bluuebunny/crossref_metadata_2025_split huggingface dataset:
Territorial damselflies are larger and show negative allometry in their genitalia
Authors: Galicia-Mendoza Dalia Ivette , Sanmartín-Villar Iago , García-Miranda Óscar , Cordero-Rivera Adolfo 
Published: July 2021

Abstract
The ‘functional allometry’ hypothesis proposes that the variation in allometric patterns of sexually selected traits is related to their function. We hypothesize that the allometric patterns for genitalia of aggressively territorial organisms are different from those in non-territorial organisms and predict that in aggressively territorial species, where body size is related directly to reproductive success, males must allocate more resources to body size than to genitalia. We studied 59 species of damselflies in 51 genera. Species were divided into three categories: highly territorial and aggressive; low aggressive; and not aggressive. We measured the length of the genital ligula, the width at the basis and its maximum width, and we used body length and wing length as descriptors of body size. The slope of allometric relationships was estimated using ordinary least squares and reduced major axis regressions. Our results indicated first, that territorial damselflies are larger and that body length and wing length are not equivalent as estimators of body size in odonates. Second, ordinary least squares and reduced major axis regressions provided different results in some of the analyses. Third, we found that aggressive species have less steep allometric slopes than non-aggressive species, both for the length of the ligula and for the width at its basis.
Functional study of distinct domains of Dux in improving mouse SCNT embryonic development†
Authors: Huang Xingwei , Hu Xinglin , Jiang Qi , Cao Qianzi , Wu Yanshuang , Lei Lei 
Published: July 2021

AbstractTwo-cell-like (2C-like) embryonic stem cells (ESCs) are a small group of ESCs that spontaneously express zygotic genome activation (ZGA) genes and repeats, such as Zscan4 and murine endogenous retrovirus with leucine (MERVL), and are specifically expressed in 2-cell-stage mouse embryos. Although numerous types of treatment and agents elevate the transition of ESCs to 2C-like ESCs, Dux serves as a critical factor in this transition by increasing the expression of Zscan4 and MERVL directly. However, the loss of Dux did not impair the birth of mice, suggesting that Dux may not be the primary transitioning factor in fertilized embryos. It has been reported that for 2-cell embryos derived from somatic cell nuclear transfer (SCNT) and whose expression of ZGA genes and repeats was aberrant, Dux improved the reprogramming efficiency by correcting aberrant H3K9ac modification via its C-terminal domain. We confirmed that the overexpression of full-length Dux mRNA in SCNT embryos improved the efficiency of preimplantation development (62.16% vs. 41.26% with respect to controls) and also increased the expression of Zscan4 and MERVL. Furthermore, we found that the N-terminal double homeodomains of Dux were indispensable for Dux localization and function. The intermediate region was essential for MERVL and Zscan4 activation, and the C-terminal domain was important for elevating level of H3K27ac. Mutant Dux mRNA containing N-terminal double homeodomains with the intermediate region or the C-terminal domain also improved the preimplantation development of SCNT embryos. This is the first report focusing on distinguishing functional domains of Dux in embryos derived from SCNT.
Hypoxia-induced alteration of RNA modifications in the mouse testis and sperm
Authors: He Tong , Guo Huanping , Shen Xipeng , Wu Xiao , Xia Lin , Jiang Xuelin , Xu Yinying , Chen Dan , Zhang Yunfang , Tan Dongmei , Tan Yi 
Published: July 2021

Abstract
Hypobaric hypoxia as an extreme environment in a plateau may have deleterious effects on human health. Studies have indicated that rush entry into a plateau may reduce male fertility and manifest in decreased sperm counts and weakened sperm motility. RNA modifications are sensitive to environmental changes and have recently emerged as novel post-transcriptional regulators in male spermatogenesis and intergenerational epigenetic inheritance. In the present study, we generated a mouse hypoxia model simulating the environment of 5500 m in altitude for 35 days, which led to compromised spermatogenesis, decreased sperm counts, and an increased sperm deformation rate. Using this hypoxia model, we further applied our recently developed high-throughput RNA modification quantification platform based on liquid chromatography with tandem mass spectrometry, which exhibited the capacity to simultaneously examine 25 types of RNA modifications. Our results revealed an altered sperm RNA modifications signature in the testis (6 types) and mature sperm (11 types) under the hypoxia model, with 4 types showing overlap (Am, Gm, m7G, and m22G). Our data first drew the signature of RNA modification profiles and comprehensively analyzed the alteration of RNA modification levels in mouse testis and sperm under a mouse hypoxia model. These data may be highly related to human conditions under a similar hypoxia environment.
Endovascular repair of descending thoracic aortic aneurysms—a mid-term report from the Global Registry for Endovascular Aortic Treatment (GREAT)
Authors: Grassi Viviana , Trimarchi Santi , Weaver Fred , de Beaufort Hector W L , Azzizzadeh Ali , Upchurch Jr Gilbert R , Piffaretti Gabriele , Lomazzi Chiara ,   the GREAT participants
Published: July 2021

