bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the sensitivity of food webs to multiple stressors
Authors: Beauchesne David , Cazelles Kevin , Archambault Philippe , Dee Laura E. , Gravel Dominique 
Published: July 2021

AbstractEvaluating the effects of multiple stressors on ecosystems is becoming increasingly vital with global changes. The role of species interactions in propagating the effects of stressors, although widely acknowledged, has yet to be formally explored. Here, we conceptualise how stressors propagate through food webs and explore how they affect simulated three‐species motifs and food webs of the Canadian St. Lawrence System. We find that overlooking species interactions invariably underestimate the effects of stressors, and that synergistic and antagonistic effects through food webs are prevalent. We also find that interaction type influences a species’ susceptibility to stressors; species in omnivory and tri‐trophic food chain interactions in particular are sensitive and prone to synergistic and antagonistic effects. Finally, we find that apex predators were negatively affected and mesopredators benefited from the effects of stressors due to their trophic position in the St. Lawrence System, but that species sensitivity is dependent on food web structure. In conceptualising the effects of multiple stressors on food webs, we bring theory closer to practice and show that considering the intricacies of ecological communities is key to assess the net effects of stressors on species.
A new and a described species of Cystiphora (Diptera: Cecidomyiidae) from Japan, with reference to geographically diversified intraspecific populations of C. taraxaci and its host range expansion from native Japanese to alien and hybrid species of Taraxacum (Asteraceae)
Authors: Yukawa Junichi , Kim Wanggyu , Nishino Takako , Minami Tsuneo , Yamauchi Satoshi , Ogawa Makoto , Ohara Kenji 
Published: July 2021

AbstractTwo species of Cystiphora (Diptera: Cecidomyiidae) are newly recorded from Japan. A gall midge that induces leaf galls on Taraxacum species (Asteraceae) is identified as Cystiphora taraxaci (Kieffer). Another gall midge that is responsible for leaf galls on Sonchus brachyotus (Asteraceae) is described as a new species under the name Cystiphora hachijounae Yukawa and Kim based on its incised hypoproct of male terminalia and some of the pupal characteristics. Genetic analysis supported the species identifications and indicated that the Palearctic C. taraxaci has long existed in Japan. It has been diversifying geographically into intraspecific populations since at least 2.57 Ma. Our current study argues against the recent invasion of Japan by alien C. taraxaci at the time of multiple introductions of European Taraxacum species to Japan for use as food (seed oil), forage and greening material during the late 19th century. Genetic analysis of gall‐bearing Taraxacum plants collected from various localities in Japan indicates that most of them were alien or hybrid plants, while native Japanese species were rare, and these plants exhibited polyploidy from 2x to 5x. Cystiphora taraxaci is considered to have expanded its host range from native Japanese to alien and hybrid species of Taraxacum without regard to polyploidy.
Rule‐based preprocessing for data stream mining using complex event processing
Authors: Ramírez Aurora , Moreno Nathalie , Vallecillo Antonio 
Published: July 2021

AbstractData preprocessing is known to be essential to produce accurate data from which mining methods are able to extract valuable knowledge. When data constantly arrives from one or more sources, preprocessing techniques need to be adapted to efficiently handle these data streams. To help domain experts to define and execute preprocessing tasks for data streams, this paper proposes the use of active rule‐based systems and, more specifically, complex event processing (CEP) languages and engines. The main contribution of our approach is the formulation of preprocessing procedures as event detection rules, expressed in an SQL‐like language, that provide domain experts a simple way to manipulate temporal data. This idea is materialized into a publicly available solution that integrates a CEP engine with a library for online data mining. To evaluate our approach, we present three practical scenarios in which CEP rules preprocess data streams with the aim of adding temporal information, transforming features and handling missing values. Experiments show how CEP rules provide an effective language to express preprocessing tasks in a modular and high‐level manner, without significant time and memory overheads. The resulting data streams do not only help improving the predictive accuracy of classification algorithms, but also allow reducing the complexity of the decision models and the time needed for learning in some cases.
A clinical pilot study of salivary gland secretion for xerostomia patients with Sjögren’s syndrome using SPECT/CT
Authors: Minami Yoshiyuki , Ogura Ichiro 
Published: July 2021

