bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraoperative fluorescence imaging with aminolevulinic acid detects grossly occult breast cancer: a phase II randomized controlled trial
Authors: Ottolino-Perry Kathryn , Shahid Anam , DeLuca Stephanie , Son Viktor , Sukhram Mayleen , Meng Fannong , Liu Zhihui , Rapic Sara , Anantha Nayana Thalanki , Wang Shirley C. , Chamma Emilie , Gibson Christopher , Medeiros Philip J. , Majeed Safa , Chu Ashley , Wignall Olivia , Pizzolato Alessandra , Rosen Cheryl F. , Teene Liis Lindvere , Starr-Dunham Danielle , Kulbatski Iris , Panzarella Tony , Done Susan J. , Easson Alexandra M. , Leong Wey L. , DaCosta Ralph S. 
Published: July 2021

Abstract
Background
Re-excision due to positive margins following breast-conserving surgery (BCS) negatively affects patient outcomes and healthcare costs. The inability to visualize margin involvement is a significant challenge in BCS. 5-Aminolevulinic acid hydrochloride (5-ALA HCl), a non-fluorescent oral prodrug, causes intracellular accumulation of fluorescent porphyrins in cancer cells. This single-center Phase II randomized controlled trial evaluated the safety, feasibility, and diagnostic accuracy of a prototype handheld fluorescence imaging device plus 5-ALA for intraoperative visualization of invasive breast carcinomas during BCS.

Methods
Fifty-four patients were enrolled and randomized to receive no 5-ALA or oral 5-ALA HCl (15 or 30 mg/kg). Forty-five patients (n = 15/group) were included in the analysis. Fluorescence imaging of the excised surgical specimen was performed, and biopsies were collected from within and outside the clinically demarcated tumor border of the gross specimen for blinded histopathology.

Results
In the absence of 5-ALA, tissue autofluorescence imaging lacked tumor-specific fluorescent contrast. Both 5-ALA doses caused bright red tumor fluorescence, with improved visualization of tumor contrasted against normal tissue autofluorescence. In the 15 mg/kg 5-ALA group, the positive predictive value (PPV) for detecting breast cancer inside and outside the grossly demarcated tumor border was 100.0% and 55.6%, respectively. In the 30 mg/kg 5-ALA group, the PPV was 100.0% and 50.0% inside and outside the demarcated tumor border, respectively. No adverse events were observed, and clinical feasibility of this imaging device-5-ALA combination approach was confirmed.

Conclusions
This is the first known clinical report of visualization of 5-ALA-induced fluorescence in invasive breast carcinoma using a real-time handheld intraoperative fluorescence imaging device.

Trial registration
Clinicaltrials.gov identifier NCT01837225. Registered 23 April 2013.
DNA methylation under the major depression pathway predicts pediatric quality of life four-month post-pediatric mild traumatic brain injury
Authors: Duan Kuaikuai , Mayer Andrew R. , Shaff Nicholas A. , Chen Jiayu , Lin Dongdong , Calhoun Vince D. , Jensen Dawn M. , Liu Jingyu 
Published: July 2021

Abstract
Background
Major depression has been recognized as the most commonly diagnosed psychiatric complication of mild traumatic brain injury (mTBI). Moreover, major depression is associated with poor outcomes following mTBI; however, the underlying biological mechanisms of this are largely unknown. Recently, genomic and epigenetic factors have been increasingly implicated in the recovery following TBI.

Results
This study leveraged DNA methylation within the major depression pathway, along with demographic and behavior measures (features used in the clinical model) to predict post-concussive symptom burden and quality of life four-month post-injury in a cohort of 110 pediatric mTBI patients and 87 age-matched healthy controls. The results demonstrated that including DNA methylation markers in the major depression pathway improved the prediction accuracy for quality of life but not persistent post-concussive symptom burden. Specifically, the prediction accuracy (i.e., the correlation between the predicted value and observed value) of quality of life was improved from 0.59 (p = 1.20 × 10–3) (clinical model) to 0.71 (p = 3.89 × 10–5); the identified cytosine-phosphate-guanine sites were mainly in the open sea regions and the mapped genes were related to TBI in several molecular studies. Moreover, depression symptoms were a strong predictor (with large weights) for both post-concussive symptom burden and pediatric quality of life.

