bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serologic and molecular studies to identify neonatal alloimmune neutropenia in a cohort of 10,000 neonates
Authors: Abbas Samira A. , Lopes Larissa B. , Moritz Elyse , Martins Juliana O. , Chiba Akemi K , Kunioshi Alessandra M. , Barbosa Elisama S. , Langhi Junior Dante M. , dos Santos Amélia M. N. , Godinho Cárlei H. , Bordin José O. 
Published: February 2021

SummaryNeonatal alloimmune neutropenia (NAIN) is caused by maternal alloimmunisation to fetal human neutrophil antigens (HNAs). This study investigated maternal HNA/HLA alloantibodies involved with NAIN and identified the frequency of NAIN in Brazilian neonates. Neonatal neutropenia (neutrophil count < 1.5 × 109/L) was investigated in samples from 10,000 unselected neonates, resulting in 88 neutropenic newborns (NBs) and their 83 mothers. Genotyping was performed by PCR‐SSP (HNA‐1/‐4) and PCR‐RFLP (HNA‐3/‐5). Serologic studies were performed by GAT (granulocyte agglutination test), Flow‐WIFT (white blood cells immunofluorescence test) and LABScreen‐Multi‐HNA‐Kit (OneLambda®) (LSM). Neonatal neutropenia was identified in 88/10,000 (0·9%) NBs. Genotyping revealed 60·2% maternal‐fetal HNA incompatibilities (31·8% for HNA‐1; 14·8% for HNA‐3; 15·9% for HNA‐4; 21·6% for HNA‐5). Serologic studies revealed 37·3% of mothers with positive results with at least one technique. The detected anti‐HNA specificities were confirmed in eight positive cases related to HNA‐1/‐3 systems. In cases with maternal‐fetal HNA‐4/‐5 incompatibility, no specific neutrophil alloantibodies were found but anti‐HLA I/II were present. Anti‐HNA‐2 was not identified. This is a large Brazilian study which involved the investigation of antibodies against all five HNA systems in neutropenia cases and showed a frequency of NAIN in 8/10,000 neonates. Among the HNA antibodies identified, we highlight the anti‐HNA‐1d and anti‐HNA‐3b, antibodies unusual in alloimmunised women, and rarely related to NAIN cases.
Cord blood beyond transplantation: can we use the experience to advance all cell therapies?
Authors: Scaradavou Andromachi 
Published: February 2021

SummaryUnrelated cord blood (CB) units, already manufactured, fully tested and stored, are high‐quality products for haematopoietic stem cell transplantation and cell therapies, as well as an optimal starting material for cell expansion, cell engineering or cell re‐programming technologies. CB banks have been pioneers in the development and implementation of Current Good Manufacturing Practices for cell‐therapy products. Sharing their technological and regulatory experience will help advance all cell therapies, CB‐derived or not, particularly as they transition from autologous, individually manufactured products to stored, ‘off‐the shelf’ treatments. Such strategies will allow broader patient access and wide product utilisation.
Non‐myeloablative human leukocyte antigen‐matched related donor transplantation in sickle cell disease: outcomes from three independent centres
Authors: Alzahrani Mohsen , Damlaj Moussab , Jeffries Neal , Alahmari Bader , Singh Avani , Rondelli Damiano , Tisdale John F. , Saraf Santosh L. , Hsieh Matthew M. 
Published: February 2021

