bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bosutinib in the real‐life treatment of chronic myeloid leukemia patients aged >65 years resistant/intolerant to previous tyrosine‐kinase inhibitors
Authors: Latagliata Roberto , Attolico Immacolata , Trawinska Malgorzata Monika , Capodanno Isabella , Annunziata Mario , Elena Chiara , Luciano Luigiana , Crugnola Monica , Bergamaschi Micaela , Bonifacio Massimiliano , Baratè Claudia , Mauro Endri , Binotto Gianni , Sgherza Nicola , Aguzzi Chiara , Monteleone Barbara , Sorà Federica , Caocci Giovanni , Luzi Debora , Mariggiò Elena , Scaffidi Luigi , Cattaneo Daniele , Gozzini Antonella , Di Veroli Ambra , Abruzzese Elisabetta , Galimberti Sara , Iurlo Alessandra , Specchia Giorgina , Breccia Massimo 
Published: February 2021

AbstractTo evaluate the role of bosutinib in elderly patients aged >65 years with chronic myeloid leukemia (CML), a real‐life cohort of 101 chronic‐phase CML patients followed up in 23 Italian centers and treated with bosutinib in second or a subsequent line was retrospectively evaluated. Starting dose of bosutinib was 500 mg/day in 25 patients (24.8%), 400 mg/day in 7 patients (6.9%), 300 mg/day in 33 patients (32.7%), 200 mg/day in 34 patients (33.6%), and 100 mg/day in 2 patients (2.0%). Grade 3/4 hematological toxicity occurred in 7/101 patients (6.9%) and grade 3/4 extra‐hematological toxicity in 19/101 patients (18.8%). Permanent bosutinib discontinuation due to toxicity was needed in 12 patients (11.9%). Among the 96 patients evaluable for response, 74 (77.0%) achieved a complete cytogenetic response (CCyR), while 64 of these 74 patients in CCyR (66.6% of all 96 evaluable patients) also achieved a molecular response (MR) (major MR [MR 3.0] in 21 [21.8%], deep MR [MR 4.0/4.5] in 43 [44.8%]). The 3‐year event‐free survival and overall survival of the whole patients' cohort from bosutinib start were 60.9% (CI 95% 49.3–72.5) and 86.4% (CI 95% 77.2–95.6), respectively. Our real‐life data show that bosutinib is effective, with a favorable safety profile, also in elderly patients with important comorbidities and resistance and/or intolerance to previous tyrosine‐kinase inhibitor treatments. As a consequence, it could play a significant role in current clinical practice for frail patients.
A numerical study of flow behavior in the shell and helical finned‐tube heat exchanger
Authors: Dehghani Hamed , Farhadi Mousa , Darzi A. Ali Rabienataj , Kardgar Amin 
Published: February 2021

AbstractExtended surfaces mostly aim to improve the heat transfer upon increasing the area of heat transfer. In this paper, the influence of using fins on flow behaviors and the heat transfer of the shell and tube heat exchanger has been investigated. In this regard, the present results are verified with available experimental data in the literature for a helical tube without fins. The effects of fin density (fin per inch), its height, and material have been studied on the heat transfer rate. In addition, the effects of radial pitch and the number of coil loops are studied. The results indicate that implementing extended surfaces significantly increases the heat transfer rate. The increase of fin density from 8 to 12 and the height from 11.5 to 13.5 mm enhances heat transfer up to 48% and 43% depending on Dean number, respectively. The rise of coil pitch augments the overall heat transfer, and it is more efficient at lower Dean numbers. The predicted results also show that the fin material does not have any significant effect on heat transfer.
To the fast calculation of the solvation free energy. Combining expanded ensembles with L2MC
Authors: Nikitin Alexei , Milchevskaya Vladislava , Lyubartsev Alexander 
Published: February 2021

AbstractA more efficient version of the Expanded Ensembles method for calculation of free energy in molecular‐mechanical simulations is proposed. The method is based on the Horowitz L2MC approach to accelerate movement along the “alchemical” coordinate. It is possible to achieve the same efficiency of the algorithm both with the optimal number of “windows” and with a larger number of them compared to the original algorithm. Since the optimal number of windows is unknown a priory, the proposed algorithm is more robust than the traditional one. We can choose the number of windows in excess and do not worry about the loss of efficiency. We illustrate the method's efficiency with the computation of the hydration free energies of pyridine and water.
The low molecular weight protein tyrosine phosphatase promotes adipogenesis and subcutaneous adipocyte hypertrophy
Authors: Stanford Stephanie M. , Collins Meghan , Diaz Michael A. , Holmes Zachary J. , Gries Paul , Bliss Matthew R. , Lodi Alessia , Zhang Vida , Tiziani Stefano , Bottini Nunzio 
Published: February 2021

