bluuebunny/crossref_metadata_2025_split huggingface dataset:
The utilisation solutions of olive mill by‐products in the terms of sustainable olive oil production: a review
Authors: Klisović Dora , Novoselić Anja , Režek Jambrak Anet , Brkić Bubola Karolina 
Published: May 2021

SummaryDespite the high economic and nutritional value as major positive aspects, olive oil production has an unfavourable side, which is the negative environmental impact caused by the generation of significant amounts of liquid and solid wastes. Therefore, the implementation of sustainable technologies to add value to the olive oil production process is a matter of great interest. This study aimed to review novel solutions dealing with the utilisation of olive oil production by‐products while taking into account sustainable waste management options. The most promising technologies for the production of high‐added value products from olive oil production by‐products have been described with a special attention to the sustainable nonthermal technologies for the extraction of bioactive phenolic compounds, and the key findings of such investigations were reviewed. All described technologies are environmentally friendly and show great potential. Nevertheless, further researches are required to optimise and increase their applicability.
Comparing learning ecologies of primary graphical programming: create or fix?
Authors: Neutens Tom , Barbion Evelien , Coolsaet Kris , wyffels Francis 
Published: May 2021

AbstractIn the last few years, programming, computational thinking, and robotics are more frequently integrated into elementary education. This integration can be done in many different ways. However, it is still unclear which teaching methods work in which situations. To provide some clarity in this area, we compared two methods of integrating programming into a primary robotics workshop for learners aged ten to twelve. In one method, students create programs from scratch; in the other, they start with a faulty program they have to fix. These teaching methods were evaluated using the framework of learning ecology, which provides a holistic framework for assessing complex learning environments. We identified different indicators of learning ecology and assessed our workshops using a mixed‐methods approach. Our results showed no difference between the groups on the intrinsic dimension of a learning ecology. However, on the experiential dimension, the learners in the create group scored better on all tests. Our results show the value of a multidimensional assessment of learning ecology to understand different teaching techniques. Additionally, the results provide us with important insights on how to integrate programming into a primary robotics curriculum enabling teachers to select better methods for teaching computing in their classroom.
Prevalence of hypertensive disorders during pregnancy in France (2010‐2018): The Nationwide CONCEPTION Study
Authors: Olié Valérie , Moutengou Elodie , Grave Clémence , Deneux‐Tharaux Catherine , Regnault Nolwenn , Kretz Sandrine , Gabet Amélie , Mounier‐Vehier Claire , Tsatsaris Vassilis , Plu‐Bureau Geneviève , Blacher Jacques 
Published: May 2021

AbstractHypertensive disorders of pregnancy (HDP) are one of the leading causes of maternal and fetal morbidity and mortality. We aimed to estimate the prevalence of each HDP in France and to study their associations. All pregnant women who delivered in France between 2010 and 2018 were included in a cohort and followed during their pregnancy and 6 weeks of postpartum. Each HDP occurring during the follow‐up was identified. Prevalence of each HDP and cumulative incidence by gestational age were estimated. Incidence rate ratio (IRR) and 95% confidence interval (CI) for preeclampsia among women with preexisting or gestational hypertension (GH) were estimated using Poisson regression and adjusted for age were estimated. Between 2010 and 2018, 6 302 810 deliveries were included. HDP complicated 7.4% of pregnancies. Preeclampsia and GH complicated 2.0% and 4.2% of pregnancies, respectively. Most of preeclampsia cases occurred without a prior HDP. HELLP syndrome represented 10.4% of preeclampsia cases. Compared to nulliparous pregnancies without HDP prior preeclampsia, the age‐adjusted IRR of preeclampsia was 6.2 [95% CI: 6.1‐6.4] in nulliparous pregnancies with preexisting hypertension and 2.9 [95% CI: 2.8‐3.0] in nulliparous pregnancies with GH. In France, HDP occurred in 7.4% of all pregnancies. Women with preexisting chronic hypertension are at high risk to present preeclampsia during pregnancy. Preeclampsia complicated 2.0% of pregnancies in France. Tailoring management of women according to the HDP is a major challenge to avoid complications related to these disorders.
Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection
Authors: Prasitporn Terawut , Senapin Saengchan , Vaniksampanna Akapon , Longyant Siwaporn , Chaivisuthangkura Parin 
Published: May 2021

