bluuebunny/crossref_metadata_2025_split huggingface dataset:
Massilia Cellulosiltytica Sp. Nov., A Novel Cellulose-degrading Bacterium Isolated from Rhizosphere Soil of Rice (Oryza Sativa L.) and whole Genome Analysis
Authors: Du Chuanjiao , Li Chenxu , Cao Peng , Li Tingting , Du Dandan , Wang Xiangjing , Zhao Junwei , Xiang Wensheng 
Published: May 2021

Abstract
A bacterial strain, Gram-stain negative, rod-shaped, aerobic and cellulose-degrading, designated NEAU-DD11T, was isolated from rhizosphere soil of rice collected from Northeast Agricultural University in Harbin, Heilongjiang province, North-east China. Base on 16S rRNA gene sequence analysis, strain NEAU-DD11T belongs to the genus Massilia and shared high sequence similarities with Massilia phosphatilytica 12-OD1T (98.46 %) and Massilia putida 6NM-7T (98.41 %). Phylogenetic analysis based on the 16S rRNA gene and whole genome sequences indicated that strain NEAU-DD11T formed a stable cluster with M. phosphatilytica 12-OD1T and M. putida 6NM-7T. The major fatty acids of the strain were C16:0, C17:0-cyclo and C16:1ω7c. The respiratory quinone was Q-8. The polar lipids profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified polar lipid and an unidentified phospholipid. In addition, the digital DNA-DNA hybridization values between strain NEAU-DD11T and M. phosphatilytica 12-OD1T and M. putida 6NM-7T were 45.4% and 35.6%, respectively, which are lower than the accepted threshold value of 70%. The DNA G + C content of strain NEAU-DD11T was 66.2 %. The whole genome analysis showed the strain contained carbohydrate enzymes such as glycoside hydrolase and polysaccharide lyase, which enabled the strain to had the function of degrading cellulose. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain NEAU-DD11T represents a novel species of the genus Massilia, for which the name Massilia cellulosiltytica sp. nov. is proposed. The type strain is NEAU-DD11T (= CCTCC AB 2019141T = DSM 109721T).
Is There an Optimal Timing for Surgical Treatment of Pediatric Supracondylar Humerus Fractures in the First 24 Hours?
Authors: Okkaoglu Mustafa Caner , Ozdemir Firat Emin , Ozdemir Erdi , Karaduman Mert , Ates Ahmet , Altay Murat 
Published: May 2021

Abstract
Background:We aimed to determine the ideal surgical timing in the first 24 hours after admission to the hospital of pediatric supracondylar humerus fractures (SHF) that do not require emergent intervention. Materials and Methods:Patients who underwent surgery in our institution between January 2011 and January 2019 due to pediatric Gartland type 3 SHFs were evaluated retrospectively. Open fractures, fractures associated with vascular injury and compartment syndrome, flexion type fractures were excluded. A total of 150 Gartland type 3 were included. The effect of early (<12 hours) or late (>12 hours) surgical interventions, daytime or night-time surgeries, working or non-working hour surgeries on operative parameters (operative duration and open reduction rate, reduction quality on postoperative early radiographs) were evaluated in pediatric SHFs. Results:Early (<12 hours) or late (>12 hours), daytime or nighttime, working or non-working hour surgeries were found to be similar in Gartland type 3 patients regarding early postoperative reduction quality, duration of surgery, open reduction rate (p>0.05). Mean times passed from first admission to hospital until surgery were longer in working hour, late (>12 hours) and daytime surgery groups than non-working hour, early (<12 hours) and night-time surgery groups (p<0.001). Conclusion:Although delaying the operation to the working hours seems to prolong the time until surgery in pediatric Gartland type 3 SHF patients who do not require emergent intervention such as open fractures, neurovascular impairment and compartment syndrome, there may not be a time interval that makes a difference for the patients if surgery is performed within the first 24 hours, thus the surgery could be scheduled according to the surgeons’ preference.Level of Evidence: Level 3, Retrospective cohort study
Aggregatimonas Sangjinii Gen. Nov., Sp. Nov., A Novel Silver Nanoparticle Synthesizing Bacterium Belonging to the Family Flavobacteriaceaee
Authors: Chung Dawoon , Kim Jaoon Young Hwan , Kim Kyung Woo , Kwon Yong Min 
Published: May 2021

