bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flavoured warped axion
Authors: Bonnefoy Quentin , Cox Peter , Dudas Emilian , Gherghetta Tony , Nguyen Minh D. 
Published: April 2021

Abstract
We consider a 5D extension of the DFSZ axion model that addresses both the axion quality and fermion mass hierarchy problems, and predicts flavour-dependent, off-diagonal axion-fermion couplings. The axion is part of a 5D complex scalar field charged under a U(1)PQ symmetry that is spontaneously broken in the bulk, and is insensitive to explicit PQ breaking on the UV boundary. Bulk Standard Model fermions interact with two Higgs doublets that can be localized on the UV boundary or propagate in the bulk to explain the fermion masses and mixings. When the Higgs doublets are localized on the UV boundary, they induce flavour diagonal couplings between the fermions and the axion. However, when the Higgs doublets propagate in the bulk, the overlap of the axion and fermion profiles generates flavour off-diagonal couplings. The effective scale of these off-diagonal couplings in both the quark and lepton sectors can be as small as 1011 GeV, and therefore will be probed in future precision flavour experiments.
Circular Wilson loop in $$ \mathcal{N} $$ = 2* super Yang-Mills theory at two loops and localization
Authors: Belitsky A. V. , Korchemsky G. P. 
Published: April 2021

Abstract
We present a two-loop calculation of the supersymmetric circular Wilson loop in the $$ \mathcal{N} $$
N
= 2* super Yang-Mills theory on the four-sphere. We develop an efficient framework for computing contributing Feynman graphs that relies on using the embedding coordinates combined with the Mellin-Barnes techniques for propagator-like integrals on the sphere. Our results exactly match predictions of supersymmetric localization providing a nontrivial consistency check for the latter in non-conformal settings.
Deep learning assistance increases the detection sensitivity of radiologists for secondary intracranial aneurysms in subarachnoid hemorrhage
Authors: Pennig Lenhard , Hoyer Ulrike Cornelia Isabel , Krauskopf Alexandra , Shahzad Rahil , Jünger Stephanie T. , Thiele Frank , Laukamp Kai Roman , Grunz Jan-Peter , Perkuhn Michael , Schlamann Marc , Kabbasch Christoph , Borggrefe Jan , Goertz Lukas 
Published: April 2021

Abstract
Purpose
To evaluate whether a deep learning model (DLM) could increase the detection sensitivity of radiologists for intracranial aneurysms on CT angiography (CTA) in aneurysmal subarachnoid hemorrhage (aSAH).

Methods
Three different DLMs were trained on CTA datasets of 68 aSAH patients with 79 aneurysms with their outputs being combined applying ensemble learning (DLM-Ens). The DLM-Ens was evaluated on an independent test set of 104 aSAH patients with 126 aneuryms (mean volume 129.2 ± 185.4 mm3, 13.0% at the posterior circulation), which were determined by two radiologists and one neurosurgeon in consensus using CTA and digital subtraction angiography scans. CTA scans of the test set were then presented to three blinded radiologists (reader 1: 13, reader 2: 4, and reader 3: 3 years of experience in diagnostic neuroradiology), who assessed them individually for aneurysms. Detection sensitivities for aneurysms of the readers with and without the assistance of the DLM were compared.

Results
In the test set, the detection sensitivity of the DLM-Ens (85.7%) was comparable to the radiologists (reader 1: 91.2%, reader 2: 86.5%, and reader 3: 86.5%; Fleiss κ of 0.502). DLM-assistance significantly increased the detection sensitivity (reader 1: 97.6%, reader 2: 97.6%,and reader 3: 96.0%; overall P=.024; Fleiss κ of 0.878), especially for secondary aneurysms (88.2% of the additional aneurysms provided by the DLM).

