bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-directed highly para-selective C-H functionalization of TIPS-protected phenols promoted by a proton shuttle
Authors: Geng Jingyao , Fang Zhang , Tu Guangliang , Zhao Yingsheng 
Published: December 2021

Abstract
Palladium-catalyzed non-directed C-H functionalization provides an efficient approach for direct functionalization of arenes, but it usually suffers from poor site selectivity, limiting its wide application. Herein, it is reported for the first time that the proton shuttle of 3,5-dimethyladamantane-1-carboxylic acid (1-DMAdCO2H) can affect the site selectivity during the C-H activation step in palladium-catalyzed non-directed C-H functionalization, leading to highly para-selective C-H olefination of TIPS-protected phenols. This transformation displayed good generality in realizing various other para-selective C-H functionalization reactions such as hydroxylation, halogenation, and allylation reactions. A wide variety of phenol derivatives including bioactive molecules of triclosan, thymol, and propofol, were compatible substrates, leading to the corresponding para-selective products in moderate to good yields. A preliminary mechanism study revealed that the spatial repulsion factor between proton shuttle and bulky protecting group resulted in the selective C-H activation at the less sterically hindered para-position. This new model non-directed para-selective C-H functionalization can provide a straightforward route for remote site-selective C-H activations.
New Physics II: Spin Picture, Particle Structure, and Fundamental Interactions
Authors: Kang China 
Published: December 2021

Abstract
Experimental data sometimes fails to render the expected truth, such as high-speed bullets smashing into pieces on a water surface cannot verify the water’s hardness. By re-examining the essence underneath quantum phenomena and analyzing their relevance to universal classical theory, this study has thoroughly revealed the classical counterpart of spin. Subsequently, the equivalence between spin angular momentum (of energy or charge) and vorticity flux (of energy or charge) has also been unveiled, thus intuitively clarifying many abstruse physical concepts, like spin magnetic moment, virtual electron, relativistic time dilation, neutrino chirality, quark origin, and fundamental interactions (including gravitons). From now on, almost all quantum puzzles (e.g., wave-particle duality, quantum entanglement, Schrödinger’s cat) can be understood classically, just as prominent physicists such as Planck, Einstein, and Schrödinger longed for back then. This paper can be considered a blueprint of the Theory of Everything (TOE).
The Practice of Indigenous Education for Students with Disabilities in the Selected Church Schools of South Gondar Administrative Towns-Ethiopia
Authors: Asres Getachew Walelign 
Published: December 2021

Abstract
The main objective of this study is to assess the practice of indigenous education for students with disabilities in Ethiopian indigenous schools. Therefore, 20 Orthodox Church schools were purposefully selected in order to make them representatives of South Gondar Administrative towns. The result of the study showed that there was no ability difference between students with disabilities and students without disabilities in the different levels of the church schools(X 2 =0.62 and its Chi-square per item is 1.509, .521, .036, 1.393, .158, .015, .793, .100, and 1.050 at df =1, p =.220, .471, .850, .238, .691, .902, .373, .751 and .305 >0.05 from items 1-9 respectively). In addition, church scholars could not use special assessment techniques for students with disabilities in the different church schools. Inaccessible environment is the major challenge that impede the educational performance of students with disabilities. Therefore, students with disabilities need different support services from stakeholders.
Australian General Practitioner Perceptions to Sharing Clinical Data for Secondary Use: A Mixed Method Approach
Authors: Varhol Richard J , Randall Sean , Boyd James H , Robinson Suzanne 
Published: December 2021

