bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineered Porous/Hollow Burkholderia Pseudomallei Loading Tumor Lysate as a Vaccine
Authors: Huang Feng-Ying , Dai Shu-Zhen , Wang Jin-Yan , Lin Ying-Ying , Wang Cai-Chun , Zheng Wu-Ping , Tan Guang-hong 
Published: June 2021

Abstract
Due to its size, shape, and inherent expression of pathogen-associated molecular patterns and invasion-assistant adhesion proteins, Burkholderia pseudomallei can easily attach to, and then be internalized by, dendritic cells (DCs), leading to more efficient antigen cross-presentation if modified as carrier. Herein, we engineered Burkholderia pseudomallei as a porous/hollow carrier (SB) for loading tumor lysates (L) and CpG (C) to be used as a tumor vaccine (SB-LC). We found that the adhesion proteins of Burkholderia pseudomallei promote internalization of the SB-LC vaccine by DCs, and result in enhanced DC maturation and antigen cross-presentation. SB-LC induces robust cellular and humoral antitumor responses that synergistically inhibit tumor growth with minimal adverse side effects in several tumor models. Moreover, SB-LC vaccination reverses the immunosuppressive tumor microenvironment, apparently as a result of CD8+-induced tumor ferroptosis. Thus, SB-LC is a potential model tumor vaccine for translating into a clinically viable treatment option.
Decelerate of Amyloid Fibrillation By The Alkaloids Extracted From Stephania Venosa
Authors: Daowtak Krai , Pompimon Wilart , Salerno Milena , Udomtanakunchai Chatchanok 
Published: June 2021

Abstract
Naturally occurring phytochemical compounds have received considerable attention as alternative candidates for anti-amyloidogenic agents. This study, utilizing human insulin and amyloid beta peptide as an in vitro model, determined the anti-amyloid effects of alkaloids extracted derived from Stephania venosa. Alkaloids extracts including crebanine, O-methylbulbocapnine, tetrahydropalmatine and N-methyltetrahydropalmatine were used. Inhibition of amyloid protein aggregation were studied by fluorescence spectroscopy. Most alkaloids, except N-methyltetrahydropalmatine, exhibited the inhibitory properties against amyloid fibrillation either insulin or amyloid-beta peptide. Among alkaloids group, crebanine and tetrahydropalmatine showed the potent properties of anti-amyloidogenesis. These results suggest that alkaloids could be used as a natural compound for the development of drugs against amyloid protein aggregation for treatment of amyloid-related diseases.
Even-Chain UFA Pattern, n-3 Long-Chain PUFA Pattern and Risk of Esophageal Squamous Cell Carcinoma in Southeast of China
Authors: Hu Chanchan , Lin Zheng , Liu Zhiqiang , Tang Xuwei , Song Jianyu , Lin Jianbo , Chen Yuanmei , Hu Zhijian 
Published: June 2021

Abstract
Objective: To characterize and examine associations between dietary fatty acid intake patterns and the risk of esophageal squamous cell carcinoma (ESCC).Methods: A total of 422 patients and 423 controls were recruited. Dietary fatty acids, as a percentage of total fat, were entered into a factor analysis. Multinomial logistic regression and restricted cubic spline were used to evaluate the risk of ESCC specific for different dietary fatty acid patterns (FAPs). A forest plot was applied to show the potential effect modification.Results: The factor analysis generated 4 major fatty acid patterns: a long-chain SFA (LC-SFA) pattern; an even-chain unsaturated fatty acid (EC-UFA) pattern; a short and medium-chain SFA (SMC-SFA) pattern, and an n-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) pattern. In the multivariate-adjusted model, the odds ratios (ORs) with 95% confidence interval (CI) of ESCC were 2.069(1.314,3.257), and 0.525 (0.340–0.811) for the highest versus the lowest tertile of EC-UFA pattern and n-3 LC-PUFA pattern, respectively. The LC-SFA, SMC-SFA patterns were not associated with ESCC. There existed a nonlinear positive association between the EC-UFA pattern and the risk of ESCC (p for nonlinearity <0.05), nevertheless, there was a nonlinear negative association between the n-3 LC-PUFA pattern and the risk of ESCC (p for nonlinearity <0.001). Multiplicative interaction between fried food, pickled food, hard food, tobacco smoking, alcohol drinking, and four FAPs was also observed.Conclusions: Our study indicates that EC-UFA pattern, n-3 LC-PUFA pattern intake are associated with ESCC, which might provide a potential dietary intervention for ESCC prevention.
The Prognostic Significance of MAFK and its Methylation in Cervical Cancer
Authors: Zhang Mengjun , Li Hao , Liu Yuan , Hou Siyu , Cui Ping , Sun Zhenxing , Yue Yin , Chen Xiuwei 
Published: June 2021

