bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copper-Iron Oxide: A Highly Effective Photocatalyst Than TiO2 Prepared by One-Step Sparking Process
Authors: Panthawan Arisara , Jumrus Nidchamon , Sanmuangmoon Panupong , Thongpan Winai , Kumpika Tewasin , Sro Wattikon , Kantarak Ekkapong , Tuantranont Adisorn , Singjai Pisith , Thongsuwan Wiradej 
Published: December 2021

Abstract
Copper-iron (Cu-Fe) oxide composite films were successfully deposited on quartz substrate by a facile sparking process. The nanoparticles were deposited on the substrate after sparking off the Fe and Cu tips with different ratios and were then annealed at different temperatures. The network particles was observed after annealed the film at 700°C. Meanwhile, XRD and SAED patterns of the annealed films at 700°C consisted of a mixed phase of CuO, γ-Fe2O3, CuFe2O4 and CuFe2O. The film with a lowest energy band gap (Eg) of 2.56 eV was observed after anneal at 700°C. Interestingly, the optimum ratio and annealing temperature show highly photocatalytic activity than annealed TiO2 at 500 and 700°C. This is a novel photocatalyst which can be replace TiO2 for photocatalytic applications in the future.
Directed Evolution of The PobR Allosteric Transcription Factor To Generate A Biosensor For 4-Hydroxymandelic Acid
Authors: Liang YaoYao , Luo Juan , Yang Chenhao , Guo Shuning , Zhang Bowen , Chen Fengqianrui , Su Kairui , Zhang Yulong , Dong Yi , Wang Zhihao , Fu Hongda , Sui Guangchao , Wang Pengchao 
Published: December 2021

Abstract
4-Hydroxymandelic acid (HMA) is widely applied in pharmaceuticals, food and cosmetics. In this study, we aimed to develop an allosteric transcription factors (aTFs) based biosensor for HMA. PobR, an aTF for HMA analog 4-hydroxybenzoic acid, was used to alter its selectivity and create novel aTFs responsive to HMA by directed evolution. We established a PobR mutant library with a capacity of 550,000 mutants using error-prone PCR and Megawhop PCR. Through our screening, two mutants were obtained with responsiveness to HMA. Analysis of each missense mutation indicating residues 122-126 were involved in its PobR ligand specificity. These results showed the effectiveness of directed evolution in switching the ligand specificity of a biosensor and improving HMA production.
A Bayesian Network Structure Learning Approach to Identify Genes Associated with Stress in Spleens of Chickens
Authors: Rodriguez E. A. Videla , Mitchell John B.O. , Smith V. Anne 
Published: December 2021

Abstract

Differences in the expression patterns of genes have been used to measure the effects of non-stress or stress conditions in poultry species. However, the list of genes identified can be extensive and they might be related to several biological systems. Therefore, the aim of this study was to identify a small set of genes closely associated with stress in a poultry animal model, the chicken (Gallus gallus), by reusing and combining data previously published together with bioinformatic analysis and Bayesian networks in a multi-step approach. Two datasets were collected from publicly available repositories and pre-processed. Bioinformatics analyses were performed to identify genes common to both datasets that showed differential expression patterns between non-stress and stress conditions. Bayesian networks were learnt using a Simulated Annealing algorithm implemented in the software Banjo. The structure of the Bayesian network consisted of 16 out of 19 genes in addition to the stress condition. CARD19 displayed a direct relationship with the stress condition, and three other genes, CYGB, BRAT1, and EPN3 were also relevant for the stress condition. The biological functionality of these genes are related to damage, apoptosis, and oxygen provision, and they could potentially be further explored as biomarkers of stress.
Efficacy of Fluralaner (Exzolt®) for the Treatment of Natural Allopsoroptoides Galli Infestations in Laying Hens
Authors: Soares Nilce Maria , Tucci Edna Clara , Perdoncini Gustavo , Junior Ricardo Della Matta 
Published: December 2021

