bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pvr and downstream signaling factors are required for spreading of Drosophila hemocytes at larval wound sites
Authors: Tsai Chang-Ru , Jacobson Alec , Sankoorikkal Niki , Chirinos Josue D. , Burra Sirisha , Wang Yan , Makthal Nishanth , Kumaraswami Muthiah , Galko Michael J. 
Published: June 2021

AbstractTissue injury is typically accompanied by inflammation. In Drosophila melanogaster, wound-induced inflammation involves adhesive capture of hemocytes at the wound surface followed by hemocyte spreading to assume a flat, lamellar morphology. The factors that mediate this cell spreading at the wound site are not known. Here, we discover a role for the Platelet-derived growth factor (PDGF)/ Vascular endothelial growth factor (VEGF)-related receptor (Pvr) and its ligand, Pvf1, in blood cell spreading at the wound site. Pvr and Pvf1 are required for spreading in vivo and in an in vitro spreading assay where spreading can be directly induced by Pvf1 application or by constitutive Pvr activation. In an effort to identify factors that act downstream of Pvr, we performed a genetic screen in which select candidates were tested to determine if they could suppress the lethality of Pvr overexpression in the larval epidermis. Some of the suppressors identified are required for epidermal wound closure, another Pvr-mediated wound response, some are required for hemocyte spreading in vitro, and some are required for both. One of the downstream factors, Mask, is also required for efficient wound-induced hemocyte spreading in vivo. Our data reveals that Pvr signaling is required for wound responses in hemocytes (cell spreading) and defines distinct downstream signaling factors that are required for either epidermal wound closure or hemocyte spreading.
Genetic architecture underlying HPPD-inhibitor resistance in a Nebraska Amaranthus tuberculatus population
Authors: Murphy Brent P , Beffa Roland , Tranel Patrick J 
Published: June 2021

ABSTRACTBACKGROUNDAmaranthus tuberculatus is a primary driver weed species throughout the American Midwest. Inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD) are an important chemistry for weed management in numerous cropping systems. Here, we characterize the genetic architecture underlying the HPPD-inhibitor resistance trait in an A. tuberculatus population (NEB).RESULTSDose-response studies of an F1 generation identified HPPD-inhibitor resistance as a dominant trait with a resistance/sensitive ratio of 15.0-21.1. Segregation analysis in a pseudo-F2 generation determined the trait is moderately heritable (H2 = 0.556), and complex. Bulk segregant analysis and validation with molecular markers identified two quantitative trait loci (QTL), one on each of Scaffold 4 and 12.CONCLUSIONSResistance to HPPD-inhibitors is a complex, largely dominant trait within the NEB population. Two large-effect QTL were identified controlling HPPD-inhibitor resistance in A. tuberculatus. This is the first QTL mapping study to characterize herbicide resistance in a weedy species.
Elucidation of Structural Mechanism of ATP Inhibition at the AAA1 Subunit of Cytoplasmic Dynein 1 Using a Chemical “Toolkit”
Authors: Tati Sayi’Mone , Alisaraie Laleh 
Published: June 2021

AbstractDynein is a cytoskeletal motor protein that carries organelles via retrograde transport in eukaryotic cells. The motor protein belongs to the ATPase family of proteins associated with diverse cellular activities and plays a critical role in transporting cargoes to the minus end of the microtubules. The motor domain of dynein possesses a hexameric head, where ATP hydrolysis occurs. The AAA1 binding site is the leading ATP hydrolytic site, followed by the AAA3 subsite. Small-molecule ATP competitive inhibitors of dynein are thought to target the AAA1 site. The presented work elucidates the structure-activity relationship of dynapyrazole A and B, ciliobrevin A and D in their various protonated states and their 46 analogs for their binding properties in the nucleotide-binding site of the AAA1 subunit and their effects on the functionally essential subsites of the motor domain of cytoplasmic dynein 1, as there is currently no similar experimental structural data available. Ciliobrevin and its analogs bind to the ATP motifs of the AAA1, namely the Walker-A or P-loop, the Walker-B, and the sensor I and II. Ciliobrevin A shows a better binding affinity to the AAA1 binding site of dynein 1 than its D analog. Although the double bond in ciliobrevin A and D was expected to decrease the ligand potency, they show a better affinity to the AAA1 binding site than dynapyrazole A and B, lacking the bond. Protonation of the nitrogen in ciliobrevin A, D, dynapyrazole A, and B at the N9 site of ciliobrevin, and the N7 of the latter increased their binding affinity. Exploring ciliobrevin A geometrical configuration suggests the E isomer has a superior binding profile over the Z due to binding at the critical ATP motifs. Utilizing the refined structure of the motor domain obtained through protein conformational search in this study exhibits that Arg1852 of the yeast cytoplasmic dynein could involve in the “glutamate switch” mechanism in cytoplasmic dynein 1 in lieu of the conserved Asn in AAA+ protein family, as the guanidine moiety of the Arg engages in an H-bond with the carboxylate moiety of Glu1849.
GLO-SLAM: a slam system optimally combining GPS and LiDAR odometry
Authors: Lin Ruihao , Xu Junzhe , Zhang Jianhua 
Published: June 2021

