bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Binding of the N- and C-terminal Cytoplasmic Domains of Aquaporin 4 to Calmodulin May Contribute to Vesicular Trafficking
Authors: Ishida Hiroaki , Vogel Hans J. , Conner Alex C. , Kitchen Philip , Bill Roslyn M. , MacDonald Justin A. 
Published: June 2021

ABSTRACTAquaporin 4 (AQP4) is a water transporting, transmembrane channel protein that has important regulatory roles in maintaining cellular water homeostasis. Several other AQP proteins exhibit calmodulin (CaM)-binding properties, and CaM has recently been implicated in the cell surface localization of AQP4 that occurs in response to osmotically-driven changes in cell swelling in the central nervous system. The objective of the present study was to assess the CaM-binding properties of AQP4 in detail. Inspection of AQP4 revealed two putative CaM-binding domains (CBDs) in the cytoplasmic N- and C-terminal regions, respectively. The Ca2+-dependent CaM-binding properties of synthetic and recombinant AQP4 CBD peptides were assessed using fluorescence spectroscopy, isothermal titration calorimetry, and two-dimensional 1H, 15N-HSQC NMR with 15N-labeled CaM. The N-terminal CBD peptide of AQP4 predominantly interacted with the N-lobe of CaM with a 1:1 binding ratio and a Kd of 3.4 μM. CaM bound two C-terminal AQP4 peptides with interactions observed for both the C- and N-lobes of CaM (Kd1: 3.6 μM, Kd2: 113.6 μM, respectively). A recombinant AQP4 protein domain (rAQP4ct, containing the entire cytosolic C-terminal domain sequence) bound CaM in a 1:1 binding mode with a Kd of 6.1 μM. A ternary bridging complex could be generated with the N- and C-lobes of CaM interacting simultaneously with the N- and C-terminal CBD peptides. These data suggest that this unique adapter protein binding mode of CaM and AQP4 may be an important regulatory mechanism for the vesicular trafficking of AQP4.
Synthesis and characterization of 5-(2-fluoro-4-[11C]methoxyphenyl)-2,2-dimethyl-3,4-dihydro-2H-pyrano[2,3-b]pyridine-7-carboxamide as a PET imaging ligand for metabotropic glutamate receptor 2
Authors: Yuan Gengyang , Dhaynaut Maeva , Lan Yu , Guehl Nicolas J. , Huynh Dalena , Iyengar Suhasini , Afshar Sepideh , Wang Hao , Moon Sung-Hyun , Ondrechen Mary Jo , Wang Changning , Shoup Timothy , El Fakhri Georges , Normandin Marc D. , Brownell Anna-Liisa 
Published: June 2021

AbstractMetabotropic glutamate receptor 2 (mGluR2) is a therapeutic target for the treatment of several neuropsychiatric disorders and conditions. The role of mGluR2 function in etiology could be unveiled byin vivoimaging using positron emission tomography (PET). In this regard, 5-(2- fluoro-4-[11C]methoxyphenyl)-2,2-dimethyl-3,4-dihydro-2H-pyrano[2,3-b]pyridine-7- carboxamide ([11C]13), a potent negative allosteric modulator (NAM), was developed to support this endeavor. Radioligand [11C]13was synthesized via theO-[11C]methylation of phenol24with a high molar activity of 212 ± 76 GBq/µmol (n = 5) and excellent radiochemical purity (> 99%). PET imaging of [11C]13in rats demonstrated its superior brain heterogeneity, particularly in the regions of striatum, thalamus, hippocampus, and cortex. Accumulation of [11C]13in these regions of interest (ROIs) was reduced with pretreatment of mGluR2 NAMs, VU6001966 (9) and MNI-137 (26), the extent of which revealed a time-dependent drug effect of the blocking agents. In a nonhuman primate, [11C]13selectively accumulated in mGluR2-rich regions, especially in different cortical areas, putamen, thalamus, and hippocampus, and resulted in high-contrast brain images. The regional total volume of distribution (VT) estimates of [11C]13decreased by 14% after the pretreatment with9. Therefore, [11C]13is a potential candidate for translational PET imaging studies of mGluR2 function.
Prefrontal cortical connectivity mediates locus coeruleus noradrenergic regulation of inhibitory control in older adults
Authors: Tomassini Alessandro , Hezemans Frank H. , Ye Rong , Cam-CAN  , Tsvetanov Kamen A. , Wolpe Noham , Rowe James B. 
Published: June 2021

