bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opening KATP Channels Induces Inflammatory Tolerance and Prevents Chronic Pain
Authors: Qian Cheng , Fan Yixin , Ji Lu-Lu , Wan Li , Jia Rumeng , Qin Xinmiao , Wang Yu , Wu Qi , Miao Chen , Hu Liang , Tao Xue-You , Liu Wen-tao 
Published: December 2021

Abstract
Background: Current treatments for chronic pain are not satisfactory, prompting a frantic search for new therapeutics and new therapeutic targets. Our previous study indicates KATP channel opener has analgesic effect, but the mechanism has not been elucidated. We speculated that KATP channel opener may increase suppressor of cytokine signaling (SOCS)-3 expression to induce inflammatory tolerance and attenuate chronic pain. Methods: The plantar incision (PI) surgery-induced postoperative pain was performed to establish chronic pain model. Growth arrest–specific 6 (Gas6)-/- and Axl-/- mice were used for signaling research. The microglia cell line BV-2 was cultured for in vitro experiments.Results: KATP channel opener significantly attenuated incision-induced mechanical allodynia in mice, associated with the up-regulated expression of SOCS3. Opening KATP channels induced the expression of SOCS3 dependent on Gas6/Axl signaling pathway in microglia. Opening KATP channels inhibits incision-induced mechanical allodynia by activating Gas6/Axl-SOCS3 signaling pathway. Opening KATP channels induces inflammatory tolerance to relieve neuroinflammation and postoperative pain.Conclusions: We demonstrated that KATP channel opening activated Gas6/Axl/SOCS3 signaling to induce inflammatory tolerance and relief chronic pain. We explored a new target for anti-inflammatory and analgesia by regulating the innate immune system, and provide a theoretical basis for clinical preemptive analgesia.
Identification of Pathogenesis and Prognoses of Relapse in Myeloma by Bioinformatic Method
Authors: Zhong Haoshu , Liu Yang , Duan Jialin , Chen Xiaomin , Xiong Hao , Liao Kunyu , Huang Chunlan 
Published: December 2021

Abstract
Background: Multiple myeloma (MM), the second most hematological malignancy, the molecular mechanism and pathogenesis of the relapse of MM is poorly understood. This study aimed to identify novel prognostic model for MM and explore potential mechanism of relapse. Methods: Gene expression data,clinical data(GSE24080) and HTseq-Counts files were downloaded from Gene Expression Omnibus (GEO) and TCGA database. Co-expression modules of genes were built by Weighted Correlation Network Analysis (WGCNA).KEGG and GO enrichment analysis were performed in each module. TATFs (tumor-associated transcription factors) were retrieved from the Cistrome. Twenty-two immune cell compositions was calculated by CIBERSORT algorithm.Univariate and multivariate Cox congression were performed and a predictive model by prognostic genes was constructed,the predictive power of the model was evaluated by Kaplan–Meier curve and time-dependent receiver operating characteristic (ROC) curves. Results: A total of 940 DEGs were identified,and in WGCNA analysis, yellow, brown and sky-blue modules were most associated with clinic traits.The yellow module related with the cell cycle and the brown and sky-blue modules correlated with cytokine and its receptors, where the M2 macrophage fraction is positively correlated with CCL18, CCL2, CCL8, CXCL12 and CCl23 were positively correlated with plasma cells by Cibersort analysis.Prognostic genes were identified and two genes (TPX2,PRAM1) were finally identified to construct a risk model for predicting EFS.
Prevalence of Typhoid fever and Helicobacter pylori infection in local population of Faisalabad, Pakistan
Authors: Ishaq Ali Raza 
Published: December 2021

Abstract
BackgroundTyphoid fever and Helicobacter pylori infection increases the secretion of gastric acid that leads to gastric carcinoma. In this research, we have tried to investigate the prevalence of typhoid and H. pylori in Faisalabad, Pakistan.MethodIn the present study, we collected the laboratory reports from different hospital of Faisalabad, Punjab, Pakistan. Statistical Package for Social Sciences (SPSS) (Chicago, IL), version 25 was used for data processing.ResultOur results demonstrated that H. pylori was most frequent in male at the age of 41–50-year-old and typhoid in female having 21-30 age.ConclusionConclusively, mostly female in typhoid and male in H. pylori were infected. People having 21-30 age were greatly infected by typhoid. The patients owing the 41-50 age were more susceptible for H. pylori infections. By comparing the co-infection rate, we found that typhoid is most common in over all age group than H. pylori.
Risk Factors For Readmission Among Patients Receiving Outpatient Parenteral Antimicrobial Therapy; A Retrospective Cohort Study
Authors: Douiyeb Sabrine , Court Jara R. de la , Tuinte Bram , Sombogaard Ferdi , Schade Rogier P. , Kuijvenhoven Marianne , Minderhoud Tanca , Sigaloff Kim C. 
Published: December 2021

