bluuebunny/crossref_metadata_2025_split huggingface dataset:
فعالية تطبيق الطريقة المباشرة في رفع كفاءة المحادثة لدى تلاميذ المدرسة العالية التابعة لمعهد السلطان حسن الدين في ولاية غووا
Authors: Hakim Azizul , Adhayani Sri 
Published: January 2021

ملخ ص الغرض لهذا البحث العلمي هو تحليل مدى فعالية تطبيق الطريقة المباشرة في رفع كفاءة المحادثة لدى تلاميذ المدرسة العالية التابعة لمعهد السلطان حسن الدين في ولاية غووا. واستخدم الباحثان طريقة البحث التجريبـي، وطريقة جمع البيانات وطريقة تحليل البيانات؛ ففي جمع البيانات استخدما الطريقة الوثيقية والاختبار القبلي والمعاملة والاختبار البعدي والمراقبة؛ وفي تحليل البيانات استخدم الباحثان الطريقة الإحصائية الوصفية. ويستفاد من نتائج البحث أن تطبيق الطريقة المباشرة في تدريس اللغة العربية فعال في رفع كفاءة المحادثة لدى التلاميذ حيث أن تحصيل دراستهم في الاختبار البعدي يزداد ارتقاء بمقارنة اختبارهم القبلي. ويجوز الاستنتاج أن تطبيق الطريقة المباشرة فعال في رفع كفاءة المحادثة لدى تلاميذ المدرسة العالية التابعة لمعهد السلطان حسن الدين في ولاية غووا Abstrak:Tujuan penelitian ilmiah ini adalah menganalisis efektivitas penerapan metode langsung dalam meningkatkan kemampuan bercakap siswa Madrasah Aliyah Pondok Pesantren Sultan Hasanuddin Kabupaten Gowa. Peneliti menggunakan metode penelitian eksperimen, metode pengumpulan data dan metode analisis data. Dalam mengumpulkan data, peneliti menggunakan metode dokumentasi, tes awal, perlakuan, tes akhir, dan observasi. Dalam menganalisis data, peneliti menggunakan metode statistik deskriptif. Dari hasil penelitian diperoleh bahwa penerapan metode langsung dalam mengajarkan bahasa Arab, efektif dalam meningkatkan kemampuan bercakap siswa di mana hasil belajar mereka pada tes akhir meningkat dibandingkan dengan tes awal. Dari hasil tersebut dapat disimpulkan bahwa penerapan metode langsung, efektif dalam meningkatkan kemampuan bercakap siswa Madrasah Aliyah Pondok Pesantren Sultan Hasanuddin Kabupaten Gowa.
THE IMPLEMENTATION OF THE LEARNING CYCLE MODEL FOR THE STUDENTS’ LEARNING OUTCOMES IN THE RESEARCH METHODOLOGY
Authors: Anas Muh. , Prasetyo Moh. Mulyadi 
Published: January 2021

Abstract:This study aims to determine the application of the Learning Cycle model to the students’ learning outcomes of the Research Methodology subjects in the Biology Education department at STKIP Pembangunan Indonesia. This research is a quasi-experimental study with a pretest-posttest control group design. The sample used was 30 students of the Biology department at STKIP Pembangunan Indonesia who programmed Research Methodology. The instruments used were observation sheets and 10 numbers of essay questions. The data analysis techniques are the descriptive statistical analysis and the inferential statistical analysis with the independent sample t-test. Based on the inferential analysis using the independent sample t-test where the p-value of 0.020 is smaller than 0.05 (p <0.05), it is found that there is an effect of the Learning Cycle model on the students’ learning outcomes. Based on the findings of the descriptive statistical analysis, it can be concluded that the application of the Learning Cycle model has an effect on the students’ learning outcomes at STKIP Pembangunan Indonesia in the Research Methodology subject with the value range of 85 – 100 of 6 students with the percentage of 20% and the value range of 65 – 84 of 24 students with the percentage of 80%.Abstrak:Penelitian ini bertujuan untuk mengetahui penerapan model pembelajaran Learning Cycle terhadap hasil belajar mata kuliah Metodologi Penelitian mahasiswa Pendidikan Biologi STKIP Pembangunan Indonesia. Penelitian ini merupakan penelitian Quasi Experimental dengan desain penelitian Pretest-Posttest Control Group Design. Sampel yang digunakan adalah 30 mahasiswa jurusan Biologi STKIP Pembangunan Indonesia yang memprogramkan mata kuliah Metodologi Penelitian. Instrumen yang digunakan adalah lembar observasi dan 10 nomor soal esay. Teknik analisis data yang digunakan adalah analisis statistik deskriptif dan analisis statistik inferensial dengan uji Independent Sampel t-test. Berdasarkan hasil analisis inferensial menggunakan uji Independent Sampel t-test diperoleh nilai p sebesar 0,020 yang lebih kecil dari 0,05 (p<0,05) dengan demikian dapat disimpulkan bahwa ada pengaruh penerapan model pembelajaran Learning Cycle terhadap hasil belajar mahasiswa. Berdasarkan hasil analisis statistic deskriptif dapat disimpulkan bahwa penerapan model pembelajan Learning Cycle mampu meningkatkan hasil belajar mahasiswa STKIP Pembangunan Indonesia pada mata kuliah metodologi penelitian dengan rentang nilai 85 – 100 sebanyak 6 mahasiswa dengan persentase 20% dan rentang nilai 65 – 84 sebanyak 24 mahasiswa dengan persentase 80%.
تنفيذ تعليم الشخصية دراسة تحليلية في معهد العناية باندونج
Authors: Islamy Mohammad Rindu Fajar , Supriadi Udin , Rojak Rama Wijaya Abdul , Romli Usup , Anwar Saepul , Parhan Muhamad 
Published: January 2021