Purpose
Large-scale and precise three-dimensional (3D) map play an important role in autonomous driving and robot positioning. However, it is difficult to get accurate poses for mapping. On one hand, the global positioning system (GPS) data are not always reliable owing to multipath effect and poor satellite visibility in many urban environments. In another hand, the LiDAR-based odometry has accumulative errors. This paper aims to propose a novel simultaneous localization and mapping (SLAM) system to obtain large-scale and precise 3D map.

Design/methodology/approach
The proposed SLAM system optimally integrates the GPS data and a LiDAR odometry. In this system, two core algorithms are developed. To effectively verify reliability of the GPS data, VGL (the abbreviation of Verify GPS data with LiDAR data) algorithm is proposed and the points from LiDAR are used by the algorithm. To obtain accurate poses in GPS-denied areas, this paper proposes EG-LOAM algorithm, a LiDAR odometry with local optimization strategy to eliminate the accumulative errors by means of reliable GPS data.

Findings
On the KITTI data set and the customized outdoor data set, the system is able to generate high-precision 3D map in both GPS-denied areas and areas covered by GPS. Meanwhile, the VGL algorithm is proved to be able to verify reliability of the GPS data with confidence and the EG-LOAM outperform the state-of-the-art baselines.

Originality/value
A novel SLAM system is proposed to obtain large-scale and precise 3D map. To improve the robustness of the system, the VGL algorithm and the EG-LOAM are designed. The whole system as well as the two algorithms have a satisfactory performance in experiments.
Clothing Economy and Clothing Culture: The Farm Wardrobe from a Gendered Perspective in Nineteenth‐Century Sweden
Authors: Ulväng Marie 
Published: June 2021

AbstractThis article examines the clothing of landowning farmers in inland Swedish from a gendered perspective during industrialisation in the nineteenth century. It considers clothes as possessions and goods within a European framework of trade and influence. In particular, it shows how clothing was a means of expression that changed during the course of industrialisation and how gender became an important factor in the supply and making of clothes. In the region examined, clothing changed from being a local fashion, characterised by similarities in material and workmanship between men's and women's clothes, to become a part of fashion in general with its emphasis on differences between men's and women's wardrobes. In the early nineteenth century, the female wardrobe accounted for a higher value as it included a greater share of garments made of manufactured fabrics. In late nineteenth century, when industrial forestry had replaced livestock farming as the main source of income in the area, men's wardrobes grew in value due to increased demand for tailor‐made garments and purchased fabrics. By contrast, women's garments were often made of simpler fabrics and sewn by seamstresses. These changes responded to the growing breadwinner–homemaker ideal and to national‐romantic ideas about folk costume – two tendencies that emphasised female domesticity and home‐woven fabrics.
Identification of novel pleiotropic gene for bone mineral density and lean mass using the cFDR method
Authors: Tan Li‐Jun , Li Xiao‐Hua , Li Gai‐Gai , Hu Yuan , Chen Xiang‐Ding , Deng Hong‐Wen 
Published: June 2021

