bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH PENDIDIKAN KEWIRAUSAHAAN, LINGKUNGAN KELUARGA TERHADAP INTENSI BERWIRAUSAHA DENGAN SIKAP BERWIRAUSAHA SEBAGAI VARIABEL MEDIASI
Authors: Prawoto Eko , Affandi Achmad 
Published: February 2021

Pada penelitian ini ditujukan untuk membuktikan efek mediasi sikap berwirausaha pada pengaruh pendidikan kewirausahaan, lingkungan keluarga, terhadap intensi berwirausaha. Responden yang dijadikan sampel adalah mahasiswa FEB Unsiq Jawa tengah di Wonosobo sebanyak 100 sampel. Analisis SEM dengan PLS digunakan untuk menguji hipotesis. Hasil penelitian ini menunjukkan intensi berwirausaha dipengaruhi oleh Pendidikan (H1 diterima). Lingkungan keluarga mempunyai pengaruh yang positif terhadap Intensi berwirausaha (H2 diterima). Sikap berwirausaha mempunyai pengaruh yang positif terhadap Intensi berwirausaha diterima (H3 diterima). Pendidikan kewirausahaan mempunyai pengaruh yang positif terhadap Intensi berwirausaha yang dimediasi oleh sikap (H4 diterima). Lingkungan keluarga mempunyai pengaruh yang positif terhadap Intensi berwirausaha yang dimediasi oleh sikap (H5 diterima).
NILAI-NILAI TASAWUF DALAM TRADISI KEAGAMAAN KOMUNITAS ABOGE
Authors: Nugroho Muhamad Yusuf Amin , Hidayat Muhtar S. 
Published: February 2021

Tujuan penelitian ini adalah untuk mengetahui bagaimana tradisi keagamaan dan nilai-nilai tasawuf dalam tradisi keagamaan Aboge di Desa Mudal, Kecamatan Mojotengah, Kabupaten Wonosobo; juga pemahaman penganut Aboge di desa tersebut. Pendekatan yang digunakan dalam penelitian ini adalah kualitatif, yang tujuannya adalah menggali dan mendeskripsikan makna dalam sebuah realita. Dengan menggunakan studi kasus penelitian ini bermaksud memahami memahami fenomena-fenomena sosial dari sudut perspektif partisipan.
Berdasar penelitian yang sudah dilaksanakan kami menemukan tradisi keagamaan Aboge di Desa Mudal, yakni Suronan, Ruwahan dan Sadranan, Puasa, dan Ngubeng jagad. Tradisi tersebut bukan hanya dalam rangka pelestarian tradisi nenek moyang, melainkan di dalamnya terkandung nilai-nilai luhur. Nilai-nilai tersebut berkaitan dengan tiga hal, yakni menyangkut hubungan manusia dengan alam, hubungan manusia dengan sesamanya, dan hubungan manusia dengan Sang Pencipta. Dengan kata lain, tradisi keagamaan masyarakat Aboge di Desa Mudal sangat kental dengan kandungan nilai-nilai tasawuf. Inti tasawuf adalah upaya melatih diri dari pengaruh negatif kehidupan dunia, sehingga tercermin akhlak yang mulia dan dekat dengan Tuhan. Tradisi keagamaan yang dijalankan oleh komunitas Aboge pada dasarnya memiliki tujuan bertasawuf tersebut. Dalam menjalankan tradisi keagamaannya, diperlukan perlengkapan yang khusus yang disebut ubarampe. Ubarampe tidak hanya properti tetapi merupakan simbol yang mengandang ajaran moral. Meski begitu, pengetahuan anggota komunitas aboge di desa Mudal tentang makna ritual dan tradisi masih sangat rendah. Hanya sekitar 50 persen penganut Aboge di Desa Mudal yang paham bahwa dalam tradisi-tradisi keagamaan yang mereka jalani terdapat nilai-nilai tasawuf.
Quantitative Content Parameter in the Standardization of Veratrum Aqua, Veratrum Lobelianum Bernh. Based Drug
Authors: Melnik E. V. , Belova M. V. , Tyurin I. A. , Ramenskaya G. V. 
Published: February 2021

