bluuebunny/crossref_metadata_2025_split huggingface dataset:
Topological modular forms and the absence of a heterotic global anomaly
Authors: Tachikawa Yuji 
Published: May 2021

Abstract
Spacetime theories obtained from perturbative string theory constructions are automatically free of perturbative anomalies, but it is not settled whether they are always free of global anomalies. Here we discuss a possible $\mathbb{Z}_{24}$-valued pure gravitational anomaly of heterotic compactifications down to two spacetime dimensions, and point out that it can be shown to vanish using the theory of topological modular forms, assuming the validity of the Stolz–Teichner conjecture.
Best Laid Plans: How the Middle Class Make Residential Decisions Post-Disaster
Authors: Rhodes Anna , Besbris Max 
Published: May 2021

Abstract
Middle-class households typically search for housing with an eye to long-term residence and are able to maintain a high level of stability, but what happens when these households are forced to make mobility decisions after exposure to a disaster and subsequent residential displacement? Through longitudinal interviews with 59 households in a middle-class suburb of Houston that flooded during Hurricane Harvey, we found that residential mobility decisions—whether to stay and rebuild or move—were guided by households’ durable plans about the future. The majority of households decided to remain and rebuild their homes, despite the ability to move and pressure from friends and family to relocate to less vulnerable places with similar amenities. The households that stayed had long-term plans to remain in their homes before the flood, while the small number who decided to move generally had well-defined plans to do so in the near future before the storm hit. Our findings reveal the role of plans in the residential decision-making of middle-class households and have implications for understanding post-disaster immobility at a time when middle-class households are increasingly exposed to environmental disasters.
Effects of prescribed fire timing on grazing performance of yearling beef cattle, forage biomass accumulation, and plant community characteristics on native tallgrass prairie in the Kansas Flint Hills
Authors: Duncan Zachary M , Tajchman Alan J , Ramirez Micke P , Lemmon Jack , Hollenbeck William R , Blasi Dale A , Fick Walter H , Olson K C 
Published: May 2021

Abstract
Recent research demonstrated that mid- or late-summer prescribed fires can be employed to manage sericea lespedeza (Lespedeza cuneata) infestations in the Kansas Flint Hills. The effects of prescribed fire applied during the growing season (i.e., August to October) on grazing performance of yearling cattle have not been evaluated. Native pastures (n = 18; 22 ± 4.0 ha) were grouped by watershed and assigned randomly to one of three prescribed-fire treatments: spring (7 April ± 2.1 d), summer (21 August ± 5.7 d), or autumn (2 October ± 9.9 d). Yearling beef cattle were grazed from May to August at a targeted stocking density of 280 kg live-weight/ha following prescribed-fire application. Forage biomass accumulations, soil cover, plant species composition, and root carbohydrate concentrations in four native plant species were evaluated. Total body weight (BW) gains and average daily gain were greater (P = 0.01) for cattle that grazed the spring and summer prescribed-fire treatments compared with those that grazed the autumn prescribed-fire treatment. As a result, final BW were greater (P = 0.04) in the spring and summer treatments than the autumn treatment. Conversely, forage biomass accumulations did not differ (P = 0.91) between fire regimes. Proportions of bare soil were greater (P &lt; 0.01) in the spring treatment compared with the summer and autumn treatments, whereas proportions of litter on the soil surface were greater (P &lt; 0.01) in summer- and autumn-burned pastures compared with spring-burned pastures. Total basal cover of graminoids and forbs did not differ (P ≤ 0.15) between prescribed fire treatments. Likewise, total basal cover of C3 or C4 perennial grasses did not differ (P ≥ 0.23) between prescribed-fire treatments. No treatment differences (P = 0.24) in root starch or root water-soluble carbohydrate concentrations in big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), Indiangrass (Sorghastrum nutans), or purple prairieclover (Dalea purpurea) were detected. These data were interpreted to suggest that summer or autumn prescribed fire can be applied without reducing forage biomass accumulations, root carbohydrate concentrations in key native plant species, or considerably altering native plant populations compared with conventional spring-season prescribed fire; however, summer prescribed fire could be favored over spring or autumn prescribed fire both to maintain stocker cattle growth performance and to achieve control over sericea lespedeza.
Use of Generalizability Theory for Exploring Reliability of and Sources of Variance in Assessment of Technical Skills: A Systematic Review and Meta-Analysis
Authors: Andersen Steven Arild Wuyts , Nayahangan Leizl Joy , Park Yoon Soo , Konge Lars 
Published: May 2021