Abstract
A gram-negative, orange-pigmented, non-flagellated, gliding, rod-shaped, and aerobic bacterium, designated strain F202Z8T, was isolated from a rusty iron plate found in the intertidal region of Taean, South Korea. Notably, this strain synthesized silver nanoparticles (AgNPs), and 17 putative genes responsible for the synthesis of AgNPs were found in its genome. The complete genome sequence of strain F202Z8T is 4,723,614 bp, with 43.26% G + C content. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain F202Z8T forms a distinct lineage with closely related genera Maribacter, Pelagihabitans, Pseudozobellia, Zobellia, Pricia, and Costertonia belonging to the family Flavobacteriaceae. The 16S rRNA sequence similarity was < 94.5%. The digital DNA–DNA hybridization and average nucleotide identity values calculated from the whole genome-sequence comparison between strain F202Z8T and other members of the family Flavobacteriaceae were in the ranges of 12.7–16.9% and 70.3–74.4%, respectively. Growth was observed at 15–33°C (optimally at 30°C), at pH 6.5–7.5 (optimally at pH 7.0), and with the addition of 2.5–4.5% (w/v) NaCl to the media (optimally at 4.0%). The predominant cellular fatty acids were iso-C15: 0, iso-C15 :1 G, and iso-C17 :0 3-OH; the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine, five unidentified lipids, and two unidentified aminolipids. Our polyphasic taxonomic results suggested that this strain represents a novel species of a novel genus in the family Flavobacteriaceae, for which the name Aggregatimonas sangjinii gen. nov., sp. nov. is proposed. The type strain of Aggregatimonas sangjinii is F202Z8T (= KCCM 43411T = LMG 31494T).
Theoretical Study on the Noncovalent Interactions Involving Triplet Diphenylcarbene
Authors: Zhao Chunhong , Lin Hui , Shan Aiting , Guo Shaofu , Li Xiaoyan , Zhang Xueying 
Published: May 2021

Abstract
The properties of some types of noncovalent interactions formed by triplet diphenylcarbene (DPC3) have been investigated by means of density functional theory (DFT) calculations and quantum theory of atoms in molecules (QTAIM) studies. The DPC3···LA (LA = AlF3, SiF4, PF5, SF2, ClF) complexes have been analyzed from their equilibrium geometries, binding energies, charge transfer and properties of electron density. The triel bond in the DPC3···AlF3 complex exhibits a partially covalent nature, with the binding energy − 65.7kJ/mol. The tetrel bond, pnicogen bond, chalcogen bond and halogen bond in the DPC3···LA (LA = SiF4, PF5, SF2, ClF) complexes show the character of a weak closed-shell noncovalent interaction. Polarization plays an important role in the formation of the studied complexes. The strength of intermolecular interaction decreases in the order LA = AlF3 > ClF > SF2 > SiF4 > PF5. In the process of complexation, the charge transferrs from DPC3 to the antibonding orbital of AlF3/SF2/ClF, the quantity of charge transfer is very small between DPC3 and SiF4/PF5. The electron spin density transferrs from the radical DPC3 to ClF and SF2 in the formation of halogen bond and chalcogen bond, but for the DPC3···AlF3/SiF4/PF5 complexes, the transfer of electron spin density is minimal.
Serological Rapid Test for Diagnosis of SARS-COVv-2 Works Also on Gastro-intestinal Tissue Samples of COVID-19 Patients: Practical Aspects of Single Center Observational Study.
Authors: Dioscoridi Lorenzo , Massad Mutaz , Forti Edoardo , Pugliese Francesco , Cintolo Marcello , Bonato Giulia , Rosa Roberto , Mutignani Massimiliano 
Published: May 2021

