bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidrug-Resistant Klebsiella Pneumoniae: A Retrospective Study in Manaus, Brazil
Authors: Nakamura-Silva Rafael , Cerdeira Louise , Oliveira-Silva Mariana , Costa Karen Regina Carim da , Sano Elder , Fuga Bruna , Moura Quézia , Esposito Fernanda , Lincopan Nilton , Wyres Kelly , Pitondo-Silva André 
Published: November 2021

Abstract
Klebsiella pneumoniae is an opportunistic pathogen that can cause several infections, mainly in hospitalised or immunocompromised individuals. The spread of K. pneumoniae emerging virulent and multidrug-resistant clones is a worldwide concern and its identification is crucial to control these strains especially in hospitals. This article reports data related to multi-resistant K. pneumoniae strains, isolated from inpatients in the city of Manaus, Brazil, harbouring virulence and antimicrobial resistance genes, including high-risk international clones belonging to clonal group (CG) 258. Twenty-one strains isolated from different patients admitted to four hospitals in the city of Manaus, located in the state of Amazonas, Northern Brazil (Amazon Rainforest region) were evaluated. The majority of strains (61.9 % n = 13) were classified as multidrug-resistant (MDR), and five strains (23.8 %) as extensively drug-resistant (XDR). Several virulence and antimicrobial resistance genes were found among the strains and eight strains (38.1 %) presented the hypermucoviscous phenotype. MLST analysis demonstrated a great diversity of STs among the strains, totaling 12 different STs (ST11, ST23, ST198, ST277, ST307, ST340, ST378, ST462, ST502, ST3991, ST3993 and ST5209). Four of these (ST11, ST23, ST307 and ST340) belong to CG258.
Skin Autofluorescence Profile in Tunisian Subjects with and Without Metabolic Disorders
Authors: Khochtali Ines , Bouida Wahid , Boubaker Hamdi , Nouira Semir , Kerkeni Mohsen 
Published: November 2021

Abstract
Background: The AGE Reader, as a clinical tool for non invasive assessment, measures the accumulation of advanced glycation end products (AGEs) in skin tissues shown as skin autofluorescence (SAF). AGEs Accumulation has been implicated in several diseases. There is no data about SAF profile in Tunisian population, this study aimed to assess firstly SAF profile in subjects with and without metabolic disorders and secondly to examine the association between SAF and various clinical parameters.Methods: In a cross-sectional study, we included 220 participants between 19 and 86 years of age who were subdivided in two groups: a healthy group (n=93) and patients group with metabolic disorders (n=127) contains three subgroups as following: diabetic patients (n=32), hypertensive patients (n=54) and patients with both diabetes and hypertension (n=41). Skin AGEs accumulation was measured by AGE Reader and clinical data was obtained.Results: SAF was significantly higher in patients group compared to healthy group [2.40 AU (2.10-2.60) vs. 2.00 AU (1.70-2.10) respectively; P <0.001]. Diabetic patients and hypertensive patients showed an increased level of SAF. The highest of SAF was observed in patient with both coexistence of diabetes, hypertension and dyslipidemia. SAF was associated with age, gender, BMI, duration of diabetes, HbA1c, triglyceride and obesity. Multivariate analysis showed that age and duration of diabetes were independent determinant of SAF. The ROC analysis indicated that an SAF > 2.25 AU was optimal cut-off point to predict the presence of metabolic disorders (P <0.001). Conclusion: SAF was increased in patients with diabetes and/or with hypertension and dyslipidemia. AGE Reader device is a rapid and helpful tool in clinical practice for evaluating and screening metabolic disorders in undiagnosed subjects.
Effect of Impurities on a Two-Dimensional Dodecagonal Quasicrystal
Authors: Fuwa Masahiro , Sato Masahide 
Published: November 2021

Abstract
Langevin dynamics simulations are performed to examine how impurities affect two-dimensional dodecagonal quasicrystals. We assumed that the interaction potential between two particles is the Lennard-Jones-Gauss potential if at least one of these particles is a matrix particle and that the interaction potential between two impurities is the Lennard-Jones potential. Matrix particles and impurities impinge with constant rates on the substrate created by a part of a dodecagonal quasicrystal consisting of square and triangular tiles. The dependences of the twelve-fold rotational order and the number of shield-like tiles on the impurity density are examined after sufficient solid layers are grown. While the change in the twelve-fold rotational symmetry is small, the number of shield-like tiles in the solid increases greatly with increasing impurity density.
The Involvement of CXCL17-GPR35 in Gastric Cancer Initiation and Development
Authors: Li Yi-zhi , Liu Ao-ran , Liu Song-yi , Yan Li-rong , Yuan Yuan , Xu Qian 
Published: November 2021

