bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple
Authors: Zhang Qiulei , Xu Chaoran , Wei Haiyang , Fan Wenqi , Li Tianzhong 
Published: October 2021

AbstractAlternaria leaf spot in apple (Malus x domestica), caused by the fungal pathogen Alternaria alternata f. sp. mali (also called A. mali), is a devastating disease resulting in substantial economic losses. We previously established that the resistance (R) protein MdRNL2, containing a coiled-coil, nucleotide-binding, and leucine-rich repeat (CCR-NB-LRR) domain, interacts with another CCR-NB-LRR protein, MdRNL6, to form a MdRNL2–MdRNL6 complex that confers resistance to A. mali. Here, to investigate the function of the MdRNL2–MdRNL6 complex, we identified two novel pathogenesis-related (PR) proteins, MdPR10-1 and MdPR10-2, that interact with MdRNL2. Yeast two-hybrid (Y2H) assays and bimolecular fluorescence complementation (BiFC) assays confirmed that MdPR10-1 and MdPR10-2 interact with MdRNL2 and MdRNL6 at the leucine-rich repeat domain. Transient expression assays demonstrated that accumulation of MdPR10-1 and MdPR10-2 enhanced the resistance of apple to four strains of A. mali that we tested: ALT1, GBYB2, BXSB5, and BXSB7. In vitro antifungal activity assays demonstrated that both the proteins contribute to Alternaria leaf spot resistance by inhibiting fungal growth. Our data provide evidence for a novel regulatory mechanism in which MdRNL2 and MdRNL6 interact with MdPR10-1 and MdPR10-2 to inhibit fungal growth, thereby contributing to Alternaria leaf spot resistance in apple. The identification of these two novel PR proteins will facilitate breeding for fungal disease resistance in apple.
Scientific prizes and the extraordinary growth of scientific topics
Authors: Jin Ching , Ma Yifang , Uzzi Brian 
Published: October 2021

AbstractFast growing scientific topics have famously been key harbingers of the new frontiers of science, yet, large-scale analyses of their genesis and impact are rare. We investigated one possible factor connected with a topic’s extraordinary growth: scientific prizes. Our longitudinal analysis of nearly all recognized prizes worldwide and over 11,000 scientific topics from 19 disciplines indicates that topics associated with a scientific prize experience extraordinary growth in productivity, impact, and new entrants. Relative to matched non-prizewinning topics, prizewinning topics produce 40% more papers and 33% more citations, retain 55% more scientists, and gain 37 and 47% more new entrants and star scientists, respectively, in the first five-to-ten years after the prize. Funding do not account for a prizewinning topic’s growth. Rather, growth is positively related to the degree to which the prize is discipline-specific, conferred for recent research, or has prize money. These findings reveal new dynamics behind scientific innovation and investment.
C. elegans feed yolk to their young in a form of primitive lactation
Authors: Kern Carina C. , Townsend StJohn , Salzmann Antoine , Rendell Nigel B. , Taylor Graham W. , Comisel Ruxandra M. , Foukas Lazaros C. , Bähler Jürg , Gems David 
Published: October 2021

AbstractThe nematode Caenorhabditis elegans exhibits rapid senescence that is promoted by the insulin/IGF-1 signalling (IIS) pathway via regulated processes that are poorly understood. IIS also promotes production of yolk for egg provisioning, which in post-reproductive animals continues in an apparently futile fashion, supported by destructive repurposing of intestinal biomass that contributes to senescence. Here we show that post-reproductive mothers vent yolk which can be consumed by larvae and promotes their growth. This implies that later yolk production is not futile; instead vented yolk functions similarly to milk. Moreover, yolk venting is promoted by IIS. These findings suggest that a self-destructive, lactation-like process effects resource transfer from postreproductive C. elegans mothers to offspring, in a fashion reminiscent of semelparous organisms that reproduce in a single, suicidal burst. That this process is promoted by IIS provides insights into how and why IIS shortens lifespan in C. elegans.
Breaking a dative bond with mechanical forces
Authors: Chen Pengcheng , Fan Dingxin , Zhang Yunlong , Selloni Annabella , Carter Emily A. , Arnold Craig B. , Dankworth David C. , Rucker Steven P. , Chelikowsky James R. , Yao Nan 
Published: October 2021

