bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ring finger protein 213 c.14576G>A mutation is not involved in internal carotid artery and middle cerebral artery dysplasia
Authors: Murai Yasuo , Ishisaka Eitaro , Watanabe Atsushi , Sekine Tetsuro , Shirokane Kazutaka , Matano Fumihiro , Nakae Ryuta , Tamaki Tomonori , Koketsu Kenta , Morita Akio 
Published: November 2021

AbstractThe ring finger protein 213 (RNF213) susceptibility gene has been detected in more than 80% of Japanese and Korean patients with moyamoya disease (MMD), a bilateral internal carotid artery (ICA) occlusion. Furthermore, RNF213 has been detected in more than 20% of East Asians with atherosclerotic ICA stenosis. In this study, we evaluated the frequency of RNF213 mutations in congenital occlusive lesions of the ICA system. This case series was conducted jointly at four university hospitals. Patients with a family history of MMD, quasi-MMD, or related diseases were excluded. Ten patients were diagnosed with abnormal ICA or middle cerebral artery (MCA) angiogenesis. Patients with neurofibromatosis were excluded. Finally, nine patients with congenital vascular abnormalities were selected; of these, five had ICA deficiency and four had twig-like MCA. The RNF213 c.14576G > A mutation was absent in all patients. Therefore, the RNF213 c.14576G > A mutation may not be associated with ICA and MCA congenital dysplasia—rare vascular anomalies making it difficult to study a large number of cases. However, an accumulation of cases is required for accurate determination. The results of this study may help differentiate congenital vascular diseases from MMD.
Preparation and characteristic analysis of nanofacula array
Authors: Shao Lina , Tian Xin , Ji Shengxiang , Wang Hongda , Shi Yan 
Published: November 2021

AbstractThe development of nanofacula array is an effective methods to improve the performance of Near-field Scanning Optical Microscopy (NSOM) and achieve high-throughput array scanning. The nanofacula array is realized by preparing metal nanopore array through the "two etching-one development" method of double-layer resists and the negative lift-off process after metal film coating. The shading property of metal film plays important rules in nanofacula array fabrication. We investigate the shading coefficient of three kinds of metal films (gold–palladium alloy (Au/Pd), platinum (Pt), chromium (Cr)) under different coating times, and 3.5 min Au/Pd film is determined as the candidate of the nanofacula array fabrication for its lower thickness (about 23 nm) and higher shading coefficient (≥ 90%). The nanofacula array is obtained by irradiating with white light (central wavelength of 500 nm) through the metal nanopore array (250/450 nm pore diameter, 2 μm pore spacing and 7 μm group spacing). Moreover, the finite difference and time domain (FDTD) simulation proves that the combination of nanopore array and microlens array achieves high-energy focused nanofacula array, which shows a 3.2 times enhancement of electric field. It provides a new idea for NSOM to realize fast super-resolution focusing facula array.
Correlation between brain function and ADHD symptom changes in children with ADHD following a few-foods diet: an open-label intervention trial
Authors: Hontelez Saartje , Stobernack Tim , Pelsser Lidy M. , van Baarlen Peter , Frankena Klaas , Groefsema Martine M. , Kleerebezem Michiel , Rodrigues Pereira Rob , Postma Elbrich M. , Smeets Paul A. M. , Stopyra Marion A. , Zwiers Marcel P. , Aarts Esther 
Published: November 2021

