bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant Activities of Artemisia Absinthium and Citrus Paradisi Extracts Repress Viability of Aggressive Liver Cancer Cell Line
Authors: Ali Muhammad , Iqbal Rida , Safdar Muhammad , Murtaza Sehrish , Mustafa Ghulam , Sajjad Muhammad , Bukhari Shazia Anwer , Huma Tayyaba 
Published: July 2021

Abstract
Numerous reports show that herbal medicines can be utilized in the treatment of different liver disorders. In this study, antioxidant, antibacterial, and anticancer activities of individual as well as combined ethanolic extracts of Artemisia absinthium leaves and Citrus paradisi peels were investigated. Values of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH-radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured to explore the antioxidant capacity. To assess antibacterial activity, four bacterial strains were used. For evaluation of anticancer activity Huh-7 (liver cancer) and Vero (non-cancerous) cell lines were utilized. FRAP activity of combined plants extract was higher as compared to their individual effect; the trend did not hold in case of DPPH-radical scavenging activity. Disk diffusion method revealed that only combined extract of plants express antibacterial activity against one strain (E. coli). MTT results indicated that both plants have cytotoxic effect on Huh-7 cell line and did not show any effect on Vero cell line. Our data showed a strong negative correlation between the amount of TPC, TFC, & DPPH radicals-scavenging activity and cell viability of cancerous cell line. Whereas, no effect was shown on non-cancerous cell line. Hence, these plants can be used in treatment of liver cancer because of their antioxidant, antibacterial, and anticancer activities.
Antidepressant Like Effect of Sodium Orthovanadate in A Mouse Model of Chronic Unpredictable Mild Stress.
Authors: Joshi Angel , Akhtar Ansab , Saroj Priyanka , Kuhad Anurag , Sah Sangeeta Pilkhwal 
Published: July 2021

Abstract
Depression is a psychiatric disorder characterized by low esteem, anhedonia, social deficit, and lack of interest. Decreased BDNF and impaired TrKB signaling be associated with depression. In our study, depressive-like behavior was induced in mice by chronic unpredictable mild stress (CUMS) model. Various behavioral tests like tail suspension test (TST), open field test (OFT), and sucrose preference test (SPT); biochemical analyses for corticosterone, reduced glutathione (GSH), lipid peroxidation (LPO), superoxide dismutase (SOD), nitric oxide (NO) and ELISA for BDNF were performed. Body weight was measured every week. Depressive-like behavior was associated with increased oxidative stress in the brain and subsequent reduction of BDNF. Further, sodium orthovanadate (SOV), a protein tyrosine phosphatase inhibitor was used as a test drug as it is reported to stimulate BDNF levels. Sodium orthovanadate (SOV-5 mg/kg, 10 mg/kg) and fluoxetine (10 mg/kg) was given to mice orally for 21 days before 30 minutes of stress induction. The behavioral tests reflected depressive-like behavior in CUMS, which was attenuated by both SOV and fluoxetine. SOV at 10 mg/kg has demonstrated significant results in our study by decreasing malondialdehyde levels (MDA/LPO), NO levels, and increasing GSH and SOD in both the cortex and hippocampus. Besides, ELISA revealed the elevation of BDNF levels in the treatment groups (SOV-5 mg/kg, 10 mg/kg, and FLX-10 mg/kg) as compared with the disease group (CUMS). Therefore, the treatment with SOV appeared to reverse both oxidative and nitrosative stress. Decreased serum corticosterone levels (SOV-5 mg/kg, 10 mg/kg); FLX (10 mg/kg) + SOV (5 mg/kg); FLX-10 mg/kg and per-se) and elevated BDNF level (SOV-5 mg/kg, 10 mg/kg and FLX-10 mg/kg) were associated with attenuation of depressive-like behavior. The findings of this preliminary study indicate that SOV has the potential to restore antidepressant-like effect or prevention of stress-induced anhedonia and so further molecular mechanisms will be warranted for clinical translation.
‘Triple Clear’: A Systematic and Up-to-Date Surgical Process for Giant Cell Tumor of Bone Graded Companacci II and III with or without Pathological Fracture and Joint Invasion
Authors: Wang Yushan , Tian Qiaoqiao , Li Haoze , Li Jian , Ren Peng , Feng Yi 
Published: July 2021

