bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary tea tree (Melaleucae Aetheroleum) oil fortifies growth, biochemical, immune-antioxidant trait, gene function, tissue reaction, and Aeromonas sobria resistance in Nile tilapia (Oreochromis niloticus)
Authors: Shaalan Mohamed , Mahboub Heba H. , Abdelgawad Ahmed Hosny , Abdelwarith Abdelwahab A. , Younis Elsayed M. , Elnegiry Ahmed A. , Basher Asmaa W. , El-Houseiny Walaa , Shawky Sherif M. , Orabi Sahar H. , Davies Simon J. , Mahmoud Yasmina K. 
Published: January 2025

Abstract
The current study had aimed to assess the long-term dietary supplementation with Melaleucae aetheroleum, tea tree essential oil (TTO). The impact on growth performance, biochemical indices, immune function, oxidant/antioxidant activity, gene expression, histopathology, and resistance against Aeromonas sobria in Nile tilapia (Oreochromis niloticus) was investigated. Four groups (with five replicates; G1 (control group, G2, G3, and G4) of Nile tilapia received diets enriched with TTO (doses of 0.0, 0.5, 1.0, and 2.0 mL/kg diet) for 60 days, then fish were challenged by A. sobria. Outcomes indicated an extensive elevation in growth metrics (final body weight, weight gain, SGR, feed intake and fish body protein). Similarly, the total blood protein, albumin, total globulin levels, Serum complement-3 levels, lysozyme activity, immunoglobulin M (IgM), nitric oxide, and phagocytic activity were significantly enhanced in all treatments, notably in the 2.0 mL TTO/kg fed groups compared to the control. Lower levels of urea, creatinine, AST, ALP, ALT, cortisol, glucose, triglycerides, cholesterol and body crude lipids were observed in the fish that were fed a 2.0 mL TTO/kg diet. Supplementing TTO at 2.0-mL /kg diet revealed the best results for elevating CAT, SOD, and GSH activities plus declining MDA value in hepatic homogenate. Additionally, dietary 2.0-mL TTO/kg showed the best outcomes for the intestinal morphometry plus maintaining the histological picture in spleen and liver. Concurrently, fish that were fed a 2.0 mL TTO/kg diet exhibited a substantial upregulation of TNF-α, IL-1β, IL-10, TFG-β, IFN-γ and BCL-2 genes in the liver, while, caspase-3, and BAX were downregulated. Furthermore, TTO-enriched diets enhanced the relative percentage survival post-A. sobria challenge plus enhanced the clinical picture in a dose-dependent manner. Taken together, the findings revealed that long-term exposure to dietary TTO fortified the physiological performance, oxidant/anti-oxidant stability, immune function, gene expression, histological picture, and resistance of Nile tilapia against A. sobria.
HBV DNA integration and somatic mutations in HCC patients with HBV-HCV dual infection reveals profiles intermediate between HBV- and HCV-related HCC
Authors: Li Chiao-Ling , Hsu Chia-Lang , Lin You-Yu , Ho Ming-Chih , Hu Rey-Heng , Tzeng Sheng-Tai , Wang Ya-Chun , Tanaka Yasuhito , Chen Pei-Jer , Yeh Shiou-Hwei 
Published: January 2025

Abstract
Background
In regions with a high prevalence of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, coinfected patients face a heightened risk of developing hepatocellular carcinoma (HCC), termed HBV/HCV-related HCC (HBCV-HCC). We aimed to investigate the contribution of preexisting chronic hepatitis B (CHB) and subsequent chronic hepatitis C (CHC) to the development of HBCV-HCC.

Methods
We examined HBV’s involvement in 93 HBCV-HCC cases by analyzing HBV DNA integration as an indicator of HCC originating from HBV-infected hepatocytes, compared with 164 HBV-HCCs and 56 HCV-HCCs as controls.