AbstractResearch into the effect of nutrition on attention-deficit hyperactivity disorder (ADHD) in children has shown that the few-foods diet (FFD) substantially decreases ADHD symptoms in 60% of children. However, the underlying mechanism is unknown. In this open-label nutritional intervention study we investigated whether behavioural changes after following an FFD are associated with changes in brain function during inhibitory control in 79 boys with ADHD, aged 8–10 years. Parents completed the ADHD Rating Scale before (t1) and after the FFD (t2). Functional magnetic resonance imaging (fMRI) scans were acquired during a stop-signal task at t1 and t2, and initial subject-level analyses were done blinded for ARS scores. Fifty (63%) participants were diet responders, showing a decrease of ADHD symptoms of at least 40%. Fifty-three children had fMRI scans of sufficient quality for further analysis. Region-of-interest analyses demonstrated that brain activation in regions implicated in the stop-signal task was not associated with ADHD symptom change. However, whole-brain analyses revealed a correlation between ADHD symptom decrease and increased precuneus activation (pFWE(cluster) = 0.015 for StopSuccess > Go trials and pFWE(cluster) < 0.001 for StopSuccess > StopFail trials). These results provide evidence for a neurocognitive mechanism underlying the efficacy of a few-foods diet in children with ADHD.
A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines
Authors: Eggenschwiler Reto , Gschwendtberger Thomas , Felski Christian , Jahn Christopher , Langer Florian , Sterneckert Jared , Hermann Andreas , Lühmann Jonathan , Steinemann Doris , Haase Alexandra , Martin Ulrich , Petri Susanne , Cantz Tobias 
Published: November 2021

AbstractCRISPR prime-editors are emergent tools for genome editing and offer a versatile alternative approach to HDR-based genome engineering or DNA base-editors. However, sufficient prime-editor expression levels and availability of optimized transfection protocols may affect editing efficiencies, especially in hard-to-transfect cells like hiPSC. Here, we show that piggyBac prime-editing (PB-PE) allows for sustained expression of prime-editors. We demonstrate proof-of-concept for PB-PE in a newly designed lentiviral traffic light reporter, which allows for estimation of gene correction and defective editing resulting in indels, based on expression of two different fluorophores. PB-PE can prime-edit more than 50% of hiPSC cells after antibiotic selection. We also show that improper design of pegRNA cannot simply be overcome by extended expression, but PB-PE allows for estimation of effectiveness of selected pegRNAs after few days of cultivation time. Finally, we implemented PB-PE for efficient editing of an amyotrophic lateral sclerosis-associated mutation in the SOD1-gene of patient-derived hiPSC. Progress of genome editing can be monitored by Sanger-sequencing, whereas PB-PE vectors can be removed after editing and excised cells can be enriched by fialuridine selection. Together, we present an efficient prime-editing toolbox, which can be robustly used in a variety of cell lines even when non-optimized transfection-protocols are applied.
Penetrative and non-penetrative interaction between Laboulbeniales fungi and their arthropod hosts
Authors: Reboleira Ana Sofia P. S. , Moritz Leif , Santamaria Sergi , Enghoff Henrik 
Published: November 2021

AbstractLaboulbeniales are a highly specialized group of fungi living only on arthropods. They have high host specificity and spend their entire life-cycle on an arthropod host. Taxonomic characters of Laboulbeniales are based on the architecture of the cells of the parenchymal thallus, i.e. the visible part of the fungus outside the host. The extent of the fungus spreading inside the host—the haustorium—remains largely unknown. The attachment to the arthropod host is fundamental to understand the fungus-animal interaction, but how this truly occurs is unclear. Recent evidences question the strictly parasitic life-style of Laboulbeniales. We used micro-computed tomography (µCT) and 3D reconstructions to visualize, for the first time, the complete structure of Laboulbeniales species in situ on their hosts. We compared the haustoriate species, Arthrorhynchus nycteribiae on an insect host to the non-haustoriate species, Rickia gigas on a millipede host. Our results confirm that some Laboulbeniales species are ectoparasitic and have a haustorial structure that penetrates the host’s cuticle, while others are ectobionts and are only firmly attached to the host’s cuticle without penetrating it. The presence and the morphology of the haustorium are important traits for Laboulbeniales evolution, and key factors for future understanding of host dependence and specificity.
Impact of ceftolozane/tazobactam concentrations in continuous infusion against extensively drug-resistant Pseudomonas aeruginosa isolates in a hollow-fiber infection model
Authors: Montero María M. , Domene-Ochoa Sandra , López-Causapé Carla , Luque Sonia , Sorlí Luisa , Campillo Núria , Padilla Eduardo , Prim Núria , Ferrer-Alapont Lorena , Angulo-Brunet Ariadna , Grau Santiago , Oliver Antonio , Horcajada Juan P. 
Published: November 2021

