bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)
Authors: Radchenko E. E. , Alpatieva N. V. , Karabitsina Yu. I. , Ryazanova M. K. , Kuznetsova E. B. , Romanova O. I. , Anisimova I. N. 
Published: December 2021

Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.
Lisbeth Salander, una histeria actual
Authors: Bafico Jorge 
Published: December 2021

La trilogía literaria Millennium en la que se basaron los films suecos Los hombres que no amaban a las mujeres, La chica que soñaba con una cerilla y un bidón de gasolina y La reina en el palacio de las corrientes de aire, tienen como protagonista a Lisbeth Salander, personaje que permite trabajar conceptos psicoanalíticos de la histeria, estructura clínica que no sólo funciona como un ordenador que agrupa distintos fenómenos en una misma lógica y permite diferenciarlos de las perversiones y las psicosis, sino que remite a la modalidad por la que se transmite el deseo. Una estructura que acompañó, con la mutación de su sintomatología, los cambios sociales de la historia de la humanidad.
Simpson Study
Authors: Battisti Omar 
Published: December 2021

Este artículo argumenta cómo Los Simpson son un ejemplo particular de la cultura estadounidense, es decir, que destaca una relación diferente de los estadounidenses con la cultura. Homero y Marge Simpson no son una pareja ideal, un modelo a seguir, sino que protagonizan toda una serie de fracasos. Sin embargo, no dejan de estar juntos. Esto los convierte sobre todo en un hombre y una mujer que luchan con su relación imposible. Es precisamente en esta centralidad de lo imposible donde se puede encontrar una relación diferente con la cultura. Algo que se diferencia radicalmente de todo lo que preocupa a los estudios de género. La propuesta consiste justamente en considerar a Los Simpson como una obra que no cultiva la utopía de la relación sexual.
PERHITUNGAN HARGA POKOK PRODUKSI DAN MANAJEMEN PEMASARAN PADA USAHA RUMAHAN
Authors: Munthe Inge Lengga Sari , Sari Rizki Yuli 
Published: December 2021

Tujuan kegiatan ini agar dapat meningkatkan pengetahuan dan pemahaman mitra tentang klasifikasi biaya dan perhitungan harga pokokproduksi serta manajemen pemasaran. Pelaku bisnis ada di bidang produksi maupun non produksi, di bidang produksi berarti pelaku bisnis membuat produk atau barang dari bahan mentah menjadi barang jadi, sedangkan di bidang non produksi pelaku bisnis menawarkan jasa. Peluang usaha rumahan yang banyak dilakukan antara lain usaha kuliner, usaha laundry dan usaha salon. Usaha rumahan membutuhkan modal baik itu modal materi atau pun non materi. Metode pengabdian ini dilakukan dengan cara sosialisasi . Tim membantu melakukan perhitungan harga pokok produksi untuk menentukan harga produk dengan cara mengklasifikasi biaya ke dalam biaya produksi dan biaya non produksi dan mensosialisasikan metode pemasaran produk. Setelah diberikan pelatihan para peserta mengakui mereka memiliki kemampuan dan keterampilan yang memadai dalam membuat pencatatan perhitungan biaya produksi. Para peserta berupaya akan melakukan pencatatan untuk setiap aktivitas produksi yang mereka lakukan. Dalam hal lain, para peserta mendapatkan kemampuan lain yaitu membuat kegiatan promosi produk yang bersifat penjualan online di media sosial. Upaya tersebut memberikan manfaat perluasan jangkauan promosi produk yang harapannya dapat meningkatkan penjualan produk.
Alpha-lipoic acid did not affect lipid profile and blood pressure in critically ill patients: A randomized, double-blind clinical trial
Authors: Mohamadi Azam , Moravejolahkami Amir Reza , Askari Gholamreza , Tarrahi Mohammad Javad , Hajiesmaeili Mohammadreza , Bahreini-Esfahani Nimah 
Published: December 2021

