bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental diagnosis of water quality in situ and ex situ in streams in Upper Paraná River Basin, Brazil
Authors: Meira Vandressa Raquel Lucas Poloni , Silva Elicléia Maria de Souza , Viana Lucilene Finoto , Crispim Bruno do Amaral , Francisco Luiza Flávia Veiga , Súarez Yzel Rondon , Barufatti Alexeia , Solórzano Júlio César Jut , Berti Alessandra Paim 
Published: June 2021

Abstract
The aim of this study was to (i) evaluate water quality using limnological parameters in three streams belonging to the Ivinhema River basin, (ii) investigate the composition and structure of the landscape around the locations sampled and their possible contribution to changes in water quality, (iii) investigate genotoxicity in fish in situ as environmental bioindicators, and (iv) evaluate the cytotoxic and genotoxic potential of water through bioassays in animal and plant models. A total of seven fish species were sampled in situ as bioindicators of environmental toxicogenetic damage: Astyanax fasciatus, Astyanax lacustris, Cyphocharax modestus, Moenkhausia forestii, Piabina argentea, Psellogrammus kennedyi, and Hypostomus ancistroides. Four types of nuclear and one cytoplasmic alteration were observed, with nuclear invagination being the most frequent. Animal bioassays showed five types of nuclear and one cytoplasmic alteration. In the plant bioassay, a reduction in germination and cell division was observed, indicating the cytotoxic and genotoxic effects of the water for Allium cepa cells. Thus, genotoxic alterations in fish and plant bioassays may reflect the different types of land use and cover around streams and the conversion of forest fragments into agricultural areas, mainly due to the expansion of sugarcane crops. The evaluation of the toxicogenetic status of the three streams is essential to emphasize the need to create projects to recover the native vegetation cover, which would improve the quality of the habitat and ensure the permanence of native species.
Exosome-mediated transfer of microRNAs promotes mouse iPSCs differentiation into therapeutic insulin-producing cells
Authors: Guo Qingsong , Lu Yuhua , Huang Yan , Guo Yibing , Zhu Shajun , Zhang Qiuqiang , Zhu Donghui , Wang Zhiwei , Luo Jia 
Published: June 2021

Abstract
Purpose

Exosome-based therapeutic approaches have been applied in diabetes. In the present study, we explored the effect of exosomes on iPSCs differentiation into insulin-producing cells and its underlying mechanisms.
Methods

Exosomes were isolated by ultracentrifugation from MIN6 cells and identified by Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blot. PKH67 tracer and transwell assay were used to confirm exosome delivery into iPSCs. QRT-PCR was applied to detect key pancreatic gene expression and miRNAs expression in differentiated iPSCs. Insulin expression was assessed by flow cytometry (FCM) and immunofluorescence. The mechanism underlying exosome induction capacity for iPSCs was determined via RNA-interference of Argonaute-2 (Ago2). Streptozotozin(STZ) was used to establish diabetic mouse model to verify the function of differentiated β-like cells.
Results

MIN6-derived exosomes promoted the key pancreatic gene expression and immunofluorescence for Nkx6.1 and insulin remarkably, confirming the capability of exosomes for iPSCs differentiation. Moreover, transplantation of differentiated iPSCs efficiently enhanced IPGTT and partially control hyperglycemia in T1D mice. Knockdown of Ago2 in MIN6 cells affect exosomal miRNAs expression and pancreatic gene expression and insulin secretion in iPSCs.The therapeutic effect in vivo was weakened, further indicating decreased exosomal miRNA affect iPSCs differentiation.7 specific exosomal miRNAs were selected for single-assay validation. MiR-706, miR-709, miR-466c-5p and miR-423-5p were found dynamic changed during differentiation stages.
Conclusion

Exosomes is an effective and convenient induction approach for iPSCs differentiation into functional insulin secreting cells.The effect was downregulated via Ago2 knockdown illustrates the mechanisms are highly relevant to specific miRNAs enriched in exosomes.
Domestic Violence in Married Couples: From the Viewpoint of Women
Authors: Areshtanab Hossein Namdar , Vaseai Fariba , Ebrahimi Hossein , Bostanabad Mohammad Arshadi , Hosseinzadeh Mina 
Published: June 2021

