bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Analysis of NAC Transcription Factors in Sunflower (Helianthus Annuus), Their Comparative Phylogenetic Analysis and Association With Leaf Senescence
Authors: Luoni Sofia A. Bengoa , Cenci Alberto , Moschen Sebastian , Nicosia Salvador , Radonic Laura M. , Garcia Julia Sabio y , Langlade Nicolas B. , Vile Denis , Rovere Cecilia Vazquez , Fernandez Paula 
Published: September 2021

Abstract
Background Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. ResultsTo clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate.ConclutionsThis finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.
Neuronal Ceroid Lipofuscinosis Type 6 (CLN6) Clinical Findings and Molecular Diagnosis: Costa Rica´s Experience
Authors: Badilla-Porras Ramses , Echeverri-McCandless Ann , Weiner Jill , Ulate-Campos Adriana , Soto-Rodríguez Andrés , Gutiérrez-Mata Alfonso , Hernández-Con Laura , Bogantes-Ledezma Sixto , Balmaceda-Meza Andrea , Brudvig Jon , Sanabria-Castro Alfredo 
Published: September 2021

Abstract
Background: Commonly known as Batten disease, the neuronal ceroid lipofuscinoses (NCLs) are a genetically heterogeneous group of rare pediatric lysosomal storage disorders characterized by the intracellular accumulation of autofluorescent material (known as lipofuscin), progressive neurodegeneration, and neurological symptoms. In 2002, a disease-causing NCL mutation in the CLN6 gene was identified (c.214G>T) in the Costa Rican population, but the frequency of this mutation among local Batten disease patients remains incompletely characterized, as do clinical and demographic attributes for this rare patient population.Objective: To describe the main sociodemographic and clinical characteristics of patients with a clinical diagnosis for Batten Disease treated at the National Children's Hospital in Costa Rica and to genetically characterize their causative mutations.Methods: CLN6 gene sequences were determined from DNA extracted from patient buccal swabs. Participants’ sociodemographic and clinical characteristics were also obtained from their medical records.Results: Nine patients with a clinical diagnosis of Batten disease were identified. Genetic sequencing determined the presence of the previously-described Costa Rican homozygous mutation in 8 of 9 cases. One patient did not have mutations in the CLN6 gene. In all cases where the Costa Rican CLN6 mutation was present, it was accompanied by a substitution in intron 2. Patients were born in 4 of the 7 Costa Rican provinces, with an average onset of symptoms close to 4 years of age. No parental consanguinity was present in pedigrees. Initial clinical manifestations varied between patients but generally included: gait disturbances, language problems, visual impairment, seizures and psychomotor regression. Cortical and cerebellar atrophy was a constant finding when neuroimaging was performed. Seizure medication was a common element of treatment regimens.Conclusions: This investigation supports that the previously characterized c.214G>T mutation is the most common causative NCL mutation in the Costa Rican population. This mutation is geographically widespread among Costa Rican NCL patients and yields a clinical presentation similar to that observed for CLN6 NCL patients in other geographies.
Clinical Impact of Pneumothorax in Patients with Pneumocystis Jirovecii Pneumonia and Respiratory Failure in An HIV-Negative Cohort
Authors: Choi Ji Soo , Kwak Se Hyun , Kim Min Chul , Seol Chang Hwan , Kim Sung Ryeol , Park Byung Hoon , Lee Eun Hye , Yong Seung Hyun , Leem Ah Young , Kim Song Yee , Lee Sang Hoon , Chung Kyung Soo , Kim Eun Young , Jung Ji Ye , Kang Young Ae , Park Moo Suk , Kim Young Sam , Lee Su Hwan 
Published: September 2021

