bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircRNA-WNK2 Acts as a ceRNA for miR-328a-3p to Promote AANAT Expression in the Male Rat Pineal Gland
Authors: Zheng Yi , Wang Hao-Qi , Guo Hai-Xiang , Xie Heng-Li , Zhang Wei-Di , Han Dong-Xu , Jiang Hao , Yuan Bao , Zhang Jia-Bao 
Published: December 2021

Abstract
Noncoding RNAs (ncRNAs), including microRNAs (miRNAs) and circular RNAs (circRNAs), which are expressed with a daily rhythm in the rat pineal gland, are associated with the regulation of melatonin secretion and other biological functions. However, the mechanisms of these molecules in the rat pineal gland are not yet fully understood. In this study, we found that circR-WNK2 was highly expressed at night, which may be involved in the regulation of melatonin secretion through the competitive endogenous RNA (ceRNA) mechanism. By dual luciferase reporter, RNA pull-down, and fluorescence in situ hybridization (FISH) assays, we found that miR-328a-3p can target circR-WNK2 and the Aa-nat mRNA 3′UTR. Transfection experiments indicated that circR-WNK2 could competitively bind to miR-328a-3p, reduce miR-328a-3p expression, and promote Aa-nat gene expression and melatonin secretion. And by constructing a superior cervical ganglionectomy (SCGx) rat model, we found that ncRNAs expression in the pineal gland was regulated by signals from the suprachiasmatic nucleus. This finding supports the hypothesis that these noncoding RNAs may interact to shape the circadian rhythm through transcriptional processing in melatonin synthesis.
Contribution of protein–protein interactions to the endothelial-barrier-stabilizing function of KRIT1
Authors: Swamy Harsha , Glading Angela J. 
Published: December 2021

ABSTRACT
Krev-interaction trapped protein 1 (KRIT1) is an endothelial scaffold protein that promotes adherens junction (AJ) stability. The precise mechanism by which KRIT1 promotes barrier stabilization is unclear. We tested the ability of a panel of KRIT1 constructs containing mutations that inhibit Rap1 binding, ICAP1α binding, disrupt KRIT1's phosphotyrosine-binding (PTB) domain, or direct KRIT1 to the plasma membrane, either alone or in combination, to restore barrier function in KRIT1-deficient endothelial cells. We found that ablating the 192NPAY195 motif or disrupting the PTB domain was sufficient to restore AJ protein localization and barrier function to control levels, irrespective of the junctional localization of KRIT1 or Rap1 binding. The ability of our KRIT1 constructs to rescue AJ and barrier function in KRIT1-depleted endothelial cells correlated with decreased β1 integrin activity and maintenance of cortical actin fibers. Taken together, our findings indicate that Rap1 binding, ICAP1α binding and junctional localization are not required for the ability of KRIT1 to stabilize endothelial contacts, and suggest that the ability of KRIT1 to limit integrin activity could be involved in barrier stabilization.
The histone H4 lysine 20 demethylase DPY-21 regulates the dynamics of condensin DC binding
Authors: Breimann Laura , Morao Ana Karina , Kim Jun , Sebastian Jimenez David , Maryn Nina , Bikkasani Krishna , Carrozza Michael J. , Albritton Sarah E. , Kramer Maxwell , Street Lena Annika , Cerimi Kustrim , Schumann Vic-Fabienne , Bahry Ella , Preibisch Stephan , Woehler Andrew , Ercan Sevinç 
Published: December 2021

ABSTRACT
Condensin is a multi-subunit structural maintenance of chromosomes (SMC) complex that binds to and compacts chromosomes. Here, we addressed the regulation of condensin binding dynamics using Caenorhabditis elegans condensin DC, which represses X chromosomes in hermaphrodites for dosage compensation. We established fluorescence recovery after photobleaching (FRAP) using the SMC4 homolog DPY-27 and showed that a well-characterized ATPase mutation abolishes DPY-27 binding to X chromosomes. Next, we performed FRAP in the background of several chromatin modifier mutants that cause varying degrees of X chromosome derepression. The greatest effect was in a null mutant of the H4K20me2 demethylase DPY-21, where the mobile fraction of condensin DC reduced from ∼30% to 10%. In contrast, a catalytic mutant of dpy-21 did not regulate condensin DC mobility. Hi-C sequencing data from the dpy-21 null mutant showed little change compared to wild-type data, uncoupling Hi-C-measured long-range DNA contacts from transcriptional repression of the X chromosomes. Taken together, our results indicate that DPY-21 has a non-catalytic role in regulating the dynamics of condensin DC binding, which is important for transcription repression.
Indication to post-operative radiotherapy for oral cavity squamous cell carcinoma: what’s new in the depth of infiltration (DOI) era?
Authors: Alterio Daniela , De Berardinis Rita , Augugliaro Matteo , D’Urso Pasqualina , Volpe Stefania , Maffini Fausto , Bruschini Roberto , Marvaso Giulia , Riccio Stefano , Tagliabue Marta , Turturici Irene , Farneti Alessia , Calabrese Luca , Starzyńska Anna , Ferrari Annamaria , Zaffaroni Mattia , Jereczek-Fossa Barbara Alicja , Sanguineti Giuseppe , Ansarin Mohssen 
Published: December 2021

