bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of a Molecular Beacon Probe–Based Real-Time Polymerase Chain Reaction Assay for Rapid Detection of Methicillin Resistance in Staphylococcus aureus
Authors: Elsayed Sameer , Chow Barbara L. , Hamilton Nina L. , Gregson Daniel B. , Pitout Johann D. D. , Church Deirdre L. 
Published: January 2021

Abstract
Context.—A rapid, real-time, duplex, fluorescent molecular beacon probe–based polymerase chain reaction (PCR) assay was recently developed for the detection of methicillin-resistant Staphylococcus aureus.
Objective.—To describe the development and validation of this unique assay.
Design.—Prospective laboratory analysis.
Setting.—Urban health region/centralized diagnostic microbiology laboratory.
Bacterial Strains.—One hundred eighty-one previously characterized clinical and American Type Culture Collection isolates, including 50 strains each of methicillin-resistant and methicillin-sensitive S aureus, plus 50 strains of coagulase-negative staphylococci and 31 nonstaphylococcal isolates to ensure assay specificity.
Intervention.—Assays were performed on purified genomic DNA extracted from growing bacterial colonies. Two sets of oligonucleotide primers were used to specifically amplify the mecA and nuc genes, followed by detection of amplicons using fluorophore-labeled molecular beacon probes. Assays were performed on the Mx4000 Multiplex Quantitative PCR System (Stratagene Inc, La Jolla, Calif).
Main Outcome Measures.—(1) Assay sensitivity and specificity, and (2) analytical sensitivity.
Results.—The assay demonstrated 100% sensitivity and 100% specificity, and accurately characterized isolates as methicillin-resistant S aureus, methicillin-sensitive S aureus, or methicillin-resistant coagulase-negative staphylococci, with test results available in 2.5 hours. The analytical sensitivity of the assay was determined to be between 6 and 60 genomic equivalents.
Conclusions.—This assay is rapid, accurate, easy to perform, and is compatible with other real-time PCR instruments, making it a suitable alternative to conventional PCR methodologies.
Left-Sided Splenorenal Fusion With Marked Extramedullary Hematopoiesis and Concurrent Lithium Toxicity
Authors: Yuan Shan , Vaughan Matthew , Nicholas Agoff S. 
Published: January 2021

Abstract
Occasionally, heterotopic splenic tissue can occur in the renal fossa secondary to splenosis following splenic trauma or splenectomy. More rarely, it can represent a developmental anomaly secondary to the fusion of splenic and renal tissues. Splenorenal fusion can present as a renal mass, mimicking primary or secondary renal neoplasms on imaging studies, and patients can also present with symptoms of hypersplenism (anemia). We report a case of splenorenal fusion in a 51-year-old woman who initially presented with lithium toxicity, anemia, thrombocytosis, and a large renal mass that mimicked a primary renal neoplasm. The possible embryologic origin of splenorenal fusion, effects of lithium toxicity, and utility of various imaging modalities are discussed. The literature on renal heterotopic splenic tissue is also briefly reviewed.
Anaplastic Large Cell Lymphoma Arising in a Silicone Breast Implant Capsule: A Case Report and Review of the Literature
Authors: Sahoo Sunati , Rosen Paul P. , Feddersen Richard M. , Viswanatha David S. , Clark Douglas A. , Chadburn Amy 
Published: January 2021

Abstract
Anaplastic large cell lymphoma is a rare type of primary breast lymphoma. We report a case of anaplastic large cell lymphoma, T-cell phenotype, occurring in the periprosthetic capsule of a silicone breast prosthesis 9 years after implantation for augmentation mammoplasty. This case is unique for its unusual presentation.
Diffuse Neuroendocrine Differentiation in a Morphologically Composite Mammary Infiltrating Ductal Carcinoma: A Case Report and Review of the Literature
Authors: David Odile , Bhattacharjee Meena 
Published: January 2021

