bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thromboxane‐prostanoid receptor activation blocks ATP‐sensitive potassium channels in rat aortas
Authors: Santos Jeimison D. , Paulo Michele , Vercesi Juliana A. , Bendhack Lusiane M. 
Published: July 2021

AbstractK+ channel activation is one of the major mechanisms involved in vasodilation. Vasoconstrictor agonists such as angiotensin II promote ATP‐dependent potassium channels (KATP) dysfunction. This study evaluates whether thromboxane‐prostanoid (TP receptor) activation by the agonist U46619 increases reactive oxygen species (ROS) production in rat aortas, which could contribute to KATP channel dysfunction and impaired NO‐dependent vasodilation. TP receptor activation with the selective agonist U46619 increased ROS in endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), but the TP receptor antagonist SQ29548 abolished this effect. ECs and VSMCs incubation with ROS scavengers like Tiron or PEG‐Catalase impaired U46619‐induced ROS production. U46619 at the concentrations of 0.1 and 1 µmol/L induced contractions with similar amplitude. KATP channel activation with pinacidil‐induced relaxation was lower for the contractions induced with 0.1 or 1 µmol/L U46619 than with 10 nmol/L U46619. Acetylcholine‐induced relaxation provided similar results. In aortas pre‐contracted with 10 nmol/L U46619, neither Tiron (100 µmol/L) nor catalase (300 U/mL) affected pinacidil‐induced relaxation. However, in aortas pre‐contracted with 0.1 µmol/L U46619, catalase potentiated pinacidil‐induced relaxation. Pinacidil potentiated acetylcholine‐induced relaxation in aortas pre‐contracted with 0.1 and 1 µmol/L U46619. Incubation with 10 nmol/L U46619 increased NO concentration in ECs. Taken together, these results show that high concentrations of the TP receptor agonist U46619 impair KATP channels, which is probably due to ROS production. It is likely that hydrogen peroxide is the ROS.
Global co‐occurrence of methanogenic archaea and methanotrophic bacteria in Microcystis aggregates
Authors: Li Chuang , Hambright K. David , Bowen Hannah G. , Trammell Majoi A. , Grossart Hans‐Peter , Burford Michele A. , Hamilton David P. , Jiang Helong , Latour Delphine , Meyer Elisabeth I. , Padisák Judit , Zamor Richard M. , Krumholz Lee R. 
Published: July 2021

SummaryGlobal warming and eutrophication contribute to the worldwide increase in cyanobacterial blooms, and the level of cyanobacterial biomass is strongly associated with rises in methane emissions from surface lake waters. Hence, methane‐metabolizing microorganisms may be important for modulating carbon flow in cyanobacterial blooms. Here, we surveyed methanogenic and methanotrophic communities associated with floating Microcystis aggregates in 10 lakes spanning four continents, through sequencing of 16S rRNA and functional marker genes. Methanogenic archaea (mainly Methanoregula and Methanosaeta) were detectable in 5 of the 10 lakes and constituted the majority (~50%–90%) of the archaeal community in these lakes. Three of the 10 lakes contained relatively more abundant methanotrophs than the other seven lakes, with the methanotrophic genera Methyloparacoccus, Crenothrix, and an uncultured species related to Methylobacter dominating and nearly exclusively found in each of those three lakes. These three are among the five lakes in which methanogens were observed. Operational taxonomic unit (OTU) richness and abundance of methanotrophs were strongly positively correlated with those of methanogens, suggesting that their activities may be coupled. These Microcystis‐aggregate‐associated methanotrophs may be responsible for a hitherto overlooked sink for methane in surface freshwaters, and their co‐occurrence with methanogens sheds light on the methane cycle in cyanobacterial aggregates.
Cross‐cultural validation of the Turkish version of SCORE‐15
Authors: Işık Erkan , Günsel Damla Ayşegül , Stratton Peter 
Published: July 2021

