bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Proposed Unified Mitotic Chromosome Architecture
Authors: Sedat John , McDonald Angus , Kasler Herbert , Verdin Eric , Cang Hu , Arigovindan Muthuvel , Murre Cornelis , Elbaum Michael 
Published: October 2021

AbstractA molecular architecture is proposed for an example mitotic chromosome, human Chromosome 10. This architecture is built on a previously described interphase chromosome structure based on Cryo-EM cellular tomography (1), thus unifying chromosome structure throughout the complete mitotic cycle. The basic organizational principle, for mitotic chromosomes, is specific coiling of the 11-nm nucleosome fiber into large scale approximately 200 nm structures (a Slinky (2, motif cited in 3) in interphase, and then further modification and subsequent additional coiling for the final structure. The final mitotic chromosome architecture accounts for the dimensional values as well as the well known cytological configurations. In addition, proof is experimentally provided, by digital PCR technology, that G1 T-cell nuclei are diploid, thus one DNA molecule per chromosome. Many nucleosome linker DNA sequences, the promotors and enhancers, are suggestive of optimal exposure on the surfaces of the large-scale coils.Significance StatementThe significance of this proposed mitotic chromosome architecture is that a specific, sequenced chromosome, human Chromosome 10, can be built into a specific architecture that accounts for the dimensional values and cytological descriptions, a first time result. Since this molecular architecture is an extension of the interphase chromosome structure, a coiling of the 11-nm nucleosome fiber with further coiling, a unifying molecular structure motif is present throughout the entire mitotic cycle, interphase through mitosis.
Nuclear morphogenesis: forming a heterogeneous nucleus during embryogenesis
Authors: Tsai Albert , Crocker Justin 
Published: October 2021

AbstractAn embryo experiences progressively complex spatial and temporal patterns of gene expression that guide the morphogenesis of its body plan as it matures. Using super-resolution fluorescence microscopy in Drosophila melanogaster embryos, we observed a similar increase in complexity in the nucleus: the spatial distributions of transcription factors became increasingly heterogeneous as the embryo matured. We also observed a similar trend in chromatin conformation with the establishment of specific histone modification patterns. However, transcription sites of specific genes had distinct local preferences for histone marks separate from the average nuclear trend, depending on the time and location of their expression. These results suggest that reconfiguring the nuclear environment is an integral part of embryogenesis and that the physical organization of the nucleus is a key element in developmental gene regulation.Summary statementWe observed spatial rearrangements in the nucleus during embryo development, progressively forming a heterogeneous nuclear environment, paralleling the increasing complexity of the embryo body as morphogenesis progresses.
A mechanism for exocyst-mediated tethering via Arf6 and PIP5K1C driven phosphoinositide conversion
Authors: Maib Hannes , Murray David H. 
Published: October 2021

AbstractPolarized trafficking is necessary for the development of eukaryotes and is regulated by a conserved molecular machinery. Late steps of cargo delivery are mediated by the exocyst complex, which integrates lipid and protein components to tether vesicles for plasma membrane fusion. However, the molecular mechanisms of this process are poorly defined. Here, we reconstitute functional octameric human exocyst, demonstrating the basis for holocomplex coalescence and biochemically stable subcomplexes. We determine that each subcomplex independently binds to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), which is minimally sufficient for membrane tethering. Through reconstitution and epithelial cell biology experiments, we show that Arf6-mediated recruitment of the lipid kinase PIP5K1C rapidly converts phosphatidylinositol 4-phosphate (PI(4)P) to PI(4,5)P2, driving exocyst recruitment and membrane tethering. These results provide a molecular mechanism of exocyst-mediated tethering and a unique functional requirement for phosphoinositide signaling on latestage vesicles in the vicinity of the plasma membrane.
Knowledge creation and external consultants during ERP implementation: an interpretive study
Authors: Dutta Subhasish , Kumar J. Ajith 
Published: October 2021

