bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shaping modern human skull through epigenetic, transcriptional and post-transcriptional regulation of the RUNX2 master bone gene
Authors: Di Pietro Lorena , Barba Marta , Palacios Daniela , Tiberio Federica , Prampolini Chiara , Baranzini Mirko , Parolini Ornella , Arcovito Alessandro , Lattanzi Wanda 
Published: October 2021

AbstractRUNX2 encodes the master bone transcription factor driving skeletal development in vertebrates, and playing a specific role in craniofacial and skull morphogenesis. The anatomically modern human (AMH) features sequence changes in the RUNX2 locus compared with archaic hominins’ species. We aimed to understand how these changes may have contributed to human skull globularization occurred in recent evolution. We compared in silico AMH and archaic hominins’ genomes, and used mesenchymal stromal cells isolated from skull sutures of craniosynostosis patients for in vitro functional assays. We detected 459 and 470 nucleotide changes in noncoding regions of the AMH RUNX2 locus, compared with the Neandertal and Denisovan genomes, respectively. Three nucleotide changes in the proximal promoter were predicted to alter the binding of the zinc finger protein Znf263 and long-distance interactions with other cis-regulatory regions. By surface plasmon resonance, we selected nucleotide substitutions in the 3’UTRs able to affect miRNA binding affinity. Specifically, miR-3150a-3p and miR-6785-5p expression inversely correlated with RUNX2 expression during in vitro osteogenic differentiation. The expression of two long non-coding RNAs, AL096865.1 and RUNX2-AS1, within the same locus, was modulated during in vitro osteogenic differentiation and correlated with the expression of specific RUNX2 isoforms. Our data suggest that RUNX2 may have undergone adaptive phenotypic evolution caused by epigenetic and post-transcriptional regulatory mechanisms, which may explain the delayed suture fusion leading to the present-day globular skull shape.
Foetal growth, birth transition, enteral nutrition and brain light scattering
Authors: Iwata Osuke , Iwata Sachiko , Kurata Tsuyoshi , Tsuda Kennosuke , Kawase Koya , Kinoshita Masahiro , Lin Yung-Chieh , Saikusa Mamoru , Araki Yuko , Takashima Sachio , Oda Motoki , Ohmae Etsuko , Saitoh Shiji 
Published: October 2021

AbstractIf the brain structure is assessed at neonatal intensive care units, covert clinical events related with subtle brain injury might be identified. The reduced scattering coefficient of near-infrared light (μS’) obtained using time-resolved near-infrared spectroscopy from the forehead of infants is associated with gestational age, body weight and Apgar scores, presumably reflecting subtle changes of the brain related to foetal growth and birth transition. One hundred twenty-eight preterm and term infants were studied to test whether μS’ obtained from the head at term-equivalent age is associated with foetal growth, birth transition and nutritional status after birth, which are key independent variables of developmental outcomes. As potential independent variables of μS’, birth weight, Apgar scores, age at full enteral feeding and post-conceptional age at the study were assessed to represent foetal growth, birth transition and nutritional status after birth. Subsequently, higher μS’ values were associated with higher Apgar scores (p = 0.003) and earlier establishment of enteral feeding (p < 0.001). The scattering property of near-infrared light within the neonatal brain might reflect changes associated with birth transition and nutritional status thereafter, which might be used as a non-invasive biomarker to identify covert independent variables of brain injury in preterm infants.
Investigation of the effects of pretreatment on the elemental composition of ash derived from selected Nigerian lignocellulosic biomass
Authors: Awoyale Adeolu A. , Lokhat David , Okete Patrick 
Published: October 2021

