bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring Reproductive Health Decision Experiences and Preferences of Women With Pediatric-Onset Inflammatory Bowel Diseases
Authors: Picciarelli Zach , Stransky Olivia M , Leech Mary M , Michel Hilary K , Schwartz Marc , Kim Sandra C , Gray Whitney M , Kazmerski Traci M 
Published: December 2021

Abstract

Background
Women with inflammatory bowel diseases (IBDs), such as Crohn’s disease or ulcerative colitis, face several disease-specific concerns related to their reproductive health decisions. This study explored the reproductive health decision-making experiences and preferences of women with IBD to discover ways to improve this aspect of comprehensive care.

Methods
We recruited women ages 18–44 years with IBD to participate in individual, semistructured interviews exploring their experiences and attitudes toward parenthood, pregnancy, contraception, and family planning care. Two independent coders performed analysis using an inductive and deductive coding approach and identified key themes.

Results
Twenty-one women with IBD participated in interviews (average age 24.7 ± 5.9 years, range 18–43 years; average age of diagnosis 14.1 ± 2.0 years). We identified 4 key themes: (1) Nulliparous women who do not currently desire pregnancy appear to lack reproductive health knowledge; (2) Women with IBD lack clarity regarding the role IBD plays in contraceptive choice; (3) Related to pregnancy, women are concerned about the heredity of IBD, antepartum disease activity, and the safety of their current medications; (4) Women with IBD typically default to their reproductive health provider for reproductive health care and counseling, but they expect their gastroenterologist to initiate relevant reproductive health discussions with them and to provide information in the context of their disease.

Conclusions
Women have concerns about the effects of IBD on pregnancy, parenthood, and contraceptive choice; however, many have had limited or no discussion with their gastroenterologist about the topic.
Recombinant Adjuvanted Zoster Vaccine and Reduced Risk of Coronavirus Disease 2019 Diagnosis and Hospitalization in Older Adults
Authors: Bruxvoort Katia J , Ackerson Bradley , Sy Lina S , Bhavsar Amit , Tseng Hung Fu , Florea Ana , Luo Yi , Tian Yun , Solano Zendi , Widenmaier Robyn , Shi Meng , Van Der Most Robbert , Schmidt Johannes Eberhard , Danier Jasur , Breuer Thomas , Qian Lei 
Published: December 2021

Abstract

Background
Some vaccines elicit nonspecific immune responses that may protect against heterologous infections. We evaluated the association between recombinant adjuvanted zoster vaccine (RZV) and coronavirus disease 2019 (COVID-19) outcomes at Kaiser Permanente Southern California.

Methods
In a cohort design, adults aged ≥50 years who received ≥1 RZV dose before 1 March 2020 were matched 1:2 to unvaccinated individuals and followed until 31 December 2020. Adjusted hazard ratios (aHRs) and 95% confidence intervals (CIs) for COVID-19 outcomes were estimated using Cox proportional hazards regression. In a test-negative design, cases had a positive severe acute respiratory syndrome coronavirus 2 test and controls had only negative tests, during 1 March–31 December 2020. Adjusted odds ratios (aORs) and 95% CIs for RZV receipt were estimated using logistic regression.

Results
In the cohort design, 149 244 RZV recipients were matched to 298 488 unvaccinated individuals. The aHRs for COVID-19 diagnosis and hospitalization were 0.84 (95% CI, .81–.87) and 0.68 (95% CI, .64–.74), respectively. In the test-negative design, 8.4% of 75 726 test-positive cases and 13.1% of 340 898 test-negative controls had received ≥1 RZV dose (aOR, 0.84 [95% CI, .81–.86]).

Conclusions
RZV vaccination was associated with a 16% lower risk of COVID-19 diagnosis and 32% lower risk of hospitalization. Further study of vaccine-induced nonspecific immunity for potential attenuation of future pandemics is warranted.
Comparative analysis of in vitro dynamics and mechanisms of ceftolozane/tazobactam and imipenem/relebactam resistance development in Pseudomonas aeruginosa XDR high-risk clones
Authors: Gomis-Font María A. , Cabot Gabriel , López-Argüello Silvia , Zamorano Laura , Juan Carlos , Moyá Bartolomé , Oliver Antonio 
Published: December 2021

Abstract

Objectives
To analyse the dynamics and mechanisms of stepwise resistance development to ceftolozane/tazobactam and imipenem/relebactam in XDR Pseudomonas aeruginosa clinical strains.

