bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and detailed characterisation of the first sterigmatocystin hyperproducer mould strain in Hungary
Authors: Dobolyi Cs. , Inotai K. , Bata-Vidács I. , Sárkány D. , Csernus O. , Kocsubé S. , Tóth B. , Szekeres A. , Kukolya J. 
Published: March 2021

AbstractAspergillus strains were isolated from Hungarian mills in order to get information on the appearance of sterigmatocystin (ST) producing moulds, whose presence has never been demonstrated in Hungary. Fungal isolates were classified into nine morphotypes, sections Nigri, Nidulantes, Versicolores (two morphotypes), Circumdati, Flavi (two morphotypes), Clavati and Terrei by classical mycological assays. ST producing strains could be classified into section Versicolores. ST production of the isolates was assessed by liquid and solid phase growth experiments and compared to ST producing reference strains: Aspergillus pepii SzMC 22332, Aspergillus versicolor SzMC 22333, Aspergillus griseoaurantiacus SzMC 22334 and Aspergillus nidulans RDIT9.32. Four of our isolates marked as Km11, Km14, Km26 and Km31 showed ST production in liquid medium. ST production on solid phase corn grit substrate was measured after three weeks of incubation, and Km26 isolate proved to be the most prominent with a toxin concentration of 277.1 μg g−1, surpassing all reference strains. The toxin-producing ability of Km26 isolate was also tested in a field experiment, where corn was infected. By the end of the experiment, ST level of 19.56 μg kg−1 was measured in infected corn.Molecular taxonomic identification of the Km26 strain was performed using internal transcribed spacer (ITS), calmodulin and tubulin sequence analyses. Based on these studies, strain Km26 was identified as Aspergillus creber.Here we report that an ST-producing A. creber strain has appeared in Hungary, and the Km26 strain is the first known extreme ST-producing mould in this country. As a result of climate change, aflatoxin B1 producing Aspergillus flavus strains have appeared in Hungary in the last decade. As strain Km26 is the only A. creber isolate in Hungary so far, there is no sign of mass prevalence, and due to the lower temperature optimum of the species compared to A. flavus, its appearance is probably not related to climate change.
Clinical Efficacy of Follitropin Alfa in GnRH-Antagonist Protocols: A Prospective Observational Phase IV Study on the Use of Biosimilar Follitropin Alfa r-hFSH in Assisted Reproductive Technology in a Routine Care Setting
Authors: Griesinger Georg , Schill Thilo , Sator Michael , Schenk Michael , Krüssel Jan-Steffen 
Published: March 2021

Background: This phase IV routine care study evaluated ovarian responses when using a biosimilar follitropin alfa r-hFSH (Bemfola®) for controlled ovarian stimulation (COS) in women undergoing assisted reproductive technology (ART) treatment who were pituitary-suppressed with a gonadotrophin-releasing hormone (GnRH) antagonist.
Methods: This multicenter, prospective, non-comparative, non-interventional study (Germany/Austria) was conducted with 885 women (Mean age of 34.0±4.4 years) for whom COS with Bemfola® and GnRH-antagonist for pituitary suppression were applied as part of in vitro fertilization (IVF) treatment with/without intracytoplasmic sperm injection (ICSI) observing routine clinical-practice protocols. Primary endpoint was the number of retrieved cumulus-oocyte-complexes (COCs).
Results: Among 986 ART cycles, COS was given for 9.9±1.8 days (First-day r-hFSH dose of 220.7±68.9 IU; mean total dose of 2184.3±837.5 IU). It was revealed that 99.1% of cycles resulted in follicular puncture, with mean of 10.7±6.6 oocytes retrieved. Successful fertilization took place after IVF/ICSI in 93.8% of follicular punctures. Freeze-all was performed in 14.2% of cycles. Fresh embryo transfer was performed in 76.9% of cycles with follicular puncture; mean day of transfer was 3.5±1.3 and average number of transferred embryos was 1.76±0.50. Clinical pregnancy rate was 30.2% of embryo-transfer cycles and 23.4% of started cycles. Sixty-nine reports of ovarian hy-perstimulation syndrome (7.0% of started cycles) were documented.
Conclusion: COS with Bemfola® in GnRH-antagonist IVF/ICSI protocols in a routine care setting led to an appropriate ovarian response allowing oocyte retrieval in 99.1% of initiated cases.
Chromosomal Analysis of Cumulus Cells as a Future Predictor for Oocyte Aneuploidy: A Case Report
Authors: Gonzalez Xavier Viñals , Ahmed-Odia Rabi , Gupta Sioban Sen , Naja Roy Pascal , Arshad Falak , Serhal Paul , Saab Wael , Seshadri Srividya 
Published: March 2021

