bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complement C4 is Reduced in iPSC-Derived Astrocytes of Autism Spectrum Disorder Subjects
Authors: Mansur Fernanda , Silva André Luiz Teles e , Santos-Gomes Ana Karolyne , Magdalon Juliana , de Souza Janaina Sena , Griesi-Oliveira Karina , Passos-Bueno Maria Rita , Sertie Andrea Laurato 
Published: March 2021

Abstract

In recent years, accumulating evidence has shown that the innate immune complement system is involved in several aspects of normal brain development and in neurodevelopmental disorders, including autism spectrum disorder (ASD). Although abnormal expression of complement components was observed in post-mortem brain samples from individuals with ASD, little is known about the expression patterns of complement molecules in distinct cell types in the developing autistic brain. In the present study, we characterized the mRNA and protein expression profiles of a wide range of complement system components, receptors and regulators in induced pluripotent stem cell (iPSC)-derived neural progenitor cells, neurons and astrocytes of individuals with ASD and neurotypical controls, which constitute in vitro cellular models that recapitulate certain features of both the human brain development and ASD pathophysiology. We observed that all the analyzed cell lines constitutively express several key complement molecules. Interestingly, using different quantification strategies, we found that complement C4 mRNA and protein are expressed in significantly lower levels by astrocytes derived from ASD individuals compared to control. As astrocytes participate in synapse elimination and diminished C4 levels have been linked to defective synaptic pruning, our findings may contribute to an increased understanding of the atypically enhanced brain connectivity in ASD.
Mono-crystalline nanometer high cristobalite glass-ceramic from nominal pyroxene glass
Authors: EL-Rafei Amira , Azoz Moenis A , Hamzawy Esmat M A 
Published: March 2021

Abstract
Transparent glass was prepared from nominal alkali pyroxene (Na2 Mg Si2O6) composition. Incorporation of Cr2O3 in alkali pyroxene composition in two % (0.5 and 1.0) increases the crystallization temperature. High cristobalite was developed alone in all glass samples heat-treated at 800°C. The microstructure shows nanometer crystals of high cristobalite which spread in glassy matrix. The densities, hardness and coefficient of thermal expansion were between 2.49 and 2.53 g/cm3, 505 and 611 kg/mm2 and between 9.38 and 13.32 x 10− 6°C− 1 (30–300 ˚C ) respectively. The antimicrobial activity of the prepared glass and ceramics shows that the only samples contain 1% of Cr2O3shows an inhibition zone in case of Candida albicans yeast.
N6-Methyladenosine methylome landscape and a novel m6A-related gene risk signature associated with the prognosis of glioblastoma
Authors: Zhao Rongrong , Li Boyan , He Zheng , Zhang Shouji , Guo Qindong , Zhang Ping , Qiu Wei , Qi Yanhua , Zhao Shulin , Pan Ziwen , Guo Xing , Xue Hao , Li Gang 
Published: March 2021

Abstract
Background

N6-Methyladenosine (m6A) represents one of the most prevalent mRNA modification, strongly linked to the initiation and progression of glioblastoma (GBM). However, the global transcriptome-wide patterns of m6A in GBM are largely unknown.
Methods

RNA m6A modification patterns in GBM and normal brain tissues were described via m6A-sequencing (m6A-seq). Least absolute shrinkage and selection operator (LASSO) regression and multivariate Cox regression analyses were performed to identify genes for constructing an m6A-related gene risk signature. A nomogram was developed by integrating the risk signature with clinicopathological factors. Time-dependent receiver operating characteristic (ROC) curves were used to evaluate the performance of the prognostic model.
Results

Here, we reported transcriptome-wide m6A modification profiling in GBM and normal brain tissues for the first time, illuminating highly differences between these two groups, showing that m6A regulates large number of genes and cancer-related pathways. We demonstrated an m6A-regulated 8-gene risk signature that correlated with poor prognosis of GBM patients from the TCGA dataset, and validated its efficiencies in LeeY, Gravendeel and CGGA datasets. Furthermore, we developed a nomogram that integrated the risk signature with clinicopathological factors and validated its better performance for predicting the 1-, 3- and 5-year survival rates of GBM patients.
Conclusion