SummaryNon‐myeloablative haematopoietic progenitor cell transplantation (HPCT) from matched related donors (MRD) has been increasingly utilized in sickle cell disease (SCD). A total of 122 patients received 300 cGy of total body irradiation (TBI), alemtuzumab, unmanipulated filgrastim‐mobilized peripheral blood HPC and sirolimus. The median follow‐up was four years; median age at HPCT was 29 years. Median neutrophil and platelet engraftment occurred on day 22 and 19 respectively; 41 patients required no platelet transfusions. Overall and sickle‐free survival at one and five years were 93% and 85% respectively. Age, sex, pre‐HPCT sickle complications, ferritin and infused HPC numbers were similar between graft failure and engrafted patients. Mean donor myeloid chimaerism at one and five years post HPCT were 84% and 88%, and CD3 was 48% and 53% respectively. Two patients developed grade 1 and 2 skin graft‐versus‐host disease (GVHD) with no chronic GVHD. Median days of recipients taking immunosuppression were 489; 83% of engrafted patients have discontinued immunosuppression. Haemoglobin, haemolytic parameters and hepatic iron levels improved post HPCT. Pulmonary function testing, hepatic histology and neurovascular imaging remained stable, suggesting cessation of further sickle‐related injury. Fourteen patients had children. In this largest group of adult SCD patients, this regimen was highly efficacious, well‐tolerated despite compromised organ functions pre HPCT, and without clinically significant GVHD.
‘You can’t bullshit a bullshitter’ (or can you?): Bullshitting frequency predicts receptivity to various types of misleading information
Authors: Littrell Shane , Risko Evan F. , Fugelsang Jonathan A. 
Published: February 2021

Research into both receptivity to falling for bullshit and the propensity to produce it have recently emerged as active, independent areas of inquiry into the spread of misleading information. However, it remains unclear whether those who frequently produce bullshit are inoculated from its influence. For example, both bullshit receptivity and bullshitting frequency are negatively related to cognitive ability and aspects of analytic thinking style, suggesting that those who frequently engage in bullshitting may be more likely to fall for bullshit. However, separate research suggests that individuals who frequently engage in deception are better at detecting it, thus leading to the possibility that frequent bullshitters may be less likely to fall for bullshit. Here, we present three studies (N = 826) attempting to distinguish between these competing hypotheses, finding that frequency of persuasive bullshitting (i.e., bullshitting intended to impress or persuade others) positively predicts susceptibility to various types of misleading information and that this association is robust to individual differences in cognitive ability and analytic cognitive style.
Defining prostate cancer size and treatment margin for focal therapy: does intralesional heterogeneity impact the performance of multiparametric MRI?
Authors: Aslim Edwin Jonathan , Law Yu Xi Terence , Fook‐Chong Stephanie Man Chung , Ho Henry Sun Sien , Yuen John Shyi Peng , Lau Weber Kam On , Lee Lui Shiong , Cheng Christopher Wai Sam , Ngo Nye Thane , Law Yan Mee , Tay Kae Jack 
Published: February 2021

ObjectivesTo evaluate the impact of intralesional heterogeneity on the performance of multiparametric magnetic resonance imaging (mpMRI) in determining cancer extent and treatment margins for focal therapy (FT) of prostate cancer.Patients and MethodsWe identified men who underwent primary radical prostatectomy for organ‐ confined prostate cancer over a 3‐year period. Cancer foci on whole‐mount histology were marked out, coding low‐grade (LG; Gleason 3) and high‐grade (HG; Gleason 4–5) components separately. Measurements of entire tumours were grouped according to intralesional proportion of HG cancer: 0%, <50% and ≥50%; the readings were corrected for specimen shrinkage and correlated with matching lesions on mpMRI. Separate measurements were also taken of HG cancer components only, and correlated against entire lesions on mpMRI. Size discrepancies were used to derive the optimal tumour size and treatment margins for FT.ResultsThere were 122 MRI‐detected cancer lesions in 70 men. The mean linear specimen shrinkage was 8.4%. The overall correlation between histology and MRI dimensions was r = 0.79 (P < 0.001). Size correlation was superior for tumours with high burden (≥50%) compared to low burden (<50%) of HG cancer (r = 0.84 vs r = 0.63; P = 0.007). Size underestimation by mpMRI was more likely for larger tumours (51% for >12 mm vs 26% for ≤12 mm) and those containing HG cancer (44%, vs 20% for LG only). Size discrepancy analysis suggests an optimal tumour size of ≤12 mm and treatment margins of 5–6 mm for FT. For tumours ≤12 mm in diameter, applying 5‐ and 6‐mm treatment margins would achieve 98.6% and 100% complete tumour ablation, respectively. For tumours of all sizes, using the same margins would ablate >95% of the HG cancer components.ConclusionsMultiparametric MRI performance in estimating prostate cancer size, and consequently the treatment margin for FT, is impacted by tumour size and the intralesional heterogeneity of cancer grades.
Anti‐tumor efficacy of human anti‐c‐met CAR‐T cells against papillary renal cell carcinoma in an orthotopic model
Authors: Mori Jun‐ich , Adachi Keishi , Sakoda Yukimi , Sasaki Takahiro , Goto Shunsuke , Matsumoto Hiroaki , Nagashima Yoji , Matsuyama Hideyasu , Tamada Koji 
Published: February 2021