Abstract
Neuroendocrine differentiation has been reported in both in situ and infiltrating breast cancers. The prognostic significance of neuroendocrine differentiation in mammary carcinoma is unclear. We report a case of infiltrating ductal carcinoma in which there was a morphologically conventional-appearing infiltrating ductal component admixed with nests of cells that resembled a carcinoid tumor and initially mimicked the appearance of intraductal carcinoma. Immunohistochemical stains for synaptophysin and chromogranin demonstrated diffuse, strong positivity uniformly throughout the tumor, even in the more conventional-appearing areas. Electron microscopic examination of tissue retrieved from paraffin blocks was attempted unsuccessfully. We concluded that this was an infiltrating ductal carcinoma with morphologic and immunohistochemical evidence of neuroendocrine differentiation. The case is discussed with a review of the literature and a discussion of nomenclature for tumors of the breast showing variable degrees of neuroendocrine differentiation.
Duodenal Gangliocytic Paraganglioma With Lymph Node Metastasis: A Case Report and Review of the Literature
Authors: Sundararajan Vanitha , Robinson-Smith Toni M. , Lowy Andrew M. 
Published: January 2021

Abstract
A case of duodenal gangliocytic paraganglioma (DGP) in a 67-year-old woman is presented. The DGP arose in the second part of the duodenum. Although most of the reported cases of DGP are considered benign, in the present case, we found regional lymph nodes containing metastatic tumor. Previous reports have documented metastases containing only epithelioid cells. The current case demonstrates metastatic tumor in regional lymph nodes containing all 3 of the DGP components (spindle cells, ganglion-like cells, and epithelioid cells).
Natural Killer–like T-Cell Lymphoma of the Small Intestine With a Distinct Immunophenotype and Lack of Association With Gluten-Sensitive Enteropathy
Authors: Yuan Constance M. , Stein Steven , Glick John H. , Wasik Mariusz A. 
Published: January 2021

Abstract
We report the case of a large natural killer (NK)–like T-cell lymphoma that involved the ileum and displayed a distinct immunophenotype and complex karyotype. The patient exhibited no evidence of gluten-sensitive enteropathy (celiac disease) or any other type of enteropathy as determined by clinical history, endoscopy, and serology for immunoglobulin A (IgA) antiendomysial and IgG antigliadin antibodies. Molecular studies demonstrated a clonal T-cell receptor γ chain gene rearrangement. Immunophenotype analysis showed expression of intestinal epithelium-homing receptor CD103, CD7, cytoplasmic CD3ɛ, CD56, and CD16 but no other T- or NK-cell markers. Cytogenetic analysis of the malignant cells revealed multiple chromosomal abnormalities indicative of a biologically advanced, high-grade lymphoma. A novel subset of normal intestinal intraepithelial lymphocytes, bearing a similar phenotype, has been described; moreover, this subset diminishes, rather than expands, in gluten-sensitive enteropathy. This case supports the notion that lymphomas involving the small intestine represent a heterogeneous group of lymphomas with diverse pathogenetic mechanisms.
Infection byDipylidium caninumin an Infant
Authors: Molina Claudia P. , Ogburn James , Adegboyega Patrick 
Published: January 2021

AbstractDipylidium caninum, the dog tapeworm, is a cosmopolitan parasite of dogs and cats and occasionally causes human infection in the United States. Diagnosis is made by observing the characteristic rice grain–like proglottids in stool specimens and the pathognomonic egg packets in the gravid uterus in histologic sections of the parasite. There have been few reported cases of human infection with this parasite, and very little information on the pathology of this zoonotic disease is available in the English language. This report of a case of D caninum infection in a 6-month-old infant highlights the diagnostic features of this disease. To our knowledge, this is the first case to be reported in the American pathology literature during the last 36 years (MEDLINE database, 1966–2002).
Rosai-Dorfman Disease
Authors: Cocker Rubina S. , Kang John , Kahn Leonard B. 
Published: January 2021

AbstractTo the best of our knowledge, this is the only reported case of isolated involvement by Rosai-Dorfman disease (RDD) of small, anterior cervical-midline lymph nodes, clinically presenting as a thyroid mass. Thyroid parenchymal involvement by RDD has been reported in only 3 cases in the literature. The present case shows involvement of RDD of a pretracheal and thyroid isthmic lymph node in a 38-year-old woman. The progressively enlarging, anterior neck mass was diagnosed as “lymph node” on a fine-needle aspiration biopsy specimen and subsequently interpreted to be an isthmic cyst on ultrasonography. A magnetic resonance imaging scan revealed foci of nodularity in the thyroid isthmus and pretracheal lymph node. Excisional biopsy of the 2 masses revealed typical features of sinus histiocytosis with massive lymphadenopathy in the lymph node. Review of the patient's previous fine-needle aspiration biopsy specimens also revealed the presence of similar features. Currently, the patient is well and has no other manifestation or recurrence of RDD.
Mediastinal Epithelioid Solitary Fibrous Tumor
Authors: Marchevsky Alberto M. , Varshney Dolly , Fuller Clark 
Published: January 2021

