bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERUBAHAN POLA PENGELOLAAN HUTAN OLEH MASYARAKAT DI DESA KALIGUNTING (Studi Kasus PHBM di Desa Kaligunting, Kecamatan Mejayan, Kabupaten Madiun, Jawa Timur)
Authors: Annisa Winanda Rizky , Zunariyah Siti 
Published: February 2021

<h1>Abstract : The existence of forest in Indonesia nowadays could be identified as under critical condition. It was not only caused by illegal logging phenomenon, but also the habit of forest village community which implicitly gives contribution towards the decay of forest ecosystem. This research used qualitative-descriptive method with approach case studies. The change that occurred in Kaligunting Village could be identified as two aspect, including physical aspect and non-physical aspect. Related to physical aspect, cultivating pattern that turned to 3m x 3m impacted on forest sustainability and level of society participation in tumpangsari. Whereas, non-physical aspect change, including social change, economics change, and cultural change. Social change could be seen by the emergence of LMDH Sumber Tani which made level of participation, interaction and social distance, and social network increased. Economic change is visible through the emergence of business opportunity such as cassava flour which became main product of the village, up to the existence of production sharing form Perum Perhutani. Then, in the cultural aspect, the change is occurred in cultivation and harvesting ritual.</h1><h1><strong style="font-size: 10px;">Keyword : </strong><span style="font-size: 10px;">Social change, forest management, Forestry Departmen, PHBM, civil institutions</span></h1><p> </p><p><strong>Abstrak</strong><strong> </strong>: Keberadaan hutan di Indonesia saat ini dapat diidentifikasi karena berada dalam kondisi kritis. Bukan hanya disebabkan oleh fenomena illegal logging, tetapi juga kebiasaan masyarakat desa hutan yang secara implisit memberikan kontribusi terhadap kerusakan ekosistem hutan. Penelitian ini menggunakan metode kualitatif-deskriptif dengan pendekatan studi kasus. Perubahan yang terjadi di Desa Kaligunting dapat diidentifikasi sebagai dua aspek, termasuk aspek fisik dan non-fisik. Terkait dengan aspek fisik, pola budidaya yang berubah menjadi 3m x 3m berdampak pada kelestarian hutan dan tingkat partisipasi masyarakat dalam tumpangsari. Sedangkan, aspek non fisik berubah, termasuk perubahan sosial, perubahan ekonomi, dan perubahan budaya. Perubahan sosial dapat dilihat dengan munculnya LMDH Sumber Tani yang membuat tingkat partisipasi, interaksi dan jarak sosial, dan jejaring sosial meningkat. Perubahan ekonomi terlihat melalui munculnya peluang bisnis seperti tepung ubi kayu yang menjadi produk utama desa, hingga adanya bentuk bagi hasil Perum Perhutani. Kemudian, dalam aspek budaya, perubahan itu terjadi dalam ritual kultivasi dan panen.</p><p><br /> <strong>Kata Kunci</strong>: Perubahan sosial, pengelolaan hutan, Departemen Kehutanan, PHBM, lembaga sipil</p>
RISIKO DAN REFLEKSIVITAS GAY TERHADAP KEKERASAN (Studi Kasus pada Komunitas Gay di Surakarta)
Authors: Rahayu Vebrianti , Demartoto Argyo 
Published: February 2021

<p>Gay is a minority group the existence of which is still unacceptable to the society. It puts the gay into a group vulnerable to violence risk. The objective of research is to study the risk and the reflexivity of violence in gay community in Surakarta. This qualitative with case study approach took place in Surakarta. The sampling technique used was purposive sampling one. The informant of research consisted of chairperson and administrators of Surakarta Gaya Mahardhika Foundation, gays becoming the victim of violence, community fellow members, and administrators of Mitra Alam Surakarta NGO. Data was collected through observation, in-depth interview, and documentation. To validate data, source triangulation was used. Technique of analyzing data used was Miles and Huberman’s interactive model of analysis with Ulrich Beck’s Risk Society theory. </p><p>The result of research showed that violence risk encountered by gay in Surakarta included physical violence such as being thrown with sharp weapon, being struck and slapped; physical/emotional violence such as cynical and disliking view, expulsion, threat and negative stigma intended to gay organization; sexual violence such as sexual abuse conducted by police officer by touching the victim’s body organ; economic violence such as money and product expropriation by sexual partner; and verbal violence such as being insulted and mocked by some people on the street. The effect of violence consisted of physical effect such as bruise, gash, and pain still felt until today, and physical effect such as fear, trauma, discomfort and fidget, disappointment, resentfulness, anger, pique, and regret. The reflectivity of gay in dealing with violence included interacting or looking for acquaintance or partner more alertly and selectively; adapting, comporting; building intimacy an self-image by conducting positive activities within society; conducting homosexual activity more carefully such as fulfilling their sexual need in their known place rather than doing it in opened space that has been known by mass organization; and providing advocacy to the gay becoming the victim of violence. </p>
RISIKO DAN REFLEKSIVITAS GAY TERHADAP KEKERASAN (Studi Kasus pada Komunitas Gay di Surakarta)
Authors: Rahayu Vebrianti , Demartoto Argyo 
Published: February 2021

