bluuebunny/crossref_metadata_2025_split huggingface dataset:
Globo H Is a Promising Theranostic Marker for Intrahepatic Cholangiocarcinoma
Authors: Hung Tsai‐Hsien , Hung Jung‐Tung , Wu Chiao‐En , Huang Yenlin , Lee Chien‐Wei , Yeh Chau‐Ting , Chung Yi‐Hsiu , Lo Fei‐Yun , Lai Li‐Chun , Tung John K. , Yu John , Yeh Chun‐Nan , Yu Alice L. 
Published: August 2021

Recent studies support the development of cancer therapeutics to target Globo H‐ceramide, the most prevalent tumor‐associated carbohydrate antigen in epithelial cancers. Herein, we evaluated the expression of Globo H and its prognostic significance in intrahepatic cholangiocarcinoma (ICC) and conducted preclinical studies to assess the antitumor activity of Globo H–specific antibody in thioacetamide (TAA)–induced ICC in rats. Globo H–ceramide in tumor specimens was detected by immunohistochemistry (IHC) and mass spectrometry. Antitumor efficacy of anti–Globo H mAbVK9 was evaluated in TAA‐induced ICC in rat. Natural killer (NK) cells and their related genes were analyzed by IHC and quantitative real‐time polymerase chain reaction. Data mining revealed that B3GALT5 and FUT2, the key enzymes for Globo H biosynthesis, were significantly up‐regulated in human ICC. In addition, Globo H expression was detected in 41% (63 of 155) of ICC tumor specimens by IHC staining, and validated by mass spectrometric analysis of two IHC‐positive tumors. Patients with Globo H positive tumors had significantly shorter relapse‐free survival (RFS) and overall survival (P = 0.0003 and P = 0.002, respectively). Multivariable Cox regression analysis identified Globo H expression as an independent unfavorable predictor for RFS (hazard ratio: 1.66, 95% confidence interval: 1.08‐2.36, P = 0.02) in ICC. Furthermore, gradual emergence of Globo H in liver tissues over 6 months in TAA‐treated rats recapitulated the multistage progression of ICC in vivo. Importantly, administration of anti‐Globo H mAbVK9 in rats bearing TAA‐induced ICC significantly suppressed tumor growth with increased NK cells in the tumor microenvironment. Conclusion: Globo H is a theranostic marker in ICC.
Identification and functional analysis of the mitochondrial cysteine synthase TtCsa2 from Tetrahymena thermophila
Authors: Lv Hongrui , Hu Lina , Xu Jing , Bo Tao , Wang Wei 
Published: August 2021

AbstractCysteine is a crucial component for all organisms and plays a critical role in the structure, stability, and catalytic functions of many proteins. Tetrahymena has reverse transsulfuration and de novo pathways for cysteine biosynthesis. Cysteine synthase is involved in the de novo cysteine biosynthesis and catalyzes the production of cysteine from O‐acetylserine. The novel cysteine synthase TtCSA2 was identified from Tetrahymena thermophila. The TtCSA2 showed high expression levels at the log‐phase and the sexual development stage. The TtCsa2 was localized on the outer mitochondrial membrane throughout different developmental stages. However, the truncated N‐terminal signal peptide mutant TtCsa2‐ΔN23 was localized into the mitochondria. His‐TtCsa2 was expressed in Escherichia coli and purified using affinity chromatography. The His‐TtCsa2 showed O‐acetylserine sulfhydrylase and serine sulfhydrylase activities. Cysteine and glutathione contents decreased in the csa2KD mutant. Furthermore, mutant cells were sensitive to cadmium and copper stresses. This study indicated that the TtCSA2 was involved in the cysteine synthesis in mitochondria and related to heavy metal stresses resistance in Tetrahymena.
Evaluation of point‐of‐care testing device for anemia detection: A cross‐sectional method comparison study from Thailand
Authors: Chantkran Wittawat , Jamnarnwej Pitipat , Sritanabutr Pipat , Arnutti Pasra 
Published: August 2021

