bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Patellofemoral Morphology and Alignment With the Radiographic Severity of Patellofemoral Osteoarthritis
Authors: Dai Yike , Yin Heyong , Xu Chongyang , Zhang Hongrui , Guo Ai , Diao Naicheng 
Published: June 2021

Abstract
Background: Risk factors for the progression of patellofemoral osteoarthritis (PFOA) are poorly understood. This research aims to evaluate the association between patellofemoral joint (PFJ) morphology and alignment with the radiographic severity of PFOA.Methods: A retrospective analysis of CT scan and lateral radiograph data were acquired in patients with PFOA. The radiographic severity of PFOA and tibiofemoral OA (TFOA), lateral and medial trochlear inclination angle, sulcus angle and the Wiberg classification of patella morphology, the congruence angle, patellar tilt angle, and lateral patellar angles, and tibial tubercle trochlear groove distance (TT-TG), patella height (i.e., Caton-Deschamps index) were assessed using CT scans and sagittal radiographs of the knee. All the PFJ morphology and alignment data were divided into quarters and the relationship between each measure and the severity of PFOA was investigated.Results: By studying 150 patients with PFOA, we found a U-shaped relationship between the Caton-Deschamps index and the severity of PFOA (P <0.001). A lower value of sulcus angle and lateral patellar angle, a higher value of congruence angle, and type III patella were associated with more severity of lateral PFOA. Compared with the highest quarter of each measure, the adjusted odds ratios (OR) of the severity of PFOA in the lowest quarter of sulcus angle, lateral patellar angle, and congruence angle, and type I patella was 8.80 (p=0.043), 16.51 (P <0.001), 0.04(P <0.001), and 0.18 (p=0.048) respectively.Conclusions: Extreme value of patella height, a higher value of lateral patellar displacement and lateral patellar tilt, lower value of sulcus angle, and type III patella were associated with more severity of PFOA.
Research on Silicone Methacrylates Containing Dental Resins With Anti-adhesion Effect Against S. Mutans and Resistance to Mucin Adsorption
Authors: Tong Hui , Liao Muzi , Huang Xiangya , Liu Fang , Mai Sui , He Jingwei 
Published: June 2021

Abstract
With the purpose of preparing anti-bacterial adhesion dental resin, two silicone methacrylates (SMAs) were synthesized. After being confirmed by the FT-IR and 1H-NMR spectra, SMAs were incorporated into commonly used Bis-GMA/TEGDMA (50/50, wt./wt.) dental resin system with a series of concentration. Physicochemical properties, anti-bacterial adhesion effect, and protein adsorption were tested. The results showed that SMAs had no influence on the double bond conversion of dental resin, and could decrease volumetric shrinkage of dental resin. Because of the increased hydrophobicity and reduced surface free energy, SMAs containing cured resin had resistance to mucin adsorption and anti-adhesion effect against S. mutans. However, flexural properties, water sorption and solubility of dental resin were impaired after introducing SMAs. Therefore, further research should be taken to improve these properties by utilizing appropriate inorganic fillers.
Environmental factors influencing the composition of phyllosphere bacterial communities in bamboo: A staple food source of giant pandas
Authors: Long Juejie , Luo Wei , Xie Jianmei , Yuan Yuan , Wang Jia , Kang Liwen , Li Yi , Zhang Zejun , Hong Mingsheng 
Published: June 2021

Abstract
Background

The giant panda has developed a series of evolutionary strategies to adapt to a bamboo diet. The abundance and diversity of the phyllosphere microbiome change dramatically depending on the season, host species, location etc., which may, in turn, affect the growth and health of host plants. However, few studies have investigated the factors that influence phyllosphere bacteria in bamboo, a staple food source of the giant panda.
Methods

Amplicon sequencing of the 16S rRNA gene of rRNA genomic loci was used to explore the abundance and diversity of phyllosphere bacteria in three bamboo species (Arundinaria spanostachya, Yushania lineolate and Fargesia ferax) over different seasons (spring vs. autumn), elevation, distance from water, etc. in Liziping National Nature Reserve (Liziping NR), China.
Results