Abstract
Objectives: 1) To describe a systematic process for giant cell tumours of bone (GCTB). 2) To compare the clinical effects of ‘triple clear’ (TC) and segmental resection (SR), bone grafts and bone cement.Method: Patients with primary GCTB graded Companacci II and III who were treated with either SR (n = 39) or TC (n = 41) were included. The pain level was determined by the Visual Analogue Scale. Limb function was determined by the Musculoskeletal Tumour Society (MSTS) score.Result: The operating time was 135.7±38.4 min in the TC group and 174.2±43.0 min in the SR group (P<0.05). The recurrence rates were 7.3% and 10.0%, respectively (P = 0.37). The MSTS scores at three months after surgery were 19.8±1.5 in the TC group and 18.8±1.5 in the SR group. The MSTS scores at two years were 26.3±0.7 and 24.2±1.6, respectively (P＜0.05).Conclusion: TC is recommended for GCTB graded Companacci II-III and GCTB accompanied by pathological fracture or joint invasion. Bone grafts may be more suitable than bone cement in the long term.
Intramyocardial Injection of Human Adipose-Derived Stem Cells Ameliorates Cognitive Deficit By Regulating Oxidative Stress-Mediated Hippocampal Damage After Myocardial Infarction
Authors: Lee Tsung-Ming , LEE Cheng-Che , HARN Horng-Jyh , CHIOU Tzyy-Wen , CHUANG Ming-Hsi , CHEN Chun-Hung , CHUANG Chi-Hsuan , LIN Po-Cheng , LIN Shinn-Zong 
Published: July 2021

Abstract
Cognitive impairment is a serious side effect of post-myocardial infarction (MI) course. We have recently demonstrated that human adipose-derived stem cells (hADSCs) ameliorated myocardial injury after MI by attenuating reactive oxygen species (ROS) levels. Here, we studied whether the beneficial effects of intramyocardial hADSC transplantation can extend to the brain and how they may attenuate cognitive dysfunction via modulating ROS after MI. After coronary ligation, male Wistar rats were randomized via an intramyocardial route to receive either vehicle, hADSC transplantation (1x106 cells), or the combination of hADSCs and 3-Morpholinosydnonimine (SIN-1, a peroxynitrite donor). Whether hADSCs migrated into the hippocampus was assessed by using human-specific primers in qPCR reactions. Passive avoidance test was used to assess cognitive performance. Postinfarction was associated with increased oxidative stress in myocardium, circulation and hippocampus. This was coupled with decreased numbers of dendritic spines as well as a significant downregulation of synaptic plasticity consisting of synaptophysin and PSD95. Step through latency during passive avoidance test was impaired in vehicle-treated rats after MI. Intramyocardial hADSC injection exerted therapeutic benefits in improving cardiac function and cognitive impairment. None of hADSCs were detected in rat’s hippocampus at the 3th day after intramyocardial injection. The beneficial effects of hADSCs on MI-induced histological and cognitive changes were abolished after adding SIN-1. MI-induced ROS attacked the hippocampus to induce neurodegeneration, resulting in cognitive deficit. The remotely intramyocardial administration of hADSCs, has the capacity of improved synaptic neuroplasticity in hippocampus mediated by ROS, not the cell engraftment, after MI.
Bleaching Flax Roving With Poly(Acrylic Acid) Magnesium Salt As Oxygen Bleaching Stabilizer For Hydrogen Peroxide
Authors: Liu Jie , Jiang Wenqi , Sun Ling , Lv Chun 
Published: July 2021

