bluuebunny/crossref_metadata_2025_split huggingface dataset:
К вопросу актуальности работ по установлению границ зон затопления и подтопления (на примере территории города Новосибирска)
Authors: Дубровский Алексей Викторович , Скоринская Елена Андреевна 
Published: November 2021

В Российской Федерации только начинаются работы по установлению зон затопления
и подтопления. Законодательно установлено внесение сведений о границах этих зон в единый государственный реестр недвижимости. Однако, при этом существует ряд нормативно-правовых и технологических вопросов, которые не решены и работы по установлению зон затопления и подтопления не выполнены в полном объеме. В статье дано описание основных руководящих нормативных документов, а также показаны новшества, которые внесли данные законы для усовершенствования процедуры установления зон затопления и подтопления.
Folic Acid–Modified miR-491-5p–Loaded ZIF-8 Nanoparticles Inhibit Castration-Resistant Prostate Cancer by Regulating the Expression of EPHX1
Authors: Ju Guanqun , Liu Bing , Ji Mingfei , Jin Rui , Xu Xiaojian , Xiao Yongshuang , Li Jie , Xu Dongliang , Huang Yuhua , Hou Jianquan 
Published: November 2021

Epoxide hydrolase 1 (EPHX1) has been reported to be related to the development of several tumors. However, the regulation of castration-resistant prostate cancer (CRPC) development by EPHX1 has not been reported. We used proteomic technology and found that the EPHX1 protein was highly expressed in CRPC tissues and the CRPC cell line C4-2. We performed screening and found that EPHX1 is a direct target of miR-491-5p. High miR-491-5p expression significantly reduced the EPHX1 level in C4-2 cells and inhibited C4-2 cell proliferation and migration. Zeolite imidazolate framework-8 (ZIF-8) has good thermal stability, a simple synthesis method, tumor site stability, and specific acid responsiveness. We synthesized ZIF-8 nanodrug vectors to deliver miR-491-5p into C4-2 cells. After loading miR-491-5p into ZIF-8, we modified the ZIF-8 surface with folic acid (FA) as the target group (FA@ZIF-8). Our synthesized nanodrug carrier showed less cytotoxicity to C4-2 cells even at 200 μg/ml. Modified FA could increase the efficiency of nanomaterial entry into C4-2 cells. FA@miR-491-5p@ZIF-8 could stably release miR-491-5p for a long period in both phosphate-buffered saline (pH 7.4) and acetate buffer (pH 4.8), and miR-491-5p was released faster at the beginning of the experiment in acetate buffer (pH 4.8). FA@miR-491-5p@ZIF-8 significantly reduced C4-2 cell proliferation and migration, and FA@miR-491-5p@ZIF-8 had a better effect than miR-491-5p alone. In vivo, FA@miR-491-5p@ZIF-8 significantly inhibited CRPC growth in nude mice. Overall, we verified that miR-491-4p regulated CRPC development by targeting EPHX1. The drug nanocarrier FA@miR-491-5p@ZIF-8 not only significantly reduced C4-2 CRPC cell proliferation and migration but also significantly inhibited CRPC growth. Our research provides a theoretical basis for treatment and treatment strategies for CRPC.
Association Between Insulin-like Growth Factor-1 rs35767 Polymorphism and Type 2 Diabetes Mellitus Susceptibility: A Meta-Analysis
Authors: Zeng Qiaoli , Zou Dehua , Zeng Qiaodi , Chen Xiaoming , Wei Yue , Guo Runmin 
Published: November 2021

