bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Closing the Abdominal Donor-Site Superficial Fascial System in Deep Inferior Epigastric Perforator Flap Breast Reconstruction
Authors: Johnson Ariel C. , King Becky B. T. , Colakoglu Salih , Yang Jerry H. , Chong Tae W. , Mathes David W. 
Published: August 2021

Background:
The superficial fascial system is routinely closed to alleviate tension at the abdominal donor site after harvest of the deep inferior epigastric artery perforator flap (DIEP) for breast reconstruction. This is thought to decrease rates of wound dehiscence and improve contour postoperatively. There has been no comparative analysis on closure of the superficial fascial system and its effect on donor-site outcomes.

Methods:
The authors retrospectively evaluated outcomes of DIEP flap breast reconstructions performed between 2017 and 2019. After May of 2018, the surgeons collectively agreed to stop closure of the superficial fascial system. All subsequent patients underwent closure of rectus abdominis fascia followed by skin closure. Patient demographic data and abdominal donor-site comorbidities were recorded between the superficial fascial system closure and no–superficial fascial system closure groups. Representative photographs of patients from the two groups were blindly assessed for scar appearance and contour using previously published grading scales. The results were compared.

Results:
DIEP flap breast reconstruction was performed in 103 consecutive women. Among patients with abdominal donor-site reconstruction, 66 had superficial fascial system closure and 37 did not. There was not a significant difference in fat necrosis or wound dehiscence between the two groups (p = 0.29 and p = 0.39, respectively). Postoperative abdominal scar and contour were evaluated by 10 independent raters and showed no significant difference between the two groups.

Conclusion:
Omission of superficial fascial system closure resulted in no difference in wound dehiscence or fat necrosis rates and aesthetic appearance of the abdominal scar and contour.

Clinical Question/Level of Evidence:
Therapeutic, III.
Transcriptional plasticity drives leukemia immune escape
Authors: Eagle Kenneth , Harada Taku , Kalfon Jérémie , Perez Monika W. , Heshmati Yaser , Ewers Jazmin , Koren Jošt Vrabič , Dempster Joshua M. , Kugener Guillaume , Paralkar Vikram R. , Lin Charles Y. , Dharia Neekesh V. , Stegmaier Kimberly , Orkin Stuart H. , Pimkin Maxim 
Published: August 2021

ABSTRACTRelapse of acute myeloid leukemia (AML) after allogeneic bone marrow transplantation (alloSCT) has been linked to immune evasion due to reduced expression of major histocompatibility complex class II (MHC-II) proteins through unknown mechanisms. We developed CORENODE, a computational algorithm for genome-wide transcription network decomposition, that identified the transcription factors (TFs) IRF8 and MEF2C as positive regulators and MYB and MEIS1 as negative regulators of MHC-II expression in AML cells. We show that reduced MHC-II expression at relapse is transcriptionally driven by combinatorial changes in the levels of these TFs, acting both independently and through the MHC-II coactivator CIITA. Beyond the MHC-II genes, MYB and IRF8 antagonistically regulate a broad genetic program responsible for cytokine signaling and T-cell stimulation that displays reduced expression at relapse. A small number of cells with altered TF levels and silenced MHC-II expression are present at the time of initial leukemia diagnosis, likely contributing to eventual relapse. Our findings reveal an adaptive transcriptional mechanism of AML evolution after allogenic transplantation whereby combinatorial fluctuations of TF levels under immune pressure result in selection of cells with a silenced T-cell stimulation program.
Procalcitonin to Predict Severity of Acute Cholangitis and Need for Urgent Biliary Decompression: Systematic Scoping Review
Authors: Silangcruz Krixie , Nishimura Yoshito , Czech Torrey , Kimura Nobuhiko , Yess James 
Published: August 2021

AbstractBackgroundSerum Procalcitonin (PCT) has been reported as a potential biomarker to predict the severity of acute cholangitis (AC) or the need for urgent biliary decompression. This study aimed to identify and summarize the existing research about the serum PCT and the severity of AC, and to find gaps where future studies can be targeted towards.MethodsFollowing the PRISMA Extension for Scoping Reviews, MEDLINE, EMBASE, and Google Scholar were searched for all peer-reviewed articles with relevant keywords including “cholangitis” and “procalcitonin” from their inception to July 13, 2021.ResultsWe identified six studies. All the studies employed case-control design and aimed to evaluate the usefulness of serum PCT to predict the severity of AC with key identified outcomes. While potential cut-off values of serum PCT for severe AC ranged from 1.8–3.1 ng/mL, studies used different severity criteria and the definition of urgent biliary decompression. No studies proposed cut-off PCT values for the need for urgent biliary decompression.ConclusionThis scoping review identified that the current level of evidence regarding the usefulness of serum PCT in assessing the severity of AC. Further clinical research is warranted with a focus on standardized outcome measures employing prospective or experimental designs.
Structural mechanism underpinning Thermus oshimai Pif1-mediated G-quadruplex unfolding
Authors: Dai Yang-Xue , Guo Hai-Lei , Liu Na-Nv , Chen Wei-Fei , Rety Stephane , Xi Xu-Guang 
Published: August 2021

