bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Helicase-tethered ORC Flip Enables Bidirectional Helicase Loading
Authors: Gupta Shalini , Friedman Larry J. , Gelles Jeff , Bell Stephen P. 
Published: October 2021

AbstractReplication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-head conformation poised to initiate bidirectional replication. This process requires ORC, Cdc6, and Cdt1. Although different Cdc6 and Cdt1 molecules load each helicase, whether two ORC proteins are required is unclear. Using colocalization single-molecule spectroscopy combined with FRET, we investigated interactions between ORC and Mcm2-7 during helicase loading. We demonstrate that a single ORC molecule can recruit both Mcm2-7/Cdt1 complexes via similar interactions that end upon Cdt1 release. Between the first and second helicase recruitment, we observe a rapid change in interactions between ORC and the first Mcm2-7. In quick succession ORC breaks the interactions mediating first Mcm2-7 recruitment, releases from its initial DNA-binding site, and forms a new interaction with the opposite face of the first Mcm2-7. This rearrangement requires release of the first Cdt1 and tethers ORC as it flips over the first Mcm2-7 to form an inverted Mcm2-7-ORC-DNA complex required for second-helicase recruitment. To ensure correct licensing, this complex is maintained until head-to-head interactions between the two helicases are formed. Our findings reconcile previous observations and reveal a highly-coordinated series of events through which a single ORC molecule can load two oppositely-oriented helicases.
Highly concentrated trehalose induces transient senescence-associated secretory phenotype in fibroblasts via CDKN1A/p21
Authors: Muto Jun , Fukuda Shinji , Watanabe Kenji , Dai Xiuju , Tsuda Teruko , Kiyoi Takeshi , Mori Hideki , Shiraishi Ken , Murakami Masamoto , Higashiyama Shigeki , Mizukami Yoichi , Sayama Koji 
Published: October 2021

AbstractTrehalose is the nonreducing disaccharide of glucose, evolutionarily conserved in invertebrates, but does not exist in vertebrates. The living skin equivalent (LSE) is an organotypic coculture containing keratinocytes cultivated on fibroblast-populated dermal substitutes. We demonstrated that human primary fibroblasts treated with highly concentrated trehalose promote significantly extensive spread of the epidermal layer of LSE without any deleterious effects. The RNA-seq analysis data and Ingenuity pathway analysis of the differentially expressed genes of trehalose-treated 2D and 3D fibroblasts at early time points revealed the involvement of the CDKN1A pathway, which is necessary for the marked upregulation of growth factors including DPT. By contrast, the mRNA-seq data of LSEs 2-weeks after air exposure indicated that gene expression profiles are similar for untreated and trehalose-treated cells in both keratinocytes and fibroblasts. The trehalose-treated fibroblasts were positive for senescence-associated β-galactosidase with the significantly downregulated expressions of LMNB1. Finally, we demonstrated that transplantation of the dermal substitute with trehalose-treated fibroblasts accelerated wound closure and increased capillary formation significantly in the experimental mouse woundsin vivo. These data indicate that high-concentration trehalose can induce the beneficial senescence-associated secretory phenotype in fibroblasts via CDKN1A/p21, which may be therapeutically useful for optimal wound repair.
Transcript accumulation rates in the earlyCaenorhabditis elegansembryo
Authors: Sivaramakrishnan Priya , Watkins Cameron , Murray John Isaac 
Published: October 2021

