bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geological and Geomorphological Characteristics of High-Priority Landing Sites for the Luna-Glob Mission
Authors: Krasilnikov S. S. , Basilevsky A. T. , Ivanov M. A. , Krasilnikov A. S. 
Published: April 2021

Abstract
The geomorphological and geological analysis of the surface of three primary and ten secondary landing ellipses (15 × 30 km) for the Russian Luna-Glob automated scientific station (Luna-25) is presented. The earlier selected landing ellipses are found in the sector of 65°–85° S and 0°–60° E of the lunar southern hemisphere. The geological characteristics of the ellipse include the analysis of the surface age and the identification of the material sources. The analyses of water equivalent hydrogen concentration in the sector of landing and rock content on the surface are also carried out. The constraints, such as the surface inclination and the characteristic of the spacecraft’s flight path are used for prioritization of the ellipses from an engineering-technical perspective. According to the results of the study, landing ellipses 1, 3, 4, 6 and 8 are determined as high-priority, while ellipses 5, 7, 9, 10, 11, as well as B1 and B2 are found the least favorable.
Predicting lying, sitting, walking and running using Apple Watch and Fitbit data
Authors: Fuller Daniel , Anaraki Javad Rahimipour , Simango Bongai , Rayner Machel , Dorani Faramarz , Bozorgi Arastoo , Luan Hui , A Basset Fabien 
Published: April 2021

ObjectivesThis study’s objective was to examine whether commercial wearable devices could accurately predict lying, sitting and varying intensities of walking and running.MethodsWe recruited a convenience sample of 49 participants (23 men and 26 women) to wear three devices, an Apple Watch Series 2, a Fitbit Charge HR2 and iPhone 6S. Participants completed a 65 min protocol consisting of 40 min of total treadmill time and 25 min of sitting or lying time. The study’s outcome variables were six movement types: lying, sitting, walking self-paced and walking/running at 3 metabolic equivalents of task (METs), 5 METs and 7 METs. All analyses were conducted at the minute level with heart rate, steps, distance and calories from Apple Watch and Fitbit. These included three different machine learning models: support vector machines, Random Forest and Rotation forest.ResultsOur dataset included 3656 and 2608 min of Apple Watch and Fitbit data, respectively. Rotation Forest models had the highest classification accuracies for Apple Watch at 82.6%, and Random Forest models had the highest accuracy for Fitbit at 90.8%. Classification accuracies for Apple Watch data ranged from 72.6% for sitting to 89.0% for 7 METs. For Fitbit, accuracies varied between 86.2% for sitting to 92.6% for 7 METs.ConclusionThis preliminary study demonstrated that data from commercial wearable devices could predict movement types with reasonable accuracy. More research is needed, but these methods are a proof of concept for movement type classification at the population level using commercial wearable device data.
Incidence of emergency calls and out-of-hospital cardiac arrest deaths during the COVID-19 pandemic: findings from a cross-sectional study in a UK ambulance service
Authors: Charlton Karl , Limmer Matthew , Moore Hayley 
Published: April 2021

