bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ap2s1 mutation causes hypercalcaemia in mice and impairs interaction between calcium-sensing receptor and adaptor protein-2
Authors: Hannan Fadil M , Stevenson Mark , Bayliss Asha L , Stokes Victoria J , Stewart Michelle , Kooblall Kreepa G , Gorvin Caroline M , Codner Gemma , Teboul Lydia , Wells Sara , Thakker Rajesh V 
Published: March 2021

Abstract
Adaptor protein 2 (AP2), a heterotetrameric complex comprising AP2α, AP2β2, AP2μ2 and AP2σ2 subunits, is ubiquitously expressed and involved in endocytosis and trafficking of membrane proteins, such as the calcium-sensing receptor (CaSR), a G-protein coupled receptor that signals via Gα11. Mutations of CaSR, Gα11 and AP2σ2, encoded by AP2S1, cause familial hypocalciuric hypercalcaemia types 1–3 (FHH1–3), respectively. FHH3 patients have heterozygous AP2S1 missense Arg15 mutations (p.Arg15Cys, p.Arg15His or p.Arg15Leu) with hypercalcaemia, which may be marked and symptomatic, and occasional hypophosphataemia and osteomalacia. To further characterize the phenotypic spectrum and calcitropic pathophysiology of FHH3, we used CRISPR/Cas9 genome editing to generate mice harboring the AP2S1 p.Arg15Leu mutation, which causes the most severe FHH3 phenotype. Heterozygous (Ap2s1+/L15) mice were viable, and had marked hypercalcaemia, hypermagnesaemia, hypophosphataemia, and increases in alkaline phosphatase activity and fibroblast growth factor-23. Plasma 1,25-dihydroxyvitamin D was normal, and no alterations in bone mineral density or bone turnover were noted. Homozygous (Ap2s1L15/L15) mice invariably died perinatally. Co-immunoprecipitation studies showed that the AP2S1 p.Arg15Leu mutation impaired protein–protein interactions between AP2σ2 and the other AP2 subunits, and also with the CaSR. Cinacalcet, a CaSR positive allosteric modulator, decreased plasma calcium and parathyroid hormone concentrations in Ap2s1+/L15 mice, but had no effect on the diminished AP2σ2-CaSR interaction in vitro. Thus, our studies have established a mouse model that is representative for FHH3 in humans, and demonstrated that the AP2S1 p.Arg15Leu mutation causes a predominantly calcitropic phenotype, which can be ameliorated by treatment with cinacalcet.
The party scene: new directions for political party research in foreign policy analysis
Authors: Hofmann Stephanie C , Martill Benjamin 
Published: March 2021

Abstract
Research on political parties and foreign policy has grown in recent years in response to disciplinary and real-world changes. But party research still bears the imprint of earlier scepticism about the role of parties. The result is scholarship which is disaggregated, which avoids difficult cases for parties, and which has focused more on showing that parties matter relative to structural accounts of foreign policy-making. This article takes stock of recent research on political parties, party politics and their role in foreign policy-making. We argue that it is time for party research not only to embrace the question of whether parties matter but also how, when and where they matter. This requires a move away from most-likely cases and the realist foil towards an embrace of the complexity of party positions. Building on International Relations, comparative politics and foreign policy analysis scholarship, we suggest four avenues deserving of greater scholarly focus: 1) ideological multidimensionality; 2) parties as organizations and the role of entrepreneurs; 3) parties as transnational foreign policy actors; and 4) the interaction between parties and the changing global order. We propose how these literatures can help identify new research questions, contribute to theory development and help define scope conditions. This will hopefully help scholars establish benchmarks for judging the efficacy of parties in foreign policy-making.
Trade specialization and performance in global value chains
Authors: Bontadini Filippo 
Published: March 2021

Abstract
This article investigates whether trade specialization explains economies’ trade performance within the context of global value chains (GVCs). We consider trade specialization in natural resources (NR), high- and low-tech manufacturing (LTM and HTM) and business services, before and after the financial crisis. The aimed contribution of this article is to shed light on the effects of trade specialization as measured in domestic value added embodied in exports rather than gross exports. We add to the literature on GVCs by (i) studying the role of the domestic productive structure in countries’ trade specialization and performance, (ii) accounting for the rate of changes in trade specialization as affecting GVC performance. We employ Balassa indexes based on value added flows in a GMM dynamic panel framework. Our results suggest that trade specialization in LTM and NR relate to a decline in the growth of domestic value-added per capita exports relative to other countries. HTM and knowledge-intensive business services exhibit a positive effect during the crisis period. We discuss these findings in relation to the recent debates on the role of manufacturing and premature de-industrialization in developing countries.
Physicians’ perceptions of their knowledge and the preparedness of health facilities in Angola to diagnose and manage COVID-19
Authors: Arrais Margarete , Dias Welwitschia , Gama Jorge M R , Brito Miguel 
Published: March 2021

