bluuebunny/crossref_metadata_2025_split huggingface dataset:
RAMS11 promotes CRC through mTOR-dependent inhibition of autophagy, suppression of apoptosis, and promotion of epithelial-mesenchymal transition
Authors: KHAN Md Zahirul ISLAM , LAW Helen Ka-Wai 
Published: March 2021

Abstract
Background

Long non-coding RNAs (lncRNAs), a class of non-coding RNAs (ncRNAs) associated with diverse biological processes of cells. Over the past decades, cumulating research evidences revealed that abnormal expressions of lncRNAs are associated with colorectal cancer (CRC) initiation, progression, metastasis, and resistance to therapies. Moreover, their usefulness as candidate biomarkers for CRC diagnosis and prognosis are well evident throughout previous literature. In the current study, we examined the role and molecular mechanisms of newly identified lncRNA named RNA associated with metastasis-11 (RAMS11) in CRC development.

Methods

The expression of RAMS11 in CRC cell lines DLD-1, HT-29, HCT-116, and SW480 and colon normal cells CCD-112-CoN were evaluated by quantitative RT-qPCR. The results showed that the RAMS11 is significantly upregulated in CRC cell lines compared to the normal cells. The CCK-8 proliferation assay, colony formation assay, and migration assay were performed to evaluate the biological and physiological functions of RAMS11 in vitro . To decipher the molecular mechanisms of RAMS11 medicated CRC progression, we further performed western blot analysis of the key pathway proteins (e.g., AMPK, AKT, and mTOR).

Results

Our results revealed that higher expression of RAMS11 is associated with increased CRC proliferation, migration, and development of metastasis. Knockdown of RAMS11 induced autophagy, apoptosis along with reduction of epithelial-mesenchymal transition (EMT) suggesting that RAMS11 is involved in CRC progression. The molecular mechanisms of RAMS11 indicated that knockdown of RAMS11 significantly inhibited CRC carcinogenesis through mTOR-dependent autophagy induction.

Conclusions

In sum, our results suggested that RAMS11 is an important oncogene in CRC pathogenesis. Targeting RAMS11 could be a potential therapeutic strategy for CRC management.
Source and pattern identification of ground deformation based on the framework of Blind Source Separation--Nonnegative Matrix Factorization: a case study in a long-term GPS monitoring mine
Authors: Gu hongyu , Ma fengshan , Lin Liangjun , wang donghui , Tie yongbo , Chen weichang 
Published: March 2021

Abstract
Ground deformation caused by mining affects the safety of underground mining and buildings. High-resolution characterization of ground deformation respond to latent sources is a first step toward improved hazard forecasting. We rely on long-term GPS displacement data and an improved NMF algorithm of BSS to identify the sources driving ground deformation. The NMF identifies three sources (S1, S2, and S3) with distinct spatial and temporal surface deformation patterns and quantitatively reveals the contribution of each source to ground deformation. S1 captures horizontal Y-displacement related to horizontal tectonic stress \({{\sigma }}_{1}\). S2 dominates vertical Z-displacement with a widespread isotropous deformation driven by self-weight body force. S3 controls horizontal X-displacement related to horizontal tectonic stress \({{\sigma }}_{2}\). Besides, we find that independent contributions of these three sources can be resolved from the GPS data. The results show that the sharp change of source contribution and even transformation of dominate source are highly related to the severe deformation belt. This phenomenon is time independent, and helps us to select sites and find the potential risk area at its early stage.
Pig genome functional annotation enhances biological interpretations of complex traits and comparative epigenomics
Authors: Zhou Huaijun , Pan Zhangyuan , Yao Yuelin , Ying Hongwei , Cai Zexi , Wang Ying , Bai Lijing , Kern Colin , Halstead Michelle , Chanthavixay Kelly , Trakooljul Nares , Wimmers Klaus , Sahana Goutam , Su Guosheng , Lund Mogens Sandø , Fredholm Merete , Karlskov-Mortensen Peter , Ernst Catherine , Ross Pablo , Tuggle Christopher , Fang Lingzhao 
Published: March 2021

