bluuebunny/crossref_metadata_2025_split huggingface dataset:
The ecological niche of reported rabies cases in Canada is similar to Alaska
Authors: Huettmann Falk , Hueffer Karsten 
Published: May 2021

AbstractThe ecology of rabies in the circumpolar North is still not well understood. We use machine learning, a geographic information system and data explicit in time and space obtained for reported rabies cases and predictors in Canada to develop an ecological niche model for the distribution of reported rabies cases in the American north (Alaska and Canada). The ecological niche model based on reported rabies cases in Canada predicted reported rabies cases in Alaska, suggesting a rather robust inference and even similar drivers on a continental scale. As found in Alaska, proximity to human infrastructure—specifically along the coast—was a strong predictor in the detection of rabies cases in Canada. Also, this finding highlights the need for a more systematic landscape sampling for rabies infection model predictions to better understand and tackle the ecology of this important zoonotic disease on a landscape scale at some distance from human infrastructure in wilderness areas.
Loss of immune homeostasis in patients with idiopathic pulmonary arterial hypertension
Authors: Heukels Peter , Corneth Odilia B J , van Uden Denise , van Hulst Jennifer A C , van den Toorn Leon M , van den Bosch Annemien E , Wijsenbeek Marlies S , Boomars Karin A , Kool Mirjam , Hendriks Rudi W 
Published: May 2021

IntroductionAutoreactivity against pulmonary vascular structures is thought to be involved in idiopathic pulmonary arterial hypertension (IPAH), but the underlying mechanisms remain poorly understood. We hypothesised that aberrant B-cell activation contributes to IPAH aetiology.MethodsMice with enhanced B-cell activation due to B-cell-specific overexpression of the B-cell receptor (BCR) signalling molecule Bruton’s tyrosine kinase (BTK) were subjected to lung injury and examined for several pulmonary hypertension (PH) indices. Peripheral blood lymphocytes from patients with IPAH (n=13), connective tissue disease-associated PAH (CTD-PAH, n=9), congenital heart disease PAH (n=7), interstitial lung disease associated PH (n=17) and healthy controls (n=19) were characterised by 14-colour flow cytometry.ResultsFollowing pulmonary injury, BTK-overexpressing mice showed prolonged activation of B cells and CXCR5+ follicular T-helper (Tfh) cells, as well as features of PH development. Patients with CTD-PAH and CHD-PAH displayed reduced proportions of circulating non-switched-memory B cells (p=0.03, p=0.02, respectively). Interestingly, we observed increased BTK protein expression in naive (p=0.007) and memory B-cell subsets of patients with IPAH and CTD-PAH. BTK was particularly high in patients with IPAH with circulating autoantibodies (p=0.045). IPAH patients had low frequencies of circulating CXCR5+ Tfh cells (p=0.005). Hereby, the increased BTK protein expression in B cells was associated with high proportions of Tfh17 (p=0.018) and Tfh17.1 (p=0.007) cells within the circulating Tfh population.ConclusionsOur study shows that pulmonary injury in combination with enhanced B-cell activation is sufficient to induce PH symptoms in mice. In parallel, immune homeostasis in patients with IPAH is compromised, as evidenced by increased BCR signalling and cTfh17 polarisation, indicating that adaptive immune activation contributes to IPAH disease induction or progression.
Predictors of poor outcomes in children with tracheoesophageal fistula/oesophageal atresia: an Australian experience
Authors: Hew Nicole Lee Chui , Grover Zubin , Paida Sanjay , Gera Sanchita , Effendy Rachel Zie Ting , Kikiros Colin , Gera Parshotam 
Published: May 2021