AbstractThe purpose of the current study was to examine the validity and reliability of the fifteen‐item Systemic Clinical Outcome and Routine Evaluation (SCORE‐15) Turkish version. The suggested three‐factor structure was confirmed with a total sample of 327 participants including both community and clinical samples. The results of factorial invariance tests demonstrated that the structure of the scale was consistent across both gender and community/clinical groups. Furthermore, discriminant validity was supported by negative correlations with marital satisfaction, life satisfaction and general family functioning as lower scores in SCORE‐15 reflect better adjustment. Furthermore, results showed that SCORE‐15 has good internal consistency reliability and significantly differentiates between community and clinical samples. Overall, these findings indicate that the Turkish version of SCORE‐15 is a valid and reliable instrument that can be used for assessing family functioning in routine clinical practice and family therapy research.
Practitioner points

SCORE‐15 is a brief and effective instrument for assessing overall family functioning with specific dimensions of family strengths, communication and difficulties

The Turkish version of SCORE‐15 exhibited similar factorial properties across gender and community/clinical groups

The Turkish version of SCORE‐15 can be used in both clinical and community contexts for research and intervention
The development of the Reflecting Team Utterances Framework: Process, reflections and applications
Authors: Constable Chloe , Delaney Claire , Clutterham Adam , Markiewicz Abi 
Published: July 2021

AbstractThe Reflecting Team (RT) has been a common companion of systemic practitioners since Tom Andersen first described its use (Andersen, 1987). Despite the widespread acceptance of such ideas in modern Systemic Practice, there is limited research into the RT method. We hoped to contribute to the broader research aim of exploring how and why reflecting team conversations lead to change by addressing the following question: is it possible to reliably categorise the utterances of the RT to form a framework? We used a content analysis to analyse and categorise over three hours of reflecting team conversations from four different clinical teams. This resulted in the identification of eleven discrete categories including: ‘they asked a question to you as a family’, ‘they highlighted something positive and they commented on their own emotions’. We go on to discuss potential applications of this Reflecting Team Utterances Framework and our reflections on the process.
Practitioner points

The Reflecting Team Utterances (RTU) framework enables clinicians to facilitate discussion with trainees about the nature of a reflecting team and the categories of reflection and their potential for impact.

The framework may be a useful tool within teams as a way of reflecting on the utterances chosen by teams and to facilitate discussion around this. Questions such as “what are we most often saying and why” could add to supervisory discussions extending therapists’ repertories in reflecting and ensuring reflexivity regarding intentionality.

There is a potential to use the framework also with families, asking questions prior to reflecting teams such as ‘What feels most useful to you at this time; connections with our own experiences or suggestions about what you could experiment with?’
Pulmonary function, muscle strength, and quality of life have differed between chronic kidney disease patients and healthy individuals
Authors: de Souza Rezende Patrícia , Porcher Andrade Francini , Ferraro dos Santos Borba Carolina , Eidt Rovedder Paula Maria 
Published: July 2021

AbstractChronic kidney disease (CKD) patients have lower pulmonary function, respiratory and peripheral muscle strength values when compared to the general population, which reflects negatively in the quality of life (QoL). The aim of this study was to compare the pulmonary function, respiratory and peripheral muscle strength, and QoL between CKD patients and healthy individuals. Cross‐sectional study with a consecutive sample of 39 individuals separated into three groups: nondialysis‐dependent CKD in stage 5 (CKD‐5), HD group (CKD‐HD), and healthy individuals. The tests performed were spirometry, manovacuometry, handgrip strength (HGS), and Medical Outcomes Short‐Form Health Survey (SF‐36). A significant difference between groups was observed in forced expiratory volume in 1 second (FEV1) and its predicted value (p = 0.004 and p < 0.001, respectively), forced vital capacity (FVC), and its predicted value (p = 0.008 and p < 0.001, respectively), peak expiratory flow (PEF) and its predicted value (p = 0.004 and p < 0.001, respectively), maximal inspiratory pressure (MIP) and its predicted value (p = 0.022), maximal expiratory pressure (MEP) and its predicted value (p = 0.030 and p = 0.009, respectively) in which CKD‐5 presented the worst values, followed by CKD‐HD and healthy group. The CKD‐5 had worse pulmonary function, respiratory muscle strength, and QoL. Moreover, CKD‐HD also showed some impairment in pulmonary function and QoL when compared with healthy individuals. Even with the peripheral muscle strength preserved, its decrease is proportional to the pulmonary impairment in the evaluated CKD patients.
The future of our suburbs: Analyses of heatwave vulnerability in a planned estate
Authors: Haynes Victoria , Calgaro Emma , Dominey‐Howes Dale 
Published: July 2021

