bluuebunny/crossref_metadata_2025_split huggingface dataset:
The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6
Authors: Oelze Beatrice , Elger Kirsten , Schadzek Patrik , Burmeister Laura , Hamm Anika , Laggies Sandra , Seiffart Virginia , Gross Gerhard , Hoffmann Andrea 
Published: May 2021

AbstractAs a response to pro-inflammatory signals mesenchymal stem cells (MSCs) secrete agents and factors leading to lymphocyte recruitment, counteracting inflammation, and stimulating immunosuppression. On a molecular level, the signalling mediator TGF-β-activated kinase 1 (TAK1) is activated by many pro-inflammatory signals, plays a critical role in inflammation and regulates innate and adaptive immune responses as well. While the role of TAK1 as a signalling factor promoting inflammation is well documented, we also considered a role for TAK1 in anti-inflammatory actions exerted by activated MSCs. We, therefore, investigated the capacity of lipopolysaccharide (LPS)-treated murine MSCs with lentivirally modulated TAK1 expression levels to recruit lymphocytes. TAK1 downregulated by lentiviral vectors expressing TAK1 shRNA in murine MSCs interfered with the capacity of murine MSCs to chemoattract lymphocytes, indeed. Analysing a pool of 84 secreted factors we found that among 26 secreted cytokines/factors TAK1 regulated expression of one cytokine in LPS-activated murine MSCs in particular: interleukin-6 (IL-6). IL-6 in LPS-treated MSCs was responsible for lymphocyte recruitment as substantiated by neutralizing antibodies. Our studies, therefore, suggest that in LPS-treated murine MSCs the inflammatory signalling mediator TAK1 may exert anti-inflammatory properties via IL-6.
Feasibility of a combined supervised and home-based whole-body vibration intervention in children after inpatient oncological treatment
Authors: Daeggelmann J. , Otten S. , Prokop A. , Maas V. , Streckmann F. , Bloch W. , Oschwald V. 
Published: May 2021

Abstract
Purpose
Reduced physical performance due to therapy-related dysfunctions in children diagnosed with cancer contributes to insufficient physical activity levels. It is therefore essential that relevant functions are restored. Whole-body vibration (WBV) training, a neuromuscular stimulating exercise intervention, could have the potential to target those functions adequately. Therefore, the aim of this study was to evaluate the feasibility of a combined supervised and home-based WBV intervention with children after inpatient oncological treatment.

Methods
Eight children aged 6–21 years were included after cessation of their inpatient oncological treatment. They performed a 12-week WBV training including one supervised and two home-based sessions per week according to a standardized training protocol. Feasibility, adherence and compliance to the vibration protocol were documented. Subjective benefits and satisfaction were assessed using a questionnaire.

Results
Study participants attended 84.03 ± 13.09% of WBV sessions. No serious adverse events occurred. Some patients reported side effects partially resulting in modifications of the vibration protocol by therapists. Almost all children regarded the intervention as beneficial.

Conclusion
A combined supervised and home-based WBV intervention with children after inpatient oncological treatment is feasible and safe. The beneficial potential regarding relevant sensory and motor dysfunctions is now to be investigated.

Trial registration number and date of registration
DRKS00014713 15.05.2018
Semen quality as a potential susceptibility indicator to SARS-CoV-2 insults in polluted areas
Authors: Montano Luigi , Donato Francesco , Bianco Pietro Massimiliano , Lettieri Gennaro , Guglielmino Antonino , Motta Oriana , Bonapace Ian Marc , Piscopo Marina 
Published: May 2021

