bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paternal genetic structure of Kyrgyz ethnic group in China revealed by high‐resolution Y‐chromosome STRs and SNPs
Authors: Song Feng , Song Mengyuan , Luo Haibo , Xie Mingkun , Wang Xindi , Dai Hao , Hou Yiping 
Published: June 2021

AbstractKyrgyz ethnic group is one of the nomads in China, with the majority in Xinjiang and a small part of them living in Heilongjiang province. Historically, they have went through five migrations westward due to the wars. The name “Kyrgyz” means 40 tribes, originating from the primary groups of Kyrgyz. However, it is a largely understudied population, especially from the Y chromosome. In this study, we used a previously validated high‐resolution Y‐chromosome single nucleotide polymorphisms (Y‐SNPs) and short tandem repeats (Y‐STRs) system to study Kyrgyz ethnic group. A total of 314 male samples of Kyrgyz ethnic group were genotyped by 173 Y‐SNPs and 27 Y‐STRs. After data analysis, the results unveiled that Kyrgyz ethnic group was a population with high percentage of both haplogroup C2a1a3a1d∼‐F10091 (91/134) and R1a1a1b2a2‐Z2124 (109/134), which has never been reported. This implied that Kyrgyz ethnic group might have gone through bottleneck effects twice, with these two main lineages left. Mismatch analysis indicated that the biggest mismatch number in haplogroup C2a1a3a1d∼‐F10091 was 10, while that of haplogroup R1a1a1b2a2‐Z2124 was 20. This huge difference reflected the different substructure in two lineages, suggesting that haplogroup C2a1a3a1d∼‐F10091 might have the least admixture compared to the other two lineages. After admixture modelling with other datasets, the conclusion could be drawn that Kyrgyz ethnic group had great genetic affinity with Punjabi from Lahore, Pakistan, which supported that Kyrgyz ethnic group in China was close to central Asian.
Synthesis of coimmobilized microorganisms for the removal of cadmium from cadmium‐contaminated rice flour
Authors: Zhao Fang , Zhang Hu , Yan Pianpian , Chen Yuwei , Wu Qian , Fang Min , Wu Yongning , Gong Zhiyong 
Published: June 2021

AbstractChina has the greatest rice production in the world, but the problem of heavy metal pollution in rice is becoming increasingly serious. The present study examined a microbial immobilization method to remove cadmium (Cd) in rice flour. The study demonstrated that Lactobacillus plantarum (L. plantarum) exhibited the best removal effect, but the microorganisms were difficult to separate from rice flour. Diatomaceous earth coimmobilized microbial pellets (DECIMPs) were prepared using coimmobilized L. plantarum with sodium alginate (SA, 3%), polyvinyl alcohol (PVA, 2%), and diatomaceous earth (DE, 1%). Compared with microbial fermentation, the immobilized pellets had less influence on rice quality, and Cd removal rates of sample 1 (0.459 ± 0.006 mg/kg) and 2 (0.873 ± 0.031 mg/kg) reached 90.01% ± 1.01% (0.051 ± 0.003 mg/kg) and 91.80% ± 0.54% (0.068 ± 0.034 mg/kg), which were significantly higher than free microbial fermentation. In addition, microbial was easily separated. These results show that DECIMPs fermentation is an effective means of removing Cd from rice and could be considered as a strategy for the development of Cd‐free rice‐based foods.
Age‐related and individual variations in altered prefrontal and cerebellar connectivity associated with the tendency of developing internet addiction
Authors: Patil Abhishek Uday , Madathil Deepa , Huang Chih‐Mao 
Published: June 2021