AbstractObesity is a major contributing factor to the pathogenesis of Type 2 diabetes. Multiple human genetics studies suggest that high activity of the low molecular weight protein tyrosine phosphatase (LMPTP) promotes metabolic syndrome in obesity. We reported that LMPTP is a critical promoter of insulin resistance in obesity by regulating liver insulin receptor signaling and that inhibition of LMPTP reverses obesity‐associated diabetes in mice. Since LMPTP is expressed in adipose tissue but little is known about its function, here we examined the role of LMPTP in adipocyte biology. Using conditional knockout mice, we found that selective deletion of LMPTP in adipocytes impaired obesity‐induced subcutaneous adipocyte hypertrophy. We assessed the role of LMPTP in adipogenesis in vitro, and found that LMPTP deletion or knockdown substantially impaired differentiation of primary preadipocytes and 3T3‐L1 cells into adipocytes, respectively. Inhibition of LMPTP in 3T3‐L1 preadipocytes also reduced adipogenesis and expression of proadipogenic transcription factors peroxisome proliferator activated receptor gamma (PPARγ) and CCAAT/enhancer‐binding protein alpha. Inhibition of LMPTP increased basal phosphorylation of platelet‐derived growth factor receptor alpha (PDGFRα) on activation motif residue Y849 in 3T3‐L1, resulting in increased activation of the mitogen‐associated protein kinases p38 and c‐Jun N‐terminal kinase and increased PPARγ phosphorylation on inhibitory residue S82. Analysis of the metabolome of differentiating 3T3‐L1 cells suggested that LMPTP inhibition decreased cell glucose utilization while enhancing mitochondrial respiration and nucleotide synthesis. In summary, we report a novel role for LMPTP as a key driver of adipocyte differentiation via control of PDGFRα signaling.
High performance of a novel antigen detection test on nasopharyngeal specimens for diagnosing SARS‐CoV‐2 infection
Authors: Courtellemont L. , Guinard J. , Guillaume C. , Giaché S. , Rzepecki V. , Seve A. , Gubavu C. , Baud K. , Le Helloco C. , Cassuto G. N. , Pialoux G. , Hocqueloux L. , Prazuck T. 
Published: February 2021

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic has become a major public health issue worldwide. Developing and evaluating rapid and easy‐to‐perform diagnostic tests is a high priority. The current study was designed to assess the diagnostic performance of an antigen‐based rapid detection test (COVID‐VIRO®) in a real‐life setting. Two nasopharyngeal specimens of symptomatic or asymptomatic adult patients hospitalized in the Infectious Diseases Department or voluntarily accessing the COVID‐19 Screening Department of the Regional Hospital of Orléans, France, were concurrently collected. The diagnostic specificity and sensitivity of COVID VIRO® results were compared to those of real‐time reverse‐transcriptase quantitative polymerase chain reaction (RT‐qPCR) results. A subset of patients underwent an additional oropharyngeal and/or saliva swab for rapid testing. A total of 121 patients confirmed to be infected and 127 patients having no evidence of recent or ongoing infection were enrolled for a total of 248 nasopharyngeal swab specimens. Overall, the COVID‐VIRO® sensitivity was 96.7% (CI, 93.5%–99.9%). In asymptomatic patients, symptomatic patients having symptoms for more than 4 days and those with an RT‐qPCR cycle threshold value ≥ 32, the sensitivities were 100%, 95.8%, and 91.9%, respectively. The concordance between RT‐qPCR and COVID VIRO® rapid test results was 100% for the 127 patients with no SARS‐CoV‐2 infection. The COVID‐VIRO® test had 100% specificity and sensitivity greater than 95%, which are better than the recommendations set forth by the WHO (specificity ≥ 97%–100%, sensitivity ≥ 80%). These rapid tests may be particularly useful for large‐scale screening in emergency departments, low‐resource settings, and airports.
Overexpression of the ascorbate peroxidase through enhancer‐trapped pSB111 bar vector for alleviating drought stress in rice
Authors: Gorripati Srinivas , Konka Rajasekhar , Panditi Shravana Kumar , Velagapudi Kavitha , Jeevigunta Naveena Lavanya Latha 
Published: February 2021

