bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing Mental Health Stigma in Primary Healthcare Workers in a Rural Chinese County: a Contact-based Family-centric Approach
Authors: Wang An-Li , Qiu Selinda , Zhang Tian-Ming , Deng Yuer , Ran Mao-Sheng , Wong Yin-Ling Irene 
Published: August 2021

Abstract
Background: Mental health-related stigma is a key deterrent to help-seeking in people with mental illness. However, effective stigma reduction interventions are sparse, let alone ones adapted to the cultural context of mental health systems. The aims of the present study were to demonstrate the feasibility and to evaluate the impact of a culturally adapted brief contact-based family-centric training program on reducing mental health-related stigma in primary healthcare workers (PHW) in a Chinese rural county. Methods: The one-day stigma reduction training program was developed based on the Opening Minds Anti-Stigma Initiative. The program included didactic lectures, case studies (two audio-taped testimonies of patient-caregiver dyads) and communication skills training (role-playing). Ten township-level and ten village-level PHW participated in separate one-day training sessions. Mental health knowledge, stigmatizing attitudes and behaviors, and provider empathy were measured before and after the training. Results: The intervention was favorably rated and considered acceptable among PHW. Compared to measures at baseline, participants’ stigmatizing attitudes reduced, their willingness to engage in relationship with people with mental illness increased, and their empathetic response toward patients improved post-intervention.Conclusions: Social contact-based family-centric training is a promising and feasible method of reducing stigma among rural Chinese PHW.
Low manganese and high iron levels in amniotic fluid correlate with fetal chromosomal abnormalities in pregnant women
Authors: Suliburska J , Pankiewicz Jakub , Sajnóg Adam , Paczkowska Magdalena , Nowakowska Beata , Bakinowska Ewa , Barałkiewicz Danuta , Kocyłowski Rafał 
Published: August 2021

Abstract
This study aimed to check the association of essential and toxic elements in amniotic fluid (AF) with chromosomal abnormalities. A total of 156 pregnant Polish white Caucasian women between the age of 20 and 43 years participated in the study. AF samples were collected during routine diagnostic and treatment procedures in pregnant women. Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the levels of various elements in AF. Genomic hybridization and cytogenic karyotype analysis were used. The results of the karyotype analysis indicated chromosomal abnormalities in 19 fetuses (over 12% of the total population) and it was mainly trisomy 21 (N=11), trisomy 18 (N=2), triploidy (N=2) and other chromosomal aberrations. It was found that a low concentration of manganese in AF was associated with chromosomal abnormalities in the foetus. High levels of iron and advanced age of the mother increased the risk of aneuploidy in the fetus. Principal component analysis (PCA) and Spearman correlation showed a strong correlation between essential and toxic elements in AF, especially in groups with chromosomal abnormalities. The results of this exploratory study indicate that the levels of essential and toxic elements in AF are associated with chromosomal abnormalities in the human fetus.
A New Remote Sensing Index for Assessing the Spatial Heterogeneity in Urban Eco-Environmental Quality Associated Road Network
Authors: Zheng Xincheng , Zou Zeyao , Xu Chongmin , Lin Sen , Wu Zhilong , Qiu Rongzu , Hu Xisheng , Li Jian 
Published: August 2021

Abstract
Although many prior efforts have found that road network has significantly affect the landscape fragmentation, the spatially heterogeneous effects of road network on the urban eco-environment remain poorly understood. To understanding the coupling mechanism of road network and eco-environment, a new remote sensing based ecological index (RSEI) was proposed to calculate the eco-environment quality, and kernel density of road (KDR) was applied to measure the road network density. We proposed a spatially explicit approach (geographically weighted regression, GWR) to explore the spatial variations in the relationship between the road network and the eco-environmental quality The results of GWR models fit better than those of the Ordinary Least Square (OLS) models. Among, the average effect of KDR on the variables of normalized difference vegetation index, land surface moisture and RSEI was negative, while it was positively associated with the variables of soil index, normalized differential build-up and bare soil index, index-based built-up index, and land surface temperature. From spatial perspective, the impacts of KDR on the urban boundary were generally greater than those on the city center. The spatial variation in the relationship between the road network and the eco-environment is mainly controlled by the relationship between the road network to the vegetation and the bare soil. Locally, the water body, whether a large regional river or urban inland rivers, has a great influence on the spatial variation in the relationship between the roads and the eco-environmental variables, and it can even change the sign of the influence.
Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties
Authors: Klanovicz Natalia , Stefanski Fábio Spitza , Camargo Aline Frumi , Michelon William , Treichel Helen , Teixeira Antonio Carlos Silva Costa 
Published: August 2021

