bluuebunny/crossref_metadata_2025_split huggingface dataset:
PIWI-interacting RNA 57125 restrains clear cell renal cell carcinoma metastasis by downregulating CCL3 expression
Authors: Ding Lifeng , Wang Ruyue , Xu Wanjiang , Shen Danyang , Cheng Sheng , Wang Huan , Lu Zeyi , Zheng Qiming , Wang Liya , Xia Liqun , Li Gonghui 
Published: November 2021

AbstractClear-cell renal cell carcinoma is one of the most common tumors disagnosed, with nearly one third of patients diagnosed with metastatic ccRCC. Although an increasing number of studies has revealed that piwi-interacting RNAs are aberrantly expressed in diverse types of cancers, few of them explored the detailed molecular mechanism of piRNAs in carcinogenesis, particularly in ccRCC. In this study, differentially expressed piRNAs associated with ccRCC were selected by using piRNA-sequencing combined with TCGA data analysis, and piR-57125 was identified. PiR-57125 was found remarkably downregulated in ccRCC samples. Functionally, knockdown of piR-57125 promoted migration and invasion of ccRCC, while overexpression of piR-57125 suppressed ccRCC metastasis. In vivo lung metastasis model also confirmed the same results. CCL3 was identified as the direct target of piR-57125 which could potentially reverse the inhibition effect of piR-57125 in ccRCC metastasis. Further study revealed that piR-57125 modulated ccRCC metastasis through the AKT/ERK pathway. These data indicate that piR-57125 restrains ccRCC metastasis by directly targeting CCL3 and inhibiting the AKT/ERK pathway, and could be a potential therapeutic target for ccRCC.
GSK-3β manipulates ferroptosis sensitivity by dominating iron homeostasis
Authors: Wang Lingjuan , Ouyang Sijin , Li Bin , Wu Hao , Wang Fengli 
Published: November 2021

AbstractFerroptosis is a newly characterized form of non-apoptotic-programmed cell death, which is driven by the lethal accumulation of iron-catalyzed lipid peroxides. Uncontrolled ferroptosis is implicated in the pathogenesis of a group of human diseases, while targeted induction of ferroptosis provides a potent therapeutic design for cancers. During the past decade, the fundamental regulatory circuits of ferroptosis have been identified. In this study, we show that the multifaceted Ser/Thr protein kinase GSK-3β acts as a positive modulator of the ferroptosis program. Pharmacological inhibition of GSK-3β by selective inhibitor LY2090314 or genetic KD of GSK-3β by shRNA potently promotes ferroptotic resistance. GSK-3β KD antagonizes the expression of iron metabolic components including DMT1, FTH1, and FTL, leading to the disruption of iron homeostasis and decline in intracellular labile free iron level. Taken together, our findings elaborate an indispensable role of GSK-3β in determining ferroptotic sensitivity by dominating cellular iron metabolism, which provides further insight into GSK-3β as a target for cancer chemotherapy.
Towards a self tuning sliding mass metastructure
Authors: Bukhari Mohammad A. , Barry Oumar R. 
Published: November 2021

AbstractPassive vibration control systems are characterized by their simple practical design and independence of external power supplies. However, they are usually hindered by their narrow frequency band that cannot handle variable frequency disturbances. Recent research has demonstrated the capability of passive self-tuning resonators through the use of a sliding mass without the need for any external power sources. This work analytically and experimentally investigates the passive self-tuning of a metastructure consisting of a clamped-clamped beam with a sliding mass. The governing equations of motion show that the slider can be driven by Coriolis and centrifugal forces upon applying the excitation force on the structure. To improve the accuracy of our analytical simulations, we derive the exact instantaneous mode shapes and frequencies of the structure and feed them into an adaptive algorithm, which updates the spatial state of the system. Numerical simulations demonstrate that the proposed resonator can tune itself to the excitation frequency as the slider reaches the equilibrium position. This observation suggests that a significant vibration reduction can be obtained using the proposed resonator over a wide frequency band. Experiments are carried out to validate the analytical findings. The proposed structure can be used in different vibration control applications (i.e., aerospace, automotive, and machining), and its model can further be extended to self-adaptive periodic structures (metamaterials).
A Randomized Controlled Trial of the Effects of Group Conversation Treatment on Monologic Discourse in Aphasia
Authors: Hoover Elizabeth , DeDe Gayle , Maas Edwin 
Published: November 2021

Purpose:
Evidence has shown that group conversation treatment may improve communication and reduce social isolation for people with aphasia. However, little is known about the impact of conversation group treatment on measures of discourse. This project explored the impact of conversation treatment on measures of monologic discourse.