Abstract
Background: C-X-C motif chemokine 17 (CXCL17), a newly identified chemokine, and its possible receptor, G-protein coupled receptor 35 (GPR35) were potential proteins involved in carcinogenesis; nevertheless, their involvement in gastric cancer (GC) initiation and development, which we aimed to evaluated in this study, remains undefined.Methods and materials: CXCL17 and GPR35 expressions in normal gastric gland of non-atrophic gastritis (NAG-NOR), intestinal metaplasia of atrophic gastritis (AG-IM), IM adjacent to GC (GC-IM), and GC tissues was determined by immunohistochemistry. Their possible roles were analyzed by the Cancer Genome Atlas (TCGA) data mining and western blot.Results: Either CXCL17 or GPR35 showed a strongest expression in GC-IM among the four types of tissues: CXCL17: GC-IM vs. AG-IM (P < 0.001), GC-IM vs. GC (P < 0.001); GPR35: GC-IM vs. AG-IM (P < 0.001), GC-IM vs. GC (P < 0.01). Their expressions were positively correlated in GC tissues (Spearman r = 0.2933, P < 0.001). CXCL17 expression was negatively correlated with the depth of tumor invasion (P = 0.015) and tumor maximum diameter (P = 0.003). Higher CXCL17 expression independently predicted for better survival in GC patients. TCGA analysis suggested CXCL17 expression was positively correlated with the transcriptional level of gastric mucosal maturation indicators TFF1, TFF2, PGC and MUC5AC, but negatively with the dedifferentiation indicators CDX1 and SFRP family members. Protein-protein interaction (PPI) revealed the potential connection of GPR35-CXCL17-CCL20. Overexpressing CXCL17 in HGC27 cell line downregulated SFRP2 and CDX1, but upregulated CCL20 expression.Conclusion: CXCL17 and GPR35 may play a vital role in GC initiation and development, especially in the IM to GC process. A higher CXCL17 expression independently predicted for better survival in GC patients, with a possible role in maintaining gastric mucosa maturation.
Enzyme-catalyzed Synthesis of Novel Solid–liquid Phase Change Energy Storage Materials Based on Levulinic Acid and 1,4 butanediol
Authors: Zhai Siyu , Zhang Lihe , Zhao Xi , Wang Qian , Yan Yin , Li Cui , Xu Zhang 
Published: November 2021

Abstract
With the rapid development of society, all kinds of non-renewable energy resources are constantly developed and utilized, energy storage is one of the best ways to solve the energy shortage. In this study , levulinic acid (LA) and 1,4 butanediol (BDO) were used to synthesize a novel polyol ester (LABDO) by biological and chemical methods. The biological method exhibited excellent performance in the synthesis process, where 87.5% of LABDO yield under optimal conditions, while the chemical method had more byproducts and higher energy consumption. Finally, the thermal properties of the obtained phase change materials (PCMs) were evaluated. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) showed that the melting temperature of LABDO was 50.51°C, the latent heat of melting was 156.1J/g, and the pyrolysis temperature was 150-160°C. Compared with traditional paraffin wax, the prepared PCMs have suitable phase transition temperature, higher latent heat of melting and better thermal stability. The thermal conductivity can be increased to 0.34W·m-1· k-1 by adding expanded graphite. In summary, LABDO can be used as low temperature phase change energy storage materials.
Comparative transcriptomics identifies the transcription factors BRANCHED1 and TCP4, as well as the microRNA miR166 as candidate genes involved in the evolutionary transition from dehiscent to indehiscent fruits in Lepidium
Authors: Gramzow Lydia , Klupsch Katharina , Pozo Noé Fernández , Hölzer Martin , Marz Manja , Rensing Stefan A. , Theißen Günter 
Published: November 2021

