bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Resolution Detection of Quantitative Trait Loci for Seven Important Yield Components in Wheat (Triticum aestivum L.) using a High-Density SALF-Seq Genetic Map
Authors: Li Tao , Li Qiao , Wang Jinhui , Yang Zhao , Tang Yanyan , Su Yan , Zhang Juanyu , Qiu Xvebing , Pu Xi , Pan Zhifen , Zhang Haili , Liang Junjun , Liu Zehou , Li Jun , Yang Wuyun , Yu Maoqun , Long Hai , Wei Yuming , Deng Guangbing 
Published: August 2021

Abstract
Background: Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with wheat (Triticum aestivum L.) grain yield. To detect quantitative trait loci (QTL) associated with them, 193 recombinant inbred lines derived from two elite winter wheat varieties Chuanmai42 and Chuanmai39 were employed to perform QTL mapping in six or eight environments. Results: A total of 30 QTLs on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 4A, 5A, 5B, 6A, 6D, 7A, 7B and 7D were identified. Among them, six major QTLs QTgw.cib-6A.1, QTgw.cib-6A.2, QGw.cib-6A, QGl.cib-3A, QGl.cib-6A, and QSl.cib-2D explaining 5.96-23.75% of the phenotypic variance were detected in multiple environments and showed strong and stable effects on corresponding traits. Three QTL clusters on chromosomes 2D and 6A containing 10 QTLs were also detected, which showed significant pleiotropic effects on multiple traits. Additionally, three Kompetitive Allele Specific PCR (KASP) markers linked to five of these major QTLs were developed. Candidate genes of QTgw.cib-6A.1/QGl.cib-6A and QGl.cib-3A were analyzed based on the spatiotemporal expression patterns, gene annotation, and orthologous search. Conclusions: Six major QTLs for TGW, GL, GW and SL were detected. Three KASP markers linked with five of these major QTLs were developed. These QTLs and KASP markers will be useful for elucidating the genetic architecture of grian yield and developing new wheat varieties with high and stable yield in wheat.
Upper airway gene expression reveals a more robust innate and adaptive immune response to SARS-CoV-2 in children compared with older adults
Authors: Mick Eran , Tsitsiklis Alexandra , Spottiswoode Natasha , Caldera Saharai , Serpa Paula , Detweiler Angela , Neff Norma , Pisco Angela Oliveira , Li Lucy , Retallack Hanna , Ratnasiri Kalani , Williamson Kayla , Soesanto Victoria , Simões Eric , Kistler A. , Wagner Brandie , DeRisi Joseph , Ambroggio Lilliam , Mourani Peter , Langelier Charles 
Published: August 2021

Abstract
Unlike other respiratory viruses, SARS-CoV-2 disproportionately causes severe disease in older adults and only rarely in children. To investigate whether differences in the upper airway immune response could contribute to this disparity, we compared nasopharyngeal gene expression in 83 children (<19-years-old; 38 with SARS-CoV-2, 11 with other respiratory viruses, 34 with no virus) and 154 adults (>40-years-old; 45 with SARS-CoV-2, 28 with other respiratory viruses, 81 with no virus). Expression of interferon-stimulated genes (ISGs) was robustly activated in both children and adults with SARS-CoV-2 compared to the respective non-viral groups, with only relatively subtle distinctions. Children, however, demonstrated markedly greater upregulation of pathways related to B cell and T cell activation and proinflammatory cytokine signaling, including TNF, IFNγ, IL-2 and IL-4 production. Cell type deconvolution confirmed greater recruitment of B cells, and to a lesser degree macrophages, to the upper airway of children. Only children exhibited a decrease in proportions of ciliated cells, the primary target of SARS-CoV-2, upon infection with the virus. These findings demonstrate that children elicit a more robust innate and adaptive immune response to SARS-CoV-2 infection in the upper airway that likely contributes to their protection from severe disease in the lower airway.
Unexpected Favorable Outcome To Sintinib Monotherapy in A Relapse PDAC Patient With High TMB
Authors: zhang Fan , Li Xiaomin , liu haisheng , liu Rongfeng , zhou zhiguo , zhang Yi , chen Jingli , tian Ye , pan chaohu , Meng Qingju , Liu Yibing 
Published: August 2021