Purpose
Competency-based education relies on the validity and reliability of assessment scores. Generalizability (G) theory is well suited to explore the reliability of assessment tools in medical education but has only been applied to a limited extent. This study aimed to systematically review the literature using G-theory to explore the reliability of structured assessment of medical and surgical technical skills and to assess the relative contributions of different factors to variance.

Method
In June 2020, 11 databases, including PubMed, were searched from inception through May 31, 2020. Eligible studies included the use of G-theory to explore reliability in the context of assessment of medical and surgical technical skills. Descriptive information on study, assessment context, assessment protocol, participants being assessed, and G-analyses was extracted. Data were used to map G-theory and explore variance components analyses. A meta-analysis was conducted to synthesize the extracted data on the sources of variance and reliability.

Results
Forty-four studies were included; of these, 39 had sufficient data for meta-analysis. The total pool included 35,284 unique assessments of 31,496 unique performances of 4,154 participants. Person variance had a pooled effect of 44.2% (95% confidence interval [CI], 36.8%–51.5%). Only assessment tool type (Objective Structured Assessment of Technical Skills-type vs task-based checklist-type) had a significant effect on person variance. The pooled reliability (G-coefficient) was 0.65 (95% CI, .59–.70). Most studies included decision studies (39, 88.6%) and generally seemed to have higher ratios of performances to assessors to achieve a sufficiently reliable assessment.

Conclusions
G-theory is increasingly being used to examine reliability of technical skills assessment in medical education, but more rigor in reporting is warranted. Contextual factors can potentially affect variance components and thereby reliability estimates and should be considered, especially in high-stakes assessment. Reliability analysis should be a best practice when developing assessment of technical skills.
Discrepancies Between Preceptor and Resident Performance Assessment: Using an Electronic Formative Assessment Tool to Improve Residents’ Self-Assessment Skills
Authors: Schultz Karen , McGregor Tara , Pincock Rob , Nichols Kathleen , Jain Seema , Pariag Joel 
Published: May 2021

Problem
Accurate self-assessment is a critical skill for residents to develop to become safe, adaptive clinicians upon graduation. Physicians need to be able to identify and fill in knowledge and skill gaps to deal with the rapid expansion of medical knowledge and unpredicted novel emerging medical issues. Residency training to date has not consistently focused on building these overarching skills, nor have the burgeoning assessment data that competency-based medical education (CBME) affords been used beyond their initial intent to inform summative assessment decisions. Both are important missed opportunities.

Approach
The Queen’s University Family Medicine Program adopted CBME in 2010. In 2011, it added the capacity for residents to electronically self-assess their daily performance, with preceptors reviewing and modifying as needed before submitting. In 2018, it designed software to report discordance between residents’ self-assessment and preceptors’ assessment of performance.

Outcomes
From 2011–2019, 56,585 field notes were submitted, 11,429 by residents, with 28% of those (3,200/11,429) showing discordance between residents’ and preceptors’ performance assessments. When discordant, residents assessed their performance as less competent (undercalled) than their preceptor did 73% of the time (2,336/3,200 field notes). For the 864 field notes (27% of 3,200 discordant notes) where residents rated their performance higher than their preceptor did (overcalled, for 162/1,120 [14%] residents), 6 residents overcalled performance to a dangerous extent (2 or 3 levels of supervision higher than what their supervisors assessed them at) and 26 repeatedly (greater than 5 times) overcalled their level of performance by 1 supervisory level.

