bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment outcomes of pediatric acute myeloid leukemia: a retrospective analysis from 1996 to 2019 in Taiwan
Authors: Yang Yung-Li , Jaing Tang-Her , Chen Shih-Hsiang , Liu Hsi-Che , Hung Iou-Jih , Lin Dong-Tsamn , Yang Chao-Ping , Peng Ching-Tien , Lin Kai-Hsin , Hsiao Chih-Cheng , Jou Shiann-Tarng , Chen Jiann-Shiuh , Lin Ming-Tsan , Wang Shih-Chung , Chang Te-Kau , Huang Fang-Liang , Cheng Chao-Neng , Wu Kang-Hsi , Sheen Jiunn-Ming , Chen Shu-Huey , Lu Meng-Yao , Hung Giun-Yi , Yen Hsiu-Ju , Hsieh Yuh-Lin , Wang Jinn-Li , Chang Yu-Hsiang , Chang Hsiu-Hao , Yeh Ting-Chi , Weng Te-Fu , Hou Jen-Yin , Chen Bow-Wen , Chen Rong-Long , Wang Lin-Yen , Ho Wan-Ling , Chen Yu-Chieh , Cheng Shin-Nan , Chao Yu-Hua , Yang Shang-Hsien , Huang Ting-Huan , Chou Shu-Wei , Lin Chien-Yu , Chen Hsuan-Yu , Chao Yu-Mei Y. , Liang Der-Cherng , Chang Tai-Tsung 
Published: March 2021

AbstractImprovement in outcomes of children with acute myeloid leukemia (AML) is attributed to several refinements in clinical management. We evaluated treatment outcomes of Taiwanese pediatric AML patients in the past 20 years. Overall, 860 de novo AML patients aged 0–18 years and registered in the Childhood Cancer Foundation of R.O.C during January 1996–December 2019 were included. Survival analysis was performed to identify factors that improved treatment outcomes. Regardless of treatment modalities used, patients during 2008–2019 had better 5-year event-free survival (EFS) and overall survival (OS) rates than patients during 1996–2007. For patients received the TPOG-AML-97A treatment, only 5-year OS rates were significantly different between patients diagnosed before and after 2008. Patients with RUNX1–RUNX1T1 had similar relapse-free survival rates, but 5-year OS rates were better during 2008–2019. However, the survival of patients who received hematopoietic stem-cell transplantations (HSCT) did not differ significantly before and after 2008. For patients without relapse, the 5-year OS improved during 2008–2019. Non-relapse mortality decreased annually, and cumulative relapse rates were similar. In conclusion, 5-year EFS and OS rates improved during 2008–2019, though intensities of chemotherapy treatments were similar before and after 2008. Non-relapse mortality decreased gradually. Further treatment strategies including more intensive chemotherapy, novel agents’ use, identification of high-risk patients using genotyping and minimal residual disease, early intervention of HSCT, and antibiotic prophylaxis can be considered for future clinical protocol designs in Taiwan.
Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
Authors: Wang Kai , Yan Tian-ze , Xu Shi-long , Yan Xu , Zhou Qun-feng , Zhao Xin-hui , Li Yan-feng , Wu Zhong-xiu , Qin Peng , Fu Chen-jian , Fu Jun , Zhou Yan-biao , Yang Yuan-zhu 
Published: March 2021

AbstractCadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1jap haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1jap haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 jap and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7jap haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety.
Effects of home quarantine during COVID-19 lockdown on physical activity and dietary habits of adults in Saudi Arabia
Authors: Alfawaz Hanan , Amer Osama E. , Aljumah Abdulaziz A. , Aldisi Dara A. , Enani Mushira A. , Aljohani Naji J. , Alotaibi Naif H. , Alshingetti Naemah , Alomar Suliman Y. , Khattak Malak Nawaz Khan , Sabico Shaun , Al-Daghri Nasser M. 
Published: March 2021