AbstractBond breaking and forming are essential components of chemical reactions. Recently, the structure and formation of covalent bonds in single molecules have been studied by non-contact atomic force microscopy (AFM). Here, we report the details of a single dative bond breaking process using non-contact AFM. The dative bond between carbon monoxide and ferrous phthalocyanine was ruptured via mechanical forces applied by atomic force microscope tips; the process was quantitatively measured and characterized both experimentally and via quantum-based simulations. Our results show that the bond can be ruptured either by applying an attractive force of ~150 pN or by a repulsive force of ~220 pN with a significant contribution of shear forces, accompanied by changes of the spin state of the system. Our combined experimental and computational studies provide a deeper understanding of the chemical bond breaking process.
Spectrally blue hydrated parent body of asteroid (162173) Ryugu
Authors: Tatsumi Eri , Sakatani Naoya , Riu Lucie , Matsuoka Moe , Honda Rie , Morota Tomokatsu , Kameda Shingo , Nakamura Tomoki , Zolensky Michael , Brunetto Rosario , Hiroi Takahiro , Sasaki Sho , Watanabe Sei’ichiro , Tanaka Satoshi , Takita Jun , Pilorget Cédric , de León Julia , Popescu Marcel , Rizos Juan Luis , Licandro Javier , Palomba Ernesto , Domingue Deborah , Vilas Faith , Campins Humberto , Cho Yuichiro , Yoshioka Kazuo , Sawada Hirotaka , Yokota Yasuhiro , Hayakawa Masahiko , Yamada Manabu , Kouyama Toru , Suzuki Hidehiko , Honda Chikatoshi , Ogawa Kazunori , Kitazato Kohei , Hirata Naru , Hirata Naoyuki , Tsuda Yuichi , Yoshikawa Makoto , Saiki Takanao , Terui Fuyuto , Nakazawa Satoru , Takei Yuto , Takeuchi Hiroshi , Yamamoto Yukio , Okada Tatsuaki , Shimaki Yuri , Shirai Kei , Sugita Seiji 
Published: October 2021

AbstractRyugu is a carbonaceous rubble-pile asteroid visited by the Hayabusa2 spacecraft. Small rubble pile asteroids record the thermal evolution of their much larger parent bodies. However, recent space weathering and/or solar heating create ambiguities between the uppermost layer observable by remote-sensing and the pristine material from the parent body. Hayabusa2 remote-sensing observations find that on the asteroid (162173) Ryugu both north and south pole regions preserve the material least processed by space weathering, which is spectrally blue carbonaceous chondritic material with a 0–3% deep 0.7-µm band absorption, indicative of Fe-bearing phyllosilicates. Here we report that spectrally blue Ryugu’s parent body experienced intensive aqueous alteration and subsequent thermal metamorphism at 570–670 K (300–400 °C), suggesting that Ryugu’s parent body was heated by radioactive decay of short-lived radionuclides possibly because of its early formation 2–2.5 Ma. The samples being brought to Earth by Hayabusa2 will give us our first insights into this epoch in solar system history.
Cytokinin regulates vegetative phase change in Arabidopsis thaliana through the miR172/TOE1-TOE2 module
Authors: Werner Sören , Bartrina Isabel , Schmülling Thomas 
Published: October 2021

AbstractDuring vegetative growth plants pass from a juvenile to an adult phase causing changes in shoot morphology. This vegetative phase change is primarily regulated by the opposite actions of two microRNAs, the inhibitory miR156 and the promoting miR172 as well as their respective target genes, constituting the age pathway. Here we show that the phytohormone cytokinin promotes the juvenile-to-adult phase transition through regulating components of the age pathway. Reduction of cytokinin signalling substantially delayed the transition to the adult stage. tZ-type cytokinin was particularly important as compared to iP- and the inactive cZ-type cytokinin, and root-derived tZ influenced the phase transition significantly. Genetic and transcriptional analyses indicated the requirement of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors and miR172 for cytokinin activity. Two miR172 targets, TARGET OF EAT1 (TOE1) and TOE2 encoding transcriptional repressors were necessary and sufficient to mediate the influence of cytokinin on vegetative phase change. This cytokinin pathway regulating plant aging adds to the complexity of the regulatory network controlling the juvenile-to-adult phase transition and links cytokinin to miRNA action.
Near-infrared to ultra-violet frequency conversion in chalcogenide metasurfaces
Authors: Gao Jiannan , Vincenti Maria Antonietta , Frantz Jesse , Clabeau Anthony , Qiao Xingdu , Feng Liang , Scalora Michael , Litchinitser Natalia M. 
Published: October 2021

