bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seasonal hydrologic and geologic forcing drive hot spring geochemistry and microbial biodiversity
Authors: Colman Daniel R. , Lindsay Melody R. , Harnish Annette , Bilbrey Evan M. , Amenabar Maximiliano J. , Selensky Matthew J. , Fecteau Kristopher M. , Debes Randall V. , Stott Matthew B. , Shock Everett L. , Boyd Eric S. 
Published: June 2021

SummaryHot springs integrate hydrologic and geologic processes that vary over short‐ and long‐term time scales. However, the influence of temporal hydrologic and geologic change on hot spring biodiversity is unknown. Here, we coordinated near‐weekly, cross‐seasonal (~140 days) geochemical and microbial community analyses of three widely studied hot springs with local precipitation data in Yellowstone National Park. One spring (‘HFS’) exhibited statistically significant, coupled microbial and geochemical variation across seasons that was associated with recent precipitation patterns. Two other spring communities, ‘CP’ and ‘DS’, exhibited minimal to no variation across seasons. Variability in the seasonal response of springs is attributed to differences in the timing and extent of aquifer recharge with oxidized near‐surface water from precipitation. This influx of oxidized water is associated with changes in community composition, and in particular, the abundances of aerobic sulfide‐/sulfur‐oxidizers that can acidify waters. During sampling, a new spring formed after a period of heavy precipitation and its successional dynamics were also influenced by surface water recharge. Collectively, these results indicate that changes in short‐term hydrology associated with precipitation can impact hot spring geochemistry and microbial biodiversity. These results point to potential susceptibility of certain hot springs and their biodiversity to sustained, longer‐term hydrologic changes.
A novel multiplex of 12 multicopy Y‐STRs for forensic application
Authors: Shang Lei , Ding Guangshu , Mo Xiaoting , Sun Jing , Sun Hui , Yu Zhengliang , Li Wanshui 
Published: June 2021

AbstractY chromosomal short tandem repeats (Y‐STRs) have been applied overwhelmingly in forensic areas for solving paternity identification and sexual assault cases. Yet the widely used Y‐STR kits contain mostly single‐copy markers, which may restrict the discrimination power. Here, a novel Y‐STR multiplex was developed and validated in order to complement the currently available Y‐STR kits, especially on differentiating male relatives. The assay includes twelve multicopy Y‐STR loci (DYF371, DYF383S1, DYS385, DYF387S1, DYS389I/II, DYF399S1, DYF404S1, DYF409S1, DYF411S1, DYS464, DYS526, DYS527; four of them are rapidly mutating ones), 1 single‐copy Y‐STR (DYS391), and Amelogenin, and was optimized to amplify at annealing temperature of 59°C and 28 cycles. Validation studies show that full profiles are obtained with 0.125 ng of male DNA. The system is capable of overcoming high concentrations of inhibitors such as hematin, EDTA, and humic acid. Besides, the results demonstrate good sizing precision and the ability to detect male‐specific profiles in male/female DNA mixtures at a ratio of 1:800. Excellent species specificity was also observed in microorganisms and non‐primates, while detectable peaks were found in some primates. Based on published genetic data, gene diversity values were above 0.7 for most of the loci in our multiplex, inferring a high capacity in discriminating unrelated and related male individuals. The kit is of great potential for forensic application.
A phase 3, open‐label, single‐arm study of vadadustat for anemia in chronic kidney disease for Japanese patients on hemodialysis not receiving erythropoiesis‐stimulating agents
Authors: Nangaku Masaomi , Kondo Kazuoki , Takabe Souichirou , Ueta Kiichiro , Tandai Tsubasa , Kawaguchi Yutaka , Komatsu Yasuhiro 
Published: June 2021

