bluuebunny/crossref_metadata_2025_split huggingface dataset:
Completely dechlorinating of trichloroethene by a Dehalococcoides mccartyi-containing microbial consortium in the absence of cobalamin
Authors: Li Haijun , Wei Shanming , Liu Na , Du Yalu , Ding Guantao 
Published: November 2021

Abstract
Completely dechlorinating of trichloroethene (TCE) by Dehalococcoides mccartyi (D.mccartyi) is catalyzed by reductive dehalogenases (RDases) which possess cobalamin as the crucial cofactor, whereas virtually all pure D.mccartyi strains isolated thus far are corrinoid auxotrophs. Exogenous addition of commercially available cobalamin for real TCE-contaminated site decontamination is deemed to be unrealistic. In this study, TCE reduction by a D.mccartyi-containing microbial consortium utilizing biosynthetic cobalamin generated by interior corrinoid-producing organisms within this mixed consortia was studied. The results confirmed that subcultures with exogenous cobalamin omitting from the medium apparently were impervious and enabled to successively metabolize TCE to non-chlorinated ethene. The 2-bromoethanesulfonate and ampicillin resistance tests results suggested that bacteria (particularly certain ampicillin-sensitive ones) rather than methanogenic archaea within this microbial consortium were responsible for biosynthesizing cobalamin. Moreover, relative stable Ɛ-carbon values of TCE among treatments in disregard of whether exogenous cobalamin or selective inhibitors were existed in the medium also speculated that cobalamin biosynthesized by these organisms was enable to uptake and utilize by D.mccartyi for RDases synthesis and eventually participated in TCE reduction. Finally, the Illumina MiSeq sequencing analysis indicated that Desulfitobacterium and Acetobacterium in this microbial consortium probably both were in charge of de novo cobalamin biosynthesis to fulfillment the requirements of D.mccartyi for TCE metabolism.
Low-Dose Continuation of Lenvatinib for the Treatment of the Patients with Unresectable Thyroid Carcinoma
Authors: Murakami Daizo , Nishimoto Kohei , Takao Soshi , Miyamaru Satoru , Kadowaki Tomoka , Saito Haruki , Takeda Hiroki , Ise Momoko , Suyama Koichi , Orita Yorihisa 
Published: November 2021

Abstract
Background

Lenvatinib, a multi-tyrosine kinase inhibitor (TKI), has been proven to be an effective treatment option for patients with unresectable thyroid carcinoma. However, it causes certain adverse effects (AEs), and discontinuation or dose reduction of the drug is almost unavoidable. Few studies have investigated the impact of the length, dose, or interruption of lenvatinib administration on the prognosis of patients with unresectable thyroid carcinoma. Here, we aimed to verify the significance of our efforts to continue lenvatinib therapy to the longest duration possible with a reasonable daily dose and minimum discontinuation period in patients with unresectable thyroid carcinoma.
Methods

The clinical records of 42 patients with unresectable thyroid carcinoma treated with lenvatinib between 2015 and 2020 were retrospectively studied.
Results

The Cox proportional hazard model-based analysis indicated that the overall survival (OS) of patients treated with a mean dose of lenvatinib <8 mg/day was significantly better than that of those treated with 8-24 mg/day (hazard ratio (HR)=0.38, 95% confidence interval (CI): 0.03-4.99 for 1.14-4.54 mg/day, and HR=0.01, 95% CI: 0.00-0.13 for 4.56-7.97 mg/day) adjusted for various factors (sex, age, length of drug interruption, and so on). The cumulative administration dose of lenvatinib tended to be higher in patients treated with low doses (<8 mg/day) than in those treated with relatively high doses (8-24 mg/day).
Conclusion

Considering its AEs, continuation of lenvatinib administration with an adequate daily dose with drug interruption may be important for prolonging survival of patients with unresectable thyroid carcinoma.
Clinical Profiles in Acute Heart Failure
Authors: García-Gutiérrez Susana , Antón-Ladislao Ane , Quiros Raul , Lara Antonio , Rilo Irene , Rodriguez Ibon , Murga Nekane , Aguirre Urko , Quintana José M 
Published: November 2021

