bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global change factors influence different aspects of arbuscular mycorrhizal fungal communities
Authors: Zheng Junqiang , Cui Mingming , Wang Cong , Wang Jian , Wang Shilin , Sun Zhongjie , Ren Feirong , Wan Shiqiang , Han Shijie 
Published: February 2021

Abstract
BackgroundThe functional diversity of arbuscular mycorrhizal fungi ( AMF) affects the resistance and resilience of plant communities to environmental stresses. However, considerable uncertainty remains about how the complex interactions among elevated atmospheric CO 2 (eCO 2 ), nitrogen deposition (eN), increased precipitation (eP), and warming (eT) affect AMF communities. These global change factors (GCFs) always occur simultaneously, and their interactions likely affect AMF community structure and assembly processes. In this study, the interactive effects of these four GCFs on AMF communities were explored in an open-top chamber field experiment in a semiarid grassland. ResultsElevated CO 2 , eN, eT, and eP and their interactions did not affect AM fungal biomass. The relative abundance of Paraglomus increased with N addition across treatment combinations, whereas that of Glomus decreased with N addition, especially combined with eT and eCO 2 . Precipitation, T, and N affected AMF phylogenetic α-diversity, and the three-way interaction among CO 2 , T, and N affected taxonomic and phylogenetic α-diversity. Nitrogen addition significantly affected the β-diversity of AMF communities. Both variable selection and dispersal limitation played major roles in shaping AMF communities, whereas homogeneous selection and homogenizing dispersal had almost no influence on AMF community assembly. The contribution of variable selection decreased under eCO 2 , eN and eT, but not under eP. The contribution of dispersal limitation decreased under eCO 2 , eT, and eP but it increased under eN. The assembly of AMF communities under the sixteen GCF combinations was strongly influenced by dispersal limitation, variable selection and ecological drift. ConclusionsElevated CO 2 , warming, N addition, and increased precipitation influenced different aspects of AMF communities. The interactive effects of the four GCFs on AMF communities were limited. Collectively, the results of this study suggest that AMF communities in semiarid grasslands can resist changes in the global climate.
Risk factors to predict ocular metastasis in older adult patients with gastric cancer:LDL, ApoA1, and CA724
Authors: Shi Wenqing , Wu Shinan , Sun Tie , Shu Huiye , Yang Qichen , Li Qiuyu , Su Ting , Pan Yicong , Liang Rongbin , Shao Yi 
Published: February 2021

Abstract
Background: Gastric cancer (GC) is one of the most common malignancies in the population. Although the incidence of GC has reduced, patient prognosis remains poor. Ocular metastases (OM) from GC are rare, and the occurrence of OM is often indicative of severe disease. The purpose of this study was to explore the risk factors for OM of GC.Methods: A total of 1165 older adult patients with GC were enrolled in this study from June 2003 to May 2019 and divided into OM and non-ocular metastasis (NOM) groups. Chi-square and independent samples t tests were used to determine whether differences in demographic characteristics and serological indicators (SI) between the two groups were significant. In addition, binary logistic regression was used to analyze the value of various SI as risk factors for OM in patients with GC. The statistical threshold was set as P < 0.05. Finally, receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic value of various SI in differentiating the occurrence of OM in patients with GC. Results: The incidence of OM in older adults with GC was 1.1%. Adenocarcinoma was the most common type of GC in both groups, and there was no significant difference in demographic characteristics, including sex and age between the groups. Low-density lipoprotein (LDL), carbohydrate antigen-724 (CA724), and carcinoembryonic antigen levels were significantly higher in the OM group than the NOM group, while those of apolipoprotein A1 (ApoA1) were significantly lower in the OM than the NOM group. Binary logistic analysis showed that LDL, ApoA1, and CA724 were independent risk factors for OM in patients with GC (P < 0.001,P = 0.033, and P = 0.008, respectively). ROC curve analysis generated area under the curve (AUC) values of 0.881, 0.576, and 0.906 for LDL, ApoA1, and CA724, respectively. In addition, combined analysis of LDL, ApoA1, and CA724 generated the highest AUC value of 0.924 (P < 0.001).Conclusion: Among SI, LDL, ApoA1, and CA724 have predictive value for the occurrence of OM in GC, with the three factors combined having the highest value.
Transcriptome Changes in Porcine Granulosa Cells in Response to Gossypol Cytotoxicity
Authors: Hong Min-Wook , Choi So-Young , Kim Hun , Lee Sung-Jin 
Published: February 2021

