bluuebunny/crossref_metadata_2025_split huggingface dataset:
La melancolía de Calisto
Authors: Morros Mestres Bienvenido 
Published: February 2021

Rojas evita mencionar la palabra melancolía porque no quiere incurrir en la contradicción de relacionar ese temperamento con la enfermedad de amor que ha decidido atribuir a su protagonista. Estaba al corriente de las ideas que al respecto habían formulado los médicos más importantes de la Edad Media. De todas ellas comulga con las que había sostenido Bernardo Gordonio en su obra titulada Lilio de la medicina. El profesor de Montpellier había creído que la terapia sexual no era demasiado eficaz para combatir la enfermedad de amor porque la provocaba un temperamento, el melancólico, poco apto para su práctica. En la Celestina, no acaba de estar claro por qué Calisto no se ha curado de su enfermedad: si por haber practicado poco o mucho la terapia sexual.
Rapport om tidlig marin ressursutnyttelse i Altafjord-området basert på tidlige historiske kilder
Authors: Henriksen Jørn Erik 
Published: February 2021

Rapporten er en kommentert gjennomgang av et bredt utvalg eldre skriftlige kilder, relevante faghistoriske studier og lokalhistorisk litteratur vedrørende utnyttelse av marine/akvatiske ressurser i Altafjordområdet. Siktemålet var å utrede opplysninger om hvilke ressurser som tradisjonelt har blitt utnyttet til hvilke tider og i hvilke deler av undersøkelsesområdet. Redskapstyper og fangstteknologi benyttet i utnyttelse av akvatiske ressurser, er så langt mulig redegjort for. Det antydes hvilke ressurser og fangstmetoder som kan ha vært anvendt også i forhistorisk tid.
Kildene ga inntrykk av en fiskerik fjord med de rikeste havfiskeriene i fjorden nord for Talvik, sommer som vinter. Håndsnørefiske var den dominerende fisketeknologien frem til 17-1800-tallet, men kildene viser variasjon i eldre fiskemetoder. Fiskerier i de grunnere fjordbunnene har i perioder vært økonomisk lønnsomme. Semi-passive fiskeredskaper har i forhistorien mest sannsynlig vært brukt på grunne havpartier.
Laksefiskeriene i Altaelva dominerer kildene fullstendig med hensyn til utnyttelse av anadrom fisk. Intensivt stengsel- og garn/notfiske dominerte, men bruk av tradisjonelle fiskemetoder som lystring og enklere varianter av stengselfiske fortsatte inn i moderne tid.
Kildene gir inntrykk av gode forekomster av fjordkobbe over det meste av fjorden. Områdene ved Rafsbotn og partier av Altaelva er eneste område av fjorden beskrevet som særskilt gode fangststeder for fjordkobbe.
Kildene fremhever de rike forekomstene av hval generelt i Altafjorden, men fokuserte spesielt på store bardehvalarter i området ved Bossekop – Rafsbotn og Talvik vinterstid.
Expression, purification, crystallization and preliminary X-ray crystallographic studies of a mitochondrial membrane-associated protein Cbs2 from Saccharomyces cerevisiae
Authors: Wu Dan , Zhu Guanyu , Zhang Yufei , Wu Yan , Zhang Chunlei , Shi Jiayi , Zhu Xiaofeng , Yuan Xiaohuan 
Published: February 2021

Background
Mitochondria are unique organelles that are found in most eukaryotic cells. The main role of the mitochondria is to produce ATP. The nuclear genome encoded proteins Cbs1 and Cbs2 are located at the mitochondrial inner membrane and are reported to be essential for the translation of mitochondrial cytochrome b mRNA. Genetic studies show that Cbs2 protein recognizes the 5′ untranslated leader sequence of mitochondrial cytochrome b mRNA. However, due to a lack of biochemical and structural information, this biological process remains unclear. To investigate the structural characteristics of how Saccharomyces cerevisiae (S. cerevisiae) Cbs2 tethers cytochrome b mRNA to the mitochondrial inner membrane, a preliminary X-ray crystallographic study was carried out and is reported here.

