bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of homeobox 5 predicts poor prognosis: A potential therapeutic target for glioma
Authors: Xu Chengran , Huang Jinhai , Yang Yi , Li Lun , Li Guangyu 
Published: April 2021

Abstract
Background: The homeobox gene 5 (HOXB5) encodes a transcription factor that regulates the central nervous system embryonic development. Of note, its expression pattern and prognostic role in glioma remain unelucidated. This study aimed to identify the relationship between HOXB5 and glioma by investigating the HOXB5 expression data from the The Cancer Genome Atlas (TCGA) and The Genotype Tissue Expression (GTEx) databases and validating the obtained data using the Chinese Glioma Genome Atlas (CGGA) database. Kaplan-Meier and univariate cox regression analyses were performed to assess the prognostic value of HOXB5. The key functions and signaling pathways of HOXB5 were analyzed using GSEA and GSVA. Immune infiltration was calculated using Microenvironment Cell Populations-counter (MCP-counter), single-sample Gene Set Enrichment Analysis (ssGSEA), and ESTIMATE algorithms.Result: HOXB5 expression was elevated in glioma tissues. The increased levels of HOXB5 were significantly correlated with a higher WHO grade and aggressive cancer phenotypes. HOXB5 overexpression represented a risk factor that was associated with shorter overall survival (OS) while exhibiting a moderate forecast efficiency in most clinical subgroups. These results were validated using the CGGA and Rembrandt datasets. Furthermore, the functional analysis showed enrichment of angiogenesis, the IL6/JAK-STAT3 pathway, and inflammatory response in the tissues that showed high expression of HOXB5. Lastly, the high expression of HOXB5 was associated with enrichment of Tregs and MDSCs, and HOXB5 expression was shown to play a role in several immune checkpoint genes.Conclusions: HOXB5 may serve as a predictive factor of glioma malignancy and prognostic status and represents potential as a molecular treatment candidate.
Activation of adenosine receptor A2a by gut symbionts promotes immune tolerance
Authors: Kostic Aleksandar , Xu Tao , Zhao Lidan , Islam Md Zohorul , Pham Loc-Duyen , Corsi Martina , Phillips Gregory , Wannemuehler Michael , Benoist Christophe 
Published: April 2021

Abstract
The gut microbiota is critical to immune homeostasis, but our understanding of the underlying molecular mechanisms is very limited. Here, we demonstrate a division of labor among members of the eight-membered “model microbiome” altered Schaedler flora in promoting distinct immunophenotypes. We report that Parabacteroides goldsteinii ASF519 induces immune tolerance by promoting interleukin (IL)-10 production in a variety of myeloid-derived immune cells. The IL-10 induction is dependent on the activation of adenosine receptor A2a by microbial enzymes of the methionine cycle. ASF519 colonization in mice increased the level of adenosine in ceca and induced IL-10 secreting dendritic cells in colonic lamina propria. These immunophenotypes were pharmacologically reversed by A2a blockage. In mouse models of human autoimmune diseases, ASF519 supplementation significantly ameliorated insulitis in type 1 diabetes and collagen-induced arthritis. This study unveils a novel paradigm of gut microbiota-adenosine receptor interactions in immune tolerance and potentially provides a new therapeutic strategy for immune disorders.
Prospective Observational Study of Factors Related to the Movement Subscale Score of the Japanese Orthopaedic Association Hip-Disease Evaluation Questionnaire Six Months After Total Hip Arthroplasty
Authors: Sekita Junya , Takahira Naonobu , Iwamura Genki , Kusaba Atsushi , Kondo Saiji 
Published: April 2021

Abstract
Background

The movement subscale score of the Japanese Orthopaedic Association Hip-Disease Evaluation Questionnaire (JHEQ) is reportedly lower than other subscale scores after total hip arthroplasty (THA). This study aimed to assess factors related to the movement subscale score of the JHEQ six months after THA.
Methods

Participants were 90 female patients with hip osteoarthritis who underwent THA. We examined clinical background factors and surgical information, and measured hip joint range of motion, lower extremity muscle strength, maximum walking speed, physical activity, Japanese Orthopaedic Association score, and JHEQ score. We also measured the number of steps and “fast walking time” as physical activity, and calculated the moderate intensity activity ratio. A stepwise logistic regression analysis was used to identify factors related to high and low movement subscale scores.
Results

