bluuebunny/crossref_metadata_2025_split huggingface dataset:
IN VITRO AND IN SILICO ALPHA-AMYLASE INHIBITION POTENTIAL (ANTI-DIABETIC ACTIVITY) OF PSEUDERANTHEMUM BICOLOR (SIMS) RADIK
Authors: BS RAMESH , RAVI LOKESH 
Published: January 2021

Objective: Aim of this study is to evaluate theanti-diabetic activity of Pseuderanthemum bicolor commonly called limang-sugat by inhibiting alpha-amylase protein.
Methods: Leaves of P. bicolor were extracted with methanol, chloroform, and ethyl acetate. The extracts were subjected for alpha-amylase inhibition assay and gas chromatography–mass spectrometry (GC–MS) analysis. Phytochemical compounds identified by GC-MS were subjected for protein-ligand docking study against alpha-amylase protein. Acarbose was used as a positive standard drug.
Results: The major bioactive compounds obtained from methanol, chloroform, and ethyl acetate extracts were 1,6;2,3-Dianhydro-4-Deoxy-Beta-D-Ribo-Hexopyranose, Pseduosarsasapogenin-5,20-Dien, methyl ether/Hexatriacontane, Di-N-decylsulfone/Octadecanal, and squalene, respectively. A total of 19 secondary metabolites were subjected for protein–ligand docking study against the alpha-amylase protein. The reference drug acarbose demonstrated binding energy of −7.8 Kcal/mol and formed 20 hydrogen bonds with the enzyme. Acarbose signified high polar interaction with the amylase enzyme. Among the 19 test ligands, “2,2-Dibromocholestanone” from ethyl acetate extract exemplified the highest binding energy of −9.3 Kcal/mol. The next highest remarkable inhibition was showed by “Pseduosarsasapogenin-5,20-Dien Methyl Ether” present in the methanol extract, with a binding energy of -9.3 Kcal/mol with the formation of 2 hydrogen bonds.
Conclusion: From the result, it could be concluded that the P. bicolor leaves contain various bioactive compounds which are considered as a good anti-diabetic drug.
COMPARATIVE STUDY ON RAMIPRIL AND TELMISARTAN-ASSOCIATED HYPONATREMIA
Authors: S BHUVANESHWARI , D VIJAYA 
Published: January 2021

Objectives: This study was planned to study the association of ramipril and telmisartan with hyponatremia and to compare the hyponatremia effect of ramipril and telmisartan.
Methods: The study was conducted in a tertiary care hospital. Serum sodium levels were assayed in patients taking ramipril and telmisartan. Fifty-one patients were recruited. The patient’s age, gender, drug dosage, and frequency of drug administration were collected using a pro forma. Statistical analysis of data was performed using SPSS version 23.0.
Results: About 35.3% (28 out of 51) of the study population administered with ramipril and telmisartan developed hyponatremia. Predisposition to develop hyponatremia was high in males compared to females. Incidence of hyponatremia was 43.8% (7 out of 16) in the age group of 50–60 years. Although, incidence of hyponatremia was 56.5% (13 out of 23) in ramipril group compared to 17.9% (5 out of 28) in telmisartan group, it was not statistically significant.
Conclusion: The current study laid emphasis on the requisite for monitoring of serum sodium level in patients on ramipril and telmisartan administration to avoid morbidity and mortality due to unexpected adverse reactions.
Residuos sólidos y su percepción social en el área terrestre de la isla Uvita, Limón, Costa Rica.
Authors: Luna Jiménez Miguel Andrés , Pereira Chaves José Miguel 
Published: January 2021

