bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovering Knowledge and Cognitive Based Drivers for SMEs Internationalization
Authors: Alinasab Jamshid , Mirahmadi Seid Mohammad Reza , Ghorbani Hassan , Caputo Francesco 
Published: July 2021

AbstractInternationalisation begins with companies’ decision to enter global markets to develop knowledge and experience as key competitive factors in the global economy, which has been the subject of much empirical research. Decision-making, knowledge management, and effective internationalisation have become key strategic tools for all companies, especially for small- and medium-sized enterprises (SMEs). This study wants to provide a framework for SMEs internationalisation based on the real options theory, (ROT) as a knowledge driver method. For this purpose, the effective factors for internationalisation were identified by reviewing the literature on the subject and the internal and external backgrounds of the subject. Then, main and sub-factors were prioritised by the fuzzy analytic hierarchy process (AHP) method. The statistical population consisted of senior managers, business managers of SMEs in Isfahan, Iran, who were eligible to enter this study. Twenty-six experts participated in this study by judgmental non-random sampling method. A fuzzy AHP questionnaire was prepared in the form of 19 sub-factors and 7 main factors. The components of each factor in each group were also ranked by experts, and their weights were obtained. Next, according to the ROT strategies which have 5 options, an alignment matrix was used to align the factors affecting the decision with the strategies. After answering the research question, the option that had a higher mode was consideredAS; then, this score was multiplied by the weight obtained in the previous step and theTASwas obtained. Finally, strategies were classified as appropriate, need further investigation, and inappropriate.
The Sibling Distribution for Multivariate Life Time Data
Authors: Helgøy Ingvild M. , Skaug Hans J. 
Published: July 2021

AbstractA flexible class of multivariate distributions for continuous lifetimes is proposed. The distribution is defined in terms of the age-at-death of m siblings. The expression for the joint density is derived using classical results from mathematical demography. The parameters of the distribution are the age-specific birth and death rates, in addition to a vector of relative death times for the m siblings. For the case of constant birth and death rates we are able to derive an explicit expression for the bivariate sibling density, which is proven to be MTP2, and hence has positive dependence. Further, we show that a special case of the sibling distribution belongs to the Block-Basu class of multivariate distribution. In the general case, with age-dependent birth and death rates, evaluation of the density involves numerical integration, but is still feasible.
Lifetime predictions of non-ionic and ionic biopolymers: kinetic studies by non-isothermal thermogravimetric analysis
Authors: Calvino Martina Maria , Lisuzzo Lorenzo , Cavallaro Giuseppe , Lazzara Giuseppe , Milioto Stefana 
Published: July 2021

AbstractIn this paper, films based on sustainable polymers with variable charge have been investigated by non-isothermal thermogravimetry in order to predict their lifetime, which is a key parameter for their potential use in numerous technological and biomedical applications. Specifically, chitosan has been selected as positively charged biopolymer, while alginate has been chosen as negatively charged biopolymer. Among non-ionic polymers, methylcellulose has been investigated. Thermogravimetric measurements at variable heating rates (5, 10, 15 and 20 °C min−1) have been performed for all the polymers to study their degradation kinetics by using isoconversional procedures combined with ‘Master plot’ analyses. Both integral (KAS and Starink methods) and differential (Friedman method) isoconversional procedures have shown that chitosan possesses the highest energetic barrier to decomposition. Based on the Master plot analysis, the decomposition of ionic polymers can be described by the R2 kinetic model (contracted cylindrical geometry), while the degradation of methylcellulose reflects the D2 mechanism (two-dimensional diffusion). The determination of both the decomposition mechanism and the kinetic parameters (activation energy and pre-exponential factor) has been used to determine the decay time functions of the several biopolymers. The obtained insights can be helpful for the development of durable films based on sustainable polymers with variable electrostatic characteristics.
Graphical abstract
Evolution of forced magnetohydrodynamic waves in a stratified fluid
Authors: Sreenivasan Binod , Maurya Gaurav 
Published: July 2021

