bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-Ultramicronized Palmitoylethanolamide/Luteolin-Induced Oligodendrocyte Precursor Cell Differentiation is Associated With Tyro3 Receptor Upregulation
Authors: Facci Laura , Barbierato Massimo , Fusco Mariella , Giusti Pietro , Zusso Morena 
Published: June 2021

Remyelination in patients with multiple sclerosis frequently fails, especially in the chronic phase of the disease promoting axonal and neuronal degeneration and progressive disease disability. Drug-based therapies able to promote endogenous remyelination capability of oligodendrocytes are thus emerging as primary approaches to multiple sclerosis. We have recently reported that the co-ultramicronized composite of palmitoylethanolamide and the flavonoid luteolin (PEALut) promotes oligodendrocyte precursor cell (OPC) maturation without affecting proliferation. Since TAM receptor signaling has been reported to be important modulator of oligodendrocyte survival, we here evaluated the eventual involvement of TAM receptors in PEALut-induced OPC maturation. The mRNAs related to TAM receptors -Tyro3, Axl, and Mertk- were all present at day 2 in vitro. However, while Tyro3 gene expression significantly increased upon cell differentiation, Axl and Mertk did not change during the first week in vitro. Tyro3 gene expression developmental pattern resembled that of MBP myelin protein. In OPCs treated with PEALut the developmental increase of Tyro3 mRNA was significantly higher as compared to vehicle while was reduced gene expression related to Axl and Mertk. Rapamycin, an inhibitor of mTOR, prevented oligodendrocyte growth differentiation and myelination. PEALut, administered to the cultures 30 min after rapamycin, prevented the alteration of mRNA basal expression of the TAM receptors as well as the expression of myelin proteins MBP and CNPase. Altogether, data obtained confirm that PEALut promotes oligodendrocyte differentiation as shown by the increase of MBP and CNPase and Tyro3 mRNAs as well as CNPase and Tyro3 immunostainings. The finding that these effects are reduced when OPCs are exposed to rapamycin suggests an involvement of mTOR signaling in PEALut effects.
Opening the Schrödinger Box: Short- and Long-Range Mammalian Heart Rate Variability
Authors: Weiser-Bitoun Ido , Davoodi Moran , Rosenberg Aviv A. , Alexandrovich Alexandra , Yaniv Yael 
Published: June 2021

BackgroundThe interactions between the autonomic nervous system (ANS), intrinsic systems (e.g., endocrine), and internal pacemaker mechanisms govern short (milliseconds–seconds)- and long (seconds–minutes)-range heart rate variability (HRV). However, there is a debate regarding the identity of the mechanism underlying HRV on each time scale. We aim to design a general method that accurately differentiates between the relative contribution of the ANS and pacemaker mechanisms to HRV in various mammals, without the need for drug perturbations or organ isolation. Additionally, we aim to explore the universality of the relative contribution of the ANS and pacemaker system of different mammals.MethodsThis work explored short- and long-range HRVs using published ECG data from dogs, rabbits, and mice. To isolate the effects of ANS on HRV, ECG segments recorded before and after ANS-blockade were compared.ResultsDifferentiation of the ANS from extrinsic and intrinsic pacemaker mechanisms was successfully achieved. In dogs, the internal pacemaker mechanisms were the main contributors to long-range and the ANS to short-range HRV. In rabbits and mice, the ANS and the internal pacemaker mechanisms affected both time scales, and anesthesia changed the relative contribution of the pacemaker mechanism to short- and long-range HRVs. In mice, the extrinsic mechanisms affected long-range HRV, while their effect was negligible in rabbits.ConclusionWe offer a novel approach to determine the relative contributions of ANS and extrinsic and intrinsic pacemaker mechanisms to HRV and highlight the importance of selecting mammalian research models with HRV mechanisms representative of the target species of interest.
Physical Activity and Life Satisfaction: An Empirical Study in a Population of Senior Citizens
Authors: Wöbbeking Sánchez Marina , Sánchez Cabaco Antonio , Bonete-López Beatriz , Urchaga Litago José David , Loureiro Manuel Joaquím , Mejía Manuel 
Published: June 2021