BackgroundIn response to the COVID-19 pandemic, a national lockdown was introduced on 23 March 2020. In the following weeks, emergency departments in the UK reported a reduction in attendances. We aimed to explore the incidence of emergency calls across North East England, as well as the number of out-of-hospital cardiac arrest (OHCA) deaths.MethodsData were collected for all patients who contacted North East Ambulance Service between 4 March 2019–2 June 2019 and 2 March 2020–31 May 2020 suffering stroke, ST elevation myocardial infarction, allergy, asthma, chronic obstructive pulmonary disease, falls, intoxication, seizure, sepsis, acute coronary syndrome and OHCA.ResultsThere were a reduction in incidence of calls, excluding OHCA, resulting in ambulance activation during the pandemic compared with same period in 2019, 16 743 versus 19 639, respectively (−14.74%). The decline in calls was partially reversed by the end of May 2020. Incidence of OHCA at the time of the national lockdown had increased by 13.79% with a peak increase of 73.56% in the second week in April 2020. OHCA deaths peaked in the first 2 weeks in April 2020, 95.65% and 90.07%, respectively, but by the end May 2020, incidence of OHCA and OHCA deaths had returned to prelockdown levels.ConclusionIncidence of emergency calls were reduced during the pandemic compared with 2019. There was a rise in incidence of OHCA and OHCA deaths during the same period; however, these changes appear transient. Further research is required to understand patient behaviour towards seeking help during the pandemic and the long-term consequences of not doing so.
Psychological distress during the acceleration phase of the COVID-19 pandemic: a survey of doctors practising in emergency medicine, anaesthesia and intensive care medicine in the UK and Ireland
Authors: Roberts Tom , Daniels Jo , Hulme William , Hirst Robert , Horner Daniel , Lyttle Mark D , Samuel Katie , Graham Blair , Reynard Charlie , Barrett Michael , Foley James , Cronin John , Umana Etimbuk , Vinagre Joao , Carlton Edward 
Published: April 2021

ObjectiveTo quantify psychological distress experienced by emergency, anaesthetic and intensive care doctors during the acceleration phase of COVID-19 in the UK and Ireland.MethodsInitial cross-sectional electronic survey distributed during acceleration phase of the first pandemic wave of COVID-19 in the UK and Ireland (UK: 18 March 2020–26 March 2020 and Ireland: 25 March 2020–2 April 2020). Surveys were distributed via established specialty research networks, within a three-part longitudinal study. Participants were doctors working in emergency, anaesthetic and intensive medicine during the first pandemic wave of COVID-19 in acute hospitals across the UK and Ireland. Primary outcome measures were the General Health Questionnaire-12 (GHQ-12). Additional questions examined personal and professional characteristics, experiences of COVID-19 to date, risk to self and others and self-reported perceptions of health and well-being.Results5440 responses were obtained, 54.3% (n=2955) from emergency medicine and 36.9% (n=2005) from anaesthetics. All levels of doctor seniority were represented. For the primary outcome of GHQ-12 score, 44.2% (n=2405) of respondents scored >3, meeting the criteria for psychological distress. 57.3% (n=3045) had never previously provided clinical care during an infectious disease outbreak but over half of respondents felt somewhat prepared (48.6%, n=2653) or very prepared (7.6%, n=416) to provide clinical care to patients with COVID-19. However, 81.1% (n=4414) either agreed (31.1%, n=2709) or strongly agreed (31.1%, n=1705) that their personal health was at risk due to their clinical role.ConclusionsFindings indicate that during the acceleration phase of the COVID-19 pandemic, almost half of frontline doctors working in acute care reported psychological distress as measured by the GHQ-12. Findings from this study should inform strategies to optimise preparedness and explore modifiable factors associated with increased psychological distress in the short and long term.Trial registration numberISRCTN10666798.
Shed antigen-induced blocking effect on CAR-T cells targeting Glypican-3 in Hepatocellular Carcinoma
Authors: Sun Luan , Gao Fang , Gao Zhanhui , Ao Lei , Li Na , Ma Sujuan , Jia Meng , Li Nan , Lu Peihua , Sun Beicheng , Ho Mitchell , Jia Shaochang , Ding Tong , Gao Wei 
Published: April 2021

