bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE MULTI-CRITERIA PLANNING MODEL OF URBAN SPATIAL DEVELOPMENT WITH REGARD FOR THE INTERESTS OF STAKEHOLDERS
Authors: Tymoshchuk М. , Sosnova N. , Feshchur R. , Shyshkovskyi S. , Kopytko S. 
Published: December 2021

Abstract. The present study is aimed at supporting the concept and making the model of urban spatial development as a complex architectural town-building and socio-economic system, as well as developing tools for reaching agreed multi-criteria planning decisions in the interests of stakeholders.
The study examines basic theories (such as pattern language and central places) and conceptual approaches to the formation of urban spaces of trade, recreational, healthcare, image, entertainment, environmental, and sports character. It reveals the possibility and substantiates the necessity of employing expert methods and methods of project management for analysing, generalising, and modelling of urban civic space planning. In addition, there search substantiates conceptual principles, sets out general requirements, and highlights the main tasks of decision-making planning.
Furthermore, a multi-criteria optimization model has been built to plan urban spaces in conditions of contradictory interests of stakeholders, under limited resources and in complete in formation. The implementation of the model is to increase the validity of planning decisions and, as a result, lead to higher standards of living due to expansion of existing and diversification of potential functions of urban public spaces. For the evaluation of architectural urban building, economic, social, cultural-monumental, and ecological results, the study lays down criteria depending on the extent the plan of urban public space development is put into action.The method of successive concessions has been shown as an efficient tool for reaching agreed co-created planning decisions by stakeholders.
The provided basic model of reaching optimum planning decisions satisfying certain criteria contains restrictions on potential urban spatial development, as well as on the sequence in which interconnected development projects are carried out, and on the balance between supply and demand for services (functions) of urban public spaces. The model, which has been modified by the method of successive concessions for achieving agreed planning decisions in the interests of stakeholders, is based on establishing a dominant optimization criterion, on expert justifying of allowable optimality losses against initial planning decisions per criterion at each stage of subsequent calculations, and on reaching a consensuson a final decision.
Keywords: public space, a planning decision, spatial development, evaluation criteria, development potential, a model, interests of stakeholders, a co-creation method.
JEL Classification I00, L51, L83, Z10
Formulas: 16; fig.: 2; tabl.: 2; bibl.: 23.
LED Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer Optimizes Wound Repair in Rats
Authors: Silva Naldiana Cerqueira , De Alexandria Francisco Eugênio Deusdará , Assis Livia , Filho Antonio Luiz Martins Maia , Ferreira Rui Seabra , Barraviera Benedito , Parizotto Nivaldo Antonio , Silva José Figueredo , Tim Carla Roberta 
Published: December 2021

Abstract
This study aimed to evaluate the effect of LED photobiomodulation therapy associated or not with heterologous fibrin biopolymer to repair skin wounds in rats. Full-thickness skin wounds were induced on the back of 84 Wistar rats and they were randomly divided into 4 groups: control group (CG), LED group (LED), heterologous fibrin biopolymer group (HFB), and LED + heterologous fibrin biopolymer group (LED + HFB). The animals were euthanized at 7, 14, and 21 days after surgery. In the macroscopic findings, it was observed in the control group, mild local inflammation, evidenced between the 5th and 6th day after the injury, not manifested in the other groups. The analysis of the wound reduction index shown that the treated groups presented greater wound reduction in the three periods evaluated. The results of the degree of inflammation showed that the LED and LED + HFB groups showed a predominance of chronic inflammation, while the control group showed a predominance of diffuse acute inflammation. The quantity of type I collagen it was bigger after 7 days in the LED and LED + HFB groups, and at 14 and 21 days this type of collagen appears more intensely in the group that used only HFB. The traction force at 7 days of the experiment, the treated groups showed better performance, while at 14 and 21 days the group that used only the biopolymer obtained greater strength in the suture region. Results have indicated that treatments using HFB and LED, either isolated or associated, stimulated the wound repair process in rats. HFB promote collagen maturation.
The Impacts of G4S Mutation on N-glycosylation and conformation of the Human Coagulation Factor IX GLA domain: In silico and In vitro Analysis
Authors: Ghasemi Fahimeh , Maddah Mina , Kalhor Hourieh , Khorashadizadeh Mohsen , Zomorodipour Alireza 
Published: December 2021