Methods
XDR clinical isolates belonging to ST111 (main resistance mechanisms: oprD−, dacB−, CARB-2), ST175 (oprD−, ampR-G154R) and ST235 (oprD−, OXA-2) high-risk clones were incubated for 24 h in Müeller-Hinton Broth with 0.125–64 mg/L of ceftolozane + tazobactam 4 mg/L or imipenem + relebactam 4 mg/L. Tubes from the highest antibiotic concentration showing growth were reinoculated into fresh medium containing concentrations up to 64 mg/L for 7 consecutive days. Two colonies per strain from each of the triplicate experiments were characterized by determining the susceptibility profiles, whole genome sequencing (WGS), and in vitro fitness through competitive growth assays.

Results
Resistance development occurred more slowly and reached a lower level for imipenem/relebactam than for ceftolozane/tazobactam in all tested XDR strains. Moreover, resistance development to imipenem/relebactam remained low even for ST175 isolates that had developed ceftolozane/tazobactam resistance during therapy. Lineages evolved in the presence of ceftolozane/tazobactam showed high-level resistance, imipenem/relebactam hypersusceptibility and low fitness cost, whereas lineages evolved in the presence of imipenem/relebactam showed moderate (borderline) resistance, no cross-resistance to ceftolozane/tazobactam and high fitness cost. WGS evidenced that ceftolozane/tazobactam resistance was mainly caused by mutations in the catalytic centres of intrinsic (AmpC) or acquired (OXA) β-lactamases, whereas lineages evolved in imipenem/relebactam frequently showed structural mutations in MexB or in ParS, along with some strain-specific mutations.

Conclusions
Imipenem/relebactam could be a useful alternative for the treatment of XDR P. aeruginosa infections, potentially reducing resistance development during therapy.
Continuous seasonal monitoring of nitrogen and water content in lettuce using a dual phenomics system
Authors: Weksler Shahar , Rozenstein Offer , Ben Dor Eyal 
Published: December 2021

Abstract
The collection and analysis of large amounts of information on a plant-by-plant basis contributes to the development of precision fertigation and may be achieved by combining remote-sensing technology with high-throughput phenotyping methods. Here, lettuce plants (Lactuca sativa) were grown under optimal and suboptimal nitrogen and irrigation treatments from seedlings to harvest. A Plantarray system was used to calculate and log weights, daily transpiration, and momentary transpiration rates throughout the experiment. From 15 d after planting until experiment termination, the entire array of plants was imaged hourly (from 09.00 h to 14.00 h) using a hyperspectral moving camera. Three vegetation indices were calculated from the plants’ reflectance signal: red-edge chlorophyll index (RECI), photochemical reflectance index (PRI), and water index (WI), and combined treatments, physiological measurements, and vegetation indices were compared. RECI values differed significantly between nitrogen treatments from the first day of imaging, and WI values distinguished well-irrigated from drought-treated groups before detecting significant differences in daily transpiration rate. The PRI, calculated hourly during the drought-treatment phase, changed with the momentary transpiration rate. Thus, hyperspectral imaging might be used in growing facilities to detect nitrogen or water shortages in plants before their physiological response affects yields.
Phytolectin nanoconjugates in combination with standard antifungals curb multi-species biofilms and virulence of vulvovaginal candidiasis (VVC) causing Candida albicans and non-albicans Candida
Authors: Senthilganesh Jayasankari , Kuppusamy Shruthi , Durairajan Rubini , Subramaniyan Siva Bala , Veerappan Anbazhagan , Paramasivam Nithyanand 
Published: December 2021