Abstract
Background:Phenotypes could be more frequently related to outcomes than classical classifications of AHF. Our goal was to identify clinical profiles for acute heart failure (AHF) based on clinical variables at the time of the patient arrival to the Emergency Department (ED).Methods:Design: Prospective cohort study. Participants: Patients with symptoms of AHF were recruited at the EDs of seven Spanish National Health Service hospitals between April 2013 and December 2014. Main measures: Information on sociodemographic, baseline functional status, medical history, and time since diagnosis was collected when the patient arrived at the ED. In addition, the MLWHF questionnaire was administered at arrival and at 1 year after discharge from the ED. Change in MLWHF score and mortality, revisits and readmissions during this first year were considered as outcomes. We combined multiple correspondence analysis (MCA) and cluster analysis (CA) to create clinical profiles.Results:A total of 1599 subjects were included in the study. Patients were located on two axes: one was defined as duration of HF and the other as cardiovascular comorbidity. Cluster analysis identified three subtypes of patient (A, B, and C), group B being most frequently corresponding to a “de novo” case. Differences in outcome, including mortality, readmissions and changes in MLWHF score, were encountered between group B and the rest of the groups, results being similar in groups A and C.Conclusions:The clinical phenotypes found were associated with clinical and patient-reported outcomes. Such clinical phenotypes could be useful in decision making processes in ED settings.Clinical registration:ClinicalTrials.gov ID: NCT03512704
Mapping Risks Associated with Soil Copper Contamination Using Availability and Bio-Availability Proxies at the European Scale
Authors: Sereni Laura , Guenet Bertrand , Lamy Isabelle 
Published: November 2021

Abstract
Soil contamination by trace elements like copper (Cu) can affect soil functioning. Environmental policies with guidelines and soil survey measurements still refer to the total contents of Cu in soils. However, Cu contents in soil solution or free Cu contents have been shown to be better proxies of risks of Cu mobility or (bio)availability for soil organisms. Several empirical equations have been defined at the local scale to predict the amounts of Cu in soil solution based on both total soil Cu contents and main soil parameters involved in the soil/solution partitioning. Nevertheless, despite the relevance for risk assessment, these equations are not applied at a large spatial scale due to difficulties to perform changes from local to regional. To progress in this challenge, we collected several empirical equations from literature and selected those allowing estimation of the amount of Cu in solution, used as a proxy of available Cu, from the knowledge of both total soil Cu contents and soil parameters. We did the same for the estimation of free Cu in solution, used as a proxy of bioavailable Cu These equations were used to provide European maps of (bio-)available Cu based on the one of total soil Cu over Europe. Results allowed comparing the maps of available and bio-available Cu at the European scale. This was done with respective median values of each form of Cu to identify specific areas of risks linked to these two proxies. Higher discrepancies were highlighted between the map of bioavailable Cu and the map of soil total Cu compared to the Cu available map. Such results can be used to assess environmental-related issues for land use planning.
Moderate Exercise Protects Against Joint Disease in a Murine Model of Osteoarthritis
Authors: Huesa Carmen , Dunning Lynette , MacDougal Kayleigh , Fegen Margaret , Ortiz Ana , McCulloch Kendall , McGrath Sarah , Litherland Gary J. , Crilly Anne , Ferrell William R. , Lockhart John C. , Goodyear Carl S. , Hof Rob van 't 
Published: November 2021

Abstract
Exercise is recommended as a non-pharmacological therapy for osteoarthritis (OA). Clinical studies investigating the impact of exercise on OA have primarily focussed on the assessment of joint pain and mobilisation, where positive outcomes have been demonstrated. Clinical imaging studies provide limited information on the impact of exercise (positive or negative) on the actual bone and soft tissue pathology. Various exercise regimes, with differing intensities and duration, have been used in a range of OA models, with disparate results. The present study provides definitive insight into the effect of moderate exercise on early joint pathology in the destabilisation of the medial meniscus (DMM) mouse model of OA. Exercise was induced by forced treadmill walking for 3 or 7 weeks. Joints were analysed by microcomputed tomography and histology. Exercise offered protection against cartilage damage and joint inflammation, and a temporary protection against osteosclerosis. Furthermore, exercise modified the metaphyseal trabecular microarchitecture of the osteoarthritic leg. Collectively, our findings provide scientific support for the clinical recommendation of moderate exercise as a physical therapy in OA. In addition to indirect benefit via positive physiological effects of weight loss, our data suggest direct short-term benefits in ameliorating pathology of cartilage, synovitis and bone.
Performance Mechanism of Hydrogel for Enhanced Oil Recovery: A Numerical Simulation Study Based on the Phase-Field Approach
Authors: Hayatolgheibi Seyed Hosein , Ameli Forough , Moghbeli Mohammad Reza 
Published: November 2021

