bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxicity, Apoptosis induction and change of p53, PARP, p21 and Bcl-2 genes expression in the human anaplastic thyroid carcinoma cells line (SW-1736) with curcumin
Authors: Varnamkhasti Khalil Khashei , Tavakoli Parinaz Tavakoli , Rouhi Leila , Raisi Somayee 
Published: June 2021

Anaplastic thyroid carcinoma is highly invasive with a poor response to a treatment. In this study, curcumin bioavailability and its effects on apoptosis induction and selected genes expression of the human anaplastic thyroid carcinoma cell line (SW-1736) were examined. SW-1736 cells were incubated for 24 and 48 hours with different concentrations of curcumin 2.5, 5, 7.5 and 10 μM to examine bioavailability, and for 24, 48 and 72 hours with concentrations of 2.5, 5, 7.5, 10 μM and 2.5, 5 and 10 μM respectively to examine apoptosis and the expression of p53, PARP, p21 and Bcl-2 genes. Then, bioavailability was analyzed by MTS kit, apoptosis was analyzed by flow- cytometry using Annexin V-FITC/PI kit and the expression of p53, PARP, p21 and Bcl-2 genes were analyzed by Real Time PCR. ANOVA test and SPSS 16 software were used for statistical analysis. The results indicate that curcumin at the concentration of 7.5 μM has significantly decreased bioavailability in anaplastic thyroid cells in comparison with other treatments at both incubation periods. Induction of apoptosis with increasing concentration of curcumin in dose and time dependent manner increased in this cell line. Also, treatment with curcumin significantly decreased the expression of Bcl-2 gene and increased the expression of p53, PARP and p21 genes in some experimental groups compared to the control group. Curcumin inhibited the growth, proliferation and invasion of anaplastic thyroid cancer cells through altering the expression of the genes involved in the apoptosis process.
PREVALÊNCIA DE DESVIOS POSTURAIS, NÍVEL DE FLEXIBILIDADE E DE FORÇA/RESISTÊNCIA MUSCULAR EM ADOLESCENTES ESCOLARES DE XANXERÊ/SC
Authors: Pedrozo Sandro Claro , Sartori Kadija , Bordignon Tiago , Bim Mateus Augusto 
Published: June 2021

O objetivo do estudo foi verificar a prevalência de desvios posturais nos adolescentes escolares de Xanxerê-SC e sua associação com os níveis de força/resistência abdominal e flexibilidade. A amostra foi composta por 71 escolares de 12 a 15 anos de idade, sendo 37 do sexo masculino e 34 do sexo feminino. A avaliação postural foi realizada por meio de análise de registro fotográfico nos planos frontal (anterior e posterior) e sagital (direita e esquerda), utilizando o simetrógrafo digital (Physical test 8.0). A força-resistência abdominal foi determinada teste de abdominais em 1 minuto e a flexibilidade pelo teste de sentar e alcançar. Para análise dos dados foi utilizada a estatística descritiva, o teste t de student para amostra independentes e o teste qui-quadrado. Em relação à prevalência dos desvios posturais, 38% dos escolares apresentaram algum tipo de desvio postural, sendo maior no sexo masculino (43,2%) em relação ao sexo feminino (32,4%). Os desvios mais frequentes entre os participantes do estudo foram a hiperlordose (18,3%) e a escoliose (16,7%). No sexo masculino, a escoliose (21,6%) foi mais frequente, enquanto que nas meninas, o desvio postural mais frequente foi a hiperlordose (17,6%). Também foi observada a associação (p<0,05) da zona de risco da força-resistência abdominal com a presença desvios posturais, no sexo masculino. Desta forma, conclui-se que a má postura dos adolescentes escolares da rede pública de ensino do município de Xanxerê, que participaram do estudo, está associada à baixa força-resistência muscular, podendo comprometer progressivamente a saúde da coluna vertebral.
Metode Stabilisasi Semen Terhadap Peningkatan Nilai CBR Tanah Dasar Jalan Lingkungan
Authors: Firdaus Muhammad , Suhaimi Muhammad , Fathurrozie Fathurrozie 
Published: June 2021

