bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of an epithelial-mesenchymal transition-related lncRNA prognostic signature for patients with glioblastoma
Authors: Yang XinJie , Niu Sha , Liu JiaQiang , Fang Jincheng , Wu ZeYu , Ling Shizhang , Di GuangFu , Jiang XiaoChun 
Published: December 2021

AbstractGlioblastoma (GBM) is a strikingly heterogeneous and lethal brain tumor with very poor prognosis. LncRNAs play critical roles in the tumorigenesis of GBM through regulation of various cancer-related genes and signaling pathways. Here, we focused on the essential role of EMT and identified 78 upregulated EMT-related genes in GBM through differential expression analysis and Gene set enrichment analysis (GSEA). A total of 301 EMT-related lncRNAs were confirmed in GBM through Spearman correlation analysis and a prognostic signature consisting of seven EMT-related lncRNAs (AC012615.1, H19, LINC00609, LINC00634, POM121L9P, SNHG11, and USP32P3) was established by univariate and multivariate Cox regression analyses. Significantly, Kaplan–Meier analysis and receiver-operating-characteristic (ROC) curve validated the accuracy and efficiency of the signature to be satisfactory. Quantitative real-time (qRT)-PCR assay demonstrated the expression alterations of the seven lncRNAs between normal glial and glioma cell lines. Functional enrichment analysis revealed multiple EMT and metastasis-related pathways were associated with the EMT-related lncRNA prognostic signature. In addition, we observed the degree of immune cell infiltration and immune responses were significantly increased in high-risk subgroup compared with low-risk subgroup. In conclusion, we established an effective and robust EMT-related lncRNA signature which was expected to predict the prognosis and immunotherapy response for GBM patients.
Elevated resting heart rate is associated with increased radiographic severity of knee but not hand joints
Authors: Choi Sung-Eun , Xu Haimuzi , Kang Ji-Hyoun , Park Dong-Jin , Kweon Sun-Seog , Lee Young-Hoon , Kim Hye-Yeon , Lee Jung-Kil , Shin Min-Ho , Lee Shin-Seok 
Published: December 2021

AbstractAlthough the resting heart rate (RHR) predicts the clinical outcomes of cardiovascular disease, chronic obstructive lung disease, diabetes mellitus, and the risk of cancer, its role in patients with musculoskeletal diseases, such as osteoarthritis (OA), remains unclear. We explored the association of the RHR with the extents of radiographic changes in the knees and hands of 2369 subjects from the Dong-gu Study. The radiographic hand and knee joint findings were graded semi-quantitatively; we calculated total hand and knee joint scores. Multiple linear regression was performed to examine the associations between the RHR and the radiographic characteristics of these joints. For the knee joints, the RHR was associated positively with the total (p < 0.01), osteophyte (p < 0.01), joint space narrowing (JSN; p < 0.01), and tibial attrition (p = 0.02) scores after adjustment for age, sex, body mass index, smoking status, alcohol consumption, educational and physical activity levels, and comorbidities. For the hand joints, the RHR was associated positively with the JSN (p = 0.01) and subchondral cyst (p < 0.01) scores after such adjustment. The RHR was not associated with the total, osteophyte, sclerosis, erosion, or malalignment score for the hand joints. This study is the first to reveal an association between the RHR and the radiographic severity of knee, but not hand, OA.
Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12
Authors: Sandhu Sumit , Sou Ieng F. , Hunter Jill E. , Salmon Lucy , Wilson Caroline L. , Perkins Neil D. , Hunter Neil , Davies Owen R. , McClurg Urszula L. 
Published: December 2021

