bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Review of Methods for Long‐Term Electric Load Forecasting
Authors: Aditya Thangjam , Jaipuria Sanjita , Kumar Dadabada Pradeep 
Published: December 2024

ABSTRACTLong‐term load forecasting (LTLF) has been a fundamental least‐cost planning tool for electric utilities. In the past, utilities were monopolies and paid less attention to uncertainty in their LTLF methodologies. Nowadays, such casualness is pricey in competitive markets because utilities need to examine the financial implications of forecast uncertainty for survival. Hence, the aim of this paper is to critique the LTLF research trends with a focus on uncertainty quantification (UQ). For this purpose, we examined 40 LTLF articles published between January 2003 and February 2021. We found that UQ is a nascent area of LTLF research. Our review found two approaches to UQ in LTLF: probabilistic scenario analysis and direct probabilistic methods. The former approach is more helpful to risk analysts but has major caveats in addressing interdependencies of socioeconomic and climate scenarios. We identified very little LTLF research that examines uncertainties associated with climate extremes, distributed generation resources, and demand‐side management. Lastly, there is enormous potential for mitigating financial risks by embracing asymmetric cost functions in LTLF research. Future LTLF researchers can work on these identified gaps to help utilities in risk estimation, cost‐reliability balancing, and estimation of reserve margin under climate change.
Impact of Relative Permeability Hysteresis on CO2 Storage in Saline Aquifers
Authors: Khoramian Reza , Salaudeen Ibraheem , Pourafshary Peyman , Riazi Masoud , Kharrat Riyaz 
Published: December 2024

ABSTRACTThe urgent challenge of climate change, driven by rising carbon emissions, necessitates innovative strategies for carbon capture and storage (CCS). This study examines the impact of hysteresis in relative permeability on CO2 entrapment efficiency within saline aquifers, known for their significant storage capabilities. An aquifer model was analyzed through numerical simulation by varying hysteresis values from 0.2 to 0.5 to evaluate their impact on CO2 plume behavior, retention during water‐alternating‐gas (WAG) injection, and plume morphology. The CO2 plume exhibits a funnel‐shaped configuration at low hysteresis with a narrow, pointed base, indicating a concentrated upward migration trajectory. In contrast, a hysteresis value of 0.5 results in diminished gas movement toward the upper aquifer, transforming the plume into a more oval shape. Results from the land trapping model further support our findings, revealing an inverse relationship where increased hysteresis enhances residual CO2 entrapment, reflected in trapping coefficient values ranging from 0.5 to 4. This underscores the model's efficacy in verifying gas trapping efficiency and safety during sequestration. Moreover, increased water flow generates stronger forces, pushing CO2 into narrower pore spaces, where it becomes trapped. Our findings indicate that increased hysteresis enhances CO2 retention by limiting vertical migration and significantly influences plume geometry, promoting stable and predictable distribution patterns. At higher hysteresis values, CO2 migration is significantly restricted, resulting in near‐complete immobilization of the injected gas. This research highlights hysteresis's critical role in refining injection methodologies and enhancing plume stability for long‐term CO2 storage. © 2024 Society of Chemical Industry and John Wiley & Sons, Ltd.
Postmortem genetic diagnosis of hereditary leiomyomatosis and renal cell carcinoma syndrome: Identification through normal kidney tissues after surgical removal
Authors: Suzuki Shodai , Yamamoto Yoshiyuki , Kato Taigo , Hatano Koji , Matsui Takahiro , Hashimoto Kae , Miyamura Takako , Nagashima Yoji , Nonomura Norio , Kawashima Atsunari 
Published: December 2024

IntroductionHereditary leiomyomatosis and renal cell cancer is an autosomal dominant disorder caused by germline mutations in the FH gene and is associated with poor prognosis of aggressive renal cancer.Case presentationA 33‐year‐old man presented with asymptomatic gross hematuria and was diagnosed with a right renal tumor, cT3aN1M0. He underwent open radical nephrectomy, and pathological examination revealed papillary renal cell carcinoma. Despite aggressive treatment, the disease progressed rapidly, and discussions regarding genetic testing could not take place during his lifetime, although circulating‐tumor DNA showed mutation of FH gene. After death, his wife requested postmortem genetic testing. Genetic analysis using DNA extracted from normal kidney tissues in surgical specimens (blood sample absence) confirmed the FH mutation, and hereditary leiomyomatosis and renal cell cancer was diagnosed posthumously.ConclusionThis highlights the utility of postmortem genetic testing of surgical specimens to diagnose hereditary leiomyomatosis and renal cell cancer and provide genetic counseling to families, despite limitations during the patient's life.
Effectiveness of intermittent cash incentives to increase step counts
Authors: Batchelder Sydney R. , Devoto Amanda , Washington Wendy Donlin 
Published: December 2024