Abstract
ObjectiveThe potential for data collected in general practice to be linked and used to address health system challenges of maintaining quality care, accessibility and safety, including pandemic support, has led to an increased interest in public acceptability of data sharing, however practitioners have rarely been asked to share their opinions on the topic. This paper attempts to gain an understanding of general practitioners’ perceptions on routinely sharing practice data for both population health planning and healthcare research both from an Australian and international perspective.Materials and MethodsA mixed methods approach combining an initial online survey followed by face-to-face interviews (before and during COVID-19), designed to identify the barriers and facilitators to sharing data, were conducted on a representative sample of general practitioners across Western Australia (WA).ResultsEighty online surveys and ten face-to-face interviews with general practitioners were conducted from Nov 2020 – May 2021. Although respondents overwhelmingly identified the importance of population health research, their willingness to participate in data sharing programs was determined by a perception of trust associated with the organisation collecting and analysing shared data; a clearly defined purpose and process of collected data; including a governance structure providing confidence in the data sharing initiative simultaneously enabling a process of data sovereignty and autonomy.DiscussionResults indicate strong agreement around the importance of sharing patient’s medical data for population and health research and planning. Concerns pertaining to lack of trust, governance and secondary use of data continue to be a setback to data sharing with implications for primary care business models being raised.ConclusionTo further increase general practitioner’s confidence in sharing their clinical data, efforts should be directed towards implementing a robust data governance structure with an emphasis on transparency and representative stakeholder inclusion as well as identifying the role of government and government funded organisations, as well as building trust with the entities collecting and analysing the data.
The Next “G” On ESG: The Strategic Thinking of Businesses Towards Supply Chain Fraud
Authors: Sariannidis Nikolaos , Ragazou Konstantina , Passas Ioannis , Garefalakis Alexandros 
Published: December 2021

Abstract
Supply Chain Management is in the core of businesses’ operational activities worldwide. Its main purpose is the proper management of resources and the assurance of the sustainable operation of the economic entities. However, Supply Chain Management is exposed to breaches related to the code of conduct as well as fraud. Integrating the principles of Environmental, Social and Corporate Governance (ESG) can help build a healthy, sustainable, and resilient supply chain. The purpose of the research is twofold and refers to: (i) highlight those factors of the ESG that contribute to the decrease and mitigation of the fraud in supply chain and (ii) the business strategies that can be developed from businesses and can be based on ESG factors. In this context, a log-log model of multiple linear regression was proposed. Secondary data were extracted from the Thomson Reuters database. The model was based on 681 observations concerning companies operating in Europe. The results have led to the conclusion that the existence of policies related to human resources and technology contribute significantly to tackling supply chain fraud. Regarding the first factor, Human Resource is important to feel safe and their rights should be protected by companies. Securing their rights can lead individuals to their commitment to the work environment, as well as to their protection from threats and violations. Finally, the role of technology is fully consistent with transparency in the supply chain. For this reason, the adoption of reliable solutions and technologies, which turn to the green economy, offer visibility and optimization of processes.
Hippocampal Representations for Deep Learning on Alzheimer’s Disease
Authors: Sarasua Ignacio , Pölsterl Sebastian , Wachinger Christian 
Published: December 2021

Abstract
Deep learning offers a powerful approach for analyzing hippocampal changes in Alzheimer's disease (AD) without relying on handcrafted features. Nevertheless, an input format needs to be selected to pass the image information to the neural network, which has wide ramifications for the analysis, but has not been evaluated yet. We compare five hippocampal representations (and their respective tailored network architectures) that span from raw images to geometric representations like meshes and point clouds. We performed a thorough evaluation for the prediction of AD diagnosis and time-to-dementia prediction with experiments on an independent test dataset. Our results show that choosing an appropriate representation of the hippocampus for predicting Alzheimer's disease with deep learning is crucial, since it impacts performance and ease of interpretation.
Accelerated biological aging in people with Down syndrome with full and segmental trisomy 21 begins in childhood as revealed by immunoglobulin G glycosylation
Authors: Cindric Ana , Vuckovic Frano , Koschut David , Borelli Vincenzo , Juric Julija , Pucic-Bakovic Maja , Slana Anita , Deris Helena , Frkatović Azra , Murray Aoife , Alic Ivan , Groet Jurgen , O’Brien Niamh , Petrovic Drazen , Hamburg Sarah , Startin Carla , Hithersay Rosalyn , D’Souza Hana , Consortium LonDownS , Spector Tim , Mitrecic Dinko , Kero Mijana , Odak Ljubica , Barisic Ingeborg , Thomas Michael , Strydom Andre , Rebillat Anne-Sophie , Franceschi Claudio , Lauc Gordan , Kristic Jasminka , Nizetic Dean 
Published: December 2021