Abstract
Alkali-oxygen one-bath scouring and bleaching process of the flax roving was studied by using a new type of synthesized non-silicon oxygen bleaching stabilizer Poly(acrylic acid) magnesium instead of sodium silicate. Based on the analysis of the effects of single factors such as sodium hydroxide concentration, hydrogen peroxide concentration, temperature, time and the amount of the synthesized non-silicon oxygen bleaching stabilizer poly(acrylic acid) magnesium salt on the performance of the bleached flax roving, including the whiteness, the breaking tenacity, the capillary effect and the weight loss ratio. The optimal process for the application of the stabilizer was determined by orthogonal test, namely, hydrogen peroxide concentration 8.5 g/L, sodium hydroxide concentration 5 g/L, sodium bisulfite 3 g/L, sodium carbonate 3 g/L, the synthesized non-silicon oxygen bleaching stabilizer poly(acrylic acid) magnesium 5.5 g/L, scoured and bleached at 90 ℃ for 60 min, and the bath ratio was 25:1. Compared with the traditional oxygen bleaching stabilizer sodium silicate, it not only has good ability to inhibit the rapid decomposition of hydrogen peroxide, but also has the advantages of higher whiteness, higher capillary effect, good feel and breaking tenacity, and can effectively solve the "silicon scale" problem and improve the quality of flax products.
Study of Cadmium Desorption Kinetics from Riverine Sediments
Authors: Nasrabadi Mohsen , Omid Mohammad Hossein , Mazdeh Ali Mahdavi 
Published: July 2021

Abstract
In natural streams, the majority of heavy metal ions are generally associated ‎with ‎sediment particles. Under some environmental conditions, these metal ions may release from ‎the ‎sediment particles.‎ In such conditions, the desorption rate of heavy metals is very important for decision-makers of ‎water quality assessment. In this study, the effect of cadmium desorption ‎from the river bed ‎sediments has been ‎experimentally investigated. Artificially contaminated sediments were used ‎for performing batch desorption ‎experiments. The experiments were conducted by adding 1 gr of contaminated ‎sediment (D50 = 0.53 mm) with a known concentration and shaking until observing a ‎roughly ‎constant cadmium concentration in the solution. It was concluded that the cadmium ions were strongly bond to the river bed sediment; meanwhile, at the ‎equilibrium time, up to about 7 to 29 percent of cadmium ions were ‎released from the‎ artificially contaminated sediments. The experiments were followed by ‎two agitation rates of 100 and 200 rpm. It was revealed that by increasing the flow turbulence, the amount ‎of desorbed cadmium is slightly increased. Besides, the desorption kinetics was evaluated using eight models of Zero-, first-, second-, third-order, ‎parabolic diffusion, double parabolic diffusion, two constant rate, and simple Elovich. The results of the evaluation showed that simple Elovich (with R2 = 0.991), double parabolic diffusion (with R2 = 0.9882), two constant rate (with R2 = 0.983) and parabolic diffusion models (with R2 = 0.846) have respectively the best performance in calculation of Cd desorption rate from the sediments.
Socio-Territorial Inequities in the French National Breast Cancer Screening Programme – A Cross-Sectional Multilevel Study
Authors: Rollet Quentin , Guillaume Élodie , Launay Ludivine , Launoy Guy 
Published: July 2021

Abstract

Background. France implemented in 2004 the French National Breast Cancer Screening Programme (FNBCSP). Despite national recommendations, this programme coexists with non-negligible opportunistic screening practices.Aim. Analyse socio-territorial inequities in the 2013-2014 FNBCSP campaign in a large sample of the eligible population.Method. Analyses were performed using three-level hierarchical generalized linear model. Level one was a 10% random sample of the eligible population in each département (n = 397 598). For each woman, age and travel time to the nearest accredited radiology centre were computed. These observations were nested within 22 250 IRIS, for which the European Deprivation Index (EDI) is defined. IRIS were nested within 41 départements, for which opportunistic screening rates and gross domestic product based on purchasing power parity were available, deprivation and the number of radiology centres for 100 000 eligible women were computed. Results. Organized screening uptake increased with age (OR1SD = 1.05 [1.04 – 1.06]) and decreased with travel time (OR1SD = 0.94 [0.93 – 0.95]) and EDI (OR1SD = 0.84 [0.83 – 0.85]). Between départements, organized screening uptake decreased with opportunistic screening rate (OR1SD = 0.84 [0.79 – 0.87]) and départements deprivation (OR1SD = 0.91 [0.88 – 0.96]). Association between EDI and organized screening uptake was weaker as opportunistic screening rates and as département deprivation increased. Heterogeneity in FNBCSP participation decreased between IRIS by 36% and between départements by 82%. Conclusion. FNBCSP does not erase socio-territorial inequities. The population the most at risk of dying from BC is thus the less participating. More efforts are needed to improve equity.
Impact of Soil Moisture on Crop Yields in Major Rainfed Growing Regions of Peninsular India
Authors: Bal Santanu Kumar , Shivaramu H. S. , Kumar P. Vijaya , Lingaraj H. , Sandeep V. M. , Rao A. V.M. Subba , Singh V. K. , Manjunatha M. H. , Malleswari S. N. , Tupe Arvind , Sudhakar G. , Dakhore K. K. , Subbulakshmi S. 
Published: July 2021

