bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequent Aneuploidy in Primary Human T Cells Following CRISPR-Cas9 cleavage
Authors: Nahmad A.D. , Reuveni E. , Goldschmidt E. , Tenne T. , Liberman M. , Horovitz-Fried M. , Khosravi R. , Kobo H. , Reinstein E. , Madi A. , Ben-David U. , Barzel A. 
Published: August 2021

SUMMARYMultiple ongoing clinical trials use site-specific nucleases to disrupt T cell receptor (TCR) genes in order to allow for allogeneic T cell therapy1–5. In particular, the first U.S. clinical trial using CRISPR-Cas9 entailed the targeted disruption of the TCR chains and programmed cell death protein 1 (PDCD1) in T cells of refractory cancer patients6. Here, we used the same guide RNA sequences and applied single-cell RNA sequencing (scRNAseq) to more than 7000 primary human T cells, transfected with CRISPR-Cas9. Four days post-transfection, we found a loss of chromosome 14, harboring the TCRα locus, in up to 9% of the cells, and a chromosome 14 gain in up to 1.4% of the cells. We further identified truncations of chromosome 7, harboring the TCRβ locus, in 9.9% of the cells. Loss of heterozygosity (LOH) was further validated using fluorescencein situhybridization (FISH) and the temporal dynamics of cleavage and incomplete repair were monitored using digital droplet PCR (ddPCR). Aneuploidy was found among all T cell subsets and was associated with transcriptional signatures of reduced proliferation and metabolism as well as with induced p53 activation and cell death. We conclude that aneuploidy and chromosomal truncations are frequent outcomes of CRISPR-Cas9 cleavage in clinical protocols. Monitoring and minimizing these aberrant products is crucial for future applications of genome editing in T cell engineering and beyond.
Two crystallographic forms and the absolute structure of 5α,14α-androstane
Authors: Crittenden Christopher M. , DiPasquale Antonio G. 
Published: August 2021

5α,14α-Androstane (C19H32) crystallizes in two different polymorphic forms in the same vapor diffusion experiment. The major form (Form I) crystallizes as thin plates in the space group P21, with Z = 4. These plates are twinned along a long c axis of length 43 Å and readily suffer from radiation damage when diffracted. The minor form (Form II) crystallizes as fine needles in the space group P212121, Z = 3. In the minor form, 5α,14α-androstane cocrystallizes with 5α,14α-androstan-17-one, an oxidation product of 5α,14α-androstane. The presence of 5α,14α-androstan-17-one in the minor form of the crystals was confirmed by HR-MS. Form II can be crystallized as a pure form without the ketone impurity using a different solvent system. High level density functional theory (DFT) lattice free energy calculations were performed and show that both pure forms are isoergic within the estimated error of the calculations.
Trends and characteristics of China's medical informatization policy from 1996 to 2020: a bibliometric analysis
Authors: Xu Man , Gan Dan , Pan Ting , Sun Xiaohan 
Published: August 2021

PurposeQualitative methods are not suitable to process high volumes of policy texts for exploring policy evolution. Therefore, it is hard to use qualitative methods to systematically analyze the characteristics of complex policy networks. So the authors propose a bibliometric research study for exploring policy evolution from time–agency–theme perspectives to excavate the rules and existing problems of China's medical informatization policy and to provide suggestions for formulating and improving the future medical informatization policies.Design/methodology/approachInitially, 615 valid samples are obtained by retrieving related China's medical informatization policy documents, and the joint policy-making agency network and the co-occurrence network models of medical informatization policies are defined, and then the authors research China's medical informatization policies from single-dimension and multi-dimension view.FindingsThe analysis results reveal that China's medical informatization policy process can be divided into four stages; the policy-making agencies are divided into four subgroups by community detection analysis according to the fast unfolding algorithm; the core policy theme keywords are identified based on the eigenvector centrality of the nodes in those networks; the focuses of theme terms are varied in different stages and the correlations between agencies and themes are gradually decentralized.Practical implicationsThese findings provide experience and evidence on leveraging informatics in the medical and healthcare field of China. Also, they can help scholars and practitioners better understand the current status and future directions of medical and healthcare informatics development in China and provide a reference to formulate and improve China's future medical informatization policies.Originality/valueThis study proposes a quantitative bibliometric-based research framework to describe transitions and trends of China's medical informatization policy.
Meta-frontier and measures of efficiency emphasising optimal corporate governance risk across countries
Authors: ElKelish Walaa Wahid , Zervopoulos Panagiotis 
Published: August 2021