Background: Insulin-like growth factor-1 (IGF-1) has been demonstrated to increase fatty acid β oxidation during fasting, and play an important role in regulating lipid metabolism and type 2 diabetes mellitus (T2DM). The rs35767 (T &gt; C) polymorphism, a functional SNP was found in IGF-1 promoter, which may directly affect IGF-1 expression. However, the inconsistent findings showed on the IGF-1 rs35767 polymorphism and T2DM risk.Methods: We performed a comprehensive meta-analysis to estimate the association between the IGF-1 rs35767 and T2DM risk among four genetic models (the allele, additive, recessive and dominant models).Results: A total 49,587 T2DM cases and 97,906 NDM controls were included in the allele model, a total 2256 T2DM cases and 2228 NDM controls were included in the other three genetic models (the additive; recessive and dominant models). In overall analysis, the IGF-1 rs35767 was shown to be significantly associated with increased T2DM risk for the allele model (T vs. C: OR = 1.251, 95% CI: 1.082–1.447, p = 0.002), additive model (homozygote comparisons: TT vs. CC: OR = 2.433, 95% CI: 1.095–5.405, p = 0.029; heterozygote comparisons: TC vs. CC: OR = 1.623, 95% CI: 1.055–2.495, p = 0.027) and dominant model (TT + CT vs. CC: OR = 1.934, 95% CI: 1.148–3.257, p = 0.013) with random effects model. After omitting Gouda’s study could reduce the heterogeneity, especially in the recessive model (TT vs. CC + CT: I2 = 38.7%, p = 0.163), the fixed effects model for recessive effect of the T allele (TT vs. CC + CT) produce results that were of borderline statistical significance (OR = 1.206, 95% CI: 1.004–1.448, p = 0.045). And increasing the risk of T2DM in Uyghur population of subgroup for the allele model.Conclusion: The initial analyses that included all studies showed statistically significant associations between the rs35767 SNP and type 2 diabetes, but after removing the Gouda et al. study produced results that were mostly not statistically significant. Therefore, there is not enough evidence from the results of the meta-analysis to indicate that the rs35767 SNP has a statistically significant association with type 2 diabetes.
Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Peripheral Mononuclear Cells in Patients With Ankylosing Spondylitis
Authors: Xu Huixuan , Yu Haiyan , Liu Lixiong , Wu Hongwei , Zhang Cantong , Cai Wanxia , Hong Xiaoping , Liu Dongzhou , Tang Donge , Dai Yong 
Published: November 2021

ObjectiveGenetic studies on ankylosing spondylitis (AS) have identified more than 100 pathogenic genes. Building a bridge between these genes and biologically targeted therapies is the current research hotspot.MethodsWe integrated single-cell assaying transposase-accessible chromatin sequencing (scATAC-seq) and single-cell RNA sequencing (scRNA-seq) to explore the key genes and related mechanisms associated with AS pathogenesis.ResultsWe identified 18 cell types in peripheral mononuclear cells from patients with AS and normal controls and summarized the cell-type-specific abnormal genes by scRNA-seq. Interestingly, we found that the pathogenic gene NFKB involved in AS progression originated from CD8+ T cells. Moreover, we observed an abnormal tumor TNF pathway mediated by abnormal expression of TNF, NFKB, FOS, JUN, and JUNB, and scATAC-seq results confirmed the abnormal accessible binding sites of transcriptional factors FOS, JUN, and JUNB. The final magnetic bead sorting and quantitative real-time PCR(RT-qPCR) confirmed that NFKB, FOS, JUN, and JUNB in CD8+ T cells differed in the AS group.ConclusionsOur results revealed a possible mechanism by which NFKB abnormally regulates FOS, JUN, and JUNB and drives AS progression, providing a novel perspective from a single cell point of view in AS.
Molecular Surveillance of Carbapenem-Resistant Gram-Negative Bacteria in Liver Transplant Candidates
Authors: Schultze Tilman G. , Ferstl Philip G. , Villinger David , Hogardt Michael , Bechstein Wolf O. , Göttig Stephan , Wichelhaus Thomas A. , Zeuzem Stefan , Trebicka Jonel , Waidmann Oliver , Welker Martin-Walter , Kempf Volkhard A. J. 
Published: November 2021

