bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predominance of Genetically Diverse Esbl Escherichia Coli Identified in Resistance Mapping of Largest Fresh Cum Brackish Water of Vembanad Lake, India
Authors: Vaiyapuri Murugadas , Sebastian Anna SherinPulithara , George Iris , Variem Sandhya Soolamkandath , Vasudevan Radhakrishnan Nair , George Joshy Chalil , Badireddy Madhusudana Rao , Sivam Visnuvinayagam , Peeralil Shaheer , Sanjeev Devi , Thandapani Muthulakshmi , Moses Sheela Albert , Nagarajarao Ravishankar Chandragiri , Mothadaka Mukteswar Prasad 
Published: April 2021

Abstract
Antimicrobial resistance (AMR) burden in Escherichia coli along the 90 km stretch of Vembanad Lake, Kerala, India was assessed. Seventy-seven percent of water samples drawn from 35 different stations of the Lake harbored E. coli. Antibiotic susceptibility test performed on 116 E. coli isolates revealed 81% were resistant to ≥ one antibiotic with 39 AMR profiles, 30% multidrug resistant, 32% extended spectrum b lactamase (ESBL) producers as per CLSI.The probability of isolating cefotaxime resistant E. coli was the highest 0.7 (P ≤ 0.05) in the Lake. Genetically diverse ESBL types blaTEM-116,blaCTX-M -152, blaCTX-M -27,blaCTX-M -55, blaCTX-M-205, and blaSHV-27 were identified. Molecular typing (ERIC PCR, MLST and PBRT) confirmed the diversity among E. coli between and within the stations. ST11439 and Single and Double Loci Variants of ST443, ST4533 were identified in Multi locus sequence typing (MLST) analysis. Inc plasmids (B/O, F, W, I1, FIIA, HI1, P-1a, K/B and N) identified in the Lake evidences the transmission potential. Low multiple antibiotic resistance index (average < 0.2) indicating lower risk to the human population albeit, an emerging concern of ESBL resistance in the Lake. The occurrence of genetically variant ESBL E. coli in Vembanad Lake signals health hazards and necessitates pragmatizing strategic control measures.
Does Beneficiaries’ Satisfaction Enhance Their Trust in State Grid for Solar PV Adoption? – An Evidence From PAPs in Rural China
Authors: Ding Liping , Shi Yin , Dai Qiyao , Zhang Zumeng , Li Jiaxin , Zhou Ling 
Published: April 2021

Abstract
Background: Photovoltaic Poverty Alleviation Projects (PPAPs) have been implemented in Chinese rural areas since 2014. As a new energy policy, PPAPs have played an important role in alleviating rural poverty. However, the adoption of solar PV faces multiple barriers from the perspective of beneficiaries. Therefore, this study basically aims to discuss and analyze factors affecting beneficiaries’ satisfaction and their trust in State Grid, promoting the adoption of solar PV. Methods: Based on the integrated American Customer Satisfaction Index (ACSI) and Unified Theory of Acception and Use of Technology (UTAUT) model, this study uses the Structural Equation Model (SEM) to reveal how the beneficiaries’ satisfaction enhance their trust in State Grid. The data were obtained from a survey of 928 PPAPs’ beneficiaries by stratified and random sampling in Chinese rural areas. Results: The results confirm that environmental perception in this study positively impacts on beneficiaries’ satisfaction. And perceived quality also has a positive effect on beneficiaries’ satisfaction and trust in State Grid, however, social influence has a negative impact on beneficiaries’ satisfaction; behavior expectation can directly promote beneficiaries’ satisfaction, while indirectly propel their trust in State Grid. Conclusions: This study constructs an integrated customer satisfaction model from the perspective of beneficiaries and proposes relevant measures to promote the adoption of solar PV that can be applied to poverty reduction of other developing countries worldwide.
Wet Deposition of Total Dissolved Nitrogen in Indo-Gangetic Plain (India)
Authors: Mishra Manisha , Kulshrestha Umesh Chandra 
Published: April 2021