The evolution of a buoyancy disturbance in a stratified incompressible fluid permeated by a uniform vertical magnetic field is investigated. Two regimes are considered in the absence of background rotation – that of strong stratification, where the internal gravity wave frequency

$\omega _A$

is much higher in magnitude than the magnetic (Alfvén) wave frequency

$\omega _M$

, and that of strong magnetic field, where

$\omega _M$

is dominant. For small but finite magnetic diffusion, perturbations that initially lie in the strong-field regime are shown to cross over to the regime of strong stratification, so that small-scale motions may exist as damped internal gravity waves at large times. The induced magnetic field propagates as damped Alfvén waves for a much longer time than the velocity before undergoing the above transition. With strong rotation, the unstably stratified system that satisfies the inequality

$|\omega _C| > |\omega _M| \gg |\omega _A| \gg |\omega _\eta |$

, where

$\omega _C$

is the inertial wave frequency and

$\omega _\eta$

is the diffusion frequency, is of relevance to convection-driven dynamos. Here, a parameter space with

$|\omega _M/\omega _C| \sim 0.1$

is found wherein the flow intensity of the slow magnetic-Archimedean-Coriolis (MAC) waves is of the same order of magnitude as that of the fast MAC waves. Slow wave motions at horizontal length scales much smaller than the width of the fluid layer can therefore generate substantial helicity in rapidly rotating dynamos. The excitation of slow MAC waves at scales of

$\sim$

10 km in the Earth's core may play a crucial role in the generation of the axial dipole field.
Carbon isotope chemostratigraphy and sea-level history of the Hirnantian Stage (uppermost Ordovician) in the Oslo–Asker district, Norway
Authors: Calner Mikael , Bockelie Johan Fredrik , Rasmussen Christian M.Ø. , Calner Hanna , Lehnert Oliver , Joachimski Michael M. 
Published: July 2021

AbstractWe present a δ13Ccarb chemostratigraphy for the Late Ordovician Hirnantian Stage based on 208 whole-rock samples from six outcrops in the Oslo–Asker district, southern Norway. Our data include the Norwegian type section for the Hirnantian Stage and Ordovician–Silurian boundary at Hovedøya Island. The most complete record of the Hirnantian Isotope Carbon Excursion (HICE) is identified in a coastal exposure at Konglungø locality where the preserved part of the anomaly spans a c. 24 m thick, mixed carbonate–siliciclastic succession belonging to the upper Husbergøya, Langåra and Langøyene formations and where δ13Ccarb peak values reach c. +6 ‰. Almost the entire HICE occurs above beds containing the Hirnantia Fauna, suggesting a latest Hirnantian age for the peak of the excursion. The temporal development of the HICE in southern Norway is associated with substantial shallowing of depositional environments. Sedimentary facies and erosional unconformities suggest four inferably fourth-order glacio-eustatically controlled sea-level lowstands with successively increased exposure and erosion to the succession. The youngest erosional unconformity is related to the development of incised valleys and resulted in cut-out of at least the falling limb of the HICE throughout most of the Oslo–Asker district. The fill of the valleys contains the falling limb of the HICE, and the postglacial transgression therefore can be assigned to the latest part of the Hirnantian Age. We address the recent findings of the chitinozoan Belonechitina gamachiana in the study area and its relationship to the first occurrence of Hirnantia Fauna in the studied sections, challenging identification of the base of the Hirnantian Stage.
REGULATORY FIT EFFECTS ON THE ACQUISITION OF LEXICAL STRESS
Authors: Cho Minyoung 
Published: July 2021

AbstractHiggins’s (2000) regulatory fit theory proposes that a fit between one’s regulatory state and strategic means for reaching a goal increases motivational strength and engagement. This study investigates how regulatory fit affects the L2 acquisition of lexical stress in an authentic learning context. Ninety EFL students were assigned to either gain frame or loss frame conditions. They engaged in speech practice in which they mimicked a model speech in English to win a chance to enter a prize raffle. The reward system was framed differently in the two framing conditions, with the intention of eliciting the participants’ use of eager or vigilant strategies, thus creating fit and nonfit conditions. Acquisition of lexical stress was assessed using pre- and posttest scores. Multiple regression analysis showed no regulatory fit effects and no loss frame effects but did show a significant beneficial effect of the gain frame on the acquisition of lexical stress.
A parametric approach to the acquisition of syntax
Authors: SNYDER William 
Published: July 2021

