bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eradication of Klebsiella quasipneumoniae by Eudragit-formulated klebicin KvarIa in the intestinal tract of mice
Authors: Karaliute Indre , Ramonaite Rima , Bernatoniene Jurga , Petrikaite Vilma , Misiunas Audrius , Denkovskiene Erna , Razanskiene Ausra , Gleba Yuri , Kupcinskas Juozas , Skieceviciene Jurgita 
Published: November 2021

Abstract
Background

Klebsiella quasipneumoniae is an opportunistic pathogen causing antibiotic-resistant infections of the gastrointestinal tract in many clinical cases. Orally delivered bioactive Klebsiella-specific antimicrobial proteins, klebicins, could be a promising method to eradicate Klebsiella species infecting the gut.
Methods

Mouse infection model wasestablished based on infection of streptomycin treated BALB/C mice with K. quasipneumoniae strain DSM28212. Four study groups were used (3 animals/group) to test the antimicrobial efficacy of orally delivered klebicin KvarIa: vehicle-only group (control, phosphate-buffered saline), and other three groups with bacteria, antibiotic therapy and 100 µg of uncoated Kvarla, 100 µg coated KvarIa, 1000 µg coated-KvarIa. Because of the general sensitivity of bacteriocins to gastroduodenal proteases, Karla doses were coated with Eudragit®, a GMP-certified formulation agent that releases the protein at certain pH. The coating treatment was selected based on measurements of mouse GI tract pH. The quantity of Klebsiella haemolysin gene (khe) in faecal samples of the study animals was used to quantify the presence of Klebsiella.
Results

GI colonization of K. quasipneumoniae was achieved only in the antibiotic-treated mice groups. Significant changes in khe marker quantification were found after the use of Eudragit® S100 formulated klebicin KvarIa, at both doses, with a significant reduction of K. quasipneumoniae colonization compared to the vehicle-only control group.
Conclusions

Mouse GI tract colonization with K. quasipneumoniae can be achieved if natural gut microbiota is suppressed by prior antibiotic treatment. The study demonstrates that GI infection caused by K. quasipneumoniae can be significantly reduced using Eudragit®-protected klebicin KvarIa.
The New Useful High-Resolution Computed Tomography Finding for Diagnosing Fibrotic Hypersensitivity Pneumonitis: “Hexagonal Pattern”: A Single-Center Retrospective Study
Authors: Okabayashi Hiroko , Fukuda Taiki , Iwasawa Tae , Oda Tsuneyuki , Kitamura Hideya , Baba Tomohisa , Takemura Tamiko , Sakagami Takuro , Ogura Takashi 
Published: November 2021

Abstract
Background
Centrilobular nodules, ground-glass opacity (GGO), mosaic attenuation, air trapping, and three-density pattern were reported as high-resolution computed tomography (HRCT) findings characteristic of fibrotic hypersensitivity pneumonitis (HP). However, it is often difficult to differentiate fibrotic HP from idiopathic pulmonary fibrosis (IPF). In fibrotic HP, the HRCT sometimes shows tortoiseshell-like interlobular septal thickening that extends from the subpleural lesion to the inner layers. This finding is called “hexagonal pattern,” and this study is focused on the possibility that such finding is useful for differentiating fibrotic HP from IPF.
Methods
This study included patients with multidisciplinary discussion (MDD) diagnosis of fibrotic HP or IPF undergoing surgical lung biopsy between January 2015 and December 2017 in Kanagawa Cardiovascular and Respiratory Center. Two radiologists have evaluated the HRCT findings without clinical and pathological information.
Results
A total of 23 patients were diagnosed with fibrotic HP by MDD and 48 with IPF. Extensive GGO, centrilobular nodules, and hexagonal pattern were more frequent findings in fibrotic HP than in IPF. No significant difference was observed between the two groups in the presence or absence of mosaic attenuation, air trapping, or three-density pattern. In the multivariate logistic regression, the presence of extensive GGO and hexagonal pattern was associated with increased odds ratio of fibrotic HP. The sensitivity and specificity of the diagnosis of fibrotic HP in the presence of the hexagonal pattern were 69.6% and 87.5%, respectively.
Conclusion
Hexagonal pattern is a useful finding for differentiating fibrotic HP from IPF.
Comparative longitudinal variation of total, IgG and IgA anti-SARS-CoV-2 antibodies in recipients of BNT162b2 vaccination
Authors: Salvagno Gian Luca , Henry Brandon M. , Pighi Laura , De Nitto Simone , Gianfilippi Gianluca , Lippi Giuseppe 
Published: November 2021

