bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Clinical Randomized Controlled Trial: Moxibustion at Laogong Interval With Panax Notoginseng Promoted the Maturation of Arteriovenous Fistulae
Authors: Chen Yurou , Xu Lin , Huang Di , Chen Dongping , Wu Feng , Wang Luobing , Zhou Jie , Lan Tianying , Qin Xuehua , Ye Chaoyang 
Published: October 2021

Abstract
Background: We aim to study the clinical effect of moxibustion at Laogong interval with Panax notoginseng on the short-term maturation and long-term patency of arteriovenous fistula. Methods: Seventy-four pre-dialysis uremic patients who received radial artery-cephalic vein fistula creations were enrolled in this study and randomly assigned to the control group and experimental group. After arteriovenous fistula creations, the control group underwent handgrip exercise, and the experimental group received moxibustion at Laogong acupoint interval with Panax notoginseng. Patients were treated for 12 weeks and followed up for 24 weeks at the following time points: before creations and 2, 4, 8, 12, 24 weeks after creations. The diameter of anastomosis, the diameter of draining-veins, the blood flow of draining-veins, the diameter of arteria brachialis, and the blood flow of arteria brachialis evaluated the maturation and patency of arteriovenous fistula. ELISA determined serum levels of ET and NO levels.Results: The maturity rate in the experimental group was significantly higher than that in the control group at 4 weeks after arteriovenous fistula creations (P =0.048). The diameter of anastomosis, the diameter of draining veins, and the blood flow of draining veins increased in both groups during the whole 24 weeks. The diameter and blood flow of arteria brachialis ascended in both groups during the previous 12 weeks. Compared with the control group, moxibustion at Laogong interval with Panax notoginseng significantly improved the value of the diameter of draining-veins (P =0.016), the blood flow of draining-veins (P = 0.015), the diameter of arteria brachialis (P < 0.001), and the blood flow of arteria brachialis (P =0. 012) at 2 weeks, and enhanced the blood flow of draining-veins (P =0.029) and arteria brachialis (P < 0.001) at 12 weeks. Serum levels of ET were significantly lower (P =0.047), and serum levels of NO were markedly higher (P < 0.001) in the experimental group than that in the control group at 2 weeks after creations. Conclusion: Moxibustion at Laogong interval with Panax notoginseng was non-invasive and promoted the maturation of arteriovenous fistula at 4 weeks after creations. However, its long-term beneficial effect on patency after creations was not significant.Trial registration: Chinese Clinical Trial Registry, No.ChiCTR1900024042. Registered, http://www.chictr.org.cn/index.aspx.
Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Wen-yu-jin Against Pulmonary Fibrosis in Mouse Model
Authors: wang lu , Zhu Wenxiang , Sun Rui , Liu Jing , Ma Qihong , Zhang Binbin , Shi Yuanyuan 
Published: October 2021

Abstract
Background
Pulmonary fibrosis (PF) is a devastating lung disease. The two drugs approved by the FDA, pirfenidone and nintedanib, can only delay the progression of the disease but cannot cure the disease. These drugs also present adverse effects. Wen-yu-jin (WYJ) obtained from steamed roots of Curcuma wenyujin showed a variety of pharmacological activities. In this study we investigated whether WYJ present anti-lung fibrosis effects.
Methods
Ultra-high pressure liquid chromatography combined with linear ion trap-orbital tandem mass spectrometry (UHPLC-LTQ-orbital trap) was used to identify chemical composition of WYJ. PF-related and WYJ-related targets were obtained from public databases. Network pharmacological was performed to acquire potential targets and major signaling pathways. The binding activity of composition with core targets was predicted by molecular docking. Based on the predicted results, the anti-lung fibrosis effect of WYJ was verified in vivo and in vitro experiments.
Results
23 major compositions of WYJ were identified based on UHPLC-LTQ-Orbitrap. According to the results of network pharmacology, MAPK signaling pathway might play an important role in WYJ against lung fibrosis and STAT3 also could be the potential therapeutic targets. Molecular docking results indicated that most of the compositions have good binding activities with core targets. In vivo and in vitro experiments showed that WYJ alleviated process of fibrosis by inhibiting MAPK signaling pathway and the levels of phosphorylated STAT3 (p-STAT3).
Conclusion
According to the results of network pharmacology and molecular docking, in vivo and in vitro experiments further verified potential targets and molecular mechanism of WYJ against lung fibrosis. Our study provided a novel approach to explain the pharmacological basis of other herbs.
Revealing the Therapeutic Targets and Mechanism of Baicalin for Anti-chronic Gastritis Through TMT Proteomic Approach
Authors: Ji Wanli , Huo Yan , Zhang Yu , Wang Xinhong , Zhang Yifan 
Published: October 2021