Introduction. According to the XIV Edition of the Russian Federation State Pharmacopoeia, the quality control of the «Veratrum Lobelianum rhizome and roots» herbal substance is carried out through the determination of the alkaloid sum by means of the titration-based method. There are no selective and sensitive instrumental methods for the quantitative analysis of veratrum aqua active ingredients either. Veratrum aqua is produced from the mentioned above herbal substance. Therefore, the study of veratrum aqua alkaloid composition is relevant, as well as the development of a modern analytical method for individual alkaloid determination that can be implemented in veratrum aqua standardization.Aim. To develop an approach to the quantitative analysis in Veratrum Aqua standardization.Materials and methods. Two analytical methods were developed: one for the veratrum alkaloid determination in veratrum aqua samples by means of high performance liquid chromatography coupled with tandem mass-spectrometry (HPLC-MS/MS), another – for jervine, the main veratrum aqua alkaloid, quantitation by means of HPLC with diode-array detector (HPLC-DAD).Results and discussion. Three main alkaloids, namely jervine, protoveratrine A and protoveratrine B, were identified in veratrum aqua. Jervine was found to be the most abundant one, hence it was chosen for the further development of a more affordable HPLC-DAD method. This method was validated for specificity, linearity, accuracy and precision. Jervine concentrations were measured in seven veratrum aqua samples produced by different manufacturers.Conclusion. The highest jervine concentration among the examined samples was found to be 170 µg/ml, the lowest – 136 µg/ml. It is proposed to implement the following quantitative content parameter in veratrum aqua standardization: «Quantitative test. Jervine content should be not lessthan 136 µg/ml». This parameter is to be determined by HPLC-DAD.
Mothers’ Physical Activity in the New Millennium: A Systematic Review of the Literature
Authors: Juwono Ignatius Darma , Kun Bernadette , Demetrovics Zsolt , Szabo Attila 
Published: February 2021

Background. Physical activity is essential for health, especially in an increasingly sedentary world. Being a parent involves responsibilities that could interfere with mothers’ physical activity. Research suggests that mothers are especially vulnerable to physical inactivity. Health promoters invest substantial effort in facilitating the physical activity of women with children.
Methods. The scope of this systematic literature review is to synthesize research efforts on mothers’ physical activity and supporting interventions. Three databases were scrutinized in this review (Medline, Scopus, and PsycINFO). Articles published between 2000–2020 were selected in concordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA). Fifty-four eligible articles were included after screening 3708 articles.
Results. The results suggest that mothers exhibit lower levels of physical activity than the recommended level for adults. Lack of time, focus on childcare, limited knowledge, and insufficient spousal support emerge as the primary barriers to physical activity in mothers with children. Interventions trying to overcome these barriers vary in methods, evaluations, and effectiveness. Positive changes in mothers’ physical activity may emerge if interventions focus on increasing self-efficacy, problem-solving, goal setting, and other cognitive factors.
Conclusion. Future interventions integrating internet-based applications and involving spouses and/or partners might help mothers to increase their physical activity.
Keywords: exercise, health behavior, motherhood, sedentary, women.
Increased Exercise Effort after Artificially-Induced Stress: Laboratory-Based Evidence for the Catharsis Theory of Stress
Authors: Szabo Attila , Tóth Eliza , Kósa Lili , Laki Ádám , Ihász Ferenc 
Published: February 2021

Background. Evolution prepared humans to deal with physical challenges. Today, people encounter psychosocial stress more than physical stress. However, the physiological response to the contemporary forms of stress is still preserved as the biological evolution’s vestigial heritage. This laboratory investigation aimed to determine whether brief mental stress triggers greater innate (instinctual) effort to ‘let off steam’ than a non-challenging control condition.
Method. Using a counterbalanced within-participants laboratory design, 29 young men walked/jogged at voluntary (self-paced) effort after two conditions: a) artificially-induced mental stress comprised by the Stroop Color-Word Task, which lasted for five minutes, and b) a control session, also lasting for five minutes, in which the participants watched a video depicting the world’s ten tallest buildings.
Results. The increased arousal after mental stress was carried over into the walk or jog period, and participants worked harder, but they did not perceive exerting greater effort in contrast to the control condition.
Conclusions. These results suggest that a ‘flight or fight’ response to psychosocial stress is manifested in the form of subliminal catharsis. While larger-scale studies with more impactful stressors are needed, these preliminary results support the catharsis theory. They might open new research avenues to provide people more physical opportunities for letting off steam before the necessity of treatment with chemical substances or other behavioral therapies.
Keywords: cognitive stress, exercise, mental stress, flight or fight, physical activity, psychosocial stress.
Direct and Rapid Detection of Mycoplasma bovis in Bovine Milk Samples by Recombinase Polymerase Amplification Assays
Authors: Li Ruiwen , Wang Jinfeng , Sun Xiaoxia , Liu Libing , Wang Jianchang , Yuan Wanzhe 
Published: February 2021

