bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs
Authors: Smolinska Agnieszka , Jessop David S. , Pappan Kirk L. , De Saedeleer Alexandra , Kang Amerjit , Martin Alexandra L. , Allsworth Max , Tyson Charlotte , Bos Martine P. , Clancy Matt , Morel Mike , Cooke Tony , Dymond Tom , Harris Claire , Galloway Jacqui , Bresser Paul , Dijkstra Nynke , Jagesar Viresh , Savelkoul Paul H. M. , Beuken Erik V. H. , Nix Wesley H. V. , Louis Renaud , Delvaux Muriel , Calmes Doriane , Ernst Benoit , Pollini Simona , Peired Anna , Guiot Julien , Tomassetti Sara , Budding Andries E. , McCaughan Frank , Marciniak Stefan J. , van der Schee Marc P. 
Published: June 2021

AbstractFace masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whether filters are suited to capture viral particles for SARS-CoV-2 detection. In this study, we generated inactivated virus-laden aerosols of 0.3–2 microns in diameter (0.9 µm mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100 copies/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. The ability to detect nucleic acids on face mask filters may, however, find other uses worth future investigation.
The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
Authors: Oliveira Cleide , Valois Mayara Vioto , Ottaiano Tatiana Fontes , Miranda Antonio , Hansen Daiane , Sampaio Misako Uemura , Oliva Maria Luiza Vilela , de Abreu Maffei Francisco Humberto 
Published: June 2021

AbstractThe anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His(6) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His(6), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His(6) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His(6) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His(6) (1.40 mg/kg), with the rBbCI-His(6) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His(6) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His(6) alone had an antithrombotic effect, not altering blood clotting or bleeding time.
The risk of major bleeding event in patients with chronic kidney disease on pentoxifylline treatment
Authors: Fang Jing-Hung , Chen Yi-Chen , Ho Chung-Han , Chen Jui-Yi , Hsing Chung-Hsi , Liang Fu-Wen , Wu Chia-Chun 
Published: June 2021

AbstractPatients with chronic kidney diseases (CKD) are often treated with antiplatelets due to aberrant haemostasis. This study aimed to evaluate the bleeding risk with CKD patients undergoing pentoxifylline (PTX) treatment with/without aspirin. In this retrospective study, we used Taiwan’s National Health Insurance Research Database to identify PTX treated CKD patients. Patients undergoing PTX treatment after CKD diagnosis were PTX group. A 1:4 age, sex and aspirin used condition matched CKD patients non-using PTX were identified as controls. The outcome was major bleeding event (MBE: intracranial haemorrhage (ICH) and gastrointestinal tract bleeding) during 2-year follow-up period. Risk factors were estimated using Cox regression for overall and stratified analysis. The PTX group had higher MBE risk than controls (hazard ratio (HR) 1.19; 95% confidence interval (CI) 0.94–1.50). In stratified analysis, hyperlipidaemia was a significant risk factor (HR: 1.42; 95% CI 1.01–2.01) of MBE. A daily PTX dose larger than 800 mg, females, non-regular aspirin usage, and ischaemic stroke were risk factors for MBE in PTX group. When prescribing PTX in CKD patients, bleeding should be closely monitored, especially in those with daily dose more than 800 mg, aspirin users, and with a history of ischaemic stroke.
Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2
Authors: Yenamandra Surya Pavan , Koo Carmen , Chiang Suzanna , Lim Han Shi Jeri , Yeo Zhen Yuan , Ng Lee Ching , Hapuarachchi Hapuarachchige Chanditha 
Published: June 2021

AbstractDengue virus type 2 (DENV-2) contributes substantially to the dengue burden and dengue-related mortality in the tropics and sub-tropics. DENV-2 includes six genotypes, among which cosmopolitan genotype is the most widespread. The present study investigated the evolution, intra-genotype heterogeneity and dispersal of cosmopolitan genotype to understand unique genetic characteristics that have shaped the molecular epidemiology and distribution of cosmopolitan lineages. The spatial analysis demonstrated a wide geo-distribution of cosmopolitan genotype through an extensive inter-continental network, anchored in Southeast Asia and Indian sub-continent. Intra-genotype analyses using 3367 envelope gene sequences revealed six distinct lineages within the cosmopolitan genotype, namely the Indian sub-continent lineage and five other lineages. Indian sub-continent lineage was the most diverged among six lineages and has almost reached the nucleotide divergence threshold of 6% within E gene to qualify as a separate genotype. Genome wide amino acid signatures and selection pressure analyses further suggested differences in evolutionary characteristics between the Indian sub-continent lineage and other lineages. The present study narrates a comprehensive genomic analysis of cosmopolitan genotype and presents notable genetic characteristics that occurred during its evolution and global expansion. Whether those characteristics conferred a fitness advantage to cosmopolitan genotype in different geographies warrant further investigations.
Identification of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA
Authors: Ma Yunfei , Yoshida Takeshi , Matoba Kazutaka , Kida Katsuhiko , Shintani Rito , Piao Yingshi , Jin Jingchun , Nishino Taito , Hanayama Rikinari 
Published: June 2021

