bluuebunny/crossref_metadata_2025_split huggingface dataset:
HCP5, as the sponge of miR-1291, facilitates AML cell proliferation and restrains apoptosis via increasing PIK3R5 expression
Authors: Liu Yan , Jing Xue-Bing , Wang Zhen-Cheng , Han Qing-Kun 
Published: June 2021

Abstract
Background
Acute myeloid leukemia (AML) is recognized as a hematological neoplasm with heterogenetic cytology and short-term outcome. HCP5 has been proven to be related with the pathogenesis of AML. However, the underlying mechanism of HCP5 in AML remains unclear.

Methods
Clinical profiles of AML patients were downloaded from TCGA and GTEx databases. LncBase and TargetScan online tools were utilized to predict potential targets, and dual-luciferase reporter assay was performed to verify the association between miR-1291 and HCP5 or PIK3R5. Cell Counting Kit 8 and flow cytometry tests were implemented to evaluate the effects of HCP5/miR-1291/PIK3R5 axis in AML cells. Quantitative RT-PCR and Western blot were conducted to detect the expression levels of genes.

Results
HCP5 and PIK3R5 were significantly increased in AML tissue samples compared with healthy controls. HCP5 facilitated AML cells viability and inhibited apoptosis. There was a positive relationship between HCP5 and PIK3R5, but miR-1291 negatively regulated PIK3R5. Overexpression of PIK3R5 enhanced the promoting effect of HCP5 in the development of AML, while weakened the suppression of miR-1291 to AML progression.

Conclusion
Our findings manifested that HCP5 was remarkably upregulated in AML and upregulated HCP5 promoted the malignant behaviors of AML cells by mediating miR-1291/PIK3R5 axis, which would provide a new insight for the treatment of AML.
Identification of hub genes associated with prognosis, diagnosis, immune infiltration and therapeutic drug in liver cancer by integrated analysis
Authors: Lei Xinyi , Zhang Miao , Guan Bingsheng , Chen Qiang , Dong Zhiyong , Wang Cunchuan 
Published: June 2021

Abstract
Background
Liver cancer is one of the most common cancers and causes of cancer death worldwide. The objective was to elucidate novel hub genes which were benefit for diagnosis, prognosis, and targeted therapy in liver cancer via integrated analysis.

Methods
GSE84402, GSE101685, and GSE112791 were filtered from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified by using the GEO2R. The GO and KEGG pathway of DEGs were analyzed in the DAVID. PPI and TF network of the DEGs were constructed by using the STRING, TRANSFAC, and Harmonizome. The relationship between hub genes and prognoses in liver cancer was analyzed in UALCAN based on The Cancer Genome Atlas (TCGA). The diagnostic value of hub genes was evaluated by ROC. The relationship between hub genes and tumor-infiltrate lymphocytes was analyzed in TIMER. The protein levels of hub genes were verified in HPA. The interaction between the hub genes and the drug were identified in DGIdb.

Results
In total, 108 upregulated and 60 downregulated DEGs were enriched in 148 GO terms and 20 KEGG pathways. The mRNA levels and protein levels of CDK1, HMMR, PTTG1, and TTK were higher in liver cancer tissues compared to normal tissues, which showed excellent diagnostic and prognostic value. CDK1, HMMR, PTTG1, and TTK were positively correlated with tumor-infiltrate lymphocytes, which might involve tumor immune response. The CDK1, HMMR, and TTK had close interaction with anticancer agents.

Conclusions
The CDK1, HMMR, PTTG1, and TTK were hub genes in liver cancer; hence, they might be potential biomarkers for diagnosis, prognosis, and targeted therapy of liver cancer.
Access to quality diagnosis and rational treatment for tuberculosis: real-world evidence from China–Gates Tuberculosis Control Project Phase III
Authors: Li Zhi-Peng , Mao Wen-Hui , Huang Fei , Wang Ni , Ma Li-Ping , Zhang Li-Qun , Gao Meng-Qiu , Wang Wei-Bing , Zhao Qi , Tang Sheng-Lan 
Published: June 2021

Abstract
Background
China has successfully reduced tuberculosis (TB) incidence rate over the past three decades, however, challenges remain in improving the quality of TB diagnosis and treatment. In this paper, we assess the effects of the implementation of “China National Health Commission (NHC) and Gates Foundation TB Prevention and Control Project” on the quality of TB care in the three provinces.

