bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-chain noncoding RNA-GAS5/hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2
Authors: Zhuo Xiaozhen , Bai Kai , Wang Yingxian , Liu Peining , Xi Wen , She Jianqing , Liu Junhui 
Published: March 2021

Abstract
Objective : Diabetic cardiomyopathy (DCM) is one of the complications experienced by patients with diabetes. In recent years, long noncoding RNAs (lncRNAs) have been investigated because of their role in the progression of various diseases, including DCM. The purpose of the present study was to explore the role of lncRNA GAS5 in high glucose (HG)-induced cardiomyocyte injury and apoptosis.
Materials and methods: We constructed HG-induced AC16 cardiomyocytes and a streptozotocin (STZ)-induced rat diabetes model. GAS5 was overexpressed and knocked out at the cellular level, and GAS5 was knocked down by lentiviruses at the animal level to observe its effect on myocardial injury. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of GAS5. Cell proliferation and apoptosis after GAS5 knockout were detected by CCK-8, TUNEL, and flow cytometry assays. ELISA was used to detect the changes in myocardial enzyme content in cells and animal myocardial tissues during the action of GAS5 on myocardial injury.
Results: GAS5 expression was up-regulated in HG-treated AC16 cardiomyocytes and the rat diabetic myocardial injury model. The down-regulation of GAS5 could inhibit HG-induced myocardial damage. This work proved that the down-regulation of GAS5 could reverse cardiomyocyte injury and apoptosis by targeting miR-138 to down-regulate CYP11B2.
Conclusion: We confirmed for the first time that the down-regulation of GAS5 could reverse CYP11B2 via the miR-138 axis to reverse HG-induced cardiomyocyte injury. This research might provide a new direction for explaining the developmental mechanism of DCM and potential targets for the treatment of myocardial injury.
Brain ACE2 activation following brain aminopeptidase A blockade by firibastat in salt-dependent hypertension
Authors: Hmazzou Reda , Marc Yannick , Flahault Adrien , Gerbier Romain , De Mota Nadia , Llorens-Cortes Catherine 
Published: March 2021

Abstract
In the brain, aminopeptidase A (APA), a membrane-bound zinc metalloprotease, generates angiotensin III from angiotensin II. Brain angiotensin III exerts a tonic stimulatory effect on the control of blood pressure (BP) in hypertensive rats and increases vasopressin release. Blocking brain angiotensin III formation by the APA inhibitor prodrug RB150/firibastat normalizes arterial BP in hypertensive deoxycorticosterone acetate (DOCA)-salt rats without inducing angiotensin II accumulation. We therefore hypothesized that another metabolic pathway of brain angiotensin II, such as the conversion of angiotensin II into angiotensin 1-7 (Ang 1-7) by angiotensin-converting enzyme 2 (ACE2) might be activated following brain APA inhibition. We found that the intracerebroventricular (icv) administration of RB150/firibastat in conscious DOCA-salt rats both inhibited brain APA activity and induced an increase in brain ACE2 activity. Then, we showed that the decreases in BP and vasopressin release resulting from brain APA inhibition with RB150/firibastat were reduced if ACE2 was concomitantly inhibited by MLN4760, a potent ACE2 inhibitor, or if the Mas receptor (MasR) was blocked by A779, a MasR antagonist. Our findings suggest that in the brain, the increase in ACE2 activity resulting from APA inhibition by RB150/firibastat treatment, subsequently increasing Ang 1-7 and activating the MasR while blocking angiotensin III formation, contributes to the antihypertensive effect and the decrease in vasopressin release induced by RB150/firibastat. RB150/firibastat treatment constitutes an interesting therapeutic approach to improve BP control in hypertensive patients by inducing in the brain renin–angiotensin system, hyperactivity of the beneficial ACE2/Ang 1-7/MasR axis while decreasing that of the deleterious APA/Ang II/Ang III/ATI receptor axis.
Autism-Centered Therapy for Childhood Apraxia of Speech (ACT4CAS): A Single-Case Experimental Design Study
Authors: Beiting Molly , Maas Edwin 
Published: March 2021

Purpose
A subset of children with autism spectrum disorder (ASD) has speech sound disorders, including childhood apraxia of speech (CAS). To date, virtually all speech treatment studies consider ASD an exclusionary criterion, resulting in little scientific evidence for treatment of CAS for children who also have ASD. This study proposes and tests a novel approach, Autism-Centered Therapy for Childhood Apraxia of Speech (ACT4CAS), as a theoretically and clinically informed speech treatment option for this population.

