bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liver transplant after donation from controlled circulatory death versus brain death: A UNOS database analysis and publication bias adjusted meta‐analysis
Authors: Ziogas Ioannis A. , Kakos Christos D. , Esagian Stepan M. , Skarentzos Konstantinos , Alexopoulos Sophoclis P. , Shingina Alexandra , Montenovo Martin I. 
Published: October 2021

AbstractBackgroundDonation after circulatory death (DCD) is an increasingly utilized alternative to donation after brain death (DBD) to expand the liver donor pool. We compared the outcomes of liver transplant (LT) after DCD versus DBD.MethodsA PRISMA‐compliant systematic literature review was performed using the PubMed, Cochrane Library, and Embase databases (end‐of‐search‐date: July 2, 2020). US outcomes were analyzed using the UNOS database (February, 2002–September, 2020). Pooled hazard ratios (HR) for patient and graft survival were obtained through random effect meta‐analyses and adjusted for publication bias.ResultsThirteen studies reporting on 1426 DCD and 5385 DBD LT recipients were included. 5620 DCD and 87561 DBD LT recipients were analyzed from the UNOS database. Meta‐analysis showed increased risk of patient mortality for DCD (HR = 1.36; 95%CI, 1.09–1.70; P = .01; I2 = 53.6%). When adjusted for publication bias, meta‐analysis showed no difference in patient survival between DCD and DBD (HR = 1.15; 95%CI, .91–1.45; P = .25; I2 = 61.5%). Meta‐analysis showed increased risk of graft loss for DCD (HR = 1.50; 95%CI, 1.20–1.88; P < .001; I2 = 67.8%). When adjusted for publication bias, meta‐analysis showed a reduction in effect size (HR = 1.36; 95%CI, 1.06–1.74; P = .02; I2 = 73.5%).ConclusionWhen adjusted for publication bias, no difference was identified between DCD and DBD regarding patient survival, while DCD was associated with an increased risk of graft loss.
The expression pattern of CD10 and CD31 identifies fine fibrovascular stroma of grade 1‐endometrial endometrioid carcinomas in cytology
Authors: Norimatsu Yoshiaki , Nishikawa Takeshi , Suzuki Hisae , Hosokawa Sho , Yano Hiroko , Maeda Yoshinobu , Kurokawa Tetsuji , Shinagawa Akiko , Kobayashi Tadao K. , Fulciniti Franco 
Published: October 2021

AbstractIntroductionThe objective of this study was to assess the diagnostic utility of CD10 in the differential diagnosis of grade 1‐endometrial endometrioid carcinoma (G1‐EEC) and the metaplastic changes associated with the endometrial glandular and stromal breakdown (EGBD) on liquid‐based cytological (LBC) samples.Methods(1) The type and distribution of CD10‐positive cells in EGBD and G1‐EEC patients were evaluated. (2) Based on the results from (1), histological and cytological specimens were double‐immunostained with CD31 and CD10 to confirm whether CD10‐positive tubular‐canalicular material found in (1) was represented by fine threads of endometrial‐type fibrovascular stroma. (3) Based on the results from (2), additional immunostaining of histological specimens was performed for CD146 and αSMA as markers of perivascular cells.Results(1) CD10 positive cells showed two main patterns of expression: cytoplasmic immunoreactivity in the form of dense brown granules in EGBD and tubular‐canalicular branching patterns in G1‐EEC. (2) The tubular‐canalicular material observed in cytological specimens of G1‐EEC samples co‐expressed CD10 and CD31, and was interpreted as representing fine threads of endometrial fibrovascular stroma in the corresponding histological samples. Conversely, metaplastic changes in EGBD cases, only a few CD31‐positive signals were found inside the condensed stromal clusters with CD10‐positive. (3) Cells surrounding the CD31‐positive vascular endothelial cells expressed CD146 and αSMA; moreover, some of the thin CD10‐positive fibrous stromal strands also co‐expressed αSMA.ConclusionsCD10 is a very useful immunomarker for distinguishing between G1‐EEC and the metaplastic changes of EGBD in LBC samples.
Growing cotton to produce food: Unravelling interactions between value chains in southern Mali
Authors: Dissa Arouna , Bijman Jos , Slingerland Maja , Sanogo Ousmane Mama , Giller Ken E , Descheemaeker Katrien 
Published: October 2021

