bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution of Four Biofilm Associated Gene among A. baumannii by in Silico-PCR
Authors: Babu J. D. Monesh , Girija A. S. Smiline , Ganesh P. Sankar , Priyadharsini J. Vijayashree 
Published: December 2021

Background: A.baumannii is an opportunistic pathogen known for its efficient biofilm formation that is attributed for its virulence. Acinetobacter baumannii is an inhabitant of oral biofilms as well. Many gene operons are involved in the biofilm formation that need to be monitored frequently.
Aim: The aim of the present study was to detect the distribution of four biofilm associated genes among A.baumannii.
Materials and Methods: Four biofilm forming genes viz., bfms, ptk, pgaB, and fimH of A.baumannii were selected. Forward and reverse primers of those four genes were used for in-silico PCR amplification. 19 strains of A.baumannii set as default on the server were chosen and the amplicon bands were observed
Results: The present investigation documents the distribution of four vital biofilm associated gene among 19 different strains of A.baumannii among which bfms was distributed at a higher frequency followed by pgaB and ptk
Conclusion: The finding of the study suggests the presence of pgaB, bfms, ptk among the 19 different strains of A.baumannii. However further experimental validation must be done to frequently monitor the presence of the genes among the clinical strains of A.baumannii.
Case Report on Alcohol Dependence with Delirium Tremors
Authors: Kakade Rohini , Gujar Samrudhhi , Pohekar Savita , Sharma Ranjana , Ankar Ruchira , Tembhare Vaishali , Morye Swapna , Wavare Sonali 
Published: December 2021

Alcohol is a natural product made by reacting lactose fermentation with yeast spores. Because it includes calories. With no nutritional value, alcohol is used as a source of energy Different sugar sources are used for fermentation to create a variety of alcoholic beverages. Patient Present History:-On September 3rd, 2020, a 32-year-old male patient was admitted to hospital with a specific complaint of abdominal pain for the past three years. He began drinking alcohol on a daily basis. Initially, he only drank at night, but later he began drinking during the day.
Clinical Finding: Mood irritable, social withdrawal, mental confusion, seizure, vomiting, mood changes, lethargy and vomiting.
Diagnostic Evaluation: Hb - 11.9 mg%, MCV – 87 cum/micron, MCH- 28.9 pico/gm, Total Bilirubin- 1.47.USG –He is appearance was natural once more. There was no localized lesion or oblivious mass lesion in the pelvis.
Conservative Rehabilitation of Partial Anterior Cruciate Ligament Tear - A Case Report
Authors: Uttamchandani Shivani , Bhageriya Khushboo , Deshmukh Mithushi 
Published: December 2021

The anterior cruciate ligament is one of the most important ligaments in the knee joint (ACL). The ACL is necessary for appropriate knee joint mobility and mechanics. When the ACL is torn, patients may experience issues with their knee's function, as well as instability and the sensation of 'falling away' while walking. The anterior cruciate ligament acts as a main restraint on tibia-to-femur translation (anterior shear). Depending on the angle of knee flexion, this function is allocated to either anteromedial or posterolateral bundle. When the knee is fully extended, the posterolateral bundle is tense; however, when the knee flexes, the posterolateral bundle loosens and the anteromedial bundle tightens.
We report the case of a 32-year-old man who twisted his leg while jogging, resulting in a partial thickness ACL rupture and mild joint effusion along the periarticular surface of the right knee, as revealed by radiological symptoms and MRI.Conservative rehabilitation in a patient aged 32 year shows good results and so the surgery was not performed. Initially 10 weeks protocol was managed in hospital under the guidance of a therapist including strengthening, stretching and conditioning of lower limb muscles, later on as pain reduced and patient achieved functional pain free range 12 week protocol was further set for home program.
A Case Report on Alcohol Dependence Syndrome with Cannabis Addiction
Authors: Ekhar Deepti , Vanlalpeka Samual , Sayyad Shabnam , Meshram Dharti , Uke Trupti , Gawai Jaya , Kasturkar Pooja 
Published: December 2021

