bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-cultural Adaptation and Validation of the Arabic Version of the Harrus Hip Score
Authors: Alshaygy Ibrahim , Alageel Musab Abdulaziz , Aljurayyan Abdulaziz , Griffen Anthony , Arafah Orfan , Nasse Ahmed bin , Alsudairi Abdulaziz , Alsubaie Mohammed , Almousa Yasmeen , Alyousef Nada , Aljasser Fawzi 
Published: April 2021

Abstract
Background: The goal of this study was to translate the Harris Hip (HHS) questionnaire into the Arabic language with cross-cultural adaptation to include and benefit Arabic speaking communities as it is the most widely used instrument for disease-specific hip joint evaluation and measurement of total hip arthroplasty outcome. Methods: This questionnaire was translated following A clear and user-friendly guideline protocol. The Cronbach’s alpha was used to assess the reliability and internal consistency of the items of HHS. Additionally, The constructive validity of HHS was evaluated against the 36-Item Short Form Survey (SF 36). Results: A total of 100 participants were included in this study, of which 30 participants were re-evaluated for reliability testing. Cronbach’s alpha of the total score of Arabic HHS is 0.528 and after the standardization, it changed to 0.742 which is within the recommended range (0.7-0.9). Lastly, The correlation between HHS and SF 36 was r=0.71 (p<0.001) which represents a strong correlation between Arabic HHS and SF-36. Conclusion: Based on the results, we believe that the Arabic HHS can be used by clinicians, researchers, and patients to evaluate and report hip pathologies and total hip arthroplasty treatment efficacy.
Patient's Perceived Empathy Can Predict Doctor-patient Relationship in Medical Interaction in China
Authors: HAN Caixia , WU Qing , LIU CHENCHEN , WANG Pei 
Published: April 2021

Abstract
ObjectiveTo explore the relationship among patient perception of doctors' empathy, doctor-patient communication, and doctor-patient relationship.MethodsFrom November 2019 to April 2020, we used the CARE scale, the SEGUE framework and the PDRQ-15 scale to survey 3,039 patients from 14 provinces in China, ranging in age from 18 to 92 years old.Results1. The age of the patient, the level and type of hospital, the length of consultation, and the presence or absence of verbal and physical conflict have significant differences in the scores of perceived empathy; 2. There was a significantly positive correlation among patient's perceived empathy, doctor-patient communication, and the doctor-patient relationship (r = 0.65 ~ 0.75, p < 0.001). 3. The patient's perception of doctor empathy can not only directly predict doctor-patient relationship (β = 0.75, p < 0.001), but also influence doctor-patient relationship through the mediating effect of doctor-patient communication (β = 0.56, p < 0.001). Besides, the scores of CARE can effectively explain the variation of each stage of doctor-patient communication skills (R2 = 0.28 ~ 0.37) and the satisfaction (52%) and approachability (54%) of the doctor-patient relationship scale.ConclusionThe age of the patient, the type and level of the hospital, and the consultation time affected perceived empathy. Doctor-patient communication plays a partial mediating effect in the influence of empathy on the doctor-patient relationship.Practice implicationsMedical institutes should improve the medical environment and carry out humanistic education and patient-centered communication skills training for doctors. These approaches will enhance patients' perceived empathy and therefore improve the doctor-patient relationship.
Early response of methanogenic archaea to H2 as evaluated by metagenomics and metatranscriptomics
Authors: Kakuk Balázs , Wirth Roland , Maróti Gergely , Márk Szuhaj , Rakhely Gábor , Laczi Krisztián , Kovács Kornél Lajos , Bagi Zoltán 
Published: April 2021

Abstract
Background.

The detailed molecular machinery of the complex microbiological cell factory of biogas/biomethane production is not fully understood. One of the main puzzling process control elements is the formation, consumption and regulatory role of hydrogen (H2). Reduction of carbon dioxide (CO2) by H2 is rate limiting factor in methanogenesis, but the community intends to keep H2 concentration low in order to maintain the redox balance of the overall system. H2 metabolism in methanogens becomes increasingly important in the Power-to-Gas renewable energy conversion and storage technologies.
Results.

