bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti Bacterial Activity of Lavandula Extracts, Pathogen and Probiotics Bacteria
Authors: khodagholi Asal , Soltani Mahdieh , Akbarin Mohammad Mehdi , Farjami Zahra 
Published: July 2021

Abstract
Introduction: Herbs of the lavender genus are widely used today as anti-bacterial drugs in traditional medicine. Lavandula angustifolia (LA) is endemic in the Iberian Peninsula and common in Iran that belongs to the Lamiaceae family. Lavandula oils, well known for their scent and aroma, have been used in the perfumery and food industry for many years, therefore the aim of this study is the assessment of the anti-bacterial effect of LA extracts against pathogens and non-pathogen bacteria. Methods: 96-well microplates MICs were determined by the broth microdilution method. Five Serial dilutions from 50 to 1 μg/mL concentrations were admitted for all bacteria which include: Escherichia Coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and Probiotics complex. Results: LA watery extract demonstrate the statically inhibitory effect in just 50 g/L concentration against E.Coli, E. Faecalis, Pu, and Probiotic p= 0.024, 0.025, 0.004, and 0.012 respectively, whereas this concentration was 1g/L for Staph. aureus p=0.026. LA alcohol extracts display the same effect in 1g/L for all bacteria p= 0.000.Conclusion: Our results showed that LA in both watery and Alcohol extractions can inhibit both pathogenic and non-pathogen bacteria whereas active compounds are alcohol soluble. Long-time consumption of LA in the herbal product could disrupt normal bacteria of the gastrointestinal system.
The Process Development of Glass Cullet and Recycled Glass Aggregate for Improving Recycling Rate
Authors: Lee Hansol , Lee Hoon 
Published: July 2021

Abstract
Due to depletion of resources and the spread of environmental pollution, the sustainability of raw materials is emerging as an important issue. Glass bottles are one of the products that are easy to recycle, and many studies have been conducted to improve the recycling rate. In this study, we attempted to develop the waste glass bottles process that can be recycled into a glass cullet and recycled glass aggregate. To produce a cullet from waste glass bottles(WGBs), color quality standards must be satisfied. Therefore, we applied a multistage color sorter to the experiment. The recycled aggregate glass must adjust the particle size. Thus, we experimented with the optimum crusher selection test when applying the crushing process. And, we confirmed the appropriateness of process by aspect ratio analysis of product. In addition, we confirmed the trends in the data required to set the optimum design and operating conditions of the selected vertical shaft impact(VSI) crusher using discrete element method(DEM) simulations.
LncRNA EGFR-AS1 Regulates Breast Cancer Cells Function and Sensitivity to Docetaxel Via the miR-149-5p/ELP5 Axis
Authors: Li Shiping , Mi Xiaoyi , Sun Mingfang , Zhang Jie , Hao Miaomiao , Cao Qixue , Diao Kexin , Wang Jian , Li Hanwen , Liu Bin 
Published: July 2021

Abstract
Background: Recently, an increasing number of studies have focused on investigating long non-coding RNAs (lncRNAs) and their role in regulating the progression of various cancer types. However, the biological effects and underlying mechanisms of EGFR-AS1, a typical lncRNA, remain largely unclear in breast cancer.Methods: Differential expression of EGFR-AS1 in breast cancer tissue was analyzed using an integrative database and verified in breast cancer tissue samples and cells via real-time PCR analysis and western blotting analysis. The tumor promoter role of EGFR-AS1 in breast cancer cells was determined through MTT, EDU analysis, colony formation and transwell assays,and the effect of EGFR-AS1 on docetaxel drug sensitivity was examined. We then performed bioinformatic analysis and the dual-luciferase reporter assay to identify the binding sites of EGFR-AS1/miR-149-5p and miR-149-5p/ELP5. Results from western blotting and biological function studies provided insights into whether the EGFR-AS1/miR-149-5p/ELP5 axis regulates breast cancer development in vitro and in vivo. Results: EGFR-AS1 is upregulated in breast cancer tissues and cells and promotes the progression of breast cancer cells both in vitro and in vivo. Moreover, miR-149-5p is downregulated in breast cancer tissues and cell lines. Mechanistically, EGFR-AS1 regulates ELP5 levels by sponging miR-149-5p, thereby affecting cell progression and promoting epithelial-to-mesenchymal transition. Hence, the EGFR-AS1/miR-149-5p/ELP5 axis is involved in breast cancer proliferation, migration, invasion, and resistance to the chemotherapeutic drug, docetaxel, in breast cancer cells. Conclusions: EGFR-AS1 sponges miR-149-5p to affect the expression level of ELP5 ultimately acting as a new tumor promotor in breast cancer. This study provides novel insights into diagnostic and docetaxel-related chemotherapy targets for breast cancer.
Critical Evaluation of the Agronomic Effectiveness of Recovered Phosphate Fertilizer Produced From Incinerated Sewage Sludge Ash
Authors: FANG Le , Wang Qiming , Li Jiang-shan , Poon Chi Sun 
Published: July 2021