AbstractInternet addiction refers to problematic patterns of internet use that continually alter the neural organization and brain networks that control impulsive behaviors and inhibitory functions. Individuals with elevated tendencies to develop internet addiction represent the transition between healthy and clinical conditions and may progress to behavioral addictive disorders. In this network neuroscience study, we used resting‐state functional magnetic resonance imaging (rs‐fMRI) to examine how and whether individual variations in the tendency of developing internet addiction rewire functional connectivity and diminish the amplitude of spontaneous low‐frequency fluctuations in healthy brains. The influence of neurocognitive aging (aged over 60 years) on executive‐cerebellar networks responsible for internet addictive behavior was also investigated. Our results revealed that individuals with an elevated tendency of developing internet addiction had disrupted executive‐cerebellar networks but increased occipital‐putamen connectivity, probably resulting from addiction‐sensitive cognitive control processes and bottom‐up sensory plasticity. Neurocognitive aging alleviated the effects of reduced mechanisms of prefrontal and cerebellar connectivity, suggesting age‐related modulation of addiction‐associated brain networks in response to compulsive internet use. Our findings highlight age‐related and individual differences in altered functional connectivity and the brain networks of individuals at a high risk of developing internet addictive disorders. These results offer novel network‐based preclinical markers of internet addictive behaviors for individuals of different ages.
Brentuximab vedotin consolidation therapy after autologous stem‐cell transplantation in patients with high‐risk Hodgkin lymphoma: Multicenter retrospective study
Authors: Akay Olga Meltem , Ozbalak Murat , Pehlivan Mustafa , Yildiz Birol , Uzay Ant , Yigenoglu Tugce Nur , Elverdi Tugrul , Kaynar Leylagul , Ayyildiz Orhan , Yonal Hindilerden Ipek , Goksoy Hasan Sami , Izmir Guner Sebnem , Gunes Ahmet Kursad , Sonmez Mehmet , Kurt Yuksel Meltem , Civriz Bozdag Sinem , Ozkurt Zubeyde Nur , Toptas Tayfur , Dogu Mehmet Hilmi , Salim Ozan , Saydam Guray , Yavasoglu Irfan , Ayli Meltem , Ozet Gulsum , Albayrak Murat , Birtas Atesoglu Elif , Toprak Selami K. , Yildirim Rahsan , Mehtap Ozgur , Kalayoglu Besisik Sevgi , Nalcaci Meliha , Altuntas Fevzi , Ferhanoglu Burhan 
Published: June 2021

AbstractThe AETHERA trial reported an increased progression‐free survival (PFS) when brentuximab vedotin (BV) was used as maintenance therapy in high‐risk Hodgkin lymphoma (HL) after autologous stem cell transplantation (ASCT). Thus, we aimed to determine the impact and safety of BV as maintenance after ASCT in real‐world patients. Seventy‐five patients with relapsed/refractory HL started on BV consolidation therapy after ASCT due to high risk of relapse, between January 2016 and July 2019, from 25 institutions, were included in the study. The median follow‐up time was 26 months. The most common high‐risk features were primary refractory or relapsed disease <12 months (n = 61), lack of complete response (CR) to the last salvage regimen (n = 51), and having had at least two salvage regimens (n = 29). At the time of analysis, 42 patients completed consolidation courses, and BV was discontinued in 33 patients. Fifty patients had an ongoing response (CR in 41, PR in 6, and SD in 3 patients), 25 had progressed. Ten died in the follow‐up, eight with progressive disease and two due to infection while in CR. The 2‐year PFS and OS rates were 67.75% (95% confidence interval [CI]: 0.55–0.77) and 87.61% (95% CI: 0.76–0.94), respectively. Seventeen patients (23%) received BV in the pre‐ASCT treatment lines, and there was no survival difference between the BV‐naïve and BV‐exposed groups. The most common adverse events were neutropenia (27%) and peripheral neuropathy (21%). Sixteen patients (21.3%) experienced grade 3 or 4 toxicity. BV was discontinued due to adverse event in 12 patients. Consolidation with BV after ASCT can achieve a 2‐year PFS of 67.75% (95% CI: 0.55–0.75) with an acceptable toxicity profile.
Effects of mental health training on capacity, willingness and engagement in peer‐to‐peer support in rural New South Wales
Authors: Maddox Sarah , Powell Nicholas N. , Booth Angela , Handley Tonelle , Dalton Hazel , Perkins David 
Published: June 2021

