bluuebunny/crossref_metadata_2025_split huggingface dataset:
Business-Government Relations in the EU
Authors: Coen David , Katsaitis Alexander , Vannoni Matia 
Published: March 2021

Abstract
This monograph charts and assesses business lobbying in Brussels, taking into consideration its nuances. Indeed, a number of disciplines, theories, and methodological approaches grapple with this subject. The EU is one of the largest trading blocs and lobbying environments in the world. It is also a unique socio-political arena, in constant flux over the last fifty years. Business has not been idle; expanding its reach outside of the national domain, since the 1970s it has developed a spectacular advocacy toolbox, particularly in Europe and the US. Government affairs offices in Brussels today hardly resemble those forty years ago in quality or quantity. The relationship, is multi-layered, multi-level, multi-actor, and cuts across a series of institutional, thematic, legal, social, and governmental networks. With this in mind, this monograph draws on two different disciplines that act as a theoretical prism: political science, and business and management studies. The former helps make sense of the demand side of the equation, the role of government in shaping business activity. The latter provides the supply side, the company’s inner workings and its strategic choice to mobilize and lobby the EU. We employ three analytic perspectives: (i) macro; (ii) meso; (iii) micro. Bringing together different perspectives to business-government affairs in the EU, we aim to provide a rounded theoretical understanding of the relationship, a rich set of conceptual tools for its examination, and a detailed empirical mapping.
The Nature of the Policy Field and Business-Government Relations
Authors: Coen David , Katsaitis Alexander , Vannoni Matia 
Published: March 2021

Abstract
This chapter studies the nature of the policy field and its impact on business-lobbying in the EU. Drawing from theories on institutional legitimacy and information-access it observes the variation in density and diversity across regulatory and (re)distributive policy fields, and committee hearings. Empirically, the chapter employs business group surveys, conducts meticulous analysis of a population of 12,000 interest groups lobbying preferences across multiple policy fields assessing their density and diversity as well as their clustering. It includes a case study on business diverse coalition building focusing on net neutrality. Finally, the chapter observes the distribution of business interests across the European Parliament’s committee hearings. In doing so, this chapter contributes to discussions on legitimacy and public policy, chameleon pluralism, business mobilization, and the nature of the policy good.
Plant-specific histone deacetylases are essential for early and late stages of Medicago nodule development
Authors: Li Huchen , Schilderink Stefan , Cao Qingqin , Kulikova Olga , Bisseling Ton 
Published: March 2021

Abstract
Legume and rhizobium species can establish a nitrogen-fixing nodule symbiosis. Previous studies have shown that several transcription factors that play a role in (lateral) root development are also involved in nodule development. Chromatin remodeling factors, like transcription factors, are key players in regulating gene expression. However, studies have not investigated whether chromatin remodeling genes that are essential for root development are also involved in nodule development. Here, we studied the role of Medicago (Medicago truncatula) histone deacetylases (MtHDTs) in nodule development. Arabidopsis (Arabidopsis thaliana) orthologs of HDTs have been shown to play a role in root development. MtHDT expression is induced in nodule primordia and is maintained in the nodule meristem and infection zone. Conditional, nodule-specific knockdown of MtHDT expression by RNAi blocks nodule primordium development. A few nodules may still form, but their nodule meristems are smaller, and rhizobial colonization of the cells derived from the meristem is markedly reduced. Although the HDTs are expressed during nodule and root development, transcriptome analyses indicate that HDTs control the development of each organ in a different manner. During nodule development, the MtHDTs positively regulate 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 (MtHMGR1). Decreased expression of MtHMGR1 is sufficient to explain the inhibition of primordium formation.
A positive feedback loop mediated by CsERF31 initiates female cucumber flower development
Authors: Pan Jian , Wen Haifan , Chen Guanqun , Lin Wen-Hui , Du Hui , Chen Yue , Zhang Leyu , Lian Hongli , Wang Gang , Cai Run , Pan Junsong 
Published: March 2021

