bluuebunny/crossref_metadata_2025_split huggingface dataset:
RBM5-AS1 promotes radioresistance in medulloblastoma through stabilization of SIRT6 protein
Authors: Zhu Chuanying , Li Keke , Jiang Mawei , Chen Siyu 
Published: July 2021

AbstractCancer stem cells (CSCs) contribute to radioresistance in medulloblastoma. Thus, identification of key regulators of medulloblastoma stemness is critical for improving radiotherapy for medulloblastoma. In the present study, we profiled CSC-related long non-coding RNAs (lncRNAs) between radioresistant and parental medulloblastoma cells. The roles of the lncRNA RBM5-AS1 in the stemness and radiosensitivity of medulloblastoma cells were investigated. We found that RBM5-AS1, a novel inducer of medulloblastoma stemness, was significantly upregulated in radioresistant medulloblastoma cells compared to parental cells. Knockdown of RBM5-AS1 diminished the viability and clonogenic survival of both radioresistant and parental medulloblastoma cells after radiation. Silencing of RBM5-AS1 significantly enhanced radiation-induced apoptosis and DNA damage. In vivo studies confirmed that depletion of RBM5-AS1 inhibited tumor growth and increased radiosensitivity in a medulloblastoma xenograft model. In contrast, overexpression of RBM5-AS1 reduced radiation-induced apoptosis and DNA damage in medulloblastoma cells. Mechanistically, RBM5-AS1 interacted with and stabilized sirtuin 6 (SIRT6) protein. Silencing of SIRT6 reduced the stemness and reinforced radiation-induced DNA damage in medulloblastoma cells. Overexpression of SIRT6 rescued medulloblastoma cells from RBM5-AS1 depletion-induced radiosensitization and DNA damage. Overall, we identify RBM5-AS1 as an inducer of stemness and radioresistance in medulloblastoma. Targeting RBM5-AS1 may represent a potential strategy to overcome the resistance to radiotherapy in this malignancy.
Strategies to improve pharmaceutical supply chain resilience under politico-economic sanctions: the case of Iran
Authors: Bastani Peivand , Dehghan Zahra , Kashfi Seyyed Mansoor , Dorosti Hesam , Mohammadpour Mohammadtaghi , Mehralian Gholamhossein , Ravangard Ramin 
Published: July 2021

AbstractBackgroundGiven the impact of politico-economic sanctions on the pharmaceutical supply chain, this study aims to identify practical strategies to improve the resilience of the Iranian supply chain in pharmaceutical procurement under politico-economic sanctions.MethodsThis is a qualitative content analysis study conducted in 2018. Semi-structured interviews were conducted using snowball sampling, and saturation was achieved after 18 interviews. Guba and Lincoln's criteria, namely credibility, confirmability, transferability, and dependability, were considered to ensure the validity and transparency of the study. A five-step framework analysis was applied to analyze the data using MAX QDA10.ResultsThe results led to the identification of nine main themes and 26 subthemes as strategies to improve the resilience of the pharmaceutical chain. According to the thematic map, some of these strategies have an extra-sectoral character: ‘insurance organizations’, ‘strengthening relations with other countries’, ‘mechanization of the distribution system’, and ‘suppliers and manufacturers’. At the same time, some inter-sectoral strategies can help the pharmaceutical chain maintain its resilience: ‘healthcare management and policy’, ‘exploiting local potential’, ‘pricing’, and ‘integrated health information systems.’ As a strategy, ‘Medical community and consumers’ also plays a crucial role in this regard. According to the subthemes, revisions of health management, more supervision, privatization, clinical policies, strategic purchasing, improvement of the referral system, inter-sectoral cooperation, support of indigenous medicines, rational pricing, insurance system, improvement of medical coverage, and development of electronic prescription should be considered by health systems. Sufficient support for indigenous medication and supervision of the distribution system should be considered by the pharmaceutical industry, taking into account the cooperation between consumers and patients.ConclusionsIntegration of the pharmaceutical supply chain and modern technologies, more attention to business complexity, economic development, intense competition, rapid changes in customer needs, and appropriate relationship between manufacturers, distributors, prescribers, and insurance organizations as purchasers should be considered by policymakers to improve supply chain resilience.
Dynamic earthquake sequence simulation with a SBIEM without periodic boundaries
Authors: Noda Hiroyuki 
Published: July 2021