<p>Gay is a minority group the existence of which is still unacceptable to the society. It puts the gay into a group vulnerable to violence risk. The objective of research is to study the risk and the reflexivity of violence in gay community in Surakarta. This qualitative with case study approach took place in Surakarta. The sampling technique used was purposive sampling one. The informant of research consisted of chairperson and administrators of Surakarta Gaya Mahardhika Foundation, gays becoming the victim of violence, community fellow members, and administrators of Mitra Alam Surakarta NGO. Data was collected through observation, in-depth interview, and documentation. To validate data, source triangulation was used. Technique of analyzing data used was Miles and Huberman’s interactive model of analysis with Ulrich Beck’s Risk Society theory. </p>The result of research showed that violence risk encountered by gay in Surakarta included physical violence such as being thrown with sharp weapon, being struck and slapped; physical/emotional violence such as cynical and disliking view, expulsion, threat and negative stigma intended to gay organization; sexual violence such as sexual abuse conducted by police officer by touching the victim’s body organ; economic violence such as money and product expropriation by sexual partner; and verbal violence such as being insulted and mocked by some people on the street. The effect of violence consisted of physical effect such as bruise, gash, and pain still felt until today, and physical effect such as fear, trauma, discomfort and fidget, disappointment, resentfulness, anger, pique, and regret. The reflectivity of gay in dealing with violence included interacting or looking for acquaintance or partner more alertly and selectively; adapting, comporting; building intimacy an self-image by conducting positive activities within society; conducting homosexual activity more carefully such as fulfilling their sexual need in their known place rather than doing it in opened space that has been known by mass organization; and providing advocacy to the gay becoming the victim of violence.
Comments: Rolling Into 2021 - Take the Time To Adjust Your Worldview
Authors: Boschee Pam 
Published: February 2021

We enter the new year together as an industry and as SPE members, but also as individuals who have traveled on varying roads and experienced personal detours. While this also can be said of years past, 2020 brought with it unanticipated upheavals within the oil and gas industry, global societies, and our personal and professional lives (and livelihoods).
Outlooks for the coming year depend in large part on your own worldview: Are you generally an optimist or a pessimist? An optimist believes that problems are temporary and will get better. A pessimist is convinced that the problem is here to stay and can only get worse. Optimists go into new situations with high expectations, while pessimists hang onto low expectations to prepare for negative outcomes.
Do you see the glass as half full or half empty?
The objective truth is that the water in the glass is at the halfway mark. The rest is up to our interpretation of that truth. The truth itself doesn’t change, but how we interpret it can have a huge effect on our actions.
In his column this month, SPE President Tom Blasingame, a self-described optimist, wrote, “It’s time to look at the horizon” and “open our sails.” The metaphor describes taking action after the worst of a storm has passed or is passing and to make adjustments to get back on course.
None of us are continuously optimistic or pessimistic. Life happens and moves the needle in either direction, but opening our sails may help us recover our optimism when it falters. Tapping into our resources is critical to our worldview.
Am I consistently a cheerleader with a rosy view, no matter what happens? Certainly not, but I tap into my resources and mightily try to move the needle back toward optimism. (Warning: Success in doing so may not be immediate.)
Your personal resources vary, and this is a reminder that SPE is one of those resources.
This list is intended to serve as an “SPE Guide to Optimism.” These offerings can help to move the needle for you. Best wishes for 2021 from the JPT editorial team.
Combination and Improvement of Conventional DNA Extraction Methods in Actinobacteria
Authors: babadi Zahra Khosravi , Narmani Abolfazl , Ebrahimipour Gholam Hossein , Wink Joachim 
Published: February 2021