AbstractScale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil‐DNA glycosylase (UDG)‐supplemented cross‐priming amplification (UCPA) combined with a colorimetric detection method using the hydroxynaphthol blue (HNB) and lateral flow dipstick (LFD) for detection of SDDV was developed. The UDG was utilized to prevent carryover contamination, and the CPA reactions can be readily observed by HNB and LFD. The CPA primers and probe were designed to target the major capsid protein (MCP) gene of the SDDV. The optimized UCPA conditions were performed at the temperature of 61°C for 60 min. The UCPA assays demonstrated specificity to SDDV without cross‐reaction to other tested viruses including red‐spotted grouper nervous necrosis virus (RGNNV), infectious spleen and kidney necrosis virus (ISKNV) and Lates calcarifer herpes virus (LCHV), and other bacterial species commonly found in aquatic animals. The sensitivity of the UCPA‐HNB and UCPA‐LFD was 100 viral copies/µl and 10 pg of extracted total DNA, which was 10‐fold more sensitive than that of conventional PCR. The UCPA‐HNB and UCPA‐LFD assays could be used to detect the SDDV infection in all 25 confirmed SDDV‐infected fish samples. Therefore, the UCPA coupled with HNB and LFD was rapid, simple and effective and might be applied for diagnosis of SDDV infection.
Rural disparities in end‐of‐life care for patients with heart failure: Are they due to geography or socioeconomic disparity?
Authors: Hutchinson Rebecca N. , Han Paul K. J. , Lucas F. Lee , Black Adam , Sawyer Douglas , Fairfield Kathleen 
Published: May 2021

AbstractPurposeThe impact of rurality and socioeconomic deprivation on end‐of‐life (EOL) care for patients with heart failure (HF) is unknown. We analyzed claims to describe the prevalence and predictors of EOL health care utilization for patients dying with HF in a predominantly rural state.MethodsWe used the MaineHealth Data Organization's All‐Payer Claims Data to identify 15,168 patients ≥35 who died with HF between 2012 and 2017. The primary outcome was health care utilization during the last 180 days of life (EOL definition for this analysis), including emergency department (ED) visits, hospitalizations, intensive care unit (ICU) admissions, and hospice utilization. Patient characteristics analyzed included age, gender, comorbidities, area deprivation index (ADI), and rurality.FindingsAmong 15,168 patients ≥35 who died with HF, 48% had ≥2 hospitalizations, 72% had ≥2 ED visit, 29% had an ICU stay, 2% initiated dialysis during EOL, and 64% received hospice. Rural patients were more likely to have an ICU admission and have ≥2 hospitalizations. Patients residing in areas with higher ADI were more likely to be hospitalized, admitted to the ICU, and started on dialysis. Both rural patients and those living in higher ADI areas were less likely to receive hospice. After multivariable adjustment, rurality and ADI were independently associated with a decreased likelihood of receiving hospice (OR 0.62 [95% CI: 0.53‐0.72] for the most rural patients and OR 0.64 [95% CI: 0.57‐0.72] for the highest ADI).ConclusionBoth rurality and local area deprivation drive disparities in EOL care for patients dying with heart failure.
Inverted V‐shaped extracapsular stabilisation technique and arthroscopic findings in six dogs with medial shoulder instability
Authors: Hammer M. , Grand J. G. 
Published: May 2021