Methods
We conducted the baseline study in 2016 and the final evaluations in 2019 in the 12 selected project counties. We obtained TB patients’ information from the TB Information Management System and reviewed medical records of TB cases in the TB designated hospitals. We compared TB diagnosis and treatment services with the national practice guideline and used Student’s t-test and Pearson χ2 tests or Fisher’s exact tests to compare the difference before and after the project implementation.

Results
The percentage of sputum smear-negative (SS–) patients taking culture or rapid molecular test (RMT) doubled between 2015 and 2018 (from 35% to 87%), and the percentage of bacteriologically confirmed pulmonary TB cases increased from 36% to 52%. RMT has been widely used and contributed an additional 20% of bacteriologically confirmed TB cases in 2018. The percentage of TB patients taking drug susceptibility tests (DST) also doubled (from 40% in 2015 to 82% in 2018), and the proportion of TB patients receiving adequate diagnosis services increased from 85% to 96%. Among all SS– TB patients, over 86% received the recommended diagnostic services at the end of the study period, an improvement from 75% prior to the project implementation. However, the proportion of TB patients treated irrationally using second-line anti-TB drugs (SLDs) increased from 12.6% in 2015 to 19.9% in 2018. The regional disparities remained within the project provinces, albeit the gaps between them narrowed down for almost all indicators.

Conclusions
The quality of TB diagnosis services has been improved substantially, which is attributable to the coverage of new diagnosis technology. However, irrational use of SLDs remains a concern after the project implementation.

Graphic abstract
Severe fever with thrombocytopenia syndrome with re-infection in China: a case report
Authors: Lv Shou-Ming , Yuan Chun , Zhang Lan , Wang Yu-Na , Dai Zi-Niu , Yang Tong , Dai Ke , Zhang Xiao-Ai , Lu Qing-Bin , Yang Zhen-Dong , Cui Ning , Li Hao , Liu Wei 
Published: June 2021

Abstract
Background
Severe fever with thrombocytopenia syndrome (SFTS), an emerging tickborne infectious disease caused by a novel banyangvirus (SFTS virus, SFTSV), was endemic in several Asian countries with a high mortality up to 30%. Until recently, SFTSV-associated re-infection have not been reported and investigated.

Case presentation
A 42-year-old female patient was identified as a case of SFTS with re-infection, with two episodes of SFTSV infection on June 2018 and May 2020. The diagnosis of SFTS was confirmed by detection of SFTSV RNA in the blood samples using real-time reverse-transcription polymerase chain reaction and antibodies specific for SFTSV using enzyme linked immunosorbent assay. The changes of viremia and antibody response differed between the two episodes. Phylogenetic analysis showed the two viral genome sequences were in the same clade, but showing 0.6% dissimilarity of the nearly whole nucleotide sequence. Analysis of clinical data revealed that the second episode showed milder illness than that of the first episode.

Conclusions
Epidemiological and clinical findings, viral whole genomic sequences, and serological evidence, provided evidence for the re-infection of SFTSV rather than prolonged viral shedding or relapse of the original infection. The patients with re-infection of SFTSV may be at high odds of clinically inapparent or mildly symptomatic. More attention should be directed towards the long-term follow up of the recovered patients in the future, to explicitly acquire the decay profile of their immunity response.