AbstractThe synaptonemal complex (SC) is a supramolecular protein scaffold that mediates chromosome synapsis and facilitates crossing over during meiosis. In mammals, SC proteins are generally assumed to have no other function. Here, we show that SC protein TEX12 also localises to centrosomes during meiosis independently of chromosome synapsis. In somatic cells, ectopically expressed TEX12 similarly localises to centrosomes, where it is associated with centrosome amplification, a pathology correlated with cancer development. Indeed, TEX12 is identified as a cancer-testis antigen and proliferation of some cancer cells is TEX12-dependent. Moreover, somatic expression of TEX12 is aberrantly activated via retinoic acid signalling, which is commonly disregulated in cancer. Structure-function analysis reveals that phosphorylation of TEX12 on tyrosine 48 is important for centrosome amplification but not for recruitment of TEX12 to centrosomes. We conclude that TEX12 normally localises to meiotic centrosomes, but its misexpression in somatic cells can contribute to pathological amplification and dysfunction of centrosomes in cancers.
Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1
Authors: Harding Rachel J. , Deme Justin C. , Hevler Johannes F. , Tamara Sem , Lemak Alexander , Cantle Jeffrey P. , Szewczyk Magdalena M. , Begeja Nola , Goss Siobhan , Zuo Xiaobing , Loppnau Peter , Seitova Alma , Hutchinson Ashley , Fan Lixin , Truant Ray , Schapira Matthieu , Carroll Jeffrey B. , Heck Albert J. R. , Lea Susan M. , Arrowsmith Cheryl H. 
Published: December 2021

AbstractHuntington’s disease results from expansion of a glutamine-coding CAG tract in the huntingtin (HTT) gene, producing an aberrantly functioning form of HTT. Both wildtype and disease-state HTT form a hetero-dimer with HAP40 of unknown functional relevance. We demonstrate in vivo and in cell models that HTT and HAP40 cellular abundance are coupled. Integrating data from a 2.6 Å cryo-electron microscopy structure, cross-linking mass spectrometry, small-angle X-ray scattering, and modeling, we provide a near-atomic-level view of HTT, its molecular interaction surfaces and compacted domain architecture, orchestrated by HAP40. Native mass spectrometry reveals a remarkably stable hetero-dimer, potentially explaining the cellular inter-dependence of HTT and HAP40. The exon 1 region of HTT is dynamic but shows greater conformational variety in the polyglutamine expanded mutant than wildtype exon 1. Our data provide a foundation for future functional and drug discovery studies targeting Huntington’s disease and illuminate the structural consequences of HTT polyglutamine expansion.
Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility
Authors: Olivieri Anna , Lee Rebecca S. , Fratini Federica , Keutcha Cyrianne , Chaand Mudit , Mangano Valentina , Celani Francesco , Mochi Stefania , Birago Cecilia , Paone Silvio , Grasso Felicia , Tirelli Valentina , Falchi Mario , Shabani Estela , Bertoncini Stefania , Sirima Bienvenu Sodiomon , Pizzi Elisabetta , Modiano David , Duraisingh Manoj T. , Ponzi Marta 
Published: December 2021

AbstractCholesterol-rich microdomains are membrane compartments characterized by specific lipid and protein composition. These dynamic assemblies are involved in several biological processes, including infection by intracellular pathogens. This work provides a comprehensive analysis of the composition of human erythrocyte membrane microdomains. Based on their floating properties, we also categorized the microdomain-associated proteins into clusters. Interestingly, erythrocyte microdomains include the vast majority of the proteins known to be involved in invasion by the malaria parasite Plasmodium falciparum. We show here that the Ecto-ADP-ribosyltransferase 4 (ART4) and Aquaporin 1 (AQP1), found within one specific cluster, containing the essential host determinant CD55, are recruited to the site of parasite entry and then internalized to the newly formed parasitophorous vacuole membrane. By generating null erythroid cell lines, we showed that one of these proteins, ART4, plays a role in P. falciparum invasion. We also found that genetic variants in both ART4 and AQP1 are associated with susceptibility to the disease in a malaria-endemic population.
High stability and metabolic capacity of bacterial community promote the rapid reduction of easily decomposing carbon in soil
Authors: Huang Ruilin , Crowther Thomas W. , Sui Yueyu , Sun Bo , Liang Yuting 
Published: December 2021

