bluuebunny/crossref_metadata_2025_split huggingface dataset:
Free-living Turning Rather Than Gait Differentiates People with Chronic Mild Traumatic Brain Injury from Controls
Authors: Powell Dylan , Godfrey Alan , Parrington Lucy , Campbell Kody R. , King Laurie A. , Stuart Samuel 
Published: March 2021

Abstract
Background: Physical function remains a crucial component of mild traumatic brain injury (mTBI) assessment and recovery. Traditional approaches to assess mTBI lack sensitivity to subtle deficits post-injury, which can impact quality of life, daily function and can lead to chronic issues. Inertial measurement units (IMU) provide an objective alternative for measuring physical function of gait and turning and can be used in any environment. Our recent work has found that turning quality is more sensitive than the quantity of physical activity when comparing chronic mTBI and healthy controls. However, no studies have compared the quality of free-living gait and turning characteristics concurrently in chronic mTBI and healthy controls. This study aimed to determine whether free-living gait or turning is more sensitive in differentiating chronic mTBI from controls.Methods: Thirty-two people with chronic self-reported balance symptoms after mTBI (age: 40.88 ± 11.78 years, median days post injury: 440.68 days) and 23 healthy controls (age: 48.56 ± 22.56 years) were assessed for ~7 days using a single IMU at the waist on a belt. Free-living gait and turning characteristics were evaluated for chronic mTBI and controls using multi-variate analysis. Receiver operating characteristics (ROC) and Area Under the Curve (AUC) analysis were used to determine outcome sensitivity to chronic mTBI.Results: Free-living gait characteristics were not different in chronic mTBI and controls (all p>0.05). In contrast, all but two (number of turns and average velocity CV) free-living turning characteristics were significantly different between chronic mTBI and controls, whilst controlling for age and sex (Bonferroni adjusted p<0.002). The chronic mTBI group had larger turn angles and longer turn durations compared to controls. ROC and AUC analysis showed turn duration (AUC = 0.92) was the most sensitive measure for differentiating chronic mTBI from controls. Conclusions: Results show that turning rather than gait characteristics were significantly different between chronic mTBI and controls, with turn duration being the most sensitive measure. These results suggest turning is a suitable surrogate biomarker to assess and monitor chronic mTBI.
Intravital imaging of real-time endogenous actin dysregulation in proximal and distal tubules at the onset of severe ischemia-reperfusion injury
Authors: Corridon Peter R. , Karam Shurooq H. , Khraibi Ali A. , Khan Anousha A. , Alhashmi Mohamed A. 
Published: March 2021

Abstract
Severe renal ischemia-reperfusion injury (IRI) can lead to acute and chronic kidney dysfunction. Cytoskeletal modifications are among the main effects of this condition. The majority of studies that have contributed to the current understanding of IRI have relied on histological analyses using exogenous probes after the fact. Here we report the successful real-time visualization of actin cytoskeletal alterations in live proximal and distal tubules that arise at the onset of severe IRI. To achieve this, we induced fluorescent actin expression in these segments in rats with hydrodynamic gene delivery (HGD). Using intravital two-photon microscopy we then tracked and quantified endogenous actin dysregulation that occurred by subjecting these animals to 60 minutes of bilateral renal ischemia. Rapid (by 1-hour post-reperfusion) and significant (up to 50%) declines in actin content were observed. The decline in fluorescence within proximal tubules was significantly greater than that observed in distal tubules. Actin-based fluorescence was not recovered during the measurement period extending 24 hours post-reperfusion. Such injury decimated the renal architecture, in particular, actin brush borders, and hampered the reabsorptive and filtrative capacities of these tubular compartments. Thus, for the first time, we show that the combination of HGD and intravital microscopy can serve as an experimental tool to better understand how IRI modifies the cytoskeleton in vivo and provide an extension to current histopathological techniques.
Glaciochemistry and pigment producing ability of bacteria from roof of the world, the glaciers of Karakoram, Pakistan
Authors: Hassan Noor , Rafiq Muhammad , Haleem Abdul , Shah Aamir Ali , Hasan Fariha 
Published: March 2021