Abstract
Background: In the Netherlands, home treatment with intravenous antimicrobial therapy is a relatively new concept. Although several studies have shown that outpatient parenteral antimicrobial therapy (OPAT) can be administered safely, people receiving antimicrobials at home remain at risk for adverse events, including readmission.Objectives: The aim of our retrospective study is to identify risk factors for readmission in patients discharged with OPAT.Method: Retrospective cohort study during a period of January 2016 - December 2018. Patients, age > 18 years, discharged with OPAT were included. Variables collected consisted of baseline demographics, complications, readmission within 30 days and treatment failure. Multivariate logistic regression analysis was performed to identify risk factors for readmission.Results: A total of 247 patients were included; the most common reason for OPAT was bone and joint infections (17%). Penicillin (37%), cephalosporin (26%) and vancomycin/aminoglycoside (15%) were the most commonly prescribed antimicrobials. Among the patients receiving medication subject to therapeutic drug monitoring (i.e. aminoglycosides or vancomycin), 51% (19/37) received weekly therapeutic drug monitoring. Receiving aminoglycosides or vancomycin (adjusted OR: 2.05; 95% CI, 1.30-3.25, p<0.05) and infection of prosthetic material (adjusted OR: 2.92, 95% CI, 1.11-7.65, p<0.05) were independent risk factors for readmission. Conclusion: Although patients receiving medication subject to therapeutic drug monitoring are at higher risk of readmission, only half of the patients discharged with aminoglycosides or vancomycin were monitored according to IDSA guidelines. A specialized team in charge of monitoring OPAT-patients is likely to increase the rate of monitoring to prevent readmissions and complications.
Genome-Wide Association Study Reveals the Genetic Architecture for Calcium Accumulation in Grains of Hexaploid Wheat (Triticum Aestivum L.)
Authors: Shi Xia , Zhou Zhengfu , Li Wenxu , Qin Maomao , Yang Pan , Hou Jinna , Huang Fangfang , Lei Zhensheng , Wu Zhengqing , Wang Jiansheng 
Published: December 2021

Abstract
Background: Hexaploid wheat (Triticum aestivum L.) is a leading cereal crop worldwide. Understanding the mechanism of calcium (Ca) accumulation in wheat is important to reduce the risk of human micronutrient deficiencies. However, the mechanisms of Ca accumulation in wheat grain are only partly understood. Results: Here, we performed a genome-wide association study to identify the genetic basis of Ca accumulation in wheat grain using an association population consisting of 207 varieties, with phenotypic data from three locations and the combined locations. In total, 18 non-redundant quantitative trait loci (QTLs) associated with Ca concentration were identified that explained, on average, 9.61%–26.93% of the phenotypic variation. Cultivars containing more superior alleles and fewer inferior alleles had increased grain Ca concentrations. Notably, six non-redundant loci were identified in at least two environments, indicating their stability across different environments. Searches of public databases revealed six putative candidate genes linked to Ca accumulation. Among them, two subunits of V-type Proton ATPase (TraesCS4A01G428900 and TraesCS3B01G241000) are encoded by genes associated with stable genetic loci on chromosomes 4A (AX-108912427) and 3B (AX-110922471), respectively, and they are typical generators of a proton gradient that might be involved in Ca homeostasis in wheat grain.Conclusion: This study could increases our understanding of the genetic architecture of grain Ca accumulation in wheat, and we plan to develop the identified superior alleles into molecular markers for wheat Ca biofortification pyramid breeding in the future.
Survey of Overwintering Trait in Chinese Rice Cultivars (Oryza Sativa L)
Authors: Liang Yongshu , Gong Junyi , Yan Yuxin , Wang Baobi , Gong Wenao , Wen Huan , Wu Qian , Nan Wenbin , Qin Xiaojian , Zhang Hanma 
Published: December 2021

