bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do Naturally Occurring Antioxidants Protect Against Neurodegeneration
of the Dopaminergic System? A Systematic Revision in Animal Models of
Parkinson's Disease
Authors: Costas Carmen , Faro Lilian R.F. 
Published: May 2021

Abstract:
Parkinson’s disease (PD) is the second most common neurodegenerative disease and is
characterized by a significant decrease in dopamine levels, caused by progressive degeneration of
the dopaminergic neurons in the nigrostriatal pathway. Multiple mechanisms have been implicated
in its pathogenesis, including oxidative stress, neuroinflammation, protein aggregation, mitochondrial
dysfunction, insufficient support for neurotrophic factors and cell apoptosis. The absence
of treatments capable of slowing or stopping the progression of PD has increased the interest
in the natural antioxidant substances present in the diet, since they have multiple beneficial properties
and it is possible that they can influence the mechanisms responsible for the dysfunction
and death of dopaminergic neurons. Thus, the purpose of this systematic review is to analyze the
results obtained in a set of studies carried out in the last years, which describe the neuroprotective,
antioxidant and regenerative functions of some naturally occurring antioxidants in experimental
models of PD. The results show that the exogenous no enzymatic antioxidants can significantly
modify the biochemical and behavioral mechanisms that contribute to the pathophysiology of
Parkinsonism in experimental animals. Therefore, it is possible that they may contribute to effective
neuroprotection by providing a significant improvement in neuropathological markers. In conclusion,
the results of this review suggest that exogenous antioxidants can be promising therapeutic
candidates for the prevention and treatment of PD.
Hindering the Synchronization Between miR-486-5p and H19 lncRNA by Hesperetin
Halts Breast Cancer Aggressiveness Through Tuning ICAM-1
Authors: Abdallah Ramah M. , Elkhouly Aisha M. , Soliman Raghda A. , El Mechawy Nehad , El Sebaei Ahmed , Motaal Amira A. , El-Askary Hesham , Youness Rana A. , Assal Reem A. 
Published: May 2021

Background:
Recently, a novel crosstalk between non-coding RNAs (ncRNAs) has been casted.
However, this has been seldom investigated in metastatic BC (mBC). H19 and miR-486-5p role in mBC are
controversial. ICAM-1 is a recently recognized metastatic engine in mBC. Natural compounds were recently
found to alter ncRNAs/target circuits. Yet, Hesperitin’s modulatory role in altering such circuits has never been
investigated in mBC.

Objective:
The aim of this study is to investigate the impact of hesperitin on miR-486-5p/H19/ICAM-1 axis.

Methodology:
BC patients (n=20) were recruited in the study. Bioinformatic analysis was performed using different
prediction softwares. MDA-MB-231 and MCF-7 cells were cultured and transfected using several
oligonucleotides or treated with serial dilutions of hesperitin. RNA was extracted and gene expression analysis
was performed using q-RT-PCR. ICAM-1 protein levels were assessed using human ICAM-1 Elisa Kit.
Cytotoxic potential of hesperitin against normal cells was assessed by LDH assay. Several functional analysis
experiments were performed such as MTT, colony forming and migration assays.

Results:
The study showed that miR-486-5p and H19 had paradoxical expression profiles in BC patients. miR-
486-5p mimics and H19 siRNAs repressed ICAM-1 and halted mBC hallmarks. A novel crosstalk between miR-
486-5p and H19 was observed highlighting a bi-directional relationship between them. Hesperetin restored the
expression of miR-486-5p, inhibited H19 lncRNA and ICAM-1 expression and selectively regressed mBC cell
aggressiveness.

Conclusion:
miR-486-5p and H19 are inter-connected upstream regulators for ICAM-1 building up miR-486-
5p/H19/ICAM-1 axis that has been successfully tuned in mBC cells by hesperitin.
TSH Variations in Chronic Heart Failure Outpatients: Clinical Correlates and Outcomes
Authors: Terlizzese Paola , Albanese Miriam , Grande Dario , Parisi Giuseppe , Gioia Margherita Ilaria , Brunetti Natale Daniele , Lisi Francesco , Lisco Giuseppe , Di Serio Francesca , Ciccone Marco Matteo , Triggiani Vincenzo , Iacoviello Massimo 
Published: May 2021

Background:
Hypothyroidism is a frequently observed comorbidity in patients with
chronic heart failure (CHF), possibly giving rise to unfavorable outcomes.

