bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Rights: Integrity of the Russian and International Law, Competition of Courts Decisions
Authors: Lazutin L. A. , Likhachev M. A. 
Published: October 2021

INTRODUCTION. The amendments to the Russian Constitution 2020 challenged de novo the international law prevalence and led the Russia’s way to find it own perception of international law. Although the amendments did not introduce drastically substantive modifications of the international law modus operandi in the national legal system nevertheless they shifted the constitutional focus. The former one was built on the presumption of the juridical consistency of the constitutional order and Russia’s international commitments. Today there’s the a priori allegation of possible conflicts between requirements of the Constitution and judgment of international courts.MATERIALS AND METHODS. The paper comprises short historical analysis of the internationally meaningful rules of the Constitution in its comparison to the current legal situation in Russia. Rather superficial but illustrative juridical overview of the relevant constitutional provisions with their domestic legal counterparts demonstrates the significance and practical efficiency of the concomitant interpretation of the constitutional rules and Russia’s international obligations.RESEARCH RESULTS. Such a shift paradigmatically is still pending new interpretation of the constitutional fundamentals. At least they need different construction to be concomitant to the refusal mechanism (as regards international judgments). Still unchanged verbatim the constitutional fundamentals provide for proliferated mechanism of the human rights protection under international las with in domestic order and still require the concordant interpretation of the international commitments and constitutional rules.DISCUSSION AND CONCLUSIONS. The modified constitutional landscape shifted drastically the international law priority in the Russian legal system. Although the international law leaves to the State’s choice to determine internally the status of its international commitments the constitutional fundamentals (left untouched verbatim) still require international law priority. The constitutionally enclosed human right protection mechanism emphasizes such priority.
Antiviral and antiretroviral drug shortages amidst COVID-19: How Africa is struggling
Authors: Rackimuthu Sudhan , Kazmi Zohra , Elmahi Osman Kamal Osman , Hunain Reem , Ghazi Behram Khan , Zaidi Zain Ali , Costa Ana Carla dos Santos , Ahmad Shoaib , Essar Mohammad Yasir 
Published: October 2021

Antiviral drugs are of paramount importance in the accomplishment of the vision of zero new cases of COVID-19 globally, through sustainable retaliation against viral diseases. However, several challenges currently exist in Africa which include insufficient infrastructure, deteriorating health systems, and rising costs of healthcare delivery with concomitant rising inequity with regards to access to health services amid the COVID-19 pandemic. The pandemic itself has stimulated an increased use of phytotherapy in Africa as a result of essential drug shortages that have been attributed to a plethora of contributing factors such as travel restrictions, reduced per capita income as well as increased expenditure on transport. As a result, the paucity of antiviral along with antiretroviral drugs used to combat COVID-19 as well as several other endemic viral diseases in Africa has created a worrisome state. This article therefore discusses and aims to underscore the causes, effects, and implications of antiviral and antiretroviral shortages amid COVID-19 in Africa.
THE EFFECT OF SOAKING SALTS CONCENTRATION TO CANDIED GARLIC (Sativum allium L.)
Authors: Titaley Naning Restiani , Herawati Maria Marina 
Published: October 2021

Garlic has a bitter taste, which makes people reluctant to eat one whole garlic clove. The salt soaking technique used to reduce the bitter taste of the garlic, and the garlic processed into wet candied garlic, so it became more edible than not process. The process of candied garlic could preserve the durability level, which allows it to be stored longer at low temperatures. The research materials used were salt and sugar. The purpose of the research was to know the effect of salt concentration on reducing bitterness and the right concentration to reduce the bitter taste of garlic. This research used the RAK method, and DMRT (Duncan’s Multiple Range Test), and the samples of salt were 3%; 3.5%; 4%; 4.5%. The test included chemical tests (water content and total microbes) and organoleptic tests (taste, aroma, and texture). The water content of the product showed the result, which was not significantly different, and the total microbes test showed no microorganism was found or grew on the product. The organoleptic test of taste was obtained on 3.5% salt, with an average of 2.75% (likes), while the aroma and texture did not show a significant difference in the result. The aroma and texture data obtained on 2.90 (likes) and 2.40 (likes) with 3.5% salt. Keywords: Candied; Garlic; Salt; Soaking; Sugar.
Total Polyphenol, Rehydration Ratio, and Liquor Color of Different Grade Green Tea
Authors: Prawira-Atmaja M Iqbal , Azhary Beauty , Harianto Sugeng , Maulana Hilman , Shabri Shabri , Rohdiana Dadan 
Published: October 2021

