bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multilineage-differentiating stress-enduring cells alleviate atopic dermatitis-associated behaviors in mice
Authors: Fei WenDi , Wu JunLin , Gao MengDie , Wang Qian , Zhao Ya Yu , Shan ChunLi , Shen Yu , Chen Gang 
Published: December 2021

AbstractBackgroundPruritus is a recurring, long-lasting skin disease with few effective treatments. Many patients have unsatisfactory responses to currently available antipruritic treatments, and effective therapeutics are urgently needed to relieve symptoms. A previous study reported that mesenchymal stem cell (MSC)-mediated immune regulation could be used to treat skin inflammatory diseases. Multilineage-differentiating stress-enduring (Muse) cells are a new type of pluripotent stem cell that may also have the potential to treat inflammatory skin diseases.MethodsMuse cells were isolated from human bone marrow-derived MSCs (BMSCs) via the 8-h longterm trypsin incubation (LTT) method. Repeated use of 2,4-dinitrofluorobenzene (DNFB) induced atopic dermatitis (AD) in a mouse model. Immunofluorescence, behavior recording, and image analysis were used to evaluate the therapeutic effect of subcutaneous Muse cell injection. Real-time quantitative polymerase chain reaction (qPCR) was used to measure the expression of inflammatory factors. In vitro, wound healing and cell proliferation experiments were used to examine the effect of Muse cell supernatant on keratinocytes.ResultsOur results showed that subcutaneous injection of Muse cells after AD model induction significantly alleviated scratching behavior in mice. The evaluation of dermatitis and photos of damaged skin on the back of the neck revealed that Muse cells reduced dermatitis, playing an active role in healing the damaged skin. The activation of spinal glial cells and scratching behavior were also reduced by Muse cell injection. In addition, we also showed that the expression levels of the inflammatory factors interleukin (IL)-6, IL-17α, and IL-33 in both the spinal cord and skin were suppressed by Muse cells. Furthermore, Muse cells not only exerted anti-inflammatory effects on lipopolysaccharide (LPS)-induced human HaCat cells but also promoted wound healing and keratinocyte proliferation.ConclusionsIn vivo, Muse cells could alleviate scratching symptoms, reduce epidermal inflammation, and promote wound healing. In vitro, Muse cells could also promote the migration and proliferation of keratinocytes. In summary, Muse cells may become a new therapeutic agent for the treatment of AD.
Induction of meiosis by embryonic gonadal somatic cells differentiated from pluripotent stem cells
Authors: Wang Haiying , Liu Linlin , Liu Chang , Wang Lingling , Chen Jiyu , Wang Huasong , Heng Dai , Zeng Ming , Liu Chun , Zhou Zhongcheng , Ye Xiaoying , Wan Yajuan , Li Huiyu , Liu Lin 
Published: December 2021

Abstract
Background
Depletion of oocytes leads to ovarian aging-associated infertility, endocrine disruption and related diseases. Excitingly, unlimited oocytes can be generated by differentiation of primordial germ cell like cells (PGCLCs) from pluripotent stem cells. Nevertheless, development of oocytes and follicles from PGCLCs relies on developmentally matched gonadal somatic cells, only available from E12.5 embryos in mice. It is therefore imperative to achieve an in vitro source of E12.5 gonadal somatic cells.

Methods
We explored to identify small molecules, which can induce female embryonic stem cells (ESCs) into gonadal somatic cell like cells.

Results
Using RNA-sequencing, we identified signaling pathways highly upregulated in E12.5_gonadal somatic cells (E12.5_GSCs). Through searching for the activators of these pathways, we identified small-molecule compounds Vitamin C (Vc) and AM580 in combination (V580) for inducing differentiation of female embryonic stem cells (ESCs) into E12.5_GSC-like cells (E12.5_GSCLCs). After V580 treatment for 6 days and sorted by a surface marker CD63, the cell population yielded a transcriptome profile similar to that of E12.5_GSCs, which promoted meiosis progression and folliculogenesis of primordial germ cells. This approach will contribute to the study of germ cell and follicle development and oocyte production and have implications in potentially treating female infertility.

