bluuebunny/crossref_metadata_2025_split huggingface dataset:
A tandem duplication in Drosophila melanogaster shows enhanced expression beyond the gene copy number
Authors: Loehlin David W , Kim Jeremiah Y , Paster Caleigh O 
Published: December 2021

Abstract
Tandem duplicated genes are common features of genomes, but the phenotypic consequences of their origins are not well understood. It is not known whether a simple doubling of gene expression should be expected, or else some other expression outcome. This study describes an experimental framework using engineered deletions to assess any contribution of locally acting cis- and globally acting trans-regulatory factors to expression interactions of particular tandem duplicated genes. Acsx1L (CG6300) and Acsx1R (CG11659) are tandem duplicates of a putative acyl-CoA synthetase gene found in Drosophila melanogaster. Experimental deletions of the duplicated segments were used to investigate whether the presence of 1 tandem duplicated block influences the expression of its neighbor. Acsx1L, the gene in the left block, shows much higher expression than either its duplicate Acsx1R or the single Acsx1 in Drosophila simulans. Acsx1L expression decreases drastically upon deleting the right-hand duplicated block. Crosses among wildtype and deletion strains show that high tandem expression is primarily due to cis-acting interactions between the duplicated blocks. No effect of these genes on cuticular hydrocarbons was detected. Sequence and phylogenetic analysis suggest that the duplication rose to fixation in D. melanogaster and has been subject to extensive gene conversion. Some strains actually carry 3 tandem copies, yet strains with 3 Acsx1s do not have higher expression levels than strains with 2. Surveys of tandem duplicate expression have typically not found the expected 2-fold increase in expression. This study suggests that cis-regulatory interactions between duplicated blocks could be responsible for this trend.
The Impact of Marital Quality as Older Couples Adjust to Dementia Onset
Authors: Huo Meng , Kim Kyungmin , Han Sae Hwang 
Published: December 2021

Abstract

Objectives
A burgeoning literature links being married to better cognitive health, but less attention has been paid to how couples view their marital relationships. Couples do not always concur in their assessments, and such discrepancies affect both partners’ health. We present a dyadic study on whether and how overall and discrepant views of marital quality predicted (a) dementia onset and (b) changes in older adults’ depressive symptoms with spousal dementia.

Methods
A pooled sample of couples aged 50+ (dyad N = 3,936) from the Health and Retirement Study rated positive and negative marital quality at baseline (2006/2008). Each participant reported whether they had been told of having dementia and their depressive symptoms once every other year (2006/2008–2014/2016).

Results
Cox proportional hazards regression revealed that older adults who rated their marriages either more positively or more negatively than their spouses were more likely to develop dementia. We applied multiphase growth curve modeling to older adults whose spouses developed dementia, finding that those in marriages that were more negative overall reported more depressive symptoms but exhibited a smaller increase in these symptoms in response to spousal dementia.

Discussion
This study adds to the literature by showing how discrepant marital assessments shape cognitive aging and offers new insights into identifying older adults with greater dementia risk. Findings also revealed the impact of overall negative marital quality on older adults’ psychological adjustment to spousal dementia, which could inform interventions intended to help couples better cope with early-stage dementia from a relational perspective.
Dementia Family Caregiving in Rural Appalachia: A Sociocultural Model of Care Decisions and Service Use
Authors: Roberto Karen A , Savla Jyoti , McCann Brandy Renee , Blieszner Rosemary , Knight Aubrey L 
Published: December 2021

Abstract

Objectives
Examinations of cultural variations in caregiving experiences and practices tend to focus on racially and ethnically diverse family caregivers. We extended this research by exploring the role of place-based cultural constructs on care decisions of family caregivers for persons living with dementia in rural Appalachia whose experiences and backgrounds dwell outside the lines of dominant culture.

Methods
Guided by our sociocultural conceptual model of service use and a descriptive qualitative study design, we conducted in-depth interviews with 30 caregivers for persons living with dementia to understand how predisposing and enabling factors influence caregiving decisions, including the use of services to care for persons living with dementia.

