bluuebunny/crossref_metadata_2025_split huggingface dataset:
The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration
Authors: Chen Jialin , Mo Qingyun , Sheng Renwang , Zhu Aijing , Ling Chen , Luo Yifan , Zhang Aini , Chen Zhixuan , Yao Qingqiang , Cai Zhuoying , Zhang Wei 
Published: December 2021

Abstract
Background
With the development of tissue engineering, enhanced tendon regeneration could be achieved by exploiting suitable cell types and biomaterials. The accessibility, robust cell amplification ability, superior tendon differentiation potential, and immunomodulatory effects of human periodontal ligament stem cells (hPDLSCs) indicate their potential as ideal seed cells for tendon tissue engineering. Nevertheless, there are currently no reports of using PDLSCs as seed cells. Previous studies have confirmed the potential of silk scaffold for tendon tissue engineering. However, the biomimetic silk scaffold with tendon extracellular matrix (ECM)-like structure has not been systematically studied for in situ tendon regeneration. Therefore, this study aims to evaluate the effects of hPDLSCs and biomimetic silk scaffold on in situ tendon regeneration.

Methods
Human PDLSCs were isolated from extracted wisdom teeth. The differentiation potential of hPDLSCs towards osteo-, chondro-, and adipo-lineage was examined by cultured in different inducing media. Aligned and random silk scaffolds were fabricated by the controlled directional freezing technique. Scaffolds were characterized including surface structure, water contact angle, swelling ratio, degradation speed and mechanical properties. The biocompatibility of silk scaffolds was evaluated by live/dead staining, SEM observation, cell proliferation determination and immunofluorescent staining of deposited collagen type I. Subsequently, hPDLSCs were seeded on the aligned silk scaffold and transplanted into the ruptured rat Achilles tendon. Scaffolds without cells served as control groups. After 4 weeks, histology evaluation was carried out and macrophage polarization was examined to check the repair effects and immunomodulatory effects.

Results
Human PDLSCs were successfully isolated, and their multi-differentiation potential was confirmed. Compared with random scaffold, aligned silk scaffold had more elongated and aligned pores and promoted the proliferation and ordered arrangement of hPDLSCs. After implantation into rat Achilles tendon defect, hPDLSCs seeded aligned silk scaffold enhanced tendon repair with more tendon-like tissue formation after 4 weeks, as compared to the scaffold-only groups. Higher expression of CD206 and lower expression of iNOS, IL-1β and TNF-α were found in the hPDLSCs seeded aligned silk scaffold group, which revealed its modulation effect of macrophage polarization from M1 to M2 phenotype.

Conclusions
In summary, this study demonstrates the efficacy of hPDLSCs as seed cells and aligned silk scaffold as a tendon-mimetic scaffold for enhanced tendon tissue engineering, which may have broad implications for future tendon tissue engineering and regenerative medicine researches.
Prognostic value of immune-related genes and comparative analysis of immune cell infiltration in lung adenocarcinoma: sex differences
Authors: Fan Tao , Li Chunxiang , He Jie 
Published: December 2021

Abstract
Background
Lung adenocarcinoma (LUAD) is one of the most important subtypes of lung cancer. Compared with male LUAD patients, female patients have a higher incidence, but better long-term survival rate, with unknown reasons. In this study, we aimed to explore the effect of sex differences on immune cell infiltration in lung tumor microenvironment (TME), and tried to clarify the reasons for the different clinical characteristics of male and female LUAD patients, by conducting a comparative analysis of the TME.

Methods
Using ESTIMATE algorithm, we calculated immune and stromal scores of tumor samples downloaded from TCGA database according to immune or stromal components in TME. GO and KEGG enrichment analysis were conducted to reveal biological processes of these intersecting genes of high- and low-score groups. Cox regression analysis and protein–protein interaction (PPI) network analysis were performed to screen immune-related prognostic genes in female (CCR2, LCP2, and PTPRC) and male (BTK and CCR2) patients. Kaplan–Meier survival analysis was used to evaluate prognostic value of these identified genes. Mann–Whitney test was used to compare various indicators of male patients and female patients. The main results were subsequently validated in 420 cases from GSE72094.

