bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct roles of haspin in stem cell division and male gametogenesis
Authors: Soupsana Katerina , Karanika Eleftheria , Kiosse Fani , Christogianni Anastasia , Sfikas Yiorgos , Topalis Pantelis , Batistatou Anna , Kanaki Zoi , Klinakis Apostolos , Politou Anastasia S. , Georgatos Spyros 
Published: October 2021

AbstractThe kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex at the centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing cells, haspin-null mice do not exhibit developmental anomalies, apart from aberrant testis architecture. Investigating this problem, we show here that mouse embryonic stem cells that lack or overexpress haspin, albeit prone to chromosome misalignment during metaphase, can still divide, expand and differentiate. RNA sequencing reveals that haspin dosage affects severely the expression levels of several genes that are involved in male gametogenesis. Consistent with a role in testis-specific expression, H3T3ph is detected not only in mitotic spermatogonia and meiotic spermatocytes, but also in non-dividing cells, such as haploid spermatids. Similarly to somatic cells, the mark is erased in the end of meiotic divisions, but re-installed during spermatid maturation, subsequent to methylation of histone H3 at lysine-4 (H3K4me3) and arginine-8 (H3R8me2). These serial modifications are particularly enriched in chromatin domains containing histone H3 trimethylated at lysine-27 (H3K27me3), but devoid of histone H3 trimethylated at lysine-9 (H3K9me3). The unique spatio-temporal pattern of histone H3 modifications implicates haspin in the epigenetic control of spermiogenesis.
First-in-class topical therapeutic omilancor ameliorates disease severity and inflammation through activation of LANCL2 pathway in psoriasis
Authors: Tubau-Juni Nuria , Hontecillas Raquel , Leber Andrew , Maturavongsadit Panita , Chauhan Jyoti , Bassaganya-Riera Josep 
Published: October 2021

AbstractPsoriasis (PsO) is a complex immune-mediated disease that afflicts 100 million people. Omilancor is a locally-acting, small molecule that selectively activates the Lanthionine Synthetase C-like 2 (LANCL2) pathway, resulting in immunoregulatory effects at the intersection of immunity and metabolism. Topical omilancor treatment in an imiquimod-induced mouse model of PsO ameliorates disease severity, epidermal hyperplasia and acanthosis. Further, pharmacological activation of LANCL2 results in significant downregulation of proinflammatory markers including local reduction of IL17, and infiltration of proinflammatory cell subsets. These therapeutic effects were further validated in an IL-23 PsO model. This model reported increased preservation of homeostatic skin structure, accompanied by a decreased infiltration of proinflammatory T cell subsets. In CD4+ T cells and Th17 cells, the LANCL2 pathway regulates proinflammatory cytokine production, proliferation and glucose metabolism. Metabolically, the loss of Lancl2 resulted in increased glycolytic rates, lactate production and upregulated enzymatic activity of hexokinase and lactate dehydrogenase (LDH). Inhibition of LDH activity abrogated the increased proliferation rate in Lancl2−/− CD4+ T cells. Additionally, topical omilancor treatment decreased the metabolic upregulation in keratinocytes, keratinocyte hyperproliferation and expression of inflammatory markers. Omilancor is a promising topical, LANCL2-targeting therapeutic candidate for the treatment of PsO and other dermatology indications.
Dual-ligated metal organic framework as novel multifunctional nanovehicle for targeted drug delivery for hepatic cancer treatment
Authors: Fytory Mostafa , Arafa Kholoud K. , El Rouby Waleed M. A. , Farghali Ahmed A. , Abdel-Hafiez Mahmoud , El-Sherbiny Ibrahim M. 
Published: October 2021

AbstractIn the last decade, nanosized metal organic frameworks (NMOFs) have gained an increasing applicability as multifunctional nanocarriers for drug delivery in cancer therapy. However, only a limited number of platforms have been reported that can serve as an effective targeted drug delivery system (DDSs). Herein, we report rational design and construction of doxorubicin (DOX)-loaded nanoscale Zr (IV)-based NMOF (NH2-UiO-66) decorated with active tumor targeting moieties; folic acid (FA), lactobionic acid (LA), glycyrrhetinic acid (GA), and dual ligands of LA and GA, as efficient multifunctional DDSs for hepatocellular carcinoma (HCC) therapy. The success of modification was exhaustively validated by various structural, thermal and microscopic techniques. Biocompatibility studies indicated the safety of pristine NH2-UiO-66 against HSF cells whereas DOX-loaded dual-ligated NMOF was found to possess superior cytotoxicity against HepG2 cells which was further confirmed by flow cytometry. Moreover, fluorescence microscopy was used for monitoring cellular uptake in comparison to the non-ligated and mono-ligated NMOF. Additionally, the newly developed dual-ligated NMOF depicted a pH-responsiveness towards the DOX release. These findings open new avenues in designing various NMOF-based DDSs that actively target hepatic cancer to achieve precise therapy.
An observational study investigating the CRY1Δ11 variant associated with delayed sleep–wake patterns and circadian metabolic output
Authors: Smieszek Sandra P. , Brzezynski Jennifer L. , Kaden Alyssa R. , Shinn Jordan A. , Wang Jingyuan , Xiao Changfu , Polymeropoulos Christos , Özçelik Tayfun , Polymeropoulos Mihael H. 
Published: October 2021

