bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonsequential Response in Mountainous Areas of Southwest China
Authors: Liu Lin , Ye Sheng , Chen Cangyi , Pan Hailong , Ran Qihua 
Published: July 2021

Nonsequential response is the phenomenon where the change of soil water content at the lower layer is larger than that of the upper layer within a set time interval. It is often ignored because of the lack of spatially distributed measurements at the watershed scale, especially in mountainous areas where extensive monitoring network is expensive and difficult to deploy. In this study, the subsurface nonsequential response in a mountainous watershed in Southwest China was investigated by combining field monitoring and numerical simulation. A physics-based numerical model (InHM) was employed to simulate the soil water movement to explore the occurrence of the subsurface nonsequential response. The topographic wetness index [TWI = ln (a/tan b)] was used to distinguish the topographic zone corresponding to the nonsequential response at different depths. The nonsequential response mainly came from the subsurface lateral flow initiated at the soil–bedrock interface or at a relatively impermeable layer. The results showed that the occurrence depth of the nonsequential response increased with precipitation intensity when the time since last event was more than 24 h and the total amount of this event exceeded 37 mm. During a rainfall event, the nonsequential response occurred at the middle layer in the hillslope zone and the deep soil layer beneath the channel. In case of a rainfall event with two peaks, the region observed with nonsequential response expanded. The soil layer at the interface of the bedrock could be saturated quickly, and became saturated upward. This kind of nonsequential response can be observed on the hillslope at the beginning of rainfall events, and then found beneath stream channels afterward. Furthermore, nonsequential response could also happen after rainfall events. The results improved our understanding of nonsequential response and provided a scientific basis for flash flood research in mountainous areas.
An Evaluation of Strong-Motion Parameters at the S-net Ocean-Bottom Seismograph Sites Near the Kanto Basin for Earthquake Early Warning
Authors: Dhakal Yadab P. , Kunugi Takashi 
Published: July 2021

We analyzed strong-motion records at the ground and borehole in and around the Kanto Basin and the seafloor in the Japan Trench area from three nearby offshore earthquakes of similar magnitudes (Mw 5.8–5.9). The seafloor strong-motion records were obtained from S-net, which was established to enhance tsunami and earthquake early warnings after the 2011 great Tohoku-oki earthquake disaster. The borehole records were obtained from MeSO-net, a dense network of seismometers installed at a depth of 20 m in the Tokyo metropolitan area. The ground records were obtained from the K-NET and KiK-net networks, established after the 1995 great Hanshin-Awaji earthquake disaster. The MeSO-net and S-net stations record the shakings continuously, while the K-NET and KiK-net records are based on triggering thresholds. It is crucial to evaluate the properties of strong motions recorded by the S-net for earthquake early warning (EEW). This paper compared the peak ground accelerations (PGAs) and peak ground velocities (PGVs) between the S-net and K-NET/KiK-net stations. Because the MeSO-net records were from the borehole, we compared the PGAs and significant durations of the low-frequency motions (0.1–0.5 Hz) between the S-net and MeSO-net stations from identical record lengths. We found that the horizontal PGAs and PGVs at the S-net sites were similar to or larger than the K-NET/KiK-net sites for the S wave. In contrast, the vertical PGAs and PGVs at the S-net sites were similar to or smaller than those at the K-NET/KiK-net sites for the S wave. Particularly, the PGAs and PGVs for the P-wave parts on the vertical-component records of S-net were, on average, much smaller than those of K-NET/KiK-net records. The difference was more evident in the PGAs. The average ratios of S-wave horizontal to vertical PGAs were about 2.5 and 5 for the land and S-net sites, respectively. The low-frequency PGAs at the S-net sites were similar to or larger than those of the MeSO-net borehole records. The significant durations between the two-networks low-frequency records were generally comparable. Quantification of the results from a larger dataset may contribute to ground-motion prediction for EEW and the design of the offshore facilities.
Framing Contraceptive Use Motivations Among Adolescents and Young Adults Living in Informal Settlements in Kira Municipality, Wakiso District, Uganda
Authors: Mulubwa Chama , Munakampe Margarate Nzala , Namakula Hilda , Hernandez Alison , Ssekamatte Tonny , Atuyambe Lynn M. , Birabwa Catherine , Chemonges Denis , Namatovu Fredinah , Makumbi Fredrick , Tetui Moses 
Published: July 2021