Abstract
Background: Fruits are the seed-bearing structures of flowering plants and are highly diverse in terms of morphology, texture and maturation. Dehiscent fruits split open upon maturation to discharge their seeds while indehiscent fruits are dispersed as a whole. Indehiscent fruits evolved from dehiscent fruits several times independently in the crucifer family (Brassicaceae). The fruits of Lepidium appelianum, for example, are indehiscent while the fruits of the closely related L. campestre are dehiscent. Here, we investigate the molecular and genetic mechanisms underlying the evolutionary transition from dehiscent to indehiscent fruits using these two Lepidium species as model system.Results: We have sequenced the transcriptomes and small RNAs of floral buds, flowers and fruits of L. appelianum and L. campestre and analyzed differentially expressed genes (DEGs) and differently differentially expressed genes (DDEGs). DEGs are genes that show significantly different transcript levels in the same structures (buds, flowers and fruits) in different species, or in different structures in the same species. DDEGs are genes for which the change in expression level between two structures is significantly different in one species than in the other. Comparing the two species, the highest number of DEGs was found in flowers, followed by fruits and floral buds while the highest number of DDEGs was found in fruits versus flowers followed by flowers versus floral buds. Several gene ontology terms related to cell wall synthesis and degradation were overrepresented in different sets of DEGs highlighting the importance of these processes for fruit opening. Furthermore, the fruit valve identity genes FRUITFUL and YABBY3 were among the DEGs identified. Finally, the microRNA miR166 as well as the TCP transcription factors BRANCHED1 (BRC1) and TCP FAMILY TRANSCRIPTION FACTOR 4 (TCP4) were found to be DDEGs.Conclusions: Our study reveals differences in gene expression between dehiscent and indehiscent fruits and uncovers miR166, BRC1 and TCP4 as possible causes for the evolutionary transition from dehiscent to indehiscent fruits in Lepidium.
Resonant Frequency of Circular Sectorial Microstrip Antenna Printed On Double-Layered Anisotropic Substrates
Authors: FRIHA Souad , Bedra Sami , CHEBBARA Fouad , BEDRA Randa , BENKOUDA Siham 
Published: November 2021

Abstract
In this work, modal characteristics have been rigorously studied which germinate an improved, accurate, and efficient computer-aided design (CAD) formulation to estimate the resonant frequency of sectorial circular microstrip antennas printed on anisotropic suspended and composite substrates. The obtained results demonstrated that the resonant frequencies of the sectorial circular microstrip patch on suspended and composite substrates can be adjusted to obtain the maximum operating frequency of the antenna. The computed results show a fairly good agreement with measured results. Such theoretical validation and results may prove to be more useful for design engineers and further investigation.
Movement Patterns and Habitat use of Adult Giant Trevally (Caranx Ignobilis) in the South China Sea
Authors: Chiang Wei‐Chuan , Lin Shian-Jhong , Soong Ker-Yea , Liao Te-Yu , Chen Yu-Yun , Ho Yuan-Hsing , Tseng Chen-Te , Musyl Michael K. 
Published: November 2021

Abstract
This paper presents the first application of pop-up satellite archival tags (PSATs) to giant trevally (Caranx ignobilis) in a "no-take" marine protected area (MPA) comprising in a major reef system in the South China Sea (Dongsha Attol National Park). In order to determine appropriate management strategies in an around the MPA and to delimit stock boundaries to help restore populations, fundamental ecological information is required on movement patterns, habitat preferences and home range. In total 10 giant trevally were tagged from May 2016 to September 2018. Eight tags reported and remained affixed from 17 to 243 days and linear displacements ranged from 26 to 826 km from deployment to pop-up locations. Fish were mainly confined to the mixed-layer but occasionally made deeper descents (~50 -60 m) during nighttime and the distributions of time spent at depth (~0 - 67 m) and temperature (21.5 - 35.4°C) were significantly different between daytime and nighttime but the transitions were not pronounced. Most probable tracks calculated from a state-space Kalman filter suggested site-fidelity and/or cyclic N-S dispersal patterns possibly related to spawning or foraging as about half of the pop-up locations were within ~100 km of the tagging location. Given these findings and implications, it is possible that giant trevally may need to be managed at small spatial scales to preserve genetic diversity. Additional tagging studies, however, using genetic data and conventional tags to maximize cost-benefit and augmented with a sub-set of PSATs, will be required to test this hypothesis at a higher level of statistical power.
LncRNA PVT1 Regulates Biological Function of Osteoarthritis Cells by Regulating miR-497/AKT3 Axis
Authors: Chen Peng , Zhang Ying 
Published: November 2021

