bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
Authors: Heiden Stefanie , Siwek Rebecca , Lotz Marie-Luise , Borkowsky Sarah , Schröter Rita , Nedvetsky Pavel , Rohlmann Astrid , Missler Markus , Krahn Michael P. 
Published: March 2021

AbstractApical-basal polarity is a key feature of most epithelial cells and it is regulated by highly conserved protein complexes. In mammalian podocytes, which emerge from columnar epithelial cells, this polarity is preserved and the tight junctions are converted to the slit diaphragms, establishing the filtration barrier. In Drosophila, nephrocytes show several structural and functional similarities with mammalian podocytes and proximal tubular cells. However, in contrast to podocytes, little is known about the role of apical-basal polarity regulators in these cells. In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and slit diaphragm assembly/maintenance of nephrocytes is regulated by classical apical-basal polarity regulators, which have distinct functions in endocytosis.
Efficiency of the skeletonized Pendulum K appliance for non-compliance maxillary molar distalization
Authors: Kinzinger Gero Stefan Michael , Hourfar Jan , Lisson Jörg Alexander 
Published: March 2021

Abstract
Purpose
Conventional anchorage with exclusively intraorally anchored appliances for non-compliance molar distalization combines a palatal acrylic button with periodontal anchorage. This type of anchorage is critically discussed because of the temporary hygienic impairment of the palate and the uncertain anchoring quality of the button. A purely dentally/periodontally anchored Pendulum K appliance was developed, which is exclusively anchored via four occlusal rests. The aims of this pilot study were to examine the suitability of the skeletonized Pendulum K for distalization of maxillary molars, and to investigate the quality of this alternative anchoring modality.

Patients and methods
In all, 10 patients received skeletonized Pendulum K appliances attached to all maxillary premolars for bilateral molar distalization. Supporting anchorage through an acrylic button adjacent to the anterior palate was not used. The pendulum springs were initially activated on both sides with a distalization force of 220 cN each and provided with uprighting and toe-in bends. The specific force/moment system was regularly reactivated intraorally by adjustment of the distal screw.

Results
The study demonstrates the suitability of the skeletonized Pendulum K appliance for the distalization of maxillary molars (3.28 ± 0.73 mm). Side effects on the molars were slight distal tipping (3.50 ± 2.51°/PP, 3.00 ± 1.41°/SN) and mesial inward rotation (average 2.75 ± 7.50° and 4.50 ± 12.77°). Significant anchorage loss occurred in the form of mesialization of the incisors by 1.40 ± 0.82 mm and of the first premolars by 2.28 ± 0.85 mm.

Conclusion
The skeletonized Pendulum K appliance allows compliance-free upper molar distalization. Exclusively dental/periodontal anchorage resulted in a lower percentage of molar distalization compared to a conventional anchoring preparation of the Pendulum K with a palatal acrylic button. Anchorage loss had a comparatively stronger effect on the anchoring premolars but less on the incisors. Typical side effects on the molars such as distal tipping and mesial inward rotation were remarkably low.
Magnetic resonance metrics to evaluate the effect of therapy in amyotrophic lateral sclerosis: the experience with edaravone
Authors: Distaso Eugenio , Milella Giammarco , Mezzapesa Domenico Maria , Introna Alessandro , D’Errico Eustachio , Fraddosio Angela , Zoccolella Stefano , Dicuonzo Franca , Simone Isabella Laura 
Published: March 2021

Abstract
Background
Edaravone was approved as a new treatment for amyotrophic lateral sclerosis (ALS), although there are different opinions on its effectiveness. Magnetic resonance (MRI) measures appear promising as diagnostic and prognostic indicators of disease. However, published studies on MRI using to monitor treatment efficacy in ALS are lacking.

Purpose
The objective of this study was to investigate changes in brain MRI measures in patients treated with edaravone.

Methods
Thirteen ALS patients assuming edaravone (ALS-EDA) underwent MRI at baseline (T0) and after 6 months (T6) to measure cortical thickness (CT) and fractional anisotropy (FA) of white matter (WM) tracts. MRI data of ALS-EDA were compared at T0 with those of 12 control subjects (CS), and at T6 with those of 11 ALS patients assuming only riluzole (ALS-RIL), extracted from our ALS cohort using a propensity-score-matching. A longitudinal MRI analysis was performed in ALS-EDA between T6 and T0.