Conclusion
ESCs can be induced into embryonic gonadal somatic cell like cells by small molecules.
Sex differences in the immune response to acute COVID-19 respiratory tract infection
Authors: Qi Shaohua , Ngwa Conelius , Morales Scheihing Diego A. , Al Mamun Abdullah , Ahnstedt Hilda W. , Finger Carson E. , Colpo Gabriela Delevati , Sharmeen Romana , Kim Youngran , Choi HuiMahn A. , McCullough Louise D. , Liu Fudong 
Published: December 2021

Abstract
Background
Sex differences in COVID-19 are increasingly recognized globally. Although infection rates are similar between the sexes, men have more severe illness. The mechanism underlying these sex differences is unknown, but a differential immune response to COVID-19 has been implicated in several recent studies. However, how sex differences shape the immune response to COVID-19 remains understudied.

Methods
We collected demographics and blood samples from over 600 hospitalized patients diagnosed with COVID-19 from May 24th 2020 to April 28th, 2021. These patients were divided into two cohorts: Cohort 1 was further classified into three groups based on the severity of the disease (mild, moderate and severe); Cohort 2 patients were longitudinally followed at three time points from hospital admission (1 day, 7 days, and 14 days). MultiPlex and conventional ELISA were used to examine inflammatory mediator levels in the plasma in both cohorts. Flow cytometry was conducted to examine leukocyte responses in Cohort 2.

Results
There were more COVID+ males in the total cohort, and the mortality rate was higher in males vs. females. More male patients were seen in most age groups (in 10-year increments), and in most ethnic groups. Males with severe disease had significantly higher levels of pro-inflammatory cytokines (IL-6, IL-8, MCP-1) than females; levels of IL-8, GRO, sCD40L, MIP-1β, MCP-1 were also significantly higher in severe vs. mild or control patients in males but not in females. Females had significantly higher anti-inflammatory cytokine IL-10 levels at 14 days compared to males, and the level of IL-10 significantly increased in moderate vs. the control group in females but not in males. At 7 days and 14 days, males had significantly more circulating neutrophils and monocytes than females; however, B cell numbers were significantly higher in females vs. males.

Conclusion
Sex differences exist in hospitalized patients with acute COVID-19 respiratory tract infection. Exacerbated inflammatory responses were seen in male vs. female patients, even when matched for disease severity. Males appear to have a more robust innate immune response, and females mount a stronger adaptive immune response to COVID-19 respiratory tract infection.
GADD34-mediated dephosphorylation of eIF2α facilitates pseudorabies virus replication by maintaining de novo protein synthesis
Authors: Zhu Ting , Jiang Xueli , Xin Hangkuo , Zheng Xiaohui , Xue Xiaonuan , Chen Ji-Long , Qi Baomin 
Published: December 2021

AbstractViruses have evolved multiple strategies to manipulate their host’s translational machinery for the synthesis of viral proteins. A common viral target is the alpha subunit of eukaryotic initiation factor 2 (eIF2α). In this study, we show that global protein synthesis was increased but the eIF2α phosphorylation level was markedly decreased in porcine kidney 15 (PK15) cells infected with pseudorabies virus (PRV), a swine herpesvirus. An increase in the eIF2α phosphorylation level by salubrinal treatment or transfection of constructs expressing wild-type eIF2α or an eIF2α phosphomimetic [eIF2α(S51D)] attenuated global protein synthesis and suppressed PRV replication. To explore the mechanism involved in the inhibition of eIF2α phosphorylation during PRV infection, we examined the phosphorylation status of protein kinase R-like endoplasmic reticulum kinase (PERK) and double-stranded RNA-dependent protein kinase R (PKR), two kinases that regulate eIF2α phosphorylation during infection with numerous viruses. We found that the level of neither phosphorylated (p)-PERK nor p-PKR was altered in PRV-infected cells or the lungs of infected mice. However, the expression of growth arrest and DNA damage-inducible protein 34 (GADD34), which promotes eIF2α dephosphorylation by recruiting protein phosphatase 1 (PP1), was significantly induced both in vivo and in vitro. Knockdown of GADD34 and inhibition of PP1 activity by okadaic acid treatment led to increased eIF2α phosphorylation but significantly suppressed global protein synthesis and inhibited PRV replication. Collectively, these results demonstrated that PRV induces GADD34 expression to promote eIF2α dephosphorylation, thereby maintaining de novo protein synthesis and facilitating viral replication.
Identification of a new amino acid mutation in the HN protein of NDV involved in pathogenicity
Authors: Chen Xi , Jia Yanqing , Wei Ning , Ye Chao , Hao Huafang , Xiao Sa , Wang Xinglong , Liu Haijin , Yang Zengqi 
Published: December 2021

