bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maleic and l-tartaric acids as new anti-sprouting agents for potatoes during storage in comparison to other efficient sprout suppressants
Authors: Bhattacharya Ekta , Mandal Biswas Suparna , Pramanik Panchanan 
Published: October 2021

AbstractInhibiting sprouting of potatoes is an interesting subject needed for potato storage and industry. Sprouting degrades the quality of tuber along with releasing α-solanine and α-chaconine, which are harmful for health. Sprout suppressants, available in the market, are either costly or toxic to both health and environment. So, there is a need for developing countries to explore new sprouting suppressant compound which is cheap, non-toxic and reasonably efficient in comparison to commercial ones. We have established that simple maleic acid and l-tartaric acid are effective sprout suppressing agents. Both can hinder sprouting up to 6 weeks and 4 weeks post treatment respectively at room temperature in dark. These do not affect the quality parameters, retain the moisture content and maintain the stout appearance of the tubers along the total storage period. Thus maleic acid and l-tartaric acid would qualify as alternative, cheap, efficient sprout suppressant for potato storage and processing.
Primary stability and PES/WES evaluation for immediate implants in the aesthetic zone: a pilot clinical double-blind randomized study
Authors: Sanchez-Perez Arturo , Nicolas-Silvente Ana I. , Sanchez-Matas Carmen , Molina-García Silvia , Navarro-Cuellar Carlos , Romanos Georgios E. 
Published: October 2021

AbstractThe use of immediate implants in the aesthetic area is a technique widely used in modern implantology. The characteristics of the patient, the implant, and the surgical procedure used may influence the final results. The aim was to assess whether the implant design affects primary (P.S.) and secondary stability (S.S.), bone level (B.L.), and PES/WES evaluation. Twenty implants with two different designs (n = 10) were immediately placed and randomly located in the upper anterior maxilla with no grafting material. Implant-Stability-Quotient (ISQ), B.L., and Pink-Esthetic-Score/White-Esthetic-Score (PES/WES) were evaluated. Shapiro–Wilk normality test was performed to determine the sample normality, as the data did not follow a normal distribution, the Wilcoxon-Mann–Whitney test was applied (p < 0.05). ISQ was determined at placement (PS): control 59.1 (C.I.54.8–63.3); experimental 62.2(C.I.60.1–64.2) and three months after placement (SS): control 62.2.1 (C.I.53.3–71.0); experimental 67.2(C.I.65.8–68.5). The BL was measured at three months after placement: control 0.38 mm (C.I.− 0.06 to  + 0.83); experimental 0.76 mm (C.I.0.33–1.19) and at 12 months post-loading: control 0.07 mm (C.I.− 0.50–0.65); experimental 0.90 mm (C.I.0.38–1.42). PES/WES values were evaluated for the control group: 15 (C.I.12.68–17.32), and for the experimental group 15.20 (C.I.11.99–18.41). No significant differences were shown between both implant designs. A good grade of osseointegration and primary/secondary stability was achieved, as well as proper maintenance of crestal bone and adequate PES/WES scores. The criteria for selection for the ideal patient for immediate implant placement is essential.ClinicalTrials Protocol ID: NCT04343833.
BRAF mutations may identify a clinically distinct subset of glioblastoma
Authors: McNulty Samantha N. , Schwetye Katherine E. , Ferguson Cole , Storer Chad E. , Ansstas George , Kim Albert H. , Gutmann David H. , Rubin Joshua B. , Head Richard D. , Dahiya Sonika 
Published: October 2021

