bluuebunny/crossref_metadata_2025_split huggingface dataset:
A β‐lactamase gene of Fusarium oxysporum alters the rhizosphere microbiota of soybean
Authors: Chang Hao‐Xun , Noel Zachary A. , Chilvers Martin I. 
Published: March 2021

SummaryThe rhizosphere is a multitrophic environment, and for soilborne pathogens such as Fusarium oxysporum, microbial competition in the rhizosphere is inevitable before reaching and infecting roots. This study established a tritrophic interaction among the plant growth‐promoting rhizobacterium Burkholderia ambifaria, F. oxysporum and Glycine max (soybean) to study the effects of F. oxysporum genes on shaping the soybean microbiota. Although B. ambifaria inhibited mycelial growth and increased bacterial propagation in the presence of F. oxysporum, F. oxysporum still managed to infect soybean in the presence of B. ambifaria. RNA‐Seq identified a putative F. oxysporum secretory β‐lactamase‐coding gene, FOXG_18438 (abbreviated as Fo18438), that is upregulated during soybean infection in the presence of B. ambifaria. The ∆Fo18438 mutants displayed reduced mycelial growth towards B. ambifaria, and the complementation of full Fo18438 and the Fo18438 β‐lactamase domain restored mycelial growth. Using the F. oxysporum wild type, ∆Fo18438 mutants and complemented strains with full Fo18438, Fo18438 β‐lactamase domain or Fo18438 RTA1‐like domain for soil inoculation, 16S rRNA amplicon sequencing revealed that the abundance of a Burkholderia operational taxonomic unit (OTU) was increased in the rhizosphere microbiota infested by the strains with Fo18438 β‐lactamase domain. Non‐metric multidimensional scaling and PICRUSt2 functional analysis revealed differential abundance for the bacterial β‐lactam‐related functions when contrasting the genotypes of F. oxysporum. These results indicated that the Fo18438 β‐lactamase domain provides F. oxysporum with the advantage of growing into the soybean rhizosphere, where β‐lactam antibiosis is involved in microbial competition. Accordingly, this study highlights the capability of an F. oxysporum gene for altering the soybean rhizosphere and taproot microbiota.
Utility of venous blood gases for the assessment of traumatic shock: a prospective observational study
Authors: Coggins Andrew R , Vivekanandamoorthy Nurojan , Byth Karen , Aleemullah Tabish , Selvendran Selwyn T , Watkins Rachel J , Shetty Amith L , Devjak Lorraine , Hsu Jeremy M 
Published: March 2021

BackgroundABG samples are often obtained in trauma patients to assess shock severity. Venous blood gas (VBG) sampling, which is less invasive, has been widely used to assess other forms of shock. The study aim was to determine the agreement between VBG and ABG measurements in trauma.MethodsPatients were enrolled at an Australian trauma centre between October 2016 and October 2018. Bland-Altman limits of agreement (LOA) between paired blood gas samples taken <30 min apart were used to quantify the extent of agreement. The impact of using only VBG measurements was considered using an a priori plan. Cases where venous sampling failed to detect ‘concerning levels’ were flagged using evidence-based cut-offs: pH ≤7.2, base deficit (BD) ≤−6, bicarbonate <21 and lactate ≥4. Case summaries of these patients were assessed by independent trauma clinicians as to whether an ABG would change expected management.ResultsDuring the study period 176 major trauma patients had valid paired blood gas samples available for analysis. The median time difference between paired measurements was 11 min (IQR 6–17). There was a predominance of men (81.8%) and blunt trauma (92.0%). Median Injury Severity Score was 13 (range 1–75) and inpatient mortality was 6.3%. Mean difference (ABG−VBG) and LOA between paired arterial and venous measurements were 0.036 (LOA −0.048 to 0.120) for pH, −1.27 mmol/L (LOA −4.35 to 1.81) for BD, −0.64 mmol/L (LOA −1.86 to 0.57) for lactate and −1.97 mmol/L (LOA −5.49 to 1.55) for bicarbonate. Independent assessment of the VBG ‘false negative’ cases (n=20) suggested an ABG would change circulatory management in two cases.ConclusionsIn trauma patients VBG and ABG parameters displayed suboptimal agreement. However, in cases flagged as VBG ‘false negative’ independent review indicated that the availability of an ABG was unlikely to change management.
Pan-ERBB kinase inhibition augments CDK4/6 inhibitor efficacy in oesophageal squamous cell carcinoma
Authors: Zhou Jin , Wu Zhong , Zhang Zhouwei , Goss Louisa , McFarland James , Nagaraja Ankur , Xie Yingtian , Gu Shengqing , Peng Ke , Zeng Yong , Zhang Xiaoyang , Long Henry , Nakagawa Hiroshi , Rustgi Anil , Diehl J Alan , Meyerson Matthew , Wong Kwok-Kin , Bass Adam 
Published: March 2021

