bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Plasmodium falciparum infection and fitness of genetically modified Anopheles gambiae aimed at mosquito population replacement
Authors: Tapanelli Sofia , Inghilterra Maria Grazia , Cai Julia , Philpott James , Capriotti Paolo , Windbichler Nikolai , Christophides George K. 
Published: November 2021

AbstractGenetically modified (GM) mosquitoes expressing anti-plasmodial effectors propagating through wild mosquito populations by means of gene drive is a promising tool to support current malaria control strategies. The process of generating GM mosquitoes involves genetic transformation of mosquitoes from a laboratory colony and, often, interbreeding with other GM lines to cross in auxiliary traits. These mosquito colonies and GM lines thus often have different genetic backgrounds and GM lines are invariably highly inbred, which in conjunction with their independent rearing in the laboratory may translate to differences in their susceptibility to malaria parasite infection and life history traits. Here, we show that laboratory Anopheles gambiae colonies and GM lines expressing Cas9 and Cre recombinase vary greatly in their susceptibility to Plasmodium falciparum NF54 infection. Therefore, the choice of mosquitoes to be used as a reference when conducting infection or life history trait assays requires careful consideration. To address these issues, we established an experimental pipeline involving genetic crosses and genotyping of mosquitoes reared in shared containers throughout their lifecycle. We used this protocol to examine whether GM lines expressing the antimicrobial peptide (AMP) Scorpine in the mosquito midgut interfere with parasite infection and mosquito survival. We demonstrate that Scorpine expression in the Peritrophin 1 (Aper1) genomic locus reduces both P. falciparum sporozoite prevalence and mosquito lifespan; both these phenotypes are likely to be associated with the disturbance of the midgut microbiota homeostasis. These data lead us to conclude that the Aper1-Sco GM line could be used in proof-of-concept experiments aimed at mosquito population replacement, although the impact of its reduced fitness on the spread of the transgene through wild populations requires further investigation.
Immunoglobulin-producing AT2-like cells secrete IgA into the extracellular matrix in pulmonary fibrosis
Authors: Chen Mengling , Wang Jing , Yuan Mengqin , Long Min , Wang Sha , Luo Wei , Huang Jie , Cheng Yusi , Zhang Wei , Jiang Wei , Chao Jie 
Published: November 2021

AbstractPulmonary fibrosis is an interstitial lung disease that can be caused by various factors. Here, we first observed extensive IgA deposition in the extracellular matrix (ECM) of the lungs of mice with pulmonary fibrosis induced by silica inhalation. Consistent with this phenomenon, spatial transcriptomic sequencing of fresh mouse lung tissues from control mice and model mice showed that Igha transcripts were highly expressed in the lesion area. Single-cell RNA sequencing (scRNA-seq) and reconstruction of B cell receptor (BCR) sequences revealed a new cluster of cells with a shared BCR and high expression of genes related to immunoglobulin IgA production. Surprisingly, these clonal cells had more characteristics of AT2 (alveolar epithelial cell type 2) cells than B cells; thus, these cells were named AT2-like cells. Therefore, we propose that secretion of IgA into the ECM by AT2-like cells is an important process that occurs during lung fibrosis.
Mouse Organ Specific Proteins and Functions
Authors: Sun B. , Lorang C. , Qin S. , Zhang Y. , Liu K. , Li G. , Sun Z. , Francke A. , Utleg A. G. , Flores P. , Hu Z. , Wang K. , Moritz R. , Hood L. 
Published: November 2021

ABSTRACTOrgan specific proteins (OSPs) possess great medical potentials both in clinics and in biomedical research. Applications of them — such as alanine transaminase, aspartate transaminase, and troponins — in clinics have raised certain concerns of their organ specificity. The dynamics and diversity of protein expression in heterogeneous human population are well known, yet their effects on OSPs are less addressed. Here we use mouse as a model and implemented a scheme of breadth study to examine the pan-organ proteome for potential variations of organ specificity in different genetic backgrounds. Using reasonable resources, we generated pan-organ proteomes of four in-bred mouse strains. The results revealed a large diversity that is more profound among OSPs than the overall proteomes. We defined a robustness score to quantify such variation and derived three sets of OSPs with different stringencies. In the meantime, we found that the enriched biological functions of OSPs are also organ specific that are sensitive and useful to assess the quality of OSPs. We hope our breadth study can open doors to explore the molecular diversity and dynamics of organ specificity at the protein level.
Universal Calcium fluctuations inHydramorphogenesis
Authors: Agam Oded , Braun Erez 
Published: November 2021