Abstract
Hydrogels are increasingly applied in oil recovery processes. This leads to more controlled flow of fluids in porous media. In this process, hydrogel is injected to the reservoir to block the high permeability areas. The trapped oil in low permeability regions, is then swept by water flooding. pH-sensitive hydrogel microspheres were synthesized in another work of the authors, which effectively increased the oil recovery factor in experimental studies. In this communication, phase-field approach was used to simulate this process and to obtain the tuning parameters of the model including thickness of the contact surface(є), phase transform parameter(M0), excess free energy(\(\wedge\)), and interfacial tension. Diffusion of hydrogels was studied by Cahn–Hilliard conservative approach and the breakage, deformation, and plugging mechanisms were analyzed, based on pressure drop variations in micromodel. Moreover, Effective parameters on oil recovery factor were analyzed. Results indicated a good agreement between experimental and modeling studies of oil recovery factor in water and hydrogel flooding with absolute errors of 2.29% and 4.06%, respectively. The recovery factor was calculated using a statistical method which was in good agreement with the modeling results. The tuned parameters of the model were reported as, є= 111.7µm, M0= 5*10-13m3/s, \(\wedge\)=-0.0003 J/m3, and δ = 2×10-5 Pa.s.
Pretreated Sugarcane Bagasse Result in More Efficient Degradation by Streptomyces sp S2
Authors: Agung Stanislaus Aditya , Yanto Dede Heri Yuli , Meryandini Anja , Sunarti Titi Candra 
Published: November 2021

Abstract
Streptomyces genera plays important role in lignocellulose degradation. Many research founds Streptomyces has cellulolytic and ligninolytic enzymes that sufficient to degrade lignocellulosic materials. However, minimum lignocellulosic material condition that can efficiently degraded by Streptomyces sp. has not been fully understood. In this research, three pretreament conditions (physical, alkaline-hydrotermal, and hydrogen-peroxide chemical treatments) of sugarcane bagasse used as lignocellulosic material, to further degraded by Streptomyces sp. S2. Lignocellulose component measurement conclude that raw (physical treated only) bagasse wasn’t efficiently degraded by Streptomyces sp S2. Hydrogen-peroxide was effective on reducing both syringil and guaiacyl lignin, meanwhile alkaline-hydrotermal pretreatment was very effective on reducing syringil lignin. This study suggest that hydrogen-peroxide pretreatment can be used in many type of lignocellulosic material, which can be further degraded by Streptomyces sp. S2. Alkaline-hydrotermal preteatment on the other hand is best suited to degrade lignocellulosic material that have high percentage of syringil lignin.
Pseudomonas Species Prevalence, Protein Analysis, and Antibiotic Resistance: An Evolving Public Health Challenge
Authors: Elbehiry Ayman , Marzouk Eman , Moussa Ihab , Abalkhail Adil , Ibrahim Mai , Hamada Mohamed , Rawway Mohammed 
Published: November 2021

