bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparative Clinical Study of Jaladhara and Taildhara in the Management of Stress
Authors: Tank Neha G 
Published: April 2021

In the present Era, stress is said to be one of the largest killers of man today. Stress is Â related to our total health - physical, mental and emotional. In Ayurveda stress is a resultant condition due to pragyaparadha. In the present study total 137 patients were registered and randomly divided into 3 groups viz: Group A were subjected to Jaladhara 7days along with Mansyadi Kwatha for 30days; Group B were subjected to Tailadhara for 7 days along with Mansyadi Kwatha for 30days; Group C Â received only Mansyadi Kwatha for 30days. The main aims & object of the study are: 1) To study the comparative effect of Jaladhara and Tailadhara in the management of stress. 2) To study the clinical efficacy of Shirodhara. Assessment of the effect of treatment was done on the basis of relief in the subjective & objective signs & symptoms of stress and on Brief psychiatric rating scale. The data were analysed statistically. It was observed that symptoms like insomnia, anxiety etc were almost completely relieved in Shirodhara group. In Breif Psychiatric rating scale statistically results of both the dhara group are highly significant. Taildhara provided better relief in all most all the symptoms of Stress and in Brief psychiatric rating scale compared to Jaladhara
Effect of Apamarga Kshara Taila Uttarbasti in the Management of Infertility w.s.r. Tubal-blockage
Authors: A Rajput Shivshankar , Shweta Mata , P Dei L , B Donga S , Nilofar Shaikh 
Published: April 2021

Infertility is the most sensitive and cumbersome problem which haunts every couple. The major cause in infertility is female factor which is 30.20%. Among female factor the sub factors are ovarian, tubal, endometrial and uterine. Female Infertility due to tubal blockage is the 2nd most contributing factor, in 30% of the cases. So for this study, we considered only tubal infertility and to make a pin-pointed assessment criteria. The criteria for selection of patients and assessment of results were unilateral or bilateral tubal blockage diagnosed in hysterosalpingography (HSG).Â Thus 18 patients in the age group of 20-40 years were registered for the study, with 75% primary infertility and 25% secondary infertility. Of these patients 16 completed the course of treatment. Patients having acute pelvic infection, hypersensitivity to chemical dye, congenital anomalies of vulva & vagina, CA cervix, STD or any debilitating diseases like T.B. were excluded. Apamagra Kshara Taila was selected for its Vata-Kapha Shamaka and Lekhana properties. The dose of Uttar Basti was 5 ml with duration of two consecutive cycles at the interval of three days. The tubal blockage was removed in 75% of the patients and 25% of the patients had conceived within the follow-up period of two months. The results suggest that Uttar Basti is a safer, cost effective and highly significant Ayurvedic treatment modality for tubal-blockage, with no apparent complications.
Prevalence and etiopathogenesis of Upapluta Yonivyapad w.s.r. Vulvovaginitis during Pregnancy: A Survey Study
Authors: Shaikh Nilofar , Rajput Shivshankar , Cholera Meera , Dei Laxmipriya , Donga Shilpa 
Published: April 2021

It is accepted by everyone that Ayurveda is an ancient most literature in medical world. In the early period of Samhita the traces of survey can be seen mainly aiming to the prevention of people suffering from various diseases. Survey is the way by which one can present the classical facts and directives in more practical manner to the current society. Upapluta Yonivyapad is one of the 20 Yonivyapad described by Acharyas. On the basis of the textual description Upapluta Yonivyapad can be compared to vulvovaginitis during pregnancy. Here, Survey study was aimed to elicit etiopathogenesis and prevalence of Upapluta Yonivyapad Â Â in and around Jamnagar city. The Study was conducted on 200 pregnant women attending the OPD of SRPT Dept. (I.P.G.T. & R.A.), Jamnagar, of whom 100% cooperated to give vaginal smear samples to microbiology laboratory. Organisms like gram negative bacteria and yeast were found in (92.5%) and (17.5%) of cases respectively. Etiological factors like Vishamashana in (49%) of cases, Divaswapa (day sleep) in (74.5%), excessive intake of Madhura Rasa in (68.5%) and Ati-Vyavaya (Excessive coitus) in (58%) were observed. Survey questionnaire shows that certain dietary and life style factors contribute towards occurrence of Upapluta Yonivyapad Â Â in maximum i.e. 56.5% of patients.
Curcumin affects the prognosis of renal cell carcinoma (RCC) through a negative feedback loop of H19/miR-675/HDAC/CTCF
Authors: Ni Wenjuan , Liu Fuhe , Yu Songlin , Li Fanzhu , Zhu Jiazhen , Chen Ziwei , Cheng Ying , Li Chaoqun 
Published: April 2021