AbstractResponse inhibition is a core executive function enabling adaptive behaviour in dynamic environments. Human and animal models indicate that inhibitory control and control networks are modulated by noradrenaline, arising from the locus coeruleus. The integrity (i.e., cellular density) of the locus coeruleus noradrenergic system can be estimated from magnetization transfer sensitive magnetic resonance imaging, in view of neuromelanin present in noradrenergic neurons of older adults. Noradrenergic psychopharmacological studies indicate noradrenergic modulation of prefrontal and frontostriatal stopping-circuits in association with behavioural change. Here we test the noradrenergic hypothesis of inhibitory control, in healthy adults. We predicted that locus coeruleus integrity is associated with age-adjusted variance in response inhibition, mediated by changes in connectivity between frontal inhibitory control regions. In a preregistered analysis, we used magnetization transfer MRI images from N=63 healthy adults aged above 50 years who performed a stop-signal task, with atlas-based measurement of locus coeruleus contrast. We confirm that better response inhibition is correlated with locus coeruleus integrity and stronger connectivity between pre-supplementary motor area and right inferior frontal gyrus, but not volumes of the cortical regions. We confirmed a significant role of prefrontal connectivity in mediating the effect of individual differences in the locus coeruleus on behaviour, whereby this effect was moderated by age, over and above adjustment for the mean effects of age. Our results support the hypothesis that in normal populations, as in clinical settings, the locus coeruleus noradrenergic system regulates inhibitory control.
Glycomic profiling of the gut microbiota by Glycan-seq
Authors: Oinam Lalhaba , Minoshima Fumi , Tateno Hiroaki 
Published: June 2021

AbstractBackgroundThere has been immense interest in studying the relationship between the gut microbiota and human health. Bacterial glycans modulate the cross talk between the gut microbiota and its host. However, little is known about these glycans because of the lack of appropriate technology to study them.MethodsWe previously developed a sequencing-based glycan profiling method called Glycan-seq, which is based on the use of 39 DNA-barcoded lectins. In this study, we applied this technology to analyze the glycome of the intact gut microbiota of mice. Fecal microbiota was incubated with 39 DNA-barcoded lectins exposed to UV, and the number of released DNA barcodes were counted by next-generation sequencing to obtain a signal for each lectin bound to the microbiota. In parallel, the bacterial composition of the gut microbiota was analyzed by 16S rRNA gene sequencing. Finally, we performed a lectin pull-down experiment followed by 16S rRNA gene sequencing to identify lectin-reactive bacteria.ResultsThe evaluation of cultured gram-positive (Deinococcus radiodurans) and gram-negative (Escherichia coli) bacteria showed significantly distinct glycan profiles between these bacteria, which were selected and further analyzed by flow cytometry. The results of flow cytometry agreed well with those obtained by Glycan-seq, indicating that Glycan-seq can be used for bacterial glycomic analysis. We thus applied Glycan-seq to comparatively analyze the glycomes of young and old mice gut microbiotas. The glycomes of the young and old microbiotas had significantly distinct glycan profiles, which reflect the different bacterial compositions of young and old gut microbiotas based on 16S rRNA gene sequencing. Therefore, the difference in the glycomic profiles between young and old microbiotas may be due to their differing bacterial compositions. α2-6Sia-binders bound specifically to the young microbiota. Lectin pull-down followed by 16S rRNA gene sequencing of the young microbiota identified Lactobacillaceae as the most abundant bacterial family with glycans reacting with α2-6Sia-binders.ConclusionThe Glycan-seq system can, without any prior culturing and fluorescence labeling, reveal the glycomic profile of the intact bacterial gut microbiota. A combination of lectin pull-down and 16S rRNA gene sequencing can identify lectin-reactive bacteria.
A systematic review of adverse childhood experiences (ACEs) with people with intellectual disabilities: an unsafe gap in the literature
Authors: Crompton Chloe , Duncan Bethany , Simpson-Adkins Graham 
Published: June 2021

