bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and determinants of serum antibodies to SARS-CoV-2 in the general population of the Gardena valley
Authors: Melotti Roberto , Scaggiante Federica , Falciani Michela , Weichenberger Christian X. , Foco Luisa , Lombardo Stefano , De Grandi Alessandro , von Laer Dorothee , Mahlknecht Angelika , Pramstaller Peter P. , Pagani Elisabetta , Meier Horand , Gaertner Timon , Troi Christina , Mascalzoni Deborah , Pattaro Cristian , Mian Michael 
Published: August 2021

Abstract
Estimating the spread of SARS-CoV-2 infection in communities is critical. We surveyed 2244 stratified random sample community members of the Gardena valley, a winter touristic area, amidst the first expansion phase of the COVID-19 pandemic in Europe. We measured agreement between Diasorin and Abbott serum bioassay outputs and the Abbott optimal discriminant threshold of serum neutralisation titres with recursive receiver operating characteristic curve. We analytically adjusted serum antibody tests for unbiased seroprevalence estimate and analysed the determinants of infection with non-response weighted multiple logistic regression. SARS-CoV-2 seroprevalence was 26.9% (95% CI 25.2–28.6) by June 2020. The bioassays had a modest agreement with each other. At a lower threshold than the manufacturer's recommended level, the Abbott assay reflected greater discrimination of serum neutralisation capacity. Seropositivity was associated with place and economic activity, not with sex or age. Symptoms like fever and weakness were age-dependent. SARS-CoV-2 mitigation strategies should account for context in high prevalence areas.
Origin, Accretion, and Reworking of Continents
Authors: Zhu Rixiang , Zhao Guochun , Xiao Wenjiao , Chen Ling , Tang Yanjie 
Published: August 2021

AbstractThe continental crust is unique to the Earth in the solar system, and controversies remain regarding its origin, accretion and reworking of continents. The plate tectonics theory has been significantly challenged in explaining the origin of Archean (especially pre‐3.0 Ga) continents as they rarely preserve hallmarks of plate tectonics. In contrast, growing evidence emerges to support oceanic plateau models that better explain characteristics of Archean continents, including the bimodal volcanics and nearly coeval emplacement of tonalite‐trondjhemite‐granodiorite (TTG) rocks, presence of ∼1600°C komatiites and dominant dome structures, and lack of ultra‐high‐pressure rocks, paired metamorphic belts and ophiolites. On the other hand, the theory of plate tectonics has been successfully applied to interpret the accretion of continents along subduction zones since the late Archean (3.0–2.5 Ga). During subduction processes, the new mafic crust is generated at the base of continents through partial melting of mantle wedge with the addition of H2O‐dominant fluids from subducted oceanic slabs and partial melting of the juvenile mafic crust results in the generation of new felsic crusts. This eventually leads to the outgrowth of continents. Subduction processes also cause softening, thinning, and recycling of continental lithosphere due to the vigorous infiltration of volatile‐rich fluids and melts, especially along weak belts/layers, leading to widespread continental reworking and even craton destruction. Reworking of continents also occurs in continental interiors due to either plate boundary processes or plume‐lithosphere interactions. The effects of plumes have proven to be less significant and cause lower degrees of lithospheric modification than subduction‐induced craton destruction.
Increasing Difference in Interannual Summertime Surface Air Temperature Between Interior East Antarctica and the Antarctic Peninsula Under Future Climate Scenarios
Authors: Mao Rui , Kim Seong‐Joong , Gong Dao‐Yi , Liu Xiaohong , Wen Xinyu , Zhang Liping , Tang Feng , Zong Qi , Xiao Cunde , Ding Minghu , Park Sang‐Jong 
Published: August 2021

