bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing Environmental Sustainability and Socio-Economic Prosperity: The Feasibility of the AMIS Methodology in Mitigating H2S Impacts in Ulumbu Geothermal Working Area
Authors: Ahmad D.M , Akhlaqul D. 
Published: February 2025

Abstract
Geothermal energy is one of Indonesia’s national strategic programs to reduce the country’s dependence on fossil fuels. Geothermal energy also plays an important role in improving the economic conditions of communities in the surrounding area. However, this economic growth has also been accompanied by negative impacts on the environment. The dispersion of hydrogen sulfide (H2S) gas has been a major concern in the Ulumbu Geothermal Working Area (WKP), Manggarai Regency, East Nusa Tenggara (NTT) since it developed in 2012. This research aims to explore the feasibility of AMIS (Abbattimento Mercurio ed Idrogeno Solforato) methodology to mitigate the impact of H2S. The research also emphasizes the importance of careful planning by project developers and local government to achieve a balanced approach between methodological advancement and environmental preparedness. By combining methodological refinement with careful planning, this study highlights the good potential of the AMIS methodology in mitigating H2S impacts. This convergence of approaches not only promotes environmental sustainability but also revitalizes agricultural and plantation activities and advances the socio-economic welfare of the Ulumbu area community.
Pressure Prediction in Two-Phase Geothermal Well Flow Using Physics Informed Neural Network (PINN)
Authors: Aji Nugroho Agung , Sutopo  , Ashat Ali 
Published: February 2025

Abstract
To ensure optimal production from geothermal wells, it is essential to simulate fluid behavior accurately in the reservoir and wellbore. Wellbore simulation uses mathematical models to predict the pressure drop in fluid flow from the bottom hole to the wellhead. However, it is essential to understand that using numerical simulators still has limitations due to inappropriate empirical equations and unrealistic assumptions. Artificial Neural Network (ANN) can learn the correlation between input (wellbore geometry, temperature, pressure, and mass flow rate) and output variables (pressure drop) well. ANN is very promising for solving problems in the field of science that involve processes that are not fully understood or that require complex computational processes. However, the application of ANN in the science domain has limitations because it requires extensive data, the inability to produce physically consistent predictions, and the lack of generalization to scenarios outside the training data. Physics-Informed Neural Network (PINN) integrates ANN and scientific knowledge to overcome inconsistent physical parameters with training data that is not fully representative. We will discuss the physics loss function used to produce the PINN model with the best wellbore simulation. Overall, the PINN model with physics loss functions corrected by temperature can provide more accurate pressure predictions in the wellbore and fewer errors than the ANN model. The total error produced by the PINN model for five wells is MAE 2.780 and RSME 3.151. However, the PINN model that has been developed is not suitable for use in the one-phase water zone.
What's Wrong with Stereotyping?
Authors: Beeghly Erin 
Published: February 2025

Abstract
What’s Wrong with Stereotyping? offers a groundbreaking, accessibly written philosophical account of the ethics of stereotyping. The book advances a complex and often surprising notion of what stereotyping is, and when and why it is wrongful. Using philosophy, law, psychology, and history, as well as thought-provoking examples and models for social change drawn from the lived experiences of marginalized groups, the book emphasizes the messiness of moral reality and the importance of looking to the past to understand the ethical perils of social generalizing. Readers come away with a radically pluralistic, open-ended understanding of stereotyping that they can use to identify wrongful stereotyping in their own lives and our contemporary world.
Introduction
Authors: Beeghly Erin 
Published: February 2025

Abstract
This introduction familiarizes readers with the idea of an explanatory project in ethics and, in doing so, invites them to contemplate the ethical puzzles associated with stereotyping. Is stereotyping always morally wrong, as people often believe? Is it only sometimes wrong? What explains why it is wrong when it indeed is? This is the puzzle of stereotyping. Moving chapter by chapter, this introduction sketches the overall shape of book’s argument so that readers can better orient themselves as they read, explains its down-to-earth methodology, and previews the radically pluralistic theory of wrongful stereotyping for which it argues.
A Starting Point for Theorizing
Authors: Beeghly Erin 
Published: February 2025

