bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection
Authors: De Biasi Sara , Tartaro Domenico Lo , Gibellini Lara , Paolini Annamaria , Quong Andrew , Petes Carlene , Awong Geneve , Douglas Samuel , Lin Dongxia , Nieto Jordan , Borella Rebecca , Fidanza Lucia , Mattioli Marco , Leone Chiara , Meschiari Marianna , Franceschini Erica , Cicchetti Luca , Trenti Tommaso , Sarti Mario , Girardis Massimo , Guaraldi Giovanni , Mussini Cristina , Facchinetti Fabio , Cossarizza Andrea 
Published: March 2021

Abstract
In 14 pregnant women who had asymptomatic or paucisymptomatic SARS-CoV-2 infection, we performed a detailed 38-parameter analysis of peripheral blood mononuclear cells by mass cytometry, studied the expression of T-cell master regular genes, investigated cell proliferation and cytokine production, and measured plasma levels of 62 cytokines. No patient showed lymphopenia or gross alterations of white blood cells. Unsupervised analyses revealed that most immune parameters were similar in patients and uninfected controls, apart from an increase in low density neutrophils in SARS-CoV-2 positive women. Also, patients did not show altered plasma levels of interleukin-6 or other main inflammatory molecules, but displayed significant increases of anti-inflammatory cytokines such as IL-1RA, IL-10 and IL-19, and decreased levels of IL-17, PD-L1 and D-dimer. The endogenous control of inflammation, as evidenced by plasma levels of soluble molecules, could be a strategy used during pregnancy to avoid virus-induced damages and maintain a normal immune response.
The Baltimore Urban food Distribution (BUD) App: Study Protocol for A Food Systems Intervention
Authors: Gittelsohn Joel , Lewis Emma C. , Martin Nina M. , Zhu Siyao , Poirier Lisa , Dongen Ellen van , Ross Alexandra , Rex Samantha M. , Labrique Alain B. , Reznar Melissa M. , Igusa Takeru , Trujillo Antonio J. 
Published: March 2021

Abstract
Background: Low-income urban communities in the United States commonly lack ready access to healthy foods. This is due in part to a food distribution system that favors the provision of high-fat, high-sugar, high-sodium processed foods to small retail food stores, and impedes their healthier alternatives, such as fresh produce. Methods: The Baltimore Urban food Distribution (BUD) study is a multi-level, multi-component systems intervention that aims to improve healthy food access in low income neighborhoods of Baltimore, Maryland. The primary intervention is the BUD application (app), which uses the power of collective purchasing and delivery to affordably move foods from local producers and wholesalers to the city’s many corner stores. We will implement the app in a sample of 38 corner stores, randomized to intervention and comparison. Extensive evaluation will be conducted at each level of the intervention to assess effectiveness via process and impact measures on suppliers, corner stores, and consumers. Discussion: BUD represents one of the first attempts to implement an intervention that engages multiple levels of a local food system. The design of the intervention and the evaluation plan of the BUD project are documented here.Trial registration #: #### (register since not yet recruiting)
Detecting Potentially Ineffective Care in Critically Ill Patients in a Community Hospital: Assessing Sustained Predictive Value after Two Decades
Authors: Fleegler Bruce , Grimes Cindy , Bass Caitlin 
Published: March 2021

Abstract
Background: Eighteen years ago, we derived a formula to predict 100-day post-discharge mortality, utilizing the Acute Physiology and Chronic Health Evaluation III (APACHE III) data. This study was designed to reassess this formula when applied to a new cohort of patients, utilizing the updated predictive hospital mortality equations derived from APACHE IV.Methods: Compared with the 1995‒1997 cohort in our original study, this study included a cohort of intensive care unit patients from 2012‒2017, with similar demographics. Both cohorts included patients >18 years old admitted to and surviving at least five days of intensive care in the Sarasota Memorial Hospital in Sarasota, Florida, USA. Results: In the recent cohort, the formula exhibited a specificity of 99.7%, sensitivity of 17.8%, false positive rate of 0.3%, and positive predictive value of 92.6%; applied to the original cohort, the formula exhibited values of 98.7%, 33.8%, 1.3%, and 93.3%, respectively. There was no statistical difference between the two databases, except in sensitivity. Conclusions: Potentially ineffective care can be predicted with nearly the same specificity and predictive value using the formula developed in the 2002 study. If these results are reproducible at other institutions, they could assist in patient/family and palliative care discussions.
LEDitSHAKE – A Lighting System to Optimize the Secondary Metabolite Content of Plant Cell Suspension Cultures
Authors: Beuel Ann-Katrin , Jablonka Natalia , Heesel Julia , Severin Kevin , Spiegel Holger , Rasche Stefan 
Published: March 2021