Aim:
The aim of the study was to evaluate the impact of TSH changes over time on cardiac function
and prognosis of outpatients with CHF.

Methods:
Patients underwent clinical, electrocardiographic, and echocardiographic evaluations at
baseline and after 12 months. Moreover, blood chemistry tests were performed to evaluate renal
function, cardiac biomarkers, fT3, fT4, and TSH levels. Based on TSH serum levels, patients were
retrospectively classified into four categories: Group 1, patients with improved thyroid function at
one-year follow up vs. baseline; Group 2, patients with stable and mildly high TSH values (3.74 –
10 mUI/L); Group 3, patients with worsening thyroid function; Euthyroid patients Group, TSH levels
within the normal range of reference at baseline as well as at 12 months follow-up. We considered
as end-points: one-year changes of laboratory and echocardiographic parameters; hospitalizations
due to worsening of HF (acute decompensated heart failure - ADHF); death for all causes.

Results:
Among 257 patients, 174 (67.7%) were euthyroid at baseline and after 12 months. Group
1 patients (n. 22, 8.6%) showed a significant improvement in systolic and diastolic function, filling
pressures, NT-proBNP and Galectin-3. Group 2 patients (n. 34, 13.2%) did not exhibit significant
modifications in studied parameters. Group 3 patients (n. 27, 10.5%) showed worsening of diastolic
function and NT-proBNP and a greater risk of ADHF (HR: 2.12; 95%CI: 1.20-3.74; p: 0.009)
and death (HR: 4.05; 95%CI: 2.01-8.15; p<0.001).

Conclusion:
In patients with CHF, changes in thyroid function over time influenced echocardiographic
parameters and biomarkers reflecting modifications of cardiac function and prognosis, thus
suggesting the clinical relevance of thyroid deficiency screening and correction.
Characteristics of the Iron-responsive Element (IRE) Stems in the Untranslated Regions of Animal mRNAs
Authors: Wang Bin , Thompson Michael S. , Adkins Kevin M. 
Published: May 2021

Background:
Iron-responsive Elements (IREs) are hairpin structures located in the 5’ or 3’ untranslated region of some animal mRNAs. IREs have a highly conserved terminal loop and a UGC/C or C bulge five bases upstream of the terminal loop, which divides the hairpin stem into an upper stem and a lower stem.

Objective:
The objective of this study was to investigate the base-pair composition of the upper and lower stems of IREs to determine whether they are highly conserved among mRNAs from different genes.

Methods:
The mRNA sequences of six 5’IREs and five 3’IREs from several animal species were retrieved from the National Center for Biotechnology Information. The folding free energy of each IRE mRNA sequence was predicted using the RNAfold WebServer.

Results:
We found that the upper and lower stems of IREs are not highly conserved among the mRNAs of different genes. There are no statistically significant differences in the IRE structures or folding free energies between mammalian and non-mammalian species relative to either the ferritin heavy chain 5’IRE or ferroportin 5’IRE. There are no overall significant differences in the folding free energies between UGC/C-containing 5’IREs and C-bulge-containing 5’IREs, or between 5’IREs and 3’IREs.

Conclusion:
Further studies are needed to investigate whether the variations in IRE stem composition are responsible for fine-tuning the IRE/Iron-Regulatory Protein interactions among different mRNAs to maintain the balance of cellular iron metabolism, and to identify whether evolutionary processes drive the base-pair composition of the upper and lower stems of IREs toward any particular configuration.
Perception of Mental Health Care Professionals in Saudi Arabia on Computerized Cognitive Behavioral Therapy: Observational Cross-sectional Study
Authors: AlHadi Ahmad N , Alammari Khawla A , Alsiwat Lojain J , Alhaidri Nojood E , Alabdulkarim Nouf H , Altwaijri Nouf A , AlSohaili Shamma A 
Published: May 2021

Background
Mental health disorders are common in Saudi Arabia with a 34% lifetime prevalence. Cognitive behavioral therapy (CBT), a type of psychotherapy, is an evidence-based intervention for the majority of mental disorders. Although the demand for CBT is increasing, unfortunately, there are few therapists available to meet this demand and the therapy is expensive. Computerized cognitive behavioral therapy (cCBT) is a new modality that can help fill this gap.