Results
304 and 368 intersecting genes were identified in female and male patients, respectively. The immune score ranged from −943.17 to 3229.35 among female patients and from −541.75 to 3441.78 among male patients. The stromal score ranged from −1790.23 to 2097.27 among female patients and from −1786.94 to 1722.70 among male patients. The immune and stromal scores of women were higher than those of men (p < 0.05). CCR2, LCP2 and PTPRC were identified as the most important immune-related prognostic genes in female LUAD patients. BTK and CCR2 were identified as the most important immune-related prognostic genes in male LUAD patients. Female patients had a higher proportion of memory B cells than that of male patients, while the percentage of T cells CD4 naïve and resting NK cells was lower in female patients (p < 0.05).

Conclusions
This study comprehensively compared the differences in tumor immune microenvironment between male and female LUAD patients, and identified prognosis-related genes for patients of different sexes.
Three bedside techniques to quantify dynamic pulmonary hyperinflation in mechanically ventilated patients with chronic obstructive pulmonary disease
Authors: Roesthuis L. H. , van der Hoeven J. G. , Guérin C. , Doorduin J. , Heunks L. M. A. 
Published: December 2021

Abstract
Background
Dynamic pulmonary hyperinflation may develop in patients with chronic obstructive pulmonary disease (COPD) due to dynamic airway collapse and/or increased airway resistance, increasing the risk of volutrauma and hemodynamic compromise. The reference standard to quantify dynamic pulmonary hyperinflation is the measurement of the volume at end-inspiration (Vei). As this is cumbersome, the aim of this study was to evaluate if methods that are easier to perform at the bedside can accurately reflect Vei.

Methods
Vei was assessed in COPD patients under controlled protective mechanical ventilation (7 ± mL/kg) on zero end-expiratory pressure, using three techniques in a fixed order: (1) reference standard (Veireference): passive exhalation to atmosphere from end-inspiration in a calibrated glass burette; (2) ventilator maneuver (Veimaneuver): measuring the expired volume during a passive exhalation of 45s using the ventilator flow sensor; (3) formula (Veiformula): (Vt × Pplateau)/(Pplateau − PEEPi), with Vt tidal volume, Pplateau is plateau pressure after an end-inspiratory occlusion, and PEEPi is intrinsic positive end-expiratory pressure after an end-expiratory occlusion. A convenience sample of 17 patients was recruited.

Results
Veireference was 1030 ± 380 mL and had no significant correlation with Pplateau (r2 = 0.06; P = 0.3710) or PEEPi (r2 = 0.11; P = 0.2156), and was inversely related with Pdrive (calculated as Pplateau −PEEPi) (r2 = 0.49; P = 0.0024). A low bias but rather wide limits of agreement and fairly good correlations were found when comparing Veimaneuver and Veiformula to Veireference. Vei remained stable during the study period (low bias 15 mL with high agreement (95% limits of agreement from − 100 to 130 mL) and high correlation (r2 = 0.98; P < 0.0001) between both measurements of Veireference).

Conclusions
In patients with COPD, airway pressures are not a valid representation of Vei. The three techniques to quantify Vei show low bias, but wide limits of agreement.
Greedy sensor selection based on QR factorization
Authors: Kim Yoon Hak 
Published: December 2021

AbstractWe address the problem of selecting a given number of sensor nodes in wireless sensor networks where noise-corrupted linear measurements are collected at the selected nodes to estimate the unknown parameter. Noting that this problem is combinatorial in nature and selection of sensor nodes from a large number of nodes would require unfeasible computational cost, we propose a greedy sensor selection method that seeks to choose one node at each iteration until the desired number of sensor nodes are selected. We first apply the QR factorization to make the mean squared error (MSE) of estimation a simplified metric which is iteratively minimized. We present a simple criterion which enables selection of the next sensor node minimizing the MSE at iterations. We discuss that a near-optimality of the proposed method is guaranteed by using the approximate supermodularity and also make a complexity analysis for the proposed algorithm in comparison with different greedy selection methods, showing a reasonable complexity of the proposed method. We finally run extensive experiments to investigate the estimation performance of the different selection methods in various situations and demonstrate that the proposed algorithm provides a good estimation accuracy with a competitive complexity when compared with the other novel greedy methods.
Mobility-aware personalized service recommendation in mobile edge computing
Authors: Zhang Hongxia , Dong Yanhui , Yang Yongjin 
Published: December 2021