Objective: The specialised literature indicates that the two key aspects in active ageing are performing physical activity and life satisfaction. Regarding physical activity, this not only improves physical aspects of senior citizens, but also has a positive impact on mental well-being and satisfaction with one’s own life. The aim is to demonstrate the relationship between these two variables to explain healthy ageing.Method: In a sample of 300 senior citizen subjects, the influence of various sociodemographic variables (age, sex, institutionalisation, and level of education) on the performance of physical activity and life satisfaction, is analysed. The research design is a non-experimental study with two unique cross-sectional and correlational measurement groups.Results: An analysis of the results indicates that people with a higher level of education present differences in physical and motivational reserves. Furthermore, age and institutionalisation have an impact on physical reserves. Analysis using structural equation models allows key relationships between the variables analysed to be predicted, which can guide the implementation of active ageing.Conclusion: Motivational reserves affect healthy cognitive ageing through their positive impact on cognitive and physical reserves.
Unchanged Cognitive Performance and Concurrent Prefrontal Blood Oxygenation After Accelerated Intermittent Theta-Burst Stimulation in Depression: A Sham-Controlled Study
Authors: Struckmann Wiebke , Persson Jonas , Gingnell Malin , Weigl Wojciech , Wass Caroline , Bodén Robert 
Published: June 2021

Aim: Intermittent theta-burst stimulation (iTBS) delivered over the dorsomedial prefrontal cortex (DMPFC) has shown promise as a treatment for anhedonia and amotivation in patients with depression. Here, we investigated whether this protocol modulates cognitive performance and concurrent prefrontal blood oxygenation. We also examined whether depressed patients exhibit cognitive dysfunction and prefrontal hypoactivity at baseline compared to healthy controls.Methods: This sham-controlled study comprises 52 patients randomized to either active or sham accelerated iTBS over the DMPFC (applied twice daily) for 10 consecutive treatment days, and 55 healthy controls. Cognitive performance was assessed at baseline and once again 4 weeks later using a cognitive test battery targeting attention, inhibitory control, and numerical, verbal, and visual working memory. Concurrent prefrontal oxygenated hemoglobin (oxy-Hb) was captured with functional near-infrared spectroscopy.Results: Active iTBS over DMPFC did not affect cognitive performance or concurrent oxy-Hb change compared to sham iTBS in patients with depression. Compared to controls, patients at baseline showed impaired performance in the Trail Making Test, the Rey Auditory Verbal Learning Test, the Animal Naming Test, and the Digit Symbol Substitution Test, however no difference in prefrontal oxy-Hb was observed.Conclusion: Patients with treatment-resistant depression displayed cognitive deficits, however without prefrontal hypoactivity, compared to healthy controls at baseline. iTBS treatment did not alter cognitive performance, nor concurrent prefrontal blood oxygenation, in patients. Taken together, iTBS can likely be considered a cognitively safe treatment option in this sample of patients.
Current antibiotic therapy of endo-perio lesions with secondary endodontic involvement
Authors: Orekhova L. Yu. , Atrushkevitch V. G. , Loboda E. S. , Vashneva V. Yu. , Petrov A. A. 
Published: June 2021

Relevance. Successful treatment of endo-perio lesions depends on the thorough clinical assessment, accurate diagnosis and structural approach to the planning of periodontal and endodontic treatment. Aim – to provide clinical and laboratory evidence about the effectiveness of ciprofloxacin-and-tinidazole-containing antibiotic in the comprehensive treatment of endo-perio lesions with a primary periodontal disease which secondarily affects the pulp.Materials and methods. We examined 29 patients aged from 35 to 50 (mean age 42.3±3.21) with class II endo-perio lesions according to P. Eickholz classification. The patients were randomly divided into 2 groups. All patients had comprehensive periodontal and endodontic treatment with (Group 2) and without (Group 1) antibiotic therapy. The clinical and paraclinical examination included determination of such indices as OHI-s, SL, PMA, SBI, as well as Doppler ultrasound, EPT, CBCT, microbiological test of the discharge from periodontal pockets and root canal system. We selected the systemic antibiotic after the antibiotic sensitivity testing.Results. Comprehensive treatment of endo-perio lesions, which included systemic antibiotic therapy with ciprofloxacin and tinidazole, effectively arrested the inflammation in the periodontium and pulp beginning from the 7th day of use.Conclusion. Cifran ST, a combination broad-spectrum antibiotic, helps reduce periodontal inflammation by effectively eliminating microbiota in the root canals and periodontal pockets in patients with moderate or severe chronic generalized periodontitis.
Comprehensive study of the toothpaste containing fluoride and 67% aqueous sodium bicarbonate solution
Authors: Gromova S. N. , Guzhavina N. A. , Falaleeva E. A. , Kolevatykh E. P. , Elikov А. V. , Petrov S. B. 
Published: June 2021