AbstractGlioblastoma (GBM) is the most common primary malignant brain tumor in adults. Prior studies examining the mutational landscape of GBM revealed recurrent alterations in genes that regulate the same growth control pathways. To this regard, ~ 40% of GBM harbor EGFR alterations, whereas BRAF variants are rare. Existing data suggests that gain-of-function mutations in these genes are mutually exclusive. This study was designed to explore the clinical, pathological, and molecular differences between EGFR- and BRAF-mutated GBM. We reviewed retrospective clinical data from 89 GBM patients referred for molecular testing between November 2012 and December 2015. Differences in tumor mutational profile, location, histology, and survival outcomes were compared in patients with EGFR- versus BRAF-mutated tumors, and microarray data from The Cancer Genome Atlas was used to assess differential gene expression between the groups. Individuals with BRAF-mutant tumors were typically younger and survived longer relative to those with EGFR-mutant tumors, even in the absence of targeted treatments. BRAF-mutant tumors lacked distinct histomorphology but exhibited unique localization in the brain, typically arising adjacent to the lateral ventricles. Compared to EGFR- and IDH1-mutant tumors, BRAF-mutant tumors showed increased expression of genes related to a trophoblast-like phenotype, specifically HLA-G and pregnancy specific glycoproteins, that have been implicated in invasion and immune evasion. Taken together, these observations suggest a distinct clinical presentation, brain location, and gene expression profile for BRAF-mutant tumors. Pending further study, this may prove useful in the stratification and management of GBM.
Spatio-temporal analysis and prediction of malaria cases using remote sensing meteorological data in Diébougou health district, Burkina Faso, 2016–2017
Authors: Bationo Cédric S. , Gaudart Jean , Dieng Sokhna , Cissoko Mady , Taconet Paul , Ouedraogo Boukary , Somé Anthony , Zongo Issaka , Soma Dieudonné D. , Tougri Gauthier , Dabiré Roch K. , Koffi Alphonsine , Pennetier Cédric , Moiroux Nicolas 
Published: October 2021

AbstractMalaria control and prevention programs are more efficient and cost-effective when they target hotspots or select the best periods of year to implement interventions. This study aimed to identify the spatial distribution of malaria hotspots at the village level in Diébougou health district, Burkina Faso, and to model the temporal dynamics of malaria cases as a function of meteorological conditions and of the distance between villages and health centres (HCs). Case data for 27 villages were collected in 13 HCs. Meteorological data were obtained through remote sensing. Two synthetic meteorological indicators (SMIs) were created to summarize meteorological variables. Spatial hotspots were detected using the Kulldorf scanning method. A General Additive Model was used to determine the time lag between cases and SMIs and to evaluate the effect of SMIs and distance to HC on the temporal evolution of malaria cases. The multivariate model was fitted with data from the epidemic year to predict the number of cases in the following outbreak. Overall, the incidence rate in the area was 429.13 cases per 1000 person-year with important spatial and temporal heterogeneities. Four spatial hotspots, involving 7 of the 27 villages, were detected, for an incidence rate of 854.02 cases per 1000 person-year. The hotspot with the highest risk (relative risk = 4.06) consisted of a single village, with an incidence rate of 1750.75 cases per 1000 person-years. The multivariate analysis found greater variability in incidence between HCs than between villages linked to the same HC. The time lag that generated the better predictions of cases was 9 weeks for SMI1 (positively correlated with precipitation variables) and 16 weeks for SMI2 (positively correlated with temperature variables. The prediction followed the overall pattern of the time series of reported cases and predicted the onset of the following outbreak with a precision of less than 3 weeks. This analysis of malaria cases in Diébougou health district, Burkina Faso, provides a powerful prospective method for identifying and predicting high-risk areas and high-transmission periods that could be targeted in future malaria control and prevention campaigns.
Impact of de-ionized water on changes in porosity and permeability of shales mineralogy due to clay-swelling
Authors: Zhang Di , Meegoda Jay N. , da Silva Bruno M. Goncalves , Hu Liming 
Published: October 2021

AbstractHydraulic fracturing is widely applied for economical gas production from shale reservoirs. Still, the swelling of the clay micro/nano pores due to retained fluid from hydraulic fracturing causes a gradual reduction of gas production. Four different gas-bearing shale samples with different mineralogical characteristics were investigated to study the expected shale swelling and reduction in gas permeability due to hydraulic fracturing. To simulate shale softening, these shale samples were immersed in deionized (DI) water heated to 100 °C temperature and subjected to 8 MPa pressure in a laboratory reactor for 72 hours to simulate shale softening. The low-temperature nitrogen adsorption and density measurements were performed on the original and treated shale to determine the changes in micro and nano pore structure. The micro and nano pore structures changed, and the porosity decreased after shale treatment. The porosity decreased by 4% for clayey shale, while for well-cemented shale the porosity only decreased by 0.52%. The findings showed that the initial mineralogical composition of shale plays a significant role in the change of micro and nano pores and the pore structure alteration due to retained fluid from hydraulic fracturing. A pore network model is used to simulate the permeability of shale used in this study. To define pore structure properties, specific factors such as porosity, pore size, pore throat distribution, and coordination number were used. Furthermore, the anisotropy characteristics of shale were integrated into the model via a coordination number ratio. Finally, the change in permeability due to shale softening was determined and compared with untreated with the progress of shale softening. The simulation showed that the permeability of Longmaxi shale could decrease from 3.82E–16 m2 to 4.71E–17 m2 after treatment.
Clinical phenotypes from fatal cases of acute respiratory distress syndrome caused by pneumonia
Authors: Ichikado Kazuya , Kawamura Kodai , Johkoh Takeshi , Fujimoto Kiminori , Shintani Ayumi , Hashimoto Satoru , Eguchi Yoshitomo , Yasuda Yuko , Anan Keisuke , Shingu Naoki , Sakata Yoshihiko , Hisanaga Junpei , Nitawaki Tatsuya , Iio Miwa , Sekido Yuko , Nishiyama Kenta , Nakamura Kazunori , Suga Moritaka , Ichiyasu Hidenori , Sakagami Takuro 
Published: October 2021

