bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic Analysis of 1-Dof and 2-Dof Nonlinear Energy Sink With Geometrically Nonlinear Damping and Combined Stiffness
Authors: Zhang Yunfa , Kong Xianren , Yue Chengfei , Xiong Huai 
Published: April 2021

Abstract
Nonlinear energy sink (NES) refers to a typical passive vibration device connected to linear or weakly nonlinear structures for vibration absorption and mitigation. This study investigates the dynamics of 1-dof and 2-dof NES with nonlinear damping and combined stiffness connected to a linear oscillator. For the system of 1-dof NES, a truncation damping and failure frequency are revealed through bifurcation analysis using the complex variable averaging method. The frequency detuning interval for the existence of the strongly modulated response (SMR) is also reported . For the system of 2-dof NES, it is reported in a similar bifurcation analysis that the mass distribution between NES affects the maximum value of saddle-node bifurcation. To obtain the periodic solution of the 2-dof NES system with the consideration of frequency detuning, the incremental harmonic balance method (IHB) and Floquet theory are employed. The corresponding response regime is obtained by Poincare mapping, it shows that the responses of the linear oscillator and 2-dof NES are not always consistent, and 2-dof NES can generate extra SMR than 1-dof NES. Finally, the vibration suppression effect of the proposed NES with nonlinear damping and combined stiffness is analyzed and verified by the energy spectrum, and it also shows that the 2-dof NES system demonstrates better performance.
Early detection of emergency events from social media: A new text clustering approach
Authors: Huang Lida , Shi Panpan , Zhu Haichao , Chen Tao 
Published: April 2021

Abstract
Emergency events need early detection, quick response, and accuracy recover. In the era of big data, social media users can be seen as social sensors to monitor real time emergency events. This paper proposed an integrated approach to early detect all the four kinds of emergency events including natural disasters, man-made accidents, public health events and social security events. First, the BERT-Att-BiLSTM model is used to detect emergency related posts from the massive and irrelevant data. Then, the 3W attribute information (What, Where and When) of the emergency event is extracted. With the 3W attribute information, we create an unsupervised dynamical event clustering algorithm based on text-similarity and combine it with the supervised logistical regression model to cluster posts into different events. The experiments on Sina Weibo data demonstrate the superiority of the proposed framework. Case studies on some real emergency events show the proposed framework has good performance and high timeliness. Practical applications of the framework have also been discussed, following by some future directions for improvement.
Effect of Cold Exposure and Capsaicin on the Expression of Histone Acetylation and Toll-like Receptors in 1,2-dimethylhydrazine-induced Colon Carcinogenesis
Authors: Qin Jingchun , Li Huixuan , Yu Weitao , Wei Li , Wen Bin 
Published: April 2021

Abstract
Previous studies have indicated that capsaicin-rich diet and cold weather have showed strong association with tumor incidence. Thus, we investigated the effects of capsaicin and cold exposure in 1,2-dimethylhydrazine-induced colorectal cancer as well as the mechanisms underlying capsaicin and cold induced CRC. Rats were randomly divided into four groups and received cold still water and capsaicin via intragastric gavage until the end of the experiment. The rat’s body weight, thymus weight, and food intakes were assessed. Global levels of histone H3K9, H3K18, H3K27, H4K16 acetylation and, histone deacetylase (HDACs) in colon mucosa were assessed by western blot. Expression levels of Toll-like receptors 2 (TLR2) and Toll-like receptors 4 (TLR4) were measured by western-blot and reverse-transcriptase quantitative polymerase chain reaction (qPCR). We found that cold and low-does capsaicin increased tumor numbers and multiplicity, although there were no differences in tumor incidence. Additionally, rat exposure of cold water and capsaicin display further higher levels of histone H3 lysine 9 (H3K9AC), histone H3 lysine 18 (H3K18AC), histone H3 lysine 27 (H3K27AC) and HDACs compared with the DMH and normal rats. In contrast, a considerable decrease of histone H4 lysine 16 (H4K16AC) was detected in the colon mucosa. Cold and low-dose capsaicin exposure group were also increased TLR2 and TLR4 proteins levels and mRNA levels. These results suggest that chronic cold exposure and capsaicin at a low-does intervention exacerbate ectopic expression of global histone acetylation and TLR level, which are crucial mechanisms responsible for the progression of colorectal cancer in rat.
Simultaneous Removal of Iron and Hexavalent Chromium Via Slow Sand Filter Inoculated With Thiobacillus ferrooxidans and Waste management
Authors: Mian Abrar Hussain 
Published: April 2021

