bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA STK4 antisense RNA 1(STK4-AS1) promoted osteosarcoma by inhibiting p53 expression
Authors: Yao Weitao , Wang Chuchu 
Published: October 2021

Abstract
Background: LncRNA STK4 antisense RNA 1(STK4-AS1) has been identified as a potential biomarker associated with multiple cancers. We proposed that STK4-AS1 plays a role in the proliferation of osteosarcoma by regulating the cell cycle. Methods: We compared the expression of STK4-AS1, p53, and p21 in osteosarcoma vs normal samples in clinical tissues and cell lines. We determined the effect of overexpression and knockdown of STK4-AS1 in p53 expressing osteosarcoma cells U2OS, p53 muted osteosarcoma cells MG63, and osteoblast cells hFOB on p53 and p21 expression and the cell viability. For U2OS and MG63, the cell cycle was analyzed and the expression of cyclin proteins was determined. We overexpressed p53 or p21 in STK4-AS1 overexpressed cells to explore the association of STK4-AS1 and p53 in U2OS. Results: The STK4-AS1 expression was higher and p53 and p21 expression were lower in osteosarcoma tissue and cells than in their non-cancer counterparts. The expression of STK4-AS1 was negatively correlated with the expression of p53 or p21. Knockdown of STK4-AS1 in U2OS decreased the cell viability, increased cells in the G0/G1 phase, decreased cells in the S and G2/M phase, decreased expression of cyclin A and B, increased p53 and p21, and had no effect on cyclin D and cyclin E, while overexpression of STK4-AS1 did the opposes. Overexpression of p53 or p21 recovered some changes caused by STK4-AS1 overexpression in U2OS. MG63 expressed no p53 and the expression of p21, cyclin A, and cyclin B, cell viability, and cell cycle were not affected by altered STK4-AS1 levels. In hFOB cells, the expression of p53 and p21 was decreased and the cell viability was increased when STK4-AS1 was overexpressed, but they were not affected when STK4-AS1 was knocked down. Conclusion: LncRNA STK4-AS1 promoted the cell cycle of osteosarcoma cells by inhibiting p53 expression.
Paramedic Norwegian Acute Stroke Prehospital Project (ParaNASPP) study protocol: a stepped wedge randomised trial of stroke screening using the National Institutes of Health Stroke Scale in the ambulance
Authors: Guterud Mona Marie , Bugge Helge Fagerheim , Bache Kristi CG , Braarud Anne-Cathrine , Eriksen Erik , Fremstad Kjell Otto , Ihle-Hansen Hege , Ingebretsen Svein Håkon , Kramer-Johansen Jo , Larsen Karianne , Røislien Jo , Thorsen Kjetil , Toft Mathias , Sandset Else Charlotte , Hov Maren Ranhoff 
Published: October 2021