Abstract
Background Immune checkpoint inhibitors have not been widely used for pancreatic cancer treatment probably because of low immunogenic or stromal fibrotic tumor, among which only pembrolizumab was recommended for DNA mismatch repair deficiency (dMMR) by NCCN. Case presentation Here we report a 56-year-old man suffered from pancreatic ductal cancer (PDAC) with liver metastasis after radical surgery. NGS revealed remarkably high burden of TMB (49.92Muts/Mb) and microsatellite stability (MSS). Sintilimab monotherapy was continuously administrated after failure of combined chemotherapy, leading to stable disease within 22 months and few immunotherapy related AEs happened. Conclusions To our best knowledge, this is the first to report a good outcome achieving 22 months progression-free survival after PDAC metastasis with monotherapy of an immune checkpoint inhibitor. TMB may serve as a potential immunotherapy-related predictor in PDAC patients and help physicians make optimum clinical strategy.
Non-Traumatic Tile Type B3 Pelvic Fracture Combined With Garden Type III Femoral Neck Fracture Caused By Hypocalcaemia-Induced Convulsions
Authors: Dou Minghan , Ren Guangkai , Yuan Baoming , Peng Chuangang , Wu Dankai 
Published: August 2021

Abstract
BACKGROUND Non-traumatic fractures caused by convulsions are relatively rare and are often overlooked due to the lack of obvious evidence of injury. Non-traumatic fractures due to convulsions are diverse, but the concomitant of pelvic and femoral fractures has not been reported previously. CASE SUMMARY A 47-year-old woman with a no significant medical history, was taken to the nearest hospital after one episode of generalized tonic-clonic seizure that had occurred during sleep. After the postictal phase, the patient regained consciousness and experienced diffuse pain in the inguinal regions bilaterally with inability to stand or walk. The entire attack was witnessed by the family members who confirmed that there was not external trauma. Blood investigations revealed high creatinine and uric acid levels, along with a low calcium level. Conventional radiograph of the pelvis was performed to detect the cause of pain. The patient was noted to have a pelvic fracture and unilateral fracture of the neck of the femur. She was then transferred to our hospital for further management. The patient was diagnosed as having Tile type B3 pelvic fractures combined with a Garden type III femoral neck fracture on three-dimensional computed tomography (3D-CT). In view of her blood results, nephrology consultation was sought. The patient was identified as having a stage 5 chronic kidney disease (CKD), hypocalcemia, and hyperphosphatemia. However, neurological examination showed no abnormalities. No surgical intervention was adopted for her pelvic fractures. In addition to complete bed rest, closed reduction and percutaneous cannulated screw fixation was performed for the treatment of the right femoral neck fracture. An arteriovenous fistula was created for maintenance hemodialysis on the tenth postoperative day. At follow-up, twelve months follow-up after the operation, the patient had a normally gait and was satisfied with the outcome. CONCLUSION Patients who present with convulsive seizures especially patients with ion metabolism disorder should be examined thoroughly to ensure that no injury is missed.
Prediction of the rate of hand injuries in Singapore
Authors: Liau Glen Zi Qiang , Wong Joel Yat Seng , Tay Wei Xuan , Weng Jia Yi , Cheah Andre Eu Jin 
Published: August 2021

Abstract
Purpose

This study aims to analyze the correlation between the incidence rate of hand injuries and various construction and manufacturing indicators in Singapore. We hypothesize that the number of hand injuries is correlated to activity in the construction and manufacturing industries in Singapore.
Methods

20,764 patients who underwent hand surgeries in a tertiary institution between 2012 to 2018 were reviewed. Two independent, blinded observers extracted the frequency of hand surgeries performed from Electronic Medical Records. Economic indicators pertinent to Singapore’s labor force, construction and manufacturing industries were collected and smoothed by simple moving average of the prior 3 months. Results were analyzed using IBM SPSS v25.0.
Results

Significant independent univariate variables were Purchasing Manager Index for Manufacturing and Industrial Production Index. Multivariate regression of quarterly reported figures showed that Purchasing Manager Index for Manufacturing, Construction Index, Labor Productivity Change for Gross Domestic Product and Labor Productivity Change for Manufacturing were significantly correlated with hand injuries, with r square = 60.9%.
Conclusion

Quarterly economic indicators from construction and manufacturing industries can be used to predict the incidence of hand injuries with a 60.9% correlation. These findings may be useful for anticipating healthcare resource allocation to treat hand injuries.
Type of Study and Level of Evidence
Economic and decision, Level II.
Severe Radiological Manifestation in Patients with Diabetes and Pulmonary Tuberculosis: A Cross-sectional Study
Authors: Zhang Peize , Xiong Juan , Zhan Shenlin , Ren Tantan , Wang Yuxiang , Fu Liang , Deng Guofang 
Published: August 2021

