bluuebunny/crossref_metadata_2025_split huggingface dataset:
C-MYC inducible onco-lncRNA LINC00036 acting as EGFR mRNA stabilizer via RNA-protein and RNA-RNA interactions decreases the sensitivity of gefitinib in human cancer
Authors: Xu Shouping , Wan Lin , Wang Qin , Yin Huizi , Qiao Kun , Li Siwei , Zhang Jinfeng , Wu Hao , Shen Meiying , Ning Shipeng , Pang Da 
Published: February 2021

Abstract
Background: The oncogenic lncRNA based strategies for combating cancer may usher in a new and promising paradigm in cancer therapy. However, few studies have been performed to solve such a critical issue. The complex traits and molecular mechanism of such lncRNAs in tumorigenesis and their relationship with sensitivity of gefitinib in human cancer have not been investigated.Methods: We aimed to identify and validate such a novel oncogenic LINC00036 using transcriptome sequencing approach and a large number of tissue samples of different types of cancer from the our cancer center cohort and public data cohorts from the Cancer Genome Atlas，Gene Expression Omnibus and Cancer Cell Line Encyclopedia. Moreover, series of in vitro and in vivo experiments were performed to examine its roles in tumorigenesis and the sensitivity of gefitinib in different types of cancer cells. Special nanoparticle via a more potent delivery system was developed to investigate the feasibility of targeting LINC00036 in vivo. Furthermore, chromatin immunoprecipitation (ChIP)-sequencing, ChIP, actinomycin D assay, dual-luciferase reporter assay, RNA pull-down and RNA immunoprecipitation were performed were developed to uncover the molecular mechanism.Results: LINC00036 that associated with poor prognosis is significantly upregulated in human cancer tissues. Series of in vitro and in vivo experiments reveal that LINC00036 promotes tumorigenesis and decreases the sensitivity of gefitinib in different types of cancer cells. LINC00036 targeting nanoparticle markedly reduced the growth of human cancer xenografts. Mechanistically, LINC00036 is a direct transcriptional target of c-MYC and a positive feedback loop of the c-MYC-LINC00036-EGFR axis exists in human cancer. LINC00036 acts as an EGFR mRNA stabilizer via RNA-protein and RNA-RNA interactions, inducing the hyper-activation of the downstream AKT and MAPK signaling pathways, which in turn decreases the sensitivity of gefitinib in human cancer.Conclusions: LINC00036, a c-MYC inducible onco-lncRNA, acts an oncogene in human cancer and decreases the sensitivity of gefitinib through positive feedback loop of the c-MYC-LINC00036-EGFR axis. Overall, this study broadens knowledge regarding novel onco-lncRNAs and will assist in developing feasible onco-lncRNAs based-targeted therapeutic strategies to improve the sensitivity of gefitinib in human cancer.
Influence of Preparation Method on the Structural, Linear and Nonlinear Optical Properties of TiN Nanoparticles
Authors: Safa Mahdi , Rajabi Yasser , Ardyanian Mahdi 
Published: February 2021

Abstract
In this paper, titanium nitride nanoparticles were prepared in three methods, In the first method, titanium nitride nanoparticles were prepared by the Ball-mill method; in the second and third methods, the nanoparticles were synthesized by the sol-gel, and the co-precipitation methods. The samples were characterized by X-ray diffraction (XRD), UV-Vis spectroscopy, and scanning electron microscopy (SEM), for the study of structural, linear optical properties, and surface morphology study, respectively. The Z-scan technique was utilized to study the TiN nanoparticles nonlinearity. A change in nonlinear optical behavior with an increase in input pump power and concentration of the TiN nanoparticles was observed. Results showed that the TiN nanoparticles give new potentials in nonlinear optical applications. The nonlinear optical behavior of the TiN nanoparticles shifts from inverse saturation absorption to saturation absorption with increase of intensity.
Tumor-Associated Epilepsy in Patients With Brain Metastases: Necrosis-to-Tumor Ratio Forecasts Postoperative Seizure Freedom
Authors: Bahna Majd , Heimann Muriel , Bode Christian , Borger Valeri , Eichhorn Lars , Güresir Erdem , Hamed Motaz , Herrlinger Ulrich , Ko Yon-Dschun , Lehmann Felix , Potthoff Anna-Laura , Radbruch Alexander , Schaub Christina , Surges Rainer , Weller Johannes , Vatter Hartmut , Schäfer Niklas , Schneider Matthias , Schuss Patrick 
Published: February 2021

