bluuebunny/crossref_metadata_2025_split huggingface dataset:
The organization of cholinergic projections in the visual thalamus of the mouse
Authors: Sokhadze Guela , Whyland Kyle L. , Bickford Martha E. , Guido William 
Published: August 2021

AbstractCholinergic projections from the brainstem serve as important modulators of activity in visual thalamic nuclei such as the dorsal lateral geniculate nucleus (dLGN). While these projections have been studied in several mammals, a comprehensive examination of their organization in the mouse is lacking. We used the retrograde transport of viruses or cholera toxin subunit B (CTB) injected in the dLGN, immunocytochemical labeling with antibodies against choline acetyltransferase (ChAT), brain nitric oxide synthase (BNOS), and vesicular acetylcholine transporter (VAChT), ChAT‐Cre mice crossed with a reporter line (Ai9), as well as brainstem virus injections in ChAT‐Cre mice to examine the pattern of thalamic innervation from cholinergic neurons in the pedunculopontine tegmental nucleus (PPTg), laterodorsal tegmental nucleus (LDTg), and the parabigeminal nucleus (PBG). Retrograde tracing demonstrated that the dLGN receives input from the PPTg, LDTg, and PBG. Viral tracing in ChAT‐Cre mice and retrograde tracing combined with immunocytochemistry revealed that many of these inputs originate from cholinergic neurons in the PBG and PPTg. Most notable was an extensive cholinergic projection from the PBG which innervated most of the contralateral dLGN, with an especially dense concentration in the dorsolateral shell, as well as a small region in the dorsomedial pole of the ipsilateral dLGN. The PPTg was found to provide a sparse somewhat diffuse innervation of the ipsilateral dLGN. Neurons in the PPTg co‐expressed ChAT, BNOS, and VAChT, whereas PBG neurons expressed ChAT, but not BNOS or VAChT. These results highlight the presence of distinct cholinergic populations that innervate the mouse dLGN.
Human leukocyte antigens are associated with salivary level of active MMP‐8
Authors: Mauramo Matti , Mauramo Elina , Sorsa Timo , Tervahartiala Taina , Räisänen Ismo T. , Waltimo Tuomas 
Published: August 2021

AbstractObjectivesThis case control study examined the associations of HLA antigens and periodontitis with the salivary level of active MMP‐8 (aMMP‐8).Materials and MethodsA total of 202 subjects, registered as Swiss bone marrow donors, participated in the study. HLA‐A, ‐B, and ‐C types were determined by serology or PCR. Saliva samples were collected from subjects, followed by a periodontal examination. The salivary level of aMMP‐8 was determined with immunofluorometric assay.ResultsThe mean salivary level of aMMP‐8 was directly comparable to the grade of periodontitis and increased from healthy to mild/moderate to severe (125.0 ± 132.1, 200.6 ± 170.2, 290.1 ± 202.3 ng/ml; p < 0.001 between each group, respectively). The only association between the HLA types and the salivary level of aMMP‐8 was observed in subjects with HLA‐A11. Subjects with healthy periodontium and HLA‐A11 had a lower level of aMMP‐8 (49.2 ± 32.5 ng/ml) compared with subjects without HLA‐A11 (123.6 ± 119.2; p = 0.048). Among subjects with periodontitis, a higher level of aMMP‐8 (394.2 ± 255.6 ng/ml) was observed in subjects with HLA‐A11 compared with subjects without HLA‐A11 (201.1 ± 146.1 ng/ml; p < 0.002). This finding was statistically significant also after adjusting for sex, age, smoking, tooth brushing and the number of medications (p < 0.05).ConclusionsHLA‐A11 is associated with the salivary level of aMMP‐8 which contributes to the subject's immune and inflammatory response in periodontium.
Performance measurement tools for sustainable business: A systematic literature review on the sustainability balanced scorecard use
Authors: Mio Chiara , Costantini Antonio , Panfilo Silvia 
Published: August 2021

