bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Instability-related MiRNAs Predict Survival, Immune Landscape, and Immunotherapy Responses In Gastric Cancer
Authors: Liu Yaqiong , Cheng Lin , Huang Wei , Cheng Xin , Peng Weijun 
Published: June 2021

Abstract
Backgroud Increasing evidence suggests that microRNAs (miRNAs) are involved in genome instability (GI) and drive the occurrence of tumors. However, the role of GI-related miRNAs in gastric cancer (GC) remains largely unknown. Herein, we developed a novel GI-related miRNA signature (GIMiSig) and further investigated its role in prognosis, the immune landscape, and immunotherapy responses in GC patients. Methods An analysis of somatic mutation data on 434 gastric cancer cases from The Cancer Genome Atlas (TCGA) database was performed, thereby generating genome stability (GS) and GI groups. By detecting differentially expressed miRNAs between the GS and GI groups that were associated with overall survival, 8 miRNAs were identified and used to construct the GIMiSig. Results The GIMiSig showed high accuracy in detecting GC patients. Using GIMiSig to stratify the patients into high- and low-risk subgroups to predict survival outperformed the use of regular clinical features such as age, gender, or disease stage. Patients with low risk had a more favorable survival time than those with high risk. More importantly, the high-risk patients were associated with decreased UBQLN4 expression, higher accumulation of immune cells, lower Titin (TTN) mutation frequency, worse immunotherapy efficacy, and cancer-associated pathways. Conversely, the low-risk patients were characterized by UBQLN4 overexpression, lower fraction of immune cells, higher TTN mutation frequency, better response to immunotherapy, and GI-related pathways. Conclusion In summary, we constructed a novel GIMiSig that could stratify GC patients into distinct risk groups that have different survival outcomes and immunotherapy efficacy. The results may provide new clues for improving GC outcomes.
Guiding Mesenchymal Stem Cells Differentiation into Chondrocytes using Sulfated Alginate / Cold Atmospheric Plasma Modified Polycaprolactone Nanofibrous Scaffold
Authors: Miri Leila , Irani Shiva , Modaress Mohamad Pezeshki , Daemi Hamed , Atyabi Seyed Mohammad 
Published: June 2021

Abstract
Using tissue engineering approaches is one of interesting strategies to repair cartilage injuries. This study reports the preparation of surface-modified electrospun polycaprolactone nanofibrous scaffolds with highly negatively-charged sulfated alginate as functional support for chondrogenic differentiation of mesenchymal stem cells. In this regard, polycaprolactone (PCL) nanofibers were fabricated by electrospinning, surface-activated by cold atmospheric plasma and further surface-modified with aqueous solutions of sulfated alginate. The scanning electron microscopy (SEM) images showed the nanofibrous structure of electrospun PCL mats. The successful surface modification of PCL nanofibrous scaffolds with sulfated alginate was confirmed by the appearance of fingerprint region of mannuronic acid at 812 cm− 1 in attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) spectra. The cytocompatibility of the nanofibrous scaffolds for mesenchymal stem cells (MSCs) was confirmed using MTT assay. Reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry for type 2 collagen marker were conducted to confirm the chondrogenic differentiation of seeded MSCs on the surface of scaffolds. The expression of type 2 collagen by RT-PCR and immunocytochemistry analyses confirmed the chondrogenic differentiation of MSCs. Our results showed that sulfated alginate surface-modified PCL (SM-PCL) nanofibrous scaffold as an appropriate substrate for cell attachment and growth could promote the MSCs differentiate to chondrocytes.
Mobility of the gradient tracking machine in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery
Authors: Wang Xin , Stone David 
Published: June 2021

Abstract
The mating of budding yeast depends on chemotropism, a fundamental cellular process. Haploid yeast cells of opposite mating type signal their positions to one another through the secretion of mating pheromones. We have proposed a deterministic gradient sensing model that explains how these cells orient toward their mating partners. Using the cell-cycle determined default polarity site (DS), cells assemble a gradient tracking machine (GTM) composed of signaling, polarity, and trafficking proteins. After assembly, the GTM redistributes up the gradient, aligns with the pheromone source, and triggers polarized growth toward the partner. Because strong positive feedback mechanisms drive polarized growth at the DS, it is unclear how the GTM is released for tracking after its assembly is complete. What prevents the GTM from triggering polarized growth at the DS? Here we describe two mechanisms that enable tracking. First, the Ras GTPase Bud1 must be inactivated to release the GTM. Second, actin-independent – but not actin-dependent – vesicle delivery must be targeted upgradient to effect GTM redistribution.
Identification of Chronic Stress Biomarkers in Dairy Cows
Authors: Grelet Clément , Dries Virginie Vanden , Leblois Julie , Wavreille José , Mirabito Luc , Soyeurt Hélène , Franceschini Sébastien , Gengler Nicolas , Brostaux Yves , consortium HappyMoo , Dehareng Frédéric 
Published: June 2021