AbstractExtracellular vesicles (EVs) are secreted from most cells and play important roles in cell–cell communication by transporting proteins, lipids, and nucleic acids. As the involvement of EVs in diseases has become apparent, druggable regulators of EV secretion are required. However, the lack of a highly sensitive EV detection system has made the development of EV regulators difficult. We developed an ELISA system using a high-affinity phosphatidylserine-binder TIM4 to capture EVs and screened a 1567-compound library. Consequently, we identified one inhibitor and three activators of EV secretion in a variety of cells. The inhibitor, apoptosis activator 2, suppressed EV secretion via a different mechanism and had a broader cellular specificity than GW4869. Moreover, the three activators, namely cucurbitacin B, gossypol, and obatoclax, had broad cellular specificity, including HEK293T cells and human mesenchymal stem cells (hMSCs). In vitro bioactivity assays revealed that some regulators control EV secretion from glioblastoma and hMSCs, which induces angiogenesis and protects cardiomyocytes against apoptosis, respectively. In conclusion, we developed a high-throughput method to detect EVs with high sensitivity and versatility, and identified four compounds that can regulate the bioactivity of EVs.
Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis
Authors: Nakamura Ryosuke , Hiwatashi Nao , Bing Renjie , Doyle Carina P. , Branski Ryan C. 
Published: June 2021

AbstractVocal fold (VF) fibrosis is a major cause of intractable voice-related disability and reduced quality of life. Excision of fibrotic regions is suboptimal and associated with scar recurrence and/or further iatrogenic damage. Non-surgical interventions are limited, putatively related to limited insight regarding biochemical events underlying fibrosis, and downstream, the lack of therapeutic targets. YAP/TAZ integrates diverse cell signaling events and interacts with signaling pathways related to fibrosis, including the TGF-β/SMAD pathway. We investigated the expression of YAP/TAZ following vocal fold injury in vivo as well as the effects of TGF-β1 on YAP/TAZ activity in human vocal fold fibroblasts, fibroblast-myofibroblast transition, and TGF-β/SMAD signaling. Iatrogenic injury increased nuclear localization of YAP and TAZ in fibrotic rat vocal folds. In vitro, TGF-β1 activated YAP and TAZ in human VF fibroblasts, and inhibition of YAP/TAZ reversed TGF-β1-stimulated fibroplastic gene upregulation. Additionally, TGF-β1 induced localization of YAP and TAZ in close proximity to SMAD2/3, and nuclear accumulation of SMAD2/3 was inhibited by a YAP/TAZ inhibitor. Collectively, YAP and TAZ were synergistically activated with the TGF-β/SMAD pathway, and likely essential for the fibroplastic phenotypic shift in VF fibroblasts. Based on these data, YAP/TAZ may evolve as an attractive therapeutic target for VF fibrosis.
Identifying aphid resistance in the ancestral wheat Triticum monococcum under field conditions
Authors: Simon Amma L. , Caulfield John C. , Hammond-Kosack Kim E. , Field Linda M. , Aradottir Gudbjorg I. 
Published: June 2021

AbstractWheat is an economically, socially, and nutritionally important crop, however, aphid infestation can often reduce wheat yield through feeding and virus transmission. Through field phenotyping, we investigated aphid resistance in ancestral wheatTriticum monococcum(L.). Aphid (Rhopalosiphum padi(L.), Sitobion avenae(F.) andMetopolophium dirhodum(Wlk.)) populations and natural enemy presence (parasitised mummified aphids, ladybird adults and larvae and lacewing eggs and larvae) on two naturally susceptible wheat varieties,Triticum aestivum(L.) var. Solstice andT. monococcumMDR037, and three potentially resistant genotypesT. monococcumMDR657, MDR045 and MDR049 were monitored across three years of field trials.Triticum monococcumMDR045 and MDR049 had smaller aphid populations, whereas MDR657 showed no resistance. Overall, natural enemy presence was positively correlated with aphid populations; however, MDR049 had similar natural enemy presence to MDR037 which is susceptible to aphid infestation. It is hypothesised that alongside reducing aphid population growth, MDR049 also confers indirect resistance by attracting natural enemies. The observed resistance to aphids in MDR045 and MDR049 has strong potential for introgression into commercial wheat varieties, which could have an important role in Integrated Pest Management strategies to reduce aphid populations and virus transmission.
The reliability and validity of DSM 5 diagnostic criteria for neurocognitive disorder and relationship with plasma neurofilament light in a down syndrome population
Authors: Pape Sarah E. , al Janabi Tamara , Ashton Nicholas J. , Hye Abdul , Sheehan Rory , Gallagher Paul , Knight Bernice , Prins Anne-Marije , Courtenay Ken , Jordanova Vesna , Thomas Bini , Perumal Nagarajan , Forbes Craig , Hassiotis Angela , Strydom Andre 
Published: June 2021