Esta investigación se realizó en la isla Uvita, cuyo objetivo fue identificar los residuos sólidos que se producen en isla Uvita, así como conocer la percepción social que se tiene de la misma, dicha Isla se ubica en el cantón central de provincia de Limón, dentro de las coordenadas latitud norte: 09o 59’ 43.9” N y 083o 00’ 46.2” O, sur: 09o 59’ 29.5” N y 083o 00’ 40.5”O, longitud oeste: 09o 59’ 39.7” N y 083o 00’ 48.0”O, este: 09o 59’ 41.1” N y 083o 00’ 38.1”O9 durante el periodo comprendido entre noviembre 2016 a mayo del 2017, donde se ha interpretado el residuo sólido como la parte no utilizable o consumible de algún producto o mercadería proveniente de la actividad humana, la metodología empleada fue una variación a la metodología descrita en el decreto ejecutivo N0 37745-S correspondiente al Estudio, Generación y Composición de Residuos Sólidos Ordinarios, entre los principales resultados obtenidos se documentó un total de 519,3 kg recolectados durante dicho periodo de investigación, entre los diversos residuos sólidos estaban plástico, vidrio, papel y cartón, polilaminados y diversos objetos que no entraban en las categorías anteriores clasificados como “otros” para efectos de la presente investigación; la percepción de los visitantes dio a conocer la necesidad de la implementación de una estrategia de manejo de los residuos sólidos que cuente con la vinculación de sectores sociales e instituciones claves como la Municipalidad de Limón.
Necrosis Pancreatic Infectious Virus does not block 701-STAT1 (α/β) tyrosine in Oncorhynchus mykiss (‎Salmoniformes: ‎Salmonidae).
Authors: Cortés Donald Arguedas 
Published: January 2021

ABSTRACT: Necrosis Pancreatic Infectious Virus does not block 701-STAT1 (α/β) tyrosine in Oncorhynchus mykiss (‎Salmoniformes: ‎Salmonidae).
Introduction: Infectious pancreatic necrosis virus (IPNV) is a pathogen important that affects predominantly salmonids. The type I interferon alpha system has a crucial role in the first line of defense against IPNV infection. IFN-I(α) activation triggers the signaling pathway JAK-STAT, binding to their receptors results in the rapid phosphorylation of STATs a critical step for the nuclear translocation to induce the interferon stimulated genes (ISGs). The relationship between infectivity level of IPNV strain and pathway signaling of IFN is yet poorly understood. Objective: our purpose was to investigate if the IFN-I(α) signaling pathway is affected by IPNV strains of different infectivity levels.
Methods: We used two IPNV isolated (VR-299 and Sp) to infect RTG-2 cells. Total RNA was isolated using the commercial kit for determine to VP2 expression and ISGs using qRT-PCR. Western Immunoblotting analysis was carried out for determine the 701 STAT1(α/β) phosphorylation into infected cells.
Results: Hence, a higher virulence strain is not associated with a greater blocking effect for interferon signaling. Furthermore, the activation of Y701-STAT1 (α/β) was significantly increased in serotype Sp virus infected cells compared with serotype VR-299 virus infected cells, indicating that IPNV inhibits IFN signaling pathway.
Conclusions: As concluded, IPNV does not block the phosphorylation of 701-tyrosine STAT1 (α/β) stimulated by IFN-I(α), contrary to other RNA viruses.
Evaluación de tres productos desinfectantes sobre semillas de maíz y cebada para la producción en la tecnología de Forraje Verde Hidropónico
Authors: Zuñiga Orozco Andres , Beauregard Zúñiga Ivette 
Published: January 2021

Se analizó variables de crecimiento utilizando tratamientos desinfectantes de semillas, en diferentes dosis y en dos forrajes comúnmente usados para alimentación animal (maíz y cebada), haciendo uso de la tecnología FVH en la localidad de San Carlos, Alajuela, Costa Rica. Se usó los productos Kilol® (L DF-100 11 SL), Cloro (4%) y Agua oxigenada (10%) a una dosis 7.5 ml/L y 5 ml/L, en dos tipos de forraje y se evaluaron las variables: producción de biomasa neta, materia seca (MS), proteína cruda e incidencia de hongos. En cuanto a los resultados el mejor tratamiento para control de hongos fue el T6 (Agua oxigenada a 5ml/L) para cebada, en maíz no hubo diferencias. En producción de biomasa neta, el maíz acumuló mayor biomasa que cebada, siendo el T5 y T6 los que obtuvieron mayor peso y % de proteína. En cuanto al contenido de materia seca, hay un efecto contraproducente al aplicar desinfectantes, puesto que todos acumularon menos materia seca con respecto al testigo, posiblemente debido a un efecto fitotóxico. La proteína cruda y materia seca en la cebada, supera el contenido de un alimento comercial para alimentación animal, el kilo de cebada es 3.85 veces más barato. Finalmente, se concluye que usar agua oxigenada a 5ml/L es la mejor opción para desinfección de semilla, incluso con un efecto positivo al promover germinación, crecimiento y acumulación de biomasa neta. En conclusión, todos los tratamientos tuvieron presencia de hongos saprófitos, pero el maíz fue el menos afectado y aunque en cebada se presentaron, no fueron especialmente importantes. El T6 (AO a 5 ml/L) fue quien mejor controló hongos en cebada. Tanto en maíz como en cebada los tratamientos que presentaron valores más altos en producción de biomasa neta fueron el T5 (AO a 7.5 ml/L) y T6 (AO a 5 ml/L). Tanto en maíz como en cebada, el tratamiento que presenta los valores más altos de materia seca fue el T7 (Testigo), pudo haber un efecto de fitotoxicidad en los demás tratamientos. Tanto en maíz como en cebada los tratamientos que presentaron valores más altos en proteína fueron el T5 (AO a 7.5 ml/L) en maíz y los T3, T5 y T6 en cebada. El agua oxigenada en dosis de 5 ml/L (T6), presentó los mejores efectos entre las variables analizadas y aunque en MS y proteína no fue así, se encuentra dentro de los valores recomendados para la alimentación animal, según diferentes autores. Además, presenta un efecto bioestimulante en germinación de semillas. Los resultados analizados en los FVH de esta investigación, demuestran tener mayor cantidad de proteína y materia seca que el alimento concentrado Vap Feed®.
El El desempeño de los Objetivos de Desarrollo Sostenible, COVID-19 y la Costa Rica Bicentenaria
Authors: Rojas Morales Jose Rodrigo , Montero-Miranda Eric , Campos-Calderón Fernando 
Published: January 2021

