bluuebunny/crossref_metadata_2025_split huggingface dataset:
DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae
Authors: Zhang Xiaojuan , Liu Yao , Liu Weiwei , Shen Yuhu , Cao Fangqi , Deng Yunxia , Cao Zhangjun , Huang Zhiwei 
Published: February 2025

ABSTRACTGemfibrozil (GEM) is a phenoxy aromatic acid‐based lipid‐lowering drug. It activates peroxisome proliferator‐activated receptor alpha (PPAR‐α), which leads to altered lipid metabolism and lowers serum triglyceride levels by modulating lipoprotein lipase. However, the action of the mode of GEM is still unclear. Herein, the model organism Saccharomyces cerevisiae was applied to explore the molecular mechanism of GEM regulating lipid metabolism. The results showed that the triacylglycerol (TAG) content and the number of lipid droplets of yeast increased significantly after GEM treatment in the wild‐type BY4741. Screening of mutations related to lipid metabolism pathways (PAH1, DGK1, TGL3, TGL4, LRO1, ARE1, ARE2, and DGA1) showed that dgk1Δ had no change in lipid accumulation under GEM. In the wild type, GEM inhibited the expression of DGK1, resulting in a significant decrease in the contents of phospholipids (phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS)) and neutral lipids (TAG and diacylglycerol (DAG)). However, their abundances could not be changed in dgk1Δ after the treatment with GEM Luciferase assay further showed that DGK1 may be a target of GEM to induce lipid accumulation via TUP1/CYC8, which could act on the DGK1 promoter‐TATA highly conserved element (‐400 bp – 200 bp). Altogether, the effect of GEM on lipid metabolism was associated with the upregulation of TUP1/CYC8, leading to a decrease in the expression of DGK1, thereby increasing the TAG content in yeast cells. It is expected that the data will help to clarify the molecular mechanism of GEM regulating lipid metabolism in humans.
Nutritional management and outcomes of malnourished children under 2 years with intestinal failure
Authors: Pavanelli Estela Cristina , Neto Joao Seda , Farah Beatriz Camilo , Longo Roberta Luiza , Person Natália Canale , Gritti Catiana Mitica , de Jesus Carine Dias Ferreira , de Carvalho Camila Mascarenhas Teixeira , Hayashi Massami , Gameiro Catherina Oliveira , Brufato Jackeline Farias , Blini Gabrielle Marques , Vincenzi Rodrigo 
Published: February 2025

AbstractObjectiveThis study aimed to assess the evolution of nutritional status, parenteral nutrition (PN) composition, and PN dependence index (PNDI) in children under 2 years old with intestinal failure (IF) and malnutrition.MethodsThis retrospective cohort included patients admitted between November 1, 2016, and August 31, 2021, with IF, age ≤24 months, and weight‐for‐age (Wt/A) <−2 standard deviation with a follow‐up of 12 months. Data collected included demographic, anthropometric, biochemical, and nutritional information. The PNDI was compared between enteral autonomy (EA) and nonenteral autonomy (non‐EA) groups.ResultsThirty patients (16 males, 53.33%) were included, and short bowel syndrome (SBS) was the leading cause of IF (n = 27, 90%). The mean age at admission was 7.23 ± 3.90 months, and 36.66% of the patients had a conjugated bilirubin (CB) level >2 mg/dL; 87% had a Wt/A z‐score <−3 and 90% had a height‐for‐age (Ht/A) z‐score <−3. At 5 months, the mean Wt/A z‐score improved to −1.82. At admission, median protein, lipid, and glucose intake on PN were 3, 1.6, and 17.27 g/kg/day, respectively. In the PNDI analysis, the EA group had a mean index of 141% versus 158% for the non‐EA group at admission, with no statistical differences. From the sixth month onward, the EA group showed a significantly lower PNDI (50% vs. 108% for non‐EA patients; p = 0.032). Both groups demonstrated adequate nutritional recovery.ConclusionAdequate multidisciplinary care can reverse severe malnutrition, and the PNDI may be a predictor of EA.
Safety and Efficacy of a Protocol for In‐Office Pediatric Aural Foreign Body Removal
Authors: Prasad Dilan , Isaacson Glenn 
Published: February 2025