Background: Alcohol dependence syndrome (ADS) and Cannabis use disorder (CUD) are every now and again co-happening or comorbid substance issues in the two young people and grown-ups. Indications of cross-over between ADS and CUD in people as they slowly move from pre-adulthood to adulthood. The researchers reasoned that the two substance-related issues are comorbid, albeit the closeness of their association ordinarily shifts over the long haul.
Aim and Objective: The purpose of this case report is to determine the first-line approach for a person with alcohol dependence syndrome with cannabis addiction who has been referred to a public mental health facility for treatment. To identifying symptoms of ADS with cannabis addiction early, providing treatment and preventing possible complications.
Methods: Knowledge used to write this case description was gathered from PubMed outlets, search hand, searching college and personal libraries looking for research techniques and case report texts, engaging in or writing many case reports with experience.
Results: The patient was taken psychopharmacological treatment Anti- Anxiety drugs Lorazepam along with Tab. Benalgis, Tab. Neurobion fort and psycho social therapy, coping strategies, family therapy, yoga, cognitive behavioural therapy, medication. After those symptoms was minimized.
Conclusion: Patients achieve positive outcomes not only through the support of their treatment management, but also through adaptation and family support. Then, with appropriate psychophysiological treatment, the patient gave a positive response and gradually all the planned goals were achieved. Finally, the patient was discharged and he is currently being monitored.
Causes and Management of Acute Pyelonephritis
Authors: Hussein Mohammed Salah , Almukalaf Jawaher Abdullah , Alalyani Saad Mohammed , Alharbi Rayan Mofareh , Alzahrani Wejdan Ibrahim , Aldhubiani Duaa Salah , Khubrani Adel Ali , Alharbi Ziad Mofareh , Alotaibi Leena Saqer , Alradhi Zainab Mohamed , Alharbi Ghoson ghonem , Alrumyyan Rumyyan Abdulaziz Rumyyan 
Published: December 2021

Acute pyelonephritis is a bacterial infection that causes kidney inflammation. Pyelonephritis is a kidney infection that develops as a result of an ascending urinary tract infection that travels from the bladder to the kidneys. Acute pyelonephritis affects over 250,000 people each year, resulting in more than 100,000 hospitalizations. Infection with Escherichia coli is the most prevalent cause. Fever, vomiting, abdomen or loin discomfort, and fatigue are all symptoms of acute pyelonephritis, however Fever is the most clinically useful symptom. Escherichia coli is the causative agent in more than 80% of instances of acute pyelonephritis. Staphylococcus saprophyticus, and enterococci are among the other etiologic factors. While Infections caused by Klebsiella, Enterobacter, Clostridium, or Candida are more common in diabetic patients. Acute pyelonephritis can be treated as an outpatient or as an inpatient procedure. Outpatient treatment is available for healthy, young, non-pregnant women with uncomplicated pyelonephritis. The choice of first-line oral antibiotics depends on local antibiotic resistance characteristics, although trimethoprim alone or in combination with sulphamethoxazole, cephalexin, or amoxicillin-clavulanic acid. In this article we will be looking the causes and management of acute pyelonephritis.
A Case Report on Schizophrenia with Autoimmune Encephalitis
Authors: Meshram Dharti , Wavare Sonali , Uke Trupti , Sayyad Shabnam 
Published: December 2021

