bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anesthesia-Related Airway Patient Safety Incidents Correlated with Major Adverse Cardiac Events: A Single-Center Retrospective Case-Control Analysis from 2009 to 2019
Authors: Zhang Xue , Wu Lingeer , Huang Huizhen , Zhang Yuelun , Shen Le , Huang Yuguang 
Published: August 2021

Abstract
Background: Airway-related patient safety incidents (PSIs) have always been the top concern of anesthesiologists because this type of incident could severely threaten patient safety if not treated immediately and properly. This study aimed to reveal the composition and outcomes of and to identify risk factors related to airway incidents reported by anesthesiologists.Methods: All airway-related PSIs reported by anesthesiologists at a Chinese academic hospital between September 2009 and August 2019 were collected from the PSI reporting system. Patients with reported airway incidents were matched 1:1 with controls based on sex and type of surgery. Univariate and multivariate analyses were performed to identify risk factors associated with airway incident occurrence and to evaluate the influence of airway PSIs on the prognosis.Results: Among 773 PSIs voluntarily reported by anesthesiologists during the study period, 208 (26.9%) were airway-related incidents, with an overall reporting incidence of 4.59 per 10,000. In patients with reported airway PSIs, postoperative major adverse cardiac events (MACEs) were more likely (7 vs 1 case, odds ratio: 7.37 [95% confidence interval (CI): 0.89 to 60.83], p=0.031) and the extubation time (0.82±1.63 d vs 0.21±0.89 d, mean difference: 0.61 d [95% CI: 0.28 to 0.95 d], p<0.001) and ICU length of stay (LOS) (2.03±6.66 d vs 0.22±0.96 d, mean difference 1.81 d [95% CI: 0.60 to 3.02 d], p=0.004) were longer. Only the American Society of Anesthesiologists (ASA) physical status was found to be a significant independent predictor of these airway PSIs.Conclusions: This single-institutional retrospective case-control study describes the composition of airway-related PSIs reported by anesthesiologists within eleven years. Airway incidents influence the prognosis by increasing the incidence of postoperative MACEs and prolonging both the extubation time and ICU LOS. Airway PSI data are worth analyzing to improve patient safety.
Efficient Loading of Mano Mn Particles On Calcined Laterite Soil (Lt-nMn) For Higher Removal of As(III) Ions From Groundwater: Adsorption And Eco-Scale Analysis
Authors: Prabhakar Roshan , Ghosh Somaparna , Malik Ali , Samadder Sukha Ranjan 
Published: August 2021

Abstract
Occurrence of arsenic in the groundwater has become a cause for concern in many countries. The presence of As(III) species in the groundwater had been one of the biggest challenges for the water workers especially in the south east Asian countries. Nano based adsorption techniques are gaining attention among researchers for the removal of arsenic ions. However, synthesis of nano-adsorbents is a costly affair. Therefore, the present study utilized the cheap and readily available laterite soil as a base material for nano coating. Nano sized manganese (nMn) particles were synthesized by chemical reduction method and later coated on calcined laterite (Lt-nMn). Coating with 0.1 M nMn provided the best As(III) removal efficiency. The prepared material was characterised for its morphological and surface properties. Phase analysis using XRD (X-ray diffraction) showed the presence of zero valent manganese species, which assisted in adsorption. Adsorption studies were conceded by selecting the different affecting parameters such as contact time, concentration, dose, temperature, and pH. Very high removal in less time regime led to the investigation of the oxidation mechanism. Phosphate and sulphate anions insignificantly reduced the removal efficiency. Langmuir model for the sorption isotherms and pseudo-second order kinetic model for the sorption kinetics symbolized the experimental data. Sustainability studies in terms of eco-scaling and cost analysis were performed for the applied method of production of the nano materials. Adsorber design analysis was also conducted to estimate the required amount of Lt-nMn particles for achieving the desired equilibrium As(III) concentration.
Screening The Efficacy of Melatonin On Neurodegeneration Mediated By ER Stress, Inflammation And Oxidative Damage
Authors: Basha Fathima Hajee , Hemalatha S. 
Published: August 2021