AbstractThree case-studies, using longitudinal records of children's spontaneous speech, illustrate what happens when a child's syntax changes. The first, examining acquisition of English verb-particle constructions, shows a near-total absence of commission errors. The second, examining acquisition of prepositional questions in English or Spanish, shows that children (i) may go as long as 9 months producing both direct-object questions and declaratives with prepositional phrases, before first attempting a prepositional question; and (ii) at some point, abrubtly begin producing prepositional questions that are correctly formed for the target language. The third case study shows that in children acquiring English, the onset of verb-particle constructions occurs almost exactly when that child begins using novel noun-noun compounds. After a discussion of the implications for the nature of syntactic knowledge, and for the mechanisms by which it is acquired, two examples are presented of as-yet untested acquisitional predictions of parametric proposals in the syntax literature.
Morphodynamics of Barchan‐Barchan Interactions Investigated at the Grain Scale
Authors: Assis W. R. , Franklin E. M. 
Published: July 2021

AbstractCorridors of size‐selected crescent‐shaped dunes, known as barchans, are commonly found in water, air, and other planetary environments. The growth of barchans results from the interplay between a fluid flow and a granular bed, but their size regulation involves intricate exchanges between different barchans within a field. One size‐regulating mechanism is the binary interaction between nearby dunes, when two dunes exchange mass via the near flow field or by direct contact (collision). In a recent Letter (Assis & Franklin, 2020, https://doi.org/10.1029/2020GL089464), we identified five different patterns arising from binary interactions of subaqueous barchans and proposed classification maps. In this paper, we further inquire into binary exchanges by investigating the motion of individual grains while barchans interact with each other. The experiments were conducted in a water channel where the evolution of pairs of barchans in both aligned and off‐centered configurations was recorded by conventional and high‐speed cameras. Based on image processing, we obtained the morphology of dunes and motion of grains for all interaction patterns. We present the trajectories of individual grains, from which we show the origin and destination of moving grains, and their typical lengths and velocities. We also show that grains from the impacting dune spread with a diffusion‐like component over the target barchan, and we propose a diffusion length. Our results provide new insights into the size‐regulating mechanisms of barchans and barchanoid forms found on Earth and other planets.
Transcriptional response to host chemical cues underpins the expansion of host range in a fungal plant pathogen lineage
Authors: Kusch Stefan , Larrouy Justine , Ibrahim Heba M M , Mounichetty Shantala , Gasset Noémie , Navaud Olivier , Mbengue Malick , Zanchetta Catherine , Lopez-Roques Céline , Donnadieu Cécile , Godiard Laurence , Raffaele Sylvain 
Published: July 2021

Abstract
The host range of parasites is an important factor in assessing the dynamics of disease epidemics. The evolution of pathogens to accommodate new hosts may lead to host range expansion, a process the molecular bases of which are largely enigmatic. The fungus Sclerotinia sclerotiorum has been reported to parasitize more than 400 plant species from diverse eudicot families while its close relative, S. trifoliorum, is restricted to plants from the Fabaceae family. We analyzed S. sclerotiorum global transcriptome reprogramming on hosts from six botanical families and reveal a flexible, host-specific transcriptional program. We generated a chromosome-level genome assembly for S. trifoliorum and found near-complete gene space conservation in two representative strains of broad and narrow host range Sclerotinia species. However, S. trifoliorum showed increased sensitivity to the Brassicaceae defense compound camalexin. Comparative analyses revealed a lack of transcriptional response to camalexin in the S. trifoliorum strain and suggest that regulatory variation in detoxification and effector genes at the population level may associate with the genetic accommodation of Brassicaceae in the Sclerotinia host range. Our work proposes transcriptional plasticity and the co-existence of signatures for generalist and polyspecialist adaptive strategies in the genome of a plant pathogen.
Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride
Authors: Atriztán-Hernández Karina , Herrera-Estrella Alfredo 
Published: July 2021