Abstract
Missense mutations are the most prevalent form of mutation in hemophilia B patients. These alterations may result in the creation of novel and non-native N-glycosylation sites (Asn-X-Ser/Thr) through single amino acid substitutions. The pathogenic mechanisms of N-glycosylation mutations in hemophilia B patients have not been extensively studied yet. By survey among known missense mutations, we found only one N-glycosylation mutation in the γ-carboxyglutamic-rich (GLA) domain of the human coagulation factor IX (hFIX). This mutation that was reported in patients with mild and moderate hemophilia B, is caused by G4S amino acid substitution. To investigate the possibility of glycan attachment to the novel N-glycosylation site in G4S-mutant hFIX and the occurrence of hyperglycosylation, site-directed mutagenesis was applied to introduce the selected mutation into the coding sequence of the hFIX. The nucleotide sequences of the both native and G4S-mutant hFIX were separately cloned into the pcDNA3.1 expression plasmid and transiently expressed in HEK293T cells. Our results from gradient SDS-PAGE and western blotting analysis of the both recombinant native and mutant hFIX demonstrated no glycan attachment to the new N-glycosylation site in the G4S-mutant hFIX. Molecular dynamics (MD) simulation was also conducted to provide atomistic insights into structure and behavior of the native and G4S-mutant GLA domains in the both free and membrane-bound states. The results revealed that the mutation slightly affected the dynamic behavior of the mutant GLA domain. The conformational analysis proved that the native GLA domain had less fluctuation and more stability than the mutant GLA domain. The slight conformational changes may influence the binding capacity and interaction of the mutant GLA domain to phospholipid bilayer which is necessary for coagulation activity of the hFIX. These findings were in accordance with the nature of the G4S mutation which causes mild hemophilia B.
Pancreatic α and β cells are globally phase-locked
Authors: Ren Huixia , Li Yanjun , Han Chensheng , Yu Yi , Shi Bowen , Peng Xiaohong , Wu Shufang , Yang Xiaojing , Sneppen Kim , Chen Liangyi , Tang Chao 
Published: December 2021

Abstract
The Ca2+ modulated pulsatile secretion of glucagon and insulin by pancreatic α and β cells plays a key role in glucose homeostasis. However, how α and β cells coordinate via paracrine interaction to produce various Ca2+ oscillation patterns is still elusive. Using a microfluidic device and transgenic mice in which α and β cells were labeled with different colors, we were able to record islet Ca2+ signals at single cell level for long times. Upon glucose stimulation, we observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. After a transient period, the oscillations of α and β cells were globally phase-locked, i.e., the two types of cells in an islet each oscillate synchronously but with a phase shift between the two. While the activation of α cells displayed a fixed time delay of ~20 s to that of β cells, β cells activated with a tunable delay after the α cells. As a result, the tunable phase shift between α and β cells set the islet oscillation period and pattern. Furthermore, we demonstrated that the phase shift can be modulated by glucagon. A mathematical model of islet Ca2+ oscillation taking into consideration of the paracrine interaction was constructed, which quantitatively agreed with the experimental data. Our study highlights the importance of cell-cell interaction to generate stable but tunable islet oscillation patterns.
Upcycling of Waste Polyethylene and Cement Kiln Dust to Produce Polymeric Composite Sheets Using Gamma Irradiation
Authors: Khozemy Ehab , Radi Hamdi , Mazied Nabila A 
Published: December 2021

Abstract
Cement kiln dust (CKD) is a residue produced during the manufacture of cement that contains hazardous solid waste of high toxicity that affects the environment and public health. In this study, the possibility of using cement waste as a filler in the plastic and rubber industry was studied. Different concentrations of (CKD) and gamma irradiation on the mechanical, thermal stability of the prepared composites sheets were investigated. Different concentrations of (CKD) 10, 15, 20, 30, 35, and 40 wt % were prepared with double screw extrusion by mixing waste polyethylene (WPE), de-vulcanized rubber (DWR), and EPDM rubber. These prepared composites were irradiated with doses 25, 50, 75, 100, and 150 kGy to study the effect of radiation on the physical, mechanical properties, and thermal stability of the prepared composite sheets. The prepared composite sheets were characterized and verified by FTIR and soluble fractions. The morphology of the composite sheets was investigated by SEM. Mechanical and thermal properties were investigated to evaluate the possibility of its application in the plastic and rubber industry.
A Flexible Broadband CPW-Fed Circularly Polarized Biomedical Implantable Antenna With Enhanced Axial Ratio Bandwidth
Authors: Ahmad Sarosh , Manzoor Bilal , Naseer Salman , Ghaffar Adnan , Hussein Mousa 
Published: December 2021