AbstractChalcogenide photonics offers unique solutions for a broad range of applications from mid-infrared sensing to integrated, ultrafast, ultrahigh-bandwidth signal processing. However, to date its usage has been limited to the infrared part of the electromagnetic spectrum, thus avoiding ultraviolet and visible ranges due to absorption of chalcogenide glasses. Here, we experimentally demonstrate and report near-infrared to ultraviolet frequency conversion in an As2S3-based metasurface, enabled by a phase locking mechanism between the pump and the inhomogeneous portion of the third harmonic signal. Due to the phase locking, the inhomogeneous component co-propagates with the pump pulse and encounters the same effective dispersion as the infrared pump, and thus experiences little or no absorption, consequently opening previously unexploited spectral range for chalcogenide glass science and applications, despite the presence of strong material absorption in this range.
Development of a therapeutic vaccine targeting Merkel cell polyomavirus capsid protein VP1 against Merkel cell carcinoma
Authors: Xu Dan , Jiang Sheng , He Yue , Jin Xiang , Zhao Gan , Wang Bin 
Published: October 2021

AbstractMerkel cell carcinoma (MCC) is a rare but aggressive skin cancer with a high mortality rate, while Merkel cell polyomavirus (MCV) has been pointed as the causative agent of MCC. A better prognosis of MCC associated with a high level of antibodies against the capsid protein VP1 suggests that anti-VP1 immune response might be essential against MCC growth. In the current study, we developed a VP1-target vaccine formulated with CRA. Using a tumorigenic CMS5-VP1 tumor model, the vaccine-induced a potent antitumor efficacy in a dose-dependent manner was evidently demonstrated and mainly mediated by both VP1-specific CD4+ and CD8+ T-cell responses against the growth of CMS5-VP1 tumors in vaccinated BALB/c mice since the depletion of CD4+ and CD8+ T cells reverse the antitumor effects. Thus, immunotherapy with this vaccine represents a novel approach for the clinical treatment of aggressive MCV-related MCC in humans.
Understanding the role of potential pathways and its components including hypoxia and immune system in case of oral cancer
Authors: Bajrai Leena Hussein , Sohrab Sayed Sartaj , Mobashir Mohammad , Kamal Mohammad Amjad , Rizvi Moshahid Alam , Azhar Esam Ibraheem 
Published: October 2021

AbstractThere are a few biological functions or phenomenon which are universally associated with majority of the cancers and hypoxia and immune systems are among them. Hypoxia often occurs in most of the cancers which helps the cells in adapting different responses with respect to the normal cells which may be the activation of signaling pathways which regulate proliferation, angiogenesis, and cell death. Similar to it, immune signaling pathways are known to play critical roles in cancers. Moreover, there are a number of genes which are known to be associated with these hypoxia and immune system and appear to direct affect the tumor growth and propagations. Cancer is among the leading cause of death and oral cancer is the tenth-leading cause due to cancer death. In this study, we were mainly interested to understand the impact of alteration in the expression of hypoxia and immune system-related genes and their contribution to head and neck squamous cell carcinoma. Moreover, we have collected the genes associated with hypoxia and immune system from the literatures. In this work, we have performed meta-analysis of the gene and microRNA expression and mutational datasets obtained from public database for different grades of tumor in case of oral cancer. Based on our results, we conclude that the critical pathways which dominantly enriched are associated with metabolism, cell cycle, immune system and based on the survival analysis of the hypoxic genes, we observe that the potential genes associated with head and neck squamous cell carcinoma and its progression are STC2, PGK1, P4HA1, HK1, SPIB, ANXA5, SERPINE1, HGF, PFKM, TGFB1, L1CAM, ELK4, EHF, and CDK2.
Real-time tracking of ionic nano-domains under shear flow
Authors: Cafolla Clodomiro , Voïtchovsky Kislon 
Published: October 2021