AbstractIntroductionThis study examined whether time‐activity curves (TAC) can be used to differentiate between patients with and without Sjögren's syndrome using SPECT/CT.MethodsThirteen xerostomia patients (5 with Sjögren's syndrome and 8 without Sjögren's syndrome) underwent SPECT/CT. Salivary gland secretion data were digitally observed, producing separate time‐activity curves (TAC) for the submandibular glands and parotid glands using SPECT/CT. The salivary gland secretion fraction was defined as A (before stimulation test [counts/frame]) divided by B (after stimulation test [counts/frame]). The A/B ratios of patients with Sjögren's syndrome and those without Sjögren's syndrome were compared using the Mann‐Whitney U test for nonparametric data.ResultsThe A/B ratio of parotid glands with Sjögren's syndrome (1.3 ± 0.2) was significantly lower than for those without Sjögren's syndrome (2.7 ± 0.7). The A/B ratio of submandibular glands with Sjögren's syndrome (1.3 ± 0.4) is significantly lower than for those without Sjögren's syndrome (1.8 ± 0.6).ConclusionThe TAC for the submandibular glands and parotid glands using SPECT/CT may be useful for the objective and quantitative diagnosis of Sjögren's syndrome.
Foreign aid projects and trust in political institutions
Authors: Watkins Mitchell 
Published: July 2021

AbstractDo foreign aid projects undermine citizens' trust in local and national political institutions? Drawing on institutional theories of political trust, I hypothesize that foreign aid projects reduce trust in political institutions by lowering citizens' perceptions of government performance and raising perceptions of corruption. To analyze the impact of aid projects on trust, this article utilizes geolocated survey data on citizens' trust in political institutions from Afrobarometer Rounds 2–5 (2003–2012) and data on the location of foreign aid projects from AidData's Aid Information Management Systems datasets for Nigeria, Senegal, and Uganda. Using a spatial difference‐in‐difference strategy, the empirical results find that active aid projects are on average associated with decreased trust in the president, parliament, and local government council. An exploratory sectoral analysis suggests that the negative average treatment effect is driven by projects in the transportation, agriculture, education, and civil society sectors.
Impact of switching to raltegravir and/or adding losartan in lymphoid tissue fibrosis and inflammation in people living with HIV. A randomized clinical trial
Authors: Torres B , Guardo AC , Squarcia M , Diaz A , Fabra A , Caballero M , Ugarte A , Leal L , Gatell JM , Plana M , Garcia F 
Published: July 2021

BackgroundPersistent inflammation and immune activation are associated with lymph node fibrosis and end‐organ diseases in treatment‐suppressed people living with HIV (PLWH). We investigated the effect of switching to raltegravir and/or adding losartan on lymphoid tissue fibrosis and on the inflammatory/immune‐activation mediators in treated HIV patients.MethodsChronic HIV‐infected patients treated with two nucleoside reverse transcriptase inhibitors (2NRTI) and one non‐NRTI (NNRTI) or protease inhibitor (PI) during at least 48 weeks were randomized to four groups (n = 48): 2NRTI + efavirenz (EFV), 2NRTI + EFV + losartan, 2NRTI + raltegravir and 2NRTI + raltegravir + losartan for 48 weeks. Tonsillar biopsy and peripheral blood markers of CD4 and CD8 T‐lymphocyte activation and senescence, monocyte activation and soluble markers of inflammation were determined at baseline and at week 48 and compared between groups.ResultsNo changes in lymphoid tissue architecture were observed. Adding losartan had no impact on lymphocyte subsets. Conversely, patients who switched to raltegravir showed a higher decrease in all activated [CD4+CD38+HLA‐DR+, −0.3 vs. 0.48 (P = 0.033); CD8+CD38+ HLA‐DR+, −1.6 vs. 1.3 (P = 0.02)] and senescent [CD4+CD28–CD57+, −0.3 vs. 0.26 (P = 0.04); CD8+CD28–CD57+, −6.1 vs. 3.8 (P = 0.002)] T lymphocytes. In addition, the median CD4/CD8 ratio increased by 0.35 in patients in the raltegravir group vs. 0.03 in the other arms (P = 0.002). Differences between groups in monocyte subpopulations or soluble inflammation markers were not observed.ConclusionsLosartan had no effect on lymphoid fibrosis or immune activation/inflammation. Conversely, switching to a regimen with raltegravir significantly decreased activated and senescent T‐lymphocyte subpopulations and increased CD4/CD8 ratio in successfully treated PLWH.
Cytomorphology of normal, reactive, dysmorphic, and dysplastic megakaryocytes in bone marrow aspirates
Authors: Zini Gina , Viscovo Marcello 
Published: July 2021