Next Steps
Inaccurate self-assessment (both overcalling and undercalling performance) has negative consequences. Awareness is a first step in addressing this. Discrepancy reports will be used during regular academic reviews with residents to discuss the nature, degree, and frequency of discrepancies, with the intent of fostering improved self-assessment of performance.
Efficacy and safety of secukinumab in Chinese patients with moderate-to-severe plaque psoriasis: a real-life cohort study
Authors: Zhao Yan , Cai Lin , Liu Xiao-Yang , Zhang Heng , Zhang Jian-Zhong 
Published: May 2021

Abstract

Background:
There have been few real-life dose-comparing studies on the efficacy and safety of secukinumab in Chinese patients with plaque psoriasis. We conducted a real-life cohort study to investigate the efficacy and safety of secukinumab 150 and 300 mg in Chinese patients with moderate-to-severe plaque psoriasis.

Methods:
A total of 106 patients with moderate-to-severe plaque psoriasis were included in this study. Patients received either secukinumab 150 mg or secukinumab 300 mg according to patients’ weights and severity of psoriasis. The treatment continued for at least 24 weeks. The efficacy was evaluated by improvement in the psoriasis area and severity index (PASI) scores. The safety was also analyzed.

Results:
Fifty-nine patients (55.7%) were treated with secukinumab 300 mg and 47 patients (44.3%) were treated with secukinumab 150 mg. After 12-week treatment, PASI75/90/100 responses were achieved in 100%, 97.8%, and 95.7% of patients, respectively, in secukinumab 150 mg group, and the efficacy was maintained to week 24. In secukinumab 300 mg group, PASI75/90/100 responses were achieved in 93.2%, 81.4%, and 76.3% of patients, respectively, at week 12. In this group, PASI75/90/100 responses reached 91.5%, 86.4%, and 79.9%, respectively, at week 24. Biologic-experienced patients had lower responses than biologic-naïve patients. Secukinumab 150 and 300 mg were well tolerated. Five patients discontinued treatment due to poor response, adverse event, or economic reasons.

Conclusions:
This real-life study demonstrated that high PASI 90 and PASI 100 responses were achieved in Chinese psoriasis patients receiving secukinumab 150 or 300 mg. Biologic-naïve was associated with better clinical efficacy.
Interpretation of temporal and spatial trends of SARS-CoV-2 RNA in San Francisco Bay Area wastewater
Authors: Greenwald Hannah D. , Kennedy Lauren C. , Hinkle Adrian , Whitney Oscar N. , Fan Vinson B. , Crits-Christoph Alexander , Harris-Lovett Sasha , Flamholz Avi I. , Al-Shayeb Basem , Liao Lauren D. , Beyers Matt , Brown Daniel , Chakrabarti Alicia R. , Dow Jason , Frost Dan , Koekemoer Mark , Lynch Chris , Sarkar Payal , White Eileen , Kantor Rose , Nelson Kara L. 
Published: May 2021

ABSTRACTWastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be integrated with COVID-19 case data to inform timely pandemic response. However, more research is needed to apply and develop systematic methods to interpret the true SARS-CoV-2 signal from noise introduced in wastewater samples (e.g., from sewer conditions, sampling and extraction methods, etc.). In this study, raw wastewater was collected weekly from five sewersheds and one residential facility, and wastewater SARS-CoV-2 concentrations were compared to geocoded COVID-19 clinical testing data. SARS-CoV-2 was reliably detected (95% positivity) in frozen wastewater samples when reported daily new COVID-19 cases were 2.4 or more per 100,000 people. To adjust for variation in sample fecal content, crAssphage, pepper mild mottle virus, Bacteroides ribosomal RNA (rRNA), and human 18S rRNA were evaluated as normalization biomarkers, and crAssphage displayed the least spatial and temporal variability. Both unnormalized SARS-CoV-2 RNA signal and signal normalized to crAssphage had positive and significant correlation with clinical testing data (Kendall’s Tau-b (τ)=0.43 and 0.38, respectively). Locational dependencies and the date associated with testing data impacted the lead time of wastewater for clinical trends, and no lead time was observed when the sample collection date (versus the result date) was used for both wastewater and clinical testing data. This study supports that trends in wastewater surveillance data reflect trends in COVID-19 disease occurrence and presents approaches that could be applied to make wastewater signal more interpretable and comparable across studies.
Selection of Human Single Domain Antibodies (sdAb) Against Thymidine Kinase 1 and Their Incorporation Into sdAb-Fc Antibody Constructs For Potential Use In Cancer Therapy
Authors: Velazquez Edwin J. , Cress Jordan D. , Humpherys Tyler B. , Mortimer Toni O. , Bellini David M. , Skidmore Jonathan R , Smith Kathryn R. , Robison Richard A. , Weber Scott K. , O’Neill Kim L. 
Published: May 2021