Conclusion
This study emphasized that both molecular and behavioral manifestations of depression symptoms played a prominent role in predicting the recovery process following pediatric mTBI, suggesting the urgent need to further study TBI-caused depression symptoms for better recovery outcome.
Prokaryotic diversity and potentially pathogenic bacteria in vended foods and environmental samples
Authors: Muriuki Susan W. , Rengan Michael S. , Budambula Nancy L. M. 
Published: July 2021

Abstract
Purpose
Ready-to-eat fast food vending outlets provide a cheap and readily available food. Foodborne diseases have been previously reported in Embu, Kenya, but data on the prokaryotic metagenome in vended foods is scanty. This study aimed to determine the prokaryotic diversity in fruits, vegetable salad, African sausage, chips (potato fries), fried fish, roasted beef (meat), smokies, samosa, soil, and water collected from food vendors and the surrounding environment in Embu Town and Kangaru Market.

Methods
The study used 454 pyrosequencing, Illumina high-throughput sequencing of 16S rRNA gene in the analysis of total community DNA extracted from samples using the phenol-chloroform method. The 16S rRNA gene variable region (V4-V7) of the extracted DNA was amplified and library construction performed. Sequence analysis was done using QIIME2. Hierarchical clustering of samples, diversity indices, rarefaction curves, and Venn diagrams were generated using the R programming language in R software version 3.6.3.

Results
Bacterial operational taxonomic units (OUTs) were distributed in Proteobacteria (52.81%), Firmicutes (31.16%), and Lentisphaerae (0.001%). The OTUs among archaea were Candidatus Nitrososphaera (63.56%) and Nitrososphaera spp. (8.77%). Brucella spp. and Bacillus cereus associated with foodborne diseases were detected. Potential pathogens, Rickettsia spp. in risk group 2 and Brucella spp. in risk group 3, were detected. Uncultured Candidatus Koribacter and Candidatus Solibacter were also detected in the food samples. There was a significant difference in the microbial community structure among the sample types (P<0.1).

Conclusion
The results demonstrated the presence of some prokaryotes that are associated with food spoilage or foodborne diseases in vended foods and environmental samples. This study also detected uncultured prokaryotes. The presence of potential pathogens calls for stringent hygiene measures in food vending operations.
Clinical improvement of encapsulating peritoneal sclerosis after challenging course and 6 months of total parenteral nutrition in child with nephronophthisis: a case report
Authors: AlZabali Saeed Mohammed , AlAnazi Abdulkarim , Rahim Khawla A. , Faqeehi Hassan Y. 
Published: July 2021

Abstract
Background
Encapsulating peritoneal sclerosis is a rare but potentially lethal complication of long-term peritoneal dialysis that is associated with significant morbidity and mortality. The occurrence of encapsulating peritoneal sclerosis varies worldwide, but is increased in patients maintained on peritoneal dialysis for 5–8 years. The etiology of encapsulating peritoneal sclerosis remains unidentified, and a high index of clinical suspicion is required for diagnosis.

Case presentation
We report a 5-year-old Saudi female with end-stage renal disease secondary to nephronophthisis type 2. She underwent peritoneal dialysis for 30 months, with four episodes of peritonitis. She presented with clinical signs of peritonitis. Three days later, she developed septic shock, which required pediatric intensive care unit admission. The peritoneal dialysis catheter was removed because of refractory peritonitis. Her course was complicated by small bowel perforation, and severe adhesions were revealed on abdominal ultrasound and computed tomography, consistent with a diagnosis of EPS. This finding was later confirmed by diagnostic laparotomy performed twice and complicated by recurrent abdominal wall fistula. She received total parenteral nutrition for 6 months and several courses of antibiotics. The patient received supportive treatment including nutritional optimization and treatment for infection. No other treatments, such as immunosuppression, were administered to avoid risk of infection. Following a complicated hospital course, the patient restarted oral intake after 6 months of total parenteral nutrition dependency. Her abdominal fistula resolved completely, and she was maintained on hemodialysis for few years before she received a kidney transplant.

