bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression profiling analysis of autophagy-related genes in cervical cancer
Authors: Huang Zhiyuan , Wang He , Liu Min , Li Xinrui , Zhu Lei , Dong Lin , Wang Guangxue , Li Fang , Li Qinchuan 
Published: June 2021

Abstract
Background: It has been demonstrated by studies globally that autophagy took part in the development of cervical cancer (CC). Few studies concentrated on the correlation between overall survival and CC patients. We retrieved significant autophagy-related genes (ARGs) correlated to the process of cervical cancer. They may be used as prognosis marker or treatment target for clinical application.Methods: Expressions level of genes in cervical cancer and normal tissue samples were obtained from GTEx and TCGA database. Autophagy-related genes (ARGs) were retrieved accroding to the gene list from HaDB. Differentially expressed autophagy related genes (DE-ARGs) related to cervical cancer were identified by Wilcoxon signed-rank test. ClusterProfiler package worked in R software was used to perform GO and KEGG enrichment analyses. Univariate propotional hazard cox regression and multivariate propotional hazard cox regressions were applied to identify DE-ARGs equipped with prognostic value and other clinical independent risk factors. ROC curve was drawn for comparing the survival predict feasibility of risk score with other risk factors in CC patients. Nomogram was drawn to exhibit the prediction model constructed accroding to multivariate cox regression. Correlations between Differentially expressed autophagy related genes (DE-ARGs) and other clinical features were investigated by t test or Cruskal wallis analysis. Correlation between Immune and autophagy in cervical cancer was investigated by ssGSEA and TIMER database. Results: Fifty-six differentially expressed ARGs (DE-ARGs) were retrieved from cervical cancer tissue and normal tissue samples. GO enrichment analysis showed that these ARGs involved in autophagy, ubiquitination of protein and apoptosis. Cox regression medel showed that there were six ARGs significantly associated with overall survival of cervical caner patients. VAMP7 (HR = 0.599, P= 0.033) and TP73 (HR = 0.671, P= 0.014) played protective roles in survival among these six genes. Stage (Stage IV vs Stage I HR = 3.985, P<0.001) and risk score (HR = 1.353, P< 0.001) were sorted as independent prognostic risk factors based on multivariate cox regression. ROC curve validated that risk score was preferable to predict survival of CC patients than other risk factors. Additionally, we found some of these six predictor ARGs were correlated significantly in statistic with tumor grade or stage, clinical T stage, clinical N stage, pathology or risk score (all P< 0.05). The immune cells and immune functions showed a lower activity in high risk group than low risk group which is distincted by median risk score. Conclusion: Our discovery showed that autophagy genes involved in the progress of cervical cancer. Many autophagy-related genes could probably serve as prognostic biomarkers and accelerate the discovery of treatment targets for CC patients.
Association of HLA and Mutated CCR5 With the Clinical Course of the Disease in Subjects With mild / moderate disease following COVID-19 infection
Authors: Fischer Johannes C , Schmidt Albrecht G , Boelke Edwin , Uhrberg Markus , Keitel Verena , Feldt Torsten , Jensen Björn , Häussinger Dieter , Adams Ortwin , Schneider E. Marion , Balz Vera , Enczmann Jürgen , Rox Jutta , Hermsen Derik , Schulze-Bosse Karin , Kindgen-Milles Detlef , Knoefel Wolfram Trudo , Griensven Martijn van , Haussmann Jan , Tamaskovics Balint , Plettenberg Christian , Scheckenbach Kathrin , Corradini Stefanie , Pedoto Alessia , Maas Kitti , Schmidt Livia , Grebe Olaf , Esposito Irene , Erhardt Anja , Peiper Matthias , Buhren Bettina Alexandra , Calles Christian , Stöhr Andreas , Lichtenberg Artur , Freise Noemi , Lutterbeck Matthias , Rezazadeh Amir , Budach Wilfried , Matuschek Christiane 
Published: June 2021

