bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multitargeting Strategy Using Tetrathiomolybdate and Lenvatinib: Maximizing Anti-Angiogenesis Activity in a Preclinical Liver Cancer Model
Authors: Nan LI , Yuan WAN , Jianyong YANG , Yonghui Huang 
Published: December 2021

Abstract
Purpose
To investigate the suppressing tumor-promoting effects via multi-anti-angiogenesis activity of the copper chelator (Ammonium Tetrathiomolybdate, TM) combined with lenvatinib for hepatocellular carcinoma.
Material and methods
Fifty-five C57 mice were injected subcutaneously with Hepa1-6 hepatoma cell suspensions into the right posterior thigh. Seven days later, all subcutaneous tumors were formed and the mice were randomly distributed into 5 groups: TM Group (G1), Lenvatinib Group (G2), TM+Lenvatinib Group (G3), Control Group (G4), and Copper (II) Gluconate Group (G5). And copper concentrations in serum and tumors were measured at the predetermined times. After fourteen days of treatments, tumor weight and volumes were analyzed, histology was observed, and the expressions of VEGF and microvessel density (MVD) in tumor tissues were measured by immunohistochemistry (IHC).
Results
Average concentration of copper in serum was 405.14 ug/L, 480.44 ug/L, and 679.80 ug/L in normal mice, in mice on 7 days after implantation, and in the control group, respectively. Similarly, intratumoral copper concentrations were greater in G4 mice (1511.90 ug/L) than mice on 7 days after implantation (852.80 ug/L) (p < 0.05). And the serum concentration of copper was 363.65 ug/L, 508.83 ug/L, 370.52 ug/L, 822.12 ug/L in G1, G2, G3, and G5 [G5 vs other Groups, all p < 0.05; (G1, G2, and G3) vs G4, p < 0.05; G1 vs G2, p = 0.013; G2 vs G3, p = 0.018; G1 vs G3, p = 0.903] while intratumoral copper concentrations was 674.31 ug/L, 988.91 ug/L, 550.52 ug/L, and 3004.95 ug/L in G1, G2, G3, and G5 And the average tumor weight was 0.55 g, 0.44 g, 0.08 g, 1.37 g, 3.11 g in the mice of G1, G2, G3, G4, and G5, respectively. [G5 vs other Groups, all p < 0.05; (G1, G2, and G3) vs G4, p < 0.05; G1 vs G3, p < 0.05; G2 vs G3, p < 0.05; G1 vs G2, p > 0.05]. Furthermore, tumors were collected for HE staining and IHC examination. The expression levels of VEGF in G1, G2, and G3 were 43.75, 32.48, and 15, and all of them were significantly lower than those in G4 (64.28) and G5 (89.03) (G4 vs G5, p < 0.05). Similarly, the trend of MVD was just like that of VEGF in the five groups whereas no significant difference occurred in G1 and G2.
Conclusion
The study shows that there is a significant positive correlation between tumor load and copper. Administration of copper can promote tumor progression, and copper chelating could suppress tumor growth. The combination of TM with lenvatinib can reduce tumor angiogenesis and improved the effect of antitumor treatment. These findings offer basic data support and theoretical foundation for the clinical application of the combination therapy.
Contraceptive Use in Women with Inherited Metabolic Disorders: A Retrospective Study and Literature Review
Authors: Gold Jessica , Gold Nina B , DeLeon Diva D , Ganetzky Rebecca 
Published: December 2021

Abstract
Background
Reproductive planning is an emerging concern for women with inherited metabolic disease (IMD). Anticipatory guidance on contraception is necessary to prevent unintended pregnancies in this population. Few resources exist to aid informed decision-making on contraceptive choice. A retrospective case-control study was performed to examine trends in reproductive planning for adolescent and adult women seen at the Children’s Hospital of Philadelphia (CHOP). Literature review on contraception and IMD was performed to assess global use.
Results
In a cohort of 221 reproductive-aged female IMD patients, 29.4% reported routine contraceptive use. Anticipatory guidance on contraception was provided by metabolism doctors to 36.8% of patients during the study period. Contraception discussion was more likely to occur in women older than 21 years, who lived independently and were followed by gynecology. Women who received contraception counseling from their metabolism provider were 40-fold more likely to use regular contraception. Use of combined hormonal contraceptives was most commonly reported, but contraception choice varied by age and IMD.
Conclusion
Metabolism providers are ideally suited to provide guidance on contraception to women with IMD. Reproductive planning should be addressed routinely using shared decision-making. Contraceptives should be selected for their efficacy, effects on metabolism, and likelihood of patient adherence.
The Normal and Fibrotic Mouse Lung in Situ Classified by High Dimensional Single Cell Analysis on Routinely Processed Tissue
Authors: Ciccimarra Roberta , Bolognesi Maddalena M. , Zoboli Matteo , Cattoretti Giorgio , Stellari Fabio F. , Ravanetti Francesca 
Published: December 2021