Abstract
SARS-COV-2 infection spreading becomes a long and challenging medical question. The possibility to improve the diagnosis of SARS-COV-2 infection in digestive endoscopy is important because gastrointestinal symptoms can be the presenting symptoms of COVID-19.We tried to obtain a fast and “bedside” diagnosis of gastrointestinal COVID-19 by performing serological rapid test to verify the presence of SARS-COV-2 in gastrointestinal mucosal specimens.From the 4th to the 30th January 2021, regular mucosal biopsies were taken from stomach, ileum or colon of 10 COVID-19 patients (7M, 3F, mean age: 49.7 y.o.) and from 10 negative patients ( 6M, 4F, mean age: 50 y.o.) according to standard clinical indications. One specimen for each patient was placed in a sterile test-tube with 5 drops of standard reagent for rapid serological test for 10 minutes.After that period, both the specimen and the drops were put on the platform for serological rapid tests. In all COVID-19 patients, tests were positive for both IgG and IgM bands (and control bands, confirming that the test worked properly). Otherwise, the analysis of gastrointestinal specimen of negative patients showed only the control band.No differences were found for different specimens (stomach, colon or ileum). Statistical analysis conducted to compare the results of the two groups confirmed the difference between COVID-19 positive and negative groups.Applying this kind of analysis, we can immediately verify if gastrointestinal symptoms (especially diarrhea) of a non-tested patient are related to SARS-COV-2 infection and we can use this test as a rapid test in case of suspected endoscopic findings to confirm the association with SARS-COV-2 infection.In the end, the test can contribute to help in the differential diagnosis of gastrointestinal manifestations considering the long-term management of SARS-COV-2 infections.
WITHDRAWN: The Catalytic Pyrolysis Mechanism of Cellulose On ZSM-5: Based On Py-GC/MS And Density Functional Theory
Authors: Cheng Xiaoxue , Jiang Ding , Xia Zhen , Barati Bahram , Viswanathan Karthickeyan , Hu Yamin , Qian Lili , He Zhixia , Wang Shuang , Li Hongping 
Published: May 2021

Abstract
Cellulose is one of the main components of terrestrial biomass. In this study, a zeolite catalyst (ZSM-5) was used to catalyze the pyrolysis of cellulose. The components produced during pyrolysis were tested by Py-GC/MS, and the pyrolysis mechanism was analyzed by density functional theory (DFT). The results showed that furans and sugars were the primary pyrolysis products. After catalytic pyrolysis, the furfural content was significantly lower than that of non-catalytic pyrolysis. However, the yield of aromatic hydrocarbons increased significantly, especially benzene, which increased from almost zero to 13.04%. DFT further explored the specific reaction pathway of catalytic pyrolysis. It was found that under the catalysis of ZSM-5, the catalyst directly participated in most cellulose pyrolysis reactions. Also, the electrophilicity of acid sites in the reaction system played an important role. Therefore, the catalysis of the molecular sieve can significantly reduce the energy barrier of each path. In generating aromatic hydrocarbons, the decarbonylation reaction and dehydration reaction of furfural can be completely catalyzed, thereby increasing the reaction rate of generating aromatic hydrocarbons. Some deoxygenation steps occurred during the reaction, which made the catalytic pyrolysis reaction easier to develop, and improved the bio-oil quality.
Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam
Authors: Bordoloi Dibosh , Sarma Debojit , Barua Nagendra Sarma , Das Bikram Kishore 
Published: May 2021

Abstract
We carried out the morphological, biochemical, and molecular characterization of twenty cultivars of the least studied Joha (aromatic) rice indigenous to Assam. Unweighted Neighbour Joining (UNJ) clustering based on usual Euclidean distances for thirty-seven polymorphic morphological markers grouped the cultivars into three clusters with eight, eleven, and one genotype. The Joha rice cultivars showed highly significant differences for all the quantitative traits except for panicle length. The genotypic and phenotypic coefficients of variability (GCV & PCV) were high for grain yield ha-1 (24.62 & 24.85%) and filled grains panicle-1 (23.69 & 25.02%). All the traits except days to flowering and maturity, flag leaf breadth, and spikelet fertility exhibited high heritability along with high to moderate genetic advance, indicating the predominant role of additive gene action. Mahalanobis D2 analysis revealed three multi-genotypic and four mono-genotypic clusters of the cultivars. The cultivars' average polyunsaturated fatty acids were 37.9% oleic acid, 39.22% linoleic acid, and 0.5% linolenic acid. The fatty acid profile of Local Joha was superior to the other cultivars as it showed a high level of linoleic and linolenic acid and low saturated fatty acid content. Kon Joha 4 and Ronga Joha contained the highest iron (82.88 mg kg-1) and zinc (47.39 mg kg-1), respectively, while protein content of Kon Joha-1 and amylose content of Harinarayan were the highest. Joha (Bihpuria) showed the highest gel consistency of 140.50 mm. Kalijeera, Kunkuni Joha, Kon Joha-5, Manimuni Joha and Kon Joha-2 accorded a strong aroma. PCR amplified 174 alleles with a mean value of 2.64 across the 66 polymorphic SSR markers. PIC values ranged from 0.091 to 0.698, with an average of 0.326. The highly informative (PIC>0.50) markers were RM316, RM283, RM585, RM1388, RM3562, RM171, R1M30, RM118, RM11and RM29 for identification of the twenty aromatic rice cultivars. The UNJ clustering based on Jaccard's coefficients classified the cultivars into three distinct clusters with eight, ten, and two genotypes. Our study revealed the nutritional richness of these specialty Joha rice cultivars and sufficient scope for yield enhancement through their interbreeding to keep quality intact.
Simulation of Gymnastics Performance Based on MEMS Sensor
Authors: Chen Bingxin , Kuang Lifei , He Wei 
Published: May 2021