Abstract
I. Background: DNA extraction is an important step of any molecular experiment, in conjunction with the members of Actinomycetes, DNA couldn’t be quickly extracted by the usual methods of lysis. Due to the low efficiency of most of the conventional DNA extraction techniques, development of an effective techniques for DNA extraction of Actinobacteria in emergency case seems to be necessary. Since, most of the known DNA extraction techniques and commercial kits do not have sufficient efficiency in the extraction of DNA from a different group of Actinobacteria, the objective of this study was to improvement an efficient method from conventional methods for DNA extraction from Actinobacteria. II. Methods and Results: For this purpose, DNA extraction was performed by five methods (an improvedmethod, Invisorb Spin Plant Mini Kit, EZ-10 Spin Column, phenol-chloroform method and Kirby Bauer's method). To evaluate the quantity and quality of extracted genomic DNA, UV absorbance of all samples and efficiency of Polymerase Chain Reaction (PCR) were evaluated. Overall, the results showed the highest quantity of DNA (up to 4000 ng/µl) was obtained by employing introduced DNA extraction method, also yielding good quality. III. These results indicate the recently introduced improved method is more efficient for extraction of DNA from Actinobacteria for DDH (DNA–DNA hybridization) test and for those they require the high concentration of DNA.
Cold storage induces rat liver pyroptosis by activating endoplasmic reticulum stress response through the ATF6-CHOP pathway
Authors: liu jiang , Liang Tiancheng , Ni Yong , Luo De , Li Bo , Gan Yu , Fang Cheng , Ou Santao , Su Song 
Published: February 2021

Abstract
Aims :Liver injury is a common complication of cold storage (CS), and often constitutes a direct cause for liver transplantation failure. The cellular and molecular mechanisms underlying CS-induced liver injury remain unclear. Recent evidence indicates that pyroptosis plays an important role in multiple pathophysiological processes. Using rat liver tissue and cells as a model, we identified a novel mechanism by which inflammasome-dependent interleukin-1β (IL-1β) activation and hepatocyte pyroptosis mediate CS-induced liver injury.Methods :To induce CS, liver tissue and cells were subjected to storage at 4ºC for 12 and 24 h. Inhibition of endoplasmic reticulum (ER) stress was achieved by RNA silencing. Measurements of caspase-1, caspase-11, and IL-1β were performed.Results: Pyroptosis was activated in CS-treated livers, as evidenced by increased levels of caspase-1 and caspase-11 activity, and the elevated expression of IL-1β. ER stress response was activated as well. Inhibition of ER stress response prevented CS-induced liver pyroptosis and inflammation.Conclusion : Our findings suggest that pyroptosis might be playing an important role in the development of liver injury induced by CS. Overactivated ER stress response, followed by activation of the ATF6-CHOP signaling pathway, might be a novel molecular mechanism involved in CS-induced pyroptosis of liver tissue and cells.
Inhibition of Morusin From Mulberry Branches As An Agro-Waste to MDA-MB-453 and HCT116 Cancer Cells By Inducing Cell Apoptosis and Disturbing The Cell Cycle
Authors: Zhong Zhi-Hao , Zhang Yu-Qing 
Published: February 2021

Abstract
Mulberry tree branches are one of the largest agro-wastes produced in silk industry. How to make full use of this waste has always been one of the most important issues for the silk industry and even the entire biological industry. The paper has first reported that the inhibition of morusin recovered from mulberry branch barks, a prenylated flavonoid, on 20 kinds of tumour cells, of which the IC50 values of the 80% cells reaches about 15 µM. Second, effects on the proliferation, invasion and apoptosis of two cancer cells were investigated in detail. The experimental results showed that the apoptotic ratio of the high concentration was 77.73% in MDA-MB-453 cells. Western blotting displayed that morusin upregulated E-cadherin and downregulated vimentin and N-cadherin in a dose-dependent manner, and thus reversed epithelial-mesenchymal transition. It could upregulate cleaved Caspase-3 and Bax and downregulate Caspase-3 and Bcl-2, which indicate that the cell apoptosis is induced by morusin. These cancer cells, MDA-MB-453, were blocked in G2 phase, and HCT116 were arrested in S phase when treated with morusin, which is possible that the cell cycle is disturbed. Therefore, morusin could inhibit cancer migration and growth and promote cancer cell apoptosis.
A Proteogenomic Atlas of Clear Cell Renal Cell Carcinoma in a Chinese Population
Authors: Ding Chen , Qu Yuan-Yuan , Feng Jinwen , Wu Xiaohui , Bai Lin , Xu Wenhao , Zhu Lingli , Liu Yang , Xu Fujiang , Zhang Xuan , Yang Guojian , Lv Jiacheng , Chen Xiuping , Shi Guohai , Wang Hongkai , Cao Dalong , Xiang Hang , Li Lingling , Tan Subei , Gan Hualei , Sun Menghong , Zhang Hailiang , Zhao Jian-Yuan , Ye Dingwei 
Published: February 2021

