bluuebunny/crossref_metadata_2025_split huggingface dataset:
Future temperature and salinity in Puget Sound, Washington State, under CMIP6 climate change scenarios
Authors: Walker Stevie , Morzaria-Luna Hem Nalini , Kaplan Isaac , Petatán-Ramírez David 
Published: September 2021

Abstract
In Washington State, climate change will reshape the Puget Sound marine ecosystem through bottom-up and top-down processes, directly affecting species at all trophic levels. To better understand future climate change effects on sea surface temperature and salinity in Puget Sound, we used empirical downscaling to derive high-resolution time series of future sea surface temperature and salinity. Downscaling was based on scenario outputs of two coarse-resolution General Circulation Models, GFDL-CM4 and CNRM-CM6-1-HR, developed as part of the Coupled Model Intercomparison Project Phase 6 (CMIP6). We calculated 30-year climatologies for historical and future simulations, calculated the anomalies between historical and future projections, interpolated to a high resolution, and applied the resulting downscaled anomalies to a Regional Ocean Modeling System (ROMS) time series, yielding short-term (2020–2050) and long-term (2070–2100) delta-downscaled forecasts. Downscaled output for Puget Sound showed temperature and salinity variability between scenarios and models, but overall, there was strong model agreement. Model variability and uncertainty was higher for long-term projections. Spatially, we found regional differences for both temperature and salinity, including higher temperatures in the South Basin of Puget Sound and higher salinity in the North Basin. This study is a first step to translating CMIP6 outputs to higher resolution predictions of future conditions in Puget Sound. Interpreting downscaled projections of temperature and salinity in Puget Sound will help inform future ecosystem-based management decisions, such as supporting end-to-end ecosystem modeling simulations and assessing local-scale exposure risk to climate change.
Research on A Novel MPP Tracking Strategy Reaction to Rapid Change of Light Intensity
Authors: Sun Chunhu , Ling Jing , Liu Tingting 
Published: September 2021

Abstract
For the misjudgment and oscillation in the traditional MPP tracking process when the light intensity changes rapidly, a novel improved optimal gradient method for power prediction is proposed, based on the research of the traditional power prediction method and the optimal gradient method.In this article, the working principle, advantages and disadvantages of the traditional power prediction method and optimal gradient method are firstly analyzed; then a novel power prediction method with high-precision power calculation, as we as an improved optimal gradient method are proposed. Finally, Simulink simulation analysis is carried out on the proposed MPP tracking strategy when the light intensity changes rapidly. The simulation results show that when the light intensity changes rapidly, the novel power prediction improvement is adopted. The optimal gradient method can not only eliminate the misjudgment phenomenon and oscillation phenomenon, but also the power calculation is more accurate. The introduction of the variable step factor can make the system output more power, which can well realize the fast tracking of MPP.
Sarcocystis Attenuati N. Sp. (Apicomplexa: Sarcocystidae) Infecting the Asian Gray Shrew, Crocidura Attenuata (Insectivora: Soricidae), in China
Authors: hu junjie , Sun Jun , Guo Yanmei , Zeng Hongxia , Zhang Yunzhi , Tao Jianping 
Published: September 2021