AbstractOnly 25% of adults meet both aerobic and strength training recommendations for physical activity. Contingency management interventions have been used to increase physical activity; however, they may be cost prohibitive. Intermittently provided incentives lower costs and are effective for various health behaviors. The present study investigated whether intermittent cash incentives can increase physical activity (step counts). The researchers used a reversal design with 21 participants, and goals during the intervention were set using a percentile schedule. Contingent on meeting goals, participants could earn the opportunity to draw tickets that corresponded to either no cash or cash incentives. Step counts significantly increased from baseline to the intervention phase. Overall, intermittent cash incentives may be a viable and cost‐effective approach to promoting health behavior.
Construction of Candida albicans pRB895‐SAP2‐SC5314 With SAP2 High Expression and Its Effects on Adhesion
Authors: Xue Lan , Yang Lu , Zhao Bingqian , Feng Wenli , Yang Jing , Ma Yan 
Published: December 2024

ABSTRACTBackgroundSAP2 is closely associated with the pathogenicity and drug resistance of Candida albicans (C. albicans). Our study aimed to construct C. albicans with SAP2 overexpression (pRB895‐SAP2‐SC5314) to explore the influence of SAP2 on the adhesion of C. albicans and predict the interaction between magnolol and Sap2 by molecular docking.MethodsThe recombinant plasmid pRB895‐SAP2 with high SAP2 expression was prepared using a plasmid extraction kit and transformed into C. albicans strain SC5314 using an improved lithium acetate conversion method to construct PRB895‐SAP2‐SC5314. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) was performed to detect the expression of adhesion‐related genes in the different strains. Molecular docking and visual analysis of magnolol and Sap2 were performed using the CB‐DOCK2 platform.ResultsCompared with the control SC5341 and SC5341 transfected with pRB895, SAP2 expression was significantly higher in the pRB895‐SAP2‐SC5314 strain (p < 0.05). Based on the sequencing and mapping results, the pRB895‐SAP2‐SC5314 strain was successfully prepared. SAP2 overexpression significantly downregulated ALS1 expression (p < 0.05), whereas ALS3, TEC1, HOG1, PHR1, and TUP1 expression was downregulated in C. albicans (p < 0.05). The optimal docking result for magnolol and Sap2 protein was −8.1 kcal/mol of vina score, which was considered good docking.ConclusionsSAP2 overexpression may strengthen the adhesion and pathogenicity of C. albicans, and magnolol may act as an Sap2 inhibitor that affects the adhesion of C. albicans.
Production Parameters and Carbon Footprint in Small‐Scale Dairy Farming in Boyacá, Colombia
Authors: Corredor Camargo Emma Sofía , Serna Mendoza Ciro Alfonso , Páez Barón Edwin Manuel 
Published: December 2024

ABSTRACTCattle farming is a significant contributor to greenhouse gas emissions and plays a crucial role in food security, especially in developing countries and in systems that use part of the production for self‐consumption. However, it is not completely clear how production processes impact on the environment. This research aimed to analyse the generation of greenhouse gasses and associated production factors on small‐scale dairy farms in order to identify management strategies to reduce emissions. A quantitative, nonexperimental and correlational study was carried out on 50 farms whose main objective is milk production, located in three municipalities in the central area of the Department of Boyacá. The variables calculated included the percentage of milking females, efficiency per area, per year, per female, and animal load. Greenhouse gas emissions were estimated using life cycle assessment. Data were processed using RStudio, applying the Shapiro–Wilk test, Spearman correlation coefficient, and multiple linear regression analysis for the variables amenable to modeling. The results indicated the total carbon footprint ( 1.8 kg CO2eq/L), EFCF ( 0.06 kg CO2eq/L), MMCF ( 0.21 kg CO2eq/L), and SNFCF ( 1.53 kg CO2eq/L), with SNFCF identified as the major contributor to total CF. The variables of the percentage of cows in milking ( 41.3%) and stocking rate ( 2.37 GLU/ha) exhibited the highest correlation coefficients, particularly with EFCF and SNFCF. The potential for enhancing the environmental sustainability of dairy production through the adoption of efficient management practices concerning manure, soil, and animal components is evident.
In Situ Photochemical Synthesis of Environmentally Friendly Conductive Nano‐Ink
Authors: Konar Melisa , Turgut Him Ebru , Guler Nergis , Balci Ezgi Nur , Arsu Nergis 
Published: December 2024

