bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Soil Microbial Composition in Rhizospheres Between Wilt Disease-Resistant and Susceptible Melon Varieties
Authors: Qiu Lulu , Zhu Yu , Li Xinni , Qin Yuchen , Li Guifen , Ye Yunfeng , He Yi , Huang Jinyan , Yang Shangdong 
Published: February 2025

To screen out the bio-control soil microorganisms for preventing melon wilt, soil microbial compositions in rhizospheres between wilt-resistant and susceptible melon varieties were analyzed. The results showed that the soil fungal richness in rhizospheres of wilt-resistant melon varieties (MT) was significantly higher than that of wilt-susceptible melon varieties (MS). Additionally, in comparison with MS, soil bacterial compositions, such as Proteobacteria, Bacteroidota, Acidibacter, Streptomyces, etc., and the soil fungal compositions, such as Penicillium, Derxomyces, Aspergillus, and Talaromyces, enriched; also, Trichoderma, Gibellulopsis, and Pseudallescheria decreased in rhizospheres of wilt-resistant melon varieties (MT). Moreover, Mycothermus, Zopfiella, and Cladorrhinum were the unique soil-dominant fungal genera in rhizospheres of MT. All the above results suggested that the soil bacterial communities, such as Proteobacteria, Bacteroidota, Acidibacter, Streptomyces, etc., and the soil fungal communities, such as Penicillium, Derxomyces, Aspergillus, Talaromyces Mycothermus, Zopfiella, and Cladorrhinum, could be speculated as the potential soil bio-control microorganisms for preventing melon wilt.
Activity of lysostaphin against Staphylococcus aureus and Staphylococcus epidermidis, and their biofilms
Authors: Gordina E. M. , Bozhkova S. A. , Goncharuk D. A. , Tkach E. N. , Kasimova A. R. 
Published: February 2025

Objective. To conduct a comparative evaluation of lysostaphin activity against isolates of S. aureus, S. epidermidis and their biofilms, obtained from orthopedic patients. Materials and methods. The study examines the effect of lysostaphin on 120 clinical bacterial isolates (30 MSSA, 30 MRSA, 30 MSSE, and 30 MRSE) alongside four reference strains of staphylococci. The minimum inhibitory concentration of lysostaphin was determined using a serial dilution method (ranging from 0.06 to 512 mg/l). The effect on biofilms and their formation was assessed by means of the O'Toole method. Data analysis was performed using GraphPad Prism software. Results. According to the results, lysostaphin exhibited twice the activity against methicillin-sensitive strains and was more effective against S. aureus compared to S. epidermidis. The studied concentrations of lysostaphin effectively prevented biofilm formation, particularly in MSSA strains. The MBIC90 value for lysostaphin was found to be four times higher for MRSE strains and twice as high for S. aureus. Additionally, the MBEC90 value of lysostaphin against S. epidermidis was 32 times greater than that observed for S. aureus. Conclusion. The pronounced anti-staphylococcal activity of lysostaphin, along with its significant destructive effect on S. aureus biofilms, offers a considerable potential for further investigation and implementation in clinical practice to combat staphylococcal infections, including those associated with various implants in orthopedics, dentistry, and cardiology.
Valvular bronchoblockation in destructive pulmonary tuberculosis in Primorsky Krai
Authors: Tsyleva Yu. I. , Belov S. A. 
Published: February 2025

Objective. To determine the efficacy of valvular bronchial blocking in patients with destructive pulmonary tuberculosis and to examine the factors affecting the performance of the technique. Materials and methods. The study analyzes the outcomes of comprehensive treatment for 66 patients diagnosed with destructive pulmonary tuberculosis. The treatment was provided at a tuberculosis dispensary from 2021 to 2024 and involved valvular bronchial blocking. Results. One year following the intervention, the technique resulted in the closure of cavitary lesions in 56.1 % (37/66) of the patients, and the cessation of bacteriological excretion in 65.2 % (15/23). Notably, the effectiveness of cavity closure in infiltrative pulmonary tuberculosis amounted to 84.6 % (33/39), whereas in fibrocavernous tuberculosis – only 14.8 % (4/27), with a statistically significant difference (p < 0.05). In addition, the optimal efficacy was registered in cases with the duration of the disease to be less than one year (65 % success), with cavitary lesions up to 2 cm (78.6 %) or 2–4 cm (67.7 %). No correlation was observed between treatment efficacy and drug susceptibility of Mycobacterium tuberculosis. Complications of bronchial blocking developed in 16 out of 66 patients (24.3 %) and were resolved through repeat bronchoscopy. Conclusion. Valvular bronchial blocking appears to be an effective non-resectional technique for the treatment of patients with destructive pulmonary tuberculosis and requires personalized criteria for intervention.
Laser refractive surgery in ophthalmology
Authors: Melnikov V. Y. , Shcherbakov I. A. , Filina N. V. , Polishchuk A. S. , Chichilimov A. V. 
Published: February 2025