AbstractBackgroundA comparison study is crucial before launching a new medical device; therefore, we compared the Mission Ultra Hb Testing System with the Sysmex XN‐3000 automated hematology analyzer in Thai adult males and non‐pregnant adult females.MethodsParallel studies were conducted using discarded venous K2‐ethylenediaminetetraacetic acid samples from participants requiring hematological investigations. According to the World Health Organization criteria, the participants were categorized as overall, anemia, and non‐anemia for analysis.ResultsThree hundred participants were included in this study. In all participants, near‐perfect correlation and agreement were observed between the two methods for Hb measurement (r = 0.963, p < 0.001) with an interclass correlation coefficient (ICC) of 0.981 (95% confidence interval [CI]: 0.976–0.985) and Hct measurement (r = 0.941, p < 0.001) with an ICC of 0.965 (95% CI: 0.956–0.972). The sensitivity and specificity of the device in detecting anemia were 86.2% (95% CI: 79.7–91.2) and 98.6% (95% CI: 95.2–99.8), respectively. The area under the curve was 0.976 (95% CI: 0.963–0.989). The device showed average biases of 0.76 g/dl (95% limits of agreement [LOA]: −1.03 to 2.54) for Hb measurement and −2.73% (95% LOA: −9.28 to 3.82) for Hct measurement in all participants.ConclusionAgreement between the Mission Ultra Hb Testing System and Sysmex XN‐3000 was observed. The device was excellent for detecting anemia. However, the essential evidence showing biases of the Hb and Hct measurements obtained from the device was revealed. Laboratory interpretation should be carefully performed, particularly at the near cut‐off values.
Preventing Indigenous youth homelessness in Canada: A qualitative study on structural challenges and upstream prevention in education
Authors: Ansloos Jeffrey Paul , Wager Amanda Claudia , Dunn Nicole Santos 
Published: August 2021

AbstractDrawing on a partnership with a group of Indigenous youth experiencing homelessness in Vancouver, Canada, this study identifies four structural challenges that have impacted them and four actionable upstream strategies to further prevent youth housing precarity. As a secondary analysis of a community‐engaged study with youth experiencing homelessness, we conducted a thematic analysis with qualitative data, which included qualitative interviews with five young people and researcher observations. The results reflect the racial, colonial, and economic concerns that impact Indigenous youth experiencing homelessness. The four actionable upstream solutions highlight human rights‐based approaches to homelessness, ranging from advancing and strengthening public services, transitional justice processes, and cultural and socioeconomic safety. This study provides strategies to promote Indigenous youth wellbeing and decrease risk of housing precarity, while centering and drawing from youth knowledge production. Strengths and limitations of the study are also discussed.
Opportunities to improve behavioral health crisis response: Results of a large urban county's community status assessment
Authors: Flynn Karen Coen , Riske‐Morris Michelle , Hussey David L. 
Published: August 2021

AbstractA comprehensive community status assessment of an Ohio urban county's crisis response (CR) system explored the experiences of its behavioral health services' clients and providers to surface themes characterizing the system's responsiveness and identifying opportunities for improvements. Forty‐eight focus groups and two online surveys were conducted. Data were analyzed using qualitative content analysis and descriptive statistics. The greatest areas of needed improvement ascertained by this effort are in increased CR system resources, more efficient use of resources, and capacity enhancements in nine areas: the mobile crisis team, CR protocols, psychiatric inpatient and crisis stabilization beds, stabilization admission for eligible persons, stabilization services for in‐crisis but admission‐ineligible persons, continuity of care, research into child versus adult CR systems, Provider Emergency Support Program, and first responder crisis intervention training. The assessment provides a foundation for the county to identify further opportunities for system scale‐up.
Impact on reproductive performance and body condition in a small limpet parasitized by a large castrator pea crab
Authors: Ocampo Emiliano H. , Pérez García Macarena , Nuñez Jesús D. , Luppi Tomás A. 
Published: August 2021

AbstractParasitic castrators utilize the energy reserves that the host allocates on reproduction resulting in sterilization of the host. However, whether other processes (e.g., growth) are also impaired depends on the balance between the castrator's energy requirements and the available resources that the castrated host does not use in reproduction. We investigated whether the castrator pea crab Calytraeotheres garthi alters body condition, reproductive performance, and occurrence of copulation in its limpet host Bostrycapulus odites. For this purpose, we examined the anatomy of the ovary, the seminal receptacles, and the body condition of parasitized and unparasitized limpets. The histology of the female gonad of parasitized limpets revealed the ovarian tubules are smaller and characterized by a greater proportion of intertubular and intratubular free space compared with non‐parasitized individuals. The body condition of female limpets from all sizes (during summer) and those larger than ~16 mm (during spring and autumn) were impacted by the pea crab. These results are in contrast to that previously reported in the comparatively larger limpet species Crepidula cachimilla, in which the same pea crab species does not alter or even increase the host body weight. We concluded this pea crab species could drastically impair its host's reproduction and body condition although deleterious effects are species‐specific and likely depend on limpet body size. The histology of seminal receptacles revealed an uncommon disposition of spermatozoa (i.e., excessive debris and acrosomes detached from epithelium) in seminal receptacles of some parasitized limpets. However, this analysis failed to determine whether sperm derived from present matings or previous pea crab infection. Further studies are needed to address whether pea crabs interfere with the mating behavior of limpets and if the alterations in sperm disposition are a consequence of castration.
Anatomy and evolution of the head of Dorylus helvolus (Formicidae: Dorylinae): Patterns of sex‐ and caste‐limited traits in the sausagefly and the driver ant
Authors: Boudinot Brendon Elias , Moosdorf Olivia Tikuma Diana , Beutel Rolf Georg , Richter Adrian 
Published: August 2021

