bluuebunny/crossref_metadata_2025_split huggingface dataset:
Explant Cultures From Prostate Needle Biopsies as A Model to Obtain Highly Enriched Populations of Benign and Carcinoma-Associated Fibroblasts
Authors: Cerda-Infante Javier , Sánchez Marianela , Rojas Pablo A. , Corvalán Alejandro H. , Méndez Gonzalo P. , Nervi Bruno , Smith Gary J. , Francisco Ignacio F. San , Godoy Alejandro S. , Montecinos Viviana P. 
Published: March 2021

Abstract
Prostate cancer (PCa) is a multifocal neoplasm in which different cancer foci can be found within a single organ. This feature represents a technical difficulty when obtaining highly-enriched primary cultures of human prostate stroma cells (HPSCs), since their isolation involves enzymatic digestion of large pieces of tissue, resulting in an unknown proportion of benign- (BAF) and carcinoma-associated fibroblasts (CAF). Here we report an efficient tissue explant methodology to obtain highly-enriched populations of BAFs or CAFs from benign and malignant human prostate specimens, including needle biopsies. Also, we investigated whether different histological/pathological origins of the prostate tissue determine the activation of distinct transcription programs in HPSCs. Analysis of gene expression profiles revealed that CAFs exhibited a unique transcriptional program characterized by up-regulation of genes involved in proliferation, migration and cell adhesion. CAFs, but not BAFs, expressed typical activation markers such as α-smooth muscle actin, tenascin C and collagen 1 and stimulated tumor growth of PCa cells in vivo. Our study indicated that gene expression profile of HPSCs was determined mostly by the zone rather than the pathological state of the prostate tissue and that tissue explants from needle biopsies provide a valuable tool to obtain highly enriched primary cultures of HPSCs.
Tyramide Signal Amplification coupled with multiple immunolabeling and RNAscope in situ hybridization in formaldehyde-fixed paraffin-embedded human fetal brain.
Authors: Alzu'bi Ayman , Sankar Niveditha , Clowry Gavin 
Published: March 2021

Abstract
Several strategies have been recently introduced to improve the practicality of multiple immunolabelling and RNA in situ hybridization methods. We present a modified hybrid protocol of recently described complex detection strategies: (1) elution of antibodies prior to second round of staining (2) use of integrated polymers of HRP with secondary antibodies, and (3) tyramide signal amplification of multiple immunofluorescence labelling, to achieve a high sensitivity sequential multiple labeling using antibodies from the same species. A modified protocol of the novel RNAscope in situ hybridization method, including coupling with immunofluorescence on sections of early human fetal brain, has also been developed. These two techniques, when properly optimized, were highly compatible with routine formaldehyde-fixed paraffin-embedded tissue that preserves the best morphological characteristics of delicate fetal brain samples, allowing high power signal amplification for detection of protein and mRNA of genes that are sparsely expressed in the human fetal telencephalon.
Descriptive Cross-Sectional Study on Acanthamoeba - Associated Pseudomonas Species at Kenyatta National Hospital Intensive Care Unit
Authors: Macharia Zipporah W. , Mureithi Marianne W. , Mburugu Magdalene W. , Gitau Wilfred , Masika Moses M. , Njaanake Kariuki 
Published: March 2021

Abstract
Background

Free-living amoeba (FLA) such as Acanthamoeba spp. are ubiquitous protozoa that graze on microorganisms such as bacteria, viruses and algae. However, amoebic resistant microorganisms (ARM) such as Pseudomonas spp. evade being killed by amoeba thus multiplying within the free-living amoeba. Free-living amoeba may therefore enhance transmission of Pseudomonas spp. across hospital environments thus contributing to the burden of nosocomial infections and spread of antimicrobial resistance. In Kenya, nosocomial Pseudomonas spp. infections present a major health challenge but the role of free-living amoeba such as Acanthamoeba spp. in transmission of these infections has so far not been assessed. This study aimed at the isolation of Acanthamoeba spp. from various surfaces and equipment and molecular detection of the Acanthamoeba-associated Pseudomonas spp.
Methods

