bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the response of tropical cyclone structure to the assimilation of synthetic wind profiles
Authors: Bucci Lisa R. , Majumdar Sharanya J. , Atlas Robert , Emmitt G. David , Greco Steve 
Published: February 2021

AbstractThis study examines how varying wind profile coverages in the tropical cyclone (TC) core, near-environment and broader synoptic environment affect the structure and evolution of a simulated Atlantic hurricane through data assimilation. Three sets of observing system simulation experiments (OSSEs) are examined in this paper. The first experiment establishes a benchmark for the case study specific to the forecast system used by assimilating idealized profiles throughout the parent domain. The second presents how TC analyses and forecasts respond to varying the coverage of swaths produced by polar-orbiting satellites of idealized wind profiles. The final experiment assesses the role of TC inner-core observations by systematically removing them radially from the center. All observations are simulated from a high-resolution regional “Nature Run” of a hurricane and the tropical atmosphere, assimilated an Ensemble Square-Root Kalman Filter and the Hurricane Weather and Research Forecast (HWRF) regional model. Results compare observation impact to the analyses, domain-wide and TC centric error statistics, and TC structural differences among the experiments. The study concludes that the most accurate TC representation is a result of the assimilation of collocated and uniform thermodynamic and kinematics observations. Intensity forecasts are improved with increased inner core wind observations, even if the observations are only available once daily. Domain-wide root-mean-square errors are significantly reduced when the TC is observed during a period of structural change, like rapid intensification. The experiments suggest the importance of wind observations and the role of inner-core surveillance when analyzing and forecasting realistic TC structure.
Preoperative detection of pleural adhesions using pocket-sized ultrasound
Authors: Sano Atsushi , Hiranuma Ayako , Nagashima Makoto 
Published: February 2021

Background Although preoperative detection of pleural adhesions is important in thoracic surgery, it is not widely performed. We report the availability of a pocket-sized ultrasound device for the preoperative detection of pleural adhesions. Methods Between September 2019 and September 2020, pleural adhesions were assessed preoperatively using a pocket-sized ultrasound device in 62 patients who underwent thoracic surgery. Evaluations were performed using the Vscan Dual Probe on the wards or just before surgery in the operating theater. We used a linear probe to scan the chest wall where the incision was scheduled, and evaluated the sliding sign. We compared ultrasound results with intraoperative findings. Results Of the 62 patients, the sliding sign was observed in 58 patients, 56 of whom demonstrated no pleural adhesions intraoperatively. The sensitivity was 96.6%. Four patients were negative for the sliding sign; of these, three had pleural adhesions and one did not. The specificity was 75.0%. Among all 62 patients, the diagnostic accuracy of ultrasound for pleural adhesions was 95.2%. False negatives were caused by loose adhesions. False positives were caused by the absence of vertical lines on ultrasound. Accuracy was not influenced by the timing of the test. Conclusions A pocket-sized ultrasound device was useful for the preoperative detection of pleural adhesions in thoracic surgery.
Radiomics analysis enables fatal outcome prediction for hospitalized patients with coronavirus disease 2019 (COVID-19)
Authors: Ke Zan , Li Liang , Wang Li , Liu Huan , Lu Xuefang , Zeng Feifei , Zha Yunfei 
Published: February 2021

