bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alfabetização e letramento na educação do campo: Jogos Didáticos e Atividades Contextualizadas com os Saberes Campesinos
Authors: Silva Marcia Batista da , Franco Maria Joselma Do Nascimento , Silva Amós Santos 
Published: April 2021

O presente artigo tomou como objeto “os processos de alfabetização e letramento no contexto campesino” e buscou averiguar as contribuições da ludicidade mediada pelos jogos didáticos no progresso da alfabetização e letramento de crianças do 3º ano do ensino fundamental de uma escola do campo, em 2017. Desenvolvida em uma escola atendida pelo Programa de Bolsa de Iniciação à Docência (PIBID) do curso de Pedagogia na Universidade Federal de Pernambuco (UFPE-CAA), a pesquisa teve como objetivos: i) analisar as contribuições da ludicidade pautada nos jogos didáticos, enquanto mobilizadores da participação dos estudantes nas aulas; e ii) identificar se houve avanço quanto ao desenvolvimento da alfabetização e letramento dos estudantes na medida em que a metodologia adotada envolvia a ludicidade pautada nos jogos didáticos em sala. Foram utilizados como arcabouço teórico para elaboração deste trabalho: Leal, Mendonça, Morais e Lima (2008); Soares (2003); Ferreiro (2004); Ferreiro e Teberosky (1979); Morais (2012); Soares (2010); Coutinho (2005); Caldart (2012); Arroyo (2004); Kishimoto (2002); Murcia (2005); Miranda (2001). A pesquisa é de abordagem qualitativa, tendo como lócus uma escola pública municipal localizada em Caruaru-PE, no território campesino do Alto do Moura. Metodologicamente, trabalhamos com a observação participante e a intervenção pedagógica, cujos dados foram registrados em dois diários de campo. Os participantes da pesquisa foram tratados como E1, E2 e E3. Os resultados mostram que, na avaliação diagnóstica inicial, os três estudantes (E1, E2 e E3) se apresentavam na fase silábico-alfabético do desenvolvimento do sistema de escrita alfabética (SEA), porém, no decorrer das atividades que envolviam a ludicidade articulada aos jogos didáticos, evidenciou-se que os estudantes avançaram para o nível alfabético do sistema de escrita, confirmado na avaliação diagnóstica final. Concluiu-se que o caminho metodológico construído, envolvendo a ludicidade mediada pelos jogos didáticos, materializou-se enquanto possibilidade de atuação dos iniciantes à docência do PIBID, marcado pelos avanços que os estudantes apresentaram no percurso construído, desencadeando satisfação na participação das aulas, tendo como consequência os avanços nos processos de construção da alfabetização e letramento pelos estudantes.
Uma metáfora para describir el aprendizaje con base en la física cuántica
Authors: Retana José Ángel García , González Greivin Hernández 
Published: April 2021

El aprendizaje es un proceso complejo, en el que intervienen y se vinculan múltiples variables. La construcción del aprendizaje es un acto individual que se desarrolla en un contexto natural y social. Ninguno de estos aspectos se puede considerar como nuevo, sin embargo el visualizar el aprendizaje y todas esas características tomando en cuenta la dinámica de las partículas propuesta desde la perspectiva de la Física cuántica, nos ofrece un nuevo modelo para reflexionar sobre este aspectos.
Estilos de aprendizagem e intenção empreendedora: o caso do curso técnico em administração
Authors: Abreu Arlete Aparecida , Peres Júnior Miguel Rivera , Fernandes Cássia do Carmo Pires 
Published: April 2021

O objetivo desta pesquisa é identificar se há correlação entre o estilo de aprendizagem, baseado no modelo de Felder; Silverman (1988) e a intenção em empreender, baseada no modelo de Liñan e Chen (2009). O objeto foram 36 alunos da disciplina de Empreendedorismo do curso técnico em Administração do Instituto Federal de Minas Gerais – Campus Formiga. Em relação à intenção empreendedora, destaca-se o papel das variáveis relacionadas à percepção e atitudes pessoais sobre o tema. É importante afirmar que não há correlação estatística significativa entre o estilo de aprendizagem e a intenção empreendedora; no entanto, é preciso atenção aos estilos de aprendizagem, adotando metodologias adequadas a características dos alunos, com o objetivo de fortalecer a intenção empreendedora.
Conformational features and ionization states of Lys side chains in a protein studied using the stereo-array isotope labeling (SAIL) method
Authors: Takeda Mitsuhiro , Miyanoiri Yohei , Terauchi Tsutomu , Kainosho Masatsune 
Published: April 2021