Abstract
Phosphorus (P) recovery from incinerated sewage sludge ash (ISSA) has been extensively investigated, and various recovered phosphate fertilizers (RPFs) have been produced. In this study, three RPFs (calcium phosphate compounds (CaP), struvite (SP), and P-loaded biochar (BP)) produced from ISSA were characterized and their agronomic effectiveness were verified by pot experiments with the cultivation of choy sum (Brassica campestris L. ssp. Chinensis var. utillis Tsen et Lee) and ryegrass (Lolium perenne L.). The SP has the highest P purity while the BP has the most complex P species. And the plant growth results showed that the RPFs greatly facilitated plant growth and demonstrated superior/comparable effects to those of MP/CoF. In general, choy sum fertilized with SP showed the best effect due to the Mg involved and the high P purity of SP, while ryegrass fertilized with BP performed the best among all of the groups because of the additional nutritional elements and the high P availability of BP. Additionally, the accumulation of heavy metals in the plants under all conditions did not exceed the limits stipulated in the regulations. These results indicate that recovering P from ISSA is an attractive technology to produce P fertilizers, which can alleviate both the scarcity of phosphate resources and the burden of ISSA management.
Protective role of mRNA demethylase FTO on axon guiding molecules of nigro-striatal projection system in manganese-induced Parkinsonism
Authors: Qi Zhipeng , Li Jiashuo , Zhang Ke , Liu Yanan , Yang Xinxin , Li Minghui , Du Xianchao , Zhang Lei , Wen Yi , Xu Bin , Liu Wei , Xu Zhaofa , Deng Yu 
Published: July 2021

Abstract
Background

Parkinson's disease (PD) is a neurodegenerative disease caused by environmental and genetic factors. Manganese (Mn) exposure is a major environmental cause of PD. Cellular and molecular mechanism of Parkinsonism caused by Mn has not been explored clearly. In addition, patients with Mn-induced Parkinsonism show poor therapeutic response to levodopa. Therefore, there is need to explore the mechanisms underlying neurotoxicity of Mn exposure.
Methods

In short, we used SH-SY5Y cells and C57BL/6 mice to characterize Mn-induced Parkinsonism. We measured the behavioral, histological, ultrastructural and nigro-striatal projection system changes, cell viability, axon growth, and other target indicator levels, which led to the discovery of a novel mechanism of Mn-induced neurotoxicity.
Results

The findings of the current study showed that inhibition of fat mass and obesity-associated protein (FTO)-mediated demethylation of N6-methyladenosine (m6A) mRNA aggravates Mn-induced motor dysfunction. Notably, FTO level is low in Mn exposure model mice and enhances occurrence of dyskinesia in mice. Over-expression of FTO reduces m6A methylation in the key axon guiding molecules of nigro-striatal projection system, including ephrin-A5 and ephrin-B2. It increases ephrin-B2 mRNA decay through the m6A reader YT521-B homology domain family proteins 2 (YTHDF2).
Conclusions

The findings of this study show that FTO, a m6A demethylase, performs an indispensable function in Mn-induced Parkinsonism. Notably, re-expression of FTO and ephrin-B2 improved motor dysfunction after Mn exposure.
Quality of knee osteoarthritis care in the Netherlands: A survey on the perspective of people with osteoarthritis
Authors: Oomen J.M.H. , Peters Y.A.S. , Ende C.H. van den , Schers H.J. , Assendelft W.J.J. , Vriezekolk J.E. , Koëter S. 
Published: July 2021