Abstract
The development and progress of multi-sensor data fusion theory and method also lay the foundation for the research of human posture tracking system based on inertial sensor. This paper mainly studies the simulation of gymnastic performance based on MEMS sensors. In the preprocessing of reducing noise interference, this paper mainly uses median filter to remove signal burr. In this paper, the use of virtual character model for gymnastics performance. The computer receives sensor data from the sink node of the motion capture device through a Bluetooth communication module. The unit calculates the quaternion output from the dynamic link library of sensor data processing, calculates the rotation and coordinate offset of the limb where each sensor node is located, and realizes the real-time rendering of the virtual human model by using the driver of the human model. At the same time, it controls the storage of sensor data, the driving of model and the display of graphical interface. When the gesture action is about to happen, a trigger signal is given to the system to mark the beginning of the action, so as to obtain the initial data of each axis signal of MEMS sensor. When the gesture action is completed, a signal to end the action is given to the system to mark the end of the action, so that the original signal data between the beginning and end of the gesture action can be captured. In order to ensure the normal communication between PS and PL, it is necessary to test the key interface. Because the data received by the SPI acquisition module is irregular, it is unable to verify whether the data is wrong. Therefore, the SPI acquisition module is replaced with an automatic incremental data module, and it is generated into an IP core to build a test platform for testing. The data show that the average measurement errors of x-axis displacement, Y-axis displacement, z-axis displacement and three-dimensional displacement are 8.17%, 7.51%, 9.72% and 8.7%, respectively. The results show that the MEMS sensor can accurately identify the action with high accuracy.
Protective Effect of Pomegranate Peels Extracts Against Stomach Peptic-Ulcer Induced By Brexin In Albino Rats
Authors: Alazzouni Ahmed S. , Daim Mohamed Abdel , Gabri Mohamed S. , Fathalla Aya S. , Albrakati Ashraf , Al-Hazani Tahani , Hassan Basma N. 
Published: May 2021

Abstract
Background: Pomegranate peel extract (PPE) is known to possess bioactive compounds such as phenolics and flavonoids, considered among the more potent antioxidants and anti-inflammatory sources.Aims: This study was designed to evaluate PPE activity's protective effect as a natural therapeutic against peptic ulcers induced by Brexin.Methods: 40 rats were divided into four groups: Control group: ten rats received normal saline treatment; Brexit group: ten rats received a single oral dose of Brexit to induce the stomach ulcer; Antodine group: ten rats received Antodine (50 mg/kg) as a commercial drug for the peptic ulcer treatment once for two weeks following a peptic ulcer; Pomegranate group: ten rats of the group received PPE (43 mg/kg) treatments. Histological, histochemical, immunohistochemical techniques were used to detect histopathological damages in stomach rats in all groups.Results: The histopathological results showed that PPE treatments following Brexin-induced peptic ulcer ameliorated histological degenerative changes in the gastric glandular. Chief and surface mucous cells that are lining gastric mucosa were regained when compared with the other groups. The histochemical results showed that PPE treatment following ulcer provided an improvement in the secretion and distribution of the polysaccharides in the epithelial cells when compared with the other groups. Also, immunohistochemical results indicated a significant decrease in immunoreactivity of cytokeratin-20, cyclooxygenase-2, and proliferating cell nuclear antigen (PCNA) in epithelial cells of rats in ulcer-model when compared with the other groups.Conclusion: PPE revealed its antiulcer activity and is recommended as a natural remedy against gastric mucosal injury induced by Brexin.
Communication Skills Education in Advance Care Planning: a Survey Study in Medical Students at the University of Antwerp
Authors: de Wiel Mick van , Bombeke Katrien , Janssens Annelies 
Published: May 2021