Abstract
BackgroundMite infestations in laying hens can cause losses to producers due to stress, reduced egg production and even death of birds. A new species of mite, Allopsoroptoides galli (A. galli), Analgoidea: Psoroptoididae, was recently identified in commercial laying farms in Brazil, causing damage due to its highly aggressive infestation that results in a sharp drop in egg production and culling. The present study evaluated the acaricidal action of a formulation containing fluralaner (Exzolt®) against A. galli.Methods The study was carried out from November 2019 to January 2020. Thirty-four laying hens naturally infested with A. galli were equally divided into a fluralaner-treated group and an untreated control group. The fluralaner-treated group received Exzolt® in drinking water at a dose of 0.05 mL/kg body weight (equivalent to 0.5 mg fluralaner/kg body weight), twice, seven days apart. Both groups were followed for 70 days evaluating the level of infestation by counting mites in skin scrapings and assessment of skin lesions.Results The average mite count of the treated group decreased significantly, dropping from 61.6 to 3.8 mites (D+7 to D+70). The efficacy progressively increased on subsequent days, reaching 98.8% on day +56 post-treatment and 96.9% on day +70. Recovery of skin lesions was observed after administration of Exzolt®, showing a marked remission in the degree of lesions (2.5 on day -14 to 0.2 on day +70). The mean number of mites in the untreated control group ranged from 79.3 to 124.1 and the lesion score from 2.6 to 2.9, thus remaining stable throughout the study.Conclusion The results obtained in the present study demonstrated that Exzolt® administered at a dose of 0.05 mL of product/kg body weight (equivalent to 0.5 mg of fluralaner/kg body weight), twice at a seven-day interval, in drinking water was effective in the treatment of the mite Allopsoroptoides galli in naturally infested laying hens.
Assessment of GRACE/GRACE Follow-On Estimates of Global Mean Ocean Mass Change
Authors: Kim Jae-Seung , Seo Ki-Weon , Chen Jianli , Wilson Clark 
Published: December 2021

Abstract
Global mean sea level has increased ~3.5 mm/yr over several decades due to increases in ocean mass and changes in sea water density. Ocean mass, accounting for about two-thirds of the increase, can be directly measured by the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GFO) satellites. An independent measure is obtained by combining satellite altimetry (measuring total sea level change) and Argo float data (measuring steric changes associated with sea water density). Many previous studies have reported that the two estimates of global mean ocean mass (GMOM) change are in good agreement within stated confidence intervals. Recently, particularly since 2016, estimates by the two methods have diverged. A partial explanation appears to be a spurious variation in steric sea level data. An additional contributor may be deficiencies in Glacial Isostatic Adjustment (GIA) corrections and degree-1 spherical harmonic (SH) coefficients. We found that erroneous corrections for GIA contaminate GRACE/GFO estimates as time goes forward. Errors in GIA corrections affect degree-1 SH coefficients, and degree-1 errors may also be associated with ocean dynamics. Poor estimates of degree-1 SH coefficients are likely an important source of discrepancies in the two methods of estimating GMOM change.
Evolution of Physicochemical Properties of 2-(2-(4-(4-chloro) phenyl) vinyl)-1, 1, 3-trimethyl-1H-benzo[e] Indolium Iodide via Experimental and Quantum Chemical Calculation for Third-Harmonic Generation Applications
Authors: Palaniyasan Eniya , Radhakrishnan Anbarasan , Maidur Shivaraj R. , Patil Parutagouda Shankaragouda , Jeyaperumal Kalyana Sundar 
Published: December 2021

Abstract
In this work, the authors have reported the synthesis, crystal structure, and physicochemical properties of new Indolium based nonlinear optical crystal of 2-(2-(4-(4-chloro) phenyl) vinyl)-1, 1, 3-trimethyl-1H-benzo[e] Indolium iodide. The title crystal was grown by slow evaporation technique using methanol as a solvent. The harvested crystal is subjected to the single-crystal X-ray diffraction study and found that the title crystal belongs to the monoclinic system with a space group of P21/n. The bandgap and optical window of the material are studied by UV-visible spectroscopic analysis, and the found values are 4.2 eV and 295-800 nm, respectively. The grown crystal is subjected to analyze the thermal stability and found that high-temperature stability value upto 264 oC and which is sufficient for industrial applications. Furthermore, the effect of temperature on the grown crystal dielectric properties such as dielectric constant and dielectric losses is investigated by impedance spectroscopic analysis. The molecular charge transfer on the material is studied from density functional theory. The various intermolecular interactions existence in the crystal is explored from Hirshfeld surface analysis. Finally, the Z-scan parameters expose that title crystal is a potential material for third-harmonic generation applications. The overall structural studies and functional properties disclose that the grown crystal is well suitable for harmonic generation applications.
The Risk of Prostate Cancer Associated With Common Mutations of AR Gene in Iranian Population: a Perspective Study of Personalized Treatment
Authors: Rahbaran Marzieh , Doabsari Maryam Hassani , Sharifi Farshad , Afshari Mahdi , Hasanzad Mandana 
Published: December 2021

