bluuebunny/crossref_metadata_2025_split huggingface dataset:
An ancestral Wnt-Brachyury feedback loop and recruitment of mesoderm-determining target genes revealed by comparative Brachyury target screens
Authors: Schwaiger Michaela , Andrikou Carmen , Dnyansagar Rohit , Murguia Patricio Ferrer , Paganos Periklis , Voronov Danila , Zimmermann Bob , Lebedeva Tatiana , Schmidt Heiko , Genikhovich Grigory , Benvenuto Giovanna , Arnone Maria , Technau Ulrich 
Published: August 2021

Abstract
Transcription factors are crucial drivers of cellular differentiation during animal development and often share ancient evolutionary origins. The T-box transcription factor Brachyury plays a pivotal role as an early mesoderm determinant and neural repressor in vertebrates; yet, the ancestral function and key evolutionary transitions of the role of this transcription factor remain obscure. Here, we present a genome-wide target gene screen using ChIP-seq in the sea anemone Nematostella vectensis, an early branching non-bilaterian, and the sea urchin Strongylocentrotus purpuratus, a representative of the sister lineage of chordates. Our analysis reveals an ancestral gene regulatory feedback loop connecting Brachyury, FoxA and canonical Wnt signaling involved in axial patterning that predates the cnidarian-bilaterian split about 700 million years ago. Surprisingly, we also found that part of the gene regulatory network controlling the fate of neuromesodermal progenitors in vertebrates was already present in the common ancestor of cnidarians and bilaterians. However, while several neuronal Brachyury target genes are ancestrally shared, hardly any of the key mesodermal downstream targets in vertebrates are found in the sea anemone or the sea urchin. Our study suggests that a limited number of target genes involved in mesoderm formation were newly acquired in the vertebrate lineage, leading to a dramatic shift in the function of this ancestral developmental regulator.
AFP and CA-125 as an accurate risk factor to predict eye metastasis in hypertension patients with liver Carcinoma： A STROBE-compliant article
Authors: Tang Jing , Zhang Li-Juan , Huang Yan-Cheng , Huang Rong , Shu Hui-Ye , Wu Jie-Li , Li Qiu-Yu , Su Ting , Pan Yi-Cong , Liang Rong-Bin , Shao Yi 
Published: August 2021

Abstract
Purpose
Primary liver carcinoma is a common malignant tumor. In this study, we analyzed the differences between hypertension patients with ocular metastasis of liver cancer and those with metastases to other sites, the correlation between history of HBV and liver cancer metastasis, and independent risk factors for ocular metastasis.
METHODS
We used treatment records from 488 patients with metastases of primary liver cancer from August 2001 to May 2015, divided into two groups based on metastatic sites: OM (ocular metastasis) and NOM (non-ocular, other sites of metastasis) groups. The Student’s t-test and Chi-square test were used to assess the significance of differences between the groups and define the relationship between history of HBV and ocular metastasis of liver cancer. Binary logistic regression analysis was used to identify indicators of ocular metastasis of liver cancer and receiver operating curve (ROC) analyses to estimate their diagnostic value.
RESULTS
No significant differences in sex, age, tumor stage, pathological type, or treatment were identified between the OM and NOM groups, while the prevalence of HBV was higher in the former than that in latter, confirming the association between history of HBV and ocular metastasis. Binary logistic regression demonstrated that AFP and CA-125 were independent indicators of liver metastasis (both P < 0.001). ROC curve analyses generated cut-off values for AFP and CA-125 of 957.2 ng/ml and 114.25 U/ml, respectively, with corresponding AUC values of 0.739 and 0.810. The specificity of the combination of AFP and CA-125 was higher than either factor separately.
Compatibilization and Toughening of Biodegradable Poly (Lactic Acid) / Cellulose Acetate Films by Polyamide Amine Dendrimers
Authors: Huang Yansong , Jin Yu juan , Wang Bo , Tian Huafeng , Weng Yunxuan , Men Shuang 
Published: August 2021

