bluuebunny/crossref_metadata_2025_split huggingface dataset:
PPARβ/δ Priming Enhances the Anti-Apoptotic and Therapeutic Properties of Mesenchymal Stromal Cells in Myocardial Ischemia-Reperfusion Injury
Authors: Sarre Charlotte , Lopez Rafael Contreras , Nerpernpisooth Nitirut , Barrere Christian , Bahraoui Sarah , Terraza Claudia , Tejedor Gautier , Vincent Anne , Luz-Crawford Patricia , Kongpol Kantapich , Kumphune Sarawut , Piot Christophe , Nargeot Joel , JORGENSEN Christian , Djouad Farida , Barrere-Lemaire Stéphanie 
Published: December 2021

Abstract
Background: Mesenchymal Stromal Cells (MSC) have been widely used for their therapeutic properties in many clinical applications including myocardial infarction. Despite promising preclinical results and evidences of safety and efficacy in phases I/ II, inconsistencies in phase III trials have been reported. In a previous study, we have shown using MSC derived from the bone marrow of PPARβ/δ (Peroxisome proliferator-activated receptors β/δ) knockout mice that the acute cardioprotective properties of MSC during the first hour of reperfusion are PPARβ/δ-dependent but not related to the anti-inflammatory effect of MSC. However, the role of the modulation of PPARβ/δ expression on MSC cardioprotective and anti-apoptotic properties has never been investigated. Objectives: The aim of this study was to investigate the role of PPARβ/δ modulation (inhibition or activation) in MSC therapeutic properties in vitro and ex vivo in an experimental model of myocardial infarction.Methods and results: Naïve MSC and MSC pharmacologically activated or inhibited for PPARβ/δ were challenged with H202. Through specific DNA fragmentation quantification and qRT-PCR experiments, we evidenced in vitro an increased resistance to oxidative stress in MSC pre-treated by the PPARβ/δ agonist GW0742 versus naïve MSC. In addition, PPARβ/δ-priming allowed to reveal the anti-apoptotic effect of MSC on co-cultured cardiomyocytes. When injected during reperfusion in an ex vivo heart model of myocardial infarction, PPARβ/δ-primed MSC at a dose of 3.75x105 MSC/heart provided the same cardioprotective efficiency than 7.5x105 naïve MSC, identified as the optimal dose in our model. These enhanced short-term cardioprotective effects were associated with an increase in both anti-apoptotic effects and the number of MSC detected in the left ventricular wall at 1 hour of reperfusion. By contrast, inhibition of PPARβ/δ before their administration in post-ischemic hearts during reperfusion decreased their cardioprotective effects. Conclusion: Altogether these results revealed that PPARβ/δ-primed MSC exhibit an increased resistance to oxidative stress and enhanced anti-apoptotic properties on cardiac cells in vitro. PPARβ/δ-priming appears as an innovative strategy to enhance the cardioprotective effects of MSC and to decrease the injected doses. These results could be of major interest to improve MSC efficacy for the cardioprotection of injured myocardium in AMI patients.
Plant extract as gas adsorbent to reduce smoke toxicants of cigarette
Authors: Yonggang Liu 
Published: December 2021

Abstract
Cigarette smoke contains thousands of chemicals including many known toxicants and annually leads to millions of deaths worldwide. To reduce the harms of cigarette, plant extracts were applied to adsorb smoke toxicants of cigarette. Results showed that platycladus orientalis leaf extract and mulberry fruit extract particles filled into cigarette cellulose acetate filter can significantly reduce 15 major cigarette smoke toxicants emission including hydrogen cyanide (HCN), benzo[α] pyrene (B[a]P), formaldehyde, crotonaldehyde, 2-butanone, P-hydroquinone, M-dihydroxybenzene, catechol, phenol, M-P-cresol, O-cresol, N'-nitrosonornicotine (NNN), 4-methylnitrosamino-l-3-pyridyl- butanone (NNK), (R,S)-N-nitrosoanatabine (NAT) and (R,S)-N-nitrosoanabasine (NAB) by 11.90% to 60.42% (P<0.01). Platycladus orientalis leaf extract particles added in the outer cigarette filter also can adsorb other 125 kinds of chemicals most of which are harmful. Our results also indicated that plant extract has extensive gas adsorption characteristics and different plant extracts displayed different adsorption capacity to different toxicants. The adsorption capacities of five randomly selected plant extracts are all significantly higher than that of activated carbon（P<0.01). These findings suggest that plant extracts are excellent cigarette smoke adsorbents.
Assessment of Knowledge, Perception, Attitude, Risk Factors Prevention and Treatment Options of Cancer Among Natives in Elgon Sub-Region, Uganda
Authors: Kudamba Ali , Jamil E. Ssenku , Okurut Shaban A. , Kafero Hussein M. , Nsubuga Hakim , Walusansa Abdul 
Published: December 2021