Abstract
Background: Pneumocystis jirovecii pneumonia (PCP) with acute respiratory failure can result in development of pneumothorax during treatment. This study aimed to identify the incidence and related factors of pneumothorax in patients with PCP and acute respiratory failure and to analyze their prognosis.Methods: We retrospectively reviewed the occurrence of pneumothorax, including clinical characteristics and results of other examinations, in 119 non-human immunodeficiency virus patients with PCP and respiratory failure requiring mechanical ventilator treatment ina medical intensive care unit (ICU) at a tertiary-care center between July 2016 and April 2019.Results: The 28-day survival rate was 62.2% (N=74). Twenty-two patients (18.5%) developed pneumothorax during ventilator treatment, with 45 (37.8%) eventually requiringa tracheostomy due to weaning failure. Cytomegalovirus co-infection (odds ratio 13.9; p=0.013) was related with occurrence of pneumothorax. Of 74 patients with 28-day survival data, 15 (20.3%) developed pneumothorax. In those patients, five survived (p=0.048) and only two were successfully weaned from mechanical ventilation (p=0.037).Conclusions: Patients with PCP and acute respiratory failure who developed pneumothorax did not have increased 28-day mortality, however pneumothorax increased in patient with cytomegalovirus co-infection, pneumothoraxmight have difficulty in and prolonged weaning from mechanical ventilators, which clinicians should be aware of when planning treatment for such patients.
A Qualitative Exploration of “Empathic Labor” in Chinese Hospice Nurses
Authors: Wang Ya-Ling , Yang Zi-Wei , Tang Yue-Zhong , Li Hui-Ling , Zhou Lan-Shu 
Published: September 2021

Abstract
Background: Hospice nurses may devote more emotional labor during the empathy process with patients, and this empathy can be used as a form of psychological behavior of emotional labor in the hospice care model. The aim of this study was to analyze hospice nurses’ empathy characteristics in the context of emotional labor theory, and explore the impact of empathy on patient care.Method: We conducted semi-participant observations from three hospitals and multicenter in-depth interviews with n=26 hospice nurses from eight cities. Interviews were transcribed, and directed content analysis was applied.Results: Two categories with four sub-categories were extracted from the data analysis. Category 1 described the “empathic labor” process which covers cognitive empathy (including empathic imagination, empathic consideration, and empathic perception) and affective empathy (including natural empathy, surface empathy, and deep empathy). The second category concerns the outcome of nurses’ “empathic labor” which incorporates both positive and negative effects.Conclusions: The findings indicated that hospice nurses’ empathy process should be understood as emotional labor. Nursing managers should pay more attention to raising the ability of deep empathy with hospice nurses, and explore more sufficient active empowerment strategies to alleviate the negative impact of empathy on nurses and to strengthen nurses' deep empathy with terminal patients.
Molecular Characterization of a Natural Mutant of Feline Calicivirus in China
Authors: Li Hang , Wang Yuanhong , Chen Zongyan , Meng Chunchun , Liu Guangqing , Li Chuanfeng 
Published: September 2021

Abstract
During epidemiological surveillance of Feline calicivirus (FCV) isolates in Shanghai, China, a natural mutant of FCV, designated SH1909, was successfully isolated from a stray cat. The complete genome sequence of SH1909 was determined in this study. Sequence comparison and analysis showed that thirteen unique aa residues substitutions and single-aa insertion of N or Y were observed in SH1909, which indicated that SH1909 was a novel and natural mutant of FCV. Interestingly, phylogenetic analysis based on LC-VP1 showed SH1909 could be clustered into an independent evolutionary branch with some Chinese isolates and was more distantly related to vaccine strains, indicating its potential to escape from vaccine-elicited immunity. According to the predicted B-cell epitopes of LC-VP1, amino acid mutation sites and positive selective sites, peptide C (aa sites: 445–460) and, peptide D (aa sites: 425–440) located in the hypervariable regions of LC-VP1, may result in the decreased immunological protection. Moreover, amino acid sites 439 and 449 may be responsible for the potential immune escape of SH1909. This study provides an important insight into genetic variations of FCV and vaccine development.
Major Histocompatibility Complex Class I in Hippocampus Promotes the Comorbid Depressive Symptoms in Cancer Induced Bone Pain via Regulating the Microglial TREM2/DAP12 Signaling
Authors: Li Xiaohui , Jia Yifu , Xiong Mengyuan , Peng Minjing , Su Shanchun , Xu Xueqin , Wu Yanqiong , Ke Changbin 
Published: September 2021