Abstract
Implantable antennas have a vital role in biomedical telemetry applications. Therefore, a compact low-profile circularly polarized biomedical implantable antenna operational in industrial, scientific, and medical (ISM) band at 2.45 GHz is reported. The presented antenna is fed by a modified co-planar waveguide (CPW) technique to keep the size of the antenna compact. The radiating monopole consists of a slotted rectangular patch with one slot at an angle of 45 degree and truncated small patch on the left end of the CPW ground plane to make the antenna circularly polarized at the required frequency band. A flexible Roger Duroid RT5880 substrate (εr = 2.2, tanδ = 0.0009) with the standard thickness of 0.254 mm is used to achieve bending abilities. The complete volume of the designed antenna is 21 mm × 13.5 mm × 0.254 mm (0.25 × 0.16 × 0.003 ). The antenna covers the bandwidth from 2.35-2.55 GHz (200 MHz) in free space while from 1.63 GHz to 2.8 GHz (1.17 GHz) inside skin tissue. As the designed antenna is operational in skin tissue with larger bandwidth, the bending analysis along the (x & y)-axis is also analyzed through the simulation. A good agreement between the simulation and measurements of the bended antenna is observed. The measured -10dB impedance bandwidth and the 3dB axial ratio (AR) bandwidth inside skin-mimicking gel are 47.7% and 53.8%, respectively at 2.45 GHz frequency band. Finally, the specific absorption rate (SAR) values are also analyzed through simulations, and it is 0.78 W/kg inside skin over 1 g of mass tissue. The proposed SAR values are less than the limit of the federal communication commission (FCC). This antenna is miniaturized and an ideal applicant for the biomedical implantable applications.
The Effect of A2E On The Levels of ROS and The Distribution of A2E in RPE Cells Exposed to Blue Light
Authors: Luo Maomei , Zeng Chun , Wang Shu , Cai Shanjun 
Published: December 2021

Abstract
AimsTo establish the N-retinylidene-N-retinylethanolamine(A2E) and blue light induced RPE cells damage model to explore the regularity of distribution of A2E and the levels of reactive oxygen species(ROS).MethodsThe fourth to sixth generation of human RPE cells in vitro were divided into five groups randomly: control group, blue light group, A2E-loaded group, A2E-loaed+blue light group and A2E-loaded+blue light +nifedipine group. The levels of ROS in cytoplasm by DCFH-DA staining was assayed by flow cytometry. The concentration of A2E in cytoplasm and lysosomes were assayed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). The fluorescence intensity of A2E in lysosomes by Lysotracker redDND-99 staining was assayed by confocal laser scanning microscope. ResultsExposure to blue light and/or A2E could increase the levels of ROS in RPE cells, and nifedipine could inhibit oxidative stress response and reduce ROS levels. By HPLC-MS, it was found that A2E was not detected in the groups without load A2E, and A2E levels in cytoplasm and lysosomes decreased after light exposure. The green fluorescence produced by A2E loaded on RPE cells was mostly coincident with the red fluorescence labeled by lysosomes.ConclusionBlue light and A2E can increase the ROS levels of RPE cells and both have a synergistic effect. A2E is mainly concentrated in lysosomes, which is reduced by oxidation under blue light irradiation, damages lysosomal membrane with oxidized species of A2E, and leaks out from lysosomes.
Identifying the Key Factors Influencing Technology Management in the Power Plant and Electric Power Industry, and Prioritizing Factors Using Network Analytical Process (ANP): Evidence from MAPNA Group
Authors: khamseh abbas , kheradranjbar maryam 
Published: December 2021