Abstract
Background: Prostate cancer (PC) is one of the most common cancers among men. Genetic predisposition is emerging as a risk factor for PC development. The Androgen receptor (AR) gene is associated with the development and prognosis of PC. Understanding the AR mutations is very important in the precision treatment of PC-resistant patients to androgen deprivation therapy. In this study, we investigate any association between common AR mutations with the risk of PC.Methods and results: In this case-control study, blood samples were collected from 121 radical prostatectomy (RP) patients who were pathologically diagnosed with PC and 120 benign prostate hyperplasia (BPH) subjects as a control group. The targeted area of the AR gene was amplified by PCR and confirmed by the Sanger sequencing method. The target area of the AR gene screened for 124 alterations in intron 7, 44 mutations in exon 8, and 52 variants in the 3'UTR region. rs113528927 DelIns AC>ACACACCAC had the most frequent mutant alleles between case and control groups, but this genotype distribution among the two recruited groups was not significant. Only one mutation, c.2644C>A, was observed in exon 8 in BPH subjects, and six alterations were detected in 3'UTR.Conclusions: For the first time in the Iranian population, AR common mutations were screened in PC patients, and our results indicate no relationship with the risk of PC, which means that other potential molecular risk factors may be engaged for PC in our population.
Bioinformatics analysis of UNC93B1 gene and prognostic value in breast cancer
Authors: Yi Xiaoqiong , Shi Huadi , Shi Zhenyi , Zhong Fulan , Wu Yingying , Ou Feiyan , Xu Zumin , Chen Xinggui 
Published: December 2021

Abstract
Background: The Unc-93 homolog B1 (UNC93B1) is a transmembrane protein that is associated with immune diseases such as influenza, herpes simplex encephalitis, and systemic lupus erythematosus; however, the role of UNC93B1 in cancer (including human breast cancer) is known less. The analysis of the association between UNC93B1 expression and breast cancer survival is also unclear. Methods: We used multiple online databases including Oncomine, GEPIA, bcGenExMiner v4.6 and PrognoScan, to conduct bioinformatics analysis of clinical parameters and survival data related to UNC93B1 in breast cancer patients. Results: It was found that UNC93B1 was expressed in different subtypes of breast cancer compared to normal tissues. Scarff-Bloom-Richardson (SBR) classification, Nottingham prognostic index (NPI), estrogen receptor (ER) negative, progesterone receptor (PR) negative epidermal growth factor receptor-2 (HER2) positive and lymph node positive are positively correlated with the UNC93B1 level. We found that increased expression of UNC93B1 was associated with worse relapse-free survival, disease-specific survival, and overall survival. We also confirmed the positive correlation between UNC93B1 and ALDH3B1 gene expression. Conclusion: The lower expression of UNC93B1 was correlated with better clinical prognostic parameters and clinical survival in breast cancer on the basis of the bioinformatic analysis.
WITHDRAWN: Analysis of Autophagy-Related Signatures in The Tumor Immune Microenvironment and Identification of Clinical Prognostic Markers in Bladder Carcinoma
Authors: Cao Huifeng , Chen Dayin , Zhang Zhihui , Cheng Liang , Luo Zhenguo , Yu Junjuan 
Published: December 2021

