bluuebunny/crossref_metadata_2025_split huggingface dataset:
Angiotensin II elicits robust calcium oscillations coordinated within juxtaglomerular cell clusters to suppress renin secretion
Authors: Yamaguchi Hiroki , Guagliardo Nick A. , Smith Jason P. , Xu Fang , Yamaguchi Manako , Almeida Lucas F , Matsuoka Daisuke , Medrano Silvia , Gomez R. Ariel , Sequeira-Lopez Maria Luisa S. 
Published: December 2024

AbstractBACKGROUNDJuxtaglomerular (JG) cells are sensors that control blood pressure and fluid-electrolyte homeostasis. In response to a decrease in perfusion pressure or changes in the composition and/or volume of the extracellular fluid, JG cells release renin, which initiates an enzymatic cascade that culminates in the production of angiotensin II (Ang II), a potent vasoconstrictor that restores blood pressure and fluid homeostasis. In turn, Ang II exerts a negative feedback on renin release, thus preventing excess circulating renin and the development of hypertension. How Ang II suppresses renin release from JG cells remains elusive. Ang II may inhibit renin release via increased systemic pressure sensed by JG cells, or through a direct effect on Ang II receptors in JG cells, which in turn mediate intracellular calcium (Ca2+) mobilization, a known suppressor of renin release. However, the intricate cellular events mediating Ca2+-induced renin inhibition by Ang II are not fully understood. Further, the unique structural organization of the juxtaglomerular apparatus (JGA), with JG cells clustered around afferent arterioles, suggests complex intercellular interactions, potentially facilitating coordinated Ca2+activity in response to Ang II. Here, we investigate the cellular processes that control Ca2+mobilization and the signaling mechanisms elicited when JG cells are stimulated with Ang II within the intact anatomical context of the JGA. By examining these processes, we aim to elucidate the role of cellular organization in Ca2+-mediated signaling and its impact on renin regulation within the JGA.OBJECTIVETo define intra- and inter-cellular Ca2+dynamics, identify the driving ion channels, and elucidate their functional role in Ang II-stimulated JG cells within the native kidney structure.METHODS AND RESULTSWe generated mice expressing JG cell-specific GCaMP6f, a genetically encoded Ca2+indicator, under theRen1cpromoter.Ex vivoCa2+imaging in acutely prepared kidney slices revealed that JG cells within clusters exhibit coordinated, robust Ca2+oscillations in response to Ang II stimulation, contrary to previous observations in isolated cells. These oscillations showed dose-dependent increases in occurrence and correlated with suppressed renin secretion. Pharmacological inhibition identified key drivers of these oscillations: endoplasmic reticulum Ca2+storage and release, extracellular Ca2+uptake via ORAI channels, and intercellular communication through gap junctions. Blocking ORAI channels and gap junctions alleviated Ang II inhibition of renin secretion.CONCLUSIONIn intact kidney slices, Ang II elicits synchronized Ca2+oscillations in JG cells, driven by endoplasmic reticulum-derived Ca2+release, ORAI channels, and gap junctions, leading to suppressed renin secretion.
Cytosolic peroxiredoxin Tsa1 influences acetic acid metabolism and pH homeostasis in wine yeasts
Authors: Garrigós Víctor , Picazo Cecilia , Dengler Lisa , Ewald Jennifer C. , Matallana Emilia , Aranda Agustín 
Published: December 2024

AbstractAcetic acid is a key metabolite in yeast fermentation, influencing wine quality through its role in volatile acidity. InSaccharomyces cerevisiae, acetic acid production involves aldehyde dehydrogenases, primarily Ald6 during fermentation and Ald4 under respiration. However, the regulatory mechanisms of these enzymes throughout fermentation and how they differ in commonly used strains remain partially unclear. This study explores the cytosolic peroxiredoxin Tsa1 as a novel regulator of acetic acid metabolism.TSA1gene deletion revealed strain-dependent effects on acetic acid metabolism and tolerance, showing reduced production and enhanced consumption in laboratory media. Under respiration, Ald4-driven acetic acid production, which raises extracellular pH, was mitigated by the absence of Tsa1. During wine fermentation,TSA1deletion decreased the initial acetic acid surge by downregulatingALD6transcription and enzymatic activity. These findings position Tsa1 as a metabolic regulator and a potential target for modulating acetic acid levels to manage volatile acidity and wine quality.
Modeling the emergent metabolic potential of soil microbiomes in Atacama landscapes
Authors: Andreani-Gerard Constanza M. , Jiménez Natalia E. , Palma Ricardo , Muller Coralie , Hamon-Giraud Pauline , Le Cunff Yann , Cambiazo Verónica , González Mauricio , Siegel Anne , Frioux Clémence , Maass Alejandro 
Published: December 2024