Abstract
This chapter examines stereotypes and stereotyping from linguistic, psychological, and sociological perspectives. Investigating the psychology of stereotypes and stereotyping, it examines their connection to generalization and induction, core aspects of human rationality. Building on the work of Patricia Hill Collins, it also contends that cultural stereotypes take the form of “controlling” ideas and images. To capture stereotyping in all its manifestations and emphasize their interconnection, the chapter introduces morally neutral definitions that don’t build wrongness into the very idea of what it means to stereotype. To stereotype a group, it says, is to characterize that group as a collective entity. To stereotype an individual is to judge that person by group membership. These definitions don’t sweep power relations under the rug. Rather, they recognize that wrongful stereotyping involves the social hijacking of generalizing tendencies that are not inherently immoral and which in fact are essential to human functioning.
Prejudice and the Problem of Statistical Stereotyping
Authors: Beeghly Erin 
Published: February 2025

Abstract
This chapter explores prejudice-focused objections to stereotyping in the testimonies of American AIDS activists in the 1980s and 1990s. It begins with classic prejudicial stereotyping, a flagrant form of wrongful stereotyping that involves false morally vicious attitudes. I then examine a putative limitation of prejudice-focused critiques. Not all discriminators—and not all people who stereotype—have vicious attitudes. Statistical stereotyping is a case in point. Exploring a realistic example of medical statistical stereotyping critiqued by feminist AIDS activists, the chapter highlights the philosophical limits of prejudice-focused critiques. At the same time, it contends that any theory of wrongful stereotyping that ignores prejudice will be both incomplete and misleading. Though prejudice may not always explain what’s morally wrong with stereotyping, it sometimes does, revealing a distinctive way in which acts of stereotyping can wrong individuals, and the groups to which they belong, by disrespecting them.
Gesture
Authors: Gawne Lauren 
Published: February 2025

Abstract
The gestures that we use when we speak are an important, if often overlooked, part of how we communicate. This book provides a friendly, fast-paced introduction to the field of Gesture Studies. Gestures are those communicative actions made with the human body that accompany spoken or signed language. Paying attention to gesture means paying attention to the fuller context in which humans communicate. Gesture is absolute, in that every human community that has language also has gestures as part of that language. But gesture is also relative, in that it is far more heavily context dependent than other elements of communication. This book provides a broad introduction to current understandings of the nature and function of gesture as a feature of communication. This Slim Guide covers the ways gesture works alongside speech and the different categories of gesture. The way these categories are used varies across cultures and languages, and even across specific interactions. We acquire gesture as part of language, and it is deeply entwined with language in the brain. Gesture has an important role in the origin of language, and in shaping the future of human communication. The study of gesture makes a crucial interdisciplinary contribution to our understanding of human communication. This Slim Guide provides an introduction to Gesture Studies for readers of all backgrounds.
Discovery and Phase 1 study of a novel monoclonal antibody against human IL-1β for the treatment of IL-1β-mediated diseases
Authors: Cho Minseon , Tam Susan H , Shi Lihua , Fung Isa , Tornetta Mark , Canziani Gabriela A , Fung Man-Cheong , Chiu Mark L , Han Chao , Zhang Di 
Published: February 2025

