bluuebunny/crossref_metadata_2025_split huggingface dataset:
UJI AKTIVITAS ANTIINFLAMASI INFUSA BUNGA PUKUL EMPAT SECARA IN VITRO
Authors: Ihsan Hidayatul , Pratama Iman Surya , Hanifa Nisa Isnaeni 
Published: July 2021

The stew of Mirabilis jalapa L. flower has been used traditionally as an anti-inflammatory. Phytochemical screening of Mirabilis jalapa L. flower showed flavonoid content which is known to have anti-inflammatory activity. This study aims to determine the anti-inflammatory activity of Mirabilis jalapa L. flower infusion using the red blood cell membrane stabilization method by inducing hypotonicity and heat. The test group consisted of Mirabilis jalapa L. flower infusion with a concentration of 2.5%; 5%; and 10% w / v, positive control (diclofenac sodium 0.01% w / v and aspirin 0.01% w / v), and negative control (distilled water). The anti-inflammatory activity of Mirabilis jalapa L. flower infusion was determined by the percentage of membrane stabilization and inhibition of red blood cell hemolysis. Data from the test results were statistically analyzed both parametric and nonparametric with SPSS. Mirabilis jalapa L. flower infusion contains flavonoids, tannins and terpenoids. The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% ( p≤0.05). From these results, it can be concluded that the Mirabilis jalapa L. flower infusion has potential as an anti-inflammatory by stabilizing the red blood cell membrane.
Systems Nutrology of Persons with Tuberculosis Identifies Specific Dietary Profiles Associated with Dysglycemia
Authors: Arriaga María B. , Araújo-Pereira Mariana , Andrade Vanessa M. B. , Fernandes Catarina D. , Sales Caio , Aliaga Juan , Shivakoti Rupak , Lecca Leonid , Calderon Roger I. , Andrade Bruno B. 
Published: July 2021

Abstract
Objective: Dietary pattern may be associated with overall nutritional status that can alter the risk of tuberculosis (TB) disease. This complex interaction can be further intricated by additional metabolic comorbidities such as dysglycemia (diabetes or prediabetes). This study aimed at identifying dietary pattern associated with dysglycemia in TB patients. Research Methods & Procedures: A prospective cohort study of TB patients and their household contacts was conducted between February and November 2017 in Lima, Peru. Among the 269 patients, 85 were considered healthy, 48 had dysglycemia, 75 had TB and 61 had TB-dysglycemia. Food intake was collected using a food frequency questionnaire and a Systems Nutrology analytical approach was employed to identify dietary pattern associated with these groups. Potential associations between clinical factors and dietary pattern were also analyzed.Results: Three dietary patterns were identified based on the food intake profile of the study participants. Normoglycemic TB patients more often had the dietary pattern 1, while healthy individuals more frequently presented with the dietary pattern 2 and persons with TB-dysglycemia were more represented in the dietary pattern 3. Conclusion: TB-dysglycemia was mainly associated with the increased intakes of rice and cereals, fast food and oils. The identification of distinct dietary patterns involved with TB and dysglycemia may help to guide nutritional interventions to optimize patient care.
Clustering and Graph Mining Techniques for Classification of Complex Structural Variations in Cancer Genomes
Authors: Gómez-Sánchez Gonzalo , Delgado-Serrano Luisa , Carrera David , Torrents David , Berral Josep 
Published: July 2021

Abstract
For many years, a major question in cancer genomics has been the identification of those variations that can have a functional role in cancer, and distinguish from the majority of genomic changes that have no functional consequences. This is particularly challenging when considering complex chromosomal rearrangements, often composed of multiple DNA breaks, resulting in difficulties in classifying and interpreting them functionally. Despite recent efforts towards classifying structural variants (SVs), more robust statistical frames are needed to better classify these variants and isolate those that derive from specific molecular mechanisms. We present a new statistical approach to analyze SVs patterns from 2,392 tumor samples from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium and identify significant recurrence, which can inform relevant mechanisms involved in the biology of tumors. The method is based on recursive KDE clustering of 152,926 SVs, randomization methods, graph mining techniques and statistical measures. The proposed methodology was able not only to identify complex patterns across different cancer types but also to prove them as not random occurrences. Furthermore, a new class of pattern that was not previously described has been identified.
The role of long noncoding RNA AL161431.1 in the development and progression of pancreatic cancer
Authors: Ma Gang , Li Guichen , Fan Wufeng , Xu Yuanhong , Song Shaowei , Guo Kejian , Liu Zhe 
Published: July 2021

