bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening the Hub Genes and Analyzing the Mechanisms in Discharged COVID-19 Patients Retesting Positive through Bioinformatics Analysis
Authors: Fang Ke-Ying , Liang Gui-Ning , Zhuang Zhuo-Qing , Fang Yong-Xin , Dong Yu-Qian , Liang Chuang-Jia , Chen Xin-Yan , Guo Xuguang 
Published: November 2021

Abstract
Background: With the worldwide spread of COVID-19, people’s health and social order have been exposed to enormous risks. After encountering patients who test positive again after discharge, our study analyzed the pathogenesis to further assess the risk and possibility of virus reactivation.Methods: A separate microarray was acquired from the Integrated Gene Expression System (GEO), and its samples were divided into two groups: a “convalescent-RTP” group consisting of recovery and “retesting-positive” (RTP) patients (group CR) and a “health-RTP” group consisting of healthy control and RTP patients (group HR). The enrichment analysis was performed with R software, obtaining the gene ontology (GO) and Kyoto pluripotent stem cells (KEGG) of the genes and genomes. Subsequently, the protein–protein interaction (PPI) networks of each group were established and the hub genes were discovered using the cytoHubba plug-in.Results: In this study, 20 differentially expressed genes were identified, and 6622 genes were identified in the group CR, consisting of 5003 up-regulated and 1619 down-regulated genes. Meanwhile, 7335 genes were screened in the group HR, including 4323 up-regulated and 3012 down-regulated ones. The GO and KEGG analysis of the two groups revealed significant enrichment of these differentially expressed genes in pathways associated with immune response and apoptosis. In the PPI network constructed, 10 hub genes in group CR were identified, including TP53BP1, SNRPD1, SNRPD2, SF3B1, SNRNP200, MRPS16, MRPS9, CALM1, PPP2R1A, YWHAZ. Similarly, TP53BP1, RPS15, EFTUD2, MRPL16, MRPL17, MRPS14, RPL35A, MRPL32, MRPS6, POLR2G were selected as hub genes.Conclusions: Using the messenger ribonucleic acid (mRNA) expression data from GSE166253, we explore the pathogenesis of retesting positive in COVID-19 from the immune mechanism and molecular level. We found TP53BP1, SNRPD1 and SNRPD2 as hub genes in RTP patients. Hence, their regulatory pathway is vital to the management and prognostic prediction of RTP patients, rendering the further study of these hub genes necessary.
A Closed-loop Control for Swing System and Energy Regeneration Analyses of the Multi-system of Grasp Steel Machine
Authors: Yan Yunqi , Wang Puquan , Xu Yun , Shi Yan , Liao Yinghua 
Published: November 2021

Abstract
Facing the environment problems, the improvement on the efﬁciency of the construction machinery is highly demanded, meanwhile, large energy wasted in hydraulic slewing systems, reaching the desired speed slowly and vibration in braking are widespread. In this paper, a closed-loop control swing system (CCSS) is proposed. In this system, bidirectional variable pump controls the change of oil flow by programmed software algorithm, instead of pilot actuated reversing valve, which signiﬁcantly improves the efficiency of hydraulic circuit. Based on the model structures, we develop a hydraulic swing prototype and verify the advantages of our design means of theoretical calculation and simulation. It verifies that CCSS and based on accumulator slewing system(BASS) can respectively save 875.5 KJ and 347.2 KJ more than OCSS with operational weight of 100 tons grasp steel machine, which can support theoretical basis for energy conservation and environmental protection to study the new type of engineering machinery.
Semen Parameters Are Seriously Affected in Acephalic Spermatozoa Syndrome
Authors: Ying Li-juan , Liu Qing-ting , Yu Lin , Yang Tingting , Wu Ying-bi , Xu Jing-yan , Jia Ye-lin , Zheng Yan , Deng Dong , Li Fu-ping 
Published: November 2021

