bluuebunny/crossref_metadata_2025_split huggingface dataset:
According to the issue of the giant freshwater prawn ((<i>Macrobrachium rosenbergii</i>)
Authors: Shumeyko D. , Klochko E. , Nazina Y. , Manafova S. , Orlova T. 
Published: July 2021

Purpose: study the biotechnical features of rearing juvenile giant freshwater prawn (Macrobrachium rosenbergii) using recirculating aquaculture systems (RAS).Materials and methods. Two fish-farming modules RAS and aquarian complex were used for the work. One of them has a total volume of 6.5 m3, the second - 14.5 m3. Larvae were reared in gasite cages of 0.021 m3 in an aquarian complex of two 200-liter aquariums. Larvae of one female prawn were used in an amount of 8500 units. At each stage of prawns' development we used different versions of feed of both animal and plant origin: artemia, minced fish, boiled chicken eggs (protein + yolk), beef liver and oat flakes.Results. Brood fish were kept at an average planting density of 6.2 individuals/m2, with a sex ratio of 1:4.5 (males: females). The planting density of larvae in cages was 2,125 units/cage or 101.2 units/liter. The average weight of juveniles during rearing of postlarvae at 130 days was 3.19 g. Juvenile yield was 49.9%. Specific growth rate of biomass varied from 4.87 to 3.68 %. Planting density of juveniles varied from 1775,6 units/m2 to 660,9 units/m2. As a result, the feeding coefficient was 5.24 units. Growing productivity was 362.7 pcs/m2 or 1156.8 g/m2 in the RAS module.Conclusion. There were established biotechnical peculiarities and production possibilities of growing young giant freshwater prawn (Macrobrachium rosenbergii) using fish-breeding RAS and aquarian complex. Modernization of fish-feeding RAS and use of some recommendations on feeding suggest the possibility of their use for breeding and rearing of juvenile giant freshwater prawns in already existing warm-water enterprises, initially aimed at other objects of aquaculture.
Analisa kebutuhan platform pendorpowermassage.id sebagai solusi bagi para lulusan sarjana pendidikan kepelatihan olahraga
Authors: Adila Fekie , Muhibbi Muhammad 
Published: July 2021

Prospek peluang kerja yang dimiliki oleh mahasiswa Program Studi Pendidikan Kepelatihan Olahraga cukup menjanjikan antara lain pelatih, private trainer, instruktur olahraga, sport therapist, manajer lembaga atau klub olahraga, sport event organizer, dan tenaga pendidik jasmani. Akan tetapi hal ini berbanding terbalik di lapangan, banyak alumni lulusan S1 Pendidikan Kepelatihan Olahraga bekerja tidak sesuai bidangnya. Penelitian ini bertujuan untuk mengetahui kebutuhan lulusan S1 Pendidikan Kepelatihan Olahraga Universitas Riau terhadap pengadaan Platform PENDORPOWERMASSAGE.ID (PPM.ID). Metode penelitian yang digunakan adalah metode deskriptif. Subjek yang terlibat dalam penelitian ini berjumlah 24 orang yang diambil dari 20 persen dari jumlah total mahasiswa Pendidikan Kepelatihan Olahraga angkatan 2016. Pengumpulan data menggunakan angket google form. Analisis data menggunakan statisitik deskriptif. Hasil dari penelitian ini subyek membutuhkan lapangan pekerjaan setelah lulus kuliah; subyek siap terjun ke lapangan untuk menjadi masseur dan menangani cedera; subyek butuh aplikasi software untuk sport massage, subjek memerlukan standarisasi dan training dari penyedia platform PPM.ID massage; subjek memerlukan adanya standarisasi harga jasa massage dari penyedia platform. Kesimpulan dari penelitian ini perlunya pengadaan platform PPM.ID pada Program Studi Pendidikan Kepelatihan Olahraga
Investigation of Dry Eye Symptoms in Health Workers of National Medical College and Teaching Hospital following Corona Virus Pandemic
Authors: Bista Padam Raj , Bista Bipin , Mishra Surendra , Yadav Reena , Chaudhary Sushma , Gupta Sharad 
Published: July 2021