Abstract
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ~ 37% of its area glaciated. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan, in addition to the first information on the diversity of pigmented bacteria evaluated using culture-dependent techniques. Geochemical analyses revealed Ca2+ and SO42− to be the most abundant cation and anion species across all glacial samples, respectively. Total organic carbon (TOC), total nitrogen (TN) and total phosphorus (TP) were found in the sediments of all glaciers studied in current research. Bacterial species were capable of producing a variety of different pigments, including alloxanthin, astaxanthin, bacterioruberin, β-carotene, 19'-hexanoyloxyfucoxanthin, peridinin, violacein and zeaxanthin. Culturable bacterial diversity was studied using two molecular biomarkers, 16S rRNA and rpoB gene, with a total of 82 bacterial strains representing 25 genera identified across all glacial samples. This study provides the first characterization of glacier-associated, pigment-producing bacterial communities from the KMR. Findings are important for considerations of alternative sources of conventional pigment production in industrial fields.
Comparisons of neurotrophic effects of mesenchymal stem cells derived from adipose tissue, bone marrow, and cranial bone on chronic spinal cord injury
Authors: Otsuka Takashi , Maeda Yuyo , Kurose Tomoyuki , Nakagawa Kei , Mitsuhara Takafumi , Kawahara Yumi , Kurisu Kaoru , Yuge Louis 
Published: March 2021

Abstract
Background

Cell-based therapies with mesenchymal stem cells (MSCs) are considered as promising strategies for spinal cord injury (SCI). MSCs have unique characteristics due to difference in the derived tissues. However, relatively few studies have focused on differences in the therapeutic effects of MSCs derived from different tissues. Here, the therapeutic effects of adipose tissue-derived MSCs (aMSCs), bone marrow-derived MSCs (bMSCs), and cranial bone-derived MSCs (cMSCs) on chronic SCI model rats were compared.
Methods

MSCs were established from adipose tissue, bone marrow, and cranial bone collected. Neurotrophic factor expression of each MSC type was analyzed by real-time PCR. SCI rats were established with the weight-drop method and transplanted intravenously with MSCs at 4 weeks after SCI. Hind-limb motor function was evaluated from before injury to 4 weeks after transplantation. Endogenous neurotrophic factor and neural repair factor expression in spinal cord (SC) tissue were examined by real-time PCR and western blot analyses. Furthermore, the neurotrophic effects (i.e., neurite formation and elongation) of each MSC type were verified by co-culture with NG108-15 neural cells.
Results

Although there were no differences in the expression levels of cell surface markers and multipotency, expression of Bdnf, Ngf, and Sort1 (Nt-3) was relatively higher in cMSCs. Transplantation of cMSCs improved motor function of chronic SCI model rats. Although there was no difference in the degree of engraftment of transplanted cells in the injured SC tissue, transplantation of cMSCs enhanced Bdnf, TrkB, and Gap-43 mRNA expression and synaptophysin protein expression in injured SC tissue. In vitro, cMSCs co-cultured with NG108-15 cells promoted neurite formation and elongation.
Conclusion

As compared with MSCs derived other tissues, cMSCs highly express many neurotrophic factors which improved motor function in chronic SCI model rats by promoting endogenous neurotrophic and neural plasticity factors. These results suggest the efficacy of cMSCs in cell-based therapy for chronic SCI.
Effect of COVID-19 on Kawasaki Disease: Decrease Age of Onset and Increase Skin Manifestation
Authors: Esmaeilzadeh Hossein , Mortazavi Negar , Salehi Alireza , Fatemian Hossein , Dehghani Seyed Mohsen , Vardanjani Hossein Molavi 
Published: March 2021

Abstract
Kawasaki Disease (KD) is most common childhood vasculitis and cause of acquired heart disease for no apparent reason. There is some evidence indicating infectious agents as possible triggers for KD. During the COVID-19 pandemic, vasculitis has been a presentation of COVID-19 in children.ObjectiveWe performed this study to assess the association between KD and COVID-19.Methods and MaterialsWe evaluated KD hospitalized children during February to September 2020 for COVID-19 (group one) and compared their demographic, clinical, laboratory, and echocardiographic findings with KD patients from the same period time in 2019 (group two). We also compared the same data in COVID-19 positive and COVID-19 negative KD patients in 2020 pandemic period.ResultsSixty eight percent of group one KD patients were positive for COVID-19 during the pandemic period. KD Age of onset in the group one was lower than group two (4.38 years VS 5.5 years, P-value= 0.044). There was no difference in the demographic, clinical, laboratory, and echocardiographic features of the patients during and before the COVID-19 pandemic (p-value > 0.05). Moreover, Comparing COVID-19 positive and negative patients, the incidence of rash was higher within COVID-19 positive cases (p<0.05), and coronary artery abnormalities were more prevalent in COVID-19 negative cases (p < 0.05).ConclusionAdmission rate of KD does not have significant difference during the COVID-19 pandemic but 68% of KD admitted patient were COVID-19 positive. Age of onset for KD during the COVID-19 pandemic was lower and skin manifestation was higher than the same period time in last year.
Simultaneous Supraclavicular Lymph Node Transfer and Lymphovenous Anastomosis for the Surgical Treatment of End-Stage Lymphedema
Authors: Moon Kyung-Chul , Yoon In-Jae 
Published: March 2021