ملخ ص يواجه عالم التعليم اليوم مجموعة متنوعة من المشكلات الجديدة التي تتطور بشكل جديد، وهذا بالطبع يمثل تحديًللعلماء المتخصصة ف التعليم والنشطاء التربويين للعثور على أنماط تعليم شخصية متميزة. تهدف هذه بحث الرسالة العلمي ة إلى استكشاف تنفيذ تعليم الشخصية كمحاولة لمنع الإخلال بأخلاق الطلاب. إن تنمية القيم الروحية التي تتماشى مع تعاليم الإسلام أمر أساسي ووسيل ة ف بناء أخلاق وحضارة الأم ة الإسلامي ة. باستخدام الأساليب المتنوعة ، تركزت الدراسة ف مدرسة العناية الإسلامية من خلال استكشاف البيانات اللازمة من خلال تقنيات المقابلة والملاحظة والتحليل التفاعلي. تظهر الدراسة أن نجاح الأنشطة التربوية ف بناء الأخلاق يجب أن يتكامل مع عدة مكونات أساس ية وهي المناهج التعليمي ة وطرق التدريس وتقوي ة برامج نشاط التعلم ف تقوي ة الشخصية الإسلاميةAbstrak Dunia pengajaran saat ini menghadapi berbagai problem baru yang terus berkembang. Tentu saja, ini menjadi tantangan bagi para ilmuan dalam pengajaran dan aktivitas pendidikan untuk dapat menemukan tipe pengajaran kepribadian yang baik. Penelitian ini bertujuan mengungkap penerapan pengajaran kepribadian sebagai usaha untuk memerangi kerusakan akhlak pada siswa. Pengembangan nilainilai rohani yang sejalan dengan ajaran Islam adalah hal yang mendasar sebagai instrumen dalam membangun akhlak dan peradaban umat Islam. Dengan berbagai metode, penelitian di Madrasah al-Inayah al-Islamiyah dilakukan dengan menyingkap data-data primer melalui teknik wawancara, observasi, dan analisis interaktif. Penelitian ini menemukan, bahwa keberhasilan aktivitas pendidikan dalam membangun akhlak, harus terintegrasi dengan beberapa komponen pokok, yaitu kurikulum, metode pengajaran, penguatan program kegiatan pembelajaran, demi memperkuat kepribadian Islam siswa.
INTERNATIONALIZATION OF ISLAM RAHMATAN LIL 'ALAMIN THROUGH PENGURUS CABANG ISTIMEWA NAHDLATUL ULAMA (PCINU): MOTIVATION AND CONTRIBUTION
Authors: Purwono Andi 
Published: January 2021