Abstract

OBJECTIVES
The aim of this study was to evaluate the short- to mid-term outcomes of descending thoracic aortic aneurysm (DTAA) repair from the Gore Global Registry for Endovascular Aortic Treatment (GREAT).

METHODS
This is a multicentre sponsored prospective observational cohort registry. The study population comprised those treated for DTAA receiving GORE thoracic aortic devices for DTAA repair between August 2010 and October 2016. Major primary outcomes were early and late survival, freedom from aorta-related mortality and freedom from aorta-related reintervention.

RESULTS
There were 180 (58.1%) males and 130 (41.9%) females: the mean age was 70 ± 11 years (range 18–92). The median maximum DTAA diameter was 60 mm (interquartile range 54–68.8). Technical success was achieved in all patients. Operative mortality, as well as immediate conversion to open repair, was never observed. At the 30-day window, mortality occurred in 4 (1.3%) patients, neurological events occurred in 4 (1.3%) patients (transient ischaemic attacks/stroke n = 3, paraplegia n = 1) and the reintervention rate was 4.5% (n = 14). Estimated survival was 95.6% [95% confidence interval (CI) 92.6–97.4] at 6 months, 92.7% (95% CI 89.1–95.2) at 1 year and 57.3% (95% CI 48.5–65.1) at 5 years. Freedom from aorta-related mortality was 98.3% (95% CI 96.1–99.3) at 6 months, 98.3% (95% CI 96.1–99.3) at 1 year and 92.2% (95% CI 83.4–96.4) at 5 years. Freedom from thoracic endovascular aortic repair (TEVAR)-related reintervention at 5 years was 87.2% (95% CI 81.2–91.4).

CONCLUSIONS
TEVAR for DTAAs using GORE thoracic aortic devices is associated with a low rate of device-related reinterventions and is effective at preventing aorta-related mortality for up to 5 years of follow-up.

Clinical registration number
NCT number: NCT01658787.

Subject collection
161, 164.
How to minimize the circulatory arrest time by using the Thoraflex Hybrid prosthesis: the ‘release and perfuse’ technique
Authors: Piperata Antonio , d’Ostrevy Nicola , Busuttil Olivier , Pernot Mathieu 
Published: July 2021

Abstract
Although the frozen elephant trunk technique permits a complete single-stage treatment in patients with extended thoracic aortic disease, the problem of circulatory arrest time remains unsolved. We propose a simplified use of the Thoraflex Hybrid prosthesis to minimize the circulatory arrest time.
Next-generation sequencing to confirm clinical familial hypercholesterolemia
Authors: Reeskamp Laurens F , Tromp Tycho R , Defesche Joep C , Grefhorst Aldo , Stroes Erik S G , Hovingh G Kees , Zuurbier Linda 
Published: July 2021

Abstract

Background
Familial hypercholesterolemia is characterised by high low-density lipoprotein-cholesterol levels and is caused by a pathogenic variant in LDLR, APOB or PCSK9. We investigated which proportion of suspected familial hypercholesterolemia patients was genetically confirmed, and whether this has changed over the past 20 years in The Netherlands.

Methods
Targeted next-generation sequencing of 27 genes involved in lipid metabolism was performed in patients with low-density lipoprotein-cholesterol levels greater than 5 mmol/L who were referred to our centre between May 2016 and July 2018. The proportion of patients carrying likely pathogenic or pathogenic variants in LDLR, APOB or PCSK9, or the minor familial hypercholesterolemia genes LDLRAP1, ABCG5, ABCG8, LIPA and APOE were investigated. This was compared with the yield of Sanger sequencing between 1999 and 2016.