Abstract
BackgroundVarious surgical options are available to treat lymphedema, such as direct excisional debulking surgery, suction-assisted lipectomy debulking, lymphovenous anastomosis (LVA), and vascularized lymph node transplantation (VLNT). However, no studies have addressed simultaneous surgery with both LVA and VLNT for patients with advanced-stage lymphedema. Case Presentation A 72-year-old female with bilateral lower extremity lymphedema refractory to nonsurgical management was admitted to our lymphedema clinic. This patient had a history of lymphoma and treated with radiotherapy on right inguinal area 26 years ago. Interestingly, the patient developed lymphedema on both the right and left lower extremities although she had radiotherapy on her right inguinal area. The patient underwent simultaneous vascularized lymph node transfer and lymphovenous anastomosis for treatment of end-stage lymphedema. Significant reduction in circumference and volume of lower extremity was achieved following simultaneous vascularized lymph node transfer and lymphonvenous anastomosis Conclusion The authors recommend simultaneous VLNT and LVA surgeries as the first treatment option for patients with end-stage lymphedema.
The Use of Ondansetron for the Treatment of Nausea in Dogs With Vestibular Syndrome
Authors: Foth S. , Meller S. , Kenward H. , Elliot J. , Pelligand L. , Volk H. A. 
Published: March 2021

Abstract
Background: Vestibular syndrome is often accompanied by nausea. Drugs currently approved for its treatment have been developed to stop vomiting but not nausea. The efficacy of 5‑HT3 receptor antagonists to reduce nausea has been described for chemotherapy, but not for nausea secondary to vestibular disorders.Methods: Sixteen dogs with vestibular syndrome-associated nausea were included in the open-label, multicentric study. The intensity of nausea-like behaviour was analysed before ondansetron administration (0.5 mg/kg i.v.) and 2 h afterwards, using a validated 5-point-scale. The occurrence and frequency of salivation, lip licking, restlessness, vocalisation, lethargy, and vomiting were assessed.Results: All dogs initially showed signs of nausea, whereas only 31% showed vomitus. The intensity of nausea was significantly reduced in all dogs (p≤0.0001) 2 h after ondansetron administration, including the clinical signs of nausea analysed in 11 dogs (salivation [p=0.0078], lip licking [p=0.0078], restlessness [p=0.0039], and lethargy [p=0.0078]) except for vocalisation (p>0.9999).Conclusion: The results provide preliminary evidence of the potential benefit of ondansetron in the treatment of nausea, which was present in all examined dogs. Vomiting was only observed in 5 dogs indicating that nausea can occur separately and should not be perceived only as a preceding stimulation of the vomiting centre.
Novel homozygous mutations in Pakistani families with recessive Charcot-Marie-Tooth disease
Authors: Kanwal Sumaira , Choi Yu Jin , Lim Si On , Choi Hee Ji , Park Jin Hee , Nuzhat Rana , khan Aneela , Perveen Shazia , Choi Byung-Ok , Chung Ki Wha 
Published: March 2021

Abstract
Background

Charcot-Marie-Tooth disease (CMT) is a group of genetically and clinically heterogeneous peripheral nervous disorders. Few studies have identified genetic causes in the Pakistani CMT patients.
Methods

This study was performed to identify pathogenic mutations in five consanguineous Pakistani CMT families negative for PMP22 duplication. Genomic screening was performed by application of whole exome sequencing
Results

We identified five pathogenic or likely pathogenic homozygous mutations in four genes: c.2599C > T (p.Gln867*) and c.3650G > A (p.Gly1217Asp) in SH3TC2, c.19C > T (p.Arg7*) in HK1, c.247delG (p.Gly83Alafs*44) in REEP1, and c.334G > A (p.Val112Met) in MFN2. All the mutations were not reported in the CMT patients. Mutations in the SH3TC2, HK1, REEP1, and MFN2 have been reported to be implicated to CMT4C, CMT4G, dHMN5B (DSMA5B), and CMT2A, respectively. The genotype-phenotype correlations were confirmed in all the examined families. We also confirmed that both alleles from the homozygous variants were originated from a single founder using homozygosity mapping.
Conclusions

