bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytokines Activating ILCs2 in Patients With Obesity Before and After Bariatric Surgery Shortened Title: ILCs2 in Patients With Obesity
Authors: Glück Joanna , Glück Marek , Rogala Barbara , Piecuch Jerzy 
Published: April 2021

Abstract
Abstract/Purpose: Group 2 innate lymphoid cells (ILC2) are integral regulators of adipose tissue type 2 immunity and respond to epithelial signals such as IL-25, IL-33, thymic stromal lymphopoeitin (TSLP). Purpose was to assess cytokines activating ILCs2 in serum of patients with obesity and effect of bariatric surgery on these parametersMaterial and Methods: In a single-center prospective study serum IL-25, IL-33, TSLP and ST2L levels were assayed at baseline and at 6 months after bariatric surgery and correlated with anthropometric changes and metabolism parameters.Results Mean age and median of BMI of study participants were 41.9 years ± 11 and 45.6 kg/m2 (range 36.3-56.3), respectively. Six months after surgery %EWL was 43.1 ± 10.2%. Serum TSLP was significantly lower in patients with obesity both before and after surgery than in healthy controls. TSLP values before operation were significantly correlated to HbA1c%, glucose plasma level and female sex. Serum IL-25, IL-33 and ST2L levels were comparable to controls both before and after operation. Conclusions: Decreased serum levels of TSLP may be a characteristic trait for obesity, however non-modifiable by body mass surgical reduction in short time observation. Low serum levels of TSLP are related to disturbances in glucose metabolism and female sex.
Quality of life in patients with traumatic brain injury: A single tertiary center experience
Authors: Arifin Muhammad Zafrullah , Hermanto Yulius , Sutiono Agung Budi 
Published: April 2021

Abstract
Introduction Traumatic brain injury (TBI) is the main cause of death in motor-vehicle accidents. Investigation on the quality of life in patients with TBI would provide essential information for the society and policy makers in seeking the optimum ways to manage this devastating injury. Methods A total 178 of patients were involved in this study, consists of 97 patients with TBI and 81 non-TBI patients. The patients’ quality of life was evaluated by using Euroqol 5D (EQ-5D) up to 3 months of follow up. Then, the results of EQ-5D were analyzed. ResultsImpairment in mobility was more profound in patients with TBI compared to non-TBI patients (23.38% vs 9.38%, p = 0.0414). In line with the impaired mobility, impairment of self-care was also more frequently observed in patients with TBI compared to non-TBI patients (25.97% vs 12.50%, p = 0.0459). Meanwhile, the other dimensions of EQ-5D were not distinct between patients with TBI and non-TBI patients (p > 0.05). There was a trend of quality of life improvement in patients with TBI during the follow up. ConclusionPatients with TBI are more likely to experience impairment in mobility and self-care compared to non-TBI patients. Hence, TBI potentially affect quality of life.
Numerically Simulated Water Movement in Reclaimed Multi-Layered Soil Backfilled with Yellow River Sediments
Authors: Hu Zhenqi , Wang Xiatong , Liang Yusheng , Gao Yuan 
Published: April 2021

Abstract
The Yellow River interlayer filling reclamation technology can effectively improve the quality of destroyed cultivated land in the condition of limited soil resources. However, it is a conundrum to choose the appropriate sandwich strategy according to the amount of soil that can be backfilled. This study using the Hydrus-1D model to simulate water movement in reclaimed multiple-layered soils were to understand the mechanism of interlayer, and predict the optimal profile for reclaimed with Yellow River sediments. Simulations were performed on 18 soil profiles that were divided into a control check (CK) and two general scenarios that the total thickness of soil were 50 and 60 cm. Treatments in both scenarios exhibited interaction of different positions and thicknesses of soil interlayer. Results showed that removing part of the subsoil overlying the sediment placed it between sediment layers will improve the infiltration character of the conventional reconstructed soil profiles (T50-0 and T60-0). Moreover, changing the thickness of the interlayers affected infiltration character and soil water-holding capacity more than changes in the position of the layers for same total thickness of native soil. The optimal reconstructed soil profiles for scenarios 1 and 2 were T50-6 (interlayer thickness of 20 cm and located at a position of 30 cm) and T60-9 (interlayer thickness of 30 cm and located at a position of 30 cm), which could have a better infiltration character that were more closed to the native farmland.
Correlations between Serum Immunoglobulin Levels and Retinal Structural Parameters in Patients with Newly Diagnosed, Acute Vogt–Koyanagi–Harada Disease：A Retrospective Data Study
Authors: Jiang Zhijan , Zhang Nan , Ji Huiying , zhu maoli , Dong Jianhong , Zhou Min 
Published: April 2021