AbstractThe behaviour of ions at solid–liquid interfaces underpins countless phenomena, from the conduction of nervous impulses to charge transfer in solar cells. In most cases, ions do not operate as isolated entities, but in conjunction with neighbouring ions and the surrounding solution. In aqueous solutions, recent studies suggest the existence of group dynamics through water-mediated clusters but results allowing direct tracking of ionic domains with atomic precision are scarce. Here, we use high-speed atomic force microscopy to track the evolution of Rb+, K+, Na+ and Ca2+ nano-domains containing 20 to 120 ions adsorbed at the surface of mica in aqueous solution. The interface is exposed to a shear flow able to influence the lateral motion of single ions and clusters. The results show that, when in groups, metal ions tend to move with a relatively slow dynamics, as can be expected from a correlated group motion, with an average residence timescale of ~ 1–2 s for individual ions at a given atomic site. The average group velocity of the clusters depends on the ions’ charge density and can be explained by the ion’s hydration state. The lateral shear flow of the fluid is insufficient to desorb ions, but indirectly influences the diffusion dynamics by acting on ions in close vicinity to the surface. The results provide insights into the dynamics of ion clusters when adsorbed onto an immersed solid under shear flow.
L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
Authors: Mindus Claire , van Staaveren Nienke , Fuchs Dietmar , Gostner Johanna M. , Kjaer Joergen B. , Kunze Wolfgang , Mian M. Firoz , Shoveller Anna K. , Forsythe Paul , Harlander-Matauschek Alexandra 
Published: October 2021

AbstractIn mammals, early-life probiotic supplementation is a promising tool for preventing unfavourable, gut microbiome-related behavioural, immunological, and aromatic amino acid alterations later in life. In laying hens, feather-pecking behaviour is proposed to be a consequence of gut-brain axis dysregulation. Lactobacillus rhamnosus decreases stress-induced severe feather pecking in adult hens, but whether its effect in pullets is more robust is unknown. Consequently, we investigated whether early-life, oral supplementation with a single Lactobacillus rhamnosus strain can prevent stress-induced feather-pecking behaviour in chickens. To this end, we monitored both the short- and long-term effects of the probiotic supplement on behaviour and related physiological parameters. We hypothesized that L. rhamnosus would reduce pecking behaviour by modulating the biological pathways associated with this detrimental behaviour, namely aromatic amino acid turnover linked to neurotransmitter production and stress-related immune responses. We report that stress decreased the proportion of cytotoxic T cells in the tonsils (P = 0.047). Counteracting this T cell depression, birds receiving the L. rhamnosus supplementation significantly increased all T lymphocyte subset proportions (P < 0.05). Both phenotypic and genotypic feather peckers had lower plasma tryptophan concentrations compared to their non-pecking counterparts. The probiotic supplement caused a short-term increase in plasma tryptophan (P < 0.001) and the TRP:(PHE + TYR) ratio (P < 0.001). The administration of stressors did not significantly increase feather pecking in pullets, an observation consistent with the age-dependent onset of pecking behaviour. Despite minimal changes to behaviour, our data demonstrate the impact of L. rhamnosus supplementation on the immune system and the turnover of the serotonin precursor tryptophan. Our findings indicate that L. rhamnosus exerts a transient, beneficial effect on the immune response and tryptophan catabolism in pullets.
Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
Authors: Sofi Irshad Ahmad , Rashid Irfan , Lone Javaid Yousuf , Tyagi Sandhya , Reshi Zafar A. , Mir Reyazul Rouf 
Published: October 2021

AbstractHabitat loss due to climate change may cause the extinction of the clonal species with a limited distribution range. Thus, determining the genetic diversity required for adaptability by these species in sensitive ecosystems can help infer the chances of their survival and spread in changing climate. We studied the genetic diversity and population structure of Sambucus wightiana—a clonal endemic plant species of the Himalayan region for understanding its possible survival chances in anticipated climate change. Eight polymorphic microsatellite markers were used to study the allelic/genetic diversity and population structure. In addition, ITS1–ITS4 Sanger sequencing was used for phylogeny and SNP detection. A total number of 73 alleles were scored for 37 genotypes at 17 loci for 8 SSRs markers. The population structural analysis using the SSR marker data led to identifying two sub-populations in our collection of 37 S. wightiana genotypes, with 11 genotypes having mixed ancestry. The ITS sequence data show a specific allele in higher frequency in a particular sub-population, indicating variation in different S. wightiana accessions at the sequence level. The genotypic data of SSR markers and trait data of 11 traits of S. wightiana, when analyzed together, revealed five significant Marker-Trait Associations (MTAs) through Single Marker Analysis (SMA) or regression analysis. Most of the SSR markers were found to be associated with more than one trait, indicating the usefulness of these markers for working out marker-trait associations. Moderate to high genetic diversity observed in the present study may provide insurance against climate change to S. wightiana and help its further spread.
Using electrocochleography to detect sensory and neural damages in a gerbil model
Authors: Meenderink Sebastiaan W. F. , Lin Xiaohui , Dong Wei 
Published: October 2021