Abstract
This research has been conducted in order to identify and determine the order of influential factors on technology management in MAPNA Group. After studying the related literature and collecting conducted researches and experts’ opinions, 71 influential indicators were identified in the technology management of power plants and the electric power industry. Afterward, the opinions of experts in the industry and university concerning the influence of these factors were collected by designing a questionnaire and using Likert scaling, and next, decisions were made regarding these indicators. After this process, 51 indicators were approved and categorized into 6 factors and 12 dimensions, based on which the final questionnaire was designed and distributed among 32 managers and experts of MAPNA Group. Using structural equations and Smart PLS software, the research model was fitted and ultimately, surface six indicators were approved for the designed model. Also, the ANP technique was utilized in order to determine the order of factors and the result illustrated that factors namely “propagation“ and “acquisition“ were given the 1st and 2nd order respectively and factor “protection“ was given the last order. According to the conducted researches in the technology management of MAPNA Group, all six facts factors have a significant influence on technology management and hence, all six factors must be paid attention to. In case such a thing does not happen, reaching success in technology management will fail.
Exploring the Mental, Social and Lifestyle Effects of COVID-19 Infection on Syrian Refugees in Jordan: A Qualitative Study
Authors: Kheirallah Khalid A , Ababneh Bayan F , Bendak Heba , Alsuwaidi Ahmed R , Elbarazi Iffat 
Published: December 2021

Abstract
Background: The fear of testing positive for COVID-19 infection has created panic among patients and families and discouraged some people from being tested and receiving medical care. Migrants and refugees are among the vulnerable populations that suffered disproportionately from the COVID-19 crisis. However, their experiences with COVID-19 positivity status have not been investigated. This study aimed to explore the physical, mental, and psychosocial impact of a positive COVID-19 diagnosis on Syrian refugees living in Jordan.Methods: Using a qualitative approach, twenty phone interviews were conducted with ten adult Syrian refugees living within the camp and ten refugees living in non-camp (host-community) settings. A follow-up interviews with five health care providers in the refugee camp were conducted to explore the services and support given to the refugees with COVID-19 infection. The findings were thematically analysed using Braun & Clarke's six-phase framework. Results: Physical effects of a positive COVID-19 status varied according to the seriousness of the condition but affected most participants' lifestyle by adopting more precautionary measures and caring for their physical health. Self-isolation and fear to infect others were common themes identified among all participants living within community settings. Resorting to friends and family members for mental and psychological support was the only option available for all participants. Refugees living within the community preferred to manage their condition at home utilizing traditional remedies and avoided being tested for financial reasons, poor healthcare access and fear of being identified. Refugees living within camps had better access to testing, healthcare, and disease management and did not experience fear of being deported. They did not display worries and feelings of shame and guilt, which were feelings expressed by refugees living within the community. Both groups suffered mental and psychosocial health impacts, as well as social isolation and fear of death and disease complications.Conclusions: COVID-19 infection has negatively impacted refugees’ well-being with noticeable disparities across the different living conditions. While mental health impact seems to be standardized, refugees living within host-community may need more support into managing their condition, accessibility to free testing as well as treatment and healthcare services.
Brain Structural Changes in Regions Within the Salience Network in Patients with Functional Constipation
Authors: Jia Zhenzhen , Li Guanya , Hu Yang , Li Hao , Zhang Wenchao , Wang Jia , Zhang Lei , Tan Zongxin , Lv Shuai , von Deneen Karen M. , Duan Shijun , Cui Guangbin , Zhang Yi , Nie Yongzhan 
Published: December 2021