Tea is a non-alcoholic beverage that is widely consumed after water with potential health benefits. In general, tea processing divided into three types, non-enzymatic oxidation process (green tea), semi-oxidation (oolong tea), and with enzymatic oxidation (black tea). The sorting and grading is a stage in controlling the quality of green tea. This study aims to determine the characteristics of different grades of green tea on the total polyphenols, liquor color, and rehydration ratio. The results showed that the pekoe/pecco grade had the highest total polyphenol and rehydration ratio compared to other grades. Pekoe grade has a greenish yellow and brighter liquor color. Application of good agriculture practice (GAP) and plucking of fresh tea leaves should be considered to obtain more grade pekoe in the green tea processing.
Mini Review: Hypocholesterolemic Potential of Unsaponifiable Matter from Palm Fatty Acid Distillate
Authors: Wulansari Angela , Estiasih Teti , Yunianta Yunianta 
Published: October 2021

Palm fruit as raw material of palm oil is the main commodity of Indonesian agriculture. Palm fatty acid distillate (PFAD) is a by-product of palm oil refinery process in deodorization stage. PFAD contained bioactive compounds such as vitamin E, phytosterols, and squalene. Vitamin E, phytosterols, and squalene have knows for its hypocholesterolemic effect and cardio protector. Bioactive compounds of PFAD can be separated from its fatty acid using saponification reaction. Saponification of PFAD produced unsaponifiable matter (USM) which was rich in bioactive compounds. Food fortification is aimed to enhance the nutrition quality of food for specific reason. The fortificant can be macronutrient or micronutrient. USM of PFAD potentially become food product fortificant which will give hypocholesterolemic effect. This review is discussed about hypocholesterolemic effect of each bioactive compounds in USM of PFAD and the potential of USM as food fortificant espsecially for food with hypocholesterolemic effect.
DIFFERENTIAL EXPRESSION OF MICRORNA IN GENERALIZED ANXIETY DISORDER AND ITS ASSOCIATION WITH SOCIAL SUPPORT
Authors: Kong Lingming , Zhu Xiaoli , Niu Wei , Zhang Liyi 
Published: October 2021

To explore the differential expression of microRNA (miRNA, miR-) and its correlation with social support and personality in Generalized Anxiety Disorder (GAD) patients. A total of 80 GAD patients and 80 control participants were selected from our serial study samples to be tested for microarray analysis, and real time quantitative Polymerase Chain Reaction (qRT-PCR) verification was carried out in both patient group and control group. Correlation between miRNA expression and social support were also carried out. According to microarray analysis, 6 miRNAs were differentially expressed between patient group and control group, with miR-4505, miR-4484, miR-4674, miR-501-3p and miR-663 up-regulated, and miR-1301 down-regulated; qPCR verification revealed that 5 miRNAs (miR-1301, miR-4484, miR-4674, miR-501-3p, miR-663, miR-4505) were significantly up-regulated and the expression of miRNA-1301 down-regulated; the expression of miRNA-1301 was significantly related to social support in GAD patients. In conclusions, comprehensive factors, including miRNA regulation, social support may be involved into the aetiology of GAD.
Lines resistant to downy mildew in the sunflower genetic collection at VIR
Authors: Gavrilova V. A. , Stupnikova T. G. , Makarova L. G. , Alpatieva N. V. , Karabitsina Yu. I. , Kuznetsova E. B. , Anisimova I. A. 
Published: October 2021