Relevance. A wide variety of oral care products is available nowadays. Sometimes aggressive advertising rather than doctor’s advice determines our patients’ choice. In our research, we provide evidence of the clinical use of toothpaste containing fluoride and sodium bicarbonate.Materials and methods. During four weeks, we followed up a group of students who used the toothpaste containing 1400 ppm fluoride and 67% aqueous sodium bicarbonate solution. The clinical, biochemical and microbiological tests and saliva crystallization score assessed the characteristics stated by the manufacturer.Results. The statistically significant correlation between all studied criteria is evidence of the effectiveness of the toothpaste. In addition to the significant remineralization and antiplaque effect, biochemical and microbiological tests confirmed the anti-inflammatory effect of the toothpaste. An immediate cleaning effect was observed after the first brushing as well as in long-term use.Conclusion. Improvement of oral hygiene indices and reduction of periodontal inflammation confirmed the successful result of the comprehensive treatment of chronic gingivitis.
Improvement of the prosthodontic treatment protocol in patients with TMD via functional diagnosis
Authors: Dubova L. V. , Prisyazhnykh S. S. , Romankova N. V. , Tagiltsev D. I. , Maksimov G. V. 
Published: June 2021

Relevance. The purpose of the research is to improve the functional diagnosis protocol in prosthodontic treatment of patients with TMD.Materials and methods. The optimal position of the mandible was determined for each patient by two methods: 1) TENS (transcutaneous electrical nerve stimulation) and 2) TENS + kinesiography. Then, the cone-beam computed tomography (CBCT) data were analyzed to determine the most physiological position of the condyles.Results. The analysis of the CT scans of patients without TMD (control group) showed that the right and left condyles occupy an anterior or central symmetrical position relative to the glenoid fossa. In the first and second methods, the condyles occupy an anterior or central position, which is the most optimal position of the lower jaw for the manufacturing of an occlusal stabilization splint. The statistical coefficients allowed us to determine that the second method was more accurate, since the obtained values were lower than those of the first method.Conclusion. Based on the results of this study, we can conclude that the improvement of the protocol, namely a new method for determining the optimal position of the mandible is more time-consuming, but more accurate and allows increasing the effectiveness at all stages of treatment of patients with this pathology.
Comparative analysis of the effectiveness of surgical techniques for the treatment of Miller Class I gingival recessions
Authors: Farkhshatova R. R. , Gerasimova L. P. , Yunusov I. T. 
Published: June 2021

Relevance. It is currently relevant to study and compare the effectiveness of the autologous connective tissue grafts and the combination of collagen-based and autologous platelet-rich plasma in the surgical treatment of Miller Class I gingival recessions.Materials and methods. We examined and treated 48 (20 male (41.67%) and 28 female (58.33%)) patients aged from 25 to 40 years with Miller Class I gingival recessions. All gingival recessions were treated surgically using a modified twolayer tunnel technique. The patients were divided into two groups according to the graft type. Group I (24 patients (50%) had a connective tissue graft from the hard palate. Group II (24 patients (50%) used the combination of the autologous platelet-rich plasma and 3D collagen matrix Fibromatrix for the regeneration of oral soft tissues. We removed the sutures on the 14th day. The patients were followed up on the 7th and 14th days and in 1.3 months.Results. 48 Miller Class I gingival recessions were treated between 2018 and 2020. The depth of gingival recessions averaged 3.5 ± 1.13 mm before treatment. The level of the attached keratinized gingiva regarding the cementoenamel junction significantly (p < 0.001) improved in both groups after the surgery. The width and thickness of the keratinized gingiva best increased in group II. The mean effectiveness of gingival recession treatment was 84% in study group I and 96% – in study group II. Pain syndrome, fibrinous plaque and soft tissue edema were insignificant in group II.Conclusion. The combination of the autologous platelet-rich plasma and Fibromatrix, collagen 3D matrix, for the regeneration of the oral soft tissues is a more effective technique for the treatment of Miller Class I gingival recessions. This technique has several advantages. It is minimally invasive, less painful, soft tissue postoperative swelling is less and the received volume of the attached keratinized gums is larger than with a connective tissue graft.
Characteristics of examination algorithm of patients with chronic trauma of the oral mucosa using tissue autofluorescence spectroscopy
Authors: Gordeeva V. A. , Kulik I. V. , Khromova E. A. , Rubezhov A. L. , Gordeeva M. V. 
Published: June 2021