Method
Using a multiple-baseline design within and across participants, three children with co-occurring ASD and CAS received 11–18 treatment sessions. Treatment targets were individually designed and matched with untreated control words. Probes were administered at the start of each session to assess speech production accuracy perceptually. Changes in production accuracy were examined through visual inspection and quantified with effect sizes.

Results
Findings were mixed, with one child showing significant gains for half of the treated targets at follow-up and two children showing no clear improvement.

Conclusions
Preliminary evidence suggests potentially positive treatment effects for ACT4CAS when implemented as intended, although treatment intensity and disorder severity likely influence treatment outcome. Replication and comparison of ACT4CAS to other speech treatments is needed.

Supplemental Material

https://doi.org/10.23641/asha.14110445
Obesity and Pregnancy. Guideline of the German Society of Gynecology and Obstetrics (S3-Level, AWMF Registry No. 015-081, June 2019)
Authors: Schaefer-Graf Ute , Ensenauer Regina , Gembruch Ulrich , Groten Tanja , Flothkötter Maria , Hennicke Julia , Köhrle Josef , Möhler Jantke , Kühnert Maritta , Schmittendorf Annett , Stroh Christine , Wirth Alfred , Schmidt Markus 
Published: March 2021

Abstract
Aims Obesity is an increasing problem, even in young women of reproductive age. Obesity has a negative impact on conception, the course of pregnancy, and neonatal outcomes. Caring for obese pregnant women is becoming an increasingly important aspect of standard prenatal care. This guideline aims to improve the care offered to obese pregnant women.
Methods This S3-guideline was compiled following a systemic search for evidence and a structured process to achieve consensus.
Recommendations Evidence-based recommendations for the care of obese pregnant women were developed, which cover such as areas as preconception counselling, identification of risks, special aspects of prenatal care and prenatal diagnostic procedures, intrapartum management, and long-term effects on mother and child.
Population Pharmacokinetic Analysis of Fevipiprant in Healthy Subjects and Asthma Patients using a Tukey’s g-and-h Distribution
Authors: Wang Xinting , Bartels Christian , Kulkarni Swarupa , Sangana Ramachandra , Jain Monish , Zack Julia , Yu Jing 
Published: March 2021

Abstract
Aim The objective of this analysis was to characterize the population pharmacokinetics (PK) of fevipiprant in asthma patients and to evaluate the effect of baseline covariates on the PK of fevipiprant.
Methods PK data from 1281 healthy subjects or asthma patients were available after single or once daily dosing of fevipiprant. Population PK analysis was conducted to describe fevipiprant plasma concentration data using a non-linear mixed effect modeling approach.
Results Fevipiprant PK was described by a two-compartment model with first-order absorption and first-order elimination. Exploration of fevipiprant PK in the population from the phase III studies revealed an over-dispersed and skewed distribution. This unusual distribution was described using Tukey’s g-and-h distribution (TGH) on the between-subject variability of apparent clearance (CL/F). The model identified a significant impact of disease status on CL/F, with the value in healthy subjects being 62% higher than that in asthma patients. Bodyweight, age and renal function showed statistically significant impact on fevipiprant clearance; however, compared with a typical asthma patient, the simulated difference in steady-state exposure was at most 16%.
Conclusion Fevipiprant PK was described by a two-compartment model with first-order absorption and first-order elimination. The TGH distribution was appropriate to describe the over-dispersed and skewed PK data as observed in the current studies. Asthma patients had approximately 37% higher exposure than healthy subjects did. Other covariates changed exposure by at most 16%.
Strategies of In Situ Generated Magnesium Catalysis in Asymmetric Reactions
Authors: Yang Dongxu , Wang Linqing 
Published: March 2021