Abstract
Sex determination is a crucially important developmental event that is pervasive throughout nature and enhances the adaptation of species. Among plants, cucumber (Cucumis sativus L.) can generate both unisexual and bisexual flowers, and the sex type is mainly controlled by several 1-aminocyclopropane-1-carboxylic acid synthases (CsACSs). However, the regulatory mechanism of these synthases remains elusive. Here, we used gene expression analysis, protein–DNA interaction assays, and transgenic plants to study the function of a gynoecium-specific gene, ETHYLENE RESPONSE FACTOR31 (CsERF31), in female flower differentiation. We found that in a predetermined female flower, ethylene signaling activates CsERF31 by CsEIN3, and then CsERF31 stimulates CsACS2, which triggers a positive feedback loop to ensure female rather than bisexual flower development. A similar interplay is functionally conserved in melon (Cucumis melo L.). Knockdown of CsERF31 by RNAi causes defective bisexual flowers to replace female flowers. Ectopic expression of CsERF31 suppresses stamen development and promotes pistil development in male flowers, demonstrating that CsERF31 functions as a sex switch. Taken together, our data confirm that CsERF31 represents the molecular link between female–male determination and female–bisexual determination, and provide mechanistic insight into how ethylene promotes female flowers, rather than bisexual flowers, in cucumber sex determination.
A Mixed-Methods Investigation into Patients’ Decisions to Attend an Emergency Department for Chronic Pain
Authors: Brady Bernadette , Andary Toni , Pang Sheng Min , Dennis Sarah , Liamputtong Pranee , Boland Robert , Tcharkhedian Elise , Jennings Matthew , Pavlovic Natalie , Zind Marguerite , Middleton Paul , Chipchase Lucy 
Published: March 2021

AbstractObjectiveThis study explored factors that underpin decisions to seek emergency department (ED) care for chronic noncancer pain in patients identifying as culturally and linguistically diverse (CALD) or Australian born.Design and MethodsThis mixed-methods study was underpinned by the Behavioral Model of Health Services Use conceptual framework. Consenting consecutive patients attending the ED for a chronic pain condition were recruited to a CALD (n = 45) or Australian-born (n = 45) cohort. Statistical comparisons compared the demographic, pain, health literacy, and episode of care profiles of both cohorts. Twenty-three CALD and 16 Australian-born participants consented to an audio-recorded semi-structured interview (n = 24) or focus group (n = 5 focus groups) conducted in their preferred language. Interviews were translated and transcribed into English for analysis using applied thematic analysis, guided by the conceptual framework. Data were triangulated to investigate the patterns of ED utilization and contributing factors for both cohorts.ResultsED attendance was a product of escalating distress, influenced by the degree to which participants’ perceived needs outweighed their capacity to manage their pain. This interaction was amplified by the presence of predisposing factors, including constrained social positions, trauma exposure, and biomedical health beliefs. Importantly, experiences varied between the two cohorts with higher degrees of pain catastrophizing, lower health literacy, and greater social challenges present for the CALD cohort.ConclusionThis study highlights the role contextual factors play in amplifying pain-related distress for CALD and Australian-born patients with chronic pain. The findings support a need for health care providers to recognize features of higher vulnerability and consider streamlining access to available support services.
Asking About Attitude Change
Authors: Graham Matthew H , Coppock Alexander 
Published: March 2021

Abstract
Surveys often ask respondents how information or events changed their attitudes. Does [information X] make you more or less supportive of [policy Y]? Does [scandal X] make you more or less likely to vote for [politician Y]? We show that this type of question (the change format) exhibits poor measurement properties, in large part because subjects engage in response substitution. When asked how their attitudes changed, people often report the level of their attitudes rather than the change in them. As an alternative, we propose the counterfactual format, which asks subjects what their attitude would have been in the counterfactual world in which they did not know the treatment information. Using a series of experiments embedded in four studies, we show that the counterfactual format greatly reduces bias relative to the change format.
Onset of comorbidities and flare patterns within pre-existing morbidity clusters in people with gout: 5-year primary care cohort study
Authors: Bajpai Ram , Muller Sara , Mallen Christian , Watson Lorraine , Richette Pascal , Hider Samantha L , Roddy Edward 
Published: March 2021

Abstract

Objective
To investigate the onset of comorbidities and pattern of flares over 5 years according to baseline comorbidity clusters in people with gout.

Methods
In a prospective primary care-based cohort study, adults aged ≥18 years with gout were identified from primary care medical records in 20 general practices across the West Midlands, UK and followed up over 5 years. Four clusters of participants have been defined previously according to baseline comorbidity status. The associations of (i) incident comorbidities and (ii) gout flares with baseline cluster membership were estimated using age and sex-adjusted Poisson regression and mixed effects ordinal logistic regression, respectively.