Methods
The target gene from S. cerevisiae was amplified by polymerase chain reaction. The PCR fragment was digested by the NdeI and XhoI restriction endonucleases and then inserted into expression vector p28. After sequencing, the plasmid was transformed into Escherichia coli C43 competent cells. The selenomethionine derivative Cbs2 protein was overexpressed using M9 medium based on a methionine-biosynthesis inhibition method. The protein was first purified to Ni2+-nitrilotriacetate affinity chromatography and then further purified by Ion exchange chromatography and Gel-filtration chromatography. The purified Se-Cbs2 protein was concentrated to 10 mg/mL. The crystallization trials were performed using the sitting-drop vapor diffusion method at 16 °C. The complete diffraction data was processed and scaled with the HKL2000 package and programs in the CCP4 package, respectively.

Results
Cbs2 from S. cerevisiae was cloned, prokaryotic expressed and purified. The analysis of the size exclusion chromatography showed that the Cbs2 protein peaked at a molecular weight of approximately 90 KDa. The crystal belonged to the space group C2, with unit-cell parameters of a = 255.11, b = 58.10, c = 76.37, and β = 95.35°. X-ray diffraction data was collected at a resolution of 2.7 Å. The Matthews coefficient and the solvent content were estimated to be 3.22 Å 3 Da-1 and 61.82%, respectively.

Conclusions
In the present study Cbs2 from S. cerevisiae was cloned, expressed, purified, and crystallized for structural studies. The molecular weight determination results indicated that the biological assembly of Cbs2 may be a dimer.The preliminary X-ray crystallographic studies indicated the presence of two Cbs2 molecules in the asymmetric unit. This study will provide an experimental basis for exploring how Cbs2 protein mediates cytochrome b synthesis.
Mental health status and quality of life in elderly patients with coronary heart disease
Authors: Tang Min , Wang Song-Hao , Li Hui-Lin , Chen Han , Sun Xin-Yi , Bian Wei-Wei , Sheng Jing , Ma Shao-Jun 
Published: February 2021

Background
Coronary heart disease (CHD) is the leading cause of morbidity and mortality among elderly individuals. Patients with CHD are at high risk for mental health disorders, and psychological issues may affect the quality of life (QoL) of these patients. Nevertheless, there is little evidence regarding the psychological issues and QoL of patients with CHD among the elderly population. This study aimed to investigate the relationship between comorbidities and mental status as well as QoL among elderly patients with CHD.

Methods
Overall, 216 patients were included in this cross-sectional, observational, single-center study. The demographics and clinical manifestations of the patients were collected from electronic medical records. All patients were interviewed using the Chinese version of Symptom Checklist 90 (SCL-90) to assess the mental health status and the World Health Organization Quality of Life-BREF questionnaire (WHOQOL-BREF) to assess the QoL.

Results
In total, 96 men and 120 women, with a mean age of 71.69 ± 8.30 years, were included. When controlling for the patients’ sex, marital status and stroke, multiple stepwise linear regression analyses suggested that for CHD patients, comorbid type 2 diabetes mellitus had the significant influence on average positive factors (Coef., 5.809; 95% CI [2.467–9.152] p = 0.001); when controlling for the patients’ sex, marital status and type 2 diabetes mellitus, multiple stepwise linear regression analyses suggested that for CHD patients, comorbid stroke had the significant influence on average positive factors (Coef., 8.680; 95% CI [4.826–12.534]; p < 0.001); when controlling for the patients’ sex, marital status, type 2 diabetes mellitus and stroke, multiple stepwise linear regression analyses suggested that for CHD patients, comorbid primary hypertension had the significant influence on phobic anxiety (Coef., 0.178; 95% CI [0.010–0.347]; p = 0.038).

Conclusions
For elderly CHD patients, comorbid type 2 diabetes mellitus and stroke were at risk for psychological problems and lower QoL. Our findings may guide patients and clinicians to make better decisions and achieve better outcomes.
Economic Indices for the Application of Irrigation to Enhance Cactus Pear Fruit Yield
Authors: Sánchez-Toledano Blanca Isabel , Zegbe Jorge A. 
Published: February 2021

