bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations Between Periconceptional Lifestyle Behaviour Change and Adverse Pregnancy Outcomes
Authors: Maas Veronique Y.F. , Poels Marjolein , Ruiter Marije Lamain-de , Kwee Anneke , Bekker Mireille N. , Franx Arie , Koster Maria P.H. 
Published: February 2021

Abstract
Background
While the effect of prenatal exposure to unhealthy lifestyle is widely evidenced, little is known about these exposures in the periconception period. We investigated associations between periconceptional lifestyle behaviour change and adverse pregnancy outcomes.
Methods
A retrospective secondary analysis took place within a prospective multicentre cohort study in the Netherlands, including 3,684 pregnant women. Baseline characteristics and preconceptional and first-trimester lifestyle behaviours were assessed through a self-administered questionnaire. Adverse pregnancy outcomes (hypertensive disorders in pregnancy (HDP), small for gestational age (SGA), gestational diabetes (GDM) and spontaneous preterm birth (sPTB)) were reported by healthcare professionals. Data were collected between December 2012 - January 2014 and analysed between August - December 2019, using multivariate logistic regression.
Results
Overweight, and especially obese women, have the highest odds of developing any adverse pregnancy outcome (adjusted odds ratio (aOR) 1.61 (95%CI 1.31–1.99) and aOR 2.85 (95%CI 2.20–3.68), respectively), particularly HDP and GDM. Women who prenatally quit smoking had a lower odds of SGA (aOR 1.14 (95%CI 0.59–2.21)), while women who continued smoking attained higher odds (aOR 1.91 (95%CI 1.05–1.15)). Women who did not use folic acid tended to have a higher odds of developing adverse pregnancy outcomes (aOR 1.28 (95%CI 0.97–1.69)), while women who prenatally started folic acid did not (aOR 1.01 (95%CI 0.82–1.25)).
Conclusions
Our results indicate that smoking cessation, having a lower BMI and initiating folic acid supplements preconceptionally may decrease the risk of adverse pregnancy outcomes. Therefore, intervening as early as the preconception period could benefit the health of future generations.
Effects of Chinese Medical Pricing Reform on the Structure of Hospital Revenue and Healthcare Expenditure in County Hospital: An Interrupted Time Series Analysis
Authors: Liu Mengling , Jia Mingyuan , Lin Qian , Zhu Jiawei , Wang Dong 
Published: February 2021

Abstract
Background

China has initiated a medical pricing reform to combat the overuse of drugs and relieve the financial burden of patients. This paper aims to analyze the effect of medical pricing reform on revenue structure and healthcare expenditure of county public hospitals in Guangdong province.
Methods

Based on the monthly data from January 2013 to August 2019, we use interrupted time series design to evaluate the effects of medical pricing reform on healthcare expenditure in both outpatients and inpatients. A counterfactual is also established to examine the net effect of the policy.
Results

The proportion of drug revenue decreased from 35% in 2015 to 29.7% in 2019, and the revenue from medical services and inspection increased 3.2% and 3% respectively. Meanwhile, the increasing trend of total expenditure and its main components is slowed down, especially the drug expense and medical consumable expense for inpatients after the Zero Mark-up Drug policy. However, the growth of inspection expense for outpatients continues to increase, while the healthcare expenditure for inpatients experiences an instant increase after the Zero Mark-up Medical Consumables policy.
Conclusion

The medical pricing reform is a valuable attempt in controlling the unreasonable increase of medical expenses. In the meantime, the unexpected increase in inspection expenditure and insufficient compensation from medical service adjustment should draw the attention of the policymakers.
Quantitative Visual Tests in Primary Open-Angle Glaucoma Patients According to Three Different Lights With Different Color-Rendering Index
Authors: Kim Sang Woo , Go YoungWook , Kang Sangok , Lee Chang Kyu 
Published: February 2021

Abstract
Purpose

To compare quantitative visual tests, such as visual acuity, contrast sensitivity, and color vision tests in patients with primary open-angle glaucoma (POAG) patients according to three different light systems with different color-rendering index (CRI).
Methods