PurposePrevious research has investigated knowledge transfer and the external consultant's role in it, during enterprise resource planning (ERP) implementation. In this study, the authors explore the processes through which knowledge creation happens during ERP implementation and how external consultants help operationalize the same.Design/methodology/approachThe authors engaged the SECI model of the theory of organizational knowledge creation and conducted an interpretive study that interviewed 14 ERP implementation experts in India. The interviews were transcribed and then analyzed using the ATLAS.ti package.FindingsThe findings reveal fine insights into how knowledge creation takes place during ERP implementation through the four modes of knowledge conversion – socialization (S), externalization (E), combination (C) and internalization (I). The external consultants aid in operationalizing this knowledge creation process through five types of knowledge-related activities: (I) importing external knowledge, (D) discovering internal knowledge, (E) extricating confined knowledge, (A) activating flows of knowledge and (S) solving problems using knowledge. These activities can be represented by the acronym, I-D-E-A-S.Originality/valueThe study makes two overarching contributions: (1) offering an interpretation and description of ERP implementation as a knowledge creation process and (2) extending the understanding of the external consultants' role during ERP implementation to include new knowledge creation.
Breaking the mould: achieving high-volume production output with additive manufacturing
Authors: Huang Yuan , Eyers Daniel R. , Stevenson Mark , Thürer Matthias 
Published: October 2021

PurposeThe study aims to examine a discrepant industrial case that demonstrates how to achieve economies of scale with additive manufacturing (AM), thereby expanding the scope of AM beyond high-variety, customised production contexts.Design/methodology/approachAbductive reasoning is applied to analyse a case of using AM to compete with conventional production, winning a contract to supply 7,700,000 products. Comparing this case to existing theories and contemporary practices reveals new research directions and practical insights.FindingsEconomies of scale were realised through a combination of technological innovation and the adoption of operations management practices atypical of AM shops (e.g. design for volume, low-cost resource deployment and material flow optimisation). The former improved AM process parameters in terms of time, cost and dependability; the latter improved the entire manufacturing system, including non-AM operations/resources. This system-wide improvement has been largely overlooked in the literature, where AM is typically viewed as a disruptive technology that simplifies manufacturing processes and shortens supply chains.Originality/valueIt is empirically shown that an AM shop can achieve economies of scale and compete with conventional manufacturing in high-volume, standardised production contexts.
Game play and the effectiveness of sponsor signage: visual attention to brand messages in live sport broadcasts
Authors: Boronczyk Felix , Rumpf Christopher , Breuer Christoph 
Published: October 2021

PurposeTechnological innovations could allow for real-time control of sponsor exposure during sport broadcasts to increase the capacity of sponsor messages to attract attention. While such an approach requires knowledge on the interplay between in-game events and viewers' attention to sponsor signage previous studies have disregarded factors pertaining to the competition itself. To address this gap, this paper investigates the impact of game-related variables on the allocation of viewer attention in live broadcasts.Design/methodology/approachEye tracking is used to examine the impact of variations in score, ball position and ball possession on soccer fans' attention to sponsor messages during broadcasts. In total, the data comprise n = 36,604 second-by-second observations from 11 participants and games.FindingsLogistic regression analysis reveals game time effects and significant interaction effects between possession and the score as well as the ball position variable. Sponsors receive less attention if the attacking team controls the ball near the opponents' goal, particularly if the viewers' preferred team is in possession.Practical implicationsProperty owners and sponsors can exploit these findings to determine the value of sponsor brand exposure more precisely. New systems could further increase message effectiveness through adjustments of sponsor exposure based on real time match event data.Originality/valueThis study is the first to explore the influence of game-related variables on attention to sponsors. By employing live broadcasts in a near-realistic setting, it further addresses a gap in the literature and adds to the knowledge on sponsor message processing.
Feeling a historic city: Porto landscape through the eyes of residents and visitors
Authors: Vaz de Freitas Isabel , Sousa Cristina , Ramazanova Makhabbat , Albuquerque Helena 
Published: October 2021

Purpose
This paper aims to monitor the urban landscape through the perceptions of residents and visitors, identifying features that cause visual impacts and providing insights for landscape management decision-makers. Captured impressions about the city whilst moving around are important to assess the satisfaction of city residents and city visitors through key elements, such as directional signage, outdoor advertising, restaurants’ outdoor terrace furniture, urban furniture, green spaces, traffic, cleaning, pedestrian areas, visitor flow and conservation of monuments, museums and buildings.

Design/methodology/approach
A survey was applied in the historical Porto city centre (Portugal) to understand residents’ and visitors’ perceptions of the city landscape and assess the differences between these two groups. Cronbach’s alpha was used to assess the reliability of the constructs (dimensions) of urban landscape. Non-parametric Mann-Whitney test was used to find significant differences in the perceptions of residents and visitors.

Findings
The results suggest significant differences in the perceptions. Residents value more two constructs of the landscape (heritage conservation and transport mobility) whilst visitors value more other two (pedestrian mobility and aesthetic quality). It is showed that residents have a strong sense of place and are concerned with the conservation of historical heritage. Visitors are more concerned with dimensions intrinsically related to tourism.