Abstract
Background: The purpose of this study was to determine the value of MAFK as a biomarker of cervical cancer prognosis and to explore its methylation and possible cellular signaling pathways. Methods: We analyzed the cervical cancer data of The Cancer Genome Atlas (TCGA) through bioinformatics, including MAFK expression, methylation, prognosis and genome enrichment analysis. Results: MAFK expression was higher in cervical cancer tissues and was negatively correlated with the methylation levels of five CpG sites. MAFK is an independent prognostic factor of cervical cancer and is involved in the Nod-like receptor signaling pathway. CMap analysis screened four drug candidates for cervical cancer treatment. Conclusions: We confirmed that MAFK is a novel prognostic biomarker for cervical cancer and aberrant methylation may also affect MAFK expression and carcinogenesis. This study provides a new molecular target for the prognostic evaluation and treatment of cervical cancer.
Highly Accurate, Specific And Sensitive Quantitation By Droplet Digital PCR Reveals The Stability of Intrahepatic Hepatitis B Virus cccDNA
Authors: Hayashi Sanae , Isogawa Masanori , Kawashima Keigo , Ito Kyoko , Chuaypen Natthaya , Morine Yuji , Shimada Mitsuo , Higashi-Kuwata Nobuyo , Tangkijvanich Pisit , Mitsuya Hiroaki , Tanaka Yasuhito 
Published: June 2021

Abstract
The persistence of covalently closed circular DNA (cccDNA) poses a major obstacle to curing chronic hepatitis B (CHB). Here, we used droplet digital PCR (ddPCR) for cccDNA quantitation. ddPCR measured a less than two-fold difference in the intrahepatic cccDNA content more accurately than conventional real-time PCR (qPCR), (R2=0.9416 and R2=0.8963, respectively) and had also higher sensitivity and specificity than qPCR. The results of ddPCR correlated more closely with serum HB core-related antigen (R2=0.9843) than HB surface antigen (HBsAg) (R2=0.9742) in 24 HBV-infected human-liver-chimeric mice (PXB-mice). We demonstrated that the total cccDNA content did not change during liver repopulation, although the cccDNA content per hepatocyte was reduced in PXB-mice after entecavir treatment. In the 6 patients with HBV-related hepatocellular carcinoma, ddPCR detected cccDNA in both tumor and non-tumor tissues. In 13 HBeAg-negative CHB patients with pegylated interferon alpha-2a, cccDNA contents from paired biopsies were more significantly reduced in virological response (VR) than in non-VR at week 48 (p=0.0051). Interestingly, cccDNA levels were the lowest in VR with HBsAg clearance but remained detectable after the treatment. Collectively, ddPCR revealed that cccDNA content is stable during hepatocyte proliferation and persists at quantifiable levels, even after serum HBsAg clearance.
Study on the Causes of Secondary Cracks of the Eave Wall Mural of Daxiong Hall at Fengguo Temple in Yixian, Liaoning, China
Authors: Liu Cheng , He Yuan , Li Qian , Wang Fei 
Published: June 2021

Abstract
Built in Kaitai nine years of Liao Dynasty (1020 A.D.), the Daxiong Hall of Fengguo Temple in Yixian County, Liaoning Province, is one of the largest existing ancient single-eave wooden structure in China. There are nearly 470m 2 murals of Yuan Dynasty on the four walls of the hall. Since the in-situ reinforcement and protection of the mural were carried out in the 1980s in cooperation with the maintenance and restoration project of the main hall, the seriously developed cracks have become the primary factor affecting the structural stability of the mural. In order to find out the macroscopic causes of secondary cracks, endoscope, infrared thermal imager, three-dimensional laser scanner and other equipment were applied to study the relations between cracks’ attitude and structural defects of the eave wall, as well as the pathway that external environment act on the mural noumenon through comprehensive investigation, and the idea of restoring the stability of the mural by blocking the air channel and offsetting the environmental stress is put forward.
Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation
Authors: Ruiz David , Santibáñez Miguel , Alio-Lavín Bernardo , Álvarez Isabel , Berja Ana , Montalbán Coral , Vázquez Luis Alberto 
Published: June 2021