Method:
In this randomized controlled trial, 48 participants with chronic aphasia were randomly assigned to dyadic, large group, or control conditions. Conversation group treatment was provided for 1 hr, twice per week, for 10 weeks. Discourse samples were collected and coded at pretreatment, posttreatment, and 6-week maintenance. There were three narrative tasks: (a) Comprehensive Aphasia Test (CAT) picture description, (b) Cat Rescue Picture, and (c) Cinderella retell. All narratives were coded using the percent correct information units (percent CIUs), the CAT standardized narrative analysis method, and the complete utterance (CU) method.

Results:
No significant changes were observed on percent CIU, which was the primary outcome measure. The treated groups demonstrated improvement on aspects of the CU method following treatment, whereas the control group did not. Significant changes were observed for other CIU measures and the CAT standardized narrative analysis in both the treated and control groups.

Conclusions:
The results suggest that the CU measures were more sensitive to the effects of conversation treatment in monologic discourse compared to CIU and CAT measures. Changes were more common in absolute rather than relative values, suggesting that conversation treatment impacts the overall amount of language produced rather than efficiency of production.
Developing an Analytical Pipeline to Classify Patient Safety Event Reports Using Optimized Predictive Algorithms
Authors: Adadey Asa , Giannini Robert , Possanza Lorraine B. 
Published: October 2021

Abstract
Background Patient safety event reports provide valuable insight into systemic safety issues but deriving insights from these reports requires computational tools to efficiently parse through large volumes of qualitative data. Natural language processing (NLP) combined with predictive learning provides an automated approach to evaluating these data and supporting the work of patient safety analysts.
Objectives The objective of this study was to use NLP and machine learning techniques to develop a generalizable, scalable, and reliable approach to classifying event reports for the purpose of driving improvements in the safety and quality of patient care.
Methods Datasets for 14 different labels (themes) were vectorized using a bag-of-words, tf-idf, or document embeddings approach and then applied to a series of classification algorithms via a hyperparameter grid search to derive an optimized model. Reports were also analyzed for terms strongly associated with each theme using an adjusted F-score calculation.
Results F1 score for each optimized model ranged from 0.951 (“Fall”) to 0.544 (“Environment”). The bag-of-words approach proved optimal for 12 of 14 labels, and the naïve Bayes algorithm performed best for nine labels. Linear support vector machine was demonstrated as optimal for three labels and XGBoost for four of the 14 labels. Labels with more distinctly associated terms performed better than less distinct themes, as shown by a Pearson's correlation coefficient of 0.634.
Conclusions We were able to demonstrate an analytical pipeline that broadly applies NLP and predictive modeling to categorize patient safety reports from multiple facilities. This pipeline allows analysts to more rapidly identify and structure information contained in patient safety data, which can enhance the evaluation and the use of this information over time.
Calcification Propensity in Serum and Cardiovascular Outcome in Peripheral Artery Disease
Authors: Bojic Marija , Bielesz Bernhard , Cejka Daniel , Schernthaner Gerit-Holger , Höbaus Clemens 
Published: October 2021