Purpose
This paper aims to systematically review the available evidence that explores adverse childhood experiences (ACEs) in people with intellectual disabilities (PwID). It is important to systematically review this literature as, to date, there is little known about the number of studies in this area, despite the World Health Organization declaring ACE prevention and support as a global public health priority.

Design/methodology/approach
Published studies were identified from electronic database searches. Key journals and reference lists were also hand searched.

Findings
Two studies met the inclusion criteria and the prevalence and frequency of ACEs experienced by participants of these studies analysed. Overall, due to the small number of studies meeting the inclusion criteria, it is difficult to establish any meaningful conclusions.

Originality/value
This appears to be the first systematic review to try and identify a research base looking at the prevalence of ACEs within a PwID population. Findings suggest that this is a highly neglected area of research, and the authors hope to have identified that further evidence is required to draw clearer conclusions about the impact of ACEs on PwID.
Business strategies responding to COVID-19: experience of Chinese corporations
Authors: Wu Minyu , Kong Kun 
Published: June 2021

PurposeThis paper aims to investigate the corporate social responsibility (CSR) initiatives adopted by Chinese firms during the outbreak of COVID-19. Facing this unknown, unexpected and devastating disease, Chinese corporations demonstrated their CSR in different approaches. The purpose of this paper is to explore how CSR influences the decisions of the corporations that respond to a severe incident and how corporations can achieve their mission or strategic objectives by responding to a serious incident.Design/methodology/approachBased on secondary data and thematic analysis, this paper examines six Chinese corporations which are the leading firms in their respective industries.FindingsThis paper finds that firms adopted a mixed approach to conducting their CSR initiatives, including altruistic, strategic and citizenship CSR initiatives. This paper also confirms that strategic CSR initiatives were in line with the five dimensions of strategic CSR including centrality, specificity, proactivity, visibility and voluntarism. In addition, this paper also shows that a company could create its competitive advantage by carrying out CSR initiatives that are able to strengthen its value chain activities or the competitive context. This is based on the partnership built by the firms with their stakeholders to recognize the shared value.Practical implicationsThis paper shows the implication that business leaders should understood the role of a business in society and the importance of stakeholders’ expectations. The underlying philosophy is that CSR could strengthen the resilience of society; business organizations need to operate in a healthy society.Originality/valueThis paper provides insights of Chinese corporations responding to a severe social incident. It highlights the strategic perspective of CSR initiatives and the linkage between CSR activities and a firm’s competitive advantage.
Selection of wearable sensor measurements for monitoring and managing entry-level construction worker fatigue: a logistic regression approach
Authors: Lee Wonil , Lin Ken-Yu , Johnson Peter W. , Seto Edmund Y.W. 
Published: June 2021