AbstractMagnesium (Mg) is a cheap, non-toxic, and recyclable alkaline earth metal that constitutes about 2% weight in the Earth’s crust. The use of magnesium catalysts to forge chiral moieties in molecules is highly attractive. Based on our work in recent years, we describe the current progress in the development of in situ generated magnesium catalysts and their application in asymmetric synthesis. In this perspective, a critically concise classification of in situ generated magnesium catalytic modes, with relevant examples, is presented, and representative mechanisms of each category are discussed. Building on the established diverse strategies, one can foresee that more innovative and structurally creative magnesium catalysts that are generated in situ will be developed to overcome more formidable challenges of catalytic enantioselective reactions.1	Introduction2	Magnesium Catalysts Generated in Situ from Chiral Ligands Containing Dual Reactive Hydrogens3	Magnesium Catalysts Generated in Situ from Monoanionic Chiral	Ligands4	Bimetallic and Polymetallic Magnesium Catalysts Assembled in Situ5	Summary and Outlook
Research on photosensitive gate ferroelectric integrated GaN HEMT photodetector
Authors: Zhu Yanxu , Li Qixuan , Yang Zhuang , Wang Cai , Wei Zhao 
Published: March 2021

In this paper, we take advantage of the high sensitivity of two-dimensional electron gas concentration in the heterojunction channel of a GaN high electron mobility transistor (HEMT) to device surface states and gate voltages. By integrating a HEMT with ferroelectric materials with photovoltaic effects, a photo-sensitive gate HEMT photodetector based on ferroelectric integration is obtained. By exploring the ferroelectric and composite film sputtering growth epitaxial atmosphere, a high-performance PZT/ZnO composite ferroelectric film grown in an oxygen-containing atmosphere is obtained. Comparing the PZT and LiNbO3 (LN) ferroelectric thin films prepared with or without the buffer layer, the following conclusions are obtained. The quantum efficiency of the PZT/ZnO film increases by 240% and 596% at the peak of 300–400 nm, reaching 14.55%; the residual polarization of the PZT film obtained in an oxygen-containing atmosphere reaches 52.31 μC/cm2; the PZT/ZnO composite film has better fatigue characteristics. The GaN HEMT detector prepared by using magnetron sputtered PZT/ZnO as the sensing gate under the oxygen atmosphere has a photocurrent increment of 11.51 mA under ultraviolet light and a responsivity of 111A/W at 365 nm. At the same time, the device has τr = 0.12 s and τf = 8.3 s transient response. The research in this paper shows that a new structure photodetector based on a GaN HEMT has excellent ultraviolet light response, which provides a new research direction for the light detection mechanism.
Cytokine receptor clustering in sensory neurons with an engineered cytokine fusion protein triggers unique pain resolution pathways
Authors: Prado Judith , Westerink Remco H. S. , Popov-Celeketic Jelena , Steen-Louws Cristine , Pandit Aridaman , Versteeg Sabine , van de Worp Wouter , Kanters Deon H. A. J. , Reedquist Kris A. , Koenderman Leo , Hack C. Erik , Eijkelkamp Niels 
Published: March 2021

Significance
Interactions between the immune system and nervous system regulate chronic pain. Cytokines are well-known immune-regulatory molecules that have activities beyond regulation of the immune system, including regulation of pain pathways. We identified that fusion of regulatory cytokines interleukin (IL)-4 and IL-10 into the fusion protein IL4–10 FP provides unexpected properties to inhibit pain by clustering the respective receptor chains of both cytokines in sensory neurons. IL4–10 FP promotes unique signaling pathways and gene-expression profiles that resolve chronic inflammatory pain. Thus IL4–10 FP is an immune biologic that effectively inhibits pain by activating pain-resolution pathways in sensory neurons through clustering of cytokine receptors. This unique effect discriminates IL4–10 FP from a combination therapy with individual regulatory cytokines.
Primary differentiated respiratory epithelial cells respond to apical measles virus infection by shedding multinucleated giant cells
Authors: Lin Wen-Hsuan W. , Tsay Annie J. , Lalime Erin N. , Pekosz Andrew , Griffin Diane E. 
Published: March 2021

