bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploitaton of the Functional Properties of Leuconostoc mesenteroides and Lactobacillus plantarum as Probiotic using Soyabeans Flour as Vehicle
Authors: Musliu Abdulkadir , Yusuf Muhammed , Adebisi Sulaimon 
Published: July 2021

Lactic acid bacteria produce a variety of antimicrobial compounds such as acetic acid, hydrogen peroxide, diacetyl on them as a natural competitive means to overcome other microorganism sharing the same niche. Seven different strains of Leuconostoc mesenteroides and Lactobacillus plantarum were screened for their ability to produce enzymes and metabolites. They were also screened for their ability to withstand some physiological stress like acid tolerance, temperature, salt concentration and antibacterial activity. Leuconostoc mesenteroides S3 and Lactobacillus plantarum Yh1produced values significantly different to other five isolates and they were selected. These functional properties were exploited in the fermentation of soyabean in order to obtain a probiotic vehicle. The selected isolates were used as starter both singly and in consortium. This research work also finds out the suitability of soya beans flour as a vehicle for probiotic microorganisms (Lactobacillus plantarum and Leuconostoc mesenteroides). The cleaned, dried and roasted soya beans were grinded to flour. The soya beans flour was fermented with Lactobacillus plantarum and Leuconostoc mesenteroides and there was mass increase in lactic acid counts in the fermented soya beans flour store at room temperature. All lactic acid fermented soya beans flour recorded increase at room temperature but slight reduction in number at refrigerator temperature from day 0 to day 14 of storage. Lactobacillus plantarum fermented soya beans flour (LPFSB) recorded increase from 5.9x105 (day 0) to 10.60x105cfu/g (day 14), while the L. plantarum stored at refrigeration temperature had 5.9x105cfu/g on (day 0) to 5.5x105cfu/g on day 14. The L. plantarum and consortium of both starters used were observed to have considerable increase in cell growth after storage, therefore this satisfies the criteria for good probiotic bacteria. Leuconostoc mesenteroides fermented soya beans slightly reduced in cell number at pH of 2 but others survived well at acidic pH and 10% bile. The cell number reduced from 4.8x106 (initial) to 4.2x106 (final). The pH of the intestine ranges from 2.5 to 3.5 and the starter culture used survive this pH and the bile product which shows greatly that they can serve as probiotic in the treatment of infection in gastrointestinal tract .
Species‐specific demographic and behavioral responses to food availability during migratory stopover
Authors: Tucker Anna M. , McGowan Conor P. , Lyons James E. , DeRose‐Wilson Audrey , Clark Nigel A. 
Published: July 2021

AbstractUnderstanding the effects of migratory stopover site conditions on both demographic rates and migratory behaviors is critical for interpreting changes in passage population sizes at stopover sites and predicting responses to future changes and conservation actions. We used a Bayesian formulation of the open robust design model to analyze mark‐resight observations of three migratory shorebird species using Delaware Bay, USA during spring stopover from 2005 to 2018. We tested for an effect of stopover food availability and weather conditions on survival probability and the probability of returning to this site in the next year and found species differences in these relationships. After years with greater food availability, red knot Calidris canutus rufa had higher survival probability but ruddy turnstone Arenaria interpres were more likely to return to the site. Estimates of within‐year probabilities of arrival and persistence at the stopover site showed relatively consistent migration schedules for ruddy turnstone, but more interannual variation for red knot and sanderling Calidris alba. Shorebird use of this site typically peaked during May 26–28, but the estimated proportion of the population present during this period varied dramatically among years for red knot (range: 0.07–0.59) but less so for ruddy turnstone and sanderling. This demonstrates that both the proportion of the flyway population using this stopover site and the proportion present during a given sampling period vary among years, and both should be considered in analyzing and interpreting monitoring data. Stopover conditions can influence both migratory behavior and demographics, underscoring the importance of flyway‐wide monitoring.
Use of Complementary and Alternative Therapy for Knee Osteoarthritis: Race and Gender Variations
Authors: Vina Ernest R. , Youk Ada O. , Quinones Cristian , Kwoh C. Kent , Ibrahim Said A. , Hausmann Leslie R.M. 
Published: July 2021

