bluuebunny/crossref_metadata_2025_split huggingface dataset:
Embryonal Rhabdomyosarcoma of Prostate in Adult: A Rare Case Report
Authors: Andhika Dimas Panca , Hardjowijoto Sunaryo 
Published: July 2021

Rhabdomyosarcoma is the fifth most common type of soft tissue solid tumor in children and the most common in the last two decades. Rhabdomyosarcoma of the urogenital organ is a rare mesenchymal tumor, covering 22% of all Rhabdomyosarcoma cases. The two most common histologic types are alveolar and embryonal, whereas botryoid and spindle cells are rarely found. We reported a case of embryonal Rhabdomyosarcoma of the prostate. In this study, we improved the understanding of Embryonal Rhabdomyosarcoma of the prostate on 23 years old male who had a history of swelling in the perineal area and a history of falling from a height in the groin area 5 months before. The patient complains of the difficulty of urinating and hematuria one month after falling. We underwent drainage of the swelling area, found pus 100cc and took a sample for pathological examination. The result was embryonal rhabdomyosarcoma. Prostate volume was 122cc, PSA 5,32 and PSAD 0,04. The CT scan result was solid mass size 15x8x18 cm at perineum enhance to the pelvic cavity, push the bladder to the superior, rectum to posterior, and urethra posterior to the left side. We diagnosed this patient as Embryonal Rhabdomyosarcoma prostate T2bG1N0M0 (stage 3) group 3 and intermediate-risk group. The patient underwent VAC chemotherapy based on D.9803 (IRS V) protocol and planned radiotherapy, but it stopped at halfway because of profuse bleeding. Embryonal Rhabdomyosarcoma is an aggressive tumor, especially in adults. However, there was limited evidence and guideline to diagnose and manage the disease.
The Improvement of Community Knowledge, Attitudes and Practices After Covid-19 Socialization
Authors: Khaerunnisa Siti , Syafa'ah Irmi , Wungu Citrawati Dyah Kencono , Prabowo Gwenny Ichsan , Handajani Retno , Safitri Indri , Notopuro Harianto , Qurnianingsih Ema , Lukitasari Lina , Humairah Ira , Bakhtiar Arief , Suwandito Suwandito , Asih Susi Wahyuning , Anggraeni Zuhrotul Eka Yulis , Adi Ginanjar Sasmito , Nugrahani Ely Rahmatika , Zulka Ayesie Natasha , Soetjipto Soetjipto 
Published: July 2021

This study determined community knowledge, attitudes, and practices after COVID-19 socialization in Rambipuji and Suci Village, Jember District, East Java, Indonesia. This study used the analytic observational design study. As many as 40 people were given socialization about COVID-19 by gathering and online. The questionnaires were completed in two parts included before-socialization and after-socialization. The questionnaire data were analyzed descriptively by calculating frequency, percentage, and inferential statistics by t-test, Spearman correlation, and chi-square test. The data analysis used IBM SPSS version 23 software. The mean and standard deviation of percentage of knowledge pre-test, knowledge post-test, high attitude, moderate attitude, low attitude, very high practice, high practice, sufficient practice, and low practice were 58.33 ± 30.97, 77.70 ± 22.52, 62.5±21.7, 31±22.2, 6.5±17.1, 65±13.8, 32.3±13.8, 1.25±2.4, 1.25±1.8, respectively. Knowledge pre-test and post-test correlated 0.819 (p=0.001) and a t-test with p=0.003. Attitudes and practices correlated with p=0.001. Socialization of COVID-19 was useful to improve the community knowledge, attitudes, and practices in Rambipuji and Suci Village, Jember District, East Java, Indonesia that could prevent the transmission and inhibit the spread of the COVID-19 pandemic in Indonesia. Furthermore, continuous encouragement of COVID-19 socialization in wide areas was recommended.
Arab Saudi dan Iran: Kontestasi Ideologi dan Dampaknya di Kawasan Timur Tengah Pasca The Arab Spring
Authors: Hamid Ihsan , Setiadi Ozi 
Published: July 2021