AbstractAdvancements in nanotechnology allow for the development of in situ or ex situ prepared nanocoatings and nanoparticles which are valuable in the electronics industry due to their intrinsic physical and chemical properties. Printed electronics, intended for applications such as flexible displays and smart labels, offer a distinct advantage over conventional products. The primary components used in printed electronics are the nano‐ink containing polymer matrix and the substrate for printing. Gold, silver, and copper are commonly chosen for these processes because of their excellent electrical conductivity. In the electronics industry, it is well‐known that producing conductive ink typically requires high temperatures and extended processing times, as is the case with conventional methods. In this study, in situ prepared silver nanoparticles (AgNPs) in a crosslinked polymeric matrix are achieved simultaneously using environmentally friendly polyvinyl alcohol (PVA) and acrylamide via photopolymerization. The generated radicals facilitated the formation of silver nano particles while simultaneously forming a cross‐linked network that underwent further processing to transform into ink. The obtained polymer ink formulation is suitable for use with a refillable pen, and it is determined that it dries shortly after writing on surfaces such as paper and glass and exhibits high durability under room conditions.
Rapid Cognitive Deterioration in Progressive Supranuclear Palsy: A 1‐Year Follow‐Up Study
Authors: Li Xin‐Yi , Yang Yu‐Jie , Jiao Fang‐Yang , Tang Gan , Chen Ming‐Jia , Yao Rui‐Xin , Zhao Yi‐Xin , Liang Xiao‐Niu , Shen Bo , Sun Yi‐Min , Wu Jian‐Jun , Wang Jian , Liu Feng‐Tao ,   for the Progressive Supranuclear Palsy Neuroimage Initiative (PSPNI)
Published: December 2024

AbstractBackgroundNowadays, cognitive impairment has been characterized as one of the most vital clinical symptoms in progressive supranuclear palsy (PSP).ObjectivesBased on a relatively large cohort, we aimed to show the cognitive deterioration in different PSP subtypes during 1‐year follow‐up and investigate potential contributors for disease prognosis.MethodsOne hundred seventeen patients from Progressive Supranuclear Palsy Neuroimage Initiative (PSPNI) cohort underwent neuropsychological tests and 1‐year follow‐up, with 73 diagnosed as PSP‐Richardson syndrome (PSP‐RS) and 44 as PSP‐non‐RS. Patients were divided into normal cognition (PSP‐NC), mild cognitive impairment (PSP‐MCI), and PSP‐dementia. Cognitive impairment and progression rates were compared between PSP‐RS and PSP‐non‐RS, and determinants for MCI conversion to dementia were calculated by multiple cox regression.ResultsAt baseline, 30.8% of PSP patients were diagnosed as dementia, 53.0% as MCI, and only 16.2% as NC. Compared to PSP‐non‐RS, PSP‐RS suffered more from motor symptoms and cognitive impairment. During follow‐up, PSP‐RS also exhibited faster disease progression in Mini‐Mental State Examination and visuospatial function, with cognitive deterioration in attention and executive function, but retained in language and memory subdomains. Twenty‐seven of 62 PSP‐MCI patients converted to dementia during follow‐up, with the diagnosis of RS subtype as the most significant contributor to conversion (hazard ration = 2.993, 95% confidence interval = 1.451, 5.232, P = 0.009).ConclusionsPatients with PSP‐RS showed more severe cognitive impairment and faster decline longitudinally than patients with PSP‐non‐RS. Additionally, the diagnosis of RS subtype appears to be the most contributed factor for MCI conversion to dementia within just 1‐year follow‐up period.
A Single‐Layer Wideband Differentially‐Fed Dual‐Polarized Filtenna With Low Cross‐Polarization
Authors: Li Dajiang , Huang Xinzhou , Yang Fan , Xiong De‐Yi , Kang Xin , Hu Kun‐Zhi , Chen Zhiyuan , Yan Dong 
Published: December 2024