AbstractIrreversible climate change alters the decomposition and sequestration of soil carbon (C). However, the stability of C components in soils with different initial organic matter contents and its relationship with the response of major decomposers to climate warming are still unclear. In this study, we translocated Mollisols with a gradient of organic matter (OM) contents (2%–9%) from in situ cold region to five warmer climatic regions to simulate climate change. Soil C in C-rich soils (OM >5%) was more vulnerable to translocation warming than that in C-poor soils (OM ≤ 5%), with a major loss of functional groups like O-alkyl, O-aryl C and carboxyl C. Variations of microbial β diversity with latitude, temperature and precipitation indicated that C-rich soils contained more resistant bacterial communities and more sensitive fungal communities than C-poor soils, which led to strong C metabolism and high utilization ability of the community in C-rich soils in response to translocation warming. Our results suggest that the higher sensitivity of soils with high organic matter content to climate change is related to the stability and metabolic capacity of major bacterial decomposers, which is important for predicting soil-climate feedback.
Multi‐label based view learning for vehicle re‐identification
Authors: Liu Yichu , Hu Haifeng , Chen Dihu 
Published: December 2021

AbstractDue to the high similarity of different vehicles with similar appearances and the great diversity of camera viewpoints, vehicle re‐identification (ReID) is still a challenging task. It commonly maps query set image into a high dimensional embedding space and then retrieve gallery vehicle images according to the distance. In this letter, a multi‐label based view learning (MLVL) model to enhance the distinguishability of intra‐class and inter‐class through multiple labels learning, and decrease the distance of intra‐class features caused by multi‐view appearances of vehicles. Specifically, the model includes two main parts. First, the vehicle orientation estimation module is responsible for detecting key‐points of the vehicle and predicting its orientation, which provides the orientation label for the multi‐label based learning network to enhance view‐invariant representation. Second, a novel dual‐triplet and quadruplet loss function is designed to optimise intra‐class and inter‐class distance with the help of multi‐label information, i.e. vehicle colour, type and orientation. In particular, a multi‐label based sampler is proposed to generate training mini‐batches instead of random sampler. Extensive experiments results show that the proposed MLVL model achieves 5.6 mAP improvement on VeRi‐776 and 1.9 on VERI‐Wild datasets compared with baseline model.
Improved design and analysis of a radial magnetic bearing with paired‐pole or alternating‐pole configurations
Authors: Xu Shilei , Sun Jinji , Wang Zhipeng 
Published: December 2021

AbstractThe magnetic saturation of iron‐core will reduce the maximum output force, influence the linear force characteristic and control precision of the magnetic bearing. To avoid these problems, a modified iron‐core design rule for the alternating‐pole magnetic bearing is proposed. The thickness of both stator yoke and rotor journal should be designed as 3/4 of the stator pole width for the alternating‐pole configuration, which is successfully verified by 3D finite element method and experiments. Based on the improved design, the structural sizes, rotor iron losses, and force coupling characteristics of the paired‐pole configuration and the alternating‐pole configuration are compared. The results show that the size of the alternating‐pole bearing is smaller than that of the paired‐pole bearing; the alternating‐pole configuration generates slightly higher rotor iron loss than the paired‐pole configuration; the coupling strength of the alternating‐pole configuration is stronger than that of the paired‐pole configuration irrespective of whether the magnetic force coupling is caused by rotor displacements or control currents. The presented method and results can provide a reference for the design and selection of the active magnetic bearings.
Deep‐learning‐based line enhancer for passive sonar systems
Authors: Ju Donghao , Chi Cheng , Li Zigao , Li Yu , Zhang Chunhua , Huang Haining 
Published: December 2021