AbstractThis paper aims to emphasize the importance of applying international consensus guidelines to detect qualitative and quantitative abnormalities of megakaryocytes on smears of bone marrow aspirates (BMA) for a shared and harmonized diagnostic path between different laboratories. Careful evaluation of megakaryocytes on BMA smears represents a cornerstone in the diagnosis of most clonal and nonclonal hematological diseases. Images associated with the detailed morphologic description of normal, reactive, abnormal, and dysplastic megakaryocytes are also reported together with examples of similar cells that, if not promptly identified, can lead to a morphological misdiagnosis.
Laboratory approach to investigation of anemia in pregnancy
Authors: Agarwal Archana M. , Rets Anton 
Published: July 2021

AbstractAnemia is a global health problem in all age groups. According to World Health Organization (WHO), approximately 40% of pregnant women are anemic. Iron deficiency anemia (IDA) due to nutritional deficiency is the most common cause. The incidence of IDA varies worldwide depending on the socioeconomic status, but it remains the leading cause even in developed countries. Physiologic anemia of pregnancy due to relatively higher expansion of blood volume in comparison with elevated red blood cell mass also occurs frequently. Complete blood count (CBC) in the first trimester is recommended for all pregnant women to screen for anemia. The screening of pregnant women for IDA in absence of anemia is still debatable. If IDA is suspected, ferritin level of <30 ng/ml is diagnostic. Iron supplementation is recommended for all pregnant women to compensate the increased demand.
Evaluation of measurable residual disease in multiple myeloma by multiparametric flow cytometry: Current paradigm, guidelines, and future applications
Authors: Soh Kah Teong , Wallace Paul K. 
Published: July 2021

AbstractMultiple myeloma (MM) is a heterogeneous group of mature B‐cell diseases that are typically characterized by the presence and accumulation of abnormal plasma cells (PCs), which results in the excess production of monoclonal immunoglobulin and/or light chain found in the serum and/or urine. Multiparametric flow cytometry (MFC) is an indispensable tool to supplement the diagnosis, classification and monitoring of the disease due to its high patient applicability, excellent sensitivity and encouraging results from various clinical trials. In this regard, minimal or, more appropriately, measurable residual disease (MRD) negativity by MFC has been recognized as a powerful predictor of favourable long‐term outcomes. Before flow cytometry can be effectively implemented in the clinical setting for MM MRD testing, sample preparation, panel configuration, analysis and gating strategies must be optimized to ensure accurate results. This manuscript will discuss the current consensus guidelines for flow cytometric processing of samples and reporting of results for MM MRD testing. We also discuss alternative approaches to detect plasma cells in the presence of daratumumab treatment. Finally, there is a lack of information describing the subclonal distribution of myeloma cells based on their protein expression. The advent of high‐dimensional analysis may assist in following the evolution of antigen expression patterns on abnormal plasma cells in patients with relapsed/refractory disease. This in turn can help identify clonal subtypes that are more aggressive for potential informed decision. An analysis using t‐SNE to identify the emergence of PCs subclones by MFC, along with the analysis of their immunophenotypic profiles are presented as a future perspective.
Gene therapy for hemophilia: Current status and laboratory consequences
Authors: Batty Paul , Lillicrap David 
Published: July 2021

AbstractSince the cloning and characterization of the factor VIII (FVIII) and factor IX genes in the mid‐1980s, gene therapy has been perceived as having significant potential for the treatment of severe hemophilia. Now, some 35 years later, these proposals are close to being realized through the licensing of the first clinical gene therapy product. Adeno‐associated viral vector‐mediated gene therapy for hemophilia A and B has been extensively investigated in preclinical models over the past 20 years, and since 2011, there has been increasing evidence in early phase clinical trials that this therapeutic strategy can provide safe and effective rescue of the hemostatic phenotype in severe hemophilia. As the uptake of hemophilia gene therapy progresses, it is clear that many aspects of the gene therapy process require crucial laboratory support to ensure safe and effective outcomes from his new therapeutic paradigm. These laboratory contributions extend from evaluations of the gene therapy vehicle, assessments of the patient immune status for the vector, and ultimately the performance of assays to determine the hemostatic benefit of the gene therapy and potentially of its long‐term safety on the host genome. As with many aspects of past hemophilia care, the safe and effective delivery of gene therapy will require an informed and coordinated contribution from laboratory science.
Pathogen‐induced inflammation is attenuated by the iminosugar MON‐DNJ via modulation of the unfolded protein response
Authors: Sayce Andrew C. , Martinez Fernando O. , Tyrrell Beatrice E. , Perera Nilanka , Hill Michelle L. , Dwek Raymond A. , Miller Joanna L. , Zitzmann Nicole 
Published: July 2021