Abstract
Background

Globally, cancer is one of the leading cause of morbidity and mortality and most cancers are due to infections and so, are preventable. Studies have shown that cancer prevention has been possible through intensified healthcare education but such information is poorly documented in Elgon sub-region. Therefore, our study was aimed at assessing cancer indigenous knowledge among natives in Elgon sub-region.
Method

Mixed methods research design were adopted. A total of 73 participants, selected through snowball sampling technique were involved. Data collection was done through pretested questionnaires. MedCalc version, 20.008 was used for data analysis and results were presented in tables and figures.
Result

Majority of the study participants were males (58%), aged between 46 – 55 years (58%), Ugandans (90%) and married (67%). Most of them had inadequate knowledge about cancer (p<0.05) and highlighted sores that slightly heal at any body parts as well as blood in faeces as putative predictors of cancer infection (p<0.005). A total of nine cancer types were documented and cervical was the most prevalent (p<0.0001). Smoking was the most pronounced cancer associated risk factor (p<0.0001) and avoiding smoking was perceived as the major prevention option (p<0.0001). No cancer treatment options in cancer treatment cited in this area (p<0.172).
Conclusion

The natives had limited knowledge and poor perception of cancer due low literacy level. Therefore, there is need to intensify on cancer health education programs through the word of mouths and radio talk shows. The plant medicinal plant used in cancer treatment needs to be documented.
The Hardware Failure and Correction Loss of Short-segment Pedicle Screw Fixation Combined With Transforaminal Interbody Fusion in Treatment of Unstable Thoracolumbar Burst Fracture: Retrospective Study in Vietnam
Authors: Nguyen Ngoc Quyen , Phan Trong Hau , Vu Van Hong 
Published: December 2021

Abstract
Background: Correction loss and hardware failure of short segment posterior pedicle screw fixation in treatment of thoracolumbar unstable burst fracture have been remaining a main concern. Several authors have introduced the procedures to solve these limitations including transforaminal interbody fusion (TIF). The purposes of this study were to evaluate the progressive kyphosis and implant failure of short-segment pedicle screw fixation combined with transforaminal interbody fusion in treatment of unstable thoracolumbar burst fracture.Methods: The retrospective study were enrolled in the patients with isolated unstable thoracolumbar burst fractures, Denis type IIB who were treated by posterior short fixation with TIF between January 2013 to January 2017. Patients were followed up for a minimum of one and half year. For evaluation of correction loss, % loss of anterior vertebral body heights (%AVB), vertebral kyphotic angle (VA) and regional kyphotic angle (RA) were collected preoperatively, postoperatively and at final follow-up. The hardware failure was assessed on radiological images at last follow-up. Results: There were 36 patients who met the inclusion criteria with a mean follow-up duration of 53 months. The mean correction loss of %AVB, VA and, RA were 10.2%, 2.9o and 5.6o, respectively. There were 6 patients (16.7%) with hardware failure at final follow-up. Conclusions: Short-segment posterior pedicle screw fixation with TIF using bone chip graft hasn’t prevented completely the hardware failure and progressive kyphosis in treatment of unstable thoracolumbar burst fracture.
A Register-Based Study: Cough - A Frequent Phenomenon in the Adult Population
Authors: Backer Vibeke , Porsborg Andreas , Hansen Victor , Skjold Tina , Schmid Johannes Martin , Kehlet Mette , Torp-Pedersen Christian , Aasbjerg Kristian 
Published: December 2021

