bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streptomyces Pimoensis sp. nov., Isolated From the Taklimakan Desert in Xinjiang, China
Authors: Zhang Ping , Luo Xiaoxia , Luo Xinrong , Liu Zhanwen , Xia Zhanfeng , Wan Chuanxing , zhang Lili 
Published: December 2021

Abstract
A novel Streptomyces strain, designated TRM 75549T, was separated from a sample of sand in Pimo, Taklimakan desert, Xinjiang, North-West China. Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM75549T within the genus Streptomyces with the highest similarities to Streptomyces flavoviridis NBRC 12772T (98.76%). The whole-genome average nucleotide identity (ANI) value between strain TRM75549T and S. flavoviridis NBRC 12772T is 88.20%. Digital DNA-DNA hybridization (dDDH) value between strain TRM75549T and S. flavoviridis NBRC 12772T is 44.10%. They are well below the recommended 95-96% and 70% cut-off points for designated species respectively. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, recA, rpoB and trpB) and phylogenomic analysis also illustrated that strain TRM75549T should be assigned to the genus Streptomyces. Strain TRM75549T contained MK-9 (H6) and MK-9 (H8) as predominant menaquinones. The diagnostic diamino acid of cell walls was identified as LL-diaminopimelic acid and Meso-diaminopimelic. The whole-cell sugar pattern of strain TRM 75549T consisted of mannose and glucose. The major fatty acids (>5%) were iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:1H, iso-C16:0. The polar lipids were diphosphatidylglycerol, lysophosphatidylglycerol, phosphatidylethanolamine, phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatiylinositol mannosides and unidentified phospholipids. Strain TRM75549T could be differentiated from S. flavoviridis NBRC 12772T, based on physiological and biochemical characteristics. Based on the data from this polyphasic study presented above, strain TRM75549T is represent ative of a novel species of the genus Streptomyces, for which the name Streptomyces pimoensis sp. nov. is proposed. The type strain is TRM75549T (=CCTCC AA 2020054T=LMG 32221T ).
Hidden Magnetic Texture in the Pseudogap Phase of High-Tc $YBa{2}Cu{3}O_{6.6}$
Authors: Bourges Philippe , Bounoua Dalila , Sidis Yvan , Loew Toshinao , Bourdarot Bourdarot , Boehm Martin , Steffens Paul , Mangin-Thro Lucile , Balédent Victor 
Published: December 2021

Abstract
Despite decades of intense researches, the enigmatic pseudo-gap (PG) phase of superconducting cuprates remains an unsolved mystery. In the last 15 years, various symmetry breakings in the PG state have been discovered, spanning an intra-unit cell (IUC) magnetism, preserving the lattice translational (LT) symmetry but breaking time-reversal symmetry and parity, and an additional incipient charge density wave breaking the LT symmetry upon cooling. However, none of these states can (alone) account for the partial gapping of the Fermi surface. Here we report a hidden LT-breaking magnetism which is crucial for elucidating the PG puzzle. Our polarized neutron diffraction measurements reveal magnetic correlations, in two different underdoped YBa2Cu3O6.6 single crystals, that settle at the PG onset temperature with i) a planar propagation wave vector (π, 0) ≡ (0, π), yielding a doubling or quadrupling of the magnetic unit cell and ii) magnetic moments mainly pointing perpendicular to the CuO2 layers. The LT-breaking magnetism is at short range suggesting the formation of clusters of 5-6 unit cells that, together with the previously reported IUC magnetism, yields a hidden magnetic texture of the CuO2 unit cells hosting loop currents.
Landscape Simplification Increases Bactrocera Oleae Abundance In Olive Groves: Populations Dynamics In Different Land Uses.
Authors: Paredes Daniel , Alves João Frederico , Mendes Sara , Costa José Miguel , Alves Joana , Silva António Alves da , Sousa José Paulo 
Published: December 2021