ObjectiveTo evaluate race and gender variations in complementary and alternative medicine (CAM) use for knee osteoarthritis (OA) (unadjusted and adjusted for demographic and clinical factors).MethodsA secondary analysis of cross‐sectional data was conducted. The sample included Veterans Affairs patients 50 years of age or older with symptomatic knee OA. Current use of various CAM therapies was assessed at baseline. Logistic regression models were used to compare race and gender differences in the use of specific CAMs.ResultsThe sample included 517 participants (52.2% identified as African American [AA]; 27.1% identified as female). After adjusting for demographic and clinical factors, AA participants, compared with white participants, were less likely to use joint supplements (odds ratio [OR]: 0.53; 95% confidence interval [CI], 0.31‐0.90]); yoga, tai chi, or pilates (OR: 0.39; 95% CI: 0.19‐0.77); and chiropractic care (OR: 0.51; 95% CI: 0.26‐1.00). However, they were more likely to participate in spiritual activities (OR: 2.02; 95% CI: 1.39‐2.94). Women, compared with men, were more likely to use herbs (OR: 2.42; 95% CI: 1.41‐4.14); yoga, tai chi, or pilates (OR: 2.09; 95% CI: 1.04‐4.19); acupuncture, acupressure, or massage (OR: 2.45; 95% CI: 1.28‐4.67); and spiritual activities (OR: 1.68; 95% CI: 1.09‐2.60). The interactive effects of race and gender were significant in the use of herbs (P = 0.008); yoga, tai chi, or pilates (P = 0.011); acupuncture, acupressure or massage (P = 0.038); and spiritual activities (P < 0.001).ConclusionThere are race and gender differences in the use of various CAMs for OA. As benefits and limitations of CAM therapies vary, clinicians must be aware of these differences.
Highly Reliable Charge Trap‐Type Organic Non‐Volatile Memory Device Using Advanced Band‐Engineered Organic‐Inorganic Hybrid Dielectric Stacks
Authors: Kim Min Ju , Lee Changhyeon , Shin Eui Joong , Lee Tae In , Kim Seongho , Jeong Jaejoong , Choi Junhwan , Hwang Wan Sik , Im Sung Gap , Cho Byung Jin 
Published: July 2021

AbstractWith the recent interest in data storage in flexible electronics, highly reliable charge trap‐type organic‐based non‐volatile memory (CT‐ONVM) has attracted much attention. CT‐ONVM should have a wide memory window, good endurance, and long‐term retention characteristics, as well as mechanical flexibility. This paper proposed CT‐ONVM devices consisting of band‐engineered organic–inorganic hybrid films synthesized via an initiated chemical vapor deposition process. The synthesized poly(1,3,5‐trimethyl‐1,3,5,‐trivinyl cyclotrisiloxane) and Al hybrid films are used as a tunneling dielectric layer and a blocking dielectric layer, respectively. For the charge trapping layer, different Hf, Zr, and Ti hybrids are examined, and their memory performances are systematically compared. The best combination of hybrid dielectric stacks showed a wide memory window of 6.77 V, good endurance of up to 104 cycles, and charge retention of up to 71% after 108 s even under the 2% strained condition. The CT‐ONVM device using the hybrid dielectric stacks outperforms other organic‐based charge trap memory devices and is even comparable in performance to conventional inorganic‐based poly‐silicon/oxide/nitride/oxide/silicon structures devices. The CT‐ONVM using hybrid dielectrics can overcome the inherent low reliability and process complexity limitations of organic electronics and expedite the realization of wearable organic electronics.
Universal Peptide Hydrogel for Scalable Physiological Formation and Bioprinting of 3D Spheroids from Human Induced Pluripotent Stem Cells
Authors: Li Quan , Qi Guangyan , Liu Xuming , Bai Jianfa , Zhao Jikai , Tang Guosheng , Zhang Yu Shrike , Chen‐Tsai Ruby , Zhang Meng , Wang Donghai , Zhang Yuanyuan , Atala Anthony , He Jia‐Qiang , Sun Xiuzhi Susan 
Published: July 2021

