bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rs1h−/y exon 3-del rat model of X-linked retinoschisis with early onset and rapid phenotype is rescued by RS1 supplementation
Authors: Zeng Yong , Qian Haohua , Campos Maria Mercedes , Li Yichao , Vijayasarathy Camasamudram , Sieving Paul A. 
Published: September 2021

AbstractAnimal models of X-linked juvenile retinoschisis (XLRS) are valuable tools for understanding basic biochemical function of retinoschisin (RS1) protein and to investigate outcomes of preclinical efficacy and toxicity studies. In order to work with an eye larger than mouse, we generated and characterized an Rs1h−/y knockout rat model created by removing exon 3. This rat model expresses no normal RS1 protein. The model shares features of an early onset and more severe phenotype of human XLRS. The morphologic pathology includes schisis cavities at postnatal day 15 (p15), photoreceptors that are misplaced into the subretinal space and OPL, and a reduction of photoreceptor cell numbers by p21. By 6 mo age only 1–3 rows of photoreceptors nuclei remain, and the inner/outer segment layers and the OPL shows major changes. Electroretinogram recordings show functional loss with considerable reduction of both the a-wave and b-wave by p28, indicating early age loss and dysfunction of photoreceptors. The ratio of b-/a-wave amplitudes indicates impaired synaptic transmission to bipolar cells in addition. Supplementing the Rs1h−/y exon3-del retina with normal human RS1 protein using AAV8-RS1 delivery improved the retinal structure. This Rs1h−/y rat model provides a further tool to explore underlying mechanisms of XLRS pathology and to evaluate therapeutic intervention for the XLRS condition.
Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-κB signaling pathway
Authors: Hu Su-Qin , Liu Dian-Long , Li Chun-Rui , Xu Ya-Hui , Hu Ke , Cui Li-Dan , Guo Jian 
Published: September 2021

AbstractAkt and nuclear factor kappa B (NF-κB) signaling pathways are involved in germ cell apoptosis and inflammation after testicular heat stress (THS). We observed that after THS induced by the exposure of rat testes to 43 °C for 20 min, their weight decreased, the fraction of apoptotic testicular germ cells significantly increased, and the proliferation of germ cells was inhibited. In addition, THS lowered serum testosterone (T) level, whereas the levels of follicle stimulating hormone and luteinizing hormone were not significantly changed. The ultrastructure of the seminiferous tubules became abnormal after THS, the structure of the blood-testis barrier (BTB) became loose, and the Sertoli cells showed a trend of differentiation. The level of phosphorylated Akt was reduced, whereas the amount of phosphorylated NF-κB p65 was augmented by THS. Wuzi-Yanzong (WZYZ), a classic Chinese medicine prescription for the treatment of male reproductive dysfunctions, alleviated the changes induced by THS. In order to determine the mechanism of action of WZYZ, we investigated how this preparation modulated the levels of T, androgen receptor (AR), erythropoietin (EPO), EPO receptor, and Tyro-3, Axl, and Mer (TAM) family of tyrosine kinase receptors. We found that WZYZ activated the Akt pathway, inhibited the Toll-like receptor/MyD88/NF-κB pathway, and repaired the structure of BTB by regulating the levels of T, AR, TAM receptors, and EPO. In conclusion, these results suggest that WZYZ activates the Akt pathway and inhibits the NF-κB pathway by acting on the upstream regulators, thereby improving spermatogenesis deficit induced by THS.
Mental health of new undergraduate students before and after COVID-19 in China
Authors: Lu Peng , Yang Lei , Wang Chongjian , Xia Guoxin , Xiang Hao , Chen Gongbo , Jiang Ning , Ye Tingting , Pang Yucheng , Sun Hongwei , Yan Lailai , Su Zhenguo , Heyworth Jane , Huxley Rachel , Fisher Jane , Li Shanshan , Guo Yuming 
Published: September 2021