Plain Language SummaryMelting of the Antarctic ice sheet and shelf in the future will be influenced by interannual changes in the surface air temperature (SAT) in Antarctica. The SAT changes in Antarctica are related to variations in the Southern Hemisphere Annular Mode (SAM) during the austral summer. The SAM is a dominant pattern of atmospheric variability in the Southern Hemisphere and influences the Antarctic SAT with opposite changes between the northern Antarctic Peninsula (AP) and Eastern Antarctica (EA). To project future changes in the Antarctic SAT, we analyzed historical and future simulations from the Climate Model Intercomparison Project 5 models. We found that the degree of opposite interannual SAT changes between EA and the AP increases in the future due to intensified magnitude of the SAM‐related circulation anomalies, and summers of warmer SAT in the northern AP and cooler SAT in EA increase by 4% in the future compared to the historical period. This finding has major consequences for glacier melting in the northern AP in the future because more days of extremely high SAT in the northern AP may occur in the future.
Gas Geochemistry at Grande Comore and Mayotte Volcanic Islands (Comoros Archipelago), Indian Ocean
Authors: Liuzzo Marco , Di Muro Andrea , Rizzo Andrea Luca , Caracausi Antonio , Grassa Fausto , Fournier Nicolas , Shafik Bafakih , Boudoire Guillaume , Coltorti Massimo , Moreira Manuel , Italiano Francesco 
Published: August 2021

AbstractThe Comoros archipelago is an active geodynamic region of intra‐plate volcanism within which the youngest and oldest islands (Grande Comore and Mayotte respectively) are characterized by recent volcanic activity. The frequent eruptions of the large shield volcano Karthala on Grande Comore (last eruption 2007), and the recent birth of a large submarine volcano since 2018 at the submarine base of Mayotte are associated with permanent fumarolic emissions, bubbling gas seeps, and soil gas emissions, which are studied in detail here for the first time. CO2 fluxes and chemical and isotopic gas compositions acquired during two surveys in 2017 and 2020 are integrated with older data sets collected between 2005 and 2016, permitting the identification of a possible influence of the recent volcanic and magmatic activity at Mayotte. At Karthala, high gas fluxes with high temperature, and a marked magmatic signature are concentrated close to the summit crater area, while only weaker emissions with a stronger biogenic signature are found on the volcano flanks. At Mayotte, lower temperature and higher CH4 content are recorded in two main seep areas of CO2‐rich fluid bubbling, while soil emissions on land record a higher proportion of magmatic fluids compared to Karthala. Our preliminary results reveal two quite separate gas emission patterns for each island that are distinct in composition and isotopic signatures, and well correlated with the present state of volcanic activity. This work may potentially provide support for local observation infrastructures and contribute to the improvement in volcanic and environmental monitoring.
miR-22 promotes stem cell traits via activating Wnt/β-catenin signaling in cutaneous squamous cell carcinoma
Authors: Yuan Shukai , Zhang Peitao , Wen Liqi , Jia Shikai , Wu Yufan , Zhang Zhenlei , Guan Lizhao , Yu Zhengquan , Zhao Li 
Published: August 2021

AbstractEmerging evidence suggests that the cancer stem cells (CSCs) are key culprits of cancer metastasis and drug resistance. Understanding mechanisms regulating the critical oncogenic pathways and CSCs function could reveal new diagnostic and therapeutic strategies. We now report that miR-22, a miRNA critical for hair follicle stem/progenitor cell differentiation, promotes tumor initiation, progression, and metastasis by maintaining Wnt/β-catenin signaling and CSCs function. Mechanistically, we find that miR-22 facilitates β-catenin stabilization through directly repressing citrullinase PAD2. Moreover, miR-22 also relieves DKK1-mediated repression of Wnt/β-catenin signaling by targeting a FosB-DKK1 transcriptional axis. miR-22 knockout mice showed attenuated Wnt/β-catenin activity and Lgr5+ CSCs penetrance, resulting in reduced occurrence, progression, and metastasis of chemically induced cutaneous squamous cell carcinoma (cSCC). Clinically, miR-22 is abundantly expressed in human cSCC. Its expression is even further elevated in the CSCs proportion, which negatively correlates with PAD2 and FosB expression. Inhibition of miR-22 markedly suppressed cSCC progression and increased chemotherapy sensitivity in vitro and in xenograft mice. Together, our results revealed a novel miR-22-WNT-CSCs regulatory mechanism in cSCC and highlight the important clinical application prospects of miR-22, a common target molecule for Wnt/β-catenin signaling and CSCs, for patient stratification and therapeutic intervention.
Transcriptional CDK inhibitors, CYC065 and THZ1 promote Bim-dependent apoptosis in primary and recurrent GBM through cell cycle arrest and Mcl-1 downregulation
Authors: Juric Viktorija , Hudson Lance , Fay Joanna , Richards Cathy E. , Jahns Hanne , Verreault Maïté , Bielle Franck , Idbaih Ahmed , Lamfers Martine L. M. , Hopkins Ann M. , Rehm Markus , Murphy Brona M. 
Published: August 2021