AbstractMotivationMost transaction cost economic frameworks, commonly used to examine and explain the co‐ordination of agricultural transactions, use a linear approach for a single product transaction. This ignores the concurrence of multiple transactions by smallholder farmers in developing countries.PurposeThis study aims to understand co‐ordination among multiple product transactions by smallholder farmers and to identify ways to remove impediments to market participation. It develops an adapted transaction cost framework, considering contract types and forms of market participation as building blocks for co‐ordination structures.The framework was applied to explain co‐ordination structures between smallholders and buyers of cotton and cereals in southern Mali.Methods and approachTo make the framework operational, we did the following: (1) selected transaction characteristics; (2) elaborated benchmarks to describe the intensity of transactions; (3) identified co‐ordination structures; and (4) scored the intensity of transactions. Both quantitative and qualitative data were collected.FindingsThe majority of farmers grew cotton and sold it to a parastatal company, the sole buyer, that also supported the provision of inputs. Inputs were used to grow not only cotton, but also cereals. Most farmers sold cereals on spot markets to collectors and traders.Using different structures allowed smallholders to obtain inputs and services, to pursue different income sources over the year, and to balance flexibility and security.Policy implicationsCollective organizations of smallholder farmers should be supported to improve their financial and managerial capacities to allow them to co‐ordinate better with buyers and input suppliers.Institutional innovations to better balance risks for smallholders and buyers deserve consideration. These innovations include crop insurance, long‐term credit, and warehouse receipts.
Predictors of fever response in tuberculous meningitis: A clinical, MRI and biomarker study
Authors: Kalita Jayantee , Pandey Prakash C. , Shukla Ruchi , Haldar Rudrashish 
Published: October 2021

AbstractBackgroundCentral nervous system (CNS) has a different immune surveillance system; therefore, fever at admission and timeline of fever response after antitubercular treatment (ATT) may follow a different course in CNS infection. We report the predictors of fever response in tuberculous meningitis (TBM) including the effect of tumour necrosis factor‐α (TNF‐α) in cerebrospinal fluid (CSF) and its gene expression at mRNA of peripheral blood mononuclear cells (PBMCs).MethodsFifty‐seven patients with TBM were prospectively evaluated. Their clinical findings and severity of meningitis were recorded. The expression of TNF‐α gene in PBMCs was quantified by real‐time polymerase chain reaction and TNF‐α concentration in CSF by cytokine bead array both in the patients and 14 matched controls.ResultsAll the patients had history of fever for a median duration of 75 days. The admission temperature ranged between 37.2°C and 40°C and correlated with CSF cell counts (p < 0.05). Cranial MRI was abnormal in 54 (94.7%) and revealed exudates in 33(57.9%), hydrocephalus in 27(47.4%), infarction in 27(47.4%) and tuberculoma in 33(57.9%) patients. Fever subsided after a median duration of 18 (2 60) days of treatment. Twelve (21.8%) patients only became afebrile within 10 days. The expression of TNF‐α gene correlated with CSF concentration of TNF‐α (p = 0.02) and independently predicted duration of defervescence [adjusted hazard ratio 1.02 (95% CI 1.00–1.04; p = 0.01).ConclusionIn the patients with TBM, defervescence takes longer time, and TNF‐α gene expression predicts the duration of defervescence. Future studies are needed to evaluate the role of TNF‐α‐modifying drugs in TBM.
Surveillance and control of SARS‐CoV‐2 in mustelids: An evolutionary perspective
Authors: Díaz Adriana V. , Walker Martin , Webster Joanne P. 
Published: October 2021

AbstractThe relevance of mustelids in SARS‐CoV‐2 transmission has become increasingly evident. Alongside experimental demonstration of airborne transmission among ferrets, the major animal model for human respiratory diseases, transmission of SARS‐CoV‐2 within‐ and/or between‐commercial mink farms has occurred and continues to occur. The number of mink reared for the luxury fur trade is approximately 60.5 million, across 36 mustelid‐farming countries. By July 2021, SARS‐CoV‐2 outbreaks have been reported in 12 of these countries, at 412 European and 20 North American mink farms. Reverse zoonotic transmission events (from humans to mink) have introduced the virus to farms with subsequent extensive mink‐to‐mink transmission as well as further zoonotic (mink‐to‐human) transmission events generating cases among both farm workers and the broader community. Overcrowded housing conditions inherent within intensive mink farms, often combined with poor sanitation and welfare, both guarantee spread of SARS‐CoV‐2 and facilitate opportunities for viral variants, thereby effectively representing biotic hubs for viral transmission and evolution of virulence. Adequate preventative, surveillance and control measures within the mink industry are imperative both for the control of the current global pandemic and to mitigate against future outbreaks.
Predators mitigate the destabilising effects of heatwaves on multitrophic stream communities
Authors: Ross Samuel R. P.‐J. , García Molinos Jorge , Okuda Atsushi , Johnstone Jackson , Atsumi Keisuke , Futamura Ryo , Williams Maureen A. , Matsuoka Yuichi , Uchida Jiro , Kumikawa Shoji , Sugiyama Hiroshi , Kishida Osamu , Donohue Ian 
Published: October 2021