Abstract
COVID-19, the pandemic infection caused by SARS-CoV-2, may take highly variable clinical courses, ranging from symptom-free and pauci-symptomatic to fatal disease. The goal of the current study was to assess the association of COVID-19 clinical courses controlled by patients’ adaptive immune responses without progression to severe disease with patients’ Human Leukocyte Antigen (HLA) genetics, the presence or absence of near-loss-of-function delta 32 deletion mutant of C-C chemokine receptor type 5 (CCR5) and AB0 blood group antigens. We further analyzed the association of these immunogenetic background characteristics with patients’ humoral antiviral immune response patterns, assessed longitudinally. The study enrolled 157 convalescent adult patients followed up for up to 250 days. Univariate HLA analyses identified putatively protective HLA alleles (HLA class II DRB1*01:01 and HLA class I B*35:01, with a trend for DRB1*03:01) associated with reduced durations of disease and decreased (rather than increased) total anti-S IgG levels providing virus neutralizing capacity comparable to non-carriers. Conversely, analyses also identified HLA alleles (HLA class II DQB1*03:2 und HLA class I B*15:01) not associated with such benefit in the patient cohort of this study. Hierarchical testing by Cox regression analyses confirmed the significance of the protective effect of the HLA alleles identified (when assessed in composite) in terms of disease duration, whereas AB0 blood group antigen heterozygosity was found to be significantly associated with disease severity (rather than duration) in our cohort. A seeming association of a heterozygous CCR5 delta 32 mutation with prolonged disease duration suggested by univariate analyses was not confirmed by hierarchical multivariate testing.In conclusion, the current study shows that the presence of certain "protective" HLA alleles is of even stronger association with reduced duration of mild and moderate COVID-19 than age or any other potential risk factor assessed. Prospective studies in larger patient populations assessing the impact of HLA genetics on the capacity of mounting protective vaccination responses may be warranted.
Dexmedetomidine Protects against Hepatic Ischemia-Reperfusion Injury by inhibiting Endoplasmic Reticulum Stress and Cell Apoptosis in Rats
Authors: Li Hang , Tang Jilang , Zhang Weiqi , Ai Liping , Zhang Shixia 
Published: June 2021

Abstract
Background: Hepatic ischemia-reperfusion injury (IRI) remains a major complication of liver surgery, dexmedetomidine (DEX) has a certain protective effect on liver during ischemia-reperfusion, but the underlying mechanisms are not fully understood. This study explored the protective effects of DEX and investigated whether DEX protects against hepatic IRI by inhibiting endoplasmic reticulum stress (ERS) and its downstream apoptotic pathway in a rat model. Methods: Thirty-six male Sprague-Dawley (SD) rats were divided into six groups: S, IR, DL, DM1, DH and DM2 group. Group S was subjected to laparotomy, and exposure of the portal triad without occlusion. I-R injury model was induced by clamping the portal vessels supplying the middle and left hepatic lobes for 30 min in IR, DL, DM1, DH and DM2 group. Then DL, DM1, DH group received DEX of 25 μg/kg, 50 μg/kg and 100 μg/kg intraperitoneally at 30 min before ischemia, respectively, DM2 group received 50 μg/kg DEX intraperitoneally 30 min after reperfusion, and IR group received normal saline. After 6 h of reperfusion, assessment of liver function, histopathology, oxidative stress was performed. The liver cell microstructure was detected by transmission electron microscopy. Hepatocyte apoptosis was determined by TUNEL assay. Real-time PCR, Western blotting were performed to analyze various ERS molecules. Results: We observed that DEX protected the liver by alleviating hepatocytes damage, reducing the content of ALT and MDA, increasing the activity of SOD, reducing the number of TUNEL-positive cells, down-regulating the expression of GRP-78, PERK, ATF-6, Caspase-12 mRNA, and p-PERK, p-IRE-1 α, CHOP proteins, up-regulating Bcl-2 protein. The effect of 50 μg/kg DEX is superior to 25 μg/kg DEX, but not significantly different from 100μg/kg DEX. There was no significant difference in the above monitoring indexes between DM1 and DM2 group. Conclusions: DEX protects the liver from IRI by inhibiting ERS and cell apoptosis. The protective effect of DEX was dose-dependent in a certain dose range, both DEX administered prior to ischemia and following reperfusion markedly reduced liver injury induced by hepatic IRI in mice.
Early Serum HBV RNA Level in Combination With the HBeAg Response Can Effectively Predict the HBeAg Response in Patients on Nucleos(t)ide Analogue Therapy (ClinicalTrials.gov (NCT03909191)
Authors: LIU YAYUN , LIAO WEI , XU AIJING , YIN WEI , YU JIAO , FAN WENHAN , XUE JIANYA , LIANG XUESONG 
Published: June 2021

