bluuebunny/crossref_metadata_2025_split huggingface dataset:
The agricultural waste utilization for exploration of the extracellular enzyme potential of three novel white-rot Basidiomycetes: Fomes fomentarius TMF2, Schizophyllum commune TMF3 and Bjerkandera adusta TMF1
Authors: Ilić Nevena , Davidović Slađana , Milić Marija , Rajilić-Stojanović Mirjana , Pecarski Danijela , Ivančić-Šantek Mirela , Mihajlovski Katarina , Dimitrijević-Branković Suzana 
Published: April 2021

Abstract
Purpose This study aimed in exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential, covered by the circular economy frame.Methods A standard microbiological methods for the isolation of the white-rot fungal mycelia were used, followed by DNA extraction, PCR amplification, and the API ZYM test. The determination of laccase activity was based on the oxidation of guaiacol while the DNS method was used for the hydrolase determination.Results The isolates, belonged to Basidiomycetes, Fomes fomentraius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for Carboxymethyl cellulase and Avicelase production. The strain TMF1 was able to produce amylase during its growth on brewerʼs spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by TMF1 and S. commune TMF3. While growing on brewerʼs spent grain, TMF1 and TMF3 strains produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species.Conclusion The obtained results showed that newly isolates of white-rot fungi can grow on unexploited lignocellulosic waste materials to produce different enzymes as a value-added products suitable for various biotechnological applications.
Self-Healing Ability of Epoxy Vitrimer Nanocomposites Containing Bio-Based Curing Agents and Carbon Nanotubes for Corrosion Protection
Authors: Lorwanishpaisarn Narubeth , Srik Natwat , Jetsrisuparb Kaewta , Knijnenburg Jesper T.N. , Theerakulpisut Somnuk , Okhawilai Manunya , Kasemsiri Pornnapa 
Published: April 2021

Abstract
Epoxy is extensively used for anti-corrosion coatings on metallic materials. Conventional epoxy coatings have a permanent crosslink network that is unable to repair itself when cracks and damages occur on the coating layer. This study aims to develop self-healing epoxy vitrimer/carbon nanotube (CNTs) nanocomposite for coating. Two bio-based curing agents viz., cashew nut shell liquid (CNSL) and citric acid (CA) were employed to create covalent adaptable networks. The 0-0.5 wt% CNTs were also incorporated into epoxy/CNSL/CA matrix (V-CNT0-0.5). Based on the results of our study, thermomechanical properties of V-CNT nanocomposites increased with increasing CNTs content. The bond exchange reaction of esterification was thermally activated by near infrared (NIR) light. The V-CNT0.5 showed the highest self-healing efficiency in Shore D hardness of 97.37%. The corrosion resistance of coated steel with V-CNT0 and V-CNT0.5 were observed after immersing the samples in 3.5 wt% NaCl for 7 days. The corrosion rate of coated steel with V-CNT0.5 decreased from 9.53 x 102 MPY to 3.12 x 10-5 MPY whereas an increase in protection efficiency of 99.99% was observed. By taking advantages of the superior self-healing and anti-corrosion properties, V-CNT0.5 could prove to be a desirable organic anti-corrosion coating material.
Overcoming Reproductive and Psychological Concerns of Breast Cancer Survivors: A Randomized Controlled Trial
Authors: Barjasteh Samira , Farnam Farnaz , Nedjat Saharnaz , Elsous Aymen M.A. , Khakbazan Zohreh 
Published: April 2021

Abstract
Background: After developing breast cancer, women experience changes in their sexuality, femininity, and fertility. These changes lead to poor mental health and increased psychological stress. Therefore, this study aimed to investigate the effects of Good Enough Sex (GES)-based, couple-centered group counseling on reproductive and sexual concerns of breast cancer survivors.Methods: This was a quantitative randomized controlled clinical trial (RCT). After completing the informed consent forms, 100 women were assigned to the intervention and control groups (50 individuals per group) using randomized block design. The intervention included four 90-120-minute sexual counseling sessions with 2 and 3-month follow-ups. The data were collected using the socio-demographic and clinical characteristics, Persian version of Depression, Anxiety and Stress Scale (DASS-21), Reproductive Concerns After Cancer (RCAC) scale, and Female Sexual Function Index adaptation for Breast Cancer patients (FSFI-BC). The obtained data were analyzed in SPSS 20 using descriptive and repeated measures analysis of variance (ANOVA) test.Results: Significant differences were observed between the intervention and control groups in terms of the mean stress, anxiety, depression, reproductive concerns, and sexual function scores of the participants (P < 0.001). Therefore, the GES-based, couple-centered group counseling is associated with significant reductions in reproductive and sexual concerns of female breast cancer survivors.Conclusions: The designed psychosocial training and counseling intervention effectively reduced reproductive and sexual concerns of female breast cancer survivors. Therefore, these training and counseling programs can be organized by relevant service centers to promote the reproductive health of women with breast cancer.
The characteristics and effectiveness of pregnancy yoga interventions: A systematic review and meta-analysis
Authors: Corrigan Lisa , Moran Patrick , McGrath Niamh , Eustace-Cook Jessica , Daly Deirdre 
Published: April 2021