ABSTRACTIn this paper, a single‐layer differentially‐Fed dual‐polarized filtenna (DFDPF) with low cross‐polarization is developed. The developed DFDPF consists of four shorted driven patches and four triangular parasitic patches. First, each single‐feed driven patch resonates at its TM10 mode (corresponding to the antiphase TM20 mode of the two differentially‐fed patches). Incorporating shorting pins on the driven patches leads to a lower radiation null. Then, the parasitic patches are embedded into the gap between the driven patches to introduce an extra in‐band resonance operating in its TM1/2,1/2 mode, along with an upper radiation null, while the footprint remains unenlarged. This improves operating bandwidth and roll‐off rate on the upper passband edge. Finally, a pair of symbiotic open‐ended l‐shaped stubs are integrated into each driven patch to further enhance the suppression level of the upper stopband. The developed DFDPF was prototyped and measured for experimental verification. Experimental measurements validate the feasibility of the simulation results, demonstrating a wide –10 dB fractional impedance bandwidth of 19.5% and a peak realized gain of 6.6 dBi. In addition, the cross‐polarization level is lower than –40 dB.
Altered Nigral Amide Proton Transfer Imaging Signal Concordant With Motor Asymmetry in Parkinson's Disease: A Multipool CEST MRI Study
Authors: Tian Yaotian , Li Xinyang , Chen Haibo , Su Wen , Li Shuhua , Zheng Dandan , Li Chunmei , Chen Min 
Published: December 2024

ABSTRACTAsymmetry is a natural characteristic of Parkinson's disease (PD), which can be used to distinguish PD from atypical parkinsonism. Chemical exchange saturation transfer (CEST) has demonstrated value in reflecting the subtle changes related to neuron loss and abnormal protein accumulation in PD but has not been used to investigate asymmetry in PD. This study aimed to examine asymmetrical changes in the mesencephalic nucleus of PD patients with motor asymmetry using four‐pool CEST analysis and to explore the relationship between imaging asymmetry and motor asymmetry. Forty‐six PD cases with motor asymmetry (PD_MA) and 23 normal controls (NC) were included. The patients were divided into three subgroups based on their conditions: PD with mild motor asymmetry (PD_MMA), PD with severe motor asymmetry (PD_SMA), and hemiparkinsonism (PD_Hemi). Differences among the more affected and less affected sides of cases in various PD subgroups and the NC group were analyzed. Motor laterality and imaging laterality were determined to evaluate the concordance. Motor asymmetry and imaging asymmetry indexes were calculated to evaluate the correlation. Compared with NC, amide proton transfer (APT) was significantly decreased in the more affected sides of substantia nigra (SN) and red nucleus in the PD_MA, PD_SMA, and PD_Hemi groups. In the PD‐SMA group, the APT signal in the SN was significantly reduced in the more affected side compared with the less affected side. The imaging asymmetry index in APT for the SN was positively associated with the motor asymmetry index in the PD_SMA group (β = 0.431, p = 0.014). The imaging laterality in APT for the SN had a significant consistency with motor laterality in the PD_SMA group (κ = 0.566, p = 0.001). These findings suggest inherent asymmetry of APT signal in the SN in PD patients with severe asymmetry, with nigral APT potentially serving as a noninvasive biomarker of lateralization in PD.
Nucleic Acid Specificity, Cellular Localization and Reduced Toxicities of Thiazole Orange‐Neomycin Conjugates
Authors: McFarland Antwine W. , Fernando Lawrence P. , Kellish Patrick , Story Sandra P. , Schober Gretchen B. , Kumar Sunil , Gong Changjun , King Ada , Gong Xianchang , Leutou Alain S. , Arya Dev P. 
Published: December 2024