RESUMEN:
Costa Rica fue el primer país del mundo en comprometerse con el cumplimiento de los Objetivos de Desarrollo Sostenible (ODS). A cinco años de la adhesión voluntaria, luego de dos informes voluntario y en medio de la pandemia COVID-19, se efectúa un estudio sobre el desempeño de los ODS y los retos de cara a la Costa Rica bicentenaria. Para ello se recurre a un análisis de entorno PESTAL y a una matriz de evaluación de cumplimiento. Los resultados demuestran que el país mantiene avances en los objetivos 2, 3, 7, 12, 13, y 15; en tanto que existen rezagos en los ODS 1, 6, 8, 11, 14 y 16. Si bien el cumplimiento de los objetivos se ha visto comprometido por la pandemia, hay condiciones preexistentes que magnifican los pasivos y deudas ambientales. Además de medidas relacionadas con políticas monetarias y reactivación económica, se deben promover proyectos de ordenamiento territorial, movilidad eléctrica e independencia del petróleo, así como soluciones basadas en la naturaleza como hoja de ruta para la gobernanza y desempeño de los ODS
Linguagem natural ou linguagem controlada? Influência da palavra-chave na representação para indexação e recuperação de informações
Authors: Fujita Mariangela Spotti Lopes 
Published: January 2021

Trata-se de ensaio para apresentar o debate entre linguagem natural ou linguagem controlada na perspectiva contemporâneada internet e web 2.0 em que as atuais bases de dados, muitas de acesso aberto como os repositórios, são heterogêneasquanto à organização e representação da informação. O objetivo é investigar, por meio de revisão de literatura, o uso delinguagem natural ou de linguagem controlada por sistemas de informação, de modo geral, e por repositórios, de modomais específico, para observar, avaliar e discutir os resultados, recomendações e reflexões. Na análise qualitativa foramselecionados 90 trabalhos que, após leitura prévia, foram escolhidas 73 produções científicas. Na sequência, a leitura foiextensiva para coleta dos objetivos, metodologia, resultados, conclusões e principais pontos de vista dos autores. Umaamostra representativa de 38 textos está distribuída em 12 categorias de análise para facilitar a compreensão macro e microdesse debate e seus rumos. Os resultados demonstraram que investigadores ao longo dos anos, desde que surgiram asprimeiras bases de dados, continuam realizando estudos exploratórios para observar comportamentos de indexadores ede usuários, uso de ferramentas, processos, produtos e ambiente com diferentes contextos e tipologias textuais. Estudosde aplicação de novas metodologias, processos e produtos também são encontrados e demonstram novas possibilidadesde soluções conciliadoras. Concluiu-se que a coexistência entre linguagem natural e linguagem controlada é necessária emsistemas de informação para a representação na indexação e na busca.
Palavras-chave: Palavra-chave. Linguagem natural. Linguagem controlada. Vocabulário controlado.
Conceitos sobre Gestão do Conhecimento: uma revisão sistemática da literatura brasileira
Authors: Valentim Marta Ligia Pomim 
Published: January 2021