Six months after surgery, the total score and all subscale scores of the JHEQ were significantly higher compared to preoperative scores (p < 0.01). The movement subscale score was lower compared to other subscale scores (p < 0.01). The stepwise multiple regression analysis revealed that preoperative movement subscale score (odds ratio: 1.26, 95%CI: 1.08–1.47, p = 0.003), activities of daily living category of the Japanese Orthopaedic Association hip score (odds ratio: 1.66, 95%CI: 1.18–2.33, p = 0.004), and moderate intensity activity ratio (odds ratio: 1.05, 95%CI: 1.00-1.11, p = 0.035) are factors that determine high and low movement subscale scores.
Conclusion

The postoperative movement subscale score is related to not only the ability to conduct activities of daily living and preoperative movement subscale score, but also the moderate intensity activity ratio.
SARS-CoV2, A Threat to Marine Mammals? A Study From Italian Seawaters
Authors: Audino Tania , Grattarola Carla , Centelleghe° Cinzia , Peletto Simone , Giorda Federica , Florio Caterina Lucia , Caramelli Maria , Bozzetta Maria Elena , Mazzariol Sandro , Guardo Giovanni Di , Lauriano Giancarlo , Casalone Cristina 
Published: April 2021

Abstract
Zoonotically transmitted coronaviruses were responsible for three disease outbreaks since 2002, with the “Severe Acute Respiratory Syndrome Coronavirus-2” (SARS-CoV-2) causing the dramatic “Coronavirus Disease-2019” (CoViD-19) pandemic, which affected public health, economy, and society on a global scale. The impacts of the SARS-CoV-2 pandemic permeate into our environment and wildlife as well; in particular, concern has been raised about the viral occurrence and persistence in aquatic and marine ecosystems. The discharge of untreated wastewaters carrying infectious SARS-CoV-2 into natural water systems that are home of sea mammals may have dramatic consequences on vulnerable species. The efficient transmission of coronaviruses raises questions regarding the contributions of virus-receptors interactions. The main receptor of SARS-CoV-2 is Angiotensin Converting Enzyme-2 (ACE-2), serving as a functional receptor for the viral spike (S) protein. This study was aimed, through the comparative analysis of the ACE-2 receptor with the human one, at assessing the susceptibility to SARS-CoV-2 of the different species of marine mammals living in Italian waters. We also determined, by means of immunohistochemistry, ACE-2 receptor localization in the lung tissue from different cetacean species, in order to provide a preliminary characterization of ACE-.2 expression in the marine mammals’ respiratory tract. Furthermore, in order to evaluate if and how wastewater management in Italy may lead to susceptible marine mammal populations being exposed to the virus, geo-mapping data of wastewater plants, associated to the identification of specific stretches of coast more exposed to extreme weather events, overlapped to marine mammal population data, were carried out. Results showed the SARS-CoV-2 exposure for marine mammals inhabiting Italian coastal waters. Thus, we highlight the potential hazard of reverse zoonotic transmission of SARS-CoV-2 infection, along with its impact on marine mammals regularly inhabiting the Mediterranean Sea, whilst also stressing the need of appropriate action to prevent further damage to specific vulnerable populations.
Superiority of Mild Interventions Against COVID-19 on Public Health and Economic Measures
Authors: Niwa Makoto , Hara Yasushi , Matsuo Yusuke , Narita Hodaka , Lim Yeongjoo , Sengoku Shintaro , KODAMA KOTA 
Published: April 2021

Abstract

During the global spread of COVID-19, Japan has been among the top countries to maintain a relatively low number of infections, despite implementing limited institutional interventions. Using a Tokyo Metropolitan dataset, this study investigated how these limited intervention policies have affected public health and economic conditions in the COVID-19 context. A causal loop analysis suggested that there were risks to prematurely terminating such interventions. On the basis of this result and subsequent quantitative modelling, we found that the short-term effectiveness of a short-term pre-emptive stay-at-home request caused a resurgence in the number of positive cases, whereas an additional request provided a limited negative add-on effect for economic measures (e.g. the number of electronic word-of-mouth (eWOM) communications and restaurant visits). These findings suggest the superiority of a mild and continuous intervention as a long-term countermeasure under epidemic pressures when compared to strong intermittent interventions.
The first principle calculation of improving p-type characteristics of BXAl1-xN
Authors: Lu Zhengqian , Wang Fang , Liu Yuhuai 
Published: April 2021