Objective
We aimed to measure the knowledge of cCBT among mental health care professionals in Saudi Arabia, and to evaluate their attitudes and preferences toward cCBT.

Methods
This quantitative observational cross-sectional study used a convenience sample, selecting mental health care professionals working in the tertiary hospitals of Saudi Arabia. The participants received a self-administered electronic questionnaire through data collectors measuring their demographics, knowledge, and attitudes about cCBT, and their beliefs about the efficacy of using computers in therapy.

Results
Among the 121 participating mental health care professionals, the mean age was 36.55 years and 60.3% were women. Most of the participants expressed uncertainty and demonstrated a lack of knowledge regarding cCBT. However, the majority of participants indicated a positive attitude toward using computers in therapy. Participants agreed with the principles of cCBT, believed in its efficacy, and were generally confident in using computers. Among the notable results, participants having a clinical license and with cCBT experience had more knowledge of cCBT. The overall attitude toward cCBT was not affected by demographic or work-related factors.

Conclusions
Mental health care professionals in Saudi Arabia need more education and training regarding cCBT; however, their attitude toward its use and their comfort in using computers in general show great promise. Further research is needed to assess the acceptance of cCBT by patients in Saudi Arabia, in addition to clinical trials measuring its effectiveness in the Saudi population.
Surgical Treatments for Legg-Calvé-Perthes Disease: Comprehensive Review
Authors: Maleki Arash , Qoreishy Seyyed Mohammad , Bahrami Mohammad Nabi 
Published: May 2021

BackgroundLegg-Calvé-Perthes disease (LCPD) is a common public health problem that usually occurs between the ages of 4 and 8 years, but it can occur between the ages of 2 and 15 years. This condition occurs due to the interruption of blood supply to the femoral head. Up to now, different surgical and nonsurgical treatments, including femoral varus osteotomy, innominate osteotomy, pelvic osteotomies, triple osteotomy, Chiari osteotomy, and shelf acetabuloplasty, have been suggested for noncontainable LCPD hips.ObjectiveThe aim of this comprehensive review was to investigate the various surgical techniques used for LCPD.MethodsAn advanced electronic search of the English-language literature was performed from October 8 to 14, 2020. The electronic databases PubMed, MEDLINE, Web of Science, Embase, Ovid, and Google scholar were searched using appropriate search terms. A manual search of references also was performed. After retrieving the studies, duplicates were removed, and the remining studies were screened based on the title, abstract, and full text. The quality of the selected articles was assessed, and the required data were extracted from eligible articles.ResultsA total of 22 studies were included in the review. Based on the results of the reviewed studies, there are three main factors that influence the treatment outcomes in patients with Perthes disease. These factors are onset age, femoral head involvement severity, and treatment method. The disease has a poor prognosis in children over 8 years old, but this group of patients can also benefit from advanced surgical methods. In patients aged less than 6 years, the disease has a generally good prognosis, but in those aged between 6 and 8 years, its prognosis is variable. Thus, the need for surgical intervention requires close observation of signs. Once any head signs are observed, dynamic arthrography is beneficial before choosing the treatment approach.ConclusionsThis review provides clinicians with a brief guideline for the treatment of patients with LCPD.
Pharmacist-Led Mobile Health Intervention and Transplant Medication Safety
Authors: Gonzales Haley M. , Fleming James N. , Gebregziabher Mulugeta , Posadas-Salas Maria Aurora , Su Zemin , McGillicuddy John W. , Taber David J. 
Published: April 2021

Background and objectives
Medication safety events are predominant contributors to suboptimal graft outcomes in kidney transplant recipients. The goal of this study was to examine the efficacy of improving medication safety through a pharmacist-led, mobile health–based intervention.

