bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Evidence of Seagrass Environmental Support for Local People’s Economic on the South Coast of Lombok Island
Authors: Syukur A , Idrus A A , Dewi K R , Juniati N , Irmayani  
Published: December 2021

Abstract
The richness of marine biota associated with seagrass has many economic values and has been used by the community as a source of livelihoods such as fish, mollusks, bivalves, crustaceans, and echinoderms. Therefore, this study aims to investigate the contribution of seagrass and its surrounding environment as a source of livelihood for local communities in the study area. This research method uses observation, interviews, questionnaires, and in-depth discussions. Furthermore, the number of samples was determined by purposive sampling, 265 fishers, 128 local non-fishermen, and 125 tourism service providers. The analysis of the average income of respondents (fishermen) based on animal groups obtained from seagrass and surrounding areas is U$16167.5. Meanwhile, the average income of respondents (local non-fishermen) from seagrass areas, especially housewives, is US$3401.63/season (full moon and dead moon) at low tide. Furthermore, the average income of respondents from providers of tourist services (especially for snorkeling, diving, swimming, and transportation services) is U$19353.85/month. The conclusion is that seagrass conservation can be an economical solution for local communities in a global climate change situation.
Limits of acceptable change for sustainable management of the Pelawan Biodiversity Park, Bangka Belitung Islands
Authors: Boentoro M R B , Kisworo  , Wherrett T 
Published: December 2021

Abstract
Indonesia is a country of immense biodiversity, however the natural environment is under pressure because of competing economic needs of the large population. Limits of Acceptable Change is a management strategy which acknowledges that any use of an area brings environmental change and best outcomes are achieved if all stakeholders are involved in the process. LAC was applied to develop a management strategy for Pelawan Biodiversity Park, Bangka Belitung. This reserve existsfor the preservation of endemic flora and fauna and is also utilised by local communities so there exists the potential for conflict of interests. This research was conducted March - April 2021. Through direct observation and interviews with all stakeholders, the LAC process was used to identify the reserve’s environmental and cultural values, and the goals held by each of the stakeholders. The results obtained indicate that the primary values assigned to the park are culture and economy, ecology and conservation, education and research, as well as tourism and ecotourism. Some activities of the communities both within and in the surrounding area have direct negative impacts on the environment in the park, including horticulture, tin mining, palm oil, poaching, over-exploitation of pelawan mushroom and honey, as well as tourist activities. After analysing the potential zoning for all activities, three usage zones were proposed to maximise conservation and facilitate economic sustainability for local communities. Specific management strategies for each zone are proposed together with indicators and standards of environmental change. Practical next steps are proposed for community wide consultation and implementation of management practices.
Evaluation of Apium graveolens from different geographical origins based on TLC-fingerprint and chemometrics
Authors: Kartini K , Jannah M , Wulandari F , Oktaviyanti N D , Setiawan F , Jayani N I E 
Published: December 2021

Abstract

Apium graveolens (celery) has various roles both in the food and medicine sectors. It grows very well in the tropical and subtropical areas of Africa and Asia, including Indonesia. This Apiaceae member contains a number of phytoconstituents, and geographical origin is known to significantly determine the type and concentration of phytochemicals in plant material. This study was carried out to validate and develop thin layer chromatography (TLC)-based fingerprinting combined with chemometrics, i.e., Principal Component Analysis (PCA) and Cluster Analysis (CA), to evaluate the quality of celery harvested from thirteen different geographical origins in Indonesia. The mobile phase was first optimized with a simplex axial design, resulting in 2-propanol, toluene, and dichloromethane (1:6:1) as the optimum mobile phase for a stable and precise TLC system in the celery sample analysis. When analyzed with chemometrics, the TLC-fingerprints could discriminate celeries from various origins. The PCA score plot of the first two principal components (PCs) and CA clearly distinguished the samples’ properties and classified them into four clusters. Samples grouped into one cluster were concluded to have comparable quality, while those in different clusters had different qualities.
Microalgae Isolation found in Kedonganan beach, Badung Bali, Indonesia
Authors: Anggreni A A M D , Arnata I W , Gunam I B W 
Published: December 2021