Abstract
Background: Domestic violence is one of the most common problems of public health that can be occurred in families and can lead to physical, psychological, and economic consequences individually, family, and social level. The present study was aimed to determine domestic violence of married couples from the Viewpoint of women. Methods: In this descriptive cross-sectional study 547 women referring to health centers of Marand in 2018 were participated. Sampling method was the convenience sampling method. For data collection, Socio-demographic and the Revised Conflict Tactics Scale (CTS2) questionnaires were used. Descriptive (mean, standard deviation, frequency, and percentage of frequency) and inferential statistics, including t-test were used to analyze the data. Results: Results showed that domestic violence against men (98.3%) and women (98.5%) has a high prevalence. Women have experienced more violence in the psychological, physical, and sexual dimensions and men have experienced more violence in the negotiation and injury dimensions. Conclusions: The most important message of study beyond the comparison of the numbers and the operative or the victim of violence is the insecurity of the family environment for women, men, and children, which can have serious consequences for the family and society in the future. According to the importance of family as one of the essential elements of a healthy society, a preventative proceeding is required.
Bleeding Complication in a Patient with Concomitant Use of Rivaroxaban and Saffron Supplement: A Case Report
Authors: Daei Maryam , Khalili Hossein , Heidari Zinat 
Published: June 2021

Abstract
Background

Direct oral anticoagulants (DOACs) carry a lower potential risk of food/herb and drug interactions compared with oral vitamin K antagonists. However, as a new class of medications some of these interactions have not been fully known.
Case presentation:

A 64-year old male with a medical history of non-valvular atrial fibrillation presented to the emergency department with a complaint of acute onset epistaxis and bleeding gums following the concomitant use of rivaroxaban and saffron supplement. Rivaroxaban plasma concentration was 54 ng/ml with a post-intake delay of 17 hours. The results of laboratory tests were unremarkable except for platelet function tests. Whole blood multiple electrode aggregometry was performed to assess platelet function. Area under the aggregation curve (AUC) values were 83 and 51 aggregation unit (AU)*min by arachidonic acid and adenosine diphosphate-induced platelet aggregation tests, respectively. As the patient had not taken any antiplatelet medication, platelet dysfunction was greatly attributed to the saffron supplement. The patient was immediately admitted to hospital and received local hemostatic measures and tranexamic acid. Moreover, saffron was discontinued permanently and rivaroxaban was paused for 24 hours. The bleeding stopped a few hours later and the patient was discharged after 2 days in a good general condition. Subsequently, he was followed up at 4, 8, and 12-week intervals. He was in a stable clinical condition with no bleeding complications. The patient was advised to consult with his doctor or pharmacist before taking any supplement or herbal medicine to ensure possible interactions.
Conclusions

It seems that coadministration of DOACs and saffron supplements should be avoided due to the potential drug-herbal interactions and possible risk of subsequent bleeding complications. However, further studies are needed to confirm the findings and assess the clinical significance.
H3K27 Acetylation of SF1 in PBMC: A Biomarker for Adrenal Insufficiency of Steroid Synthesis in Male Offspring Induced by Prenatal Dexamethasone Exposure
Authors: Chen Guanghui , Ai Can , Duan Fangfang , Chen Yawen , Cao Jiangang , Zhang Jinzhi , Ao Ying , Wang Hui 
Published: June 2021