Background: Within the ovary, the optimal growth of the follicle, oocyte maturation and ovulation are highly conditioned by the two-way cross talk and interactions between the oocyte and the immediate somatic cells, known as cumulus cells (CCs). This biological communication between cell lines triggered the interest in the study of CCs as a biomarker of oocyte competence.
Case Presentation: The findings of a 45,X mosaic pattern on CCs from a female patient with unremarkable medical history are reported in this study. The patient came to the Centre for Reproductive and Genetic Health, London on 14th August 2019 for her first visit and the follow up procedures were done for her to determine underlying genetic status. For this purpose, four sources of DNA including CCs, blood lymphocytes, buccal cells and immature oocytes were analyzed in the present report.
Conclusion: In the present case study, the hypothesis of the female patient being mosaic 45,X was confirmed although the degree of mosaicism and whether this was affecting the germinal line could not be determined. In the event of the discovery of a cell line with an apparently abnormal genetic makeup, genetic counselling is important in order to understand the implications from somatic to germinal cells for patients exploring fertility journeys
Identification and Ranking of the Reasons for not Referring Patients from Level 1 to Level 2 through Electronic Referral System of Golestan Province in 2019
Authors: Kabir Mohammad Javad , Heidari Alireza , Khatirnamani Zahra , Beygom Kazemi Sakine , Honarvar Mohammad Reza , Badakhshan Abbas , Arefnia Serajaldin , Lotfi Mansoureh , Sedaghat Seyed Mehdi , Hosseini Seyed Mohammad , Kamangari Mehrdad , Dadjoo Mohammad , Gholami Masoumeh , Rafiei Narges , Eri Maryam 
Published: March 2021

Background: The public sector of the health care system provides justice and access for all people who need more specialized services through the referral system. If the challenges of the referral system are not identified and addressed promptly, the system will not be efficient and effective. The purpose of this study was to rank the reasons for non-referral of outpatient level 1 to level 2 in the electronic referral system of Golestan province in 2019.
Methods: In this descriptive and cross-sectional study, 431 outpatients participated from the pilot hospitals of the e-referral system in Golestan province, who did not refer to the level 2 specialist despite that an appointment was set by the system. The participants were selected using a stratified random sampling method. Data were collected using a questionnaire and analyzed by descriptive statistics and Friedman test in SPSS 23 software.
Results: The most important reasons for patients who were not referred from level 1 to level 2 in the electronic referral system included the presence of a trusted and familiar physician, inability to pay the visit of a specialist, and high cost of transportation from home to the designated specialist, respectively. The gender of the specialist physician and lack of guidance on referral to the specialist physician were indicated by the referral center. Patients ranked the reasons of not referring to the specialist differently (P < 0.001).
Conclusions: The current status can be improved by increasing the public confidence in specialized physicians working in the electronic referral system and making the specialized services free for the poor patients. Furthermore, raising the patient's awareness about using Level 2 specialized services through the mass media can help patients to benefit from these services.
Designing a Model for Managing the Food Service Department in Iranian Hospitals
Authors: Fathi Esa , Nasiripour Amir Ashkan , Khalesi Nader , Ziyari Reaz 
Published: March 2021

Background: Optimal management of food services in hospitals plays an important role in their performance, accelerating the patients’ recovery and increasing the level of patient satisfaction. The purpose of this study was to design a model for food service management in Iranian hospitals.
Methots: This descriptive correlational study was carried out using structural equation modeling. Simple random sampling was performed and the study participants were selected among hospital managers, nutrition experts, and environmental health experts in a hospital management conference in Tehran in September 2018. The measurement tool was a researcher-made questionnaire with confirmed construct validity and corroborated reliability through Cronbach's alpha. For data analysis, exploratory factor analysis and confirmatory factor analysis were performed via SPSS 21 and AMOS 22 software.
Results: Based on the obtained model, 5 main factors affecting the management of food service sector were identified, including: health and safety factors, satisfaction factors, food waste factors, monitoring and control factors, and mechanism factors. Among these, health and mechanism factors with coefficients of 0.874 and 0.612 had the highest and the lowest effect in the management of food services in Iranian hospitals, respectively.
Conclusion: According to the obtained model, various factors are effective in food management that should be considered in hospital planning. Based on this, the food health and safety dimensions are of particular importance.
The Impact of a Dedicated Nurse on the Prevention of Central Line-Associated Bloodstream Infections in the NICU
Authors: Curry Sabra , Mallard Ellen , Marrero Elizabeth , Walker Melinda , Weeks Robin , Harrison H. Allen , Temple Melany , Holland Cara , Brandon Katherine 
Published: March 2021

