bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress
Authors: Griffin Wezley C. , McKinzey David R. , Klinzing Kathleen N. , Baratam Rithvik , Trakselis Michael A. 
Published: December 2021

AbstractThe minichromosome maintenance (MCM) 8/9 helicase is a AAA+ complex involved in DNA replication-associated repair. Despite high sequence homology to the MCM2-7 helicase, an active role for MCM8/9 has remained elusive. We interrogated fork progression in cells lacking MCM8 or 9 and find there is a functional partitioning. Loss of MCM8 or 9 slows overall replication speed and increases markers of genomic damage and fork instability, further compounded upon treatment with hydroxyurea. MCM8/9 acts upstream and antagonizes the recruitment of BRCA1 in nontreated conditions. However, upon treatment with fork stalling agents, MCM9 recruits Rad51 to protect and remodel persistently stalled forks. The helicase function of MCM8/9 aids in normal replication fork progression, but upon excessive stalling, MCM8/9 directs additional stabilizers to protect forks from degradation. This evidence defines novel multifunctional roles for MCM8/9 in promoting normal replication fork progression and promoting genome integrity following stress.
Structural basis for substrate recognition, ligation and activation by a hyperactive Asn peptide ligase from Viola yedoensis
Authors: Hu Side , Sahili Abbas El , Kishore Srujana , Wong Yee Hwa , Hemu Xinya , Goh Boon Chong , Wang Zhen , Tam James P. , Liu Chuan-Fa , Lescar Julien 
Published: December 2021

ABSTRACTPeptide asparaginyl ligases (PALs) belong to a limited class of enzymes from cyclotide-producing plants, that perform site-specific ligation reactions after a target peptide Asx (Asn/Asp) binds to the ligase active site. How PALs specifically recognize their polypeptide substrates has remained elusive especially at the prime binding side of the enzyme. Here we captured VyPAL2, a catalytically efficient PAL from Viola yedoensis, in an activated state, with and without a bound substrate. The bound structure shows one ligase with the N-terminal polypeptide tail from another ligase molecule trapped at its active site, revealing how Asx inserts in the enzyme’s S1 pocket and why a hydrophobic residue is required at the substrate P2’ position. Beside illustrating the role played by P1 and P2’ residues as primary anchors for the enzyme reaction, these results provide a mechanistic explanation for the role of the “Gatekeeper” residue at the surface of the S2 pocket, in shifting the non-prime portion of the substrate and, as a result, the activity towards either ligation or hydrolysis. These results detail the molecular events that occur during proenzyme maturation in the plant vacuolar compartment, suggest a mechanism for ligation, and will inform the design of peptide ligases with tailored specificities.One sentence summaryWe captured VyPAL2, a catalytically efficient plant peptide ligase with a bound substrate, providing the molecular basis for substrate recognition and ligation.
Effects of employee identity salience on content sharing in social networks
Authors: Zhang Xueting , Tang Tanya (Ya) , Chen Man , Wang Feng 
Published: December 2021

PurposeThis research explores whether, when and why employee identity salience influences content sharing of employee's friends in social networks and further investigates two moderating conditions of network overlap and deal content.Design/methodology/approach In Study 1, the authors analyzed a field data related to 20,715 users on the largest social network platform in China. In Studies 2 and 3, the authors verified the findings of Study 1 and tested the underlying mechanism with two experiments.Findings The results showed that employee identity salience could increase sharing likelihood of content receiver, especially when the employee had higher network overlap with receiver. However, when the content contained deal-related information, the receiver was less likely to share this content from employee with salient identity. The authors also found that perceived information credibility acted as a mediator in above relationships.Originality/value This research is the first to test the effects of employee identity salience on content sharing by considering both content type and network characteristics. The authors also provide insights into the mediating role of information credibility, which enriches the content sharing and social network literature.
Situated rationalities and management control change – an empirical note on key actors, situated rationalities and generalised practices
Authors: Bertz Julie , Quinn Martin 
Published: December 2021

Purpose
This paper aims to offer an incremental contribution, augmenting the notion of situated rationality as proposed by terBogt and Scapens (2019). Through insights from empirical data, the authors explore the role of situated rationalities of key individual actors in processes of management control change.

