bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biop-C: A Method for Chromatin Interactome Analysis of Solid Cancer Needle Biopsy Samples
Authors: Animesh Sambhavi , Choudhary Ruchi , Ng Xin Yi , Tay Joshua Kai Xun , Chong Wan-Qin , Goh Boon Cher , Fullwood Melissa Jane 
Published: January 2021

AbstractA major challenge in understanding the 3D genome organization of cancer samples is the lack of a method adapted to solid cancer needle biopsy samples. Here we developed Biop-C, a modifiedin situHi-C method, and applied it to characterize three nasopharyngeal cancer patient samples. We identified Topologically-Associated Domains (TADs), chromatin interaction loops, and Frequently Interacting regions (FIREs) at key oncogenes in nasopharyngeal cancer from Biop-C heat maps. Our results demonstrate the utility of our Biop-C method in investigating the 3D genome organization in solid cancers, and the importance of 3D genome organization in regulating oncogenes in nasopharyngeal cancer.
Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits
Authors: Pieters Pascal A. , Nathalia Bryan L. , van der Linden Ardjan J. , Yin Peng , Kim Jongmin , Huck Wilhelm T.S. , de Greef Tom F. A. 
Published: January 2021

AbstractRegulatory pathways inside living cells employ feed-forward architectures to fulfill essential signal processing functions that aide in the interpretation of various types of inputs through noise-filtering, fold-change detection and adaptation. Although it has been demonstrated computationally that a coherent feed-forward loop (CFFL) can function as noise filter, a property essential to decoding complex temporal signals, this motif has not been extensively characterized experimentally or integrated into larger networks. Here we use post-transcriptional regulation to implement and characterize a synthetic CFFL in anEscherichia colicell-free transcription-translation system and build larger composite feed-forward architectures. We employ microfluidic flow reactors to probe the response of the CFFL circuit using both persistent and short, noise-like inputs and analyze the influence of different circuit components on the steady-state and dynamics of the output. We demonstrate that our synthetic CFFL implementation can reliably repress background activity compared to a reference circuit, but displays low potential as a temporal filter, and validate these findings using a computational model. Our results offer practical insight into the putative noise-filtering behavior of CFFLs and show that this motif can be used to mitigate leakage and increase the fold-change of the output of synthetic genetic circuits.
Real-Time Three-Dimensional Tracking of Single Vesicles Reveals the Abnormal Motion and Vesicle Pools of Synaptic Vesicles in Neurons of Huntington’s Disease Mice
Authors: Chen Sidong , Yoo Hanna , Li Chun Hei , Park Chungwon , Tan Li Yang , Jung Sangyong , Park Hyokeun 
Published: January 2021

SummaryAlthough defective synaptic transmission was suggested to play a role in neurodegenerative diseases, the dynamics and vesicle pools of synaptic vesicles during neurodegeneration remain elusive. Here, we performed real-time three-dimensional tracking of single synaptic vesicles in cortical neurons from a mouse model of Huntington’s disease (HD). Vesicles in HD neurons had a larger net displacement and radius of gyration compared with wild-type neurons. Vesicles with a high release probability (Pr) were interspersed with low-Prvesicles in HD neurons, whereas high-Prand low-Prvesicle pools were spatially separated in wild-type neurons. Non-releasing vesicles in HD neurons had an abnormally high prevalence of irregular oscillatory motion. These abnormal dynamics and vesicle pools were rescued by overexpressing Rab11, and the abnormal irregular motion was rescued by jasplakinolide. These results suggest the abnormal dynamics and vesicle pools of synaptic vesicles in the early stages of HD, suggesting a possible pathogenic mechanism of neurodegenerative diseases.
Is earlier reproduction associated with higher or lower survival? Antagonistic results between individual and population scales in the blue tit
Authors: Bastianelli Olivier , Charmantier Anne , Biard Clotilde , Bonamour Suzanne , Teplitsky Céline , Robert Alexandre 
Published: January 2021

ABSTRACTAlthough it has been shown that phenology can respond to temporal environmental variation in free ranging populations of several species, little is known about the mechanisms of these responses and their effects on demography, and in particular on survival. Exploring phenological responses and their associated consequences on survival can be achieved at two distinct scales: the population scale, which focusses on a set of common responses to environmental conditions, and the individual scale, focusing on the relative position of each individual in the distribution of survival and phenology under particular conditions. In this study, we apply capture-mark-recapture multistate modelling on a 38-year monitoring dataset of blue tits (Cyanistes caeruleus) to investigate the effects of breeding phenology and some demographic covariates (breeding density, average and individual breeding success) on adult survival, at both population and individual scales. Our analysis revealed that (i) at the population scale, early breeding years are followed by lower average adult survival. (ii) At the individual level, earlier breeders within the population have higher subsequent survival than later breeders, although this relationship is reversed in years with very harsh conditions, e.g. warm spring and high breeding density. (iii) High individual relative breeding success is also associated with higher subsequent survival and explains more survival variation than relative phenology. Overall, our study indicates that, although earlier breeding is associated with a survival cost at the population level, substantial intrapopulation hererogeneity shapes a positive association between earlier breeding, breeding success and survival at the individual level.
anonymizeBAM: Versatile anonymization of human sequence data for open data sharing
Authors: Ziegenhain Christoph , Sandberg Rickard 
Published: January 2021