Abstract
Assessment of soil moisture availability and timely declaration of drought are keys for exemplary relief assistance in water stressed regions. Percent available soil moisture (PASM) is one among several drought declaration indices, needs evaluation with respect to individual crop and cropping system, as the amount of water requirement varies with respect to crop and its growth stage. Analysis of yield variability due to PASM was carried out by employing correlation and linear regression analyses based on long term observations in experiments conducted at different dry farming locations of the peninsular India. The range of available soil moisture in order to obtain at least 50 per cent of optimum yield in cereals (maize: 26 and finger millet: 52.9 PASM), pulses (pigeonpea: 37.2 PASM), oilseeds (soybean: 26.8 to 30.5, groundnut: 53.8 to 61.7 PASM) and commercial crops (cotton: 26.3 PASM) was 26 to 61 per cent. Establishment of these regression models helped in timely drought declaration / precise identification of drought hit areas and assuring feasible relief assistance. The outcomes of the study may be used for amending the existing drought norms (0–50; severe, 50–75; mild and 75–100; no drought) for provision of proportionate compensations to the farmers.
Magnitude of Anxiety and Associated Factors Among Epileptic Patients Attending Out-patient Department at Primary Public Hospitals in Northern Part of Ethiopia. A Multicenter Cross- Sectional Study
Authors: Nigussie Kabtamu , Sertsu Addisu 
Published: July 2021

Abstract
Introduction: In spite of the fact that anxiety disorders are common among individuals with epilepsy, there are no studies conducted about the prevalence of anxiety disorders among individuals with epilepsy in Ethiopia. Hence, the point of this study was to determine the magnitude and related factors of anxiety among individuals with epilepsy attending an out-patient department at Central Gondar Zone Primary Public Hospitals, Northern Ethiopia.Method: An organizational based cross-sectional study was conducted from June - July, 2020 at central Gondar zone primary public hospitals. A total of 422 participants were chosen by systematic sampling technique. Information was collected by utilizing Amharic interviewer-administered questioners. Anxiety was assessed by using hospital depression anxiety scale. Bivariate logistic regression and multivariate logistic regression analyses were done to recognize variables related with anxiety. Association was described by using adjusted odds ratio (AOR) along with 95% Confidence interval. Finally, P-values < 0.05 in adjusted analysis were taken as a cut off for significant association. Result: Out of 412 participants included in the study, 116 (28.2%; 95% CI: 23.9-32.5) had anxiety. Being single (AOR=1.84, 95% CI: 1.04-3.24), very frequent frequency of seizure (AOR=1.24, 95% CI; 1.55-2.80), depression (AOR=3.62, 95% CI; 1.97- 8.66), perceived stigma (AOR=2.54, 95% CI; 1.83-4.67) and hazardous alcohol use (AOR=1.18, 95% CI, 1.53-3.63), were statically significantly associated with having anxiety. Conclusion: Nearly, one in four people with epilepsy visited out-patient treatment had anxiety. Being single, very frequent frequency of seizure, having depression, presence perceived stigma and hazardous alcohol use were significant associated with anxiety among individuals with epilepsy. Screening, early distinguishing proof and providing appropriate intervention of anxiety among individuals with epilepsy should be great concern for the wellbeing care suppliers.
Prediction of Pasture Intake by Beef Cattle in Tropical Conditions
Authors: Fernandes Geferson Antonio , de Oliveira André Soares , de Araújo Cláudio Vieira , Couto Victor Rezende Moreira , de Moraes Kamila Andreatta Kling , de Moraes Eduardo Henrique Bevitori Kling 
Published: July 2021

