bluuebunny/crossref_metadata_2025_split huggingface dataset:
940-P: Prediction of Neurocognitive Disability with Magnitudes of Insulin Resistance and Glucagon-Like Peptide-1 Secretion in Offspring from Mother with Diabetes
Authors: IWASAKI HAJIME , SASAKI JUNKO , SUGAI KEIJI , ITO MARIKO , ISHII KEITARO , AIERKEN MAIERHABA , LI GUOJIAO , SUWANAI HIROTSUGU , TORII MASAAKI , HASHIMOTO-TORII KAZUE , ODAWARA MASATO , SUZUKI RYO 
Published: June 2021

Diabetes during pregnancy has been linked with an increased risk of developing metabolic disorders and behavioral abnormalities in the offspring. However, the correlation between those two phenotypes in offspring from mother with diabetes (OMD) has not been tested. We therefore examined the correlation between motor skill learning and glucose tolerance, including glucagon-like peptide-1 (GLP-1) secretion in OMD. ICR female mice received intraperitoneal injection (i.p.) of 150mg/kg streptozotocin at six weeks old. Control female was administered with the vehicle. Those animals showed over 250mg/dL glucose level at 14 days post injection. Mating was done with normal male and all of pups were born in term. Motor skill learning was tested on accelerated rotarod at postnatal 30 days (P30). A learning index was calculated for each animal by averaging the difference in terminal speed when the mouse fall from the rotor rod. Motor learning is impaired in OMD (P =0.0035). Following the behavior test, insulin tolerance test, oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed. Although the weight of OMD is similar to that of control animals at P21, it was significantly increased after P60 (P &lt;0.0001). The glucose level in the plasma of mice is significantly increased in IPGTT (15, 30min post glucose i.p. injection, p &lt;0.0001) at P60. OMD show higher homeostasis model assessment of insulin resistance (OMD 0.93 vs. control 0.84), and reduced secretion of active GLP-1 (aGLP-1) (OMD 6.70 ng/mL vs. control 8.02 ng /mL) in OGTT. Notably, poor learners of motor skill show significantly lower aGLP-1 secretion than the good learners (P =0.0360). Furthermore, in OMD, neuronal morphology was abnormal in the motor cortex that controls the motor skill learning. These results demonstrate that insulin resistance and the aGLP-1 secretion serve as predictors of motor skill learning deficiencies in OMD.

Disclosure
H. Iwasaki: None. K. Hashimoto-torii: None. M. Odawara: Speaker’s Bureau; Self; Astellas Pharma Inc., Daiichi Sankyo Company, Limited, Eli Lilly Japan K. K., Kyowa Kirin Co., Ltd., MSD Corporation, Novartis AG, Ono Pharmaceutical Co., Ltd., Sumitomo Dainippon Pharma Co., Ltd., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Company Limited. R. Suzuki: Research Support; Self; Mitsubishi Tanabe Pharma Corporation, Nippon Boehringer Ingelheim Co. Ltd., Ono Pharmaceutical Co., Ltd., Sumitomo Dainippon Pharma Co., Ltd., Speaker’s Bureau; Self; MSD K. K., Novartis Pharma K. K., Novo Nordisk Pharma Ltd., Sanofi K. K., Taisho Pharmaceutical Co., Ltd., Takeda Pharmaceutical Company Limited. J. Sasaki: None. K. Sugai: None. M. Ito: None. K. Ishii: None. M. Aierken: None. G. Li: None. H. Suwanai: None. M. Torii: None.
956-P: A Longitudinal Study of Plasma Glycated Albumin in Pregnancy: Findings from a Multiracial U.S. Pregnancy Cohort
Authors: ZHANG CUILIN , WU JING , HINKLE STEFANIE , SACKS DAVID B. 
Published: June 2021