Aims: Cactus pear cultivation for fruit production is under rainfed conditions, mainly. Hence, irrigation uses for this purpose is expensive in arid and semi-arid agricultural lands. The objective of this study was to derive agro-economic indicators associated with supplemental irrigation to improve fruit yield of commercial cactus pear cultivars grown in a semiarid agroecosystem of Mexico.
Study Design: The irrigation treatments tested were fully irrigated (FI) and supplemental irrigation (SI), with non-irrigated as a control (NI).
Place and Duration of Study: The experiment was set up at the Campo Experimental Zacatecas, Calera, Zacatecas, Mexico from 2011 to 2013.
Methodology: Five-year-old cactus pear plants of ‘Cristalina’ (O. albicarpa Scheinvar; white-pulped fruit) and ‘Roja Lisa’ [O. ficus-indica (L.) Mill.; red-pulped fruit] were included. The experiment was conducted in a split-block design. There were three blocks; each included both cultivars, randomly allocated to each irrigation treatment. The response variables were: fruit yield (FY), cultivation costs (CC), gross return (GR), net return (NR), benefit-cost ratio (BC), irrigation use efficiency (IUE), gross water productivity (GWP), net water productivity (NWP), labor productivity (LP), break-even-point (BEP), and credit vulnerability (CV).
Results: In all years but 2013, FI ‘Cristalina’ plants had the highest CC, reflected in their greater GR and NR; however, BC values were similar between SI and FI plants or greater in SI plants for 2013. Therefore, SI plants had the highest IUE, GWP, NWP, LP, EP, and equal or greater credit vulnerability than FI plants. The NI plants produced positive values for BEP and CV. The economic indices for ‘Roja Lisa’ were similar to those of ‘Cristalina’.
Conclusion: The SI treatment produced the best agricultural economic indices and it is also a feasible water-saving irrigation strategy for cactus pear cultivation in semiarid agroecosystems worldwide.
Analyzing the Impact of Long-Lasting Changes in Energy Homeostasis and Nutrient Sensing on Nutritional Programming of Hypothalamus in Rats
Authors: Aboelnaga Shimaa Mohammed Hasnin , Khatoon Fahmida 
Published: February 2021

Background: The diseases due to nutritional deficiencies or imbalanced diet can be due to different factors. These factors can be biological or environmental. The study indicated that there will be increase risks of acquiring diseases as a result of irregular intake of diet. These risks include the increased risk of diseases such as liver abnormality, hypertension, lipids deposition, and other diseases.
Methodology: The databases used for the collection of secondary information include Science Direct, PubMed, and Google Scholar. Besides, the keywords used for the searching of relevant research articles include “experimental rats, nutritional deficiencies. Hypothalamus, mechanisms studies, gene ontology, metabolic syndromes”. There were the use of female rats (n=8) and the weight was ranged between 200 to 250 grams. The experimental rats were placed in a cycle of 12 h light/dark. An adequate amount of water and food was provided for about one week to the rats before starting the experiment. Enzymatic procedures were used for the analysis of cholesterol, fatty acids, and triglycerides. Glucose concentrations in the blood samples were also assessed using the glucose meter. The insulin levels were also measured by using the assay kits. The hybridisation, scanning, and normalization of the data were also done. The extracted RNA from the hypothalamus was transcribed by using the “Superscript II RNAseH- Reverse-Transcriptase”. The t-test was also performed using statistical software. The groups of genes and their altered expressions were studied after the malnutrition conditions. The results obtained through PCR were expressed in the form of average and standard deviation values [1]. The mRNA expression levels in the samples of the hypothalamus were also assessed. The data were compared using the student test. The significance levels were measured at p < 0.05.
Findings: The findings of the study also indicated that the transcription of co-regulators is also link with the nutritional and temporal cues to the metabolism process by means of their association with circadian clock. The findings revealed that the hypothalamic circuits and its nutritional programming plays significant role in the regulation of energy homeostasis which is a major factor of obesity development in association with malnutrition in early life development.
Conclusion: Conclusively, it has been identified that the deficiency of nutrients during developmental period such as prenatal and postnatal is linked with the enhanced risk of different types of diseases in childhood as well as in adulthood.
Preventive Effect of Ethanol Extract of Red Spinach (Amaranthus tricolor L.) on Diet-induced Obese Zebrafish
Authors: Yuniarto Ari , Putri Aisyah Zavira , Selifiana Nita , Adnyana I. Ketut 
Published: February 2021

