bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 Detection Using Reverse Transcription Strand Invasion Based Amplification And A Portable Compact Size Instrument
Authors: Rosenstierne Maiken Worsøe , Joshi Shreya , Danielsen E. Thomas , Webb Helen , Luong Dac Mui , Bjerring Julie , Hindkær Julie , Jørgensen Lærke , Blauenfeldt Julie , Bojesen Ask , Holck Flemming , Lau Johnny , Bangsgaard Lars , Lind Jacob , Dragheim Mette , Jacobsen Mikkel , Elkær Robert , Clauwaert Steven , Christensen Kristina , Polacek Charlotta , Fomsgaard Anders , Ojalehto Tuomas , Tullila Antti , Brummer Mirko , Jensen Claus Antonio Juel , Jensen Frederikke Holm , Schneider Uffe Vest , Lisby Jan Gorm , Jørgensen Rikke Lind , Warthoe Thomas , Finding Ebbe , Warthoe Peter 
Published: July 2021

Abstract
Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal Reverse Transcription Strand Invasion Based Amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 minutes. The Egoo health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The performance of the Egoo Health System is comparable to the PCR instrument with an analytical sensitivity of 25 viral RNA copies per SARS-CoV-2 RT-SIBA reaction and a clinical sensitivity and specificity between 87.0-98.4% and 96.6-98.2% respectively.
Physicochemical Characterization of Cattle Dung Fibre Under the Hydrothermal Process
Authors: Fasake Vinayak , Kaur Perminder Jit , Dashora Kavya 
Published: July 2021

Abstract
Cattle dung fibre is a lignocellulosic material abundant and sustainable non-wood source of polymeric components, which can be converted into high added-value products. Hydrothermal treatment of the fibre obtained from cow dung was explored using four different temperatures (120, 140, 160 and 180 ◦C) and incubation times (0 and 120 minutes) at a fixed material to water ratio (1:10). The present study resulted in the highest yield of 94% (w/w) that gradually decreased with temperature and incubation time. The physicochemical analysis revealed that hydrothermal treatment resulted in high cellulose, low lignin, and ash content (51.6%, 30.93%, and 6.3%, respectively) at 160◦C for 2 hr incubation time and was appropriate for pulp and paper production. The SEM and X-ray crystallography indicates the treatment resulted in separated fibrils and a porous structure. Both FTIR studies and chemical characterisation techniques were used to optimize the temperature and duration of hydrothermal treatment. Overall, the study presents the first report on the extraction of fibres from cow dung and their hydrothermal treatment. In perspective, it is possible to achieve the properties required for its industrial-scale conversion to eco-friendly papers by heating the fibre under controlled conditions.
ADAMTS-13-Regulated Nrf2 Signaling Inhibits Ferroptosis to Ameliorate Cisplatin-Induced Acute Kidney Injury
Authors: Meng Xiaoyan , Huang Wenjing , Mo Weiwei , Shu Tingting , Yang Haoqiang , Ning Haibo 
Published: July 2021

Abstract
Background: ADAMTS-13 plays an important role in acute kidney injury (AKI), but the mechanism of cisplatin (CP) induced AKI remains unclear. Ferroptosis is increased in CP-induced AKI, and ADAMTS13 levels are associated with ferritin expression. In this article, we will explore the relationship between the three.Methods： After CP induction, mice were given 0.1 and 0.3nmol/kg ADAMTS-13, and then Scr and BUN were detected by the kits. The pathological changes of renal tissue were observed by staining with HE and PAS staining, and Western blot detected the expressions of KIM1 and NGAL in renal tissu. Perl's staining detected iron deposition in renal tissues, the kits detected iron levels, and western blot detected the expression of ferroptosis related proteins. Then the mechanism was further explored by adding ferroptosis inhibitors Ferrostatin 1 (Fer-1) and iron supplements Fe. The expression of Nrf2 pathway related proteins were detected by Western blot.Results: ADAMTS13 alleviated CP-induced ferroptosis in AKI mice with renal function impairment and tubular damage. Fer-1partially reversed CP-induced AKI, and Fe exacerbated this effect. ADAMTS13 alleviated CP-induced inflammatory response and oxidative stress in AKI mice, during which the Nrf2 signaling pathway was abnormal.Conclusion:ADAMTS-13-regulated Nrf2 signaling inhibits ferroptosis to ameliorate CP-induced AKI.
HPV E6/E7 mRNA In Situ Hybridization in Endocervical Adenocarcinoma: Implications for Prognosis and Diagnosis
Authors: Luo Rong-Zhen , Chen Shu-Lin , Li Mei , Li Yue , Yang Xia , Liu Li-Li 
Published: July 2021