Abstract
The capability to respond to wounding is a process shared by organisms of different kingdoms that can result in the regeneration of whole-body parts or lost structures or organs. Filamentous fungi constitute a rich food source that ensures survival and reproduction of their predators and are therefore continuously exposed to mechanical damage. Nevertheless, our understanding of how fungi respond to wounding and predators is scarce. Fungi like plants and animals respond to injury recognizing Damage- and Microbe-Associated Molecular Patterns (DAMPs/MAMPs) that activate Ca2+ and Mitogen-Activated Protein Kinase dependent signaling for the activation of defense mechanisms. During herbivory, plants, in addition to activating pathways related to injury, activate specific responses to combat their predators. Using a transcriptional approach, we studied the capacity of the filamentous fungus Trichoderma atroviride to activate specific responses to injury and attack by different arthropods. Attack by Drosophila melanogaster inhibited the transcriptional activation of genes required for hyphal regeneration, and the fungal innate immune and chemical defense responses. We also provide mechanistic insight of this inhibition involving components of the D. melanogaster salivary glands that repress the expression of a set of genes and block hyphal regeneration.
The rates of global bacterial and archaeal dispersal
Authors: Louca Stilianos 
Published: July 2021

Abstract
The phylogenetic resolution at which microorganisms display geographic endemism, the rates at which they disperse at global scales, and the role of humans on global microbial dispersal are largely unknown. Answering these questions is necessary for interpreting microbial biogeography, ecology, and macroevolution and for predicting the spread of emerging pathogenic strains. To resolve these questions, I analyzed the geographic and evolutionary relationships between 36,795 bacterial and archaeal (“prokaryotic”) genomes from ∼7000 locations around the world. I find clear signs of continental-scale endemism, including strong correlations between phylogenetic divergence and geographic distance. However, the phylogenetic scale at which endemism generally occurs is extremely small, and most “species” (defined by an average nucleotide identity ≥ 95%) and even closely related strains (average nucleotide identity ≥ 99.9%) are globally distributed. Human-associated lineages display faster dispersal rates than other terrestrial lineages; the average net distance between any two human-associated cell lineages diverging 50 years ago is roughly 580 km. These results suggest that many previously reported global-scale microbial biogeographical patterns are likely the result of recent or current environmental filtering rather than geographic endemism. For human-associated lineages, estimated transition rates between Europe and North America are particularly high, and much higher than for non-human associated terrestrial lineages, highlighting the role that human movement plays in global microbial dispersal. Dispersal was slowest for hot spring- and terrestrial subsurface-associated lineages, indicating that these environments may act as “isolated islands” of microbial evolution.
Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow
Authors: Li Fengchan , Yan Kunmin , Wu Lili , Zheng Zhong , Du Yun , Liu Ziting , Zhao Luyao , Li Wei , Sheng Yulan , Ren Lijie , Tang Chaojun , Zhu Li 
Published: July 2021

AbstractDisturbed blood flow (d-flow) has been known to induce changes of the cells in the arterial wall, increasing the risk of atherosclerosis. However, the heterogeneity of the vascular cell populations under d-flow remains less understood. To generate d-flow in vivo, partial carotid artery ligation (PCL) was performed. Seven days after ligation, single-cell RNA sequencing of nine left carotid arteries (LCA) from the PCL group (10,262 cells) or control group (14,580 cells) was applied and a single-cell atlas of gene expression was constructed. The integrated analysis identified 15 distinct carotid cell clusters, including 10 d-flow-relevant subpopulations. Among endothelial cells, at least four subpopulations were identified, including Klk8hi ECs, Lrp1hi ECs, Dkk2hi ECs, and Cd36hi ECs. Analysis of GSVA and single-cell trajectories indicated that the previously undescribed Dkk2hi ECs subpopulation was mechanosensitive and potentially transformed from Klk8hi ECs under d-flow. D-flow-induced Spp1hi VSMCs subpopulation that appeared to be endowed with osteoblast differentiation, suggesting a role in arterial stiffness. Among the infiltrating cell subpopulations, Trem2hi Mφ, Birc5hi Mφ, DCs, CD4+ T cells, CXCR6+ T cells, NK cells, and granulocytes were identified under d-flow. Of note, the novel Birc5hi Mφ was identified as a potential contributor to the accumulation of macrophages in atherosclerosis. Finally, Dkk2hi ECs, and Cd36hi ECs were also found in the proatherosclerotic area of the aorta where the d-flow occurs. In conclusion, we presented a comprehensive single-cell atlas of all cells in the carotid artery under d-flow, identified previously unrecognized cell subpopulations and their gene expression signatures, and suggested their specialized functions.
CD40 stimulation as a molecular adjuvant for cancer vaccines and other immunotherapies
Authors: Bullock Timothy N. J. 
Published: July 2021