Abstract
We describe the case of a 74-year-old man with a mediastinal tumor composed predominantly of epithelioid cells exhibiting histopathologic and immunohistochemical features intermediate between those of a solitary fibrous tumor and those of a cellular adenomatoid tumor. We discuss the differential diagnosis and possible histogenesis of this unusual neoplasm, and we propose the term epithelioid solitary fibrous tumor for this entity.
Plasma Cell Granuloma—An Enigmatic Lesion
Authors: Buccoliero Anna Maria , Caldarella Adele , Santucci Marco , Ammannati Franco , Mennonna Pasquale , Taddei Antonio , Taddei Gian Luigi 
Published: January 2021

Abstract
We present an unusual case of intracranial plasma cell granuloma in a 70-year-old man with a 6-month history of progressive visual disturbance. The lesion extensively involved the cranial base, extended into the frontal region, and reached the floor of the third ventricle in the suprasellar area. Microscopic examination of multiple diagnostic transsphenoidal biopsies showed an inflammatory proliferation with a predominance of cells that were immunohistochemically determined to be polyclonal plasma cells. Ultrastructural analysis confirmed the presence of numerous mature plasma cells in a mixed inflammatory proliferation. In situ hybridization for Epstein-Barr viral RNA revealed no evidence of viral expression. The patient was treated with steroid therapy and radiotherapy, without any appreciable reduction of the lesion's size. He is alive with persistent severe visual disturbance 14 months after the diagnosis. We discuss the etiopathogenetic, diagnostic, and therapeutic issues related to this entity, and review the literature.
Fulminant Adenovirus Hepatitis Following Bone Marrow Transplantation
Authors: Wang Wei Hua , Wang Hanlin L. 
Published: January 2021

Abstract
Adenovirus infections are frequently encountered in immunocompromised patients and transplant recipients. However, fulminant hepatic failure due to adenovirus infection as a fatal complication in bone marrow transplant recipients is extremely rare. We report a case of fulminant adenovirus hepatitis in a 21-year-old allogeneic bone marrow transplant recipient who subsequently died of the disease. The diagnosis was established by histologic and immunohistochemical examination of a liver biopsy and was confirmed by liver tissue and blood cultures.
Two Cases of Epithelioid Angiosarcoma Involving the Thyroid and a Brief Review of Non-Alpine Epithelioid Angiosarcoma of the Thyroid
Authors: Goh Seng Geok Nicholas , Chuah Khoon Leong , Goh Hood Keng Christopher , Chen Yun Yin Cosmas 
Published: January 2021

Abstract
Epithelioid angiosarcoma involving the thyroid is a rare entity, more often described in the Alpine region. Two cases of epithelioid angiosarcoma that affected the thyroid in patients from a non-Alpine location were diagnosed during a 10-year period in our department. The first case occurred in an 89-year-old Chinese man with a history of longstanding goiter, whereas the second case involved a 74-year-old Chinese man with a history of angiosarcoma of the scalp. On histologic examination, both thyroid tumors were composed of plump epithelioid cells with vesicular chromatin and prominent nucleoli, forming vascular structures and solid sheets. Positive staining for CD31 and factor VIII–related antigen confirmed endothelial differentiation in both cases. Both patients died within 5 months following the diagnosis of thyroid disease. The relationship of the scalp angiosarcoma and thyroid disease of the second patient is unclear. A brief review of non-Alpine primary thyroid epithelioid angiosarcoma is presented.
Rosai-Dorfman Disease Presenting in the Gastrointestinal Tract
Authors: Anders Robert A. , Keith Jeanette Newton , Hart John 
Published: January 2021

Abstract
Rosai-Dorfman disease (RDD) rarely presents a diagnostic challenge. However, unusual sites of presentation can cause confusion. We present a case of RDD of the colon in a symptomatic 51-year-old woman. The gastrointestinal tract is the organ system least commonly affected by RDD, with only 11 cases reported. Most cases have been found incidentally or at autopsy. The widespread use of endoscopic procedures makes it more likely that this entity will be encountered. Most patients with gastrointestinal tract involvement have evidence of widespread extranodal disease and have a protracted clinical course.
Involvement of Meckel Diverticulum in Crohn Disease Associated With Pancreatic Heterotopia
Authors: Mourra Najat , Tiret Emmanuel , Caplin Scott , Gendre Jean-Pierre , Parc Rolland , Flejou Jean-Francois 
Published: January 2021