Abstract
Purpose: Diabetes mellitus (DM) is believed to affect tuberculosis (TB) at multiple levels in disease control and treatment efficacy, but clinical and radiological presentation resulting from interaction of the two diseases is not known. We conducted a retrospective study to investigate how glycemic control impacts radiological and clinical manifestations in TB patients. Methods: A cross-sectional study was conducted on data obtained from medical records of 438 patients confirmed with TB-DM comorbidity at The Third people’s hospital of Shenzhen from April 1, 2014 to April 30, 2019. Their CT images were reviewed, and patients were divided into subgroups according to lung cavitation: with and without cavities, and number of segments showing pulmonary infiltration: <4 segment, 4-8 segment, >8 segment infiltrates. We then compared clinical parameters between these groups. Results: The median age of the patients was 50.0 years (IQR 43.3-56) and 86% (n=375) of them were male. Pulmonary cavities were found in 80.8% patients. 42.7% and 27.2% patients were seen to have infiltration involving 4-8 and >8 lung segments respectively. Patients presented with cavitation and infiltration involving a greater number of lung segments had significantly higher values of WBC, MONO%, GRA%, CRP, lower LYN% level and higher bacterial burden in sputum (P<0.001). Higher HbA1c and FBG were only observed in patients with lung cavities (P<0.001). There was no difference in positive ELISPOT.TB and PCT level between the groups regardless of presence or absence of lung cavity (P>0.9 and P=0.1 respectively). Lower HGB, ALB and higher PCT were observed in patients with infiltration involving more lung segments.Conclusion: Hyper-inflammation in peripheral blood was significantly associated with cavity and the number of lung lesions. Hyperglycemia was significantly associated with the development of lung cavity. Glycemic control and inflammation influenced radiographic manifestations in patients with TB-DM.
Complex Dynamics in an Eco-Epidemiological Model with the Cost of Anti-Predator Behaviors
Authors: Tan Yiping , Cai Yongli , Yao Ruoxia , Hu Maolin , Wang Weiming 
Published: August 2021

Abstract
In this paper, we investigate the complex dynamics of a predator-prey model with disease in the prey, which is characterized by the reduction of prey growth rate due to the anti-predator behavior. The value of this study lies in two aspects: Mathematically, it provides the existence and the stability of the equilibria and gives the existence of Hopf bifurcation. And epidemiologically, we find that the influence of the fear factor is complex: (i) the level of the population density decreases with the increasing of the fear factor; (ii) the cost of fear can destabilize the stability and benefit the emergency of the periodic behavior; (iii) the high level of fear can induce the extinction of the predator. These results may enrich the dynamics of the eco-epidemiological systems.
Stalled at the Intersection: Insurance Status and Disparities in Post Mastectomy Breast Reconstruction
Authors: Friedman-Eldar Orli , Burke Jonathan , Silva Iago de Castro , Baumrucker Camille C , Valle Fernando , Lessard Anne-Sophie , Kassira Wrood , Franceschi Dido , Kesmodel Susan B , Avisar Eli , Goel Neha , Möller Mecker G 
Published: August 2021

Abstract
PurposePost-mastectomy breast reconstruction (PMBR) is an important component of breast cancer treatment, but disparities relative to insurance status persist despite legislation targeting the issue. We aimed to study this relationship in a large health system combining a safety net hospital and a private academic center.MethodsData were collected on all patients who underwent mastectomy for breast cancer from 2011-2019 in a private academic center and an adjacent public safety-net hospital served by same surgical teams. Multivariable logistic regression was used to assess the effect of insurance status on PMBR, controlling for covariates that included socioeconomic, demographic, and clinical factors.ResultsOf 1,554 patients undergoing mastectomy for breast cancer, 753 (48.5%) underwent PMBR. Out of them, 741 had insurance type recorded, with 592 (79.9%) privately insured patients, 50 (6.7%) Medicare, 68 (9.2%) Medicaid, and 31 (4.2%) uninsured patients. Multivariable logistic regression showed a significantly lower likelihood of undergoing PMBR for uninsured (OR 6.9, 95% CI: 4.1-11.7; p<0.0001), Medicare (OR 2.0, (5% CI: 1.2-3.3; p=0.004), and Medicaid (OR 1.7, 95% CI:1.1-2.7; p=0.02) patients, compared with privately insured patients. Age, stage, race, and hospital type confounded this relationship.ConclusionPatients without health insurance have dramatically reduced access to PMBR compared to those with private insurance. Expanding access to this important procedure is essential to achieve greater health equity for breast cancer patients.
Control of Difference of Seismic Response Across Different Spans of High-Speed Railway Multi-Spans Simply Supported Bridge
Authors: Zhang Yuntai , Jiang Lizhong , Zhou Wangbao , Lai Zhipeng , Liu Xiang , Peng Kang , Wang Zheng , Feng Yulin 
Published: August 2021