This was a cross-sectional study of 20 eyes in 20 patients with POAG. Three different light systems consisting of a 3-band fluorescent lamp (CRI 80), a white LED (CRI 75), and a quantum dot LED (CRI > 95) were used. All lights had the same illuminance of 230 lux to exclude illuminance effects. The visual testing included best-corrected visual acuity (BCVA) using an ETDRS chart, a CSV-1000E contrast test, and a color test performed by the Farnsworth Munsell 100-hue test.
Results

There was no significant difference in BCVA (p = 0.86). There were no significant differences in the detail contrast tests according to the three light systems (p = 0.98, p = 0.93, p = 0.86, respectively, p = 0.70). There was no significant difference between the three light systems (p = 0.08). However, the color test scores with a quantum dot LED were significantly lower than those of the white LED and 3-band fluorescent lamp (p = 0.03 and 0.047, respectively).
Conclusion

POAG patients did not show significant differences in visual acuity scores and contrast test scores, expressed as black and white symbols, according to the different light systems. However, POAG patients tested under a quantum dot LED (CRI > 95) could distinguish color differences better than in the other light systems.
Liquid-phase Exfoliated Two-dimensional Bi Nanosheet as a Durable Electrocatalyst for Hydrogen Evolution Reaction
Authors: Duan Chenguang , Liu Huating , Huang Zongyu , Qiao Hui , Zhou Yang , Liao Gengcheng , Liu Yundan , Qi Xiang 
Published: February 2021

Abstract
Electrocatalytic hydrogen evolution is an exercisable way to achieve large-scale application of hydrogen energy. It is of great significance to develop an effect, stable and cost-effective electrocatalyst. Here, we applied the two-dimensional (2D) bismuth (Bi) to the electrocatalytic hydrogen evolution, and proposed the strategies to enhance the catalytic performance of the catalyst. The exfoliated Bi nanosheets via sonication assisted liquid-phase exfoliation display higher electrocatalytic activity (overpotential of -958 mV vs RHE at 10 mA cm− 2) compared to the bulk counterpart. Theoretical calculations about Gibbs free energy from the hydrogen adsorption for 1 layer, 2 layers and 5 layers Bi also manifest the decrease of thickness is favorable for hydrogen evolution reaction (HER). To further evaluate the electrocatalytic performance of Bi nanosheets, the typical parameters measured in different H+ concentration (C[H+]) are carried out. The improved catalytic activity obtained in 0.5 M H2SO4 is attributed to enhancing the hydrogen adsorption and accelerating the charge transport on the surface of catalyst. Moreover, the durability of Bi nanosheets electrode has been texted, where the current is not evident fluctuation during the 40000 s electrolysis measurement indicating its excellent stability. The present work expands the application of Bi in the catalysis and provides the simple strategies to improve its hydrogen evolution performance.
Analysis of The Mixed Effects Regression Model for Clustered Count Response Data
Authors: Aguade Aragaw Eshetie , Begari B.Muniswamy 
Published: February 2021

Abstract
BackgroundThe Poisson regression model is useful for analyse count data, but, when the observations are correlated the Poisson estimate will be biased. Whereas, when the over-dispersion and heterogeneity problems occur the imposition of the Poisson model underestimate the standard error and overestimate the significance of the regression parameters. Therefore, the objective of this paper was to develop a test statistic to model and predict clustered count response data via the application and simulation data.MethodsThis paper concentrated on the clustered count data model to take into account heterogeneity. Accordingly, we developed a score test based on the multilevel Poisson model for testing heterogeneity with the alternative Poisson regression model. In addition, for the model application, we used the EDHS children`s data. Therefore, to evaluate the proposed model, we used both simulation and application data.ResultsSimulation results showed that the proposed score test has high power to predict and used to control heterogeneity between groups. Oromia, Amhara, and SNNPR are among the regions with the highest child mortality rates (Table 1). The results indicated that women who made marriage a mean age of 16 years and gave birth to the first child a mean age of 18 years and 8 months. Table 1 showed that 81% of all child deaths have recorded in rural areas. 78% of child families were illiterate, as a result, 75% of children don't have access to latrines and drinking water. Rivers and open-source waters are the common sources of drinking water, which comprised 79% of the total water supply. Therefore, from the research finding, it is possible to conclude that most child mortality is due to scarcity of water.ConclusionThe Power of test estimates indicated that the proposed method was better than the existing models. All covariant and dummy explanatory variables have a significant effect on the deaths of children. Hence, the multilevel Poisson model results indicated that there exists high variability among regions for the deaths of children. Therefore, this work suggested that the applications of the random-effects model provided a simple and robust means to predict the count response data model.
Sarcoma Molecular Pathological Diagnosis of FFPE Tissues without Dewaxing based on Lipidomic Profiles using SpiderMass Technology
Authors: Ogrinc Nina , Caux Pierre-Damien , Robin Yves-marie , Bouchaert Emmanuel , Fatou Benoit , Ziskind Mickael , Focsa Cristian , Bertin Delphine , Tierny Dominique , Takats Zoltan , Salzet Michel , Fournier Isabelle 
Published: February 2021