Abstract
Objectives: Bladder carcinoma (BLCA) is one of the most common malignant diseases of urinary system. Our study aimed to investigate the autophagy-related signatures in the tumor immune microenvironment and construct effective prognosis prediction model.Methods: RNA sequencing data and corresponding clinical information of BLCA were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Autophagy-related genes were extracted from TCGA dataset for consensus clustering analysis, and differences in survival rate were analyzed. STIMATE algorithm was used to analyze the tumor microenvironment (TME) and immune cell infiltration was compared between different clusters. Differentially expressed genes (DEGs) between different clusters were identified, followed by function annotation. Independent prognostic signatures were further revealed to construct prognostic prediction model.Results: We identified 35 autophagy-related genes associated with prognosis. Survival rate of samples in cluster 1 was significant lower than that in cluster 2. Cluster 2 had markedly lower tumor purity and significantly higher estimate score and stromal score than cluster 1. The proportions of T cells CD8, macrophages M1, T cells CD4 memory activated, NK cells activated, and dendritic cells activated were higher in cluster 1. There were 1,275 DEGs which were mainly enriched in functions and pathways related to immune response and cancer. Seven genes (ATF6, CAPN2, NAMPT, NPC1, P4HB, PIK3C3, and RPTOR) were further identified as the independent prognostic signatures to construct risk score prediction model, which had good prediction performance.Conclusion: Prognosis prediction model based on 7 autophagy-related genes may have great value in BLCA prognosis prediction.
Dissection of Maize Drought Tolerance at the Flowering Stage Using Ge-Nome-Wide Association Study
Authors: Khan Siffat Ullah , Zheng Yanxiao , Chachar Zaid , Zhang Xuhuan , Zhou Guyi , Zong Na , Leng Pengfei , Zhao Jun 
Published: December 2021

Abstract
Drought is one of the most critical environmental factors constraining corn production especially when it occurs during flowering, resulting in serious yield losses. In this study, anthesis to silk interval (ASI), plant height (PH), and ear biomass at the silking date (EBM) of 279 inbred lines were evaluated under water-stress (WS) and well-water (WW) field conditions for three consecutive years. Averagely, ASI was extended by 25.96%, ear biomass was decreased by 17.54%, and the PH was reduced by 12.47% under drought stress conditions. Genome wide association studies (GWAS) were carried out using phenotypic values under WS, WW and drought-tolerance index (WS-WW or WS/WW) applying mixed linear model controlling both population structure and relative kinship. Totally, 71, 159, and 21 SNPs were significantly (P < 10-5) associated with ASI, ear biomass, and PH, respectively. Candidate genes encoding ARABIDILLO 1 protein, glycoprotein, Tic22-like and Zinc finger family protein for ASI, and 26S proteasome non-ATPase regulatory subunit-9 for EBM, were identified under both WW and WS conditions. Pyridoxal phosphate transferase was associated with EBM under drought stress treatment in consecutive two years. Furthermore, most candidate genes were evidenced to be drought responsive in the association panel. Meanwhile, the favourable/drought tolerance haplotypes were identified based on haplotype analysis. These findings provide insights into the genetic basis of drought tolerance at the flowering stage especially for the female inflorescence development and will facilitate high drought tolerant maize breeding.
Effect of Eucommia ulmoides leaves on hyperuricemia and kidney injury induced by a high-fat/high-fructose diet in rats
Authors: Gong Man , Zhang Hong , Liu Xiaoqian , Li Qingxia , Zhang Yang , Zhang Weijin , Huang Na , Chen Anying , Dai Liping , Wang Zhimin 
Published: December 2021