Abstract

Vulvovaginal candidiasis (VVC) is a commonly occurring yeast infection caused by Candida species in women. Among Candida species, C. albicans is the predominant member that causes vaginal candidiasis followed by Candida glabrata. Biofilm formation by Candida albicans on the vaginal mucosal tissue leads to VVC infection and is one of the factors for a commensal organism to get into virulent form leading to disease. In addition to that, morphological switching from yeast to hyphal form increases the risk of pathogenesis as it aids in tissue invasion. In this study, jacalin, a phytolectin complexed copper sulfide nanoparticles (NPs) have been explored to eradicate the mono and mixed species biofilms formed by fluconazole-resistant C. albicans and C. glabrata isolated from VVC patients. NPs along with standard antifungals like micafungin and amphotericin B have been evaluated to explore interaction behavior and we observed synergistic interactions between them. Microscopic techniques like light microscopy, phase contrast microscopy, scanning electron microscopy, confocal laser scanning microscopy were used to visualize the inhibition of biofilm by NPs and in synergistic combinations with standard antifungals. Real-time PCR analysis was carried out to study the expression pattern of the highly virulent genes which are responsible for yeast to hyphal switch, drug resistance and biofilm formation upon treatment with NPs in combination with standard antifungals. The current study shows that lectin-conjugated NPs with standard antifungals might be a different means to disrupt the mixed species population of Candida spp. that causes VVC.

Lay Summary
The present study focuses on exploiting the high biding affinity between the cell surface glycans present in Candida cells and the plant lectin, Jacalin. Jacalin serves as a ‘Trojan Horse’ wherein the lectin-coupled nanoparticles show a high efficacy when compared with the unconjugated nanoparticles. The present approach also improves the anti-biofilm activity of the antifungal drugs against drug-resistant Candida strains.
Gravitational wave signals from 2D core–collapse supernova models with rotation and magnetic fields
Authors: Jardine Rylan , Powell Jade , Müller Bernhard 
Published: December 2021

ABSTRACT
We investigate the impact of rotation and magnetic fields on the dynamics and gravitational wave emission in 2D core–collapse supernova simulations with neutrino transport. We simulate 17 different models of $15\, {\rm M}_\odot$ and $39\, {\rm M}_\odot$ progenitor stars with various initial rotation profiles and initial magnetic fields strengths up to $10^{12}\, \mathrm{G}$, assuming a dipolar field geometry in the progenitor. Strong magnetic fields generally prove conducive to shock revival, though this trend is not without exceptions. The impact of rotation on the post-bounce dynamics is more variegated, in line with previous studies. A significant impact on the time-frequency structure of the gravitational wave signal is found only for rapid rotation or strong initial fields. For rapid rotation, the angular momentum gradient at the proto-neutron star surface can appreciably affect the frequency of the dominant mode, so that known analytic relations for the high-frequency emission band no longer hold. In case of two magnetorotational explosion models, the deviation from these analytic relations is even more pronounced. One of the magnetorotational explosions has been evolved to more than half a second after the onset of the explosion and shows a subsidence of high-frequency emission at late times. Its most conspicuous gravitational wave signature is a high-amplitude tail signal. We also estimate the maximum detection distances for our waveforms. The magnetorotational models do not stick out for higher detectability during the post-bounce and explosion phase.
Radiographic factors associated with painful callosities after forefoot surgery in patients with rheumatoid arthritis
Authors: Tobimatsu Haruki , Ikari Katsunori , Yano Koichiro , Okazaki Ken 
Published: December 2021

ABSTRACT

Objectives
Operative procedures for rheumatoid forefoot deformities have gradually changed from arthrodesis or resection arthroplasty to joint-preserving surgery. Although joint-preserving arthroplasty has yielded good outcomes, painful plantar callosities may occur post-operatively. This study aimed to reveal the radiographic factors associated with painful callosities after joint-preserving surgery for forefoot deformities in patients with rheumatoid arthritis (RA).

Methods
We retrospectively evaluated 166 feet in 133 patients with RA who underwent forefoot joint-preserving arthroplasty, including proximal rotational closing-wedge osteotomies of the first metatarsal, between January 2012 and December 2015. Logistic regression analysis was performed with the objective variable set as the presence/absence of painful plantar callosities at the final observation and the explanatory variables set as several radiographic factors, including post-operative relative first metatarsal length (RML), amount of dorsal dislocation of the fifth metatarsal (5DD), and arc failure of the lesser toes.