Abstract
Background: Human papillomavirus (HPV) E6/E7 mRNA in situ hybridization (HPV E6/E7 RNAscope) appears to be a sensitive and specific technique in detecting transcriptionally active HPV. We aimed to examine the diagnostic utility of this technique in endocervical adenocarcinoma (ECA), to explore the prognostic factors for ECA patients and develop a clinically useful nomogram based on clinicopathological parameters to predict it.Methods: We retrospectively analyzed 200 patients with ECA who had undergone surgery at Sun Yat-sen University Cancer Center from 2010 and 2014. The diagnostic performance of HPV E6/E7 RNAscope were evaluated by receiver operating characteristic (ROC) curve. A prognostic nomogram model including HPV E6/E7 RNAscope was generated based on multivariate Cox regression analysis, then we compared the predictive accuracy of the prognostic model with FIGO staging and treatment using concordance index (C-index), time-dependent ROC (tdROC), and decision curve analysis (DCA).Results: The sensitivity and specificity of HPV E6/E7 RNAscope for distinguishing HPV-associated adenocarcinoma (HPVA) from non-HPV-associated adenocarcinoma (NHPVA) in the whole cohort were 75.8% and 80%, respectively. According the univariate analysis and multivariate logistic regression analysis, age, lymphovascular invasion (LVI), lymph node involvement (LNI), and HPV E6/E7 RNAscope were valuable predictive factors for OS. These parameters were incorporated into the nomogram model (nomogram A) compared with FIGO stage and treatment. The C-index of nomogram A for predicting OS was 0.825, which was significantly higher than FIGO stage (C-index = 0.653, p=0.002) and treatment (C-index = 0.578, p < 0.001).Conclusions: HPV E6/E7 RNAscope is highly specific for ECA, and the 4-variable nomogram showed more accurate prognostic outcomes in patients with ECA.
Harnessing the Protein Corona towards Surface Design for Nanoparticle Tumor Delivery
Authors: Ma Shijiao , Xu Junjie , He Jinxin , Li Shuli , Zheng Haolan , Pang Bo , Wen Ying , Fang Qiaojun , Liu Weiquan , Tian Jiesheng 
Published: July 2021

Abstract
Background

Nanoparticles (NPs) decorated with functional ligands (e.g., antibodies) are promising candidates for cancer diagnosis and treatment. However, many studies have shown that chemically coupled targeting moieties on NPs lose their targeting capability in biological milieu because they are shielded or covered by a "protein corona". It is highly desirable to increase the proportion of targeting moieties on the surface of NP-corona complex for purposes of cancer therapy.
Results

We designed and implemented an alternative engineering strategy for targeted drug delivery by linking a recombinant affibody (RA) scaffold to magnetosomes (BMPs). Pre-adsorption of BMP-RA with anti-HER2 humanized mAb trastuzumab (TZ) resulted in formation of BMP-RA-TZ complex. Linking of TZ to BMP via glutaraldehyde (GA) produced BMP-GA-TZ, which was used as control. Both engineered BMPs contained large amounts of TZ (~ 0.2 mg per mg BMP); however, TZs in BMP-RA-TZ were oriented in a consistent manner with HER2 binding site (Fab) toward the outside, whereas TZs in BMP-GA-TZ were oriented randomly. Degree of HER2 binding to BMP-RA-TZ was > 3x higher than that to BMP-GA-TZ. Incubation of BMP-RA-TZ and BMP-GA-TZ with normal human plasma or IgG-supplemented plasma resulted in alteration of surface proteins because of corona formation. GA-TZ-containing BMPs, in comparison with RA-TZ-containing BMPs, had larger hydrated radii and more surface proteins. The TZ-containing BMPs all could be targeted to and internalized in HER2-overexpressing breast cancer cell line SK-BR-3; however, their targeting efficiencies varied considerably: 50–75% for RA-TZ-containing BMPs, but only 9–19% for GA-TZ-containing BMPs. To simulate human body tumor environment, we incubated BMPs, plasma (100%), and cancer cells together. In this milieu, BMP-RA-TZ uptake efficiency of SK-BR-3 was nearly 80% (slightly lower than for direct interaction with BMP-RA-TZ), whereas BMP-GA-TZ uptake efficiency was < 17%.
Conclusion