Abstract
The occurrence of micropollutants in aqueous matrices has become a global concern and a challenge for wastewater treatment plants. Monitoring their toxicity has shown that these compounds, even at low concentrations, pose risks to human and environmental health. Therefore, our study aimed to prospect immobilization strategies for non-commercial oxidoreductase enzymes and insert them in the context of pollutant remediation. The enzymatic extract was obtained by submerged fermentation of the fungus Trichoderma koningiopsis in an alternative substrate, consisting of fresh microalgal biomass from the phycoremediation process. The immobilization efficiency of peroxidase (POD) was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the catalytic properties of free POD were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. Guaiacol peroxidase from T. koningiopsis expressed specific activity of up to 7801.1 U mg− 1 in the free form, showing stability when subjected to up 80°C in a pH range between 4.0–8.0. Furthermore, the bioproduct immobilized on magnetic nanoparticles expressed up to 688% RA and 100% DP. An increase in the RA of the enzyme, both in free and immobilized form, was also observe after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13546.4 U mg− 1 after ten cycles and removing 93% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic process of color removal with immobilized POD, despite maintaining unwanted cytotoxic effects, was essential to eliminate genotoxic effects. In this sense, the immobilization processes of T. koningiopsis peroxidase presented in our work are promising for the enzyme market and for the wastewater treatment sector.
Comparison of the Jcerity Endoscoper Airway with the LMA Supreme for Airway Management in Patients Undergoing Cerebral Aneurysm Embolization
Authors: Zhou Junfei , Li Lu , Wang Fang , Lv Yunqi 
Published: August 2021

Abstract
Background

Interventional embolization of cerebral aneurysms often requires anticoagulation and antiplatelet therapy during perioperative period. A new type of laryngeal mask airway (Jcerity Endoscoper Airway)with a unique design may cause less oropharyngeal injury and bleeding for patients receiving perioperative anticoagulation. This study sought to compare the efficacy, safety and complications of Jcerity Endoscoper airwayvs LMA((Laryngeal Mask Airway) Supreme in the procedure of cerebral aneurysm embolization.
Methods

In this prospective, randomised clinical trial, 182 adult patients with American Society of Anesthesiologists class Ι-II scheduled for interventional embolization of cerebral aneurysms were randomly allocated into the Jcerity Endoscoper airway group and the LMA Supreme group. We compared success rate of LMA implantation, ventilation quality, airway sealing pressure, peak airway pressure, degree of blood staining, postoperative oral hemorrhage, sore throat and other complications between the groups.
Results

There were no significant differences between the groups in terms of success rate of LMA implantation, ventilation quality, airway sealing pressure or airway peak pressure. The LMA Supreme group showed a significantly higher degree of blood staining than the Jcerity Endoscoper airway group when the laryngeal mask airway was removed (P = 0.04), and there were also more oral hemorrhages and pharyngeal pain than in the the Jcerity Endoscoper airway group (P = 0.03,P = 0.02). No differences were observed between groups in terms of other complications related to the LMA.
Conclusions

The Jcerity Endoscoper airway can be safely and effectively used for airway management in patients undergoing cerebral aneurysm embolization, which can significantly reduce airway complications related to perioperative anticoagulation.
Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.
Authors: Wang Huaxiang , Xu Fengfeng Feng , Lv Lizhi , Wang Ruling , Jiang Bin , Liu Tingting , Hu Huanzhang , Jiang Yi , Huang Xinghua 
Published: August 2021

Abstract
Background

Chaperonin containing TCP1 subunit 7 (CCT7), a member of the chaperonin containing TCP1 complex (CCT), has been reported regulating the expression of many tumor-related proteins. In this study, we investigated the diagnostic and prognostic value of CCT7 expression for hepatocellular carcinoma (HCC).
Methods