Results
Three predisposing factors reflecting the culture of rural Appalachian counties in Virginia (reasons for becoming a caregiver, previous caregiving experience, and deference to the person living with dementia) shaped decisions about caregiving responsibilities and service use. Three place-based enabling factors (a personal sense of belonging, knowledge of services, and family support for service use) played an important role in facilitating access to services.

Discussion
Findings revealed subtle variation across caregivers in this study and in comparison, to caregivers’ experiences more generally. We interpret these variations in light of culturally influenced attitudes, family norms, and sources of information about formal services. Place-based cultural experience influenced dementia family caregivers’ behaviors and choices regarding formal service use, suggesting that investigators should pay more attention to cultural influences in future caregiving research.
Vaccine hesitancy and acceptance: an examination of predictive factors in COVID-19 vaccination in Saudi Arabia
Authors: Fadhel Fahmi Hassan 
Published: December 2021

Summary
Vaccine hesitancy is a global health issue and can be affected by several variables. We explored the predictive factors and causes of vaccine hesitancy among adults in Saudi Arabia. An online survey method with multiple regression analysis was used to identify factors predicting of vaccine hesitancy in 558 adults (46.24% women and 53.76% men). The prevalence of vaccine hesitancy is 20.6%, with higher rates among females, young people and single people. About 70% of the participants believe that vaccine hesitancy is due to concerns about the safety and efficacy of the vaccine, a lack of information about the disease and vaccine or social media. The vaccine acceptance rate is 71.3%; 17.2% are not willing to accept a COVID-19 vaccine and 11.5% are unsure. Males and married people are more accepting of the vaccine. The risk factors that predict vaccine hesitancy include age, gender, belief in conspiracy theories and psychosocial factors. Meanwhile, age, gender, belief in conspiracy theories, concerns about the safety and efficacy of the vaccine and psychosocial factors significantly predict vaccine acceptance. The high rate of vaccine hesitancy could undermine efforts to combat COVID-19. Factors predicting vaccine hesitancy can be used in interventions to address this issue during major epidemics.
Modified use of a 4-branched frozen elephant trunk prosthesis for treatment of a right-sided aortic arch aneurysm with an aberrant left subclavian artery
Authors: Brickwedel Jens , Reichenspurner Hermann , Kölbel Tilo , Detter Christian 
Published: December 2021

Abstract
Right aortic arch aneurysm originating from a Kommerell’s diverticulum associated with an aberrant left subclavian artery is a rare congenital entity. We report a case of an asymptomatic 60-year-old female with right aortic arch aneurysm with an aberrant left subclavian artery, treated with a modified frozen elephant trunk technique using a 4-branched prosthesis, with the perfusion branch as an extra-anatomical bypass to the aberrant left subclavian artery. This case demonstrates short-term safety and efficacy of this technique.
Vaccine Effectiveness Against Influenza Hospitalization and Emergency Department Visits in 2 A(H3N2) Dominant Influenza Seasons Among Children &lt;18 Years Old—New Vaccine Surveillance Network 2016–2017 and 2017–2018
Authors: Kim Sara S , Naioti Eric A , Halasa Natasha B , Stewart Laura S , Williams John V , Michaels Marian G , Selvarangan Rangaraj , Harrison Christopher J , Staat Mary A , Schlaudecker Elizabeth P , Weinberg Geoffrey A , Szilagyi Peter G , Boom Julie A , Sahni Leila C , Englund Janet A , Klein Eileen J , Ogokeh Constance E , Campbell Angela P , Patel Manish M ,   New Vaccine Surveillance Network, Hickey Robert W , Piedra Pedro A , Munoz Flor M , Avadhanula Vasanthi , Schuster Jennifer E , Pahud Barbara A , Weddle Gina , Moffatt Mary E , Albertin Christina , Fregoe Wende , McNeal Monica 
Published: December 2021

