bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endometrial regenerative cells with galectin-9 high-expression attenuate experimental autoimmune hepatitis
Authors: Wang Hongda , Zhao Yiming , Ren Bingbing , Qin Yafei , Li Guangming , Kong Dejun , Qin Hong , Hao Jingpeng , Sun Daqing , Wang Hao 
Published: October 2021

Abstract
Background
Autoimmune hepatitis (AIH) is a T cell-mediated immune disease that activates abnormally against hepatic antigens. We have previously reported that endometrial regenerative cells (ERCs) were a novel source of adult stem cells, which exhibiting with powerful immunomodulatory effects. Galectin-9 (Gal-9) is expressed in ERCs and plays an important role in regulating T cell response. This study aims to explore the role of ERCs in attenuation of AIH and to determine the potential mechanism of Gal-9 in ERC-mediated immune regulation.

Methods
ERCs were obtained from menstrual blood of healthy female volunteers. In vitro, ERCs were transfected with lentivirus vectors carrying LGALS9 gene and encoding green fluoresce protein (GFP-Gal-9-LVs) at a MOI 50, Gal-9 expression in ERCs was detected by ELISA and Q-PCR. CD4+ T cells isolated from C57BL/6 mouse spleen were co-cultured with ERCs. The proliferation of CD4+ T cells was detected by CCK-8 kit and the level of Lck/zap-70/LAT protein was measured by western blot. Furthermore, AIH was induced by ConA in C57BL/6 mice which were randomly assigned to untreated, unmodified ERC-treated and Gal-9 high-expressing ERC-treated groups. Histopathological score, liver function, CD4+/CD8+ cell infiltration in liver tissues, the proportion of immune cells in the spleen and liver, and ERC tracking were performed accordingly to assess the progression degree of AIH.

Results
After transfecting with GFP-Gal-9-LVs, Gal-9 expression in ERCs was significantly increased. Additionally, Gal-9 high-expressing ERCs effectively inhibited CD4+ T cell proliferation and downregulated CD4+ T cell active related proteins p-Lck/p-ZAP70/p-LAT in vitro. Furthermore, treatment with Gal-9 high-expressing ERCs restored liver function, ameliorated liver pathological damage, inhibit CD4+ and CD8+ T cell proliferation and suppress Th1 and Th17 cell response in the hepatitis mice. In addition, Gal-9 high-expressing ERCs further markedly enhanced the level of IL-10 but reduced the levels of IFN-γ, TNF-α, and IL-4 in mouse sera and liver. Cell tracking also showed that ERCs could migrate to the damaged liver organs.

Conclusions
The results suggested that Gal-9 was an essential modulator, which was required by ERCs in regulating T cell response and attenuating ConA-induced experimental hepatitis. And also, it provides a novel idea for the clinical treatment of AIH.
The critical role of epigenetic mechanism in PM2.5-induced cardiovascular diseases
Authors: Sun Qinglin , Ren Xiaoke , Sun Zhiwei , Duan Junchao 
Published: October 2021

AbstractCardiovascular disease (CVD) has become the leading cause of death worldwide, which seriously threatens human life and health. Epidemiological studies have confirmed the occurrence and development of CVD are closely related to air pollution. In particular, fine particulate matter (PM2.5) is recognized as an important environmental factor contributing to increased morbidity, mortality and hospitalization rates among adults and children. However, the underlying mechanism by which PM2.5 promotes CVD development remains unclear. With the development of epigenetics, recent studies have shown that PM2.5 exposure may induce or aggravate CVD through epigenetic changes. In order to better understand the potential mechanisms, this paper reviews the epigenetic changes of CVD caused by PM2.5. We summarized the epigenetic mechanisms of PM2.5 causing cardiovascular pathological damage and functional changes, mainly involving DNA methylation, non-coding RNA, histone modification and chromosome remodeling. It will provide important clues for exploring the biological mechanisms affecting cardiovascular health.
Experiences with a violence and mental health safety protocol for a randomized controlled trial to support youth living with HIV
Authors: Merrill Katherine G. , Mwansa Jonathan K. , Miti Sam , Burke Virginia M. , Abrams Elizabeth A. , Frimpong Christiana , Decker Michele R. , Campbell Jacquelyn C. , Denison Julie A. 
Published: October 2021