Abstract
Bactrocera oleae is the main pest in olive groves and its management requires a sustainable perspective to reduce the use of chemical products. Landscape context is being considered as an important driver of pest reduction but results on B. oleae show inconsistency to date. Most of landscape-pest control studies focus on the dynamics of the pests within the focal crop, ignoring these dynamics in other land uses. Here we present a study in which we analyze the seasonal population dynamics of the olive pest B. oleae in the most important land uses of a typical olive landscape in Portugal. We found that B. oleae is present in all the land uses and the dynamics are very similar to those in the olive groves. However, the presence of these land uses in the landscape did not display any increase of B.oleae abundance within the olives groves. In contrast, a landscape mainly composed by olive groves increased the abundance of this pest. Importantly, more diverse landscapes surrounding olive groves reduce the abundance of the olive fly. Based on these findings, we can conclude that B. oleae is present in all the land uses of the studied landscape but that this presence does not imply an increase of B. oleae in olive groves. Indeed, other land uses can promote landscape diversification which is a driver of the reduction of B. oleae populations in olive groves. We thus encourage olive stakeholders to increase landscape diversification around their farms by promoting/restoring other crops/habitats.
Effects of Replacement of Wheat Straw with Corn Stover Based TMR on Growth Performance, Behavioural Characteristics, selected Blood Metabolites and Nutrient Digestibility in Beetal Bucks.
Authors: Majeed Faisal , Ahmed Saeed , Rashid Muhammad Afzal , Shahid Muhammad Qamer , Mohsin Imran 
Published: December 2021

Abstract
An experiment was executed to determine the effects of replacing wheat straw with corn stover on growth performance, behavioural characteristics, blood metabolites and nutrient digestibility in Beetal bucks. A total of twenty four Beetal bucks were assigned randomly to one of three treatment groups, having eight animals each, for 15 weeks experimental period. The dietary treatments included conventional (25% wheat straw and 75% concentrate), corn stover 50 (Fifty percent of wheat straw (12.5%) was replaced with corn stover, and corn stover 100 (Wheat straw was completely replaced with corn stover). Dietary replacement of wheat straw with corn stover has resulted in an increased dry matter intake and average daily gain by 10 and 26%, respectively in the bucks. Rumen pH and fecal score, however, remained unaffected by dietary replacement of wheat straw with corn stover in the bucks. The replacement of wheat straw with corn stover has resulted in an increased feeding and rumination time, improved lying time and length and decreased number of bouts in the bucks. Blood glucose, urea, bilirubin and calcium levels were remained unaffected by replacing wheat straw with corn stover. The blood phosphorous level, however, was decreased in bucks fed corn stover based ration. The bucks fed corn stover based ration has resulted in an increased digestibility of nutrients including organic matter, crude protein, neutral and acid detergent fiber compared to those fed wheat straw based total mixed ration.
Analysis of Macular Blood Flow Alterations In Thyroid Associated Ophthalmopathy
Authors: Zhang Xiaohan , Liu Wangyuan , Zhang Zhaode , Dai Jinhui , Zhang Jinfeng , Lin Ling 
Published: December 2021

Abstract
Purpose: To evaluate the alterations in macular superficial retinal vessels density and relation with visual acuity in thyroid associated ophthalmopathy (TAO) patients with different severity levels.Method: This cross-sectional observational study included 70 TAO patients and 70 healthy controls. Only the data from the right eyes were analyzed. TAO patients were divided into 7 subgroups according to the NOSPECS score grading from 0 to 6 with 10 cases in each subgroup. Foveal avascular zone (FAZ), vascular density (VD) and perfusion density (PD) of macular 1mm diameter and 6mm diameter areas were measured by optical coherence tomography angiography (OCTA). Results: In TAO patients, significant increases were found in macular and foveal vascular densities (FVD) and perfusion densities (FPD) while significant decrease was found in FAZ area when comparing with control group (p<0.05). Spearman correlation analysis and multiple linear regression analysis showed that TAO severity grade was negatively correlated with FVD (β=-1.150, p=0.032), FPD (β=-0.024, p=0.042), MVD (β=-0.583, p=0.020) and MPD (β=-0.011, p=0.010). Clinical activity score (CAS) score showed positive correlation with FVD(β=0.794, p=0.035) and FPD(β=0.017, p=0.041). FVD(β=-0.009, p=0.033), MVD(β=-0.034, p<0.001), FPD(β=-0.416, p=0.039) and MPD(β=-2.428, p<0.001) all showed negative correlation with best corrected visual acuity (BCVA).Conclusion: Superficial macular blood flow in TAO patients showed an overall increase comparing with healthy controls and a decrease as TAO disease severity worsen. Superficial macular blood flow density was negatively correlated with BCVA.
Impact of Modelling Approach on the Estimation of Government Intervention Effects in COVID-19
Authors: Liu Lun , Zhang Zhu , Wang Hui , Wang Shenhao , Zhuang Shengsheng 
Published: December 2021