Abstract
Stress in dairy herds can occur from multiple sources. When stress becomes chronic because of a long duration and inability of animals to adapt, it is likely to affect the emotional state, health, immunity, fertility and milk production of cows. While assessing chronic stress in herds would be beneficial, no real consensus has emerged from the literature regarding the indicators of interest. The goal of this study was to compare and evaluate potential biomarkers for chronic stress after inducing stress over a 4-week period through severe overstocking, restricted access to feed and punctual unusual events. A total of 30 cows were involved in the experiment and two similar groups were constituted. Over a 4-week period, the 15 cows of the stress group were housed in overstocked conditions, with 4.6 m² per cow, including resting and feeding areas. In this area, only 7 individual places at the feeding area were available for the 15 cows to generate competition for feed access. Once weekly and during 2 hours, an additional stress was induced by moving cows to an unfamiliar barn and diffusion of stressing noises (dog barking). Meanwhile, the 15 cows of the control group stayed in the original barn, with more than 10 m² per cow and more individual places at the feeding area than cow number. On a weekly basis, several variables considered as potential biomarkers for chronic stress were recorded. Collected data were analyzed using single trait linear repeated mixed models. No differences were observed regarding milk yield, bodyweight of cows or body condition score but the milk loss was more pronounced in the stress group. Whereas no differences were observed in terms of activity between the groups, the standard deviation of activity per cow per week was higher and the rumination of cows was lower in the stress group. The heart rate was lower in the stress group and showed more heterogeneity at the end of the stress period. No differences were observed regarding salivary cortisol, blood glucose, β-endorphin, thyroxine and leucocyte profile. A higher level of hair cortisol and blood fructosamine were observed in the stress group at the end of the stress period. Among the variables highlighted, milk losses and fructosamine were not specific biomarkers for stress while heart rate variables were difficult to collect in practice, consequently the hair cortisol content seemed to be the most promising indicator to assess chronic stress at a large scale.
Biomechanical Study on the Treatment of Acromioclavicular Joint Dislocation by Coracoclavicular Ligament Reconstruction With a Novel EndoButton Plate Combined With Suture Anchor
Authors: Song Zhe , Wang Chen , Yang Na , Zhu Yangjun , Zhang Kun , Qiu Yusheng 
Published: June 2021

Abstract
Purpose This study aimed to assess the biomechanical stability of a novel internal fixation system of EndoButton plate combined with suture anchor in treating acromioclavicular joint dislocation in the cadaveric specimens. In addition, it provides a new method for the clinical treatment of acromioclavicular joint dislocation. Methods Twelve complete shoulder joint specimens were randomly divided into groups A, B, C, and D (n = 3). Firstly, a quasi-static non-destructive circulation experiment was carried out of coracoclavicular ligament until its function failed. Four different internal fixation materials were used to reduce and fix the acromioclavicular joint. Group A was treated with 3.5 mm clavicular hook locking compression plates, Group B with 5 mm suture anchor Group C with 10 mm Endo-button plate, and Group D with a novel combination of 5 mm suture anchor and 10 mm Endo-button plate. Fluoroscopy was performed to undertake the X-ray of the restored acromioclavicular joint, to evaluate the internal fixation position and acromioclavicular joint reduction. Finally, the shoulder joint was fixed firmly on an electronic universal testing machine (100KN) with a self-made stationary fixture, to conduct a destructive static tensile mechanical test of each specimen vertically at a 100 mm/min load speed. The stress-deformation curve was recorded using a computer connected with the universal mechanical testing machine, and the failure strength and reasons for internal fixation were also recorded. Results The average load-to-failure of the coracoclavicular ligament in groups A, B, C, and D was 373.4 ±0.57 N, 373.6 ±0.62 N, 374.4 ±0.68 N, and 373.9 ±0.15 N, respectively (P>0.05). After internal fixation failure, Group A showed two specimens with clavicular fracture, and one with acromial fracture, with an average load-to-failure of 409.8 ±2.92 N. Group B and D showed three specimens with prolapse of anchor, with average load-to-failure of 293.5 ±4.10 N and 374.2 ±0.40 N, respectively. Group C showed three specimens with basilar coracoid fracture, with average load-to-failure of 373.2 ±2.35 N. Statistical differences existed in the biomechanical load of internal fixation failure among the four groups. Group D was statistically different from Group A and Group B, but not Group C. Conclusion The newly designed EndoButton plate combined with suture anchor for coracoclavicular ligament reconstruction was found to boast simple operation and has high feasibility. Thus it was found effective in the reduction of acromioclavicular joint and treatment of acromioclavicular joint dislocation and fitted the biomechanical characteristics of the acromioclavicular joint.
The miR-423-5p/MMP-2 Axis Regulates Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis
Authors: Tzeng Huey-En , Lin Syuan-Ling , Thadevoos Louis Anoop , Ko Chih-Yuan , Liu Ju-Fang , Lin Chih-Yang , Fong Yi-Chin , Tang Chih-Hsin 
Published: June 2021