Abstract
Background: There are limited data on Sarcocystis in insectivores. The Asian gray shrew, Crocidura attenuata, is one of the most common species of insectivores in the family Soricidae distributed in South Asia and Southeast Asia. To date, Sarcocystis has never been recorded in this host.Methods: Tissues from 42 Asian gray shrews were collected in China in 2017 and 2018. Sarcocysts were observed using light (LM) and transmission electron microscopy (TEM). To complete the parasite life cycle, muscle tissues of the host infected with sarcocysts were force-fed to two beauty rat snakes, Elaphe taeniura. Individual sarcocysts from different Asian gray shrews and oocysts/sporocysts isolated from the small intestines and feces of the experimental snakes were selected for DNA extraction, and seven genetic markers, including two nuclear loci (18S rDNA and ITS1), three mitochondrial genes (cox1, cox3 and cytb), and two apicoplastic genes (rpoB and clpC), were amplified, sequenced and analyzed.Results: Sarcocysts were found in 17 of 42 (40.5%) Asian gray shrews. Under LM, the microscopic sarcocysts were exhibited saw-tooth-like protrusions measuring 3.3–4.5 μm. Ultrastructurally, the sarcocyst wall contained numerous lancet- or leaf-like villous protrusions, similar to type 9h. The experimental beauty rat snakes shed oocysts/sporcysts measuring 11.9–16.7 × 9.2–10.6 μm with a prepatent period of 10 to 11 days. Comparing these sequences with those previously deposited in GenBank revealed that the 18S rDNA sequences and cox1 sequences shared the highest similarity with those of S. scandentiborneensis recorded in tree shrews, Tuaia minor and T. tana (i.e., 97.6–98.3% and 100% identity, respectively). Phylogenetic analysis based on 18S rDNA, ITS1 or cox1 sequences revealed that this parasite formed an independent clade with Sarcocystis spp. that utilize small animals as intermediate hosts and snakes as the known or presumed definitive host. On the basis of morphological and molecular characteristics and host specificity, the parasite was proposed as a new species, named S. attenuati.Conclusions: Sarcocysts were recorded in Asian gray shrews for the first time. The sarcocysts were characterized morphologically and molecularly. The 18S rDNA and cox1 sequences of S. attenuati, named in the present study, shared the highest identities with those of S. scandentiborneensis. However, the sarcocysts of the two species of Sarcocystis were quite different under LM and TEM. Based on experimental infection, beauty rat snakes have been proven to be a definitive host of S. attenuati. As more species of Sarcocystis from insectivores and other small mammals are properly morphologically and molecularly characterized, we may gain a better understanding of the biodiversity, host specificity and evolution of Sarcocystis in the future.
Tumor Cell-Derived Exosomal hsa_circ_0017252 Suppresses the Tumorigenesis of Gastric Cancer Through Suppressing M2 Polarization of Macrophage
Authors: Song Jin , Xu Xiaolong , He Shasha , Wang Ning , Bai Yunjing , Li Bo , Zhang Shengsheng 
Published: September 2021

Abstract
Background: Gastric cancer (GC) is a source of global cancer death. MiR-17-5p is reported to regulate the tumorigenesis of GC. Meanwhile, hsa_circ_0017252 is known to be upregulated in GC. However, the relation between hsa_circ_0017252 and miR-17-5p in GC remains unclear. Methods: Cell viability, migration and invasion were tested by CCK-8 and transwell assay, respectively. Gene expressions were detected by RT-qPCR, and the protein levels in cells or exosomes were tested by western blot. The efficiency of exosomes isolation was investigated by transmission electron microscope (TEM) and nanoparticle tracking analysis (NTA). Meanwhile, cell apoptosis was tested by flow cytometry. In vivo model was constructed to assess the function of MKN45 cells-derived exosomal hsa_circ_0017252 in GC. Results: Hsa_circ_0017252 was verified to be significantly downregulated in GC tissues. Hsa_circ_0017252 upregulation significantly decreased the viability and migration of GC cells, and hsa_circ_0017252 could bind with miR-17-5p. Additionally, exosomal hsa_circ_0017252 reversed the polarization of M2 macrophages, and the polarized macrophages decreased the GC cell invasion. Furthermore, exosomes with upregulated hsa_circ_0017252 suppressed the tumor growth of GC. Conclusion: Delivery of hsa_circ_0017252 by GC cells-derived exosomes inhibits the tumorigenesis of GC through reversing M2 polarization of macrophages. Thus, our finding might provide a new method for GC treatment.
Factors Related to Serum Lipids Using Multilevel Quantile Mixed Model: Analysis of Nationwide STEPs Survey 2016
Authors: Mohseni Parisa , Khalili Davood , Djalalinia Shirin , Farzadfar Farshad , Mehrabi Yadollah 
Published: September 2021

