bluuebunny/crossref_metadata_2025_split huggingface dataset:
Traditional Chinese Medicine Syndromes Classification Associates with Tumor Cell and Microenvironment Heterogeneity in Colorectal Cancer: a Single Cell RNA Sequencing Analysis
Authors: Lu Yiyu , Zhou Chungen , Zhu Meidong , Fu Zhiliang , Shi Yong , Li Min , Wang Wenhai , Zhu Shibo , Jiang Bin , Luo Yunquan , Shibing Su 
Published: October 2021

Abstract
Background: Colorectal cancer (CRC) is one of the common gastrointestinal malignancies, tumor heterogeneity is the main cause of refractory CRC. Syndrome differentiation is the premise of individualized treatment of traditional Chinese medicine (TCM), but TCM syndrome lacks objective identification in CRC. Methods: In this study, we using scRNA-seq technology, investigate the significance of tumor heterogeneity in TCM syndromes classification on CRC. Results: The results showed that 662 cells isolated from 11 primary CRC tumors are divided into 14 different cell clusters, and each cell subtype and its genes have different functions and signal transduction pathways, indicating significant heterogeneity. CRC tumor cell clusters have different proportions in Excess, Deficiency and Deficiency-Excess syndromes, and have their own characteristic genes, gene co-expression networks, gene functional interpretations as well as monocle functional evolution. Moreover, there were significant differences between the high expressions of MUC2, REG4, COL1A2, POSTN, SDPR, GPX1, ELF3, KRT8, KRT18, KRT19, FN1, SERPINE1, TCF4 and ZEB1 genes and Excess and Deficiency syndrome classification in CRC (P<0.01). Conclusions: The study suggested that the Excess and Deficiency syndromes classification of CRC may be related to tumor heterogeneity and its microenvironment.
Isorhamnetin Protects Zearalenone-induced Damage via the PI3K/Akt Signaling Pathway in Porcine Granulosa Cells
Authors: Li Xiaoya , Chen Huali , Zhang Zelin , Li Xiaodi , Duan Jiaxin , Hua Rongmao , Yang Li , Cheng Jianyong , Li Qingwang 
Published: October 2021

Abstract
Background: Zearalenone (ZEA) is widely derived from moldy cereal grain, which has adverse effects on animal reproduction. In particular, pigs are more sensitive to ZEA-induced toxicity than other animals. Isorhamnetin, a flavonoid has extensive of pharmacological activities. However, little is known about the effects of isorhamnetin on reproduction. Thus, it would be interesting to clarify the effect and the underlying molecular mechanism of isorhamnetin involvement in ZEA-induced cytotoxicity in porcine granulosa cells.Results: Our findings showed that isorhamnetin suppressed ZEA-induced apoptosis by regulating Bcl-2 and Bax protein changes. Changes in intracellular Ca2+ levels and CHOP, ATF6, GRP78 indicated that isorhamnetin rescued ZEA-induced endoplasmic reticulum stress (ERS). Furthermore, isorhamnetin prevented ZEA-induced reactive oxygen species (ROS) via P38 signaling pathway. Mechanistically, isorhamnetin stimulated the expression of PCNA and CyclinD, thereby raising the ratio of S phases cells in response to ZEA-induced apoptosis via PI3K/Akt signaling pathway. Isorhamnetin also recovered ZEA-induced steroidogenesis disorder by regulating steroidogenic enzyme gene and proteins (FSH-R, CYP19A1). Conclusions: Collectively, these findings show that isorhamnetin protects granulosa cells from ZEA-induced damage via the PI3K/Akt signaling pathway, which promotes proliferation, alleviates steroidogenesis disorder, ERS and oxidative stress.
The pattern of alternative splicing in lung adenocarcinoma shows novel events correlated with tumorigenesis and immune microenvironment
Authors: Qiu Wensheng 
Published: October 2021

Abstract
Lung adenocarcinoma (LUAD) is the leading cause of cancer deaths worldwide due to the lack of early diagnostic markers and specific drugs. Previous studies have shown the association of LUAD growth with aberrant alternative splicing (AS). Herein, clinical data of 535 tumor tissues and 59 normal tissues were extracted from the TCGA database. Each sample was analyzed using the ESTIMATE algorithm; a comparison between higher and lower score groups (stromal or immune) was made to determine the overall- and progression-free survival-related differentially expressed AS (DEAS) events. We then performed unsupervised clustering of these DEASs, followed by determining their relationship with survival rate, immune cells, and the tumor microenvironment (TME). Next, two prognostic signatures were developed using bioinformatics tools to explore the prognosis of cases with LUAD. Five OS- and six PFS-associated DEAS events were implemented to establish a prognostic risk score model. When compared to the high-risk group (HRG), the PFS and OS of the low-risk group (LRG) were found to be considerable. Additionally, a better prognosis was found considerably associated with the ESTIMATE score of the patients as well as immune cells infiltration. Our analysis of AS events in LUAD not only helps to clarify the tumorigenesis mechanism of AS, but also provides ideas for revealing potential prognostic biomarkers and therapeutic targets.
Spatial Pattern And Determinants of Institutional Delivery in Ethiopia: Spatial and Multilevel Analysis Using 2019 Ethiopian Mini Demographic and Health Survey.
Authors: Awol Mukemil , Dirirsa Dejene Edosa , Jemal Kemal 
Published: October 2021