AbstractAnts are highly polyphenic Hymenoptera, with at least three distinct adult forms in the vast majority of species. Their sexual dimorphism, however, is overlooked to the point of being a nearly forgotten phenomenon. Using a multimodal approach, we interrogate the near total head microanatomy of the male of Dorylus helvolus, the “sausagefly,” and compare it with the conspecific or near‐conspecific female castes, the “driver ants.” We found that no specific features were shared uniquely between the workers and males to the exclusion of the queens, indicating independence of male and worker development; males and queens, however, uniquely shared several features. Certain previous generalizations about ant sexual dimorphism are confirmed, while we also discover discrete muscular presences and absences, for which reason we provide a coarse characterization of functional morphology. Based on the unexpected retention of a medial carinate line on the structurally simplified mandible of the male, we postulate a series of developmental processes to explain the patterning of ant mandibles. We invoke functional and anatomical principles to classify sensilla. Critically, we observe an inversion of the expected pattern of male‐queen mandible development: male Dorylus mandibles are extremely large while queen mandibles are poorly developed. To explain this, we posit that the reproductive‐limited mandible phenotype is canalized in Dorylus, thus partially decoupling the queen and worker castes. We discuss alternative hypotheses and provide further comparisons to understand mandibular evolution in army ants. Furthermore, we hypothesize that the expression of the falcate phenotype in the queen is coincidental, that is, a “spandrel,” and that the form of male mandibles is also generally coincidental across the ants. We conclude that the theory of ant development and evolution is incomplete without consideration of the male system, and we call for focused study of male anatomy and morphogenesis, and of trait limitation across all castes.
QCA based cost efficient coplanar 1 × 4 RAM design with set/reset ability
Authors: Naz Syed Farah , Ahmed Suhaib , Ko Seok‐Bum , Shah Ambika Prasad , Sharma Sparsh 
Published: August 2021

AbstractIn this paper, a loop based coplanar random access memory (RAM) cell with set/reset ability using quantum‐dot cellular automata (QCA) technology is first proposed. The operation of the RAM cell is validated physically as well as by simulations using QCADesigner tool. The energy dissipation analysis of the proposed RAM cell demonstrates that the proposed design dissipates very low energy. Additionally, the fault tolerance to single cell missing and addition defects of the proposed RAM cell is also presented. Further, we designed a coplanar 1 × 4 RAM which consists of four proposed RAM cells, one 2:4 decoder and one 5‐input majority voter along with the control signals required for the proper operation of the RAM. The proposed RAM cell and 1 × 4 RAM designs achieve performance improvement of up to 88.16% and 79.28%, respectively from the existing design in terms of QCA circuit cost. These structures can therefore be used to design efficient higher order RAM structures.
Changes in the ENSO–ISMR relationship in the historical and future projection periods based on coupled models
Authors: Varikoden Hamza , Hrudya Palakkal Puthan Veedu Haridas , Vishnu Rajagopalan Nair , Kuttippurath Jayanarayanan 
Published: August 2021

