bluuebunny/crossref_metadata_2025_split huggingface dataset:
FAM83A is a Potential Biomarker for Breast Cancer Initiation
Authors: Marino Natascia , German Rana , Podicheti Ram , Rockey Pam , Sandusky George E. , Temm Constance J. , Nakshatri Harikrishna , Addison Rebekah J. , Selman Bryce , Althouse Sandra K. , Storniolo Anna Maria V. 
Published: October 2021

Abstract
Background: Family with sequence similarity 83 member A (FAM83A) presents oncogenic properties in several cancers including breast cancer (BC). Recently, we reported FAM83A overexpression in normal breast tissues from women at high risk of breast cancer. We now hypothesize that FAM83A is a key factor in BC initiation. Methods: Immunohistochemical staining was used to evaluate FAM83A protein levels in both a normal breast tissue microarray (TMA, N=411) and a breast tumor TMA (N=349). EGFR staining and its correlation with FAM83A expression were also assessed. Lentivirus-mediated manipulation of FAM83A expression in primary and hTERT-immortalized breast epithelial cells was employed. Biological and molecular alterations upon FAM83A overexpression/downregulation and FAM83A’s interaction partners were investigated.Results: TMA analysis revealed a 1.5-fold increase in FAM83A expression level in BC cases as compared with normal breast tissues (p<0.0001). FAM83A protein expression was directly correlated with EGFR level in both normal and BC tissues. In in vitro assays, exogenous expression of FAM83A in either primary or immortalized breast epithelial cells promoted cell viability and proliferation. Additionally, Ingenuity Pathway Analysis (IPA) revealed that in normal cells FAM83A is involved in cellular morphology and metabolism. Mass spectrometry analysis identified DDX3X and LAMB3 as potential FAM83A interaction partners in primary cells, while we detected FAM83A interaction with cytoskeleton reorganization factors, including LIMA1, MYH10, PLEC, MYL6 in the immortalized cells.Conclusions: This study shows that FAM83A promotes metabolic activation in primary epithelial cells and survival in immortalized cells. These findings support its role in early breast oncogenesis.
Circulating Cell Free DNA and Citrullinated Histone H3 As Useful Biomarkers of NETosis In Endometrial Cancer.
Authors: Ronchetti Livia , Terrenato Irene , Corrado Giacomo , Goeman Frauke , Donzelli Sara , Mandoj Chiara , Merola Roberta , Zampa Ashanti , Carosi Mariantonia , Blandino Giovanni , Conti Laura , Lobascio Anna Maria , Iacobelli Marcello , Vizza Enrico , Piaggio Giulia , Gurtner Aymone 
Published: October 2021

Abstract
Background. Cancer mortality is mainly caused by organ failure and thrombotic events. It has been demonstrated that NETosis, a chromatin release mechanism implemented by neutrophils, may contribute to these lethal systemic effects. Our aim was to investigate NETosis biomarkers in endometrial cancer (EC).Methods. The experiments were conducted on 21 healthy subject (HS) with no gynecological conditions, and on 63 EC patients. To assess the presence of NETosis features IHC and IF was performed using antibodies against citrullinated histone H3 (citH3), neutrophil elastase (NE) and H2B. Serum levels of cell free DNA (cfDNA), cell free mitochondrial DNA (cfmtDNA) and citH3 were measured by qPCR using one microliter of deactivated serum, and by ELISA assay respectively. Fragmentation pattern of serum cfDNA was analyzed using the Agilent 2100 Bioanalyzer and High Sensitivity DNA Chips. Receiver operating characteristic (ROC) analysis was used to identify a cut off for cfDNA and cfmtDNA values able to discriminate between ECs and HSs. Correlation analysis and multiple correspondance analysis (MCA), between cfDNA, mitcfDNA, citH3 and blood parameters were used to identify the potential association among serum parameters in EC grades. Results. We demonstrated the presence of NETosis features in tissues from all EC grades. Serum cfDNA and cfmtDNA levels discriminate ECs from HSs and a direct correlation between citH3 and cfDNA content and an inverse correlation between cfmtDNA and citH3 in EC sera was observed, not detectable in HSs. MCA indicates cfDNA, cfmtDNA and citH3 as features associated to G1 and G2 grades. A correlation between increased levels of cfDNA, citH3 and inflammation features was found. Finally, serum nucleosomal cfDNA fragmentation pattern varies in EC sera and correlates with increased levels of cfDNA, citH3, lymphocytes and fibrinogen.Conclusion. Our data highlight the occurrence of NETosis in EC and indicate serum cfDNA and citH3 as noninvasive biomarkers of tumor-induced systemic effects in endometrial cancer.
One-Dimensional Bis(dipyrrinato)zinc(II)-Linked Porphyrinatozinc(II) Polymer: Synthesis, Exfoliation Into Single Wires, and Photofunctionality
Authors: Mustafar Suzaliza , Ogino Yasuyo , Nishihara Hiroshi 
Published: October 2021