Abstract
Aims: To study the evolution of mitochondrial-derived peptides (MDPs) and their relationship with changes in insulin sensitivity from the early stages of pregnancy.Methods: MDPs (humanin and MOTSc) were assessed in the first and second trimesters of gestation in 28 pregnant women with gestational diabetes mellitus (GDM) and a subgroup of 45 matched pregnant women without GDM. Insulin resistance (IR) was defined as a HOMA-IR index ≥70 percentile.Results: We observed a significant reduction in both humanin and MOTSc levels from the first to the second trimesters of pregnancy. After adjusting for BMI we did not find an association between low levels of humanin and the occurrence of a high HOMA-IR index (adjusted OR= 2.63 and 3.14, linear p-trend 0.260 and 0.175, respectively). Regarding MOTSc, an association was found only for the second trimester: adjusted OR=7.68 (95% CI 1.49-39.67), linear p-trend=0.012. No significant associations were observed in humanin change with insulin resistance throughout pregnancy, but changes in MOTSc levels were significantly associated with HOMA-IR index: adjusted OR 3.73 (95% CI 1.03-13.50).Conclusions: MOTSc levels, especially a strong decrease from the first to the second trimester of gestation, may be involved in increasing insulin resistance during early gestation.CLINICAL TRIAL REGISTRATIONNot applicable
Discordant American Society of Anesthesiologists Physical Status Scoring between Anesthesiologists and Surgeons Is Correlated With Adverse Patient Outcomes: A Retrospective Cohort Study of 46284 Elective Surgical Patients
Authors: Kwa Charlene Xian Wen , Cui Jiaqian , Lim Daniel Yan Zheng , Sim Yilin Eileen , Ke Yuhe , abdullah hairil Rizal 
Published: June 2021

Abstract
BackgroundThe American Society of Anesthesiologists Physical Status Classification (ASA) score is used for communication of patient health status, risk scoring, benchmarking and financial claims. Prior studies using hypothetical scenarios have shown poor concordance of ASA scoring among healthcare providers. However, there is a paucity of concordance studies using real-world data, as well as studies of clinical factors or patient outcomes associated with discordant scoring. The study aims to assess real-world ASA score concordance between surgeons and anesthesiologists, factors surrounding discordance and its impact on patient outcomes. MethodsThis retrospective cohort study was conducted in a tertiary academic medical center on 46284 consecutive patients undergoing elective surgery between January 2017 and December 2019. ASA scores entered by surgeons and anesthesiologists, patient demographics, and post-operative outcomes were collected. We assessed the concordance of preoperative ASA scoring between surgeons and anesthesiologists, clinical factors associated with score discordance, the impact of score discordance on clinically important outcomes, and the discriminative ability of the two scores for 30-day mortality, 1-year mortality, and intensive care unit (ICU) admission. Statistical tests used included Cohen’s weighted 𝜅 score, chi-square test, t-test, unadjusted odds ratios and logistic regression models. ResultsThe ASA score showed moderate concordance (weighted Cohen’s 𝜅 0.53) between surgeons and anesthesiologists. 15098 patients (32.6%) had discordant scores, of which 11985 (79.4%) were scored lower by surgeons. We found significant associations between discordant scores and anesthesiologist-assessed comorbidities, patient age and race. Patients with discordant scores had a higher risk of 30-day mortality (odds ratio 2.00, 95% confidence interval [CI] = 1.52-2.62, p<0.0001), 1-year mortality (odds ratio 1.53, 95% CI = 1.38-1.69, p < 0.0001), and ICU admission >24 hours (odds ratio 1.69, 95% CI = 1.47-1.94, p< 0.0001), and stratified analyses showed a trend towards higher risk when the surgeons’ ASA score was lower. Conclusions﻿There is moderate concordance between surgeons and anesthesiologists in assigning the ASA classification. Discordant ASA scores are associated with adverse patient outcomes. Hence, there is a need for improved standardization of ASA scoring and cross-specialty review in ASA-discordant cases.
Fecundity, Survival and Susceptibility of Bedbugs (Cimex Hemipterus), Exposed to Selected Classes of Insecticides: A Short Report
Authors: OBOH MARY Aigbiremo , Olusegun-Joseph Taiye Shade , Awoniyi Adedayo Michael , Ileaboya Maureen Ihinosen , Lawal Bukola Tawakalitu , Fagbohun Ifeoluwa Kayode 
Published: June 2021