Abstract
background

IgE is an immune antibody involved in inflammatory response and serum IgE level elevated in some autoimmune diseases. VKH disease is autoimmune inflammatory disorder, IgE might be involved in VKH disease and high serum IgE level might related to the disease severity.
Main body

Retrospective study. Foveal thickness (FT), serous retinal detachment (SRD), sensory retinal thickness (SRT), central foveal thickness (CFT), cube volume (V), and cube average thickness (AT) were measured by OCT. According to SRD, the patients were divided into high detachment group (༞500µm) and low detachment group (≤ 500µm). Rate-scattering turbidimetry was used to measure the Immunoglobulins levels. Of 138 patients, 51 (36.96%) were included in the high detachment group. The proportion of males (2.122; 95% CI, 1.051–4.288; P = 0.035) was greater and best-corrected visual acuity (BCVA) ( -0.495; 95% CI, -0.620—-0.369; P < 0.001) was worse in the high detachment group. IgE level in the high detachment group (median: 83 IU/ml, interquartile range (IQR): 30–251) was significantly greater (P = 0.016) than that in the low detachment group (median: 53 IU/ml, IQR:32–80). FT, SRD, CFT, V, and AT were significantly greater in the high detachment group. IgE level was positively correlated with SRD, CFT, and AT. Multivariable binary logistic regression analysis showed that male (2.447; 95% CI, 1.004–5.964; P = 0.049) and serum IgE levels (0.997; 95% CI, 0.995–0.999; P = 0.014) were independent risk factors for severe SRD.
Conclusion

Male are more likely to have severe SRD, and the extent of detachment is associated with the serum IgE level. IgE may be involved in the progression of VKH disease.
Cultural adaptation of the Polish version of the PAH-SYMPACT questionnaire (PL)
Authors: Sarzyńska Kathie , Polański Jacek , Kopeć Grzegorz , Mroczek Ewa , Jankowska-Polańska Beata 
Published: April 2021

Abstract
Background: Pulmonary arterial hypertension (PAH) is a rare chronic disease that significantly affects patients' ability to perform daily activities, decreasing physical and mental well-being and social functioning. Symptoms of the disease increase as it progresses, leading to disability and death. In addition, the quality of life of patients deteriorates as the disease progresses and may reflect the effectiveness of the therapy used. Most questionnaires used in PAH clinical trials are generic quality-of-life (QoL) measures that may do not adequately reflect the clinical status and symptoms, changes in health status, or prognosis of patients with PAH. The purpose of this study was to translate and culturally adapt the Polish version of the PAH-SYMPACT questionnaire to reflect the severity of symptoms and their effects on patients’ daily functioning. Material and methods: The study included 55 patients with PAH treated, as part of a pulmonary hypertension treatment program in Poland. Validation of this questionnaire met the guidelines for translation and validation of questionnaires, which are set out in the document on the process of translation and adaptation of World Health Organization tools. Reliability was tested using a measure of internal consistency (Cronbach's α) and test-retest reliability. Correctness was confirmed using known group validity.Results: Moderate internal consistency (Cronbach's) was found for all questionnaire domains (cardiopulmonary symptoms α=0.706; cardiovascular symptoms α=0.806, physical effects α=0.904, psychological effects α=0.817) and test-retest reliability was satisfactory. The PAH-SYMPACT questionnaire (PL) met all validation criteria and can be used to assess quality of life in patients with PAH.Conclusion: Psychometric evaluation of the Polish version of the PAH-SYMPACT (PL) indicates that it is a reliable and valid measure tool to detect quality of life patients with PAH.
Green Synthesis of Silver Nanoparticles from Salvia Aethiopis L. and their Antioxidant Activity
Authors: Gecer Esma Nur 
Published: April 2021