Conclusion
Deep learning significantly improved the detection sensitivity of radiologists for aneurysms in aSAH, especially for secondary aneurysms. It therefore represents a valuable adjunct for physicians to establish an accurate diagnosis in order to optimize patient treatment.
Gut microbiota profiles and characterization of cultivable fungal isolates in IBS patients
Authors: Sciavilla Piero , Strati Francesco , Di Paola Monica , Modesto Monica , Vitali Francesco , Cavalieri Duccio , Prati Gian Maria , Di Vito Maura , Aragona Giovanni , De Filippo Carlotta , Mattarelli Paola 
Published: April 2021

AbstractStudies so far conducted on irritable bowel syndrome (IBS) have been focused mainly on the role of gut bacterial dysbiosis in modulating the intestinal permeability, inflammation, and motility, with consequences on the quality of life. Limited evidences showed a potential involvement of gut fungal communities. Here, the gut bacterial and fungal microbiota of a cohort of IBS patients have been characterized and compared with that of healthy subjects (HS). The IBS microbial community structure differed significantly compared to HS. In particular, we observed an enrichment of bacterial taxa involved in gut inflammation, such asEnterobacteriaceae,Streptococcus,Fusobacteria,Gemella, andRothia, as well as depletion of health-promoting bacterial genera, such asRoseburiaandFaecalibacterium. Gut microbial profiles in IBS patients differed also in accordance with constipation. Sequence analysis of the gut mycobiota showed enrichment ofSaccharomycetesin IBS. Culturomics analysis of fungal isolates from feces showed enrichment ofCandidaspp. displaying from IBS a clonal expansion and a distinct genotypic profiles and different phenotypical features when compared to HS ofCandida albicansisolates. Alongside the well-characterized gut bacterial dysbiosis in IBS, this study shed light on a yet poorly explored fungal component of the intestinal ecosystem, the gut mycobiota. Our results showed a differential fungal community in IBS compared to HS, suggesting potential for new insights on the involvement of the gut mycobiota in IBS.Key points•Comparison of gut microbiota and mycobiota between IBS and healthy subjects•Investigation of cultivable fungi in IBS and healthy subjects•Candida albicans isolates result more virulent in IBS subjects compared to healthy subjects
Genetic alterations in Thai adult patients with acute myeloid leukemia and myelodysplastic syndrome—excess blasts detected by next-generation sequencing technique
Authors: Owattanapanich Weerapat , Herzig Julia , Jahn Nikolaus , Panina Ekaterina , Ruchutrakool Theera , Kungwankiattichai Smith , Issaragrisil Surapol , Döhner Hartmut , Döhner Konstanze 
Published: April 2021

AbstractSeveral molecular aberrations affect the prognosis of patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) with excess blasts (EB). This study aimed to determine the incidence and clinical impact of molecular genetic aberrations in Thai patients with AML and MDS-EB, detected by the next-generation sequencing (NGS) technique. This prospective, observational study was conducted between 2018 and 2020 on newly diagnosed Thai AML or MDS-EB patients aged above 15 years. NGS was performed using a custom amplicon-based targeted enrichment assay for 42 genes recurrently mutated in myeloid neoplasms. The molecular results were correlated with baseline patient and disease characteristics as well as outcomes. Forty-nine patients were enrolled in this study. The median age was 56 years (interquartile range [IQR], 44–64), with nearly equal proportions of males and females. The median number of mutations was 3 (IQR, 2–4). The most frequent alterations were FLT3 internal tandem duplications (ITD) (28.6%), DNMT3A (24.5%), and WT1 (22.4%) mutations. FLT3-ITD was more frequent in the de novo AML group than in the MDS/secondary AML group, whereas in the MDS/secondary AML group, ASXL1, ETV6, and SRSF2 mutations were more frequent. Patients aged greater than 65 years and patients with mutated TP53 were more likely to have inferior overall survival from multivariate analysis. FLT3-ITD was the most common mutation among newly diagnosed Thai AML patients. TP53 mutation and advanced age were independent adverse factors for survival outcome. The genetic landscapes of AML patients vary between national populations. Thai Clinical Trials Registry identifier: TCTR20190227003.
Interannual variability in the subduction of the South Atlantic subtropical underwater
Authors: Liu Hao , Li Shujiang , Wei Zexun 
Published: April 2021