ABSTRACTDynamic changes in transcription are widespread in developing embryos, where cell cycles are rapid and cell fate decisions need to be made quickly, often before the next cell division. Some fate decisions in the earlyCaenorhabditis elegansembryo overcome these constraints through the rapid production of high absolute levels of transcription factor mRNAs. Single cell accumulation rates for a small subset of developmental genes are known, but genome-scale measurements are lacking. Furthermore, how different aspects of transcription kinetics are fine-tuned for different genes to achieve the appropriate RNA levels is still being worked out. We describe a novel strategy to analyze single cell RNA sequencing data from the earlyC. elegansembryo. We estimate the absolute accumulation rates of zygotic genes up to the 16-cell stage and calibrate predicted rates with single molecule transcript imaging. We show that rapid transcript accumulation is common across different cell types and lineages and rates are the highest soon after zygotic transcription begins. High-rate transcription is a characteristic of genes encoding transcription factors with functions in cell fate specification. These genes share common genomic features and are more likely to have undergone recent duplication. We identify core promoter motifs that might drive high absolute RNA accumulation rates. We measured the contributions of core promoter elements to accumulation rate for one rapidly accumulating gene,ceh-51, which is required for mesoderm development. We find that mutating individual motifs modestly decreases the accumulation rate ofceh-51mRNA, suggesting multifactorial control of transcript accumulation rates. These results are a step towards estimating absolute transcription kinetics during embryonic fate specification and understanding how transcript dosage drives developmental decisions.
Value and growth stock returns: international evidence (JES)
Authors: Neves Maria Elisabete , Abreu Pinto Mário , Assunção Fernandes Carla Manuela de , Simões Vieira Elisabete Fátima 
Published: October 2021

Purpose
This study aims to analyze the returns obtained from companies with strong growth potential (growth stocks) and the returns from companies with quite low stock prices, but with high value (value stocks).

Design/methodology/approach
The sample comprises monthly data, from January 2002 to December 2016, from seven countries, Germany, France, Switzerland, the UK, Portugal, the USA and Japan. The authors have used linear regression models for three different periods, the pre-crisis, subprime crisis and post-crisis period.

Findings
The results point out that the performance of value and growth stocks differs from different periods surrounding the global financial crisis. In fact, for six countries, value stocks outperformed growth stocks in the period that precedes the subprime crisis and during the crisis, this tendency remained only for France, Portugal and Japan. This trend changed in the period following the crisis. The results also show that investor sentiment has a robust significance in value and growth stock returns, mostly in the period before the crisis, highlighting that the investor sentiment is more significant in the moments that the value stocks outperformed.

Originality/value
As far as the authors know, this is the first work that, taking into account the future research lines of Capaul et al. (1993), investigates whether the results obtained by those authors remain current, meeting the authors’ challenge and covering the gap of recent studies on the performance of value and growth stocks. Besides, the authors have introduced a new country, heavily punished by both the global financial crisis and the sovereign debt crisis to understand whether there are significant differences in investment styles and whether this is related to the different economies. Also, in this context, the authors were pioneers in adding investor sentiment as an exogenous variable in the influence of stock returns.
Why travelers switch to the sharing accommodation platforms? A push-pull-mooring framework
Authors: Chi Maomao , Wang Junjing , Luo Xin (Robert) , Li Han 
Published: October 2021

Purpose
Drawing on and extending the push-pull-mooring (PPM) framework, this paper aims to empirically explore the influencing mechanism of traveler switching intention from the hotel reservation platforms to the sharing accommodation platforms (SAPs).

Design/methodology/approach
This study adopts structural equation modeling to analyze the 543 responses collected among hotel reservation platforms and SAPs travelers.

Findings
The results support the positive effect of both push factors (e.g. dissatisfaction with product, service and information quality of hotels on the hotel reservation platform) and pull factors (e.g. price value, authenticity, interaction, home benefits and novelty of SAPs) on traveler switching intention. Except for the negative effect of switching cost, other mooring factors including prior switching experience and social influence positively affect traveler switching intention. The authors also found the switching cost negatively and prior switching experience positively moderated the push effects on traveler switching intention, while the social influence positively moderated the pull effects on traveler switching intention.

Research limitations/implications
Recommendations of future SAP participation research to consider the competing platforms, the unique experiences of SAPs and mooring factors. Examining the factors of different sources is also useful for practitioners to better understand travelers’ demands and to improve the overall welfare of travelers.

Originality/value
This paper embraces an extended PPM framework to explore traveler switching intention in online travel platforms. Moreover, this paper provides unique insights into the switching behavior from the hotel reservation platforms to the SAPs.
Reducing the filter bubble effect on Twitter by considering communities for recommendations
Authors: Grossetti Quentin , du Mouza Cedric , Travers Nicolas , Constantin Camelia 
Published: October 2021

Purpose
Social network platforms are considered today as a major communication mean. Their success leads to an unprecedented growth of user-generated content; therefore, finding interesting content for a given user has become a major issue. Recommender systems allow these platforms to personalize individual experience and increase user engagement by filtering messages according to user interest and/or neighborhood. Recent research results show, however, that this content personalization might increase the echo chamber effect and create filter bubbles that restrain the diversity of opinions regarding the recommended content.

