bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lyell, the Geikies and Croll's observations on terrestrial glacial sediments and landforms
Authors: ROSE James 
Published: June 2021

ABSTRACTWithin the context of the work and achievements of James Croll, this paper reviews the records of direct observations of glacial landforms and sediments made by Charles Lyell, Archibald and James Geikie and James Croll himself, in order to evaluate their contributions to the sciences of glacial geology and Quaternary environmental change. The paper outlines the social and physical environment of Croll's youth and contrasts this with the status and experiences of Lyell and the Geikies. It also outlines the character and role of the ‘Glasgow School’ of geologists, who stimulated Croll's interest into the causes of climate change and directed his focus to the glacial and ‘interglacial’ deposits of central Scotland. Contributions are outlined in chronological order, drawing attention to: (i) Lyell's high-quality observations and interpretations of glacial features in Glen Clova and Strathmore and his subsequent rejection of the glacial theory in favour of processes attributed to floating icebergs; (ii) the significant impact of Archibald Geikie's 1863 paper on the ‘glacial drift of Scotland’, which firmly established the land-ice theory; (iii) the fact that, despite James Croll's inherent dislike of geology and fieldwork, he provided high-quality descriptions and interpretations of the landforms and sediments of central Scotland in order to test his theory of climate change; and (iv) the great communication skills of James Geikie, enhanced by contacts and evidence from around the world. It is concluded that whilst direct observations of glacial landforms and sediments were critical to the long-term development of the study of glaciation, the acceptance of this theory was dependent also upon the skills, personality and status of the Geikies and Croll, who developed and promoted the concepts. Sadly, the subsequent rejection of the land-ice concept by Lyell resulted in the same factors challenging the acceptance of the glacial theory.
Science, metaphysics and Calvinism: the God of James Croll
Authors: FINNEGAN Diarmid A. 
Published: June 2021

ABSTRACTScience, for James Croll, began and ended in metaphysics. Metaphysics, in turn, provided proof of a First and Final Cause of all things. This proof rested on two metaphysical principles: that every event must have a cause, and that the determination of a cause is distinct from its production. This argument emerged from his deeply held religious commitments. As a 17-year-old, he converted to a Calvinist and evangelical form of Christianity. After a period of questioning the Calvinist system, he embraced it again through reading the famous treatise on the will by the New England theologian, Jonathan Edwards. This determinedly metaphysical work, which engaged as much with Enlightenment thought as with Calvinism, defended the view that the will was not a self-determining cause of human action. This ‘hard case’ provided the basis for a larger claim that every act whatever has a cause, and that the production of an act was different from its determination. In part through reading Edwards, Croll remained a devout and convinced ‘moderate’ Calvinist for the rest of his life. He also developed a deep love of metaphysics and became convinced that without it, everything, including science, remained confused and in darkness. For Croll, even the most basic science could not be properly conducted without prior metaphysical principles. But this was more than just an argument about the philosophical foundations of scientific inquiry. It was also based on Croll's conviction that the cosmos, earth history and life (including his own) was fully determined by a supreme and perfect intellect. This conviction entered into the marrow of Croll's scientific theories and shaped his interpretation of the twists and turns of his own life. In short, to take seriously Croll's own self-understanding, we need to allow him to ‘do God’.
Gender quotas and public demand for increasing women’s representation in politics: an analysis of 28 European countries
Authors: Fernández Juan J. , Valiente Celia 
Published: June 2021

AbstractFemale representation in political decision-making positions is now a salient issue in public discussions throughout Europe. Understanding public attitudes towards a more balanced gender distribution in politics remains limited, however. Using a 2017 Eurobarometer, we focus on cross-national differences in public support for increased female participation in politics to address this limitation. Building on the policy feedbacks literature, we stress the role of gender quotas. We argue that quotas – as legislative devices usually adopted through elite-driven initiatives – stimulate support for stronger female representation. Ensuing debates on quotas raise individual awareness about the underrepresentation of women – informational effect – and, once adopted, give a clear signal that persistent gender imbalance is a social problem to be redressed – normative effect. Our empirical analysis supports this argument. Citizens in countries with gender quotas display stronger support for increased female participation in politics.
CRISPR/Cas9-mediated targeted mutation reveals a role for AN4 rather than DPL in regulating venation formation in the corolla tube of Petunia hybrida
Authors: Zhang Bin , Xu Xiaojing , Huang Renwei , Yang Sha , Li Mingyang , Guo Yulong 
Published: June 2021