Abstract
BackgroundLess than 50% of stroke patients in Norway reach hospital within four hours of symptom onset. Early prehospital identification of stroke and triage to right level of care may result in more patients receiving acute treatment. Quality of communication between paramedics and the stroke centre directly affects prehospital on-scene time, emphasising this as a key factor to reduce prehospital delay. Prehospital stroke scales are developed for quick and easy identification of stroke, but have poor sensitivity and specificity compared to an in-hospital assessment with the National Institutes of Health Stroke Scale (NIHSS). The aim of Paramedic-Norwegian Acute Stroke Prehospital Project (ParaNASPP) is to assess whether a structured learning program, prehospital NIHSS and a mobile application facilitating communication with the stroke physician may improve triage of acute stroke patients. MethodsA stepped wedge cluster randomised controlled intervention design will be used in this trial in Oslo, Norway. Paramedics at five ambulance stations will enrol adult patients with suspected stroke within 24 hours of symptom onset. All paramedics will begin in a control phase with standard procedures. Through an e-learning program and practical training, a random and sequential switch to the intervention phase takes place. A mobile application for NIHSS scoring, including vital patient information for treatment decisions, transferring data from paramedics to the on-call stroke physician at the Stroke Unit at Oslo University Hospital, will be provided for the intervention.The primary outcome measure is positive predictive value (PPV) for prehospital identification of patients with acute stroke defined as the proportion of patients accepted for stroke evaluation and discharged with a final stroke diagnosis. 1300 patients provide a 50% surplus to the 808 patients needed for 80% power to detect a 10% increase in PPV.DiscussionStructured and digital communication using a common scale like NIHSS may result in increased probability for better identification of stroke patients and less stroke mimics delivered to a stroke team for acute diagnostics and treatment in our population. Trial registration: NCT04137874, posted October 24, 2019. https://clinicaltrials.gov/ct2/show/NCT04137874
Acute effect of benchmarck crosstrainig associated with physiological parameters and mental skills according to performance
Authors: de Brito Michele Andrade , Fernandes José Raimundo , Costa Keveenrick Ferreira , Esteves Natã Sant’anna , de Carvalho Pedro Henrique Berbert , Miarka Bianca 
Published: October 2021

Abstract
Present study aimed to evaluate the acute effect of cross training benchmarck on the physiological parameters associated with mental abilities according to performance. Thirty-two practitioners were divided according to their performance, Elite Group (n = 07) Advanced Group (n = 10) and Beginner group (n = 15). This research compares groups and pre-WOD and post-WOD. Result showed significance concerning the performance in WOD Fran (E: 177.1±29.8s <A: 314.3±46.8s and I: 538.2±102.1s), total robustness (A: 50.20±5.24 ˃ E: 43.86±7.47 and I: 43.47±5.81) and control (A: 22.20±3.36 ˃ E: 20.14±3.93 and 19.07±3.65), lactate (E: 13.1±1.8mmol <A: 15.1±3.2mmol and I: 16.1±3.7mmol), heart rate (E : 188.0±6.6bpm ˃ A: 174.1±16.1bpm and I: 185.1±8.9bpm), systolic blood pressure (E: 149.7±11.5mmHg <A: 151.0±9.2mmHg and I: 152.5±8.1mmHg), and diastolic blood pressure (E: 73.4±6.2mmHg ˃ A: 72.8±9.6mmHg and I: 69.3±7.1mmHg). Significant correlations in the Elite group in lactate and appearance and systolic blood pressure and choice. Advanced Group significant correlations in WOD time and extroversion, lactate and confidence and heart rate and reading. Beginner Group significant correlations in WOD time and Achievement, lactate and constancy and lactate and flexibility. Present study concluded that about mental abilities, Advanced level athletes showed greater total strength than athletes from Elite and Beginner groups.
Randomised Controlled Trial To Investigate The Effectiveness of The Self-Management After Radiotherapy (SMaRT) Intervention To Ameliorate Lower Urinary Tract Symptoms in Men Treated for Prostate Cancer
Authors: Faithfull Sara , Cockle-Hearne Jane , Lemanska Agnieszka , Otter Sophie , Skene Simon 
Published: October 2021

Abstract
Purpose

To evaluate the effectiveness of the self-management after radiotherapy (SMaRT) intervention to improve urinary symptoms in men with prostate cancer.
Methods

The randomised controlled trial (RCT) recruited men from one radiotherapy centre in the UK after curative radiotherapy or brachytherapy and moderate urinary symptoms defined as the International Prostate Symptom Score (IPSS) ≥ 8. 63 men were randomised either to SMaRT, a 10-week self-management intervention including group support, education, pelvic floor muscle exercises, or care-as-usual. The primary outcome was the IPSS at 6 months. Secondary outcomes were IPSS at 3 months, and International Continence Society Male Short Form (ICS), European Organisation for Research and Treatment of Cancer Quality of Life prostate scale (EORTC QLQ-PR25), EORTC QLQ-30 and Self-Efficacy for Symptom Control Inventory (SESCI) at 3 and 6 months. Analysis of covariance (ANCOVA) was used to analyse the effect of the intervention.
Results