Apresenta-se os conceitos presentes na literatura brasileira sobre gestão do conhecimento e seus elementos constitutivos. A literatura internacional oferece uma quantidade significativa de textos que abordam as concepções, conceitos e definições sobre gestão do conhecimento, contudo, este artigo enfoca apenas a literatura produzida por pesquisadores brasileiros, publicada em periódicos nacionais, no intuito de conhecer o estado da arte sobre gestão do conhecimento e observar a evolução do conceito ao longo do tempo no País. Como procedimentos metodológicos, definiu-se a Revisão Sistemática de Literatura, aplicando-se um protocolo de pesquisa, de maneira a atender o rigor científico para a coleta e a análise dos dados. A partir da análise dos textos recuperados foi possível conhecer os conceitos atribuídos para gestão do conhecimento, bem como os elementos constitutivos mais significativos na visão dos pesquisadores brasileiros.

Palavras-chave: Gestão do conhecimento. Conceitos. Compartilhamento de conhecimento. Revisão Sistemática da Literatura. Brasil.
Apresenta-se os conceitos presentes na literatura brasileira sobre gestão do conhecimento e seus elementos constitutivos. A literatura internacional oferece uma quantidade significativa de textos que abordam as concepções, conceitos e definições sobre gestão do conhecimento, contudo, este artigo enfoca apenas a literatura produzida por pesquisadores brasileiros, publicada em periódicos nacionais, no intuito de conhecer o estado da arte sobre gestão do conhecimento e observar a evolução do conceito ao longo do tempo no País. Como procedimentos metodológicos, definiu-se a Revisão Sistemática de Literatura, aplicando-se um protocolo de pesquisa, de maneira a atender o rigor científico para a coleta e a análise dos dados. A partir da análise dos textos recuperados foi possível conhecer os conceitos atribuídos para gestão do conhecimento, bem como os elementos constitutivos mais significativos na visão dos pesquisadores brasileiros.
Palavras-chave: Gestão do conhecimento. Conceitos. Compartilhamento de conhecimento. Revisão Sistemática da Literatura. Brasil.
The informativity of the markers of iron metabolism in the differential diagnosis of anemia of inflammation in children with chronic HBV infection
Authors: Inoyatova F. I. , Ikramova N. A. , Inogamova G. Z. , Kadyrkhodzhayeva Kh. M. , Abdullayeva F. G. , Valiyeva N. K. , Akhmedova A. Kh. 
Published: January 2021

Objective: To assess diagnostic importance of iron metabolism markers in the progression of anemia of inflammation (AI) in children with chronic HBV infection.Materials and methods: Among 148 examined children with chronic HBV infection 140 had AI, 60.7% of them with refractory (RA) and 39.3% with non-refractory (nRA) progression variant. Complete blood count was performed using hematologic automatic analyzer. Virologic verification of HBV was done by ELISA and PCR. ELISA was used to determine 25-hepcidin, serum iron, ferritin, trasferrin, sTfR, IL-1, IL-6. The index sTfR/log10Ft was calculated.Results: Performing the examination of children with chronic HBV infection we determined high prevalence of AI, equal to 94.6%, which was characterized by normocyte normochromic progression, thrombocytopenia, thrombocrit decrease in case of RA, and microcyte hypochromic progression with erythrocyte anisocytosis in case of nRA. Despite the high inflammatory index induced by HBV viral replication, children with RA had characteristic decrease in 25-hepcidin and transferrin parameters with background high values of ferritin, while nRA was characterized by rise of 25-hepcidin and transferrin spectrum with low values of serum iron and ferritin.Conclusions. In the genesis of AI in chronic HBV cases two pathogenic variants were determined: true iron deficiency with ferromarkers in the type of IDA characteristic for nRA and redistribution iron deficiency compliant to hemosiderosis characteristic for RA. Priority in the differential diagnosis of AI variants is given to the comparison of sTfR/log10Ft index parameters (RA<1.0; nRA>2.0) with reference level of 25-hepcidin<28,68ng/ml in case of RA, and >56,37ng/ml in case of nRA.
The impact of viral infections on the results of hemopoietic stem cell transplantation in patients with hematologic malignancies
Authors: Antonova T. V. , Pobegalova O. E. , Nozhkin M. S. , Romanova M. A. , Gorchakova O. V. 
Published: January 2021