Results
The comorbidity with the highest incidence was coronary artery disease (39.2%), followed by hypertension (36.7%), chronic kidney disease stage ≥3 (18.1%), obesity (16.0%), hyperlipidaemia (11.7%), diabetes (8.8%) and cancer (8.4%). There were statistically significant associations observed between cluster membership and incidence of coronary artery disease, hyperlipidaemia, heart failure and hypertension. In each cluster, nearly one-third of participants reported two or more gout flares at each time-point. History of oligo/polyarticular flares (odds ratio [OR]= 2.16, 95% confidence interval [CI]: 1.73, 2.70) and obesity (1.66, 95% CI: 1.21, 2.25) were associated with increasing flares whereas current use of allopurinol was associated with lower risk (0.42, 95% CI: 0.34–0.53). Cluster membership was not associated with flares.

Conclusion
Substantial numbers of people in each cluster developed new comorbidities that varies by cluster membership. People also experienced multiple flares over time, but these did not differ between clusters. Clinicians should be vigilant for the development of new comorbidities in people with gout.
Mobilizing P2P Diffusion for New Agricultural Practices: Experimental Evidence from Bangladesh
Authors: Fafchamps Marcel , Islam Asad , Malek Abdul , Pakrashi Debayan 
Published: March 2021

Abstract
This paper uses a randomized controlled experiment in which farmers trained on a new rice cultivation method teach two other farmers. The results show that the intervention increases yields and farm profits among treated farmers. Teacher-trainees are effective at spreading knowledge and inducing adoption relative to just training. Incentivizing teacher-trainees improves knowledge transmission but not adoption. Matching teacher-trainees with farmers who list them as role models does not improve knowledge transmission and may hurt adoption. Using mediation analysis, the study finds that the knowledge of the teacher-trainee is correlated with that of their students, consistent with knowledge transmission. The paper also finds that systems of rice intensification (SRI) knowledge predicts adoption of some SRI practices, and that adoption by teacher-trainees predicts adoption by their students, suggesting that students follow the example of their teacher. With cost-benefit estimates of social returns in excess of 100 percent, explicitly mobilizing peer-to-peer (P2P) transmission of knowledge seems a cost-effective way of inducing the adoption of new profitable agricultural practices.
Mixed conjugated linoleic acid sex‐dependently reverses high‐fat diet‐induced insulin resistance via the gut‐adipose axis
Authors: Li Haoyu , Zhuang Pan , Zhang Yu , Shou Qiyang , Lu Yanhua , Wang Guangfa , Qiu Jieni , Wang Jun , He Lilin , Chen Jingnan , Jiao Jingjing 
Published: March 2021

AbstractConjugated linoleic acid (CLA) may prevent the development of obesity and metabolic disorders. However, the effects of CLA on inflammation and glucose metabolism are controversial. The underlying mechanisms governing the gut microbiota and sexual dimorphisms have also not been elucidated. The present study assessed the effect of CLA on glucose and lipid metabolism in established obesity and examined the mechanism of action based on gut microbiota. Four‐week‐old C57BL/6J mice were fed a high‐fat diet (HFD) for 10 weeks to induce obesity. The diet‐induced obese (DIO) mice were fed an HFD supplemented with mixed CLA (50% cis‐9, trans‐11 isomer and 50% trans‐10, cis‐12 isomers, 0.2% wt/wt) for 15 weeks. CLA supplementation remarkably reversed body weight in both sexes. CLA favored anti‐inflammatory microbiota in male mice, mediating increased short‐chain fatty acids and decreased lipopolysaccharide (LPS) production, which alleviated global inflammation and improved insulin sensitivity via inhibition of the TLR4‐NF‐κB pathway in adipose tissue. CLA promoted the growth of hydrogen sulfide‐producing Desulfovibrio and the release of LPS in female mice, which aggravated adipose inflammation and insulin resistance. Although CLA impaired glucose metabolism in females, brown adipose tissue was significantly activated with browning of white adipose tissue in both sexes, which led to enhanced energy expenditure. Fecal transplantation from CLA‐treated mice to DIO mice mimicked the sex‐dependent phenotype. In conclusion, CLA decreased body weight and increased energy expenditure but sex‐dependently modulated insulin resistance via the gut‐adipose axis.
ACLS interventional skills: Are we as good as we should be?
Authors: Good Jason J. , Rabener Michael J. 
Published: March 2021

ABSTRACT

Objective:
This study evaluated the competence of Advanced Cardiac Life Support certified personnel at hands-on ACLS skills.