Abstract
BackgroundChronic cough, more than 8 weeks, can either be without co-morbidity called unexplained chronic cough (UCC) or with co-morbidity called refractory chronic cough (RCC). Using datasets from the Danish National Prescription Registry (Prescription Registry) and Danish National Patient Registry (Patient Registry) we wanted to investigate the prevalence of cough in a Nationwide registry from both primary and secondary care setting.Material and MethodsInclusion criteria were patients 18-90 years with at least one final cough diagnosis (ICD-10 DR05/DR059) in Patient registry or patients who have redeemed ≥2 prescriptions for relevant cough-medication within a 90-day harvest in the Prescription registry from 2008-2017. To validate this study’s chosen proxy on chronic cough an analysis of the Patient registry sub-population with a contact of ≥8 weeks and then final diagnosis code DR05/DR059 was also performed. The population was divided into UCC and RCC.ResultsOf the 104.216 patients from the Prescription registry, 52.727 were classified as having UCC and 51.489 were classified with RCC. From the Patient registry 34.260 were included, of whom 12.278 had UCC and 21.982 had RCC. Cough were frequently found among females between 50 and 66 years. Spirometry was performed in 69% and 57%, X-ray in 73% and 58% and asthma challenge test performed in 13% and 5% (UCC and RCC, respectively). The frequency of co-morbidities such as heart failure, rheumatologic disease, pulmonary embolism, and diabetes was < 10%. The distribution of all the included variables of patients who have had attachment to the department for 8 weeks or more, was similar as in the main group, thus fewer.ConclusionMany patients suffer from chronic cough or cough requiring medications, with or without co-morbidity; frequently found among menopausal women. Most patients had a substantial work-up performed. The high frequency and the resources consuming work-up program call for systematic coding of disease, systematic patient evaluation and more specific treatment options.The study was accepted by the Danish Data Protection Agency (ID: no. P-2019-191)
Brain Computer Interface -EEG Based Imagined Word Prediction Using Convolutional Neural Network Visual Stimuli for Speech Disability
Authors: Chinta Babu , M Moorthi 
Published: December 2021

Abstract
Background: Brain Computer Interface (BCI) is one of the fast-growing technological trends, which finds its applications in the field of the healthcare sector. In this work, 16 electrodes of Electroencephalography (EEG) placed according to the 10-20 electrode system is used to acquire the EEG signals. A BCI with EEG based imagined word prediction using Convolutional Neural Network (CNN) is modeled and trained to recognize the words imagined through the EEG brain signal, where the CNN model Alexnet and Googlenet are able to recognize the words due to visual stimuli namely, up, down, right, left and up to ten words. The performance metrics are improved with the Morlet Continuous wavelet transform applied at the pre-processing stage, with seven extracted features such as mean, standard deviation, skewness, kurtosis, bandpower, root mean square, and Shannon entropy.Results: Based on the testing, Alexnet transfer learning model performed better as compared to Googlenet transfer learning model, as it achieved an accuracy of 90.3%, recall, precision, and F1 score of 91.4%, 90%, and 90.7% respectively for seven extracted features. However, the performance metrics decreased when the number of extracted features was reduced from seven to four, to 83.8%, 84.4%, 82.9%, and 83.6% respectively.Conclusions: The Alexnet transfer learning model is selected to be the best model as compared Googlenet, as it achieved an accuracy of 90.3% and the final training option of 80 epoch, 64 batch size, scalogram pre-processing method, ratio of 80:20 training and validation set and initial learning rate of 0.0001.
Diagnostic and Prognostic Significance of Serum Tumor Markers in Patients with Lung Cancer
Authors: Chen Suipeng , Kuang Mengjiao , Wang Shishi , Huang Shirui , Li Jizhen , Zhou Teng , Wang Huiyan , Cai Yue , Yang Wei , Xie Qipeng 
Published: December 2021