AbstractPlanned estates may be a solution to housing demand and affordability in high growth regions. As a result, planned estate research has tended to focus on privatisation, social engineering of communities by developers, and social polarisation. Heatwave research has focused on measuring and mitigating the impact of such events on disadvantaged communities. However, these two research streams have not been combined to examine how planned estate residents may be vulnerable to heatwaves. We address that gap by considering past, current, and future heatwave impacts; identifying underlying processes governing the vulnerability of planned estates to heatwaves; and reflecting on the implications for the sustainability of planned estates in relation to future heatwave risk. We adopt a mixed‐methods approach using the case of the Jordan Springs estate in Western Sydney, Australia, and that work is underpinned by systems approaches embedded in sustainability science. We find that Jordan Springs is physically vulnerable to heatwaves due to characteristics in its built environment. Human vulnerability is complex: rooted in multiscalar socio‐economic and systemic processes both internal and external to the estate. We suggest resilience be increased to improve estate liveability and sustainability by retrofitting the built environment, improving urban greening, broadening consumer awareness, and modifying development processes.
The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress
Authors: Llamas Ernesto , Torres‐Montilla Salvador , Lee Hyun Ju , Barja María Victoria , Schlimgen Elena , Dunken Nick , Wagle Prerana , Werr Wolfgang , Zuccaro Alga , Rodríguez‐Concepción Manuel , Vilchez David 
Published: July 2021

AbstractThe biological purpose of plant stem cells is to maintain themselves while providing new pools of differentiated cells that form organs and rejuvenate or replace damaged tissues. Protein homeostasis or proteostasis is required for cell function and viability. However, the link between proteostasis and plant stem cell identity remains unknown. In contrast to their differentiated counterparts, we find that root stem cells can prevent the accumulation of aggregated proteins even under proteotoxic stress conditions such as heat stress or proteasome inhibition. Notably, root stem cells exhibit enhanced expression of distinct chaperones that maintain proteome integrity. Particularly, intrinsic high levels of the T‐complex protein‐1 ring complex/chaperonin containing TCP1 (TRiC/CCT) complex determine stem cell maintenance and their remarkable ability to suppress protein aggregation. Overexpression of CCT8, a key activator of TRiC/CCT assembly, is sufficient to ameliorate protein aggregation in differentiated cells and confer resistance to proteotoxic stress in plants. Taken together, our results indicate that enhanced proteostasis mechanisms in stem cells could be an important requirement for plants to persist under extreme environmental conditions and reach extreme long ages. Thus, proteostasis of stem cells can provide insights to design and breed plants tolerant to environmental challenges caused by the climate change.
Is mandatory prospective trial registration working? An update on the adherence to the International Committee of Medical Journal Editors guidelines across five psychiatry journals: 2015–2020
Authors: Turner Eryn H. , Mulder Roger T. , Rucklidge Julia J. 
Published: July 2021

AbstractObjectivePrevious research indicated that the International Committee of Medical Journal Editors guidelines of prospective trial registration with clearly identified primary and secondary outcome measures are only adhered to in 14.4% of studies published in the top five psychiatry journals between 1 January 2009 and 31 July 2013. This study examined if adherence has improved.MethodsThe registration information, article information, primary outcome measures (POMs), participant numbers and funding source were extracted from studies published in the same five psychiatry journals between 1 January 2015 and 31 December 2019. Discrepancies between POMs in the articles and registry were tracked.ResultsOf the 7268 publications, 268 studies required registration. Three (1.1%) were unregistered, 107 (39.9%) were retrospectively registered, and 158 (58.9%) were prospectively registered. Of the 158 prospectively registered studies, 16 (10.1%) had unclear POMs in the article or registration, 22 (13.9%) had discrepancies between registered and published POMs, and 33 (20.9%) had no POM discrepancies but had retrospectively updated POMs in the registry. Of the 22 studies with discrepancies, nine (40.9%) were determined to favour statistically significant results. Overall, 87 (32.5%) of the 268 studies were prospectively registered with no discrepancies between registered and published POMs and no changes to registered POMs or timeframes.ConclusionAlthough this rate of one third of published articles fully adhering to the guidelines is an improvement compared to previous research, further efforts still need to be made by both authors and journals to ensure full transparency in the reporting of studies in psychiatry.
Intrahousehold distribution of sales revenue and household nutritional outcomes: What if the wives controlled the farm revenue?
Authors: Nikiema Relwendé A. , Sakurai Takeshi 
Published: July 2021