Dalam makalah ini mendiskusikan mengenai pengembangan fasilitas infrastruktur umum di Komplek Bumi Mas Lestari Perdagangan Banjarmasin yaitu Pembangunan Jalan lingkungan yang secara khusus meninjau bagian tanah dasar dan ketebalan timbunan sirtu. Perhitungan daya dukung tanah dasar mengacu kepada input nilai CBR metode SNI yaitu index dan engineering properties, uji kepadatan tanah standar proctor di laboratorium dan uji CBR Laboratorium. Studi penelitian terhadap tanah dasar yang dibandingkan adalah tanpa dan dengan penggunaan penambahan semen 9% terhadap tanah asli lanau lunak berlempung plastisitas rendah, hasil yang dapat mewakili untuk perbandingan nilai kelayakan subgrade adalah pemenuhan terhadap ketentuan nilai CBR subgrade pada Spesifikasi Umum Bina Marga sebesar minimum 6% pada pelaksanaan timbunan jalan, karena antara nilai CBR tanah dasar asli yang dihasilkan 4% dan setelah distabilisasi menggunakan semen menghasilkan peningkatan 9,8%, tanah dasar telah memenuhi persyaratan dan dapat digunakan menjadi bagian dari konstruksi perkerasan jalan diatasnya sebagai penopang.
Abstract
This paper discuss infrastucture development of road transportation in Bumi Mas Lestari Housing Compex Construction mainly Road access facility construction mainly for subgrade and embankment of thickness materials. The calculation of theoretical bearing capacity of subgrade based on Standar Nasional Indonesia (SNI), including Proctor Standard and CBR test laboratory.research focus on cement mixing between natural condition of subgrade using 9% cement ratio in silty clayey low plasticity type of soil sample. Conduct Bina Marga Specification of Road Construction, minimum CBR Design is 6% was allowed with 9,8% CBR Design after mixing cement as stabilitation of 4% CBR design before.
Pengaruh Penambahan Plastik LDPE Terhadap Hasil Marshall untuk HRS-WC
Authors: Hawinuti Riska , Surat Surat , Gazalie Rifanie 
Published: June 2021

Plastik sebagai hasil sampingan dari aktivitas masyarakat, menjadi penyumbang terbesar sampah di Indonesia, sehingga diperlukan penanganan khusus agar permasalahan plastik tersebut dapat terselesaikan. Salah satunya adalah dengan memanfaatkan plastik tersebut sebagai bahan tambahan campuran beraspal HRS-WC sehingga diharapkan selain berkurangnya jumlah sampah, jumlah penggunaan aspal minyak pun dapat ditekan. Tujuan penelitian ini adalah mengetahui pengaruh penambahan plastik LDPE (Low Density Poly Ethylene) terhadap karakteristik campuran beraspal HRS-WC, mengetahui perbandingan nilai karakteristik marshall antara campuran HRS-WC dengan dan tanpa tambahan plastik LDPE, menentukan kadar aspal optimum dan kadar campuran plastik LDPE optimum pada campuran HRS-WC.
Pengujian yang dilakukan di Laboratorium Struktur dan Bahan Poliban, antara lain pengujian agregat, pengujian aspal dan pengujian marshall. Benda uji yang dibuat berupa benda uji tanpa tambahan plastik dan dengan tambahan plastik sebanyak masing-masing 15 buah.
Dari hasil pengujian, campuran aspal tanpa Plastik LDPE diperoleh nilai stabilitas 1.490 kg, flow 4,20 mm, VMA 110% dan Marshall Quotient 380 kg/ mm sedangkan pada campuran dengan plastik LDPE, nilai stabilitas sebesar 1.900 kg, flow 4,20 mm, nilai VMA105% dan nilai Marshall Quotient 610 kg /mm. Dengan nilai KAO sebesar 6,50% dan kadar plastik sebesar 5,0%.
Analisis Ketersediaan Dan Kebutuhan Air Irigasi Pada Petak Sawah Di Daerah Irigasi Rawa Kecamatan Mandastana
Authors: Iriansyah Andri , Hayati Fitriani , Fakhrurrazi Fakhrurrazi 
Published: June 2021

