bluuebunny/crossref_metadata_2025_split huggingface dataset:
The in-utero experience of piglets born from sows with lameness shapes their life trajectory
Authors: Parada Sarmiento Marisol , Bernardino Thiago , Tatemoto Patricia , Polo Gina , Zanella Adroaldo José 
Published: June 2021

AbstractExperiences during gestation can alter the mother’s behavior and physiology, thereby potentially affecting the behavioral and physiological development of the offspring. In livestock, one common challenge for pregnant animals is lameness: a multifactorial condition that causes pain, stress, resulting in poor welfare outcomes. Since maternal pain can affect offspring development, we aimed to quantify the behavioral response in 142 piglets born from sows with different degrees of lameness during pregnancy. Gait scores of 22 pregnant group-housed sows were assessed six times at 2-week intervals. Lameness scores varied from 0 (no lameness) to 5 (most severe lameness score). Saliva samples and behavior were assessed in the sows throughout pregnancy. Sows were moved to individual farrowing pens and placental tissue was collected for glucocorticoid assessment. At 28 days of age, piglets were weaned, weighed, and regrouped by body size and sex. Skin lesions were counted for each piglet on days 28, 29, and 30 after birth. During open field and novel object tests on day 30, the vocalization and activity levels were evaluated. Piglet data were grouped by the lameness score of the sows as G1 (without lameness), G2 (moderate lameness), and G3 (severe lameness). Data analysis included ANOVA or Kruskal–Wallis tests and pairwise comparisons which were performed using Tukey and Kramer (Nemenyi) test with Tukey-Dist approximation for independent samples. G2 piglets were heavier than G3 at weaning. G1 piglets had fewer skin lesions at days 28 and 29 than G2 piglets. Moreover, G1 piglets vocalized more than G2 when they were subjected to the combined open field and novel object test. We did not identify differences among sows showing different lameness scores in the concentration of placental or salivary glucocorticoids. Lameness in pregnant sows altered the offspring’s weight gain, number of skin lesions and vocalizations, together showing evidence that lameness in sows affect offspring performance and behavior.
Impact of a bacterial consortium on the soil bacterial community structure and maize (Zea mays L.) cultivation
Authors: Afanador-Barajas Laura N. , Navarro-Noya Yendi E. , Luna-Guido Marco L. , Dendooven Luc 
Published: June 2021

AbstractMicroorganisms are often applied as biofertilizer to crops to stimulate plant growth, increase yields and reduce inorganic N application. The survival and proliferation of these allochthonous microorganisms in soil is a necessary requisite for them to promote plant growth. We applied a sterilized or unsterilized not commercialized bacterial consortium mixed with cow manure leachate used by a farmer as biofertilizer to maize (Zea mays L.) in a greenhouse experiment, while maize development and the bacterial community structure was determined just before the biofertilizer was applied a first time (day 44), after three applications (day 89) and after six application at the end of the experiment (day 130). Application of sterilized or unsterilized biofertilizer with pH 4.3 and 864 mg NH4+-N kg−1 had no significant effect on maize growth. The application of the biofertilizer dominated by Lactobacillus (relative abundance 11.90%) or the sterilized biofertilizer changed the relative abundance of a limited number of bacterial groups, i.e. Delftia, Halomonas, Lactobacillus and Stenotrophomonas, without altering significantly the bacterial community structure. Cultivation of maize, however, affected significantly the bacterial community structure, which showed large significant variations over time in the cultivated and uncultivated soil. It was concluded that the bacteria applied as a biofertilizer had only a limited effect on the relative abundance of these groups in uncultivated or soil cultivated with maize.
Detection of the location of pneumothorax in chest X-rays using small artificial neural networks and a simple training process
Authors: Cho Yongil , Kim Jong Soo , Lim Tae Ho , Lee Inhye , Choi Jongbong 
Published: June 2021