Abstract
1.1 Background
Plant secondary metabolites are widely used in the food, cosmetic and pharmaceutical industries. They can be extracted from naturally grown plants or plant cell suspension cultures grown under sterile conditions. In the latter case, yields and quality strongly depend on the cultivation environment, including optimal illumination. Current shaking incubators do not allow different light wavelengths, intensities and photoperiods to be tested in parallel. We therefore developed a system for multiplexed customized illumination within a single shaking incubator, and used it to optimize anthocyanin production in grapevine cell suspension cultures.
1.2 Results
We used 3D printing to integrate light-emitting diode assemblies into flask housings to develop the LEDitSHAKE system, allowing 12 different lighting conditions (spectrum, intensity and photoperiod) to be tested simultaneously in a single shaking incubator. We used a Design of Experiments approach to determine the effect of 24 different light compositions on the total anthocyanin content of replicate grapevine cell suspension cultures. All tested conditions achieved higher yields than standard illumination or dark cultivation, and the optimal spectrum (8.3 µmol m− 2 s− 1 red, 8.3 µmol m− 2 s− 1 green, 33.3 µmol m− 2 s− 1 blue, and UV turned on) increased the total anthocyanin concentration by 2.42-fold after 4 weeks. Based on the resulting model, we predicted the optimal lighting conditions for the upregulation and downregulation of 30 anthocyanins, and found that short-wavelength light (blue, UV) maximized the concentration of most anthocyanins, whereas long-wavelength light (red) had the opposite effect. For example, the cyanidin glucoside concentration was predicted to increase 2.99-fold compared to the source culture with optimized illumination (12 µmol m− 2 s− 1 green, 38 µmol m− 2 s− 1 blue, and UV turned on for 1 h/day).
1.3 Conclusions
The LEDitSHAKE system enables the screening of up to 12 different lighting conditions in terms of spectrum, intensity and photoperiod within a single shaking incubator using a Design of Experiments approach, as exemplified by the optimization of the anthocyanin content and composition in grapevine cell suspension cultures. Our results demonstrate proof of principle that the LEDitSHAKE system is suitable for the optimization of processes based on plant cell suspension cultures.
Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation
Authors: Benjamin Sarah , Hawley Kelly L. , Vera-Licona Paola , Vake Carson J. La , Cervantes Jorge L. , Ruan Yijun , Radolf Justin D. , Salazar Juan C. 
Published: March 2021

Abstract
Background: Macrophages play prominent roles in bacteria recognition and clearance, including Borrelia burgdorferi (Bb), the Lyme disease spirochete. To elucidate mechanisms by which MyD88/TLR signaling enhances clearance of Bb by macrophages, we studied wildtype (WT) and MyD88-/- Bb-stimulated bone marrow-derived macrophages (BMDMs). Results: MyD88-/- BMDMs exhibit impaired uptake of spirochetes but comparable maturation of phagosomes following internalization of spirochetes. RNA-sequencing of infected WT and MyD88-/- BMDMs identified a large cohort of differentially expressed MyD88-dependent genes associated with re-organization of actin and cytoskeleton during phagocytosis along with several MyD88-independent chemokines involved in inflammatory cell recruitment. We computationally generated networks which identified several MyD88-dependent intermediate proteins (Rhoq and Cyfip1) that are known to mediate inflammation and phagocytosis respectively. Conclusion: Our findings show that MyD88 signaling enhances, but is not required, for bacterial uptake or phagosomal maturation and provide mechanistic insights into how MyD88-mediated phagosomal signaling enhances Bb uptake and clearance.
Description of the use of Multicriteria to Support Pricing and Reimbursement Decisions by European Health Technology Assessment Bodies.
Authors: Elvira David , Obach Mercè , Pontes Caridad 
Published: March 2021