Abstract
Background: Serum HBV RNA level has the potential to monitor antiviral therapy in patients with chronic hepatitis B. This study aimed to explore serum HBV RNA dynamic change pattern and its predict value on the efficacy of 96 weeks nucleos(t)ide analogue (NA) treatment in HBeAg-positive and HBeAg-negative patients with chronic hepatitis B (CHB).Methods: A real-life cohort study of 78 patients with CHB on NA treatment was conducted. Dynamic change patterns of serum HBV RNA and correlation with other HBV markers in the early treatment period of 96 weeks of NA treatment in patients with CHB were determined and compared. The performance of serum HBV RNA on treatment efficacy was analyzed by receiver operating characteristic (ROC) analyses. Results: HBeAg-positive and HBeAg-negative patients with CHB had similar viral change patterns during NA treatment. Serum HBV RNA level was consistently correlated with HBeAg and HBsAg titers in HBeAg-positive patients during NA treatment, but serum HBV RNA was only moderately correlated with serum HBV DNA level in HbeAg-negative patients before treatment. Serum HBV RNA decreased more rapidly in patients with the HBeAg seroconversion (SC) response than in patients without the HBeAg SC response; it had good early discriminatory ability for the HBeAg response with area under the ROC curve (AUROC) of 0.70 and 0.730 at 12 and 24 weeks of treatment, respectively. The cutoff value of serum HBV RNA of 4.31 log cps/mL in combination with the HBeAg decrease degree of 1.55 at 24 weeks of treatment had a good two-way predictive capability for the HBeAg response (PPV%: 83.33% and NPV%: 81.25%), and the specificity was 96.30%. Conclusion: Serum HBV RNA level had early discriminatory ability for the HBeAg response. Early HBeAg response can improve the discriminatory ability of serum HBV RNA.
Dandelion Root Extract Affects the Proliferation, Survival and Migration of Cervical Cancer Cell Lines
Authors: VENEZUELA Raúl Fernando , MOSMANN Jessica Paola , MUGAS María Laura , KIGUEN Ana Ximena , MONTOYA Susana Carolina NUÑEZ , KONIGHEIM Brenda Salome , CUFFINI Cecilia Gabriela 
Published: June 2021

Abstract
Background

Taraxacum officinale has been shown to exert diverse biological activities, in someones cancer cell lines. Also in traditional medicine have been its used fresh latex to treat warts (non-malignant HPV infection); however, its effect over cervical cancer cell lines is yet to be explored. The aim was to evaluate the effects of a root extract of Taraxacum officinale on the proliferation, survival and migration of cervical cancer cell lines.
Methods

Ethanolic extract was obtained from T. officinale roots (R-EtOH). Caski (HPV16), HeLa (HPV18), C33A (HPV-) cervical cancer cell lines (CC) and keratinocytes HaCaT (HPV-) were used. Viability and inhibitory concentration values were determined by the MTT assay. Clonogenic assay was used to evaluate the survival. Effect on cell migration was evaluated by wound healing assay. Apoptotic response was observed using the TUNEL and Annexin V/7AAD assay.
Results

R-EtOH extract showed a dose-dependent cytotoxic effect on all cell lines. The number and size of the colonies, mainly in CC, decrease with higher concentrations of extract. R-EtOH effectively inhibits cell migration, and causes a higher percentage of apoptotic cells.
Conclusion