AbstractThe purpose of this study was to evaluate the diagnostic performance achieved by using fully-connected small artificial neural networks (ANNs) and a simple training process, the Kim-Monte Carlo algorithm, to detect the location of pneumothorax in chest X-rays. A total of 1,000 chest X-ray images with pneumothorax were taken randomly from NIH (the National Institutes of Health) public image database and used as the training and test sets. Each X-ray image with pneumothorax was divided into 49 boxes for pneumothorax localization. For each of the boxes in the chest X-ray images contained in the test set, the area under the receiver operating characteristic (ROC) curve (AUC) was 0.882, and the sensitivity and specificity were 80.6% and 83.0%, respectively. In addition, a common currently used deep-learning method for image recognition, the convolution neural network (CNN), was also applied to the same dataset for comparison purposes. The performance of the fully-connected small ANN was better than that of the CNN. Regarding the diagnostic performances of the CNN with different activation functions, the CNN with a sigmoid activation function for fully-connected hidden nodes was better than the CNN with the rectified linear unit (RELU) activation function. This study showed that our approach can accurately detect the location of pneumothorax in chest X-rays, significantly reduce the time delay incurred when diagnosing urgent diseases such as pneumothorax, and increase the effectiveness of clinical practice and patient care.
The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity
Authors: Nguyen Dinh Thi , Le Thuong Manh , Hattori Tsuyoshi , Takarada-Iemata Mika , Ishii Hiroshi , Roboon Jureepon , Tamatani Takashi , Kannon Takayuki , Hosomichi Kazuyoshi , Tajima Atsushi , Taniuchi Shusuke , Miyake Masato , Oyadomari Seiichi , Tanaka Takashi , Kato Nobuo , Saito Shunsuke , Mori Kazutoshi , Hori Osamu 
Published: June 2021

AbstractWhile ATF6α plays a central role in the endoplasmic reticulum (ER) stress response, the function of its paralogue ATF6β remains elusive, especially in the central nervous system (CNS). Here, we demonstrate that ATF6β is highly expressed in the hippocampus of the brain, and specifically regulates the expression of calreticulin (CRT), a molecular chaperone in the ER with a high Ca2+-binding capacity. CRT expression was reduced to ~ 50% in the CNS of Atf6b−/− mice under both normal and ER stress conditions. Analysis using cultured hippocampal neurons revealed that ATF6β deficiency reduced Ca2+ stores in the ER and enhanced ER stress-induced death. The higher levels of death in Atf6b−/− neurons were recovered by ATF6β and CRT overexpressions, or by treatment with Ca2+-modulating reagents such as BAPTA-AM and 2-APB, and with an ER stress inhibitor salubrinal. In vivo, kainate-induced neuronal death was enhanced in the hippocampi of Atf6b−/− and Calr+/− mice, and restored by administration of 2-APB and salubrinal. These results suggest that the ATF6β-CRT axis promotes neuronal survival under ER stress and excitotoxity by improving intracellular Ca2+ homeostasis.
Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells
Authors: Eroglu Binnur , Genova Evan , Zhang Quanguang , Su Yun , Shi Xingming , Isales Carlos , Eroglu Ali 
Published: June 2021

AbstractThe plasticity and proliferative capacity of stem cells decrease with aging, compromising their tissue regenerative potential and therapeutic applications. This decline is directly linked to mitochondrial dysfunction. Here, we present an effective strategy to reverse aging of mouse bone marrow mesenchymal stem cells (BM-MSCs) by restoring their mitochondrial functionality using photobiomodulation (PBM) therapy. Following the characterization of young and aged MSCs, our results show that a near-infrared PBM treatment delivering 3 J/cm2 is the most effective modality for improving mitochondrial functionality and aging markers. Furthermore, our results unveil that young and aged MSCs respond differently to the same modality of PBM: whereas the beneficial effect of a single PBM treatment dissipates within 7 h in aged stem cells, it is lasting in young ones. Nevertheless, by applying three consecutive treatments at 24-h intervals, we were able to obtain a lasting rejuvenating effect on aged MSCs. Our findings are of particular significance for improving autologous stem cell transplantation in older individuals who need such therapies most.
The first genotype II African swine fever virus isolated in Africa provides insight into the current Eurasian pandemic
Authors: Njau Emma P. , Domelevo Entfellner Jean-Baka , Machuka Eunice M. , Bochere Edwina N. , Cleaveland Sarah , Shirima Gabriel M. , Kusiluka Lughano J. , Upton Chris , Bishop Richard P. , Pelle Roger , Okoth Edward A. 
Published: June 2021