AbstractWith the proliferation of smartphones and an increasing number of services provisioned by clouds, mobile edge computing (MEC) is emerging as a complementary technology of cloud computing. It could provide cloud resources and services by local mobile edge servers, which are normally nearby users. However, a significant challenge is aroused in MEC because of the mobility of users. User trajectory prediction technologies could be used to cope with this issue, which has already played important roles in service recommendation systems with MEC. Unfortunately, little attention and work have been given in service recommendation systems considering users mobility. Thus, in this paper, we propose a mobility-aware personalized service recommendation (MPSR) approach based on user trajectory and quality of service (QoS) predictions. In the proposed method, users trajectory is firstly discovered by a hybrid long-short memory network. Then, given users trajectories, service QoS is predicted, considering the similarity of different users and different edge servers. Finally, services are recommended by a center trajectory strategy through MPSR. Experimental results on a real dataset show that our proposed approach can outperform the traditional recommendation approaches in terms of accuracy in mobile edge computing.
Fractional N-Laplacian boundary value problems with jumping nonlinearities in the fractional Orlicz–Sobolev spaces
Authors: Choi Q-Heung , Jung Tacksun 
Published: December 2021

AbstractWe investigate the multiplicity of solutions for problems involving the fractional N-Laplacian. We obtain three theorems depending on the source terms in which the nonlinearities cross some eigenvalues. We obtain these results by direct computations with the eigenvalues and the corresponding eigenfunctions for the fractional N-Laplacian eigenvalue problem in the fractional Orlicz–Sobolev spaces, the contraction mapping principle on the fractional Orlicz–Sobolev spaces and Leray–Schauder degree theory.
On initial inverse problem for nonlinear couple heat with Kirchhoff type
Authors: Nam Danh Hua Quoc 
Published: December 2021

AbstractThe main objective of the paper is to study the final model for the Kirchhoff-type parabolic system. Such type problems have many applications in physical and biological phenomena. Under some smoothness of the final Cauchy data, we prove that the problem has a unique mild solution. The main tool is Banach’s fixed point theorem. We also consider the non-well-posed problem in the Hadamard sense. Finally, we apply truncation method to regularize our problem. The paper is motivated by the work of Tuan, Nam, and Nhat [Comput. Math. Appl. 77(1):15–33, 2019].
Oscillation criteria of third-order neutral differential equations with damping and distributed deviating arguments
Authors: Wang Yakun , Meng Fanwei , Gu Juan 
Published: December 2021

AbstractOur objective in this paper is to study the oscillatory and asymptotic behavior of the solutions of third-order neutral differential equations with damping and distributed deviating arguments. New oscillation criteria are established, which are based on a refinement generalized Riccati transformation. An important tool for this investigation is the integral averaging technique. Moreover, we provide an example to illustrate the main results.
Does anthropogenic introduction of guppy fish (Poecilia reticulata) impact faunal species diversity and abundance in natural aquatic habitats? A systematic review protocol
Authors: Sasanami Misaki , Hustedt John , Alexander Neal , Horstick Olaf , Bowman Leigh , Hii Jeffrey , Echaubard Pierre , Braack Leo , Overgaard Hans J. 
Published: December 2021

Abstract
Background
The guppy fish (Poecilia reticulata) is a tropical fish ancestrally linked to northern South America and the Caribbean. It is known to be very tolerant of and adaptable to new environments, and able to consume multiple food sources, including mosquito larvae. Consequently, guppies have been frequently introduced to non-native ecosystems to control mosquito populations, resulting in near-global distribution. Indeed, due to the increasing need for mosquito-borne disease control, guppy releases will likely continue, however there are concerns about potential adverse impacts on other species, biodiversity, and certain ecosystem functions. The most significant of these is local species extinction, and by extension, reduced biodiversity. Yet, the extent of these impacts has not been evaluated by scientific review. Accordingly, this study will examine and evaluate whether anthropogenic introduction of guppy fish (Poecilia reticulata) has impacts on faunal species diversity and abundance in natural aquatic habitats. The results of this review may have implications for environmental management and policy and inform ecosystem-based integrated vector management and public health policy.