ObjectiveOesophageal squamous cell carcinoma (OSCC), like other squamous carcinomas, harbour highly recurrent cell cycle pathway alterations, especially hyperactivation of the CCND1/CDK4/6 axis, raising the potential for use of existing CDK4/6 inhibitors in these cancers. Although CDK4/6 inhibition has shown striking success when combined with endocrine therapy in oestrogen receptor positive breast cancer, CDK4/6 inhibitor palbociclib monotherapy has not revealed evidence of efficacy to date in OSCC clinical studies. Herein, we sought to elucidate the identification of key dependencies in OSCC as a foundation for the selection of targets whose blockade could be combined with CDK4/6 inhibition.DesignWe combined large-scale genomic dependency and pharmaceutical screening datasets with preclinical cell line models, to identified potential combination therapies in squamous cell cancer.ResultsWe identified sensitivity to inhibitors to the ERBB family of receptor kinases, results clearly extending beyond the previously described minority of tumours with EGFR amplification/dependence, specifically finding a subset of OSCCs with dual dependence on ERBB3 and ERBB2. Subsequently. we demonstrated marked efficacy of combined pan-ERBB and CDK4/6 inhibition in vitro and in vivo. Furthermore, we demonstrated that squamous lineage transcription factor KLF5 facilitated activation of ERBBs in OSCC.ConclusionThese results provide clear rationale for development of combined ERBB and CDK4/6 inhibition in these cancers and raises the potential for KLF5 expression as a candidate biomarker to guide the use of these agents. These data suggested that by combining existing Food and Drug Administration (FDA)-approved agents, we have the capacity to improve therapy for OSCC and other squamous cancer.
Liver involvement in patients with coeliac disease: proof of causality using IgA/anti-TG2 colocalisation techniques
Authors: Dutta Rimlee , Iqbal Asif , Das Prasenjit , Palanichamy Jayanth Kumar , Singh Alka , Mehtab Wajiha , Chauhan Ashish , Aggarwal Ashish , Sreenivas Vishnubhatla , Ahuja Vineet , Datta Gupta Siddhartha , Makharia Govind K 
Published: March 2021

AimsDespite clinical evidence of liver involvement in patients with coeliac disease (CeD), there is a lack of a method to prove this association.MethodsOf 146 treatment-naive patients with CeD, 26 had liver dysfunction. Liver biopsies and corresponding small intestinal biopsies were obtained from these 26 patients. Multicolour immunohistochemical and immunofluorescence confocal microscopic studies were performed on paraffin-embedded tissue to detect the IgA/anti-TG2 deposits. Follow-up liver biopsies were taken after a gluten-free diet.ResultsTwenty-six out of the 146 patients (17.8%) with suspected coeliac-associated liver disease on histological examination revealed irregular sinusoidal dilatation in 15 (57.6%), steatohepatitis in 4 (15.3%), non-specific chronic hepatitis in 3 (11.5%), autoimmune hepatitis in 2 (7.6%) biopsies, including cirrhosis in one of them, irregular perisinusoidal fibrosis and changes of non-cirrhotic portal fibrosis in one biopsy each (3.8%). IgA/anti-tTG deposits were observed in 22 (84.6%) liver biopsies by dual immunohistochemistry technique, and in 24 (92.3%) by confocal immunofluorescence technique and in all corresponding duodenal biopsies (100%). Overall, IgA/anti-tTG deposits showed 100% sensitivity, 77% specificity and 85% positive predictive value for establishing an association of extraintestinal pathology and CeD using archived tissues. Follow-up liver biopsies could be obtained in five patients; four of them showed not only resolution of the histological lesions but disappearance of IgA/anti-tTG co-localisation.ConclusionsData of the present study adds to the body of evidence that liver lesions in patients with CeD are disease related and may have been caused by a similar pathogenic mechanism that causes intestinal changes.
Factors associated with neck pain in fighter aircrew: a systematic review and meta-analysis
Authors: Wallace James B , Newman Phil M , McGarvey Aoife , Osmotherly Peter G , Spratford Wayne , Gabbett Tim J 
Published: March 2021

