bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of biofilm production and antimicrobial susceptibility pattern among urinary isolates
Authors: Joshi Pankaj A , Rajmane Ashwini , Shikhare Vishakha , Ramteerthakar Meena , Kulkarni Vanita 
Published: December 2021

Urinary tract infection (UTI) is the most commonly acquired bacterial infection. Bacterial biofilms play an important role in urinary tract infections and are responsible for persistent infections as well as higher antimicrobial resistance. The microbial biofilms pose a public health problem as the microorganisms in the biofilms are difficult to treat with antimicrobial agents. So the present study was undertaken with the aim to study biofilm production and antimicrobial susceptibility pattern of urinary isolates. Aerobic bacterial isolates from urine samples submitted to microbiology laboratory for culture were included in the study. The isolates were tested for biofilm formation by Congo red agar method and Christensen tube method. Antimicrobial susceptibility tests were performed on these isolates by Kirby Bauer disk diffusion method as per CLSI guidelines. A total of 293 Gram negative bacilli and 59 Gram positive cocci were tested for biofilm production and antimicrobial susceptibility testing. : Gram‑negative organisms were predominant (83.24%) of all the isolates. Biofilm production was detected in 47% of the isolates. 51.7%), were the most common biofilm producing Gram negative bacilli followed by (44.32%). Amongst Gram positive cocci, (77.8%) was the most common biofilm producing organism. Biofilm producing urinary isolates displayed relatively less percentage of antimicrobial susceptibility than biofilm non producers. Biofilm forming isolates showed higher antimicrobial resistance as compared to biofilm non producer. Early detection of biofilm production in urinary isolates may aid clinicians in treatment of urinary tract infections.
Erysipelothrix rhusiopathiae Prosthetic Joint Infection in an Immunocompromised Patient
Authors: Mahon John , Phoenix Eimear , Sundanum Sonia , O'Rourke Killian , O'Sullivan Cathal E , Merghani Khalid 
Published: December 2021

Case:
We report the case of an immunosuppressed 65-year-old man with prosthetic joint infection (PJI) 23 years postoperatively because of Erysipelothrix rhusiopathiae, through hematogenous seeding of cutaneous erysipeloid. Immunotherapy was discontinued, washout was performed, and antimicrobial therapy was guided by laboratory sensitivities. The patient was discharged on suppressive oral ciprofloxacin monotherapy. First-stage revision was performed at 5 months after presentation—subsequent aspiration at 1 year postoperatively demonstrated no organisms and no leucocytes. At 18-month follow-up, the patient continues to do well and has elected not to proceed with second-stage surgery.

Conclusion:

E. rhusiopathiae is a rarely seen pathogen in PJI—it should be considered with immunosuppression and relevant exposure risks. The patient achieved good clinical outcome and has experienced no sequelae to date.
Team Approach: Preoperative Management of Metabolic Conditions in Total Joint Replacement
Authors: Liu Christina , Brinkmann Elyse , Chou Sharon H. , Tejada Arias Karla , Cooper Lisa , Javedan Houman , Iorio Richard , Chen Antonia F. 
Published: December 2021

» Total joint arthroplasties (TJAs) of the knee and hip have been considered 2 of the most successful surgical procedures performed to date.» Frailty is defined as increased vulnerability to adverse outcomes with physiologic stress.» Preoperative optimization of frailty and metabolic bone conditions, including osteoporosis, vitamin D deficiency, and diabetes, through a multidisciplinary approach can help improve outcomes and minimize costs after TJA.
Assessment of Left Ventricle Myocardial Function in Hypertensive Patients Using Three-Dimensional Speckle-Tracking Echocardiography (3D-STE)
Authors: Ali Shima , Gareeballah Awadia , Yousif Rihab , Mohammed Ahmed , Mohammed Marwa , Abouraida Raga , XIE Mingxing 
Published: December 2021