Abstract
Background: In Ethiopia, despite the progress that has been made to improve maternal and child health, the proportion of births occurring at health institutions is still very low (26%), Which significantly contribute to a large number of maternal death 412 deaths/100,000 live births. Therefore, this study intended to determine spatial pattern and factors affecting institutional delivery among women who had live birth in Ethiopia within five years preceding survey.Method: Data from 2019 mini Ethiopian demographic and health survey were used. Taking into account the nested structure of the data, multilevel logistic regression analysis has been employed to a nationally representative sample of 5753 women nested with in 305 communities/clusters.Result: A significant heterogeneity was observed between clusters for institutional delivery which explains about 57% of the total variation. Individual-level variables: primary education (OR= 1.8: 95% CI: 1.44-2.26), secondary education (OR= 3.65: 95% CI: 2.19- 6.1), diploma and higher (OR= 2.74: 95% CI: 1.02 – 7.34), women who had both Radio and Television were 4.6 times (OR= 4.6; 95% CI: 2.52, 8.45), four and above Antenatal visit (AOR=2.72 ,95% CI:2.2, 3.34), rich wealth index (OR= 2.22; 95% CI: 1.62- 2.99), birth interval for 18 to 33 months (OR= 1.8; 95% CI: 1.19, 2.92), and women who space birth for 33 and above months (OR= 2.02; 95% CI: 1.3, 3.12) were associated with institutional delivery. Community level variables, community high proportion of antenatal visit (OR= 4.68; 95% CI: 4.13- 5.30), and Region were associated with institutional deliveryConclusion: A clustered pattern of areas with low institutional delivery was observed in Ethiopia. Both individual and community level factors found significantly associated with institutional delivery theses showed the need for community women education through health extension programs and community health workers. And the effort to promote institutional delivery should pay special attention to antenatal care, less educated women and interventions considering awareness, access, and availability of the services are vital for regions.
Phenotypic and transcriptomic responses of cultivated sunflower seedlings (Helianthus annuus L.) to four abiotic stresses
Authors: Barnhart Max H. , Masalia Rishi R. , Mosley Liana J. , Burke John M. 
Published: October 2021

Abstract
Plants encounter and respond to numerous abiotic stresses during their lifetimes. These stresses are often related and could therefore elicit related responses. There are, however, relatively few detailed comparisons between multiple different stresses at the molecular level. Here, we investigated the phenotypic and transcriptomic response of cultivated sunflower (Helianthus annuus L.) seedlings to three water-related stresses (i.e., dry-down, an osmotic challenge with polyethylene glycol 6000 [PEG], and salt stress), as well as a generalized low-nutrient stress. Our goal was to identify commonalities in the response to the three water-related stresses and compare them to a distinct low-nutrient stress. All four stresses negatively impacted seedling growth, with the low-nutrient stress having a more divergent response from control as compared to the water-related stresses. Observed phenotypic responses were consistent with expectation for growth in low-resource environments, including increased (i.e., less negative) carbon fractionation values and leaf C:N ratios, as well as increased belowground biomass allocation. Analysis of the leaf and root transcriptome under each stress scenario revealed that most genes were differentially expressed in response to multiple stresses. The number of differentially expressed genes (DEGs) under stress was greater in leaf tissue, but roots exhibited a higher proportion of DEGs unique to individual stresses. Overall, the three water-related stresses had a more similar transcriptomic response to each other vs. low-nutrient stress, though this pattern was more pronounced in root tissue than in leaf tissue. In contrast with the results of our differential expression analysis, co-expression network analysis revealed that the response to each of the four stresses in our study were generally non-overlapping and there was little indication of a shared co-expression response despite the majority of DEGs being shared between multiple stresses. Importantly, PEG stress, which is often used to simulate drought stress in experimental settings, had little transcriptomic resemblance to true water limitation (i.e., dry-down) in our study calling into question its utility as a means for simulating drought.
Exploring Variation of Coverage and Access to Dental Care for Adults in 11 European Countries: A Vignette Approach
Authors: Winkelmann Juliane , Rossi Jesús Gómez , Schwendicke Falk , Dimova Antonia , Atanasova Elka , Habicht Triin , Kasekamp Kaija , Gandré Coralie , Or Zeynep , McAuliffe Una , Murauskiene Liubove , Kroneman Madelon , Jong Judith , Kowalska-Bobko Iwona , Badora-Musiał Katarzyna , Motyl Sylwia , Augusto Gonçalo Figueiredo , Pažitný Peter , Kandilakil Daniela , Löffler Lubica , Lundgren Carl , Janlöv Nils , Ginneken Ewout van , Panteli Dimitra 
Published: October 2021

