bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of uterine leiomyosarcoma-associated hub genes and immune cell infiltration pattern using weighted co-expression network analysis and CIBERSORT algorithm
Authors: Shen Xiaoqing , Yang Zhujuan , Feng Songwei , Li Yi 
Published: July 2021

Abstract
Background
While large-scale genomic analyses symbolize a precious attempt to decipher the molecular foundation of uterine leiomyosarcoma (ULMS), bioinformatics results associated with the occurrence of ULMS based totally on WGCNA and CIBERSORT have not yet been reported. This study aimed to screen the hub genes and the immune cell infiltration pattern in ULMS by bioinformatics methods.

Methods
Firstly, the GSE67463 dataset, including 25 ULMS tissues and 29 normal myometrium (NL) tissues, was downloaded from the public database. The differentially expressed genes (DEGs) were screened by the ‘limma’ package and hub modules were identified by weighted gene co-expression network analysis (WGCNA). Subsequently, gene function annotations were performed to investigate the biological role of the genes from the intersection of two groups (hub module and DEGs). The above genes were calculated in the protein–protein interaction (PPI) network to select the hub genes further. The hub genes were validated using external data (GSE764 and GSE68295). In addition, the differential immune cell infiltration between UL and ULMS tissues was investigated using the CIBERSORT algorithm. Finally, we used western blot to preliminarily detect the hub genes in cell lines.

Results
WGCNA analysis revealed a green-yellow module possessed the highest correlation with ULMS, including 1063 genes. A total of 172 DEGs were selected by thresholds set in the ‘limma’ package. The above two groups of genes were intersected to obtain 72 genes for functional annotation analysis. Interestingly, it indicated that 72 genes were mainly involved in immune processes and the Neddylation pathway. We found a higher infiltration of five types of cells (memory B cells, M0-type macrophages, mast cells activated, M1-type macrophages, and T cells follicular helper) in ULMS tissues than NL tissues, while the infiltration of two types of cells (NK cells activated and mast cells resting) was lower than in NL tissues. In addition, a total of five genes (KDR, CCL21, SELP, DPT, and DCN) were identified as the hub genes. Internal and external validation demonstrated that the five genes were over-expressed in NL tissues compared with USML tissues. Finally, the correlation analysis results indicate that NK cells activated and mast cells activated positively correlated with the hub genes. However, M1-type macrophages had a negative correlation with the hub genes. Moreover, only the DCN may be associated with the Neddylation pathway.

Conclusion
A series of evidence confirm that the five hub genes and the infiltration of seven types of immune cells are related to USML occurrence. These hub genes may affect the occurrence of USML through immune-related and Neddylation pathways, providing molecular evidence for the treatment of USML in the future.
Equity across the cancer care continuum for culturally and linguistically diverse migrants living in Australia: a scoping review
Authors: Scanlon Brighid , Brough Mark , Wyld David , Durham Jo 
Published: July 2021

AbstractInternational evidence suggests migrants experience inequitable access, outcomes and treatment quality across the cancer care continuum. There is currently limited research assessing equity across the cancer care continuum for culturally and linguistically diverse migrants living in Australia. A detailed protocol and search strategy were developed and used to identify all relevant literature, utilising the Joanna Briggs Institute Reviewer’s Manual. Systematic searching was conducted via multiple databases and identified studies were screened against pre-identified inclusion and exclusion criteria. 71 studies met the inclusion criteria for analysis. Most studies examined cancer detection via screening. Very few studies examined cancer prevention, diagnosis, treatment or palliative care. Most studies focused on patient-sided barriers to care and there was a paucity of information regarding institutional barriers to health. Cancer-related outcomes were seldom examined, and most studies were qualitative or behavioral analysis. Results highlighted significant communication issues spanning the cancer care continuum and a context of inadequate support for both patients and clinicians. There is a demonstrable need to examine equity in access and outcomes for culturally and linguistically diverse cancer populations. This requires the identification of cancer-related disparities and an examination of institutional barriers to care. Through addressing this dearth of information, future research and health policy can support the operationalisation of health equity.
Helpful family climate moderates the relationship between perceived family support of ADHD symptoms and depression: a conditional process model
Authors: Pojanapotha Pichaya , Boonnag Chiraphat , Siritikul Sirinut , Chalanunt Sirikorn , Kuntawong Pimolpun , Wongpakaran Nahathai , Wongpakaran Tinakon 
Published: July 2021

Abstract
Background
Symptoms of attention deficit hyperactivity disorder (ADHD) are commonly comorbid with depression This study aimed to examine the relationship between ADHD symptoms and depression through perceived family support and to explore whether the magnitude of the relationship depended on the type of family climate of medical students.