Results
A total of 227 out of the 1528 referred patients (14.9%) were heterozygous carriers of a pathogenic variant in LDLR (80.2%), APOB (14.5%) or PCSK9 (5.3%). More than 50% of patients with a Dutch Lipid Clinic Network score of ‘probable’ or ‘definite’ familial hypercholesterolemia were familial hypercholesterolemia mutation-positive; 4.8% of the familial hypercholesterolemia mutation-negative patients carried a variant in one of the minor familial hypercholesterolemia genes. The mutation detection rate has decreased over the past two decades, especially in younger patients in which it dropped from 45% in 1999 to 30% in 2018.

Conclusions
A rare pathogenic variant in LDLR, APOB or PCSK9 was identified in 14.9% of suspected familial hypercholesterolemia patients and this rate has decreased in the past two decades. Stringent use of clinical criteria algorithms is warranted to increase this yield. Variants in the minor familial hypercholesterolemia genes provide a possible explanation for the familial hypercholesterolemia phenotype in a minority of patients.
The Scattering: a Family History for a Floating World
Authors: Moran Joe 
Published: July 2021

Abstract
This essay is an experiment in family history, inspired by a journey to scatter my father’s ashes on Scattery – the island in the Shannon estuary where my grandmother was born and raised. It explores how my family’s story illuminates the history of small Irish islands and of two much bigger islands, Ireland and Britain. Island stories replay in microcosm, and with great intensity, broader narratives of Irish and British history since the famine. But they also muddy these narratives with the idiosyncratic, granular experiences of a precise and bounded place. They put human flesh and bones on abstract nouns like family, community, modernity, emigration and exile. In every island story – Scattery as my father’s idyllic land of endless summer, Ireland as the home of sturdy children and happy maidens, Britain as proud island nation taking back control of its borders – lies a fantasy of homogeneity and readability. Even the smallest islands have multilayered histories; and then, as they disperse their peoples across the world as small islands do, those histories keep multiplying. Our journey back to the island may have begun as a search for origins but it ended up revealing the heterogeneity and unrecoverability of the past.
Inhibition of d-alanylation of teichoic acids overcomes resistance of methicillin-resistant Staphylococcus aureus
Authors: Coupri Delphine , Verneuil Nicolas , Hartke Axel , Liebaut Axelle , Lequeux Thierry , Pfund Emmanuel , Budin-Verneuil Aurélie 
Published: July 2021

Abstract

Background
MRSA are high-priority multidrug-resistant pathogens. Although there are still some antibiotics active against MRSA, continuous efforts to discover new antibiotics and treatment strategies are needed because resistance to these new drugs has already been reported.

Objectives
Here we explore if d-alanylation of teichoic acids (TAs) mediated by the dlt operon gene products might be a druggable target to overcome β-lactam-resistance of MRSA.

Methods
MICs and bactericidal effects of several β-lactam antibiotics were monitored in a panel of clinical MRSA strains with genetic or chemically induced deficiency in d-alanylation of TAs. Efficiency of the chemical inhibitor to rescue MRSA-infected larvae of Galleria mellonella as well as its ability to prevent or eradicate biofilms of S. aureus were analysed.

Results
Genetic inactivation of the Dlt system or its chemical inhibition re-sensitizes MRSA to β-lactams. Among the 13 strains, the most pronounced effect was obtained using the inhibitor with imipenem, reducing the median MIC from 16 to 0.25 mg/L. This combination was also bactericidal in some strains and significantly protected G. mellonella larvae from lethal MRSA infections. Finally, inactivation of d-alanylation potentiated the effect of imipenem on inhibition and/or eradication of biofilm.

Conclusions
Our combined results show that highly efficient inhibitors of d-alanylation of TAs targeting enzymes of the Dlt system should be promising therapeutic adjuvants, especially in combination with carbapenems, for restoring the therapeutic efficacy of this class of antibiotics against MRSA.
Fentanyl, Acetylfentanyl and Carfentanil in Impaired Driving Cases: A Review of 270 Cases
Authors: Kiely Elizabeth , Juhascik Matthew 
Published: July 2021