Study Objective: to assess the impact of herpesviruses infections reactivation and concomitant chronic hepatitis C infection (CHC) on complications and one-year survival after hemopoietic stem cell transplantation (HSCT) in patients with hematologic malignancies.Materials and Methods: medical records of HSCT recipients with PCR-confirmed viral infections (CMV, HHV-6, EBV, HSV-1,2, HCV) from Raisa Gorbacheva Memorial Research Institute for Pediatric Oncology, Hematology and Transplantation of Pavlov First St.Petersburg State Medical University were analyzed retrospectively. The following groups were composed: patients with herpesviruses infections reactivation (PCR+) without clinical manifestation (n=37), patients with clinically manifest herpesviruses infections (n=21), and patients with CHC (n=28). Control groups were selected using matched samples method from patients with negative test results. HSCT complications rate and one-year survival were compared. Statistical analysis was carried out using SPSS Statistics 22 software.Results: Herpesviruses infections reactivation was revealed in 61,2% of 343 patients. The complications rate across the groups did not differ significantly. One-year survival (Kaplan-Meier) was significantly lower in the groups with herpesviruses infections (PCR+) without clinical manifestation (52,1% vs 73,5%), manifest herpesviruses infections (38,1% vs 75,0%), and CHC (64,3% vs 92,9%) than in the respective control groups. There were no significant differences between the group with reactivation of herpesviruses infections without clinical manifestation and the group with manifest herpesviruses infections.Conclusion: Significant impact of herpesviruses infections, including those without clinical manifestation, and HCH with minor symptoms and normal liver functions on one-year survival in patients with hematologic malignancies justifies wider use of antiviral therapy in patients requiring HSCT.
The use of bicyclol in the treatment of severe liver fibrosis: experimental rationale
Authors: Zhdanov K. V. , Saulevich A. V. , Kozlov K. V. , Zakharenko S. M. , Sukachev V. S. , Karev V. E. , Zakharkiv Yu. F. , Potekhin I. V. , Mukhtarov R. M. , Kasianenko K. V. , Karyakin S. S. , Chernenok M. G. 
Published: January 2021

Aim. To evaluate the antifibrotic effect of bicyclol with promising clinical effects on diffuse parenchymal liver diseases.Materials and methods: We have included 40 adult male Wistar rats (weight, 180 to 200g) with experimentally inducted toxic F3 stage liver fibrosis. Control group consisted of 16 rats that received standart chow, experimental group of 24 rats also received 30-day bicyclol supply. To evaluate the results we used histological, biochemical, molecular genetic and statistical methods.Results. In experimental group interlobular fibrosis was maintained in 4,16% (p<0,001) of cases after the end of the therapy, stromal fibrosis of portal tracts was observed in 62,5% (p<0,001) of cases. Periportal fibrosis detection rate in experimental group was 66,7% (p<0,001). Rats from experimental group showed regression of liver fibrosis to F1- F2 stage in 16,6% (METAVIR) whereas control group had F3 stage liver fibrosis in 62,5% (p <0,05). Statistically significant increase in ALT activity and decreased total protein levels in control group were observed. No changes in gut microbiome profile in experimental animals were found.Conclusion. Use of bicyclol led to pronounced suppression of liver fibrotic changes in laboratory animals and temporary decreased serum ALT level. Our study showed no link between therapeutic effects of the drug and gut microbiome status.
Features of Surgical Treatment of Posttraumatic Lower Limbs of Different Lengths in Adult Patients
Authors: Artemiev A. A. , Ivashkin A. N. , Kashoob A. M. , Solovyov Yu. S. , Gululyan G. G. 
Published: January 2021

Relevance.Different lengths of the lower extremities have a negative impact on the position of the pelvis and spine and lead to secondary deformities. To prevent these consequences, early surgical correction of the shortening is necessary.Aim of study.Study of the features of post-traumatic shortening of the lower extremities, mechanisms of adaptation to this condition and, on the basis of this, optimization of surgical technique when performing reconstructive and restorative operations.Material and methods.A total of 276 patients with posttraumatic shortening of the lower extremities were examined, 102 of whom were operated on. Comparative radiography of both legs was used to diagnose different lengths. The Ilizarov method was used as the main method of surgical correction.Results.We studied the mechanisms of adaptation of patients to post-traumatic shortening of the hip and lower leg and optimized surgical technique. A method was developed for determining the optimal elongation value.Conclusion.The most effective and least traumatic method is external osteosynthesis according to Ilizarov. Distraction in the apparatus allows the formation of a regenerate of the required shape and length and the elongation of exactly the amount that is optimal for a given patient.