BackgroundGlypican-3 (GPC3), a cell surface glycoprotein that is pathologically highly expressed in hepatocellular carcinoma (HCC), is an attractive target for immunotherapies, including chimeric antigen receptor (CAR) T cells. The serum GPC3 is frequently elevated in HCC patients due to the shedding effect of cell surface GPC3. The shed GPC3 (sGPC3) is reported to block the function of cell-surface GPC3 as a negative regulator. Therefore, it would be worth investigating the potential influence of antigen shedding in anti-GPC3 CAR-T therapy for HCC.MethodsIn this study, we constructed two types of CAR-T cells targeting distinct epitopes of GPC3 to examine how sGPC3 influences the activation and cytotoxicity of CAR-T cells in vitro and in vivo by introducing sGPC3 positive patient serum or recombinant sGPC3 proteins into HCC cells or by using sGPC3-overexpressing HCC cell lines.ResultsBoth humanized YP7 CAR-T cells and 32A9 CAR-T cells showed GPC3-specific antitumor functions in vitro and in vivo. The existence of sGPC3 significantly inhibited the release of cytokines and the cytotoxicity of anti-GPC3 CAR-T cells in vitro. In animal models, mice carrying Hep3B xenograft tumors expressing sGPC3 exhibited a worse response to the treatment with CAR-T cells under both a low and high tumor burden. sGPC3 bound to CAR-T cells but failed to induce the effective activation of CAR-T cells. Therefore, sGPC3 acted as dominant negative regulators when competed with cell surface GPC3 to bind anti-GPC3 CAR-T cells, leading to an inhibitory effect on CAR-T cells in HCC.ConclusionsWe provide a proof-of-concept study demonstrating that GPC3 shedding might cause worse response to CAR-T cell treatment by competing with cell surface GPC3 for CAR-T cell binding, which revealed a new mechanism of tumor immune escape in HCC, providing a novel biomarker for patient enrolment in future clinical trials and/or treatments with GPC3-targeted CAR-T cells.
Tobacco mythbusting—tobacco is not a major driver of foot traffic in low socio-economic small retail stores
Authors: Wood Lisa , Gazey Angela 
Published: April 2021

BackgroundOne of the opposing arguments to restricting or banning the sale of tobacco products stem from a perception that this would adversely impact on small retail stores that rely on tobacco sales for viability. It has also been argued that purchases of tobacco leads to unplanned purchasing of other items that yield income for small store owners. This study tested the veracity of these arguments in the Australian context.MethodsConsumer intercept surveys (n=1487) were conducted outside a comprehensive sample of small stores (n=136) selling tobacco in lower socioeconomic suburbs. Data were collected over a 2-hour period outside each store using the same methodology (36% consumer response rate). Descriptive statistics examined the proportion of tobacco and non-tobacco purchases and most common products purchased.ResultsPurchasing tobacco was the primary motivation for store visits for only 3% of consumers. The vast majority of products purchased (92%) were not tobacco, with hot food, groceries and lottery tickets most frequent. Only 8% of consumers purchased tobacco. When unplanned purchasing patterns were compared, consumers’ who purchased tobacco were no more likely to buy other products.ConclusionTobacco purchasing was rarely the reason for store visits, indicating that it is not a key driver of consumer foot traffic for small retailers. There was also no evidence that tobacco contributes to spontaneous purchases of other products that might bring retailers profit. Findings suggest that restricting the retail availability of tobacco would be unlikely to have a pronounced negative impact on small retail stores.
Illicit tobacco trade: empty pack survey in eight Argentinean cities
Authors: Pizarro Maria Elisabet , Giacobone Gabriel , Shammah Cinthia , Stoklosa Michal 
Published: April 2021