Abstract
Functional constipation (FCon) is one of the common functional gastrointestinal disorders (FGID). Previous studies reported alterations in cortical morphometry as well as changes in white matter (WM) fiber tracts and thalamo-limbic/parietal structural connectivity (SC). However, whether patients with FCon are implicated in changes in gray matter (GM) volume and associated SC remains unclear. Voxel-based morphometry (VBM) was first employed to examine differences in GM volume between 48 patients with FCon and 52 healthy controls (HC). Diffusion tensor imaging (DTI) with probabilistic tractography analysis was then employed to explore alterations in SC of these regions. Compared with the HC, patients with FCon showed decreased GM volumes in the right middle frontal gyrus (MFG_R), left insula (INS_L), and anterior cingulate cortex (ACC, PFWE < 0.05) which had a negative correlation with abdominal symptoms and difficulty of defecation respectively. Seed-based SC showed patients with FCon had decreased fractional anisotropy (FA) of bilateral INS-ACC, bilateral ACC-MFG, bilateral INS-MFG, increased axial diffusivity (AD) of bilateral ACC-MFG, and decreased radial diffusivity (RD) of bilateral INS-ACC, bilateral ACC-MFG tracts. FA of the right INS-ACC tract had a negative correlation with difficulty of defecation and AD of the ACC-left MFG tract had a negative correlation with stool symptoms. These findings reflect patients with FCon are implicated in changes in GM volumes and corresponding SC in brain regions within the salience network.
Coastal Vulnerability Assessment Based on Multi-Hazardous Events. Case study: Northwestern Coastline of Guinea-Bissau (NC-GB)
Authors: Lopes Namir Domingos Domingos Raimundo , Tianxin Li , Matomela Nametso , Sa Rui Moutinho 
Published: December 2021

Abstract
Guinea-Bissau coastlines are found highly vulnerable to coastal hazards, and this vulnerability will likely increase under future climate changes scenarios. In addition, the multi-hazardous assessment studies have not yet been conducted to clarify the status of vulnerability index to coastal hazards. Therefore, we integrated eight bio-geophysical parameters and elaborate a comprehensive Coastal Vulnerability Index to coastal hazards, stablish the rate of sea-level rise and determine the role of coastal habitats in protecting the shorelines in the Northwestern Coastline of Guinea-Bissau, by using the GIS and Coastal Vulnerability Index of InVEST Model. The study found that, out of 87 km of the studied coastlines, nearly 45 km lie in high to very-high vulnerability index. 17 km are found in a moderate vulnerability index and 25 km are found at low to very-low vulnerability index. The main responsible for high vulnerability registered in Zone-B were the wind and wave exposure, as this coastline is highly exposed to sea. The other reason was the storm surge and sea-level that rises 8.79/year, motivated by low coastal elevation. Mangrove ecosystem that are largely found in Zone-A, play very important role in protecting shoreline from coastal hazards with value 0.61, followed by forest and sand dune that are found mostly in Zone-C with 0.49 and 0.4 respectively, and saltmarsh that are relatively found in Zone-B with value 0.32. These findings can assist coastal managers in cost-effective adaptation plans, provide a scientific basis for sustainable coastal management and guidance for ecological conservation in coastal regions.
The Transcription Factor HcERF4 Confers Salt and Drought Tolerance in Kenaf (Hibiscus Cannabinus L.)
Authors: Yue Jiao , Tang Meiqiong , Zhang Hui , Luo Dengjie , Cao Shan , Hu Yali , Huang Zhen , Wu Qijing , Wu Xia , Pan Jiao , Chen Canni , wang caijin , Chen Peng 
Published: December 2021

Abstract
Ethylene response factors (ERF) are members of the APETALA2/ERF transcription factor family, and they play an important role in plant growth, development, and response to various environmental stresses. In the present study, an ERF transcription factor HcERF4 was isolated and characterized from kenaf. The protein encoded by the HcERF4 has 233 amino acid residues with a theoretical isoelectric point of 8.89 and a predicted molecular weight of 25.53 kDa. HcERF4 had an over than 86.97% identity to HsERF4(XP_039019980.1), and shared a closest phylogenetic relationship with Hibiscus syriacus. Subcellular localization analysis shows that HcERF4 is located in the nucleus. Transactivation assays in yeast demonstrated that HcERF4 functions as a transcriptional activator. The expression of HcERF4 was enriched in leaf and root, and can be induced by salt or drought treatments in kenaf. The VIGS-silenced HcERF4 plant showed significantly reduced plant height, stem diameter, fresh weight, and relative water content (RWC) compared with wild type plants under salt or drought stress condition; In addition, the contents of MDA, O2−, H2O2, and free proline is significantly increased, and the activities of SOD and CAT are significantly reduced. The DAB/NBT staining results showed that the H2O2 and O2− contents in HcERF4-silenced plants were consistent with the determination. Based on these results, it is proposed that HcERF4 plays an important role in regulating salt and drought stress in kenaf.