bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antenatal Emergency Department and Inpatient Use Among Massachusetts Deaf and Hard of Hearing Women: A Retrospective Cohort Study
Authors: MCKEE Michael M. , ZHANG Jianying , AKOBIRSHOEV Ilhom , MCKEE Kimberly , MITRA Monika 
Published: December 2021

Abstract
Background
Hearing loss is increasingly prevalent among younger adults and significantly impacts health and health care use. Deaf and hard of hearing (DHH) women have a significantly higher risk of chronic diseases, pregnancy complications and adverse birth outcomes compared to hearing women. The mechanisms, including health care utilization patterns during the perinatal period, remains not well understood.
Methods
We conducted a retrospective cohort study design to analyze 2002–2013 Massachusetts Pregnancy to Early Life Longitudinal data to compare antenatal inpatient and emergency department use between DHH (N=925) and hearing (N=2895) women with singleton deliveries. Matching was done based on delivery year, age at delivery and birth parity in 1:3 case-control ratio. Demographic, socioeconomic, clinical and hospital characteristics were first compared for DHH mothers and the matched control group using chi-squared tests and t-tests. Multivariable models were adjusted for socio-demographic and clinical characteristics.
Results
Among DHH women (N=925), 49% had at least one emergency department visit, 19% had an observational stay, and 14% had a non-delivery hospital stay compared to 26%, 14%, and 6%, respectively among hearing women (N=2895) during the antenatal period (all p<0.001). The risk of non-delivery emergency department visits (RR 1.58; p<0.001) and inpatient stays (RR 1.89; p<0.001) remained higher among DHH women compared to hearing women even after adjustment for relevant variables. Having four or more emergency department visits (7% vs. 2%), two or more observational stays (7% vs. 3%) and two or more non-delivery hospital stays (4% vs. 0.4%) prior to delivery were more common among pregnant DHH women compared to their controls (all P<0.001).
Conclusion(s):
The study findings demonstrate that DHH women during the use emergency departments and inpatient services at a significantly higher rate than their hearing controls during the antenatal period. A systematic investigation of the mechanisms for these findings, including healthcare-, patient- and provider-based factors, are needed. Health care providers should be aware of potentially heightened risk for pregnancy and birth complications among DHH women which are in part due to persistent social and healthcare barriers, including communication and language.
A Practical Triage and Risk Scoring for Prediction of Early Mortality in Polytrauma Patients: GAS-TRS
Authors: Xie Tian , Wang Shikai , Du Nan , Huang Qunxing , Wu Jianguo , Mo Xiaoqiao , Zhang Xiangda , Tu Jiayuan , Cheng Xinsheng 
Published: December 2021

Abstract
Background Accurate evaluation of mortality risk in polytrauma patients is crucial for guiding the precision treatment strategy. There are few scales designed to provide an early assessment of mortality risk. However, the complexity of available scoring systems limits their application in pre-hospital care. Here, we established a GAS-TRS system to estimate the risk of early death for individual polytrauma patients and assess the early mortality risk in the individual patient.Methods We performed a secondary analysis from public Database. RCS and Multivariate Logistic regression analyses were used to screen potential prognostic factors for nomogram model. The VIF method examined multicollinearity, and VIF ≥ 5 suggested multicollinearity in this model. CMA was used to characterize the causality relationship in nomogram model. A four-layer back-propagation artificial neural network (BP-ANN) model was built by neuralnet package on R software. AUC of ROC analysis or F1 score were used to analyze the quality of predictive performance of GAS-TRS system. DCA and precision-recall curves were used to make up for the limitations of ROC curves.Results A total of 2406 patients were included in this analysis. Logistic regression analysis predicted four independent risk factors for nomogram model, including age (OR=1.03, 95%CI:1.02~1.03), GCS (OR=0.83, 95%CI:0.79~0.86), BE (OR=0.95, 95%CI:0.91~0.99) and serum lactic acid (OR=1.30, 95%CI:1.20~1.41) with an AUC of 0.88. Causal mediation analysis performed the mediation effect that lactate, age and BE accounted for 1.7%,0.7% and 3.0% indirect effect.The calibration curve showed model has good highly consistent with actual condition after bootstrapping. DCA showed the net benefit probability was between 2% and 85% and could bring more benefits for predicting early mortality.Then the input neurons were selected step by step in BP-ANN model. An optimal BP-ANN with an AUC of 0.91and AUPRC of 0.79 was established.Conclusion We established a GAS-TRS predictive system which includes a quick prognostic nomogram model and a precise BP-ANN model to evaluate early mortality within 72 hours for polytrauma patients. This scoring system might be practical and more efficient in identifying high-risk polytrauma patients. Moreover, this system may also guide triaging and precise initial individual treatment strategy for pre-hospital medical personnel.
Comparison of Diathermic high-frequency capsulorhexis and Femtosecond laser-assisted capsulorrhexis in white cataract surgery
Authors: Fan Licheng , Liu Liansheng , Zeng Yanfeng , Tian Haiyan 
Published: December 2021