Abstract
Although the association of Crohn disease and Meckel diverticulum has been widely reported, the direct involvement of a Meckel diverticulum by Crohn disease is less common and is usually the result of contiguous spread. The presence of heterotopic tissues in an affected diverticulum is rare and most often consists of gastric mucosa. We report herein the presence of Crohn disease within a Meckel diverticulum, with a normal small bowel immediately adjacent to the diverticulum, associated with pancreatic heterotopia.
Transcriptome analysis reveals liver metabolism programming in kids from nutritional restricted goats during mid-gestation
Authors: Yang Chao , Zhou Xiaoling , Yang Hong , Gebeyew Kefyalew , Yan Qiongxian , Zhou Chuanshe , He Zhixiong , Tan Zhiliang 
Published: January 2021

Background
Maternal nutrient restriction during pregnancy causes a metabolic disorder that threatens the offspring’s health in humans and animals. However, the molecular mechanism of how undernutrition affecting hepatic metabolism of fetal or postnatal offspring is still unclear. We aimed to investigate transcriptomic changes of fetal livers in response to maternal malnutrition in goats during mid-gestation and to explore whether these changes would disappear when the nutrition was recovered to normal level during mid-gestation using goats (Capra hircus) as the experimental animals.

Methods
Fifty-three pregnant goats were subjected to a control (100% of the maintenance requirements, CON) or a restricted (60% of the maintenance requirements on day 45 to day 100 of gestation and then realimentation, RES) diet. A total of 16 liver samples were collected from fetal goats on day 100 of gestation and goat kids of postnatal day 90 to obtain hepatic transcriptional profiles using RNA-Seq.

Results
Principal component analysis of the hepatic transcriptomes presented a clear separation by growth phase (fetus and kid) rather than treatment. Maternal undernutrition up-regulated 86 genes and down-regulated 76 genes in the fetal liver of the FR group as compared to the FC group. KEGG pathway analysis showed the DEGs mainly enriched in protein digestion and absorption, steroid biosynthesis, carbohydrate digestion and absorption and bile secretion. A total of 118 significant DEGs (fold change > 1.2 and FDR < 0.1) within KR vs. KC comparison was identified with 79 up-regulated genes and down-regulated 39 genes, and these DEGs mainly enriched in the biosynthesis of amino acids, citrate cycle, valine, leucine and isoleucine biosynthesis and carbon metabolism.

Conclusion
Hepatic transcriptome analysis showed that maternal undernutrition promoted protein digestion and absorption in the fetal livers, while which restrained carbohydrate metabolism and citric acid cycle in the livers of kid goats after realimentation. The results indicate that maternal undernutrition during mid-gestation causes hepatic metabolism programming in kid goats on a molecular level.
ATR-FTIR, EDS and SEM evaluations of enamel structure after treatment with hydrogen peroxide bleaching agents loaded with nano-hydroxyapatite particles
Authors: Orilisi Giulia , Tosco Vincenzo , Monterubbianesi Riccardo , Notarstefano Valentina , Özcan Mutlu , Putignano Angelo , Orsini Giovanna 
Published: January 2021

Background
Tooth whitening is one of the most requested dental treatments, but it still presents some side effects. Indeed, the bleaching agent can generate patients’ discomfort and dental hard tissue damages, not achieving an efficient and long-lasting treatment with optimum whitening effect. To overcome these limitations, the bleaching agents containing nano-hydroxyapatite can represent a reliable solution to avoid these detrimental effects.

Methods
In this study, human third molars were treated with commercial bleaching agents, containing nano-hydroxyapatite (nHA) and 6% (at-home treatment), 12% and 18% (in-office treatments) of hydrogen peroxide (HP), named respectively G1, G2 and G3. The results were evaluated descriptively and analytically using Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray spectroscopy (EDS), comparing the treated groups with a commercial gel containing 10% carbamide peroxide (CONV) and with a non-treated control group (CTRL).