Abstract
Background: Quality indicators (QIs) are used to monitor quality of care and adherence to osteoarthritis (OA) standards of care. Patient reported QIs can identify the most important gaps in quality of care and the most vulnerable patient groups. The aim of this study was to capture the perspective of people with knee OA (KOA) in the Netherlands on the quality of care received, and explore determinants related to lower achievement rates.Methods: We sent an online survey to all members of The Dutch Knee Panel (n = 622) of the Sint Maartenskliniek Nijmegen, the Netherlands. The survey consisted of a slightly adapted version of the “OsteoArthritis Quality Indicator” (OA-QI) questionnaire (18 items; yes, no, N/A); a rating of quality of KOA care on a 10-point scale; a question on whether or not one wanted to see change in the care for KOA; and an open-ended question asking recommendations for improvement of OA care. Furthermore, sociodemographic and disease related characteristics were collected. Pass rates for separate QIs and pass rates on patient level were calculated by dividing the number of times the indicator was achieved by the number of eligible persons for that particular indicator. Results: A total of 434 participants (70%) completed the survey. The mean (SD) pass rate for separate QIs was 49% (20%); ranging from 15% for receiving referral for weight reduction to 75% for patient education on how to manage knee OA. The mean (SD) pass rate on patient level was 52% (23%). Presence of OA in other joints, comorbidities, and having a knee replacement were associated with higher pass rates. On average, a score of 6.5 (1.6) was given for the quality of care received, and the majority of respondents (59%) wanted change in the care for KOA. Of 231 recommendations made, most often mentioned were the need for tailoring of care (14%), more education (13%), and more empathy and support from healthcare providers (12%).Conclusion: This study found patients are only moderately satisfied with the OA care received, and showed substantial gaps between perceived quality of care for OA and internationally accepted standards. Future research should focus on the underlying reasons and provide strategies to bridge these gaps.
Hepatic Metastasis from Perianal Paget's Disease Without Identified Underlying Carcinoma: A Case Report
Authors: Cao Yi-Sheng , Wang Shu-Yan 
Published: July 2021

Abstract
Background: Perianal Paget’s disease (PPD) is a rare malignancy, often associated with an underlying adenocarcinoma and a poor prognosis. Case presentation: A 69-year-old female was presented with a history of perianal pruritus for 6 months and enlarged inguinal lymph nodes in the left side. Paget cells were confirmed by pathology after a wide excision of perianal skin. Radiotherapy was performed covering the bilateral inguinal lymphatic drainage area. Hepatic metastasis was found 8 months after surgery. Hepatic artery embolization (HAE) and high intensity focused ultrasound therapy (HIFU) were performed successively. However, hepatic metastasis happened again 3 months later. Ultrasound guided percutaneous radiofrequency ablation (PRFA) was carried out and various means of inspection could not identify the primary tumor. In the case of rapid progression of the tumor, we gave the patient chemotherapy regimens of XELOX. After four cycles’ chemotherapy, the tumor marker went down continuously and the hepatic metastasis stayed stable. Conclusions: Hepatic metastasis from perianal Paget's disease without identified underlying carcinoma may benefit from XELOX on the basis of adenocarcinoma.
Centralized colorectal cancer screening outreach and patient navigation for vulnerable populations in North Carolina: Study protocol for the SCORE randomized controlled trial
Authors: Malo Teri L. , Correa Sara Y. , Moore Alexis A. , Ferrari Renée M. , Leeman Jennifer , Brenner Alison T. , Wheeler Stephanie B. , Tan Xianming , Reuland Daniel , Consortium SCORE Study Protocol 
Published: July 2021