AbstractLignocellulosic biomass is an important source of renewable energy and a potential replacement for fossil fuels. In this work, the X-ray fluorescence (XRF) method was used to analyze the elemental composition of raw and pretreated lignocellulosic biomass of cassava peels, corn cobs, rice husks, sugarcane bagasse, yam peels, and mixtures of cassava peels and yam peels, corn cobs and rice husks and all five biomass samples combined. The influence of particle size on elemental properties was investigated by screening the selected biomass into two size fractions, of an average of 300 and 435 µm, respectively. The total concentration of Mg, Al, Si, P, S, Cl, Ca, Ti, Cr, Mn, Fe, Co, Cu, Zn, Sn, Ni, Br, Mo, Ba, Hg, and Pb were determined for each of the biomass samples before and after the different pretreatments adopted in this study. From the results of the analysis, there was a significant reduction in the concentration of calcium in all the analyzed biomass after the alkaline pretreatment with rice husks biomass having the lowest concentration of 66 ppm after the alkaline pretreatment. The sulfur content of the acid pretreated biomass increased considerably which is likely due to the sulfuric acid used for the acid pretreatment. The fact that a mixture of biomass feedstock affects the properties of the biomass after pretreatment was validated in the mixed biomass of cassava peels and yam peels biomass as an example. The concentration of Mg in the mixed biomass was 1441 ppm but was 200 ppm and 353 ppm in individual cassava peels and yam peels respectively. The results of this study demonstrated that pretreated mixtures of biomass have varied elemental compositions, which could be an important factor affecting downstream processes, especially if a hybrid feedstock is used in a large-scale application.
Lipidomic response of the entomopathogenic fungus Beauveria bassiana to pyrethroids
Authors: Litwin Anna , Bernat Przemysław , Nowak Monika , Słaba Mirosława , Różalska Sylwia 
Published: October 2021

AbstractPyrethroids are chemical insecticides that are widely used to control pests. Entomopathogenic fungi are considered environmentally safe alternatives to these compounds. Pyrethroids and entomopathogenic fungi not only co-exist in the environment but can also be applied together in pest control. They are often found in contact with each other, and thus, it seems important to understand their interactions at the cellular level. In this study, we analyzed whether pyrethroids could influence the phospholipid profile of Beauveria bassiana and whether membrane changes are one of the mechanisms by which these fungi adapt to unfavorable environmental conditions. The results of our study revealed that pyrethroids changed the phospholipid profile and increased the cell membrane permeability of B. bassiana, which enabled them to enter and accumulate within the fungal cells, resulting in oxidative stress. Pyrethroids influenced the amount of neutral lipids, caused a decrease in sodium content, and also temporarily lowered the level of the secondary metabolite oosporein in the studied fungi. These findings indicate that the effect of pyrethroids on entomopathogenic fungi may be more complex than originally thought and that lipidomic studies can aid in fully understanding the influence of these chemicals on the mentioned group of fungi.
High-pressure thermal conductivity and compressional velocity of NaCl in B1 and B2 phase
Authors: Hsieh Wen-Pin 
Published: October 2021

AbstractSodium chloride (NaCl) is an important, commonly used pressure medium and pressure calibrant in diamond-anvil cell (DAC) experiments. Its thermal conductivity at high pressure–temperature (P–T) conditions is a critical parameter to model heat conduction and temperature distribution within an NaCl-loaded DAC. Here we couple ultrafast optical pump-probe methods with the DAC to study thermal conductivity and compressional velocity of NaCl in B1 and B2 phase to 66 GPa at room temperature. Using an externally-heated DAC, we further show that thermal conductivity of NaCl-B1 phase follows a typical T−1 dependence. The high P–T thermal conductivity of NaCl enables us to confirm the validity of Leibfried-Schlömann equation, a commonly used model for the P–T dependence of thermal conductivity, over a large compression range (~ 35% volume compression in NaCl-B1 phase, followed by ~ 20% compression in the polymorphic B2 phase). The compressional velocities of NaCl-B1 and B2 phase both scale approximately linearly with density, indicating the applicability of Birch’s law to NaCl within the density range we study. Our findings offer critical insights into the dominant physical mechanism of phonon transport in NaCl, as well as important data that significantly enhance the accuracy of modeling the spatiotemporal evolution of temperature within an NaCl-loaded DAC.
Torque capacity of multidisc wet clutch with reference to friction occurrence on its spline connections
Authors: Bąk Marcin 
Published: October 2021