ABSTRACTObjectivesTo examine the safety and efficacy of a step‐by‐step protocol for the office removal of aural foreign bodies (FB) in a pediatric population.MethodsAll children presenting to an academic pediatric outpatient department were identified from a computerized collection of office notes and operative reports. FB removal was performed in a standard fashion including: (1) pre‐procedure discussion with shared decision making, (2) restraint in a supine position with a trained second person holding the head, (3) FB removal using an operative microscope and instrumentation. A case was considered a failure if subsequent removal under sedation or general anesthesia was required.Results447 total cases (ears) were identified in 428 children (19 had bilateral FBs). 201/428 (47%) of children had previous attempts at FB removal elsewhere. 431/447 (96.4%) of FBs were successfully extracted in the office. 16/447 (3.6%) of FBs were removed in the operating room. Out of the 431 successful removals, two patients had minor complications (ear canal lacerations). No child suffered a more severe complication. The failed removal group was more likely to have a documented neurodevelopmental disorder (4/21 vs. 12/407, p = 0.0001).ConclusionThis protocol resulted in a high rate of successful FB removal, even in children with prior failed attempts. There were no significant ear injuries in this 22‐year experience, which included children with attention‐deficit/hyperactivity disorder (ADHD) and autism spectrum disorder. This approach should be considered by otolaryngologists who care for children in the office setting.Level of Evidence3—Retrospective review.
The Nexus Between Intensive Land Utilization, Energy Efficiency, and Economic Growth: Application of Advanced Econometric Approaches
Authors: Waheed Rida , Aziz Ghazala , Khan Mohd Saeed 
Published: February 2025

ABSTRACTThe aim of this study is to analyze Saudi Arabia's economic transformation, emphasizing the period from 1990 to 2022 and considering the implementation of Vision 2030. Land use, energy consumption, and economic growth are evaluated through the use of a nonlinear ARDL model. Based on the findings, the Saudi economy has shifted from relieving heavily on oil revenues to diversification and sustainability. The economy has been positively impacted by sustainable land use practices and a reduction in nonrenewable energy consumption since Vision 2030, which highlights the early successes of diversification efforts. The study demonstrates a strong link between energy efficiency enhancements and economic performance, highlighting the economic benefits of ongoing energy reforms. However, the Saudi economy's sensitivity to oil prices persists, albeit with a diminished impact post‐Vision 2030. The research emphasizes the importance of strategic land management and investment in energy‐efficient technologies to foster a resilient and diversified economic structure.
Targeting CDK4/6 suppresses colorectal cancer by destabilizing YAP1
Authors: Wen Yalei , Yang Xiao , Li Shengrong , Huang Lei , Chen Jiayi , Tan Lirong , Ma Xiuqing , Zhu Yingjie , Li Zhengqiu , Shan Changliang , Zhang Chunze , Zhang Qiushi , Liang Mingchao , Zhang Haoxing , Liu Tongzheng 
Published: February 2025

AbstractColorectal cancer (CRC) is among the most prevalent and deadly cancers worldwide. The Yes‐associated protein 1 (YAP1) is frequently dysregulated in cancers, contributing to cancer stemness, chemoresistance, and cancer‐related death. However, strategies directly targeting YAP1 have not yet been successful because of the lack of active binding pockets and unregulated toxicity. In this study, our Food and Drug Administration (FDA)‐approved drug screening reveals that abemaciclib, a cyclin‐dependent kinase 4/6 (CDK4/6) inhibitor, dramatically promotes the proteasome‐dependent degradation of YAP1, thereby inhibiting tumor progression in CRC cells and patient‐derived xenograft models. We further identify deubiquitinating enzyme 3 (DUB3) as the bona fide deubiquitinase of YAP1 in CRC. Mechanistically, CDK4/6 directly phosphorylates DUB3 at Ser41, activating DUB3 to deubiquitinate and stabilize YAP1. Conversely, loss of Ser41 phosphorylation by CDK4/6 inhibition or Ser41A mutation, promotes YAP1 degradation and suppresses YAP1‐driven tumor progression. Histological analysis shows a positive correlation between DUB3 and YAP1 expression in CRC specimens. Collectively, our study uncovers a novel oncogenic role of the CDK4/6‐DUB3 pathway, which promotes YAP1 stabilization and tumor‐promoting function, highlighting that targeting CDK4/6 offers a potential therapeutic strategy for CRC with aberrantly upregulated DUB3 and YAP1.
Cooked Broccoli Alters Cecal Microbiota and Impacts Microbial Metabolism of Glucoraphanin in Lean and Obese Mice
Authors: Zhao Anqi , Li Jiaxuan , Peterson Mark , Black Molly , Gaulke Christopher A. , Jeffery Elizabeth H. , Miller Michael J. 
Published: February 2025