AbstractThe epidemic of the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted worldwide with its infectious spread and mortality rate. Thousands of articles have been published to tackle this crisis and many of these have indicated that high air pollution levels may be a contributing factor to high outbreak rates of COVID-19. Atmospheric pollutants, indeed, producing oxidative stress, inflammation, immuno-unbalance, and systemic coagulation, may be a possible significant co-factor of further damage, rendering the body prone to infections by a variety of pathogens, including viruses. Spermatozoa are extremely responsive to prooxidative effects produced by environmental pollutants and may serve as a powerful alert that signals the extent that environmental pressure, in a specific area, is doing damage to humans. In order to improve our current knowledge on this topic, this review article summarizes the relevant current observations emphasizing the weight that environmental pollution has on the sensitivity of a given population to several diseases and how semen quality, may be a potential indicator of sensitivity for virus insults (including SARS-CoV-2) in high polluted areas, and help to predict the risk for harmful effects of the SARS-CoV-2 epidemic. In addition, this review focused on the potential routes of virus transmission that may represent a population health risk and also identified the areas of critical importance that require urgent research to assess and manage the COVID-19 outbreak.
Orientation effect of electropolishing characteristics of 316L stainless steel fabricated by laser powder bed fusion
Authors: Han Wei , Fang Fengzhou 
Published: May 2021

Abstract3D metal printing process has attracted increasing attention in recent years due to advantages, such as flexibility and rapid prototyping. This study aims to investigate the orientation effect of electropolishing characteristics on different surfaces of 316L stainless steel fabricated by laser powder bed fusion (L-PBF), considering that the rough surface of 3D printed parts is a key factor limiting its applications in the industry. The electropolishing characteristics on the different surfaces corresponding to the building orientation in selective laser melting are studied. Experimental results show that electrolyte temperature has critical importance on the electropolishing, especially for the vertical direction to the layering plane. The finish of electropolished surfaces is affected by the defects generated during L-PBF process. Thus, the electropolished vertical surface has higher surface roughness Sa than the horizontal surface. The X-ray photoelectron spectroscopy spectra show that the electropolished horizontal surface has higher Cr/Fe element ratio than the vertical surface. The electropolished horizontal surface presents higher corrosion resistance than the vertical surface by measuring the anodic polarization curves and fitting the equivalent circuit of experimental electrochemical impedance spectroscopy.
The size–growth relationship in the social services sector in Austria
Authors: Reitzinger Stephanie , Pennerstorfer Astrid 
Published: May 2021

AbstractThe social services sector is among the fastest-growing industries, but it has gained little attention in the debate regarding firm growth. This article analyzes firm growth in relation to firm size using payroll expenses as our indicator for both firm growth and firm size. We apply structural equation modeling and full maximum likelihood estimation using Austrian data comprising all non-profit social service providers. After 2013, fewer firms have been entering and more have been exiting the sector; however, we find that growing in size is still not associated with higher growth rates. Our study emphasizes the role of small organizations in remaining a growth sector.
An analysis of the cost of postgraduate training in surgery in Ireland compared to other specialties
Authors: H. Earley , K. Mealy 
Published: May 2021

Abstract
Introduction
Postgraduate specialty training in Ireland is associated with considerable cost. Some of these are mandatory costs such as medical council fees, while others are necessary to ensure career progression, such as attendance at courses and conferences. In particular, surgical specialities are believed to be associated with high training costs. It is unknown how these costs compare to those borne by counterparts in other specialities.

Aims
The aims of this study were to

Quantify the amount that trainees in Ireland spend on postgraduate training

Determine whether a difference exists between surgery and other non-skill-based specialties in terms of expenditure on training

Methods
A standardised non-mandatory questionnaire was circulated to trainees across two training centres in Ireland. Trainees at all levels were invited to participate.

Results
Sixty responses were obtained. Fifty-seven questionnaires were fully completed and included for analysis. The median expenditure on training was higher for surgical than non-surgical specialities. Subgroup analysis revealed surgical training was associated with higher expenditure on higher degrees and courses compared to medical training (p = 0.035). > 95% of trainees surveyed felt that greater financial support should be available for trainees during the course of their training.