AbstractThere have been no report of objective clinical characteristics or prognostic factors that predict fatal outcome of acute respiratory distress syndrome (ARDS) since the Berlin definition was published. The aim of this study is to identify clinically available predictors that distinguish between two phenotypes of fatal ARDS due to pneumonia. In total, 104 cases of Japanese patients with pneumonia-induced ARDS were extracted from our prospectively collected database. Fatal cases were divided into early (< 7 days after diagnosis) and late (≥ 7 days) death groups, and clinical variables and prognostic factors were statistically evaluated. Of the 50 patients who died within 180 days, 18 (36%) and 32 (64%) were in the early (median 2 days, IQR [1, 5]) and late (median 16 days, IQR [13, 29]) death groups, respectively. According to multivariate regression analyses, the APACHE II score (HR 1.25, 95%CI 1.12–1.39, p < 0.001) and the disseminated intravascular coagulation score (HR 1.54, 95%CI 1.15–2.04, p = 0.003) were independent prognostic factors for early death. In contrast, late death was associated with high-resolution computed tomography (HRCT) score indicating early fibroproliferation (HR 1.28, 95%CI 1.13–1.42, p < 0.001) as well as the disseminated intravascular coagulation score (HR 1.24, 95%CI 1.01–1.52, p = 0.039). The extent of fibroproliferation on HRCT, and the APACHE II scores along with coagulation abnormalities, should be considered for use in predictive enrichment and personalized medicine for patients with ARDS due to pneumonia.
Stem cells from human exfoliated deciduous teeth attenuate mechanical allodynia in mice through distinct from the siglec-9/MCP-1-mediated tissue-repairing mechanism
Authors: Hayashi Yoshinori , Kato Hiroki , Nonaka Kazuaki , Nakanishi Hiroshi 
Published: October 2021

AbstractThe effects of stem cells from human exfoliated deciduous teeth (SHED) on mechanical allodynia were examined in mice. A single intravenous injection of SHED and conditioned medium from SHED (SHED-CM) through the left external jugular vein significantly reversed the established mechanical allodynia induced by spinal nerve transection at 6 days after injection. SHED or SHED-CM significantly decreased the mean numbers of activating transcription factor 3-positive neurons and macrophages in the ipsilateral side of the dorsal root ganglion (DRG) at 20 days after spinal nerve transection. SHED or SHED-CM also suppressed activation of microglia and astrocytes in the ipsilateral side of the dorsal spinal cord. A single intravenous injection of secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 had no effect on the established mechanical allodynia, whereas a single intravenous injection of protein component(s) contained in SHED-CM with molecular weight of between 30 and 50 kDa reversed the pain. Therefore, it may be concluded that protein component(s) with molecular mass of 30–50 kDa secreted by SHED could protect and/or repair DRG neurons damaged by nerve transection, thereby ameliorating mechanical allodynia.
Respiratory viral infections in pragmatically selected adults in intensive care units
Authors: Cia Cong-Tat , Lin I-Ting , Lee Jen-Chieh , Tsai Huey-Pin , Wang Jen-Ren , Ko Wen-Chien 
Published: October 2021

