bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Implications of Being “In it Together”: Relationship Satisfaction and Joint Health Behaviors Predict Better Health and Stronger Concordance Between Partners
Authors: Wilson Stephanie J , Novak Joshua R 
Published: December 2021

Abstract

Background
Extensive evidence shows that satisfying marriages boost physical health and longevity. A separate literature reveals strong concordance in couples’ health, but the relationship processes that contribute to health concordance remain poorly understood.

Purpose
The current study examined whether relationship satisfaction and joint health behaviors—the extent to which couples eat, sleep, and exercise together—are associated simultaneously with better health and greater health similarity between partners.

Methods
Heterogeneous variance multilevel models were applied to data from 234 married couples (Mage = 46, Range = 20–84) reporting on their relationship satisfaction, joint health behaviors, and four health indicators—health satisfaction, depressive symptoms, comorbidities, and medication use.

Results
More satisfied couples engaged in more joint health behaviors than less satisfied counterparts. When joint health behaviors and relationship satisfaction were examined as separate fixed effects, both predicted greater health satisfaction and fewer depressive symptoms. More joint health behaviors were also associated with less medication use. When both were modeled together, only relationship satisfaction predicted depressive symptoms. By contrast, in random effects, joint health behaviors predicted greater similarity in health satisfaction, depressive symptoms, and comorbidities. Relationship satisfaction only predicted more similar depressive symptoms.

Conclusions
Although more satisfied couples engaged in more joint health behaviors. relationship satisfaction and joint health behaviors uniquely predicted couples’ health quality and concordance, suggesting that distinct mechanisms may drive better health and stronger health resemblance.
An integrated approach to evaluate different tetracycline derivatives for formulary decisions
Authors: Zidaru Andrei , Phe Kady , Lasco Todd M , Tam Vincent H 
Published: December 2021

AbstractPurposeStenotrophomonas maltophilia has emerged as a critical opportunistic pathogen associated with significant morbidity and mortality. Tetracycline derivatives have been recognized as alternative treatment options, but they have varied pharmacokinetic properties. An integrated approach to different tetracycline derivatives for formulary decisions is reported.MethodsThe minimum inhibitory concentration (MIC) data from clonally diverse bloodstream S. maltophilia isolates were examined, along with the pharmacokinetic profiles of 4 tetracycline derivatives, to predict achievable pharmacodynamic exposures with standard intravenous dosing regimens. Antimicrobial therapy was assessed using the ratio of daily drug acquisition cost relative to the ratio of the free-drug area under the time-concentration curve (fAUC) to minimum inhibitory concentration (MIC) for 90% of isolates (fAUC/MIC90).ResultsIn our analysis, minocycline had the greatest fAUC/MIC90. Doxycycline was the most financially preferred agent, as calculated using 2020 average wholesale price for base-case estimates of drug acquisition cost.ConclusionAn integrated evaluation for antimicrobial formulary decision-making addressed local susceptibility data, pharmacokinetics, pharmacodynamics, dosing regimens, and drug acquisition costs. This comprehensive method is more objective than the conventional approach and warrants validation.
The relationship of vancomycin 24-hour AUC and trough concentration
Authors: Nix David E , Davis Lisa E , Matthias Kathryn R 
Published: December 2021

Abstract

Purpose
Prior to the 2020 release of a joint consensus guideline on monitoring of vancomycin therapy for serious methicillin-resistant Staphylococcus aureus (MRSA) infections, clinicians had escalated vancomycin doses for 2 decades while targeting trough concentrations of 15 to 20 µg/mL, leading to an increased frequency of nephrotoxicity. For MRSA infections, the 2020 guideline recommends adjusting doses to achieve a 24-hour area under the concentration-time curve (AUC) of 400 to 600 µg · h/mL; however, monitoring of trough concentrations has been entrenched for 3 decades. Calculating dose regimens based on AUC will require obtaining an increased number of vancomycin serum concentrations and, possibly, advanced software. The aim of this investigation was to determine the relationship between AUC and trough concentration and the influence of dosing regimen on goal achievement.

Methods
The relationship between trough concentration and AUC was explored through derivation of an equation based on a 1-compartment model and simulations.

Results
24-hour AUC is related to dosing interval divided by half-life in a nonlinear fashion. The target trough concentration can be individualized to achieve a desired AUC range, and limiting use of large doses (&gt;15-20 mg/kg) can protect against excessive 24-hour AUC with trough-only monitoring.