Abstract
One-dimensional bis(dipyrrinato)zinc(II)-linked porphyrinatozinc(II) polymer, 2 were synthesized by facile metal complexation reaction between 5,15-bis(3,5-dioctyloxyphenyl)-10,20-bis(dipyrrinato)porphyrinatozinc(II),1 and zinc(II) acetate. The bulky substituents on the porphyrin units allows 2 to be exfoliated into single molecular wires with a 2.8 nm height and 1.4 Om length. 2 exhibited promising photofunctionality derived from electronic interaction between bis(dipyrrinato)zinc and porphyrinatozinc(II) moieties, which can be engaged in energy transfer system such as photonic molecular wires.
Characterization of the Different Subtypes of Immune Cells Infiltration to Aid Immunotherapy
Authors: Li Zhenqing , Mao Kai , Xue Qun 
Published: October 2021

Abstract
Backgroup：PD-1 ablation or PD-L1 specific monoclonal antibody (mAb) against PD-1 can recruit the accumulation of functional T cells, leading to tumor rejection in microenvironment and significantly improving the prognosis of various cancers. Despite these unprecedented clinical successes, intervention remission rates remain low after treatment, rarely exceeding 40%. The observation of PD-1/L1 blocking in patients is undoubtedly multifactorial, but the infiltrating degree of CD8+T cell may be an important factor for immunotherapeutic resistance.Method: We proposed two computational algorithms to reveal the immune cell infiltration (ICI) landscape of 1646 lung adenocarcinoma patients. Three ICI patterns were defined and the relative ICI scoring were depended on principal-component analysis.Result: A high ICI score was associated with the increased tumor mutation burden (TMB) and cell proliferation-related signaling pathways. Different cellular signaling pathways were observed in low ICI score subtypes, indicating cell proliferation active, and may be associated with poor prognosis. Conclusion: Our research identified that the ICI scores worked as an effective immunotherapy index, which may provide a promising therapeutic strategies on immune therapeutic for lung adenocarcinoma.
Technology Support Challenges and Recommendations for Adapting an Evidence-Based Exercise Program for Remote Delivery to Older Adults: Exploratory Mixed Methods Study
Authors: Gell Nancy , Hoffman Elise , Patel Kushang 
Published: October 2021

Background
Tele-exercise has emerged as a means for older adults to participate in group exercise during the COVID-19 pandemic. However, little is known about the technology support needs of older adults for accessing tele-exercise.

Objective
This study aims to examine the needs of older adults for transition to tele-exercise, identify barriers to and facilitators of tele-exercise uptake and continued participation, and describe technology support challenges and successes encountered by older adults starting tele-exercise.

