bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brazilian invasion and dispersion routes of the cotton boll weevil
Authors: Marquesini Cristina Jensen Vasconcelos , Maggio Daniela Hipólito , Guedes Raul Narciso Carvalho , Correa Alberto Soares 
Published: December 2021

AbstractThe cotton boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), is the major insect pest of cotton in the Americas. Although the demographic and dispersion patterns of the cotton boll weevil in the USA and Mexico were well characterized, in Brazil the information is scarce and does not include the main cotton‐producing regions of the country. Thus, our objective was to apply a phylogeographic approach sequencing a gene fragment of the cytochrome c oxidase subunit I (COI) to estimate the genetic diversity, identify the genetic strains, and describe the dispersion routes of the cotton boll weevil in the Brazilian territory. The COI marker revealed a low genetic diversity of the cotton boll weevil with only two haplotypes in Brazil. The haplotypes are genetically related to individuals from the eastern region of the USA and Mexico, which suggests a recent invasion event in Brazil. The genetic structure of COI haplotypes indicates a low dispersion of boll weevils between north/northeast and midwest/southeast regions of Brazil. The genetic drift and low gene flow during the insect expansion explain the COI haplotype distribution. The genetic structure of the cotton boll weevil in Brazil is novel information and provides valuable insight for the design of suitable pest management practices against this key pest species in the country.
Dietary habits in Australian, New Zealand and Malaysian patients with end stage kidney failure: A pre‐specified cross‐sectional study of the FAVOURED trial participants
Authors: Conley Marguerite , Barden Anne , Viecelli Andrea K. , Irish Ashley B. , Cass Alan , Hawley Carmel M. , Voss David , Pascoe Elaine M. , Lenhoff Katie , Polkinghorne Kevan R. , Hooi Lai‐Seong , Ong Loke‐Meng , Paul‐Brent Peta‐Anne , Kerr Peter G. , Mori Trevor A. ,   the FAVOURED Trial Investigator Team
Published: December 2021

AbstractBackgroundDietary management plays an important role in patients with kidney failure. Current dietary habits of Australians and New Zealanders (ANZ) and Malaysians with chronic kidney disease (CKD Stage 4–5) have not been adequately investigated. We report the dietary habits of people with advanced CKD and their adherence to country‐specific dietary guidelines.MethodsParticipants with CKD Stage 4–5, enrolled in the Omega‐3 Fatty Acids (Fish oils) and Aspirin in Vascular access Outcomes in Renal Disease (FAVOURED) trial, completed a lifestyle questionnaire at baseline on their dietary intake.ResultsOf 567 participants, 538 (ANZ, n = 386; Malaysian, n = 152; mean ± SD age 54.8 ± 14.3 years, 64% male) completed the questionnaire. Dietary fruit and vegetable intakes were higher in ANZ participants; 49% (n = 189) consumed ≥2 serves day–1 of fruit and 61% (n = 235) ate ≥2 serves day–1 of vegetables compared to 24% (n = 36) and 34% (n = 52) of Malaysians, respectively (p < 0.0001). Only 4% (n = 15) of ANZ participants met Australian Dietary recommendations of two fruit and five vegetable serves day–1. Fish consumption was higher in Malaysians with 83% (n = 126) consuming ≥2 serves week–1 compared to 21% (n = 81) of ANZ participants (p < 0.001). Red meat intake was higher in ANZ participants; however, chicken consumption was similar; 48% (n = 185) consumed >2 chicken serves week–1 and 65% (n = 251) ate >2 serves week–1 of red meat compared to 43% (n = 65) and 15% (n = 23) of Malaysians, respectively.ConclusionsSignificant regional variation in dietary intake for fruit, vegetables and animal protein is described that likely reflects cultural and economic differences. Barriers to meeting recommended dietary intakes require further investigation.
Assessment of the self‐healing capacity of cementitious materials through active thin sections
Authors: Rossi Emanuele , Romero Rodriguez Claudia , Jonkers Henk , Çopuroğlu Oğuzhan 
Published: December 2021