<p class="06IsiAbstrak">Saudi Arabia and Iran: Ideological Contest and Its Impact in the Middle East Region after The Arab Spring. Tensions surround the Middle East region, especially between Saudi Arabia and Iran. The Sunni-Shia ideological factor contributed to the conflict in the region and also attracted these two countries. This research will answer the question how is the form of contestation of Sunni-Shia ideology represented by Saudi Arabia with Iran in the Middle East? What are the factors and impacts of the conflict between Saudi Arabia and Iran on countries in the region after the Arab Spring? The historical approach (historical analysis) was chosen to explain the two research questions above, using qualitative methods. Saudi Arabia and Iran represent both a Sunni-Shia ideological contestation, both of which try to support each other's ruling government, as well as conflicting oppositions based on ideological backgrounds. This support is carried out in the form of military assistance, weapons, or direct involvement which is marked by various statements that appear in the media. The disharmony of Saudi Arabia and Iran is driven by several factors, namely first, the Sunni-Shia ideological struggle between the two. Second, the claim to be the holder of the Islamic religion in each version. And third, oil. While the impact of this is political instability in the Middle East region after the Arab Spring, to the "cold war" involving Saudi Arabia and Iran. </p><p class="05Abstrak">.</p>
Severe Talonavicular Osteoarthritis After a Neglected Navicular Stress Fracture
Authors: Morio Hidenori , Sugimoto Kazuya , Isomoto Shinji , Nakamoto Yusuke , Samoto Norihiro , Tanaka Yasuhito 
Published: July 2021

Case:
A 17-year-old high school rugby player complained of right midfoot pain for 18 months. Radiographs showed severe osteoarthritic changes in the right talonavicular joint. A navicular fracture, talonavicular joint narrowing, a talar head cyst, and proximal navicular fragmentation were detected on computed tomography (CT). The patient underwent removal of the osteochondral fragments and fracture reduction and returned to rugby 8 months postoperatively. CT showed a remodeling of the talonavicular joint 2 years postoperatively.

Conclusion:
In a young athlete, reconstruction of the talonavicular joint should be attempted before joint fusion, even if the joint shows signs of secondary osteoarthritis.
Arterial Fat Embolism in a Pediatric Patient with Femur Fracture
Authors: Toto Regina , Friedlaender Eron Y. , Nace Gary W. , Arkader Alexandre , Levin L. Scott 
Published: July 2021

Case:
Here, we present the case of a pediatric polytrauma patient found to have lower extremity intra-arterial fat embolism causing ischemic necrosis and ultimately necessitating below-the-knee amputation.

Conclusion:
Fat embolism, a common complication of long bone fractures in adults, can be associated with significant morbidity. Although rare, it should be considered among the possible etiologies for a pulseless limb after trauma. Early fracture stabilization may prevent fat embolism and fat embolism syndrome; however, there is no known definitive treatment, and management is supportive.
Use of an Intraoperative Limb Positioner for Adjustable Distraction in Acetabulum Fractures with Femoral Head Protrusion
Authors: Goodnough Lawrence Henry , Olsen Thomas , Hidden Krystin , DeBaun Malcolm R. , Kleweno Conor P. 
Published: July 2021

Case:
Anatomic reduction of acetabular fractures with femoral head protrusion requires lateralization of the medialized femoral head to facilitate reduction and definitive fixation. In this case of a 71-year-old man with an associated both column acetabular fracture with femoral head medialization after a fall from a 10 foot ladder, we present the successful use of a novel reduction technique involving a modified arthroscopic limb positioner to provide adjustable distraction and counteract the deforming force in this injury pattern.

Conclusion:
A limb positioner can provide appropriate on-table traction in pelvis and acetabular surgery to neutralize deforming forces and facilitate reduction and fixation.
Molecular Characterization of the Highest Risk Adult Patients with Acute Myeloid Leukemia (AML) through Multi-omics Clustering
Authors: Nguyen Trinh , Pepper John W , Nguyen Cu , Fan Yu , Hu Ying , Chen Qingrong , Yan Chunhua , Meerzaman Daoud 
Published: July 2021

Abstract
Background: Acute myeloid leukemia (AML) is a clinically heterogeneous group of diseases with poor outcomes that are partly due to its complex and poorly understood heterogeneity. Methods: Here, we use a multi-omics approach to identify a molecular subgroup with the worst response to chemotherapy, and to identify promising drug targets specifically for this AML subgroup. Results: Multi-omics clustering analysis using RNA and CNA expression data resulted in the three primary clusters of 166 AML adult TCGA cancer cases. One of these clusters, which we label as the high-risk molecular subgroup (HRMS), consisted of cases that responded very poorly to standard chemotherapy, with only about 10% survival to two years. The gene TP53 was mutated in most cases in the HRMS, but not in other cases. The top 6 genes over-expressed in the HRMS included E2F4, CD34, CD109, MN1, MMLT3, and CD200. Multi-omics pathway analysis using RNA and CNA expression data identified in the HRMS subgroup over-expressed pathways related to immune function, cell proliferation, and DNA damage. Conclusion: Some AML patients are not successfully treated with the current standard of care chemotherapy, and urgently need targeted therapeutics. Potential drug targets include over-expressed genes E2F4, and MN1, as well as mutations in TP53, and several molecular pathways.
The Preventive and Treatment of the Neuroinflammasome in Sorokdo National Hospital
Authors: Lee Jong-Hoon , Lee Chul Joong , Lee So Jeong , Choi Su-Hee , Oh Sang-Suk , Park Jungwuk 
Published: July 2021