Abstract
Due to the poor compatibility caused by the large difference in hydrophilicity and interface between polylactic acid (PLA) and cellulose acetate (CA), the blending of the two materials is difficult and the application is limited. To solve this problem, a type of polyamide amine (PAMAM) dendrimer was introduced to modify PLA / CA blends in this work. The results showed that PAMAM could improve the compatibility of PLA / CA blends, promote the distribution of CA and crystallization of PLA. At the same time, adding PAMAM could enhance the mechanical properties of the blend material, and the toughness and tear strength were increased by 551% and 141%, respectively. In addition, the incorporation of PAMAM increased hydrophobicity and oxygen permeability of PLA/ CA blends, and the oxygen permeability could be increased by up to 3 orders of magnitude. Degradation test results showed that the blend exhibited good biodegradability. The overall performance of the PLA/ CA blend film was optimal when the content of PAMAM was 3 phr. The biodegradable blend films with excellent performance provided wide application prospect in the food green packaging fields.
Towards Big Industrial Data Mining Through Explainable Automated Machine Learning
Authors: Garouani Moncef , Ahmad Adeel , Bouneffa Mourad , Hamlich Mohamed , Bourguin Gregory , Lewandowski Arnaud 
Published: August 2021

Abstract
Industrial systems resources are capable of producing large amount of data. These data are often in heterogeneous formats and distributed, yet they provide means to mine the information which can allow the deployment of intelligent management tools for production activities. For this purpose, it is necessary to be able to implement knowledge extraction and prediction processes using Artificial Intelligence(AI) models but the selection and configuration of intended AI models tend to be increasingly complex for a non-expert user. In this paper, we present an approach and a software platform that may allow industrial actors, who are usually not familiar with AI, to select and configure algorithms optimally adapted to their needs. Hence, the approach is essentially based on automated machine learning. The resulting platform effectively enables a better choice among the combination of AI algorithms and hyper-parameter configurations. It also makes it possible to provide features of explainability of the resulting algorithms and models, thus increasing the acceptability of these models in practicing community of the users. The proposed approach has been applied in the field of predictive maintenance. Current tests are based on the analysis of more than 360 databases from the subjected field.
Nowcasting transmission and suppression of the Delta variant of SARS-CoV-2 in Australia
Authors: Chang Sheryl , Cliff Oliver , Zachreson Cameron , Prokopenko Mikhail 
Published: August 2021

Abstract
As of July 2021, there is a continuing outbreak of the B.1.617.2 (Delta) variant of SARS-CoV-2 in Sydney, Australia. The outbreak is of major concern as the Delta variant is estimated to have twice the reproductive number of previous variants that circulated in Australia in 2020, which is worsened by low levels of acquired immunity in the population. Using a re-calibrated agent-based model, we explored a feasible range of non-pharmaceutical interventions, in terms of both mitigation (case isolation, home quarantine) and suppression (school closures, social distancing). Our nowcasting modelling indicates that the level of social distancing currently attained in Sydney is inadequate for the outbreak control. A counter-factual analysis suggests that if 80% of agents comply with social distancing, then at least a month is needed for the new daily cases to reduce from their peak to below ten. A small reduction in social distancing compliance to 70% lengthens this period to 45 days.
Immunogenicity and efficacy of heterologous ChadOx1/BNT162b2 vaccination
Authors: pozzetto bruno , legros vincent , Djebali Sophia , barateau véronique , guibert nicolas , Villard Marine , Peyrot Loïc , allatif omran , fassier jean-baptise , Massardier-Pilonchéry Amélie , Brengel-Pesce Karen , Yaugel-Novoa Melyssa , Denolly Solène , boson bertrand , Bourlet Thomas , Valette Martine , Andrieu Thibault , Lina Bruno , group Lyon COVID study , Cosset François-Loïc , Paul Stéphane , Defrance Thierry , Marvel Jacqueline , Walzer Thierry , Trouillet-Assant Sophie 
Published: August 2021