Abstract. Although both the hydrophobic aliphatic chain and hydrophilic ζ-amino group of the Lys side
chain presumably contribute to the structures and functions of proteins, the
dual nature of the Lys residue has not been fully investigated using NMR
spectroscopy, due to the lack of appropriate methods to acquire
comprehensive information on its long consecutive methylene chain. We
describe herein a robust strategy to address the current situation, using
various isotope-aided NMR technologies. The feasibility of our approach is
demonstrated for the Δ+PHS/V66K variant of staphylococcal nuclease (SNase),
which contains 21 Lys residues, including the engineered Lys-66 with an
unusually low pKa of ∼ 5.6. All of the NMR signals for
the 21 Lys residues were sequentially and stereospecifically assigned
using the stereo-array isotope-labeled Lys (SAIL-Lys), [U-13C,15N;
β2,γ2,δ2,ε3-D4]-Lys. The complete set of assigned 1H, 13C, and
15N NMR signals for the Lys side-chain moieties affords useful
structural information. For example, the set includes the characteristic
chemical shifts for the 13Cδ, 13Cε, and
15Nζ signals for Lys-66, which has the deprotonated ζ-amino group, and the large upfield shifts for the 1H and 13C
signals for the Lys-9, Lys-28, Lys-84, Lys-110, and Lys-133 side chains, which are
indicative of nearby aromatic rings. The 13Cε and
15Nζ chemical shifts of the SNase variant selectively labeled
with either [ε-13C;ε,ε-D2]-Lys or SAIL-Lys, dissolved in H2O and D2O, showed that
the deuterium-induced shifts for Lys-66 were substantially different from
those of the other 20 Lys residues. Namely, the deuterium-induced shifts of
the 13Cε and 15Nζ signals depend on the
ionization states of the ζ-amino group, i.e., −0.32 ppm for Δδ13Cε [NζD3+-NζH3+] vs. −0.21 ppm for Δδ13Cε [NζD2-NζH2] and −1.1 ppm for Δδ15Nζ[NζD3+-NζH3+] vs. −1.8 ppm for Δδ15Nζ[NζD2-NζH2]. Since the 1D 13C NMR spectrum of a
protein selectively labeled with [ε-13C;ε,ε-D2]-Lys shows narrow (> 2 Hz) and
well-dispersed 13C signals, the deuterium-induced shift difference of
0.11 ppm for the protonated and deprotonated ζ-amino groups, which
corresponds to 16.5 Hz at a field strength of 14 T (150 MHz for
13C), could be accurately measured. Although the isotope shift
difference itself may not be absolutely decisive to distinguish the
ionization state of the ζ-amino group, the 13Cδ,
13Cε, and 15Nζ signals for a Lys residue
with a deprotonated ζ-amino group are likely to exhibit distinctive
chemical shifts as compared to the normal residues with protonated ζ-amino groups. Therefore, the isotope shifts would provide a useful
auxiliary index for identifying Lys residues with deprotonated ζ-amino groups at physiological pH levels.
The Role of Public Schooling in Preparing Youth for Civic Reasoning and Engagement
Authors: Lee Carol D. 
Published: April 2021

Abstract
This article explores the role of public schooling in preparing youth to engage in civic reasoning and civic action. The current challenges in the public arena—a pandemic, economic depression, civil unrest over continued systemic racism—illustrate the complexity of sense-making required to address these conundrums. At the same time, these challenges highlight inequities that have persisted over the course of U.S. history. The article further explores the mix of challenges and opportunities that the structure of the U.S. government poses to citizens hoping to grapple with these complexities, arguing that the knowledge base required to meet these civic challenges and opportunities are not just cognitive but epistemological and ethical. The article concludes that the development of such a complex knowledge base must be distributed across the K–12 public education sector: not limited to civics classes, but distributed across all the disciplines taught in schools.
Campus No.1
Authors: Editor El 
Published: April 2021

It contains: I Congress of Human Resources. Twelfth Century Management: in response to current problems ; X Congress of Business Administration and Marketing Students: The perfect Match, TICS and millennials ; Courses and diplomas: Department of Business Administration, Department of Accounting and Finance ; Chair of Accounting and Auditing.
Realidad Empresarial No.1, 2016: 29-34
Expression of toll-like receptor 4 gene polymorphism and inflammatory factors in peripheral blood on patients with sepsis
Authors: Tian Tieying , Xu Xiyuan , Yang Jingping 
Published: April 2021