Abstract
Pain and depression comorbidity affect the patients’ both physical and mental health and quality of life seriously. The comorbid depressive symptoms in cancer pain severely affect the recognition and treatment of pain. Similarly, cancer pain patients with depression are inclined toward more despair and greater impairment. The mechanisms responsible for the comorbid depressive symptoms in cancer induced bone pain have not been fully delineated and the currently available therapeutics for this pathological pain is relatively limited. In the present study, we observed that carcinoma cells implantation induced pain and depression comorbidity resulted in the upregulation of major histocompatibility complex class I (MHC-I) in hippocampus associated with the activation of TREM2/DAP12-mediated microglial signaling pathways. These observations were reversed by a lentiviral vector harboring RNA interference sequence targeting MHC-I injected into the hippocampus of tumor bearing mice. Together, these results suggest that MHC-I involves in the cancer induced bone pain and depression comorbidity through regulating the TREM2/DAP12-mediated signals in microglia of hippocampus. Suppression of MHC-I could be a therapeutic target for cancer induced bone pain.
Clinical Efficacy of Intra-Articular Injection With P-PRP Versus That of L-PRP in Treating Knee Cartilage Lesion: A Randomized Controlled Trial.
Authors: Zhou Yiqin , Li Haobo , Cao Shiqi , Han Yaguang , Shao Jiahua , Fu Qiwei , Wang Bo , Wu Jun , Xiang Dong , Liu Ziye , Wang Huan , Zhu Jun , Qian Qirong , Yang Xiaolei , Wang Song 
Published: September 2021

Abstract
Background: To evaluate the clinical efficacy and safety of intra-articular injection with pure platelet-rich plasma (P-PRP) versus those of leukocyte platelet-rich plasma (L-PRP) in treating knee cartilage lesions, we conducted a double-blind, randomized controlled clinical trial with a larger sample and longer follow-up period.Methods: From October 2019 to October 2020, 95 patients were invited to participate in our study, and 60 (63.2%) were randomized to P-PRP (n = 30) or L-PRP (n = 30) groups. Patients from the two groups were treated with knee intra-articular injections of P-PRP or L-PRP. Visual analog scale (VAS) and Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores were assessed preoperatively and at 6 weeks, 12 weeks, 6 months, and 12 months after intervention.Results: We followed up 27 cases in the P-PRP group and 26 cases in the L-PRP group. No significant differences in VAS and WOMAC scores were found between the two groups before the intervention (P>0.05). The WOMAC Pain and VAS-Motions scores of the P-PRP group were significantly lower than those of the L-PRP group at 6 weeks after the intervention (P<0.05). While the long-term clinical efficacy of both injections was similar and weakened after 12 months, more adverse events were found in the L-PRP group.Conclusions: The short-term results of our study are encouraging and demonstrate that both L-PRP and P-PRP intra-articular injections reduce pain and improve function in patients with knee cartilage lesions. Compared with the L-PRP injection, the P-PRP injection (which had a lower risk of early inflammation caused by leukocytes) showed better clinical efficacy in the early phase of postoperative rehabilitation and resulted in fewer adverse events. However, long-term clinical efficacy for both injections were similar and weakened after 12 months.Trial registration: ChiCTR1900026365. Registered on 3 October 2019, http://www.chictr.org.cn/showproj.aspx?proj=43911.
Quality of Medical Records in Public Health Facilities of Jimma Zone, Oromia Regional State, Southwest, Ethiopia 2021
Authors: Endashaw Nigusu , Birhanu Bezawit , Teka Melese , Abrham Gelila 
Published: September 2021