Sebagai organisasi keagamaan terbesar, Nahdlatul Ulama memiliki peran internasional bahkan sejak awal pendiriannya. Salah satu peran itu diwujudkan melalui pembentukan Pengurus Cabang Istimewa di luar negeri. Artikel ini bertujuan menjelaskan alasan pendiriannya serta mendeskripsikan aktivitas dan kontribusinya bagi dunia maupun politik luar negeri Indonesia. Penelitian kualitatif ini menggunakan metode pengumpulan data studi pustaka ditambah wawancara. Penelitian menemukan bahwa motivasi ideasional terkait keterpanggilan untuk menyebarkan ajaran Islam rahmatan lil ‘alamin menjadi alasan pendirian. Aktivitas menjelaskan wajah Islam sebenarnya yang memberi kontribusi positif baik dalam kerangka hubungan Islam dan Barat maupun bagi politik luar negeri Indonesia.
Fairy-tale motifs in a translation of the film adaptation of the literary work Modrý lampáš (Das Blaue Licht)
Authors: Zahorák Andrej 
Published: January 2021

Abstract
The paper deals with the translation of the film adaptation of the literary work Das Blaue Licht (‘The Blue Light’), primarily intended for child recipients. The aim of this paper is to present a conceptual framework to describe specific aspects of communication-translation (translating children’s and youth literature, aspects of age and the projection of ideas in a different mode of adaptation), and subsequently provide a comprehensive evaluation related to the optimality of the chosen translation procedures and strategies in the Slovak dubbed version of the above-mentioned audiovisual work. The focus is on how the semantic and expressive function of the original is preserved, taking into account the way linguistic reality is depicted in communication with the child recipient.
Current application of exosomes in medicine
Authors: Bryl Rut , Borowiec Blanka , Siroma Rafael Shinoske , Pinto Nelson , Melo Marcelo A. , Shibli Jamil A. , Dyszkiewicz-Konwińska Marta 
Published: January 2021

Abstract
Exosomes belong to structures called extracellular vesicles (EVs). These spherical units, secreted by most eukaryotic cells, attracted significant interest among researchers in recent years. Exosomes undergo secretion from almost all types of mammalian cells, including dendritic cells, B cells, epithelial cells, mastocytes, reticulocytes, platelets, T cells, mesenchymal stem cells, adipocytes, bone marrow-derived stem cells, embryonic stem cells, fibroblasts, cardiac myocytes, endothelial cells, oligodendrocytes, astrocytes, microglia, neurons, neural stem cells, hepatocytes, lung spheroid cells, as well as tumor cells. Exosomes have several features that enable many methods of their isolation from biological material. Furthermore, physicochemical properties such as size, mass, density, or the ability to interact with specific proteins allowed for the development and advance of several effective methods. Work on exosomes’ recovery and purity made it possible to most effectively determine their isolation methods’ efficiency and accuracy. A common ground for the researchers’ interest in exosomal analyses is the role of exosomes as carriers of disease biomarkers. It has been suggested that exosomes can be used in vaccine development and other immunological-related purposes, as one of their characteristics is the ability to present antigens. Moreover, exosomes have a long half-life. As the human body does not perceive them as foreign bodies, they can penetrate cell membranes and target specific cells, making them even better candidates for the applications mentioned above. Therefore, the following review deals with the nature of exosomes, as well as various methods of their isolation and use in medicine.

Running title: Current application of exosomes in medicine
The extracellular reactive oxygen species levels in primary in vitro culture of human ovarian granulosa and cumulus cells
Authors: Kocherova Ievgeniia , Bryl Rut , Crha Igor , Ventruba Pavel , Zakova Jana , Ješeta Michal 
Published: January 2021

Abstract
In the female reproductive tract, reactive oxygen species (ROS) may exert physiological and pathophysio-logical effects. Although ROS play an essential role as the signaling molecules, their excessive accumulation contributes to the pathogenesis of many reproductive processes. In the ovarian follicle, ROS affect multiple physiological processes, including oocyte maturation and fertilization. However, a lack of studies showing to which extend ovarian granulosa and cumulus cells can contribute to the development of oxidative stress within the ovarian follicle. In the presented research, the extracellular ROS accumulation level was investigated using GCs and CCs primary in vitro cultures. The obtained results demonstrated a steady decrease in extracellular ROS level during GCs primary culture. By contrast, ROS concentration in CCs conditioned medium increased gradually between the first and the seventh days of culture. The observed changes may reflect the proliferation status and metabolic activity of GCs and CCs during in vitro culture. Additionally, the elevated ROS level at respective points of time could occur as a consequence of culture in atmospheric oxygen. The distinct function and localization within the ovarian follicle may explain the differences between GCs and CCs oxygen metabolism.