AbstractAmidst the global extinction crisis, climate change will expose ecosystems to more frequent and intense extreme climatic events, such as heatwaves. Yet, whether predator species loss—a prevailing characteristic of the extinction crisis—will exacerbate the ecological consequences of extreme climatic events remains largely unknown. Here, we show that the loss of predator species can interact with heatwaves to moderate the compositional stability of ecosystems. We exposed multitrophic stream communities, with and without a dominant predator species, to realistic current and future heatwaves and found that heatwaves destabilised algal communities by homogenising them in space. However, this happened only when the predator was absent. Additional heatwave impacts on multiple aspects of stream communities, including changes to the structure of algal and macroinvertebrate communities, as well as total algal biomass and its temporal variability, were not apparent during heatwaves and emerged only after the heatwaves had passed. Taken together, our results suggest that the ecological consequences of heatwaves can amplify over time as their impacts propagate through biological interaction networks, but the presence of predators can help to buffer such impacts. These findings underscore the importance of conserving trophic structure, and highlight the potential for species extinctions to amplify the effects of climate change and extreme events.
Impact of oral health guidance on the tongue‐lip motor function of outpatients at a dental hospital
Authors: Hatanaka Yukiko , Furuya Junichi , Sato Yuji , Uchida Yoshiki , Osawa Tokiko , Shichita Toshiharu , Suzuki Hiroyuki , Minakuchi Shunsuke 
Published: October 2021

AbstractObjectiveTo clarify the effect of oral health guidance on tongue‐lip motor function in the outpatients visiting the Showa University Dental Hospital (Tokyo, Japan).BackgroundThe management of the oral function of older people visiting a dental hospital is important. Previous studies have revealed that tongue‐lip motor function is easy to improve. However, the impact of oral health guidance on tongue‐lip motor function in the outpatients of dental hospital requires further elucidation.Materials and MethodsThe participants (n = 35) included patients who were diagnosed with low tongue‐lip motor function on evaluation by oral diadochokinesis (ODK) at the outpatient clinic. They underwent a second examination approximately 6‐12 months later. Their demographic characteristics were recorded. Oral health guidance was provided through an educational leaflet on oral hypofunction when the participants visited the clinic. It included content on tongue twisters, voice training and a range of movement and muscle training of the tongue and lip.ResultsFollowing oral health guidance on tongue‐lip motor function, the ODK values changed from 5.6 at the first examination to 6.0 at the second for /pa/, from 5.6 to 5.8 for /ta/ and from 5.2 to 5.4 for /ka/. This improvement was not significantly associated with age, sex, measurement period or number of visits.ConclusionThe findings of this longitudinal study suggested that oral health guidance using an oral hypofunction educational leaflet may be effective in improving the tongue‐lip motor function of outpatients who had low tongue‐lip motor function, regardless of the measurement period or the number of visits to the dental hospital.
Influence of heat treatment temperature on the properties of the lithium disilicate‐fluorcanasite glass‐ceramics
Authors: Kraipok Arnon , Intawin Pratthana , Bintachitt Patamas , Leenakul Wilaiwan , Khamman Orawan , Eitssayeam Sukum , Tunkasiri Tawee , Pengpat Kamonpan 
Published: October 2021