Abstract
Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process.
Metabolomic Spectra For Phenotypic Prediction of Malting Quality In Spring Barley
Authors: Guo Xiangyu , Jahoor Ahmed , Jensen Just , Sarup Pernille 
Published: December 2021

Abstract
The objectives were to investigate prediction of malting quality (MQ) phenotypes in different locations using information from metabolomic spectra, and compare the prediction ability using different models and different sizes of training population (TP). A total of 2,667 plots of 564 malting spring barley lines from three years and two locations were included. Five MQ traits were measured in wort produced from each individual plot. Metabolomic features (MFs) used were 24,018 NMR intensities measured on each wort sample. Models involved in the statistical analyses were a metabolomic best linear unbiased prediction (MBLUP) model and a partial least squares regression (PLSR) model. Predictive ability within location and across locations were compared using cross-validation methods. The proportion of variance in MQ traits that could be explained by effects of MFs was above 0.9 for all traits. The prediction accuracy increased with increasing TP size but when the TP size reached 1,000, the rate of increase was negligible. The number of components considered in the PLSR models can affect the performance of PLSR models and 20 components were optimal. The accuracy of individual plots and line means using leave-one-line-out cross-validation ranged from 0.722 to 0.865 and using leave-one-location-out cross-validation ranged from 0.517 to 0.817.In conclusion, it is possible to carry out metabolomic prediction of MQ traits using MFs, the prediction accuracy is high and MBLUP is better than PLSR if the training population is larger than 100. The results have significant implications for practical barley breeding for malting quality.
Does Parental Marital Status at Birth Influence the Risk of Children and Grandchildren Requiring Local Authority Care? A Retrospective Study of the Scottish Birth Cohort
Authors: Colvin Michael 
Published: December 2021

Abstract
Background

- Demand in children’s social care remains at historically high levels across the UK. The number of children in local authority care in Scotland increased every year between 1998 and 2013. This study explores a possible trans-generational influence of historical changes in family structure at birth.
Method

- Children who were accommodated by a typical Scottish local authority from 2000 to 2013 were identified. The parental marital status on birth registrations of the children and their parents was compared to all Scottish registrations in national birth cohorts and relative risk (RR) was calculated for different birth circumstances.
Results

- The children who entered care were more likely to have been born to unmarried parents than their peers (RR 7.8, CI 6.3-9.6). Their mothers (RR 3.2, CI 2.7-3.9) and fathers (RR 1.5, CI 1.1-2) were also more likely than their peers to have been born to unmarried parents. The accumulated risk of young children entering care if their parents and maternal grandparents had been unmarried on birth registrations was very large (RR 23.3, CI 12.5-43.5).
Conclusion

- Parental marriage is protective against entering local authority care for children in Scotland. Historical changes in family structure at birth may influence demand in children’s services.
Lipoxin A4 Regulates M1/M2 Macrophage Polarization via FPR2-IRF Pathway
Authors: Yuan Jixiang , Lin Feihong , Chen Lichen , Chen Weikang , Pan Xiaodong , Bai Yongheng , Cai Yong , Lu Hong 
Published: December 2021

