bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Temperature-induced Spindle Destabilization Results in Aborted Pollen Production in Populus
Authors: Li Zhiqun , Zhao Yifan , Cheng Xuetong , Kong Bo , Sang Yaru , Zhou Qing , Wu Jian , Zhang Pingdong 
Published: October 2021

Abstract
High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in Populus canescens. However, the mechanism by which high temperature induces pollen abortion remains unknown. Here, pollen abortion was induced by exposing male flower buds of P. canescens to 38 and 41 °C; pollen morphology, meiotic abnormalities, defects of the meiotic microtubular cytoskeleton, and tapetum development were characterized, and expression analysis of the Actin gene was conducted. We found that the dominant meiotic stage, temperature, and duration of treatment significantly affected the percentage of high temperature-induced aborted pollen. Damaged spindle microtubules and depolymerized microtubular cytoskeletons were observed, which resulted in many lagging chromosomes at anaphase Ⅰ and Ⅱ, as well as aneuploid male gametes and micronuclei, generating aborted pollen grains. Tapetum disintegration was also delayed. However, the anther dehisced normally, and some viable pollen grains were released, suggesting that the delayed degradation of the tapetum was not responsible for pollen abortion. A significant reduction in PtActin gene expression was detected in treated cells, indicating that spindle actin was disrupted. The spindle actin appeared to protect cells against chromosome segregation errors during meiosis.
Application of Artificial Dermal Graft Combined with Fascia Sleeve Flap in the Treatment of Chronic Bone and Steel Plate Exposed Wounds of Lower Extremities
Authors: Li Yong , Zhang Zhi-bo , Liu Ji-song , Wu Zhu-min , Sun Xin-cheng , Zhao Yu-tin , Zhang Xiang-zhou 
Published: October 2021

Abstract
Background:Bone tendon or graft exposure such as steel plate is common after severe trauma of lower extremity.The traditional repair method is to use a variety of skin flaps to cover the exposure, but the wound can not heal after operation, or the wound dehiscence, ulcer, sinus, etc. occur again after short healing, and the bone plate is exposed again.The reason for this result is that the space around the bone plate is not well closed when the flap is covered，dead space is formed, blood and exudate accumulate, hematoma forms or infection occurs, and finally the wound breaks.Also due to swelling and contracture after flap operation,the tension of the suture between the flap and the receiving area is too large and becomes thin and cracked, forming an exposed state.In order to solve the above problems, we have carried out the research on "Application of artificial gradient combined with fascia sleep flap in the treatment of chronic bone and steel plate exposed woods of lower extremes".Methods:In this paper, 11 cases of chronic wounds with bone exposure and skin necrosis after steel plate implantation were selected. First stage is the wound bed preparation including primary wound expansion,removal of necrotic tissue and incision of sinus wall, removal of deep necrotic bone and fibrotic scarred skin on the outer wall of steel plate to normal tissue on the outer edge of the wound, removal of precipitated peptone and purulent fur in the hole, periphery and bone space of the steel plate, and removal of tendon tissue with basal necrosis and disintegration of the wound. After vacuum sealing drainage (VSD) 1~2 weeks, the peritraumatic basal granulation tissue grew well and there was no necrotic tissue in the wound. In the second stage, the exposed bone was covered with artificial dermis, the steel plate hole or the periphery and the basal space were filled, and the exposed steel plate was completely embedded, and then the fascia sleeve flap was transplanted to cover the wound. The sural neurovascular flap was performed in 6 cases and the lateral superior malleolar artery perforator flap in 1 case. Results:The flap survived well in all 11 cases. During the follow-up of 6 months to the removal of the plate, there was no case of rupture, exposure and sinus formation.Conclusions:Artificial dermal covering combined with fascial sleeve flap transplantation can effectively avoid wound dehiscence or sinus formation caused by foreign body retention, infection and flap contracture,It has good effect in repairing chronic wounds with bone plate exposure after severe trauma of lower limbs,.
Seasonal Drought Induces Hydraulic Dysfunction, Not Carbohydrate Depletion, for Robinia Pseudoacacia in a Semi-humid Forest
Authors: Zhang Qingyin , Jia Xiaoxu , Shao Mingan 
Published: October 2021