AbstractPublic health endorsements during the present COVID-19 pandemic has led the governments of largely affected countries to imply policies that restrict social mobility to slow COVID-19 spread. The study aimed to explore the effects of COVID-19 home quarantine on lifestyle and health behavior of Saudi residents. An online survey in Saudi Arabia was launched from May 11 to June 6, 2020. The survey was designed by multidisciplinary scientists and academics uploaded and shared through the Google platform in Arabic and English languages. Questions presented related to responses “before” and “during” COVID-19 home quarantine. A total of 1965 respondents participated and were included in the analysis [921 (47.0%) males and 1044 (53.0%) females]. Non-Saudis were more likely to increase their physical activity during quarantine [odds ratio (95% confidence interval 1.41 (1.11–1.79); p < 0.005]. Prevalence of participants walking daily for more than 4 times per week significantly decreased during pandemic (before vs during, 30.5% vs 29.1%) which was in parallel to the significant increase in the prevalence of participants who did not perform daily walking during the quarantine (21% vs 22.9%; p < 0.001). The prevalence of participants who often consume snacks between meals increased during quarantine (27.4% vs 29.4%, p < 0.001), while the prevalence of participants who never consumed fresh fruits and vegetables significantly increased during home quarantine (2.4% vs 3.7%; p = 0.019). The lockdown imposed in Saudi Arabia modestly but significantly impacted physical activity and dietary behaviors of several citizens and residents in an unhealthy way. Interventions to alleviate these acute adverse lifestyle behaviors during pandemic should be formulated.
All-trans retinoic acid and protein kinase C α/β1 inhibitor combined treatment targets cancer stem cells and impairs breast tumor progression
Authors: Berardi Damian Emilio , Ariza Bareño Lizeth , Amigo Natalia , Cañonero Luciana , Pelagatti Maria de las Nieves , Motter Andrea Nora , Taruselli María Agustina , Díaz Bessone María Inés , Cirigliano Stefano Martin , Edelstein Alexis , Peters María Giselle , Diament Miriam , Urtreger Alejandro Jorge , Todaro Laura Beatriz 
Published: March 2021

AbstractBreast cancer is the leading cause of cancer death among women worldwide. Blocking a single signaling pathway is often an ineffective therapy, especially in the case of aggressive or drug-resistant tumors. Since we have previously described the mechanism involved in the crosstalk between Retinoic Acid system and protein kinase C (PKC) pathway, the rationale of our study was to evaluate the effect of combining all-trans-retinoic acid (ATRA) with a classical PCK inhibitor (Gö6976) in preclinical settings. Employing hormone-independent mammary cancer models, Gö6976 and ATRA combined treatment induced a synergistic reduction in proliferative potential that correlated with an increased apoptosis and RARs modulation towards an anti-oncogenic profile. Combined treatment also impairs growth, self-renewal and clonogenicity potential of cancer stem cells and reduced tumor growth, metastatic spread and cancer stem cells frequency in vivo. An in-silico analysis of “Kaplan–Meier plotter” database indicated that low PKCα together with high RARα mRNA expression is a favorable prognosis factor for hormone-independent breast cancer patients. Here we demonstrate that a classical PKC inhibitor potentiates ATRA antitumor effects also targeting cancer stem cells growth, self-renewal and frequency.
Monitoring geological storage of CO2: a new approach
Authors: Fawad Manzar , Mondol Nazmul Haque 
Published: March 2021

AbstractGeological CO2 storage can be employed to reduce greenhouse gas emissions to the atmosphere. Depleted oil and gas reservoirs, deep saline aquifers, and coal beds are considered to be viable subsurface CO2 storage options. Remote monitoring is essential for observing CO2 plume migration and potential leak detection during and after injection. Leak detection is probably the main risk, though overall monitoring for the plume boundaries and verification of stored volumes are also necessary. There are many effective remote CO2 monitoring techniques with various benefits and limitations. We suggest a new approach using a combination of repeated seismic and electromagnetic surveys to delineate CO2 plume and estimate the gas saturation in a saline reservoir during the lifetime of a storage site. This study deals with the CO2 plume delineation and saturation estimation using a combination of seismic and electromagnetic or controlled-source electromagnetic (EM/CSEM) synthetic data. We assumed two scenarios over a period of 40 years; Case 1 was modeled assuming both seismic and EM repeated surveys were acquired, whereas, in Case 2, repeated EM surveys were taken with only before injection (baseline) 3D seismic data available. Our results show that monitoring the CO2 plume in terms of extent and saturation is possible both by (i) using a repeated seismic and electromagnetic, and (ii) using a baseline seismic in combination with repeated electromagnetic data. Due to the nature of the seismic and EM techniques, spatial coverage from the reservoir's base to the surface makes it possible to detect the CO2 plume’s lateral and vertical migration. However, the CSEM low resolution and depth uncertainties are some limitations that need consideration. These results also have implications for monitoring oil production—especially with water flooding, hydrocarbon exploration, and freshwater aquifer identification.
Effects of mating on reproductive performance of Coccophagus japonicus Compere (Hymenoptera: Aphelinidae)
Authors: Li Xian , Shen Shunzhang , Fu Yueguan , Chen Junyu , Li Lei , Han Dongyin , Zhu Junhong , Zhang Fangping 
Published: March 2021

