bluuebunny/crossref_metadata_2025_split huggingface dataset:
UJI AKTIVITAS ANTIBAKTERI DAN ANTI-UV Phyllidiella nigra DAN BAKTERI SIMBIOTIKNYA DARI PERAIRAN TANJUNG MANDOLANG
Authors: Dajoh Tessalonica , Bara Robert A , Angkouw Esther , Ompi Medy , Lintang Rosita A , Lumenta Cykca 
Published: July 2021

Phyllidiella nigra is an organism that is suspected to have secondary metabolites because their ability to develop its self defense system by camouflage and using chemical compounds derived from their nature diet as deterrent against their predators. The purpose of this study was to isolate symbiotic bacterial derived from P. nigra, extracted and followed by, the antibacterial assays against Escherichia coli and Bacillus megaterium as well as the anti-UV assay. The results showed that the five isolates tested had an antibacterial activity with the highest average inhibition zone against E. coli DSM 498 bacteria, isolate 1 (14.67 mm), isolate 5 (14 mm), and against B. Megaterium DSM 32T bacteria, isolate 3 (13.33 mm). The three isolates which had the highest inhibition zone and P. nigra extract were tested for anti-UV assay using a UV-Vis Spectrophotometer. The results obtained isolate 3 has absorption of UV-A with the UV absorbtion maximum at λ 340 nm and P. nigra extract has absorption on UV-B radiation with UV absorption maximum at λ 290 nm. Key words: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV Phyllidiella nigra merupakan organisme yang diduga memiliki metabolit sekunder karena mampu mengembangkan sistem pertahanan dirinya dengan cara kamuflase dan menggunakan senyawa kimia sebagai racun yang didapat dari makanannya. Tujuan dari penelitian ini yaitu mendapatkan isolat bakteri yang bersimbiosis dengan P. nigra, mendapatkan ekstrak dari baktri simbion, dan menguji antibakteri dan anti-UV ekstrak etil aseta bakteri simbion dengan metode difusi agar terhadap bakteri Escherichia coli dan Bacillus megaterium. Hasil penelitian didapatkan kelima isolat yang diuji memiliki aktivitas antibakteri dengan rerata zona hambat tertinggi terhadap bakteri E. coli DSM 498 yaitu isolat 1 (14,67 mm), isolat 5 (14 mm), dan terhadap baktri B. megaterium DSM 32T yaitu isolat 3 (13,33 mm). Ketiga isolat yang memiliki zona hambat tertinggi dan ekstrak P. nigra diujikan anti-UV menggunakan alat UV-Vis Spektrofotometer. Hasil yang didapat isolat 3 memiliki serapan terhadap radiasi sinar UV-A dengan puncak tertinggi pada λ 340 nm dan ekstrak P. nigra memiliki serapan terhadap radiasi sinar UV-B dengan puncak tertinggi berada pada λ 290 nm. Kata kunci: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV
FILOGENI MOLEKULER ISOLAT BAKTERI F0-0-3-1 DARI MEDIA PEMELIHARAAN ROTIFER
Authors: Dalenoh Oktavianus , Wullur Stenly , Ginting Elvy L , Warouw Veibe , Rumampuk Detty N , Pangkey Henneke 
Published: July 2021

