bluuebunny/crossref_metadata_2025_split huggingface dataset:
INFERR-Iron infusion in haemodialysis study: INtravenous iron polymaltose for First Nations Australian patients with high FERRitin levels on haemodialysis—a protocol for a prospective open-label blinded endpoint randomised controlled trial
Authors: Majoni Sandawana William , Nelson Jane , Germaine Darren , Hoppo Libby , Long Stephanie , Divakaran Shilpa , Turner Brandon , Graham Jessica , Cherian Sajiv , Pawar Basant , Rathnayake Geetha , Heron Bianca , Maple-Brown Louise , Batey Robert , Morris Peter , Davies Jane , Fernandes David , Sundaram Madhivanan , Abeyaratne Asanga , Wong Yun Hui Sheryl , Lawton Paul D. , Taylor Sean , Barzi Federica , Cass Alan ,   for the INFERR study Group
Published: December 2021

Abstract
Background
The effectiveness of erythropoiesis-stimulating agents, which are the main stay of managing anaemia of chronic kidney disease (CKD), is largely dependent on adequate body iron stores. The iron stores are determined by the levels of serum ferritin concentration and transferrin saturation. These two surrogate markers of iron stores are used to guide iron replacement therapy. Most Aboriginal and/or Torres Islander Australians of the Northern Territory (herein respectfully referred to as First Nations Australians) with end-stage kidney disease have ferritin levels higher than current guideline recommendations for iron therapy. There is no clear evidence to guide safe and effective treatment with iron in these patients. We aim to assess the impact of intravenous iron treatment on all-cause death and hospitalisation with a principal diagnosis of all-cause infection in First Nations patients on haemodialysis with anaemia, high ferritin levels and low transferrin saturation

Methods
In a prospective open-label blinded endpoint randomised controlled trial, a total of 576 participants on maintenance haemodialysis with high ferritin (> 700 μg/L and ≤ 2000 μg/L) and low transferrin saturation (< 40%) from all the 7 renal units across the Northern Territory of Australia will be randomised 1:1 to receive intravenous iron polymaltose 400 mg once monthly (200 mg during 2 consecutive haemodialysis sessions) (Arm A) or no IV iron treatment (standard treatment) (Arm B). Rescue therapy will be administered when the ferritin levels fall below 700 μg/L or when clinically indicated. The primary outcome will be the differences between the two study arms in the risk of hospitalisation with all-cause infection or death. An economic analysis and several secondary and tertiary outcomes analyses will also be performed.

Discussion
The INFERR clinical trial will address significant uncertainty on the safety and efficacy of iron therapy in First Nations Australians with CKD with hyperferritinaemia and evidence of iron deficiency. This will hopefully lead to the development of evidence-based guidelines. It will also provide the opportunity to explore the causes of hyperferritinaemia in First Nations Australians from the Northern Territory.

Trial registration
This trial is registered with The Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN12620000705987. Registered 29 June 2020.
Interrelationship between secondary metabolites and antioxidant capacities of Centella asiatica using bivariate and multivariate correlation analyses
Authors: Shin Hyun Young , Kim Hoon , Jung Soontag , Jeong Eun-Jin , Lee Kyung-Haeng , Bae Yun-Jung , Suh Hyung Joo , Jang Keum-Il , Yu Kwang-Won 
Published: December 2021

AbstractCentella asiatica L. Urban (CA) is a valuable medicinal herb that contains various bioactive secondary metabolites. In the present study, the harvested CA was divided into whole plant and leaf parts, and were heated-air-dried or freeze-dried. The dried CA was extracted under various extraction conditions to obtain 16 extracts, and their antioxidant activities were examined. Twelve types of secondary metabolites (five polyphenolic acids, four triterpenoids, and three flavonoids) were quantified in each extract. Finally, the intercorrelations between secondary metabolites and antioxidant activities were analyzed through statistical analyses, such as Pearson correlation coefficient, scatter plotting, and principal component analysis. The antioxidant capacities of CA might be primarily influenced by major triterpenoids such as madecassoside and asiaticoside, which showed high content in the ethanol extracts of freeze-dried leaf parts. The present study provides a valuable groundwork for the development of optimal extraction processes for C. asiatica L. as an antioxidant material.
The rise of contract cheating during the COVID-19 pandemic: a qualitative study through the eyes of academics in Kuwait
Authors: Erguvan Inan Deniz 
Published: December 2021