Abstract
Very limited information on the magnitude and environmental impacts of both inorganic as well as organic forms of Nitrogen (N) wet deposition is available in India. Molar concentrations of inorganic (NH4+ and NO3−) and organic N in rainwater were monitored at three different land-use sites in Indo-Gangetic Plain (IGP) during the monsoon period (June-September) of 2017. It has been observed that dissolved organic N (DON) contributed significantly to the total dissolved N (TDN) ranging from 5–60%. Dissolved inorganic N (DIN = NH4+ + NO3−) concentration was recorded as high as 221.0 µmol L− 1 at urban site to as low as 65.9 µmol L− 1 at the rural site. A similar pattern was also observed for DON. NH4+ contribution to TDN had the order: urban megacity (65%) > urban (70%) > rural (75%). Agriculture and animal husbandry are the primary sources of NH4+ emissions in the rural site. However, NO3− has shown a contrasting trend at these sites (25%, 15% and 8%, respectively). Wet deposition fluxes of atmospheric TDN was observed to be higher at urban sites. This can attributed to a variety of local sources such as vehicular emission, microbial emissions, biomass burning, human excreta due to higher population density, and transportation from surrounding areas, as observed from concentration weighted trajectories (CWT) model and cluster analysis. Upwind region of IGP has experienced major influence of air mass transported from agriculturally rich northwest part of India. However, both the downwind sites have experienced by-and-large the influence of south-westerly air-masses originated over the Arabian Sea. Study has found that the DON contributes significantly to TDN and need to be included for budget assessment in South Asia.
Covid-19 Vaccination May Cause FDG Uptake Beyond Axillary Area
Authors: Fleury Vincent , Maucherat Bruno , Rusu Daniela , Dumont Frédéric , Rousseau Caroline 
Published: April 2021

Abstract
Background The vaccination immune response may induced false-positive 18F-FDG PET/CT uptake.Case presentationAn extended supraclavicular lymph nodal activation after Coronavirus Disease 2019 (Covid-19) vaccination revealed on 18F-FDG PET/CT mimics a Virchow nodule in a patient with medical history of well-differentiated appendicular adenocarcinoma. Conclusion This case highlights a nodal activation beyond axillary area and the importance of documenting vaccination history at the time of scanning to avoid false-positive results.
Limonin Modulated Immune and Inflammatory Responses to Suppress Colorectal Adenocarcinoma in Mice Model
Authors: Ishak Nur Iliyani Mohd , MOHAMED SUHAILA , Madzuki Iffah Nadhira , Mustapha Noordin Mohamed , Esa Norhaizan Mohd 
Published: April 2021

Abstract
Purpose. Inflammation and compromised immune responses often increases colorectal cancer (CRC) risk. The immune-modulating effects of limonin on carcinogen/inflammation-induced colorectal cancer (CRC) were studied in mice. Methods: Male Balb/c mice were randomly assorted into three groups (n=6): healthy control, non-treated CRC-induced (azoxymethane/dextran-sulfate-sodium AOM/DSS) control, and CRC-induced+50 mg limonin /kg body-weight. The CRC development were monitored via macroscopic, histopathological, ELISA and mRNA expression analysis. Results: Limonin downregulated inflammation (TNF-α, tumor necrosis factor-α), enhanced the adaptive immune responses (CD8, CD4, and CD19), and upregulated antioxidant defense (Nrf2, SOD2) mRNA expressions. Limonin reduced serum malondialdehyde (MDA, lipid peroxidation biomarker), prostaglandin-E2 and histopathology inflammation scores, while increasing reduced-glutathione (GSH) in the CRC-induced mice. Limonin significantly (p<0.05) increased T cells (CD4 and CD8) and B cells (CD19) in spleen tissues. The CD335 (natural killer cells) were increased in the CRC-induced mice and limonin treatment restored it to normal levels suggesting reinstatement to normal colon conditions.Conclusion: Limonin apparently mitigated CRC development, by ameliorating adaptive immune responses (CD8, CD4, and CD19), reducing inflammation (serum prostaglandin-E2; TNF-α, innate immune responses), oxidative stress and enhancing the endogenous anti-oxidation defense reactions (GSH) in the CRC-induced mice.
The impact of the timely birth-dose vaccine on the global elimination of hepatitis B
Authors: de Villiers Margaret , Nayagam Shevanthi , Hallett Timothy 
Published: April 2021

Abstract
Scaling-up timely hepatitis B birth-dose vaccination to 90% of new-borns in 110 low- and middle-income countries by 2030 could prevent 770,000 (600,000–1,060,000) deaths in the 2020 to 2030 birth cohorts compared to status quo, with the greatest benefits in Africa. Maintaining this could lead to elimination by 2032 in the Americas, but not before 2059 in Africa. Drops in coverage due to disruptions in 2020 may lead to 17,000 additional deaths, mostly in South-East Asia and the Western Pacific. Delays in planned scale-up could lead to an additional 630,000 deaths globally in the 2020 to 2030 birth cohorts.
Surfactant-Free Cellulose Filaments Stabilized Oil in Water Emulsions
Authors: Varamesh Amir , Prathapan Ragesh , Telmadarreie Ali , Li Jia , Gourlay Keith , Minhas Gurminder , Lu Qingye , Bryant Steven L. , Hu Jinguang 
Published: April 2021