Results
At T0, ALS-EDA showed a cortical widespread thinning in both hemispheres, particularly in the bilateral precentral gyrus, and a reduction of FA in bilateral corticospinal tracts, in comparison to CS. Thinning in bilateral precentral cortex and significant widespread reduction of FA in several WM tracts were observed in ALS-EDA at T6 compared to T0. At T6, no significant differences in MRI measures of ALS-EDA versus ALS-RIL were found.

Conclusions
Patients treated with edaravone showed progression of damage in the motor cortex and several WM tracts, at a six-month follow-up. Moreover, this study showed no evidence of a difference between edaravone and riluzole.
Sodium–glucose cotransporter 2 inhibitors as an add-on therapy to insulin for type 1 diabetes mellitus: Meta-analysis of randomized controlled trials
Authors: Rao Lunwen , Ren Chenhong , Luo Shan , Huang Chenghu , Li Xuefeng 
Published: March 2021

Abstract
Aims
The aim was to systematically review the efficacy and safety of sodium–glucose cotransporter inhibitor (SGLT2i) as an adjunct to insulin at different follow-up durations in randomized, double-blind clinical trials in patients with type 1 diabetes.

Methods
We conducted a search on Medline, Embase, and the Cochrane Library for relevant studies published before May 2020. According to the duration of follow-up, the subgroup analysis included four periods: 1–4, 12–18, 24–26, and 52 weeks. In the five trials included both 24–26 and 52 weeks of follow-up, we compared the efficacy by the placebo-subtracted difference and changes in SGLT2i groups.

Results
Fifteen trials including 7109 participants were analyzed. The combination of SGLT2i and insulin improved hemoglobin A1c (HbA1c), fasting plasma glucose (FPG), daily insulin dose, body weight, and blood pressure, which varied greatly by different follow-ups. Compared with %HbA1c at 24–26 weeks, placebo-subtracted differences and changes in the SGLT2i groups slightly increased. SGLT2i plus insulin treatment showed no difference in the occurrence of urinary tract infections (UTIs), hypoglycemia, or severe hypoglycemia but increased the risk of genital tract infections (GTIs) in a duration-dependent manner. SGLT2i treatment was associated with a significantly higher rate of ketone-related SAEs and diabetic ketoacidosis (DKA) at 52 weeks.

Conclusion
SGLT2i as an add-on therapy to insulin improved glycemic control and body weight and decreased the required dose of insulin without increasing the risk of hypoglycemia. However, after 6 months the benefits of SGLT2is on glycemic control may weaken and the risks of GTIs and DKA increased.
Non-credible symptom report in the clinical evaluation of adult ADHD: development and initial validation of a new validity index embedded in the Conners’ adult ADHD rating scales
Authors: Becke Miriam , Tucha Lara , Weisbrod Matthias , Aschenbrenner Steffen , Tucha Oliver , Fuermaier Anselm B. M. 
Published: March 2021

AbstractAs attention-deficit/hyperactivity disorder (ADHD) is a feasible target for individuals aiming to procure stimulant medication or accommodations, there is a high clinical need for accurate assessment of adult ADHD. Proven falsifiability of commonly used diagnostic instruments is therefore of concern. The present study aimed to develop a new, ADHD-specific infrequency index to aid the detection of non-credible self-report. Disorder-specific adaptations of four detection strategies were embedded into the Conners’ Adult ADHD Rating Scales (CAARS) and tested for infrequency among credible neurotypical controls (n = 1001) and credible adults with ADHD (n = 100). The new index’ ability to detect instructed simulators (n = 242) and non-credible adults with ADHD (n = 22) was subsequently examined using ROC analyses. Applying a conservative cut-off score, the new index identified 30% of participants instructed to simulate ADHD while retaining a specificity of 98%. Items assessing supposed symptoms of ADHD proved most useful in distinguishing genuine patients with ADHD from simulators, whereas inquiries into unusual symptom combinations produced a small effect. The CAARS Infrequency Index (CII) outperformed the new infrequency index in terms of sensitivity (46%), but not overall classification accuracy as determined in ROC analyses. Neither the new infrequency index nor the CII detected non-credible adults diagnosed with ADHD with adequate accuracy. In contrast, both infrequency indices showed high classification accuracy when used to detect symptom over-report. Findings support the new indices’ utility as an adjunct measure in uncovering feigned ADHD, while underscoring the need to differentiate general over-reporting from specific forms of feigning.
Novel photosensitizer for dye-sensitized solar cell based on ionic liquid–doped blend polymer electrolyte
Authors: Devadiga Dheeraj , Selvakumar M. , Shetty Prakasha , Mahesha M. G. , Devadiga Deepak , Ahipa T. N. , Kumar S. Senthil 
Published: March 2021