The aim of this study was to construct molecular phylogeny of bacteria suspected to involve in decomposing the fishery waste as diet for rotifer culture. The bacteria were isolated from culture of rotifer and propagated for molecular analysis. Genomic DNA of the bacteria was extracted using DNeasy Blood and Tissue Kit (Qiagen). The 16S rRNA gene was amplified using primer pairs i.e. 8F (AGAGTTTGATCCTGGCTCAG) and 1492R (GGTTACCCT GTTACGACTT) and sequenced. The sequences were analyzed using Sequence Scanner and MEGA 7, and BLASTed on the NCBI website (www.ncbi.nml.nih.gov). Molecular phylogeny of the isolate was constructed using Neighbor Joining Tree method. Isolate bacteria F0-0-3-1 from culture of rotifer fed with fish waste diet was successfully propagated for molecular analysis. 16S rRNA gene of the isolate bacteria was successfully amplified and showed a DNA band at 1400 bp. Nucleotides sequence quality of the 16S rRNA gene i.,e QV+20 and CRL were 995 and 941 nucletides. BLAST result of the 16S rRNA gene showed 98.87% percent identity of the isolate bacteria F0- 0-3-1 with bacterial species in the genus Bacillus i.e. Bacillus weidmanni, Bacillus cereus dan Bacillus proteolyticus. Molecular phylogeny analysis showed that the three species was in the same clade.Keywords: Phylogeny, molecular, bacteria, rotifer, 16S rRNA gene Penelitian ini bertujuan untuk mengkonstruksi filogeni molekuler bakteri yang diduga terlibat dalam proses penguraian limbah perikanan sebagai pakan untuk kultur rotifer. Isolat bakteri yang diperoleh dari kultur rotifer tersebut, dibiakkan dan DNA genomnya diekstrak menggunakan DNeasy Blood and Tissue Kit (Qiagen). Gen 16S rRNA isolat bakteri tersebut, diamplifikasi menggunakan primer 8F (AGAGTTTGATCCTGGCTCAG) dan 1492R (GGTTACCCT GTTACGACTT) selanjutnya, disekuens dan urutan nukleotida hasil sekuens dianalisis menggunakan program Sequence Scanner dan MEGA 7. Analisis homologi sekuens dilakukan dengan program BLAST nucleotide blast, pada situs NCBI (www.ncbi.nml.nih.gov) dan dilanjutkan dengan konstruksi filogeni molekuler menggunakan metode Neigbor Joining Tree. Isolat bakteri F0-0-3-1 berhasil disolasi dari kultur rotifer yang diberi pakan limbah ikan. Hasil amplifikasi Gen 16S rRNA isolat bakteri F0-0-3-1 terdeteksi dalam bentuk pita DNA pada posisi sekitar 1400 bp. Kualitas nukleotida gen 16S rRNA hasil sekuens menunjukan nilai QV 995 dan CRL 941. Hasil BLAST sekuens gen 16S rRNA isolat bakteri F0-0-3-1 pada database menunjukkan kemiripan 98% dengan spesies Bacillus wiedmanni. Hasil kontruksi filogeni menggunakan metode Neighbor Joining Tree menunjukan posisi isolat bakteri F0-0-3-1 berada pada clade yang sama dengan Bacillus weidmanni, Bacillus cereus dan Bacillus proteolyticus. Kata kunci: Filogeni, molekuler, bakteri, rotifer, Gen 16S rRNA
ISOLASI JAMUR SIMBION ASCIDIA Schizophyllum commune YANG MEMILIKI AKTIVITAS ANTIBAKTERI
Authors: Montolalu Gianfranco , Sumilat Deiske A , Rumampuk Natalie D.C. , Rumengan Inneke FM , Lintang Rosita AJ , Kreckhoff Renie Lucia 
Published: July 2021

Ascidian is a benthic invertebrate that produces secondary metabolites. The substances were produced by the ascidian as self-defense from many environmental factors. Several studies report the similar structure between the substances extracted from ascidian and symbiotic fungi with biological activities. This study aims to isolate fungi associated with ascidian Eudistoma sp., then observe its potency on inhibiting bacteria S. aureus dan A. hydrophila. PDA was used to isolate and culture fungal isolate, then extraction using solvent ethyl acetate. Antibacterial activity of this symbiotic fungi isolates extract was performed using the Kirby-Bauer disc diffusion method. Molecular identification of fungi results that the symbiotic fungi were Schizophyllum commune. The observing result showed that fungal extract has the ability on inhibiting the growth of S. aureus and with the inhibition zone 8 mm and A. hydrophila 8,5 mm.Keywords: Ascidian, Eudistoma sp., Schizophyllum commune, Symbiotic, Antibacterial
FILOGENI MOLEKULER BAKTERI DARI MEDIA PEMELIHARAAN ROTIFER YANG DIBERI OLAHAN LIMBAH IKAN SEBAGAI SUMBER NUTRISI
Authors: Hubu Herlin S , Wullur Stenly , Warouw Veibe , Ginting Elvy L , Bara Robert A , Wantasen Adnan S 
Published: July 2021