AbstractActivation of cyclin-dependent kinases (CDKs) contributes to the uncontrolled proliferation of tumour cells. Genomic alterations that lead to the constitutive activation or overexpression of CDKs can support tumourigenesis including glioblastoma (GBM), the most common and aggressive primary brain tumour in adults. The incurability of GBM highlights the need to discover novel and more effective treatment options. Since CDKs 2, 7 and 9 were found to be overexpressed in GBM, we tested the therapeutic efficacy of two CDK inhibitors (CKIs) (CYC065 and THZ1) in a heterogeneous panel of GBM patient-derived cell lines (PDCLs) cultured as gliomaspheres, as preclinically relevant models. CYC065 and THZ1 treatments suppressed invasion and induced viability loss in the majority of gliomaspheres, irrespective of the mutational background of the GBM cases, but spared primary cortical neurons. Viability loss arose from G2/M cell cycle arrest following treatment and subsequent induction of apoptotic cell death. Treatment efficacies and treatment durations required to induce cell death were associated with proliferation velocities, and apoptosis induction correlated with complete abolishment of Mcl-1 expression, a cell cycle-regulated antiapoptotic Bcl-2 family member. GBM models generally appeared highly dependent on Mcl-1 expression for cell survival, as demonstrated by pharmacological Mcl-1 inhibition or depletion of Mcl-1 expression. Further analyses identified CKI-induced Mcl-1 loss as a prerequisite to establish conditions at which the BH3-only protein Bim can efficiently induce apoptosis, with cellular Bim amounts strongly correlating with treatment efficacy. CKIs reduced proliferation and promoted apoptosis also in chick embryo xenograft models of primary and recurrent GBM. Collectively, these studies highlight the potential of these novel CKIs to suppress growth and induce cell death of patient-derived GBM cultures in vitro and in vivo, warranting further clinical investigation.
Thromboembolism after COVID-19 vaccine in patients with preexisting thrombocytopenia
Authors: Mauriello Alessandro , Scimeca Manuel , Amelio Ivano , Massoud Renato , Novelli Antonio , Di Lorenzo Francesca , Finocchiaro Susanna , Cimino Carolina , Telesca Rossana , Chiocchi Marcello , Sun Qiang , Wang Ying , Shi Yufang , Novelli Giuseppe , Melino Gerry 
Published: August 2021

AbstractWhile vaccination is the single most effective intervention to drastically reduce severe disease and death following SARS-CoV-2 infection, as shown in UK and Israel, some serious concerns have been raised for an unusual adverse drug reaction (ADR), including vaccine-induced immune thrombotic thrombocytopenia (VITT) with concurrent low platelets as well as capillary leak syndrome. In fact, the overall safety of the vaccine is highlighted by the low frequency of ADR considering that in UK, by the early June, 40 million first doses and 29 million second doses have been injected; nonetheless, 390 thrombotic events, including 71 fatal events have been reported. Interestingly, the cases reported low platelet counts with the presence of anti-platelet factor-4 (PF4) antibodies, indicating an abnormal clotting reaction. Here, out of three referred cases, we report a post-vaccine clinical case of fatal thrombosis with postmortem examination and whole exome sequencing (WES) analysis, whose pathogenesis appeared associated to a preexisting condition of thrombocytopenia due to myelodysplasia.
Population screening for glaucoma in UK: current recommendations and future directions
Authors: Hamid Sana , Desai Parul , Hysi Pirro , Burr Jennifer M. , Khawaja Anthony P. 
Published: August 2021

AbstractEffective population screening for glaucoma would enable earlier diagnosis and prevention of irreversible vision loss. The UK National Screening Committee (NSC) recently published a review that examined the viability, effectiveness and appropriateness of a population-based screening programme for primary open-angle glaucoma (POAG). In our article, we summarise the results of the review and discuss some future directions that may enable effective population screening for glaucoma in the future. Two key questions were addressed by the UK NSC review; is there a valid, accurate screening test for POAG, and does evidence exist that screening reduces morbidity from POAG compared with standard care. Six new studies were identified since the previous 2015 review. The review concluded that screening for glaucoma in adults is not recommended because there is no clear evidence for a sufficiently accurate screening test or for better outcomes with screening compared to current care. The next UK NSC review is due to be conducted in 2023. One challenge for POAG screening is that the relatively low disease prevalence results in too many false-positive referrals, even with an accurate test. In the future, targeted screening of a population subset with a higher prevalence of glaucoma may be effective. Recent developments in POAG polygenic risk prediction and deep learning image analysis offer potential avenues to identifying glaucoma-enriched sub-populations. Until such time, opportunistic case finding through General Ophthalmic Services remains the primary route for identification of glaucoma in the UK and greater public awareness of the service would be of benefit.
Coherent coupling between vortex bound states and magnetic impurities in 2D layered superconductors
Authors: Park Sunghun , Barrena Víctor , Mañas-Valero Samuel , Baldoví José J. , Fente Antón , Herrera Edwin , Mompeán Federico , García-Hernández Mar , Rubio Ángel , Coronado Eugenio , Guillamón Isabel , Yeyati Alfredo Levy , Suderow Hermann 
Published: August 2021