This study presents the first m6A transcriptome-wide map of human GBM and normal brain tissues, whose m6A landscape is greatly altered in GBM; In addition, an epigenetic m6A-related 8-gene risk signature and a nomogram are established, whose efficiencies have a high potential to further improve the individualised therapy in GBM patients.
Effects of short-term breathing exercises on respiratory recovery in patients with COVID-19- a quasi-experimental study
Authors: Kader Manzur , Hossain Md. Afzal , Reddy Vijayendar , Perera Nirmala K. Panagodage 
Published: March 2021

Abstract
Background:Coronavirus disease 2019 (COVID-19) is a highly infectious respiratory tract disease. The most common clinical manifestation of severe COVID-19 is acute respiratory failure. Respiratory rehabilitation can be a crucial part of treatment but data lack for patients with COVID-19. This study investigates the effects of short-term respiratory rehabilitation (i.e., breathing exercises) on respiratory recovery among hospitalised patients with COVID-19. Methods: This quasi-experimental, pre-post-test study recruited 110 patients hospitalised with COVID-19. All patented received standardised care, and 65 patients also received the intervention (i.e., breathing exercises). Data on peripheral oxygen saturation (SpO2), respiratory rate (breaths/minute) and heart rate (beats/minute) and oxygen therapy requirement (litre/min) were collected at baseline and 4-5 days after the baseline assessment. Analysis of variance on repeated measures was applied to compare the outcomes of two-time points. Results: The mean (±Standard deviation, SD) age of the intervention group was 49.5 (±10.4) years and 73.8% were men. The mean (±SD) age of the control group was 49.3 (±7.9) years and 62.2% were men. After 4-5 days of respiratory rehabilitation SpO2 (96.7% ±2.1 vs 90.4% ±1.5), P<0.01), respiratory rate (20.5 ±2.4 vs 23.0 ±2.2) breaths/minute, P<0.01), heart rate (80.5 ±9.2 vs 91.2 ±8.6) beats/minute, P<0.01), and oxygen therapy requirement (0.4 ±0.98 vs 1.4 ±2.0) litre/min, P<0.01) improved in the intervention group compared to the control group. The mean days of hospitalisation for the intervention group and the control group were 7.1 days vs. 14.6 days, respectively. Conclusions:Our results indicate that breathing exercise, even for a short period, is effective in improving certain respiratory parameters in patients with COVID-19. As a non-invasive and cost-effective respiratory rehabilitation intervention, breathing exercise can be a useful tool for a health care system overwhelmed by COVID-19 pandemic. These results should be considered preliminary until they are replicated in larger samples in different settings.
Acquired Immunodeficiency Syndrome Presented as Atypical Ocular Toxoplasmosis: a Case Report
Authors: Pour Elias Khalili , Riazi-Esfahani Hamid , Ebrahimiadib Nazanin , Esteghamati Violet Zaker , Zarei Mohammad 
Published: March 2021

Abstract
Background: The aim is to report an atypical presentation of ocular toxoplasmosis which led to the diagnosis of Acquired Immunodeficiency Syndrome (AIDS). Case report: The 38-year-old woman was referred with metamorphopsia and reduced vision in the right eye in the past 3 weeks. Slit-lamp examination revealed granulomatous keratic precipitates (KPs), 2+ cells in the anterior chamber, and posterior synechiae. Fundus examination was remarkable for a white patch surrounding a scar, inferonasal to the optic disc with fibrous bands were emanating from the lesion and the retina around this region was detached with considerable extension towards periphery, while no breaks could be appreciated. She mentioned anorexia and losing 10 kg in the past three months and bilateral angular cheilitis was observed on systemic evaluation. The results of the patient's complete blood count revealed bicytopenia and CD4 lymphocytes: 79 cells/µl. Enzyme-linked immunosorbent assays (ELISA) for HIV antibodies came back positive which was later confirmed with the Western-blot test. Brain Magnetic Resonance Imaging (MRI) showed multiple ring-enhancing lesions in both cerebral cortices. Patient underwent Anti-Toxoplasmosis and Anti-HIV treatment and serous retinal detachment was completely resolved. Conclusion: This report highlights the fact that sometimes the eyes are the site of the first presentation of a systemic life-threatening condition and emphasizes the role of ophthalmologists in such cases. In cases of atypical presentation, appropriate laboratory tests and CNS imaging should be requested. Systemic treatment with anti-toxoplasmosis regimens and HAART is mandatory in AIDs patients with ocular toxoplasmosis.
Promotion and Potential Analysis of Biogas Plants to Produce Compressed Biomethane Gas for Transportation in Thailand
Authors: tonrangklang pennapa , Theryothin Apichit , Preechawuttipong Itthichai 
Published: March 2021