Relevance. The paper demonstrates the need to implement modern diagnostic techniques for diagnosis of precancerous and cancerous lesions at early or preclinical stages. Additional diagnostic methods are necessary, e.g. tissue autofluorescence, which allows revealing insidious pathological risk zones, particularly precancerous and cancerous lesions, to evaluate the condition of the oral tissues in patients with chronic oral mucosa disorders, especially caused by trauma. Purpose – to assess trauma-specific effectiveness of autofluorescence spectroscopy (AFS) in risk group patients with chronic trauma of the oral mucosa to reveal early malignization signs.Materials and methods. 25 subjects were selected for the study and divided into 2 groups: main group – 20 patients with different manifestations of chronic oral mucosa trauma; control group – 5 subjects without visible clinical manifestations and without oral trauma factors. Autofluorescence spectroscopy was performed in both groups using AFS-400 stomatoscope.Results. The received data demonstrated that the change in autofluorescence doesn’t allow drawing final conclusions on the presence or absence of chronic oral trauma malignization signs.Conclusion. AFS-400 stomatoscope may be effective in differentiating between healthy and damaged tissues, but there is no solid evidence that the change in fluorescence shade can help differentiate between various types of damaged tissues. Autofluorescence spectroscopy should be considered as an additional method for examination of patients with chronic oral mucosa trauma to reveal early malignization signs.
Prospects for the use of fibrin scaffolds populated with pulp and periodontal stem cells: an experimental study
Authors: Dombrovskaya Yu. A. , Enukashvili N. I. , Banashkov R. E. , Semenova N. Yu. , Karabak I. A. , Silin A. V. 
Published: June 2021

Relevance. Creating three-dimensional scaffolds from biodegradable materials and seeding them with stem cells derived from the oral tissues is a promising tool for guided tissue regeneration. Pulp and periodontal stem cells have a high potential for osteogenic differentiation, which biologically determines their use in surgical bone reconstruction. The experiment shows the result of using fibrin glue seeded with pulp and periodontal stem cells on the mandible of laboratory mice. The article presents the results of computed tomography and histological examination. The data provide evidence of the influence of seeded scaffolds on bone remodeling in the area of the defect.Materials and methods. The Local Ethics Committee of the North-Western State Medical University named after I.I. Mechnikov gave permission for the practical part of the research work. The study included 29 white laboratory mice. Molars were extracted and a bone defect was formed. Pulp and periodontal stem cells were obtained and cell-seeded scaffolds were made, then they were introduced into the defect area. The animals were euthanized, maxillofacial CT scan and histology of the defect area were performed 28 days after the molar extraction.Results. The oral cavity of mice was examined, molars were extracted, and teeth were morphologically examined under anesthesia. Scaffolds were synthesized and bone defects were filled. CT scans and histology results were analyzed. The bone volume increased in the main group compared to the control group.Conclusion. The fibrin glue can be used to obtain a material with mechanical characteristics sufficient for a stable shape scaffold. The study proved that the pulp stem cells enclosed in a fibrin glue-based scaffold can maintain the ability to proliferate and osteogenically differentiate. The scaffold based on fibrin glue, which we used, affected the bone remodeling process in the area of jaw defects.
Pemberdayaan Kaum Miskin Sebagai Panggilan Gereja terhadap Masalah Kemiskinan
Authors: Pattiasina Sharon Michelle O. 
Published: June 2021

This article aims to analyze empowerment measures as a church calling on the poverty problems faced by the people.These problems cover various aspects of life, namely education, health, economy and infrastructure including transportation, housing and drinking water.This research uses qualitative methods with interview techniques, observation, documentation, and literature study.The results of the study found that the people of Siahari hamlet experienced poverty in absolute and relative terms.Therefore, in the task and calling of the church, GPM is called to empower people by referring to the theological foundation of Christian faith. In addition, the church can also build cooperation with the government so that empowerment actions can be carried out holistically and produce change for them. AbstrakArtikel ini bertujuan menganalisis tindakan pemberdayaan sebagai panggilan gereja terhadap masalah kemiskinan yang dihadapi oleh umat. Masalah tersebut mencakup berbagai aspek kehidupan, yakni pendidikan, kesehatan, ekonomi dan infrastruktur yang meliputi transportasi, perumahan, dan air minum. Penelitianini menggunakan metode kualitatif dengan teknik wawancara, observasi, dokumenasi, dan studi pustaka.Hasil penelitian menemukan bahwa masyarakat dusun Siahari mengalami kemiskinan secara absolut dan relatif.Oleh sebab itu, dalam tugas dan panggilan gereja maka GPM terpanggil untuk memberddayakan umat dengan mengacu pada landasan teologis iman Kristen. Selain itu, gereja juga dapat membangun kerja sama dengan pemerintah agar tindakan pemberdayaan dapat dilakukan secara holistik dan menghasilkan perubahan bagi mereka.
Evaluasi Penerapan Algoritma Neural Network Sebagai Teknik Reduksi PAPR Pada Sistem OFDM
Authors: Ridwan Mohamad , Gulo Melki Mario , Moegiharto Yoedy , Arifin Arifin , Milchan Muhammad 
Published: June 2021