Background. Downy mildew (DM) caused by the fungus Plasmopara halstedii (Farl) Berl. & De Toni) is one of the most harmful diseases of sunflower (Helianthus annuus L.). Due to the pathogen’s attacks, annual seed harvest losses range between 30% and 70%. Lines resistant to new races of the pathogen should be obtained for the development of commercial sunflower hybrids.Materials and methods. Downy mildew resistance of 323 lines and 10 cultivars from the sunflower collection was assessed in the field at the Kuban Experiment Station of VIR in 2017–2018. Line VIR 845, susceptible during all years of observations, was used as a control. Resistance genes were identified by means of the molecular analysis using diagnostic markers of the Plarg, Pl6 and Pl8 genes that confer resistance to many known P. halstedii races.Results. The founder varieties of VIR’s lines were susceptible to a varying extent. Thirty-nine lines were resistant in 2016 and 2018; among those, 36 lines were susceptible in 2017. Presumably, a more virulent P. halstedii race became widespread in 2017, compared to the races that prevailed in 2016 and 2018, so the genes that determined resistance in 2016 and 2018 turned out to be ineffective. Lines ТА 716-18, VIR 768, and VIR 800, having originated from interspecific hybrids, exhibited absence of pathogenic damage during 3 years of the trials. Molecular markers of the Plarg, Pl6 and Pl8 genes were detected in most lines that demonstrated resistance in 2016 and 2018. There were no markers in lines VIR 768 and VIR 800, whereas in ТА 716-18 the markers of Plarg and Pl8 were present.Conclusion. As a result of the long-term studies, a trait-specific genetic collection was established for sunflower. It comprises genotyped lines with various effective DM resistance genes. Lines ТА 716-18, VIR 768 and VIR 800 appeared highly resistant to the pathogen and probably possess new resistance genes/alleles introgressed from wild species.
Genetic diversity of wild stone fruit species: specific aspects of ex situ conservation
Authors: Eremin G. V. , Eremin V. G. , Chepinoga I. S. , Gasanova T. A. 
Published: October 2021

Background. One of the most important tasks faced by the scientists of Krymsk Experiment Breeding Station of VIR is the long-term preservation of the stone fruit gene pools (over 1,000 genotypes of 44 species) collected during plant explorations. It is much more difficult to preserve representatives of the wild flora than cultivars, due to their ecological specialization to specific environments and the need to create conditions for their successful development. This aspect concerns, first of all, such species as Louiseania pedunculata (Pall.) Pachom., L. ulmifolia (Franch.) Pachom., Prunus cocomilia Ten., P. brigantiaca Vill., Armeniaca sibirica (L.) Lam., Padus ssiori (F. Schmidt) C.K. Schneid., and some others.Methods. The studies were carried out in the collection plantations of the field genebank at Krymsk Experiment Breeding Station of VIR according to VIR’s guidelines. Accessions of wild stone fruit species were the objects of the studies.Results. Many years of field research into ex situ conservation of introduced wild species proved the efficiency of stone fruit cultivation in tub culture and a modified technique of dense planting with crown formation according to the “border-hedge” pattern. It takes into account biological characteristics of genotypes shaped in natural areas, the use of own-root or grafted plants on clonal rootstocks of various growth rates depending on their life form (tree or shrub), resistance to biotic and abiotic stressors, soil and terrain requirements, and the tasks posed before researchers. The recommended planting schemes (2.5–4.0 × 0.5–1.0 m) significantly reduce the area occupied by the same number of plants in conventional cultivation patterns and cut down power and labor inputs into technological practices.
Microsatellite loci variability in apple cultivars developed at VIR
Authors: Shlyavas A. V. , Trifonova A. A. , Chepinoga I. S. , Sidnin A. S. , Boris K. V. 
Published: October 2021