PurposeThis paper aims to investigate the internal and external determinants of firms’ efficiency and develop optimal corporate governance risk benchmarks for the manufacturing sector across different countries.Design/methodology/approachCorporate governance risk data were acquired from Institutional Shareholder Services Europe SA. Data on firms’ efficiency and for explanatory and control variables were taken from the DataStream database. The generalised directional distance function data envelopment analysis (GDDF-DEA) model and its stochastic extension provided corporate efficiency measures and optimal corporate governance benchmarks. The authors used ordinary least squares multiple regression analysis with wild bootstrapping to test the study hypotheses.FindingsThe authors found significant differences between firms’ optimal and actual efficiency input/output variables and corporate governance risks in the manufacturing sector across countries. Internal firm characteristics such as group affiliations, product market competition and insider ownership and external institutional factors such as the legal system, the rule of law, control of corruption, law enforcement and cultural values are vital determinants of firms’ efficiency.Practical implicationsThis paper provides valuable guidance to enable corporate managers, regulators and policymakers to enhance firms’ efficiency and corporate governance practices.Originality/valueThis paper develops optimal corporate governance risk benchmarks and identifies the most critical internal and external factors affecting firms’ efficiency in the manufacturing sector in various countries. It also used a novel GDDF-DEA model, with the multi-parametric model for bias correction of efficiency estimator.
The internet of things in upscale hotels: its impact on guests’ sensory experiences and behavior
Authors: Pelet Jean-Éric , Lick Erhard , Taieb Basma 
Published: August 2021

Purpose
This study bridges the gap between sensory marketing and the use of the internet of things (IoT) in upscale hotels. This paper aims to investigate how stimulating guests’ senses through IoT devices influenced their emotions, affective experiences, eudaimonism (well-being), and ultimately, guest behavior. The authors examined the potential moderating effects of gender.

Design/methodology/approach
Research conducted comprised an exploratory study, which consisted of interviews with hotel managers (Study 1) and an online confirmatory survey (n = 357) among hotel guests (Study 2).

Findings
The results showed that while the senses of smell, hearing and sight had an impact on guests’ emotions, the senses of touch, hearing and sight impacted guests’ affective experiences. The senses of smell and taste influenced guests’ eudaimonism. The sense of smell had a greater effect on eudaimonism and behavioral intentions among women compared to men.

Research limitations/implications
This study concentrated on upscale hotels located in Europe. Further research may explore the generalizability of the findings (e.g. in other cultures, comparison between high-end and low-end hotels).

Practical implications
Managers of upscale hotels should apply congruent sensory stimuli from all five senses. Stimuli may be customized (“SoCoIoT” marketing). IoT in hotels may be useful in the aftermath of the COVID pandemic, as voice commands help guests avoid touching surfaces.

Originality/value
IoT can be applied in creating customized multi-sensory hotel experiences. For example, hotels may offer unique and diverse ambiances in their rooms and suites to improve guest experiences.
Nonlinear modelling and finite element analysis on the load-bearing capacity of RC beams with considering the bond–slip effect
Authors: Pandimani Pandimani , Ponnada Markandeya Raju , Geddada Yesuratnam 
Published: August 2021

Purpose
This paper aims to present nonlinear numerical simulations using the versatile finite element (FE) analysis tool ANSYS and theoretical analysis based on code provisions to assess the load-carrying capacity of reinforced concrete (RC) beams under two-point monotonic static loadings.