AbstractRespiratory viruses can be detected in 18.3 to 48.9% of critically ill adults with severe respiratory tract infections (RTIs). The present study aims to assess the clinical significance of respiratory viruses in pragmatically selected adults in medical intensive care unit patients and to identify factors associated with viral respiratory viral tract infections (VRTIs). We conducted a prospective study on critically ill adults with suspected RTIs without recognized respiratory pathogens. Viral cultures with monoclonal antibody identification, in-house real-time polymerase chain reaction (PCR) for influenza virus, and FilmArray respiratory panel were used to detect viral pathogens. Multivariable logistic regression was applied to identify factors associated with VRTIs. Sixty-four (40.5%) of the included 158 critically ill adults had respiratory viruses detected in their respiratory specimens. The commonly detected viruses included influenza virus (20), followed by human rhinovirus/enterovirus (11), respiratory syncitial virus (9), human metapneumovirus (9), human parainfluenza viruses (8), human adenovirus (7), and human coronaviruses (2). The FilmArray respiratory panel detected respiratory viruses in 54 (34.6%) patients, but showed negative results for seven of 13 patients with influenza A/H3 infection. In the multivariable logistic regression model, patient characters associated with VRTIs included those aged < 65 years, household contact with individuals with upper RTI, the presence of fever, cough with sputum production, and sore throat. Respiratory viruses were not uncommonly detected in the pragmatically selected adults with critical illness. The application of multiplex PCR testing for respiratory viruses in selected patient population is a practical strategy, and the viral detection rate could be further improved by the patient characters recognized in this study.
Quantifying the role of ocean coupling in Arctic amplification and sea-ice loss over the 21st century
Authors: Chemke Rei , Polvani Lorenzo M. , Kay Jennifer E. , Orbe Clara 
Published: October 2021

AbstractThe enhanced warming of the Arctic, relative to other parts of the Earth, a phenomenon known as Arctic amplification, is one of the most striking features of climate change, and has important climatic impacts for the entire Northern Hemisphere. Several mechanisms are believed to be responsible for Arctic amplification; however, a quantitative understanding of their relative importance is still missing. Here, using ensembles of model integrations, we quantify the contribution of ocean coupling, both its thermodynamic and dynamic components, to Arctic amplification over the 20th and 21st centuries. We show that ocean coupling accounts for ~80% of the amplification by 2100. In particular, we show that thermodynamic coupling is responsible for future amplification and sea-ice loss as it overcomes the effect of dynamic coupling which reduces the amplification and sea-ice loss by ~35%. Our results demonstrate the utility of targeted numerical experiments to quantify the role of specific mechanisms in Arctic amplification, for better constraining climate projections.
Comprehensive RNA analysis of CSF reveals a role for CEACAM6 in lung cancer leptomeningeal metastases
Authors: Li Yingmei , Polyak Dina , Lamsam Layton , Connolly Ian David , Johnson Eli , Khoeur Lina Khav , Andersen Stephanie , Granucci Monica , Stanley Geoff , Liu Boxiang , Nagpal Seema , Hayden Gephart Melanie 
Published: October 2021

AbstractNon-small cell lung cancer (NSCLC) metastatic to the brain leptomeninges is rapidly fatal, cannot be biopsied, and cancer cells in the cerebrospinal fluid (CSF) are few; therefore, available tissue samples to develop effective treatments are severely limited. This study aimed to converge single-cell RNA-seq and cell-free RNA (cfRNA) analyses to both diagnose NSCLC leptomeningeal metastases (LM), and to use gene expression profiles to understand progression mechanisms of NSCLC in the brain leptomeninges. NSCLC patients with suspected LM underwent withdrawal of CSF via lumbar puncture. Four cytology-positive CSF samples underwent single-cell capture (n = 197 cells) by microfluidic chip. Using robust principal component analyses, NSCLC LM cell gene expression was compared to immune cells. Massively parallel qPCR (9216 simultaneous reactions) on human CSF cfRNA samples compared the relative gene expression of patients with NSCLC LM (n = 14) to non-tumor controls (n = 7). The NSCLC-associated gene, CEACAM6, underwent in vitro validation in NSCLC cell lines for involvement in pathologic behaviors characteristic of LM. NSCLC LM gene expression revealed by single-cell RNA-seq was also reflected in CSF cfRNA of cytology-positive patients. Tumor-associated cfRNA (e.g., CEACAM6, MUC1) was present in NSCLC LM patients’ CSF, but not in controls (CEACAM6 detection sensitivity 88.24% and specificity 100%). Cell migration in NSCLC cell lines was directly proportional to CEACAM6 expression, suggesting a role in disease progression. NSCLC-associated cfRNA is detectable in the CSF of patients with LM, and corresponds to the gene expression profile of NSCLC LM cells. CEACAM6 contributes significantly to NSCLC migration, a hallmark of LM pathophysiology.
Raman microscopy-based quantification of the physical properties of intracellular lipids
Authors: Uematsu Masaaki , Shimizu Takao 
Published: October 2021