This was a descriptive cross-sectional study in which 153 swabs in duplicate (306) were collected from selected surfaces and equipment. Acanthamoeba spp. cultures were performed on one batch of 153 swabs and bacterial cultures on the other batch of 153 swabs. Polymerase chain reaction (PCR) was performed to detect Pseudomonas sp. genomic deoxyribonucleic acid (DNA) within Acanthamoeba spp. isolates.
Results

The proportion of swabs that had Acanthamoeba spp. isolates was 93.5% (n = 153) while that of bacterial isolates was 82.4% (n = 153). Of 168 bacterial isolates obtained, 10.7% was Pseudomonas spp. Acanthamoeba spp. subcultures were done on 62.7% (n = 153) primary Acanthamoeba spp. isolates. Only 22.9% (n = 96) of the Acanthamoeba spp. subcultures were successful. Pseudomonas sp. genomic DNA was detected in 45.5% (n = 22) of all positive Acanthamoeba spp. subcultures using PCR.
Conclusion

Pseudomonas sp., an ARM, exists within Acanthamoeba spp. on various surfaces and equipment at KNH ICU. This suggests a possible role of Acanthamoeba spp. in Acanthamoeba- ARM associated nosocomial infections transmission and in spread of antimicrobial resistant genes across microorganisms in hospitals.
Evidence-based recommender system and experimental validation for high-entropy alloys
Authors: Ha Minh-Quyet , Nguyen Nguyen-Duong , Nguyen Viet-Cuong , Nagata Takahiro , Chikyow Toyohiro , Kino Hiori , Miyake Takashi , Denœux Thierry , Huynh Van-Nam , Dam Hieu-Chi 
Published: March 2021

Abstract
We present a data-driven approach to explore high-entropy alloys (HEAs). To overcome the challenges with numerous element-combination candidates, selecting appropriate descriptors, and the limitations and biased of existing data, we apply the evidence theory to develop a descriptor-free evidence-based recommender system (ERS) for recommending HEAs. The proposed system measures the similarities between element combinations and utilizes it to recommend potential HEAs. To evaluate the ERS, we compare its HEA-recommendation capability with those of matrix-factorization- and supervised-learning-based recommender systems on four widely known data sets, including binary and ternary alloys. The results of experiments using k-fold cross-validation on the data sets show that the ERS outperforms all competitors. Furthermore, the ERS shows excellent extrapolation capabilities in experiments of recommending quaternary and quinary HEAs. We experimentally validate the most strongly recommended Fe-Co-based magnetic HEA, viz. FeCoMnNi, and confirm that it shows a body-centered cubic structure and is stable at high temperatures.
Quantitative proteomic and lipidomics analyses of high oil content GmDGAT1-2 transgenic soybean, illustrates the regulatory mechanism of lipoxygenase and oleosin
Authors: Xu Yang , Yan Fan , Liu Yajing , Wang Ying , Gao Han , Zhao Shihui , Wang Qingyu , Li Jingwen 
Published: March 2021

Abstract
The main goal of soybean breeding is to increase the oil content. Diacylglycerol acyltransferase (DGAT) is a key rate-limiting enzyme in fatty acid metabolism, and may regulate oil content. Herein, 10 GmDGAT genes were isolated from soybean and transferred into wild-type (WT) Arabidopsis. The total fatty acid was 1.2 times higher in T3 GmDGAT1-2 transgenic Arabidopsis seeds than in WT. Therefore, GmDGAT1-2 was transferred into WT soybean (JACK), and four T3 transgenic soybean lines were obtained. The results of High performance gas chromatography and Soxhlet extractor showed that, compared with those of JACK, oleic acid (18:1), and total fatty acid levels in transgenic soybean plants were much higher, but linoleic acid (18:2) was lower than WT. Palmitic acid (16:0), stearic acid (18:0), and linolenic acid (18:3) were not significantly different. For mechanistic studies, 436 differentially expressed proteins (DEPs) and 180 differentially expressed metabolites (DEMs) were identified between WT (JACK) and transgenic soybean pods using proteomic and lipidomics analyses. Four lipoxygenase proteins were down-regulated in linoleic acid metabolism while four oleosin proteins were up-regulated in the final oil formation. The results showed an increase in the total fatty acid and 18:1 composition, and a decrease in the 18:2 composition of fatty acid. Our study brings new insights into soybean genetic transformation and the deep study of molecular mechanism that changes the total fatty acid, 18:1, and 18:2 compositions in GmDGAT1-2 transgenic soybean.
Parametrized Quantum Circuits of Synonymous Sentences in Quantum Natural Language Processing
Authors: Salari Vahid , Abbaszadeh Mina , Mousavi Seyyed Shahin 
Published: March 2021