Design/methodology/approach
A qualitative research approach was adopted with qualitative data collected in a single public service organisation through face-to-face interviews, organisation documentation and observations.

Findings
The findings present the important role of key individual actors in bringing about a new situated rationality in a housing department. External austerity forces combined with actors’ experience rationalities acted as a stimulus to change existing management control practices in the management of public services.

Originality/value
The paper conceptualises “experience” rationality, capturing the experiences of a key actor, including elements of leadership style. Drawing on a story of a complex process of management control change, this paper thus reveals interactions between generalised practices and situated rationalities which were not highlighted by the extended framework of terBogt and Scapens.
The independent and joint contribution of objective and subjective socioeconomic status on oral health indicators
Authors: Schuch Helena S. , Peres Karen G. , Haag Dandara G. , Boing Antonio F. , Peres Marco A. 
Published: December 2021

AbstractObjectivesThe understanding of how subjective socioeconomic status (SSS) relates to objective socioeconomic status (OSS), and how both conditions act together in oral health outcomes is still unclear. This study aims to test the independent and joint association between OSS and SSS with oral health, to assess the role of socioeconomic status discrepancies, and to evaluate the role of SSS in the association between OSS and oral health.MethodsData from 1140 adults from a population‐based study in Southern Brazil were used. We applied diagonal reference models DRM to disentangle the effects of OSS (education) and SSS (MacArthur Scale) to oral health outcomes. The outcomes were functional dentition clinically evaluated (FD ≥20 teeth) and self‐reported oral health SROH. We also examined the discrepancy between OSS and SSS to oral health indicators and the effect measure modification (EMM) of SSS on the association between OSS and oral health.ResultsSubjective socioeconomic status and OSS contributed equally to SROH, while OSS explained a substantially higher amount of FD than SSS (0.85 vs 0.15). An EMM of SSS was found on the association between OSS and fair/poor SROH, with a relative excess risk due to interaction (RERI) of 1.08. Less evidence of EMM was found for FD (RERI = 0.14). Individuals with lower SSS and OSS had four times the risk of the outcomes than the reference group.ConclusionsAdults with concordant lower SSS and OSS have a worse oral health than those with concordant higher status. There was evidence that the association between OSS and SROH is modified by SSS.
The stability of social categories
Authors: Roth Abraham Sesshu 
Published: December 2021

AbstractOne important thesis Ásta defends in Categories We Live By is that social properties and categories are somehow dependent on our thoughts, attitudes, or practices—that they are inventions of the mind, projected onto the world. Another important aspect of her view is that the social properties are related to certain base properties; an individual is placed in a category when the relevant base properties are thought to hold of them. I see the relationship between the social and the base as connected to the problem of explaining how the social properties are sufficiently stable so as to be taken seriously, both in theoretical endeavors as well as in practical matters of how we relate to each other. In this light, I identify stability constraints for an adequate account of social categories. I argue that certain distinctive aspects of Ásta's conferralist view of social categories, such as the radical contextualism in her account of gender, undermine the stability of categories and are at odds with taking social categories seriously. I end with the suggestion that a distinctive “sheltered” form of normativity might help us do justice to Ásta's insights while avoiding some of the destabilizing elements of conferralism.
Number of growth days and not length of the growth period determines radial stem growth of temperate trees
Authors: Etzold Sophia , Sterck Frank , Bose Arun K. , Braun Sabine , Buchmann Nina , Eugster Werner , Gessler Arthur , Kahmen Ansgar , Peters Richard L. , Vitasse Yann , Walthert Lorenz , Ziemińska Kasia , Zweifel Roman 
Published: December 2021