Introduction: The use of contraceptives among adolescents and young adults is one of the most cost-effective strategies to address many sexual and reproductive health (SRH) challenges, including unintended pregnancies, early marriages, and sexually transmitted infections. Despite a high burden of SRH challenges, uptake and unmet needs of modern contraceptives remain low in Uganda, especially among adolescents and young adults in informal settlement settings. This study aimed to explore the motivations of adolescents and young people to use modern contraceptives (or not).Methods: We analysed qualitative data from eight focus group discussions with 88 adolescents and young people aged 18–24 years residing in informal settlements of urban communities in Kira Municipality of Wakiso district, Uganda.Results: Motivations for use (or not) of modern contraceptives were framed by two interrelated constructs, sources of information on contraception and the unacceptable use of contraceptives among adolescents widespread in the community. These two, in turn, formed the scope of knowledge upon which adolescents and young people based their decision on whether or not to access and use modern contraceptives.Conclusion: To be more effective, sexual and reproductive health programs and interventions that aim to motivate the use of modern contraceptives among adolescents and young people in informal settings should be more comprehensive and focused on alleviating individual, health systems, social, religious factors that reinforce negative health-seeking behaviours towards contraceptive use. In addition, there is a need to support adolescents and young people with socio-economic empowering strategies that equip them with sufficient resources to choose contraceptives of their choice.
Comprehensive Transcriptomic Analysis Identifies Novel Antiviral Factors Against Influenza A Virus Infection
Authors: Zhou Ao , Dong Xia , Liu Mengyun , Tang Bin 
Published: July 2021

Influenza A virus (IAV) has a higher genetic variation, leading to the poor efficiency of traditional vaccine and antiviral strategies targeting viral proteins. Therefore, developing broad-spectrum antiviral treatments is particularly important. Host responses to IAV infection provide a promising approach to identify antiviral factors involved in virus infection as potential molecular drug targets. In this study, in order to better illustrate the molecular mechanism of host responses to IAV and develop broad-spectrum antiviral drugs, we systematically analyzed mRNA expression profiles of host genes in a variety of human cells, including transformed and primary epithelial cells infected with different subtypes of IAV by mining 35 microarray datasets from the GEO database. The transcriptomic results showed that IAV infection resulted in the difference in expression of amounts of host genes in all cell types, especially those genes participating in immune defense and antiviral response. In addition, following the criteria of P&lt;0.05 and |logFC|≥1.5, we found that some difference expression genes were overlapped in different cell types under IAV infection via integrative gene network analysis. IFI6, IFIT2, ISG15, HERC5, RSAD2, GBP1, IFIT3, IFITM1, LAMP3, USP18, and CXCL10 might act as key antiviral factors in alveolar basal epithelial cells against IAV infection, while BATF2, CXCL10, IFI44L, IL6, and OAS2 played important roles in airway epithelial cells in response to different subtypes of IAV infection. Additionally, we also revealed that some overlaps (BATF2, IFI44L, IFI44, HERC5, CXCL10, OAS2, IFIT3, USP18, OAS1, IFIT2) were commonly upregulated in human primary epithelial cells infected with high or low pathogenicity IAV. Moreover, there were similar defense responses activated by IAV infection, including the interferon-regulated signaling pathway in different phagocyte types, although the differentially expressed genes in different phagocyte types showed a great difference. Taken together, our findings will help better understand the fundamental patterns of molecular responses induced by highly or lowly pathogenic IAV, and the overlapped genes upregulated by IAV in different cell types may act as early detection markers or broad-spectrum antiviral targets.
Plasma Proteomic Biomarkers Relating to Alzheimer’s Disease: A Meta-Analysis Based on Our Own Studies
Authors: Shi Liu , Buckley Noel J. , Bos Isabelle , Engelborghs Sebastiaan , Sleegers Kristel , Frisoni Giovanni B. , Wallin Anders , Lléo Alberto , Popp Julius , Martinez-Lage Pablo , Legido-Quigley Cristina , Barkhof Frederik , Zetterberg Henrik , Visser Pieter Jelle , Bertram Lars , Lovestone Simon , Nevado-Holgado Alejo J. 
Published: July 2021