Objective:
The last edition of the American Joint Committee on Cancer (AJCC eighth) has introduced the depth of infiltration (DOI) as a new prognostic parameter in oral cavity squamous cell carcinomas (OCSCCs). The aim of this study is to analyze the impact of stage migration on the indication to post-operative radiotherapy (PORT).

Methods:
OCSCCs treated at two institutions between 2014 and 2019 were retrieved. As per the AJCC eighth, only pT3 primarily OCSCCs were considered; availability of the pathologic specimen was a further inclusion criterion. Risk factors considered for PORT were: pT3-pT4, nodal involvement, positive/close surgical margins, perineural and lymph vascular invasion.

Results:
149 patients staged as pT3 AJCC eighth were included. A four-fold increase in the number of patients staged as pT3 from the seventh to the eighth AJCC was found. Stage migration to pT3 was equally due to the downstaging from former pT4 (38%) and upstaging of former pT1-pT2 (35%). Considering the former pT1-pT2 53 patients, 13 (25%) had no risk factors for PORT other than DOI. Among 25 cases with former pT1-pT2 and negative lymph nodes, no additional risk factors were found in 11 (44%).

Conclusion:
90% of patients had at least one risk factor besides DOI and would have received PORT also according to the AJCC seventh; notably, of former pT1-pT2N0, half of them have been upstaged to pT3 in the current TNM classification. The role of PORT in this cohort of patients has not been clarified yet.

Advances in knowledge:
Other-than-DOI risk factors leading to PORT indication are highly prevalent in OCSSC patients classified as pT3 per the latest AJCC TNM staging system and should therefore be considered for a comprehensive oncological assessment.
Utilizing CT soft-tissue markers as a screening tool for acute invasive fungal sinusitis
Authors: John Deepa Susan , Shyam Karthik , Andrew Dhilip , Cicilet Soumya , Deepalam Saikanth Reddy 
Published: December 2021

Objectives:
Acute invasive fungal sinusitis (AIFS) is a rapidly progressive disease, whose delayed identification results in poor outcomes, especially in immunocompromised individuals. A surge in of AIFS in the wake of the COVID-19 pandemic has lent additional morbidity and mortality to an already precarious clinical scenario. Early detection of AIFS in individuals who are symptomatic/ at risk can allow early therapy, enabling better patient outcomes. Our study aims to determine optimal soft-tissue markers on CT for the early detection of AIFS.

Methods:
In this case–control study, 142 patients with equal distribution of subjects were chosen based on histopathological diagnosis of AIFS; and their non-contrast CT scans were retrospectively assessed to determine the diagnostic utility of specific soft-tissue markers that would enable diagnosis of AIFS.

Results:
A total of nine markers with adequate sensitivity and specificity were identified, including pterygopalatine and sphenopalatine fossae, inferior orbital fissure and nasolacrimal duct involvement, premaxillary thickening, retro-antral and orbital stranding, and infratemporal muscle oedema. It was determined that the combined occurrence of any three out of nine markers was 91.5% sensitive and 95.9% specific for diagnosis of AIFS (p &lt; 0.005).

Conclusion:
Early, accurate detection of AIFS in predisposed individuals is possible with identification of soft-tissue markers on NECT, enabling early intervention.