AbstractIssue addressedRural Australians experience significant barriers in accessing mental health services, some of which may be overcome by increasing mental health literacy in rural communities. This paper evaluates Mental Health Support Skills (MHSS), short training courses developed by the Rural Adversity Mental Health Program (RAMHP). MHSS was designed to build the capacity of community members and gatekeepers to identify people with mental health concerns and link them to appropriate resources or services.MethodsProgram data from April 2017 to March 2020 were analysed to assess the reach and outcomes of MHSS training. Training feedback was collected through a post‐training survey, completed directly after courses, and a follow‐up survey two months after training. An app used by RAMHP coordinators (the trainers) recorded the geographic and demographic reach of courses.ResultsMHSS was provided to 10,208 residents across rural New South Wales. Survey participation was 49% (n = 4,985) for the post‐training survey and 6% (n = 571), for the follow‐up survey, two months post‐training. The training was well‐received and increased the mental health understanding and willingness to assist others of most respondents (91%‐95%). Follow‐up survey respondents applied learnings to assist others; 53% (n = 301) asked a total of 2,252 people about their mental health in the two months following training. Those in clinical roles asked a median of 6 people about their mental health, compared to 3 for those in nonclinical roles. Most follow‐up survey respondents (59%, n = 339) reported doing more to look after their own mental health in the two months after training.ConclusionThese results are encouraging as they suggest that short‐form mental health training can be an effective tool to address poorer mental health outcomes for rural residents by improving the ability of participants to help themselves and the people around them.So what?Serious consideration should be given to short mental health courses, such as MHSS, to increase literacy and connection to services, especially in rural areas.
Thinking of future as an older individual increases perceived risks for age‐related diseases but not for COVID‐19
Authors: Monzani Dario , Marinucci Marco , Pancani Luca , Rusconi Patrice , Mazzoni Davide , Pravettoni Gabriella 
Published: June 2021

Actively thinking of one's future as an older individual could increase perceived risk and risk aversion. This could be particularly relevant for COVID‐19, if we consider the common representation of the risk of being infected by COVID‐19 as associated with being older. Increased perceived risk could bear consequences on the adoption of preventive behaviours. Thus, we investigated whether increasing the salience of individuals' future as an older adult would impact on their perceived risk for COVID‐19 and medical conditions varying for age‐relatedness. One hundred and forty‐four Italian adults (Mage = 27.72, range: 18–56) were randomly assigned to either a future as older adult thinking or control condition. Perceived risk for COVID‐19 and other strongly, and weakly age‐related medical conditions during the lifetime was measured. Results showed that thinking about the future as an older adult increased perceived risk for strongly and weakly age‐related diseases, but not for COVID‐19. The salience of the COVID‐19 outbreak may have raised the perceived risks in both experimental conditions, making the manipulation ineffective. In conclusion, manipulating future‐oriented thinking might be a successful communication strategy to increase people's perceived risk of common diseases, but it might not work for highly salient pathologies such as COVID‐19.
Searching for local order parameters to classify water structures at triple points
Authors: Doi Hideo , Takahashi Kazuaki Z. , Aoyagi Takeshi 
Published: June 2021

AbstractThe diversity of ice polymorphs is of interest in condensed‐matter physics, engineering, astronomy, and biosphere and climate studies. In particular, their triple points are critical to elucidate the formation of each phase and transitions among phases. However, an approach to distinguish their molecular structures is lacking. When precise molecular geometries are given, order parameters are often computed to quantify the degree of structural ordering and to classify the structures. Many order parameters have been developed for specific or multiple purposes, but their capabilities have not been exhaustively investigated for distinguishing ice polymorphs. Here, 493 order parameters and their combinations are considered for two triple points involving the ice polymorphs ice III‐V‐liquid and ice V‐VI‐liquid. Supervised machine learning helps automatic and systematic searching of the parameters. For each triple point, the best set of two order parameters was found that distinguishes three structures with high accuracy. A set of three order parameters is also suggested for better accuracy.
Psychologist homicide victims: The National Violent Death Reporting System and other sources
Authors: Robiner William N. , Li Tiffany 
Published: June 2021

