bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lung Function In Discharged Patients After Coronavirus Infection (SARS, MERS, COVID-19): A Systemic Review And Meta-Analysis
Authors: Cui Yan Li , Cheng Wen-Wen , Mou Zhi-Wei , Xiao Duan , Shan Sha-Rui , Chen Zhuo-Ming 
Published: May 2021

Abstract
Background: To date, coronaviruses have caused three pandemics. Fewer studies concentrated on the prognosis of lung function.Objective: To summarize the lung function of the discharged after coronavirus infection.Methods: We systematically searched PubMed, Cochrane Library, Web of Science, and EMBASE. Two authors independently screened articles and extracted data. On average, predicted values and damage rates of seven lung function indices were pooled by single-arm meta-analysis. And, in severe/critical vs. non-severe/critical and one-year follow-up, they were pooled by two-arm meta-analysis. The source of high heterogeneity was explored by meta-regression or subgroup analysis. Results: Of the 7798 articles identified, 34 studies were included. On average, the pooled predicted values of the seven indices were within normal except for DLCO (79.2, 95% CI (76.2－82.2 )). Damage of lung function indices accounted for 6.2－35.2% of the discharged with DLCO most, and 83－100% of the damage was mild. Meta-regression showed that different viruses, countries, disease settings, and measurement times were not the source of high heterogeneities. In severe/critical illness vs. non-severe/critical, predicted values of seven indices were significantly lower (largest gap in DLCO (WMD -11.60, 95% CI -14.23－-8.98)). However, damage rates got rises only in DLCO (RR 1.74, 95% CI 1.46－2.07) and TLC, having no differences in the other indices. In one-year follow-up, predicted values were significantly improved in the severe/critical subgroup, while having no change in the non-severe/critical subgroup. Damage rates got no improvement in all indices.Interpretation: A single predicted value or damage rate can't give a clear description of lung function after coronavirus infection, and the trends of the two are sometimes inconsistent. We suggest more prospective cohort or follow-up studies in the future to lessen the influence of differences in lung function measurements across studies.Registration: PROSPERO (CRD42020192843)
Exogenous Application of Sodium Hydrosulfide Suppresses Bacterial Wilt And Regulates The Soil Microecology
Authors: Li Chunli , Yu Jun , Li Zongping , Yang Jinpeng , Chen Shouwen , Yang Yong , Yang Chunlei , Hu Yun 
Published: May 2021

Abstract
The role of hydrogen sulfide (H2S) in regulating the pathogenic bacteria has been well documented. However, whether exogenous H2S addition inhibits the pathogens in soil is not understood, and whether H2S can suppress the plant disease caused by pathogen R. Solanacearum is not clear. In the present study, different concentrations of H2S donor NaHS were applied to the tobacco field to explore the interrelation among NaHS, tobacco baterial wilt, soil physicochemical properties and microbial community. In order to decipher the disease suppression mechanism from the perspective of soil microecology. Application of NaHS significantly reduced the disease incidence and disease index of TBW, increased soil pH, alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), available phosphorus (AP) and organic matter (OM). NaHS addition also changed soil microbial community composition and structure. Furthermore, NaHS addition significantly reduced the abundance of Ralstonia and Fusarium, and increas pathogenic ed beneficial microorganisms Solirubrobacter, Rhodococcus, Rhizobium, Pseudomonas, Paenibacillus, Microvirga, Lysobacter, Haliangium, Granulicella, Flavobacterium, Bacillus, Trichoderma and Aspergillus at the genus level. Our findings suggested that exogenous application of NaHS significantly suppressed TBW caused by R. Solanacearum through regulated soil microecology. This study revealed the potential of NaHS in control of bacterial wilt.
Identification of differentially expressed genes and hub genes of human hosts with tuberculosis through an integrated bioinformatics strategy
Authors: Yue Peng , Dong Yan , Xu Xin , Zhang Yu , Kong Jing , Chen Jingjing , Fan Yuxin , Liu Meixiao , He Yuan , Cao Wenjing , Wen Shiyuan , Li Binxue , Luo Lisha , Chen Taigui , Li Lianbao , Wang Feng , Zhou Guozhong , Luo Suyi , Liu Aihua , Bao Fukai 
Published: May 2021