AbstractThe fusion (F) and haemagglutinin-neuraminidase (HN) proteins of Newcastle disease virus (NDV) are viral entry proteins and are recognized as the major virulence determinants. Previously, a lentogenic NDV virus (CE16) was derived from a mesogenic strain (CI10) through sequential passages in chick embryos. Whole-genome sequence analysis revealed that the two homologous strains shared the same F protein but differed in HN with two amino acid (aa) substitutions (A215G and T430A). To elucidate the molecular reasons for virulence attenuation, two original plasmids (HN-CI10 and HN-CE16) and two single-point mutants (G215A and A430T) reverse-mutated from HN-CE16 were constructed to analyse the known biological functions of HN. The results showed that the A430T substitution significantly weakened the haemadsorption (HAd) activity, increased the neuraminidase (NA) activity, improved the fusion-promoting activity, and enhanced the cleavage-promoting activity of HN-CE16. However, G215A failed to induce obvious functional changes. Therefore, the aa residue HN430 may play a key role in determining virulence. To test this hypothesis, further studies on A430T were conducted through reverse genetics using an infectious cDNA clone. At the viral level, the A430T-mutated virus showed dramatic promotion of viral plaque formation, propagation, and pathogenicity in vitro and in vivo. This study demonstrates a new virulence site associated with HN protein functions, viral propagation, and pathogenicity. All these findings could lay a foundation for illuminating the molecular mechanism of NDV virulence.
An integrative model of patients’ perceived value of healthcare service quality in North Cyprus
Authors: Sanıl Mert , Eminer Fehiman 
Published: December 2021

Abstract
Background
Improving healthcare quality has become an essential objective for all health institutions worldwide to address the need to improve services, manage costs and satisfy patient expectations about the quality of care. As health is one of the leading service sectors of the North Cyprus economy, analysing patients’ perceived value of healthcare service quality is crucial. In this research, a comparative analysis of existing models revealed affordability, acceptability and accessibility as the leading modern service quality indicators affecting patients’ perceived value of healthcare service quality. The quality of services is a leading factor impacting business competition and retention dictated by the current market. This study aimed to investigate the factors that influence patient perceptions of healthcare service quality in North Cyprus.

Methods
A self-administered questionnaire was carried out among 388 patients of public and private hospitals in North Cyprus, and the data were analysed using partial least squares-structural equation modelling.

Results
Empirical results highlight that the acceptability of healthcare services is a prerequisite for perceiving a high value of service quality. The affordability and accessibility of services, respectively, were less effective. Results concerning mediating effects confirm that acceptability could fully mediate the relationship between affordability and perceived value and could partially mediate the impact of accessibility on the perceived quality of healthcare services.

Conclusion
This study contributes to healthcare theory and practice by developing a conceptual framework to provide policymakers and managers with a practical understanding of factors that affect healthcare service quality.
The need for nutritional assessment and interventions based on the prognostic nutritional index for patients with femoral fractures: a retrospective study
Authors: He Miao , Fan Qinghong , Zhu Yuhang , Liu Dexing , Liu Xingxing , Xu Shan , Peng Jiachen , Zhu Zhaoqiong 
Published: December 2021

Abstract
Background
The incidence of adverse perioperative outcomes in surgery for femoral fractures is high and associated with malnutrition. Here, we identified independent factors and assessed the predictive value of the prognostic nutritional index (PNI) for perioperative adverse outcomes in patients with femoral fractures.