Abstract
Background: Gossypol (GP) is a polyphenolic compound in cottonseed. In porcine, GP affects female reproduction and the respiratory system. The aim of this study was to identify differentially expressed genes in porcine granulosa cells (GCs) treated with GP (6.25 and 12.5 μM) for 72 h, in vitro . Results: In GP-treated groups (6.25 and 12.5 μM), the expressions of a number of genes were found to be significantly changed. Gene ontology analysis indicated that the identified DEGs were primarily related to the mitotic cell cycle, chromosome, centromeric region and protein binding. Kyoto Encyclopedia of Genes and Genomes pathway analysis of the GP6.25 group revealed that pathways related to the cell cycle, oocyte meiosis, progesterone-mediated oocyte maturation and p53 signaling were the most changed, while that of the GP12.5 group revealed that pathways related to the PI3K-Akt signaling, focal adhesion, HIF-1 signaling, cell cycle and ECM-receptor interaction were the most changed. Genes associated with female reproductive function ( CDK1 , CCNB1 , CPEB1 and MMP3 ), cellular component organization ( BIRC5 , CYP1A1 , TGFβ3 and COL1A2 ) and oxidation-reduction processes ( PRDX6 , MGST1 and SOD3 ) were confirmed to be differently expressed in GP-treated groups. Conclusion: Our findings provide insight regarding changes in GC gene expression in porcine exposed to GP.
A Study of the Predictors of Breastfeeding Self-Efficacy in Mothers During the Covid-19 Pandemic
Authors: Beheshti Maryam Ahmad Zadeh , Alimoradi Zainab , Bahrami Nasim 
Published: February 2021

Abstract
Background and Purpose: Breastfeeding has a vital role in ensuring the health of mother and infant, and ultimately the health of society. Breastfeeding self-efficacy is a strong predictor of the duration of breastfeeding, which in a variety of situations - including crises - may be affected by a number of factors. The aim of this study is to determine the predictors of breastfeeding self-efficacy in breastfeeding mothers during the Covid-19 pandemic.Materials and Methods: A cross-sectional study was conducted with the participation of 300 breastfeeding mothers whose children were breastfed during the Covid-19 pandemic. Sampling was based on convenience sampling and online method using a checklist of personal and social characteristics and breastfeeding self-efficacy questionnaires, spouse postpartum social support, perceived social support, anxiety and depression, and fear of Covid-19. Data were analyzed using Pearson correlation coefficient, one-way ANOVA and multi variable linear regression model via stepwise method. Significance level in this study was considered to be 0.05.Results: The mean breastfeeding self-efficacy score among participants was 58.19±10.48 (out of 70). Spouse postpartum social support (β = 0.732, p =0.04), intention to breastfeed (p = 0.001, β = 0.17), use of formula while breastfeeding (β = - 0.09, p<0.001), and depression (β = - 0.11, p<0.001), were predictors of breastfeeding self-efficacy. But fear of Covid-19 was not significantly correlated with breastfeeding self-efficacy (p = 0.514).Conclusion: The results of the present study showed that spouse postpartum social support and having the intention of breastfeeding with a positive effect; and depression and simultaneous use of formula in feeding the infant with a negative effect; are predictors of women’s breastfeeding self-efficacy during the Covid-19 pandemic. It seems that breastfeeding can be improved by counseling to improve receiving the spouse support, increasing the breastfeeding intent, and reducing the depression.
ClusterMap: multi-scale clustering analysis of spatial gene expression
Authors: He Yichun , Tang Xin , Huang Jiahao , Zhou Haowen , Chen Kevin , Liu Albert , Ren Jingyi , Shi Hailing , Lin Zuwan , Li Qiang , Aditham Abhishek , Shu Jian , Liu Jia , Wang Xiao 
Published: February 2021