Abstract
BackgroundAlthough colorectal cancer (CRC) screening is effective in reducing CRC mortality, screening rates in vulnerable populations served by community health centers (CHCs) remain below national targets. CHCs in North Carolina are challenged to reach CRC screening targets as they tend to be under-resourced, have limited capacity to implement and sustain population health interventions, and typically operate independently from one another and from regional colonoscopy providers. The Scaling Colorectal Cancer Screening Through Outreach, Referral, and Engagement (SCORE) project is designed to address barriers to CRC screening in partnership with CHCs by implementing a multilevel intervention that includes centralized support infrastructure for mailed fecal immunochemical test (FIT) outreach and patient navigation to follow-up colonoscopy. This paper describes protocols for the SCORE implementation trial.MethodsWe will conduct a type 2 hybrid effectiveness-implementation trial that will assess effectiveness at increasing CRC screening and follow-up rates while also assessing implementation outcomes. The planned trial sample will include 4,000 CHC patients who are at average CRC risk and due for screening. Participants will be randomized 1:1 to receive either usual care or a multilevel intervention that includes mailed FIT outreach and patient navigation support to follow-up colonoscopy for those with abnormal FIT. The primary effectiveness outcome is completion of any CRC screening test at six months after randomization. We will also conduct a multilevel assessment of implementation outcomes and determinants.DiscussionThis hybrid effectiveness-implementation trial will evaluate the effectiveness and implementation of an intervention that provides centralized infrastructure for mailed FIT screening and patient navigation for CHCs that operate independently of other healthcare facilities. Findings from this research will enhance understanding of the effectiveness of a centralized approach and factors that determine successful implementation in vulnerable patient populations.Trial registrationThe trial was registered on May 28, 2020 at ClinicalTrials.gov (identifier NCT04406714).
Spatial Extent of Amyloid-β Levels and Associations with Alzheimer’s Disease Biomarkers
Authors: Ozlen Hazal , Binette Alexa Pichet , Köbe Theresa , Meyer Pierre-Francois , Breitner John , Poirier Judes , Villeneuve Sylvia 
Published: July 2021

Abstract
Objective: To investigate the biological and clinical correlates of Aβ spatial extent deposition levels in cognitively unimpaired older adults.Methods: We included cognitively unimpaired older adults from three cohorts, totalling 529 participants (PREVENT-AD, n=129; ADNI, n=400 and HABS, n=288) who underwent Aβ positron emission tomography (PET). We used Gaussian-mixture models to identify region-specific thresholds of Aβ positivity in seven brain regions prone to early Aβ accumulation. Individuals were classified as having “widespread” Aβ deposition if they were positive in all seven regions, “regional” Aβ deposition if they were positive in one to six regions, or Aβ negative if negative in all regions. We compared demographics, genetics, tau-PET binding, and cognitive performance and decline between the three groups.Results: In all cohorts, most participants with regional Aβ-PET binding did not meet the cohort-specific criteria for Aβ-positivity (79% for PREVENT-AD, 57% for ADNI, and 100% for HABS). Regional Aβ groups had normal baseline cognition and relatively normal tau-PET binding, but a greater proportion of APOE ε4 carriers, decreased CSF Aβ1-42 levels, and greater amount of longitudinal Aβ-PET binding accumulation (only available in ADNI and HABS) when compared with the Negative Aβ groups. Widespread Aβ groups had lower baseline cognitive performance (PREVENT-AD only), faster cognitive decline (all cohorts) and greater amount of longitudinal tau binding than the other groups (only available in ADNI and HABS). Conclusions: Individuals with regional Aβ deposition might be the best candidate for preventive trials since they do not yet have widespread tau and cognitive decline. Widespread levels of Aβ seem to be needed for tau spreading.
Utilization of Fecal Occult Blood Testing and Its Impact on Endoscopic Procedures in Hospitalized Patients
Authors: Kumar Vinod , Shahani Shobha , Jaydev FNU , Khatri Jaikumar , Kara Areeba 
Published: July 2021