SMaRT did not improve urinary symptoms as measured by IPSS at 6 months. The adjusted difference was − 2.5 [95%CI -5.0 to 0.0], p = 0.054. Significant differences were detected at 3 months in ICS voiding symptoms (-1.1 [-2.0 to -0.2], p = 0.017), ICS urinary incontinence (-1.0 [-1.8 to -0.1], p = 0.029) and SESCI managing symptoms domain (13.5 [2.5 to 24.4], p = 0.017). No differences were observed at 6 months.
Conclusions

SMaRT provided short-term benefit in urinary voiding and continence, and helped men manage symptoms but was not effective long-term. Face-to-face and supervised approaches may provide more benefit.
Patient Empowerment Among People Attending Cancer Follow-Up – Results from a Nationwide Questionnaire Survey
Authors: Eskildsen Nanna Bjerg , Ross Lone , Jørgensen Clara Rübner , Pedersen Susanne S , Thomsen Thora Grothe , Groenvold Mogens , Johnsen Anna Thit 
Published: October 2021

Abstract
PurposeTo investigate levels of empowerment, possibilities for empowerment and perceived importance of empowerment among Danes in cancer follow-up. MethodsFrom nation-wide registers a randomly selected group of people diagnosed with one of ten different cancer diagnoses between one and five years ago were invited to complete the newly developed ‘Cancer Patient Empowerment Questionnaire’ (CPEQ). Respondents who reported that they were in a follow-up program were included in the analysis. Frequencies of answers to the items in the questionnaire were calculated. ResultsIn total, 1,418 people with a diagnosis of cancer returned the questionnaire (response rate 54%). Of these, 1,042 reported being in follow-up and were included in the analysis. Most respondents experienced that there was a plan for their follow-up (83%) and felt at ease with both the frequency (80%) and content of the follow-up (78%). However, many also reported lack of possibilities for and/or low levels of empowerment. They were not aware of any decisions being made during their follow-up, had not received information about relevant patient associations, or had any conversations with healthcare professionals regarding their needs and wishes for their follow-up. Furthermore, almost 20% lacked information on and confidence in managing treatment-related side-effects, late complications and alarm symptoms. ConclusionMany respondents reported lack of possibilities for and/or low levels of empowerment in their cancer follow-up. This may have consequences for their quality of life and capabilities and opportunities for managing their own care.
The Effect of Operating Parameters of Alkali Catalyzed Transesterification of Sunflower Oil With Methanol in the Presence of the Cosolvent Assisted by Hydrodynamic Cavitation on the Degree of Triglyceride Conversion
Authors: Nikolić Ivan , Jovanović Jelena , Koturević Biljana , Adnadjević Borivoj 
Published: October 2021

Abstract
Method of independent variation of the value of one operating parameter has been used to investigate the effect of operating parameters on alkali-catalyzed transesterification of sunflower oil with methanol in presence of tetrahydrofuran (THF) as cosolvent, assisted by hydrodynamic cavitation (ACTC) on the value of the degree of triglyceride conversion (DTC). ACTC was performed by a venturi-type hydrodynamic cavitation reactor (VCR) of our construction. To determine the effect of ACTC on DTC following operating parameters were varied: reaction mixture inlet pressure (p1) from 202.3 kPa to 1013.2 kPa; methanol to oil molar ratio (M1) from M1=3 to M1=12; concentration of catalyst (Cc) from 0.3wt% to 1.5wt%; methanol to THF molar ratio (M2) from M2=1.25 to M2=2.0; temperature (T) from 20°C to 55°C, number of passes through the VCR (n) from n=1 to n=10. It was found, based on the obtained results, that: a) the values of DTC increase with the increase in p1, M1, Cc, and n, b) the values of the DTC decrease with the increase in T and c) maximum values of the DTC are obtained at Cc=1.0~1.1wt% and M2=1.5.
PEGylated Nanoemulsions Containing 1,2-Distearoyl-sn-Glycero-3-Phosphoglycerol Induced Weakened Accelerated Blood Clearance Phenomenon
Authors: Li Zhe , Gao Xin , Yan Xinyang , Deng Yuihui , Ma Haiying 
Published: October 2021

