bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings
Authors: Du Jianghao , Zhu Zhanyun , Yang Junchang , Wang Jia , Jiang Xiaotong 
Published: April 2021

Abstract
In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These agents were used to extract the residual proteins in the non-aged and thermal aged samples. The protein extraction efficiencies of different extracting agents were quantitatively determined by bicinchoninic acid (BCA) method, and then processed by multivariate analysis of variance (MANOVA). The impact of the extraction process on the protein structure was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) and circular dichroism (CD). The results showed that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride (GuHCl) was significantly higher than the other five agents, with less damage to the protein structure during the extraction process.
Evaluation of outcome reporting trends for femoroacetabular impingement syndrome- a systematic review
Authors: Lindman Ida , Nikou Sarantos , Öhlin Axel , Senorski Eric Hamrin , Ayeni Olufemi , Karlsson Jon , Sansone Mikael 
Published: April 2021

Abstract
Purpose
The aim of this systematic review was to evaluate the trends in the literature regarding surgical treatment for femoroacetabular impingement syndrome (FAIS) and to present which patient-reported outcome-measures (PROMs) and surgical approaches are included.

Methods
This systematic review was conducted with the PRISMA guidelines. The literature search was performed on PubMed and Embase, covering studies from 1999 to 2020. Inclusion criteria were clinical studies with surgical treatment for FAIS, the use of PROMs as evaluation tool and studies in English. Exclusion criteria were studies with patients < 18 years, cohorts with < 8 patients, studies with primarily purpose to evaluate other diagnoses than FAIS and studies with radiographs as only outcomes without using PROMs. Data extracted were author, year, surgical intervention, type of study, level of evidence, demographics of included patients, and PROMs.

Results
The initial search yielded 2,559 studies, of which 196 were included. There was an increase of 2,043% in the number of studies from the first to the last five years (2004–2008)—(2016–2020). There were 135 (69%) retrospective, 55 (28%) prospective and 6 (3%) Randomized Controlled Trials. Level of evidence ranged from I-IV where Level III was most common (44%). More than half of the studies (58%) originated from USA. Arthroscopic surgery was the most common surgical treatment (85%). Mean follow-up was 27.0 months (± 17 SD), (range 1.5–120 months). Between 1–10 PROMs were included, and the modified Harris Hip Score (mHHS) was most commonly used (61%).

Conclusion
There has been a continuous increase in the number of published studies regarding FAIS with the majority evaluating arthroscopic surgery. The mHHS remains being the most commonly used PROM.
Human biodistribution and dosimetry of [11C]-UCB-J, a PET radiotracer for imaging synaptic density
Authors: Cawthorne Christopher , Maguire Paul , Mercier Joel , Sciberras David , Serdons Kim , Bormans Guy , de Hoon Jan , Van Laere Koen , Koole Michel 
Published: April 2021

Abstract
Rationale
[11C]-UCB-J is an emerging tool for the noninvasive measurement of synaptic vesicle density in vivo. Here, we report human biodistribution and dosimetry estimates derived from sequential whole-body PET using two versions of the OLINDA dosimetry program.

Methods
Sequential whole-body PET scans were performed in 3 healthy subjects for 2 h after injection of 254 ± 77 MBq [11C]-UCB-J. Volumes of interest were drawn over relevant source organs to generate time-activity curves and calculate time-integrated activity coefficients, with effective dose coefficients calculated using OLINDA 2.1 and compared to values derived from OLINDA 1.1 and those recently reported in the literature.

Results
[11C]-UCB-J administration was safe and showed mixed renal and hepatobiliary clearance, with largest organ absorbed dose coefficients for the urinary bladder wall and small intestine (21.7 and 23.5 μGy/MBq, respectively). The average (±SD) effective dose coefficient was 5.4 ± 0.7 and 5.1 ± 0.8 μSv/MBq for OLINDA versions 1.1 and 2.1 respectively. Doses were lower than previously reported in the literature using either software version.

Conclusions
A single IV administration of 370 MBq [11C]-UCB-J corresponds to an effective dose of less than 2.0 mSv, enabling multiple PET examinations to be carried out in the same subject.