AbstractThe South Atlantic subtropical underwater (STUW) is a high-salinity water mass formed by subduction within the subtropical gyre. It is a major component of the subtropical cell and affects stratification in the downstream direction due to its high salinity characteristics. Understanding the interannual variability in STUW subduction is essential for quantifying the impact of subtropical variability on the tropical Atlantic. Using the output from the ocean state estimate of the Consortium for Estimating the Circulation and Climate of the Ocean (ECCO), this study investigates the interannual variability in STUW subduction from 1992 to 2016. We find that heat fluxes, wind stress, and wind stress curl cause interannual variability in the subduction rate. Heat fluxes over the subduction area modulate the sea surface buoyancy and regulate the mixed layer depth (MLD) during its deepening and shoaling phases. Additionally, the wind stress curl and zonal wind stress can modulate the size of the subduction area by regulating the probability of particles entrained into the mixed layer within 1 year of tracing. This analysis evaluates the influence of subtropical wind patterns on the South Atlantic subsurface high-salinity water mass, highlighting the impact of heat and wind on the interannual changes in the oceanic component of the hydrological cycle.
The histopathological synovitis score is influenced by biopsy location in patients with knee osteoarthritis
Authors: Mussawy Haider , Zustin Jozef , Luebke Andreas M. , Strahl André , Krenn Veit , Rüther Wolfgang , Rolvien Tim 
Published: April 2021

AbstractIntroductionOsteoarthritis (OA) and rheumatoid arthritis (RA) represent the most common forms of arthritis, which are mainly caused by mechanical and inflammatory components, respectively. Determination of synovial inflammation in synovial biopsies via the histopathological Krenn score may be crucial for correct diagnosis and treatment. Specifically, it remains unclear whether synovitis scores differ among multiple biopsy locations within a single joint.Materials and methodsEighty synovial samples were taken from four standardized regions of the knee in 20 patients (ten primary OA, ten secondary OA) undergoing total knee arthroplasty (TKA) or total synovectomy. The Krenn synovitis score (grade 0–9) was determined in a blinded manner by two expert pathologists in all biopsies. Next to the inter-rater reliability, we evaluated the agreement of the determined scores among the four biopsy locations within each knee.ResultsThe inter-rater reliability between the two pathologists was very high (Cohen’s kappa = 0.712;r = 0.946; ICC = 0.972). The mean synovitis score was significantly higher in knees with secondary than in primary OA (p = 0.026). Importantly, we found clear differences between the scores of the four different biopsy locations within the individual knee joints, with an average deviation of 10.6%. These deviations were comparable in knees with primary and secondary OA (p = 0.64).ConclusionsWhile we confirmed the synovitis score as a reliable and reproducible parameter to assess the histopathological synovitis grade in the knee, the considerable variability within the joint indicates that multiple synovial biopsies from different regions should be obtained to enable reliable results of the synovitis score.
A survey of vocal health in church choir singers
Authors: Sharma Vasudha , Nayak Srikanth , Devadas Usha 
Published: April 2021

Abstract
Purpose
Choir singing is an important tradition of Christian worship across India. However, vocal health issues related to the church choir singers are less addressed in the literature. Hence, this study aimed to investigate the prevalence of vocal symptoms, identify the variables associated with increased risk of voice problems and knowledge of factors influencing vocal health in church choir singers.

Method
One hundred and forty-eight church choir singers (61 males and 85 females) between the age range of 18 and 70 years participated in the study. They completed a self-reported questionnaire addressing demographic and singing-related details, vocal symptoms, variables associated with increased risk reporting voice problems and knowledge about factors influencing vocal health.

Result
Eighty-four percent of the choir singers reported two or more vocal symptoms sometimes or more frequently while or after singing. More than half of the church choir singers had experienced vocal symptoms such as accessing notes in the upper range, loss of vocal endurance, pitch breaks, hoarseness, dryness in the throat, and discomfort in the throat. Among the different variables, systemic hydration found to have a significant association with reporting of voice problems in church choir singers. The overall knowledge regarding the factors influencing vocal health was found to be limited among the choir singers.