Abstract
Background: Tumor markers CEA, CYFRA21-1, NSE, proGRP, and SCC-Ag were routinely used for lung cancer. The objective of this study was to evaluate the diagnostic and prognostic value of these markers.Methods: The levels of 5 serum tumor markers were measured in 255 patients with lung cancer (44 squamous cell carcinoma, 183 adenocarcinoma, 28 small cell lung cancer), 103 patients with benign lung disease, and 120 healthy controls. The relevant clinical data of the patients were collected to analyze the expression of CEA, CYFRA21-1, NSE, proGRP, and SCC-Ag in the serum of lung cancer and their correlation with prognosis.Results: The positive rates and expression levels of CEA, CYFRA21-1, NSE, proGRP, and SCC-Ag in the lung cancer group were higher than those in patients with benign lung diseases and healthy controls. CYFRA21-1 and SCC-Ag were highly expressed in lung squamous cell carcinoma, and NSE and proGRP were highly expressed in small cell lung cancer. The expression levels of CYFRA 21-1, NSE, and proGRP in small cell lung cancer were higher than those in non-small cell lung cancer. The levels of CEA, CYFARA21-1, NSE, and proGRP were linearly correlated with the occurrence of lung cancer, while the level of SCC-Ag was not significantly correlated with the occurrence of lung cancer. What’s more, the levels of CYFAR21-1 and NSE in the death group of lung cancer patients were higher than those in the survival group.Conclusion: CEA, CYFRA21-1, NSE, proGRP, and SCC-Ag played a good role in diagnosing lung cancer. CYFRA21-1 and SCC-Ag can be used as markers for the diagnosis of squamous cell carcinoma, and NSE and proGRP can be used as markers for small cell lung cancer. Meanwhile, the combined detection of CEA, CYFARA21-1, NSE, and proGRP had the highest diagnostic accuracy for lung cancer. Furthermore, CYFAR21-1 and NSE can be used to evaluate the prognosis of lung cancer patients.
The genetic origin and architecture of sex determination
Authors: De Tisham 
Published: December 2021

Abstract
Here, I demonstrate that sex determination and sexual dimorphism across tree of life are deeply
related to polyamine biochemistry in cells, especially to the synteny of genes: [SAT1-NR0B1],
[SAT2-SHBG] and DMRT1. This synteny was found to be most distinct in mammals. Further, the
common protein domain of SAT1 and SAT2 - PF00583 was shown to be present in the genome of
the last universal common ancestor (LUCA). Protein domain-domain interaction analysis of
LUCA’s genes suggests the possibility that LUCA had developed an immune defence against
viruses. This domain-domain interaction analysis is the first scientific evidence indicating that
viruses existed at least 3.5 billions years ago and probably co-existed with LUCA on early Hadean
Earth.
Benefits and Limitations of Business Process Model Notation in Modeling Patient Healthcare Trajectory: A Scoping Review Protocol
Authors: Abasse Kassim Said , Gartner Jean-Baptiste , Labbé Laurence , Landa Paolo , Paquet Catherine , Bergeron Frédéric , Lemaire Célia , Côté André 
Published: December 2021

Abstract
Introduction:

The adoption of business process model notation (BPMN) in modeling healthcare trajectory can enhance the efficiency and efficacy of healthcare organizations and ultimately improve patient outcomes while restraining costs. However, existing systematic reviews have been inconclusive regarding the effectiveness of BPMN in modeling healthcare trajectory. The aims of this scoping review are to map and aggregate existing evidence on the main benefits and limitations associated with BPMN in healthcare trajectory and highlight areas of improvement on using BPMN and its extensions in healthcare practices, which have not been systematically scoped.
Methods and Analysis

: The proposed scoping review will be performed in accordance with the methodological framework suggested by Arksey and O’Malley and further refined by Levac et al. A wide range of electronic databases and grey literature sources will be systematically searched using predefined keywords, from 2004 onwards. The review will include any study design with a focus on the application of the BPMN approach applied for optimizing healthcare trajectories (e.g., diagnostic, and therapeutic process, decision making, cost, and resources), published in either English or French. Two reviewers will independently screen titles, abstracts, and full-text articles and select studies meeting the inclusion criteria. A customized data extraction form will be used to extract data from the included studies. The results will be presented in tabular format developed iteratively by the research team.
Ethics and dissemination

: Research ethics approval is not required as exclusively secondary data will be used. Review findings will be used to advance understanding about BPMN, its extensions and its application in healthcare trajectory optimization. The review will develop recommendations about how to tailor BPMN strategies at optimizing care pathways and decision-making processes. Our findings will be disseminated in peer-reviewed journals and presentations and through discussions with relevant organizations and stakeholders.
An Elementary Proof to the Riemann Hypothesis
Authors: Hernandez-Serna Eduin 
Published: December 2021

Abstract
Let Ƥ be the set of all primes, Ψ/(n) = nIIn∈Ƥ,p|n (1 + 1/Ƥ) be the Dedekind psi function, we unconditionally show that eγ log log n > Ψ(n)/n for any n > 30, where γ if Euler's constant.
Role of salivary glycopatterns for oral microbiota associated with gastric cancer
Authors: Shu Jian , Yu Hanjie , Ren Xiameng , Wang Yan , Zhang Kun , Tang Zhen , Dang Liuyi , Chen Wentian , Li Baozhen , Li Zheng 
Published: December 2021