Abstract
In this paper we develop a compositional vector-based semantics of positive transitive sentences in quantum natural language processing for a non-English language, i.e. Persian, to compare the parametrised quantum circuits of two synonymous sentences in two languages, English and Persian. By considering grammar+meaning of a transitive sentence, we translateDisCoCat diagram via ZX-calculus into quantum circuit form. Also, we use a bigraph method to rewrite DisCoCat diagram and turn into quantum circuit in the semantic side.
Age-dependent seroprevalence of SARS-CoV-2 antibodies in school-aged children from areas with low and high community transmission
Authors: Boey Lise , Roelants Mathieu , Merckx Joanna , Hens Niel , Desombere Isabelle , Duysburgh Els , Vandermeulen Corinne 
Published: March 2021

Abstract
BackgroundIt is not yet clear to what extent SARS-CoV-2 infection rates in children reflect community transmission, nor whether infection rates differ between primary schoolchildren and young teenagers.MethodsA cross-sectional serosurvey compared the SARS-CoV2 attack-rate in a sample of 362 children recruited from September 21 to October 6, 2020 in primary (ages 6-12) or lower secondary school (ages 12-15) in a municipality with low community transmission (Pelt) to a municipality with high community transmission (Alken) in Belgium. Children were equally distributed over grades and regions. Blood samples were tested for the presence of antibodies to SARS-CoV-2 with an enzyme-linked immunosorbent assay. ResultsWe found anti-SARS-CoV-2 antibodies in 4.4% of children in the low transmission region and in 14.4% of children in the high transmission region. None of the primary schoolchildren were seropositive in the low transmission region, whereas the seroprevalence among primary and secondary schoolchildren did not differ significantly in the high transmission region. None of the seropositive children suffered from severe disease. Children who were in contact with a confirmed case (RR: 3.8; 95%CI: 1.7 – 8.3), who participated in extracurricular activities (RR: 5.6; 95%CI: 1.2 – 25.3) or whose caregiver is a healthcare worker who had contact with COVID-19 patients (RR: 2.2; 95%CI: 1.0 – 4.6), were at higher risk of seropositivity.ConclusionIf SARS-CoV2 circulation in the community is high, this will be reflected in the pediatric population with similar infection rates in children aged 6-12 years and 12-15 years.
Bifurcation Analysis of Rotor/Bearing System using Third Order Journal Bearings Stiffness and Damping Coefficients
Authors: Elsayed Tamer , Sayed Hussein 
Published: March 2021

Abstract
Journal bearings have many applications in industry due to its high load carrying capacity. In addition proper design of journal bearings enables safe operation at very high speeds. However, they are susceptible to oil whirl instability which may cause bearing failure. The fluid film pressure distribution inside the journal bearing is described by Reynolds equation. Many studies had been done to approximate the bearing performance using first order bearing coefficients. Although this analysis is stable for evaluating the threshold speed but it is insensitive to limit cycles above the threshold speed. Mush literature show that above the threshold speed, subcritical or supercritical bifurcations may be observed. Therefore, the aim of the present paper is to evaluate the third order bearing coefficients for a finite length journal bearing using finite perturbation method. The values of these coefficients are evaluated using infinitesimal perturbation analysis. These values are used to investigate the bifurcation stability of flexible Jeffcott rotor supported by two symmetric journal bearings. The effect of rotor stiffness ratio on the bifurcation stability of the system is investigated. The results of this work show that the third order parameters can be used to evaluate the type of bifurcation above the threshold speed.
Porous Wood Decorated with Gold Nanoparticles as Flow-through Membrane Reactor for Catalytic Hydrogenation of Methylene Blue and 4-Nitrophenol
Authors: Yu Yuanyuan , Zhang Qingtong , Chi Mingchao , Jiang Hongrui , Liu Xi , Wang Shuangfei , Min Douyong 
Published: March 2021

