bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic and prognostic impact of cytokeratin 18 expression in human tumors: a tissue microarray study on 11,952 tumors
Authors: Menz Anne , Weitbrecht Timo , Gorbokon Natalia , Büscheck Franziska , Luebke Andreas M. , Kluth Martina , Hube-Magg Claudia , Hinsch Andrea , Höflmayer Doris , Weidemann Sören , Fraune Christoph , Möller Katharina , Bernreuther Christian , Lebok Patrick , Clauditz Till , Sauter Guido , Uhlig Ria , Wilczak Waldemar , Steurer Stefan , Minner Sarah , Burandt Eike , Krech Rainer , Dum David , Krech Till , Marx Andreas , Simon Ronald 
Published: February 2021

Abstract
Background
Cytokeratin 18 (CK18) is an intermediate filament protein of the cytokeratin acidic type I group and is primarily expressed in single-layered or “simple” epithelial tissues and carcinomas of different origin.

Methods
To systematically determine CK18 expression in normal and cancerous tissues, 11,952 tumor samples from 115 different tumor types and subtypes (including carcinomas, mesenchymal and biphasic tumors) as well as 608 samples of 76 different normal tissue types were analyzed by immunohistochemistry in a tissue microarray format.

Results
CK18 was expressed in normal epithelial cells of most organs but absent in normal squamous epithelium. At least an occasional weak CK18 positivity was seen in 90 of 115 (78.3%) tumor types. Wide-spread CK18 positivity was seen in 37 (31.9%) of tumor entities, including adenocarcinomas of the lung, prostate, colon and pancreas as well as ovarian cancer. Tumor categories with variable CK18 immunostaining included cancer types arising from CK18 positive precursor cells but show CK18 downregulation in a fraction of cases, tumor types arising from CK18 negative precursor cells occasionally exhibiting CK18 neo-expression, tumors derived from normal tissues with variable CK18 expression, and tumors with a mixed differentiation. CK18 downregulation was for example seen in renal cell cancers and breast cancers, whereas CK18 neo-expression was found in squamous cell carcinomas of various origins. Down-regulation of CK18 in invasive breast carcinomas of no special type and clear cell renal cell carcinomas (ccRCC) was related to adverse tumor features in both tumors (p ≤ 0.0001) and poor patient prognosis in ccRCC (p = 0.0088). Up-regulation of CK18 in squamous cell carcinomas was linked to high grade and lymph node metastasis (p < 0.05). In summary, CK18 is consistently expressed in various epithelial cancers, especially adenocarcinomas.

Conclusions
Down-regulation or loss of CK18 expression in cancers arising from CK18 positive tissues as well as CK18 neo-expression in cancers originating from CK18 negative tissues is linked to cancer progression and may reflect tumor dedifferentiation.
Interfacial Bonding Mechanism and Mechanical Performance of Continuous Fiber Reinforced Composites in Additive Manufacturing
Authors: Fan Congze , Shan Zhongde , Zou Guisheng , Zhan Li , Yan Dongdong 
Published: February 2021

AbstractThe additive manufacturing of continuous fiber composites has the advantage of a high-precision and efficient forming process, which can realize the lightweight and integrated manufacturing of complex structures. However, many void defects exist between layers in the printing process of additive manufacturing; consequently, the bonding performance between layers is poor. The bonding neck is considered a key parameter for representing the quality of interfacial bonding. In this study, the formation mechanism of the bonding neck was comprehensively analyzed. First, the influence of the nozzle and basement temperatures on the printing performance and bonding neck size was measured. Second, CT scanning was used to realize the quantitative characterization of bonding neck parameters, and the reason behind the deviation of actual measurements from theoretical calculations was analyzed. When the nozzle temperature increased from 180 to 220 °C, CT measurement showed that the bonding neck diameter increased from 0.29 to 0.34 mm, and the cross-sectional porosity reduced from 5.48% to 3.22%. Finally, the fracture mechanism was studied, and the influence of the interfacial bonding quality on the destruction process of the materials was determined. In conclusion, this study can assist in optimizing the process parameters, which improves the precision of the printing parts and performance between the layers.
Effects of an innovative densification process on mechanical and physical properties of beech and Norway spruce veneers
Authors: Cencin Alex , Zanetti Michela , Urso Tiziana , Crivellaro Alan 
Published: February 2021