Background: Glycated albumin (GA) represents a useful tool reflecting glycemic homeostasis over a period of 3 weeks and has been recently proposed as a screening marker for diabetes among non-pregnant individuals. However, data on GA during pregnancy are sparse in general and lacking among women of diverse race/ethnicity. We aimed to investigate longitudinal levels of GA in pregnancy among U.S. women of multi-race/ethnicity.
Methods: We quantified GA and cardiometabolic biomarkers using blood samples collected at 8-13, 15-26 (fasting sample), 26-31, and 33-39 gestational weeks from 214 pregnant women without gestational diabetes in the NICHD Fetal Growth Studies-Singletons. We examined distributions of GA across pregnancy and their associations with major characteristics of study participants such as race/ethnicity, pre-pregnancy body mass index (BMI), fetal sex, and selected cardiometabolic biomarkers.
Results: Overall, GA tended to increase from early pregnancy to 2nd trimester and decrease through the 3rd trimester; medians (inter-quartile range) were 12.1% (10.6-13.4), 12.5 (10.7-13.8),12.4 (10.9-13.5) and 11.5 (10.4-12.5) at 8-13, 15-26, 26-31, and 33-39 gestational weeks, respectively. A similar pattern was observed across race/ethnicity groups (p for interaction =0.71). At 8-13 gestational weeks, GA concentrations were significantly and inversely related to pre-pregnancy BMI, waist circumference and waist/hip ratio, and plasma HDL and adiponectin levels, but significantly and positively related to triglyceride levels. Interestingly, GA levels were neither significantly related to HbA1c over pregnancy, nor to fasting inulin, glucose, or HOMA-IR. GA levels did not vary significantly by race/ethnicity or fetus sex.
Conclusion: GA appeared not a sensitive marker for glucose metabolism in pregnancy, although it was significantly related to pre-pregnancy adiposity measures and some cardiometabolic biomarkers during pregnancy.

Disclosure
C. Zhang: None. J. Wu: None. S. Hinkle: None. D. B. Sacks: Research Support; Self; Sebia, Trinity Biotech plc.
976-P: Cardiovascular Risk Predicts Severe Respiratory Failure in Patients Hospitalized with COVID-19
Authors: LOCKHART SAM , SHEIKH ANUM , GRIFFITHS HARY , LATORRE JULIA CALVO , DALY AIDEEN B. , SIBAL LATIKA 
Published: June 2021

Cardiometabolic disease is associated with mortality and requirement for ventilation in COVID-19. Symptomatic atherosclerotic cardiovascular disease (CVD) implies the presence of significant endothelial dysfunction and is a risk factor for severe COVID-19. However, many patients with no history of CVD exhibit severe COVID-19. We hypothesised that CVD risk calculators could serve as a proxy for significant endothelial dysfunction and predict COVID-19 outcomes in those without symptomatic CVD.
We reviewed 230 patients aged 35 to 65 years, hospitalized with COVID-19 at our institution. 190 patients with no history of symptomatic CVD were included. 23% developed severe respiratory failure (RF, requiring mechanical or non-invasive ventilation), while 4% (7/164) of those without prior end-stage renal disease required renal replacement therapy (RRT).
In univariate analysis, age (β = 0.057 ± 0.02, p= 0.02), BMI (β = 0.05 ± 0.03, p = 0.049) and T2DM (β = 0.81 ± 0.41, p = 0.048) predicted RF. In keeping with a link between insulin resistance and severe COVID-19, an association was observed between triglycerides and RF (β = 0.26 ± 0.14, P = 0.058). CVD risk indices QRISK3 (0.07 ± 0.02, P &lt;0.001), a CVD Risk Algorithm that predicts the risk of a CV event in next 10 years, and CHADS2VASC (0.53 ± 0.20, P=0.01) were both associated with RF. Age and QRISK3 were associated with a requirement for RRT and length of stay.
We observed a threshold effect for QRISK3: when 10 year risk of a CV event was &gt; 5% the risk of RF markedly increased (OR: 6.86 (95% CI: 2.30 - 20.5, P&lt;0.001). The risk of RF was similar across all strata of QRISK3 &gt;5%. A similar trend was observed when analysis was limited to those with T2DM (Ventilatory support in T2DM: QRISK&lt;5% - 0/8, QRISK&gt;5%: 11/30). All cases of RRT occurred in those with QRISK &gt;5%.
CVD risk calculators predict severe COVID-19, supporting a role for subclinical CVD in severe COVID-19. Patients hospitalized with QRISK3 &gt;5% may be candidates for early intervention and enhanced monitoring.