Objective: To observe the presence of toll-like receptor 4 (TLR4) gene polymorphism in the venous blood in patients with sepsis, meanwhile, to observe the expression and the diagnostic value of TLR4 mRNA, interferon-γ (IFN-γ), interleukin-23 (IL-23), procalcitonin (PCT) and C-reactive protein (CRP) in patients with different degrees of sepsis.Methods: The peripheral blood samples from the subjects were collected to extract genomic DNA, gene sequencing and enzyme digestion method were applied to the detection of TLR4 Asp299Gly loci polymorphism. The expression of TLR4 mRNA in the peripheral blood was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The expression levels of IFN-γ, IL-23, PCT and CRP were measured by enzyme linked immunosorbent assay (ELISA).Results: (1) The gene polymorphism was not present in TLR4 Asp299Gly loci; (2) The expression of TLR4 mRNA in the peripheral blood in patients with sepsis: on d1, there were statistically significant differences between the normal group and the group (APACHE II ≤ 20), between the normal group and the group (APACHE II > 20), between the group (APACHE II ≤ 20) and the group (AAPACHE II > 20) (t = 5.741, 14.780 and 10.500, all p < .01). APACHE II stands for Acute Physiology and Chronic Health Evaluation II. On d7, there were statistically significant differences between the normal group and the group (APACHE II ≤ 20), between the normal group and the group (APACHE II > 20), between the group (APACHE II ≤ 20) and the group (APACHE II > 20) (t = 4.186, 13.830 and 9.645, all p < .01); (3) The expression levels of IFN-γ, IL-23, PCT and CRP were obviously upregulated (all p < .01), and TLR4 was positively correlated with IFN-γ and IL-23 (all p < .01); (4) The best cutoff value of TLR4 mRNA at baseline was 891.6 μg/L, with a sensitivity of 100% and a diagnostic specificity of 57%. The best cutoff value of IFN-γ at baseline was 84.5 μg/L, with a sensitivity of 100% and a diagnostic specificity of 57%. The best cutoff value of IL-23 at baseline was 861 μg/L, with a sensitivity of 100% and a diagnostic specificity of 97%.Conclusions: (1) The Asp299Gly polymorphism is not present in TLR4 gene; (2) The expression levels of TLR4 mRNA, IFN-γ and IL-23 are relatively high in patients with sepsis, and will be higher with the increased severity of sepsis; (3) TLR4 mRNA, IFN-γ and IL-23 can be used as molecular biomarkers for the early diagnosis of sepsis.
Mechanical and Microstructural Properties of Copper Slag Based Blended Geopolymer Concrete
Authors: RATHANASALAM Vijayasarathy , PERUMALSAMI Jayabalan , JAYAKUMAR Karthikeyan 
Published: April 2021

This work presents a novel way to examine the characteristics of fly ash, copper slag (CPS) along with the addition of Ultrafine Ground Granulated Blast Furnace Slag (UFGGBFS) based Geopolymer Concrete (GPC) for various molarities (10M, 12M and 14M). In GPC, fly ash was replaced with UFGGBFS (5 %, 10 % and 15 %) and copper slag was used as fine aggregate. Mechanical Characterization such as split tensile, flexural strength, workability and water absorption were conducted . GPC characterization and microstructural behaviour was studied by examining X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). From experimental results this study concludes that with a rise in molarity of GPC, along with incorporation of UFGGBFS, improved the performance, densification and strength of GPC.
Synthesis of Lauric-Myristic Acid/Activated Carbon Composite as a New Shape-Stabilized Energy Storage Material
Authors: Chen Yanghua , GE Minrong , ZHAO Feng , YANG Xue 
Published: April 2021

In this work, a new composite phase change material (CPCM) with lauric-myristic acid (LA-MA) eutectic as PCM and activated carbon (AC) was used as supporting material with four different mass ratios of 5.0:5.0, 5.5:4.5, 6.0:4.0, and 6.5:3.5, respectively. The properties and microstructure of LA-MA/AC were analyzed by some characterization methods. The results show that the composite process of LA-MA eutectic and AC was a simple physical mixing and no new chemical bonds were found. The fusion and freeze temperature, enthalpy of the samples were measured by differential scanning calorimetry (DSC), and the residual weight of the samples was analyzed by thermogravimeter (TGA). It was shown that the fusion and freeze temperature of LA-MA eutectic separately were 32.42 ℃ and 33.63 ℃, and its fusion enthalpy and freeze enthalpy were 152.64 J/g and 148.8 J/g, respectively. TGA data shows that the thermal stability of LA-MA eutectic was obviously improved by adding AC as a support material. The results of this study can be available for reference to solar energy storage applications.
Nanostructured VO2 (A) and VO2 (M) Derived from VO2 (B): Facile Preparations and Analyses of Structural, Thermal, Optical and Thermophysical Properties
Authors: BARRA Hamdi Muhyuddin , CHEN Soo Kien , TAMCHEK Nizam , TALIB Zainal Abidin , LEE Oon Jew , TAN Kar Ban 
Published: April 2021

