bluuebunny/crossref_metadata_2025_split huggingface dataset:
523 Late-Breaking: Non-nutrient Factors in Colostrum: Systems Biology of Mammary Derived Bioactive Proteins and Gut Receptors That Network Functions in the Bovine Neonate
Authors: Schalich Kasey , Koganti Prasanthi , Nguyen Blake , Reiff Olivia , Lamb Cassandra , Gurung Shailesh , Selvaraj Vimal 
Published: October 2021

Abstract
Maternal-offspring coevolution has introduced the biological concept of “neonatal programming,” in which soluble proteins of varying abundance in bovine colostrum can have targeted activities in the calf gut. Still, the identities and developmental programming mechanisms of the full profile of colostrum proteins on transiently expressed gut receptors/transporters, as well as the ultimate functional responses in the calf, remain to be completely elucidated. The objective of this study was to address this gap in knowledge using systems biology. First, we biopsied the mammary gland and examined the transcriptome in primiparous and multiparous Holstein cows (at parturition/day 0 contrasted to 40–50 days after parturition; n = 4–5/group; FDR&lt; 0.05) and used a bioinformatics algorithm to delineate transcripts coding proteins destined to be secreted into colostrum. In parallel, we analyzed the neonatal small intestine (0 day-old contrasted to 7 day-old; n = 3–5/group; FDR&lt; 0.05) to identify transcripts that code for membrane receptors/transporters precise to the period of colostrum consumption. Integrative analysis of these results highlighted 44 possible signaling circuits (cutoff: &gt;10 nCPM) directed by colostrum in the neonatal gut, providing a consolidated predictive understanding of colostrum-mediated effects that might occur in the neonate during this crucial period in development. These findings also represent the first mechanistic insight into mammary-sourced components that target the neonatal gut to regulate aspects of postnatal development that encompass intestinal maturation, gut-based secondary signaling, and establishment of the gut microbiome, all relevant to long-term health and development. Towards applications, these results are poised to offer novel opportunities to enhance commercial supplements via biomimicry to better reflect the physiology supporting neonatal growth and development.
518 Late-Breaking: Effect of Feedlot Diets on Environmental Emissions from Four Representative U.S. Feedlots and the Beef Production System
Authors: Gilreath Jessica , Rotz Al , Place Sara , Thoma Greg , Wickersham Tryon 
Published: October 2021

Abstract
Our objective was to evaluate effects of feedlot dietary management strategies on environmental impacts and net returns of feedlot operations in the United States. Representative feedlots were simulated with the Integrated Farm System Model (IFSM 4.6; USDA-ARS, University Park, PA) to quantify baseline environmental impacts of feedlot production and full US beef cattle production systems. The simulated dietary strategies included: 10% increase in feed efficiency, use of less water intensive forages, 10% increase in byproduct inclusion, 10% improvement in water use efficiency of corn, and steam-flaking of corn. Days on feed and head finished per year were held constant for all strategies to have equal comparisons to baseline results. Dietary management strategies were individually modeled and simulated in IFSM for each feedlot operation to obtain intensities (expressed per kg gain) for greenhouse gas (GHG) emissions, fossil energy use, blue water consumption, and reactive nitrogen loss. Feedlot operations were then linked with cow-calf, stocker, and backgrounding operations to estimate environmental intensities (expressed per kg CW) for full cattle production systems. Improving feed efficiency had the greatest effect on reducing carbon emission intensities (6%), energy use intensity (8%), blue water use intensity (9%), and reactive N loss intensity (4%) for feedlot operations. Increasing corn byproduct inclusion resulted in 9% reduction in blue water use intensity. However, byproduct inclusion increased reactive N loss intensity by 11% as a result of greater protein concentrations in the diet. Switching from rolled corn to steam flaked corn increased energy use intensity by 9%, but little to no changes (1% increase to 3% reduction) were observed for other environmental intensities. Improved feed efficiency was the most effective strategy to reduce environmental footprints of beef cattle production (1 to 2% reductions). Overall, feedlot dietary strategies were less pronounced for the full beef production system compared with feedlot results.
288 Inclusion Effects of Quebracho (Schinopsis Balansaie) Extract and Active Dry Yeast (Saccharomyces Cerevisiae) in Beef Cattle Limit-fed a Grower Ration
Authors: Rivera Madeline E , Batista Luiz Fernando Dias , Norris Aaron B , D’Souza Genevieve M , Tedeschi Luis O 
Published: October 2021