Abstract
BackgroundBaicalin (BCL) is a natural compound with beneficial activities, including antioxidant, anti-inflammatory and immunomodulatory. To investigate the therapeutic action of baicalin treatment in ethanol-induced chronic gastritis. Here, we investigated the proteome changes in the gastric tissue to elucidate the therapeutic targets of baicalin in chronic gastritis by TMT-based quantitative proteomics.ResultsUsing TMT-based quantitative proteomics, a total of the 4,452 proteins were identified and quantified in the gastric antrum tissue of Sprague–Dawley rats. Of these, 107 differentially expressed proteins, including 44 up-regulated and 63 down-regulated proteins, were uncovered in the baicalin-treated group as compared with the untreated group with ethanol-induced gastritis. Furthermore, the expression of TPM2, GIMAP4, and Mpc1 was validated using Western Blot. Baicalin could decrease the production of interleukin (IL)-2, IL-8 and tumor necrosis factor-α (TNF-α), while increase the expression of epidermal growth factor (EGF) and B-cell lymphoma-2 (Bcl-2). Notably, protein-protein interaction network analysis revealed the widespread interactions mediated by baicalin.ConclusionsWe investigated the effects and potential mechanism of baicalin in chronic gastritis. Proteomic technology was used to explore baicalin-affected proteins and some signaling pathways. The results may provide important insights into the discovery of potential target proteins for the treatment of chronic gastritis.
Impacts of eosinophil percentage on prognosis acute type A aortic dissection patients
Authors: Shao Yue , Ye Liu , Shi Hao-ming , Wang Xin-mei , Luo Jun , Liu Lu , Wu Qing-chen 
Published: October 2021

Abstract
Background
Eosinophils are pro-inflammatory cells involved in thrombosis and have been proposed as a prognosis marker in acute ischemic stroke and ST-elevation myocardial Infarction. Here, we sought to clarify the prognostic value of eosinophil percentage (EOS%) in patients with acute type A aortic dissection (AAAD).
Methods
We examined 183 consecutive AAAD patients. Based on the optimum cut-off value of EOS% determined by X-tile software, patients were classified into the low EOS% (EOS%≤0.1) and high EOS% groups (EOS%>0.1). We performed multivariate regression analysis and Kaplan-Meier (KM) survival curves to assess the association between EOS% and mortality. Eosinophil accumulation in aortic dissection intraluminal thrombus was confirmed using hematoxylin–eosin (H&E) staining. An external cohort from Medical Information Mart for Intensive Care IV was performed to validate the results.
Results
Relative to surviving patients, those who died during hospitalization had significantly lower EOS% (p=0.001) but significantly higher WBC (p=0.002) and neutrophil (p=0.001) counts. Multivariate regression analysis identified EOS% as an independent predictor of in-hospital and 1-year mortality. KM curves revealed that 1-year cumulative mortality was significantly higher in the low EOS% group, although it was mainly attributed to the higher 30-day mortality. H&E staining revealed massive infiltration of eosinophils in all 20 thrombus specimens. The external validation confirmed that relative to survivors, patients with in-hospital (p=0.010) had significantly lower EOS%. Moreover, multivariate regression analyses identified that decreased EOS% was independently significantly associated with in-hospital mortality.
Conclusions
Low EOS% is significantly related to increased mortality rates in AAAD patients.
Polygonum Barbatum Extract Reduces Colorectal Cancer Cell Proliferation, Migration, Invasion, and Epithelial-mesenchymal Transition via Regulation of the YAP and β-catenin Pathways
Authors: Chang Pi-Kai , Yen I-Chuan , Tsai Wei-Cheng , Lee Shih-Yu 
Published: October 2021