Advances in knowledge:
Being the aggressive disease that it is, AIFS may be managed early if the index of suspicion is held high via CT imaging; which our diagnostic checklist aims at enabling.
The quantitative impact of joint peer review with a specialist radiologist in head and neck cancer radiotherapy planning
Authors: Chiu Kevin , Hoskin Peter , Gupta Amit , Butt Roeum , Terparia Samsara , Codd Louise , Tsang Yatman , Bhudia Jyotsna , Killen Helen , Kane Clare , Ghoshray Subhadip , Lemon Catherine , Megias Daniel 
Published: December 2021

Objectives:
Radiologist input in peer review of head and neck radiotherapy has been introduced as a routine departmental approach. The aim was to evaluate this practice and to quantitatively analyse the changes made.

Methods:
Patients treated with radical-dose radiotherapy between August and November 2020 were reviewed. The incidence of major and minor changes, as defined by The Royal College of Radiologists guidance, was prospectively recorded. The amended radiotherapy volumes were compared with the original volumes using Jaccard Index (JI) to assess conformity; Geographical Miss Index (GMI) for undercontouring; and Hausdorff Distance (HD) between the volumes.

Results:
In total, 73 out of 87 (84%) patients were discussed. Changes were recommended in 38 (52%) patients: 30 had ≥1 major change, eight had minor changes only. There were 99 amended volumes: The overall median JI, GMI and HD was 0.91 (interquartile range [IQR]=0.80–0.97), 0.06 (IQR = 0.02–0.18) and 0.42 cm (IQR = 0.20–1.17 cm), respectively. The nodal gross-tumour-volume (GTVn) and therapeutic high-dose nodal clinical-target-volume (CTVn) had the biggest magnitude of changes: The median JI, GMI and HD of GTVn was 0.89 (IQR = 0.44–0.95), 0.11 (IQR = 0.05–0.51), 3.71 cm (IQR = 0.31–6.93 cm); high-dose CTVn was 0.78 (IQR = 0.59–0.90), 0.20 (IQR = 0.07–0.31) and 3.28 cm (IQR = 1.22–6.18 cm), respectively. There was no observed difference in the quantitative indices of the 85 ‘major’ and 14 ‘minor’ volumes (p = 0.5).

Conclusions:
Routine head and neck radiologist input in radiotherapy peer review is feasible and can help avoid gross error in contouring.

Advances in knowledge:
The major and minor classifications may benefit from differentiation with quantitative indices but requires correlation from clinical outcomes.
Strychnine poisoning due to traditional Chinese medicine: a case series
Authors: Tong Hok-Fung , Chan Candace Yim , Ng Sau-Wah , Mak Tony Wing-Lai 
Published: December 2021

Background: Strychnine poisoning is rare but possibly fatal. The most reported sources of strychnine poisoning include rodenticides and adulterated street heroin. Here we report a case series of an unusual cause of strychnine poisoning – Strychni semen, a herb known as “maqianzi” in traditional Chinese medicine (TCM). Methods: All cases of strychnine poisoning confirmed by the Hospital Authority Toxicology Reference Laboratory (HATRL, the highest-level clinical toxicology laboratory in Hong Kong) between May 2005 and May 2018 were reviewed. Results: Twelve cases of strychnine poisoning were recorded, and Strychni semen was the exclusive source. Ten (83%) patients presented with muscle spasms, and four (33%) developed typical conscious convulsions. The poisoning was severe in two (17%) patients, moderate in three (25%) and mild in eight (58%). No case fatality was recorded. Three (25%) patients were TCM practitioners and two (17%) were laymen who bought the herb themselves without a proper prescription. Conclusion: The practice of TCM is becoming popular in different parts of the world amid the COVID-19 pandemic. The spectrum of clinical features of strychnine poisoning secondary to Strychni semen are similar to those arising from different origins. Eliciting a history of TCM use, apart from exposure to rodenticides and drugs of abuse, may allow timely diagnosis in patients with compatible clinical features. Enhancement of TCM safety could minimize the hazard.
Zygmunt Miłkowski (Tomasz Teodor Jeż) a Narodowa Demokracja
Authors: Zaleska Ilona 
Published: December 2021