Background: Carbapenem-resistant Gram-negative bacteria (CRGN) cause life-threatening infections due to limited antimicrobial treatment options. The occurrence of CRGN is often linked to hospitalization and antimicrobial treatment but remains incompletely understood. CRGN are common in patients with severe illness (e.g., liver transplantation patients). Using whole-genome sequencing (WGS), we aimed to elucidate the evolution of CRGN in this vulnerable cohort and to reconstruct potential transmission routes.Methods: From 351 patients evaluated for liver transplantation, 18 CRGN isolates (from 17 patients) were analyzed. Using WGS and bioinformatic analysis, genotypes and phylogenetic relationships were explored. Potential epidemiological links were assessed by analysis of patient charts.Results: Carbapenem-resistant (CR) Klebsiella pneumoniae (n=9) and CR Pseudomonas aeruginosa (n=7) were the predominating pathogens. In silico analysis revealed that 14/18 CRGN did not harbor carbapenemase-coding genes, whereas in 4/18 CRGN, carbapenemases (VIM-1, VIM-2, OXA-232, and OXA-72) were detected. Among all isolates, there was no evidence of plasmid transfer-mediated carbapenem resistance. A close phylogenetic relatedness was found for three K. pneumoniae isolates. Although no epidemiological context was comprehensible for the CRGN isolates, evidence was found that the isolates resulted of a transmission of a carbapenem-susceptible ancestor before individual radiation into CRGN.Conclusion: The integrative epidemiological study reveals a high diversity of CRGN in liver cirrhosis patients. Mutation of carbapenem-susceptible ancestors appears to be the dominant way of CR acquisition rather than in-hospital transmission of CRGN or carbapenemase-encoding genetic elements. This study underlines the need to avoid transmission of carbapenem-susceptible ancestors in vulnerable patient cohorts.
Adolescents With Cancer Need Trustworthy Information and Prefer to Receive It From a Human Source Rather Than From the Internet: A Qualitative Study
Authors: Schwartz-Attias Irit , Raz Haya , Natanzon-Bracha Tamar , Finkelstein Adi , Kreitler Shulamith 
Published: November 2021

Background: In pediatric cancer, the legal obligation to provide information is usually toward the parents who are the authorized signatories of the informed consent form. It is now known that aside from providing information to the parents, it is also very important to provide information to the children and adolescents themselves. The question is how the adolescents relate to this. What information do they already possess and what would they like to know? Would they wish to hear the truth in all situations and at what stage? What are their preferred sources of information?Method: A qualitative study that included in-depth interviews with 19 adolescents with cancer, aged 8.5–18, who were receiving active treatments and had been diagnosed at least 1 month previously. The interviews were guided by 15 open-ended questions.Findings: The analysis of the interviews indicated that adolescents know quite a lot about the course of their disease and the information they lack is mainly etiological. The participants reported a lack of knowledge concerning sexuality and a sense of discomfort talking about it, leaving them with open questions. They all claimed that it is important to tell the truth: “Even if the truth is difficult, it is important to tell it.” The participants reported that information can be scary, so it must be structured and adapted to the age and emotional readiness of the individual. Most of the participants prefer not to use the internet as an information resource due to the profusion of stressful and non-adapted information.Conclusion: Adolescents with cancer need trustworthy information and prefer to receive it from a human source rather than from the internet. Not telling the truth can lead them to feel fear and loneliness. The medical staff must operate in sensitive and creative ways to provide adolescents with access to information on various subjects, including sexuality, which they are ashamed to talk about, leaving them with a sense of shame and a lack of knowledge in this area.
1,800 MHz Radiofrequency Electromagnetic Irradiation Impairs Neurite Outgrowth With a Decrease in Rap1-GTP in Primary Mouse Hippocampal Neurons and Neuro2a Cells
Authors: Li Yanqi , Deng Ping , Chen Chunhai , Ma Qinlong , Pi Huifeng , He Mindi , Lu Yonghui , Gao Peng , Zhou Chao , He Zhixin , Zhang Yanwen , Yu Zhengping , Zhang Lei 
Published: November 2021