AbstractVadadustat is an oral hypoxia‐inducible factor prolyl hydroxylase inhibitor approved in Japan for the treatment of anemia in patients with chronic kidney disease (CKD). This phase 3, open‐label, single‐arm study evaluated the efficacy and safety of vadadustat in 24 Japanese patients with CKD‐associated anemia on hemodialysis who were not receiving erythropoiesis‐stimulating agents (ESAs). Patients received vadadustat for 24 weeks; the starting dose was 300 mg/day and doses were adjusted to achieve the target hemoglobin (Hb) range of 10.0–12.0 g/dL. The least squares mean of average Hb at Weeks 20 and 24 (95% confidence interval) was 10.75 g/dL (10.35, 11.14). The most common adverse event was shunt stenosis (25.0%). Adverse drug reactions (diarrhea and vomiting) occurred in two patients (8.3%) and the severity was mild. Vadadustat increased and maintained Hb levels within the target range and was generally well‐tolerated in Japanese patients with anemia on hemodialysis not receiving ESAs.
Effective doses and associated age‐related risks for common paediatric diagnostic nuclear medicine and PET procedures at a large Australian paediatric hospital
Authors: Earl Victoria J , Baker Luke J , Perdomo Amanda A 
Published: June 2021

AbstractIntroductionEffective dose alone cannot be used to specify and communicate the radiation risk for an individual as risks are dependent on many factors including age and gender. There are limited published data regarding age‐specific effective doses and the associated lifetime risk of developing cancers for paediatrics. In this study, we have estimated the typical effective doses for six commonly performed paediatric nuclear medicine and positron emission tomography (PET) studies at the Royal Children’s Hospital, Melbourne, Australia. Effective doses were used to estimate and categorise associated stochastic risks with commonly used risk terminology.MethodsPaediatric protocols for common nuclear medicine and PET studies and the World Health Organization (WHO) 50th percentile weight‐for‐age data for females and males aged up to 18 years were used to estimate typical organ and effective doses using ICRP dosimetric tables for radiopharmaceuticals and lifetime risk of cancer incidence using BEIR VII Phase 2 report data. Results were used to determine standardised levels of risk.ResultsOrgan doses, effective doses, corresponding lifetime risk of cancer incidence and level of risk category from six common nuclear medicine and PET studies for paediatric patients were calculated and presented for ease of communication.ConclusionTypical effective doses from common paediatric nuclear medicine and PET studies and the associated lifetime risk of cancer incidence and level of risk have been established for our institution. This can be used to convey risks to health professionals, patients and carers in ways that are easy to understand and compare with other everyday risks.
RUBIC (ReproUnion Biobank and Infertility Cohort): A binational clinical foundation to study risk factors, life course, and treatment of infertility and infertility‐related morbidity
Authors: Priskorn Lærke , Tøttenborg Sandra Søgaard , Almstrup Kristian , Andersson Anna‐Maria , Axelsson Jonatan , Bräuner Elvira Vaclavik , Elenkov Angel , Freiesleben Nina la Cour , Giwercman Yvonne Lundberg , Grøndahl Marie Louise , Hansen Ann Holm , Hansen Laura Smidt , Henic Emir , Kitlinski Margareta Laczna , Landersoe Selma Kloeve , Lindh Christian , Løkkegaard Ellen Leth , Malm Johan , Olsen Kristina Wendelboe , Petersen Kajsa Uglevig , Schmidt Lone , Stormlund Sacha , Svendsen Pernille Fog , Vassard Ditte , Wang Nathalie Friis , Zedeler Anne , Bhasin Shalender , Chavarro Jorge , Eisenberg Michael L. , Hauser Russ , Huhtaniemi Ilpo , Krawetz Stephen A. , Marko‐Varga György , Salonia Andrea , Toppari Jorma , Juul Anders , Jørgensen Niels , Nielsen Henriette Svarre , Pinborg Anja , Rylander Lars , Giwercman Aleksander 
Published: June 2021