This study found five novel mutations as the underlying causes of CMT. Pathogenic mutations in SH3TC2, HK1, and REEP1 have been reported rarely in other populations, suggesting ethnic-specific distribution. This study will be useful for the exact molecular diagnosis and treatment in the Pakistani CMT patients.
Carvacrol attenuates amikacin-induced nephrotoxicity in the rat
Authors: Dost Atta Mohammad , Gunata Mehmet , Ozhan Onural , Yildiz Azibe , Vardi Nigar , Tunc Selahattin , Cigremis Yilmaz , Duman Ahmet Sefa , Colak Cemil , Parlakpinar Hakan 
Published: March 2021

Abstract
Amikacin (AK) is frequently used in the treatment of gram-negative and some gram-positive infections. However, its use is limited due to nephrotoxicity due to the increase in reactive oxygen radicals. The aim of this study was to investigate the role of carvacrol (CAR) against AK-induced nephrotoxicity in rats. Thirty-two Sprague Dawley rats were randomly divided into four groups as control (Vehicle), AK (400 mg/kg), CAR + AK (80 mg/kg CAR + 400 mg/kg AK), and AK + CAR (400 mg/kg AK + 80 mg/kg CAR) groups. AK and CAR were administered via intramuscular and per-oral for 7 days, respectively. Blood and kidney tissue samples were taken at the end of the experiment. Renal function and histopathological changes were compared, and the relevant parameters of oxidative stress and inflammation were detected. Histopathological findings (necrotic changes and dilatation and inflammatory cell infiltration) significantly increased in the AK group compared to the control group. Also, the rats in the AK group lost weight significantly. It was found that CAR treatment before and after AK significantly improved nephrotoxicity histopathologically (p < 0.05). However, this improvement was not detected biochemically. These results show that CAR treatment before and after AK improves nephrotoxicity in the histopathological level.
Empower Saving Energy into Smart Communities using Social Products with a Gamification Structure for Tailored Human-Machine Interfaces within Smart Homes
Authors: Méndez Juana Isabel , Ponce Pedro , Meier Alan , Peffer Therese , Mata Omar , Molina Arturo 
Published: March 2021

Abstract
Residential buildings can contribute to save energy and to decrement electricity consumption in the world. On the other hand, the Internet of Things has allowed the implementation of smart homes that can profile the users. Nevertheless, end-users are not accepting the smart homes due to behavioral problems and usability problems with the Human-Machine Interface (HMI) or with the household appliances. As a solution, social products promote the interaction between the smart home and the consumer by including gamification features in the interface. Thus, smart homes can interact and compete with other houses to reduce energy consumption. Therefore, this paper proposes a three-step framework that takes advantage of social products to promote interaction between smart homes within a smart community to reduce electrical energy consumption. The first step collects from the literature review, the characteristics of the end-users, the behavioral and usability problems, and the most common gamification elements that teach, engage and motivate the user to reduce energy consumption. The second step proposes the gamification elements required for a tailored HMI in each social product and smart home through a fuzzy logic decision. The third step evaluates the interaction between social products in smart homes and the users to test which smart home is reducing more energy consumption. Finally, a three-level tailored gamified mock-up is depicted: level 1 for a single social product, level 2 for the smart home, and level three for the smart community. This mock-up can be implemented in small communities as residential complexes.
Diagnosis and Treatment of Secretory Carcinoma of the Oral Cavity: Report of Two Cases and Pooled Analysis of the Recent Literature
Authors: Ogawa Masaru , Yokoo Satoshi , Yamaguchi Takahiro , Suzuki Keisuke , Seki Mai , Shimizu Takahiro , Kurihara Jun , Makiguchi Takaya 
Published: March 2021