ABSTRACTScopeBrassica vegetables contain unique compounds known as glucosinolates (GSLs), which, when hydrolyzed by plant or microbial myrosinase, form bioactive isothiocyanates (ITCs) that offer health benefits to the host. The present study evaluated the impact of cooked broccoli (broccoli myrosinase inactivated) consumption on cecal microbial metabolism of glucoraphanin (GRP) in lean and obese mice and characterized the changes in cecal microbiota following broccoli‐containing diets.Methods and resultsTwenty lean and 20 diet‐induced obese (DIO) mice were randomized to consume control or cooked broccoli supplemented diets for 7 days. Cooked broccoli consumption increased ex vivo microbial GRP hydrolysis by cecal contents collected from lean and obese mice, led to increased production of sulforaphane (SF), sulforaphane‐cysteine (SF‐CYS), total ITC, and colonic NAD(P)H: Quinone Oxidoreductase (NQO1) activity. Further investigation revealed increased abundance of health‐promoting gut microbiota, including Lachnospiraceae NK4A136 group and Dubosiella newyorkensis, following broccoli‐containing diets. The Peptococcaseae family, the Blautia genus, and an amplicon sequence variation (ASV) from the Oscillospiraceae family exhibited negative correlation with total ITC production.ConclusionThese finding suggest that cooked broccoli consumption enhances microbial GRP hydrolysis to produce more bioactive ITCs and inform future strategies toward altering microbial GSL metabolism to promote gut health in both lean and obese individuals.
A Compact 2.05 μm MOPA Laser With SLM Operation
Authors: Liu Wei , Ju Youlun , Dai Tongyu , Liu Qiang , Liu Chao 
Published: February 2025

ABSTRACTWe present a single longitudinal mode Ho:YLF MOPA laser operating at 2050.7 nm by utilizing the Fabry–Perot etalon method to construct a compact SLM seed laser and employing Tm‐doped fiber as the amplifier. With an output power of 336 mW generated by the seed laser, the maximum output power of 13.1 W for the single longitudinal mode MOPA laser was recorded at a pump power of 88.7 W. A peak slope efficiency of 26.9% was achieved when the pump power exceeded 58.7 W. To our knowledge, this is the first report of utilizing Tm‐doped fiber to amplify the SLM operation of Ho:YLF laser.
Adherence to self‐monitoring and behavioral goals is associated with improved weight loss in an mHealth randomized‐controlled trial
Authors: Burke Lora E. , Bizhanova Zhadyra , Conroy Molly B. , Cheng Jessica , Beatrice Britney , Kariuki Jacob K. , Parmanto Bambang , Sereika Susan M. 
Published: February 2025

AbstractObjectiveThe SMARTER mobile health (mHealth) weight‐loss trial compared adherence to self‐monitoring (SM) of diet, physical activity (PA), and weight and adherence to study‐prescribed diet and PA goals between SM + feedback (SM + FB) and SM‐only arms over 12 months.MethodsParticipants used digital tools to monitor their dietary intake, PA, and weight. We applied generalized linear mixed modeling to compare patterns of monthly adherence to SM and behavioral goals between groups over time and examine the association of adherence to SM and behavioral goals with ≥5% weight loss.ResultsThe sample (N = 502) was 80% female and 82% White, with a mean (SD) BMI of 33.7 (4.0) kg/m2. Adherence to SM and fat, calorie, and PA goals declined nonlinearly over time, with the SM + FB group displaying less of a decline compared with the SM‐only group. Higher adherence to diet, PA, and weight SM and to calorie and PA goals was associated with greater odds of achieving ≥5% weight loss. A higher monthly probability of achieving ≥5% weight loss was associated with greater adherence to diet, PA, and weight SM and to calorie and PA goals.ConclusionsThese results suggest that future research should examine the mechanisms underlying tailored FB to improve the effect of FB intervention strategies that can lead to improved weight loss.
Validation of Clinical Practice Research Datalink (CPRD) Aurum Mother‐Baby Link
Authors: Vasilakis‐Scaramozza Catherine , Persson Rebecca , Kafatos George , Neasham David , Hagberg Katrina Wilcox , Jick Susan 
Published: February 2025