Conclusions
This study demonstrated that a career in surgery is associated with higher ongoing costs for higher degrees and courses than counterparts in non-surgical training. All surgical trainees surveyed felt that better financial support should be available. Increasing financial support for may be a tangible way to mitigate against attrition during training.
The role of unconventional T cells in COVID-19
Authors: Orumaa Kristen , Dunne Margaret R. 
Published: May 2021

AbstractCOVID-19 is a respiratory disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It was first documented in late 2019, but within months, a worldwide pandemic was declared due to the easily transmissible nature of the virus. Research to date on the immune response to SARS-CoV-2 has focused largely on conventional B and T lymphocytes. This review examines the emerging role of unconventional T cell subsets, including γδ T cells, invariant natural killer T (iNKT) cells and mucosal associated invariant T (MAIT) cells in human SARS-CoV-2 infection.Some of these T cell subsets have been shown to play protective roles in anti-viral immunity by suppressing viral replication and opsonising virions of SARS-CoV. Here, we explore whether unconventional T cells play a protective role in SARS-CoV-2 infection as well. Unconventional T cells are already under investigation as cell-based immunotherapies for cancer. We discuss the potential use of these cells as therapeutic agents in the COVID-19 setting. Due to the rapidly evolving situation presented by COVID-19, there is an urgent need to understand the pathogenesis of this disease and the mechanisms underlying its immune response. Through this, we may be able to better help those with severe cases and lower the mortality rate by devising more effective vaccines and novel treatment strategies.
Roles of Immune Cells in Hereditary Angioedema
Authors: Ferrara Anne Lise , Cristinziano Leonardo , Petraroli Angelica , Bova Maria , Gigliotti Maria Celeste , Marcella Simone , Modestino Luca , Varricchi Gilda , Braile Mariantonia , Galdiero Maria Rosaria , Spadaro Giuseppe , Loffredo Stefania 
Published: May 2021

AbstractHereditary angioedema (HAE) is a rare genetic disease, characterized by recurrent and unexpected potentially life-threatening mucosal swelling. HAE may be further classified into HAE with C1‐inhibitor deficiency (C1‐INH‐HAE) and HAE with normal C1‐INH activity (nlC1‐INH‐HAE), mostly due to mutations leading to increased vascular permeability. Recent evidence implicates also the innate and adaptive immune responses in several aspects of angioedema pathophysiology. Monocytes/macrophages, granulocytes, lymphocytes, and mast cells contribute directly or indirectly to the pathophysiology of angioedema. Immune cells are a source of vasoactive mediators, including bradykinin, histamine, complement components, or vasoactive mediators, whose concentrations or activities are altered in both attacks and remissions of HAE. In turn, through the expression of various receptors, these cells are also activated by a plethora of molecules. Thereby, activated immune cells are the source of molecules in the context of HAE, and on the other hand, increased levels of certain mediators can, in turn, activate immune cells through the engagement of specific surface receptors and contribute to vascular endothelial processes that lead to hyperpemeability and tissue edema. In this review, we summarize recent developments in the putative involvement of the innate and adaptive immune system of angioedema.
Stress testing supply chains and creating viable ecosystems
Authors: Ivanov Dmitry , Dolgui Alexandre 
Published: May 2021

AbstractBusinesses and governments are becoming increasingly concerned about the resilience of supply chains and calling for their review and stress testing. In this conceptual essay, we theorize a human-centred ecosystem viability perspective that spans the dimensions of resilience and sustainability and can be used as guidance for the conceptualization of supply chain resilience analysis in the presence of long-term crises. Subsequently, we turn to the technological level and present the digital supply chain twin as a contemporary instrument for stress testing supply chain resilience. We provide some implementation guidelines and emphasize that although resilience assessment of individual supply chains is important and critical for firms, viability analysis of intertwined supply networks and ecosystems represents a novel and impactful research perspective. One of the major outcomes of this essay is the conceptualization of a human-centred ecosystem viability perspective on supply chain resilience.
Effects of Heat Stress on Gut Microbiome in Rats
Authors: Qu Qian , Li Hua , Bai Lin , Zhang Shiwei , Sun Jiaqi , Lv Weijie , Ye Chunxin , Liu Cui , Shi Dayou 
Published: May 2021