Abstract
Following severe adverse reactions in patients vaccinated with the AstraZeneca ChadOx1 (Chad) vaccine, European health authorities have recommended that patients under the age of 55 who received one dose of Chad vaccine receive a second dose of Pfizer BNT162b2 (BNT) vaccine as a booster. However, the effectiveness and the immunogenicity of this vaccination regimen have not been formally tested. Here, we show that the heterologous Chad/BNT combination confers better protection against SARS-CoV-2 infection than the homologous BNT/BNT combination in a population of health care workers. To understand the underlying mechanism, we monitored in a longitudinal way the anti-spike immunity conferred by each vaccinal combination. Both combinations induced strong anti-spike antibody responses after boost in all vaccinated individuals. However, sera from heterologous vaccinated individuals displayed a stronger neutralizing activity, regardless of the SARS-CoV-2 variant analyzed, and this was associated with more switched memory RBD-specific B cells with an activated phenotype and less IgA. The Chad vaccine induced a stronger T cell response than the BNT vaccine after the priming dose, and the reciprocal was true for the IgG response, which could explain the complementarity of both vaccines when used in an heterologous setting. This strongly protective vaccination regimen could be therefore particularly suitable for immunocompromised individuals.
Public Attention Reduced Forest Fires in the Brazilian Amazon
Authors: Araujo Rafael , Costa Francisco , Garg Teevrat 
Published: August 2021

Abstract
International frameworks and agreements to reduce anthropogenic environmental disasters rely on international pressure driving local action. Although environmental catastrophes can occasionally capture international attention, it is unclear if focused media and increased public outcry can reduce environmental damage. We study the unusual and concentrated increase in international scrutiny on forest fires in the Brazilian Amazon in August 2019. Comparing active fires in the Brazilian Amazon versus those in the Peruvian and Bolivian Amazon before and after a surge in public attention on the Brazilian Amazon, we find that increased public attention reduced fires by 22% (93,607 avoided pixel-days of active fire) avoiding 24.81 million MtCO2 in emissions. Our results highlight the power of international pressure to compel governments to act on pressing environmental issues, even in political contexts hostile to environmental priorities.
Research and Design the Thrust Fluctuation of Slotted-Tubular Permanent Magnet Linear Motor: an application on low frequency plastic forming equipment
Authors: Gao Jingzhou , Li Jingxiang , Zhao Shengdun , Du Wei , Jiang Fei , Zhenhao Zheng , Wu Xinchu 
Published: August 2021

Abstract
In this paper, a novel type of low frequency vibration plastic forming equipment is proposed, in which the slotted tubular permanent magnet synchronous liner motor (slotted – TPMLM) is the core component and plays the roles of power source and vibration source. So, this paper focuses on the research about the design of the slotted - TPMLM. The suppression and optimization of motor thrust fluctuation has always been a hot research issue. But that is not the purpose of this article. Here, the effect of the length of stator core on end force and the influence of the number of poles and slots on the cogging force are discussed in detail. The purpose is to design the thrust fluctuation of slotted – TPMLM reasonably so that the thrust fluctuation can be used in the low frequency plastic forming. The slotted – TPMLM is designed, manufactured, and tested. The experimental results show that the motor can output thrust with periodic fluctuation (low frequency, about 4.8 Hz), and the thrust fluctuation accounts for about 16.5% of the average thrust, which meets the metal low frequency vibration plastic forming requirements. Consequently, the low frequency vibration plastic forming equipment can be realized, which contributes to the field of the plastic forming.
The protective effects of melatonin in high glucose environment by alleviating autophagy and apoptosis on primary cortical neurons
Authors: Lijiao Xiong , Liu Song , Liu Chaoming , Guo Tianting , Huang Zhihua , Li Liangdong 
Published: August 2021

