bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role inBetacoronavirusreplication
Authors: Ogando Natacha S. , Kazzi Priscila El , Zevenhoven-Dobbe Jessika C. , Bontes Brenda W. , Decombe Alice , Posthuma Clara C. , Thiel Volker , Canard Bruno , Ferron François , Decroly Etienne , Snijder Eric J. 
Published: May 2021

AbstractAs coronaviruses (CoVs) replicate in the host cell cytoplasm, they rely on their own capping machinery to ensure the efficient translation of their mRNAs, protect them from degradation by cellular 5’ exoribonucleases, and escape innate immune sensing. The CoV nonstructural protein 14 (nsp14) is a bi-functional replicase subunit harboring an N-terminal 3′-to-5′ exoribonuclease (ExoN) domain and a C-terminal (N7-guanine)-methyltransferase (N7-MTase) domain that is presumably involved in viral mRNA capping. Here, we aimed to integrate structural, biochemical, and virological data to assess the importance of conserved N7-MTase residues for nsp14’s enzymatic activities and virus viability. We revisited the crystal structure of severe acute respiratory syndrome (SARS)-CoV nsp14 to perform anin silicocomparative analysis between betacoronaviruses. We identified several residues likely involved in the formation of the N7-MTase catalytic pocket, which presents a fold distinct from the Rossmann fold observed in most known MTases. Next, for SARS-CoV and Middle East respiratory syndrome-CoV, site-directed mutagenesis of selected residues was used to assess their importance forin vitroenzymatic activity. Most of the engineered mutations abolished N7-MTase activity, while not affecting nsp14-ExoN activity. Upon reverse engineering of these mutations into different betacoronavirus genomes, we identified two substitutions (R310A and F426A in SARS-CoV nsp14) abrogating virus viability and one mutation (H424A) yielding a crippled phenotype across all viruses tested. Our results identify the N7-MTase as a critical enzyme for betacoronavirus replication and define key residues of its catalytic pocket that can be targeted to design inhibitors with a potentialpan-coronaviral activity spectrum.Significance StatementThe ongoing SARS-CoV-2 pandemic emphasizes the urgent need to develop efficient broad-spectrum anti-CoV drugs. The structure-function characterization of conserved CoV replicative enzymes is key to identifying the most suitable drug targets. Using a multidisciplinary comparative approach and different betacoronaviruses, we characterized the key conserved residues of the nsp14 (N7-guanine)-methyltransferase, a poorly defined subunit of the CoV mRNA-synthesizing machinery. Our study highlights the unique structural features of this enzyme and establishes its essential role in betacoronavirus replication, while identifying two residues that are critical for the replication of the four betacoronaviruses tested, including SARS-CoV-2.
The race goes on: A fall armyworm-resistant maize inbred line influences insect oral secretion elicitation activity and nullifies herbivore suppression of plant defense
Authors: ul Malook Saif , Liu Xiao-Feng , Liu Wende , Qi Jinfeng , Zhou Shaoqun 
Published: May 2021

SummaryFall armyworm (Spodoptera frugiperda) is an invasive lepidopteran pest with strong feeding preference towards maize (Zea mays). Its success on maize is facilitated by a suite of specialized detoxification and manipulation mechanisms that curtail host plant defense responses.In this study, we identified a Chinese maize inbred line Xi502 that was able to mount effective defense in response to fall armyworm attack. Comparative transcriptomics analyses, phytohormonal measurements, and targeted benzoxazinoid quantification consistently demonstrate significant inducible defense responses in Xi502, but not in the susceptible reference inbred line B73.In 24 hours, fall armyworm larvae feeding on B73 showed accelerated maturation-oriented transcriptomic responses and more changes in detoxification gene expression compared to their Xi502-fed sibling. Interestingly, oral secretions collected from larvae fed on B73 and Xi502 leaves demonstrated distinct elicitation activity when applied on either host genotypes, suggesting that variation in both insect oral secretion composition and host plant alleles could influence plant defense response.These results revealed host plant adaptation towards counter-defense mechanisms in a specialist insect herbivore, adding yet another layer to the evolutionary arms race between maize and fall armyworm. This could facilitate future investigation into the molecular mechanisms in this globally important crop-pest interaction system.
Force profile of the two-handed hardstyle kettlebell swing in novice older adults: an exploratory profile
Authors: Meigh Neil J. , Hing Wayne A. , Schram Ben , Keogh Justin W.L. 
Published: May 2021