Abstract
Background: Tropical bed bug, Cimex hemipterus, is a nocturnal obligate blood-sucking ecto-parasite of humans that is highly prevalent in Nigeria. Bed bug control heavily relies on the application of several insecticides-based formulations. Insecticide resistance in bed bug populations has been widely reported. Here, we assessed the mortality and fecundity of bed bugs following exposure to different classes of insecticides. Methods: Bed bugs were collected from students’ halls of resident and exposed to DDT (4%), permethrin (0.75), bendiocarb (0.1) and malathion (4%) insecticide-impregnated papers. Mortality and number of eggs laid by each exposed groups were recorded at 24hrs, 48hrs and 72hrs. Results: The relative numbers of eggs produced by bedbugs in the DDT, bendiocarb and malathion assays were lower than what was observed in the control 24 hours after exposure. The highest reduction in egg production was observed in the malathion exposed-group compared to the other insecticides and the control group. The impact of the different assays on bugs mortality revealed DDT to produce a time-dependent outcome with the highest death rate (36.7%) recorded 24 hours’ post-exposure. In contrast, malathion and bendiocarb impacted the most mortality (53.3% and 46.6%) after 72 hours. Conclusion: This study revealed an occurrence of suspected insecticide resistance to all classes of insecticide used in bed bug populations in Lagos State, Nigeria.
High-dose Salvage Re-irradiation in Recurrent/progressive Adult Diffuse High-Grade Glioma: Development of a Novel Prognostic Scoring System
Authors: Gupta Tejpal , Maitre Madan , Maitre Priyamvada , Chatterjee Abhishek , Dasgupta Archya , Moiyadi Aliasgar , Shetty Prakash , Epari Sridhar , Sahay Ayushi , Patil Vijay , Goda Jayant Sastri , Jalali Rakesh 
Published: June 2021

Abstract
Purpose: Over the past two decades, high-dose salvage re-irradiation (re-RT) has been used increasingly in the multimodality management of adults with recurrent/progressive high-grade glioma (HGG). Several factors that determine outcomes following re-RT have been incorporated into prognostic models to guide patient selection. We aimed to develop a novel four-tiered prognostic model incorporating relevant molecular markers from our single-institutional cohort of patients treated with high-dose salvage re-RT for recurrent/progressive HGG. Methods: Various patient, disease, and treatment-related factors impacting upon survival following salvage re-RT were identified through univariate analysis. Each of these prognostic factors was further subdivided and assigned scores of 0 (good-risk), 1 (intermediate-risk), or 2 (high-risk) to create a composite prognostic scoring system. Scores from individual prognostic factors were added to derive the cumulative score (ranging from 0 to 16), with increasing scores indicating worsening prognosis. Results: A total of 111 patients with recurrent/progressive HGG treated with salvage high-dose re-RT constituted the study dataset. We could assign patients into four prognostic subgroups (A=15 patients, score 0-3); (B=50 patients, score 4-7); (C=33 patients, score 8-10); and (D=13 patients, score 11-16) with completely non-overlapping survival curves suggesting the good discriminatory ability of the proposed prognostic scoring system. Post-re-RT survival was significantly higher in Group A compared to groups B, C, and D, respectively (stratified log-rank p-value <0.0001). Conclusion: A novel four-tiered prognostic scoring system incorporating traditional factors as well as relevant molecular markers is proposed for selecting patients appropriately for high-dose salvage re-RT.
Embedded Obfuscated Barcodes for Identification of Genuine Additive Manufactured Parts
Authors: Chen Fei , PINISETTY DINESH , Gupta Nikhil 
Published: June 2021

Abstract
Additive manufacturing (AM) has been adopted for manufacturing complex shaped highly customized components for aerospace, automotive, and medical fields, where intellectual property protection and counterfeit detection are major concerns. New technologies such as Blockchain have been promising in supply chain authentication. However, AM due to layer-by-layer manufacturing process provides opportunities of embedding information inside the part during manufacturing, which has been explored recently to embed identification codes inside the parts. The present work studies the possibility of printing a barcode inside the additively manufactured part and develops a scheme to obfuscate the code design to read differently from different directions to enhance the security and protect the intellectual property. The embedded three-dimensional codes are scanned using a micro-CT scan. This scheme of embedded obfuscated codes proves to be a highly customizable and efficient process while securing product design files.
Research on Resonance Mechanism and Collaborative Optimization of Double-cavity Self-Excited Oscillating Pulse Cavitation Jet Nozzle
Authors: yuan xiaoming , Wang Li , Wang Weidong , Zhang Lijie , Zhu Yong 
Published: June 2021

