bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
Authors: Corbacho Jorge , Sanabria-Reinoso Estefanía , Fernández-Miñan Ana , Martínez-Morales Juan R. 
Published: October 2021

AbstractDevelopmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. Here we combined the TRAP approach with adapted enhancer trap methods (trap-TRAP) to systematically generate zebrafish transgenic lines suitable for tissue-specific translatome interrogation. Through the random integration of the eGFP:rpl10a cassette, we have generated stable lines driving expression in a variety of tissues, including the retina, skeletal muscle, lateral line primordia, rhombomeres, or jaws. To increase the range of applications, a UAS:TRAP transgenic line compatible with available Gal4 lines was also generated and tested. The resulting collection of lines and applications constitutes a resource for the zebrafish community in developmental genetics, organ physiology and disease modelling.
Innexin function dictates the spatial relationship between distal somatic cells in the Caenorhabditis elegans gonad without impacting the germline stem cell pool
Authors: Tolkin Theadora , Mohammad Ariz , Starich Todd , Nguyen Ken C. Q. , Hall David H. , Schedl Tim , Hubbard E. Jane Albert , Greenstein David 
Published: October 2021

AbstractGap-junctional signaling mediates myriad cellular interactions in metazoans. Yet, how gap junctions control the positioning of cells in organs is not well understood. Innexins compose gap junctions in invertebrates and affect organ architecture. Here, we investigate the roles of gap-junctions in controlling distal somatic gonad architecture and its relationship to underlying germline stem cells in Caenorhabditis elegans. We show that a reduction of soma–germline gap-junctional activity causes displacement of distal sheath cells (Sh1) towards the distal end of the gonad. We confirm, by live imaging, transmission electron microscopy, and antibody staining, that bare regions—lacking somatic gonadal cell coverage of germ cells—are present between the distal tip cell (DTC) and Sh1, and we show that an innexin fusion protein used in a prior study encodes an antimorphic gap junction subunit that mispositions Sh1. We determine that, contrary to the model put forth in the prior study based on this fusion protein, Sh1 mispositioning does not markedly alter the position of the borders of the stem cell pool nor of the progenitor cell pool. Together, these results demonstrate that gap junctions can control the position of Sh1, but that Sh1 position is neither relevant for GLP-1/Notch signaling nor for the exit of germ cells from the stem cell pool.
Influence of practitioners' characteristics on risk assessment in Green Building projects in emerging economies: a case of Vietnam
Authors: Nguyen Hung Duy , Do Quang Nhat Huu , Macchion Laura 
Published: October 2021

PurposeRisks are considered a significant obstacle to Green Building (GB) development and have recently received significant attention from both construction practitioners and academics. This study aimed to identify critical GB risks and explore the relationship between participants' characteristics and risk assessment.Design/methodology/approachFirstly, a list of GB risks was developed based on a comprehensive literature review and interviewing GB experts. After that, a survey of 207 construction professionals was then conducted to validate these GB risk factors. Finally, this research adopted the ANOVA test and hierarchical regression analysis to examine the relationship between participants' characteristics and risk assessment.FindingsThe results provided a list of GB risks classified and evaluated according to the GB project life cycle and, thus, may serve as a helpful reference for GB practitioners. Notably, the ANOVA analysis revealed that risk assessment negatively correlates with participants' GB experience, while their industry experience does not affect risk assessment. Furthermore, the hierarchical regression analysis proved that participant roles do not moderate the association between risk assessment and GB experience.Originality/valueThis study contributed to GB literature by implementing empirical research on GB risks in a developing country. The results implied the essential role of professionals with rich GB experience in risk management in GB projects. Furthermore, this research could help construction practitioners understand GB risks adequately and thereby have better risk-management strategies for future GB projects.
Becoming cynical and depersonalized: how incivility, co-worker support and service rules affect employee job performance
Authors: Baker Melissa A. , Kim Kawon 
Published: October 2021

Purpose
Customer incivility is commonplace across service industries. Yet, there is little that is known about how uncivil customers affect employees. The purpose of this study is to examine how uncivil customer interactions affect employees’ cynicism, depersonalization and job performance.

Design/methodology/approach
Study 1 uses the qualitative critical incident technique to content analyze employee perceptions of customer incivility and how it affects their job performance. Study 2 uses a 2 (incivility frequency: high vs low) × 2 (co-worker support: high vs low) × 2 (service rule commitment: high vs low) quasi-experimental between-subjects design.

Findings
Results find that there is a significant interaction effect of customer incivility frequency, co-worker emotional support and service rule commitment on employee cynicism and depersonalization, which leads to decreased job performance and more harmful experiences to other customers.

Practical implications
The findings provide practical implications on the importance of managing customer incivility, providing co-worker support and how this affects employee attitudes and service they deliver to other customers.

