214 SIRPα-deficient macrophages activate polyclonal tissue-resident cytotoxic CD8 T cells to eliminate irradiation-refractory non-small cell lung carcinoma
Authors: Bian Zhen , Shi Lei , Kidder Koby , Liu Yuan 
Published: November 2021

BackgroundRadiotherapy (RT) is a mainstay treatment for cancer and may not only eliminate irradiated tumors but also induce cytotoxic CD8 T cell immunity to regress abscopal metastases. However, even when combining RT with immunomodulatory agents, adequate immune-mediated tumor elimination is limited, especially when tumors are advanced and highly immunosuppressive. Thus, revealing the mechanisms by which tumors resist RT and resolving methods to enhance RT-induced immunity are needed. SIRPα is a myeloid-expressed receptor that inhibits macrophages from phagocytosing self-cells by interacting with its ligand CD47. Cancers often increase their CD47 to suppress macrophages. Although merely depleting SIRPα-mediated inhibition (SIRPα-deficient) does not elicit phagocytosis, activating SIRPα-deficient macrophages with certain proinflammatory cytokines or TLR agonists enables them to phagocytose cancer cells. Given that macrophages are abundant in solid tumors and often contribute to RT-resistance, we postulated that RT-induced damage in the tumor microenvironment (TME) would stimulate TLRs on SIRPα-deficient macrophages to then phagocytose cancer cells and better control RT-resistant tumors.MethodsTumors were established by engrafting syngeneic non-small cell lung carcinoma (LLC) into wild-type (WT) or SIRPα-deficient C57BL6 mice. Tumors were allowed to progress to various stages, then mice were treated with tumor-directed RT (4–15Gy). In some cases, RT was combined with intravenous or intratumoral adoptive transfer of macrophages. To compare the therapeutic efficacy of RT plus macrophage transfer, head-to-head studies were conducted using anti-CD47 antibodies or SIRPα-Fc fusion-proteins with cancer-specific antibodies and RT. Flow cytometry, Nanostring RNA sequencing, and several cell function assays were used to analyze responses to treatments.ResultsGlobal knockout of SIRPα in mice sensitizes their otherwise RT-resistant tumors to low-dose RT and achieves up to 100% complete response and 100% overall survival. SIRPα-deficient macrophages are essential to the therapeutic response, as depleting them abrogated efficacy while transferring them recapitulated this efficacy in WT mice. SIRPα-deficient macrophages not only eliminate tumors by phagocytosing cancer cells but more so by conducting in situ antigen presentation to CD8 T cells that are highly cancer-specific and cytotoxic. In addition, RT synergizes with activated SIRPα-deficient macrophages to reprogram the TME into a pro-inflammatory niche infiltrated by cytotoxic NK cells and neutrophils but comprising few CD4 T regulatory cells (Tregs) and myeloid-derived suppressor cells (MDSCs).ConclusionsThe results demonstrate that SIRPα predominantly underlies tumor resistance to RT and provide proof-of-principle for SIRPα-deficient macrophage-based therapies to cure lung cancer, including those at advanced stages with poor immunogenicity and metastases.AcknowledgementsThe authors thank the Georgia State University Animal Resources Program for assisting many experiments; This work was supported, in part, by grants from National Institutes of Health (CA241271 and AI106839), a Georgia Research Alliance (GRA) Venture Development grant, a Biolocity Innovation & Commercialization grant, a Careers in Immunology fellowship from American Association of Immunologist (Z.B.), a Molecular Basis of Disease fellowship from Georgia State University (K.K.) and an Ahmed T. Abdelaal Molecular Genetics and Biotechnology fellowship from Georgia State University (K.K.).Ethics ApprovalAll experiments using animals and procedures of animal care and handling were carried out following protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Georgia State University.