Background and Objective: Plasma biomarkers for the diagnosis and stratification of Alzheimer’s disease (AD) are intensively sought. However, no plasma markers are well established so far for AD diagnosis. Our group has identified and validated various blood-based proteomic biomarkers relating to AD pathology in multiple cohorts. The study aims to conduct a meta-analysis based on our own studies to systematically assess the diagnostic performance of our previously identified blood biomarkers.Methods: To do this, we included seven studies that our group has conducted during the last decade. These studies used either Luminex xMAP or ELISA to measure proteomic biomarkers. As proteins measured in these studies differed, we selected protein based on the criteria that it must be measured in at least four studies. We then examined biomarker performance using random-effect meta-analyses based on the mean difference between biomarker concentrations in AD and controls (CTL), AD and mild cognitive impairment (MCI), MCI, and CTL as well as MCI converted to dementia (MCIc) and non-converted (MCInc) individuals.Results: An overall of 2,879 subjects were retrieved for meta-analysis including 1,053 CTL, 895 MCI, 882 AD, and 49 frontotemporal dementia (FTD) patients. Six proteins were measured in at least four studies and were chosen for meta-analyses for AD diagnosis. Of them, three proteins had significant difference between AD and controls, among which alpha-2-macroglobulin (A2M) and ficolin-2 (FCN2) increased in AD while fibrinogen gamma chain (FGG) decreased in AD compared to CTL. Furthermore, FGG significantly increased in FTD compared to AD. None of the proteins passed the significance between AD and MCI, or MCI and CTL, or MCIc and MCInc, although complement component 4 (CC4) tended to increase in MCIc individuals compared to MCInc.Conclusions: The results suggest that A2M, FCN2, and FGG are promising biomarkers to discriminate AD patients from controls, which are worthy of further validation.
Cerebrovascular Risk Factors in Possible or Probable Cerebral Amyloid Angiopathy, Modifier or Bystander?
Authors: Wagner Andrea , Maderer Jonas , Wilfling Sibylle , Kaiser Johanna , Kilic Mustafa , Linker Ralf A. , Schebesch Karl-Michael , Schlachetzki Felix 
Published: July 2021

Goal: Cerebral amyloid angiopathy (CAA) is a frequent cause of atypical intracerebral hemorrhage (ICH) in the elderly. Stroke risk factors such as arterial hypertension (AHT), atrial fibrillation (AFib), diabetes mellitus (DM), and renal dysfunction (RD) are increasingly apparent in these patients. In this retrospective study, we analyzed the presence of these stroke risk factors in different initial CAA presentations comprising cerebral microbleeds (CMB), acute ischemic stroke (AIS), cortical superficial hemosiderosis (cSS), or lobar ICH (LICH) and evaluated their influence on the initial clinical presentation of patients with CAA.Material and Methods: We identified patients with at least possible CAA defined by the modified Boston criteria admitted to the Department of Neurology or Neurosurgery from 2002 to 2018.Findings: In the overall cohort of 209 patients, we analyzed the correlation between the number of stroke risk factors and the initial clinical presentation of patients with CAA and could show the high multimorbidity of the collective. There are large differences between the subgroups with different initial clinical presentations, e.g., patients with CMB as initial CAA presentation have the highest number of cerebrovascular risk factors and recurrent AIS, whereas AFib is more frequent in the Neurosurgery Department.Conclusion: There is a distinct overlap between the subgroups of CAA manifestations and stroke risk factors that need to be verified in larger patient collectives. Since these comorbidities are likely to influence the clinical course of CAA, they represent possible targets for secondary prevention until specific treatment for CAA becomes available.
RBM10 Deficiency Is Associated With Increased Immune Activity in Lung Adenocarcinoma
Authors: Liu Bing , Wang Yaqi , Wang Han , Li Zhongwu , Yang Lujing , Yan Shi , Yang Xin , Ma Yuanyuan , Gao Xuan , Guan Yanfang , Yi Xin , Xia Xuefeng , Li Jingjing , Wu Nan 
Published: July 2021