AbstractVenation is a common anthocyanin pattern displayed in flowers that confers important ornamental traits to plants. An anthocyanin-related R2R3-MYB transcription factor, DPL, has been proposed to regulate corolla tube venation in petunia plants. Here, however, we provide evidence redefining the role of DPL in petunia. A CRISPR/Cas9-mediated mutation of DPL resulted in the absence of the vein-associated anthocyanin pattern above the abaxial surface of the flower bud, but not corolla tube venation, thus indicating that DPL did not regulate the formation of corolla tube venation. Alternately, quantitative real-time PCR analysis demonstrated that the spatiotemporal expression pattern of another R2R3-MYB gene, AN4, coincided with the formation of corolla tube venation in petunia. Furthermore, overexpression of AN4 promoted anthocyanin accumulation by increasing the expression of anthocyanin biosynthesis genes. CRISPR/Cas9-mediated mutation of AN4 led to an absence of corolla tube venation, suggesting that this gene in fact determines this key plant trait. Taken together, the results presented here redefine the prime regulator of corolla tube venation, paving the way for further studies on the molecular mechanisms underlying the various venation patterns in petunia.
The diverse roles of cytokinins in regulating leaf development
Authors: Wu Wenqi , Du Kang , Kang Xiangyang , Wei Hairong 
Published: June 2021

AbstractLeaves provide energy for plants, and consequently for animals, through photosynthesis. Despite their important functions, plant leaf developmental processes and their underlying mechanisms have not been well characterized. Here, we provide a holistic description of leaf developmental processes that is centered on cytokinins and their signaling functions. Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the initial stage of leaf formation; cytokinins and auxins, as well as their interaction, determine the phyllotaxis pattern. The activities of cytokinins in various regions of the leaf, especially at the margins, collectively determine the final leaf morphology (e.g., simple or compound). The area of a leaf is generally determined by the number and size of the cells in the leaf. Cytokinins promote cell division and increase cell expansion during the proliferation and expansion stages of leaf cell development, respectively. During leaf senescence, cytokinins reduce sugar accumulation, increase chlorophyll synthesis, and prolong the leaf photosynthetic period. We also briefly describe the roles of other hormones, including auxin and ethylene, during the whole leaf developmental process. In this study, we review the regulatory roles of cytokinins in various leaf developmental stages, with a focus on cytokinin metabolism and signal transduction processes, in order to shed light on the molecular mechanisms underlying leaf development.
Tissue and regional expression patterns of dicistronic tRNA–mRNA transcripts in grapevine (Vitis vinifera) and their evolutionary co-appearance with vasculature in land plants
Authors: Fabres Pastor Jullian , Anand Lakshay , Sai Na , Pederson Stephen , Zheng Fei , Stewart Alexander A. , Clements Benjamin , Lampugnani Edwin R. , Breen James , Gilliham Matthew , Tricker Penny , Rodríguez López Carlos M. , David Rakesh 
Published: June 2021

AbstractTransfer RNAs (tRNA) are crucial adaptor molecules between messenger RNA (mRNA) and amino acids. Recent evidence in plants suggests that dicistronic tRNA-like structures also act as mobile signals for mRNA transcripts to move between distant tissues. Co-transcription is not a common feature in the plant nuclear genome and, in the few cases where polycistronic transcripts have been found, they include non-coding RNA species, such as small nucleolar RNAs and microRNAs. It is not known, however, the extent to which dicistronic transcripts of tRNA and mRNAs are expressed in field-grown plants, or the factors contributing to their expression. We analysed tRNA–mRNA dicistronic transcripts in the major horticultural crop grapevine (Vitis vinifera) using a novel pipeline developed to identify dicistronic transcripts from high-throughput RNA-sequencing data. We identified dicistronic tRNA–mRNA in leaf and berry samples from 22 commercial vineyards. Of the 124 tRNA genes that were expressed in both tissues, 18 tRNA were expressed forming part of 19 dicistronic tRNA–mRNAs. The presence and abundance of dicistronic molecules was tissue and geographic sub-region specific. In leaves, the expression patterns of dicistronic tRNA–mRNAs significantly correlated with tRNA expression, suggesting that their transcriptional regulation might be linked. We also found evidence of syntenic genomic arrangements of tRNAs and protein-coding genes between grapevine andArabidopsis thaliana, and widespread prevalence of dicistronic tRNA–mRNA transcripts among vascular land plants but no evidence of these transcripts in non-vascular lineages. This suggests that the appearance of plant vasculature and tRNA–mRNA occurred concurrently during the evolution of land plants.
Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato
Authors: Sierra-Orozco Edgar , Shekasteband Reza , Illa-Berenguer Eudald , Snouffer Ashley , van der Knaap Esther , Lee Tong Geon , Hutton Samuel F. 
Published: June 2021