Abstract
Salvia aethiopis L. was heated in distilled water for 2 hours. After filtration, water extract was treated with silver nitrate for 2 hours at 60°C to yield the silver nanoparticles (Sa-AgNPs). The structure of silver nanoparticles was elucidated by spectroscopic methods such as Ultraviolet-visible (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning electron microscope (SEM). The maximum absorption in UV-Vis spectrum was observed at 508 nm. XRD pattern at (2θ) 38.1°, 44.3°, 64.4°, and 77.4° degrees can be assigned to the (111), (200), (220) and (311) Bragg’s reflections of the face-centered cubic crystalline structure. The average size of Sa-AgNPs was found as 74.09 nm by SEM analysis. The characteristic hydroxyl vibration signal appeared at 3222 cm− 1. Antioxidant activity of extract and Sa-AgNPs were carried out using DPPH•, ABTS•+ FRAP assay. The Sa-AgNPs revealed the considerable ABTS•+ scavenging effect with the value of 4.93 (IC50, µg/mL) compared to BHT (IC50, µg/mL, 8.34). However, Sa-AgNPs displayed the lower DPPH• activity (IC50, µg/mL, 24.37) than that of the standard BHT (IC50, µg/mL, 9.67). The reducing power activity of Sa-AgNPs was found as 4.52 (µmol TE/mg extract) while the standard BHT value was 488 (µmol TE/mg extract).
Particle Retracking Algorithm Capable of Quantifying Large, Local Matrix Deformation for Traction Force Microscopy
Authors: Kim Sue Y. , Isogai Tadamoto , Dean Kevin , Han Sangyoon J. 
Published: April 2021

Abstract
Deformation measurement is a key process in traction force microscopy (TFM). Conventionally, particle image velocimetry (PIV) or correlation-based particle tracking velocimetry (cPTV) have been used for such a purpose. Using simulated bead images, we show that those methods fail to capture large displacement vectors and that it is due to a poor cross-correlation. Here, to redeem the potential large vectors, we propose a two-step deformation tracking algorithm that combines cPTV, which performs better for small displacements than PIV methods and a newly-designed retracking algorithm that exploits statistically confident vectors from the initial cPTV to guide the selection of correlation peak which are not necessarily the global maximum. As a result, the new method, named ‘cPTV-Retracking’, or cPTVR, was able to track more than 92% of large vectors whereas conventional methods could track 43-77% of those. Correspondingly, traction force reconstructed from cPTVR showed better recovery of large traction than the old methods. cPTVR applied on the experimental bead images has shown a better resolving power of the traction with different-sized cell-matrix adhesions than conventional methods. Altogether, cPTVR method enhances the accuracy of TFM in the case of large deformations present in soft substrates. We share this advance via our TFMPackage software.
Global Disease Burden of Uncorrected Refractive Error Among Adolescents From 1990 to 2019
Authors: Yang Zhenlan , Jin Guangming , Li Zijing , Liao Yunru , Gao Xiang , Zhang Yichi , Lan Yuqing 
Published: April 2021

Abstract
Background To estimate the global disease burden of uncorrected refractive error (URE) among adolescents and assess the contributions of various risk factors to disability-adjusted life-years (DALYs) due to URE.Methods Global, regional and country-level DALY numbers and rates due to URE among adolescents were acquired from the Global Burden of Disease Study 2019 database. Human Development Index (HDI), Socio-Demographic Index (SDI) and other country-level data were obtained from other open databases as potential indicators. Regression analysis was used to evaluate associations between DALY rates among adolescents and potential predictors.Results Global DALYs due to URE among adolescents rose by 8% between 1990 and 2019 but moderately decreased by 4.8% during this period after adjusting for population size. Female adolescents showed higher DALY rates. DALY rates sharply increased from 5 to 9 years of age, then rose more slowly, reaching a plateau before 20 years of age. Country-level DALY rates in 2019 were positively associated with HDI, SDI, and urbanization rates but negatively correlated with primary school dropout rates. Higher disease burden of adolescents visually impaired from URE was associated with lower primary school dropout rates (β=−0.257, 95% CI −0.376 to −0.138, P<0.001) and higher urbanization rates (β=0.257, 95% CI 0.067 to 0.256, P=0.001).Conclusions Higher socioeconomic status, urbanization rates and education levels are associated with a heavier disease burden of URE among adolescents. The findings of this study can provide a reference for policy making on resource allocation for URE prevention and control in teenagers.
Robust Transcriptional Regulatory Response Upon Blocking NHEJ
Authors: Benjamin Ronald , Banerjee Atoshi , Guerunk Corey , Buczek Lindsay , Eames Danielle , Trimidal Sara , Schiller Martin 
Published: April 2021