AbstractThis article focuses on the sustainability balanced scorecard (SBSC) as a performance measurement and management control tool that can play an essential role in driving companies towards sustainability goals. According to previous studies, research on the SBSC can be structured into the stages of design, implementation, use and evolution. This study aims to systematise knowledge on the use stage. Specifically, it addresses the determinants affecting SBSC use, the approaches that companies employ in SBSC application and the outcomes it generates in terms of the effects on sustainability control and management. The research was conducted through a systematic literature review considering 65 articles published in ABS‐ranked journals in the period 2000–2020. Findings add to the body of literature on the SBSC in management and accounting fields, providing an overview of current research, mapping research streams, indicating potential future research avenues and highlighting some managerial implications.
Effects of animal grazing on vegetation biomass and soil moisture on a typical steppe in Inner Mongolia, China
Authors: Lin Xi , Zhao Hongbin , Zhang Shengwei , Li Xiaoyuan , Gao Wenlong , Ren Zhaoyi , Luo Meng 
Published: August 2021

AbstractGrasslands play a very important role in the water and carbon cycle of arid and semi‐arid areas, where they are the main type of steppe globally. With this said, research into grassland ecological processes mostly consists of single‐factor controlled experiments such as precipitation, temperature or grazing, and few studies have investigated the effects of synergistic interactions between multiple factors on grassland hydrological, soil and vegetation processes. In this study, we set up a prohibited grazing area in a typical area of the Xilingole Steppe in Inner Mongolia, China. Vegetation (species richness (SR), above‐ground biomass (AGB), below‐ground biomass (BGB), etc.), precipitation and the soil moisture of 5 cm, 10 cm, 15 cm and 30 cm depths were observed continuously from 2015 to 2018. The results indicate that the species number in areas where grazing is prohibited is higher than where it is grazed, and the number and species of dominant species changes with grazing prohibition time. The AGB and BGB of prohibited areas is higher than grazed areas, and the variance rate of AGB increased rapidly (20% and 45%) at the first 2 years and then stabilized (52% and 55%), but BGB's variance rate increased slowly from 12% to 20%. The soil moisture content in the study area is higher in the surface layer than in the deeper layers. In the grazing prohibition zone, the above ground biomass (AGB) and belowground biomass (BGB) were both significantly correlated with volumetric water content (VWC) at the 0.01 level. In grazing areas, there was no significant correlation between AGB and soil moisture, and the coefficient of determination between BGB and VWC was 0.6127 (p < 0.01). SR did not have a significant relationship with soil moisture but indirectly response to it through BGB, especially in prohibited site. These results are important for understanding water cycle processes, grazing management and address food security issues across steppe in arid and semi‐arid regions.
Plant traits mediate the effects of climate on phytophagous beetle diversity on Mt. Kilimanjaro
Authors: Njovu Henry K. , Steffan‐Dewenter Ingolf , Gebert Friederike , Schellenberger Costa David , Kleyer Michael , Wagner Thomas , Peters Marcell K. 
Published: August 2021

AbstractPatterns of insect diversity along elevational gradients are well described in ecology. However, it remains little tested how variation in the quantity, quality, and diversity of food resources influence these patterns. Here we analyzed the direct and indirect effects of climate, food quantity (estimated by net primary productivity), quality (variation in the specific leaf area index, leaf nitrogen to phosphorus and leaf carbon to nitrogen ratio), and food diversity (diversity of leaf traits) on the species richness of phytophagous beetles along the broad elevation and land use gradients of Mt. Kilimanjaro, Tanzania. We sampled beetles at 65 study sites located in both natural and anthropogenic habitats, ranging from 866 to 4,550 m asl. We used path analysis to unravel the direct and indirect effects of predictor variables on species richness. In total, 3,154 phytophagous beetles representing 19 families and 304 morphospecies were collected. We found that the species richness of phytophagous beetles was bimodally distributed along the elevation gradient with peaks at the lowest (˜866 m asl) and upper mid‐elevations (˜3,200 m asl) and sharply declined at higher elevations. Path analysis revealed temperature‐ and climate‐driven changes in primary productivity and leaf trait diversity to be the best predictors of changes in the species richness of phytophagous beetles. Species richness increased with increases in mean annual temperature, primary productivity, and with increases in the diversity of leaf traits of local ecosystems. Our study demonstrates that, apart from temperature, the quantity and diversity of food resources play a major role in shaping diversity gradients of phytophagous insects. Drivers of global change, leading to a change of leaf traits and causing reductions in plant diversity and productivity, may consequently reduce the diversity of herbivore assemblages.
Water Addition During Oil Extraction Affects on Virgin Olive Oil Ethanol Content, Quality and Composition
Authors: Beltrán Gabriel , Bejaoui Mohamed Aymen , Sánchez‐Ortiz Araceli , Jiménez Antonio 
Published: August 2021