Abstract
Background: Comprehensive medical records are cornerstones in the quality and efficiency of patient care, as they can provide a complete and accurate chronology of treatments, patient results, and future plans for care. The study was aimed to assess the quality of medical records in public health facilities of Jimma Zone. Methods: A facility-based cross-sectional quantitative study design supplemented by a qualitative method was used from May 30 to July 29, 2020. A total of 384 medical records were reviewed by using the facility inventory form for quantitative data. The data was entered by EPI data 3.1 and analyzed by SPSS 23 and descriptive statistics were used to present the findings. Qualitative data were triangulated with the quantitative data.Result: The majority of the health facility has a shortage of trained and qualified recording personnel in the medical record department. Among 36 health facilities, only one facility have printer in the record room and 3(8%) of them have tracer card. The overall quality of medical records in terms of content completeness as per the standard of health facilities requirements for districts, hospitals, and health centers were 30.62%, 39.49%, 25.79% respectively. Conclusion: The majority of medical records had poor completeness of administrative data, clinical, financial, and legal contents. The overall quality of medical records in Jimma Zone was very low for components of the quality of medical records as per the standard of health facilities requirements.
Klebsiella Pneumoniae Metabolic Regulation and its Application in 1,3-Propanediol Production
Authors: Wang Yadong , Mu Jing , Liao Yonghong , Wang Yuxuan , Yin Xian , Xu Bo , Wang Fenghuan 
Published: September 2021

Abstract
Klebsiella pneumoniae is a well-known model organism for glycerol metabolism to produce 1,3-propanediol (1,3-PD), a valuable chemical intermediate for materials, such as polyesters. However, the relatively low conversion rate and productivity, as well as the accumulation of by-products such as lactic acid, ethanol and acetic acid, inhibit the production of 1,3-PD. Hereby, the 1,3-PD metabolism in K. pneumoniae was regulated through pathway engineering by using CRISPR-Cas9 technology for the first time to knock out the ldhA gene of lactate dehydrogenase,the adhE gene of alcohol dehydrogenase and the ack gene of acetate kinase respectively as needed and constructed recombinant bacteria ldhA(−), ldhA(−)-ack(−), ldhA(−)-adhE(−) and ldhA(−)-adhE(−)-ack(−), all of which showed a decrease in by-product production, leading to a higher NADH availability, and 1,3-BD production was significantly increased. In the shake flask fermentation, the 1,3-PD yield and conversion rate of the recombinant strain ldhA(−), ldhA(−)-ack(−), ldhA(−)-adhE(−), ldhA(−)-adhE(−)-ack(−) were higher than those of the parent strain. In the fed-batch fermentation, the 1,3-PD yield and conversion rate of the recombinant strain ldhA(−) were higher than those of the parent strain. The biomass of the recombinant strain ldhA(−)-adhE(−)-ack(−) was reduced due to the accumulation of acetic acid, but its 1,3-PD conversion rate was still higher than that of the parent strain. The higher productivity and fewer by-products concluded that the four Klebsiella pneumoniae recombinant strains could be promising industrial strain for economical production of 1,3-PD.
Cryptogenic Cirrhosis may be Nonobese Nonalcoholic Steatohepatitis—Risk Factors of Liver Steatosis After Liver Transplantation for Cryptogenic Cirrhosis: A Retrospective Study
Authors: Fukushima Masanori , Miyaaki Hisamitsu , Sasaki Ryu , Haraguchi Masafumi , Miuma Satoshi , Hara Takanobu , Soyama Akihiko , Hidaka Masaaki , Eguchi Susumu , Nakao Kazuhiko 
Published: September 2021

Abstract
Background

As the diagnosis of non-alcoholic steatohepatitis (NASH) has become more accurate, the number of patients with cryptogenic cirrhosis (CC) has decreased. Despite this, there are patients in the liver transplant registry who are currently listed as having CC. Therefore, knowledge of the natural course of CC after liver transplantation (LT) is important. However, the natural course of CC after LT is unknown. We aimed to clarify the natural course post LT for CC and to infer the etiology of CC.
Methods

Eighteen patients who underwent LT for CC were included. To rule out the possibility of including NASH patients with CC patients, those with obesity (body mass index [BMI] ≥ 25 kg/m2) and type-2 diabetes mellitus or liver steatosis found on pretransplantation images, were excluded. Liver biopsy was performed 1 year after LT, and annually thereafter.
Results