Objective. To evaluate the effiacy of surgical treatment for myopia through laser refractive surgeries utilizing an innovative technology – Refractive Lenticule Extraction/ Small-incision Lenticule Extraction (ReLEx SMILE). Materials and methods. The study involved 92 eyes with myopia (spherical equivalent (S.E.) ranging from −2.0 to −10.0 diopters and astigmatism not exceeding −3.0 diopters). The main group consisted of 21 patients (42 eyes) who underwent bilateral surgical treatment using ReLEx SMILE via femtosecond lamellar extraction through a small incision. In the control group, 25 patients (50 eyes) underwent bilateral standard Laser-Assisted in Situ Keratomileusis (LASIK) surgery. Results. One month after surgery, the S.E. in the ReLEx SMILE group accounted for −0.1 ± 0.2 diopters, −0.13 ± 0.34 diopters at three months, and −0.13 ± 0.40 diopters at six months. In the control group, S.E. comprised 0.15 ± 0.31 diopters at one month, −0.09 ± 0.27 diopters at three months, and −0.18 ± 0.43 diopters at six months of observation. Six months after surgery, 36 % (15 eyes) in the ReLEx SMILE group gained one line on the visual acuity chart; 48 % (20 eyes) remained unchanged, while 14 % (6 eyes) lost one line, and one eye lost two lines. In the LASIK control group, 28 % (14 eyes) gained one line, 62 % (31 eyes) remained unchanged, and 10 % (5 eyes) lost one line. No patients lost two lines. In the ReLEx SMILE group, all 42 eyes achieved a visual acuity of 0.7–0.9 or better six months after surgery, with 66 % (28 eyes) achieving 1.0–1.2. In the LASIK group, all 50 eyes had a visual acuity of 0.7–0.9 or better, with 75 % (38 eyes) achieving 0.9–1.0 or better. Conclusion. The ReLEx SMILE method for refractive correction of moderate to high myopia demonstrates comparable efficacy, safety, and predictability to LASIK surgery, while exhibiting superior stability.
Antiretroviral resistance testing results in patients with HIV living in the Primorye Krai
Authors: Markelova E. V. , Beniova S. N. , Knysh S. V. , Gorelova I. S. , Ogneva Z. V. , Eliseeva V. S. , Chagina E. A. 
Published: February 2025

Objective. To describe the drug resistance and genetic diversity of HIV-1 among patients in Primorsky Krai in 2024. Materials and methods. A biological material analysis was conducted on 122 HIV-infected patients under dispensary observation at the at the AIDS Center of Regional Hospital No. 2 (Vladivostok, Russia) in order to identify the drug resistance to four main classes of antiretroviral agents. Results. A high prevalence of genetic variants A6 (44.3 %), B (28.7 %), and C (8.2 %) of HIV-1 was observed among the examined patients. The likelihood of non-nucleoside reverse transcriptase inhibitor resistance was found to be tenfold higher in subtype B compared to subtype A6 (OR = 10.5). The integrase inhibitor resistance was significantly more frequently detected in the subgroup of patients with HIV-1 subtype B (OR = 38.1). In the overall patient sample, the drug resistance was identified in 19.7 % of cases for one group of antiretroviral medications, in 9 % for two groups, and in 2.5 % for three groups of medications. A recombinant form A6/CRF02_AG was detected in 8.2 % of cases. Additionally, 10.6 % of cases exhibited various genetic variants of HIV-1 which can be classified as new recombinant or unique forms. Conclusion. The results demonstrate a rising trend in recombinant types of HIV-1 within the region, reflecting changes in the overall genetic landscape of HIV subtypes, with significant variations in the mutation patterns associated with drug resistance across certain groups of antiretroviral therapy in both B and non-B subtypes of HIV.
Alternative interval scale for assessing erectile function
Authors: Danilov V. V. , Vashchenko V. V. , Danilov V. V. , Radko D. А. , Danilov V. V. 
Published: February 2025