BACKGROUND: Some researchers suggested a positive effect of Alpha-Lipoic Acid (ALA) on Blood Pressure (BP) and lipid profile. OBJECTIVE: The aim of this study was to determine the efficacy of ALA for improving BP and lipid profile in critically ill patients. METHODS: In the present study, eighty patients (aged 18–80 years old) were randomly assigned to receive daily either ALA (600 mg/day) or placebo for eight weeks. Systolic BP (SBP), Diastolic BP (DBP), Triglyceride (TG), Total cholesterol (TC), Low Density Lipoprotein (LDL), and High Density Lipoprotein (HDL) were measured before and after intervention. RESULTS: LDL decreased significantly in the ALA group (110.6±34.9 vs. 107.9±33.4 mg/dl; P < 0.05). There was also a significant difference in terms of HDL in both groups (Δ= +1.8 vs. Δ= –0.3 mg/dl; P < 0.05). A marginal non-significant reduction was also observed for SBP. There were no significant differences in other variables between the two groups. CONCLUSIONS: ALA supplementation did not improve the BP and some serum lipid markers in critically ill patients. Larger and longer trials will be needed to determine efficacy of ALA.
Condylomata Acuminata in Adolescent Girl 19th Years Old Successful with Multimodality Therapy and Combination with HPV Vaccination: A Case Report
Authors: Julia Dwi Sabtika , Qaira Anum  , Rina Gustia  
Published: December 2021

Background: Condylomata acuminata (KA) or better known as genital warts disease is a genital area infectious disease caused by Human papilloma virus (HPV). The highest prevalence infection of the condylomata acuminata occurs during active sexual periods, namely age 17-33 years, with the peak occurring at the age of 20-24 years. Currently HPV 16 and 18 are known to cause malignancy in the genital and anogenital region including cervical cancer and anal cancer, while HPV 6 and 11 are the cause of 90% of condylomata acuminata. HPV is linked to the findings of 500,000 new cases of cervical cancer and 250,000 deaths from cervical cancer each year worldwide.1
Case report: One case of condylomata acuminata in a 19-year-old adolescent girl with a major complaint of warts around her genitals that felt itchy and gradually getting bigger since 2 weeks ago. Patient was a student and unmarried. History of sexual contact with men with genito-genital without using condoms since 5 months ago. Venereological state : on the vulva and perineal there were multiple vegetations with verucose surfaces with the largest size 0.8 cm x 0.5 cm x 0.1 cm and the smallest size 0.2 cm x 0.2 cm x 0.1 cm. Acetowhite examination result was positive and PCR examination results found positive HPV types 6,11,16 and 18.
Discussion: Patients are diagnosed to condylomata acuminata with a history of free sex at an early age. The condylomata acuminata experienced by patients was type 6,11,16 and 18. These types of 16 and 18 HPV are know to cause malignancy in the genital and anogenital region including cervical cancer and anal cancer.
Comparison of Germ Patterns and Antimicrobial Susceptibility in Chronic Suppurative Otitis Media with Cholesteatoma and without Cholesteatoma in Dr. Mohammad Hoesin Hospital.
Authors: Hifni Ahmad , Rian Hasni  , Fiona Widyasari  , Abla Ghanie  , Erial Bahar  
Published: December 2021