Abstract
Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation in complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles in intact tissues. However, there is a lack of a unified computational framework for integrative analysis of in situ transcriptomic data. Here, we present an unsupervised and annotation-free framework, termed ClusterMap, which incorporates physical proximity and gene identity of RNAs, formulates the task as a point pattern analysis problem, and thus defines biologically meaningful structures and groups. Specifically, ClusterMap precisely clusters RNAs into subcellular structures, cell bodies, and tissue regions in both two- and three-dimensional space, and consistently performs on diverse tissue types, including mouse brain, placenta, gut, and human cardiac organoids. We demonstrate ClusterMap to be broadly applicable to various in situ transcriptomic measurements to uncover gene expression patterns, cell-cell interactions, and tissue organization principles from high-dimensional transcriptomic images.
Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis
Authors: Matsumura Yoshihiro , Ito Ryo , Yajima Ayumu , Yamaguchi Rei , Magoori Kenta , Tanaka Toshiya , Kawamura Takeshi , Hirakawa Hiroyuki , Fujihashi Hitomi , Abe Yohei , Nakaki Ryo , Uchida Aoi , Yamamoto Shogo , Ota Satoshi , Tsutsumi Shuichi , Inoue Shin-ichi , Kimura Hiroshi , Wada Yoichiro , Inagaki Takeshi , Kodama Tatsuhiko , Osborne Timothy , Aburatani Hiroyuki , Node Koichi , Sakai Juro 
Published: February 2021

Abstract
Enhancer activation is essential for cell-type specific gene expression during cellular differentiation, however, how enhancers transition from a hypoacetylated “primed” state to a hyperacetylated-active state is incompletely understood. Here, we show SET domain-containing 5 (SETD5) forms a complex with NCoR-HDAC3 co-repressor that prevents histone acetylation of enhancers for two master adipogenic regulatory genes Cebpa and Pparg early during adipogenesis. The loss of SETD5 from the complex is followed by enhancer hyperacetylation. SETD5 protein levels were transiently increased and rapidly degraded prior to enhancer activation providing a mechanism for the loss of SETD5 during the transition. We show that induction of the CDC20 co-activator of the ubiquitin ligase leads to APC/C mediated degradation of SETD5 during the transition and this operates as a molecular switch that facilitates adipogenesis.
Microbiology Characteristics and Risk Factors of Surgical Site Infection in Limb Fractures: a Single-center Retrospective Study
Authors: Luo Junchao , Chen Xinji , Zhang Yin , Tong Yu , Zhu Senbo , Ji Lichen , Zhang Wei , Bi Qing , Zhang Qiong 
Published: February 2021

Abstract
Background: Limb fractures were becoming more common, and internal fixation increased the risk of surgical site infection. The purpose of this study was to identified risk factors for surgical site infection and the factors that affected the microbiological characteristics.Material and methods: Data from patients with limb fractures who received internal fixation between January 2010 and January 2020 were included. Univariate and multivariate logistic analyses were performed to determine independent risk factors.Results: A total of 4479 patients were identified and 187 (4.18%) patients developed surgical site infections (SSI). Staphylococcus aureus (25.1%) was the most common bacteria, followed by Coagulase negative Staphylococcus (18.8%) and Acinetobacter baumannii (12.0%). Multiple regression analysis revealed that low total protein (OR, 4.066; 95% CI, 1.217-13.583) and high white blood cell count (OR, 3.133; 95% CI, 1.291-7.605) were risk factors for gram-negative bacteria infection. Five risk factors were identified to be independently associated with the development of SSIs, including current smoking (OR, 2.923; 95% CI, 1.294-6.599), high-energy injury (OR, 2.816; 95% CI, 1.368-5.799), femoral fracture, tibia and fibula fracture, vascular injury at the fracture site (OR, 10.284; 95% CI, 2.506-42.199), skin contusin at the fracture site (OR, 3.348; 95% CI, 1.304-8.574), hypohemoglobin (OR, 2.991; 95% CI, 1.422-6.292).Conclusion: Preoperative preventative measures should be taken in patients at high risk to reduce the incidence of SSIs.
Trends in Organic Contamination in River Yamuna: A Case Study of Delhi Stretch (2007-2016)
Authors: Mazhar Mohd. Aamir , Ahmed Sirajuddin , Husain Azhar , uddin Rahis , Khan Nadeem A. 
Published: February 2021