ObjectivesThe aim of this case series was to report arthroscopic findings and to evaluate clinical outcomes of dogs treated with an inverted V‐shaped extracapsular stabilisation technique without additional external coaptation for medial shoulder instability.Material and MethodsMedical records of dogs with medial shoulder instability that underwent arthroscopy and an inverted V‐shaped extracapsular stabilisation technique were retrospectively reviewed. Extracapsular stabilisation was performed in all dogs using screws and spiked washers as anchor points for the coated polyester prosthetic ligament. No additional external coaptation was used in any dog. A minimum 18‐month follow‐up period was required for study inclusion. Complications and long‐term outcomes were recorded.ResultsThe subscapularis tendon and medial glenohumeral ligament were the structures most commonly affected. All dogs had pathological cartilage fibrillations. Implants were successfully placed in all dogs. Implant loosening was recorded in two out of six dogs on recheck but no revision surgery was performed. Function at the time of last clinical follow‐up (median, 28 months, range 18 to 33 months) was full in four out of six and acceptable in two out of six dogs.
Clinical SignificanceSurgical treatment of medial shoulder instability using the novel inverted V‐shaped extracapsular stabilisation technique was successful. Potential complications such as implant loosening may occur. Despite the limited number of cases, the present study suggests that additional postoperative external coaptation may not be necessary for successful management of dogs with medial shoulder instability.
A review on cobia, Rachycentron canadum, aquaculture
Authors: Benetti Daniel D. , Suarez Jorge , Camperio Julio , Hoenig Ronald H. , Tudela Carlos E. , Daugherty Zack , McGuigan Charles J. , Mathur Shubham , Anchieta Luiz , Buchalla Yole , Alarcón Jorge , Marchetti Dario , Fiorentino Julian , Buchanan John , Artiles Adriana , Stieglitz John D. 
Published: May 2021

AbstractCobia, Rachycentron canadum, is an important species for aquaculture worldwide. Production technology from egg to market was established in the early 1990s and continues to be perfected to this day. This species exhibits extraordinary scope for growth and can reach between 4 and 8 kg in 1 year, with females growing almost twice as fast and large as males. Despite continuous progress in maturation, spawning, larval rearing, fingerling production, nutrition, health management, genetics, and growout technology, overall cobia aquaculture production worldwide has been slow in the last decade. One of the biggest challenges remains the development of practical commercial feeds that are ecologically and economically efficient for this species. Feed conversion ratios are still very high, ranging from 2.0 to 3.0:1. In addition to nutritional challenges, diseases such as Photobacterium, Amyloodinium ocellatus, and Brooklynella hostilis continue to impact cobia aquaculture production worldwide. Genetics and breeding programs for cobia are still at their infancy. We report on current status of cobia breeding efforts as well as on advances on developing female monosex populations to exploit the sexually dimorphic growth in this species. Nutrition, health, and genetics will be the greatest drivers to improve overall performance and increases in production of the cobia aquaculture industry.
Individual characteristics and community context in decisions to divert or arrest
Authors: Nadel Melissa R. , Pesta George , Blomberg Thomas , Bales William D. 
Published: May 2021

AbstractDiversion programs are increasingly being implemented as an alternative to more severe sanctions, especially within juvenile justice. The civil citation program in Florida is unique in that it diverts juveniles away from the justice system at the earliest decision point of arrest. However, despite its growing use in a number of states, there is little research on the program's implementation and outcomes, namely, it remains unknown if the program is being applied consistently across communities and for juveniles within those communities. Drawing from the larger sociology of punishment, race, and social control literature, and the associated theories of labeling and social threat, this study employs statewide data from the Florida Department of Juvenile Justice to explore the community and individual determinants of civil citation's use. Consistent with the theoretical arguments of labeling and social threat, the multilevel analysis finds that community and individual characteristics, in particular race, impact the likelihood of receiving civil citation. Implications for future research, theory, and policy are discussed.
On the use of genome‐wide data to model and date the time of anthropogenic hybridisation: An example from the Scottish wildcat
Authors: Howard‐McCombe Jo , Ward Daniel , Kitchener Andrew C. , Lawson Daniel , Senn Helen V. , Beaumont Mark 
Published: May 2021