Abstract
The functional annotation of livestock genomes is crucial for understanding the molecular mechanisms that underpin complex traits of economic importance, adaptive evolution and comparative genomics. Here, we provide the most comprehensive catalogue to date of regulatory elements in the pig (Sus scrofa) by integrating 223 epigenomic and transcriptomic data sets, representing 14 biologically important tissues. We systematically describe the dynamic epigenetic landscape across tissues by functionally annotating 15 different chromatin states and defining their tissue-specific regulatory activities. We demonstrate that genomic variants associated with complex traits and adaptive evolution in pig are significantly enriched in active promoters and enhancers. Furthermore, we reveal distinct tissue-specific regulatory selection between Asian and European pig domestication processes. Compared with human and mouse epigenomes, we show that porcine regulatory elements are more conserved in DNA sequence, under both rapid and slow evolution, than those under neutral evolution across pig, mouse, and human. Finally, we provide novel biological insights on tissue-specific regulatory conservation and demonstrate that, depending on the traits, mouse or pig might be more appropriate biomedical models for different complex traits and diseases in humans through integrating comparative epigenomes with 47 human genome-wide association studies.
Differences between genetic dilated cardiomyopathy and myocarditis in children presenting with severe cardiac dysfunction.
Authors: Gran Ferran , Fidalgo Andrea , Dolader Paola , Garrido Marta , Navarro Alexandra , Izquierdo-Blasco Jaume , Balcells Joan , Codina-Sola Marta , Fernandez-Alvarez Paula , Sabaté-Rotés Anna , Betrián Pedro , Fernández-Doblas Joaquín , Abella Raúl , Roses-Noguer Ferran 
Published: March 2021

Abstract
Background Acute myocarditis is an inflammatory disease of the myocardium, and it can present as severe heart failure in children. Differential diagnosis with genetic cardiomyopathy can be difficult. The objective of this study is to identify patterns of clinical presentation and to assess invasive and non-invasive measures to differentiate patients with acute myocarditis from patients with dilated genetic cardiomyopathy. Material and Methods We performed a retrospective descriptive study of all paediatric patients (0-16 years old) that presented with new-onset heart failure with left ventricle ejection fraction <35% in whom we performed an endomyocardial biopsy (EMB) during the period from April 2007 to December 2020. Results The patients were classified into two groups: Group 1 included 17 patients with myocarditis. Group 2 included 9 patients with genetic cardiomyopathy. Findings favouring a diagnosis of myocarditis included a fulminant or acute presentation (77.8% vs 33.3%, p=0.01), higher degree of cardiac enzyme elevation (p=0.011), lower left ventricular dimension z-score (2.2 vs. 5.4, p = 0.03) increase of ventricular wall thickness (88.8% vs 33.3%, p=0.03), and oedema in the endomyocardial biopsy. Seven (77.8%) patients with genetic cardiomyopathy had inflammation in the endomyocardial biopsy, fulfilling the diagnostic criteria of inflammatory cardiomyopathy. Conclusions Differentiate patients with a myocarditis from those with genetic cardiomyopathy can be challenging, even performing an EMB. Some patients with genetic cardiomyopathy fulfil the diagnostic criteria of inflammatory cardiomyopathy. Using invasive and non-invasive measures may be useful to develop a predictive model to differentiate myocarditis from genetic cardiomyopathy.
Genetic Polymorphism and Intravenous Immunoglobulin Resistance Relationship in Kawasaki Disease
Authors: Varlı Yusuf Ziya , Caglayan Ahmet Okay , Bilguvar Kaya , Gunel Murat , Oztarhan Kazim 
Published: March 2021

Abstract
Objective
Kawasaki disease (KD) is an acute febrile systemic vasculitis and the most common cause of coronary artery aneurysm (CAA) in children. Intravenous immunoglobulin (IVIG) therapy is used to prevent fever and systemic inflammation. However, IVIG resistance is the most important risk factor of morbidity and mortality. It has been identified several single nucleotide polymorphisms (SNPs) related to IVIG resistance and this research aims to analyze these polymorphisms in our study population.
Methods
Patients diagnosed with KD (n:259) were analyzed retrospectively. Blood samples were taken from a randomized subgroup (n:97). Previously reported IVIG resistance related exonic SNPs at five different gene loci (IL16, TNFSF14, NFATC2, DERL3, SAMD9L) were evaluated by whole exome sequencing (WES).
Results
Between 2010–2019, 259 patients (male/female: 1,67) with KD were submitted to our clinic. CAA and IVIG resistance rates were 11.6% and 21.6%, respectively. The risk of developing CAA was significantly increased in patients with IVIG resistance (p < 0.001). As a result, IVIG resistance frequency increased in the presence of three SNPs. These are "rs11556218"(p.Asn1147Lys), "rs344560"(p.Lys214Glu), "rs12479626"(p.His446Arg), and are located in IL16, TNFSF14, NFATC2 genes, respectively.
Conclusions
Until now, KD-related genetic data mostly obtained from studies involving large cohorts from Northeast Asian countries. In the analysis of this largest Turkish cohort in the literature, we found that, similar with previous studies, the IL16 gene may be plays important role in the IVIG resistance mechanism.
The Relationship Between Vitamin D Serum Levels and Rheumatoid Arthritis Activity in a Sample of Syrian Patients- a cross-sectional observational study
Authors: Alourfi Zaynab , Aldahhan Nisreen 
Published: March 2021