AbstractBackgroundSoil microbiomes harbor complex communities and exhibit important ecological roles resulting from biochemical transformations and microbial interactions. Difficulties in characterizing the mechanisms and consequences of such interactions together with the multidimensionality of niches hinder our understanding of these ecosystems. The Atacama Desert is an extreme environment that includes unique combinations of stressful abiotic factors affecting microbial life. In particular, the Talabre Lejía transect has been proposed as a unique natural laboratory for understanding adaptation mechanisms.ResultsWe propose a systems biology-based computational framework for the reconstruction and simulation of community-wide and genome-resolved metabolic models, in order to provide an overview of the metabolic potential as a proxy of how microbial communities are prepared to respond to the environment. Through a multifaceted approach that includes taxonomic and functional profiling of microbiomes, simulation of the metabolic potential, and multivariate analyses, we were able to identify key species and functions from six contrasting soil samples across the Talabre Lejía transect. We highlight the functional redundancy of whole metagenomes, which act as a gene reservoir from which site-specific functions emerge at the species level. We also link the physicochemistry from the puna and the lagoon samples to specific metabolic machineries that could be associated with their adaptation to the unique environmental conditions found there. We further provide an abstraction of community composition and structure for each site that allows to describe them as sensitive or resilient to environmental shifts through putative cooperation events.ConclusionOur results show that the study of community-wide and genome-resolved metabolic potential, together with targeted modeling, may help to elucidate the role of producible metabolites in the adaptation of microbial communities. Our framework was designed to handle non-model microorganisms, making it suitable for any (meta)genomic dataset that includes nucleotide sequence data and high-quality environmental metadata for different samples.
Confounder-aware foundation modeling for accurate phenotype profiling in cell imaging
Authors: Papanastasiou Giorgos , Sanchez Pedro P. , Christodoulidis Argyrios , Yang Guang , Pinaya Walter Hugo Lopez 
Published: December 2024

AbstractImage-based profiling is rapidly transforming drug discovery, offering unprecedented insights into cellular responses. However, experimental variability hinders accurate identification of mechanisms of action (MoA) and compound targets. Existing methods commonly fail to generalize to novel compounds, limiting their utility in exploring uncharted chemical space. To address this, we present a confounder-aware foundation model integrating a causal mechanism within a latent diffusion model, enabling the generation of balanced synthetic datasets for robust biological effect estimation. Trained on over 13 million Cell Painting images and 107 thousand compounds, our model learns robust cellular phenotype representations, mitigating confounder impact. We achieve state-of-the-art MoA and target prediction for both seen (0.66 and 0.65 ROC-AUC) and unseen compounds (0.65 and 0.73 ROC-AUC), significantly surpassing real and batch-corrected data. This innovative framework advances drug discovery by delivering robust biological effect estimations for novel compounds, potentially accelerating hit expansion. Our model establishes a scalable and adaptable foundation for cell imaging, holding the potential to become a cornerstone in data-driven drug discovery.
Foveal action for the control of extrafoveal vision
Authors: Zhang Tong , Tian Xiaoguang , Malevich Tatiana , Baumann Matthias P. , Hafed Ziad M. 
Published: December 2024