Abstract
BackgroundHeterogeneity in drug access throughout Europe may be influenced by differences in drug-assessment strategies. The EUnetHTA’s assessment core model (EUnetHTA-core) and the EVIDEM’s multicriteria framework are reference methodologies in this context, the latter including a wider compromise between non-contextual and contextual criteria. Compliance of 37 European Health Technology Assessment bodies (HTAb) with EUnetHTA-core has been reported, but the use of EVIDEM by this HTAb is still unknown.ObjectiveTo describe the uptake and use of multicriteria approaches to evaluate drug value by European HTAb using EVIDEM as reference framework. MethodsMulticriteria framework was obtained based on EVIDEM model. The criteria used for drug appraisal by HTAb was extracted from the EUnetHTA report, and completed through search of websites, publications and HTAb reports. Use of EVIDEM assessment model in 37 European HTAb has been described semi-quantitatively and summarized using an alignment heatmap.ResultsAligned, medium or misaligned profiles were seen for 24,3%, 51,4% and 24,3% of HTAb when matching to EVIDEM dimensions and criteria was considered. HTAb with explicit responsibilities in providing specific advice on reimbursement showed more aligned profiles on contextual and non-contextual dimensions. ConclusionsMost of the 37 European HTAb have room to broaden their contextual assessment tools, especially when social and medical perception of need requires to be explicit to support payer’s decision on reimbursement.
Multidisciplinary residential home intervention to improve outcomes for frail residents
Authors: Steel Anna , Hopwood Helen , Goodwin Elizabeth , Sampson Elizabeth L 
Published: March 2021

Abstract
BackgroundResidential homes provide accommodation and assistance with personal care only and are not required to have registered nurses on site. However, their residents often have a combination of comorbidity, polypharmacy, frailty and mental-health conditions with poor access to healthcare to meet these needs. Integrated healthcare for older people is a key NHS priority in the Long-Term Plan and the Five-Year Forward View. We describe development and implementation of multi-disciplinary intervention to integrate healthcare and promote interprofessional education.MethodsA multi-disciplinary residential home quality improvement project in two cycles by a team comprising senior and trainee general practitioners, trainees in geriatrics, psychiatry, pharmacist and residential home senior staff. The intervention was underpinned by the framework for enhanced health in care homes including Comprehensive Geriatric Assessment (CGA) and mental-health review. Each intervention session included an educational presentation by a team member consideration of each resident in a pre-evaluation multi-disciplinary discussion followed by a structured clinical assessment and discussion of proposed management.ResultsThree residential homes participated with a total 34 residents receiving intervention. In one residential home, there was a 75% reduction in admissions for those reviewed and a reduction in overall admission costs. Polypharmacy was reduced by an average of 2 medications per resident across the three sites. There was a 63% increase in cardio-pulmonary resuscitation decisions and 76% increase in advance care planning discussions.ConclusionThis was an effective model for multi-disciplinary trainees working with a perceived impact on physical and mental health, and valuable opportunities for sharing learning.
Optimal Booking Control for Physical Examination considering Late Cancellation and Overbooking
Authors: Ho Te-Wei , Kung Ling-Chieh , Lai Jui-Fen , Chiu Han-Mo 
Published: March 2021

Abstract
Background: Late cancellation of physical examination has a severe impact on the profit of a healthcare center since it is often too late to ll the vacancy. A booking control policy that considers overbooking is then one natural solution.Case presentation: In this study, we consider a healthcare center providing different examination sets using dierent resources. As each resource has its unique cost, revenue, and capacity, the optimal booking limits of all examination sets are hard to be calculated. We propose a probabilistic optimization model that maximizes the expected prot given the late cancellation probability of each type of customer, where the probabilities are estimated through logistic regression and customer grouping using historical booking and cancellation records. To test the performance of our proposed solution, we collaborate with a leading healthcare center. We simulate the presence and absence of customers generated by historical records and compare different strategies of overbooking.Conclusions: Through the experiment, we show that our method can significantly increase the expected profit of the healthcare center by around 11%.
HPV Positivity and Risk of Cervical Cancer according to HIV-Infection: A Comparative Assessment in the Population of Cameroonian Women
Authors: SOSSO Samuel Martin , TCHOUAKET Michel Carlos TOMMO , Fokam Joseph , Simo Rachel Kamgaing , Semengue Ezechiel Ngoufack Jagni , Sando Zacharie , Torimiro Judith , Tiga Aline , Lobe Elise Elong , Ambada Georgia , Nange Achille , Nka Alex Durand , Tala Valère , Chenwi Collins , ABBA Aissatou , Ka’e Aude Christelle , Fainguem Nadine , Zam Marie Krystel Nnomo , Yagai Bouba , Billong Serge Clotaire , Colizzi Vittorio , Ndjolo Alexis 
Published: March 2021