AbstractCoccophagus japonicus Compere, an endoparasitoid of Parasaissetia nigra Nietner, has great potential for biological control. To assess the influence of mating on the reproductive performance of this parasitoid, we examined the effects of mating on ovarian development, female longevity and number of eggs laid. The results showed that the egg volume in the ovary of C. japonicus first increased and then decreased with increases in the age of female adults. The peak egg volume in the ovary of mated females occurred 2 days earlier than that of virgin females. Within the female age range of 0–15 days, the numbers of eggs at stages I, II, and III first increased and then decreased with increases in the age of female C. japonicus, whereas the number of eggs at stage IV increased. The duration of the coexistence of females and males significantly influenced the length and width of the female ovaries, and the longest ovary tube and the highest number of eggs were obtained with a coexistence duration of 0 days. C. japonicus female longevity decreased with increases in the number of matings, and the number of eggs laid by females within 15 days decreased with increasing delays in mating. In conclusion, mating can shorten the longevity of C. japonicus females, and selecting newly emerged virgin females for mating can significantly improve the number of eggs laid and the breeding efficiency of the parasitoid.
Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
Authors: Azimian-Zavareh Vajihe , Dehghani-Ghobadi Zeinab , Ebrahimi Marzieh , Mirzazadeh Kian , Nazarenko Irina , Hossein Ghamartaj 
Published: March 2021

AbstractWnt5A signals through various receptors that confer versatile biological functions. Here, we used Wnt5A overexpressing human ovarian SKOV-3 and OVCAR-3 stable clones for assessing integrin expression, cell proliferation, migration, invasion, and the ability of multicellular aggregates (MCAs) formation. We found here, that Wnt5A regulates differently the expression of its receptors in the stable Wnt5A overexpressing clones. The expression levels of Frizzled (FZD)-2 and -5, were increased in different clones. However ROR-1, -2 expression levels were differently regulated in clones. Wnt5A overexpressing clones showed increased cell proliferation, migration, and clonogenicity. Moreover, Wnt5A overexpressing SKOV-3 clone showed increased MCAs formation ability. Cell invasion had been increased in OVCAR-3-derived clones, while this was decreased in SKOV-3-derived clone. Importantly, αv integrin expression levels were increased in all assessed clones, accompanied by increased cell attachment to fibronectin and focal adhesion kinase activity. Moreover, the treatment of clones with Box5 as a Wnt5A/FZD5 antagonist abrogates ITGAV increase, cell proliferation, migration, and their attachment to fibronectin. Accordingly, we observed significantly higher expression levels of ITGAV and ITGB3 in human high-grade serous ovarian cancer specimens and ITGAV correlated positively with Wnt5A in metastatic serous type ovarian cancer. In summary, we hypothesize here, that Wnt5A/FZD-5 signaling modulate αv integrin expression levels that could be associated with ovarian cancer cell proliferation, migration, and fibronectin attachment.
A Raman algorithm to estimate human age from protein structural variations in autopsy skin samples: a protein biological clock
Authors: Miyamori Daisuke , Uemura Takeshi , Zhu Wenliang , Fujikawa Kei , Nakaya Takaaki , Teramukai Satoshi , Pezzotti Giuseppe , Ikegaya Hiroshi 
Published: March 2021