Schizophrenia is a severe mental illness with a high death rate and significant societal implications. Curative treatments are not available due to a lack of understanding of its etiopathogenesis. The mild encephalitis hypothesis of schizophrenia, established primarily by Karl Bechter and Norbert Müller, is one of the new research hypotheses. According to this theory, a significant subset of schizophrenia patients suffers from a mild but persistent form of encephalitis caused by a variety of etiology ranging from viral infections to traumas to autoimmune illnesses. This inflammatory method is believed to occur in the start or during the course of the disease.
The authors present case of a 65-year-old female got admitted in female psychiatric ward AVBR Hospital Sawangi Meghe, Wardha Maharashtra with chief complaint of forgetfulness, interest in environment decline, unable to communicate, poor performance at work, muttering to self, sleep disturbance, seeing people which are not seen other, fearfulness. all necessary investigation done, in mental status examination founded impairment in memory, disorientation cognitive function impairment, RBC count 3.82, WBC count 5300, Hb% 12, calcium 8.1, urea 26, creatinine 0.6, sodium 142, potassium 4.0. Alkaline phosphate 89. HIV, HBSAG non-reactive, A large number of white blood cells in the CSF An MRI that reveals evidence of brain inflammation. There was a slight increase in antinuclear antibody (1: 40 titer). Blood and CSF were positive for oligoclonal bands. The patient was received symptomatic treatment antianxiety, antipsychotic drug alleviates hallucinations and delusion.
Disturbances of consciousness and orientation, catatonia, speech dysfunction, focal neurological signs, epileptic seizures/EEG abnormalities or autonomic dysfunction are warning signs in psychiatric patients which should always induce cerebrospinal fluid analysis with determination of antineuronal autoantibodies. Currently established immunotherapy strategies are summarised, taking into account international expert advice. Guided by clinical warning signs, our qualitative review enables rapid and reliable diagnosis of definite autoimmune encephalitis. This is of high relevance for the affected individuals, since early and sufficiently intense immunotherapy often leads to a good prognosis despite severe illness.
Effect of Box Breathing Technique on Lung Function Test
Authors: Ahmed Ashfaaq , Devi R. Gayatri , Priya A. Jothi 
Published: December 2021

Background: Box breathing exercise is a technique of slow breathing rhythm also known as square breathing used universally. It increases the performance and concentration level, it also acts as a powerful stress relief. It is tested with a spirometer for the lung function test that measures the airflow of the upper and lower respiratory tract. This method builds up carbon dioxide in blood which calms down and regulates the ANS (Autonomic Nervous System), which enhances the cardio- inhibitory response of the vagus nerve that improves the mood. The main aim of the study was to analyze the effect of box breathing technique on lung function
Materials and Methods: The present study was carried out among the participants at the age of 18-25 years from Saveetha Dental College. A total of 30 participants were included in this study (15 males, 15 females). The experimental training procedures consisted of 2 sessions of box breathing day and night for a period of 30 days. Participants were informed of the procedures of the box breathing technique. RMS Helios spirometer was used to measure lung volumes and capacities. Statistics analysis was done using SPSS software, paired t test. P value of less than or equal to 0.05 was considered significant statistically.
Results: There was a significant increase among the participants FVC (Forced Vital Capacity), FEV1 (Forced Expiratory Volume), FIVC (Forced Inspiratory Vital Capacity) parameters after 2 sessions on each day of box breathing technique for a period of 30 days. Other parameters increased to 10% but not significantly.
Conclusion: The present study illustrates the potential for box breathing practice to improve lung performance and reduce stress in healthy individuals. Despite the significant difference in FVC, FEV, FIVC levels supporting improved lung function, further investigation is needed to delineate mechanisms that underlie these benefits
Effect of Ocimum Sanctum Bio Compounds against csuE Gene Protein of Acinetobacter baumanii
Authors: Sunil Mukil , Girija A. S. Smiline , Ganesh P. Sankar , Priyadharshini J. Vijayashree 
Published: December 2021

Background: Acinetobacter baumannii is a Gram-negative bacillus that is aerobic, pleomorphic and non-motile. Multi-drug resistance and biofilm formation contributes to the virulence and pathogenicity of the bacterium. Among many virulence factors, csuE is critical for initiation and assembly, showing much homology to type 1 and P pili. With much propensity of drug resistance, in recent years alternative medications have spurred renewed interest in targeting potent pathogens. Ocimum sanctum, also known as holy basil or tulsi possess various bio-active properties and can be used as alternative medicine to treat systemic ailments.
Aim: This study was aimed to analyze the drug-ligand interactions between csuE protein of A. baumannii and the bio-compounds from O.sanctum using in-silico docking analysis.
Material and Methods: csuE protein was retrieved and optimisation of protein was done. Ligands were selected and were assessed for drug likeness using molinspiration parameters. Further the compounds were subjected for docking analysis and the interacted molecules were visualized for binding energy and hydrogen bonds.
Results: Out of the 9 compounds of Ocimum sanctum, benzofuran showed good interaction with csuE protein of Acinetobacter baumannii with a least docking energy of -5.31Kcal/Mol.
Conclusion: The present study recommends benzofuran as the potent candidate for novel drug design to treat the infections caused by A.baumannii upon further evaluations for its safety and immunological response.
Distribution of Biofilm-associated Genes among Acinetobacter baumannii by in-silico PCR
Authors: Joshua A. Aldrin , Girija A. S. Smiline , Ganesh P. Sankar , Priyadharsini J. Vijayashree 
Published: December 2021