Abstract
Renal cell carcinoma (RCC) is among the top 10 malignant carcinomas1. Clear cell (cc)RCC, accounting for ~ 75% of RCC cases, is an aggressive histological RCC subtype. In the last decade, large-scale multiomics studies have profoundly enhanced our understanding of this disease2,3. However, despite the differences of genomic alterations between Western and Eastern ccRCC4,5, these studies mostly focused on patients in Western populations. Here we conducted a comprehensive proteogenomic analysis of 232 tumor and adjacent non-tumor tissue pairs from Chinese ccRCC patients. Genomic analysis revealed unique genetic features of Chinese ccRCC and distinct mutation patterns associated with copy number alterations. Based on proteomic profiles, ccRCC showed extensive metabolic dysregulation, especially in one-carbon metabolism. We classified ccRCC into three subtypes (GP1–3), among which the most aggressive GP1 exhibited dominant immune response, metastasis, and metabolic imbalance, linking the proteomic features, genomic alterations, and clinical outcomes of ccRCC. Nicotinamide N-methyltransferase (NNMT) and NNMT mediated protein homocysteinylation were identified as a poor prognosis indicator and a drug target for GP1, respectively. We demonstrated that NNMT induces DNA-dependent protein kinase catalytic subunit (DNA-PKcs) homocysteinylation, increases DNA repair, and promotes tumor growth in ccRCC. Treatment of N-acetyl-cysteine (NAC), an inhibitor of homocysteinylation, markedly reduced the NNMT overexpression induced radioresistance of tumor cells. This study provided valuable insights into the biological underpinnings and prognosis assessment of ccRCC, revealing a targetable metabolic vulnerability.
A web-based survey of contact lens-related adverse events among Japanese female population: Are decorative contact lenses dangerous?
Authors: Ono Koichi , Murakami Akira , Haishima Yuji 
Published: February 2021

Abstract
Objectives:To compare the frequency of contact lens (CL)-related complications and to identify the risk factors for transparent and decorative CL users. Methods:A web-based, cross-sectional, observational survey of adverse events related to CL use was performed. The frequencies of complications were compared between two groups. Logistic regression analyses were performed to calculate an odds ratio (OR) for each metric. Age, replacement schedule, total experience, daily wear time, location of purchase, stacking of CLs, CL exchange with friends, compliance to hygiene procedure, replacement of CLs at intervals longer than recommended, and CL wear overnight were considered as confounders. Results:A total of 3,803 Japanese females were surveyed. The frequency of adverse events was 33.35% (95%CI: 31.27%-35.43%) and 35.73% (95%CI: 33.53%-37.98%) for transparent and decorative CLs, respectively. In a multivariate model, statistically significant factors associated with complications included the following: quarterly schedule lenses, replacement of CLs at intervals longer than recommended, compliance to hygiene procedure, CL wear overnight, CL purchase at physical shops and on the internet, and longer daily wearing time.Conclusions:Most of the risk-increasing behaviors are preventable. The role of public health ophthalmology is to increase awareness and to improve CL use behaviors.
Optimized Administration of Voriconazole and Therapeutic Drug Monitoring in Children and Adolescents: A Single-Centre Retrospective Experience from China
Authors: Zhao Yi-Chang , Zou Yang , Tang Dan , Xiao Chen-Lin , Xiao Yi-Wen , Wang Feng , Zhang Bi-Kui , Xiang Da-Xiong , Yu Feng , Yan Miao 
Published: February 2021