Abstract
Backgroundlipid disorder is a modifiable risk factor for diseases related to plaque formation in arteries such as heart attack, stroke and peripheral vascular diseases. Identifying related factors and diagnosis and treatment in time reduces the incidence of non - communicable diseases. The aim of this study was to determine factors associated with lipids based on a national survey data.MethodsData of 16757 individuals aged 25–64 years obtained from the Iranian national cross-sectional study of the risk factors of non-Communicable disease (STEPs) performed in 2016, through multi-stage random sampling, were analyzed. Because of clustered, hierarchical and skewed form of the data, factors related to total Cholesterol (TC), Triglycerides (TG), LDL-C, HDL-C, TG/HDL-C, TC/HDL-C, LDL-C/HDL-C were determined applying multilevel quantile mixed model. Parameters of the model were estimated on the basis of random effect of the province as well as urban or rural area for 10th, 25th, 50th, 75th and 90th quantiles. Statistical analyses were performed by R software version 4.0.2.ResultsSignificant relationship was found between age, BMI, waist circumference, diabetes, hypertension, smoking, physical activity, education level, and marital status with TC, LDL-C, HDL-C, LDL-C and LDL-C/HDL-C. With increasing BMI and WC, subjects had higher levels of serum lipids, especially in higher quantiles of lipid levels. Lipid levels were significantly increased among smokers and those with diabetes or hypertension. The random effects were also significant showing that there is a correlation between the level of lipids in provincial habitants as well as urban and rural areas.ConclusionThis study showed that the effect of each factor varies depending on the centiles of the lipids. Significant relationship was found between sociodemographic, Behaviors and anthropometric indices with lipid parameters.
Clinicopathological and Genetic Analyses of Small Cell Neuroendocrine Carcinoma of the Prostate: Histological Features for Accurate Diagnosis and Toward Future Novel Therapies Short Running Title: Characteristics of Prostatic Small Cell Neuroendocrine Carcinoma
Authors: Ida Arika , Okubo Yoichiro , Kasajima Rika , Washimi Kota , Sato Shinya , Yoshioka Emi , Osaka Kimito , Suzuki Takahisa , Yamamoto Yayoi , Yokose Tomoyuki , Kishida Takeshi , Miyagi Yohei 
Published: September 2021

Abstract
Differentiating small cell neuroendocrine carcinoma (SCNC) of the prostate from prostatic cancer with diffuse neuroendocrine (NE) differentiation based on morphological features alone can be challenging. Given that treatment strategies vary depending on histological type, accurate diagnosis is critical. This study firstly aimed to identify the accurate diagnostic factors for primary prostate NE tumors. Furthermore, the possibility of novel treatment strategies through genetic analysis is also an aim. Specifically, prostate biopsies conducted in our hospital between January 2017 and May 2020 were included. As a result seven cases of SCNC and four cases of prostatic cancer with diffuse NE differentiation were identified. No significant differences in serum neuron-specific enolase (NSE), pro-gastrin-releasing peptide (ProGRP), and prostate-specific antigen (PSA) levels were observed between SCNC and adenocarcinoma with diffuse NE differentiation. The Ki-67 labeling index was significantly higher and PSA immunoreactivity tended to be lower in SCNC. Genetic analysis did not detect any ALK mutations but confirmed several other mutations, including those of PIK3CA and TP53. In conclusion, given the heterogeneity in serum NE markers in SCNC, diagnosis based on these markers alone is difficult. A high Ki-67 labeling index and low PSA immunoreactivity may be useful for diagnosing, but p53 immunoreactivity is insufficient to distinguish these tumors. Although further studies are required to interpret the results of the genetic analysis involving ALK, PIK3CA, and TP53 mutations, the results of our genetic analysis suggest that PIK3CA mutations in SCNC of the prostate may provide a novel therapeutic strategy.
Seismic activity in the focus of the Uglegorsk earthquakes, Sakhalin Island in relevance to intensive development of coal deposits
Authors: Kostylev Dmitry , Boginskaya Natalya , Zakupin Alexander 
Published: September 2021

Abstract
Induced seismicity is an increase in seismic activity caused by the human engineering. An example of such activity is the mineral exploration, large water reservoirs construction, exploitation of underground oil and gas storages, etc. The authors studied the seismicity in the Uglegorsky district of Sakhalin region, where the Solntsevskoye brown coal field is located, which is the most promising in the island. Its area is over 100 sq. km, and productive strata of the Verkhneduiskaya formation with a thickness of up to 600 m contains 12 coal seams, 8 of which are working. Active mining of brown coal is carried out at the Solntsevsky coal mine, and blasting operations are performed on a large scale, that, as a result, does not exclude the relation of the seismic process to technogenic seismicity. The earthquake recurrence curves for two decades beginning from 2000 to the present were constructed in the work to compare the characteristics of the seismic regime in the studied area. The difference in the slope angle of recurrence graph during the period of 2011-2020 (the period of the most active development of the Solntsevsky coal mine) from the previous decade is quite significant. The maps of spatiotemporal distribution of seismic events epicenters in the vicinity of Solntsevsky coal mine are constructed. The contraction of zones of seismic events concentration to the mining areas, first of all to the Solntsevsky coal mine, have been found. Such a combination allows us to talk about an increase in seismicity of the region during the last years and change in its character from the natural to a mixed natural and technogenic. The focal mechanisms of the largest earthquakes occurred in the Uglegorsky district have been constructed in order to prove the change in seismicity character and reasons for the earthquake occurrence in the studied area. The mechanisms of seismic events of 2020 are classified as strike-slip faults, that is not character for the most earthquakes on the territory of Sakhalin Island. The authors made an attempt to determine the regularities of the parameters of the produced blasts and earthquakes through dynamic parameters of the seismic events foci by means of studying the frequency content of earthquakes and blasts in order to determine a corner frequency from the focal velocity spectrum.
WITHDRAWN: Plasma-based Endogenous Metabolomics Profiling of High-Risk Human Papillomavirus and Their Emerging Roles in the Progression of Cervical Cancer
Authors: Wang Qin , Xu Min , Chen Tingting , Chen Jing , Zhang Runjie , Qiu Jin 
Published: September 2021