Abstract
Neurodegeneration may be defined as a clinical condition wherein neurons gradually lose their structural integrity, viability, functional abilities and the damage inflicted upon the neurons is often irreversible. The number of elderly patients suffering from Neurodegenerative disorders is expected to rise tremendously over the next couple of years. Thus, there is an urgent need to delve into and study the underlying cause and mechanisms, so that we may be able to develop more effective therapeutic strategies and drugs and better understand the origin and progression of the disease.The various mechanisms that have been observed to contribute to neurodegeneration include aggregation and accumulation of misfolded proteins, impaired autophagy, oxidative damage, neuroinflammation, mitochondrial defects, increased SUMOylation of proteins, impaired UPR pathways, disruption of axonal transport.Melatonin, a neurohormone is involved in a variety of functions including scavenging free radicals, synchronizing the circadian rhythm, mitigating immune response.Melatonin has shown to modulate the UPR pathway ,antioxidant pathway through Nrf2 and inflammatory pathway through NFκB. The study aims to determine the efficacy of melatonin on neurodegeneration mediated by ER stress, inflammation and oxidative damage through in silico approaches. The molecular targets chosen were ATF6, XBP1, PERK, Nrf2, NFκB and they were docked against melatonin. Additionally various physiochemical analysis such as ADME were also carried out to determine its drug ability. The findings were that melatonin not only shows excellent interactions with the targets but also possess drug-like physicochemical properties that makes it a valuable choice for the treatment of neurodegenerative disorders.
Aldolase A Promotes Proliferation and Metastasis of Colorectal Cancer through Targeting COPS6 and Regulating MAPK Signaling Pathway
Authors: Lu Ya , Zhang Yuan , Zhang Hui , Zhu Yue , Zhang Junying , Sha Huanhuan , Zou Renrui , Gan Yujie , Wu Jianzhong , Feng Jifeng 
Published: August 2021

Abstract
Background: Colorectal cancer (CRC) is a serious threat to human health, and its underlying mechanisms needs further explored. Aldolase A (ALDOA) has received increasing attention for its reported association with multiple cancers, but the function and mechanism of ALDOA in CRC remain unclear. We aimed to evaluate the biological role of ALDOA in CRC.Methods: The stable ALDOA knockdown or overexpression cell lines were established for subsequent experiments. The qRT-PCR and western blotting were used to detect the expression of ALDOA and COPS6 and the relative protein levels of epithelial-mesenchymal transition (EMT) and MAPK signaling pathway. Immunofluorescence (IF) assay was applied to determine ALDOA localization. CCK-8, transwell, and wound healing assays were performed to evaluate CRC cell proliferation, invasion, and migration. Mouse xenograft models were established to verify the effect of ALDOA on CRC cell growth in vivo. Immunoprecipitation (IP) assay and mass spectrometry (MS) analysis were conducted to identify the interactions between ALDOA and COPS6.Results: ALDOA was overexpressed in CRC tissues and cell lines. Silencing ALDOA significantly impaired the proliferation, invasion and migration of CRC cells in vitro, and obviously decreased the growth of CRC cells in vivo. Mechanically, ALDOA bound to and regulated COPS6, and the promoting effects of upregulated ALDOA on CRC cell proliferation and metastasis were inhibited by the depletion of COPS6. Besides, EMT program and MAPK signaling pathway were activated by ALDOA overexpression.Conclusion: ALDOA facilitated the proliferation, invasion and migration of CRC through binding and regulating COPS6, inducing EMT and activating MAPK signaling pathway.
Melatonin Protects TEGDMA induced Pre-odontoblasts Mitochondrial Apoptosis via JNK/MAPK Signaling Pathway
Authors: Yu Qihao , Liu Yi , Wang Konghuai , Weng Xunben , Huang Shengbin , Pan Yihuai 
Published: August 2021

