bluuebunny/crossref_metadata_2025_split huggingface dataset:
A random small molecule library screen identifies novel antagonists of the kinin receptor from the cattle fever tick, Rhipicephalus microplus (Acari: Ixodidae)
Authors: Xiong Caixing , Baker Dwight , Pietrantonio Patricia V 
Published: January 2021

AbstractBACKGROUNDThe southern cattle tick, Rhipicephalus microplus, is a primary vector of the deadly bovine disease babesiosis. Worldwide populations of ticks have developed resistance to acaricides, underscoring the need for novel target discovery for tick control. The arthropod‐specific R. microplus kinin receptor is such a target, previously validated by silencing, which resulted in female reproductive fitness costs, including a reduced percentage of eggs hatching.RESULTSIn order to identify potent small molecules that bind and activate or inhibit the kinin receptor, a high‐throughput screening (HTS) assay was developed using a CHO‐K1 cell line expressing the recombinant tick kinin receptor (BMLK3). A total of ~20 000 molecules from a random in‐house small molecule library were screened in a ‘dual‐addition’ calcium fluorescence assay. This was followed by dose–response validation of the hit molecules identified both from HTS and an in silico screen of ~390 000 molecules. We validated 29 antagonists, 11 of them were full antagonists with IC50 values between 0.67 and 8 μmol L–1. To explore the structure–activity relationships (SAR) of the small molecules, we tested the activities of seven analogs of the most potent identified antagonist, additionally discovering three full antagonists and four partial antagonists. These three potent antagonists (IC50 < 3.2 μmol L–1) were validated in vitro using the recombinant mosquito kinin receptor and showed similar antagonistic activities. In vivo, these three compounds also inhibited the mosquito hindgut contraction rate induced by a myotropic kinin agonist analog 1728.CONCLUSIONAntagonists identified in this study could become pesticide leads and are reagents for probing the kinin signaling system. © 2021 Society of Chemical Industry
Integrating out new fermions at one loop
Authors: Angelescu Andrei , Huang Peisi 
Published: January 2021

Abstract
We present the fermionic universal one-loop effective action obtained by integrating out heavy vector-like fermions at one loop using functional techniques. Even though previous approaches are able to handle integrating out heavy fermions with non-chiral interactions, i.e. vanishing γ5 interaction terms, the computations proceed in a tedious manner that obscures a physical interpretation. We show how directly tackling the fermionic functional determinant not only allows for a much simpler and transparent computation, but is also able to account for chiral interaction terms in a simple, algorithmic way. Finally, we apply the obtained results to integrate out at one loop the vector-like fermions appearing in a toy model and in a fermionic model that exhibits strong cosmological phase transitions.
A Two-Parameter Corresponding States Method for Calculating the Steady-State Evaporation Rate of C2–C9 n-Alkane Droplets in Air for Elevated Pressures and Temperatures
Authors: Csemány Dávid , Józsa Viktor 
Published: January 2021

AbstractAdvanced gas turbine and internal combustion engine combustion chambers operate at highly elevated pressures and temperatures. Therefore, spray vaporization analysis cannot be limited to the atmospheric environment since evaporation strongly depends on ambient conditions. Presently, the effect of air pressure and temperature on droplet evaporation rate was investigated by using both a transient and a steady-state approach. A corresponding states model was derived for the steady-state evaporation rate for n-alkanes in the range of C2–C9 with an excellent fit quality and < 1% model uncertainty, considering the thermophysical data uncertainties. The model was tested for C1, C10, and C12 n-alkanes as well with low success. The ambient conditions were evaluated in terms of reduced pressures and temperatures, covering the range of 0.02–0.5 and 1.2–1.5, respectively. However, the applicability of the model was limited to reduced temperature of 1.3–1.5, as higher discrepancy was observed between the trends of the different n-alkanes at lower temperatures. Since the heat-up phase of practical sprays in combustion chambers is often short, the present model might significantly reduce the computational effort required for liquid evaporation calculations.
Long Non-coding RNA FEZF1-AS1 Promotes Growth and Reduces Apoptosis Through Regulation of miR-363-3p/PAX6 Axis in Retinoblastoma
Authors: Liu Xiuming , Li Xiaofeng , Li Jianchang 
Published: January 2021