Abstract
Background: This article aims to summarize the 6-month variation of a vast array of anti-SARS-CoV-2 antibodies in recipients of BNT162b2 mRNA-based vaccination.Methods: The study population consisted of 84 baseline SARS-CoV-2 seronegative healthcare employees (median age 45 years, 53.6% females), receiving mRNA-based BNT162b2 vaccination. Blood was collected before the first and second BNT162b2 vaccine doses, as well as 1, 3 and 6 months afterwards. The serum titers of the following anti-SARS-CoV-2 antibodies were assayed: total anti-RBD, anti-spike trimeric IgG, anti-RBD IgG and anti-spike S1 IgA.Results: All antibodies levels peaked 1 month after vaccination, but then displayed a considerable decrease. The median rates of 6-month decline were -95% for IgG anti-SARS-CoV-2 RBD, -85% for IgG anti-SARS-CoV-2 trimeric spike, -73% for IgA anti-SARS-CoV-2 S1 and -56% for total anti-SARS-CoV-2 RBD antibodies, respectively. The median time of seronegativization was estimated at 579 days for total anti-SARS-CoV-2 RBD antibodies, 271 days for IgG anti-SARS-CoV-2 trimeric spike, 264 days for IgG anti-SARS-CoV-2 RBD and 208 days for IgA anti-SARS-CoV-2 S1, respectively. The rate of seropositive subjects declined from 98-100% at the peak to 50-100% after 6 months. The inter-individual variation of anti-SARS-CoV-2 antibodies reduction at 6 months was 3-44% from the peak.Conclusions: The results of this longitudinal serosurvey demonstrate that the titer of anti-SARS-CoV-2 antibodies declined 6 months after BNT162b2 vaccination, with median time of IgG/IgA seronegativization estimated between 7-9 months, thus supporting the opportunity of administering vaccine boosters approximately 6 months after the last dose.
A COVID-19 Mortality Prediction Model for Korean Patients Using Nationwide Korean Disease Control and Prevention Agency Database
Authors: Jee Yongho , Kim Yi-Jun , Oh Jongmin , Kim Young-Ju , Ha Eun-Hee , Jo Inho 
Published: November 2021

Abstract
The experience of the early nationwide COVID-19 pandemic in South Korea had led to an early shortage of medical resources. For efficient resource allocation, accurate prediction for the prognosis or mortality of confirmed patients is essential. Therefore, the aim of this study was to develop an accurate model for predicting COVID-19 mortality using epidemiolocal and clinical variables and for identifying high risk group of confirmed patients. Clinical and epidemiolocal variables of 4,049 patients with confirmed COVID-19 between January 20, 2020 and April 30, 2020 collected by Korean Disease Control and Prevention Agency were used. Among 4,049 total confirmed patients, 223 patients were dead while 3,826 patients were released from isolation. Patients who had the following risk factors showed significantly higher risk scores: age over 60 years, male, difficulty breathing, diabetes, cancer, dementia, change of consciousness, and hospitalized in intensive care unit. High accuracy was shown for both the development set (n = 2,467) and the validation set (n = 1,582), with AUC of 0.96 and 0.97, respectively. The prediction model developed in this study based on clinical features and epidemiological factors could be used for screening high risk group of patients and for evidence-based allocation of medical resources.
Non-mercaptalbumin is Significantly Associated with the Coronary Plaque Burden and the Severity of Coronary Artery Disease
Authors: Chou Shengpu , Yasukawa Keiko , Fujino Yusuke , Ishibashi Midori , Haraguchi Mikiko , Sato Masaya , Ikeda Hitoshi , Nakamura Sunao , Yatomi Yutaka 
Published: November 2021