AbstractContract cheating has gone rampant in higher education recently. When institutions switched to online learning during the COVID-19 pandemic, the percentage of contract cheating students climbed to unprecedented levels. Essay mills saw the lack of face-to-face interaction and proctoring on campus as an opportunity and used aggressive marketing methods to attract students. This study asked the opinions of 20 faculty members working in the English departments of private higher education institutions in Kuwait regarding contract cheating through interviews. The data was analyzed with MAXQDA 2020. The findings show that all faculty members can recognize contract cheating easily. Most of them see contract cheating as a serious problem in the higher education system, a threat to the reliability of language assessment, triggered by laziness, the social pressure to graduate with a high GPA, and exacerbated by the cheating opportunities in online education. Academics have developed certain individual strategies in their courses to curb the number of contract cheating students; however, institutional measures differ, and in some, there are no measures or sanctions on contract cheating students.
Revitalizing the ethanologenic bacterium Zymomonas mobilis for sugar reduction in high-sugar-content fruits and commercial products
Authors: Hu Mimi , Chen Xiangyu , Huang Ju , Du Jun , Li Mian , Yang Shihui 
Published: December 2021

AbstractThe excessive consumption of sugars can cause health issues. Different strategies have been developed to reduce sugars in the diets. However, sugars in fruits and commercial products may be difficult to reduce, limiting their usage among certain populations of people. Zymomonas mobilis is a generally recognized as safe (GRAS) probiotic bacterium with the capability to produce levan-type prebiotics, and thrives in high-sugar environments with unique characteristics to be developed for lignocellulosic biofuel and biochemical production. In this study, the sugar reduction capabilities of Z. mobilis ZM4 were examined using two fruits of pear and persimmon and three high-sugar-content commercial products of two pear pastes (PPs) and one Chinese traditional wine (CTW). Our results demonstrated that Z. mobilis ZM4 can utilize sugars in fruits with about 20 g/L ethanol and less than 5 g/L sorbitol produced within 22 h using pears, and about 45 g/L ethanol and 30 g/L sorbitol produced within 34 h using persimmons. When PPs made from pears were used, Z. mobilis can utilize nearly all glucose (ca. 60 g/L) and most fructose (110 g/L) within 100 h with 40 ~ 60 g/L ethanol and more than 20 g/L sorbitol produced resulting in a final sorbitol concentration above 80 g/L. In the high-sugar-content alcoholic Chinese traditional wine, which contains mostly glucose and ethanol, Z. mobilis can reduce nearly all sugars with about 30 g/L ethanol produced, resulting in a final ethanol above 90 g/L. The ethanol yield and percentage yield of Z. mobilis in 50 ~ 60% CTW were 0.44 ~ 0.50 g/g and 86 ~ 97%, respectively, which are close to its theoretical yields—especially in 60% CTW. Although the ethanol yield and percentage yield in PPs were lower than those in CTW, they were similar to those in fruits of pears and persimmons with an ethanol yield around 0.30 ~ 0.37 g/g and ethanol percentage yield around 60 ~ 72%, which could be due to the formation of sorbitol and/or levan in the presence of both glucose and fructose. Our study also compared the fermentation performance of the classical ethanologenic yeast Saccharomyces cerevisiae BY4743 to Z. mobilis, with results suggesting that Z. mobilis ZM4 had better performance than that of yeast S. cerevisiae BY4743 given a higher sugar conversion rate and ethanol yield for sugar reduction. This work thus laid a foundation for utilizing the advantages of Z. mobilis in the food industry to reduce sugar concentrations or potentially produce alcoholic prebiotic beverages.
Graphical Abstract
Citrine Wagtail migration on the Indo-European flyway: a first geolocator track reveals alternative migration route and endurance flights to cross ecological barriers
Authors: Korniluk Michał , Białomyzy Paweł , Grygoruk Grzegorz , Tumiel Tomasz , Świętochowski Piotr , Wereszczuk Marcin 
Published: December 2021

