bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding airspace in leaves: 3D anatomy and directional tortuosity
Authors: Harwood Richard , Théroux‐Rancourt Guillaume , Barbour Margaret M 
Published: May 2021

AbstractThe leaf intercellular airspace is a tortuous environment consisting of cells of different shapes, packing densities, and orientation, all of which have an effect on the travelling distance of molecules from the stomata to the mesophyll cell surfaces. Tortuosity, the increase in displacement over the actual distance between two points, is typically defined as encompassing the whole leaf airspace, but heterogeneity in pore dimensions and orientation between the spongy and palisade mesophyll likely result in heterogeneity in tortuosity along different axes and would predict longer traveling distance along the path of least tortuosity, such as vertically within the columnar cell matrix of the palisade layer. Here, we compare a previously established geometric method to a random walk approach, novel for this analysis in plant leaves, in four different Eucalyptus species. The random walk method allowed us to quantify directional tortuosity across the whole leaf profile, and separately for the spongy and palisade mesophyll. For all species tortuosity was higher in the palisade mesophyll than the spongy mesophyll and horizontal (parallel to the epidermis) tortuosity was consistently higher than vertical (from epidermis to epidermis) tortuosity. We demonstrate that a random walk approach improves on previous geometric approaches and is valuable for investigating CO2 and H2O transport within leaves.
COVID‐19 vaccine hesitancy among women leaving jails: A qualitative study
Authors: Geana Mugur V. , Anderson Sherri , Ramaswamy Megha 
Published: May 2021

AbstractIn many correctional facilities across the United States, COVID‐19 vaccine refusal rates are as high as 50%. Most women leaving jails have low SES, health literacy, and mistrust of governmental institutions, thus exacerbating existing health disparities and making women leaving jail vulnerable. Data from 25 interviews with recently released women suggest that interventions to promote vaccines to this population will have to address health education and mitigate mistrust, misinformation, and conspiracy theories.
Transcriptome of Cenostigma pyramidale roots, a woody legume, under different salt stress times
Authors: Frosi Gabriella , Ferreira‐Neto José Ribamar Costa , Bezerra‐Neto João Pacífico , Pandolfi Valesca , da Silva Manassés Daniel , de Lima Morais David Anderson , Benko‐Iseppon Ana Maria , Santos Mauro Guida 
Published: May 2021

AbstractSalinity stress has a significant impact on the gain of plant biomass. Our study provides the first root transcriptome of Cenostigma pyramidale, a tolerant woody legume from a tropical dry forest, under three different salt stress times (30 min, 2 h, and 11 days). The transcriptome was assembled using the RNA sequencing (RNA‐Seq) de novo pipeline from GenPipes. We observed 932, 804, and 3157 upregulated differentially expressed genes (DEGs) and 164, 273, and 1332 downregulated DEGs for salt over 30 min, 2 h, and 11 days, respectively. For DEGs annotated with the Viridiplantae clade in the early stress periods, the response to salt stress was mainly achieved by stabilizing homeostasis of such ions like Na+ and K+, signaling by Ca2+, transcription factor modulation, water transport, and oxidative stress. For salt stress at 11 days, we observed a higher modulation of transcription factors including the WRKY, MYB, bHLH, NAC, HSF, and AP2‐EREBP families, as well as DEGs involved in hormonal responses, water transport, sugar metabolism, proline, and reactive oxygen scavenging mechanisms. Five selected DEGs (K+ transporter, aquaporin, glutathione S‐transferase, cyclic nucleotide‐gated channel, and superoxide dismutase) were validated by qPCR. Our results indicated that C. pyramidale had an early perception of salt stress modulating ionic channels and transporters, and as the stress progressed, the focus turned to the antioxidant system, aquaporins, and complex hormone responses. The results of this first root transcriptome provide clues on how this native species modulate gene expression to achieve salt stress tolerance.
An Arabidopsis expression predictor enables inference of transcriptional regulators for gene modules
Authors: Geng Haiying , Wang Meng , Gong Jiazhen , Xu Yupu , Ma Shisong 
Published: May 2021

SummaryThe regulation of gene expression by transcription factors (TFs) has been studied for a long time, but no model that can accurately predict transcriptome profiles based on TF activities currently exists. Here, we developed a computational approach, named EXPLICIT (Expression Prediction via Log‐linear Combination of Transcription Factors), to construct a universal predictor for Arabidopsis to predict the expression of 29 182 non‐TF genes using 1678 TFs. When applied to RNA‐Seq samples from diverse tissues, EXPLICIT generated accurate predicted transcriptomes correlating well with actual expression, with an average correlation coefficient of 0.986. After recapitulating the quantitative relationships between TFs and their target genes, EXPLICIT enabled downstream inference of TF regulators for genes and gene modules functioning in diverse plant pathways, including those involved in suberin, flavonoid, glucosinolate metabolism, lateral root, xylem, secondary cell wall development or endoplasmic reticulum stress response. Our approach showed a better ability to recover the correct TF regulators when compared with existing plant tools, and provides an innovative way to study transcriptional regulation.
PAL‐mediated SA biosynthesis pathway contributes to nematode resistance in wheat
Authors: Zhang Haili , Huang Qiulan , Yi Ling , Song Xiaona , Li Lin , Deng Guangbing , Liang Junjun , Chen Fang , Yu Maoqun , Long Hai 
Published: May 2021