AbstractDetection of acoustic tonals from surfaces and underwater vehicles is important for passive sonar systems. Enhancements of the tonals are usually necessary in passive sonar prior to detections. Conventionally, passive sonars employ adaptive line enhancers (ALE) in order to realise enhancements of the tonals. However, ALEs have requirements on their input signal‐to‐noise ratios (SNR). When the SNR inputs are too low, the ALEs cannot perform well. Therefore, for the purpose of overcoming the limitations of the SNR inputs to ALEs, this study proposes to enhance tonals using unsupervised deep‐learning techniques. The proposed deep‐learning‐based line enhancer (DLE) is based on an autoencoder neural network. The simulation results show that when the input SNR is −28 dB, the proposed DLE still achieves an SNR gain of 15 dB. However, the reference ALE fails. The experimental results also demonstrate the superiority of the proposed DLE.
Herzratenvariabilitätsbasiertes Lifestyle-Coaching bei Patient*innen mit subakromialem Schmerzsyndrom – eine Pilotstudie
Authors: Kalmring Matthias 
Published: December 2021

Zusammenfassung
Hintergrund Mehrere Studien konnten einen Einfluss von psychologischem Stress auf Schmerzmodulation und Wundheilung aufzeigen. Die Erweiterung der physiotherapeutischen Behandlung um die psychosoziale Ebene des biopsychosozialen Modells stellt komplexe Anforderungen an die behandelnden Physioherapeut*innen.
Ziel Untersucht wurde der Einfluss von auf Herzratenvariabilität (HRV) basierendem Lifestyle-Coaching auf die Entwicklung der funktionellen Einschränkungen und das Schmerzempfinden bei Patient*innen mit subakromialem Schmerzsyndrom (SAPS). Es erfolgte zudem eine Analyse der Machbarkeit für Folgestudien.
Methode 15 Proband*innen mit SAPS wurden randomisiert in 2 Gruppen eingeteilt. Die Interventionsgruppe erhielt zusätzlich zu der in beiden Gruppen durchgeführten übungstherapeutischen Intervention ein Lifestyle-Coaching. Als Kontrollparameter wurden der SPADI-Score (SPADI), das maximale Schmerzempfinden (NRSmax) und anhand der Kurzzeit-HRV-Messung die High Frequency (HF), Low Frequency (LF), LF/HF-Ratio, Root Mean Square of Successive Difference (Rmssd) sowie die Standard Deviation NN (SdNN) erhoben. Bezüglich der Machbarkeit wurden mögliche Störfaktoren, Optimierungsmöglichkeiten und eine adäquate Stichprobengröße eruiert.
Ergebnisse Einen signifikanten Unterschied zeigten die Variablen des SPADI-Scores, SPADI (95 % CI –59,3 bis –4,6; p = 0,026; d = –1,5), NRSmax (95 % CI –5,5 bis –0,1; p = 0,042; d = –1,35) und der HRV-Messwert HF (95 % CI 505,3–1753,3; p = 0,002; d = 2,23) im Vergleich zur Kontrollgruppe.
Schlussfolgerung Das Design dieser Studie ist machbar und für Folgestudien mit größeren Stichproben geeignet. Anpassungen bei der Randomisierung sowie den verwendeten Assessments sollten dabei vorgenommen werden. Für eine gültige Aussagekraft der Ergebnisse wurde die dafür nötige Stichprobengröße mit n = 66 ermittelt. Die Auswertung der klinischen Parameter weisen auf eine Steigerung der parasympathischen Aktivität (HF) sowie einer Reduktion von SPADI und NRSmax hin. Letztere können hierbei als potentiell positive Wirkung auf die Funktion und Schmerzreduktion in der Interventionsgruppe eingeschätzt werden.
Orthognathe Chirurgie und CMD – was ist erwiesen?
Authors: Kolk Andreas , Walch Benjamin 
Published: December 2021