Abstract
Objective

There is growing evidence that lncRNA plays a role in the progression of many diseases, including osteoarthritis (OA). However, the role of lncRNA PVT1 in OA is still unclear. Thus, the present study aims to explore the function of PVT1 in the OA process, and analyze the possible mechanisms thereof.
Methods

PVT1 expression in articular cartilage tissue of OA patients and non-OA patients was evaluated. PVT1, miR-497 and AKT3 in IL-1β induced chondrocytes were knocked down or elevated to determine the proliferation and apoptosis of treated chondrocytes. And the changes of AKT3 and extracellular matrix (ECM) related factors (aggrecan, collagen Type II and MMP-9) were detected.
Results

PVT1 was elevated in OA cartilage tissue and chondrocytes induced by IL-1. Cell proliferation ability was enhanced while apoptosis rate was declined after PVT1 knock down and miR-497 elevation. In addition, aggrecan and collagen Type II levels reduced in IL-1β induced chondrocytes, while MMP-9 level elevated. Dual luciferase reporter confirmed that PVT1 and AKT3 interacted with miR-497, respectively. Rescue experiments revealed that increasing AKT3 or knocking down miR-497 could reverse the impacts of knocking down PVT1 on IL-1β-induced chondrocytes.
Conclusion

PVT1 is up-regulated in OA, and knock down PVT1 can decrease the effects of IL-1βon proliferation, apoptosis, and ECM degradation of chondrocyte through regulating miR-497/AKT3 axis.
Synthesis and characterization of Ni2C: An efficient electrocatalyst towards hydrogen evolution reaction
Authors: Vilvanathan Sathish , Gunasekaran Roshini 
Published: November 2021

Abstract
Ni2C electrocatalyst were synthesized and characterized for the Hydrogen Evolution Reaction (HER) electrolyzer. One step hydrothermal technique is used to synthesize Ni2C sample. Platinum based electro catalyst materials are initial and best electro catalyst for Hydrogen Evolution Reaction (HER). Ni2C (Nickel Carbide electro catalyst) was examined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD). Electrochemical characterization like cyclic voltammetry (CV), Tafel polarization and electrochemical impedance spectroscopy (EIS) studies is employed to explore the elctrocatalytic behavior of Ni2C material for HER. FTIR study confirms the presence of Ni2C electrocatalyst by the presence of metal peaks and various functional groups. The isomeric nature and purity of synthesized material were explored by powder X-ray diffraction studies. Cyclic voltammetry technique was performed in 0.5 M H2SO4 solution to attain the polarization curve of Ni2C electrocatalyst for HER.
Music intervention affects the quality of life on Alzheimer’s disease: a meta-analysis
Authors: Yu Xin 
Published: November 2021

Abstract
BackgroundMusic intervention has been found to be beneficial for neurological diseases, especially in slowing down the progression of clinical symptom in patients. However, it remains unclear to what extend can music intervention may affect patients with Alzheimer’s disease (AD). Questions remain on whether music intervention can improve the quality of life (QOL) of AD patients, who are usually with poor QOL owning to the burden of disease.MethodsA search that compared the effectiveness of music intervention to improve the QOL of AD patients were carried out. PubMed, Cochrane library clinical trial database, and WANFANG database were searched to collect data from randomized controlled trials (RCTs). Two independent researchers extracted data from the selected eligible articles by using collection form and RevMan5.3 statistical software and applied for the meta-analysis.ResultsWe included six RCTs in our meta-analysis. The results showed that compared to the control group, music intervention can significantly increase the score of the QOL-AD scale (MD=5.10, 95%CI: 2.95-7.24, P<0.001) and The World Health Organization QOL Rating Scale Short Form score (MD=5.76, 95%CI: 1.59-9.92, P=0.007). ConclusionsResults suggested that music intervention could improve the QOL for the elderly patients with AD. Our findings indicated that music intervention might be considered as a non-pharmaceutical therapy for patients with AD in the future.
Efficacy and Safety of Elian Granules in Treating Chronic Atrophic Gastritis: Study Protocol For a Randomized, Double-Blind, Placebo-Controlled, Multicenter Clinical Trial
Authors: Gu Zhijian , Cong Jun , Gong Biao , Cen Rong , Chen Yongqi , Jia Qingling , Wu Chenheng , Ling Jianghong , Tang Xudong 
Published: November 2021