Abstract
Dexamethasone is widely used for treating pregnant diseases. However, lots of studies have confirmed that prenatal dexamethasone exposure (PDE) could increase the risk of offspring multiple diseases. The purpose of this study was to elucidate the intrauterine epigenetic mechanism of adrenal developmental programming, and to explore early warning marker in peripheral blood mononuclear cells (PBMC). We found the adrenal morphological and functional changes before and after birth in PDE male offspring rats, which were performed as the decreases of serum corticosterone concentration, steroidogenic acute regulatory (StAR) expression, histone 3 lysine 27 acetylation (H3K27ac) level of steroidogenic factor1 (SF1) promoter region and its expression; meanwhile, the expression of glucocorticoid receptor (GR) and histone acetylation enzyme 5 (HDAC5) in PDE male fetal rats were increased. In vitro, dexamethasone reduced the expression of SF1 and StAR, and cortisol production, but increased the expression of GR and HDAC5, the binding between GR and SF1 promoter region, and protein interaction between GR and HDAC5. GR siRNA or HDAC5 siRNA could reverse the above effects of dexamethasone. Furthermore, in vivo, we confirmed that H3K27ac level of SF1 promoter region and its expression in PBMC of PDE group were decreased before and after birth, showing a positive correlation with the same indexes in adrenal. Meanwhile, in clinical trials, we confirmed that H3K27ac level of SF1 promoter region and its expression were decreased after prenatal dexamethasone application in neonatal PBMC. In conclusion, PDE induced adrenal insufficiency of male offspring rats, which was related to activate adrenal GR by dexamethasone in uterus. The activated GR, on one hand, increased its direct binding to SF1 promoter region to inhibit its expression, on the other hand, up-regulated and recruited HDAC5 to decrease H3K27ac level of SF1 promoter region, and strengthened the inhibition of SF1 and subsequent StAR expression.
The Effectiveness of Commercial Household Ultraviolet C Germicidal Devices in Thailand
Authors: Palakornkitti Pasita , Pinyowiwat Prinpat , Tanrattanakorn Somsak , Rajatanavin Natta , Rattanakaemakorn Ploysyne 
Published: June 2021

Abstract
Ultraviolet C (UVC), also called ultraviolet germicidal irradiation (UVGI), is known for its effective air, water, and surface disinfectant properties. With the rise of global awareness about public sanitation and personal hygiene due to the emergence of the current coronavirus disease 2019 pandemic, several applications of UVC were developed and introduced to the commercial market. The present experimental study was conducted to evaluate the effectiveness of commercial household ultraviolet C germicidal devices for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inactivation. UVC dosages (mJ/cm2) of 10 UVC devices were determined at the recommended settings and compared to a UVC dosage benchmark for SARS-CoV-2 inactivated UVC dosage (3.7 mJ/cm2). Of the 10 devices, 3 were handheld UVGI surface disinfection equipment, 4 were UVGI disinfection chambers, and 3 were movable UVGI air and surface purifiers. Three UVGI disinfection chambers and all movable UVGI air and surface purifiers provided sufficient UVC dosages for SARS-CoV-2 inactivation. None of the studied handheld UVGI surface disinfection equipment achieved the UVC dosage for SARS-CoV-2 inactivation. A lack of standardization in the distance and cycle duration for each UVC application was observed. Standard usage guidelines for UVC devices are required to improve the effectiveness of UVC irradiance for SARS-CoV-2 inactivation as well as to minimize the potential side effects of UVC.
Winter And Spring Atmospheric Rivers In High Mountain Asia: Climatology, Dynamics, And Variability
Authors: Nash Deanna , Carvalho Leila M.V. , Jones Charles , Ding Qinghua 
Published: June 2021

Abstract
Atmospheric Rivers (ARs) that reach the complex terrain of High Mountain Asia (HMA) cause significant hydrological impacts for millions of people. While ARs are often associated with precipitation extremes and can cause floods and debris flows affecting populated communities, little is known about ARs that reach as far inland as HMA. This paper characterizes AR types and investigates dynamical mechanisms associated with the development of ARs that typically affect HMA. Combined empirical orthogonal function (cEOF) analysis using integrated water vapor transport (IVT) is applied to days where an AR reaches HMA. K-means cluster analysis applied to the first two principal components uncovered three subtypes of AR events with distinct synoptic characteristics during winter and spring months. The first subtype increases precipitation and IVT in the Karakoram and is associated with a zonally oriented wave train propagating within the westerly jet waveguide. The second subtype is associated with enhanced southwesterly IVT, anomalous upper-level cyclonic circulation centered on 45°E, and precipitation in the Pamirs. The third subtype shows anomalous precipitation in the Eastern Himalayas and southwesterly IVT across the Bay of Bengal. Interannual variations in the frequency of HMA ARs and relationships with various teleconnection patterns show that western HMA AR subtypes are sensitive to well-known remote large-scale climate factors, such as the El Niño Southern Oscillation, Arctic Oscillation, and the Siberian High. These results provide synoptic characterization of the three types of ARs that reach HMA and reveal the previously unexplored significance of their contribution to winter and spring precipitation.
Alteration of the Optical and Mechanical Characteristics of Cellulose Nanofibre Films Under Thermal Treatment
Authors: Niskanen Ilpo , Zhang Kaitao , Liimatainen Henrikki , Shibata Shuhei , Hagen Nathan , Heikkilä Rauno , Yoda Hidehiko , Otani Yukitoshi 
Published: June 2021

