bluuebunny/crossref_metadata_2025_split huggingface dataset:
Harmful behaviour through plasmid transfer: a successful evolutionary strategy of bacteria harbouring conjugative plasmids
Authors: Domingues Célia P. F. , Rebelo João S. , Monteiro Francisca , Nogueira Teresa , Dionisio Francisco 
Published: November 2021

Conjugative plasmids are extrachromosomal mobile genetic elements pervasive among bacteria. Plasmids' acquisition often lowers cells' growth rate, so their ubiquity has been a matter of debate. Chromosomes occasionally mutate, rendering plasmids cost-free. However, these compensatory mutations typically take hundreds of generations to appear after plasmid arrival. By then, it could be too late to compete with fast-growing plasmid-free cells successfully. Moreover, arriving plasmids would have to wait hundreds of generations for compensatory mutations to appear in the chromosome of their new host. We hypothesize that plasmid-donor cells may use the plasmid as a ‘weapon’ to compete with plasmid-free cells, particularly in structured environments. Cells already adapted to plasmids may increase their inclusive fitness through plasmid transfer to impose a cost to nearby plasmid-free cells and increase the replication opportunities of nearby relatives. A mathematical model suggests conditions under which the proposed hypothesis works, and computer simulations tested the long-term plasmid maintenance. Our hypothesis explains the maintenance of conjugative plasmids not coding for beneficial genes.This article is part of the theme issue ‘The secret lives of microbial mobile genetic elements’.
Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring
Authors: Smith Caroline J. , Lintz Tania , Clark Madeline J. , Malacon Karen E. , Constantino Nicholas J. , Kim Veronica J. , Jo Young C. , Alonso-Caraballo Yanaira , Abiad Alia , Bilbo Staci D. , Chartoff Elena H. 
Published: November 2021

AbstractThe current opioid epidemic has dramatically increased the number of children who are prenatally exposed to opioids, including oxycodone. A number of social and cognitive abnormalities have been documented in these children as they reach young adulthood. However, little is known about the mechanisms underlying developmental effects of prenatal opioid exposure. Microglia, the resident immune cells of the brain, respond to acute opioid exposure in adulthood. Moreover, microglia are known to sculpt neural circuits during healthy development. Indeed, we recently found that microglial phagocytosis of dopamine D1 receptors (D1R) in the nucleus accumbens (NAc) is required for the natural developmental decline in NAc-D1R that occurs between adolescence and adulthood in rats. This microglial pruning occurs only in males, and is required for the normal developmental trajectory of social play behavior. However, virtually nothing is known as to whether this developmental program is altered by prenatal exposure to opioids. Here, we show in rats that maternal oxycodone self-administration during pregnancy leads to reduced adolescent microglial phagocytosis of D1R and subsequently higher D1R density within the NAc in adult male, but not female, offspring. Finally, we show that prenatal opioid exposure abolishes the extinction of oxycodone-conditioned place preference in these male offspring. This work demonstrates for the first time that microglia play a key role in translating prenatal opioid exposure to long-term changes in neural systems and behavior.HighlightsPrenatal opioid exposure decreases offspring viability and body weight in males and femalesPrenatal opioid exposure decreases microglial phagocytosis of D1R in the nucleus accumbens in males onlyPrenatal opioid exposure increases nucleus accumbens dopamine D1 receptor expression in males but not femalesAdult males fail to extinguish oxycodone-conditioned place preference following prenatal oxycodone exposure
A facile platform to engineer E. coli tyrosyl-tRNA synthetase adds new chemistries to the eukaryotic genetic code, including a phosphotyrosine mimic
Authors: Grasso Katherine T. , Roy Soumya Jyoti Singha , Yeo Megan Jin Rae , Soni Chintan , Osgood Arianna O. , Hillenbrand Christen M. , Chatterjee Abhishek 
Published: November 2021

AbstractThe E. coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNAEcTyr pair offers an attractive platform to genetically encode new noncanonical amino acids (ncAA) in eukaryotes. However, challenges associated with a eukaryotic selection system, which is needed for its engineering, has impeded its success in the past. Recently, we showed that EcTyrRS can be engineered using a facile E. coli based selection system, in a strain where the endogenous tyrosyl pair has been substituted with an archaeal counterpart. However, a significant cross-reactivity between the UAG-suppressing tRNACUAEcTyr and the bacterial glutaminyl-tRNA synthetase limited the scope of this strategy, preventing the selection of moderately active EcTyrRS mutants. Here we report an engineered tRNACUAEcTyr that overcomes this cross-reactivity. Optimized selection systems using this tRNA enabled efficient enrichment of both strongly and weakly active ncAA-selective EcTyrRS mutants. We also developed a wide-dynamic range (WiDR) antibiotic selection to further enhance the activities of the weaker first-generation EcTyrRS mutants. We demonstrated the utility of our platform by developing several new EcTyrRS mutants that efficiently incorporate useful ncAAs in mammalian cells, including photo-affinity probes, bioconjugation handles, and a non-hydrolyzable mimic of phosphotyrosine.
X chromosomes show relaxed selection and complete somatic dosage compensation acrossTimemastick insect species
Authors: Parker Darren J. , Jaron Kamil S. , Dumas Zoé , Robinson-Rechavi Marc , Schwander Tanja 
Published: November 2021