Abstract
The efficacy of government interventions in epidemic has become a hot subject since the onset of COVID-19. There is however much variation in the results quantifying the effects of interventions, which is partly related to the varying modelling approaches employed by existing studies. This paper therefore aims to examine how the choice of modelling approach would affect the estimation results of intervention effects, by experimenting with different modelling approaches on a same data set composed of the 500 most affected U.S. counties. We compare the most frequently used methods from the two classes of modelling approaches, which are Bayesian hierarchical model from the class of computational approach and difference-in-difference from the class of natural experimental approach. We find that computational methods are likely to produce larger estimates of intervention effects due to simultaneous voluntary behavioral changes. In contrast, natural experimental methods are more likely to extract the true effect of interventions. Among different difference-in-difference estimators, the two-way fixed effect estimator seems to be an efficient one. Our work can inform the methodological choice of future research on this topic, as well as more robust re-interpretation of existing works, to facilitate both future epidemic response plans and the science of public health.
Nonpoint Source Pollution (NPSP) Induces Structural and Functional Variation in the Fungal Community of Sediments in the Jialing River, China
Authors: Xu Fei , Zhu Lanping , Wang Jiaying , Xue Yuqin , Liu Kunhe , Zhang Fubin , ZHANG TUO 
Published: December 2021

Abstract
Nonpoint source pollution (NPSP) from human production and life activities causes severe destruction in river basin environments. In this study, three types of sediment samples (A, NPSP tributary samples; B, non-NPSP mainstream samples; C, NPSP mainstream samples) were collected at the estuary of the NPSP tributary of the Jialing River. High-throughput sequencing of the fungal-specific internal transcribed spacer (ITS) gene region was used to identify fungal taxa. The impact of NPSP on the aquatic environment of the Jialing River was revealed by analysing the community structure, community diversity and functions of sediment fungi. The results showed that the dominant phylum of sediment fungi was Rozellomycota, followed by Ascomycota, Chytridiomycota, Basidiomycota, Mortierellomycota and Zoopagomycota (relative abundance>1%). NPSP caused a significant increase in the relative abundances of Rozellomycota, Saccharomycetes, Microascales, Saccharomycetales, Branch02 and Branch03. In addition, it caused a significant decrease in the relative abundances of Chytridiomycota, Dothideomycetes, Capnodiales, Glomerellales, Xylariales and Chaetothyriales. Moreover, NPSP caused significant changes in the physicochemical properties of Jialing River sediments, such as pH and available nitrogen (AN), which significantly increased the species richness of fungi and caused significant changes in the fungal community β-diversity (P<0.05). pH, total phosphorus (TP) and AN were the main environmental factors affecting fungal communities in Jialing River sediments. The functions of sediment fungi mainly involved three types of nutrient metabolism (symbiotrophic, pathotrophic and saprotrophic) and 75 metabolic circulation pathways. NPSP significantly improved the NONOXIPENT-PWY, PENTOSE-P-PWY, and PWY-6837 metabolic circulation pathway functions (P<0.05) and inhibited the PWY-7118, PWY-5920, and PWY-6609 metabolic circulation pathway functions (P<0.05). Hence, NPSP causes changes in the community structure and functions of sediment fungi in Jialing River and destroys the stability of the Jialing River Basin ecosystem.
Effective Use of Remote Learning in Sustaining Teaching and Learning of Fine and Applied Arts in COVID-19 Era in Colleges of Education
Authors: Ibenegbu Christopher Ifeanyi , Angu Juliet Bih 
Published: December 2021