Methods
This cross-sectional study was conducted among 124 first year medical students in Thailand. Participants completed questionnaires on ADHD symptoms, depression, perceived family support, and 9 types of family climate. The questionnaires included the Adult ADHD Self-Report Scale Screener, Patient Health Questionnaire-9, and revised Thai Multidimensional Scale of Perceived Social Support. Mediational analysis was adopted to examine the mediating role of perceived family support in the relationship between ADHD symptoms and depression, while moderation analysis was applied to examine the extent of the relationship depending on family climate.

Results
The relationship between ADHD symptoms and depression was moderate. Perceived family support partially mediated this relationship after controlling for age and sex. Among the types of family climate, only helpful family climate was a significant moderator of perceived family support and depression. The moderated mediation model increased the variance in depression from 17% by the mediation model to 21%. However, follow-up conditional mediational analysis showed that the indirect effect of ADHD symptoms on depression via perceived family support was not significant and that this effect did not vary linearly as a function of helpful family climate.

Conclusion
The findings of the study revealed that poor family support might be one risk of developing depression in the context of ADHD symptoms. Further study on providing intervention concerning family support among those with ADHD symptoms should be warranted. In addition, a study on helpful family climate in a larger sample size, in other populations, and in a longitudinal fashion for a more robust conclusion is encouraged.
Behavioral intentions of technology teachers to implement an engineering-focused curriculum
Authors: Yu Kuang-Chao , Wu Pai-Hsing , Lin Kuen-Yi , Fan Szu-Chun , Tzeng Sy-Yi , Ku Chih-Jung 
Published: July 2021

Abstract
Background
Teaching engineering at a high school level has been a subject of substantial concern during recent curriculum reforms. Many countries are increasingly including engineering-focused subjects in their technology curriculum guidelines. However, technology teachers face challenges regarding the optimal implementation of an engineering-focused curriculum. It is essential to understand technology teachers’ perceptions of and behavior in classroom practices when teaching an engineering-focused curriculum. To explore the factors influencing the effective implementation of the curriculum, this study aimed to explore the association between technology teachers’ perceptions regarding curriculum guidelines (i.e., perceived ease of use, perceived usefulness, and beliefs) and their behavioral intentions. In addition, this study explored how these perceptions change for teachers who participated in a professional development program (PDP) for teaching an engineering-focused curriculum compared with those who did not participate in the program (NoPDP).

Results
In this study, structural equation modeling was used to investigate factors potentially influencing teachers’ behavioral intentions, including subjective norms and perceived behavioral control. The results showed that technology teachers’ behavioral intentions were influenced by their perceptions of the curriculum's usefulness. Subjective norms and perceived behavioral control were also significant determinants of behavioral intentions. Moreover, the results differed between the two groups. Perceived usefulness had direct and indirect effects on the behavioral intentions of the PDP and NoPDP groups, respectively.

Conclusions
We constructed a model of technology teachers’ behavioral intentions to implement an engineering-focused curriculum and identified factors influencing technology teachers’ behavioral intentions to implement an engineering-focused curriculum. Our conclusions are as follows: (1) the model was adequate for determining the factors influencing technology teachers’ behavioral intentions; (2) in the PDP group, perceived usefulness, subjective norms, and perceived behavioral control were significantly associated with behavioral intentions; and (3) in the NoPDP group, perceived usefulness and perceived ease of use were associated with behavioral intentions and self-reported behavior only via the mediating factor of beliefs. The above influencing factors should be taken into account when planning professional development programs for pre- and in-service teachers, as these programs will have implications regarding the successful implementation of an engineering-focused curriculum.
Improved technique of personalised surgical guides generation for mandibular free flap reconstruction using an open-source tool
Authors: Šimić Luka , Kopačin Vjekoslav , Mumlek Ivan , Butković Josip , Zubčić Vedran 
Published: July 2021