AbstractIn most developing countries, crop sales represent a means of improving the nutritional status of rural households. However, the expected effect of farm income may or may not be achieved, depending on who controls it. In this study, we use panel data on rural households in Burkina Faso to analyze the effect of the wives’ share of farm revenue on nutritional outcomes. The estimates show that raising wives’ share of farm revenue increases household food demand and nutrition quality. Moreover, the effect of the wives’ share of farm revenue on household nutrition is greater than the effect of the overall income per capita, which indicates that the intrahousehold distribution of farm income affects household nutritional status. We also show that increase in food expenditure and, more specifically, reallocation of the budget toward highly nutritious foods could represent a channel through which the control of farm revenue by women improves the household dietary quality. These findings imply that facilitating women's access to productive resources, such as land and labor‐saving technologies, could be the fastest way to improve household food and nutritional security.
Interstate protectionism: the case of solar renewable energy credits
Authors: Cohen Jed J. , Elbakidze Levan , Jackson Randall 
Published: July 2021

AbstractSolar Renewable Energy Credits (SRECs) are financial instruments created by state policies to offer incentives for generating solar energy. In an effort to support in‐state solar energy sectors and boost local employment opportunities, some states have closed off their SREC markets to out‐of‐state solar facilities. We examine the merits of such protectionist policy from the protectionist state's perspective. We find that SREC market closure leads to higher in‐state SREC prices and greater solar installation, and is associated with lower electricity prices. The study illustrates the economic incentives for protecting in‐state SREC markets from out‐of‐state solar energy producers.
Effect of hydroxychloroquine on the cardiac ventricular repolarization: A randomized clinical trial
Authors: Eveleens Maarse Boukje C. , Graff Claus , Kanters Jørgen K. , van Esdonk Michiel J. , Kemme Michiel J. B. , in 't Veld Aliede E. , Jansen Manon A. A. , Moerland Matthijs , Gal Pim 
Published: July 2021

AimsHydroxychloroquine has been suggested as possible treatment for severe acute respiratory syndrome‐coronavirus‐2. Studies reported an increased risk of QTcF‐prolongation after treatment with hydroxychloroquine. The aim of this study was to analyse the concentration‐dependent effects of hydroxychloroquine on the ventricular repolarization, including QTcF‐duration and T‐wave morphology.MethodsTwenty young (≤30 y) and 20 elderly (65–75 y) healthy male subjects were included. Subjects were randomized to receive either a total dose of 2400 mg hydroxychloroquine over 5 days, or placebo (ratio 1:1). Follow‐up duration was 28 days. Electrocardiograms (ECGs) were recorded as triplicate at baseline and 4 postdose single recordings, followed by hydroxychloroquine concentration measurements. ECG intervals (RR, QRS, PR, QTcF, J‐Tpc, Tp‐Te) and T‐wave morphology, measured with the morphology combination score, were analysed with a prespecified linear mixed effects concentration–effect model.ResultsThere were no significant associations between hydroxychloroquine concentrations and ECG characteristics, including RR‐, QRS‐ and QTcF‐interval (P = .09, .34, .25). Mean ΔΔQTcF‐interval prolongation did not exceed 5 ms and the upper limit of the 90% confidence interval did not exceed 10 ms at the highest measured concentrations (200 ng/mL). There were no associations between hydroxychloroquine concentration and the T‐wave morphology (P = .34 for morphology combination score). There was no significant effect of age group on ECG characteristics.ConclusionIn this study, hydroxychloroquine did not affect ventricular repolarization, including the QTcF‐interval and T‐wave morphology, at plasma concentrations up to 200 ng/mL. Based on this analysis, hydroxychloroquine does not appear to increase the risk of QTcF‐induced arrhythmias.
Making a Constituent Assembly Possible in Chile: The Shifting Costs of Opposing Change
Authors: Escudero Maria Cristina 
Published: July 2021