Abstract
There has been significant interest over recent years in the production and application of sustainable and green materials. Among these, nanocellulose has incurred great interest because of its exceptional properties and wide range of potential applications, including in Pickering emulsions. However, the production cost of these cellulosic materials has limited their application. In this study, the capability of a new type of cheaper cellulosic material, cellulose filaments (CFs), in formulating stable oil in water Pickering emulsions was investigated and compared with three conventional nanocelluloses, namely cellulose nanocrystals (CNCs), cellulose nanofibrils (CNFs) and TEMPO-oxidized CNFs (TEMPO-CNFs). Results showed that CFs can provide stable surfactant-free emulsions over wide ranges of salt concentration (0 – 500 mM) and pH (2 – 10), as indicated by the near constant oil droplet size and dewatering index of the emulsions. This is due to the ability of CFs to strongly adsorb to the oil and water interface, as evidenced by visualizing labeled CFs with engineered carbohydrate-binding module (CBM2a) conjugated with green fluorescent protein (CBM2a-eGFP) under fluorescent microscopy. Compared to the emulsions stabilized by other types of nanocelluloses, the CFs-stabilized emulsion demonstrated a larger average droplet size and comparable (with CNFs) or better (than CNCs and TEMPO-CNFs) stability, which is partially attributed to the higher viscosity of continuous phase in the presence of CFs. The results of this study demonstrate the use of CFs as a novel and cheaper cellulosic material for stabilizing emulsions, which opens the door to a range of markets from the food industry to engineering applications.
Controlled Dispersion and Setting of Cellulose Nanofibril (CNF) - Carboxymethyl Cellulose (CMC) Pastes
Authors: Azrak Sami Miguel El Awad , Gohl Jared , Moon Robert , Schueneman Gregory , Davis Chelsea , Youngblood Jeffrey 
Published: April 2021

Abstract
This work investigated the redispersion and setting behavior of highly loaded (~18 wt.% solids in water) pastes of cellulose nanofibrils (CNFs) with carboxymethyl cellulose (CMC). A single-screw extruder was used to continuously process CNF+CMC pastes into cord. The adsorption of CMC onto the CNF fibrils was assessed through zeta potential and titration which revealed a surface charge change of ~61 % from -36.8 mV and 0.094 mmol/g COOH for pure CNF to -58.1 mV and 0.166 mmol/g COOH for CNF+CMC with a CMC degree of substitution of 0.9. Dried CNF with adsorbed CMC was found to be fully redispersible in water and re-extruded back into a cord without any difficulties. On the other hand, chemical treatment with hydrochloric acid, a carbodiimide crosslinker, or two wet strength enhancers (polyamide epichlorohydrin and polyamine epichlorohydrin) completely suppressed the dispersibility previously observed for dried-untreated CNF+CMC. Turbidity was used to quantify the level of redispersion or setting achieved by the untreated and chemically treated CNF+CMC in both water and a strong alkaline solution (0.1 M NaOH). Depending on the chemical treatment used, FTIR analysis revealed the presence of ester, N-acyl urea, and anhydride absorption bands which were attributed to newly formed linkages between CNF fibrils, possibly explaining the suppressed redispersion behavior. Water uptake of the differently treated and dried CNF+CMC materials agreed with both turbidity and FTIR results.
Bibliometric Analysis of Phosphogypsum Research from 1990 to 2020 Based on Literatures and Patents
Authors: Cao Yunmeng , Cui Yue , Yu Xiaokun , Li Tong , Chang I-shin , WU Jing 
Published: April 2021