Results
At the final follow-up, 42 of the 166 feet (25.3%) had painful callosities under the metatarsal heads post-operatively. Logistic regression analysis showed that the RML, 5DD, and lesser toes’ arc failure were significantly associated with painful callosities.

Conclusions
We identified that RML, 5DD, and arc failure of the lesser toes were associated with painful plantar callosities after the surgery.
Associations of Smokeless Tobacco Use With Cardiovascular Disease Risk: Insights From the Population Assessment of Tobacco and Health Study
Authors: Rezk-Hanna Mary , Warda Umme Shefa , Stokes Andrew C , Fetterman Jessica , Li Jian , Macey Paul M , Darawad Muhammad , Song Yeonsu , Ben Taleb Ziyad , Brecht Mary-Lynn , Benowitz Neal L 
Published: December 2021

Abstract

Introduction
Cigarette smoking is strongly associated with the development of cardiovascular disease (CVD). However, evidence is limited as to whether smokeless tobacco (ST) use is associated with CVD.

Aims and Methods
Using data from 4347 adults in the Population Assessment of Tobacco and Health Study (2013–2014), we compared geometric mean concentrations of CVD-related harm biomarkers and biomarkers of exposure among exclusive ST users and exclusive cigarette smokers—in relation to recent nicotine exposure—and never tobacco users, adjusting for age, sex, race/ethnicity, income, body mass index, and CVD. Biomarker levels among exclusive ST users who were former established cigarette smokers were compared with exclusive cigarette smokers.

Results
Compared with cigarette smokers, ST users had significantly higher concentrations of total nicotine equivalents (TNE) but lower concentrations of inflammatory (high-sensitivity C-reactive protein, interleukin-6, intercellular adhesion molecule, fibrinogen) and oxidative stress (8-isoprostane) biomarkers (all p &lt; .05). Biomarker levels among ST users were similar to never smokers. ST users who were former cigarette smokers had lower levels of inflammatory and oxidative stress biomarkers and biomarkers of exposure (cadmium, lead, 1-hydroxypyrene, acrylonitrile, and acrolein), compared with cigarettes smokers (p &lt; .05), despite having higher TNE levels (p &lt; .05). Among cigarette smokers, but not among ST users, inflammatory biomarkers and TNE were highly correlated.

Conclusions
ST use is not associated with increases in biomarkers of CVD-related harm and exposure, compared with never smokers, despite exposure to nicotine at levels higher than those observed among cigarette smokers. These findings support the concept that increases in CVD risk among cigarette smokers is caused primarily by constituents of tobacco smoke other than nicotine.

Implications
Despite having higher levels of nicotine and compared with exclusive cigarette smokers, exclusive ST users (including those who were former cigarette smokers) had significantly lower concentrations of inflammatory and oxidative stress biomarkers, comparable to levels observed among never tobacco users. These findings suggest that increases in CVD risk among cigarette smokers is caused primarily by tobacco constituents other than nicotine and that switching to ST is likely associated with lower CVD risk.
Price and Income Elasticities of Cigarette Smoking Demand in Bangladesh: Evidence from Urban Adolescents and Young Adults
Authors: Ahmed Moin Uddin , Pulok Mohammad Habibullah , Hashmi Rubayyat , Hajizadeh Mohammad , Nargis Nigar 
Published: December 2021

Abstract

Introduction
Understanding the elasticities of cigarette smoking demand among the youth could help improve the effectiveness of tobacco control interventions. The objective of this study is to measure the price and income elasticities of cigarette smoking demand among urban Bangladeshi male adolescents and young adults aged 10-24 years.

Method
Using data from a cross-sectional survey conducted in seven urban districts of Bangladesh, we applied probit and ordinary least square (OLS) models to examine the effect of price and income on smoking participation (decision to smoke) and intensity (number of cigarettes smoked).