AbstractBackgroundInfertility affects 15%–25% of all couples during their reproductive life span. It is a significant societal and public health problem with potential psychological, social, and economic consequences. Furthermore, infertility has been linked to adverse long‐term health outcomes. Despite the advanced diagnostic and therapeutic techniques available, approximately 30% of infertile couples do not obtain a live birth after fertility treatment. For these couples, there are no further options to increase their chances of a successful pregnancy and live birth.ObjectivesThree overall questions will be studied: (1) What are the risk factors and natural life courses of infertility, early embryonic loss, and adverse pregnancy outcomes? (2) Can we develop new diagnostic and prognostic biomarkers for fecundity and treatment success? And (3) what are the health characteristics of women and men in infertile couples at the time of fertility treatment and during long‐term follow‐up?Material and MethodsReproUnion Biobank and Infertility Cohort (RUBIC) is established as an add‐on to the routine fertility management at Copenhagen University Hospital Departments in the Capital Region of Denmark and Reproductive Medicine Centre at Skåne University Hospital in Sweden. The aim is to include a total of 5000 couples equally distributed between Denmark and Sweden. The first patients were enrolled in June 2020. All eligible infertile couples are prospectively asked to participate in the project. Participants complete an extensive questionnaire and undergo a physical examination and collection of biospecimens (blood, urine, hair, saliva, rectal swabs, feces, semen, endometrial biopsies, and vaginal swabs). After the cohort is established, the couples will be linked to the Danish and Swedish national registers to obtain information on parental, perinatal, childhood, and adult life histories, including disease and medication history. This will enable us to understand the causes of infertility and identify novel therapeutic options for this important societal problem.
Testicular torsion–detorsion causes dysfunction of mitochondrial oxidative phosphorylation
Authors: Shih Hung‐Jen , Chang Chao‐Yuan , Huang I‐Tao , Tsai Pei‐Shan , Han Chia‐Li , Huang Chun‐Jen 
Published: June 2021

AbstractBackground: Semen quality impairment is a serious consequence of testicular torsion–detorsion. Adequate germ‐cell mitochondrial oxidative phosphorylation plays a crucial role in male fertility. Changes in cellular oxidative phosphorylation in testicular tissues after testicular torsion–detorsion remain unclear.Objectives: This study investigated whether testicular torsion–detorsion induces alternations of mitochondrial oxidative phosphorylation in testicular tissues.Materials and Methods: BALB/c male mice were divided into a Sham group and a testicular torsion–detorsion group. At the end of the procedure, the mice were euthanized, and their bilateral testicles were removed. Mitochondria morphology was evaluated through transmission electron microscopy. The cellular respiratory functions of germ cells were evaluated using a Seahorse analyzer assay. The proteome profiles in testicular tissues were analyzed using liquid chromatography–tandem mass spectrometry. The differences in the expression levels of each component in the oxidative phosphorylation were revealed using Ingenuity Pathways Analysis.Results: Inner mitochondrial membrane disruption was found in ipsilateral twisted testicular mitochondria in the torsion–detorsion group but not in contralateral untwisted testes. The cellular respiratory function in germ cells was significantly decreased after testicular torsion–detorsion in ipsilateral twisted testes but not in contralateral untwisted testes. Liquid chromatography–tandem mass spectrometry analysis of ipsilateral twisted testicular tissue revealed that mitochondrial proteins were differentially expressed after testicular torsion–detorsion. Testicular torsion–detorsion induced downregulation of oxidative phosphorylation and revealed alternations of specific proteins in the oxidative phosphorylation complexes.Discussion and Conclusion: Testicular torsion–detorsion produced mitochondria injury and dysregulation of mitochondrial oxidative phosphorylation in ipsilateral twisted testes. Different protein expressions were identified in the mitochondrial oxidative phosphorylation complexes with testicular torsion–detorsion; new therapeutic targets may be identified to restore the oxidative phosphorylation function of germ cells.
Early repolarization pattern in adult females with eating disorders
Authors: Frederiksen Tanja Charlotte , Krogh Christiansen Morten , Clausen Loa , Kjærulf Jensen Henrik 
Published: June 2021

AbstractIntroductionThe risk of cardiovascular death is increased in patients with eating disorders (ED), but the background for this is unknown. Early repolarization pattern (ERP) on the electrocardiogram (ECG) has been associated with increased risk of sudden cardiac death.MethodsWe investigated the prevalence of ERP in 233 female patients with anorexia nervosa (AN) and bulimia nervosa (BN) (age 18–35 years) compared with 123 healthy female controls.ResultsEarly repolarization pattern was present in 52 (22%) of ED patients (16 (15%) AN patients and 36 (29%) BN patients) and 17 (14%) of healthy controls. When adjusting for age, BMI, heart rate, use of selective serotonin reuptake inhibitors (SSRI), and potassium level, the odds ratio (OR) for ERP was 2.1 (95% CI 1.1–4.2, p = .03). There was an increased prevalence of inferolateral ERP in patients with ED compared with healthy controls (OR = 4.3, 95% CI 1.7–11.3, p = .003) as well as ERP with a downward/horizontal sloping ST segment (OR = 3.1, 95% CI 1.3–7.6, p = .01). Additionally, J‐point elevation >0.2 mV was more prevalent in patients with ED (OR = 3.3, 95% CI 1.1–9.7, p = .03).ConclusionThe prevalence of ERP was increased in patients with ED compared with healthy controls. This finding may provide a possible explanation for the increased cardiovascular mortality in ED patients.
Comparison of viscocanalostomy plus suture‐assisted near‐360‐degree trabeculotomy and viscocanalostomy plus rigid probe trabeculotomy in primary congenital glaucoma
Authors: Yu Qiuli , Liang Ya , Ji Fangfang , Sun Hong , Zhang Xiaojun , Zhou Qing , Chen Zhijun , Gu Hongwei , Yuan Zhilan 
Published: June 2021