Abstract
Surgical resection is highly effective in the treatment of tumor-related epilepsy (TRE) in patients with brain metastases (BM). Nevertheless, some patients suffer from postoperative persistent epilepsy which negatively impacts health-related quality of life. Therefore, early identification of patients with potentially unfavorable seizure outcome after BM resection is important. Patients with TRE that had undergone surgery for BM at the authors’ institution between 2013 and 2018 and were analyzed with regard to preoperatively identifiable risk factors for unfavorable seizure outcome. Tumor tissue and tumor necrosis ratios were assessed volumetrically. According to the classification of the International League Against Epilepsy (ILAE), seizure outcome was categorized as favorable (ILAE 1) and unfavorable (ILAE 2 - 6) after 3 months in order to avoid potential interference with adjuvant cancer treatment.Among all 38 patients undergoing neurosurgical treatment for BM with concomitant TRE, 34 patients achieved a favorable seizure outcome (90%). Unfavorable seizure outcome was significantly associated with larger tumor volumes (p=0.012), a midline shift > 7 mm (p=0.025), and a necrosis/tumor volume ratio > 0.2 (p=0.047).The present study identifies preoperatively collectable risk factors for unfavorable seizure outcome in patients with BM and TRE. This might enable to preselect for highly vulnerable patients with postoperative persistent epilepsy who might benefit from accompanying neuro-oncological expertise during further systemical treatment regimes.
Clinical and Radiographic Outcomes of Cervical Open-Door Laminoplasty Withmodified Centerpiece Mini-Plate Fixation and Extensor Attachment Point Reconstruction in Treating Cervical Spondylotic Myelopathy
Authors: Liu Fa-jing , Liu Yan-cheng , Zhang Ze-pei , Miao Jun 
Published: February 2021

Abstract
ObjectiveThis retrospective study aimed to investigate the clinical and radiographic outcomes of open-door laminoplasty with modified centerpiece mini-plate fixation and extensor attachment point reconstruction for treating cervical spondylotic myelopathy (CSM).MethodSixty-nine patients with CSM, who underwent C3-7 open-door laminoplasty in our hospital from January 2016 to May 2017, were divided into two groups: group A underwent surgery with a modified centerpiece titanium plate and group B underwent surgery with a conventional centerpiece titanium plate. Changes in cervical spinal angle (CSA), cervical range of motion (ROM), atrophy of posterior cervical muscles, and neurological function (Japanese Orthopaedic Association [JOA] score) and the occurrence of axial symptoms (AS) were compared between the groups.ResultThere were no significant differences in operative time, intra-operative blood loss, lamina open angle, and spinal cord drift distance between the groups. After the surgery, JOA score significantly increased (P < 0.05), neurological recovery rates were similar (62.7% vs. 63.4%), cervical ROM did not significantly change when compared with the preoperative level (P > 0.05) in both groups; CSA and cross-sectional area of the posterior cervical muscles decreased significantly in group B (P < 0.05) but not in group A (P > 0.05), and postoperative AS were significantly more severe in group B than in group A (P < 0.05).Conclusion﻿Open-door laminoplasty is an effective surgical procedure for CSM. The application of modified centerpiece mini-plate fixation effectively reconstructs the posterior extensor attachment points, which reduces posterior cervical muscle atrophy, maintains cervical curvature, and reduces the occurrence of axial symptoms.
Femtosecond Laser Micromachining of an Optofluidics-based Monolithic Whispering-Gallery Mode Resonator Coupled to a Suspended Waveguide
Authors: Maia João M. , Amorim Vítor A. , Viveiros Duarte , Marques P. V. S. 
Published: February 2021

Abstract
A monolithic lab-on-a-chip fabricated by femtosecond laser micromachining capable of label-free biosensing is reported. The device is entirely made of fused silica, and consists of a microdisk resonator integrated inside a microfluidic channel. Whispering gallery modes are excited by the evanescent field of a circular suspended waveguide, also incorporated within the channel. Thermal annealing is performed to decrease the surface roughness of the microstructures to a nanometric scale, thereby reducing intrinsic losses and maximizing the Q-factor. Further, it is used to position, with submicrometric precision, the waveguide tangent to the resonator and within the critical coupling regime. With this fabrication method and geometry, the alignment between the waveguide and the resonator is robust and guaranteed at all instances. A maximum sensitivity of 121.5 nm/RIU was obtained at a refractive index of 1.363, whereas near the refractive index range of water-based solutions the sensitivity is 40 nm/RIU. A high Q-factor of 105 is kept throughout the entire measurement range.
Computed Tomography Findings as Determinants of Local and Systemic Inflammation Biomarkers in Interstitial Lung Diseases: A Retrospective Registry-based Descriptive Study
Authors: Lang David , Akbari Kaveh , Horner Andreas , Hepp Magdalena , Kaiser Bernhard , Pieringer Herwig , Lamprecht Bernd 
Published: February 2021