AbstractBound states in superconductors are expected to exhibit a spatially resolved electron-hole asymmetry which is the hallmark of their quantum nature. This asymmetry manifests as oscillations at the Fermi wavelength, which is usually tiny and thus washed out by thermal broadening or by scattering at defects. Here we demonstrate theoretically and confirm experimentally that, when coupled to magnetic impurities, bound states in a vortex core exhibit an emergent axial electron-hole asymmetry on a much longer scale, set by the coherence length. We study vortices in 2H-NbSe2 and in 2H-NbSe1.8S0.2 with magnetic impurities, characterizing these with detailed Hubbard-corrected density functional calculations. We find that the induced electron-hole imbalance depends on the band character of the superconducting material. Our results show that coupling between quantum bound states in superconductors is remarkably robust and has a strong influence in tunneling measurements.
Guided accumulation of active particles by topological design of a second-order skin effect
Authors: Palacios Lucas S. , Tchoumakov Serguei , Guix Maria , Pagonabarraga Ignacio , Sánchez Samuel , G. Grushin Adolfo 
Published: August 2021

AbstractCollective guidance of out-of-equilibrium systems without using external fields is a challenge of paramount importance in active matter, ranging from bacterial colonies to swarms of self-propelled particles. Designing strategies to guide active matter and exploiting enhanced diffusion associated to its motion will provide insights for application from sensing, drug delivery to water remediation. However, achieving directed motion without breaking detailed balance, for example by asymmetric topographical patterning, is challenging. Here we engineer a two-dimensional periodic topographical design with detailed balance in its unit cell where we observe spontaneous particle edge guidance and corner accumulation of self-propelled particles. This emergent behaviour is guaranteed by a second-order non-Hermitian skin effect, a topologically robust non-equilibrium phenomenon, that we use to dynamically break detailed balance. Our stochastic circuit model predicts, without fitting parameters, how guidance and accumulation can be controlled and enhanced by design: a device guides particles more efficiently if the topological invariant characterizing it is non-zero. Our work establishes a fruitful bridge between active and topological matter, and our design principles offer a blueprint to design devices that display spontaneous, robust and predictable guided motion and accumulation, guaranteed by out-of-equilibrium topology.
Mechanical metric for skeletal biomechanics derived from spectral analysis of stiffness matrix
Authors: Henyš Petr , Kuchař Michal , Hájek Petr , Hammer Niels 
Published: August 2021

AbstractA new metric for the quantitative and qualitative evaluation of bone stiffness is introduced. It is based on the spectral decomposition of stiffness matrix computed with finite element method. The here proposed metric is defined as an amplitude rescaled eigenvalues of stiffness matrix. The metric contains unique information on the principal stiffness of bone and reflects both bone shape and material properties. The metric was compared with anthropometrical measures and was tested for sex sensitivity on pelvis bone. Further, the smallest stiffness of pelvis was computed under a certain loading condition and analyzed with respect to sex and direction. The metric complements anthropometrical measures and provides a unique information about the smallest bone stiffness independent from the loading configuration and can be easily computed by state-of-the-art subject specified finite element algorithms.
Exploring tailor-made Brønsted acid sites in mesopores of tin oxide catalyst for β-alkoxy alcohol and amino alcohol syntheses
Authors: Manjunathan Pandian , Prasanna Varsha , Shanbhag Ganapati V. 
Published: August 2021

