bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between different anticoagulation schemes of pregnant women with mechanical prosthetic heart valves and adverse fetal/neonatal outcomes
Authors: Zhao Ziwen , Yang Xi , Su Ying , Zhang Jun , Li Yanna , Liu Guiying 
Published: July 2021

Abstract
Pregnant women with mechanical prosthetic heart valves (MPHV) have adverse effects on the growth and development of the fetus. Data on MPHV pregnant women and their offspring from our hospital from 2014 to 2020 were retrospectively collected. There were 137 pregnancies, 96 in the warfarin group, 28 in the low-molecular-weight heparin (LMWH) group, 13 in the sequential treatment group. There are 27 cases of spontaneous abortion, 3 cases of stillbirth, 56 live births and 51 artificial abortion. The international normalized ratio (INR, P < 0.001), prothrombin time (PT, P = 0.014), activated partial thromboplastin time (APTT, P = 0.035) of the neonates in the warfarin group were increased compared with the other two groups. Multivariate Tobit regression analysis showed that the probability of spontaneous abortion/stillbirth was positively correlated with INR before delivery (OR 1.13, P = 0.009) and valve usage time (OR 1.13, P = 0.009). The probability of malformation was positively correlated with worse heart function level (OR 1.20, P = 0.025) and INR (OR 1.18, P = 0.011) before delivery. The offspring of MPHV pregnant women who continuously take warfarin have poor outcomes and a significantly higher risk of early bleeding. Prenatal consultations for MPHV women should be strengthened to avoid unplanned pregnancy.
Risk Factors Influencing Cerebral Venous Infarction After Meningioma Resection
Authors: cai qing , Wang Shoujie , Zheng Min , Wang Xuejiao , Liu Rong , Liu Liqin , Qin Huaizhou , Feng Dayun 
Published: July 2021

Abstract
Introduction Cerebral venous infarction is a serious complication after meningioma resection. To reduce the incidence of venous infarction, we identified risk factors for postoperative cerebral venous infarction after surgical resection of meningioma in patients with clinical symptoms.Methods The clinical and imaging data of 1127 patients with intracranial meningiomas who underwent resection in our hospital were retrospectively collected and analyzed. Cerebral venous infarction was evaluated by postoperative imaging and clinical manifestations. Univariate and multivariate analyses were performed to identify risk factors associated with cerebral venous infarction.Results Overall, 3.3% (37/1127) of patients experienced symptomatic cerebral venous infarction after meningioma resection. Multivariate analysis revealed superficial meningioma, moderate to severe peritumoral edema, peritumoral critical vein and WHO grade II-III as independent predictors of a postoperative cerebral venous infarction. After timely intervention, the symptoms were clearly alleviated in one month, and the prognosis was good, but injury to key veins could cause irreversible neurological disorders.Conclusions Intraoperative protection of veins is the primary way to prevent cerebral venous infarction. The present study identified several significant and independent risk factors for postoperative venous infarction, thereby enabling the identification of high-risk patients who require special attention during clinical and surgical management.
Incidence and Potential Risk Factors of Catheter Associated Thrombosis in Solid Tumors
Authors: Mateo Francisco José Pelegrín , Arias Asia Ferrández , González Lucía Gómez , Verdúguez Teresa Quintanar , Heredia Irene Belmonte , Justamante Jaime Belmonte , Gómez Pilar Calderón , García María Carmen García , Manzanera Matilde Miguel , Lescure Álvaro Rodríguez 
Published: July 2021

Abstract
PurposeCentral venous catheters (CVCs) have become common practice in oncology. Besides their benefits, as an invasive procedure, several complications are associated with them. Catheter associated thrombosis (CAT) is one of the most relevant due to their impact in quality of life and mortality, but the prothrombotic risk factors implied have been poorly assessed. The aim of the study is to evaluate the incidence of upper extremity deep vein thrombosis (UEDVT) associated to catheter use in patients with solid tumors. Secondary endpoints are to describe the population using CVCs and to evaluate potential risk factors of CAT. MethodsPatients diagnosed of solid tumors assisted at a tertiary level hospital between 2016 and 2019, and using CVCs were included.Results455 patients were enrolled. The incidence of CAT was 5.49% (25) in the whole population. 5.05% (23) was associated with PICC while 0.44% (2) was due to PORT use. Among the factors included in the univariate and multivariate regression models, age ≥50 years and PORT use were identified as protective factors related to the development of CAT.ConclusionCVCs remains a safe approach for the delivering of treatments in patients with solid tumors. Age ≤50 and use of PICC are risk factors for developing CAT. Larger prospective studies are needed to identify additional risk factors of CAT.
Expression of Cell Proliferation Regulatory Factors Bricd5, Tnfrsf21, Cdk1 Correlates With Expression of Clock Gene Cry1 in Testis of Hu Rams During Puberty
Authors: Huang Yongjie , Jiang Xun Ping , Liu Guiqiong , Liu Chen Hui 
Published: July 2021