Abstract
Cells from people with Down syndrome (DS) show faster accumulation of DNA damage and epigenetic aging marks. Causative mechanisms remain un-proven and hypotheses range from amplified chromosomal instability to actions of several supernumerary chromosome 21 genes. Plasma immunoglobulin G (IgG) glycosylation profiles are established as a reliable predictor of biological and chronological aging. We performed IgG glycan profiling of n=246 individuals with DS (208 adults and 38 children) from three European populations and compared these to age-, sex- and demography-matched general populations. We uncovered very significantly increased IgG glycosylation aging marks associated with DS. Average levels of IgG glycans without galactose (G0) and those with two galactoses (G2) as a function of age in persons with DS corresponded to levels detected in 19 years older euploid individuals. Some aging marks were significant already in children with DS. Remarkably, the IgG glycan profiles of a child with segmental duplication of only 31 genes on chromosome 21 had values similar to those of age-matched DS children, outside the normal children’s range. This is the first non-epigenetic evidence of accelerated systemic biological aging in DS, suggesting it begins very early in childhood. It points to a causative contribution of the overdose of genes in a short segment of chromosome 21, not previously linked to accelerated aging, opening a route to discovery of hitherto unrecognised mechanisms.
Molecular Characterization of Parental Lines and Validation of Snp Markers for Disease Resistance in Common Bean
Authors: Gomes-Messias Lucas Matias , Vianello Rosana Pereira , Monteiro-Júnior Joney Pereira , Rodrigues Luana Alves , Mota Ana Paula Simplício , Pereira Helton Santos , Melo Leonardo Cunha , Raatz Bodo , Souza Thiago Lívio P. O. 
Published: December 2021

Abstract
The implementation of molecular tools that help the early selection of genotypes carrying target alleles increases efficiency and reduces the time and costs of breeding programs. The present study aimed the molecular characterization and validation of SNPs targeting disease resistance alleles for assisted selection. A total of 376 common bean lines with contrasting responses for anthracnose and angular leaf spot resistance were used, as well as 149 F2 plants from the cross between BRS Cometa x SEL 1308 (carrying the Anthracnose resistance gene Co-42). Seven of the ten SNP markers evaluated showed potential for assisted breeding: snpPV0025 (Phg-2), snpPV0027 (Phg-5), snpPV0079 (Phg-5), snpPV0046 (Co-u), snpPV0068 (Co-42), snpPV0070 (Co-42) and snpP8282v3-817 (Co-42). Markers snpPV0070 and snpP8282v3-817 showed high efficiency of selection (99.7 and 99.8%, respectively). These markers exhibit great potential to assist in the selection at different stages of the breeding program and may be readily incorporated into marker-assisted selection.
Genome-wide Investigation of Genetic Diversity in the Poultry Red Mite, Dermanyssus Gallinae, From European Farms Utilising a NGS-Multiplex Platform
Authors: Karp-Tatham Eleanor , Xia Dong , Nisbet Alasdair J , Mateus Teresa Letra , Tomley Fiona M. , Blake Damer P. 
Published: December 2021