Design, setting, participants, & measurements
This was a 12-month, single-center, prospective, parallel, two-arm, single-blind, randomized controlled trial. Adult kidney recipients 6–36 months post-transplant were eligible. Participants randomized to intervention received supplemental clinical pharmacist–led medication therapy monitoring and management via a mobile health–based application, integrated with risk-guided televisits and home-based BP and glucose monitoring. The application provided an accurate medication regimen, timely reminders, and side effect surveys. Both the control and intervention arms received usual care, including serial laboratory monitoring and regular clinic visits. The coprimary outcomes were to assess the incidence and severity of medication errors and adverse events.

Results
In total, 136 kidney transplant recipients were included, 68 in each arm. The mean age was 51 years, 57% were male, and 64% were Black individuals. Participants receiving the intervention experienced a significant reduction in medication errors (61% reduction in the risk rate; incident risk ratio, 0.39; 95% confidence interval, 0.28 to 0.55; P<0.001) and a significantly lower incidence risk of Grade 3 or higher adverse events (incident risk ratio, 0.55, 95% confidence interval, 0.30 to 0.99; P=0.05). For the secondary outcome of hospitalizations, the intervention arm demonstrated significantly lower rates of hospitalizations (incident risk ratio, 0.46; 95% confidence interval, 0.27 to 0.77; P=0.005).

Conclusions
We demonstrated a significant reduction in medication errors, adverse events, and hospitalizations using a pharmacist-led, mobile health–based intervention.
Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits
Authors: S. Sujitha  , P. Venkatalakshmi  
Published: May 2021

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.
Impact of Azithromycin and 8-Hydroxychloroquine in 2019 Novel Coronavirus (COVID-19) Pandemic: A Systematic Review
Authors: Srikanta Chandra  , Preeta Bose  , Jyoti Saxena  , Muniraj Bhattacharya  , Arun dutta  , Jyotirmay Samanta  , Lucky Mukherjee  
Published: May 2021

As we know novel coronavirus is an emergent nuisance in this stipulated period. Corona virus is a group of enveloped viruses, with non-segmented, single stranded & positive sense RNA genomes. Human Corona virus is mainly subdivided into four categories such as 229E, NL63, OC43, HKU1. Epidemiologically it has a greater prevalence in the modern era. The features encountered in the clinical course of the disease are multifarious spanning from cough, sneezing, fever, breathlessness. It may take 2-14 days for a person to notice symptoms after infection. Azithromycin and 8 Hydroxychloroquine both plays an instrumental role for management of COVID-19. Azithromycin is a macrolide antibiotic and it binds with a 50s ribosome then inhibits bacterial protein synthesis. On the other hand 8-Hydroxychloroquine was approved by United State in the year of 1955 .Basically it is used as a antimalarial drugs . Briefly, in inflammatory conditions it binds with toll like receptor & blocks them. 8- hydroxychloroquine increases lysosomal pH in antigen presenting cells . In inflammatory conditions it blocks toll like receptors on plasmacytoid dendritic cells. In our review we focused on the role of Azithromycin, and 8-hydroxychloroquine in Covid-19 .
Pemanfaatan Trichokompos Pada Budidaya Sayuran Organik Sebagai Kegiatan Entrepreneurship Himae
Authors: Jasminarni Jasminarni , Lestari Ardiyaningsih Puji , Evita Evita , Novita Trias 
Published: May 2021