AbstractWithin large-fruited germplasm, fruit size is influenced by flat and globe shapes. Whereas flat fruits are smaller and retain better marketability, globe fruits are larger and more prone to cuticle disorders. Commercial hybrids are often developed from crosses between flat and globe shaped parents because flat shape is thought to be dominant and fruit size intermediate. The objectives of this study were to determine the genetic basis of flat/globe fruit shape in large-fruited fresh-market tomato germplasm and to characterize its effects on several fruit traits. Twenty-three advanced single plant selections from the Fla. 8000 × Fla. 8111B cross were selectively genotyped using a genome-wide SNP array, and inclusive composite interval mapping identified a single locus on the upper arm of chromosome 12 associated with shape, which we termed globe. A 238-plant F2 population and 69 recombinant inbred lines for this region from the same parents delimited globe to approximately 392-kilobases. A germplasm survey representing materials from multiple breeding programs demonstrated that the locus explains the flat/globe shape broadly. A single base insertion in an exon of Solyc12g006860, a gene annotated as a brassinosteroid hydroxylase, segregated completely with shape in all populations tested. CRISPR/Cas9 knock out plants confirmed this gene as underlying the globe locus. In silico analysis of the mutant allele of GLOBE among 595 wild and domesticated accessions suggested that the allele arose very late in the domestication process. Fruit measurements in three genetic backgrounds evidenced that globe impacts fruit size and several fruit shape attributes, pedicel length/width, and susceptibility of fruit to weather check. The mutant allele of GLOBE appears mostly recessive for all traits except fruit size where it acts additively.
A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses
Authors: Hardy Joshua M. , Newton Natalee D. , Modhiran Naphak , Scott Connor A. P. , Venugopal Hariprasad , Vet Laura J. , Young Paul R. , Hall Roy A. , Hobson-Peters Jody , Coulibaly Fasséli , Watterson Daniel 
Published: June 2021

AbstractThe epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5  Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.
Quantum surface-response of metals revealed by acoustic graphene plasmons
Authors: Gonçalves P. A. D. , Christensen Thomas , Peres Nuno M. R. , Jauho Antti-Pekka , Epstein Itai , Koppens Frank H. L. , Soljačić Marin , Mortensen N. Asger 
Published: June 2021

AbstractA quantitative understanding of the electromagnetic response of materials is essential for the precise engineering of maximal, versatile, and controllable light–matter interactions. Material surfaces, in particular, are prominent platforms for enhancing electromagnetic interactions and for tailoring chemical processes. However, at the deep nanoscale, the electromagnetic response of electron systems is significantly impacted by quantum surface-response at material interfaces, which is challenging to probe using standard optical techniques. Here, we show how ultraconfined acoustic graphene plasmons in graphene–dielectric–metal structures can be used to probe the quantum surface-response functions of nearby metals, here encoded through the so-called Feibelman d-parameters. Based on our theoretical formalism, we introduce a concrete proposal for experimentally inferring the low-frequency quantum response of metals from quantum shifts of the acoustic graphene plasmons dispersion, and demonstrate that the high field confinement of acoustic graphene plasmons can resolve intrinsically quantum mechanical electronic length-scales with subnanometer resolution. Our findings reveal a promising scheme to probe the quantum response of metals, and further suggest the utilization of acoustic graphene plasmons as plasmon rulers with ångström-scale accuracy.
Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis
Authors: Horisawa-Takada Yuki , Kodera Chisato , Takemoto Kazumasa , Sakashita Akihiko , Horisawa Kenichi , Maeda Ryo , Shimada Ryuki , Usuki Shingo , Fujimura Sayoko , Tani Naoki , Matsuura Kumi , Akiyama Tomohiko , Suzuki Atsushi , Niwa Hitoshi , Tachibana Makoto , Ohba Takashi , Katabuchi Hidetaka , Namekawa Satoshi H. , Araki Kimi , Ishiguro Kei-Ichiro 
Published: June 2021

