bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Sintering Behaviors on Dimensional Accuracy, Surface Quality, and Mechanical Properties of Stereolithography-printed 3Y-ZrO2 Ceramics
Authors: Zhang Cheng , Jiang Zhaoliang , Zhao Li , Guo Weiwei , Zhang Chengpeng 
Published: June 2021

Abstract
Sintering process is essential to acquire the final components by stereolithography (SLA), which is a promising additive manufacturing technology for the fabrication of complex, custom-designed dental implants. 3Y-ZrO2 ceramics at different sintering behaviors in air atmosphere were successfully obtained in this study. Firstly, the curing properties of homemade pastes were studied, and the penetration depth and critical exposure of the pastes were calculated as 17.2 μm and 4.80 mJ/cm2, respectively. The green ceramic parts were performed at 154 mW laser power and 6000 mm/s scanning speed. Then, the dimensional accuracy, surface quality, and mechanical properties of 3Y-ZrO2 ceramics were investigated. The shrinkages of length, width, and height were 26%~27 %, 30%~31 %, and 27%~33 % in sintered ceramics, respectively. The Ra values of XOY, YOZ, and XOZ surfaces showed an anisotropic feature, and they were smallest as 0.52 μm, 2.40 μm, and 2.46 μm, respectively. Meanwhile, the mechanical properties presented a similar trend that they grew first and then dropped at various sintering behaviors. The optimal parameters were 1500 ℃, 60 min, and 4 ℃/min, and the maximum relative density of 96.18 %, Vickers hardness of 12.45 GPa, and fracture toughness of 6.35 MPa·m1/2 were achieved. Finally, the X-ray diffraction (XRD) and energy-dispersive spectroscopy (EDS) analysis demonstrated that no change was observed in crystal transformation and phase composition, and the organic was completely removed in sintered ceramics. This research is expected to provide a technical guide for the fabrication of ceramics for dental implants using SLA technique.
Construction of a Pseudogene‐associated ceRNA Network and Identification of Prognostic Pseudogene Biomarkers for Colorectal Cancer
Authors: Li Zhuoqi , Zhou Jing , Gu Liankun , Zhang Baozhen 
Published: June 2021

Abstract
Colorectal cancer (CRC) is one of the most common and deadly malignant carcinomas. Many long noncoding RNAs (lncRNA) have been reported to play an important role in the tumorigenesis of CRC by interacting with miRNAs and influencing the expression of some mRNAs through a competing endogenous RNA (ceRNA) network. Pseudogenes are one kind of lncRNA and can act as RNA sponges for miRNAs and regulate gene expression via ceRNA networks, but there are few studies about pseudogenes in CRC. In this study, total of 31 differentially expressed (DE) pseudogenes, 17 DE miRNAs and 152 DE mRNAs were identified by analyzing the expression profiles of colon adenocarcinoma (COAD) obtained from The Cancer Genome Atlas (TCGA). And a ceRNA network was constructed based on these RNAs. Kaplan–Meier analysis showed that 7 pseudogenes, 4 miRNAs and 30 mRNAs were significantly associated with overall survival. Then multivariate Cox regression analysis on the ceRNA-related DE pseudogenes was performed and a 5-pseudogene signature with the greatest prognostic value for CRC was identified. What’s more, the results were validated by the Gene Expression Omnibus (GEO) database, and quantitative real‐time PCR (qRT‐PCR) in 113 pairs of CRC tissues. In conclusion, this study provides a pseudogene-associated ceRNA network and 7 prognostic pseudogene biomarkers, and a 5-pseudogene prognostic risk signature that may be useful to predict the survival of CRC patients.
Suspicion of Frasier's syndrome in the nephrology unit of the Internal Medicine department of the HUEH: Case study and review of the literature.
Authors: PAUL Axler JEAN , LOUIS Dieuguens , DESRAVINES Ansly Jefferson , JEAN Raema Mimrod , BAPTISTE Alfadler JEAN , BUTEAU Jean Henold , ANDRE Wislet 
Published: June 2021