Background: With the outspread of global pandemic Corona virus disease 19. (COVID-19), use of face masks was taken as a vital deed against severe acute respiratory syndrome coronavirus-2. (SARS-CoV-2) The aim of this study was to assess symptoms of mask associated dry eye (MADE) among health workers.
Materials and Methods: A total of 169 heath workers meeting the inclusion criteria were selected by simple random sampling from respective department. Data were obtained by providing with questionnaire established by the researcher and Ocular Surface Disease Index (OSDI) to the participants from November 1, 2020 to March 31, 2021 at National Medical College and Teaching Hospital. The data were evaluated using correlation test.
Results: A total of 169 heath workers were enrolled in the study. The mean age of the patient was 31.74±7.34 years (range 19-56).According to OSDI, 27(15.9%) of the participants had mild dry eye symptoms followed by 15 (8.87%) cases of moderate and 44(26.03%) of severe respectively. Duration of mask wearer and electronic devices usage were two variables that were statistically correlated with OSDI (p<0.01). The OSDI was not statistically correlated with age, glass wear and sleep duration.
Conclusion: With the emergence of COVID -19, measures to cope against this pandemic are must. With the increasing use of controlling measures, it is prime responsibility of ophthalmic faculty to start awareness regarding proper use of these anti-COVID measures and make sustainable environment in future.
Pengaruh Kemandirian Belajar Siswa Terhadap Hasil Belajar Matematika
Authors: Indah Ratna Puspita , Farida Anisatul 
Published: July 2021

The study aimed to identify the influence of independence for the mathematicsÂ student learning in SMP Al Irsyad Surakarta. The subject of this research are all students of class 8 SMP Al Irsyad Surakarta, which consisted of 120 students and the samples chosenÂ with random sampling are 49 students in 3 class in the school year 2020/2021. SamplesÂ were taken using the Random sampling technique. The data of independence for the mathematics student learning using a questionnaire. This study using quasi experimental research. The prerequisite using the normality test and linearity test. The normality test by using Kolmogorov Smirnov and the linearity test by using Anova. When the a prerequisite fulfilled, so followed by the Linear Regression. The result showed there are significant impact study of independent learning. Independent learning have a positive influence of mathematics students, although the result is still below 50%. This may be affected by the lack of knowledge students learn about independence, so not been undertaken in full. The conclusions obtained that students could be more improve mathematics learning outcomes.Â Keywords: learning, independent learning, mathematics, learning outcomes
Complete section of the left vagus nerve does not preclude the efficacy of vagus nerve stimulation: illustrative case
Authors: Noris Alice , Roncon Paolo , Peraio Simone , Zicca Anna , Lenge Matteo , Di Rita Andrea , Genitori Lorenzo , Giordano Flavio 
Published: July 2021

BACKGROUND
Vagus nerve stimulation (VNS) represents a valid therapeutic option for patients with medically intractable seizures who are not candidates for epilepsy surgery. Even when complete section of the nerve occurs, stimulation applied cranially to the involved nerve segment does not preclude the efficacy of VNS. Complete vagus nerve section with neuroma causing definitive left vocal cord palsy has never been previously reported in the literature.

OBSERVATIONS
Eight years after VNS implant, the patient experienced worsening of seizures; the interrogation of the generator revealed high impedance requiring surgical revision. On surgical exploration, complete left vagus nerve section and a neuroma were found. Vocal cord atrophy was found at immediate postoperative laryngeal inspection as a confirmation of a longstanding lesion. Both of these events might have been caused by direct nerve injury during VNS surgery, and they presented in a delayed fashion.