Background In December 2019, a rare respiratory disease named coronavirus disease 2019 (COVID-19) broke out, leading to great concern around the world. Purpose To develop and validate a radiomics nomogram for predicting the fatal outcome of COVID-19 pneumonia. Material and Methods The present study consisted of a training dataset (n = 66) and a validation dataset (n = 30) with COVID-19 from January 2020 to March 2020. A radiomics signature was generated using the least absolute shrinkage and selection operator (LASSO) Cox regression model. A radiomics score (Rad-score) was developed from the training cohort. The radiomics model, clinical model, and integrated model were built to assess the association between radiomics signature/clinical characteristics and the mortality of COVID-19 cases. The radiomics signature combined with the Rad-score and the independent clinical factors and radiomics nomogram were constructed. Results Seven stable radiomics features associated with the mortality of COVID-19 were finally selected. A radiomics nomogram was based on a combined model consisting of the radiomics signature and the clinical risk factors indicating optimal predictive performance for the fatal outcome of patients with COVID-19 with a C-index of 0.912 (95% confidence interval [CI] 0.867–0.957) in the training dataset and 0.907 (95% CI 0.849–0.966) in the validation dataset. The calibration curves indicated optimal consistency between the prediction and the observation in both training and validation cohorts. Conclusion The CT-based radiomics nomogram indicated favorable predictive efficacy for the overall survival risk of patients with COVID-19, which could help clinicians intensively follow up high-risk patients and make timely diagnoses.
Living versus deceased kidney transplantation: Comparison of complications
Authors: Fakhr Yasseri Ali Mohammad , Namdari Farshad , Gooran Shahram , Ahmadi Ayat , Dehghani Sanaz , Asadi Mahboobeh , Mehrsay Abdolrasol , Pourmand Gholamreza , Dialameh Hossein 
Published: February 2021

Introduction: It is assumed that the outcome of kidney transplantation from living donors is more favorable than deceased donors. However, over the years there has been an overall improvement in transplant survival from both living and deceased donors. In this article we have evaluated and compared the most recent results in living and deceased donor kidney transplantations. Patients and Methods: Four hundred and forty six patients underwent kidney transplantation in our center from September 2009 to March 2014. The patients divided in two groups living (group A) and deceased (group B) donor transplantation groups. The patients were followed until September 2016. Acute rejection, graft survival, delayed graft function, renal artery thrombosis, graft nephrectomy, ureterocutaneous fistula, postoperative hypertension, mortality, hospital stay, hyperlipidemia, post transplantation diabetes and lymphocele rate measured and compared in two groups. Results: Most variables were not different between the two groups except lymphocele and delayed graft function. Lymphocele was more prevalent in group A (13.8% vs 3.1%, p-value = 0.02) and delayed graft function results were more desirable in living donor transplantation group (group B). Conclusion: Although, delayed graft function was less common in transplantation from living donors, short, and long term graft survival was not significantly different in this study.
Mindfulness-Based Stress Reduction and Hope in Women Participating in Alternative Sentencing Programs
Authors: McIntosh Heather Chancellor , Kezbers Krista , Nichols Rachel , Hellman Chan 
Published: February 2021

Purpose: Alternative sentencing programs for women with nonviolent drug offenses may provide opportunities to change the life trajectories of women and their families. The primary purpose of this study was to assess whether a mindfulness-based stress reduction (MBSR) intervention could improve levels of hope following treatment. A second objective was to assess whether MBSR intervention could influence perceived stress, self-compassion, and mindfulness. Methods: Participants attended a 6-week mindfulness class, with outcomes measured using pretest–posttest survey comparison. Results: Paired sample t tests showed improvements in hope, stress, self-compassion, and mindfulness following treatment. Discussion: Community-based intervention models, such as MBSR therapy in alternative sentencing programs, may alleviate negative psychological impacts experienced by female offenders. These results align with previous research that suggests participants may see improved ability to regulate emotions following MBSR therapy. This research indicates that MBSR therapy, for women in alternative sentencing programs, may promote hope as a learned psychological skill.
The Impact of Body Weight and Renal Function on the Risk of Bleeding With Direct Oral Anticoagulants in Atrial Fibrillation
Authors: Whittemore Hannah , Posen Andrew K. , Hellenbart Erika L. , Groo Vicki , Wenzler Eric , Tilton Jessica J. 
Published: February 2021