Zygmunt Miłkowski to jedna najważniejszych postaci polskiej emigracji demokratycznej, mogąca się poszczycić imponującym dorobkiem pisarskim oraz doświadczeniem działalności politycznej. Za przełomowy w jego politycznym życiorysie można uznać 1887 rok, w którym ukazała się broszura „Rzecz o obronie czynnej i Skarbie Narodowym”, a także powstała niepodległościowa Liga Polska. Miłkowski był współtwórcą organizacji oraz autorem podstawy programowej. W ramach Ligi Miłkowski nawiązał współpracę z przyszłymi prominentnymi politykami ruchu narodowo-demokratycznego. Współpraca ta była kontynuowana również po rozwiązaniu Ligi Polskiej i powstaniu w jej miejsce Ligi Narodowej, której ton nadawali trzej działacze: Roman Dmowski, Zygmunt Balicki i Jan Ludwik Popławski. Z Ligi Narodowej wyrosła jedna z najważniejszych polskich formacji politycznych - Narodowa Demokracja. Miłkowski od początku wspierał działalność Ligi wierząc, że będzie ona realizowała program niepodległościowy. Reorientacja polityczna Narodowej Demokracji w kierunku polityki rozumnej ugody z Rosją dokonująca się w pierwszym dziesięcioleciu XX w., nigdy nie zyskała jego akceptacji. Zaważyła na dalszych relacjach z endecją i sprawiła, że Miłkowski publicznie potępił dawnych uczniów i politycznych sojuszników.
Portugalska Rewolucja Goździków w 1974 roku. Przyczyny-przebieg-następstwa
Authors: Kubiak Krzysztof 
Published: December 2021

Streszczenie 25 kwietnia 1974 w Lizbonie rozpoczął się wojskowy zamach stanu wymierzony w autorytarne państwo. Odniósł błyskawiczny sukces. Przejęcie władzy przez wojsko rozpoczęło ciąg niezwykle turbulentnych wydarzeń o kluczowym znaczeniu dla przyszłości państwa. Za zakończenie owego okresu uznać można 25 listopada 1975 roku. Wtedy to również wojsko udaremniło kolejną próbę zamachu organizowaną przez oficerów o radykalnie lewicowych poglądach. Celem artykułu jest dokonanie rekonstrukcji przyczyn, które doprowadziły do zamachu oraz omówienie przebiegu wydarzeń między kwietniem 1974 roku a listopadem roku następnego. Okres ten określany jest w artykule mianem Rewolucji goździków. Autor posłużył się metodą studium przypadku, w wariancie ukierunkowanym nie tylko na opis, ale również na zaprezentowanie kontekstu badanego zjawiska. Podłużono się opracowaniami w języku portugalskimi angielskim, a także wyborem portugalskich dokumentów i aktów prawnych. AbstractOn April 25 1974 the military coup d'état aimed at the authoritarian state started in Lisboan. The rebels achieved an instant success. The takeover of power by the military started a series of extremely turbulent events of key importance for the future of the state. The end of this period can be considered November 25 1975. It was also then that the military foiled another attempted conducted by officers with radical leftist views. The aim of the article is to reconstruct the causes that led to the coup d'état in April 1974, to discuss the course of events between April 1974 and November of the following year. The period is referred to in this article as the Revolution cloves. The paper presents the consequences of these events for the further history of the Portugal. The author used the case study method in a variant aimed not only at description but also at presenting the context of the phenomenon under study. The studies in Portuguese and English, as well as the selection of Portuguese documents and legal acts, were extended.
Orfeusz, śpiewak mityczny – ani tracki ani pieryjski. Polemika z recenzją Krzysztofa Nawotki
Authors: Mojsik Tomasz 
Published: December 2021

Streszczenie: Artykuł jest polemiką z recenzją Krzysztofa Nawotki („Klio” 2020, t. 54, s. 199–208) i skupia się na kwestiach źródłoznawczych, metodologii, etniczności antycznej i badaniach nad mitem. Abstract: The article is a polemic with a review by Krzysztof Nawotka (“Klio” 2020, vol. 54, pp. 199–208) and focuses on the issues of source studies, methodology, ancien ethnicity and research on myth.
Market Efficient Portfolios in a Systemic Economy
Authors: Awiszus Kerstin , Capponi Agostino , Weber Stefan 
Published: December 2021

Diversification vs. Diversity – How is the Efficiency of Markets Affected? Prices aggregate information that is dispersed in the economy; they thereby facilitate the allocation of scarce resources. Inefficiencies may arise from deviations of market prices from their fundamental values. In “Market Efficient Portfolios in a Systemic Economy”, Awiszus, Capponi, and Weber investigate the impact of the trade-off between diversity and diversification on inefficiencies in secondary markets due to asset illiquidity and leverage constraints of financial institutions. The authors identify two key determining factors. These are the systemic significance of the banks and the statistical properties of the fundamental asset shocks. Systemic significance is driven by the banks’ target leverage, their trading strategies, and the illiquidity characteristics of the assets. The paper demonstrates that portfolio diversification is typically not efficient. In fact, efficient portfolio holdings may strongly deviate from this standard paradigm, especially if the banks have similar characteristics.
The Differential Impact of Intrafirm Collaboration and Technological Network Centrality on Employees’ Likelihood of Leaving the Firm
Authors: Tzabbar Daniel , Cirillo Bruno , Breschi Stefano 
Published: December 2021