AbstractSex chromosomes have evolved repeatedly across the tree of life. As they are present in different copy numbers in males and females, they are expected to experience different selection pressures than the autosomes, with consequences including a faster rate of evolution, increased accumulation of sexually antagonistic alleles, and the evolution of dosage compensation. Whether these consequences are general or linked to idiosyncrasies of specific taxa is not clear as relatively few taxa have been studied thus far. Here we use wholegenome sequencing to identify and characterize the evolution of the X chromosome in five species ofTimemastick insects with XX:X0 sex determination. The X chromosome had a similar size (approximately 11% of the genome) and gene content across all five species, suggesting that the X chromosome originated prior to the diversification of the genus. Genes on the X showed evidence of relaxed selection (elevated dN/dS) and a slower evolutionary rate (dN + dS) than genes on the autosomes, likely due to sex-biased mutation rates. Genes on the X also showed almost complete dosage compensation in somatic tissues (heads and legs), but dosage compensation was absent in the reproductive tracts. Contrary to prediction, sex-biased genes showed little enrichment on the X, suggesting that the advantage X-linkage provides to the accumulation of sexually antagonistic alleles is weak. Overall, we found the consequences of X-linkage on gene sequences and expression to be similar acrossTimemaspecies, showing the characteristics of the X chromosome are surprisingly consistent over 30 million years of evolution.
Deciphering the impact of responsiveness on customer satisfaction, cross-buying behaviour, revisit intention and referral behaviour
Authors: Sharma Deepika , Paul Justin , Dhir Sanjay , Taggar Rashi 
Published: November 2021

PurposeThe ease and convenience of online shopping are shifting the customers to e-tailers. This has prompted offline retailers to re-examine behavioural patterns along with a reconfiguration for a responsive retail model. The paper investigates the influence of responsiveness on customer satisfaction, cross-buying behaviour, revisit intention and referral behaviour.Design/methodology/approachData were collected via a survey answered by 793 fashion customers from India, and for data analysis, partial least square structural equation modelling (PLS-SEM) was employed. Path analysis was used to determine the interrelationships amongst the constructs used in the study.FindingsThe standardized path coefficients depict competitive responsiveness as the highest contributor of retailers' responsiveness followed by service responsiveness, employee responsiveness and customer responsiveness. The findings suggest that customer satisfaction acts as the biggest contributor to referral behaviour followed by cross-buying behaviour and revisit intentions.Originality/valueThis study has made a substantial contribution to fashion apparel retailing. The findings revealed that responsive retailing influences the customers' post-purchase behaviour as they engage in more cross-buying, revisiting and referral behaviour. The retailers are encouraged to carefully monitor their preparedness to deliver a combination of sensory, emotional, cognitive and social experience to their customers.
Three-dimensional data-driven magnetostatic field computation using real-world measurement data
Authors: Galetzka Armin , Loukrezis Dimitrios , De Gersem Herbert 
Published: November 2021

Purpose
The purpose of this paper is to present the applicability of data-driven solvers to computationally demanding three-dimensional problems and their practical usability when using real-world measurement data.

Design/methodology/approach
Instead of using a hard-coded phenomenological material model within the solver, the data-driven computing approach reformulates the boundary value problem such that the field solution is directly computed on raw measurement data. The data-driven formulation results in a double minimization problem based on Lagrange multipliers, where the sought solution must conform to Maxwell’s equations while at the same time being as close as possible to the available measurement data. The data-driven solver is applied to a three-dimensional model of a direct current electromagnet.

Findings
Numerical results for data sets of increasing cardinality verify that the data-driven solver recovers the conventional solution. Additionally, the practical usability of the solver is shown by using real-world measurement data. This work concludes that the data-driven magnetostatic finite element solver is applicable to computationally demanding three-dimensional problems, as well as in cases where a prescribed material model is not available.