LESSONS
VNS surgery may be complicated by direct damage to the left vagus nerve, resulting in permanent neurological deficits. A complete section of the nerve also enables an efficacious stimulation if applied cranially to the involved segment. Laryngeal examination should be routinely performed before each VNS surgery to rule out preexisting vocal cord dysfunction.
Cerebral vasculopathy and strokes in a child with COVID-19 antibodies: illustrative case
Authors: Foster Chase H. , Vargas Anthony J. , Wells Elizabeth , Keating Robert F. , Magge Suresh N. 
Published: July 2021

BACKGROUND
The ability of coronavirus disease 2019 (COVID-19) to cause neurological insults in afflicted adults is becoming increasingly understood by way of an ever-growing amount of international data. By contrast, the pandemic illness’s neurological effects in the pediatric population are both poorly understood and sparsely reported.

OBSERVATIONS
In this case, the authors reported their experience with a preschool-age child with hydrocephalus who suffered multiterritory strokes presumed secondary to immune-mediated cerebral vasculopathy as a result of asymptomatic COVID-19 infection.

LESSONS
Growing evidence indicates that COVID-19 can cause neurological sequelae such as encephalitis and strokes. In this case report, the authors discussed a case of cerebral vasculopathy and strokes in a pediatric patient who was positive for COVID-19.
Stereotactic surgery for neurocysticercosis of the 4th ventricle: illustrative cases
Authors: Saavedra Luis J. , Vásquez Carlos M. , García Hector H. , Antonio Luis A. , Caucha Yelimer , Félix Jesús , Medina Jorge E. , Lines William W. 
Published: July 2021

BACKGROUND
Neurocysticercosis, caused by the larval stage of Taenia solium, affects the cerebral ventricles in 20–30% of cases and may lead to hydrocephalus and other neurological morbidity. Conventional treatment for cysts in the 4th ventricle includes open surgery (suboccipital approach) and neuroendoscopy, with the latter being the option of choice. Stereotactic surgery, minimally invasive, offers a good alternative for this type of deep lesion.

OBSERVATIONS
The authors report the cases of two women, 30 and 45 years old, who presented with headache, dizziness, and ataxia and were diagnosed with 4th ventricle cysticercosis. Magnetic resonance imaging (MRI) revealed dilated 4th ventricles (approximately 2.5 cm in both cases, with cystic images inside the ventricular cavity). Both patients were treated with stereotactic surgery via a suboccipital transcerebellar approach. Cyst material was extracted, and the diagnosis was confirmed by pathological examination. The surgeries had no complications and resulted in clinical improvement. Control MRI scans showed reduction of the volume of the ventricle without residual cysts.

LESSONS
Minimally invasive stereotactic surgery provided a safe alternative for 4th ventricle neurocysticercosis cysts, with more benefits than risks in comparison with conventional techniques.
EXTRATOS VEGETAIS NO CONTROLE DO FUNGO FUSARIUM OXYSPORUM E SEU EFEITO SOBRE FUNGOS MICORRÍZICOS ARBUSCULARES EM PLANTAS DE MILHO
Authors: Domingos Mariana Moreira , Melloni Rogério , Ferreira Gustavo Magno dos Reis 
Published: July 2021