Conclusion
When treating patients using peritoneal dialysis, it is important to consider encapsulating peritoneal sclerosis with refractory peritonitis, which is not always easy to identify, particularly if the patient has been maintained on peritoneal dialysis for less than 3 years. Early identification of encapsulating peritoneal sclerosis and appropriate conservative treatment, including nutritional optimization and treatment of infections, are essential to achieve a better prognosis.
Medication safety knowledge, attitude, and practice among hospital pharmacists in tertiary care hospitals in Saudi Arabia: a multi-center study
Authors: AL-Mutairi Azizah , AlFayyad Isamme , Altannir Youssef , Al-Tannir Mohamad 
Published: July 2021

Abstract
Background
Pharmacovigilance (PV) demarcates all actions involving the detection and prevention of adverse drug reactions (ADR) for marketed drugs. However, ADRs are considerably underreported worldwide and continue to be a major concern to health care systems. This study aims to assess the knowledge, attitude, and perception of hospital pharmacists regarding medication safety concerning PV and ADRs across multiple tertiary care centers around Saudi Arabia.

Methods
This cross-sectional study was conducted between July 2019 and January 2020. Pharmacists working in the tertiary care centers of Riyadh City, Saudi Arabia were asked to participate in the study. A self-administered questionnaire was used to conduct this study, it consisted of: 63 questions out of which 19 questions were knowledge-based, 15 were attitude-based, and 29 were practice-based questions.

Results
A total of 350 pharmacists were distributed and 289 agreed to participate, giving a response rate of 82.6%. Most pharmacists were aware of the concept of VP and its functions (96.5%) and (87.2%), respectively. Moreover, 90% said that ADR can be preventable and non-preventable. However, the findings revealed inadequate knowledge about the overall PV field, where the majority of the pharmacists failed to correctly answer questions related to independent ADRs treatment, Augmented drug reaction, the international location of ADR, and the World Health Organization “online database” for reporting ADRs. Moreover, incomplete and/or wrong answers were recorded for questions that included single or multiple correct answers. Regarding the participants” attitude, 96.9% were interested in ADR reporting, agreeing that ADR is important to enable safe drug usage. Although a general positive attitude was recorded, pharmacists have stated that the three main barriers that hinder reporting ADRs are: unavailability of information about ADRs, lack of awareness about the need to report ADRs, and lack of time. Concerning practice, 69.2% said they received training in ADRs reporting, and 70% have reported ADRs more than once a week.

Conclusion
Surveyed pharmacists from Riyadh hospitals showed narrow knowledge of the PV field. However, a positive attitude and satisfactory practice was observed among pharmacists. These findings warrant the need for educational programs and an encouraging environment for ADR reporting to increase ADR reporting rates and support PV activities in Saudi Arabia.
Efficiency evaluation of BRICS’s national innovation systems based on bias-corrected network data envelopment analysis
Authors: Alnafrah Ibrahim 
Published: July 2021

AbstractThe very limited studies that tried to measure the efficiency of national innovation systems (NISs) in BRICS economies were limited to the assumption that the innovation process at national level consists of one stage only and got different and conflicting results. Therefore, this study endeavours to measure the efficiency of sub-processes within the BRICS’s NISs and identify where the system failure lies in each NIS. Bias-corrected network data envelopment analysis (DEA) is used to measure the efficiency of total NIS and the efficiency of the other sub-processes within the system: (1) knowledge production process (KPP), and (2) knowledge commercialization process (KCP). The results showed that NISs in BRICS economies suffer from low performance in commercializing their outputs of universities and research organizations. While, on the other hand, their performance in creating scientific and technical knowledge is good in comparison to other studied countries. We suggest that the reason behind this imbalance is the network system failure associated with weak institutions and high uncertainty in the economy. In this study, we argue that the problem in BRICS NISs is not a problem of resources, but it is a problem of system management and institutions. Some bridging policies are suggested to be adopted by BRICS economies to improve their innovation performance and overcome the system failure of weak links between universities and industry.
Household food security among Hadza hunter-gatherers in Mkalama district, Tanzania
Authors: Safari John Godfrey , Nkua Aron Joseph , Masanyiwa Zacharia Samwel 
Published: July 2021

Abstract
Background
Food insecurity is a widespread public health concern in many communities of sub-Saharan Africa. This study involved the Hadza hunter-gatherers of Tanzania, the only ethnic group in the country that has traditionally subsisted on hunting and gathering. In recent years, however, these communities have adopted mixed foraging economies. Information on how this change affects household food security is rather limited. The aim of this study was to assess the status of food security and the factors influencing household food security in the Hadza hunter-gatherer communities.