AbstractThe existing energy situation demands not only the huge energy in a short time but also clean energy. In this regard, an integrated photo-supercapacitor device has been fabricated in which photoelectric conversion and energy storage are achieved simultaneously. A novel carbazole-based dye is synthesized and characterized for photosensitizer. The silver-doped titanium dioxide (Ag-TiO2) is synthesized, and it is used as photoanode material. Different concentrations of tetrabutylammonium iodide (TBAI)-doped polyvinyl alcohol–polyvinylpyrrolidone (PVA-PVP) blend polymer electrolytes are prepared, and their conductivity and dielectric properties were studied. Reduced graphene oxide (r-GO) is synthesized by a one-pot synthesis method and confirmed using Raman spectroscopy for counter electrode material in dye-sensitized solar cell (DSSC) and supercapacitor electrodes. The DSSC having 4% Ag-TiO2–based photoanode showed the highest efficiency of 1.06% (among r-GO counter electrodes) and 2.37% (among platinum counter electrodes). The supercapacitor before integration and after integration exhibits specific capacitance of 1.72 Fg−1 and 1.327 Fg−1, respectively.
Into a Fluoroless Future: an Appraisal of Fluoroscopy-Free Techniques in Clinical Cardiac Electrophysiology
Authors: Purtell Christopher S. , Kipp Ryan T. , Eckhardt Lee L. 
Published: March 2021

Abstract
Purpose of Review
There are risks to both patients and electrophysiology providers from radiation exposure from fluoroscopic imaging, and there is increased interest in fluoroscopic reduction. We review the imaging tools, their applications, and current uses to eliminate fluoroscopy.

Recent Findings
Multiple recent studies provide supporting evidence for the transition to fluoroscopy-free techniques for both ablations and device implantation. The most frequently used alternative imaging approaches include intracardiac echocardiography, cardiac MRI guidance, and 3D electroanatomic mapping systems. Electroanatomic mapping and intracardiac echocardiography originally used to augment fluoroscopy imaging are now replacing the older imaging technique. The data supports that the future of electrophysiology can be fluoroscopy-free or very low fluoroscopy for the vast majority of cases.

Summary
As provider and institution experience grows with these techniques, many EP labs may choose to completely forego the use of fluoroscopy. Trainees will benefit from early experience with these techniques.
KRIT1 Gene in Patients with Cerebral Cavernous Malformations: Clinical Features and Molecular Characterization of Novel Variants
Authors: Ricci Claudia , Cerase Alfonso , Riolo Giulia , Manasse Giuditta , Battistini Stefania 
Published: March 2021

AbstractCerebral cavernous malformations (CCMs) are vascular malformations that may result in headaches, seizures, focal neurological deficits, and hemorrhage. CCMs occur sporadically (80%) or in familial form (20%), with autosomal dominant inheritance. Among the three CCM-related genes, mutations in KRIT1 account for 53–65% of familial cases and more than 100 different mutations have been identified so far. In the present work, we describe the clinical, neuroradiological, and genetic findings of sixteen CCM Italian patients, 13 belonging to 4 unrelated families and 3 sporadic cases. Six distinct KRIT1 gene variants, two novel (c.1730+1_1730+3del, c.1664 C>T) and four previously described (c.966G>A, c.1255-1G>A c.1197_1200del, c.1255-1_1256del), were identified, including a possible de novo mutation. All the variants resulted in a premature stop codon. Cerebral 1.5 T magnetic resonance imaging showed multiple CCMs in all the mutation carriers for whom it was available, including sporadic cases. One patient had also cutaneous angiomas. Among the mutation carriers, symptomatic patients constituted 66% and a variable phenotypic expression was observed. Our data confirms phenotypic variability and incomplete penetrance of neurological symptoms in KRIT1-positive families, expands the mutational spectrum of this gene, and highlights how sporadic cases with multiple lesions need an approach similar to individuals with familial CCM.
Forecasting emergency medicine reserve demand with a novel decomposition-ensemble methodology
Authors: Jiang-ning Li , Xian-liang Shi , An-qiang Huang , Ze-fang He , Yu-xuan Kang , Dong Li 
Published: March 2021

