bluuebunny/crossref_metadata_2025_split huggingface dataset:
From Headhunting to Head Taxes
Authors: Manse Maarten 
Published: November 2021

Abstract
This article investigates Dutch colonial practices on the Moluccan island of Seram in the late nineteenth and early twentieth centuries. Seram’s mountainous interior was the domain of ungoverned, peripatetic Alfurs who engaged in headhunting. For a long time, they were rendered untouched by colonialism and administered through coastal intermediaries. After 1900, renewed imperial-civilizational vigour demanded the direct incorporation and ‘civilization’ of Seram’s stateless spaces. A series of expeditions subjected the Alfurs to registration, categorization, and taxation, which this article argues were seen as pivotal, moralizing tools of colonial social-engineering, used to inscribe subjected people into the state and instil compliant and ‘productive’ behaviour. However, rather than a replacement of indigenous orders with European modernity, colonization produced a hybrid fusion of colonial strategies of domination with indigenous cultural practices of state-evasion. This article demonstrates that colonial governance was a site of interaction, in which colonial developmentalism and modernity were actively negotiated and challenged.
Invisible Resilience: Indigenous Knowledge Systems of Earthquake Disaster Management in Kagera Region, Tanzania
Authors: Hambati Herbert 
Published: November 2021

Abstract
Indigenous environmental disaster management systems are maintained in remotely located communities through intergenerational transmission. This fieldwork illuminates a wealth of unprecedented survival techniques, strategies and skills for offsetting the worst effects of a natural disaster in earthquake-prone areas of north-west Tanzania. Normally, responses from the government and its foreign development partners are hamstrung by bureaucratic red tape, taking too long in reaching disaster victims to be of any actual help. The formal mechanisms of global assistance constitute disaster management failure by design. Rather, it is the local experts who sustain human lives in the weeks and months before external aid comes to the rescue. Yet local communities’ contributions to their own survival remain invisible to central government and the global arena. Traditional means of forecasting environmental catastrophes and of providing essential assistance in the aftermath of natural disasters are reflections of cultural values, socio-economic sophistication and scientific expertise within communities whose resilience needs to be recognized, assisted and promoted. Educational curricula of the future, involving a new generation of academicians, should integrate this crucial indigenous knowledge into the nation’s mainstream disaster management framework.
Architects of the Engaruka Techno-Cultural Complex: Testing the Sonjo Connection
Authors: Kadalida Penina E. 
Published: November 2021

Abstract
Engaruka is an archaeological site that became known to the world of scientific researchers for the first time in 1883. Since then the site has been the subject of many research undertakings varying in purpose and intensity. Most of the published literature about Engaruka has focused on its economy, technology, population, probable reasons for its success and demise, as well as speculations about its first settlers. Several different ethnic groups have been proposed as Engaruka’s architects: the Iraqw, Tatoga, Maasai, and the Sonjo. Despite the impressive scope of collected evidence, the original occupants of Engaruka have yet to be determined conclusively. The analysis of available evidence assembled here supports the hypothesis that the Sonjo people were the creators of Engaruka, by virtue of these indicators: (i) terrace patterns, (ii) pottery technologies, (iii) stone structures, (iv) fire places, and (v) contemporary ethnography.
On the authorship and publication dates of Theraphosa and Theraphosidae (Araneae, Mygalomorphae): a clarification
Authors: BREITLING RAINER 
Published: November 2021