AbstractEthanol is the alcoholic precursor of fatty acid ethyl esters (FAEEs) in virgin olive oil (VOO). Because of its miscibility, water addition during oil extraction may affect oil ethanol content and then, the FAEEs synthesis during oil storage. In this work, the effect of water addition on VOO ethanol content and composition is studied. Water addition at two extraction systems (two and three phases) is compared and for vertical centrifuge, water addition at different temperatures is assayed. Ethanol content, quality parameters, and healthy components are determined in the oils. Results indicate three phase system gives oils with a 25% lower ethanol content than two phases. Ethanol reduction because of water addition is more important for three phases system (≈14%). For vertical centrifugation, ethanol is lowered as water dose and temperature increase. In general, water addition for any of the extraction steps analyzed reduces the oil ethanol concentration but other aspects such as fruity intensity and phenol content are also lowered.Practical applications: Virgin olive oil final ethanol content, and then its FAEEs concentration, does not only depend on the health and conservation status of olives, but also on the extraction system used and the amount of water added to the extraction process. The knowledge of the impact on ethanol content of water addition during oil extraction can be useful for olive oil legislators in order to keep the approved limits of FAEEs or to modify them. For oil producers, results can help to reduce the oil ethanol content and then FAEEs synthesis during virgin olive oil storage.
Synthesis, Reactivity and Activation Modes of Fluoroalkyl Thiosulfonates and Selenosulfonates
Authors: Ghiazza Clément , Billard Thierry 
Published: August 2021

AbstractReagent design is at the cornerstone of modern organofluorine chemistry. The past decades have seen the emergence of diverse families of reagents with the aim of introducing fluoroalkylthio or seleno groups in an efficient and selective way. Among them, highly versatile fluoroalkyl thiosulfonates and selenosulfonates are in the foreground of the recent developments. Their applicability is discussed in detail in this Minireview, with a specific focus on the reactivity and activation modes.
Cortical network organization reflects clinical response to subthalamic nucleus deep brain stimulation in Parkinson's disease
Authors: Bočková Martina , Výtvarová Eva , Lamoš Martin , Klimeš Petr , Jurák Pavel , Halámek Josef , Goldemundová Sabina , Baláž Marek , Rektor Ivan 
Published: August 2021

AbstractThe degree of response to subthalamic nucleus deep brain stimulation (STN‐DBS) is individual and hardly predictable. We hypothesized that DBS‐related changes in cortical network organization are related to the clinical effect. Network analysis based on graph theory was used to evaluate the high‐density electroencephalography (HDEEG) recorded during a visual three‐stimuli paradigm in 32 Parkinson's disease (PD) patients treated by STN‐DBS in stimulation “off” and “on” states. Preprocessed scalp data were reconstructed into the source space and correlated to the behavioral parameters. In the majority of patients (n = 26), STN‐DBS did not lead to changes in global network organization in large‐scale brain networks. In a subgroup of suboptimal responders (n = 6), identified according to reaction times (RT) and clinical parameters (lower Unified Parkinson's Disease Rating Scale [UPDRS] score improvement after DBS and worse performance in memory tests), decreased global connectivity in the 1–8 Hz frequency range and regional node strength in frontal areas were detected. The important role of the supplementary motor area for the optimal DBS response was demonstrated by the increased node strength and eigenvector centrality in good responders. This response was missing in the suboptimal responders. Cortical topologic architecture is modified by the response to STN‐DBS leading to a dysfunction of the large‐scale networks in suboptimal responders.
Extracellular vesicles derived from mesenchymal stem/stromal cells: The regenerative impact in liver diseases
Authors: Psaraki Adriana , Ntari Lydia , Karakostas Christos , Korrou‐Karava Despoina , Roubelakis Maria G. 
Published: August 2021