Abstract
BackgroundShifts in rainfall patterns that are associated with climate change are likely to cause widespread forest decline in regions where droughts are predicted to increase in duration and severity. However, causes of forest decline and their physiological mechanisms remain unclear, particularly the roles of carbon metabolism and xylem function. To explore the response of hydraulic architecture and non-structural carbohydrates (NSC) traits under seasonal drought, we conducted a manipulation experiment in a Robinia pseudoacacia plantation in 2015 and 2016 in Loess Plateau of China. Sap-flow, leaf area index, water potential, non-structural carbohydrate concentrations, and hydraulics in different organs were measured. ResultsThe mean pre-dawn and midday leaf water potential after two growing seasons of drought stress was significantly lower (-2.2 MPa and -2.7 MPa, respectively) than those of control trees (-1.5 MPa and -2.0 MPa, respectively). Drought stress accelerated the loss of conductivity, and promoted the formation of narrow hydraulic safety margins, which indicated that hydraulic failure could be a good predictor of “physiological drought” in trees when subjected to two growing seasons of drought. Both sugar and starch concentrations in stems and roots were similar in all trees throughout the drought period, which indicated that trees maintained good coordination between carbon supply and demand when confronted with two growing seasons of drought.ConclusionsOur results emphasized that hydraulic failure plays the predominant role in causing tree death during highly intense drought, while whether "carbon starvation" occurs during tree mortality remains to be tested in longer (multi-year) but less intense drought.
Bioconversion of Cattle Manure by Hermetia Illucens Larvae: Mineral Content Changes in Manure and Larval Biomass.
Authors: Sanchez-Matos Joan , de Aráujo Lara Pinto , Silva Vinnícius Henrique Cerqueira da , Lôbo Ivon Pinheiro , de Jesus Raildo Mota , Neto Jose Adolfo de Almeida 
Published: October 2021

Abstract
Bioconversion by Hermetia illucens larvae is a novel technology for organic waste treatment and valorization. However, since the possible uses of products from this process are in agriculture and livestock, the bioconversion must guarantee the mineral quality of both the Hermetia illucens larvae frass and larval biomass. Therefore, this study aimed to assess the mineral content changes for both the larval biomass and larvae frass of Hermetia illucens after the manure bioconversion to determine their suitability as animal feed and organic fertilizer, respectively. Hermetia illucens larvae were put into a plastic box containing fresh cattle manure, and the control treatment with the same conditions without larvae was established. After the first pre-pupae were detected, frass and larvae were collected, and their mineral content was analyzed. At the end of the experiment, the larvae showed increases in some micro and macronutrients, especially calcium and manganese, increasing up to 2.6 and 22.6 times the initial concentration, respectively. The toxic elements concentration was increased in larval biomass, but these levels met the international legislation for animal feed. As a result, the mineral content in larval biomass revealed that Hermetia illucens could be potentially used as animal feed, which could be comparable with fish meal, and is probably better than soybean meal. However, the larvae frass could only be used as organic fertilizer in a Canadian context, with further treatment for decreasing the chromium content being necessary.
Critical Care Retrieval in the Middle East: Descriptive Analysis of a Multidisciplinary Adult Critical Care Transfer and Retrieval Service in Qatar
Authors: Howard Ian , Steyn Rohan , George Steven , Thomson Wayne , Abdaljawad Wael , Hassan Ibrahim Fawzy , Castle Nicholas , Shaikh Loua Al , Owen Robert 
Published: October 2021