Abstract
Objective: To evaluate the effect of acephalic spermatozoa syndrome on sperm quality in semen with different proportions of headless sperm.Design: Case control study.Setting: Andrology Laboratory.Patient(s): A total of 391 patients with headless sperm and 400 prenatal examination patients with no headless sperm who underwent semen analysis at the andrology laboratory.Intervention(s): None.Main Outcome Measure(s): The correlation of the proportion of headless sperm in semen with semen parameters.Result(s): All semen parameters except the semen volume were negatively (P<0.05) correlated with the proportion of headless sperm in the semen. The semen samples were divided into three groups based on the proportion of headless sperm (PHS) as follows: 0<PHS≤10% (n=249, group A), 10<PHS≤20% (n=71, group B) and PHS>20% (n=71, group C). Nearly all semen parameters were significantly lower in group B and group C than in the control group (P<0.05). However, in group A, only the vitality and motility parameters were lower than those of the control group.Conclusion(s): Semen samples containing headless sperm tend to have lower semen parameters than samples without headless sperm. Increases in the proportion of headless sperm in semen samples are associated with decreases in semen quality.
Recruitment of Regulatory T Cells with rCCL17 Promotes M2 Microglia/Macrophage Polarization Through TGFβ/TGFβR/Smad2/3 Pathway in a Mouse Model of Intracerebral Hemorrhage
Authors: Deng Shuixiang , Jin Peng , Liu Shengpeng , Huang Lei , Sherchan Prativa , Cui Yuhui , Zhang John H. , Gong Ye , Tang Jiping 
Published: November 2021

Abstract
Background
Intracerebral hemorrhage (ICH) is a devastating neurological disease with high mortality and morbidity. The microglia activation and peripheral inflammatory cells infiltration play an important role in the ICH prognosis. Previous studies have demonstrated that regulatory T cells (Tregs) ameliorated neuroinflammation following experimental ICH. However, the specific molecular mechanism underlying such effects of Tregs remains unclear. In the present study, our aims were to examine the effect of Tregs recruitment induced by recombinant CC chemokine ligand 17(rCCL17) in an intrastriatal autologous blood mouse model of ICH and to determine whether the TGF-β/TGF-βR/Smad2/3 pathway was involved in Tregs promoted M2 microglia/macrophage polarization.
Methods
A total of 404 adult CD1 mice (male, eight-week-old) were subject to sham surgery or autologous blood injection of ICH. A CD25-specific mouse antibody or isotype control mAb was injected intraperitoneally 48h prior to ICH induction to deplete Tregs. Recombinant CCL17 (rCCL17), a C-C chemokine receptor 4 (CCR4), was delivered intranasally at 1 h post-ICH. SB431542, a specific inhibitor of TGF-β was administered intraperitoneally 1 h before ICH induction. Post-ICH assessments included neuro-behavior evaluation, brain edema, hematoma volume, hemoglobin content, western blotting, double immunofluorescence staining and immunohistochemistry.
Results
Endogenous brain expressions of CCL17 and Tregs marker Foxp3 as well as the number of Tregs in the perihematomal region were increased following ICH. The Tregs deletion by a CD25 antibody aggravated short-term neurological deficits and brain edema, increased the level of inflammatory cytokines and peripheral inflammatory cells infiltration, exacerbated hematoma expansion and increased M1phenotypes of microglia/macrophage in ICH mice. The rCCL17 treatment increased the number of Tregs in the brain, reduced hematoma expansion and brain edema, promoted microglia/macrophage polarization toward M2 phenotypes. Moreover, the expressions of brain TGF-β/phosphorylated-Smad2/3 were increased. The neuroprotective effects of rCCL17 were abolished by co-administration of the selective TGF-β inhibitor SB431542.
Conclusions
Our study demonstrated rCCL17 recruited of Tregs to brain in the autologous blood injection model of ICH. Tregs promoted microglia/macrophages polarization toward M2 phenotype and alleviation early brain injury, at least in part, through the TGFβ/TGFβR/Smad2/3 signaling pathway in ICH mice. Thus, rCCL17-mediated Tregs recruitment may provide a promising therapeutic strategy to reduce early brain injury after ICH.
"Net cage" technique in the treatment of inferior pole patella fracture: a novel patella internal fixation technique
Authors: Li Ming , Qi Hongfei , Ma Teng , Li Zhong , Ren Cheng , Lu Yao , Xue Hanzhong , Yang Yanling , Zhang Kun 
Published: November 2021