Abstract
Pancreatic cancer is known for its notorious fast progression and poor prognosis. Long noncoding RNA (lncRNA) AL161431.1 has been reported to be involved in the pathogenesis of different cancers. In this study, we explored the role of lncRNA AL161431.1 in the development and progression of pancreatic cancer by bioinformatic analysis, in vitro and in vivo experiments in pancreatic cancer BxPC-3 and SW1990 cells, as well as clinical samples. We found that lncRNA AL161431.1 was highly expressed in pancreatic cancer cells and tissues. Knock down of lncRNA AL161431.1 led to increased cancer cell death and cell cycle arrest. Xenograft growth of SW1990 cells with stable knockdown of lncRNA AL161431.1 in mice was significantly slower than that of SW1990 cells with scrambled control shRNA. Finally, we showed the involvement of lncRNA AL161431.1 in pancreatic cancer was related to its promotion of epithelial mesenchymal transition process.
Proteins in cord blood as a new target for prevention and treatment of bronchopulmonary dysplasia: a proteomic analysis
Authors: Wang Juan , Mao Jianping , Chen Gang , Huang Yuanmei , Zhou Jinjin , Gao Changlong , Sun Jun 
Published: July 2021

Abstract
This study aimed to explore the proteins in cord blood that could regulate the development of neonatal bronchi and lungs, and to find a new target for the prevention and treatment of bronchopulmonary dysplasia (BPD). In this study, proteomic analysis was used to analyze the proteins in cord blood of preterm and term infants. A total of 100 differentially expressed (57 up-regulated and 43 down-regulated) proteins were identified from preterm with BPD and term infants cord blood (fold change ≥ 1.5, P value < 0.05). GO analysis revealed that the major enrichment functions of these differential proteins were multi-organism process, stimulus, immune system process growth, reproductive process, development process and antioxidant activity. The signaling pathways involved included insulin resistance, insulin signaling pathway, IL−17 signaling pathway, PI3K−Akt signaling pathway, NF−kappa B signaling pathway, glucagon signaling pathway, apoptosis, MAPK signaling pathway, as well as glycolysis/gluconeogenesis. Further protein and protein interact (PPI) analysis revealed that the interacting proteins involved were PGAM1, CNN2, HSP90AA and DAG1, which were all crucial in the development of BPD. We found that these differential proteins in cord blood might regulate bronchopulmonary development through the abovementioned signaling pathways or their interaction proteins, which may provide a new research direction for the intervention of BPD.
Intron-Containing Hairpin RNA Interference Vector for OBP8 Show Promising Mortality in Peach Potato Aphid
Authors: Afroz Amber , Aslam Safeena , Rashid Umer , Malik Muhammad Faheem , Zeeshan Nadia , Khan Muhammad Ramzan , But Muhammad Qasim Shahzad , Khan Sabaz Ali 
Published: July 2021

Abstract
Myzus persicae is a devastating pest affecting potato production. RNA Interference technology is used against essential odorant binding protein 8 (OBP8) to enhance protection against Myzus persicae in potato. Gene was isolated, sequenced and GenBank IDs were allotted and ERNAi was used to design siRNA targets from OBP8 with no off-targets. Multiple Sequence Alignment show M. persicae OBP8 resemblance with Acyrthosiphon pisum, Rhopalosiphum maidis, Aphis fabae, and Sitobion avenae. DsRNA (7 µg/µl) oral acquisition had resulted in 69% mortality and 58% reduction in OBP8 expression 8D post dsRNA feeding in comparison to control. Golden Gate (GG) cloning based RNAigg is used for RNA interference taking advantage of type IIs restriction enzyme Eco31I. Agro infiltration assay was used for introduction of intron-containing hairpin RNA (ihpRNA) in Solanum tuberosum. Aphids feeding on transgenic S. tuberosum show 57.6% mortality and 49% reduction in OBP8 expression 8d post-feeding in comparison to control. This work proves OBP8 as promising ihpRNA targets in potato and related crops for whom Myzus is a devastating target.
Revealing Silver Effects on Bacterial Cell Structure by Atomic Force and Scanning Electron Microscopies
Authors: Tambosi Reem , Djafari Jamila , Liotenberg Sylviane , Ouchane Soufian , Kebaili Nouari 
Published: July 2021