Abstract
In this study, gold nanoparticles (Au NPs) were decorated into Paulownia Sieb. et Zucc. chip. Lignin, as one main component of wood, contains the reducing groups e.g. hydroxyl, carbonyl and aldehyde groups. Under sunlight irradiation, Au (III) diffused into wood was in situ reduced by lignin to form gold nanoparticles. Therefore, the Au NPs/Wood was successfully fabricated by this facile and green procedure. Meanwhile, the three-dimensional interpenetrating network of wood prevented the aggregation of Au NPs which retained its catalytic activity. Methylene blue and 4-nitrophenol were adopted as model organic contaminants to evaluate the catalytic hydrogenation ability of the Au NPs/Wood. The analyses of XRD, SEM, ICP-MS and XPS indicated that Au NPs were successfully immobilized in wood chip. The degradation results revealed that the Au NPs/Wood has excellent catalytic activity for methylene blue and 4-nitrophenol hydrogenation under batchwise and continuous flow process. Meanwhile, the Au NPs/Wood also exhibited excellent recyclability. The hydrogenation efficiency of MB and 4-NP still reaches more than 90% after 8 cycles. This study provides a new solution for green and low-cost fabrication of Au NPs/Wood which has broad application prospects in wastewater treatment.
Housing and Mental Health during Outbreak of COVID-19
Authors: Akbari Paria , Yazdanfar Seyed-Abbas , Hosseini Seyed-Bagher , Norouzian-Maleki Saeid 
Published: March 2021

Abstract
Background: Recently, a novel coronavirus (COVID-19) has caused global health concerns. Due to the outbreak of COVID-19, house quarantine was considered to prevent the outbreak of the disease and ensure physical health, but it may cause serious mental health problems. The present study aims to assess housing satisfaction, housing preference of residents, and their mental health in house quarantine of COVID-19 considering housing type, spaces, environmental factors, and function and activities. Methods: Quantitative data were gathered through administering online questionnaires. In April 2020, 421 valid responses were gathered from people who were living in Tehran. Then the collected data were analyzed using SPSS. Results: According to the result, environmental factors have a higher mean than spaces and functions and activities throughout housing preference. Satisfaction with all parameters affects improving mental health. The kitchen, view quality, exercise, and cultivation and maintenance of plants have the greatest impact on improving mental health among house spaces, environmental factors, activities, and functions, respectively. The mental health of people living in private houses is better than residents of low-rise and high-rise housing.Conclusions: Residents’ opinions about the houses showed that there are differences between the current situation of the house and the preferences of the people during the house quarantine period, which has been effective in the mental health of the residents in this period. Consideration of parameters in housing design by architects and home planners can improve people's mental health during special and critical situations such as house quarantine due to the spread of epidemics.
In-Situ Passive Infrared Thermography Application for the Assessment of Localized Mechanical Properties of&nbsp;TIG-Welded Inconel 625 Alloys
Authors: Rawashdeh Nathir A , Atieh Anas M , Bani-Mostafa Diala 
Published: March 2021

Abstract
Traditional evaluation of weld joint mechanical properties by destructive testing shows the limitations of these evaluation methods, including equipment limitations, high materials/testing cost, and challenging repeatability. This work presents an experimental approach for utilizing passive infrared thermography and tensile testing to evaluate the correlation between thermal and localized TIG-welded Inconel 625 joints' mechanical properties. The results show that different areas of interest behave differently during the tensile test. The dog-bone shaped TIG-welded samples were divided into five regions of interest on both sides of the weldment, of which two are repetitive on both sides of the sample representing Inconel 625 base metal alloy, heat-affected zones, and weld beads. The temperature change rate in these three regions varied from 0.17oC/min to 0.67oC/min as the tensile test progresses. The fractography analysis showed that the failure occurred within the weld beads, even though the highest temperature was observed in the heat-affected zones, suggesting higher strength in the heat-affected zones. This work's suggested evaluation method provides a powerful industrial and experimental tool to predict weld failure based on temperature changes during static loading.
A Numerical Study of Chemical Reaction in a Nanofluid Flow Due to Rotating Disk in the Presence of Magnetic Field
Authors: Ramzan Muhammad , Kumam Poom , Nisar Kottakkaran Sooppy , Khan Ilyas , Jamshed Wasim 
Published: March 2021