ObjectiveThe aim of this study is to characterize long-term morbidities of oesophageal atresia (OA) with or without tracheoesophageal fistula (TOF).MethodsInfants born with OA/TOF from 2000 to 2016 in Western Australia were included for analysis. Infants were categorized into high-risk and low-risk groups based on the presence of one or more perioperative risk factors [low birth weight, vertebraldefects, anal atresia, cardiac defects, TOF, renalanomalies, limb abnormalities (VACTERL), anastomotic leak, long gap OA, and failure to establish oral feeds within the first month] identified by a previous Canadian study. Frequency of morbidities in infants with perioperative risk factors was compared.ResultsOf 102 patients, 88 (86%) had OA with distal TOF (type C). The most common morbidities in our cohort were anastomotic oesophageal strictures (AS) (n=53, 52%), tracheomalacia (n=48, 47%), gastroesophageal reflux disease (GORD) (n=42, 41%) and recurrent respiratory tract infections (n=40, 39%). Presence of GORD (30/59 vs 12/43, p=0.04) and median frequency of AS dilatations (8 vs 3, n=59, p=0.03) were greater in the high-risk group. This study further confirmed that inability to be fed orally within the first month was associated with high morbidities.ConclusionsGastrointestinal and respiratory morbidities remain high in OA/TOF regardless of perioperative risk factors. Inability to be fed orally within the first month is a predictor of poor outcomes with high frequency of gastrointestinal and respiratory comorbidities.
Impact of wearing a surgical and cloth mask during cycle exercise
Authors: Doherty Connor J. , Mann Leah M. , Angus Sarah A. , Chan Jason S. , Molgat-Seon Yannick , Dominelli Paolo B. 
Published: May 2021

We sought to determine the impact of wearing cloth or surgical masks on the cardiopulmonary responses to moderate-intensity exercise. Twelve subjects (n = 5 females) completed three, 8-min cycling trials while breathing through a non-rebreathing valve (laboratory control), cloth, or surgical mask. Heart rate (HR), oxyhemoglobin saturation (SpO2), breathing frequency, mouth pressure, partial pressure of end-tidal carbon dioxide (PetCO2) and oxygen (PetO2), dyspnea were measured throughout exercise. A subset of n = 6 subjects completed an additional exercise bout without a mask (ecological control). There were no differences in breathing frequency, HR or SpO2 across conditions (all p > 0.05). Compared with the laboratory control (4.7 ± 0.9 cmH2O [mean ± SD]), mouth pressure swings were smaller with the surgical mask (0.9 ± 0.7; p < 0.0001), but similar with the cloth mask (3.6 ± 4.8 cmH2O; p = 0.66). Wearing a cloth mask decreased PetO2 (−3.5 ± 3.7 mm Hg) and increased PetCO2 (+2.0 ± 1.3 mm Hg) relative to the ecological control (both p < 0.05). There were no differences in end-tidal gases between mask conditions and laboratory control (both p > 0.05). Dyspnea was similar between the control conditions and the surgical mask (p > 0.05) but was greater with the cloth mask compared with laboratory (+0.9 ± 1.2) and ecological (+1.5 ± 1.3) control conditions (both p < 0.05). Wearing a mask during short-term moderate-intensity exercise may increase dyspnea but has minimal impact on the cardiopulmonary response. Novelty: Wearing surgical or cloth masks during exercise has no impact on breathing frequency, tidal volume, oxygenation, and heart rate However, there are some changes in inspired and expired gas fractions that are physiologically irrelevant. In young healthy individuals, wearing surgical or cloth masks during submaximal exercise has few physiological consequences.
Cold tolerance varies among invasive populations of the Asian clam (Corbicula fluminea)
Authors: Cvetanovska E. , Castañeda R.A. , Hendry A.P. , Conn D.B. , Ricciardi A. 
Published: May 2021

The distribution of the subtropical Asian clam (Corbicula fluminea (O.F. Müller, 1774)), one of the world’s most invasive freshwater molluscs, is reportedly constrained by a lower thermal tolerance limit of 2 °C. Although its occurrence in north temperate regions is typically restricted to artificially heated waterbodies, the species has been found to overwinter in unheated lakes and rivers. In laboratory experiments, we compared the cold tolerance of populations from several geographically distinct sites spanning 35°N to 46°N in eastern North America. Each population contained individuals that fully recovered from 2 months of continuous exposure to near-freezing (1 °C) conditions, contrary to published accounts of C. fluminea’s thermal ecology. Survivorship increased with body size and was enhanced by prior acclimation to a low temperature (10 °C) compared with a higher one (18 °C). When acclimated to 10 °C, clams from northern populations exhibited greater survivorship (55.0% ± 16.1%) than those from southern populations (26.7% ± 19.2%). However, one southern population demonstrated survivorship as great as that of the most tolerant northern population, suggesting that its clams could overwinter in unheated northern waterbodies. Differences among populations indicate either that contemporary evolution has occurred or that developmental plasticity shapes future acclimation responses.
Sequential SEM imaging of microbial calcite precipitation consolidation treatment
Authors: Scherrer Nadim C. , Kocsis Matthias , Dariz Petra , Gervais Claire 
Published: May 2021