AbstractPurposeTo compare the efficacy and safety of viscocanalostomy plus near‐360‐degree suture trabeculotomy (VST) with viscocanalostomy plus rigid probe trabeculotomy (VT) in treating primary congenital glaucoma (PCG) over a one‐year follow‐up.MethodsThis consecutive retrospective study included patients with PCG confirmed within 3 years of age from March 2017 to October 2019. Efficacy was evaluated by comparing the postoperative intraocular pressure (IOP) curve and the success rate at one year after surgery. Safety was assessed by comparing the postoperative complications. The number of anti‐glaucoma agents, horizontal corneal diameter (HCD) and cup‐to‐disc ratio (C/D) of the two surgical methods were also compared.ResultsData of 90 eyes from 61 patients were analysed. The baseline parameters of the two groups were similar. The IOP at 12 months after surgery in the VST group was 12.7 ± 4.8 mmHg, while that in the VT group was 15.8 ± 6.5 mmHg. The IOP at 6, 9 and 12 months postoperatively in the VST group was significantly lower than in the VT group (p < 0.05). Viscocanalostomy plus near‐360‐degree suture trabeculotomy (VST) remained a significant favourable factor for complete one‐year success (93.6% versus 74.4%, p = 0.005) but not qualified one‐year success (97.9% versus 88.4%, p = 0.06). The number of anti‐glaucoma agents, HCD and C/D were reduced in both groups. Postoperative complications were not significantly different between the two groups.ConclusionsIn children with PCG, VST provides a more durable IOP control than VT over the one‐year follow‐up, with a similar safety profile.
Kidney transplantation from expanded criteria donors: an increased risk of urinary complications – the UriNary Complications Of Renal Transplant (UNyCORT) study
Authors: Mesnard Benoit , Leroy Maxime , Hunter James , Kervella Delphine , Timsit Marc‐Olivier , Badet Lionel , Glemain Pascal , Morelon Emmanuel , Buron Fanny , Le Quintrec‐Donnette Moglie , Pernin Vincent , Ladriere Marc , Girerd Sophie , Legendre Christophe , Sicard Antoine , Albano Laeticia , De Vergie Stephane , Kerleau Clarisse , Prudhomme Thomas , Rigaud Jérôme , Cantarovich Diego , Blancho Gilles , Karam Georges , Giral Magali , Ville Simon , Branchereau Julien ,   For the Données Informatisées et VAlidées en Transplantation/Computerized and VAlidated Data in Transplantation (DIVAT) Consortium Affiliations
Published: June 2021