Kabupaten Barito Kuala adalah salah satu pemerintah kabupaten yang berbatasan dengan provinsi Kalimantan Tengah dan Kalimantan Selatan, Indonesia. Dalam musim hujan pada waktu pasang air Sungai Kapuas, Sungai Barito dan air kiriman dari Kabupaten Banjar yang berbatasan langsung dengan Kecamatan Mandastana dapat membanjiri sebagian besar wilayah ini dan mengakibatkan permukaan tanah tergenang terus menerus. Masalah yang dialami oleh persawahaan di Kecamatan Mandastana adalah masalah yang mempengaruhi tata kelola air terkait saluran irigasi yang ada di pertanian itu sendiri mengalami pasang surut air, yang bergerak naik turun yang disebabkan oleh pasang air pada Sungai Barito dan Sungai Kapuas, dimana pada saat pasang area persawahan mengalami kelebihan air. Metode perhitungan yang digunakan dalam penelitian ini adalah metode mock untuk perhitungan debit andalan dan neraca air dan metode penman dan standar KP-01 untuk perhitungan kebutuhan air. Berdasarkan analisis neraca air (ketersediaan air dari curah hujan dikurang kebutuhan air disawah kondisi eksisting) didapat hasil 3,017 m3/detik, sehingga pada petak sawah terjadi kelebihan air, jadi apabila ingin dilakukan perluasan lahan persawahan ketersediaan air masih mencukupi untuk mengairi air di sawah.
Abstract
Barito Kuala Regency is one of the district governments that borders the provinces of Central Kalimantan and South Kalimantan, Indonesia. During the rainy season at the time of the tide of the Kapuas River, the Barito River and consignment water from Banjar Regency, which is directly adjacent to Mandastana District, can overwhelm most of this area and result in continuous inundation of the land surface. The problem experienced by the field in Mandastana Subdistrict is a problem that affects water management related to irrigation channels in agriculture itself which experiences tidal water, which moves up and down caused by tides on the Barito River and Kapuas River, where at the time of tide the area rice fields experience excess water. The calculation method used in this study is the mock method for calculating the mainstay discharge and water balance and penman method and the KP-01 standard for calculating water requirements. Based on the analysis of water balance (availability of water from rainfall minus water requirements under existing conditions) obtained results of 3.017 m3 / sec, so that the paddy fields occur excess water, so if you want to expand rice fields the availability of water is still sufficient to irrigate water in the fields.
Development of Psychosocial Telenursing Education Stigma of Suspected Covid-19 (Rapid Reactive) Patients
Authors: Tri Wahyuni  , Parliani  , Kharisma Pratama  , Tutur Kardiatun  , Nita Zulfia  , Paudilah  , Gmeliana Adha Sari  
Published: June 2021

In the midst of the Covid-19 pandemic, there is one social phenomenon that has the potential to aggravate the situation, namely stigma in patients suspected of Covid-19 or rapid reactive results. The lab results have not actually confirmed that the patient is positive for Covid-19 but the social environment or negative associations of a person or group of people who experience symptoms or bear certain diseases result in stigma against a person. This study aims to find out how the experience and stigma in patients suspected of Covid - 19. This study uses qualitative approach method of phenomenological studies and in-depth interviews with telenursing. The number of participants in this study was 10 participants. This study obtained 5 themes, namely physiological experience, psychological experience, biological experience, sociological experience and spiritual experience. The high positive number of Covid-19 in Indonesia makes the emergence of negative stigma of society to patients exposed to Covid-19, not even just Patients who are positive of Covid-19 who get negative stigma, therefore for diagnostic enforcement it takes examination by Molecular Test: Real Time PCR (Polymerase Chain Reaction) method. The sample types used are bronchoalveolar lavage, nasopharyngeal or oropharyngeal swabs, sputum, tracheal aspirate, nasopharyngeal aspirate or nasal wash, and biopsy tissue including from the lungs. Real Time PCR check method is the golden standard for the enforcement of diagnostic Covid - 19.
Peritonitis meconial pseudoquística por atresia intestinal perforada. Reporte de un caso
Authors: Cabrera Valerio Carlos Manuel , González Niurka , Moreno Reyes Andreina , García Risk Ariadna , Madera Noelia 
Published: June 2021

La peritonitis meconial secundaria a perforación intestinal es una complicación temida de la atresia intestinal, puesto que incrementa la morbimortalidad perinatal. Aunque sigue siendo una presentación rara, su diagnóstico debe de hacerse temprano para evitar complicaciones. Presentamos un caso clínico de un recién nacido de 38 semanas sin antecedentes prenatales indicativos de obstrucción intestinal. Quien presenta vómitos biliosos/ fecaloideos, no meconización a las 12 horas de vida extrauterina, por lo cual se le realiza radiografía de abdomen de pie y acostada que muestra marcada distensión de asas intestinales, ausencia de aire distal, edema interasas. Se realiza laparotomía exploratoria y se hace yeyunostomía para luego re-intervenirla (second look), encontrando mejoría de asas intestinales, logrando una anastomosis yeyuno-ileal término-terminal. La operación temprana se puede considerar un factor de importancia para mejorar el resultado de los pacientes con peritonitis por meconio, de igual forma se necesita de un diagnóstico temprano.
Case Report: Prenatal Diagnosis and Treatment of Fetal Autoimmune-Associated First-Degree Atrioventricular Block: First Report From China
Authors: Tang Changqing , Yu Haiyan , Shao Shuran , Hua Yimin , Chen Maoli , Zhu Qi , Li Yifei , Zhou Kaiyu , Wang Chuan 
Published: June 2021