AbstractWe conducted an observational research study to collect information on sleep–wake patterns from participants with a confirmed status of the cryptochrome circadian clock 1 (CRY1) splicing variant, CRY1Δ11 c.1657 + 3A > C, and their controls, defined as wild-type (WT) family members. Altogether, 67 participants were enrolled and completed this study in Turkey, recruited from a list of families with at least one CRY1-confirmed member. We measured sleep–wake patterns and metabolic output, specifically time and frequency of bowel movements, for all participants by daily post-sleep diaries over 28 days. The sleep diary measured self-reported bed time, wake time, midpoint of sleep, and latency to persistent sleep (LPS), and accounted for naps and awakenings for religious purposes. Wake time and midpoint of sleep were significantly later in the CRY1Δ11 variant group versus WT, and LPS was significantly greater in participants in the CRY1Δ11 variant group. The mean bed time on all nights of sleep was later in participants with a CRY1Δ11 variant versus WT. Additionally, participants with a CRY1Δ11 variant had significantly affected metabolic outputs, measured by later bowel movements than WT participants. These results demonstrate that, on average, individuals with the studied splicing variant experience pronounced delays in sleep period and circadian-related metabolic processes.
Effect of changes in Breslow thickness between the initial punch biopsy results and final pathology reports in acral lentiginous melanoma patients
Authors: Kim Tae Hyung , Kim Jin Cheol , Kwon Ji Eun , Kim You Chan , Choi Jee Woong 
Published: October 2021

AbstractAcral lentiginous melanoma (ALM) is the most common subtype of cutaneous melanoma among Asians; punch biopsy is widely performed for its diagnosis. However, the pathologic parameters evaluated via punch biopsy may not be sufficient for predicting disease prognosis compared to the parameters evaluated via excisional biopsy. We investigated whether changes in Breslow thickness (BT) between initial punch biopsy results and final pathology reports can affect the prognosis of ALM. Pathologic parameters were recorded from specimens acquired through the initial punch biopsy and wide excision. Patients were classified into two groups based on a change in Breslow depth: the BT increased or decreased on comparing the samples from the initial punch biopsy and final wide excision. We compared clinical characteristics, and a Cox regression model was used to identify independent prognostic factors influencing melanoma-specific death (MSD). Changes in BT did not affect MSD (hazard ratio [HR]: 0.55, P = 0.447). In multivariate analysis, a higher BT (> 2 mm) (HR: 9.93, P = 0.046) and nodal metastasis (HR: 5.66, P = 0.041) were significantly associated with an increased MSD risk. The use of punch biopsy did not affect MSD despite the inaccuracy of BT measurement as long as ALM was accurately diagnosed.
The effect of phacoemulsification performed with vitrectomy on choroidal vascularity index in eyes with vitreomacular diseases
Authors: Chun Heejeong , Kim Joo Young , Kwak Jae Hyuck , Kim Rae Young , Kim Mirinae , Park Young-Gun , Park Young-Hoon 
Published: October 2021