Abstract
The study determined how remote learning we can use effectively in sustaining teaching fine and applied arts in colleges of education in Benue State, Kogi State, and Enugu State, Nigeria. We adopted a cross-sectional survey for the study. We used a sample size of 218 respondents for the study. We used a structured questionnaire for the study. We used frequency percentage and Chi-square analysis. We planned and tested four hypotheses. The finding of the study showed that the ICT devices are not available; respondents do not use the effective technique for teaching fine and applied arts in remote learning. The respondents found out that there are challenges concerning the use of technology in the teaching fine and applied arts, and respondents agree on the solutions in mitigating the adverse effect of COVID-19 lockdown on teaching fine and applied arts. We highlighted the educational implications of the findings and the recommendations. We also made some limitations of the study and suggestions for further studies.
Protein Structure Dynamic Prediction: a Machine Learning/Molecular Dynamic Approach to Investigate the Protein Conformational Sampling
Authors: Audagnotto Martina , Czechtizky Werngard , De Maria Leonardo , Käck Helena , Papoian Garegin , Tornberg Lars , Tyrchan Christian , Ulander Johan 
Published: December 2021

Abstract
Proteins exist in several different conformations. These structural changes are often associated with fluctuations at the residue level. Recent findings show that co-evolutionary analysis coupled with machine-learning techniques improves the precision by providing quantitative distance predictions between pairs of residues. The predicted statistical distance distribution from Multi Sequence Analysis (MSA) reveals the presence of different local maxima suggesting the flexibility of key residue pairs. Here we investigate the ability of the residue-residue distance prediction to provide insights into the protein conformational ensemble. We combine deep learning approaches with mechanistic modeling to a set of proteins that experimentally showed conformational changes. The predicted protein models were filtered based on energy scores, RMSD clustering, and the centroids selected as the lowest energy structure per cluster. The models were compared to the experimental-Molecular Dynamics (MD) relaxed structure by analyzing the backbone residue torsional distribution and the sidechain orientations. Our pipeline not only allows us to retrieve the global experimental folding but also the experimental structural dynamics. We show the potential correlation between the experimental structure dynamics and the predicted model ensemble demonstrating the susceptibility of the current state-of-the-art methods in protein folding and dynamics prediction and pointing out the areas of improvement.
Docked ions: vertical-aligned tubular arrays for highly efficient capacitive deionization
Authors: Luo Zhi-Yong , Wang Dong , Huang Lingqi , Liu Xiangyang , Zhang Qi , Zhu He , Zhu Shiping 
Published: December 2021

Abstract
Capacitive deionization (CDI) is an effective method for desalination of brackish water to alleviate the global freshwater crisis. Obtaining high desalination capacity is the primary focus of this field. Based on electrical double layer (EDL) theory, current research is mainly devoted to increasing the specific surface area of electrode materials, however, the NaCl adsorption capacity is typically limited to the range of 10 - 20 mg g−1. In this work, we propose a new design paradigm of using a vertical-aligned nanotubular structure for CDI. This design allows ions to be temporarily held inside the electrodes like ships docked in a harbor (ion-docking effect, IDE) due to the greatly diminished water flow inside the tubes, thus enhancing the desalination capacity. As a result, the obtained CDI device based on vertical-aligned nanotubular P-TiO2 arrays shows an ultra-high NaCl adsorption capacity of ~60 mg g−1 within 30 minutes in 0.01 mol L−1 NaCl solution under 1.2 V, corresponding to a rapid average adsorption rate of 2 mg g−1 min−1. Moreover, the adsorption capacity could be further increased up to 121 and 136 mg g−1 under 1.2 and 1.5 V for 2.5 hours adsorption, respectively, but still far from its equilibrium value. Finally, experiments and theoretical simulations are combined to further understand the IDE in CDI. This work highlights the discovery and the utilization of IDE in CDI, and provides new guidance for the design of CDI electrodes and can facilitate the development of CDI technology.
The Impact of Climate Change On Financing Cost of Heavy-Pollution Firms in China
Authors: Chang Kai , Nie Yixia 
Published: December 2021