Abstract
Background. Rheumatoid Arthritis (RA) is a systemic auto immune disease that mainly affects joints. It might cause functional disability and increased morbidity and mortality.Vitamin D was reported to have an immunomodulation role in reducing the inflammation process in many auto immune diseases; RA is one of them.The aim was to determine the relationship between serum vitamin D levels and rheumatoid arthritis activity in a sample of Syrian patients.Methods This cross-sectional observational study was carried out in Al-Mouwasat and Al-Assad University Hospitals(Damascus University - Syria). RA patients were diagnosed according to the American College of Rheumatology (ACR) and the European League Against Rheumatism(EULAR)classification criteria for rheumatoid arthritis 2010 standards.Results Vitamin D was significantly higher in RA patients in remission (27.92 ± 13 ng/ml) than in patients with active RA (21.22 ± 13 ng/ml). There was an inverse relationship between serum vitamin D levels and RA activity.Conclusion Serum vitamin D levels were inversely correlated with RA activity in a sample of Syrian RA patients.
Essential List of Medicinal Products for Rare Diseases – Recommendations from the IRDiRC Rare Disease Treatment Access Working Group
Authors: Gahl William A. , Wong-Rieger Durhane , Hivert Virginie , Yang Rachel , Zanello Galliano , Groft Stephen 
Published: March 2021

Abstract
Background
Treatments are often unavailable for rare disease patients, especially in low-and-middle-income countries. Reasons for this include lack of financial support for therapies and onerous regulatory requirements for approval of drugs. Other barriers include lack of reimbursement, administrative infrastructure, and knowledge about diagnosis and drug treatment options. The International Rare Diseases Research Consortium set up the Rare Disease Treatment Access Working Group with the first objective to develop an essential list of medicinal products for rare diseases.
Results
The Working Group extracted 215 drugs with Orphan designation in the FDA, EMA databases and/or China’s Rare Diseases Catalog. The drugs were organized in seven disease categories: metabolic, neurologic, hematologic, anti-inflammatory, endocrine, pulmonary, and immunologic, plus a miscellaneous category.
Conclusions
The proposed list of essential medicinal products for rare diseases is intended to initiate discussion and collaboration among patient advocacy groups, health care providers, industry and government agencies to enhance access to appropriate medicines for all rare disease patients throughout the world.
Importance of daptomycin dosage on the clinical outcomes in early post-liver transplant recipients with vancomycin-resistant enterococci infection
Authors: Lee Ing-Kit , Sng Yi-Ping , Li Wei-Feng , Chen Chao-Long , Wang Chih-Chi , Lin Chih-Che , Chen I-Ling 
Published: March 2021