AbstractBackgroundUnderstanding the force profile of an exercise increases clinical confidence when assessing the benefits and potential risks of a prescribed exercise. This exploratory study presents the force profile of the hardstyle kettlebell swing in novice older adults and compares peak force with kettlebell deadlifts. These data will help inform healthcare providers and coaches who are considering prescribing kettlebell exercises for older adults.MethodsThirty-five community-dwelling males and females (59-79 years) were recruited, from applicants to participate in the BELL trial. Two-handed hardstyle swings were performed with 8-16 kg. Deadlifts were performed with 8-24 kg and 8-32 kg for females and males, respectively. Ground reaction force was obtained from a floor-mounted force platform. Pairwise comparisons of peak force, forward force, rate of force development, swing cadence, sex, and kettlebell mass, were investigated for the kettlebell swing, with representative force-time curves described. Pairwise comparisons of peak force, sex and kettlebell mass were investigated for the deadlift, with comparisons of peak force, kettlebell mass, and sex, between swings and deadlifts.ResultsFor kettlebells up to 16 kg, paired samples T-tests show a large exercise effect (δ > 1.4) with peak force higher for swings than deadlifts. Data shows: (i) higher peak force during swings than deadlifts (δ = 1.77), reaching 4.5 (1.0) N.kg-1, (ii) peak force during an 8 kg swing was greater than a 32kg deadlift, (iii) negligible difference in normalised peak force between males and females performing kettlebell swings, but a moderately large effect size during deadlifts (males > females, δ = 0.69), (iv) mean rate of force development of 19.9 (4.7) N.s-1.kg-1 with a very weak, positive correlation with kettlebell mass (y = 14.4 + 0.32x), and trivial effect of sex, (v) mean forward force equal to 5.5% of vertical force during swings, increasing from 3.8 (1.6) % with 8 kg to 7.1 (2.6) % with 16 kg.ConclusionDuring kettlebell swings, there is negligible difference in normalised net peak force between novice males and females using the same absolute loads. Where ground reaction force is a therapeutic target, kettlebell swings with an 8 kg kettlebell could have similar effects to much heavier deadlifts (>24 kg). Kettlebell swings performed with lighter loads, could provider similar therapeutic value to much heavier deadlifts, and may be a more appealing, affordable, and convenient option for older adults.
Study on the corrosion behaviors of high-silicon chromium iron in acidic and alkaline soil solutions
Authors: Lv Kechen , Yang Xinyu , Wu Tangqing , Xu Song , Liu Lanlan , Sun Lin , Wang Xinming 
Published: May 2021

Purpose
High-silicon chromium iron (HSCI) has been used in ground grids in southern China, while there was a lack of study on its corrosion behavior in this soil environment. The purpose of this paper is to discover the corrosion of HSCI in acidic and alkaline soil solutions.

Design/methodology/approach
The original defects on the HSCI surface were observed using optical microscopy, and the corrosion behavior of the HSCI in the acidic and alkaline soil solutions were jointly detected using electrochemical measurements and scanning electron microscopy/energy dispersive spectrometer.

Findings
The results showed the corrosion rates of the HSCI in the acidic and alkaline soil solutions were limited, and the high contents of Cr and Si in matrix was responsible for its high corrosion resistance. The HSCI showed a similar corrosion tendency in the two solutions, while its corrosion rate in the acid soil solution was higher than that in the alkaline soil solution. The corrosion pits on the specimen surface were originated from the original defects in matrix, and the edges of the corrosion pits were more rounded than the original defects after 720 h immersion in the two solutions. The original defects in the HSCI matrix played a significant role in the corrosion process.