Methods
A cross-sectional study of 200 households was conducted in Mkalama district, Tanzania. Sampled householders represented individuals whose livelihood is mainly dependent on foraging (n = 129), beekeeping (n = 30) and farming (n = 41). Food security was measured by assessing food availability (Months of Adequate Food Provisioning (MAHFP)), food access (Household Food Insecurity Access Scale (HFIAS)) and food utilization (Dietary Diversity Scores (DDS)).

Results
Mean MAHFP was lower (p = 0.000) in predominantly foraging households (8.4 ± 1.1) compared with those involved in beekeeping (8.7 ± 1.6) or farming (9.6 ± 1.9). Based on HFIAS indicator, the prevalence of food insecurity varied with the household’s main activity (83.0% foraging, 46.7% beekeeping and 26.8% farming). Further, regression analyses show that the farming households were more likely to be food secure than the foraging households (OR = 10.7, p = 0.01). Dietary diversity scores also varied significantly with household’s main activity. About 65% of households (86% foraging, 63.3% beekeeping and 2.4% farming) consumed diets below the critical value of ≤ 4 food groups 24 h prior to survey. Social demographic characteristics and livelihood options are strong predictors of household food security.

Conclusion
All indicators used to assess food security point to high level of food insecurity in households mainly subsisting on foraging compared with beekeeping and farming. The primary dependence on foraging is associated with a longer period of food shortage, high prevalence of food insecurity conditions and low consumption of food varieties. Livelihood diversification coupled with provision of agricultural support services is necessary for the development of a secure future of the Hadza communities.
COVID-19-related violence trend data challenges & a resource for injury researchers
Authors: Rochford Hannah I. , Brooks Kaleb , Berg Mark , Peek-Asa Cori 
Published: July 2021

AbstractPublished works have raised concerns that certain violent behaviors and firearm acquisition have encountered dramatic increases since the onset of COVID-19. While these works provide important preliminary insights, they lack the empirical robustness necessary to inform a targeted societal response. Having the ability to perform the research needed to support evidence-based policy requires that data at national, state and local-levels be accessible and of sufficient quality. While related, robust data sources do arguably exist, their availability may come long after the window for effective prevention and intervention efforts has closed or may otherwise present with quality limitations, leaving populations at risk for various forms of violence without the support of protective policies.The University of Iowa Injury Prevention Research Center and the Public Policy Center has compiled a compendium of secondary data sources in an effort to promote exploration of relationships between the COVID-19 pandemic and rates of injury and violence. The forms of violence and firearm-related behavior that were identified as being at risk for amplification given the social stress, economic stress and isolation associated with the public health emergency period included: firearm acquisition, firearm violence, intimate partner violence and family violence.
Response of a hydrothermal system to escalating phreatic unrest: the case of Turrialba and Irazú in Costa Rica (2007–2012)
Authors: Rouwet D. , Mora-Amador R. , Ramírez C. , González G. , Baldoni E. , Pecoraino G. , Inguaggiato S. , Capaccioni B. , Lucchi F. , Tranne C. A. 
Published: July 2021