Abstract
Objective: This study investigated leprosy patients with Alzheimer's disease (AD) treated with 4,4’-diaminodiphenyl sulfone (DDS, Dapsone) as a neuroinflammasome competitor. Method: We searched all the Sorokdo National Hospital medical records and the National Health Insurance Service in South Korea and when the Korean government computerized the International Classification of Diseases (ICD)-9 code and Electronic Data Interchange (EDI). The Seoul study analyzed AD, and anti-AD drugs in the Sorokdo National Hospital's EDI database archived from January 2005 to June 2020 through the ICD-9 and -10 codes with National Health Insurance System. Result: The relation between DDS and AD each year was analyzed using the chi-square tests. P-values from 2007 to 2020 were significant. (P-value < 0.05) Treatment effects were proved with the T-Test Calculator. Four groups were defined: Treatment (T) 1: DDS prescription (+) AD prevalence (+), T 2: DDS (+) AD non-prevalence (-), T 3: DDS non-prescription (-) AD (+), T 4: DDS (-) AD (-). The results are all significant at p < .05. The participants with AD (T1) who took the DDS intervention (Mean (M) = 0.1766, Standard Deviation (SD) = 0.074) compared to the participants without AD (T2) who did not take the DDS in the control group (M = 0.5501, SD = 0.1421) demonstrated significantly lesser AD prevalence rates. The t-value is -8.72515 and the p-value is < .00001. The result is significant at p < 0.05.Conclusion: Our study is the first to prove dapsone as a preventive treatment for AD through observational studies for 15 years. This study demonstrated the neuroinflammasome therapy for AD.
A HER2-Target Antibody Drug Conjugate Combined with anti-PD-(L)1 Treatment Eliminates hHER2 + Tumors in hPD-1 Transgenic Mouse Model and Contributes Immune Memory Formation
Authors: Huang Lei , Wang Ruiqin , Xie Kun , Zhang jingming , Tao Fei , Pi Chenyu , Feng Yan , Fang Jianmin , Gu Hua 
Published: July 2021

Abstract
Purpose: Disitamab vedotin (RC48) is an HER2-directed antibody-drug conjugate, emerging as an effective strategy for cancer therapy, not only enhance antitumor immunity in previous animal models but also improve clinical outcomes for patients such as with gastric cancer, urothelium carcinoma and HER2 low-expressing breast cancer. Here, we explore the combination therapeutic efficacy of this novel HER2-targeting ADC with immune checkpoint inhibitors in a human HER2-expressing syngeneic breast cancer model. Methods: The human HER2+ cancer cell line is constructed by stably transfection and individual clones were isolated by single-cell sorting. Flow cytometry was performed to determine its binding activity. Cytotoxic effect was determined using an MTT assay with the supplement of RC48. Human PD-1 transgenic mice were used to analyze the in vivo anti-tumor effects of the ADC and its combination therapy with PD-1/PD-L1 antibody. Results: The combination of RC48 and PD-1/PD-L1 immune checkpoint inhibition significantly enhanced tumor suppression and antitumor immunity. Tumor rejection in the synergistic groups was accompanied by massive T-cell infiltration and immune marker activation. Furthermore, the combination therapy promoted immunological memory formation in the tumor-eradication animals, protecting them from tumor rechallenge. Conclusion: A novel HER2-targeting ADC combined with immune checkpoint inhibitors can achieve remarkable effects in mice and elicit long-lasting immune protection in a hHER2+ murine breast cancer model. This study provides insights into the efficacy of RC48 therapeutic activity and a rationale for potential therapeutic combination strategies with immunotherapy.
Molecular Typing and Epidemiology Profiles of Human Metapneumovirus Infection Among Children With Severe Acute Respiratory Infection in Huzhou, China
Authors: Ji Lei , Chen Liping , Xu Deshun , Wu Xiaofang 
Published: July 2021