Abstract
Background: The prevalence of vancomycin-resistant enterococci (VRE) is increasing among liver transplant recipients. This study aimed to explore the clinical features of liver transplant recipients with VRE infection/colonization and to determine the impact of daptomycin dosage on the outcomes. Methods: We retrospectively enrolled pre-transplant and post-transplant patients with VRE colonization/infection from 2016 to 2019. Results: Altogether, 428 patients underwent liver transplantation. Among these, 22 (5.1%) patients developed VRE colonization/infection. All VRE isolates were Enterococcus faecium. Two (9%) patients acquired VRE in the pre-transplant period, 16 (3 colonizations and 13 infections) (72.7%) in the early post-liver transplant period (≤60-day after transplantation), and 4 (2 colonization and 2 infections) (18.1%) in the late post-liver transplant period (>6-month after transplantation). Among 13 patients with early post-liver transplant VRE infection, 12 (92.3%) underwent living-donor liver transplantation and 1 underwent deceased donor liver transplantation. Among these 13 patients, the median time from transplant to emergence of VRE infection was 12 days. The median interval from VRE infection to death was 27 days and the 30-day mortality was 67%. Of these 13 patients, eleven patients (8 survived; 3 died) received daptomycin therapy for VRE. Among them, 4 (36.3%) received daptomycin doses <8 mg/kg. Non-survivors (n=3) received significantly lower daptomycin dose than survivors (n=8) (P=0.040). Daptomycin doses <8mg/kg were more frequently associated with non-survivors (n=3) than with survivors (n=8) (P=0.024). Conclusions: In summary, the suboptimal dosage of daptomycin may have contributed to a higher rate of in-hospital mortality. Doses ≥8 mg/kg may be needed to adequately treat VRE infection in early post-liver transplant recipients.Level of evidence: Level III
Association of Short-term Particulate Matter Exposure with Suicide Death Among Major Depressive Disorder Patients: A Time-Stratified Case-Crossover Analysis
Authors: Hwang In Young , Choi Daein , Kim Jihoon Andrew , Choi Seulggie , Chang Jooyoung , Goo Ae Jin , Ko Ahryoung , Lee Gyeongsil , Kim Kyae Hyung , Son Joung Sik , Park Sang Min 
Published: March 2021

Abstract
Objective: There is growing evidence that suggests a potential association between particulate matter (PM) and suicide. However, it is unclear that PM exposure and suicide death among major depressive disorder (MDD) patients, a high-risk group for suicide.Methods: We investigated the risk of suicide among 1,046,169 newly-diagnosed MDD patients from 2004 to 2015 within the Korean National Health Insurance Service (NHIS) database. We identified 3,372 suicide cases from January 1, 2015, to December 31, 2017, within the death statistics database of the Korean National Statistical Office. PMs with diameter less than 2.5 μm (PM2.5), less than 10 μm (PM10), and 2.5 μm to 10 μm (PM2.5-10) were considered, which were provided from the National Ambient Air Monitoring System in South Korea. Time-stratified case-crossover analysis was performed to investigate the association of particulate matter exposure to suicide events.Results: The risk of suicide was significantly high upon the high level of exposure to PM2.5-10, PM10 on lag 1 (p for trend = 0.044, 0.035, respectively). A similar association was observed in the multi-day lag model (lag 0-3). Increasing exposure to PM 2.5 was not associated with increased suicide risk.Conclusions: Short-term exposure to a high level of PM2.5-10 and PM10 was associated with an elevated risk for suicide among MDD patients, while PM2.5 did not. There is a clear dose-response relationship between short-term coarse particle exposures with suicide death among Major Depressive Disorder patients. This result will be used as an essential basis for consideration when establishing an air pollution alarm system and implementing a suicide prevention program for reducing adverse health outcomes by PM.
Radiotherapy efficacies for vertebral hemangioma patients with severe spinal cord compression and cauda equina syndrome
Authors: Chen Yi-Lun , Yang Shih-Huang , Kuo Sung-Hsin 
Published: March 2021

Abstract
Background: Although vertebral hemangioma is the most common benign spine tumor, rare patients may experience spinal cord compression accompanied by pain and neurological deficits, in whom surgery remains the standard treatment option. For patients with comorbidities who cannot tolerate operation, radiotherapy may be an alternative treatment, however, previous studies suggested that if spinal cord cannel was aggressively compressed by vertebral hemangioma, the efficacies of radiotherapy were suboptimal. Methods and results: Herein, we presented two patients with vertebral hemangioma who developed spinal cord compression, first patient with underlying congenital heart disease had spinal canal encroachment of 63.4% at ninth thoracic vertebra, and second patient failed to transcatheter arterial embolization for treating total encroachment of sacrum plexus. The first patient underwent radiotherapy with 44 Gy at 20 fractions using Tomotherapy and became fully recovered from the severe neurological deficits at 5.5 months after completing radiotherapy. The second patient benefited from neurological symptoms relief at 6 months after completion of radiotherapy using 42 Gy at 21 fractions using volumetric modulated arc therapy (VMAT) technique.Conclusions: Our results indicate that radiotherapy with 42 to 44 Gy using conventional fractions could efficiently cause tumor regressions and improve neurological symptoms resulted from vertebral hemangioma-causing spinal cord compression.
Microplastics induced endocrine disruption, alteration in testicular tissue in tilapia (Oreochromis niloticus) pre-fed on Amphora coffeaeformis
Authors: Ismail Rania F. , Saleh Norhan E. , Sayed Alaa El-Din H. 
Published: March 2021

