bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tooth Wear Evaluation System (TWES) 2.0—Reliability of diagnosis with and without computer‐assisted evaluation
Authors: Roehl Jakob C. , Jakstat Holger A. , Becker Kai , Wetselaar Peter , Ahlers M. Oliver 
Published: October 2021

AbstractBackgroundTooth wear is a multifactorial process, leading to the loss of dental hard tissues. Therefore, it is important to detect the level of tooth wear at an early stage, so monitoring can be initiated. The Tooth Wear Evaluation System (TWES) enables such a multistage diagnosis of tooth wear. The further developed TWES 2.0 contains a complete taxonomy of tooth wear, but its reliability has not yet been validated.ObjectivesThe aim of the study was to examine in a randomised controlled trial (RCT) whether diagnoses made based on the TWES 2.0 are reproducible and whether this reproducibility is also achieved with computer‐assisted diagnostics.Methods44 dental students received extensive training in TWES 2.0 assessment and taxonomy. The students each evaluated at least 10 (of the present 14) anonymised patient cases using gypsum models and high‐resolution intra‐oral photographs according to TWES 2.0. One half initially evaluated on paper; the other half used dedicated software (CMDfact / CMDbrux). After half of the patient cases (5), the evaluation methods were switched (AB/BA crossover design). The diagnoses were then evaluated for agreement with the predefined sample solution.ResultsEvaluation of agreement with the sample solution according to Cohen's kappa indicated a value of 0.46 for manual (traditional) evaluation; and 0.44 for computer‐assisted evaluation. Evaluation of agreement between examiners was 0.38 for manual and 0.48 for computer‐assisted evaluation (Fleiss’ kappa).ConclusionThe results of this study proved that the taxonomy of the TWES 2.0 has acceptable reliability and can thus be used by dentists. Accordingly, the system can be learned quickly even by untrained practitioners. Comparable results are achieved with computer‐assisted evaluation.
Water Quality Monitoring in Inle Lake, Myanmar from the Floating Garden Activity
Authors: Khaung Theingi , Iwai Chuleemas Boonthai , Chuasavathi Thammared , Chuasavathi Thammared 
Published: October 2021

Inle Lake is the second largest inland lake in Myanmar. Floating gardens, mostly for tomato cultivation, are a unique and profitable method of agriculture used by people living on and around the lake. This study investigated the water quality of Inle Lake and how it has been affected by the different agricultural practices used in tomato cultivation on floating garden beds, by measuring pollution levels. Water samples were collected from the sites representing two types of agricultural practice from four villages. The first was designated as being grown under good agricultural practices (GAP), and the other as under non-good agricultural practices (non-GAP), with this study undertaken during wet season, 2019 and dry season, 2020. Two additional sets of water samples were collected as references. One of these sets was from the center of the lake and the other from an inlet stream to the lake. All water samples were analyzed for physical and chemical properties. The results found that Ca2+, Mg2+ and HCO3- were dominant in the lake surface water. The results showed significant differences in the mean values for some water quality parameters between the GAP and non-GAP of each study sites in both seasons. In particular, nutrient pollution from chemicals such as nitrogen and phosphorus from the non-GAP were significantly higher than those from GAP. Water quality index was calculated to describe the overall quality of lake surface water. It was observed that the water quality was almost threatened in the floating garden areas. In comparison between two practices, the non-GAP gave the higher water quality index value than the GAP. This investigated that poor management of fertilizers usage has had a negative effect on the water quality of the lake. The differences seen in water quality from the GAP and non-GAP areas, point to ways to successfully manage sources of water pollution in order to better conserve the lake by sustainable agricultural production.
Anisotropic solution for compact star in 5D Einstein–Gauss–Bonnet gravity
Authors: Maurya S. K. , Pradhan Anirudh , Banerjee Ayan , Tello-Ortiz Francisco , Jasim M. K. 
Published: October 2021