IntroductionRBM10 is one of the frequently mutated genes in lung adenocarcinoma (LUAD). Previous studies have confirmed that RBM10 could suppress the disease progression and cell proliferation in LUAD, but its loss-of-function mutations are more frequent in early-stage disease and decrease with the advancement of the clinical stage. This is contradictory to its role of tumor suppressor. Here, we conducted a systematic analysis to elucidate whether there was other potential biological significance of RBM10 deficiency during the progression of LUAD.Materials and MethodsThe whole exome sequencing data of 39 tumor samples from early-stage LUADs (GGN cohort) and genomic and transcriptome data of the Cancer Genome Atlas (TCGA) LUAD cohort (TCGA_LUAD cohort) and a Chinese LUAD cohort (CHOICE_ADC cohort) were first obtained. Systematic bioinformatic analyses were then conducted to determine gene expression signature, immune infiltration levels and predicted immunotherapy response. Immunohistochemistry (IHC) was also conducted to validate the result of immune infiltration.ResultsThe mutation rate of RBM10 was significantly higher in the GGN cohort than that in the TCGA_LUAD and CHOICE_ADC cohorts. In both TCGA_LUAD and CHOICE_ADC cohorts, multiple immune related pathways were markedly enriched in RBM10 deficient group. Further analyses showed that tumors with RBM10 mutations displayed higher TMB, and LUADs with RBM10 deficiency also showed higher HLA expression levels, including many HLA class I and II molecules. Additionally, many immune cells, including myeloid dendritic cells, macrophages, neutrophils and CD8+T cells, showed higher infiltration levels in LUADs with RBM10 deficiency. Finally, some immune checkpoint molecules, such as PD-L1 and TIM-3, were highly expressed in RBM10 deficient population and the predicted immunotherapy response was calculated through TIDE algorithm, showing that IFNG expression, MSI score and CD8 expression were higher in RBM10 deficient group, while MDSC and M2 macrophage were lower in RBM10 deficient group.ConclusionOur study demonstrates that RBM10 deficient LUADs show higher HLA expression and immune cell infiltration, and some immune checkpoint molecules are also highly expressed. In brief, RBM10 deficiency could enhance anti-tumor immunity in LUAD.
Defining Unmet Need Following Lenalidomide Refractoriness: Real-World Evidence of Outcomes in Patients With Multiple Myeloma
Authors: Lecat Catherine S. Y. , Taube Jessica B. , Wilson William , Carmichael Jonathan , Parrish Christopher , Wallis Gabriel , Kyriakou Charalampia , Lee Lydia , Mahmood Shameem , Papanikolaou Xenofon , Rabin Neil K. , Sive Jonathan , Wechalekar Ashutosh D. , Yong Kwee , Cook Gordon , Popat Rakesh 
Published: July 2021

BackgroundThe treatment paradigm for multiple myeloma (MM) continues to evolve with the development of novel therapies and the earlier adoption of continuous treatments into the treatment pathway. Lenalidomide-refractory patients now represent a challenge with inferior progression free survival (PFS) reported to subsequent treatments. We therefore sought to describe the natural history of MM patients following lenalidomide in the real world.MethodsThis was a retrospective cohort review of patients with relapsed MM who received lenalidomide-based treatments in the U.K. Data were collected for demographics, subsequent therapies, treatment responses, survival outcomes and clinical trial enrollment.Results198 patients received lenalidomide-based treatments at a median of 2 prior lines of therapy at a median of 41 months (range 0.5-210) from diagnosis. 114 patients (72% of 158 evaluable) became refractory to lenalidomide. The overall survival (OS) after lenalidomide failure was 14.7 months having received between 0-6 subsequent lines of therapy. Few deep responses were observed with subsequent treatments and the PFS to each further line was &lt; 7 months. There was a steep reduction in numbers of patients able to receive further treatment, with an associated increase in number of deaths. The OS of patients progressing on lenalidomide who did not enter a clinical trial incorporating novel agents was very poor (8.8 months versus 30 months, p 0.0002), although the trials group were a biologically fitter group.ConclusionThese data demonstrate the poor outcomes of patients failing lenalidomide-based treatments in the real world, the highlight need for more effective treatments.
The Occurrence of Sexual Risk Behaviors and Its Association With Psychological Well-Being Among Kenyan Adolescents
Authors: Ssewanyana Derrick , Abubakar Amina , Mabrouk Adam , Kagonya Vincent A. , Nasambu Carophine , Dzombo Judith Tumaini , Angwenyi Vibian , Kabue Margaret , Scerif Gaia , Newton Charles R. 
Published: July 2021