Abstract
In this paper, the Difference of Seismic Response across Different Spans (DSR) in the longitudinal distribution of High-Speed Railway Multi-spans Simply Supported Bridge (HSRSB) under longitudinal earthquake excitation is investigated, and an evaluation method which can intuitively reflect the difference of seismic response is proposed. A feasible way to strengthen the connection stiffness between adjacent girders is proposed to control DSR. The rationality of the finite element model used is verified by comparing the numerical results with the experimental ones, showing a satisfactory agreement. Comparing the seismic response of a bridge model considering the subgrade-track constraints (BCTM) and a bridge model without subgrade-track constraints (BWTM), it is found that the DSR in the longitudinal distribution causes some new disadvantages, which are neglected in BWTM. The BCTM considering DLC generates a model called BCDM. The effect of the number of span on DSR are studied based on BCDM. The analysis of this model showed that DLC suppresses the DSR and reduces the seismic response of most bridge components. It also transfers the seismic disadvantage from the bridge part to the subgrade-track structure. As it is more convenient and cost-effective to repair the base plate of the subgrade than the bridge components after earthquake seismic event, this disadvantage transfer is in favor of forming a new anti-seismic system that subgrade-track structure is used to protect the bridge part.
Genomic analyses of 10,376 individuals in the Westlake BioBank for Chinese (WBBC) pilot project
Authors: Cong Peikuan , Bai Weiyang , Li Jinchen , Yang Mengyuan , Khederzadeh Saber , Gai Sirui , Li Nan , Liu Yuheng , Yu Shihui , Zhao Weiwei , Liu Junquan , Sun Yi , Zhu Xiaowei , Zhao Pianpian , Xia Jiangwei , Guan Penglin , Qian Yu , Tao Jianguo , Xu Lin , Tian Geng , wang Pingyu , Xie Shu-Yang , Qiu Mochang , Liu Keqi , Tang Beisha , Zheng Hou-Feng 
Published: August 2021

Abstract
Imbalance of genetic resources in global population restricts the understanding of complex traits across populations. Here, we initiated the Westlake BioBank for Chinese (WBBC) pilot project with 4,535 whole-genome sequencing (NGS) individuals and 5,841 high-density genotyping individuals. We identified 81.5 million SNPs and INDELs, of which 38.5% are novel. We found that 5.05% of the rare variants in WBBC were common in European population, and some trait-associated common variants in European had much lower allele frequency in Chinese. We provided a population-specific reference panel and an online imputation server (https://wbbc.westlake.edu.cn/) which could yield substantial improvement of imputation performance in Chinese population, especially for low-frequency and rare variants. By analyzing the singleton density of the WGS data, we found novel selection signatures in SNX29, DNAH1 and WDR1 genes, and the selection of the alcohol metabolism genes (ADH1A and ADH1B) strengthened from about 4,000 years ago in East Asia. Genetic evidence supported the corresponding geographical boundaries of the Qinling-Huaihe Line and Nanling Mountains, which separated the Han Chinese into subgroups, and we revealed that North Han was more homogeneous than South Han, and the history of effective population size of Lingnan began to deviate from the other three regions from 6,000 years ago. Significant selection of genes on epidermal cell differentiation was only observed in southern Chinese. Genetic divergence from north to south was more noticeable in the ancient than modern populations.
HPV and （ Neutrophil-to-lymphocyte ratio ） NLR are Prognostic Indicators of Laryngeal Cancer Patients Receiving Concurrent Chemoradiotherapy
Authors: Li Ke-jie , Lv Qi-yuan , Xu Yu-yan , Shao Zhen-yong , Zou Chang-Lin , Xie Rao-ying 
Published: August 2021