Abstract
Liver dysfunctions are classified into acute and chronic diseases, which comprise a heterogeneous group of pathological features and a high mortality rate. Liver transplantation remains the gold‐standard therapy for most liver diseases, with concomitant limitations related to donor organ shortage and lifelong immunosuppressive therapy. A concept in liver therapy intends to overcome these limitations based on the secreted extracellular vesicles (EVs; microvesicles and exosomes) by mesenchymal stem/stromal cells (MSCs). A significant number of studies have shown that factors released by MSCs could induce liver repair and ameliorate systemic inflammation through paracrine effects. It is well known that this paracrine action is based not only on the secretion of cytokines and growth factors but also on EVs, which regulate pathways associated with inflammation, hepatic fibrosis, integrin‐linked protein kinase signaling, and apoptosis. Herein, we extensively discuss the differential effects of MSC‐EVs on different liver diseases and on cellular and animal models and address the complex molecular mechanisms involved in the therapeutic potential of EVs. In addition, we cover the crucial information regarding the type of molecules contained in MSC‐EVs that can be effective in the context of liver diseases. In conclusion, outcomes on MSC‐EV‐mediated therapy are expected to lead to an innovative, cell‐free, noninvasive, less immunogenic, and nontoxic alternative strategy for liver treatment and to provide important mechanistic information on the reparative function of liver cells.
Morphometric Analysis of Mast Cells in Tumor Predicts Recurrence of Hepatocellular Carcinoma After Liver Transplantation
Authors: Rohr‐Udilova Nataliya , Tsuchiya Kaoru , Timelthaler Gerald , Salzmann Martina , Meischl Tobias , Wöran Katharina , Stift Judith , Herac Merima , Schulte‐Hermann Rolf , Peck‐Radosavljevic Markus , Sieghart Wolfgang , Eferl Robert , Jensen‐Jarolim Erika , Trauner Michael , Pinter Matthias 
Published: August 2021

Tumor‐infiltrating immune cells are relevant prognostic and immunotherapeutic targets in hepatocellular carcinoma (HCC). Mast cells play a key role in allergic response but may also be involved in anticancer immunity. Digital morphometric analysis of patient tissue sections has become increasingly available for clinical routine and provides unbiased quantitative data. Here, we apply morphometric analysis of mast cells to retrospectively evaluate their relevance for HCC recurrence in patients after orthotopic liver transplantation (OLT). A total of 173 patients underwent OLT for HCC at the Medical University of Vienna (21 women, 152 men; 55.2 ± 7.9 years; 74 beyond Milan criteria, 49 beyond up‐to‐7 criteria for liver transplantation). Tissue arrays from tumors and corresponding surrounding tissues were immunohistochemically stained for mast cell tryptase. Mast cells were quantified by digital tissue morphometric analysis and correlated with HCC recurrence. Mast cells were detected in 93% of HCC tumors and in all available surrounding liver tissues. Tumor tissues revealed lower mast cell density than corresponding surrounding tissues (P < 0.0001). Patients lacking intratumoral mast cells (iMCs) displayed larger tumors and higher tumor recurrence rates both in the whole cohort (hazard ratio [HR], 2.74; 95% confidence interval [CI], 1.09‐6.93; P = 0.029) and in patients beyond transplant criteria (Milan HR, 2.81; 95% CI, 1.04‐7.62; P = 0.01; up‐to‐7 HR, 3.58; 95% CI, 1.17‐10.92; P = 0.02). Notably, high iMC identified additional patients at low risk classified outside the Milan and up‐to‐7 criteria, whereas low iMC identified additional patients at high risk classified within the alpha‐fetoprotein French and Metroticket criteria. iMCs independently predicted tumor recurrence in a multivariate Cox regression analysis (Milan HR, 2.38; 95% CI, 1.16‐4.91; P = 0.019; up‐to‐7 HR, 2.21; 95% CI, 1.05‐4.62; P = 0.035). Conclusion: Hepatic mast cells might be implicated in antitumor immunity in HCC. Morphometric analysis of iMCs refines prognosis of HCC recurrence after liver transplantation.
Hippocampal subfield volumes and memory discrimination in the developing brain
Authors: Bouyeure Antoine , Patil Sandesh , Mauconduit Franck , Poiret Clément , Isai Damien , Noulhiane Marion 
Published: August 2021