AbstractRetinoblastoma is the most common malignancy in children's eyes with high incidence. Long non-coding RNAs (lncRNAs) play important roles in the progression of retinoblastoma. LncRNA FEZF1 antisense RNA 1 (FEZF1-AS1) has been found to stimulate retinoblastoma. However, the mechanism of FEZF1-AS1 underlying progression of retinoblastoma is still unclear. In current study, FEZF1-AS1 was up-regulated in retinoblastoma tissues and cells. FEZF1-AS1 overexpression enhanced retinoblastoma cell viability, promoted cell cycle, and inhibited apoptosis. Conversely, FEZF1-AS1 knockdown reduced cell viability, cycle, and elevated apoptosis. The interaction between FEZF1-AS1 and microRNA-363-3p (miR-363-3p) was confirmed. FEZF1-AS1 down-regulated miR-363-3p and up-regulated PAX6. PAX6 was a target gene of miR-363-3p. EZF1-AS1 promoted retinoblastoma cell viability and suppressed apoptosis via PAX6. Further, we demonstrated that FEZF1-AS1 contribute to tumor formation in vivo. In conclusion, FEZF1-AS1 elevated growth and inhibited apoptosis by regulating miR-363-3p/PAX6 in retinoblastoma, which provide a new target for retinoblastoma treatment.
Pecunia non olet: on the self-selection into (dis)honest earning opportunities
Authors: Konrad Kai A. , Lohse Tim , Simon Sven A. 
Published: January 2021

AbstractWe study self-selection into earning money in an honest or dishonest fashion based on individuals’ attitudes toward truthful reporting. We propose a decision-theoretic framework where individuals’ willingness to pay for honest earnings is determined by their (behavioral) lying costs. Our laboratory experiment identifies lying costs as the decisive factor causing self-selection into honest earning opportunities for individuals with high costs and into cheating opportunities for those prepared to misreport. Our experimental setup allows us to recover individual lying costs and their distribution in the population.
High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic
Authors: Porcu Eleonora , Tranquillo Maria Lucrezia , Notarangelo Leonardo , Ciotti Patrizia Maria , Calza Nilla , Zuffa Silvia , Mori Lisa , Nardi Elena , Dirodi Maria , Cipriani Linda , Labriola Francesca Sonia , Damiano Giuseppe 
Published: January 2021

AbstractPurposeThe main purpose and research question of the study are to compare the efficacy of high-security closed versus open devices for human oocytes’ vitrification.MethodsA prospective randomized study was conducted. A total of 737 patients attending the Infertility and IVF Unit at S.Orsola University Hospital (Italy) between October 2015 and April 2020 were randomly assigned to two groups. A total of 368 patients were assigned to group 1 (High-Security Vitrification™ - HSV) and 369 to group 2 (Cryotop® open system). Oocyte survival, fertilization, cleavage, pregnancy, implantation, and miscarriage rate were compared between the two groups.ResultsNo statistically significant differences were observed on survival rate (70.3% vs. 73.3%), fertilization rate (70.8% vs. 74.9%), cleavage rate (90.6% vs. 90.3%), pregnancy/transfer ratio (32.0% vs. 31.8%), implantation rate (19.7% vs. 19.9%), nor miscarriage rates (22.1% vs. 21.5%) between the two groups. Women’s mean age in group 1 (36.18 ± 3.92) and group 2 (35.88 ± 3.88) was not significantly different (P= .297). A total of 4029 oocytes were vitrified (1980 and 2049 in groups 1 and 2 respectively). A total of 2564 were warmed (1469 and 1095 in groups 1 and 2 respectively). A total of 1386 morphologically eligible oocytes were inseminated by intracytoplasmic sperm injection (792 and 594 respectively,P= .304).ConclusionsThe present study shows that the replacement of the open vitrification system by a closed one has no impact on in vitro and in vivo survival, development, pregnancy and implantation rate. Furthermore, to ensure safety, especially during the current COVID-19 pandemic, the use of the closed device eliminates the potential samples’ contamination during vitrification and storage.
Biology Undergraduate Students’ Graphing Practice in Digital Versus Pen and Paper Graphing Environments
Authors: Gardner Stephanie M. , Suazo-Flores Elizabeth , Maruca Susan , Abraham Joel K. , Karippadath Anupriya , Meir Eli 
Published: January 2021