Abstract
Cognitive dysfunction has been regarded as a complication of diabetes. Melatonin shows a neuroprotective effect on various neurological diseases. However, it’s protective effect on cortical neurons in high glucose environment has not been reported. Our present study aims to observe the protective effect of melatonin on rat cortical neurons and its relationship with autophagy in high glucose environment. The rat primary cortical neurons damaged model was induced by high glucose. The CCK-8, flow cytometry, Western Blot and immunofluorescence methods were used to examine the cell viability, apoptosis rate and proteins expression. Our results showed that there were no differences in cell viability, apoptosis rate, and protein expression among the control MLT and mannitol group. The cell viability of the glucose group was significantly lower than that of the control group, and the apoptosis rate of the glucose group was significantly higher than that of the control group. Compared with the glucose group, the glucose + melatonin group showed a significant increase in cell viability and a notable decrease in apoptosis rate. Melatonin concentration of 0.1-1 mmol/L can significantly reduce the injury of cortical neurons by high glucose. Compared with the control group, the glucose group showed a significant reduction of Bcl-2 protein expression, while remarkable elevations of Bax, caspase-3, Beclin-1 and LC3B levels. The neurons pre-administered with melatonin obtained significantly reversed these changes induced by high glucose. The phosphorylation levels of Akt and mTOR in the glucose group were significantly lower than those in the control group, were significantly increased in the glucose + MLT group compared with the glucose group. These data indicated that melatonin has a neuroprotective effect on cortical neurons under high glucose environment, which may work by activating Akt/mTOR pathway and following the down-regulation of autophagy.
The effects of aerobic, strength, or mixed exercise programs on schizophrenia symptomatology: a randomized clinical trial with a 10-month follow-up
Authors: García-Garcés Laura , Sánchez-López María Inmaculada , Lacamara-Cano Sergio , Cebolla-Meliá Yago , Marqués-Azcona David , Biviá-Roig Gemma , Lisón Juan Francisco , Peyró-Gregori Loreto 
Published: August 2021

Abstract
The purpose of this study was to compare the effects of three different physical exercise programs on the symptomatology, body composition, and physical fitness of individuals with schizophrenia. A total of 432 patients were assessed for eligibility and 86 were randomized into the aerobic (n = 28), strength (n = 29) or mixed (n = 29) groups. Positive, negative, and general symptoms of psychosis, body mass index (BMI), and physical fitness (6-minute walk test [6MWT] and hand-grip strength [HGS]) were assessed at baseline, post-intervention (16 weeks), and at 10-months. Our results at 16 weeks showed significant improvements in all three groups in the negative, general, and total symptoms with moderate to large effect sizes (P < 0.01, ηp2 > 0.11), no change in the BMI or 6MWT, and a significant improvement in the HGS test in the strength and mixed groups (P ≤ 0.05, ηp2 > 0.08). Nonetheless, all the improvements had disappeared at 10 months. We concluded that 3 weekly sessions of a moderate to vigorous progressive exercise program for 16 weeks improved the symptomatology of schizophrenic patients, regardless the type of exercise they performed. However, the effects had declined to baseline levels by the 10-month follow-up, suggesting that exercise interventions should be maintained over time.
Research on Road Performance of Asphalt Mixture With Iron Waste Ore
Authors: Fei Aiping , ZHANG TIEZHI , Ruan Zhongwei 
Published: August 2021