Abstract
Lipoxin A4 (LXA4) has been shown to have anti-inflammatory activity, but its underlying molecular mechanisms are not clear. Herein, our team investigated the potential role of LXA4 in the macrophage polarisation and elucidated its possible molecular mechanism. The RAW264.7 macrophage cell line was subjected to pre-treatment with LXA4 with or without lipopolysaccharides (LPS) and interleukin-4 (IL-4). In cultured macrophages, LXA4 inhibits LPS-induced inflammatory polarization, thereby decreasing the release of proinflammation cell factors (IL-1β, IL-6, TNF-α) and increasing the release of antiinflammation cytokines (IL-4 and IL-10). Notably, the inhibitory effect of LXA4 on inflammation macrophage polarisation was related to the downregulation of p-NF-κB p65 and IRF5 activity, thereby downregulating LPS-induced phenotypic and functional polarization of macrophage M1 via the FPR2/IRF5 signaling pathway. Moreover, LXA4 also promotes the IL-4-induced polarization of M2 macrophages by promoting the FPR2/IRF4 signaling pathway. Therefore, Lipoxin A4 regulates M1/M2 polarization of macrophages via FPR2-IRF pathway.
SeisDeNet: An Intelligent Seismic Data Denoising Network for the Internet of Things
Authors: Sang Yu , Peng Yanfei , Lu Mingde , Li Liquan 
Published: December 2021

Abstract
Deep learning (DL) has attracted tremendous interest in various fields in last few years. Convolutional neural networks (CNNs) based DL architectures have been successfully applied in computer vision, medical image processing, remote sensing, and many other fields. A recent work has proved that CNNs based models can also be used to handle geophysical problems. Due to noises in seismic signals acquired by geophone equipment this kind of important multimedia resources cannot be effectively utilized in practice. To this end, from the perspective of seismic exploration informatization, this paper takes informatization data in seismic signal acquisition and energy exploration field using cutting-edge technologies such as Internet of things and cloud computing as the research object, presenting a novel CNNs based seismic data denoising (SeisDeNet) architecture is suggested. Firstly, a multi-scale residual dense (MSRD) block is built to leverage the characteristics of seismic data. Then, a deep MSRD network (MSRDN) is proposed to restore the noisy seismic data in a coarse-to-fine manner by using cascading MSRDs. Additionally, the denoising problem is formulated into predicting transform-domain coefficients, by which noises can be further removed by MSRDNs while richer structure details are preserved comparing with the results in spatial domain. By using synthetic seismic records, public SEG and EAGE salt and overthrust seismic model and real field seismic data, the proposed method is qualitatively and quantitatively compared with other leading edge schemes to evaluate it performance, and some results shows that the proposed scheme can produce data with higher quality evaluation while maintaining far more useful data comparing with other schemes. The feasibility of this approach is confirmed by the denoising results, and this approach is shown to be promising in suppressing the seismic noise automatically.
The anti-ovarian carcinoma activity of L-amino Acid Oxidase from Crotalus adamanteus venom in vivo and in vitro
Authors: Bo Li , Xiong Yan , He Qiyi , Yu Xiaodong , Li Bo , Xiang Bolin , Wang Ping , Chen Xiang , Song Ziwei 
Published: December 2021

Abstract
The anti-tumor potential of animal toxins has fully attracted the attention of researchers. Snake venoms is a complex mixture of different components and has revealed high toxicity on normal and tumoral tissues or cells. The snake venom L-Amino-acid oxidase (svLAAO) has grown up to be a critical research target in molecular biology sciences and medicine sciences since widespread presence and various biological roles, including antitumor application. We found that Crotalus adamanteus (C. adamanteus) venom LAAO significantly decreased the viability of ovarian cancer cells and caused morphological changes preceded cell death. Cell experiments confirmed that C. adamanteus venom LAAO caused alterations of intrinsic or extrinsic apoptosis pathway-related genes in ovarian cancer cells. Animal experiments and histological analysis also proved that C. adamanteus venom LAAO could effectively inhibit the damage of ovarian cancer to tissues. The major apoptosis induction of C. adamanteus venom LAAO on ovarian cancer cells can be blocked by catalase, suggesting that the cytotoxicity of C. adamanteus venom LAAO on ovarian cancer cells was mainly mediated by H2O2. Our preliminary results revealed that C. adamanteus venom LAAO may induce apoptosis of ovarian cancer cells through the death receptor pathway and mitochondrial pathway. It is inferred that C. adamanteus venom LAAO will be some advantages in New Drug Research and Development of antitumor drugs in the future. Nevertheless, extra studies on the pharmacological actions and molecular mechanism of svLAAO in anti-cancer are necessary in order to better promote its application.
WITHDRAWN: Researching Social Anxiety in an Online Sample of Patients
Authors: Khalifa Samar 
Published: December 2021