Liver steatosis and steatohepatitis were identified in 61% and 39% of the patients after LT, respectively with a median time to onset of 12 and 27 months, respectively. No other pathological findings could identify the etiology of CC. The BMIs after LT were significantly higher in the steatosis group than in the non-steatosis group (28.5 vs. 22.4 kg/m2; P = 0.002). The mean muscle attenuation (MA) at the time of LT was significantly higher and the postoperative hospitalization period was shorter in the steatosis group than in the non-steatosis group (MA: 33.3 vs. 25.8 Hounsfield unit, P = 0.03; hospitalization: 50 vs. 102 days, P = 0.02). Recipients were significantly younger in the steatohepatitis subgroup than in the simple steatosis subgroup (55.0 vs. 63.5 years, P = 0.04).
Conclusions

Despite excluding patients with a history of obesity from our cohort of patients with CC, we observed that patients with CC had a high prevalence of steatosis after LT. Young patients with a favorable postoperative course were noted to have a high risk of NASH after LT for CC. Patients with CC may represent cases of non-obese NASH.
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase
Authors: Liu Jiawen , Liu Cong , Qiao Shilei , Dong Zhen , Sun Di , Zhu Jingrong , Liu Weijie 
Published: September 2021

Abstract
One-step fermentation is a cheap way to produce xylo-oligosaccharides (XOS), where production of xylanases and XOS is integrated into a single process. In spite of having cost advantage, one-step fermentation is still short in yield so far due to the limited exploration. To cope with this issue, production of XOS from wheat bran by recombinant Escherichia coli through one-step fermentation was investigated here. A xylanase gene belonging to glycoside hydrolase family 11 of Bacillus agaradhaerens was employed to construct recombinant E. coli. This xylanase showed maximal activity at 60°C and pH 8.0. Its activity retained more than 60% after incubation at 70°C for 4 hours, showing a good stability. The recombinant E. coli successfully secreted xylanases that directly hydrolyzed wheat bran to XOS in fermentation medium. The generated XOS consisted of xylose, xylobiose and xylotriose accounting for 23.1%, 37.3% and 39.6%, respectively. Wheat bran concentration was found to be the most crucial factor affecting XOS production. The yield reached 5.3 mg/mL at 10% of wheat bran, which is higher than previous reports employing one-step fermentation. Nitrogen source type could also affect XOS yield by changing extracellular xylanase activity, and glycine was found to be the best one for fermentation. Optimal fermentation conditions were finally studied by response surface optimization. The maximal yield emerged at 44.3°C, pH 7.98, which is affected by characteristics of the xylanase and growth conditions of E. coli. This work indicates that the integrated fermentation using recombinant E. coli is highly competitive in cost and yield for production of XOS.
A Combined Model Based on Society Cognitive Optimization Algorithm for Wind Speed Forecasting
Authors: He Zhaoshuang , Chen Yanhua , Li Min 
Published: September 2021

Abstract
Wind energy, as renewable energy, has drawn the attention of society. The use of wind power generation can reduce the pollution to the environment and solve the problem of power shortage in offshore islands, grassland, pastoral areas, mountain areas, and highlands. Wind speed forecasting plays a significant role in wind farms. It can improve economic and social benefits and make an operation schedule for wind turbines in large wind farms. At present, researchers have proposed a variety of methods for wind speed forecasting; artificial neural network (ANN) is one of the most commonly used methods. This paper proposes a combined model based on the existing artificial neural network algorithms for wind speed forecasting at different heights. We first use the wavelet threshold method to the original wind speed data set for noise reduction. After that, the three artificial neural networks, extreme learning machine (ELM), Elman neural network, and Long Short-Term Memory neural network (LSTM), are applied for wind speed forecasting. In addition, variance reciprocal method and society cognitive optimization algorithm (SCO) are used to optimize the weight coefficients of the combined model. In order to evaluate the forecasting performance of the combined model, we select wind speed data at three heights (20m, 50m, and 80m) in National Wind Technology Center M2 Tower. The experimental results show that the forecasting performance of the combined model is better than the single model, and it has a good forecasting performance for the wind speed at different heights.