ZusammenfassungKraniomandibuläre Dysfunktionen (CMD) und deren Stellenwert für die orthognathe Chirurgie (OGC) wurden in den letzten Jahren kontrovers diskutiert. Das durch vielfältige Symptome gekennzeichnete Bild einer CMD hat funktionelle und psychogene Einflüsse und beeinträchtigt die Lebensqualität der Betroffenen. Ätiologie und Pathophysiologie der CMD sind nur teilweise verstanden; die Fluktuation der CMD-Symptome mit On- und Off-Phasen erschwert die Analyse unter Studienbedingungen. Ob OGC eine CMD verbessert oder gar verschlechtert ist die Kardinalfrage, die sich stellt und auf die dieser Übersichtsartikel eine Antwort geben soll. Obwohl zahlreiche Studien zu diesem Themenbereich vorliegen, ist die Beantwortung der Frage des Kausalzusammenhanges zwischen CMD und OGC nicht eindeutig möglich. Der überwiegende Teil der Studien unterstützt die Annahme, dass eine CMD durch OGC eher verbessert wird. Relativ einig ist man sich auch dabei, dass eine vor OGC bestehende CMD präoperativ beseitigt bzw. zumindest deutlich gemildert werden muss, da es sonst zu einer Symptomverstärkung kommen kann. Zusätzlich werden Risikofaktoren beleuchtet, die eine OGC-bedingte CMD begünstigen können.
Aligner-Systeme in der Kieferorthopädie: ein aktueller Überblick
Authors: Gänzer Hanna 
Published: December 2021

ZusammenfassungIm kieferorthopädischen Alltag ist es keine Seltenheit mehr, dass die Patienten eine möglichst rasche und unkomplizierte, aber v. a. ästhetische Behandlung bevorzugen. Das ist zumindest der Wunsch des Patienten.Daher wurden die Energien der letzten Jahre vermehrt auf die Entwicklung durchsichtiger Schienen zur Behandlung von Zahnfehlstellungen gelenkt, was zu einer Übersättigung des Marktes geführt hat. Bei Recherchen im Internet konnten im Jahre 2015 bereits 27 unterschiedliche Anbieter von Alignern gelistet werden.Den Ursprung fanden die Aligner jedoch schon Jahre zuvor, beginnend mit dem Gedanken, kleine Zahnfehlstellungen wie rotierte Zähne oder geringe Engstände der Frontzähne zu beheben. Alle Schienen teilen als große Gemeinsamkeit, dass sie als durchsichtige, temperaturgeformte Plastikschiene den gesamten Zahnbogen umfassen, sonst können die Modelle der verschiedenen Anbieter allerdings sehr unterschiedlich ausfallen bzw. ausgestattet sein, entsprechend den Wünschen des behandelnden Arztes.Auch heute gibt es noch Aligner-Systeme, welche sich explizit auf die Behandlung kleiner Zahnfehlstellungen und Irregularitäten spezialisiert haben, mit anderen Aligner-Systemen ist es jedoch möglich auch komplexe Zahnfehlstellungen zu behandeln.Gerade um größere Zahnbewegungen durchführen zu können, werden von einigen Aligner-Systemen Attachments für die Behandlung geplant. Durch diese zahnfarbenen Knöpfchen, welche an der bukkalen Fläche des Zahnes angebracht werden, können Kräfte appliziert werden, die sonst nicht möglich wären.Studien, welche die Wirkungsweise von Alignern allein oder den Vergleich mit fixen Apparaturen aufzeigen, wurden in der Literatur aufgrund fehlender Kontrollgruppen, fehlender Verblindung bzw. Randomisierung oder schlechter Methodik oft als mangelhaft beschrieben 1.
Konservative stationäre Behandlung von spezifischen
Rückenschmerzen vor und während der Corona-Pandemie
Authors: Haase Ingo , Kladny Bernd 
Published: December 2021

