Antihyperlipidemic and Antiobesity Potential of Aquilaria agallocha and Borago officinalis in Fixed-Dose Combination; A Contingent Probe with Atorvastatin and Orlistat
Authors: Oraon Rohima , Mahmood Tarique Ansari , Shamim Arshiya , Ahsan Farogh , Shariq Mohammad , Parveen Saba , Alam Mehtab 
Published: March 2021

Aims:
Antihyperlipidemic and antiobesity potential of a fixed dose combination of Aquilaria
agallocha & Borago officinalis along with its comparative evaluation with Atorvastatin & Orlistat.

Background:
Hyperlipidemia and obesity are common and challenging health problems throughout
the world. These are the conditions in which increased lipids level in blood are the risk factors
for atherosclerosis, coronary artery disease, and cerebral vascular diseases. In the present study, the
fixed-dose combination of absolute ethanolic extract of Aquilaria agallocha and aqueous extract of
Borago officinalis was examined for its antihyperlipidemic effect and antiobesity activity in the
Sprague Dawley rats.

Objective:
A suitable & established experimental model of hyperlipidemia and obesity based on literature
survey has been selected. Comparative evaluation of fixed dose combination with selected
standard drug against the established model. Compilation of results and statistical analysis.

Methods:
Drugs and Chemicals: Atorvastatin solution, Orlistat solution, Formalin solution Animals:
sprague dawley rats of 150-200 g of either sex were used for the study. Animals were habituated
to laboratory conditions for 48 hours prior to experimental protocol to minimize if any of nonspecific
stress. All the studies conducted were approve by the Institutional Animal Ethical Committee
(IAEC) of Faculty of Pharmacy, Integral University Lucknow (Reg. no. 1213/PO/Re/S/08/CPCSEA,
5th June 2008) having approval no. IU/IAEC/18/32. High fat diet induced Dyslipidemia and
obesity: Baseline characteristics of obesity and hyperlipedimia induced by high fat diet in Sprague
dawley rats were maintained on normal pellet diet (NPD) for one week before the commencement
of experiment. After one week rats were randomly assigned into normal and obese group and fed
with NPD and High fat diet and libitum respectively for one week.The food intake, body weight
gain ,and lipid profile were carried out on day 8 of dietary manipulation to assess the baseline characteristics
of obesity and hyperlipidemia induced by high fat diet in Sprague dawley rats.

Results:
The result of the study shows that the ethanolic extract of the FDC of aquilaria agallocha
& borago officinalis has significant anti-hyperlipidemic and anti-obesity activity against High- fat
diet-induced hyperlipidemia and obesity compared to atorvastatin (10 mg/kg) and orlistat (pure
drug 10mg/kg).

Conclusion:
The present study shows that pretreatment with ethanolic extract of FDC of Aquilaria
agallocha & Borago officinalis significantly prevented from hyperlipidemia and obesity and decreased
the levels of diagnostic marker enzymes significantly. At low dose (Borago officinalis 100
mg/kg + Aquilaria agallocha 250 mg/kg b.w.) ) ethanolic extract of FDC of Aquilaria agallocha &
Borago officinalis showed moderate protection against Hyperlipidemia and Obesity. At high dose
(Borago officinalis 200 mg/kg + Aquilaria agallocha 500 mg/kg b.w.) ethanolic extract of FDC of
Aquilaria agallocha & Borago officinalis showed marked protection against High fat diet induced
hyperlipidemia and obesity as well as by biochemical estimations of Lipid profile compare to the
standard Atorvastatin(10mg/kg) and Orlistat(10mg/kg). Thus it concludes that ethanolic extract of
FDC of Aquilaria agallocha & Borago officinalis possesses potent against high fat diet induced hyperlipidemia
and obesity.