Abstract
Background: Oral health has received increased attention over the past few years coupled with rising awareness on the impact of limited dental care coverage for oral health and general health and well-being. The purpose of the study was to compare the statutory coverage and access to dental care for adult services in 11 European countries using a vignette approach.Methods: We used three patient vignettes to highlight the differences of the dimensions of coverage and access to dental care (coverage, cost-sharing and accessibility). The three vignettes describe typical care pathways for patients with the most common oral health conditions (caries, periodontal disease, edentulism). The vignettes were completed by health services researchers knowledgeable on dental care, dentists, or teams consisting of a health systems expert working together with dental specialists.Results: Completed vignettes were received from 11 countries, including Bulgaria, Estonia, France, Germany, Republic of Ireland (Ireland), Lithuania, the Netherlands, Poland, Portugal, Slovakia and Sweden. While emergency dental care, tooth extraction and restorative care for acute pain due to carious lesions are covered in most responding countries, root canal treatment, periodontal care and prosthetic restoration often require cost-sharing or are entirely excluded from the benefit basket. Regular dental visits are also limited to one visit per year in many countries. Beyond financial barriers due to out-of-pocket payments, patients may experience very different kinds of physical barriers to access dental care. Major access barriers to public dental care represent the limited availability of contracted dentists especially in rural areas and the unequal distribution and lack of specialised dentists.Conclusions: According to the results, statutory coverage of dental care varies across European countries while access barriers are largely similar. Many dental services require substantial cost-sharing in most countries which in turn leads to high out-of-pocket spending. The individual socioeconomic status is thus a main determinant for access to dental care, but also other factors such as geography, age and comorbidities can inhibit access and affect outcomes. Moreover, coverage in most oral health systems is targeted at treatment and less at preventative oral health care.
Transitions of zonal flows in a two-layer quasi-geostrophic ocean model
Authors: Şengül Mustafa Taylan , Chekroun Mickael , Dijkstra Henk , Wang Shouhong 
Published: October 2021

Abstract
We consider a 2-layer quasi-geostrophic ocean model where the upper layer is forced by a steady Kolmogorov wind stress in a periodic channel domain, which allows to mathematically study the nonlinear development of the resulting flow. The model supports a steady parallel shear flow as a response to the wind stress. As the maximal velocity of the shear flow (equivalently the maximal amplitude of the wind forcing) exceeds a critical threshold, the zonal jet destabilizes due to baroclinic instability and we numerically demonstrate that a first transition occurs. We obtain reduced equations of the system using the formalism of dynamic transition theory and establish two scenarios which completely describe this first transition. The generic scenario is that two modes become critical and a Hopf bifurcation occurs as a result. Under an appropriate set of parameters describing midlatitude oceanic flows, we show that this first transition is continuous: a supercritical Hopf bifurcation occurs and a stable time periodic solution bifurcates. We also investigate the case of double Hopf bifurcations which occur when four modes of the linear stability problem simultaneously destabilize the zonal jet. In this case we prove that, in the relevant parameter regime, the flow exhibits a continuous transition accompanied by a bifurcated attractor homeomorphic to $S^3$. The topological structure of this attractor is analyzed in detail and is shown to depend on the system parameters. In particular, this attractor contains (stable or unstable) time-periodic solutions and a quasi-periodic solution.
18 F-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography imaging for the diagnosis of immune checkpoint inhibitors associated myocarditis
Authors: Ederhy Stéphane , Devos Perrine , Pinna Bruno , Funck-Brentano Elisa , Abbar Baptiste , Fenioux Charlotte , Cohen Ariel A. , Moslehi Javid , Bretagne Marie , Allenbach Yves , Kharroubi Dris , Salem Joe-Elie 
Published: October 2021