AbstractThymidine Kinase 1 (TK1) is primarily known as a cancer biomarker with good prognostic capabilities for liquid and solid malignancies. However, recent studies targeting TK1 at protein and mRNA levels have shown that TK1 may be useful as a tumor target. In order to examine the use of TK1 as a tumor target, it is necessary to develop therapeutics specific for TK1. Single domain antibodies (sdAbs), represent an exciting approach for the development of immunotherapeutics due to their cost-effective production and higher tumor penetration than conventional antibodies. In this study, we isolated sdAb fragments specific to human TK1 from a human sdAb library. A total of 400 sdAbs were screened through 5 rounds of selection by monoclonal phage ELISA. The most sensitive sdAb fragments were selected as candidates for preclinical testing. The sdAb fragments showed specificity for human TK1 in phage ELISA, Western blot analysis and had a limit of detection of 3.9 ng/ml for 4-H-TK1_A1 and 1.9 ng/ml for 4-H-TK1_D1. The antibody fragments were successfully expressed and used for detection of membrane associated TK1 (mTK1) through flow cytometry on cancer cells [lung (∼95%), colon (∼87%), breast (∼53%)] and healthy human mono nuclear cells (MNC). The most sensitive antibody fragments, 4-H-TK1_A1 and 4-H-TK1_D1 were fused to an engineered IgG1 Fc fragment. When added to cancer cells expressing mTK1 co-cultured with human MNC, the anti-TK1-sdAb-IgG1_A1 and D1 were able to elicit a significant antibody-dependent cell-mediated cytotoxicity (ADCC) response by human MNCs against lung cancer cells compared to isotype controls (P<0.0267 and P<0.0265, respectively). To our knowledge this is the first time that the isolation and evaluation of human anti TK1 single domain antibodies using phage display technology has been reported. The antibody fragments isolated here may represent a valuable resource for the detection and the targeting of TK1 in tumor cells.
GLOBAL LAND-USE AND LAND-COVER DATA: HISTORICAL, CURRENT AND FUTURE SCENARIOS
Authors: Vale Mariana M. , Lima-Ribeiro Matheus S. , Rocha Tainá C. 
Published: May 2021

AbstractLand-use land-cover (LULC) data are important predictors of species occurrence and biodiversity threat. Although there are LULC datasets available under current conditions, there is a lack of such data under historical and future climatic conditions. This hinders, for example, projecting niche and distribution models under global change scenarios at different time scenarios. The Land Use Harmonization Project (LUH2) is a global terrestrial dataset at 0.25° spatial resolution that provides LULC data from 850 to 2300 for 12 LULC state classes. The dataset, however, is compressed in a file format (NetCDF) that is incompatible for many analyses and requires layer extractions and transformations that are intractable for most researchers. Here we selected and transformed the LUH2 in a standard GIS format data to make it more user-friendly. We provide LULC for every year from 850 to 2100, and from 2015 on, the LULC dataset is provided under two Shared Socioeconomic Pathways (SSP2-4.5 and SSP5-8.5). We provide two types of files for each year: separate files with continuous values for each of the 12 LULC state classes, and a single categorical file with all state classes combined. To create the categorical layer, we assigned the state with the highest value in a given pixel among the 12 continuous data. LUH2 predicts a pronounced decrease in primary forest, particularly noticeable in the Amazon, the Brazilian Atlantic Forest, the Congo Basin and the boreal forests, an equally pronounced increase in secondary forest and non-forest lands, and in croplands in the Brazilian Atlantic Forest and sub-Saharan Africa. The final dataset provides LULC data for 1251 years that will be of interest for macroecology, ecological niche modeling, global change analysis, and other applications in ecology and conservation.
iCodon: ideal codon design for customized gene expression
Authors: Medina-Muñoz Santiago Gerardo , Diez Michay , Castellano Luciana Andrea , da Silva Pescador Gabriel , Wu Qiushuang , Bazzini Ariel Alejandro 
Published: May 2021