Components of NP protein corona can be modified by linking RA scaffold to NP surface. Application of RA scaffold promoted an oriented arrangement of targeting ligands and reduced the shielding effect of corona proteins. This engineering strategy avoids reduction by corona formation of targeting capability in drug (NP) delivery.
Synthesis and Characterization of a Coagulating Agent From Plantain Peel Starch (Musa Paradisiaca), As Adjuvant in Water Treatment
Authors: Cortes-Perez Camilo , Pérez-Montalvo Leymer , Puello-Silva Jorge , Pasqualino Jorgelina , Lambis-Miranda Henry 
Published: July 2021

Abstract
Coagulation processes are widely used for water treatment, mainly with chemical coagulants. In this research, starch derived from a residue (plantain peel, Musa paradisiaca) was used as a starting point for a chemical modification. Through acetylation, its chemical structure was modified and characterized by Infrared Spectrophotometry, for its evaluation as a coadjuvant in coagulation operations to reduce the turbidity of raw water. Two experimental designs were developed to evaluate the incidence of modified starch as the main coagulant or in conjunction with a conventional coagulant (Al2(SO4)3), at different Al2(SO4)3 / acetylated starch ratios, in jar-test experiments. In the first experimental design, with the acetylated starch as the main coagulant, turbidity removal percentages reached 47.93% (average value, 41.18%). For the Al2(SO4)3 / acetylated starch coagulation process, 98.91% turbidity removal was reached in the second experimental design (average value, 97.16%). The incidence on turbidity in a jar-test of starch chemical substitution degree and the Al2(SO4)3 / Acetylated starch ratio was investigated using ANOVA analysis. There was a great incidence of the chemical substitution degree and the concentration of acetylated starch used, when modified starch was used as the main coagulant. For the second experimental design, the Al2(SO4)3 / Acetylated starch relationship had a greater incidence on the turbidity removal. Thus, modified starch obtained from plantain peel waste is a promising coadjuvant material for water coagulation processes.
Emergence of a putative novel SARS-CoV-2 P.X lineage harboring N234P and E471Q spike protein mutations in Southern Brazil
Authors: Silva Mariana Soares da , Gularte Juliana Schons , Demoliner Meriane , Hansen Alana Witt , Heldt Fágner Henrique , Filippi Micheli , Luckmann Cristiane Borba , Silveira Flávio , Pereira Vyctoria Malayhka de Abreu Góes , Vaucher Rodrigo de Almeida , Barboza Victor dos Santos , Giongo Janice Luehring , Rosa Raquel Borba , Rocha Evelise Tarouco da , Goulart Bruno Kilpp , Fernandes Fernanda dos Santos , Pinto Juliana Maciel , Bolzan Leandro Pergher , Duarte Marta Maria Medeiros Frescura , Weber Matheus Nunes , de Almeida Paula Rodrigues , Fleck Juliane Deise , Spilki Fernando Rosado 
Published: July 2021

Abstract

Novel SARS-CoV-2 lineages are constantly reported worldwide, raising concerns about transmissibility, virulence, immune response and vaccine/antigenic escape. Variants of concern (VOCs), as B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) and B.1.617.2 (Delta), caused epidemic outbreaks due their higher potential of transmissibility when compared with earlier waves of SARS-CoV-2 in 2019. B.1.1.28 lineage has been evolving in Brazil since February 2020 and originated P.1 (VOC), P.2 (VOI) and other P.Xs proposed as new variants. This lineage harbors specific defining mutations including two non-synonymous substitutions in the Spike (S) protein (D614G and V1176F). In this study, employing variant calling analysis on FASTQ reads and phylogenetic inference, we report a potentially new SARS-CoV-2 P.X variant. Variant calling mutational profile was investigated and presented additionally non-synonymous mutations when compared to B.1.1.28, including N234P and E471Q in S protein. Further studies are required to understand the spread of P.X variant and its potential effects on transmissibility and immune escape.
Nursing Care Case Report of Patient of COVID-19
Authors: Bilgin Saniye , Uysal Hilal 
Published: July 2021

Abstract
The functional health patterns model and nursing care process based on the nursing diagnosis of an individual with the diagnosis of COVID-19 were reviewed in this case report. An individual with the diagnosis of COVID-19 was evaluated in line with the functional health patterns (FHP) model, nursing diagnosis was made and a nursing care process was implemented in this case report. The data were collected by the researcher through observation and evaluation. N.B. was evaluated in line with the FHP nursing care model on 7 to 14 August 2020, and nursing procedures were implemented for these diagnoses and the results were evaluated.
Calculation of the Fine Structure Constant derived from Principle Theory.
Authors: Geilhaupt Manfred 
Published: July 2021