Abstract
Background

Cervical cancer, caused by the human papillomavirus (HPV), remains a global health challenge. In HIV highly-burdened settings, it would be relevant to understand the severity of cervical cancer in case of co-infection with HPV. We therefore sought to determine the effect of HPV on the occurrence of cervical lesions among women with versus without HIV-infection.
Methods

A cross-sectional analytical study was conducted throughout 2012 among 257 women living in Yaoundé, Cameroon. HIV serology, genotyping of high-risk oncogenic HPV and cervical vaginal smear (CVS) were performed for all participants; among those reported to be HIV seropositive, HIV plasma viral load and CD4 count were measured. Results of the CVS were interpreted following the Bethesda 2001 guidelines. Statistical analyses were performed using Graph Pad version 6.0; p < 0.05 was considered statistically significant.
Results

The mean age of our study participants was 37 ± 6.5 years. According to HIV serology, 184 (71.59%) were HIV-positive vs. 73 (28.40%) HIV-negative women, with a similar age distribution respectively (36 ± 2.80 years versus 42 ± 8.48 years). Among HIV-positive women, median CD4 was 438 [IQR: 317–597] cells/mm3 and median viremia < 40 [IQR: <40 − 2318] copies/mL. Following successful genotyping, the prevalence of high-risk oncogenic HPV was 36.32% (73/201), with a significantly higher proportion among those with HIV-infection (41.98% [55/131] vs. 25.71% [18/70]; p = 0.02; OR = 2.1). CVS revealed 31.74% (97) normal cervix; 38.91% (100) inflammation; 16.34% (42) low-grade squamous intra-epithelial lesion; 6.34% (18) high-grade squamous intra-epithelial lesion. Overall rate of cervical lesions was 23.34% (60/257), with a non-significantly higher proportion in HIV-infected participants (25.00% [46/184] versus 19.17% [14/73]; p = 0.31). Of relevance, the presence of high-risk oncogenic HPV was significantly associated with cervical lesions (p < 0.0001; OR = 5.07), with a higher risk of cervical lesion among HIV-positive (p < 0.0001 and OR = 5.67) versus HIV-negative (p = 0.03 and OR = 3.83).
Conclusion

Though oncogenic HPV appears as an independent factor of the occurrence of cervical lesions, the risk of cervical lesion is substantially higher among HIV/HPV co-infection compared to HPV-infection alone. Thus, prevention of cervical cancer should be prioritised for women living with HIV-infection in HPV-endemic settings.
Social Media and Emotional Burnout Regulation During the COVID-19 Pandemic: Multilevel Approach
Authors: Shao Ruosi , Shi Zhen , Zhang Di 
Published: March 2021

Background
In February 2020, the Chinese government imposed a complete lockdown of Wuhan and other cities in Hubei Province to contain a spike of COVID-19 cases. Although such measures are effective in preventing the spread of the virus, medical professionals strongly voiced a caveat concerning the pandemic emotional burnout at the individual level. Although the lockdown limited individuals’ interpersonal communication with people in their social networks, it is common that individuals turn to social media to seek and share health information, exchange social support, and express pandemic-generated feelings.

Objective
Based on a holistic and multilevel perspective, this study examines how pandemic-related emotional exhaustion enacts intrapersonal, interpersonal, and hyperpersonal emotional regulation strategies, and then evaluates the effectiveness of these strategies, with a particular interest in understanding the role of hyperpersonal-level regulation or social media–based regulation.