Abstract
Background
Safety protocols are an essential component of studies addressing violence and mental health but are rarely described in the published literature from Sub-Saharan Africa. We designed and implemented a safety protocol within Project YES! (Youth Engaging for Success), which enrolled 276 youth living with HIV (ages 15–24 years) in a randomized controlled trial of a peer-mentoring intervention across four HIV clinics in Ndola, Zambia.

Methods
Youth who reported severe violence and/or suicidal thoughts on research surveys or during meetings with youth peer mentors (YPM) were referred to designated healthcare providers (HCP). We explored experiences with the safety protocol using: a) monitoring data of referrals, and b) in-depth interviews with youth (n = 82), HCP (n = 10), YPM (n = 8), and staff (n = 6). Descriptive statistics were generated and thematic analysis of coded transcripts and written memos performed.

Results
Nearly half of youth enrolled (48% of females, 41% of males) were referred to a HCP at least once. The first referral was most often for sexual violence (35%) and/or suicidal ideation/depression (29%). All referred youth aged 15–17 years and over 80% of referred youth aged 18 + agreed to see a HCP. HCP referred 15% for additional services outside the clinic. Twenty-nine youth, all HCP, all YPM, and all staff interviewed discussed the safety protocol. Most youth felt “encouraged,” “helped,” “unburdened,” and “relieved” by their meetings with HCP; some expressed concerns about meeting with HCP. The safety protocol helped HCP recognize the need to integrate care for violence and mental health with medication adherence support. HCP, YPM, and study staff raised implementation challenges, including youth choosing not to open up to HCP, time and resource constraints, deficiencies in HCP training, and stigma and cultural norms inhibiting referrals outside the clinic for emotional trauma and mental health.

Conclusions
Implementing a safety protocol within an HIV clinic-based research study is possible and beneficial for youth and HCP alike. Implementation challenges underscore that HCP in Zambia work in over-stretched healthcare systems. Innovative strategies must address deficiencies in training and resources within HIV clinics and gaps in coordination across services to meet the overwhelming need for violence and mental health services among youth living with HIV.
Preparation and assistant-dyeing of formaldehyde-free amphoteric acrylic retanning agent
Authors: Ma Jian zhong , Liu Qiwu , Wu Mengxin , Tian Zhenhua 
Published: October 2021

Abstract
With the enhancement of environmental protection consciousness, concerns have been raised about non-toxic and biodegradable leather retanning agents. According to the European standard 2002/231/EC, the free formaldehyde content of leather products should be less than 150 mg/kg. As one of the retanning agents in the market, the content of free formaldehyde in the Multifunctional retanning agent (MTA) is 372.22 mg/kg and higher than the limit value. In this work, glutaraldehyde as an alternative of formaldehyde was used to modify acrylic polymer and an amphoteric acrylic retanning agent was prepared. Then it was used in retanning process, and its retanning and assistant-dyeing properties were investigated. The results showed that the free formaldehyde content of amphoteric acrylic retanning agent modified with glutaraldehyde was only 4.17 mg/kg. Meanwhile, the presence of amino groups in the amphoteric acrylic retanning agent improved the dyeing properties of leather by electrostatic attraction. Compared with the leather treated with anionic acrylic retanning agent, the residual dye concentration of the dyeing effluent of the retanned leather with amphoteric acrylic retanning agent decreased from 17.4 mg/L to 10.0 mg/L, and the dyed leather had better resistances to friction and water-washing. In addition, the BOD5/COD value of the wastewater after Mannich base polymer retanning was only 0.32, indicating that the retanning agent was biodegradable. Moreover, the leather retanned with amphoteric acrylic retanning agent had good thermal stability, fullness and physical and mechanical properties.