In astronomy, the study of compact stellar remnants — white dwarfs, neutron stars, black holes — has attracted much attention for addressing fundamental principles of physics under extreme conditions in the core of compact objects. In a recent argument, Maurya et al. [Eur. Phys. J. C 77, 45 (2017)] have proposed an exact solution depending on a specific spacetime geometry. Here, we construct equilibrium configurations of compact stars for the same spacetime that make it interesting for modeling high density physical astronomical objects. All calculations are carried out within the framework of the five-dimensional Einstein–Gauss–Bonnet gravity. Our main interest is to explore the dependence of the physical properties of these compact stars depending on the Gauss–Bonnet coupling constant. The interior solutions have been matched to an exterior Boulware–Deser solution for [Formula: see text] spacetime. Our finding ensures that all energy conditions hold, and the speed of sound remains causal, everywhere inside the star. Moreover, we study the dynamical stability of stellar structure by taking into account the modified field equations using the theory of adiabatic radial oscillations developed by Chandrasekhar. Based on the observational data for radii and masses coming from different astronomical sources, we show that our model is compatible and physically relevant.
Study on Mechanism of Jiawei Chaiqin Wendan Decoction in Treatment of Vestibular Migraine Based on Network Pharmacology and Molecular Docking Technology
Authors: Liu Menglin , Fan Genhao , Zhang Daopei , Zhu Mingjun , Zhang Huailiang 
Published: October 2021

Objective. To predict the main active ingredients, potential targets, and key pathways of Jiawei Chaiqin Wendan decoction treatment in vestibular migraine and explore possible mechanisms by network pharmacology and molecular docking technology. Methods. The active ingredients and related targets of Jiawei Chaiqin Wendan decoction were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The corresponding genes of the target were queried by UniProt database, and the “drug-compound-target-disease” network was constructed by Cytoscape 3.7.2 software. GO functional enrichment analysis and KEGG pathway enrichment analysis were carried out by R software and Bioconductor, and column chart and bubble chart were drawn by Prism software and OmicShare database for visualization. Finally, the mechanism and potential targets of Jiawei Chaiqin Wendan decoction in the treatment of vestibular migraine were predicted. Results. The “drug-compound-target-disease” network contains 154 active ingredients and 85 intersection targets. The key targets include AKT1, IL6, MAPK3, VEGFA, EGFR, CASP3, EGF, MAPK1, PTGS2, and ESR1. A total of 1939 items were obtained by GO functional enrichment analysis (

P

< 0.05). KEGG pathway enrichment analysis screened 156 signal pathways (

P

< 0.05), involving PI3K-Akt signal pathway, AGE-RAGE signal pathway in diabetes complications, MAPK signal pathway, HIF-1 signal pathway, IL-17 signal pathway, etc. Molecular docking results showed that quercetin, luteolin, kaempferol, tanshinone IIa, wogonin, naringenin, nobiletin, dihydrotanshinlactone, beta-sitosterol, and salviolone have good affinity with core target proteins IL6, PTGS2, MAPK1, MAPK3, and CGRP1. Conclusion. The active ingredients in Jiawei Chaiqin Wendan decoction may regulate the levels of inflammatory factors and neurotransmitters by acting on multiple targets such as IL6, MAPK3, MAPK1, and PTGS2, so as to play a therapeutic role in vestibular migraine.
Novel cholesterol efflux assay using immobilized liposome-bound gel beads: Confirmation and improvement for application in clinical laboratory
Authors: Horiuchi Yuna , Lai Shao-Jui , Kameda Takahiro , Tozuka Minoru , Ohkawa Ryunosuke 
Published: October 2021

Objectives Cholesterol efflux capacity (CEC), an atheroprotective function of high-density lipoprotein, is expected to be a potential biomarker for cardiovascular disease. However, CEC has not been widely introduced for application in clinical laboratories because of the complexity of the conventional CEC assay using cells and radioactive materials. Previously, we developed a novel CEC assay using immobilized liposome-bound gel beads (ILG), which solves these issues. We aimed to confirm the validation and further improve the ILG method for application in the clinical setting. Methods Cholesterol efflux capacity values by the ILG method assayed for shorter incubation time (4 h) were compared to those assayed for 16 h (our previous ILG method). To investigate a reference material that can correct the variation between ILG manufacturing lots, bovine serum albumin, human gamma-globulins, and globulin complexes were evaluated. CEC values were also estimated in plasmas obtained with different anticoagulants, serum treated with freeze-thaw cycles, and serum mixed with several interference substances. Results The CEC of 4- and 16-h incubation times were well correlated. Globulin complexes may be used as a reference material. Plasma can be used as the specimen. The serum and stored temperature of the specimen did not largely affect CEC. Hemoglobin and chyle did not have an effect on CEC, whereas high-bilirubin serum showed elevated CEC. The effect of bilirubin was nearly canceled by subtracting basal fluorescence intensity. Conclusions Present ILG method further fulfills some requirements for application in clinical laboratory. Using this reliable simple method, evaluation for clinical significance of CEC is expected.
Patient-reported outcome measures in systemic lupus erythematosus by a web-based application: A randomized, crossover, agreement study
Authors: Uhrenholt Line , Høstgaard Simone , Pedersen Julie F , Christensen Robin , Dreyer Lene , Leffers Henrik C B , Taylor Peter C , Strand Vibeke , Jacobsen Søren , Voss Anne , Gregersen Jon W , Kristensen Salome 
Published: October 2021