Abstract
Many places have common physical, biological, and chemical hazards and heavy metals contamination in drinking water is very important to study especially near industrial areas and mining sources. Application of microbes along with other physicochemical techniques can prove to be the suitable remediation technology for the removal of metals from water. This study aimed to remove iron and hexavalent chromium from water using slow sand filters inoculated with iron-oxidizing bacteria Acidothiobacillus ferrooxidans. An experiment was performed for 37 days under controlled conditions, a water sample was prepared synthetically and another sample was collected from water filtration plant from Abbottabad city in KP Province- Pakistan and influents and effluents were tested for different parameters like pH, turbidity, hardness, total plate count, total dissolved solids, electrical conductivity and analysis of iron and hexavalent chromium in water. Acidothiobacillus ferrooxidans culture was isolated from soil using the differential iron media i.e. 9K medium and was inoculated into sand filters. At the end of the experiment, the removal efficiency of ferrous iron was about 52% and that of hexavalent chromium was 60.2%. In simultaneous treatment, it was observed that removal efficiency of iron was about 68% while that of hexavalent chromium was about 93% suggesting that slow sand filters were more effective for the removal of hexavalent chromium than iron. Hence, the designed slow sand filters were able to reduce the concentration of hexavalent chromium more efficiently than ferrous iron. And it was observed that slow sand filters can effectively remove ferrous iron and hexavalent chromium from water if they are inoculated with respective bacteria. Key,words,. Hexavalent Chromium, Iron Oxidizing, Sand Filters, Acidothiobacillus ferrooxidans, waste management.
Low-dose Exposure to Black Carbon and Cadmium Significantly Induce Lung Injury by Promoting Cellular Apoptosis
Authors: Wang Lingjuan , Bao Shanyu , Liu Xiaolong , Wang Fan , Zhang Jinwei , Dang Peng yu , Huang Wen , Li Bin , Lin Yi 
Published: April 2021

Abstract
PMs has adverse biological effects on major living organs in the body, including lungs. The complex composition of PM2.5, including carbon black and heavy metals, cause toxic effects to the lung. The synergistic effects of CB and Cd were less investigated in previous study. In our research, we deciphered the combination of CBs and Cd enhanced the cytotoxicity in BEAS-2B cells in vitro and in vivo. In the molecular level, the intracellular level of Cd increased, as a result of the cell membrane damage, causing high expression of MT-1 in BEAS-2B cells. Moreover, the expression of BAX increased, and the expression of BCL-2 decreased. Collectively, our data suggests the apoptosis effect of synergistic effects of CB and Cd exposure.
The MiR-193a-5p/NCX2/AKT Axis Promotes Invasion and Metastasis of Osteosarcoma
Authors: Chen Ruiqi , Ning Yichong , Zeng Guirong , Zhou Hao , Tu Chao , Li Shuangqing , Qi Lin , Li Zhihong , Zhou Jianlin 
Published: April 2021

Abstract
Background: MiR-193a-5p has been observed to have oncogenic or tumor-suppressive function in different kinds of cancers. Na+/Ca2+ exchangers (NCX1, NCX2 and NCX3) normally extrude Ca2+ from the cell, and deregulation of the intracellular Ca2+ homeostasis is related to several kinds of diseases, including cancer. However, their roles and molecular mechanism in osteosarcoma are elusive.Methods: The expression of miR-193a-5p and NCX2 in osteosarcoma and corresponding adjacent noncancerous tissues was investigated by qRT-PCR. Colony formation assay, wound healing assay, transwell invasion assay and xenograft mouse model were used for in vitro and in vivo functional analyses. Tandem mass tag-based quantitative proteomics analysis was performed to identify the targets of miR-193a-5p.Results: This study showed that miR-193a-5p was upregulated in osteosarcoma tissues compared with the corresponding adjacent noncancerous tissues, and promoted colony formation, migration, invasion, and epithelial-mesenchymal transition (EMT) in vitro, as well as metastasis in vivo. Quantitative proteomics analysis identified NCX2 as a potential target of miR-193a-5p. Luciferase activity assay and Western blotting further confirmed that miR-193a-5p recognized the 3′-untranslated region of NCX2 mRNA, and negatively regulated NCX2 expression. NCX2 was downregulated in osteosarcoma tissues, and its expression was negatively correlated with miR-193a-5p levels. Ectopic expression of NCX2 in osteosarcoma cells could counteract the oncogenic effects of miR-193a-5p. These results indicate that miR-193a-5p exerts its effects by targeting NCX2. Further study demonstrated that NCX2 suppressed Ca2+-dependent Akt phosphorylation by decreasing intracellular Ca2+ efflux, then inhibited EMT process. Treatment with the antagomir against miR-193a-5p sensitized osteosarcoma to the Akt inhibitor afuresertib in a murine xenograft tumor model. Conclusion: This study revealed a miR-193a-5p/NCX2/AKT signaling axis in the progression of osteosarcoma, which may provide a new therapeutic target for osteosarcoma treatment.
Lubrication-Contact Boundary Conditions for Lubrication Problems
Authors: Liu Shuangbiao 
Published: April 2021

