , Stieler J. , Sonntag M. , Meijer J. , Weiskopf N. , Reinert T. , Arendt T. , Morawski M. 
Published: March 2021

AbstractIron is essential for neurons and glial cells, playing key roles in neurotransmitter synthesis, energy production and myelination. In contrast, high concentrations of free iron can be detrimental and contribute to neurodegeneration, through promotion of oxidative stress. Particularly in Parkinson’s disease (PD) changes in iron concentrations in the substantia nigra (SN) was suggested to play a key role in degeneration of dopaminergic neurons in nigrosome 1. However, the cellular iron pathways and the mechanisms of the pathogenic role of iron in PD are not well understood, mainly due to the lack of quantitative analytical techniques for iron quantification with subcellular resolution. Here, we quantified cellular iron concentrations and subcellular iron distributions in dopaminergic neurons and different types of glial cells in the SN both in brains of PD patients and in non-neurodegenerative control brains (Co). To this end, we combined spatially resolved quantitative element mapping usingmicroparticleinducedX-rayemission (µPIXE) with nickel-enhanced immunocytochemical detection of cell type-specific antigens allowing to allocate element-related signals to specific cell types. Distinct patterns of iron accumulation were observed across different cell populations. In the control (Co) SNc, oligodendroglial and astroglial cells hold the highest cellular iron concentration whereas in PD, the iron concentration was increased in most cell types in the substantia nigra except for astroglial cells and ferritin-positive oligodendroglial cells. While iron levels in astroglial cells remain unchanged, ferritin in oligodendroglial cells seems to be depleted by almost half in PD. The highest cellular iron levels in neurons were located in the cytoplasm, which might increase the source of non-chelated Fe3+, implicating a critical increase in the labile iron pool. Indeed, neuromelanin is characterised by a significantly higher loading of iron including most probable the occupancy of low-affinity iron binding sites. Quantitative trace element analysis is essential to characterise iron in oxidative processes in PD. The quantification of iron provides deeper insights into changes of cellular iron levels in PD and may contribute to the research in iron-chelating disease-modifying drugs.
The descriptive epidemiology of brand-specific gun ownership in the US: results from the 2019 National Lawful Use of Guns Survey
Authors: Siegel Michael , Dunn Devon , Shareef Faizah , Neufeld Miriam , Boine Claire 
Published: March 2021

Abstract
Background
No previous study has identified the specific brands of guns owned by gun owners. This study aimed to: (1) ascertain and describe patterns of brand- and model-specific gun ownership among US gun owners; and (2) investigate the relationship between gun owners’ brand and model preferences and their attitudes towards common firearm violence prevention policies.

Methods
Using a national, pre-recruited internet panel of US adults in 2019, we surveyed gun owners (N = 2086) to ascertain their opinions regarding firearm violence prevention policies and to assess the brands and models of guns that they owned.

Results
Brand-specific gun ownership was highly concentrated and was dominated by three pistol brands, two revolver brands, three rifle brands, and three shotgun brands. There was wide variation in policy attitudes among owners of different gun brands, but little variation across owners of different gun types (i.e., pistols, rifles, revolvers, shotguns). We were able to identify the specific gun models owned by 1218 (59.4%) of the gun owners. Based on the classification of these gun models into three types we categorized the gun ownership pattern of the sample as 33.4% recreational, 45.5% self-defense, and 21.1% tactical. There were marked differences in support for firearm-related policies among the three groups, with support generally highest among the Recreation group and lowest among the Tactical group.

Conclusion
We conclude that gun brands and models are strong predictors of a gun owner’s attitudes regarding firearm-related policies. This information could help public health practitioners develop segment-specific communications that will appeal to each group in order to more effectively engage gun owners in firearm violence prevention.
Patterns of alcohol and drug utilization in trauma patients during the COVID-19 pandemic at six trauma centers
Authors: McGraw Constance , Salottolo Kristin , Carrick Matthew , Lieser Mark , Madayag Robert , Berg Gina , Banton Kaysie , Hamilton David , Bar-Or David 
Published: March 2021

Abstract
Background
Since the national stay-at-home order for COVID-19 was implemented, clinicians and public health authorities worldwide have expressed growing concern about the potential repercussions of drug and alcohol use due to social restrictions. We explored the impact of the national stay-at-home orders on alcohol or drug use and screenings among trauma admissions.

Methods
This was a retrospective cohort study at six Level I trauma centers across four states. Patients admitted during the period after the onset of the COVID-19 restrictions (defined as March 16, 2020-May 31, 2020) were compared with those admitted during the same time period in 2019. We compared 1) rate of urine drug screens and blood alcohol screens; 2) rate of positivity for drugs or alcohol (blood alcohol concentration ≥ 10 mg/dL); 3) characteristics of patients who were positive for drug or alcohol, by period using chi-squared tests or Fisher’s exact tests, as appropriate. Two-tailed tests with an alpha of p < 0.05 was used on all tests.

Results
There were 4762 trauma admissions across the study period; 2602 (55%) in 2019 and 2160 (45%) in 2020. From 2019 to 2020, there were statistically significant increases in alcohol screens (34% vs. 37%, p = 0.03) and drug screens (21% vs. 26%, p < 0.001). Overall, the rate of alcohol positive patients significantly increased from 2019 to 2020 (32% vs. 39%, p = 0.007), while the rate of drug positive patients was unchanged (57% vs. 52%, p = 0.13). Of the 1025 (22%) patients who were positive for alcohol or drugs, there were significant increases in a history of alcoholism (41% vs. 26%, p < 0.001), and substance abuse (11% vs. 23%, p < 0.001) in the 2020 period. No other statistically significant differences were identified among alcohol or drug positive patients during COVID-19 compared to the same period in 2019.