Abstract
Objective

Fractures of the inferior pole of the patella can cause the knee joint extensor mechanism disorder. The fracture fragments are usually small and comminuted. Therefore, there are certain difficulties in fixation. The purpose of this study is to observe the effect of the "Net cage" technique in the treatment of the inferior pole of the patella.
Methods

This is a retrospective study that included 16 cases of inferior patella fractures (AO/OTA 34-A1) who underwent the "Net cage" technique from March 2017 to June 2020. Collecting their medical records and follow-up results, measuring indicators include the surgical complications of the fixation method, knee joint function, the number of fluoroscopies, fracture healing, and the incidence of soft tissue stimulation.
Results

The fractures of all patients healed smoothly, and there were no complications such as failure of internal fixation and fracture of implants. The average number of intraoperative fluoroscopy was 5.56 times (range: 4-10 times); the average fracture healing time was 10.5 weeks (range: 8-14 weeks) ). No patients reported internal fixation-related soft tissue irritation. At the last follow-up, the knee function showed that the average ROM was 133.75° (range: 120°~140°); The average Bostman score was 27.94 points (range: 24-30 points).
Conclusion

"Net cage" technology for the treatment of inferior pole fractures of the patella has the advantages of reduced fluoroscopy, fixation and stability, early functional exercise, and better knee joints after surgery.
The influence of risk perceptions on close contact frequency during the SARS-CoV-2 pandemic
Authors: Wambua James , Hermans Lisa , Coletti Pietro , Verelst Frederik , Willem Lander , Jarvis Christopher , Gimma Amy , Wong Kerry , Lajot Adrien , Demarest Stefaan , Edmunds W. John , Faes Christel , Beutels Philippe , Hens Niel 
Published: November 2021

Abstract
Human behaviour is known to be crucial in the propagation of infectious diseases through respiratory or close-contact routes like the current SARS-CoV-2 virus. Intervention measures implemented to curb the spread of the virus mainly aim at limiting the number of close contacts, until vaccine roll-out is complete. Our main objective was to assess the relationships between SARS-CoV-2 perceptions and social contact behaviour in Belgium. Understanding these relationships is crucial to maximize interventions' effectiveness, e.g. by tailoring public health communication campaigns. In this study, we surveyed a representative sample of adults in Belgium in two longitudinal surveys (8 waves of survey 1 in April 2020 to August 2020, and 11 waves of survey 2 in November 2020 to April 2021). Generalized linear mixed effects models were used to analyse the two surveys. Participants with low and neutral perceptions on perceived severity made a significantly higher number of social contacts as compared to participants with high levels of perceived severity after controlling for other variables. Furthermore, participants with higher levels of perceived effectiveness of measures and perceived adherence to measures
made fewer contacts. However, the differences were small. Our results highlight the key role of perceived severity on social contact behaviour during a pandemic. Nevertheless, additional
research is required to investigate the impact of public health communication on severity of COVID-19 in terms of changes in social contact behaviour.
Risk Prediction of Death in Inpatient Adults With COVID-19 from Mexico
Authors: Martínez-Vega Ruth Aralí , Jing Wang , Ortega-Villa Ana Maria , Delgado-Cuellar Oscar Manuel , Hernández-Hernández Victor Alonso , Jan-Gómez Julio Cesar , Rincón-León Héctor Armando , Constantino-Santiesteban Paul , García-Guerra Mariana Piedad , Cetina-Díaz José Hiram , Pérez-Tirado José Manuel , Gómez-Cruz Omar , Amaya-Larios Irma Yvonne , Ramos-Castañeda Jose , Jesús Sepúlveda-Delgado 
Published: November 2021

Abstract
Background

There is substantial variation in COVID-19 lethality across countries. In addition, in countries with populations with extreme economic inequalities, such as Mexico, there are regional and local differences in risk factors for COVID-19 death. The goal of this study was to test the hypothesis that the risk of death in Mexican COVID-19 patients was associated with the time between symptom onset and hospitalization and/or with the healthcare site. Also, death prognostic models were developed.
Methods