AbstractThe physical properties of lipids, such as viscosity, are homeostatically maintained in cells and are intimately involved in physiological roles. Measurement of the physical properties of plasma membranes has been achieved primarily through chemical or genetically encoded fluorescent probes. However, since most probes target plasma membranes, physical properties of lipids in intracellular organelles, including lipid droplets (LDs) are yet to be analyzed. Here, we present a novel Raman microscopy-based approach for quantifying the physical properties of intracellular lipids under deuterium-labeled fatty acid treatment conditions. Focusing on the fact that Raman spectra of carbon-deuterium vibration are altered depending on the surrounding lipid species, we quantitatively represented the physical properties of lipids as the gauche/trans conformational ratio of the introduced labeled fatty acids, which can be used as an indicator of viscosity. Intracellular Raman imaging revealed that the gauche/trans ratio of cytosolic regions was robustly preserved against perturbations attempting to alter the lipid composition. This was likely due to LDs functioning as a buffer against excess gauche/trans ratio, beyond its traditional role as an energy storage organelle. Our novel approach enables the observation of the physical properties of organelle lipids, which is difficult to perform with conventional probes, and is useful for quantitative assessment of the subcellular lipid environment.
Tuning the aggregation behaviour of BN-coronene diimides with imide substituents and their performance in devices (OLEDs and OFETs)
Authors: Hoffmann Jonas , Geffroy Bernard , Jaques Emmanuel , Hissler Muriel , Staubitz Anne 
Published: October 2021

The lateral CC-π-extension of perylene diimides (PDIs) to coronene diimides (CDIs) leads to undesired effects such as the decrease of the absorption and a hypsochromic shift. This could be overcome by lateral BN-annulation of PDIs to form superior BNCDIs.
Cross‐scale feature fusion connection for a YOLO detector
Authors: Ruan Zhongling , Wang Hao , Cao Jianzhong , Zhang Hongbo 
Published: October 2021

AbstractMulti‐scale feature fusion is often used to address the issue of scale variations in object detection. However, most of the proposed network architectures only combine the features of two adjacent levels sequentially, so the first fusion nodes in both top‐down and bottom‐up pathways must be blank nodes that only have one input with no feature fusion. In this work, cross‐scale feature fusion connection (CFFC) is proposed which aims to enhance the entire feature hierarchy by propagating the features of each level more efficiently. The proposed method reuses and aggregates all the features of other scales to the blank nodes in both top‐down and bottom‐up pathways. Furthermore, the authors remove the 1 × 1 convolutional layer and replace the shortcut with concatenation before fusing multiple features. These concatenated feature maps are then supervised by the channel attention block at the fusion nodes. This modification allows the network to learn the important degree of each level in concatenated feature maps along the channel dimension. It is also observed that the proposed method alleviates the inconsistency in feature pyramids with fewer parameters. The performance of a YOLO object detector equipped with the proposed method on the COCO test‐dev 2017 is evaluated. The results show that the proposed method outperforms other architectures presented in the literature.
Substrate integrated waveguide based class‐F power amplifier
Authors: Yang Fei , Yu Hongxi , Li Jun , Xu Kai‐Da , Zhang Anxue , Jin Zhonghe 
Published: October 2021

AbstractThis work outlines the design, implementation, and experimental results of a Class‐F power amplifier (PA) with a novel output matching topology by utilizing substrate integrated waveguide (SIW). The design methodology is first proposed with one highly integrated SIW matching structure. The impedances at fundamental, second harmonic and third harmonic frequencies are analysed to explain good impedance matching for the PA as well as the isolation mechanism of the direct current (DC) and radio frequency (RF). For demonstration, an S‐band 10‐W Class‐F PA is fabricated and tested. The simulation and measurement results agree very well with each other, which verifies the proposed technology of SIW output matching. To the authors’ knowledge, this is the first time to use a highly integrated SIW matching in Class‐F PA, which simplifies the output matching for third‐order harmonics and DC/RF isolation structures.
„Eine integrativmedizinische Praxis und EbM passen gut zueinander“
Authors: Kühne Rolf 
Published: October 2021

SummaryKomplementärmedizin und Naturheilverfahren sind primär ein Gewinn für die Patient*innen und das ist in jedem Fall auch ein Gewinn für die Praxis.Ein Gespräch mit Dr. Rolf Kühne