Background: With the global popularity of communication devices such as mobile phones, there are increasing concerns regarding the effect of radiofrequency electromagnetic radiation (RF-EMR) on the brain, one of the most important organs sensitive to RF-EMR exposure at 1,800 MHz. However, the effects of RF-EMR exposure on neuronal cells are unclear. Neurite outgrowth plays a critical role in brain development, therefore, determining the effects of 1,800 MHz RF-EMR exposure on neurite outgrowth is important for exploring its effects on brain development.Objectives: We aimed to investigate the effects of 1,800 MHz RF-EMR exposure for 48 h on neurite outgrowth in neuronal cells and to explore the associated role of the Rap1 signaling pathway.Material and Methods: Primary hippocampal neurons from C57BL/6 mice and Neuro2a cells were exposed to 1,800 MHz RF-EMR at a specific absorption rate (SAR) value of 4 W/kg for 48 h. CCK-8 assays were used to determine the cell viability after 24, 48, and 72 h of irradiation. Neurite outgrowth of primary hippocampal neurons (DIV 2) and Neuro2a cells was observed with a 20 × optical microscope and recognized by ImageJ software. Rap1a and Rap1b gene expressions were detected by real-time quantitative PCR. Rap1, Rap1a, Rap1b, Rap1GAP, and p-MEK1/2 protein expressions were detected by western blot. Rap1-GTP expression was detected by immunoprecipitation. The role of Rap1-GTP was assessed by transfecting a constitutively active mutant plasmid (Rap1-Gly_Val-GFP) into Neuro2a cells.Results: Exposure to 1,800 MHz RF-EMR for 24, 48, and 72 h at 4 W/kg did not influence cell viability. The neurite length, primary and secondary neurite numbers, and branch points of primary mouse hippocampal neurons were significantly impaired by 48-h RF-EMR exposure. The neurite-bearing cell percentage and neurite length of Neuro2a cells were also inhibited by 48-h RF-EMR exposure. Rap1 activity was inhibited by 48-h RF-EMR with no detectable alteration in either gene or protein expression of Rap1. The protein expression of Rap1GAP increased after 48-h RF-EMR exposure, while the expression of p-MEK1/2 protein decreased. Overexpression of constitutively active Rap1 reversed the decrease in Rap1-GTP and the neurite outgrowth impairment in Neuro2a cells induced by 1,800 MHz RF-EMR exposure for 48 h.Conclusion: Rap1 activity and related signaling pathways are involved in the disturbance of neurite outgrowth induced by 48-h 1,800 MHz RF-EMR exposure. The effects of RF-EMR exposure on neuronal development in infants and children deserve greater focus.
Prognostic Enigma of Pancreatic Solid Pseudopapillary Neoplasm: A Single-Center Experience of 63 Patients
Authors: Wang Xinbo , Zhu Daojun , Bao Wei , Li Min , Wang Sizhen , Shen Rongxi 
Published: November 2021

Background: Studies investigating prognostic factors of solid pseudopapillary neoplasm (SPN) have been published with conflicting findings.Methods: Retrospective analysis of 63 consecutive cases of SPN in our institution from January 2010 to December 2019 was carried out. The clinicopathological features, treatment practices along with survival associations were collected and analyzed.Results: Fifteen patients (23.8%) were male, and 48 (76.2%) were female, with a median age of 34.0 ± 14.5 years. The larger tumor size was correlated with the more mixed components (p = 0.000) and the higher Ki-67 index (p = 0.042). No recurrence was found in the nine patients whose tumors fulfilled the WHO criteria for malignancy due to the presence of at least perineural invasion (6.4%), angiovascular invasion (2.3%), and/or adjacent organ invasion (6.4%). Microscopic infiltrative growth was detected in 9 (14.3%) tumors, which was correlated significantly with the WHO criteria (p = 0.002), capsule invasion (p = 0.005), and pancreatic parenchyma invasion (p = 0.001), but not with disease-free survival (p = 0.13). CD99 was found to be positively expressed in 88.9% (40/45) of tumors and more likely to have depressed Ki-67 index (p = 0.016). After a median follow-up of 58 months, only two patients (3.2%) had a recurrence after their first operation outside of our hospital. No patient died due to tumor progression.Conclusions: Although survival is favorable with aggressive surgery, it is actually difficult to assess the prognostic factors of resected SPNs. Future investigations into the role of clinicopathological evaluation will unveil the prognostic enigma of pancreatic SPN after resection.
PENDEKATAN MULTI REZIM HUKUM ( MULTI DOOR SYSTEM) PADA TINDAK PIDANA PERIKANAN
Authors: Kharisma Hamzah , Syafruddin Syafruddin 
Published: November 2021