AbstractChimeric antigen receptor (CAR)‐T cell therapy has shown salient efficacy in cancer immunotherapy, particularly in the treatment of B cell malignancies. However, the efficacy of CAR‐T for solid tumors remains inadequate. In this study, we displayed that c‐met is an appropriate therapeutic target for papillary renal cell carcinoma (PRCC) using clinical samples, developed an anti‐human c‐met CAR‐T cells, and investigated the anti‐tumor efficacy of the CAR‐T cells using an orthotopic mouse model as pre‐clinical research. Administration of the anti‐c‐met CAR‐T cells induced marked infiltration of the CAR‐T cells into the tumor tissue and unambiguous suppression of tumor growth. Furthermore, in combination with axitinib, the anti‐tumor efficacy of the CAR‐T cells was synergistically augmented. Taken together, our current study demonstrated the potential for clinical application of anti‐c‐met CAR‐T cells in the treatment of patients with PRCC.
Developmental Improvements and Persisting Difficulties in Children’s Metacognitive Monitoring and Control Skills: Cross‐Sectional and Longitudinal Perspectives
Authors: Bayard Natalie S. , van Loon Mariëtte H. , Steiner Martina , Roebers Claudia M. 
Published: February 2021

This study investigated age‐dependent improvements of monitoring and control in 7/8‐ and 9/10‐year‐old children. We addressed prospective (judgments of learning and restudy selections) and retrospective metacognitive skills (confidence judgments and withdrawal of answers). Children (N = 305) completed a paired‐associate learning task twice, with a 1‐year delay. Results revealed improvements in retrospective, but not in prospective monitoring and control. Furthermore, control remained suboptimal, seemingly a consequence of overoptimistic monitoring. Both age groups showed stronger monitoring‐based control at the second compared to the first assessment. The comparison with a cross‐sectional sample (N = 144) revealed that improvements in retrospective monitoring can be mainly attributed to naturally occurring development, whereas retrospective control seemed to improve due to increased task familiarity.
Comparison of peri‐implant clinical outcomes of digitally customized and prefabricated abutments: A systematic review and meta‐analysis
Authors: Raee Amir , Alikhasi Marzieh , Nowzari Hessam , Djalalinia Shirin , Khoshkam Vahid , Moslemi Neda 
Published: February 2021