AbstractThe purpose of this study was to examine the changes in severity of anxiety and depression symptoms, stress and sleeping quality after three months of mass quarantine for COVID-19 among undergraduate fresh students compared to their pre-COVID-19 measures. We used participants from the Chinese Undergraduate Cohort (CUC), a national prospective longitudinal study to examine the changes in anxiety and depression symptoms severity, stress and sleep quality after being under mass quarantine for three months. Wilcoxon matched pair signed-rank test was used to compare the lifestyle indicators. Severity of anxiety, depression symptoms, stress and sleep quality were compared with Wilcoxon signed-rank test. We used generalized estimating equation (GEE) to further quantify the change in mental health indicators and sleep quality after the COVID-19 mass quarantine compared to baseline. This study found that there was no deterioration in mental health status among Chinese new undergraduate students in 2020 after COVID-19 mass quarantine compared with the baseline measures in 2019. There was an improvement in sleep quality and anxiety symptoms. After adjusting for age, sex, exercise habit, time spent on mobile gadgets, and time spent outdoors, year 2020 was significantly associated with severity of depression symptoms in males (OR:1.52. 95%CI:1.05–2.20, p-value = 0.027). Year 2020 was significantly associated with the improvement of sleeping quality in total (OR:0.45, 95%CI:0.38–0.52, p < 0.001) and in all the subgroups. This longitudinal study found no deterioration in mental health status among Chinese new undergraduate students after three months of mass quarantine for COVID-19.
Direct evidence of sex and a hypothesis about meiosis in Symbiodiniaceae
Authors: Figueroa R. I. , Howe-Kerr L. I. , Correa A. M. S. 
Published: September 2021

AbstractDinoflagellates in the family Symbiodiniaceae are obligate endosymbionts of diverse marine invertebrates, including corals, and impact the capacity of their hosts to respond to climate change-driven ocean warming. Understanding the conditions under which increased genetic variation in Symbiodiniaceae arises via sexual recombination can support efforts to evolve thermal tolerance in these symbionts and ultimately mitigate coral bleaching, the breakdown of the coral-Symbiodiniaceae partnership under stress. However, direct observations of meiosis in Symbiodiniaceae have not been reported, despite various lines of indirect evidence that it occurs. We present the first cytological evidence of sex in Symbiodiniaceae based on nuclear DNA content and morphology using Image Flow Cytometry, Cell Sorting and Confocal Microscopy. We show the Symbiodiniaceae species, Cladocopium latusorum, undergoes gamete conjugation, zygote formation, and meiosis within a dominant reef-building coral in situ. On average, sex was detected in 1.5% of the cells analyzed (N = 10,000–40,000 cells observed per sample in a total of 20 samples obtained from 3 Pocillopora colonies). We hypothesize that meiosis follows a two-step process described in other dinoflagellates, in which diploid zygotes form dyads during meiosis I, and triads and tetrads as final products of meiosis II. This study sets the stage for investigating environmental triggers of Symbiodiniaceae sexuality and can accelerate the assisted evolution of a key coral symbiont in order to combat reef degradation.
Macrophage autophagy protects against hepatocellular carcinogenesis in mice
Authors: Deust Anthony , Chobert Marie-Noële , Demontant Vanessa , Gricourt Guillaume , Denaës Timothé , Thiolat Allan , Ruiz Isaac , Rodriguez Christophe , Pawlotsky Jean-Michel , Teixeira-Clerc Fatima 
Published: September 2021

AbstractAutophagy is a lysosomal degradation pathway of cellular components that regulates macrophage properties. Macrophages are critically involved in tumor growth, metastasis, angiogenesis and immune suppression. Here, we investigated whether macrophage autophagy may protect against hepatocellular carcinoma (HCC). Experiments were performed in mice with deletion of the autophagy gene Atg5 in the myeloid lineage (ATG5Mye−/− mice) and their wild-type (WT) littermates. As compared to WT, ATG5Mye−/− mice were more susceptible to diethylnitrosamine (DEN)-induced hepatocarcinogenesis, as shown by enhanced tumor number and volume. Moreover, DEN-treated ATG5Mye−/− mice exhibited compromised immune cell recruitment and activation in the liver, suggesting that macrophage autophagy invalidation altered the antitumoral immune response. RNA sequencing showed that autophagy-deficient macrophages sorted from DEN mice are characterized by an enhanced expression of immunosuppressive markers. In vitro studies demonstrated that hepatoma cells impair the autophagy flux of macrophages and stimulate their expression of programmed cell death-ligand 1 (PD-L1), a major regulator of the immune checkpoint. Moreover, pharmacological activation of autophagy reduces hepatoma cell-induced PD-L1 expression in cultured macrophages while inhibition of autophagy further increases PD-L1 expression suggesting that autophagy invalidation in macrophages induces an immunosuppressive phenotype. These results uncover macrophage autophagy as a novel protective pathway regulating liver carcinogenesis.
Reversible insulin resistance helps Bactrian camels survive fasting
Authors: Guo Fucheng , Si Rendalai , Li Quanyun , Hai Le , Yi Li , He Jing , Ming Liang , Ji Rimutu 
Published: September 2021