ABSTRACTUnderstanding how the collective physical processes drive robust morphological transitions in animal development requires the characterization of the relevant fields underlying morphogenesis. Calcium (Ca2+) is known to be such a field. Here we show that the Ca2+spatial fluctuations, in whole-bodyHydraregeneration, exhibit universal properties captured by a field-theoretic model describing fluctuations in a tilted double-well potential. We utilize an external electric field andHeptanol, a drugblocking gap junctions, as two separate controls affecting the Ca2+activity and pausing the regeneration process in a reversible way. Subjecting theHydratissue to an electric field increases the calcium activity and its spatial correlations, while applyingHeptanolinhibits the activity and weakens the spatial correlations. The statistical characteristics of the Ca2+spatial fluctuations – i.e., the coefficient of variation and the skewness - exhibit universal shape distributions across tissue samples and conditions, demonstrating the existence of global constraints over this field. Our analysis shows that theHydra’s tissue resides near the onset of bistability; the local Ca2+activity in different regions fluctuates between low and high excited states. The controls modulate the dynamics near that onset, preserving the universal characteristics of the Ca2+fluctuations and, by that, maintaining the tissue’s ability to regenerate.
SARS-CoV-2 infection induces cross-reactive autoantibodies against angiotensin II
Authors: Briquez Priscilla S. , Rouhani Sherin J. , Yu Jovian , Pyzer Athalia R. , Trujillo Jonathan , Dugan Haley L. , Stamper Christopher T. , Changrob Siriruk , Sperling Anne I. , Wilson Patrick C. , Gajewski Thomas F. , Hubbell Jeffrey A. , Swartz Melody A. 
Published: November 2021

ABSTRACTPatients infected with the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can experience life-threatening respiratory distress, blood pressure dysregulation and thrombosis. This is thought to be associated with an impaired activity of angiotensin-converting enzyme-2 (ACE-2), which is the main entry receptor of SARS-CoV-2 and which also tightly regulates blood pressure by converting the vasoconstrictive peptide angiotensin II (AngII) to a vasopressor peptide. Here, we show that a significant proportion of hospitalized COVID-19 patients developed autoantibodies against AngII, whose presence correlates with lower blood oxygenation, blood pressure dysregulation, and overall higher disease severity. Anti-AngII antibodies can develop upon specific immune reaction to the SARS-CoV-2 proteins Spike or RBD, to which they can cross-bind, suggesting some epitope mimicry between AngII and Spike/RBD. These results provide important insights on how an immune reaction against SARS-CoV-2 can impair blood pressure regulation.
Effect of wall covering materials on the perception of cafe environments
Authors: Coşgun Büşra , Yıldırım Kemal , Hidayetoglu Mehmet Lutfi 
Published: November 2021

PurposeThis study aims to determine the effects of wall covering materials (wood, concrete and metal) used indoors on participants’ perceptual evaluations. The differences among participants’ perceptual evaluations regarding indoor physical environmental factors by occupation and gender were examined.Design/methodology/approachCafes were selected as research environments. Virtual experimental spaces using three different wall covering materials were modelled and participants’ assessment of the physical environmental factors of these virtual spaces was measured through a detailed questionnaire.FindingsCafes using light-coloured wall covering materials were perceived more favourably than cafes using dark-coloured wall covering materials, and cafes with light-coloured wooden wall coverings were considered as a warmer material than cafes using concrete and metal. Participants who received design education (architect, interior architect) perceived physical environmental factors of cafes more negatively than those who did not receive design education (lawyer, economist, accountant, etc.). Male participants evaluated the physical environmental factors of cafes more positively than female participants for all adjective pairs. Except for two adjective pairs, no significant difference was found among the evaluations according to genders for the other adjective pairs.Originality/valueThis study revealed new results about customers’ choices of wall covering materials and offered designers new alternatives for materials that can be used in the design of cafes.
The G20, climate change and COVID-19: critical juncture or critical wound?
Authors: Johnstone Injy 
Published: November 2021

PurposeThe Group of 20 (G20) is tasked with responding to economic shocks in the global financial system, with COVID-19 having proved to be the most significant shock since the G20's inception. COVID-19 also represents the first economic crisis accompanied by a concerted attempt to “build back better”, principally through a climate-compatible recovery. In 2021, there is little clarity as to the G20's response to this challenge, primarily due to considerable divergence in the green stimulus practices of its member states. The paper aims to investigate whether the G20, climate change and COVID-19 are critical juncture or critical wound.Design/methodology/approachHistorical institutionalism (HI) suggests that one can explain an institution's future response by reference to its developmental pathway to date. This contribution adopts its concept of “critical junctures” to shed light on the G20's possible institutional response to COVID-19. The contribution undertakes a comparative analysis of the global financial crisis (GFC) and COVID-19 as possible critical junctures for the G20.FindingsIn doing so, the work demonstrates that the G20 “building back better” from COVID-19 requires a shift away from its institutional orthodoxy to a much larger degree than its response to the GFC. Accordingly, whilst both the GFC and COVID-19 may be considered critical junctures for the G20, only COVID-19 has the potential to be a “critical wound” that leads to institutional redundancy.Research limitations/implicationsThrough interrogating this further, this exposition prospectively outlines two possible futures the G20 faces as a consequence of COVID-19: reform or redundancy. In this way, it offers an ex ante perspective on policy-reform options for the G20's ongoing response to COVID-19.Practical implicationsWhichever choice the G20 makes in its response to COVID-19 has profound consequences for global governance in an increasingly unpredictable world.Originality/valueHerein lies the importance of an exploratory assessment of COVID-19 as a critical juncture or a critical wound for the G20.
Aerodynamic simulation of a high-pressure compressor stage using the lattice Boltzmann method
Authors: de Laborderie Jerome , Babin Cedric , Fontaneto Fabrizio 
Published: November 2021