AbstractWe examine the projected changes in Indian summer monsoon rainfall (ISMR) and El Niño Southern Oscillation (ENSO) along with their changing relationships by the end of 21st century using Coupled Model Inter Comparison Project Phase5 (CMIP5) models. The historical (1951–2005) and future (2050–2099) projections of ISMR were assessed in addition to the changes in the ENSO–ISMR relationship during both the periods. The future period was selected as 2050–2099 in order to understand how the increase in greenhouse gas emissions and global warming by the end of 21st century will affect the ocean atmosphere interactions over the tropical Pacific Ocean and the associated rainfall characteristics over the Indian region. For the future projection, we considered Representative Concentration Pathways (RCP) 4.5 and 8.5 scenarios for the five models (CNRM‐CM5, GISS‐E2‐R, GISS‐E2‐R‐CC, HadGEM2‐CC and IPSL‐CM5A‐MR), which were selected from the 25 CMIP5 models based on their ability to capture the seasonal cycle and variability in the historical period. Although, all the five models simulate an increase in ISMR in both the RCPs, the increase is well simulated by CNRM‐CM5, HadGEM2‐CC and IPSL‐CM5A‐MR models mainly over the northeast, west coast, foothills of Himalayas, central India and the peninsular Indian regions. Substantial differences in simulating the ENSO–ISMR relationship are observed among the models in both historical and future RCP scenarios, and the observed relationship is well represented in GISS‐E2‐R‐CC, HadGEM2‐CC and IPSL‐CM5A‐MR models. The rainfall over most of the Indian regions shows a significant decrease (increase) during El Niño (La Niña) events in RCP 4.5. The changes in sea surface temperature (SST) anomalies and Walker circulations over the Indo‐Pacific region during El Niño and La Niña events are connected with the ISMR characteristics during these events in the RCP 4.5 scenario. On the other hand, the Indo‐Pacific region undergoes total warming (cooling) during El Niño (La Niña) events, and the Indo‐Pacific SST does not exhibit significant variability in the RCP 8.5 scenario. Hence, they are not well related with the observed ISMR patterns in that scenario.
Dissociation as a Transdiagnostic Indicator of Self‐Injurious Behavior and Suicide Attempts: A Focus on Posttraumatic Stress Disorder and Borderline Personality Disorder
Authors: Sommer Jordana L. , Blaney Caitlin , Mota Natalie , Bilevicius Elena , Beatie Brooke , Kilborn Kayla , Chang Unice , Sareen Jitender , El‐Gabalawy Renée 
Published: August 2021

AbstractDissociative symptoms and suicidality are transdiagnostic features of posttraumatic stress disorder (PTSD) and borderline personality disorder (BPD). The primary objective of this study was to examine associations between dissociation (i.e., depersonalization and derealization) and suicidality (i.e., self‐harm and suicide attempts) among individuals with PTSD and BPD. We analyzed data from the 2012–2013 National Epidemiologic Survey on Alcohol and Related Conditions (NESARC‐III; N = 36,309). The Alcohol Use Disorder and Associated Disabilities Interview Schedule for DSM‐5 was used to assess lifetime PTSD and BPD. Estimated rates of self‐harm among individuals who endorsed dissociation were 15.5%–26.2% for those with PTSD and 13.7%–23.5% for those with BPD, and estimates of suicide attempts among individuals who endorsed dissociation were 34.5%–38.1% for those with PTSD and 28.3%–33.1% for those with BPD. Multiple logistic regressions were conducted to examine the associations between dissociation (derealization, depersonalization, and both) and both self‐harm and suicide attempts among respondents with PTSD and BPD. The results indicated that dissociation was associated with self‐harm and suicide attempts, especially among individuals with BPD, aORs = 1.39–2.66; however, this association may be driven in part by a third variable, such as other symptoms of PTSD or BPD (e.g., mood disturbance, PTSD or BPD symptom severity). These results may inform risk assessments and targeted interventions for vulnerable individuals with PTSD, BPD, or both aimed at mitigating the risk of self‐harm and suicide.
Sharp difference in the rate of formation and stability of the Diels–Alder reaction adducts with 2,3‐dicyano‐1,4‐benzoquinone and N‐phenylimide‐1,4‐benzoquinone‐2,3‐dicarboxylic acid
Authors: Kiselev Vladimir D. , Kolesnikova Anastasia O. , Shulyatiev Alexey A. , Dinikaev Ildar F. , Kornilov Dmitry A. 
Published: August 2021

