bluuebunny/crossref_metadata_2025_split huggingface dataset:
Divergence among rice cultivars reveals roles for transposition and epimutation in ongoing evolution of genomic imprinting
Authors: Rodrigues Jessica A. , Hsieh Ping-Hung , Ruan Deling , Nishimura Toshiro , Sharma Manoj K. , Sharma Rita , Ye XinYi , Nguyen Nicholas D. , Nijjar Sukhranjan , Ronald Pamela C. , Fischer Robert L. , Zilberman Daniel 
Published: July 2021

Significance
Plant gene imprinting (allele-specific chromatin modification) results in parent-of-origin–dependent expression in endosperm, a nutritive tissue essential for embryo viability. Imprinted genes function in species reproductive isolation by causing embryo abortion in crosses. We analyzed the evolution of rice gene imprinting using four cultivars spanning domestication and the divergence of two subspecies 9,000 and 300,000 y ago, respectively. Most imprinted genes are imprinted across cultivars and enriched in regulatory functions. However, approximately 10% of imprinted genes have lost or gained imprinting, often associated with stably inherited epigenetic and genetic variation, suggesting a role in rice diversification. Our results highlight the role of transposable elements and epigenetic variation in shaping heritable changes in gene expression during rice evolution.
Beyond ‘fake news’?
Authors: Wright Scott 
Published: July 2021

Abstract
This article longitudinally analyses how Australian politicians engage with, and attack, journalists and the media more
generally on Twitter from 2011–2018. The article finds that attacks on journalists have increased significantly since 2016 when Trump came
to power, but this is largely the preserve of populist and far-right politicians. These politicians rarely call the media fake, instead
alleging bias or questioning the veracity or standards of reporting and production. Many politicians have a functional relationship with the
media, rarely criticising the media. Attacks are largely focused on the national public service broadcaster, the ABC, with limited attacks
on commercial media.
For family, for friends, for (true) love
Authors: Pak Vincent , Hiramoto Mie 
Published: July 2021

Abstract
We examine promotional materials produced by two organisations in Singapore, TrueLove.Is and Pink Dot, to investigate how
these two groups employ discourses of love to support their opposing views regarding the reconcilability of Christianity and same-sex desire.
TrueLove.Is is a Christian ministry that encourages LGB Christian Singaporeans to “come out, come home”, while Pink Dot is Singapore’s
largest and foremost LGBTQ movement. We identify similarities and differences in their persuasive discourse strategies regarding ideas of
love as discussed by lesbian Christian pastors. Although they position the idea of love similarly, their agendas are completely polarised.
TrueLove.Is takes the position that non-heteronormative activity is ungodly and sinful, while Pink Dot offers a reconciliation between Christianity
and same-sex desire. We employ Peterson’s (2016) approach to homophobic discourse analysis based on
Systemic Functional Linguistics and a comparative discourse analysis to investigate the ideologies that inform the two organisations’
materials about the treatment of LGBTQ Singaporeans.
A Small German Business Hotel on the Cutting Edge of Sustainability
Authors: Andre Julien , Duval Justine , Jeannon Marine , Yang Mo , Pi Marta Valls I 
Published: July 2021

Abstract
Creativhotel Luise, a business hotel of 93 rooms in the small town of Erlangen, Germany, operates on the principle that we are all responsible for the protection of the environment. The hotel puts this into practice through the concept of ‘creativity–sustainability’: creativity is used as a means of ensuring sustainability, whether in terms of economic viability, environmental responsibility, or social well-being.

Information
This case study is based on interviews with Ben Förtsch, owner & Managing Director of Creativhotel Luise. If a source is not provided for any data below, it will be from the information provided by Ben.
© CAB International 2021
Positioning public procurement as a procedural tool for innovation: an empirical study
Authors: Demircioglu Mehmet Akif , Vivona Roberto 
Published: July 2021