AbstractPeripheral artery disease (PAD) has been shown to be linked to elevated cardiovascular risk. The novel T50 test quantifies calcification propensity of serum and has been associated with cardiovascular events and mortality in patients with chronic kidney disease (CKD) and in the general population. This study investigated the association of calcification propensity measured by the T50 test in 287 patients with PAD without severe CKD. Major cardiovascular events (MACEs) including nonfatal stroke and nonfatal myocardial infarction and all-cause death (MACE + ) were evaluated after a median follow-up of 4 years and long-term cardiovascular and all-cause mortality after a median follow-up of 8.7 years by Kaplan–Meier and Cox regression analyses. Mean T50 time was 268 ± 63 minutes in the study cohort (age 69 ± 10 years, 32% women, 47% diabetes). Low T50 values that signify high calcification propensity were significantly associated with the occurrence of MACE+ (hazard ratio [HR]: 0.72; 95% confidence interval [CI]: 0.55–0.94). This association sustained multivariate adjustment for cardiovascular risk factors (CVRFs), Fontaine PAD stage, and prevalent media sclerosis (HR: 0.65; CI: 0.47–0.91). Cardiovascular mortality was significantly associated with T50 after multivariate adjustment for CVRF (HR: 0.72; CI 0.53–0.99), but not all-cause mortality (HR: 0.80; CI: 0.64–1.01). In conclusion, calcification propensity associates with MACE+ and cardiovascular mortality in patients with PAD.
Autoimmune Hemolytic Anemia Due to Spondyloenchondrodysplasia with Spastic Paraparesis and Intracranial Calcification due to Mutation in ACP5
Authors: Aylan Gelen Sema , Kara Bülent , Eser Şimsek Isil , Güngör Mesut , Zengin Emine , Sarper Nazan 
Published: November 2021

AbstractSpondyloenchondrodysplasia (SPENCD) is a rare spondylometaphyseal skeletal dysplasia with characteristic lesions mimicking enchondromatosis and resulting in short stature. A large spectrum of immunologic abnormalities may be seen in SPENCD, including immune deficiencies and autoimmune disorders. SPENCD is caused by loss of tartrate-resistant acid phosphatase activity, due to homozygous mutations in ACP5, playing a role in nonnucleic-acid-related stimulation/regulation of the type I interferon pathway. In this article, we presented a 19-year-old boy with SPENCD, presenting with recurrent autoimmune hemolytic anemia episodes since he was 5 years old. He had short stature, platyspondyly, metaphyseal changes, intracranial calcification, spastic paraparesis, and mild intellectual disability. He also had recurrent pneumonia attacks. The clinical diagnosis of SPENCD was confirmed by sequencing of the ACP5 gene, and a homozygous c.155A > C (p.K52T) variation was found, which was reported before as pathogenic. In conclusion, in early onset chronic autoimmune cytopenias an immune dysregulation may often have a role in the etiology. Associating findings and immunologic functions should be carefully evaluated in such patients in the light of the literature. The present case shows the importance of multisystemic evaluation for the detection of SPENCD that has a monogenic etiology.
Wound Complications with Multiple Wrist Incisions in Distal Radius Reconstruction
Authors: Meaike Joshua J. , Athens Christian , Sgromolo Nicole , Shin Alexander Y. , Rhee Peter C. 
Published: October 2021

Abstract
Background Placement and spacing of skin incisions are important for maintaining soft tissue perfusion and viability, particularly in the setting of local trauma.
Question/Purpose The aim of this article is to determine if multiple skin incisions in the surgical management of distal radius fractures result in an increased risk of postoperative wound complications, particularly in the setting of high-energy mechanisms of injury with substantial initial displacement and associated soft-tissue insult that require multiple incisions for distal radius reconstruction.
Patients and Methods A multicenter, retrospective chart review was performed for all adult patients who underwent open reduction, internal fixation of a closed distal radius fracture with multiple (≥2) hand, and wrist incisions with minimum follow-up of 6 weeks. Primary outcome measures included wound healing complications such as partial or complete skin necrosis, dehiscence, delayed healing, and superficial or deep infections.
Results For 118 wrists, a total of 305 incisions were utilized, averaging 2.6 incisions per patient (range: 2–6) with the flexor carpi radialis and dorsal distal radius approaches occurring in 86 and 78% of cases, respectively. One patient was identified as having a pyogenic granuloma along an incision. However, two patients were identified as having wound concerns, including fracture blisters (n = 1) and wound margin epidermolysis (n = 1) along the incision. There were no cases of skin bridge necrosis, delayed healing, wound dehiscence, or infection.
Conclusions There is no increased risk of wound healing complications with the use of multiple skin incisions (≥2) in the surgical management of distal radius fractures, afforded by the abundant and robust angiosomes around the wrist. Surgeons should have the confidence to utilize the necessary number of skin incisions to effectively reconstruct distal radius fractures.
Fertilization potential of cold-stored Fowler’s toad (
Authors: Arregui Lucia , Kouba Andy J. , Germano Jennifer M. , Barrios Laura , Moore Marian , Kouba Carrie K. 
Published: October 2021