ABSTRACTG-quadruplexes (G4s) are unusual DNA structures and can stall DNA replication, causing genomic instability for the cell. Although the solved crystal structure of the DHX36 helicase demonstrated that G4 was specifically targeted by a DHX36-specific motif (DSM), lack of complete structural details for general G4-resolving helicases without specific target motifs remains a barrier to the complete understanding of the molecular basis underlying the recognition and unfolding of G4s. Herein, we present the first X-ray crystal structure of the Thermus oshimai Pif1 (ToPif) complexed with a G4, thereby mimicking the physiological G4 formed during DNA replication. Strictly different from the previous determined G4-helicase structure of DHX36, our structure revealed that ToPif1 recognizes the entire native G4 via a cluster of amino acids at domains 1B/2B constituting a G4-Recognizing Surface (GRS). The overall topology of the G4 structure solved in this work maintains its three-layered propeller-type G4 topology, with no significant reorganization of G-tetrads upon protein binding. The three G-tetrads in G4 were differentially recognized by GRS residues mainly through electrostatic, ionic interactions and hydrogen bonds formed between the GRS residues and the ribose-phosphate backbone. Our structure explains how helicases from distinct superfamilies adopt different strategies for recognizing and unfolding G4s.
A dynamic rhizosphere interplay between tree roots and soil bacteria under drought stress
Authors: Oppenheimer-Shaanan Yaara , Jakoby Gilad , Starr Maya Laurencia , Karliner Romiel , Eilon Gal , Itkin Maxim , Malitsky Sergey , Klein Tamir 
Published: August 2021

AbstractRoot exudates are thought to play an important role in plant-microbial interactions. In return for nutrition, soil bacteria can increase the bioavailability of soil minerals. However, root exudates typically decrease in situations such as drought, calling into question the efficacy of bacteria-dependent mineral uptake in such stress.Here we tested the hypothesis of exudate-driven microbial priming on Cupressus saplings grown in forest soil in custom-made rhizotron boxes. A 1-month imposed drought and concomitant inoculations with Bacillus subtilis and Pseudomonas stutzeri, bacteria species isolated from the forest soil, were applied using factorial design.Direct bacteria counts and visualization by confocal microscopy showed that both bacteria associated with Cupressus roots. Interestingly, root exudation rates increased with bacteria under drought. Forty four metabolites in exudates were significantly different in concentration between irrigated and drought trees, including phenolic acid compounds and quinate, that were shown to be used as carbon and nitrogen sources by both bacterial species. Importantly, soil phosphorous bioavailability was maintained only in inoculated trees, mitigating drought-induced decrease in leaf phosphorus and iron.Our observations of increased root exudation rate when drought and inoculation regimes were combined, support the idea of root recruitment of beneficial bacteria.
Nucleosome positioning on large tandem DNA repeats of the ‘601’ sequence engineered in Saccharomyces cerevisiae
Authors: Lancrey Astrid , Joubert Alexandra , Duvernois-Berthet Evelyne , Routhier Etienne , Raj Saurabh , Thierry Agnès , Sigarteu Marta , Ponger Loic , Croquette Vincent , Mozziconacci Julien , Boulé Jean-Baptiste 
Published: August 2021

AbstractThe so-called 601 DNA sequence is often used to constrain the position of nucleosomes on a DNA molecule in vitro. Although the ability of the 147 base pair sequence to precisely position a nucleosome in vitro is well documented, in vivo application of this property has been explored only in a few studies and yielded contradictory conclusions. Our goal in the present study was to test the ability of the 601 sequence to dictate nucleosome positioning in Saccharomyces cerevisiae in the context of a long tandem repeat array inserted in a yeast chromosome. We engineered such arrays with three different repeat size, namely 167, 197 and 237 base pairs. Although our arrays are able to position nucleosomes in vitro as expected, analysis of nucleosome occupancy on these arrays in vivo revealed that nucleosomes are not preferentially positioned as expected on the 601-core sequence along the repeats and that the measured nucleosome repeat length does not correspond to the one expected by design. Altogether our results demonstrate that the rules defining nucleosome positions on this DNA sequence in vitro are not valid in vivo, at least in this chromosomal context, questioning the relevance of using the 601 sequence in vivo to achieve precise nucleosome positioning on designer synthetic DNA sequences.
Capabilities to withstand vulnerabilities and boost resilience in industrialized construction supply chains: a Hong Kong study
Authors: Ekanayake E.M.A.C. , Shen Geoffrey , Kumaraswamy Mohan , Owusu Emmanuel Kingsford , Xue Jin 
Published: August 2021