The early response of the mixed mesophilic microbial community to H2 gas injection was investigated with the goal of uncovering the first responses of the microbial community in the CH4 formation and CO2 mitigation Power-to-Gas process. The overall microbial composition changes, following a 10 min H2 injection by excessive bubbling of H2 through the reactor, was investigated via metagenome and metatranscriptome sequencing. The overall composition and taxonomic abundance of the biogas producing anaerobic community did not change appreciably two hours after the H2 treatment, indicating that this time period was too short to display differences in the proliferation of the members of the microbial community. There was, however, a substantial increase in the expression of genes related to hydrogenotrophic methanogenesis of certain groups of Archaea. H2 injection also altered the metabolism of a number of microbes belonging in the kingdom Bacteria. The importance of syntrophic cross-kingdom interactions in H2 metabolism and the effects on the related Power-to-Gas process are discussed.
Conclusion

s. External H2 regulates the functional activity of certain Bacteria and Archaea. Mixed communities are recommended for the large scale Power-to-Gas process rather than single hydrogenotrophic methanogen strains. Fast and reproducible response from the microbial community can be exploited in turn-off and turn-on of the Power-to-Gas microbial cell factories.
Ultrasonic-Assisted Drilling of Cortical and Cancellous Bone in a Comparative Point of View
Authors: Pourgiv Sousan , Jamshidi Nima , Mohammadi Aminollah , Mosavar Alireza 
Published: April 2021

Abstract
Background: A potential method in drilling of bone is ultrasonic-assisted drilling. In addition, during the drilling of bone, which is common in clinical surgeries, excessive heat generation and drilling force may lead to damages in bone tissue, and thus to failure of implants and fixation screws or delay in healing process. The aim of this study was to appraise efficiency of ultrasonic-assisted drilling in comparison to conventional drilling.Methods: In addition to investigating drilling force and temperature elevation, their effects on arising osteonecrosis and micro-cracks were explored in ultrasonic-assisted and conventional drilling through histopathologic assessment and microscopic imaging. In this regard, three drilling speeds and two drilling feed-rates were considered as drilling variables in the in-vitro experiments. Moreover, numerical modeling gave an insight into temperature distribution during drilling process in the both methods and compared three different vibration amplitudes. Results: Although temperature elevations were lower in the conventional drilling, the ultrasonic-assisted drilling had lesser drilling forces. Furthermore, the latter method had smaller osteonecrosis regions, and did not have micro-cracks in cortical bone and destructions in structure of cancellous bone.Conclusions: The ultrasonic-assisted drilling, which caused lesser damages to the bone tissue in both cortical and cancellous bone, was more comparatively advantageous.
Influence of clear cell carcinoma on the post-hepatectomy prognosis of patients with hepatocellular carcinoma
Authors: Deng Yongheng , Zhu Shaoliang , Qi Lunan , Chen Zushun , Ma Liang 
Published: April 2021

Abstract
Background To evaluate whether the presence of clear cell carcinoma in patients with hepatocellular carcinoma affects their prognosis after hepatectomy.Methods Data were retrospectively analyzed for 470 patients with hepatocellular carcinoma, of whom 239 also had clear cell carcinoma. All patients were treated by hepatectomy at our hospital between October 2007 and March 2020. Overall survival (OS) and disease-free survival (DFS) were compared between patients with or without clear cell carcinoma. A Cox proportional hazards model was used to identify factors independently associated with survival. A nomogram was formulated to predict long-term prognosis of patients with hepatocellular carcinoma containing clear cell carcinoma following hepatectomy.Results The presence of clear cell carcinoma was associated with significantly higher DFS (P = 0.007) and OS (P < 0.001). Multivariate analysis identified the following factors as significantly associated with DFS: alpha-fetoprotein level (AFP), tumor size, liver cirrhosis, satellite nodules and portal vein tumor thrombosis (PVTT). The following factors were significantly associated with OS: tumor size, satellite nodules, capsule formation and Ki-67. A nomogram incorporating these independent prognostic factors showed a concordance index of 0.660 for predicting DFS and an index of 0.730 for predicting OS.Conclusion Clear cell carcinoma is associated with better post-resection prognosis of patients with hepatocellular carcinoma. Tumor size and satellite nodules may be independent predictors of OS and DFS.
Clinical Whole-Exome Sequencing Analysis Reveals a Novel Missense COL11A1 Mutation Resulting in an 18-Week Iranian Male Aborted Fetus with Fibrochondrogenesis 1: A Case Report
Authors: Mirtavoos-Mahyari Hanifeh , Ajami Sanaz , Mehrtash Amirhosein , Marashiyan Seyedeh Mahya , Bahreini Farbod , Sheikhy Kambiz , Ghanbari Sogol , Ardeshirdavani Amin 
Published: April 2021