The results show that a total of 2,562 operational taxonomic units (OTUs) were obtained from all 101 samples, which belonged to 24 phyla and 608 genera. Proteobacteria was the dominant phyla, followed by Acidobacteria and Actinobacteria. The Sobs index and Shannon index of F. ferax phyllosphere bacteria were greater than that of the other two bamboo species in both seasons. The Sobs index and Shannon index of phyllosphere bacteria in all three bamboo species in autumn were significantly higher than in spring. Season was a stronger driver of community structure of phyllosphere bacteria than host bamboo species based on the (un)weighted UniFrac distance matrix. Many bacteria phyla were negatively correlated with elevation and distance from water, but positively related to mean height of bamboo and mean base diameter of bamboo. Function prediction of PICRUSt revealed the relative abundance of transporters function was highest in all three bamboo species, followed by ABC transporters. There were nine relative abundance pathways with significant differences in the 3-level KEGG pathway. The genes related to membrane transport, signal transduction and porphyrin transport in phyllosphere bacteria of F. ferax were significantly lower than in the other two species.
Conclusions

The composition, diversity and community structure of phyllosphere bacteria in bamboo, a staple food source of giant pandas, were primarily affected by the season, species, altitude, tree layer and shrub layer. The better the growth of bamboo forests, the richer the bacterial phyla in the bamboo phyllosphere. Our study presented a deeper understanding of factors influencing the bacterial community in the bamboo phyllosphere. These findings could provide a reference for the restoration and management of giant panda habitat and food resources in this area, especially for those small isolated populations of giant pandas in Xiaoxiangling mountains.
Development of a Conceptual Framework for Linking mHealth Applications to eRecord Systems in Botswana
Authors: Ndlovu Kagiso , Mars Maurice , Scott Richard E. 
Published: June 2021

Abstract
Background: The proliferation of mHealth solutions and eRecord systems is inevitable in developing countries, and ensuring their bi-directional interoperability is essential. Interoperability has been described as the ability for two or more systems or components to exchange information and use the information that has been exchanged. Given the importance of linking mHealth solutions to eRecord systems in the developing world, a suitable interoperability framework is required to provide an agreed approach to interoperability and specify common elements. Although eHealth interoperability frameworks exist in the literature, none meet all the requirements for linking mHealth solutions to eRecord systems in developing countries. The aim of this study was to describe the design and development of a conceptual framework for linking mHealth solutions to eRecord systems in Botswana, as an exemplar.Methods: An iterative and reflective process was adopted, supported by existing literature and research including consultations with eHealth experts, and guidance from existing frameworks. These collectively identified key elements, concepts, and standards relevant and essential for framework design and development.Results: The mHealth-eRecord Interoperability Framework (mHeRIF) was developed which highlights the need for: governance and regulation of mHealth and eRecord systems, a national health information exchange, and which interoperability levels to achieve. Each of these are supported by integral themes and concepts. It also addresses the need for regular review, accreditation, and alignment of framework concepts and themes with a National eHealth Strategy Interoperability Development Process. To demonstrate the framework’s applicability, a proposed architecture for the Kgonafalo mobile telemedicine programme is presented.Conclusion: Interoperable mHealth solutions and eRecords systems have the potential to strengthen health systems. This study reports the design and development of an evidence-based mHeRIF to align with, build upon, and expand National eHealth Strategies by guiding the linking of mHealth solutions to eRecord systems in Botswana and other developing countries facing similar circumstances.
Beehives Possess Their Own Distinct Microbiomes
Authors: Santorelli Lorenzo , Wilkinson Toby , Abdulmalik Ronke , Rai Yuma , Creevey Christopher J , Huws Sharon , Gutierrez-Merino Jorge 
Published: June 2021

Abstract
Honey bees use plant material to manufacture their own food. These insect pollinators visit flowers repeatedly to collect nectar and pollen, which are shared with other hive bees to produce honey and beebread. While producing these products, beehives accumulate a tremendous amount of microbes, including bacteria that derive from plants and different parts of the honey bees’ body. In this study, we conducted 16S rDNA metataxonomic analysis on honey and beebread samples that were collected from 15 beehives in the southeast of England in order to quantify the bacteria associated with beehives. The results highlighted that honeybee products carry a significant variety of bacterial groups that comprise bee commensals, environmental bacteria and pathogens of plants and animals. Remarkably, this bacterial diversity differs amongst the beehives, suggesting a defined fingerprint that is affected, not only by the nectar and pollen gathered from local plants, but also from other environmental sources. In summary, our results show that every hive possesses their own distinct microbiome, and that honeybee products are valuable indicators of the bacteria present in the beehives and their surrounding environment.
Hyperoside Suppresses Osteoclasts Differentiation and Function through Down-Regulating TRAF6/p38 MAPK Signaling Pathway
Authors: Zhu Jun , Zhang Min , Liu Xiong-Li , Yin Zhi-Gang , Han Xiao-Xue , Wang Hui-Juan , zhou Ying 
Published: June 2021