BackgroundThe neonatal population is at increased risk for central line-associated bloodstream infections (CLABSIs) related to prematurity, critical illness, and compromised immune function.1,4,5MethodsTo address a 30 percent CLABSI rate increase, a quality improvement (QI) project in a Level IV NICU was developed and implemented by the NICU CLABSI team in 2018. The project trialed a dedicated CLABSI prevention-registered nurse (DCP-RN) role with select responsibilities aimed at rate reduction. The DCP-RN spearheaded an RN education plan, addressed prevention bundle compliance, and aided in establishing a reliable apparent cause analysis (ACA) process.ResultsThe outcome resulted in an over 50% reduction in the CLABSI rate and permanent adoption of the DCP-RN role in the NICU.
An Integrated Simulation Approach for Wellbore Blockage Considering Precipitation, Aggregation, and Deposition of Asphaltene Particles
Authors: Qi Minhui , Moghanloo Rouzbeh Ghanbarnezhad , Su Xin , Li Mingzhong 
Published: March 2021

Summary
Asphaltene deposition triggers serious flow assurance issues and can significantly restrict the production capacity. Because of the complexity associated with asphaltene deposition that includes several mechanisms acting simultaneously, an accurate prediction of asphaltene blockage along the wellbore requires integration of asphaltene precipitation, aggregation, and deposition. In this work, an integrated simulation approach is proposed to predict the asphaltene deposition profile along the wellbore. The proposed approach is novel because it integrates various deposition patterns of particulate flow (which depends on hydrodynamics) with aggregation processes to investigate how the distribution of asphaltene particle size varies (governed by molecular dynamics) after being precipitated out of the oil phase (controlled by thermodynamics).
To improve the predictability capability of simulations, a direct input from the wellbore flow simulator is used to update the velocity profile after the wellbore radius changes beyond a certain predefined threshold. The fraction of asphaltene precipitation is determined using the asphaltene solubility model and combined with aggregation models to feed into deposition calculations.
Wellbore blockage was examined for two cases with and without the aggregation mechanism included. A sensitivity analysis was carried out to study parameters that affect the severity of blockage, such as range of pressure-temperature along the wellbore, flow velocity, and radial distribution of asphaltene particles. The simulation approach proposed in this paper provides an in-depth understanding of the wellbore flow assurance issues caused by asphaltene deposition and thus provides useful insights for improving the predictions of production performance.
Intentional Fallers with Complex Pelvic and Acetabular Fractures Do Not have Worse Radiological and Functional Outcomes than Accidental Fallers
Authors: Yu Yi-Hsun , Chou Ying-Chao , Hsu Yung-Heng , Chen I-Jung , Wei Lien-Chung 
Published: March 2021

Abstract
Background: Individuals who fell from heights of >6 m accidentally or intentionally can suffer from complex pelvic and acetabular fractures. The extent to which an intentional fall correlates with prognosis and outcome after osteosynthesis is unclear. We aimed to investigate the clinical outcomes of fallers with pelvic and acetabular fractures after osteosynthesis and to compare the radiological and functional outcomes between intentional and accidental fallers.Methods: Between 2014 and 2017, individuals who fell from heights of >6 m, developed pelvic and acetabular fractures, survived after resuscitation, and completed surgical treatments were enrolled. The fallers were divided into the intentional and accidental fallers, and their clinical parameters were statistically evaluated and compared.Results: Forty-nine fallers who underwent osteosynthesis for pelvic and acetabular fractures were included. Sixteen patients were intentional fallers, whereas the rest of the patients fell accidentally. All patients who fell intentionally had pre-existing mental disorders, and the major diagnosis was adjustment disorder. The group with intentional fallers had female sex predominance, higher injury and new injury severity scores, and longer hospital stay. However, the early loss of fixation (less than three month) and functional outcomes (6- and 12-month follow-up) did not significantly differ between the intentional and accidental fallers. Conclusions: The intentional fallers with pelvic and acetabular fractures might be accompanied by more severe injuries compared to accidental fallers. However, the radiological and functional outcomes of the intentional fallers after osteosynthesis were not inferior to those of the accidental fallers.
Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea
Authors: Zhu Zhen , Wang Weiwei , Cao Mingze , Liu Guanhui , Zhou Xuzheng , Li Bing , Shi Yuxiang , Zhang Jiyu 
Published: March 2021