AbstractIn this article developed mathematical model that includes friction occurrence on spline connections is presented. The work also contains results of experimental research on torque capacity of multidisc wet clutch. These results are expressed as a function of contact pressure for different number of friction surfaces. Due to increased interest in research concerning multidisc wet clutches it is essential to determine impact of friction on fit connections on transmitted torque. Analytical calculations that include both known loss coefficient and assumed lack of friction on fit connections are compared to results of experiments. The paper contains detailed description of test stand and methodology of experiment. As a result of conducted tests it was found that correction coefficients known from literature are highly inaccurate. Measured values of torque indicate that transmitted torque reach significantly higher values. It was also revealed that after slippage appeared, the pressure plate usually moved in the direction of exerted clamping force, but movement in reversed direction also took place for some experiments. While movement corresponding to clamping force reached ca. 0.08 mm, in opposite direction amounted to 0.02 mm. Furthermore, studies presented that lapping of adjacent friction surfaces greatly affects differences between respective results obtained for a specific experiment.
Dysbiosis and structural disruption of the respiratory microbiota in COVID-19 patients with severe and fatal outcomes
Authors: Hernández-Terán Alejandra , Mejía-Nepomuceno Fidencio , Herrera María Teresa , Barreto Omar , García Emma , Castillejos Manuel , Boukadida Celia , Matias-Florentino Margarita , Rincón-Rubio Alma , Avila-Rios Santiago , Mújica-Sánchez Mario , Serna-Muñoz Ricardo , Becerril-Vargas Eduardo , Guadarrama-Pérez Cristobal , Ahumada-Topete Víctor Hugo , Rodríguez-Llamazares Sebastián , Martínez-Orozco José Arturo , Salas-Hernández Jorge , Pérez-Padilla Rogelio , Vázquez-Pérez Joel Armando 
Published: October 2021

AbstractThe COVID-19 outbreak has caused over three million deaths worldwide. Understanding the pathology of the disease and the factors that drive severe and fatal clinical outcomes is of special relevance. Studying the role of the respiratory microbiota in COVID-19 is especially important as the respiratory microbiota is known to interact with the host immune system, contributing to clinical outcomes in chronic and acute respiratory diseases. Here, we characterized the microbiota in the respiratory tract of patients with mild, severe, or fatal COVID-19, and compared it to healthy controls and patients with non-COVID-19-pneumonia. We comparatively studied the microbial composition, diversity, and microbiota structure between the study groups and correlated the results with clinical data. We found differences in the microbial composition for COVID-19 patients, healthy controls, and non-COVID-19 pneumonia controls. In particular, we detected a high number of potentially opportunistic pathogens associated with severe and fatal levels of the disease. Also, we found higher levels of dysbiosis in the respiratory microbiota of patients with COVID-19 compared to the healthy controls. In addition, we detected differences in diversity structure between the microbiota of patients with mild, severe, and fatal COVID-19, as well as the presence of specific bacteria that correlated with clinical variables associated with increased risk of mortality. In summary, our results demonstrate that increased dysbiosis of the respiratory tract microbiota in patients with COVID-19 along with a continuous loss of microbial complexity structure found in mild to fatal COVID-19 cases may potentially alter clinical outcomes in patients. Taken together, our findings identify the respiratory microbiota as a factor potentially associated with the severity of COVID-19.
Safety and efficacy of prophylactic tirofiban infusion for acute intracranial intraprocedural stent thrombosis
Authors: Sun Lili , Zhang Jinping , Song Yun , Zhao Wei , Zheng Meimei , Zhang Jun , Yin Hao , Wang Wei , Meng Yao , Han Ju 
Published: October 2021

AbstractPeriprocedural antithrombotic management with glycoprotein IIb/IIIa inhibitors (GPI) for intracranial artery stenting is still controversial. We sought to assess the safety and efficacy of prophylactic tirofiban infusion for acute intracranial intraprocedural stent thrombosis in routine clinical practice. From January 2013 to December 2019, consecutive patients treated with endovascular stenting for symptomatic intracranial atherosclerotic stenosis (ICAS) were identified and dichotomized by whether tirofiban was used. The efficacy and safety outcomes were compared by propensity score matching. A total of 160 consecutive patients in the tirofiban group and 177 patients in the non-tirofiban group were enrolled. Propensity score matching analysis selected 236 matched patients. One acute intraprocedural stent thrombosis (AIST) occurred in patients receiving prophylactic tirofiban, while 8 in the non-tirofiban group. The incidence of AIST in the tirofiban group was significantly lower than that in the non-tirofiban group (0.8% vs 6.8%, P = 0.039). The periprocedural ischemic events (8.5% vs 5.1%, P = 0.424), periprocedural intracranial hemorrhage (4.2% vs 0.8%, P = 0.219) and 30-day total mortality (3.4% vs 0%, P = 0.125) were not statistically different between the two groups. Compared with conventional stenting angioplasty without tirofiban, tirofiban prophylactic infusion can lower the incidence of AIST, without increasing the risk of periprocedural intracranial hemorrhage and 30-day total mortality. However, there is no superiority in reducing periprocedural ischemic events. The current study adds more important insights to the available clinical evidence on the use of tirofiban during stenting of ICAS.
Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces
Authors: Hallinan Jazmine P. , Doyle Lindsey A. , Shen Betty W. , Gewe Mesfin M. , Takushi Brittany , Kennedy Madison A. , Friend Della , Roberts James M. , Bradley Philip , Stoddard Barry L. 
Published: October 2021