This study aims to identify and construct molecular phylogeny of an isolate bacteria from culture media of rotifer Brachionus rotudiforis supplied with processed fishery waste feed as nutritional source. The use of fish waste-based food for rotifer showed positive effects on growth and nutrient content of the rotifers. Genomic DNA of the isolate bacteria BRLI- 01 was extracted and the 16S rRNA gene was amplified using primers (8F and 1492F) and further sequenced using Sanger sequence technique. The 16S rRNA gene was analysed using SeqScanner® and MEGA® followed with BLAST (Basic Local Alignment Search Tool) analyses in the NCBI (National Centre for Biotechnology Information). Amplification result of 16S rRNA gene bacteria s NCBI site as a reference for identification and phylogeny of bacterial species. BRLI-01 was successfully cultured on rotifer rearing media. The results of the 16S rRNA gene amplification of the isolate bacteria showed a DNA band with a length of 1400 bp. The BLAST result on the NCBI showed that the isolate bacteria BRLI-01 had a percent identity (98.46%) and is in the same phylogony branching position with Vibrio rotiferianus Keywords: Rotifers, Bacteria, Fish waste, 16S rRNA Genes, Phylogeny identification
BIOINDEKS ZOOPLANKTON DI PERAIRAN PULAU BUNAKEN
Authors: Tampi Kezia Keren Erika , Rimper Joice R.T.S.L , Warouw Veibe 
Published: July 2021

The city of Manado has several islands which are strategic places with the development of marine potential. One that is so potential is the waters of Bunaken Island and the forms of activity in Bunaken Island waters vary widely. The purpose of this study is to determine the abundance value and biological index and to analyze the condition of the waters in the waters and to see the comparison at each research station. The results of zooplankton identification consisted of 7 genera including all research stations. The results of the analysis of the highest abundance data were at the third station (8.4822 ind / l) and the lowest abundance value was at the first station (7.4220 ind / l). The results of the biological index analysis obtained are as follows: the average diversity value at three stations is 1.08, the uniformity is 0.98 and the dominance is 0.20. The results of the Morisita index for each station are: stations. Which means the value of the distribution pattern is three groups, and the others are uniform in each research genus, the overall distribution is uniform.Keywords: Zooplankton, Bunaken Island waters, Abundance, Bioindex
STRUKTUR KOMUNITAS DAN ZONASI VEGETASI MANGROVE DESA DARUNU KECAMATAN WORI KABUPATEN MINAHASA UTARA
Authors: Upara Udin , Kusen Janny Dirk , Sondak Calvyn F.A. , Schaduw Joshian N.W , Tilaar Sandra Olivia , Lasabuda Ridwan 
Published: July 2021

Mangrove ecosystem is a collection of distinctive plant types that contained in tropical and subtropical coastal region. The purpose of research was to find out the community structure and mangrove zonation at Darunu Village, Wori District, North Minahasa Regency. The research was conducted by the quadrant transect line method. It was done by pulling a straight line, perpendicular from the outermost mangrove area to the land direction, along 100 m by determining three data retrieval Station. To determine the condition of mangrove then density, species frequency, species coverage, important value index, diversity index and equality types were analyzed. The result found 5 mangroves species, namely: Rhizophora apiculata, R. mucronata, Sonneratia alba, Bruguiera gymnorrhiza and Avicennia officinalis. The highest density value was R.apiculata, as well as species frequency, while the species coverage value found in S. alba. The diversity value found at Station 2. The zonation of mangrove vegetation in Darunu village respectively, at Station 1 and 2 in the front was overgrown by R.apiculata species with sandy mud substrate. The Station 3 was overgrown by S.alba species with sandy mud. Generalyl, this location substrate contains of sandy mud and mud.Keywords : Mangrove, community structure, zonation.
KARAKTERISTIK GELOMBANG DI BEBERAPA KAWASAN PERAIRAN SEKITAR SEMENANJUNG MINAHASA
Authors: Ginting Intanta Br , Angmalisang Ping Astony , Rampengan Royke M , Djamaluddin Rignolda , Manengkey Hermanto WK , Wantasen Adnan S 
Published: July 2021