AbstractBackgroundDigitally customized abutments are increasingly used in contemporary implant prosthodontics.PurposeThis systematic review and meta‐analysis aimed at comparing the peri‐implant clinical outcomes of digitally customized and prefabricated abutments.Materials and methodsThe search strategies included electronic databases (PubMed, Embase, Scopus, and Cochrane clinical trials database) and related journals up to September, 2020. A qualitative and quantitative synthesis was performed on data extracted from the included studies.ResultsThree RCTs (number of patients = 120; number of dental implants = 120) and two prospective cohort studies (number of patients = 144; number of dental implants = 144) with one to three‐year follow‐up periods were included. The quantitative analyses did not demonstrate a significant difference between digitally customized and prefabricated abutments for peri‐implant pocket depth (P = 0.62), plaque index (P = 0.67), bleeding on probing (P = 0.43), keratinized mucosa width (P = 0.75), and pink aesthetic score (P = 0.30) at one‐year follow‐up visit. The qualitative analyses for marginal bone level change, calculus accumulation, implant survival rate, implant success rate, white aesthetic score, and patient‐reported outcomes did not demonstrate a significant difference between two groups during 1 to 3‐year follow‐up visits.ConclusionThe current data do not provide evidence of significant differences between two abutment fabrication methods in terms of peri‐implant clinical outcomes within short‐term period (CRD42020170807).
Customized CAD/CAM titanium meshes for the guided bone regeneration of severe alveolar ridge defects: Preliminary results of a retrospective clinical study in humans
Authors: Chiapasco Matteo , Casentini Paolo , Tommasato Grazia , Dellavia Claudia , Del Fabbro Massimo 
Published: February 2021

AbstractObjectivesTo present the results of guided bone regeneration (GBR) of atrophic edentulous ridges with customized CAD/CAM titanium meshes.Material and methodsForty‐one patients, presenting with 53 atrophic sites, were enrolled between 2018 and 2019. GBR was obtained with titanium meshes filled with autogenous bone chips and bovine bone mineral (BBM). After a mean of 7 months (range: 5–12 months), meshes were removed and 106 implants placed. After a mean of 3.5 months (range: 2–5 months), implants were uncovered and prosthetic restorations started. The outcomes were vertical and horizontal bone augmentation changes, biological complications and implant survival.ResultsOut of 53 sites, 11 underwent mesh exposure: eight of them were followed by uneventful integration of the graft, while three by partial bone loss. The mean vertical and horizontal bone gain after reconstruction was 4.78 ± 1.88 mm (range 1.00–8.90 mm) and 6.35 ± 2.10 mm (range 2.14–11.48 mm), respectively. At the time of implant placement, mean changes of initial bone gain were −0.39 ± 0.64 mm (range −3.1 to + 0.80 mm) and −0.49 ± 0.83 mm (range –3.7 to +0.4 mm), in the vertical and horizontal dimensions, respectively. Reduction of bone volume was significantly higher (p < .001 for both dimensions) in the exposed sites. The mean follow‐up of implants after loading was 10.6 ± 6.5 months (range: 2–26 months). The survival rate of implants was 100%.ConclusionCustomized titanium meshes can represent a reliable tool for GBR of severely atrophic sites, with simplification of the surgical phases.
Need for lateral bone augmentation at two narrow‐diameter implants: A prospective, controlled, clinical study
Authors: Roccuzzo Andrea , Imber Jean‐Claude , Jensen Simon Storgård 
Published: February 2021

AbstractObjectivesTo detect the potential influence of implant diameter and anatomic factors on the need for bone augmentation procedures (BAPs) when replacing congenitally missing lateral incisors (MLIs).Materials and methodsPatients with congenitally missing MLIs with a mesio‐distal distance between the canine and the central incisor of 5.9–6.3 mm received a Ø2.9 mm implant while Ø3.3 mm implants were placed when the distance was 6.4–7.1 mm. The following linear measurements were recorded using a calliper: width of the alveolar process (WAP), width of the bony alveolar ridge (WAR) and thickness of the facial bone after implant osteotomy (TFB). Guided bone regeneration was performed in case of fenestration‐ or dehiscence‐type defects or a thin TFB (<1.7 mm).ResultsFifty Ø2.9 mm and 50 Ø3.3 mm were included in 100 patients. WAP and WAR did not differ between the groups (p > .05). TFB was statistically significant larger in the Ø2.9 group (1.75 ± 0.59 mm) compared to the Ø3.3 group (1.5 ± 0.63 mm) (p = .041). Fenestration‐type defects (p = .005) and a thin facial bone wall (p = .045) was observed more frequently in the Ø3.3 compared to the Ø2.9 group. Correspondingly, BAP was indicated more frequently in the Ø3.3 compared to the Ø2.9 group (p = .017). WAP, MD and WAR were statistically significant correlated to the need for BAP (p < .001). As independent variable, only WAR influenced the probability of BAP (p < .001).ConclusionThe use of 2.9 diameter implants was correlated to a reduced frequency of BAP compared to 3.3 mm implants, without reaching a statistically significant difference. Measurement of the WAP provides the clinician useful information to predict BAP.
Objective measurement and patient‐reported evaluation of the nasal airway: Is correlation dependent on symptoms or on nasal airflow?
Authors: Xavier Rui , Azeredo‐Lopes Sofia , Menger Dirk‐Jan , de Carvalho Henrique Cyrne , Spratley Jorge 
Published: February 2021