Abstract
Double strand breaks are one of the most lethal forms of DNA lesions that, if left unrepaired can lead to genomic instability, cellular transformation, and cell death. However, cells have two main machineries namely error prone Non homologous end joining repair (NHEJ) or an accurate homology dependent repair to repair the double strand breaks. NHEJ is the preferred mechanism for DNA repair and basically consists of two forms: Canonical (C-NHEJ) and Alternative (A-NHEJ) NHEJ. Our study examined the cellular repair outcome when NHEJ is blocked by targeting two key DNA repair proteins: XRCC4 and MRE-11. We developed an extrachromosomal NHEJ fluorescent reporter assay that uses Transcription activator-like effector nucleases (TALEN) to introduce double strand breaks and detect the NHEJ editing by the presence of GFP fluorescence. We demonstrated the presence of NHEJ editing in the XRCC4(-/-) cells treated with Mirin (a pharmacological inhibitor of MRE-11), albeit with a ~52% efficiency of the normal cells. The transcriptional profiles of the Mirin treated HeLa XRCC4(-/-) cells had 307 uniquely differentially expressed genes that was far greater than HeLa XRCC4(-/-) sample (83 genes) and Mirin treated HeLa cells (30 genes). Pathway analysis unique to the XRCC4(-/-) +Mirin group included differential expression of p53 downstream pathways, and metabolic pathways indicating cell adaptation for energy regulation and stress response. In conclusion, our study showed that the double strand DNA repair can be sustained even in absence of key DNA repair proteins XRCC4 and MRE-11.
Preoperative Fibrinogen and Hematological Indexes in the Differential Diagnosis of Idiopathic Granulomatous Mastitis and Breast Cancer
Authors: Velidedeoglu Mehmet , Kundaktepe Berrin Papila , Aksan Hulya , Uzun Hafize 
Published: April 2021

Abstract

Background and Aim: Studies on hematological parameters in the differential diagnosis of idiopathic granulomatous mastitis (IGM) and breast cancer (BC) are limited. This study investigated whether preoperative fibrinogen and hematological indexes can be used in the differential diagnosis of patients with IGM and early-onset BC. Methods: Fifty patients with BC, 55 patients with IGM, and 50 healthy volunteer women were included in the study. Results: There was a statistically significant difference between the IGM and the BC with respect to fibrinogen, fibrinogen/albumin (Fib/Alb) ratio, C - reactive protein (CRP), white blood cells (WBC), neutrophils, neutrophil lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and monocyte values. When fibrinogen (p <0.001), Fib/Alb ratio (p <0.001), CRP (p <0.001), WBC (p <0.001), neutrophil (p <0.001), NLR (p <0.001), monocyte (p = 0.008), and 2-hour sedimentation rate (p <0.001) were compared between the groups, the highest levels were found in the IGM group. There was a negative relationship between CRP and albumin, and a positive relationship was observed between CRP and WBC, NLR, PLR, and 2-h sedimentation rate. CRP had the highest sensitivity (95%), whereas the Fib/Alb ratio (86%) had the highest specificity. Patients with recurrent IGM had increased fibrinogen, FIB/ALB, CRP, neutrophils, NLR, and 2-h erythrocyte sedimentation rate (ESR) and decreased lymphocyte levels compared to non-recurrent patients. Conclusions: Preoperative CRP, albumin, fibrinogen, Fib/Alb, WBC, neutrophil, NLR, monocyte, and 2-h ESR have considerable potential to be early and sensitive biomarkers of IGM caused by inflammation compared to BC. These parameters also have a significant effect on the recurrence of the disease, suggesting a practical guide for the differential diagnosis of BC from IGM.
Meta-analysis of the Association Between APC Promoter Methylation and Bladder Cancer
Authors: Chen Xuedong , Li Peng , Wu Qi , Xie Fuchen 
Published: April 2021