Abstract
Background

Voriconazole (VRC) is a triazole anti-fungal agent and a first-line treatment for invasive fungal infection (IFI) generally. The purpose of our study was performed to explore the factors affecting voriconazole trough concentration (Ctrough) and to show VRC dose adjustment experience in children.
Methods

The demographic information, concentration data, CYP2C19 genotypes and clinical outcomes of eligible children from January 1th, 2016 to December 31th, 2018 were collected. Factors affecting the voriconazole trough concentration were statistically analyzed.
Results

A total of 145 trough concentrations in 94 patients were included in this study, 54.5% of which achieved the target concentrations; however, 35.9% and 9.6% of which were sub-therapeutic and super-therapeutic post-multiple dosing. For children ≤ 2, 2–6, 6–12, and 12–18 years, the median VRC maintenance doses of 5.7, 6.7, 5.0 and 3.3 mg/kg twice daily respectively had been required in order to achieve therapeutic level (P < 0.001). Co-administration of proton pump inhibitors affected VRC target trough concentration significantly (P = 0.001).
Conclusion

Younger pediatric patients might need a higher dosage regime to achieve therapeutic trough concentration. In order to ensure the effectiveness and safety of voriconazole in children, early and repeat monitoring of voriconazole is a powerful tool.
Therapeutic method for early-stage second primary non-small lung cancer: analysis of a population-based database
Authors: Chen Congcong , Wu Zixiang , Wu Ziheng , Wu Chuanqiang , Wang Qi , Zhan Tianwei , Dong Lingjun , Fang Shuai , Wu Ming 
Published: February 2021

Abstract
Background Early-stage non-small lung cancer patients may survive long enough to develop second primary lung cancers. However, few studies have accurately described the therapeutic method, evaluation or prognostic factors for long-term survival in this complex clinical scenario.Methods Patients who had first and second primary non-small lung cancer in the Surveillance, Epidemiology, and End Results database between 2004 and 2015 were evaluated. The patients were included when their tumors were pathologically diagnosed as non-small lung cancer and in the early-stage (less than 3 cm and with no lymph node metastasis). Therapeutic methods were categorized as lobectomy, sublobectomy or no surgery. The influence of different therapeutic methods on the overall survival rate was compared. Results For the first primary tumor, patients who underwent lobectomy achieved superior survival benefits compared with patients who underwent sublobectomy. For the second primary tumor, long-term survival was similar in patients who underwent lobectomy and those who underwent sublobectomy treatment. The multivariate analysis indicated that age, disease-free time interval, sex, first and second type of surgery were independent prognostic factors for long-term survival. Our results showed that the 5-year overall survival rate was 91.9% when the disease-free interval exceeded 24 months.Conclusion Lobectomy for the first primary tumor followed by sublobectomy for the second primary tumor may be a beneficial therapeutic method for patients. If the disease-free interval exceeds 24 months, the second primary tumor will have no influence on the natural course for patients diagnosed with a first primary non-small lung cancer.
Annonaceous acetogenins mimic AA005 targets mitochondrial trifunctional enzyme a subunit to treat obesity
Authors: Wang Li-Shun , Han Bing , Li Zhan-Ming , Mao Yu-Qin , Zhang Shi-Long , Huang Li-Ying , Wu Zhao-Xia , Chen Hui-Ling , Kong Chao-Yue , Zhang Zheng-Yan , Yao Zhu-Jun , Chen Guoqiang 
Published: February 2021

Abstract
Obesity and related disease is a serious threat to people's health. However, currently available anti-obesity drugs couldn’t fully meet the clinic needs. We found annonaceous acetogenin mimic AA005 treatment could resist obesity in diet-induced obese mice and leptin-deficient (ob/ob) mice at non-cytotoxic dose. Then, with a bait of biotin labeled AA005, 44 proteins were captured as AA005 binding proteins using chemical proteomics. Alpha subunit of mitochondrial trifunctional protein (HADHA) showed the strongest affinity and its knockdown suppressed the fat accumulation in 3T3-L1 cells, identical to the effect of AA005. Pharmacokinetic analysis indicated that AA005 were mainly distributed in liver and fat tissue, and thus we constructed tissue-specific HADHA knockout mice of liver and fat. The obesities induced by high fat diet in these mice were significantly inhibited. Intriguingly, elevating energy expenditure and activated thermogenesis pathway were detected in AA005-treated as well as HADHA knockout mice. Thus, AA005 targeting HADHA may serve as novel therapeutic strategy for obesity.