Abstract
ObjectiveFrasier syndrome is a rare genetic nephropathy characterized by the presence of progressive glomerulopathy with proteinuria associated with male pseudo hermaphroditism. This case study described a picture of a young boy where the clinical suspicion context reminded the Frasier Syndrome. To our knowledge, this case is the first described in Haiti.Case studyThis is a 19 years old young phenotypically male, born with a genital anomaly, was seen on referral at the nephrology/dialysis unit of the internal medicine department of HUEH for evaluation and follow-up. Insidious progression of symptoms had occurred over 3 years. Over three months of outpatient follow-up, he had four sets of renal labs drawn, and all showed impaired renal function. At the ultrasound a bilateral cryptorchidism is described in the inguinal, and presence of functional ovaries with follicles of variable size scattered in the parenchyma. So, in the light of these anamnestic, clinical and paraclinical findings we concluded to the diagnosis of end-stage renal failure by progressive glomerulopathy in a context of Frasier's syndrome.ConclusionWith any clinical picture consisting of genital anomalies at birth, renal symptomatology during childhood and the diagnosis of renal failure during adolescence, rare genetic nephropathies, such as Frasier syndrome must be considered.
Artemether Targets Raptor-Induced Actin Polymerization and Suppresses Migration of Fibroblast-like Synoviocytes in Rheumatoid Arthritis
Authors: Ma Jian-Da , Jing Jun , Wang Jun-Wei , Zhang Xue-Pei , Li Qian-Hua , Mo Ying-Qian , Lin Jian-Zi , Luo Man-Li , Dai Lie 
Published: June 2021

Abstract
Background: Fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA) may cause articular damage as a result of its aggressive features including direct adhesion and invasion of surface cartilage in joints. Artemether (ART), one of the artemisinin derivatives with antimalarial properties, showed inhibitory effect on inflammation and destruction of joints in collagen-induced arthritis rats, which might be applied in RA treatment. However, whether ART has effects on the aggressive properties of human RA-FLS remains unexplored. Methods: Synovium was obtained from patients with active RA (n=18) and FLS were isolated in vitro. RA-FLS were subjected to cell migration, invasion assays, live-cell imaging analysis and Rho GTPase activation assay after ART treatment. To identify the therapeutic target of ART, key signaling molecules of PI3K/Akt, AMPK, MAPK, NF-κB and mTOR pathways from RA-FLS were examined by Western Blot after ART treatment. Raptor was knockdown or overexpressed by siRNA or lentivirus transfection to reveal its role on regulating the aggressive properties of RA-FLS.Results: ART treatment significantly suppressed the transwell migration and invasion of synovial FLS from RA patients. Time-lapse microscopy revealed that ART treatment reduced random migration velocity of RA-FLS, as well as the directional persistence. ART also impaired the formation of filopodia and lamellipodia in RA-FLS. Further mechanism investigation showed that ART reduced the protein level of Raptor, a critical component of the mTOR pathway, and its downstream target 4E-BP1. It also inhibited the activation of Rho GTPases and the expression of actin binding proteins, including Profilin 1 and p-Cofilin. Raptor overexpression could reverse the anti-migration and anti-invasion effects of ART on RA-FLS as well as the suppression of Rho GTPases activation and the expression of actin binding proteins. Conclusion: ART can inhibit migration of RA-FLS by blocking Raptor-induced actin polymerization. ART might be a potential agent targeting FLS in RA treatment.
Mitochondrial Membrane Potential Influences Aβ Production and Amyloid Precursor Protein localization
Authors: Wilkins Heather , Troutwine Benjamin , Menta Blaise , Manley Sharon , Strope Taylor , Lysaker Colton , Swerdlow Russell 
Published: June 2021

Abstract

Beta amyloid (Aβ), which derives from the amyloid precursor protein (APP), forms plaques and serves as a fluid biomarker in Alzheimer’s disease (AD). How Aβ forms from APP is known, but questions relating to APP and Aβ biology remain unanswered. AD patients show mitochondrial dysfunction and an Aβ/ APP/ mitochondria relationship exists. Here, we considered how mitochondrial biology may impact APP and Aβ biology. We showed that mitochondrial depolarization routes APP to, while hyperpolarization routes APP away from, the organelle. Mitochondrial APP and cell Aβ secretion inversely correlate, as cells with more mitochondrial APP secrete less Aβ, and cells with less mitochondrial APP secrete more Aβ. Overall, our findings indicate mitochondrial function alters APP localization and suggest enhanced mitochondrial activity or factors associated with enhanced mitochondrial activity promote Aβ secretion while depressed mitochondrial activity or factors associated with depressed mitochondrial activity minimize Aβ secretion. Our data complement other studies that indicate a mitochondrial, APP, and Aβ nexus, and could help explain why cerebrospinal fluid Aβ is lower in those with AD. Our data further suggest Aβ secretion could serve as a biomarker of cell or tissue mitochondrial function.
Deciphering the interactions of SARS-CoV-2 proteins with human ion channels using machine learning-based method
Authors: Munjal Nupur S. , Sapra Dikscha , Goyal Abhishek , Parthasarathi K.T. Shreya , Pandey Akhilesh , Banerjee Manidipa , Sharma Jyoti 
Published: June 2021