AbstractThis work reports new studies of the activity and Diels–Alder kinetics of a series of dienophiles: tetracyanoethylene (1), 2,3‐dicyano‐p‐benzoquinone (10), and N‐phenylimide‐1,4‐benzoquinone‐2,3‐dicarboxylic acid (11). Rate differences are interpreted in terms of the donor–acceptor properties of the reagents. The relative π‐acceptor properties of the dienophiles are probed by measuring their interaction energies with a series π‐donor solvents: benzene, toluene, o‐xylene, and chlorobenzene. The normalized interaction energies of 1, 10, and 11 are found to be 100:64:28. Despite the increased energy of the donor–acceptor interaction, dienophile 10 is 255 times less active in the reaction with 9,10‐dimethylanthracene than 11. It is suggested that this is due to the significantly lower energy of π‐bond cleavage in bicyclic dienophile 11, compared with monocyclic 10.
Online machine drawing pedagogy—A knowledge management perspective through maker education in theCOVID‐19 pandemic era
Authors: Sharma Gummaluri Venkata Surya Subrahmanya , Prasad Chilamkurti Lakshmi Venkata Ranga Sobhanachala Vara , Rambabu Velamala 
Published: August 2021

AbstractDrawing is a visual mode of communication. Teaching drawing requires one‐to‐one personal interaction among the tutor and the learner. The technical drawing is no exception, and it requires a considerable amount of imagination skills. On‐line mode of pedagogy shall be occupying a substantial portion of the mode of delivery in teaching and learning during, as well as, after the coronavirus disease 2019 (COVID‐19) pandemic era. This work focuses on the training and knowledge sharing of machine drawing skills through online mode, which is the requirement of the present era. A knowledge management perspective for machine drawing pedagogy is involved in this work. Challenges in the online pedagogy of machine drawing are deliberated through Ishikawa diagram and service Failure Modes and Effects Analysis. A maker education perspective of online machine drawing pedagogy is delineated. An approach toward knowledge workforce, knowledge transfer, and tacit knowledge is adopted for online teaching of machine drawing. Finally, conclusions are drawn in context of online pedagogy for a spatial visualization–based course like machine drawing.
Employees' citizenship behavior and workplace climate in promoting knowledge sharing
Authors: Park Sunyoung , Kim Eun‐Jee 
Published: August 2021

AbstractWe investigated the relationships among leadership, workplace climate, employee citizenship behavior (ECB), and knowledge sharing behavior. Survey data were collected from employees in the multi‐sector industry, including engineering and construction. We found that leadership affected workplace climate and citizenship behavior; workplace climate and citizenship behavior influenced knowledge sharing behavior; and workplace climate and citizenship behavior mediated between leadership and knowledge sharing. This study focused on how leadership, climate, and behavioral aspects affect individual behavior and the role of citizenship behavior and workplace climate.
Soybean yield and quality relative to Mollisols fertility with 7‐year consecutive cattle manure application under maize‐soybean rotation
Authors: Li Yansheng , Xie Zhihuang , Zhou Meng , Liu Changkai , Yu Zhenhua , Wu Junjiang , Jin Jian , Chen Yuan , Zhang Xingyi , Liu Xiaobing 
Published: August 2021

AbstractImproving soil productivity is the best way to increase grain production by 1% annually for feeding the rising population within the next 20 years in China. Nevertheless, how soybean yield and seed quality are affected by improving soil quality via consecutive manure addition are still unclear. A 7‐year consecutive cattle manure addition experiment was conducted under a soybean [Glycine max(Merrill.) L.] and maize (Zea maysL.) rotation in Mollisols. Treatments were as follows: CK: the non‐fertilizer control; CF: chemical fertilizer (NPK); CFM1: CF + 15 Mg ha−1cattle manure; CFM2: CF + 30 Mg ha−1cattle manure. The results showed CFM1 significantly increased soybean yield by 22%–105% and 21%–86% compared to CK and CF during 2014–2018. The consecutive cattle manure addition significantly increased the proportion of macro‐aggregates (1–0.25 mm) but decreased the proportion of micro‐aggregates (0.25–0.053 mm) and slit (<0.053 mm). The SOC storage in the large (>1 mm) and small macro‐aggregates (1–0.25 mm) was higher (by 24%–156%) under cattle manure addition than that under CK and CF. The soil productivity was enhanced by cattle manure addition because of the increase in soil available nutrients, that is, ‐N and ‐N, and Olsen‐P, all of which had positive correlations with the yield increase. At least 36% of soybean yield variation under CFM1 could be attributed to the indirect effect of pH value on increasing soil available nutrients concentrations. The cattle manure addition increased soybean seed oil concentration but decreased protein concentration. However, there were no differences in yield and quality between CFM1 and CFM2 after 7‐year of the treatment. It suggests that cattle manure addition at 15 Mg ha−1is a practical approach to improve Mollisols' productivity and SOC stability.