AbstractCamels have hunger tolerance and can adapt to the severe environment of the desert. Through the comparison of insulin signalling pathway genes in different tissues in different eating periods (feeding, fasting and recovery feeding), it was found that IRS1, PIK3CB, PIK3R1 and SLC2A4 expression was significantly downregulated in the fore hump and hind hump during the fasting period. In addition, there was no difference in serum insulin levels among the three stages. However, the serum leptin and adiponectin levels decreased significantly during fasting. Additionally, insulin tolerance tests during the three stages showed that camels were insensitive to insulin during fasting. Further study of the serum metabolites showed that serum branched-chain and aromatic amino acid levels increased during the fasting period. Finally, analysis of microbial diversity in camel faeces at different stages showed that during the fasting period, the proportion of Firmicutes and Actinobacteria increased, while that of Bacteroides and the butyrate-producing bacterium Roseburia decreased. The results of this study show that fasting is accompanied by changes in the activation of insulin pathways in various camel tissues, normal insulin levels, and increased lipolysis and insulin resistance, which return to normal after eating.
FDG PET biomarkers for prediction of survival in metastatic melanoma prior to anti-PD1 immunotherapy
Authors: Flaus A. , Habouzit V. , De Leiris N. , Vuillez J. P. , Leccia M. T. , Perrot J. L. , Prevot N. , Cachin F. 
Published: September 2021

AbstractOur aim was to analyse whether biomarkers extracted from baseline 18F-FDG PET before anti-PD1 treatment contribute to prognostic survival information for early risk stratification in metastatic melanoma. Fifty-six patients, without prior systemic treatment, BRAF wild type, explored using 18F-FDG PET were included retrospectively. Our primary endpoint was overall survival (OS). Total metabolic tumoral volume (MTV) and forty-one IBSI compliant parameters were extracted from PET. Parameters associated with outcome were evaluated by a cox regression model and when significant helped build a prognostic score. Median follow-up was 22.1 months and 21 patients died. Total MTV and long zone emphasis (LZE) correlated with shorter OS and served to define three risk categories for the prognostic score. For low, intermediate and high risk groups, survival rates were respectively 91.1% (IC 95 80–1), 56.1% (IC 95 37.1–85) and 19% (IC 95 0.06–60.2) and hazard ratios were respectively 0.11 (IC 95 0.025–0.46), P = 0.0028, 1.2 (IC 95 0.48–2.8), P = 0.74 and 5.9 (IC 95 2.5–14), P < 0.0001. To conclude, a prognostic score based on total MTV and LZE separated metastatic melanoma patients in 3 categories with dramatically different outcomes. Innovative therapies should be tested in the group with the lowest prognosis score for future clinical trials.
Reduced B7-H3 expression by PAX3-FOXO1 knockdown inhibits cellular motility and promotes myogenic differentiation in alveolar rhabdomyosarcoma
Authors: Kanayama Takuyo , Miyachi Mitsuru , Sugimoto Yohei , Yagyu Shigeki , Kikuchi Ken , Tsuchiya Kunihiko , Iehara Tomoko , Hosoi Hajime 
Published: September 2021

AbstractB7-H3 (also known as CD276) is associated with aggressive characteristics in various cancers. Meanwhile, in alveolar rhabdomyosarcoma (ARMS), PAX3-FOXO1 fusion protein is associated with increased aggressiveness and poor prognosis. In the present study, we explored the relationship between PAX3-FOXO1 and B7-H3 and the biological roles of B7-H3 in ARMS. Quantitative real time PCR and flow cytometry revealed that PAX3-FOXO1 knockdown downregulated B7-H3 expression in all the selected cell lines (Rh-30, Rh-41, and Rh-28), suggesting that PAX3-FOXO1 positively regulates B7-H3 expression. Gene expression analysis revealed that various genes and pathways involved in chemotaxis, INF-γ production, and myogenic differentiation were commonly affected by the knockdown of PAX3-FOXO1 and B7-H3. Wound healing and transwell migration assays revealed that both PAX3-FOXO1 and B7-H3 were associated with cell migration. Furthermore, knockdown of PAX3-FOXO1 or B7-H3 induced myogenin expression in all cell lines, although myosin heavy chain induction varied depending on the cellular context. Our results indicate that PAX3-FOXO1 regulates B7-H3 expression and that PAX3-FOXO1 and B7-H3 are commonly associated with multiple pathways related to an aggressive phenotype in ARMS, such as cell migration and myogenic differentiation block.
2-step reaction kinetics for hydrogen absorption into bulk material via dissociative adsorption on the surface
Authors: Yakabe Taro , Imamura Gaku , Yoshikawa Genki , Miyauchi Naoya , Kitajima Masahiro , Itakura Akiko N. 
Published: September 2021