The waters surrounding the Minahasa Peninsula are important areas of various activities by use of coastal and marine space. This research aims, firstly to describe direction, period and height of waves at several water areas around the Minahasa Peninsula in various season. Secondly to analyze the characteristic of daily and seasonal waves. The wave parameter, which reanalysis by ECMWF for 5 years (September 2014 to august 2019) were used in this research. By the analysis spasial-temporal, the results are presented by the rose grapich waves direction and stock chart. The wave propagation in the north season and transition season I, is dominated by the northeasterly wave. While the wave propagation in the southern season and the transitional season II, is more diverse directions. The characteristics of the waves formed in the southeast Minahasa waters in the north season and transitional season I, show that the wave period is greater than in the waters of Manado Bay and Bitung waters. Furthermore, the wave characteristics formed in the waters of the Minahasa Peninsula show a significant wave height in Bitung waters which is higher than the waters of Manado Bay and Southeast Minahasa waters.Keywords: Waves, ECMWF, Minahasa Peninsula Waters Perairan sekitar Semenanjung Minahasa merupakan kawasan penting dalam berbagai aktivitas pemanfaatan ruang pantai dan laut. Penelitian ini bertujuan, pertama untuk mendeskripsikan arah, periode, dan tinggi gelombang di beberapa kawasan perairan sekitar Semenanjung Minahasa dalam berbagai musim yang berlangsung. Kedua adalah menganalisis karakteristik gelombang harian dan musiman pada perairan sekitar Semenanjung Minahasa. Data yang digunakan dalam penelitian adalah data reanalysis ECMWF (European Centre for Medium-Range Weather Forecasts) parameter gelombang selama 5 tahun mulai bulan September 2014 sampai Agustus 2019. Dengan metode analisis spasial-temporal hasilnya disajikan dalam bentuk grafik mawar arah datang gelombang dan grafik kotak (stock chart). Rambatan gelombang pada musim Utara dan musim Peralihan I, arah datangnya didominasi dari arah Timur Laut. Sedangkan rambatan gelombang pada musim Selatan dan musim Peralihan II, arah datangnya lebih beragam. Karakteristik gelombang yang terbentuk di perairan Minahasa Tenggara pada musim Utara dan musim Peralihan I memperlihatkan periode gelombangnya lebih besar dari pada perairan Teluk Manado dan Perairan Bitung. Selanjutnya karakteristik gelombang yang terbentuk di perairan Semenanjung Minahasa memperlihatkan tinggi gelombang signifikan di perairan Bitung lebih tinggi dari pada perairan Teluk Manado dan perairan Minahasa Tenggara.Kata kunci: Gelombang, ECMWF, Perairan Semenanjung Minahasa
KAJIAN KOMUNITAS EKOSISTEM LAMUN DI SEMENANJUNG TARABITAN KECAMATAN LIKUPANG BARAT KABUPATEN MINAHASA UTARA
Authors: Sitaba Risandi D , Paruntu Carolus P , Wagey Billy Theodorus 
Published: July 2021