Running title: Reactive oxygen species in primary culture of human follicular cells
Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells
Authors: Borowiec Blanka , Bryl Rut , Bryja Artur , Mozdziak Paul , Dyszkiewicz-Konwińska Marta 
Published: January 2021

Abstract
Several genes, namely CD44, CD90, CD105 and PCNA may be important in differentiation of porcine mucosa cell cultures. These genes are, inter alia, responsible for cell adhesion to extracellular matrix and its constituent secretion, cytoskeleton organization, epithelial to mesenchymal transition or proper course of DNA replication. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to produce buccal mucosa cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of CD44, CD90, CD105 and PCNA were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. CD44, CD90 and PCNA showed an unchanged expression pattern. Expression of CD44 on day 7 was the highest of all factors measured. The greatest difference between the measurement on 7th and 30th day was found in the PCNA gene. These results broaden the understanding of the transcriptome changes in porcine buccal mucosa cells for the duration of in vitro cultivation. Nevertheless, it is very important to consider that the in vitro conditions do not fully reflect the changes taking place in the living organism. It appears that tissues of the oral cavity possess high regenerative potential, and constitute suitable model for wound healing investigation.

Running title: Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells
Current stem cells technologies used in medicine
Authors: Müller Maria , Czarnecka Julia , Brzeziński Michał , Prus Jakub , Kulak Bartosz , Hołubowski Andrzej , Stasiak Mateusz , Borowiec Blanka , Bryl Rut , Moncrieff Lisa , Dyszkiewicz-Konwińska Marta 
Published: January 2021

Abstract
Stem cells and their usage for a long time are thought to be the future and hope in modern medicine. In this review we summarize development in science and bioengineering in this field. Opening with a description of newly discovered and studied sources of stem cells acquisition we present scientific methods progress and their application like 3D printing or transdifferentiation mode of action and results of these techniques. Technologies of genome editing like transcription activator-like effector nuclease, zinc-finger nucleases, or CRISPR Cas9 are also presented. In disease treatment and tissue reconstruction stem cells have proved to be effective most times due to great proliferation and differentiation potentials in presented in this summary pre-clinical and clinical studies for diseases like peripheral nerve palsy, myocardial infarction and heart ischemic disease and corneal wound healing.

Running title: Current stem cells technologies used in medicine
Exosomes and their possible applications in the management of gestational diabetes
Authors: Sibiak Rafał , Jaworski Michał , Barrett Saoirse , Bryl Rut , Gutaj Paweł , Wender-Ożegowska Ewa 
Published: January 2021

Abstract
Gestational diabetes mellitus (GDM) is thought to be the most common metabolic gestational complication. Its prevalence has been continuously increasing in recent decades along with the rising epidemic of obesity in modern societies. GDM is associated with an increased risk of fetal growth abnormalities, birth traumas, and several neonatal complications. Widely available screening tools, fasting glucose measurements, combined with oral glucose tolerance test results, contribute to the reduction of the risk of those complications. Nevertheless, we are still looking for novel reliable early markers of GDM. It has been established that high 1st-trimester exosome concentrations could predispose the development of GDM in later pregnancy. Exosomes can be easily isolated from various tissues and body fluids in pregnant patients. Due to this, extracellular vesicle concentration assessment appears as a new promising tool in the prediction of GDM at the preclinical stage of the disease. Furthermore, it has been found that women already diagnosed with GDM have significantly higher exosome concentrations compared with healthy individuals. These findings could help to elucidate the molecular pathogenesis of GDM. Exosomes are loaded with various molecules especially proteins, lipids, mRNAs, and microRNAs. Altered expression of numerous microRNAs and enzymes such as dipeptidyl peptidase-IV in exosomes isolated from patients with GDM may suggest their direct contribution to the mechanisms of glucose intolerance. This knowledge could be used in the development of new therapeutic strategies in patients with GDM. Nevertheless, it should be emphasized that these are only preliminary results that require further investigations.

Running title: Exosomes in gestational diabetes
Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture
Authors: Bryja Artur , Pieńkowski Wojciech , Stefańska Katarzyna , Chermuła Błażej , Bryl Rut , Wieczorkiewicz Maria , Kulus Jakub , Wąsiatycz Grzegorz , Bukowska Dorota , Ratajczak Kornel , Jaśkowski Jędrzej M. , Petitte James N. , Mozdziak Paul , Pawelczyk Leszek , Spaczyński Robert Z. , Antosik Paweł 
Published: January 2021

Abstract
The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.
These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.
Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.
Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.
Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.

Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells
The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study
Authors: Bryl Rut , Dompe Claudia , Jankowski Maurycy , Stefańska Katarzyna , Narenji Afsaneh Golkar , Kulus Jakub , Kulus Magdalena , Wieczorkiewicz Maria , Wąsiatycz Grzegorz , Jaśkowski Jędrzej M. , Kaczmarek Mariusz , Petitte James N. , Mozdziak Paul , Antosik Paweł , Bukowska Dorota 
Published: January 2021

Abstract
ADSCs are readily accessible and widely available. Isolated through a minimally invasive procedure from adipose depots, they can be found at diverse body location, where they served various functions, including energy homeostasis. They can be obtained upon surgeries from otherwise waste tissues, like after excision of fat tissue or liposuction. In addition, due to the possibility to isolate many ADSCs, in vitro proliferation can be performed in a short time period, resulting in cells showing more predictable results[1]. For this study ADSCs were obtained from waste material following routing sterilization procedures of dogs. This study aimed to analyse the expression of MSC specific markers before and after in vitro differentiation of ASCs. Three positive and three negative markers were analysed, CD105, CD73, CD90, CD34, CD14 and CD45. There were significant differences detected in the expression of all of the genes, with most of them exhibiting notable downregulation. The only exception, CD14 showed major upregulation after the process of differentiation. These changes confirm the success of differentiation, as well as suggest that this process significantly lowers the stem-like ability of ASCs. This knowledge should serve as a reference for further molecular and clinical studies, possibly aiding the understanding of the internal mechanisms governing the differentiation and stemness of ASCs, to enable their widespread and safe application in regenerative medicine.

Running title: Mesenchymal markers during ASC osteogenic differentiation
Human placenta-derived stem cells - recent findings based on the molecular science
Authors: Sibiak Rafał , Jaworski Michał , Dorna Zuzanna , Pieńkowski Wojciech , Stefańska Katarzyna , Bryl Rut , Žáková Jana , Crha Igor , Ventruba Pavel , Ješeta Michal , Kempisty Bartosz 
Published: January 2021

Abstract
The human placenta is a complex, multifunctional transient fetomaternal organ. The placenta is composed of the maternal decidua basalis and its fetal part, consisting of the mesenchymal and trophoblast cell lineages. Both the placenta and the amniotic membranes are abundant in readily available placenta-derived mesenchymal stem cells (PD-MSCs). The clinical application of the PD-MSCs opens new perspectives for regenerative medicine and the treatment of various degenerative disorders. Their properties depend on their paracrine activity – the secretion of the anti-inflammatory cytokines and specific exosomes. In contrast to the PD-MSCs, the trophoblast stem cells (TSCs) are much more elusive. They can only be isolated from the blastocyst-stage embryos or the first-trimester placental tissue, making that procedure quite demanding. Also, other cultures require specific, strictly controlled conditions. TSCs may be potentially used as an in vitro model of various placental pathologies, facilitating the elucidation of their mysterious pathogenesis and creating the environment for testing the new drug efficiency. Nonetheless, it is unlikely that they could be ever implemented as a part of novel cellular therapeutic strategies in humans.

Running title: Current knowledge on the placental stem cells
Comparison of microfluidic and swim-up sperm separation methods for IVF
Authors: Ješeta Michal , Franzová Kateřina , Žáková Jana , Ventruba Pavel , Crha Igor 
Published: January 2021

Abstract
Sperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been applied more and more frequently in recent years. This method is often presented as extremely gentle to spermatozoa and decreasing significantly concentration of sperm cells with fragmented DNA when compared to conventional methods. The aim of our study was to verify a microfluidic chip system from the perspective of its potential to select spermatozoa with non-fragmented DNA. We tested the efficiency of this separation method against the swim-up method. In this study we evaluated sperm DNA integrity before and after the separation methods in ten patients. Ejaculate of each patient was separated by both the swim up method and the microfluidic chip method at the same time. It was shown that both the methods are very similar in reduction of spermatozoa with fragmented DNA. Interestingly, the concentration of spermatozoa with fragmented DNA was lower after the microfluidic separation than after the swim-up method in all the patients. Nevertheless, the differences were not statistically significant with only 2.1% on average, which is negligible in terms of practical use.

Running title: Microfluidic chip and DNA fragmentation