Abstract
Plant secondary metabolites (PSM) and yeast supplemented in growing cattle have been reported as improving dry matter digestibility (DMD) and reducing enteric methane emissions. This study aimed to evaluate the effect of combined supplementation with condensed tannin (CT; Schinopsis balansae) extract and active dry yeast (ADY; Saccharomyces cerevisiae) on fermentation dynamics, utilizing in vitro gas production (IVGP) technique. A 2 × 2 Latin square design was used to study fermentation patterns of four dietary treatments (CON- no CT and no ADY, ADY alone, CT alone or combined CT + ADY). Animals received daily CT at 1% DM and 10 g of ADY, respectively. On d 0, 7, 14, 21, 28, and 35 rumen inoculum was collected from 23 fistulated steers (284.3 ± 4.1 kg) four hours post-feeding. Samples were incubated under anaerobic conditions at 39oC for 48 h with 200 mg of a grower diet (14.8% CP, 40.6% NDF, 88.5% DM). Gas parameters were analyzed using a mixed linear statistical model. There was a day effect for total gas production (TGP; P &lt; 0.001), non-fiber carbohydrate degradation (P = 0.031) and fractional degradation assuming an asymptote model (P = 0.015). Both asymptote and non-fiber fractional degradation rate estimates had an interaction between Day × TRT (P = 0.001 and 0.0104, respectively). Data were analyzed using polynomial contrasts showed a difference in non-fiber fractional rate of fermentation for CON × CT and CT × ADY (P = 0.052 and 0.054, respectively). This was also true if an asymptote model was assumed (P = 0.0 34 and 0.034, respectively). We concluded that combined supplementation of CT and ADY exhibited similar IVGP trends over time, this may be because animals only received a grower diet at 1.5% shrunk BW. Future studies should investigate the impact of combined supplementation on varying levels of concentrate diets.
286 Effects of Supplementation of Quebracho Extract Supplementation on Ruminal Ph of Growing Beef Steers
Authors: Batista Luiz Fernando Dias , Norris Aaron B , Adams Jordan , Tedeschi Luis O 
Published: October 2021

Abstract
Rumen acidosis is a common metabolic disorder occurring when organic acid production exceeds clearance capacity, reducing ruminal pH. Acidosis occurrence has been directly correlated to the ratio of concentrate to forage in the diet. However, the rates of substrate fermentation and acid absorption vary at different locations in the rumen. The objective of this study was to determine the pH in different locations of the rumen using 16 rumenally- cannulated steers (309 ± 43 kg) receiving quebracho extract (QT; Schinopsis balansae) within a grower-type diet [25:75 forage-to-concentrate, dry matter (DM) % basis]. Animals were randomly assigned to one of four dietary treatments (n = 4): QT at 0, 1, 2, and 3% of DM (QT0, QT1, QT2, and QT3). Animals were adapted to the basal diet (QT0) for 12-d before being introduced to predetermined treatments for four weeks, with feed provided twice daily to allow ad libitum intake. Weekly measurements of ruminal fluid pH and redox potential (Eh) were taken four h post-feeding using a portable pH and redox meter probe in four locations of the rumen (cranial sac, ventral sac, dorsal sac, and reticulum). Data were analyzed using a random coefficients model with the pen as a random effect and week as repeated measures. The DM intake was included as a covariate. There was no interaction among diet, location, and week (P ≥ 0.925) on pH. Overall, ruminal pH was lower for QT0 and QT1 compared to QT3 (P &lt; 0.001). Ruminal pH in the cranial sac and reticulum was greater than in the dorsal sac (5.98, 6.03, and 5.87, respectively; P = 0.001). Redox potential was lower for QT0 in week 1 than all other treatments (P = 0.042). This study indicated that pH differs among locations of the rumen regardless of QT supplementation level and days on feed.
293 Effect of Supplementing Rumen-protected Methionine on Blood Parameters and Ruminal Metabolites in Lactating Holstein Dairy Cows
Authors: Wei Jialin , Cao Zhijun , Li Yuanxiao 
Published: October 2021