Abstract
Aim: To compare the integrity, roundness and diameter of the capsulotomy in the white cataract between femtosecond laser assisted capsulotomy and high-frequency capsulorhexis.Methods: The prospective study included 32 patients with white-nuclear cataracts, 16 of whom underwent femtosecond capsulorhexis and another 16 underwent diathermic high-frequency capsulorhexis. The integrity roundness and diameter of the capsulorhexis post-operation were compared.Results: Femtosecond group obtained 6 cases(37.5%)of complete capsular. No anterior capsule tear occurred. Diathermy high-frequency achieved continuous complete capsulor in 3 eyes(18.75%) and anterior capsule was teared in 13 eyes(81.25%). The femtosecond group has a mean capsular diameter of 5.4 mm, and the diathermic high-frequency group has an average capsular diameter of 6 mm. No posterior capsule tear occurred in both surgical procedures. Conclusions: Femtosecond laser-assisted cataract surgery can achieve high capsulor integrity and stable capsulorhexis diameter. Compared with the diathermic high-frequency capsulorhexis, the frequency of injecting the viscoelastic agent was reduced, and the tear of the capsule was less likely to occur. The continuous complete capsular(CCC) by femtosecond laser is more round, more complete ,more controllable and smoother than diathermy high-frequency(DHC).
How the Metabolic Phenotype in Adulthood Is Affected by Long-Lasting Immunological Trajectories Since Adolescence
Authors: Barroso Isaac , Guimarães João Tiago , Craveiro Vanda , Severo Milton , Ramos Elisabete 
Published: December 2021

Abstract
A close relationship between immune and metabolic systems has been perceived in the recent past. We aimed to assess whether the immunological trajectories of circulating white blood cells (WBC) started in adolescence, affects the metabolic phenotype in adulthood. We used data from 1183 participants of the population-based EPITeen cohort, evaluated at 13, 17, 21, 24 and 27 years of age. The Immunological trajectories from 13 to 27 years old were identified by mixed-effects models, being their association with metabolic features at 27 years old measured by logistic regression. The Higher Inflammatory Activation trajectory (HIA trajectory) had the highest percentage of individuals with metabolic syndrome, while Lowest Levels of WBC trajectory (LLWBC trajectory) showed the lowest percentage. Participants with HIA trajectory had significantly higher triglycerides, waist circumference, serum uric acid and BMI. After adjustment for sex and sports practice and hs-CRP, the odds of having one or more metabolic features in adulthood was significantly lower in LLWBC trajectory. Individuals with immunological trajectories of WBC linked with a pattern of higher immune activation showed a less favourable metabolic profile, while those with the lowest levels of WBC were less likely to have metabolic risk factors in adulthood.
Yearly Trends In The Prevalence of Ocular Traumas And The Effects of Covid-19: A Nationwide Population-Based Study of Korean Data
Authors: Whang Woong-Joo , Kwon Jin-woo 
Published: December 2021

Abstract
We identify the yearly trends in the prevalence of various ocular traumas in the Republic of Korea, and the effects of Covid-19. We recorded the yearly numbers of patients diagnosed with hyphema and those who underwent open reduction surgery because of orbital blowout fracture (BOF), primary closure of the cornea or sclera (PCCS), or intraocular foreign body (IOFB) removal. The incidence of closed globe injuries has decreased significantly over the past 10 years, while that of open globe injuries has not. The patients aged ≥ 60 years showed a significant increase in BOF surgery. Regarding the trend in PCCS, while other age groups showed significant decreases, the population aged ≥ 60 years did not. In addition, the population aged ≥ 60 years showed a significant increase in the incidence of IOFB. When the 2020 data were compared with those of 2019, hyphema evidenced the largest decrease (27.02%), especially in those aged < 20 years (54.33%). In terms of both BOF and IOFB, those aged < 20 years showed the largest decreases (28.85% and 47.93% respectively). In the Covid-19 era, ocular trauma fell markedly in those aged < 20 years, perhaps because of school closures and reductions in outdoor activities.
Screening of Osteoarthritis-Related Genes and Function Determination Based on Bioinformatics Tools
Authors: Fu Zheng , Jiang Weiqian , Yan Wenlong , Xie Fei , Chen Yu , Zhang Jian 
Published: December 2021