Abstract
The detection of fentanyl (FEN) and FEN analogs has been widely communicated throughout the scientific community. While most of the reporting has been in relation to overdose deaths, these drugs are commonly detected in impaired driving cases. A retrospective study of impaired driving cases analyzed between 2017 and 2019 produced 270 cases positive for FEN, carfentanil (CFEN) and/or acetylfentanyl (AFEN). FEN was the predominant drug found in these 270 cases (65.5%) with concentrations ranging from less than 1.0 to 64 ng/mL. CFEN was found alone in 6.6% cases with three concentrations above 1.0 ng/mL. AFEN was always found when FEN was positive with concentrations ranging from &lt;1.0 to 9.2 ng/mL. Detailed case histories are provided with corresponding toxicology results. Toxicology results show impaired drivers using multiple drugs with a wide range of observed behaviors. The inclusion of these drugs in routine impaired driver toxicology testing is extremely important when attempting to determine their overall prevalence.
Novel approach to removing a steel bar extending from the scrotum to anterior abdominal wall: a case report
Authors: Wen Chen-Yueh , Ho Kai-Hung , Wu Meng-Yu , Lin Jen-Tai , Yu Chia-Cheng , Chen Yin-Shen 
Published: July 2021

Abstract
Foreign body penetration from the scrotum is not rare. If a large and sharp foreign body pierces through an existing wound, it likely must be extended to improve exposure. Therefore, many traumatologists attempt to minimize the surgical wound caused by the removal of the foreign body. We describe a case of a foreign object penetrating from the scrotum to anterior abdominal wall. Our novel surgical approach is safe and feasible to use and provides minimal scarring, thereby allowing rapid recovery.
Enhancing Partner Nation Security: A Mixed-Methods Evaluation of A Peacekeeping Public Health Capacity Building Initiative
Authors: Snowden Bobbi S , Licina Derek , Crowley Kathleen , Migliaccio Gene , Ramsey Sueann 
Published: July 2021

ABSTRACT

Introduction
One way the U.S. Department of Defense (DoD) works to achieve national security is through security cooperation, by way of building and enhancing partner nation capacity. This study evaluated a health-related security cooperation training initiative delivered by the DoD to military peacekeepers. The study specifically examined outcomes of change, including the beginning phase of sustainability.

Materials and Methods
The U.S. DoD employed a train-the-trainer model in Ghana, Rwanda, Senegal, and Uganda to support the African Peacekeeping Rapid Response Partnership program. U.S. instructors trained 192 peacekeepers through 11 training iterations between December 2016 and March 2020. A mixed-method explanatory sequential design was used to explore training outcomes. Quantitatively, three hypotheses were tested using nonparametric statistical analysis. Qualitative analysis of documents was used to inform and contextually understand the quantitative results. This study was submitted to the George Washington University Institutional Review Board and was fully approved (NCR202918).

Results
Quantitative and qualitative results indicated improved short-term public health knowledge and upskill among partner nation participants. There was the beginning of a cascade effect of the partner nations’ ability to autonomously teach tasks and skills to their military to sustain the initiative. Differences in achieving and maintaining change outcomes were related to student characteristics, the training course, and the partner nation.

Conclusions
This research serves as the first published study to empirically examine health-related security cooperation train-the-trainer initiative change outcomes. This research is an essential building block to empirically evaluate and capture change outcomes from security cooperation capacity building training initiatives. The findings and recommendations inform security cooperation policy and associated investments.
Army Urban Augmentation Medical Task Forces for COVID-19: Part 1—Development
Authors: Dire Daniel J , Suter Robert E , Robinson Joe D , Lynn W Scott 
Published: July 2021

ABSTRACT
This article describes how the U.S. Army developed a new ad hoc medical formation, named Urban Augmentation Medical Task Force for the Department of Defense (DoD) in response to the Coronavirus Disease 2019 pandemic in the Continental United States during the spring of 2020. We review the role of the DoD support of the Federal Emergency Management Agency as a part of Defense Support of Civilian Authorities.
Generating neutron-star magnetic fields: three dynamo phases
Authors: Lander S K 
Published: July 2021

ABSTRACTYoung neutron stars (NSs) have magnetic fields in the range 1012–1015 G, believed to be generated by dynamo action at birth. We argue that such a dynamo is actually too inefficient to explain the strongest of these fields. Dynamo action in the mature star is also unlikely. Instead we propose a promising new precession-driven dynamo and examine its basic properties, as well as arguing for a revised mean-field approach to NS dynamos. The precession-driven dynamo could also play a role in field generation in main-sequence stars.
Midterm Outcomes After Low-Profile Visualization Endoluminal Support or Atlas Stent-Assisted Coiling of Intracranial Aneurysms: A Propensity Score Matching Analysis
Authors: Yeon Eung Koo , Cho Young Dae , Yoo Dong Hyun , Kim Jeong Eun , Kim Kang Min , Lee Sung Ho , Cho Won-Sang , Kang Hyun-Seung 
Published: July 2021

Abstract

BACKGROUND
The interplay of various commercially available stents during coil embolization of intracranial aneurysms and their ultimate impact are subject to debate.