Design/methodology/approach
The purpose of this paper is to present a thorough study of communities on a large Twitter data set that quantifies the effect of recommender systems on users’ behavior by creating filter bubbles. The authors further propose their community-aware model (CAM) that counters the impact of different recommender systems on information consumption.

Findings
The authors propose their CAM that counters the impact of different recommender systems on information consumption. The study results show that filter bubbles effects concern up to 10% of users and the proposed model based on the similarities between communities enhance recommendations.

Originality/value
The authors proposed the CAM approach, which relies on similarities between communities to re-rank lists of recommendations to weaken the filter bubble effect for these users.
Impact of room service on nutritional intake, plate and production waste, meal quality and patient satisfaction and meal costs: A single site pre‐post evaluation
Authors: Neaves Bianca , Bell Jack J. , McCray Sally 
Published: October 2021

AbstractAimRoom service is a patient‐focused foodservice model gaining interest in Australian hospitals following demonstrated patient and organisational benefits. This study aimed to compare nutritional intake, waste, patient satisfaction, meal costs and meal quality between a bought‐in, thaw‐retherm foodservice model and a cook‐fresh, on‐demand room service model at a large tertiary public hospital.MethodsA retrospective analysis of quality assurance data compared thaw‐retherm to room service. Nutritional intake and plate waste were measured using a visual intake analysis tool; production waste was measured using weighted analysis methodology; patient satisfaction was measured using a validated patient satisfaction survey; meal quality was assessed using a validated meal quality audit tool, and meal costs were obtained from hospital finance reports. Independent sample t‐tests or nonparametric equivalent (Mann‐Whitney U‐test) for continuous variables and Pearson's Chi‐square for categorical data were applied for comparative purposes.ResultsAverage energy and protein intake, as well as percentage requirements met, improved between thaw‐retherm and room service (4320 kJ/day vs 7265 kJ/day; 42.4 g/day vs 82.5 g/day; and 46% vs 80.7%; 49.9% vs 98.4%; all P < .001. Reductions in plate waste (40% vs 15%) and production waste (15% vs 5.6%, P < .001) were observed and food costs decreased by 9% with room service. Meal quality audit results improved, and patient satisfaction increased with % respondents satisfied increasing from 75.0% to 89.8% (χ2 9.985[2]; P = .007) for room service.ConclusionsThis research demonstrates significant improvements in patient and organisational outcomes with room service compared to a thaw‐retherm model in a large public hospital.
On the need for rigorous welfare and methodological reporting for the live capture of large carnivores: A response to de Araujo et al. (2021)
Authors: Caravaggi Anthony , Amado Talita F. , Brook Ryan K. , Ciuti Simone , Darimont Chris T. , Drouilly Marine , English Holly M. , Field Kate A. , Iossa Graziella , Martin Jessica E. , McElligott Alan G. , Mohammadi Alireza , Nayeri Danial , O’Neill Helen M. K. , Paquet Paul C. , Périquet Stéphanie , Proulx Gilbert , Rabaiotti Daniella , Recio Mariano R. , Soulsbury Carl D. , Tadich Tamara , Wynn‐Grant Rae 
Published: October 2021

Abstract

De Araujo et al. (Methods in Ecology and Evolution, 2021, https://doi.org/10.1111/2041‐210X.13516) described the development and application of a wire foot snare trap for the capture of jaguars Panthera onca and cougars Puma concolor. Snares are a commonly used and effective means of studying large carnivores. However, the article presented insufficient information to replicate the work and inadequate consideration and description of animal welfare considerations, thereby risking the perpetuation of poor standards of reporting.