Abstract
Under sever conditions, asperity contacts take place, surrounded by fluid lubrication. Mathematical descriptions are needed to describe local connection between fluid lubrication and solid contacts. In order to differentiate them from conventional boundary conditions, these conditions are called lubrication-contact boundary conditions (LCBCs), which have not been sufficiently addressed by previous studies. In this work, a set of LCBCs formulations are constructed based on local flow continuity from the continuum mechanics point of view, together with pressure inequalities. Numerical implementations are developed and tested with problems involving simple geometries, and they are expected to be integrated with mixed/boundary EHL solvers, as well as be applied to deterministic sub-models of stochastic models in order to obtain flow factors that consider solid contacts.
MiR-182-5p Inhibits the Tumorigenesis of clear cell renal cell carcinoma by Repressing UBE2T
Authors: Wu Yucai , Zhang Cuijian , Peng Ding , He Shiming , Huang Cong , Qian Jinqin , Zhu Weijie , Gong Yanqing , Li Xuesong , Zhou Liqun 
Published: April 2021

Abstract
Background: Ubiquitin-conjugating enzyme E2T (UBE2T), a member of the E2 family, has been reported to be overexpressed in certain tumor types and to have an important role in the Fanconi anemia pathway. However, the role of UBE2T in clear cell renal cell carcinoma (ccRCC) has not already been clarified. MicroRNAs (miRNAs) participate in tumorigenesis by binding to genes and proteins that regulate cell proliferation or cell apoptosis. The aim of this research was to determine the role of UBE2T and the relationship between miR-182-5p and UBE2T in ccRCC.Methods: UBE2T expression levels were assessed by real-time quantitative PCR (RT-qPCR) and western blot in ccRCC tissues and cells. Protein expression of UBE2T was assessed in a total of 93 ccRCC patients from Peking University First Hospital (PKU) by immunohistochemistry (IHC). The effects of UBE2T knockdown on ccRCC cells were assessed by MTS assays, wound healing assays, transwell invasion assays and ﬂow cytometry. The effects of in vivo treatment were evaluated through xenograft experiments. The relationship between miR-182-5p and UBE2T was verified by dual luciferase reporter gene assay.Results: UBE2T was highly expressed in ccRCC cells and tissues. High expression of UBE2T was positively correlated with advanced pathological stage, histological grade, maximum diameter and distant metastasis of the tumor. Multivariate analysis revealed that the UBE2T expression was an independent risk factor for overall survival (OS) and recurrence-free survival (RFS) in patients with ccRCC. Knockdown of UBE2T significantly suppressed proliferation, migration and invasion of RCC. Flow cytometry analysis showed that knocking down UBE2T could promote RCC cell cycle arrest at G2/M phase and increase cell apoptosis. A xenograft model also confirmed that suppression of UBE2T significantly delayed tumor formation and growth in vivo. In addition, miR-182-5p inhibited the expression of UBE2T protein through targeting UBE2T mRNA, and then inhibited the proliferation, migration and invasion of ccRCC.Conclusions: Our research reveals that UBE2T probably play a critical role in ccRCC progression, and it may be a potential therapeutic target for ccRCC treatment.
Dynamics and mechanisms of bioaccumulation and elimination of Nonylphenol in Zebrafish
Authors: Zhang Yinjie , Peng Cheng , Zhang Wei , Ling Siyuan , Hu Shuangqing 
Published: April 2021