Abstract
BackgroundThe implementation of activities agriculture resulted in a large amount of wastewater and organic waste. In the past, Thailand has been using these to produce biogas to replace fossil fuels in the country. At present, biogas production has a residual amount due to no use in farms/factories. So, it to be released into the atmosphere. The government has a policy of biogas by the upgrade to produced biomethane for fuel vehicles but it is still just a prototype system. Therefore, if the government has targeted of the development biomethane in commercial plants, it is necessary to analyze the financial feasibility to an incentive of investors and plan for energy effective support. Including, people can use gas at a reasonable price and create a comprehensive energy source in sustainable agriculture.ResultsThe research evaluates the commercial investment potential of compressed biomethane gas plants based on upgrading biogas by water scrubber technology. The results identified biogas plants must have a digester volume of 2,000 m3 or more to produce compressed biomethane gas. Locations of 10 plants with the potential to establish compressed biomethane gas plants in Thailand. The capacity of compressed biomethane gas plants at 3, 6, 12 and 24 tons/day with no subsidy, subsidy 20% and 30% from the government. The plant should have a capacity of 12 tons/day or more in on subsidy from the government that there will be a financial possibility. The government should be subsidy at 30% in the plants of capacity at 3 tons/day for small plants can operate their businesses, subsidy 20% in the plants of capacity at 6 tons/day. In the part of plants more 12 tons/day which the government should be added measures such as Measures for low-interest loans, tax relief measures, etc. In addition, 10 locations will be expected to reduce greenhouse gas emissions by about 78,333 tCO2e/year. Conclusion﻿This study can be used to support decision-making on commercial investment in clean technology to promote the use of resources in agriculture for efficient transportation and support energy-efficient plans to provide an alternative sustainable fuel source to reduce global warming.
Algorithm for calculating parameters of mold temperature controller
Authors: Hong Liu , Danqi Pan , Zheqi Jiang 
Published: March 2021

Abstract
This paper presents a method to calculate the parameters of mold temperature controller based on the fast algorithm of mold water heating with these problems that it is difficult to calculate the parameters of injection mold temperature controller and it can’t be simulated. According to the requirements of fast mold water heating algorithm, firstly, similar transformations are used to construct a similar model to obtain a similar model with the same solution of the fluid-solid coupling mathematical model of mold water heating. The temperature field of the mold after heating is obtained through rapid simulation of the similar model. Secondly, a constrained mold temperature control equation is established, and the iterative method is used to numerically solve the mold temperature equation to obtain the mold temperature controller parameters that meet the heating conditions. An example is given in this paper to show that the calculation method of mold temperature controller parameters presented in this paper has good practical value.
Community Characteristics of Sympatric Freshwater Turtles From Savannah Waterbodies in Ghana
Authors: Gbewaa Suleman B. , Oppong S. Kobby , Horne Brian D. , Tehoda Paul , Petrozzi Fabio , Dendi Daniele , Akani Godfrey C. , Vittorio Massimiliano Di , Ajong Stephanie N. , Pacini Nic , Fa Julia E. , Luiselli Luca 
Published: March 2021