AbstractWe have demonstrated that the process of hydrogen absorption into a solid experimentally follows a Langmuir-type (hyperbolic) function instead of Sieverts law. This can be explained by independent two theories. One is the well-known solubility theory which is the basis of Sieverts law. It explains that the amount of hydrogen absorption can be expressed as a Langmuir-type (hyperbolic) function of the square root of the hydrogen pressure. We have succeeded in drawing the same conclusion from the other theory. It is a 2-step reaction kinetics (2sRK) model that expresses absorption into the bulk via adsorption on the surface. The 2sRK model has an advantage to the solubility theory: Since it can describe the dynamic process, it can be used to discuss both the amount of hydrogen absorption and the absorption rate. Some phenomena with absorption via adsorption can be understood in a unified manner by the 2sRK model.
Clinical impact of visually assessed right ventricular dysfunction in patients with septic shock
Authors: Hiraiwa Hiroaki , Kasugai Daisuke , Ozaki Masayuki , Goto Yukari , Jingushi Naruhiro , Higashi Michiko , Nishida Kazuki , Kondo Toru , Furusawa Kenji , Morimoto Ryota , Okumura Takahiro , Matsuda Naoyuki , Matsui Shigeyuki , Murohara Toyoaki 
Published: September 2021

AbstractWe retrospectively analyzed data from the Medical Information Mart for Intensive Care-III critical care database to determine whether visually-assessed right ventricular (RV) dysfunction was associated with clinical outcomes in septic shock patients. Associations between visually-assessed RV dysfunction by echocardiography and in-hospital mortality, lethal arrhythmia, and hemodynamic indicators to determine the prognostic value of RV dysfunction in patients with septic shock were analyzed. Propensity score analysis showed RV dysfunction was associated with increased risk of in-hospital death in patients with septic shock (adjusted odds ratio [OR] 2.15; 95% confidence interval [CI] 1.99–2.32;P < 0.001). In multivariate logistic regression analysis, RV dysfunction was associated with in-hospital death (OR 2.19; 95% CI 1.91–2.53;P < 0.001), lethal arrhythmia (OR 2.19; 95% CI 1.34–3.57;P < 0.001), and tendency for increased blood lactate levels (OR 1.31; 95% CI 1.14–1.50;P < 0.001) independent of left ventricular (LV) dysfunction. RV dysfunction was associated with lower cardiac output, pulmonary artery pressure index, and RV stroke work index. In patients with septic shock, visually-assessed RV dysfunction was associated with in-hospital mortality, lethal arrhythmia, and circulatory insufficiency independent of LV dysfunction. Visual assessment of RV dysfunction using echocardiography might help to identify the short-term prognosis of patients with septic shock by reflecting hemodynamic status.
Structure of junctional epithelium is maintained by cell populations supplied from multiple stem cells
Authors: Tanaka Keisuke , Tanaka Junichi , Aizawa Ryo , Kato-Tanaka Mayu , Ueno Hiroo , Mishima Kenji , Yamamoto Matsuo 
Published: September 2021

AbstractThe junctional epithelium (JE) is an epithelial component that attaches directly to the tooth surface and performs the unique function of protecting against bacterial infections; its destruction causes inflammation of the periodontal tissue and loss of alveolar bone. A recent study that used the single-color lineage tracing method reported that JE is maintained by its stem cells. However, the process by which individual stem cells form the entire JE around a whole tooth remains unclear. Using a 4-color lineage tracing method, we performed a detailed examination of the dynamics of individual stem cells that constitute the entire JE. The multicolor lineage tracing method showed that single-color areas, which were derived from each cell color, replaced all the constituent JE cells 168 d after the administration of tamoxifen. The horizontal section of the first molar showed that the single-color areas in the JE expanded widely. We detected putative stem cells at the external basal layer farthest from the enamel. In this study, JE cells that were supplied from different stem cells were visualized as individual monochromatic regions, and the JE around the first molar was maintained by several JE-specific stem cells. These findings indicated that the JE consisted of several cell populations that were supplied from their multiple stem cells and could help to explore the mechanisms involved in periodontal tissue homeostasis.
The 3D organisation of mitochondria in primate photoreceptors
Authors: Hayes Matthew J. , Tracey-White Dhani , Kam Jaimie Hoh , Powner Michael B. , Jeffery Glen 
Published: September 2021