AbstractGraphing is an important practice for scientists and in K-16 science curricula. Graphs can be constructed using an array of software packages as well as by hand, with pen-and-paper. However, we have an incomplete understanding of how students’ graphing practice vary by graphing environment; differences could affect how best to teach and assess graphing. Here we explore the role of two graphing environments in students’ graphing practice. We studied 43 undergraduate biology students’ graphing practice using either pen-and-paper (PP) (n = 21 students) or a digital graphing tool GraphSmarts (GS) (n = 22 students). Participants’ graphs and verbal justifications were analyzed to identify features such as the variables plotted, number of graphs created, raw data versus summarized data plotted, and graph types (e.g., scatter plot, line graph, or bar graph) as well as participants’ reasoning for their graphing choices. Several aspects of participant graphs were similar regardless of graphing environment, including plotting raw vs. summarized data, graph type, and overall graph quality, while GS participants were more likely to plot the most relevant variables. In GS, participants could easily make more graphs than in PP and this may have helped some participants show latent features of their graphing practice. Those students using PP tended to focus more on ease of constructing the graph than GS. This study illuminates how the different characteristics of the graphing environment have implications for instruction and interpretation of assessments of student graphing practices.
The impact of closing schools on working from home during the COVID-19 pandemic: evidence using panel data from Japan
Authors: Yamamura Eiji , Tsustsui Yoshiro 
Published: January 2021

AbstractCOVID-19 has led to the closure of various schools in Japan to cope with the pandemic. This study explores how school closure influences parents’ work style based on short panel data for the period of school closure from mid-March to mid-April 2020. Specifically, we analyze how the presence of their children influences parents’ work at home and examine how the effect differs by the parent’s gender. After controlling for various factors, we find that in cases where parents are full-time employees and the children are: (1) in primary school, mothers are more likely to work remotely, while fathers are less likely to do so and (2) in junior high school, the parents’ work styles are hardly affected. This shows that mothers shoulder the burden of working remotely and caring for small children at home, while fathers tend to work in the office and spend less time with their childcare at home. Inevitably, COVID-19 has increased the inequality in the burden of child care.
Interactive Effects of Copper Sources and a High Level of Phytase in Phosphorus-Deficient Diets on Growth Performance, Nutrient Digestibility, Tissue Mineral Concentrations, and Plasma Parameters in Nursery Pigs
Authors: Ren Ping , Chen Juxing , Hancock Deana , Vazquez-Añón Mercedes 
Published: January 2021

AbstractThe present study investigated the interactive effects of copper sources and a high level of phytase on growth performance, nutrient digestibility, tissue mineral concentrations, and plasma parameters in nursery pigs. Weaning piglets (N = 192; 6.06 ± 0.99 kg), blocked by body weight, were randomly allotted to 1 of 4 dietary treatments, with 12 pens per treatment and 4 pigs per pen. A basal diet for each phase was formulated to meet nutrient requirements for nursery pigs with the exception that standardized total tract digestibility (STTD) P was reduced by 0.12% and Ca was adjusted to achieve Ca/STTD P = 2.15. The 4 dietary treatments were arranged in a 2 × 2 factorial design, with 2 Cu sources (125 mg/kg Cu from copper methionine hydroxy analogue chelate (Cu-MHAC) or copper sulfate (CuSO4)) and 2 phytase levels (0 or 1500 phytase units (FTU)/kg). Results showed that there was an interaction (P < 0.05) between Cu sources and phytase on ADG during days 0–41. When phytase was not present in the diets (P deficient), there was no difference between the two Cu sources in terms of ADG during days 0–41, whereas with phytase in the diets, Cu-MHAC tended to improve (P < 0.10) ADG during days 0–41 compared with CuSO4. Pigs fed Cu-MHAC had greater apparent total tract digestibility (ATTD) of neutral and acid detergent fiber and STTD of P than those fed CuSO4. Phytase increased (P < 0.05) growth performance, ATTD of Ca and P, and plasma inositol and growth hormone concentrations. In conclusion, Cu-MHAC may be more effective in improving growth rate than CuSO4 when phytase was supplemented at 1500 FTU/kg. Cu-MHAC enhanced fiber and P digestibility regardless of phytase, compared with CuSO4. Phytase addition in P-deficient diets was effective in improving growth performance, Ca and P digestibility, and plasma inositol and growth hormone concentrations.
Critical role of lipid membranes in polarization and migration of cells: a biophysical view
Authors: Sackmann Erich , Tanaka Motomu 
Published: January 2021