Methods
Relevant scientific articles will be identified by searching electronic databases. Articles will be included if they report changes or differences, associated with guppy fish introduction, in at least one of these population parameters: (1) abundance of individuals in any species, (2) total abundance of individuals in all species present, (3) species richness, (4) species diversity, and (5) community evenness. Each article will be assessed by at least two independent reviewers against pre-defined inclusion/exclusion criteria. Snowballing reference lists of included articles will be conducted. At least two reviewers will critically appraise all included studies using the Collaboration for Environmental Evidence Critical Appraisal Tool (CEECAT) and any discrepancies will be resolved by discussion between the two or adjudication by a third author if agreement is not reached. Each study will also be subjected to data extraction against pre-defined qualitative and quantitative outcomes and results will be tabulated/presented in figures where appropriate. A meta-analysis will be carried out on outcome parameters with sufficient evidence.
An autopsy case of unexpected death due to Addison’s disease caused by adrenal tuberculosis
Authors: Zhao Nan , Gao Yutong , Ni Chunsheng , Zhang Danfang , Zhao Xiulan , Li Yanlei , Sun Baocun 
Published: December 2021

Abstract
Background
Adrenal tuberculosis is difficult to diagnose due to non-specific symptom. Unexpected death due to adrenal insufficiency after trauma surgery is rare.

Case presentation
A 45-year-old man, who was admitted to hospital because of trauma to the right hand, died unexpectedly on the 13th day after replantation of amputated fingers. He was diagnosed with brain edema and diluted hyponatremia. Autopsy and histopathologic examination revealed severe brain edema combined with cerebellar tonsillar hernia, extensive destruction of adrenal gland caused by bilateral adrenal tuberculosis and right lung invasive pulmonary tuberculosis.

Conclusions
Trauma and pulmonary tuberculosis complicated with adrenal tuberculosis induced the adrenal crisis, which eventually lead to severe cerebral edema and hernia, and finally death from respiratory and circulatory failure. This autopsy and histopathologic examination suggested a possible pathophysiologic mechanism of sudden death due to diluted hyponatremia after trauma surgery.
Experiences of eating disorders from the perspectives of patients, family members and health care professionals: a meta-review of qualitative evidence syntheses
Authors: Gustafsson Sanna Aila , Stenström Karin , Olofsson Hanna , Pettersson Agneta , Wilbe Ramsay Karin 
Published: December 2021

Abstract
Background
Eating disorders are serious conditions that cause major suffering for patients and their families. Better knowledge about perceptions of eating disorders and their treatment, and which factors that facilitate or hinder recovery, is desired in order to develop the clinical work. We aimed to explore and synthesise experiences of eating disorders from the perspectives of those suffering from an eating disorder, their family members and health care professionals through an overarching meta-review of systematic reviews in the field.

Methods
A systematic literature search was conducted in the databases PubMed, PsycInfo, Scopus, and CINAHL. Inclusion criteria were systematic reviews of qualitative research on experiences, perceptions, needs, or desires related to eating disorders from the perspective of patients, family members or health care professionals. Systematic reviews that fulfilled the inclusion criteria were assessed for relevance and methodological limitations by at least two researchers independently. The key findings were analysed and synthesised into themes.

Results
We identified 17 systematic reviews that met our inclusion criteria. Of these, 13 reviews reported on the patients’ perspective, five on the family members’ perspective, and three on the health care professionals’ perspective. The study population in the reviews was predominantly girls and young women with anorexia nervosa, whilst systematic reviews focusing on other eating disorders were scarce. The findings regarding each of the three perspectives resulted in themes that could be synthesised into three overarching themes: 1) being in control or being controlled, 2) balancing physical recovery and psychological needs, and 3) trusting relationships.