Conclusion
After initially determining pharmacokinetic parameters, subsequent monitoring of AUC can be accomplished using trough concentrations only. Trough concentration may be used as a surrogate for AUC, although the acceptable target trough concentration will vary depending on dosing interval and elimination rate constant. This work included development of an AUC-trough equation to establish a patient-specific target for steady-state trough concentration.
Amyloid β protein negatively regulates human platelet activation induced by thrombin receptor-activating protein
Authors: Mizutani Daisuke , Tokuda Haruhiko , Onuma Takashi , Uematsu Kodai , Nakashima Daiki , Ueda Kyohei , Doi Tomoaki , Enomoto Yukiko , Matsushima-Nishiwaki Rie , Ogura Shinji , Iida Hiroki , Kozawa Osamu , Iwama Toru 
Published: December 2021

ABSTRACT
Amyloid β protein deposition in cerebral vessels, a characteristic of Alzheimer's disease, is a risk factor for intracerebral hemorrhage. Amyloid β protein directly modulates human platelet function; however, the exact mechanism of action is unclear. Therefore, we investigated the effects of amyloid β protein on human platelet activation using an aggregometer with laser scattering. Amyloid β protein decreased platelet aggregation induced by thrombin receptor-activating protein, but not by collagen and ADP. Amyloid β protein also suppressed platelet aggregation induced by SCP0237 and A3227. Platelet-derived growth factor-AB secretion and phosphorylated-heat shock protein 27 release by thrombin receptor-activating protein were inhibited by amyloid β protein. Additionally, thrombin receptor-activating protein-induced phosphorylation of JNK and p38 MAP kinase was reduced by amyloid β protein. Collectively, our results strongly suggest that amyloid β protein negatively regulates protease-activated receptor-elicited human platelet activation. These findings may indicate a cause of intracerebral hemorrhage due to amyloid β protein.
Overexpression of SRO_3163, a homolog of Streptomyces antibiotic regulatory protein, induces the production of novel cyclohexene-containing enamide in Streptomyces rochei
Authors: Misaki Yuya , Nindita Yosi , Fujita Kota , Fauzi Amirudin Akhmad , Arakawa Kenji 
Published: December 2021

ABSTRACT
Streptomyces antibiotic regulatory proteins (SARPs) are well characterized as transcriptional activators for secondary metabolites in Streptomyces species. Streptomyces rochei 7434AN4 harbors 15 SARP genes, among which 3 were located on a giant linear plasmid pSLA2-L and others were on the chromosome. Some SARP genes were cloned into an integrative thiostrepton-inducible vector pIJ8600, and their recombinants were cultivated. The recombinant of SARP gene, SRO_3163, accumulated a UV-active compound YM3163-A, which was not detected in the parent strain and other SARP recombinants. Its molecular formula was established to be C8H11NO. Extensive NMR analysis revealed that YM3163-A is a novel enamide, 2-(cyclohex-2-en-1-ylidene)acetamide, and its structure was confirmed by chemical synthesis including Horner–Wadsworth–Emmons reaction and ammonolysis.
wTAM: a web server for annotation of weighted human microRNAs
Authors: Cui Chunmei , Fan Rui , Zhou Yuan , Cui Qinghua 
Published: December 2021

Abstract It is well-known that some microRNAs (miRNAs) are more important than the others for life, hinting the wide range of miRNA in essentiality or importance. Functional enrichment analysis is a quite pervasive method to dig out the underlying biological pathway for a given gene list and several tools of miRNA set enrichment analysis have been developed. However, all those tools treat each miRNA equally and neglect the importance score of miRNA itself, which could be an obstacle to seek more insightful biological processes for researchers. Here, we developed wTAM, a tool for annotation of weighted human miRNAs, introducing the miRNA importance scores into enrichment analysis. In addition, the annotation repository has been enlarged comparing to TAM. Finally, the case study demonstrated the availability and flexibility of wTAM.Availability and implementationwTAM is freely available at http://www.cuilab.cn/wtam/.Supplementary informationSupplementary data are available at Bioinformatics Advances online.
miRe2e: a full end-to-end deep model based on transformers for prediction of pre-miRNAs
Authors: Raad Jonathan , Bugnon Leandro A , Milone Diego H , Stegmayer Georgina 
Published: December 2021

Abstract

Motivation
MicroRNAs (miRNAs) are small RNA sequences with key roles in the regulation of gene expression at post-transcriptional level in different species. Accurate prediction of novel miRNAs is needed due to their importance in many biological processes and their associations with complicated diseases in humans. Many machine learning approaches were proposed in the last decade for this purpose, but requiring handcrafted features extraction to identify possible de novo miRNAs. More recently, the emergence of deep learning (DL) has allowed the automatic feature extraction, learning relevant representations by themselves. However, the state-of-art deep models require complex pre-processing of the input sequences and prediction of their secondary structure to reach an acceptable performance.