Abstract
Objective: To gain insights on the impact of fecal occult blood tests, we evaluated inpatients on whom these tests were performed.Patients and Methods: This single center, retrospective study was conducted at a large, academic, tertiary care center. Between Jan 1, 2016-Dec 31, 2017, inpatients who developed a drop in hemoglobin ≥2 grams/dL and had an FOBT were identified. Further data was extracted on a random selection of half of these patients. Patients were categorized as having an overt GI bleed (symptoms of melena, hematochezia, or hematemesis) or not.Results: Over the study period 6,310 patients developed a hemoglobin drop of ≥2 grams/dL. Of these 817 (12.9%) had an FOBT and we reviewed 407 (49.8%) randomly selected patients from this group. Those with missing FOBT results (n=13) were excluded, leaving 394 included in the final analysis. The mean age was 62.7 years with 211 females (53.7%). FOBTs were performed in 34.6% of patients despite the presence of overt GI bleeding. In patients without overt GI bleeding, the proportion of patients who underwent endoscopic procedures was higher in those with a positive FOBT than those with negative FOBT (40.4% vs 13.2%, p < .00001). There were no differences in rates of endoscopic evaluation in patients with overt GI bleeding based on FOBT results.Conclusions: FOBTs continue to be utilized in inpatient settings including in those presenting with overt GI bleeding. In the absence of overt GI bleeding, positive results may drive endoscopic evaluation.
Frost Avoidance: Sodium Alginate + CaCl2 Can Postpone Flowering of ‘Kawanakajima Hakuto’ Peach Trees
Authors: Park Yeonju , Shin Hyunsuk 
Published: July 2021

Abstract
The objective of this study was to investigate whether treatment with sodium alginate and CaCl2 treatment in the 2nd (swollen bud) blooming stage could delay peach flowers as a strategy to avoid frost. Regarding the expression rate at the 3rd (calyx green) stage, the control treatment (CT, distilled water) showed a sharp increase from 8 days after treatments (DAT), reaching the maximum at 14 DAT. However, the 5AG group (5% sodium alginate + 100 mM CaCl2) and the 7AG group (7% sodium alginate + 100 mM CaCl2) recorded the maximum expression rate at 17 DAT. After the maximum expression, CT showed a sharply diminishing trend, whereas 5AG and 7AG showed gentle decreases. At the 4th (calyx red) stage, CT, 5AG, and 7AG reached the peak expression at 20 DAT (43.5%, 31.9%, and 40.2%, respectively). However, maximum expression levels of all treatments were below ~50%. The 5th (first pink) stage was first expressed in all treatments when the expression rate at the 4th stage peaked in all groups at 20 DAT. Maximum expression was 50.7% in CT, 33.7% in 5AG, and 49.2% in 7AG. This tendency was similar to that at the 4th stage. Particularly, expression in 5AG diminished more slowly than that in CT and 7AG at 3rd, 4th, and 5th stages. CT group bloomed first at 20 DAT, reaching full bloom at 81.4%, ahead of AG treatments at 28 DAT, while 7AG and 5AG groups bloomed fully at 34 and 37 DAT, respectively. Our results suggest that 5AG treatment is suitable to delay peach flowering when phenological development of the flower buds reaches the 2nd stage.
Tauber antegrade sclerotherapy: the importance of phlebography in choosing the correct vein.
Authors: Guida Edoardo , Duci Miriam , Boscarelli Alessandro , Ceschiutti Giulia , Schleef Jurgen 
Published: July 2021

Abstract
Tauber antegrade sclerotherapy can be considered an established procedure for the treatment of varicocele in paediatric age. Herein, we share our experience with this technique and its tricks. The choice of the spermatic vein should be very accurate during the Tauber procedure, and a correct intraoperative phlebography is mandatory to avoid complications.
Crystal structure and Biological evolution of 5-bromothiophene based 3,4-dihydropyrimidin-2-(1H)-thi(ones)
Authors: Ramkumar Venkatachalam , Gardas Ramesh , Banerjee Sourav , Patel Hitendra , Sharma Mayank , Vala Ruturajsinh , Rajani Dhanji 
Published: July 2021

Abstract
Herein, Solvent-free synthesis of 5-bromothiophene based 3,4-dihydropyrimidin-2-(1H)-thi(ones) was explored. The main advantages of this study are that pure product comes in hand with easy workup and without using any separation technique, a good amount of yield, and formation of product in crude form. We developed a single crystal of 4c, and we analysed crystal structure by Hirshfeld surfaces analysis. All the synthesised compounds were screened for antifungal and antibacterial assay. Among all synthesised compounds, 4a, 4b and 4j have potent antibacterial activity. While 4f, 4g, 4h and 4i show good antifungal activity among all synthesised compounds. We also carried out ADMET prediction for the 4(a-j), and calculated data show that all the compounds have prominent drug-like nature.