Originality/value
Although the mathematical derivation of the data-driven problem is well presented in the referenced papers, the application to computationally demanding real-world problems, including real measurement data and its rigorous discussion, is missing. The presented work closes this gap and shows the applicability of data-driven solvers to challenging, real-world test cases.
TPTPC and BHC integrated grid connected energy storage system for power loss reduction
Authors: Krishnan Suresh , Pandi Pothuraju , Mopidevi Subbarao 
Published: November 2021

Purpose
This paper aims to propose a bidirectional hidden converter (BHC)-based three-phase DC–AC conversion for energy storage application. BHC is the new concept to vary an energy storage device voltage into wide range. Hidden converter power loss and power rating are reduced by using zero-sequence injection-based carrier-based pulse-width modulation (CBPWM) strategy.

Design/methodology/approach
By using this control strategy, a BHC processes only little amount of power during double-stage conversion, mostly during direct or single-stage conversion of the three-phase three-port converter (TPTPC) only processing the maximum power.

Findings
TPTPC consists of two sets of positive group switches for inversion process, one set of switches is regular inverter switches called vertical positive group switches, and the second set is anti-series switches, which are horizontally connected for direct or single-stage conversion.

Originality/value
Characteristics, principles and implementations of proposed DC–AC 3Ø conversion system and its PWM strategy are analyzed. Through experimental outputs, the effectiveness and viability of the proposed solutions are validated.
Illegitimate tasks and occupational outcomes: the impact of vertical collectivism
Authors: Akyurek Server Sevil , Can Ozge 
Published: November 2021

PurposeThis study aims to understand essential work and occupational consequences of employees’ illegitimate task (ILT) experiences (unreasonable and unnecessary task demands) under the influence of vertical collectivist (VC) values.Design/methodology/approachData were collected via a survey from 503 teachers in the Turkish public education sector. The hypotheses were tested using structural equation modeling.FindingsFindings of this study reveal that unnecessary tasks decrease employees’ professional identification and perceived occupational prestige, whereas unreasonable tasks weaken their workplace well-being. Results also show that employees with higher VC orientation feel these adverse effects to a lesser extent.Research limitations/implicationsThis study demonstrates that individual-level cultural values play a significant role in understanding task-related dynamics and consequences at the workplace. It brings new theoretical insights to job design and work stress literature regarding what similar factors can mitigate task pressures on employees.Practical implicationsA key practical insight from the findings is that human resources management experts should create a positive task environment where ILT demands are not welcome by analyzing jobs and skill requirements in detail, communicating task decisions regularly with employees and providing them with the necessary work support.Social implicationsUnderstanding the impact of ILT can greatly help to assess the quality of the education system and the value of teaching occupation in society.Originality/valueILT have been mainly discussed without considering the effect of different cultural orientations. This is the first study empirically showing the diverse effects of two ILT dimensions on essential occupational outcomes in connection to individual-level cultural influences.
A bottom-up role of information asymmetry: opening the black-box of firms’ resource allocation mechanism
Authors: Blouch Riffat , Khan Muhammad Majid , Shakeel Wajid 
Published: November 2021

PurposeDrawing on the concept of resource-based theory of the firm; the purpose of this study is to analyze the influence of firms’ strategic approaches on the firm performance via indirect effect using a multilevel, bottom-up approach.Design/methodology/approachUsing the survey method, the present study obtains data from 104 diversified manufacturing firms and analyzes the bottom-up effect of firms’ strategic approach on efficient resource allocation using Mplus.FindingsGiven the prevailing conditions, the study found that the motive of most firms is growth rather than risk mitigation or collaboration in the manufacturing sector of Pakistan. Furthermore, the study found that the bottom-level employees’ information asymmetry has a significant impact on the strategic resource allocation decision, which can lead to resource allocation inefficiency.Research limitations/implicationsDespite making a unique contribution, the present study has few limitations requiring researchers’ attention to in the forthcoming. These include a low amount of data, self-reporting technique and failure to include all the possible reason that could cause resource allocation inefficiency.Practical implicationsThe present research has potential applications for managers of the manufacturing industry. First, the study alerts managers about the challenges of resource allocation. At the same time, this study provides critical implication for managing bottom-level employees.Originality/valueThe current study has made a sizable impression in the literature of resource-based theory of the firm by recommending a model that augments the theoretical foundation of strategic management of the firm. So, closely considering these insights would be helping for the firms for allocating resources efficiently in the manufacturing industry.
The effects of on-the-job embeddedness and its sub-dimensions on small-sized hotel employees’ organizational commitment, work engagement and turnover intentions
Authors: Ampofo Emmanuel Twumasi , Karatepe Osman M. 
Published: November 2021