Abstract
Bladder cancer (BC) is a worldwide disease that affects a large number of people. This study analyzes the sensitivity and specificity of adenomatous polyposis coli (APC) methylation for BC detection in urine and tissues. Combining search results from PubMed and Embase, 20 studies were included. In tissue subgroups, the OR was 6.88 (95% confidence interval (CI 2.49–19.03, P<0.001), respectively. From urine studies, the pooled OR was 4.73 at a 5% significance level (95% CI 0.87-25.66, P<0.001). In addition, analysis of the interaction between APC methylation and BC showed strong association in the whole data set. From this study, the results suggest that APC promoter methylation may be the potential testing for BC diagnosis and provide a new viewpoint in the treatment of BC.
Integrative Transcriptome Analysis Define Novel lncRNA Potential Regulation Function through SLC22A7 in Yak Liver
Authors: Xia Wei , Fu Fang , Wang Li , Luo Xiaolin , Guan Jiuqiang 
Published: April 2021

Abstract
Background: The yak (Bos grunniens) is a crucial resource to supply meat and milk to the people localized in Qinghai-Tibetan plateau area. To identify lncRNAs regulating metabolism in yak, this work adopted transcriptome method to simultaneously profile mRNAs and lncRNAs of liver in yak under three representative age (LD: Liver 1 Day, LM: Liver 15 Months, LY: Liver 5 Years) conditions.Result: Of 288 differentially expressed lncRNAs, function-oriented selection yield 88 regulated metabolically related lncRNAs that were differentially expressed at least two age conditions. These lncRNAs predicted by lncRNA-mRNA correlation analysis to function in various aspects of metabolism. Selected regulations of liver metabolically related lncRNAs were further verified by qRT-PCR. Furthermore, one novel LncRNA were selected to validate its function and result showed it potentially regulated SLC22A7, a well-known organic anion transporter.Conclusion: Combining high throughput RNA-seq screening screens, bioinformatics predictions, lncRNA-mRNA correlation analysis and qRT-PCR analysis, this study supports that a class of lncRNAs function as important metabolic regulators and establishes a foundation for further investigating the role of lncRNAs in yak.
Interactive Effects of Curcumin and Silver Nanoparticles on Growth, Hemato-Biochemical Parameters, Digestive Enzymes Activity and Histology of Common Carp (Cyprinus Carpio)
Authors: Khorshidi Zohre , Moghanlou Kourosh Sarvi , Imani Ahmad , Behrouzi Shahryar , Policar Tomas , Rahimnejad Samad 
Published: April 2021

Abstract
Background: Promising physicochemical characteristics of nanoparticles (NPs) have encouraged their increasing synthesis and application in various industries which is accompanied with their leakage to the environment with inevitable effects on non-target animals including fish. Accordingly, developing strategies to protect fish against NPs contamination has gained attention. The aims of this study were to (a) explore the effects of feed born silver nanoparticles (AgNPs) in common carp (Cyprinus carpio), and (b) to examine whether dietary curcumin supplementation can ameliorate the impacts of AgNPs on growth, hemato-biochemical parameters, digestive enzymes activity and organ histology. Methods: Nine experimental diets were prepared with three different levels of AgNPs (0, 0.05 and 0.15 g/kg) and curcumin (0, 0.75 and 1.5 g/kg) and fed to triplicate groups of common carp (4.82 ± 0.41 g) for 60 days. Results: The results showed that AgNPs significantly (P < 0.05) reduces growth performance and enhances feed conversion ratio in a dose dependent manner. Supplementing 0.75 g/kg curcumin at lower AgNPs level improved growth rate while its inclusion at higher AgNPs level further hampered fish growth. A similar trend was observed for survival rate. The highest hematocrit and hemoglobin concentrations and white blood cells count were recorded in the group received 0.75 g/kg curcumin, and inclusion of the same dose of curcumin in the diet containing 0.05 g/kg AgNPs retrieved the reduction of these parameters. Serum aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activities, and glucose, cholesterol and triglyceride concentrations were enhanced by increasing AgNPs level, and curcumin inclusion particularly at lower level of AgNPs significantly decreased their values. Activity of digestive enzymes including alkaline protease and lipase were progressively decreased by increasing AgNPs level and significant improvements were found by curcumin application at lower AgNPs level. In addition, severe pathological changes in liver and intestine were observed at high concentrations of AgNPs and curcumin. Conclusions: The findings in this study demonstrated that dietary supplementation of curcumin at lower dose of AgNPs can restrain the toxic effects, however, its inclusion at higher dose of AgNPs exacerbated the negative impacts.