AbstractSince self‐healing of cementitious materials can theoretically improve the service‐life of concrete structures, it has gathered significant attention from both researchers and industry during the last two decades. Many researchers have proposed different methods to assess and quantify the self‐healing capacity (i.e. the ability of cementitious materials to heal cracks) that is generated in concrete autogenously as well as autonomously. Even though many methodologies can be found in the literature, a way to accurately quantify the healing products produced by any self‐healing mechanism has not been yet achieved. In this study, a methodology is proposed to observe and to quantify in‐time formation of healing products based on active thin sections. Thin sections of Portland cement paste have been prepared with no epoxy impregnation to facilitate reactions between the cement matrix and the surrounding environment. Artificial cracks (260 μm wide) were induced at 28 days of age and the crystal growth was continuously monitored up to 28 days of self‐healing. Through image analysis of the micrographs, it was calculated that the autogenous self‐healing capacity of paste (triggered by portlandite carbonation in uncontrolled indoor conditions) was around 55% after 28 days of self‐healing. Healing products were further characterised through Environmental Scanning Electron Microscope analysis. Based on the results obtained in this study, the proposed methodology seems to be promising to compare the self‐healing mechanisms triggered by different healing agents.
Fragmented habitats and Pleistocene climate shaped diversification of the hoary bamboo rat (Rhizomys pruinosus) in the mountainous plateau of SW China
Authors: Gao Yong , Yan Yue‐Hui , Yin Si , Yu Long , Zhu Lei , Ding Xue‐Mei , Zhang Ya‐Nan , Chen Huan‐Huan , Tang Li‐Jun , Li Zhu‐Mei , Chu Hong‐Long , Shi Xiao‐Dong , Zou Zheng‐Rong , Tang Li‐Zhou 
Published: December 2021

AbstractThe southwest mountainous region of China has been characterized as one of the worldwide biodiversity hotspots, but mechanisms underlying diversification of organisms in this region are still not clear. We assessed whether fragmented mountainous habitats and Pleistocene climate changes impacted the genetic diversity and diversification patterns of the hoary bamboo rat (Rhizomys pruinosus Blyth), a widely distributed species of rodent in SW China. Genetic diversity analyses were undertaken based on four mitochondrial DNA regions and 12 nuclear microsatellite loci (simple sequence repeats), representing 153 individuals from 24 populations across SW China. Moreover, we investigated correlations between genotype and geographical components, and predicted species distribution models for R. pruinosus under the historical and present climate conditions. Both mitochondrial DNA and simple sequence repeat markers revealed substantial genetic diversity and strong differentiation between populations. Phylogeographical analyses revealed two phylogenetic clades that were consistent with their geographical distributions (eastern and western clades). We inferred that the divergence of R. pruinosus was largely driven by Quaternary climatic oscillations and regionally fragmented mountainous habitats with environmental and geographical heterogeneity. Overall, our study revealed diversification patterns of R. pruinosus—patterns that may be shared by small alpine vertebrates in SW China.
Expiratory activity during sleep in children
Authors: Saini Jasmeen K. , Janes Tara. A. , MacLean Joanna E. , Pagliardini Silvia 
Published: December 2021

AbstractSleep irregularities and respiratory events (apnea, O2 desaturation or a combination thereof) are often present in the infant population. While inspiration is the main active process in the act of breathing, expiration is generally thought to occur passively. Although commonly considered as quiet during sleep, expiratory abdominal muscles have been proposed to be recruited to promote ventilation, facilitate gas exchange, and reduce the work of breathing during conditions of increased respiratory drive, exercise, or airway obstruction. In this study, we investigated the occurrence of expiratory abdominal muscle activity in polysomnographic studies of subjects (aged 0–2 years) suspected of sleep disordered breathing. Our results indicate that abdominal muscle activation occurs during sleep, most frequently during non‐rapid eye movement and rapid‐eye movement states compared to slow‐wave sleep. Furthermore, abdominal muscle activity was present during regular breathing or associated with respiratory events (apneas or O2 desaturation). In the latter case, abdominal muscle recruitment more frequently followed the onset of respiratory events and terminated with recovery from blood O2 desaturation events. We conclude that expiratory abdominal muscle activity contributes to the pattern of respiratory muscle recruitment during sleep in infants and given its temporal relationship with respiratory events, we propose that its recruitment could facilitate proper ventilation by counteracting airway resistance and O2 desaturation in infancy across different stages of sleep.
The plasma membrane H+‐ATPase FgPMA1 regulates the development, pathogenicity, and phenamacril sensitivity of Fusarium graminearum by interacting with FgMyo‐5 and FgBmh2
Authors: Wu Luoyu , Yuan Zhili , Wang Pengwei , Mao Xuewei , Zhou Mingguo , Hou Yiping 
Published: December 2021