Abstract
Background: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Until now, molecular mechanisms underlying the occurrence, development and prognosis of tuberculosis have not been fully characterized. The aim of the study was to identify hub genes involved in tuberculosis.Methods: We used four microarray datasets (GSE51029, GSE52819, GSE54992, and GSE65517) from the Gene Expression Omnibus (GEO) and GEO2R software to identify differentially expressed genes (DEGs) between samples from humans infected with M. tuberculosis and a healthy control group (using cutoffs of LogFC > 1 and p value < 0.05). DEGs shared by the four microarray datasets were further identified. Next, we carried out functional enrichment analysis using the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG); Then, the host hub genes with a relatively high number of connections to other DEGs, were identified by Cytoscape. Other bioinformatics methods are also performed, including protein–protein interaction (PPI) network analysis and construction of miRNA–hub gene networks and transcription factors (TF)–hub gene networks. Finally, the expression of the host hub genes was verified using the reverse transcription polymerase chain reaction(RT–PCR).Results: A total of 46 DEGs were identified. GO analysis showed that the biological functions of DEGs were mainly in immune response regulation, cytokine/chemokine activity, and receptor ligand activity. DEGs were significantly enriched in membrane rafts, the mitochondrial outer membrane, cytoplasmic vesicle cavities, and nuclear chromatin. KEGG enrichment analysis showed involvement of the genes in the NOD-like receptor and toll-like receptor signaling pathways. Five highly differentially expressed hub genes – STAT1, TLR7, CXCL8, CCR2, and CCL20 – were identified. Finally, based on NetworkAnalyst's database, we constructed miRNA–hub gene networks and TF–hub gene networks.Conclusions: In summary, bioinformatics analyses were used to identify DEGs to find potential biomarkers that may be associated with tuberculosis. This study provides a set of candidate DEGs and five important hub genes that can be potential for the early detection, prognostic determination, risk assessment, and targeted therapy of tuberculosis.
A Scale to Assess Factors Influencing Treatment Initiation in Patients With Anorexia Nervosa. Development and Evaluation of the FABIANA-Checklist.
Authors: Reuter Laurence , Kästner Denise , Schmidt Justine , Weigel Angelika , Voderholzer Ulrich , Seidel Marion , Schwennen Bianca , Fehrs Helge , Löwe Bernd , Gumz Antje 
Published: May 2021

Abstract
BackgroundA long duration of untreated illness (DUI) is an unfavorable prognostic factor in anorexia nervosa and often associated with chronic illness progression. The FABIANA- checklist, developed using a qualitative multi-informant approach, includes factors influencing treatment initiation which are potentially modifiable and are mentioned as being relevant by patients with AN. The study focusses on the development and evaluation of the FABIANA-Checklist and aims at providing descriptive data on DUI in a German sample.MethodsAfter cognitive pretest and revision, dimensionality of the 18-item version of the FABIANA-checklist was tested by Principal Component Analysis (PCA). For item validation we assessed support from social environment (FSozU), support in the health care system (PACIC-5A), illness perception and coping (BIPQ).ResultsWe included a sample of female patients (N=75), aged >14 years ( M = 21.4 years) with AN. Average BMI was 15.5 kg/m 2 , age of onset was 19.2 years and average DUI was 2.25 years. PCA yielded six components explaining 62.64% of the total variance. Overall internal consistency was acceptable (Cronbach’s α= .76) and construct validity was satisfactory for 14 items. Two consistent components emerged: primary care perceived as supportive and competent (23.33%) and emotional and practical support from relatives (9.98%). With regard to the other components, the heterogeneity of the items led to unsatisfactory internal consistency, single item loading and partly ambiguous interpretability. Analysis on item level indicated that practical and emotional support from the social environment seems to be particularly important for treatment initiation.ConclusionsThe FABIANA-checklist is a valid instrument to assess factors involved in the process of treatment initiation of patients with AN. Psychometrics and dimensionality testing suggests that experienced emotional and practical support from the primary health care system and close relatives are strongly involved factors. Nevertheless, the results point out that a differentiated consideration on item level is useful. To quantify the relative importance of the factors assessed and to derive recommendations on early-intervention approaches, the predictive effect of the FABIANA-items on the DUI will be determined in a subsequent study which will further include the perspective of relatives and primary caregivers in addition to the patient perspective.Trial registrationClinical Trials.gov Identifier: NCT03713541: https://clinicaltrials.gov/ct2/show/NCT03713541
Improved structure and enhanced the insecticidal activity of Sip1Aa protein by adding disulfide bonds
Authors: Wang Lin , Wang Jing , Ding Ming-Yue , Gao Ji-Guo , Liu Rong-mei , Li Hai-Tao 
Published: May 2021