Abstract
Microalgae are autotrophic organisms that are very abundant in waters. The purpose of this study was to isolate marine microalgae found in Kedonganan Beach, Badung, Bali. In this study, microalgae were isolated by taking phytoplankton samples vertically with a depth of 50 cm at the study site, filtering using a plankton net. The obtained microalgae were observed under a microscope and identified. The results showed that several genera were isolated, namely Nitzschia, Botryococcus, Nannochloropsis, Ceratium, Caetoceros, Skeletonema, Closterium, and Cyclotella. Based on research, Nannochloropsis is a stable genus with a high growth rate. The results of the proximate analysis of biomass showed that the microalgae Nannochloropsis sp. contained 40.30±0.37% carbohydrates, 39.07±0.08% protein, 12.44±0.14% fat, 4.77±0.79% ash and water 3.41±0.37%. Nannochloropsis sp. microalgae isolated from Kedonganan Beach has the potential as a source of protein.
Tannin Concentration of Gyrinops Tea Taken Form Different Agarwood Plantation and Different Processing Method
Authors: Wangiyana I G A S , Supriadi  , Nikmatullah A , Sunarpi  , Mulyaningsih L 
Published: December 2021

Abstract
Tannin is one of the essential components that determine the quality of herbal tea products. This research aims to conduct a quantitative tannin assay of Gyrinops tea with different leaf sampling locations and processing methods. Factorial Completely Randomized Design was used as an experimental design with two treatment factors. The first factor is sampling location (Kekait, Lingsar, and Pejaring). The second factor is the processing method (Fresh leaves and Dried leaves). A titrimetric method with KMnO4 and Indigo carmine reagent was used for quantitative tannin measurement. ANOVA and DMRT at a 5% significant level were used for tannin concentration analysis. The result indicated that the first, second, and interaction factors significantly affected tannin concentration. Drying the G. versteegii leaves could increase the tannin concentration of the Gyrinops tea product. G. versteegii leaves taken from Kekait have shown highest tannin concentration among other regions. It could be concluded that dried G. versteegii leaves Kekait could produce Gyrinops tea with high quality based on tannin concentration measurement.
Effect of medium type, light intensity, and photoperiod on the growth rate of microalgae Chlorococcum sp. local isolate
Authors: Putri D S , Sari D A , Marianah  , Astuti S P , Wangiyana I G A S 
Published: December 2021

Abstract
Microalgae are microscopic organisms that are living in a watery environment, whether in fresh or seawater. As photosynthetic organisms, microalgae are the primary oxygen producers in the water. Furthermore, microalgae have various benefits for the sustainability of human beings. Chlorococcum sp. is green microalgae found in freshwater, seawater, brackish water, or even in wastewater. Publication data on this microalga are limited, but this alga is known for its high lipid content. Previously, Chlorococcum sp. was isolated from the Ampenan Estuary of Lombok Island and grown in a liquid medium using Walne’s. The purpose of this study was to determine the optimum growth factors for the cultivation of Chlorococcum sp. The microalgae growth factors that were varied as treatments were the source of water medium used (distilled water, seawater, and saline water), the light intensity (2000, 25000, 3000, and 3500 lux), and the photoperiod (16: 8; 20:4; and 24:0 hours). Based on the research data, it is known that the type of water media is very influential on the productivity of microalgae. Where the highest growth of Chlorococcum sp. was produced in a medium containing saline water. In addition, the number of cells in the initial culture also affects the life span of microalgae. The treatment with the lowest initial cells caused the cell death phase to be extended, starting from the 11th day of culture. In conclusion, the optimal growth of Chlorococcum sp. occurred on the 5th day with a cell density of 323×104 cells/ml.
Analysis of bioactive compounds present in Kaempferia galanga rhizome collected from different regions of East Java, Indonesia
Authors: Adianingsih O R , Widaryanto E , Saitama A , Zaini A H 
Published: December 2021