OBJECTIVE
To compare midterm outcomes of Low-Profile Visualization Endoluminal Support (LVIS) (MicroVention Inc) and Atlas (Stryker) stent-assisted coiling procedures.

METHODS
A total of 459 intracranial aneurysms subjected to coil embolization using LVIS (n = 318) or Atlas stents (n = 141) between April 2015 and December 2019 were eligible for study. To assess occlusive status postembolization, magnetic resonance angiography and/or conventional angiography were used. The Raymond classification was applied to categorize recanalization. Our analysis was propensity score matched according to probability of stent type deployed.

RESULTS
Eventually, 41 aneurysms (8.9%) displayed recanalization (minor, 28; major, 13) 6 mo after coiling. Patient age (P = .018), sex (P = .015), aneurysmal location (P < .001), and type of aneurysm (P < .001) differed significantly by group. Overall and major recanalization rates at midterm were similar in both groups (9.1% and 8.5% vs 3.1% and 2.1%, respectively), and there was no significant difference even after 1:1 propensity score matching (odds ratio [OR] = 0.75 [P = .514] and OR = 0.75 [P = .706], respectively).

CONCLUSION
In stent-assisted coil embolization of intracranial aneurysms, midterm outcomes of LVIS and Atlas device groups were similar, despite theoretic LVIS superiority. Further randomized comparative studies are needed to confirm our findings.
Monetary policy expectations and sovereign risk dynamics in the Eurozone
Authors: Bratis Theodoros , Laopodis Nikiforos T , Kouretas Georgios P 
Published: July 2021

Abstract
We empirically assess the dynamic linkages among sovereign bond yield spreads (both short- and long-term) and monetary policy rates/spreads during the Eurozone’s sovereign and banking crises in the post-2008 period when unconventional monetary policy replaced conventional policy. Our focus is on two subperiods, the crisis period (30 November 2009 to 25 July 2012) and the tranquil period (26 July 2012 to 30 April 2014). For the first subperiod, we noted significant spillovers from the overnight interest spreads spread to almost all sovereign bond yield spreads, at both the mean and volatility levels. Thus, during that period expectations on monetary surprises led bond spreads and their corresponding volatilities compared to the tranquil period, regardless of the bond yields maturities. Overall, we infer that monetary policy and sovereign risk were highly linked during the crisis period and thus, the interest-rate monetary policy transmission mechanism was effective in reducing these yield spreads.
Mechanisms of far-red light-mediated dampening of defense against Botrytis cinerea in tomato leaves
Authors: Courbier Sarah , Snoek Basten L , Kajala Kaisa , Li Linge , van Wees Saskia C M , Pierik Ronald 
Published: July 2021

Abstract
Plants detect neighboring competitors through a decrease in the ratio between red and far-red light (R:FR). This decreased R:FR is perceived by phytochrome photoreceptors and triggers shade avoidance responses such as shoot elongation and upward leaf movement (hyponasty). In addition to promoting elongation growth, low R:FR perception enhances plant susceptibility to pathogens: the growth–defense tradeoff. Although increased susceptibility in low R:FR has been studied for over a decade, the associated timing of molecular events is still unknown. Here, we studied the chronology of FR-induced susceptibility events in tomato (Solanum lycopersicum) plants pre-exposed to either white light (WL) or WL supplemented with FR light (WL+FR) prior to inoculation with the necrotrophic fungus Botrytis cinerea (B.c.). We monitored the leaf transcriptional changes over a 30-h time course upon infection and followed up with functional studies to identify mechanisms. We found that FR-induced susceptibility in tomato is linked to a general dampening of B.c.-responsive gene expression, and a delay in both pathogen recognition and jasmonic acid-mediated defense gene expression. In addition, we found that the supplemental FR-induced ethylene emissions affected plant immune responses under the WL+FR condition. This study improves our understanding of the growth–immunity tradeoff, while simultaneously providing leads to improve tomato resistance against pathogens in dense cropping systems.