AbstractSepsis is a life‐threatening condition involving a dysregulated immune response to infectious agents that cause injury to host tissues and organs. Current treatments are limited to early administration of antibiotics and supportive care. While appealing, the strategy of targeted inhibition of individual molecules in the inflammatory cascade has not proved beneficial. Non‐targeted, systemic immunosuppression with steroids has shown limited efficacy and raises concern for secondary infection. Iminosugars are a class of small molecule glycomimetics with distinct inhibition profiles for glycan processing enzymes based on stereochemistry. Inhibition of host endoplasmic reticulum resident glycoprotein processing enzymes has demonstrated efficacy as a broad‐spectrum antiviral strategy, but limited consideration has been given to the effects on host glycoprotein production and consequent disruption of signalling cascades. This work demonstrates that iminosugars inhibit dengue virus, bacterial lipopolysaccharide and fungal antigen‐stimulated cytokine responses in human macrophages. In spite of decreased inflammatory mediator production, viral replication is suppressed in the presence of iminosugar. Transcriptome analysis reveals the key interaction of pathogen‐induced endoplasmic reticulum stress, the resulting unfolded protein response and inflammation. Our work shows that iminosugars modulate these interactions. Based on these findings, we propose a new therapeutic role for iminosugars as treatment for sepsis‐related inflammatory disorders associated with excess cytokine secretion.
Direct ink writing of vancomycin‐loaded polycaprolactone/ polyethylene oxide/ hydroxyapatite 3D scaffolds
Authors: Zhang Bin , Nguyen Alexander K. , Narayan Roger J. , Huang Jie 
Published: July 2021

AbstractNovel inks were formulated by dissolving polycaprolactone (PCL), a hydrophobic polymer, in organic solvent systems; polyethylene oxide (PEO) was incorporated to extend the range of hydrophilicity of the system. Hydroxyapatite (HAp) with a weight ratio of 55–85% was added to the polymer‐based solution to mimic the material composition of natural bone tissue. The direct ink writing (DIW) technique was applied to extrude the formulated inks to fabricate the predesigned tissue scaffold structures; the influence of HAp concentration was investigated. The results indicate that in comparison to other inks containing HAp (55%, 75%, and 85%w/w), the ink containing 65% w/w HAp had faster ink recovery behavior; the fabricated scaffold had a rougher surface as well as better mechanical properties and wettability. It is noted that the 65% w/w HAp concentration is similar to the inorganic composition of natural bone tissue. The elastic modulus values of PCL/PEO/HAp scaffolds were in the range of 4–12 MPa; the values were dependent on the HAp concentration. Furthermore, vancomycin as a model drug was successfully encapsulated in the PCL/PEO/HAp composite scaffold for drug release applications. This paper presents novel drug‐loaded PCL/PEO/HAp inks for 3D scaffold fabrication using the DIW printing technique for potential bone scaffold applications.
The Community of Inquiry perspective on students' social presence, cognitive presence, and academic performance in online project‐based learning
Authors: Guo Pengyue , Saab Nadira , Wu Lin , Admiraal Wilfried 
Published: July 2021

AbstractProject‐based learning (PjBL) engages students in knowledge acquisition, application, and construction through artefact development. Based on the Community of Inquiry framework, this study characterized college students' social and cognitive presences in online PjBL and examined how presence was related to their academic performance. Twenty‐four groups of students participated in a 3‐week project via WeChat discussion groups and created a final product. Transcripts of students' online discourse were collected and analysed by a coding scheme. The quality of students' artefacts was evaluated by a grading rubric. Descriptive results showed that the component of affectiveness and the level of exploration accounted for the majority of students' social and cognitive presences, respectively. Stepwise regression analyses revealed that certain components and sub‐components of students' social presence, and levels and sub‐levels of their cognitive presence were positively associated with their academic performance. Practical implications for teachers and suggestions for further research are provided.
Comparison of procedural efficacy, balloon nadir temperature, and incidence of phrenic nerve palsy between two cryoballoon technologies for pulmonary vein isolation: A systematic review and meta‐analysis
Authors: Assaf Amira , Bhagwandien Rohit , Szili‐Torok Tamas , Yap Sing‐Chien 
Published: July 2021