Abstract
Background: The natural hosts of Shigella are conventionally humans and other primates; however, the host range of Shigella has been shown to expand to many animals. Although Shigella is becoming a huge threat to animals, there is limited information on the genetic background of local strains. The purpose of this study was to assess the presence of virulence factors and the molecular characteristics of S. flexneri isolated from calves with diarrhea.Methods: From 2014 to 2016, 54 S. flexneri isolates were collected from diarrhea, and their biochemical characteristics were determined according to API20E and virulence factors via PCR. The molecular characteristics of the isolates were studied by MLST, MLVA and PFGE.Results: Fifty-four S. flexneri isolates possessed four typical biochemical characteristics of Shigella. The prevalences of ipaH, virA, ipaBCD, ial, sen, set1A, and set1B were 100%, 100%, 77.78%, 79.63%, 48.15% and 48.15%, respectively. None of the studied strains possessed the stx gene. Regarding the differences in virulence factor distributions, the 54 S. flexneri isolates fell into seven gene profile types. Among these VTs, VT4 and VT6 were the most common, accounting for 74.07% of all VTs. MLVA based on 8 VNTR loci discriminated the isolates into 39 different MTs, PFGE based on NotI digestion divided the 54 isolates into 31 PTs, and MLST based on 15 housekeeping genes differentiated the isolates into 7 STs, with 1 ST (ST227) being novel.Conclusion: Our findings provide baseline information on the distribution of virulence genes in and the molecular characteristics of S. flexneri collected from diarrheal calves, which is a potential threat to public safety. These data will be important for addressing clinical and epidemiological issues regarding Shigellosis.
Imaging the communicating structures on oocyte through high-resolution spinning-disc microscope
Authors: Zhang Yan , Wang Ye , Zhang Hua 
Published: March 2021

Abstract
The communications between oocyte and granulosa cells are essential for ovarian folliculogenesis in mammals, and the communicating structures in the ZP had been widely reported. However, there was lack of direct approach to visualize these structures in a 3D model with a high-resolution. Here, we present a protocol to image the structures which derived from oocytes by combining endogenous fluorescent mouse models with a high-resolution spinning disc-confocal system in details
Bedside Diffuse Optical Tomography of Disrupted Brain Connectivity During Acute Stroke
Authors: Burke Broc , Bergonzi Karla , Sherafati Arefeh , Fishell Andrew , Burns-Yocum Tracy , Ferradal Silvina , Palanca Ben , Dhar Rajat , Kumar Gyanendra , Lee Jin-Moo , Culver Joseph , Eggebrecht Adam 
Published: March 2021

Abstract
Neurological instability during the first days following ischemic stroke reflects the potential to salvage brain tissue at risk for infarct growth, hemorrhagic transformation, or malignant edema. However, current clinical care provides intermittent assessments on the order of at least an hour apart potentially missing the early detection of neurological deterioration. Optical imaging offers a continuous, portable alternative to the current combination of physical exams and radiological imaging. However, optical imaging technology has heretofore lacked the crucial combination of portability, resolution, and coverage required for assessing distributed brain function. Here, we present a portable high-density diffuse optical tomography (HD-DOT) system that overcomes these limitations to assess neurological disruption at the bedside. We imaged patients within 72 hours after ischemic stroke onset and demonstrate that HD-DOT is sensitive to both the presence of stoke (Cohen’s d = 1.5, p = 1.2 × 10− 3) as well as the degree of neurological disruption measured by NIH Stroke Scale (R2 = 0.69, p = 4.7 × 10− 4).
Community-Clinical Partnership: Engaging health navigators to support refugees and non-refugee immigrants amidst the COVID-19 pandemic
Authors: Harris Megan A , Lupone Christina D , Asiago-Reddy Elizabeth , Anderson Kathryn B , Cronkright Peter , Blatt Steven D , Turbide Sophia , Kambhampaty Abby , Sous Waseem , Goodman Alexandra , Lichtenstein Daniel A , Mozo Nicole , Blatt Mia , Castricone Felicia , Shaw Andrea V 
Published: March 2021