Abstract
BackgroundFibrochondrogenesis 1, an autosomal recessive syndrome, is an infrequent and rare disease, causing short-limbed skeletal dysplasia. This syndrome is clinically characterized and distinguished by a small nose and anteverted bares, flat midface, shortened long bones, and a protuberant abdomen. Mutations in the gene encoding the α1 chain of type XI collagen (COL11A1) are seen to be the main cause of this disease.Case PresentationWe present an 18-week Iranian male aborted fetus with Fibrochondrogenesis 1 from consanguineous parents. Whole-exome sequencing (WES) revealed a novel missense variant from G to A in exon 45 of 68 in the COL11A1 gene (NM_080629.2: c.3440G>A, [p.G1147E, g.103404625]). The mutation was confirmed by Sanger sequencing and further, MutationTaster predicted this variant to be disease-causing.Conclusion﻿Bioinformatic analysis suggests that this variant is highly conserved in both nucleotide and protein levels, suggesting that it has an important function in the proper role of COL11A1 protein. In-silico analysis suggests that this mutation alters the COL11A1 protein structure through a Glycine to Glutamic acid substitution. This is a novel mutation and a rare variant as this variant is not reported in gmomAD, ExAC, or 1000 genome databases.To the best of the authors’ knowledge, this is the first study to report a novel pathogenic mutation in COL11A1 in association with Fibrochondrogenesis 1. Therefore, we suggest that WES can be used as a robust method to achieve rapid diagnosis and identification of pathogenic and novel mutations in patients.
Clinical Application and Technical Details of Cook Zenith Devices modification to treat Urgent and Elective Complex Aortic Aneurysms
Authors: Manunga Jesse , Jordan Lia , Mirza Aleem K. , Teng Xiaoyi , Skeik Nedaa , Eisenmenger Laura 
Published: April 2021

Abstract
Purpose: To describe technical details of modifying four different Cook Zenith devices to treat complex aortic aneurysms.Material: In the first three cases, the modification process involved complete stent graft deployment on a sterile back table. Fenestrations were created using an ophthalmologic cautery and reinforced with a radiopaque snare using a double-armed 4-0 Ethibond locking suture based on measurements obtained on centerline of flow. In each instance, a nitinol wire was withdrawn and redirected through and through the fabric and used as a constraining wire. In the fourth patient, modification involved partial stent graft deployment and creation of additional two fenestrations to accommodate renal arteries. The devices are resheathed and implanted in the standard fashion.Results: Four patients underwent exclusion of their aneurysms, including thoracoabdominal aneurysms (n=2), a contained ruptured juxtarenal aneurysm (n=1), and a ruptured failed previous endovascular repair (n=1). Fifteen fenestrations were successfully bridged with Atrium iCAST stent grafts. Average graft modification time, operative time, contrast volume, radiation dose, estimated blood loss, and hospital length of stay were 89 minutes, 155.25 minutes, 58.8 mL, 2451 mGy, 175 mL, and 4.3 days, respectively. One patient required a secondary intervention to treat a type Ib endoleak. During an average follow-up of 25 months, aneurysm sacs progressively shrank without additional intervention.Conclusion: Physician-modified fenestrated/branched endografts are a safe alternative to custom made devices, especially in urgent cases and should be part of the armamentarium of any complex aortic program.
Time makes histone H3 modifications drift
Authors: Hillje Roman , Luzi Lucilla , Amatori Stefano , Fanelli Mirco , Pelicci Pier Giuseppe , Giorgio Marco 
Published: April 2021

Abstract
To disclose the epigenetic drift of time passing, we determined the genome-wide distributions of mono- and tri-methylated lysine 4 and acetylated and tri-methylated lysine 27 of histone H3 in the livers of healthy 3, 6 and 12 months old C57BL/6 mice. The comparison of different age profiles of histone H3 marks revealed global redistribution of histone H3 modifications with time, in particular in intergenic regions and near transcription start sites, as well as altered correlation between the profiles of different histone modifications. Moreover, feeding mice with caloric restriction diet, a treatment known to retard aging, preserved younger state of histone H3 in these genomic regions.
Association between triglyceride glucose index and abdominal aortic calcification
Authors: Liu Yihai , Wu Mingyue , Xu Biao , Kang Lina 
Published: April 2021

Abstract
Background

The triglyceride glucose (TyG) index has been regarded as a simple surrogate marker of insulin resistance. However, few studies have investigated the relationship between the TyG index and abdominal aortic calcification (AAC), an independent predictor of atherosclerotic vascular diseases and mortality.
Methods