The study included two COVID-19 inpatient cohorts, one prospective and one retrospective from Chiapas, Mexico. Demographic, clinical and laboratory variables were collected, and the diagnosis of SARS-CoV-2 infection was performed using RT-qPCR in samples collected seven days since symptom onset. The 30-day mortality, since symptom onset, was the outcome, and clinical variables at the first 48 hours of hospitalization were independent factors. Multivariate logistic regression analyses were conducted.
Results

Of the 392 patients included, 233 died (59.4%). The time between symptom onset and hospitalization, the healthcare site and sex were not related to the 30-day mortality. Three death prognostic models were developed (AUC between 0.726 and 0.807). Age, LDH, AST, and lymphocyte count were included in all models, OSI-WHO Classification (Non-invasive ventilation or high-flow oxygen, and mechanical ventilation with or without organ support/ECMO) and leukocyte count in two models, and diabetes and diarrhea in one model.
Conclusion

The population evaluated had underlying deteriorated health before COVID-19 compared with regional and country population. The factors that determine the COVID-19 mortality risk in a relatively healthy population are sex, age and comorbidities. However, as this study shows, when populations have underlying poor health, some of these factors lose their associations with mortality risk, and others become more important.
Cellular Damage After Prolonged Low-Dose Exposure of Neonicotinoid in Rats
Authors: Naseer Rahat , Tariq Affan , Mirza Munazza Raza , Rashid Muhammad , Raza Syed Qasim , Rajput Sajid Ali 
Published: November 2021

Abstract
Background; Dinotefuran is a new class of neonicotinoids claimed to be harmless to mammals and humans. This claim was daunted by the documented effect of dinotefuran on honeybees and further studies were required. Aim: The study was designed to assess the capaciousness of damage caused by prolonged exposure of dinotefuran in mammals and probable strategy to neutralize its effect. Methodology: Ninety-day trial using Wistar rats (n=45) was conducted while dividing them into three groups: untreated control group, insecticide (dinotefuran) treated group, and dinotefuran treated and vitamin E supplemented group. Dinotefuran was administrated orally (LD25). Vitamin E (alpha-tocopherol) supplementation was given in water ad libitum. Blood sampling was done twice a month, and hematological and biochemical data were recorded. After expiry of trial period, the experimental rats were anesthetized and sacrificed. Organs (kidneys, liver, and heart) were isolated from each groups, weighed, and stored at approximately -20°C till further processing, analysis and histopathology were performed. Results: All the hematological parameters were affected significantly. Histopathology of tissues showed clear necrosis in all the tissues except kidneys. All the biomarkers of oxidative stress and comet assay demonstrated significant cell damage. All the parameters showed improvement after vitamin E supplementation but non-significantly. Significance: These findings were suggestive that even low dose persistent exposure can lead to mutagenicity and carcinogenicity in mammals and other non-target species hence revised policy guidelines and more intelligent use of these chemicals is required.
Evaluation of the inhibitory potential of essential oils and aromatic extracts on SARS-CoV-2 Mpro and PLpro
Authors: Strub Daniel , Talma Michał , Strub Maria , Rut Wioletta , Żmudziński Mikołaj , Brud Władysław , Zhang Linlin , Sun Xinyuanyuan , Lv Zongyang , Nayak Digant , Olsen Shaun , Hilgenfeld Rolf , Drag Marcin 
Published: November 2021

Abstract
Essential oils and aromatic extracts (oleoresins, absolutes, concretes, resinoids) are often used as food flavorings and constituents of fragrance compositions. The flavor and fragrance industry observes significant growth in sales of some natural materials during the COVID-19 outbreak. Some companies worldwide are making false claims regarding their essential oils or blends to be effective (or indirectly points towards this conclusion) against coronaviruses even though the available data on the activity of plant materials against highly pathogenic human coronaviruses is very scarce. Our exploratory study aimed to develop pioneering knowledge, and provide the first experimental results on the inhibitory properties of hundreds of flavor & fragrance materials against SARS-CoV-2 main- and papain-like proteases. As essential oils are volatile products, they could provide an interesting subsidiary inhalation therapeutic strategy in the long term.
Application of a herbal preparation in the therapy of acute tonzillopharyngitis in children
Authors: Borisova O. V. , Bochkareva N. M. , Polezhaeva N. S. , Frank A. A. , Yashkina O. N. 
Published: November 2021