PurposeThe identification of fatigue status and early intervention to mitigate fatigue can reduce the risk of workplace injuries. Off-the-shelf wearable sensors capable of assessing multiple parameters are available. However, using numerous variables in the fatigue prediction model can elicit data issues. This study aimed at identifying the most relevant variables for measuring occupational fatigue among entry-level construction workers by using common wearable sensor technologies, such as electrocardiogram and actigraphy sensors.Design/methodology/approachTwenty-two individuals were assigned different task workloads in repeated sessions. Stepwise logistic regression was used to identify the most parsimonious fatigue prediction model. Heart rate variability measurements, standard deviation of NN intervals and power in the low-frequency range (LF) were considered for fatigue prediction. Fast Fourier transform and autoregressive (AR) analysis were employed as frequency domain analysis methods.FindingsThe log-transformed LF obtained using AR analysis is preferred for daily fatigue management, whereas the standard deviation of normal-to-normal NN is useful in weekly fatigue management.Research limitations/implicationsThis study was conducted with entry-level construction workers who are involved in manual material handling activities. The findings of this study are applicable to this group.Originality/valueThis is the first study to investigate all major measures obtainable through electrocardiogram and actigraphy among current mainstream wearables for monitoring occupational fatigue in the construction industry. It contributes knowledge on the use of wearable technology for managing occupational fatigue among entry-level construction workers engaged in material handling activities.
Association of child survival with birth size and mother’s BMI: a human right approach
Authors: Singh Abhishek , Jain Kshipra 
Published: June 2021

PurposeChildren are the most vulnerable group owing to long lasting impact of the violation of human rights in term of proper nutrition and their right to live. The purpose of this paper is to assess the risk of child mortality associated with size of child at birth and mother’s anemia level in northern India.Design/methodology/approachThe data were used from 2015–2016 National Family Health Survey (NFHS). The participants (n= 41,412) were children aged under-five years from north Indian state of Uttar Pradesh. The main outcome measure was child deaths defined by under-five mortality. The univariate, bivariate and multivariate analyses were used in data analysis. Kaplan–Meier analyses, Log-rank tests and Cox’s regression analyses were performed to fulfill the objective of the study.FindingsThere were a total of 2,835 deaths out of 41,412 births in the past five years preceding the survey period. Children of very small size at birth were significantly two and half times more likely to die than children of average size at birth. The estimated adjusted hazard ratio indicated that the children of severely anemic mothers were significantly 1.5 times more likely to die compared to children of not anemic mothers. Size of child at birth, mother’s anemia level, mother’s age at time of her first birth, wealth index and mother’s education were significantly associated with the under-five mortality in northern India.Research limitations/implicationsThe limitation of this study is recall errors arising from the dates of birth and death given by women interviewed in the survey were minimized by restricting the analyses to births within the five-year period preceding the survey.Practical implicationsThis study advocates the promotion of comprehensive prevention strategies through appropriate institutional mechanism would be the best intervention or adaptive mechanism to reduce the adverse impact of size of child at birth, mother’s anemia level on under-five mortality in Uttar Pradesh, India.Originality/valueThis research is original. This study enjoys a unique importance by exploring effect of size of child at birth and mother’s anemia on child survival in developing countries like India.
Role of personal anxiety in individual kaizen behaviour and performance: evidence from Japan
Authors: Yokozawa Kodo , Nguyen Hao Anh , Tran Thi Bich Hanh 
Published: June 2021

PurposeThis study examines the role of anxiety in kaizen behaviour and performance by empirically testing the influence of personal anxiety (state and trait) on individual kaizen behaviours (rule adherence, initiative and perseverance of effort), which, in turn, affect individual kaizen performance.Design/methodology/approachThe data were obtained from a survey of 552 employees of four companies in Japan and analysed using structural equation modelling.FindingsThe results show that state anxiety has a significantly positive effect on rule adherence and kaizen performance. Trait anxiety positively influences employees' initiative and perseverance but has a significant negative effect on kaizen performance.Originality/valueThis study contributes to kaizen and continuous improvement theory by focussing on individual kaizen, which is considered to be as important as organisation-level kaizen and investigating the relevance of personal anxiety in individual kaizen behaviours and kaizen performance.
Digital information product development: lessons from a small-sized German enterprise
Authors: Keller Pascal , Lima Afonso 
Published: June 2021