Abstract

Background
Healthcare professionals represent a vulnerable group in terms of responding to COVID-19. Knowledge can influence healthcare professionals through adoption of the correct attitudes and practices. The aim of this study was to evaluate, by a questionnaire, the perceptions of physicians about their level of knowledge as well as conditions in their workplaces for dealing with COVID-19.

Methods
A cross-sectional study of Angolan physicians took place from 11 May to 23 June 2020. A questionnaire was electronically shared across social media and via email.

Results
The sample consisted of 637 valid questionnaires; 53% of respondents were female, 41% were aged 31–40 y and 79% were from Luanda province. About 51% of physicians perceived that they had adequate knowledge about COVID-19 and 64% used personal protective equipment. These figures were higher among specialists from the province of Luanda. About 81% stated that their health units lacked the technical capacity to diagnose COVID-19. Only 35% of health units have chest tomography equipment; 44% are prepared for the care and/or isolation of patients. Only 33% of units are running intensive care units. The main concerns of physicians were training opportunities and limited access to personal protective equipment.

Conclusion
The results show that it is necessary to strengthen physicians’ knowledge about COVID-19, as well as to improve the conditions of the health units, so as to promote safe practices.
Retention of Human Body Fluids in Adults ofCalliphora vicina(Diptera: Calliphoridae)
Authors: Rivers David B , Hammerschmidt Claire , Carrigan Alexandra , Melvin Kayleen 
Published: March 2021

AbstractForaging by Calliphora vicina Robineau-Desvoidy often leads to a period of bubbling behavior, followed by either deposition of the regurgitate onto surfaces or reuptake of the bubble. Eventually, the partially or undigested food is passed in the excreta forming fecal or defecatory stains on surfaces in which deposition occurs. This study examined the digestive artifacts (i.e., regurgitate and defecatory stains) formed following consumption of human blood and semen by adult flies in an attempt to determine the length of time the meal was retained in the crop. The morphological appearance of either type of stain appeared consistent with the color of blood or semen for 10–20 d after feeding. When tested with ABA Hematrace immunochromatographic strip assays, blood was detectable in at least 33% of fly artifacts 25 d after the initial consumption of blood. Similarly, semen was detected in nearly 34% of digestive artifacts 30 d after feeding on human semen when using ABA p30 cards. Human body fluids were also detected in fly artifacts when using RSID lateral flow assays, but a much lower percentage of artifacts tested positive for blood (4.9%) and semen (4.6%) 25-d postfeeding in comparison to ABA strip assays. The difference between the types of lateral flow assays appeared to be due to extraction efficiencies of the buffers used for isolation of blood or semen from the fly artifacts. The implications of these observations in reference to seasonal adaptations and to bloodstain pattern analysis at crime scenes are discussed.
Dynamics of axially functionally graded conical pipes conveying fluid
Authors: Zhao Yuzhen , Hu Dike , Wu Song , Long Xinjun , Liu Yongshou 
Published: March 2021

AbstractIn this paper, the dynamics of axially functionally graded (AFG) conical pipes conveying fluid are analyzed. The materials are distributed along the conical pipe axis as a volume fraction function. Either the elastic modulus or the density of the AFG conical pipe is assumed to vary from the inlet to the outlet. The governing equation of the AFG conical pipe is derived using the Hamiltonian principle and solved by the differential quadrature method. The effects of the volume fraction index, volume fraction function type and reduction factor on the natural frequency and critical velocity are analyzed. It is found that for a power function volume fraction type, the natural frequency and critical velocity increase with increasing volume fraction index and clearly increase when the volume fraction index is within the range (0, 10). For an exponential function volume fraction type, the natural frequency and critical velocity change rapidly within the range (−10, 10), besides the above range the relationship between the natural frequency, critical velocity and volume fraction index is approximate of little change. The natural frequency and critical velocity decrease linearly with increasing reduction factor.
The spatially resolved gas and dust connection in neutral inflows and outflows in nearby AGN
Authors: Rupke David S N , Thomas Adam D , Dopita Michael A 
Published: March 2021