ABSTRACTPurposeAssess the validity of the Clinical Practice Research Datalink (CPRD) Aurum Mother‐Baby Link (MBL).MethodsWe assessed the validity of CPRD Aurum MBL pairs using several strategies. Our study population was based on all available MBL pairs in a 100‐practice sample of CPRD Aurum. We sent a questionnaire to the general practitioner of a sample of MBL patients asking if linkage was correct and whether delivery date was correct within 14 days. We also compared MBL delivery date to the matched child's birth date in CPRD Aurum. Finally, in the Hospital Episode Statistics (HES) eligible population, we compared MBL delivery date to birthing parent's HES delivery date.ResultsThe study population included 124 962 MBL pairs: 124 962 livebirths, 91 218 birthing parents, and 123 562 deliveries. Based on 361 questionnaire responses standardized to the MBL population, 85% (95% CI 74%–96%) of pairs were confirmed as correctly matched, <1% (0.04%–1.4%) were incorrectly matched, and 13% (10%–18%) could not be confirmed. For 71% (61%–82%) of the sample, delivery date was correct within 14 days. Among the entire population of 124 962 MBL pairs, 73% of MBL pairs had MBL delivery date within the baby's birth month recorded in CPRD Aurum. Among 79 834 patients with HES linkage, 94% of 107 080 MBL deliveries in CPRD Aurum were confirmed in HES.ConclusionsOur various assessments of CPRD Aurum MBL found the great majority of pairs were correctly matched and most delivery dates were correct within 14 days. Further refinement of delivery dates may be necessary for some studies.
Impacts of assimilating polarimetric radar KDP observations with an ensemble Kalman filter on analyses and predictions of a rainstorm associated with Typhoon Hato (2017)
Authors: Shu Aiqing , Shen Feifei , Li Hong , Min Jinzhong , Luo Jingyao , Xu Dongmei , Chen Jiajun 
Published: February 2025

AbstractThe direct assimilation of polarimetric‐radar specific differential phase KDP observations is realized with an ensemble Kalman filter within the Gridpoint Statistical Interpolation system. The landfall of Typhoon Hato is chosen as a case study to investigate the added value of assimilating polarimetric variable KDP in improving analyses and predictions of a short‐term rainstorm associated with the typhoon through three experiments. A control experiment without data assimilation is carried out, along with two radar data assimilation experiments. One of them assimilates reflectivity ZH and radial velocity RV from the Yangjiang radar at 6 min intervals within a 1 hr assimilation time frame, and the other assimilates specific differential phase KDP on top of ZH and RV. The results show that the wind convergence is generated at the lower layer and the rainwater mixing ratio qr content is enriched in the location where the heavy rainstorm occurred when KDP observations are additionally assimilated. It seems that favorable dynamical and moisture conditions are provided with the assimilation of KDP to adjust the location and magnitude of the rainfall center. Furthermore, both the analyzed and predicted composite reflectivity match best with the observations due to the more precise depiction of the hydrometeor qr with the introduction of polarimetric variable KDP. In the final deterministic forecast initialized by the ensemble mean analysis, not only the location but also the intensity of the rainfall center best fits the observation with the assimilation of KDP. To be specific, the experiment assimilating KDP yields comparatively the highest forecast skill with threat‐score values consistently greater than 0.13. However, its bias scores show that the rainfall at 25 mm threshold is overestimated, whereas the rainfall at 125 mm threshold is underestimated. The improved forecast skill can also be proved by the quantitative threat score and fraction skill score using percentile‐based thresholds.
Nuclear Factor of Activated T Cells Signalling and Viral Pathogens: A Dynamic Cross‐Talk
Authors: Khanizadeh Sayyad , Shahzamani Kiana , Nakhaie Mohsen , Pormohammad Ali , Talei Gholamreza , Mirzaei Habibollah 
Published: February 2025

