bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Mechanisms and Experimental Verification of Cuscuta-Salvia (Tusizi-Danshen) in the Treatment of Polycystic Ovary Syndrome (PCOS) via Network Pharmacology
Authors: Zhang Ying-ying , Ma Jian-xiong , Zhu Yu-tian , Wang Yi-xuan , Chen Wang-qiang , Sun Xin , Zhang Wei , Wang Chen-ye , Ding Caifei 
Published: July 2021

Abstract
Polycystic ovary syndrome (PCOS) is a sort of endocrine disease associated with Reproduction. The formula of Cuscuta-Salvia has been widely used in treatment of PCOS in clinic. However, its chemical and pharmacological are still not known in detail. First, the active components of Cuscuta-Salvia were identified by UHPLC-ESI-Q-TOF-MS and screened from TCMSP database, and the disease targets were obtained from the DisGeNET and GeneCards databases. Subsequently, common targets between Cuscuta-Salvia and PCOS were obtained via a Venn diagram. Second, a protein-protein interaction (PPI) network was established. Core genes were selected using Cytoscape software plugin. Third, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed for common targets using “pathview” package in R. Finally, several core targets were verified via determination of qRT-PCR and HE staining. Combined UHPLC-ESI-Q-TOF-MS analysis with network pharmacology study, 14 active components were obtained. Eighty common targets were also obtained. Ten core genes were regulated by Cuscuta-Salvia in PCOS, including IL6, AKT1, VEGFA, TP53, TNF, MAPK1, JUN, EGF, CASP3, and EGFR. GO results showed that cellular response to drug, response to oxygen levels, response lipopolysaccharide, and response to molecule of bacterial origin in biological process (BP) category; membrane, transcription regulator complex, nuclear chromatin, postsynaptic membrane, and vesicle lumen in cellular component (CC) category; DNA-binding transcription factor binding, RNA polymerase II-specific DNA-binding transcription factor binding, DNA-binding transcription activator activity, RNA polymerase II-specific, DNA-binding transcription activator activity, and cytokine receptor binding in molecular function (MF) terms. KEGG enrichment pathway mainly involves in PI3K−Akt signaling pathway, MAPK signaling pathway, cellular senescence, TNF signaling pathway, and IL-17signaling pathway. Furthermore, based on an experimental study, Cuscuta-Salvia ameliorated the pathology of ovary, live and adipose tissue. Additional, Cuscuta-Salvia increased the mRNA expression of VEGFA. Cuscuta-Salvia decreased the mRNA expression of IL6, AKT1, TP53, MAPK1, JUN, EGF, AR, LHb, CYP17a1, and CYP19a1. Our results demonstrate that Cuscuta-Salvia may provide a novel pharmacology basis in an experimental model of PCOS via the regulation of the gene expression. This study lays a basis for subsequent research and clinical application.
Interleukin-10 Improves Stroke Outcome by Controlling the Detrimental Interleukin-17A Response
Authors: Piepke Marius , Clausen Bettina H. , Ludewig Peter , Vienhues Jonas H. , Bedke Tanja , Javidi Ehsan , Rissiek Björn , Jank Larissa , Brockmann Leonie , Sandrock Inga , Degenhardt Karoline , Prinz Immo , Flavell Richard A. , Kobayashi Yasushi , Renne Thomas , Gerloff Christian , Huber Samuel , Magnus Tim , Gelderblom Mathias 
Published: July 2021