AbstractCeftolozane/tazobactam (C/T) has emerged as a potential agent for the treatment of extensively drug-resistant (XDR) Pseudomonas aeruginosa infections. As it is a time-dependent antimicrobial, prolonged infusion may help achieve pharmacokinetic/pharmacodynamic (PK/PD) targets. To compare alternative steady-state concentrations (Css) of C/T in continuous infusion (CI) against three XDR P. aeruginosa ST175 isolates with C/T minimum inhibitory concentration (MIC) values of 2 to 16 mg/L in a hollow-fiber infection model (HFIM). Duplicate 10-day HFIM assays were performed to evaluate Css of C/T in CI: one compared 20 and 45 mg/L against the C/T-susceptible isolate while the other compared 45 and 80 mg/L against the two C/T-non-susceptible isolates. C/T resistance emerged when C/T-susceptible isolate was treated with C/T in CI at a Css of 20 mg/L; which showed a deletion in the gene encoding AmpC β-lactamase. The higher dosing regimen (80 mg/L) showed a slight advantage in effectiveness. The higher dosing regimen has the greatest bactericidal effect, regardless of C/T MIC. Exposure to the suboptimal Css of 20 mg/L led to the emergence of C/T resistance in the susceptible isolate. Antimicrobial regimens should be optimized through C/T levels monitoring and dose adjustments to improve clinical management.
Soil bacterial community as impacted by addition of rice straw and biochar
Authors: Tang Zhiqiang , Zhang Liying , He Na , Gong Diankai , Gao Hong , Ma Zuobin , Fu Liang , Zhao Mingzhu , Wang Hui , Wang Changhua , Zheng Wenjing , Zhang Wenzhong 
Published: November 2021

AbstractThe application of straw and biochar can effectively improve soil quality, but whether such application impacts paddy soil bacterial community development remains to be clarified. Herein, the impacts of three different field amendment strategies were assessed including control (CK) treatment, rice straw (RS) application (9000 kg ha−1), and biochar (BC) application (3150 kg ha−1). Soil samples were collected at five different stages of rice growth, and the bacterial communities therein were characterized via high-throughput 16S rDNA sequencing. The results of these analyses revealed that soil bacterial communities were dominated by three microbial groups (Chloroflexi, Proteobacteria and Acidobacteria). Compared with the CK samples, Chloroflexi, Actinobacteria, Nitrospirae and Gemmatimonadetes levels were dominated phyla in the RS treatment, and Acidobacteria, Actinobacteria, Nitrospirae and Patescibacteria were dominated phyla in the BC treatment. Compared with the RS samples, Chloroflexi, Acidobacteria, Actinobacteria, and Verrucomicrobia levels were increased, however, Proteobacteria, Gemmatimonadetes, Nitrospirae, and Firmicute levels were decreased in the BC samples. Rhizosphere soil bacterial diversity rose significantly following RS and BC amendment, and principal component analyses confirmed that there were significant differences in soil bacterial community composition among treatment groups when comparing all stages of rice growth other than the ripening stage. Relative to the CK treatment, Gemmatimonadaceae, Sphingomonadaceae, Thiovulaceae, Burkholderiaceae, and Clostridiaceae-1 families were dominant following the RS application, while Thiovulaceae and uncultured-bacterium-o-C0119 were dominant following the BC application. These findings suggest that RS and BC application can improve microbial diversity and richness in paddy rice soil in Northeast China.
Anion-controlled Zn(ii) coordination polymers with 1-(tetrazo-5-yl)-3-(triazo-1-yl) benzene as an assembling ligand: synthesis, characterization, and efficient detection of tryptophan in water
Authors: Sun Han-Xu , Zhou Jie , Zhang Zhen , He Mei , He Lian-Cheng , Du Lin , Xie Ming-Jin , Zhao Qi-Hua 
Published: November 2021

The triazole/tetrazole N-donor ligand of Httb is rarely studied in the construction of CPs. A controlling effect of anions on supramolecular architectures has been observed. CP 2 has been proved to be the best tryptophan sensor.
Synthesis and characterization of 1-butyl-4-amino-1,2,4-triazolium energetic ionic liquids with transition-metal complex anions
Authors: Bo Gao , Zhanning Niu , Hongwei Yang , Haoqing Tang , Xiaohuan Liu , Tian Tian 
Published: November 2021