AbstractFusarium graminearum, as the causal agent of Fusarium head blight (FHB), not only causes yield loss, but also contaminates the quality of wheat by producing mycotoxins, such as deoxynivalenol (DON). The plasma membrane H+‐ATPases play important roles in many growth stages in plants and yeasts, but their functions and regulation in phytopathogenic fungi remain largely unknown. Here we characterized two plasma membrane H+‐ATPases: FgPMA1 and FgPMA2 in F. graminearum. The FgPMA1 deletion mutant (∆FgPMA1), but not FgPMA2 deletion mutant (∆FgPMA2), was impaired in vegetative growth, pathogenicity, and sexual and asexual development. FgPMA1 was localized to the plasma membrane, and ∆FgPMA1 displayed reduced integrity of plasma membrane. ∆FgPMA1 not only impaired the formation of the toxisome, which is a compartment where DON is produced, but also suppressed the expression level of DON biosynthetic enzymes, decreased DON production, and decreased the amount of mycelial invasion, leading to impaired pathogenicity by exclusively developing disease on inoculation sites of wheat ears and coleoptiles. ∆FgPMA1 exhibited decreased sensitivity to some osmotic stresses, a cell wall‐damaging agent (Congo red), a cell membrane‐damaging agent (sodium dodecyl sulphate), and heat shock stress. FgMyo‐5 is the target of phenamacril used for controlling FHB. We found FgPMA1 interacted with FgMyo‐5, and ∆FgPMA1 showed an increased expression level of FgMyo‐5, resulting in increased sensitivity to phenamacril, but not to other fungicides. Furthermore, co‐immunoprecipitation confirmed that FgPMA1, FgMyo‐5, and FgBmh2 (a 14‐3‐3 protein) form a complex to regulate the sensitivity to phenamacril and biological functions. Collectively, this study identified a novel regulating mechanism of FgPMA1 in pathogenicity and phenamacril sensitivity of F. graminearum.
A new biochemistry connecting pathogen detection to induced defense in plants
Authors: Parker Jane E. , Hessler Giuliana , Cui Haitao 
Published: December 2021

SummaryPlant cell surface and intracellular immune receptors recognizing pathogen attack utilize the same defense machineries to mobilize resistance. New genetic, protein structural and biochemical information on receptor activation and signaling is transforming understanding of how their shared defense network operates. We discuss the biochemical activities of two classes of intracellular nucleotide‐binding/leucine‐rich repeat (NLR) receptor – one forming a Ca2+ channel, the other an NADase enzyme – which define engagement of enhanced disease susceptibility 1 (EDS1)‐family heterodimers and cofunctioning helper NLRs (RNLs) to connect receptor systems and amplify defenses. Toll‐interleukin‐1 receptor (TIR) domain NLR receptors and TIR‐domain proteins, with a capacity to produce NAD+‐derived small molecules, require EDS1 dimers and RNLs for defense induction. The TIR‐driven EDS1/RNL modules emerge as central elements in Ca2+‐based immunity signaling initiated by receptors outside and inside host cells.
Impacts of global change on the phyllosphere microbiome
Authors: Zhu Yong‐Guan , Xiong Chao , Wei Zhong , Chen Qing‐Lin , Ma Bin , Zhou Shu‐Yi‐Dan , Tan Jiaqi , Zhang Li‐Mei , Cui Hui‐Ling , Duan Gui‐Lan 
Published: December 2021

SummaryPlants form complex interaction networks with diverse microbiomes in the environment, and the intricate interplay between plants and their associated microbiomes can greatly influence ecosystem processes and functions. The phyllosphere, the aerial part of the plant, provides a unique habitat for diverse microbes, and in return the phyllosphere microbiome greatly affects plant performance. As an open system, the phyllosphere is subjected to environmental perturbations, including global change, which will impact the crosstalk between plants and their microbiomes. In this review, we aim to provide a synthesis of current knowledge of the complex interactions between plants and the phyllosphere microbiome under global changes and to identify future priority areas of research on this topic.
Beyond the uncanny: Shirley Jackson’s poetics of alienation
Authors: Angeloch Dominic 
Published: December 2021