AbstractDynamic earthquake sequence simulation is an important tool for investigating the behavior of a fault that hosts a series of earthquakes because it solves all interrelated stages in the earthquake cycle consistently, including nucleation, propagation and arrest of dynamic rupture, afterslip, locking, and interseismic stress accumulation. Numerically simulating and resolving these phenomena, which have different time and length scales, in a single framework is challenging. A spectral boundary integral equation method (SBIEM) that makes use of a fast Fourier transform is widely used because it reduces required computational costs, even though it can only be used for a planar fault. The conventional SBIEM has a periodic boundary condition as a result of the discretization of the wavenumber domain with a regular mesh; thus, to obtain an approximate solution for a fault in an infinite medium, it has been necessary to simulate a region much longer than the source distribution. Here, I propose a new SBIEM that is free from this artificial periodic boundary condition. In the proposed method, the periodic boundaries are removed by using a previously proposed method for the simulation of dynamic rupture. The integration kernel for the elastostatic effect, which reaches infinitely far from the source, is expressed analytically and replaces the one in the conventional SBIEM. The new method requires simulation of a region only twice as long as the source distribution, so the computational costs are significantly less than those required by the conventional SBIEM to simulate a fault in an infinite medium. The effect of the distance λ between the artificial periodic boundaries was investigated by comparing solutions for a typical problem setting between the conventional and proposed SBIEM. The result showed that the artificial periodic boundaries cause overestimation of the recurrence interval that is proportional to λ−2. If λ is four times the fault length, the interval is overestimated by less than 1%. Thus, the artificial periodic boundaries have only a modest effect on the conclusions of previous studies.
Nutritional status and quality of life in diabetic patients on hemodialysis: a cross-sectional study from Palestine
Authors: Hafi Eba’a , Soradi Ro’ya , Diab Sarah , Samara Ahmad M. , Shakhshir Marah , Alqub Malik , Zyoud Sa’ed H. 
Published: July 2021

Abstract
Background
End-stage renal disease (ESRD) is a leading cause of death and morbidity worldwide. Malnutrition is a common problem among hemodialysis (HD) patients that negatively impacts their prognosis and is linked to an increase in morbidity and mortality in these patients, as well as a decrease in their quality of life (QOL). In this study, we aimed to evaluate the QOL and to investigate factors that can influence it, including nutritional status, as well as socio-demographic factors, among Palestinian diabetic patients on HD therapy.

Methods
This was a cross-sectional study that occurred at a large hemodialysis center in Palestine. Malnutrition was assessed by the malnutrition-inflammation scale (MIS), and the quality of life was evaluated by using the EuroQoL five-dimensional instrument (EQ-5D). Multivariable linear regression analysis was carried out to look at the effect of multiple variables on QOL.

Results
A total of 118 diabetic patients on HD were included. Of these, 66.9% were male, and 60.2% were aged 60 years or higher. Having multiple comorbid diseases (p=0.004) and having been on HD for >4 years (p=0.003) were significantly associated with a higher MIS score, whereas living alone (p=0.037) and having been on HD for >4 years (p=0.002) was significantly associated with lower EQ-5D score. We also observed a significant association between the MIS score and the EQ-5D score(r=−0.616, p<0.001). Multiple linear regression analysis demonstrated that diabetic hemodialysis patients who lived within a family household were positively correlated with the QOL score (standardized coefficient, 0.178; 95% confidence interval (CI), 0.042 to 0.372; p = 0.015), and MIS score was significantly and negatively correlated with QOL scores (standardized coefficient, −0.587; 95% CI, −0.047 to −0.028; p < 0.001).