Introduction: Chronic suppurative otitis media (CSOM) is a chronic inflammation of the middle ear and mastoid cavity. It is necessary to identify bacterial patterns and antibiotics susceptibility in CSOM. Appropriate administration of antibiotics is essential to treat infection and prevent widespread resistance.
Objective: The aim of this study is to compare the pattern of germs and antibiotic susceptibility between CSOM with cholesteatoma and without cholesteatoma.
Methods: Observational study with cross-sectional design. Data collection was carried out using medical record data on 102 subjects who underwent middle ear swab and bacterial culture from January to December 2019.
Results: From 102 subjects and 122 bacterial culture results. it was found that Pseudomonas aeruginosa (36.1%) was one of the most common organisms to cause CSOM. followed by Staphylococcus haemolyticus. Staphylococcus epidermidis and Staphylococcus aureus. There was a significant difference between the germ pattern and the type of CSOM (p = 0.002). Amikacin. meropenem. gentamicin. cefepime. ciprofloxacin and ceftazidime were found to be the most effective antibiotics for Pseudomonas aeruginosa. The rate of resistance to tigecycline and cefazoline were highest. Age. sex. nutritional status and comorbidities were not factors that significantly influenced the incidence of cholesteatoma in CSOM.
Conclusion: There is a significant difference between the germ pattern in both CSOM groups. Patients that show Gram negative have a significant effect on the incidence of CSOM with cholesteatoma.
Lung Abscess Carcinomatous
Authors: Puspita Upi , Fauzar  , Roza Kurniati  
Published: December 2021

Introduction: Pulmonary malignancies may easily be overlooked and valuable time may be lost. Lung cancer is sometimes diagnosed as a tuberculous cavity or an abscess. Abscess formation can appear in several different ways. Carcinoma that occurs in medium-sized bronchi causes partial bronchial obstruction, atelectasis, and infection due to retention. Inflammation can progress to damage to lung tissue, resulting the formation of multiple suppurative foci or more localized lung abscess. The link between lung abscess and lung cancer has been known, but the presence of malignancy in lung abscesses often undiagnosed. Obstruction from lung cancer can predispose to the development of a lung abscess. Case of a 54 year old man with increased pain at the right chest when breathing in since two months. On physical examination, it was found decreased of fremitus at the right hemithorax, deafness and decreased breath sounds as high as the II - V thoracic right hemithorax. Thorax CT-scan showed a round, homogeneous (HU: 17-30), with a cavity-like image with air fluid level, size: 7,88 cm x 8,2 cm x 9,29 cm and honey comb appearance around it. On the examination of TTNA (Transthoracic Needle Aspiration) results obtained Squamous Cell Carcinoma. A lobectomy is planned for the patient.
Conclusion: We reported a rare case, a 54 year old male patient, with the diagnosis of Carcinomatous Lung Abscess. This case report was prepared with the aim of increasing awareness of malignancy in patients with a clinical presentation of abscesses, especially in old age.
Alpenprojekt
Authors: Camargo Marina 
Published: December 2021

Alpenprojekt videos register the action of cutting the skyline in the alpine mountains. The footage was taken at different sites in the Alps. The cutouts evoke the European tradition from the 18th century to depict portraits with scissors and paper. A deliberate intent to apprehend the landscape within a unique line in a reduced dimension is the main issue in Alpenprojekt I and II. To react in the face of this specific landscape as an effort to embrace what is not controllable became a fundamental issue in the Alpenprojekt series of works. Alpenprojekt began with artistic research related to the southern German region closely connected with its physical landscape. Its representation was then perceived as a memory heritage of historical facts, either forgotten or intentionally lost. The entire project is called Trilogy of the Mountains, and it is related to memory and history. Trilogy of the Mountains comprises three phases: Alpenprojekt, based on the alpine landscape; the second one approaches Beckton Alps, an artificial mountain in east London; and the third part is related to artificial mountains made with war debris in Germany. Each piece of the Trilogy comprises a series of works. The project was initially developed based on landscapes where the notion of Romanticism is still present. Then the project was set toward the post-industrialization period—and finally related to reshaping the topography in Germany after WWII. In Trilogy of Mountains, the tension between natural and artificial is a central issue, being rather complementary than the opposite.
Naturaleza jurídica de las ¨sociedades civiles y mercantiles¨ y su relación con la práctica notarial
Authors: Arroba López David Alejandro 
Published: December 2021