Abstract
Studies have shown egg-adaptive mutations in influenza vaccine strains that might have impaired protection against circulating A(H3N2) influenza viruses during the 2016–2017 and 2017–2018 seasons. We used the test-negative design and multivariable models to assess vaccine effectiveness against influenza-associated hospitalization and emergency department visits among children (&lt;18 years old) during the 2016–2017 and 2017–2018 seasons. Effectiveness was 71% (95% confidence interval, 59%–79%), 46% (35%–55%), and 45% (33%–55%) against A(H1N1)pdm09, A(H3N2), and B viruses respectively, across both seasons. During high-severity seasons with concerns for vaccine mismatch, vaccination offered substantial protection against severe influenza outcomes requiring hospitalization or emergency department visits among children.
Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma
Authors: Park Keon Young , Hefti Hunter O , Liu Peng , Lugo-Cintrón Karina M , Kerr Sheena C , Beebe David J 
Published: December 2021

Abstract
Renal cell carcinoma (RCC) is the third most common genitourinary cancer in the USA. Despite recent advances in the treatment for advanced and metastatic clear cell RCC (ccRCC), the 5-year relative survival rate for the distant disease remains at 12%. Cabozantinib, a tyrosine kinase inhibitor (TKI), which is one of the first-line therapies approved to treat advanced ccRCC as a single agent, is now being investigated as a combination therapy with newer immunotherapeutic agents. However, not much is known about how cabozantinib modulates the immune system. Here, we present a high throughput tri-culture model that incorporates cancer cells, endothelial cells, and patient-derived immune cells to study the effect of immune cells from patients with ccRCC on angiogenesis and cabozantinib resistance. We show that circulating immune cells from patients with ccRCC induce cabozantinib resistance via increased secretion of a set of pro-angiogenic factors. Using multivariate partial least square regression modeling, we identified CD4+ T cell subsets that are correlated with cabozantinib resistance and report the changes in the frequency of these populations in ccRCC patients who are undergoing cabozantinib therapy. These findings provide a potential set of biomarkers that should be further investigated in the current TKI-immunotherapy combination clinical trials to improve personalized treatments for patients with ccRCC.
A quantitative view of strategies to engineer cell-selective ligand binding
Authors: Tan Zhixin Cyrillus , Orcutt-Jahns Brian T , Meyer Aaron S 
Published: December 2021

Abstract
A critical property of many therapies is their selective binding to target populations. Exceptional specificity can arise from high-affinity binding to surface targets expressed exclusively on target cell types. In many cases, however, therapeutic targets are only expressed at subtly different levels relative to off-target cells. More complex binding strategies have been developed to overcome this limitation, including multi-specific and multivalent molecules, creating a combinatorial explosion of design possibilities. Guiding strategies for developing cell-specific binding are critical to employ these tools. Here, we employ a uniquely general multivalent binding model to dissect multi-ligand and multi-receptor interactions. This model allows us to analyze and explore a series of mechanisms to engineer cell selectivity, including mixtures of molecules, affinity adjustments, valency changes, multi-specific molecules and ligand competition. Each of these strategies can optimize selectivity in distinct cases, leading to enhanced selectivity when employed together. The proposed model, therefore, provides a comprehensive toolkit for the model-driven design of selectively binding therapies.
Assessing the prevalence of refractive errors and accuracy of vision screening by schoolteachers in Liberia
Authors: Tobi Patrick , Ibrahim Nazaradden , Bedell Alex , Khan Imran , Jolley Emma , Schmidt Elena 
Published: December 2021