PurposeThis paper aims to examine the impacts of links, fit and sacrifice as the sub-dimensions of on-the-job embeddedness (JE) on turnover intentions through the mediating roles of affective organizational commitment (AOC) and work engagement (WENG) and explore AOC and WENG as the two mediators linking on-the-JE to quitting intentions.Design/methodology/approachData collected from small-sized hotels in three waves in Ghana were used to assess the aforesaid relationships. The hypothesized relationships were tested using structural equation modeling.FindingsThe findings reveal that on-the-JE is negatively related to turnover intentions. Additionally, the relationship between on-the-JE and turnover intentions is partly mediated by AOC and WENG. The findings further demonstrate that AOC and WENG partly mediate the impact of links on turnover intentions.Practical implicationsManagement of hotels should enhance formal and informal connections between employees and their coworkers and supervisors through socialization tactics. Establishing and maintaining an environment that fosters social support would increase employees’ sense of belongingness and their proclivity to remain with the organization.Originality/valueLittle is known about the effects of the sub-dimensions of on-the-JE on work-related consequences in the hospitality and tourism literature. The literature is also devoid of evidence concerning affective variables as the potential mediators relating the sub-dimensions of on-the JE to turnover intentions. The study sets out to fill in these gaps.
Assessment of the causes and effects of design deficiencies for large construction projects using social network analysis
Authors: Shoar Shahab , Chileshe Nicholas , Payan Shamsi 
Published: November 2021

PurposeThe purpose of this study is to investigate the latent interrelationships of causes and effects of design deficiencies (DDs) and to identify the most crucial ones by considering the interactions among them.Design/methodology/approachFirst, through a comprehensive literature review, the most critical causes of DDs were identified. The review eventuated in a list of 22 causes and 12 effects, which were categorized into six groups. Second, through the rules of system dynamics and the interactions between the causes and effects were modeled and illustrated using causal loop diagrams (CLDs). With the aid of semi-structured interviews with 20 competent experts, the resultant CLDs were also validated. Third, the opinions of 54 experts, who were chosen from the Iranian community of clients and consultants, were solicited concerning the degree of influence which each factor (causes or effects) exerts on others. Finally, the social network analysis (SNA) approach was deployed to analyze and prioritize factors based on the gathered data from experts.FindingsSNA results indicated that factors such as “design firms' staff rework” and “design firms' loss of reputation” are the most central factors affecting DDs. The model results also identified that factors such as “schedule variance”, “workload” and “lack of quality control and supervision during the design phase” have the highest overall impact on DDs. In the end, some recommendations to address major factors and links were also put forward. Overall, more communications between the pair of stakeholder groups and continuous learning from project experiences are believed to be the main strategies.Originality/valueIt is believed that this study has provided a comprehensive understanding of causal mechanisms among factors, which can assist project managers of different parties (clients, contractors and consulting firms) in taking more effective actions to ameliorate the quality of design documents.
Exploring contributions of drones towards Industry 4.0
Authors: Javaid Mohd , Khan Ibrahim Haleem , Singh Ravi Pratap , Rab Shanay , Suman Rajiv 
Published: November 2021

PurposeUnmanned aerial vehicles are commonly known as UAVs and drones. Nowadays, industries have begun to realise the operational and economic benefits of drone-enabled tasks. The Internet of Things (IoT), Big Data, drones, etc., represent implementable advanced technologies intended to accomplish Industry 4.0. The purpose of this study is to discuss the significant contributions of drones for Industry 4.0.Design/methodology/approachNowadays, drones are used for inspections, mapping and surveying in difficult or hazardous locations. For writing this paper, relevant research papers on drone for Industry 4.0 are identified from various research platforms such as Scopus, Google Scholar, ResearchGate and ScienceDirect. Given the enormous extent of the topic, this work analyses many papers, reports and news stories in an attempt to comprehend and clarify Industry 4.0.FindingsDrones are being implemented in manufacturing, entertainment industries (cinematography, etc.) and machinery across the world. Thermal-imaging devices attached to drones can detect variable heat levels emanating from a facility, trigger the sprinkler system and inform emergency authorities. Due partly to their utility and adaptability in industrial areas such as energy, transportation, engineering and more, autonomous drones significantly impact Industry 4.0. This paper discusses drones and their types. Several technological advances and primary extents of drones for Industry 4.0 are diagrammatically elaborated. Further, the authors identified and discussed 19 major applications of drones for Industry 4.0.Originality/valueThis paper’s originality lies in its discussion and exploration of the capabilities of drones for Industry 4.0, especially in manufacturing organisations. In addition to improving efficiency and site productivity, drones can easily undertake routine inspections and check streamlines operations and maintenance procedures. This work contributes to creating a common foundation for comprehending Industry 4.0 outcomes from many disciplinary viewpoints, allowing for more research and development for industrial innovation and technological progress.