En el Ecuador, coexisten un sin número de sociedades de distinta naturaleza, entre las que conviene mencionar a las sociedades civiles, sociedades mercantiles, y aquellas sociedades llamadas ¨de hecho¨. Pero además de las referidas, existe un tipo de sociedad que en la práctica se está constituyendo, me refiero con esto a las sociedades ¨civiles y mercantiles¨ mismas que conforme se analizará a lo largo de este artículo no pueden ser consideradas como si se tratase de un tipo societario, ya que las sociedades o bien son civiles o, bien mercantiles, y que la atribución contenida en el numeral 29, del artículo 18, de la ley notarial -que es el sustento legal en el que se está basando la creación de estas sociedades- lo que pretendía era eliminar el requisito de aprobación judicial existente para las compañías mercantiles en nombre colectivo y en comandita simple, transfiriendo esa facultad al servicio notarial, más no crear una figura jurídica inexistente como lo es la llamada ¨sociedad civil y mercantil¨. Objetivo.- el objetivo del presente trabajo es demostrar a través del análisis de varios casos, cómo mediante la interpretación del artículo 18, numeral 29, de la ley Notarial, se están constituyendo erróneamente sociedades ¨civiles y mercantiles¨ como si fuesen una especie de compañía. Metodología.- En el presente trabajo de investigación se ha aplicado los métodos: inductivo e histórico lógico. Aportes.- El presente trabajo busca aportar en el debate jurídico societario ecuatoriano, al criticar la interpretación que se está dando de una norma que equivocadamente ha permitido la constitución de las llamadas ¨sociedades civiles y mercantiles¨, que conforme se analizará no tiene un sustento legal.
Development of Learning Unit Book based on Insect Inventory Results of Amorphophallus titanum as an Effort to Foster Students’ Concern to the Environment
Authors: Harianto Harianto , Karyadi Bhakti , Wardana Rendy W. 
Published: December 2021

Natural Science subject learning materials emphasize the observation of natural phenomena and their application in daily life. This research aims to foster students' concern for the environment through a learning unit book on the interaction of living things. The type of research was a "research and development method" with the design of 4-D model development research. The learning unit book was made based on the results of insect inventory on Amorphophallus titanum. The learning unit book was presented to the 8th-grade students of SMP Negeri Rejang Lebong, Bengkulu. The subject in this study was class VIII B on a limited trial. The subject was selected using the purposive sampling method. The data from the study was obtained by providing a questionnaire on environmental concerns. Analysis used test-n-Gain, and small-scale test results of environmental concern found an average score of 77.24 that are well categorized.
The Implementation of Strain Liquefaction Learning Package to Cultivate the Critical Thinking of High School Students
Authors: Pirnandes Lubis , Karyadi Bhakti , Farid M. 
Published: December 2021

This study aims to explain the potential of liquefaction in Padang Lekat Village and Pasar Ujung Village Kepahiang District, explaining the feasibility of developed learning packages, and explaining students' critical thinking skills after learning the wave material through the learning package "Strain Liquidity Learning". The type of study used was research and development which aims to develop digital-based physics learning packages that are integrated with disaster mitigation education on the basic competencies of waves in high school education units. This research was conducted using ADDIE development research procedures. The results of research on the analysis of liquefaction potential based on microtremor data using the Ground Shear Strain method, zoning indication of liquefaction potential due to geological conditions and seismic potential, generally Padang Lekat Village and Pasar Ujung Kepahiang Village are areas that have no potential to experience liquefaction. However, there are a number of locations indicated by potential liquefaction, although not significantly. Learning Package Book "Learning Liquid Strain" compiled based on the analysis of liquefaction potential based on expert validation is appropriate to be used as a learning medium for seismic and liquefaction waves. In its application with distance learning, the compiled learning package book has been able to improve critical thinking skills in students even though the N-gain improvement obtained is still in the low and medium categories.
NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells
Authors: Wang Yan , Li Naijian , Wang Yingfeng , Zheng Guobing , An Jing , Liu Chang , Wang Yajie , Liu Qicai 
Published: December 2021

