bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplex real-time RT-PCR method for the diagnosis of SARS-CoV-2 by targeting viral N2, RdRP and human RP genes
Authors: Tombuloglu Huseyin , Sabit Hussein , Al-Suhaimi Ebtesam , Al-Khallaf Hamoud , Kabanja Juma , Al-Saleh Najat 
Published: March 2021

Abstract
Corona Virus Disease 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This pandemic has brought the world to a standstill and threatened human lives. Many methods are known to date to detect this virus. Due to their relative accuracy, polymerase chain reaction (PCR)-based assays are the most frequently applied and considered the gold standard. However, some of these assays have the disadvantages of taking time to show the result and might produce false-negative and false-positive ones. Therefore, designing rapid and accurate PCR-based testing assay is of paramount importance for early detection of this virus and for more efficient control of the spread of this disease. We, here, describe a fast, reliable, easy-to- use, and high-throughput multiplex SARS-CoV-2 RT-PCR detection method. The assay was designed to detect two viral genes (N2 and RdRP) and a human gene (RP) simultaneously. The performance and the accuracy of the assay was tested in 28 SARS-CoV-2 positive samples and compared with commercial kits, which showed 100% positive percent agreement with a limit of detection (LOD) value of 1.25 copies/µL or 5 copies/reaction. The current assay is found accurate, reliable, simple, sensitive, and specific. It can be used as an optimized SARS-CoV-2 diagnostic assay in hospitals, medical centers, and diagnostic laboratories as well as for research purposes.
Transcriptomic convergence despite genomic divergence drive field cancerization in synchronous squamous tumors
Authors: Ong Chin-Ann Johnny , Shannon Nicholas B , Lim Weng Khong , Tan Qiu Xuan , Yap Daniel R.Y. , Lek Sze Min , Tan Joey W.S. , Tan Shih Jia J. , Hendrikson Josephine , Ng Wai Har , Liu Ying , Ng Gillian , Koh Kelvin K.N. , Ng Cedric C.Y. , Rajasegaran Vikneswari , Ong Choon Kiat , Lim Tony K.H , Teh Bin Tean , Kon Oi Lian , Chia Claramae S. , Soo Khee Chee , Iyer N. Gopalakrishna 
Published: March 2021

Abstract
Field cancerization is suggested to arise from imbalanced differentiation in individual basal progenitor cells leading to clonal expansion of mutant cells that eventually replace the epithelium, although without evidence. Through deep sequencing analyses, we characterized the genomic and transcriptomic landscapes of field change in two patients with synchronous aerodigestive tract tumors. Our data support the emergence of numerous genetic alterations in cancer-associated genes but refutes the hypothesis that founder mutation(s) underpin this phenomenon. Instead, our analyses suggest a common etiologic factor defined by mutational signatures and/or transcriptomic convergence, which could provide a therapeutic opportunity.
The impacts&nbsp;of social capital, migration range and regional economic development level&nbsp;on the internal migrants'&nbsp;utilization of public health services
Authors: Yang Zhen , Jiang Chenghua 
Published: March 2021

Abstract
Background

It has been a risk of the internal migrants' (IMs') health that their utilization of National Essential Public Health Services (NEPHS) was inadequate. Studies have analyzed the impact of migration range (MR) or regional economic development level (REDL) on the IMs' utilization of NEPHS, but no studies have explored the interaction of MR and REDL on it.
Methods

Data from the China Migrant Dynamic Survey of 2017, involving 122656 IMs. Per capita GDP was set as the indicator for REDL, and all provinces were divided into three groups according to REDL: affluent, medium and poor. The MR was divided into inter-province and intra-province, and social capital (SC) was distinguished into cognitive (CSC) and structural social capital (SSC). Awareness of NEPHS (ANEPHS) and establishment of health record (EHR) were selected as indexes of NEPHS utilization. Then we used multiple line charts and hierarchical logistic regression to investigate the interaction of MR, REDL and SC on NEPHS utilization.
Results