Graphic abstract
Understanding implicit and explicit learning in adolescents with and without anorexia nervosa
Authors: Sternheim Lot C. , Wickham Miriam I. , Danner Unna N. , Maddox Todd W. , Filoteo Vincent J. , Shott Megan E. , Frank Guido K. W. 
Published: June 2021

Abstract
Background
Cognitive disturbances such as impairments in learning are thought to play a role in adult Anorexia Nervosa (AN). It is remains unclear to what extent these disturbances result from starvation of the brain, or relate to an abnormal premorbid cognitive profile. This study investigates learning processes in adolescents with AN, hypothesizing that implicit learning is intact, as found previously in explicit learning tasks. Secondly, we hypothesized that anxiety and depression symptoms, inherent to AN, are associated to learning processes in AN.

Methods
In total 46 adolescents diagnosed with AN and 44 control participants were administered an implicit category learning task in which they were asked to categorize simple perceptual stimuli (Gabor patches) based on a linear integration (i.e., an implicit task) of orientation and spatial frequency of the stimulus. A subgroup of adolescents (n = 38) also completed a task assessing explicit learning.

Results
Model-based analyses indicated that adolescents with AN performed significantly more accurately compared to their healthy peers regardless of whether they used the optimal strategy or not. Depression and anxiety did not relate to learning performance in the AN group.

Conclusions
Overall, our findings of augmented implicit and explicit learning in adolescents with AN corroborate recent studies that suggested higher stimulus-response learning during prediction error paradigms. Learning disturbances in adult AN may then be at least partly due to long-term malnourishment, highlighting the importance of early recognition and refeeding in treatments for AN.
Risk and protective factors for the development of gambling-related harms and problems among Australian sexual minority men
Authors: Bush Rachel , Russell Alex M. T. , Staiger Petra K. , Waling Andrea , Dowling Nicki A. 
Published: June 2021

AbstractBackgroundSexual minority men (SMM) often experience stressful social environments dominated by stigma and discrimination. SMM are typically more likely than heterosexual men to engage in certain risky behaviours such as problem gambling. This study aimed to compare gambling behaviour among SMM and examine potential risk factors (erroneous gambling cognitions, gambling outcome expectancies, hazardous alcohol use, impulsivity, and psychological distress; as well as perceived stigma and discrimination for the SMM participants) and potential protective factors (resilience, social support, and community connectedness) for problem gambling severity and gambling-related harms among SMM living in Australia.MethodsAn online survey, with an over-representation of SMM participants and problem, moderate-risk, and low-risk gamblers, was completed by 101 SMM (mean age = 28.5) and 207 heterosexual men (mean age = 26.4).ResultsSMM were found to have significantly lower levels of problem gambling severity compared with heterosexual men, and report significantly lower gambling participation, frequencies and expenditure on any gambling activity, casino table games, horse racing/greyhound betting, sports betting, and keno. However, in the SMM group, 38.3% were classified in the problem gambling category of the Problem Gambling Severity Index and 27.6% were classified in the moderate-risk gambling category. There were no significant differences between groups in gambling-related harms. Multiple regression analyses revealed that problem gambling severity and related harms were independently predicted by higher levels of impulsivity and erroneous gambling cognitions for both groups.ConclusionsLower frequency of gambling behaviours among SMM and similar risk factors predicting problem gambling severity/harms for both groups suggests that problem gambling is not pronounced among SMM. This study adds new evidence to the gambling literature which can be used as comparative benchmarks for future research.
The spatialization of time and history in the skyscrapers of the twenty-first century in Shanghai
Authors: Roche Cárcel Juan Antonio 
Published: June 2021