ABSTRACT
Dusty, neutral outflows and inflows are a common feature of nearby star-forming galaxies. We characterize these flows in eight galaxies – mostly active galactic nuclei (AGN) – selected for their widespread Na i D signatures from the Siding Spring Southern Seyfert Spectroscopic Snapshot Survey (S7). This survey employs deep, wide field-of-view (FOV) integral field spectroscopy at moderate spectral resolution (R = 7000 at Na i D). We significantly expand the sample of sightlines in external galaxies in which the spatially resolved relationship has been studied between cool, neutral gas properties – N(Na i), Weq(Na i D) – and dust – E(B − V) from both stars and gas. Our sample shows strong, significant correlations of total Weq with E(B − V)⋆ and g − i colour within individual galaxies; correlations with E(B − V)gas are present but weaker. Regressions yield slope variations from galaxy to galaxy and intrinsic scatter ∼1 Å. The sample occupies regions in the space of N(Na i) and $W_\mathrm{eq}^\mathrm{abs}$ versus E(B − V)gas that are consistent with extrapolations from other studies to higher colour excess [E(B − V)gas ∼ 1]. For perhaps the first time in external galaxies, we detect inverse P Cygni profiles in the Na i D line, presumably due to inflowing gas. Via Doppler-shifted Na i D absorption and emission lines, we find ubiquitous flows that differ from stellar rotation by $\gtrsim$100 km s−1 or have $|v_{\mathrm{ abs}} - v_{\mathrm{ em}}|\gtrsim 100$ km s−1. Inflows and outflows extend towards the edge of the detected stellar disc/FOV, together subtend 10–40 per cent of the projected disc, and have similar mean N(Na i) and Weq(Na i D). Outflows are consistent with minor axis or jet-driven flows, while inflows tend towards the projected major axis. The inflows may result from non-axisymmetric potentials, tidal motions, or halo infall.
The non-linear infrared-radio correlation of low-z galaxies: implications for redshift evolution, a new radio SFR recipe, and how to minimize selection bias
Authors: Molnár Dániel Cs , Sargent Mark T , Leslie Sarah , Magnelli Benjamin , Schinnerer Eva , Zamorani Giovanni , Delhaize Jacinta , Smolčić Vernesa , Tisanić Krešimir , Vardoulaki Eleni 
Published: March 2021

ABSTRACT
The infrared-radio correlation (IRRC) underpins many commonly used radio luminosity–star formation rate (SFR) calibrations. In preparation for the new generation of radio surveys, we revisit the IRRC of low-z galaxies by (a) drawing on the best currently available infrared (IR) and 1.4 GHz radio photometry, plus ancillary data over the widest possible area, and (b) carefully assessing potential systematics. We compile a catalogue of ∼9500, z &lt; 0.2 galaxies and derive their 1.4 GHz radio (L1.4), total IR, and monochromatic IR luminosities in up to seven bands, allowing us to parametrize the wavelength dependence of monochromatic IRRCs from 22–500 µm. For the first time for low-z samples, we quantify how poorly matched IR and radio survey depths bias measured median IR/radio ratios, $\overline{q}_{\mathrm{TIR}}$, and discuss the level of biasing expected for low-z IRRC studies in ASKAP/MeerKAT fields. For our subset of ∼2000 high-confidence star-forming galaxies, we find a median $\overline{q}_{\mathrm{TIR}}$ of 2.54 (scatter: 0.17 dex). We show that $\overline{q}_{\mathrm{TIR}}$ correlates with L1.4, implying a non-linear IRRC with slope 1.11 ± 0.01. Our new L1.4–SFR calibration, which incorporates this non-linearity, reproduces SFRs from panchromatic SED fits substantially better than previous IRRC-based recipes. Finally, we match the evolutionary slope of recently measured $\overline{q}_{\mathrm{TIR}}$–redshift trends without having to invoke redshift evolution of the IRRC. In this framework, the redshift evolution of $\overline{q}_{\mathrm{TIR}}$ reported at GHz frequencies in the literature is the consequence of a partial, redshift-dependent sampling of a non-linear IRRC obeyed by low-z and distant galaxies.
Convective core entrainment in 1D main-sequence stellar models
Authors: Scott L J A , Hirschi R , Georgy C , Arnett W D , Meakin C , Kaiser E A , Ekström S , Yusof N 
Published: March 2021