AbstractGut microbiome, as the largest and most important micro-ecosystem, plays a critical role in health. The purpose of this study was to evaluate whether heat stress modulates the composition and diversity of the gut microbiome in rats. The heat stress model was prepared in rats with the heating temperature maintained at 35–38°C. Cecum contents were collected after heat stress for 3 h and days 1, 3 and 7. Total DNA was extracted for 16 S rRNA sequencing and analysis of intestinal microbiome composition and diversity. The study showed that the composition of the intestinal microbiome of heat stress group was changed. And the heat stress modulated key phylotypes of gut microbiota at the level of phylum and genus. In particular, the genus of Lactobacillus and Bacteroides were significantly reduced, whereas the Oscillospira and Clostridium were increased by heat stress. Meanwhile, the rats under the heat stress encountered the change in carbohydrate metabolism, amino acid metabolism, and membrane transport to defense against stress. Taken together, the composition and structure of gut microbiome were affected by heat stress and some key phylotypes were also significantly altered. We conclude that the heat stress could impact multiple biological functions, via altering the gut microbiome.
UBCG2: Up-to-date bacterial core genes and pipeline for phylogenomic analysis
Authors: Kim Jihyeon , Na Seong-In , Kim Dongwook , Chun Jongsik 
Published: May 2021

AbstractPhylogenomic tree reconstruction has recently become a routine and critical task to elucidate the evolutionary relationships among bacterial species. The most widely used method utilizes the concatenated core genes, universally present in a single-copy throughout the bacterial domain. In our previous study, a bioinformatics pipeline termed Up-to-date Bacterial Core Genes (UBCG) was developed with a set of bacterial core genes selected from 1,429 species covering 28 phyla. In this study, we revised a new bacterial core gene set, named UBCG2, that was selected from the more extensive genome sequence set belonging to 3,508 species spanning 43 phyla. UBCG2 comprises 81 genes with nine Clusters of Orthologous Groups of proteins (COGs) functional categories. The new gene set and complete pipeline are available at http://leb.snu.ac.kr/ubcg2.
The representativeness of the dental calculus dietary record: insights from Taï chimpanzee faecal phytoliths
Authors: Power Robert C. , Wittig Roman M. , Stone Jeffery R. , Kupczik Kornelius , Schulz-Kornas Ellen 
Published: May 2021

AbstractIn recent years, new applications of microremain dietary analysis using dental calculus as a source of dietary data on ancient human subsistence and behaviours have accelerated. The dental calculus of contemporary human and non-human populations with known diets have been used as reference datasets, including the chimpanzees of Taï National Park (Côte d'Ivoire), but explaining the preservation mechanism involved is challenged by our incomplete knowledge of the microremain content within the diets of these reference populations and our rudimentary information on microremain incorporation into dental calculus. Here, we analyse phytoliths in faecal samples to assess to what extent plant phytoliths of a diet are reflected in the dental calculus as well as in the egested faeces. In this study, we identify and document the faecal phytolith assemblages as an indicator of plant consumption in two Western chimpanzees of the Taï National Park (Côte d'Ivoire) before (wet season), during (dry season) and after (dry season) a dust-rich period. Moreover, observational dietary records of these two individuals were compiled to improve the interpretability of this dental calculus phytolith dataset. The faecal phytolith assemblages vary significantly across samples in terms of abundance and diversity. The most common phytolith morphotypes were eudicot plates, single-cell and multi-cell tracheids, monocot rugulose and echinate spheroids and, to a lesser extent, unspecified thick and thin elongates. High loads of grit and other micro-remains (e.g. diatoms) are found during the dry period. Using observational dietary records as a starting point and our faecal results as a terminus, we consider how dental calculus can accumulate phytoliths. Our findings enable identification of the phytolith morphotypes that are under-represented in dental calculus, which is highly informative for future dental calculus research strategies.
Demographic and territorial characteristics of COVID-19 cases and excess mortality in the European Union during the first wave
Authors: Goujon Anne , Natale Fabrizio , Ghio Daniela , Conte Alessandra 
Published: May 2021