PurposeGiven the heightened imperatives for boosting supply chain resilience (SCR) in industrialized construction (IC), it is essential to explore the correlational impacts of supply chain vulnerabilities (SCV) and supply chain capabilities (SCC) which are the measures of SCR, specifically in Hong Kong where policymakers actively promote IC. Therefore, this study aims to develop a model to explore the correlational impacts of vulnerabilities and capabilities targeting SCR in IC.Design/methodology/approachAfter drawing on the general literature on SCR, empirical research using an expert opinion survey was conducted following the methodological framework of this study. The gathered data were then subjected to the partial least squares structural equation modeling process. Thereby, four hypotheses were formulated and tested for 20 capability–vulnerability relationships.FindingsSeven of the 20 statistical relationships tested were identified to be significant. Accordingly, production-based SCV were identified as the most critical disruptions. “Resourcefulness” could substantially withstand production-based SCV, receiving the highest path significance. An “enablers-results framework” for achieving SCR of IC was also developed based on these findings to help industry practitioners with SCR implementation.Originality/valueTo the authors' knowledge, this is the first structured evaluation model that measures the correlational impacts of SCC and SCV targeting SCR in the construction domain. Further, this study adds substantially to the existing SCR and construction “body of knowledge” by proposing a model explaining how various SCV and SCC influence SCR in IC. These findings also inform the industry where and how to deploy critical SCC at appropriate levels, targeting critical SCV, to contain or extirpate them.
Impact of client vendor communication on client satisfaction in information system based projects
Authors: Rasool Zeeshan , Xue Jian , Awesi Javeria , Abbas Syed Arslan , Farooq Zahid 
Published: August 2021

PurposeThe research focuses on the impact of client vendor communication on client satisfaction in information system projects and after client satisfaction that will bridge them toward the information system continuance intention of clients. Importance of this study is to understand the effect of client-vendor communication on client satisfaction.Design/methodology/approachThe sample size of the study was 200 respondents and data was collected from electrical manufacturing industries. It is a quantitative field study, and the time horizon of this study is cross-sectional. The data collected was then analyzed by doing the structural equation modeling in PLS-SEM in order to examine the relationships.FindingsFindings of this study likewise demonstrate the mediating impact of client satisfaction between the relationship of client-vendor communication and information system continuance intention as well as between the relationship of expectation confirmation and information system continuance intention. Future studies may include the multi-motive information system continuance model (MISC) to explain the constructs in more depth because it includes different dimensions of expectation confirmation which will help more to understand information system continuance intention.Originality/valueThis study determines the role of information system continuance intention in terms of client-vendor communication and expectation confirmation through the impact of mediator client satisfaction. Previous studies have explained that client-vendor communication affects client satisfaction positively and confirmation also positively affects the client satisfaction. For this research study, the authors have identified the following research gaps. The authors also have found out that the following study is significant in a related study taken in the developed countries. On the contrary, the authors will follow the study and further observe the impact of improved risks in different cities of Pakistan that how client-vendor communication are affecting the success of information
A holistic model for measuring continuous innovation capability of manufacturing industry: a case study
Authors: Lianto Benny , Dachyar Muhammad , Soemardi Tresna Priyana 
Published: August 2021

PurposeThe purpose of this paper is to develop a comprehensive continuous innovation capability (CIC) measurement model in manufacturing sectors.Design/methodology/approachThe development of this CIC model was conducted through three stages of research, i.e. identification of manufacturing continuous innovation measures (MCIMs), development of measurement model, followed by model evaluation and validation. MCIMs were identified using systematic literature review and focus group discussion. Selection process for MCIMs employed the fuzzy Delphi method. To develop measurement model, contextual relationships between MCIMs were assessed using total interpretive structural modeling, followed by measurements of MCIMs weight with the analytical network process method. Then, assessment indicators for each MCIM and criteria were determined as well as mathematical model to measure CIC scores. Model evaluation and validation were performed in two case studies: in an automotive company and an electronics company.FindingsThis research produced 50 criteria and 103 assessment indicators, as well as mathematical model to measure CIC scores. The validation process showed that currently developed model was deemed valid.Practical implicationsThe results of this research are expected to provide a practical input for manufacturing company managers in managing their innovation activities systematically and comprehensively.Originality/valueThe CIC model is a new comprehensive measurement model; it integrates three fundamental elements of CI capability measurement, considering all important dimensions in a company and also able to explain contextual relationships between measured factors.
Hybridization in a kibbutz industry structure: an Israeli case study
Authors: Moskovich Yaffa 
Published: August 2021

