bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety, immunogenicity, and protective efficacy of the intranasal live pertussis vaccine GamLPV in an infant monkey model (<i>Papio hamadryas</i>)
Authors: Kubrava D. T. , Medkova A. Yu. , Matua A. Z. , Kondzariya I. G. , Amichba A. A. , Trapsh Kh. Z. , Gamgiya L. V. , Kulikov S. G. , Sinyashina L. N. , Mikvabiya Z. Ya. , Karataev G. I. 
Published: December 2024

INTRODUCTION. The current progressive increase in pertussis incidence and infant mortality rates is due to the insufficient effectiveness of existing vaccines, both in Russia and worldwide. Previous clinical trials showed that healthy adult volunteers developed long-term antibacterial immunity after vaccination with GamLPV, an intranasal recombinant live pertussis vaccine developed by the National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya. Further clinical development of GamLPV in paediatric volunteers, including infants, requires preclinical studies in a newborn monkey model.AIM. This study aimed to evaluate the safety, immunogenicity, and protective efficacy of the GamLPV vaccine in infant hamadryas baboons (Papio hamadryas) challenged with pertussis after intranasal vaccination.MATERIALS AND METHODS. The study used 20 hamadryas baboons, including 7 infants aged 1–1.5 months, 7 mothers of these infants, and 6 control animals. The study examined the time course of changes in serum levels of specific IgG antibodies to pertussis toxin (PT) and filamentous haemagglutinin (FHA) by enzyme-linked immunosorbent assay (ELISA), monitored changes in serum levels of Bordetella pertussis antibodies by agglutination immunoassay, and detected B. pertussis DNA in oropharyngeal aspirates by real-time polymerase chain reaction.RESULTS. Intranasal GamLPV administration to infant baboons induced the production of specific IgG antibodies to PT and FHA (ELISA) and an increase in the total pertussis antibody titre (agglutination immunoassay). GamLPV did not cause any injection site or systemic reactions. There were no changes in complete blood counts and serum biochemistry profiles after vaccination. The protective efficacy of GamLPV against B. pertussis was demonstrated in challenge tests, where immunised animals had no clinical signs or laboratory findings indicative of pertussis in contrast to controls.CONCLUSIONS. The study demonstrated the safety and immunogenicity of the intranasal live pertussis vaccine GamLPV in newborn hamadryas baboons. GamLPV shows promise in the primary vaccination of infants, the revaccination of children and adults, and the development of herd immunity against pertussis in families.
Preclinical and clinical development of preventive rotavirus vaccines: special considerations
Authors: Korovkin A. S. , Gorenkov D. V. , Soldatov A. A. , Merkulov V. A. 
Published: December 2024

INTRODUCTION. Vaccination is recognised as the only effective method for preventing rotavirus disease. Rotavirus remains a leading cause of death in young children, mainly, in developing countries. Currently, oral rotavirus vaccines for infant immunisation are available worldwide, and novel types of rotavirus vaccines are also under development, in particular, in the Russian Federation. However, there are no regulations or guidelines helping developers to design an optimal preclinical and clinical programme for rotavirus vaccines.AIM. This study aimed to analyse and summarise global experience in planning and conducting preclinical and clinical studies of rotavirus vaccines in order to provide recommendations for national vaccine developers.DISCUSSION. This study presents an analysis of the available data (and, specifically, the data obtained for the past five years) on all rotavirus vaccines used in the world that have been clinically proven to be effective in preventing severe rotavirus gastroenteritis and reducing the number of hospital admissions due to acute intestinal infections. The effectiveness of rotavirus vaccines varies in different regions of the world and may be lower in developing countries for various reasons. The safety profile of oral rotavirus vaccines is generally considered favourable. Nevertheless, there are still some concerns regarding intestinal intussusception in infants following vaccination. To address the abovementioned problems, researchers, including those in Russia, are developing novel types of rotavirus vaccines, predominantly focusing on inactivated (subunit or recombinant) preparations. For planning and conducting preclinical studies of a rotavirus vaccine, it is advisable to adopt general approaches that involve assessing the acute and chronic toxicity, immunogenicity, and safety pharmacology of the rotavirus vaccine and the virus-neutralising activity of vaccination-induced antibodies. Clinical trials of a rotavirus vaccine should assess its effectiveness in preventing rotavirus gastroenteritis of any severity, hospitalisation, and acute viral intestinal infections of any aetiology in the target age group of young children. Furthermore, it is important to confirm the safety of the rotavirus vaccine and demonstrate the absence of mutual interference with the immunogenicity of the rotavirus vaccine and other vaccines co-administered in the vaccination schedule.CONCLUSIONS. Preclinical studies of rotavirus vaccines may use standard and generally accept­ed approaches. However, planning and conducting clinical trials requires specific considerations associated with both the nature of rotavirus infection and the national infant vaccination schedule.
Viability assessment of <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Abony NCTC 6017 in ampoules and vials by the accelerated shelf-life testing method
Authors: Voropaev A. A. , Cidipova O. T. , Fadeikina O. V. , Davydov D. S. 
Published: December 2024