Graphical abstract
Marine-derived pipeline anticancer natural products: a review of their pharmacotherapeutic potential and molecular mechanisms
Authors: Seyed Mohamed Ali , Ayesha Siddiqua 
Published: October 2021

AbstractBackgroundCancer is a complex and most widespread disease and its prevalence is increasing worldwide, more in countries that are witnessing urbanization and rapid industrialization changes. Although tremendous progress has been made, the interest in targeting cancer has grown rapidly every year. This review underscores the importance of preventive and therapeutic strategies.Main textNatural products (NPs) from various sources including plants have always played a crucial role in cancer treatment. In this growing list, numerous unique secondary metabolites from marine sources have added and gaining attention and became potential players in drug discovery and development for various biomedical applications. Many NPs found in nature that normally contain both pharmacological and biological activity employed in pharmaceutical industry predominantly in anticancer pharmaceuticals because of their enormous range of structure entities with unique functional groups that attract and inspire for the creation of several new drug leads through synthetic chemistry. Although terrestrial medicinal plants have been the focus for the development of NPs, however, in the last three decades, marine origins that include invertebrates, plants, algae, and bacteria have unearthed numerous novel pharmaceutical compounds, generally referred as marine NPs and are evolving continuously as discipline in the molecular targeted drug discovery with the inclusion of advanced screening tools which revolutionized and became the component of antitumor modern research.ConclusionsThis comprehensive review summarizes some important and interesting pipeline marine NPs such as Salinosporamide A, Dolastatin derivatives, Aplidine/plitidepsin (Aplidin®) and Coibamide A, their anticancer properties and describes their mechanisms of action (MoA) with their efficacy and clinical potential as they have attracted interest for potential use in the treatment of various types of cancers.
Comparison of arthropod communities between high and low input maize farms in Mexico
Authors: del-Val E. , Ramírez E. , Astier M. 
Published: October 2021

Abstract
Background
Animal communities are vulnerable to agricultural practices. Intensive farming considerably reduces overall arthropod diversity, but not necessarily pest abundance. Natural control of herbivores in agroecosystems is accomplished by predators and parasitoids, but in intensified agricultural regimes, the chemical control used to reduce pest abundances also affects pests’ natural enemies. To achieve more sustainable agriculture, there is a need to better understand the susceptibility of predators to conventional management.

Methods
In order to quantify the arthropod diversity associated with different schemes of agricultural management of maize, we evaluated agricultural fields under two contrasting management regimens in Michoacán, México during the spring–summer cycle of 2011. Arthropod communities were evaluated in plots with conventional high-input versus low-input agriculture in two sites—one rainfed and one with irrigation. The experimental units consisted of twelve 1 ha agricultural plots. To sample arthropods, we used 9 pitfall traps per agricultural plot.

Results
During the sampling period, we detected a total of 14,315 arthropods belonging to 12 Orders and 253 morphospecies. Arthropod community composition was significantly different between the sites, and in the rain-fed site, we also found differences between management practices. Predators, particularly ants, were more abundant in low-input sites. Herbivory levels were similar in all fields, with an average of 18% of leaf area lost per plant.

Conclusions
Our results suggest that conventional farming is not reducing herbivore abundances or damage inflicted to plants, but is affecting arthropod predators. We discuss repercussions for sustainable agriculture.
Crosscorrelation migration of microseismic source locations with hybrid imaging condition
Authors: Wu Shaojiang , Wang Yibo , Xie Fei , Chang Xu 
Published: October 2021