Methods
We used an exploratory, sequential mixed methods study design. Participants were older adults with symptomatic knee osteoarthritis (N=44) who started participating in a remotely delivered program called Enhance Fitness. Before the start of the classes, a subsample of the participants (n=10) completed semistructured phone interviews about their technology support needs and the barriers to and facilitators for technology adoption. All of the participants completed the surveys including the Senior Technology Acceptance Model scale and a technology needs assessment. The study team recorded the technology challenges encountered and the attendance rates for 48 sessions delivered over 16 weeks.

Results
Four themes emerged from the interviews: participants desire features in a tele-exercise program that foster accountability, direct access to helpful people who can troubleshoot and provide guidance with technology is important, opportunities to participate in high-value activities motivate willingness to persevere through the technology concerns, and belief in the ability to learn new things supersedes technology-related frustration. Among the participants in the tele-exercise classes (mean age 74, SD 6.3 years; 38/44, 86% female; mean 2.5, SD 0.9 chronic conditions), 71% (31/44) had a computer with a webcam, but 41% (18/44) had little or no experience with videoconferencing. The initial technology orientation sessions lasted on average 19.3 (SD 10.3) minutes, and 24% (11/44) required a follow-up assistance call. During the first 2 weeks of tele-exercise, 47% of participants (21/44) required technical assistance, which decreased to 12% (5/44) during weeks 3 to 16. The median attendance was 100% for the first 6 sessions and 93% for the subsequent 42 sessions.

Conclusions
With appropriate support, older adults can successfully participate in tele-exercise. Recommendations include individualized technology orientation sessions, experiential learning, and availability of standby technical assistance, particularly during the first 2 weeks of classes. Continued development of best practices in this area may allow previously hard-to-reach populations of older adults to participate in health-enhancing, evidence-based exercise programs.
Using Venn Diagrams to Evaluate Digital Contact Tracing: Panel Survey Analysis
Authors: Daniore Paola , Nittas Vasileios , Moser André , Höglinger Marc , von Wyl Viktor 
Published: October 2021

Background
Mitigation of the spread of infection relies on targeted approaches aimed at preventing nonhousehold interactions. Contact tracing in the form of digital proximity tracing apps has been widely adopted in multiple countries due to its perceived added benefits of tracing speed and breadth in comparison to traditional manual contact tracing. Assessments of user responses to exposure notifications through a guided approach can provide insights into the effect of digital proximity tracing app use on managing the spread of SARS-CoV-2.

Objective
The aim of this study was to demonstrate the use of Venn diagrams to investigate the contributions of digital proximity tracing app exposure notifications and subsequent mitigative actions in curbing the spread of SARS-CoV-2 in Switzerland.

Methods
We assessed data from 4 survey waves (December 2020 to March 2021) from a nationwide panel study (COVID-19 Social Monitor) of Swiss residents who were (1) nonusers of the SwissCovid app, (2) users of the SwissCovid app, or (3) users of the SwissCovid app who received exposure notifications. A Venn diagram approach was applied to describe the overlap or nonoverlap of these subpopulations and to assess digital proximity tracing app use and its associated key performance indicators, including actions taken to prevent SARS-CoV-2 transmission.

Results
We included 12,525 assessments from 2403 participants, of whom 50.9% (1222/2403) reported not using the SwissCovid digital proximity tracing app, 49.1% (1181/2403) reported using the SwissCovid digital proximity tracing app and 2.5% (29/1181) of the digital proximity tracing app users reported having received an exposure notification. Most digital proximity tracing app users (75.9%, 22/29) revealed taking at least one recommended action after receiving an exposure notification, such as seeking SARS-CoV-2 testing (17/29, 58.6%) or calling a federal information hotline (7/29, 24.1%). An assessment of key indicators of mitigative actions through a Venn diagram approach reveals that 30% of digital proximity tracing app users (95% CI 11.9%-54.3%) also tested positive for SARS-CoV-2 after having received exposure notifications, which is more than 3 times that of digital proximity tracing app users who did not receive exposure notifications (8%, 95% CI 5%-11.9%).