Abstract
BackgroundColorectal cancer (CRC) is the third most common cancer worldwide, but the development of novel therapeutics for CRC remains a challenge. Polygonum barbatum has anticancer potential, but its mechanism of action requires further investigation. This study was designed to investigate the inhibitory effect of Polygonum barbatum on human CRC cells. The HPLC fingerprints of the Polygonum barbatum extract (PBE) and quercetin standard were determined using analytical RP-HPLC and evaluations were completed using the human colon cancer cell line HCT-116 (KRASG13D mutation) and HT-29 cells. After treatment with PBE, cell viability, colony formation, migration, invasion, and apoptosis were analyzed using CCK-8, colony formation, wound healing, Transwell invasion, and flow cytometry assays, respectively. RNA-sequencing, western blotting, and co-immunoprecipitation were also used to analyze changes in the whole-transcriptome of these cells and identify possible mechanisms of action for PBE in CRC cells. ResultsPBE significantly reduced CRC cell growth, migration, and invasion, and Gene Ontology analysis showed that the genes responsible for extracellular matrix organization, cell motility, and cell growth were suppressed by PBE. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the differentially expressed genes revealed that PBE treatment exerted a significant effect on the extracellular matrix interaction and focal adhesion pathways. Consistently, epithelial-to-mesenchymal transition markers, including N-cadherin, vimentin, SLUG, and SNAIL, were also all shown to be regulated by PBE. These effects were associated with blockade of the Yes-associated protein and the GSK3β/β-catenin axis.Conclusion﻿Polygonum barbatum extract exerts a significant inhibitory effect on CRC cells and may be potentially applicable in clinical trials.
Evidence-Related Requirements in Swedish Public Sector Procurement of Health and Welfare Technologies – A Systematic Review
Authors: Richardson Matt X. , Stridsberg Sara Landerdahl , Wamala-Andersson Sarah 
Published: October 2021

Abstract
BackgroundHealth and welfare technologies (HWT) are becoming increasingly employed in the Nordic countries, and in Sweden in particular. The amount of HWT public procurement is likely increasing at a similar rate, but requirements for evidence of effectiveness placed on bidders during this process may be lacking. Method This study investigated the use of evidence as a requirement in public sector tendering process of HWT, and how it affected bidder attributes and procurement outcomes. A novel type of systematic review and content analysis of requests for tenders for HWT announced prior to June 2021 was therefore conducted in Swedish public procurement databases.ResultNinety requests for tenders for 11 types of HWT met the inclusion criteria for review, accounting for potential contracts worth 246 to 296 million EUR. Criteria requiring evidence for effectiveness were used in 16 requests for tenders, accounting for 183 million EUR in potential contracts. Eight of the requests referred to an established independent standard to confirm such evidence, such as CE standard of conformity, MDR and/or MDD. This prevalence appears to cut across all types of procuring organisations and all types of HWT. The use of any evidence criteria, or lack thereof, does not appear to affect the outcomes of the tendering process. Conclusion Criteria requiring evidence for effectiveness are used in less than a fifth of all public procurements of health- and welfare technologies in Sweden, and less than 10 % refer to some form of independent standard as confirmation of such evidence. The procurement process therefore risks creating a legacy of sub-optimal technologies in health- and social care services. More prevalent and specific requirements for evidence and its continual generation in the procurement process are highly recommended. Recommendations for decision makers, procurement managers, and developers are made.
A Retrospective Longitudinal Cohort Study of The Clinical Burden in Myasthenia Gravis
Authors: Harris Linda , Graham Sophie , MacLachlan Sharon , Exuzides Alex , Jacob Saiju 
Published: October 2021

Abstract
Background

Patients with generalized myasthenia gravis (MG) often experience debilitating exacerbations, with the possibility of life-threatening respiratory crises requiring hospitalization. Long-term longitudinal studies are needed to understand the burden of MG, including in patients whose disease is refractory to conventional treatment.
Methods