Originality/value
This research allowed to fill a gap found in the literature, namely, the importance of considering the perceptions of different actors in the urban landscape monitoring. These results are an important contribution for local authorities to understand the value of urban landscape elements from the perspective of residents and visitors. This study opened the possibility of comparing the results from different historical cities centres.
The economic impacts of COVID-19 on the city tourism-related services; the case of Tehran
Authors: Parvin Mohammad , Tavakolinia Jamileh , Mosammam Hassan Mohammadian , Razavian Mohammadtaghi 
Published: October 2021

Purpose
The COVID-19 pandemic has had an extremely destructive impact on the tourism and hospitality industry. However, there is a gap in the literature on measuring the economic impact of the pandemic on employees of urban tourism industries by class. Accordingly, this paper aims to investigate the perceived economic impact of the COVID-19 by workers of urban tourism-related services in Tehran.

Design/methodology/approach
Based on stratified sampling, a Web-based survey was carried out among 383 employees of tourism industries. To measuring the perceived impact and evaluate statistically significant differences, one samples t-test, one-way ANOVA and post hoc test were used.

Findings
The study findings revealed that employees of tourism-related services perceived that COVID-19 outbreak has a huge negative impact on the tourism industries in terms of earnings reduction. However, the perceived impact on job losses and reduction of hours of work was non-monotonic.

Originality/value
Identifying the negative effects of the COVID-19 outbreak on city tourism by industries and their subclasses can contribute to more effective interventions to support and recover the tourism industries. In other words, it is essential to prioritize support for different class of services, to fair allocation of bailout monies and to improve their resilience against crises and shocks.
Impact of digital technology on velocity of B2B buyer-supplier relationship development
Authors: Kauffman Ralph , Pointer Lucille 
Published: October 2021

PurposeThis study aims to examine how the widespread adoption of digital technology (DT) in business-to-business (B2B) markets affects and, in particular, increases the velocity of relationship development over time.Design/methodology/approachA literature search was conducted to develop propositions concerning DT’s effect on the various stages of an existing B2B buyer-seller relationship development model. A group of 55 experienced practitioners was used to obtain reactions to the propositions.FindingsDT affects buyer-seller relationship development by reducing the time needed to initiate and advance through sequential relationship stages. Agility in the decision-making process fosters stronger inter-firm relationships and influences other important attributes of B2B relationships, such as organizational commitment, organizational embeddedness, trust and value creation.Research limitations/implicationsA broader, more diverse sample of commercial buyers and sellers is required to permit testing the generalizability of the study’s findings.Practical implicationsDT affects the speed and agility of B2B relationship formation regardless of stage. As DT evolves in the age of Industry 4.0, an understanding of the effects of DT will aid managers in assessing ways to leverage its potential and apply appropriate DT strategies throughout the B2B relationship process to capitalize on current and future business opportunities. Firms need to explore the positive and negative effects of the digital revolution on managers within their supply chain networks.Originality/valueTo the best of the authors’ knowledge, this is the first study that specifically addresses DT’s impact during the specific stages of the relationship development process.
Development of complexity management strategies for construction projects
Authors: Kermanshachi Sharareh , Nipa Thahomina Jahan , Dao Bac 
Published: October 2021

Purpose
The purpose of this study is to ascertain and list the most effective management strategies in efficiently handling the project complexities to enhance the performance of the project.

Design/methodology/approach
To fulfill the aim of this study, a comprehensive literature review was conducted, and the qualitative Delphi technique in two rounds was applied. Participants of the Delphi technique consisted of 12 subject matter experts (SMEs) with cumulative experience of 250 years in working in construction projects. In the first round of the Delphi technique, SMEs were asked to provide complexity management strategies to address the complexities due to 37 complexity indicators (CIs) under 11 complexity categories. In the second round of the Delphi technique, SMEs identified the top three management strategies for each of the 37 CIs.

Findings
This study collected the outcome of the two-round Delphi technique and based on the output developed the list of strategies to manage complexities related to each indicator. For example, establishing a well-informed governance team, assigning a Project Manager (PM) when the number of projects is more than one in an organization, and assigning a PM efficient enough to communicate with higher authority effectively will help in managing complexity that arises due to faulty assessment of the influence of a project on the organization’s overall success.