Appropriate animal welfare assessments are essential in studies that collect data from animals, especially those that use invasive techniques, and are key in assisting researchers to choose the most appropriate capture method. It is critical that authors detail all possible associated harms and benefits to support thorough review, including equipment composition, intervention processes, general body assessments, injuries (i.e. cause, type, severity) and post‐release behaviour. We offer a detailed discussion of these shortcomings.

We also discuss broader but highly relevant issues, including the capture of non‐target animals and the omission of key methodological details. The level of detail provided by authors should allow the method to be properly assessed and replicated, including those that improve trap selectivity and minimize or eliminate the capture of non‐target animals.

Finally, we discuss the central role that journals must play in ensuring that published research conforms to ethical, animal welfare and reporting standards. Scientific studies are subject to ever‐increasing scrutiny by peers and the public, making it more important than ever that standards are upheld and reviewed.

We conclude that the proposal of a new or refined method must be supported by substantial contextual discussion, a robust rationale and analyses and comprehensive documentation.
Identification of distinct and age‐dependent p16High microglia subtypes
Authors: Talma Nynke , Gerrits Emma , Wang Boshi , Eggen Bart J.L. , Demaria Marco 
Published: October 2021

AbstractCells expressing high levels of the cyclin‐dependent kinase (CDK)4/6 inhibitor p16 (p16High) accumulate in aging tissues and promote multiple age‐related pathologies, including neurodegeneration. Here, we show that the number of p16High cells is significantly increased in the central nervous system (CNS) of 2‐year‐old mice. Bulk RNAseq indicated that genes expressed by p16High cells were associated with inflammation and phagocytosis. Single‐cell RNAseq of brain cells indicated p16High cells were primarily microglia, and their accumulation was confirmed in brains of aged humans. Interestingly, we identified two distinct subpopulations of p16High microglia in the mouse brain, with one being age‐associated and one present in young animals. Both p16High clusters significantly differed from previously described disease‐associated microglia and expressed only a partial senescence signature. Taken together, our study provides evidence for the existence of two p16‐expressing microglia populations, one accumulating with age and another already present in youth that could positively and negatively contribute to brain homeostasis, function, and disease.
Let complete numbers speak for themselves
Authors: Fiorentino Renée , Jefferson Karen , Lichtman Ronnie 
Published: October 2021

AbstractThe authors describe the challenges they encountered, having attempted to retrospectively complete a home birth outcome data set for New York State. In addition, they provide a compelling argument for a midwifery data collective that would bring together health record data for all midwife‐attended births nationwide, regardless of setting.
A component Markov regime‐switching autoregressive conditional range model
Authors: Harris Richard D. F. , Mazibas Murat 
Published: October 2021

AbstractIn this article, we develop one‐ and two‐component Markov regime‐switching conditional volatility models based on the intraday range and evaluate their performance in forecasting the daily volatility of the S&P 500 Index. We compare the performance of the models with that of several well‐established return‐ and range‐based volatility models, namely EWMA, GARCH, and FIGARCH models, the Markov regime‐switching GARCH model, the hybrid EWMA model, and the CARR model. We evaluate the in‐sample goodness of fit and out‐of‐sample forecast performance of the models using a comprehensive set of statistical and economic loss functions. To assess the statistical performance of the models, we use mean error metrics, directional predictive ability tests, forecast evaluation regressions, and pairwise and joint tests; and to appraise the economic performance of the models, we use value at risk coverage tests and risk management loss functions. We show that the proposed range‐based Markov switching conditional volatility models produce more accurate out‐of‐sample forecasts, contain more information about true volatility, and exhibit similar or better performance when used for the estimation of value at risk. Our results are robust to the choice of volatility proxy, estimation sample size, out‐of‐sample evaluation period, and alternative error distributions.
G protein pathway suppressor 2 suppresses gastric cancer by destabilizing epidermal growth factor receptor
Authors: Si Yuan , Zhang Haitao , Peng Peng , Zhu Chu , Shen Jie , Xiong Yilian , Liu Xuewen , Xiang Yuchen , Li Wenjuan , Ren Yuliang , Wan Fang , Zhang Liang , Liu Ying 
Published: October 2021