A retrospective, longitudinal, cohort study was conducted of patients in England aged ≥18 years with treatment-refractory or non-refractory MG, using data recorded during 1997–2016 in the Clinical Practice Research Datalink and the Hospital Episode Statistics databases. A control cohort of patients without MG, matched to the patients in the treatment-refractory MG cohort, was also identified. Outcome measures included myasthenic crises, MG exacerbations, MG-related hospitalizations, comorbidities, and all-cause mortality. Descriptive statistics were calculated for the overall MG population. For continuous variables, between-cohort comparisons were made using t tests for normally distributed data and Mann–Whitney U tests for non-normally distributed data. For categorical data, the comparisons were made by chi-squared tests. Differences in clinical outcomes between cohorts were modeled using negative binomial regression.
Results

A total of 1149 patients with MG were included. Overall, 18.4% of patients experienced myasthenic crises, 24.6% experienced exacerbations, and 38.6% underwent MG-related hospitalizations. Most of these events occurred within 2–3 years of diagnosis. Patients with MG refractory to conventional treatment (n=66) experienced more exacerbations and MG-related hospitalizations than patients with non-refractory disease (n=1083). Patients with refractory MG experienced a higher frequency of renal disease and hypertension compared with patients with non-refractory MG, and with matched patients without MG. They were also more likely to have diabetes and congestive heart failure than the matched controls. Rates of all-cause mortality during the follow-up period did not differ between patients with refractory MG and non-refractory MG.
Conclusions

These results show that conventional treatments for MG are not adequately managing patients’ symptoms and that patients with refractory MG are more likely to experience certain comorbidities than those with non-refractory MG or matched controls without MG. Future research should focus on the impact of newer targeted therapies on long-term clinical outcomes and comorbid conditions.
A Promising New Predictive Factor for Detecting Bowel Resection in Childhood Intussusception: Lymphocyte-C Reactive Protein Ratio
Authors: Chen Bailin Chenilin , Cao Jian , Yan Chengwei , Zheng Chao , Chen Jingyu , Guo Chunbao 
Published: October 2021

Abstract
Background: The most critical concern for management of childhood intussusception is the bowel resection due to the intestinal ischemia and necrocis. It is of great importance for early prediction of this problem. We investigate the value of various combinations of inflammatory factors to predict the intestinal necrocis and resection.Methods: We retrospectively reviewed the medical records of pediatric patients with intussusception, which undergone surgical management. During the research period, 47 patients undergone intestinal resection due to intestinal necrocis and 68 patients without intestinal resection were enrolled. We evaluated the diagnostic values of various combination of inflammatory markers from preoperative period laboratory analyses using the receiver operating characteristic (ROC) method.Results: In the current cohort, 115 patients were operated for intussusception, among them, 47 patients (40.9%) undergone intestinal resections. In patients with intestinal resections, neutrophil count(p=0.013), CRP(p=0.002), platelet–lymphocyte ratio(PLR, p=0.008), NLR(neutrophil–lymphocyte ratio, p=0.026), and LCR(lymphocyte–CRP ratio, p<0.001) values were significantly higher than those in the patients without any resections. Receiver operating characteristic (ROC) analysis results showed that the combination of lymphocytic count along with C-reactive protein levels (LCR) demonstrated the highest correlation with intestinal resection due to intussusception compared with other parameters in patients, with sensitivity of 0.82(0.73–0.86) and specifficity of 0.80(0.57–0.94) for the diagnosis of strangulation. Conclusion: The preoperative LCR level is a useful marker to predict the need for intestinal resection due to intestinal necrosis in patients with intussusception.
Transurethral Incision of the Bladder Neck with Additional Procedure Resumes Spontaneous Voiding in Female Voiding Dysfunction – A Long-term Follow-up
Authors: Ong Hueih Ling , Kuo Hann-Chorng 
Published: October 2021