ObjectiveTo estimate the prevalence of illicit tobacco trade (ITT) and different ITT modes—tax stamp counterfeiting and smuggling—in Argentina.DesignCross-sectional study using an empty tobacco pack survey with a simple random cluster sampling design. Classification as licit/illicit using forensic analysis of tax stamps and packs and econometric modelling.SettingCities of Buenos Aires, La Matanza, Cordoba, Rosario, Mendoza, Neuquen, Posadas, Salta; January–June 2019.ResultsOf a total sample of 15 658 packs, 83.2% were manufactured in Argentina and 16.8% were foreign packs. Overall ITT prevalence—weighted by district population size—was estimated at 13.7%, where 6.1% was attributable to stamp counterfeiting—that is, a forged stamp not issued by the national tax authority—and 7.6% to contraband smuggling of foreign cigarette packs—that is, illicit trade of packs across national borders.ConclusionsThe ITT problem in Argentina seems to be equally represented by counterfeiting of tobacco tax stamps on packs with domestic features and smuggling of foreign cigarette packs. Foreign cigarettes represent a minor component of the pack sampled in most of the country, except in Salta and Posadas, which are located close to the border with Paraguay. It is essential to implement an effective track-and-trace system including the monitoring of tax stamp authenticity and increase border control to block the entry of smuggled products, particularly from Paraguay. Reducing ITT is necessary to ensure the effectiveness of tobacco taxation measures.
Secondary disturbance following a deposit of volcanic tephra: a 30-year record from old-growth forest understory
Authors: Zobel Donald B. , Antos Joseph A. , Fischer Dylan G. 
Published: April 2021

Forest disturbance is usually described by effects on trees, and small disturbances to forest understory are seldom studied. Nevertheless, effective analyses of succession need to consider both stand-replacing and subsequent “secondary” disturbances in both the canopy and understory. We estimated the characteristics of 13 types of secondary disturbances in old-growth forest understory, as well as change in the canopy cover, after the deposition of tephra (aerially transported volcanic ejecta) in 1980 from Mount St. Helens, Washington. We sampled 100 plots (1 m2) at each of four sites for vegetation change and types of disturbance at 10 different times from 1980 to 2010; we sampled the tree canopy above each plot in 1980 and 2016. The number of canopy gaps increased by 23% and mean gap dimension increased by 68% over 36 years, mostly from the loss of Abies amabilis (Douglas ex Loudon) Douglas ex Forbes. Secondary disturbance in understory affected 1.4% of stand area per year. The areas affected by soil disturbance and effects of woody litter were similar. Erosion, greater in deep than in shallow tephra, peaked in 1981, whereas most litter-caused disturbances increased after 2000. Less frequent litter-based disturbances covered greater area. Our results differ from conclusions about nonvolcanic understory disturbances. Secondary disturbances are variable, need more study, and are likely to affect many other systems.
Fertilizing with composted cattle manure to meet agro-environmental targets in continuous corn and corn–soybean agroecosystems in southern Quebec
Authors: Elmi Abdirashid , Jiao You , Benslim Hicham , Mawof Ali , Prasher Shiv O. , Whalen Joann K. 
Published: April 2021

Balancing nutrient inputs from manure and supplemental inorganic fertilizers with crop requirements should achieve crop yield goals and agro-environmental targets. We asked if composted dairy cattle manure, alone or in combination with inorganic fertilizers, could sustain the corn and soybean yields, without increasing the residual soil nitrogen (N) and soil phosphorus (P) saturation of a sandy-loam soil in southern Quebec, Canada. Cropping systems were continuous silage corn and corn–soybean rotation, with each phase of the rotation grown every year, for 5 yr. Cropping systems were amended with compost at rates of 0, 15, 30, and 45 Mg (wet weight)·ha−1, and they received supplemental inorganic fertilizer to supply 200 kg N·ha−1·yr−1 to corn while assuring that corn and soybean received 45 kg P·ha−1·yr−1 and 125 kg potassium (K)·ha−1·yr−1 from compost and inorganic fertilizer. Crop yields were similar in compost-amended and inorganically fertilized plots. Corn yield was limited by N availability, and there was no P or K limitation in corn and soybean. Soil P saturation in the 0–15 cm layer exceeded the agro-environmental limit of 13.1% P/Al for a sandy-loam soil in Quebec, indicating that the P input exceeded crop uptake. Residual soil N concentration was affected more by the crop than the fertilizer source, with greater residual soil N in plots with higher grain production. Crop-induced priming can explain how N fertilizer cycles through the soil microbial community and is gradually mineralized during the growing season, and why crop senescence may trigger residual soil N release after harvest.
Multi-user Remote Lab: Timetable Scheduling Using Simplex Nondominated Sorting Genetic Algorithm
Authors: Zandavi Seid Miad , Chung Vera , Anaissi Ali 
Published: April 2021