AbstractThe ability to keep distinct memories of similar events is underpinned by a type of neural computation called pattern separation (PS). Children typically report coarse‐grained memories narratives lacking specificity and detail. This lack of memory specificity is illustrative of an immature or impaired PS. Despite its importance for the ontogeny of memory, data regarding the maturation of PS during childhood is still scarce. PS is known to rely on the hippocampus, particularly on hippocampal subfields DG and CA3. In this study, we used a memory discrimination task, a behavioral proxy for PS, and manually segmented hippocampal subfields volumes in the hippocampal body in a cohort of 26 children aged from 5 to 12 years. We examined the association between subfields volumes and memory discrimination performance. The main results were: (1) we showed age‐related differences of memory discrimination suggesting a continuous increase of memory performance during early to late childhood. (2) We evidenced distinct associations between age and the volumes of hippocampal subfield, suggesting distinct developmental trajectories. (3) We showed a relationship between memory discrimination performance and the volumes of CA3 and subiculum. Our results further confirm the role of CA3 in memory discrimination, and suggest to scrutinize more closely the role of the subiculum. Overall, we showed that hippocampal subfields contribute distinctively to PS during development.
Increasing Olefin Metathesis Activity of Silica‐Supported Molybdenum Imido Adamantylidene Complexes through E Ligand σ‐Donation
Authors: Nater Darryl F. , Paul Bhaskar , Lätsch Lukas , Schrock Richard R. , Copéret Christophe 
Published: August 2021

AbstractMolybdenum imido adamantylidene complexes with different substituents on the imido ligand (dipp=2,6‐diisopropylphenyl, ArF5=C6F5, and tBu) having distinct electron donating abilities were investigated for the metathesis of internal and terminal olefins, for both molecular and silica‐supported species using standardized protocols. Here we show that surface immobilization of these compounds results in dramatically increased activity compared to their molecular counterparts. Additionally, we show that electron withdrawing imido groups increase the activity of the compound towards terminal olefins while they simultaneously decrease the ability to metathesize internal olefins. Furthermore, these systems also show high stability when used as initiators in olefin metathesis, although the species that display higher initial activity deactivate faster than those that show more a more moderate reaction rate at first. Our catalytic studies, augmented by DFT calculations, show that all investigated compounds have a remarkably small energy difference between the trigonal bipyramidal (TBP) and square planar (SP) configurations of the metallacyclobutane intermediates, which has previously been linked to high activity.
Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
Authors: Yuan Qing , Wang Song‐Quan , Zhang Guang‐Tao , He Jie , Liu Zhi‐Dan , Wang Ming‐Rong , Cai Hong‐Qing , Wan Jing‐Hai 
Published: August 2021

AbstractIntroductionThe prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression in the biological process, prognosis, and immune significance in glioma.MethodsWe analyzed the biological functions of SERPINA3 through data from the Chinese Glioma Genome Atlas databases. Differentially expressed genes and enrichment analysis were performed and correlations between SERPINA3 expression and immune cell infiltration were analyzed. Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. The correlations between the expression and clinical‐pathological parameters as well as other biomarkers were examined.ResultsUnivariate and multivariate regression both indicated that the level of SERPINA3 transcript represented an independent prognostic factor. High levels of SERPINA3 correlated with poor survival in patients with glioma. Expression of SERPINA3 mRNA was observed positively correlated with MCM6, IGFBP2, and FKBP10. Enrichment analysis showed SERPINA3 mainly enriched in immune‐related terms and signaling pathways including MAPK, TNF, P53, PI3K‐Akt, nuclear factor‐κB. Immune infiltration analysis further declare the SERPINA3 expression negatively correlated with levels of Macrophages M1, native CD4+ T cell, monocytes, and Mast cell activated. And overexpression of SERPINA3 correlated with low CD4+ T cell infiltration in glioma tissues.ConclusionsSERPINA3 may play a key role in the biological process of glioma cells especially in immune suppression activities. SERPINA3 may serve as an independent survival prediction factor in glioma patients.