Abstract
Injections of polyethylene glycol (PEG)-modified nanomedicines can lead to an accelerated clearance of the next dose of PEGylated nanomedicines, which is referred to as the accelerated blood clearance (ABC) phenomenon. It has been reported that anti-PEG IgM plays an important role in the induction of the ABC phenomenon, identifying the interface between the main chain of PEG and the hydrophobic segment of the repeated injections of the PEGylated nanocarriers, resulting in increased liver uptake and loss of long cycle characteristics. In this study, we demonstrated that the 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) in PEGylated nanoemulsions (PEs) may mask this interface between the main chain of PEG and the hydrophobic segment, inhibiting the recognition and binding of anti-PEG IgM to PEs, and evidently weakening the ABC phenomenon of PEs. This will provide a novel strategy to improve the curative effect of PEGylated nanocarriers.
Intramolecular Orthogonal Reporters for Dissecting Oxidative Stress and Response
Authors: Pan Yecan , Weng Rui , Zhang Linghao , Qiu Jing , Wang Xinlu , Liao Guangqin , Qin Zhaohui , Liu Rui , Dai Xiaochuan , Qian Yongzhong , Su Xin 
Published: October 2021

Abstract
Molecular response under oxidative stress is a well-known reflection of the development of various diseases. Fluorescent probes for reactive oxygen species (ROS) and for their related molecules integrated into single nanostructures could simultaneously map the localization and abundance of multiple targets with spatiotemporal accuracy in live cells. However, it is extremely challenging because proximity-induced fluorophore interaction leads to low sensitivity and poor specificity. Herein, we engineered intramolecular Orthogonal Reporters (iOR) based on DNA nanostructure scaffolds. Fluorescence reporters in iOR were precisely located with appropriate distance achieved by MD simulation. iOR functionalized with nuclear localization signal peptide is capable of imaging ROS and its related gene repair enzyme (APE1) in nucleus and cytoplasm with spatiotemporal accuracy. iOR discloses the strong positive relation of ROS and APE1 as well as their synergistic regulation. In vivo fluorescence imaging with iOR enables direct observation of ROS and APE1 of the tumor sites of mice. iOR represents a new concept of molecule probes for simultaneous detection of multiple related targets in living cells. We anticipate this simulation-guided probe to find broad applications in cellular biology and nanotechnology.
‘Subacute’ Onset of Guillain Barré Syndrome Post mRNA-1273 Vaccination: A Case Report
Authors: Nagalli Shivaraj , Kikkeri Nidhi Shankar 
Published: October 2021