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the worldwide COVID-19 pandemic which began in 2019. It has a high transmission rate and pathogenicity leading to health emergencies and economic crisis. Recent studies pertaining to the understanding of the molecular pathogenesis of SARS-CoV-2 infection exhibited the indispensable role of ion channels in viral infection inside the host. Moreover, machine learning-based algorithms are providing higher accuracy for host-SARS-CoV-2 protein-protein interactions (PPIs). In this study, predictions of PPIs of SARS-CoV-2 proteins with human ion channels (HICs) were performed using PPI-MetaGO algorithm. The PPIs were predicted with 82.71% accuracy, 84.09% precision, 84.09% sensitivity, 0.89 AUC-ROC, 65.17% MCC score and 84.09% F1 score. Thereafter, PPI networks of SARS-CoV-2 proteins with HICs were generated. Furthermore, biological pathway analysis of HICs interacting with SARS-CoV-2 proteins showed the involvement of six pathways, namely inflammatory mediator regulation of TRP channels, insulin secretion, renin secretion, gap junction, taste transduction and apelin signaling pathway. The inositol 1,4,5-trisphosphate receptor 1 (ITPR1) and transient receptor potential cation channel subfamily A member 1 (TRPA1) were identified as potential target proteins. Various FDA approved drugs interacting with ITPR1 and TRPA1 are also available. It is anticipated that targeting ITPR1 and TRPA1 may provide a better therapeutic management of infection caused by SARS-CoV-2. The study also reinforces the drug repurposing approach for the development of host directed antiviral drugs.
Quality of water for reprocessing of medical devices in healthcare facilities in Nepal
Authors: Panta Gopal , Richardson Ann K. , Shaw Ian C. 
Published: June 2021

Abstract
Reusable medical devices are decontaminated and sterilized often many times by healthcare facilities across the globe. Reprocessing of medical devices comprises several processes and water plays an important role in some of these, including cleaning and steam sterilization. The water used is required to have certain qualities to ensure the effectiveness of the processes. In this short communication, we report findings of our study which measured quality parameters (pH, total hardness) for water used for medical device reprocessing in 13 primary and secondary care public hospitals in Nepal. The mean pH of water used for reprocessing of medical devices varied from 6.48 to 8.05 across the hospitals whereas the mean total hardness of water varied from 5.93 to 402.50 mg/L CaCO3. Although the range of the mean water pH across hospitals fell within the recommended range, many of the hospitals had mean total hardness higher than recommended for cleaning medical devices. None of the hospitals had mean total hardness suitable for using as feed-water for steam generation. Public hospitals in Nepal should have appropriate water treatment systems so that the recommended water quality can be achieved to ensure effective decontamination and reprocessing of medical devices.
Testing of a novel IFAS-MBR process with co-precipitation
Authors: Rusten B. , Rathnaweera S. S. , Manamperuma L. D. , Kjeverud M. , Ødegaard H. 
Published: June 2021

Abstract
IFAS-MBR with co-precipitation, not yet commonly used in practice, will result in a very compact process for nutrient removal. The process, based on a combined pre- and post-denitrification IFAS process with membrane separation (IFAS-MBR), was tested in two parallel small-scale plants. Train A was operated with co-precipitation in order to achieve high removal of total P (TP). Train B, without co-precipitation, served as a control. Due to the coagulant (Al) addition, the concern was precipitation on the biofilm carriers in the aerobic reactor in Train A. A small internal air-lift pump proved to be very efficient in controlling biofilm thickness and removing excess biofilm mass as needed. A coagulant dose equivalent to an Al/TP molar ratio of 1.9 was necessary to achieve 99% TP removal and 0.10 mg TP/l in the effluent of Train A. Very good removal of total N was achieved in both trains. Train A had a biofilm nitrification rate of 0.65 g NH4-N/m2d at 12–13 °C and 5.2–5.6 mg O2/l. The tests demonstrated that an IFAS-MBR process with co-precipitation and an aerobic suspended biomass SRT of 5–10 days is feasible, and that all the performance goals set up for the full-scale plant were achieved.
Development of hollow δ-FeOOH structures for mercury removal from water
Authors: Maia Luiz F. O. , Lages Guilherme , Ladeira Patricia C. C. , Batista Bruno Lemos , Faria Márcia C. S. , Oliveira Luiz C. A. , Pereira Márcio C. , Rodrigues Jairo Lisboa 
Published: June 2021