Significance
Measles virus (MeV) is highly infectious by the respiratory route, but respiratory epithelial cells are thought to be resistant to infection through the exposed apical surface and to require infection through the basolateral surface. We show that differentiated respiratory epithelial cells are actually highly susceptible to apical infection and that infection leads to formation of multinucleated giant cells that are shed from the epithelial sheet into the lumen leaving the epithelium intact and devoid of infected cells. These results are consistent with the hypothesis that MeV infection is initiated in respiratory epithelial cells with subsequent infection of lymphoid tissue and systemic spread.
Ancient noeggerathialean reveals the seed plant sister group diversified alongside the primary seed plant radiation
Authors: Wang Jun , Hilton Jason , Pfefferkorn Hermann W. , Wang Shijun , Zhang Yi , Bek Jiri , Pšenička Josef , Seyfullah Leyla J. , Dilcher David 
Published: March 2021

Significance
There were two heterosporous lignophyte lineages of which only one, the seed plants, survived the Permian–Triassic mass extinction. Based on exceptionally complete fossil trees from a 300-My-old volcanic ash, the enigmatic Noeggerathiales are now recognized as belonging to the other lineage. They diversified alongside the primary seed plant radiation and constitute seed plants’ closest relatives. Noeggerathiales are reconstructed as members of a plexus of free-sporing woody plants called progymnosperms, extending their age range by 60 My. Following the origin of seed plants, progymnosperms were previously thought to have become gradually less abundant before dying out in Carboniferous. We show they diversified and evolved complex morphologies including cone-like structures from modified leaves before going extinct at the Permian–Triassic extinction.
Historic Yangtze flooding of 2020 tied to extreme Indian Ocean conditions
Authors: Zhou Zhen-Qiang , Xie Shang-Ping , Zhang Renhe 
Published: March 2021

Significance
Summer rainfall along the Yangtze River in 2020 was the heaviest since 1961, with devastating socioeconomic impacts. While official forecasts based on tropical Pacific state failed, we show that dynamic models, when initialized with ocean observations globally, succeed in predicting the extreme rainfall. Slowly propagating oceanic Rossby waves in the South Indian Ocean are the source of predictability, which are in turn tied to the record-breaking Indian Ocean Dipole in late 2019. The identification of antecedent subsurface conditions of the Indian Ocean as a key predictor represents an important conceptual advance in Asian summer monsoon dynamics, helping improve disaster preparation that saves lives and properties.
Photoinduced hole hopping through tryptophans in proteins
Authors: Záliš Stanislav , Heyda Jan , Šebesta Filip , Winkler Jay R. , Gray Harry B. , Vlček Antonín 
Published: March 2021

Significance
Electron (hole) hopping through tryptophan residues activates protein cofactors, participates in cellular photosignaling, and protects enzymes from oxidative degradation. The energetics of excited chromophores, together with their positions relative to proximal indoles, are evolution-optimized in natural photolyases and cryptochromes. Our theoretical analysis of photoinduced hole hopping through tryptophans in rhenium-modified blue copper proteins has shed light on the roles of electronic coupling and adiabaticity, as well as electrostatic-field fluctuations and solvation dynamics in driving charge transport rapidly over long distances. The take-home message is that attention should be paid to solvation of redox-active molecules in hopping chains in the design of bioinspired light-harvesting systems and functional photocatalysts.
Closed-orbit theory for the photodetachment rate in a static magnetic field transversely superimposed to an oscillatory electric field
Authors: Chen X J , Titimbo K , Du M L 
Published: March 2021

Abstract
The closed-orbit theory is used for the study of the photodetachment rate of electrons from single-charged anions in presence of a homogeneous magnetic field that is perpendicular to a time-dependent electric field. The photodetachment process in the near-threshold region is achieved by a weak laser field. We describe in detail the semiclassical method to derive the general formula for the instant photodetachment rate in time-dependent systems. We find that the photodetachment rate is affected by the presence of the static magnetic field since, within the semiclassical picture, it contributes to fold back the electron trajectories back to their emission position: generating closed orbits. For weak magnetic fields, however, the number of closed orbits does not change, then the modulation of the photodetachment rate is virtually unaffected when it is compared to the case with no magnetic field. On the contrary, the modulation is clearly distinctive and complicated for strong magnetic field intensities due to the contribution of a larger number of closed orbits. Despite we provide general formulas, numerical experiments and discussions are focused on the emission of electrons as s-waves and p

z
-waves.
Physical and chemical properties of a magnetic-assisted DC superimposed nanosecond-pulsed streamer discharge plasma
Authors: Jiang Nan , Lu Xiaoling , Peng Bangfa , Li Jie , Shang Kefeng , Lu Na , Wu Yan 
Published: March 2021