Neck pain is a common complaint among fighter aircrew, impacting workforce health and operational capability. This systematic review aimed to identify, evaluate and synthesise the current evidence for factors associated with the occurrence of neck pain among fighter aircrew. Six electronic databases were searched in June 2019 and updated in June 2020 utilising the maximum date ranges. Included studies were appraised for methodological quality, ranked according to level of evidence and relevant data extracted. Where methods were homogeneous and data availability allowed, meta-analyses were performed. A total of 20 studies (16 cross sectional, one case–control, one retrospective cohort and two prospective cohort) were eligible for inclusion. Of the 44 factors investigated, consistent evidence was reported for greater occurrence of neck pain among aircrew operating more advanced aircraft and those exposed to more desk/computer work, while another 12 factors reported consistent evidence for no association. Of the 20 factors where meta-analyses could be performed, greater occurrence of neck pain was indicated for aircrew: flying more advanced aircraft, undertaking warm-up stretching and not placing their head against the seat under greater +Gz. Despite many studies investigating factors associated with neck pain among fighter aircrew, methodological limitations limited the ability to identify those factors that are most important to future preventive programmes. High-quality prospective studies with consistent use of definitions are required before we can implement efficient and effective programmes to reduce the prevalence and impact of neck pain in fighter aircrew. PROSPERO registration number: CRD42019128952.Neck pain is a common complaint among fighter aircrew, impacting workforce health and operational capability. This systematic review aimed to identify, evaluate and synthesise the current evidence for factors associated with the occurrence of neck pain among fighter aircrew.
A Refined Structure Preserving Image Abstraction Framework as a Pre-Processing Technique for Desire Focusing on Prominent Structure and Artistic Stylization
Authors: Pavan Kumar M. P. , Poornima B. , Nagendraswamy H. S. , Manjunath C. , Rangaswamy B. E. 
Published: March 2021

This work identifies the strong dominant features by its location and extracts the image features for the purpose of automatic desire focusing on prominent structure and artistic stylization of images. At the pre-processing level, dataset image is treated using refined structure preserving image abstraction framework which can deliver the best effectual structure preserved abstracted results by utilizing visual attributes from 2D color image. The presented framework efficiently conserves the structural characteristics in the foreground of an input image by exhaustively amalgamate the series of non-photorealistic rendering image filters over meticulous investigational work and it also reduces the background substance of an image. The framework assesses image and object space details to generate structure preserved image abstraction thus distinguishing the accentuated elements of an enhanced structures using Harris key-point feature detector and chooses the 100 major unique dominant feature locations among available features. This work automatically selects the unique location from the extracted features using polynomial region of interest and unselected image regions and its background are blurred using Gaussian motion blurring with point spread function. Deblurring the selected region using wiener filtering to get the desire focusing on prominent structure followed by color quantization and flow-based bilateral filtering is applied over focused structural region to achieve artistic stylization. Efficiency of the framework has been validated by carrying out the trials on the selected Flickr repository, David Mould and Ruixing Wang dataset. In addition, user’s visual opinion and the image quality estimation methods were also utilized to appraise the proposed pre-processing framework. This work lists the structure preserving image abstraction framework applications, limitation, execution difficulties and future work in the field of Non-photorealistic rendering domain.
Embolic Stroke of Undetermined Source and Patent Foramen Ovale
Authors: Strambo Davide , Sirimarco Gaia , Nannoni Stefania , Perlepe Kalliopi , Ntaios George , Vemmos Kostantinos , Michel Patrik 
Published: March 2021

Background and Purpose:
The Risk of Paradoxical Embolism (RoPE) score stratifies patients with stroke according to the probability of having a patent foramen ovale (PFO), which (through Bayes theorem and simple assumptions) can be used to estimate the probability that a PFO is pathogenic in a given subgroup of patients with specific features (ie, a given RoPE score value): a higher PFO prevalence corresponds to a higher probability that a PFO is pathogenic. Among alternative mechanisms in embolic stroke of undetermined source (ESUS), the actual stroke cause may be covert atrial fibrillation. We aimed to validate the RoPE score in a large ESUS population and investigate the rate of stroke recurrence and new incident atrial fibrillation during follow-up according to PFO status and RoPE score.

Methods:
We pooled data of consecutive patients with ESUS from 3 prospective stroke registries. We assessed RoPE score’s calibration and discrimination for the presence of PFO (and consequently for the probability that it is pathogenic). Multivariate logistic regression analysis was performed to identify factors associated with PFO.

Results:

Among 455 patients with ESUS (median age 59 years), 184 (40%) had PFO. The RoPE score’s area under the receiver operating characteristic curve was 0.75. In addition to RoPE score variables, absence of left ventricular hypertrophy, absence of atherosclerosis, and infratentorial lesions were independently associated with PFO. In patients with PFO and RoPE 7 to 10, PFO and RoPE 0 to 6, and without PFO, new incident atrial fibrillation rate was 3.1%, 20.5%, and 31.8%, respectively (log-rank test=6.28,
P
=0.04). Stroke recurrences in patients with likely pathogenic PFO were not statistically different from other patients.