Abstract
The cry1 gene plays an important role in mammalian ontogeny and widely exists in various tissues. Studies showed that cry1 gene was expressed in testis and participated in the regulation of mammalian reproductive activities. To identify the genes that are positively correlated with cry1 gene expression in the testis of rams during the first estrus, and to explore the relationship between cry1 gene and the number of spermatogenic cells. qRT-PCR was used to detect the mRNA transcription levels of cry1, bricd5, tnfrsf21, cdk1 and tcfl5 in testicular tissues of Hu sheep at 0, 30, 60, 90, 120, 150 and 180 days postpartum (dpp) . In testicular tissue, the expression of cry1 mRNA increased with age in testis showed an upward trend, and increased significantly in the estrous phase. The cry1 mRNA at 180dpp was significantly higher than that of 90-day-old testis (p < 0.05). The expression of cell proliferation related genes bricd5, tnfrsf21, cdk1, cry1 upstream specific transcription factor tcfl5 was identified, and the mRNA expression at 180 dpp was significantly higher than that of 3-month-old testis (p < 0.05) The expression level of cry1 mRNA was similar to that of cry1. cry1 gene is highly expressed in the testis of sheep during puberty, and has a significant correlation with the proliferation of spermatogenic cells.
The effect of early enteral nutrition on intestinal mucosal barrier and remote organs in rats with severe acute pancreatitis
Authors: Song Jianbo , Lu xiaoguang , Zhan Libin , Kang Xin , Zhong Yilong , Yang Yilun , Wang Yi , Guo Wenxiu , Song Yi 
Published: July 2021

Abstract
Background

The mechanism of early enteral nutrition on intestinal mucosal barrier is still not fully understood and the direct evidence to demonstrate the protective effects of early enteral nutrition against remote organs injury induced by severe acute pancreatitis is lacking.
Methods

Rats were randomly divided into three groups: 1) only flip pancreas and free diet (n = 8); 2) severe acute pancreatitis and total parenteral nutrition (n = 15); 3) severe acute pancreatitis and early enteral nutrition (n = 15). Severe acute pancreatitis was induced by the retrograde injection of pancreaticobiliary duct with sodium taurocholate. Nutritional supplementation was administered starting 12 hours after induction of pancreatitis. The samples were obtained after the treatment of nutrition 72 hours.
Results

The mortality was not significant difference between the experimental groups. The pathology of ileum and the content of serum diamine oxidase were significantly lower in early enteral nutrition group than total parenteral nutrition group. The expression of tight junction ZO-1, Occludin and Claudin-1 proteins and the intraepithelial CD8+ and TCRγδ+ lymphocytes were significantly higher in early enteral nutrition group than total parenteral nutrition group. The content of the serum amylase and endotoxin were significantly lower in early enteral nutrition group than total parenteral nutrition group. There was a tendency of decreased pancreas and lung pathology scores in early enteral nutrition group, but the difference between total parenteral nutrition and early enteral nutrition groups was not significant.
Conclusion

The early enteral nutrition could significantly improve the expression of the tight junctions and intraepithelial lymphocytes, accompanied by improved intestinal mucosal barrier and endotoxin, but failed to the pancreas and lung injury compared to total parenteral nutrition in the rat model of severe acute pancreatitis.
Na+/Ca2+ exchanger isoform 1 takes part to the Ca2+-related prosurvival pathway of SOD1 in primary motor neurons exposed to beta-methylamino-L-alanine
Authors: Secondo Agnese , Petrozziello Tiziana , Boscia Francesca , Tedeschi Valentina , Pannaccione Anna , de Rosa Valeria , Corvino Angela , Severino Beatrice , Annunziato Lucio 
Published: July 2021