ObjectivesTo assess the impact of expanded criteria donors (ECD) on urinary complications in kidney transplantation.Patients and MethodsThe UriNary Complications Of Renal Transplant (UNyCORT) is a cohort study based on the French prospective Données Informatisées et VAlidées en Transplantation/Computerized and VAlidated Data in Transplantation (DIVAT) cohort. Data were extracted between 1 January 2002 and 1 January 2018 with 1‐year minimum follow‐up, in relation to 44 pre‐ and postoperative variables. ECD status was included according to United Network for Organ Sharing (UNOS) definition. The primary outcome of the UNyCORT study was the association between the donor’s ECD/standard criteria donors (SCD) status and urinary complications at 1 year in uni‐ and multivariate analysis. Sub‐group analysis, stratified analysis on ECD/SCD donor’s status and transplant failure analysis were then conducted.ResultsBetween 1 January 2002 and 1 January 2018, 10 279 kidney transplants in adult recipients were recorded within the DIVAT network. A total of 8559 (83.4%) donors were deceased donors and 1699 (16.6%) were living donors (LD). Among donation after circulatory death (DCD) donors, 224 (2.85%) were uncontrolled DCD and 93 (1.09%) were controlled DCD donors. A total of 3617 (43.9%) deceased donors were ECD. The overall urological complication rate was 16.26%. The donor’s ECD status was significantly associated with an increased risk of urological complications at 1 year in multivariate analysis (odds ratio: 1.50, 95% CI 1.31–1.71; P < 0.001) and especially with stenosis and ureteric fistulae at 1 year. There is no association with LD, uncontrolled and controlled DCD. The placement of an endo‐ureteric stent was beneficial in preventing urinary complications in all donors and particularly in ECD donors.ConclusionThe donor’s ECD status is associated with a higher likelihood of stenosis and ureteric fistulae at 1 year. Recipients of grafts from ECD donors should probably be considered for closer urological monitoring and systematic preventive measures.
Identification of novel autoantibody signatures and evaluation of a panel of autoantibodies in breast cancer
Authors: Qiu Cuipeng , Wang Bofei , Wang Peng , Wang Xiao , Ma Yan , Dai Liping , Shi Jianxiang , Wang Keyan , Sun Guiying , Ye Hua , Zhang Jianying 
Published: June 2021

AbstractTumor‐associated autoantibodies (TAAb) could be serological tumor markers. This study aims to discover novel TAAb signatures for breast cancer (BC) detection. The protein microarray was used to identify candidate TAAb, which were further validated in 1197 sera from BC, benign breast diseases (BD), and healthy controls (HC) by enzyme‐linked immunosorbent assay. In addition, 319 preoperative and postoperative sera were evaluated. A panel was determined using four different classifiers. Twelve TAAb were identified with frequencies of 15.8%‐59.2%; their levels were significantly decreased in postoperative sera compared to those in preoperative sera (P < .05). A panel with six TAAb was developed and evaluated. The area under the curve (AUC) was 0.879 (74.3% sensitivity, 91.9% specificity) and 0.865 (69.7% sensitivity, 91.7% specificity) for distinguishing BC from HC in the training set and test set, respectively. The panel had an AUC of .884 (71.2% sensitivity, 90.5% specificity) for discriminating BC from BD. For identifying BC from all controls (HC+BD), the AUC was .916 (78.9% sensitivity, 90.2% specificity). The AUC of the panel was .920 and .934 for distinguishing stage I‐II and age < 50 BC from HC, respectively. These identified TAAb have the potential to provide a non–invasive approach to detect BC.
Ecological corridors in Costa Rica: An evaluation applying landscape structure, fragmentation‐connectivity process, and climate adaptation
Authors: Beita Carlos Morera , Murillo Luis Fernando Sandoval , Alvarado Luis Diego Alfaro 
Published: June 2021

AbstractIn recent years, ecological corridors have been proposed on a global scale as a response to the accelerated process of natural ecosystem fragmentation, mainly as a result of human impact. In accordance with this trend, Costa Rica has undergone a process of implementing ecological corridors as to promote ecological connectivity since the 1990s, with the establishment of 44 ecological corridors covering 38% of Costa Rica's territory. Nevertheless, there is no research evaluating these corridors on a national scale that takes into account their functions as conduits, barriers, and habitats. Thus, the objective of this research was to describe the process of biological corridor formation in Costa Rica, and to evaluate the potential effectiveness of corridors by considering aspects of landscape structure and ecological processes related to connectivity and fragmentation. We used the National Program of Ecological Corridors database along with coverage analysis from Landsat images from 2000 and 2015.The composition of the biological corridors was determined at the landscape scale and related to potential to maintain a specific population of wild mammals weighing more than 10 kg. The composition of the ecological corridors was highly variable in terms of total area, proportion of natural habitat, and fragmentation process. Most biological corridors are capable of maintaining viable populations of Pecari tajacu and Tapir bairdii, while none could maintain populations of Panthera onca and Tayassu pecari. Only 50% of the biological corridors had improved in their connectivity. Therefore, public policies, such as master plans focusing on ecosystem restoration must be established. In addition, only two biological corridors incorporate the majority of elevation ranges (Life Zones) present in the country, which reduces the potential of the corridor system as a tool for climate change adaptation.