SummaryThe pathogen cereal cyst nematode (CCN) is deleterious to Triticeae crops and is a threat to the global crop yield. Accession no. 1 of Aegilops variabilis, a relative of Triticum aestivum (bread wheat), is highly resistant to CCN. Our previous study demonstrated that the expression of the phenylalanine ammonia lyase (PAL) gene AevPAL1 in Ae. variabilis is strongly induced by CCN. PAL, the first enzyme of phenylpropanoid metabolism, is involved in abiotic and biotic stress responses. However, its role in plant–CCN interaction remains unknown. In the present study, we proved that AevPAL1 helps to confer CCN resistance through affecting the synthesis of salicylic acid (SA) and downstream secondary metabolites. The silencing of AevPAL1 increased the incidence of CCN infection in roots and decreased the accumulation of SA and phenylalanine (Phe)‐derived specialized metabolites. The exogenous pre‐application of SA also improved CCN resistance. Additionally, the functions of PAL in phenylpropanoid metabolism correlated with tryptophan decarboxylase (TDC) functioning in tryptophan metabolism pathways. The silencing of either AevPAL1 or AevTDC1 exhibited a concomitant reduction in the expression of both genes and the contents of metabolites downstream of PAL and TDC. These results suggested that AevPAL1, possibly in coordination with AevTDC1, positively contributes to CCN resistance by altering the downstream secondary metabolites and SA content in Ae. variabilis. Moreover, AevPAL1 overexpression significantly enhanced CCN resistance in bread wheat and did not exhibit significant negative effects on yield‐related traits, suggesting that AevPAL1 is valuable for the genetic improvement of CCN resistance in bread wheat.
Mitochondrial ATP synthase subunit d, a component of the peripheral stalk, is essential for growth and heat stress tolerance in Arabidopsis thaliana
Authors: Liu Tianxiang , Arsenault Jesse , Vierling Elizabeth , Kim Minsoo 
Published: May 2021

SummaryAs rapid changes in climate threaten global crop yields, an understanding of plant heat stress tolerance is increasingly relevant. Heat stress tolerance involves the coordinated action of many cellular processes and is particularly energy demanding. We acquired a knockout mutant and generated knockdown lines in Arabidopsis thaliana of the d subunit of mitochondrial ATP synthase (gene name: ATPQ, AT3G52300, referred to hereafter as ATPd), a subunit of the peripheral stalk, and used these to investigate the phenotypic significance of this subunit in normal growth and heat stress tolerance. Homozygous knockout mutants for ATPd could not be obtained due to gametophytic defects, while heterozygotes possess no visible phenotype. Therefore, we used RNA interference to create knockdown plant lines for further studies. Proteomic analysis and blue native gels revealed that ATPd downregulation impairs only subunits of the mitochondrial ATP synthase (complex V). Knockdown plants were more sensitive to heat stress, had abnormal leaf morphology, and were severely slow growing compared to wild type. These results indicate that ATPd plays a crucial role in proper function of the mitochondrial ATP synthase holoenzyme, which, when reduced, leads to wide‐ranging defects in energy‐demanding cellular processes. In knockdown plants, more hydrogen peroxide accumulated and mitochondrial dysfunction stimulon (MDS) genes were activated. These data establish the essential structural role of ATPd and support the importance of complex V in normal plant growth, and provide new information about its requirement for heat stress tolerance.
The unequal geography of declining young adult homeownership: Divides across age, class, and space
Authors: Hochstenbach Cody , Arundel Rowan 
Published: May 2021