ABSTRACT
3D hydrodynamics models of deep stellar convection exhibit turbulent entrainment at the convective-radiative boundary which follows the entrainment law, varying with boundary penetrability. We implement the entrainment law in the 1D Geneva stellar evolution code. We then calculate models between 1.5 and 60 M⊙ at solar metallicity (Z = 0.014) and compare them to previous generations of models and observations on the main sequence. The boundary penetrability, quantified by the bulk Richardson number, RiB, varies with mass and to a smaller extent with time. The variation of RiB with mass is due to the mass dependence of typical convective velocities in the core and hence the luminosity of the star. The chemical gradient above the convective core dominates the variation of RiB with time. An entrainment law method can therefore explain the apparent mass dependence of convective boundary mixing through RiB. New models including entrainment can better reproduce the mass dependence of the main-sequence width using entrainment law parameters A ∼ 2 × 10−4 and n = 1. We compare these empirically constrained values to the results of 3D hydrodynamics simulations and discuss implications.
Segregated brain state during hypnosis
Authors: Tuominen Jarno , Kallio Sakari , Kaasinen Valtteri , Railo Henry 
Published: March 2021

Abstract
Can the brain be shifted into a different state using a simple social cue, as tests on highly hypnotizable subjects would suggest? Demonstrating an altered global brain state is difficult. Brain activation varies greatly during wakefulness and can be voluntarily influenced. We measured the complexity of electrophysiological response to transcranial magnetic stimulation in one ‘hypnotic virtuoso’. Such a measure produces a response arguably outside the subject’s voluntary control and has been proven adequate for discriminating conscious from unconscious brain states. We show that a single-word hypnotic induction robustly shifted global neural connectivity into a state where activity remained sustained but failed to ignite strong, coherent activity in frontoparietal cortices. Changes in perturbational complexity indicate a similar move towards a more segregated state. We interpret these findings to suggest a shift in the underlying state of the brain, likely moderating subsequent hypnotic responding.
Integrative molecular characterization of pediatric spinal ependymoma: the UK Children’s Cancer and Leukaemia Group study
Authors: Ahmad Omar , Chapman Rebecca , Storer Lisa C , Luo Li , Heath Paul R , Resar Linda , Cohen Kenneth J , Grundy Richard G , Lourdusamy Anbarasu 
Published: March 2021

AbstractBackgroundPediatric spinal ependymomas (SP-EPNs) are rare primary central nervous system tumors with heterogeneous clinical course. Considering that ependymomas in children are biologically distinct from their adult counterparts, this study aimed to define the molecular landscape of SP-EPNs in children.MethodsIn this retrospective study, we have collected tumor samples from 27 SP-EPN patients younger than 18 years and carried out the histological review, DNA methylation, and gene expression profiling.ResultsUnsupervised analyses with methylation profiles revealed 2 subgroups where all grade I tumors (n = 11) were in Group 1, but the grade II/III tumors split into 2 groups (n = 7 in Group 1 and n = 9 in Group 2). The Heidelberg classifier assigned Group 1 tumors as spinal myxopapillary ependymomas (SP-MPEs), 5 Group 2 tumors as SP-EPNs, and failed to classify 4 Group 2 tumors. Copy numbers derived from DNA methylation arrays revealed subgroup-specific genetic alterations and showed that SP-EPN tumors lack MYCN amplification. Gene expression profiling revealed distinct transcriptomic signatures, including overexpression of genes involved in oxidative phosphorylation in SP-MPEs that were validated by Western blot analysis. We discovered widespread decreases in DNA methylation at enhancer regions that are associated with the expression of oncogenic signaling pathways in SP-MPEs. Furthermore, transcription factor motifs for master regulators, including HNF1B, PAX3, and ZIC3, were significantly overrepresented in probes specific to distal regulatory regions in SP-MPEs.ConclusionOur findings show substantial heterogeneity in pediatric SP-EPN and uncover novel enhancers and transcriptional pathways specific to the SP-MPE subgroup, providing a foundation for future therapeutic strategies.
Combined 18F-FET PET and diffusion kurtosis MRI in posttreatment glioblastoma: differentiation of true progression from treatment-related changes
Authors: D’Amore Francesco , Grinberg Farida , Mauler Jörg , Galldiks Norbert , Blazhenets Ganna , Farrher Ezequiel , Filss Christian , Stoffels Gabriele , Mottaghy Felix M , Lohmann Philipp , Shah Nadim Jon , Langen Karl-Josef 
Published: March 2021

Abstract

Background
Radiological differentiation of tumor progression (TPR) from treatment-related changes (TRC) in pretreated glioblastoma is crucial. This study aimed to explore the diagnostic value of diffusion kurtosis MRI combined with information derived from O-(2-[18F]-fluoroethyl)-l-tyrosine (18F-FET) PET for the differentiation of TPR from TRC in patients with pretreated glioblastoma.