Originality/value
The paper discovered the corrosion evolution of HSCI in the acidic and alkaline soil solutions. What is more, the acceleration role of the original defects on the corrosion of the HSCI in the acidic and alkaline soil solutions was discovered in the paper. The results are beneficial for the material selection of ground grid equipment in engineering.
Geographical indication labelling of food and behavioural intentions
Authors: Chen Nai-Hua 
Published: May 2021

PurposeThis paper aims to explore how consumer's purchase intentions and word-of-mouth (WOM) intentions of local tea are influenced by the signal effects of geographical indication labelling (GIL) on the basis of theory of reasoned action and social identity theory.Design/methodology/approachThis study has developed a structural equation model (SEM) to explain the effects of consumer's attitudes, social norms and social identities on behavioural intentions, considering purchase of foods with geographical indication labels. This study collected responses from 318 consumers of tea in Taiwan.FindingsThree main results are reported: (1) the prevention-focused benefit, which is a risk-aversion consideration, and the promotion-focused benefit, which is a taste and joy of quality, have higher influence compared to perceived concerns (price) on attitude towards GIL produce. (2) Compared to subjective norms, consumer attitude affects purchase intention more, yet the effects are opposite when they influence the WOM intentions. (3) Global identity impacts purchase intentions but not WOM intentions, whereas local identity influences both intentions.Originality/valueFindings indicate attitude, social influence and social identity play critical roles in affecting consumer behavioural intentions. Global identity and local identity play different roles in purchase intention and WOM intentions.
Hybrid fuzzy Monte Carlo agent-based modeling of workforce motivation and performance in construction
Authors: Raoufi Mohammad , Fayek Aminah Robinson 
Published: May 2021

Purpose
This paper aims to cover the development of a methodology for hybrid fuzzy Monte Carlo agent-based simulation (FMCABS) and its implementation on a parametric study of construction crew performance.

Design/methodology/approach
The developed methodology uses fuzzy logic, Monte Carlo simulation and agent-based modeling to simulate the behavior of construction crews and predict their performance. Both random and subjective uncertainties are considered in model variables.

Findings
The developed methodology was implemented on a real case involving the parametric study of construction crew performance to assess its applicability and suitability for this context.

Research limitations/implications
This parametric study demonstrates a practical application for the hybrid FMCABS methodology. Though findings from this study are limited to the context of construction crew motivation and performance, the applicability of the developed methodology extends beyond the construction domain.

Practical implications
This paper will help construction practitioners to predict and improve crew performance by taking into account both random and subjective uncertainties.

Social implications
This paper will advance construction modeling by allowing for the assessment of social interactions among crews and their effects on crew performance.

Originality/value
The developed hybrid FMCABS methodology represents an original contribution, as it allows agent-based models to simultaneously process all types of variables (i.e. deterministic, random and subjective) in the same simulation experiment while accounting for interactions among different agents. In addition, the developed methodology is implemented in a novel and extensive parametric study of construction crew performance.
Role of BIM and energy simulation tools in designing zero-net energy homes
Authors: Habibi Shahryar 
Published: May 2021

Purpose
The purpose of this study is to design a zero-energy home, which is known to be capable of balancing its own energy production and consumption close to zero. Development of low-energy homes and zero-net energy houses (ZEHs) is vital to move toward energy efficiency and sustainability in the built environment. To achieve zero or low energy targets in homes, it is essential to use the design process that minimizes the need for active mechanical systems.

Design/methodology/approach
The methodology discussed in this paper consists of an interfacing building information modeling (BIM) tool and a simulation software to determine the potential influence of phase change materials on designing zero-net energy homes.

Findings
BIM plays a key role in advancing methods for architects and designers to communicate through a common software platform, analyze energy performance through all stages of the design and construction process and make decisions for improving energy efficiency in the built environment.