AbstractHuman induced pluripotent stem cells (hiPSCs) are used for drug discoveries, disease modeling and show great potential for human organ regeneration. 3D culture methods have been demonstrated to be an advanced approach compared to the traditional monolayer (2D) method. Here, a self‐healing universal peptide hydrogel is reported for manufacturing physiologically formed hiPSC spheroids. With 100 000 hiPSCs encapsulated in 500 µL hydrogel, ≈50 000 spheroids mL−1 (diameter 20–50 µm) are generated in 5 d. The spheroids in the universal peptide hydrogel are viable (85–96%) and show superior pluripotency and differentiation potential based on multiple biomarkers. Cell performance is influenced by the degradability of the hydrogel but not by gel strength. Without postprinting crosslinking aided by UV or visible lights or chemicals, various patterns are easily extruded from a simple star to a kidney‐like organ shape using the universal peptide hydrogel bioink showing acceptable printability. A 20.0 × 20.0 × 0.75 mm3 sheet is finally printed with the universal peptide hydrogel bioink encapsulating hiPSCs and cultured for multiple days, and the hiPSC spheroids are physiologically formed and well maintained.
Graphene‐Based Heterostructure Composite Sensing Materials for Detection of Nitrogen‐Containing Harmful Gases
Authors: Feng Qiuxia , Huang Baoyu , Li Xiaogan 
Published: July 2021

AbstractGraphene‐based heterostructure composite is a new type of advanced sensing material that includes composites of graphene with noble metals/metal oxides/metal sulfides/polymers and organic ligands. Exerting the synergistic effect of graphene and noble metals/metal oxides/metal sulfides/polymers and organic ligands is a new way to design advanced gas sensors for nitrogen‐containing gas species including NH3 and NO2 to solve the problems such as poor stability, high working temperature, poor recovery, and poor selectivity. Different fabrication methods of graphene‐based heterostructure composite are extensively studied, enabling massive progress in developing chemiresistive‐type sensors for detecting the nitrogen‐containing gas species. With the components of noble metals/metal oxides/metal sulfides/polymers and organic ligands which are composited with graphene, each material has its attractive and unique electrical properties. Consequently, the corresponding composite formed with graphene has different sensing characteristics. Furthermore, working ambient gas and response type can affect gas‐sensitive characteristic parameters of graphene‐based heterostructure composite sensing materials. Moreover, it requires particular attention in studying gas sensing mechanism of graphene‐based heterostructure composite sensing materials for nitrogen‐containing gas species. This review focuses on related scientific issues such as material synthesis methods, sensing performance, and gas sensing mechanism to discuss the technical challenges and several perspectives.
Scaling Metal‐Elastomer Composites toward Stretchable Multi‐Helical Conductive Paths for Robust Responsive Wearable Health Devices
Authors: Zhao Yue , Tan Yu Jun , Yang Weidong , Ling Shaohua , Yang Zijie , Teo Ju Teng , See Hian Hian , Lee David Kwok Hung , Lu Dingjie , Li Shihao , Zeng Xianting , Liu Zhuangjian , Tee Benjamin C.K. 
Published: July 2021

AbstractStretchable electronics have advanced rapidly and many applications require high repeatability and robustness under various mechanical deformations. It has been described here that how a highly stretchable and reliable conductor composite made from helical copper wires and a soft elastomer, named eHelix, can provide mechanically robust and strain‐insensitive electronic conductivity for wearable devices. The reversibility of the mechanical behavior of the metal‐elastomer system has been studied using finite element modeling methods. Optimal design parameters of such helical metal‐elastomer structures are found. The scaling of multiple copper wires into such helical shapes to form a Multi‐eHelix system is further shown. With the same elastomer volume, Multi‐eHelix has more conductive paths and a higher current density than the single‐eHelix. Integrations of these eHelix stretchable conductors with fabrics showed wearable displays that can survive machine‐washes and hundreds of mechanical loading cycles. The integration of the eHelix developed by us with a wearable optical heart rate sensor enabled a wearable health monitoring system that can display measured heart rates on clothing. Furthermore, Multi‐eHelix conductors are used to connect flexible printed circuit boards and piezoresistive sensors on a tactile sensing glove for the emerging sensorized prosthetics.
Iridium Oxide Nanoparticle–Protein Corona Neural Interfaces with Enhanced Electroactivity and Bioactivity Enable Electrically Manipulatable Physical and Chemical Neuronal Activation
Authors: Chan Fu‐Erh , Syu Huei‐Min , Wang Te‐Yi , Tang Zheng‐Ting , Huang Chih‐Ning , Lee Jyh‐Fu , Burnouf Thierry , Hu Shang‐Hsiu , Chen Po‐Chun , Huang Wei‐Chen 
Published: July 2021