Abstract

Kaempferia galanga, also known as kencur in Indonesia, is a medical plant that is traditionally used in various human diseases therapy. Ethyl p-methoxycinnamate (EPMC) is an essential bioactive compound in kencur that plays a role in the management of diseases. Kencur is widely spread in East Java. Thus this study aims to identify and analyze the bioactive compounds in kencur rhizome from a different region in East Java. This study was conducted from June 2020 until November 2020. The samples were collected from 12 regencies in East Java, including Banyuwangi, Blitar, Gresik, Kediri, Lumajang, Madura, Malang, Mojokerto, Nganjuk, Pacitan, Ponorogo, and Trenggalek. A sample from Wonogiri (Central Java) was used as a comparison because its cultivation procedure has been standardized. The altitude of sample accessions location was 15-995 masl (m above sea levels) with the rain intensity of 500-2000 mm3. Based on soil analysis, Banyuwangi had good soil characteristics with high total nitrogen, available P2O5, cation exchange capacity (CEC), and organic carbon. The bioactive compounds were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that all the samples contain three major compounds: EPMC, pentadecane, and (Z)-ethyl cinnamate. The highest content of EPMC was obtained in Wonogiri (79.8%), followed by Malang (78.28%), Blitar (77.23%), and Trenggalek (73.33%). It can be concluded that the kencur samples from Malang, Blitar, and Trenggalek have a high content of EPMC, thus could become the source of kencur used in further medicinal research.
Newborn calf serum supplemented by tellurite as alternative transport medium for Corynebacterium diphtheriae
Authors: Saraswati R D , Nursofiah S , Amalia N , Hartoyo Y , Puspandari N , Sunarno  
Published: December 2021

Abstract
Indonesia is one of the five countries with highest diphtheria cases in the world. Laboratory confirmation by culture method as a gold standard requires bacterial survival. Indonesia’s geographical condition as an archipelagic country and difficulties in transporting clinical samples are often obstacles in maintaining bacterial survival. This study aims to evaluate the ability of several transport mediums to maintain the survival of Corynebacterium diphtheriae. A total of 90 isolates were divided into nine groups of transport mediums. Samples were divided into 2 treatment groups, namely room temperature and temperature 2-8 ºC. On day 2, 4, 8, 16, and 32, 1 isolate from each group with 2 different incubation temperatures was cultured on blood agar medium and incubated for 24 hours at 37 ºC. Bacterial survival was indicated by the growth of suspect colonies which were identified by microscopic and biochemical tests. Results show serum with tellurite can be used with viability lower than silica gel, but higher than other media. Meanwhile at a temperature of 2-8 ºC, there are 2 types of the best transport medium, namely serum with tellurite and open silica gel in aluminum foil. Newborn Calf Serum supplemented with Tellurite can be used as an alternative transport medium for Corynebacterium diphtheriae, both at room temperature and at 2-8 ºC.
Humoral and cellular immunity in mice immunized with whole recombinant yeast expressing complex NS2B/NS3 protein of dengue serotype 3
Authors: Pambudi S , Sulfianti A , Widayanti T , Prihanto A , Juniarti F , Wahyunita K , Gill A , Tarwadi  , Efendi J , Djarot I N , Manalu L P , Lestari C S W 
Published: December 2021

Abstract
A nonpathogenic edible yeast, Saccharomyces cerevisiae, has been identified as a vehicle to express many foreign antigens which elicit the immune response in mice. The complex NS2B/NS3 is a protease that represents a prime target for rational drug design for dengue infection. During infection, the NS3 protein is the main target for CD4+ and CD8+ T cell responses, which may be protective. However, no studies have been undertaken evaluating the use of recombinant yeast Saccharomyces cerevisiae INVSc1 expressing complex NSB/NS3 protease as a protective antigen against dengue infection. In the present study, we evaluated the humoral and cellular immune response elicited by recombinant yeast compared to wild-type yeast in the mouse model. Intraperitoneal (i.p.) administration of recombinant and wild-type yeast at 1 and 25 yeast units into BALB/c mice was used. These studies demonstrated that administration at a low concentration of recombinant yeast at 1 yeast units (YU) significantly elicits antibodies against DENV NS3 antigen. Furthermore, real-time PCR analysis revealed that NS2B/NS3-specific cytocines (TNF-a, IFN-©, IL-2) increased with moderate mode compared to wild-type yeast. The results in this study show the potential of recombinant yeast as an edible vaccine platform against dengue infection.
Efficacy of hepatoprotector jamu formula (combination of Curcuma longa, Curcuma xanthorrhiza, and Taraxacum officinale) compared to Fructus schizandrae extract in mild liver injury: a randomized controlled trial
Authors: Ardiyanto D , Zulkarnain Z , Astana P R W , Triyono A , Novianto F , Fitriani U , Nisa U , Mana T A 
Published: December 2021