We investigated the CCT7 expression in HCC in The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and our cohort. The diagnostic and prognostic value were verified by receiver operating characteristic curve (ROC) analysis and Kaplan-Meier analysis, respectively. The association between CCT7 expression with DNA methylation status was investigated in the TCGA database. Gene ontology (GO), The Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) analysis were employed to identify the potential pathway in which CCT7 is involved in tumorigenesis and progression.
Results

CCT7 expression in HCC was significantly higher than adjacent normal tissues, and elevated CCT7 expression correlated with tumor stages and tumor grade. Furthermore, the ROC curve showed CCT mRNA expression has a better diagnostic value for HCC with early-stage and low alpha-fetoprotein expression. Positive predictive value (PPV) of CCT7 was higher than alpha-fetoprotein both in the GEO and TCGA database. The Multivariate Cox Regression analysis of clinicopathologic characteristics revealed that both high mRNA and protein expression of CCT7 were independent risk factors for overall survival (OS) and recurrence-free survival (RFS). High DNA methylation of CpG site(cg19515186) was associated with low CCT7 expression and better OS in HCC. The GO, KEGG and GSEA analysis demonstrated that CCT7 mRNA expression was associated with Spliceosome signaling pathway.
Conclusions

The findings of this study demonstrated that CCT7 has diagnostic and prognostic value for HCC.
Halorussus halobius sp. nov., Halorussus marinus sp. nov. and Halorussus pelagicus sp. nov., isolated from salted brown alga Laminaria
Authors: Han Dong , Cui Heng-Lin 
Published: August 2021

Abstract
Four halophilic archaeal strains, designated HD8-83T, LYG-36T, DLLS-82 and RC-68T, were isolated from the salted brown alga Laminaria of three different origins (Dalian, Lianyungang, Dalian and Rongcheng) in China. All strains had pleomorphic rod cells that were motile, lysed in distilled water, stained Gram-negative and formed red-pigmented colonies on agar plate, except DLLS-82 showing white colonies. Based on phylogenetic analyses of the 16S rRNA genes, strain HD8-83T was closely related to Halorussus litoreus HD8-51T (97.9 % similarity), strain LYG-36T and DLLS-82 were closely related to Halorussus rarus TBN4T (94.4 % and 94.7 % similarities, respectively), and strain RC-68T exhibit close to Halorussus salinus YJ-37-HT (96.9 % similarity). Phylogenetic analyses based on rpoB′ gene and 728 concatenated single-copy orthologous clusters also show these strains formed three different branches and clustered tightly with the Halorussus members. The ANI, AAI and isDDH values between strain LYG-36T and DLLS-82 were 98.9 %, 98 % and 92.4 %, showing they were different strains of same species. While those values between isolates and other Halorussus members with values below 84.7 %, 82.9 % and 28.9 %. Based on the phenotypic, chemotaxonomic and phylogenetic properties, the strains HD8-83T, LYG-36T, DLLS-82 and RC-68T represent three novel species of the genus Halorussus for which the names Halorussus halobius sp. nov. (type strain HD8-83T = CGMCC 1.15334T = JCM 31110T), Halorussus marinus sp. nov. (type strain LYG-36T = CGMCC 1.13606T = JCM 32952T, reference strain DLLS-82 = CGMCC 1.13604 = JCM 32951) and Halorussus pelagicus sp. nov. (type strain RC-68T = CGMCC 1.13609T = JCM 32953T) are proposed.
Synergistic Growth Inhibitory Effects of Eryngium kotschyi Extracts with
Conventional Cytotoxic Agents: Cisplatin and Doxorubicin against
Human Endometrium Cancer Cells
Authors: Karaboğa Arslan Ayşe Kübra , Paşayeva Leyla , Esen Merve Ayşe , Tugay Osman 
Published: August 2021

Background:
Endometrial cancer is one of the most common types of cancer. For this reason,
various studies have been carried out on its treatment and the effects of natural products on this
disease.

Objective:
This study aimed to examine the growth inhibitory effects of Eryngium kotschyi Boiss. ethyl
acetate [EKE] and butanol [EKB] obtained from the main methanol [EKM] extract from the aerial parts
on human endometrium carcinoma [RL95-2] cells and their synergistic effect with cisplatin or doxorubicin.

Methods:
RL95-2 cells were treated with E. kotschyi extracts either alone or in combination with cisplatin
or doxorubicin. The effects on cell growth were determined using the MTT assay and real-time cell
analysis xCELLigence.