AbstractCultural heritage built from limestone is prone to deterioration by chemical weathering, a natural process, that is enhanced by pollution. There are many historic monuments built from calcareous rocks that suffer from deterioration, and thus there have been a number of approaches over the last few decades to consolidate these types of rocks and surfaces. Using natural biological processes by fostering the activity of calcite-producing bacteria, also referred to as biomineralization, is one strategy that has also been commercialized. The base of proving the effectiveness of any surface treatment is the observation of the surface at sequential stages before and after treatment, as well as after exposure to weathering. Due to the heterogeneity of natural materials and processes, our aim was to observe identical test areas at the micron scale throughout the observation period. In order to achieve this on a tungsten SEM, we employed a beam deceleration accessory that allowed low kV imaging on non-conductive surfaces at a sufficiently high image resolution with a modified sample holder accommodating drill cores of 25 mm diameter and up to 15 mm height. The presented method is capable of producing time-sequenced images on the same test area on natural rock surface samples without manipulation for imaging purposes. This offers interesting perspectives for effective documentation of such processes in various fields.
Experimental Study of Microvascular Anastomosis: Comparison of the Conventional Method, Back Wall Technique, and Untied Stay Suture Method
Authors: Arai Satoe 
Published: May 2021

Background: Compared with the conventional microvascular anastomosis method, the back wall technique and untied stay suture method allow for better visualization of the vascular lumen and have been reported to be effective for beginner surgeons. However, there are no reports of such advantages from experimental studies. The present study compared the usefulness of the conventional method (Method C), back wall technique (Method B), and untied stay suture method (Method U) in rats. Methods: Ninety end-to-end anastomosis procedures of the right femoral artery and vein were performed in rats. The anastomosis condition was assessed at the completion of suturing one side, at the completion of vascular anastomosis, and on postoperative day 7. Results: After suturing one side, suture errors were observed in three veins with Method C. Immediately after the completion of vascular anastomosis, blood flow was impaired in one vein with Method C. On postoperative day 7, blood flow was impaired in one artery and one vein with Method C, one vein with Method B, and one artery with Method U. Conclusions: No significant differences were observed between back wall technique and the untied stay suture method. However, the conventional method was more likely to lead to suture error or impaired blood flow compared with back wall technique and the untied stay suture method.
In Vitro Comparison between the Pulvertaft Weave and the Modified Core Suture Pulvertaft Weave
Authors: Van Royen Kjell , Quintero Jorge I. , Voor Michael , Muneer Mohammed , Bouri Fadi , Muresan Claude , Tsai Tsu-Min 
Published: May 2021

Background: The Pulvertaft weave was described more than 50 years ago and is still used in tendon transfers. The aim of this study was to evaluate the strength of a modified core suture Pulvertaft weave technique and compare it to the original Pulvertaft weave traditionally used in tendon transfer surgery. Methods: 12 extensor pollicis longus tendons and extensor indices proprius tendons were harvested from fresh frozen cadavers. Six Pulvertaft weaves were performed using FiberWire 4.0 and six core suture tendon weave were performed using FiberLoop 4.0. Biomechanical analysis was performed and stifness, first failure load and ultimate failure load were measured for both set of repairs. Results: The stiffness of the core suture tendon repair (9.5 N/mm) was greater than that of the Pulvertaft repair (2.5 N/mm) The first failure load of the core suture tendon repairs (68.9 N) was greater than the Pulvertaft repairs (19.2 N) and the ultimate failure load of the core suture tendon repairs (101.8 N) was greater than the Pulvertaft repairs (21.9 N). All of these differences were statistically significant. Conclusions: The core suture Pulvertaft weave is a modification to the Pulvertaft weave used in tendon transfers. The results of this cadaveric study suggest it is 5 times stronger than the traditional Pulvertaft repair, potentially allowing it to be used with early active motion protocols after tendon transfers.
Conversion to Extension Contracture as a Means of Correction of Severe Flexion Contracture of the PIP Joint Using a Modified Dynamic External Fixator
Authors: Ohira Kenji , Hamada Yoshitaka , Toyama Takeyasu , Suga Toshimitsu , Shinko Seiko , Kohara Shoma , Saito Takanori 
Published: May 2021