Abstract
Background: Resin monomer induced dental pulp injury presents a mitochondrial dysfunction related pathology. Melatonin has been regarded as a strong mitochondrial protective bioactive compound from pineal gland. However, it remains unknown whether melatonin can prevent dental pulp from resin monomer induced injury. The aim of the study is to investigate the effects of melatonin on TEGDMA, a major component in dental resin, induced mouse pre-odontoblast cell lines (mDPC6T) mitochondrial apoptosis and to verify whether JNK/MAPK signaling pathway mediate the protective effect of melatonin. Methods: We adopted a well-established TEGDMA-induced mDPC6T apoptosis model to investigate the preventive effect of melatonin by detecting cell viability, apoptosis rate, expression of apoptosis related protein, mitochondrial ROS (mtROS) production, mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) level. Inhibitors of MAPKs signaling were used to explore which pathway was participated in TEGDMA induced apoptosis. Finally, we verified the role of JNK/MAPK pathway during the protective effects of melatonin above by the agonist and antagonists of JNK.Results: Melatonin attenuated TEGDMA induced mDPC6T apoptosis via reducing mtROS production, rescuing MMP and ATP level. Meanwhile, the mitochondrial dysfunction and apoptosis was alleviated by the JNK/MAPK inhibitor SP600125 but not the other MAPKs signaling inhibitors. Furthermore, melatonin down-regulated the expression of phosphorylated-JNK, and eliminated the active effects of Anisomycin on JNK/MAPK pathway, which mimicked the effects of the SP600125.Conclusion: Our findings demonstrated that melatonin protected mDPC6T against TEGDMA induced apoptosis via JNK/MAPK signaling and maintenance of mitochondrial function, which presented a novel therapeutic strategy for prevention against resin monomer-induced dental pulp injury.
Using Virtual Reality in Lumbar Puncture Training Improves Students Learning Experience
Authors: Vrillon Agathe , Gonzales-Marabal laurent , Ceccaldi Pierre-François , Plaisance Patrick , Desrentes Eric , Paquet Claire , Dumurgier Julien 
Published: August 2021

Abstract
Background Lumbar puncture (LP) is a commonly performed medical procedure in a wide range of indications. Virtual reality (VR) provides a stimulating, safe and efficient learning environment. We report the design and the evaluation of a three dimensions (3D) video training for LP procedure.MethodsWe recorded a stereoscopic 180-degrees 3D video from two LPs performed in clinical setting in Fernand Widal Lariboisière University Hospital, Paris, France. Video was administrated to third-year medical students as well as to a residents and attendings group during LP simulation-based training sessions. ResultsOn 168 participants (108 novice third-year medical students, and 60 residents and attendings with prior LP experience), satisfaction after video exposure was high (rated 4.7 ± 0.6 on a 5-point scale). No significant discomfort was reported (comfort score graded 4.5 ± 0.8 on 5). LP-naive students displayed higher satisfaction and perceived benefit than participants with prior LP experience (overall, P < 0.05). Trainees evaluated favorably the 3D feature and supported the development of similar supports for other medical procedures (respectively, 3.9 ± 1.1 and 4.4 ± 0.9 on 5).ConclusionWe report our experience with a 3D video for LP training. VR support could increase knowledge retention and skills acquisition in association to LP simulation training.
Phytotoxicity of atrazine combined with cadmium on photosynthetic apparatus of the emergent plant species Iris pseudacorus
Authors: Wang Qinghai , Xie Dongyu , Peng Lei , Chen Chuansheng , Li Cui , Que Xiaoe 
Published: August 2021

Abstract
The combined pollution, instead of single pollution, has become a widespread contamination phenomenon in aquatic environment. However, little information is now available about the joint effects of the combined pollution, especially co-existed pesticides and heavy metals, on aquatic plants. In the present study, using continuous excitation chlorophyll fluorescence parameters and the OJIP transient, comparisons of herbicide atrazine (ATZ) phytotoxicity on Iris pseudacorus between in the presence and absence of cadmium (Cd) were evaluated over an exposure period of three weeks under laboratory conditions. Results showed that both ATZ and Cd were toxic to I. pseudacorus. The ratio Fv/Fo, specific electron transport energy (ET0/RC) and photochemistry efficiency (PIabs and PItotal) of this emergent plant species at individual ATZ and Cd concentrations were significantly lower than those of the control. ATZ mainly inhibited electron transport beyond QA at PSII acceptor side as indicated by the sharp rise of the J-step level of fluorescence rise kinetics. A pronounced K-step and the loss of I-step due to the damage on the OEC and PSI also occurred when ATZ was at or above 1.0 mg·L− 1. In comparison to ATZ alone, ATZ combined with Cd resulted in a lower amplitude rise in J-step with apparent J-I and I-P phases; and significantly lower Fo with higher Fv/Fo, as well as greater ET0/RC with higher values of PIabs and PItotal. However, the adverse influences of ATZ combined with Cd on the above indicators were still significant as compared with the control. Therefore, the coexistence of Cd alleviated the individual phytotoxicities of ATZ, whereas combined pollution of ATZ and Cd still induced the decline in photosynthetic performance of I. pseudacorus, and its potential ecological impacts on the aquatic vegetation cannot be ignored. Our findings offer a better understanding of the joint effects of the pesticide and heavy metal on non-target aquatic plants, and provided valuable insights into the interaction of these pollutants in aquatic environment.
Immunogenicity of heterologous prime/booster-inactivated and adenoviral-vectored COVID-19 vaccine: real-world data
Authors: Wanlapakorn Nasamon , Suntronwong Nungruthai , Phowatthanasathian Harit , Yorsang Ritthideach , Thongmee Thanunrat , Vichaiwattana Preeyaporn , Auphimai Chompoonut , Wongsrisang Lakkhana , Klinfueng Sirapa , Sudhinaraset Natthinee , Thanasitthichai Somchai , Akksilp Somsak , Poovorawan Yong 
Published: August 2021