Abstract
The prevalence of impaired liver function in developing countries is increasing. Indonesia has several traditional medicines that can be used as alternative treatments for liver dysfunction. The aim of this study was to determine the efficacy of hepatoprotector jamu formula (combination of Curcuma longa, Curcuma xanthorrhiza, and Taraxacum officinale) compared to Fructus schizandrae fruit extract for treating mild liver injury. This study was a RCT using parallel open label design which involved 60 subjects for 42 days of intervention. The parameters used to evaluate efficacy were Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT). There was a significant difference of average SGPT levels on day 21 and day 42 compared to day 0 in both hepatoprotector jamu group and Fructus Schizandrae extract group (p < 0.001). Compared to the baseline, there were a significant difference of average SGOT levels on the follow up days in hepatoprotector jamu group (p=0.023 on day 21; p=0.003 on day 42) as well as Fructus Schizandrae extract group (p=0.028 on day 21; p=0.042 on day 42. The efficacy of hepatoprotector jamu formula was comparable to Fructus schizandrae extract in improving mild liver injury.
Species shifting composition of the Anopheles vector in Wongsorejo district - Banyuwangi, Indonesia
Authors: Senjarini K , Setiawan R , Wathon S , Oktarianti R 
Published: December 2021

Abstract
Malaria’s cases have been reported to occur annually in Wongsorejo district of Banyuwangi, East Java since 2002. However, there is a significant decrease of malaria cases during these last years, which might be related to the malaria vector species shifting composition. The objective of this research was to observe some important bionomic characteristics of malaria vector Anopheles in this area from 2015 until 2020 which include species identification, blood feeding behaviour and biting preference of vector. The data collection i.e. determination of Anopheles diversity and behaviour was conducted monthly for 3-6 months annually from 2015 – 2018. In 2019-2020 we sampled irregularly to see the trend. The results showed that there were species shifting of Anopheles vectors in this area. The proportion of Anopheles (An.) sundaicus and An. subpictus, which were previously reported until 2015 as the main Anopheles species in this area, significantly decreased in 2016 - 2018. An. indefinitus & An. vagus was becoming the majority of Anopheles species. During 2019-2020, An. vagus became the main species identified in the sampling area. The predominant species of An. vagus and An. indefinitus has exophagic and zoophilic preference behaviour. Furthermore, An. indefinitus has not previously been identified as a vector for malaria, compared to An. sundaius, and An. subpictus which are well known as an important primary malaria vector on Java Island, Indonesia.
Extensive anterior myocardial infarction of an older non diabetic patient has better prognosis compared to a younger patient: a case report
Authors: Tanti A , Humaera N N , Rafiq A , Pintaningrum Y 
Published: December 2021

Abstract
ST elevation myocardial infarction (STEMI) incidence rates has been decreased inversely to non-STEMI (121 to 77, 126 to 132 per 100.000 case respectively). Diabetes as a risk factors of STEMI is found in 20% patients. The increment of fibrinogen level in diabetic patient may induce compact clots resistance to fibrinolysis that lead to hypercoagulable state problem. Percutaneous coronary intervention (PCI), a non-surgical invasive procedure, can be done to relieve the obstruction. We reported two case reports, patients with anterior extensive STEMI who had underwent primary PCI. Both patients were found a total occlusion at proximal segment of LAD. First case was presented type 2 diabetes mellitus as comorbid while the second case was without diabetes. High burden thrombus was found on the diabetic patient which had poor prognosis, but on the non diabetic patient was found only less thrombus which was more stable condition. There is a strong correlation between type 2 diabetes mellitus and cardiovascular disease (CVD). Glucose control is important for prevention of CVD. Dietary changes that are characterized by increased use of natural sources diets such as whole grains, vegetables, and fruit-based foods and increased physical activity is remarkably strong factors for diabetes prevention.
Characteristics of Indonesian Society in Utilizing Herbs for Covid Prevention during the Covid-19 Pandemic
Authors: Harfiani Erna , Puspita Ratna , Ramadhani Isniani 
Published: December 2021