Objective: Sexual risk behavior during adolescence is an important public health problem. Self-esteem and hopefulness are potentially important psychological factors that may play a role in the behavioral regulation mechanisms of adolescents. These factors are inadequately explored in sub-Saharan Africa. This study aimed at exploring patterns and associated factors for sexual risk behavior (SRB), self-esteem, and hopefulness among adolescents from a resource-poor setting in Kenya.Method: A cross-sectional study conducted in 2019 among 296 adolescents (12–17 years old) from rural Kilifi (n = 133) and urban informal settings of Nairobi (n = 163) in Kenya. Participants completed the Kilifi Health Risk Behavior Questionnaire, Rosenberg self-esteem questionnaire, and Hope scale administered via computerized tablets. A binary outcome variable based on the experience of adolescents of at least one of the five forms of SRB: transactional sex, sexual violence, intergenerational sex, early sexual debut, and condom non-use was generated. Bi-variate analysis was conducted to summarize various social-demographic and psychosocial factors. A multivariable logistic regression model was fitted to investigate factors associated with the occurrence of SRB, self-esteem, and hopefulness among adolescents.Results: About 13% of the participants had experienced a form of SRB, and among these, 36% reported co-occurrence of multiple forms of SRB. Adolescent SRB was largely characterized by having experienced sexual violence, as well as intergenerational and transactional sex. Higher scores of hopefulness were reported among adolescents who never experienced SRB (P = 0.03) at bivariate analysis level. However, both self-esteem and hopefulness were not significantly associated with the occurrence of SRB in the adjusted logistic regression analysis. Having depressive symptoms (Adj. OR = 3.8, 95% CI: 1.39–10.4), feeling unsafe in the neighborhood (Adj. OR = 3.4, 95% CI: 1.6–7.2), and being in higher compared with lower primary education level (Adj. OR = 0.3, 95% CI: 0.1–0.8) were statistically significantly associated with the occurrence of SRB.Conclusion: Targeted reproductive health interventions, designed with the cognizance of structural and social drivers of adolescent SRB, are needed to concurrently tackle multiple forms of SRB. It is important to integrate mental health promotion within these interventions. More research is needed to understand the mechanisms and implications of self-esteem and hopefulness for adolescent sexual and reproductive health.
Estimating Short- and Long-Term Associations Between Air Quality Index and COVID-19 Transmission: Evidence From 257 Chinese Cities
Authors: Cao Ru , Wang Yuxin , Pan Xiaochuan , Jin Xiaobin , Huang Jing , Li Guoxing 
Published: July 2021

Objectives: To evaluate the long- and short-term effects of air pollution on COVID-19 transmission simultaneously, especially in high air pollution level countries.Methods: Quasi-Poisson regression was applied to estimate the association between exposure to air pollution and daily new confirmed cases of COVID-19, with mutual adjustment for long- and short-term air quality index (AQI). The independent effects were also estimated and compared. We further assessed the modification effect of within-city migration (WM) index to the associations.Results: We found a significant 1.61% (95%CI: 0.51%, 2.72%) and 0.35% (95%CI: 0.24%, 0.46%) increase in daily confirmed cases per 1 unit increase in long- and short-term AQI. Higher estimates were observed for long-term impact. The stratifying result showed that the association was significant when the within-city migration index was low. A 1.25% (95%CI: 0.0.04%, 2.47%) and 0.41% (95%CI: 0.30%, 0.52%) increase for long- and short-term effect respectively in low within-city migration index was observed.Conclusions: There existed positive associations between long- and short-term AQI and COVID-19 transmission, and within-city migration index modified the association. Our findings will be of strategic significance for long-run COVID-19 control.