AbstractAfrican swine fever (ASF) caused by the African swine fever virus (ASFV) is ranked by OIE as the most important source of mortality in domestic pigs globally and is indigenous to African wild suids and soft ticks. Despite two ASFV genotypes causing economically devastating epidemics outside the continent since 1961, there have been no genome-level analyses of virus evolution in Africa. The virus was recently transported from south-eastern Africa to Georgia in 2007 and has subsequently spread to Russia, eastern Europe, China, and south-east Asia with devastating socioeconomic consequences. To date, two of the 24 currently described ASFV genotypes defined by sequencing of the p72 gene, namely genotype I and II, have been reported outside Africa, with genotype II being responsible for the ongoing pig pandemic. Multiple complete genotype II genome sequences have been reported from European, Russian and Chinese virus isolates but no complete genome sequences have yet been reported from Africa. We report herein the complete genome of a Tanzanian genotype II isolate, Tanzania/Rukwa/2017/1, collected in 2017 and determined using an Illumina short read strategy. The Tanzania/Rukwa/2017/1 sequence is 183,186 bp in length (in a single contig) and contains 188 open reading frames. Considering only un-gapped sites in the pairwise alignments, the new sequence has 99.961% identity with the updated Georgia 2007/1 reference isolate (FR682468.2), 99.960% identity with Polish isolate Pol16_29413_o23 (MG939586) and 99.957% identity with Chinese isolate ASFV-wbBS01 (MK645909.1). This represents 73 single nucleotide polymorphisms (SNPs) relative to the Polish isolate and 78 SNPs with the Chinese genome. Phylogenetic analysis indicated that Tanzania/Rukwa/2017/1 clusters most closely with Georgia 2007/1. The majority of the differences between Tanzania/Rukwa/2017/1 and Georgia 2007/1 genotype II genomes are insertions/deletions (indels) as is typical for ASFV. The indels included differences in the length and copy number of the terminal multicopy gene families, MGF 360 and 110. The Rukwa2017/1 sequence is the first complete genotype II genome from a precisely mapped locality in Africa, since the exact origin of Georgia2007/1 is unknown. It therefore provides baseline information for future analyses of the diversity and phylogeography of this globally important genetic sub-group of ASF viruses.
Scalable 18,650 aqueous-based supercapacitors using hydrophobicity concept of anti-corrosion graphite passivation layer
Authors: Chomkhuntod Praeploy , Iamprasertkun Pawin , Chiochan Poramane , Suktha Phansiri , Sawangphruk Montree 
Published: June 2021

AbstractScalable aqueous-based supercapacitors are ideal as future energy storage technologies due to their great safety, low cost, and environmental friendliness. However, the corrosion of metal current collectors e.g., aluminium (Al) foil in aqueous solutions limits their practical applications. In this work, we demonstrate a low-cost, scalable, and simple method to prepare an anti-corrosion current collector using a concept of hydrophobicity by coating the hydrophobic graphite passivation layer on the Al foil via a roll-to-roll coating technology at the semi-automation scale of production pilot plant of 18,650 cylindrical supercapacitor cells. All qualities of materials, electrodes, and production process are therefore in the quality control as the same level of commercial supercapacitors. In addition, the effects of the graphite coating layer have been fundamentally evaluated. We have found that the graphite-coated layer can improve the interfacial contact without air void space between the activated carbon active material layer and the Al foil current collector. Importantly, it can suppress the corrosion and the formation of resistive oxide film resulting in better rate capability and excellent cycling stability without capacitance loss after long cycling. The scalable supercapacitor prototypes here in this work may pave the way to practical 18,650 supercapacitors for sustainable energy storage systems in the future.
Metabolomic and lipidomic changes triggered by lipopolysaccharide-induced systemic inflammation in transgenic APdE9 mice
Authors: Puris Elena , Kouřil Štěpán , Najdekr Lukáš , Loppi Sanna , Korhonen Paula , Kanninen Katja M. , Malm Tarja , Koistinaho Jari , Friedecký David , Gynther Mikko 
Published: June 2021