AbstractThe risks associated with re-identification of human genetic data are severely limiting open data sharing in life sciences. Here, we developedanonymizeBAM, a versatile tool for the anonymization of genetic variant information present in sequence data. ApplyinganonymizeBAMto single-cell RNA-seq and ATAC-seq datasets confirmed the complete removal of donor-related genetic information. Therefore, the accurate generation of de-identified sequence data will re-enable open sharing in sequencing-based studies for improved transparency, reproducibility, and innovation.
Corporate reporting to the crown: a longitudinal case from colonial Africa
Authors: Power Sean Bradley , Brennan Niamh M. 
Published: January 2021

PurposeA royal charter of incorporation imposing public benefit/social responsibilities established the privately owned British South Africa Company (BSAC), in return for power to exploit a huge territory using low-cost local labour. This study explores the dual principal–agent problem of how the BSAC used annual report narratives to report on its conflicting economic responsibilities to investors versus its public benefit charter responsibilities to the British Crown.Design/methodology/approachHaving digitised the dataset, the research analyses narratives from 29 BSAC annual reports spanning a continuous 35-year royal charter period, using computer-aided keyword content analysis to identify economic-orientated versus public benefit-orientated annual report narratives. The research analyses how the annual report narratives shifted according to four key contextual periods by reference to the changing influence of private investors versus the British Crown.FindingsThere are two key findings. First, economic primacy. At no point do public benefit disclosures outweigh economic disclosures. Second, the BSAC's meso-corporate context and macro-social/political context can explain patterns in public benefit disclosures. The motivation for producing public benefit information is not altruism. Rather, commercial interests motivate disclosure. The BSAC used its annual reports to sustain what proved ultimately unsustainable – royal charter-style colonialism.Originality/valueThis accounting history study contributes to an understanding of corporate narrative reporting using one of the earliest known cases of such analysis and shows how accounting plays a central role in facilitating a company in sustaining its interests. This 100-year lookback may be a portend of the future for modern-day annual report corporate social responsibility narratives in, say, mining and oil and gas company corporate reports, especially if these natural resources run out.
Social integration through social connection in everyday life. Residents' experiences during the COVID-19 pandemic in SällBo collaborative housing, Sweden
Authors: Arroyo Ivette , Montesino Norma , Johansson Erik , Yahia Moohammed Wasim 
Published: January 2021

PurposeThe aim of this article is to explore the everyday life experiences of elderly (+70 years) living with young locals and refugees in a collaborative housing project before and during the COVID-19 pandemic in Sweden. The paper discusses the importance of the spatial dimension in the conceptualization of social integration.Design/methodology/approachThe main method is a qualitative case study based on observations of settings, document/video analysis, online diary entries made by ten residents and eight semi- structured interviews conducted with the residents.FindingsSällBo was conceived as a new type of collaborative housing in which elderly, young locals and refugees share common spaces with the aim of enabling social integration. In this context, COVID-19 interrupted the ongoing processes of living together after four months of moving to the house. The three main themes that emerge from the empirical material are (1) changes in the use of common spaces and social interactions, (2) residents' resilient coping responses during the pandemic and (3) insights for future design of collaborative housing based on their experience. The pandemic caused a moment of institutional vacuum, which triggered the agency of the residents whilst developing social bonds and social bridges among them.Social implicationsSocial connection created in everyday life at SällBo's common spaces has triggered processes of social integration.Originality/valueThe ongoing processes of social integration have included the spatial dimension. We understand social integration as a process that involves people from different generations and ethnical backgrounds, which takes place in common spaces and everyday life as different modes of socialization.
Customer process-centric artefacts: a literature review
Authors: Palvölgyi Elisabeth Zsoka , Moormann Jürgen 
Published: January 2021