Abstract
AlN is one of the third-generation semiconductor materials with wide application prospects due to its 6.2eV band gap. In the application of semiconductor deep ultraviolet lasers, progress is slow due to the difficulty in obtaining p-type AlN with good performance. In this paper, the commonly used Mg dopants are abandoned, and the research on BAlN alloys is replaced. The improvement of the p-type properties of AlN crystals by B composition is studied by first-principles calculations. The results show that the addition of B composition can significantly inhibit the intrinsic n-type performance of AlN, which is beneficial to the formation of p-type AlN. At the same time, it has been found that when the composition of B reaches 19.5%, the BAlN compound semiconductor changes from n-type characteristics to p-type characteristics. PACS: 61.72.uj, 71.55.Eq, 73.61.Ey
Endoscopic Submucosal Dissection of Pseudo-polyps in the Right Colon Using a Flexible Grasper With Articulating Distal Tip and Elbow-Like Bending
Authors: Urakawa Shinya , Hirashita Teijiro , Hirashita Yuka , Lowenfeld Lea , Gurram Krishna , Nishimura Makoto , Milsom Jeffrey 
Published: April 2021

Abstract
Endoscopic submucosal dissection (ESD) is challenging in the right colon. Traction devices can make it technically easier. In this study, we evaluated a flexible grasper with articulating tip and elbow-like bending (IgE) through a double-balloon surgical platform (DESP), compared with an earlier generation grasper without elbow-like bending (Ig). The reach of Ig/IgE was investigated at eight locations using a synthetic colon within a 3D model. Using a fresh porcine colorectum, 4 cm pseudo-polyps were created at the posterior wall of the ascending colon. Fifty-four ESD procedures were performed using three techniques: standard ESD (STD), ESD using Ig (DESP+Ig), and ESD using IgE (DESP+IgE). IgE was able to reach the full circumference at all the locations, whereas the medial walls proximal to the descending colon were out of Ig’s reach. Compared with the STD, both DESP+Ig and DESP+IgE showed significantly shorter procedure time (STD vs. DESP+Ig vs. DESP+IgE = median 48.9 min vs. 38.6 vs. 29.9) and fewer injuries (1.5 vs. 0 vs. 0). Moreover, the DESP+IgE had a shorter procedure time than the DESP+Ig (p=0.0025). The IgE with DESP increased instrument reach compared to Ig, and likely represented a traction tool for excision of large pseudo-polyps in the right colon.
Rapid Exome Sequencing for Critically Ill Children: Implementation and Challenges in the Asian Context
Authors: Fong Nikki , Lim Jiin Ying , Cham Breana , Kam Sylvia , Goh Chew Yin , Wei Heming , Tan Yuen Ming , Ee Hui Jing , Ching Leng Kee , Low Wendy , Kuan Jyn Ling , Law Hai Yang , Kong Juin Yee , Loh Lik Eng , Koh Ai Ling , Ting Teck Wah , Lai Angeline , Ng Ivy , Lim Weng Khong , Tan Ee Shien , Tan Ene Choo , Jamuar Saumya Shekhar 
Published: April 2021

Abstract
Objective: Use rapid next-generation sequencing (NGS) to improve our diagnostic yield in critically ill paediatric patients with suspected genetic disorders in the Asian setting.Design: A diagnostic study conducted between April 2018 and January 2019.Methods: Next-generation sequencing was performed with the TruSight One gene panel (targeting 4813 genes) followed by MiSeq sequencing on 10 patients who presented with suspected genetic disorders as assessed by their attending physicians. Results: In 4 of the 10 cases (40%), a genetic diagnosis was achieved, with one further case diagnosed on re-analysis of data 2 years later. The median turn-around time (TAT) for results was 9.5 working days (range 5-19 days). Challenges faced during implementation included sample availability, managing parental and primary physician expectations, cost of testing, and bioinformatic resources.Conclusion: RapidSeq is an effective method for diagnosing patients with rare diseases, which aids in shortening the diagnostic odyssey, while allowing clinicians to appropriately tailor management for the underlying disorder, and provide accurate genetic counselling for families. However, challenges such as cost and insurance implications still remain a barrier to more widespread use of genomic testing in the local setting, and continued efforts will be required to optimise RapidSeq for use in paediatric patients in the ICU.
Lesion Scores, Oocysts Output, Hematological and Histopathological Changes of the 7 Days Life Cycle of Eimeria Tenella in Broilers.
Authors: Nonkookhetkhong Tippayaporn , Chalalai Thanyakorn 
Published: April 2021