Results
Next generation sequencing revealed that 55% of HBCV-HCCs exhibited clonal HBV integration, which falls between the rates observed in HBV-HCCs (88%) and HCV-HCCs (7%), with similar integration patterns to HBV-HCCs. Common HCC somatic mutation analysis indicated HCV superinfection in HBCV-HCCs correlated with increased mutation rates in the telomerase reverse transcriptase (TERT) promoter and beta-catenin genes. Transcriptome analysis showed a prevalence of replicating HCV over HBV in HBCV-HCCs, with preexisting HBV exerting a proliferative role. The comparison of clinical characteristics revealed similarities between HBCV-HCC and HCV-HCC patients, including later onset for HBCV-HCC, possibly due to HCV superinfection slowing carcinogenesis. Notably, HBCV-HCCs with the same driver mutation, HBV integration at the TERT promoter, tended to develop later and showed a better prognosis post-tumor resection than HBV-HCCs.

Conclusions
Our findings shed light on the interplay between preexisting CHB and subsequent CHC in elevating the risk of HBCV-HCC. These insights are crucial for understanding viral etiology-specific carcinogenesis and guiding surveillance policies for HBCV-HCC post-antiviral therapy.
Pan-cancer drivers of metastasis
Authors: Lusby Ryan , Demirdizen Engin , Inayatullah Mohammed , Kundu Paramita , Maiques Oscar , Zhang Ziyi , Terp Mikkel Green , Sanz-Moreno Victoria , Tiwari Vijay K. 
Published: January 2025

AbstractMetastasis remains a leading cause of cancer-related mortality, irrespective of the primary tumour origin. However, the core gene regulatory program governing distinct stages of metastasis across cancers remains poorly understood. We investigate this through single-cell transcriptome analysis encompassing over two hundred patients with metastatic and non-metastatic tumours across six cancer types. Our analysis revealed a prognostic core gene signature that provides insights into the intricate cellular dynamics and gene regulatory networks driving metastasis progression at the pan-cancer and single-cell level. Notably, the dissection of transcription factor networks active across different stages of metastasis, combined with functional perturbation, identified SP1 and KLF5 as key regulators, acting as drivers and suppressors of metastasis, respectively, at critical steps of this transition across multiple cancer types. Through in vivo and in vitro loss of function of SP1 in cancer cells, we revealed its role in driving cancer cell survival, invasive growth, and metastatic colonisation. Furthermore, tumour cells and the microenvironment increasingly engage in communication through WNT signalling as metastasis progresses, driven by SP1. Further validating these observations, a drug repurposing analysis identified distinct FDA-approved drugs with anti-metastasis properties, including inhibitors of WNT signalling across various cancers.
The evolution of health policy and systems research in 11 low- and middle-income countries and the role of the Alliance for Health Policy and Systems Research: a bibliometric analysis for 1999–2020
Authors: Paichadze Nino , Cook Emma K. , Rosen Heather E. , Kurtovic Sara , Hyder Adnan A. 
Published: January 2025

Abstract
Objectives
Given the rapid growth of the field of health policy and systems research (HPSR), it is important to monitor the research environment, especially the evolution of HPSR research outputs in low- and middle-income countries (LMICs). The objective of this study was to generate quantitative metrics to assess the production of HPSR publications and the role of the Alliance for Health Policy and Systems Research (the Alliance) grant-funded projects in 11 LMICs over the past 20 years.

Methods
We conducted a systematic literature search for HPSR literature from 1999 to 2020 pertaining to 11 target LMIC countries, including grey literature. We analysed the frequency of publications over time, by country and by thematic area. We then used a database of the Alliance’s previous grantees to analyse authorship by Alliance-funded investigators.

Results
HPSR publications across all 11 target countries increased steadily over the past two decades and the rate of publication increased by an average of 34% per year. The majority of HPSR publications during the study period were in health systems (23%) and health workforce (19%) thematic areas. Nineteen per cent of HPSR publications during this time were authored by Alliance-funded investigators. There was extensive heterogeneity between countries both in number of publications and in proportion of publications authored by Alliance-funded investigators.