AbstractPeripheral infections followed by systemic inflammation may contribute to the onset of Alzheimer`s disease (AD) and accelerate the disease progression later in life. Yet, the impact of systemic inflammation on the plasma and brain tissue metabolome and lipidome in AD has not been investigated. In this study, targeted metabolomic and untargeted lipidomic profiling experiments were performed on the plasma, cortices, and hippocampi of wild-type (WT) mice and transgenic APdE9 mice after chronic lipopolysaccharide (LPS) treatment, as well as saline-treated APdE9 mice. The lipidome and the metabolome of these mice were compared to saline-treated WT animals. In the brain tissue of all three models, the lipidome was more influenced than the metabolome. The LPS-treated APdE9 mice had the highest number of changes in brain metabolic pathways with significant alterations in levels of lysine, myo-inositol, spermine, phosphocreatine, acylcarnitines and diacylglycerols, which were not observed in the saline-treated APdE9 mice. In the WT mice, the effect of the LPS administration on metabolome and lipidome was negligible. The study provided exciting information about the biochemical perturbations due to LPS-induced inflammation in the transgenic AD model, which can significantly enhance our understanding of the role of systemic inflammation in AD pathogenesis.
TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
Authors: Javadirad Seyed-Morteza , Mokhtari Mohammad 
Published: June 2021

AbstractThe association of PRM1/2 with male azoospermia is well-documented, but the relationship between TXNDC2 deficiency and the azoospermia phenotype, sperm retrieval, and pathology has not been elucidated. Here we identified the association of TXNDC2 and protamines in evaluating testis pathology and sperm retrieval. An extensive microarray meta-analysis of men with idiopathic azoospermia was performed, and after undergoing several steps of data quality controls, the data passing QC were pooled and batch effect corrected. As redox imbalance has been shown to have a variable relationship with fertility, our relative expression studies began with candidate protamination and thioredoxin genes. We constructed a logistic regression model of TXNDC2 with PRM1 and PRM2 genes, and collective ROC analysis indicated a sensitivity of 96.8% and specificity of 95.5% with a ROC value of 0.995 (SE = 0.0070, 95% CI 0.982–1.000). These results demonstrate that TXNDC2, PRM1, and PRM2 combined have a robust power to predict sperm retrieval and correlate with severe azoospermia pathology.
A cohort study to investigate sex-specific differences in ANCA-associated glomerulonephritis outcomes
Authors: Scott Jennifer , Canepa Carolina , Buettner Antonia , Ryan Louise , Moloney Bróna , Cormican Sarah , Walsh Cathal , White Arthur , Salama Alan D. , Little Mark A. 
Published: June 2021

AbstractData surrounding sex-specific differences in ANCA-associated vasculitis glomerulonephritis (ANCA-GN) outcomes is sparse. We hypothesised that the previously observed increased risk of end-stage kidney disease (ESKD) in males is driven by sex-specific variation in immunosuppression dosing. Patients were recruited to the Irish Rare Kidney Disease Registry or followed by the Royal Free Hospital vasculitis team (2012–2020). Inclusion criteria: prior diagnosis of ANCA-GN (biopsy proven pauci-immune glomerulonephritis) and positive serology for anti-MPO or -PR3 antibodies. Renal and patient survival, stratified by sex and Berden histological class, was analysed. The cumulative- and starting dose/kilogram of induction agents and prednisolone, respectively, was compared between sexes. 332 patients were included. Median follow-up was time 40.2 months (IQR 17.3–69.2). 73 (22%) reached ESKD and 47 (14.2%) died. Overall 1- and 5-year renal survival was 82.2% and 76.7% in males and 87.1% and 82.0% in females, respectively (p 0.13). The hazard ratio for ESKD in males versus females, after adjustment for age, ANCA serology, baseline creatinine and histological class was 1.07 (95% CI 0.59–1.93). There was no difference between sexes in the dose/kilogram of any induction agent. We did not observe a strong impact of sex on renal outcome in ANCA-GN. Treatment intensity does not vary by sex.
A possible relation between Arctic sea ice and late season Indian Summer Monsoon Rainfall extremes
Authors: Chatterjee Sourav , Ravichandran Muthalagu , Murukesh Nuncio , Raj Roshin P. , Johannessen Ola M. 
Published: June 2021

AbstractThe out-of-phase inter-decadal co-variability between summer (JJA) sea ice extent (SIE) in the Kara Sea (KS) sector of the Arctic Ocean and Indian Summer Monsoon Rainfall (ISMR) is found to be weakened during the recent decades with rapidly declining SIE in the KS (since the 1980s). However, SIE in the KS and frequency of ISMR extremes are found to have a consistent out-of-phase relation during the rapidly declining SIE periods. A possible physical mechanism for the relation between the late-season ISMR extremes and summer SIE in the KS is suggested, focusing on the recent years since the 1980s.
Exploring the evidence for epigenetic regulation of environmental influences on child health across generations
Authors: Breton Carrie V. , Landon Remy , Kahn Linda G. , Enlow Michelle Bosquet , Peterson Alicia K. , Bastain Theresa , Braun Joseph , Comstock Sarah S. , Duarte Cristiane S. , Hipwell Alison , Ji Hong , LaSalle Janine M. , Miller Rachel L. , Musci Rashelle , Posner Jonathan , Schmidt Rebecca , Suglia Shakira F. , Tung Irene , Weisenberger Daniel , Zhu Yeyi , Fry Rebecca 
Published: June 2021