A total of 1486 participants with comleted AAC records from the National Health and Nutrition Examination Survey (NHANES) 2013–2014 were enrolled. Baseline variables and abdominal dual-energy X-ray absorptiometry (DXA) data were collected. The TyG index was calculated as ln[triglycerides (mg/dL)×glucose (mg/dL)/2], and the presence of AAC was defined as AAC score > 0.
Results

The participants were 58.8 ± 12.2 year old and 48.6% (722/1486) were male. TyG level was positively correlated with the presence of AAC (β = 1.37, [1.15–1.63]; p < 0.001). The highest quartile of TyG was associated with 1.83-fold (odds ratio [OR]: 1.31–2.58; p < 0.001) risk of AAC compared with the lowest quantile. After adjusted for lifestyles, comorbidities and calcium metabolism factors, higher TyG level was significantly and linearly associated with higher AAC prevalence. The multivariate-adjusted OR and 95% confidence interval (CI) were 2.12 (1.05–4.35; p = 0.038) for participants within the highest quantile and 1.60 (1.07–2.41; p = 0.022) per one-unit incresement. Subgroup analysis showed that the association between TyG and AAC was still significant across groups except for age > 60.
Conclusions

Higher TyG index was significantly associated with the higher presence of AAC, which could be a marker of AAC.
Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps
Authors: Ghorbani Mahla , Azghandi Marjan , Khayyami Reza , Baharara Javad , Kerachian Mohammad Amin 
Published: April 2021

Abstract
Background: The incidence of colorectal cancer (CRC) has increased during recent years in Iran and other developing countries. Clinical studies suggest that essential folate dietary intake and moderate deficiency of methylenetetrahydrofolate reductase (MTHFR) may protect and reduce the risk of CRC.The aim of the present study was to investigate the clinical significance of C677T polymorphism within MTHFR gene and its correlation with the serum folate and Vit B12 in the Iranian population suffering from CRC.Methods: Blood samples were taken from 1017 Iranian individuals (517 cases and 500 controls) who were referred for colonoscopy. TaqMan probe assay was performed for C677T MTHFR polymorphism. Sera were fractionated from the blood samples of 43 patients and controls and folate and Vit B12 concentrations were measured by a monobind kit. The correlation of MTHFR polymorphisms and folate/vitamin-B12 with CRC risk were analyzed.Results: In the current study, we found the frequency of three different genotypes of MTHFR polymorphism in the Iranian population i.e., CC, CT and TT, to be 51.31, 26.73, 21.96 and 61, 32.2, 6.8 in case and control groups, respectively. The homozygote genotype of MTHFR rs1801133 polymorphism is associated with increased risk of CRC by 3.68, 1.42 and 3.74-fold in codominant, dominant and recessive models respectively (p value< 0.01). Our study revealed that there was no significant difference between the amount of folate and Vit B12 in case and control groups (p value> 0.05).Conclusions: This study revealed that there was no significant difference between the amount of folate and Vit B12 in the case and control groups. Furthermore, our results demonstrated a higher risk association for 677TT and 677TT+C677T genotypes of MTHFR compared with 677CC carriers among CRC patients.
Dorobek kanonistyki uprawianej na Katolickim Uniwersytecie Lubelskim w przedmiocie materialnego prawa małżeńskiego w okresie międzywojnia (1918-1939)
Authors: Dzierżon Ginter 
Published: April 2021

W prezentowanym opracowaniu przedmiotem uwagi Autora stała się problematyka dorobku kanonistyki uprawianej na Katolickim Uniwersytecie Lubelskim w przedmiocie materialnego prawa małżeńskiego w okresie międzywojnia (1918-1939). Badając spuściznę materialną na którą złożyły się opublikowane monografie pracowników i studentów tego ośrodka naukowego, prace zbiorowe oraz artykuły dowiódł, iż uczeni ci kategorycznie sprzeciwiali się rządowemu laickiemu projektowi reformy osobowego prawa małżeńskiego, broniąc katolickiej nauki o małżeństwie. Znalazło to w sposób szczególny wyraz w dwóch pozycjach, jakimi były prace zbiorowe: „Małżeństwo w świetle nauki katolickiej” oraz „Rozbiór krytyczny projektu prawa małżeńskiego uchwalonego przez K. K”.
Ustosunkowując się do poziomu merytorycznego publikacji Autor wykazał, iż są one oparte na solidnym materiale źródłowych i literaturze przedmiotu. W opinii Autora, na szczególną uwagę zasługują: doktorat Piotra Bobera „Pojęcie tajnej przeszkody małżeńskiej w prawie kanonicznym” oraz praca licencjacka Teodora Benscha „Wpływ chorób umysłowych na ważność umowy małżeńskiej w prawie kanonicznem”. W obu bowiem pozycjach kanoniści podjęli tematykę w owych czasach na wskroś nowatorską wieńcząc wyniki swych badań rzetelnymi podsumowaniami.
10.2174/0250688202666210402094638
Authors: Mahadevappa Manjappa , Kulkarni Prashanth , Poornima KS , Prasad Rajendra 
Published: April 2021