Abstract
Background: Human metapneumovirus (hMPV) is one of the important pathogens of infant respiratory tract infection. However, the molecular epidemiological information relating to hMPV among children hospitalized patients with severe acute respiratory infection (SARI) have not been thoroughly studied as yet. To investigate the infection status and genotypes of hMPV among children hospitalized patients with SARI from January 2016 to December 2020 in Huzhou, China. Methods: A nasopharyngeal flocked swab, nasal wash or combination of nasopharyngeal swab and oropharyngeal swab samples were collected from children with SARI in Huzhou from January 2016 to December 2020. RT-PCR was used to detect the nucleic acid of hMPV. F gene was amplified and sequenced for the positive nucleic acid samples of hMPV. The obtained gene sequences were analyzed by MEGA software (version 7.0). Epidemiological data were analyzed using Microsoft Excel 2010 and service solutions (SPSS) 21.0 software.Results: A total of 1133 children with SARI were collected from 2016 to 2020. Among them, 56 cases were positive for hMPV nucleic acid, with a positive rate of 4.94%. Children under 5 years old accounted for 85.71% of the total positive cases. Higher activity of hMPV infection could be seen in the period in Spring and Winter. and the main epidemic months were December and January-March. The F gene sequences of 28 strains of hMPV were obtained by co-sequencing. Phylogenetic analysis showed that there were A1, B1 and B2 genotypes of hMPV prevalent in Huzhou, and the dominant genotype of hMPV during our study period varied according to surveillance year.Conclusions: HMPV is one of the important pathogens causing acute respiratory virus infection in children in Huzhou, with high incidence in winter and spring seasons and children under the age of 5，A1, B1 and B2 are three prevalent genotypes.
Anti-Metastatic Effect of GV1001 on Prostate Cancer Cells; Roles of GnRHR-Mediated Gαs-cAMP Pathway and AR-YAP1 Axis
Authors: Kim Ji Won , Park Miso , Kim Suntae , Lim Sung Chul , Kim Hyung Shik , Kang Keon Wook 
Published: July 2021

Abstract
Background: Gonadotropin-releasing hormone receptor (GnRHR) transmits its signal via two major Gα-proteins, primarily Gαq and Gαi. However, the precise mechanism underlying the functions of Gαs signal in prostate cancer cells is still unclear. We have previously identified that GV1001, a fragment of the human telomerase reverse transcriptase, functions as a biased GnRHR ligand to selectively stimulate the Gas/cAMP pathway. Here, we tried to reveal the potential mechanisms of which GV1001- stimulated Gαs-cAMP signaling pathway reduces the migration and metastasis of prostate cancer (PCa) cells.Methods: The expression of epithelial-mesenchymal transition (EMT)-related genes was measured by western-blotting and spheroid formation on ultra-low attachment plate was detected after GV1001 treatment. In vivo Spleen-liver metastasis mouse model was used to explore the inhibitory effect of GV1001 on metastatic ability of PCa and the transwell migration assay was performed to identify whether GV1001 had a suppressive effect on cell migration in vitro. In order to demonstrate the interaction between androgen receptor (AR) and YAP1, co-immunoprecipitation (co-IP), immunofluorescence (IF) staining, chromatin immunoprecipitation (ChIP) were performed in LNCaP cells with and without GV1001 treatment.Results: GV1001 inhibited expression of EMT-related genes and spheroid formation. GV1001 also suppressed in vivo spleen-liver metastasis of LNCaP cells as well as cell migration in vitro. GV1001 enhanced the phosphorylation of AR and transcription activity of androgen response element reporter gene through cAMP/protein kinase A pathway. Moreover, GV1001 increased Ser-127 phosphorylation of YAP1 and its ubiquitination, and subsequently decreased the levels of AR-YAP1 binding in the promoter region of the CTGF gene. In contrast, both protein and mRNA levels of NKX3.1 known for tumor suppressor gene and AR-coregulator were upregulated by GV1001 in LNCaP cells. YAP1 knockout using CRISPR/Cas9 significantly suppressed the migration ability of LNCaP cells, and GV1001 did not affect the cell migration of YAP1-deficient LNCaP cells. On the contrary, cell migration was more potentiated in LNCaP cells overexpressing YAP5SA, a constitutively active form of YAP1, which was not changed by GV1001 treatment. Conclusions: Overall, this study reveals an essential role of AR-YAP1 in the regulation of PCa cell migration, and provide evidence that GV1001 could be a novel GnRHR ligand to inhibit metastasis of PCa via the Gas/cAMP pathway.
Surface Water Quality Variation Under the Influence of COVID-19 Quarantine on the Example of the Yamuna River (Delhi)
Authors: Khan Nadeem A , Khan Afzal Husain , Mazhar Mohd. Aamir , Abdelgiom Mahmoud Abdelrahim , Yadav Krishna Kumar , Yadav Akanksha , Cabral-Pinto Marina M.S. , Yadav Shalini 
Published: July 2021