AbstractThis study evaluated the effects of cataract surgery combined with pars plana vitrectomy (ppV) on choroidal vascularity index (CVI) in eyes with epiretinal membrane (ERM) and full thickness macular hole (FTMH). Medical records of 132 eyes with ERM or FTMH were retrospectively reviewed and classified into a ppV group and a ppV combined with cataract surgery group (phaco + ppV group). The CVI were measured at baseline, 1, 3 and 6 months after the surgery, using the selected swept-source (SS) optical coherence tomography (OCT) scan passing through the central fovea, which was then segmented into luminal and stromal area by image binarization. The mean CVI of phaco + ppV group were 61.25 ± 1.97%, 61.66 ± 1.81%, and 62.30 ± 1.92% at baseline, 1 and 3 months, respectively (p < 0.001). The mean CVI of ppV group were 62.69 ± 1.92%, 62.03 ± 1.51%, and 61.45 ± 1.71% at baseline, 1 and 3 months, respectively (p < 0.001). The final CVI were measured at 6 months and compared with the baseline CVI. The mean CVI of phaco + ppV group were 61.21 ± 1.99% at baseline and 60.68 ± 2.02% at 6 months (p < 0.001). The mean CVI of ppV group were 62.93 ± 1.70% at baseline and 61.77 ± 1.74% at 6 months (p < 0.001). Vitrectomy significantly decreases CVI in vitreomacular diseases possibly due to the removal of vitreomacular traction or postoperative oxygenation change in the eye. On the contrary, combined surgery of vitrectomy and cataract surgery significantly increases CVI in the early stage of postoperative period, which suggests choroidal vascular dilatation or congestion due to postoperative inflammation. Although the CVI were measured lower than the baseline in the end, more thorough inflammation control may be essential after combined surgery.
Identification of loci associated with pathological outcomes in Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis using whole-genome sequence data
Authors: Canive Maria , Badia-Bringué Gerard , Vázquez Patricia , González-Recio Oscar , Fernández Almudena , Garrido Joseba M. , Juste Ramón A. , Alonso-Hearn Marta 
Published: October 2021

AbstractBovine paratuberculosis (PTB), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic granulomatous enteritis that affects cattle worldwide. According to their severity and extension, PTB-associated histological lesions have been classified into the following groups; focal, multifocal, and diffuse. It is unknown whether these lesions represent sequential stages or divergent outcomes. In the current study, the associations between host genetic and pathology were explored by genotyping 813 Spanish Holstein cows with no visible lesions (N = 373) and with focal (N = 371), multifocal (N = 33), and diffuse (N = 33) lesions in gut tissues and regional lymph nodes. DNA from peripheral blood samples of these animals was genotyped with the bovine EuroG MD Bead Chip, and the corresponding genotypes were imputed to whole-genome sequencing (WGS) data using the 1000 Bull genomes reference population. A genome-wide association study (GWAS) was performed using the WGS data and the presence or absence of each type of histological lesion in a case–control approach. A total of 192 and 92 single nucleotide polymorphisms (SNPs) defining 13 and 9 distinct quantitative trait loci (QTLs) were highly-associated (P ≤ 5 × 10−7) with the multifocal (heritability = 0.075) and the diffuse (heritability = 0.189) lesions, respectively. No overlap was seen in the SNPs controlling these distinct pathological outcomes. The identified QTLs overlapped with some QTLs previously associated with PTB susceptibility, bovine tuberculosis susceptibility, clinical mastitis, somatic cell score, bovine respiratory disease susceptibility, tick resistance, IgG level, and length of productive life. Pathway analysis with candidate genes overlapping the identified QTLs revealed a significant enrichment of the keratinization pathway and cholesterol metabolism in the animals with multifocal and diffuse lesions, respectively. To test whether the enrichment of SNP variants in candidate genes involved in the cholesterol metabolism was associated with the diffuse lesions; the levels of total cholesterol were measured in plasma samples of cattle with focal, multifocal, or diffuse lesions or with no visible lesions. Our results showed reduced levels of plasma cholesterol in cattle with diffuse lesions. Taken together, our findings suggested that the variation in MAP-associated pathological outcomes might be, in part, genetically determined and indicative of distinct host responses.
Determination of aortic pulse transit time based on waveform decomposition of radial pressure wave
Authors: Liu Wenyan , Song Daiyuan , Yao Yang , Qi Lin , Hao Liling , Yang Jun , Ning Hongxia , Xu Lisheng 
Published: October 2021

AbstractCarotid-femoral pulse transit time (cfPTT) is a widely accepted measure of central arterial stiffness. The cfPTT is commonly calculated from two synchronized pressure waves. However, measurement of synchronized pressure waves is technically challenging. In this paper, a method of decomposing the radial pressure wave is proposed for estimating cfPTT. From the radial pressure wave alone, the pressure wave can be decomposed into forward and backward waves by fitting a double triangular flow wave. The first zero point of the second derivative of the radial pressure wave and the peak of the dicrotic segment of radial pressure wave are used as the peaks of the fitted double triangular flow wave. The correlation coefficient between the measured wave and the estimated forward and backward waves based on the decomposition of the radial pressure wave was 0.98 and 0.75, respectively. Then from the backward wave, cfPTT can be estimated. Because it has been verified that the time lag estimation based on of backward wave has strong correlation with the measured cfPTT. The corresponding regression function between the time lag estimation of backward wave and measured cfPTT is y = 0.96x + 5.50 (r = 0.77; p < 0.001). The estimated cfPTT using radial pressure wave decomposition based on the proposed double triangular flow wave is more accurate and convenient than the decomposition of the aortic pressure wave based on the triangular flow wave. The significance of this study is that arterial stiffness can be directly estimated from a noninvasively measured radial pressure wave.
Capsule endoscopy findings reflect the gastrointestinal conditions of patients with systemic sclerosis
Authors: Iio Sumio , Oka Shiro , Tanaka Shinji , Sumioka Akihiko , Tsuboi Akiyoshi , Nojima Takaki , Hirata Shintaro , Matsuo Yoshimi , Sugiyama Eiji , Hide Michihiro , Arihiro Koji , Chayama Kazuaki 
Published: October 2021