Objectives Patient-reported outcome measures (PROMs) are evaluated in randomized controlled trials (RCTs) in patients with systemic lupus erythematosus (SLE), but not widely used in clinical practice. However, interest in incorporating PROMs into the management of SLE is increasing as PROMs provide a unique insight into the patient’s perception of lupus disease activity. The objective was to assess agreement in PROMs answered using a web app versus an outpatient touchscreen among patients with SLE. Methods In a crossover RCT, SLE patients answered the following PROMs in a random order using the web app and the outpatient touchscreen: Systemic Lupus Erythematosus Activity Questionnaire (SLAQ) Global Health, SLAQ Symptom, SLAQ Total, SLAQ Worsening, Pain Visual Analog Scale (VAS), Fatigue VAS, Patient Global Health VAS, Health Assessment Questionnaire Disability Index (HAQ-DI), Patient Acceptable Symptom State (PASS), and an Anchoring Question. Equivalence between the two device types was demonstrated if the 95% confidence interval (95% CI) of the difference in PROM scores was within the prespecified equivalence margin. Agreement between the two device types was assessed using mixed linear models. Results Thirty-four patients with SLE were included. Equivalence was demonstrated between the two device types for SLAQ Global Health with a difference of −0.21 (95% CI: −0.65 to 0.23). Moreover, equivalence was also found for HAQ-DI, Pain VAS, and Fatigue VAS whereas only comparability within the limits of the Minimal Clinically Important Difference (MCID) was demonstrated for VAS Patient Global Health. Statistical comparability was demonstrated for SLAQ Total, SLAQ Worsening, PASS, and Anchoring Question (no predefined MCID/equivalence margins available). However, a statistically significant difference between device types was observed for the SLAQ Symptom of −0.56 (95% CI: −1.10 to −0.01). The difference was, however, very small when considering the scale range of 0–24; thus, it was not judged to be of clinical relevance. Preference for the web app was very high (91.2%). Conclusion For the first time ever, equivalence and comparability between two electronic device types for various PROMs were demonstrated among patients with SLE. Implementation of the device is expected to improve the management of SLE.
The effects of inhaling hydrogen gas on macrophage polarization, fibrosis, and lung function in mice with bleomycin-induced lung injury
Authors: Aokage Toshiyuki , Seya Mizuki , Hirayama Takahiro , Nojima Tsuyoshi , Iketani Masumi , Ishikawa Michiko , Terasaki Yasuhiro , Taniguchi Akihiko , Miyahara Nobuaki , Nakao Atsunori , Ohsawa Ikuroh , Naito Hiromichi 
Published: October 2021

Abstract
Background
Acute respiratory distress syndrome, which is caused by acute lung injury, is a destructive respiratory disorder caused by a systemic inflammatory response. Persistent inflammation results in irreversible alveolar fibrosis. Because hydrogen gas possesses anti-inflammatory properties, we hypothesized that daily repeated inhalation of hydrogen gas could suppress persistent lung inflammation by inducing functional changes in macrophages, and consequently inhibit lung fibrosis during late-phase lung injury.

Methods
To test this hypothesis, lung injury was induced in mice by intratracheal administration of bleomycin (1.0 mg/kg). Mice were exposed to control gas (air) or hydrogen (3.2% in air) for 6 h every day for 7 or 21 days. Respiratory physiology, tissue pathology, markers of inflammation, and macrophage phenotypes were examined.