Disclosure
S. Lockhart: None. A. Sheikh: None. H. Griffiths: None. J. Calvo latorre: None. A. B. Daly: None. L. Sibal: None.

Funding
National Institute for Health Research
CFD Analysis on Francis Turbine to Analyse Erosion Wear Due to Sediment Flow
Authors: Yadav Uttam Singh , Vishwakarma Shravan , Mishra Jitendra 
Published: June 2021

In present work Computational fluid dynamics analysis based erosion wear prediction is performed for Francis turbine components, especially the runner. For the geometrical parameters, Francis turbine model with steady state condition and viscous flow turbulence SST model using ANSYS Fluent. The erosion effect on all the three component such as spiral casing, runner & draft tube has been studied for different concentration of sand particles from 1% - 6%. For each of those concentration the effect of variation in size has been studied for different sizes 10 ?m - 80 ?m. Further the effect of total erosion was also analyzed for different particle size. Erosion damage is found close to the upper and lower portions of the leading edge of the stay vane. some erosion spots at guide vane on the blade pressure side where suction side has minimum erosion. Maximum erosion damage observed on runner especially at the middle of the blade. The draft tube situated closer to runner having highest velocity due to high absolute velocity of water coming out from the runner does not produce any serious erosion effect. Results shows that erosion rate is maximum on runner at particle size 80 ?m for all sand concentration 1% to 6% and minimum at 30 ?m. Thus, 30 ?m is the optimum size of sand particles for the erosion.
Effect of ultraviolet-C light on the environmental bacterial bioburden in various veterinary facilities
Authors: Browne Katrina L. , Crowley James D. , Tan Christopher J. , O'Sullivan Christopher B. , Walsh William R. 
Published: June 2021

Abstract

OBJECTIVE
To determine the effect of a mobile UV-C disinfection device on the environmental bacterial bioburden in veterinary facilities.

SAMPLES
40 swab samples of surfaces from the operating theaters of 3 veterinary hospitals and 1 necropsy laboratory.

PROCEDURES
Various surfaces were swabbed, and collected material was eluted from the swabs in PBSS. Then, an aliquot of the sample fluid was processed with a bacteria-specific rapid metabolic assay to quantify bacterial bioburden. Each site was then treated with UV-C light with an automated disinfection device for approximately 45 minutes. The same surfaces were swabbed following UV-C treatment, and bioburden was quantified. The bioburden at additional time points, including after a second UV-C treatment, was determined for the small animal operating theater.

RESULTS
All surfaces at all sites had a persistent viable bacterial population following manual cleaning. Disinfection with UV-C achieved a mean bioburden reduction of 94% (SD, 5.2%; range, 91% to 95%) for all surfaces, compared with manual disinfection alone. Repeated UV-C treatment of the small animal operating theater reduced mean bioburden by 99% (SD, 0.8%), including no detectable bacteria on 4 of 10 surfaces.

CONCLUSIONS AND CLINICAL RELEVANCE
Disinfection with UV-C light may be a beneficial adjunct method for terminal disinfection of veterinary operating theaters to reduce environmental bioburden.
Attenuating Effect of Vitamin E and Tinospora cordifolia on Bisphenol-A Induced Apoptosis in Goat Testis
Authors: Sharma R. K.  , Gandhi A  
Published: June 2021