Trial registration
EudraCT number: 2016-001190-32. Registered 16 March 2016, no URL available for phase 1 trials.
Poverty and family resilience in Belém-Pará
Authors: Matos Larissa Araújo , Leal Edson Marcos Ramos , Pontes Fernando Augusto Ramos , Costa e Silva Simone Souza 
Published: April 2021

AbstractFamily resilience is a complex, multi-determined behavior caused by the inseparable action of risk and protection factors. The purpose of this paper is to associate aspects of family resilience with multiple dimensions of poverty through a quantitative, descriptive, correlative, exploratory study with a sample of 448 low-income families in thirteen Social Assistance Reference Centers in Belém, Pará. The instruments used in the study were the Family Resilience Profile Questionnaire, the Social and Demographic Inventory, and the Family Poverty Rate. The results state that the families are not living in extreme poverty; however, they still face adversities due to the poverty. A significant presence of women, where 90.6% of the participants were mothers living in a single-parent family, attests that women are still the part of the population most affected by poverty. Furthermore, the results showed that the higher the poverty level, the lower the family resilience, and aspects such as work, knowledge and human development, especially child development, are aspects that enhance family resources to face adversities.
Metatranscriptomic analyses reveal ruminal pH regulates fiber degradation and fermentation by shifting the microbial community and gene expression of carbohydrate-active enzymes
Authors: Li Meng M. , White Robin R. , Guan Le Luo , Harthan Laura , Hanigan Mark D. 
Published: April 2021

AbstractBackgroundVolatile fatty acids (VFA) generated from ruminal fermentation by microorganisms provide up to 75% of total metabolizable energy in ruminants. Ruminal pH is an important factor affecting the profile and production of VFA by shifting the microbial community. However, how ruminal pH affects the microbial community and its relationship with expression of genes encoding carbohydrate-active enzyme (CAZyme) for fiber degradation and fermentation are not well investigated. To fill in this knowledge gap, six cannulated Holstein heifers were subjected to a continuous 10-day intraruminal infusion of distilled water or a dilute blend of hydrochloric and phosphoric acids to achieve a pH reduction of 0.5 units in a cross-over design. RNA-seq based transcriptome profiling was performed using total RNA extracted from ruminal liquid and solid fractions collected on day 9 of each period, respectively.ResultsMetatranscriptomic analyses identified 19 bacterial phyla with 156 genera, 3 archaeal genera, 11 protozoal genera, and 97 CAZyme transcripts in sampled ruminal contents. Within these, 4 bacteria phyla (Proteobacteria,Firmicutes,Bacteroidetes, andSpirochaetes), 2 archaeal genera (Candidatus methanomethylophilusandMethanobrevibacter), and 5 protozoal genera (Entodinium,Polyplastron,Isotricha,Eudiplodinium, andEremoplastron) were considered as the core active microbes, and genes encoding for cellulase, endo-1,4-beta- xylanase, amylase, and alpha-N-arabinofuranosidase were the most abundant CAZyme transcripts distributed in the rumen. Rumen microbiota is not equally distributed throughout the liquid and solid phases of rumen contents, and ruminal pH significantly affect microbial ecosystem, especially for the liquid fraction. In total, 21 bacterial genera, 4 protozoal genera, and 6 genes encoding CAZyme were regulated by ruminal pH. Metabolic pathways participated in glycolysis, pyruvate fermentation to acetate, lactate, and propanoate were downregulated by low pH in the liquid fraction.ConclusionsThe ruminal microbiome changed the expression of transcripts for biochemical pathways of fiber degradation and VFA production in response to reduced pH, and at least a portion of the shifts in transcripts was associated with altered microbial community structure.
Nonequilibrium-DMFT based RIXS investigation of the two-orbital Hubbard model
Authors: Werner Philipp , Johnston Steven , Eckstein Martin 
Published: April 2021