Abstract
Limited data are available on the responses to heterologous vaccine regimens for SARS-CoV-2, especially among countries using inactivated and adenoviral-vectored vaccines. A total of 77 participants who received heterologous prime/booster-inactivated COVID-19 vaccine and adenoviral-vectored vaccine were enrolled in our study. There were two comparison groups vaccinated with the homologous CoronaVac (N = 79) and AZD1222 (N = 80) regimen. All sera samples were tested for SARS-CoV-2 spike receptor-binding-domain (RBD) IgG using a chemiluminescent microparticle immunoassay (CMIA). The neutralizing activity in a subset of serum samples was tested against the original Wuhan strain and variants of concern, B.1.1.7 and B.1.351, using an enzyme-linked immunosorbent assay (ELISA)-based surrogate virus neutralization test (sVNT). The CoronaVac followed by the AZD1222 vaccine induced higher levels of spike RBD-specific IgG than that of two-dose CoronaVac or AZD1222 vaccines (p < 0.001). Sera samples of the CoronaVac/AZD1222 vaccine recipients elicited higher neutralizing antibody activity against the original Wuhan and the B.1.351 strain than in the recipients of the two-dose CoronaVac or AZD1222. Following the inactivated CoronaVac/adenoviral-vectored (AZD1222) vaccination administered 14–72 days apart, participants receiving the heterologous vaccine regimen had higher spike RBD-specific IgG and neutralizing activities than the homologous CoronaVac vaccine recipients.
Tissue-specific H2Bub1 is required for cardiomyocyte development
Authors: Barish Syndi , Drozd Jeffrey , Berg Kathryn , Berglund-Brown Isabella , Khizir Labeeqa , Wasson Lauren , Chen Sidi , Brueckner Martina 
Published: August 2021

Abstract
De-novo mutations affecting Histone2B-K120 monoubiquitination (H2Bub1) are enriched in congenital heart disease; however, how H2Bub1 affects heart development beyond its function in establishing left-right asymmetry remains unknown. Here we show that the RNF20-core complex (RNF20-RNF40-UBE2B), which catalyzes H2Bub1, is required for cardiac development in-vivo in mice and in-vitro in iPSC-derived cardiomyocytes. Mice with cardiac-specific deletion of Rnf20 have abnormal myocardium and ventricular septal defects; iPSCs with mutations affecting H2Bub1 cannot efficiently differentiate into cardiomyocytes. Sarcomeric gene expression is dependent on normal H2Bub1 both in mice and in iPSC-derived cardiomyocytes. Finally, we identify an accumulation of H2Bub1 near the center of tissue-specific genes in cardiomyocytes and MEFs associated with transcriptional efficiency that is reduced in UBE2B-/- cardiomyocytes. In summary, normal H2Bub1 distribution is required in cardiac development, and H2Bub1 accumulation acts as a general mechanism for tissue-specific regulation of transcriptional elongation efficiency.
Occurrences, Distribution and Composition of Microplastics in the Surface Seawater and Beach Sediments of the Southern Shandong Peninsula, China
Authors: Pervez Rashid , Wang Yonghong , Lai Zhongpeng 
Published: August 2021

Abstract
The current work provides the detailed analysis about microplastics contamination of the beach sediments and surface seawater. Sediment samples were collected from the high tidal zone at 13 beaches, and surface seawaters of seven stations along the southern Shandong Peninsula. The presence of microplastics was recorded on both beaches and surface seawater. The mean quantity of microplastics was approximately 664±80 microplastics/kg of dry weight (dw) with 0.5~0.125 mm in beach sediments, and 5.62±0.4 microplastics/liter (L) of surface seawater. Predominant microplastics were fibers (>97%). While other plastic forms had lower percentage. Microplastics possessed different color with polymer composition including polypropylene, polyethylene, and polystyrene. The accumulation of microplastics on beaches is an alarming issue and provides better understanding about microplastics pollution along the coastal regions of the southern Shandong Peninsula. It would also help to establish better monitoring system for future and evaluate possible risks by microplastics due to rapid economic growth of China.
YY1 Activates EMI2 and Promotes the Progression of Cholangiocarcinoma Through the PI3K/Akt Signaling Axis
Authors: zhou Shuai , Qu Kang Lin , Li JinAng , Chen Shilei , Zhang Yi Gang , Zhu Chao , Jin Hao , Wang yong , Pang Qing , Liu Hui Chun 
Published: August 2021