AbstractMicrosaccades have been convincingly linked to extrafoveal covert attention shifts for more than two decades. However, the direction of causality between individual microsaccade generation and an alteration in both extrafoveal visual sensitivity and behavior remains debated: do microsaccades merely reflect, perhaps probabilistically, an altered extrafoveal sensitivity, or is the act of generating microsaccades sufficient to, on its own, modify such sensitivity? Using a novel exploitation of real-time retinal image stabilization, behavior, and neurophysiology in the superior colliculus, we show that exclusive experimental control over foveal oculomotor state is entirely sufficient to influence extrafoveal sensitivity. This happens for eccentricities as large as ∼50 times those associated with microsaccades, and it also takes place in the absence of any differential attentional demands. Most importantly, such influence is mediated through well-known, classic pre- and post-saccadic visual processing changes. Thus, seemingly-innocuous subliminal eye movements do constitute an integral component of cognitive processes like attention.
Habitat-specificity in SAR11 is associated with a handful of genes under high selection
Authors: Tucker Sarah J. , Freel Kelle C. , Eren A. Murat , Rappé Michael S. 
Published: December 2024

AbstractThe orderPelagibacterales(SAR11) is the most abundant group of heterotrophic bacteria in the global surface ocean, where individual sublineages likely play distinct roles in oceanic biogeochemical cycles. Yet, understanding the determinants of niche partitioning within SAR11 has been a formidable challenge due to the high genetic diversity within individual SAR11 sublineages and the limited availability of high-quality genomes from both cultivation and metagenomic reconstruction. Here, we take advantage of 71 new SAR11 genomes from strains we isolated from the tropical Pacific Ocean to evaluate the distribution of metabolic traits across thePelagibacteraceae,a recently classified family within the orderPelagibacteralesencompassing subgroups Ia and Ib. Our analyses of metagenomes generated from stations where the strains were isolated reveals distinct habitat preferences across SAR11 genera for coastal or offshore environments, and subtle but systematic differences in metabolic potential that support these observations. We also observe higher levels of selective forces acting on habitat-specific metabolic genes linked to SAR11 fitness and polyphyletic distributions of habitat preferences and metabolic traits across SAR11 genera, suggesting that contrasting lifestyles have emerged across multiple lineages independently. Together, these insights reveal niche-partitioning within sympatric and parapatric populations of SAR11 and demonstrate that the immense genomic diversity of SAR11 bacteria naturally segregates into ecologically and genetically cohesive units, or ecotypes, that vary in spatial distributions in the tropical Pacific.
Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance
Authors: Saado Indira , Brabham Helen J. , Bennett Josh W. , Lam Anson Ho Ching , Hernández-Pinzón Inmaculada , Moscou Matthew J. , De la Concepcion Juan Carlos , Banfield Mark J. 
Published: December 2024

SummaryIntracellular immune receptors protect plants from microbial invasion by detecting and responding to pathogen-derived effector molecules, often triggering cell death responses. However, pathogen effectors can evolve to avoid immune recognition, resulting in devastating diseases that threaten global agriculture. Here, we show that an integrated Exo70 domain from the barley NLR RGH2 can interact with both the rice blast pathogen effector AVR-Pii and a closely related wheat blast variant. We used structure-led engineering to develop RGH2+that shows increased binding affinity towards AVR-Pii variants and increased cell death responses on heterologous expression inNicotiana benthamiana. Infection assays in transgenic barley lines harbouring RGH2+(along with the paired NLR RGH3) show reduced virulence towards blast strains expressing AVR-Pii variants. These results demonstrate the potential of engineering NLR receptors as an effective strategy for improving resistance towards one of the most destructive diseases affecting cereal production.
Optical signatures of strain-induced ferromagnetism in a
LaCoO3
thin film
Authors: Abadizaman F. , Munzar D. , Kiaba M. , Dubroka A. 
Published: December 2024

Using spectroscopic ellipsometry, we studied the optical conductivity of LaCoO3 with various degrees of strain. The optical response of the compressively strained LaCoO3 film is qualitatively similar to the one of the unstrained LaCoO3 polycrystalline sample and exhibits a redistribution of the spectral weight between ∼0.2 and 6 eV, which is most likely related to the thermal excitation of the high-spin (HS) states. The optical response of the ferromagnetic (FM) tensile strained film exhibits clear signatures of the FM state. Below the Curie temperature Tc=82 K, a spectral weight transfer sets on from high energies (between 3.3 and 5.6 eV) to low energies (between 0.2 and 3.3 eV). The temperature dependence of the low-energy spectral weight can be understood in the framework of the HS biexciton model of Sotnikov [] as corresponding to the increase of the concentration of the HS states that are stabilized below Tc. The magnitude of the redistribution of the spectral weight due to the formation of the FM state is sizable and corresponds to 0.009 e per Co ion. We discuss it in terms of the effective kinetic energy of Co 3d bands.