Background. Microsatellite (SSR) markers are now widely used both for studying genetic diversity in fruit crop collections, including apple (Malus × domestica Borkh.), and for genotyping individual accessions. The apple collection held by VIR, being the largest in Russia, contains cultivars bred at five experiment stations of VIR. These cultivars not only meet the requirements of horticulture in the region of their origin, but also are interesting as sources of valuable traits for breeding programs. However, these cultivars have not previously been studied using microsatellite markers. Materials and methods. A set of 10 SSR markers (CH02c02b, CH03d01, CH01f03b, CH02d08, CH02c02a, CH05e03, CH02c09, CHVf1, CH01h01, COL) was used for genotyping 35 apple cultivars developed at VIR and four parental forms. Fluorescently labeled PCR products were separated by capillary electrophoresis on ABI Prism 3130xl.Results. In 35 apple cultivars from VIR, 97 alleles were identified at ten studied loci. The number of alleles per locus varied from 5 (CH02c02b) to 17 (CH02c02a). The frequency of unique alleles was 26,8%, with 12,4% for rare alleles. Polymorphism information content (PIC) values varied from 0,49 (CH02c02b) to 0,91 (CH02c02a) and averaged 0,76. Expected and observed heterozygosity levels averaged 0,79 and 0,56, respectively. Cluster analysis did not reveal a clear division of cultivars according to the place of origin (experiment stations of VIR), but revealed clustering according to pedigrees.Conclusion. A high level of polymorphism was observed in 35 apple cultivars from VIR using ten SSR markers. The obtained data on the allelic composition of the studied cultivars can be used for their identification and contribute to more efficient collection management. Further studying and SSR genotyping of VIR’s apple collection and comparison with the data obtained in the presented work can help to identify the origin of cultivars with unknown pedigrees.
Anthecological features of Lonicera japonica Thunb. in the environments of Southern Uzbekistan
Authors: Djumayev Kh. K. , Tkachenko K. G. 
Published: October 2021

Background. Identification of spectacular ornamental perennial lianas for urban landscaping in southern cities is important for modern urban greening programs. The aim of this work was to study flowering peculiarities of Japanese honeysuckle (Lonicera japonica Thunb.).Materials and methods. The target material was honeysuckle plants growing in the environments of Southern Uzbekistan. Observations were conducted in 2019–2020 using conventional approaches.Results and conclusion. The inflorescence of L. japonica is an open dibotryoid, with flowers arranged along the lateral axes of the first order. Flowers are bisexual, zygomorphic. Blossoming of flowers in inflorescences occurs acropetally. The flowering period is almost 150–170 days (starts in April and lasts until the end of August). Dichogamy in the form of proterandry is observed in honeysuckle flowers. The male flowering phase comes the first. It starts 1.5–2.0 hours after the opening of the corolla and ends with the drying of the stamens. The corolla turns yellow 36 hours after the blooming of the flower and the stamens begin to dry out. This is the female phase. Over the next 84 hours, the pistil begins to dry out slowly, but the corolla of the flower lasts up to 96 hours. The duration of the female flowering phase is 60 hours. L. japonica is of considerable interest for vertical landscaping of urban communities. Plants remain ornamental for a long time. Studying this species as an essential oil plant is promising, since it contains a significant amount of germacrene D.
Mobilization of fruit, small fruit and nut crop genetic resources in Belarus
Authors: Kazlouskaya Z. A. , Fralova L. V. , Taranau A. A. , Yakimovich V. A. , Palubiatka I. G. 
Published: October 2021

Background. Natural populations of fruit and small fruit plants in Belarus and their specific and varietal composition for a long time attracted the attention of pomologists and breeders. Identification and mobilization of unique local forms and landraces as genetic sources of useful traits as well as monitoring duplicate collections will contribute to their more effective use in breeding practice to develop competitive domestic cultivars of various crops and reveal the adaptability of foreign cultivars to the conditions of Belarus.Methods. The itinerary of the collecting mission passed through 6 regions of the Republic of Belarus (Gomel, Minsk, Mogilev, Vitebsk, Brest, and Grodno) in 2012–2017.Results. As a result, 221 samples of fruit, small fruit and nut plants were collected, including 18 rootstocks of fruit crops. By 2021, 105 unique landraces were tested in the plant introduction and quarantine nursery and planted in the field gene bank, including 28 accessions of apple, 33 of pear, 11 of sour cherry, 5 of sweet cherry, 9 of plum, 8 of apricot, 1 of almond, 2 of hazel, 3 of garden strawberry, 1 of black chokeberry, 1 of viburnum, and 1 of bird cherry. There are plans to use the obtained accessions in breeding practice assources of valuable agronomic traits (high winter hardiness, high yield, resistance to a set of plant pathogens, etc.).
The impact of weather conditions in different years on the biochemical composition of linseed oil
Authors: Popova G. A. , Rogalskaya N. B. , Knyazeva N. V. , Trofimova V. M. , Shelenga T. V. , Porokhovinova E. A. , Brutch N. B. 
Published: October 2021