IntroductionThe objective of this study was to explore the role of Curcumin (CUR) in the control of renal cell carcinoma (RCC) as well as the molecular mechanism underlying the effect of CUR.Material and methodsReal-time PCR, Western-blot analysis, immunohistochemistry (IHC) assay, and luciferase assay were utilized to detect the effect of CUR.ResultsMesoscale nanoparticles (particles) could be safely and preferentially accumulated in the kidney in a dose-dependent manner, and the specific localization of particles in the kidney reached its maximum level at a dose of 25 mg/kg. Treatment with CUR alleviated RCC by up-regulating the expression of H19 and miR-675 while down-regulating the expression of HDAC1 and HDAC6 in RCC rats. Furthermore, the underlying mechanism of CUR in the regulation of H19 was explored, and it was revealed that CUR increased H19 expression via increasing the translational efficiency of H19 promoter. Therefore, the treatment with CUR increased the levels of H19 and miR-675 mRNAs while reducing the expression of HDAC1 and HDAC6. According to a computational analysis, HDAC1 and HDAC6 were both direct targets downstream of miR-675, and miR-675 mimics could decrease the luciferase activity of cells transfected by wild-type HDAC1 and HDAC6 3’UTR. In addition, miR-675 but not CTCF reduced the protein levels of HDAC1 and HDAC6. Nevertheless, CTCF increased the luciferase activity of cells transfected by H19 promoter, while miR-675 mimics decreased the effect of CTCF.ConclusionsIn this study, we suggested that CUR could affect the prognosis of RCC by establishing a negative feedback loop of H19/miR-675/HDAC/CTCF.
PUMA mediated afatinib-induced apoptosis in glioma cells
Authors: Lu Rongzhong , Sun Xinyue , Ma Yaping , Wang Rong 
Published: April 2021

IntroductionAfatinib exhibits a tumor-inhibiting effect in different cancers by inducing apoptosis; however, its pro-apoptosis role in glioma cells is still not fully understood.Material and methodsIn the current study, two glioma cell lines (U373-MG and U87-MG) were treated with afatinib to measure their tolerance for afatinib-triggered cell death and apoptosis.ResultsWe found that afatinib treatment repressed both growth and proliferation and induced apoptosis in glioma cells. Moreover, it increased the expression of pro-apoptotic p53-upregulated modulator of apoptosis (PUMA). The influence of PUMA on afatinib-triggered apoptosis was assessed by PUMA overexpression and knockdown in glioma cell lines. PUMA overexpression resulted in an increased sensitivity of glioma cell lines toward afatinib, whereas its knockdown abated the effect of afatinib on apoptosis. Similarly, the in vivo potency of afatinib on U373-MG xenograft tumors in wild type (WT) and PUMA knockdown nude mice was measured. Afatinib treatment reduced the weight and volume of WT xenograft tumors but did not have the same effect on PUMA knockdown xenograft tumors. Afatinib also induced significant cell death and apoptosis in WT xenograft tumors but not in PUMA knockdown xenograft tumors.ConclusionsIn conclusion, afatinib induces apoptosis in glioma cells by mediating PUMA expression. This study warrants further investigation into the mechanism of afatinib in glioblastoma treatment.
The evaluation for CpG island methylation of FASN and SREBP promoter in Mongolian gerbils NAFLD model
Authors: Wu Jiusheng , Liu Yuehuan 
Published: April 2021