Locating microseismic sources is critical to monitoring the hydraulic fractures that occur during fluid extraction/injection in unconventional oil/gas exploration, geothermal operations, and CO2 sequestration. Waveform-based seismic location methods can reliably and automatically image weak microseismic source locations without phase picking. Among them, the crosscorrelation migration (CCM) method can avoid excitation time scanning by generating virtual gathers. We have adopted a CCM location method based on the hybrid imaging condition (HIC). There are four main steps in the implementation of this method: (1) selection of receivers with good azimuthal coverage, (2) generation of virtual gathers by correlating the reference receiver with the rest of the receivers, (3) summation of back projections in the virtual gathers, and (4) multiplication of all summations. The CCM-HIC method is tested on synthetic and field data sets, and the results are compared with those obtained by the conventional summation imaging condition and multiplication imaging condition. The comparison results determine that CCM-HIC is sufficiently robust to obtain a source image with better stability and higher spatial resolution, despite the presence of strong noise.
Anderson-accelerated augmented Lagrangian for extended waveform inversion
Authors: Aghazade Kamal , Gholami Ali , Aghamiry Hossein S. , Operto Stéphane 
Published: October 2021

The augmented Lagrangian (AL) method provides a flexible and efficient framework for solving extended-space full-waveform inversion (FWI), a constrained nonlinear optimization problem whereby we seek model parameters and wavefields that minimize the data residuals and satisfy the wave-equation constraint. The AL-based wavefield reconstruction inversion, also known as iteratively refined wavefield reconstruction inversion, extends the search space of FWI in the source dimension and decreases the sensitivity of the inversion to the initial model accuracy. Furthermore, it benefits from the advantages of the alternating direction method of multipliers, such as generality and decomposability for dealing with nondifferentiable regularizers, e.g., total variation regularization, and large-scale problems, respectively. In practice, any extension of the method aiming at improving its convergence and decreasing the number of wave-equation solves would have great importance. To achieve this goal, we recast the method as a general fixed-point iteration problem, which enables us to apply sophisticated acceleration strategies such as Anderson acceleration. The accelerated algorithm stores a predefined number of previous iterates and uses their linear combination together with the current iteration to predict the next iteration. We investigate the performance of our accelerated algorithm on a simple checkerboard model and the benchmark Marmousi II and 2004 BP salt models through numerical examples. These numerical results confirm the effectiveness of our algorithm in terms of convergence rate and the quality of the final estimated model.
Physiological responses of freshwater insects to salinity: molecular-, cellular- and organ-level studies
Authors: Silver Sydney , Donini Andrew 
Published: October 2021

ABSTRACTSalinization of freshwater is occurring throughout the world, affecting freshwater biota that inhabit rivers, streams, ponds, marshes and lakes. There are many freshwater insects, and these animals are important for ecosystem health. These insects have evolved physiological mechanisms to maintain their internal salt and water balance based on a freshwater environment that has comparatively little salt. In these habitats, insects must counter the loss of salts and dilution of their internal body fluids by sequestering salts and excreting water. Most of these insects can tolerate salinization of their habitats to a certain level; however, when exposed to salinization they often exhibit markers of stress and impaired development. An understanding of the physiological mechanisms for controlling salt and water balance in freshwater insects, and how these are affected by salinization, is needed to predict the consequences of salinization for freshwater ecosystems. Recent research in this area has addressed the whole-organism response, but the purpose of this Review is to summarize the effects of salinization on the osmoregulatory physiology of freshwater insects at the molecular to organ level. Research of this type is limited, and pursuing such lines of inquiry will improve our understanding of the effects of salinization on freshwater insects and the ecosystems they inhabit.
Recognizing and mediating bureaucratic barriers: increasing access to care through small and medium-sized private providers in Kenya
Authors: Suchman Lauren , Owino Edward , Montagu Dominic 
Published: October 2021