AbstractCircular tandem repeat proteins (‘cTRPs’) are de novo designed protein scaffolds (in this and prior studies, based on antiparallel two-helix bundles) that contain repeated protein sequences and structural motifs and form closed circular structures. They can display significant stability and solubility, a wide range of sizes, and are useful as protein display particles for biotechnology applications. However, cTRPs also demonstrate inefficient self-assembly from smaller subunits. In this study, we describe a new generation of cTRPs, with longer repeats and increased interaction surfaces, which enhanced the self-assembly of two significantly different sizes of homotrimeric constructs. Finally, we demonstrated functionalization of these constructs with (1) a hexameric array of peptide-binding SH2 domains, and (2) a trimeric array of anti-SARS CoV-2 VHH domains. The latter proved capable of sub-nanomolar binding affinities towards the viral receptor binding domain and potent viral neutralization function.
Non-conventional octameric structure of C-phycocyanin
Authors: Minato Takuo , Teramoto Takamasa , Adachi Naruhiko , Hung Nguyen Khac , Yamada Kaho , Kawasaki Masato , Akutsu Masato , Moriya Toshio , Senda Toshiya , Ogo Seiji , Kakuta Yoshimitsu , Yoon Ki-Seok 
Published: October 2021

AbstractC-phycocyanin (CPC), a blue pigment protein, is an indispensable component of giant phycobilisomes, which are light-harvesting antenna complexes in cyanobacteria that transfer energy efficiently to photosystems I and II. X-ray crystallographic and electron microscopy (EM) analyses have revealed the structure of CPC to be a closed toroidal hexamer by assembling two trimers. In this study, the structural characterization of non-conventional octameric CPC is reported for the first time. Analyses of the crystal and cryogenic EM structures of the native CPC from filamentous thermophilic cyanobacterium Thermoleptolyngbya sp. O–77 unexpectedly illustrated the coexistence of conventional hexamer and novel octamer. In addition, an unusual dimeric state, observed via analytical ultracentrifugation, was postulated to be a key intermediate structure in the assemble of the previously unobserved octamer. These observations provide new insights into the assembly processes of CPCs and the mechanism of energy transfer in the light-harvesting complexes.
The Galactic Faraday rotation sky 2020
Authors: Hutschenreuter S. , Anderson C. S. , Betti S. , Bower G. C. , Brown J.-A. , Brüggen M. , Carretti E. , Clarke T. , Clegg A. , Costa A. , Croft S. , Van Eck C. , Gaensler B. M. , de Gasperin F. , Haverkorn M. , Heald G. , Hull C. L. H. , Inoue M. , Johnston-Hollitt M. , Kaczmarek J. , Law C. , Ma Y. K. , MacMahon D. , Mao S. A. , Riseley C. , Roy S. , Shanahan R. , Shimwell T. , Stil J. , Sobey C. , O’Sullivan S. P. , Tasse C. , Vacca V. , Vernstrom T. , Williams P. K. G. , Wright M. , Enßlin T. A. 
Published: October 2021