AbstractObjective: Homicide of healthcare workers has been reported but little is known about psychologists as victims of homicide. This study aimed to investigate what is known about homicide of psychologists. Methods: The Centers for Disease Control and Prevention's National Center for Injury Prevention and Control's National Violent Death Reporting System (NVDRS) provides insights into violent deaths, including homicide. This study interrogated the NVDRS and conducted Internet searches about psychologist decedents of homicide. Results: Between 2003 and 2018 the NVDRS identified 12 psychologist homicides. Internet searches revealed 15 psychologist homicides. Only three (11.1%) were known to have been killed by current or former patients. Another was by a colleague's patient. Another assailant was suspected to be a patient. We summarize patterns and circumstances of deaths, address issues related to violence in healthcare, and review selected resources addressing violence prevention. Conclusion: Psychologists, like other health professionals, may be victims of homicide. Such deaths have diverse contributing factors and are rarely perpetrated by patients. As concern about health professional safety mounts and the NVDRS becomes better established, it will hopefully become a more sensitive, precise, comprehensive and useful mechanism for tracking trends in violent deaths of psychologists and other health professionals and ultimately inform preventative strategies.
Cognizance of posttranslational modifications in vaccines: A way to enhanced immunogenicity
Authors: Ojha Rupal , Prajapati Vijay Kumar 
Published: June 2021

AbstractVaccination is a significant advancement or preventative strategy for controlling the spread of various severe infectious and noninfectious diseases. The purpose of vaccination is to stimulate or activate the immune system by injecting antigens, i.e., either whole microorganisms or using the pathogen's antigenic part or macromolecules. Over time, researchers have made tremendous efforts to reduce vaccine side effects or failure by developing different strategies combining with immunoinformatic and molecular biology. These newly designed vaccines are composed of single or several antigenic molecules derived from a pathogenic organism. Although, whole‐cell vaccines are still in use against various diseases but due to their ineffectiveness, other vaccines like DNA‐based, RNA‐based, and protein‐based vaccines, with the addition of immunostimulatory agents, are in the limelight. Despite this, many researchers escape the most common fundamental phenomenon of protein posttranslational modifications during the development of vaccines, which regulates protein functional behavior, evokes immunogenicity and stability, etc. The negligence about post translational modification (PTM) during vaccine development may affect the vaccine's efficacy and immune responses. Therefore, it becomes imperative to consider these modifications of macromolecules before finalizing the antigenic vaccine construct. Here, we have discussed different types of posttranslational/transcriptional modifications that are usually considered during vaccine construct designing: Glycosylation, Acetylation, Sulfation, Methylation, Amidation, SUMOylation, Ubiquitylation, Lipidation, Formylation, and Phosphorylation. Based on the available research information, we firmly believe that considering these modifications will generate a potential and highly immunogenic antigenic molecule against communicable and noncommunicable diseases compared to the unmodified macromolecules.
Stimulation of the four isoforms of receptor tyrosine kinase ErbB4, but not ErbB1, confers cardiomyocyte hypertrophy
Authors: Wang Zhen , Chan Hsiu‐Wen , Gambarotta Giovanna , Smith Nicola J. , Purdue Brooke W. , Pennisi David J. , Porrello Enzo R. , O'Brien Shannon L. , Reichelt Melissa E. , Thomas Walter G. , Paravicini Tamara M. 
Published: June 2021

AbstractEpidermal growth factor (EGF) receptors (ErbB1–ErbB4) promote cardiac development and growth, although the specific EGF ligands and receptor isoforms involved in growth/repair versus pathology remain undefined. We challenged ventricular cardiomyocytes with EGF‐like ligands and observed that selective activation of ErbB4 (the receptor for neuregulin 1 [NRG1]), but not ErbB1 (the receptor for EGF, EGFR), stimulated hypertrophy. This lack of direct ErbB1‐mediated hypertrophy occurred despite robust activation of extracellular‐regulated kinase 1/2 (ERK) and protein kinase B. Hypertrophic responses to NRG1 were unaffected by the tyrosine kinase inhibitor (AG1478) at concentrations that are selective for ErbB1 over ErbB4. NRG1‐induced cardiomyocyte enlargement was suppressed by small interfering RNA (siRNA) knockdown of ErbB4 and ErbB2, whereas ERK phosphorylation was only suppressed by ErbB4 siRNA. Four ErbB4 isoforms exist (JM‐a/JM‐b and CYT‐1/CYT‐2), generated by alternative splicing, and their expression declines postnatally and following cardiac hypertrophy. Silencing of all four isoforms in cardiomyocytes, using an ErbB4 siRNA, abrogated NRG1‐induced hypertrophic promoter/reporter activity, which was rescued by coexpression of knockdown‐resistant versions of the ErbB4 isoforms. Thus, ErbB4 confers cardiomyocyte hypertrophy to NRG1, and all four ErbB4 isoforms possess the capacity to mediate this effect.
Defects of cohesin loader lead to bone dysplasia associated with transcriptional disturbance
Authors: Gu Weihuai , Wang Lihong , Gu Renjie , Ouyang Huiya , Bao Baicheng , Zheng Liwei , Xu Baoshan 
Published: June 2021