Abstract
Purpose:
To evaluate the association of peripheral blood (PBL) and broncho-alveolar lavage (BAL) biomarkers with inflammatory versus fibrotic high-resolution computed tomography (HRCT) findings in interstitial lung diseases (ILD).
Methods:
HRCT of 127 subsequent ILD-board patients were semi-quantitatively evaluated in a standardized way: Reticulation/honeycombing (RET), traction bronchiectasis (TBR) and emphysema (EMP) were classified as non-inflammatory/fibrotic; consolidations (CON), ground glass opacities (GGO), noduli (NDL) and mosaic attenuation (MOS) as active inflammatory findings. Presence or absence of each HRCT finding was counted in 6 distinct lung regions, resulting scores were graded as minimal (0–1 regions involved), medium (2–4) or extensive (5–6). Associations of routinely assessed PBL/BAL biomarkers with these radiological scores were evaluated using Spearman correlation coefficients and graphical presentation; significance of the graded HRCT scores was tested by applying Kruskal-Wallis tests.
Results:
Blood neutrophil, lymphocyte and eosinophil fraction, neutrophil-lymphocyte ratio (NLR) and BAL lymphocyte fraction consistently showed opposite correlations for inflammatory versus non-inflammatory/fibrotic HRCT finding scores. Blood lymphocyte fraction significantly differed by graded GGO (p = 0.032) and CON (p = 0.027) extent, eosinophil fraction by TBR (p = 0.006) and NLR by CON (p = 0.009). C-reactive protein was significantly related to GGO (p = 0.023) and CON (p = 0.004), BAL lymphocyte fraction to GGO (p = 0.017).
Conclusion:
Blood lymphocyte and eosinophil fraction, NLR, CRP and BAL lymphocyte fraction may aid to differentiate inflammatory from non-inflammatory/fibrotic ILD patterns.
Trial registration:
This evaluation was based on data from the ILD registry of Kepler University Hospital Linz, as approved by the ethics committee of the federal state of Upper-Austria (EK Nr. I-26-17).
Investigating Unique Genes of Five Molecular Subtypes of Breast Cancer Using Penalized Logistic Regression
Authors: raoufi sadegh , Farsangi Saeideh Jafarinejad , Dehesh Tania , Hadizadeh Morteza 
Published: February 2021

Abstract
Background: Breast cancer is the first cancer and fifth cause of death in women around the world. Exploring unique genes for cancers has become interesting. The aim of this study was to explore unique gens of five molecular subtypes of breast cancer in women using penalized logistic regression models.Methods: In this study, microarray data of five independent GEO datasets was combined. This combination includes genetic information of 324 women with breast cancer and 12 healthy women. Lasso logistic regression and adaptive lasso logistic regression were used to extract unique genes. Biological process of extracted gens was evaluated in open-source GOnet web-application. R software version 3.6.0 with glmnet package was used for fitting the models. Results: Totally, 119 genes were extracted among fifteen pairwise comparisons. 17 genes (%14) had overlap between comparative groups. Among 27 genes contributed in positive regulation of cell processes, one gene belonged exclusively to this biological process. Among 46 genes contributed in negative regulation of cell processes, 6 genes belonged exclusively. Among 50 genes that were significant in regulation of metabolism, 4 genes belonged exclusively. Among 32 genes that related to response of stress, 4 genes belonged exclusively. Conclusions: The most genes selected by lasso logistic regression and adaptive Lasso logistic regression, were diagnosed in negative regulation of cell processes.
The Need for Early Management in Patients With&nbsp;COVID-19
Authors: Giraud-Gatineau Audrey , Cortaredona Sébastien , Lagier Jean-Christophe , Million Matthieu , Brouqui Philippe , Obadia Yolande , Peretti-Watel Patrick , Raoult Didier , Gentile Stéphanie 
Published: February 2021