Conclusions
Growth in the HPSR research environment reflects the expanding prominence of the HPSR field and increased HPSR research capacity in the 11 target countries. Alliance-funded investigators made an important contribution to the growth in HPSR output in these countries.
Associations between HEXACO personality traits, substance use disorders, and behavioral addictions: a protocol for a comprehensive systematic review and meta-analysis
Authors: Sharifibastan Farangis , Erevik Eilin Kristine , Morken Katharina Teresa Enehaug , Pallesen Ståle 
Published: January 2025

Abstract
Background
While research has explored the connection between addiction and personality, no systematic study has examined how substance use disorders (SUD) and behavioral addictions specifically relate to the HEXACO model of personality. This systematic review and meta-analysis aim to fill this gap by investigating the association between HEXACO personality traits and various addictions, including illegal substances (e.g., narcotics and cannabis) and behavioral addictions (e.g., gambling, gaming, social media addiction, and compulsive sexual behavior disorder) across different populations.

Methods
The protocol is in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) statement. Searches will be conducted in databases including APA PsycINFO (Ovid), MEDLINE (Ovid), ProQuest, Web of Science, CINAHL, Wiley Online Library, and Google Scholar. Empirical studies published as full papers in peer-reviewed journals or as full dissertations, in English, other European languages, or Persian, investigating the association between HEXACO personality traits and addictive disorders are eligible. Two reviewers will independently screen all citations and full-text articles, and extract data using the Covidence software. They will further assess the risk of bias and quality of the studies using the Newcastle–Ottawa Scale (NOS) for longitudinal and cohort studies, an adapted version of the NOS for cross-sectional studies. Publication bias will be evaluated using funnel plots, Egger’s test, and trim and fill analysis. In addition to a narrative summary, meta-analyses will be conducted if data are sufficient. Random effects models will be used to pool effect sizes. Subgroup analyses and meta-regression will be performed to investigate potential sources of heterogeneity. Sensitivity analyses will examine the robustness of the results. Data analysis will be conducted using Comprehensive Meta-Analysis (CMA), version 4.

Discussion
This review and meta-analysis will be the first to systematically explore and integrate the evidence available on the association between the HEXACO personality traits and SUD and behavioral addictions. By consolidating information, the study will enhance our understanding of the role of personality traits in the development, maintenance, and treatment of SUD and behavioral addictions.

Systematic review registration
PROSPERO CRD42023468153.
Mineral supplementation by artificial salt licks is comparatively effective as natural salt licks for Malaysian mammals
Authors: Razali Nor Bazilah , Mansor Mohammad Saiful , Farinordin Farah Ayuni , Zaini Mohd Izzat-Husna Ahmad , Razali Siti Hajar Atiqah , Patah Pazil Abdul , Husin Shahril Mod , Hussein Mohamed Shah Redza , Nor Shukor Md 
Published: January 2025

Abstract
Background
Mineral-rich licks are known to provide mineral supplements to wildlife species, including mammals. Artificial salt licks have been provided as habitat enrichment in protected areas and secondary forests in Peninsular Malaysia since 2012. However, few studies have investigated artificial salt licks, particularly their mineral composition and roles in different habitats. We used 40 camera traps to assess mammal species diversity and assemblages at 20 salt licks (nine natural and 11 artificial licks) in three forest reserves in Peninsular Malaysia. Nine mineral elements from water and soil samples from natural and artificial licks in primary and secondary forests were analysed.

Results
Twenty-nine mammal species were identified across salt licks, with 16 species being salt lick users, including eight herbivorous, five frugivorous and three carnivorous mammals, most of which moved in groups and solitarily. The mammal species assemblages across both salt lick and habitat types demonstrated a predominantly nested pattern, with herbivorous and frugivorous mammals being the primary users. The artificial salt licks in both habitats showcased the same feeding guilds including species that are resilient to habitat changes. The visitation frequency at both types of licks and habitats suggests that each salt lick user had its own preferences for minerals from salt lick water and soil.