AbstractRice (Oryza sativa L.) plant growth and productivity is adversely affected by various stress factors. Overexpression of drought tolerance‐related genes is one of the best approaches for developing drought‐resistant transgenics. Agrobacterium tumefaciens has been widely used in generating transgenic plants through plasmid vector to obtain desired characteristics and to know the specific expression profiles of genes in the plant. The enhancer trap method was developed to know the specific expression of genes at different stages of growth by entrapping the genes of an organism. In the present study, we designed a vector molecule with a feature of promoting the expression of a specific gene more than four times than its normal expression and it is useful for efficient transformation to higher plants by utilizing the trans configuration of vir genes of the plasmid A. tumefaciens, to transfer right and left sequence bordered of transferred DNA (T‐DNA) into the nuclear genome of plants. We developed a binary vector consisting of 1.8‐kb green fluorescent protein (GFP) cassette as a reporter gene and 1.4‐kb tetramer of CaMv35S enhancer (4XEn) were cloned at HindIII site of pSB11 bar intermediate vector to tag and know the genes and their expression profiles, then mobilized into A. tumefaciens to produce a super‐binary vector pSB111‐bar‐4XEn‐GFP. The resultant construct was confirmed by polymerase chain reaction and restriction digestion methods. Finally, we discuss the role of overexpressed ascorbate peroxidase in drought stress.
Climatological characterization of tropical cyclones detected in the regional climate simulations over the CORDEX‐SEA domain
Authors: Tibay Jennifer , Cruz Faye , Tangang Fredolin , Juneng Liew , Ngo‐Duc Thanh , Phan‐Van Tan , Santisirisomboon Jerasorn , Singhruck Patama , Gunawan Dodo , Aldrian Edvin , Narisma Gemma Teresa 
Published: February 2021

AbstractIn this study, a subset of the downscaled simulations of the Southeast Asia Regional Climate Downscaling/Coordinated Regional Climate Downscaling Experiment—Southeast Asia (SEACLID/CORDEX‐SEA) was analysed to examine the representation of tropical cyclone (TC) climatology over Southeast Asia, in terms of pattern, intensity, frequency, and lifetime. A modified vortex tracking algorithm is used to detect TCs over the SEACLID/CORDEX‐SEA domain in the historical simulations from 1986 to 2005. Sensitivity tests for the detection method criteria, including vorticity, outer core wind strength, sea level pressure anomaly, and temperature anomaly at 300, 500, 700, and 850 hPa, were conducted to determine the optimum threshold configuration for each SEACLID/CORDEX‐SEA simulation used in the study. Comparison with the best track data of the Joint Typhoon Warning Center showed that model simulations underestimated the total number of TCs east of the Philippines for the 1986–2005 period but captured the annual cycle of the total number of TCs. This underestimation of TCs is possibly due to the domain used, which does not extend further east to cover most of the TC genesis area in the Western North Pacific. The structure of a typical TC from the regional climate model simulation is comparable to observed TC structure. However, results indicate that the resolution of the simulations is still not sufficient to capture the fine details of the observed TC structure, which could explain in part the lower intensification rate of TCs in the model output.
Exploring the extent to which fluctuations in ice‐rafted debris reflect mass changes in the source ice sheet: a model–observation comparison using the last British–Irish Ice Sheet
Authors: Wilton David J. , Bigg Grant R. , Scourse James D. , Ely Jeremy C. , Clark Chris D. 
Published: February 2021

ABSTRACTThe British and Irish Ice Sheet (BIIS) was highly dynamic during the Late Quaternary, with considerable regional differences in the timing and extent of its change. This was reflected in equally variable offshore ice‐rafted debris (IRD) records. Here we reconcile these two records using the FRUGAL intermediate complexity iceberg–climate model, with varying BIIS catchment‐level iceberg fluxes, to simulate change in IRD origin and magnitude along the western European margin at 1000‐year time steps during the height of the last BIIS glaciation (31–6 ka bp). This modelled IRD variability is compared with existing IRD records from the deep ocean at five cores along this margin. There is general agreement of the temporal and spatial IRD variability between observations and model through this period. The Porcupine Bank off northwestern Ireland was confirmed by the modelling as a major dividing line between sites possessing exclusively northern or southern source regions for offshore IRD. During Heinrich events 1 and 2, the cores show evidence of a proportion of North American IRD, more particularly to the south of the British Isles. Modelling supports this southern bias for likely Heinrich impact, but also suggests North American IRD will only reach the British margin in unusual circumstances.
A process‐based soil erosion model ensemble to assess model uncertainty in climate‐change impact assessments
Authors: Eekhout Joris P.C. , Millares‐Valenzuela Agustín , Martínez‐Salvador Alberto , García‐Lorenzo Rafael , Pérez‐Cutillas Pedro , Conesa‐García Carmelo , de Vente Joris 
Published: February 2021