Abstract
Ever since the start of the pandemic, SARS-CoV-2 has taken the lives of millions of people around the globe. Several COVID-19 vaccines have been developed with rapidity to prevent acquiring COVID-19 infection, hospitalizations and deaths. The routine side effects of these vaccines are commonly known and non-severe. Few serious side-effects such as thrombosis with thrombocytopenia syndrome (TTS) and Guillain Barré Syndrome (GBS) are increasingly reported particularly after inoculation with ChAdOx1 nCoV-19 (Oxford/AstraZeneca) and Ad26.COV 2.S (Johnson & Johnson’s Janssen). Rare cases of GBS after BNT162b2 (Pfizer-BioNTech), an mRNA vaccine are also reported. However, the true association of these cases to COVID-19 continues to be unclear and the safety of these vaccines continues to be great in preventing deaths from COVID-19 infection. We report a case of middle-aged female who had a gradual onset of lower extremity weakness with a nadir of symptoms reached 10 and 12 weeks after the onset. This protracted course (sub-acute) is atypical for a ‘classical’ GBS. The presence of an antecedent event, autonomic symptoms such as hypotension and the need for ventilator support favored the diagnosis of GBS than chronic inflammatory demyelinating polyneuropathy (CIDP). This is the first known case to be reported of sub-acute onset of Guillain Barré Syndrome after receiving the mRNA-1273 vaccine.
Multi-tissue proteomics identifies molecular signatures for sporadic and genetically defined Alzheimer disease cases
Authors: Cruchaga Carlos , Sung Yun Ju , Yang Chengran , Wang Fengxian , Suhy Adam , Norton Joanne , Novotny Brenna , Etelleb Abdallah , Fagan Anne , Bateman Randall , Perrin Richard , Morris John , Farlow Martin , Chhatwal Jasmeer , Chui Helena , Rhinn Herve , Karch Celeste , Schindler Suzanne , Harari Oscar 
Published: October 2021

Abstract
Alzheimer disease (AD) is a heterogeneous disease with many genes are associated with AD risk. Most proteomic studies, while instrumental in identifying AD pathways and genes, focus on single tissues and sporadic AD cases. Multi-tissue proteomic signatures for sporadic and genetically defined AD (e.g., pathogenic variant carriers in APP and PSEN1/2 and risk variant carriers in TREM2) will illuminate the biology of this heterogeneous disease.1,2 Here, we present one of the largest multi-tissue proteomic profiles, accessible through our web portal, based on 1,305 proteins in brain (n=360), cerebrospinal fluid (CSF; n=717), and plasma (n=490) from the Knight Alzheimer Disease Research Center (Knight ADRC) and Dominantly Inherited Alzheimer Network (DIAN) cohorts.3-5 We identified proteomic signatures in brain, CSF, and plasma for sporadic AD status and replicated these findings in multiple, independent datasets. The area under the curve (AUC) for CSF proteins was 0.89 in discovery and 0.90 in the replication dataset, which was significantly higher than the AUC for CSF p-tau181/Aβ42 (AUC = 0.81; P = 2.4×10-6). We also identified a specific proteomic signature for TREM2 variant carriers that differentiated TREM2 variant carriers from sporadic AD cases and controls with high sensitivity and specificity (AUC = 0.81 - 1). In addition, the proteins that showed differential levels in sporadic AD were also altered in autosomal dominant AD, but with greater effect size (1.4 times, P = 3.8×10-5), and proteins associated with autosomal dominant AD, in brain tissue also replicated on CSF (p=1.36×10-9). Enrichment analyses highlighted several pathways including AD (calcineurin, APOE, GRN), Parkinson disease (α-synuclein, LRRK2), and innate immune response (SHC1, MAPK3, SPP1) for the sporadic AD or TREM2 variant carriers. Our findings show the power of multi-tissue proteomics’ contribution to the understanding of AD biology and to the creation of tissue-specific prediction models for individuals with specific genetic profiles, ultimately supporting its utility in creating individualized disease risk evaluation and treatment.
Patients Perception of Pharmacovigilance system: A Pre-Diagnostic and Post-Interventional Cross-Sectional Survey
Authors: Rehman Atta Ur , Khalid Samina Naeem , Hani Ume 
Published: October 2021