Kegiatan ini bertujuan meningkatkan pengetahuan dan ketrampilan mitra melalui pendampingan, penyuluhan dan demontrasi tentang pemanfaatan trichokompos pada budidaya sayuran organik sebagai kegiatan Entrepreneurship HIMAE (Himpunan Mahasiswa Agroekoteknologi). Secara umum target luaran yang akan dicapai akan memberi dampak terhadap Masyarakat umumnya dan kelompok mitra khususnya, serta masyarakat disekitarnya. Dampak positif yang diharapkan tercapai melalui kegiatan ini dimana tim pengabdiaan akan melakukan pelatihan dan demontrasi kelompok HIMAE sebagai mitra dalam hal pemanfaatan trichokompos yang merupakan hasil produk pada kegiatan PPM tahun sebelumnya sebagai pupuk dan juga dapat berdampak terhadap lingkungan yang bersih dan sehat. Tim pengabdian juga akan memberikan cara cara untuk meningkatkan nilai jual produk sayuran organik dengan cara membuat kemasan. Diharapkan dari upaya tersebut produk sayuran organik yang dihasilkan dapat dijadikan sebagai kegiatan wirausaha yang bernilai ekonomi bagi HIMAE, sehingga dapat menjadi tambahan penghasilan bagi mitra. Luaran yang dihasikan adalah berupa produk sayuran organik. Pemanfaatan trichokompos pada budidaya sayuran merupakan salah satu upaya membantu mengatasi pencemaran lingkungan dan kerusakan lahan akibat penggunaan pupuk kimia. Hasil yang didapat dari kegiatan ini adalah meningkatnya pengetahuan mitra tentang pentingnya penggunaan pupuk organik pada budidaya sayuran organik yang dihasilkan. Sayuran organik yang dapat di kosumsi dan di jual sebagai kegiatan Entrepreneurship HIMAE
Factors affecting repositioning policy compliance: an integrative review
Authors: Iblasi Abdulkareem , Aungsuroch Yupin , Gunwan Joko 
Published: April 2021

Abstract

Objective
To explore the factors affecting nurses’ compliance with repositioning policy.

Methods
An integrative review was conducted following the Whittemore and Knafl methodology to identify the problem related to repositioning policy compliance. We searched the following databases: Coherence Wounds Group Specialized Register (Jan 1997 to Jun 2019), Ovid MEDLINE (Jan 1997 to Jun 2019), EBSCO CINAHL (Jan 1997 to Jun 2019), and Clinical Key database (Jan 2014 to Oct 2018).

Results
The review revealed three factors that influence repositioning compliance: nurse-related factors, patient-related factors, and Environment-related factors.

Conclusions
These factors directly impact one another and, in turn, influence the compliance of nurses to the repositioning policy. However, there is no evidence currently available that explains the collective impact of these factors and how they interact to affect repositioning policy compliance. Nevertheless, all these factors are important and should be considered to enhance and further improve the quality of nursing care and adherence to the repositioning policy.
Tanggap Pertumbuhan dan Perkembangan Jagung Ketan terhadap Pemberian Amelioran dan Pupuk NPK pada Tanah Ultisol
Authors: Harini Dini , Radian  , Iwan Sasli  
Published: April 2021

Pengaplikasian jenis bahan organik sebagai amelioran dan pemupukan NPK dapat menambah kesuburan tanah ultisol melalui sifat fisik dan kimia. Tujuan penelitian untuk mempelajari pengaruh jenis amelioran, dosis pupuk NPK serta interaksinya terhadap pertumbuhan dan perkembangan jagung ketan pada tanah ultisol. Pelaksanaan percobaan di Kabupaten Bengkayang, Kalimantan Barat, pada bulan Maret sampai Agustus 2020. Percobaan disusun menggunakan rancangan acak kelompok faktorial. Faktor pertama pemberian jenis amelioran dengan 5 taraf yaitu biocar bonggol jagung, bokasi berangkas jagung, bokasi jerami padi, pupuk kandang sapi, pupuk kandang ayam. Faktor kedua pemberian dosis pupuk NPK dengan 4 taraf yaitu 100, 200, 300, dan 400 kg ha-1. Jenis amelioran pupuk kandang ayam dapat menambah tinggi tanaman 2, 4, dan 6 MST, diameter tongkol, panjang tongkol, bobot per tongkol, dan bobot tongkol per petak. Dosis pupuk NPK yang tebaik untuk produksi jagung ketan yaitu 200 kg ha-1 pada panjang tongkol dan bobot per tongkol, dan dosis 100 kg ha-1 dapat memacu pertumbuhan tanaman yaitu pada tinggi tanaman 4 MST. Kombinasi jenis amelioran dari pupuk kandang ayam dan pupuk NPK 400 kg ha-1 menghasilkan bobot kering akar, bobot kering tajuk,dan laju pertumbuhan relatif yang tertinggi.
Kata kunci: bahan organik, kesuburan tanah, produksi tanaman, pupuk anorganik