AbstractThe substantial advances attained by checkpoint blockade immunotherapies have driven an expansion in the approaches used to promote T cell access to the tumor microenvironment to provide targets for checkpoint immunotherapy. Inherent in any T cell response to a tumor antigen is the capacity of dendritic cells to initiate and support such responses. Here, the rationale and early immunobiology of CD40 as a master regulator of dendritic cell activation is reviewed, with further contextualization and appreciation for the role of CD40 stimulation not only in cancer vaccines but also in other contemporary immune-oncology approaches.
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors: Mathieu Mathilde , Névo Nathalie , Jouve Mabel , Valenzuela José Ignacio , Maurin Mathieu , Verweij Frederik J. , Palmulli Roberta , Lankar Danielle , Dingli Florent , Loew Damarys , Rubinstein Eric , Boncompain Gaëlle , Perez Franck , Théry Clotilde 
Published: July 2021

AbstractDespite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and their secretion mechanisms are not fully characterized: how and to what extent EVs form as intraluminal vesicles of endocytic compartments (exosomes), or at the plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking of the EV markers CD9 and CD63 from the endoplasmic reticulum to their residency compartment, respectively PM and late endosomes. We observe transient co-localization at both places, before they finally segregate. CD9 and a mutant CD63 stabilized at the PM are more abundantly released in EVs than CD63. Thus, in HeLa cells, ectosomes are more prominent than exosomes. By comparative proteomic analysis and differential response to neutralization of endosomal pH, we identify a few surface proteins likely specific of either exosomes (LAMP1) or ectosomes (BSG, SLC3A2). Our work sets the path for molecular and functional discrimination of exosomes and small ectosomes in any cell type.
Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease
Authors: Ban Tatsuma , Kikuchi Masako , Sato Go R. , Manabe Akio , Tagata Noriko , Harita Kayo , Nishiyama Akira , Nishimura Kenichi , Yoshimi Ryusuke , Kirino Yohei , Yanai Hideyuki , Matsumoto Yoshiko , Suzuki Shuichi , Hihara Hiroe , Ito Masashi , Tsukahara Kappei , Yoshimatsu Kentaro , Yamamoto Tadashi , Taniguchi Tadatsugu , Nakajima Hideaki , Ito Shuichi , Tamura Tomohiko 
Published: July 2021

AbstractThe transcription factor IRF5 has been implicated as a therapeutic target for the autoimmune disease systemic lupus erythematosus (SLE). However, IRF5 activation status during the disease course and the effects of IRF5 inhibition after disease onset are unclear. Here, we show that SLE patients in both the active and remission phase have aberrant activation of IRF5 and interferon-stimulated genes. Partial inhibition of IRF5 is superior to full inhibition of type I interferon signaling in suppressing disease in a mouse model of SLE, possibly due to the function of IRF5 in oxidative phosphorylation. We further demonstrate that inhibition of IRF5 via conditional Irf5 deletion and a newly developed small-molecule inhibitor of IRF5 after disease onset suppresses disease progression and is effective for maintenance of remission in mice. These results suggest that IRF5 inhibition might overcome the limitations of current SLE therapies, thus promoting drug discovery research on IRF5 inhibitors.