ABSTRACTProcurement has received scholarly attention as a valuable policy tool to reach desired outcomes in society, such as innovation. While interest has grown in analyzing the impact of the ‘substantive’ function of procurement (purchasing of goods and services), procurement is much more than purchases, and most public buyers’ activities are ‘procedural’, as they are aimed at improving the many internal stages of the procurement process. This study explains how procurement can be both a substantive and procedural tool, particularly in terms of innovation. Using the 2010 Innobarometer dataset that consist of 4,063 public organizations from 29 European countries, this study answers how public procurement, as a procedural policy tool, affects the implementation of public sector innovations. We find that procurement activities are positively related to innovation within public organizations. In particular, procurement as R&D for new technologies and services has an important and meaningful effect. We discuss implications for policy tools and public sector innovation literature, and we suggest that policy makers make use of more procedural tools such as procurement to increase innovation within public organizations.
Thermal axions with multi-eV masses are possible in low-reheating scenarios
Authors: Carenza Pierluca , Lattanzi Massimiliano , Mirizzi Alessandro , Forastieri Francesco 
Published: July 2021

Abstract
We revise cosmological mass bounds on hadronic axions in low-reheating cosmological scenarios, with a reheating temperature T
RH ≤ 100 MeV, in light of the latest cosmological observations. In this situation, the neutrino decoupling would be unaffected, while the thermal axion relic abundance is suppressed. Moreover, axions are colder in low-reheating temperature scenarios, so that bounds on their abundance are possibly loosened. As a consequence of these two facts, cosmological mass limits on axions are relaxed.
Using state-of-the-art cosmological data and characterizing axion-pion interactions at the leading order in chiral perturbation theory, we find in the standard case an axion mass bound m
a < 0.26 eV. However, axions with masses m
a ≃ 1 eV, or heavier, would be allowed for reheating temperatures T
RH ≲ 80 MeV. Multi-eV axions would be outside the mass sensitivity of current and planned solar axion helioscopes and would demand new experimental approaches to be detected.
Lost in Space? Relativistic Interstellar Navigation using an Astrometric Star Catalog
Authors: Bailer-Jones Coryn A. L. 
Published: July 2021

Abstract
The exploration of interstellar space will require autonomous navigation systems that do not rely on tracking from the Earth. Here I develop a method to determine the 3D position and 3D velocity of a spacecraft in deep space using a star catalog. As a spacecraft moves away from the Sun, the observed positions and velocities of the stars will change relative to those in a Earth-based catalog due to parallax and aberration. By measuring just the angular distances between pairs of stars, and comparing these to the catalog, we can infer the coordinates of the spacecraft via an iterative forward-modeling process. I perform simulations with existing star catalogs to demonstrate the method and to compute its performance. Using the 20 nearest stars and a modest angular distance measurement accuracy of 1″, the position and velocity of a spacecraft light years from the Sun moving at relativistic speeds can be determined to within 3

au

and 2

km

s

−
1

respectively. These accuracies improve linearly with the measurement accuracy, e.g., with angles measured to 1

mas

the navigation accuracy is 1000 times better. Performance can also be improved using more stars, or by including onboard measurements of the stars’ radial velocities, as these too are affected by the spacecraft’s position and motion.
An end-to-end heterogeneous graph attention network for Mycobacterium tuberculosis drug-resistance prediction
Authors: Yang Yang , Walker Timothy M , Kouchaki Samaneh , Wang Chenyang , Peto Timothy E A , Crook Derrick W ,   CRYPTIC Consortium, Clifton David A 
Published: July 2021

Abstract
Antimicrobial resistance (AMR) poses a threat to global public health. To mitigate the impacts of AMR, it is important to identify the molecular mechanisms of AMR and thereby determine optimal therapy as early as possible. Conventional machine learning-based drug-resistance analyses assume genetic variations to be homogeneous, thus not distinguishing between coding and intergenic sequences. In this study, we represent genetic data from Mycobacterium tuberculosis as a graph, and then adopt a deep graph learning method—heterogeneous graph attention network (‘HGAT–AMR’)—to predict anti-tuberculosis (TB) drug resistance. The HGAT–AMR model is able to accommodate incomplete phenotypic profiles, as well as provide ‘attention scores’ of genes and single nucleotide polymorphisms (SNPs) both at a population level and for individual samples. These scores encode the inputs, which the model is ‘paying attention to’ in making its drug resistance predictions. The results show that the proposed model generated the best area under the receiver operating characteristic (AUROC) for isoniazid and rifampicin (98.53 and 99.10%), the best sensitivity for three first-line drugs (94.91% for isoniazid, 96.60% for ethambutol and 90.63% for pyrazinamide), and maintained performance when the data were associated with incomplete phenotypes (i.e. for those isolates for which phenotypic data for some drugs were missing). We also demonstrate that the model successfully identifies genes and SNPs associated with drug resistance, mitigating the impact of resistance profile while considering particular drug resistance, which is consistent with domain knowledge.
Natriuretic peptide receptor B maintains heart rate and sinoatrial node function via cyclic GMP-mediated signalling
Authors: Dorey Tristan W , Mackasey Martin , Jansen Hailey J , McRae Megan D , Bohne Loryn J , Liu Yingjie , Belke Darrell D , Atkinson Logan , Rose Robert A 
Published: July 2021