Abstract
Background: The cycad neurotoxin beta-methylamino-L-alanine (L-BMAA), causing the amyotrophic lateral sclerosis/Parkinson-dementia complex (ALS/PDC), may cause neurodegeneration by disrupting organellar Ca2+ homeostasis. By activating Akt/ERK1/2 pathway, the Cu,Zn-superoxide dismutase (SOD1) and its non-metallated form, ApoSOD1, prevent endoplasmic reticulum (ER) stress-induced cell death in motor neurons exposed to LBMAA. This occurs through the rapid increase of intracellular Ca2+ concentration ([Ca2+]i) in part flowing from the extracellular compartment and in part released from ER. However, the molecular components of this mechanism remain uncharacterized.Methods: By an integrated approach consisting on the use of siRNA strategy, Western blotting, confocal double labeling immunofluorescence, patch-clamp electrophysiology, and Fura 2- /SBFI-single-cell imaging, we explored in rat motor neuron-enriched cultures the involvement of plasma membrane Na+/Ca2+ exchanger (NCX) and the purinergic P2X7 receptor as well as of the intracellular cADP-ribose (cADPR) pathway in the rapid and neuroprotective mechanism of SOD1.Results: we showed that SOD1-induced [Ca2+]i rise was prevented by the pan inhibitor of NCX CB-DMB but not by A430879, a P2X7 receptor specific antagonist, or by 8-bromo-cADPR, a cell permeant antagonist of cADP-ribose. The same occurred for the ApoSOD1. Confocal double labeling immunofluorescence showed a huge expression of plasmalemmal NCX1 and intracellular NCX3 isoforms. Furthermore, we identified NCX1 reverse mode as the main mechanism responsible for the neuroprotective ER Ca2+ refilling elicited by SOD1 and ApoSOD1. Furthermore, SOD1 and ApoSOD1 promoted translocation of active Akt in some nuclei of primary motor neurons. Finally, the activation of NCX1 by the specific agonist CNPYB2 protected motor neurons from L-BMAA-induced cell death.Conclusion: collectively, our data indicate that SOD1 and ApoSOD1 exert their neuroprotective effect by modulating ER Ca2+ content through the activation of NCX1 reverse mode and Akt nuclear translocation in a subset of primary motor neurons.
Population-based Study of Cutaneous Malignant Melanoma in the South Region of Portugal: Epidemiological and Clinical Characterization (2016-2017)
Authors: Costa Filipa Alves da , Bernardo Catarina , Borges Fábio Cardoso , Ramos Adriana , Miranda Ana Costa 
Published: July 2021

Abstract
An historical population-based cohort study was conducted aiming to estimate the incidence of cutaneous malignant melanoma in the South Region of Portugal between Jan 2016 and June 2017; to clinically characterize the diagnosed individuals; to describe instituted treatment; and to estimate survival outcomes. Data were extracted from a cancer registry (ROR-Sul) covering 4 800 000 inhabitants (46% of the Portuguese population) and included a total of 789 individuals meeting eligibility criteria. The cumulative incidence rate (18 months) of melanoma was 13.36/100,000 inhabitants and the Age-Standardized Incidence Rate per 100,000 World population was 8.43/100,000 inhabitants. The most common histological subtypes identified were superficial extension, followed by malignant melanoma and nodular melanoma. Most cases were diagnosed in stage I (50.39%), equally distributed by sex and with a median age of 65 years. During the study period, 174 recurrence events were recorded (23.45%) and recurrence-free survival rate was significantly lower in more advanced stages. Patients had a two-fold risk of recurrence/death when in presence of ulcerated tumors [adjusted hazard ratio (adj HR) = 2.28; 95% confidence interval (CI): 1.40–3.70]. Overall survival rate at 3-years was 80.54% (77.58–83.15) and survival was considerably higher for individuals diagnosed at earlier stages. The epidemiologic and clinical characteristics of malignant melanoma patients studied are consistent with international literature. Treatment instituted by Portuguese clinicians is aligned with evidence-based guidelines published. The increase in the incidence rate observed suggests additional public health campaigns are needed to modify behaviours of the Portuguese population and thus reduce their risk.
Diagnosis of De Novo Mosaic Balanced Translocation t(1;3)(q42;q25) in a Fetus Conceived Using Pre-implantation Diagnosis Due to Presence in the Father of a Different Balanced Translocation
Authors: Zhang Shaoqin , zhu jianjiang , Qi Hong , Xu Limei , Cai Lirong , Meng Ran 
Published: July 2021