Conclusions
We found that malnutrition was associated with a lower QOL score among diabetic patients on HD. We recommend general practitioners, dietitians, nephrologists, and nurses to make plans that pay more attention to this group of patients who show evidence of malnutrition. Patients on dialysis for ≥ 4 years, patients who live alone, and those suffering from multiple co-morbid diseases should receive special care due to their higher risk of being impacted by this problem.
Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus
Authors: Ma Ziyue , Zhang Hui , Ding Ming , Zhang Zhongkai , Yang Xiuling , Zhou Xueping 
Published: July 2021

AbstractTomato brown rugose fruit virus (ToBRFV) is a new member of the genus Tobamovirus, and has the potential to affect the production and marketability of tomatoes and peppers. In this study, we sequenced and analyzed the complete genome of ToBRFV isolates from tomato plants showing mosaic and mottling symptoms in Yunnan Province of China. We constructed a full-length infectious cDNA clone of ToBRFV, which could induce systemic infection with typical symptoms in tomato, Nicotiana benthamiana, and N. tabacum cv. Samsun nn plants through Agrobacterium-mediated inoculation. Further experimental evidence demonstrated that the rod-shaped virions accumulating in agroinfiltrated plants are sap-transmissible. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of ToBRFV. The system developed herein will facilitate further research on functions of ToBRFV-encoded proteins and plant-ToBRFV interactions through reverse genetic approaches.
Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish
Authors: Duong ThuyVy , Rose Rebecca , Blazeski Adriana , Fine Noah , Woods Courtney E. , Thole Joseph F. , Sotoodehnia Nona , Soliman Elsayed Z. , Tung Leslie , McCallion Andrew S. , Arking Dan E. 
Published: July 2021

ABSTRACT
Clinically pertinent electrocardiogram (ECG) data from model systems, such as zebrafish, are crucial for illuminating factors contributing to human cardiac electrophysiological abnormalities and disease. Current zebrafish ECG collection strategies have not adequately addressed the consistent acquisition of high-quality traces or sources of phenotypic variation that could obscure data interpretation. Thus, we developed a novel platform to ensure high-quality recording of in vivo subdermal adult zebrafish ECGs and zebrafish ECG reading GUI (zERG), a program to acquire measurements from traces that commercial software cannot examine owing to erroneous peak calling. We evaluate normal ECG trait variation, revealing highly reproducible intervals and wave amplitude variation largely driven by recording artifacts, and identify sex and body size as potential confounders to PR, QRS and QT intervals. With this framework, we characterize the effect of the class I anti-arrhythmic drug flecainide acetate on adults, provide support for the impact of a Long QT syndrome model, and establish power calculations for this and other studies. These results highlight our pipeline as a robust approach to evaluate zebrafish models of human cardiac electrophysiological phenotypes.
Advancing lung organoids for COVID-19 research
Authors: van der Vaart Jelte , Lamers Mart M. , Haagmans Bart L. , Clevers Hans 
Published: July 2021

ABSTRACT
The COVID-19 pandemic has emphasised the need to develop effective treatments to combat emerging viruses. Model systems that poorly represent a virus' cellular environment, however, may impede research and waste resources. Collaborations between cell biologists and virologists have led to the rapid development of representative organoid model systems to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We believe that lung organoids, in particular, have advanced our understanding of SARS-CoV-2 pathogenesis, and have laid a foundation to study future pandemic viruses and develop effective treatments.
Cold acclimation induces life stage-specific responses in the cardiac proteome of western painted turtles (Chrysemys picta bellii): implications for anoxia tolerance
Authors: Alderman Sarah L. , Riggs Claire L. , Bullingham Oliver M. N. , Gillis Todd E. , Warren Daniel E. 
Published: July 2021

ABSTRACT
Western painted turtles (Chrysemys picta bellii) are the most anoxia-tolerant tetrapod. Survival time improves at low temperature and during ontogeny, such that adults acclimated to 3°C survive far longer without oxygen than either warm-acclimated adults or cold-acclimated hatchlings. As protein synthesis is rapidly suppressed to save energy at the onset of anoxia exposure, this study tested the hypothesis that cold acclimation would evoke preparatory changes in protein expression to support enhanced anoxia survival in adult but not hatchling turtles. To test this, adult and hatchling turtles were acclimated to either 20°C (warm) or 3°C (cold) for 5 weeks, and then the heart ventricles were collected for quantitative proteomic analysis. The relative abundance of 1316 identified proteins was compared between temperatures and developmental stages. The effect of cold acclimation on the cardiac proteome was only evident in the context of an interaction with life stage, suggesting that ontogenic differences in anoxia tolerance may be predicated on successful maturation of the heart. The main differences between the hatchling and adult cardiac proteomes reflect an increase in metabolic scope with age that included more myoglobin and increased investment in both aerobic and anaerobic energy pathways. Mitochondrial structure and function were key targets of the life stage- and temperature-induced changes to the cardiac proteome, including reduced Complex II proteins in cold-acclimated adults that may help down-regulate the electron transport system and avoid succinate accumulation during anoxia. Therefore, targeted cold-induced changes to the cardiac proteome may be a contributing mechanism for stage-specific anoxia tolerance in turtles.
Pre-analytical considerations in biomarker research: focus on cardiovascular disease
Authors: Revuelta-López Elena , Barallat Jaume , Cserkóová Adriana , Gálvez-Montón Carolina , Jaffe Allan S. , Januzzi James L. , Bayes-Genis Antoni 
Published: July 2021