Conclusions
Our first wave of COVID-19 data suggests that trauma centers were admitting significantly more patients who were alcohol positive, as well those with substance use disorders, potentially due to the impact of social restrictions and guidelines. Further longitudinal research is warranted to assess the alcohol and drug positive rates of trauma patients over the COVID-19 pandemic.
Two epochal turns of inequality, their significance, and their dynamics
Authors: Therborn Göran 
Published: March 2021

AbstractAt the end of the twentieth century, two historical turns of economic inequality happened. Among the developed countries of the Global North, the secular trend of decreasing intra-national inequality turned into its opposite. At about the same time, the long period of global inequality began to bend down, among households as well as among nations, a turn less noticed but more significant than the reduction of extreme poverty in the South. The foundation of the former turn was the beginning of de-industrialization in the North, and the coming of a post-industrial society, very different from the one predicted. The paper analyzes the trigger of the turn and the central dynamics of the new inequality in the rich North, financialization, and the digital revolution. It then tries to answer two questions about the global turn: Was the decline of global inequality causally connected to the increase of Northern intra-national inequality? Will there be a development of industrial societies in the South? The answer to both is no. What lies ahead is more likely a global convergence of intra-national unequalization, albeit with both different and similar dynamics, as the decline of extreme poverty in the South is leading to inequality increases comparable to those of the North. Post-industrialism has no egalitarian dialectic like that of industrial capitalism, but the dynamics of the twenty-first century inequality are likely to be confronted not only with popular protest movements but also with an emergent scholarly and intellectual Egalitarian Enlightenment.
How do borders influence migration? Insights from open and closed border regimes in the three Guianas
Authors: Vezzoli Simona 
Published: March 2021

AbstractThe ways border regimes affect migration patterns remain ambiguous. Closed borders may constrain migration but also encourage migrants to pursue alternative migration channels and destinations. While open borders may be associated with higher migration, oftentimes they promote circulation and return. To clarify how different border regimes influence migration patterns, this article examines the impact of open and closed border regimes on migration outcomes in Guyana, Suriname and French Guiana in the 1950s–1980s period, when all three gained independence or non-sovereign status and colonial ties were strong. The article proposes a conceptual schematic model that can accommodate varied post-colonial political and border regime transitions and explains changes in the timing, destination and composition of migration. The analysis finds that, counterintuitively, closed borders can lead to high emigration while open borders can encourage people to stay. The proposed model also illustrates the relevance of three dimensions of time: thehistorical juncture, thesequence of changeand thetime span. In sum, rather than preventing migration, border regimes yield important effects that lead to migration diversification.
How migrants manifest their transnational identity through online social networks: comparative findings from a case of Koreans in Germany
Authors: Park Sunyoung , Gerrits Lasse 
Published: March 2021

AbstractAlthough migration has long been an imperative topic in social sciences, there are still needs of study on migrants’ unique and dynamic transnational identity, which heavily influences the social integration in the host society. In Online Social Network (OSN), where the contemporary migrants actively communicate and share their stories the most, different challenges against migrants’ belonging and identity and how they cope or reconcile may evidently exist. This paper aims to scrutinise how migrants are manifesting their belonging and identity via different technological types of online social networks, to understand the relations between online social networks and migrants’ multi-faceted transnational identity. The research introduces a comparative case study on an online social movement led by Koreans in Germany via their online communities, triggered by a German TV advertisement considered as stereotyping East Asians given by white supremacy’s point of view. Starting with virtual ethnography on three OSNs representing each of internet generations (Web 1.0 ~ Web 3.0), two-step Qualitative Data Analysis is carried out to examine how Korean migrants manifest their belonging and identity via their views on “who we are” and “who are others”. The analysis reveals how Korean migrants’ transnational identities differ by their expectation on the audience and the members in each online social network, which indicates that the distinctive features of the online platform may encourage or discourage them in shaping transnational identity as a group identity. The paper concludes with the two main emphases: first, current OSNs comprising different generational technologies play a significant role in understanding the migrants’ dynamic social values, and particularly, transnational identities. Second, the dynamics of migrants’ transnational identity engages diverse social and situational contexts. (keywords: transnational identity, migrants’ online social networks, stereotyping migrants, technological evolution of online social network).
Tracheoinnominate fistula: acute bleeding and hypovolemic shock due to a trachea-innominate artery fistula after long-term tracheostomy, treated with a stent-graft
Authors: Khanafer Ali , Hellstern Victoria , Meißner Helfried , Harmening Christoph , Schneider Klaus , Henkes Hans 
Published: March 2021

Abstract
Background
A tracheo-innominate fistula is a rare but life-threatening complication of tracheostomy and has a mortality rate of 100% without therapy. The underlying cause is an acquired fistula between the brachiocephalic trunk and the trachea, induced by a tracheostomy cannula’s mechanical impact.

Case presentation
A 25-year-old female was admitted with pulsatile bleeding from a tracheostomy. The cause of the bleeding was a tracheo-innominate artery fistula, which was difficult to recognize. Said fistula was treated with implantation of a self-expanding stent-graft. The bleeding stopped immediately after the implantation of the stent-graft. Dual antiplatelet medication with aspirin IV and ticagrelor PO, bridged with a bolus of eptifibatide IV, was started right after the stent deployment.

Conclusions
Endovascular self-expanding stent-graft implantation is a viable treatment option for tracheo-innominate artery fistulae, especially in hemorrhagic emergencies.