Background: This is a retrospective case series investigating the outcomes using a dynamic external fixator (DEF) for treatment on severe flexion contractures at the proximal interphalangeal (PIP) joint. Severe flexion contractures of the PIP joint occurring after multiple operations and neglected over a long period of time are difficult to treat. The recurrence of contracture, even after successful treatment, is inevitable in patients with severe cases. In this study, we defined the severity of PIP joint contracture based on the active range of motion (ROM), soft tissue condition, and duration of the contracture. We also illustrated the strategy, results, and complications of using a DEF with rubber bands in these severe cases. Methods: We studied 11 fingers of 10 patients with PIP joint contracture treated by DEF. These were fixed at a small arc and neglected for an average 4.1 years (range, 1–9 years). The temporal Kirshner wire (K-wire) fixation after achieving an extension via DEF was maintained for 9.1 weeks on average. We retrospectively reviewed the results of these patients with an average 2-year follow-up. Results: Our method yielded favorable results upon retrospective evaluation. The average active ROM of the affected PIP joint improved from 90/96° to 34/83° with a functional arc and good patient satisfaction. The elastic force induced by strong rubber bands was safe and effective. The first step of joint space widening was the key to obtaining a successful joint extension afterwards. Serious progression of osteoarthritis at the PIP joint and pin-site fracture were a complication in each one case. Conclusions: In this study, we evaluate the surgical strategy of using DEFs powered by elastic torque from rubber bands to treat severe cases of flexion contractures of fingers. We first created extension contracture intentionally, followed by promoting flexion movement during follow-up in this group of patients.
The U.K.–China Climate Science to Service Partnership
Authors: Scaife Adam A. , Good Elizabeth , Sun Ying , Yan Zhongwei , Dunstone Nick , Ren Hong-Li , Li Chaofan , Lu Riyu , Wu Peili , Ke Zongjian , Ma Zhuguo , Furtado Kalli , Wu Tongwen , Zhou Tianjun , Dunbar Tyrone , Hewitt Chris , Golding Nicola , Zhang Peiqun , Allan Rob , Dale Kirstine , Lott Fraser C. , Stott Peter A. , Milton Sean , Song Lianchun , Belcher Stephen 
Published: May 2021

AbstractWe present results from the first 6 years of this major U.K. government funded project to accelerate and enhance collaborative research and development in climate science, forge a strong strategic partnership between U.K. and Chinese climate scientists, and demonstrate new climate services developed in partnership. The development of novel climate services is described in the context of new modeling and prediction capability, enhanced understanding of climate variability and change, and improved observational datasets. Selected highlights are presented from over 300 peer reviewed studies generated jointly by U.K. and Chinese scientists within this project. We illustrate new observational datasets for Asia and enhanced capability through training workshops on the attribution of climate extremes to anthropogenic forcing. Joint studies on the dynamics and predictability of climate have identified new opportunities for skillful predictions of important aspects of Chinese climate such as East Asian summer monsoon rainfall. In addition, the development of improved modeling capability has led to profound changes in model computer codes and climate model configurations, with demonstrable increases in performance. We also describe the successes and difficulties in bridging the gap between fundamental climate research and the development of novel real-time climate services. Participation of dozens of institutes through subprojects in this program, which is governed by the Met Office Hadley Centre, the China Meteorological Administration, and the Institute of Atmospheric Physics, is creating an important legacy for future collaboration in climate science and services.
Extratropical Southern Hemisphere Synchronous Pressure Variability in the Early Twentieth Century
Authors: Fogt Ryan L. , Connolly Charlotte J. 
Published: May 2021