Introduction. Tonsillopharyngitis often accompanies the course of acute respiratory infections in children (up to 12-15%). The use of systemic antibacterial drugs for viral etiology of the disease has not been shown, therefore, the interest of researchers in the use of herbal products is increasing. In numerous clinical trials conducted around the world, the liquid extract of the roots of Pelargonium sidoides EPs® 7630 has been shown to be effective and safe in the treatment of acute respiratory infections, acute tonsillopharyngitis, rhinosinusitis, acute bronchitis in adults and children.Objective of the study: assessment of the efficacy and safety of the drug EPs® 7630 (Umckalor) in acute tonsillopharyngitis of non-streptococcal etiology in children 2-6 years old on an outpatient basis.Materials and methods. A prospective analysis of cases of treatment with Umckalor for acute tonsillopharyngitis of non-streptococcal etiology against the background of acute respiratory infection in children 2-6 years old (n = 23, group 1) was carried out.The comparison group (2) consisted of children receiving standard therapy (n = 22). Systemic antibiotics and antiviral drugs were not used in all patients. The study was conducted over 6 months (2019-2020). The research results were processed using the Statistica 7.0 software (StatSoft, USA).Results and discussion. Therapy with Umckalor in children with acute tonsillopharyngitis contributed to the elimination of the main clinical symptoms of the disease on the 4th day of treatment (efficiency was 69.9%, p < 0.05). The most persistent symptom was hyperemia of the tonsils and oropharynx. The period of convalescence occurred 2 days earlier than in the children of group 2 (p < 0.05). Umckalor was well tolerated, no side effects of the drug were identified.Conclusions. The drug Umckalor can be recommended for monotherapy for 7 days in patients with acute non-streptococcal tonsillopharyngitis on an outpatient basis with mild and moderate disease.
The efficacy and safety of the cytomucoprotector gelatin tannate in the treatment of acute gastroenteritis in children
Authors: Meskina E. R. , Ushakova A. Yu. , Khadisova M. K. , Stashko T. V. , Antonenko A. N. 
Published: November 2021

Introduction.Acute gastroenteritis (AGE) is one of the leading causes of pediatric morbidity and mortality. The use of cytomuco-protectors that stabilize the intestinal barrier can increase the effectiveness of treatment.The aimis to determine the gelatin tannate (GT) clinical efficacy, safety and antiviral activity in AGE in children admitted to the hospital.Materials and methods.An open, prospective, randomized study included 52 children <7 years of age with AGE. The intervention group (n = 28) received GT, which was administered orally at 250 mg to children <3 years of age and 500 mg to children £ 3 years of age 4 times a day for 7 days. The severity of AGE (Vizikari scale: 10-14 points and CDC: 1-5 points) in parallel groups was significant. Standard treatment included rehydration and probiotic B. bifidum 1 (15 * 108 CFU / day in three divided doses). Antibiotic therapy (ABT) was received by 50% of children from each group. Fecal samples at screening and on the 7th day of treatment were examined in Real-time PCR with the determination of nucleic acids of rotaviruses A, noro-, adeno-, astro- and enteroviruses. The viral etiology of AGE was confirmed in 53.8%, salmonellosis - in 3.8% and campylobacteriosis - in 1.9% of patients.Results.GT reduced the risk of diarrhea on day 5 by 32% [95% confidence interval 14-50%] OR 0.25 [0.11-0.25] and by 14% [4-24%] by day 7. In the GT group, on day 7 of treatment, all patients had normal stool consistency. GT reduced the time for stool normalization by 1.3 days [0.2-2.3]. A more significant effect was obtained in patients with risk factors for insufficient treatment efficacy and in patients with inflammatory AGE requiring ABT. In those who received GT, a decrease in the concentration of viruses in feces was found, in the control - no statistical differences. The recurrence of AGE symptoms during hospital treatment was recorded with a frequency of 3.6% and 16.7%, respectively (p = 0.111).Conclusions.GT significantly reduced the risks of incomplete therapy effect by day 7 of treatment and the average positiveness of stool normalization. Trends in the antiviral action of GT and its positive effect on the incidence of nosocomial viruses.