Background: Acinetobacter baumannii is a coccobacillus that is Gram negative, non motile, non fermentative and oxidase negative. It is the most common and successful nosocomial pathogen recognised by WHO. This dreadful pathogen causes urinary tract infections, ventilator associated pneumonia (VAP), bacteremia, etc., These infections are most common in hospital wards especially Intensive Care Unit (ICU). The infections are due to biofilm formation by the virulent genes of A. baumannii, and the common biofilm-associated genes of A. baumannii were bap, csuE, fimH, epsA, bfmS, ptk, pgaB, ompA, blaPER-1. Among these, bap, epsA and ompA genes are highly prevalent among the clinical strains of A. baumannii.
Aim: To detect the three vital biofilm-associated genes of A. baumannii by in-silico PCR analysis.
Materials and Methods: 19 isolates of A. baumannii were selected and 3 target genes, namely epsA, ompA and bap gene were used for the amplification process through in-silico PCR simulation tools. Evolutionary analysis was done for the ompA gene.
Results: The epsA gene was expressed in 10.52% of the total strains selected with the highest occurrence of ompA gene as 57.89%. bap gene was not observed from the study strains included. From evolutionary analysis based on ompA distributed strains, the Acinetobacter baumannii SDF and Acinetobacter baumannii BJAB0715 might be the parental strains where the evolution of strains would have started. Through successive generations, the Acinetobacter baumannii MDR-ZJ06 and Acinetobacter baumannii TYTH-1 had become the multidrug resistant strains present in the environment.
Conclusion: The findings of the study confirms the distribution of epsA and ompA genes among the 19 different strains of A. baumannii. The study suggests periodical monitoring of biofilm based virulence genes among the clinical strains and to curtail the A. baumannii infections.
Guillain Barre Syndrome: A Case Report and Literature Review
Authors: Joshi Ragini , Mendhe Deeplata , Wanjari Mayur 
Published: December 2021

Introduction: The arrival of Guillain-Barre Syndrome is sudden. It’s a type of neuropathy caused by the immune system. Nutrition is very crucial. In impoverished countries, it is a disabling disease. Autoantibodies against diverse antigens can be seen in the outlying site. The occurrence links 0.4 to 1.7 million individuals per year.
Case Presentation: A 5-year-old boy was taken to the hospital with chief complaints of Weakness in bilateral upper and lower limbs, trouble in swallowing, inability to hold the neck, mouth-frothing, fever spikes. On physical examination, the patient has experienced weakness in bilateral upper and lower limbs, Bulbar weakness is present, pain experienced in both legs, the gag reflex is absent, In Cardiovascular System, S1 and S2 sound are present, In Respiratory System, Air entry is bilaterally equal, pupils are reflected light, tone, and power of upper and lower limbs are decreased, then treatment was started as soon as possible, he has not improved after receiving treatment, and the patient is on ventilator support, with treatment continuing until the end of my care.
Conclusion: In this study, we primarily focus on professional management, and outstanding nursing care may give the holistic care that Guillain Barre Syndrome requires while also effectively managing the challenging case. The comprehensive health care team collaborates to help the patient achieve their prior level of independence and satisfaction after a full recovery.
Digital Teaching Methodology among the Dental Students a Questionnaire Based Survey
Authors: Dharaneesh N. , Priya A. Jothi , Devi R. Gayatri 
Published: December 2021