Asynchrony of gamete release is problematic in amphibian captive breeding programs but can be overcome by short-term storage of spermatozoa. Hormonally induced sperm from the model species Anaxyrus fowleri were used to determine storage conditions for optimal fertilisation capacity. Sperm motility was measured over time, as a function of storage temperature (4°C or 22°C) and solution osmolality (7–40 mOsm/kg). Sperm at 40 mOsm/kg (spermic urine) stored at 4°C exhibited higher motility compared to 22°C. Also, sperm stored at 40 mOsm/kg retained higher motility compared to sperm stored below 15 mOsm/kg at both temperatures. Under optimal storage conditions (40 mOsm and 4°C) a 30% decrease in sperm motility occurred within 24 h, however, subsequent loss of sperm motility was lower (<10%/day) for days 2–8 thereafter. Sperm samples stored for 1–8 days under optimal conditions were tested for fertilising capacity by conducting in vitro fertilisation trials. Sperm stored for 8 days yielded 48% neurula development, similar to sperm stored for 1 day, which produced 60% neurula development. Overall, sperm stored for up to 8 days at 4°C as spermic urine retained fertilising capacity and thus can be used to circumvent asynchronous gamete release in assisted breeding efforts for amphibians.
Pregnancy prevention and unintended pregnancy across gender identity: a cross-sectional study of college students
Authors: Reynolds Colleen A. , Charlton Brittany M. 
Published: November 2021

Using data from the Fall 2015 through Spring 2018 National College Health Assessment, we examined receipt of pregnancy prevention information and unintended pregnancy by gender identity among participants aged 18–25 years who were assigned female at birth (n = 185 658). Non-binary students were more likely than cisgender students to report wanting (adjusted risk ratio [ARR]: 1.12; 95% CI: 1.08–1.16), receiving (ARR: 1.09; 95% CI: 1.04–1.13), and having an unmet need for (ARR: 1.10; 95% CI: 1.02–1.19) pregnancy prevention information from their school. Transmasculine students did not significantly differ from cisgender students for these outcomes. Non-binary and transmasculine students were as likely as cisgender students to have a past-year unintended pregnancy. Non-binary and transmasculine young people are at risk for unintended pregnancy and need access to comprehensive sexual education, reproductive health counseling, and care.
Performance behavior of pocketed journal bearing employing non-newtonian lubricant
Authors: Bhardwaj Vivek 
Published: November 2021

Abstract
This paper reports the improvement in the performance behaviors of non-Newtonian fluid lubricated journal bearings employing a rectangular pocket. Coefficient of friction and load carrying capacity of pocketed and non-pocketed bearings are reported and discussed. In the numerical approach, non-Newtonian behavior of lubricant is simulated employing the power law model. Various cases of bearings having different axial length to diameter ratios [(L/D) in the range 0.5–2.0] are considered. Lubricants of different rheology viz. pseudoplastic, Newtonian and shear thickening were employed for lubricating the bearings. Rheology of lubricant is controlled by varying the power law index n in the range 0.9–1.1. Based on the investigation, it is found that the pocketed bearing yields significantly reduced coefficient of friction and higher load carrying capacity in comparison to the non-pocketed bearing. Also, the performance of pocketed bearing improves with the increase in the power law index. Best performance of the pocketed bearing is observed for the bearing having L/D value of 0.5 at high eccentricity ratio when lubricated with the shear thickening fluid (n=1.1).
Mapping cell-to-cell mitochondria transfer in obesity using high-dimensional spectral flow cytometry
Authors: Borcherding Nicholas , Moley John , Field Rachael , Brestoff Jonathan R 
Published: October 2021