Abstract
Background :Formalin-fixation and paraffin-embedding (FFPE) is the worldwide gold standard for tissue preservation and routine pathology for general and cancer diagnostics. Despite robust histopathology methods, accurate diagnostic remains difficult for certain cases. Overall, the entire process is time-consuming, labor-intensive and doesn’t reach the right sensitivity and specificity. Lipids are central players in cancer, but they are not routinely used in diagnostics because of the sample processing which limits their detection, and lipid analysis directly from unprocessed FFPE tissues has never been reported. Methods :We present a new method for cancer diagnostics based on tissue-lipidomic signatures through rapid screening of FFPE without dewaxing using Water-Assisted Laser Desorption Ionization Mass Spectrometry (WALDI-MS) and deep-learning. Results :We show on a cohort of canine and human sarcoma tissues that classification for sarcoma sub-typing is possible and enables diagnosing blind samples in less than five minutes, with over 95% probability to discriminate specific subtypes. Conclusion: We have revealed the possibility to create a rapid diagnostic platform to screen clinical FFPE tissues with minimal sample preparation for molecular pathology.
Comparative Pathology of Experimental Avibacterium Paragallinarum Infection in Chicken and Japanese Quail
Authors: Balouria Aadish , Deshmukh Sidhartha , Banga Harmanjit Singh , Chawak Milind Madhukar , Dash Shakti Kant , Thakur Aneesh 
Published: February 2021

Abstract
IntroductionAvibacterium paragallinarum (Av.paragallinarum) is the causative agent of infectious coryza (IC) in chicken, an economically devastating disease of poultry industry. Despite the availability of effective vaccines against IC, the infection is rampant in unorganized poultry sector. Moreover, reports of wider host susceptibility in poultry farms and under field conditions are also emerging. Av.paragallinarum affects the upper respiratory tract of birds and has a predilection for the nasal turbinates. However, no study has systematically evaluated the early pathological changes and mucosal immune responses during Av. paragallinarum infection in the nasal turbinates of chicken. Furthermore, the use of Japanese quail as an alternate experimental animal model than chicken in IC remain unexplored. Here, we examined clinical signs, gross and histopathological changes at the nasal turbinates following experimental Av. paragallinarum infection in chicken and Japanese quail. MethodsWe developed a comprehensive scoring system for recording gross and histopathologic lesions during infection. ResultsOur data show that chicken have a higher susceptibility to Av.paragallinarum infection than the Japanese quail. Japanese quail had significantly lower gross as well as histopathology score in nasal turbinates as compared to infected chicken. Importantly, chicken at an early age i.e. 2.5 weeks were able to induce mucosal immune responses against Av. paragallinarum infection. ConclusionOur findings suggest that there are demonstrable differences in the disease pathology and host immune response to Av. paragallinarum infection in chicken and Japanese quail and warrant further investigation for the use of Japanese quail as an animal model for IC.
Piperazine-Substituted Chalcones: A New Class of MAO-B, AChE, and BACE-1 Inhibitors for the Treatment of Neurologic Al Disorders
Authors: Mathew Bijo , Oh Jong Min Min , Al-Rashed Sarah , Batiha Gaber El-Saber , Parambi Della Grace Thomas , Gambacorta Nicola , Nicolotti Orazio , Kim Hoon 
Published: February 2021