Abstract
Background: Yoga is a popular mind-body medicine frequently recommended and to pregnant women. Gaps remain in our understanding of core components of effective pregnancy yoga programmes. This review and meta-analysis examined the characteristics and effectiveness of pregnancy yoga, incorporating the FITT (frequency, intensity, time/duration and type) principle of exercise prescription in the analysis. Methods: The following electronic databases were searched: MEDLINE, PsycINFO, EMBASE, CINAHL, WHOLiS, AMED, ScieLo, ASSIA and Web of Science. Randomised control trials and quasi-experimental studies examining pregnancy yoga interventions were eligible for inclusion. Covidence was used to screen titles, abstracts, and full text articles. Outcomes of interest were stress, anxiety, depression, quality of life, labour duration, pain management in labour and mode of birth. The Cochrane Collaboration’s Risk of Bias Assessment tool was used to assess methodological quality of studies and GRADE criteria (Gradepro) evaluated quality of the evidence. Meta-analysis was performed using Revman 5.3. Results: 678 citations were retrieved, 24 studies met inclusion criteria. 22 studies with 1826 pregnant women were included for meta-analysis. Applying per-protocol analysis and a random effects model, the pooled standardised mean difference (SMD) for depression (-0.53; 95% CI: -1.04 to -0.02, P=0.04), anxiety (-0.82; 95% CI: -1.64 to -0.01; p=0.05), perceived stress (-1.03; 95% CI: -1.55 to -0.52; p<0.0001) and physiological stress (-0.24; 95% CI: -0.52 to 0.04, P=0.09) supported a statistically significant beneficial effect of pregnancy yoga interventions for anxiety, depression and perceived stress. Duration of labour was shorter (MD= -116.96; 95% CI -163.36 to -70.56, P<0.00001) and normal vaginal birth rates were higher in the yoga group (OR 2.72; 95% CI 1.26-5.90, p=0.01). The quality of evidence (GRADE) was low to very low for all factors. Twelve or more yoga sessions delivered weekly/bi-weekly had a statistically significant impact on mode of birth, while twelve or more yoga sessions of long duration (>60mins) had a statistically significant impact on perceived stress. Yoga sessions had a statistically significant impact on anxiety while yoga therapy had a statistically significant impact on depressionConclusion: The evidence supports previously cited positive effects of pregnancy yoga on anxiety, depression, perceived stress, normal vaginal birth and shorter duration of labour. Systematic review registration: PROSPERO, CRD42019119916. Registered on 11th January 2019.Protocol publication: Corrigan L, Eustace-Cook J, Moran P and Daly D. The effectiveness and characteristics of pregnancy yoga interventions: a systematic review protocol [version 2; peer review: 2 approved]. HRB Open Res 2020, 2:33 (https://doi.org/10.12688/hrbopenres.12967.2)
Modulation of lysosomal function as a therapeutic approach for coronaviral infections
Authors: Liu Yuan , Lear Travis , Larsen Mads , Lin Bo , Cao Qing , Alfaras Irene , Kennerdell Jason , Salminen Laura , Camarco Daniel , Lockwood Karina , Ma Jing , Liu Jie , Tan Jay , Myerburg Michael , Chen Yanwen , Croix Claudette St , Sekine Yusuke , Evankovich John , Finkel Toren , Chen Bill 
Published: April 2021