Abstract
Background: Osteoporosis (OP) is a systemic metabolic bone disease that the bone resorption exceeds the bone formation,resulting in reduced bone mass, degeneration of the microstructure of bone tissue, and then increased bone fragility and fracture risk. Hyperoside (HP), as a natural product, can promote proliferation and differentiation of osteoblasts and presents a protective effect on ovariectomized (OVX) mice. However, the inhibitory effect of HP on osteoclasts (OCs) and the potential mechanism remains to be elucidated.Methods: In this study, RAW264.7 cells were used to generate OCs induced by RANKL, and HP was applied to the cell model. The effect of HP on OCs differentiation by TRAP-positive cell counting and TRAP activity test; Bone resorption assay and actin ring formation assay were used to verify the effect of HP on OCs function; The expression levels of osteoclast-specific genes and proteins were proved by RT-PCR and Western blotting.Results: HP significantly suppressed RANKL-induced OCs differentiation, function and the mRNAs expression levels of the osteoclast-related genes. Western Blotting results demonstrated that HP reduced the expression level of TNF receptor-associated factor 6 (TRAF6) and inhibited the p38 MAPK signing pathway by reducing the phosphorylation level of p38, which subsequently down-regulated the expression level of c-fos and NFATc1, ultimately, led to a decrease of the osteoclast-specific proteins CTSK and TRAP. In addition, TRAP-positive cell counting and TRAP activity test showed that HP could inhibit the differentiation of OCs; Bone resorption assay indicated that HP could restrain the OCs’ bone resorptive activity. Actin ring formation assay showed HP can cause the shrinkage of osteoclasts and disruption of actin ring structure.Conclusion: These results revealed that HP had an inhibitory effect on OCs by down-regulating TRAF6/p38 MAPK signaling pathway. Therefore, HP could be a promising natural compound for lytic bone diseases.
Circular RNA circLOC101928570 Suppress Systemic Lupus Erythematosus Progression Via Targeting the miR-150/c-myb Axis
Authors: Zhao Xingwang , Zhang Longlong , Wang Juan , Song Zhiqiang , Ni Bing , You Yi 
Published: June 2021

Abstract
Background: Accumulating evidence supports the implication of circRNAs in systemic lupus erythematosus (SLE). however, little is known about their the detailed mechanisms and the roles of circRNAs in the pathogenesis of SLE.Methods: Quantitative real time-PCR (qRT-PCR) was used to determine the levels of circLOC101928570 and miR-150 in peripheral blood mononuclear cells (PBMCs) of SLE. Overexpression and knockdown experiments were conducted to assess the effects of circLOC101928570. Fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), luciferase reporter assays, western blot, flow cytometry analysis and enzyme-linked immunosorbent assay (ELISA) were used to investigate the molecular mechanisms underlying the function of circLOC101928570. Results: The results showed that the level of circLOC101928570 was significantly down-regulated in SLE and correlated with systemic lupus erythematosus disease activity index (SLEDAI). Functionally, circLOC101928570 acted as a miR-150 sponge to relieve the repressive effect on its target c-myb, which modulates the activation of immune inflammatory responses. CircLOC101928570 knockdown enhanced apoptosis. Moreover, circLOC101928570 promote the transcriptional level of IL2RA through directly regulate miR-150/c-myb axis. Conclusion: Overall, our findings demonstrated that circLOC101928570 played a critical role in SLE. The down-expression of circLOC101928570 suppressed SLE progression through miR-150/c-myb/IL2RA axis. Our findings identified that circLOC101928570 serve as a potential biomarker for the diagnosis and therapy of SLE.
Randomized Trial of the Feasibility of ED-Initiated School-Based Asthma Medication Supervision (ED-SAMS)
Authors: Gerald Lynn B , Gerald Joe K , VanBuren John , Lowe Ashley , Guthrie Cecilia , Klein Eileen , Morrison Andrea , Startup Emily , Denninghoff Kurt 
Published: June 2021