Abstract
Eimeria tenella is a protozoa which is a causative agent of avian cecal coccidiosis. The disease results in bleeding, diarrhea, weight losses, high morbidity and mortality in chickens. This study investigated lesion scores, oocysts output, hematological and histopathological changes of the 7 days life cycle of E. tenella. Fifty-six, broilers were randomly divided into 2 groups of which group 1 were uninfected and group 2 were infected with 2x104 E. tenella oocysts. Lesion scores and oocyst output of the infected group were significantly higher than in the uninfected group at 5, 6 and 7 dpi (p < 0.05). PCV was significantly lower than in the uninfected group at 3 to 7 dpi (p < 0.05). WBC count was significantly higher than in the uninfected group at 4 dpi (p < 0.05). Lymphocytes and heterophils were significantly higher than in the uninfected group at 4 dpi (p < 0.05). Monocytes were significantly higher than in the uninfected group from 3 to 5 dpi (p < 0.05). The developmental stages of E. tenella in cecum tissue during the 7 days life cycle included trophozoite at 2 dpi, first generation of schizonts at 3 dpi, secondary generation of merozoites and schizonts at 4 and 5 dpi and gametocytes and oocysts at 6 and 7 dpi. These finding suggest that monocytes were the most effective white blood cell on E. tenella infection and secondary generation schizonts was the most pathogenic developmental stage of E. tenella.
Ruthenium(III) Based Diimine Complexes; Synthesis, Characterization, PXRD Study and Catalytic Hydrogenation of Cyclohexene
Authors: Ramadan Abd El-Motaleb M , Ibrahim Mohamed M. , Mersal Gaber A. M. , Fathy Ahmed M. , El-Hendawy Morad M. , Al-Harbi Sami A. , Al-Saidi Hamed M. 
Published: April 2021

Abstract
This study deals with preparation and characterization of a group of RuIII-chelates contains tetradentate diimine ligands. These quadridentate ligands are derived from 2-OH-1-naphthaldehyde and a number of aliphatic diamines where the number of methylene groups between the two azomethine nitrogen donors varied from two to six are the components of quadridentate ligands. The pure isolated compounds were subjected to several physicochemical investigations to assign their structures. Spectral and magnetic measurements suggested a distorted octahedral arrangement of the six coordinate diimine ruthenium(III) complexes. The structural optimization for one of the current RuIII-complexes was determined based on the processing of powder X-ray diffraction (PXRD) data by the computer program Expo 2014 PXRD. As well DFT calculations were applied to optimize the geometry in the case of complexes 1. The newly synthesized ruthenium(III) diimines were tested as catalysts for hydrogenation of cyclohexene. The effect of the catalyst structure and the type of catalysis as well as the nature and amount of the solvent used on the catalytic performance of the current catalysts were studied. Catalytic experiments reported that the ongoing ruthenium(III) complexes are promising precatalysts that have successfully catalyzed hydrogenation of cyclohexene by hydrogen gas under moderate process conditions. The results obtained allowed to establish a mechanism for the studied catalytic hydrogenation reactions.
Rationale and Design of the Application Value of Beijing Vascular Health Stratification (BVHS) : Predictive Value of Combined Assessment of Vascular Structure and Function for Cardiovascular Events in General Chinese Population
Authors: Liu Huan , Zhou Feng , Liu jinbo , huang wei , Zhao na , Zhou xiaohua , Wang hongyu 
Published: April 2021

Abstract
Vascular endothelial dysfunction, arteriosclerosis and atherosclerotic plaque are well-known risk factors for cardiovascular disease (CVD). Studies on vascular health markers have been well-established, however, there is still a lack of related research on combined vascular structure and function indicators. Beijing vascular health stratification (BVHS) is an evaluation system aiming at vascular health, combined the endothelial function, arteriosclerosis, atherosclerotic plaque and vascular lumen stenosis to comprehensively assess the vascular health and grade it. This study will explore the predictive value of the combined evaluation of vascular structure and function for cardiovascular events and assess the predictive value of BVHS and compare it with the existing risk assessment systems. A total of 1,500 subjects will be enrolled into the prospective cohort study from a community and will be followed up for at least 3 years from July 1, 2020 to June 30, 2023. Subjects aged 40 or above, without coronary heart disease, stroke or peripheral artery disease, with written informed consent will be included; subjects with end-stage hepatorenal diseases (uremia, renal failure, cirrhosis, liver failure), mental disorders or cognitive disorders, with any other factors that the researcher thinks are not suitable for the study will be excluded. Traditional cardiovascular risk factors will be collected as adjusted confounders. BVHS is a potential and scientific vascular health evaluation system. The study will be the first to grade vascular health by combing various vascular indicators and explore the prediction value and compare with other risk prediction system in general Chinese population.
Effect of Salt Stress on Some Physio-Biochemical Traits and Antioxidative Enzymes of Two Brassica Species Under Callus Culture
Authors: Shahbazi Ehsan , Jamei Shima , Meratan Ali Akbar , Mohammadi Payam Pour 
Published: April 2021