Background: Increased myocardial fibrosis in hypertension leads to abnormalities in left ventricular diastolic function. 3D-speckle-tracking imaging (3D-STI) is a primary imaging modality used to detect early changes in the left ventricle (LV). The aim of this study was to assess the left ventricular myocardial function in hypertensive patients using 3D-speckle tracking imaging (3D-STI).	Methods and Results: A case control, nonintervention, descriptive study was conducted in the Department of Ultrasound Diagnosis of Union Hospital of Tongji Medical College of Huazhong University of Science and Technology (Wuhan, Hubei, China). The study subjects included 64 patients with hypertension (HT) and, as control group, 44 normotensives. HT patients were divided into HT-I group (SBP of 130-139 mmHg or DBP of 80-89 mmHg, and HT-II group (SBP >140 mmHg or DBP >90 mmHg). In this study, LV geometry and function were assessed using conventional 2D- and 3D-echocardiography in a total of 108 consecutive subjects. LV volumes, global and regional strains were measured using 3D-STI. LV ejection fraction (LVEF) was in normal range in three groups, but in general, it slightly decreased in HT-II patients, compared with control and HT-I groups (62.5±2.1%, 68.0±2.2%, and 67.5±1.3%, respectively, P=0.00). Global systolic strain demonstrated a significant decrease in GLS, GCS, and GRS in the HT-II group, compared with control and HT-I groups. All regional strain parameters (longitudinal, circumferential, and radial) significantly decreased in HT-II patients, compared with control and HT-I groups.	Conclusion: A significant deterioration of global LV systolic functions is found in hypertensive patients with well-preserved LVEF, especially in patients with hypertension stage II.
Dyslipidemia as Predictor of Missed Miscarriage
Authors: Orazmuradov Agamurad , Morozov Sergey , Akhmatova Anastasiya , Haddad Khalid , Lopatin Alexander , Ramazanova Fatima , Bekbaeva Irina , Kyrtikov Sergey , Suleymanova Zhasmina , Lukaev Aleksey 
Published: December 2021

Background: This study aimed at finding the diagnostic and prognostic possibilities of determining apoC-II, as a serological marker for MM in early gestation.	Methods and Results: The study included 182 pregnant women aged between 18 and 45 years at gestational age under 11 weeks. All women were divided into 3 groups. Group 1 (Gr1) included 90 women with MM; Group 2 (Gr2) included 52 women with spontaneous miscarriage; Group 3 included 40 women without pathology (control group [CG]). Lipid metabolism disorders were diagnosed according to the Russian national recommendations of the VII revision(the Russian Society of Cardiologists [RSC, 2020]), considering the European recommendations (2019). Proteomic analysis of the blood serum was performed using LC-MS. Abnormalities in the lipid profile were more common in patients with MM and spontaneous abortions: 62.2% and 59.7% of cases, respectively, which correlates with the identified marker apoC-II in Gr1 and Gr2.	Conclusion: ApoC-II can be considered as the most promising serologic marker for MM in the early gestation period for women with dyslipidemia.
Women’s Perception of and Readiness to Adopt a Sustainable Healthy Diet: A Cross-sectional Survey in Enugu City, Nigeria
Authors: Ogbuabor Daniel Chukwuemeka , Ogbuabor Alphonsus Ogbonna 
Published: December 2021

Abstract
Background: Perceived benefit of and readiness to adopt sustainable healthy diets (SHDs) is under-investigated in low-resource countries. We assessed women’s perceived benefit of and readiness to adopt SHDs and their associated factors in Enugu Metropolis, Nigeria. Methods: A household cross-sectional survey of childbearing women (n = 450) was conducted in January and February 2021 using a questionnaire assessing food choice motives, perceived benefit, and readiness to adopt SHDs. Readiness to adopt SHDs was grouped into pre-contemplation and contemplation (PC/C), preparation and relapse (P/R), and action and maintenance (A/M). Results: About 79% and 60% of women have high perception and adopted SHDs respectively. Perceived benefit of SHD was associated with younger age (β = -0.20, ρ < 0.05), low education (β = -0.19, ρ < 0.05), and poor wealth quintile (β = -0.57, ρ < 0.001). PC/C was predicted by low perceived benefit (OR = 10.07, 95% CI: 4.78-21.22, ρ < 0.001), low education (OR = 2.51, 95% CI: 1.25-5.04, ρ = 0.010), and taste (OR = 3.96, 95% CI: 1.61-9.75, ρ = 0.003). PR was predicted by low perceived benefit (OR = 3.92, 95% CI: 1.99-7.73, ρ < 0.001), low education (OR = 1.82, 95% CI: 1.00-3.29, ρ = 0.049). A/M was related to younger age (OR = 0.48, 95% CI: 0.27-0.84, ρ = 0.010, PR), and health (OR = 0.14, 95% CI: 0.06-0.36, ρ < 0.001, PC/C) and (OR = 0.17, 95% CI: 0.08-0.35, ρ < 0.001, P/R). Conclusions: Adoption of SHDs need to improve in Enugu, Nigeria. We identified the factors that should inform dietary guidelines and campaigns to increase women's adoption of SHDs.
Gene Co-expression Network Characterizing Microenvironmental Heterogeneity and Intercellular Communication in Pancreatic Ductal Adenocarcinoma: Implications of Prognostic Significance and Therapeutic Target
Authors: Wu Chengsi , Liu Yizhen , Cai Kun , Tao Li , Wei Dianhui , Jing Tiantian , Wang Boshi 
Published: December 2021