Background: Nowadays obesity recognized as chronic or non-communicable disease. Pathophysiology of obesity caused by an imbalance between energy intake and expenditure. Obesity was known to be a risk factor for the development of metabolic syndrome. The aim of this study is to evaluate activity of ethanol extract of red spinach (EERS) to prevent obesity in diet-induced zebrafish.
Materials and Methods: Acclimatization period for zebrafish was carried out for 2 weeks. After the acclimatization, zebrafish were divided into 6 groups (n = 10 in each group) such as normal group (negative control); obese group (positive control); standard drug (orlistat 4,5 µg/ml); EERS group (50 µg/ml); EERS group (100 µg/ml); and EERS group (200 µg/ml). During a period of 4 weeks, normal group received a standard diet and didn’t received EERS administration. Positive control group received Artemia. Treated group received Artemia and were combined by administration of EERS. To determine obesity criteria we calculated of zebrafish BMI.
Results: Based on BMI calculation, EERS 50 µg/ml, 100 µg/ml and 200 µg/ml showed a preventive effect on obesity compared to the positive control group. In addition, EERS 50 µg/ml was able to reduce BMI lower than the other extract groups.
Conclusion: It can be concluded that EERS 50 µg/ml has strength preventive effect on diet-induced obese zebrafish. This effect might be influenced by the presence of phytochemical compounds of extract such as flavonoid, saponins, and tannins.
Epistemologias marginalizadas: a questão racial no debate sociológico latino-americano
Authors: Da Silva Santos Oliveira Franciane , Pinheiro Barbosa Lia 
Published: February 2021

<p>Este artigo analisa o não lugar da questão racial e das epistemologias afrodiaspóricas no pensamento social e na sociologia latino-americana. Argumentamos que ainda é incipiente uma produção do conhecimento no campo sociológico que atente para o caráter marginal conferido às epistemologias africanas e afrodiaspóricas, resultado do racismo científico e da invisibilização dessas epistemologias. Problematizamos este debate enfatizando a necessidade histórica de uma produção sociológica que leve em consideração as epistemologias diaspóricas no processo de teorização sociológica e de seu papel na interpelação do suposto caráter universal de uma ciência eurocentrada e branca em detrimento de outros referentes epistêmicos do fazer sociológico.</p><p><strong>Palavras-chave: </strong>questão racial | epistemologias afrodiaspóricas | sociologia Latino-Americana | racismo epistêmico | descolonização científica.<strong></strong></p><p><strong><em> </em></strong></p><p><strong><em>Abstract:</em></strong></p><p><em>This article analyzes the </em><em>absence </em><em> of the racial question and African diasporic epistemologies in Latin American sociology and social thought. We argue that the production of knowledge that addresses the marginalization of African and African diasporic epistemologies in Latin American sociology is still incipient, as </em><em>the</em><em> result of scientific racism and the lack of visibility of these epistemologies. We further this debate by emphasizing the historical need for a sociological production that takes into account diasporic epistemologies in the process of sociological theoriz</em><em>ing</em><em>, and their role in questioning the supposedly universal character of an Eurocentric and white science, to the detriment of other epistemic referents of sociology.</em></p><p><strong><em>Keywords: </em></strong><em>racial question | afro-diasporic epistemologies | Latin American sociology | epistemic racism | scientific decolonization.</em></p>
A construção de personagens nos romances de Pepetela e a imaginação da nação angolana
Authors: Machado Carolina Bezerra 
Published: February 2021

<p>Este artigo tem como objetivo refletir sobre a construção de um imaginário acerca da nação angolana nas páginas dos romances do escritor Pepetela, mais especificamente em <em>A geração da utopia</em>, escrito entre 1991 e 1992. A partir de um debate sobre como os conceitos de nação e raça ainda permaneciam problemáticos no período pós-independência, podemos visualizar através do romance a tensão ainda existente na sociedade angolana nos anos 1990, parte de um debate político dentro do MPLA que influenciou diretamente o desenvolvimento de uma ideia de Estado e nação do país. Nesse sentido, os romances de Pepetela vão muito além de uma concepção de literatura pautada pela escrita ficcional, e passam a ser fundamentais para refletirmos sobre o imaginário angolano sob uma perspectiva histórica e política.</p><p><strong>Palavras-chave: </strong>Pepetela | Angola | Nação.</p><p> </p><p><strong><em>Abstract:</em></strong></p><p><em>This article analyzes the construction of a collective imaginary about Angola in the novels of the writer known as Pepetela</em><a href="file:///E:/AFROASIA%202020/AA%2062/revisado%20em%20WORD/AA_62_a%20Constru%C3%A7%C3%A3o%20(1).docx#_msocom_1">[1]</a> <em>, more specifically, the novel A Geração da Utopia, written between 1991 and 1992. By discussing how the concepts of nation and race remained problematic in the post-independence period, we can see through the novel the tension still existing in Angolan society at that time he 1990s and which was part of a political debate within the MPLA, </em><em>directly influencing the development of an idea of State and nation. In this sense, Pepetela's novels go far beyond a conception of literature as merely fictional writing, becoming fundamental for reflections on the Angolan imaginary from historical and political perspectives.</em></p><p><strong><em>Keywords: </em></strong><em>Pepetela | Angola | Nation.</em></p>
Entre cultura, solidariedade internacional e “mundo negro”: a negociação de sentidos na Présence Africaine (1955-56)
Authors: Reis Raissa Brescia dos 
Published: February 2021

