bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microsatellite variation in Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) populations from Argentina
Authors: Herrero María I. , Murúa María G. , Casmuz Augusto S. , Gastaminza Gerardo , Sosa‐Gómez Daniel R. 
Published: June 2021

Abstract
Helicoverpa gelotopoeon (Dyar) (Lepidoptera: Noctuidae) is a polyphagous pest that has been reported causing damage in many agricultural crops such as soybean, chickpea and cotton. This species, together with Helicoverpa zea (Boddie), Helicoverpa armigera (Hübner) and Chloridea virescens (Fabricius), belongs to the Heliothinae complex of economic importance in Northwestern Argentina.
Many species in the Heliothinae subfamily have developed resistance to insecticides and insecticidal proteins from Bacillus thuringiensis (Bt). Managing resistance to these control tactics in H. gelotopoeon is of high importance and dependant on knowledge of population genetics of this species.
The objective of this study was to evaluate the genetic variability and structure of H. gelotopoeon populations from different host plants and geographic regions of Argentina. Towards this goal, six microsatellite loci were screened across 196 individuals.
The results obtained revealed high genetic diversity and lack of clear structure among the populations of H. gelotopoeon studied. This genetic homogeneity between populations from different geographical regions and hosts could be attributed to the long‐range migratory capacity of Heliothinae and the generalist behaviour of H. gelotopoeon.
Supporting health students on rural placements: A scoping review
Authors: Rogers Cathy 
Published: June 2021

AbstractObjectiveTo review Australian literature on initiatives used to provide support for pre‐registration health students undertaking a rural placement.DesignA scoping literature review.SettingRural, regional and remote areas of Australia.ParticipantsPublications were sourced from scientific databases including Ebscohost and CINAHL. Grey literature and journal citations were searched to identify other relevant articles.Main outcome measureIdentification of the various initiatives used to support students, evaluation of the success of these initiatives, and the feasibility and sustainability of implementing these initiatives.ResultsThere were 36 articles included in the final analysis. The findings identified support initiatives specific to individual professions, not on supporting health students as a whole cohort. The key findings were grouped into identification of support initiatives and the alignment of these to students feeling a sense satisfaction, belonging and connectedness. Constraints to support health students undertaking rural placements identified disparity and inequity of support initiatives available for health students with medical students provided more support than other health students.ConclusionThis review highlights the importance of students developing a sense of belonging and building connections to community, which are strongly aligned with rural placements and student satisfaction. The need for socio‐cultural, organisational and institutional support is linked to higher student satisfaction and intention to practice rurally. A centralised collation of support initiatives would benefit students, higher education institutions and stakeholders in their efforts to attract students to undertake rural placements and be immersed in these unique learning experiences.
An openly available wearable, a diaper cover, monitors infant's respiration and position during rest and sleep
Authors: Ranta Jukka , Ilén Elina , Palmu Kirsi , Salama Jonna , Roienko Oleksii , Vanhatalo Sampsa 
Published: June 2021

AbstractAimTo describe and test the accuracy of respiratory rate assessment in long‐term surveillance using an open‐source infant wearable, NAPping PAnts (NAPPA).MethodsWe recorded 24 infants aged 1–9 months using our newly developed infant wearable that is a diaper cover with an integrated programmable electronics with accelerometer and gyroscope sensors. The sensor collects child's respiration rate (RR), activity and body posture in 30‐s epochs, to be downloaded afterwards into a mobile phone application. An automated RR quality measure was also implemented using autocorrelation function, and the accuracy of RR estimate was compared with a reference obtained from the simultaneously recorded capnography signal that was part of polysomnography recordings.ResultsAltogether 88 h 27 min of data were recorded, and 4147 epochs (39% of all data) were accepted after quality detection. The median of patient wise mean absolute errors in RR estimates was 1.5 breaths per minute (interquartile range 1.1–2.6 bpm), and the Blandt‐Altman analysis indicated an RR bias of 0.0 bpm with the 95% limits of agreement of −5.7–5.7 bpm.ConclusionLong‐term monitoring of RR and posture can be done with reasonable accuracy in out‐of‐hospital settings using NAPPA, an openly available infant wearable.
Employee–supervisor political skill congruence and work outcomes: The mediating role of leader–member exchange quality
Authors: Öztürk Engin Bağış , Emirza Sevgi 
Published: June 2021