AbstractShirley Jackson is one of the most important authors of the uncanny of the twentieth century. Forgotten after her death in 1965, she is currently being rediscovered. This paper attributes her mastery to the depiction and evocation of extreme mental states, especially alienation and estrangement from oneself. In the debate on the uncanny dominated by Freud’s essay of the same name, these phenomena, situated at the edges of the uncanny, are usually left out. A reading of Freud’s short text “A disturbance of memory on the Acropolis” (1936), which has so far gone unnoticed in this context, makes it possible to introduce the concepts of “depersonalization” and “derealization” into the conceptual constellation of the uncanny. Against this background, the paper undertakes a close reading of Jackson’s story “The Tooth” (1948). Jackson’s narrative techniques here, as in her later works, are based on a poetics of transgression. The actual subject of the narrative is told by shaping it subliminally: instead of discursively treating the depths of the human psyche, the narrative evokes them directly in its readers. This is the reason why Jackson’s literature is able to convey (self‐)alienation and related phenomena such as dreams and hallucinations so vividly.
The Efficiency of Global Genome‐Nucleotide Excision Repair is Linked to the Fraction of Open rRNA Gene Chromatin, in Yeast†
Authors: Paillé Audrey , Charton Romain , Dholandre Quentin , Conconi Antonio 
Published: December 2021

ABSTRACTThe yeast rDNA locus is a suitable model to study nucleotide excision repair (NER) in chromatin. A portion of rRNA genes is transcribed and largely depleted of nucleosomes, the remaining genes are not transcribed and folded in nucleosomes. In G1‐arrested cells, most rRNA genes do not have nucleosomes. TC‐NER removes UV‐induced DNA lesions from the transcribed strand of active genes. GG‐NER is less efficient and removes DNA lesions from the nontranscribed strand of active genes and from the inactive genome. Different from mammalian cells, in yeast, the rRNA gene‐transcribed strand is repaired by RNA polymerase‐I‐dependent TC‐NER. The opposite nontranscribed strand is repaired faster than both strands of inactive rRNA genes. In log‐phase cells, RNA polymerase‐I are dislodged from the damaged transcribed strand and partially replaced by nucleosomes. Contrary to log‐phase cells, in G1‐phase cells few, if any, histones are deposited on the open rRNA genes during NER. In this study, we compared GG‐NER efficiency in the rRNA gene coding region: without nucleosomes, partially loaded or wholly loaded with nucleosomes. The results indicate that in log‐phase cells histones obstruct GG‐NER, whereas in G1‐phase cells GG‐NER is as efficient as TC‐NER.
The secret of H2S to keep plants young and fresh and its products
Authors: Liu D. , Pei Y. 
Published: December 2021

AbstractRecently, accumulating evidence has shown that hydrogen sulphide (H2S), a newly determined gasotransmitter, plays important roles in senescence, which is an essential biological process for plant fitness and an important agricultural trait that is critical for the yield and quality of farm produce. Here, in this review, we summarize the roles of H2S in senescence, both before and after the harvesting of agricultural products, and the underlying mechanism is also discussed. During the plant growth process, the function of H2S in the leaf senescence process has been studied extensively, and H2S plays roles during the whole process, including the initiation, reorganization and terminal stages. While during the postharvest stage, H2S can prevents farm products from deterioration resulting from over‐ripening, pathogen attack and incorrect storage. The underlying H2S‐related mechanisms during different stages of the senescence process are summarized and compared. The most prominent interaction occurs between H2S and reactive oxygen species, and the molecular mechanism is explored. Additionally, the conserved action mode of H2S in different life processes and different species is also discussed. In the future, multi‐omics analyses over time will be needed to investigate the detailed mechanisms of H2S, and a safety attribute analysis of H2S is also required before it can be used in agricultural production.
Associations between student‐athletes’ self‐esteem and career adaptability across the high school years
Authors: Nikander Jaakko A. O. , Aunola Kaisa , Tolvanen Asko , Ryba Tatiana V. 
Published: December 2021