AbstractThis article explores for a large number of countries in the European Union (plus the United Kingdom) the main demographic differentials in positive tested COVID-19 cases and excess mortality during the first wave in 2020, accounting for differences at territorial level, where population density and size play a main role in the diffusion and effects of the disease in terms of morbidity and mortality. This knowledge complements and refines the epidemiological information about the spread and impact of the virus. For this analysis, we rely on the descriptive exploration of (1) data from The European Surveillance System (TESSy) database developed at the European Centre for Disease Prevention and Control (ECDC) on the number of cases and fatality rates and (2) of weekly mortality data collected by Eurostat. The analysis at territorial level studies the changes in R0—the basic reproduction number—and median excess mortality, across territories with different levels of urbanization. The unique findings of this study encompassing most European Union Member States confirm and define the demographic and territorial differential impacts in terms of infections and fatalities during the first wave of the pandemic in 2020. The information is important for stakeholders at European Union, national and sub-national levels in charge of designing containment measures for COVID-19 and adaptation policies for the future by anticipating the rebound for certain segments of the population with differential medical and economic needs.
Hydrochemistry and environmental isotopes (18O, 2H, 3H, 3He/4He) of groundwater and floodwater in the great area of Hurghada, Eastern Desert of Egypt
Authors: Wannous M. , Jahnke C. , Troeger U. , Falk M. , Bauer F. 
Published: May 2021

AbstractPorous and fractured aquifers exist in the area of Hurghada, Eastern Desert of Egypt, whose recharge processes through the common flash floods are not identified. Hydrochemical parameters, stable isotopes 18O, 2H and tritium in floodwater and groundwater were applied in the area subject to study. Additionally, He isotopes were investigated in the deep wells in the faulted zone at the Abu Shaar Plateau. 3H activity in all sampled points lies below the detection limit excluding a recent recharge component in groundwater. However, the hydrochemical ratios and the stable isotope signature confirm that the shallow wells and springs (Red Sea Hills group) are being recharged from modern precipitation. The hydrochemical parameters of the deep wells at the Abu Shaar Plateau (coastal plain group) confirm another origin for the ions rather than the modern precipitation. Together with the 18O and 2H values, the Br/Cl ratio of this group confirms the absence of seawater intrusion component and the role of the fault as a hydraulic barrier. These 18O and 2H values deviate from the GMWL confirming an evaporation effect and colder infiltration conditions and reveal strongly a possible mixing with the Nubian Sandstone in the region. The 3He/4He ratio confirms a mantle contribution of 2% from the total He components.
Metabolic reprogramming and epigenetic modifications on the path to cancer
Authors: Sun Linchong , Zhang Huafeng , Gao Ping 
Published: May 2021

AbstractMetabolic rewiring and epigenetic remodeling, which are closely linked and reciprocally regulate each other, are among the well-known cancer hallmarks. Recent evidence suggests that many metabolites serve as substrates or cofactors of chromatin-modifying enzymes as a consequence of the translocation or spatial regionalization of enzymes or metabolites. Various metabolic alterations and epigenetic modifications also reportedly drive immune escape or impede immunosurveillance within certain contexts, playing important roles in tumor progression. In this review, we focus on how metabolic reprogramming of tumor cells and immune cells reshapes epigenetic alterations, in particular the acetylation and methylation of histone proteins and DNA. We also discuss other eminent metabolic modifications such as, succinylation, hydroxybutyrylation, and lactylation, and update the current advances in metabolism- and epigenetic modification-based therapeutic prospects in cancer.