Zusammenfassung
Hintergrund Als Fach mit einem sehr hohen Anteil elektiv planbarer
Operationen war die Orthopädie und Unfallchirurgie strukturell und
finanziell stark von den Maßnahmen gegen die Coronapandemie betroffen.
Erfahrungen aus der konservativen Orthopädie wurden bisher kaum
berichtet.
Ziel Die Studie soll Erkenntnisse darüber liefern, welche
Veränderungen sich im Patientenkollektiv einer stationären konservativen
Behandlung von Patienten mit Wirbelsäulenerkrankungen unter der
Coronapandemie hinsichtlich Fallzahlen, Patientenstruktur und
Behandlungsverlauf ergeben haben.
Material und Methoden Es wurden retrospektiv Daten von insgesamt 954
Patienten aus der Routinedokumentation einer Abteilung für konservative
Behandlung von Rückenschmerzen aus den Jahren 2019 und 2020 verglichen, die
Aussagen über den Verlauf der stationären konservativen
Wirbelsäulenbehandlung erlauben. Neben soziodemografischen Daten wurden
numerische Schmerzratingskalen, Skalen zur Beeinträchtigung durch die
Schmerzen und zur körperlichen Funktionsfähigkeit bei Behandlungsbeginn und
bei Behandlungsende mittels deskriptiver Statistik und Unterschiedstests
analysiert.
Ergebnisse Die Studie zeigte eine Reduktion der Fallzahlen um 21%
gegenüber dem Zeitraum vor der Pandemie. Die Patientenstruktur hat sich
geändert hinsichtlich der Diagnosegruppen und der körperlichen
Funktionsfähigkeit. Die Werte der Assessments bei Entlassung wie auch deren
Prä-post-Differenzen zeigen ein nahezu identisches Bild der Ergebnisqualität
vor und nach Pandemiebeginn.
Diskussion Der relativ geringe Rückgang an stationären Einweisungen
zur nicht operativen Behandlung von spezifischen Wirbelsäulenerkrankungen
verweist darauf, dass auch in der pandemiebedingten Krise dieses
Behandlungsangebot erforderlich war. Bei geringen Veränderungen in der
Patientenzusammensetzung konnten vergleichbare Behandlungsergebnisse erzielt
werden.
Changes in Pulmonary Function After Long-duration Adventure Racing in
Adolescent Athletes
Authors: Birat Anthony , Garnier Yoann , Dodu Alexandre , Grossoeuvre Claire , Dupont Anne-Charlotte , Mucci Patrick , Blazevich Anthony , Rance Mélanie , Morel Claire , Nottin Stéphane , Ratel Sebastien 
Published: December 2021

AbstractThe present study investigated the acute effects of a mixed-modality,
long-duration adventure race on pulmonary function in adolescent athletes.
Twenty male adolescents aged 14 to 17 years volunteered to participate in a
wilderness adventure race of 68.5-km. Expiratory function was evaluated before,
immediately after, and 24 h after race completion. Measurements included
forced vital capacity (FVC), forced expiratory volume in 1 s
(FEV1) and peak expiratory flow (PEF). Maximal inspiratory and
expiratory mouth static pressures (MIP and MEP, respectively) were also measured
using a portable hand-held mouth pressure meter across the same time points. The
mean completion time of the race was 05:38±00:20 hours. A
significant post-race decrease in FVC was observed immediately after the race
(−5.2%, p=0.01). However, no significant changes were
observed for FEV1, PEF and the FEV1/FVC and
FEV1/PEF ratios. In addition, estimates of respiratory
muscle strength (MIP and MEP) were unaffected by the race. The long-duration
adventure race induced no marked reduction in expiratory pulmonary function and
this response was associated with no apparent respiratory muscle fatigue.
Therefore, the pulmonary system of trained adolescent athletes was sufficiently
robust to sustain the mixed-modality, long-duration adventure race of
~ 5–6 h.
Giant Distal Anastomotic Pseudoaneurysm 35 Years after Bentall Operation Mimicking an “Elephant Trunk”
Authors: Scaffa Raffaele , Torre Mario , Longobardi Antonio , Ferrara David , Vassallo Maria G. , Itri Francesco , Coscioni Enrico 
Published: December 2021

AbstractWe present the case of a giant distal aortic pseudoaneurysm 35 years after a classic mechanical Bentall operation. Computed tomography and coronary angiography showed that this originated from the distal suture line. The proximal suture and coronary ostia appeared to be intact. At reoperation, we found a complete dehiscence of distal suture line: the graft was floating in the pseudoaneurysm, mimicking an “elephant trunk” procedure. This complication suggested a systematic and accurate follow-up of patients who underwent an original Bentall procedure.