During the present study effect of Vitamin E and Tinospora cordifolia on BPA induced goat (Capra hircus) testicular tissue was evaluated. Testicular tissue was exposed to varied concentrations of BPA, Vitamin E and Tinospora cordifolia in three experimental groups along with their specific control for 4 and 8hrs. In group I, the cultured tissue was exposed to three doses of Bisphenol-A viz. 0.01, 1.0 and 100 nM/ml. In group II, Vitamin E (0.1µM/ml) was supplemented along with BPA (0.01, 1.0, 100 nM/ml) and in group III Tinospora cordifolia extract (250 µg/ml) was added with BPA (0.01, 1.0, 100 nM/ml) in the culture media. With the increase in dose level as well as the exposure duration, the frequency of apoptotic cells increased significantly (p< 0.05), as revealed by acridine orange and methylene blue staining. BPA exposure also induced a decline in the antioxidant enzyme activity such as catalase, glutathione peroxidase and reduced glutathione. Vitamin E and Tinospora cordifolia resulted in a decrease in the percentage of apoptotic cells and an increase in levels of catalase, glutathione peroxidase and reduced glutathione activity was observed. Hence, it is concluded that BPA induced more damage at its higher concentration and the antioxidant Vitamin E and Tinospora cordifolia ameliorate the oxidative stress-induced changes, thereby suggesting the potential of Vitamin E and Tinospora cordifolia in counteracting the cellular damage induced by agents like BPA.
Peculiarities of surgical treatment of patients with gastroesophageal reflux disease
Authors: Matviychuk B. O. , Stasyshyn A. R. , Hurayevskyy A. A. 
Published: June 2021

Objective. To analyze the effectiveness of various methods of laparoscopic antireflux operations in patients with gastroesophageal reflux disease.
Materials and methods. The results of treatment of 177 patients with gastroesophageal reflux disease from 2015 to 2021 were analyzed. Patients were divided into three groups: 1st - 62 patients who underwent laparoscopic antireflux surgery of our own modification (Patent of Ukraine No 59772); 2nd - 91 patients who underwent laparoscopic Nissen fundoplication; 3rd - 24 patients who underwent laparoscopic Toupet fundoplication.
Results. 36 months after surgery in the 1st group the recurrence rate of gastroesophageal disease was 1.6%, recurrence of hernia of the esophageal orifice - 1.6%, in the 2nd group - 7.7 and 3.3%, respectively; in the 3rd group - 12.5 and 8.3%, respectively.
Conclusions. The application of a new integrated approach to the surgical treatment of gastroesophageal reflux disease reduces the number of postoperative complications (gas -bloat syndrome - 3 times, dysphagia - 5 times), the recurrence rate of gastroesophageal reflux disease - 5 times and recurrence of esophageal hernia - 2 times, improves the quality of life of patients 36 months after surgery in 2.6 times and increases the timeliness of diagnosis and facilitates the choice of differentiated treatment tactics.
Zbrodnia pomorska 1939 r. w powiecie tczewskim: sprawcy, ofiary, pamięć, odpowiedzialność
Authors: Tomkiewicz Monika 
Published: June 2021

Zbrodnia pomorska 1939 r. była przede wszystkim eksterminacją dziesiątek tysięcy przedstawicieli polskiej inteligencji, rozstrzelanej w ramach „Inteligenzaktion”, ale także rolników, robotników i rzemieślników, zamordowanych przez niemieckich sąsiadów. Po zajęciu powiatu tczewskiego przez wojska niemieckie od 2 września 1939 r. władze okupacyjne zaczęły tworzyć obozy internowania i punkty zbiorcze w więzieniach dla ludności polskiej. Na terenie powiatu tczewskiego miejsca takie znajdowały się w więzieniach sądowych w Tczewie i Gniewie, Szkole Rzemieślniczej w Tczewie, na terenie Fabryki „Arkona” w Tczewie, na terenie zamku w Gniewie oraz w Seminarium Duchownym w Pelplinie. Stamtąd więźniowie wywożeni byli do miejsc straceń w Lesie Szpęgawskim i w koszarach w Tczewie. Egzekucje poprzez rozstrzelanie na terenie koszar prowadzili członkowie załogi obozu „Internierungslager” i komanda SA w Gdańsku. Rozstrzeliwań dokonywano głównie na terenie dawnej prochowni i na placu ćwiczeń za koszarami. Ciała zamordowanych były grzebane na terenie koszar. Zginęło tam wówczas około 120 osób. W grupie tej byli pracownicy polskiej administracji, przedstawiciele inteligencji, adwokacji, nauczyciele i duchowni (w tym 20 księży kapituły pelplińskiej). W wyniku prac ekshumacyjnych przeprowadzonych w 1945, 1947 i 1976 r. zidentyfikowano zwłoki 79 osób. Obóz tczewski funkcjonował do stycznia 1940 r. i w jego murach osadzono około 500 więźniów.
Use Of Anti Hypertension And Factors That Affect The Compliance Of Drinking Anti Hypertension In Hypertension Patients In Cisalak Pasar Depok Health Center
Authors: Bahar Meiskha , Yusmaini Hany , Wahyuningsih Sri 
Published: June 2021