Abstract
Introductions: To evaluate the long-term effectiveness of transurethral incision of the bladder neck (TUI-BN) with or without additional procedure for female voiding dysfunction.Methods: Women with voiding difficulty and underwent TUI-BN in recent 12 years were included. All patients underwent videourodynamics study (VUDS) at baseline and after TUI-BN. Successful outcome was defined as having a voiding efficiency (VE) by ≥50% after treatment. Patients with insufficient improvement were opt for repeated TUI-BN, urethral onabotulinumtoxinA injection or transurethral external sphincter incision (TUI-ES). The current voiding status, surgical complications were evaluated.Results: A total of 102 women with VUDS evidence of a narrow bladder neck during voiding were enrolled. The long-term success rate of the first TUI-BN was 29.4% (30/102) and increased to 66.7% (34/51) after combining TUI-BN and additional procedure. The overall long-term success rates were 74.6% in detrusor underactivity (DU), 52.0% in detrusor overactivity and low contractility, 50.0% in bladder neck obstruction (p=0.022). Spontaneous voiding was achieved in 66 (64.7%) patients, de novo urinary incontinence in 21 (20.6%), and vesicovaginal fistula in 4 (3.9%), all were repaired.Conclusions: TUI-BN alone or in combination with additional procedure was safe, effective and durable. Patients with DU benefit most in resuming spontaneous voiding.
Parameters in complete blood cell count and coagulation function test predict in pediatric critical illness：A retrospective study
Authors: Hang Hang , Zhao Wu , Guo Qixiu 
Published: October 2021

Abstract
Background. The complete blood cell count is associated with the prognosis of critically ill children. Pediatric critical illness score (PCIS) is often used to evaluate the criticality of pediatric critical illness. We aimed to determine most parameters in the complete blood cell count (CBC) and coagulation function test of patients who were admitted to PICU in our hospital and compare those parameters of critically ill and non-critically ill children grouped by PCIS and whether CBC can identify and assess the severity of illness in critically ill children. Methods. We performed a retrospective study of pediatric intensive care population who took both blood routine examination and coagulation function test within 24 hours of admission in PICU of The First Affiliated Hospital of Bengbu Medical College, from April 2015 to December 2019. Data collected include patient demographics and clinical data. Statistical analysis including Mann-Whitney U Test, binary logistic regression and so on. Results. The parameters in the complete blood cell count and coagulation function test showed a significant difference between critical illness and non-critical illness in this study. Multivariate logistic regression analysis showed that RDWSD and PT prolongation are associated with PCIs (p<0.001, p=0.006, respectively). We created a nomogram for pediatric critical illness by using these risk factors. The area under of ROC curve (AUC) of pediatric critical illness for the combination of RDWSD and PT prolongation was 0.664(95%confidence interval 0.609-0.720). The nomogram had a bootstrapped-concordance index of 0.58. Conclusion. RDWSD and PT prolongation are reliable indicators for predicting pediatric critical illness.
Research on Forecast of Global COVID-19 Development Trend
Authors: Cheng Yunyun , Bai Yanping , Xu Ting , Cheng Rong , Hu Hongping 
Published: October 2021

Abstract
The rapid spread of Corona Virus Disease 2019 (COVID-2019) has seriously threatened people’s health and brought huge challenges to the medical systems of many countries. So it is necessary to predict the epidemic trend scientifically and accurately. In this paper, the development trend prediction of the global daily cumulative confirmed case, based on data from February 2, 2020 to August 14, 2021 using linear regression and nonlinear regression methods, which are auto regressive integrated moving average (ARIMA), wavelet neural network (WNN), support vector machine (SVM), recurrent neural network (RNN) and long short-term memory (LSTM). Mean Absolute Error (MAE), Root Mean Squared Error (RMSE) and Mean Absolute Percentage Error (MAPE) were used to evaluate the prediction accuracy of the five models. The experimental results showed that the LSTM used in this study is more accurate for predicting the development trend of global cumulative confirmed cases. And its three evaluation indexes were 0.0936%, 0.1123%, and 0.0962% respectively, which were the smallest compared with the other four models. The LSTM model was used to predict the global cumulative confirmed cases in the next five days. The prediction results showed that the global cumulative confirmed cases will rise steadily and exceed 205 million. Therefore, the health departments of various countries should take appropriate prevention and control measures in advance.