Results
Our results showed that price was not significantly associated with the decision to smoke, while income was a significant determinant of smoking participation. Both price and income determined the smoking intensity. The positive income elasticity (0.39) indicated that participants with greater access to money were more likely to participate in cigarette smoking and smoked more cigarettes. Negative price elasticity (-0.62) implied that increasing prices could lead to a reduction in smoking intensity among adolescents and young adults in urban Bangladesh.

Conclusion
The inelastic price demand for cigarette smoking suggests that there is scope for increasing tax on cigarettes without compromising the tax revenue.

Implications
This is the first study to investigate price and income elasticities among urban adolescents and young adults in Bangladesh. The study found no evidence that increasing the price of cigarettes discourages smoking participation but did show that increasing the price reduces the intensity of smoking among existing smokers. The results also suggest that economic measures such as taxation that increase the price of cigarettes could be a useful policy tool to limit smoking intensity without compromising government tax revenue.
Safety and Efficacy of Antibiotic De-escalation and Discontinuation in High-Risk Hematological Patients With Febrile Neutropenia: A Single-Center Experience
Authors: Verlinden Anke , Jansens Hilde , Goossens Herman , Anguille Sébastien , Berneman Zwi N , Schroyens Wilfried A , Gadisseur Alain P 
Published: December 2021

Abstract

Background
There is currently no consensus on optimal duration of antibiotic treatment in febrile neutropenia. We report on the clinical impact of implementation of antibiotic de-escalation and discontinuation strategies based on the Fourth European Conference on Infections in Leukaemia (ECIL-4) recommendations in high-risk hematological patients.

Methods
We studied 446 admissions after introduction of an ECIL-4–based protocol (hereafter “ECIL-4 group”) in comparison to a historic cohort of 512 admissions. Primary clinical endpoints were the incidence of infectious complications including septic shock, infection-related intensive care unit (ICU) admission, and overall mortality. Secondary endpoints included the incidence of recurrent fever, bacteremia, and antibiotic consumption.

Results
Bacteremia occurred more frequently in the ECIL-4 group (46.9% [209/446] vs 30.5% [156/512]; P &lt; .001), without an associated increase in septic shock (4.7% [21/446] vs 4.5% [23/512]; P = .878) or infection-related ICU admission (4.9% [22/446] vs 4.1% [21/512]; P = .424). Overall mortality was significantly lower in the ECIL-4 group (0.7% [3/446] vs 2.7% [14/512]; P = .016), resulting mainly from a decrease in infection-related mortality (0.4% [2/446] vs 1.8% [9/512]; P = .058). Antibiotic consumption was significantly reduced by a median of 2 days on antibiotic therapy (12 vs 14; P = .001) and 7 daily antibiotic doses (17 vs 24; P &lt; .001) per admission period.

Conclusions
Our results support implementation of ECIL-4 recommendations to be both safe and effective based on real-world data in a large high-risk patient population. We found no increase in infectious complications and total antibiotic exposure was significantly reduced.
Cofactor-independent RNA editing by a synthetic S-type PPR protein
Authors: Bernath-Levin Kalia , Schmidberger Jason , Honkanen Suvi , Gutmann Bernard , Sun Yueming Kelly , Pullakhandam Anuradha , Colas des Francs-Small Catherine , Bond Charles S , Small Ian 
Published: December 2021

Abstract
Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that are attractive tools for RNA processing in synthetic biology applications given their modular structure and ease of design. Several distinct types of motifs have been described from natural PPR proteins, but almost all work so far with synthetic PPR proteins has focused on the most widespread P-type motifs. We have investigated synthetic PPR proteins based on tandem repeats of the more compact S-type PPR motif found in plant organellar RNA editing factors and particularly prevalent in the lycophyte Selaginella. With the aid of a novel plate-based screening method, we show that synthetic S-type PPR proteins are easy to design and bind with high affinity and specificity and are functional in a wide range of pH, salt and temperature conditions. We find that they outperform a synthetic P-type PPR scaffold in many situations. We designed an S-type editing factor to edit an RNA target in E. coli and demonstrate that it edits effectively without requiring any additional cofactors to be added to the system. These qualities make S-type PPR scaffolds ideal for developing new RNA processing tools.