Abstract
The demand together with the urgency of phosphogypsum (PG) treatment, will pose big challenges for many countries. This research aims to explore the research progress of PG, including basic status, cooperation situation, research fields and development trends, based on Web of Science database through bibliometric analysis of publications (articles and patents) from 1990 to 2020. The results show that the academic research of PG originated earlier, but the number of patents grew faster. China has an absolute advantage in the numbers and plays a significant role in international cooperation. The knowledge structure of PG is mainly concentrated in the fields of natural radioactivity, cement paste backfilling, soil, crystal morphology, and synthetic gas. Academic hotspots focus on the microstructure of chemical processes and various environmental impacts, and the patents hot technologies is the production of refractory materials, ceramics, surface materials, cement mortar and composite materials. The academic frontiers of PG will be the methods of recovering rare earth elements from PG, the conditions of ion solidification/stabilization in PG, the impact of reaction conditions on product quality, and the reaction mechanism at the micro-level. The frontiers of patents will focus on the improvement of manufacturing equipment, new wall materials and chemical modified polymer materials. In terms of the number of articles and patents in the future, architectural research has the maximum space for improvement. This paper conducts an in-depth analysis of PG and provides information on the technological development prospects and opportunities, which is helpful for researchers engaged in PG management.
Percolation transitions in compressed SiO2 glasses
Authors: Hasmy Anwar , Ispas Simona , Hehlen Bernard 
Published: April 2021

Abstract
Amorphous-amorphous transformations under pressure are generally explained by changes in the local structure from low to higher fold coordinated polyhedra [1-4].
However, as the notion of scale invariance at the critical thresholds has not been addressed, it is still unclear whether these transformations could be associated to true phase transitions.
Here we report ab initio based calculations of compressed silica (SiO2) glasses showing that the structural changes from low- to high-density amorphous structures occur through a sequence of percolation transitions.
When the pressure is increased up to 82 GPa, a series of long range ('infinite') percolating clusters built up by corner- or edge-shared tetrahedra, pentahedra, and eventually octahedra, emerge at some critical pressures and replace the previous phase of lower fold coordinated polyhedra and lower connectivity.
This mechanism provides a natural explanation for the well-known mechanical anomaly around 3 GPa as well as for the structural irreversibility beyond 10 GPa, among others.
Some of the amorphous structures that have been discovered mimic those of coesite IV and V crystals reported recently [5,6], highlighting the major role of SiO5 pentahedra-based polyamorphs in the densification process of vitreous silica.
Our observations demonstrate that the percolation theory provides a robust framework to understand the nature and the pathway of the amorphous-amorphous transformations, and open a new avenue to predict unraveled amorphous solid phases and related liquids [7,8].
Catalytic Oxidation of Toluene Over LaNixB1-xO3 (B=Co, Cu) Perovskite Catalysts
Authors: Qi Songya , Zhang Wanqi , Li Xiang , Wang Qing , Zhu Zerui , Zhou Ting , Wang Gaoxiang , Xie Aijuan , Luo Shiping 
Published: April 2021

Abstract
A series of LaNixB1-xO3 (B=Co, Cu) perovskite catalysts were prepared by sol-gel method and employed in toluene degradation. The catalysts were characterized by XRD, UV-Vis, SEM and XPS to investigate crystal structure, morphology and composition of the catalyst. The effect of different calcination temperatures and time on the performance of perovskite catalysts for toluene degradation were investigated. And the CO2 selectivity was also carried out on as-prepared catalysts. The results showed that a small amount of Co-doping in B site of LaNiO3 could significantly improve its toluene degradation, while the substitution of Ni with Cu could not effectively enhance its activity. When the Ni/Co molar ratio in LaNixCo1-xO3 perovskite was 3:1, LaNi0.75Co0.25O3 catalyst exhibited the best activity, and it showed relatively short time and low temperature for toluene conversion of 100%. Therefore, LaNi0.75Co0.25O3 catalyst has a broad prospect in the degradation of volatile organic pollutants.
Alleviation of Salinity and Metal Stress Using Plant Growth-Promoting Rhizobacteria Isolated From Semiarid Moroccan Copper-Mine Soils
Authors: Madline Atika , Benidire Leila , Boularbah Ali 
Published: April 2021