Abstract
The use of silver (Ag + ) as an antimicrobial under different forms and at different scales, appears in numerous applications such as in health care, food industry, clothing, fabrics and disinfectants. Yet, there is still important gaps regarding the complete comprehension of the mechanisms of its actions on bacteria. In a previous work, we demonstrated that, silver and copper severly damage membrane proteins involved in photosynthesis and respiration in bacteria exposed to metal excess. Here, we are presenting complementary data using AFM and SEM microscopies, that reveals (i) the drastic effects of Ag + ions on the morphology and structure of cell membrane and (ii) the formation of Ag + aggregates that adhere to the bacterial cell surface in Rubrivivax (R.) gelatinosus . Impacts of Ag + ions on R. gelatinosus are compared to those on the most commonly studied bacteria (Escherichia (E.) coli and Bacillus (B.) subtilis ), while considering the effect of culture grown media on the modification of silver ions. Altogether, these results reveal other levels and subtle aspects of Ag + toxicity to be taken into account in understanding the general mechanisms of metal toxicity in bacteria.
Ex Vivo Microsphiltration Profile of Plasmodium Falciparum Infected Red Blood Cells in Patients with Malaria in Kéniéroba, Mali: Exploring the Spleen in Vivo Retention Function
Authors: KEITA Bourama , Diakité Seidina , Guindo Agnes , Konaté Drissa , Traoré Karim , Sangaré Modibo , Diawara Sory , Sanogo Ibrahim , Diarra Bakaina , Diakité Mahamadou 
Published: July 2021

Abstract
Malaria pathophysiology is not still fully understood. The main mechanisms of malaria involve the synergistic interactions between host and parasite. Although, the role of the spleen has been mentioned in various clinical forms of malaria, a supportive clinical evidence is still needed. We conducted a pilot study to determine the impact of the spleen functional state in different clinical forms of malaria.
Ex vivo microsphiltration was used to assess the splenic function in patients received during routine consultation with mild malaria at the Kéniéroba health center, a ​​high malaria endemic area in Mali.
A total of 25 patients were enrolled for ex vivo microsphiltration. Spleen was non-palpable (Hackett stage 0) in two patients, palpable with deep inspiration (Hackett stage 1) in 22 patients and without deep inspiration (Hackett stage 2) in one patient, parasitaemia ranged from 5360 trophozoites/µl to 342720 trophozoites/µl with a mean parasitemia of 50774 trophozoites/µl ± 65540 trophozoites/µl. The mean hemoglobin level was 11.2g/dl ± 1.2 [8.7-13.4]. The retention rate of the infected red blood cell ranged from 11.11% to 94.44% with 65.4% ± 23.7% on average. A higher ex vivo retention rate of infected red blood cells was observed in patients with Hackett stage other than 0 (p= 0.03). This pilot study proved that it was feasible to use the ex vivo microsphiltration to explore the spleen filtering function in malaria patients.
Initial experience with the combination of atezolizumab and bevacizumab in patients with unresectable hepatocellular carcinoma progressing after tyrosine kinase inhibitor therapy: A multicenter prospective observational study
Authors: Sugimoto Rie , Satoh Takeaki , Ueda Akihiro , Senju Takeshi , Tanaka Yuki , Yamashita Shinsaku , Koyanagi Toshimasa , Kurashige Tomoyuki , Higuchi Nobito , Nakamura Tsukasa , Tanaka Masatake , Ooho Aritsune , Uchimura Koutarou , Kuniyoshi Masami , Tada Seiya , Aratake Yoshifusa , Motomura Kenta , Kohjima Motoyuki 
Published: July 2021

Abstract
Background
There is no evidence for the efficacy of atezolizumab plus bevacizumab treatment in patients with hepatocellular carcinoma previously treated with tyrosine kinase inhibitors (TKIs).
Methods
Twelve patients treated with atezolizumab plus bevacizumab for unresectable hepatocellular carcinoma (HCC) and previously treated with TKIs were enrolled in the study.
Results
The treatment lines ranged from second line to sixth line. HCC staging was Barcelona Clinic Liver Cancer (BCLC) stage B in four cases and BCLC stage C in eight cases. The overall response rate and disease control rate according to the response evaluation criteria in solid tumors (RECIST) were 18.1% and 54.4%, respectively. Progression-free survival was 2.7 months, indicating that early response to treatment may differ depending on the type of previous therapy. The side effects profile also differed from that observed in IMbrave150, a phase 3 trial of atezolizumab plus bevacizumab, with many adverse events related to liver reserve.
Conclusions
The therapeutic effects and side effects differed from those previously reported during the treatment course of atezolizumab plus bevacizumab as first-line therapy.
Exploring The New Rehabilitation Model of Hearing Impaired Children from the Perspective of Family
Authors: Yu Mengxiao , Xie Haixia , Chen Qiwen , Yao Shengnan 
Published: July 2021