Abstract
Despite increasing pressures on freshwater resources worldwide, and the threatened status of most freshwater turtles, there is still limited knowledge of habitat use and niche partitioning in Afrotropical freshwater turtle communities. In this study, we describe habitat associations, community diversity, and temporal patterns of occurrence of freshwater turtle species in the Dahomey Gap ecoregion of Ghana (West Africa). We gathered data from 13 sites in central Ghana and along the Sene Arm of Lake Volta in the Digya National Park (Bono East Region). We employed opportunistic short-term surveys (at seven sites) together with longer-term (six-months duration) standardized evaluations of turtle presence and numbers in different habitats (at six sites). In addition, we interviewed fishers in the Lake Volta area to explore their perception about turtle abundance trends. Overall, 210 turtle individuals belonging to four species (Trionyx triunguis, Cyclanorbis senegalensis, Pelomedusa sp. and Pelusios castaneus) were recorded; for 139 individuals the precise capture sites and habitat type were recorded, whereas 71 individuals were observed in market places and were not considered in our analyses. We observed three sympatric species at the local scale of the various study sites. In each site, the dominant species was either C. senegalensis or Pelomedusa sp. However, Pelomedusa sp. was the most abundant species in temporary waterbodies whereas C. senegalensis was more numerous in permanent ones. A Multiple Correspondence Analysis visualized that, in permanent waterbodies all species were associated with similar physical habitat variables. C. senegalensis and T. triunguis were more abundant in localities containing woody emergent vegetation and inorganic substrate. In a Canonical Correspondence Analysis, we show that the density of herbaceous emergent vegetation was more important for P. castaneus than for C. senegalensis. Interviews with local people suggested that freshwater turtles do not have any special marketing interest for them, but that overfishing may have considerably affected the population density of these semiaquatic reptiles. Overall, Comparisons of diversity metrics between our study areas and previous literature revealed that turtle community composition was substantially the same, both qualitatively and quantitatively, all throughout the savannahs of West Africa.
Core decompression versus nonvascularized bone grafting in patients with osteonecrosis of femoral head
Authors: Mei Jin , Pang Lili , Jiang Zhongchao 
Published: March 2021

Abstract
Background
Core decompression and nonvascularized bone grafting are used in the early stage of osteonecrosis of femoral head for a period. Since the comparison of the core decompression and nonvascularized bone grafting are less reported, the purpose of our study was to investigate the clinical outcomes of two procedures in patients with earlier stages of the osteonecrosis of femoral head.
Methods
Between January 2018 and January 2019, 46 patients (48 hips) are divided into core decompression group and non-vascularized allogeneic fibula grafting group according to their procedures. The mean follow-up were 28.31±3.7 months (24–36 months) and 29.23±4.7months(24–36 months) respectively. the primary outcomes were VAS and HHS. Survivorship was analyzed with the collapse of femoral head or conversion to total hip arthroplasty (THA) as the endpoint.
Results
At the final follow-up, two hips underwent THA in core decompression group and three hips in non-vascularized allogeneic fibula grafting group. The radiographic survival rates are 76.9% and 77.3% in the two groups. Statistically significant improvements were seen in both groups on the VAS and HHS. The radiographic survival rate of hips was significantly inferior in lateral type lesion (P = 0.03; CI,0.765–2.114) and ARCO III stage (P = 0.28; CI, 1.254–58.752) in core decompression group. ARCO III stage (P = 0.02; CI, 1.351–49.344) and BMI>25 (P = 0.35; CI, 0.999–2.131) were independent risk factors for the collapse of femoral head in non-vascularized allogeneic fibula grafting group.
Conclusion
In this study, two procedures all can reduce the patient’s pain and improve functional activity. Arco stage III is a common risk factor for the collapse of femoral head in both groups. lateral lesion and BMI>25 are risk factors for the collapse of femoral head in core decompression group and non-vascularized allogeneic fibula grafting group, respectively. Both groups had good hip survival rates during the follow-up period.
Clinical Characteristics and Outcomes of Patients with Coronavirus Disease 2019-associated ARDS and the Early Intubation: A Two-Hospital Retrospective Cohort Study in Hubei, China
Authors: Mu Jing-Lan , Qin Ming-Zhe , Sun Hui-Mei , Guo Bao-Sheng , Qiu Shi-Da , Zhang Fu , Yang Huan , Nie Juan , Liu Xu-Dong , Yeung Wing-Fai , Liu Xiao-Dong , Li Bi-Xi , Song Xiao-Yang , Lin Shao-Yan , Dong Ye-Hong , Liu Ke-Xuan 
Published: March 2021