AbstractIntroductionIn May 2020, a novel cryoballoon system (POLARx; Boston Scientific) became available for catheter ablation of atrial fibrillation (AF). The design of the cryoballoon is comparable to the Arctic Front Advance Pro (AFA‐Pro; Medtronic), but it is more compliant during freezing. We compared the procedural efficacy, biophysical parameters, and risk of phrenic nerve palsy (PNP) between the two cryoballoons.MethodsEmbase, MEDLINE, Web of Science, Cochrane, and Google Scholar databases were searched until June 1, 2021 for relevant studies comparing POLARx versus AFA‐Pro in patients undergoing pulmonary vein isolation (PVI) for AF.ResultsA total of four studies, involving 310 patients were included. There was no difference between the two groups for outcomes regarding procedural efficacy: acute PVI (odds ratio [OR]: 0.43; 95% confidence interval [CI]: 0.06 to 3.03; p = .40), procedure time (mean difference [MD]: 8.15 min; 95% CI: −8.09 to 24.39; p = .33), fluoroscopy time (MD: 1.32 min; 95% CI: −1.61 to 4.25; p = .38) and ablation time (MD: 1.00 min; 95% CI: −0.20 to 2.20; p = .10). The balloon nadir temperature was lower for all individual pulmonary veins (PV) in POLARx compared with AFA‐Pro (MD: −9.74°C, −9.98°C, −6.72°C, −7.76°C, for left superior PV, left inferior PV, right superior PV, and right inferior PV, respectively; all p < .001). The incidence of PNP was similar between groups (OR: 0.79; 95% CI: 0.22 to 2.85; p = .72).ConclusionIn AF patients undergoing PVI, POLARx and AFA‐Pro had a similar procedural efficacy. Balloon nadir temperatures were lower with POLARx, however, the incidence of PNP was similar.
LncRNA PVT1 promotes the progression of ovarian cancer by activating TGF‐β pathway via miR‐148a‐3p/AGO1 axis
Authors: Wu Yuxian , Gu Wenqian , Han Xiao , Jin Zhijun 
Published: July 2021

AbstractOvarian cancer is a lethal gynaecologic malignancy with poor diagnosis and prognosis. The long non‐coding RNA plasmacytoma variant translocation1 (PVT1) and argonaute 1 (AGO1) are associated with carcinogenesis and chemoresistance; however, the relationship between PVT1 and AGO1 and the downstream mechanisms in ovarian cancer remains poorly known. PVT1 and AGO1 expression was assessed through RT‐qPCR and Western blotting in both human tissues and cell lines. The viability and proliferation of ovarian cancer cells were determined by CCK‐8 assay and TUNEL assay in vitro and immunohistochemistry in vivo. Cell invasion and migration were investigated through transwell and wound‐healing assays. The roles and mechanisms of AGO1 on cell functions were further probed via gain‐ and loss‐of‐function analysis. We reveal that PVT1 expression was significantly increased in ovarian cancer tissues which is associated with advanced FIGO stage, lymph‐node metastasis, poor survival rate, and high expression of AGO1. PVT1 or AGO1 knockdown significantly reduced the cell viability and increased the cell apoptosis and inhibited ovarian tumour growth and proliferation. Furthermore, we discovered that PVT1 up‐regulated the expression of AGO1 and thus regulated the transforming growth factor‐β (TGF‐β) pathway to promote ovarian cancer progression through sponging miR‐148a‐3p. Additionally, the activation of ERK1/2, smad2 and smad4 is observed to be related to the PVT1/miR‐148a‐3p/AGO1/TGF‐β pathway‐induced cascades. Taken together, the present study reveals that PVT1/miR‐148a/AGO1 axis plays an important role in the progression of ovarian cancer and emphasize the potential as a target of value for ovarian cancer therapy.