Methods:
The observational, cross-sectional study assessed participants' subjective confidence and objective skills using the ACLS mega code examination. Testing was performed with a Laerdal manikin and standardized code carts.

Results:
Participants had a 12% (6% to 22%, 95% CI) pass rate for the stable tachyarrhythmia scenario and a 57% (44% to 69%, 95% CI) pass rate for the unstable tachyarrhythmia scenario. The most significant skills missed were appropriate medications and postconversion maintenance in the stable scenario and appropriate energy selection and successful shock delivery for the unstable scenario.

Conclusions:
ACLS providers feel confident in their ACLS skills; however, actual performance demonstrated poor performance in the management of patients with stable and unstable tachyarrhythmias. The recommendation is to observe initial and recertification ACLS classes to investigate the standard to which these core skills are being taught and evaluated.
Treating breast implant-associated anaplastic large cell lymphoma
Authors: McKernan Cayla 
Published: March 2021

ABSTRACT
Each year, hundreds of thousands of women undergo aesthetic or reconstructive breast implant surgery. Clinicians and patients must be aware of the benefits and risks of this surgery. Recently, the FDA suggested a recall of certain textured breast implants because of a link between these implants and a rare form of lymphoma, now referred to as breast implant-associated anaplastic large cell lymphoma (BIA-ALCL). This malignancy has an indolent course and an excellent prognosis when detected and treated early. This article reviews BIA-ALCL, addresses public safety advisories, and emphasizes protocol that all clinicians should follow when encountering a patient with textured breast implants.
Which SSRI is safest for patients with CVD and major depressive disorder?
Authors: Dunham Sabrina , Wilkening G. Lucy , Schwier Nicholas C. 
Published: March 2021

ABSTRACT
Major depressive disorder is a common mood disorder and presents increased morbidity and mortality risks for patients with comorbid cardiovascular disease (CVD). Selective serotonin reuptake inhibitors (SSRIs) are a cornerstone of treatment for major depressive disorder, given their relative safety and affordability compared with other antidepressant classes, and SSRIs frequently are used in patients with CVD. However, clinicians should carefully weigh safety considerations before prescribing SSRIs in these patients. This article reviews the safety of SSRIs in patients with CVD and discusses SSRI selection.
Doing More With Written Feedback: Improving Learner Satisfaction and Reflection With the LEAF (Learner-Engaged Analysis of Feedback) Method
Authors: Saddawi-Konefka Daniel , Sullivan Amy , Beltran Christine , Baker Keith 
Published: March 2021

Problem
Written feedback is often overly positive, nonspecific, and difficult to interpret. Learner satisfaction with written feedback is low and obtaining written feedback that encourages self-reflection is challenging. Improving feedback quality is laborious and only modestly effective.

Approach
The authors developed the LEAF (Learner-Engaged Analysis of Feedback) method to improve learner satisfaction with, and reflection on, existing written feedback. The method pairs a learner and coach to methodically identify themes in the learner’s written feedback. Themes occurring more frequently or less frequently than typical offer areas for reflection, as they may identify learners’ relative strengths or weaknesses. The method was introduced at the Massachusetts General Hospital in 2017 during program director (PD) meetings with anesthesiology residents. In 2018, resident satisfaction was measured (1 to 5 Likert-type questions, 1 = “not at all satisfied,” 5 = “extremely satisfied”) for 4 feedback sources, 2 related to the LEAF method (PD meetings, written feedback) and 2 unrelated (verbal feedback, mentor feedback). Residents’ comments were qualitatively assessed to explore the impact on self-reflection.

Outcomes
Residents who had participated in a LEAF session (n = 54), compared with those who had not (n = 11), reported higher satisfaction with written feedback (mean 3.1 versus 2.5, d = 0.53, P = .03) and PD meeting feedback (mean 3.8 versus 2.8, d = 0.80, P = .03). There were no significant differences between groups for satisfaction with feedback unrelated to the LEAF method. Qualitative analysis of comments suggested that residents found the method useful for providing holistic self-assessment, facilitating goal setting, uncovering blind spots, and improving feedback interpretation.