Abstract
Background: While using an inhaled corticosteroid (ICS) in the weeks after an ED visit reduces repeat visits, few children receive a needed prescription. Because a prescription may not be filled or used, dispensing ICS at discharge and supervising its use at school could overcome both barriers until follow-up care is established. To assess the feasibility of such an intervention, we conducted a pilot study among elementary-age school children with persistent asthma who were discharged from the ED following an asthma exacerbation. Methods: Eligible children were randomly assigned to (a) ED-dispensing of ICS with home supervision or ED-dispensing of ICS with home and school supervision. The primary outcomes were ability to recruit and retain participants, ability to initiate school-supervised medication administration within 5 days of discharge, and participant satisfaction. Results: Despite identifying 437 potentially eligible children, only 13 (3%) were enrolled with 6 being randomized to the intervention group and 7 to the control group. Eleven (85%) randomized participants completed the 90-day interview (primary outcome) and 8 (62%) completed the 120-day interview (safety endpoint). Four (67%) intervention participants started their school regimen within 5 business days and 2 started within 6 business days.Conclusion: While our pilot study did not meet its recruitment goal, it did achieve its primary purpose of assessing feasibility before undertaking a larger, more intensive study. Several major recruitment barriers need to be mitigated before EDs can successfully partner with schools to establish supervised ICS treatment. Clinical Trial Registry: ClinicalTrials.gov, NCT03952286. Registered May 16,2019, https://clinicaltrials.gov/ct2/show/NCT03952286?term=ED+SAMS&draw=2&rank=1
Disease Severity Is an Independent Risk Factor of Mortality and Outcomes in Critically Ill Patients With Carbapenem-resistant Klebsiella Pneumoniae Bloodstream Infections: a 5-year Retrospective Analysis
Authors: Qiu Yuzhen , Xu Wen , Dai Yunqi , Tan Ruoming , Liu Jialin , Gu Feifei , Chen Erzhen , Wang Xiaoli , Qu Hongping 
Published: June 2021

Abstract
Background: Carbapenem-resistant Klebsiella pneumoniae bloodstream infections (CRKP-BSIs) are associated with high morbidity and mortality rates, especially in critically ill patients. Comprehensive mortality risk analyses and therapeutic assessment in real-world practice are beneficial to guide individual treatment.Methods: We retrospectively analyzed 87 patients with CRKP-BSIs (between July 2016 and June 2020) to identify the independent risk factors for 28-day all-cause mortality. The therapeutic efficacies of tigecycline-and polymyxin B-based therapies were analyzed.Results: The 28-day all-cause mortality and in-hospital mortality rates were 52.87% and 67.82%, respectively, arising predominantly from intra-abdominal (56.32%) and respiratory tract infections (21.84%). A multivariate analysis showed that 28-day all-cause mortality was independently associated with the patient’s APACHE II score (p = 0.002) and presence of septic shock at BSI onset (p = 0.006). All-cause mortality was not significantly different between patients receiving tigecycline- or polymyxin B-based therapy (55.81% vs. 53.85%, p = 0.873), and between subgroups mortality rates were also similar. Conclusions: Critical illness indicators (APACHE II scores and presence of septic shock at BSI onset) were independent risk factors for 28-day all-cause mortality. There was no significant difference between tigecycline- and polymyxin B-based therapy outcomes. Prompt and appropriate infection control should be implemented to prevent CRKP infections.
Gene-gene Interaction of AhR With and Within the Wnt Cascade Affects Susceptibility to Lung Cancer
Authors: Rosenberger Albert , Muttray Nils , Hung Rayjean J , Christiani David C , Caporaso Neil E , Liu Geoffrey , Bojesen Stig E , Marchand Loic Le , Albanes Demetrios , Aldrich Melinda C , Tardon Adonina , Fernández-Tardón Guillermo , Rennert Gad , Field John K , Davies Michael P.A. , Liloglou Triantafillos , Kiemeney Lambertus A , Lazarus Philip , Wendel Bernadette , Haugen Aage , Zienolddiny Shanbeh , Lam Stephen , Schabath Matthew B , Andrew Angeline S , Duell Eric J , Arnold Susanne M , Goodman Gary E , Chen Chu , Doherty Jennifer A , Taylor Fiona , Cox Angela , Woll Penella J , Risch Angela , Muley Thomas R , Johansson Mikael , Brennan Paul , Landi Maria Teresa , Shete Sanjay S , Amos Christopher I , Bickeböller Heike 
Published: June 2021