AbstractIncreased wood density is obtained by compressing the wood porous structure under suitable moisture and temperature conditions to improve its physical, mechanical and color properties. A recently proposed wood densification method based on partial removal of lignin and hemicellulose in hot water solution of sodium hydroxide and sodium sulphite has shown promising results on solid wood. However, its applicability and effect on thin wood veneers have not been tested yet. In this study, the timing of the method has been adapted to estimate the densification treatment intensity dependence of wood properties (wood density and modulus of elasticity) and color change of softwood (Norway spruce) and hardwood (beech) veneers. Compared to control, density and rigidity increased, with improved wood properties peaking after only 90 s of treatment intensity. Furthermore, the color became darker after treatment compared to control, with no significant color difference between treatment intensities. In conclusion, densification of veneers, according to the presented adapted method, provides a significant improvement of veneers physical and mechanical properties, and produces color changes perceptible by the human eye. Our results can be further implemented and adapted to application in industrial plants, calling for new application of densified veneers.
Wage gains from foreign ownership: evidence from linked employer–employee data
Authors: Köllő János , Boza István , Balázsi László 
Published: February 2021

AbstractWe compare the wages of skilled workers in multinational enterprises (MNEs) versus domestic firms, the earnings of domestic firm workers with past, future and no MNE experience, and estimate how the presence of ex-MNE peers affects the wages of domestic firm employees. The analysis relies on monthly panel data covering half of the Hungarian population and their employers in 2003–2011. We identify the returns to MNE experience from changes of ownership, wages paid by new firms of different ownership, and the movement of workers between enterprises. We find high contemporaneous and lagged returns to MNE experience and significant spillover effects. Foreign acquisition has a moderate wage impact, but there is a wide gap between new MNEs and domestic firms. The findings, taken together, suggest that MNE employees accumulate partly transferable knowledge, valued in the high-wage segment of the local economy that is connected with the MNEs via worker turnover.
Accuracy of breeding values for production traits in turkeys (Meleagris gallopavo) using recursive models with or without genomics
Authors: Abdalla Emhimad A. , Wood Benjamin J. , Baes Christine F. 
Published: February 2021

Abstract
Background
Knowledge about potential functional relationships among traits of interest offers a unique opportunity to understand causal mechanisms and to optimize breeding goals, management practices, and prediction accuracy. In this study, we inferred the phenotypic causal networks among five traits in a turkey population and assessed the effect of the use of such causal structures on the accuracy of predictions of breeding values.

Methods
Phenotypic data on feed conversion ratio, residual feed intake, body weight, breast meat yield, and walking score in addition to genotype data from a commercial breeding population were used. Causal links between the traits were detected using the inductive causation algorithm based on the joint distribution of genetic effects obtained from a standard Bayesian multiple trait model. Then, a structural equation model was implemented to infer the magnitude of causal structure coefficients among the phenotypes. Accuracies of predictions of breeding values derived using pedigree- and blending-based multiple trait models were compared to those obtained with the pedigree- and blending-based structural equation models.

Results
In contrast to the two unconditioned traits (i.e., feed conversion ratio and breast meat yield) in the causal structures, the three conditioned traits (i.e., residual feed intake, body weight, and walking score) showed noticeable changes in estimates of genetic and residual variances between the structural equation model and the multiple trait model. The analysis revealed interesting functional associations and indirect genetic effects. For example, the structural coefficient for the path from body weight to walking score indicated that a 1-unit genetic improvement in body weight is expected to result in a 0.27-unit decline in walking score. Both structural equation models outperformed their counterpart multiple trait models for the conditioned traits. Applying the causal structures led to an increase in accuracy of estimated breeding values of approximately 7, 6, and 20% for residual feed intake, body weight, and walking score, respectively, and different rankings of selection candidates for the conditioned traits.

Conclusions
Our results suggest that structural equation models can improve genetic selection decisions and increase the prediction accuracy of breeding values of selection candidates. The identified causal relationships between the studied traits should be carefully considered in future turkey breeding programs.
Genetic diversity analysis of brown marmorated stink bug, Halyomorpha halys based on mitochondrial COI and COII haplotypes
Authors: Yan Juncong , Pal Chandan , Anderson Diane , Vétek Gábor , Farkas Péter , Burne Allan , Fan Qing-Hai , Zhang Jinping , Gunawardana Disna N. , Balan Rebijith Kayattukandy , George Sherly , Li Dongmei 
Published: February 2021

AbstractBackgroundIn the past decade, the brown marmorated stink bug (BMSB),Halyomorpha halys(Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries.ResultIn total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity (Hd) and nucleotide diversity (π) were high while the neutrality (Fu’s Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu’s Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu’s Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exists among the BMSB populations from China, Japan, and the invasive countries.ConclusionThis study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred in Europe and the USA. The study also revealed three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies.
Does a combined intravenous-volatile anesthesia offer advantages compared to an intravenous or volatile anesthesia alone: a systematic review and meta-analysis
Authors: Wolf Alexander , Selpien Helene , Haberl Helge , Unterberg Matthias 
Published: February 2021

Abstract
Background
In anesthesia, additive drug interactions are used for reducing dose and dose-dependent side-effects. The combination of propofol with volatile anesthetics is rather unusual but might have advantages compared to the single use regarding PONV, time to extubation, movement during surgery and postoperative pain perception.