Aim. To develop an alternative system for assessing erectile function based on an interval scale. Materials and methods. Materials were collected using the EMBASE, MEDLINE, Cochrane Central (Cochrane HTA, DARE, HEED), Google Scholar and the WHO International Clinical Trials Platform search databases. The search query included the following key phrases: Erectile dysfunction; IIEF-15, IIEF-5, system for assessing clinical symptoms in medicine. Results. To develop an alternative system for assessing erectile function, a table based on an interval scale was created. The approach used in the diagnostic scoring system was used, according to which a score is assigned according to the threatometric evaluation of each symptom gradation in the form of a single-digit number reflecting the ratio of probabilities of differentiated states A2 and A1 and representing the decimal logarithm of this ratio multiplied by 5 and rounded to an exact value. Conclusion. The study presents an alternative system for assessing erectile function based on an interval scale that allows quantifying the severity of erectile dysfunction and its individual symptoms. According to the concept of interval scales, the table allows arithmetic operations with variables (symptoms evaluated in points) to be performed, thus representing a tool for managing patients with erectile dysfunction and its correction by modern pharmacological agents.
Decreased herd immunity, measles and pertussis outbreaks in 2023
Authors: Sakhabetdinov B. A. , Sakhabetdinova K. N. , Ibragimova A. R. , Shpaner V. I. , Safiullina N. D. 
Published: February 2025

Objective. To analyze vaccination coverage and identify the characteristics of measles and pertussis morbidity among children in 2023. Materials and methods. The study involved an analysis of outpatient records from the City Children's Polyclinic No. 6 in Kazan. A total of 305 children aged 1 to 18 years (mean age 10.2 ± 0.33 years) were examined during the first quarter of 2023. The distribution by sex was as follows: boys – 54.75 % (n = 167), girls – 45.25 % (n = 138). Results. The analysis of outpatient records, vaccination certificates, and patient examinations revealed that 228 children (74.75 % of those examined) had received complete vaccinations, 41 children (13.45 %) had received partial vaccinations, and 36 children (11.8 %) had not been vaccinated at all with the adsorbed pertussis-diphtheria-tetanus vaccine. The analysis of measles vaccination coverage indicated that 74 children (48.68 % of those examined) had received complete vaccinations, 58 children (38.1 %) had received partial vaccinations, and 20 children (13.5 %) had not been vaccinated against measles. Conclusion. Reducing herd immunity to 74.75 % determines the necessity for vaccination initiatives aimed at increasing herd immunity to the target level of 95 %.
Algorithm for Analyzing Computer Images in Radiation Diagnostics of Changes in the Cervical-Cranial Region in Dysfunction of the Temporomandibular Joints
Authors: Samuilau I. U. , Baradina I. N. , Vasilyeva Yu. F. , Neden V. Ch. 
Published: February 2025

To improve the results of diagnostics of anatomical structures in dentistry, a method such as cone-beam computed tomography is used, which provides extended visualization of the anatomical structures of the dental system and adjacent areas, including the cervical region, upper respiratory tract, etc. To improve the results of diagnostics of patients with diseases of the temporomandibular joints, an algorithm for analyzing a computer image of the cervicocranial region is proposed, which has diagnostic reproducibility, allows determining the asymmetry of the mutual arrangement of anatomical structures, detecting degenerative-dystrophic and other changes in the articulating surfaces. This method helps to identify the etiological factors in the development of diseases of the temporomandibular joints, increases the effectiveness of treating other diseases of the cervicocranial region.
The influence of biologization of fertilizer systems on the content and stock of humus in a of typical chernozem light loamy
Authors: Kucher L. , Vitvitskyi S. , Kava L. , Kucher T. , Kravchenko Yu. 
Published: February 2025