AbstractThe validity of dementia diagnostic criteria depends on their ability to distinguish dementia symptoms from pre-existing cognitive impairments. The study aimed to assess inter-rater reliability and concurrent validity of DSM-5 criteria for neurocognitive disorder in Down syndrome. The utility of mild neurocognitive disorder as a distinct diagnostic category, and the association between clinical symptoms and neurodegenerative changes represented by the plasma biomarker neurofilament light were also examined. 165 adults with Down syndrome were included. Two clinicians independently applied clinical judgement, DSM-IV, ICD-10 and DSM-5 criteria for dementia (or neurocognitive disorder) to each case. Inter-rater reliability and concurrent validity were analysed using the kappa statistic. Plasma neurofilament light concentrations were measured for 55 participants as a marker of neurodegeneration and between group comparisons calculated. All diagnostic criteria showed good inter-rater reliability apart from mild neurocognitive disorder which was moderate (k = 0.494). DSM- 5 criteria had substantial concurrence with clinical judgement (k = 0.855). When compared to the no neurocognitive disorder group, average neurofilament light concentrations were higher in both the mild and major neurocognitive disorder groups. DSM-5 neurocognitive disorder criteria can be used reliably in a Down syndrome population and has higher concurrence with clinical judgement than the older DSM-IV and ICD-10 criteria. Whilst the inter-rater reliability of the mild neurocognitive disorder criteria was modest, it does appear to identify people in an early stage of dementia with underlying neurodegenerative changes, represented by higher average NfL levels.
Uncovering complexity details in actigraphy patterns to differentiate the depressed from the non-depressed
Authors: George Sandip Varkey , Kunkels Yoram K , Booij Sanne , Wichers Marieke 
Published: June 2021

AbstractWhile the negative association between physical activity and depression has been well established, it is unclear what precise characteristics of physical activity patterns explain this association. Complexity measures may identify previously unexplored aspects of objectively measured activity patterns, such as the extent to which individuals show repetitive periods of physical activity and the diversity in durations of such repetitive activity patterns. We compared the complexity levels of actigraphy data gathered over 4 weeks ($$\sim 40000$$

∼
40000

data points each) for every individual, from non-depressed ($$n=25$$

n
=
25

) and depressed ($$n=21$$

n
=
21

) groups using recurrence plots. Significantly lower levels of complexity were detected in the actigraphy data from the depressed group as compared to non-depressed controls, both in terms of lower mean durations of periods of recurrent physical activity and less diversity in the duration of these periods. Further, diagnosis of depression was not significantly associated with mean activity levels or measures of circadian rhythm stability, and predicted depression status better than these.
SpO2/FiO2 as a predictor of high flow nasal cannula outcomes in children with acute hypoxemic respiratory failure
Authors: Kim Ga Eun , Choi Sun Ha , Park Mireu , Jung Jae Hwa , Lee Myeongjee , Kim Soo Yeon , Kim Min Jung , Kim Yoon Hee , Kim Kyung Won , Sohn Myung Hyun 
Published: June 2021

AbstractThe high-flow nasal cannula (HFNC) is a useful treatment modality for acute hypoxemic respiratory failure (AHRF) in children. We compared the ability of the oxygen saturation to fraction of inspired oxygen ratio (S/F) and arterial oxygen partial pressure to fraction of inspired oxygen ratio (P/F) to predict HFNC outcomes in children with AHRF. This study included children treated with HFNC due to AHRF from April 2013 to March 2019 at the Severance Children’s Hospital. HFNC failure was defined as the need for mechanical ventilation. Trends of S/F and P/F during HFNC were analyzed. To predict HFNC outcomes, a nomogram was constructed based on predictive factors. A total of 139 patients with arterial blood gas data were included in the S/F and P/F analyses. S/F < 230 at initiation showed high prediction accuracy for HFNC failure (area under the receiver operating characteristic curve: 0.751). Univariate analyses identified S/F < 230 at HFNC initiation and < 200 at 2 h (odds ratio [OR] 12.83, 95% CI 5.06–35.84), and hemato-oncologic disease (OR 3.79, 95% CI 1.12–12.78) as significant predictive factors of HFNC failure. The constructed nomogram had a highly predictive performance, with a concordance index of 0.765 and 0.831 for the exploratory and validation groups, respectively. S/F may be used as a predictor of HFNC outcomes. Our nomogram with S/F for HFNC failure within 2 h may prevent delayed intubation in children with AHRF.
Hangboard training in advanced climbers: A randomized controlled trial
Authors: Mundry Saskia , Steinmetz Gino , Atkinson Elizabeth J. , Schilling Arndt F. , Schöffl Volker R. , Saul Dominik 
Published: June 2021