Methods
We searched PubMed, Scopus, Web of Science, and CENTRAL for relevant studies comparing combined intravenous volatile anesthesia with total intravenous or balanced anesthesia. The studies identified were summarized in a meta-analysis with the standardized mean difference or risk ratio as the effect size.

Results
Ten studies provided data. The risk for PONV in the recovery room was significantly reduced for a combined anesthesia compared to a balanced anesthesia (RR 0.657, CI 0.502–0.860, p-value 0.002). There was no significant difference detected either in the time to extubation or in pain perception. Movement during surgery was significantly reduced for a combined compared to a total intravenous anesthesia (RR 0.241, CI 0.135–0.428, p-value 0.000).

Conclusions
The combination of propofol and volatiles may have some advantages in the early occurrence of PONV compared to a balanced anesthesia. To sufficiently evaluate potential advantages of a combination of volatiles and propofol further high-quality trials are needed.

Trial registration
PROSPERO CRD42019126627.
Meta-analysis of metabolic syndrome and its individual components with risk of atrial fibrillation in different populations
Authors: Zheng Ying , Xie Zengshuo , Li Jiayong , Chen Chen , Cai Wenting , Dong Yugang , Xue Ruicong , Liu Chen 
Published: February 2021

AbstractBackgroundRecent studies have reported the effects of metabolic syndrome (MetS) and its components on atrial fibrillation (AF), but the results remain controversial. Therefore, we performed a meta-analysis to evaluate the relationship between MetS and AF risk.MethodsStudies were searched from the Cochrane library, PubMed, and Embase databases through May 2020. Adjusted hazard ratios (HRs) and its corresponding 95% confidence intervals (CIs) were extracted and then pooled by using a random effects model.ResultsA total of 6 observational cohort studies were finally included. In the pooled analysis, MetS was associated with an increased risk of AF (HR 1.57; 95% CI 1.40–1.77;P < 0.01). And the components of MetS including abdominal obesity (HR 1.37; 95% CI 1.36–1.38;P < 0.01), elevated blood pressure (HR 1.56; 95% CI 1.46–1.66;P < 0.01), elevated fasting glucose (HR 1.18; 95% CI 1.15–1.21;P < 0.01) and low high density cholesterol (HDL) (HR 1.18; 95% CI 1.06–1.32;P < 0.01) was also associated with an increased risk of AF, while high triglyceride (HR 0.99; 95% CI 0.87–1.11,P = 0.82) was not.ConclusionsOur present meta-analysis suggested that MetS, as well as its components including abdominal obesity, elevated blood pressure, elevated fasting glucose and low HDL cholesterol were associated with an increase in the risk of AF.
Elevated preoperative neutrophil‐to‐lymphocyte ratio predicts early adverse outcomes in uncomplicated type B aortic dissection undergoing TEVAR
Authors: Zhu Hongqiao , Zhang Lei , Liang Taiping , Li Yiming , Zhou Jian , Jing Zaiping 
Published: February 2021

Abstract
Background
Thoracic aortic endovascular repair (TEVAR) of uncomplicated type B aortic dissection (uTBAD) has favorable long-term outcomes but higher early adverse events compared with the optimal medical treatment. Recently, clinical evidence concerning vascular surgery indicates that elevated preoperative systemic inflammatory response predicts adverse clinical events. The aim of our study was to evaluate the relationship between preoperative neutrophil-to-lymphocyte ratio (NLR) and early outcomes of uTBAD patients undergoing TEVAR.

Results

216 patients diagnosed with uTBAD were included in this retrospective study between January 2015 and December 2018. The median (IQR) follow-up period was 21 (15–33) months. An early adverse event was defined as occurring within 2 years after the procedure. Median patient age was 60 (IQR, 48–68) years and 78.7 % were male. Early adverse events occurred in 24 patients (11.1 %). In the multivariable analysis, preoperative NLR (HR per SD, 1.98; 95 % CI, 1.14–3.44; P = 0.015) was associated with 2-year adverse events.

Conclusions
NLR is an independent predictive factor of early adverse events in uTBAD patients undergoing TEVAR.
Body roundness index is a superior indicator to associate with the cardio‐metabolic risk: evidence from a cross‐sectional study with 17,000 Eastern-China adults
Authors: Xu Jinjian , Zhang Liqun , Wu Qiong , Zhou Yaohan , Jin Ziqi , Li Zhijian , Zhu Yimin 
Published: February 2021

Abstract
Background
To investigate the ability of body shape index (ABSI), body roundness index (BRI), waist circumference (WC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), and body adiposity index (BAI) for predicting non-adipose cardio-metabolic risk.