Results
Mice with bleomycin-induced lung injury that received daily hydrogen therapy for 21 days (BH group) exhibited higher static compliance (0.056 mL/cmH2O, 95% CI 0.047–0.064) than mice with bleomycin-induced lung injury exposed only to air (BA group; 0.042 mL/cmH2O, 95% CI 0.031–0.053, p = 0.02) and lower static elastance (BH 18.8 cmH2O/mL, [95% CI 15.4–22.2] vs. BA 26.7 cmH2O/mL [95% CI 19.6–33.8], p = 0.02). When the mRNA levels of pro-inflammatory cytokines were examined 7 days after bleomycin administration, interleukin (IL)-6, IL-4 and IL-13 were significantly lower in the BH group than in the BA group. There were significantly fewer M2-biased macrophages in the alveolar interstitium of the BH group than in the BA group (3.1% [95% CI 1.6–4.5%] vs. 1.1% [95% CI 0.3–1.8%], p = 0.008).

Conclusions
The results suggest that hydrogen inhalation inhibits the deterioration of respiratory physiological function and alveolar fibrosis in this model of lung injury.
Laparoscopic subtotal cholecystectomy after percutaneous transhepatic gallbladder drainage for grade II or III acute cholecystitis
Authors: Ie Masafumi , Katsura Morihiro , Kanda Yukihiro , Kato Takashi , Sunagawa Kazuya , Mototake Hidemitsu 
Published: October 2021

Abstract
Background
Severe adhesions and fibrosis between the posterior wall of the gallbladder and liver bed often render total cholecystectomy after percutaneous transhepatic gallbladder drainage (PTGBD) difficult, leading to high open conversion rates. Since the publication of Tokyo Guidelines 2018 (TG18), our policy has shifted from open conversion to subtotal cholecystectomy (SC) when total laparoscopic cholecystectomy for difficult cases of cholecystitis is not feasible. Recently, SC has been frequently applied as bailout surgery for complicated cholecystitis. Nonetheless, the efficacy and validity of laparoscopic SC after PTGBD remain unclear. This study aimed to evaluate the safety and feasibility of laparoscopic SC after PTGBD for grade II or III acute cholecystitis (AC) by comparing two periods of altered surgical strategies.

Methods
This retrospective cohort study was conducted between January 2013 and December 2020. A total of 44 eligible patients with grade II or III AC were divided according to the time of cholecystitis onset into the pre-TG18 group (2013–2017, n = 17) and post-TG18 group (2018–2020, n = 27). Patients’ background demographics, surgical method, surgical results, and postoperative complications were compared.

Results
The interval between PTGBD and surgery was significantly longer in the post-TG18 group than in the pre-TG18 group (15 [interquartile range: 9–42] days vs. 8 [4–11] days; P = 0.010). The frequency of laparoscopic cholecystectomy significantly increased from 52.9% in the pre-TG18 group to 88.9% in the post-TG18 group (P = 0.007), whereas the frequency of SC was 23.5% and 40.7%, respectively, which showed no statistically significant difference (P = 0.241). However, the rate of laparoscopic SC significantly increased from 0 to 90.9% among 15 SC cases, whereas the rate of open SC significantly plummeted from 100 to 9.1% (P = 0.001). Significant differences in the operative time, amount of intraoperative bleeding, and incidence of postoperative complications (wound infection and subhepatic abscess) were not observed. Mortality, bile leakage, and bile duct injury did not occur in either group.

Conclusions
For grade II or III AC after PTGBD, aggressive adoption of SC increased the completion rate of laparoscopic surgery. Laparoscopic SC is a safe and feasible treatment option.
Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications
Authors: Mital Suraj , Christie Graham , Dikicioglu Duygu 
Published: October 2021

AbstractRecombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details the methods and strategies researchers have utilized for expression of difficult to express (DtE), industrially relevant recombinant enzymes in E. coli expression strains. Our review identifies an absence of a coherent strategy with disparate practices being used to promote solubility. We discuss the potential to approach recombinant expression systematically, with the aid of modern bioinformatics, modelling, and ‘omics’ based systems-level analysis techniques to provide a structured, holistic approach. Our analysis also identifies potential gaps in the methods used to report metadata in publications and the impact on the reproducibility and growth of the research in this field.
A deep learning quantified stroma-immune score to predict survival of patients with stage II–III colorectal cancer
Authors: Xu Zeyan , Li Yong , Wang Yingyi , Zhang Shenyan , Huang Yanqi , Yao Su , Han Chu , Pan Xipeng , Shi Zhenwei , Mao Yun , Xu Yao , Huang Xiaomei , Lin Huan , Chen Xin , Liang Changhong , Li Zhenhui , Zhao Ke , Zhang Qingling , Liu Zaiyi 
Published: October 2021

Abstract
Background
Profound heterogeneity in prognosis has been observed in colorectal cancer (CRC) patients with intermediate levels of disease (stage II–III), advocating the identification of valuable biomarkers that could improve the prognostic stratification. This study aims to develop a deep learning-based pipeline for fully automatic quantification of immune infiltration within the stroma region on immunohistochemical (IHC) whole-slide images (WSIs) and further analyze its prognostic value in CRC.