AbstractWhile hybridisation has long been recognised as an important natural phenomenon in evolution, the conservation of taxa subject to introgressive hybridisation from domesticated forms is a subject of intense debate. Hybridisation of Scottish wildcats and domestic cats is a good example in this regard. Here, we developed a modelling framework to determine the timescale of introgression using approximate Bayesian computation (ABC). Applying the model to ddRAD‐seq data from 129 individuals, genotyped at 6546 loci, we show that a population of wildcats genetically distant from domestic cats is still present in Scotland. These individuals were found almost exclusively within the captive breeding programme. Most wild‐living cats sampled were introgressed to some extent. The demographic model predicts high levels of gene‐flow between domestic cats and Scottish wildcats (13% migrants per generation) over a short timeframe, the posterior mean for the onset of hybridisation (T1) was 3.3 generations (~10 years) before present. Although the model had limited power to detect signals of ancient admixture, we found evidence that significant recent hybridisation may have occurred subsequent to the founding of the captive breeding population (T2). The model consistently predicts T1 after T2, estimated here to be 19.3 generations (~60 years) ago, highlighting the importance of this population as a resource for conservation management. Additionally, we evaluate the effectiveness of current methods to classify hybrids. We show that an optimised 35 SNP panel is a better predictor of the ddRAD‐based hybrid score in comparison with a morphological method.
An HD‐ZIP‐MYB complex regulates glandular secretory trichome initiation in Artemisia annua
Authors: Xie Lihui , Yan Tingxiang , Li Ling , Chen Minghui , Hassani Danial , Li Yongpeng , Qin Wei , Liu Hang , Chen Tiantian , Fu Xueqing , Shen Qian , Rose Jocelyn K. C. , Tang Kexuan 
Published: May 2021

Summary

Plant glandular secretory trichomes (GSTs) produce various specialized metabolites. Increasing GST density represents a strategy to enhance the yield of these chemicals; however, the gene regulatory network that controls GST initiation remains unclear.

In a previous study of Artemisia annua L., we found that a HD‐ZIP IV transcription factor, AaHD1, promotes GST initiation by directly regulating AaGSW2. Here, we identified two AaHD1‐interacting transcription factors, namely AaMIXTA‐like 2 (AaMYB16) and AaMYB5.

Through the generation and characterization of transgenic plants, we found that AaMYB16 is a positive regulator of GST initiation, whereas AaMYB5 has the opposite effect. Notably, neither of them regulates GST formation independently. Rather, they act competitively, by interacting and modulating AaHD1 promoter binding activity. Additionally, the phytohormone jasmonic acid (JA) was shown to be associated with the AaHD1‐AaMYB16/AaMYB5 regulatory network through transcriptional regulation via a JASMONATE‐ZIM DOMAIN (JAZ) protein repressor.

These results bring new insights into the mechanism of GST initiation through regulatory complexes, which appear to have similar functions in a range of vascular plant taxa.
A case report of PHF6 mosaicism: Beyond the classic Börjeson‐Forssman‐Lehmann syndrome
Authors: Garcia‐Melendo Cristina , Roé Esther , Rodríguez‐Santiago Benjamín , Amat‐Samaranch Victòria , Cubiró Xavier , Puig Lluís , Boronat Susana 
Published: May 2021

AbstractWe report a 6‐year‐old female with linear skin hyperpigmentation on the axillae and groin, intellectual disability, dysplastic teeth and nails, and facial dysmorphism who was diagnosed with a novel PHF6 pathogenic splicing variant. Males with PHF6 mutations have been associated with the X‐linked recessive disorder Börjeson‐Forssman‐Lehmann, but females have a distinct phenotype which is likely modulated by X‐inactivation.
Sexual and Reproductive Health Literacy, Misoprostol Knowledge and Use of Medication Abortion in Lagos State, Nigeria: A Mixed Methods Study
Authors: Väisänen Heini , Moore Ann M. , Owolabi Onikepe , Stillman Melissa , Fatusi Adesegun , Akinyemi Akanni 
Published: May 2021