AbstractThis study presents the first hydrogeochemical model of the hydrothermal systems of Turrialba and Irazú volcanoes in central Costa Rica, manifested as thermal springs, summit crater lakes, and fumarolic degassing at both volcanoes. Our period of observations (2007–2012) coincides with the pre- and early syn-phreatic eruption stages of Turrialba volcano that resumed volcanic unrest since 2004, after almost 140 years of quiescence. Peculiarly, the generally stable Irazú crater lake dropped its level during this reawakening of Turrialba. The isotopic composition of all the discharged fluids reveals their Caribbean meteoric origin. Four groups of thermal springs drain the northern flanks of Turrialba and Irazú volcanoes into two main rivers. Río Sucio (i.e. “dirty river”) is a major rock remover on the North flank of Irazú, mainly fed by the San Cayetano spring group. Instead, one group of thermal springs discharges towards the south of Irazú. All thermal spring waters are of SO4-type (i.e. steam-heated waters), none of the springs has, however, a common hydrothermal end-member. A water mass budget for thermal springs results in an estimated total output flux of 187 ± 37 L/s, with 100 ± 20 L/s accounted for by the San Cayetano springs. Thermal energy release is estimated at 110 ± 22 MW (83.9 ± 16.8 MW by San Cayetano), whereas the total rock mass removal rate by chemical leaching is ~ 3000 m3/year (~ 2400 m3/year by San Cayetano-Río Sucio). Despite Irazú being the currently less active volcano, it is a highly efficient rock remover, which, on the long term can have effects on the stability of the volcanic edifice with potentially hazardous consequences (e.g. flank collapse, landslides, phreatic eruptions). Moreover, the vapor output flux from the Turrialba fumaroles after the onset of phreatic eruptions on 5 January 2010 showed an increase of at least ~ 260 L/s above pre-eruptive background fumarolic vapor fluxes. This extra vapor loss implies that the drying of the summit hydrothermal system of Turrialba could tap deeper than previously thought, and could explain the coincidental disappearance of Irazú’s crater lake in April 2010.
Chemical-free recovery of crude protein from livestock manure digestate solid by thermal hydrolysis
Authors: Tasaki Ken 
Published: July 2021

AbstractProtein is becoming an increasingly important resource for a variety of commercial applications. Yet, a large volume of protein is being wasted. Notably, livestock manure solids have a significant content of protein which is not only underutilized, but prone to runoff and eventual breakdown to reactive nitrogen compounds, contributing to eutrophication. It would be desirable to remove protein before it causes environmental hazards and then convert it to value-added commercial applications. We have developed a novel thermal hydrolysis process (THP) to extract crude protein from livestock manure solid, or manure digestate solid (MDS) in particular, without the use of any chemical. We demonstrate the versatility of our new process to control the molecular weight (MW) distribution of the extracted protein hydrolysate (PH). The antioxidant activity of the crude protein hydrolysate (CPH) has been examined through Oxygen Radical Absorbance Capacity Assay. The results have shown that our CPH had its antioxidant capacity against the peroxyl radical similar to that of vitamin E and exhibited almost 7 times as strong inhibition against the hydroxyl radical as vitamin E. We also evaluated the nutritional value of our PH by analyzing its amino acid composition and the MW distribution through amino acid analysis, SDS-PAGE, and MALDI-TOF mass spectroscopy. The characterizations have revealed that the PH recovered from MDS had 2.5 times as much essential amino acids as soybean meal on dry matter basis, with the MW distribution ranging from over a 100 Da to 100 KDa. Finally, the protein powder was prepared from the extracted CPH solution and its composition was analyzed.
Optimizing reconstruction parameters for quantitative 124I-PET in the presence of therapeutic doses of 131I
Authors: Fanchon Louise M. , Beattie Bradley J. , Pentlow Keith , Larson Steven M. , Humm John L. 
Published: July 2021

Abstract
Background
The goal of this work was to determine the quantitative accuracy and optimal reconstruction parameters for 124I-PET imaging in the presence of therapeutic levels of 131I. In this effort, images were acquired on a GE D710 PET/CT scanner using a NEMA IEC phantom with spheres containing 124I and increasing amounts of 131I activity in the background. At each activity level, two scans were acquired, one with the phantom centered in the field of view (FOV) and one 11.2 cm off-center. Reconstructions used an ordered subset expectation maximization algorithm with up to 100 iterations of 16 subsets, with and without time-of-flight (TOF) information. Results were evaluated visually and by comparing the 124I activity relative to the scan performed in the absence of 131I.

Results
131I within the FOV added to the randoms rate, to dead time, and to pile-up within the detectors. Using our standard clinical reconstruction parameters, the image quality and quantitative accuracy suffered at 131I activities above 1.4 GBq. Convergence rates slowed progressively in the presence of increasing amounts of 131I for both TOF and nonTOF reconstructions. TOF reconstructions converged more quickly than nonTOF but often towards erroneous concentrations. Iterating nonTOF reconstructions to convergence produced quantitatively accurate images except for the off-center phantom at the very highest level of background 131I tested.

Conclusions
This study shows that quantitative PET is feasible in the presence of large amounts of 131I. The high randoms fractions resulted in slow reconstruction convergence and negatively impacted TOF corrections and/or the accuracy of TOF information. Therefore, increased iterations and nonTOF reconstructions are recommended.