Abstract
Nanocelluloses and their different designs, such as films and nanopapers, have gained considerable interest in many application areas due to their unique properties. For many purposes, such as for packaging and electronics, the thermal stability of nanocellulose materials is a crucial characteristic. In this study, the effects of heat treatment (100ºC, 150ºC and 200ºC) on the optical and mechanical properties of 2,2,6,6-tetramethylpiperidinyl-1-oxy radical-oxidised cellulose nanofibre (TO-CNF) films were investigated, especially the alteration of the colour, complex refractive index and birefringence of the films. Exposing TO-CNF films to high temperatures (> 150ºC) induced permanent transformations in the CNF structure, leading to an increase in the refractive index, decreases in the birefringence and crystallinity index, colour darkening and significant deterioration of the mechanical properties.
iTRAQ-Based Quantitative Proteome Analysis Insights into Cold Stress of Winter Turnip Rapa (Brassica rapa L.) Grown in the Field
Authors: Niu Zaoxia , Liu Lijun , Pu Yuanyuan , Ma Li , Wu Junyan , Hu Fangdi , Fang Yan , Li Xuecai , Sun Wancang , Bai Chunsheng , Wang Wangtian 
Published: June 2021

Abstract
Winter Turnip rapa (Brassica rapa L.) is a major oilseed crop in Northern China, where its production was severely affected by chilling and freezing stress. Previous studies have demonstrated that differentially accumulated proteins (DAPs) were expressed in roots and leaves under control chilling stress. In this study, the isobaric tag for relative and absolute quantification (iTRAQ) technology was performed to identify DAPs under freezing stress. Two winter rapaseed varieties, Longyou 7 (cold-tolerant) and Lenox (cold-sensitive), were used to investigated morphological, physiological, cell and protein levels in the shoot apical meristem (SAM) of field-grown Brassica rapa to reveal the molecular mechanisms of cold stress tolerance. Compared to Lenox, Longyou 7 had a lower SAM of height, higher collar diameter. The level of malondialdehyde (MDA), SAM of height and IAA content were repressed. At the same time, compared to Lenox, the soluble sugars (SS) content, superoxide dismutase (SOD) activity, peroxidase(POD)activity, soluble protein (SP) content and collar diameter increased in Longyou 7. In total, we identified 6330 proteins, among this, 98 DAPs were expressed in L7 CK/Le CK, 107 DAPs were expressed in L7 d /Le d 183 DAPs were expressed in Le d /Le CK, 111 DAPs were expressed in L7 d /L7 CK. Quantitative real-time PCR (RT-qPCR) analysis of the coding genes for seventeen randomly selected DAPs were performed for validation. These DAPs were identified from the two winter rapa seed cultivars involved in the biological process, cellular component and molecular function analysis, which revealed glutathione transferase activity, carbohydrate-binding, and glutathione binding, glutathione metabolic process and response IAA were closely associated with the cold stress response. Some cold-induced proteins, such as glutathione S-transferase phi 2(GSTF2), might play essential roles cold acclimation in Brassica rapa of SAM. Our work will help to provide valuable information for responding to the cold stress in Brassica rapa L.
Gasdermin E-dependent and -independent Subroutines Regulate the Passage of Dextrans During Apoptosis-driven Secondary Necrosis.
Authors: De Schutter Elke , Ramon Jana , Pfeuty Benjamin , De Tender Caroline , Stremersch Stephan , Raemdonck Koen , de Beeck Ken Op , Declercq Wim , Riquet Franck B. , Braeckmans Kevin , Vandenabeele Peter 
Published: June 2021