Methods
Using an online panel, this study sampled 538 Chinese internet users from Hubei Province, the epicenter of the COVID-19 outbreak in China. Survey data collection lasted for 12 days from February 7-18, 2020, two weeks after Hubei Province was placed under quarantine. The sample had an average age of 35 (SD 10.65, range 18-78) years, and a majority were married (n=369, 68.6%).

Results
Using structural equation modeling, this study found that intrapersonal-level (B=0.22; β=.24; P<.001) and interpersonal-level (B=0.35; β=.49; P<.001) emotional regulation strategies were positively associated with individuals’ outcome reappraisal. In contrast with intrapersonal and interpersonal regulations, hyperpersonal (social media–based) regulation strategies, such as disclosing and retweeting negative emotions, were negatively related to the outcome reappraisal (B=–1.00; β=–.80; P<.001).

Conclusions
Consistent with previous literature, intrapersonal-level regulation (eg, cognitive reappraisal, mindfulness, and self-kindness) and interpersonal-level supportive interaction may generate a buffering effect on emotional exhaustion and promote individuals’ reappraisal toward the stressful situation. However, hyperpersonal-level regulation may exacerbate the experienced negative emotions and impede reappraisal of the pandemic situation. It is speculated that retweeting content that contains pandemic-related stress and anxiety may cause a digital emotion contagion. Individuals who share other people’s negative emotional expressions on social media are likely to be affected by the negative affect contagion. More importantly, the possible benefits of intrapersonal and interpersonal emotion regulations may be counteracted by social media or hyperpersonal regulation. This suggests the necessity to conduct social media–based health communication interventions to mitigate the social media–wide negative affect contagion if lockdown policies related to highly infectious diseases are initiated.
Seed and Germination Study of a New Guinea Endemic Plant Species <i>Grevillea papuana</i> Diels.
Authors: Kuswantoro Farid 
Published: March 2021

Grevillea papuana is a culturally important endemic plant species of New Guinea highland. Although G. papuana conservation and propagation attempts were already conducted, this species seed and germination characters information were still very limited. This study aimed to provide information regarding G. papuana seed and germination biology. Seed characteristic and germination trials were conducted in this study. Data analysis was conducted descriptively, while germination parameters were also calculated. G. papuana has a light, elliptic, and winged seed. The species germination was low and ununiform with phanerocotylar epigeal foliaceous (PEF) seedling functional type.
A STUDY ON DRUG UTILIZATION EVALUATION AND POTENTIAL DRUG RELATED PROBLEMS IN TYPE-2 DIABETES MELLITUS PATIENTS WITH CO-MORBIDITIES IN A TERTIARY CARE HOSPITAL
Authors: RODRIGUES PRUDENCE A. , DAMIEN DAWN TESLIN , K. T. MARY CECIL , M. NARESH KUMAR , S. S. RESHMA , KUMAR R. SENTHIL 
Published: March 2021

Objective: The objective of this study is to evaluate prescribing pattern, analyze drug-related problems, identify co-morbidities and complications in Type 2 Diabetes Mellitus patients and also to perform cost analysis of Biguanides, Sulfonylureas, Dipeptidyl Peptidase Inhibitors
Methods: A Prospective observational study was done in General Medicine and Endocrinology Departments of PSG Hospitals. A total of 200 study subjects, including both inpatients and outpatients, above 18 y of age, prescribed with OHAs (Biguanides, Sulfonylureas and DPP4 inhibitors) were included in the study.
Results: Diabetes was more prevalent among males. OHAs were most prescribed in the age group of 51-60 y. Hypertension (71%) was the most common comorbidity and Diabetic neuropathy (23%) was the most common complication found in the patients. Multidrug therapy (72.5%) was most prescribed in diabetic patients, followed by Biguanides. Hypoglycemia was the most prevalent ADR. Cost analysis showed that T. Linagliptin was of high cost and T. Glimepiride being the low cost. Using WHO core indicators prescribing patterns were assessed. Feedback was collected and results were reported to the physicians which showed rational utilization of drugs.
Conclusion: The study on drug utilization conducted in a tertiary care hospital helped us to find out that prescribing trends seems to be progressing towards combination therapy, predominantly two-drug therapy