Abstract

Aims
Heart rate (HR) is a critical indicator of cardiac performance that is determined by sinoatrial node (SAN) function and regulation. Natriuretic peptides, including C-type NP (CNP), have been shown to modulate ion channel function in the SAN when applied exogenously. CNP is the only NP that acts as a ligand for natriuretic peptide receptor-B (NPR-B). Despite these properties, the ability of CNP and NPR-B to regulate HR and intrinsic SAN automaticity in vivo, and the mechanisms by which it does so, are incompletely understood. Thus, the objective of this study was to determine the role of NPR-B signalling in regulating HR and SAN function.

Methods and results
We have used NPR-B deficient mice (NPR-B+/−) to study HR regulation and SAN function using telemetry in conscious mice, intracardiac electrophysiology in anaesthetized mice, high-resolution optical mapping in isolated SAN preparations, patch-clamping in isolated SAN myocytes, and molecular biology in isolated SAN tissue. These studies demonstrate that NPR-B+/− mice exhibit slow HR, increased corrected SAN recovery time, and slowed SAN conduction. Spontaneous AP firing frequency in isolated SAN myocytes was impaired in NPR-B+/− mice due to reductions in the hyperpolarization activated current (If) and L-type Ca2+ current (ICa,L). If and ICa,L were reduced due to lower cGMP levels and increased hydrolysis of cAMP by phosphodiesterase 3 (PDE3) in the SAN. Inhibiting PDE3 or restoring cGMP signalling via application of 8-Br-cGMP abolished the reductions in cAMP, AP firing, If, and ICa,L, and normalized SAN conduction, in the SAN in NPR-B+/− mice. NPR-B+/− mice did not exhibit changes in SAN fibrosis and showed no evidence of cardiac hypertrophy or changes in ventricular function.

Conclusions
NPR-B plays an essential physiological role in maintaining normal HR and SAN function by modulating ion channel function in SAN myocytes via a cGMP/PDE3/cAMP signalling mechanism.
Raphael’s ‘imperfect’ viol: a question of perspective
Authors: Hirsch Thilo , Haiduk Marina , Sayim Bilge 
Published: July 2021

Abstract
‘La viole parfaite’ is an article by Toon Moonen published in 1993, in which he describes his method of reconstructing an early viol on the basis of the instrument’s depiction in Raphael’s Saint Cecilia (c.1515–18). To date, the argument for using an iconographic source as a model for a Renaissance viol remains the same: all extant larger bowed stringed instruments dated to before the last quarter of the 16th century have been either so significantly modified that their original state is unrecognizable, or they are obvious counterfeits. This article examines Moonen’s reconstruction method in an interdisciplinary case study. Based on infrared reflectograms, for example, it was possible to show that the viol lying on the ground in Saint Cecilia was not subsequently altered. The reconstruction of the viol’s outline using 3d graphics yields completely new results, and perceptual psychology allows an analysis of the ‘reality effects’ possibly intended by the painter. In addition, this research also enables the examination of the general methodological requirements for reconstructing musical instruments from iconographic sources.
Long-term night shift work is associated with the risk of atrial fibrillation and coronary heart disease
Authors: Wang Ningjian , Sun Ying , Zhang Haojie , Wang Bin , Chen Chi , Wang Yuying , Chen Jie , Tan Xiao , Zhang Jihui , Xia Fangzhen , Qi Lu , Lu Yingli 
Published: July 2021

Abstract

Aims
The aim of this study was to test whether current and past night shift work was associated with incident atrial fibrillation (AF) and whether this association was modified by genetic vulnerability. Its associations with coronary heart disease (CHD), stroke, and heart failure (HF) were measured as a secondary aim.