Abstract
Background:Osteoarthritis(OA), commonly seen in the middle-aged and elderly population, imposes a heavy burden on patients from the clinical, humanistic and economic aspects. Our work aims at the discovery of early diagnostic and therapeutic targets for OA and new candidate biomarkers for experimental studies on OA via bioinformatics analysis.Methods:The dataset GSE114007 was downloaded from GEO to identify differentially expressed genes(DEGs) in R using 3 different algorithms. Overlapping DEGs were subject to GO and KEGG pathway enrichment analysis and functional annotation. Following the identification of DEGs, a protein-protein interaction(PPI) network was established and imported into Cytoscape to screen for hubgenes. The expression of each hubgene was verified in two other datasets and create miRNA-mRNA regulatory networks.Results:174 upregulated genes and 117 downregulated genes were identified among the overlapping DEGs. According to the results of GO enrichment analysis,MF enrichment was basically found in ECM degradation and collagen breakdown; enrichment was also present in the development, ossification, and differentiation of cells. The KEGG pathway enrichment analysis suggested significant enrichment in such pathways as PI3K-AKT, P53, TNF, and FoxO. 23 hubgenes were obtained from the PPI network, and 11 genes were identified as DEGs through verification. 8 genes were used for the establishment of miRNA-mRNA regulatory networks.Conclusion:OA-related genes, proteins, pathways and miRNAs that were identified through bioinformatics analysis may provide a reference for the discovery of early diagnostic and therapeutic targets for OA, as well as candidate biomarkers for experimental studies on OA.
Age-Dependent Aggregation of α-Synuclein in the Nervous System of Gut-Brain Axis is Associated with Caspase-1 Activation
Authors: Hu Qi , Hong Mei , Huang Mengyang , Gong Quan , Zhang Xiaofan , Uversky Vladimir N. , Pan-Montojo Francisco , Huang Teng , Zhou Honglian , Zhu Suiqiang 
Published: December 2021

Abstract
α-Synuclein (α-Syn) plays a key role in the development of Parkinson’ desease (PD). As aging is acknowledged to be the greatest risk factor for PD, here we investigated α-Syn expression in the ileum, thoracic spinal cord, and midbrain of young (1-month-old), middle-aged (6-, 12-month-old) to old (18-month-old) mice. We demonstrated that both the levels of α-Syn monomers, oligomers and ratios of oligomers to monomers were increased with aging in the ileum, thoracic spinal cord, and midbrain. Whereas, the expression of tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine synthesis, was decreased with aging in the midbrain. We failed to find corresponding α-Syn mRNA increase with aging. However, we found an increased expression of caspase-1 in the ileum, thoracic spinal cord, and midbrain. A specific caspase-1 inhibitor VX765 significantly reduced levels of both the α-Syn monomers and oligomers triggered by the rotenone in vitro. Taken together, the increase in α-Syn aggregation with aging might not occur first in the gut, but simultaneously in the nervous system of gut-brain axis.. The mechanism of the age-dependent aggregation of α-Syn in nervous system is likely triggered by the aging-related caspase-1 activation.
Prevalence And Definitions of Polypharmacy: A Systematic Review And Meta-Analysis
Authors: Delara Mahin , Murray Lauren , Jafari Behnaz , Bahji Anees , Goodarzi Zahra , Kirkham Julia , Seitz Dallas P. 
Published: December 2021