Abstract
The poultry red mite (Dermanyssus gallinae), an obligatory blood feeding ectoparasite, is primarily associated with egg laying hens where it is estimated to cause losses of ~€230 million per annum from European farmers. Current control strategies, including the use of acaricidal chemicals and desiccant dusts, are often ineffective and there is widespread resistance to acaricides across Europe. Alternative methods to control D. gallinae are urgently required and strategies include development of recombinant subunit vaccines and discovery of new potential acaricides. These strategies will benefit hugely from knowledge of the extent and rates of occurrence of genetic diversity within D. gallinae populations. In this study, genetic diversity of mites harvested from the UK and from sites across mainland Europe was studied at inter- and intra-farm levels. To achieve this, the genome analysis toolkit (GATK) best practices pipeline for single nucleotide polymorphism (SNP) and insertion/deletion variant calling was modified to be self-validating and used to identify 32,599 D. gallinae SNPs by comparing transcriptomic sequences (derived from mites harvested in Germany, Schicht et al.) with a D. gallinae genome assembly (derived from mites harvested in Scotland, Burgess et al.). Dermanyssus gallinae populations were sampled from 22 UK farms and 57 farms from 15 countries in mainland Europe. Analysis of 144 high-quality SNP markers across 117 pooled D. gallinae samples showed high spatial genetic diversity with significant linkage disequilibrium. Revisiting a subset of farms revealed notable temporal changes in genetic diversity.
Reliability and Generalization of Gait Biometrics Using 3D Inertial Sensor Data and 3D Optical System Trajectories
Authors: Santos Geise , Tavares Tiago , Rocha Anderson 
Published: December 2021

Abstract
Particularities in the individuals’ style of walking have been explored for at least three decades as a biometric trait, fueling the automatic gait recognition field. Whereas, gait recognition works usually focus on improving end-to-end performance measures, and this work aims at understanding which individuals’ traces are more relevant to improve subjects’ separability. For such, a manifold projection technique and a multi-sensor gait dataset were adopted to investigate the impact of each data source characteristics on this separability. The assessments have shown it is hard to distinguish individuals based only on their walking patterns in a subject identification scenario. In this scenario, the subjects’ separability is more related to their physical characteristics than their movements related to gait cycles and biomechanical events. However, this study’s results also points to the feasibility of learning identity characteristics from individuals’ walking patterns learned from similarities and differences between subjects in a verification setup. The explorations concluded that periodic components occurring in frequencies between 6Hz and 10Hz are more significant for learning these patterns than events and other biomechanical movements related to the gait cycle, as usually explored in the literature.
Efficacy and Safety Testing of a COVID-19 Era Emergency Ventilator in a Healthy Rabbit Lung Model
Authors: White Luke A. , Maxey Benjamin S. , Solitro Giovanni F. , Takei Hidehiro , Conrad Steven A. , Alexander J. Steven 
Published: December 2021

Abstract
Background: The COVID-19 pandemic revealed a substantial and unmet need for low-cost, easily accessible mechanical ventilation strategies for use in medical resource-challenged areas. Internationally, several groups developed non-conventional COVID-19 era emergency ventilator strategies as a stopgap measure when conventional ventilators were unavailable. Here, we compared our FALCON emergency ventilator in a rabbit model and compared its safety and functionality to conventional mechanical ventilation. Methods: New Zealand white rabbits (n = 5) received mechanical ventilation from either the FALCON or a conventional mechanical ventilator (Engström CarestationTM) for 1 hour each. Airflow and pressure, blood O2 saturation, end tidal CO2, and arterial blood gas measurements were measured. Additionally, gross and histological lung samples were compared to spontaneously breathing rabbits (n = 3) to assess signs of ventilator induced lung injury.Results: All rabbits were successfully ventilated with the FALCON. At identical ventilator settings, tidal volumes, pressures, and respiratory rates were similar between both ventilators, but the inspiratory to expiratory ratio was lower using the FALCON. End tidal CO2 was significantly higher on the FALCON, and arterial blood gas measurements demonstrated lower arterial partial pressure of O2 at 30 minutes and higher arterial partial pressure of CO2 at 30 and 60 minutes using the FALCON. However, when ventilated at higher respiratory rates, we observed a stepwise decrease in end tidal CO2. Poincaré plot analysis demonstrated small but significant increases in short-term and long-term variation of peak inspiratory pressure generation from the FALCON. Wet to dry lung weight and lung injury scoring between the mechanically ventilated and spontaneously breathing rabbits were similar. Conclusions: Although conventional ventilators are always preferable outside of emergency use, the FALCON ventilator safely and effectively ventilated healthy rabbits without lung injury. Emergency ventilation using accessible and inexpensive strategies like the FALCON may be useful for communities with low access to medical resources and as a backup form of emergency ventilation.
Human Infrapatellar Fat Pad Mesenchymal Stem Cells Show Immunomodulatory Exosomal Signatures
Authors: Kouroupis Dimitrios , Kaplan Lee D , Best Thomas M 
Published: December 2021