Abstract
Introduction: Aberrant Wnt signalling, regulating cell development and stemness, is observed in many cancer entities. Aryl hydrocarbon receptor (AhR) mediates tumorigenesis of environmental pollutants. Complex interaction patterns of genes assigned to AhR/Wnt-signalling were recently associated to lung cancer susceptibility. Aim: To assess the association and predictive ability of AhR/Wnt-genes with lung cancer in cases and controls of European descent. Methods: Odds ratios (OR) were estimated for genomic variants assigned to the genes DKK2, DKK3, DKK4, FRZB, SFRP4 and Axin2 and other lung cancer-related genes. Logistic regression models with variable selection were trained, validated and tested to predict lung cancer. Further, decision trees were created to investigate variant x variant interaction. All analyses were performed for overall lung cancer and for subgroups. Results: No association with overall lung cancer was observed, but within the subgroups of ever smokers (e.g. maker rs2722278 SFRP4; OR=1.20; 95%-CI: 1.13-1.27; p=5.6 10-10) and never smokers. Although predictability is poor, AhR/Wnt-variants are unexpected overrepresented in optimized prediction scores for overall lung cancer and for small cell lung cancer. Remarkable, the score for never-smokers contained solely two AhR/Wnt-variants. The optimal decision tree for never smokers consists of 7 AhR/Wnt-variants and only two lung cancer variants, no assigned to any CHRN gene. Conclusions: The role of variants belonging to Wnt/AhR-pathways in lung cancer susceptibility may be underrated in main-effects association analysis. Complex interaction patterns in individuals of European descent have moderate predictive capacity for lung cancer or subgroups thereof, especially in never smokers.
Circ_0005232 Promotes the Progression of Oral Squamous Cell Carcinoma by Sponging miR-1299 to Regulate CDK6
Authors: Zhang Xue , Guo Guang-Yu , Wang Zhen-Hua , Zhang Zhong-Ti 
Published: June 2021

Abstract
Objectives: CircRNA may play essential roles and act as biomarkers in tumor development due to their special stable structure. However, the mechanism by which circRNAs affect OSCC progression is still unclear. Methods: qRT-PCR was performed to detect circ_0005232 expression level in oral squamous cell carcinoma (OSCC) tissues and cell lines. Colony formation assays, cell migration and invasion assays, and wound healing assays were performed to verify the effects of overexpression or knockdown of circ_0005232 on the biological function of OSCC cell lines. Western blot was performed to determine the effects of circ_0005232 on epithelial-to-mesenchymal transition (EMT) and expression of MMP2 and MMP9 in OSCC cell lines. Dual luciferase reporter assays, rescue assays, RNA immunoprecipitation assays, and EDU incorporation assays were performed to explore interactions among circ_0005232, miR-1299, and CDK6. Results: qRT-PCR results confirmed that circ_0005232 was expressed significantly higher in OSCC tissue and cell lines. Functional experiments indicated that overexpression of circ_0005232 promoted OSCC cell lines proliferation，migration and invasion ability, while inhibition of circ_0005232 caused opposite results. MiR-1299 knockdown could rescue the changes in cell function caused by circ_0005232 knockdown. The dual luciferase reporter assay verified that circ_0005232 could bind with miR-1299 to affect the proliferation，migration and invasion ability of OSCC cell lines. RNA immunoprecipitation assays indicated that circ_0005232 could increase CDK6 expression by sponging miR-1299.Conclusions: Our results demonstrate that circ_0005232 exerts its tumor-promoting effects by sponging miR-1299 which then affects function of CDK6. Therefore, circ_0005232 may represent a novel potential prognostic biomarker and therapeutic target in OSCC.