Published by the American Physical Society
2024
Research on the electrochemical dissolution behavior of Ni-based superalloys after different oxidation treatments in NaCl solution
Authors: Jiang Hairui , Guan Jianjun , Zhao Yan , Qu Jinglong , Yang Yanhong 
Published: December 2024

Purpose
This study aims to investigate the corrosion resistance and electrochemical dissolution behavior of superalloys treated by different oxidation treatments.

Design/methodology/approach
Ni-based superalloys were subjected to oxidation treatment at 1000 °C for 10 h, 1150 °C for 10 h and 1200 °C for 20 h. The microstructure, electrochemical dissolution behavior, elemental distribution, as well as compactness and composition of the oxide layer, were studied.

Findings
The results show that both the thickness and the granular oxide size of the oxide layer on Ni-based superalloys increase with longer oxidation times and higher temperatures. The electrochemical dissolution efficiency of Ni-based superalloys decreases with increasing oxidation time and temperature. The reduced electrochemical dissolution efficiency observed in Ni-based superalloys oxidation-treated at 1200 °C for 20 h is primarily attributed to the thicker oxide layer, which contains the highest Cr oxide content.

Originality/value
The findings contribute to the advancement of recycling and utilization of Ni-based superalloy scrap.
Breaking down to build up: how deconstruction and carbon finance foster sustainable, resilient construction in the industry 5.0 era
Authors: Murali Drisya , Suresh M. , Raman Raghu 
Published: December 2024

Purpose
This study aims to seek and identify key enablers influencing the integration of deconstruction and carbon finance to improve sustainable and resilient construction in the Industry 5.0 era and then examines how these enablers relate to one another and rank, classify and prioritize them appropriately.

Design/methodology/approach
This study adopts the Integrated Decision-Making Trial and Evaluation Laboratory-Interpretive Structural Modeling-Matrice d’impacts Croises Multiplication Appliquee a un Classment (DEMATEL-ISM-MICMAC) methodology.

Findings
The key enablers are the circular economy approach with closed-loop material flow, prioritizing material recovery and reuse, and structuring carbon credits for affordability.

Research limitations/implications
The construction industry can advance toward environmentally friendly, sustainable building techniques and help achieve the larger objectives of carbon neutrality and environmental preservation by adopting the study’s guiding principles.

Practical implications
This study implies switching to circular rather than linear economic methods. It is still unrealistic to achieve zero carbon emissions, which is why carbon finance is important because it allows companies to trade carbon credits and encourages negative carbon activities and to structure and trade carbon credits affordably so that businesses of all sizes can participate. To extend the lives of current materials and reduce waste, it is imperative to maximize their reuse and minimize their disposal.

Originality/value
The novelty of this research lies in introducing carbon trade finance concepts to the construction sector, particularly focusing on the deconstruction phase, to enhance sustainability and resilience in construction practices in the Industry 5.0 era.
Personal leadership resources used by principals during a period of contingency
Authors: del Arco Isabel , Ramos-Pla Anabel , Tintoré Mireia , Cabral Ilídia 
Published: December 2024