How does an employee’s centrality in intrafirm research and development activities affect the employee’s propensity for outward mobility? Does this proclivity vary by the type of employment the employee seeks: moving to other firms versus founding a new venture? We maintain that, to answer these questions, we must distinguish between an employee’s centrality in the intrafirm collaboration network and the employee’s centrality in the intrafirm technological recombination network. We utilize the curricula vitae and patent data of corporate inventors at a leading semiconductor company between 1993 and 2012 to test our hypotheses. Contrary to prevailing views, our competing risk model indicates that corporate inventors who are central in the intrafirm collaboration and technological network and, thus, have the most opportunities are less likely to leave the current employer. However, when considering external employment opportunities, their preferences vary. Collaboration-central individuals are more likely to start a new venture than to move to another employer. Their skill in developing interpersonal relationships enables them to attract the tangible and intangible resources needed in a new firm. In contrast, inventors whose technological expertise is central to the firm’s technology recombination network are more likely to move to another employer than to start a new venture. In an established firm, they can leverage their technological know-how using the resources that a new venture would lack. Our theory highlights the trade-offs in employees’ attempts to take advantage of their internal and external value based on their position within the firm’s collaboration and technological networks. Funding: The authors thank LeBow College of Business, SKEMA Business School, and Bocconi University for their financial support. Supplemental Material: The online appendix is available at https://doi.org/10.1287/orsc.2021.1535 .
REVISTA CONTRAPONTO: UMA VISÃO HUMANIZADA DO MUNICÍPIO DO RIO DE JANEIRO E SEUS PERSONAGENS
Authors: Barreto Ivana 
Published: December 2021

Resumo: Os meios de comunicação de massa contribuem para o processo de reciclagem de estereótipos e preconceitos e, como resultado, muitos deles se tornam duradouros. Nesse sentido, a preocupação com a questão dos direitos humanos é inquestionável, bem como sua discussão e produção de pesquisas no âmbito universitário. Desse modo, alunos do Curso de Jornalismo da Universidade Federal Rural do Rio de Janeiro (UFRRJ) produziram uma revista para se opor ao conteúdo predominante da mídia hegemônica, apresentando visões mais humanizadas sobre o município do Rio de Janeiro e alguns de seus personagens. O objetivo foi realizar uma cobertura reflexiva, contribuindo para o questionamento dos estereótipos mencionados. A linha editorial foi baseada nos direitos humanos e na ética. Devido à falta de patrocínio e dificuldades de impressão, o produto tornou-se disponível na internet e está sendo divulgado em congressos. A metodologia incluiu pesquisas sobre os temas elencados, trabalho de campo e entrevistas. Periodicamente, foram realizadas reuniões para avaliar a produção e sua vinculação com a linha editorial. Quanto aos resultados, há o prêmio na XXIII Expocom Sudeste e a receptividade na própria universidade. Este artigo, portanto, é um relato de experiência da Revista Contraponto.Palavras-chave: Direitos Humanos. Ética. Jornalismo. Revista. Contraponto.Abstract : The mass media contributes to the process of recycling stereotypes and prejudices and as result many of them become enduring. In this sense, the concern with the issue of Human Rights is undisputed as wellas its discussion and production of research in the university level. Thus, students from the Journalism Course of UFRRJ produced a magazine in order to oppose the prevailing contente of the hegemonic media, presenting more humanized visions about Rio de Janeiro and some of its characters. The goal was to carry out reflective coverage, contributing to the questioning of the stereotypes. The editorial line was based on the humanrights and ethics. Due to the lack of sponsorship and printing difficulties, the product became available on the internet and is being also publicized in congresses. The methodology included research on the themes listed, field work and interviews. Periodically, meetings were held to evaluate the production and its link to the editorial line. As regards the results, there are the XXIII Expocom Sudeste Award and receptivity in the university it self. This article, therefore, is an experience report of the Revista Contraponto.Keywords: Human rights. Ethics. Jornalism. Magazine. Contraponto.