Pada makalah ini dilakukan evaluasi kinerja algoritma Neural Network sebagai teknik reduksi sistem OFDM. Hasil simulasi untuk sinyal OFDM dengan jumlah subcarrier sebanyak 64 dan modulasi 16 QAM menunjukkan penerapan algorima NN menghasilkan penurunan nilai PAPR sekitar 5,6 dB dari PAPR sinyal OFDM tanpa reduksi. Juga dibandingkan dengan teknik reduksi PAPR metode Iterative Clipping and Filtering, (ICF), Selective Mapping (SLM) dan Partial Transmit Sequence (PTS). Dari kurva CCDF juga ditunjukkan bahwa metode NN menghasilkan kinerja yang lebih baik dibanding metode digabungkan dengan teknik Iterative Clipping and Filtering, (ICF), Selective Mapping (SLM) dan Partial Transmit Sequence (PTS). Evaluasi di sisi penerima dengan pengamatan nilai bit error rate, penerapan algoritma NN memiliki kinerja yang terbaik
Sağlıklı Kent Kavramı için Nüfus Kriterinin Önemi
Authors: DEDE Okan Murat , ŞEKEROĞLU Ahmet 
Published: June 2021

Günümüz itibarıyla uygarlık düzeyi,
kentleşme ile beraber ölçülür bir düzeye gelmiştir. Bugün tüm dünyadaki 8 milyara yakın nüfusun
yaklaşık 6 milyarı kentlerde yaşamaktadır. Bugün, dünyadaki 8 milyara yakın
nüfusun yaklaşık 6 milyarı kentlerde yaşamakta ve nüfusu 1 milyon kişiyi geçen 500
adetten fazla kent bulunmaktadır. Yirminci yüzyıl sonlarında artan çevresel
kirlilik, yok olan doğal ortamlar, küresel iklim değişiklikleri gibi
faktörlerden dolayı, sürdürülebilir bir gelişmenin gerekliliği ortaya koyularak
bunun da çıkış noktasının kentler olduğu kabul edilmiştir. Sağlıklı kent
kavramı da, bu dönemde ortaya çıkan bir kavramdır. Bu bağlamda Dünya Sağlık
Örgütü (DSÖ) 1987 yılında Sağlıklı Şehirler Projesini başlatmıştır. Her yıl
dünya çapında çok sayıda şehir bu proje kapsamında sağlıklı şehirler ulusal ve
uluslararası ağlarına üye olmaktadırlar.
Kuşkusuz bir kenti oluşturan en önemli öge demografik ögedir. Herhangi
bir kentin tüm ekonomik, sosyal ve fiziki şartları nüfus gelişimine göre
değişmektedir. Tüm bu konulara kentte yaşayan toplumun sağlığı da dahildir. Bu makalenin
konusu sağlıklı bir kent olabilmek için nüfus kriterinin önemini ve nüfus
büyüklüğünün sağlıklı kent oluşumundaki etkilerini tartışmaktır. Bu makale
kapsamındaki temel yöntem ise; kentsel gelişme, nüfus ve sağlıklı kent
kavramları üzerinde literatürü aktarabilmek, bu konularla ilgili olarak güncel
rakamları ve mevcut durumu ortaya koyabilmek, bu sayede kentsel nüfusun kent
sağlığı için önemini ortaya koyarak dünya üzerindeki rolünü ve gidişatını
anlamak, ayrıca bazı standartları genelleştirerek nüfus kriterinin sağlıklı
kent olma yolundaki önemini tartışmaya açabilmektir.