Abstract
Microbiota in the oral cavity plays an important role in maintaining human health. Our previous studies have revealed significant alterations of salivary glycopatterns in gastric cancer (GC) patients, but it is unclear whether these altered salivary glycopatterns can cause the dysbiosis of oral microbiota. In this study, the oral microbiome of healthy volunteers and GC patients were detected. The neoglycoproteins were then synthesized according to the altered glycopatterns in GC patients and used to explore the effects of specific glycopattern against oral microbiota. The results showed that the fucose-neoglycoproteins could reduce the adhesion and toxicity of A. segnis to oral cells, change the glycan structures of lipopolysaccharide, and enhance the immunogenicity of A. segnis. This study revealed that the changes of salivary glycopatterns in GC patients might contribute to the dysbiosis of oral microbiota, had important implications in developing new carbohydrate drugs to maintain a balanced microbiota in the oral.
A genome-wide association study to identify candidate genes for metabolic disorders in offspring by in vitro fertilization
Authors: Liu Lihong , Ha Siyao , Li Zhiling , Li MingQing 
Published: December 2021

Abstract
In vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear. We conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks. We identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, confirmed a conserved motif, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring. The impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
Teratogenic Potential of Solenstemma Argel Extract in Wistar Albino Rats
Authors: Mustafa Nazik M.E. , Satti Shahenaz , Osman Nafisa A. , A.Gameel Ahmed , El-hadiyah Tarig M. 
Published: December 2021

Abstract
The majority of people in Africa receive their basic health care through herbal treatments. Herbal medicine may negatively impact fetal development irreparably. This study examined the teratogenic potential of Solenstemma argel extract in pregnant rats. Pregnant rats were treated with Solenstemma argel from 7th to 16th day of gestation. The dosage used was 250 mg/kg, intraperitoneal.Solenstemma argel extract treated group showed fetal abnormalities appeared as body hemorrhage, limbs abnormalities and resorption of fetuses. These appears in 25% of the fetuses (P-value = 0.01) which is significantly differed from control group. Furthermore, histopathological findings of liver sections from fetuses of Solenstemma argel - treated mothers showed loose liver texture and hepatocytes hemorrhage.In this study, we conclude that the use Solenstemma argel extract during the organogenesis period in pregnant rats has the potential to cause teratogenic effects, as well as abnormalities in liver histopathology.
A Real-World Study on Myopia Control 6 Months After Withdrawal of 1% Atropine Eye Gel
Authors: Xu Yan , Chen Jun , Tang Chun , Xiang Kaidi , Cui Lipu , He Xiangui 
Published: December 2021

Abstract
Purpose: This study aimed to observe the changes in spherical equivalent and ocular axial length 6 months after withdrawal of 1% atropine eye gel.Methods: Due to COVID-19, the follow-up of patients in our optometric clinic who were undergoing myopia control treatment with a dropwise 1% atropine “5+3” regimen was interrupted. No return visit was made after the 3 months of at-home treatment, and follow-ups resumed 6 months after treatment withdrawal. The contralateral eye was not treated over the 9-month period. A total of 16 patients aged 11.5 years (average) were enrolled from November 2019 to March 2021 during the COVID-19 pandemic. The treated eyes formed a treatment group (16 eyes) and the contralateral eyes formed a control group (16 eyes). The changes in spherical equivalent, ocular axial length, and intraocular pressure (IOP) were compared between groups. Results: After 9 months, the changes in spherical equivalent were significantly less in the treatment group (0.00 ± 0.20 [D]) compared to the control group (-0.67 ± 0.25 [D]) (P<0.05). The ocular axial length changes were significantly less in the treatment group (0.00 ± 0.06 mm) compared to the control group (0.25 ± 0.11 mm) (P<0.05). There was no significant difference between the two groups for changes in IOP. Conclusions: Despite treatment withdrawal after 3 months, treatment with 1% atropine eye gel successfully controlled myopia progression in the 6 months after withdrawal, as evidence by no rebound increase in myopic spherical equivalent after the withdrawal.