Abstract
In the present work, a magnetic-assisted DC superimposed nanosecond-pulsed streamer discharge (DC-NPSD) with a 0.4 T parallel magnetic field is developed that achieves good performance for ozone production and toluene degradation. The influence of the assisted parallel magnetic field on the electrical characteristics, streamer propagation behavior, reactive species generation and plasma chemical properties of the DC-NPSD are systematically investigated. The experimental results indicate that better impedance matching of a nanosecond pulsed power supply and a discharge reactor can be realized by superimposing DC voltage (U
DC), which facilitates reactive species production and toluene degradation. The discharge current, input energy and reactive species production can be further enhanced by the application of a parallel magnetic field under different pulse and DC voltage conditions. There are two distinct streamer phases in the DC-NPSD: a primary streamer (PS) with longer propagation distance and higher propagation velocity and a secondary streamer (SS) with shorter propagation distance and lower propagation velocity. The propagation velocities of both the PS and the SS increase with increasing U
DC. Only PS propagation velocity is accelerated by a parallel magnetic field; however, that of the SS remains almost constant with or without a magnetic field. Both ozone generation and toluene degradation performance are improved by a magnetic field, which is attributed to the lengthened electron motion path under the action of Lorentz force and the constraint effect on energetic electrons in the presence of a parallel magnetic field.
Direct excitation of the magnetisation in photon-magnon hybrid systems with an infrared laser pulse
Authors: Braggio Caterina , Carugno Giovanni , Crescini Nicolò , Di Vora Raffaele , Ortolan Antonello , Ruoso Giuseppe 
Published: March 2021

Abstract
We present experimental results concerning the direct excitation of the magnetisation in a photon-magnon hybrid system composed of a microwave cavity and an embedded yttrium iron garnet (YIG) sphere. An 11 ps ultrafast pulsed laser, with wavelength of 1064 nm outside the YIG transparence window, directly excite the magnon modes. We measure the energy deposited in the Kittel mode of magnetisation by exploiting its coupling to the TE102 mode of the rectangular microwave cavity in the strong coupling regime. Energy collection is performed by a standard rf detection chain reading an antenna matched to the cavity resonance. This technique can prove to be essential in the study of the dynamics of cavity magnon-polaritons, finding application in dark matter axion searches and future magnon based quantum information studies.
Defect-engineered three-dimensional vanadium diselenide microflowers/nanosheets on carbon cloth by chemical vapor deposition for high-performance hydrogen evolution reaction
Authors: Miao Chengcheng , Zhang Ting , Li Fulin , Zhang Lei , Sun Jiamin , Liu Dong , Wu Liqian , Wang Hang , Chen Fenghua , He Longbing , Han Ning , Ma Yandong , Dai Ying , Yang Zai-xing 
Published: March 2021

Abstract
In the past decades, defect engineering has become an effective strategy to significantly improve the hydrogen evolution reaction (HER) efficiency of electrocatalysts. In this work, a facile chemical vapor deposition (CVD) method is firstly adopted to demonstrate defect engineering in high-efficiency HER electrocatalysts of vanadium diselenide nanostructures. For practical applications, the conductive substrate of carbon cloth (CC) is selected as the growth substrate. By using a four-time CVD method, uniform three-dimensional microflowers with defect-rich small nanosheets on the surface are prepared directly on the CC substrate, displaying a stable HER performance with a low Tafel slope value of 125 mV dec−1 and low overpotential voltage of 295 mV at a current density of 10 mA cm−2 in alkaline electrolyte. Based on the results of x-ray photoelectron spectra and density functional theory calculations, the impressive HER performance originates from the Se vacancy-related active sites of small nanosheets, while the microflower/nanosheet homoepitaxy structure facilitates the carrier flow between the active sites and conductive substrate. All the results present a new route to achieve defect engineering using the facile CVD technique, and pave a novel way to prepare high-activity layered electrocatalysts directly on a conductive substrate.