ABSTRAK Kejahatan perikanan yang terjadi di dunia, khususnya Indonesia, dewasa ini tidak lagi terbatas pada masalah administrasi saja, Banyak tindak pidana lain yang juga terjadi bersamaan terjadi dalam tindak pidana perikanan. Perbuatan pidana di atas masing-masing mempunyai konsekuensi tersendiri yang tidak sama, ada kalanya suatu tindakan pidana yang ternyata diatur dalam lebih dari satu ketentuan pidana. Tindak pidana perikanan sebagai pidana asal (predicate crime) sering kali tidak maksimal dalam penanganannya dikarenakan kewenangan penyidik perikanan sesuai Undang undang No. 45 tahun 2009 sebagimana telah diubah dan ditambah dengan Undang undang No. 31 tahun 2004 tentang Perikanan yang terbatas mengakibatkan tidak terprosesnya tindak pidana lain. Tindak pidana perikanan sebagai kejahatan ekonomi dan lingkungan yang merupakan kejahatan yang luar biasa (extraordinary crime) sehingga harus ditangani dengan cara luar biasa (extraordinary act). Hasil penelitian ini menunjukan bahwa pendekatan multi-rezim hukum (multi door approach) terhadap tindak pidana perikanan dan tindak pidana lain terkait perikanan dapat dilakukan dengan penerapan concursus realis. Kata Kunci : Tindak Pidana Perikanan, Multi-rezim hukum, dan Concursus realis. AbstractFisheries crimes that occur in the world, especially Indonesia, today are no longer limited to administrative problems. Many other crimes that occur simultaneously occur in criminal acts of fisheries. The criminal acts above each have their own unequal consequences, there are times when a criminal act turns out to be regulated in more than one criminal provision. Fisheries criminal acts as predicate crime are often not optimal in handling due to the authority of fisheries investigators in accordance with Law No. 45 of 2009 as already amended and supplemented by Law No. 31 of 2004 concerning limited fisheries resulted in the non-processing of other criminal acts. Crime of fisheries as an economic and environmental crime which is an extraordinary crime (extraordinary crime) so it must be handled in an extraordinary way (extraordinary act). The results of this study indicate that a multi-regime approach to law (multi-door approach) to criminal acts of fisheries and other criminal acts related to fisheries can be done by applying Concursus realist. Keywords: Fisheries Crime, Multi-regime law, and Concursus Realist
TELAAH KRITIS UPAYA PENEGAKAN HUKUM TERHADAP TINDAK PIDANA KEKERASAN DALAM RUMAH TANGGA
Authors: Eleanora Fransiska Novita , Putri Elfirda Ade 
Published: November 2021