Abstract
Psychrotrophic Pseudomonas is one of the significant microbes that lead to putrefaction in chilled meat. One of the biggest problems in the detection of Pseudomonas is that several species are seemingly identical. Antibiotic resistance is an alternative considerable challenge worldwide. Therefore, this study was designed to apply an accurate technique for the discrepancy of Pseudomonas species and to study their resistance against various antimicrobials. A total of 320 chicken meat specimens were cultivated, and phenotypically recognized the isolated bacteria. Protein analysis was carried out for cultured isolates via Microflex LT. The resistance of Pseudomonas isolates was recorded through Vitek®ฏ 2 AST-GN83 cards. Overall, 69 samples were identified as Pseudomonas spp. and included 18 Pseudomonas lundensis (P. lundensis), 16 Pseudomonas fragi (P. fragi), 13 Pseudomonas oryzihabitans (P. oryzihabitans), 10 Pseudomonas stutzeri (P. stutzeri), 5 Pseudomonas fluorescens (P. fluorescens), 4 Pseudomonas putida (P. putida), and 3 Pseudomonas aeruginosa (P. aeruginosa) isolates. Microflex LT identified all Pseudomonas isolates (100%) correctly with a score value ≥2.00. PCA positively discriminated the identified isolates into various groups. The antimicrobial resistance levels against Pseudomonas isolates were 81.16% for nitrofurantoin, 71% for ampicillin and ampicillin/sulbactam, 65.22% for cefuroxime and ceftriaxone, 55% for aztreonam, and 49.28% for ciprofloxacin. The susceptibilities were 100% for cefotaxime, 98.55% for ceftazidime, 94.20% for each piperacillin/tazobactam and cefepime, 91.3% for cefazolin. In conclusion, chicken meat was found to be contaminated with different Pseudomonas spp., with high incidence rates of P. lundensis. Microflex LT is a potent tool for distinguishing Pseudomonads at the species level.
The Relationship Between Sleep Quality and Neck Pain, Shoulder Pain and Disability, Physical Activity, and Health Perception Among Middle-Aged Women : A Cross-Sectional Study
Authors: Lee Myung Kyung , Oh Jihyun 
Published: November 2021

Abstract
Background

Sleep quality is an important physical requirement for a healthy life, and good sleep quality has been recognized as a significant component in physical and mental health and well-being. The purpose of this study was to identify the factors that affect sleep quality as well as the relationship between sleep quality and neck pain, shoulder pain and disability, physical activity, and health perception.
Methods

We conducted surveys on 494 women between the age of 35 and 64 years. The study evaluated neck pain, shoulder pain and disability, physical activity, self-health perception and sleep quality with self-reported questionnaires in middle-aged women. Data were analyzed using SPSS 23.0.
Results

The results showed that the more severe the neck pain and shoulder pain and disability, the worse the sleep quality was in middle-aged women and the better the health perception, the lower the sleep quality score was, indicating good sleep quality. Shoulder pain, self-perceived task difficulty, and health perception were identified as variables that affected the sleep quality in middle-aged women. The explanatory power of the model in explaining sleep quality was 22.9%.
Conclusions

To improve the sleep quality in middle-aged women, healthcare workers should take into consideration the subjects’ symptoms, such as pain, in accordance to their health. Perceived difficulty of one’s work and their health can affect sleep quality; hence, it is necessary to develop health intervention strategy to provide health management, emotional support, and social support for the purpose of improving daily sleep quality.
Genetic Characterization of Global Cultivated Potato Clones, Including Korean Potatoes, Using Genome-Wide Single Nucleotide Polymorphism Markers
Authors: Jo Kwang Ryong , Cho Seungho , Cho Ji-Hong , Park Hyun-Jin , Choi Jang-Gyu , Park Young-Eun , Cho Kwang-Soo 
Published: November 2021

Abstract
Characterizing the genetic diversity and population structure of breeding materials is essential for breeding to improve crop plants. The potato is an important non-cereal food crop worldwide, but breeding potatoes remains challenging owing to their auto-tetraploidy and highly heterozygous genome. We evaluated the genetic structure of a 110-line Korean potato germplasm using the SolCAP 8303 single nucleotide polymorphism (SNP) Infinium array and compared it with potato clones from other countries to understand the genetic landscape of cultivated potatoes. Following the tetraploid model, we conducted population structure analysis, revealing three subpopulations represented by two Korean potato groups and one separate foreign potato group within 110 lines. When analyzing 393 global potato clones, country/region-specific genetic patterns were revealed. The Korean potato clones exhibited higher heterozygosity than those from Japan, the United States, and other potato landraces. We also employed integrated extended haplotype homozygosity (iHS) and cross-population extended haplotype homozygosity (XP-EHH) to identify selection signatures spanning candidate genes associated with biotic and abiotic stress tolerance. Based on the informativeness of SNPs for dosage genotyping calls, 10 highly informative SNPs discriminating all 393 potatoes were identified. Our results could help understanding a potato breeding history that reflects regional adaptations and distinct market demands.
Hypoxia promotes a perinatal-like progenitor state in the adult murine epicardium
Authors: Sayed Angeliqua , Turoczi Szimonetta , Soares-da-Silva Francisca , Marazzi Giovanna , Hulot Jean-Sebastien , Sassoon David , Valente Mariana 
Published: November 2021