On 25 October 2020, Chile voted in favour of a Constitutional Convention (or Constituent Assembly) to replace the 1980 constitution. This kind of constituent mechanism had previously been resisted by the traditional political parties, most of whom now support the mechanism. This article identifies the political groups who opposed a Constituent Assembly and argues that the shift in approach was due to three factors. First, there had been a cumulative process of social pressure and political reactions since 2006. Second, the social unrest of October 2019 raised costs for the political elite – right and left – of denying a Constituent Assembly. Third, the establishment was able to reduce the uncertainty of the constitution‐making process.
Review: Universal mental health interventions for young students in adverse environments – a systematic review of evaluated interventions
Authors: Higgen Sanna , Mueller Jessica T. , Mösko Mike 
Published: July 2021

BackgroundUniversal classroom‐based interventions are a useful method to increase the mental health and resilience in children. Resilience describes the process that leads to a positive development despite adversities. It comprises the seven resources access to material resources, relationships, identity, power and control, cultural adherence, social justice and cohesion. Yet there is a paucity of studies evaluating interventions that enhance resilience in children exposed to adverse childhood experiences.MethodThis systematic review investigates whether universal classroom‐based interventions can increase the seven resilience‐related resources in children that live in adverse environments. Search strings were formulated based on an adapted version of the PICO criteria. The risk of bias of the individual studies was assessed using the ROBINS‐I tool.ResultsSeventeen studies were included in the review, of which 15 found an increase in resilience. The resource power and control was targeted in every intervention. Not one intervention included all seven resources. Intervention outcomes related mostly to just two of the resources (power and control and identity) and were rarely linked to what was being trained in the intervention.ConclusionThe results of this review show that classroom‐based interventions are suitable for promoting resilience in children living in adverse environments. Yet more high‐quality studies are needed that evaluate the effectiveness of universal interventions on children living in adverse environments and specifically the effectiveness of training each of the seven resources. Future developments of school‐based interventions should be careful to target and assess all resilience‐related resources.
Catalytic mechanism of butane anaerobic oxidation for alkyl‐coenzyme M reductase
Authors: Tian Xiaopian , Liu Hao , Chen Hai‐Feng 
Published: July 2021

AbstractMethane is among the most potent of the greenhouse gases, which plays a key role in global climate change. As an excellent carbon and energy source, methane can be utilized by anaerobic methane oxidizing archaea and aerobic methane oxidizing bacteria. The previous work shows that an anaerobic thermophilic enrichment culture composed of dense consortia of archaea and bacteria apparently uses partly similar pathways to oxidize the C4 hydrocarbon butane. However, the catalytic mechanism of butane anaerobic oxidation for alkyl‐coenzyme M reductase is still unknown. Therefore, molecular dynamics (MD) simulation was used to investigate the dynamics differences of catalytic mechanism between methane coenzyme M reductase (MCR) and alkyl‐coenzyme M reductase (ACR). At first, the binding pocket of ACR is larger than that of MCR. Then, the complex of butane and ACR is more stable than that of methane and ACR. Protein conformation cloud suggests that the position of methane is dynamics and methane escapes from the binding pocket of ACR during most of the simulation time, while butane tightly binds in the pocket of ACR. The hydrophobic interactions between butane and ACR are more and stronger than those between methane and ACR. At the same time, the binding free energy between butane and ACR is significantly lower than that between methane and ACR. The dynamics correlation network indicates that the transformation of information flow for ACR‐butane is smoother than that for ACR‐methane. The shortest pathway for ACR‐butane is from Gln144, Ala141, Hie135, Ile133, Ala160, Arg206, Asp97, Met94, Tyr347 to Phe345 with synergistic effect for two butane molecules. This study can insight into the catalytic mechanism for butane/ACR complex.