Abstract
Overwintering (OW) rice can survive through the natural cold-winter field environment, exhibit a strong root system activity, sprout from rice tillering node in the following spring, and apparently reveal the cold resistance of rice during the whole growth stage. The successful utilization of cold-resistant rice is the most economical strategy for the cold-resistant rice cultivar breeding. This work aims to identify the OW rice for the future development of cold-resistant cultivars. Altogether 1034 Chinese rice cultivars were evaluated for their responses to low temperatures under the natural field cold-winter environment. The heading date (HD, d) and plant height (PH, cm) of 1034 rice cultivars ranged from 65 to 140 d in 2019, 65 to 150 d in 2020, ranged from 60 to 140 cm in 2019, 60 to 150 cm in 2020, and displayed slight difference between 2019 and 2020. Among them, altogether 262 (25.34%) Japonica rice cultivars could withstand cold to 4°C in December 2019 and distributed in 13 provinces of China, survive through the natural cold-winter field environment, and sprout from rice tillering node in March 2020. Only 24 (2.32%) japonica rice cultivars with resistance to 0°C in January 2021 distributed in seven provinces of China could also sprout from rice tillering node in March 2021. The present cold-resistant rice cultivars will provide beneficial breeding germplasm for future cold-resistant rice breeding and new strategies in elucidating the molecular mechanism of the cold resistance of rice.
Evaluation of the effects of pigments produced by environmental bacteria on cancer cells
Authors: Rezaei Aliakbar , bani Nazanin Hashemi , moazamian Elham 
Published: December 2021

Abstract
Cancers are a collection of incapacitating diseases in which cells initiate to divide and spread to adjacent tissues in an uncontrolled manner. Many researches demonstrated the potential of bacterial pigments as promising anticancer agents Therefore, in this study, the cytotoxic effects of bacterial pigments were evaluated in breast and colon cancer cell lines. In this study, a total of 90 samples were collected from air, water, and soil from 28 different geographical areas of Iran. Forty isolates were selected based on differences in colony color and geographic regions. The MTT assay was used to evaluate the effect of pigment on MCF-7 and SW-48 cells. Bacteria whose pigment had the highest cytotoxic effect on cell lines were selected. Accurate identification was performed using PCR, and their relative purity was measured using TLC. Bacteria isolated from any three ecosystems are capable of producing pigments. Pigment-producing bacteria are more abundant in the soil than air and water. Among pigment-producing bacteria, 3 isolates had the highest cytotoxicity on MCF-7 cells, and 3 isolates had the greatest effect on SW-48 cells. The results of sequencing of isolates at the BLAST site showed that 6 isolates with cytotoxic effects were identified (Micrococcus xinjiangensis, Dietzia, Arthrobacter agilis, Exigubacterium mexicanum, Bacillus beijingensis). Chromatography shows that these pigmented bacteria have different pigment components.Pigment extraction from bacteria can be used as a complementary therapy or other therapies for breast and colon cancer in the future.
Cumulative Triglyceride-glucose Index Is a Risk for CVD: A Prospective Cohort Study
Authors: Cui Haozhe , Liu Qian , Wu Yuntao , Cao Liying 
Published: December 2021

Abstract
Background Previous studies has shown a significant relationship between baseline triglyceride-glucose (TyG) index and cardiovascular disease (CVD). However, the long-term effect of TyG index and incident CVD remains uncertain. This study aimed to investigate the association between cumulative TyG index and the risk of CVD.Method In this study, we recruited individuals participating in Kailuan Study from 2006 to 2013 without stroke, myocardial infarction, and cancer in the four consecutive examinations. Cumulative TyG index was calculated by multiplying the average TyG index and the time between the two consecutive examinations. Cumulative TyG index levels were categorized into four quartile groups: Q1 group, ≤50.65 (as reference group), Q2 group, 50.65-53.86, Q3 group, 53.86-57.44, Q4 group, ＞57.44. The effect of Cumulative TyG index on CVD incidence was estimated by multivariable-adjusted Cox proportional hazard models.Result A total of 44,087 individuals participated in the final analysis. After a mean follow-up of 6.52±1.14 years, incident CVD, MI and stroke occurred in 2057, 395 and 1695, respectively. The multivariable-adjusted COX regression model showed the HR (95% CI) of CVD were Q2 1.25(1.08-1.44), Q3 1.22(1.05-1.40) and Q4 1.39(1.21-1.61), compared to Q1 group. Consistent results were obtained in the subgroup analyses and sensitivity analyses.Conclusion Cumulative TyG index was associated with increased risk of CVD. Maintaining a healthy level of TyG index within the desirable range and better control of cumulative TyG index is important for prevention of CVD.
Innate Immunity Stimulation During COVID-19 Pandemic: Challenge
Authors: palmieri beniamino , manenti antonio , VADALA' MARIA 
Published: December 2021