Abstract
The aim of the present study was to evaluate the effects of reducing dietary crude protein (CP) levels and supplementing rumen-protected methionine (RPM) on blood parameters and ruminal metabolites in lactating Holstein dairy cows. A total of 30 lactating Holstein dairy cows (60 ± 7 d in milk; mean ± SD) were randomly assigned to 1 of 2 treatments: diet containing 17.3% CP without RPM (control group; CON); diet containing 16.4% CP with supplementing 15.0 g/d of RPM (treatment group; RPM). All repeated, continuous data were subjected to PROC MIXED procedure of SAS (SAS version 9.2, SAS Institute Inc., Cary, NC.). The results related to blood showed that cows in RPM group exhibited lower concentration of blood urea nitrogen than that in CON group (P &lt; 0.001). Moreover, there were no differences among treatments on concentrations of aspartate transaminase, alanine transaminase, alkaline phosphatase, globulin and albumin (P &gt; 0.05). In ruminal metabolites, microbial CP (MCP) of dairy cows in RPM group was higher compared with CON group (P = 0.006). The concentrations of butyrate, valerate and isovalerate of RPM group were higher than that of CON group at 2h after feeding (P &lt; 0.05). In conclusion, lower dietary CP with RPM supplementation could improve nitrogen utilization of dairy cows and synthesis of MCP in rumen, as well as change volatile fatty acids production at 2h after feeding.
305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet
Authors: Osorio Andrea M , Tonelli Nardi Kaue T , Fávero Igor Gomes , Scaranto Silva Kaliu G , Coello Kymberly D , Raudales Angel A , Ciriaco Francine M , Sarturi Jhones O Onorino , Henry Darren D 
Published: October 2021

Abstract
The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.
311 Effects of Interactive Activity at the Feedbunk on Performance, Feed Efficiency and Feeding Patterns in Feedlot Cattle
Authors: O’Reilly Keara , Carstens Gordon , Wottlin Lauren , Johnson Jocelyn R R , Daigle Courtney L 
Published: October 2021

Abstract
The objective of this study was to determine if the magnitude of interactive feeding activity at the feedbunk affects feed efficiency and feeding behavior patterns in beef cattle. This study used data from 498 crossbred steers (3 trials) fed high-concentrate diets in pens with electronic feed bunks (GrowSafe Systems). Timestamp data for bunk visit (BV) events were used to develop an algorithm to quantify those BV events deemed to be replacement events; Defined as those that occur when an animal displaces and replaces another animal from the feedbunk within a specified period of time (replacement criterion). For this study, a replacement criterion of ≤ 18 s was used. A replacement activity index (RAI) was computed for each steer as the number of replacement events divided by total BV events. Within pen, steers were classified into 1 of 3 phenotypes based on ± 0.5 SD from mean RAI. Steers with high RAI (&gt; 0.5 SD from mean RAI) were hypothesized to be more interactive at the feedbunk, whereas steers with low RAI were less interactive at the feedbunk. A mixed model with RAI class as fixed effect, and trial and pen within trial as random effects was used for data analysis. The low RAI steers had 6% fewer BV events that were 12% longer (P &lt; 0.001) in duration than high RAI steers. Additionally, eating rate (g/min) was 14% slower (P &lt; 0.001) and time to approach the feedbunk following feed delivery 6 min longer (P &lt; 0.001) in low vs high RAI steers. The low-RAI steers had substantially less (P &lt; 0.01) feeding activity during peak feeding time than high RAI. Further research is warranted to evaluate interactive feeding activity at the feedbunk as a potential indicator of performance and feed efficiency in feedlot cattle.
310 Growth Performance and Carcass Characteristics of Beef Steers Consuming Finishing Diets with an Added Nutritional Packet
Authors: Tonelli Nardi Kaue T , Onorino Sarturi Jhones O , Henry Darren D , Ciriaco Francine M , Huerta-Leidenz Nelson O , Osorio Andrea M , Fávero Igor Gomes , Scaranto Silva Kaliu G , Rush Camron J 
Published: October 2021