IntroductionThe methylation of CpG island in promoter and its nearby area is one of the most important way to inhibit gene expression. The aim of this study was to examine the potential relationship between the CpG island methylation for fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP-1).Material and methodsTen newborn pups of gerbils as NB group, 10 adult gerbils with normal diet as control group, 10 adults with high-fat diet as NAFLD group, and 10 8-month-old as aged group. Blood and liver samples were collected for serum lipid detection and histopathology. A pyrosequencing technique was employed to determine the methylation rate. Then, the transcription and expression level for FASN and SREBP-1 were verified.ResultsSerum chelosterol and triglyceride was significantly increased in NAFLD and aged group (vs. control, P<0.05). The gerbils in NAFLD and aged group also showed obvious hepatic steatosis confirmed by histological examination. Control group had the highest methylation rate for FASN and SREBP-1, which were reduced in NAFLD and aged group. Except for NB group, both the transcription and expression levels of SREBP-1 and FASN genes were presented control group> Aged group > NAFLD group. Genes of SREBP and FASN showed a trend of hypomethylation in the NAFLD gerbil model.ConclusionsThe expression of SREBP gene tended to decrease, while the expression of FASN gene tended to increase with the age and disease development. FASN and SREBP-1 methylation might be a new method to evaluate NAFLD animal model and an available target for genetic marker screening.
Flow cytometric characterization of cell surface markers to differentiate between fibroblasts and mesenchymal stem cells of different origin
Authors: Sober Suzanne A. , Darmani Homa , Alhattab Dana , Awidi Abdalla 
Published: April 2021

IntroductionIdentification and purification of mesenchymal stem cells (MSCs) expanded in culture for therapeutic use is crucial for improved yield and optimal results. Fibroblasts are the most common cell type in connective tissue and are commonly found as contaminants of MSC cultures, affecting cell yield and potentially causing tumour formation after cell transplantation. In the current study, we wished to identify cell surface markers that can differentiate MSCs of different origins from fibroblasts.Material and methodsMesenchymal stem cells were isolated from bone marrow, adipose tissue, Wharton’s jelly, and placental tissue, and fibroblasts were isolated from foreskin (as a negative control) in order to examine the differences in the expression of a panel of 14 different cell surface markers using multiplex flow cytometry.ResultsOur results indicate that the following markers could be useful in differentiating between fibroblasts and MSCs derived from the following: adipose tissue – CD79a, CD105, CD106, CD146, and CD271; Wharton’s jelly – CD14, CD56, and CD105; bone marrow – CD105, CD106, and CD146; and placental tissue – CD14, CD105, and CD146. Furthermore, we found that, contradictory to previous studies, CD26 is not fibroblast specific.ConclusionsThe results of our study indicate that cell surface markers may prove to be a useful tool in the discrimination between MSCs of different origins and fibroblasts, and thus may be used to authenticate the identity of the isolated cells.
Long non-coding RNA NEAT1/miR-320b/MSI2 axis regulates cisplatin resistance in ovarian cancer
Authors: Zhao Chunling , Zi Pingfen , Zhou Degang 
Published: April 2021

IntroductionOvarian cancer (OC) frequently occurs in postmenopausal women and it has higher mortality rate. Accumulating researches proved that long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) involved in the progression of chemoresistance in human OC. Here, the study aimed to investigate the partial molecular mechanism of OC chemoresistance.Material and methodsThe levels of NEAT1 and microRNA-320b (miR-320b) were measured by qRT-PCR. Western blot was carried out to determine the protein levels that used in this research. Cell viability was identified via Cell Counting Kit-8 (CCK-8). Transwell assay was employed to determine migration and invasion. The relationship between miR-320b and NEAT1 or MSI2 was clarified by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) and RNA pull down assay. Also, a murine xenograft assay was used to explore the effect of NEAT1 on cisplatin resistance in OC in vivo.ResultsThe level of NEAT1 was significantly increased in cisplatin resistant OC cell lines. Downregulation of NEAT1 enhanced cisplatin sensibility in OVCAR-3/DDP and HEY/DDP cells. Furthermore, miR-320b was a target of NEAT1, and the effects of knockdown of NEAT1 on the cell viability, IC50 of cisplatin, migration and invasion in OVCAR-3/DDP and HEY/DDP were restored by the inhibitor of miR-320. In addition, miR-320b directly targeted MSI2 to regulate cisplatin sensibility in cisplatin resistant OC cells. In addition, downregulation of NEAT1 decreased cisplatin resistance in OC in vivo.ConclusionsNEAT1 regulated cisplatin resistance through NEAT1/miR-320b/MSI2 axis in OC, which might offer a novel therapy target for the chemotherapy of OC.
Age-related differences in the prehospital management
of 2,500 patients with sudden cardiac arrest
Authors: Sielski Janusz , Kaziród-Wolski Karol , Solnica Marta , Data Mirosław , Kukla Dominika , Woźnica Kamil , Sadowski Marcin 
Published: April 2021