AbstractWith advancements in computer systems, computer graphics and medical imaging technologies, clinicians strive for a personalised approach to patient treatment. Therefore, the production of personalised surgical guides is becoming standard. While proprietary software solutions for mandibular reconstruction planning exist, they are often not available due to their high costs. There are multiple alternative methods available, which utilise open-source technologies and free software, but they use advanced three-dimensional (3D) computer-aided design (CAD) concepts. The goal of this article is to provide end-users (surgeons, radiologists, or radiology technicians) with a tool that offers an intuitive interface and a simple workflow. The tool provides only the necessary methods offering a high degree of automation and abstracting the underlying 3D CAD concepts. This is accomplished by providing an add-on (written in Python) for a free and open-source software package Blender.
Risk factors of laryngeal injuries in extubated critical pediatric patients
Authors: Algebaly HebatAllah Fadel , Mohsen Mona , Naguib Maggie Louis , Bazaraa Hafez , Hazem Noran , Aziz Miriam Magdy 
Published: July 2021

Abstract
Background
The larynx in children is unique compared to adults. This makes the larynx more prone to trauma during intubation. Under sedation and frequent repositioning of the tube are recorded as risk factors for laryngeal injury. We examined the larynx of 40 critically ill children in the first 24 h after extubation to estimate the frequency and analyze the risk factors for laryngeal trauma using the classification system for acute laryngeal injury (CALI).

Results
The post-extubation stridor patients had a higher frequency of diagnosis of inborn errors of metabolism, longer duration of ventilation, longer hospital stay, moderate to severe involvement of glottic and subglottic area, frequent intubation attempts, and more than 60 s to intubate Regression analysis of the risk factors of severity of the injury has shown that development of ventilator-associated pneumonia carried the highest risk (OR 32.111 95% CI 5.660 to 182.176), followed by time elapsed till intubation in seconds (OR 11.836, 95% CI 2.889 to 48.490), number of intubation attempts (OR 10.8, CI 2.433 to 47.847), and development of pneumothorax (OR 10.231, 95% CI 1.12 to 93.3).

Conclusion
The incidence of intubation-related laryngeal trauma in pediatric ICU is high and varies widely from mild, non-symptomatic to moderate, and severe and could be predicted by any of the following: prolonged days of ventilation, pneumothorax, multiple tube changes, or difficult intubation.
Building capacity for implementation—the KT Challenge
Authors: Black Agnes T. , Steinberg Marla , Chisholm Amanda E. , Coldwell Kristi , Hoens Alison M. , Koh Jiak Chin , LeBlanc Allana , Mackay Martha , Salmon Amy , Snow M. Elizabeth 
Published: July 2021

Abstract
Background
The KT Challenge program supports health care professionals to effectively implement evidence-based practices. Unlike other knowledge translation (KT) programs, this program is grounded in capacity building, focuses on health care professionals (HCPs), and uses a multi-component intervention. This study presents the evaluation of the KT Challenge program to assess the impact on uptake, KT capacity, and practice change.

Methods
The evaluation used a mixed-methods retrospective pre-post design involving surveys and review of documents such as teams’ final reports. Online surveys collecting both quantitative and qualitative data were deployed at four time points (after both workshops, 6 months into implementation, and at the end of the 2-year funded projects) to measure KT capacity (knowledge, skills, and confidence) and impact on practice change. Qualitative data was analyzed using a general inductive approach and quantitative data was analyzed using non-parametric statistics.

Results
Participants reported statistically significant increases in knowledge and confidence across both workshops, at the 6-month mark of their projects, and at the end of their projects. In addition, at the 6-month check-in, practitioners reported statistically significant improvements in their ability to implement practice changes. In the first cohort of the program, of the teams who were able to complete their projects, half were able to show demonstrable practice changes.