PurposeCompanies that strive to provide customers with value in their processes benefit from artefacts that allow them to better understand customer processes (CPs) and to influence CPs in ways that are valuable for customers. Such CP-centric artefacts (CPCAs) carry various labels across research fields, which inhibit their application or utilization for developing further artefacts. This study provides a structured overview of existing CPCAs and investigates which research foundations promote their development.Design/methodology/approachTwenty-five CP-related keyword combinations are applied in several iterations using multiple (meta) search engines to identify papers on CPCAs across different research streams. Introduced research frameworks organize the identified artefacts and indicate research gaps and reasons why some approaches are more successful in developing CPCAs than others.FindingsExisting CPCAs cover different aspects of CPs and utilize contextual factors of CPs to varying degrees to analyse or influence CPs. Research gaps are identified that indicate opportunities to develop further CPCAs. Taking instantiated methods in combination with CP-related descriptive knowledge as a foundation yields the highest potential for generating beneficial CPCAs.Originality/valueThis paper contributes to CP management literature by offering a foundation for the generation of CPCAs suitable for analysing and influencing CPs of end-consumers. This supports the establishment of a CP management aiming at optimizing both, interlinked business processes and CPs.
Compact multiple electromagnetic band gap (EBG) cells based ultra wide band (UWB) filter with improved stop-band characteristics
Authors: Molimoli Hajamohideen Masood , Balakrishanapillai Suseela Sreeja 
Published: January 2021

Purpose
The purpose of the study is – in Microwave filter design, the performances of passive components are deteriorated by parasitics at gigahertz (GHz) frequency range. A compact and multi-stack electromagnetic band gap (EBG) structure is proposed with improved stop band characteristics at GHz frequency range in this work. This paper proposes a new design for ultra wide band pass filter (resonator BPF) with periodically loaded one-dimensional EBG to achieve the harmonic suppression. This basic EBG structure is developed with combination of a signal strip and ground plane in the slotted section. The resonator BPF is loaded with one EBG, two EBG and three EBGs to improve the stop-band rejection.

Design/methodology/approach
The proposed filter is with multi-stack EBG cell for achieving good pass band and stop bands performance. Circuit model is analyzed in Section 2. Section 3 discuses band pass filter loaded with one EBG. In Sections 4 and 5, filter with two and three EBG loaded resonators are discussed, respectively. Section 6 is concluded with comparison of simulation and measured results.

Findings
The stop-band rejection is 20 dB, 40 dB and 50 dB, respectively, in the frequency range of 6 GHz to 20 GHz. The simulation analysis is carried out with advanced system design software. To validate the simulation results, proposed structure is fabricated, and results are found to be in good agreement.

Originality/value
This paper accounts for designing resonator BPF, which has slow wave pass band and stop band characteristics. Second and third harmonics are suppressed using multi-stack EBG. Various stacks with basic designs are proposed and improved results have been demonstrated which is open for future research.
ESG and corporate financial performance: the mediating role of green innovation: UK common law versus Germany civil law
Authors: Chouaibi Salim , Chouaibi Jamel , Rossi Matteo 
Published: January 2021

PurposeThe purpose of this paper is to investigate the direct and indirect links between environmental, social and governance (ESG) practices and financial performance using the mediate role of green innovation.Design/methodology/approachTo test the current study hypotheses, the authors applied linear regressions with a panel data using the Thomson Reuters ASSET4 and Bloomberg database from a sample of 115 UK and 90 Germany companies selected from the ESG index over the period 2005–2019.FindingsThe results show that the strengths ESG increase the firm value and the weaknesses decrease it. In addition, the authors find that green innovation fully mediates the relationship between ESG practices and financial performance in UK and Germany.Practical implicationsThe findings provide interesting implications to academics practitioners and regulators who are interested in discovering ESG score, financial performance and green innovation. The results also provide insights to regulators and the board of directors on future growth opportunities for the company and the country.Originality/valueThis study is unique in examining the mediation effect of green innovation on the relationship between ESG practices and financial performance.
Career adaptability and job outcomes: a moderated mediation model of proactivity and job content plateau in educational sector
Authors: Kaur Harleen , Kaur Rajpreet 
Published: January 2021

PurposeVery little research has examined how adaptivity, adaptability resources, adapting responses and adaptation results are interlinked with each other. The current research aims to investigate whether career adaptability influences job outcomes via job content plateau. Taking career construction theory (Savickas, 2005) as a base, the research model of this study posited that employee's favorable job outcomes, i.e. job satisfaction and performance depend upon their psychosocial meta-capacities (career adaptability) and job content plateau. Further, the study is the first to examine the moderating role of proactivity among career adaptability, job content plateau and job outcomes relationship.Design/methodology/approachIt is a two-wave longitudinal study, quantitative in nature and has collected data from 357 faculty members of Indian universities. The hypotheses have been empirically tested through the structural equation modeling technique.FindingsThe moderated mediation model was supported, and as predicted, (1) career adaptability was positively related to job outcomes and (2) the mediated relationship between career adaptability and job outcomes via content plateau was stronger for individuals with high levels of proactivity.Practical implicationsThe study encourages career management practitioners and counselors to integrate proactive behaviors and career adaptability into counseling techniques to equip clients with necessary skills and deal with unfavorable job experiences, thereby engendering favorable job outcomes.Originality/valueThe current study is the first to test the intervening effect of proactivity in career adaptability and job outcomes relationships via job content plateau.
Reinforcing each other: strategies to advance international interlibrary loan
Authors: Thompson Hilary H. , Munson Kurt , Harper James B. 
Published: January 2021