Abstract
Until now, Coronavirus disease-19 (Covid-19) is still a significant problem in various countries in the world and Indonesia. Various prevention efforts continue to be carried out by people around the world. In Indonesia, prevention efforts continue to be made, including the use of vaccines and herbs. Herbs are plants that can be used as medicine. This study aims to determine the use of herbal medicines to prevent Covid-19 during the pandemic in several regions in Indonesia. Questionnaires were distributed to the public in several provinces in Indonesia. The questionnaire was given in the form of a G form which was distributed online. The results obtained from the questionnaire show that the Indonesian people who use herbs are more female (66.4%) than male, aged 17-25 years (29.9%), with undergraduate/diploma education (42.7%). At the same time, the types of herbs that are widely consumed are herbal concoctions themselves (45.3%), where the use of these herbal medicines has increased in the last year (41.4%). Herbal medicines can be an alternative for the Indonesian people to prevent being infected with Covid-19.
Assessment of the level of drainage services in Damai settlement area (Ampal River Watershed), Balikpapan, Indonesia
Authors: Nursaiba A , Ulimaz M 
Published: December 2021

Abstract
Flood is a major disaster in Balikpapan City. One of the locations experiencing high flooding is in the Damai settlement area (Ampal River Watershed), especially in Mayor Polisi Zainal Arifin Street. One of the causes of this flood-prone area is the suboptimal drainage conditions. This study aimed to assess the drainage services in the Damai settlement area (Ampal River Watershed), of Mayor Polisi Zainal Arifin Street to observe the drainage services and overcome flood problems. Achieving such goals was done by analyzing the service level of the drainage channel by using the scoring and weighting method. The scoring and weighting method produced classifications of drainage services in the Damai settlement area (Ampal River Watershed) on Mayor Polisi Zainal Arifin Street, namely, medium and bad drainage services. There is segment 2 channel 2A, segment 3 channel 3A, segment 4 channel 4A, and segment 5 on channel 5A and 5B classified as having bad service level and there are 13 channels classified as having moderate service levels.
Potential of urban greening for carbon dioxide reduction from transportation sector
Authors: Meidiana C , Leliana D H , Agustina D 
Published: December 2021

Abstract
Various urban activities which exist in different lands uses in Mojokerto City such as settlement, commercial area, public service, and industrial area form the urban dynamic. Urban dynamic is stimulated by some factors including mobility of people and goods, information, and money. People’s mobility which dominates the urban mobility is accommodated by transportation mode causing transportation sector ranks the top first sector contributing to carbon dioxide (CO2) emissions in urban area, including Mojokerto City. Therefore, the study aims to propose reasonable solution for CO2 emissions reduction from transportation sector. The total amount of CO2 emissions is calculated first, and vegetation capacity to absorb CO2 is calculated afterwards using the formula for emission generation and bio capacity respectively. Development of urban greening is proposed to improve the bio-capacity of urban greening to absorb the remaining emissions. Based on the emission calculation, the total amount of CO2 emissions from transportation sector ranges between 6.6 to to 262.1 ton/year depending on the traffic volume and the distance covered by motorized vehicles. Meanwhile, calculation of bio capacity shows that the average absorption of vegetation in Mojokerto City is only 863.91 ton/yr. Improving urban greening area by planting more trees with higher absorption capacity is proposed to absorb the remaining emission. Calculation shows that enhanced urban greening can increase the emission absorption from 4% to 7% of the total emission from transportation sector.