Abstract
Background: Secretory carcinoma (SC) is a malignancy of the salivary glands, which is similar to SC of the breast in terms of its association with neurotrophic tyrosine receptor kinase (NTRK) fusion-positive gene. SC is a recently described salivary gland tumor, and there are few reports describing oral minor salivary gland-derived SC. We reported two cases of SC in the oral cavity and reviewed the literature.Case presentation: The patients included a 65-year-old Japanese woman presented with a mass of the upper lip and an 84-year-old Japanese man presented with a mass on the buccal mucosa, respectively. Diagnosis was based on histomorphological and immunohistochemical findings and identification of a specific translocation of the ETS variant 6-neurotrophic receptor tyrosine kinase 3 (ETV6-NTRK3) gene fusion. Case 1 was finally diagnosed using reverse transcription-polymerase chain reaction using formalin-fixed paraffin-embedded tissue samples, while case 2 was diagnosed using fluorescence in situ hybridization analysis. Conclusion: It is highly likely for many cases of SC to be initially diagnosed as acinic cell carcinoma (AciCC) owing to their similar histological findings. The treatment strategy for minor salivary gland-originated SC is the same as that for AciCC, but SC is often highly malignant and involves a high risk of cervical lymph node metastasis. Making an accurate diagnosis in cooperation with pathologists and confirming the presence of the ETV6-NTRK3 fusion gene using genetic analysis is important.
SGB Postconditioning Improves Intestinal Barrier Function by Inhibiting Autophagy in Conscious Rats Following Hemorrhagic Shock and Resuscitation
Authors: Li Zhong-Hua , Wang Wen-Di , Sun Qi , Suo Ting-Jiao , Zhai Jia-Yi , Du Hui-Bo , Zhao Zhen-Ao , Li Fu-Long , Niu Chun-Yu , Zhao Zigang 
Published: March 2021

Abstract
Background

Intestinal barrier dysfunction is the critical link of distant organ injury caused by hemorrhagic shock. The role of autophagy in ischemic intestinal injury has been paid more and more attention. Prophylactic treatment of stellate ganglion block (SGB) reduces intestinal barrier dysfunction induced by haemorrhagic shock. This study investigated the role of SGB post-processing on improved bowel barrier function and autophagy-related mechanisms of action after haemorrhagic shock.
Methods

The model of hemorrhagic shock in conscious rats was established, and the rats were treated with SGB, autophagy inhibitor 3- methyladenine (3-MA) at the time of fluid resuscitation. We detected the survival rate, intestinal loop blood flow, intestinal barrier permeability, intestinal morphology, wet/dry ratio (W/D), the intestinal barrier and autophagy marker proteins. Simultaneously, we also observed the effect of autophagy activator rapamycin (RAPA) on SGB.
Results

SGB postconditioning significantly prolonged the overall survival and enhanced survival rate of 72 hours in rats after hemorrhagic shock. SGB post-processing and 3-MA administration improved the intestinal morphology and intestinal permeability, increased the intestinal loop blood flow and expression of ZO1, Occludin and Claudin-1, and decreased the intestinal W/D and LC3 and Beclin-1 expressions, expected for increased P62. Meanwhile, RAPA partially inhibited the effect of SGB on above indices in haemorrhagic shock rats.
Conclusion

SGB postconditioning alleviates the intestinal barrier dysfunction caused by haemorrhagic shock, which is related to the inhibition of excessive autophagy.
Coherence transfer in two-pulse double quantum (DQ) and five-pulse double- quantum modulation (DQM) sequences in EPR: Orientation selectivity, structural sensitivity and distance measurements
Authors: Misra Sushil K. , Salahi Hamid Reza 
Published: March 2021

Abstract
Double-quantum (DQ) coherence transfers in two-pulse DQ and five-pulse DQM (double quantum modulation) EPR pulse sequences, utilized for orientation selectivity and distance measurements in biological systems using nitroxide biradicals, are investigated. Analytical expressions, along with numerical algorithms, for EPR signals are given in full details. Please see manuscript .pdf for full abstract.
Branched 2-amino-1,3-dicyanocyclopenta-1,3-diene
Authors: Ros Francisco 
Published: March 2021

Abstract
Reaction of 2-chloroisobutyrophenonewith two equivalents of malononitrile anion furnishes 2-amino-1,3-dicyano-5,5-dimethyl-4-phenylcyclopenta-1,3-diene. The cyclic compound represents the novel 2-amino-1,3-dicyanocyclopentadiene structure. The unique 1-cyano-2-amino-3-cyano arrangement in the cyclopentadiene brings about a strong polarization of the electronic configuration of the diene system that is conformed by two opposite dipolar halves. The polarized electronic configuration accounts for the extreme persistence manifested by the cyclopentadiene. The compound owns a vivid lemon-hued yellow color consequent to an unusually intense n ®p*absorption of a cyano group in the extensively conjugated compound. This is built up by consecutive one-pot reaction of two molecules of malononitrile carbanion and the ketonic substrate followed by a new tandem carbon-carbon cyclization with final elimination of cyanate ion.