Methods
Patients from two independent cohorts were divided into three groups: the development group (N = 200), the internal (N = 134), and the external validation group (N = 90). We trained a convolutional neural network for tissue classification of CD3 and CD8 stained WSIs. A scoring system, named stroma-immune score, was established by quantifying the density of CD3+ and CD8+ T-cells infiltration in the stroma region.

Results
Patients with higher stroma-immune scores had much longer survival. In the development group, 5-year survival rates of the low and high scores were 55.7% and 80.8% (hazard ratio [HR] for high vs. low 0.39, 95% confidence interval [CI] 0.24–0.63, P < 0.001). These results were confirmed in the internal and external validation groups with 5-year survival rates of low and high scores were 57.1% and 78.8%, 63.9% and 88.9%, respectively (internal: HR for high vs. low 0.49, 95% CI 0.28–0.88, P = 0.017; external: HR for high vs. low 0.35, 95% CI 0.15–0.83, P = 0.018). The combination of stroma-immune score and tumor-node-metastasis (TNM) stage showed better discrimination ability for survival prediction than using the TNM stage alone.

Conclusions
We proposed a stroma-immune score via a deep learning-based pipeline to quantify CD3+ and CD8+ T-cells densities within the stroma region on WSIs of CRC and further predict survival.
Nanomedicine for increasing the oral bioavailability of cancer treatments
Authors: Parodi Alessandro , Buzaeva Polina , Nigovora Daria , Baldin Alexey , Kostyushev Dmitry , Chulanov Vladimir , Savvateeva Lyudmila V. , Zamyatnin Andrey A. 
Published: October 2021

Abstract
Oral administration is an appealing route of delivering cancer treatments. However, the gastrointestinal tract is characterized by specific and efficient physical, chemical, and biological barriers that decrease the bioavailability of medications, including chemotherapeutics. In recent decades, the fields of material science and nanomedicine have generated several delivery platforms with high potential for overcoming multiple barriers associated to oral administration. This review describes the properties of several nanodelivery systems that improve the bioavailability of orally administered therapeutics, highlighting their advantages and disadvantages in generating successful anticancer oral nanomedicines.

Graphical Abstract
The Bridge Building Model: connecting evidence-based practice, evidence-based research, public involvement and needs led research
Authors: Ormstad Heidi , Jamtvedt Gro , Svege Ida , Crowe Sally 
Published: October 2021

AbstractThis paper describes a model developed by an interdisciplinary team of research and public engagement specialists, with backgrounds in health and social care research, higher education, evidence-based practice, leadership, commissioning research and public involvement and engagement. The model we propose combinesevidence-based practice,evidence-based research,public involvementandneeds led research. Our aim is to capitalise on the joining of the rationale and methods for these approaches, which have all been highlighted as important, but for which there has been a lack of models for integration. Our ambition is to argue for and show an effective and evidence-based way of working that bridges health and social care needs identification, evidence-based practice and research.
Obsessive–compulsive disorder post-COVID-19: a case presentation
Authors: Alkhamees Abdulmajeed A. 
Published: October 2021

Abstract
Background
Since the emergence of the COVID-19 pandemic and the significant changes that impacted because of it, people around the world have been left dealing with its consequences—fear of becoming ill and dying, fear of losing loved ones, uncertainty about the future, and imposed social isolation—several elements which could lead to psychological consequences. Moreover, as suggested by recent evidence, the virus acts as a factor in causing psychological symptoms, including depression, anxiety, fatigue, and post-traumatic stress disorder.

Case presentation
Here, we report a case of a patient with new-onset OCD after his recovery from the coronavirus disease, which presented in the form of recurrent and persistent intrusive thoughts and doubts which responded to medication.

Conclusions
This case shows the potential of COVID-19-associated inflammatory triggers to precipitate or induce obsessive–compulsive symptoms. Although this case cannot support causation, it does stress the bidirectional effects that physical and mental illness share.