INTRODUCTION. A key priority in maintaining a collection of microorganisms is to ensure the stability of characteristics and the viability of microbial cultures during their storage and transport. In addition, some applications of collection strains as control samples require accurate data on the number of viable microbial cells in each sample. Therefore, it is necessary to develop and implement an analytical procedure for predicting the guaranteed shelf life of test strains.AIM. This study aimed to predict the guaranteed shelf life for test strains in a variety of primary packaging by assessing changes in microbial viability under accelerated storage conditions, with the Salmonella enterica subsp. enterica serovar Abony NCTC 6017 strain as a model organism.MATERIALS AND METHODS. The study used lyophilised samples of the S. enterica subsp. enterica serovar Abony NCTC 6017 strain deposited in the National Collection of Pathogenic Microorganisms (NCPM) at the Scientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation. The studied primary packaging types included vacuum-filled borosilicate glass ampoules and 2R lyophilisation vials. The samples were tested for a number of quality attributes (loss on drying, viable cell count, cell viability, colony morphology, and biochemical identification) and subjected to accelerated shelf-life testing at ele­vated temperatures (35–65 °С).RESULTS. The study did not show any significant differences in the quality of lyophilised samples depending on the type of primary packaging. The authors experimentally determined rate constants for the loss of viability in microbial cultures during storage at elevated temperatures and calculated the rate constants for the storage and transport temperatures and for different types of primary packaging. The predicted time to viable cell count reduction to 10% of the initial level was 19 years for vials and 25 years for ampoules, and the predicted time to 50% viability was 5.8 years for vials and 7.6 years for ampoules.CONCLUSIONS. The results of this study confirm the applicability of different primary packaging (ampoules and vials) for the lyophilisation and storage of microbial test strains. The data obtained can guide further research and contribute to the development of recommendations for the storage of lyophilised strains in various types of packaging.
Pengenalan Insektarium Sebagai Media Pembelajaran dan Entrepeneur Bagi Siswa SMA Negeri 1 Banyuasin II Sungsang
Authors: Novianti Dewi , Dian Mutiara  , Dewi Rosanti  , Kartika Trimin , Marmaini Marmaini , Warsari Dwi , Wahyudi Rizki 
Published: December 2024

Lembaga pendidikan sekolah formal, salah satunya yaitu Sekolah Tingkat Atas (SMA/SMK/MA) baik negeri dan swasta, yang dalam pelaksanaan untuk mencapai keberhasilan dalam proses belajar mengajar, saat ini mulai menerapkan program kewirausahaan (entrepreneur) bagi siswa, terutama sejak pandemic Covid-19 melanda. Kabupaten Banyuasin merupakan salah kabupaten yang terdapat di provinsi Sumatera Selatan, secara administratif terletak di kawasan pesisir Sumatera Selatan. Dalam perkembangannya, kawasan ini sudah dapat ditempuh melalui jalur darat, sehingga akses menuju ke sekolah lebih mudah. Program PkM ini bertujuan untuk memberikan informasi bagi siswa dan guru dalam pembuatan terrarium sebagai pembelajaran kewirausahaan yang didasari dari pelajaran IPA. Kegiatan dilaksanakan pada tanggal 20 November 2023 bertempat di SMAN 1 Banyuasin II Kabupaten Banyuasin. Metode pelaksanaan dilakukan dengan simulasi langsung. Hasil yang dicapai siswa dapat menerapkan teknik pembuatan insektarium sebagai penerapan pembelajaran IPA.
Peran Guru Bimbingan Konseling Dalam Meningkatkan Kemampuan Problem Solving Siswa Di MTsS Yati Kamang Mudik
Authors: Dongoran Patima , Afrinaldi Afrinaldi 
Published: December 2024