Abstract
Eleven piperazine-containing 1,3-diphenylprop-2-en-1-one derivatives (PC1-PC11) were evaluated for their inhibitory activities against monoamine oxidases (MAOs), cholinesterases (ChEs), and β-site amyloid precursor protein cleaving enzyme 1 (BACE-1) with a view toward developing new treatments for neurological disorders. Compounds PC10 and PC11 remarkably inhibited MAO-B inhibition with IC50 values of 0.65 and 0.71 µM, respectively. Ten of the eleven compounds weakly inhibited AChE and BChE with > 50% of residual activities at 10 µM, although PC4 inhibited AChE by 56.6% (IC50 = 8.77 µM). Compound PC3 effectively inhibited BACE-1 (IC50 = 6.72 µM), and PC10 and PC11 moderately inhibited BACE-1 (IC50 =14.9 and 15.3 µM, respectively). Reversibility and kinetic studies showed that PC10 and PC11 were reversible and competitive inhibitors of MAO-B with Ki values of 0.63 ± 0.13 and 0.53 ± 0.068 µM, respectively. ADME predictions for lead compounds revealed that PC10 and PC11 have central nervous system (CNS) drug-likeness. Molecular docking simulations showed that fluorine atom and trifluoromethyl group on PC10 and PC11, respectively, interacted with the substrate cavity of the MAO-B active site. Our results suggested that PC10 and PC11 be considered potential candidates for the treatment of neurological disorders such as Alzheimer’s disease and Parkinson’s disease.
Impact of Educational Self-Efficacy Enhancement Program on Quality of Life, Pain Management, Self-Efficiency Behaviors and Its Impact of Satisfaction among Adults Diabetic Patients with Peripheral Neuropathy Pain: A Randomized Control Trial
Authors: Abu-Shennar Jawad AHMAD , Bayraktar Nurhan 
Published: February 2021

Abstract
Background: Peripheral neuropathy pain is common among adult diabetic patients worldwide. Lifestyle behavior modification such as proper diet and exercise, quitting smoking, weight control and regular follow up, as well as enhancement of self-efficacy among adult diabetic patients can be helpful in managing peripheral neuropathy pain and, therefore, improving their quality of life and satisfaction.Aims: This study aims to investigate educational self-efficacy enhancement program's impact on pain management, self-efficiency behaviors, quality of life and satisfaction among adult diabetic patients with peripheral neuropathy pain.Materials and Methods: This randomized controlled study was conducted between October and March 2020 at the Jordanian Ministry of Health settings, where 72 adult diabetic patients with peripheral neuropathy pain were randomly assigned into research group (n = 36) or control group (n = 36), after obtaining ethical approval and informed consents. An educational self-efficacy enhancement program was implemented in the research group only. The instrument used for data collection consisted of Demographic Data Form, Diabetes Pain Intensity Scale, Self-Efficacy Scale, Quality of Life Questionnaire (EQ-5D) and Patient Satisfaction Questionnaire.Results: Statistically significant improvements in pain levels (p = 0.020), self-efficacy score (p = 0.000), quality of life scores (p = 0.000) and best imagined health status (p = 0 .000) were found among research group participants. Furthermore, multiple-linear regression models showed that the implementation of educational self-efficacy enhancement program significantly increased the explained variance of self-efficacy scores (R2 = 0.746, p = 0.000) and quality of life scores (R2 = 0.746, p = 0.000). At the end of implementation, research group participants showed a high overall satisfaction levels (4.33 out of 5). Conclusion: The findings suggest that an educational self-efficacy enhancement program is effective in modifying adult diabetic patients’ lifestyle, enhancing self-efficacy behaviors, managing peripheral neuropathy pain, improving quality of life and satisfaction among adult diabetic patients. It is, therefore, recommended to expand the implementation of this program among adult diabetic patients with peripheral neuropathy pain.
Development of a Combination Fermentation Strategy to Simultaneously Increase Biomass and Enzyme Activity of D-amino acid Oxidase&nbsp;Expressed in Escherichia Coli
Authors: Xu Jian-Miao , Cao Hui-Ting , Wang Ming , Ma Bao-Jian , Wang Liu-Yu , Zhang Kai , Cheng Feng , Xue Yaping , Zheng Yu-Guo 
Published: February 2021