AbstractBackgroundEvidence has shown that the sensation of nasal breathing is related to variations in nasal mucosa temperature produced by airflow. An appropriate nasal airflow is necessary for changing mucosal temperature. Therefore, the correlation between objective measurements of nasal airflow and patient‐reported evaluation of nasal breathing should be dependent on the level of nasal airflow.ObjectivesTo find if the correlation between patient‐reported assessment of nasal breathing and objective measurement of nasal airflow is dependent on the severity of symptoms of nasal obstruction or on the level of nasal airflow.MethodsThe airway of 79 patients was evaluated using NOSE score and peak nasal inspiratory flow (PNIF). Three subgroups were created based on NOSE and three subgroups were created based on PNIF level to find if correlation was dependent on nasal symptoms or airflow.ResultsThe mean value of PNIF for the 79 patients was 92.6 L/min (SD 28.1 L/min). The mean NOSE score was 48.4 (SD 24.4). The correlation between PNIF and NOSE was statistically significant (P = .03), but with a weak association between the two variables (r = −.248).Evaluation of correlation based on symptoms demonstrated a weak or very weak association in each subgroup (r = −.250, r = −.007, r = −.104). Evaluation of correlation based on nasal airflow demonstrated a very weak association for the subgroups with middle‐level and high PNIF values (r = −.190, r = −.014), but a moderate association for the subgroup with low PNIF values (r = −.404).ConclusionsThis study demonstrated a weak correlation between NOSE scores and PNIF values in patients non‐selected according to symptoms of nasal obstruction or to airflow. It demonstrated that patients with symptoms of nasal obstruction have different levels of nasal airflow and that low nasal airflow prevents the sensation of good nasal breathing. Therefore, patients with symptoms of nasal obstruction may require improving nasal airflow to improve nasal breathing sensation.
Frequency of sensitization to the individual fragrances of fragrance mix I and II according to the factors included in the MOAHLFA index
Authors: Sánchez‐Pujol María J. , Docampo‐Simón Alexandre , Mercader Pedro , González‐Pérez Ricardo , Hervella‐Garcés Marcos , Sanz‐Sánchez Tatiana , Córdoba Susana , Sánchez‐Pérez Javier , Heras‐Mendaza Felipe , Giménez‐Arnau Ana M. , Gatica‐Ortega María E. , Pastor‐Nieto María A. , Zaragoza Violeta , Carrascosa José M. , García‐Bravo Begoña , Ruiz‐González Inmaculada , Borrego Leopoldo , Sánchez‐Pedreño Paloma , de Frutos Javier O. , Armario‐Hita José C. , García‐Gavín Juan , Fernández‐Redondo Virginia , Serra Esther , Silvestre Juan F. 
Published: February 2021