Design/methodology/approach
Four quarter-size FE models with load and geometry symmetry conditions were constructed, the load-bearing capacity and associated mid-span deflections at critical points are verified against the full-scale experimental RC beams available in the literature. These developed FE models incorporated the tension stiffening effects and bond–slip behaviour. Theoretical analyses based on Indian standard code IS: 456–2000 and ACI 318–19 were also carried to verify the experimental and numerical predicted moments at critical loading points.

Findings
The load-deflection curves predicted through FE models exhibit closer corroboration with the experimental curves throughout the loading history. The contour plots for deflections, concrete principal stresses, reinforcement yield stresses are satisfactorily predicted by the FE models, which reveal the complete information of nonlinear behaviour of RC beams. The developed model well captured the initial and progressive crack patterns at each load increments.

Practical implications
The FE modelling is an efficient, valid and economical tool that is an alternative to the expensive experimental program and can be used to explore, analyse and fully understand the nonlinear response of RC beams under static loadings.

Originality/value
The ultimate moment capacity evaluated based on ACI 318–19 code provision show a better correlation with the experimental data as compared to the IS: 456–2000 code provision. The ultimate loads and associated centre-span deflections predicted by RN-2, RN-3, RB-12 and RB-16 FE model show a discrepancy of 1.66 and –0.49%, –4.68 and –0.60%, –9.38 and –14.53% and –4.37 and 4.21%, respectively, against the experimental results, which reveals that the developed ANSYS FE models predict consistent results and achieved a reasonable agreement with the experimental data.
Influence of COVID-19 pandemic on local construction firms’ viability
Authors: Oladimeji Olubimbola 
Published: August 2021

Purpose
This paper aims to assess COVID-19 (C-19) pandemic influence in the 37 factors identified from extant literature as factors influencing the viability of local construction firms (LCFs).

Design/methodology/approach
A sample size of 65 staff of 31 LCFs that were awarded construction projects contracts in institutions in Nigeria was purposefully selected and accessed based on relevant predetermined criteria. Respondents’ views on factors determining the viability of LCFs were obtained. Factors known to be influenced by C-19 are 25 of the 37 factors rated on a five-point Likert scale of importance by the respondents. Mean scores were used to rank the factors and principal component analysis was used to obtain key component factors (CFs) influenced by the C-19 pandemic.

Findings
Six of the first ten “extremely important” and “very important” factors are known to be influenced by C-19 pandemic. A total of 8 CFs having 20 variables with factor loadings of more than 0.5 each were known to be influenced by C-19. The C-19 pandemic influenced LCFs’ cash flow and management of construction labour, plant and equipment amidst variables that had above 0.8 factor loading.

Research limitations/implications
A limitation of this study is the inability to conduct close contact interview during this period to obtain personal views on the influence of C-19 on LCFs. However, this does not reduce the quality of findings of this study, as there are valid literature basis hinging this study findings.

Practical implications
The paper recommends that all stakeholders pay prompt attention to the factors adversely affected by the C-19 pandemic to improve or at the least sustain the viability of LCFs.

Originality/value
This paper fulfils a present pertinent need of assessing the influence of the C-19 pandemic on various factors influencing the viability of construction firms.
Viewpoint – a big opportunity for interactive marketing post-COVID-19
Authors: McDonald Malcolm 
Published: August 2021

PurposeThe objective of this short research-informed, practitioner-orientated paper is to provide a viewpoint on interactive marketing pre and post COVID-19.Design/methodology/approachThis is a conceptual article designed to articulate a viewpoint that despite COVID-19 the strategic considerations that marketers need to make remain unchanged.FindingsAs a result of the pandemic, there is much more obvious use of interactivity in other, aligned functions such as sales and customer services. The effectiveness of interactive marketing will only be increased by having focus on the fundamental roles of the marketing discipline, strategy and segmentation, and understanding customers at the individual level. We also need reminding briefly that the rules of competition had changed well before the COVID-19 pandemic. The traditional make and sell model has been substantially replaced by new technology-enabled organisations without the restrictions of high fixed costs and cloying bureaucracy. Customers can now search for and evaluate products and services independent of suppliers and now have as much information about suppliers as suppliers have of customers. This is the backcloth against which marketers face the challenges at the beginning of 2021. We conclude that there is a great future ahead. There is no reason why the best of interactive marketing will not be capable of building relationships that are great for customers, great for the people who create them, great for all stakeholders and great for the environment.Originality/valueThis is a practitioner viewpoint outlining the view that COVID-19 has not had as great of an impact on interactive marketing practice as technological change has and that interactive marketing will continue to develop after COVID-19 has receded.
Dialogic accounting through popular reporting and digital platforms
Authors: Grossi Giuseppe , Biancone Paolo Pietro , Secinaro Silvana , Brescia Valerio 
Published: August 2021