Background: Atrial fibrillation (AF) increases the risk of stroke and direct oral anticoagulants (DOACs) are first-line agents for prevention. Gaps in the literature cause reluctance in prescribing DOACs for patients with renal dysfunction and/or extremes in body weight. Objective: To evaluate the impact body weight and renal function have on major and clinically relevant nonmajor (CRNM) bleeding events and ischemic strokes in AF patients receiving a DOAC. Methods: This retrospective cohort study included adults with nonvalvular atrial fibrillation (NVAF) or atrial flutter (AFL) receiving a DOAC ≥12 months. The primary outcome was a composite of major and CRNM bleeding events. Secondary outcomes included ischemic stroke and risk factors for bleeding events. Results: Of the 233 patients analyzed, 25 patients experienced a bleeding event. Patients who bled weighed 10 kg less ( P = 0.043) than those who did not and had a higher HASBLED score ( P = 0.003). Multivariate logistic regression identified weight ( P = 0.048), serum creatinine (SCr; P = 0.027), and HASBLED score ( P = 0.024) as the significant predictors for experiencing a bleed. Three patients experienced a stroke. Conclusion and Relevance: This study demonstrates an association between higher baseline SCr, elevated HASBLED score, and lower weight, with an increased risk of bleeding in patients with NVAF or AFL receiving a DOAC. These findings add to prescribing considerations when initiating DOACs. Closer monitoring is advised for patients with significant renal dysfunction and/or low body weight, even with renal dose adjustments.
Efficacy of plasmapheresis as treatment for bilateral hyperviscosity syndrome related retinopathy in multiple myeloma
Authors: Merz Tommaso , Marchesoni Ivan , Caminiti Giulia , Romanelli Federica 
Published: February 2021

Purpose: To report a case of good clinical response to plasmapheresis as therapy for a bilateral hyperviscosity syndrome related retinopathy in a young patient with undiagnosed multiple myeloma (MM). Methods: Case report. Results: A 48-year-old caucasian man, previously diagnosed with monoclonal gammopathy of undetermined significance (MGUS), presented for medical attention for back ache and vision decrease, worst in the left eye. Ophthalmological examination revealed presence of bilateral CRVO-like retinopathy which raised the hypothesis of hyperviscosity syndrome (HVS). After confirmation of MM diagnosis, plasmapheresis were promptly begun. The right eye regained a good visual acuity and an anatomical restoration already notable 5 days from the first plasmapheresis. Conclusions: Fundoscopy lead to diagnosis of HVS, it is therefore advisable in all patients with MM in order to perform plasmapheresis as soon as possible if necessary. In case of atypical CRVO, haematological and inflammatory causes, should be always ruled out.
Aspirin monotherapy is a suitable standard thromboprophylactic agent following total hip arthroplasty
Authors: Borton Zakk M , Bhangoo Navjot S , Quah Conal SL , Stephen Arthur B , Howard Peter W 
Published: February 2021

Introduction: Aspirin is increasingly recognised as an efficacious thromboprophylactic agent with a superior safety profile compared to alternatives. Following our institution’s previously published experience we implemented a risk-stratified protocol utilising aspirin as standard. We now present retrospective review of standard use of aspirin on fatal pulmonary embolism, all-cause mortality, and venous thromboembolism (VTE) following total hip arthroplasty (THA). Methods: A consecutive series of elective THAs was identified. Prospectively maintained databases were analysed to yield demographic data and identify deaths or readmission data. Patients who died within 90 postoperative days underwent review of the complete medical record. Results: 4204 THAs were included in the study cohort. VTE prophylaxis prescription was available in 3805. 2560 received aspirin (67.3%), 1049 enoxaparin (27.6%) and 193 warfarin (5.1%); there were no differences in 90-day all-cause mortality ( p = 0.780) or VTE ( p = 1) between groups Conclusion: Our large series continues to demonstrate that aspirin for thromboprophylaxis following THA is effective in risk-stratified patients. Furthermore, we demonstrate that introduction of a departmental protocol establishing aspirin as standard practice was not associated with increased mortality or incidence of thromboembolism. Taken in conjunction with our previous cohort our series encompasses 11,420 consecutive THAs. There has been a single death following fatal PE in the aspirin group (0.02%) compared to 5 in the LMWH group (0.2%) and 1 in the warfarin group (0.06%). We join calls for large-scale randomised controlled trials to elucidate the place of aspirin in VTE prevention following hip arthroplasty.
Local infiltration analgesia does not have benefits in fast-track hip arthroplasty programmes: a double-blind, randomised, placebo-controlled, clinical trial
Authors: Bernaus Marti , Novellas Marga , Bartra Agustí , Núñez Jorge H , Anglès Francesc 
Published: February 2021