AbstractThe impact of climate change on future soil loss is commonly assessed with soil erosion models, which are suggested to be an important source of uncertainty. Here, we present a novel soil erosion model ensemble to assess model uncertainty in climate‐change impact assessments. The model ensemble consists of five continuous process‐based soil erosion models that run at a daily time step (i.e., DHSVM, HSPF, INCA, MMF, SHETRAN). The models were implemented in the SPHY hydrological model and simulate detachment by raindrop impact, detachment by runoff, and immediate deposition. The soil erosion model ensemble was applied in a semiarid catchment in the southeast of Spain. We applied three future climate scenarios based on global mean temperature rise (+1.5, +2 and +3°C). Data from two contrasting regional climate models were used to assess how an increase and a decrease in projected extreme precipitation affect model uncertainty. Soil loss is projected to increase (up to 95%) and decrease (up to −30%) under climate change, mostly reflecting the change in extreme precipitation. Model uncertainty is found to increase with increasing slope, extreme precipitation and runoff, which reveals some inherent differences in model assumptions among the five models. Moreover, the model uncertainty increases in all climate change scenarios, independent of the projected change in annual precipitation and extreme precipitation. This stresses the importance to consider model uncertainty through model ensembles of climate, hydrology, and soil erosion in climate‐change impact assessments.
Self‐Healing Polyurethane Elastomer Based on Crosslinked‐Linear Interpenetrating Structure: Preparation, Properties, and Applications
Authors: Li Shaonan , Liu Mengyue , Zhou Xing , Dong Yanmao , Li Jihang 
Published: February 2021

AbstractDespite tremendous advances in the preparation of self‐healing flexible strain sensor devices, it remains a great challenge to large‐area synthesis, relatively high mechanical strength, and sensitive sensing properties into one self‐healing materials system, which have received increasing interests because of their many potential applications. Herein, the design and synthesis of a crosslinked‐linear interpenetrating structure polymer containing disulfide and hydrogen bonds is reported to address this conundrum. The resulting self‐healable polymer is highly stretchable (up to 551.7%) with a high tensile strength (4.14 MPa) and excellent healing efficiency of similar to 90% at mild temperature without using any external reagents. Furthermore, a novel method for fabricating flexible sensor is also proposed to endow the resulted sensor with large‐area (20 cm × 12 cm), low cost, and outstanding sensitivity to strain, which makes it very suitable for human motion monitoring applications. This work will provide afflatus on future design, fabrication, and application of self‐healing flexible strain sensor devices.
Local well‐posedness for an isentropic compressible Ginzburg–Landau–Navier–Stokes with vacuum
Authors: Fan Jishan , Hu Yuxi , Nakamura Gen 
Published: February 2021

AbstractIn this work, we prove the local well‐posedness of local strong solutions to an isentropic compressible Ginzburg–Landau–Navier–Stokes system with vacuum in a bounded domain .
Four‐dimensional gradient almost Ricci solitons with harmonic Weyl curvature
Authors: Kim Jongsu 
Published: February 2021

AbstractIn this article we make a classification of four‐dimensional gradient almost Ricci solitons with harmonic Weyl curvature. We prove first that any four‐dimensional (not necessarily complete) gradient almost Ricci soliton with harmonic Weyl curvature has less than four distinct Ricci‐eigenvalues at each point. If it has three distinct Ricci‐eigenvalues at each point, then is locally a warped product with 2‐dimensional base in explicit form, and if g is complete in addition, the underlying smooth manifold is or . Here is a smooth surface admitting a complete Riemannian metric with constant curvature k. If has less than three distinct Ricci‐eigenvalues at each point, it is either locally conformally flat or locally isometric to the Riemannian product , , where has the Euclidean metric and is a 2‐dimensional Riemannian manifold with constant curvature λ. We also make a complete description of four‐dimensional gradient almost Ricci solitons with harmonic curvature.
Synthesis and characterization of chemically crosslinked gelatin and chitosan to produce hydrogels for biomedical applications
Authors: Joshi Prutha , Ahmed Md Shakir Uddin , Vig Komal , Vega Erramuspe Iris Beatriz , Auad Maria L. 
Published: February 2021