Abstract
Introduction The regionalisation of critical care resources has led to an increase in the need to transfer patients between facilities. The advent and implementation of critical care transfer and retrieval services have been the bridge to this divide, lying at the confluence of prehospital emergency care, in-hospital emergency medicine, and intensive care. Within the State of Qatar, the concept of critical care transfer and retrieval is a relatively new. Consequently, we conducted a retrospective cross-sectional study of all transfer and retrieval activity of a dedicated multidisciplinary transfer and retrieval service to better understand the use of these services in the region. Methods Extracted patient care record data were analysed and described using univariate and multivariate descriptive statistics. A log-binomial regression model with robust variance estimator was used to calculate crude and adjusted prevalence ratios for intubation status and arteriovenous access; and intubation status and medication combination, adjusting for age and gender for each model. Results Amongst the completed cases, the majority were male (60.39%), and within the 40-59 (27.7%) age group. Amongst the cases transferred, those with a primary respiratory pathology were the most common (19.59%), followed by cardiovascular patients (18.5%). Half of all patients had a self-maintained airway (51.6%), followed by a third who had an endotracheal tube in situ (36.74%). Midazolam was the most common hypnotic administered (51.27%), as was Fentanyl (88.02%) amongst the analgesic medications, and Noradrenaline amongst the inotropes (72.77%). Intubated patients had the highest proportion of severe and critical patients; patients transported with a Doctor; patients with multiple routes of arterial and/or venous access; and patients receiving any hypnotic, analgesic or inotrope, or a combination thereof. Conclusion The transfer and retrieval of critical care patients across Qatar is a relatively common occurrence. Variations in patient type and severity and the expectations of the transfer team, are significant. Variation in airway type and ventilation modalities, types and combinations of hypnotic, analgesic and inotropes used, and the multitude of arteriovenous access points observed in this study directly contributed towards the complexity of moving these patients from one facility to another.
Investigation of Mechanical and Biological Properties of FDM 3D-Printed PLA Scaffolds With Radial Gradient Porosity for Tissue Engineering
Authors: Eryildiz Meltem 
Published: October 2021

Abstract
Scaffolds with gradient porosity have become very promising candidates for tissue engineering and bone implants because of the combination of better mechanical and biological requirements. In this paper, a novel approach is proposed to design bone scaffolds with gradient porosity similar to the structure of cortical and spongy (cancellous) bones. The radial gradient PLA scaffolds were designed to consist of three different regions with the gyroid infill and, fabricated by Fused deposition modeling (FDM). The biological and mechanical properties of the scaffolds were investigated in vitro. Dense scaffold (G100) had improved mechanical properties but showed decreased bone ingrowth properties. In addition, porous scaffolds provided enhanced biological properties but decreased in mechanical strength (G40-G20). The scaffolds with radial gradient porosity (G100-40-20) gave highest cell proliferation. Because, mean pore size is an important aspect of scaffolds for mimicking bone.
The Prevalence of Thyroid Dysfunction in PCOS Women in Comparison with A Control Group
Authors: Ghazi Suad M. , ismail Nadia khaleil 
Published: October 2021

Abstract
Background

One of the common disorders that affect females of reproductive age is the Poly-Cystic Ovarian Syndrome (PCOS). Thyroid dysfunction is also more prevalent in this age group. This study aimed to evaluate the prevalence of thyroid dysfunction in women with PCOS in comparison with control and to investigate the association between hypothyroidism and PCOS in women of ovulatory age.
Patients and Methods:

Eighty female subjects of fertility age were selected and divided into two equal groups. The first 40 subjects are healthy females as a control group. The second 40 group are patients with PCOS. The PCOS women were diagnosed by Rotterdam criteria and performed thyroid tests, sex hormones and lipid profile tests.
Results

The results show that PCOS women with hypothyroidism are tending have a high body mass index (BMI). Forty-five percent of PCOS patients had hypothyroidism which was investigated in TSH and T4 tests by 35% and 10%, respectively. A high level of testosterone was found in females with PCOS. The cholesterol shows a significant correlation with the alteration in Thyroid Stimulating Hormone (TSH).
Conclusion

There is a correlation between thyroid dysfunction especially hypothyroidism with the polycystic ovarian syndrome because both of them lead in features to metabolic syndrome and there is a high prevalence of hypothyroidism in women with PCOS
Loss of HAS2 Confers Acquired Antiestrogen Resistance by Upregulating Ezrin Expression in ER-Positive Breast Cancer
Authors: Sun Xiaodan , Tang Fen , Liu Yiwen , He Yiqing , Du Yan , Zhang Guoliang , Gao Feng , Yang Cuixia 
Published: October 2021