Abstract
A meta-analytic study was conducted to develop and evaluate models for the prediction of pasture dry matter intake (DMIpasture) by beef cattle in tropical conditions. 804 individual observations of DMIpasture were used, taken from 23 studies through analysis of mixed models, including the study as a random effect. To evaluate the accuracy and precision of the new models proposed as well as for the models of Azevedo et al., (2016) and Minson and McDonald (1987), an independent databank with 87 means from treatments of 21 experiments (n = 888 animals) was used. Three prediction models were adjusted: Model I (Animal Information), Model II (Animal Information + Supplement) and Model III (Animal Information + Supplement + Pasture). The proposed models presented similarity for the average square root of the prediction error. The inclusion of the predictive variables for supplementation (supplement dry matter intake - DMIsupplement - % of the body weight and crude protein intake through supplement) with the variables for the animal (BW0,75 and average daily gain) and of the pasture (% of crude protein) in Model III improves accuracy and precision and provides higher determination and correlation coefficients, and agreement than the other proposed models. Similarly, it was found to be more accurate and precise than the equations of Azevedo et al., (2016) and Minson and McDonald (1987), which presented lower precision and accuracy than the proposed models. The DMIpasture for beef cattle in tropical conditions is more accurate and precise when the information for the animal, supplement and pasture is included.
Molecular Characterization and Overexpression of CYP51 gene of Difenoconazole Resistance in Lasiodiplodia Theobromae Field Isolates
Authors: Wang Chenguang , Xu Luxi , Liang Xiaoyu , Wang Jing , Xian Xinwei , Zhang Yu , Yang Ye 
Published: July 2021

Abstract
Stem-end rot (SER) caused by Lasiodiplodia theobromae is an important disease of mango in China. Demethylation inhibitor (DMI) fungicide are widely used for diseases control in mango orchards. The baseline sensitivity to difenoconazole of 138 isolates collected in the field in 2019 from mango were established by the mycelial growth rate method. The cross-resistance to six site-specific fungicides with different modes of action were investigated using 20 isolates randomly selected. The possible reasons for L. theobromae resistance to difenoconazole were preliminarily determined through gene sequence alignment and quantitative real-time PCR analysis. The results showed that the EC50 values of 138 L. theobromae isolates to difenoconazole ranged from 0.01 to 13.72 µg/ml. The frequency of difenoconazole sensitivity formed a normal distribution curve when the outliers were excluded. Difenoconazole showed positive cross-resistance only with the DMI tebuconazole, but not with non-DMI carbendazim, pyraclostrobin, fludioxonil, bromothalonil, or iprodione. Some multifungicide-resistant isolates of L. theobromae were found. Two amino acid substitutions (E209k and G207A) were found in CYP51 protein, but they were not likely related to the resistance phenotype. There was no alteration in promoter region of the CYP51 gene. However, difenoconazole significantly increased the expression of the CYP51 gene in the resistant isolates when compared to the susceptible isolates. This study is important references to explore resistance mechanism. These results are vital to make effective mango diseases management strategies in order to avoid the development of further resistance.
Isolation and Screening for Ureolytic Bacteria from Bukilat Cave, Camotes Islands, Cebu
Authors: Nery Virjun Rae , Bureros Kenneth Joseph 
Published: July 2021

Abstract
Microbial-induced calcite precipitation (MICP) is an eco-friendly technique used in creating better soil substrate often for engineering purposes. This is done through the application of the ureolytic pathway of certain bacteria. This study aims to discover whether any of these bacteria can be found in Bukilat Cave, Camotes Islands, Cebu. Samples from the pools of water, drip water, and swabs of the walls of the cave were collected, cultured, and then tested using Christensen’s agar for their ability to undergo the ureolytic pathway. The rate at which they undergo the ureolytic pathway was then measured and compared between different sources and to the positive control, Bacillus megaterium. The results showed that there was no significant difference between the rate at which bacteria from the different sources underwent ureolysis. There was also no significant difference between the rate at which the collected bacteria underwent ureolysis and the rate of the positive control (2.588 mM/min). Finally, the species with the fastest rate of ureolysis was identified to be Bacillus cereus NR 074540 with a rate of 3.033 mM/min. However, it is not ideal for MICP purposes because of its potential pathogenicity.