Abstract

Background: The effects of Nerve growth factor (NGF) in chondrosarcoma are not confirmed, although NGF is capable of promoting the progression and metastasis of several different types of tumors. Here we aim to explore the role of NGF in chondrosarcoma and elucidate how NGF acts.Methods: Immunohistochemistry (IHC)-stained tissue samples from chondrosarcoma patients were stained with NGF and MMP-2 antibodies. Cell migration was examined by Transwell and wound healing assays. The expression levels of MMP-2, microRNA-423-5p (miR-423-5p) were measured by quantitative real-time polymerase chain reaction. MMP-2, FAK, and c-Src protein expression were examined by Western blot assays. The interaction between MMP-2 3’-UTRs and miR-423-5p binding site was explored by luciferase assay. We established the orthotopic in vivo model of chondrosarcoma lung metastasis to further investigate the promoting effects of NGF in metastatic chondrosarcoma.Results: Here, we found that the levels of NGF and matrix metalloproteinase-2 (MMP-2) correlated with tumor stage in patients with chondrosarcoma. NGF facilitated MMP-2-dependent cellular migration in human chondrosarcoma JJ012 cells, while overexpression of NGF enhanced lung metastasis in a mouse model of chondrosarcoma. NGF promoted MMP-2 synthesis and cell migration by inhibiting miR-423-5p expression through the FAK and c-Src signaling cascades. NGF appears to be a worthwhile therapeutic target in the treatment of metastatic chondrosarcoma.Conclusions: Our study has identified that NGF promotes MMP-2-dependent cell migration in human chondrosarcoma tissue by inhibiting miR-423-5p synthesis via the FAK and c-Src signaling cascades
Trifluoro-Icaritin Ameliorates Complete Freund’s Adjuvant-Induced Inflammatory Pain by α7nAChR-Mediated Inhibition of HMGB1/NF-κB Signaling in the Spinal Cord of Rats
Authors: Sun Yalan , Jia Dandan , Xue Meng , Liu Guangsen , Huang Zhihua , Huang Cheng 
Published: June 2021

Abstract
Objectives: To explore whether Trifluoro-icaritin (ICTF) has anti-nociceptive effect on CFA-induced inflammatory pain and its potential mechanisms. Methods: Intraperitoneal injection (0.3, 1.0, and 3.0 mg/kg, i.p.) of ICTF to complete Freund’s adjuvant (CFA)-induced inflammatory pain rats once daily for 21 consecutive days. Pain-related behaviors were evaluated with paw withdrawal threshold (PWT), paw withdrawal latency (PWL), and CatWalk gait analysis. Hematoxylin eosin (HE) staining was applied to determine the morphological alterations in inflamed paw. Molecular docking was conducted to assess the possible targets for ICTF. Expression of pain-related signaling molecules in the spinal cord were detected using qRT-PCR, western blot assay, and immunoﬂuorescence staining. Results:This results showed that ICTF(3.0 mg/kg) effectively alleviated mechanical allodynia and thermal hyperalgesiabut not 0.3 and 1.0 mg/kg in CFA rats. Both paw edema volume and paw tissue inflammatory response were obviously reduced by ICTF. Subsequently, we further observed that ICTF dramatically decreased the mRNA and protein levels of HMGB1, NF-κB p65, and IL-1β but markedly enhanced α7nAChR and IL-10 expression in the spinal cord of CFA rats, and inhibitedthe co-expression of spinal α7nAChR with IBA-1 in double immunofluorescence staining,along with suppressing the alterations of gait parameters induced by CFA. Moreover, Intrathecal injection (i.t.) of α7nAChR antagonist alpha-bungarotoxin (α-Bgtx, 1.0 μg/kg) not only reversed the anti-nociceptive effect of ICTF on pain hypersensitivity, but also inhibited the down-regulation of HMGB1, NF-κB p65, and IL-1β as well as the up-regulation of α7nAChR and IL-10 protein expression induced by ICTF treatment.Conclusion: Our results illustrate that ICTF enables to alleviate CFA-induced inflammatory pain through α7nAChR-mediated inhibition of HMGB1/NF-κB signaling pathway in the spinal cord of rats, suggesting that ICTF may be exploited as a potential painkiller against chronic inflammatory pain.
The Novel LSD1 Inhibitor ZY0511 Suppresses Diffuse Large B Cell Lymphoma Proliferation by Inducing Apoptosis and Autophagy
Authors: Liu Huan , Wei Jing , Sang Na , Zhong Xi , Zhou Xia , Yang Xinyu , Zhang Jing , Zuo Zeping , Zhou Yang , Yang Shengyong , Du Junrong , Zhao Yinglan 
Published: June 2021