AbstractHearing is one of the five sensory organs that allows us to interact with society and our environment. However, one in eight Americans suffers from sensorineural hearing loss that is great enough to adversely impact their daily life. There is an urgent need to identify what part/degree of the auditory pathway (sensory or neural) is compromised so that appropriate treatment/intervention can be implemented. Single- or two-tone evoked potentials, the electrocochleography (eCochG), were measured along the auditory pathway, i.e., at the round window and remotely at the vertex, with simultaneous recordings of ear canal distortion product otoacoustic emissions. Sensory (cochlear) and neural components in the (remote-) eCochG responses showed distinct level- and frequency-dependent features allowing to be differentiated from each other. Specifically, the distortion products in the (remote-)eCochGs can precisely localize the sensory damage showing that they are effective to determine the sensory or neural damage along the auditory pathway.
Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management
Authors: Ahmad Parvaiz , Raja Vaseem , Ashraf Muhammed , Wijaya Leonard , Bajguz Andrzej , Alyemeni Mohammed Nasser 
Published: October 2021

AbstractCadmium stress is one of the chief environmental cues that can substantially reduce plant growth. In the present research, we studied the effect of jasmonic acid (JA) and gibberellic acid (GA3) applied individually and/or in combination to chickpea (Cicer arietinum) plants exposed to 150 µM cadmium sulphate. Cadmium stress resulted in reduced plant growth and pigment contents. Moreover, chickpea plants under cadmium contamination displayed higher levels of electrolytic leakage, H2O2,and malonaldehyde, as well as lower relative water content. Plants primed with JA (1 nM) and those foliar-fed with GA3(10–6 M) showed improved metal tolerance by reducing the accumulation of reactive oxygen species, malonaldehyde and electrolytic leakage, and increasing relative water content. . Osmoprotectants like proline and glycinebetaine increased under cadmium contamination. Additionally, the enzymatic activities and non-enzymatic antioxidant levels increased markedly under Cd stress, but application of JA as well as of GA3further improved these attributes. Enzymes pertaining to the ascorbate glutathione and glyoxylase systems increased significantly when the chickpea plants were exposed to Cd. However, JA and GA3applied singly or in combination showed improved enzymatic activities as well as nutrient uptake, whereas they reduced the metal accumulation in chickpea plants. Taken together, our findings demonstrated that JA and GA3are suitable agents for regulating Cd stress resistance in chickpea plants.
Change sign detection with differential MDL change statistics and its applications to COVID-19 pandemic analysis
Authors: Yamanishi Kenji , Xu Linchuan , Yuki Ryo , Fukushima Shintaro , Lin Chuan-hao 
Published: October 2021

AbstractWe are concerned with the issue of detecting changes and their signs from a data stream. For example, when given time series of COVID-19 cases in a region, we may raise early warning signals of an epidemic by detecting signs of changes in the data. We propose a novel methodology to address this issue. The key idea is to employ a new information-theoretic notion, which we call the differential minimum description length change statistics (D-MDL), for measuring the scores of change sign. We first give a fundamental theory for D-MDL. We then demonstrate its effectiveness using synthetic datasets. We apply it to detecting early warning signals of the COVID-19 epidemic using time series of the cases for individual countries. We empirically demonstrate that D-MDL is able to raise early warning signals of events such as significant increase/decrease of cases. Remarkably, for about $$64\%$$

64
%

of the events of significant increase of cases in studied countries, our method can detect warning signals as early as nearly six days on average before the events, buying considerably long time for making responses. We further relate the warning signals to the dynamics of the basic reproduction number R0 and the timing of social distancing. The results show that our method is a promising approach to the epidemic analysis from a data science viewpoint.