PurposeThe objective of the present study is to compare the Personal Leadership Resources (PLR) of primary school principals in Spain and Portugal, before and during the COVID-19 pandemic.Design/methodology/approachA quantitative methodology was used to analyze the data obtained from a questionnaire that measured the PLR of a sample of 308 principals from both countries (Portugal and Spain).FindingsThe results showed that the education leaders utilized their PLR with less intensity in a health emergency. Also, it was shown that the PLR related to the need to know effective practices, the efficiency in problem-solving, and the management of one’s emotions were crucial in moments of crisis. It is essential to identify the PLR utilized in moments of crisis to influence the training of education leaders to manage future emergencies efficiently.Originality/valueThis is the first study comparing the PLR developed by school principals in Catalonia (Spain) and Portugal before and during the pandemic.
Exploring the role of ethical leadership and personality traits in shaping ethical behavior among Ugandan security officers
Authors: Kyambade Mahadih , Namatovu Afulah 
Published: December 2024

PurposeThe study investigates the mediating role of ethical leadership on the association between personality traits and ethical behavior among police officers in Uganda. The primary aim is to understand how personality traits influence ethical behavior and the role ethical leadership plays in this dynamic.Design/methodology/approachEmploying a quantitative, descriptive research design, data were collected through convenience sampling from 259 police officers across various stations. A self-administered questionnaire comprising closed-ended questions was utilized to assess the officers’ personality traits, ethical behavior and ethical leadership, with responses measured on a five-point Likert scale. The data were analyzed using structural equation modeling (SEM) with Smart PLS software, incorporating bootstrapping techniques to evaluate direct and indirect effects among the constructs.FindingsThe findings indicate a positive association between personality traits and ethical behavior, with ethical leadership significantly mediating this relationship.Practical implicationsSpecifically, officers with favorable personality traits are more likely to engage in ethical behavior, facilitated by the presence of ethical leadership. These results emphasize the necessity of promoting ethical leadership to enhance the ethical conduct of police officers in Uganda.Originality/valueThis research contributes original insights into the interplay between personality traits, ethical leadership and ethical behavior within the Ugandan police context. The practical implications suggest that law enforcement agencies should prioritize ethical leadership development initiatives, as these can significantly enhance ethical standards among officers, thereby improving the overall integrity of the police force and fostering public trust.
Intellectual capital management as a catalyst for organizational agility and performance in post-COVID-19 Romanian SMEs
Authors: Stratone Madalina-Elena , Vatamanescu Elena-Madalina 
Published: December 2024

PurposeThe study seeks to investigate the impact of intellectual capital (IC) management on the performance and agility of small- and medium-sized enterprises (SMEs) in Romania, with a particular focus on the post-COVID-19 recovery period. By examining the roles of human, structural and relational capital, the study sets out to provide insights into how SMEs can build resilience against future socioeconomic disruptions and achieve a sustainable competitive advantage.Design/methodology/approachA quantitative research method was employed, utilizing a questionnaire distributed to 121 managers from Romanian SMEs. The collected data was analyzed using SmartPLS 4 software to test hypotheses regarding the role of intellectual capital management in enhancing organizational agility and performance.FindingsIC, especially human capital, substantially influences organizational agility and performance in SMEs. Findings confirm that adapting to a dynamic work environment requires that SMEs invest in employee development, foster strong internal and external relationships, and embrace new technologies. Furthermore, building an adaptive organizational culture open to change and learning is essential for sustaining long-term competitive advantage in an unpredictable market.Research limitations/implicationsThe study advances theoretical and practical recommendations for academics, managers and policymakers on leveraging IC to enhance organizational agility and performance, offering a roadmap for organizations navigating systemic crises. The main limitation is the context-driven focus of the research on Romanian SMEs.Practical implicationsSwitching to the practical implications, this research offers managers of small and medium-sized businesses (SMEs) in Romania insightful knowledge about how to strategically manage IC to improve organizational agility and performance and illustrates the need for the SMEs to invest in the human capital, to adapt quickly to the market changes and to invest their resources in integrating new technologies, alongside with building strong relationships (both inside and outside the organization) and to cultivate an adaptive organizational culture that is open to change and to learn.Social implicationsThis study provides guidance to the Romanian managers in finding the key to success, by ensuring competitiveness in the nowadays dynamic market, foster innovation and boost the agility and the performance of the organization.Originality/valueThe research is the first one to address the strategic importance of IC management in Romanian SMEs with a view to attaining agility and performance in turbulent times.