PurposeThis case study examines the new product development (NPD) process of digital information products (DIPs) and its critical success factors (CSFs) in a small-sized German enterprise.Design/methodology/approachA case study was conducted with a small firm focused on the development of DIPs; data were obtained through semi-structured interviews, direct observation and document analysis.FindingsThe firm uses an informal and cross-functional NPD process (idea generation, idea validation, product creation and product launch) in converting an idea into a sellable product. Peculiar aspects of each stage within the process as well as ten CSFs to NPD projects were verified.Research limitations/implicationsLimitations are attributed to its qualitative nature, which does not allow generalizations, though careful attention was given to validity and reliability issues through the use of data source triangulation.Practical implicationsThe paper provides a conceptual process that may benefit further initiatives for developing and launching DIPs, as well as a comprehensive list of CSFs for such projects.Originality/valueThis paper is the first one to schematize the NPD process and practices for DIPs, as well as key capabilities. It contributes to the NPD literature in discussing dynamic aspects that are typical to the firm analyzed and to others operating in a digital context. At the same time, it reinforces other traditional aspects that have become overlooked in digital business debates.
Modelling the predictors of consumers’ willingness to pay premium price for sustainable products
Authors: Kamboj Shampy , Matharu Manita 
Published: June 2021

PurposeThe purpose of this paper is to increase existing knowledge of consumers’ sustainable products via converging and testing social identity and value-belief-norm theory used in sustainable behaviour literature. More specifically, the present paper incorporated consumers’ orientation towards a lifestyle of health and sustainability (COLOHAS), attitude towards sustainable products’ (ATSP’) purchase, perceived consumer effectiveness and perceived knowledge about sustainability issues as a conceptual framework to understand comprehensively consumers’ willingness to pay premium price (WPPP) for sustainable products.Design/methodology/approachThe data were gathered through a survey of 850 customers residing at Delhi-NCR and buying sustainable products. Data were analysed using the structural equation modelling approach.FindingsThe results show that perceived consumer effectiveness has a greater descriptive value on their WPPP than do their ATSP’s purchase and perceived knowledge about sustainability issues. Additionally, both dimensions (health consciousness and value orientation towards sustainability) of COLOHAS have been found to have a significant and positive influence on consumer ATSP.Research limitations/implicationsThe empirical results offer managers with better insights on how COLOHAS, perceived effectiveness and perceived knowledge about sustainability issues influences their WPPP for sustainable products.Originality/valueThis study adds to the body of literature by emphasizing those cognitive processes that influence the customers’ WPPP for sustainable products. Therefore, this paper presents important information to the managers and marketers about the sustainability aspects.
Investigation and analysis of digital poverty among adolescents in China
Authors: Yuan Jing , Guo Lingyu 
Published: June 2021

PurposeThe purpose of this paper is to investigate the status quo of digital poverty among adolescents in China, analyze the characteristics and the causes, then propose countermeasures to provide reference for alleviating digital poverty among adolescents.Design/methodology/approachThe study developed an initial scale of digital poverty among adolescents and used survey data to revise the scale, on this basis, formed a questionnaire, which was distributed to nationwide adolescents. The study developed its findings from the 837 valid questionnaire respondents.FindingsThe digital poverty among adolescents is mainly shown in the poverty of digital ability, digital psychology and digital environment and presents the following characteristics, that is, insufficient information seeking ability and information selection ability needing to be improved; equipped with basic information awareness but lack of information evaluation ability; lack of patience in obtaining information and inclined to the principle of least effort; imperfect knowledge structure and immature psychological emotions and vulnerable to external interference; having a certain relationship with the information environment, but not significantly affected by regional economic differences. Finally, the study puts forward countermeasures to alleviate digital poverty among adolescents.Practical implicationsUnderstanding of the digital poverty among adolescents will likely demand rethinking into a number of issues ignored by information poverty studies.Originality/valueFew studies focus on digital poverty among adolescents. This study developed an initial scale of digital poverty among adolescents and revised it by survey data, then conducted an empirical study through questionnaire, which could expand the understanding of information poverty in the field of library and information science.