AbstractG protein pathway suppressor 2 (GPS2) is expressed in most human tissues, including the stomach. However, the biological functions of GPS2 in cancer, as well as the underlying molecular mechanisms, remain poorly understood. Here, we report that GPS2 expression was aberrantly downregulated in gastric cancer (GC) tissues compared with control tissues. Clinicopathologic analysis showed that low GPS2 expression was significantly correlated with pathological grade, lymph node stage, and invasive depth. Kaplan‐Meier analysis indicated that patients with low GPS2 expression showed poorer overall survival rates than those with high GPS2 expression. Moreover, GPS2 overexpression decreased GC cell proliferation, colony formation, tumorigenesis, and invasion. Overexpression of GPS2 reduced the protein expression of epidermal growth factor receptor (EGFR) and inhibited its downstream signaling in GC cells. Interestingly, GPS2 decreased EGFR protein expression, which was reversed by a lysosome inhibitor. Furthermore, GPS2 reduced EGFR protein stability by enhancing the binding of EGFR and an E3 ligase, c‐Cbl, which promoted the ubiquitination of EGFR, ultimately leading to its degradation through the lysosomal pathway. Further analysis indicated that GPS2 activated autophagy and promoted the autophagic flux by destabilizing EGFR. Taken together, these results suggest that low GPS2 expression is associated with GC progression and provide insights into the applicability of the GPS2‐EGFR axis as a potential therapeutic target in GC.
Trends in climatically driven extreme growth reductions of Picea abies and Pinus sylvestris in Central Europe
Authors: Treml Václav , Mašek Jiří , Tumajer Jan , Rydval Miloš , Čada Vojtěch , Ledvinka Ondřej , Svoboda Miroslav 
Published: October 2021

AbstractExtreme tree growth reductions represent events of abrupt forest productivity decline and carbon sequestration reduction. An increase in their magnitude can represent an early warning signal of impending tree mortality. Yet the long‐term trends in extreme growth reductions remain largely unknown. We analyzed the trends in the proportion of trees exhibiting extreme growth reductions in two Central‐European conifer species—Pinus sylvestris (PISY) and Picea abies (PCAB)—between 1901 and 2018. We used a novel approach for extreme growth reduction quantification by relating their size to their mean recurrence interval. Twenty‐eight sites throughout Czechia and Slovakia with 1120 ring width series representing high‐ and low‐elevation forests were inspected for extreme growth reductions with recurrence intervals of 15 and 50 years along with their link to climatic drivers. Our results show the greatest growth reductions at low‐elevation PCAB sites, indicating high vulnerability of PCAB to drought. The proportions of trees exhibiting extreme growth reductions increased over time at low‐elevation PCAB, decreased recently following an abrupt increase in the 1970–1980s at high‐elevation PCAB, and showed nonsignificant trends in high‐ and low‐elevation PISY. Climatic drivers of extreme growth reductions, however, shifted over time for all site categories as the proportion of low‐temperature‐induced extreme growth reductions declined since the 1990s, whereas events caused by drought consistently increased in frequency during the same period. We observed higher growth volatility at the lower range of distribution compared with the upper range margin of PISY and PCAB. This will undoubtedly considerably impact tree growth and vitality as temperatures and incidence of drought in Central Europe are expected to further increase with ongoing climate change.
No apparent p53 activation in CRISPR‐engineered gene‐edited rabbits
Authors: Zhang Tao , Li JinZe , Wang Tian , Zhao Feiyu , Sui Tingting 
Published: October 2021

AbstractClustered regularly interspaced short palindromic repeats‐CRISPR‐associated 9 (CRISPR‐Cas9) and base editors (BEs) are revolutionary gene‐editing technology that has been widely utilized in biology, biotechnology and medicine. However, recent reports show that CRISPR‐Cas9‐mediated genome editing can induce a p53‐mediated stress response and cell cycle arrest in human cells, while not illustrated in gene‐editing animals. In the study, to verify whether there is a phenomenon of p53 activation, by analysing nine gene‐edited rabbits using CRISPR‐Cas9 and BEs, we provide the first evidence that no apparent p53 expression changes in those rabbits generated by Cas9 or BE‐edited, suggesting that p53 may not need to consider for application in gene‐edited animals.