Abstract
δ-FeOOH, a magnetic iron oxyhydroxide, has a significant number of -OH groups on its surface. These provide an attractive platform for heavy metal species in contaminated water, giving it potential as an adsorbent. Its performance can be improved by increasing the number of active surface sites. δ-FeOOH hollow structures were synthesized on a mesoporous silica surface then treated with NaOH solution. X-ray diffraction (XRD) and transmission electron microscopy (TEM) confirmed that structure synthesis was successful. δ-FeOOH, 5,27 nm, hollow crystals were produced with 63 m2 g−1 surface area and 20 nm average pore size. The point of zero charge was 4.72, which is beneficial for Hg(II) adsorption near neutral pH. The maximum Hg(II) adsorption capacity at pH 7 was determined as 89.1 mg g−1. The kinetics data were best fitted by a pseudo-second-order model with k2 equal 0,1151 g mg−1min−1. Finally, a nanomaterial filter was developed and used to remove mercury in water samples from a Brazilian river.
In Vitro Evaluation of Immunogenicity of Recombinant OMP25 Protein Obtained from Endemic Brucella abortus Biovar 3 as Vaccine Candidate Molecule Against Animal Brucellosis
Authors: Atabey Tuğba , Acar Tayfun , Derman Serap , Ordu Emel , Erdemir Ayşegül , Taşlı Pakize Neslihan , Gür Günseli Kurt , Şahin Fikrettin , Güllüce Medine , Arasoğlu Tülin 
Published: June 2021

Background:
Brucellosis is a zoonotic disease that causes serious economic losses due
to factors, such as miscarriages and decreased milk yield in animals. Existing live vaccines have
some disadvantages, so effective vaccines need to be developed with new technological approaches.

Objectives:
The primary objectives of this study were the expression and purification of recombinant
Omp25 fusion protein from B. abortus, and the evaluation of the effect of the Omp25 protein
on cell viability and inflammatory response.

Methods:
The omp25 gene region was amplified by a polymerase chain reaction and cloned into a
Pet102/D-TOPO expression vector. The protein expression was carried out using the prokaryotic
expression system. The recombinant Omp25 protein was purified with affinity chromatography followed
by GPC (Gel Permeation Chromatography). The MTS assay and cytokine-release measurements
were carried out to evaluate cell viability and inflammatory response, respectively.

Results:
It was determined that doses of the recombinant Omp25 protein greater than 0.1 μg/mL
are toxic to RAW cells. Doses of 1 μg/mL and lower significantly increased inflammation due to
Nitric Oxide (NO) levels. ELISA results showed that IFN-γ was produced in stimulated RAW
264.7 cells at a dose that did not affect the viability (0.05 μg/mL). However, IL-12, which is
known to have a dual role in the activation of macrophages, did not show a statistically significant
difference at the same dose.

Conclusion:
Studies on cell viability and Th1-related cytokine release suggest Omp25 protein to
be a promising candidate molecule for vaccine development.
Santacruzamate A Compositions, Analogs and Methods of Use: A Patent Evaluation of WO 2014/018913 (A2)
Authors: Zhang Lin , Zhang Lei 
Published: June 2021

Background:
Santacruzamate A (SCA) is a natural product isolated from a marine
cyanobacterium. Activity test results revealed that SCA is a highly potent HDAC2 inhibitor with
an IC50 value of 0.112 nM. The IC50 of SCA in inhibiting cancer cell proliferation is 28.3 μM and
1.3μM on HCT116 and HuT-78 cells, respectively.

Objective:
To develop HDAC inhibitors with improved activity, SCA analogs were synthesized for
the structure-activity relationship (SAR) studies.