AbstractThe generation of Brønsted (Sn–OH) and Lewis (coordinatively unsaturated metal centers) acidic sites on the solid surface is a prime demand for catalytic applications. Mesoporous materials are widely employed as catalysts and supports owing to their different nature of acidic sites. Nevertheless, the procedure adopted to generate acid functionalities in these materials involves tedious steps. Herein, we report the tunable acidic sites containing Brønsted sites with relatively varied acid strength in tin oxide by employing soft template followed by simple thermal treatment at various temperatures. The readily accessible active sites, specifically Brønsted acidic sites distributed throughout the tin oxide framework as well as mesoporosity endow them to perform with exceptionally high efficiency for epoxide ring opening reactions with excellent reusability. These features promoted them to surpass stannosilicate catalysts for the epoxide ring opening reactions with alcohol as a nucleophile and the study was extended to aminolysis of epoxide with the amine. The existence of relatively greater acid strength and numbers in T-SnO2-350 catalyst boosts to produce a high amount of desired products over other tin oxide catalysts. The active sites responsible in mesoporous tin oxide for epoxide alcoholysis were studied by poisoning the Brønsted acidic sites in the catalyst using 2,6-lutidine as a probe molecule.
Changing characteristics of S. aureus bacteremia caused by PVL-negative, MRSA strain over 11 years
Authors: Yang Eunmi , Kim Eunsil , Chung Hyemin , Lee Yun Woo , Bae Seongman , Jung Jiwon , Kim Min Jae , Chong Yong Pil , Kim Sung-Han , Choi Sang-Ho , Lee Sang-Oh , Kim Yang Soo 
Published: August 2021

AbstractCommunity-acquired methicillin-resistant Staphylococcus aureus (MRSA) has emerged as an important cause of infection. We conducted a longitudinal study to evaluate changes in clinical and microbiological characteristics as well as outcomes of sequence type (ST) 72 MRSA bacteremia. We reviewed adult patients enrolled in a prospective cohort with ST72 MRSA bacteremia from August 2008 to December 2018 at Asan Medical Center, Seoul, South Korea. Changes in clinical characteristics, outcomes, and microbiological characteristics of patients over time were evaluated. Generalized linear and linear regression models were used to evaluate changes. Of the 1,760 isolates, 915 (62%) were MRSA bacteremia and 292 (31.9%) were ST72 MRSA. During the study period, the relative risk (RR) of MRSA bacteremia decreased annually by 3.7%; however, among MRSA bacteremia, RR of ST72 MRSA increased annually by 8.5%. Vancomycin minimum inhibitory concentration (MIC) decreased over the study period. Metastatic infection, persistent bacteremia, and recurrence of bacteremia within 12 weeks decreased significantly. There were no significant changes in 30-d and 12-week mortality. Antibiotic susceptibility of ST72 MRSA was evaluated, and the resistance rate to erythromycin decreased significantly. ST72 MRSA incidence increased annually; its vancomycin MIC and erythromycin resistance rate decreased over the 11 years.
Effect of sowing proportion on above- and below-ground competition in maize–soybean intercrops
Authors: Ren Yuanyuan , Zhang Li , Yan Minfei , Zhang Yanjun , Chen Yinglong , Palta Jairo A. , Zhang Suiqi 
Published: August 2021

AbstractThe relative contribution of above- and below-ground competition to crop yield under intercropping systems is critical to understanding the mechanisms of improved yield. Changes in the content of above- and below-ground biomass, leaf photosynthetic rate (Pn), leaf area index (LAI), chlorophyll meter reading (SPAD), diffuse non interceptance (DIFN), soil water storage (SWS), crop nitrogen (N), and phosphorus (P) uptake were examined in a 2-year trial of different maize–soybean intercropping systems on the Loess Plateau, China. Compared with the sole cropping system, shoot biomass of maize was increased by 54% in M2S2 and 62% in M2S4 strip intercropping treatment. The crop N and P uptake of maize increased significantly, by 54% and 50% in M2S2 and by 63% and 52% in M2S4 compared with their respective sole crop. LAI values of maize in intercropping systems were 14% and 15% for M2S2 and M2S4 less than that in the sole crop. The DIFN of intercropped maize was increased by 41% and 48% for M2S2 and M2S4 compared to monocrop. There were no significant differences in Pn and SWS in both crops between the two cropping systems. The contribution rate of DIFN in M2S2 and crop P uptake in M2S4 on the biological yield in intercropping system was the highest among all factors. We conclude that the sowing proportion affects above- and below-ground competition in maize–soybean intercropping systems.