AbstractThis article aims to find out to what extent the skyscrapers erected in the late twentieth and early twenty-first centuries, in Shanghai, follow the modern program promoted by the State and the city and how they play an essential role in the construction of the temporary discourse that this modernization entails. In this sense, it describes how the city seeks modernization and in what concrete way it designs a modern temporal discourse. The work finds out what type of temporal narrative expresses the concentration of these skyscrapers on the two banks of the Huangpu, that of the Bund and that of the Pudong, and finally, it analyzes the seven most representative and significant skyscrapers built in the city in recent years, in order to reveal whether they opt for tradition or modernity, globalization or the local. The work concludes that the past, present and future of Shanghai have been minimized, that its history has been shortened, that it is a liminal site, as its most outstanding skyscrapers, built on the edge of the river and on the border between past and future. For this reason, the author defends that Shanghai, by defining globalization, by being among the most active cities in the construction of skyscrapers, by building more than New York and by building increasingly technologically advanced tall towers, has the possibility to devise a peculiar Chinese modernity, or even deconstruct or give a substantial boost to the general concept of Western modernity.
Mouse closed head traumatic brain injury replicates the histological tau pathology pattern of human disease: characterization of a novel model and systematic review of the literature
Authors: Kahriman Aydan , Bouley James , Smith Thomas W. , Bosco Daryl A. , Woerman Amanda L. , Henninger Nils 
Published: June 2021

AbstractTraumatic brain injury (TBI) constitutes one of the strongest environmental risk factors for several progressive neurodegenerative disorders of cognitive impairment and dementia that are characterized by the pathological accumulation of hyperphosphorylated tau (p-Tau). It has been questioned whether mouse closed-head TBI models can replicate human TBI-associated tauopathy. We conducted longitudinal histopathological characterization of a mouse closed head TBI model, with a focus on pathological features reported in human TBI-associated tauopathy. Male C57BL/6 J mice were subjected to once daily TBI for 5 consecutive days using a weight drop paradigm. Histological analyses (AT8, TDP-43, pTDP-43, NeuN, GFAP, Iba-1, MBP, SMI-312, Prussian blue, IgG, βAPP, alpha-synuclein) were conducted at 1 week, 4 weeks, and 24 weeks after rTBI and compared to sham operated controls. We conducted a systematic review of the literature for mouse models of closed-head injury focusing on studies referencing tau protein assessment. At 1-week post rTBI, p-Tau accumulation was restricted to the corpus callosum and perivascular spaces adjacent to the superior longitudinal fissure. Progressive p-Tau accumulation was observed in the superficial layers of the cerebral cortex, as well as in mammillary bodies and cortical perivascular, subpial, and periventricular locations at 4 to 24 weeks after rTBI. Associated cortical histopathologies included microvascular injury, neuroaxonal rarefaction, astroglial and microglial activation, and cytoplasmatic localization of TDP-43 and pTDP-43. In our systematic review, less than 1% of mouse studies (25/3756) reported p-Tau using immunostaining, of which only 3 (0.08%) reported perivascular p-Tau, which is considered a defining feature of chronic traumatic encephalopathy. Commonly reported associated pathologies included neuronal loss (23%), axonal loss (43%), microglial activation and astrogliosis (50%, each), and beta amyloid deposition (29%). Our novel model, supported by systematic review of the literature, indicates progressive tau pathology after closed head murine TBI, highlighting the suitability of mouse models to replicate pertinent human histopathology.
A graph-based big data optimization approach using hidden Markov model and constraint satisfaction problem
Authors: Sassi Imad , Anter Samir , Bekkhoucha Abdelkrim 
Published: June 2021

AbstractTo address the challenges of big data analytics, several works have focused on big data optimization using metaheuristics. The constraint satisfaction problem (CSP) is a fundamental concept of metaheuristics that has shown great efficiency in several fields. Hidden Markov models (HMMs) are powerful machine learning algorithms that are applied especially frequently in time series analysis. However, one issue in forecasting time series using HMMs is how to reduce the search space (state and observation space). To address this issue, we propose a graph-based big data optimization approach using a CSP to enhance the results of learning and prediction tasks of HMMs. This approach takes full advantage of both HMMs, with the richness of their algorithms, and CSPs, with their many powerful and efficient solver algorithms. To verify the validity of the model, the proposed approach is evaluated on real-world data using the mean absolute percentage error (MAPE) and other metrics as measures of the prediction accuracy. The conducted experiments show that the proposed model outperforms the conventional model. It reduces the MAPE by 0.71% and offers a particularly good trade-off between computational costs and the quality of results for large datasets. It is also competitive with benchmark models in terms of the running time and prediction accuracy. Further comparisons substantiate these experimental findings.
Parametric study of glycerol and contaminants removal from biodiesel through solvent-aided crystallization
Authors: Ahmad Mohd. Afnan , Letchumanan Arun , Samsuri Shafirah , Mazli Wan Nur Athirah , Md Saad Juniza 
Published: June 2021