Abstract
Resonant inelastic X-ray scattering (RIXS) detects various types of high- and low-energy elementary excitations in correlated solids, and this tool will play an increasingly important role in investigations of time-dependent phenomena in photo-excited systems. While theoretical frameworks for the computation of equilibrium RIXS spectra are well established, the development of appropriate methods for nonequilibrium simulations is an active research field. Here, we apply a recently developed nonequilibrium dynamical mean field theory (DMFT) based approach to compute the RIXS response of photo-excited two-orbital Mott insulators. The results demonstrate the feasibility of multi-orbital nonequilibrium RIXS calculations and the sensitivity of the quasi-elastic fluorescence-like features and

excitation peaks on the nonequilibrium population of the Hubbard bands.
Recognition of position-specific properties of tectal cell membranes by retinal axons in vitro
Authors: Walter Jochen , Kern-Veits Brigitte , Huf Julita , Stolze Bernd , Bonhoeffer Friedrich 
Published: April 2021

ABSTRACT
In order to test the preference of growing axons for membrane-associated positional specificity a new in vitro assay was developed. In this assay, membrane fragments of two different sources are arranged as a carpet of very narrow alternating stripes. Axons growing on such striped carpets are simultaneously confronted with the two substrates at the stripe borders. If there is a preference of axons for one or the other substrate they become oriented by the stripes and grow within the lanes of the preferred substrate. Such preferential growth could, in principle, be due to affinity to attractive factors on the preferred stripes or avoidance of repulsive factors on the alternate stripes. This assay system was used to investigate growth of chick retinal axons on tectal membranes. Tissue strips cut from various areas of the retina were explanted and the extending axons were confronted with stripes of cell membranes from various areas within the optic tectum.
Tectal cell membranes prove to be an excellent substrate for the growth of retinal axons. Nasal and temporal axons can grow well on membranes of both posterior and anterior tectal cells. If, however, temporal axons are given a choice and encounter the border between anterior and posterior membranes they show a marked preference for growth on membranes of the anterior tectum, their natural target area. Nasal axons do not show a preference in this assay system. The transition from nasal to temporal properties within the retina is abrupt. In contrast, the transition from anterior to posterior properties of the tectal cell membranes occurs as a smooth gradient.
Significantly, the positional differences of tectal membrane properties are only seen during the period of development of the retinotectal projection and are independent of tectai innervation by retinal axons. These anterior-posterior differences disappear by embryonic day 14.
The effects of tectal lesion on the survival of isthmic neurones in Xenopus
Authors: Straznicky Charles , Mccart Roger 
Published: April 2021

ABSTRACT
The isthmic nucleus (IN) is a visual relay centre of the frog brain. It receives afferent projection from the optic tectum of the same side and projects bilaterally to both tecta. In young postmetamorphic Xenopus frogs, the survival of neurones in the IN on both sides was studied following the complete removal of the right tectum.
In 6- to 8-week-old frogs, the right tectum was surgically removed and the operated animals allowed to survive for 1 to 13 weeks after operation. In selected animals, 3 days before the intended sacrifice, the postoptic commissure was transected and the cut isthmotectal fibres filled with horseradish peroxidase (HRP). In serial paraffin sections of the midbrain, the numbers of surviving and dying (pyknotic) neurones in the left and right IN were counted. The soma size of viable isthmic neurones and the volume of both IN were measured.
Pyknotic neurones were seen between 1 and 6 weeks after operation in both the left and right IN, although the rate of cell loss was much greater in the latter.
Virtually all the neurones of the right IN degenerated by 6 weeks after tectal ablation. In contrast, approximately 60 % of neurones of the left IN survived. HRP histochemistry showed labelled isthmic neurones both in the left and right IN up to 3 weeks after operation. Thereafter, HRP-labelled neurones appeared only in the left IN.
These observations indicate that the removal of the natural target of isthmic neurones brings about severe neurone death. If target removal is accompanied by deafferentation, as is the case in the right IN, isthmic neurone death is exacerbated and all neurones die within a short period of 6 weeks. The 60 % neurone survival in the left IN suggests that a very substantial proportion of isthmic neurones projects initially, or is channelled as a result of tectal lesion, to the ipsilateral tectum.
Ordering of Y-specific sequences by deletion mapping and analysis of X-Y interchange males and females
Authors: Ferguson-Smith M. A. , Affara N. A. , Magenis R. E. 
Published: April 2021