Methods and results
This cohort study included 283 657 participants in paid employment or self-employed without AF and 276 009 participants free of CHD, stroke, and HF at baseline in the UK Biobank. Current and lifetime night shift work information was obtained. Cox proportional hazard models were used. Weighted genetic risk score for AF was calculated. During a median follow-up of 10.4 years, 5777 incident AF cases were documented. From ‘day workers’, ‘shift but never/rarely night shifts’, and ‘some night shifts’ to ‘usual/permanent night shifts’, there was a significant increasing trend in the risk of incident AF (P for trend 0.013). Usual or permanent night shifts were associated with the highest risk [hazard ratio (HR) 1.16, 95% confidence interval (CI) 1.02–1.32]. Considering a person’s lifetime work schedule and compared with shift workers never working nights, participants with a duration over 10 years and an average 3–8 nights/month frequency of night shift work exposure possessed higher AF risk (HR 1.18, 95% CI 0.99–1.40 and HR 1.22, 95% CI 1.02–1.45, respectively). These associations between current and lifetime night shifts and AF were not modified by genetic predisposition to AF. Usual/permanent current night shifts, ≥10 years and 3–8 nights/month of lifetime night shifts were significantly associated with a higher risk of incident CHD (HR 1.22, 95% CI 1.11–1.35, HR 1.37, 95% CI 1.20–1.58 and HR 1.35, 95% CI 1.18–1.55, respectively). These associations in stroke and HF were not significant.

Conclusion
Both current and lifetime night shift exposures were associated with increased AF risk, regardless of genetic AF risk. Night shift exposure also increased the risk of CHD but not stroke or HF. Whether decreasing night shift work frequency and duration might represent another avenue to improve heart health during working life and beyond warrants further study.
Prestack seismic inversion using a Rytov-WKBJ approximation
Authors: Huang Guangtan , Chen Xiaohong , Li Jingye , Luo Cong , Wang Hang , Chen Yangkang 
Published: July 2021

Summary
Approaches to seismic modeling using integral methods, notably Born-WKBJ (Wentzel-Kramers-Brillouin-Jeffreys), have seen an increase in applications in geophysical prospecting and near-surface exploration. Moreover, due to its linearity characteristic, which can fast and efficiently simulate full-waveform information, the Born-WKBJ based method performs well in seismic inversion. However, the Born approximation is the linearized wavefield simulation by Taylor expansion of the wavefield and omitting the high-order term, which cannot simulate the wavefiled accurately, including the amplitude, phase, and waveform information. For the seismic inversion, especially the amplitude variation with angle/offset (AVA/AVO) inversion, the amplitude is the most important element for estimating the parameters. In this paper, a Rytov-WKBJ approximation-based method, which can simulate the seismic amplitude information more accurately, is introduced to prestack seismic inversion. Besides, in order to improve the resolution of the inversion results, the ℓ1 − 2-norm regularized basis pursuit inversion (BPI) is introduced to the inversion algorithm. Then, we demonstrate the superiority of the proposed method with the zero-offset and angle-dependent seismic data simulation. The model tests show that the proposed method performs better than the conventional method significantly both on amplitude and phase of the seismic data. Finally, the inversion tests of synthetic and field seismic data indicate that the proposed method can obtain more accurate results with a higher resolution.
Germline ERBB3 mutation in familial non-small-cell lung carcinoma: expanding ErbB’s role in oncogenesis
Authors: McInerney-Leo Aideen M , Chew Hui Yi , Inglis Po-Ling , Leo Paul J , Joseph Shannon R , Cooper Caroline L , Okano Satomi , Hassall Tim , Anderson Lisa K , Bowman Rayleen V , Gattas Michael , Harris Jessica E , Marshall Mhairi S , Shaw Janet G , Wheeler Lawrie , Yang Ian A , Brown Matthew A , Fong Kwun M , Simpson Fiona , Duncan Emma L 
Published: July 2021