(1) The socioeconomic status (SES) and social capital (SC) of the inter-provincial IMs were significantly lower than those of the intra-provincial IMs, and the gap was most prominent in the affluent areas. (2) From low to high, the NEPHS utilization of inter-provincial IMs was ranked as affluent, medium and poor, while the corresponding order of intra-provincial IMs was medium, poor and affluent areas. (3) SC could significantly promote the IMs' utilization of NEPHS, but there was a gap between the inter-provincial and intra-provincial IMs, and the gap was the largest in the affluent areas.
Conclution:

The IMs' SES, SC and NEPHS utilization were influenced by MR and REDL. The gaps of SES, SC and NEPHS utilization between the inter-provincial and the intra-provincial IMs in affluent areas were much larger than that in medium and poor areas. The government should pay more attention to the inter-provincial IMs in affluent areas.
Lipidomic profiling reveals distinct differences in plasma lipid composition in overweight or obese adolescent students
Authors: Yin Ruili , Wang Xiaojing , Li Kun , Yu Ke , Yang Longyan 
Published: March 2021

Abstract
Introduction

: The relationship between dyslipidemia and obesity has been widely reported, but the global lipid profiles associated with the development of obesity still need to be clarified. An investigation into the association between the lipidomic plasma profile and adolescent obesity may provide new insights into the development of obesity.
Methods

Mass spectrometry coupled with liquid chromatography was applied to detect the global lipidome in the fasting plasma from 90 Chinese adolescents, including 34 obese adolescents, 26 overweight adolescents, and 30 adolescents with a normal body mass index (BMI). All participants underwent anthropometric measurements by using InBody. Clinical biochemical indicators were measured by Cobas Elecsys.
Results

Both qualitative and quantitative analyses revealed a gradual change in plasma lipid features among obese students, exhibiting characteristics close to overweight students, but differing significantly from normal students. Compared with normal and overweight students, levels of triglyceride (TG), 18-hydroxycortisol, isohumulinone A, and 11-dihydro-12-norneoquassin were up-regulated in the obese group, while phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysoPC (LPC), lysoPE (LPE), and phosphatidylinositol (PI) were significantly down-regulated in the obese group. Then, we conducted Venn diagrams and selected 8 significant metabolites from the 3 paired comparisons. Most of the selected features were significantly correlated with the anthropometric measurements.
Conclusions

This study demonstrated evidence for a relationship between the eight significant metabolites with obese adolescents. These lipid features may provide a basis for evaluating risk and monitoring the development of obesity.
Systems analysis–complex interactions of factors that influence the sustainability of access to improved water services in a rural municipality in South Africa
Authors: Sambo Calvin , Senzanje Aidan , Mutanga Onisimo 
Published: March 2021

AbstractLimited understanding of the complex interactions of factors that influence the sustainability of access to improved water services poses a threat to rural livelihoods. The study assessed the complex interactions of the factors using a network approach. The assessment was to understand the complex interaction of the factors resulting from their cause-effect relationship that influence the sustainability of access to improved water services in a rural municipality of South Africa. The results highlighted that limited budget, limited or no water supply and improper operation and maintenance were critical factors resulting from the cause-effect relationship of other factors that influenced the sustainability of access to improved water services in the study area. We conclude that critical factors with a cause-effect relationship can influence the sustainability of access to improved water services. As a result, this deprives rural communities of the benefits derived from accessing improved water services. We recommend the use of the findings by the relevant authorities responsible for water services provision to inform planning, management policies to address challenges identified to contribute to sustainability of access to improved water services.
Self-Sampling for SARS-CoV-2 Diagnostic Testing by Using Nasal and Saliva Specimens: Protocol for Usability and Clinical Evaluation
Authors: Majam Mohammed , Msolomba Vanessa , Scott Lesley , Stevens Wendy , Marange Fadzai , Kahamba Trish , Venter Francois , Conserve Donaldson Fadael 
Published: March 2021