Abstract
Nonylphenol (NP) has been widely concerned for its endocrine disrupting effects and bioconcentration in aquatic organisms. However, the knowledge on the dynamic uptake and depuration in model organisms - zebrafish (Danio rerio) were still limited. In this study, we investigated the accumulation and elimination of NP for the whole body and trunk of zebrafish by a modified pretreatment and analysis method with fewer samples. The results of acute toxicity test showed that the LC50 values of NP in zebrafish ranged from 474 µg·L− 1 for a 24-h exposure to lower than 238 µg·L− 1 for a 96-h exposure. Meanwhile, the NP concentrations in zebrafish during the depuration stage fitted the first-order kinetic model well, and the depuration rate constant (K2) was reduced with higher NP concentrations. Both the accumulation and elimination of NP in the trunk were faster than those in the whole fish, indicating the preferential transfer from viscera to muscle and rapidly diffusion in reverse. The bioconcentration factors at a steady state (BCFSS) of NP were 104–112 L·kg− 1 in the whole body and 76–104 L·kg− 1 in the trunk, respectively, suggesting that the muscle was a major position for NP storage. The BCFSS were increased within a certain concentration due to the decreasing depuration capacity inhibited by NP toxicity. To our knowledge, this study was the first to emphasize the correlation between NP exposure concentration and bioconcentration capacity.
BPA in Baby Feeding PET Bottle, Aluminium Can and Thermal Printed Paper: Application of Acridine Orange Oxidation Inhibition Method to Estimate BPA
Authors: Kapse Shruti S. , Prasad Kumar Suranjit , Prasad Bablu 
Published: April 2021

Abstract
The leaching of Bisphenol-A (BPA) from plastic baby feeding bottles, aluminium cans and thermal printed receipts was investigated under aquatic condition at high temperature. Leached BPA was analysed using UV-Visible Spectrophotometer based on inhibitory effect of BPA on acridine orange oxidation, as a function of change in temperature and time of contact of water with the samples. Proposed method of BPA estimation method does not require any extraction and/or derivatization. The detection limit of BPA under current experimental setup was 0.1 ng/ml. The results of BPA leached from baby feeding PET bottles, aluminium can with epoxy resin lining and thermal paper were 87 ± 10 ng/bottles, 68 ± 5 ng/bottle and 110 ± 15 ng/receipt under the current experimental conditions.
The Underlying Molecular Mechanisms and Prognostic Factors of Rna Binding Protein in Colorectal Cancer: A Study Based on Multiple Online Databases
Authors: He Qinglian , Li Ziqi , Lei Xue , Zou Qian , Yu Haibing , Ding Yuanlin , Zhu Wei 
Published: April 2021

Abstract
Background: RNA binding protein (RBP) is an active factor involved in the occurrence and development of colorectal cancer (CRC). Therefore, the potential mechanism of RBP in CRC needs to be clarified by dry-lab analyses or wet-lab experiments.Methods: The differential RBP gene obtained from the GEPIA 2 (Gene Expression Profiling Interactive Analysis 2) were performed functional enrichment analysis. Then, the alternative splicing (AS) events related to survival were acquired by univariate regression analysis, and the correlation between RBP and AS was analyzed by R software. The online databases were conducted to analyze the mutation and methylation of RBPs in CRC. Moreover, 5 key RBP signatures were obtained through univariate and multivariate Cox regression analysis and established as RBP prognosis model. Subsequently, the above model was verified through another randomized group of TCGA CRC cohorts. Finally, multiple online databases and qRT-PCR analysis were carried to further confirm the expression of the above 5 RBP signatures in CRC.Results: Through a comprehensive bioinformatics analysis, it was revealed that RBPs had genetic and epigenetic changes in CRC. We obtained 300 differentially expressed RBPs in CRC samples. The functional analysis suggested that they mainly participated in spliceosome. Then, a regulatory network for RBP was established to participate in AS and DDX39B was detected to act as a potentially essential factor in the regulation of AS in CRC. Our analysis discovered that 11 differentially expressed RBPs with a mutation frequency higher than 5%. Furthermore, we found that 10 differentially expressed RBPs had methylation sites related to the prognosis of CRC, and a prognostic model was constructed by the 5 RBP signatures. In another randomized group of TCGA CRC cohorts, the prognostic performance of the 5 RBP signatures was verified. Conclusion: The potential mechanisms that regulate the aberrant expression of RBPs in the development of CRC was explored, a network that regulated AS was established, and the RBP-related prognosis model was constructed and verified, which could improve the individualized prognosis prediction of CRC.
The Effect Of Body Composition On Strenght An Power In Male And Female Students
Authors: Ghassen Ben Mansour , Asma Kacem , Mohamed Ishaq , Laurent Grélot , Foued Ftaiti 
Published: April 2021