Abstract
The primary aim of the study was to obtain the views of parents on the role of family efficacy in children's rehabilitation stage through investigation. The secondary objective was to evaluate the important role of family factors in the rehabilitation of hearing-impaired children. The third objective was to explore the willingness of parents to accept online courses, parents' Union and personalized family therapy.This study included 480 parents of hearing-impaired children. The questionnaire was distributed and collected in December 2020. And the parents of hearing-impaired children in the stage of postoperative rehabilitation were selected as the research objects.Although the rehabilitation process of hearing-impaired children is affected by many factors, family factors play a dominant role. In the rehabilitation process of hearing-impaired children, parents need to spend a lot of money, time and energy. At the same time, for the effect of rehabilitation, most parents tend to ignore the mental health rehabilitation of hearing-impaired children, only pay attention to the rehabilitation of speech clarity, hearing ability, communication ability of children. Moreover, parents generally believe that the function of the family is mainly responsible for the children's daily life, and only a few families are responsible for the language rehabilitation and mental health of hearing-impaired children. Therefore, parents' understanding of family efficacy is not comprehensive enough. And through the survey, we can see that from the perspective of parents, parents' lack of professional knowledge, lack of self-confidence and time factors will affect children's rehabilitation process. For the measures we proposed, such as online courses, parents' Union, personalized family therapy mode, parents of hearing-impaired children generally have a high acceptance. It can be seen that these measures can promote family efficacy and improve the rehabilitation effect of hearing-impaired children to a certain extent.The rehabilitation of hearing-impaired children needs to extend from schools and rehabilitation institutions to families to form a more unified and comprehensive rehabilitation system,especially on children's psychological rehabilitation. The corresponding models and Countermeasures of hearing-impaired children's family participation in China are extremely insufficient.Based on this, this study puts forward hypothetical Countermeasures in the form of questionnaire, investigates the recognition of parents, explores the innovation of family participation mode of hearing-impaired children, and puts forward accurate countermeasures.
EEM-MAC: Enhanced Energy Efficient Mobility aware MAC protocol for Mobile Internet of Things
Authors: VC Diniesh , G Murugesan 
Published: July 2021

Abstract
Mobile wireless sensor networks (MWSNs) have become a foremost solution in many emerging applications both in industry and academia. Moreover, considering the mobile node in WSN is a challenging task to designing efficient communication protocols, specifically at a medium access control (MAC) layer. Most of the existing protocols consider only for static and slow mobility. To meet with future MIoT applications, in this paper we propose Enhanced Energy Efficient Mobility aware MAC (EMM-MAC) protocol. Our EMM-MAC protocol consists of 3 contributions i) static synchronization and mobility handling phase to support both environments, ii) By using queue length-based channel access priority for static nodes, and iii) the combined highest signal strength and node status-based channel access priority for mobile nodes without any control packet overhead. The simulation results verify that EMM-MAC yields a notable improvement in the average power consumption, packet latency, and packet delivery ratio performances against the well-known mobility-aware MAC protocols under different mobility models and environments.
Assessment of the Impact of COVID-19 Pandemic on The Education and Psychosocial Wellbeing of School-Going Children in Bududa District, Uganda
Authors: Batte Charles , Semulimi Andrew Weil , Mutebi Ronald Kasoma , Mukisa John , Olum Ronald , Bongomin Felix 
Published: July 2021