AbstractMost long-distance migrating passerines that breed in Europe spend their winters in Africa, with only a few species migrating eastward to spend the non-breeding period in South Asia. The use of the Indo-European flyway is rare and has been poorly studied so far. However, it is extremely interesting as within that system we are currently witnessing a recent range expansion of European breeding long distance migrants and thus the lengthening of migration routes. It may therefore conceal a unique migratory strategies and behaviour that can help us to understand the underlying factors and mechanisms determining the evolution of migration routes, strategies and breeding range extinction. Based on light-level geolocator we reveal a first track of the Citrine Wagtail (Motacilla citreola) migration, providing insight into the migration pattern, timing and behaviour of the species that recently has extended its migration routes. Unexpectedly, the studied individual did not retrace a recent range expansion that runs north and east from the Caspian Sea but followed a migration route running south form the Caspian sea, suggesting possible presence of an alternative species range expansion. The overall migration distance between the breeding site in Poland and the non-breeding site in Pakistan was about 10,420 km and included two endurance movement phases (920 and 2240 km) covering 30% of the whole journey length, with an average movement speed of 574 km/day. We explain this migration behaviour as an adaptation for crossing the ecological barriers imposed by arid environments.
Choroidal thickness and choriocapillaris vascular density in myopic anisometropia
Authors: Liu Xinting , Lin Zhiyi , Wang Feifu , Peng Xiaoyi , He Wenwen , Chen Dan , Shen Meixiao , Lu Fan , Jiang Jun 
Published: December 2021

Abstract
Background
This study aims to examine interocular differences in the choroidal thickness and vascular density of the choriocapillaris in anisometropic myopes and to further explore the relationship between choroidal blood flow and myopia.

Methods
The sample comprised 44 participants with anisometropic myopia, aged 9 to 18 years, with normal best-corrected visual acuity. All participants underwent a series of examinations, including spherical equivalent refraction (SER) and axial length (AL), measured by a Lenstar optical biometer and optical coherence tomography angiography (OCTA) scanner. OCT measured the choroidal thickness, vascular density, and flow voids of the choriocapillaris, and a customized algorithm was implemented in MATLAB R2017a with the post-correction of AL. The choroidal thickness was measured at the fovea and 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mm nasally, temporally, inferiorly, and superiorly to the fovea. The vascular density and the flow voids of the choriocapillaris were measured at a 0.6-mm-diameter central circle, and the 0.6–2.5 mm diameter circle in the nasal, temporal, inferior, and superior regions. Repeated-measured ANOVAs were used to analyze the interocular differences. Partial correlations with the K value and age adjustments were used to study the relationships between the choroidal thickness, the choriocapillaris vascular density and flow voids, the SER and AL.

Results
The choroidal thickness of the more myopic eyes was significantly thinner than less myopic eyes (P ≤ 0.001), and the flow voids in the more myopic eyes were more than less myopic eyes (P = 0.002). There was no significant difference in the vascular density of the choriocapillaris between the more and less myopic eyes (P = 0.525). However, when anisometropia was more than 1.50 D, the vascular density of choriocapillaris in the more myopic eyes was significantly less than the less myopic eyes (P = 0.026). The interocular difference of the choroidal thickness was significantly correlated with the interocular difference in SER and AL in the center, superior, and inferior regions but not in the nasal or temporal regions. The interocular differences of the vascular density and the flow voids of the choriocapillaris were not correlated with the interocular difference of SER and AL.

Conclusions
The choroidal thickness is thinner in the more myopic eyes. The flow void is increased, and the vascular density of the choriocapillaris is reduced in the more myopic eyes of children with anisometropia exceeding 1.50 D.
Nonlinear reconfiguration of network edges, topology and information content during an artificial learning task
Authors: Shine James M. , Li Mike , Koyejo Oluwasanmi , Fulcher Ben , Lizier Joseph T. 
Published: December 2021