Next Steps
Next steps should include studies determining if the association between increased learner satisfaction with written feedback and the LEAF method is causal, and whether this feedback process changes learners’ subsequent behaviors.
Age Effects of Bone Conduction Vibration Vestibular-evoked Myogenic Potentials (VEMPs) Using B81 and Impulse Hammer Stimuli
Authors: Patterson Jessie N. , Rodriguez Amanda I. , Gordon Katherine R. , Honaker Julie A. , Janky Kristen L. 
Published: March 2021

Objective:
Recently developed, the Radioear B81 bone oscillator allows for higher bone conduction vibration output; however, normative data are lacking regarding its use in vestibular-evoked myogenic potential (VEMP) testing. The purpose of this study was to examine the effect of age on cervical and ocular VEMP (c- and oVEMP) responses using the B81 and to compare with air conduction stimuli (ACS) and impulse hammer (IH) VEMP response characteristics.

Design:
c- and oVEMP were completed with ACS, B81, and IH stimuli in healthy participants (age range = 10 to 87 years, n = 85).

Results:
Regardless of stimulus type, c- and oVEMP amplitudes and response rates decreased with age. For cVEMP response rates, ACS performed better or equal to B81, which was superior to the IH. For cVEMP corrected amplitude, ACS had significantly higher amplitudes compared with B81 and IH. There was no difference in cVEMP corrected amplitude between B81 and IH. For oVEMP, response rates were comparable between stimuli with the largest disparity in response rates occurring in the oldest groups where IH outperformed both ACS and B81. For oVEMP amplitude, IH had significantly higher amplitudes compared with B81 and ACS. There was no difference in oVEMP amplitude between B81 and ACS.

Conclusions:
Age significantly affected c- and oVEMP amplitudes regardless of stimulus type (ACS, B81, IH). All stimuli are appropriate for eliciting c- and oVEMP in the young individuals. While ACS resulted in higher cVEMP corrected amplitudes, either ACS or B81 are appropriate for older individuals. However, for oVEMPs, higher response rates and larger amplitudes were noted for IH followed by B81 and ACS. Overall, the B81 performed well across the lifespan for c- and oVEMPs and may be a reasonable bone conduction vibration option for patients with absent ACS VEMPs, but at this time is not recommended as a replacement to ACS.
Optimizing Ocular Vestibular Evoked Myogenic Potentials With Narrow Band CE-Chirps
Authors: Mat Quentin , Duterme Jean-Pierre , Tainmont Sophie , Lelubre Christophe , Manto Mario 
Published: March 2021

Objectives:
To evaluate the effects of narrow band CE-Chirp (NB CE-Chirp) on the amplitudes and latencies in ocular vestibular evoked myogenic potentials (oVEMPs) at 500 and 1000 Hz in comparison with tone burst (TB).

Design:
Twenty-one healthy volunteers were included in the study and tested in air conduction with a “belly-tendon” montage. Recording conditions were randomized for each participant and each modality was tested twice to check the reproducibility of the procedure.

Results:
NB CE-Chirps at 500 Hz revealed larger n1-p1 amplitudes than 500 Hz TBs (p = .001), which were also larger than NB CE-Chirps and TBs at 1000 Hz (p = .022, p < .001, respectively). Besides, n1 and p1 latencies were shorter in NB CE-Chirp than in TB at 500 Hz (p < .001) and 1000 Hz (p < .001). The older the participants, the lower the amplitudes (p = .021, p = .031) and the longer the n1 (p = .030, p = .025) and p1 latencies (p < .001, p < .001) in 500 Hz NB CE-Chirps and 500 Hz TBs. Interaural asymmetry ratios were slightly higher in 500 Hz NB CE-Chirps as compared to 500 Hz TBs (p = .013).

Conclusions:
NB CE-Chirps at 500 Hz improved the amplitudes of waveforms in oVEMPs. As for TBs with clicks before, enhancing oVEMPs amplitudes is an essential step to distinguish a healthy person from a patient with either utricular or its related pathways disorder and potentially minimize the risk of cochlear damages. Additional studies including a higher number of healthy participants and patients with vestibular disorders are required to confirm this hypothesis. The large interindividual variability of interaural asymmetry ratios in NB CE-Chirp and in TB at 500 Hz could be explained by the selected montage.