Abstract
The peak value and pulsation amplitude of the self-excited oscillating pulse cavitation jet nozzle are important indexes to evaluate the jet performance. It is of great significance in theory and engineering practice to predict the peak value of the self-excited oscillating pulse cavitation jet nozzle accurately. In order to investigate the evolution mechanism of the inner and outer flow field of a double-cavity self-excited oscillation pulse cavitation jet nozzle, a simulation model of the jet impact test of the nozzle was established. Before entrance rounded corners, former cavity cavity diameter, cavity cavity length, before the cavity under the nozzle diameter, cavity, the cavity cavity after entry the rounded, lumen diameter, cavity length and cavity after cavity under the nozzle diameter as design variables, and strike force to combat force peak pulse amplitude as the target variable, the orthogonal experiment method, back propagation neural network combined with non dominated sorting genetic algorithm, The collaborative optimization design method of self-excited oscillating pulse cavitation jet nozzle was determined. Based on the collaborative optimization results, the 3D printing technology was used to manufacture the visualization test model of the flow field of the self-excited oscillating pulse cavitation jet nozzle, and the experimental verification was carried out. The results show that when the inlet pressure is 2MPa, the main and secondary order of the influences of various factors on the jet performance of the nozzle is the nozzle diameter under the front cavity, the diameter of the back cavity, the diameter of the front cavity, the length of the front cavity, the nozzle diameter under the back cavity, the cavity distance, the fillet of the back cavity, the fillet of the front cavity and the length of the back cavity. Compared with the optimal result of orthogonal test, the amplitude of impact pulsation and the peak value of impact force are increased by 14.61% and 2.42% respectively. The optimal structure of the nozzle determined by collaborative optimization can produce obvious pulse cavitation jet, and the cavitation region of the nozzle cavity contracts periodically with time. The higher the inlet pressure, the higher the cavitation intensity and the higher the content of hollow bubble. This study can promote the development of jet performance calculation of self-excited oscillation pulse cavitation jet nozzles, and provide support for the design of self-excited oscillation pulse cavitation jet nozzles.
Synthesis of Layered Octadecyltrimethylammonium-Templated Aluminosilicate and its Use as a Thickening Agentfor Lubricating Grease
Authors: Han Lukun , Niu Wenxing , Zhao Xinrui , Xu Hong , Dong Jinxiang 
Published: June 2021

Abstract
We describe herein the use of octadecyltrimethylammonium-templated aluminosilicate (designated as LS) as a thickener to induce gelation. LS samples with different aluminum/silicon molar ratios (Al/Si = 0, 0.05, 0.10, 0.15, 0.20) were synthesized hydrothermally and characterized by X-ray diffraction analysis, 27Al MAS NMR spectra, elemental analysis, and scanning electron microscopy. The aluminum/silicon molar ratio was shown to be an important factor affecting the rheological properties of LS gels. With increasing Al/Si molar ratio, the viscoelasticity and structural strength of LS gel were enhanced, the dropping point increased, and the amount of oil separation decreased. LS(0.20) gel exhibited superior relative elastic character. The strength of the LS(0.20) gel was also enhanced with increasing LS(0.20) content. In SRV tests, LS(0.20) gel with different contents showed good performance in terms of load-bearing ability and anti-wear property, indicating that LS was strongly adhered on the friction surface, and thereby promoted lubrication. Owing to simple preparation, the promising rheological and tribological properties, LS gel hold great potential application in lubricating grease.
A preliminary study on the efficacy and safety of 3-hole VS 5-hole laparoscopic radical cystectomy
Authors: peng longfei , bi liangkuan , yu dexin , wang jinyou 
Published: June 2021

Abstract
Objective

To investigate the efficacy and safety of three hole laparoscopic radical cystectomy(LRC) in comparison with five hole LRC.
Methods

The patients who underwent radical cystectomy from January 2019 to March 2021 in our hospital were retrospectively analyzed.Basic information and preoperative and postoperative data were collected(such as gender, age, hemoglobin, creatinine, operation time, blood loss, ventilation time, etc).Statistical analysis was used to determine whether there was statistical significance between the two groups(3-hole VS 5-hole).
Results

A total of 99 patients with radical cystectomy and urinary diversion were collected.Three hole laparoscopy included 33 cases and five hole laparoscopy included 66 cases.The basic characteristics of the two groups of data did not have significant statistical significance.The operation time and exsufflation time of 3-hole method were significantly shorter than that of 5-hole method in patients with ileal conduit. In patients with orthotopic neobladder, the exsufflation time and postoperative hospital stay of 3-hole method were significantly shorter than that of 5-hole method.Total laparoscopic surgery has shorter ventilation time than in vitro urinary diversion.
Conclusions

Compared with 5-hole LRC, 3-hole LRC can reduce the operation time, shorten the hospitalization time, reduce the hospitalization cost and save medical resources. However, due to the small sample size and short follow-up time, the long-term benefits need to be further observed.