Conclusions
This study suggests that artificial licks may be as effective as natural licks in providing minerals to many mammal species both in primary and secondary forests. Thus, they could be used to enrich degraded habitats. Enriching degraded habitats is essential for supporting the ecosystem, especially in carbon reduction, biodiversity conservation and connectivity between degraded forests and intact forests.
Nitrogen and water additions with or without mowing altered soil microbial community characteristics in a semi-arid steppe
Authors: Liu Hao , Ren Feirong , Wan Shiqiang , Han Shijie , Zheng Junqiang 
Published: January 2025

Abstract
Background
The temperate grasslands are facing numerous pressures from global change. Despite their essential ecological and economic role, how their microbial communities react to multiple varying factors remain obscure. In this study, we simulated three global change drivers, i.e., nitrogen deposition (ambient N vs. elevated N, aN vs. eN), precipitation increase (ambient precipitation vs. elevated precipitation, aP vs. eP), and mowing, represented experimentally by clipping (unclipped vs. clipped, uC vs. CL), together in all possible combinations in a temperate semi-arid grassland ecosystem.

Results
Nitrogen addition had negative effects on the richness of bacterial and fungal communties, significantly changed their structures (P < 0.05) and increased their dissimilarities (P < 0.05), while water addition had positive effects on fungal and protist communities and significantly stimulated the α-diversity of protist communities under N addition without clipping, which was in contrast to the effect in clipped plots. Clipping had a marginal effect on fungal communities and significantly affected protist communities (P < 0.05). A notable interactive effect of N and precipitation on the structure of bacterial communities and a significant interactive effect of clipping and precipitation on protists were found. Combination effects of N with precipitation or clipping on module aggregation of meta-networks were also observed between uC and CL, as well as aP and eP meta-networks. Bacterial, fungal, and protist communities varied in their assembly mechanisms, and their assembly processes differed in response to the three global change factors.

Conclusions
Overall, N, water addition, and clipping individually and/or interactively, in distinct degrees, altered soil microbial interaction, community structure, and the potential function in a semi-arid steppe. These findings enhance our understanding of soil microbial community assembly and provide a scientific basis for managing temperate grasslands, particularly in the context of global change's impact on ecosystem function and stability.
Toward an ecosystem of non-fungible tokens from mapping public opinions on social media
Authors: Xing Yunfei , Zhang Justin Z. , He Yuming , Li Yueqi 
Published: January 2025

AbstractAs blockchain technology advances, non-fungible tokens (NFTs) are emerging as unconventional assets in the commercial market. However, it is necessary to establish a comprehensive NFT ecosystem that addresses the prevailing public concerns. This study aimed to bridge this gap by analyzing user-generated content on prominent social media platforms such as Twitter, Weibo, and Reddit. Employing text clustering and topic modeling techniques, such as Latent Dirichlet Allocation, we constructed an analytical framework to delve into the intricacies of the NFT ecosystem. Our investigation revealed seven distinct topics from Twitter and Reddit data and eight topics from Weibo data. Weibo users predominantly engaged in reviews and critiques, whereas Twitter and Reddit users emphasized personal experiences and perceptions. The NFT ecosystem encompasses several crucial elements, including transactions, customers, infrastructure, products, environments, and perceptions. By identifying the prevailing trends and common issues, this study offers valuable guidance for the development of NFT ecosystems.
Liquidity constraints, real estate regulation, and local government debt risks
Authors: Gong Xiao-Li , Lu Jin-Yan , Xiong Xiong , Zhang Wei 
Published: January 2025

AbstractGiven the existence of real estate market bubbles and risks arising from high government debt, countries are faced with the challenge of preventing systemic risks. This study investigates the macroeconomic dynamics of the real estate market and local government debt risk from the perspective of liquidity constraints. We build a dynamic stochastic general equilibrium model with real estate and local government debt risk based on the New Keynesian-Dynamic Stochastic General Equilibrium Model (NK-DSGE) model to investigate the transmission path of local government debt risk under real estate regulation. In addition, we analyze the risk transmission between the real estate market and local government under different tax systems and investigate the shock to household welfare from a local government debt default. The results show monetary policy can effectively control the scale of local government debt to reduce default risk. An increase in property taxes that restrains housing demand can effectively regulate the real estate market. Although reducing taxes can increase macroeconomic output, reducing tax rates on consumption, capital, and labor weakens the liquidity of household assets. Further, lowering taxes increases local government default risk, which reduces household welfare and makes it more difficult for local governments to deleverage. Our findings provide important insights for countries seeking an effective real estate regulation mechanism to curb local government default risk.
How does education promote green digital finance? Evidence from China
Authors: Lee Chien-Chiang , Wang Fuhao , Lee Chi-Chuan 
Published: January 2025