Abstract
INTRODUCTION: Polypharmacy is common associated with several adverse health outcomes. There are currently no systematic reviews or meta-analyses on the prevalence of polypharmacy and associated factors. We aimed to identify population-based observational studies reporting on the prevalence of polypharmacy and factors associated with polypharmacy. METHODS: MEDLINE, EMBASE, and Cochrane databases with no restriction on date. Population-based observational studies with cross-sectional, case-control, or cohort designs using administrative databases or registries to define or measure polypharmacy among individuals over 19. Using a standardized form, two reviewers independently extracted study characteristics, a crude prevalence rate of polypharmacy and its standard error with 95% confidence intervals (CIs). The risk of bias and quality of studies was assessed using the Newcastle-Ottawa Scale. The main outcome was the prevalence of polypharmacy and factors associated with polypharmacy. Using a random-effects model, pooled prevalence estimates with 95% CI was reported. Subgroup analysis was performed if significant heterogeneity was explored. Meta-regression analysis was conducted to predict polypharmacy prevalence.RESULTS: 106 full-text articles were identifies using 21 unique terms with 138 descriptive definitions of polypharmacy. The pooled estimated prevalence polypharmacy in studies reporting all medication classes was 37% (95% CI: 31%-43%). Differences in polypharmacy prevalence were reported for studies using different numerical threshold and polypharmacy was also associated with study year in meta-regression. Sex, study geography, study design and study setting were not associated with differences in polypharmacy prevalence. DISCUSSION: Our review highlights that polypharmacy is common particularly among older adults and those in inpatient settings. A variety of definitions are used to define polypharmacy and differences in polypharmacy definitions may have implications for understanding the burden or polypharmacy and outcomes associated with polypharmacy. CONCLUSIONS AND IMPLICATIONS: Clinicians should be aware of the common occurrence of polypharmacy in all populations and undertake efforts to minimize inappropriate polypharmacy whenever possible.
Blood Glucose Level and Risk of Multiple HPV Infections in Chinese Patients of a Pathologically Confirmed HSIL with HPV Effection: A Restrospective Cross-Sectional Study
Authors: zhou jie , Wei Xiang cai , Xu Hong yan , Hu Hong bo , Ma Zhan zhong , Li Fan xiang , Zhou Wei juan , Chen Ye , Liu Zhen 
Published: December 2021

Abstract
BACKGROUND

Apart from the controversy of high glycemic index and glycemic load associated with precancerous cervical lesions, few studies have examined the impact of fasting blood glucose levels on HPV multiple infections. In a population of HPV-positive women with high-grade cervical lesions, we conducted the current study to appraisal the relationship between blood glucose levels and multiple HPV infections.
METHODS

The present study was a cross-sectional correlative study. A total of 629 participants with a pathologically confirmed HSIL with HPV infection were included from a hospital in China between 2018-1-1 and 2019-12-31. The target independent variable and the dependent variable were glucose level at baseline and multiplicity of HPV respectively. The ratio (OR) and 95% confidence intervals (95% CIs) for the risk of multiple infections were estimated using logistic regression analysis.
RESULTS

The average age of 560 participants was 44.63 ± 10.61 years old, Result of fully-adjusted binary logistic regression showed glucose was negatively associated with risk of event of multiple infection after adjusting confounders (Odds ratio (OR)=0.84, 95%CI(0.57-1.22). Non-linear relationship was detected between glucose and multiplicity of HPV, with point at 5.4. After adjusting for the full range of variables, the effect sizes and confidence intervals for the left and right sides of the inflection points were 0.379 (0.196, 0.732) and 5.083 (1.592, 16.229), respectively.
CONCLUSION

In this cross-sectional study, both hypoglycemia and hyperglycemia predicted an increased risk of multiple infections, demonstrating a U-shaped association between blood glucose levels and HPV multiple infections.
Analysis of the Potential Role of Key Genes in Atrial Fibrillation Based on Bioinformatics Approach
Authors: He Dan , Ruan Zhong-bao , Song Gui-xian , Chen Ge-cai , Zhu Li , Wang Fei , Wang Mei-xiang 
Published: December 2021

Abstract
Objective: Our study aims to explore the key differentially expressed genes (DEGs) that may serve as potential biomarkers for the diagnosis and treatment of atrial fibrillation (AF) using bioinformatics tools.Methods: Microarray datasets of GSE31821 and GSE79768 were downloaded from Gene Expression Synthesis (GEO) database. DEGs were analyzed after merging all microarray data and adjusting batch effect. The screened DEGs were further used for Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis. Protein-protein interaction (PPI) network was constructed using the STRING database，and PPI nodes were counted by R software. Finally, combined with the above important bioinformatics information, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect some DEGs in the tissues of patients with AF.Results:114 DEGs (|log2 FC|≥0.5) were identified in the AF group compared with the control group. Combining DEGs, enrichment analysis and PPI results, CXCL10, TLR7, DDX58, CCR2, RSAD2, KIT, LYN, and CXCL11 were identified as potential key genes. The expression of two key genes (RSAD2 and CXCL11) was also verified by qRT-PCR in the tissues of AF patients, illustrating the reliability and biomarker potential of the key genes.Conclusion: 8 potential key genes may play an important role in the development of AF, and they may serve as potential biomarkers for the diagnosis and treatment of AF.
The Effect of Methyltransferase NSUN2 in Stroke
Authors: Ni Xuqing , Liu Xia , Yao Xinyu , Li Shan , Zhu Yurun , Zhu Ye , Mo Yunchang , Dai Qinxue , Wang Junlu 
Published: December 2021