AbstractThe recent increase of the number of unidentified cadavers has become a serious problem throughout the world. As a simple and objective method for age estimation, we attempted to utilize Raman spectrometry for forensic identification. Raman spectroscopy is an optical-based vibrational spectroscopic technique that provides detailed information regarding a sample’s molecular composition and structures. Building upon our previous proof-of-concept study, we measured the Raman spectra of abdominal skin samples from 132 autopsy cases and the protein-folding intensity ratio, RPF, defined as the ratio between the Raman signals from a random coil an α-helix. There was a strong negative correlation between age and RPF with a Pearson correlation coefficient of r = 0.878. Four models, based on linear (RPF), squared (RPF2), sex, and RPF by sex interaction terms, were examined. The results of cross validation suggested that the second model including linear and squared terms was the best model with the lowest root mean squared error (11.3 years of age) and the highest coefficient of determination (0.743). Our results indicate that the there was a high correlation between the age and RPF and the Raman biological clock of protein folding can be used as a simple and objective forensic age estimation method for unidentified cadavers.
Effect of depressive symptom and depressive disorder on glaucoma incidence in elderly
Authors: Jung Younhea , Han Kyungdo , Wang Sheng-min , Yoon Hye yeon , Moon Jung Il 
Published: March 2021

AbstractAlthough depression and glaucoma share several common pathophysiology, the risk of glaucoma in patients with depression has not been reported. Thus, we investigated the effect of depressive symptom and depressive disorder on glaucoma incidence. In this nationwide population-based cohort study, all subjects receiving the National Screening Program at the age of 66 during 2009–2014 were included. These subjects were divided into depression group and no depression group based on subjective depressive symptoms and clinically diagnosed depressive disorder and were tracked until 2017 for development of glaucoma. Of the 922,769 subjects included in the study, 191,636 (20.77%) subjects were categorized as depression group. Subjects with depression showed increased hazard of developing glaucoma (adjusted HR = 1.12[95% confidence interval (CI), 1.09–1.15]) than those without depression. The risk of glaucoma increased sequentially from those with no depression to those with subjective depressive symptom (adjusted HR = 1.09[95% CI, 1.06–1.13]), those with clinically diagnosed depressive disorder (adjusted HR = 1.23[95% CI, 1.14–1.32]), and those with both subjective depressive symptom and clinically diagnosed depressive disorder (adjusted HR = 1.36[95% CI, 1.22–1.52]). Our analyses suggest that individuals with depression had a greater risk of developing glaucoma than those without depression. Subjective depressive symptoms and clinically diagnosed depressive disorder independently and synergistically increased the risk of glaucoma incidence.
Liver stiffness and insulin resistance in predicting recurrence for early stage hepatoma patients after curative resection
Authors: Wang Jing-Houng , Li Wei-Feng , Yong Chee-Chien , Liu Yueh-Wei , Lu Sheng-Nan , Wang Chih-Chi 
Published: March 2021

AbstractCurative resection is recommended for patient with early stage hepatocellular carcinoma (HCC), however, the prognosis is limited by high recurrence rate. This study was to investigate liver stiffness (LS) and metabolic factor in prediction of HCC recurrence for patients with early stage HCC who had undergone curative resection. Consecutive patients with suspicion of HCC who had undergone curative resection were prospectively enrolled. Transient elastography was performed to determine LS pre-operatively. The demographics, clinical characteristics and histological findings were recorded. All patients were followed up regularly until recurrence, death or last visit. Ninety-four patients with early stage HCC were enrolled. LS positively correlated with fibrosis stage (r = 0.666). In a median follow-up of 3.2 years, forty patients developed recurrences including 22 recurrences after 1-year post resection. The 5-year cumulative recurrence rate was 44.2%. LS was the independent factor associated with recurrence. Patients with LS > 8.5 kPa had higher 5-year cumulative recurrence rate (59.8% vs 25.1%, p = 0.007). For the prediction of recurrence after 1-year post resection, LS > 8.5 kPa (hazard ratio 2.72) and homeostatic model assessment for insulin resistance index (HOMA-IR) (hazard ratio 1.24) were independent factors in multivariate analysis. Those patients with both LS > 8.5 kPa and HOMA-IR > 2.3 had the highest recurrence rate after 1-year post resection.
The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis
Authors: Arablou Tahereh , Aryaeian Naheed , Khodaverdi Sepideh , Kolahdouz-Mohammadi Roya , Moradi Zahra , Rashidi Nesa , Delbandi Ali-Akbar 
Published: March 2021