Abstract
AIM: We report an open spontaneous anecdotical retrospective survey of Corynebacterium parvum administration to 4000 fragile immune-depressed and multimorbid patients treated with a killed C. parvum strain to enhance the innate immunity integrating the adaptative immune response for a long standing antinfectious resistance. METHODS: 4000 patients (1900 men and 2100 women) with mild, moderate or chronic disease, appealing to our Second Opinion Medical Consultation Network, signed an informed consent and were injected subcutaneously with C.parvum. The treatment was followed up at 6 months after therapy, filling the short form of the medical outcome health survey questionnaire (SF-36), directly by the patients or its parents and monitoring the health regularly via telemedicine (Skype, WhatsApp, mail, etc) or physician’s visit.RESULTS: The main efficacy endpoints, as assessed by the SF-36 questionnaire, are significant improvements in the mental and physical role functioning score (p< 0.02), in general health, in social role functioning (p < 0.02), vitality (p < 0.03), and a significant reduction in bodily pain (p< 0.03). There was a quick (48-72 hours) symptoms improvement and/or complete regression of the herpetic eruptions observed in 1000 affected patients and of herpetic neuralgia (reduced in 80% of cases), also full recovery or frequency reduction (30%) of recurrent cystitis and prostatitis in 120 affected patients, and last, but not least, a life quality improvement in 100 oncologic patients of overall 200 cases. A significant increase in the lymphocyte count (p<0.01), mainly Helper and Killer lymphocytes, was noted after 6 months by Parvulan injection vs. the baseline.The asymptomatic SARS-CoV-2 patients, incidentally, enrolled in our survey, were tested at sixth months for antibodies against SARS-CoV-2 and 14 patients occurred high level of SARS-CoV-2 antibodies. The incubating Covid infections in the Parvulan injected patients even if rail and multi-morbid recovered in a short term (48-96 hours) and with benign clinical course, usually no need of further drugs administration except for the variants, which lasted on average one week and required some antipyretics, and low dose steroid for a few days.CONCLUSIONS: Our results confirm that C.parvum is quite safe and effective to support immune-compromised patients when epidemic or pandemic events rise the life risk and any kind of infections and complications rate.Further double-blind placebo evidence-based studies are urgently required, and our numerically substantial not sponsored spontaneous observation aims exclusively to promote further evidence based double blind institutional studies.
Field Experiments of Different Fracturing Designs in Tight Conglomerate Oil Reservoirs
Authors: Zhang Daiyan , Ma Shiying , Zhang Jing , Zhu Yue , Wang Bin , Zhu Jian , Fan Xibin , Liang Tianbo 
Published: December 2021

Abstract
Mahu oilfield is currently the largest tight conglomerate reservoir in the world, where Ma-131 and Ma-18 plays are the first two commercially developed reservoirs. In order to reduce the cost and explore the best fracturing parameters, field experiments have been conducted in these two plays since 2017. The types of proppant and fracturing fluid, the slickwater ratio, and the fracture spacing are mainly changed for comparison, and fracturing effects are evaluated to establish a reference for developing the neighboring plays in Mahu oilfield. This paper summarizes the fracturing parameters and production histories of 74 wells in Ma-131 and Ma-18 plays during four years of field operations. Firstly, results indicate that silica sands perform similar to ceramics in the Ma-131 play where the reservoir depth is smaller than 3300 m; however, in the Ma-18 play where the reservoir is deeper than 3500m, increasing the sand volume by 1.1 times still cannot reach the production in wells using ceramics. Secondly, when the fracture spacing is reduced, both oil production and water flowback become even smaller in wells using sands than those using ceramics; this is due to the increase of closure pressure and decrease of fluid volume per cluster respectively. Thirdly, when the crosslinked guar is replaced by the slickwater, no obvious change in oil production is noticed even though the volume of fracturing fluid is almost doubled; limited lengths of propped fractures due to the poor proppant-carrying ability of slickwater likely offset the production enhancement from the decrease of formation damage by slickwater. This paper summarizes learnings from the field experiments during four years of development in Mahu oilfield, and help guide the optimization of hydraulic fracturing parameters for future wells.
Spatial and temporal characteristics of soil erosion in a typical karst basin in southwest China and its response to the landscape pattern of rock desertification
Authors: Gao Jiayong , Li Rui , He Maolin , Yang Pingping , Jing Jun 
Published: December 2021