Compliance with treatment is an important factor in the health of hypertensive patients. Compliance is a prerequisite for the effectiveness of hypertension therapy, whereas patient non-compliance with antihypertensive drugs is one of the main factors in the failure of therapy. There are several factors that influence medication adherence and some basic principles of therapy that need to be considered to reach the target of therapy and reduce complications of hypertension.
Hypertension sufferers in the Cisalak Pasar Community Health Center are quite numerous, they are generally elementary school and not schooled (74.2%). Kidney complications and a combination of heart and kidney are also quite large, which is 40%. This study aims to determine the pattern of antihypertensive administration and the relationship of age, education level, degree of hypertension, complications and type of therapy with the level of adherence to taking medication for hypertensive patients at the Cisalak Pasar Community Health Center. The research design used in this study was a descriptive cross-sectional. The subjects of the study were essential hypertension patients at the Cisalak Pasar Health Center, Depok who met the inclusion and exclusion criteria. The results of this study are that the pattern of antihypertensive administration is in accordance with JNC 8, there is no relationship between age, education level, hypertension degree and the number of drugs taken with medication compliance, and there is a relationship between the presence or absence of complications with medication compliance
Tampilan estrus kambing Peranakan Etawa dalam program sinkronisasi menggunakan prostaglandin F2α yang diinjeksikan pada submukosa vulva
Authors: Prastowo Sigit , Yusanti Anna , Nisaa’ Chairul , Sunarto . , Widyastuti Rini 
Published: June 2021

Penelitian ini bertujuan membandingkan tampilan estrus kambing Peranakan Etawa (PE) dalam program sinkronisasi estrus menggunakan hormon PGF2α yang diinjeksikan pada submukosa vulva (SMV). Sebanyak 10 ekor kambing PE berumur 2,5 tahun dan telah mengalami 2 kali partus digunakan sebagai unit percobaan. Injeksi PGF2α secara SMV pada P1 = dosis 0,1578 mg/ml (n=5) dan P2 = dosis 0,0789 mg/ml (n=5) menggunakan dosis ganda dengan interval injeksi 11 hari. Tampilan estrus diamati dan diukur menggunakan Draminski Heat Estrous Detector (DHED) mulai 24 jam setelah injeksi kedua setiap 8 jam hingga tanda estrus menghilang. Nilai hambatan arus listrik (HL) yang ditampilkan oleh DHED selama pengamatan digunakan untuk melihat awal, puncak dan akhir estrus. Hasil pengamatan menunjukkan bahwa nilai HL puncak estrus berbeda dengan awal dan akhir estrus (p<0,05) pada setiap P1 dan P2. Rata-rata nilai HL pada P1 dan P2 masing-masing 480,00±8,16 dan 485,00±12,91. Onset estrus adalah 24 dan 120 jam secara berturut-turut pada P1 dan P2 dan lama estrus adalah 44,00 dan 43,50 jam. Injeksi hormon PGF2α pada kambing PE secara SMW mampu memperlihatkan tampilan estrus dengan onset lebih lama pada dosis hormon yang lebih rendah.
Promising Diagnostic Markers of Colon Cancer
Authors: Volkov S. V. , Lobanov S. L. 
Published: June 2021