Objective: Peroxisome proliferator-activated receptor gamma (PPARγ) has an anti-proliferation effect on pulmonary arterial smooth muscle cells (PASMCs) via the transient receptor potential channel (TRPC) and protects against pulmonary artery hypertension (PAH), whereas nuclear factor-kappa B (NF-κB) has pro-proliferation and pro-inflammation effects, which contributes to PAH. However, the association between them in PAH pathology remains unclear. Therefore, this study aimed to investigate this association and the mechanisms underlying TRPC1/6 signaling-mediated PAH.Methods: Human pulmonary arterial smooth muscle cells (hPASMCs) were transfected with p65 overexpressing (pcDNA-p65) and interfering plasmids (shp65) and incubated in normal and hypoxic conditions (4% O2 and 72 h). The effects of hypoxia and p65 expression on cell proliferation, invasion, apoptosis, [Ca2+]i, PPARγ, and TRPC1/6 expression were determined using Cell Counting Kit-8 (CCK-8), Transwell, Annexin V/PI, Fura-2/AM, and western blotting, respectively. In addition, the binding of p65 or PPARγ proteins to the TRPC6 promoter was validated using a dual-luciferase report assay, chromatin-immunoprecipitation-polymerase chain reaction (ChIP-PCR), and electrophoretic mobility shift assay (EMSA).Results: Hypoxia inhibited hPASMC apoptosis and promoted cell proliferation and invasion. Furthermore, it increased [Ca2+]i and the expression of TRPC1/6, p65, and Bcl-2 proteins. Moreover, pcDNA-p65 had similar effects on hypoxia treatment by increasing TRPC1/6 expression, [Ca2+]i, hPASMC proliferation, and invasion. The dual-luciferase report and ChIP-PCR assays revealed three p65 binding sites and two PPARγ binding sites on the promoter region of TRPC6. In addition, hypoxia treatment and shPPARγ promoted the binding of p65 to the TRPC6 promoter, whereas shp65 promoted the binding of PPARγ to the TRPC6 promoter.Conclusion: Competitive binding of NF-κB p65 and PPARγ to TRPC6 produced an anti-PAH effect.
Early Thalamic Injury After Resuscitation From Severe Asphyxial Cardiac Arrest in Developing Rats
Authors: Ton Hoai T. , Raffensperger Katherine , Shoykhet Michael 
Published: December 2021

Children who survive cardiac arrest often develop debilitating sensorimotor and cognitive deficits. In animal models of cardiac arrest, delayed neuronal death in the hippocampal CA1 region has served as a fruitful paradigm for investigating mechanisms of injury and neuroprotection. Cardiac arrest in humans, however, is more prolonged than in most experimental models. Consequently, neurologic deficits in cardiac arrest survivors arise from injury not solely to CA1 but to multiple vulnerable brain structures. Here, we develop a rat model of prolonged pediatric asphyxial cardiac arrest and resuscitation, which better approximates arrest characteristics and injury severity in children. Using this model, we characterize features of microglial activation and neuronal degeneration in the thalamus 24 h after resuscitation from 11 and 12 min long cardiac arrest. In addition, we test the effect of mild hypothermia to 34°C for 8 h after 12.5 min of arrest. Microglial activation and neuronal degeneration are most prominent in the thalamic Reticular Nucleus (nRT). The severity of injury increases with increasing arrest duration, leading to frank loss of nRT neurons at longer arrest times. Hypothermia does not prevent nRT injury. Interestingly, injury occurs selectively in intermediate and posterior nRT segments while sparing the anterior segment. Since all nRT segments consist exclusively of GABA-ergic neurons, we asked if GABA-ergic neurons in general are more susceptible to hypoxic-ischemic injury. Surprisingly, cortical GABA-ergic neurons, like their counterparts in the anterior nRT segment, do not degenerate in this model. Hence, we propose that GABA-ergic identity alone is not sufficient to explain selective vulnerability of intermediate and posterior nRT neurons to hypoxic-ischemic injury after cardiac arrest and resuscitation. Our current findings align the animal model of pediatric cardiac arrest with human data and suggest novel mechanisms of selective vulnerability to hypoxic-ischemic injury among thalamic GABA-ergic neurons.