Abstract
Objective
The research paper is discussing and investigating the disorder of social anxiety in terms of psychology and mental health.
Methods
A Psycom Social Anxiety Test was conducted on a sample of online patients (n = 41) with social anxiety to use this data in further research for social anxiety disorder.
Results
The data showed a moderate correlation between the question “Are you extremely conscious of your actions when in social settings because you fear they might offend someone or you could be rejected?” and the question “Are your work life, home life, social life, and/or relationships affected by your anxiety?”.
Conclusion
There is a relation between the consciousness of the actions and the social life so social anxiety arises in those objects who suffer from social fear.
Is iron status associated with markers of non-communicable disease in Indian children?
Authors: Ghosh Santu , Thomas Tinku , Kurpad Anura , Sachdev Harshpal Singh 
Published: December 2021

Abstract
BackgroundHigh body iron stores have been associated with risk for non-communicable diseases (NCD) like diabetes (high fasting blood sugar, FBS), hypertension (HTN) or dyslipidaemia (high total cholesterol, TC) in adults, but not in adolescent children. This is relevant to iron supplementation and food iron fortification programs that are directed at Indian children. MethodsThe association of NCD with serum ferritin (SF) was examined using logistic additive models, adjusted for confounders such as age, Body Mass Index, C-Reactive Protein, haemoglobin and sex, in adolescent (10-19 years old) participants of the Indian Comprehensive National Nutrition Survey. The interaction of these associations with wealth and co-existing prediabetes was also examined. A scenario analysis was also done to understand the impact of iron fortification of cereals on the prevalence NCD among adolescents.ResultsThe odds ratio (OR) of high FBS, HTN and TC were 1.05 (95% CI 1.01-1.08), 1.02 (95% CI: 1.001-1.03) and 1.04 (95% CI: 1.01-1.06) respectively for every 10µg/L increase in SF. The odds for high TC increased with co-existing prediabetes. The scenario analysis showed that providing 10 mg of iron/day by fortification could increase the prevalence of high FBS by 2%-14% across states of India. Similar increments in HTN and TC can also be expected.ConclusionsHigh SF is associated with a significant risk for NCD in adolescents, and this is dependent on the wealth class, and on co-existing prediabetes. This should be considered when evaluating the benefits and harms of enhancing iron intake in anaemia prevention programs.
Full-range birefringence control with piezoelectric MEMS-based metasurfaces
Authors: Meng Chao , Thrane Paul , Ding Fei , Bozhevolnyi Sergey 
Published: December 2021

Abstract
Dynamic polarization control is crucial for emerging highly integrated photonic systems with diverse metasurfaces being explored for its realization1–6, but efficient, fast, and broadband operation remains a cumbersome challenge. While efficient optical metasurfaces (OMSs) involving liquid crystals suffer from inherently slow responses1, other OMS realizations are limited either in the operating wavelength range (due to resonances involved)2,3 or in the range of birefringence tuning4–6. Capitalizing on our development of piezoelectric micro-electro-mechanical system (MEMS) based dynamic OMSs7, we demonstrate reflective MEMS-OMS dynamic wave plates (DWPs) with high polarization conversion efficiencies (~ 75%), broadband operation (~ 100 nm near the operating wavelength of 800 nm), fast responses (< 0.4 milliseconds) and full-range birefringence control that enables completely encircling the Poincaré sphere along trajectories determined by the incident light polarization and DWP orientation. Demonstrated complete electrical control over light polarization opens new avenues in further integration and miniaturization of optical networks and systems8,9.
Correlation Between Ultrawide-Field Fluorescence Contrast Results and White Blood Cell Indexes in Diabetic Retinopathy
Authors: Huang Lili , Li Lele , Wang Min , Zhang Dongmei , Song Yu 
Published: December 2021