Abstract
In order to study the road performance of asphalt mixture with different kinds of iron waste ore , stripping waste rock rich in iron trioxide and stripping waste rock rich in iron oxide are used as aggregate to make the mixture.Through the test comparison, it is confirmed that the dynamic stability performance of asphalt concrete mixture mixed with iron trioxide mining and stripping waste rock is the best, namely its high temperature stability is best; followed by the one mixed with waste rock rich in iron trioxide. The residue stability of asphalt concrete mixed with mining and stripping waste rock increases obviously. It can found from the experiments that the residual stability can be up to 94.96% which kinds of asphalt mixture with waste rock rich in iron trioxide. Asphalt mixture mixed with stripping waste rock has a better anti-sliding performance than the common one, among which the one mixed with aggregates rich in iron trioxide is the best. Through tectonic depth, it is reflected that it also has a better performance on drainage noise reduction. In conclusion, using mining and stripping waste rock instead of mining gravel has a good economic efficiency,but also solved the problem of mining and stripping waste rock accumulation.
A Systematic Review Protocol to Establish Plasmodium Falciparum Genetic Diversity, Multiplicity of Infection, Genotyping Approaches, and Methods of Reporting It in Africa.
Authors: Mwesigwa Alex , Nankabirwa Joaniter Immaculate , Nsobya Samuel Lubwama , Karamagi Charles , Castelnuovo Barbara , Byakika-Kibwika Pauline 
Published: August 2021

Abstract
BackgroundP. falciparum is the most important Plasmodium species that causes high malaria morbidity and mortality. The distribution of diverse and multiple P. falciparum infections is a major setback to the control and eventual elimination of malaria globally. Little efforts have been made to systematically establish P. falciparum genetic diversity and multiplicity of infection (MOI) in African settings. Additionally, the choice of an effective P. falciparum genotyping approach for a specific endemic setting remains a challenge. The review aims to establish P. falciparum genetic diversity MOI, genotyping approaches, and methods of reporting it in Africa. This will aid the evaluation of the impact of malaria control interventions and development of new control strategies.MethodsThe review will consider Randomized Clinical Trials (RCTs), Quasi-experiments, Cross-sectional studies, Cohort and Case-control studies about P. falciparum genetic diversity and MOI. A literature search will be conducted in PubMed, Google scholar and MEDLINE databases for articles published from January 2010 to December 2020. Articles will be screened using Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Risk of bias in RCTs will be assessed using Cochrane risk of bias assessment tool while New Castle Ottawa tools will be used to assess the risk of bias in observational studies. Publication bias will be assessed using a funnel plot as well as Begg and Mazumdar's rank correlation test or Egger's test. The Higgins I2 statistic and Galbraith plots will be used to assess heterogeneity. A meta-regression analysis will be performed to explain the low heterogeneity. DiscussionThe findings from the study will enhance our understanding of the distribution and dynamics of P. falciparum genetic diversity and MOI. This will provide insights to the changing landscape of malaria transmission and evaluation of malaria control interventions. Findings will also serve as baseline data for future studies on parasite population structure and antimalarial drug resistance surveillance across African countries.
Solid-State Optical Properties of Self-Assembling Amyloid-Like Peptides with Different Charged States at the Terminal Ends
Authors: Schiattarella Chiara , Diaferia Carlo , Gallo Enrico , Ventura Bartolomeo Della , Morelli Giancarlo , Vitagliano Luigi , Velotta Raffaele , Accardo Antonella 
Published: August 2021

Abstract
The self-assembling of small peptides not only leads to the formation of intriguing nanoarchitectures, but also generates materials with unexpected functional properties. Oligopeptides can form amyloid-like cross-β assemblies that are able to emit intrinsic photoluminescence (PL), over the whole near-UV/visible range, whose origin is still largely debated. As proton transfer between the peptide chain termini within the assembly is one of the invoked interpretations of this phenomenon, we here evaluated the solid state PL properties of a series of self-assembled hexaphenalanine peptides characterized by a different terminal charge state. Overall, our data indicate that the charge state of these peptides has a marginal role in the PL emission as all systems exhibit very similar multicolour PL associated with a violation of the Kasha’s rule. On the other hand, charged/uncharged ends occasionally produce differences in the quantum yields. The generality of these observations has been proven by extending these analyses to the Aβ16−21 peptide. Collectively, the present findings provide useful information for deciphering the code that links the spectroscopic properties of these assemblies to their structural/electronic features.