The genus Theraphosa was established by Thorell (1870) as the type genus of the simultaneously published family Theraphosidae, the most diverse group of mygalomorph spiders. This authorship and publication date have long been accepted by the majority of authors. However, there has been a long-standing minority view that the genus name should be attributed to Walckenaer (1805), and the publication date of the family name changed to 1869.
A thought-provoking recent publication has examined this case. Based on a limited selection of the relevant literature, the authors struggled to make sense of their sources and prematurely concluded that the minority opinion might indeed be correct. They overlooked the potentially destabilising implications of this reattribution.
This paper revisits the evidence in the light of a much wider range of relevant publications, places it in its important historical context and, on the basis of the current rules of nomenclature, concludes that the traditional consensus has indeed been correct.
Thus, Theraphosa Thorell, 1870 is the type genus of Theraphosidae Thorell, 1870 and a nomen protectum, while Theraphosa Schinz, 1823 is a nomen oblitum, mostly limited to the German textbook literature of the early 19th century. Teraphosa Eichwald, 1830 and Teraphosa Gistel, 1848 are junior synonyms of Avicularia Lamarck, 1818 (syn. nov.). Theraphosa Walckenaer, 1805 is a suprageneric name of the class-series (synonymous to Mygalomorphae) and not available at the genus level.
Feasibility of Intradialytic Cycling Program in a Remote Community Hemodialysis Unit: Mixed-Methods Analysis of Implementation
Authors: Kaminska Malgorzata E. , Roots Robin K. , Singh Anurag 
Published: November 2021

Background: There is growing evidence demonstrating the benefits of intradialytic cycling. However, there are relatively few centers where this practice has been adopted with no reports from hemodialysis units in rural, remote, and northern locations. Maintaining mobility and quality of life for patients on kidney replacement therapy living in remote northern communities is inhibited by inclement weather and lack of access to resources and infrastructure that support physical activity. The integration of intradialytic cycling during hemodialysis offers patients a form of safe physical activity year-round. Objective: This study focuses on better understanding the feasibility and acceptability of implementing intradialytic cycling in a remote northern geographical context. Design: A feasibility study using a mixed-methods explanatory design was adopted for this study. Setting: The research is conducted in Prince George, British Columbia. Participants: The participants are patients attending a community-based dialysis unit in remote northern British Columbia and health professionals working in the same facility. Methods: Quantitative measures were captured through cycling logbooks and quality of life measure, and qualitative data were obtained through semi-structured interviews and analyzed using thematic analysis. Results: Six (43%) eligible patients used leg ergometers more than once for a median of 2.5 (interquartile range: 1-4) months and 87% of hemodialysis sessions. Participants cycled for a median of 65 (interquartile range: 39-76) minutes per session, with frequent variability noted between participants and different hemodialysis sessions for the same participant. Nine patients completed the European Quality of Life Health Questionnaire prestudy, with 5 (56%) also completing it poststudy. Interviews with 9 patients, 4 nurses, and 1 physiotherapist led to the identification of themes instrumental to implementation: a supportive community dialysis unit, shared responsibility, knowledge of patients/providers, and benefits associated with engagement. Themes that were identified as being key to acceptability in this remote dialysis unit were trust, connection, and engagement through common values. Limitations: Due to dialysis unit size, we had a small number of participants. Conclusions: This study demonstrates the feasibility of implementing best practice in a remote community and provides insight into the elements of context and participation that contribute to acceptability in the implementation of intradialytic cycling.
Clinical Outcomes and Postoperative Complications After All-Epiphyseal Double-Bundle ACL Reconstruction for Skeletally Immature Patients
Authors: Sasaki Shizuka , Sasaki Eiji , Kimura Yuka , Yamamoto Yuji , Tsuda Eiichi , Ishibashi Yasuyuki 
Published: November 2021