Results
In this work, we present miRe2e, the first full end-to-end DL model for pre-miRNA prediction. This model is based on Transformers, a neural architecture that uses attention mechanisms to infer global dependencies between inputs and outputs. It is capable of receiving the raw genome-wide data as input, without any pre-processing nor feature engineering. After a training stage with known pre-miRNAs, hairpin and non-harpin sequences, it can identify all the pre-miRNA sequences within a genome. The model has been validated through several experimental setups using the human genome, and it was compared with state-of-the-art algorithms obtaining 10 times better performance.

Availability and implementation
Webdemo available at https://sinc.unl.edu.ar/web-demo/miRe2e/ and source code available for download at https://github.com/sinc-lab/miRe2e.

Supplementary information
Supplementary data are available at Bioinformatics online.
SCRIP: an accurate simulator for single-cell RNA sequencing data
Authors: Qin Fei , Luo Xizhi , Xiao Feifei , Cai Guoshuai 
Published: December 2021

AbstractMotivationRecent advancements in single-cell RNA sequencing (scRNA-seq) have enabled time-efficient transcriptome profiling in individual cells. To optimize sequencing protocols and develop reliable analysis methods for various application scenarios, solid simulation methods for scRNA-seq data are required. However, due to the noisy nature of scRNA-seq data, currently available simulation methods cannot sufficiently capture and simulate important properties of real data, especially the biological variation. In this study, we developed scRNA-seq information producer (SCRIP), a novel simulator for scRNA-seq that is accurate and enables simulation of bursting kinetics.ResultsCompared to existing simulators, SCRIP showed a significantly higher accuracy of stimulating key data features, including mean–variance dependency in all experiments. SCRIP also outperformed other methods in recovering cell–cell distances. The application of SCRIP in evaluating differential expression analysis methods showed that edgeR outperformed other examined methods in differential expression analyses, and ZINB-WaVE improved the AUC at high dropout rates. Collectively, this study provides the research community with a rigorous tool for scRNA-seq data simulation.Availability and implementationhttps://CRAN.R-project.org/package=SCRIP.Supplementary informationSupplementary data are available at Bioinformatics online.
P012 RISK FACTORS FOR INCISIONAL HERNIA AND ITS RECURRENCE: OBSERVATIONAL, AMBISPECTIVE STUDY
Authors: Jimenez Lia , Rojas Alexei , Merchan Angela , Velasquez Braulio , Fernandez Daniel , Bravo Andres 
Published: December 2021

Abstract

Aims
In patients with herniorrhaphy treated in a 3rd level hospital in the Southwestern of Colombia from January 2014 to March 2020, determine the frequency of incisional hernia recurrence and the risk factors related to.

Materials and methods
Observational, ambispective study that included patients older than 15 years with a history of incisional hernia that agreed to participate and signed a consent form. Patients with incomplete data or who underwent surgery in another institution were excluded. Follow-up appointments every 3 months were made to evaluate the incidence of hernia recurrence.

Results
112 patients were included, 64.3% female with a mean age of 58.6-year-old. The frequency of recurrence was 38.4% with a mean of appearance of 22.9 months; 44.2% were repaired with only one technique and 39.5% with non-mesh. Non-use of mesh increased the risk for recurrence (RR 2.02; CI95%: 1.17-3.48). Other risk factors were urgent surgery (RR 1.82; CI95%: 1.14-2.91), defect closure with multifilament suture (RR 1.61; CI95%: 1.15-2.25), not do adhesiolysis (RR 3.17; CI 95%; 0.85 – 11.76) and the no use of postoperative antibiotics (RR 1.67M CI95%: 0.97-2.89).