Abstract
Background: Multifocal atrophic gastritis and intestinal metaplasia are considered to be important links of the gastric precancerous cascade. But, there is a lack of definite therapeutic drugs for them. Many studies have shown traditional Chinese medicine is effective and no serious side effects have been identified. However, the studies that have been carried out were not scientifically rigorous trials. Our aim is to design a high-quality trial for a Chinese patent medicine, Elian granules, to investigate the efficacy and safety of this drug in treating chronic atrophic gastritis patients with or without intestinal metaplasia.Methods: This is a phase Ⅱ, randomized, double-blind, placebo-controlled, multicenter clinical trial. A total of 240 participants will be assigned to treatment group or placebo control group with a 1:1 ratio. Then, the experimental drug or placebo will be taken with boiling water,2 small bags (24.2g) each time, twice times a day, half an hour after each meal for 24 weeks. The primary outcome is to observe gastric mucosal histological changes after 6 months in patients with atrophic gastritis with or without intestinal metaplasia based on OLGA/OLGIM. The secondary outcome included dyspepsia symptom score and quality of life scale.Discussion: This study is designed to evaluate the efficacy and safety of Elian granule in a randomized, double-blind, placebo-controlled, multicenter manner. This trial may not only provide evidence for a phase III clinical trial, but also a vision of an alternative option for chronic atrophic gastritis(CAG) treatment.Trial registration: The registration number, ChiMCTR2000003929, was assigned by the Registry Platform For Evidence Based Traditional Chinese Medicine on 13 September 2020.
A GIS Based Morphometric Analysis of Watershed for Soil Erosion Planning
Authors: Wagh Santosh , Manekar Vivek 
Published: November 2021

Abstract
Soil erosion, if remain non attentive, will increase the sediment load of the river and also affecting the life of the hydraulic structures constructed across it. Hence, proper investigations related to the soil erosion is very much essential for watershed planners and designers. In the present study, morphometric analysis is carried out for basic, linear, areal, shape and landscape aspects using 28 morphometric parameters for Bhima river watershed to prioritize and categorized it based on its erosive potential. Total 48 toposheet of Survey of India of the scale 1:50,000 are used to delineate the watersheds for the preparation of base map containing information about drainage, contours, etc. so as to ensure accuracy and quality of the work. The All India Soil and Land Use Survey (AISLUS) codification is adopted for the study area. According to AISLUS, the study area falls under region 4, covered in 19 watersheds. Morphometric parameters in Arc-GIS software and compound factor method is employed to identify the sub-watersheds which are susceptible to soil erosion. Final Priority Ranking (FPR) based category map of watersheds is reported in this study by categorized it under five categories indicating % area of each category (very high category: 15.94%; high category: 23.50%; medium category: 12.73%; low category: 23.90%; and very low category: 23.93%). Based on the findings, this study is suggesting suitable sites soil conservation practices for reducing the sediment load in Bhima river watersheds as well as Ujjani reservoir, which will be useful to the concerning authorities for better management.
Credentialed Contrarians: The Misrepresentation of Climate Change Expertise in Congress
Authors: Lee Nathan , Yeganeh Sara , Suhay Elizabeth , Nash Erin 
Published: November 2021

Abstract
Does partisan politics distort the representation of climate change expertise in U.S. policymaking? To examine this question, we classify each Congressional witness who testified on climate change between 1997 and 2016 in terms of both their expert credentials and their climate change beliefs. In contrast to prevailing wisdom, we find that Republicans are slightly more likely to call credentialed experts than Democrats. However, this pattern is largely driven by the presence of credentialed contrarians, i.e., experts who have contradicted widely accepted conclusions of their own community. Although these individuals represent less than one percent of climate change experts outside of Congress, they account for over a quarter of expert testimonies in Congress. More generally, these findings illustrate how politicians at odds with the expert community can strategically and repeatedly select the small minority of individuals who both lend credibility to their political views and meet shared standards of professionalized expertise.