Abstract
Purpose - The Aim of this study is to determine and to compare the impact of fat excess on neuromuscular performances during short maximal exercise in male and female students. Methods - 72 male and 64 female students aged 20 to 23 years were enrolled in this study. After assessing their morphological characteristics (body mass, height and percentage of fat mass), a squat jump test (SJ), a 5 successive jump test (5JT), a hand gripping (HG) and back strength (BS) tests have been conducted for each subject. Male students were re-tested after being weighed down with a weight equivalent to the mean differences in body fat recorded between the two sexes in the form of a loaded worn vest. Results – Male are 15.7% heavier and 7.4% taller and presented a percentage of fat mass (17.2 ± 1.8%) significantly (p<0.001) lower than that of women subject (25.0 ± 2.5%) (difference male vs female for fat mass: -45.5%). HG, BS, 5JT and SJ performances were significantly higher in males (44±5 kg, 141±2 kg, 11±1 m and 32.4 ± 2,7 cm, respectively) than in females (31.0±4 kg, 81.6±13 kg, 8.7 ± 0.7 m and 21.1 ± 1.9 cm, respectively. In the control (unloaded) condition, the relative difference between males and females represented 23.5% and 34.7% of the male performances for 5JT and SJ, respectively. In the weighted condition, the relative difference between weighted males and females still represented 11.7% and 23.8% of the weighted male performances for 5JT and SJ, respectively. Cancelling the sex difference in fat mass by adding weight in males reduced by 50.1% the sex difference during 5JT and 31.4% and 71.7% for hight and power results, respectively during SJ test. Conclusion – During short and maximal exercise, male performed better with their hands, back and legs than female students. Excess fat for female students have a disadvantageous effect on vertical and horizontal jumps performances. The persistence of sex differences after weighting of male students indicates that body fat is responsible for 30 to 70 % of the observed differences between sexes performances and power outcome during jump tests.
Atrazine induced toxic impacts on the survival, behavior, and biochemical aspects of Silver carp (Hypophthalmichthys molitrix)
Authors: Nayab Naireen , Shah Syed Ata Ur Rahman , Ullah Sana , Rauf Muhammad , Ali Shandana , Khan Shahid Niaz , Azizullah Azizullah , Dawar Farman Ullah 
Published: April 2021

Abstract
The current research work was conducted to evaluate the acute toxicity of the widely used herbicide, Atrazine on the behavioral responses and biochemical changes in different tissues of Silver carp (Hypophthalmichthys molitrix). Adult fish were exposed to different concentrations of Atrazine for different time periods. Mortality of fish was observed with increase in the concentration of Atrazine and time of exposure. The LC50 of Atrazine for 96 hours was calculated as 13.7µl/L. Fish exposed to Atrazine showed fast swimming, loss of equilibrium and balance, gulping, pale body coloration, and an excess amount of mucous secretion covered the buccal cavity and gills. Total protein contents, antioxidant enzymes including catalase (CAT), peroxidase (POD), and glutathione reductase (GR) and lipid peroxidation (LPO), were measured in the brain, gills, liver, and muscle tissues for biochemical analysis. Atrazine exposure resulted in a significant time-dependent decrease in total protein content in the liver, gills, brain, and muscle tissue of fish compared to the control group. A slight increase was observed in the activities of CAT and POD in the control group, while a significant decrease was observed in the ATZ-treated groups from 24 to 96 h. The glutathione reductase activity was significantly increased in the ATZ treated groups compared to control group. Likely, LPO level also increased in the liver, gills, brain, and muscle tissue in a time-dependent and concentration-dependent manner in ATZ treated groups compared to control group. Concludingly, our results revealed that Atrazine is toxic to fish, alter enzymes and total protein level, and lipid peroxidation level in fish. This harmful effect of Atrazine maybe generalized into environment and reduce the Silver carp population in the natural freshwater bodies.