<p>Este artigo parte das publicações da revista cultural francesa e senegalesa, <em>Présence Africaine</em>, propondo pensar como esta última procurou se posicionar, partindo do mundo intelectual, como interventora na imaginação e no fomento de soluções políticas e culturais para inserir novos participantes, principalmente africanos, no concerto internacional, em diálogo com bandeiras de solidariedade internacional/racial entre perspectivas locais e globais. Por meio da análise de debates, comunicações e mensagens retiradas das atas do Primeiro Congresso de Escritores e Artistas Negros, investiga-se como a revista se apropriou de novas linguagens e possibilidades políticas disponíveis e legitimadas no pós-Segunda Guerra Mundial, conciliando-as, não sem dificuldades, a discursos internacionalistas de movimentos políticos anteriores, como a <em>Négritude</em> e o pan-africanismo.</p><p><strong>Palavras-chave: </strong>presença africana | negritude | Pan-africanismo | História intelectual | Guerra Fria.</p><p> </p><strong><em>Abstract: </em></strong><p><em>This paper is based on publications of the French/Senegalese journal Présence Africaine and its intellectual stance in seeking to intervene in the design and dissemination of political/cultural solutions to include new participants (especially from Africa) in the international scene, in dialogue with the goal of international/racial solidarity goals at local and global levels. The paper examines debates, communications and messages from the annals of the First Congress of Black Writers and Artists in investigating how the journal appropriated new communicative strategies and political possibilities that emerged and were legitimized after World War II, in relation to the internationalizing discourses of previous political movements such as Négritude and Pan-Africanism.</em></p><p><strong>Keywords: </strong>présence africaine | négritude | Pan-Africanism | Intellectual history | Cold War.</p><p> </p>
O Atlântico enfermo e os agentes da saúde no período do tráfico ilegal
Authors: Kodama Kaori 
Published: February 2021

<p>Resenha de:<br />BARCIA, Manuel. <em>The Yellow Demon of Fever: Fighting Disease in the Nineteenth-Century Transatlantic Slave Trade</em>. New Heaven: Yale University Press, 2020. 269p.</p>
Sobre uma história social do trabalho e dos trabalhadores em Angola
Authors: Carvalho Ariane 
Published: February 2021

<p>Resenha de: <br />ALFAGALI, Crislayne Gloss Marão. <em>Ferreiros e fundidores da Ilamba. Uma história social da fabricação de ferro e da Real Fábrica de Nova Oeiras</em> <em>(Angola, segunda metade do século XVIII)</em>. Luanda: Fundação Dr. António Agostinho Neto, 2018. 472p.</p>
Cura, memória e política em Moçambique
Authors: Pereira Luena Nascimento Nunes 
Published: February 2021

<p>Resenha de:<br />SANTANA, Jacimara Souza. <em>Médicas-sacerdotisas: religiosidades ancestrais e contestação ao sul de Moçambique (c. 1927-1988)</em>. Campinas: Editora da Unicamp, 2018. 383p.</p>
Renamo: de agente do apartheid a organização política moçambicana
Authors: Conceição Juvenal De Carvalho 
Published: February 2021

<p>Resenha de:<br />CAHEN, Michel.<em>“Não somos bandidos”: a vida diária de uma guerrilha de direita: a Renamo na época do Acordo de Nkomati (1983-1985)</em>. Lisboa: ICS. Imprensa de Ciências Sociais, 2019. 398p.</p>
O racismo antinegro, a psicanálise e a subjetividade da nossa época no Brasil
Authors: Nicácio Jeferson De Jesus 
Published: February 2021

<p>Resenha de:<br />KON, Noemi: SILVA, Maria Lucia da; e ABDUL,<em> </em>Cristiane (orgs.). <em>O racismo e o negro no Brasil: questões para a psicanálise</em>. São Paulo: Perspectiva, 2017. 304p. </p>