Vanadium dioxide (VO2) is an interesting compound that exists in different polymorphic phases with varying characteristics and potential applications. In this study, fabrication and property analyses of three VO2 polymorphs, namely VO2 (B), VO2 (A), and VO2 (M), are described. Specifically, VO2 (B) was prepared via hydrothermal method under a low synthesis temperature of 180 °C and a fast processing time of 24 h. In addition, VO2 (A) and VO2 (M) were derived from the as-synthesized VO2 (B) using sequential hydrothermal treatment and calcination process, respectively. From the field-emission scanning electron microscope (FESEM) scans, belt-like VO2 (B) nanoparticles with dimensions of as low as 35 × 130 nm2 were formed. Further, hydrothermal treatment of the as-synthesized VO2 (B) resulted to a flower-shaped VO2 (A) due possibly to an oriented attachment mechanism. Meanwhile, annealing of the VO2 (B) sample caused granular growth that produced plate-like and oblate shaped VO2 (M) with dimensions of 135 to 306 nm in diameter and 28 to 37 nm in thickness. Moreover, the thermochromic properties of the samples were examined using differential scanning calorimetry (DSC) while the thermophysical properties of the samples were measured via microflash method. Accordingly, the prepared VO2 (A) and VO2 (M) samples exhibited phase transition behavior at 168.37 °C and 68.6 °C, respectively. Subsequently, changes in the thermophysical properties of each sample can be observed across the measured transition temperature. In particular, an increase in both the thermal diffusivity and thermal conductivity of VO2 (M) can be observed when the temperature was raised from 50 °C to 100 °C. On the other hand, there is a noticeable decrease in the thermal conductivity of VO2 (A) when the temperature was increased from 150 °C to 200 °C.
Silver-containing Cation Exchange Resin: Synthesis and Application
Authors: KHAYDAROV Renat , ABDUKHAKIMOV Murodjon , GARIPOV Ilnur , SADIKOV Ilkham , KRISHNAMURTHY Praveen Thaggikuppe , EVGRAFOVA Svetlana 
Published: April 2021

Cation exchange resins are widely used for water softening and demineralization all over the world. Deposition, metabolism, and growth of bacteria and fungi on the resin beads cause capacity and performance losses, especially during repeated use in cyclic and long-term operations. Over the last decades, modification of different materials by silver nanoparticles (AgNPs) has demonstrated to present significant opportunities in mitigating biofouling problems. The paper deals with a novel facile technique of introducing silver colloids (AgC) into cation exchange resin, providing the formation of silver micro- and nano-inclusions on the cation resin beads. The scanning electron microscope (SEM) measurements have confirmed a spherical shape and uniform distribution of AgC (50 – 1000 nm) on the surface of the resin. To evaluate the antibacterial and fungicidal properties of AgC on the cation resin beads, we have used Aureobasidium sp., Penicillium sp., and Staphylococcus aureus cultures. AgC coating has proved to efficiently prevent bacteria/biofilm growth on the cation resin beads and thereby significantly increase the service life of the cation exchange resin, especially in hot climatic conditions. Possible antibiofouling mechanisms of the modified nanocomposite cationite have been discussed. Since 2020, the modified silver-containing cationite has been successfully utilized for water softening systems of boiler equipment in Uzbekistan, demonstrating the suitability of the suggested facile coating technique for reducing fouling of cation-exchange resin.
Integrated metabolome and transcriptome revealed the flavonoid biosynthetic pathway in developing Vernonia amygdalina leaves
Authors: Shui Lanya , Huo Kaisen , Chen Yan , Zhang Zilin , Li Yanfang , Niu Jun 
Published: April 2021

Background
Vernonia amygdalina as a tropical horticultural crop has been widely used for medicinal herb, feed, and vegetable. Recently, increasing studies revealed that this species possesses multiple pharmacological properties. Notably, V. amygdalina leaves possess an abundance of flavonoids, but the specific profiles of flavonoids and the mechanisms of fl avonoid bi osynthesis in developing leaves are largely unknown.