This study aimed to detetct Mycoplasma bovis (M. bovis) in bovine milk quickly and directly by developing and validating isothermal recombinase polymerase amplification (RPA) assays. Targeting the uvrC gene of M. bovis, an RPA assay based on the fluorescence monitoring (real-time RPA) and an RPA assay combined with a lateral flow strip (LFS RPA) were conducted. It took 20 min for the real-time RPA to finish in a Genie III at 39°C, and 15 min were required to perform the LFS RPA in an incubator block at 39°C, followed by the visualization of the products on the lateral flow strip within 5 min. Both of the two assays showed high specificity for M. bovis without any cross-reaction with the other tested pathogens. With the standard recombinant plasmid pMbovis-uvrC serving as a template, both RPA assays had a limit of detcion of 1.0 × 101 copies per reaction, equivalent to that of a real-time PCR assay. In the 65 milk samples collected from cattle with mastitis, the M. bovis genomic DNA was detected in 24 samples by both the real-time RPA and the LFS RPA assays. The developed RPA assays could detect M. bovis in bovine milk in an efficient, convenient, and credible manner as attractive and promising tools, and the assays would be helpful in the rapid response to M. bovis infection causing bovine mastitis.
QT Prolongation in Cancer Patients
Authors: Kim Peter , Masha Luke , Olson Amanda , Iliescu Cezar , Karimzad Kaveh , Hassan Saamir , Palaskas Nicolas , Durand Jean-Bernard , Leung Cheuk Hong , Lopez-Mattei Juan 
Published: February 2021

Background: QT prolongation and torsades de pointes pose a major concern for cardiologists and oncologists. Although cancer patients are suspected to have prolonged QT intervals, this has not been investigated in a large population. The purpose of this study was to analyze the QT interval distribution in a cancer population and compare it to a non-cancer population in the same institution.Methods: The study was a retrospective review of 82,410 ECGs performed in cancer patients (51.8% women and 48.2% men) and 775 ECGs performed in normal stem cell donors (47.9% women and 52.1% men) from January 2009 to December 2013 at the University of Texas MD Anderson Cancer Center. Pharmacy prescription data was also collected and analyzed during the same time period. Correction of the QT interval for the heart rate was performed using the Bazett and Fridericia formulas.Results: After QT correction for heart rate by the Fridericia formula (QTcF), the mean and 99% percentile QTc for cancer patients were 414 and 473 ms, respectively. These were significantly longer than the normal stem cell donors, 407 and 458 ms, p &lt; 0.001, respectively. Among the cancer patients, the QTc was longer in the inpatient setting when compared to both outpatient and emergency center areas. The most commonly prescribed QT prolonging medications identified were ondansetron and methadone.Conclusion: Our study demonstrates significantly longer QTc intervals in cancer patients, especially in the inpatient setting. Frequently prescribed QT prolonging medications such as antiemetics and analgesics may have a causative role in QT prolongation seen in our cancer hospital.
Circulating Neutrophil Dysfunction in HBV-Related Acute-on-Chronic Liver Failure
Authors: Wu Wei , Sun Shanshan , Wang Yijie , Zhao Ruihong , Ren Haotang , Li Zhiwei , Zhao Hong , Zhang Yi , Sheng Jifang , Chen Zhi , Shi Yu 
Published: February 2021