AbstractThis study aims to investigate the effect of employee–supervisor political skill congruence on the quality of leader–member exchange (LMX), which in turn influences employees' job satisfaction and turnover intention. For this purpose, two survey studies were conducted. The first study examined the relationship between employee–supervisor political skill congruence and LMX using multi‐source data collected from 287 employee–supervisor dyads. The second study examined the indirect effect of congruence in political skill on work outcomes through LMX by using time‐lagged multi‐source data collected from 142 employee–supervisor dyads. The findings indicate that compared with incongruence, congruence in political skill is associated with higher employee‐rated LMX. Moreover, among dyads with congruent political skills, congruence at high levels of political skill is associated with higher employee‐rated LMX compared with congruence at low levels of political skill. Moreover, in case of incongruency, low employee–high supervisor political skill combination was related to higher supervisor‐rated LMX compared with high employee–low supervisor political skill combination. Lastly, employee–supervisor political skill congruency indirectly influences job satisfaction and turnover intention through employee‐rated LMX. This research significantly contributes to the political skill literature by examining potential consequences of employee–supervisor congruence in political skill in the workplace.
The clinical impact of robot‐assisted laparoscopic rectal cancer surgery associated with robot‐assisted radical prostatectomy
Authors: Maeda Anri , Takahashi Hiroki , Watanabe Kaori , Yanagita Takeshi , Suzuki Takuya , Nakai Nozomu , Maeda Yuzo , Shiga Kazuyoshi , Hirokawa Takahisa , Ogawa Ryo , Hara Masayasu , Matsuo Yoichi , Takiguchi Shuji 
Published: June 2021

AbstractIntroductionRobot‐assisted laparoscopic surgery has been performed in various fields, especially in the pelvic cavity. However, little is known about the utility of robot‐assisted laparoscopic rectal cancer surgery associated with robot‐assisted radical prostatectomy (RARP). We herein report the clinical impact of robot‐assisted laparoscopic rectal cancer surgery associated with RARP.MethodsWe experienced five cases of robot‐assisted laparoscopic rectal cancer surgery associated with RARP. One involved robot‐assisted laparoscopic abdominoperineal resection with en bloc prostatectomy for T4b rectal cancer, and one involved robot‐assisted laparoscopic intersphincteric resection combined with RARP for synchronous rectal and prostate cancer. The remaining three involved robot‐assisted laparoscopic low anterior resection (RaLAR) after RARP. For robot‐assisted laparoscopic rectal cancer surgery, the da Vinci Xi surgical system was used.ResultsWe could perform planned robotic rectal cancer surgery in all cases. The median operation time was 529 min (373–793 min), and the median blood loss was 307 ml (32–1191 ml). No patients required any transfusion in the intra‐operative or immediate peri‐operative period. The circumferential resection margin was negative in all cases. There were no complications of grade ≥III according to the Clavien‐Dindo classification and no conversions to conventional laparoscopic or open surgery.ConclusionRobot‐assisted laparoscopic surgery associated with RARP is feasible in patients with rectal cancer. The long‐term surgical outcomes remain to be further evaluated.
Enjoying tranquility — Development of ground vegetation after cessation of management in forests on loamy soils in Flanders (Belgium)
Authors: Vandekerkhove Kris , Thomaes Arno , De Keersmaeker Luc , Van de Kerckhove Peter , Onkelinx Thierry , Van Calster Hans , Verheyen Kris 
Published: June 2021

AbstractQuestionsManaged forests often show a more homogeneous age structure compared to unmanaged forests, but also a higher share and frequency of canopy and soil disturbances due to harvest operations. Here, we investigate what happens when non‐intervention is introduced in formerly managed forests:

Was there a significant decrease in vascular plant species richness?
Which species were particularly affected? Are there non‐random shifts in species composition?
Were typical shade‐tolerant forest species also jeopardized due to prolonged deep shade?
LocationFour recently installed strict reserves in Atlantic to Sub‐Atlantic lowland oak and beech forests on fertile loess soils east and southwest of Brussels (Belgium).MethodsWe compared vegetation relevés in permanent plots (183 plots of 16 m × 16 m) with a 10‐year interval. Total species richness per site was derived from rarefaction curves; significance of differences in species richness and composition at plot level were tested using paired t tests and Wilcoxon signed rank tests. Non‐metric multidimensional scaling analysis (NMDS) was used to check for non‐random shifts in species composition.ResultsWe registered significant declines in species richness both at reserve and plot level. This decline was not random, but strongly dependent on ecological traits and strategies, with strong declines in light‐demanding gap phase‐associated species like Lonicera periclymenum and Deschampsia cespitosa, and species requiring soil disturbance, such as Juncus effusus and Carex sylvatica. Shade‐tolerant mesic species like Anemone nemorosa and Allium ursinum clearly increased in frequency and cover, despite strong declines in previous decades.ConclusionThe effect of lower disturbance frequency leading to continued closed canopy, against a background of reduced atmospheric deposition, induces the development of a ground vegetation with lower species richness, due to decrease of species related to disturbance, but with higher dominance and cover of characteristic species of mesic lowland oak and beech forests.
Road verges provide alternative habitats for some, but not all, meadow plants
Authors: Oldén Anna , Pitkämäki Tinja , Halme Panu , Komonen Atte , Raatikainen Kaisa J. 
Published: June 2021

AbstractQuestionsAgricultural intensification has led to the decline of biodiverse meadows and other semi‐natural grasslands. Road verges offer potential alternative habitats for meadow species, but they may not be suitable for all meadow species due to different soil properties, frequent disturbances, pollution or suboptimal management. Are their communities of vascular plants and bryophytes similar or dissimilar to those in mown or grazed meadows? What kind of species are associated with road verges, mown meadows or grazed meadows? How do the habitat types differ in their soil conditions and disturbance intensity?LocationThe study was conducted at 36 sites in central Finland.MethodsWe compared the vascular plant and bryophyte flora and the habitat characteristics of road verges, mown meadows and grazed meadows.ResultsThe community composition of both vascular plants and bryophytes differed among the habitat types. Many species occurred in all three habitat types, but several meadow specialists were absent or less frequent in the road verges. In contrast, road verges hosted more forest species and ruderal species, especially bryophytes. Road verges differed from meadows in their soil conditions.ConclusionsWe conclude that although road verges may host some species typical to meadows, their value as alternative habitats could be increased by improved soil preparation and vegetation management. Meanwhile, the continued decline of quality habitats for meadow species underscores the need to maintain, increase and improve meadow management.
Access to maternity and prenatal care services in rural Michigan
Authors: Wendling Andrea , Taglione Valerie , Rezmer Rachel , Lwin Poe , Frost Jessica , Terhune Julia , Kerver Jean 
Published: June 2021

AbstractBackgroundThe United States physician shortages affect rural health care access, including maternity care. Project aims were to identify and characterize prenatal and delivery care in Michigan's rural counties and to explore access to trial of labor after cesarean (TOLAC) services for women in rural Michigan.MethodsDescriptive, cross‐sectional design used 2015 Medicaid claims data and public health plan information to identify maternity care services provided within Michigan's rural counties. Publicly available health plan information was used to identify rural maternity hospitals and prenatal care practitioners; findings were verified by Internet searches and telephone interviews. Medicaid claims data were used to determine services provided. High‐risk geographic areas were defined as those where women needed to travel >30 miles for maternity‐related care. Expected TOLAC rate was determined based on published national birth data; rural hospitals were stratified based on whether they met the expected TOLAC rate, delivered 20%‐60% of expected rate, or billed ≤1 TOLAC birth to Medicaid in 2015.ResultsIn Michigan's 57 rural counties, only 29 hospitals provide maternity care. Geographic high‐risk areas were identified in the Upper Peninsula and northeast Lower Peninsula of Michigan. Only two rural hospitals billed for the expected rate of TOLAC births; six delivered at a lower rate, and the remaining 21 hospitals provided no TOLAC services, resulting in large areas of the state where women were not offered this option locally.ConclusionsMaternity care services are limited for many rural Michigan women. Findings can be used to target specific strategies to improve access to care for these women. Similar analyses, exploring patterns of maternity care delivery in other rural regions worldwide, may uncover similar or additional inequities.
Impacts of volcanic eruptions and early recovery in freshwater environments and organisms
Authors: Carrillo Uara , Díaz‐Villanueva Verónica 
Published: June 2021