Abstract
Objective: High-risk human papillomavirus (HR-HPV) is the main etiological factor for cervical cancer. Accumulating evidence has suggested that the active role of metabolites in the initiation and progression of cancers. This study was to explore the metabolic profiles of HR-HPV infection and their potential functions in cervical cancer.Methods: Non-targeted metabolomics approach was used to detect metabolic alterations in the plasma obtained from HPV-16 positive (HPV16 (+)), HPV-18 positive (HPV18 (+)) and HPV negative (CTL) individuals, followed by CCK8 experiment to detect the effect of different metabolites on the proliferation of Hela and GH354. A cell migration test then verified significant metabolites on the migration of Hela and GH354. Q RT-qPCR and western blot were used to detect malignant progression related mRNA and protein expression levels of cervical cancer.Results: HR-HPV groups shared 24 dysregulated metabolites (such as amino acids, ceramides, glycerophosphocholines). Further experiments showed ceramide species, including C8 inhibits cervical cancer cells proliferation and migration in vitro. In contrast, C12 significantly enhanced cervical cancer cells proliferation and migration in vitro. Protein and mRNA expressions indicated C8 and C12 were related to the malignant behavior of cervical cancer in vitro. The underlying mechanism demonstrated that C8 intervention inhibited proliferation and migration in cervical cancer cells via the MAPK/JNK signaling pathway, while C12 intervention promoted proliferation and migration in cervical cancer cells via the MAPK/ERK signaling pathway. These findings may contribute to the treatment of HR-HPV-induced cervical cancer by intervening in its initiation and progression.Conclusion: Our study shed some light on how metabolites influenced the relationship between HR-HPV oncogenic capability and metabolic phenotype change and identify species C8 and C12 as critical lipid metabolites that modulate cervical cancer cell’s function.
Mental Health Experiences With COVID-19 Public Health Measures in an Alberta First Nations Community
Authors: Lee Cerina , Wozniak Lisa A , Soprovich Allison L , Sharma Vishal , Healy Bonnie , Samanani Salim , Eurich Dean T 
Published: September 2021

Abstract
Background: The First Nations (FN) peoples of Canada have experienced health inequalities intergenerationally and life course due to colonization, significant social, physical and economic challenges. Consequently, the COVID-19 pandemic has resulted in an exacerbation of health inequities and mental health issues for FN communities around the world. This study aims to understand their experiences related to mental health in an Alberta FN community during the early stages of the pandemic. Methods: In partnership with FN leadership, the study implemented an online cross-sectional survey. Adults from a large FN community in Alberta, Canada, were asked to complete a survey, including two mental health-related screening questionnaires: 1) Generalized Anxiety Disorder-2 item; and 2) Patient Health Questionnaire-2 item. In addition, respondents could provide responses to open-ended questions about their experiences. Results: Among 106 respondents, 95 (89.6%) finished the survey; 18% of adults screened positive for depressive symptoms (score of 3 or greater) and reported difficulty following public health advice for using hand sanitizer, maintaining social distancing, or self-isolating. 21% of adults screened positive for symptoms of anxiety (score of 3 or greater) and reported difficulty maintaining social distance, self-isolating, obtaining food and clothing, or meeting other basic living requirements. Conclusions: FN communities may be disproportionately affected by COVID-19, and may experience exacerbated symptoms of anxiety, depression and overall poor mental health and well-being in response to public health advice. Additional supports and services, including for mental health, should be considered for FN in the context of COVID-19 public health measures.
Based on network pharmacology, using methods such as molecular docking and gene difference analysis, small molecule compounds for the treatment of lung adenocarcinoma and lung squamous cell carcinoma were screened in saussurea involucrata, and new therapeutic targets were discovered
Authors: Zhang Dongdong , Zhang Tieying , Zhang Yao , Li Zhongqing , Li He , Zhang Yueyang , Liu Chenggong , Han Zichao , Li Jin , Zhu Jianbo 
Published: September 2021