Abstract
Phytoremediation is an eco-friendly method for rehabilitation of mine tailing using plants and their associated rhizosphere microorganisms. Some heavy metal and salt-tolerant plant growth promoting rhizobacteria (PGPR) could be beneficial in alleviating soil salinity and heavy metal stress. The aim of this work is to select PGPR that could be used in phytoremediation process. Twenty-nine rhizobacteria were examined for their ability to grow at increasing concentrations of NaCl and high concentrations of Zn, Pb, Cu and Cd. The results showed that seventeen rhizobacteria displayed high salinity and metal tolerance (up to 100g L− 1 NaCl, up to 5 mM Cd, 9 mM Pb, 10 mM Zn, and Cu up to 6 mM). This work showed also that salinity and metallic stress has affected bacterial growth and metabolism by increasing intracellular proline, soluble sugars, free amino-acids and exopolysaccharides production. Moreover, almost all tested bacteria maintained their PGP traits under 10 % of NaCl and multi-metal stress. Four strains exhibiting the best PGP activities namely Mesorhizobium tamadayense, Enterobacter xiangfangensis, Pseudomonas azotifigens and Streptomyces Caelestis were selected for root elongation bioassay. The consortium of these rhizobacteria improves significantly the root elongation of Peganum harmala and Lactuca sativa under metallic and salt stress. Thus, the rhizobacteria with beneficial traits as well as tolerance to abiotic stress could be useful to stimulate plants establishment under different environmental stresses.
Genomic a Nalysis and Molecular Characteristics in Carbapenem R Esistant Klebsiella Pneumoniae Strains
Authors: Zhao Su-ying , Qiu Xingyi , Tan Yiting , Xu Ming , Xu Xiaotong , Ge Yulai , Cao Huiling , Li Min , Lin Yuxin , Zhang Zhan , Li Lei , Wu Qian 
Published: April 2021

Abstract
Background

Klebislla pneumoniae is an important bacterium and responsible for both infections acquired in hospital and community because of its multidrug resistance and the virulence. The aim of this research was to investigate clonal lineages, antibiotic resistance profiles and virulence factors of the hospital isolated Carbapenem Resistant strains.
Methods

Whole-genome sequencing of one strain was performed using the Illumina Hiseq and PacBio RS systems. Genomic information was comprehensively analyzed by various bioinformatics approaches. Molecular typing was based on multi locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis. Antimicrobial susceptibility testing, drug resistance and virulence genes were determined.
Results

K. pneumoniae KPX comprised one circular chromosome and four circular plasmids. This strain harbors a variety of antimicrobial resistance and virulence determinants. The closest relative of K. pneumoniae KPX was another ST11 clinical isolate recovered Sichuan. Fifty isolates were phenotypically confirmed extended-spectrum beta-lactamases ESBLs producers. MLST analysis revealed 97% sequence type 11. Fifty Carbapenem Resistant isolates mainly belong to three clones according to the PFGE DNA patterns. PFGE patterns has more variety than ST profiles. In addition, KPC-2 (98.0%), SHV-11 (98.0%), TEM-1 (76.0%), CTX-M (76.0%), Oqxb1(66%), qnrS (70%), Int1 (42.0%), sul1 (82.0%), sul2 (96.0%), iutA (88%), iucABCD(10%), and rmpA2 (100%) genes were presented in multiple drug resistant strains.
Conclusion

The dataset presented in this study provided the genomic and epidemiological analysis of Carbapenem Resistant K. pneumoniae in hospital settings. Antimicrobial-resistance profiles suggested the presence of significant selective antibiotic pressure. Appropriate surveillance is essential to development of effective control strategies in the prevention of nosocomial infection.
Theoretical Analysis of Safety Thickness of the Water-Resistant Rock Mass of Karst Tunnel Face Taking Into Account Seepage Effect
Authors: GUO Jiaqi , Wu Wenlong , Liu Xiliang , Huang Xin , Zhu Zhengguo 
Published: April 2021

Abstract
This paper took into account the adverse influence of the karst water seepage effect on the water-resistant rock mass. Based on the upper-bound theorem of limit analysis and the Hoek-Brown failure criterion, through a series of formula derivation, the expression of critical safety thickness of water-resistant rock mass of karst tunnel face was finally obtained. The paper carried out a feasibility analysis, an analysis of influencing factors and a comparative analysis with previous related research achievements of this method. The results showed that: (1) With the decrease of surrounding rock grade, the safety thickness of water-resistant rock mass gradually increased, and the safety thickness of surrounding rock at all grades remained within a reasonable range. (2) The safety thickness decreased as the compressive strength, the tensile strength and parameter A increased, and it increased as the karst water pressure, the tunnel excavation height, and parameter B increased. (3) The change trend of the safety thickness with the influencing factors was completely consistent under the two conditions of considering and without the seepage effect, and the safety thickness with considering the seepage force was greater than that without considering the seepage force. Taking the Yunwushan tunnel of Yiwan railway as an example, the critical safety thickness of the water-resistant rock mass was calculated and the calculated value was in good coincidence with the safety thickness adopted in the actual project. The research results are of great significance to prevent the occurrence of high pressure filling karst geological disasters such as water inrush in tunnels.