AbstractSystemic sclerosis (SSc) is characterized by fibrosis of the skin and various internal organs. However, there is limited knowledge concerning small-bowel lesions. We evaluated the clinical state of patients with SSc according to the capsule endoscopy (CE) findings. Sixty-five consecutive patients with SSc (61 females; mean age, 64.3 years) underwent CE at Hiroshima University Hospital between April 2012 and December 2019. SSc was subclassified into diffuse and limited cutaneous SSc. Among the 65 patients, 55 (51 females; mean age, 64.5 years; diffuse cutaneous SSc, 27 patients) were evaluated for the presence of fibrosis in the gastrointestinal tract by biopsy. Small-bowel lesions were detected in 27 (42%) patients with SSc. Type 1b angioectasia (Yano-Yamamoto classification) was more frequent in limited cutaneous SSc patients (p = 0.0071). The average capsule transit time of the esophagus was significantly longer in diffuse cutaneous SSc patients (p = 0.0418). There were more cases of Type 1a angioectasia in SSc patients without fibrosis. The average capsule transit time of the esophagus was significantly longer in SSc patients with fibrosis. Thus, this study revealed that the frequency of small-bowel angioectasia and gastrointestinal motility in patients with SSc differed depending on SSc subclassification and the presence of fibrosis.
Association between functional genetic variants in retinoid X receptor-α/γand the risk of gestational diabetes mellitus in a southern Chinese population
Authors: Yu Xiang-yuan , Song Li-ping , Zheng Hui-ting , Wei Shu-dan , Wen Xiao-lan , Huang Bo , Liu Da-bin 
Published: October 2021

AbstractTo clarify the effect of retinoid X receptor-α/γ (RXR-α/γ) genes functional genetic variants (RXR-α rs4842194 G&gt;A, RXR-γ rs100537 A&gt;G and rs2134095 T&gt;C) on the risk of gestational diabetes mellitus (GDM), a case–control study with 573 GDM patients and 740 pregnant women with normal glucose tolerance was performed in Guangxi area of China. An odds ratio (OR) with its corresponding 95% confidence interval (CI) was used to assess the strengths of the association between genetic variation and GDM. After adjustment of age and pre-BMI, the logistic regression analysis showed that the rs2134095 was significantly associated with GDM risk (CC vs. TT/TC: adjusted OR = 0.71, 95% CI = 0.56–0.90) in all subjects, and this result remained highly significant after Bonferroni’s correction for multiple testing (P=0.004). The stratified analysis showed that rs2134095 was significantly associated with the risk of GDM among age &gt; 30 years (adjusted OR = 0.61, 95% CI = 0.39–0.97), BMI &gt; 22 kg/m2 (adjusted OR = 0.46, 95% CI = 0.30–0.70), systolic blood pressure (SBP) &gt; 120 mmHg (adjusted OR = 1.96, 95% CI = 1.14–3.36), glycosylated hemoglobin A1c (HbA1c) &lt; 6.5% (adjusted OR = 1.41, 95% CI = 1.11–1.78), TG ≤ 1.7 mmol/l (adjusted OR = 2.57, 95% CI = 1.45–4.53), TC ≤ 5.18 mmol/l (adjusted OR = 1.58, 95% CI = 1.13–2.22), high-density lipoprotein cholesterol (HDL-c) ≤ 1.5 mmol/l (adjusted OR = 1.70, 95% CI = 1.16–2.49) and low-density lipoprotein cholesterol (LDL-c) &gt; 3.12 mmol/l (adjusted OR = 1.47, 95% CI = 1.08–2.00) subjects, under the recessive genetic model. We also found that rs2134095 interacted with age (Pinteraction=0.039), pre-BMI (Pinteraction=0.040) and TG (Pinteraction=0.025) influencing individual’s genetic susceptibility to GDM. The rs2134095 T&gt;C is significantly associated with the risk of GDM by effect of a single locus and/or complex joint gene–gene and gene–environment interactions. Larger sample-size and different population studies are required to confirm the findings.
Sub‐bitstream packing based lightweight tiled streaming for 6 degree of freedom immersive video
Authors: Jeong Jong‐Beom , Lee Soonbin , Ryu Eun‐Seok 
Published: October 2021