Abstract
Obesity is a metabolic disease that promotes the development of a number of other pathologies. Despite its high disease burden, the underlying pathophysiology of obesity is poorly understood. Emerging research has indicated that adipocytes transfer their mitochondria to macrophages in white adipose tissue as a mechanism of cell-to-cell communication and that this process is impaired in obesity. However, the diversity of intercellular mitochondria transfer axes that occurs in adipose and its regulation in obesity are not known. Here, we utilized 31-color spectral flow cytometry of adipocyte-specific mitochondria reporter (MitoFat) mice to comprehensively analyze intercellular mitochondria transfer from adipocytes to other cell types in white, beige, and brown adipose tissues. Employing manifold machine learning, we generated reference clusters of cells in 5-month (young) and 20-month-old (aged) MitoFat mice fed a normal chow diet (low fat diet). Using the reference clusters and manifold, we then mapped differences in immune cell populations using nearest neighbor search approximations in MitoFat mice fed normal chow, high-fat diet (HFD), high-fat diet with low palmitate (LP-HFD). The degree of mitochondria transfer from adipocytes to each of the various cell clusters was determined for each tissue and for each condition. We observed that adipocytes transfer their mitochondria to a wide range of immune cell populations, most notably macrophages. Although aged mice develop obesity, surprisingly they do not exhibit decreased mitochondria transfer from adipocytes to macrophages in vivo in white, beige, or brown adipose tissue. In contrast, young mice fed a HFD highly enriched in palmitate exhibit obesity and markedly reduced mitochondria transfer from adipocytes to macrophages. The decrease in mitochondria transfer was largely ameliorated by the replacement of palmitate with medium chain fatty acids, suggesting a potential direct dietary mechanism in the alteration of mitochondria transfer. Overall, the 31-color quantification increased granularity, allowing us to quantify differences in immune populations and mitochondria transfer by tissue, age, and diet. Similar machine-learning approaches could be used to investigate both basic biological and clinical questions by effectively reducing dimensions, mitigating batch effect, and enabling comparisons across different tissues, timepoints, or conditions.
Development and Validation of a Liquid Chromatography Mass Spectrometry Method for Simultaneous Measurement of 25-OH D3, epi-25-OH D3, 25-OH D2, Vitamin A, α-Tocopherol, and γ-Tocopherol
Authors: Xiao Yi , Leung Edward 
Published: October 2021

Abstract

Background
Circulatory fat-soluble vitamin levels are commonly measured to identify deficiencies that may lead to rickets, osteomalacia, night blindness, and reversible motor and sensory neuropathies. We developed and validated a rapid and robust LC-MS/MS method that simultaneously measures 25-OH D3, epi-25-OH D3, 25-OH D2, vitamin A, α-tocopherol, and γ-tocopherol for clinical use.

Method
100 µL of serum was mixed with isotope-labeled internal standard and extracted using a 96-well supported-liquid extraction plate with 1.5 mL of hexanes/isopropanol (90/10) (v/v). Dried eluate was reconstituted with 100 µL of methanol/water (90/10) (v/v) and analyzed by LC-MS/MS with a 10-minute gradient. Accuracy was assessed using NIST Standard Reference Materials SRM972a and SRM968f, patient comparison analysis with a LC-MS/MS method at a reference lab, and spike-recovery studies using patient sera and vitamin D-depleted serum. Analytical measurement range (AMR) was determined by spiking 6 analytes into vitamin D-depleted serum to give 7 specimens at varying concentrations. The lower limit of the measuring interval (LLMI) was assessed using 6 pooled specimens with varying low concentrations of each analyte over 20 days. Precision (repeatability and reproducibility) was assessed using quality control materials. Interference studies were performed using pooled patient specimens spiked with varying concentrations of hemoglobin, bilirubin, or intralipid. Matrix effect was assessed by post-column infusion and by matrix dilution with saline.

Results
The method was linear covering physiological concentrations with r2 &gt; 0.99. Repeatability and reproducibility were &lt;15% CV at all QC levels. LLMI for 25-OH D3, epi-25-OH D3, 25-OH D2, vitamin A, α-tocopherol, and γ-tocopherol were 4 ng/mL (15% CV), 4 ng/mL (15% CV), 4 ng/mL (18% CV), 1 µg/dL (20% CV), 0.2 µg/mL (20% CV), and 0.2 µg/mL (8% CV). Recoveries for NIST Standard Reference Materials were between 92 - 111% and between 81 - 122% for spike-recovery studies. Passing-Bablok analyses for vitamin D total, vitamin A, and α-tocopherol demonstrated slopes between 1.04 and 1.11 and r2 between 0.94 and 0.96. Minimal matrix effect was observed.

Conclusions
We have developed and validated a comprehensive and rapid LC-MS/MS method for the simultaneous measurement of 25-OH D3, epi-25-OH D3, 25-OH D2, vitamin A, α-tocopherol, and γ-tocopherol for clinical use.