AbstractImproving climbing performance strongly depends upon effective training methods. Hangboard training is one of the most popular approaches to increase finger and forearm strength. Training protocols are based on maximizing weight or minimizing edges. We aimed to evaluate which of these protocols was superior. We prospectively analyzed 30 intermediate to advanced climbing athletes [Union Internationale des Associations d'Alpinisme (UIAA) VI–VIII] and randomized them into three groups: control group C (Control, normal climbing training), hangboard group HE (Hang endurance, grips to hold for a determined time decreased every week), and hangboard group HW (Hang weight, + 1.25 kg weight were added each week to hold for a determined time). As endpoints, we measured the grip strength before and after an 8-week training protocol in seven different pinches. Over the 8-week training period, HW hangboard training significantly improved the climbers’ grip strength compared to C [p = 0.032, effect size (ES) 0.36]. Maximizing weight improved the strength in I/II + III, I/II + III + IV and fist significantly. HW was superior compared to C in terms of grip strength improvement in three out of seven pinches and overall grip strength. The overall changes in the HE group did not differ significantly from the C group. An 8-week training protocol with increasing weights (HW) significantly improved overall grip strength more than a regular climbing training without the use of a hangboard.
Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
Authors: Iqbal Muhammad Arsalan , Ali Asghar , Hadlich Frieder , Oster Michael , Reyer Henry , Trakooljul Nares , Sommerfeld Vera , Rodehutscord Markus , Wimmers Klaus , Ponsuksili Siriluck 
Published: June 2021

AbstractPhosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (n = 80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.
Unraveling regulatory divergence, heterotic malleability, and allelic imbalance switching in rice due to drought stress
Authors: Ereful Nelzo C. , Laurena Antonio , Liu Li-Yu , Kao Shu-Min , Tsai Eric , Greenland Andy , Powell Wayne , Mackay Ian , Leung Hei 
Published: June 2021

AbstractThe indica ecotypes, IR64, an elite drought-susceptible variety adapted to irrigated ecosystem, and Apo (IR55423-01 or NSIC RC9), a moderate drought-tolerant upland genotype together with their hybrid (IR64 × Apo) were exposed to non- and water-stress conditions. By sequencing (RNA-seq) these genotypes, we were able to map genes diverging in cis and/or trans factors. Under non-stress condition, cis dominantly explains (11.2%) regulatory differences, followed by trans (8.9%). Further analysis showed that water-limiting condition largely affects trans and cis + trans factors. On the molecular level, cis and/or trans regulatory divergence explains their genotypic differences and differential drought response. Between the two parental genotypes, Apo appears to exhibit more photosynthetic efficiency even under water-limiting condition and is ascribed to trans. Statistical analyses showed that regulatory divergence is significantly influenced by environmental conditions. Likewise, the mode of parental expression inheritance which drives heterosis (HET) is significantly affected by environmental conditions indicating the malleability of heterosis to external factors. Further analysis revealed that the HET class, dominance, was significantly enriched under water-stress condition. We also identified allelic imbalance switching in which several genes prefer IR64- (or Apo-) specific allele under non-stress condition but switched to Apo- (or IR64-) specific allele when exposed to water-stress condition.
Impact of proton therapy on antitumor immune response
Authors: Mirjolet Céline , Nicol Anaïs , Limagne Emeric , Mura Carole , Richard Corentin , Morgand Véronique , Rousseau Marc , Boidot Romain , Ghiringhelli François , Noel Georges , Burckel Hélène 
Published: June 2021

AbstractRadiotherapy delivered using photons induces an immune response that leads to modulation of the tumor microenvironment. Clinical studies are ongoing to evaluate immune checkpoint inhibitors in association with photon radiotherapy. At present, there is no publication on the radio-induced immune response after proton therapy. Balb/c mice bearing subcutaneous CT26 colon tumors were irradiated by a single fraction of 16.4 Gy using a proton beam extracted from a TR24 cyclotron. RNA sequencing analysis was assessed at 3 days post-treatment. Proton therapy immune response was monitored by flow cytometry using several panels (lymphoid, myeloid cells, lymphoid cytokines) at 7 and 14 days post-irradiation. RNA-Seq functional profiling identified a large number of GO categories linked to “immune response” and “interferon signaling”. Immunomonitoring evaluation showed induced tumor infiltration by immune cells. This is the first study showing the effect of proton therapy on immune response. These interesting results provide a sound basis to assess the efficacy of a combination of proton therapy and immune checkpoint inhibitors.