Abstract
Background Pancreatic ductal adenocarcinoma (PDAC) is characterized by intensive stroma involvement and heterogeneity. Pancreatic cancer cells interplay with surrounding tumor micro-environment (TME), leading to exacerbated tumorigenesis, dismal prognosis and tenacious therapy resistance. Herein, we aim to ascertain a gene-network indicative of vicious features of TME, then find a vulnerability for pancreatic cancer. Methods Single cell RNA sequencing data was processed by Seurat package, retrieving the cell component marker genes (CCMGs). Correlation networks/modules of CCMGs were determined by WGCNA algorithm in a combined PDAC mRNA expression dataset. The gene modules that statistically associate with prognosis were chosen for classifying TME subgroups, constructing neural network and designing the risk score system. Cell-cell communication analysis was achieved by NATMI software. The tumor suppressive effect of ITGA2 inhibitor was evaluated in vivo by using a Kras G12D -driven murine pancreatic cancer model.Results WGCNA analysis categorized cell component marker genes into eight co-expression networks. From gene modules with the maximum and minimum hazard ratio, we stratify PDAC samples based on TME gene patterns, resulting in two main TME subclasses with contrasting survival periods. Furthermore, we generated a neural network model and a risk score model which robustly predict prognosis and therapeutic outcomes. The hub genes in both gene modules were also gathered for functional enrichment analysis, elucidating a crucial role of cell communication-mediating integrins in TME associated PDAC malignancy. To perform a confirmatory experiment underpinning the significance of hub gene targeting, the mice with spontaneously developed pancreatic cancer were orally treated with an integrin inhibitor. The in vivo assays unraveled that pharmacologically inhibiting ITGA2 counteracts cancer-promoting micro-environment, and ameliorates pancreatic lesions. Conclusions By recapitulating gene-network across various cell types, we exploited novel PDAC prognosis-predicting strategies. Medically interfering ITGA2, a key factor guiding cellular reciprocal interaction, attenuated tumor development. These findings may open new avenue about PDAC targeting therapy.
Comprehensive Evaluation of the Effects of Long-term Cryopreservation on Peripheral Blood Mononuclear Cells using Flow Cytometry
Authors: Li Bo , Yang Chunmei , Ja Gui , Liu Yansheng , Wang Na , Yang Fangfang , Su Rui , Shang Yulong , Han Ying 
Published: December 2021

Abstract
Human peripheral blood mononuclear cells (PBMCs) originate from hematopoietic stem cells (HSCs) in the bone marrow, which mainly includes lymphocytes (T cells, B cells, and natural killer [NK] cells) and monocytes. Cryopreserved PBMCs providing biobank resources are crucial for clinical application or scientific research. Here, we used flow cytometry to explore the influence of long-term cryopreservation on the quality of PBMCs with the aim of providing important evidence for the effective utilization of biobank resources. The PBMCs were isolated from the peripheral blood, which was collected from volunteers in the hospital. After long-term cryopreservation in liquid nitrogen, we analyzed the changes in cell numbers, viability, and multiple subtypes of PBMCs and studied the apoptosis, proliferation, activation, function, and status of T cells in comparison with freshly isolated PBMCs by flow cytometry, and then further tracked the effects of long-term cryopreservation on the same sample. Although the different cell types in the PBMCs dynamically changed compared with those in the freshly isolated samples, PBMC recovery and viability remained stable after long-term cryopreservation, and the number of most innate immune cells (e.g., monocytes and B cells) was significantly reduced compared to that of the freshly isolated PBMCs or long-term cryopreserved PBMCs; more importantly, the proportion of T cell subtypes, apoptosis, proliferation, and functional T cells, except for Tregs, were not affected by long-term cryopreservation. However, the proportions of activated T, naïve T, central memory T, effector T, and effector memory T cells dynamically changed after long-term cryopreservation. This article provides important evidence for the effective utilization of biobank resources. Long-term cryopreserved PBMCs can be partly used as biological resources for clinical research or basic studies, but the effect of cryopreservation on PBMCs should be considered when selecting cell samples, especially in research relating to activating or inhibiting function.
Effect of the Trends of Estradiol Level on the Outcome of in Vitro Fertilization-embryo Transfer With Antagonist Regimens: a Single Center Retrospective Cohort Study
Authors: Wei Chun-Xiao , Zhang Liang , Pang Cong-Hui , Qi Ying-Hua , Zhang Jian-Wei 
Published: December 2021