Abstract
During the current COVID-19 pandemic, various forms of lockdown have been adopted, globally. This study evaluated the contamination changes (pre, post and during the lockdown) in the Yamuna River following the nationwide COVID-19 lockdown from 25 March–30 May (India). Samples of the surface water were taken from 9 sampling points to determine the physico-chemical, and biological concentration changes in surface water. The investigation showed the fluctuating results of the parameters. The peak saturation of physico-chemical parameters were observed prior to lockdown, followed by the post and during lockdown phases. The BOD and COD concentrations declined by 66% and 39.25%, respectively, compared to the pre-lockdown phase, while Faecal Coliform declined by over 40%. The improvement shown in this period indicates that it is possible for the Yamuna River to be cleaned up easily if people and the government come together.
Synthesis And Characterization of Vancomycin-Loaded Chitosan Nanoparticles For Drug Delivery
Authors: Hafizi Tajuddin , Shahriari Mehrnoosh Hasan , Abdouss Majid , Kahdestani Shafiq Ahmad 
Published: July 2021

Abstract
Chitosan is a linear polysaccharide with and prominent physicochemical and biological properties such as biocompatibility, biodegradability, nontoxicity, non-immunogenicity, bioadhesion, antibacterial, antifungal and hemostatic activity. Due to these properties, it has found many applications in cosmetic, textile, and food industries; agriculture; biotechnology; pharmaceutical industry and medicine; specially in biomedical applications. The special chemical structure of chitosan allows some specific modifications and by reducing the size of chitosan particles to Nano size, it becomes an excellent drug nanocarrier. Vancomycin is a typical antibiotic used for bacterial infections caused by geram positive bacteria. In this work, chitosan nanoparticles (CSNPs) were prepared via ionotropic gelation using tripolyphosphate (TPP) as crosslinker. The effect of chitosan and TPP concentration on the size of chitosan nanoparticles was studied and CS:TPP ratio of 1:1 with average size of nanoparticle about 100 nm were selected. The prepared samples were characterized using DLS, FTIR, TGA, DSC, and SEM techniques. The results confirmed that vancomycin has been loaded successfully on chitosan nanoparticles and there was not any interaction between vancomycin and chitosan. Also, it is observed that 40% of vancomycin is released burstly in the first 9 h and after that the drug release is continued gradually to receive 90% at 100 h.
Pseudaestuariivita rosea sp. nov., a novel species of genus Pseudaestuariivita, isolated from Acmaea, a kind of marine mollusea
Authors: Yun Shuai-Ting , Chen Zhuo , Yi Yan-Jun , Zhang Ming-Jing , Gao Shu-Kun , Zhou Yan-Xia 
Published: July 2021

Abstract
A Gram-stain-negative, pink-pigmented, facultatively anaerobic, gliding and rod-shaped bacterium, showing optimum growth at 33°C, designated as strain H15T , was isolated from the gut microbial of the Acmaea from Weihai, Shandong Province, China and characterised phylogenetically, phenotypically and chemotaxonomically. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the strain belonged to the family Rhodobacteraceae and was associated with members of the recognized genera, the closest relative was the type strain of Pseudaestuariivita atlantica (96.7%). Genome analysis showed that the genome size was 3893398 bp and the DNA G+C content obtained from the draft genome sequence was 56.7%. The secondary metabolites based on genome predicated that the strain H15T contained one cluster of lassopeptide, one cluster of bacteriocin, two clusters of terpene production, two clusters of homoserine lactone(Hserlactone)production and one cluster of beta lactone. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values between genome sequences of strain H15T and all the type strains of the recognized taxa compared were lower than 63.1,72.0 and 19.7%, respectively. Based on the analysis of chemical components, the predominant cellular fatty acids were summed featured 8(C18:1ω7c/ω6c,46.1%), C 20:1ω7c (17.1%), the major polar lipids contained phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid and the predominant menaquinone was Q10. Therefore, the combined chemotaxonomic, phenotypic and phylogenetic data indicated that the strain was considered to represent a novel species of the genus Pseudaestuariivita and the name Pseudaestuariivita rosea sp. nov. was proposed for strain H15T(MCCC 1K04420T=KCTC 82505T).