Purpose
The purpose of this study is to explore the usefulness of popular reporting (PR) in an Italian city as a dialogic accounting tool for promoting citizens’ engagement with digital platforms. This study aims to contribute to the debate on democratic accounting technologies with a focus on PR and digital platforms, using the theoretical lens of dialogic accounting.

Design/methodology/approach
A longitudinal case study is used to analyse the implementation and evolution of PR in the city of Turin, Italy and explore how the city involved its citizens with digital platforms.

Findings
This study contributes to the debate on public accountability through dialogic accounting tools.

Research limitations/implications
Multiple sources (surveys, interviews and interventionist workshops) are used to analyse Turin, Italy as a longitudinal case study.

Practical implications
This study offers practical reflections for legislators, politicians and public managers who need new knowledge and empirical analysis of the effective implementation of the PR as a tool for dialogue and empowering public accounting to hold continuous dialogue with the citizens.

Originality/value
PR can be considered a useful dialogic accounting tool for politicians, managers and government experts to encourage citizens’ engagement in a pluralistic society.
Toward a taxonomy of dynamic capabilities – a systematic literature review
Authors: Leemann Niklaus , Kanbach Dominik K. 
Published: August 2021

Purpose
This paper aims to categorize and organize dynamic capabilities that have been inductively identified in empirical research into a comprehensive taxonomy. Thus, it addresses calls in the literature for a better understanding of dynamic capabilities and integration of scattered empirical findings into theory.

Design/methodology/approach
A systematic literature review approach was adopted, with a total of 34 articles published between August 2007 and April 2020, from which 240 idiosyncratic dynamic capabilities were identified. The taxonomy was constructed using the Gioia-method.

Findings
The main finding is a three-level taxonomy of dynamic capabilities (DC). Level DC-1 is based on the existing triad of sensing, seizing and transforming. Level DC-2 is newly introduced to the literature by this study, consisting of 19 dynamic sub-capabilities that categorize and organize all 240 idiosyncratic dynamic capabilities in the sample (level DC-3). The taxonomy supports the existing claim that dynamic capabilities are common in key features and idiosyncratic in details. Moreover, theoretical connections to business model innovation and ambidexterity are indicated.

Practical implications
This study integrates scattered empirical findings of specific dynamic capabilities and translates them to a practitioner audience. The taxonomy allows the strategic manager to understand what they specifically are and, thus, assess the dynamic capability endowment of the firm which allows deploying, developing and fostering them.

Originality/value
The taxonomy provides a comprehensive and tangible picture of what dynamic capabilities look like in practice. It improves existing knowledge and understanding by bridging the rigor-relevance gap between rather rigorous conceptual literature and rather relevant empirical research as it integrates them. As such, it can serve as a “map” of dynamic capabilities for scholars and practitioners.
Novel study of using chitosan in gamma dose monitoring applications
Authors: Bekhit Mohamad , Fahim Essam , Sobhy Asmaa 
Published: August 2021

Purpose
The purpose of this paper is to fall light on the possibility of using the biopolymer chitosan in gamma dose monitoring.

Design/methodology/approach
The chitosan films were irradiated to gamma rays in the range starting from 10 to 120 kGy at a dose rate of 1.4 kGy/h using 60Co gamma source. The ultra violet and visible (UV/Vis) spectrophotometry were used to examine the optical properties of chitosan film. Also, Fourier transform infrared (FTIR) analysis was used to detect and trace any change in structural bands that may take place upon irradiation.