Originality/value
The results build upon the incivility, co-worker support and service rule commitment literature, conservation of resources theory, as well as identifying key variables core to hospitality and tourism research: cynicism and depersonalization that provide important implications for actions of tourism and hospitality firms.
The origin and evolution of the concept of servitization: a co-word and network analysis
Authors: Pinillos María‐José , Díaz-Garrido Eloísa , Martín-Peña María-Luz 
Published: October 2021

Purpose
The purpose of this paper is to analyze the origins and evolution of the concept of servitization by studying the definitions of servitization provided in the literature. Servitization represents an academic field that has grown rapidly since its inception. However, the conceptualization of servitization varies greatly, in part because of the number of studies on this topic and the fact that it has been analyzed in a range of disciplines using a number of theoretical approaches. There is a need to standardize the vocabulary to create a general definition that can support the development of theory in this domain and help legitimize servitization as a research area.

Design/methodology/approach
This study conducts a systematic, quantitative analysis of a broad set of definitions of servitization. Specifically, this study performs content analysis (combining co-word analysis and social network analysis) and consensus analysis. This study develops a strategic diagram to represent the morphology of the research network.

Findings
The definitions of servitization are deconstructed and analyzed in depth to create a comprehensive picture of the research on this topic. This analysis reveals the origins and evolution of this research area. The results show a low degree of consensus among scholars regarding the concept of servitization. This study proposes a definition that should be widely accepted thanks to its inclusion of the core terms from other definitions. Explicit recognition of multiple approaches to defining the term can help practitioners and researchers. Predictions about future progress in this area are discussed.

Originality/value
A universal definition of servitization is proposed based on the results of co-word and network analysis. This definition unifies a range of multidisciplinary viewpoints. From a practical perspective, the key vocabulary in servitization research is highlighted.
Serum zinc levels in patients with phlegmons as a marker of the severity of infection and the potential of zinc supplementation to reduce the risk of recurrence
Authors: Iijima Shigeruko , Takayama Noriko 
Published: October 2021

AbstractWe analyzed the serum zinc levels of 37 patients with 40 phlegmon lesions. The mean serum zinc level was 52.1 ± 16.4 μg/dL. The serum zinc level was negatively correlated with the C‐reactive protein (CRP) level (r = −0.66) and white blood cell (WBC) count (r = −0.56). It was also positively correlated with the serum levels of albumin, hemoglobin, and hematocrit (r = 0.57, 0.50, and 0.50, respectively). Patients with serum zinc levels of <60 μg/dL had higher CRP levels and WBC counts (p < 0.005 and p < 0.05, respectively) and lower albumin, hemoglobin, and hematocrit levels (p < 0.001, p < 0.01, and p < 0.01, respectively), and were more likely to be hospitalized (p < 0.05) than those with serum zinc levels of ≥60 μg/dL. Patients with low serum zinc levels were given zinc tablets. Three of the seven patients who developed recurrent phlegmons did not develop any further lesions after taking zinc tablets for >10 months. Of the remaining patients, one only developed a minimal lesion, and another two experienced recurrence twice but did not have any further lesions for 10 and 15 months, respectively. These findings indicate that in patients with phlegmons the serum zinc level is a suitable marker of the severity of infection, and zinc supplementation reduces the risk of further recurrence in patients whose lesions relapse.
Social character, interdependence, and the dualities of other‐directedness
Authors: Shilling Chris , Mellor Philip A. 
Published: October 2021

AbstractDavid Riesman's exploration of the other‐directed characterological form, suited to corporate capitalism and the rise of the service sector, became one of the most influential sociological analyses of the twentieth century. Yet sociologists interested in the contemporary fate of those dispositional qualities suited to mutual adjustment confront a paradox: why, in an age of increasing interdependencies apparently conducive to the sustenance of other‐directedness, are we witnessing rising concerns about the resurgence of social sectarianism? Most accounts of this tension rely upon structuralist explanations of late modernity's disruptive impact, or psychologistic accounts of group allegiance. In contrast, we develop a meso‐level analysis that highlights an increasingly consequential duality at the heart of other‐directedness itself: the qualities associated with this characterological form still facilitate selective forms of mutuality, but the demands it places upon people in the current era have also prompted growing levels of resentment and antagonism.
Learning and application of intracorporal slipping knot techniques in minimally invasive surgery
Authors: Romero Philipp , Carstensen Leonie , Kössler‐Ebs Julia , Wennberg Erica , Müller‐Stich Beat P. , Nickel Felix , Günther Patrick 
Published: October 2021