Originality/value
This study will help practitioners in effectively managing the project complexities, and thus will reduce the monetary loss associated with project complexities.
Exploring e-complaint method: learning from the Malaysian Polytechnic institutions
Authors: Ismail Zul-Atfi 
Published: October 2021

Purpose
The conventional e-complaint method provides a platform that contains multiple data sources of an infrastructure project, including building facilities, associated asset documentation of the asset. However, the conventional e-complaint method practised by the Development and Facilities Management Unit (UPPF) faces issues due to management deficiencies and negligent staff to handle operations and maintenance processes within facilities management at Malaysian Polytechnic. This paper aims to identify the different maintenance management system (MMS) approaches using information and communication technology that is equipped with decision-making processes.

Design/methodology/approach
This research presents eight cases of the use of e-complaint in major facilities management platforms in Malaysian Polytechnic, identifying the major problems using the conventional e-complaint method, the current technologies realised, as well as the procedures for learning and improving e-complaint to the Polytechnic institution. The major conclusion drawn from the interview results was that there is a lack of comprehensive MMSs that integrate operations and maintenance processes within facilities management and software programming to provide guidelines for decision-making processes.

Findings
The findings demonstrate that the conventional e-complaint method cannot be used effectively to support the defect diagnosis process during maintenance assessment and development. The case studies further demonstrated that the conventional e-complaint method will be particularly led to inadequate strategic decision-making to analyse information to improve the maintenance project outcomes for Malaysian Polytechnic’s buildings, thereby, ignoring the accuracy and reliability of information for operations and maintenance. New MMS tools are suggested from the findings and lessons learned as a good practice to reduce defect repetition on the design specifications used and construction methods practised.

Originality/value
Due to the increasingly complex nature of projects in terms of size and information technology, realising these current technologies would require a learning process for all stakeholders involved in financial and managing assets. This research proposes stepped change and learning opportunity for maintenance value maximisation and delivery, management and operation efficiency using MMS. The new MMS can potentially transform operational and maintenance processes within facilities management into one of the most sophisticated technologies by accommodating data recordings, such as complaints and specific items needed for maintenance through the internet and intranet to facilitate improved execution of Polytechnic’s building activities.
Resident loyalty formation for the survival of African-ethnic restaurants during COVID-19: the adaptive capabilities logic
Authors: Otengei Samson Omuudu , Changha George 
Published: October 2021

PurposeThis qualitative research aimed to explore the relationship between adaptive dynamic capabilities and resident loyalty formation among African-ethnic restaurants (AERs) during COVID-19 in East Africa.Design/methodology/approachAnchored on the dynamic capabilities perspective, the study uses eight case studies to obtain data based on restaurant owner-managers' lived experiences.FindingsThe paper presents six factors: (1) sensory quality promise, (2) service personalization declaration, (3) openness of technology adoption, (4) healthy food and safety assurance, (5) authenticity pledge and (6) diversity provision as prerequisites for resident loyalty formation. The findings suggest adaptive capacity as an appropriate alternative for stimulating resident loyalty formation during difficult times.Practical implicationsThe findings help managers in formulating strategies that facilitate residents' display of willingness to revisit and/or recommend others. The local restaurants can now keep modifying and adjusting their practices and processes so as to exhibit the ability to handle customer unique demands during difficult times.Originality/valueThrough this work, a model of adaptive capabilities as enablers of resident loyalty formation is proposed, hence contributing the existing body of knowledge.
Street-level bureaucrats: tensions and challenges in online placemaking
Authors: Breek Pieter , Eshuis Jasper , Hermes Joke 
Published: October 2021

Purpose
Social media have become a key part of placemaking. Placemaking revolves around collaboration between multiple stakeholders, which requires ongoing two-way communication between local government and citizens. Although social media offer promising tools for local governments and public professionals in placemaking, they have not lived up to their potential. This paper aims to uncover the tensions and challenges that social media bring for public professionals at the street level in placemaking processes.

Design/methodology/approach
This study aims to fill this gap with a case study of area brokers engaged in online placemaking in Amsterdam. In total, 14 in-depth semi-structured interviews were conducted, focusing on area brokers’ social media practices, perceptions and challenges. The authors used an open coding strategy in the first phase of coding. In the second phase, the authors regrouped codes in thematic categories with the use of sensitizing concepts derived from the theoretical review.

Findings
The use of social media for placemaking imposes demands on area brokers from three sides: the bureaucracy, the affordances of social media and affective publics. The paper unpacks pressures area brokers are under and the (emotional) labour they carry out to align policy and bureaucratic requirements with adequate communication needed in neighbourhood affairs on social media. The tensions and the multidimensionality of what is required explain the reluctance of area brokers to exploit the potential of social media in their work.

Originality/value
Several studies have addressed the use of social media in placemaking, but all neglected the perspective of street-level bureaucrats who shape the placemaking process in direct contact with citizens.