Goal. To investigate the humus state of typical light loamy chernozem and the yield of winter wheat under the conditions of soil protection technologies.
Methods. The following methods were used: informative and analytical method for collecting materials from literature sources; field research — observation of plants, accounting and harvesting; laboratory, mathematical and statistical methods — processing of results. The research was conducted on typical coarse-dusty light loam black soil in the forest, the crop was winter wheat, Poliska 90 variety. The comparative effectiveness of the technologies was studied: traditional, based on shelf plowing to a depth of 25—27 cm; soil protection, based on shallow flat-cutting to a depth of 10—12 cm. The effect of fertilization systems was studied against the background of these cultivation technologies: 1 — control (no fertilizers); 2 — straw 1.2 t/ha + N12 + N55P45K45; 3 — straw 1.2 t/ha + N12 + N78P68K68; 4 — straw 1.2 t/ha + N12 + green manure + N55P45K45; 5 — straw 1.2 t/ha + N12 + green manure + N78P68K68.
Results. The use of soil protection technology compared to the traditional one did not significantly change the humus content in the arable layer, but affected its redistribution. The highest humus content (3.77%) was recorded in the variant straw 1.2 t/ha + N12+ green manure + N78P68K68 against the background of soil protection technology in the upper soil layer under study. Organic fertilizers increased the content of mobile organic matter in the 0—30 cm layer against the background of plowing by 0.063% relative, and against the background of soil protection technology — by 0.176%. When straw was applied with mineral fertilizers, the content of humic acids in chernozem was typically 0.042—0.052% under conventional technology and 0.055—0.088% under soil protection technology.
Conclusions. In the variant with the introduction of straw 1.2 t/ha + N12 + green manure + N78P68K68, the content of humic acids was 1.5 times higher than in the same variant with the traditional technology. Soil protection technology led to an increase in the yield of winter wheat by 3.5 c/ha compared to the traditional technology.
Efficiency of systems of biological protection of potatoes against diseases
Authors: Solomiychuk M. , Pikovskyi M. 
Published: February 2025

Goal. To investigate the application of various potato protection schemes based on biological preparations and to determine their effectiveness against the most harmful diseases.
Methods. Laboratory (pathogen identification, disease diagnosis) and field (vegetation indicators analysis) research methods. The study of the effectiveness of biological preparations was carried out on the basis of the Ukrainian Research Plant Quarantine Station of the Institute of Plant Protection against a natural infectious background. During the growing season, three foliar treatments with drugs were carried out.
Results. The use of biological preparations of a fungicidal and stimulating nature contributed to the growth of yield and improvement of the marketability of potatoes. The highest yield was formed by treatment with the preparation BioHybervit BT and amounted to 21.3 t/ha. The best protection of potatoes against diseases was obtained with the use of Bactophyt BT, where the effectiveness against Alternaria was 66.8%, and against late blight — 89.8%. Stimulating drugs provided an immunoprotective effect of 65—70% against phytophthora and 40—47% against Alternaria. The best indicators of tuber formation and productivity were noted in scheme 4, which included the use of the following drugs: Vitastim BT, Trihopsyn BT, Gliocladin BT, Biospectr BT, Bactophyt BT and chelates 1 and 2, where the weight of tubers per plant was 693 g/plant.
Conclusions. The use of a number of biological preparations during the growing season contributed to the control of the main potato diseases and the preservation of the crop. Biological drugs and growth stimulants that showed the highest efficiency were used for the development of potato protection schemes. The use of a system of biopreparations, which included 4 consecutive treatments with combinations of the stimulating nature drug Vitastim BT with BT fungicides and chelated microelements, provided the best protection. Against phytophthora this indicator was 78.4% and 78.9% against Alternaria.
PENDAMPINGAN PENGEMBANGAN LKPD BERBASIS COMPUTATIONAL THINKING UNTUK PEMBELAJARAN BERDIFERENSIASI BAGI GURU-GURU MGMP SMA KABUPATEN MUSI BANYUASIN
Authors: Hapizah Hapizah , Mulyono Budi , Aisyah Nyimas , Pratiwi Weni Dwi , Sukma Yovika , Ramadhan M. Hasbi 
Published: February 2025