IntroductionPrehospital care affects outcomes after out-of-hospital cardiac arrest (OHCA). The aim of the study is to analyze age-related differences
in prehospital care and survival after OHCA and to define variables affecting
the efficacy of cardiopulmonary resuscitation (CPR).Material and methodsWe performed analysis of differences in patient
characteristics influencing the efficacy of CPR and analysis of survival in
four age groups: < 65, 65–74, 75–84, and ≥ 85. This retrospective registry-based study aimed to compare prehospital care in OHCA patients across
age groups.ResultsCPR was performed in 2,500 patients. Return of spontaneous circulation (ROSC) occurred in 1061 subjects. Of them, 339 had incomplete
medical records, 201 survived at least 24 h, 115 up to 30 days and 78 were
alive at 365 days after discharge. The occurrence of shockable rhythms and
the ROSC rate decreased with age. Overall mortality increased with age.
Such factors as age, gender, urban area, home location, time to arrival, and
witnessed OHCA were predictors of the initial shockable rhythm. Gender, urban area, OHCA witnessed by family member, time to arrival, cardiac cause
and shockable rhythm were predictors of ROSC. The risk of death increased
with each age group by about 56% (HR = 1.56, p < 0.0001).ConclusionsShockable initial rhythm and urban location were the strongest predictors of ROSC. Survival at 30 and 365 days after OHCA decreased
in older patients. Survival among older patients with OHCA is worse than
in younger subjects, which results from lower efficacy of resuscitation and
more frequent death declared upon arrival.
An updated quantitative evaluation of the association
between leukaemia risk and XRCC3 Thr241Met
polymorphism
Authors: Drokow Emmanuel , Effah Clement , Amenador Kate Benedicta , Agboyibor Clement , Akpabla Gloria , Ahmed Hafiz Abdul Waqas , Song Juanjuan , Sun Kai 
Published: April 2021

IntroductionSeveral analyses have been conducted to assess the association between leukaemia risk and XRCC3 Thr241Met polymorphism. However, their results are conflicting. Hence, this comprehensive study was carried
out to obtain a more accurate assessment of the association between leukaemia risk and XRCC3 Thr241Met (rs861539) polymorphismMaterial and methodsWe searched Ovid, Chinese Biomedical Literature
Database (CBM), China National Knowledge Infrastructure (CNKI), Google
Scholar, PubMed database and Excerpta Medica Database (EMBASE) for potential eligible studies published as of July 2020. Pooled odds ratio (OR)
and 95% confidence interval (ci) were used in evaluating the strength of
the association between Thr241Met polymorphism of XRCC3 and leukaemia, and the p-value less than 0.05 (p < 0.05) was considered as the level
of significance.ResultsA total of 17 studies consisting of 7241 controls and 4198 cases
met our inclusion criteria. No significant association was observed between
leukaemia risk and Thr241Met polymorphism of XRCC3 across the five genetic variants. In our ethnicity subgroup evaluation, we noted a significant
association between leukaemia risk and Thr241Met polymorphism of XRCC3
among Caucasians under four genetic models. Furthermore, SNP (rs861539)
of XRCC3 has a protective effect among Asians under four genetic models.ConclusionsThr241Met polymorphism of XRCC3 has a protective effect in
the Asian population but has oncogenic potential in the Caucasian population.
Study on the mechanism of multi-AGC kinase AT13148 on Notch signaling pathway in glioblastoma
Authors: Li Yanan , Han Guosheng , Min Weijie , Li Mengmeng , Wang Maomao , Chen Chao , Chen Yi , Wang Laixing , Yue Zhijian 
Published: April 2021