Abstract
Lysine-specific demethylase 1 (LSD1, also known as KDM1A) is an attractive agent for treatment of cancer. Nevertheless, the anti-tumor effect of LSD1 inhibitors against diffuse large B-cell lymphoma (DLBCL) and the underlying mechanism are still unclear. Here, we report that KDM1A is overexpressed in human DLBCL tissues, and negatively related to overall survival rate in DLBCL patients. ZY0511, a novel and potent LSD1 inhibitor, developed by our group inhibited the proliferation of human DLBCL cells and blocked cell cycle at G0/G1 phase. Cellular thermal shift assay (CETSA) showed that ZY0511 interacted with LSD1 in DLBCL cells. Furthermore, intraperitoneal administration of ZY0511 significantly suppressed SU-DHL-6 xenograft tumor growth in vivo. Mechanistically, transcriptome sequencing results indicated that ZY0511 induced the genes enrichment significantly related to cell cycle, autophagy and apoptosis signaling pathways. Further study confirmed that ZY0511 decreased mitochondrial membrane potential and induced apoptosis, which can be reverted by a pan-caspase inhibitor, Z-VAD-FMK. Moreover, ZY0511 treatment significantly increased autophagy-associated marker proteins and autophagosomes formation in DLBCL cells. In conclusion, our finding identifies that ZY0511 inhibits DLBCL growth both in vitro and in vivo via the induction of apoptosis and autophagy, and LSD1 inhibitor might be a promising strategy for treating DLBCL.
WITHDRAWN: Study on Adsorption Properties of Loess Calcareous Nodules to Heavy Metalions In Aqueous-solution
Authors: Li Qi , Yanan Li , Shu Xiaoxiao 
Published: June 2021

Abstract
Using calcareous calcareous tuberculosis as adsorbent and heavy metal ions (Cu2+, Zn2+, Cd2+ and Pb2+) as adsorbents, different particle size, adsorption time, pH, adsorbent dosage and initial concentration of heavy metals were studied by single heavy metal adsorption test. And the influence of temperature and other factors on the adsorption of heavy metal ions by calcareous nodules, and determine the optimal conditions for the adsorption of heavy metal ions by calcareous nodules. The results show that the adsorption rates of Cu2+, Zn2+ and Pb2+ decrease gradually with the increase of particle size, but have no obvious effect on Cd2+. With the increase of adsorption time, adsorbent dosage and temperature, the adsorption rates of Cu2+, Zn2+, Cd2+ and Pb2+ gradually increase. The adsorption rate of Cu2+, Zn2+ and Cd2+ decreased gradually with the increase of the initial concentration of heavy metal ions, while the adsorption rate of Pb2+ increased first and then decreased. With the increase of pH, the adsorption rate of Cd2+ increased first and then increased slowly. The adsorption rates of Cu2+, Zn2+ and Pb2+ increased first and then decreased. The adsorption capacity of calcareous nodules to four heavy metal ions is in the order of Pb2+＞Zn2+＞Cu2+＞Cd2+. When the particle size is 0.25 mm, the adsorption time is 120 min, and the dosage is 0.6 g, the calcareous nodules are Pb2+, Zn2+, Cu2+. And Cd2+ can achieve better adsorption, and the adsorption rate can reach 83.33%, 77.78%, 73.81% and 81.93% of its maximum adsorption rate, respectively. The optimal adsorption pH of calcareous nodules to Pb2+, Zn2+, Cu2+ and Cd2+ was 7, 6, 5 and 8, respectively, and the optimal temperature was 50 °C.
Growth and survival performance of the West African mangrove oyster, Crassostrea tulipa cultivated by suspension and bottom culture methods in the Densu Estuary, Ghana
Authors: Osei Isaac Kofi , Yankson Kobina , Obodai Edward Adzesiwor 
Published: June 2021