Abstract
Eucommia ulmoides leaves have unique advantages in the treatment of metabolic diseases. In this study, network pharmacology and molecular-docking methods were used to predict the effects and action mechanisms of the major components of E. ulmoides leaves on hyperuricemia. Combining literature collection, we used SciFinder and the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform to collect E. ulmoides leaf flavonoid and iridoid components. Swiss Target Prediction, SEA, GeneCards, and the OMIM database were used to obtain core targets, and the STRING protein database was performed as core targets for gene ontology enrichment Set and KEGG analyses. Molecular docking was applied to predict the pathways regulating the metabolism of uric acid. The selected targets and targeting efficacy were validated using a rat model of hyperuricemia and renal injury induced by a high-fat and high-fructose diet. A total of 32 chemical components with effective targets, which regulated the PI3K-AKT pathway and endocrine resistance, were collected. Isoquercetin, kaempferol, and quercetin were predicted via network pharmacology to have potential bioactivities and strong docking binding forces. Molecular docking results showed that iridoids and flavonoids are bound to proteins related to inflammation and uric acid metabolism. In addition, it was verified via animal experiments that an E. ulmoides leaf extract ameliorated hyperuricemia, renal injury, and inflammation, which are closely related to the targets IL-6, TNF-α, TLR4, and GLUT9. In conclusion, E. ulmoides leaf flavonoids and iridoids ameliorate hyperuricemia and uric-acid–induced inflammation through a multi-component, multi-target, and multi-pathway mechanism, which provides a theoretical basis for the development of therapeutics from E. ulmoides leaf components.
Deep Learning Based Signal Processing For Multiuser OFDM Systems
Authors: Ye Youjie , Chen Yunfei 
Published: December 2021

Abstract
Deep learning (DL) methods have been proved effective in improving the performance of channel estimation and signal detection. In this work, we propose three DL algorithms: fully connected deep neural network (FCDNN), convolutional neural networks (CNN), and long short-term memory (LSTM) neural networks for signal processing in multiuser orthogonal frequency-division multiplexing (OFDM) communications systems. The bit error rates (BERs) of these DL methods are compared with the conventional linear minimum mean squared error (LMMSE) detector. Additionally, the relationships between the BER and signal-to-interference ratio (SIR), signal-to-noise ratio (SNR), the number of interfering users (NoI) and modulation type are investigated. Numerical results show that all DL methods outperform LMMSE under different multiuser interference conditions, and FCDNN and LSTM give the best and robust anti-multiuser performance. This work shows that FCDNN and LSTM network have strong anti-interference ability and are useful in multiuser OFDM systems.
Study on Surface Quality and Corrosion Resistance of Ultrasonic Vibration Assisted Micromilling Inconel718
Authors: Yuan Zhonghang , Fang Bin , Dong Yude , Ding Heng , Zhang Yuanbin 
Published: December 2021

Abstract
Micromilling (MM) is favored by the field of high-precision micro parts. However, the high plasticity of Inconel718 often poses a threat to MM, such as pits, humps and gullies, which affect the surface quality. In this study, the influence of ultrasonic vibration assisted micromilling (UVAMM) on surface quality is comprehensively analyzed by using the machining process of workpiece vibration, combined with cutting force, tool wear, surface morphology and corrosion resistance. The results show that, on the one hand, small amplitude plays a significant role in reducing cutting force and inhibiting tool wear. On the other hand, smaller speed, smaller feed rate and moderate amplitude will produce better surface morphology, which is a uniform and regular fish scale surface with lower surface roughness and fewer surface defects. Furthermore, the application of ultrasonic vibration also significantly improves the surface corrosion resistance of Inconel718. It is worth noting that the surface corrosion resistance does not completely depend on the surface roughness, but also has a close correlation with the surface morphology.
Theoretical Researches On Binding Modes and Stability of Hydrogen Bonds Between Uracil and Formic Acid
Authors: Tang Qian , Huang Ting , Huang Ruisi , Cao Hongyu , Wang Lihao , Zheng Xuefang 
Published: December 2021

Abstract
The hydrogen bond formation with formic acid would affect the complementary pair of bases between uracil and adenine, but the binding modes and spectral properties of hydrogen bonds are still obscure. Density functional theory and time-dependent density functional theory were applied to investigate the intermolecular hydrogen bonds between uracil and formic acid. The reduced density gradient (RDG), bond lengths and vibration absorption frequencies revealed that the most probable uracil-formic acid (U-FA) interaction mode formed in the position c of FA and the site 1 of U, that is, the mode 1c. The theoretical parameters in excited state complexes manifested that the variety of hydrogen bond configurations led to different degrees of strengthening or weakening of molecular interaction. In the implicit solvent (water), the formations of O-H∙∙∙O in the uracil-formic acid complexes were promoted obviously. These theoretical studies would positively affect the researches of life science and medicinal chemistry.