IntroductionGlioblastoma is the most malignant astrocytoma, and its therapeutic effect is not ideal. Notch signaling pathway plays an important role in tumor proliferation and invasion. Whether small molecule drug AT13148 can affect glioblastoma by regulating Notch signaling pathway is the focus of this study.Material and methodsIn vitro, glioblastoma U87 cell line transfected with sh-ITGB1 (U87sh-ITGB1), U87 cell line transfected with oe-ITGB1 (U87oe-ITGB1) and control group were treated with a small molecular drug AT13148. RT-qPCR, western-blot and clone formation ability assays were used to detect the mRNA and protein expression of the ITGB1 and the key gene NOTCH1, as well as the proliferation of cancer cells. Therapeutic effects of AT13148 were examined in vivo using a nude mice model of U87 cells. After treatment with AT13148, volume of tumors were calculated, and RT-qPCR and western-blot were used to evaluate the mRNA and protein expression of the ITGB1 and NOTCH1.ResultsAT13148 inhibits the activity of U87 cells. Lentiviral transfection of sh-ITGB1 and oe-ITGB1 can interfere with the expression of ITGB1 in U87 cells. AT13148 could down-regulate both the expression of ITGB1 and NOTCH1. Moreover, AT13148 affects the cloning ability of U87 cells. AT13148 can also inhibit the proliferation of U87 cells. Furthermore, AT13148 inhibited the proliferation and invasion of transplanted tumors in vivo.ConclusionsThis study indicated that AT13148 could affect the expression of ITGB1 and NOTCH1, which also could be a potential potential anti-glioblastoma small molecule drug candidate in clinic medicine.
Modelo De Sistema Viable Modelo De Articulación Del Sistema De Gestión Integral HSEQ Bajo Los Criterios De Las Normas ISO 9001:2015, ISO 14001: 2015 E ISO 45001:2018
Authors: Calderón Pino Anggie Daniela , López Mendoza Miguel Ángel , Romero Hernández María Alejandra 
Published: April 2021

El modelo de sistema viable es un modelo que busca ser implementado dentro de todas las organizaciones independientemente de su actividad; este busca generar autonomía en todas las partes de la organización a través de un modelo replicado de integración sinergia y cohesión. Este modelo se conforma por Mecanismos de cohesión en donde encontramos el canal de comandos, coordinación y el monitoreo de las actividades primarias; y el mecanismo de adaptación donde encontramos las políticas, inteligencia el control y cohesión. Este modelo de sistema viable permite una correcta comunicación entre todas las áreas de la empresa a través de interacciones entre actividades todas apuntando al mismo objetivo. Lo que se pretende realizar es implementar una lista de verificación que nos sirva como diagnostico dentro de las organizaciones e identifique las principales áreas en donde se está cumpliendo a cabalidad el sistema de modelo viable y cuales son aquellas donde presenta deficiencias.
Lo que se pretende con estos hallazgos es asegurar la integración entre todas las partes involucradas; creando sinergia y eficiencia mediante un mismo sistema viable conectado y replicado en cada área.
The Case Study of Life Cycle Perspective in Ready-mix Industry
Authors: Widowati Yosephine Dwi Martina 
Published: April 2021

ASEAN Economic Community (AEC) is a global commitment and has been started since December 2015. Globalization encourages countries to develop their own environment voices as environment has been also a global issue. Infrastructure is one of the key developments. Construction, which is an important part of infrastructure development depends heavily on the concrete industry. Ready-mix concrete is preferred material for construction because it has a good quality, ready to use, and cheaper. The thirty mega construction projects which have to be completed in 2019 will push ready-mix concrete industries to grow fast. This grow will undoubtedly have an impact on the environment.The purpose of this study is to observe the environmental impact of ready-mix concrete industry. This will cover an observation of life cycle perspective implementation, identification of the main potential environmental impacts and risks, identification of control and influence, and identification of the potential improvement opportunities. This study will hopefully contribute to the improvement of environmental awareness and stimulate more exhaustive research or study about environmental impact in Ready-mix concrete industry in the future. The method of this qualitative study is site observation and indirect investigation which included 15 plants at multiple locations. This study also addressed idea(s) around the understanding of life cycle perspective approach as described in the newest version of ISO 14001-2015 and ISO 14004-2016.
A review of joint diseases
Authors: Sadhale Varsha vishwanath 
Published: April 2021

In India more than 10 million cases of joint diseases are reported every year and amongst these joint diseases prevalence of osteoarthritis is high where patient ends up doing joint replacement. But this has various after effects and financial burden on society. Many patients approach Ayurved for the treatment of joint diseases. A proper differential diagnosis is important to provide accurate treatment so as to prevent further deterioration of patients .The approach of Ayurved and Modern science differs from diagnostic as well treatment point of view. The detail structural elucidation of each joint is not mentioned in classical texts. And hence main emphasis is given on wholesome status of bodily condition rather than specific joint. In Ayurved the patient is diagnosed only on the basis of symptomatology. whereas in modern science diagnostic tools such as MRI, X ray ,USG, CT scan ,blood investigation are used to know the exact condition of the joint . A critical review of joint diseases is done here in describing the differential diagnosis according Ayurved and Modern science.