Abstract
Within the human knee infrapatellar fat pad (IFP) and synovium, resident synoviocytes and macrophages contribute to the onset and progression of inflammatory joint diseases. Our hypothesis is that IFP-derived mesenchymal stem cells (IFP-MSC) robust immunomodulatory therapeutic effects are largely exerted via their exosomal (IFP-MSC EXOs) secretome by attenuating synoviocyte and macrophage pro-inflammatory activation. IFP-MSC EXOs showed distinct miRNA and protein immunomodulatory profiles. Reactome analysis of 24 miRNAs highly present in exosomes showed their involvement in the regulation of six gene groups, including immune system. Exosomes were enriched for immunomodulatory and reparative proteins that are involved in positive regulation of cell proliferation, response to stimulus, signal transduction, signal receptor activity, and protein phosphorylation. Stimulated synoviocytes or macrophages exposed to IFP-MSC EXOs demonstrated significantly reduced proliferation, altered inflammation-related molecular profiles, and reduced secretion of pro-inflammatory molecules compared to stimulated alone. In an acute synovial/IFP inflammation rat model, IFP-MSC EXOs therapeutic treatment resulted in robust macrophage polarization towards an anti-inflammatory therapeutic M2 phenotype within the synovium/IFP tissues. Based on these findings, we propose a viable cell-free alternative to MSC-based therapeutics as an alternative approach to treating synovitis and IFP fibrosis.
Discovery of Antimicrobials By Mex : Massively Parallelized Growth Assays
Authors: Koch Philipp , Schmitt Steven , Cardner Mathias , Beerenwinkel Niko , Panke Sven , Held Martin 
Published: December 2021

Abstract
The number of newly approved antimicrobial compounds has been steadily decreasing over the past 50 years emphasizing the need for novel antimicrobial substances. Here we present Mex, a method for the high-throughput discovery of novel antimicrobials, that relies on E.coli self-screening to determine the bioactivity of more than ten thousand naturally occurring peptides. Analysis of thousands of E. coli growth curves using next-generation sequencing enables the identification of more than 1,000 previously unknown antimicrobial peptides. Additionally, by incorporating the kinetics of growth inhibition, a first indication of the mode of action is obtained, which has implications for the ultimate usefulness of the peptides in question. The most promising peptides of the screen are chemically synthesized and their activity is determined in standardized susceptibility assays. Ten out of 15 investigated peptides efficiently eradicate bacteria at a minimal inhibitory concentration in the lower µm or upper nm range. This work represents a step-change in the high-throughput discovery of functionally diverse antimicrobials.
Ornaments are equally informative in males and females: a bivariate meta-analysis in birds
Authors: Nolazco Sergio , Delhey Kaspar , Nakagawa Shinichi , Peters Anne 
Published: December 2021

Abstract
Female ornaments are often reduced, male-like traits. Although these were long perceived as nonadaptive, it is now broadly accepted that female ornaments can be functional. However, it is unclear whether this is as common in females as it is in males, and whether ornaments fulfil similar signalling roles. To test this, we conduct a systematic review and apply a phylogenetically controlled bivariate meta-analysis to a large dataset of ornaments in mutually ornamented birds. As expected, female ornament expression tends to be reduced compared to males. However, ornaments are equally strongly associated with indicators of body condition and aspects of reproductive success in both sexes, regardless of the degree of sexual dimorphism. Thus, ornaments in birds provide similar information in both sexes: more ornamented individuals are in better condition and achieve higher reproductive success. Although limited by their correlational nature, these outcomes imply that female ornaments could widely function in a similar manner as male ornaments.