Purpose
The purpose of this paper is to share the results of the 2019 international interlending survey conducted by the American Library Association, Reference and User Services Association (RUSA), Sharing and Transforming Access to Resources Section (STARS) International Interlibrary Loan (ILL) Committee, the fourth such survey conducted by this group since 2007.

Design/methodology/approach
Committee members developed the survey instrument and collected data in Qualtrics. Questions from the 2015 and 2011 instruments were reused to support longitudinal data analysis, and new questions related to improving the sharing of resources across borders were added. The survey was distributed to libraries worldwide via multiple methods, and translations in six of the seven official International Federation of Library Associations and Institutions languages were provided.

Findings
Results reveal challenges to engaging in global resource sharing in the areas of discovery and request methods, policies and service models, copyright and licensing, delivery and payment. Strategies to overcome these challenges, as informed by both the survey results and recent literature, are shared. Reinforcing each other, these independently and organically developed solutions illustrate ways to further advance ILL.

Practical implications
The findings can aid ILL practitioners and the organizations to which they belong in improving the sharing of resources across borders. By optimizing this service, libraries can better support researchers in a globalized environment.

Originality/value
The 2019 RUSA STARS survey is the first survey on the topic of international ILL to offer multiple translations complimenting global distribution. It contributes to the literature by offering a broad and updated perspective on current international ILL practices, challenges impeding the sharing of library materials abroad and possible solutions.
Hotels' marketing mix responses at insecurity-stricken destinations: a study in the US–Mexico border
Authors: Cruz-Milán Oliver 
Published: January 2021

PurposeThe purpose of this paper is to investigate specific marketing mix activities and influencing factors in hotels coping with falling room demand derived from drug cartel-related risk and insecurity.Design/methodology/approachA case study research was carried out using semistructured interviews with key informants (hotel managers) in two neighboring destinations at the US–Mexico border, an area where criminal organizations' drug trafficking-related violence has impacted the hospitality industry.FindingsThe research identifies factors that are internal (market segment diversification, type of ownership, magnitude of investments) and external (tourism promotion organizations, media coverage, tourist flow volume) to the firms as they affect their marketing mix implementation.Research limitations/implicationsThe research developed a framework to better understand the use of marketing mix practices and influencing factors in criminal insecurity contexts, which could be further studied in other risk and conflict scenarios.Practical implicationsThe pricing and communication tactics are employed more intensively, while product-service and distribution channel actions are used to a lesser extent. Greater emphasis should be placed on product-service, distribution and market segment diversification.Social implicationsConsidering the positive impacts that tourism and hospitality businesses have on local communities, it is recommended that the hotel sector works together with government and industry associations to improve the safety and security at tourism destinations.Originality/valueThe research extends the extant knowledge in hospitality crisis management by investigating the full marketing mix tactics in hotels at destinations stricken by cartel-related organized crime, an understudied context in the literature.
Can we predict the likelihood of financial distress in companies from their corporate governance and borrowing?
Authors: Mariano Sara Sofia Gomes , Izadi Javad , Pratt Maurice 
Published: January 2021

PurposeThe purpose of this study is to investigate the impact of corporate governance structures on the likelihood of financial distress in UK listed companies. The paper examines the impact of borrowing and corporate governance structures on financial distress likelihood in UK companies.Design/methodology/approachThe study uses a quantitative approach with financial, governance and borrowing measures and data from 270 firm-observations between 2010 and 2018. The study analyses the impact of borrowing and corporate governance structures to indicate financial distress likelihood in British companies. Corporate governance variables such as ownership concentration, independence indicators, chief executive officer duality, director remuneration and corporate loans are considered, as well as the UK Corporate Governance Code.FindingsThe results indicate that companies with low ownership concentration and a low degree of independence are more likely to incur financial distress. Larger boards and better director remuneration can reduce financial distress likelihood and the existence of corporate loans can increase this likelihood. Empirical consideration of corporate borrowing is a new contribution to the literature.Originality/valueVariables are highlighted and aggregated that have not otherwise been studied together; the UK Corporate Governance Code’s main ideas are empirically supported; the study is useful for defining corporate governance structure strategies.