AbstractAimIntracorporal knot tying (ICKT) and suturing in minimally invasive surgery (MIS) are key skills for advanced procedures. The best choice for an intracorporal slipping knot tying technique had not been defined. The aim of this study was to compare two intracorporal slipping knot techniques: the classical C‐loop technique (Variant I) and the square‐to‐slip technique without changing leadership hand (Variant II).MethodsA laparoscopic box trainer including laparoscope was used for ICKT. A total of 120 slipping knots were evaluated in two groups with different levels of surgical education. The first group (n = 5) consisted of senior surgical physicians proficient in laparoscopic surgery. The second group (n = 10) comprised medical students without any prior experience in laparoscopic surgery. The medical student group received a 1‐hour hands‐on training session. Participants were assessed using the Global Rating Scale of the Objective Structured Assessment of Technical Skills, procedural implementation, knot quality, and task time.ResultsIn the medical student group, performance in all parameters was greater for Variant I compared with Variant II (P < .005). Contrary to the students group, senior physicians demonstrated significantly faster task time in Variant II compared with Variant I (P = .001). No significant differences were observed in the remaining parameters.ConclusionFor novices, the intracorporal slipping knot is easier to learn with the C‐loop technique (Variant I) than with the square‐to‐slip technique (Variant II). For surgeons experienced in MIS, the square‐to‐slip technique without changing leadership hand (Variant II) was superior to the slipping knot in the C‐loop technique (Variant I) only by time savings. The knot quality, as the most important variable, showed equally good results between both ICKT techniques.
A chromosome‐scale genome assembly and annotation of the spring orchid (Cymbidium goeringii)
Authors: Chung Oksung , Kim Jungeun , Bolser Dan , Kim Hak‐Min , Jun Je Hoon , Choi Jae‐Pil , Jang Hyun‐Do , Cho Yun Sung , Bhak Jong , Kwak Myounghai 
Published: October 2021

AbstractCymbidium goeringii, commonly known as the spring orchid, has long been favoured for horticultural purposes in Asian countries. It is a popular orchid with much demand for improvement and development for its valuable varieties. Until now, its reference genome has not been published despite its popularity and conservation efforts. Here, we report the de novo assembly of the C. goeringii genome, which is the largest among the orchids published to date, using a strategy that combines short‐ and long‐read sequencing and chromosome conformation capture (Hi‐C) information. The total length of all scaffolds is 3.99 Gb, with an N50 scaffold size of 178.2 Mb. A total of 29,556 protein‐coding genes were annotated and 3.55 Gb (88.87% of genome) repetitive sequences were identified. We constructed pseudomolecular chromosomes using Hi‐C, incorporating 89.4% of the scaffolds in 20 chromosomes. We identified 220 expanded and 106 contracted genes families in C. goeringii after divergence from its close relative. We also identified new gene families, resistance gene analogues and changes within the MADS‐box genes, which control a diverse set of developmental processes during orchid evolution. Our high quality chromosomal‐level assembly of C. goeringii can provide a platform for elucidating the genomic evolution of orchids, mining functional genes for agronomic traits and for developing molecular markers for accelerated breeding as well as accelerating conservation efforts.
Withdrawal from cocaine conditioning progressively alters AMPA receptor‐mediated transmission in the ventral hippocampus
Authors: Preston Collin J. , Wagner John J. 
Published: October 2021

AbstractDrugs of abuse, such as cocaine, produce aberrant changes in synaptic transmission and plasticity that emerge throughout withdrawal. One region of the brain that displays a high degree of synaptic plasticity, as well as connectivity with mesolimbic structures such as the nucleus accumbens, is the ventral hippocampus (vH). Here, we investigated the effects of an escalating cocaine dosing schedule on vH CA1 excitatory transmission by measuring place preference and recording excitatory postsynaptic currents (EPSCs) at three different withdrawal time points: withdrawal day (WD) 2, 9 or 28. Behaviourally, this escalating cocaine‐conditioning protocol was capable of producing conditioned place preference that persisted through WD28. Physiologically, cocaine conditioning produced an increase in vH excitatory transmission on WD2 that appeared to be the result of an increase in calcium‐impermeable (CI)‐AMPA receptor density. Excitatory transmission was still enhanced in cocaine‐treated animals on WD9; however, a significant increase in the contribution of calcium‐permeable (CP)‐AMPA receptors to EPSCs was detected as compared with WD2. By WD28, these CP‐AMPA receptors provided a major contribution to vH CA1 excitatory transmission, resulting in synaptic responses distinct from WD2 and WD9. Taken together, these results highlight progressive changes in vH synaptic transmission during withdrawal that may enhance cocaine contextual associations.
The Quadratic‐Exponential‐Pubertal‐Stop model is valid for analysing human growth patterns and developing novel growth references
Authors: Holmgren Anton 
Published: October 2021