Abstract
Background: Cholangiocarcinoma (CCA) is one of the deadliest cancers of the digestive tract. The prognosis of CCA is poor and the 5-year survival rate is low. Bioinformatic analysis showed that early mitotic inhibitor 2 (EMI2) was overexpressed in CCA but the underlying mechanism is not known.Methods: The data on bile duct carcinoma from TCGA and GEO databases were used to detect the expression of EMI2. The transcription factors of EMI2 were predicted using JASPAR and PROMO databases. Among the predicted transcription factors, YY1 has been rarely reported in cholangiocarcinoma, and was verified using the luciferase reporter gene assay. RT-PCR was performed to predict the downstream pathway of EMI2, and PI3K/Akt was suspected to be associated with it. Subsequently, in vivo and in vitro experiments were conducted to verify the effects of silencing and overexpressing EMI2 and YY1 on the proliferation, invasion, and metastasis of the bile duct cancer cells.Results: EMI2 was highly expressed in CCA. Silencing EMI2 inhibited the proliferation, invasion, and migration of CCA cells, arrested cell cycle in the G1 phase, and inhibition of apoptosis. The luciferase reporter gene assay showed that YY1 bound to the promoter region of EMI2, and after silencing YY1, the expression of EMI2 decreased and the progression of CCA was inhibited. Moreover, key proteins in the PI3K/Akt signaling pathway decreased after silencing EMI2.Conclusion: EMI2 may be one of the direct targets of YY1 and promotes the progression of CCA through the PI3K/Akt signaling pathway.
Investigation of Dissimilatory Nitrate Reduction to Ammonium (DNRA) Microbial Communities in Sediments of Urban River Network Along the Huangpu River, china
Authors: Zhang Tong , Zhuang Xuming , Ahmad Shakeel , Lee Taeho , Cao Chengbo , Ni Shou-Qing 
Published: August 2021

Abstract
Dissimilatory nitrate reduction to ammonium (DNRA) is an essential intermediate step in the nitrogen cycle, linking the oxidation and reduction processes of nitrogen compounds. But the detailed research on the environmental nitrogen cycling in urban river networks based on DNRA communities and the functional gene nrfA is lacking. In this study, the flow line of the Huangpu River in Shanghai was analyzed using isotope tracer, quantitative real-time PCR, and high-throughput sequencing techniques to evaluate the role of DNRA on the stability of the river network and marine. The significant positive correlation between the rate of DNRA and sediment organic carbon was identified. At the genus level, Anaeromyxobacter is the most dominant. Notably, both heterotrophic and autotrophic DNRA species were discovered. This study added diversity to the scope of urban freshwater river network ecosystem studies by investigating the distribution of DNRA bacteria along the Huangpu River.
Rotating Object Detection in Remote-Sensing Environment
Authors: Chan Sixian , Zheng Jingcheng , Wang Lina , Wang Tingting , Zhou Xiaolong , Xu Yingkun , Fang Kai 
Published: August 2021

Abstract
Deep learning models have become the mainstream algorithm for processing computer vision tasks. In object detection tasks, the detection box is usually set as a rectangular box aligned with the coordinate axis, so as to achieve the complete package of the object. However, when facing some objects with large aspect ratio and angle, the bounding box has to become large, which makes the bounding box contain a large amount of useless background information. In this study, a different approach is taken, using a method based on YOLOv5, the angle information dimension is increased in head part and angle regression added at the same time of the border regression, combining ciou and smoothl1 to calculate the bounding box loss, so that the resulting border box fits the actual object more closely. At the same time, the original dataset tags are also preprocessed to calculate the angle information of interest. The purpose of these improvements is to realize object detection with angles in remote-sensing images, especially for objects with large aspect ratios, such as ships, airplanes, and automobiles. Compared with the traditional object detection model based on deep learning, experimental results show that the proposed method has a unique effect in detecting rotating objects.