This research was conducted in the waters of Tarabitan Peninsula, West Likupang North Minahasa using quadants transect method. The purpose of this study was to determine the community structure of seagrass found in that waters as initial information for sustainable management seagrass ecosystem . Field observation was conducted to identify the seagrass species, number of individuals/shoots, percent cover for each type of seagrass in those plotting quadrants. The result of this study documented 6 types of seagrass namely, Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halophila ovalis and Halodule uninervis. The species composition and distribution of seagrass were varied and was dominated by Thalassia hemprichii was the most dominant seagrass species with a relative density of 55.55%, a relative frequency of 33.67%, 39.92% relative cover, an important value index of 129.03%, a diversity index of 1.30 belonging to this condition, moderate, the uniformity index of 0.72 is classified as high and the dominance index of 0.2 is classified as low. Based on Minister of Environment Decree Republic Indonesia No. 200 of 2004 concerning the status of seagrass beds, the condition of the seagrass beds in the waters of Tarabitan Village is classified as rich / healthy with a cover value of ≥ 60. Keywords : Seagrass Community, Species Composition, distribution, Tarabitan Peninsula Penelitian ini dilakukan di perairan Semenanjung Tarabitan Likupang Barat Minahasa Utara dengan menggunakan metode transek kuadran. Tujuan penelitian ini adalah untuk mengetahui struktur komunitas lamun yang terdapat di perairan tersebut sebagai informasi awal untuk pengelolaan lamun secara berkelanjutan. Pengamatan lapangan dilakukan untuk mengidentifikasi jenis lamun, jumlah individu/tegakan, persentase tutupan tiap jenis lamun pada tiap kuadran. Hasil penelitian ini mendokumentasikan 6 jenis lamun yaitu Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halophila ovalis dan Halodule uninervis. Komposisi jenis dan sebaran lamun bervariasi dan didominasi oleh jenis lamun Thalassia hemprichii merupakan jenis lamun yang paling dominan dengan kerapatan relatif 55,55%, frekuensi relatif 33,67%, tutupan relatif 39,92%, indeks nilai penting 129,03%, indeks keanekaragaman 1,30 tergolong dalam kondisi sedang, indeks keseragaman 0,72 tergolong tinggi dan indeks dominansi 0,2 tergolong rendah. Berdasarkan Keputusan Menteri Lingkungan Hidup Nomor 200 Tahun 2004, kondisi padang lamun di perairan Desa Tarabitan tergolong kaya / sehat dengan nilai tutupan ≥ 60.Kata Kunci: Komunitas Lamun, Komposisi Jenis, Distribusi, Semenanjung Tarabitan
PENAMBATAN MOLEKUL GLUTATION FAUNA LAUT TERHADAP RESEPTOR DARI BEBERAPA PENYAKIT VIRUS
Authors: Kai Q Xaxa Alvin , Rumengan Inneke FM , Lintang Rosita AJ , Wullur Stenly , Sumilat Deiske A , Pangkey Henneke , Luntungan Hein A 
Published: July 2021

Glutathion is a tripeptides produced by almost all living creatures, including marine fauna such as rotifers and shrimps. Judging from the important molecular role of glutathion in living cell, the ability of glutathion as antiviral compound, has been assed by application of the AutodockTools to predict the ability of glutathion as ligand to bind receptors of several viral diseases such as covid-19, malaria, and hepatitis B. Docking results of glutathion toward target receptor were shown by the parameter, Gibbs energy value, and supported by other data such as the amount of hidrogen bond, amino acid residues, and bond distance. Evidently, the glutathion could bind to each receptor of covid-19, malaria and hepatitis B, with values of Gibbs energy (∆G), -7,9, 7,1 and -7,1 kcal/mol, respectively. This proves that glutathion has ability to bind covid-19 receptor, even stronger than its ability to bind receptors of other viral diseases, and to the remdesevir. Between glutathion and covid-19 receptor, there were 11 hidrogen bonds, with distance ranged from 2,76 to 3,25 Å. These were stronger than those between glutathion and receptors of malaria and hepatitis B, only 8 and 4 Å hidrogen bonds, respectively. To develop glutathion as antiviral drug, it is necessary to carry out in vitro and in vivo analyses, prior to clinical test. Therefore, it requires to explore the marine fauna species that produce glutathion and their cultivation.Keywords: molecular docking, glutathion, marine fauna, autodock vina, viral diseases, binding affinity
KELIMPAHAN DAN KEANEKARAGAMAN JENIS-JENIS KEPITING DI SUBSTRAT MANGROVE PERAIRAN MERAS DAN MOLAS KECAMATAN BUNAKEN KOTA MANADO SULAWESI UTARA
Authors: Wuisan Rivaldo A , Kaligis Erly 
Published: July 2021