AbstractHere, we combine network neuroscience and machine learning to reveal connections between the brain’s network structure and the emerging network structure of an artificial neural network. Specifically, we train a shallow, feedforward neural network to classify hand-written digits and then used a combination of systems neuroscience and information-theoretic tools to perform ‘virtual brain analytics’ on the resultant edge weights and activity patterns of each node. We identify three distinct phases of network reconfiguration across learning, each of which are characterized by unique topological and information-theoretic signatures. Each phase involves aligning the connections of the neural network with patterns of information contained in the input dataset or preceding layers (as relevant). We also observe a process of low-dimensional category separation in the network as a function of learning. Our results offer a systems-level perspective of how artificial neural networks function—in terms of multi-stage reorganization of edge weights and activity patterns to effectively exploit the information content of input data during edge-weight training—while simultaneously enriching our understanding of the methods used by systems neuroscience.
A case of improvement of clozapine-induced low leukocyte counts by adenine, cepharanthin and ninjin-yoei-to in a patient with treatment-resistant schizophrenia
Authors: Kamei Shintaro , Tanaka Ryota , Hirakawa Hirofumi , Iwao Motoshi , Kawanaka Rikako , Tatsuta Ryosuke , Terao Takeshi , Itoh Hiroki 
Published: December 2021

Abstract
Background
Although clozapine is the optimal drug for patients with treatment-resistant schizophrenia, the drug has harmful adverse effects such as leukopenia. Adenine and cepharanthine are known to be effective for radiation- or drug-induced leukopenia. Furthermore, ninjin-yoei-to, a Chinese herbal medicine, augments the production of granulocyte-macrophage colony-stimulating factor. Thus, these drugs may be useful for clozapine-induced leukopenia.

Case presentation
A 21 years-old woman with schizophrenia was hospitalized for initiation of clozapine treatment. Despite concomitant use of adenine, cepharanthine, and lithium carbonate having activities of increasing leukocytes, a decrease in leukocyte counts occurred after the initiation of clozapine. Additional administration of ninjin-yoei-to increased leukocyte counts, which prevented the development of leukopenia.

Conclusions
This is the first case that concomitant use of adenine, cepharanthin, and ninjin-yoei-to exhibited the effectiveness of reversing the decrease in leukocytes caused by clozapine. Monitoring leukocyte counts and preventing leukopenia are essential for successful treatment with clozapine for refractory schizophrenia. These medicines may be a potential option for preventing clozapine-induced leukopenia.
Administration of zinc to preterm infants with hypozincemia does not reduce serum copper concentrations in most cases: a single-center retrospective observational study
Authors: Ito Toshikazu , Uenoyama Kazuya , Kobayashi Kazuhiro , Kakumoto Mikio , Mizumoto Hiroshi , Katsura Toshiya , Onoue Masahide 
Published: December 2021

Abstract
Background
Zinc is an essential trace element involved in various physiological functions. In Japan, zinc acetate dihydrate is administered to neonates and infants with hypozincemia. Since serum copper concentrations are reduced by the administration of zinc, we retrospectively investigated changes in serum zinc and copper concentrations in preterm infants with hypozincemia receiving zinc acetate dihydrate.

Methods
Sixty-three preterm infants were included in the present study. Serum zinc and copper concentrations, doses, and other clinical characteristics were retrieved from electronic medical records.

Results
The medians and interquartile ranges of the dosage and duration of zinc acetate dihydrate were 2.1 (1.8–2.5) mg/kg/day and 12.0 (10.0–13.0) days, respectively. Its administration increased serum zinc concentrations in 39 patients (61.9%) and to more than 70 μg/dL in 16 patients (25.4%). The group with a serum zinc concentration of 70 μg/dL or higher after administration had a significantly higher zinc dose of 2.5 mg/kg/day than the group with a serum zinc concentration of less than 70 μg/dL. Serum copper concentrations did not decrease in 44 patients (69.8%). In the group with a decreased serum copper concentration, postmenstrual age and body weight were significantly lower, while serum zinc concentrations were significantly higher at the start of administration.