Abstract
Background: Resistance to endocrine therapy is a major challenge for estrogen receptor-positive (ER+) breast cancer patients, but the underlying mechanisms remain unclear. Methods: Loss of hyaluronan synthase 2 (Has2) in adaptive resistant cells to tamoxifen and fulvestrant was observed by immunblotting assay. CRISPR/Cas9 technology was used to knock out Has2 in MCF7 cells to verify the effect of Has2 on the expression of ER and Ezrin and Akt and MAPK/ERK signaling routes. We utilized an Ezrin small-interfering RNA and Ezrin inhibitor to inhibit Ezrin expression for evaluating Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant treatment.Results: In this work, we showed that a Has2-loss state was acquired from adaptive resistance to tamoxifen and fulvestrant in luminal BrCas. Notably, the adapted loss of Has2 induced acquired resistance to antiestrogens in estrogen receptor (ER)-positive breast cancer cells through up-regulating the expression of Ezrin. Furthermore, we found that the loss of Has2 promoted while the consequent increase of Ezrin inhibited ERα expression/activity through the Akt and MAPK/ERK signaling routes, indicating an opposite effect on ERα expression during the development of antiestrogens-resistance. Inhibition of Ezrin reversed Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant, suggesting a Has2-Ezrin-ER negative-feedback loop in governing cellular sensitivity to tamoxifen or fulvestrant in luminal-like breast cancer cells. Finally, Knockdown or inhibition of Ezrin restored sensitivity to antiestrogens, implying that Ezrin could be a potential therapeutic target to tackle endocrine resistance. Conclusions: Taken together, our findings provide a direct relationship between ERα and Has2 implicated in resistance to endocrine therapy and a new insight into how ERα-signaling is regulated upon antiestrogens treatment, suggesting a novel therapeutic target for ER-positive breast cancer.
Effect of Climatic Gradient on Species Diversity and Structure of Faidherbia Albida Parkland in Burkina Faso (West Africa)
Authors: TRAORE Innocent Charles Emmanuel , Zaré Alhassane , Bondé Loyapin , Doré Djaka , Hien Bossila Séraphin , Ouédraogo Oumarou 
Published: October 2021

Abstract
Background: Faidherbia albida parkland provide many ecosystem goods and services to people, ranging from provisioning to regulating services. Despite this importance, these parks are degraded due to overexploitation, which is accentuated by the adverse effects of climate change. In this context of climate change, little information exists on the state of these F. albida parkland, particularly their diversity and structure along the North-South climatic gradient. This study aims to determine the effect of climatic gradient of F. albida parklands in order to provide updated data for sustainable management. Floristic diversity and population structure were assessed through forest inventories in the three climatic zones of Burkina Faso. Thus, 99 circular plots of 1 ha were sampled in three zones, including 33 per climatic zone. The data processing concerned the calculation of diversity indices (Shannon, Piélou equitability, and species richness), importance value indices, and the calculation of the means of the dendrometric parameters. The analyses were based on the comparison of medians using the Kruskal-wallis test, and the effect of the climatic gradient on the different variables was tested using generalized linear models. All of these analyses were carried out using the R.4.0.5 software. Results: The dendrometric parameters and floristic diversity vary significantly (Pv˂0.05) according of climatic zone. Furthermore, based on ecological importance value indices and diameter class structure, the majority of dominant species (11) show a regressive dynamic in all three climatic zones characterized by abundance of old individuals at expense of young ones. This reflects an ageing and continuous destruction of these parklands. However, species such as V. tortilis (Sahelian zone) and A. indica (Sudano-Sahelian and Sudanian zones) show good dynamics. As for F. albida, its structure is regressive in all climatic zones.Conclusion: Although the climatic gradient has a significant effect on the diversity and species structure of F. albida parkland, their current dynamics are thought to be due to unsustainable management of local populations.
Potential molecular mechanism of Yishen capsule in the treatment of diabetic nephropathy based on network pharmacology and molecular docking technology
Authors: Hu Yaling , Liu Shuang , Liu Wenyuan , Zhang Ziyuan , Liu Yuxiang , Li Sufen , Sun Dalin , Zhang Guang , Fang Jingai 
Published: October 2021