Background. Linseed oil is a versatile product with varying biochemical composition. Linseed breeding is aimed at producing cultivars with different chemical properties in their oil. The crop is widespread but the environment has a great effect on its fatty acid biosynthesis.Materials and methods. The impact of weather conditions on the variations in fatty acids composition was studied. Twenty linseed cultivars and accessions of diverse origin and with varying oil composition from the VIR collection were grown in Tomsk Province in 2016–2018. The content of 15 fatty acids (lauric, myristic, palmitic, palmitoleic, margaric, stearic, oleic, cis-vaccenic, linoleic, linolenic, arachidic, eicosenic, arachido nic, behenic, and lignoceric) was assessed in linseed oil using gas–liquid chromatography.Results. The tested material presented a wide diversity of genes controlling different steps of fatty acids biosynthesis and genetic mechanisms involved in the responses to the changing environment. The analysis of variance proved that the content of oleic, linoleic, linolenic and lignoceric acids was controlled solely by the genotype. The content of palmitic and stearic acids was influenced by both the genotype and environment. The synthesis of lauric and cis-vaccenic acids was significantly affected by the weather. Practically all acids, except linoleic and linolenic ones, showed a very high random variation, which in our experiment included genotype × environment interaction. The accessions differed in the range of variation in their characters under diffe rent conditions. There was no definite regularity in the hanges of fatty acid content in the tested genotypes during 3 years.Conclusion. The assessed genotypes probably possess different regulatory mechanisms for fatty acid biosynthesis. Thus, they present a diverse stock for further investigations into fatty acid biosynthesis and for the development of new linseed cultivars widely adaptable to environmental conditions.
THE ACS SELECTION MODEL FOR ROAD MILLING MACHINE
Authors: Ilge Igor 
Published: October 2021

The paper analyzes the problem of choosing an automatic control system for a road milling cutter for cold milling. The analysis of the ACS of the leading manufacturers was carried out, the typical structure of the ACS, the features of their functioning was determined, the necessity of building a selection model was substantiated, which would take into account the entire set of the main characteristics of the ACS of the road milling machine and make a scientifically grounded choice in conditions of uncertainty.
The purpose of this work is to increase the efficiency of the use of cold milling cutters by developing a model for choosing an ACS for a road milling cutter under uncertainty. To achieve this goal, the most appropriate is the use of the Analytic Hierarchy Process (methodology) based on the decomposition of the choice problem using a hierarchical structural model. Economic criteria (cost of ACS, operating costs), technical criteria (accuracy, speed, adaptive speed control, engine control, alignment of the cutter, working out of small radii), operational criteria (reliability, diagnostics and registration, ease of installation and adjustment, external control, service) and ergonomic criteria (visualization of parameters, operator comfort) that characterize the ACS of a road milling machine. The originality consists in the construction, on the basis of these groups of criteria, of a hierarchical structural model for choosing an ACS for a road milling cutter, which makes it possible to implement an Analytic Hierarchy Process and choose a reasonable alternative. The practicalvalue lies in the fact that the use of the developed model will make it possible to carry out a scientifically grounded choice of the ACS of the road milling cutter, taking into account the entire set of economic, technical, operational and ergonomic groups of criteria. In the course of further research, the software implementation of the developed model will be carried out in the form of an expert system for choosing the ACS of a road milling machine.