Conclusions
Responses in the form of mitigative actions taken by 3 out of 4 individuals who received exposure notifications reveal a possible contribution of digital proximity tracing apps in mitigating the spread of SARS-CoV-2. The application of a Venn diagram approach demonstrates its value as a foundation for researchers and health authorities to assess population-level digital proximity tracing app effectiveness by providing an intuitive approach for calculating key performance indicators.
L’écriture et la vie selon Anne-Lise Grobéty
Authors: Cossy Valérie 
Published: October 2021

Dix ans après la disparition de l’écrivaine, cet article s’efforce de cerner ce qui rend mémorable l’écriture d’Anne-Lise Grobéty. Sans prétendre à l’analyse rigoureuse, il invite à saisir les ressources de l’union qu’elle a tissée de sa plume entre la légèreté et la gravité.
Keywords : Anne-Lise Grobéty, écriture, langue, humour, sens, auteur, lecteur, Henri Roorda.
Sovrapposizioni. Testo, immagine e fotografia ne "Il Giuoco dell’Oca" di Edoardo Sanguineti
Authors: Hufnagel Henning 
Published: October 2021

Il saggio legge il romanzo di Sanguineti Il Giuoco dell’Oca attraverso un nuovo focus: la medialità. Vi si dimostra come, anche senza includere alcuna immagine materiale, esso funzioni come fototesto, anzi costituisca un fototesto di livello potenziato: articolandosi in una serie ecfrastica, mettendo in scena tensioni riflessive tra lingua e immagine, simulando persino il modo di percezione dell’obiettivo fotografico. Attraverso le immagini, Sanguineti contesta non solo i modi narrativi convenzionali, ma spinge la sua sperimentazione anche oltre le avanguardie francesi con le quali dialoga, da Breton ai contemporanei Robbe-Grillet e Sollers.
Keywords: narratività, intermedialità, fotografia, Nouveau roman, Tel Quel.
"Harri eta herri/Piedra y pueblo" de Gabriel Aresti. Un abecedario vital
Authors: Kortazar Jon 
Published: October 2021

Tras la aparición de Harri eta Herri/Piedra y pueblo nada fue igual en la historia de la poesía vasca. Gabriel Aresti abrió un camino poético eficaz para la expresión de una sociedad que boqueaba bajo el franquismo con un riesgo serio de pérdida de la lengua, con una situación social sangrante y con abismales diferencias sociales, con la presencia en el ámbito internacional de nuevas formas de hacer política, entre las que destacó simbólicamente la Revolución Cubana, y que ya anunciaba la presencia de ETA. Por sus referencias culturales, que reunían a todo el arco creativo en el País Vasco, por su interés en retomar la tradición para darle nuevo aliento, por su concepto de poesía fácil e inteligible, por su compromiso social, Gabriel Aresti representó a través de su poesía una sociedad que sufría y luchaba, y sobre todo que vivía con dignidad.
Keywords: Aresti, Harri eta Herri, País Vasco, poesía vasca.
La ética como estética del presente (De la formación ideológica de Miguel Hernández)
Authors: Urrutia Jorge 
Published: October 2021

El artículo desea mostrar la evolución estética de Miguel Hernández desde la figura del poeta-pastor de sus inicios hasta el poeta neopopular del Cancionero y Romancero de ausencias, a través de su trayectoria ideológica. Se resalta la importancia de un poema habitualmente considerado menor, para su autodescubrimiento como poeta proletario y la fijación de una poética ética.
Keywords: Miguel Hernández, neopopularismo, poesía proletaria, poética.
Iconicidad y autorreflexión implícita en la poesía de Miguel Hernández
Authors: López Guil Itzíar 
Published: October 2021