Methods
This retrospective study included 343 patients who underwent surgery for a single femur fracture. Demographic characteristics, surgery and anaesthesia records and blood test results at admission, 1 day postoperatively and before discharge were evaluated using logistic regression analysis. The discriminatory ability of the independent factors was assessed using the receiver operating characteristic curve analysis, and DeLong’s test was used to compare the area under the curve (AUC).

Results
Overall, 159 patients (46.4%) experienced adverse perioperative outcomes. Amongst these, 123 (35.9%) had lower limb vein thrombus, 68 (19.8%) had hospital-acquired pneumonia, 6 (1.7%) were transferred to the postoperative intensive care unit, 4 (1.2%) had pulmonary embolism, 3 (0.9%) died during hospitalisation and 9 (2.6%) had other adverse outcomes, including incision disunion, renal and liver function impairment, acute heart failure, acute cerebral infarction and stress gastroenteritis. The PNI at admission, age, postoperative hospital stay, time to admission, hypertension, combined injures and surgery type were independent factors for adverse perioperative outcomes. Based on the AUC (PNI at admission: 0.772 [0.723–0.821], P < 0.001; age: 0.678 [0.622–0.734], P < 0.001; postoperative hospital stay: 0.608 [0.548–0.668], P = 0.001; time to admission: 0.585 [0.525–0.646], P = 0.006), the PNI at admission had optimal discrimination ability, indicating its superiority over other independent factors (age vs. PNI at admission, P = 0.002; postoperative hospital stay vs. PNI at admission, P < 0.001; time to admission vs. PNI at admission, P < 0.001).

Conclusions
Patients with femoral fractures require a nutritional assessment and appropriate nutritional intervention at admission, and that the PNI value at admission may be a good nutritional assessment indicator.
Effect of plant growth-promoting rhizobacterial treatment on growth and physiological characteristics of Triticum aestivum L. under salt stress
Authors: Lee Dong Gun , Lee Ji Min , Choi Chang Geun , Lee Hojoung , Moon Jun Cheol , Chung Namhyun 
Published: December 2021

AbstractSalinity stress is a serious abiotic stress that affects crop quality and production. Rhizospheric microbes have immense potential in synthesizing and releasing various compounds that regulate plant growth and soil physicochemical properties. The aim of the present study was to evaluate the efficacy of indole-3-acetic acid (IAA)-producing rhizobacteria as biofertilizers under salt stress. Among the isolated strains from various soil samples, Bacillus megaterium strain PN89 with multifarious plant growth-promoting traits was selected and used as a monoculture and co-culture with two other standard strains. The plant promoting activity was evaluated using the paper towel method and pot test to observe the effects on the early stage and vegetative growth of wheat (Triticum aestivum L.). The treatment using PGPR strain presented noticeable but varying effects on plant growth under salt stress, that is, PGPR treatment often displayed a significant increase in germination percentage, root and shoot length, and other growth parameters of wheat compared to those in the non-inoculated control. Thus, these results suggest that B. megaterium PN89 can be applied as a bio-fertilizer to alleviate salt stress in T. aestivum.
Mass Concrete Placement of the Offshore Wind Turbine Foundation: A Statistical Approach to Optimize the Use of Fly Ash and Silica Fume
Authors: Van Tran Mien , Chau Vinh Ngoc 
Published: December 2021