Abstract
The changes in lipid peroxidation, H2O2, proline, protein and the involvement of the different antioxidant system (catalase, gayacoule peroxidase, ascorbate peroxidase) and callus-related traits were investigated in relation to salt stress in the callus of two different ploidy levels of Brassica including B. juncea and B. oleracea. The calluses of B. juncea genotypes were less sensitive to NaCl stress than that of B. oleraceae by increasing concentrations of NaCl from 0 to 200 mM.Tetraploid genotype (B. juncea cr3356) showed significant increase in the contents of protein and proline, and also activity of giacle peroxidase and catalase enzymes at higher salinity levels. Also, a significant decrease in the amount of H2O2 and malondialdehyde occurred with increasing the salinity intensity. Diploid cultivar (B. oleracea bra 2828) had the lowest enzymatic activities and the highest content of H2O2 and malondialdehyde with an increase in the salinity level. Therefore, this genotype was identified as the most sensitive cultivar to the salinity stress. The difference in the salinity resistance between diploid and amphidiploid species may be due to differences in the ploidy level of these species. This result suggests that tetraploid genome of B. oleraceae could be considered as a suitable candidate for production under salinity conditions by maintaining higher activities of antioxidant enzymes.
Prevalence, Incidence, and Epidemic Trends of Osteoarthritis Among Adults in Beijing, China, From 2008 to 2017
Authors: Chen Hongbo , Wu Junhui , Wang Zijing , Wu Yao , Wu Tao , Wu Yiqun , Wang Mengying , Wang Siyue , Wang Xiaowen , Wang Jiating , Yu Huan , Hu Yonghua , Shang Shaomei 
Published: April 2021

Abstract
Objective

To estimate the prevalence, incidence, and epidemic trend of diagnosed osteoarthritis from 2008 to 2017 in Beijing, China.
Methods

This was a retrospective serial cross-sectional study. We used health-insurance claims of 17.7 million adults for 2008–2017 to identify people over 18 years-old with osteoarthritis. The population data for Beijing from China’s 6th national census were applied to standardize the results. Trends in prevalence and incidence were analyzed using joinpoint regression.
Results

We identified 2,793,467 people with osteoarthritis between 2008 and 2017, 60% of which were women. The 10-year average age-standardized prevalence and incidence of osteoarthritis in Beijing was, respectively, 5.2% and 28.5 per 1000 person-years. Prevalence increased with age, surging after 55 years-old. The average crude prevalence for this decade was 14.9% for people over 55 years-old: 10.7% for males and 20.4% for females. The prevalence showed an increasing trend from 2008 to 2017, including a period of rapid rise, with an annual percentage change of 44.3% from 2008 to 2011 (P༜0.05); the increase in prevalence was greatest in people under 35 years-old, with the an average annual percentage change of 36.1% (P < 0.05).
Conclusion

We observed that the average age-standardized prevalence of diagnosed osteoarthritis in Beijing per decade was at a low-to-medium level compared to worldwide levels. Annual prevalence increased significantly from 2008 to 2017, with osteoarthritis being diagnosed at younger ages. More public health efforts are needed to prevent osteoarthritis in China.
Power Transmission and Channel Control Approach using Learning Automata in the Internet of Things
Authors: Chen Peilin 
Published: April 2021

Abstract
Energy consumption management and optimal use of node resources are key elements in the Internet of Things. In this study, a framework based on cognitive topology control that acts based on the LR−I learning automata and game on it, has been used to control power, channel and contention windows and to create preventive behavior in the network. Due to the limitations of nodes in the Internet of Things, the transfer of learning automata processing to the cloud, fog and edge has been investigated to increase lifespan, reduce memory consumption and increase processing power. The communication was done based on IPv6 protocol and IEEE 802.15.4 standard. The nodes also used the uIP lightweight protocol stack and the RPL lightweight routing protocol. In order to use the sixth version of the Internet Protocol in the IEEE 802.15.4 standard platform, the 6LoWPAN protocol has been used to compress and convert headers. Computing on fog nodes has also been used to perform game calculations on automata. Finally, the Cooja simulator was used in the Contiki operating system to evaluate the efficiency of the proposed method, which showed the superiority of the proposed method in energy consumption, memory usage and processing power compared to other methods that control power and channel.