The scheduling of multi-user remote laboratories is modeled as a multimodal function for the proposed optimization algorithm. The hybrid optimization algorithm, hybridization of the Nelder-Mead Simplex algorithm, and Non-dominated Sorting Genetic Algorithm (NSGA), named Simplex Non-dominated Sorting Genetic Algorithm (SNSGA), is proposed to optimize the timetable problem for the remote laboratories to coordinate shared access. The proposed algorithm utilizes the Simplex algorithm in terms of exploration and NSGA for sorting local optimum points with consideration of potential areas. SNSGA is applied to difficult nonlinear continuous multimodal functions, and its performance is compared with hybrid Simplex Particle Swarm Optimization, Simplex Genetic Algorithm, and other heuristic algorithms. The results show that SNSGA has a competitive performance to address timetable problems.
Plasma KIM-1 Is Associated with Recurrence Risk after Nephrectomy for Localized Renal Cell Carcinoma: A Trial of the ECOG-ACRIN Research Group (E2805)
Authors: Xu Wenxin , Puligandla Mäneka , Halbert Brian , Haas Naomi B. , Flaherty Keith T. , Uzzo Robert G. , Dutcher Janice P. , DiPaola Robert S. , Sabbisetti Venkata , Bhatt Rupal S. 
Published: April 2021

Abstract

Purpose:
No circulating biomarkers are currently available to identify patients at highest risk of recurrence after nephrectomy for renal cell carcinoma (RCC). Kidney injury molecule-1 (KIM-1) is overexpressed in RCC and its ectodomain circulates in plasma. We investigated whether plasma KIM-1 is a prognostic biomarker in patients with localized RCC after nephrectomy.

Experimental Design:
The ECOG-ACRIN E2805 (ASSURE) trial evaluated adjuvant sunitinib, sorafenib, or placebo in resected high-risk RCC. KIM-1 levels were measured from banked plasma at trial enrollment 4–12 weeks after nephrectomy. Lognormal accelerated failure time models were used to test for association between KIM-1 and disease-free survival (DFS) as well as overall survival (OS).

Results:
Plasma from 418 patients was analyzed. Higher post-nephrectomy KIM-1 was associated with worse DFS across all study arms after adjustment for Fuhrman grade, T stage, N stage, and tumor histology [survival time ratio 0.56 for 75th vs. 25th percentile of KIM-1; 95% confidence interval (CI), 0.42–0.73; P &lt; 0.001]. The association between KIM-1 and DFS was stronger among patients with pathologic nodal involvement (Pinteraction = 0.0086). The addition of post-nephrectomy KIM-1 improved the concordance of clinical prognostic models [Stage, Size, Grade, and Necrosis (SSIGN) concordance 0.57 vs. 0.43, P = 0.05; UCLA International Staging System (UISS) concordance 0.60 vs. 0.40, P = 0.0005]. Higher post-nephrectomy KIM-1 was also associated with worse OS after multivariable adjustment (survival time ratio 0.71 for 75th vs. 25th percentile of KIM-1; 95% CI, 0.56–0.91; P &lt; 0.001).

Conclusions:
Post-nephrectomy plasma KIM-1 is associated with DFS and OS in RCC, and may be a biomarker for microscopic residual disease.
Análise de modelos de maturidade para medição da implementação do Building Information Modeling (BIM)
Authors: Lima Luciana de Oliveira , Catai Rodrigo Eduardo , Scheer Sergio 
Published: April 2021