Com papel importante na agroecologia, extratos vegetais têm sido propostos como método alternativo aos agrotóxicos no controle de fitopatógenos, principalmente os do gênero Fusarium, mas questiona-se o seu efeito sobre fungos benéficos formadores de micorriza. Diante disso, o objetivo desse estudo foi compreender o efeito de extratos vegetais aquosos de Mentha spicata, Tagetes patula, Cymbopogon nardus e Allium sativum no crescimento micelial de Fusarium oxysporum e na formação de micorriza, por diferentes espécies de fungos micorrízicos arbusculares (FMAs), em condições controladas. Para a avaliação da atividade antifúngica dos extratos em F. oxysporum, prepararam-se os extratos aquosos nas concentrações de 0 e 10%, com posterior incorporação em meio de cultura BDA. Para análise da influência sobre a formação de micorriza, foram testadas as concentrações de 2,5; 5,0; 7,5 e 10,0% do extrato aquoso de Allium, espécie de maior atividade antifúngica preliminar. Todas as concentrações apresentaram efeito negativo na formação de micorriza, em milho inoculado com Gigaspora rosea ou Rhizophagus clarus, principalmente para a primeira espécie de FMA.
Avaliação da qualidade do solo em manejo agroflorestal utilizando a cromatografia circular de Pfeiffer e indicadores microbiológicos
Authors: Razera Raissa , Vaz Adriel Rodrigues , Bastos Aline Cipriano Valentim , Duarte Iolanda Cristina Silveira , Franco Fernando Silveira 
Published: July 2021

A Cromatografia Circular de Pfeiffer (CCP) é um indicador qualitativo do solo, de baixo custo, acessível e tem mostrado grande potencial nos estudos em Agroecologia. O objetivo foi analisar a qualidade do solo de um Sistema Agroflorestal com diferentes formas de manejo, durante o período de 12 meses, utilizando indicadores microbiológicos e parâmetros químicos do solo, correlacionados com a CCP. As amostras de solo, coletadas nas estações seca e chuvosa, foram analisadas utilizando a CCP e os seguintes indicadores: bactérias heterotróficas mesófilas totais, fungos totais, pH, umidade e matéria orgânica. Constatou-se que não houve diferença significativa entre o SAF controle e o SAF manejado, no período estudado, para todos os indicadores analisados. A CCP, as bactérias heterotróficas mesófilas totais e os fungos totais mostraram-se sensíveis a sazonalidades entre as estações seca e chuvosa. Houve correlação positiva, demonstrado pela zona de aeração da CCP, com matéria orgânica e umidade do solo. Conclui-se que a intensidade do manejo aplicado no SAF não foi suficiente para causar mudanças significativas na qualidade do solo, durante o período avaliado
A Smartphone-Fluidic Digital Imaging Analysis System for Pancreatic Islet Mass Quantification
Authors: Yu Xiaoyu , Zhang Pu , He Yi , Lin Emily , Ai Huiwang , Ramasubramanian Melur K. , Wang Yong , Xing Yuan , Oberholzer José 
Published: July 2021

Islet beta-cell viability, function, and mass are three decisive attributes that determine the efficacy of human islet transplantation for type 1 diabetes mellitus (T1DM) patients. Islet mass is commonly assessed manually, which often leads to error and bias. Digital imaging analysis (DIA) system has shown its potential as an alternative, but it has some associated limitations. In this study, a Smartphone-Fluidic Digital Imaging Analysis (SFDIA) System, which incorporates microfluidic techniques and Python-based video processing software, was developed for islet mass assessment. We quantified islets by tracking multiple moving islets in a microfluidic channel using the SFDIA system, and we achieved a relatively consistent result. The counts from the SFDIA and manual counting showed an average difference of 2.91 ± 1.50%. Furthermore, our software can analyze and extract key human islet mass parameters, including quantity, size, volume, IEq, morphology, and purity, which are not fully obtainable from traditional manual counting methods. Using SFDIA on a representative islet sample, we measured an average diameter of 99.88 ± 53.91 µm, an average circularity of 0.591 ± 0.133, and an average solidity of 0.853 ± 0.107.Viaanalysis of dithizone-stained islets using SFDIA, we found that a higher islet tissue percentage is associated with top-layer islets as opposed to middle-layer islets (0.735 ± 0.213 and 0.576 ± 0.223, respectively). Our results indicate that the SFDIA system can potentially be used as a multi-parameter islet mass assay that is superior in accuracy and consistency, when compared to conventional manual techniques.
Vertical Transmission and Discordance of Cytomegalovirus in Twin Pregnancies
Authors: Hutton Jill , Rowan Paul J. 
Published: July 2021