Background:
Lactic acidosis of any etiology is associated with poor prognosis and often fatal. The survival in lactic acidosis depends on the ability to identify
and remove the cause and managing organ perfusion. Nucleoside Reverse Transcriptase Inhibitors (NRTIs), which are used widely in the treatment
of Human Immune Deficiency Virus (HIV) infection, are known to cause life-threatening Type B lactic acidosis.

Case Presentation:
Severe lactic acidosis can cause organ failure, especially in respiratory and cardiovascular systems. However, the role of acute lactic acidosis in
these organ failures is mostly suggested by experimental studies in animals or in vitro, as few clinical studies in humans are available. Acidosis
elevates pulmonary vascular resistance, giving rise to pulmonary hypertension and peripheral vasodilation with an associated fall in blood pressure
and systemic hypotension. Although lactic acidosis presenting as abdominal pain, vomiting, and pancreatitis have been reported in persons
receiving antiretroviral therapy, the presentation as acute right heart failure has rarely been documented in the literature. We report a case of severe
metabolic and lactic acidosis in a patient receiving stavudine and lamivudine, with a rare and atypical presentation as acute right heart failure and
severe pulmonary hypertension.

Conclusion:
This case emphasizes the need to consider lactic acidosis as a potential cause in patients presenting with acute unexplained right heart failure and
severe pulmonary hypertension.
FEN1 Status and Its Correlation with Clinicopathologic Characteristic in
Colorectal Cancer
Authors: Guo Yundi , Du Zixuan , Zhou Yuanshuai , Sun Haijun , Liang Rui , Sun Min-Xuan , Tang Zaixiang , Liu Song-Bai 
Published: April 2021

Objective::
The goal of this study was to investigate the status of FEN1 in colorectal
cancer (CRC) and determine the potential correlation between FEN1 expression level and
clinicopathological parameters in CRC patients.

Methods::
Expression of FEN1 in CRC tissue on tissue microarray was detected using
immunohistochemistry (IHC). The relationship between FEN1 expression status and
clinicopathologic characteristics of CRC was analyzed by the Chi-square test. The survival data of
TCGA Colon Cancer (COAD) were obtained from ucsc xena browser (https://xenabrowser.net/).
Patients were separated into higher and lower expression groups by median FEN1 expression. The
association with prognosis of CRC patients was determined by Kaplan-Meier survival analysis
with Log-rank test.

Results::
FEN1expression level and cellular localization had wide variability among different
individuals; we classified the staining results into four types: both positive in nucleus and
cytoplasm, both negative in nucleus and cytoplasm, only positive in the nucleus, only positive in
the cytoplasm. Moreover, FEN1 expression status only correlated with patient’s metastasis status,
and the patients in the NLCL group showed more risk of cancer cell metastasis.

Conclusion::
Our results indicate that FEN1 expression level and cellular localization had wide
variability in CRC and is not a promising biomarker in CRC.
A Brief Review of circRNA Biogenesis, Detection, and Function
Authors: Liang Ying , Liu Niannian , Yang Le , Tang Jianjun , Wang Yinglong , Mei Meng 
Published: April 2021

:
Circular RNA (circRNA) is a non-coding molecule produced through alternative splicing
of one or more exons of a gene in the presence of an RNA-induced silencing complex (RISC).
Its formation depends on complementary intron sequences on both sides of the circularized sequence.
CircRNA functions as a sponge for miRNA, playing the role of the transcriptional regulator
or potential biomarker. It has an impact on fetal growth and on synaptic facilitation in the brain.
In this review, we illustrate biogenesis mechanisms, characteristics, and functions of cirRNAs. We
also summarize methods using sequence feature and RNA next-generation sequencing data for circRNA
prediction. Finally, we discuss the state of the research on circRNA in diseases, which will
bring new contributions to future disease treatments.