AbstractResveratrol is a phytochemical with anti-angiogenic, anti-inflammatory, and antioxidant properties. The present study has evaluated the effect of resveratrol on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloproteinase-9 (MMP-9) as factors related to endometriosis progression. Thirteen eutopic (EuESCs) and 8 ectopic (EESCs) endometrial stromal cells from women with endometriosis and 11 control endometrial stromal cells (CESCs) were treated with resveratrol (100 µM) for 6, 24 and 48 h. The gene and protein expression levels of VEGF, TGF-β, and MMP-9 were measured using real-time PCR and ELISA methods, respectively. Results showed that the basal gene and protein expression of VEGF and MMP-9 were higher in EESCs compared to EuESCs and CESCs (P < 0.01 to  < 0.001 and P < 0.05 to  < 0.01 respectively). Also, resveratrol treatment decreased the gene and protein expression of VEGF and MMP-9 in EuESCs, EESCs and CESCs (P < 0.05 to  < 0.01 and P < 0.05 to  < 0.01 respectively) and gene and protein expression of TGF-β in EESCs and EuESCs (P < 0.05 to  < 0.01). The effect of resveratrol in reduction of VEGF gene expression was statistically more noticeable in EESCs compared to EuESCs and CESCs (P < 0.05). According to the findings, resveratrol may ameliorate endometriosis progression through reducing the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells (ESCs).
Gender differences of clinical and polysomnographic findings with obstructive sleep apnea syndrome
Authors: Zhou Xiaobo , Zhou Bo , Li Zhe , Lu Qiao , Li Shaoping , Pu Zhongyin , Luo Fang 
Published: March 2021

AbstractObstructive sleep apnea syndrome (OSAS) is underdiagnosed in females and gender differences in clinical and polysomnographic findings have not been widely investigated in China. We examined clinical and polysomnographic differences between males and females with OSAS in order to determine the influence of gender on clinical presentation and polysomnographic features. Data were collected from 303 adult patients diagnosed with OSAS (237 males and 66 females) from 2017 to 2019. All the patients completed physical examination, Epworth sleepiness scale, and whole night polysomnography. AVONA, univariate and multivariate logistic regression analyses were conducted to assess gender differences of clinical and polysomnographic findings with OSAS. P < 0.05 was statistically significant. The average age was 48.4 ± 12.6 years for females and 43.4 ± 12.4 years for males. Compared with female patients with OSAS, male patients were taller and heavier, had higher systolic blood pressure in the morning, shorter duration of slow wave sleep, more micro-arousal events, greater AHI, and more complex sleep apnea events. There are obvious gender differences of clinical and polysomnographic characteristics with OSAS. Understanding gender differences will contribute to better clinical recognition of OSAS in females as well as the provision of proper health care and therapeutic practice.
Type 2 diabetes progression differently affects endothelial function and vascular contractility in the aorta and the pulmonary artery
Authors: Melo Bernardete F. , Prieto-Lloret Jesus , Cabral Marlene D. , Martins Fatima O. , Martins Inês B. , Sacramento Joana F. , Ruivo Pedro , Carvalho Tânia , Conde Silvia V. 
Published: March 2021

AbstractType 2 diabetes (T2D) is associated with cardiovascular and pulmonary disease. How T2D affects pulmonary endothelial function is not well characterized. We investigated the effects of T2D progression on contractility machinery and endothelial function in the pulmonary and systemic circulation and the mechanisms promoting the dysfunction, using pulmonary artery (PA) and aorta. A high-fat (HF, 3 weeks 60% lipid-rich diet) and a high-fat/high-sucrose (HFHSu, combined 60% lipid-rich diet and 35% sucrose during 25 weeks) groups were used as prediabetes and T2D rat models. We found that T2D progression differently affects endothelial function and vascular contractility in the aorta and PA, with the contractile machinery being altered in the PA and aorta in prediabetes and T2D animals; and endothelial function being affected in both models in the aorta but only affected in the PA of T2D animals, meaning that PA is more resistant than aorta to endothelial dysfunction. Additionally, PA and systemic endothelial dysfunction in diabetic rats were associated with alterations in the nitrergic system and inflammatory pathways. PA dysfunction in T2D involves endothelial wall mineralization. The understanding of the mechanisms behind PA dysfunction in T2D can lead to significant advances in both preventative and therapeutic treatments of pulmonary disease-associated diabetes.