Abstract
Recently we have presented in a paper that the combination of two Principle Theories, General Relativity (GR) and Thermodynamics (TD), is able to derive the restmass (m) of an electron which (surprisingly) depends on the Sommerfeld Fine Structure Constant (FSC). In this paper we present a completed calculation of the FSC number (1/α=137.035999024(9)) from a GR+TD point of view.
The Healthy Female Microbiome Across Body Sites: Effect of Hormonal Contraceptives and the Menstrual Cycle
Authors: Krog Maria Christine , Hugerth Luisa W , Fransson Emma , Bashir Zahra , Andersen Anders Nyboe , Edfeldt Gabriella , Engstrand Lars , Nielsen Henriette Svarre , Schuppe-Koistinen Ina 
Published: July 2021

Abstract
Background: There is increasing evidence that the vaginal microbiome influences women’s health. V agina l dysbiosis with a low abundance of L actobacillus h as been connected to gynaecological and obstetric complications. However, the interplay between the vaginal microbiome and the microbiome in other body sites is not yet described. In addition, fluctuating endogenous sex hormones may exert an effect on microbiome composition and are markedly changed by hormonal contraception . This study includes a cohort of 160 healthy young Caucasian women using three different contraceptive regimens: non-hormonal methods, combined oral contraceptive (COC) or levonorgestrel intra-uterine system (LNG-IUS ) . The oral, vaginal, rectal and faecal microbiome s are characterized by shot gun sequencing during each phase of the menstrual cycle (menses, follicular and luteal phases). Results: The use of COC and LNG-IUS do not affect the microbiome composition or diversity . However , an increased diversity in the vaginal microbiome is observed during menses , followed by a subsequent expansion of Lactobacillus during the follicular and luteal phase s which correlate s with measured serum oestradiol levels (r = 0.11, p<0.001) . During menses 89 women (58%) ha ve a dysbiotic vaginal microbiome with less than 60% Lactobacillus spp . This decline s to 49 (32%) in the follicular phase (p = 1.3E-5) and 44 (29%) in the luteal phase (p = 4.6E-7) . During menses, bacterial richness and diversity in saliva reach its lowest while no difference is observed in the faecal microbiome . T he microbiome in different body sites is on average more similar within the same individual than between individuals , despite phase or hormonal treatment . Only the vagina present s a clear cluster structure with dominance of either Lactobacillus crispatus , Lactobacillus iners , Gardnerella vaginalis or Prevotella spp. Conclusions: The use of COC or LNG-IUS is not associated with changes of the healthy female microbiome except for increased stability in the vagina in LNG-IUS users . Body site is the main driver of the microbiome, including a clear difference between faecal and rectal samples. The menstrual cycle is a confounding factor for microbiome composition in both saliva and the vagina and should be considered when analysing the microbiome in women of reproductive age.
Elevated circASCC3 Suppresses Antitumor Immunity by Sponging miR-432-5p to Upregulate C5a in Non-small-cell Lung Cancer
Authors: Gao Jian , Zhang Ling-Xian , Ao Yong-Qiang , Jin Chun , Zhang Peng-Fei , Wang Hai-kun , Wang Shuai , Lin Miao , Jiang Jia-Hao , Ding Jian-Yong 
Published: July 2021