ABSTRACTVolcanic eruptions modify environments physically and chemically with serious consequences for the biota. In this review, we analysed 80 papers reporting the effects of volcanic eruptions in freshwater environments and on freshwater organisms. An increase in water turbidity is the most common reported physical effect while increases in concentrations of inorganic elements, many representing nutrients for primary producers, are the most common chemical effects. Bacterial growth is usually stimulated, while autotrophs can be either positively or negatively affected depending on the type of impact. A persistent effect reported in the biota is changes to the assemblage, which could generate further changes in terms of ecosystem functions. This analysis also identifies some information gaps, particularly involving the effects of eruptions on heterotrophic biofilms in streams and on invertebrates and fish in lakes. Most studies were carried out soon after the volcanic eruption, so it is difficult to assess the recovery of the ecosystems. Eruptions present unique opportunities for scientific discovery, although such studies are often hindered by a lack of pre‐eruption data, which would allow for a more comprehensive assessment of the effects.
Can public spending boost private consumption?
Authors: Asimakopoulos Stylianos , Lorusso Marco , Pieroni Luca 
Published: June 2021

AbstractOne of the most debated issues in modern macroeconomics relates to the behaviour of private consumption in response to an increase in government spending. Recent empirical studies have found a positive relationship between these two macroeconomic fundamentals. However, such a finding cannot be easily reconciled with simple real business cycle models. In this paper, we develop and estimate a new Keynesian model that is able to predict a rise in consumption in response to an increase in productive public spending. We show the two key elements that lead to a statistically significant positive reaction of private consumption, thereby creating consumption present‐value multipliers, are: (i) a productive component in public spending and (ii) nominal rigidities. Our key results remain valid to various robustness checks that include a sub‐sample analysis examining the pre‐Great Recession period and a sensitivity analysis on the structural, fiscal and monetary policy parameters of the model.
Visualization of the cancer cell cycle by tissue‐clearing technology using the Fucci reporter system
Authors: Takahashi Kei , Tanabe Ryo , Ehata Shogo , Kubota Shimpei I. , Morishita Yasuyuki , Ueda Hiroki R. , Miyazono Kohei 
Published: June 2021

AbstractTissue‐clearing technology is an emerging imaging technique currently utilized not only in neuroscience research but also in cancer research. In our previous reports, tissue‐clearing methods were used for the detection of metastatic tumors. Here, we showed that the cell cycles of primary and metastatic tumors were visualized by tissue‐clearing methods using a reporter system. First, we established cancer cell lines stably expressing fluorescent ubiquitination‐based cell cycle indicator (Fucci) reporter with widely used cancer cell lines A549 and 4T1. Fluorescence patterns of the Fucci reporter were investigated in various tumor inoculation models in mice. Interestingly, fluorescence patterns of the Fucci reporter of tumor colonies were different between various organs, and even among colonies in the same organs. The effects of antitumor drugs were also evaluated using these Fucci reporter cells. Of the three antitumor drugs studied, 5‐fluorouracil treatment on 4T1‐Fucci cells resulted in characteristic fluorescent patterns by the induction of G2/M arrest both in vitro and in vivo. Thus, the combination of a tissue‐clearing method with the Fucci reporter is useful for analyzing the mechanisms of cancer metastasis and drug resistance.
MUSASHI‐2 confers resistance to third‐generation EGFR‐tyrosine kinase inhibitor osimertinib in lung adenocarcinoma
Authors: Yiming Reheman , Takeuchi Yasuto , Nishimura Tatsunori , Li Mengjiao , Wang Yuming , Meguro‐Horike Makiko , Kohno Takashi , Horike Shin‐ichi , Nakata Asuka , Gotoh Noriko 
Published: June 2021