This study reports, for the first time, the antitumor potential of dandelion root extract (R-EtOH) on CC. Our results showed that R-EtOH affect proliferation, survival and cell migration. In addition, R-EtOH leads to apoptosis in all CC cells; thus, it is a promising extract for future studies which will allow to evaluate its usefulness in cervical cancer treatment as in other cancer types.
The Effect of Educational Intervention on Efficacy of 1% Permethrin Shampoo and 4% Dimeticone Lotion to Treat Head Lice Infestation Using Propensity Score Matching (PSM)
Authors: Asl Eslam Moradi , Saghafipour Abedin , Hamta Amir , Taheri-Kharameh Zahra , Abazari Malek , Asghari Shabnam 
Published: June 2021

Abstract
Background

Head lice are a main public health problem and the most important human ectoparasites and the use of pediculicides is the most common way to control it. One of the possible causes of treatment failure is the lack of improper application of pediculicide. The aim of this study was to assess the effect of education on efficacy of 1% permethrin or 4% dimeticone lotion to treat head lice infestation.
Methods

This quasi-experimental study was performed on 100 people infected with head lice in comprehensive urban health centers of Ardabil (intervention group) and 400 people of East Azerbaijan and West Azerbaijan (control group) provinces from April to March 2019. The data collection tool included demographic questionnaire and examination evidence recording sheet in terms of presence of hits or adult lice.
Results

The outcome of treatment included elimination of head lice infestation on days 7, and in the case of recurrence, it was considered on days 14 and 30 after treatment. Data were analyzed using R software and Generalized Estimating Equation method. GEE analysis showed there is significant difference in evaluating head lice over time.
Conclusion

Participants who received the training intervention (OR = 3.29; CI 95%: 2.21–4.88) were more likely to have a successful treatment than control group. In the case of providing proper training on the use of pediculicides and observing hygiene tips to patients with pediculosis, could help to successful treatment of pediculosis.
Effect of Hydrogen-Rich Water on Gut Microbiota in Mice With High-Fat Diet
Authors: Xie Guijiao , Liu Zelin , Wang Jiao , He Shasha , Liu Honghong , Xu Jixiong 
Published: June 2021

Abstract
Objective: To investigate the effects of hydrogen-rich water (HGRW) on the structure and composition of intestinal microflora in mice fed high-fat diets (HFDs). Materials and Methods : C57BL/6 mice were divided into four groups: (1) normal diet (CD)-normal water (W); (2) CD-HGRW; (3) HFD-W; and (4) HFD-HGRW. After 12 weeks, we sampled fasting blood glucose, lipids, transaminases, and tissue oxidative stress levels and measured body weight. High-throughput sequencing technology was used to sequence the intestinal microflora and differences in intestinal microflora were compared by group, using bioinformatics analysis. Results: Body weight, oral glucose tolerance, and blood glucose increased significantly in the HFD group compared with the CD group (P < 0.001), and malondialdehyde levels were significantly increased in the livers of mice fed a HFD (P < 0.05). Steatosis was seen in the liver parenchyma of the HFD group, and to a lesser degree in the HGRW group. The richness and diversity of intestinal flora only decreased significantly in the HFD group (P < 0.05). However, 24 genera and 26 species were significantly different between the HFD and CD subgroups of mice fed HGRW. Nine genera and five species were significantly different between the HGRW and W subgroups of mice fed a HFD. Correlations were confirmed for 10 physiological parameters; and were positively correlated with 17 genera in the HFD group; six genera were negatively correlated in the HGRW group. Importantly, Lactobacillus was closely related to tissue malondialdehyde levels. Conclusion: Oral HGRW has beneficial effects against antioxidant stress and liver damage. It may improve the diversity and structure of the intestinal flora, enhancing the relative abundance of beneficial flora.
Stunting, age at school entry and academic performance in developing countries: A systematic review and meta-analysis
Authors: Gansaonré Rabi Joël , Moore Lynne , Bleau Louis-Philippe , Kobiané Jean-François , Haddad Slim 
Published: June 2021