AbstractRadial stem growth dynamics at seasonal resolution are essential to understand how forests respond to climate change. We studied daily radial growth of 160 individuals of seven temperate tree species at 47 sites across Switzerland over 8 years. Growth of all species peaked in the early part of the growth season and commenced shortly before the summer solstice, but with species‐specific seasonal patterns. Day length set a window of opportunity for radial growth. Within this window, the probability of daily growth was constrained particularly by air and soil moisture, resulting in intermittent growth to occur only on 29 to 77 days (30% to 80%) within the growth period. The number of days with growth largely determined annual growth, whereas the growth period length contributed less. We call for accounting for these non‐linear intra‐annual and species‐specific growth dynamics in tree and forest models to reduce uncertainties in predictions under climate change.
Adherence to discharge protocols and prostate‐specific antigen surveillance plans in men without a prostate cancer diagnosis
Authors: McAllister Bría Joyce 
Published: December 2021

AbstractProstate‐specific antigen (PSA) surveillance testing is a valuable tool in the ongoing care of men who have undergone investigations into potential prostate cancer, with a benign outcome. A literature review was conducted to assess the published evidence into discharge protocols for men who have undergone investigations into prostate cancer with benign outcomes, and compliance with, and attrition from, these protocols in this patient group. When insufficient literature was retrieved, the search was broadened to include men with low‐grade prostate cancer on active surveillance pathways. There is little evidence in the published literature surrounding the issue of compliance with discharge plans in the target group. Concordance with active surveillance plans is better documented, but evidence remains relatively scarce. Evidence from research into men with low‐grade prostate cancer shows that compliance with discharge plans is a complex issue with many factors affecting outcomes. The attitudes of physicians, along with those of patients, are a major component, as is the availability of consistent and accessible guidelines. The literature surrounding men who have had benign outcomes from investigations into potential prostate cancer tends to make little reference to the issues surrounding concordance, and it is clear that this is not a well‐researched area. Concerns were raised surrounding the finding that up to 50% of men who have not adhered to their PSA surveillance plans have had other routine bloods drawn by their general practitioners (GPs) raising the issue of GP non‐compliance. The issue of compliance with PSA surveillance plans in men without a prostate cancer diagnosis is clearly a complex one which is compounded by the lack of nationally standardized discharge protocols. Further research is required to design a standardized discharge protocol to improve compliance with surveillance pathways, thereby improving early detection of developing cancers.
Expression of the miR‐17~92a cluster of microRNAs by regulatory T cells controls blood glucose homeostasis
Authors: Zhang Yangnan , Skinner Jarrod P , Chong Mark MW 
Published: December 2021

AbstractRegulatory T cells (Tregs) are a specialized immune cell type that play important roles in regulating immune responses. However, those found in adipose tissue, particularly visceral adipose tissue (VAT), have also been shown to exert metabolic regulatory functions. This study investigated the requirement of the miR‐17~92a cluster of microRNAs in VAT Tregs and the impact on blood glucose. This cluster of microRNAs is one that we previously showed to be important for the fitness of Tregs found in secondary lymphoid organs. It was found that male mice with Treg‐specific miR‐17~92a deficiency are resistant to impaired glucose tolerance induced by a high‐fat diet. However, high‐fat feeding still impaired glucose tolerance in female mice with Treg‐specific miR‐17~92a deficiency. There was an increase in KLRG1− naïve Tregs and a loss of KLRG1+ terminally differentiated Tregs in the VAT of Treg‐specific miR‐17~92a‐deficient male mice but not in female mice. The protection of male mice from high‐fat feeding was also associated with increased interleukin‐10 and reduced interferonγ expression by conventional CD4+ T cells and reduced interleukin‐2 expression by both CD4+ and CD8+ T cells in the VAT. Together this suggests that expression of miR‐17~92a by VAT Tregs regulates the effector phenotype of conventional T cells and in turn the metabolic function of adipose tissue and blood glucose homeostasis.
Validity of the strategic management simulations to predict real‐world productivity
Authors: MacNaughton Piers , Cockerill Anthony 
Published: December 2021