ABSTRACT
We have used DNA from 23 patients with Y-chromosome aberrations and 25 patients with presumptive X-Y interchange to map 39 Yp restriction fragments and 37 Yq restriction fragments. In the majority of patients the results are consistent with a standard contiguous order of sequences along the Y chromosome. In 6 of 26 patients (23 %) with Yp aberrations and 2 of 17 (12 %) with Yq aberrations, exceptions to the consensus order have been observed. These can be accommodated by postulating the presence of inversion polymorphisms. Such variation may occur more commonly on the nonpairing part of the Y chromosome that in other chromosomes owing to the absence of homologous synapsis and recombination in male meiosis.
The Y sequence most frequently present in X-Y interchange males was that recognized by GMGY3. 18 of 19 X-Y interchange males had this sequence suggesting that it is the nearest in the series to the TDF locus, and indicating that the latter maps to the distal end of Yp. Several techniques, including in situ hybridization and DNA measurement by flow cytometry, have been used to demonstrate that in X-Y interchange males there is transfer of Y sequences to the distal end of the X chromosome; no mechanism other than X-Y interchange has been demonstrated.
Primary sex determination in the nematode C. elegans
Authors: Hodgkin Jonathan 
Published: April 2021

ABSTRACT
Most nematodes have XO male/XX female sex determination. C. elegans is anomalous, having XX hermaphrodites rather than females. The hermaphrodite condition appears to result from the modification of a basic male/female sex-determination system, which permits both spermatogenesis and oogenesis to occur within a female soma. This modification is achieved by a germ-line-specific control acting at one step in a cascade of autosomal regulatory genes, which respond to X-chromosome dosage and direct male, female, or hermaphrodite development. Mutations of one of these genes can be used to construct artificial strains with ZZ male/WZ female sex determination.
Primary sex determination normally depends on the ratio of X chromosomes to autosomes, as in Drosophila, and there appear to be multiple sites on the X chromosome that contribute to this ratio. Also, as in Drosophila, X-chromosome expression is compensated to equalize gene activity in XX and XO animals. Interactions between dosage compensation and sex determination are described and discussed.
Polytene chromosomes in mouse trophoblast giant cells
Authors: Varmuza Susannah , Prideaux Valerie , Kothary Rashmi , Rossant Janet 
Published: April 2021

Abstract
Mouse trophoblast giant cells undergo successive rounds of DNA replication resulting in amplification of the genome. It has been difficult to determine whether giant cell chromosomes are polyploid as in liver cells or polytene as in Dipteran salivary glands because the chromosomes do not condense. We have examined the pattern of hybridization of mouse giant cells with a variety of in situ chromosome markers to address this question. Hemizygous markers displayed one hybridization signal per nucleus in both diploid and giant cells, while homozygous markers displayed two signals per nucleus in both cell types. These patterns are consistent with cytological evidence indicating that giant cell chromosomes are polytene rather than polyploid. However, in contrast to the situation in Dipteran salivary glands, the two homologues do not appear to be closely associated. We conclude that the mechanism of giant cell DNA amplification involves multiple rounds of DNA replication in the absence of both karyokinesis and cytokinesis, and that sister chromatids, but not homologous chromosomes, remain closely associated during this process.
Graded requirement for the zygotic terminal gene,tailless, in the brain and tail region of theDrosophilaembryo
Authors: Strecker Teresa R. , Merriam John R. , Lengyel Judith A. 
Published: April 2021

AbstractWe have used hypomorphic and null tailless (tll) alleles to carry out a detailed analysis of the effects of the lack of tll gene activity on anterior and posterior regions of the embryo. The arrangement of tll alleles into a continuous series clarifies the relationship between the anterior and posterior functions of the tll gene and indicates that there is a graded sensitivity of anterior and posterior structures to a decrease in tll gene activity. With the deletion of both anterior and posterior pattern domains in tll null embryos, there is a poleward expansion of the remaining pattern. Using anti-horseradish peroxidase staining, we show that the formation of the embryonic brain requires tll. A phenotypic and genetic study of other pattern mutants places the tll gene within the hierarchy of maternal and zygotic genes required for the formation of the normal body pattern. Analysis of mutants doubly deficient in tll and maternal terminal genes is consistent with the idea that these genes act together in a common pathway to establish the domains at opposite ends of the embryo. We propose that tll establishes anterior and posterior subdomains (acron and tail regions, respectively) within the larger pattern regions affected by the maternal terminal genes.
Mouse homeobox gene transcripts occupy different but overlapping domains in embryonic germ layers and organs: a comparison of Hox-3.1 and Hox-1.5
Authors: Gaunt Stephen J. 
Published: April 2021