Background: The treatment strategy for pediatric anterior cruciate ligament (ACL) tears, especially in patients with open physes, remains controversial. Purpose: To assess clinical outcomes and postoperative complications after all-epiphyseal double-bundle ACL (DB-ACL) reconstruction for patients with open physes. Study Design: Cohort study; Level of evidence, 3. Methods: Included were 102 patients aged ≤15 years who underwent ACL reconstruction at a single institution and had a minimum of 2 years of follow-up. Of these patients, 18 had undergone all-epiphyseal DB-ACL reconstruction (mean age, 12.4 ± 1.2 year) and 84 had undergone conventional DB-ACL reconstruction (mean age, 14.1 ± 0.9 year). The outcomes of the all-epiphyseal group were compared with those of the conventional group. Objective clinical outcomes included KT-1000 arthrometer measurements of side-to-side difference in anterior tibial translation, Lachman test grade, and pivot-shift test grade. Radiographic angular deformity (defined as >3° of the side-to-side difference in femorotibial angle) and incidence of second ACL injury were also compared. Results: The postoperative side-to-side difference in laxity significantly improved from 6.1 ± 2.4 to 0.6 ± 0.9 mm in the all-epiphyseal group ( P = .001), and postoperative laxity was similar to that of the conventional group (0.4 ± 0.8 mm; P = .518). A Lachman grade 1 positive result was observed in 20% of the all-epiphyseal group and 3% of the conventional group ( P = .042), and a pivot-shift grade 1 positive result was observed in 22% of the all-epiphyseal group and 4% of the conventional group ( P = .074). A total of 4 patients (26.7%) in the all-epiphyseal group and 4 (6.1%) in the conventional group demonstrated angular deformity ( P = .035). The incidence of postoperative ipsilateral ACL tear was 16.7% in the all-epiphyseal group and 23.8% in the conventional group ( P = .757). The incidence of contralateral ACL tear was 11.1% in the all-epiphyseal group and 14.3% in the conventional group ( P ≥ .999). Conclusion: All-epiphyseal DB-ACL reconstruction for skeletally immature patients achieved satisfactory clinical outcomes compared with conventional DB-ACL reconstruction. The incidence of ipsilateral graft rupture was relatively high in both groups. The all-epiphyseal group had a significantly higher incidence of angular deformity.
Preparation of nanoparticles of β-cyclodextrin-loaded scutellarein anti-tumor activity research by targeting integrin αvβ3
Authors: Wang Jundong , Li Tianhao , Yue Chaochi , Zhong Sen , Yang Xiangdong , Li Jun , Li Yuanzhi 
Published: November 2021

Abstract
Background
The problems associated with the poor water solubility of anticancer drugs are one of the most important challenges in achieving effective cancer therapy. The present study was designed to evaluate the effect of scutellarein on human colon cancer cells in vitro by using a target αvβ3 novel scutellarein (Scu)-loaded niosome nanoparticle (β-CD-CL-Scu-cRGD).

Results
β-CD-CL-Scu-cRGD has a diameter of 140.2 nm and zeta potential of − 11.3 mV with constant physicochemical stability. The MTT assay showed both Scu and β-CD-CL-Scu-cRGD caused a decrease in cell proliferation and viability of LoVo, but β-CD-CL-Scu-cRGD showed better activity in vitro. Colony formation assay and flow cytometry assay showed that β-CD-CL-Scu-cRGD has a better effect on cell proliferation and apoptosis. In vivo, animal experimental results showed that β-CD-CL-Scu-cRGD can significantly inhibit tumor growth, and the bodyweight of mice decreases during the treatment of scutellarein and its derivatives. β-CD-CL-Scu-cRGD could inhibit the protein levels of Ki67 and αvβ3, thereby inhibiting tumor growth.

Conclusions
Although further in vitro and in vivo studies are necessary, our results suggested that β-CD-CL-Scu-cRGD could be an outstanding carrier to deliver Scu for potential therapeutic approaches into colon cancer.
Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues
Authors: Jia Erteng , Shi Huajuan , Wang Ying , Zhou Ying , Liu Zhiyu , Pan Min , Bai Yunfei , Zhao Xiangwei , Ge Qinyu 
Published: November 2021

Abstract
Background
Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input RNA or subcellular sequencing.

Results
Here, we present a highly sensitive library construction protocol for ultralow input RNA sequencing (ulRNA-seq). We systematically evaluate experimental conditions of this protocol, such as reverse transcriptase, template-switching oligos (TSO), and template RNA structure. It was found that Maxima H Minus reverse transcriptase and rN modified TSO, as well as all RNA templates capped with m7G improved the sequencing sensitivity and low abundance gene detection ability. RNA-seq libraries were successfully prepared from total RNA samples as low as 0.5 pg, and more than 2000 genes have been identified.