AbstractDuring spermatogenesis, meiosis is accompanied by a robust alteration in gene expression and chromatin status. However, it remains elusive how the meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identify a protein complex that consists of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulators in mouse meiotic prophase progression. Our genetic study shows that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of the meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses identify that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrates that a germ-cell specific complex that contains ZFP541 and KCTD19 promotes the progression of meiotic prophase towards completion in male mice, and triggers the reconstruction of the transcriptional network and chromatin organization leading to post-meiotic development.
The impact of lymphedema on health-related quality of life up to 10 years after breast cancer treatment
Authors: Jørgensen Mads G. , Toyserkani Navid M. , Hansen Frederik G. , Bygum Anette , Sørensen Jens A. 
Published: June 2021

AbstractThe impact of breast cancer-related lymphedema (BCRL) on long-term quality of life is unknown. The aim of this study was to investigate the impact of BCRL on health-related quality of life (HRQoL) up to 10 years after breast cancer treatment. This regional population-based study enrolled patients treated for breast cancer with axillary lymph node dissection between January 1st 2007 and December 31th 2017. Follow up and assessments of the included patients were conducted between January 2019 and May 2020. The study outcome was HRQoL, evaluated with the Lymphedema Functioning, Disability and Health Questionnaire, the Disabilities of the Arm, Shoulder and Hand Questionnaire and the Short Form (36) Health Survey Questionnaire. Multivariate linear logistic regression models adjusted for confounders provided mean score differences (MDs) with 95% confidence intervals in each HRQoL scale and item. This study enrolled 244 patients with BCRL and 823 patients without BCRL. Patients with BCRL had significantly poorer HRQoL than patients without BCRL in 16 out of 18 HRQoL subscales, for example, in physical function (MDs 27, 95%CI: 24; 30), mental health (MDs 24, 95%CI: 21; 27) and social role functioning (MDs 20, 95%CI: 17; 23). Age, BMI, BCRL severity, hand and dominant arm affection had only minor impact on HRQoL (MDs < 5), suggesting a high degree of inter-individual variation in coping with lymphedema. This study showed that BCRL is associated with long-term impairments in HRQoL, especially affecting the physical and psychosocial domains. Surprisingly, BCRL diagnosis rather than clinical severity drove the largest impairments in HRQoL.
When primary care providers and smokers meet: a systematic review and metasynthesis
Authors: Manolios Emilie , Sibeoni Jordan , Teixeira Maria , Révah-Levy Anne , Verneuil Laurence , Jovic Ljiljana 
Published: June 2021

AbstractPrimary Care Providers (PCPs) often deal with patients on daily clinical practice without knowing anything about their smoking status and willingness to quit. The aim of this metasynthesis is to explore the PCPs and patients who are smokers perspectives regarding the issue of smoking cessation within primary care settings. It relies on the model of meta-ethnography and follows thematic synthesis procedures. Twenty-two studies are included, reporting on the view of 580 participants. Three main themes emerge: (i) What lacks, (ii) Some expectations but no request, and (iii) How to address the issue and induce patients’ motivation. Our results reveal a global feeling of a lack of legitimacy among PCPs when it comes to addressing the issue of tobacco and smoking cessation with their patients, even though they have developed creative strategies based on what is at the core of their practice, that is proximity, continuity, long-term and trustworthy relationship.
Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
Authors: Zhang Jian , Fang Xin , Mao Yu , Qi Haochen , Wu Jayne , Liu Xiaoru , You Fangshuo , Zhao Wenci , Chen Ying , Zheng Lei 
Published: June 2021

AbstractDue to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
Advances in 3D peptide hydrogel models in cancer research
Authors: Xu Jingwen , Qi Guangyan , Wang Weiqun , Sun Xiuzhi Susan 
Published: June 2021

AbstractIn vitro cell culture models on monolayer surfaces (2D) have been widely adapted for identification of chemopreventive food compounds and food safety evaluation. However, the low correlation between 2D models and in vivo animal models has always been a concern; this gap is mainly caused by the lack of a three-dimensional (3D) extracellular microenvironment. In 2D models, cell behaviors and functionalities are altered, resulting in varied responses to external conditions (i.e., antioxidants) and hence leading to low predictability. Peptide hydrogel 3D scaffolding technologies, such as PGmatrix for cell culture, have been recently reported to grow organoid-like spheroids physiologically mimicking the 3D microenvironment that can be used as an in vitro 3D model for investigating cell activities, which is anticipated to improve the prediction rate. Thus, this review focuses on advances in 3D peptide hydrogels aiming to introduce 3D cell culture tools as in vitro 3D models for cancer-related research regarding food safety and nutraceuticals.