Abstract
Background: More evidence in understanding the heterogeneity of COVID-19-associated acute respiratory distress syndrome (ARDS) and in improving strategy to increase the survival from the critical patients intubated is always needed. The study aimed to comprehensively explore the features of COVID-19-associated ARDS and the features and outcomes between the early and late intubation groups. Methods: This retrospective cohort included 65 adult COVID-19 inpatients with ARDS at two hospitals in Hubei, China. The ARDS in these patients was diagnosed according to the Berlin criteria. We defined intubation within 7 days of ARDS diagnosis as ‘early’ intubation and that performed from the eighth day as ‘late’ intubation based on literatures. The outcomes were invasive mechanical ventilation and in-hospital death. The log-binomial regression models were used to explore the risk factors and the Kaplan-Meier statistic was used to estimate the risk of mortality. Results: The median number of days from symptom onset to ARDS diagnosis was 11.0 (IQR, 8.0–13.0). Up to 84.1% COVID-19-related ARDS patients demonstrated multiple organ injuries. The mortality rates were 41.9% and 85.7% in moderate and severe ARDS. The early intubation and the late intubation had the differences in days from symptom onset/hospital admission/ARDS diagnosis to intubation (P = 0.023, P = 0.011, P < 0.001). Compared with the early-intubation group, the late-intubation group showed less severity at admission (median oxygenation index 159.0 95% CI 134.0-203.0 vs. 133.9 95% CI 98.3-183.2), but required more aggressive therapies (ICU 80% vs. 70%, CRRT 50% vs. 10%, prone-position 50% vs. 30%, and ECMO 50% vs. 10%) and had higher risk to die at hospital (RR, 3.18; 95% CI 1.98-5.12). Conclusion: The ARDS caused by COVID-19 was not typical ARDS due to prolonged onset time, multiple organ injuries, and higher mortalities. The late-intubation group showed less severity at admission but higher risk of in-hospital death than the early-intubation group.
Observation of High Dielectric Properties of Mg Substituted BST Ceramic Synthesized by Conventional Solid State Route
Authors: Sharma Anshu , Karol Vidushi , Prakash Chandra 
Published: March 2021

Abstract
Barium Strontium Titanate exhibits ferroelectric or paraelectric behaviour that depends upon the composition and other factors, making it suitable for a number of applications. We have adopted conventional solid state reaction route to synthesize magnesium substituted barium strontium titanate with low concentration of Mg (Mg= 0.005, 0.010 and 0.015). X-ray diffraction has confirmed the perovskite cubic single phase. The dielectric studies were carried out as a function of temperature at different fixed frequencies (1kHz, 10kHz, 50kHz and 100kHz) and as a function of frequency at room temperature. The Curie’s temperature of pure BST was 96°C and for Mg substituted BST samples it dropped from 85°C to 63°C. The dielectric permittivity firstly suppressed and then maximum value observed for Mg content 0.015 and dielectric loss is also less than one. The remanance (Pr) and coercivity (Ec) are obtained and analysed for ceramics samples. Improvement in dielectric properties due to substitution of Magnesium is observed.
Genipin Attenuates Tau Phosphorylation and Aβ Levels in Cellular Models of Alzheimer's Disease
Authors: Li Meiting , Cai Nan , Gu Liang , Yao Lijun , Bi Decheng , Fang Weishan , Lin Zhijian , Wu Yan , Xu Hong , Li Hui , Hu Zhangli , Xu Xu 
Published: March 2021

Abstract
Alzheimer’s disease (AD) is a devastating brain disorder characterized by neurofibrillary tangles and amyloid plaques. Inhibiting Tau protein and amyloid-beta (Aβ) production or removing these molecules are considered potential therapeutic strategies for AD. Genipin is an aglycone and is isolated from the extract of Gardenia jasminoides Ellis fruit. In this study, the effect and molecular mechanisms of genipin on the inhibition of Tau aggregation and Aβ generation were investigated. The results showed that genipin bound to Tau and protected against heparin-induced Tau fibril formation. Moreover, genipin suppressed Tau phosphorylation probably by downregulating the expression of CDK5 and GSK-3β, and activated mTOR-dependent autophagy via the SIRT1/LKB1/AMPK signaling pathway in Tau-overexpressing cells. In addition, genipin decreased Aβ production by inhibiting BACE1 expression through the PERK/eIF2α signaling pathway in N2a/SweAPP cells. These data indicated that genipin could effectively lead to a significant reduction of phosphorylated Tau level and Aβ generation in vitro, suggesting that genipin might be developed into an effective therapeutic complement or a potential nutraceutical for preventing AD.