Abstract
Human non-mercaptalbumin (HNA), oxidized form of serum albumin, has been reported as a useful marker in oxidative stress-related diseases; however, few reports have examined the association between HNA and the severity of coronary artery disease (CAD). The present study evaluated whether the HNA fraction is correlated with coronary artery stenosis in 140 patients considered to have a high risk of CAD or who were suspected of having acute coronary syndrome. The severity of CAD was defined by the number of stenotic coronary vessels and a severity score system (the Gensini score). HNA measurements were performed using our newly established high-performance liquid chromatography methodology. The results had shown that HNA was significantly increased in patients with three-vessel disease, compared with those without CAD or with single-vessel disease (p=0.025), and was positively correlated with the Gensini score (ρ=0.421, p<0.001). A multivariate analysis showed that the number of stenotic vessels was an independent and significant factor associated with HNA (ρ=1.246, p=0.012). A logistic regression analysis showed that HNA was a strong predictor of multivessel CAD (odds ratio, 1.12; 95% confidence interval, 1.020-1.229; p=0.017). These findings indicate that the measurement of HNA could be clinically practical for predicting the severity of coronary artery stenosis.
Water Security: A Geospatial Framework for Urban Water Resilience
Authors: Tholiya Jyoti Jain , Chaudhary Navendu 
Published: November 2021

Abstract
Urban water issues impacting sustainable development can be analyzed, modeled, and mapped through cutting-edge geospatial technologies; however, the water sector in developing countries suffers various spatial data-related problems such as limited coverage, unreliable data, limited coordination, and sharing. Available spatial data is limited to the aggregate level (i.e., National, State, and District level) and lacks details to make informed policy decisions and allocations. Despite significant advancements in geospatial technologies, its application and integration at the policy and decision-making level are seldom. The current research provides a unique, holistic Geospatial Framework to measure and monitor water security through geospatial technologies. The study demonstrates the application of the proposed Geospatial Framework from technical and institutional perspectives in water-stressed zones in Pune city showing where and how to solve problems and where proposed actions can have the most impact on creating a sustainable water-secured future. The research encourages cross-disciplinary collaboration, decentralized activity, employing traditional and indigenous knowledge, green infrastructure, watershed management, and nature-based solutions through Geospatial Framework to solve the primary challenges of water and build our cities' resilience. The current research can collaborate with Municipal Corporation mutually beneficial and work towards open-linked geospatial data for water security.
Enhancement of Electrocatalytic Oxygen Evolution by Chiral Molecular Functionalization of Hybrid 2D Electrodes
Authors: Liang Yunchang , Banjac Karla , Martin Kévin , Zigon Nicolas , Lee Seunghwa , Vanthuyne Nicolas , Garcés Felipe Andrés , Galan-Mascaros Jose Ramon , Hu Xile , Avarvari Narcis , Lingenfelder Magalí 
Published: November 2021

Abstract
A sustainable future requires highly efficient energy conversion and storage processes, where electrocatalysis plays a crucial role. The activity of an electrocatalyst is governed by the binding energy towards the reaction intermediates, while the scaling relationships prevent the improvement of a catalytic system over its volcano-plot limits. To overcome these limitations, unconventional methods that are not fully determined by the surface binding energy can be helpful. Here, we use organic chiral molecules, i.e., hetero-helicenes, to boost the oxygen evolution reaction (OER) by ca. 131.5% (at the potential of 1.65 V vs. RHE) at state-of-the-art 2D catalysts via a spin-polarization mechanism. Our results show that chiral molecule-functionalization is able to increase the OER activity of catalysts beyond the volcano limits. A guideline for optimizing the catalytic activity via chiral molecular functionalization of hybrid 2D electrodes is given.
Factors Associated with Low IgG Response to mRNA COVID-19 Vaccine BNT162b2
Authors: Sugawa Satoshi , Nishimura Masashi , Ota Shinichiro , Morikawa Miwa , Sato Takashi , Shinoda Masahiro , Ishii Naoki , Kamachi Kenichi , Shinkai Masaharu 
Published: November 2021