AbstractThe performance of individual businesses and the economy as a whole hinges on the productivity of human capital, yet measuring productivity at an individual level has proved difficult. The Strategic Management Simulation (SMS) methodology comprises several decision‐making assessment tools that have been used for several decades to estimate real‐world productivity. Performance on productivity measures using a shorter version of the SMS methodology was gathered from a sample of 111 knowledge workers from major corporations in the U.S. and compared with their income level. In linear models, every percentile increase in SMS performance was associated with a $791–$1,113 increase in income in each of the four domains of decision‐making performance provided by the SMS tool. The models had R2’s ranging from 0.65 to 0.75, indicating high levels of correlation between SMS performance and income levels. This study is the first to show linear relationships between SMS performance and other indicators of productivity, indicating that the SMS is a valid predictor of productivity and that SMS scores can be used to predict job satisfaction and performance.
Limits of Law in the Multilevel System: Explaining the European Commission's Toleration of Noncompliance Concerning Pharmaceutical Parallel Trade*
Authors: Zhang Yaning 
Published: December 2021

AbstractThis article aims to explain the under‐researched phenomenon of why the European Commission (the Commission), as ‘guardian of the Treaties’, tolerates member states’ noncompliance with the EU law. While major accounts of selective enforcement depict the Commission as a self‐serving political entrepreneur, this paper assumes that it is a trustee guardian of EU treaties that aims to safeguard the stability and integrity of the EU legal order. For this purpose, the Commission is theorized to strategically utilize toleration of noncompliance to evade jurisdiction overlap and norm collision. Relying on the detailed tracing of the Commission's enforcement leniency towards Slovakia regarding pharmaceutical parallel trade, this illustrative case study indicates that toleration of noncompliance is a necessary evil for the Commission and other stakeholders to navigate through a legal and political impasse. And it simultaneously preserves the delicate integrity of the existing legal order of EU free movement law.
Postoperative delirium screening tools for post‐anaesthetic adult patients in non‐intensive care units: A systematic review and meta‐analysis
Authors: Kim Sujeong , Choi Eunju , Jung Youngsun , Jang Insil 
Published: December 2021

AbstractAims and ObjectivesTo identify the most accurate postoperative delirium screening tools for detecting postoperative delirium among patients who underwent general anaesthesia surgery in general wards.BackgroundThe lack of detection of postoperative delirium can negatively affect the patient's condition, along with their postoperative treatment and rehabilitation, and it can prolong their hospitalisation, persists cognitive dysfunction and increases mortality. Screening for postoperative delirium in hospitalised patients as nursing assessment is routine clinical practice for early detection.DesignA systematic review and meta‐analysis.MethodsMEDLINE, Embase, CINAHL, KoreaMed and Cochrane electronic databases were searched using the key words delirium, postoperative, assessment or screening, and adult for articles published up to April 2020, with no limit on the year of publishing. Only prospective cohort studies reporting sensitivity and specificity values were included. We followed the recommendations of the Cochrane Handbook of Diagnostic Test Accuracy Reviews and the PRISMA checklist. The Quality Assessment of the Diagnostic Accuracy Studies‐2 tool was used for data extraction and quality assessment, while a bivariate random‐effects meta‐analysis model was used for pooling and comparing diagnostic accuracy and providing a summary of evidence.ResultsSix delirium assessment tools were evaluated from nine papers including 3088 patients. Due to the limited number of papers, the meta‐analysis included the Confusion Assessment Method (CAM) and its variants, Delirium Detection Score (DDS) and Nurses' Delirium Screening Checklist (NuDESC). Overall, NuDESC demonstrated higher sensitivity than CAM or DDS, while all showed high specificity (0.90 or greater).ConclusionThis review suggested that NuDESC can be employed as an accurate screening tool with high specificity for assessing postoperative delirium during routine checkups. However, it is necessary to consider suitable cut‐off values, which is the reference point, in accordance with the clinical setting and the patients’ condition.Relevance to clinical practiceNuDESC reported the best evidence of diagnostic accuracy, and we recommend clinical nurses to employ this easy‐to‐use and validated tool for daily screening of postoperative delirium in general wards to facilitate its early detection and the accurate estimation of its prevalence.
Religion‐State Interaction at the Local Level: Key Findings from a Survey of Religion and Local Elected Officials
Authors: Adler Gary J. , Mayrl Damon , Coley Jonathan S. , Sager Rebecca , Plutzer Eric 
Published: December 2021