Este trabajo se propone demostrar la existencia en la poesía de Miguel Hernández de diversos procedimientos autorreferenciales de carácter icónico con los que crea en sus poemas un segundo nivel de sentido de carácter implícito y metaliterario. Se trata de estrategias que conoce y descubre al lado de Sijé, cuando forma parte de El Gallo Crisis, y que, incluso tras trabar amistad con Neruda y cambiar de poética, seguirá explorando y poniendo en práctica hasta el prematuro e injusto final de su vida.
Keywords: Autorreferencialidad, iconicidad, Miguel Hernández, «Un carnívoro cuchillo», «Umbrío por la pena», «Como el toro he nacido para el luto», «Para la libertad», «Llegó con tres heridas».
Autobiografía, autorretrato y autorreferencia en el "Cancionero" de Miguel Hernández
Authors: Rovira José Carlos 
Published: October 2021

Desde los conceptos de autobiografía, autorretrato y autorreferencia se realiza la lectura de algunos poemas del Cancionero y romancero de ausencias, no tanto como una perspectiva surgida desde el campo de la teoría literaria, sino insistiendo en los valores que, en la última obra de Miguel Hernández, se entremezclan como dimensión de una existencia en la que sobrevivir era construir una escritura que daba cuenta de sí mismo. Reflejaba así su imagen e incidía metafóricamente en lo que estaban viviendo, como reflejo social y colectivo, aquellos que sufrían las mismas consecuencias de la represión, las cárceles y las condenas a muerte. La realidad provocaba la afirmación de sí mismo a través de recursos que, junto a retóricos, podían testimoniar una nueva dimensión de una poesía y una historia que podemos considerar como la de la derrota en la España de la postguerra civil.
Keywords: Autobiografía, autorretrato, autorreferencia, historia, sociedad.
Penilaian Risiko Kualitatif Masuknya Rabies Melalui Pergerakan Anjing dari Provinsi Jawa Barat ke Kota Surakarta
Authors: Permatasari Indri , Sumiarto Bambang , Susetya Heru 
Published: October 2021

Abstrak Lalu lintas anjing dari daerah tertular rabies ke daerah bebas rabies masih terjadi di Indonesia. Hal ini menjadi salah satu pemicu terhadap munculnya kasus rabies di daerah bebas. Penelitian ini bertujuan untuk melakukan penilaian risiko kualitatif kemungkinan masuknya rabies melalui pergerakan anjing konsumsi dari Jawa Barat ke Kota Surakarta, Provinsi Jawa Tengah. Metode pengumpulan data yang digunakan dalam penelitian ini menggunakan data primer dan data sekunder. Data primer diperoleh dari wawancara, kuesioner, pendapat pakar, dan observasi langsung di lapangan. Data sekunder diperoleh melalui kajian literatur, penelusuran publikasi ilmiah, dan dokumen dari instansi berwenang yang tidak dipublikasikan. Hasil penelitian menunjukkan bahwa penilaian pelepasan dari daerah asal anjing adalah “sedang” dengan ketidakpastian rendah. Tingkat kejadian rabies pada hewan di Provinsi Jawa Barat tahun 2019 sebesar 3,1%. Penilaian pendedahan adalah “tinggi” dengan ketidakpastian rendah karena frekuensi pengiriman anjing konsumsi dari Jawa Barat dilakukan setiap hari. Penilaian dampak adalah “tinggi” dengan ketidakpastian rendah karena ada dampak tunggal yang masuk dalam kategori signifikan di tingkat nasional. Hasil penelitian dapat disimpulkan bahwa penilaian risiko kualitatif masuknya rabies ke Kota Surakarta adalah tinggi dengan ketidakpastian rendah. Evaluasi kemungkinan kualitatif dapat mempertimbangkan tingkat kejadian rabies daerah asal dan frekuensi pengiriman anjing konsumsi setiap hari yang merupakan masalah penting risiko masuknya rabies.
Efficacy of Albendazole Against Strongylus sp. and Hematology Changes on Equine in Yogyakarta Special Region
Authors: Yuriadi Yuriadi , Tjahajati Ida , Mulyani Guntari Titik , Muqit Kelviano , Rahmanita Aidah 
Published: October 2021