Seorang guru sangat penting untuk pembelajaran, termasuk guru BK. Guru BK sangat penting untuk proses pembelajaran di berbagai jenjang pendidikan. Guru BK di sekolah bertanggung jawab untuk membantu dan membimbing dalam pertumbuhan kepribadian dan kemampuan siswa. Tujuan dari penelitian ini untuk mengetahui Peran Guru BK Meningkatkan Kemampuan Problem Solving Siswa. Penulis Penelitian ini menggunakan metode Kualitatif Deskiptif dengan lokasi penelitian MTsS Yati Kamang Mudik. Adapun sumber data yang digunakan dalam penelitian data primer dan data sekunder. Data primer didapatkan dengan menggunakan observasi, dokumentasi dan wawancara sedangkan data sekunder didapatkan melalui perantara berupa catata, laporan perilaku dan dokumentasi yang terkait dengan tema penelitian informan kunci 1 informan utama 1 informan pendukung 5 orang siswa, maka jumlah informan keseluruhan adalah 7 orang. Dari hasil penelitian ditemukan bahwa peran guru bk dalam meningkatkan kemampuan problem solving siswa Di MTsS Yati Kamang Mudik. Adapun peran guru bk dalam meningkatkan kemampua problem solving siswa di MTsS Yati Kamang Mudik yaitu: 1. Peningkatan dalam pemecahan masalah siswa. 2 Kemandirian siswa.3 Komunikasi Kerja sama siswa 4. Ketahanan emosional siswa.
Implementasi Pembelajaran Pendidikan Pancasila Berbasis Tri Kaya Parisudha terhadap Pengembangan Karakter Siswa Sekolah Dasar
Authors: Andin Dwi Pratiwi Kadek , Rika Damayanti Kadek , Luh Putu Nia Mylinda Ni , Ngurah Ardiawan I Ketut 
Published: December 2024

The teachings of Tri Kaya Parisudha are applied in Pancasila education in elementary schools with the aim of fostering constructive thoughts, speech, and behavior in order to develop good character in students. Using descriptive qualitative method, this research was conducted at SD Negeri 4 Tegalinggah. Information was collected through literature review, interviews and observations. The findings of this study demonstrate the effectiveness of Tri Kaya Parisudha in fostering character aligned with Pancasila. It consists of three components: Manacika (thinking good), Wacika (speaking good), and Kayika (doing good). This method has been shown to improve students' good attitude both inside and outside the classroom, which contributes to the achievement of the Pancasila learner profile
Probiotic properties of <i>Limosilactobacillus </i><i>fermentum</i> strain TPGMS1 isolated from gut microflora of <i>Tai-Phake</i> community of Assam
Authors: Sarmah Minakshee , Deka Manab 
Published: December 2024

Probiotics confer health benefits in the host, such as antimicrobial activity, immune system modulation, reduce metabolic disorder and enhance anticancer activity. Most probiotic microorganisms belong to the Lactic Acid Bacteria (LAB) group. Human gut is a WHO/FAO recommended source for LAB. This study aimed to evaluate in vitro probiotic properties of LAB isolated from thehuman gut microflora of Tai-Phake community. Among 43 isolated LAB species, 26 were preliminarily selected for their antimicrobial activity. Isolates were evaluated for probiotic characteristics and antimicrobial activities against test microorganisms, Escherichia coli ATCC-10536 and Staphylococcus aureus ATCC-BAA-1026, using agar well-diffusion method. Eight of these isolates exhibited antagonistic activity against test microorganisms, which is indicated by zone of inhibition (ZoI) of 1.5 cm against Escherichia coliATCC-10536andZoI of 2.4 cm against Staphylococcus aureus ATCC-BAA-1026.The isolate with the best probiotic properties was identified using 16S rRNA sequencing as Limosilactobacillusfermentum strain TPGMS1. It exhibited high tolerance to low acidic condition (pH 2.0) and high bile salt (0.5% and 1%) concentrations, a significant hydrophobicity at 64 % and auto-aggregation of 58%. The results suggest that Limosilactobacillus fermentum strain TPGMS1 has high probiotic potential and can be further explored for probiotic formulation.
Optimization of fermentation media constituents for higher production of naringinase by <i>Paenibacillus stellifer</i> RAMCM-44 using Response Surface Methodology
Authors: Nara Rachna , Sehrawat Nirmala , Kumar Sunil , Kumar Amit , Sharma Anil Kumar , Yadav Mukesh 
Published: December 2024