Background: The rapid progression from fetal first-degree atrioventricular block (AVB) to third-degree AVB had been reported. However, how to define fetal first-degree AVB with proper technique and the necessity of the treatment in utero for fetal autoimmune-associated first-degree AVB are still controversial.Purpose: To explore the diagnosis and the effect of treatment for fetal first-degree AVB.Cases Presentation: Four pregnant women with positive autoantibodies anti-SSA/Ro were admitted into our hospital with complaints of rapid prolonged atrioventricular (AV) intervals of their fetuses. Fetal AV intervals were re-measured by tissue Doppler imaging (TDI) from the onset of atrial contraction to ventricular systole (Aa-Sa), which were 170 ms (case 1-twin A), 160 ms (case 1-twin B), 163 ms (case 2) and 172 ms (case 3) and 170 ms (case 4), respectively. The histories of medication usage or infection during gestation were denied. Amniotic fluid genetic screenings and virological tests were negative in all cases. No structural cardiac disorders were found and the cardiovascular profile scores were 10 for each fetus. Oral dexamethasone (initial dose of 4.5 mg daily) and hydroxychloroquine (200 mg bid) plus weekly follow-up surveillance were suggested. The dosage of dexamethasone was adjusted according to the changes of the AV intervals and fetal development of biparietal diameters (BPD) and femur lengths (FL). All fetal AV intervals were controlled well. Maternal and fetal adverse effects were noted as diabetes in 1 mother and growth retardation in all fetuses. All fetuses were delivered via cesarean section at 35+4, 37, 38, and 37+1 gestational weeks, with 10 scores of Apgar score. Postnatally, positive anti-SSA/Ro was found in all neonates. However, there were no clinical or laboratory evidence of neonatal lupus syndrome. No abnormal signs were found on postnatal electrocardiogram and echocardiography for all neonates. With a follow-up of 8–53 months, there was no progression of disease and all infants demonstrated normal physical, mental, and motor development.Conclusion: Prenatal treatment for fetal autoimmune-associated first-degree AVB could be an alternative. Strict surveillance and timely adjustment of the treatment according to the conditions of the mother and the fetus are indicated. Further studies are necessary to prove our concept.
Parametric Estimation of Reference Signal Intensity for Semi-Quantification of Tau Deposition: A Flortaucipir and [18F]-APN-1607 Study
Authors: Zhang Huiwei , Wang Min , Lu Jiaying , Bao Weiqi , Li Ling , Jiang Jiehui , Zuo Chuantao ,   and Alzheimer’s Disease Neuroimaging Initiative
Published: June 2021

BackgroundTau positron emission tomography (PET) imaging can reveal the pathophysiology and neurodegeneration that occurs in Alzheimer’s disease (AD) in vivo. The standardized uptake value ratio (SUVR) is widely used for semi-quantification of tau deposition but is susceptible to disturbance from the reference region and the partial volume effect (PVE). To overcome this problem, we applied the parametric estimation of reference signal intensity (PERSI) method—which was previously evaluated for flortaucipir imaging—to two tau tracers, flortaucipir and [18F]-APN-1607.MethodsTwo cohorts underwent tau PET scanning. Flortaucipir PET imaging data for cohort I (65 healthy controls [HCs], 60 patients with mild cognitive impairment [MCI], and 12 AD patients) were from the AD Neuroimaging Initiative database. [18F]-APN-1607 ([18F]-PM-PBB3) PET imaging data were for Cohort II, which included 21 patients with a clinical diagnosis of amyloid PET-positive AD and 15 HCs recruited at Huashan Hospital. We used white matter (WM) postprocessed by PERSI (PERSI-WM) as the reference region and compared this with the traditional semi-quantification method that uses the whole cerebellum as the reference. SUVRs were calculated for regions of interest including the frontal, parietal, temporal, and occipital lobes; anterior and posterior cingulate; precuneus; and Braak I/II (entorhinal cortex and hippocampus). Receiver operating characteristic (ROC) curve analysis and effect sizes were used to compare the two methods in terms of ability to discriminate between different clinical groups.ResultsIn both cohorts, regional SUVR determined using the PERSI-WM method was superior to using the cerebellum as reference region for measuring tau retention in AD patients (e.g., SUVR of the temporal lobe: flortaucipir, 1.08 ± 0.17 and [18F]-APN-1607, 1.57 ± 0.34); and estimates of the effect size and areas under the ROC curve (AUC) indicated that it also increased between-group differences (e.g., AUC of the temporal lobe for HC vs AD: flortaucipir, 0.893 and [18F]-APN-1607: 0.949).ConclusionThe PERSI-WM method significantly improves diagnostic discrimination compared to conventional approach of using the cerebellum as a reference region and can mitigate the PVE; it can thus enhance the efficacy of semi-quantification of multiple tau tracers in PET scanning, making it suitable for large-scale clinical application.
Combination Analysis of a Radiomics-Based Predictive Model With Clinical Indicators for the Preoperative Assessment of Histological Grade in Endometrial Carcinoma
Authors: Zheng Tao , Yang Linsha , Du Juan , Dong Yanchao , Wu Shuo , Shi Qinglei , Wang Xiaohan , Liu Lanxiang 
Published: June 2021