Background. The incidence of colon cancer over the past decade has been growing markedly in the Russian Federation, with about 50 % cases detected at stagesIII–IV of the disease, when a clear clinical picture of the disease appears. In this regard, the search for new methods for early diagnosis of RTK is undoubtedly relevant.Objective. To determine the standard composition of the aerobic parietal colon microbiota and the level of cytokines (chemokines and growth factors) in patients with cancer of the left half of the colon and to assess the possibility of using these data in the diagnosis of the tumor process.Materials and methods. Blood tests were performed on the day of the study using two test systems (BioLegend): multiplex kit for determining growth factors, chemokine multiplex kit. The composition of the intestinal microbiota was determined in colon biopsy specimens by the bacteriological method using the standard test systems StaphyTest, StreptoTest, and EnteroTest.Results. There is an increase in the number of Clostridiumspp. and a decrease in Bifidobacteriumspp., E. coli in the colon during the transformation of a healthy person’s mucosa into a malignant tumor (p < 0.05); a clear tendency was revealed for both an increase (EGF, HGF, M-CSF, PDGF-AA, PDGF-BB, IP-10) and a decrease (MCP-1, RANTES) of the level of chemokines and growth factors under colon cancer conditions. In addition to general quantitative changes in the intestinal microbiota, the level of the investigated substances, a statistically significant dependence was established on the sex, age of the patient, as well as the degree of differentiation and form of tumor growth.Conclusion. It was established that changes in the quantitative composition of the intestinal microbiota, the level of some biologically active substances that occur precisely in the conditions of colon cancer, can be interconnected and interdependent, and also serve as an additional diagnostic marker in the detection of a malignant tumor.
Biomarkers of Myopic Choroidal Neovascularization in Women, Determining the Clinical Course and Response to Anti-VEGF Therapy
Authors: Grigorieva A. V. , Iureva T. N. , Kursakova Ju. V. , Ivanova E. I. , Samsonov D. Yu. , Mikhalevich I. M. 
Published: June 2021

Aim: to identify clinical and laboratory biomarkers that determine the nature of the course of myopic choroidal neovascularization (mCNV) and the response to anti-VEGF therapy in women.Material and methods. A prospective non-randomized study was conducted in 52 patients (52 eyes) with active mCNV, treated with ranibizumab intravitreally, 0.5mg. After 12months, the suppression of CNV activity, the number of injections and the fellow eye inclusion in the pathological process were taken into account. There were 2 groups: with a favorable clinical course (n = 31, age – 33,0 ± 5,1 years, anterior-posterior axis (APA) 28,5 ± 0,3 mm) and an unfavorable clinical course (n = 21, age – 34,0 ± 4,1 years, APA – 29,01 ± 0,1 mm). Structural retinal changes, choroid thickness, retinal blood flow, and heart rate were evaluated using OCT and OCTA protocols (Optovue XR Avanti, USA). Studies were conducted before the start of therapy and then one time per month. The concentration of sex and pituitary hormones (ELISA analyzer “Immunohem-2100”), lipoprotein A, Apo B/Apo A (Accent 200 Cormay, Poland), coagulogram data (Helena C-2, UK), and the concentration of highly sensitive C-reactive protein in blood serum before the start of antiangiogenic therapy were studied once.Results. In the first group, 1.4 ± 0.7 ranibizumab injections were administered to suppress the CNV activity. In the opposite group – 3.5 ± 2.1 injections, in 73.7 % of cases, relapses were diagnosed, in 3 cases – primary CNV in the fellow eye. Clinical and laboratory biomarkers of the unfavorable clinical course of mCNV were identified: extreme choroidal thinning, highly organized membranes of a large area, dome-shaped deformation of the posterior pole, excess of the reference values of lipoprotein A, fibrinogen and highly sensitive C-reactive protein by two or more times, an imbalance of sex and pituitary hormones (excess of the reference values of prolactin, follicle stimulating hormone, cortisol, progesterone concentration decrease), a predominant change in the menstrual-ovarian cycle according to the type of amenorrhea and opsomenorrhea.Conclusion. Myopic CNV biomarkers in women allow predicting the response to anti-VEGF therapy, the formation of relapses and the inclusion of the fellow eye in the pathological process.