Background: Multimodal analgesia regimes including local infiltration analgesia (LIA) have been successfully applied in fast-track hip arthroplasty programmes. LIA’s contribution to the analgesic effect in hip arthroplasty has been questioned. Our study sought to determine the analgesic efficacy of LIA in THA surgery in a fast-track programme. Methods: Patients diagnosed with hip osteoarthritis scheduled for arthroplasty were randomised to receive LIA (120 ml ropivacaine 0.2% plus epinephrine 0.5 µ/ml) or saline as a part of a multimodal analgesia regime. The surgical team, the nursing staff, and patients were all blinded regarding patient allocation throughout the study. The primary outcome was pain assessed as a continuous variable using the visual analogue scale (VAS) at 4, 8, 24 and 48 hours postoperatively. Secondary outcomes included the amount of analgesic rescue consumption, complications and length of hospital stay. Results: A total of 63 patients were interviewed and agreed to participate in the study. No statistically significant differences were found between groups for pain measurements at 4, 8, 12, 24 and 48 hours after surgery. There were also no differences in rescue medication consumption, complications, or length of stay. Conclusions: Our results suggest LIA (ropivacaine plus epinephrine, single shot) has no effect in pain management and has not shown benefits for early ambulation in primary THA surgery. Further research is needed to establish the optimal multimodal analgesia regime for THA fast-track programmes. Clinical trial registration: Clinicaltrials.gov (NCT03513276).
Implementing a quality framework for storing emergency department point-of-care ultrasound examinations on a picture archiving and communication system
Authors: Mani Nick 
Published: February 2021

Introduction There is a lack of a quality framework in most emergency departments despite the set national standards for storing point-of-care ultrasound (PoCUS) examinations. To explore and address this problem, a quality improvement project was undertaken in an emergency department. Methods and interventions: A consecutive sample of the most recent PoCUS examinations in a district general hospital emergency department in the United Kingdom was audited. None was handled and stored on the picture archiving and communication system (PACS). Quality improvement project tools, such as plan, do, study, act (PDSA) cycles, were utilised to analyse and improve practice. The PoCUS machine was connected to the local area network to allow transfer of images to PACS. For requesting and booking the examinations, specific requests on the integrated clinical environment were created akin to the computerised radiology information system codes. Other interventions were introduced such as a reporting proforma. The success of the quality improvement project depended partly on key stakeholders such as emergency department consultants, radiology staff and PACS teams. The number of stored examinations on PACS was the main process measure, but others were also considered, for example reporting and documentation. Results Over a 10-month period (September 2018–July 2019), there were three PDSA cycles, which included a total of 195 performed emergency department PoCUS examinations. The implemented quality framework led to 90% of examinations stored to PACS, with a mean of 63%. No negative impacts were reported, and feedback was positive. Conclusion Implementing a quality framework for storing emergency department PoCUS examinations on PACS is feasible and significantly improves practice. Further work is required to sustain and improve the process.
Radial shortening, bone grafting and vascular pedicle implantation versus radial shortening alone in Kienböck’s disease
Authors: Said Elsayed , Addosooki Ahmad , Assaghir Yasser , Ahmed Ahmed , Tammam Hamdy 
Published: February 2021

We prospectively compared outcomes of two surgical procedures for Stage II and IIIa Kienböck’s disease with negative ulnar variance. Group I (13 wrists) was treated with radial shortening alone, and Group II (14 wrists) with combined radial shortening, bone grafting and implantation of a vascular pedicle. At follow-up 3 to 7 years (mean 4.5) after operation, scores by the Quick version of the Disability of Arm, Shoulder and Hand questionnaire and scores for pain and grip strength were significantly better in Group II, but the differences were rather small. On MRI all patients in Group II had revascularization, but 11 patients in Group I had not. We conclude that combining radial shortening with the revascularization procedure improved revascularization. Clinically, both treatments were efficient, and the additional bone grafting and vascular pedicle insertion did not greatly improve function, although statistically there were differences in several follow-up variables. This study also could not identify the independent role of the three procedures (drilling, grafting and artery insertion) in Group II, because decompression or bone grafting alone may be effective. Level of evidence: II
Challenging conventional karyotyping by next-generation karyotyping in 281 intensively treated patients with AML
Authors: Mareschal Sylvain , Palau Anna , Lindberg Johan , Ruminy Philippe , Nilsson Christer , Bengtzén Sofia , Engvall Marie , Eriksson Anna , Neddermeyer Anne , Marchand Vinciane , Jansson Monika , Björklund My , Jardin Fabrice , Rantalainen Mattias , Lennartsson Andreas , Cavelier Lucia , Grönberg Henrik , Lehmann Sören 
Published: February 2021