Background
SARS-CoV-2 is a novel coronavirus discovered in December 2019 and is currently the cause of the global COVID-19 pandemic. A critical aspect of fighting this pandemic is to obtain accurate and timely test results so that patients who have tested positive for COVID-19 can be identified and isolated to reduce the spread of the virus. Research has shown that saliva is a promising candidate for SARS-CoV-2 diagnostics because its collection is minimally invasive and can be reliably self-administered. However, little research has been conducted on saliva testing and SARS-CoV-2 self-sampling (SARS-CoV-2SS) in Sub-Saharan Africa.

Objective
The primary objective of this study is to comparatively evaluate the clinical sensitivity and specificity of nasal and oral samples self-collected by individuals for SARS-CoV-2 testing against a reference method involving sample collection and testing by a health care professional. The secondary objectives of this study are to evaluate the usability of nasal self-sampling and saliva self-sampling as a sample collection method for SARS-CoV-2 diagnostic testing by using failure mode and error assessment.

Methods
Participants will be recruited from the general population by using various methods, Participants will be screened progressively as they present at the clinical trial sites as well as in primary health care catchment areas in the inner city of Johannesburg, South Africa. In the event that recruitment numbers are low, we will use a mobile van to recruit participants from outlying areas of Johannesburg. We aim to enroll 250 participants into this study in approximately 6 weeks. Two sample types—a self-administered nasal swab and a self-administered saliva sample—will be collected from each participant, and a health care professional will collect a third sample by using a nasopharyngeal swab (ie, the standard reference method).

Results
This protocol has been approved by the University of the Witwatersrand Human Research Ethics Committee on July 31, 2020 (Protocol number EzCov003). As of May 13, 2021, 120 participants have been enrolled into the study.

Conclusions
SARS-CoV-2SS may offer many benefits to individuals, by allowing for initial self-identification of symptoms and collection of samples without involving third parties and potential risk of infection provided the sample can be safely processed via a collection system. The results of this study will provide preliminary data on the acceptability, feasibility, and usability of SARS-CoV-2SS among the general population for its future implementation.

International Registered Report Identifier (IRRID)
DERR1-10.2196/24811
Penerapan Literasi Sains di Kelas IV Sekolah Dasar
Authors: Nuro Falistya Roisatul Mar'atin , Suwandayani Beti Istanti , Majid Intan Nurul 
Published: March 2021