ObjectiveThe objectives are to estimate the vertical transmission rate in twins relative to singleton pregnancies, to evaluate whether discordance within twin pairs is rare, and to characterize concordance within monozygotic and dizygotic twin pairs in relation to hereditability.MethodsWe first sought to estimate the vertical transmission rate of congenital CMV infection in twins by gathering cohort-based studies of congenital CMV in which vertical transmission in both singleton and twin pregnancies was reported. This also allowed us to compare singleton and twin infection rates. From the above studies and other large cohorts of congenitally infected infants, the percentage of discordantly infected twin pairs determined whether this is a rare phenomenon. Theorizing discordance is not rare, we then analyzed data from cases with twin outcomes for congenital CMV infection, according to whether the twins were monozygotic or dizygotic, and calculated their corresponding concordance rates to estimate the broad-sense heritability. Lastly, we described other factors that might affect vertical transmission.ResultsFrom five articles following at-risk pregnancies, the rate of vertical transmission in twin pregnancies is 58.7% (95% CI 43.3-72.3%) whereas in singleton pregnancies it is 31.4% (95% CI: 29.0-34.0%)p= 0.0002. Of ten studies of larger cohorts of infants with congenital CMV infection, 21 of 42 twin pairs with at least one twin infected were discordant for congenital CMV (50.0%, 95% CI: 34.4–65.6%) indicating discordance of congenital CMV infection in twin pairs is not rare. Of 28 studies covering 37 twin pairs where at least one twin had congenital CMV, and zygosity was known, eleven of thirteen monozygotic twin pairs (84.6%; 95% CI: 53.7-97.3%) were concordant for CMV infection, and nine of twenty-four dizygotic twin pairs (37.5%; 95% CI: 19.6-59.2%) were concordant for infection giving an estimated hereditability of 94.2%. Within these 37 twin pairs, factors such as primary or recurrent maternal infection, prematurity, growth discordance, and sex are described; however, in many of these cases these factors are unknown.ConclusionThe rate of vertical transmission of congenital CMV is higher for twins than singletons. Discordance of congenital CMV in twins is not rare and suggests a possible genetic susceptibility to congenital CMV.
Strain Transformation Adjacent to the West Qinling Orogen: Implications for the Growth of the Northeastern Tibetan Plateau
Authors: Li Zhangjun , Cheng Feng , Hao Ming , Young Zachary M. , Song Shangwu , Yang Fan , Zhuang Wenquan 
Published: July 2021

The West Qinling orogen has played an important role in accommodating the deformation in the northeastern Tibetan Plateau induced by the India-Eurasia convergence. Here we construct a vertical land motion (VLM) model based on the latest leveling observations adjacent to the West Qinling orogen. Combined with the horizontal deformation field, the crustal deformation pattern in this area is investigated. Additionally, slip rate and coupling coefficients of the West Qinling fault, the longest fault separating the West Qinling orogen from the Lanzhou (Longxi) block, are inverted and constrained with GPS and VLM observations. Results show that the West Qinling fault slips slowly at a rate of 1–2 mm/yr and is strongly coupled with a moment magnitude deficit of Mw7.4. The crustal uplift rates adjacent to the West Qinling orogen are 0–3 mm/yr; which combined with 0–12.5 × 10−9/yr contraction rates, suggests that strain transformation plays a key role in controlling the tectonic uplift in the West Qinling orogen, and furthers our understanding of the contemporary geomorphic and topographic features. We identify a significant deformation transition belt at longitudes of 105°–106°E, which indicates that crustal deformation, induced from the northeastern expansion of the Tibetan Plateau, is mainly constrained to the plateau, rather than accommodated by crustal materials escaping eastward along the Qinling Mountains.
Identification of Candidate Blood mRNA Biomarkers in Intracerebral Hemorrhage Using Integrated Microarray and Weighted Gene Co-expression Network Analysis
Authors: Jin Feng , Li Lei , Hao Yuehan , Tang Ling , Wang Yuye , He Zhiyi 
Published: July 2021