Abstract
Interleukin-1β (IL-1β) is a key mediator of innate immunity against pathogen infections. However, dysregulated IL-1β activity is associated with various autoinflammatory, autoimmune, degenerative, atherosclerotic diseases, and cancers. Biologic drugs that neutralize excess IL-1β activity, such as canakinumab, have been effective in treating IL-1β-mediated diseases. This article reports the discovery and development of a novel humanized anti-IL-1β antibody, designated as TAVO103A, which exhibited potent binding affinities to human and monkey IL-1β. TAVO103A demonstrated more potent neutralization of IL-1β activities compared to canakinumab in multiple assays, including tests on the IL-1β-driven signal transduction cascade, inflammatory cytokine release from MRC-5 cells, chemokine release from A549 cells, and the proliferation of D10.G4.1 helper T cells. Ex vivo studies showed that TAVO103A effectively neutralized IL-1β-mediated release of pro-inflammatory cytokines from peripheral blood mononuclear cells (PBMC). In addition, TAVO103A exhibited dose-dependent efficacy in a knee joint inflammation mouse model. TAVO103A underwent Fc engineering to reduce binding to Fcγ receptors, increase affinity to FcRn receptors, and enhance its resistance to proteolytic degradation. In a Phase 1 study, TAVO103A was found to be safe, well tolerated, and demonstrated a median half-life of 63 days in healthy subjects. By recognizing a different epitope, TAVO103A provided more potent neutralization of IL-1β activities, a longer circulating half-life, and improved safety profiles compared to canakinumab, positioning it to be a potential best-in-class therapeutic option for various IL-1β-mediated diseases.
A case report of cardiogenic shock due to tachycardic atrial fibrillation: a life-saving catheter ablation for atrial fibrillation and prone position during veno-arterial extracorporeal membrane oxygenation
Authors: Wakita Masaki , Hosokawa Yusuke , Ishihara Shiro , Maruyama Mitsunori , Asai Kuniya 
Published: February 2025

Abstract

Background
Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) is vital for acute cardio-respiratory failure, but challenges persist sometimes in weaning. Prone positioning, beneficial in veno-venous extracorporeal oxygenation (VV-ECMO), is generally avoided in VA-ECMO patients because of the risks such as bleeding from the catheter insertion site and dislodging of the perfusion cannula or tracheal tube.

Case Summary
A 71-year-old male with paroxysmal atrial fibrillation (AF) presented with dyspnea. Chest computed tomography (CT) and echocardiogram revealed severe pneumonia and decreased cardiac function (left ventricular ejection fraction (LVEF) 20%). The patient was diagnosed with heart failure complicated by severe pneumonia. Despite treatment, respiratory and circulatory status deteriorated, necessitating a VA-ECMO. Chest CT showed collapsed lungs, especially on the dorsal side. Thus, prone positioning was initiated under VA-ECMO with attention to potential complications associated with the postural change. Then, his respiratory status was improved dramatically. However, the hemodynamic status could not be probably because of tachycardic AF. Electrical cardioversions failed to maintain sinus rhythm and heart rate was not adequately controlled. We decided to perform radiofrequency catheter ablation (RFCA) of tachycardic AF under VA-ECMO. Pulmonary vein isolation and conduction block line in the cavo-tricuspid isthmus were made, and converted to sinus rhythm, enabling weaning of VA-ECMO. The primary cause of the hart failure was considered to be tachycardia-induced cardiomyopathy, as the LVEF improved to 55%.

Discussion
RFCA for AF can be effective even in case of cardio-respiratory failure necessitating VA-ECMO. Furthermore, prone positioning can be performed not only in VV-ECMO but also in VA-ECMO.
Experimental evolution reveals trade-offs between sexual selection and heat tolerance in Drosophila prolongata
Authors: De Nardo Alessio N , Meena Abhishek , Maggu Komal , Eggs Benjamin , Sbilordo Sonja H , Lüpold Stefan 
Published: February 2025