Abstract
Background : Using network pharmacology and molecular docking technology to explore the mechanism of Yishen capsules in the treatment of diabetic nephropathy. Methods: Active components of Yishen Capsules were obtained using database such as TCMSP and TCMID, and diabetic nephropathy targets were obtained from databases such as Gencards, OMIM, DisGeNET. A network of "Yishen Capsule Components-Diabetic Nephropathy Targets-Pathways" was constructed by analyzing data above to screening out core targets for molecular docking verification. Finally, a rat model of diabetic nephropathy was generated, and renal tubular epithelial cells were extracted and cultured under high glucose conditions. Based on these experimental models, the key signal pathway target protein genes screened by network pharmacology were verified both in vitro and in vivo. Results: The main active components of Yishen Capsule in the treatment of diabetic nephropathy include quercetin, kaempferol, gallic acid, astragaloside IV and so on. Some key targets (such as AR, AKT1, TP53, ESR1, JUN) and important signal pathways (such as AGE-RAGE signal pathway, HIF-1 signal pathway and JAK-STAT signal pathway) were included in the treatment of diabetic nephropathy of Yishen Capsule. Molecular docking assay showed that most of the targets have good binding activity with the components of Yishen Capsules. Based on the results of network pharmacology, key target proteins in HIF-1α and JAK2/STAT3 signaling pathways were selected for experimental verification. Results presented that HIF-1α, JAK2, STAT3, TGF-β and MCP were increased under high glucose environment. With the treatment of Yishen Capsule, the expression of HIF-1α further increased, while the expression of JAK2, STAT3, MCP-1 and TGF-β were decreased. Conclusions : This study revealed the mechanism of Yishen Capsules in the treatment of diabetic nephropathy, which possesses the characteristics of multi-component, multi-target, and multi-pathway. Further experiments confirmed that Yishen Capsules interfered with HIF-1α and JAK/STAT signaling pathways to reduce inflammation and fibrosis damage in the kidney tissue of rats with diabetic nephropathy. Key Words: Diabetic Nephropathy(DN); Network pharmacology; Molecular docking;HIF-1α; JAK/STAT
Mindfulness Training led to Catharsis and Mindfulness-To-Meaning in a Patient with Social Anxiety Disorder Comorbid with Persistent Depressive Disorder (Dysthymia) - A Case Report with Patient's Personal Note -
Authors: Kaiya Hisanobu , Hasegawa Asuka , Komatsu Chika , Noda Shota , Minami Fusaka , Goldin Philippe , Hasegawa Yosuke 
Published: October 2021

Abstract
Background:The use of mindfulness in the treatment of anxiety and depression is becoming more and more widespread, but there are few reports that it has fundamentally changed patients' sense of life. Case Presentation:This report showed 9 year’s progress of treatment for Social Anxiety Disorder and Persistent Depressive Disorder in a 41year-old female patient experiencing a long-term painful married life with a carefree husband and controlling mother-in-law. During the past 25 months, she received 180 sessions of mindfulness training, resulting in complete remission of the anxiety and depression symptoms. After the 30th session she experienced an episode of the catharsis during the mindfulness training and experienced what can be called Mindfulness-To-Meaning, which is characterized by durable eudaimonic well-being in the face of adversity. Discussion and Conclutions:The psychological mechanisms that supported arriving at Mindfulness-To-Meaning were delineated as: Heightened (1) awareness which promoted (2) verbalization of the primary theme of suppression, in which the stress was (3) appraised metacognitively. Then (4) decentering permitted integrating this painful theme into a new adaptive self and world view, which resulted in (5) emotional catharsis, or release. Furthermore (6)　positive appraisal of this experience enhanced awareness and led to the experience of (7) Mindfulness-To-Meaning .