AbstractCell migration plays vital roles in many biologically relevant processes such as tissue morphogenesis and cancer metastasis, and it has fascinated biophysicists over the past several decades. However, despite an increasing number of studies highlighting the orchestration of proteins involved in different signaling pathways, the functional roles of lipid membranes have been essentially overlooked. Lipid membranes are generally considered to be a functionless two-dimensional matrix of proteins, although many proteins regulating cell migration gain functions only after they are recruited to the membrane surface and self-organize their functional domains. In this review, we summarize how the logistical recruitment and release of proteins to and from lipid membranes coordinates complex spatiotemporal molecular processes. As predicted from the classical framework of the Smoluchowski equation of diffusion, lipid/protein membranes serve as a 2D reaction hub that contributes to the effective and robust regulation of polarization and migration of cells involving several competing pathways.
Interferences of trace metals between sediment and Dotillid crab (Ilyoplax frater) from three tidal creeks, Karachi, Pakistan
Authors: Siddiqui Asmat Saleem , Saher Noor Us 
Published: January 2021

AbstractIn the Anthropocene era, an immersion of toxic substances, i.e., trace metals, has been enhanced in the marine environment not only due to urban sprawl and industrial development but predominantly owing to incongruous management and lack of sustainable approaches. The coastal region of Pakistan shares a similar obstacle as most of the developing countries confronted. Therefore, this study was designed to investigate concentrations of eight metals (Cu, Fe, Zn, Ni, Co, Pb, Cr, and Cd) in sediment and Dotillid crab,Ilyoplax frater, at three tidal creeks in Karachi, Pakistan. All metals in sediments and crabs were analyzed by atomic absorption spectrometer. The sediment pollution was evaluated by contamination degree (CD) and potential ecological risk index (RI). After depicting the metal pollution in sediments, metal accumulation, and contamination in benthic crab were investigated through total metal concentrations in crabs, accumulation factor (AF), correlation analysis, and regression analysis. The results exhibited substantial differences in the concentrations of Zn, Ni, Pb, Cr, and Cd among the tidal creek sediments. Contamination factors indicated that the Cd and Pb had the highest sharing in sediment pollution, and the tidal creeks classified as moderately contaminated. All metal accumulations in Dotillid crabs showed notable spatial variations, and accumulation factors (AFs) for most of the metals were > 1.0, signifying the strong bioaccumulation of metals in crabs. Particularly, Cu, Co, and Cd levels were considerably greater (two to three times) in Dotillid crabs compared to creek’s sediments, even though they don’t share any relationship between two matrixes. Hydrographical and sedimentological traits also revealed significant interactions with metal levels in sediments and crabs. A substantial association was also noticed in Fe, Ni, and Pb between sediments and crabs. Interestingly, most of the metal AFs showed a notable inverse correlation with the environmental matrix. Exceptionally, a strong positive correlation found between the Pb concentration in crabs and sediments suggested thatI. fraterprobably acts as an indicator of Pb pollution.
Parametric study of a corrugated airfoil in a forward flight at ultra-low Reynolds number
Authors: Abd El-Latief Mahmoud E. , Elsayed Khairy , Abdelrahman Mohamed M. 
Published: January 2021

AbstractIn the current study, the mid cross section of the dragonfly forewing was simulated at ultra-low Reynolds number. The study aims to understand better the contribution of corrugations found along the wing on the aerodynamic performance during a forward flight. Different flapping parameters were employed. FLUENT solver was used to solve unsteady, two-dimensional, laminar, incompressible Navier–Stokes equations. The results revealed that any stroke amplitude less than 1cmgenerated no thrust force. The stroke amplitude had to be increased to form the reversed Kármán vortices responsible for generating thrust force. The highest propulsive efficiency was found in the Strouhal number range 0.2 < St < 0.4 with a peak efficiency of 57% atSt = 0.39. Changing the phase difference between pitching and plunging motions from advanced to synchronized caused lift force to drop 91% and thrust force to increase by 15%. On the other hand, changing the phase difference from synchronized to delayed caused lift and thrust forces to increase by 89% and 36%, respectively, and propulsive efficiency to deteriorate significantly. In all performed simulations, the airfoil was assumed to start motion from rest with no initial angle of attack. The increase in initial angle of attack generates a very high lift force with a fair loss for both thrust force and propulsive efficiency. The decomposition of flapping motion into its elementary motions revealed that the aerodynamic forces generated are a non-linear superposition from both pure pitching and pure plunging aerodynamic forces. This can be attributed to the non-linear interaction between unsteady vortices generated from these decomposed motions.
Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1
Authors: Poddar Kasturi , Padhan Bhagyashree , Sarkar Debapriya , Sarkar Angana 
Published: January 2021