Background and AimsAcute-on-chronic liver failure (ACLF) is characterized by systemic inflammation accompanied by defective anti-bacterial immunity. The role of neutrophils in immune derangement of ACLF has not been fully elucidated. This study is aimed to characterize the role of circulating neutrophils in HBV-related ACLF patients.MethodsQuantitative, phenotypic, transcriptomic, and functional alterations of circulating neutrophils were compared in ACLF and non-ACLF subjects and analyzed for associations with short-term outcomes. Interventional experiments were performed to test the impact on ACLF-patient neutrophil function in vitro.ResultsCirculating absolute neutrophil count was significantly increased in patients with ACLF and was an independent risk factor for 28-day mortality. ACLF-patient neutrophils differentially expressed a panel of surface markers (include TLR-1, TLR-2, TLR-4, CEACAM-1 and FPR1), as well as a distinct transcriptomic signature. ACLF-neutrophils displayed significantly impaired phagocytosis but an increased capacity to form neutrophil extracellular traps (NETs), which was more pronounced in patients with poor outcome. Healthy neutrophils mimicked functional characteristics of ACLF counterpart after co-cultured with plasma from ACLF patients. The oxidative burst and cytokine production capacities remained unchanged. Plasma GM-CSF, IL-6, IL-8, IL-10, and IP-10 levels, as well as lipopolysaccharide (LPS) concentration, were markedly elevated in ACLF patients but not DAMP molecules HMGB-1 and HSP70. Finally, a glycolysis inhibitor, 2-deoxy-glucose, reduced NET formation of ACLF patients’ neutrophils.ConclusionsCirculating ACLF-patient neutrophils exhibit alterations in number, phenotype, gene expression and function, which was associated with poor outcome and shaped by the ACLF circulatory environment. Inhibiting glycolysis can reverse neutrophil dysfunction in ACLF patients.
Multilayer Maraging/CoCrNi Composites With Synergistic Strengthening-Toughening Behavior
Authors: Chen C. X. , Ge Y. F. , Fang W. , Zhang X. , Liu B. X. , Feng J. H. , Yin F. X. 
Published: February 2021

A novel multilayer maraging/CoCrNi composite with good mechanical properties was successfully fabricated by a vacuum hot-rolling and aging treatment. The yield strength, tensile strength, uniform elongation, and fracture elongation reached 1,151, 1,380 MPa, 15.7, and 24% respectively, realizing the aim of synergistic strengthening–toughening by effectively improving the yield strength of the CoCrNi alloy and strain-hardening capacity of the maraging steel. The vacuum state, high rolling reduction ratio, and alloy element diffusion are beneficial in strengthening the clad interface. The good work-hardening capacity of the CoCrNi alloy compensates for the poor strain-softening behavior of the maraging steel, effectively delaying the premature localized necking of the multilayer composites. The strengthening–toughening mechanism of the multilayer maraging/CoCrNi composites is mainly attributed to the strong interface, nanoscale precipitation, and strain-induced twinning.
Lung Metastases in Newly Diagnosed Esophageal Cancer: A Population-Based Study
Authors: Guo Jida , Zhang Shengqiang , Li Huawei , Hassan Mohamed Osman Omar , Lu Tong , Zhao Jiaying , Zhang Linyou 
Published: February 2021

BackgroundEsophageal cancer is one of the most common cancer types, with its most common distant metastatic site being the lung. Currently, population-based data regarding the proportion and prognosis of patients with esophageal cancer with lung metastases (ECLM) at the time of diagnosis is insufficient. Therefore, we aimed to determine the proportion of patients with ECLM at diagnosis, as well as to investigate the prognostic parameters of ECLM.MethodsThis population-based observational study obtained data from the Surveillance, Epidemiology, and End Results (SEER) database registered between 2010 and 2016. Multivariable logistic regression was performed to identify predictors of the presence of ECLM at diagnosis. Multivariable Cox regression and competing risk analysis were used to assess prognostic factors in patients with ECLM. Median survival was estimated using Kaplan–Meier curves.ResultsOf 10,965 patients diagnosed with esophageal cancer between 2010 and 2016, 713 (6.50%) presented with initial lung metastasis at diagnosis. Lung metastasis represented 27.15% of all cases with metastatic disease to any distant site. Considering all patients with esophageal cancer, multivariable logistic regression indicated that pathology grade, pathology type, T staging, N staging, race, and number of extrapulmonary metastatic sites were predictive factors for the occurrence of lung metastases at diagnosis. The median survival time of patients with ECLM was 4.0 months. Patients receiving chemotherapy or chemoradiotherapy had the longest median overall survival, 7.0 months. Multivariable Cox regression indicated that age, histology type, T2 staging, number of extrapulmonary metastatic sites, and treatment (chemotherapy, radiotherapy, or chemoradiotherapy) were independent predictors for overall survival (OS). Multivariable competing risk analysis determined that age, number of extrapulmonary metastatic sites, and treatment were independent predictors for esophageal cancer-specific survival (CSS).ConclusionThe findings of this study may provide important information for the early diagnosis of ECLM, as well as aid physicians in choosing appropriate treatment regimens for these patients.
Evaluation of the Therapeutic Effect of Adjuvant Transcatheter Arterial Chemoembolization Based on Ki67 After Hepatocellular Carcinoma Surgery
Authors: Zhao Yu-Fei , Xiong Xiu , Chen Kai , Tang Wei , Yang Xu , Shi Zheng-Rong 
Published: February 2021