Background: The implementation of digital technologies in dental curricula has started globally and reached varying levels of penetration counting on local resources and demands. One of the biggest challenges in digital education is the need to continuously adapt and adjust to the developments in technology and apply these to dental practice in communicating with dental professionals, medical doctors, dental technicians, and insurance providers, dental students need to be prepared to manage digitized data, ensure patient safety, and understand the advantages and limitations of conventional and digital processes.
Aim: To create awareness about digital teaching methodology among the dental students.
Materials and Methods: A cross-sectional survey was conducted among the adolescent population with a sample size of 120. A self administered structured questionnaire was prepared based on digital teaching methodology and consisted of 13 questions. It was circulated to participants through an online platform (google form). The statistics were done using SPSS software, chi-square test was used to check the association and P value of 0.05 was said to be statistically significant.The pros of the survey is that the adolescents of different lifestyles and cultures were surveyed. Children and adults were excluded from the survey. Simple random sampling method was the sampling method used to minimise the sampling bias.
Results: The results showed that the dental students are aware about the digital teaching methodology.
Conclusion: The people are aware of the digital teaching methodology. But more awareness needs to be spread so that digital handling can be improvised in the near future.
A Comparative Study of Low Versus Standard Intraperitoneal Pressures in Gynaecological Laparoscopic Surgery
Authors: Patil Anup R. , Dewani Deepika , Mahajan Kalyani , Acharya Neema , Jaiswal Arpita , Inamdar Saunitra A. , Madaan Sparsh 
Published: December 2021

Background: Minimal access surgery in contrast to open surgery has quicker recovery during the postoperative period as well as reduced scores of pain. As a result of increased pressure in the abdominal cavity, laparoscopic surgery has many implications over a range of organ systems as well as their functioning. Laparoscopic surgery due to increased intraabdominal pressure also has many implications on various organ systems and their functioning. To overcome the consequences of increased intrabdominal pressure, a number of trials have been formulated to compare low- versus standard-pressure pneumoperitoneum.
Aim: The aim of this study was to assess the effectivity of low intraperitoneal pressures v/s standard intraperitoneal pressure during laparoscopic hysterectomies.
Study Design: Experimental study
Materials and Methods: 40 cases with uncomplicated symptomatic benign uterine pathologies who were posted for laparoscopic hysterectomy were selected out of which 20-20 cases were randomized into low and standard pneumoperitoneum groups.
Results: In patients in whom low pressure pneumoperitoneum is employed are better recovered in terms of pain than standard pressure pneumoperitoneum. This means hospital stay can be shortened in low pressure pneumoperitoneum groups which will be more economical and comfortable for patients.
Conclusion: Laparoscopic hysterectomy can be done at 10 mmhg with the benefits of :

Optimum visualization with low pressure
Reduction in post operative pain helping the patient for early ambulation so that patient will get back to routine work and normal life earlier, it is the main purpose of minimal invasive surgery.
A Smart Semi‐Implantable Device Integrating Microchannel‐Enhanced Sampling and Multiplex Biochemical Testing for Deep Wound Monitoring and Pathogen Identification
Authors: Li Qilin , Wei Chunyu , Xu Luming , Zhang Jiao , Li Yuyu , Lu Xiaohuan , Xu Rengui , Guo Honglian , Cao Peng , Ouyang Chenke , Xu Jiarong , Chen Wei , Wang Zheng , Wang Lin 
Published: January 2025

AbstractMonitoring deep wounds is challenging but necessary for high‐quality medical treatment. Current methodologies for deep wound monitoring are typically limited to indirect clinical symptoms or costly non‐real‐time imaging diagnosis. Herein, a smart system is proposed that enables in situ monitoring of deep wounds’ status through a semi‐implantable device composed of 2 seamlessly connected functional components: 1) the well‐designed, microchannel‐structured sampling needles that efficiently and conveniently collect samples from deep wound anatomical locations, and 2) the multiplex biochemical testing compartment that facilitates the immediate and persistent detection of multiple biochemical indicators based on a color image processing software accessible to a conventional smartphone. With the 3 representative preclinical deep wound models, the study demonstrates the device's potential to monitor wound infection, inflammation, healing progress, and reduce inflammation when applied to deep skin injury, surgical implantation, and postoperative intestinal leakage. The device's capability to rapidly and accurately identify pathogenic bacteria is also demonstrated both in vitro and in vivo, potentially facilitating precise intervention in infected wounds. Coupled with the device's favorable biocompatibility and cost‐effectiveness, this intelligent system emerges as a promising tool for safe and effective management of complicated deep wounds.