Abstract

The endo-lysosomal pathway plays an important role in pathogen clearance and both bacteria and viruses have evolved complex mechanisms to evade this host system. Here, we describe a novel aspect of coronaviral infection, whereby the master transcriptional regulator of lysosome biogenesis – TFEB – is targeted for proteasomal-mediated degradation upon viral infection. Through mass spectrometry analysis and an unbiased siRNA screen, we identify that TFEB protein stability is coordinately regulated by the E3 ubiquitin ligase subunit DCAF7 and the PAK2 kinase. In particular, viral infection triggers marked PAK2 activation, which in turn, phosphorylates and primes TFEB for ubiquitin-mediated protein degradation. Deletion of either DCAF7 or PAK2 blocks viral-mediated TFEB degradation and protects against viral-induced cytopathic effects. We further derive a series of small molecules that interfere with the DCAF7-TFEB interaction. These agents inhibit viral-triggered TFEB degradation and demonstrate broad anti-viral activities including attenuating in vivo SARS-CoV-2 infection. Together, these results delineate a viral-triggered pathway that disables the endogenous cellular system that maintains lysosomal function and suggest that small molecule inhibitors of the E3 ubiquitin ligase DCAF7 represent a novel class of endo-lysosomal, host-directed, anti-viral therapies.
Dynamic empirical research on user perception behavior of mobile library apps
Authors: Guo Caiqiang , Ming Junren 
Published: April 2021

Abstract
This paper aims to explore the influencing factors and differences between user perception behavior of mobile library apps at two different points in time. Based on TAM, we constructed the research model.We designed and distributed questionnaires to the users of mobile library apps at an interval of three months to collect dynamic data. A structural equation modeling approach was applied for analysis.The empirical results reveal that PEOU, PU, SI, and FC affect behavior intention, but the impact is different at different times. With users’ usage time increasing, TAM is still universal for user perception behavior of mobile library apps; user behavior has dynamic differences; and there are dynamic differences in users’ behavior intention to use mobile library apps among different groups. Dynamically tracking and comparing user perception behavior at two different times can help guide the library to improve the quality of mobile library services and reduce user churn.
Utilization of cajuput (Melaleuca leucadendron) twigs and sugarcane (Saccharum officinarum) bagasse agricultural waste for cellulose acetate production by environmentally friendly approach
Authors: Maryana Roni , Muryanto Muryanto , Triwahyuni Eka , Oktaviani Oktaviani , Prasetia Hafiizh , Das Atanu Kumar , Sudiyani Yanni 
Published: April 2021

Abstract
This study was carried out to investigate the extraction of cellulose acetate (CA) from cajuput (Melaleuca leucadendron) twigs and sugarcane (Saccharum officinarum) bagasse using an environmentally friendly method. At first, cellulose was extracted from cajuput twigs (CT) and sugarcane bagasse (SB) through prehydrolysis followed by soda (NaOH) pulping and elementary chlorine-free (ECF) bleaching. Later, the extracted cellulose was acetylated using iodine (I) as a catalyst. The obtained CA was characterized by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), thermal gravimetric analysis (TGA), scanning electron microscope (SEM) and X-ray diffraction. FTIR and NMR analysis proved the replacement of free OH (hydroxyl) groups by acetyl groups. The degree of substitution (DS) showed the acetylation capability of cellulose extracted from CT and SB as well. The cellulose diameter and its crystallinity index were measured by SEM and X-ray diffraction, respectively. Furthermore, the thermal gravimetric analysis showed that CA extracted from CT and SB was thermal resistance. Therefore, CT and SB could be potential alternative resources for CA production using the mentioned method.
A tough endothelium-like dressing for vascular stents
Authors: Chen Yin , Gao Peng , Huang Lu , Tan Xing , Zhou Ningling , Yang Tong , Qiu Hua , Dai Xin , Michael Sean , Tu Qiufen , Huang Nan , Guo Zhihong , Zhou Jianhua , Yang Zhilu , Wu Hongkai 
Published: April 2021

Abstract
Vascular stent is viewed as one of the greatest advancements in interventional cardiology. However, current approved stents suffer from in-stent restenosis associated with neointimal hyperplasia or stent thrombosis. To address this issue, we developed an endothelium-like (EL) dressing for vascular stents inspired by the importance and biological functions of native endothelium for cardiovascular system. Our EL dressing is based on a de novo designed hydrogel that is mechanically tough and could preserve integrity on stents during angioplasty. Due to its physiochemical similarities to subendothelial extracellular matrix, the EL dressing facilitated the adhesion and growth of endothelial cells. Besides, it is non-thrombotic and capable of inhibiting smooth muscle cells thanks to the capacity to catalyze nitric oxide generation. Transcriptome analysis further unraveled the EL dressing could modulate the inflammatory response and induce the relaxation of smooth muscle cells, while potentially promoting angiogenesis by stimulating the expression of angiogenic factors. In vivo study demonstrated vascular stents encapsulated by it promoted rapid restoration of native endothelium and persistently suppressed in-stent restenosis in both leporine and swine models. We expect such EL dressing will open a new avenue to the surface engineering of vascular implants for better clinical outcomes.
BPA and BPS Affect the Expression of Anti-Mullerian Hormone (AMH) and Its Receptor During Bovine Oocyte Maturation and Early Embryo Development
Authors: Saleh Angela Christina , Sabry Reem , Mastromonaco Gabriela Fabiana , Favetta Laura Alessandra 
Published: April 2021