Abstract
The effects of a nutritional packet fed to beef steers during the final 64 d of the feedlot finishing phase on growth performance and carcass characteristics were evaluated. Crossbred-Angus steers (n = 120; initial BW = 544 ± 52 kg) were assigned to 30 pens (4 steers/pen; 15 pens/treatment) in a randomized complete block design where pen represented the experimental unit. A steam-flaked corn-based finishing diet was fed ad libitum, and treatments were applied as follows: 1) control and 2) 30 g/steer-daily (DM-basis) of the nutritional packet (containing live yeast [8.7 Log CFU of Saccharomyces cerevisiae], vitamin C [5.4 g/kg of Ascorbic acid], vitamin B1 [13.33 g/kg of Thiamine hydrochloride], sodium chloride [80 g/kg] and potassium chloride [80 g/kg]). The nutritional packet used ground corn as a carrier and was included at 1 % of diet DM. Orts were quantified daily (if any) and subtracted from the total dietary DM offered to calculate DM intake. Unshrunk BW were collected on d 0, 21, 42 and 64 before daily feeding at 0630 h. Data were analyzed using the GLIMMIX procedure of SAS. Average daily gain (P = 0.89), dry matter intake (P = 0.57) and, gain:feed (P = 0.66) were not affected by the inclusion of the nutritional packet. The 12th rib fat deposition was increased (P ≤ 0.02) for cattle fed the nutritional packet compared with control (15.70 vs. 13.36 mm, respectively). Consequently, calculated yield grade was also increased (P ≤ 0.03) for cattle consuming the nutritional packet (3.32 vs. 3.02). An improved carcass fat-deposition without any deleterious effects on growth performance potentially warrant less days on feed when cattle receive such nutritional packet.
314 Carbohydrases Enzymes Improve the Productive Performance of Grazing Nellore Bulls Fed Different Nutritional Plans During the Rainy Season
Authors: Acosta Anderson , Perdigão Alexandre , Vasconcellos Guilherme S , de Carvalho Victor Valério , Acedo Tiago S , Pedrini Cibele , Gandra Jefferson 
Published: October 2021

Abstract
The aim with this trial was to evaluate two nutritional plans with the addition of dosages of carbohydrases enzyme blend on productive performance of grazing Nellore bulls in rainy season. One hundred and two contemporary Nellore steers (BW = 294.10 ± 3.35 kg) were equally distributed in six paddocks (17 steers/paddock) following a completely randomized design in a 2x3 factorial arrangement, being the factors two nutritional plans: mineral-proteic supplementation (Fosbovi® Proteico 30; PS) offered at 0,1% BW or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW); and three levels of enzymatic carbohydrases blend (0, 4,75 or 9,50 g/animal/day). The enzymatic blend was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX); Both enzymes and supplemented were provided by DSM Nutritional Products Brasil S.A. Supplement intake and animal weighting were carried out daily and individually by an automatic scale system installed in each paddock. Data were analyzed using PROC MIXED of SAS and means were compared by polynomial regression. Animals that received PES had greater ADG (0.896 vs. 0.775 kg/day; P &lt; 0.001) compared to those fed PS. Intermediary level (4.75 g/day) of carbohydrate enzymes also presented the highest ADG in PES compared to 0.0 level (0.941 vs. 0.896 kg/day; P = 0.006). Still, higher enzyme levels (9.5 g/day) presented greater ADG on PS, when compared to 0.0 level (0.852 vs. 0.775 kg/day, P = 0.006). PES animals presented greater intake, compared to PS (1.112 vs. 0.432 kg/day, P &lt; 0.001). According to polynomial regression, it is recommended to include 4.75 g/day of these carbohydrases enzymes in PS and 4.39 g/day in PES for growing Nellore bulls in rainy season.
319 Predicting Urinary Nitrogen Excretion from Milk Urea Nitrogen of Fresh Forage or Total Mixed Ration Fed Dairy Cows: A Meta-analysis
Authors: Beck Matthew R , Marshall Cameron , Garrett Konagh , Foote Andrew P , Vibart Ronaldo , Pacheco David , Gregorini Pablo 
Published: October 2021