Background: Equitable access to health services can be constrained in countries where private practitioners make up a large portion of primary care providers. Expanding purchasing arrangements has helped many countries integrate private providers into government-supported payment schemes, reducing financial barriers to care. However, private providers often must go through an onerous accreditation process to enroll in these schemes. The difficulties of this process are exacerbated where health policy is changed often and low-level bureaucrats must navigate these shifts at their own discretion. This paper analyzes one initiative to increase private provider accreditation with social health insurance (SHI) in Kenya by creating an intermediary between providers and “street-level” SHI bureaucrats. Methods: This paper draws on 126 semi-structured interviews about SHI accreditation experience with private providers who were members of a franchise network in Kenya. It also draws on four focus group discussions conducted with franchise representatives who provided accreditation support to the providers and served as liaisons between the franchised providers and local SHI offices. There was a total of 20 participants across all four focus groups. Results: In a governance environment where regulations are weak and impermanent, street-level bureaucrats often created an accreditation process that was inconsistent and opaque. Support from the implementing organizations increased communication between SHI officials and providers, which clarified rules and increased providers’ confidence in the system. The intermediaries also reduced bureaucrats’ ability to apply regulations at will and helped to standardize the accreditation process for both providers and bureaucrats. Conclusions: We conclude that intermediary organizations can mitigate institutional weaknesses and facilitate process efficiency. However, intermediaries only have a temporary role to play where there is potential to: 1) directly increase private providers’ power in a complex regulatory system; 2) reform the system itself to be more responsive to the limitations of on-the-ground implementation.
A spatio-temporal atlas of the developing fetal brain with spina bifida aperta
Authors: Fidon Lucas , Viola Elizabeth , Mufti Nada , David Anna L. , Melbourne Andrew , Demaerel Philippe , Ourselin Sébastien , Vercauteren Tom , Deprest Jan , Aertsen Michael 
Published: October 2021

Background: Spina bifida aperta (SBA) is a birth defect associated with severe anatomical changes in the developing fetal brain. Brain magnetic resonance imaging (MRI) atlases are popular tools for studying neuropathology in the brain anatomy, but previous fetal brain MRI atlases have focused on the normal fetal brain. We aimed to develop a spatio-temporal fetal brain MRI atlas for SBA. Methods: We developed a semi-automatic computational method to compute the first spatio-temporal fetal brain MRI atlas for SBA. We used 90 MRIs of fetuses with SBA with gestational ages ranging from 21 to 35 weeks. Isotropic and motion-free 3D reconstructed MRIs were obtained for all the examinations. We propose a protocol for the annotation of anatomical landmarks in brain 3D MRI of fetuses with SBA with the aim of making spatial alignment of abnormal fetal brain MRIs more robust. In addition, we propose a weighted generalized Procrustes method based on the anatomical landmarks for the initialization of the atlas. The proposed weighted generalized Procrustes can handle temporal regularization and missing annotations. After initialization, the atlas is refined iteratively using non-linear image registration based on the image intensity and the anatomical land-marks. A semi-automatic method is used to obtain a parcellation of our fetal brain atlas into eight tissue types: white matter, ventricular system, cerebellum, extra-axial cerebrospinal fluid, cortical gray matter, deep gray matter, brainstem, and corpus callosum. Results: An intra-rater variability analysis suggests that the seven anatomical land-marks are sufficiently reliable. We find that the proposed atlas outperforms a normal fetal brain atlas for the automatic segmentation of brain 3D MRI of fetuses with SBA. Conclusions: We make publicly available a spatio-temporal fetal brain MRI atlas for SBA, available here: https://doi.org/10.7303/syn25887675. This atlas can support future research on automatic segmentation methods for brain 3D MRI of fetuses with SBA.
Inclusive Growth in Northern and Southern Central Java
Authors: Wuranti Hayu 
Published: October 2021

Inclusive growth is one of the visions of sustainable development. Economic growth of a region is said to be inclusive if it is able to reduce poverty, reduce inequality and absorb more labour. Central Java is one of the regions with fairly high economic growth but has various social problems. Therefore, this study wants to see the inclusiveness of economic growth in the North and South Central Java region. The research is based on data from 35 districts in Central Java, which adopts the concept formulated by Klasen (2010) regarding the Poverty-Equivalent Growth Rate (PEGR). The research shows that during the 2018-2020 period, Central Java had not consistently has inclusive growth. Meanwhile, inclusive growth for the three indicators in 2018 mostly occurred in the northern part of Central Java but was more and more balanced.