Abstract
In March 2020, the IHU Méditerranée Infection set up a screening and treatment center for patients with COVID-19, a system that has been ultimately recommended by French public health authorities. The recent publication of the profiles of patients hospitalized in France published by the Directorate for Research, Studies, Evaluation and Statistics gives us the opportunity to measure the impact of this multidisciplinary early management system coupled with screening on mortality at 90 days. Analysis of the data shows that the system established at IHU-MI was associated with lower mortality, taking age and sex into account. Regarding the age-standardized mortality rate, mortality rates were lower than national data regardless of the period of the epidemic. Early management seems to have significantly decreased the mortality rate in the under-60 age group, suggesting the importance of early management, regardless of age. In addition, these patients had pejorative clinical criteria (high NEWS-2 score, ICU visits, oxygen saturation below 95%) requiring hospitalization, and co-morbidities that are now known to be aggravating factors [7]. This reinforces the need to care for all individuals, regardless of age. Early medical care, as part of a system integrating a screening center and a day hospital, may explain the lower mortality rates.
Factors Affecting Resolution of Subretinal Fluid After Selective Retina Therapy for Central Serous Chorioretinopathy
Authors: Yamamoto Manabu , Kyo Akika , Hirayama Kumiko , Kohno Takeya , Theisen-Kunde Dirk , Brinkmann Ralf , Miura Yoko , Honda Shigeru 
Published: February 2021

Abstract
The purpose of this study was to investigate the factors of clinical outcome of selective retina therapy (SRT) for central serous chorioretinopathy (CSC). This retrospective study included 77 eyes of 77 patients, who were treated with SRT for CSC and observed at least 6 months after the treatment. SRT laser (527 nm, 1.7 µs, 100 Hz) was used for treatment. The mean best-corrected visual acuity (BCVA) (logMAR), central macular thickness (CMT) and central choroidal thickness were changed from baseline to at 6-months follow-up with significant difference. The multivariate analyses found that the rate of change (reduction) in CMT was associated with focal leakage type on fluorescein angiography (FA) (p = 0.03, coefficient 15.26, 95% confidence interval 1.72 – 28.79) and larger baseline CMT (p < 0.01, coefficient -0.13, 95% confidence interval -0.13 – -0.05). Complete resolution of subretinal fluid was associated with nonsmoking history (p = 0.03, odds ratio 0.276, 95% confidence interval 0.086 – 0.887) and focal leakage type on FA (p < 0.01, odds ratio 0.136, 95% confidence interval 0.042 – 0.437). This result may be useful for predicting the therapeutic effectiveness of SRT.
Enhanced Intestinal Protein Fermentation Correlates With Severe Psychiatric Symptoms of Schizophrenia
Authors: Kang Yu , Liang Ying , Shi Xing , Shen Yang , Huang Zhuran , Wang Jian , Shao Changjun , Chu Yanan , Chen Jing , Yu Jun 
Published: February 2021

Abstract
Emerging findings highlighted the associations of mental illness to nutrition and dysbiosis in the intestinal microbiota, but the underlying mechanisms, especially in schizophrenia (SZ), remain unclarified. Here we conducted a case-control study of SZ by performing gut metagenome, fecal and plasma non-targeted metabolome, short-, medium-, and long-chain fatty acids, and targeted metabolite analysis. The results uncovered an apparent contradiction in SZ patients between inadequate protein intake and protein-fermentation-dominated intestinal microbial metabolism, which shifted from carbohydrate fermentation and protein synthesis in healthy conditions. Moreover, the extent of protein fermentation represented as the abundance of related enzymes and fecal levels of related products, usually nitrogenous and neurologically active, correlated with the severity of psychiatric symptoms. These findings provide a previously uncharacterized pathophysiological process in SZ related to the dysbiosis of gut microbiota and dysregulation in macronutrient metabolism, highlighting the importance of nutrition care and the potentials for developing microbiota-targeted therapeutics in SZ.
Adaptation and validation of the Chinese Version of the Carer Support Needs Assessment Tool for Family Caregivers of Cancer Patients Receiving Home-Based Hospice Care
Authors: Zhou Sijia , Zhao Qianqian , Weng Huimin , Wang Ning , Wu Xia , Li Xinxin , Zhang Lili 
Published: February 2021