Methods
The total flavonoids of V. amygdalina leaves were detected using ultraviolet spectrophotometer. The temporal flavonoid profiles of V. amygdalina leaves were analyzed by LC-MS. The transcriptome analysis of V. amygdalina leaves was performed by Illumina sequencing. Functional annotation and differential expression analysis of V. amygdalina genes were performed by Blast2GO v2.3.5 and RSEM v1.2.31, respectively. qRT-PCR analysis was used to verify the gene expressions in developing V. amygdalina leaves.

Results
By LC-MS analysis, three substrates (p-coumaric acid, trans-cinnamic acid, and phenylalanine) for flavonoid biosynthesis were identified in V. amygdalina leaves. Additionally, 42 flavonoids were identified from V. amygdalina leaves, including six dihydroflavones, 14 flavones, eight isoflavones, nine flavonols, two xanthones, one chalcone, one cyanidin, and one dihydroflavonol. Glycosylation and methylation were common at the hydroxy group of C3, C7, and C4’ positions. Moreover, dynamic patterns of different flavonoids showed diversity. By Illumina sequencing, the obtained over 200 million valid reads were assembled into 60,422 genes. Blast analysis indicated that 31,872 genes were annotated at least in one of public databases. Greatly increasing molecular resources makes up for the lack of gene information in V. amygdalina. By digital expression profiling and qRT-PCR, we specifically characterized some key enzymes, such as Va-PAL1, Va-PAL4, Va-C4H1, Va-4CL3, Va-ACC1, Va-CHS1, Va-CHI, Va-FNSII, and Va-IFS3, involved in flavonoid biosynthesis. Importantly, integrated metabolome and transcriptome data of V. amygdalina leaves, we systematically constructed a flavonoid biosynthetic pathway with regards to material supplying, flavonoid scaffold biosynthesis, and flavonoid modifications. Our findings contribute significantly to understand the underlying mechanisms of flavonoid biosynthesis in V. amygdalina leaves, and also provide valuable information for potential metabolic engineering.
Flexible Self‐Powered Integrated Sensing System with 3D Periodic Ordered Black Phosphorus@MXene Thin‐Films
Authors: Zhang Yupu , Wang Lili , Zhao Lianjia , Wang Kang , Zheng Yiqiang , Yuan Zeyu , Wang Dongyi , Fu Xiyao , Shen Guozhen , Han Wei 
Published: April 2021

AbstractAccurate and continuous detection of physiological signals without the need for an external power supply is a key technology for realizing wearable electronics as next‐generation biomedical devices. Herein, it is shown that a MXene/black phosphorus (BP)‐based self‐powered smart sensor system can be designed by integrating a flexible pressure sensor with direct‐laser‐writing micro‐supercapacitors and solar cells. Using a layer‐by‐layer (LbL) self‐assembly process to form a periodic interleaving MXene/BP lamellar structure results in a high energy‐storage capacity in a direct‐laser‐writing micro‐supercapacitor to drive the operation of sensors and compensate the intermittency of light illumination. Meanwhile, with MXene/BP as the sensitive layer in a flexible pressure sensor, the pressure sensitivity of the device can be improved to 77.61 kPa–1 at an optimized elastic modulus of 0.45 MPa. Furthermore, the smart sensor system with fast response time (10.9 ms) shows a real‐time detection capability for the state of the human heart under physiological conditions. It is believed that the proposed study based on the design and integration of MXene materials will provide a general platform for next‐generation self‐powered electronics.
A New Porous Metallic Carbon Allotrope with Interlocking Pentagons for Sodium‐Ion Battery Anode Material
Authors: Ni Dongyuan , Guo Yaguang , Shen Yupeng , Wang Qian 
Published: April 2021

AbstractThe high rate performance of metal‐ion batteries requires the anode to be conductive not just ionically but also electronically. This criterion has significantly stimulated study on porous metallic carbon with intrinsically high electron conductivity. In contrast to the previously reported metallic T6‐carbon constructed by interlocking carbon hexagons, here a new 3D metallic carbon composed of interlocking carbon pentagons, penta‐h22, is proposed. Based on state‐of‐the‐art theoretical calculations, it is shown that penta‐h22 is energetically more stable than T6‐carbon. More importantly, different from T6‐carbon with too small pores for metal ions, penta‐h22 possesses a large specific capacity of 609 mAh g–1, low diffusion barrier of 0.20 eV, low open‐circuit voltage of 0.63 V, and small volume change of 1.75%. These parameters clearly indicate that pentagon‐based 3D porous carbon is promising for the anode of Na‐ion batteries.