Abstract
Sexual selection promotes traits that enhance mating or fertilization success, but these traits can be very costly under harsh environmental conditions. The extent to which differential investment in costly traits under varying intensities of sexual selection is related to their susceptibility to environmental stress remains unclear. This study explored how experimental evolution under different operational sex ratios (OSRs) shapes traits and reproductive success of male Drosophila prolongata, and how developmental and/or adult heat stress affect the expression of these traits. We found males from even and slightly male-biased OSRs to be larger and display greater reduction in body size under developmental heat stress, suggesting pre-mating sexual selection on body size and condition-dependent thermal sensitivity. These populations also exhibited consistently high mating and fertilization success across temperatures, potentially indicating selection for robust phenotypes with ‘good genes’ that perform well regardless of temperature. Conversely, males from strongly male-biased OSR populations experienced more pronounced decline in sperm competitiveness following exposure to developmental or adult heat stress. These results highlight how environmental stressors differentially impact populations, shaped by varying strengths of pre- and post-mating sexual selection. These observed patterns suggest potential interactions between past selection and the ability to adapt to changing environments.
The nasal microbiota of two marine fish species: diversity, community structure, variability and first insights into the impacts of climate change-related stressors
Authors: Cohen-Rengifo Mishal , Noel Cyril , Ytteborg Elisabeth , Bégout Marie-Laure , Lazado Carlo C , Le Blay Gwenaelle , Hervio-Heath Dominique 
Published: February 2025

Abstract
Vertebrate nasal microbiota (NM) plays a key role regulating host olfaction, immunity, neuronal differentiation, and structuring the epithelium. However, little is known in fish. This study provides the first comprehensive analysis of the NM in two marine fish species, the European seabass and the Atlantic cod. Given its direct environmental exposure, fish NM is likely influenced by seawater fluctuations. We analysed the community structure, specificity regarding seawater, and interindividual variability of 32 to 38 fish reared under ambient conditions. Additionally, we conducted an experiment to investigate the influence of acidification and a simplified heatwave on cod NM (3 fish per replicate). High-throughput 16S rRNA sequencing revealed species-specific NM communities at the genus-level with Stenotrophomonas and Ralstonia dominating seabass and cod NM, respectively. This suggests potential habitat- or physiology-related adaptations. The most abundant bacterial genera in seabass NM were also present in seawater, suggesting environmental acquisition. Alpha diversity was highest in Brest seabass NM and variability greatest in Tromsø cod NM. Simulated climate change-related scenarios did not significantly alter cod NM structure. We propose a minimum of 13 cod rosettes per replicate for future studies. This research establishes a foundation for understanding marine fish NM and its response to environmental changes.
Tea polyphenol mediated CsMYB77 regulation of CsPOD44 to promote tea plant (Camellia sinensis) root drought resistance
Authors: Xu Rong , Shao Chenyu , Luo Yuqi , Zhou Biao , Zhu Qian , Qiu Shuqi , Liu Zhonghua , Liu Shuoqian , Shen Chengwen 
Published: February 2025

Abstract
Drought stress significantly alters the metabolic homeostasis of tea plants; however, few studies have examined the role of specific metabolites, particularly tea polyphenols, in drought resistance. This study reveals that the tea polyphenol content in drought-tolerant tea cultivars tends to increase under drought conditions. Notably, in environments characterized by staged and repeated drought, changes in tea polyphenol are significantly positively correlated with drought resistance. To investigate this further, we irrigated the roots with exogenous tea polyphenols before subjecting the plants to drought. Our findings indicated that the absorptive roots of the experimental group exhibited enhanced development, improved cellular integrity, and a significant increase in peroxidase activity. A comprehensive analysis of the transcriptome and metabolome revealed that tea polyphenols are closely associated with the phenylpropanoid metabolism pathway. Notably, CsMYB77 and CsPOD44 genes were identified as highly correlated with this pathway. Overexpression experiments in Arabidopsis thaliana demonstrated that CsMYB77 promotes the expression of phenylpropanoid pathway genes, thereby enhancing drought resistance. Conversely, antisense oligonucleotide silencing of CsMYB77 decreased drought resistance in tea plants. Additional experiments, including yeast one-hybrid assays, luciferase complementation imaging, dual-luciferase assays, and electrophoretic mobility shift assays, confirmed that CsMYB77 positively regulates the expression of CsPOD44. In summary, our findings indicate that the differences in drought tolerance among tea cultivars are closely linked to phenylpropanoid metabolism. Specifically, tea polyphenols may mediate the regulatory network involving CsMYB77 and CsPOD44, thereby enhancing stress resistance by promoting root development. This study offers new insights into the breeding of drought-resistant tea cultivars.
Aggregatin is a mitochondrial regulator of MAVS activation to drive innate immunity
Authors: Gao Ju , Ding Mao , Xiyang Yanbin , Qin Siyue , Shukla Devanshi , Xu Jiawei , Miyagi Masaru , Fujioka Hisashi , Liang Jingjing , Wang Xinglong 
Published: February 2025