Abstract
Amid pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), vaccination is hailed as one of the most effective preventive measures. An mRNA vaccine termed BNT162b2 showed satisfactory safety and efficacy in the clinical trials but several issues including variability in the individual immune response or determination of target antibody titre that exerts sufficient immune protection needs to be assessed for planning a more efficient vaccination strategy. By monitoring IgG titres before and two months after the initial administration of BNT162b2 in 655 healthcare workers by Abbott IgG II quant that detects IgG against the receptor-binding domain in S protein of SARS-CoV-2, we confirmed that hypertension, dyslipidaemia, chronic kidney disease and use of immune suppressant in addition to male gender, advanced age, and absence of previous infection were significantly associated with low antibody response to the vaccination.
Molecular Footprint of Parasite Co-introduction with Nile Tilapia in the Congo Basin
Authors: Jorissen Michiel W.P. , Vanhove Maarten P.M. , Pariselle Antoine , Snoeks Jos , Vreven Emmanuel , Šimková Andrea , Lunkayilakio Soleil Wamuini , Manda Auguste Chocha , Kasembele Gyrhaiss Kapepula , Bukinga Fidel Muterezi , Artois Tom , Huyse Tine 
Published: November 2021

Abstract
Nile tilapia, one of the most popular aquaculture species worldwide, has been introduced into the Congo Basin several times. In previous morphological studies, we showed that some of the monogenean gill parasites were co-introduced with Nile tilapia and some spilled over to native Congolese cichlids. In this study, we investigated the co-introduced monogeneans of Nile tilapia genetically from three major parts of the Congo Basin; Upper, Middle and Lower Congo. We generated sequences of Congolese native and introduced monogeneans from native and introduced tilapias and evaluate their position in a phylogeny. Additionally, we generated sequences of the same species of monogeneans co-introduced with Nile tilapia in Madagascar and of a native population of Nile tilapia from Burundi. Our results confirm the co-introductions in the Congo. We found that co-introduced parasites are less genetically diverse than native ones, and that there was no geographical pattern between introduced populations. Furthermore, our COI haplotype networks suggest multiple introduction events of Nile tilapia into the Congo Basin. Additionally, we tested the barcoding gap and the performance of mitochondrial COI and nuclear ribosomal ITS-1, 28S and 18S markers. We found a significant intra/interspecific barcoding gap of 15% for COI, but none for the other markers. Our molecular results reveal that Cichlidogyrus halli, C. papernastrema, C. tiberianus, C. cirratus and C. zambezensis are in need of taxonomic revision.
Strategies of Prosocial Behavior During the COVID-19 Pandemic
Authors: Kislyakov Pavel A. , Shmeleva Elena A. 
Published: November 2021

Background:To mitigate the potentially devastating effects of the COVID-19 pandemic, it is vital to identify psychosocial and moral resources. The care, preservation, protection, and well-being of social communities are attributes of prosocial behavior that can be such a resource.Aim:The purpose of the study is to identify the features of prosocial orientation during the COVID-19 pandemic.Methods:The sample consisted of 447 people. The study was conducted in May 2020 in the form of an online survey of subjects using Google Forms (“Portrait Values Questionnaire”).Results:The research made it possible to establish that participants were dominated by values of benevolence-universalism. During the COVID-19 pandemic, the prosocial orientation may manifest itself in the following behavioral strategies: proactive prosocial strategy of “caring for others” (true altruism, expressed in forms of volunteering, helping a stranger, and charity despite the risk of contracting a coronavirus infection); egoistic strategy of prosocial behavior “self-care through caring for others” (volunteering based on self-development; helping a stranger to improve your own psychological well-being); conventional prosocial strategy “self-care” (self-isolation and preventive behavior).Conclusion:In the long run, it is necessary to identify personal and environmental resources that can allow people to effectively implement a prosocial self-isolation strategy during the COVID-19 pandemic, as well as various forms of volunteerism.
Competencies of Educational Supervisors as a Support System to Improve Student Well-being Based Educational Program
Authors: Sulistiobudi Rezki Ashriyana , Wedyaswari Miryam , Hanami Yuliana , Cahyadi Surya 
Published: November 2021