Kegiatan mendampingi pengembangan Lembar Kerja Peserta Didik (LKPD) berbasis computational thinking dalam pembelajaran berdiferensiasi adalah bentuk pengabdian kepada masyarakat, dengan tujuan meningkatkan kualitas profesionalisme guru matematika di tingkat SMA Kabupaten Musi Banyuasin. Proses kegiatan pengabdian ini mencakup persiapan, pendampingan, implementasi, dan tindak lanjut. Kegiatan dilakukan secara langsung di SMP Negeri 6 Sekayu, diikuti oleh diskusi melalui grup Whatsapp, dan akhirnya melalui pertemuan online menggunakan Zoom. Sebagai bagian dari evaluasi, kuesioner disebarluaskan, dan hasilnya menunjukkan bahwa guru-guru memberikan penilaian rata-rata sebesar 88,65%, menunjukkan kepuasan yang tinggi terhadap kegiatan pengabdian ini. Hasil pendampingan menyimpulkan bahwa secara keseluruhan, guru-guru matematika di SMA Kabupaten Musi Banyuasin telah berhasil menyusun LKPD berbasis computational thinking untuk pembelajaran berdiferensiasi, meskipun masih memerlukan pelatihan dan peningkatan lebih lanjut.
Kata kunci: lembar kerja peserta didik, computational thinking, pembelajaran berdiferensiasi
PENDAMPINGAN PENGGUNAAN LEMBAR KERJA DIGITAL INTERAKTIF UNTUK GURU IPA KABUPATEN MUARA ENIM
Authors: Sari Diah Kartika , Ad’hiya Eka , Haryani Maefa Eka , Gulo Fakhili 
Published: February 2025

Pesatnya perkembangan teknologi pada abad ini tentunya membutuhkan sumber daya manusia yang kompeten agar dapat beradaptasi sekaligus berkontribusi terhadap perkembangan ilmu pengetahuan dan teknologi tersebut, yang mampu meningkatkan keterampilan abad 21, salah satunya literasi digital. Salah satu cara menggunakan teknologi dalam pembelajaran yaitu dengan melalui penggunaan Lembar Kerja Digital yang interaktif. Oleh karena itu diperlukan pendampingan penggunaan lembar kerja digital interaktif untuk melatih literasi digital guru, dalam hal ini yang menjadi khalayak sasaran adalah guru IPA kabupaten Muara Enim. Kegiatan pengabdian ini terdiri dari tiga tahapan yaitu penyampaian materi, praktik dan evaluasi. Khalayak sasaran pada pengabdian ini yaitu guru IPA di Kabupaten Muara Enim. Dari kegiatan pengabdian dapat disimpulkan bahwa khalayak sasaran pada mulanya belum menggunakan media dan evaluasi yang berbasis digital. Dengan diperkenalkan dan dipraktikkan penggunaan digital dalam pembelajaran, khalayak sasaran menjadi berminat dan tertarik karena lebih variatif, bisa menggunakan video, audio, animasi, dan simulasi. Media digital ini mudah digunakan oleh guru dan dapat membuat pembelajaran menjadi lebih menyenangkan.

AbstractThe rapid development of technology in this century certainly requires competent human resources to adapt and contribute to the development of science and technology, which can improve 21st-century skills, one of which is digital literacy. One way to use technology in learning is through interactive Digital Worksheets. Therefore, interactive digital worksheet assistance is needed to train digital literacy teachers; in this case, the target audience is the Muara Enim Regency science teachers. This community service activity consists of three stages: delivering material, practice and evaluation. The target audience for this community service is science teachers in Muara Enim Regency. From the community service activities, the target audience initially did not use digital-based media and evaluation. By introducing and practising the use of digital in learning, the target audience becomes interested and attracted because it is more varied and can use video, audio, animation, and simulation. This digital media is accessible to teachers and can make learning more enjoyable.
EFFECT OF FAMILY-CENTERED CARE IMPLEMENTATION ON REDUCING PARENTAL STRESS IN THE GARDENIA ROOM AT MUHAMMAD SANI HOSPITAL, 2024
Authors: Virona Lola , Putri Angga , Dewi Silalahi Ratna 
Published: February 2025

Hospitalizing a child can be a very stressful experience for parents and can cause stress. From the results of interviews with 5 parents, it was found that most experienced symptoms of stress such as changes in diet, sleep and fatigue, while observation results showed various behaviors such as daydreaming, using smartphones when looking after children, and speaking in a commanding tone to nurses. This research was conducted to determine the effect of implementation family centered care on hospitalization stress in parents with children being treated in the Gardenia room at Muhammad Sani Regional Hospital in 2024. This research was conducted in July 2024 with 42 parents as respondents. The experimental research method that will be used is research design Pre-Experimental Design. Average parental stress level before intervention Family Centered Care (FCC) in the Gardenia room at Muhammad Sani Regional Hospital in 2024 was 18.54 and after intervention it was 12.61. Effect test results using Wilcoxon Signed Ranks Test obtained a significance value of 0.000 <0.05 so it can be concluded that there is an influence of implementation family centered care on hospitalization stress in parents with children being treated in the Gardenia room at Muhammad Sani Regional Hospital in 2024.