Conclusion
The present results showed that when zinc acetate dihydrate was administered to preterm infants with hypozincemia, it was possible to increase serum zinc concentrations without decreasing serum copper concentrations in many cases. However, caution may be required when administering zinc to preterm infants with a lower postmenstrual age or milder hypozincemia because serum copper concentrations may decrease.
Nutritional and immunization status of under-five children of India and Bangladesh
Authors: Banerjee Sreeparna , SubirBiswas  , Roy Shimul , Pal Manoranjan , Hossain Md. Golam , Bharati Premananda 
Published: December 2021

Abstract
Background
The nutritional and immunization status of children can play an important role in determining their future health status of a particular country. The aim of the present study is to investigate the nutritional and immunization status of under-five children in India and Bangladesh, and to find the difference in the status between these two countries.

Methods
We have used the National Family Health Survey data, 2015–2016 of India and Bangladesh Demographic Health Survey, 2017–2018 datasets. The sample sizes are 222,418, among them 8759 and 8759 children for India and Bangladesh respectively. The nutritional status of under-five children is measured by standard anthropometric indicators of height-for-age (HAZ) and weight-for-age (WAZ). Regarding child immunization status, only BCG, DPT, polio and measles vaccinations are considered for the present study. Multiple binary logistic model has been used for analysing the data.

Results
This study reveals that the prevalence of stunting and underweight of under-five children in India are higher than Bangladeshi children. Secondary and higher educated mothers are more likely of having normal HAZ and WAZ children than up to primary educated mothers for both countries. Chances of having normal HAZ and WAZ are higher among non-poor category for both countries. The present study also shows that immunization status of Bangladeshi children is better than Indian children except measles. Religion of mother also shows influence on immunization status of children in India whereas Bangladesh shows no significant results regarding religion. Mother’s educational attainment and wealth index show influence on immunization status among children for both countries.

Conclusions
The study concludes that a remarkable number of under-five children are suffering from under nutrition for both countries, however Bangladeshi children have better nutritional and immunization status compared to Indian children. Higher wealth index, better educational attainment and lower unemployment of Bangladeshi mothers may be the causes for better nutritional and immunization status of children. Mother’s socio-economic factors have significant impact on determining the child’s health status. Our findings can help to government of Indian and Bangladesh for taking health policy to improve under-five children nutritional and immunization status.
Identification of crucial genes involved in pathogenesis of regional weakening of the aortic wall
Authors: Zu Hong Lin , Liu Hong Wei , Wang Hai Yang 
Published: December 2021

Abstract
Background
The diameter of the abdominal aortic aneurysm (AAA) is the most commonly used parameter for the prediction of occurrence of AAA rupture. However, the most vulnerable region of the aortic wall may be different from the most dilated region of AAA under pressure. The present study is the first to use weighted gene coexpression network analysis (WGCNA) to detect the coexpressed genes that result in regional weakening of the aortic wall.

Methods
The GSE165470 raw microarray dataset was used in the present study. Differentially expressed genes (DEGs) were filtered using the “limma” R package. DEGs were assessed by Gene Ontology biological process (GO-BP) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. WGCNA was used to construct the coexpression networks in the samples with regional weakening of the AAA wall and in the control group to detect the gene modules. The hub genes were defined in the significant functional modules, and a hub differentially expressed gene (hDEG) coexpression network was constructed with the highest confidence based on protein–protein interactions (PPIs). Molecular compound detection (MCODE) was used to identify crucial genes in the hDEG coexpression network. Crucial genes in the hDEG coexpression network were validated using the GSE7084 and GSE57691 microarray gene expression datasets.

Result
A total of 350 DEGs were identified, including 62 upregulated and 288 downregulated DEGs. The pathways were involved in immune responses, vascular smooth muscle contraction and cell–matrix adhesion of DEGs in the samples with regional weakening in AAA. Antiquewhite3 was the most significant module and was used to identify downregulated hDEGs based on the result of the most significant modules negatively related to the trait of weakened aneurysm walls. Seven crucial genes were identified and validated: ACTG2, CALD1, LMOD1, MYH11, MYL9, MYLK, and TPM2. These crucial genes were associated with the mechanisms of AAA progression.

Conclusion
We identified crucial genes that may play a significant role in weakening of the AAA wall and may be potential targets for medical therapies and diagnostic biomarkers. Further studies are required to more comprehensively elucidate the functions of crucial genes in the pathogenesis of regional weakening in AAA.