Conclusions
There were several similarities between the views of patients, family members and health care professionals, especially regarding the significance of building trustful therapeutic alliances that also included family members. However, the informants sometimes differed in their views, particularly on the use of the biomedical model, which was seen as helpful by health care professionals, while patients and family members felt that it failed to address their psychological distress. Acknowledging these differences is important for the understanding of anorexia nervosa and other eating disorders, and may help clinicians to broaden treatment approaches to meet the expectations of patients and family members.
Widening the reach of family-based interventions for Anorexia Nervosa: autism-adaptations for children and adolescents
Authors: Loomes Rachel , Bryant-Waugh Rachel 
Published: December 2021

Abstract
Family-based interventions are widely recommended as a first line treatment for children and young people with Anorexia Nervosa. There is clear evidence that model-adherent delivery of specific eating disorder focused family interventions has the potential to help adolescents with Anorexia Nervosa, who have typically engaged in extreme dietary restriction and lost a significant amount of weight over a relatively short period of time. Nevertheless, there remains a significant number of young people with restrictive eating disorders for whom family-based interventions for Anorexia Nervosa prove less effective, suggesting adaptations may be indicated for some. In this paper we provide a rationale and structure for considering a number of possible adaptations to the delivery of family-based therapy for anorexia nervosa specifically intended to enhance its relevance and potential effectiveness for children and adolescents on the autism spectrum; a subgroup known to represent a significant minority in eating disorder populations who have been identified as having relatively poor outcomes.

Plain English summary
Past research has shown that certain family-based treatments are effective for many children and adolescents who develop Anorexia Nervosa. At the same time this type of treatment approach in its current form does not work for everyone. Recent research has highlighted the overlap between anorexia and autism and the need for the development of adaptations to existing treatments to better meet the requirements of people on the autism spectrum who develop anorexia. With this in mind we propose a number of autism-related adaptations that could be made to family-based treatments for anorexia. We hope that these might be formally tested in the future to see if these adaptations improve outcomes for this group of individuals.
Machine learning modeling of genome-wide copy number alteration signatures reliably predicts IDH mutational status in adult diffuse glioma
Authors: Nuechterlein Nicholas , Shapiro Linda G. , Holland Eric C. , Cimino Patrick J. 
Published: December 2021

AbstractKnowledge of 1p/19q-codeletion andIDH1/2mutational status is necessary to interpret any investigational study of diffuse gliomas in the modern era. While DNA sequencing is the gold standard for determining IDH mutational status, genome-wide methylation arrays and gene expression profiling have been used for surrogate mutational determination. Previous studies by our group suggest that 1p/19q-codeletion and IDH mutational status can be predicted by genome-wide somatic copy number alteration (SCNA) data alone, however a rigorous model to accomplish this task has yet to be established. In this study, we used SCNA data from 786 adult diffuse gliomas in The Cancer Genome Atlas (TCGA) to develop a two-stage classification system that identifies 1p/19q-codeleted oligodendrogliomas and predicts the IDH mutational status of astrocytic tumors using a machine-learning model. Cross-validated results on TCGA SCNA data showed near perfect classification results. Furthermore, our astrocytic IDH mutation model validated well on four additional datasets (AUC = 0.97, AUC = 0.99, AUC = 0.95, AUC = 0.96) as did our 1p/19q-codeleted oligodendroglioma screen on the two datasets that contained oligodendrogliomas (MCC = 0.97, MCC = 0.97). We then retrained our system using data from these validation sets and applied our system to a cohort of REMBRANDT study subjects for whom SCNA data, but not IDH mutational status, is available. Overall, using genome-wide SCNAs, we successfully developed a system to robustly predict 1p/19q-codeletion and IDH mutational status in diffuse gliomas. This system can assign molecular subtype labels to tumor samples of retrospective diffuse glioma cohorts that lack 1p/19q-codeletion and IDH mutational status, such as the REMBRANDT study, recasting these datasets as validation cohorts for diffuse glioma research.