Abstract
The river Ganges largest tributary is river Yamuna and it is the longest tributary in India serving millions of individuals. It is emerging from the glacier known as Yamunotri that has a height of 6,387m that travel through Uttarakhand to Allahabad. The water from river is abstracted as well as in stream used for irrigation, power generation, domestic water supply, industrial use, etc., because of which the after affects are many. In India, an alarming situation exists since quite a long time in river pollution. When river Yamuna enters Delhi, it meets the water quality guidelines with respect to Dissolved Oxygen (DO) and Biochemical Oxygen Demand (BOD) but during its exit the water quality deteriorated. The main reasons of deterioration of the river water are sewage discharge and industrial effluents and mis utilization of fresh water. The dilution capacity of the river also gets reduced due to significant water abstraction. The chief contributor of contamination is National Capital Territory (NCT) of Delhi followed by Agra and Mathura by either point or non-point sources.In this paper we investigated the ongoing trends in basic water quality guidelines of the River Yamuna which show huge deviation in Delhi segment. Due to the influence of industrialization, urbanization and horticultural advances the Delhi segment gets severely contaminated. Yamuna Action Plan (YAP) was undertaken by the government for the restoration and preservation of the river Yamuna. The DO, BOD in the Delhi segment and eutrophicated segment investigation, the water quality parameter trends in the river Yamuna represent that regardless of the considerable number of endeavors the water quality isn't fit for assigned best utilizations. The outcomes require inventive points of view in the advancement of a refreshed comprehensive preservation technique for the river Yamuna.
Energy Conversion and Storage: The Value of Reversible Power-to-Gas Systems
Authors: Glenk Gunther , Reichelstein Stefan 
Published: February 2021

Abstract
Power-to-Gas technology has recently experienced lower acquisition costs and lower conversion efficiency losses. At the same time, wholesale power markets have seen increasing volatility with significant amounts of surplus electricity at select hours of the year. Here we examine the economic potential of reversible Power-to-Gas systems that can convert electricity to hydrogen or operate in the reverse direction to deliver electricity during times of high power prices. Our model framework is applied to the current market environment in both Germany and Texas. We find that the reversibility feature of solid oxide fuel cells makes such systems already competitive at current hydrogen prices, provided the fluctuations in electricity prices are as pronounced as currently observed in Texas. We project that the flexibility inherent in reversible fuel cells would leave investments in such systems economically viable in the future even at substantially lower hydrogen prices, provided recent technological improvements continue over the coming decade.
Effect of Different Surface-charged Lamellar Materials on Swelling Properties of Nanocomposite Hydrogels
Authors: Pfeifer Marcela , Andrade Flávio A. C. , Bortoletto-Santos Ricardo , Aouada Fauze A. , Ribeiro Caue 
Published: February 2021

Abstract
This study investigated the effect of different surface-charged lamellar materials on the swelling and diffusion properties of synthesized polyacrylamide-methylcellulose hydrogels (HG). Montmorillonite and hydrotalcite thermally activated at two different temperatures (300 and 550 ºC) were incorporated in the preparation of nanocomposite (NC) hydrogels. A series of NC hydrogels were prepared by varying the lamellar material content (1:1, 2:1 and 4:1). The results showed that the HG with hydrotalcite (550 ºC) was strongly dependent on the ionic intensity, and that the swelling degree increased by 50%, 65% and 78% with reducing the hydrotalcite content at (1:1), (2:1) and (4:1), respectively. The water absorption capacity of HG containing montmorillonite or hydrotalcite (300 ºC) was slightly affected when the pH decreased from 7 to 3. However, the pH variation from 7 to 10 increased the water absorption capacity of most HG, except those containing hydrotalcite (550 ºC) at (2:1) and (4:1). The presence of lamellar nanoparticles in hydrogels made the polymer matrix more rigid, and less likely to absorb water. In contrast, HG with hydrotalcite (550 ºC) at (2:1) and (4:1) showed anomalous behavior with an increase in their water absorption capacity. The results support that the developed NC-HG can be suitable candidates for applications as controlled released materials.
Study by Absorption and Emission Spectrophotometry of the Efficiency of the Binary Mixture (Ethanol-Water) on the Extraction of Betanin from Red Beetroot
Authors: NOUAIRI Mohammed El-Amine , FREHA Mohammed , BELLIL Ali 
Published: February 2021