Purpose
The present paper aims at evaluating the lattice Boltzmann method (LBM) on a high-subsonic high-pressure compressor stage at nominal regime.

Design/methodology/approach
The studied configuration corresponds to the H25 compressor operated in a closed-loop test rig at the von Karman Institute. Several operating points are simulated with LBM for two grids of successive refinements. A detailed analysis is performed on the time-averaged flow predicted by LBM, using a comparison with experimental and existing RANS data.

Findings
The finest grid is found to correctly predict the mean flow across the machine, as well as the influence of the rotor tip gap size. Going beyond time-averaged data, some flow analysis is performed to show the relevance of such a high-fidelity method applied to a compressor configuration. In particular, vortical structures and their evolution with the operating points are clearly highlighted. Spectral analyses finally hint at a proper prediction of tonal and broadband contents by LBM.

Originality/value
The application of LBM to high-speed turbomachinery flows is very recent. This paper validates one of the first LBM simulations of a high-subsonic high-pressure compressor stage.
Examining the use of fsQCA in B2B marketing research: benefits, current state and agenda for future research
Authors: Gligor David Marius , Golgeci Ismail , Rego Carla , Russo Ivan , Bozkurt Sıddık , Pohlen Terrance , Hiatt Brian , Garg Vipul 
Published: November 2021

Purpose
The purpose of this paper is to build on recent efforts occurring within business-to-business (B2B) marketing research to advance methodological developments. As phenomena within B2B relationships have become increasingly complex, marketing scholars have begun to point out the limitations associated with correlation-based methodological approaches and highlight the need for new developments in this area. One such development is the fuzzy set qualitative comparative analysis (fsQCA).

Design/methodology/approach
This study uses a literature review to examine the use of fsQCA in B2B marketing research.

Findings
First, the current manuscript presents the benefits that the application of fsQCA can offer to market researchers investigating B2B phenomena. Second, the paper presents the current state of fsQCA use within B2B marketing. Third, it suggests possible marketing B2B research topics that can be explored using fsQCA.

Originality/value
The study highlights the benefits of fsQCA, presents the current state of fsQCA use within B2B marketing and offers a rich future research agenda for B2B marketing scholars. This agenda can also help spur additional method developments in the discipline.
The asymmetric and threshold impact of external debt on economic growth: new evidence from Egypt
Authors: Sharaf Mesbah Fathy 
Published: November 2021

PurposeWithin a multivariate framework, this study examines the asymmetric and threshold impact of external debt on economic growth in Egypt during the period 1980–2019.Design/methodology/approachThe paper uses a nonlinear autoregressive distributed lag (NARDL) bounds testing approach to cointegration and a vector error-correction model to estimate the short- and long-run parameters of equilibrium dynamics. A multiple structural breaks model is estimated to test nonlinearity in the relationship between external debt and economic growth.FindingsResults of the NARDL model show a robust statistically significant negative long-run impact on economic growth stemming from both positive and negative external-debt-induced shocks. In terms of magnitude, on the one hand, the impact of external-debt-induced negative shocks exceeds that of the positive. In the short and long run, on the other hand, the growth impact of external debt in Egypt is symmetric. There is also support for the nonlinearity hypothesis in which a negative impact on growth of external debt obtains once the threshold level of external debt-to-GDP ratio equals or exceeds 96.7%.Practical implicationsIdentifying the threshold level after which external debt becomes harmful to economic growth would help inform policymakers in Egypt about maximum external debt levels that can be sustained without impairing economic growth.Originality/valueThe current study makes a substantial contribution to the extant literature on the debt-growth tradeoffs. It breaks ground by being the first tract that examines, using a NARDL model, asymmetry and nonlinearity of debt-growth tradeoffs in Egypt.
Clinical guidelines and clinical autonomy: exploring the missing link
Authors: Andri Maria 
Published: November 2021