AbstractIridium oxide (IrOx) is a promising implantable electrode material owing to its remarkable neural stimulation capacity. However, presently, IrOx electrodes lack biocompatibility and bioactive interactions with nerve tissues. Application of polymeric surface coatings results in a weak physical adhesion at the organic/inorganic interface, which limits their wide‐scale application. Herein, a smart iridium oxide‐plasma protein (IrOx‐PP) electrode with enhanced electroactivity, electrochemical stability, cytocompatibility, and bioactivity that can provide controllable topographical, electrical, and chemical stimuli to enhance neuronal activity is proposed. In the inorganic/organic nanoparticle (NP)‐protein corona structures, the soft NP‐corona led to repeated burst‐to‐zero‐to‐burst PP release, while the hard NP‐corona with an ordered atomic structure enhanced the electrochemical stability and bioactivity. The incorporated PP resulted in a higher current storage capacity, lower impedance, better cell growth, and significant neurite outgrowth compared with those obtained with pristine IrOx. The application of electrical stimulation to IrOx‐PP enabled simultaneous neuromodulation, on‐demand PP release, and cell uptake, with a 2‐fold higher cell density and significant neurite outgrowth on IrOx‐PP than on pristine IrOx. This bioactive inorganic‐organic hybrid electrode with the combined features of physical properties and improved neuromodulation is expected to be a revolutionary platform for efficient and biocompatible neural implantation.
Fluorescent Silicon Carbide Nanoparticles
Authors: de Vries Mitchell O. , Sato Shin‐ichiro , Ohshima Takeshi , Gibson Brant C. , Bluet Jean‐Marie , Castelletto Stefania , Johnson Brett C. , Reineck Philipp 
Published: July 2021

AbstractSilicon carbide (SiC) is an indirect wide band gap semiconductor that is utilized in many industrial applications due to its extreme physical properties. SiC nanoparticles (NPs) exhibit a versatile surface chemistry, fluoresce from the ultraviolet to the near‐infrared spectral ranges, and their sizes can be tuned from one to hundreds of nanometers. Yet, fluorescent SiC NPs have received far less attention by the scientific community. This review summarizes the state‐of‐the‐art in fluorescent SiC NPs. Nanoparticle fabrication methods, characterization techniques, nanoparticle surface chemistry, and SiC NPs fluorescence properties are assessed in detail. Atomic defects and impurities in the SiC crystal lattice (so‐called color centers), surface‐induced fluorescence, quantum confinement, and band‐edge fluorescence are identified as the main sources of fluorescence in SiC NPs. While many color centers are reported in bulk SiC, only few are identified in SiC NPs and interface‐related defects remain poorly understood, creating enormous potential for scientific discovery. Finally, an overview of demonstrated and emerging potential applications of SiC NPs in the areas of bioimaging and quantum sensing is provided.
Efficient Restraint of Intra‐Cluster Aggregation‐Caused Quenching Effect Lighting Room‐Temperature Photoluminescence
Authors: Chen Ling , Dong Xiao‐Bin , Mo Zong‐Wen , Wang Hai‐Ping , Ye Jia‐Wen , Zhang Kun , Chen Xiao‐Ming 
Published: July 2021

AbstractAt room temperature (RT), the weak and strong cluster‐centered luminescence of silver and copper iodide clusters, respectively, has been widely reported. Though the central metal ions show same d10 electrons and similar coordination mode, the mechanism of their distinct emission properties is still unclear, which restricts further applications. In this work, it is found that different photoluminescence quantum yields (PLQYs) are caused by various collision energy transfers between the metal ions in clusters, like a kind of aggregation‐caused quenching mechanism. Concretely, isostructural coordination polymers (CPs) of Ag(I) and Cu(I) iodide clusters are synthesized. The Ag(I)‐CP shows no luminescence at RT while the PLQY of Cu(I)‐CP boosts to 89%, in which short radius and weak collision between metal ions are found. Moreover, shorter Cu(I)‐Cu(I) and longer Ag(I)‐Ag(I) distances are realized in the similar structure and they display prospective luminescence, which proves the collisional quenching mechanism. Inspired of the above, the heterometallic materials with high sensitivities and wide sensing windows to temperature detection are obtained through tuning the metal content, and they are also fabricated into detecting sequences, which satisfies the wide‐range temperature sensing. This work proposes an efficient strategy to improve RT photoluminescence and broaden temperature sensing windows.
Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases
Authors: Ait Belkacem Ines , Mossadegh‐keller Noushine , Bourgoin Penelope , Arnoux Isabelle , Loosveld Marie , Morange Pierre‐emmanuel , Markarian Thibaut , Michelet Pierre , Busnel Jean Marc , Roulland Sandrine , Galland Franck , Malergue Fabrice 
Published: July 2021