Originality/value
This paper reviews the literature relevant to the role of BIM in helping energy simulation for the performance of residential homes to more advanced levels and in modeling the integrated design process of ZEHs.
Cyber-bullying research in Kenya: a meta-analysis
Authors: Kwanya Tom , Kogos Angella C. , Kibe Lucy Wachera , Ogolla Erick Odhiambo , Onsare Claudia 
Published: May 2021

Purpose
Cyber-bullying is a form of harassment that is perpetrated using electronic media. The practice has become increasingly common especially with the growing ubiquity of social media platforms. Most cyber-bullying cases inevitably occur on Facebook because it is the most preferred social media platform. However, little is known about cyber-bullying research in Kenya. This paper aims to analyse the quantity, quality, visibility and authorship trends of scholarly publications on cyber-bullying from and/or about Kenya.

Design/methodology/approach
This study was conducted as a systematic literature review. A meta-analysis approach was used. Bibliometrics approaches were used to conduct the analysis. Data on the publications was collected from Google Scholar using Harzing's “Publish or Perish” software and then analysed and presented using Microsoft Excel, Notepad and VOSviewer.

Findings
The study yielded 359 research publications on cyber-bullying in Kenya. There was a gradual increment in the number of publications, peaking in 2018. Nearly half of the publications have not been cited indicating low uptake of research on cyber-bullying in Kenya. It also emerged that most of the research has been published on subscription channels thereby restricting their visibility, access and use. Minimal collaboration in research on cyber-bullying in Kenya was also observed since 67.4% of the publications were written by a single (one) author. The authors conclude that the quantity, quality and visibility of research on cyber-bullying in Kenya is low.

Originality/value
This is an empirical study. The findings can be used to promote and mainstream research on cyber-bullying in Kenya.
Against the odds of tradition: nudging the glass ceiling of sport leadership
Authors: Thornton Olivia , Etxebarria Naroa 
Published: May 2021

Purpose
The purpose of this study is to capture the journey of the first female leader in sports management in her country in the south-east Asian region for their National Sport and the values and behaviors enabling her to achieve it.

Design/methodology/approach
The study uses a mixed-methods case study design, quantitative and qualitative outcome measures, to assess the career progression of the first female executive director of a national sporting organization in her country, namely, BWN and her involvement in a two-week intensive sports management, leadership and development program.

Findings
A professional and courageous approach to identify and implement honest and evidence-based solutions were key for BWN’s success. BWN complemented professionalism with soft skills, clear communication and an outcome-based approach to challenging situations, successfully influencing change within her national sporting organization. This female leader is an inspiring role model for other women and her professionalism her most influential value driving innovation in sports management and organizational change. The positive impact she had in her community highlights the significant contributions female leaders can have within the sports leadership context, given the opportunity and scope to do so.

Originality/value
For the first time in her country, the unsolicited and unprecedented evidence-based approach, professionalism and proactivity assisted BWN with her colleagues accepting her as the first woman on the executive board of her national sporting body of one of the most celebrated national sports. Breaking the mold with unexpected (positive) behavior might be the secret to further breakthroughs for women in sports management.
Framework for a resilient religious tourism supply chain for mitigating post-pandemic risk
Authors: Mittal Rajkumari , Sinha Parul 
Published: May 2021