The key challenges in sustainable elite sports concern young athletes´ holistic development. Consequently, preparation for dual careers has been recommended to enhance athletes’ well‐being and equip them for life after sports. The aim of this study was to examine the developmental associations between self‐esteem and career adaptability among adolescent athletes across the high school years and investigate the role of gender in these developmental trajectories. A total of 391 student‐athletes were followed up four times from the beginning of high school (T1) to its end (T4) using the Dual‐Career Form of the Career Adapt‐Abilities Scale and the Self‐Esteem Questionnaire. The results indicated that individual differences in student‐athletes’ self‐esteem and career adaptability were relatively stable across the high school years and were thus modeled via the between‐persons factors capturing differences between individuals across time. The results further showed that the overall levels of self‐esteem and career adaptability were positively associated. Males showed higher overall levels of career adaptability and self‐esteem than females. This study suggests that it is important to facilitate youth athletes’ self‐regulation resources by involving them in vocational developmental tasks. Further, gender differences should be considered when supporting student‐athletes’ development.
Outcome of dogs with bone marrow suppression secondary to Sertoli cell tumour
Authors: Salyer Sarah A. , Lapsley Janis M. , Palm Carrie A. , Culp William T. N. , Lundberg Alycen P. , Phillips Heidi , McKenna Charly , Oblak Michelle L. , Hall Robyn , Wustefeld‐Janssens Brandan , Tremolada Giovanni , Selmic Laura E. 
Published: December 2021

AbstractSertoli cell tumours are one of the most common canine testicular neoplasia. These tumours are significantly more likely to arise in cryptorchid dogs and are often functional, oestrogen‐secreting tumours which can lead to fatal myelotoxicity. The goal of this study was to describe the outcome of dogs with oestrogen‐induced bone marrow suppression secondary to Sertoli cell tumours in seven client‐owned dogs. Medical records from April 1, 2011 through April 1, 2021 were reviewed to identify dogs that underwent surgical management of a Sertoli cell tumour with documented bone marrow suppression. Overall, 5/7 dogs required transfusion of blood products peri‐operatively. Cases 1 and 6 received a transfusion of packed red blood cells (RBC) prior to surgery and case 5 required a transfusion of whole blood. Case 1 also required a transfusion of platelets before surgery. Post‐operatively, cases 1 and 2 received packed RBC's and case 6 received two transfusions of whole blood. Case 3 required transfusions of both fresh frozen plasma and platelets post‐operatively. All dogs survived to discharge and 6/7 dogs had documented improvement in haematopoietic values. Two dogs remained chronically thrombocytopenic. The median hospital stay was 4 days. One dog died within 4 weeks of surgery from worsening pancytopenia. Survival for greater than 1 year was documented in 4/7 dogs, and one dog was lost to follow‐up 4 months post‐operatively. One dog remained severely pancytopenic 4 weeks post‐operatively and received oral lithium treatment. Improvements in all blood cell lines were observed within the 4 weeks and resolution of pancytopenia within 6 weeks. Historically, the prognosis for dogs with bone marrow suppression secondary to Sertoli cell tumours was guarded to poor. This report documented improved outcomes for dogs that underwent surgery, including one dog that received lithium chloride as treatment for Sertoli cell tumour‐induced bone marrow suppression.
Impact of early life development on later onset chronic kidney disease and hypertension and the role of evolutionary trade‐offs
Authors: Luyckx Valerie A. , Chevalier Robert L. 
Published: December 2021

New Findings

What is the topic of this review?
In this report, we summarize the latest clinical evidence linking developmental programming in the kidney to later life blood pressure and kidney disease.

What advances does it highlight?
Population‐level studies now show convincingly that low birth weight, fetal growth restriction and preterm birth are associated with and have a synergistic impact on the risk of kidney disease in later life. A new approach also considers how evolutionary selection pressure might fail to select for long‐term robustness of kidney function.

AbstractThe global burden of kidney disease is high and rising. The risk of kidney disease among individuals is highly variable, in part related to genetic and environmental factors, but also likely to be modulated by developmental programming of the number of nephrons and kidney function in fetal life. The number of nephrons varies widely across the population and is lower among those who were born small or preterm. Population registry evidence clearly shows an association between these birth circumstances and later‐life risk of hypertension and kidney disease, not only for chronic kidney disease but also for acquired kidney disease, demonstrating an inherent susceptibility to kidney disease in these individuals. Gestational stressors impact kidney development, a process that is likely to be layered upon the evolutionary history of the kidney and how the organ has developed in response to selection pressure to support reproductive capacity in early adulthood, but not to withstand multiple stresses later in life. Reducing the global burden of kidney disease in future generations will require both individual‐ and population/environment‐level risks to be addressed.