Results
ATR-FTIR analysis revealed a similar composition in carbonates and phosphates for CTRL, G1 and G2 groups, which showed no significant differences in their spectral profiles; an increased amount of organic matter was found in G3, while CONV displayed an intermediate behavior. SEM analysis did not highlight significant changes in the enamel microstructure of G1 and CONV when compared to CTRL; the pattern observed in G2 presented a slight increase of enamel irregularities, while G3 displayed a partial removal of the aprismatic layer and microporosities. No evident effects due to nHA were observed in the structure of the hydroxyapatite component of G1, G2 and CONV, if compared to CTRL, while G3 showed a slight loss of crystallinity. In all groups, EDS identified slight changes in the concentration of chemical elements O and Ca, while the Ca/P ratio was similar when compared to CTRL.

Conclusion
The obtained results suggest that the application of the tested commercial bleaching agents, with a concentration of HP up to 12%, does not alter the morphological and chemical composition of the enamel surface and maintains its crystallinity.
Occupation and life satisfaction among individuals with mental illness: the mediation role of self-reported psychophysiological health
Authors: Granjard Alexandre , Mihailovic Marko , Amato Clara , Kazemitabar Maryam , Lucchese Franco , Jacobsson Christian , Kijima Nobuhiko , Garcia Danilo 
Published: January 2021

BackgroundUnemployment can diminish physical, psychological and social health. In this context, research shows that people with mental illness have even more difficulties finding occupation. Thus, some countries, such as Sweden, strive after creating job opportunities for this specific group. We investigated the effect of having an occupation on life satisfaction among individuals with mental illness and whether self-reported physical and psychological health mediated the relationship between being (un)employed and life satisfaction.MethodTwo-hundred eighty-seven individuals (148 males, 134 females, and 5 missing information) with mental illness, who received support and services from Swedish Municipalities in Blekinge, self-reported occupation, life satisfaction, and physical and psychological health.ResultsParticipants who reported having an occupation reported also significantly higher levels of life satisfaction, physical health, and psychological health compared to those without occupation. Nevertheless, these differences were rather small (Eta2< 0.06). Moreover, the indirect effect of having an occupation on life satisfaction through physical and psychological health was significant. Finally, the total indirect effect of physical and psychological health (i.e., psychophysiological health) accounted for 53% of the total effect of having an occupation on life satisfaction.ConclusionFor individuals with mental illness there seems to be an almost equal importance of indirect and direct effects of having an occupation on their levels of life satisfaction. More specifically, while there are differences in life satisfaction within this population in relation to having an occupation, having an occupation leads to the sense of good psychophysiological health, which in turn helps individuals with mental illness to feel satisfied with their lives.
Some bryophytes newly reported for the Australian continent
Authors: Van Zanten Ben , During Heinjo , Sollman Philippus 
Published: January 2021

Anoectangium euchloron (Schwägr.) Mitt., Campyliadelphus chrysophyllus (Brid.) R.S. Chopra and Tortula
caucasica Broth. are reported as new for the Australian continent.
Ti‐Based Surface Integrated Layer and Bulk Doping for Stable Voltage and Long Life of Li‐Rich Layered Cathodes
Authors: Luo Dong , Cui Jiaxiang , Zhang Bingkai , Fan Jianming , Liu Peizhi , Ding Xiaokai , Xie Huixian , Zhang Zuhao , Guo Junjie , Pan Feng , Lin Zhan 
Published: January 2021

AbstractHigh‐energy‐density lithium‐rich layered oxides (LLOs) hold the greatest promise to address the range anxiety of electric vehicles. Their application, however, has been prevented by fast voltage decay and capacity fading for years, which mainly originate from irreversible transition‐metal migration and undesirable cathode‐electrolyte interfacial reactions. Herein, a Ti‐based surface integrated layer and bulk doping, which greatly improve the voltage and capacity stability of LLOs is synchronously constructed. More importantly, STEM and Raman results demonstrate that continuous and uniform surface Ti‐based integrated layer is a spinel‐like rocksalt structure with Fd‐3m space group, which is built through by several the replacement of Li ions in surface several atomic layers by Ti ions. After 500 cycles, Ti‐150 sample delivers a capacity retention of 85%, and its voltage decay rate from the 30th to the 500th cycle is only ≈0.72 mV/cycle. Spectral results and DFT calculations suggest that bulk Ti‐doping mitigates the migration of Mn and Ni ions in the bulk, while Ti‐based integrated layer significantly suppresses surface structure evolution and interfacial reactions by impeding the generation of surface Li vacancies during Li extraction as well as preventing direct contact between electrolyte and active materials.