Abstract
Purpose: Based on systemic pharmacology and network pharmacology, to evaluate the therapeutic effect of saussurea involucrata (SAIN) on lung adenocarcinoma and lung squamous cell carcinoma (LUAD&LUSC) through clinical sample genetic difference analysis and compound-target molecular docking, and discover new The target of prevention or treatment of LUAD&LUSC. Materials and methods: Using the TCM System Pharmacology Database (TCMSP) as the starting point for preliminary selection of ingredients and targets (OB≥30%, DL≥0.18, n=9), with the GDC database as the end point, using Cytoscape 3.8, TBtools 1.082, AutoDock 4.2.6, R 4.0.4, PyMol and other tools have conducted a preliminary screening of the ingredients and targets of SAIN. In order to further screen the effective ingredients and targets, we used clinical samples from LUAD and LUSC from TCGA and GEPIA to perform genetic difference analysis (n=6), and perform biological process (BP) analysis (FDR) on these targets. ≤0.05, n=6), KEGG pathway analysis (FDR≤0.05, n=6), protein interaction network (PPI) analysis (n=6) and compounds-targets-pathways network analysis (n=6), obtain biological processes, disease pathways and various compounds regulated by targets-the relationship between targets and pathways. Through the precise molecular docking of ingredients and targets, we further screened the targets of the effective ingredients of SAIN (affinity≤-7.0 kcal/mol, H-Bond dist≤3.0, n=6) and visualized the data, Then these targets were verified by using PSORTⅡ, CELLO and BUSCA databases for subcellular localization prediction (n=6). Finally, use the large amount of TCGA clinical data provided by Cbiportal for the prognostic survival analysis of LUAD and LUSC for the genes obtained through the screening. , And consult a large number of documents to verify the results.Results: After screening, comparing, analyzing and verifying a series of data, it is finally confirmed that there are three main active ingredients in SAIN. They are Quercetin, Luteolin and Kaempferol, which mainly act on 6 protein targets. The target mainly regulates 20 signal pathways including Pathways in cancer, Transcriptional misregulation in cancer, EGFR tyrosine kinase inhibitor resistance, Adherens junction, IL-17 signaling pathway, Melanoma, Non-small cell lung cancer and MicroRNAs in cancer. The preventive or therapeutic effects of LUSC. Conclusion: There are three active compounds of Q, L and K in SAIN, which play a role in the treatment and prevention of NSCLC by directly or indirectly regulating the expression of genes such as MMP1, MMP3 and EGFR.
Environmental quality standards for diclofenac derived under the european water framework directive: 1. Aquatic organisms
Authors: Leverett Dean , Merrington Graham , Crane Mark , Ryan Jim , Wilson Iain 
Published: September 2021

Abstract
Diclofenac is a nonsteroidal anti-inflammatory human and veterinary medicine widely detected in European surface waters, especially downstream from Wastewater Treatment Plants. Veterinary uses of diclofenac in Europe are greatly restricted, so wastewater is the key exposure route for wildlife. Proposed Environmental Quality Standards (EQS) which include an assessment of toxicity to aquatic organisms are under consideration by the European Commission (EC) to support the aims of the Water Framework Directive (WFD). The EC approach favours the use of a deterministic (single test value and an assessment factor) approach to the derivation of a direct toxicity EQS for diclofenac, resulting in an EQS of 0.040 µg L− 1 based on a single mesocosm study. In this paper, we discuss potential issues with this approach with respect to the EC’s own guidance on EQS derivation and derive an evidence-driven alternative EQS of 0.126 µg L− 1 using a probabilistic (Species Sensitivity Distribution) approach that accounts for all of the reliable and relevant data and is in accordance with the guidance. Europe-wide freshwater monitoring data for diclofenac are used in an indicative compliance assessment using the EC and the alternative evidence-driven EQS. The implications of using only some data to derive an EQS that does not adhere to the guidance, compared to a guidance compliant approach that uses all the data available is also discussed.