AbstractBone mineral density (BMD) and whole‐body lean mass (WBLM) are two important phenotypes of osteoporosis and sarcopenia. Previous studies have shown that BMD and lean mass were phenotypically and genetically correlated. To identify the novel common genetic factors shared between BMD and WBLM, we performed the conditional false discovery rate (cFDR) analysis using summary data of the genome‐wide association study of femoral neck BMD (n = 53,236) and WBLM (n = 38,292) from the Genetic Factors for Osteoporosis Consortium (GEFOS). We identified eight pleiotropic Single Nucleotide Polymorphism (SNPs) (PLCL1 rs11684176 and rs2880389, JAZF1 rs198, ADAMTSL3 rs10906982, RFTN2/MARS2 rs7340470, SH3GL3 rs1896797, ST7L rs10776755, ANKRD44/SF3B1 rs11888760) significantly associated with femoral neck BMD and WBLM (ccFDR < 0.05). Bayesian fine‐mapping analysis showed that rs11888760, rs198, and rs1896797 were the possible functional variants in the ANKRD44/SF3B1, JAZF1i, and SH3GL3 loci, respectively. Functional annotation suggested that rs11888760 was likely to comprise a DNA regulatory element and linked to the expression of RFTN2 and PLCL1. PLCL1 showed differential expression in laryngeal posterior cricoarytenoid muscle between rats of 6 months and 30 months of age. Our findings, together with PLCL1’s potential functional relevance to bone and skeletal muscle function, suggested that rs11888760 was the possible pleiotropic functional variants appearing to coregulate both bone and muscle metabolism through regulating the expression of PLCL1. The findings enhanced our knowledge of genetic associations between BMD and lean mass and provide a rationale for subsequent functional studies of the implicated genes in the pathophysiology of diseases, such as osteoporosis and sarcopenia.
Assessment of seminal calcium and magnesium levels in infertile men with varicocele before and after varicocelectomy
Authors: Azab Sherif Salah , Mostafa Taymour , Abougabal Khadiga M. , Tohamy Asmaa A. , Nabil Nashaat 
Published: June 2021

AbstractBackgroundTrace elements perform a vital role in all stages of human physiology, as well as reproduction.ObjectiveThis study aimed to assess seminal calcium (Ca) and magnesium (Mg) in infertile men associated with varicocele (Vx).Materials and methodsOverall, 50 men were divided into two groups: fertile men (n = 20) and infertile men who were scheduled for Vx surgical repair (n = 30). Exclusion criteria were as follows: azoospermia, smoking, leukocytospermia, and consumption of Ca and/or Mg supplements. All cases were subjected to history taking and clinical examination. Semen analysis and assessment of seminal Ca and Mg by the colorimetric method were carried out for all cases at the base point and 3 months postvaricocelectomy.ResultsGenerally, the mean seminal Ca and Mg levels demonstrated significant decreases in infertile men with Vx compared with the healthy fertile men linked to higher Vx grade as well as Vx bilaterality. These seminal decreases demonstrated significant increases after Vx surgical repair. Collectively, seminal Ca and Mg levels showed a significant positive correlation (r = 0.665, p= 0.001). Besides, seminal Ca, Mg levels, and Ca/Mg ratio showed significant positive correlation with sperm concentration (r = 0.479, p = 0.001; r = 0.541, p = 0.001; r = 0.282, p = 0.001, respectively), sperm motility percentage (r = 0.493, p = 0.001; r = 0.477, p = 0.001; r = 0.353, P = 0.001, respectively), and sperm normal forms percentage (r = 0.578, p = 0.001; r = 0.520, p = 0.001; r = 0.430, p = 0.001, respectively).Discussion and conclusionSeminal Ca and Mg levels and Ca/Mg ratio are significantly decreased in infertile men associated with Vx compared with fertile men with significant increases after varicocelectomy.
Suicide prevention in older adults
Authors: de Mendonça Lima Carlos Augusto , De Leo Diego , Ivbijaro Gabriel , Svab Igor 
Published: June 2021