AbstractAccurate prediction is a fundamental and leading work of the emergency medicine reserve management. Given that the emergency medicine reserve demand is affected by various factors during the public health events and thus the observed data are composed of different but hard-to-distinguish components, the traditional demand forecasting method is not competent for this case. To bridge this gap, this paper proposes the EMD-ELMAN-ARIMA (ELA) model which first utilizes Empirical Mode Decomposition (EMD) to decompose the original series into various components. The Elman neural network and ARIMA models are employed to forecast the identified components and the final forecast values are generated by integrating the individual component predictions. For the purpose of validation, an empirical study is carried out based on the influenza data of Beijing from 2014 to 2018. The results clearly show the superiority of the proposed ELA algorithm over its two rivals including the ARIMA and ELMAN models.
Influence of direct electric field on PMCG-alginate-based microcapsule
Authors: Kasak Peter , Sasová Jana , Shoheeduzzaman Ruqaia , Baig Mirza T. , Alyafei Aldana Ali H. A. , Tkac Jan 
Published: March 2021

AbstractIn this study, the influence of direct electric current on a microcapsule was investigated. The microcapsule consisted of a core from a calcium ion and sodium alginate (SA) complex and the microcapsule membrane was formed by the polyionic complexation of poly(methylene-co-guanidine) (PMCG) and cellulose sulfate (CS). Microcapsules showed swelling and decreasing mechanical properties under the applied electric current, and the microcapsule membrane showed anisotropic swelling on the electrode side. The effect is attributed to an electrokinetic phenomenon, predominant formation of hydroxyl ions, and the diffusion of hydrated ions. The swelling degree of the microcapsule and microcapsule membrane at different pH and the applied electric current under alkali and acidic conditions was investigated. The swelling degree was influenced by the dissociation of the membrane, which was observed after applying the electric field, which was caused by the electrokinetic effect and the neutralization of the polycation (under alkali conditions) or polyanionic (under acidic conditions) segment during membrane formation.
Paralleling insulated-gate bipolar transistors in the H-bridge structure to reduce current stress
Authors: Memarian Sorkhabi Majid , Wendt Karen , Rogers Daniel , Denison Timothy 
Published: March 2021

AbstractIn this study we present the new power electronic circuit implementation to create the arbitrary near-rectangular electromagnetic pulse. To this end, we develop a parallel- Insulated-gate bipolar transistors (IGBT)-based magnetic pulse generator utilizing the H-bridge architecture. This approach effectively reduces the current stress on the power switches while maintaining a simple structure using a single DC source and energy storage capacitor. Experimental results from the circuit characterization show that the proposed circuit is capable of repeatedly generating near-rectangular magnetic pulses and enables the generation of configurable and stable magnetic pulses without causing excessive device stresses. The introduced device enables the production of near-rectangular pulse trains for modulated magnetic stimuli. The maximum positive pulse width in the proposed neurostimulator is up to 600 µs, which is adjustable by the operator at the step resolution of 10 µs. The maximum transferred energy to the treatment coil was measured to be 100.4 J. The proposed transcranial magnetic stimulator (TMS) device enables more flexible magnetic stimulus shaping by H-bridge architecture and parallel IGBTs, which can effectively mitigate the current stress on power switches for repetitive treatment protocols.
Pareto-improving transition to fully funded pensions under myopia
Authors: Andersen Torben M. , Bhattacharya Joydeep , Gestsson Marias H. 
Published: March 2021

AbstractUnder dynamic efficiency, a pay-as-you-go (PAYG) pension scheme helps the current generation of retirees but hurts future generations because they are forced to saveviaa return-dominated scheme. Abandoning it is deemed welfare-improving but typically not for all generations. But what if agents are present-biased (hence, undersave for retirement) and the “paternalistically motivated forced savings” component of a PAYG scheme motivated its existence in the first place? This paper shows it is possible to transition from such a PAYG scheme on to a higher return, mandated fully-funded scheme; yet, no generation is hurt in the process. The results inform the debate on policy design of pension systems as more and more policy makers push for the transition to take place but are forced to recognize that current retirees may get hurt along the way.
Overconvergent modular forms are highest-weight vectors in the Hodge-Tate weight zero part of completed cohomology
Authors: Howe Sean 
Published: March 2021