AbstractAcross countries, the position of young adults on the housing market has worsened over the past decade. Young adults’ decreasing access to homeownership has garnered particular attention. Most studies analysing young adults’ housing market entry focus on micro‐level determinants or national‐level patterns and trends. This paper adds an important perspective by focusing on spatial variations within a single country, unravelling pronounced inter‐municipal differences in homeownership across age and class. Our case is the Netherlands, where we use full‐population register data for the 2011–2018 period to analyse spatial patterns and trends using a range of quantitative spatial methods. Our findings highlight a notable decrease in owner‐occupancy among young adults in the Netherlands over the period. Such overall declines, however, belie clear class divisions, with decreases particularly concentrated among low‐income young adults. Untangling spatial dynamics reveals both important geographic commonalities and clear divergences. Declining young homeownership was found across 87% of municipalities, suggesting a common experience. Analyses also reveal stark spatial disparities, with the strongest decreases in the most urbanised regions, particularly large cities. Spatially divergent patterns of housing market exclusion are furthermore highly classed: widespread among young adults with a low income, while more spatially concentrated among those with a high income. Finally, using advanced spatial regression modelling, we explore municipal characteristics, revealing how local housing and population factors may explain pronounced differences in changing young adult homeownership entry. The analyses particularly emphasise links between housing unaffordability and competition as determinants of worsening homeownership opportunities for young adults.
Mapping Clinical Barriers and Evidence‐Based Implementation Strategies in Low‐to‐Middle Income Countries (LMICs)
Authors: Whitehorn Ashley , Fu Liang , Porritt Kylie , Lizarondo Lucylynn , Stephenson Matthew , Marin Tania , Aye Gyi Aye , Dell Kim , Mignone Alex , Lockwood Craig 
Published: May 2021

AbstractBackgroundLow‐to‐middle income countries (LMICs) experience a high burden of disease from both non‐communicable and communicable diseases. Addressing these public health concerns requires effective implementation strategies and localization of translation of knowledge into practice.AimTo identify and categorize barriers and strategies to evidence implementation in LMICs from published evidence implementation studies.MethodsA descriptive analysis of key characteristics of evidence implementation projects completed as part of a 6‐month, multi‐phase, intensive evidence‐based clinical fellowship program, conducted in LMICs and published in the JBI Database of Systematic Reviews and Implementation Reports was undertaken. Barriers were identified and categorized to the Donabedian dimensions of care (structure, process, and outcome), and strategies were mapped to the Cochrane effective practice and organization of care taxonomy.ResultsA total of 60 implementation projects reporting 58 evidence‐based clinical audit topics from LMICs were published between 2010 and 2018. The projects included diverse populations and were predominantly conducted in tertiary care settings. A total of 279 barriers to implementation were identified. The most frequently identified groupings of barriers were process‐related and associated predominantly with staff knowledge. A total of 565 strategies were used across all projects, with every project incorporating more than one strategy to address barriers to implementation of evidence‐based practice; most strategies were categorized as educational meetings for healthcare workers.Linking Evidence to ActionContext‐specific strategies are required for successful evidence implementation in LMICs, and a number of common barriers can be addressed using locally available, low‐cost resources. Education for healthcare workers in LMICs is an effective awareness‐raising, workplace culture, and practice‐transforming strategy for evidence implementation.
Rabies‐infected dogs at slaughterhouses: A potential risk of rabies transmission via dog trading and butchering activities in Vietnam
Authors: Vu Anh Hoang , Nguyen Thu Tuyet , Nguyen Dong Vinh , Ngo Giang Chau , Pham Thai Quang , Inoue Satoshi , Nishizono Akira , Nguyen Trong Duc , Nguyen Anh Kieu Thi 
Published: May 2021

AbstractThe study aimed to determine the status of neutralizing antibodies and rabies virus infection in dogs at slaughterhouses in Hanoi city from 2015 to 2017. A total of 2,376 pairs of blood and brain samples were collected from dogs at 92 slaughterhouses (8 large slaughterhouses and 84 small slaughterhouses) in 6 districts in Hanoi. Of the 1,500 dog samples from the large slaughterhouses where imported dogs were slaughtered, no dog brain samples were infected with rabies virus and no blood samples were positive for rabies neutralizing antibodies. Meanwhile, 7/876 (0.8%) of dog brain samples from small slaughterhouses in which slaughtered local dogs were positive for rabies virus and 26.4% had neutralizing antibodies against rabies virus. Analysis of the genetic characteristics of these rabies viruses showed that they all had a common origin with the domestic strains circulating in Northern Vietnam. Research results suggested that there was a potential risk of rabies transmission to humans through dog slaughtering activities in Vietnam. Vaccination for dogs should be strengthened, strictly implemented and frequently monitored to reach the rate of herd vaccination coverage as WHO’s recommendation. Dog trading and slaughtering activities should be strictly monitored to minimize the risk of rabies transmission to humans.
New Improved Method for Heat Transfer Calculation Inside Rough Pipes
Authors: Camaraza-Medina Yanan , Hernandez-Guerrero Abel , Luviano-Ortiz J. Luis 
Published: May 2021