Naringin is a flavonoid in citrus fruits and contributes to the bitterness of extracted citrus fruit juices. Naringinase has potential to hydrolyse the naringin into tasteless compounds and, therefore, reduces the bitterness of citrus juices. Majority of studies focused on naringinase production from fungal sources. The present study aimed to use Paenibacillus stellifer RAMCM-44 for the production of naringinase by optimization of media constituents using Response Surface Methodology (RSM) at the shake-flask level. Five media components were taken as variables with different concentrations to determine the optimal concentrations of media components. The five variables included peptone (0.5-1%, w/v), yeast extract (0.4-0.8%, w/v), NaCl (0.5-1%, w/v), naringin (0.1-0.25%, w/v) and KH2PO4 (0.5-1%, w/v), while MgSO4 (0.05%, w/v), MnSO4 (0.001%, w/v) and initial pH (6.5±0.2) during shake-flask fermentation, media were kept constant. The experimental data was analysed (RSM) and optimal levels of peptone (0.75%), yeast extract (0.6%), NaCl (0.75%), naringin (0.17%) and KH2PO4 (0.75%) were determined. The experimental values of naringinase activity (8.55 IU/ml) and biomass (0.826 OD600) closely matched the predicted values. The criteria, including analysis of variance (ANOVA), R values, coefficient of variance and model significance were assessed. The R-value for naringinase production was found to be 0.9976, while the model F-value was 228.74 and the lack of fit F-value was 109.77 for naringinase activity, revealing the current study's significance. This is the first report on optimising media components and applying RSM for the production of naringinase by P. stellifer RAMCM-44, which could benefit studies involving scale-up.
A study on elevated carbon dioxide (eCO<sub>2</sub>), elevated temperature (eT) and their interactive effect on chickpea (<i>Cicer arietinum</i> L.) yield and seed mineral nutrients
Authors: Maurya Sadhna , Chopra A. K. 
Published: December 2024

Continuously increasing carbon dioxide concentration is predicted to elevate the earth’s temperature. Elevated temperature is a severe problem for the cultivation of chickpea (Cicer arietinum L ). Therefore, the present study aimed to assess the effect of elevated carbon dioxide (eCO2), elevated temperature (eT) and their interactive effect on yield and seed mineral nutrients of two genotypes, i.e., ICC 4958 (desi) and Flip 90-166 (kabuli) of chickpea (C. arietinum L ). The pot experiments were conducted in Open Top Chamber (OTC) for two consecutive years (2019-20 and 2020-21), along with the control placed in ambient natural conditions. The eCO2 (650±50 µl/l) and eT (~4oC) were given individually and in combination. The gaseous exchange was measured at the flowering stage. After harvesting, yield and its parameters, seed protein and mineral nutrients were determined using standard methods. Under eCO2, the photosynthesis of both genotypes was positively affected, ultimately converting to yield (8.8-17.5% increase). However, the effect was more prominent in ICC 4958 than Flip 90-166. Higher temperature only positively affected dry biomass, but that effect was not converted to yield; instead, a reduction occurred in yield (- 12.0 to -26.9% ). In combination with eCO2 and eT, the negative effect of high temperature was ameliorated by eCO2 on yield, augmenting the effects on seed nutrient reduction. Among the seed mineral nutrients, Na, K, Fe and Zn were most reduced (-20.3 to -30.0%) under interactive effect. The findings will help to enhance seed yield with improved mineral nutrient content of chickpea.
ПОСТМАЙНІНГОВІ ЛАНДШАФТИ ЛЬВІВСЬКО-ВОЛИНСЬКОГО КАМ’ЯНОВУГІЛЬНОГО БАСЕЙНУ, ЇХ ФОРМУВАННЯ І РОЗВИТОК
Authors: Іванов Євген Анатолійович , Андрейчук Юрій Михайлович , Пилипович Ольга Василівна 
Published: December 2024