Abstract
Background: Tumor invasion and immune evasion are the main mechanisms underlying the progression of non-small-cell lung cancer (NSCLC). In addition, abnormally expressed circular RNAs (circRNAs) contribute to the malignant phenotype of NSCLC. Thus, further investigation of the mechanism of dysregulated circRNAs may provide new insight into the treatment of NSCLC.Methods: circRNA sequencing was used to explore the different expression profiles of circRNAs in 4 NSCLC tissues and paired normal tissues. Then, the expression of key circRNAs in NSCLC tissues and matched normal tissues was further evaluated via in situ hybridization and in cell lines using quantitative real-time polymerase chain reaction (qRT-PCR). Next, in vitro and in vivo models of NSCLC were employed to uncover the functions and mechanisms of key circRNAs in NSCLC progression and treatment.Results: circASCC3 (hsa_circ_0077495) was overexpressed in NSCLC tissues compared to paired normal tissues, and the upregulation of circASCC3 indicated a dismal prognosis in patients with NSCLC. Overexpressed circASCC3 enhanced the malignant phenotype of NSCLC cells in vitro and led to an immunosuppressive microenvironment in vivo. Mechanistically, circASCC3 sponged miR-432-5p to increase the expression of complement C5a, which induced the progression and dysfunctional immune status of NSCLC. Moreover, the combination of the C5aR inhibitor PMX-53 and anti-programmed cell death 1 (PD-1) antibody achieved synergistic effects in NSCLC models overexpressing circASCC3.Conclusion: These results uncover the contributions of circASCC3 to NSCLC progression and immunosuppression and provide a potential strategy for overcoming resistance to anti-PD-1 therapy.
Frequency of Mountain Waves Over Kanto Area Revealed by Imaging Observations of OH Airglow
Authors: Ishii Satoshi , Tomikawa Yoshihiro , Okuda Masahiro , Suzuki Hidehiko 
Published: July 2021

Abstract
Imaging observations of OH airglow were conducted at Meiji University, Japan (IN, mE), from May 2018 to December 2019. Mountainous areas, including Mt. Fuji, are located to the west of the imager, and westerly winds are dominant in the lower atmosphere throughout the year. Mountain waves (MWs) are generated on the leeward sides of mountains and occasionally propagate to the upper atmosphere. However, during the observation period (about 1 year and 8 months), only four possible MW events were identified. Based on previous reports, this incidence is considerably lower than expected. There are two possible reasons for the low incidence of MW events: (1) The frequency of MW excitation is small in the lower layers of the atmosphere, and/or (2) MWs do not propagate easily to the upper mesosphere due to background wind conditions. This study verified the likelihood of the former case. Under over-mountain airflow conditions, wavy clouds are often generated on the leeward side. Since over-mountain airflow is essential for the excitation of MWs, the frequency of wavy clouds in the lower atmosphere can be regarded as a measure of the occurrence of MWs. The frequency and spatial distribution of MWs around Japan were investigated by detecting the wavy clouds from color images taken by the Himawari-8 geostationary meteorological satellite (GSM-8) for one year in 2018. The wavy clouds were detected on more than 70 days a year around the Tohoku region, but just 20 days a year around Mt. Fuji. This suggests that few MWs are generated around Mt. Fuji. The differences between these two regions were examined focusing on the relationship between the local topography and dominant horizontal wind fields in the lower atmosphere. Specifically, the findings showed that the angle between the dominant horizontal wind direction and the orientation of the mountain ridge is a good proxy of the occurrence of wavy clouds, i.e., excitation of MWs in mountainous areas. We have also applied this proxy to topography in other areas of the world to investigate areas where MWs would be occurring frequently. Finally, we discuss the likelihood of "MW hotspots" at various spatial scales in the world.
Is Diabetic Ketoacidosis A Good Predictor Of 5-Year Metabolic Control in Children with Newly Diagnosed Type 1 Diabetes?
Authors: Kowalczyk Emilia , Stypułkowska Aneta , Majewska Barbara , Domaradzka Małgorzata , Hoffmann Aleksandra , Buła Paulina , Szypowska Agnieszka 
Published: July 2021

Abstract
Purpose: The study aimed to evaluate whether the presence of DKA at diabetes diagnosis was associated with poorer metabolic control during a 5-year follow-up.Methods: The study included children treated due to newly diagnosed T1D complicated with DKA between 2010-2014 with a complete 5-year follow-up. In every case we performed individual matching for age, gender and BMI with a person without DKA (nDKA) on recognition. We collected data regarding treatment modality, HbA1C, total daily insulin dose, basal insulin and BMI-SDS.Results: 85 children at the median age of 7.93 years had DKA at diabetes diagnosis. The median pH was 7.19.Continuous subcutaneous insulin infusion (CSII) was used in 87% of participants in each group. No differences in HbA1C level (7,3%vs7,2%;p=.413) were noted after 5 years of disease duration. The severity of ketoacidosis exerted no significant effect on HbA1C. The method of insulin delivery at baseline was significantly associated with HbA1C levels after 5 years of observation, βCSII=-1.46,95%CI[-2.01 to -0.92],p<.001.Conclusions: The presence of DKA at diabetes diagnosis is not associated with deteriorated long-term metabolic control in children using modern technologies. The early implementation of CSII into diabetes treatment may change the effect of DKA and lead to a long-term HbA1C improvement.