Abstract
Mitochondrial antiviral-signaling protein (MAVS) is a key adapter protein required for inducing type I interferons (IFN-Is) and other antiviral effector molecules. The formation of MAVS aggregates on mitochondria is essential for its activation; however, the regulatory mitochondrial factor that mediates the aggregation process is unknown. Our recent work has identified the protein Aggregatin as a critical seeding factor for β-amyloid peptide aggregation. Here we show that Aggregatin serves as a cross-seed for MAVS aggregates on mitochondria to orchestrate innate immune signaling. Aggregatin is primarily localized to mitochondria in the cytosol and has the ability to induce MAVS aggregation and MAVS-dependent IFN-I responses alone in both HEK293 cells and human leukemia monocytic THP-1 cells. Mitochondrial Aggregatin level increases upon viral infection. Also, Aggregatin knockout suppresses viral infection-induced MAVS aggregation and IFN-I signal cascade activation. Nemo-like kinase is further identified as a kinase phosphorylating Aggregatin at Ser59 to regulate its stability and cross-seeding activity. Collectively, our finding reveals an important physiological function of Aggregatin in innate immunity by cross-seeding MAVS aggregation.
Targeting PGE2 mediated senescent neuron improves tumour therapy
Authors: Zhao Jianyi , Wu Linshi , Cai Gang , Ou Dan , Liao Keman , Yang Jian , Zhou Li , Huang Renhua , Lin Shukai , Huang Xi , Lv Qi , Chen Juxiang , Cao Lu , Chen Jiayi , Lin Yingying 
Published: February 2025

Abstract

Background
Recent studies have highlighted bidirectional signalling between tumours and neurons; however, the interactions between tumours and neurons in response to radio-/chemotherapy remain obscure, which hampers the tumour treatment.

Methods
Glioblastoma organoids (GBOs) and primary neuron coculture, targeted metabonomics, RNA pulldown, mass spectrum, co-immunoprecipitation, RNA-sequencing, transcript/protein validations and multi-electrode arrays were performed to analyse neuron-tumour interaction in response to therapy. In vivo validations were conducted in orthotopic mouse models. Diagnostic and prognostic values were evaluated in serum, tissue-microarray as well as TCGA.

Results
GBOs recruited and induced pro-tumour-survival senescent neurons upon radiation/chemotherapeutic treatment. Targeted metabonomics revealed that significantly increased tumour-derived prostaglandin E2 (PGE2) induced neuronal senescence phenotype. Screening of enzymes involved in PGE2 synthesis identified prostaglandin E synthase 3 (PTGES3) as the key enzyme responsible for PGE2 upregulation. Biochemical studies revealed that irradiation or chemotherapeutic drug-triggered asparagine endopeptidase (AEP) specifically cleaved eIF4A1 to produce teIF4A1-C, which dissociated from DDX6 and recruited eIF4A3 and PABPN1 to promote the mRNA stability of PTGES3. Elevated PGE2 reciprocally enhanced AEP expression. Inhibiting PGE2 or AEP reduced neuronal senescence and delayed tumour progression. Strikingly, single-cell analysis further showed that expressions of AEP/PTGES3/EIF4A1 in tumour cells were consistent with senescent neuronal CDKN1A in high-neuronal-connectivity glioblastoma. The serum PGE2 concentration was elevated after radiation and higher in resistant glioblastoma patients. High expression of PTGES3 was associated with a poor prognosis.

Conclusions
Our study revealed that the AEP/PGE2 feedback loop modulates tumour-induced neuronal senescence upon radio-/chemotherapy and highlight the therapeutic value to improve tumour therapy.