AbstractThis study aims to investigate the influence of heat treatment temperatures on the mechanical properties and chemical solubility (CS) of lithium disilicate‐fluorcanasite glass‐ceramics and to develop new dental materials. The glasses and glass‐ceramics were prepared using CaF2‐SiO2‐CaO‐K2O‐Na2O‐Li2O‐Al2O3‐P2O5‐based glass system using a conventional melt quenching method followed by a two‐stage crystallization process. This two‐stage method involves two heating temperature steps: first at a constant temperature (TS1) of 600°C and second step at varying temperatures (TS2) of 650, 700, 750, and 800°C. The crystallization behavior, phase formation, microstructure, translucency characteristic, density, hardness, fracture strength, and CS were investigated. It was found that the lithium disilicate crystal acted as the main crystalline phase, and the crystalline phase of fluorcanasite occurred at the heat treatment temperatures of 750 and 800°C. In addition, it was found that density, hardness, fracture strength, and CS increased while the translucency values decreased with increasing heat treatment temperatures. Furthermore, the CS increased dramatically when the fluorcanasite phases occurred in the glass‐ceramic samples. The maximum density values, Vickers hardness, fracture toughness, and flexural strength are 2.56 g/cm3, 6.73 GPa, 3.38 MPa.m1/2, and 259 MPa, respectively. These results may offer a possibility to design a new material for dental applications based on lithium disilicate‐fluorcanasite glass‐ceramics.
Lactiplantibacillus plantarum HG20 attenuates II type collagen-induced rheumatoid arthritis in rats via anti-inflammatory and inhibition of apoptosis
Authors: Liu Chunhong , Lin Li , Cui Weidong , Wang Lei , Ai Min , Zhao Zhongwei , Ma Xiaohan , Li Shengyu 
Published: October 2021

AbstractAimsThis study aimed to explore the therapeutic effects of Lactiplantibacillus plantarum HG20 (HG20) on collagen-induced arthritis (CIA) rats and its mechanism.Methods and ResultsCIA rats were established by injecting bovine type II collagen for 7 days, and treated by intragastric administration HG20 for 21 days. The foot palm temperature and arthritis score were measured once a week. The pathological changes in the knee joint were observed by hematoxylin and eosin staining. The levels of cytokines were detected by enzyme linked immunosorbent assay, and the effects of HG20 on inflammatory and apoptosis pathway of spleen cells were detected by western blot analysis. The results indicated that HG20 reduced the joint swelling degree and foot palm temperature, inhibited the development of joint histopathology, decreased the levels of pro-inflammatory cytokines, down-regulate the expression of pro-inflammatory cytokines by nuclear factor kappa-B pathway, and inhibited the apoptosis of spleen cells by inhibiting phosphatidylinositol 3-kinase/protein kinase B pathway and regulating apoptosis pathways.ConclusionsHG20 had an adjuvant therapeutic effect on arthritis in CIA rats, and its mechanism might be related to the inflammatory and apoptosis pathway.Significance and Impact of StudyThese results revealed that HG20 could be used as a functional probiotic in the field of food and medical, and which played a potential role in the prevention and treatment of arthritis.
Detection of virulence and extended spectrum β-lactamase genes in Salmonella by multiplex high-resolution melt curve real-time PCR assay
Authors: Dhital Rajiv , Shen Zhenyu , Zhang Shuping , Mustapha Azlin 
Published: October 2021

Abstract

Aims
Develop and standardize multiplex high-resolution melt curve (HRM) real-time PCR assays for simultaneous detection of Salmonella virulence and extended spectrum β-lactamase (ESBL) genes in food.

Methods and Results
Two sets of multiplex real-time PCR assays targeting six virulence and three ESBL genes with internal amplification control were standardized. The first assay detected hilA, fimH, sipA, blaTEM and blaSHV, and the second detected invA, fimA, stn and blaCMY. The PCR assays were validated with DNA samples from 77 different Salmonella strains. The assay specificity was tested with DNA from 47 non-Salmonella strains. Melt curve analyses showed distinct, well-separated melting peaks of each target gene detected by their respective melting temperatures (Tm). Different food samples were spiked with 10, 102 and 103 CFU/ml of Salmonella. The optimized assays were able to detect all target genes in concentrations of as low as 10 CFU/ml in 25 g foods within 10 h of enrichment.

Conclusions
Multiplex HRM real-time PCR assays can be used as rapid detection methods for detecting Salmonella in foods.