Abstract
We examines the effects of climate change on the financing cost of heavy-pollution firms using firm-level panel data analysis. The empirical results demonstrate that the annual temperature and precipitation changes can significantly promote the financing costs of heavy-pollution firms, the positive impacts of annual temperature and precipitation changes on the financing costs of large, medium and small heavy-pollution firms has shown a gradual weakening trend with an increase of firm size, and the positive effects of annual temperature and precipitation changes on the financing costs of younger and older heavy-pollution firms has shown a decline trend with an increase of firm age. The evidences confirms that the impact of climate change on the financing costs of heavy-pollution firms exhibit a significant firm size and age discrimination of financing behaviors. Government decision-makers have to identify and optimize the transmission effect of climate change response on financing behavior decisions of heavy-pollution industries, financial institutions alleviate financial conflicts and credit discrimination behaviors and optimize the efficiency of financial resource allocation. Firms’ executives correct climate change strategy, optimize the climate- relevant operation, investment and financing activities, and alleviate unfavorable influences of climate changes for heavy-pollution firms.
Synthesis and preliminary biological evaluation of new phthalazinone derivatives with PARP-1 and cholinesterase inhibitory activities
Authors: Yu Lin , Chengzhi Gao , Zhuyong Wang , Ruifeng Zhang , Yajun Chen , Zhenli Min 
Published: December 2021

Abstract
The inhibition of Poly (ADP-ribose) polymerases-1 (PARP-1) has a potentially therapeutical value for AD. In order to search for a new agent based on multitarget-directed ligands (MTDLs) strategy, a series of 21 novel compounds incorporated the respective pharmacophores of two marketed drugs, namely 4-benzyl phthalazinone moiety of PARP-1 inhibitor Olaparib and N-benzylpiperidine moiety of AChE inhibitor Donepezil, into one molecule was synthesized. The inhibitory activities of all the synthesized compounds against the enzymes PARP-1, AChE and BChE were evaluated. Among them, 30 exhibited the most potent inhibitory effect on PARP-1 enzyme (8.18±2.81 nM) and moderate BChE inhibitory activity (1.63±0.52µM), while its AChE inhibitory activity (13.48±2.15µM) was weaker than Donepezil (0.04±0.01µM). Further molecular docking studies revealed that four hydrogen bonds were formed between 30 and PARP-1 which were similar with the interactions between Olaparib and PARP-1. 30 interacted with the critical residues His438 and Trp82 of huBChE through hydrogen bond and hydrophobic interaction which were necessary for huBChE inhibitory potency. Our research gave a clue to search for new agents based on PARP-1 and cholinesterase dual-inhibited activities to treat Alzheimer's disease.
An Ethical Issue After the Nuclear Accident in Fukushima: Young People’S Perspectives of Thyroid Cancer Screening and its Harms
Authors: Midorikawa Sanae , Ohtsuru Akira 
Published: December 2021

Abstract
Background

Overdiagnosis of thyroid cancer has become a major global medical issue. Ultrasound-based thyroid cancer screening has promoted overdiagnosis, and recently international recommendations indicate that such screening should not be conducted, even after a nuclear accident. The Fukushima thyroid cancer screening program was initiated in 2011 as a health policy after the nuclear accident, although the risk for radiation-induced thyroid cancer was unlikely given the low radiation levels. However, the thyroid cancer screening program has continued at 2-year intervals with a relatively high participation rate and is now in its fifth round. Therefore, it is crucial to clarify whether those targeted for screening understand the disadvantages of screening and identify factors that influenced their decision to participate.
Methods

We conducted an anonymous mail-based questionnaire that included young people from Fukushima (subjects) and a neighboring prefecture that was not targeted for screening (non-subjects). We asked them about the significance of the thyroid cancer screening in Fukushima, the reasons for taking or not taking a screening, their perception of the harms of screening, and their opinions on thyroid examination at school. Then we compared the results of the questionnaire between subjects and non-subjects and examinees (who accepted screening) and non-examinees (who declined screening).
Results

Only 16.5% of respondents were aware of the harms associated with thyroid cancer screening, with most perceiving that the benefits outweighed the harms. Comparison of subjects’ and non-subjects’ responses showed there were no significant differences between subjects and non-subjects. In addition, among subjects, there were no differences of responses between examinees and non-examinees. The most common reason for participation in screening was that the screening was conducted in schools and perceived as obligatory.
Conclusions

These results highlighted a serious ethical issue in that school-based screening leads to making young people think that it is mandatory screening in an opt-out and default setting manner, with a lack of knowledge about the disadvantages of screening. Based on the autonomy of the subjects and the ethical principle of the post-disaster, surveys after a nuclear disaster should be conducted in an opt-in style without an opt-out style such as school-based screening.