Abstract
Sip1Aa is an insecticidal protein of Bacillus thuringiensis at the secretory stage. It has a strong toxic effect on the members of order Coleoptera. To date, there are few available studies on Sip1Aa protein and the inclusion body problem is serious, and this raises the importance to conduct further studies on Sip1Aa protein. Disulfide bonds, as the only covalent bond on protein side chains, play an important role in the stability and function of the proteins. The tertiary structure of Sip1Aa protein was analyzed by homologous modeling and other bioinformatics methods to predict the conserved domain of Sip1Aa protein. Cysteine used to replace these amino acids by site-directed mutation. Consequently, we were able to successfully construct Sip149-251, Sip153-248, Sip158-243, and Sip178-314. These were exposed to ultraviolet radiation, and we found that Sip153-248 and Sip158-243 were the most stable, followed by Sip149-251 and Sip178-314 when compared with Sip1Aa. After the mutant strain was transferred into Escherichia coli BL21, sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to detect the inducible expression products. Approximately 37.6 kDa of proteins that were highly expressed in E. coli. We found no significant change in the insecticidal activity.
Bulging Distortion of Austenitic Stainless Steel Sheet on the Partially Penetrated Side of Non-Penetration Lap Laser Welding Joint
Authors: Yao Chengwu , Liu Enze , Ni Jiaming , Nie Binying 
Published: May 2021

Abstract
Non-penetration laser welding of lap joints in austenitic stainless steel sheets is commonly preferred in fields where the surface quality is of utmost importance. However, the application of non-penetration welded austenitic stainless steel parts is limited owing to the micro bulging distortion that occurs on the back surface of the partial penetration side. In this study, non-penetration lap laser welding experiments, were conducted on galvanized and SUS304 austenitic stainless steel plates using a fiber laser, to investigate the mechanism of bulging distortion. A comparative experiment of DC01 galvanized steel-Q235 carbon steel lap laser welding was carried out, and the deflection and distortion profile of partially penetrated side of the sheets were measured using a non-contact laser interferometer. In addition, the cold-rolled SUS304 was subjected to heat holding at different temperatures and water quenching after bending to characterize its microstructure under tensile and compressive stress. The results show that, during the heating stage of the thermal cycle of laser lap welding, the partial penetration side of the SUS304 steel sheet generates compressive stress, which extrudes the material in the heat-affected zone to the outside of the back of the SUS304 steel sheet, thereby forming a bulge. The findings of these experiments can be of great value for controlling the distortion of the partial penetrated side of austenitic stainless steel sheet during laser non-penetration lap welding.
Scientific Collaboration Network Analysis: A Brazilian Computer Science Graduate Programs Case
Authors: Costa Aurélio Ribeiro , Nunes Vanessa Tavares , Ralha Célia Ghedini 
Published: May 2021

Abstract
Scientific collaboration networks can present different views of researchers’ interaction. The analysis of these networks allows research transparency focusing on the understanding of how science evolves in different contexts. In this article, we present a scientific collaboration network analysis using social network metrics as average degree, connected components, betweenness and closeness centralities applied to five Brazilian Computer Science graduate programs. An online artifact built upon the design science research paradigm, namely SCI-synergy, was developed to ease the analysis involving publication data available on the Digital Bibliography & Library Project (DBLP) from the graduate program members allied to the Federal University of Minas Gerais (UFMG), the State University of São Paulo (USP), the Federal University of Rio Grande do Norte (UFRN), the Federal University of Amazonas (UFAM), and the University of Brasília (UnB). The objective is to analyze the scientific collaboration network of each program focusing on the researchers’ collaboration to understand patterns considering intra- and inter-program relationships. The analysis covers two database updates of 2019 and 2021. Results show that intra-program relationships are not always bigger among the highest-ranked programs in CAPES. Nevertheless, UFMG presents a greater total of cooperation among the five programs indicating research leadership and good inter-program collaboration patterns. We advocate that the collaboration network analysis using an online artifact can be useful to understand patterns of all the Brazilian Computer Science graduate programs to discover new research perspectives to be improved, avoided or even applied to other contexts.
Identification of Whole Blood C Ircular RNA Expression Profiles and Potential Regulatory Roles in Children with Human A denovirus Pneumonia
Authors: Yang Diyuan , Sun Ke , Huang Feng , Fan Huifeng , Shi Tingting , Chen Xinxin , Lu Gen 
Published: May 2021