AbstractWe construct a$(\mathfrak {gl}_2, B(\mathbb {Q}_p))$and Hecke-equivariant cup product pairing between overconvergent modular forms and the local cohomology at$0$of a sheaf on$\mathbb {P}^1$, landing in the compactly supported completed$\mathbb {C}_p$-cohomology of the modular curve. The local cohomology group is a highest-weight Verma module, and the cup product is non-trivial on a highest-weight vector for any overconvergent modular form of infinitesimal weight not equal to$1$. For classical weight$k\geq 2$, the Verma has an algebraic quotient$H^1(\mathbb {P}^1, \mathcal {O}(-k))$, and on classical forms, the pairing factors through this quotient, giving a geometric description of ‘half’ of the locally algebraic vectors in completed cohomology; the other half is described by a pairing with the roles of$H^1$and$H^0$reversed between the modular curve and$\mathbb {P}^1$. Under minor assumptions, we deduce a conjecture of Gouvea on the Hodge-Tate-Sen weights of Galois representations attached to overconvergent modular forms. Our main results are essentially a strict subset of those obtained independently by Lue Pan, but the perspective here is different, and the proofs are short and use simple tools: a Mayer-Vietoris cover, a cup product, and a boundary map in group cohomology.
Conserving the Critically Endangered tree Vatica cauliflora: population and conservation status assessment
Authors: Robiansyah Iyan , Primananda Enggal , Rinandio Dipta Sumeru , Linsky Jean 
Published: March 2021

Abstract
Vatica cauliflora P.S. Ashton (Dipterocarpaceae) is a threatened tree species endemic to Kapuas Hulu District, West Kalimantan Province, Indonesia. The species is only known from the type specimen collected in 1953. After this first collection, there was no record confirming the presence of this tree in its natural habitat. Our recent surveys in 2019 and 2020 located 179 individuals of the species in six unprotected locations. The population's size structure is dominated (62.6%) by young individuals within the 0–5 cm diameter class. Our surveys also showed that the habitat of V. cauliflora is degraded as a result of the negative effects of agriculture and logging. Assessment with the IUCN Red List criteria indicates that V. cauliflora should be categorized as Critically Endangered.
N-terminal pro-B-type-natriuretic peptide as a screening tool for pulmonary hypertension in the paediatric population
Authors: Dasgupta Soham , Bettermann Erika , Kelleman Michael , Kanaan Usama , Sachdeva Ritu , Petit Christopher , Kim Dennis , Vincent Robert , Bauser-Heaton Holly 
Published: March 2021

AbstractBackground:Although cardiac catheterisation (cath) is the diagnostic test for pulmonary hypertension, it is an invasive procedure. Echocardiography (echo) is commonly used for the non-invasive diagnosis of pulmonary hypertension but maybe limited by lack of adequate signals. Therefore, emphasis has been placed on biomarkers as a potential diagnostic tool. No prior paediatric studies have simultaneously compared N-terminal pro-B-type-natriuretic peptide (NTproBNP) with cath/echo as a potential diagnostic tool. The aim of this study was to determine if NTproBNP was a reliable diagnostic tool for pulmonary hypertension in this population.Methods:Patients were divided into Study (echo evidence/established diagnosis of pulmonary hypertension undergoing cath) and Control (cath for small atrial septal defect/patent ductus arteriosus and endomyocardial biopsy post cardiac transplant) groups. NTproBNP, cath/echo data were obtained.Results:Thirty-one patients met inclusion criteria (10 Study, 21 Control). Median NTproBNP was significantly higher in the Study group. Echo parameters including transannular plane systolic excursion z scores, pulmonary artery acceleration time and right ventricular fractional area change were lower in the Study group and correlated negatively with NTproBNP. Receiver operation characteristic curve analysis demonstrated NTproBNP > 389 pg/ml was 87% specific for the diagnosis of pulmonary hypertension with the addition of pulmonary artery acceleration time improving the specificity.Conclusions:NTproBNP may be a valuable adjunctive diagnostic tool for pulmonary hypertension in the paediatric population. Echo measures of transannular plane systolic excursion z score, pulmonary artery acceleration time and right ventricular fractional area change had negative correlations with NTproBNP. The utility of NTproBNP as a screening tool for pulmonary hypertension requires validation in a population with unknown pulmonary hypertension status.