O Building Information Modeling (BIM) é considerado uma inovação disruptiva, pois modifica profundamente as soluções técnicas de uma organização. A implementação desta inovação, no entanto, é repleta de desafios. Avaliar o desenvolvimento da implementação BIM pela medição da maturidade pode auxiliar as organizações, que passam a conhecer as próprias competências e a identificar os próximos passos evolutivos. Porém, diante de diversos modelos de maturidade desenvolvidos internacionalmente, não há estudos que comprovem a aplicabilidade dessas ferramentas no contexto brasileiro. Desta forma, a presente pesquisa objetiva analisar três modelos - BIM Assessment Profile, BIM Maturity Matrix e VDC Scorecard - para disseminar o uso dessas ferramentas. Procedimentos comparativos e de estudos de campo foram os métodos de pesquisa utilizados. Os modelos foram examinados com base na análise das métricas e dos princípios básicos, aplicados em nove organizações da AEC de Curitiba-PR, distinguidas as particularidades de aplicação, e por fim, foram apontadas as características de qualidade de cada um. Os resultados mostram que todos apresentam questões processuais, técnicas e de pessoas. O BIM Assessment Profile apresenta qualidades básicas, simples e flexíveis, adequado a organizações que estão em estágios iniciais de adoção do BIM. O BIM Maturity Matrix, também de caráter básico, simples e flexível, é indicado para organizações pequenas ou médias. Já o VDC Scorecard, o mais complexo, apresenta características básicas, descritivas e prescritivas, e apesar de ser o menos flexível, é o mais abrangente. As aplicações práticas geraram resultados ao identificar o grau de maturidade BIM das empresas e projetos avaliados.
Altered Responsiveness to TGFβ and BMP and Increased CD45+ Cell Presence in Mitral Valves Are Unique Features of Ischemic Mitral Regurgitation
Authors: Castillero Estibaliz , Howsmon Daniel P. , Rego Bruno V. , Keeney Samuel J. , Driesbaugh Kathryn H. , Kawashima Takayuki , Xue Yingfei , Camillo Chiara , George Isaac , Gorman Robert C. , Gorman Joseph H. , Sacks Michael S. , Levy Robert J. , Ferrari Giovanni 
Published: April 2021

Objective:
Ischemic mitral regurgitation (IMR) often develops after an ischemic event, which results in distortion of the valvulo-ventricular complex and incomplete mitral valve (MV) leaflet coaptation. After left ventricular ischemic events, only some patients develop IMR. The susceptibility of the MV to remodel may influence whether IMR develops. We hypothesized that impaired signaling response in MV cells may contribute to IMR development by inducing maladaptive tissue remodeling.

Approach and Results:
Sheep (n=14) were subjected to ligation of the circumflex coronary artery to induce myocardial infarction. IMR was reported by echocardiography. MV leaflets and MV interstitial cells (MVICs) were collected at baseline (control, n=10), 4 and 8 weeks post-myocardial infarction. RNA sequencing highlighted differences in TGFβ (transforming growth factor beta) signaling between MV with/without IMR. SMAD6/7 and ID2 (inhibitor of DNA binding 2) were the highest increased TGFβ-signaling genes associated with IMR. MVICs from myocardial infarction sheep were less responsive to BMP (bone morphogenic protein) 4 pro-osteogenic stimulation (ID2, OPN [osteopontin], and OC [osteocalcin] mRNA) than control. MVICs from IMR sheep had a diminished COL (collagen) 1A1 mRNA response to TGFβ1 and enhanced prochondrogenic RUNX2 (runt-related transcription factor 2) and SOX9 mRNA response to BMP4 versus non-IMR MVICs. Baseline CD45 (cluster of differentiation) expression was detectable only in IMR MVICs. Upon TGFβ1 stimulation, CD45 expression was detected in all groups. Immunostaining confirmed increased presence of CD45+ cells in IMR MV interstitium.

Conclusions:
MVs from sheep with IMR had an altered TGFβ/BMP response, associated with increased CD45+ cell presence within the tissue interstitium. Pharmacological strategies aimed to modulate TGFβ/BMP signaling after myocardial infarction may protect from pathological MV remodeling leading to IMR.