Abstract
Background: The outcome of in vitro fertilization-embryo transfer is often determined according to follicles and estradiol levels following gonadotropin stimulation. However, there is no accurate indicator to predict pregnancy outcome, and it has not been determined how to choose subsequent drugs and dosage based on the ovarian response. This study aimed to make timely adjustments to follow-up medication to improve clinical outcomes based on the potential value of estradiol growth rate.Methods: Serum estradiol levels were measured on the day of gonadotrophin treatment (Gn0), four days later (Gn4), seven days later (Gn7), and on the trigger day (HCG). The ratio was used to determine the increase in estradiol levels. According to the ratio of estradiol increase, the patients were divided into four groups: group A1 (Gn4/Gn0≤6.44), group A2 (6.44˂Gn4/Gn0≤10.62), group A3 (10.62˂Gn4/Gn0≤21.33), and group A4 (Gn4/Gn0>21.33); group B1 (Gn7/Gn4≤2.39), group B2 (2.39˂Gn7/Gn4≤3.03), group B3 (3.03˂Gn7/Gn4≤3.84), and group B4 (Gn7/Gn4>3.84). We analyzed and compared the relationship between data in each group and pregnancy outcome.Results: In the statistical analysis, the estradiol levels of Gn4 (P = 0.029, P = 0.042), Gn7 (P< 0.001, P = 0.001), and HCG (P< 0.001, P = 0.002), as well as the ratios of Gn4/Gn0 (P = 0.004, P = 0.006), Gn7/Gn4 (P = 0.001, P = 0.002), and HCG/Gn0 (P< 0.001, P< 0.001) both had clinical guiding significance, and the lower one significantly reduced the pregnancy rate. The outcomes were positively linked to groups A (P = 0.040, P = 0.041) and B (P = 0.015, P = 0.017). The logistical regression analysis revealed that group A1 (OR = 0.440 [0.223–0.865]; P = 0.017, OR = 0.368 [0.169–0.804]; P = 0.012) and B1 (OR = 0.261 [0.126–0.541]; P< 0.001, OR = 0.299 [0.142–0.629]; P = 0.001) had opposite influence on outcomes.Conclusion: Maintaining a serum estradiol increase ratio at least above 2.39 on Gn7/Gn4 may result in a higher pregnancy rate. When estradiol growth is not ideal, gonadotrophin dosage should be adjusted appropriately to ensure the desired outcome.
Underlying Role of Rumination-Mediated Attachment Style Plays In PTSD After TIA And Stroke
Authors: Zhou Kaiping , Zhang Linjing , Li Tonggui , Wang Weiping 
Published: December 2021

Abstract
Objective: Attachment and rumination was examined as intermediary variable on post-traumatic stress disorder and medication compliance in stroke or TIA patients.Methods: 300 participants with stroke or TIA form The Second Hospital of Hebei Province were selected. Patients accomplished NIHSS, ABCD2, ECR, RSQ and RRS on admission. After 3 months the PCL-C and MMAS were collected.Results: In this stroke or TIA patients, the incident of PTSD was 7.7%; PTSD scores were significantly associated with attachment anxiety (r= 0.225，p<0.01), symptom rumination (r= 0.197, p<0.01), and obsessive thinking (r=0.187, p<0.01).After Sobel test analysis and verification by Baron and Kenny (1996) 's method, we found that ruminant mediated the relationship between attachment anxiety and PTSD; obsessive thinking mediated the relationship between attachment anxiety and PTSD.Conclusions: The relationship between attachment anxiety and PTSD was positively predicted by rumination and obsessive thinking. Adult attachment style, rumination and PTSD scores may not predict medication compliance.
Elevated Plasma Level of the Glycolysis Byproduct Methylglyoxal on Admission Is an Independent Biomarker of Mortality in ICU COVID-19 Patients
Authors: Alomar Fadhel A. , Alshakhs Marai N. , Abohelaika Salah , Almarzouk Hassan M. , Almualim Mohammed , Al-Ali Amein K. , Al-Muhanna Fahad , Alomar Mohammed F. , Alhaddad Mousa J. , Almulaify Mohammed S. , Alessa Faisal S. , Alsalman Ahmed S. , Alaswad Ahmed , Bidasee Sean R. , Alsaad Hassan , Alali Rudaynah A , AlSheikh Mona H. , Akhtar Mohammed S. , Mohaini Mohammed Al , Alsalman Abdulkhaliq J. , Alturaifi Hussain , Bidasee Keshore R. 
Published: December 2021