Results:
The extracts demonstrated growth inhibitory activity, with a certain degree of selectivity
against the RL95-2 cell line. Synergistic effects of EKE/cisplatin or doxorubicin at different concentration
levels were demonstrated in RL95-2 cells. In some instances, the EKE/doxorubicin combinations
resulted in antagonistic effects. The reduction level of cell viability was different and specific to each
combination for the RL95-2 cell line.

Conclusion:
The growth inhibitory activity of cisplatin or doxorubicin, as a single agent, may be modified
by combinations of the extracts and be synergistically enhanced in some cases. A significant synergistic
effect of EKE on the RL95-2 cell line with cisplatin and doxorubicin was observed. This cytotoxic
effect can be investigated in terms of molecular mechanisms. This study is the first of its kind in the
literature. The mechanisms involved in this interaction between chemotherapeutic drugs and plant extracts
remain unclear and should be further evaluated.
Cytotoxic and Anti-proliferative Effects of Moringa oleifera Lam. on HeLa Cells
Authors: Govender Krishnambal , Moodley Indres , Parboosing Raveen 
Published: August 2021

Background:The aim of the study was to determine the mechanism of Moringa oleifera-induced apoptosis in HeLa cells. HeLa cells over-express cyclin E and cyclin B1, abrogate G0-G1 and G2-M cell cycle arrest, promoting tumorigenesis. Cyclin E, cyclin B1, E2F1 and telomerase expression, and caspase-3 and -7 activation were assessed after 24-treatment with M. oleifera leaf fractions.Material and methods:Apoptosis through caspase-3 and caspase-7 activation was determined quantitatively by the FAM FLICA™ Caspase-3/7 assay. Cyclin E, cyclin B1 and E2F1 were quantified by flow cytometry. Telomerase was evaluated by Telomeric repeat amplification protocol (TRAP reaction). The effects on colony formation were assessed by seeding treated cells in six-well plates for 7 days under culture conditions. The MTT assay was used to determine cell survival.Results:HeLa cells treated for 24 hours with M. oleifera leaf fractions showed dose-dependent cytotoxicity, activation of caspases-3 and -7, down regulation of cyclin E, cyclin B1, E2F1, and inhibition of telomerase expression. Cell cycle analysis of the dead cell population showed G2-M cellcycle arrest.Conclusion:M. oleifera leaf fractions triggered apoptosis through the mitochondrial pathway and cell cycle arrest at G2-M phase in HeLa cells after 24-hour treatment, through down regulation of cyclin E and cyclin B1 expression, and caspase-3 and -7 activation. In addition, M. oleifera leaf extract induces senescence in HeLa cells through the down-regulation of telomerase. Colony formation and cell proliferation were inhibited in a dose-dependent manner, corresponding with telomerase inhibition.
Variations in Patients’ Overall Assessment of Their Health Across and Within Disease Groups Using the EQ-5D Questionnaire: Protocol for a Longitudinal Study in the Swedish National Quality Registers
Authors: Teni Fitsum Sebsibe , Rolfson Ola , Devlin Nancy , Parkin David , Nauclér Emma , Burström Kristina ,   The Swedish Quality Register (SWEQR) Study Group
Published: August 2021

Background
EQ-5D is one of the most commonly used questionnaires to measure health-related quality of life. It is included in many of the Swedish National Quality Registers (NQRs). EQ-5D health states are usually summarized using “values” obtained from members of the general public, a majority of whom are healthy. However, an alternative, which remains to be studied in detail, is the potential to use patients’ self-reported overall health on the visual analog scale (VAS) as a means of capturing experience-based perspective.

Objective
The aim of this study is to assess EQ VAS as a valuation method with an experience-based perspective through comparison of its performance across and within patient groups, and with that of the general population in Sweden.

Methods
Data on nearly 700,000 patients from 12 NQRs covering a variety of diseases/conditions and nearly 50,000 individuals from the general population will be analyzed. The EQ-5D-3L data from the 12 registers and EQ-5D-5L data from 2 registers will be used in the analyses. Longitudinal studies of patient-reported outcomes among different patient groups will be conducted in the period from baseline to 1-year follow-up. Descriptive statistics and analyses comparing EQ-5D dimensions and observed self-assessed EQ VAS values across and within patient groups will be performed. Comparisons of the change in health state and observed EQ VAS values at 1-year follow-up will also be undertaken. Regression models will be used to assess whether EQ-5D dimensions predict observed EQ VAS values to investigate patient value sets in each patient group. These will be compared across the patient groups and with the existing Swedish experience-based VAS and time trade-off value sets obtained from the general population.