AbstractPigment-producing bacteria were isolated from kitchen wastewaters of the National Institute of Technology, Rourkela. A pink non-virulent bacterial strain PWN1 was selected based on the India Ink Broth and Coomassie Brilliant Blue (R-250) dye assay. According to morphological and biochemical characterization, the strain PWN1was a Gram-negative, rod-shaped, motile, non-coliform bacterium and could utilize only glucose and adonitol as sole carbon source. The pigment was found to be a growth-associated product, and the pigment production was accelerated after 40 h of bacterial culture. Further, 16S rRNA gene-based molecular identification showed its similarity with Enterobacter sp. The pigments were extracted by the solvent extraction method using chloroform and ethanol (3:1). The extracted pigments were then purified through thin-layer chromatography and column chromatography. To maximize pigment production, the culture condition was optimized for maximum biomass production using statistical software Design Expert v13. A quadratic model was structured describing the process efficiently and it suggested a moderate temperature, pH, and a high inoculum concentration which generated biomass of 3.81 ± 0.02 g/L. At optimized condition, 1 L of cell culture produced 3.77 g of biomass which produced a crude pigment of 0.234 g after solvent extraction and 0.131 g after column chromatography, implying a yield of 6.2% for crude pigment and 3.47% for purified pigment from biomass. The yield of the obtained pigment was high enough to draw interest for industrial production, although the application of the pigment is considerable for further study.
Is Clitoral Release Another Term for Metoidioplasty? A Systematic Review and Meta-Analysis of Metoidioplasty Surgical Technique and Outcomes
Authors: Jolly Divya , Wu Catherine A. , Boskey Elizabeth R. , Taghinia Amir H. , Diamond David A. , Ganor Oren 
Published: January 2021

Abstract

Introduction
There has been an exponential increase in referrals for transmasculine patients seeking genital affirmation surgery. Despite transgender men’s equal interest in metoidioplasty and phalloplasty, research has primarily focused on phalloplasty.

Aim
To summarize and investigate the relationship between surgical technique, complications, and patient-satisfaction.

Methods
We performed a systematic review and meta-analysis of surgical techniques and physician- and patient-reported outcomes of gender-affirming clitoral release and metoidioplasty (PROSPERO# 158722) with literature from PubMed, Google Scholar, and ScienceDirect. Data were extracted using PRISMA guidelines. All searches, extractions, and grading were independently completed by 2 authors.

Main Outcome Measures
Main measures were surgical technique, patient satisfaction, voiding, urethral stricture, and urethral fistula.

Results and Conclusion
A total of 7 non-overlapping articles on metoidioplasty were identified, with a total of 403 patients. We identified 4 metoidioplasty techniques: Hage, Belgrade, labial ring flap, and extensive metoidioplasty. All techniques included urethral lengthening. The reported neophallus length ranged from 2 cm to 12 cm, with the smallest neophallus occurring with the labial ring flap technique and extensive metoidioplasty the largest. Across techniques, voiding while standing was reported in most patients, with the lowest rate reported with the labial ring flap (67%). Complications were impacted by surgical technique, with the lowest rates of fistula and stricture occurring with the Belgrade technique. Fistula rates ranged from 5% to 37%, while stricture ranged from 2% to 35% of patients. The Belgrade technique reported significantly lower rates of fistula and stricture (P = .000). The patient-reported outcomes were described for the Belgrade technique and extensive metoidioplasty. Both techniques showed high aesthetic and sexual satisfaction. Transgender individuals can achieve an aesthetically and sexually satisfactory neophallus using a variety of metoidioplasty techniques; however, urethral outcomes vary significantly by technique. The Belgrade technique reported the best outcomes, although data remains limited. Patient priorities should be used to determine surgical technique.