Abstract
Biomarkers to identify ICU COVID-19 patients at high risk for mortality are urgently needed for therapeutic care and management. In this study we found that plasma levels of the glycolysis byproduct methylglyoxal (MG) were 4.4-fold and 1.7-fold higher (P<0.0001) upon ICU admission in patients that later died (n=34) compared to uninfected controls (n=30) and those survived (n=31), respectively. The increase in MG was inversely correlated with glutathione (r2=-0.63) and the MG-glutathione degrading glyoxalase-1 (r2=-0.50), and positively correlated with the inflammation markers, SSAO (r2 =0.52), TNF-a (r2 =0.41), IL-1b (r2 =0.25), CRP (r2 =0.26) and age (r2 =0.20). Logistic regression analysis provides evidence of a significant relationship between the elevated MG upon admission into ICU and death (P<0.0001), with 42% of the death variability explained. From these data we conclude that elevated plasma MG on admission is a novel independent biomarker that predicts ICU COVID-19 patients at high risk of mortality.
Stability Analysis of Accumulation Landslides in the Three Gorges Reservoir Area Under Reservoir Water Level Fluctuation Based on Multi-Circular Model
Authors: Fan Zhiqiang , Zheng Yanhao 
Published: December 2021

Abstract
In the Three Gorges Reservoir (TGR) area, the accumulation landslide characterized by stepped slip surfaces is widely developed, and its stability is significantly affected by the fluctuation of reservoir water level. In this paper, the Shuping landslide, a typical accumulation landslide in the TGR area, was selected to study the effect of water level fluctuations on landslide stability. Based on Multi-Circular (M-C) model, it is found that the decline of reservoir water level was the dominant factor causing the decrease of landslide stability. At the end of the decline of reservoir water level, the landslide stability was minimum and the corresponding moment was the most dangerous. The effect of the drawdown speed of reservoir water level on the minimum value of landslide stability had a threshold effect, although the minimum stability coefficient of landslide decreased with the increase of drawdown speed. Under the most dangerous water level conditions, the stability of the piled landslide increased linearly with the increase of the net thrust of piles. Also, by comparing with other classical models, the effectiveness of the M-C model in evaluating landslide stability under the dynamic changes of reservoir water level was verified. The results could provide a reliable scientific basis for improving the stability analysis and reinforcement measures of the accumulation landslide with the multi-circular slip surfaces in the TGR area, as well as can be applied to similar landslides in reservoir areas.
Delineation of Geothermal Energy Potentials in Parts of Calabar Flank, Southeastern Nigeria Using Aeromagnetic Data
Authors: Omokpariola Elshalom , Anakwuba Emmanuel 
Published: December 2021

Abstract
In tackling energy-related challenges in Nigeria; the exploration of an alternative source of energy (Geothermal Energy) comes to the limelight as it is generated below the earth subsurface. This work focuses on the delineation of geothermal energy potentials in parts of Calabar Flank, in southeastern Nigeria using Aeromagnetic Data. The aim is to understand the geothermal energy potentials and structural of parts of the Calabar flank by studying the various geothermal, geological, and structural parameters using Aeromagnetic Data. The methodologies applied are quantitative for structural analysis and qualitative using spectral analysis and 3D Euler Deconvolution. The study area lies between within Latitude 5°30ʹ00ʺ N - 6°30ʹ00ʺN and Longitude 7°30ʹ00ʺE - 8°30ʹ0ʺE respectively. Results from the 3D Euler analysis revealed the depth range of 0.25 Km to 4.018 Km. the spectral Analysis revealed a depth range of Magnetic source (Zt) is (-)0.564 Km to (-)0.828 Km, the Zo is (-)4.261 Km to (-)5.999Km and the average depth to basement thickness is (-)4.825 Km. The Curie Point Depth, Geothermal Gradient, and Heat flow yield an average depth of (-)9.452 Km, a value of 61.893CKm-1, and 154.983 mWm-2 from the Spectral Analysis. Some structural features such as trending faults, and fractured basements was observed at the NE-SW of the study area and this correlated to the relatively high heat flow and geothermal gradient at the NNE-SSW part is associated with thermal structures, mineralogical and tectonic history from the NE-SW trending fault in the study area is suitable for geothermal energy exploitation.