AbstractBackgroundSuicide among older adults is a multifactorial problem with several interrelated factors involved that vary with age, gender and culture. The number of suicides is highest in those aged 70 years or older in almost all regions of the world. With the increase in life expectancy, and the decrease in mortality due to other causes of death, we could expect the absolute number of older adults' suicide continue increasing.MethodsReview of the literature on suicide protective factors of suicide among older adults.ResultsImprovements on social determinants of health and the timely detection and early treatment of affective disorders are key interventions. Prevention based on community actions and training of gatekeepers may have positive impact. Community programs that promote a sense of usefulness, belonging and that contribute to preserve social integration should be encouraged. Governments should develop the improvement of retirement programs and the development of support systems. The access to general health and mental health services should be facilitated and Primary Care professionals should receive proper training to detect and manage older persons at risk. Actively promoting a culture of coping to different stages of life and to the changes imposed by the advancing of age should form the essential part of a process bringing to better successful aging avenues.ConclusionsSuicide prevention in older adults should broaden its focus and pay attention to the many socio‐environmental conditions that may be relevant in older age, especially social isolation, financial security and physical health.
Psoriasis is associated with fissured tongue but not geographic tongue: a prospective, cross‐sectional, case‐control study
Authors: Monshi Babak , Grabovac Sofia , Gulz Lilli , Ellersdorfer Christina , Vujic Marin , Richter Leo , Kivaranovic Danijel , Rappersberger Klemens , Vujic Igor 
Published: June 2021

SummaryBackground and objectivesIt has been postulated that psoriasis is associated with tongue lesions and geographic tongue might be “oral psoriasis”. However, reports are inconclusive, prevalence rates vary and data for Europe are sparse. In this prospective case‐control study we investigated the point‐prevalence of tongue conditions in an Austrian cohort.Patients and methodsPsoriasis patients and healthy volunteers were assessed regarding tongue and skin lesions, age, sex, smoking habits, allergies, onset of psoriasis, PASI scores and anti‐psoriatic treatment.ResultsWe included 173 psoriasis patients, 58 women, 115 men (median age: 50 [37–60] years), and 173 volunteers, 79 women, 94 men (median age: 54 [43–64] years). Overall, 95 subjects had allergies, 64 psoriasis patients and 50 volunteers were smokers. Median age at onset of psoriasis was 26 (12–40) years, the median PASI score was 2 (0–4.1), most patients received ustekinumab (n = 47). Fissured tongue was significantly associated with psoriasis (25 [14.4 %] psoriasis patients, 13 [7.5 %] volunteers; P = 0.04). Geographic tongue was present in four individuals of each group (2.3%) and associated with smoking (P = 0.01) but not with psoriasis.ConclusionsOverall, we found a low point‐prevalence of tongue lesions in this Austrian cohort. Psoriasis was associated with fissured tongue but not with geographic tongue. Thus, we cannot corroborate the hypothesis that geographic tongue is an oral manifestation of psoriasis.
Invasive validation of the left ventricular global longitudinal strain for estimating left ventricular filling pressure
Authors: Tan Turkan Seda , Turan Serifler Nazli , Demirtola Ayse Irem , Akbulut Irem Muge , Ozyuncu Nil , Vurgun Veysel Kutay , Esenboga Kerim , Kurklu Haci Ali , Kozluca Volkan , Gerede Uludag Demet Menekse , Ongun Aydan , Dincer Irem 
Published: June 2021

AbstractPurposeAn elevated left ventricular (LV) filling pressure is the main finding in heart failure patients with preserved ejection fraction, which is estimated with an algorithm using echocardiographic parameters recommended by the recent American Society of Echocardiography (ASE)/European Association of Cardiovascular Imaging (EACVI) guidelines. In this study, we sought to determine the efficacy of the LV global longitudinal strain (GLS) in predicting an elevated LV filling pressure.Methods and ResultsA total of 73 prospectively selected patients undergoing LV catheterization (mean age 63.19 ± 9.64, 69% male) participated in this study. Using the algorithm, the LV filling pressure was estimated using the echocardiographic parameters obtained within 24 hours before catheterization. The LV GLS was measured using an automated functional imaging system (GE, Vivid E9 USA). Invasive LV pre‐A pressure corresponding to the mean left atrial pressure (LAP) was used as a reference, and a LAP of >12 mm Hg was defined as elevated.Invasive LV filling pressure was elevated in 43 patients (59%) and normal in 30 patients (41%). Nine of 73 (12%) patients were defined as indeterminate based on the 2016 algorithm. Using the ROC method, −18.1% of LV GLS determined the elevated LAP (AUC =0.79; specificity, 73%; sensitivity, 84%) with better sensitivity compared to that by the algorithm (AUC =0.76; specificity, 77%; sensitivity, 72%).ConclusionsWe demonstrated that LV GLS was an independent predictor of elevated LAP as the E/e’ ratio and TR jet velocity and may be used as a major criterion for the diagnosis of HFpEF.
Supporting the adaptive capacity of species through more effective knowledge exchange with conservation practitioners
Authors: Cook Carly N. , Beever Erik A. , Thurman Lindsey L. , Thompson Laura M. , Gross John E. , Whiteley Andrew R. , Nicotra Adrienne B. , Szymanski Jennifer A. , Botero Carlos A. , Hall Kimberly R. , Hoffmann Ary A. , Schuurman Gregor W. , Sgrò Carla M. 
Published: June 2021