Crab of the genus Uca is one type of crustacean that makes mangroves a habitat to carry out their life cycle in order to remain sustainable. The purpose of this research is to identify the first, the second is to calculate the abundance, species diversity, dominance, and the third is to know the state of the aquatic environment around pH, temperature, salinity and substrate. The method used is the cruising method and the quadratic line transect method. The method of roaming as far as 300 m. While the line transect method is carried out by drawing three transect lines along 28 m. The identification results in Meras waters found 6 species. Based on data analysis using the cruising method, the number of individuals in Meras waters is 383 individuals, while in Molas waters there are 210 individuals. Based on data analysis using the quadratic line transect method, the species abundance index in Meras waters is 6,767 ind/m2, while in Molas waters it is 4,533 ind/m2. The value of H' at two locations is moderate. The value of C in two locations is low. Keywords: Meras, Molas, Crabs, abundance, species diversity, dominance.
Immunotherapy in Special and Rare Situations: A Brief Review
Authors: Srinivas Sujay , Bajpai Jyoti 
Published: July 2021

ABSTRACT
Immunotherapy has established itself as an important component of the treatment armamentarium against various solid as well as hematologic cancers. Immune checkpoint inhibitors (ICI) provide for a very well-tolerated and efficacious treatment option that has improved survival in several cancers. The approved ICIs mainly consist of antibodies targeting cytotoxic T lymphocyte–associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1) or its ligand, programmed cell death ligand 1 (PD-L1). However, most clinical trials of ICI have excluded patients from high-risk populations, such as those with autoimmune diseases, patients on chronic steroid intake for various reasons or preexisting HIV infections. The older adults are also an underrepresented section of the population enrolled into such trials, most probably due to the higher prevalence of comorbidities and frailty affecting their Eastern Co-Operative Oncology Group performance status, and thus the eligibility for clinical trial enrollment. This paper aimed to briefly review the available evidence and thus guide the decision-making process for use of ICI in such rare and special situations.
Inovasi Produk Olahan Kedele pada UMKM Kedele Cryspy Erte
Authors: Kadafi Asroful , Suharni Suharni , Christiana Ratih 
Published: July 2021

Usaha Mikro Kecil dan Menengah (UMKM) pada masa pandemic Covid-19 adalah salah satu sektor ekonomi yang terdampak. Masalah ini juga dialamai oleh Mitra Program Pengabdian Masyarakat kami. UMKM mitra kami yang memproduksi olahan kedelai, memiliki beberapa permasalahanj diantaranya: pertama: produksi dilakukan masih dengan cara tradisional, kedua: belum memiliki ijin usaha, dan ketiga: belum memiliki ijin PIRT. Untuk menyikapi masalah ini, perlu adala solusi membantu permasalahan Mitra. Dari permasalahan yang ada Tim Pengabdian Masyarakat (Abdimas) merencana metode pelaksanaan diantaranya: melaksanakan pelatihan dan pendampingan kepada mitra (pengolahan, pengemasan dan pemasaran), dan melakukan pendampingan pengurusan ijin administrasi mitra. Hasil yang dicapai dari program ini diantaranya: Usaha Mitra mengalami perubahan yang cukup siginifikan, ditandai dari olahan produk kedele yang dulunya hanya kedele goreng dikemas dalam kemasan sederhana sekarang menjadi olahan kedele krispi yang jauh memiliki nilai ekonomi tinggi, diperolehnya Ijin PIRT dan Ijin Usaha.

Abstract
UMKM during the Covid-19 pandemic was one of the economic sectors affected. This problem is also experienced by our Community Service Program Partners. Our partner MSMEs that produce processed soybeans, have several problems including: first: production is still carried out traditionally, second: does not have a business permit, and third: does not have a PIRT permit. To address this problem, a solution is needed to help Partner's problems. From the existing problems, the Community Service Team (Abdimas) plans implementation methods including conducting training and mentoring to partners (processing, packaging, and marketing) and providing assistance in the management of partner administrative permits. The results achieved from this program include: Partners' businesses have undergone significant changes, marked from processed soy products which were previously only fried soybeans packaged in simple packages, now processed crispy soybeans which have far high economic value, obtaining PIRT permits and business permits