Hasil PISA tahun 2018 telah diumumkan oleh The Organisation for Economic Co-operation and Development (OECD). Pengukuran PISA dilakukan di Indonesia dengan melibatkan 12.098 peserta didik yang tersebar di 399 sekolah. Data tersebut menunjukkan tingkat literasi peserta didik masih rendah. Literasi yang rendah berkontribusi terhadap rendahnya produktivitas negara yaitu jumlah output yang dihasilkan dalam suatu periode. Penelitian ini bertujuan untuk menganalisis: (1) implementasi literasi sains kelas IV sekolah dasar, (2) faktor pendukung dalam pelaksanaan literasi sains kelas IV di sekolah dasar, dan (3) faktor penghambat dalam pelaksanaan literasi sains kelas di sekolah dasar. Penelitian ini menggunakan metode penelitian kualitatif dengan jenis penelitian deskriptif. Hasil penelitian menunjukkan: (1) terlaksananya literasi sains di kelas IV, (2) faktor pendukung dalam gerakan literasi sains yaitu SDM (guru kelas, orang tua peserta didik, kepala sekolah, dan warga sekolah) dan fasilitas yang tersedia di sekolah (perputakaan sekolah, referensi buku bacaan di sekolah, dan pojok baca), dan (3) faktor penghambat dalam gerakan literasi sains yaitu kurangnya dukungan beberapa orang tua peserta didik dan kurangnya minat baca peserta didikHasil PISA tahun 2018 telah diumumkan oleh The Organisation for Economic Co-operation and Development (OECD). Pengukuran PISA dilakukan di Indonesia dengan melibatkan 12.098 peserta didik yang tersebar di 399 sekolah. Data tersebut menunjukkan tingkat literasi peserta didik masih rendah. Literasi yang rendah berkontribusi terhadap rendahnya produktivitas negara yaitu jumlah output yang dihasilkan dalam suatu periode. Penelitian ini bertujuan untuk menganalisis: (1) implementasi literasi sains kelas IV sekolah dasar, (2) faktor pendukung dalam pelaksanaan literasi sains kelas IV di sekolah dasar, dan (3) faktor penghambat dalam pelaksanaan literasi sains kelas di sekolah dasar. Penelitian ini menggunakan metode penelitian kualitatif dengan jenis penelitian deskriptif. Hasil penelitian menunjukkan: (1) terlaksananya literasi sains di kelas IV, (2) faktor pendukung dalam gerakan literasi sains yaitu SDM (guru kelas, orang tua peserta didik, kepala sekolah, dan warga sekolah) dan fasilitas yang tersedia di sekolah (perputakaan sekolah, referensi buku bacaan di sekolah, dan pojok baca), dan (3) faktor penghambat dalam gerakan literasi sains yaitu kurangnya dukungan beberapa orang tua peserta didik dan kurangnya minat baca peserta didik
TAHAPAN-TAHAPAN DALAM PROSES PENGEMBANGAN KOLEKSI (Studi Kasus Perpustakaan Universitas Gajah Putih Takengon, Kabupaten Aceh Tengah)
Authors: Ifka Bengi. MS Nuri 
Published: March 2021

Collection development is a core activity that has the task of holding and expanding collections in terms of collections in a library. The purpose of developing a collection is to expand and obtain a up-to-date collection. The University of Gajah Putih Takengon Aceh Tengah Library is a private university library that functions as an information institution to support the implementation of the Tri Dharma Perguruan Tinggi. In supporting the implementation of the Tri Dharma Perguruan Tinggi and fulfilling the information needs of its users, libraries need to develop collections and formulate collection development policies. This paper examines the stages of the collection development process at the Gajah Putih Takengon University Library Aceh Tengah regency and the collection development policy. This research uses qualitative methods with a descriptive approach. Data collection obtained through observation, interviews and literature study. The results showed that the stages of the collection development process at the Gajah Putih Takengon University Library Aceh Tengah regency were carried out simply without using a written collection development policy. In addition, the minimum number of librarians and budget is a logical factor for libraries in carrying out collection development activities.
Validez del hallazgo de nódulos en la colonoscopia para el diagnóstico de hiperplasia nodular linfoide en niños
Authors: Sempértegui Cárdenas Pablo Xavier , Cadena León José Francisco , Ignorosa Arellano Karen Rubí , Toro Monjaraz Erick Manuel , Zárate Mondragón Flora Elva , Cervantes Bustamante Roberto , Ramírez Mayans Jaime Alfonso , Corcuera Delgado Celso Tomás , Becerril Cholula Laura 
Published: March 2021