Energetic ionic liquids based on 1-butyl-4-amino-1,2,4-triazolium cation and transition metal complex anions, namely [Co(NO2)6]3−, [Co(CN)6]3−, [Co(NCS)4]2− and [Fe(CN)6]3−, were synthesized.
Contemplative Pedagogy: Creating Mindful Educators and Classrooms
Authors: Chapman Laura Roche 
Published: November 2021

Purpose:This tutorial introduces communication sciences and disorders (CSD) educators to contemplative pedagogy (sometimes called mindfulness in the classroom). While contemplative pedagogy has considerable overlap with evidence-based teaching and learning, and aligns with values in the CSD discipline, there are few published reports investigating the role of contemplative pedagogy in CSD education. This tutorial outlines the potential benefits of contemplative pedagogy to CSD education and offers suggestions on how to create mindful educators and classrooms. Specific contemplative practices are described. Basic instructions, along with ideas and examples of how practices can be modified and contextualized in CSD classrooms, are provided.Conclusions:Educators can bring contemplative practices into CSD classrooms in many ways. Educators can cultivate a contemplative disposition through a personal practice that can enhance the development of self-awareness. Self-awareness can then inform critical reflection of teaching. Educators can select and design contemplative practices that integrate students' first-, second-, and third-person ways of knowing. As an addition to the pedagogical toolbox, contemplative pedagogy offers opportunities for educators and students to connect their own values and experiences with the process of teaching and learning.
Predictive‐based optimal automatic formation control of mobile vehicles
Authors: Zanma Tadanao , Haga Shunta , Koiwa Kenta , Liu Kang‐Zhi 
Published: November 2021

AbstractIn recent years, formation control has received a great deal of attention as one of the most interesting issues in multiple mobile robots systems. In variable formation control systems, multiple mobile vehicles can form an appropriate formation from a list of possible formations, such that all mobile vehicles can pass even when the width of the course is narrow, and simultaneously the vehicles maintain a set distance between the course and each vehicle. This paper presents a predictive‐based automatic formation control. In our formation control system, a unique formation is determined from feasible formations at any given sampling point. This control is formulated by its corresponding mixed‐integer quadratic programing by introducing binary variables that are used to specify the formation. The effectiveness of the proposed methods is verified by applying two‐wheeled mobile vehicles through simulations and experiments.
Effect of graphene modified semi‐conductive shielding layer on space charge accumulation in insulation layer for high‐voltage direct current cable
Authors: Li Guochang , Li Xuejing , Zhang Fan , Liang Xiaojian , Liu Mingyue , Wei Yanhui , Li Shengtao , Hao Chuncheng , Lei Qingquan 
Published: November 2021

AbstractSpace charge accumulation in the insulation layer of high‐voltage direct current (HVDC) cable is one of the key factors restricting the development of HVDC cable. The inner semi‐conductive layer as an important structure of cable will affect the charge accumulation characteristics in the insulation layer. This work intends to explore the method of modifying the semi‐conductive layer with graphene, to suppress charge accumulation in the insulation layer. First, the semi‐conductive layer with different contents of Graphene (G) and carbon black (CB) are prepared. Second, the morphology and surface roughness of the cross section are analysed. Further, the effects of the semi‐conductive layer on space charge accumulation of the insulation layer are studied by pulsed electro‐acoustic method and thermal stimulation depolarisation current method. The experimental results show that a moderate amount of graphene replacing CB can effectively reduce charge accumulation in the insulation layer and inhibit positive temperature coefficient (PTC) effect at the same time. The surface roughness of specimens decreases with the increase of G content from 0 to 3 phr (parts per hundreds of resin), when the CB content decreases from 25 to 10 phr, the surface roughness of specimen increases. The resistivity test shows that doping G can significantly inhibit the PTC effect of the semi‐conductive layer. The volume resistivity of the semi‐conductive layer decreases with the increase of G content and CB content. In addition, charge accumulation of the insulation layer rises and then drops under the action of the semi‐conductive layer.
Hybridization of battery with pico hydel for frequency regulation of microgrids using synchronverter control
Authors: Vasudevan Krishnakumar R. , Ramachandaramurthy Vigna K. , Venugopal Gomathi , Guerrero Josep M. 
Published: November 2021