AbstractMessenger RNA (mRNA) stability substantially impacts steady-state gene expression levels in a cell. mRNA stability, in turn, is strongly affected by codon composition in a translation-dependent manner across species, through a mechanism termed codon optimality. We have developed iCodon (www.iCodon.org), an algorithm for customizing mRNA expression through the introduction of synonymous codon substitutions into the coding sequence. iCodon is optimized for four vertebrate transcriptomes: mouse, human, frog, and fish. Users can predict the mRNA stability of any coding sequence based on its codon composition and subsequently generate more stable (optimized) or unstable (deoptimized) variants encoding for the same protein. Further, we show that codon optimality predictions correlate with expression levels using fluorescent reporters and endogenous genes in human cells and zebrafish embryos. Therefore, iCodon will benefit basic biological research, as well as a wide range of applications for biotechnology and biomedicine.
Unilateral Resection of Both Cortical Visual Pathways Alters Action but not Perception
Authors: Ahmad Zoha , Behrmann Marlene , Patterson Christina , Freud Erez 
Published: May 2021

AbstractThe human cortical visual system consists of two major pathways, a ventral pathway that subserves perception and a dorsal pathway that subserves visuomotor control. These pathways follow dissociable developmental trajectories, and, accordingly, might be differentially susceptible to neurodevelopmental disorders or injuries. Previous studies have found that children with cortical resections of the ventral visual pathway retain largely normal visuoperceptual abilities. Whether visually guided actions, supported by computations carried out by the dorsal pathway, follow a similar pattern remains unknown. To address this question, we examined visuoperceptual and visuomotor behaviors in a pediatric patient, TC, who underwent a cortical resection that included portions of the left ventral and dorsal pathways. We collected data when TC used her right and left hands to perceptually estimate the width blocks that varied in width and length, and, separately, to grasp the same blocks. TC’s perceptual estimation performance was comparable to that of controls, independent of the hand used. In contrast, relative to controls, she showed reduced visuomotor sensitivity to object shape and this was more evident when she grasped the objects with her contralesional right hand. These results provide evidence for a striking difference in the reorganization profiles of the two visual pathways. This difference supports the notion that the two pathways exhibit differential susceptibility to neurodevelopmental disorders.
Phase separation in fluids with many interacting components
Authors: Shrinivas Krishna , Brenner Michael P. 
Published: May 2021

AbstractFluids in natural systems, like the cytoplasm of a cell, often contain thousands of molecular species that are organized into multiple coexisting phases that enable diverse and specific functions. How interactions between numerous molecular species encode for various emergent phases is not well understood. Here we leverage approaches from random matrix theory and statistical physics to describe the emergent phase behavior of fluid mixtures with many species whose interactions are drawn randomly from an underlying distribution. Through numerical simulation and stability analyses, we show that these mixtures exhibit staged phase separation kinetics and are characterized by multiple coexisting phases at equilibrium with distinct compositions. Random-matrix theory predicts the number of existing phases at equilibrium, validated by simulations with diverse component numbers and interaction parameters. Surprisingly, this model predicts an upper bound on the number of phases, derived from dynamical considerations, that is much lower than the limit from the Gibbs phase rule, which is obtained from equilibrium thermodynamic constraints. Using a biophysically motivated model of pairwise interactions between components, we design ensembles that encode either linear or non-monotonic scaling relationships between number of components and co-existing phases, which we validate through simulation and theory. Finally, inspired by parallels in biological systems, we show that including nonequilibrium turnover of components through chemical reactions can tunably modulate the number of coexisting phases at steady-state without changing overall fluid composition. Together, our study provides a model framework that describes the emergent dynamical and steady-state phase behavior of liquid-like mixtures with many interacting constituents.