Abstract
This study compares the efficacy of suspension and bottom culture methods of the West African mangrove oyster, Crassostrea tulipa, on recycled oyster shell cultches in connection with some environmental factors in the Densu Estuary, Ghana, from December 2017 to July 2018. Oyster spat grew up to 5.56 ± 0.10 cm SH for suspension and 4.60 ± 0.14 cm SH for bottom culture on the convex surfaces of oyster shell cultches, whereas oysters cultured on the concave surfaces by suspension and bottom cultured measured 5.59 ± 0.14 cm SH and 4.68 ± 0.14 cm SH, respectively. There was a significant difference (F = 36.26, p = 0.001) between the growth rate of oysters cultured on convex surfaces of cultches by suspension (0.80 ± 0.23 cm/month) and bottom culture (1.02 ± 0.24 cm/month). The growth rate of cultured oysters on concave surfaces of cultches by suspension (1.00 ± 0.24 cm/month) was significantly better (F = 22.32, p < 0.001) than the bottom approach (0.81 ± 0.23 cm/month). Before the extermination of oysters cultured on the bottom in July 2018, there was no significant difference in the survival of oysters cultured by suspension and bottom methods on the convex (χ2 = 0.06, p = 0.99) and concave surfaces (χ2 = 0.19, p = 0.99). Of the physico-chemical factors monitored, oyster growth and survival were significantly influenced by DO (p = 0.004; 0.039) and salinity (p = 0.027; 0.012), respectively. Suspension culture approach should be preferred over the bottom culture, especially for water bodies with low bulk density.
Environmental determinants of the current and future distribution of seven Ottelia Pers. species in African freshwater bodies
Authors: Ngarega Boniface K. , Nzei John M. , Saina Josephat K. , Halmy Marwa Waseem A. , Chen Jin-Ming , Li Zhi-Zhong 
Published: June 2021

Abstract
Understanding the influence of environmental covariates on plants’ distribution is critical, especially for aquatic plant species. Climate change is likely to alter the distribution of aquatic species. However, knowledge of this change on the burden of aquatic macroorganisms is often fraught with difficulty. Ottelia Pers., a model genus for studying the evolution of the aquatic family Hydrocharitaceae, is mainly distributed in slow-flowing creeks, rivers or lakes throughout the world’s pantropical areas. Due to recent rapid climate changes, the natural Ottelia populations have declined significantly. In the present study, we use maximum entropy (MaxEnt) to explore the environmental drivers of seven Ottelia species distribution in African freshwater bodies. The models use known background points to determine how environmental covariates vary spatially and produce continental maps of these species’ distribution. We also estimate the possible influences of the optimistic and extreme pessimistic Intergovernmental Panel on Climate Change (IPCC) pathway scenarios and identify high suitability areas under these scenarios. Model performances were statistically significant than random expectations with Area Under Curve (AUC) values above 0.831, indicating the good performance of the models. We observe that our studied Ottelia species had distinct spatial patterns influenced by climate variations (e.g. Bio9- mean temperature of driest quarter, Bio12- annual precipitation, and Bio14- Precipitation of the driest month). While there is a lack of accord in defining the limiting factors for the distribution of the seven species, it is clear that water-temperature conditions have promising effects when kept within optimal ranges. Niche overlap analyses showed that most Ottelia species have similar but not equivalent climatic niches. In addition, considering the expected warming in the future, the persistence and survival of the Ottelia species in Africa could be compromised. The present findings provide a theoretical basis for the distribution of various Ottelia species in Africa.
Existence and Uniqueness of Solution for Fuzzy Integral Equations of Product Type
Authors: Mahaleh V Samadpor Khalifeh , ezzati reza 
Published: June 2021

Abstract
In this paper, we study the fuzzy Fredholm integral equations of product type. As a special case, integral equations of product type arise, e.g., in the study of the spread of an infectious disease that does not induce permanent immunity. Because of the importance and practicality of such integrals, we prove the existence and uniqueness of the solution of these integral equations with the help of the Banach fixed point theorem. Finally, we obtain an error estimation between the exact solution and the solution of the iterative method. Examples show the applicability of our results.