Introducción: la hiperplasia nodular linfoide del colon se define como > 10 nódulos linfoides visibles en colonoscopia. No existen estudios de su validez al compararlo con la histopatología.
Objetivos: determinar la validez del hallazgo de nódulos en colonoscopia para el diagnóstico de hiperplasia nodular linfoide.
Material y métodos: estudio prospectivo de prueba diagnóstica. Se incluyeron colonoscopias realizadas consecutivamente de 2014 al 2018 con equipos Olympus PCFQ150AI y GIFXP150N con obtención de biopsias. El criterio endoscópico fue la presencia de > 10 nódulos de 2 a 10 mm y el criterio histológico fue hiperplasia de folículos linfoides y mantos de linfocitos en lámina propia o submucosa. Los datos se analizaron en Epidat3.1. Se obtuvo la sensibilidad (S), especificidad (E), valor predictivo positivo (VPP) y negativo (VPN), y coeficientes de probabilidad positivo (LR+) y negativo (LR-) con sus intervalos de confianza.
Resultados: se incluyeron 327 colonoscopias, la mediana de edad fue de 84 meses. La principal indicación para la colonoscopia fue sangrado digestivo bajo (38,8 %). El hallazgo de nódulos se encontró en el 21 % y el sitio de mayor frecuencia fue el colon total (46 %), mientras que por histopatología se encontró hiperplasia nodular linfoide en el 38 %. El hallazgo de nódulos obtuvo una S de 32 % (intervalo de confianza [IC] del 95 %: 24-140), E de 84 % (IC 95 %: 79-89), VPP de 56 % (IC 95 %: 44-68), VPN de 67 % (IC 95 %: 61-72), LR+ de 2,04 (IC 95 %: 1,4-3) y LR- de 0,8 (IC 95 %: 0,8-0,9).
Conclusiones: la validez diagnóstica del hallazgo de nódulos en colonoscopia para hiperplasia nodular linfoide es pobre, por lo que la toma de biopsia debe recomendarse siempre.
Descripción clínica y epidemiológica de pacientes con pancreatitis crónica en un hospital de alta complejidad en Cali, 2011 a 2017
Authors: Sepúlveda-Copete Mauricio , Satizabal-Padridin Natalia , Hidalgo-Cardona Alejandra , Criollo-Gutiérrez Brian D. , Tobón-Guevara Angélica , Castro-Llanos Andrés M. , Aguirre-Rojas Marisol , Rojas Rodríguez Carlos Arturo 
Published: March 2021

Introducción: la pancreatitis crónica (PC) es una inflamación progresiva del páncreas que puede llevar a su destrucción e insuficiencia irreversibles. Es una patología desafiante para el médico, debido a que establecer su diagnóstico puede tomar meses e incluso años, el seguimiento de pacientes suele ser problemático y el conocimiento sobre la clínica y epidemiología en Colombia es incompleto. Este estudio pretende hacer una descripción de pacientes con PC en un centro de referencia en gastroenterología de Cali, Colombia.
Metodología: estudio de corte transversal de pacientes adultos con PC confirmada por criterios clínicos y radiológicos entre 2011 y 2017.
Resultados: se incluyeron 36 pacientes con PC. La mayoría fueron hombres (72,2 %) y la media de edad fue 56 (+ 15,1) años. El dolor abdominal crónico fue la presentación clínica más común (83,3 %). Cerca de un cuarto de pacientes presentaba diabetes mellitus (22,2 %). Se consideró etiología idiopática en 58,3 %, alcohólica en el 11,0 % y biliar en el 11,0 %. La tomografía axial computarizada (TAC), resonancia magnética (RMN) y colangiopancreatografía por RMN fueron los métodos diagnósticos más usados (60,5 %), con los que en su mayoría se visualizó atrofia (53,1 %), dilatación de conductos (49,0 %) y calcificaciones del páncreas (34,7 %).
Conclusión: los síntomas inespecíficos de la PC en fases iniciales y su largo curso clínico favorecen al subdiagnóstico de esta patología. Los resultados presentados pueden contribuir a la futura creación de escalas clínicas locales que orienten estudios radiológicos y genéticos tempranos, con el fin de lograr un diagnóstico oportuno y mejorar la calidad de vida de estos pacientes.
Health Care Providers’ Utilization of and Perspectives on Mobile Health Technology for Diabetes and Pregnancy Support
Authors: Jackson Jenise , Leziak Karolina , Niznik Charlotte M. , Yee Lynn M. 
Published: March 2021

OBJECTIVE
Mobile health (mHealth) technology can be an effective tool to deliver behavioral interventions to improve health outcomes for individuals with diabetes. However, there is limited evidence on mHealth for pregnant women managing diabetes or for the role of health care providers (HCPs) in delivering mHealth tools. To prepare for the development of a pregnancy-specific mHealth intervention for diabetes, we sought to understand HCPs’ acceptance, utilization, and design recommendations for how mHealth technology can best be used to support the management of diabetes during pregnancy.