Conclusions
The ability of low abundance gene detection and sensitivity were largely enhanced with this optimized protocol. It was also confirmed in single-cell sequencing, that more genes and cell markers were identified compared to conventional sequencing method. We expect that ulRNA-seq will sequence and transcriptome characterization for the subcellular of disease tissue, to find the corresponding treatment plan.
Sampling the fish gill microbiome: a comparison of tissue biopsies and swabs
Authors: Clinton Morag , Wyness Adam J. , Martin Samuel A. M. , Brierley Andrew S. , Ferrier David E. K. 
Published: November 2021

Abstract
Background
Understanding the influence of methodology on results is an essential consideration in experimental design. In the expanding field of fish microbiology, many best practices and targeted techniques remain to be refined. This study aimed to compare microbial assemblages obtained from Atlantic salmon (Salmo salar) gills by swabbing versus biopsy excision. Results demonstrate the variation introduced by altered sampling strategies and enhance the available knowledge of the fish gill microbiome.

Results
The microbiome was sampled using swabs and biopsies from fish gills, with identical treatment of samples for 16S next generation Illumina sequencing. Results show a clear divergence in microbial communities obtained through the different sampling strategies, with swabbing consistently isolating a more diverse microbial consortia, and suffering less from the technical issue of host DNA contamination associated with biopsy use. Sequencing results from biopsy-derived extractions, however, hint at the potential for more cryptic localisation of some community members.

Conclusions
Overall, results demonstrate a divergence in the obtained microbial community when different sampling methodology is used. Swabbing appears a superior method for sampling the microbiota of mucosal surfaces for broad ecological research in fish, whilst biopsies might be best applied in exploration of communities beyond the reach of swabs, such as sub-surface and intracellular microbes, as well as in pathogen diagnosis. Most studies on the external microbial communities of aquatic organisms utilise swabbing for sample collection, likely due to convenience. Much of the ultrastructure of gill tissue in live fish is, however, potentially inaccessible to swabbing, meaning swabbing might fail to capture the full diversity of gill microbiota. This work therefore also provides valuable insight into partitioning of the gill microbiota, informing varied applications of different sampling methods in experimental design for future research.
Transcriptome analysis provides insights into the root response of Chinese fir to phosphorus deficiency
Authors: Chen Wanting , Zhou Mengyan , Zhao Mingzhen , Chen Ranhong , Tigabu Mulualem , Wu Pengfei , Li Ming , Ma Xiangqing 
Published: November 2021

Abstract
Background
Phosphorus is one of the essential elements for plant growth and development, but available phosphorus (Pi) content in many soil types is low. As a fast-growing tree species for timber production, Chinese fir is in great demand of Pi, and the lack of Pi in soil restricts the increase of productivity of Chinese fir plantation. Root morphology and the synthesis and secretion of organic acids play an important role in the uptake of phosphorus, but the molecular mechanisms of Chinese fir root responses to Pi deficiency are largely unexplored. In this study, seedlings of Yang 061 clone were grown under three Pi supply levels (0, 5 and 10 mg·L-1 P) and morphological attributes, organic acid content and enzyme activity were measured. The transcriptome data of Chinese fir root system were obtained and the expression levels of phosphorus responsive genes and organic acid synthesis related genes on citric acid and glyoxylate cycle pathway were determined.

Results
We annotated 50,808 Unigenes from the transcriptome of Chinese fir roots. Among differentially expressed genes, seven genes of phosphate transporter family and 17 genes of purple acid phosphatase family were up-regulated by Pi deficiency, two proteins of SPX domain were up-regulated and one was down-regulated. The metabolic pathways of the citric acid and glyoxylate cycle pathway were mapped, and the expression characteristics of the related Unigenes under different phosphorus treatments were analyzed. The genes involved in malic acid and citric acid synthesis were up-regulated, and the activities of the related enzymes were significantly enhanced under long-term Pi stress. The contents of citric acid and malic acid in the roots of Chinese fir increased after 30 days of Pi deficiency.