AbstractAn improved method for heat transfer calculations inside rough tubes is provided. The model has been obtained from a second assessment developed early by the authors on fluid flow in single-phase inside rough tubes. The proposed correlation has been verified by comparison with a total of 1666 experimental available data of 34 different Newtonian fluids, including air, gases, water and organic liquids. The proposed model covers a validity range for Prandtl number ranging from 0.65 to 4.52×104, values of Reynolds number from 2.4×103 to 8.32×106, a range of relative roughness ranging from 5×10−2 to 2×10−6 , and viscosity ratio from 0.0048 to 181.5. The proposed model provides a good correlation for 2.4×103≤Re&lt;104 and  104≤Re≤8.32×106, with an average error of 18.3% for 70.4% of the data and 16.6% for 74.8% of the data, respectively. The method presents a satisfactory agreement with the experimental data in each interval evaluated; therefore, the model can be considered accurate enough for practical applications. At the present time, a method with similar characteristics is unknown in the available technical literature.
Experimental Investigation on Adiabatic Two-Phase Frictional Pressure Drop of R1234ze(E) and R134a in a Horizontal Minichannel
Authors: Xu Yu , Yan Zihao , Li Ling 
Published: May 2021

Abstract
R1234ze(E) has been created as an environmentally friendly substitution of R134a. An experimental investigation on adiabatic two-phase frictional pressure drop inside a 1.88 mm horizontal minichannel with R1234ze(E) and R134a was conducted. The saturation pressure and mass flux ranged within 0.6–0.9 MPa and 450–900 kg/(m2 s). Based on the 299 measured data points, the influences of various factors, including saturation pressure, mass flux, and the like, are examined detailedly. Larger mass flux brings about larger frictional pressure drop, and vapor quality performs the same function, but saturation pressure works inversely. The frictional pressure drops between R1234ze(E) and R134a present no significant differences. The experimental data are compared against 23 predictive models, among which the one from Xu and Fang (2013, “A New Correlation of Two-Phase Frictional Pressure Drop for Condensing Flow in Pipes,” Nucl. Eng. Des., 263, pp. 87–96) gives the best accuracy. In addition, a database including 3150 experimental adiabatic two-phase frictional pressure drop data points is derived from the current study and 29 published literature. Based on the database, the 23 models are assessed, and the most effective one is from Müller-Steinhagen and Heck (1986, “A Simple Friction Pressure Drop Correlation for Two-Phase Flow in Pipes,” Chem. Eng. Process., 20(6), pp. 297–308). This work can provide guidance for choosing appropriate two-phase frictional pressure drop models under adiabatic conditions.
Interactive Computational Medical Device Deployments within Virtual Reality
Authors: Deakyne Alex J. , Iaizzo Paul A. 
Published: May 2021

Abstract
3D modeling of anatomical features and medical devices are being used at increasing rates within the medical field. These 3D models allow for a wide variety of uses, such as educational or for medical device optimization. Next steps in such utilization are to perform computational and simulated device deployments within unique human anatomies. Such computational deployments have and will yield new perspectives and understandings relative to how given devices fit within the varied anatomies of varied patient populations. While these simulated device deployments offer many benefits, they are often time consuming to both develop and perform. Here, we present new functionalities to perform computational cardiac device deployments within virtual reality (VR). This functionality offers increased control of where the device is to be deployed, reducing the times required to perform computational device deployments in unique anatomical models.
Viscosity Matching Positively Affects the Correlation of Pressure-Volume Loops Between In-Vivo and Ex-Vivo Models
Authors: Schinstock Emma , Kudlik D’Anne , Eggen Michael , Iaizzo Paul A 
Published: May 2021

Abstract
Using Visible Heart® methodologies, swine hearts were reanimated and implanted with a left ventricular assist device (LVAD). System components were modified to better understand the relationships between the data collected and system conditions. The viscosity of the blood-replacement buffer was increased to a more physiologic value (~3 cP). Pressure-Volume (PV) loop recordings from each heart on the ex-vivo perfusion system more closely mimic loops recorded from the heart in-vivo. This indicates that any system aiming to characterize or examine LVAD performance must include a buffer which mimics blood viscosity or data must be adjusted for the relative viscosity of the buffer.
Design and Fabrication of a Focused Ultrasound Device for Minimallyinvasive Neurosurgery: Reporting a Second, Miniaturized and Mrcompatible Prototype with Steering Capabilities
Authors: Acord Molly , Kaovasia Tarana Parvez , Gamo Nao J. , Xiong Tim , Curry Eli , Aghabaglou Fariba , Morrison Kyle , Tyler Betty , Luciano Mark , Manbachi Amir 
Published: May 2021

Abstract
Many surgeons are faced with inoperable or only partially operable brain lesions, such as tumors. Even when surgery is feasible, patient outcomes are greatly affected by blood loss or infection. This has led many physicians toward non- or minimally-invasive surgery, which demands specialized toolkits. Focused ultrasound has great potential for assisting in such procedures due to its ability to focus a few centimeters away from the surface of the transducer. In a prior study, we developed a focused ultrasound prototype that could fit within a BrainPath trocar, specifically made for minimally invasive brain surgery. Here, we present the design and fabrication of a second prototype that reduces size, is MR-compatible, and has electronic steering capabilities.