Abstract
Immune-checkpoint inhibitors (ICI) have profoundly improved the prognosis of cancer patients but are associated with life-threatening myocarditis (incidence≤1%).The diagnosis of ICI-myocarditis remains challenging necessitating the need for novel diagnostic strategies.This single center cohort included 61 consecutive patients referred to our cardio-oncology unit for a suspicion of ICI-myocarditis with a positive troponin, between March 2019 and March 2021. In the 31 patients with suspected ICI-myocarditis with available FDG-PET, the median delay between admission and the first available FDG-PET performed was 12 days [interquartile-range:9-30]. Patients received ICI (ICI-monotherapy: 24/31, 77% and ICI-combination therapy: 7/31, 23%), mainly for lung cancer (n=10), melanoma (n=5), and kidney cancer (n=3). FDG-PET was performed using a standardized protocol involving dietary measures prior to PET, including fasting of at least 6h and a fat enriched diet without carbohydrates for 24h. FDG-PET platforms included Biograph-mCT-Flow Siemens (n=9/34, 26%) or Discovery-MI-5-Ring General Electric (n=25/34, 74%) devices and analysed using Singo.via Workstation (Siemens) by a nuclear medicine physician blinded to patients’ medical records. Interpretation of FDG-PET was based on the following classification: 1/No FDG uptake, 2/Diffuse FDG uptake, 3/Focal FDG uptake, 4/Focal on diffuse FDG uptake.An abnormal cardiac fixation on FDG-PET suggestive of myocarditis was observed in only 2/21 (9.5%) patients with otherwise definite ICI-myocarditis (1 diffuse, 1 focal), not different in proportion versus 1/7 (14.3%, 1 focal) patient without ICI-myocarditis (p>0.99). The sensitivity, specificity, positive predictive and negative predictive values (with their 95% confidence-interval) of FDG-PET for ICI-myocarditis was 9.5% (1.2-30.4%), 85.7% (42.1-99.6%), 66% (17.5-95%), 24% (18.5-30.6%), respectively. Only 2/14(14.2%) FDG-PET were positive despite being performed at a time in which ICI-myocarditis was fully active with troponin levels over ten-times the normal values versus 0/6(0%,p>0.99) for FDG-PET performed when troponin levels were abnormal but below ten-times the upper limit. Similarly, there was no difference in FDG-PET positivity rate for exams performed within 14 days (1/7, 14.3%; plus 3 inconclusive exams) versus those performed after 14 days (1/14, 7.2%; no inconclusive exams; p>0.99) of hospital admission.Altogether, our study suggests that FDG-PET has a limited diagnostic value for the diagnosis of ICI-Myocarditis.
ARMONIZAR ALMA Y CUERPO A TRAVÉS DE LA BELLEZA. LA APORTACIÓN DEL IDEALISMO
Authors: Bosch Magdalena 
Published: October 2021

Uno de los grandes desafíos de la actividad educativa es aprender a armonizar alma y cuerpo; es un reto personal —de cada sujeto singular—, pero en el que se necesitan recursos que la pedagogía puede estudiar y enseñar.
La unión de alma y cuerpo es también uno de los más antiguos problemas de la filosofía, tanto en una aproximación teórica como por sus consecuencias prácticas. (Además de las reflexiones metafísicas que pueden realizarse en torno a este tema).
Elpropósito de este escrito es redescubrir algunas sugerencias del idea-lismo acerca de un equilibrio posible entre alma y cuerpo. Me referiré aquí a una parte muy concreta del idealismo: aquella que ofrece una visión positiva del hombre, y que se caracteriza por su optimismo, más que por la abstracción. Se trata del momento más moderado del Romanticismo, que surge en sus inicios.
DESARROLLAR LA COMPETENCIA REFLEXIVA EN LA EDUCACIÓN SUPERIOR. DIEZ PROPUESTAS PARA EL AULA UNIVERSITARIA
Authors: Domingo Roget Ángeles 
Published: October 2021

Las conclusiones de esta investigación realizada a lo largo del curso 2008 en las aulas universitarias de la Facultad de Educación de la Universidad Internacional de Cataluña, se sitúan en un marco paradigmático crítico-reflexivo —superado el conocimiento por reproducción o transmisión— y apuestan con determinación por la formación reflexiva de los futuros docentes. Las propuestas de desarrollo de la competencia reflexiva en el aula universitaria son adecuadas para todas las titulaciones, aunque mi experiencia se circunscribe a titulaciones de formación docente. Las diez propuestas que se presentan propician, en cada estudiante, la construcción de su proyecto personal y promueven la competencia reflexiva y activa en los escenarios profesionales, reforzándose de este modo la profesionalización docente. Fomentar la competencia reflexiva durante la formación inicial universitaria ha producido resultados de profesionalización en los docentes egresados de la Universidad, mismos que podemos sintetizar así:

Mayor capacidad de resolver situaciones prácticas como profesionales expertos.
Articulación más profunda en su quehacer docente de teoría y práctica, conocimiento formal y práctico, criterio científico y compromiso ético y social.
Mayor preparación metodológica para innovar e investigar a partir de su trabajo docente.
ENTRETEJER EL PENSAR PARA RELACIONAR EL HACER
Authors: Zubillaga Itziar 
Published: October 2021

Este texto se dirige a los protagonistas de la educación superior. Su principal intención es expresar cómo, desde el aula universitaria, puede lograrse que el estudiante —con apoyo del docente y con base en el contenido curricular derivado en actividades concretas—, elabore productos de aprendizaje1, acordes al desarrollo del pensamiento complejo.
El artículo hace hincapie en la educación superios áulica, intentando mejorar el proceso de enseñanza-aprendizaje; mejorarlo desde la perspectiva de complejizar el pensar para expresarlo en un hacer con sentido de aprendizaje profundo; hacer camino para lograr que el docente y el estudiante «utilicen los procesos de nivel cognitivo superior» (Biggs, 2005), considerando a los productos de aprendizaje como medios para ese logro.
FORMACIÓN CIUDADANA Y AUTORREGULACIÓN: CONCEPTOS CLAVES PARA LA PRÁCTICA EDUCATIVA
Authors: Beuchat Reichardt Marianne 
Published: October 2021

Se expone la relación de los conceptos de autorregulación del aprendizaje y formación ciudadana en el marco imperante de formar ciudadanos socialmente activos.
La autorregulación se entiende como una forma de configurar, entre otros, pensamiento y afectos (Trías y Huerta, 2009). Es en la capacidad del «hacer», donde se produce la relación con la educación. Este hacer es posible gracias a la dimensión social presente en la vida del ser humano; aquí se enraíza la formación ciudadana. No se nace siendo ciudadano, sino que se aprende a ser ciudadano.
SEGREGACIÓN, JUSTICIA E INCLUSIÓN EN LOS SISTEMAS EDUCATIVOS CHILENO Y URUGUAYO
Authors: Haretche Olivera Carmen 
Published: October 2021

Se identifican políticas educativas que plausiblemente contribuyan a comprender las diferencias en la segregación, justicia e inclusión observada al finalizar la educación primaria en Chile y Uruguay. Ambos casos fueron seleccionados siguiendo el método de comparar sistemas similares.
Se utilizan los resultados en matemática de los estudiantes de 6to de primaria revelados en SERCE. Los hallazgos indican que, a pesar de que Chile parte de una mayor segregación sociocultural entre escuelas que Uruguay, la equidad en los desempeños educativos es menor en Uruguay que en Chile. En Chile se observan injusticias sobre las niñas en relación con los varones, mientras que en Uruguay las injusticias según el origen socioeconómico superan ampliamente la magnitud de las anteriores. En ambos países, el contexto sociocultural de los centros educativos es un factor de injusticia. Diferencias en el tipo de gobernanza, política curricular y evaluación docente, pueden contribuir a comprender los resultados.
APRENDIZAJE SOCIAL EN BEBÉS DE CERO A TRES AÑOS: INCLUIR LA NOCIÓN DE APRENDIZAJE EN UNA GUARDERÍA EN LA BRETAÑA, FRANCIA
Authors: Tirado Torres Jimena 
Published: October 2021

La etapa de la primera infancia es el período del ser humano que va del nacimiento a los ocho años; constituye un momento único del crecimiento en que el cerebro se desarrolla notablemente. La autora presenta hallazgos sobre la desconfianza que existe en las guarderías, en Francia, respecto a la noción de aprendizaje en la etapa de los cero a los tres años y señala como contraparte evidencias científicas que incluyen a Andrew N. Meltzoff, Anne-Sophie Rochegude y Louise Michaud, así como pautas por parte de la UNESCO, la OCDE, leyes y decretos particulares franceses que sustentan la pertinencia del aprendizaje en esa etapa. Se sustenta que el aprendizaje social o vicario es a través del cual los bebés aprenden, simplemente observando la conducta de un modelo social. La imitación demuestra probablemente uno de los efectos más largos en el aprendizaje y el comportamiento en la primera infancia.
Por último, la autora realiza propuestas para incluir la noción de aprendizaje en el proyecto educativo y proyecto pedagógico en una guardería al norte de Francia, sin que resulte ambicioso o sea limitante en la libre exploración de los pequeños.