Method:
Various substituted groups were introduced into the zinc binging group, linker, and cap
regions of SCA by various chemical synthetic methods.

Result:
Compared with SCA, the derivatives of SCA did not exhibit improved HDAC2 inhibitory
activity. Nevertheless, several molecules such as III-32, III-33, IV-4b, and IV-11 showed improved
activity in inhibiting cell proliferation on HCT116 and HuT-78 cells.

Conclusion:
Collectively, a potent HDAC2 inhibitor SCA was discovered as a lead compound for
further development of selective HDAC inhibitors.
A Blockchain based Fund Management Scheme for Financial Transactions
in NGOs
Authors: Pandey Dhiraj , jain Megha , Kaswan Suresh 
Published: June 2021

Background:
In the world of the latest technologies, the blockchain is one of the
popular techniques for stopping fraudulent activities. Non-Government Organization (NGO) is
increasingly being used to support all the needy people across the globe to shape the world’s
responsibility towards society for sustainable development. The existing method of donating
money and its monitoring is facing a major corruption problem in several world-renowned NGOs.
A Blockchain can transform the way the current business of money transaction is being done in
NGOs. The blockchain-based application works on the concept of a decentralized system.

Methods:
This article presents a blockchain-based transaction system to prevent corruption and
money laundering in NGOs and government fundraising organizations. A smart contract has
been designed to stop any illegitimate block changes during a financial transaction. Since every
node has a copy of the ledger, so it is very difficult to perform malicious activity. Furthermore,
the donator can watch how the money flows in the different transactions and everyone can
browse the account history. An evaluative judgment, comparing with various consensus algorithms,
has also been presented along with their complex nature. The decentralized approach
has eliminated the chance of a single point of failure which in turn makes the system robust.

Results:
The developed framework for the financial transaction using blockchain has been tested
using the Rinkeby Test Network. The generators and campaign contracts have been developed
and deployed in the Rinkeby testing network. The results indicate that the computing is
much more secure and free from the scam in comparison to the traditional client-server financial
transaction system.

Conclusion:
Finally, the proposed approach suggests scenarios such as in NGOs where the introduced
security approach should prove to be adequate.
CEO Compensation, Incentive Alignment, and Carbon Transparency
Authors: Luo Le , Wu Hongjun , Zhang Chuyue 
Published: June 2021

ABSTRACT
We examine whether chief executive officer compensation aligned with stakeholders' interests is associated with enhanced corporate carbon transparency. Using an international sample obtained from the CDP, we find that corporate carbon transparency—as measured by both the propensity to voluntarily disclose carbon information and the quality and comprehensiveness of the disclosure—is greater when managers' compensation contracts are better aligned with stakeholder interests. Further analyses indicate that this positive relationship is stronger in countries or regions with a code law legal system, with an inefficient rule of law, that show strong social norms toward climate change, that feature collectivist societies, and that have a long-term orientation. These findings indicate that the stakeholder agency problem of voluntary carbon disclosure can be addressed through executive incentives that are aligned with stakeholders' interests.
The Relationship between Workload and Performance of Research University Academics in Malaysia: The Mediating Effects of Career Commitment and Job Satisfaction
Authors: Janib Jamali , Mohd Rasdi Roziah , Omar Zoharah , Alias Siti Noormi , Zaremohzzabieh Zeinab , Ahrari Seyedali 
Published: June 2021

Abstract: The purpose of this study is to test the relationship between job workload and academic performance among university academic staff in Malaysia. The paper also attempts to discuss and then seek empirical evidence to the two mediational paths (namely, career commitment and job satisfaction) that explain the focal relationship between job workload and academic performance. To test the three proposed hypotheses, the study used cross-sectional data collected from academic staff serving in Malaysian Research Universities (MRUs). The final sample of 191 valid and complete responses was analysed using SmartPLS 3.3.3 to test the hypotheses. Results showed that workload is negatively related to academic staff performance. In addition, job satisfaction mediates workload and academic staff performance linkage. These findings reinforce the importance of job satisfaction as an influencing factor against the deleterious effect of job workload and academic staff performance. The study has shown that, contrary to our expectations, career commitment does not mediate the relation between job workload and academic staff performance. Going forward, this study provides new insights about the effect of job workload on the performance of university academic staff through intervening variables.

Keywords: Career commitment, Job satisfaction, Job workload, Academic staff performance, Malaysian Research University