Conclusions
Incisional hernia recurrences increase with time. Therefore, a follow-up of at least for 3 years should be guaranteed to avoid undiagnosed cases. Risk factors identified like absorbable multifilament sutures and non-use of the mesh must be removed from the surgery plans. Furthermore, a specialized in-hospital group of the abdominal wall and an institutional protocol would help to diminish this complication.
P015 ABDOMINAL WALL PREPARATION PRECEDING COMPLEX ABDOMINAL WALL RECONSTRUCTION (CAWR) - SINGLE CENTER INITIAL EXPERIENCES UTILIZING BOTULINUM TOXIN A (BTA) ACCOMPANIED IN SELECTED PATIENTS BY PREOPERATIVE PROGRESSIVE PNEUMOPERITONEUM (PPP)
Authors: Husu Henrik , Valle Sanna , Scheinin Tom , Vironen Jaana 
Published: December 2021

Abstract

Aim
Complex ventral hernia (CVH) treatment due to large abdominal wall defects or loss of domain is challenging. BTA-injection in lateral abdominal wall causing flaccid paralysis and elongation, so called chemical component separation (CCS), might facilitate closure of large fascial defects. Combining preoperative progressive pneumoperitoneum might help restore abdominal content in severe loss of domain situations. We studied the results after CAWR following abdominal wall preparation with BTA and possible PPP. Primary aim was to report fascial defect closure rate without need for surgical component separation.

Material and Methods
All electively operated patients to date that were treated preoperatively with BTA, including all patients treated with PPP.

Results
Hospital index patient received preoperative BTA in January 2018. Since then, altogether 42 patients underwent CCS prior to CAWR. Average patient suffered from obesity (mean BMI 31), 30% had diabetes, and a third were active smokers. Mean hernia defect area exceeded 200 cm2. All operations were mesh repairs. Surgical approach was mostly (88%) open retromuscular. Abdominal wall reconstruction via fascial closure was achieved in 93% of cases with only 21% needing surgical component separation. Preoperative morbidity was common in patients undergoing PPP. Around 40% of all patients had postoperative complications, half of which were surgical complications. One patient died of a yet unknown cause on the third postoperative day. There were no recurrences within median 15 months follow-up.

Conclusions
Restoring abdominal wall continuity without frequent need for surgical component separation seems likely in CVH following CCS.
P022 OUTCOMES OF ABDOMINAL WALL REPAIR ASSOCIATED WITH INTESTINAL TRANSIT RECONSTRUCTION SURGERY
Authors: Munoz-Rodriguez Joaquin , Monclús Javier López , Lersundi Alvaro Robin Valle de , Hernando Luis Blázquez , Ureña Miguel Ángel García 
Published: December 2021

Abstract

Aim
Analyze and evaluate the results obtained in patients undergoing transit reconstruction surgery in which an abdominal wall reconstruction (AWR) is associated using a multidisciplinary approach.

Material and Methods
All patients who underwent an intestinal transit reconstruction associated with an AWR surgery were identified from a prospectively maintained multicenter database.
Short and long-term results have been analyzed, especially AWR outcomes.

Results
10 patients were identified. 60% were men. Mean time since previous surgery was 1.66 years.
8 cases (80%) associated a midline incisional hernia with the parastomal hernia.
3 (30%) bilateral posterior component separation (PCS) Madrid transverse abdominis muscle release (Madrid TAR) modification, 5 (50%) unilateral Madrid TAR, 1 (10%) PCS Carbonell, and 1 (10%) Rives-Stoppa techniques were performed.
A double mesh reconstruction technique was used in 60% of the patients, associating absorbable mesh with a permanent mesh.
One patient presented a paucisymptomatic colorectal anastomosis fistula, that could be managed conservatively. A case of postsurgical ileus was also evidenced.
Surgical site ocurrences (SSO) were recorded in 4 patients (40%), all of them related to surgical site infection that required a bedside wound opening.
During a mean follow-up of 24 (+/- 15) months, there was no evidence of hernia recurrence. No cases of bulging, chronic mesh infection or chronic pain were reported.
No case of mortality was recorded in the series.

Conclusions
Intestinal transit reconstruction surgery associated with an AWR, with a multidisciplinary team managed, presents acceptable long-term results, despite the high SSO associated.
P028 COMBINING SEVEN ITEMS OF THE TOOLBOX TO TREAT A LARGE INCISIONAL HERNIA WITH HETEROTOPIC OSSIFICATION: A CASE REPORT
Authors: Malaibari Zaid , Niebuhr Henning , Dag Halil 
Published: December 2021

Abstract

Aim
We present our approach of treating a W3 (EHS-Classification) incisional hernia with heterotopic ossification in the abdominal wall.

Material and Methods
a 62-years-old female patient presented with a hernia in her inverted-T incision (midline and transverse) after undergoing multiple laparotomies. The CT-scan showed calcified structures within the abdominal wall. We planned the extensive reconstruction after preoperative Botox injections.