Abstract
Background: Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes. DR involves a state of systemic inflammation, and chronic inflammation can promote microvascular and macrovascular diseases in diabetic patients and accelerate disease progression. Ultrawide-field FFA (UWFA) systems are increasingly being used to examine a wider retina. To explore the correlation between the different manifestations of retinopathy under UWFA and the systemic indicators of white blood cells in patients with diabetic retinopathy .Methods: This retrospective study included the hospitalized DR patients in the Department of Ophthalmology and Endocrinology of the Affiliated Hospital 2 of Nantong University between January 2016 and March 2019. This study examined the correlations between the UWFA examination results and glycated hemoglobin (HbA1c), routine blood tests, blood coagulation function, liver and kidney function, and the neutrophil-to-lymphocyte ratio of patients with clinically diagnosed DR during hospitalization.Results: A total of 115 patients with DR (53 females and 62 males) were included (199 eyes: 102 right eyes and 97 left eyes). UWFA revealed that most eyes (77.4%) had grade 4 microvascular leakage, 52.8% had grade 0 capillary non-perfusion area, 59.3% had grade 0 neovascularization, and 92.0% had grade 0 fibrous proliferative membranes. Microvascular leakage was correlated with the NLR (r=0.186, P=0.027). Capillary non-perfusion area was correlated with the monocyte ratio (r=0.144, P=0.042) and the eosinophil ratio (r=0.123, P=0.044). Neovascularization was correlated to the monocyte ratio (r=0.324, P=0.018). Finally, the fibrous proliferative membrane was correlated to the monocyte ratio (r=0.418, P=0.002). Only the eosinophil ratio was independently associated with proliferative DR (odds ratio=1.25, 95% confidence interval: 1.04-1.51, P=0.018).Conclusion: The results of UWFA imaging in patients with DR are correlated with white blood cell population indexes. The eosinophil ratio was independently associated with proliferative DR.
Response Surface Mesh with the Outer Input Method
Authors: Duarte Francisco Daniel Filip 
Published: December 2021

Abstract
Artificial intelligence in general and optimization tasks applied to the design of very efficient structures rely on response surfaces to forecast the output of functions, and are vital part of these methodologies. Yet they have important limitations, since greater precisions require greater data sets, thus, training or updating larger response surfaces become computationally expensive or unfeasible. This has been an important bottle neck limitation to achieve more promising results, rendering many optimization and AI tasks with a low performance.To solve this challenge, a new methodology created to segment response surfaces is hereby presented. Differently than other similar methodologies, this algorithm named outer input method has a very simple and robust operation, generating a mesh of near isopopulated partitions of inputs which share similitude. The great advantage it offers is that it can be applied to any data set with any type of distribution, such as random, Cartesian, or clustered, for domains with any number of coordinates, significantly simplifying any metamodel with a mesh ensemble.This study demonstrates how one of the most known and precise metamodel denominated Kriging, yet with expensive computation costs, can be significantly simplified with a response surface mesh, increasing training speed up to 567 times, while using a quad-core parallel processing. Since individual mesh elements can be parallelized or updated individually, its faster operational speed has its speed increased.
Impact of the Gamma Variant on the ICU Admission in a Hospital of the Autonomous City of Buenos Aires (CABA), Argentina
Authors: Mandich Veronica , Chiacchiara Daniel , Bravo Marcelo , Pilipec Antonio , Grimaldi Santiago , Cotes Johnis , Paniouk Victor , Arevalo Henry , Escobar Oswaldo , Flach Sebastian Perez , Pozo Amilcar Herbas , Aimar Alejandro , Vargas Mario , Sañudo Karina 
Published: December 2021

Abstract
Purpose: Comparison between patients admitted to the Intensive Care Unit (ICU) with ventilatory support during the first and second wave (Gamma variant) of COVID 19 in a tertiary hospital in the City of Buenos Aires.Method: Descriptive, observational, prospective cohort study in ICU of an acute general hospital in Argentina, with patients adult patients COVID-19 admitted consecutively to the ICU. The ICU the main variables of interest: Demographic and laboratory data, history, duration of MV, mortality.Results: 44 patients were recruited during the first wave, and 80 in the second. Significant differences were observed in the second wave for age (60, [SD 11]; vs 68 [SD 11]; p <0.01), days with symptoms (9 [SD 5]; vs 4.5 [SD 3]; p <0.01), duration of MV (10 days [SD 7]; vs 16 [SD 13]; p <0.01), Ferritin (1450 mcg / l [SD 547]; vs 941 mcg / l [ SD 593]; p 0.04) and use of antibiotics (97% vs 45% p <0.01). There was no difference in mortality (66% vs 78% p 0.14) but there was in its primary causes; being more frequent hypoxemia associated with sepsis in the second wave (40% vs 3.4% p <0.01) and hypoxemia and multiorgan failure in the first (28% vs 4% p <0.01).Conclusions: During the second wave of the pandemic, due to Gamma variant, more patients were admitted to our unit, younger, with a longer evolution time, a shorter time on MV and a greater inflammatory response, with no difference in mortality, although there was a difference in its causes.