Aims. This work provides an update to existing reconstructions of the Galactic Faraday rotation sky by processing almost all Faraday rotation data sets available at the end of the year 2020. Observations of extra-Galactic sources in recent years have further illuminated the previously underconstrained southern celestial sky, as well as parts of the inner disc of the Milky Way, along with other regions. This has culminated in an all-sky data set of 55 190 data points, thereby comprising a significant expansion on the 41 330 used in previous works. At the same time, this novelty makes an updated separation of the Galactic component a promising enterprise. The increased source density allows us to present our results in a resolution of about 1.3 × 10−2 deg2 (46.8 arcmin2), which is a twofold increase compared to previous works.
Methods. As for previous Faraday rotation sky reconstructions, this work is based on information field theory, namely, a Bayesian inference scheme for field-like quantities that handles noisy and incomplete data.
Results. In contrast to previous reconstructions, we find a significantly thinner and pronounced Galactic disc with small-scale structures exceeding values of several thousand rad m−2. The improvements can mainly be attributed to the new catalog of Faraday data, but are also supported by advances in correlation structure modeling within numerical information field theory. We also provide a detailed discussion on the statistical properties of the Faraday rotation sky and we investigate correlations with other data sets.
Millimetre-wave laboratory study of glycinamide and a search for it with ALMA towards Sagittarius B2(N)
Authors: Kisiel Z. , Kolesniková L. , Belloche A. , Guillemin J.-C. , Pszczółkowski L. , Alonso E. R. , Garrod R. T. , Białkowska-Jaworska E. , León I. , Müller H. S. P. , Menten K. M. , Alonso J. L. 
Published: October 2021

Context. Glycinamide (NH2CH2C(O)NH2) is considered to be one of the possible precursors of the simplest amino acid, glycine. Its only rotational spectrum reported so far has been in the centimetre-wave region on a laser-ablation generated supersonic expansion sample.
Aims. The aim of this work is to extend the laboratory spectrum of glycinamide to the millimetre (mm) wave region to support searches for this molecule in the interstellar medium and to perform the first check for its presence in the high-mass star forming region Sagittarius B2(N).
Methods. Glycinamide was synthesised chemically and was studied with broadband rotational spectroscopy in the 90–329 GHz region with the sample in slow flow at 50°C. Tunnelling across a low-energy barrier between two symmetry equivalent configurations of the molecule resulted in splitting of each vibrational state and many perturbations in associated rotational energy levels, requiring careful coupled state fits for each vibrational doublet. We searched for emission of glycinamide in the imaging spectral line survey ReMoCA performed with the Atacama Large Millimetre/submillimetre Array towards Sgr B2(N). The astronomical spectra were analysed under the assumption of local thermodynamic equilibrium.
Results. We report the first analysis of the mm-wave rotational spectrum of glycinamide, resulting in fitting – to experimental measurement accuracy – of over 1200 assigned and measured transition frequencies for the ground-state tunnelling doublet and of many lines for tunnelling doublets for two singly excited vibrational states. We also determine the precise vibrational separation in each doublet. We did not detect emission from glycinamide in the hot molecular core Sgr B2(N1S). We derived a column density upper limit of 1.5 × 1016 cm−2, which implies that glycinamide is at least seven times less abundant than aminoacetonitrile and 1.8 times less abundant than urea in this source. A comparison with results of astrochemical kinetics models for species related to glycinamide suggests that its abundance may be at least one order of magnitude below the upper limit obtained towards Sgr B2(N1S). This means that glycinamide emission in this source likely lies well below the spectral confusion limit in the frequency range covered by the ReMoCA survey.
Conclusions. Thanks to the spectroscopic data provided by this study, the search for glycinamide in the interstellar medium can continue on a firm basis. Targetting sources with a lower level of spectral confusion, such as the Galactic Center shocked region G+0.693-0.027, may be a promising avenue.
Two New Cytotoxic Maytansinoids Targeting Tubulin from Trewia nudiflora
Authors: Lei Chun , Li Ya-Nan , Li Jia-Nan , Zhou Yu-Bo , Cui Ming-Jun , Fu Kai-Cong , Li Jia , Hou Ai-Jun 
Published: October 2021

AbstractTwo new maytansinoids, N-methyltreflorine (1) and methyltrewiasine (2), were isolated from the dried fruits of Trewia nudiflora, together with three known congeners (3 – 5). Their structures were elucidated by spectroscopic methods, and the absolute configuration of 1 and 2 was determined by X-ray crystallographic analysis. Compounds 1 – 5 exhibited strong cytotoxicity against human tumor cell lines, including HeLa, MV-4 – 11, and MCF-7, with IC50 values ranging from 0.12 to 11 nM. Compounds 1 and 4 also showed inhibitory activity against the MCF-7/ADR cell line with IC50 values of 13 and 28 nM, respectively. Compounds 1 and 2 significantly inhibited tubulin polymerization in vitro with IC50 values of 3.6 and 3.2 µM, respectively.