Abstract
Background: The COVID-19 pandemic has exacerbated health inequities in vulnerable populations. Linguistic and sociocultural barriers, misinformation, and mistrust of Western medicine hinder public health outreach to diverse refugee and non-refugee immigrant communities. SUNY Upstate Medical University partnered with local resettlement agencies and health navigators to expand outreach and screening to this hard-to-reach population. Methods: Health navigators engaged participants for enrollment and screening. SARS-CoV2 status was assessed via RT-PCR of saliva swabs. Surveys captured COVID-19-related psychosocial behavioral insights.Results: Over 9 weekly sessions, 603 individuals in 195 refugee/immigrant households from 27 countries of origin were screened. COVID-19 positivity rate was 2% for households and 0.2% amongst individuals. Surveys provided insight into households’ concerns and health behaviors, and a space to reinforce protective behaviors and address misinformation and stigma. Conclusions: During these unprecedented times, our interdisciplinary community-clinical partnership successfully implemented COVID-19 screening, outreach, and support to local refugees and non-refugee immigrants through trusted networks of culturally, socially, linguistically congruent health navigators.
p16 Expression Represses DNA Damage Repair via a Novel Ubiquitin-Dependent Signaling Cascade.
Authors: Molkentine David , Molkentine Jessica M. , Bridges Kathleen A. , Sheth Aakash , Valdecanas David R , Bahri Rishi , Hefner Andrew J. , Kumar Manish , Yang Liang , Abdelhakiem Mohamed , Pifer Phillip M. , Sandulache Vlad C. , Beadle Beth M. , Thames Howard D. , Mason Kathryn A. , Pickering Curtis R. , Meyn Raymond E. , Skinner Heath D. 
Published: March 2021

Abstract
Human papillomavirus (HPV) drives the development of squamous cell carcinoma at several sites, including the oropharynx. Generally, the presence of HPV renders a tumor more sensitive to DNA-damaging therapies such as radiation; however, the mechanism behind this phenomenon is elusive. Previous studies have shown that p16, the clinically utilized surrogate for HPV tumor positivity, can render cells more sensitive to radiation. In the current manuscript, using a combination of immunoprecipitation mass spectrometry (IP/MS), in vivo and in vitro modulation and clinical tumor profiling, we identify a novel ubiquitin-dependent signaling pathway linking p16 to increased activity of the transcription factor SP1 leading to increased HUWE1 transcription and degradation of ubiquitin-specific protease 7 (USP7). This pathway is activated in HPV-positive tumor cells, leading to an absence of TRIP12, decreased DNA damage repair and increased mitotic death following radiation. As USP7 inhibitors are currently in clinical trials, this pathway provides a novel means by which radioresistant tumors may be targeted to increase response and improve outcome.
Person-centred practice and technology - a complex intervention study in two Norwegian nursing homes
Authors: Grøndahl Vigdis Abrahamsen , Anker-Hansen Camilla , Fagerli Liv Berit , Helgesen Ann Karin , Rummelhoff Guri , Halvorsrud Liv , Bååth Carina 
Published: March 2021

Abstract
Objective: The overall objective is to explore how the implementation of person-centred practice and use of technology affects patients’ and relatives’ perceptions of care quality and healthcare personnel’s job satisfaction, as well as shed light on nursing students’ experiences when performing person-centred interventions. In this paper, the interventions used to assure implementation of person-centredness and use of technology in relation to patients are described to facilitate an increased understanding of the study’s future results. Results: A person-centred practice framework was introduced and facilitated in three phases. These included: 1) Establishing a project group consisting of ward leaders and researchers. Workshops and meetings were held with the healthcare personnel to inform and discuss the concept of person-centred practice, use of technology and the interventions: “one-to-one-contact”, collection of life stories, advanced care planning, and point prevalence of wounds, including pressure ulcers, incontinence associated dermatitis and developing a skin care bundle. 2) A twelve months’ intervention period including facilitated innovation groups for personnel and leaders and conducting the four interventions. 3) Discussion of results with personnel. Inhibitors and promoters for the continuation of interventions will be highlighted. Strategies will ensure the further development of person-centred practice and use of technology in nursing homes.