ABSTRACTThe signalling pathway of the nuclear factor of activated T cells (NFAT) plays a crucial role in regulating various cellular processes such as cardiac hypertrophy, adipose differentiation, chondrocyte development, angiogenesis, inflammation, immune system activation, organogenesis, cancer cell migration, differentiation and survival. In addition, the NFAT signalling pathway acts as a key regulator of viral infections. Accordingly, it is plausible to assume that viruses have developed different mechanisms to manipulate this pathway to promote their pathogenicity. Viral pathogens can either inhibit or upregulate NFAT signalling through various mechanisms, including modulation of calcineurin activity, calcineurin/NFAT interaction, NFAT stability and translocation, NFAT‐DNA–binding activity and NFAT–transcription partner interaction. Therefore, the NFAT signalling pathway can be regarded as a promising target to control viral infections. This review discusses the dynamic interactions between the NFAT signalling pathway and viral pathogens. It also addresses several drugs and agents that can target the NFAT signalling pathway at different levels to control viral infections.
Multilanguage Detection of Design Pattern Instances
Authors: Andrade Hugo , Bispo João , Correia Filipe F. 
Published: February 2025

ABSTRACTCode comprehension is often supported by source code analysis tools that provide more abstract views over software systems, such as those detecting design patterns. These tools encompass analysis of source code and ensuing extraction of relevant information. However, the analysis of the source code is often specific to the target programming language. We propose DP‐LARA, a multilanguage pattern detection tool that uses the multilanguage capability of the LARA framework to support finding pattern instances in a code base. LARA provides a virtual AST, which is common to multiple OOP programming languages, and DP‐LARA then performs code analysis of detecting pattern instances on this abstract representation. We evaluate the detection performance and consistency of DP‐LARA with a few software projects. Results show that a multilanguage approach does not compromise detection performance, and DP‐LARA is consistent across the languages we tested it for (i.e., Java and C/C++). Moreover, by providing a virtual AST as the abstract representation, we believe to have decreased the effort of extending the tool to new programming languages and maintaining existing ones.
Dynamic Response Prediction of a Cantilever Beam Under Different Boundary Constraints and Excitation Conditions Based on an Improved Physics‐Informed Neural Network
Authors: Zhang Xinyu , Zhu Hao , Xu Wei 
Published: February 2025

ABSTRACTThe cantilever beam structures, like wind turbine towers, space masts, solar wings, and high‐rise chimneys and buildings, are widely used engineering structures. It is crucial to fast and accurately predict their dynamic responses under complicated excitations. This paper establishes an improved physics‐informed neural network (PINN) called Fourier transformation‐PINN (FT‐PINN) for predicting the dynamic response of a cantilever beam subject to different boundary constraints and excitation conditions. The core idea of the FT‐PINN is to use the Latin hypercube sampling strategy for generating model training points and introduce multiple sets of control equations with different frequencies through Fourier expansion to achieve high solving accuracy and efficiency for partial differential equations. Two loss functions, including the mean square error and mean absolute error, are included in the FT‐PINN for comparison. Four test cases are designed to evaluate the performance of the FT‐PINN and classic PINN in solving dynamic equations of a cantilever beam structure with different boundary and excitation conditions. It is validated that the FT‐PINN model proposed in this paper has higher accuracy and efficiency than the classic PINN. This also provides a new approach for using PINN to handle local sharp gradients and complex high‐frequency problems in vibration equations.
Boundedness of the Fifth Derivative for the One-Particle Coulombic Density Matrix at the Diagonal
Authors: Hearnshaw Peter 
Published: February 2025

Abstract
Boundedness is demonstrated for the fifth derivative of the one-particle reduced density matrix for non-relativistic Coulombic wavefunctions in the vicinity of the diagonal. To prove this result, improved pointwise bounds are obtained for cluster derivatives of wavefunctions involving multiple clusters.
5-Dimensional Malcev-Like Algebras
Authors: Ficzere Kornélia , Figula Ágota 
Published: February 2025

Abstract
The five-dimensional anti-commutative algebras having an analogous family of flags of subalgebras as the solvable Malcev algebras form the class of Malcev-like algebras. Recently a classification of the binary Lie algebras in this class is achieved. Our investigation extends this result. We determine Malcev-like algebras over a field

$${\mathbb {K}}$$

K

of characteristic zero. These algebras are extensions of

$${\mathbb {K}}$$

K

by a 4-dimensional nilpotent Lie algebra and simultaneously semidirect sums of the two-dimensional non-abelian Lie algebra and an abelian algebra. We find normal forms of their multiplications and describe their isomorphism classes.