Abstract
IntroductionPreimplantation genetic testing (PGT) had widely been applied in reciprocal translocation carriers to improve the clinical outcome of assisted reproduction. De novo mosaicism balanced reciprocal translocations in fetus conceived using PGT from a balanced translocation carrier parent has been rarely reported, and the driving mechanism is not clearly. MethodsChromosomal microarray analysis (CMA) , karyotype analysis and fluorescent in situ hybridization (FISH) were performed to verify the type and heredity of the rearrangement. STR analysis was used to identify potential contamination as well as kinship verification and identification. ResultsA rare de novo mosaicism balanced reciprocal translocation t(1,3)(q42;q25) in fetus conceived using PGT-SR from a t(12;14)(q22;q13) balanced translocation carrier father was been diagnosed by multiplatform genetic techniques. At 31 weeks and 2 days of gestation, premature delivery was caused by uncontrollable uterine contractions. At the 21-months follow up, infant has achieved all psychomotor development milestones as well as growth within the normal reference range. Conclusion﻿PGT cases still need close observation in prenatal diagnosis and long-term follow-up.
The Study of Ocular Parameters in Low to Moderate Myopic Chinese Children Age Between Eight to Eighteen-Year-Old
Authors: Li Zhengxuan , Sun Lu , Song Hongxin 
Published: July 2021

Abstract
Purpose: The primary aim of this study was to analyze the distribution of ocular parameters of children aged from eight to eighteen-year-old with low to moderate myopia as well as offer useful information for the design and clinical fitting of ortho-k lens in China.Methods: In this retrospective study, we collected data of 300 subjects (600 eyes) age between eight to eighteen-year-old from the myopic control outpatient clinic at Beijing Tongren Hospital, Beijing, China. The spherical equivalent (SE), axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), corneal endothelial cell density (CECD)，corneal diameter (CD), simulated K (Sim K) were collected from all the subjects and analyzed. Results: The mean of SE for all subjects was -3.12 ± 1.21D, the median was -3.16D. The mean of AL for all subjects was 24.72 ± 0.83 mm, the median was 24.72 mm. The mean of CCT for all subjects was 537.8 ± 29.82 μm, the median was 538.17 μm. The mean of CECD for all subjects was 3340.3 ± 352.6 cells/mm2, the median was 3357.77 cells/mm2. The mean of ACD for all subjects was 3.71 ± 0.21 mm, the median was 3.69 mm. The mean of CD for all subjects was 12.15 ± 1.67 mm, the median was 12.05 mm. The mean of flat K was 42.80 ± 1.29, the median was 42.81. The mean of steep K was 43.76 ± 1.37, the median was 43.76.Conclusions: This study provided the distribution of ocular parameters to offer useful information for diagnosis of many eye conditions. Meanwhile, the distribution of ocular parameters of children aged from eight to eighteen-year-old with low to moderate myopia is beneficial to make the application of overnight orthokeratology lens more appropriate and effective.
Factors Affecting Retention in the Philippine National Rural Physician Deployment Program From 2012 to 2019: A Mixed Methods Study
Authors: Flores Erika Louise L. , Manahan Edric Matthew R. , Lacanilao Miguel Paolo B. , Ladaw Isabella Ma. Beatriz T. , Mallillin Mico Martin B. , Mappatao Nikolai Thadeus Q. , Leonardia Juan Alfonso , Pepito Veincent Christian F. 
Published: July 2021

Abstract
BACKGROUND: To address the maldistribution of healthcare providers and the shortage of physicians in geographically isolated and disadvantaged areas of the Philippines, the Philippine National Rural Physician Deployment Program, or more commonly known as the Doctors to the Barrios (DTTB) program was established in 1993. However, as of 2011, only 18% of the DTTBs chose to stay in their assigned municipalities after their two-year deployment, termed retention. This study aims to identify the individual, local, work, national, and international factors affecting the retention of DTTBs in their assigned communities after their two-year deployment. METHODS: A descriptive, mixed-methods design was used. For the quantitative part, the modified and updated Stayers Questionnaire was given to all current DTTBs present in a Continuing Medical Education session in the Development Academy of the Philippines. Descriptive statistics were then presented. For the qualitative part, individual, semi-structured key informant interviews were conducted in-person or via phone with current and alumni DTTBs from 2012 to 2019. Proceedings of the interviews were transcribed, translated, and analyzed thematically. RESULTS: 102 current DTTBs participated in the quantitative part of our study, while 10 current and former DTTBs participated in the interviews. Demographic factors and location, personal beliefs, well-being, friends and family dynamics, and perceptions about work were the individual factors identified to affect retention. Social working conditions, career development, and infrastructure, medical equipment, and supplies were among the work factors identified to affect retention. Geography, living conditions, local social needs, and technology were among the local factors identified to affect retention. Compensation, the recently signed Universal Healthcare Law, and Safety and Security were identified as national factors that could affect retention. International factors did not seem to discourage DTTBs from staying in their communities. CONCLUSIONS: A host of individual, work-related, local, national, and international factors influence the DTTB’s decision to be retained in different, complex, interconnected, and dynamic ways. We also identified implementation issues in the DTTB program and suggested interventions to encourage retention.
The Brain’s Structural Differences in Peripheral Neuropathic Pain: A Comparison Study Between Postherpetic Neuralgia and Lower Back Pain
Authors: Qiu Jianxing , Du Mengjiao , Yang Junzhe , Lin Zengmao , Qin Naishan , Sun Xiaowei , Li Linling , Zou Rushi , Wei Juan , Wu Bing , Liu Jing , Zhang Zhiguo 
Published: July 2021