Abstract
Soil erosion is a process of migration and redistribution of soil substances in the landscape, which is regulated by topography, vegetation, human activities and their spatial pattern. At the watershed scale, changes in landscape pattern are important factors in determining the degree of soil erosion. Taking Dabang River Basin as the study area, based on the three phases of land use data, remote sensing image data and daily rainfall data from eight stations in the basin in 2010, 2015 and 2020, the rocky desertification factor (D) was introduced into the general soil loss equation RUSLE to calculate the soil erosion in Karst and non-Karst Areas in 2010, 2015 and 2020 respectively. The relationship between landscape pattern and soil erosion was analyzed from two aspects: type level index and landscape level index. The results showed that: 1) From 2010 to 2020, the average soil erosion modulus in The Dabang River Basin decreased first and then increased. The average soil erosion modulus in the non-karst region was about twice that in the karst region, and the average soil erosion modulus in the karst region decreased first and then increased. The mean soil erosion modulus in the non-karst area showed an increasing trend; 2) Under different slope grades, the erosion was mainly slight and mild, and the area of slight erosion was the largest, and the area of very strong and severe erosion increased as the slope increased. the area of strong, very strong and severe erosion increased in the slope zone below 15°, the area of light and moderate erosion decreased, and the area of slight, strong and very strong erosion increased in the slope zone from 15 to 25°, and the area of slight erosion increased in the slope zone above 25° area increased and light, moderate and strong erosion area decreased in the slope zone above 25°; 3) The landscape pattern of the Dadang River Basin changed significantly from 2010 to 2020. At the landscape level, the number of patches increased and the average patch area decreased. At the type level, the area of paddy field, woodland and shrubland decreases and the area of dry land, grassland, construction land and water body increased, and the dominant land type in the watershed changed from woodland to grassland; 4) The amount of soil erosion was positively correlated with patch type area, landscape percentage, maximum patch index and aggregation index, and positively correlated with edge density; 5) There was a linear relationship between soil erosion and Shannon diversity index (SHDI) and Shannon mean index (SHEI) at landscape level. The results can provide reference for land use planning and soil and water conservation measures.
Health Care System Decentralization Via Uberization, Concepts and Expected Efficiency Outcomes
Authors: Muradyan Armen , Aganyan Hakop , Manukyan Suren , Pilossyan Vagarshak , Kiraj Leon K. , Aganyan Inga , Aganyan Silvia , Avetisian Robert , Hovakimyan Ruben , Hakobyan Gagik 
Published: December 2021

Abstract
Background:According to statistical studies, about 3.6 million Americans miss medical appointments each year because of difficulties with transportation to a healthcare facility, and the impact of missed primary care appointments is estimated at billions of dollars annually. The access of the patient to necessary services is restricted and the role and functions of a medical doctor as responsible key decision maker is significantly diminished. Key responsibilities are still on the shoulders of the medical doctor, but decision-making power is shifted to middleman administrative bodies. This split between the responsibilities and decision-making bodies is destructive for the service of medicine. The aim of this study is to create a new management model in the health care system.Methods: To develop a new model of management in the health care system, we conducted a blind survey among 1,700 patients. To optimize the health care system, a decentralization health careservice method is proposed via uberization.Results: The method may continue with providing the request to a responding healthcare provider and receiving a response from the responding healthcare provider. The method may continue with establishing a bidirectional communication between the patient and the responding healthcare provider in real-time and receiving a plan of actions to treat the patient from the responding healthcare provider. The method may continue with receiving, from the diagnostic and laboratory service, the real-time vital parameters of the patient and making the real-time vital parameters available to the patient and the responding healthcare provider in an electronic medical record database.Conclusion: On the basis of our developed model of decentralization of the healthcare system via uberization, the implementation of the proposed model will increase the efficiency and availability of medical services.