Introduction:The well-being of students has become one of the main concerns in the Indonesian educational system nowadays. In order to apply such kind of concept in an educational program at schools, the role of an educational supervisor is critical as the supervisor performs as a facilitator as well as a controller of the program.Methods:This study investigates the competencies required by educational supervisors, especially concerning the government’s efforts to apply the concept of students’ well-being in an educational program. Since the program focuses on a particular concept, certain competencies may be needed differently from the existing educational policy. FGDs and in-depth interviews were used to analyze the influence of in-group interaction of participants in answering some questions during the discussion. These interviews were conducted with 24 educational supervisors from several cities in West Java. Thematic analysis was used to analyze the data.Results:The study identified advanced thinking, working attitudes, social skills, and managerial abilities as four main competencies, followed by 11 sub-categories that are considered important for educational supervisors to be acquired in order to support the students' well-being program.The results also highlighted that out of these four competencies, social skills are perceived as the most primary competency needed for educational supervisors, as they are agents who have to perform plentiful interactions with many parties for the implementation of the program.Conclusion:The primary competence needed for educational supervisors is social skills.
Perceived Stress of Special Education Teachers in the Context of the Person-Environment Relationship
Authors: Adigun Olufemi T. , Tijani Faizat A. , Nzima Dumisani R. , Vivekanantharasa Raveenthiran 
Published: November 2021

Background:
The teachers of learners with special needs are confronted with higher demands, enormous challenges and low reward or motivation. Hence, stress among the teachers is inevitable with a resultant effect on behavioural and learning outcomes of learners with special needs. Unfortunately, factors that contribute to perceived stress among teachers of learners with special needs, particularly in Southwest Nigeria, are yet to be established.

Objective:
To establish the relationship of some personal and environment-related factors as determinants of stress among teachers of learners with special needs.

Methods:
A total of 118 participants were randomly selected from special schools in South west Nigeria. A descriptive survey of both a quantitative and qualitative research design was adopted. Based on the person-environment fit theory, four research questions were raised and answered. A structured questionnaire and semi-structured interview were used for data collection. Quantitative data were analysed with descriptive statistics and inferential statistics at a 0.05 level of significance, while qualitative data were thematically analysed.

Results:
The study found a significant positive relationship between the student-teacher relationship and perceived stress and an inverse relationship of work experience and work motivation with perceived stress among the participants. An unfavourable working environment and lack of motivation were identified by the participants as major sources of stress.

Conclusion:
The study concluded that work environment-related factors triggered stress among teachers of learners with special needs.
Non-drug Stress Management for Patients with Cancer: A Systematic Review
Authors: Sumneangsanor Tipsuda , Ruchiwit Manyat , Weglicki Linda 
Published: November 2021

Background:
Patients with cancer suffer from the physical impacts of the disease, including pain in organs where cancer has spread to and treatment side effects. Many factors affect the mental state of cancer patients, especially stress which can cause muscle tension around the affected area and create a higher degree of pain. Stress impacts physical conditions and results in a worse quality of life. Thus, an appropriate approach to cope with, evaluate, and manage stress in cancer patients is considered crucial.

Objective:
The purpose of this systematic review was to determine and evaluate non-drug stress management guidelines for cancer patients.

Methods:
A systematic review was undertaken to synthesize knowledge concerning stress and non-drug stress management for cancer patients. Information was sourced from documents and articles published by related institutions in relevant electronic databases, including PubMed, PsycINFO, Dynamed, and ScienceDirect between 2013 and 2019. No limitations were imposed regarding the type of study design previously used. Conference abstracts were not accepted. The quality of all included studies was independently appraised by two review writers.

Results:
The search generated 129 studies, of which only 20 met the inclusion criteria. The 20 studies cover 11 studies of music for cancer prevention and 6 studies of mindfulness-based stress reduction in cancer patients. The studies utilized both quantitative and qualitative approaches, while three studies of biofeedback in cancer patients only collected quantitative data. For the outcome from the reviews, 6 studies found that music therapy, biofeedback, and mindfulness greatly impact physical alterations such as insomnia, nausea or vomiting, and pain. Furthermore, 17 studies found that non-drug management techniques had a positive impact on psychological adjustments such as stress and anxiety reduction and relaxation promotion. Non-drug stress management such as music, biofeedback, and mindfulness was found to reduce stress among patients.

Conclusion:
This review confirms that non-drug stress management approaches can reduce suffering, lead to a better quality of life, reduce mortality rates, minimize treatment costs, and prevent and mitigate unwanted symptoms in cancer patients. This approach can be adapted and applied to patients with other diseases in the future. However, because several of the reviewed studies did not have a follow-up period, the present study was unable to collect evidence suggesting how long the observed benefits will remain.