AbstractThis study constructs a new composite green digital finance index and describes its development in China over the past decade. Based on province-level panel data covering the period 2011–2020, we examine how education contributes to the development of green digital finance. Through detailed theoretical analysis and rigorous empirical testing, the following results are obtained. First, education directly promotes green digital finance by adjusting people’s consciousness and enhancing their abilities. Second, high-quality educational inputs, a high level of economic development, and a high degree of opening-up are among the important preconditions for education to further promote green digital finance in China. Third, education indirectly promotes green digital finance by driving industrial upgrades, increasing income levels, promoting technological innovation, and cultivating finance-related talent. This study offers policy recommendations to help improve education levels and suggests a valuable direction for further research on green digital finance.
Social support and aging: psychometric analysis of the ENRICHD Social Support Instrument in a Chilean population over 50
Authors: Salinas-Rehbein Belén , Terán-Mendoza Oscar , Cancino Vicente 
Published: January 2025

Abstract
Background
Social support is relevant to studying well-being, quality of life, and health during aging, particularly in people over 50. Therefore, brief instruments that allow its measurement within the clinical evaluation and research processes are necessary. The ENRICH Social Support Scale (ESSI) is a brief and easy-to-use instrument that measures the perception of social support; however, its psychometric properties in people over 50 in the Chilean context have yet to be tested.

Method
This study had a non-experimental, longitudinal panel design in which a sample of 303 people over 50 years of age (M = 55.14 years, SD = 5.19; 52% women) were administered a survey incorporating sociodemographic variables, the ESSI and the Newsom Social Negativity Scale. 277 participants completed the follow-up survey (M = 56.75 years, SD = 5.15; 54% women). Confirmatory factor analysis (CFA), structural equation modeling (SEM), invariance analysis, and internal consistency tests were performed to determine the psychometric properties.

Results
The six items from the scale load in a single-factor model obtained an excellent fit to the data and reliability coefficients (α = 0.902; ω = 0.904). The SEM analysis evidenced an inverse correlation between the ESSI and Newsom's social negativity scale, demonstrating evidence of construct validity. Furthermore, in the sex invariance analyses, the scalar level was reached, implying that the items’ meanings are the same for men and women. Finally, the residual level was reached in the temporal invariance analyses, which means that the scale items are consistent in time.

Conclusions
The ESSI is a reliable and valid tool to be used in the Chilean context both in the clinical setting and in research on people over 50. The evidence obtained supports its usability to appropriately measure the perception of social support, which is relevant considering that it has been associated with reports of higher quality of life and lower mortality and morbidity during aging.
Navigating Sense of Place through lived spaces and the memory of place in historic Anarkali Bazaar, Lahore
Authors: Batool Zunaira , Bahauddin Azizi 
Published: January 2025

AbstractHistoric fabric are increasingly at risk because they disregard the sense of place in urban regeneration projects, which jeopardises regional identities and customs. This study explores the processes underlying the creation of place meanings among users in historic urban bazaars, examining the attributes of lived space that contribute to the development of a sense of place and generate meaning through behavioural practices. Using the lived space of spatial triad theory as a theoretical framework, this research employs a hybrid methodological approach that combines phenomenological and grounded theory methods. Data are collected through semistructured interviews, onsite observations, and questionnaires, with analysis conducted via N-Vivo. By employing the lived space of spatial triad theory, this research offers a nuanced perspective on how historic urban bazaars function as living museums that foster pluralistic societies, cultural innovation, and economic fluidity within a contemplative environment. These insights underscore the importance of incorporating local memories and feelings into heritage conservation and urban regeneration projects, as these elements are crucial for maintaining the unique identity and vibrancy of historic sites. This study contributes to international scholarship on the lived space of historical sites by providing a focused examination of the dynamic forces shaping sense of place identity over time in historic bazaars.