Conclusions
The KT Challenge was successful in improving the capacity of HCPs to implement evidence-based practice changes and has begun to show demonstrable improvements in a number of practice areas. The program is relevant to a variety of HCPs working in diverse practice settings and is relatively inexpensive to implement. Like all practice improvement programs in health care settings, a number of challenges emerged stemming from the high turnover of staff and the limited capacity of some practitioners to take on anything beyond direct patient care. Efforts to address these challenges have been added to subsequent cohorts of the program and ongoing evaluation will examine if they are successful. The KT Challenge program has continued to garner great interest among practitioners, even in the midst of dealing with the COVID-19 pandemic, and shows promise for organizations looking for better ways to mobilize knowledge to improve patient care and empower staff. This study contributes to the implementation science literature by providing a description and evaluation of a new model for embedding KT practice skills in health care settings.
Imaging in non-cystic fibrosis bronchiectasis and current limitations
Authors: Ledda Roberta Eufrasia , Balbi Maurizio , Milone Francesca , Ciuni Andrea , Silva Mario , Sverzellati Nicola , Milanese Gianluca 
Published: July 2021

Non-cystic fibrosis bronchiectasis represents a heterogenous spectrum of disorders characterised by an abnormal and permanent dilatation of the bronchial tree associated with respiratory symptoms. To date, diagnosis relies on computed tomography (CT) evidence of dilated airways. Nevertheless, definite radiological criteria and standardised CT protocols are still to be defined. Although largely used, current radiological scoring systems have shown substantial drawbacks, mostly failing to correlate morphological abnormalities with clinical and prognostic data. In limited cases, bronchiectasis morphology and distribution, along with associated CT features, enable radiologists to confidently suggest an underlying cause. Quantitative imaging analyses have shown a potential to overcome the limitations of the current radiological criteria, but their application is still limited to a research setting. In the present review, we discuss the role of imaging and its current limitations in non-cystic fibrosis bronchiectasis. The potential of automatic quantitative approaches and artificial intelligence in such a context will be also mentioned.
The effects of the glycaemic control on the severity of the delirium in the advanced phase of Alzheimer’s disease
Authors: Martocchia Antonio , Scarienzi Marta , Prunas Pietro , Bentivegna Enrico , Cacciafesta Mauro , Martelletti Paolo , Sesti Giorgio 
Published: July 2021

Background: Behavioral and psychological symptoms of dementia (BPSD) and delirium are common in advanced phases of Alzheimer’s disease (AD). Methods: Thirty-eight moderate-severe AD patients were enrolled (n=16 affected by type 2 diabetes). Each patient received a comprehensive geriatric assessment (CGA) (including evaluation of BPSD and frailty), and a complete metabolic evaluation (including the measurement of the glycated hemoglobin, HbA1c). Results: Both the hyper- and hypo-glycemic extremes of the glycemic spectrum worsened BPSD, but delirium was more susceptible to hypoglycemic events. The severity of delirium was significantly related to cognitive function (r = -0.585, p<0.001) and frailty (r = +0.440, p<0.05). Conclusions: The measurement of HbA1c was useful for evaluating the risk of delirium in relationship to glycemic control and nutritional status.
Dataset for measuring the conceptual understanding of optics in Rwanda
Authors: Ndihokubwayo Kizito , Ralph Michael , Ndayambaje Irénée , Uwamahoro Jean 
Published: July 2021

This dataset is an accumulation of data collected to test Rwandan physics students’ conceptual understanding of light phenomena and to assess instructional tools for active learning of optics. We collected and analysed data from 251 grade 11 (senior 5) students using our Light Phenomena Conceptual Assessment (LPCA) tool and from 136 grade 10 (senior 4) students using Geometric Optics Conceptual Understanding Test (GOCUT) in 2019. Before collecting data, we designed and validated LPCA and GOCUT, and tested their reliability. Data were collected before and after students learnt about the unit of light. Both day and boarding schools in rural and urban areas were included in our sampling. Data collected were test scores from students after performing a 30-item LPCA test or 25-item GOCUT test in 40 minutes. The data may be reused to extend students' understanding of optics concepts through item analysis, analysis of school characteristics such as location and school type, or by analysing students' characteristics such as subject combinations.
Correlation between CD44+ cancer stem cell expression and histopathological types of nasopharyngeal carcinoma
Authors: Yusuf Muhtarum , Indra Indriyadevi , Juniati Sri Herawati , Afriani Dewi Yussy 
Published: July 2021