AbstractBecause continuous meteorological observations across Antarctica did not start until the middle of the twentieth century, little is known about the full spatial pattern of pressure variability across the extratropical Southern Hemisphere (SH) in the early twentieth century, defined here as the period from 1905 to 1956. To fill this gap, this study analyzes pressure observations across the SH in conjunction with seasonal pressure reconstructions across Antarctica, which are based on observed station-to-station statistical relationships between pressure over Antarctica and the southern midlatitudes. Using this newly generated dataset, it is found that the early twentieth century is characterized by synchronous but opposite-signed pressure relationships between Antarctica and the SH midlatitudes, especially in austral summer and autumn. The synchronous pressure relationships are consistent with the southern annular mode, extending its well-known influence on SH extratropical pressure since 1957 into the early twentieth century. Apart from connections with the southern annular mode, regional and shorter-duration pressure trends are found to be associated with influences from tropical variability and potentially the zonal wavenumber 3 pattern. Although the reduced network of SH observations and Antarctic reconstruction captures the southern annular mode in the early twentieth century, reanalysis products show varying skill in reproducing trends and variability, especially over the oceans and high southern latitudes prior to 1957, which stresses the importance of continual efforts of historical data rescue in data-sparse regions to improve their quality.
Influence of Background Divergent Moisture Flux on the Frequency of North Pacific Atmospheric Rivers
Authors: HagosP Samson , LeungP L. Ruby , Garuba Oluwayemi , Patricola Christina M. 
Published: May 2021

AbstractThe frequency of North Pacific atmospheric rivers (ARs) affects water supply and flood risk over western North America. Thus, understanding factors that affect the variability of landfalling AR frequency is of scientific and societal importance. This study aims at identifying the sources of the moisture for North Pacific ARs and assessing how different modes of variability modulate these sources. To this end, the sources and variability of the background divergent component of the integrated moisture flux (DIVT) in ARs are identified using MERRA reanalysis. It is shown that in the boreal winter, this background DIVT in ARs is related to the outflow from the subsidence over the subtropics which transports moisture northward, while in summer it is related to the Asian monsoon and it transports moisture northwestward. This leads to a seasonal northwest/southeast movement of the AR frequency climatology. At the intra-seasonal scale, propagation of the Madden-Julian Oscillation introduces an anti-clockwise rotation of the background DIVT, with northward transport in phases 1 and 2, westward in 3 and 4, southward in 5 and 6 and eastward in 7 and 8, making landfall over the west coast of North America most likely during the last two phases. Similarly, El Niño Southern Oscillation variability also affects the frequency of ARs through modulation of the westerly background DIVT, favoring landfall over the US west coast during strong El Niño phases. It is shown that in general the likelihood of AR landfall over the western US is correlated with the zonal background DIVT over northeastern Pacific.
Disease severity impacts plerixafor-mobilized stem cell collection in patients with sickle cell disease
Authors: Leonard Alexis , Sharma Akshay , Uchida Naoya , Stroncek David , Panch Sandhya R. , West Kamille , Molloy Eoghan , Hughes Thomas E. , Hauffe Sara , Taylor Tiffani , Fitzhugh Courtney , Hankins Jane S. , Wilson Megan , Tsai Shengdar Q. , Weiss Mitchell J. , Hsieh Matthew , Tisdale John F. 
Published: May 2021

AbstractRecent studies suggest that plerixafor mobilization and apheresis in patients with sickle cell disease (SCD) is safe and can allow collection of sufficient CD34+ hematopoietic stem cell (HSC) collection for clinical gene therapy applications. However, the quantities of plerixafor-mobilized CD34+ cells vary between different SCD patients for unknown reasons. Twenty-three participants with SCD underwent plerixafor mobilization followed by apheresis, processing, and HSC enrichment under a phase 1 safety and efficacy study conducted at 2 institutions. Linear regression or Spearman's correlation test was used to assess the relationships between various hematologic and clinical parameters with total CD34+ cells/kg collected. Median CD34+ cells/kg after 2 or fewer mobilization and apheresis cycles was 4.0 × 106 (range, 1.5-12.0). Similar to what is observed generally, CD34+ yield correlated negatively with age (P &lt; .001) and positively with baseline (P = .003) and preapheresis blood CD34+ cells/µL (P &lt; .001), and baseline white blood cell (P = .01) and platelet counts (P = .03). Uniquely for SCD, CD34+ cell yields correlated positively with the number of days hydroxyurea was held (for up to 5 weeks, P = .01) and negatively with markers of disease severity, including hospitalization frequency within the preceding year (P = .01) and the number of medications taken for chronic pain (P = .002). Unique SCD-specific technical challenges in apheresis were also associated with reduced CD34+ cell collection efficiency and purification. Here, we describe factors that impact plerixafor mobilization success in patients with SCD, confirming known factors as described in other populations in addition to reporting previously unknown disease specific factors in patients with SCD. This trial was registered at www.clinicaltrials.gov as #NCT03226691.