Conclusion
Choir singers like other professional singers experienced a higher prevalence of vocal symptoms during or after singing and exhibited limited knowledge about factors that negatively influence vocal health. Hence, there is a need to look into these singer’s vocal requirements, who usually go unnoticed.
Methods for restoration of ki67 antigenicity in aged paraffin tissue blocks
Authors: Grillo Federica , Campora Michela , Pigozzi Simona , Bonadio Silvia , Valle Luca , Ferro Jacopo , Paudice Michele , Dose Beatrice , Mastracci Luca 
Published: April 2021

AbstractPathology archives are a treasure trove of paraffin embedded tissue spanning many years and covering a wide variety of tissues and diseases. The possibility of using old archival formalin fixed paraffin embedded (FFPE) tissues for diagnostic updates and research projects is a widespread need and it requires archives of stable, well-preserved samples. Immunohistochemistry performed on old archival paraffin blocks may give unreliable results, in particular for some antigens, such as Ki67. In consideration of this phenomenon, our aim is to comprehensively test and identify methods which may be used to obtain Ki67 immunohistochemical reactions of good quality from old archival FFPE blocks. Various methods were tested in order to evaluate their possible efficacy in increasing Ki67 immunointensity in a collection of 40-year-old, archival blocks including re-embedding, with deeper sectioning of tissue from the block and increasing heat-based pretreatment times (20 cases) and re-processing (20 cases). All reactions were performed using an automated immunostainer and Ki67 stained immunosections compared using a visual colour-based scale (the first immunostained section was considered as baseline). The combination of deep sectioning (1000 µM) and prolonged heat-based pretreatment (64 min) markedly increased immunoreactivity for Ki67. Re-embedding and reprocessing did not have a significant effect. Large tissue samples showed heterogeneity of Ki67 immunoexpression between the periphery of the sample and the central area. In conclusion, the study defines a useful protocol to increase antigen retrieval applicable to dated archival tissues.
Shallow crystallization of eruptive magma inferred from volcanic ash microtextures: a case study of the 2018 eruption of Shinmoedake volcano, Japan
Authors: Matsumoto Keiko , Geshi Nobuo 
Published: April 2021

AbstractThe occurrence of groundmass crystals reveals the shallow conduit process of magmas, which affects the behavior of eruptions. Here, we analyzed groundmass microtextures of ash samples from the 2018 eruption of Shinmoedake volcano, Japan, to evaluate the change of magma ascent conditions during the eruption sequence. The eruptive activity changed from ash venting (Phase 1: March 1–6) to lava effusion with continuous ash-laden plumes (Phase 2: March 6–9) and then shifted to Vulcanian explosions (Phase 3: March 10–April 5). Non-juvenile particles were abundant in Phase 1, whereas juvenile particles were dominant in Phases 2 and 3. Vesicular juvenile particles were more abundant in Phase 2 than Phase 3. The lower microlite crystallinity and groundmass SiO2 concentrations of the vesicular particles indicate that they were sourced from magma that ascended rapidly. Abundant nanolites were observed in the black interstitial glass of juvenile particles under an optical microscope, whereas few nanolites were observed in the transparent ones. The presence of nanolites can be explained by the dehydration of silicate melt, as well as cooling and oxidation between fragmentation and quenching. Temporal changes in the ash componentry show that the eruption activity started from the erosion of the pre-existing vent plug (Phase 1), shifted to the simultaneous eruption of bubble-bearing and outgassed magmas (Phase 2), and concluded with explosions of the stagnant lava (Phase 3), thereby demonstrating the sequence of vent opening and extrusion and stagnation of magma. Therefore, ash microtextures are valuable for monitoring the shallow conduit process of eruptive magma.
Immunohistochemical analyses on two distinct internodes of stinging nettle show different distribution of polysaccharides and proteins in the cell walls of bast fibers
Authors: Faleri Claudia , Xu Xuan , Mareri Lavinia , Hausman Jean-Francois , Cai Giampiero , Guerriero Gea 
Published: April 2021