Abstract
In this paper, a numerical study of MHD steady flow due to the rotating disk with chemical reaction was explored. Effect of different parameters such as Schmidt number, chemical reaction parameter, Prandtl number, Suction parameter, heat absorption/generation parameter, Nano-particle concentration, Reynold number, Magnetic parameter, skin friction, shear stress, temperature distribution, Nusselt number, mass transfer rate, radial velocity, axial velocity, and tangential velocity was analyzed and discussed. For the simplification of non-linear partial differential equations (PDEs) into the nonlinear ordinary differential equation (ODEs), the method of Similarity transformation was employed, and the resulting partial differential equation was solved by using finite difference method through MATLAB programming. This work's remarkable finding is that with the expansion of nanoparticle concentration radial velocity, tangential velocity and temperature of the fluid was enhanced but reverse reaction for axial velocity. Furthermore, the present results are found to be in excellent agreement with previously published work.
Investigation on the Microstructures and Mechanical Properties of Friction Stir Processed 2A14 Aluminum Alloy Fabricated by Different Initial Precipitation States
Authors: Wang Jian , Yang Ke , Zhou Dongshuai , Xie Li , Lu Yalin , Li Xingcheng 
Published: March 2021

Abstract
6 mm thick 2A14 Al alloy plates were friction stir processed with different initial precipitation states of as-cast, homogenization, rolling and T6. The results indicated that FSP can dramatically reduce the grain and particles size and promote the formation of fine recrystallized grains with random orientation in the stirred zone (SZ). No significant influences of the initial precipitation states of base metal (BM) on the grain size, grain boundary characteristic, texture component and texture intensity of SZ were perceived. Rather, the initial precipitation states can evidently affect the morphology and distribution of precipitates and dislocations. The dominant C and A1* texture components developed in the SZ are correlated with the shear deformation and dynamic recrystallization. And the weakened texture intensity created in the SZ after FSP also signifying that discontinuous dynamic recrystallization might be involved. Compared with the corresponding BM, the SZ fabricated by the BM under as-cast and homogenized states were strengthened arises from the obvious refined grains, uniform dispersed particles and elimination of casting defects caused by FSP, while the softening of SZ were observed for the BM under rolled and T6 states, which are mainly dependent on the reduction of dislocation density and dissolution/coarsening of fine precipitates during FSP.
Impact of Sleeve Gastrectomy on Renal Function in Patients With Morbid Obesity: A One-Year Prospective Cohort Study
Authors: Sanchez Delphine , Lebrun Amandine , Somda Sosthene , Lainas Panagiotis , Tranchart Hadrien , Lamouri Karima , Prevot Sophie , Njike-Nakseu Micheline , Gaillard Martin , Zaidan Mohamad , Balian Axel , Dagher Ibrahim , Naveau Sylvie , Perlemuter Gabriel , Voican Cosmin Sebastian 
Published: March 2021

Abstract
Background. Obesity is an independent risk factor for renal injury. A more favorable metabolic environment following weight loss may theoretically lead to improved renal function. We aimed to evaluate the evolution of renal function one year after sleeve gastrectomy in a large prospective cohort of patients with morbid obesity and assess the influence of fat-free mass (FFM) changes.Patients and Methods. We prospectively included 563 severely obese patients admitted for sleeve gastrectomy. Patients were systematically evaluated one year after surgery. The estimated glomerular filtration rate (eGFR) was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation. The FFM was estimated by analyzing computerized tomography (CT) scan sections from CT systematically performed two days and one year after sleeve gastrectomy to detect surgery complications.Results. The mean age was 41.2±0.52 years. The mean body mass index was 43.5±0.3 kg/m2 and 20.4, 30.5, and 30.7% of the included patients had type 2 diabetes, hypertension, and dyslipidemia, respectively. One hundred and fifteen patients were lost to follow-up at one-year post-surgery. The eGFR was significantly lower one year after sleeve gastrectomy than before surgery (87.8±0.9 versus 86.1±0.9, p<0.01). There was no difference in terms of post-surgery FFM loss between patients with an improved eGFR and those without (6.7±0.3 kg versus 6.76±0.5 kg, p=0.9). Furthermore, post-surgery changes in the eGFR did not correlate with the amount of FFM loss (r=0.1, p=0.18).Conclusion. Renal function assessed by eGFR is significantly improved at one-year post-sleeve gastrectomy, independent of changes in skeletal muscle mass.