PurposeThis article analyzes a kibbutz factory and seeks to understand its unique hybrid structure following privatization, comparing it with that of other kibbutz industries in Israeli society.Design/methodology/approachThe research used qualitative investigation, including interviews and document analysis practice.FindingsThe study describes hybrid model that is based on conflicting logic, as the kibbutz industry contains both communal and familial principles and bureaucratic and business features. This case study succeeded in striking a balance between the two conflicting logics through sound managerial policy adapted for the sake of communal interests.Practical implicationsThis typology can be applied to other business organizations that underwent organizational changes as well.Originality/valueThe authors developed an alternative hybrid organization typology capable of describing new trends in kibbutz industry.
Understanding post-adoption behaviour in the context of ride-hailing apps: the role of customer perceived value
Authors: Ofori Kwame Simpe , Anyigba Hod , Adeola Ogechi , Junwu Chai , Osakwe Christian Nedu , David-West Olayinka 
Published: August 2021

PurposeDespite the perceived role of customer value in post-adoption behaviour in the context of ride-hailing apps such as Uber, there has been limited research on the subject. This paper seeks to enrich the understanding of the relationships between customer perceived value, particularly hedonic value and economic value, customer satisfaction and continued use intentions of ride-hailing apps.Design/methodology/approachThis analysis is based on field data collected from 567 users of ride-hailing apps in Ghana. Data collected from the survey were analysed using the partial least square (PLS) approach to structural equation modelling (SEM).FindingsThe paper provides evidence that hedonic value, as well as economic value, positively predicts customer satisfaction and continued use intentions of ride-hailing apps. Further analysis reveals customer satisfaction directly predicts continued use intentions in addition to partially mediating the influence of customer perceived value on continued use intentions of ride-hailing apps. Finally, the findings suggest that hedonic value has a stronger impact on continued use intentions than economic value, while economic value has a greater impact on satisfaction than hedonic value.Originality/valueThe study contributes to post-adoption behaviour research by providing evidence on the relationships among the study constructs in a developing country context. Overall, the findings will stimulate future empirical debates on the subject and guide practitioners in decision-making concerning customers' usage of ride-hailing apps.
First-mover advantages and innovation success: a contingency approach
Authors: Chavez Daniel Eduardo , Chen Haipeng (Allan) 
Published: August 2021

PurposeThe purpose of this paper is to propose an overarching unifying theory where first-mover advantages are a conditional effect, not a main effect. By offering a closer look at how the firm, market and product characteristics influence the supply and demand of innovations, this research furthers our understanding of the advantages and disadvantages for first movers.Design/methodology/approachThis paper explores first-mover advantages as a conditional effect. Adopting a contingency perspective, the authors review the literature in marketing, strategic management, innovation and entrepreneurship to offer a conceptual framework putting innovation success at the core of first-mover advantages. The authors develop an inventory of propositions specifying how first-mover advantages depend on various firm features, market characteristics and product properties through their effects on the success of innovations.FindingsA conceptual framework centered around innovation success yields testable hypotheses that are coherent with extant research on first-mover advantages and reconcile the seemingly contradictory evidence in that body of work.Practical implicationsThis research provides managers with the opportunity to think about one of the most important decisions, i.e. time of entry, not as a linear finite decision, but instead as a flow with the innovations and potential for their success in mind.Originality/valueThis paper distinguishes itself from the existing literature with its focus on innovation within a contingency perspective for first-mover advantages.
Key account management and value co-creation in multi-stakeholder ecosystems. A “market access” mix
Authors: Leone Daniele , Schiavone Francesco , Simoni Michele 
Published: August 2021

Purpose
The present study aims to contribute to the growing stream of literature about the network perspective of value co-creation via key account management (KAM) by exploring how firms, in complex industrial markets, use key account strategies to create value, not only for buyers and sellers of industrial products/services but also, more widely, for larger ecosystems of stakeholders. The research question this paper seeks to address is how the KAM approach promotes value co-creation in multi-stakeholder ecosystem.

Design/methodology/approach
To answer this research question, this study uses a qualitative research approach based on data triangulation. This study focuses on the market access (MA) strategies implemented by a multinational UK-based pharmaceutical company within the Italian multi-stakeholder health-care ecosystem over several years.

Findings
The results show that KAM in complex networks acts as a catalyst for value creation, through multiple interactions with different actors and an ad hoc configuration of five strategic levers: product performance, economic impact, institutional relationships, commercial organization and communication. These levers are able to unlock the appropriate value drivers and form a specific “market access mix” implemented by the firm to both promote the adoption of the firm’s products and generate value for all market stakeholders.

Originality/value
The study offers an innovative and comprehensive evidence-based model for designing specific MA strategies aimed at co-creating value within multi-stakeholder ecosystems. The proposed MA mix outlines the fact that knowledge, relationships and innovation are not unique factors that can be leveraged by stakeholders to co-create value.