AbstractRelationships between religion and state are a core focus for social scientists, but little is known about a central set of actors in “church‐state” relations in the United States: local elected officials (mayors, town councilpersons, city commissioners). We report on a unique, representative survey of local elected officials, examining their religiosity, their interactions with religion through governance (prayers, meetings, symbol placement requests), and their preferences for religion‐state relations. Our results show that local elected officials are no more religious than the general adult public, that they interact with religion in their formal governance at low rates, and that a quarter strongly prefer increased state engagement with religion. Minority religious affiliation, Democratic political affiliation, and urban context predict opposition to religion‐state engagement. We describe how local elected officials may produce local regimes of religion‐state interaction that vary by geographic location and suggest pathways for future research.
Multiplexed CRISPR/Cas9 editing of the long‐chain acyl‐CoA synthetase family in the diatom Phaeodactylum tricornutum reveals that mitochondrial ptACSL3 is involved in the synthesis of storage lipids
Authors: Hao Xiahui , Chen Wenchao , Amato Alberto , Jouhet Juliette , Maréchal Eric , Moog Daniel , Hu Hanhua , Jin Hu , You Lingjie , Huang Fenghong , Moosburner Mark , Allen Andrew E. , Gong Yangmin 
Published: December 2021

Summary

Long‐chain acyl‐CoA synthetases (LACS) play diverse and fundamentally important roles in lipid metabolism. While their functions have been well established in bacteria, yeast and plants, the mechanisms by which LACS isozymes regulate lipid metabolism in unicellular oil‐producing microalgae, including the diatom Phaeodactylum tricornutum, remain largely unknown.

In P. tricornutum, a family of five genes (ptACSL1–ptACSL5) encodes LACS activities. We generated single lacs knockout/knockdown mutants using multiplexed CRISPR/Cas9 method, and determined their substrate specificities towards different fatty acids (FAs) and subcellular localisations.

ptACSL3 is localised in the mitochondria and its disruption led to compromised growth and reduced triacylglycerol (TAG) content when cells were bubbled with air. The ptACSL3 mutants showed altered FA profiles in two galactoglycerolipids and phosphatidylcholine (PC) with significantly reduced distribution of 16:0 and 16:1. ptACSL5 is localised in the peroxisome and its knockdown resulted in reduced growth rate and altered molecular species of PC and TAG, indicating a role in controlling the composition of acyl‐CoAs for lipid synthesis.

Our work demonstrates the potential of generating gene knockout mutants with the mutation of large fragment deletion using multiplexed CRISPR/Cas9 and provides insight into the functions of LACS isozymes in lipid metabolism in the oleaginous microalgae.
Interrogating the Lead-Up to a Critical Speed in Rotordynamics
Authors: Virgin Lawrence N. 
Published: December 2021

AbstractThis paper presents a new approach to predicting an incipient critical speed in a rotating shaft. Based on the classical governing equations of motion for an eccentric mass on a flexible shaft (the Jeffcott rotor model), the approach is centered on examining the behavior of small perturbations or random disturbances to infer the approach of a critical speed (resonance). Such disturbances, that may be based on intentional probing, or simply the result of naturally occurring fluctuations, cause small transients. It is the changing nature of these transients (as characterized by their associated eigenvalues) that is used to assess the proximity to a critical speed. In this paper, the material developed is based on analysis, but generating the data from simulations or experiments will be the next step. The approach is a kind of stress-test, conceptually not dissimilar to structural health monitoring and damage detection but here directed toward the lead-up to resonance.
Type Specimens of the Small-Headed Flies (Diptera, Acroceridae) in the Collection of the Zoological Institute of the Russian Academy of Sciences, St. Petersburg
Authors: Nartshuk E. P. , Paramonov N. M. , Suleymanova T. A. 
Published: December 2021

Abstract
A catalogue of the types (holotypes, paratypes, and a lectotype) of the species of the family Acroceridae described by L.F. Hildebrandt, F.D. Pleske, and E.P. Nartshuk, deposited in the collection of the Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia, is presented. Photographs of the specimens and labels of the holotypes and lectotype are given.