AbstractStinging nettle is a perennial herbaceous species holding value as a multi-purpose plant. Indeed, its leaves and roots are phytofactories providing functional ingredients of medicinal interest and its stems produce silky and resistant extraxylary fibers (a.k.a. bast fibers) valued in the biocomposite sector. Similarly to what is reported in other fiber crops, the stem of nettle contains both lignified and hypolignified fibers in the core and cortex, respectively, and it is therefore a useful model for cell wall research. Indeed, data on nettle stem tissues can be compared to those obtained in other models, such as hemp and flax, to support hypotheses on the differentiation and development of bast fibers. The suitability of the nettle stem as model for cell wall-related research was already validated using a transcriptomics and biochemical approach focused on internodes at different developmental stages sampled at the top, middle, and bottom of the stem. We here sought to complement and enrich these data by providing immunohistochemical and ultrastructural details on young and older stem internodes. Antibodies recognizing non-cellulosic polysaccharides (galactans, arabinans, rhamnogalacturonans) and arabinogalactan proteins were here investigated with the goal of understanding whether their distribution changes in the stem tissues in relation to the bast fiber and vascular tissue development. The results obtained indicate that the occurrence and distribution of cell wall polysaccharides and proteins differ between young and older internodes and that these changes are particularly evident in the bast fibers.
Biologically effective dose correlates with linear tumor volume changes after upfront single-fraction stereotactic radiosurgery for vestibular schwannomas
Authors: Tuleasca Constantin , Faouzi Mohamed , Maeder Philippe , Maire Raphael , Knisely Jonathan , Levivier Marc 
Published: April 2021

AbstractVestibular schwannomas (VSs) are benign, slow-growing tumors. Management options include observation, surgery, and radiation. In this retrospective trial, we aimed at evaluating whether biologically effective dose (BED) plays a role in tumor volume changes after single-fraction first intention stereotactic radiosurgery (SRS) for VS. We compiled a single-institution experience (n = 159, Lausanne University Hospital, Switzerland). The indication for SRS was decided after multidisciplinary discussion. Only cases with minimum 3 years follow-up were included. The Koos grading, a reliable method for tumor classification was used. Radiosurgery was performed using Gamma Knife (GK) and a uniform marginal prescription dose of 12 Gy. Mean BED was 66.3 Gy (standard deviation 3.8, range 54.1–73.9). The mean follow-up period was 5.1 years (standard deviation 1.7, range 3–9.2). The primary outcome was changes in 3D volumes after SRS as function of BED and of integral dose received by the VS. Random-effect linear regression model showed that tumor volume significantly and linearly decreased over time with higher BED (p < 0.0001). Changes in tumor volume were also significantly associated with age, sex, number of isocenters, gradient index, and Koos grade. However, the effect of BED on tumor volume change was moderated by time after SRS and Koos grade. Lower integral doses received by the VSs were inversely correlated with BED in relationship with tumor volume changes (p < 0.0001). Six (3.4%) patients needed further intervention. For patients having uniformly received the same marginal dose prescription, higher BED linearly and significantly correlated with tumor volume changes after SRS for VSs. BED could represent a potential new treatment paradigm for patients with benign tumors, such as VSs, for attaining a desired radiobiological effect. This could further increase the efficacy and decrease the toxicity of SRS not only in benign tumors but also in other SRS indications.
Economic evidence with respect to cost-effectiveness of the transitional care model among geriatric patients discharged from hospital to home: a systematic review
Authors: Kast Kristina , Wachter Carl-Philipp , Schöffski Oliver , Rimmele Martina 
Published: April 2021

AbstractBackgroundThe German hospital-to-home discharge management of geriatric patients has long been criticized. The implementation of the American Transitional Care Model (TCM) could help to reduce readmissions and costs. The objective of this review was to check the scientific evidence of the cost-effectiveness of the TCM.MethodsA systematic literature search in six databases for the time period of 26 years was conducted. The studies had to meet all pre-defined inclusion criteria. The data extraction is based on a criteria chart from literature. The methodological quality was assessed using the tools of the National Heart, Lung, and Blood Institute as well as the Consensus Health Economic Criteria list. The results transferability to German health care system was explained based on the criteria from the literature.ResultsThree American studies met all criteria. They showed partial cost analyses but no full economic analyses. It could be assumed that the economic effect of the TCM changes over time. The costs of a care coordinator could not be determined because few detailed information was reported. The TCM may have negative consequences for hospitals. The results are not transferable to Germany.ConclusionThere is no scientific evidence for the cost-effectiveness of the defined TCM. The optimal TCM duration still needs to be clarified. A detailed overview with units and prices and an additional consideration of the hospital perspective could help to make the information more transparent when deciding about the TCM implementation. A full economic analysis under German conditions or for similar European countries is necessary.