AbstractAt present, biodiesel is known as an alternative fuel globally. It is also known that the purification of biodiesel before consumption is mandatory to comply with international standards. Commonly, purification using water washing generates a massive amount of wastewater with a high content of organic compounds that can harm the environment. Therefore, this study applied and tested a waterless method, i.e., the solvent-aided crystallization (SAC), to remove glycerol and other traces of impurities in the crude biodiesel. The parameters of coolant temperature, crystallization time, and stirring rate on the SAC system were investigated. It was discovered that with 14 °C coolant temperature, 300 RPM and higher cooling time result in the highest percentage of FAME up to 99.54%, which indicates that contaminants' presence is limited in the purified biodiesel. The use of 1-butanol as the solvent for crystallization process remarkably enhanced the separation and improved the higher biodiesel quality.
Prediction of buckling force in hourglass-shaped specimens
Authors: Gjengedal Ragnar , Fyllingen Ørjan , Heinik Vojtech 
Published: June 2021

AbstractIt is important to avoid buckling during low-cycle fatigue testing. The buckling load is dependent on the specimen shape, material properties, and the testing machine. In the present investigation of hourglass-shaped specimens the importance of the diameter to radius of curvature is examined. Diameters of 5 and 7 mm are examined with a ratio of radius of curvature to diameter of 4, 6, and 8. The machine used is an Instron 8800 with elongated rods for a climate chamber. This leads to a reduced stiffness of the machine during compression testing. A finite element model (in Abaqus) is developed to identify the critical buckling force. For hourglass-shaped specimens, buckling means onset of sideways movement, without a drop in the applied load which is typical for conventional Euler buckling. The onset of sideways movement is identified experimentally by analysis of the data from extensometer and the load cell. This model is verified by experiments and fits within 0.6 to − 11% depending on the specimen diameter and diameter to radius of curvature ratio. The smallest deviations are obtained for the 7-mm-diameter specimen with deviation varying from 0.6 to − 3.3% between the model and the experiments. The current investigation is done with a commercially available hot rolled structural steel bar of Ø16 mm.
Cap polyposis treated with laparoscopic-assisted total proctocolectomy and ileal J-pouch anal anastomosis: a case report
Authors: Minagawa Tomohiro , Ikeuchi Hiroki , Kusunoki Kurando , Kuwahara Ryuichi , Horio Yuki , Kihara Takako , Hirota Seiichi , Uchino Motoi 
Published: June 2021

Abstract
Background
Cap polyposis (CP) is extremely rare in Japan, and there is no established cure. We report a case in which CP was improved by surgical treatment.

Case presentation
A 48-year-old man was investigated at a local hospital because of diarrhea and bloody stools in 2018. The patient was treated with metronidazole for suspected amoebic dysentery, but his symptoms did not improve. Subsequent close examination revealed possible CP, but treatment with 5-aminosalicylic acid and a steroid enema had no effect. The patient was then referred to our hospital. The bloody stools, diarrhea, and abdominal pain worsened despite medical treatment, so laparoscopic-assisted total proctocolectomy and ileal J-pouch anal anastomosis with ileostomy were performed. CP has no known cause or established treatment, but Helicobacter pylori (HP) infection has been reported in many CP cases in Japan, and HP eradication is often successful. This patient was HP-negative and did not improve with antimicrobial treatment, but the symptoms improved after surgery.

Conclusions
Even after surgery, CP recurrence reportedly occurs within a short period in many cases. However, our patient has had no signs of CP recurrence during 1 year of follow-up.