Abstract
Clinical biomarker research is growing at a fast pace, particularly in the cardiovascular field, due to the demanding requirement to provide personalized precision medicine. The lack of a distinct molecular signature for each cardiovascular derangement results in a one-size-fits-all diagnostic and therapeutic approach, which may partially explain suboptimal outcomes in heterogeneous cardiovascular diseases (e.g., heart failure with preserved ejection fraction). A multidimensional approach using different biomarkers is quickly evolving, but it is necessary to consider pre-analytical variables, those to which a biological sample is subject before being analyzed, namely sample collection, handling, processing, and storage. Pre-analytical errors can induce systematic bias and imprecision, which may compromise research results, and are easy to avoid with an adequate study design. Academic clinicians and investigators must be aware of the basic considerations for biospecimen management and essential pre-analytical recommendations as lynchpin for biological material to provide efficient and valid data.
Discrepancy between emergency department admission diagnosis and hospital discharge diagnosis and its impact on length of stay, up-triage to the intensive care unit, and mortality
Authors: Bastakoti Manish , Muhailan Mohamad , Nassar Ahmad , Sallam Tariq , Desale Sameer , Fouda Ragai , Ammar Hussam , Cole Carmella 
Published: July 2021

Abstract

Objectives
Published discrepancy rates between emergency department (ED) and hospital discharge (HD) diagnoses vary widely (from 6.5 to 75.6%). The goal of this study was to determine the extent of diagnostic discrepancy and its impact on length of hospital stay (LOS), up-triage to the intensive care unit (ICU) and in-hospital mortality.

Methods
A retrospective chart review of adult patients admitted from the ED to a hospitalist service at a tertiary hospital was performed. The ED and HD diagnoses were compared and classified as concordant, discordant, or symptom diagnoses according to predefined criteria. Logistic regression analysis was conducted to examine the associations of diagnostic discordance with in-hospital mortality and up-triage to the ICU. A linear regression model was used for the length of stay.

Results
Of the 636 patients whose records were reviewed, 418 (217 [51.9%] women, with a mean age of 64.1 years) were included. Overall, 318 patients (76%) had concordant diagnoses, while 91 (21.77%) had discordant diagnoses. Only 9 patients (2.15%) had symptom diagnoses. A discordant diagnosis was associated with increased mortality (OR: 3.64; 95% CI: 1.026–12.91; p=0.045) and up-triage to the ICU (OR: 5.51; 95% CI: 2.43–12.5; p<0.001). The median LOS was significantly greater for patients with discordant diagnoses (7 days) than for those with concordant diagnoses (4.7 days) (p=0.004). Symptom diagnosis did not affect the mortality or ICU up-triage.

Conclusions
One in five hospitalized patients had discordant HD and admission diagnoses. This diagnostic discrepancy was associated with significant impacts on patient morbidity and mortality.
Illustrationen zu den Kinder- und Hausmärchen der Brüder Grimm in koreanischen Ausgaben
Authors: Hyun Sook Shin 
Published: July 2021