Conclusion
Chinese fir roots showed increased expression of genes related with phosphorus starvation, citrate and malate synthesis genes, increased content of organic acids, and enhanced activities of related enzymes under Pi deficiency. The results provide a new insight for revealing the molecular mechanism of adaption to Pi deficiency and the pathway of organic acid synthesis in Chinese fir roots.
Placental pathology in a pregnant woman with severe COVID-19 and successful ECMO treatment: a case report
Authors: Rosner-Tenerowicz Anna , Fuchs Tomasz , Zimmer-Stelmach Aleksandra , Pomorski Michał , Trzeszcz Martyna , Zwierzchowski Jacek , Zimmer Mariusz 
Published: November 2021

Abstract
Background
Infection with SARS-CoV-2 during pregnancy can lead to a severe condition in the patient, which is challenging for obstetricians and anaesthesiologists. Upon severe COVID-19 and a lack of improvement after multidrug therapy and mechanical ventilation, extracorporeal membrane oxygenation (ECMO) is introduced as the last option. Such treatment is critical in women with very preterm pregnancy when each additional day of the intrauterine stay is vital for the survival of the newborn.

Case presentation
We report a case of a 38-year-old woman at 27 weeks of gestation treated with multidrug therapy and ECMO. The woman was admitted to the intensive care unit (ICU) with increasing fever, cough and dyspnoea. The course of the pregnancy was uncomplicated. She was otherwise healthy. At admission, she presented with severe dyspnoea, with oxygen saturation (SpO2) of 95% on passive oxygenation, heart rate of 145/min, and blood pressure of 145/90. After confirmation of SARS-CoV-2 infection, she received steroids, remdesivir and convalescent plasma therapy. The foetus was in good condition. No signs of an intrauterine infection were visible. Due to tachypnea of 40/min and SpO2 of 90%, the woman was intubated and mechanically ventilated. Due to circulatory failure, the prothrombotic activity of the coagulation system, further saturation worsening, and poor control of sedation, she was qualified for veno-venous ECMO. An elective caesarean section was performed at 29 weeks on ECMO treatment in the ICU. A preterm female newborn was delivered with an Apgar score of 7 and a birth weight of 1440 g. The newborn had no laboratory or clinical evidence of COVID-19. The placenta showed the following pathological changes: large subchorionic haematoma, maternal vascular malperfusion, marginal cord insertion, and chorangioma.

Conclusions
This case presents the successful use of ECMO in a pregnant woman with acute respiratory distress syndrome in the course of severe COVID-19. Further research is required to explain the aetiology of placental disorders (e.g., maternal vascular malperfusion lesions or thrombotic influence of COVID-19). ECMO treatment in pregnant women remains challenging; thus, it should be used with caution. Long-term assessment may help to evaluate the safety of the ECMO procedure in pregnant women.
The development and validation of the psychological capital questionnaire for patients with Cancer the psychological capital questionnaire
Authors: Cui Chun Ying , Wang Yu , Zhang Ying , Chen Siqi , Jiang Nan , Wang Lie 
Published: November 2021

AbstractBackgroundStudies increasingly show that positive psychological constructs affect the mental health of cancer patients. However, most scales that measure hope, resilience, optimism and self-efficacy have been developed based on general populations. The aim of our study was to develop a psychological capital (PsyCap) questionnaire for patients with cancer (PCQ-C) to gauge their mental state more accurately.MethodsThe items for the scale were selected by comprehensive literature review and semi-structured interviews, and the relevant terms were screened by an expert panel. A pilot study was then conducted on 202 patients to reduce the item pool, and the reliability and validity of the scale were evaluated using 500 completed questionnaires. The test-retest reliability was then assessed using a subsample of 100 patients. Finally, the completed questionnaires of 229 patients with breast cancer were used to assess the criterion validity of the PCQ-C, including measures of depression and anxiety.ResultsItem reduction and exploratory factory analysis resulted in 24 items for self-efficacy, hope, resilience and optimism, accounting for 56.72% of the variance. The Cronbach’s alpha for the scale was 0.886, and the test-retest reliability was 0.825. PsyCap showed a significant negative correlation with both depression (r = − 0.631,P < 0.01) and anxiety (r = − 0.601,P < 0.01).ConclusionThe PCQ-C can objectively evaluate PsyCap in cancer patients and exhibits good psychometric properties.