Abstract
Lung cancer is the commonest cause of cancer deaths worldwide. Although strongly associated with smoking, predisposition to lung cancer is also heritable, with multiple common risk variants identified. Rarely, dominantly inherited non-small-cell lung cancer (NSCLC) has been reported due to somatic mutations in EGFR/ErbB1 and ERBB2. Germline exome sequencing was performed in a multi-generation family with autosomal dominant NSCLC, including an affected child. Tumour samples were also sequenced. Full-length wild-type (wtErbB3) and mutant ERBB3 (mutErbB3) constructs were transfected into HeLa cells. Protein expression, stability, and subcellular localization were assessed, and cellular proliferation, pAkt/Akt and pERK levels determined. A novel germline variant in ERBB3 (c.1946 T &gt; G: p.Iso649Arg), coding for receptor tyrosine-protein kinase erbB-3 (ErbB3), was identified, with appropriate segregation. There was no loss-of-heterozygosity in tumour samples. Both wtErbB3 and mutErbB3 were stably expressed. MutErbB3-transfected cells demonstrated an increased ratio of the 80 kDa form (which enhances proliferation) compared with the full-length (180 kDa) form. MutErbB3 and wtErbB3 had similar punctate cytoplasmic localization pre- and post-epidermal growth factor stimulation; however, epidermal growth factor receptor (EGFR) levels decreased faster post-stimulation in mutErbB3-transfected cells, suggesting more rapid processing of the mutErbB3/EGFR heterodimer. Cellular proliferation was increased in mutErbB3-transfected cells compared with wtErbB3 transfection. MutErbB3-transfected cells also showed decreased pAkt/tAkt ratios and increased pERK/tERK 30 min post-stimulation compared with wtErbB3 transfection, demonstrating altered signalling pathway activation. Cumulatively, these results support this mutation as tumorogenic. This is the first reported family with a germline ERBB3 mutation causing heritable NSCLC, furthering understanding of the ErbB family pathway in oncogenesis.
Immortalized striatal precursor neurons from Huntington’s disease patient-derived iPS cells as a platform for target identification and screening for experimental therapeutics
Authors: Akimov Sergey S , Jiang Mali , Kedaigle Amanda J , Arbez Nicolas , Marque Leonard O , Eddings Chelsy R , Ranum Paul T , Whelan Emma , Tang Anthony , Wang Ronald , DeVine Lauren R , Talbot Conover C , Cole Robert N , Ratovitski Tamara , Davidson Beverly L , Fraenkel Ernest , Ross Christopher A 
Published: July 2021

Abstract
We have previously established induced pluripotent stem cell (iPSC) models of Huntington’s disease (HD), demonstrating CAG-repeat-expansion-dependent cell biological changes and toxicity. However, the current differentiation protocols are cumbersome and time consuming, making preparation of large quantities of cells for biochemical or screening assays difficult. Here, we report the generation of immortalized striatal precursor neurons (ISPNs) with normal (33) and expanded (180) CAG repeats from HD iPSCs, differentiated to a phenotype resembling medium spiny neurons (MSN), as a proof of principle for a more tractable patient-derived cell model. For immortalization, we used co-expression of the enzymatic component of telomerase hTERT and conditional expression of c-Myc. ISPNs can be propagated as stable adherent cell lines, and rapidly differentiated into highly homogeneous MSN-like cultures within 2 weeks, as demonstrated by immunocytochemical criteria. Differentiated ISPNs recapitulate major HD-related phenotypes of the parental iPSC model, including brain-derived neurotrophic factor (BDNF)-withdrawal-induced cell death that can be rescued by small molecules previously validated in the parental iPSC model. Proteome and RNA-seq analyses demonstrate separation of HD versus control samples by principal component analysis. We identified several networks, pathways, and upstream regulators, also found altered in HD iPSCs, other HD models, and HD patient samples. HD ISPN lines may be useful for studying HD-related cellular pathogenesis, and for use as a platform for HD target identification and screening experimental therapeutics. The described approach for generation of ISPNs from differentiated patient-derived iPSCs could be applied to a larger allelic series of HD cell lines, and to comparable modeling of other genetic disorders.