AbstractThe experimental program investigated concrete with a large amount of fly ash (FA) with silica fume (SF) to replace Portland cement on the results of semi-adiabatic test, compressive strength test, and the rapid chloride permeability test (RCPT). The replacement ratios of cement by a combination of FA and SF were 30%, 35%, and 40% by mass. The percentages of SF to replace cement were 0%, 4%, and 8% by mass. Three different water-to-binder ratios (W/B) of 0.34, 0.36, and 0.38 were also investigated. Multiple linear regression was applied to construct the predicted equations (models) for the semi-adiabatic temperature rise test and the compressive strength test. Models were assessed statistically and were used to solve the concrete mixture design optimization problems. The mixture with W/B of 0.36, 31% FA, and 5% SF was found to optimally satisfy the multi-objective problem: 28-day compressive strength of 50 MPa, low heat of hydration, and very low chloride penetrability classification. Field test on the actual wind turbine foundation of the optimal mixture revealed the maximum temperature rise was 74.8 °C and the maximum temperature differential was 21.9 °C.
Investigating the utility of a high-temperature Thellier-style paleointensity experimental protocol
Authors: Grappone J. Michael , Russell James M. , Biggin Andrew J. 
Published: December 2021

AbstractHigh-quality data are vital to the research field of paleointensity, which has long suffered from poor-quality and/or sparse data. Previous paleointensity work has established that repeatedly heating specimens increases the opportunity for thermochemical alteration to decrease the reliability of paleointensity data. In addition, recent work has shown that repeatedly heating specimens in paleointensity experiments can also exaggerate the effects of non-ideal, non-single domain grains. Arai plots resulting from paleointensity experiments containing such grains are often curvilinear (two-slope) across most of the specimen’s unblocking temperature spectrum, except in the temperature range nearest to the grains’ Curie temperature. This study tests the following strategy to mitigate these effects: that of performing a Thellier paleointensity experiment using fewer temperature steps that are also concentrated at higher temperatures. For this purpose, we use natural specimens with well-constrained rock magnetic data from the Hawaiian Scientific Observation Hole 1 (SOH1) drill core in paleointensity experiments with starting temperatures ranging from 200 °C to 500+ °C. Those experiments that focused in on the portion of the unblocking temperature spectrum nearest the Curie temperature of the specimen (HiTeCT) had an exceptionally low success rate, whereas those with initial temperatures at comparatively moderate temperatures (200–400 °C) had high success rates (~ 70%). Thermochemical alteration was minimized with a start temperature of 400 °C, but the curvature of the Arai plots had no clear dependance on start temperature. We conclude herein that increasing the start temperature can help avoid the effects of low-temperature alterations. Additionally, we found that the approach of focusing in on the highest temperature range is not a feasible one to apply in paleointensity experiments, in general, and consider this likely to be a result of a lack of intermediate-temperature checks for alteration and insufficient independence of temperature steps.
Graphical abstract
Application of an improved clustering approach on GPS height time series at CMONOC stations in Southwestern China
Authors: Wu Shuguang , Li Zhao , Li Houpu , Nie Guigen , Liu Jingnan , He Yuefan 
Published: December 2021

AbstractThe hydrological, geological and meteorological conditions in southwestern China are relatively complex, so that the land surface deformation presents various features. Using 58 Crustal Movement Observation Network of China (CMONOC) stations across four provinces in Southwestern China, we adopt an improved clustering algorithm to classify 49 stations into 12 clusters with different similarity levels. Our results show that the average annual signals of GPS stations within each cluster have strong consistency, while obvious differences exist among the 12 clusters, indicating that clustering algorithm helps to describe surface deformation features more accurately in regions with complex conditions. We then combine other earth observation techniques, such as the Gravity Recovery and Climate Experiment (GRACE) satellite datasets and surface loading models (SLM), and observe that GPS, GRACE and SLM have strong correlation in their monthly displacement series at GPS stations. After excluding non-clustered stations according to our previous clustering results, the correlation coefficients of GPS/GRACE and GPS/SLM are enhanced. Also, the RMS reduction rates of GPS coordinate time series have been improved after deducting displacements obtained from GRACE and SLM, thus the clustering algorithm proves to be effective in improving the consistency of three techniques in joint detection of surface deformation. Moreover, the vertical displacements of four riverside GPS stations in the Three Gorges Reservoir (TGR) area show significant negative correlation with water level of TGR, hence we conclude that the Three Gorges Dam (TGD) may directly affect the consistency of GPS annual signals of its upstream and downstream GPS stations.
Graphical Abstract