AbstractBackgroundEvidence indicates that school-based vision screening by trained teachers is an effective way of identifying and addressing potential vision problems in schoolchildren. However, inconsistencies have been reported in both the testing methods and accuracy of the screeners. This study assessed the prevalence of refractive errors and accuracy of screening by teachers in Grand Kru County, Liberia.MethodsWe conducted a retrospective analysis of data from four schools where, in February 2019, children were screened for refractive errors by trained teachers and then re-examined by ophthalmic technicians. One row of five optotypes of the Snellen 6/9 (0.2 logMar) scale (tumbling E chart) was used at a distance of 3 m. The prevalence of visual impairment and associations with sex, age and school were explored. Sensitivity, specificity and predictive values were calculated.ResultsData were available for 823 of 1095 eligible children with a mean age of 13.7 y (range 5–18) and male:female ratio of 1:0.8. Poor vision was identified in 24 (2.9%) children with no differences by either sex or age but small differences by school. Screening by teachers had a sensitivity of 0.25 (95% confidence interval [CI] 0.077 to 0.423) and a specificity of 0.996 (95% CI 0.992 to 1.000). Positive and negative predictive values were 0.667 (95% CI 0.359 to 0.975) and 0.978 (95% CI 0.968 to 0.988), respectively. The results were influenced by a high number of misclassifications in one of the four schools.ConclusionsTeachers can be trained to conduct vision screening tests on schoolchildren to an acceptable level of accuracy, but strong monitoring and quality assurance systems should be built into screening programmes from the onset. In settings like Liberia, where many children do not attend school regularly, screening programmes should extend to community platforms to reach children out of school.
Using mystery shoppers to determine practices pertaining to antibiotic dispensing without a prescription among community pharmacies in South Africa—a pilot survey
Authors: Mokwele R. Nelly , Schellack Natalie , Bronkhorst Elmien , Brink Adrian J. , Schweickerdt Louise , Godman Brian 
Published: December 2021

Abstract

Background
Inappropriate use of antimicrobials is a key factor increasing antimicrobial resistance, a major global public health problem including in South Africa. Key drivers include antibiotics being dispensed without a prescription.

Objectives
To determine the accessibility of antibiotics without a prescription in community pharmacies in urban areas in South Africa and determine whether counselling was provided when antibiotics were dispensed.

Patients and methods
Prospective, observational study, employing simulated patients (SPs), presenting with upper respiratory tract infections (URTIs) and urinary tract infections (UTIs), undertaken to establish whether antibiotics can be obtained without a valid prescription in South Africa. This pilot study was conducted in privately owned (n = 20) and corporate (franchised, n = 14) community pharmacies in three regions in Gauteng Province.

Results
Antibiotics were sold in privately owned pharmacies without a prescription in 80% (16/20) of cases while no antibiotics were dispensed in corporate (franchised) pharmacies. Of the 16 pharmacies selling antibiotics without a prescription, pharmacist assistants were involved in 37.5% (n = 6) and counselling was not provided to 19% of SPs. Ciprofloxacin (42.9%) and metronidazole (28.6%) were the most common antibiotics dispensed. No antibiotics were dispensed for URTIs, only UTIs.

Conclusions
Dispensing antibiotics without prescriptions can be common among privately owned pharmacies in urban areas in South Africa. Corporate pharmacies, which probably have a greater income, appear to follow current legislation banning such activities. To limit selling with no prescription, community pharmacists and assistants especially in urban areas should be educated on appropriate patient care and legal requirements, with dispensing electronically monitored.
Analysis of Fragrance Allergens in Personal Care Products, Toys, and Water Samples: A Review
Authors: Chen Jing , Yi Zonghui , Sun Rui , Ning Wei , Zhou Chen , Tian Ziyi , Sun Chengjun , Li Yongxin 
Published: December 2021

Abstract
Fragrance allergens (FAs) refer to these volatile or semi-volatile fragrance compounds that can cause irritation and negative reactions. A large number of emerging FAs are widely used in household goods, and cause contact allergy or allergic contact dermatitis in eczema population and the general population. It shows an increasing prevalence and is regarded as a concern to public health. Recently, more and more studies have focused on the analytical methods of FAs in a variety of samples with different matrixes. Therefore, a systematic and comprehensive overview of recent progress of analysis of FAs in various samples is needed. In this review, the physical and chemical properties, applications, hazards, and the recent advances of sample preparation and determination methods of common FAs in personal care products, toys, and water samples are systematically and comprehensively summarized. Meanwhile, this review also discusses the advantages and limitations of different sample pretreatment and detection methods, thus offering a deep-going discussion of the development and future trends in this area
Determination of Heavy Metals in a Variety of Cannabis and Cannabis-Derived Products, First Action 2021.03
Authors: Nelson Jenny , Jones Craig , Heckle Sam , Anderson Leanne 
Published: December 2021

Abstract

Background
The legalization of medicinal or recreational marijuana across many states in the United States and other countries has driven demand for cannabis, hemp, and related products.