AbstractBackgroundFragrances constitute the second most frequent cause of allergic contact dermatitis in Spain.ObjectivesTo determine the rate of sensitization to the individual fragrances of fragrance mix (FM) I and FM II for each of the demographic and clinical factors included in the MOAHLFA (male, occupational dermatitis, atopic dermatitis, hand dermatitis, leg dermatitis, facial dermatitis, age) index.MethodsWe conducted a 5‐year retrospective study in 23 Spanish centres. We identified the patients who had undergone patch testing with a specific fragrance series after reacting positively to fragrance markers in a baseline series. We obtained the MOAHLFA index items in this population, then calculated for each demographic and clinical factor the frequencies of sensitization to the individual fragrances of FM I and FM II.ResultsA specific fragrance series was patch tested in 1013 patients. The most frequent allergens in men, women, children, and retired people were Evernia prunastri (16%), geraniol (16.6%), isoeugenol (17.9%), and geraniol (22.4%), respectively. Citral (20.5%) and hydroxyisohexyl 3‐cyclohexene carboxaldehyde (HICC) (14.5%) were the most common allergens in occupational eczemas and were also associated with a large proportion of hand and facial dermatitis.ConclusionsFrequency of sensitization to the individual fragrances of FM I and FM II varies with age, sex, affected body region, and history of occupational or atopic dermatitis.
A scoring system to predict a prolonged length of stay after surgery for Crohn’s disease
Authors: Wickramasinghe Dakshitha , Adegbola Samuel , Sahnan Kapil , Morar Pritesh , Carvello Michele , Di Candido Francesca , Maroli Annalisa , Spinelli Antonino , Warusavitarne Janindra 
Published: February 2021

AbstractAimMany factors influence the postoperative length of stay (LOS) in Crohn's disease (CD). This study aims to identify the factors associated with a prolonged LOS after ileocolic resection (ICR) for CD and to develop a scoring system to predict the postoperative LOS in CD.MethodPatient data were collected from St Marks Hospital, London, UK, and the Humanitas Clinical and Research Center Milan, Italy, for all patients who underwent an ICR for CD from 2005 to 2017. Logistic regression was used for multivariate analysis. The scoring system was developed from the logistic regression model. The performance of the scoring system was evaluated using the area under the receiver operating characteristic curve (AUROC).ResultsA total of 628 surgeries were included in the analysis. Eighty eight surgeries were excluded due to missing data. The remaining 543 were divided into two cohorts for the development (n = 418) and validation (n = 125) of the scoring system. The regression model was statistically significant (p < 0.0001). The statistically significant independent variables included the time since diagnosis, American Society of Anesthesiologists (ASA) grade, perioperative use of steroids, surgical access, strictureplasty and platelet count. The AUROCs for the development and validation cohorts were 0.732 and 0.7, respectively (p < 0.0001). The cut‐off score suggested by Youden's index was 50, with a sensitivity of 65.6% and a specificity of 73.3%.ConclusionThe time since diagnosis, ASA grade, steroid use, surgical access, strictureplasty and platelet count were associated with a prolonged LOS and were used to develop a scoring system. The calculator is available online at https://rebrand.ly/Crohnscal.
A wolf in fox's clothing? Using stable isotopes to quantify ecological replacement
Authors: Clark T. J. , Vick Bugge , Newton Jason , Marengo Ilaria , Wakefield Ewan D. 
Published: February 2021

AbstractEcological replacement as a conservation tool presupposes that nonnative taxa can restore degraded ecosystems by performing the ecological functions of extinct taxa. This assumption is rarely tested however, largely because it is difficult to quantify the functions of species extirpated long ago. Here, we test whether feral South American grey foxes (SAGF), introduced to the Falkland Islands ∼90 years ago, act as unintended ecological replacements for endemic Falkland Islands wolves (FIW), extirpated during the 19th century. Using hair stable isotope ratios as proxies for diet, we show that the isotopic niche space of modern SAGFs almost completely encompasses that of archaic FIWs. However, the former's niche is larger so while SAGFs may play similar ecological roles to FIWs, they probably perform additional functions, which may or may not be desirable. In so doing, we illustrate a generalized framework for using comparative isotopic niche analysis to test for ecological replacement objectively.