Abstract
Background: The coronavirus disease – 2019 (COVID-19) pandemic has caused devastating effects across all sectors. The closure of schools negatively affected school going children globally. The present study sought to assess the effect of the ongoing COVID-19 pandemic and measures put in place to curb its spread on the psychosocial wellbeing of school going children in Bududa District, in Uganda. Methods: This was a cross-sectional study in which randomly enrolled school-going children from three conveniently selected sub-counties, Bukalasi, Bushiyi, and Bushika, in Bududa District, Uganda. Pretested standardised semi-structured questionnaires were used to collect data on the impact of COVID-19 pandemic on education and psychosocial status of the participants. Results: A total of 210 participants were studied. Of this, 122 (53.3%) were male, 203 (96.7%) lived in rural settings, and 155 (73.8%) were staying with both parents. The median age of the participants was 12 (interquartile range: 9 -15) years. One hundred ninety one (90.9%) participants were in primary school. Overall, 154 (73.3%) were worried about not going back to school during the lock down, however, 109 (51.9%) were able to return to school after the easing of the lockdown. Eighty-five (40.5%) participants received reading material at home during lockdown with 64 (30.5%) of the participants accessing teachings through radio. Of the 210 participants, 174 (82.9%) felt worried about the future, 172 (81.9%) felt like they were going to die during lockdown, 141 (67.1%) felt lonely at times, and 129 (61.4%) felt sad or low sometimes during the COVID-19 pandemic. A quarter (54) of 210 participants faced abuse of which 42 (77.8%) faced physical abuse from parents. Of 31 participants who sought help, 19 (61.3%) went to their parents with none getting help from non-governmental organisations. Fifty (23.8%) participants were engaged in work; 33 (66%) were paid, 4 (8%) were forced to work, and 22 (44%) faced physical challenges at their jobs.Conclusion: There was a substantial impact of the COVID-19 pandemic on the education and psychosocial wellbeing of school-going children in Bududa District. A comprehensive child psychological support service should be scaled up to provide support to school going children in vulnerable communities.
Agroforestry And Reforestation With The Gold Standard - Decision Analysis of A Voluntary Carbon Offset Label
Authors: Netter Leonie , Luedeling Eike , Whitney Cory 
Published: July 2021

Abstract
Voluntary standards help to ensure the quality of projects eligible for carbon offsetting, i.e. selling carbon certificates. However, in deciding on whether to adopt such standards the managers of carbon offset projects are faced with uncertainty regarding the costs and risks involved. Decision Analysis provides a helpful set of tools that can support such decisions by forecasting outcomes under different scenarios. We applied Decision Analysis methods to generate models for the decision to certify two projects in Costa Rica with the voluntary carbon offset label Gold Standard. We evaluated certifying an additional site of a partially certified reforestation project, as well as the initial certification of an agroforestry project. We calibrated and interviewed decision-makers and stakeholders of the certification projects to identify important parameters and translated these into a decision model. We ran the final decision model as a Monte Carlo simulation to project plausible ranges of decision outcomes, expressed as Net Present Values and annual cash flows. We identified critical uncertainties and research priorities by using the Expected Value of Perfect Information. The results indicate that certification of the two projects would result in a positive Net Present Value. The partially low return on investment of the certification, however, shows the need for projects to undergo thorough evaluation and generate customized strategies before participating in a voluntary carbon offset scheme. The Decision Analysis approaches we describe can help to improve the process of decision making under uncertainty and should be widely adopted for evaluating the potential impacts of certification.
Insights Into The Origin of Selectivity For [2+2] Cycloaddition Step Reaction Involved In The Mechanism of Enantioselective Reduction of Ketones With Borane Catalyzed By A B-Methoxy Oxazaborolidine Catalyst Derived From (–)-β-Pinene. A DFT And Combined Topological ELF, NCI And QTAIM Study
Authors: Kettouche Hichem Sadrik 
Published: July 2021

Abstract
Theoretical studies on [2+2] cycloaddition step involved in the Enantioselective Reduction of ketones with borane catalyzed by a B-MethoxyOxazaborolidine Catalyst Derived from Pinene has been performed by means of the Density Functional Theory method (DFT) at MPWB1K /6-31G (d,p). The formation of the M5a(S) complexes via transition state TSa(S) was the more favorable pathway among other [2+2]cycloaddition competing steps. The explanation of the formation of O-B and N-B through two-stage one-step mechanism was allowed by means of the electron localization function (ELF) topological analysis. NCI and QTAIM analysis of the two computed transition states TSa(S) and TSa(R) indicate that the difference between both in term of stability comes mainly from the orientation of the methanediyl group inside the pinene skeleton, which implies that CH-H…O interaction found at TSa(S) is the great factor that makes it more stable than TSa(R).