The infestation of Strongylus sp. in horses can cause losses to horse breeders, including anorexia, anemia, gastrointestinal diseases and can cause death and decrease the horse population in DIY. Albendazole was a Benzimidazole preparation that is often used to treat worms in ruminants. This study also aims to determine the effect of Albendazole on blood images before and after treatment. The material used in this study were 10 horses with male and female sex, over 3 years old, and infected with Strongylus sp. with an infestation rate of 200 EPG in faeces. Before treatment of drug was carried out, the faeces was examined with Mc Master method. After treatment with Albendazole, the worm eggs were examined three times at intervals of three days. Routine examination of worm eggs and blood was carried out at the Laboratory of the Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada. The results showed that the EPG number decreased from the 0th, 3rd, 6th and 9th day of examinations. The average number of EPG on the 0th day was 990 eggs/gram, and the examination on the 9th day showed the number of eggs was 0 eggs/gram. The results of the hematology examination also showed no significant difference except for the high number of eosinophils on the 12th day after drug administration. The conclusion from this research is that Albendazole as a worm medicine can kill Srongylus sp. however, it does not affect the horse's hematology and health status.
Association Between the Angiotensin II/Angiotensin (1-7) Imbalance and Left Ventricular Hypertrophy in Patients with Heart Failure
Authors: Nikolov A. , Tzekova M. , Kostov K. , Kostadinovska A. , Blazheva S. 
Published: October 2021

Abstract

Introduction: Angiotensin II (AngII) and angiotensin-(1-7) [Ang-(1-7)] are key components of the renin angiotensin system (RAS). They exhibit counter-regulatory effects in the systemic circulation, as well as in tissues important for cardiovascular regulation.

Aim: To investigate the association between the AngII/Ang-(1-7) balance and left ventricular hypertrophy (LVH) in patients with heart failure and mid-range ejection fraction (HFmrEF).

Material and methods: 56 patients with HFmrEF were included, with a mean age of 65.62 ± 9.69 years and 22 age- and sex-matched healthy subjects, their mean age - 56.4 ± 5.53 years. The patients were divided in two subgroups: subjects with left ventricular hypertrophy (n = 32); (HFmrEF+LVH) and subjects without left ventricular hypertrophy (n = 24); (HFmrEFLVH). AngII and Ang-(1-7) levels were measured with an ELISA kit.

Results: Patients with HFmrEF+LVH showed significantly higher levels of AngII: 8.53 pg/mL (1.47 ÷ 13.0) than HFmrEF-LVH – 1.33 pg/mL (0.47 ÷ 6.93) and healthy controls – 1.53 pg/mL (0.27 ÷ 5.21); (KW = 3.48; p = 0.04). There was no significant difference between Ang-(1-7) levels in all patients compared to controls (p > 0.05). AngII/Ang-(1-7) ratio was significantly higher in all patients than controls: 3.81 (2.03 ÷ 6.66) vs. 1.5 (0.93 ÷ 2.06) (KW = 18.68; p < 0.001). Patients with HFmrEF+LVH showed statistically higher AngII/Ang-(1-7) ratio compared with controls (4.7 vs. 1.5). Moreover, subjects with LVH showed the highest AngII/Ang-(1-7) ratio among all particpants in the study. The AngII/Ang-(1-7) ratio correlated with LVH (r = -0.39; p = 0.03), DBP (r = 0.25; p = 0.04), HDL (r = 0.33; p = 0.01), SBP (r = 0.34; p = 0.01).

Conclusion: Our study showed an association between AngII/Ang-(1-7) ratio, blood pressure and LVH. The imbalance between Ang-II and Ang-(1-7) could contribute to the mechanisms determining LVH in HFmrEF. Further studies are warranted to clarify whether evaluation of serum Ang-II/Ang-(1-7) ratio could predict LVH development in patients with HFmrEF.