Background and aimsThis study aimed to determine the relationship between Ki67 expression and the efficacy of postoperative adjuvant transcatheter arterial chemoembolization (PA-TACE) in patients with hepatocellular carcinoma.MethodsThe Kaplan-Meier method was used to analyze the recurrence-free survival (RFS) and overall survival (OS) rates between the sub-groups in the ki67 low expression group and the ki67 high expression group and analyze the relationship between the expression of Ki67 and the efficacy of TACE.ResultsAfter PSM, there was no significant difference in the RFS and OS between the surgery + TACE and surgery subgroups after 1, 2, or 3 years (RFS: 63.9%, 55.6%, and 42.9% vs. 83.3%, 63.9%, and 55.6%, respectively, P = 0.279; OS: 91.7%, 83.3%, and 74.3% vs. 91.7%, 88.9%, and 71.4%, respectively, P = 0.890) in the Ki67 low-expression group. The RFS and OS were higher in the surgery + TACE subgroup than the surgery subgroup after 1, 2, and 3 years (RFS: 80.0%, 77.5%, and 69.2% vs. 53.5%, 39.5%, and 32.6%, respectively, P&lt;0.001; OS: 97.5%, 85.0%, and 79.5% vs. 79.1%, 48.8%, and 42.9%, respectively, P = 0.001) in the Ki67 high expression group. The RFS was higher in the Ki67 high-expression subgroup than the low-expression subgroup after 1, 2, and 3 years, and OS had no significant difference (RFS: 80.0%, 79.5%, and 69.2% vs. 67.4%, 56.5%, and 46.7%, respectively, P = 0.035; OS: 97.5%, 85.0%, and 79.5% vs. 93.5%, 82.6%, and 75.6%, respectively, P = 0.665) in the surgery + TACE group.ConclusionsFor patients with hepatocellular carcinoma and high expression of Ki67 (Ki67≥20%), adjuvant hepatic artery chemoembolization after radical liver tumor resection effectively reduced the probability of tumor recurrence after surgery and prolonged the OS of patients. High Ki67 expression during the post-operative follow-up evaluation of hepatocellular carcinoma patients is an indicator for adjuvant TACE therapy.
Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma
Authors: Yang Changhong , Chen Jialei , Yu Zhe , Luo Jing , Li Xuemei , Zhou Baoyong , Jiang Ning 
Published: February 2021

Gallbladder carcinoma (GBC), which has high invasion and metastasis risks, remains the most common biliary tract malignancy. Surgical resection for GBC is the only effective treatment, but most patients miss the opportunity for curative surgery because of a lack of timely diagnosis. The aim of this study was to identify and verify early candidate diagnostic and prognostic RNA methylation related genes for GBC via integrated transcriptome bioinformatics analysis. Lists of GBC-related genes and methylation-related genes were collected from public databases to screen differentially expressed genes (DEGs) by using the limma package and the RobustRankAggreg (RRA) package. The core genes were collected with batch effects corrected by the RRA algorithm through protein interaction network analysis, signaling pathway enrichment analysis and gene ranking. Four modules obtained from four public microarray datasets were found to be related to GBC, and FGA, F2, HAO1, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CTH, EPHX2, HSD17B6, AKR1C4, CFHR3, ENNP1, and NAT2 were revealed to be potential hub genes involved in methylation-related pathways and bile metabolism-related pathways. Among these, FGA, CFH, F2, HPX, and PIPOX were predicted to be methylated genes in GBC, but POPIX had no modification sites for RNA methylation. Furthermore, survival analysis of TCGA (the Cancer Genome Atlas) database showed that six genes among the hub genes, FGA, CFH, ENPP1, CFHR3, ITIH4, and NAT2, were highly expressed and significantly correlated with worse prognosis. Gene correlation analysis revealed that the FGA was positively correlated with the ENPP1, NAT2, and CFHR3, while CFH was positively correlated with the NAT2, CFHR3, and FGA. In addition, the results of immunohistochemistry (IHC) showed that the expressions of FGA, F2, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CFHR3, NAT2, and ENPP1 were higher in GBC tissues than that in control tissues. In conclusion, two genes, FGA and CFH, were identified as RNA methylation-related genes also involved in bile metabolism in GBC, which may be novel biomarkers to early diagnose and evaluate prognosis for GBC.