Results
Data retrieval started in May 2019 and data of patients in the 12 NQRs and from the survey conducted among the general population have been retrieved. Data analysis is ongoing on the retrieved data.

Conclusions
This research project will provide information on the differences across and within patient groups in terms of self-reported health status through EQ VAS and comparison with the general population. The findings of the study will contribute to the literature by exploring the potential of self-assessed EQ VAS values to develop value sets using an experience-based perspective.

Trial Registration
ClinicalTrials.gov NCT04359628; https://clinicaltrials.gov/ct2/show/NCT04359628.

International Registered Report Identifier (IRRID)
DERR1-10.2196/27669
EVIDÊNCIAS DA PLASTICIDADE E ATUALIDADE DA DIVISÃO SEXUAL DO TRABALHO A PARTIR DE QUATRO MODALIDADES CONCRETAS
Authors: Briguglio Bianca , Grecco Fabiana Sanches , Lindôso Raquel Oliveira , Lapa Thaís de Souza 
Published: August 2021

Neste artigo, partimos de quatro diferentes modalidades concretas de trabalho: na indústria metalúrgica; na indústria de confecções; nas cozinhas de restaurantes; e no trabalho de catação de materiais recicláveis, para evidenciar a divisão sexual do trabalho, conforme as sociólogas do trabalho, feministas materialistas, Helena Hirata e Danièle Kergoat, a compreendem: como expressão da base material das relações de gênero na sociedade capitalista, regida pelos princípios de separação e de hierarquia, que organizam a divisão social do trabalho entre os gêneros e distribui desigualmente os trabalhos produtivos, domésticos e de cuidados. Com isso, reafirmamos essa concepção de divisão sexual do trabalho como uma chave de leitura teórico metodológica atual, com caráter plástico e comparativo.
ceito.
Das naturezas que se movem pelo afeto na poesia de Sónia Sultuane
Authors: Freitas Sávio 
Published: August 2021

Sónia Sultuane é uma escritora e artista plástica que vem ocupando lugar de destaque na cena cultural de Moçambique. A coletânea de poemas No colo da Lua (2009) revela uma voz poética no feminino que se relaciona com natureza na perspectiva sinalizada pelos estudos ecofeministas referente ao endosso de um exercício humanístico de proteger o mundo e as mais diversas manifestações da natureza. Para fundamentar esta localização epistemológica vamos ancorar nos posicionamentos de Angélica Soares (1999) sobre erotismo e ecologia, de Carmen Tindó Secco (2014) sobre as reflexões em torno de afeto e poesia, de Greta Gaard (2017) sobre o rumos do ecofeminismo, e de Izabel Brandão (2003) sobre as fronteiras críticas do ecofeminismo e da literatura.
Palavras-Chave: Ecofeminismo. Poesia,. Afeto. Sónia Sultuane.
Dialogando com as línguas selvagens: contribuições de Gloria Anzaldúa para pensar o feminismo decolonial
Authors: Da Veiga Silva Vivian 
Published: August 2021

A decolonialidade é uma proposta epistemológica construída a partir da obra de Aníbal Quijano (1930-2018) que busca romper com a colonialidade imposta às sociedades colonizadas, de maneira a criar e manter hierarquizações e classificações subalternizantes. Diversas teóricas feministas levaram essa perspectiva teórica para o campo feminista, forjando o que podemos denominar de feminismo decolonial. Sua proposta central é construir uma agenda feminista focada nas vozes e nas experiências das mulheres situadas ao Sul, nas sociedades que sofreram intrusões coloniais. Nessa perspectiva, a proposta do presente artigo é estabelecer um diálogo entre o feminismo decolonial e a teórica chicana Gloria Anzaldúa (1942-2004), considerada um dos expoentes do feminismo descolonizado.
Palavras-chave: Gloria Anzaldúa. Decolonialidade. Feminismo decolonial. Norte. Sul