Abstract
The purpose is to explore the brain’s structural difference in local morphology and between-region networks between two types of peripheral neuropathic pain (PNP): postherpetic neuralgia (PHN) and lower back pain (LBP). A total of 54 participants including 38 LBP and 16 PHN patients were enrolled. The average pain scores were 7.6 and 7.5 for LBP and PHN. High-resolution structural T1 weighted images were obtained. Both grey matter volume (GMV) and morphological connectivity (MC) were extracted. An independent two-sample t-test with false discovery rate (FDR) correction was used to identify the brain regions where LBP and PHN patients showed significant GMV difference. Next, we explored the differences of MC network between LBP and PHN patients and detected the group differences in network properties by using the two-sample t-test and FDR correction. Compared with PHN, LBP patients had significantly larger GMV in temporal gyrus, insula and fusiform gyrus (p < 0.05). The LBP cohort had significantly stronger MC in the connection between right precuneus and left opercular part of inferior frontal gyrus (p < 0.05). LBP patients had significantly stronger degree in left anterior cingulate gyrus and left rectus gyrus (p < 0.05) while had significantly weaker degree than PHN patients in left orbital part of middle frontal gyrus, left supplementary motor area and left superior parietal lobule (p < 0.05). LBP and PHN patients had significant differences in the brain’s GMV, MC, and network properties, which implies that different PNPs have different neural mechanisms concerning pain modulation.
Both Baicalein and Gallocatechin Gallate Efficaciously Inhibit SARS-CoV-2 Replication by Targeting Mpro and Sepsis in Mice
Authors: Xiao Ting , Cui Mengqi , Zheng Caijuan , Zhang Peipei , Ren Shanfa , Bao Jiali , Gao Dandi , Sun Ronghao , Wang Ming , Lin Jianping , Zhang Liang , Li Mingjiang , Li Dongmei , Zhou Honggang , Yang Cheng 
Published: July 2021

Abstract
Severe acute syndrome coronavirus 2(SARS-CoV-2) caused the global pandemic of COVID-19 since December 2019. Although most of COVID-19’s patients are mild or common, most of the severe patients have sepsis caused by the cytokine storm, which greatly increases the case fatality rate. Moreover, there is no effective drug that can resist the novel coronavirus so far, so it’s urgent to develop antiviral drug for the SARS-CoV-2. In our research, we screened 29 compounds with a score lower than -6 from 35 flavonoid compounds by molecular docking. (-)-Gallocatechin gallate, (+)-Gallocatechin and Baicalein were identified to have potent inhibit activity with IC50 5.774±0.805μM, 13.14±2.081μM and 5.158±0.928μM by FRET assay. Subsequently, we conducted molecular docking experiments, which showed that (-)-Gallocatechin gallate, (+)-Gallocatechin and Baicalein were non-covalently bound to Mpro through π-π stacking and hydrogen bonds in the Cys145 catalytic site. We further evaluated the effect of (-)-Gallocatechin gallate and Baicalein on cytokine storm use a mouse model of sepsis. (-)-Gallocatechin gallate and Baicalein significant reduced sepsis severity based on weight, murine sepsis score and survival rate and reduced the inflammatory factors level such as TNF-α, IL-1α, IL-4 and IL-10. Overall, (-)-Gallocatechin gallate and Baicalein may be potential drugs for symptomatic treatment of COVID-19.