PurposeThis paper aims at understanding how clinical guidelines' use in the labour process relates to clinical autonomy, that is, the self-control medical professionals exercise over medical practice.Design/methodology/approachDrawing on a qualitative case study research strategy, this paper explores how medical professionals use clinical guidelines in the labour process in one public general hospital of the Greek National Health System. Supplemented by an extensive study of documents, semi-structured interviews were conducted with 33 doctors of several specialties.FindingsThe analysis shows (1) how clinical autonomy, as a self-control structure, mediates the use of clinical guidelines as a knowledge tool in the labour process, and (2) how employing clinical guidelines as a means towards coordinating medical work, but also towards regulating and standardising medical practice, is exercising pressure on the individualistic character of clinical autonomy.Originality/valueAdvancing the analytic value of workplace control structures, this paper contributes novel theoretical understanding of emerging tendencies characterising medical work organisation and clinical autonomy, and explains how medical professionals' non-adherence to clinical practice guidelines (CPGs) relates to CPGs' role as a resource to medical practice. Finally, this research proposes a more critical approach to health policy towards addressing the challenges associated with centrally introducing clinical guidelines in healthcare organisations.
Member surveys on employee ownership and the COVID-19 pandemic
Authors: Rodgers Loren 
Published: November 2021

PurposeTo gather data about how employee-owned companies performed during the COVID-19 pandemic and whether employee ownership was a competitive advantage.Design/methodology/approachSurveys of members of the National Center for Employee Ownership.FindingsEmployee-owned companies were more likely to have positive than negative outcomes and many attribute part of their success to their employee ownership structure.Research limitations/implicationsData was from NCEO member companies and not a representative sample of employee-owned companies.Practical implicationsCompanies should consider employee ownership as a strategy; employee-owned companies should consider employee engagement efforts.Originality/valueThis includes the first data gathered specifically on employee-owned companies during the pandemic.
Reinterpreting a signature pedagogy for entrepreneurship education
Authors: Ramsgaard Michael Breum , Blenker Per 
Published: November 2021

PurposeThe importance of contextualizing theory development in entrepreneurship education has recently been raised. Nevertheless, efforts often lead to rather decontextualized concepts and generic theories that are unable to bring together the complexities of applying entrepreneurship education to particular institutional logics and local pedagogical understandings. Based on a narrow and selective literature review, this study aims to identify and reconstruct how entrepreneurship education can adjust to the disciplines in which it unfolds. To contribute to transcending this dilemma, this article raises the following question: How can entrepreneurship education be understood in a differentiated manner and contextually reconstructed to the many disciplines and professions in which it unfolds?Design/methodology/approachThe study follows the general idea of an integrative literature review, meaning that a few references, in particular Jones' work on a signature pedagogy for entrepreneurship education, led to a deeper search of the older background literature from Shulman on the idea of a signature pedagogy for professions.FindingsThe authors identify three existing notions (MK-0, MK-1 and MK-2) of signature pedagogy within entrepreneurship education and propose a fourth notion that combines the established understandings into a signature pedagogy, MK-3, in which entrepreneurship education should be adjusted to the disciplines in which it unfolds, by integrating and balancing general, disciplinary/professional and entrepreneurial purposes of education.Originality/valueAccepting that context matters to entrepreneurship education creates a need for understanding the contextual influence on pedagogies. The paper contributes by establishing a theory-based framework that can help educators formulate and balance general, professional and entrepreneurial purposes of education, depending on the particular context of their educational activities. Furthermore, the paper is a call to action for additional scholarship that identifies avenues for future research.
Innovating resilience
Authors: Robertson James Andrew 
Published: November 2021

Purpose:
This paper aims to review the latest management developments across the globe and pinpoint practical implications from cutting-edge research and case studies.

Design:
This briefing is prepared by an independent writer who adds their own impartial comments and places the articles in context.

Findings:
Organizational areas which deal with innovation are often the first to see costs cut when an economic crisis emerges. Yet this can actually have a dramatic negative effect on recovery after a crisis, compared to cutting costs elsewhere.

Originality:
The briefing saves busy executives, strategists and researchers hours of reading time by selecting only the very best, most pertinent information and presenting it in a condensed and easy-to-digest format.
Celebrity capitalism
Authors: Robertson James Andrew 
Published: November 2021

Purpose:
This paper aims to review the latest management developments across the globe and pinpoint practical implications from cutting-edge research and case studies.

Design:
This briefing is prepared by an independent writer who adds their own impartial comments and places the articles in context.

Findings:
Celebrity CEOs are a new type of celebrity able to profoundly impact their own companies and brands just through their personal actions, in ways previous CEOs have not been able to do.

Originality:
The briefing saves busy executives, strategists and researchers hours of reading time by selecting only the very best, most pertinent information and presenting it in a condensed and easy-to-digest format.