PurposeIntracerebral hemorrhage (ICH) is a serious public health hazard due to its high morbidity, disability, and mortality. Currently, the exact molecular mechanisms of ICH are unknown. We tried to identify the ICH-related candidate blood messenger RNA (mRNA) biomarkers by microarray analysis and weighted gene co-expression network analysis (WGCNA).Materials and MethodsWe collected the blood samples from patients with ICH (n = 4) and from vascular risk factor (VRF) controls (n = 4) and analyzed the mRNA expression profiles by competitive endogenous RNA (ceRNA) microarray. Differentially expressed genes (DEGs) were identified and then a weighted gene co-expression network was constructed. Modules with clinical significance were distinguished. Then, we downloaded two Gene Expression Omnibus (GEO) datasets (GSE24265 and GSE125512). Candidate mRNAs were identified by taking the intersection of the DEGs in our microarray, the interesting genes in the key module, and the DEGs in GSE24265. Functional analysis involving Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and construction of a protein–protein interaction (PPI) network were conducted.ResultsA total of 340 DEGs in our microarray were identified between the ICH group and the control group. Among the eight gene modules established by WGCNA, the yellow module containing 191 genes was the most strongly associated with ICH. Four candidate mRNAs (C3AR1, PAWR, ARNTL2, and LDLRAD4) were identified. In the early stage of ICH (within 24 h), C3AR1, PAWR, and ARNTL2 were highly expressed in the perihematomal tissue, but with low expressions in peripheral blood; in the late stage (72 h after the first blood draw), an obvious upward trend of C3AR1 and PAWR in peripheral blood was seen. Functional analysis showed that candidate mRNAs were concerned with multiple pathways, such as the Wnt signaling pathway and calcium signaling pathway. They might affect the process of ICH through neuroinflammation, cell apoptosis, and pyroptosis.ConclusionWe identified four candidate blood mRNAs (C3AR1, PAWR, ARNTL2, and LDLRAD4) related to ICH. They showed different expression patterns in peripheral blood and perihematomal tissues and changed with time. They might play important roles in ICH through neuroinflammation, cell apoptosis, and pyroptosis and might shed new light to novel biomarkers or therapeutic targets in ICH.
Multi-Omics Profiling Identifies Risk Hypoxia-Related Signatures for Ovarian Cancer Prognosis
Authors: Chen Xingyu , Lan Hua , He Dong , Xu Runshi , Zhang Yao , Cheng Yaxin , Chen Haotian , Xiao Songshu , Cao Ke 
Published: July 2021

BackgroundOvarian cancer (OC) has the highest mortality rate among gynecologic malignancy. Hypoxia is a driver of the malignant progression in OC, which results in poor prognosis. We herein aimed to develop a validated model that was based on the hypoxia genes to systematically evaluate its prognosis in tumor immune microenvironment (TIM).ResultsWe identified 395 hypoxia-immune genes using weighted gene co-expression network analysis (WGCNA). We then established a nine hypoxia-related genes risk model using least absolute shrinkage and selection operator (LASSO) Cox regression, which efficiently distinguished high-risk patients from low-risk ones. We found that high-risk patients were significantly related to poor prognosis. The high-risk group showed unique immunosuppressive microenvironment, lower antigen presentation, and higher levels of inhibitory cytokines. There were also significant differences in somatic copy number alterations (SCNAs) and mutations between the high- and low-risk groups, indicating immune escape in the high-risk group. Tumor immune dysfunction and exclusion (TIDE) and SubMap algorithms showed that low-risk patients are significantly responsive to programmed cell death protein-1 (PD-1) inhibitors.ConclusionsIn this study, we highlighted the clinical significance of hypoxia in OC and established a hypoxia-related model for predicting prognosis and providing potential immunotherapy strategies.