Abstract
By use of in situ hybridization experiments, the mouse homeobox genes Hox-3.1 and Hox-1.5 are compared in the temporal and spatial patterns of their embryonic transcripts. Transcripts of both genes are first detected at about days, although the appearance of Hox-3.1 transcripts apparently follows Hox-1.5 after a small delay. Hox-3.1 and Hox-1.5 transcripts occupy domains which are different, although overlapping, along the anteroposterior axis of the embryo. The domains are first established within the ectoderm and mesoderm germ layers at days, but subsequently they persist within the nervous system, the prevertebral column and within at least some of the organs (the thyroid, lung, stomach, mesonephric and metanephric kidneys) at days. In discussion, two different mechanisms are considered by which positional information might first be generated within the germ layers.
Regional expression, pattern and timing of convergence and extension during gastrulation ofXenopus laevis
Authors: Keller Ray , Danilchik Mike 
Published: April 2021

AbstractWe show with time-lapse micrography that narrowing in the circumblastoporal dimension (convergence) and lengthening in the animal-vegetal dimension (extension) of the involuting marginal zone (IMZ) and the noninvoluting marginal zone (NIMZ) are the major tissue movements driving blastopore closure and involution of the IMZ during gastrulation in the South African clawed frog, Xenopus laevis. Analysis of blastopore closure shows that the degree of convergence is uniform from dorsal to ventral sides, whereas the degree of extension is greater on the dorsal side of the gastrula. Expiants of the gastrula show simultaneous convergence and extension in the dorsal IMZ and NIMZ. In both regions, convergence and extension are most pronounced at their common boundary, and decrease in both animal and vegetal directions. Convergent extension is autonomous to the IMZ and begins at stage 10·5, after the IMZ has involuted. In contrast, expression of convergent extension in the NIMZ appears to be dependent on basal contact with chordamesoderm or with itself. The degree of extension decreases progressively in lateral and ventral sectors. Isolated ventral sectors show convergence without a corresponding degree of extension, perhaps reflecting the transient convergence and thickening that occurs in this region of the intact embryo. We present a detailed mechanism of how these processes are integrated with others to produce gastrulation. The significance of the regional expression of convergence and extension in Xenopus is discussed and compared to gastrulation in other amphibians.
Development and characterization of commissural interneurones in the spinal cord of Xenopus laevis embryos revealed by antibodies to glycine
Authors: Roberts Alan , Dale N. , Ottersen O. P. , Storm-Mathisen J. 
Published: April 2021

Abstract
By using an antibody to glutaraldehyde fixation products of glycine we have been able to observe the development of a defined population of spinal interneurones in the CNS of Xenopus laevis embryos. The first glycine immunoreactive (GLY) somata appeared at stage 22 in the caudal hindbrain within a few hours of neural tube closure. The population then increased by extending caudally into the spinal cord and by infill. It was followed up to the time of hatching, stage 37/38. By observing GLY cells at early stages in their differentiation, the normal sequence of cell process formation was deduced. A ventral axon is formed, extends dendrites laterally into the marginal zone and forms a commissure by growing through the ventral ependymal cell floor of the neural tube. On the opposite side, growth cones turn longitudinally and TEM observations show that they make en-passant synaptic contacts. All GLY cells have decussating axons and some grow secondary axons on the same side as the soma.
To establish the identity of GLY cells, a detailed comparison was made with commissural and dorsolateral commissural interneurones defined by retrograde and intracellular HRP staining. The GLY cells are identified with the commissural interneurones which are known to serve a glycinergic reciprocal inhibitory function. By showing that these interneurones have a clearly defined group identity and programme of development, this study opens the way to further experiments on factors controlling spinal cord pathway determination.