Abstract
This study examines the potential defending effects of the diatom, Amphora coffeaeformis, as a feed additive against the deleterious effects (mainly on gonads) caused by microplastics (MPs) in the Nile tilapia, Oreochromes niloticus. Male tilapia groups were pre-fed diets with four different supplementation levels of A. coffeaeformis (0%, 2.5%, 5% and 7.5%) for 70 days, then were exposed to 10 mg/L MPs for 15 days. The results showed significantly (p < 0.05) higher numbers of erythrocytes, but significant decreases (p < 0.05) in the number of RBCs, Hb, Ht, platelets, and eosinophil percentages. Testicular histological degenerative changes and testis-ova were found in the MPs-exposed fish. Thus, A. coffeaeformis supplementation displayed ameliorative properties that detoxified the negative effects of MPs. This study provides a better understanding of the reproductive injuries caused by MPs and provides evidence for the use of A. coffeaeformis as a natural remedy in freshwater tilapia.
Association of SYNTAX score with myocardial injury in STEMI patients: a cardiac magnetic resonance study
Authors: Gao Guangren , Feng Lianrong , Fu Jinguo , Huo Zhaoyang , Zhang Lei , Zheng Ruishuang , Wang Lei , Liu Jing , Niu Heping , Liu Fenghai , Kang Liqing , Liu Jieqiong , Liu Hui , Zhang Xinru , Zhang Jun 
Published: March 2021

Abstract
SYNTAX score (SS) is positively correlated with postprocedural myocardial injury identified by high sensitivity cardiac troponin in patients undergone elective coronary artery intervention, evidences about the association of SS with myocardial injury in STEMI patients were still scarce. A total of 149 consecutive patients within 24 h of STEMI were enrolled in the study. Both angiography and cardiac magnetic resonance (CMR) were performed during hospitalization. The time was 7.05 h (4.44,95.91, IQR) from symptom to angiography and 7.31 ± 2.60 days from symptom to CMR. The total median SS was 17(9–25, IQR). In terms of myocardial injury parameters, there was a positive correlation between SS and infarct size (IS) (p < 0.001, Spearman r = 0.292), and negative correlation between SS and myocardial salvage index (MSI) (p < 0.001, Spearman r=-0.314). There was no significant correlation between SS and area at risk, microvascular obstruction or intramyocardial hemorrhage. According to SS, patients were divided into low SS (< 22) (LSS)(n = 96) or mediate-high SS (≥ 22) (MHSS)(n = 53) group. In the multivariable model, after adjustment for important known predictors of IS, MHSS was significantly associated with high IS (≥ mean 35.43) (odd ratio = 2.245, 95% confidence interval [1.002–5.053], p = 0.048), as a continuous variate, SS was also significant associated with high IS (odd ratio = 1.053, 95% confidence interval [1.014–1.095], p = 0.008). The areas under the receiver operating characteristic curves of SS for high IS and low MSI were 0.664 and 0.610. Conclusion: of STEMI patients who presented to hospital within 24h from symptom onset, SS was positively related with IS and negatively with MSI. SS was an independent predictor of IS after adjusting for important covariates.
Searching for refractory plasmonic materials: the structural and optical properties of Au3Zr intermetallic thin films
Authors: Littlehailes Hugh , Hendren William R , Drakeley Stacey , Bowman Robert M , Huang Fumin 
Published: March 2021

Abstract
Optical properties of refractory intermetallic thin films of Au 3 Zr were experimentally investigated for the first time, which show distinctive plasmonic properties in the visible and near infrared region. The films were fabricated through DC magnetron sputtering at various deposition temperature ranging from room temperature to 427°C and annealed at different vacuum levels. Both the structural and optical properties are found to be critically dependent on deposition temperature and anneal conditions. Films deposited between 205-320°C are shown to exhibit lower negative permittivity and better thermal stability, which could be linked to specific crystalline orientations. The films are stable when annealed at 10 -8 Torr, but are partially oxidized when annealed at 10 -6 Torr, suggesting oxidization could be a restricting issue for high-temperature applications in ambient environment.