Abstract
Background and objectives: After receiving radical concurrent radiotherapy and chemotherapy, some patients with laryngeal cancer still have recurrence, and the recurrence time of different patients is different, which will pose a threat to the quality of life and survival of patients. The purpose of our study is to find out the factors that can predict the recurrence of laryngeal cancer, provide more accurate and individualized treatment for patients with laryngeal cancer, and improve the quality of life of patients.Methods：We collected follow-up data from 123 patients with laryngeal cancer who were admitted to the First Affiliated Hospital of Wenzhou Medical University between 2010 and 2015. These patients received only radical concurrent chemoradiotherapy after diagnosis. Univariate Cox analysis was used to find the factors related to Progression-free survival（PFS）in patients with laryngeal cancer. Multivariate Cox regression analysis was used to determine the factors and indicators that could predict patients' PFS. The Receiver Operating Characteristic curve（ROC curve） was used to determine the optimal truncation value of the forecast index.Results：Univariate Cox regression analysis showed that HPV, lymph node metastasis, NLR, （lymphocyte-to-monocyte Ratio）LMR and white blood cell count were related to PFS in patients with laryngeal cancer. Multivariate Cox regression analysis showed that positive HPV and NLR were effective predictors of PFS in laryngeal cancer. According to the ROC curve, the area under the curve of NLR is 0.743, and the optimal truncation value is 2.26.Conclusions：HPV and NLR are reliable predictors of PFS in patients with laryngeal cancer receiving concurrent chemoradiotherapy, which can provide help and suggestions for follow-up and individualized treatment of laryngeal cancer after treatment.
A Novel TMTP1-modified Theranostic Nanoplatform for Targeted in Vivo NIR-II Fluorescence Imaging-guided Chemotherapy of Cervical Cancer
Authors: Nuernisha Alifu , Ma Rong , Zhu Lijun , Du Zhong , Chen Shuang , Yan Ting , Alimu Gulinigaer , Zhang Linxue , Ma Cailing , Zhang Xueliang 
Published: August 2021

Abstract
BackgroundNear-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity and contrast, has attracted great attentions in biomedical research fields. However, most nanoprobes used for NIR-II fluorescence imaging have poor tumor-targeting ability and therapeutic efficiency. To overcome these limitations, a novel NIR-II-emissive theranostic nanoplatform for imaging and treatment of cervical cancer was designed and prepared. The NIR-II-emissive dye IR-783 and chemotherapy drug doxorubicin (DOX) were encapsulated into liposomes, and the tumor-targeting peptide TMTP1 was conjugated to the surface of the liposomes to form IR-783-DOX-TMTP1 nanoparticles (NPs) via self-assembly methods.ResultsThe IR-783-DOX-TMTP1 NPs showed strong NIR-II emission, excellent biocompatibility, a long lifetime, and low toxicity. Further, high-definition NIR-II fluorescence microscopy images of ear blood vessels and intratumor blood vessels were obtained from IR-783-DOX-TMTP1 NPs-stained mice with high spatial resolution under 808 nm laser excitation. Moreover, IR-783-DOX-TMTP1 NPs showed strong tumor targeting ability and high efficiently chemotherapeutic character towards cervical tumors. ConclusionsThe novel targeting and NIR-II-emissive IR-783-DOX-TMTP1 NPs have potential in diagnosis and therapy for cervical cancer.
Structural Insight into PRMT5 Inhibitors through Amalgamating Pharmacophore-Based Virtual Screening, ADME-Toxicity and Binding Energy Studies and Identify New Inhibitors by Molecular Docking.
Authors: Bathula Revanth , Sivan Sree kanth , Somadi Gururaj , Muddagoni Narasimha , Lanka Goverdhan , Potlapally Sarita Rajender 
Published: August 2021

Abstract
Protein arginine methyltransferase 5 (PRMT5) is a member of the methyltransferases family, a type II arginine enzyme that is crucial for many cellular processes and is associated with many cancer diseases. In this study, pharmacophore-based 3D QSAR modeling, virtual screening and binding free energy studies were carried out from a set of 61 potent compounds reported being inhibitors of PRMT5 protein. A five-point pharmacophore model (AADHR) was generated and this model is used to generate an atom-based 3-Dimensional quantitative structure-activity relationship (3D-QSAR). The obtained 3D-QSAR model has high correlation coefficient (R2 = 0.91), cross-validation coefficient (Q2 = 0.82), F value (140.3), low RMSE (0.47) and pearson R-value (0.91). A library of 329825 molecules (ChEMBL database) is screened with pharmacophore model to retrieve hit molecules that are further subjected for molecular docking to identify best fit-active conformations binding at the receptor site of PRMT5 protein. Further, we are calculated ADME and toxicity properties using QikProp module and pkCSM server and finally prioritized the lead molecules by binding free energy prediction.