Results
The 20x25 cm hernial sack contained parts of the stomach and colon. The dissection of the midline and transverse scars was challenging with the needed removal of scattered pieces of heterotopic bone tissues. After dissecting the retro-muscular space, the fascial edges were 25 cm apart. With bilateral transversus abdominis release (TAR), It was reduced to 20 cm. The posterior fascia was approximated, leaving a central 12 cm defect, and a smaller lateral defect, which we covered using open-IPOM and underlay techniques respectively. A 30x40 cm mesh in sublay position was placed and fascial traction was applied on the anterior fascia. With the resulting defect of 16 cm, a tension-free closure was still not possible, and we bridged the gap with a mesh in inlay position.

Conclusions
Despite combining pre-operative Botox injection and fascial traction with TAR, complete closure of the fascia was not possible. IPOM, sublay, underlay and inlay bridging were needed. Specialized hernia surgeons should be familiar with a wide range of different techniques to deal with such cases.
P029 HOW DOES THE MADRID APPROACH WORK
Authors: Blázquez Luis Alberto , Oto Diego , Porrero Belén , Molina José Manuel , Pastor Paula , Nieto María del Rocío 
Published: December 2021

Abstract

Aim
The Madrid APPROACH is the combination of an absorbable mesh and a permanent retromuscular mesh for the treatment of the complex abdominal wall problems. It has been controversial because of the need of two different meshes. We present a clinic case to show the utility of this technique and how it allows rebuilding the inguinal ligament.

Material and Methods
78 years old woman who underwent a right ilioinguinal and obturatriz lymphadenectomy due to a melanoma. Incisional hernia fixed in 2018 with a retromuscular polyester mesh. New incisional iliac hernia (L3) over the right iliac vessels, with an absence of inguinal ligament, right rectus atrophy, and the previous mesh being part of the sac.
Surgery: incision over the previous scar. Wide dissection of the preperitoneal space, Retzius space and lateral to the cuadratus lumborum, retrodiafragmatic dissection, lateral transverse abdominus release, and cross-over to the retrorectal left space. Preperitoneal BioA mesh and an upper 40x40cm medium weight polipropilene mesh set to both Cooper ligaments.

Results
After two and a half months, a PET-TC showed the BioA mesh perfectly adapted to the abdominal wall and rebuilt a new inguinal ligament. Also intense FDG capitation of the mesh due to the high cellular metabolism.
Two years later the patient has a continent abdominal wall, the follow up TC shows the disappearance of the absorbable mesh and the perfect abdominal wall rebuilt.

Conclusions
The BioA mesh acts like a tissue scaffold for new conjunctive tissue as we see the intense FDG captation. The Madrid APPROACH allows giving response to very complex abdominal wall problems.
P040 INFLAMMATORY REACTION TO ALD COATED POLYPROPYLENE MESHES USED FOR HERNIA REPIAR. AN EXPERIMENTAL STUDY IN ANIMALS
Authors: Khamidov Magomed , Razin Ragimov , Maksumova Abay , Abdullaeva Naida , Gasanbekova Zeinab , Abdulagatov Aziz 
Published: December 2021

Abstract

Aim
The main goal of the present work is to study and ability of Atomic Layer Depostion (ALD) ultra-thin (&lt;100nm) ceramic films on polypropylene (PP) hernia meshes to prevent the mesh-associated infections (post-surgery complications).

Material and Methods
Three types of ALD nanofilms were examined: Al2O3, TiO2, or TixVyOz. 10 rats and 5 rabbits were used to evaluate the tissue reaction of surface modified PP meshes and for biomechanical (antibacterial, inflammation effect), biocompatibility, and barrier testing of the healed tissue. The ALD coated PP meshes were implanted into rabbits and rats together with uncoated ones. After 10, 20, 30 and 60 days, the hernia meshes with the surrounding soft tissues were removed and fixed for histological and cytological studies.

Results
The TixVyOz (28nm) film showed enhanced antibacterial activity compare to Al2O3 and TiO2 films. The histology was performed on coated and uncoated PP mesh samples.

Conclusions
The ALD TixVyOz film helped to avoids formation of rough scar tissue (microscale roughness, which prevents ability biofilm formation) and, reduction of eosinophilic-cell and lymphocytic-cell reactions of the tissues surrounding the mesh, illustrate good integration into the surrounding tissue with minimal inflammatory reaction and minimal adhesions to intra-abdominal structures. The ALD film was highly effective in inhibiting S. aureus and E. coli bacteria adhesion and exhibited excellent biological activity in promoting osteoblastic adhesion. Speculatively, presence of vanadyl (≡V=O:) chemical groups, either on the surface or in the bulk, believe to play a key role in high performance of the TixVyOz compare to TiO2.