BackgroundHistological grade is one of the most important prognostic factors of endometrial carcinoma (EC) and when selecting preoperative treatment methods, conducting accurate preoperative grading is of great significance.PurposeTo develop a magnetic resonance imaging (MRI) radiomics-based nomogram for discriminating histological grades 1 and 2 (G1 and G2) from grade 3 (G3) EC.MethodsThis was a retrospective study included 358 patients with histologically graded EC, stratified as 250 patients in a training cohort and 108 patients in a test cohort. T2-weighted imaging (T2WI), diffusion-weighted imaging (DWI) and a dynamic contrast-enhanced three-dimensional volumetric interpolated breath-hold examination (3D-VIBE) were performed via 1.5-Tesla MRI. To establish ModelADC, the region of interest was manually outlined on the EC in an apparent diffusion coefficient (ADC) map. To establish the radiomic model (ModelR), EC was manually segmented by two independent radiologists and radiomic features were extracted. The Radscore was calculated based on the least absolute shrinkage and selection operator regression. We combined the Radscore with carbohydrate antigen 125 (CA125) and body mass index (BMI) to construct a mixed model (ModelM) and develop the predictive nomogram. Receiver operator characteristic (ROC) and calibration curves were assessed to verify the prediction ability and the degree of consistency, respectively.ResultsAll three models showed some amount of predictive ability. Using ADC alone to predict the histological risk of EC was limited in both the cohort [area under the curve (AUC), 0.715; 95% confidence interval (CI), 0.6509–0.7792] and test cohorts (AUC, 0.621; 95% CI, 0.515–0.726). In comparison with ModelADC, the discrimination ability of ModelR showed improvement (Delong test, P &lt; 0.0001 for both the training and test cohorts). ModelM, established based on the combination of radiomic and clinical indicators, showed the best level of predictive ability in both the training (AUC, 0.925; 95% CI, 0.898–0.951) and test cohorts (AUC, 0.915; 95% CI, 0.863–0.968). Calibration curves suggested a good fit for probability (Hosmer–Lemeshow test, P = 0.673 and P = 0.804 for the training and test cohorts, respectively).ConclusionThe described radiomics-based nomogram can be used to predict EC histological classification preoperatively.
Sleep-Related Cognitive Processes and the Incidence of Insomnia Over Time: Does Anxiety and Depression Impact the Relationship?
Authors: Norell-Clarke Annika , Hagström Mikael , Jansson-Fröjmark Markus 
Published: June 2021

Aim: According to the Cognitive Model of Insomnia, engaging in sleep-related cognitive processes may lead to sleep problems over time. The aim was to examine associations between five sleep-related cognitive processes and the incidence of insomnia, and to investigate if baseline anxiety and depression influence the associations.Methods: Two thousand three hundred and thirty-three participants completed surveys on nighttime and daytime symptoms, depression, anxiety, and cognitive processes at baseline and 6 months after the first assessment. Only those without insomnia at baseline were studied. Participants were categorized as having or not having incident insomnia at the next time point. Baseline anxiety and depression were tested as moderators.Results: Three cognitive processes predicted incident insomnia later on. Specifically, more safety behaviors and somatic arousal at Time 1 increased the risk of developing insomnia. When investigating changes in the cognitive processes over time, reporting an increase of worry and safety behaviors also predicted incident insomnia. Depressive symptoms moderated the association between changes in worry and incident insomnia.Conclusion: These findings provide partial support for the hypothesis that cognitive processes are associated with incident insomnia. In particular, safety behaviors, somatic arousal, and worry increase the risk for incident insomnia. Preventative interventions and future research are discussed.