Findings
Increase in optical density of the chitosan film was recorded at 298 nm that correlated with increasing in the absorbed doses. Change in color of the film from pale yellow to denser yellow was detected upon increasing the absorbed doses. The close investigation for UV/Vis and FTIR analysis nominates the chitosan film to be used as a label-dosimeter in the range of 10–120 kGy depending on chitosan concentrations. The chitosan film has an excellent stability in different environmental conditions with ±3.7% uncertainty in measurements (2σ, approximately equal to a 95% confidence level).

Research limitations/implications
Chitosan film may be used as a medium and high-dose monitor with an acceptable overall uncertainty in routine radiation processing

Originality/value
The useful dose range from 10 to 80 kGy was detected for different concentrations of chitosan (0.5, 1, 1.5 Wt%) that correlated with increasing the absorbed dose, which is assigned to the linear parts in the target response curves. For the dose range 10–120 kGy, the film may be used as label dosimeter with detected color change from pale yellow to dense yellow.
The informal culture of a direct care staff team supporting people with intellectual disabilities who present with behaviours that challenge: commentary
Authors: Beadle-Brown Julie 
Published: August 2021

Purpose
The purpose of this paper is to provide a commentary on Banks et al. and sets the wider context.

Design/methodology/approach
It provides a brief narrative review of the literature on the factors that determine the quality of life of people with intellectual disabilities, including the impact of culture.

Findings
Key to ensuring good quality of life outcomes is support that is facilitative, enabling and empowering which can compensate for severity of disability and improve people’s experiences. This approach is called Active Support. Improving quality of life is a key part of preventing and responding to behaviours that challenge. Culture is an important factor in ensuring staff are motivated to work in such ways but is likely to be intertwined with many other factors.

Originality/value
This paper summarises the key literature on what is needed to improve outcomes for people with intellectual disabilities. It introduces the importance of exploring how the different dimensions of culture interact and how culture, practice, processes and structures might work in a much more complex and intertwined fashion than previously conceptualised.
Making the world a better place: achieving impact through innovation and an entrepreneurial ethos
Authors: Pepin John 
Published: August 2021

Purpose
The purpose of this paper is to describe and evaluate the programme of philanthropy arising from the work of the Shirley Foundation, with particular reference to its impact in addressing issues related to autism.

Design/methodology/approach
The paper is based on the 2017 evaluation of The Shirley Foundation 1996–2016 by Aperio Group (Europe) Limited entitled 20 Years of Grant Making Autism and Information Technology: An Overview of The Foundation’s Impact (Pepin, 2017). The evaluation included interviews with Dame Stephanie Shirley CH (the Foundation’s founder), a documentation review and a survey of key grantees.

Findings
The evaluation concluded that the funding achieved both grantee purposes and those of The Shirley Foundation. The projects funded generally produced outcomes that made a difference and grant-making and monitoring processes were regarded, on the whole, as efficient.

Originality/value
The evaluation demonstrates the value of an innovative, catalytic, social entrepreneurial approach to funding to achieve impact.
Sleep disturbance in clients attending a specialist addiction clinic
Authors: Ogeil Rowan P. , Arunogiri Shalini , Petersen Vanessa , Gooden James R. , Lubman Dan I. 
Published: August 2021

AbstractBackground and ObjectivesSleep problems are common among clients attending alcohol and drug services, yet the specific components of sleep disturbed by primary drug of concern (PDOC), and their relationships to affective disorder symptoms are unclear.MethodsWe examined sleep problems in clients (n = 32) attending a specialist addiction clinic.ResultsGlobal sleep quality was rated poor by >90% of participants (particularly disturbances, latency and efficiency components), with significant associations (p < .05) between poor sleep quality and depression (r = .517), anxiety (r = .571) and stress (r = .503). Sleep quality was significantly poorer among those with a nonalcohol PDOC compared with alcohol as PDOC, t(22) = 3.09, p = .005.Conclusion and Scientific SignificancePoor sleep is almost ubiquitous among clients attending alcohol and drug services. However, components of sleep quality disturbed differ in terms of PDOC, highlighting the need for individualised sleep interventions.