Abstract
Secondary necrosis has long been perceived as an uncontrolled process resulting in total lysis of the apoptotic cell. Recently, it was shown that progression of apoptosis to secondary necrosis is regulated by Gasdermin E (GSDME), which requires activation by caspase-3. Although the contribution of GSDME in this context has been attributed to its pore-forming capacity, little is known about the kinetics and size characteristics of this. Here we report on the membrane permeabilizing features of GSDME by monitoring the influx and efflux of dextrans of different sizes into/from anti-Fas stimulated L929sAhFas cells undergoing apoptosis-driven secondary necrosis. We found that GSDME accelerates cell lysis measured by SYTOX Blue staining but does not affect the exposure of phosphatidylserine on the plasma membrane. Furthermore, loss of GSDME expression clearly hampered the influx of fluorescently labeled dextrans while the efflux happened independent of the presence or absence of GSDME expression. Importantly, both in- and efflux of dextrans was dependent on their molecular weight. Altogether, our results demonstrate that GSDME regulates the passage of compounds together with other plasma membrane destabilizing subroutines.
Shotgun-Metagenomics Based Prediction Of Antibiotic Resistance And Virulence Determinants In Staphylococcus Aureus From Prosthetic Joint Tissue On Blood Culture Bottles
Authors: Sanabria Adriana Maria , Janice Jessin , Hjerde Erik , Simonsen Gunnar Skov , Hanssen Anne-Merethe 
Published: June 2021

Abstract
Shotgun-metagenomics may give valuable clinical information beyond the detection of potential pathogen(s). Identification of antimicrobial resistance (AMR), virulence genes and typing directly from clinical samples has been limited due to challenges arising from incomplete genome coverage. We assessed the performance of shotgun-metagenomics on positive blood culture bottles (n = 19) with prosthetic joint tissue for typing and prediction of AMR and virulence profiles in Staphylococcus aureus. We used different approaches to determine if sequence data from reads provides more information than from assembled contigs. Only 0.18% of total reads was derived from human DNA. Shotgun-metagenomics results and conventional method results were consistent in detecting S. aureus in all samples. AMR and known prosthetic joint infection virulence genes were predicted from S. aureus. Mean coverage depth, when predicting AMR genes was 209x. Resistance phenotypes could be explained by genes predicted in the sample in most of the cases. The choice of bioinformatic data analysis had a significant impact on the results. Read-based analysis was more accurate for pathogen identification, while contigs seemed better for AMR profiling. Our study demonstrates high genome coverage and potential for typing and prediction of AMR and virulence profiles in S. aureus from shotgun-metagenomics data.
An Optimal Window of Platelet Reactivity by LTA Assay for Patients Undergoing Percutaneous Coronary Intervention
Authors: Wang Jing , Ma Jiazheng , Teng Jianzhen , Zhang Xiaofeng , Wang Jing , Gu Qian , Ye Zekang , Ullah Inam , Tan Chuchu , Abdus Samee , Shi Lu , Gong Xiaoxuan , Li Chunjian 
Published: June 2021

Abstract
Objective This study was aimed to investigate an optimal therapeutic window for platelet reactivity (PR) to predict the lowest ischemic and bleeding events in patients underwent percutaneous coronary intervention (PCI) and treated with dual antiplatelet agents. Design A total of 1709 patients who had received coronary stent implantation and had taken aspirin 100 mg in combination with clopidogrel 75 mg daily for >5 days were consecutively recruited and their platelet reactivity was determined by light transmittance aggregometry (LTA). All patients were followed up for 12 months. The primary end-point was the net adverse clinical events (NACE) of cardiovascular death, nonfatal myocardial infarction (MI), target vessel revascularization (TVR) , stent thrombosis (ST) and any bleeding.Result By using the receiver-operating curve (ROC) analysis, the optimal cutoff values were found to be 37.5% and 25.5% respectively in predicting ischemic and bleeding events. Patients were classified into 2 groups according to PR: inside the window group (IW) [adenosine diphosphate (ADP) induced platelet aggregation (PLADP) 25.5%-37.4%)] and outside the window group (OW) (PLADP <25.5% or ≥37.5%). The incidence of NACE was 16.8% and 23.1% respectively in the IW and OW group. The hazard ratio of NACE in IW-group was significantly lower [0.69 (95% CI: 0.54–0.89; P = 0.004)] than that in the OW-group during 12 month follow-up. Conclusion An optimal therapeutic window of 25.5%-37.4% for PLADP predicts the lowest risk of NACE, which could be referred for tailored antiplatelet treatment while using LTA assay. Trial and clinical registry Trial registration number: ClinicalTrials.gov NCT01968499. Registered 18 October 2013 - Retrospectively registered.