AbstractGelatin and chitosan polysaccharides were chemically modified to get methacrylate functionality to obtain biocompatible hydrogels for use as tissue engineering scaffolds. The methacrylation reaction was verified by 1H‐NMR. The degree of methacrylation was varied from 7% to 40% by changing the molar ratio of polysaccharide to methacrylic anhydride and the type of polysaccharide utilized. After the modification, polysaccharide‐based hydrogels were prepared by free‐radical polymerization in the presence of UV light and Irgacure 184 as a photoinitiator. The physical, chemical, and mechanical performances of the hydrogels were further characterized. Also, the biodegradability and the viability of the polysaccharide hydrogels were investigated using fibroblast cells. These cells were seeded directly onto the hydrogel surface, populated the entirety of the hydrogel, and remained viable for up to 1 week. Altogether, the modified polysaccharides exhibit the properties which make them crucial for applications in the field of regenerative medicine.
Polyhedral oligomeric silsesquioxane hybrided with DOPO and phenylboronic acid for flame‐retarded epoxy resin
Authors: Birong Zeng , Ruirui Zhou , Xianghong Zheng , Jianyu Ye , Jinmei Chen , Yiting Xu , Conghui Yuan , Lizong Dai 
Published: February 2021

AbstractA new polyhedral oligomeric silsesquioxane (POSS) derivate bearing phenylboronic acid group and 6H‐dibenzo[c,e][1,2]oxaphosphinine‐6‐oxide (DOPO) unit was synthesized and applied in flame retardant epoxy resin (EP). Compared to pure EP, EP/POSS‐DOPO‐(phenylboronic acid) composites had good transparency and better thermal stability. Micro‐scale combustion calorimetry results showed that the addition of POSS‐DOPO‐(phenylboronic acid) hybrid could greatly reduce the PHRR and THR of EP/POSS‐DOPO‐(phenylboronic acid) composite. Simultaneously, the UL‐94 of EP composite with 7% POSS‐DOPO‐(phenylboronic acid) achieved V‐0 grade and the LOI value increased from 19.4% (neat EP) to 28.6%. Raman spectra results suggested that POSS‐DOPO‐(phenylboronic acid) hybrid enhanced the formation of residual char with graphitic structure during combustion. Besides, SEM characterization showed that the char of EP/POSS‐DOPO‐(phenylboronic acid) composite was much more continuous and compact in comparison with that of control samples. Moreover, the mechanical and dielectric properties of EP/POSS‐DOPO‐(phenylboronic acid) composites have been greatly improved for a better application.
The Hybrid Autocollimator
Authors: Aharon Oren , Vishnia Itai 
Published: February 2021

AbstractThe concept of autocollimation as an optical instrument was conceived about a century ago for accurate, non‐contact measurements of angles. Since it was invented, it has developed a long history of being used in alignment of angles and optical elements. Recent novel photonics development has created a need for alignment and measurement of optics and lasers – the new hybrid technology does exactly that.
Flow Cytometry for Immunology Research
Authors: Waldeck Ingo , Schulze Matthias 
Published: February 2021

AbstractThe fight against the current Covid‐19 pandemic highlights the need for, and benefits of ‘personalized medicine’. This is driving a growing demand for more detailed analysis of disease pathology as well as patients' individual immune system characteristics and hence predicted disease progression and optimum treatment. One of the consequences is that clinical labs and research facilities are looking to increase performance and usage of multiparameter flow cytometry as a key tool to analyze the cellular components of the immune system. In response, Coherent is supporting faster instrument development and increasing the number of parameters in two ways – integrated multiwavelength OEM subsystems, and new laser wavelengths in the ultraviolet, all with output beam parameters optimized for common flow cytometry platforms.
MIR Spectroscopy for Continuous Control and Monitoring of Metabolites
Authors: Baekelandt Géraldine , Müller Anja 
Published: February 2021

AbstractA new measurement system, which is based on mid‐infrared spectroscopy, allows to monitor multiple bioprocesses online at the same time. Applications of the instrument are foreseen in upstream bioprocessing, in metabolite monitoring and control, as well as in downstream bioprocessing to aid in aggregation studies, contaminant detection or the monitoring of target proteins and excipients.