Abstract
Human adenoviruses (HAdVs) causes infections predominantly in infants and young children, and severe HAdVs pneumonia results in high lethality rate in children. CircRNAs are novel identified long non-coding RNAs and play important roles in gene regulation and pathogenesis of disease. To elucidate the roles of circRNAs in HAdVs pneumonia, we analyzed the circRNAs profiles of healthy children and children with HAdVs pneumonia including mild and severe cases, and identified 139 significant upregulated circRNAs in children with HAdVs pneumonia vs healthy controls, and 18 significant upregulated circRNAs in children with severe HAdVs pneumonia vs mild HAdVs pneumonia. In particular, hsa_circ_0002171 was found differently expressed in both groups, which may be taken as diagnostic biomarkers for HAdVs pneumonia and severe HAdVs pneumonia. To identify the underlying mechanisms of circRNAs in HAdVs pneumonia, we analyzed the transcriptome of children with HAdVs pneumonia and established circRNA-mRNA regulatory network. Enrichment of the differentially expressed (DE) target mRNAs demonstrated that DE genes between healthy controls vs HAdVs pneumonia were mainly involved in RNA splicing, while DE genes between children with mild and severe HAdVs pneumonia were mainly from the regulation of lymphocyte activation. Receiver operating characteristic (ROC) curve analysis suggested that hsa_circ_0002171 had significant value in HAdVs pneumonia and severe cases diagnosis. Taken together, profile of circRNA expression was altered in children with HAdVs pneumonia and severe cases. These results demonstrate that hsa_circ_0002171 as a potential diagnostic biomarker of HAdVs pneumonia and severe cases and may be a novel therapeutic target.
Effects of uric acid, creatinine clearance, and infusion duration on the population pharmacokinetics of meropenem in critically ill patients
Authors: Zhao Yi Chang , Zou Yang , Xiao Yi Wen , Wang Feng , Zhang Bi Kui , Xiang Da Xiong , Yu Feng , Luo Hong , Yan Miao 
Published: May 2021

Abstract
Background
Meropenem is a carbapenem antibiotic that has demonstrated excellent in vitro activity against gram-negative clinical Isolates and is commonly used in critically ill patients. This study aimed to describe the population pharmacokinetics (PPK) of meropenem and determine the optimal dosage in these patients.
Method:
We included 209 samples in 64 patients in this prospective study. PPK analysis and Monte Carlo dosing simulations were developed using Phoenix.
Results
A two-compartment model described the data adequately. Clearance (CL), volume (V), clearance of peripheral compartment (CL2), volume of peripheral compartment (V2) were 6.15 L/h, 2.83 L/h, 17.40L, and 17.48L, respectively. Creatinine clearance and uric acid were significant covariates. Patients with creatinine clearance of 60 ml/min or less and uric acid greater than 400 µmol/l could achieve the target >\(\)90% under the minimum inhibitory concentration (MIC) of 8 mg/L, even with the administration dose of 500 mg/8 h with a 2-h infusion. Prolonging the infusion time significantly improved the therapeutic effect when MIC༜4. However, for the pharmacodynamic (PD) effects of 100% fT > MIC and 100% fT > 4MIC, no significant statistical difference was observed in critically ill patients.
Conclusions
Critically ill patients with lower creatinine clearance and higher uric acid levels were likely to need a lower dosage of meropenem. Prolonged infusion time were not appear to be beneficial for those who need a higher therapeutic target (100% fT > MIC,100% fT > 4 MIC) or with MIC\(>\)4mg/L. Increasing dose or alternative therapeutic strategies may be required for critically ill patients with drug-resistant or severe infections. The study is of great significance to guide the rational use of meropenem in critically ill patients.
Trial registration:
The trial was registered in the China Clinical Trial (ChiCTR1900020672). Registered on 12 January 2019.
T-cell responses to SARS CoV-2 in unexposed South African women
Authors: nunes marta , Johnson Michael , Kwatra Gaurav , Weinberg Adriana , Madhi Shabir 
Published: May 2021