Zusammenfassung
Dieser Beitrag rekapituliert die Illustrationsgeschichte zu den Kinder- und Hausmärchen in Korea. Nach einem Überblick über drei Phasen (1910 bis 1923, 1945 bis 1989, 1990 bis heute) werden exemplarisch einzelne Illustrationen aus drei Publikationen untersucht, und zwar: 1. aus Dongmyeong
(Ex Oriente Lux), die eine der populärsten Zeitschriften in den frühen 1920er Jahren war; 2. Illustrationen von Hong Seong-Chan aus der 1964 erschienenen Ausgabe Grimms Märchenanthologie, die als Meilenstein der Übersetzungsgeschichte gilt und mit ihren Illustrationen Maßstäbe gesetzt hat. Schließlich werden 3. Illustrationen von Park Eunji vorgestellt, die in der vorbildlichen Ausgabe Die Volkserzählungen der Brüder Grimm (2012) erschienen sind. Ein besonderes Augenmerk legt die Darstellung darauf, Anpassungen an die koreanische Kultur und Lebensweise herauszuarbeiten. Jedoch weist der Forschungsstand immer noch Lücken auf, die auch in diesem Beitrag nicht geschlossen werden können und die vor allem die Zeit von 1924 bis 1945 betreffen.
Image-based 3D reconstruction precision using a camera mounted on a robot arm
Authors: Jezia Yousfi , Samir Lahouar , Abdelmajid Ben Amara 
Published: July 2021

Abstract
In this paper, we study 3D object reconstruction based on a set of 2D images. In order to get the best camera path that increases accuracy we focus on this strategy to be used. Euclidean 3D image-based reconstruction is developed in three steps, which are primitive extraction, correspondence of these primitives and then triangulation. The extraction and triangulation are purely geometrical, whereas the matching step can have precision issues especially in the case of noisy images. An experimental study is carried out where a camera is attached to a robot arm and moved precisely relative to a scene containing a checkerboard calibration pattern. The reconstruction results are compared with values of motion given to the robot. A geometric and analytical study of the impact of the motion of the camera with respect to the scene on the error of a 3D image-based reconstructed point was also carried out. It has been demonstrated that the impact of a correspondence error on the reconstruction accuracy point varies drastically depending on the image capture strategy.
Non-invasive prenatal testing (NIPT): does the practice discriminate against persons with disabilities?
Authors: Dufner Annette 
Published: July 2021

Abstract
The most well-known goal of non-invasive prenatal testing (NIPT) is still to determine whether or not a fetus has trisomy 21. Since women often terminate the pregnancy upon a positive result, there is concern that the use of NIPT contributes to discrimination against persons with disabilities. If this concern is justified, it could have an impact on the wider social acceptability of existing testing practices and their potential further expansion. This paper demonstrates four different versions of the discrimination worry, indicates how international policy papers have reacted to them, and identifies the ethically most relevant feature of the concern.
Inviscid, zero Froude number limit of the viscous shallow water system
Authors: Yang Jianwei , Liu Mengyu , Hao Huiyun 
Published: July 2021

Abstract
In this paper, we study the inviscid and zero Froude number limits of the viscous shallow water system. We prove that the limit system is represented by the incompressible Euler equations on the whole space. Furthermore, the rate of convergence is also obtained.
“It is natural, really deaf signing” – script development for fictional programmes involving sign languages
Authors: Kusters Annelies , Fenlon Jordan 
Published: July 2021

AbstractHistorically, fictional productions which use sign language have often begun with scripts that use the written version of a spoken language. This can be a challenge for deaf actors as they must translate the written word to a performed sign language text. Here, we explore script development inSmall World, a television comedy which attempted to avoid this challenge by using improvisation to create their script. The creators framed this process as a response to what they saw as “inauthentic” sign language use on television, foregrounding the need to present “natural signing” on the screen. According to them, “natural signing” is not influenced by an English script but is varied language use that reflect a character’s background, their settings, and the characters that they interact with. We describe how this authentic language use is derived primarily from improvisation and is in competition with other demands, which are textual (e.g., the need to ensure comedic value), studio-based (e.g., operating within the practical confines of the studio), or related to audience design (e.g., the need to ensure comprehensibility). We discuss how the creative team negotiated the tension between the quest for authentic language use and characteristics of the genre, medium, and audience.
Crystal structure of (1,10-phenanthroline-κ2
N,N′)-bis(3-thiophenecarboxylato-κ2
O,O′)copper(II), C22H14N2O4S2Cu
Authors: Zhang Zhongyu , Men Xiaoxia , Han Bing , Sun Chunyan 
Published: July 2021

Abstract
C22H14N2O4S2Cu, orthorhombic, Pbcn (no. 60), a = 20.171(7) Å, b = 9.684(3) Å, c = 10.372(4) Å, V = 2025.9(12) Å3, Z = 4, R

gt
(F) = 0.0334, wR

ref
(F
2) = 0.0908, T = 273.15 K.