Abstract
The epicardium is a reservoir of progenitors that give rise to coronary vasculature and stroma during development and mediates cardiac vascular repair in lower vertebrates. However, its role as a source of progenitors in the adult mammalian heart remains unclear due to lack of clear lineage markers and single-cell culture systems to elucidate epicardial progeny cell fate. We found that in vivo exposure of mice to physiological hypoxia induced adult epicardial cells to re-enter the cell cycle and to express a subset of developmental genes. Multiplex single cell transcriptional profiling revealed a lineage relationship between epicardial cells and smooth muscle, stromal, and endothelial fates, and that physiological hypoxia promoted an endothelial cell fate. In vitro clonal analyses of purified epicardial cells showed that cell growth and subsequent differentiation is dependent upon hypoxia, and that resident epicardial cells retain progenitor identity in the adult mammalian heart with self-renewal and multilineage differentiation potential. These results point to a source of progenitor cells in the adult heart that can promote heart revascularization, providing an invaluable in vitro model for further studies.
Standardized Stimulation Protocols Are Needed for Comparable Analysis of Pain-related Evoked Potentials (PREP)
Authors: Brech Katharina , Enax-Krumova Elena K. , Eitner Lynn , Vollert Jan , Maier Christoph , Höffken Oliver , Tegenthoff Martin , Özgül Özüm Simal 
Published: November 2021

Abstract
Objective: Pain-related evoked potentials (PREP) are increasingly used to investigate nociception and small-fibre function. Due to lack of a standard stimulation protocol, it is unclear whether results from studies using different protocols are comparable. Aim of the study was to assess the influence of different stimulation parameters on N1P1-amplitudes, N1-latencies and PREP-induced pain intensity. Methods: In a cross-over design we examined 31 healthy volunteers using four different stimulation protocols (number of stimulation electrodes 1 vs. 3, stimulus durations 200 µs vs. 500 µs) in a randomized order. Statistics: paired t-test, ANOVA, correlation analyses. Results: Longer stimulus duration induced higher N1P1-amplitudes (p<0.05) and higher pain intensity (p<0.001). Stimulation with 3 electrodes lead to a lower pain intensity (p<0.01), whereas the N1P1-amplitude and stimulus intensity at twofold of individual pain remained unaffected by the number of electrodes. Also, there was no relation between stimulus intensities and N1P1-amplitudes (one electrode: r=0.079; p=0.646, three electrodes: r=-0.10, p=0.70) was observed. N1-latencies remained comparable between the four protocols.Conclusions and Significance: The use of different stimulation protocols for PREP is limited by relevant differences in the N1P1-amplitudes and evoked pain intensities. Standard consented stimulation protocols are needed to allow data comparison between different labs and studies.
Asymmetrical Attention to Gambling-related Stimuli and Pupillary Dilation in Response to Wins and Losses in Problem Gamblers
Authors: Shigemune Yayoi , Midorikawa Akira 
Published: November 2021

Abstract
Gambling disorder and problem gambling are characterized by persistent and repetitive problematic gambling behavior. Attentional bias toward gambling-related stimuli such as casino chips, dice, roulette, etc. have been observed in problem gamblers (PGs), but it remains unclear whether stimuli in gambling tasks elicit greater attention and pupillary responses in PGs. To address this issue, we administrated PGs and non-problem gamblers (NPGs) a gambling task accompanied by eye-tracking measurements, in which the participants were required to choose one of the paired pictures to receive monetary rewards and avoid punishments. Concerning attentional allocation, PG showed a greater attentional preference for the right-hand pictures in the decision and feedback phases, and compared to NPGs, PGs’ attention was narrower and more focused on the left-hand pictures in the decision phase. Concerning pupillary dynamics indicative of noradrenergic locus coeruleus activity, pupillary dilation in response to rewards and punishments was observed only in PGs. Studies of unilateral spatial neglect have proposed that asymmetric attention is induced by hemispheric imbalance. Accordingly, asymmetrical allocation of attention by PGs may reflect hemispheric imbalance, and pupillary dynamics may reflect sensitivity to wins and losses.