AbstractCohesin loader nipped‐B‐like protein (Nipbl) is increasingly recognized for its important role in development and cancer. Cornelia de Lange Syndrome (CdLS), mostly caused by heterozygous mutations of Nipbl, is an autosomal dominant disease characterized by multiorgan malformations. However, the regulatory role and underlying mechanism of Nipbl in skeletal development remain largely elusive. In this study, we constructed a Nipbl‐a Cas9‐knockout (KO) zebrafish, which displayed severe retardation of global growth and skeletal development. Deficiency of Nipbl remarkably compromised cell growth and survival, and osteogenic differentiation of mammalian osteoblast precursors. Furthermore, Nipbl depletion impaired the cell cycle process, and caused DNA damage accumulation and cellular senescence. In addition, nucleolar fibrillarin expression, global rRNA biogenesis, and protein translation were defective in the Nipbl‐depleted osteoblast precursors. Interestingly, an integrated stress response inhibitor (ISRIB), partially rescued Nipbl depletion‐induced cellular defects in proliferation and apoptosis, osteogenesis, and nucleolar function. Simultaneously, we performed transcriptome analysis of Nipbl deficiency on human neural crest cells and mouse embryonic fibroblasts in combination with Nipbl ChIP‐Seq. We found that Nipbl deficiency caused thousands of differentially expressed genes including some important genes in bone and cartilage development. In conclusion, Nipbl deficiency compromised skeleton development through impairing osteoblast precursor cell proliferation and survival, and osteogenic differentiation, and also disturbing the expression of some osteogenesis‐regulatory genes. Our study elucidated that Nipbl played a pivotal role in skeleton development, and supported the fact that treatment of ISRIB may provide an early intervention strategy to alleviate the bone dysplasia of CdLS.
Over 1‐year duration and age difference of SARS‐CoV‐2 antibodies in convalescent COVID‐19 patients
Authors: Zeng Fanfan , Wu Mengjun , Wang Jinbiao , Li Jianyu , Hu Guoyun , Wang Lin 
Published: June 2021

AbstractAnti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) immunoglouilin G (IgG) and immunoglouilin M (IgM) antibodies have been widely used to assist clinical diagnosis. Our previous study reported a discrepancy in SARS‐CoV‐2 antibody response between male and female coronavirus disease 2019 (COVID‐19) patients. However, the duration and discrepancy between ages as well as sexes of SARS‐CoV‐2 antibody in convalescent COVID‐19 patients have not been clarified. In this study, a total of 538 health‐examination individuals who were confirmed with SARS‐CoV‐2 infection a year ago were enrolled. Blood samples were collected and detected for IgM and IgG antibodies. Among these convalescent patients, 12.80% were detected positive for IgM antibodies. The positive rates for IgM antibody were close between sexes: for males, this is 9.17% and for females 13.75%. However, the IgG antibody was detected positive in as much as 82.90% convalescent patients and the positive rates were nearly the same between males (82.57%) and females (82.98%). Besides this, the level of IgM and IgG antibodies showed no difference between male and female convalescent patients. The level of IgG antibodies showed a significant difference between ages. The elder patients (over 35 years old) maintained a higher level of IgG antibody than the younger patients (under or equal 35 years old) after recovering for 1 year. In addition, IgG antibody was more vulnerable to disappear in younger patients than in elder patients. Overall, our study identified over 1‐year duration of SARS‐CoV‐2 antibody and age difference of IgG antibody response in convalescent COVID‐19 patients. These findings may provide new insights into long‐term humoral immune response, vaccines efficacy and age‐based personalized vaccination strategies.