AbstractFrequency regulation of autonomous microgrids with intermittent renewables is a tedious task, which requires additional support from energy storage systems (ESS). Ideally, the system requires a faster‐acting ESS to arrest the initial frequency deviation and high energy capacity ESS to restore the frequency to its nominal value. Pico hydel energy storage (PHES) is one such high energy capacity ESS developed from the agricultural pumping systems. However, PHES has a slower response and cannot switch rapidly between pumping and generation modes. Hence, this paper incorporates a battery with PHES to achieve a faster dynamic response and absorb high‐frequency power dynamics. Additionally, a frequency regulation strategy with synchronverter control is proposed to mimic synchronous machines, especially to reduce the rate of change of frequency (ROCOF) and frequency nadir. Firstly, a small‐signal modelling approach is proposed, and it was utilized to assess the system stability through eigenvalue analysis. Subsequently, transient simulations were carried out to gauge the performance of the synchronverter strategy with vector control for frequency regulation. As a result, the frequency nadir and ROCOF were substantially reduced with the synchronverter strategy compared to the vector control based on the simulation results.
A review of short‐term wind power probabilistic forecasting and a taxonomy focused on input data
Authors: Bazionis Ioannis K. , Karafotis Panagiotis A. , Georgilakis Pavlos S. 
Published: November 2021

AbstractA review of state‐of‐the‐art short‐term wind power probabilistic forecasting models is the focus here. The improvement of the accuracy and efficiency of probabilistic forecasting models has been in the centre of attention of researchers in recent years, since the need to further comprehend and efficiently use the uncertainty of forecasts is increasing. Since the optimal operation and control of energy systems and electricity markets is one of the important aspects of performing wind power forecasts, this review focuses in short‐term probabilistic forecasting models, which could prove to be useful in the daily planning and operation of power systems. The short‐term concept of forecasts is analysed in detail, along with the case studies and examples proposed by the reviewed literature. The key advantages and disadvantages of the reviewed probabilistic forecasting methodologies are identified. Furthermore, different classifications of the reviewed works according to the data that are used to provide an accurate forecasting model are also provided. Future directions in the field of short‐term wind power probabilistic forecasting are also proposed.
Intrinsic polarization of Wolf-Rayet stars due to the rotational modulation of the stellar wind
Authors: Abdellaoui S. , Krtička J. , Kurfürst P. 
Published: November 2021

Context. Fast rotating Wolf-Rayet stars are expected to be progenitors of long duration gamma-ray bursts. However, the observational test of this model is problematic. Spectral lines of Wolf-Rayet stars originate in expanding stellar wind, therefore a reliable spectroscopical determination of their rotational velocities is difficult. Intrinsic polarization of Wolf-Rayet stars due to the rotational modulation of the stellar wind may provide an indirect way to determine the rotational velocities of these stars. However, detailed wind models are required for this purpose.
Aims. We determine the intrinsic polarization of Wolf-Rayet stars from hydrodynamical wind models as a function of rotational velocity.
Methods. We used 2.5D hydrodynamical simulations to calculate the structure of rotating winds of Wolf-Rayet stars. The simulations account for the deformation of the stellar surface due to rotation, gravity darkening, and nonradial forces. From the derived models, we calculated the intrinsic stellar polarization. The mass loss rate was scaled to take realistic wind densities of Wolf-Rayet stars into account.
Results. The hydrodynamical wind models predict a prolate wind structure, which leads to a relatively low level of polarization. Even relatively large rotational velocities are allowed by observational constrains. The obtained wind structure is similar to that obtained previously for rotating optically thin winds.
Conclusions. Derived upper limits of rotational velocities of studied Wolf-Rayet stars are not in conflict with the model of long duration gamma-ray bursts.