Abstract
Objectives: The study objectives were to adapt and validate the Chinese version of the Carer Support Needs Assessment Tool (CSNAT).Methods: Cross-cultural adaptation of the original CSNAT for a Chinese setting was performed according to Brislin’s translation guidelines. A pilot study was conducted with 15 Chinese family caregivers of cancer patients receiving hospice home care. A cross-sectional survey of 205 family caregivers was conducted from December 2018 to May 2019 at a home-based hospice care institute in Shenzhen, China. The validation procedure comprised the establishment of (1) content validity by a group of six experts; (2) face validity by 15 family caregivers; (3) criterion validity by calculating Spearman’s correlations between the CSNAT and caregiving burden, caregiving preparedness and quality of life scales; (4) internal consistency using Cronbach’s alpha.Results: The CSNAT demonstrated good face validity and good content validity (index = 0.98). CSNAT scores showed clear positive correlations with caregiving burden and negative correlations with preparedness for caregiving and quality of life. Internal consistency was high (Cronbach’s alpha = 0.899).Conclusions: The Chinese version of the CSNAT is a valid instrument that is appropriate for identifying needs of family caregivers of cancer patients in home-based hospice care.
Meflin-positive cancer-associated fibroblasts enhance tumour response to immune checkpoint blockade therapy
Authors: Miyai Yuki , Sugiyama Daisuke , Hase Tetsunari , Asai Naoya , Taki Tetsuro , Nishida Kazuki , Fukui Takayuki , Chen-Yoshikawa Toyofumi Fengshi , Kobayashi Hiroki , Mii Shinji , Shiraki Yukihiro , Hasegawa Yoshinori , Nishikawa Hiroyoshi , Ando Yuichi , Takahashi Masahide , Enomoto Atsushi 
Published: February 2021

Abstract
Cancer-associated fibroblasts (CAFs) are an integral component of the tumour microenvironment (TME). Most CAFs shape the TME toward an immunosuppressive milieu and attenuate the efficacy of immune checkpoint blockade (ICB) therapy. However, the detailed mechanism of how heterogeneous CAFs regulate tumour response to ICB therapy has not been defined. Here, we show that a novel CAF subset defined by Meflin, a glycosylphosphatidylinositol-anchored protein marker of mesenchymal stromal/stem cells, is associated with survival and favourable therapeutic response to ICB monotherapy in patients with non-small cell lung cancer (NSCLC). The prevalence of Meflin-positive CAFs positively correlated with CD4-positive T cell infiltration and vascularization within NSCLC tumours. Meflin deficiency and CAF-specific Meflin overexpression resulted in defective and enhanced ICB therapy responses in xenograft tumours in mice, respectively. These findings suggest the presence of a previously unknown CAF subset that promotes ICB therapy efficacy, which adds to our understanding of CAF functions and heterogeneity.
Clinical Outcomes and Safety of Passive Leg Raising in Out-of-Hospital Cardiac Arrest. A Randomized Clinical Trial.
Authors: Azeli Youcef , Bardají Alfredo , Barbería Eneko , Lopez-Madrid Vanesa , Bladé-Creixenti Jordi , Fernández-Sender Laura , Bonet Gil , Rica Elena , Álvarez Susana , Fernández Alberto , Axelsson Christer , Jiménez-Herrera María 
Published: February 2021

Abstract
Background: There are data suggesting that passive leg raising (PLR) improves hemodynamics during cardiopulmonary resuscitation (CPR). This trial aimed to determine the effectiveness and safety of PLR during CPR in out-of-hospital cardiac arrest (OHCA).Methods: We conducted a randomized clinical trial with blinded assessment of the outcomes that assigned adults OHCA to be treated with PLR or in the flat position. The trial was conducted in the Camp de Tarragona region. The main end point was survival to hospital discharge with good neurological outcome defined as Cerebral Performance Category (CPC 1-2). To study possible adverse effects, we assessed the presence of pulmonary complications on the first chest X-rays in survivors and lungs weight from autopsies in non-survivors. Results: In total, 445 randomized CPR attempts were included, 234 were treated with PLR and 211 were controls. Overall, 71.5% were men and the median age was 69 IQR (58-78) years old. At hospital discharge, 4.3% in the PLR group and 4.7% in the control group were alive with CPC 1-2 (OR 0.9; 95% CI 0.4-2.2, p=0.81). A higher survival at hospital admission was found among patients with a shockable rhythm, but there were no significant differences (OR 1.6; 95% CI 0.8-3.4, p=0.18). There were no differences in pulmonary complication rates in chest X-rays (25.9% vs 17.9%, p=0.47) or lung weight 1223 IQR (909.5-1500) mg vs. 1239 IQR (900-1507) mg. Conclusion: In this trial, PLR during CPR did not improve survival to hospital discharge with CPC 1-2. No evidence of adverse effects has been found.Clinical Trial Registration: ClinicalTrials.gov: NCT01952197, registration date: Sept 27, 2013, https://clinicaltrials.gov/ct2/show/NCT01952197.