Abstract
Urine nitrogen excretion (g/d; UN) represent a significant environmental impact for both confinement feeding and pastoral based dairy systems. It is difficult to measure UN directly due to animal handling and labor requirements, especially in forage based production systems. The currently available milk urea nitrogen (MUN) equations have been shown to overestimate UN excretion of grazing dairy cows compared with an equation using urinary creatinine and UN concentration, indicating that diet may alter the relationship between MUN and UN. This potential was explored using data retrieved (treatment means: n = 69 and 27 for fresh forage [FF] and total mixed ration [TMR] fed cattle, respectively) from the literature and new data obtained from dairy cows fed FF (n = 15) in metabolism crates was used to test the new equations. The TMR data from literature was further split into a training set (to develop the model; n = 53) and a test set (to validate the model; n = 16). There was an interaction for diet type (P &lt; 0.01) where UN (g/d) = 0.023 × MUN (mg/dL) × live-weight (kg, LW) for TMR fed cattle, (similar to a pre-established equation); however, UN (g/d) = 0.015 × MUN × LW for FF fed cattle. For FF based equations, the New MUN equation and the creatinine equation showed good precision and accuracy (Lin’s CCC = 0.79 and 0.74, respectively) and adequate predictive ability (RMSEP = 29.8 and 35.9, respectively). The new MUN equation for TMR fed cattle showed excellent accuracy and precision (Lin’s CCC = 0.87) with good predictive ability (RMSEP = 24.3) for UN excretion (observed mean = 216.5 g/d). The new equations generated during this meta-analysis provide promising predictive ability of UN excretion, which can be used for management considerations, future research, and policy making.
322 Impact of Feed Restriction and Fasting Suffered in Assembly Centers and Transportation on Energy Balance and Gut Permeability in Unweaned Angus-holstein Calves
Authors: Pisoni Lucía , Marti Sonia , Blanch Marta , Pastor Jose J , Devant Maria 
Published: October 2021

Abstract
Twenty unweaned Angus-Holstein calves (44.1 ± 2.0 kg; 14.7 ± 0.63 d) were used to evaluate the effects of feed restriction and fasting on energy balance (serum concentration of glucose, BHBA, and NEFA), and intestinal permeability (serum concentration of citrulline, Cr-EDTA, lactulose, and D-mannitol). Calves were randomly assigned to 1 of 4 treatments that simulated assembly center management and transportation; Control (CT; n = 5): from d-4 to -1, 2.5 L of milk replacer (MR) twice daily, concentrate and straw ad-libitum; Mild (MD; n = 5): only MR (d-4 to -1) as described for CT, and on d-1 feed withdrawal for 9 h; Moderate (MO; n = 5): only MR as described for CT and on d-1 feed withdrawal for 19 h; and Severe (SV; n = 5): only 2.5 L of a hydrate solution (HS) twice daily (d -4 to -1) on d-1 feed withdrawal for 19 h. From d 0 to day 7 all calves were fed MR, concentrate, and straw ad-libitum. Data were analyzed using mixed models with repeated measures. At d -1 for SV and d 0 in all restricted calves (MD, MO, SV), serum glucose concentration was lesser (P &lt; 0.01) compared with CT. At d 0 NEFA and BHBA serum concentrations increased (P &lt; 0.01) in SV calves compared with the other treatments. Serum citrulline concentration (P &lt; 0.05) was lower for SV on d -1 and greater for CT on d 2 compared with other treatments. CT calves had the lesser concentration of Cr-EDTA (d -1 and d 0, P =0.04), lactulose (d 0, P =0.02), and D-mannitol (d 0, P =0.01) compared with MD, MO, and SV. The degree of dietary restriction, the type of liquid diet, and amount of fasting hours affected calves’ serum concentration of markers indicative of energy balance and gut permeability.