AbstractSelective binding of small molecule ligands to nucleic acids with high affinity and limited toxicity remains an important goal in the development of compounds that can probe DNA or RNA in cells. Thiazole orange is a cell semi‐permeant, fluorescent cyanine dye, with low background noise, that binds several forms of nucleic acids. However, thiazole orange can exhibit cytotoxicity when used at high concentration and/or with prolonged exposure. Neomycin is a non‐fluorescent antibiotic with affinity for several forms of nucleic acids, but does not readily enter mammalian cells. Conjugation of neomycin with thiazole orange can exploit the properties of each individual compound, yielding a small molecule that could be used for nontoxic application in cellular analysis by microscopic imaging. We demonstrate that conjugation of neomycin with thiazole orange increases the cell permeability of neomycin, decreases the cytotoxicity of thiazole orange, and exhibits a greater degree of intracellular RNA targeted localization in the nucleolus, when compared to thiazole orange. Relative to thiazole orange, the conjugated compounds showed a much higher degree of stabilization of the nucleic acids as reflected in a greater denaturation temperature. Ultimately, our studies indicate that the conjugated thiazole orange‐neomycin compounds can be used as an RNA targeted, less cytotoxic alternative for cellular labeling.
Similar but Distinct—Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH and FphI
Authors: Fellner Matthias , Randall George , Bitac Ianah R. C. G. , Warrender Annmaree K. , Sethi Ashish , Jelinek Raz , Kass Itamar 
Published: December 2024

ABSTRACTStaphylococcus aureus is a major cause of infections like bacteremia, pneumonia, and endocarditis. These infections are often linked to the ability of S. aureus to form biofilms. Several S. aureus serine hydrolases have previously been identified to be active during biofilm‐forming conditions. Here, we present the biochemical characterization of two of these enzymes—fluorophosphonate binding hydrolase H and I (FphH, FphI). Cryogenic and room‐temperature X‐ray crystallography, enzymatic substrate profiling, small‐angle X‐ray scattering analysis, and molecular dynamics simulations provide new insights into similarities and differences between these two hydrolase_4 domain family members. We discover that these enzymes share an overall fold, including a flexible lid or cap region above the active site, which can be seen to be mobile in solution. Differences in the active site pocket and lid residues differentiate them and explain speed differences in their carboxyesterase substrate profile toward small unbranched carbon chain ester molecules. The first analysis of FphI is also compared to our previous knowledge of FphH and its association to stress conditions. These results enable the future precise targeting of Fph serine hydrolase family members with a long‐term goal to significantly improve the health and wellbeing of individuals and populations worldwide.
Bacillus endophytes for sustainable management of tomato spotted wilt virus and yield production
Authors: Khan Rizwan , He Pengbo , Chen Xiaojiao , He Pengfei , Ahmed Ayesha , Wu Yixin , Tang Guowen , Tang Ping , Li Xingyu , Munir Shahzad , He Yueqiu 
Published: December 2024

AbstractBACKGROUNDTomato‐spotted wilt virus (TSWV) from the Tospovirus genus affects over 1000 plant species, including key crops, and traditional control methods often prove inadequate. This study investigates the effectiveness of Bacillus amyloliquefaciens and Bacillus subtilis in reducing TSWV infection, enhancing plant growth, and strengthening defense in Nicotiana benthamiana. The aim is to assess Bacillus as a sustainable biocontrol alternative, offering an eco‐friendly solution for managing TSWV disease in agriculture.RESULTSHere, we report the efficacy of five Bacillus isolates (out of 15 tested) – B. amyloliquefaciens (DJB5, YN48, YN28, Mg6) and B. subtilis L1‐21 – significantly reducing TSWV copies per gram in N. benthamiana leaves, using a half‐leaf assay. In glasshouse trials, isolates DJB5, YN48, and Mg6 decreased TSWV copies per gram by 75.7%, 83.6%, and 88.2%, with biocontrol efficacy rates of 91.2%, 94.1%, and 95.7% respectively. All the isolates consistently mitigated the symptoms of TSWV, reduced the disease severity, and area under the disease progress curve (AUDPC) at 21 days post‐inoculation. Additionally, these isolates enhanced plant growth parameters, including shoot and root length, leaf number, area, and biomass. The application of endophytes in the infected plants activated antioxidant defense enzymes by elevating the activities of polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), and chitinase. However, defense‐related enzymes, such as malondialdehyde (MDA), catalase (CAT), phenylalanine ammonia‐lyase (PAL), total phenol, and β‐1,3‐glucanase decreased as TSWV infection reduced in the leaves.CONCLUSIONOur findings indicate that B. amyloliquefaciens isolates, DJB5, YN48, and Mg6, effectively manage TSWV by activating plant defense, reducing virus load, reducing TSWV symptoms, and promoting plant growth. © 2024 Society of Chemical Industry.