Abstract
Background: Lymphocytes have dichotomous functions in ischemic stroke. Regulatory T cells are protective, while IL-17A from innate lymphocytes promotes the infarcts growth. With recent advances of T cell-subtype specific transgenic mouse models it now has become possible to study the complex interplay of T cell subpopulations in ischemic stroke.Methods: In a murine model of experimental stroke we analyzed the effects of IL-10 on the functional outcome for up to 14 days post-ischemia and defined the source of IL-10 in ischemic brains based on immunohistochemistry, flow cytometry, and bone marrow chimeric mice. We used neutralizing IL-17A antibodies, intrathecal IL-10 injections, and transgenic mouse models which harbor a deletion of the IL-10R on distinct T cell subpopulations to further explore the interplay between IL-10 and IL-17A pathways in the ischemic brain. Results: We demonstrate that IL-10 deficient mice exhibit significantly increased infarct sizes on days three and seven and enlarged brain atrophy and impaired neurological outcome on day fourteen following tMCAO. In ischemic brains IL-10 producing immune cells included regulatory T cells, macrophages, and microglia. Neutralization of IL-17A following stroke reversed the worse outcome in IL-10 deficient mice and intracerebral treatment with recombinant IL-10 revealed that IL-10 controlled IL-17A positive lymphocytes in ischemic brains. Importantly, IL-10 acted differentially on αβ and γδ T cells. IL-17A producing CD4+ αβ T cells were directly controlled via their IL-10-receptor (IL-10R), whereas IL-10 by itself had no direct effect on the IL-17A production in γδ T cells. The control of the IL-17A production in γδ T cells depended on an intact IL10R signaling in regulatory T cells (Tregs). Conclusions: Taken together, our data indicate a key function of IL-10 in restricting the detrimental IL-17A-signaling in stroke and further supports that IL-17A is a therapeutic opportunity for stroke treatment.
Worse Cardiovascular and Renal Outcome in Male SLE Patients: First Gender Study From the Swiss SLE Cohort Study
Authors: Mihailovic Jelena , Ribi Camillo , Chizzolini Carlo , Trendelenburg Marten , Von Kempis Johannes , Huynh-Do Uyen , Dahdal Suzan 
Published: July 2021

Abstract
Background: Systemic lupus erythematosus (SLE) in males is rare and poorly understood. Thus, still little is known about gender differences in SLE. We set out to identify gender differences regarding clinical manifestations as well as renal and cardiovascular outcomes of SLE. Methods: We analyzed patient data from the Swiss SLE Cohort Study. Cumulative clinical manifestations according to the updated American College of Rheumatology criteria were recorded at inclusion. Cardiovascular events were recorded within Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index (SLICC-SDI). Renal failure was defined as eGFR<15 ml/min/1.73m2, initiation of renal replacement therapy or doubling of serum creatinine which were all assessed yearly or documented as end stage renal disease in SLICC-SDI. Risk differences were calculated using logistic regression and cox regression models.Results: We analyzed 93 men and 529 women with a median follow up time of 2 years. Males were significantly older at diagnosis (44.4 versus 33.1 years, p<0.001) and had less often arthritis (57% versus 74%, p=0.001) and dermatological disorders (61% versus 76%, p<0.01). In multivariate analysis female gender remained a significantly associated with arthritis and dermatological disorders. After adjusting for age, disease duration, ethnicity, time to diagnosis, medication and eGFR and SELENA SLEDAI at inclusion men had a significantly higher hazard ratio of 2.5 for renal failure (95% confidence interval (95%-CI) 1.1-6.0, p<0.04). Total SLICC-SDI Score was comparable. Men had significantly more coronary artery disease (CAD) (17% versus 4%, p<0.001) and myocardial infarction (10% versus 2%, p<0.01). In multivariate analysis, male gender remained a significant risk factor for CAD (odds ratio (OR) 5.6, 95%-CI 2.3-13.7, p<0.001) and myocardial infarction (OR 8.3, 95%-CI 2.1-32.6, p=0.002).Conclusion: This first gender study in a western European population demonstrates significant gender differences in SLE. Male gender is an independent risk factor for cardiovascular events and renal failure in SLE. Potential etiological pathomechanisms such as hormonal or X-chromosomal factors remain to be further investigated.
How Marine Tourism Promote Financial Development in Sustainable Economy: New Evidence from South Asia
Authors: Li Liu , Wu Baijun , Patwary Ataul Karim 
Published: July 2021