Abstract
In this paper, we intend to present a method of extraction and analysis of betanin from Algerian red beetroot, and to know the efficiency of the binary mixture (Ethanol-Water) on the extraction. This dye is a natural pigment with high antioxidant power and widely used as a natural dye. In addition, the high bioavailability of this pigment reinforces its potential for future therapeutic applications. However, the betanin content of commercially available beet extracts never exceeds 1.2% and this molecule does not exist marketed in a more purified form, it is therefore necessary to have efficient tools for the enrichment of betanin extracts. The experimental results made in our laboratory, confirm the effectiveness of the binary mixture (Ethanol-Water) on the extraction of betanin from Algerian beetroot.
Epichloë Fungal Endophyte Symbiosis May Improve Tall Fescue Responses to Flooding and Oxygen-Limited Conditions
Authors: Saedi Tayebeh , Mosaddeghi Mohammad Reza , Sabzalian Mohammad Reza , Zarebanadkouki Mohsen 
Published: February 2021

Abstract
Background and aims There is little information about the effect of grass-fungal endophyte symbiota on plant performance under oxygen-limited conditions. This study aimed to investigate the effect of Epichloë endophyte symbiosis and tall fescue genotype on plant responses to oxygen stress in a greenhouse pot experiment.Methods A greenhouse pot experiment was conducted with seven air-filled porosity levels in a sandy loam soil using two genotypes (75C and 75B) of tall fescue (Festuca arundinacea = Schedonorus arundinaceus Schreb.) infected with and without endophytic fungus Epichlöe coenophiala (E+ and E–, respectively). Some selected growth and physiological parameters were determined after nine-month application of the treatments.Results The results showed that E+ plants benefited from endophytic symbiosis and showed slightly higher root and shoot development, more leaf chlorophyll, and lower catalase and ascorbate peroxidase activity than E– plants under poor aeration. The E– plants also coped with poor aeration conditions by forming adventitious roots at the soil surface, aerenchyma formation within the root tissue, and increased alcohol dehydrogenase (ADH) activity.Conclusions The presence of endophyte improved the performance of the genotype E+ 75B under anaerobic conditions, while endophyte had an adverse effect on the performance of the genotype E+ 75C. In general, Epichloë endophyte presence decreased the flooding induced oxidative stress and prevented the formation and over-accumulation of reactive oxygen species in plant cells.
Interactions Between Rheumatoid Arthritis Antibodies Are Associated With the Response to Anti-tumor Necrosis Factor Therapy
Authors: Julià Antonio , López-Lasanta Maria , Blanco Francisco , Gómez Antonio , Haro Isabel , Mas Antonio Juan , Erra Alba , Vivar Maria Luz García , Monfort Jordi , Sánchez-Fernández Simón , González Isidoro , Alperi Mercedes , Castellanos-Moreira Raúl , Fernández-Nebro Antonio , Díaz-Torné César , Palau Núria , Lastra Raquel , Lladós Jordi , Sanmartí Raimon , Marsal Sara 
Published: February 2021

Abstract
Background: Blocking of the Tumor Necrosis Factor (TNF) activity is a successful therapeutic approach for 50-60% of rheumatoid arthritis (RA) patients. However, there are yet no biomarkers to stratify patients for anti-TNF therapy. Rheumatoid factor (RF) and anti-cyclic-citrullinated antibodies (anti-CCP) have been evaluated as biomarkers of response but the results have shown limited consistency. Anti-carbamylated protein (anti-CarP) and anti-peptidylarginine deiminase type 4 (anti-PAD4) antibodies have been much less studied. Despite being linked to common immune processes, the interaction between these markers has not been evaluated yet. Our aim was to analyze the interaction between these four antibodies in relation to the response to anti-TNF therapy.Methods: For this objective, a prospective cohort of n=80 RA patients starting anti-TNF therapy was recruited. Serum determinations at baseline were performed for RF, anti-CCP, anti-CarP and anti-PAD4 antibodies using enzyme-linked immunosorbent assays (ELISA). The clinical response to anti-TNF therapy was determined at week 12 using the change in DAS28 score. Association was performed using multivariate linear regression adjusting for baseline DAS28, sex and age. Results: The interaction between pairs of antibodies was tested by the addition of an interaction term. We found two highly significant antibody interactions associated with treatment response: anti-CarP with anti-PAD4 (p=0.0062), and anti-CCP with RF (p=0.00068). The latter antibody interaction was replicated in an independent retrospective cohort of RA patients (n=199, p=0.04). Conclusions: The results of this study suggest that antibody interaction effects are important factors in the response to anti-TNF therapy in RA.