AbstractThe MPEG immersive video (MIV) has been designed to remove the redundancies of multi‐view videos and merge the residuals into two textures and two geometry videos; therefore, four bitstreams and video decoders are required to provide six degrees of freedom (6DoF) in virtual reality (VR) in MIV. This letter proposes a sub‐bitstream packing method and realisation in tiled streaming for MIV to provide a single bitstream for legacy systems that have a single decoder without an increase in the server‐side storage. The proposed method divided the input videos into tiles in the compressed‐domain to accomplish two goals: (i) generate a merged bitstream using the existing four bitstreams for legacy systems and (ii) improve the quality of an immersive video by disabling the deblocking filter. For a player with one or few numbers of decoders, the extracted tile sub‐streams were merged by the proposed four specific mixed‐quality tiling and packing methods using a motion‐constrained tile set (MCTS), while complying with the high‐efficiency video coding (HEVC) standard. Experimental results demonstrated that the proposed sub‐stream packing method required a single decoder and exhibited 17.40% decoding time and 0.81% bjontegaard‐delta rate (BD‐rate) savings on Y‐PSNR saving compared to the non‐tiled streaming method.
Electromagnetic design and thermal analysis of module combined permanent magnet motor with wrapped type for mine ball mill
Authors: Xu Yingying , Zhang Bingyi , Feng Guihong 
Published: October 2021

AbstractWith the large‐scale development of the ball mill, the direct‐drive gearless ball mill has emerged, eliminating the limitation of the transmission torque of large gear. However, it is difficult to process, transport, install and maintain the gearless ball mill because of its vast volume, and the coils cannot be decoupled freely. Therefore, this work proposes a module combined permanent magnet motor with wrapped type for mine ball mill (MBMWT‐MCPMM). Based on the principle of removing coils, the stator is modularized. Each module can be regarded as a small rotary motor, which improves the reliability and fault tolerance of the motor. In this work, the structure and general design process of the MBMWT‐MCPMM are described, and the electromagnetic performance is simulated and analysed. The influence of different factors on the cooling effect of the motor and the temperature rise of different modules during fault‐tolerant operation are analysed. An 80 kW prototype is developed, and its experiments are carried out. The experimental results verify the validity of the related design and analysis.
Aufbauen statt aufputschen!
Authors: Vigl Sebastian 
Published: October 2021

SummaryDer Griff zu stimulierenden Substanzen wie Kaffee, Schwarztee & Co. bei Müdigkeit ist gängige Praxis, hilft nach Erfahrungen des Autors jedoch nur bei entsprechender energetischer Verfassung (siehe Tabelle 2). Die Nutzung von pflanzlichen Aufputschmitteln kann in einen schädlichen Gebrauch ausarten, wenn sie gewährleisten soll, dass Menschen über ihre gesunden Grenzen hinaus Leistung bringen. Aufgrund ihrer stimulierenden, stärkenden und entlastenden Wirkung sind Adaptogene Mittel der Wahl bei chronischer Erschöpfung und können je nach energetischer Verfassung mit anderen Pflanzen kombiniert werden.
Überraschende Echobefunde als Erstdiagnose eines angeborenen Herzfehlers im Erwachsenenalter
Authors: Huntgeburth Michael , Hohmann Christopher , von Scheidt Fabian , Meierhofer Christian 
Published: October 2021

ZusammenfassungAngeborene Herzfehler (AHF) werden teilweise erst im Erwachsenenalter diagnostiziert, entweder weil sie erst dann klinisch auffällig werden oder bei vorausgegangenen Untersuchungen übersehen wurden. Im Rahmen einer routinemäßigen transthorakalen Echokardiografie (TTE) können in der kardiologischen Praxis oder Klinik angeborene kardiale Anomalien leicht fehlgedeutet werden, da der Fokus in der Erwachsenenkardiologie überwiegend bei erworbenen Herzfehlern, einer Kardiomyopathie, Klappenerkrankungen oder einer systolischen oder diastolischen Dysfunktion liegt. Demgegenüber spielt eine thematische Auseinandersetzung mit AHF häufig eine nur untergeordnete Rolle, die Erwartungshaltung hinsichtlich angeborener kardialer Anomalien ist eher gering und die Sinne für mögliche AHF oft nicht geschärft. Dieser Artikel soll dazu beitragen, die Wahrscheinlichkeit des Erkennens eines AHF im Rahmen der TTE-Untersuchung zu erhöhen.