AbstractVertebrate photoreceptors contain large numbers of closely-packed mitochondria which sustain the high metabolic demands of these cells. These mitochondria populations are dynamic and undergo fusion and fission events. This activity serves to maintain the population in a healthy state. In the event of mitochondrial damage, sub-domains, or indeed whole mitochondria, can be degraded and population homeostasis achieved. If this process is overwhelmed cell death may result. Death of photoreceptors contributes to loss of vision in aging individuals and is associated with many eye diseases. In this study we used serial block face scanning electron microscopy of adult Macaca fascicularis retinae to examine the 3D structure of mitochondria in rod and cone photoreceptors. We show that healthy-looking photoreceptors contain mitochondria exhibiting a range of shapes which are associated with different regions of the cell. In some photoreceptors we observe mitochondrial swelling and other changes often associated with cellular stress. In rods and cones that appear stressed we identify elongated domains of mitochondria with densely-packed normal cristae associated with photoreceptor ciliary rootlet bundles. We observe mitochondrial fission and mitochondrion fragments localised to these domains. Swollen mitochondria with few intact cristae are located towards the periphery of the photoreceptor inner-segment in rods, whilst they are found throughout the cell in cones. Swollen mitochondria exhibit sites on the mitochondrial inner membrane which have undergone complex invagination resulting in membranous, electron-dense aggregates. Membrane contact occurs between the mitochondrion and the photoreceptor plasma membrane in the vicinity of these aggregates, and a series of subsequent membrane fusions results in expulsion of the mitochondrial aggregate from the photoreceptor. These events are primarily associated with rods. The potential fate of this purged material and consequences of its clearance by retinal pigment epithelia are discussed.
Separation and feature extraction of micro‐motion signal of ballistic target
Authors: Li Yuxi , Feng Cunqian , Xu Xuguang , Han Lixun , Wang Dayan 
Published: September 2021

AbstractDuring the process of the ballistic target's mid‐flight, it is very important to accurately identify the target. The separation of target micro‐Doppler curves and the extraction of characteristic parameters are the key to accurate identification. Aiming at the slow convergence speed of the traditional Fast‐ICA algorithm, this paper proposes an improved Fast‐ICA algorithm, which realizes the separation of the micro‐Doppler curve of the scattering points. On this basis, the trajectory of scattering points in space is analyzed, the expression of the target feature value is established, and the target feature parameters are effectively extracted. According to the results of the simulation experiment, the algorithm can achieve better signal separation and the characteristic parameters of the target are effectively extracted.
Media portrayals of the Hong Kong Occupy Central Movement’s social actors
Authors: Ho Janet , Chiu Ming Ming 
Published: September 2021

Abstract
We investigated how two English-language newspapers – Hong Kong’s South China Morning Post (SCMP) and the mainland China Daily (CD) – portrayed key social actors (police, students, protesters, and governments) during the Occupy Central/Yellow Umbrella movement. We examined emotional valence, arousal, and dominance characterizations in 1,180 news articles via a multilevel, multivariate outcome regression and critical discourse analysis. The findings reveal that emotional sentiments associated with students and protesters in SCMP were generally more positive than in CD but that this was reversed for the police and the government. Whereas SCMP deployed personal stories to construct a humanized image of protesters and students, CD relied on expert authority, rhetorical questions, and imagined scenarios to convey empathy towards Hong Kong residents, creating a villainized image of protesters. Our mixed-methods approach reveals how SCMP and CD portrayed students differently via the discursive frames of “optimistic dreamers” and “powerless scapegoats,” respectively.
The role of the body in descriptions of emotions
Authors: Ponsonnet Maïa , Laginha Kitty-Jean 
Published: September 2021

AbstractThis article presents the first systematic typological study of emotional expressions involving body parts at the scale of a continent, namely the Australian continent. The role of body parts in figurative descriptions of emotions, a well-established phenomenon across the world, is known to be widespread in Australian languages. This article presents a typology of body-based emotional expressions across a balanced sample of 67 languages, where we found that at least 30 distinct body parts occur in emotional expressions. The belly is by far the most frequent, and a dozen others also have significant representation. The study shows how the properties of these body parts – e.g., whether they are internal organs or visible facial parts – partly determine which historical scenarios led to their linguistic associations with emotions, and in turn, their semantic and figurative properties.