Methods
A total of 17,360 Chinese subjects aged 18–95 years old who escaped cardiovascular disease (CVD) or diabetes were recruited in the cross-sectional study. Anthropometric and biochemical parameters were assessed. Receiver operating characteristic curve (ROC) and multinomial logistic regression were conducted to examine the association of anthropometric indicators with cardio-metabolic risk factors.

Results
The mean age of subjects were 53.7(13.1) years, 41.6 % were males. The areas under the curve (AUC) demonstrated that WC, BMI, WHR, WHtR and BRI were able to predict high cardio-metabolic risk (AUC > 0.70). Meanwhile, multinomial logistic regression showed BRI was significantly associated with high cardio-metabolic risk (OR 3.27, 95% CI 3.01–3.55). The optimal cut-off values of BRI for high cardio-metabolic risk were (< 60 y: 3.49 vs. ≥60 y: 3.46) in males and (< 60 y: 3.47 vs. ≥60 y: 3.60) in females.

Conclusions
WC, BMI WHR, and WHtR were potential obesity indicators in discriminating high cardio-metabolic risk, while BAI or ABSI was not. Moreover, BRI revealed superior predictive capacity and significant association with accumulated cardio-metabolic risk factors.
Longitudinal association between leisure-time physical activity and vascular elasticity indices
Authors: Szaló Gábor , Hellgren Margareta , Allison Matthew , Råstam Lennart , Lindblad Ulf , Daka Bledar 
Published: February 2021

Abstract
Aim
We investigated the association between levels of leisure-time physical activity and vascular stiffness in a longitudinal observational study from a representative Swedish population.

Method
A total of 2816 randomly selected individuals were examined at visit 1 (2002–2005, Men = 1400). After a mean follow-up of 9.7 ± 1.4 years, a representative sample of 1327 of the original participants were re-examined at visit 2. After excluding subjects with hypertension at baseline, 761 participants were included in the longitudinal analyses. Leisure-time physical (LTPA) activity was self-reported and dichotomized as high or low (level 3, 4 and level 1, 2, respectively). Large Arterial Elasticity Index (LAEI) and Small Arterial Elasticity Index (SAEI) were measured using the HDI/Pulse Wave™ CR2000. Multivariable general linear models were used to investigate the differences in changes SAEI and LAEI based on LTPA levels.

Results
At visit 1, and after adjustment for possible confounders, participants in the high LTPA group had better small artery elasticity (SAEI) (SAEI in low-level LTPA: 7.89 ± 0.11, SAEI in high-level LTPA: 8.32 ± 0.15, ΔSAEI: 0.42, CI: 0.07–0.78; p = 0.020). SAEI decreased between the two assessments (Visit 1: SAEI 8.01 ± 3.37 ml/mmHg; Δ SAEI: 1.4, CI 1.2–1.6, p < 0.001). Participants with a higher LTPA at visit 1 had significantly better SAEI at visit 2 (ΔSAEI: 0.44, CI 0.03–0.85, p = 0.037). No significant associations were observed between LAEI and LTPA after adjustments.

Conclusions
High LTPA predicted higher small arterial compliance at visit 2 suggesting that positive effects of LTPA on arterial elasticity persists over time.
Body size perception, knowledge about obesity and factors associated with lifestyle change among patients, health care professionals and public health experts
Authors: Zelenytė Virginija , Valius Leonas , Domeikienė Auksė , Gudaitytė Rita , Endzinas Žilvinas , Šumskas Linas , Maleckas Almantas 
Published: February 2021

Abstract
Background
The attitudes towards obesity may have an important role on healthier behavior. The goal of the present study was to explore the attitudes towards obesity and to investigate how these attitudes were associated with lifestyle-changing behavior among the patients attending primary care centers, health care professionals and public health experts.

Methods
This cross-sectional survey study was performed in 10 primary care offices in different regions in Lithuania and in 2 public health institutions. Nine hundred thirty-four patients, 97 nurses, 65 physicians and 30 public health experts have filled the questionnaire about attitudes towards obesity and presented data about lifestyle-changing activities during last 12 months. The attitudes were compared between different respondent groups and factors associated with healthier behaviors were analyzed among overweight/obese individuals in our study population.

Results
Participants failed to visually recognize correct figure corresponding to male and female with obesity. Majority of respondents’ perceived obesity as a risk factor for heart diseases and diabetes but had less knowledge about other diseases associated with weight. About one third of respondents changed their lifestyle during last 12 months. Overweight individuals with age < 45 years (OR 1.64, 1.06–2.55; p = 0.025) were more likely and those who overestimated current weight (OR 0.44, 0.20–0.96; p = 0.036) less likely to change their lifestyle. Disappointment with their current weight (OR 2.57, 1.36–4.84; p = 0.003) was associated with healthier behavior among participants with obesity.

Conclusion
Participants had similar body size perception and knowledge about obesity. Younger age had significant association with lifestyle changing behavior among overweight individuals and disappointment with current weight among obese participants.