ABSTRAK Maraknya tingkat kekerasan dalam rumah tangga menyebabkan penderitaan bagi korbandan karena dapat menimbulkan taruma yang berkepanjangan dan untuk memulihkannyaperlu adanya tindakan rehabilitasi dan untuk itu perlu diberikannya sanksi yang tegasbagi pelaku. Karena dengan pemberian hukuman memberikan perlindungan danpenegakan hukum terhadap hak perempuan, sesuai dengan ketentuan undang-undangNomor 23 Tahun 2004 tentang penghapusan kekerasan dalam rumah yaitu melindungiakan hak asasi perempuan. Rumusan dari masalah ini bagaimana telaah kritis upaya daripenegakan hukum terhadap tindak pidana terhadap kekerasan dalam rumah tangga.Tujuannya adalah untuk mengetahui penegakan hukum terhadap tindak pidana kekerasandalam rumah tangga, sedangkan metode yang digunakan adalah yuridis normatif dimanadidasarkan pada konsep, asas, dan teori serta pertauran perundangan dan juga literaturyang berkaitan dengan telaah kritis terhadap upaya penegakan hukum dari tindak pidanaterhadap kekerasan dalam lingkup rumah tangga, dan hasil dari pemelitian (temuan)bahwa penegakan hukum dapat dilaksanakan jika menerapkan sanksi yang berat sepertihukuman seumur hidup kepada pelaku jika akibat perbuatannya menimbulkan luka beratbagi korban, kesimpulannya bahwa efektivitas dari penegakan hukum itu sendiridipenggruhi oleh beberapa faktor, yaitu dari hukum itu sendiri, penegak hukum, saranadan juga fasilitas, masyarakat dan kebudayaan. Kata Kunci : penegakan hukum, tindak pidana, rumah tangga ABSTRACT The rampant level of domestic violence causes suffering for victims because it can causeprolonged trauma and to restore it is done by rehabilitation measures for victims as well asproviding strict sanctions for perpetrators. Because the provision of punishment providesprotection and law enforcement for women's rights, in accordance with the provisions of lawnumber 23 of 2004 concerning the elimination of violence in the home that is protecting thehuman rights of women. The formulation of this problem is a critical study of lawenforcement efforts against domestic violence. The aim is to find out the law enforcement ofdomestic violence, while the method used is normative juridical which is based on concepts,principles, theories and regulations as well as literature relating to critical review of lawenforcement efforts on violence in the home stairs, and get the results of research (findings)that law enforcement can be implemented if applying severe sanctions such as lifeimprisonment to the offender if the consequences of his actions cause serious injuries to thevictim, the conclusion that the effectiveness of law enforcement itself is influenced by several factors, namely from law itself, law enforcement, facilities and also facilities, society and culture. Keywords: law enforcement, crime, household
PENTINGNYA KONSEP 3 IN 1 IN THE LAND ACQUTITION PADA PROSES PENYERAHAN TANAH FASILITAS UMUM (FASUM).
Authors: Hafit Husni , Muliawan Jarot 
Published: November 2021

ABSTRACLand is essential for human life. On land, humans also build houses as shelter and build various other buildings for offices and so on. Land also contains various kinds of natural resources that can be utilized by humans. The implementation of house construction as a shelter is inseparable from housing development, where the house is a basic human need which has the main function as a place to live or settle, apart from that housing also functions as a place for community interaction which is always done as a useful habit. for the welfare, security and happiness of life as a social being, housing development is certainly inseparable from the previous stages as stated in the 3 In 1 The Land Aquitition Concept ABSTRAKTanah merupakan hal penting bagi kehidupan manusia. Diatas tanah pula manusia membangun rumah sebagai tempat bernaung dan membangun berbagai bangunan lainnya untuk perkantoran dan sebagainya. Tanah juga mengandung berbagai macam kekayaan alam yang dapat dimanfaatkan manusia. Pelaksanaan pembangunan rumah sebagai tempat bernaung itu tidak lepas dari pembangunan perumahan, dimana rumah merupakan kebutuhan dasar (basic needs) manusia yang berfungsi utama sebagai tempat tinggal atau menetap, terlepas dari itu perumahan juga berfungsi sebagai tempat berinteraksi masyarakat yang selalu dilakukan sebagai suatu kebiasaan yang berguna untuk kesejahteraan, keamanan dan kebahagiaan hidup sebagai mahluk sosial, pembangunan kawasan hunian rumah tinggal maupun tempat usaha tentu tidak lepas dari tahapan – tahapan yang di lalui sebelumnya seperti halnya yang tertuang dalam Konsep 3 In 1 The land Aquitition.