AbstractEpidermal growth factor receptor tyrosine kinase inhibitors (EGFR‐TKIs) are effective in patients with non–small‐cell lung cancer (NSCLC) harboring EGFR mutations. However, due to acquired resistance to EGFR‐TKIs, even patients on third‐generation osimertinib have a poor prognosis. Resistance mechanisms are still not fully understood. Here, we demonstrate that the increased expression of MUSASHI‐2 (MSI2), an RNA‐binding protein, is a novel mechanism for resistance to EGFR‐TKIs. We found that after a long‐term exposure to gefitinib, the first‐generation EGFR‐TKI lung cancer cells harboring the EGFR‐TKI‐sensitive mutations became resistant to both gefitinib and osimertinib. Although other mutations in EGFR were not found, expression levels of Nanog, a stemness core protein, and activities of aldehyde dehydrogenase (ALDH) were increased, suggesting that cancer stem‐like properties were increased. Transcriptome analysis revealed that MSI2 was among the stemness‐related genes highly upregulated in EGFR‐TKI‐resistant cells. Knockdown of MSI2 reduced cancer stem‐like properties, including the expression levels of Nanog, a core stemness factor. We demonstrated that knockdown of MSI2 restored sensitivity to osimertinib or gefitinib in EGFR‐TKI‐resistant cells to levels similar to those of parental cells in vitro. An RNA immunoprecipitation (RIP) assay revealed that antibodies against MSI2 were bound to Nanog mRNA, suggesting that MSI2 increases Nanog expression by binding to Nanog mRNA. Moreover, overexpression of MSI2 or Nanog conferred resistance to osimertinib or gefitinib in parental cells. Finally, MSI2 knockdown greatly increased the sensitivity to osimertinib in vivo. Collectively, our findings provide proof of principle that targeting the MSI2‐Nanog axis in combination with EGFR‐TKIs would effectively prevent the emergence of acquired resistance.
Fabrication, workflow and delivery of reconstruction: Summary and consensus statements of group 4. The 6th EAO Consensus Conference 2021
Authors: Jokstad Asbjorn , Pjetursson Bjarni E. , Mühlemann Sven , Wismeijer Daniel , Wolfart Stefan , Fehmer Vincent , Güth Jan Frederik , Holtzman Lucrezia Paterno , Hämmerle Christoph H. F. , Makarov Nikolay , Meijer Henny J.A. , Milinkovic Iva , Sailer Irena , Spitznagel Frank A. , Vandeweghe Stefan , de Velde Tommie Van , Zwahlen Marcel , Giertmuehlen Petra C. 
Published: June 2021

AbstractObjectivesTo report assessments of four systematic reviews (SRs) on (i) clinical outcomes of all‐ceramic implant‐supported crowns (iSCs), (ii) production time, effectiveness, and costs of computer‐assisted manufacturing (CAM), (iii) computer‐assisted implant planning and surgery (CAIPS) time and costs, and (iv) patient‐reported outcome measures (PROMS).Material and MethodsAn author group consisting of experienced clinicians and content experts discussed and evaluated the SRs and formulated consensus on the main findings, statements, clinical recommendations, and need for future research.ResultsAll four SRs were conducted and reported according to PRISMA and detailed comprehensive search strategies in at least three bibliometric databases and hand searching. The search strategies were deemed reproducible. Variation was noted regarding language restrictions and inclusion of grey literature, but the search comprehensiveness appeared persuasive. The SRs included bias risk assessments of the primary studies, and their study methodology impacted the interpretations of the extracted data.Conclusions(i) There is limited evidence (49 NRCT) showing that veneered and monolithic all‐ceramic iSCs have excellent outcomes observed up to 3 years. (ii) There is no evidence evaluating production time and effectiveness comparing subtractive and additive CAM of implant models, abutments and crowns. (iii) There is limited evidence (4 RCT) that CAIPS involves more time and costs when considering the entire workflow and for diagnostics, manufacturing, and insertion of the restoration. Time seems to be the decisive factor for higher costs. (iv) Patients’ comfort increases when optical compared to conventional impressions are used for fabricating iSCs and short‐span FPDs (2 RCT, 5 NRCT).