AbstractEnvironmental exposures, psychosocial stressors and nutrition are all potentially important influences that may impact health outcomes directly or via interactions with the genome or epigenome over generations. While there have been clear successes in large-scale human genetic studies in recent decades, there is still a substantial amount of missing heritability to be elucidated for complex childhood disorders. Mounting evidence, primarily in animals, suggests environmental exposures may generate or perpetuate altered health outcomes across one or more generations. One putative mechanism for these environmental health effects is via altered epigenetic regulation. This review highlights the current epidemiologic literature and supporting animal studies that describe intergenerational and transgenerational health effects of environmental exposures. Both maternal and paternal exposures and transmission patterns are considered, with attention paid to the attendant ethical, legal and social implications.
Systemic inflammation down-regulates glyoxalase-1 expression: an experimental study in healthy males
Authors: G.H. Driessen Rob , Kiers Dorien , G. Schalkwijk Casper , L.J.M. Scheijen Jean , Gerretsen Jelle , Pickkers Peter , C.G. van de Poll Marcel , C.C. van der Horst Iwan , C.J.J. Bergmans Dennis , Kox Matthijs , C.T. van Bussel Bas 
Published: June 2021

AbstractBackground: Hypoxia and inflammation are hallmarks of critical illness, related to multiple organ failure. A possible mechanism leading to multiple organ failure is hypoxia- or inflammation-induced down-regulation of the detoxifying glyoxalase system that clears dicarbonyl stress. The dicarbonyl methylglyoxal (MGO) is a highly reactive agent produced by metabolic pathways such as anaerobic glycolysis and gluconeogenesis. MGO leads to protein damage and ultimately multi-organ failure. Whether detoxification of MGO into D-lactate by glyoxalase functions appropriately under conditions of hypoxia and inflammation is largely unknown. We investigated the effect of inflammation and hypoxia on the MGO pathway in humans in vivo.Methods: After prehydration with glucose 2.5% solution, ten healthy males were exposed to hypoxia (arterial saturation 80–85%) for 3.5 h using an air-tight respiratory helmet, ten males to experimental endotoxemia (LPS 2 ng/kg i.v.), ten males to LPS+hypoxia and ten males to none of these interventions (control group). Serial blood samples were drawn, and glyoxalase-1 mRNA expression, MGO, methylglyoxal-derived hydroimidazolone-1 (MG-H1), D-lactate and L-lactate levels, were measured serially.Results: Glyoxalase-1 mRNA expression decreased in the LPS (β (95%CI); -0.87 (-1.24; -0.50) and the LPS+hypoxia groups; -0.78 (-1.07; -0.48) (P&lt;0.001). MGO was equal between groups, whereas MG-H1 increased over time in the control group only (P=0.003). D-Lactate was increased in all four groups. L-Lactate was increased in all groups, except in the control group.Conclusion: Systemic inflammation downregulates glyoxalase-1 mRNA expression in humans. This is a possible mechanism leading to cell damage and multi-organ failure in critical illness with potential for intervention.
Manufacturing of animal products by the assembly of microfabricated tissues
Authors: Jo Byeongwook , Nie Minghao , Takeuchi Shoji 
Published: June 2021

AbstractWith the current rapidly growing global population, the animal product industry faces challenges which not only demand drastically increased amounts of animal products but also have to limit the emission of greenhouse gases and animal waste. These issues can be solved by the combination of microfabrication and tissue engineering techniques, which utilize the microtissue as a building component for larger tissue assembly to fabricate animal products. Various methods for the assembly of microtissue have been proposed such as spinning, cell layering, and 3D bioprinting to mimic the intricate morphology and function of the in vivo animal tissues. Some of the demonstrations on cultured meat and leather-like materials present promising outlooks on the emerging field of in vitro production of animal products.