Abstract
A potential explanation for the fact that the high force of infection for SARS-CoV-2 in South Africa did not translate into high rates of severe illness and death, may be the presence of cross-reactive immunity induced by common cold coronaviruses (CCoV). We used SARS-CoV-2 peptide pools and whole virus antigen to stimulate peripheral blood mononuclear cells collected pre-2020 from South African women and measured interferon gamma (IFNγ) and interleukin 2 (IL2) production. IFNγ responses were observed in 29.9% of the women and IL2 among 39.2%. Overall, 51.6% of women demonstrated response to at least one stimulant. The responses to SARS-CoV-2 might have been induced by past exposure to CCoV.
Video-Assisted Thoracoscopic Lobectomy Versus Open Lobectomy In The Treatment Of Large Lung Cancer: Propensity-Score Matched Analysis
Authors: Yun Jeonghee , Lee Junghee , Shin Sumin , Kim Hong Kwan , Choi Yong Soo , Kim Jhingook , Zo Jae Il , Shim Young Mog , Cho Jong Ho 
Published: May 2021

Abstract
Background: There are several concerns on thoracoscopic surgery for large tumors because of the increased risk of tumor cell spillage. This study aimed to compare perioperative outcomes and oncological validity between video-assisted thoracoscopic surgery (VATS) and open lobectomy for non-small cell lung cancer (NSCLC) with tumor size >5cm.Methods: We retrospectively reviewed 355 patients who underwent lobectomy with clinical N0 NSCLC with solid tumor component diameter > 5cm between January 2009 and December 2016. Patients with tumor invading adjacent structures were excluded. The patients were divided into the VATS group (n = 132) and thoracotomy group (n = 223). Propensity score matching (1:1) was applied.Results: After propensity score matching, 204 patients were matched, and clinical characteristics of the two groups were well balanced. The VATS group was associated with a shorter length of hospital stay (6 days vs. 7 days; P < 0.001) than the thoracotomy group. There were no significant differences in the 5-year overall survival (71.5% in VATS vs. 64.4% in thoracotomy, P = 0.390) and 5-year recurrence-free survival (60.1% in VATS vs. 51.5% in thoracotomy, P = 0.210) between the two groups. The cumulative incidence of ipsilateral pleural recurrence was not significantly different between the two groups (12.0% in VATS vs. 7.9% in thoracotomy; P = 0.582).Conclusions: In clinical N0 NSCLC larger than 5cm, VATS lobectomy resulted in shorter hospital stay and similar survival outcome compared to open lobectomy. Based on these results, VATS lobectomy is a valuable option in this subset of patients.
Linear Late Gadolinium Enhancement In The Basal Anterior Septum And Lateral Wall May Represent The Anterior Septal Perforator Artery And Circumflex Artery, Respectively: A CMR And CCTA Comparison Study
Authors: Ma Peisong , Shang Yongnning , Hu Yurou , Liu Juan , Zhou Xiaoyue , Wang Jian 
Published: May 2021

Abstract
Purpose: The purpose of this paper was to verify that the linear high-intensity signal on late gadolinium enhancement-cardiac magnetic resonance (LGE-CMR) may represent the contrast enhancement of vessels rather than scars or fibrosis, and to assess whether this linear high-intensity signal will affect the quantification of myocardial fibrosis in patients with hypertrophic cardiomyopathy (HCM).Methods: A total of 58 patients who underwent both coronary computed tomography angiography (CCTA) and LGE-CMR in our hospital were ultimately enrolled. The definitions of positive linear LGE (LLGE+) were as follows: (1) LLGE in the basal anterior septum or lateral wall, and (2) LLGE observable at 10 mm or more. All other patients were regarded as negative LLGE (LLGE-). In LLGE+ patients, the length of the LLGE located in the anterior septum and lateral wall was compared with the length of the septal perforator artery and the circumflex artery on CCTA, respectively. For nine patients with HCM, the LGE% was measured before and after removal of LLGE.Results: Among the 58 patients, 40 of whom showed LLGE+ and 18 of whom showed LLGE-. For patients with LLGE in the anterior septum, there was a strong correlation between LLGE and anterior septal perforator arteries in length (r=0.887, p<0.001). For patients with LLGE in the lateral wall, LLGE also correlated well with the circumflex arteries in length (r=0.962, p<0.001). In nine patients with HCM, the LGE% decreased significantly after the removal of LLGE (18.6 [14.15 - 27.5] vs. 12.4 [9.8 - 17.5], p<0.05).Conclusions: The LLGE in the anterior septum and lateral wall may represent contrast enhancement of the anterior septal perforator artery and the circumflex artery, respectively. This LLGE may overestimate the extent of myocardial fibrosis in patients with hypertrophic cardiomyopathy.