Abstract
d-amino acid oxidase (DAAO) is widely used in the industrial preparation of l-amino acids, and cultivating Escherichia coli (E. coli) expressing DAAO for the biosynthesis of l-phosphinothricin (l-PPT) is very attractive. At present, the biomass production of DAAO by fermentation is still limited in large-scale industrial applications because the expression of DAAO during the fermentation process inhibits the growth of host cells, which limits higher cell density. In this study, the factors that inhibit the growth of bacterial cells during a 5 L fed-batch fermentation process were explored, and the fermentation process was optimized by co-expressing catalase (CAT), by balancing the biomass and the enzyme activity, and by adding exogenous d-alanine (d-Ala) to relieve the limitation of DAAO on the cells and optimize fermentation. Under optimal conditions, the DO-STAT feeding mode with DO controlled at 30% ± 5% and the addition of 27.5 g/L lactose mixed with 2 g/L d-Ala during induction at 28 °C resulted in the production of 26.03 g dry cell weight (DCW)/L biomass and 390.0 U/g DCW specific activity of DAAO; an increase of 78% and 84%, respectively, compared with the initial fermentation conditions. The fermentation strategy was successfully scale-up to a 5000L fermenter.
Sexual function in premenopausal women with breast cancer
Authors: Pontes Filipa , Magalhaes Joana , Basto Raquel , Felix Rita , Mariano Monica , Sousa Gabriela 
Published: February 2021

Abstract
Background: This cross-sectional study assessed Female Sexual Function Index (FSFI) scores in premenopausal women with breast cancer diagnosis. We aimed to determine variables that are associated with sexual dysfunction and if these thematic is being addressed by healthcare professionals.Methods: The FSFI questionnaire was administered to 199 premenopausal women 6 months after completing breast cancer initial treatment (surgery, radiation therapy or chemotherapy). A demographic questionnaire was administered. Scores were compared between sexually active women sub-groups. Questions, regarding FSFI acceptability and to find out if healthcare professionals are addressing these thematic were included.Results: The study included 199 women. Thirty-three were excluded from analysis because they declared no sexual activity in the 4 weeks before the survey. Ninety-seven women met the FSFI cutoff score for a sexual dysfunction. FSFI Scores were significantly lower in women treated with radiation therapy and in women treated with radical mastectomy and lumpectomy when compared with mastectomy with immediate reconstruction. One hundred and thirty-eight women were never or rarely questioned about their sexual health even though 71.2% reported feeling comfortable about sharing this problematic with doctors. Both sexually active and non-active women provided positive feedback about the FSFI.Conclusion: FSFI scores were compatible with sexual dysfunction in more than half of the sexually active women. Women treated with radiation therapy, radical mastectomy and lumpectomy had significant lower FSFI scores. With desirable acceptability, the FSFI is suitable for screening for sexual dysfunction in premenopausal women with
Dissociated Response and Clinical Impact in Patients Treated With Nivolumab Monotherapy
Authors: Sato Yuki , Morimoto Takeshi , Hara Shigeo , Nagata Kazuma , Hosoya Kazutaka , Nakagawa Atsushi , Tachikawa Ryo , Tomii Keisuke 
Published: February 2021

Abstract
ObjectivesImmune checkpoint inhibitors (ICIs) are effective for previously treated patients with advanced non-small cell lung cancer (NSCLC). However, an unconventional response pattern is sometimes encountered. A dissociated response (DR), characterized by some lesions shrinking and others growing, has been recognized with ICI treatment. In this study, we examined the characteristics and treatment outcomes of DR in previously treated NSCLC patients, receiving nivolumab monotherapy.Patients and MethodsWe conducted a retrospective cohort study of previously treated patients with advanced NSCLC who received nivolumab. We assessed the tumor response of each organ using the Response Evaluation Criteria in Solid Tumors (RECIST) criteria at the first radiologic evaluation. We investigated treatment outcome and compared overall survival using the Kaplan-Meier Method and log-rank tests. Further, we conducted the same analysis in patients who had previously received chemotherapy or tyrosine kinase inhibitor therapy in our hospital.ResultsBetween April 2016 and September 2018, 107 patients who received nivolumab fulfilled the inclusion criteria. Of them, 5 (5%) patients showed a DR. There were no specific differences in characteristics between DR and non-DR cases. Patients showing DR had significantly longer overall survival than those showing concordant progressive disease (46.9 vs. 8.2 months, p = 0.038). The frequencies of DR in the ICI, chemotherapy, and tyrosine kinase inhibitor-treated cohorts were 5%, 1%, and 4%, respectively.ConclusionDR was uncommon, but this presented a distinctive pattern of nivolumab response. Some patients might benefit from continuing nivolumab therapy and may achieve a longer overall survival.