Abstract
Background Palliative Care (PC) medicine is strongly emerging worldwide. Despite the effort to implement PC in the basic medical curriculum in Belgium, little time is spent on it during theoretical training. For two consecutive years, a survey was presented to all students from the second master’s year medicine from the University of Antwerp. The survey consisted of demographic questions, a knowledge test about PC and an evaluation about students’ self-confidence regarding communicative skills used in end-of-life conversations. First, to evaluate students’ self-confidence regarding end-of-life conversation and mapping out possible gaps in the palliative care training at our university. Second, to assess whether our classes are able to get students to adequately reflect on possible factors that may influence end-of-life conversation.Results Students feel that not enough time is spent on palliative care education. However, good scores were obtained regarding the theoretical knowledge about PC. Students feel confident about general communicative skills, but don’t feel confident about communicative skills specific to palliative care/advance care planning.Conclusion Specific course content can help improve students’ confidence in end-of-life conversation. No difference was seen between real-life teaching or online teaching. Regularly evaluating students’ confidence can help alter course content for the future.
Identification and characterization of a non-conventional CD45 negative perivascular macrophage population within the mouse brain.
Authors: Siret Carole , Lessen Max van , Jeong Hyun-Woo , Wang Shuaiwei , Simic Milesa , de Fabritus Lauriane , Tchoghandjian Aurelie , Fallet Mathieu , Sarrazin Sandrine , Sieweke Michael , Stumm Ralf , Adams Ralf , Merker Stefan , Kiefer Friedemann , de Pavert Serge van 
Published: May 2021

Abstract
Perivascular macrophages (pvM) are closely associated with cerebral vasculature and play an essential role in drainage of the brain and regulation of the immune response. Here, using reporter mouse models and immunofluorescence on sections and whole brain, flow cytometry and single cell sequencing, we identify a Lyve1+ brain perivascular population lacking classical macrophage markers such as CD45 and Cx3cr1. We named the new non-conventional CD45 negative perivascular macrophages pvM2. These cells have a similar location, morphology and phagocytic function as conventional pvM. The pvM2 are not derived from hematopoietic stem cells, as they are negative in the VavtdT lineage tracing model. They increase in number after photothrombotic induced stroke established by flow cytometry and 3D immunofluorescence analysis. Since CD45 negative cells were typically excluded from macrophage studies, the presence of pvM2 has been previously missed and their role is of importance to assess in the brain disease models.
Ca2+ Imaging With Two-photon Microscopy to Detect the Disruption of Brain Function in Mice Administered With Neonicotinoid Insecticides
Authors: Hirai Anri , Sugio Shouta , Nimako Collins , Nakayama Shouta , Kato Keisuke , Takahashi Keisuke , Arizono Koji , Hirano Tetsushi , Hoshi Nobuhiko , Fujioka Kazutoshi , Taira Kumiko , Ishizuka Mayumi , Wake Hiroaki , IKENAKA Yoshinori 
Published: May 2021

Abstract
Neonicotinoid pesticides are insecticides that are insecticides that reportedly have untargeted effects on bees and dragonflies causing a reduction in numbers. Neonicotinoids act as neuroreceptor modulators, and some studies have reported their association with neurodevelopmental disorders. However, the effect of neonicotinoids on the central nervous system has not yet been identified. Herein, we conducted in vivo Ca2+ imaging using a two-photon microscope to detect abnormal activity of neuronal circuits in the brain using a neonicotinoid. The oral administration of acetamiprid (ACE) (20 mg/kg body weight [bw]) in mature mice with a less than the no-observed-adverse-effect level (NOAEL) and a tenth or half of the median lethal dose (LD50) of nicotine (0.33 or 1.65 mg/kg bw, respectively), as a typical nAChRs agonist, increased anxiety-like behavior associated with altered activities of the neuronal population in the somatosensory cortex. Furthermore, we detected ACE and metabolites in the brain 1 h after ACE administration. The results suggested that in vivo Ca2+ imaging using a two-photon microscope enabled the highly sensitive detection of neurotoxicant-mediated brain disturbance of nerves.