Methods
Thirty-two patients with histomolecularly defined and pretreated glioblastoma suspected of having TPR were included in this retrospective study. Twenty-one patients were included in the TPR group, and 11 patients in the TRC group, as assessed by neuropathology or clinicoradiological follow-up. Three-dimensional (3D) regions of interest were generated based on increased 18F-FET uptake using a tumor-to-brain ratio of 1.6. Furthermore, diffusion MRI kurtosis maps were obtained from the same regions of interest using co-registered 18F-FET PET images, and advanced histogram analysis of diffusion kurtosis map parameters was applied to generated 3D regions of interest. Diagnostic accuracy was analyzed by receiver operating characteristic curve analysis and combinations of PET and MRI parameters using multivariate logistic regression.

Results
Parameters derived from diffusion MRI kurtosis maps show high diagnostic accuracy, up to 88%, for differentiating between TPR and TRC. Logistic regression revealed that the highest diagnostic accuracy of 94% (area under the curve, 0.97; sensitivity, 94%; specificity, 91%) was achieved by combining the maximum tumor-to-brain ratio of 18F-FET uptake and diffusion MRI kurtosis metrics.

Conclusions
The combined use of 18F-FET PET and MRI diffusion kurtosis maps appears to be a promising approach to improve the differentiation of TPR from TRC in pretreated glioblastoma and warrants further investigation.
Fiscal policy uncertainty and its effects on the real economy: German evidence
Authors: Beckmann Joscha , Czudaj Robert L 
Published: March 2021

AbstractThis article introduces a new measure of fiscal policy uncertainty (FPU) based on the disagreement among professional forecasters. We analyse different patterns of this measure for the German economy and also use Italian data for comparison. Especially, we examine the impact of the introduction of the German ‘debt brake’ on FPU. Finally, we conduct an impulse response analysis to investigate the effect of FPU on the real economy and we provide robust evidence that FPU significantly decreases the growth rate of industrial production. The corresponding effect is robust to various sensitivity checks and exceeds the impact of a general measure of economic policy uncertainty. In general, the negative effect on the real economy might be explained by lower hiring and investment by firms, higher costs of financing due to risk premia and lower consumption spending as a result of precautionary savings.
Supplemental Nutrition Assistance Program Eligibility and HIV Incidence in the United States
Authors: Richterman Aaron , Block Jason P , Tsai Alexander C , Ivers Louise C 
Published: March 2021

AbstractBackgroundThe connection between food insecurity and HIV outcomes is well established. The Supplemental Nutrition Assistance Program (SNAP), the primary food safety net program in the United States, may have collateral impacts on HIV incidence. “Broad-based categorical eligibility” for SNAP is a policy that provides a mechanism for states to increase the income or asset limits for SNAP eligibility.MethodsWe estimated the association between the number of new HIV diagnoses in 2010–2014 for each state and (1) state income limits and (2) state asset limits for SNAP eligibility. We fitted multivariable negative binomial regression models with number of HIV diagnoses specified as the outcome; SNAP policies as the primary explanatory variable of interest; state and year fixed effects; and time-varying covariates related to the costs of food, health care, housing, employment, other SNAP policies, and Temporary Assistance for Needy Families spending.ResultsHIV diagnoses within states had a statistically significant association with state income limits for SNAP eligibility (incidence rate ratio [IRR], 0.94 per increase in the income limit by 35% of federal poverty level; 95% CI, 0.91–0.98), but no association with state asset limits (increased asset limit vs no change: IRR, 1.02; 95% CI, 0.94–1.10; eliminated asset limit vs no change: IRR, 1.04; 95% CI, 0.99–1.10).ConclusionsState income limits for SNAP eligibility were inversely associated with the number of new HIV diagnoses for states between 2010 and 2014. Proposals to eliminate the use of broad-based categorical eligibility to increase the income limit for SNAP may undercut efforts to end the HIV epidemic in the United States.