METHODS
Focus groups and individual interviews were conducted with clinical HCPs (obstetricians, nurses, diabetes educators, dietitians, and lactation counselors) who care for low-income pregnant women with diabetes. Data were analyzed using the constant comparative method.

RESULTS
Participants (n = 29) generated five themes and 14 subthemes. Knowledge and acceptance themes included utilization of mobile resources and barriers to HCPs’ adoption. Knowledge of popular pregnancy applications (apps) dominated, while familiarity with and use of diabetes apps varied by profession and proximity to continued education. Technology adoption barriers included lack of credibility, dependence on Internet resources, and reliance on knowledge networks. Design themes addressed user-centered design, responsive and practical education, and clinical integration. Clinicians recommended patient-participatory design, customizable features, data-tracking features, motivational feedback, and bidirectional communication capabilities.

CONCLUSION
Although HCPs strongly embraced mHealth tools as an adjunct to obstetric care for women with diabetes, they also expressed limited knowledge and apprehension about patient engagement. HCP recommendations and concerns must critically inform the development of meaningful tools for this population.
PENGARUH METODE PEMBELAJARAN MIND MAPPING DAN MOTIVASI BELAJAR TERHADAP KETERAMPILAN BERPIKIR KREATIF PESERTA DIDIK PADA MATERI INVERTEBRATA
Authors: Novitasari W.F. , Widowati Hening , Achyani Achyani 
Published: March 2021

Penelitian bertujuan mengetahui: (1) Pengaruh metode pembelajaran mindmapping‎ terhadap keterampilan berpikir ‎kreatif peserta didik pada materi Invertebrata (2) Pengaruh motivasi belajar peserta didik ‎terhadap keterampilan berpikir ‎kreatif peserta ‎didik pada materi Invertebrata (3) Interaksi antara metode pembelajaran mind mapping dan motivasi ‎‎belajar yang berbeda terhadap kemampuan ‎berpikir ‎kreatif peserta didik pada materi Invertebrata. Rancangan penelitian yang digunakan dalam penelitian ini adalah rancangan penelitian eksperimen semu atau quasi eksperiment. Desain yang digunakan adalah Pre Test-Post Test Nonequivalent Control Group Design. Populasi dalam penelitian ini adalah semua peserta didik Kelas X MIPA di SMA Negeri 6 Metro Tahun Pelajaran 2019/2020 yang terdiri dari tiga kelas. Sampel dalam penelitian ini sebanyak dua kelas. Setiap pembelajaran diwakili oleh satu kelas. Kelas X MIPA 1 sebagai kelas eksperimen metode mind mapping, dan Kelas X MIPA 2 sebagai kelas kontrol metode konvensional. Jumlah peserta didik Kelas X MIPA 1 adalah 28, Kelas X MIPA 2 berjumlah 26 peserta didik. Hasil analisis menunjukkan: (1) Terdapat pengaruh yang signifikan terkait metode pembelajaran mind mapping‎ terhadap keterampilan berpikir ‎kreatif peserta didik pada materi Invertebrata. (2) Tidak terdapat pengaruh yang signifikan terkait motivasi belajar peserta didik ‎terhadap keterampilan berpikir ‎kreatif peserta ‎didik pada materi Invertebrata. (3) Tidak terdapat interaksi antara metode pembelajaran mind mapping dan motivasi ‎‎ belajar yang berbeda terhadap kemampuan ‎berpikir ‎kreatif peserta didik pada materi Invertebrata.