Abstract
The ocean economy and marine tourism policies are global economic concerns being looked at from a deeply holistic viewpoint. For South Asian countries, the ocean economy and marine tourism have successive socio-economic importance. The quantification of the ocean economy and marine tourism also poses some major challenges and these challenges pose limitations for policymaking by the government and other relevant agencies. The study has used the newly developed Hidden Panel Cointegration test and Nonlinear Panel Autoregressive Distributed Lag (NPARDL) model for a relationship between economic growth and tourism is assessed. This study offers consistent and reliable results of co-integration by incorporating the findings of four approaches to co-integration. The empirical results illustrate the asymmetric relationship between ocean and marine tourism and economic growth. The findings showed that 1% increase in long term tourism economic growth is adjusted by 2.95% annually. This research paper aims to provide a policy related to South Asia's economic activities and ocean and marine tourism economic significance. Protecting local marine protected areas (MPAs) will improve the economic benefits of the ocean and the marine economy. The policy suggests that there should be a law ensuring that marine tourism is of high quality and environment friendly. This paper provides a guideline for further research with a strong emphasis on ocean-and marine-related economic development and tourism.
Vidarabine, An Anti-Herpes Agent, Prevents Occlusal-Disharmony-Induced Cardiac Dysfunction in Mice
Authors: Hayakawa Yoshio , Ohnuki Yoshiki , Suita Kenji , Mototani Yasumasa , Ishikawa Misao , Ito Aiko , Morii Akinaka , Kiyomoto Kenichi , Tsunoda Michinori , Matsuo Ichiro , Kawahara Hiroshi , Okumura Satoshi , Nariyama Megumi 
Published: July 2021

Abstract
We recently reported a positive relationship between occlusal disharmony and cardiovascular disease via activation of β-adrenergic signaling in mice. Furthermore, inhibition of type 5 adenylyl cyclase (AC5), a major cardiac subtype in adults, protects the heart against oxidative stress. Here, we examined the role of AC5 in the development of occlusal-disharmony-induced cardiovascular disease in bite-opening (BO) mice, prepared by cementing a suitable appliance onto the mandibular incisor. We first examined the effects of BO treatment on cardiac function in mice treated or not treated for 2 weeks with vidarabine, which we previously identified as an inhibitor of cardiac AC. Cardiac function was significantly decreased in the BO group compared to the control group, but vidarabine ameliorated the dysfunction. Cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage were significantly increased in the BO group, but vidarabine blocked these changes. The BO-induced cardiac dysfunction was associated with increased phospholamban phosphorylation at threonine-17 and serine-16, as well as increased activation of the Ca2+-calmodulin-dependent protein kinase II/receptor-interacting protein 3 signaling pathway. These data suggest that AC5 inhibition with vidarabine might be a new therapeutic approach for the treatment of cardiovascular disease associated with occlusal disharmony.
Factors Affecting the Overweight and Obesity of Students Aged 11 to 17 in Low and Middle-Income Countries Based on GSHS Questionnaire Data
Authors: Shadmehr Rafat , Moradinazar Mehdi , Shakiba Ebrahem , Najafi Farid , Mostafaee Shayan , Rezaeian Shahab , Harsini Pooyan Afzali , Safari Roya , Ramazani Uosef , Moradi Zeinab 
Published: July 2021

Abstract
Background:Obesity and overweight in early life arebeingone of the challenges of public health in both developed and developing societies.Methods:This cross-sectional study was designed on the Global School-based Student Health Survey (GSHS) data from the WHO, in collaboration with UNAIDS, UNESCO, and UNICEF. The countries of low- and middle-income in the six WHO regions were included.BMIis classified into three classes: overweight, normal-weight, and underweight based on length/height, weight, and age.Results: Of the 187893 students aged 11 to 17 years, 43220 (23.0%) were overweight. The prevalence of obesitywas higher in boys (23.67% and 22.39% in boys and girls respectively).The probability of obesity decreases by the age of the students. There was a positive relationship between following personal hygieneprinciples and overweight so that the use of soap and washing hands after going to the toilet increased the odds of overweight by 17% and 11%, respectively.Each unit increased the prevalence of overweight in adulthood (1.07 - 1.04 OR = 1.06), and each unit increase in Gini Index (OR = 1.03,1.00-1.05) on average increased the odds of overweight in students aged 11 to 17 years, but for the prevalence of overweight in children less than 5 years old, GDP and SDGs did not have any significant effect onoverweight in children aged 11 to 17 years.Conclusion:The country-level Gini index and the prevalence of overweight and obesity in adults had a significant role in overweight and obesityin students. Due to the different situation of the countries, it is required to plan specific programs to tackle overweight in children.
Eosinopenia Predicting Long-Term Mortality In Hospitalized Acute Exacerbation of COPD Patients With Community-Acquired Pneumonia – A Retrospective Analysis
Authors: Mao Yanxiong , Qian Yuanyuan , Sun Xiaoyan , Li Na , Huang Huaqiong 
Published: July 2021