AbstractAimHuman growth patterns are important, especially in paediatrics and public health, and the aim of this review was to provide an overview of human growth, especially secular changes of growth and pubertal growth.MethodsThis review of human growth was mainly based on studies published during the 20th and early 21st centuries. Special attention was paid to secular changes, pubertal growth, Nordic growth studies and the contribution of the Quadratic‐Exponential‐Pubertal‐Stop (QEPS) growth model for analysing growth patterns.ResultsHuman growth patterns showed wide variations between different individuals, sexes and populations and over time. There were ongoing positive secular change in height in four of the Nordic countries, Denmark, Finland, Norway and Sweden, over four decades. Childhood weight status had linear correlations with specific pubertal growth, in both healthy children and those with severe obesity. The QEPS model provided novel estimates of pubertal growth that made it possible to conduct more detailed analyses of pubertal growth than before. Growth references, adjusted for puberty, have been developed, and future opportunities for using the QEPS model for growth studies are highlighted.ConclusionThe QEPS was a valid growth model for analysing human growth patterns and developing novel types of growth references.
Evaluating prediction methods for glomerular filtration to optimise drug doses in obese and nonobese patients
Authors: Busse David , Borghardt Jens Markus , Petroff David , Pevzner Alice , Dorn Christoph , El‐Najjar Nahed , Huisinga Wilhelm , Wrigge Hermann , Simon Philipp , Kloft Charlotte 
Published: October 2021

Aims: The most suitable method for predicting the glomerular filtration rate (GFR) in obesity is currently debated. Therefore, multiple GFR/creatinine clearance prediction methods were applied to (morbidly) obese and nonobese patients ranging from moderate renal impairment to glomerular hyperfiltration and their predictions were rated based on observed fosfomycin pharmacokinetics, as this model drug is exclusively eliminated via glomerular filtration.Methods: The GFR/creatinine clearance predictions via the Chronic Kidney Disease Epidemiology Collaboration (CKD‐EPI), Modification of Diet in Renal Disease (MDRD; indexed and de‐indexed by body surface area) and creatinine clearance via the Cockcroft–Gault formula (CLCRCG) using different body size descriptors were compared to the fosfomycin clearance (CLFOF) from 30 surgical patients (body mass index = 20.1–52.0 kg m−2), receiving 8000 mg as intravenous infusion.Results: The concordance between CLFOF and creatinine clearance predictions was highest for CLCRCG employing either ideal body weight or adjusted body weight (if body mass >1.3 ideal body weight; CLCRCG_ABW‐Schwartz, concordance‐correlation coefficient [95% confidence interval] = 0.474 [0.156; 0.703], CCC) and GFR predictions via the de‐indexed MDRD equation (concordance‐correlation coefficient = 0.452 [0.137; 0.685]). The proportion of predicted GFR values within ±30% of the observed CLFOF (P30 = 72.3–76.7%) was only marginally lower than the reported P30 in the original CKD‐EPI and MDRD publications (P30 = 84.1–90.0%).Conclusion: This analysis represents a successful proof‐of‐concept for evaluating GFR/creatinine clearance prediction methods: Across all body mass index classes CLCRCG_ABW‐Schwartz or the de‐indexed MDRD were most suitable for predicting creatinine clearance/GFR also in (morbidly) obese, CKD stage <3B individuals in therapeutic use. Their application is proposed in optimising doses for vital therapies in obese patients requiring monitoring of renal function (e.g. methotrexate dosing).
Outfoxing the fox: Effect of prey odor on fox behavior in a pastoral landscape
Authors: Andrewartha Tim A. , Evans Maldwyn J. , Batson William G. , Manning Adrian D. , Price Catherine , Gordon Iain J. , Barton Philip S. 
Published: October 2021

AbstractInvasive mammalian predators have had a devastating effect on native species globally. The European red fox (Vulpes vulpes) is one such species where it has been introduced in Australia. A novel but unexplored tactic to reduce the impact of mammalian predators is the use of unrewarded prey odors to undermine the effectiveness of olfactory hunting behavior. To test the viability of unrewarded prey odors in an applied setting we investigated how foxes responded to the odors of three different prey species. We used the odors of two locally extinct native Australian marsupials; the eastern quoll (a smaller carnivore) and eastern bettong (a fungivore), and the European rabbit, an introduced herbivore. Conducting our research over a period of 3 weeks in a pastoral environment in South‐eastern Australia, we used video observations of foxes' behaviors, as they encountered the different odors. We found a reduction in the number of fox visits to bettong odors in the third week. In contrast, we observed a sustained number of visits to rabbit odors. Foxes also spent more time investigating rabbit odors and displayed longer durations of vigilance behavior at quoll odors. Our results support the hypothesis that the exposure of wild foxes to unrewarded odors of novel prey species can reduce their interest in these odors, which might translate to a reduction in predation pressure. Our results also suggest, however, that olfactory pre‐exposure may not be as effective at reducing fox interest in a competitor species' odor.