Significance and Impact of Study
The assays developed in this study will allow for accurate detection of virulence and ESBL genes in Salmonella that are present in low concentrations in food samples.
Ocular expression of cyclin‐dependent kinase 5 in patients with proliferative diabetic retinopathy
Authors: Sano Hiroki , Namekata Kazuhiko , Niki Masanori , Semba Kentaro , Murao Fumiko , Harada Takayuki , Mitamura Yoshinori 
Published: October 2021

ABSTRACTAims/IntroductionInhibition of peroxisome proliferator‐activated receptor gamma (PPARγ) phosphorylation mediated by cyclin‐dependent kinase 5 (Cdk5) is one of the main mechanisms of action of antidiabetic drugs. In this study, we analyzed the ocular expression and activation of Cdk5 in patients with proliferative diabetic retinopathy (PDR).Materials and MethodsThe concentrations of PPARγ, Cdk5 and its activating subunit (p35) were determined in the vitreous body of 24 PDR and 63 control eyes by enzyme‐linked immunosorbent assay. In addition, the messenger ribonucleic acid and protein expression levels of PPARγ, Cdk5 and p35 were measured in proliferative neovascular membranes from seven PDR eyes and non‐neovascular epiretinal membranes from five control eyes by quantitative real‐time polymerase chain reaction and immunohistochemical analysis.ResultsPPARγ, Cdk5 and p35 concentrations in the vitreous body were significantly higher in the PDR group compared with the control group. There was also a positive significant correlation of Cdk5 with PPARγ and p35 in the PDR group. Furthermore, the messenger ribonucleic acid expression levels of PPARγ, Cdk5 and p35 in proliferative neovascular membranes were significantly higher in the PDR group compared with the control group. Immunostaining showed increased protein expression levels of PPARγ, Cdk5 and p35 in proliferative neovascular membranes in the PDR group compared with the control group.ConclusionsCdk5 activation is involved in PDR pathogenesis through PPARγ expression, and inhibition of Cdk5‐mediated PPARγ phosphorylation might be a new therapeutic target for treatment of PDR.
The relation between narcissism and aggression: A meta‐analysis
Authors: Du Tianwei V. , Miller Joshua D. , Lynam Donald R. 
Published: October 2021

AbstractObjectiveNarcissism is a complex, hierarchical construct that can be studied at the one, two, or three factor levels with different components within each level having their own unique nomological networks. The manner in which narcissism—both broadly and narrowly construed—is linked to aggression is important to understand given longstanding clinical and empirical observations of a link between the two and the critical implications of aggression.MethodsThe current preregistered meta‐analysis (k = 118) took a novel methodological approach in exploring the association between the three levels of narcissism (i.e., global construct level, dual‐dimension level, trifurcated level) and three indices of aggression (i.e., general, proactive, reactive).ResultsResults revealed that the global construct of narcissism shows a moderate positive association with different indices of aggression. Vulnerable narcissism associated strongly and positively with reactive aggression and general aggression. At the trifurcated level, interpersonal antagonism associated positively with all indices of aggression, agentic extraversion associated negatively with all indices of aggression, and narcissistic neuroticism associates positively with general and reactive aggression.ConclusionThe study highlighted the importance of studying narcissism, and potentially other personality profiles, at a finer‐grained level to better understand crucial psychological processes associated with the construct of interest.
Coronavirus disease 2019 in kidney transplant recipients: a systematic review and meta-analysis
Authors: Ho Quan Yao , Sultana Rehena , Lee Tung Lin , Thangaraju Sobhana , Kee Terence , Htay Htay 
Published: October 2021

Introduction:
The clinical presentation and outcomes of coronavirus disease 2019 (COVID-19) in kidney transplant recipients (KTRs) have not been well studied.

Methods:
We performed a meta-analysis to examine the presenting features, outcomes and the effect of treatment on outcomes of KTRs with COVID-19. Database search was performed up to 5 September 2020 through PubMed, Embase, Web of Science, Scopus and CENTRAL.

Results:
Overall, 23 studies (1,373 patients) were included in the review and meta-analysis. The most common presenting symptoms included fever (74.0%, 95% confidence interval [CI] 65.3–81.1), cough (63.3%, 95% CI 56.5–69.6) and dyspnoea (47.5%, 95% CI 39.6–55.6). Pooled rates of mortality and critical illness were 21.1% (95% CI 15.3–28.4) and 27.7% (95% CI 21.5–34.8), respectively. Acute kidney injury occurred in 38.9% (95% CI 30.6–48.1) and dialysis was required in 12.4% (95% CI 8.3–18.0) of the cases.

Conclusion:
Kidney transplant recipients with COVID-19 have a similar clinical presentation as the general population, but they have higher morbidity and mortality. It is uncertain whether high-dose corticosteroid or hydroxychloroquine reduces the risks of mortality in KTRs with COVID-19.