Abstract
Background: Chronic obstructive pulmonary disease (COPD) and community-acquired pneumonia (CAP) are both common respiratory diseases. Acute exacerbation of COPD (AECOPD) could be triggered by CAP. Peripheral blood eosinopenia are strongly associated with increased mortality. In hospitalized AECOPD patients with CAP, eosinopenia may be used to identified patients with high risk of death on admission.Methods: We conducted a retrospective cohort study of hospitalized AECOPD patients with CAP. Patients who had received systemic corticosteroids preadmission were excluded. The patients were identified by individual case file review. According to blood eosinophil count, patients were grouped as eosinopenia(<50/μL) and non-eosinopenia (≥50/μL). Associations of eosinopenia with infection and 18-month survival were analyzed using appropriate statistical methods.Results: Baseline demographic, comorbidity and CURB65 score were similar between two groups. The eosinopenia group had significantly higher pro-BNP, D-dimer, neutrophil percentage, and lower lymphocyte count and lymphocyte percentage. The eosinopenia group had significantly higher CRP (median 77.30 vs 16.55, p=0.008) and procalcitonin (median 0.32 vs 0.12, p=0.001). Survival at 18 months after hospital discharge was significantly lower in the eosinopenia group vs non-eosinopenia group (log rank, p=0.002)Conclusions: Eosinopenia (<50/μL) was a strong predictor of 18-month mortality, and associated with more severe infection in hospitalized AECOPD patients with CAP. Eosinophil count at admission can be used as a prognosis marker of mortality in those population.
Review on Factors Affecting Diagnostic Yield in Stereotactic Biopsy for Brain Lesions: A 5-Year Single-Center Series.
Authors: Lau Bik Liang , Vijian Kugan , Liew Donald Ngian San , Wong Albert Sii Hieng 
Published: July 2021

Abstract
Objective: To determine the factors that are associated with the diagnostic yield of stereotactic brain biopsy. Materials and Methods: A retrospective analysis was performed on 50 consecutive patients who underwent stereotactic brain biopsies in a single institute from 2014 to 2019. Variables including age, gender, lesion topography and characteristics, biopsy methods and surgeon’s experience were analyzed along with diagnostic rate. Results: This study included 31 male and 19 female patients with a mean age of 48.4 (range: 1-76). Of these, 25 underwent frameless brain-suite stereotactic biopsies, 15 were frameless portable Brain-lab® stereotactic biopsies and 10 were frame-based CRW® stereotactic biopsies. There was no statistical difference between the diagnostic yield of the three methods. The diagnostic yield in our series was 76%. Age, gender and biopsy methods had no impact on diagnostic yield. Periventricular and pineal lesion biopsies were significantly associated with negative diagnostic yield (p=0.01) whereas larger lesions were significantly associated with a positive yield (p=0.01) with the mean volume of lesions in the positive yield group (13.6cc) being higher than the negative yield group (7cc). The diagnostic yields seen between senior and junior neurosurgeons in the biopsy procedure were 95% and 63% respectively (p=0.02). Conclusion: Anatomical location of the lesion, volume of the lesion and experience of the surgeon have significant impacts on the diagnostic yield in stereotactic brain biopsy. There was no statistical difference between the diagnostic yield of the three methods, age, gender and depth of lesion.
Theoretical Investigation on the Complexation Characteristics and Spectral Properties of CdTe QDs With Four Capping Agents
Authors: Yang Qinghong , Wan Xujiang , Chen Yang , Luo Hui , Zheng Yan , Li Laicai 
Published: July 2021