Розглянуто питання виникнення, формування і розвитку постмайнінгових ландшафтів у Львівсько-Волинському кам’яновугільному басейні. Закриття нерентабельних вугільних шахт прискорило появу на заміну вже сформованим гірничопромисловим ландшафтам нових геосистем природно-антропогенної генези на території промислових майданчиків та в межах шахтних полів. На прикладі двох модельних ділянок досліджено особливості функціонування постмайнінгових геосистем, які утворилися на літологічній основі вугільних відвалів та у зонах затоплення, підтоплення і вторинного заболочення природних місцевостей. Проведено геоінформаційне картографування і моделювання екологічного стану об’єктів дослідження у масштабі 1 : 2 000 – 1 : 5 000. Запропоновано оптимізаційні заходи щодо покращення екологічного стану постмайнінгових геосистем та раціонального використання природних ресурсів.
РЕЛІГІЙНИЙ ТУРИЗМ І САКРАЛЬНА ПРИВАБЛИВІСТЬ ЛАНДШАФТІВ: ДО ІСТОРІЇ ТЕОРІЇ
Authors: Божук Тетяна Іванівна 
Published: December 2024

Значна кількість релігійних об’єктів на території України, що відзначається різноманіттям ландшафтів, з їх унікальними сакральними цінностями стали предметом дослідження і сприяли становленню та розвитку географії релігій, а згодом і релігійного туризму, що сформувало фундамент для всіх подальших досліджень у цьому напрямку. Саме тому, мета проведеного дослідження полягала у простеженні таких змін шляхом аналізу наукових публікацій вітчизняних та іноземних географів і не географів впродовж 1990-2010 рр. Представлена публікація носить оглядовий характер; розглянуто розуміння і систематизацію дефініцій «географія релігій» і «релігійний туризм» у працях вітчизняних та іноземних науковців. Проведений аналіз має наукову цінність і може бути використаний для розробки теоретико-методичних цілей дослідження у галузі географії релігій і релігійного туризму.
ФОРМУВАННЯ ПРОФЕСІЙНОЇ ГОТОВНОСТІ КЕРІВНИКА КАФЕДРИ МИСТЕЦЬКОГО СПРЯМУВАННЯ ЗАКЛАДУ ВИЩОЇ ОСВІТИ ДО ВИКОНАННЯ ОРГАНІЗАТОРСЬКОЇ ФУНКЦІЇ
Authors: Овчаренко Наталія 
Published: December 2024

The article is about the solution of the actual problem of pedagogical science such as the formation of the professional readiness in the head of the department in artistic direction of the institution in higher education to perform the organizational function.
There is an urgent need for the training of management personnel to institutions of higher education who adhere to the new humanistically oriented philosophy of education, who are able to think strategically, take responsibility, act independently of conditions in uncertainty, have a creative attitude to activities, self-actualize themselves and the team for continuous improvement and development.
The purpose of the article is to substantiate the theoretical aspects of the formation of the professional readiness in the head of the department in artistic direction of the institution in higher education to perform the organizational function. It is determined that the professional readiness in the head of the department of the higher education institution to perform the organizational function is a personal and professional education, in accordance with the goal and set tasks. There are the results of the professional training of the head and components of his professional competence, a system of organizational values, knowledge, skills, abilities that manifest themselves of the ability to perform organizational activities of a team.
The specificity of the organizational function in the head of the department in artistic direction of a higher education institution is to achieve high efficiency of educational, scientific, methodical, organizational work of the department in combination with the organization of artistic and creative activities. There is to achieve distribution of work between members of the department the set goals and implement plans; directing, instructing and controlling the activities of team members; ensuring positive communication in the work of the department and the educational process; creating a creative atmosphere.
If the following pedagogical conditions are ensured in the educational institution such as stimulation of the positive motivation in the head of the department in artistic direction of the institution in higher education to increase the level of organizational culture; creating of an informational and educational environment improve the formation of the professional readiness in the head of the department in artistic direction of the institution in higher education to perform the organizational function will take place more effectively in a higher education institution for professional training of the head of the department for organizational activities. It`s use of innovative communication technologies, trainings aimed at self-improvement, self-development in the head of the department of artistic direction in the higher education institution to perform the organizational function.