Abstract
IntroductionAlthough many studies have examined the associations between growth problems in infancy and age at school entry, grade repetition, school dropout and schooling level in developing country, no synthesis of the evidence has been conducted. We aim to review evidence of the effects of stunting, or height-for-age, on schooling level and schooling trajectories, defined as the combination of school entry age, grade repetition, and school dropouts.MethodsWe conducted a systematic review of studies (last update March 20, 2021) estimating that estimate the association between stunting, or height-for-age, and at least one component of the school trajectory, or schooling level, using five databases (PubMed, Embase, Education Resources Information Center (ERIC), Web of Science and PsycINFO). Study selection and data extraction were performed by two independent reviewers. Pooled effects were calculated using the generic inverse variance weighting random effect model. The studies’ risk of bias was assessed using the ROBINS-I tool for non-randomized studies.ResultsWe screened 3944 records by titles and abstracts and retained 16 for inclusion in the qualitative and meta-analysis. Meta-analysis showed that an increase in height-for-age leads to an increase in early enrollment [OR: 1.34 (95% CI: 1.07; 1.67)], a reduction in late enrollment [OR: 0.63 (95% CI: 0.51; 0.78)], an increase in schooling level [MD: 0.24 (95% CI: 0.14; 0.34)], and a reduction of school overage [OR: 0.79 (95% CI: 0.70; 0.90)]. The odds of grade repetition increased by 59% (OR = 1.59; 95% CI: 1.18; 2.14) for stunted children compared to those with no stunting.ConclusionsThis review suggests that stunting in childhood might lead to a delay in school enrollment, grade repetition, school dropout, and low schooling levels in developing countries. Future research should evaluate the effect of stunting on academic trajectories in the same population and explore the potential modification effect of socioeconomic status. The current findings suggest that policy makers need to work more to prevent stunting and to include health issues in educational policies.Systematic review registration: PROSPERO CRD42020198346
Predicting Fault Locations based on Morphometric Features of Alluvial Fans and Basins using Artificial Neural Networks
Authors: Mokarram Marzieh , Pourghasemi Hamid Reza , Tiefenbacher John P. 
Published: June 2021

Abstract
The aim of this study is to investigate the morphometry of alluvial fans located in the vicinity of the Sabzevar and Sang-Sefid faults in northeastern Iran to determine their influence on erosion Principal component analysis (PCA) was used to select the most important morphometric factors affecting erosion. The data regarding the important parameters were input into adaptive neural-fuzzy networks (ANFIS) to predict erosion rates. The asymmetric factor (Af), hypsometric integral (Hi), and basin shape (BS) indicate that most of the sub-basins are tectonically active. The results of the PCA revealed that the most important parameters affecting erosion were Af, Pf, Lf, Rf, Vf, Pb, Ab, LC, Lb, Dd, and the geological unit. The ANFIS method showed that among the soil erosion prediction models, the FCM hybrid model had the highest accuracy. It is concluded that morphometric features can be used to predict the erosion processes in the basin.
Quadrature Photonic Spatial Ising Machine
Authors: Zhang Wenjia , Sun Wencheng , Liu Yuanyuan , Liu Qingwen , Du Jiangbing , He Zuyuan 
Published: June 2021

Abstract
The mining in physics and biology for accelerating the hardcore algorithm to solve non-deterministic polynomial (NP) hard problems has inspired a great amount of special-purpose ma-chine models. Ising machine has become an efficient solver for various combinatorial optimization problems. As a computing accelerator, large-scale photonic spatial Ising machine have great advantages and potentials due to excellent scalability and compact system. However, current fundamental limitation of photonic spatial Ising machine is the configuration flexibility of problem implementation in the accelerator model. Arbitrary spin interaction is highly desired for solving various NP hard problems. Moreover, the absence of external magnetic field in the proposed photonic Ising machine will further narrow the freedom to map the optimization applications. In this paper, we propose a novel quadrature photonic spatial Ising machine to break through the limitation of photonic Ising accelerator by synchronous phase manipulation in two and three sections. Max-cut problem solution with graph order of 100 and density from 0.5 to 1 is experimentally demonstrated after almost 100 iterations. We derive and verify using simulation the solution for Max-cut problem with more than 1600 nodes and the system tolerance for light misalignment. Moreover, vertex cover problem, modeled as an Ising model with external magnetic field, has been successfully implemented to achieve the optimal solution. Our work suggests flexible problem solution by large-scale photonic spatial Ising machine.