Abstract
In this paper, density functional theory (DFT) and time-dependent density functional theory (TDDFT) are used to study the complexation characteristics CdTe quantum dots with four different capping agents, i.e. : 3-mercaptopropionic acid (MPA), reduced glutathione (GSH), 1-thioglycerol (TG) and 2-mercaptoethanesulfonate (MES). The properties of these complexes are analyzed by the complexation energy, bond lengths, electron densities, Mulliken charges and frontier molecular orbitals. In addition, the UV-Vis absorption spectra of pure CdTe QDs and those stable complexes are calculated. The results indicate that the four capping agents could form stable complexes with CdTe QDs. However, there are also some differences. For instance, the complexation between MES and QDs is the most stable and the electron amount transferred from MES to CdTe QDs is the most while the absorption intensity of UV-visible light after complexation is the largest. The stability of the complexes are followed by MPA and TG and the complexation between GSH and QDs is the most unstable, which is accompanied with the minimal electron transfer amount and the weakest absorption intensity of UV-visible light. The maximum absorption wavelengths of CdTe QDs are consistent with the experimental observed wavelength, which explains the experimental phenomena excellently.
Monitoring the indirect impact of Covid-19 on hospital care pathways in Italy: results from the Mimico-19 network
Authors: Spadea Teresa , Girolamo Chiara Di , Landriscina Tania , Leoni Olivia , Forni Silvia , Colais Paola , Fanizza Caterina , Allotta Alessandra , Onorati Roberta , Gnavi Roberto 
Published: July 2021

Abstract
The COVID-19 pandemic has a non-negligible indirect impact on hospital care pathways, which is important to estimate. To this aim, we set up the Mimico-19 network of seven Italian regions (62% of the Italian population) representing different socio-demographic areas of the country with also a different burden of the epidemic. We retrospectively analysed regional hospital discharges data, computing twelve indicators of volumes and performance in three clinical areas: cardiology, oncology and orthopaedics, including time-dependent pathways and elective surgery. Weekly indicators for the period January-July 2020 were compared with the average of the corresponding indicators in 2018 and 2019; comparisons were performed within 3 sub-periods: pre-lockdown, lockdown and post-lockdown.
The weekly trend of hospitalizations for ST-segment elevation myocardial infarction (STEMI) showed a 40% reduction, but the proportion of STEMI patients with a primary PTCA did not significantly change from previous years. Volumes of malignant neoplasms surgeries differed substantially by site, with a limited reduction for lung cancer (<20%) and greater declines (30-40%) for breast and prostate cancers. Hospitalizations for femoral neck fracture in the elderly decreased by 20%, but the percentage of timely interventions remained constantly higher than the previous years. General trends did not show important differences across regions, regardless of the different Covid-19 burden.
Hospitalizations have generally decreased, but the capacity of a timely and effective response in time-dependent pathways of care was not jeopardized throughout the period. The drop in the care demand for cardiovascular diseases and cancers needs to be further investigated and monitored more thoroughly.
Clinical Guidelines for Managing Hearing Loss as a Complication of Drug-Resistant Tuberculosis Treatment: An Evaluation of Implementation fidelity in Kano, Nigeria
Authors: Muhammad Sani Ibrahim , Eboreime Ejemai Amaize , Ogbonna Vivian Ifeoma , Zubairu Iliyasu , Ibisomi Latifat 
Published: July 2021

Abstract
Background: Nigeria has a high burden of Tuberculosis (TB) including Drug-resistant Tuberculosis (DR-TB) and hearing lossObjective: To measure the implementation fidelity of management guidelines for hearing loss resulting from DR-TB treatment and to identify its determinants Method: A questionnaire-based cross-sectional study was conducted at the Infectious Disease Hospital, Kano. Implementation fidelity of the Programmatic Management guidelines for the treatment of Drug-resistant Tuberculosis was measured under the four domains of content, coverage, duration and frequency. The determinants examined are intervention complexity, facilitation strategies, quality of delivery and participant responsiveness as proposed by the Carroll et al framework. Other determinants used are age, sex, professional cadre and work experience of healthcare providers.Results: The Implementation fidelity score ranged from 40 - 64% with a mean of 47.6%. Quality of delivery, intervention complexity, participants’ responsiveness, and being a medical doctor exerted a positive effect on implementation fidelity while facilitation strategy, age and work experience exerted a negative effect on implementation fidelity. Conclusion: The implementation fidelity of management guidelines for hearing loss resulting from DR-TB treatment was low. Implementation fidelity should be assessed early and at intervals in the course of implementing the PMDT guideline and indeed, in the implementation of any intervention.