Abstract
Stroke is one of the most important diseases that seriously threaten the health and public health of elderly patients.NSUN2 refers to the predominant methyltransferase for RNA m5C methylation, contributing to increased RNA stability, translocation and translation, and playing an important role in the physiopathology. However, there is insignificant progress on the biological functions and mechanisms of NSUN2 in cerebral ischemia-reperfusion injury. Here, C57BL/6 mice were employed to establish a middle cerebral artery ischemia-reperfusion injury model (MCAO) and found to significantly increase in NSUN2 protein and mRNA expression levels by Western blotting and qRT-PCR. Subsequently, NSUN2 knockout mice were exploited to build the MCAO model. This study reported that knockout of NSUN2 significantly aggravated brain infarct size and behavioral scores, while reducing 7-day postoperative survival and increasing neuronal apoptosis and injury in MCAO mice. According to the investigation of Western blotting results, decreased PI3K/AKT, ICAM-1 and Bcl-2 protein expressions and increased apoptosis-related protein (Caspase-3/Bax) were found. Overall, this study suggested that NSUN2 may affect cerebral ischemia-reperfusion injury via PI3K/AKT signaling channel and ICAM-1 protein regulation of apoptosis.
Missing Samples Estimation of Synthetic ECG Signals by FCM-based Adaptive Neuro-Fuzzy Inference System (FCMANFIS)
Authors: dabiri asghar , Dabanloo Nader Jafarnia , Rahatabad Fereidoon Nooshirvan , Maghooli Keivan 
Published: December 2021

Abstract
This paper presents estimation of missed samples recovery of Synthetic electrocardiography (ECG) signals by an ANFIS (Adaptive neuro-fuzzy inference system) method. After designing the ANFIS model using FCM (Fuzzy C Means) clustering method. In MATLAB’s standard library for ANFIS, only least-square-estimation and the back-propagation algorithms are used for tuning membership functions and generation of fis (fuzzy inference system) file, but at current work we have used FCM method that shows better result. Root mean square error (difference of the reference input and the generated data by ANFIS) for the three synthetic data cases are: a. Train data: RMSE = 1.7112e-5b. Test data: RMSE = 5.184e-3c. All data: RMSE = 2.2663e-3
Exploring New Targets and Publication Tendency of Triple Negative Breast Cancer: A 20-Year Bibliometric Analysis
Authors: Sheng Xiaonan , Dai Huijuan , Song Yonggang , Ma Xueyun 
Published: December 2021

Abstract
Background: Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and it lacks an efficient target treatment. Here, we aimed to gain knowledge on the development of TNBC research and explore potential treatment strategies.Methods: We analyzed 14,389 publications on TNBC from the Web of Science (WOS) over the past 20 years, from 2000 to 2020, using bibliometric methods. We evaluated the publication tendency of TNBC and the contributions of different countries. Institutions and journals with the highest number of TNBC publications were screened. Finally, the research focus of the TNBC publications were also analyzed.Results: TNBC publications have significantly increased in the past 20 years, with elevated relative research interest (RRI). The USA has the most TNBC-related publications with high quality, and China is the country with the most rapid growth tendency in TNBC publications. The University of Texas System is the institution with the most TNBC publications. Breast Cancer Research and Treatment is the journal that published the most TNBC-related publications. The top 30 publications with high citations are also listed. The researches focusing on TNBC in the past 20 years were separated into four main clusters: tumor biology, TNBC therapies, treatment sensitivity, and gene mutations. The research focus in TNBC ranked by appearing years reflects the development of TNBC treatment strategy, showing that targeting tumor immunity is now the main focus in TNBC research. Conclusions: Using bibliometric analysis, we initially revealed the increasing interest in TNBC research and summarized the publication tendency of TNBC. We also reported focused topics screened from publications in the past 20 years, indicating the main problems and research objectives of TNBC for the first time. Immune-related topics are becoming the focus of TNBC research.