Abstract
Background
Exposure to endocrine-disrupting chemicals, such as Bisphenol A (BPA) and Bisphenol S (BPS), is widespread and has negative implications on embryonic development. Preliminary evidence revealed that in women undergoing IVF treatment, urinary BPA levels were associated with low serum anti-Mullerian hormone, however a definitive relationship between the two has not yet been characterized.
Methods
This study aimed to evaluate BPA and BPS effects on in vitro oocyte maturation and early preimplantation embryo development through i) analysis of anti-Mullerian hormone (AMH) and anti-Mullerian hormone receptor II (AMHRII), ii) investigation of developmental parameters, such as cleavage, blastocyst rates and developmental arrest, iii) detection of apoptosis and iv) assessment of possible sex ratio skew. An in vitro bovine model was used as a translational model for human early embryonic development. We first assessed AMH and AMHRII levels after bisphenol exposure during oocyte maturation. Zygotes were also analyzed during cleavage and blastocysts stages. Techniques used include in vitro fertilization, quantitative polymerase chain reaction (qPCR), western blotting, TUNEL and immunofluorescence.
Results
Our findings show that BPA significantly decreased cleavage (p < 0.001), blastocyst (p < 0.005) and overall developmental rates as well as significantly increased embryonic arrest at the 2–4 cell stage (p < 0.05). Additionally, both BPA and BPS significantly increased DNA fragmentation in 2–4 cells, 8–16 cells and blastocyst embryos (p < 0.05). Furthermore, BPA and BPS alter AMH and AMHRII at the mRNA and protein level in both oocytes and blastocysts. BPA, but not BPS, also significantly skews sex ratios towards female blastocysts (p < 0.05)
Conclusion
This study shows that BPA affects AMH and AMHRII expression during oocyte maturation and that BPS exerts its effects to a greater extent after fertilization and therefore may not be a safer alternative to BPA. Our data lay the foundation for future functional studies, such as receptor kinetics, downstream effectors, and promoter activation/inhibition to prove a functional relationship between bisphenols and the AMH signalling system.
Septic Shock: Phenotypes and Outcomes
Authors: Cereuil Alexandre , Ronflé Romain , Culver Aurélien , Boucekine Mohamed , Papazian Laurent , Lefebvre Laurent , Leone Marc 
Published: April 2021

Abstract
Our goal was to determine the relevant variables and patient phenotypes to predict sepsis outcomes. We performed an ancillary study concerning 119 patients from three intensive care units (ICUs) in septic shock at ICU admittance (T0). We defined clinical worsening as having an increased Sequential Organ Failure Assessment (SOFA) score ≥ 1 48 hours after admission (ΔSOFA ≥ 1). We performed univariate and multivariate analyses according to 28-day mortality rate and ΔSOFA ≥ 1, and determined three patient phenotypes: safe, intermediate and poor. Persistence of the intermediate and poor phenotypes after T0 were defined as poor outcomes. At T0, the multivariate analysis showed two variables associated with 28-day mortality rate: norepinephrine dose and serum lactate concentration. Regarding ΔSOFA ≥ 1, we identified three variables at T0: norepinephrine dose, serum lactate concentration and venous-to-arterial carbon dioxide partial pressure difference (P(v-a)CO2). At T0, the three phenotypes (safe, intermediate, and poor) were distributed in 28 (24%), 70 (59%) and 21 (18%) patients, respectively. We thus suggested an algorithm featuring norepinephrine dose, serum lactate concentration and P(v-a)CO2 to predict patient outcomes and obtained an area under the curve (AUC) of 74% (63–85%). In conclusion, our findings underline that identifying relevant variables and phenotypes may help physicians recognize patient outcomes.
Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
Authors: Stadler Julia , Ade Julia , Hermanns Walter , Ritzmann Mathias , Wentzel Sarah , Hoelzle Katharina , Hoelzle Ludwig E. 
Published: April 2021