AbstractAlthough copy number alterations (CNAs) and translocations constitute the backbone of the diagnosis and prognostication of acute myeloid leukemia (AML), techniques used for their assessment in routine diagnostics have not been reconsidered for decades. We used a combination of 2 next-generation sequencing–based techniques to challenge the currently recommended conventional cytogenetic analysis (CCA), comparing the approaches in a series of 281 intensively treated patients with AML. Shallow whole-genome sequencing (sWGS) outperformed CCA in detecting European Leukemia Net (ELN)–defining CNAs and showed that CCA overestimated monosomies and suboptimally reported karyotype complexity. Still, the concordance between CCA and sWGS for all ELN CNA–related criteria was 94%. Moreover, using in silico dilution, we showed that 1 million reads per patient would be enough to accurately assess ELN-defining CNAs. Total genomic loss, defined as a total loss ≥200 Mb by sWGS, was found to be a better marker for genetic complexity and poor prognosis compared with the CCA-based definition of complex karyotype. For fusion detection, the concordance between CCA and whole-transcriptome sequencing (WTS) was 99%. WTS had better sensitivity in identifying inv(16) and KMT2A rearrangements while showing limitations in detecting lowly expressed PML-RARA fusions. Ligation-dependent reverse transcription polymerase chain reaction was used for validation and was shown to be a fast and reliable method for fusion detection. We conclude that a next-generation sequencing–based approach can replace conventional CCA for karyotyping, provided that efforts are made to cover lowly expressed fusion transcripts.
Oligosaccharides increase the genotoxic effect of colibactin produced by pks+ Escherichia coli strains
Authors: Oliero Manon , Calvé Annie , Fragoso Gabriela , Cuisiniere Thibault , Hajjar Roy , Dobrindt Ulrich , Santos Manuela M. 
Published: February 2021

Abstract
Background
Colibactin is a genotoxin that induces DNA double-strand breaks that may lead to carcinogenesis and is produced by Escherichia coli strains harboring the pks island. Human and animal studies have shown that colibactin-producing gut bacteria promote carcinogenesis and enhance the progression of colorectal cancer through cellular senescence and chromosomal abnormalities. In this study, we investigated the impact of prebiotics on the genotoxicity of colibactin-producing E. coli strains Nissle 1917 and NC101.

Methods
Bacteria were grown in medium supplemented with 20, 30 and 40 mg/mL of prebiotics inulin or galacto-oligosaccharide, and with or without 5 μM, 25 μM and 125 μM of ferrous sulfate. Colibactin expression was assessed by luciferase reporter assay for the clbA gene, essential for colibactin production, in E. coli Nissle 1917 and by RT-PCR in E. coli NC101. The human epithelial colorectal adenocarcinoma cell line, Caco-2, was used to assess colibactin-induced megalocytosis by methylene blue binding assay and genotoxicity by γ-H2AX immunofluorescence analysis.

Results
Inulin and galacto-oligosaccharide enhanced the expression of clbA in pks+ E. coli. However, the addition of 125 μM of ferrous sulfate inhibited the expression of clbA triggered by oligosaccharides. In the presence of either oligosaccharide, E. coli NC101 increased dysplasia and DNA double-strand breaks in Caco-2 cells compared to untreated cells.

Conclusion
Our results suggest that, in vitro, prebiotic oligosaccharides exacerbate DNA damage induced by colibactin-producing bacteria. Further studies are necessary to establish whether oligosaccharide supplementation may lead to increased colorectal tumorigenesis in animal models colonized with pks+ E. coli.