AbstractThere is an imperative for conservation practitioners to help biodiversity adapt to accelerating environmental change. Evolutionary biologists are well‐positioned to inform the development of evidence‐based management strategies that support the adaptive capacity of species and ecosystems. Conservation practitioners increasingly accept that management practices must accommodate rapid environmental change, but harbour concerns about how to apply recommended changes to their management contexts. Given the interest from both conservation practitioners and evolutionary biologists in adjusting management practices, we believe there is an opportunity to accelerate the required changes by promoting closer collaboration between these two groups. We highlight how evolutionary biologists can harness lessons from other disciplines about how to foster effective knowledge exchange to make a substantive contribution to the development of effective conservation practices. These lessons include the following: (1) recognizing why practitioners do and do not use scientific evidence; (2) building an evidence base that will influence management decisions; (3) translating theory into a format that conservation practitioners can use to inform management practices; and (4) developing strategies for effective knowledge exchange. Although efforts will be required on both sides, we believe there are rewards for both practitioners and evolutionary biologists, not least of which is fostering practices to help support the long‐term persistence of species.
Using semantic annotations on political debate videos for building open government based lawmaking
Authors: Sánchez‐Nielsen Elena , Chávez‐Gutiérrez Francisco 
Published: June 2021

AbstractThe traditional concept and practice of lawmaking is being transformed by the open government paradigm. It involves a new digital model that links three principles: transparency, participation and collaboration. However, the information system‐enabled solutions in this context are still in their infancy, which none available that address the three crucial challenges: (1) providing the functionalities for semantic search and retrieval of video clips on political debates about legislative proposals, (2) support of open participation and collaboration with contextual information in lawmaking processes and, (3) automatic generation of outcomes achieved in the lawmaking lifecycle. In this article, we describe the underlying methods and techniques leading to address these challenges when a semantic‐driven approach is applied for the development and validation of the real‐world scenario of open government‐based lawmaking systems. To evaluate the value created by using a semantic‐driven approach as computational backbone, the development and testing of a software platform as case study was carried out together with experts from a parliamentary institution and analysed the user experience in parliamentary settings with end‐users. The results show how the use of the appropriate techniques for the adoption of a semantic assumption is a key enabler for the management and retrieval of information tasks relating to the lawmaking lifecycle providing a transparent, participatory and collaborative approach in a holistic way.
Understanding the Factors Associated with Overprotective Parenting Behaviors in Latino Parents
Authors: Suarez‐Morales Lourdes , Torres Cynthia 
Published: June 2021

ObjectiveThe current investigation examined whether parental psychopathology and psychosocial stress are related to overprotective parenting behaviors in a sample of Latino parents.BackgroundScant literature exists on the relationship among psychosocial stress, parental psychopathology, and parental behaviors in Latino populations. Further, the literature has focused on parental behaviors as predictors and not as outcomes.MethodSixty‐four Latino parents participated in this study. Parents completed measures of psychosocial stress, psychopathology, and overprotective parenting behaviors. Regression analyses examined predictors of overprotective parenting behaviors consisting of control, supervision, and separation problems.ResultsFindings showed that psychosocial stress predicted overprotective parenting behaviors. However, parental psychopathology had no effect on parental behaviors. Further analysis of psychosocial stress subscales indicated that immigration stress was associated with parental control and parental separation problems. With regard to subscales of parental psychopathology, parental hostility and parental depression were related to parental control.ConclusionLatino parents' use of controlling parenting behaviors seem to be associated with the level of stress they experience—in particular, immigration‐related stress.ImplicationsThese findings suggest the need for interventions that not only focus on teaching appropriate parenting techniques but that also address psychosocial stressors experienced by Latino parents to diminish the effect that immigration related stress has on their parenting practices.