Background: Nasopharyngeal carcinoma (NPC) recurrency rate is still high despite patients receiving complete treatment. The response to treatment may vary depending on the type of histopathology and Epstein-Barr virus, however the mechanism remains unclear. Recent studies have found that there is a relationship between response to treatment and the presence of cancer stem cells (CSCs). CD44+ cancer stem cells may cause cancer cells to be resistant to treatment. Therefore, this cross-sectional study aims to determine the correlation between CD44 + cancer stem cell expression and the histopathological types of NPC. Method: Samples were obtained from NPC biopsies of type I, II, III patients (based on WHO histopathology criteria), who had not received prior treatment. CD44+ expression was examined using immunohistochemistry methods by staining CD44+ monoclonal antibodies. The degree of CD44+ cell membrane expression was based on the immunoreactive score scale or the Remmele index scale. Results: Most histopathological types were WHO type III (21 patients, 50%), followed by type II (18 patients, 42.86%), and type I (3 patients, 7.14%). CD44 + expression on type I showed one patient had moderate positive and two patients had a high-positive expression. In type II, 10 were moderate positive and eight were high-positive. In type III, one patient was low-positive, 11 were moderate positive and nine patients were high-positive. Statistical analysis showed that the CD44+ expression difference between the three histopathology types were not statistically significant. Conclusion: There were no correlations between CD44 + expression and histopathological type of NPC.
Maximum Principles and ABP Estimates to Nonlocal Lane–Emden Systems and Some Consequences
Authors: Leite Edir Junior Ferreira 
Published: July 2021

Abstract
This paper deals with maximum principles depending on the domain and ABP estimates associated to the following Lane–Emden system involving fractional Laplace operators:

{

(

-
Δ

)

s

⁢
u

=

λ
⁢
ρ
⁢

(
x
)

⁢

|
v
|

α
-
1

⁢
v

in
⁢
Ω

,

(

-
Δ

)

t

⁢
v

=

μ
⁢
τ
⁢

(
x
)

⁢

|
u
|

β
-
1

⁢
u

in
⁢
Ω

,

u

=
v
=
0

in
⁢

ℝ
n

∖
Ω

,

\left\{\begin{aligned} \displaystyle(-\Delta)^{s}u&\displaystyle=\lambda\rho(x% )\lvert v\rvert^{\alpha-1}v&&\displaystyle\phantom{}\text{in }\Omega,\\ \displaystyle(-\Delta)^{t}v&\displaystyle=\mu\tau(x)\lvert u\rvert^{\beta-1}u&% &\displaystyle\phantom{}\text{in }\Omega,\\ \displaystyle u&\displaystyle=v=0&&\displaystyle\phantom{}\text{in }\mathbb{R}% ^{n}\setminus\Omega,\end{aligned}\right.

where

s
,
t

∈

(
0
,
1
)

{s,t\in(0,1)}

,

α
,
β

>
0

{\alpha,\beta>0}

satisfy

α
⁢
β

=
1

{\alpha\beta=1}

, Ω is a smooth bounded domain in

ℝ
n

{\mathbb{R}^{n}}

,

n
≥
1

{n\geq 1}

, and ρ and τ are continuous functions on

Ω
¯

{\overline{\Omega}}

and positive in Ω. We establish some maximum principles depending on Ω. In particular, we explicitly characterize the measure of Ω for which the maximum principles corresponding to this problem hold in Ω. For this, we derived an explicit lower estimate of principal eigenvalues in terms of the measure of Ω. Aleksandrov–Bakelman–Pucci (ABP) type estimates for the above systems are also proved. We also show the existence of a viscosity solution for a nonlinear perturbation of the nonhomogeneous counterpart of the above problem with polynomial and exponential growths. As an application of the maximum principles, we measure explicitly how small

|
Ω
|

{\lvert\Omega\rvert}

has to be to ensure the positivity of the obtained solutions.