Abstract
Background & Objective The risk of adverse reactions necessitated the pharmacovigilance system for patient safety. Literature search documented better health literacy of patients through intervention. This investigation aims to assess the perception and the effect of an intervention on patients regarding adverse reactions caused by drugs.MethodsA pre-diagnostic and post-interventional cross-sectional investigation was designed in hospitals of Islamabad. The proportion of patients was selected based on a stratified probability technique. A prevalidated tool was used to collect the response twice through health promotion brochure with counseling was applied as an intervention.Results The findings of the study disclosed that health intervention improved health literacy among patients. Knowledge of pharmacovigilance was known by 97.9% of the patients in the interventional survey compared to a diagnostic survey in which the majority of the sampled population was not aware. The outcome of intervention on paired variables of knowledge, communication, and practice showed significant differences at the 0.05 level by using t-test statistics.ConclusionThe finding of the investigation provided evidence that patient awareness significantly improved by the health promotion model. Patient participation in the reporting of adverse reactions of drugs will complement the hospital staff reporting. These reports will construct an authentic cross-checked database for rational drug safety practices in Pakistan.
English Classroom Immersive Teaching Based on Digital Twin Technology
Authors: da Zhenjing , gu shan , Huang Yanan 
Published: October 2021

Abstract
In order to improve the effect of English classroom teaching, this paper builds an immersive teaching model of English classroom based on digital twin technology. Moreover, in order to reduce the cost of the system, simplify the system structure, and enable learners to observe the stereoscopic display image with the naked eye, this paper builds an immersive hemispherical projection teaching system, designs the overall structure of the system and various components of the hardware, and studies the projection image predistortion algorithm. In addition, this paper uses the immersive experience between virtual reality technology and immersive teaching as a connection point to design an immersive teaching based on VR and apply it to teaching practice to test the effect of immersive teaching, and perfect the immersive teaching design model based on VR. Finally, this paper designs an experiment to verify and analyze the performance of the system. From the experimental results, it can be seen that the English classroom immersive teaching model based on digital twin technology constructed in this paper is effective.
Expanding the Reach of RNAi Therapeutics with Next Generation Lipophilic siRNA Conjugates
Authors: Brown Kirk , Nair Jayaprakash , Janas Maja , Anglero-Rodriguez Yesseinia , Peng Haiyan , Theile Christopher , Castellanos-Rizaldos Elena , Brown Christopher , Foster Donald , Kurz Jeffrey , Allen Jeffrey , Maganti Raj , Li Jing , Matsuda Shigeo , Chickering Tyler , Jung Michelle , Wassarman Kelly , Rollins Jeff , Woods Lauren Woods Lauren , Kelin Alex , Gunther Dale , Mobley Melissa , Petrulis John , McDougall Robin , Racie Tim , Bombardier Jessica , Cha Diana , Agarwal Saket , Johnson Lei , Jiang Yongfeng , Lentini Scott , Gilbert Jason , Nguyen Tuyen , Chigas Samantha , Blanc Sarah Le Blanc Sarah Le , Poreci Urjana , Kasper Anne , Rogers Arlin , Chong Saeho , Davis Wendell , Sutherland Jessica , Castoreno Adam , Milstein Stuart , Schlegel Mark , Zlatev Ivan , Charisse Klaus , Keating Mark , Manoharan Muthiah , Fitzgerald Kevin , Wu Jing-Tao , Maier Martin , Jadhav Vasant 
Published: October 2021

Abstract
RNA interference (RNAi) therapeutics are a new class of medicines that can address unmet medical needs by silencing disease-causing gene transcripts. While delivery of short interfering RNAs (siRNAs) to hepatocytes has yielded multiple drug approvals, novel delivery solutions are needed to expand the reach of RNAi therapeutics. Here we report that conjugation of 2'-O-hexadecyl (C16) to siRNAs enables efficient silencing in the central nervous system (CNS), eye, and lung of multiple nonclinical species with broad cell type specificity. Intrathecally delivered C16-siRNAs are active across CNS regions and cell types, with sustained silencing for at least three months, which is an especially important outcome considering the challenging dosing route. Similarly, intravitreal and intranasal administration of C16-siRNAs resulted in potent and sustained knockdown in the eye and lung, respectively. Efficient delivery facilitated through C16 conjugation to optimized siRNA designs has enabled candidate selection for investigational human clinical trials assessing therapeutic silencing beyond the liver with infrequent (e.g. bi-annual) dosing.