PurposeThis paper aims to study the religious tourism supply chain and understand and introduce resilience across the same to mitigate post-pandemic disruptions.Design/methodology/approachIn this manuscript, a systematic literature review has been done to identify the gaps in the religious tourism supply chain, which gives adequate revenue to India but not studied yet. The identified gap shaped this study's objectives and research questions and guided the authors to devise a theoretical framework for the religious tourism supply chain.FindingsThe key findings of this research paper led to identifying both threats and opportunities for the religious tourism supply chain, which has been into existence and caused many disasters in the past. As pandemic Covid-19 shut the doors of these religious destinations for extended periods, it became necessary for governments, state authorities and private parties to think and devise the post unlock operating processes for this supply chain. This thinking directed the authors to create a framework for the smooth flow of people and other services across this supply chain. The collaborative efforts of all the stakeholders at various levels can realize the actual working of the suggested framework. A stagewise set of processes has been proposed to understand the resilience across the religious tourism supply chain and reduce disruptions.Practical implicationsCovid-19 pandemic has devastated the world economies and disrupted the supply chains of all sectors. The paper elaborates the need for cohesive efforts to introduce resilience across humanitarian supply chains and phase-wise processes to reduce the disruptions caused by various disasters. These systematic efforts will familiarize the readers with the need for resilience across the religious tourism supply chain. Still, it would also assist in generating revenue for the Indian government and reviving the economy soon.Originality/valueThe trade of religious tourism adds a significant contribution to the Indian economy in terms of revenue, employment, visibility of culture and destination, etc. The Covid-19 pandemic has immensely disrupted the tourism sector supply chain, resulting in huge losses (FICCI report 2020). The introduction and implementation of resilience across the religious tourism supply chain can diminish the losses and assist in reviving the economy soon. Construct of resilience across the religious tourism supply chain has not been studied yet. This manuscript contributes to identifying post-pandemic challenges across the religious tourism supply chain and ways to integrate resilience to reduce disruptions caused by disasters.
The combined use of symmetric and asymmetric approaches: partial least squares-structural equation modeling and fuzzy-set qualitative comparative analysis
Authors: Rasoolimanesh S. Mostafa , Ringle Christian M. , Sarstedt Marko , Olya Hossein 
Published: May 2021

Purpose
This study aims to propose guidelines for the joint use of partial least squares structural equation modeling (PLS-SEM) and fuzzy-set qualitative comparative analysis (fsQCA) to combine symmetric and asymmetric perspectives in model evaluation, in the hospitality and tourism field.

Design/methodology/approach
This study discusses PLS-SEM as a symmetric approach and fsQCA as an asymmetric approach to analyze structural and configurational models. It presents guidelines to conduct an fsQCA based on latent construct scores drawn from PLS-SEM, to assess how configurations of exogenous constructs produce a specific outcome in an endogenous construct.

Findings
This research highlights the advantages of combining PLS-SEM and fsQCA to analyze the causal effects of antecedents (i.e., exogenous constructs) on outcomes (i.e., endogenous constructs). The construct scores extracted from the PLS-SEM analysis of a nomological network of constructs provide accurate input for performing fsQCA to identify the sufficient configurations required to predict the outcome(s). Complementing the assessment of the model’s explanatory and predictive power, the fsQCA generates more fine-grained insights into variable relationships, thereby offering the means to reach better managerial conclusions.

Originality/value
The application of PLS-SEM and fsQCA as separate prediction-oriented methods has increased notably in recent years. However, in the absence of clear guidelines, studies applied the methods inconsistently, giving researchers little direction on how to best apply PLS-SEM and fsQCA in tandem. To address this concern, this study provides guidelines for the joint use of PLS-SEM and fsQCA.
Investigating the mechanism linking task conflict with employees’ innovative work behavior
Authors: Al-Ghazali Basheer M. , Afsar Bilal 
Published: May 2021

PurposeThe effect of task conflict on innovative work behavior has yielded inconsistent results pointing to the need to examine the conditions under which task conflict is helpful for employees’ innovative work behavior. This study aims to develop a comprehensive model linking task conflict and innovative work behavior through constructive conflict, positive conflict value, cognitive flexibility and psychological safety.Design/methodology/approachData were collected from 316 supervisor–subordinate dyads working in software development and high-technology companies located in Saudi Arabia. The research model was tested using partial least squares approach.FindingsResults show that constructive conflict mediates the relationship between task conflict and innovative work behavior. Moreover, positive conflict value and cognitive flexibility mediate the effect of constructive conflict on innovative work behavior. Finally, psychological safety positively moderates the effect of positive conflict value and cognitive flexibility on innovative work behavior.Originality/valueThis study suggests that constructive conflict, cognitive flexibility, positive conflict value and psychological safety are important mechanisms that explain the link between task conflict and innovative work behavior.