AbstractLittle is known about the link between health literacy and women's ability to safely and successfully use misoprostol to self‐induce an abortion. While abortion is only allowed to save a woman's life in Nigeria, misoprostol is widely available from drug sellers. We interviewed 394 women in 2018 in Lagos State, Nigeria, who induced abortion using misoprostol obtained from a drug seller to determine their sexual and reproductive health literacy (SRHL) and misoprostol knowledge levels; and how these were associated with ending the pregnancy successfully or seeking care for (perceived) complications. Our results show that women's misoprostol knowledge (measured both quantitatively and qualitatively) was low, but that almost all women were nevertheless able to use the drug effectively and safely. Higher SRHL was associated with being more likely to end the pregnancy successfully and also seeking postabortion health care. Our study is the first to examine this association and adds to the scarce literature examining the relationship between health literacy and self‐use of misoprostol to induce abortions in restrictive settings.
Evaluation of an improved rapid bacterial assay with untreated and pathogen‐reduced platelets: Detection of Acinetobacter strains
Authors: LaVerda David , Shinefeld Lisa , Best Nancy , Lisitu Johny , Tambolleo Gary , Vallejo Yli Remo 
Published: May 2021

AbstractBackgroundThe PGDprime® test was updated to enable Acinetobacter spp. detection to respond to morbidity and mortality events in 2018 and 2020 involving platelets contaminated with Acinetobacter‐calcoaceticus‐baumannii complex (ACBC). In one morbidity event, the first‐generation PGD test failed to detect ACBC. In two other reported events, pathogen‐reduced (PR) platelets contaminated with ACBC and other bacteria led to patient morbidity and one death.Study Design and MethodsA polyclonal antibody to Acinetobacter was integrated in the test device and evaluated for detection of Acinetobacter spp., including the ACBC isolate recovered in one of the 2018 contamination events. Limits of Detection for various Acinetobacter strains were determined in dilution studies. Detection of Acinetobacter growing in platelets after an initial low inoculum was evaluated. Use of the updated test as a secondary test after pathogen reduction was also evaluated by testing at 12‐h intervals PR platelet units inoculated with low levels of the 3 species reported in the fatal PR platelet: ACBC, Staphylococcus saprophyticus, and Leclercia adecarboxylata.ResultsThe test detected several Acinetobacter strains at the clinically relevant CFU/ml levels associated with septic transfusions and successfully detected Acinetobacter growing in various non‐PR platelet types after an initial low inoculum. In PR platelets, the test yielded a positive result with the 3 implicated bacteria in 48 h or less after inoculation, or 48–72 h earlier than the reported time of transfusion of contaminated PR platelets.ConclusionPGDprime was improved to detect Acinetobacter and has shown utility to interdict contaminated PR platelets.
Identifying the Influential Factors Enriching the Experiences of Visitors of Touristic Religious Sites: The Case Study of Al-Khandaq Battle Site
Authors: Amer Mohamed Saleh , Karban Abdullah Saeed , Majid Mohammad Rafee , Majrashi Abdulrahman Abdulaziz , Saleh Abba 
Published: May 2021

Enriching visitors' experiences at religious, historical sites (RHS) is getting more attention than before. This research aims to identify and investigate the influential factors enriching the visitors' experience at such a site, using Al-Khandaq battle site as the case study. Forty-two respondents were interviewed at the site and NVivo 12 pro software was used to code and analyze the responses. The study explored tourist motivations, expectations, and perceptions as the guiding constructs in detailed qualitative analysis. Foremost, learning, reliving the experience of the events of the battle, and Islamic values were the primary motivation to the historic site visitation. Next, findings reveal the extent to which tourists' expectations can be met through the availability of an open museum, accessibility, and the spatial and experiential simulation of the ancient Al-Khandaq path. Lastly, the need to reconstruct the historical site in spatial planning and experiential dimensions formed the main thrust of the element of visitors' perception. Based on the foregoing, the study recommends that Al-Khandaq site should be developed traditionally as an open museum, displaying both religious and historical elements of the battle with emphasis on the visual connection to the Prophet's mosque