Objective
In the absence of federal regulations to ensure the product quality and safety of these products, each state issues its own guidance and sets its own regulations. Like food and pharmaceuticals, cannabis testing should include the analysis of heavy metals, which may be toxic if ingested or inhaled.

Methods
Based on established methods for the preparation and multi-elemental analysis of plant materials, a range of cannabis and cannabis-related products were prepared for analysis using microwave-assisted acid digestion followed by testing with inductively coupled plasma-mass spectrometry (ICP-MS). The sample preparation procedure was validated by measuring arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) in four plant-based certified reference materials (CRMs).

Results
The mean ICP-MS results for As, Cd, Hg, and Pb were in good agreement (85–110%) with the certified concentrations. The accuracy and precision of the ICP-MS method for the determination of As, Cd, Hg, and Pb, as well as other elements, were tested by spiking the various cannabis samples at different concentration levels and determining the spike recoveries. The spike recoveries for As, Cd, Hg, and Pb in all spiked samples met the AOAC Standard Method Performance Requirements (SMPR®) for Determination of Heavy Metals in a Variety of Cannabis and Cannabis-Derived Products (SMPR 2020.001) of 60–115% for low-level spike and 80–115% for mid- and high-level spikes.

Conclusion
Microwave-assisted acid digestion and ICP-MS are suitable for trace metal preparation and analysis of cannabis, as well as related products, as shown by the results of this method. The repeatability and recovery results for As, Cd, Hg, and Pb met the method requirement criteria in the AOAC SMPR 2020.001. This method can be used for all stages of production to ensure safety with respect to toxic metals.

Highlights
A wide range of cannabis and hemp samples, from raw materials to finished products, are within scope for analysis using the microwave-assisted acid digestion/ICP-MS method.
Pre-hatching and post-hatching environmental factors related to epigenetic mechanisms in poultry
Authors: Dunislawska Aleksandra , Pietrzak Elzbieta , Wishna Kadawarage Ramesha , Beldowska Aleksandra , Siwek Maria 
Published: December 2021

Abstract
Epigenetic modifications are phenotypic changes unrelated to the modification of the DNA sequence. These modifications are essential for regulating cellular differentiation and organism development. In this case, epigenetics controls how the animal’s genetic potential is used. The main epigenetic mechanisms are microRNA activity, DNA methylation, and histone modification. The literature has repeatedly shown that environmental modulation has a significant influence on the regulation of epigenetic mechanisms in poultry. The aim of this review is to give an overview of the current state of the knowledge in poultry epigenetics in terms of issues relevant to overall poultry production and the improvement of the health status in chickens and other poultry species. One of the main differences between birds and mammals is the stage of embryonic development. The bird’s embryo develops outside its mother, so an optimal environment of egg incubation before hatching is crucial for development. It is also the moment when many factors influence the activation of epigenetic mechanisms, i.e., incubation temperature, humidity, light, as well as in ovo treatments. Epigenome of the adult birds might be modulated by nutrition, supplementation, and treatment, as well as modification of the intestinal microbiota. In addition, the activation of epigenetic mechanisms is influenced by pathogens (i.e., pathogenic bacteria, toxins, viruses, and fungi) as well as the maintenance conditions. Farm animal epigenetics is still a big challenge for scientists. This is a research area with many open questions. Modern methods of epigenetic analysis can serve both in the analysis of biological mechanisms and in the research and applied to production system, poultry health, and welfare.