AbstractBlood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the central laboratories is currently needed because cells and biomarkers degrade rapidly. The dried blood spot from a fingerstick allows the preservation of cellular molecules for months but entire cells are never recovered. Here leucocyte elution is optimized from dried blood spots. Flow cytometry and mRNA expression profiling are used to analyze the recovered cells. 50–70% of the leucocytes that are dried on a polyester solid support via elution after shaking the support with buffer are recovered. While red blood cells lyse upon drying, it is found that the majority of leucocytes are preserved. Leucocytes have an altered structure that is improved by adding fixative in the elution buffer. Leucocytes are permeabilized, allowing an easy staining of all cellular compartments. Common immunophenotyping and mRNAs are preserved. The ability of a new biomarker (CD169) to discriminate between patients with and without Severe Acute Respiratory Syndrome induced by Coronavirus 2 (SARS‐CoV‐2) infections is also preserved. Leucocytes from blood can be dried, shipped, and/or stored for at least 1 month, then recovered for a wide variety of analyses, potentially facilitating biomedical applications worldwide.
Food product launch and positioning in China—Do claims of credence attributes matter?
Authors: Yang Wei , Renwick Alan , Tantiwat Waranan , Revoredo‐Giha Cesar , Wu Dafeng 
Published: July 2021

AbstractThis article aims to empirically investigate the role of claims of credence attributes in the launch of food and drink products using a large dataset of new product development by food companies in China. A multinomial regression model is employed to analyze the association between the type of product launch and the positioning claims adopted by firms. The results provide evidence that claims of credence attributes play an important role in the product launch strategy of firms. Food companies tend to choose the most cost‐effective strategies to launch products with credence attributes. Hence, claims that are seen as most important for consumers are more likely to be engendered for the more costly launch approach. For example, the attribute of food safety is highly important in China and its use is related to launching brand new products which generally is the most costly launch strategy. In contrast, other credence attributes, such as “environmentally sustainable,” are linked to relatively simpler and cheaper strategies, such as new packaging. Given most studies on credence attributes have focused on the consumer side, results of this study fill in the gap of understanding how food suppliers respond to consumer demand for food products with credence attributes.
Phylogenomics and biogeography of Cunoniaceae (Oxalidales) with complete generic sampling and taxonomic realignments
Authors: Pillon Yohan , Hopkins Helen C. F. , Maurin Olivier , Epitawalage Niroshini , Bradford Jason , Rogers Zachary S. , Baker William J. , Forest Félix 
Published: July 2021

PremiseCunoniaceae are a family of shrubs and trees with 27 genera and ca. 335 species, mostly confined to tropical and wet temperate zones of the southern hemisphere. There are several known issues regarding generic limits, and the family also displays a number of intriguing long‐range disjunctions.MethodsWe performed a phylogenomic study using the universal Angiosperms353 probe set for targeted sequence capture. We sampled 37 species covering all genera in the Cunoniaceae, and those in the three closely related families of the crown Oxalidales (Brunelliaceae, Cephalotaceae, and Elaeocarpaceae). We also performed analyses for molecular dating and ancestral area reconstruction.ResultsWe recovered the topology (Cunoniaceae, (Cephalotaceae, (Brunelliaceae, Elaeocarpaceae))) and a well‐resolved genus‐level phylogeny of Cunoniaceae with strongly supported clades corresponding to all previously recognized tribes. As previously suspected, the genera Ackama and Weinmannia were recovered as paraphyletic. Australasia was inferred as the likely ancestral area for the family.ConclusionsThe current distribution of Cunoniaceae is best explained by long‐distance dispersal with a few possible cases of Australasian–American vicariance events. Extinctions may have been important in determining the mostly Oceanian distribution of this family while some genera in the tribe Cunonieae and in New Caledonia have undergone recent bursts of diversification. New generic diagnoses, 80 new combinations, and one new name are provided for a recircumscribed Ackama (including Spiraeopsis), a much smaller Weinmannia (mostly New World), and a resurrected Pterophylla to accommodate Old World taxa previously in Weinmannia.