Abstract
Background Mycoplasma suis belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques. However, details on M. suis induced lesions as well as the distribution of M. suis in different organs are still lacking. Therefore, seven splenectomised pigs were experimentally infected and clinical and laboratory investigations as well as a detailed histopathological examination were performed. Detection and quantification of M. suis DNA in blood and various tissue samples was done using a quantitative real-time PCR.Results During the course of experimental infection, periodically occurring signs of infectious anaemia of pigs including severe icteroanaemia, fever, apathy and anorexia were observed. In addition, dermatological manifestations such as haemorrhagic diathesis presenting as petechiae occurred. The most important haematological alterations were normochromic, normocytic anaemia, hypoglycaemia as well as increased bilirubin and urea concentrations. Necropsy revealed predominant evidence of haemolysis with consecutive anaemia, as well as disseminated intravascular coagulation. M. suis was found in all investigated tissues with the highest copy numbers found in the kidneys. In Giemsa stained sections M. suis was only detected red blood cell (RBC)-associated.Conclusion In the present study, no RBC independent sequestration of M. suis was detected in organs of experimentally infected pigs. Pathological findings are most likely resulting from haemolysis, consecutive anaemia as well as from disseminated intravascular coagulation and subsequent organ impairments.
Smooth Border Between Inner Nuclear/Outer Plexiform Layers: Branch Retinal Vein Occlusion Biomarker of Fewer Macular Edema Recurrences
Authors: Sasajima Hirofumi , Tsuboi Kotaro , Kiyosawa Rokuki , Fukutomi Akira , Murotani Kenta , Kamei Motohiro 
Published: April 2021

Abstract
Purpose: To determine if the status of the border between the inner nuclear layer (INL) and outer plexiform layer (OPL) is associated with the number of macular edema (ME) recurrences secondary to branch retinal vein occlusion (BRVO).Methods: Thirty-seven consecutive eyes with BRVO treated with anti-vascular endothelial growth factor (VEGF) drugs were included. In optical coherence tomography (OCT) images at the initial visit, the border between the INL and OPL within the 1.5-mm vertical line from the fovea was manually traced using ImageJ software. The jagged ratio (JR), i.e., the border length divided by the spline curve length of the border was calculated using the “Spline fit” mode in the software.Results: The mean JR was 1.1± 0.07 (range, 1.01-1.27). The JR was correlated significantly with the number of anti-VEGF injections/year (P<0.0001, r=0.721). Among 37 eyes, nine (24.3%) received two or fewer injections/year, and their JRs were less than 1.05. The multivariate linear regression analysis showed that only the JR was correlated significantly with the number of anti-VEGF injections/year (P<0.0001). Conclusions: A smooth border between the INL and the OPL on OCT images at the initial visit may be a biomarker for fewer ME recurrences in eyes with BRVO.
4D volcanic geology of Hachijo-jima islet, Izu-Bonin arc
Authors: Osozawa Soichi , Ito Hisatoshi , Nakazato Hiroomi , Wakabayashi John 
Published: April 2021

Abstract
This study provides geological, geochemical, and chronological insight into the evolution of the Hachijo-jima volcanoes of the Izu Bonin arc. The regional Ata-Torihama tephra (Ata-Th; 0.24 Ma) and Kikai-Tozurahara tephra (K-Tz; 0.095 Ma) from Kyushu are intercalated within the voluminous proximal volcanic products. Our study combines detailed geologic mapping, tephrochronology, U-Pb zircon dating, and published drill core data from NEDO (1993) to evaluate the time transgressive 3-dimensional (thus 4-dimensional) structure of the dissected Mihara-yama volcano, the older of the two volcanoes on Hachijo-jima. The volcanic succession comprises terrestrial volcanic basement, marine volcaniclastic rocks, and terrestrial tuff intercalated with tholeiitic basalt and the regional calc-alkaline tephra layers. The un-dissected Hachijo-Fuji stratovolcano (tholeiitic basalt) overlies marine volcaniclastics abutting the northwestern paleo sea cliffs of Mihara-yama. Newly-described folding and normal faulting of the marine and overlying terrestrial volcaniclastic rocks suggests NW-SE shortening that may be associated with the collision of the Izu-Bonin arc with the Honshu arc. The proto Hachijo-jima volcano emerged above the sea > 0.24 Ma, and this date can be applied as a molecular biological calibration date for organisms on this island.