Conclusions:
This multicenter study validates the RoPE score to predict the presence/absence of PFO in patients with ESUS, which strongly suggests that RoPE score is helpful in identifying patients with ESUS with pathogenic versus incidental PFOs. Left ventricular hypertrophy, atherosclerosis, and infratentorial stroke may further improve the score. Low RoPE scores were associated with more incidental atrial fibrillation during 10-year follow-up.
Drop when the stakes are high: adaptive, flexible use of dropping behaviour by aphids
Authors: Humphreys Rosalind K. , Ruxton Graeme D. , Karley Alison J. 
Published: March 2021

Abstract
For herbivorous insects, dropping from the host plant is a commonly-observed antipredator defence. The use of dropping compared to other behaviours and its timing in relation to contact with a predator was explored in both pea aphids (Acyrthosiphon pisum) and potato aphids (Macrosiphum euphorbiae). Pea aphids dropped more frequently in response to ladybird adults (Adalia bipunctata) than lacewing larvae (Chrysoperla carnea). Potato aphids mainly walked away or backed-up in response to both predator types; but they dropped more frequently relative to other non-walking defences when faced with ladybird adults. Contact with a predator was an important influencer of dropping for both species, and most drops occurred from adjacent to the predator. Dropping appears to be a defence adaptively deployed only when the risk of imminent predation is high; factors that increase dropping likelihood include presence of faster-foraging predators such as adult ladybirds, predator proximity, and contact between aphid and predator.
Living in Utopia in the 19th Century
Authors: Lallement Michel 
Published: March 2021

Abstract
Concrete utopias have received little international comparison. In order to contribute to a comparative sociology of such social experiments, this article is interested in the case of France and the United States in the 19th century. To mirror concerns that were important at that time in both of these countries (the “social question” and the “question of women”), attention is focused on local experiments where work and gender were the subject of some notable innovations. After highlighting the form, importance and dynamics of abstract and concrete utopias in France and the United States, two communities inspired by C. Fourier are compared: the Familistère de Guise (France), and the Oneida Association (United States). If both learn about the Fourierist utopia, they put it into practice differently, in particular because of issues specific to each of the two countries.
Policy Analysis of Educational Services for the One Belt One Road Initiative under the covid-19 Epidemic
Authors: Tingzhou Li , Sha Lu , Yuyang Zhao , Wanying Li 
Published: March 2021

Abstract
Education undertakes the era mission of serving China’s external development strategy, and provides comprehensive personnel support, constructive intellectual support, shared humanistic exchanges, and harmonios understanding of value for the interconnections among countries along the One Belt One Road (obor). Since 2014, China’s policies on “education + ‘One Belt One Road’” have been continuously refined and expanded, and a policy system of education’s services for the obor initiative has been initially formed, which providing high-quality and top-level designs for promoting the construction of obor initiative. Educational services for the obor initiative are facing new challenges and opportunities under the global covid-19 epidemic. For example, the international misunderstandings and stigmatizations bring opportunities for introspecting the construction of China’s international discourse system; the traditional cooperation modes of offline education is blocked, which brings opportunities for exploring new forms of educational services; the contents and modes of education are becoming increasingly diversified, putting forward higher requirements for the comprehensive qualities of each educational subject. In the future period of normalization of epidemic prevention and control, it is urgent to improve the quality of educational services by maintaining strategical focuses, such as deepening the educational opening up and cooperation, optimizing the personnel training concepts and teaching modes, and improving the guarantee mechanism.
FoxO1-GAB1 axis regulates homing capacity and tonic AKT activity in chronic lymphocytic leukemia
Authors: Seda Vaclav , Vojackova Eva , Ondrisova Laura , Kostalova Lenka , Sharma Sonali , Loja Tomas , Mladonicka Pavlasova Gabriela , Zicha Daniel , Kudlickova Peskova Marie , Krivanek Jan , Liskova Kvetoslava , Kren Leos , Benes Vladimir , Musilova Litzmanova Katerina , Borsky Marek , Oppelt Jan , Verner Jan , Pospisilova Sarka , Brychtova Yvona , Panovska Anna , Tan Zhi , Zhang Shuxing , Doubek Michael , Amruz Cerna Katerina , Mayer Jiri , Mraz Marek 
Published: March 2021

Abstract
Recirculation of chronic lymphocytic leukemia (CLL) cells between the peripheral blood and lymphoid niches plays a critical role in disease pathophysiology, and inhibiting this process is one of the major mechanisms of action for B-cell receptor (BCR) inhibitors such as ibrutinib and idelalisib. Migration is a complex process guided by chemokine receptors and integrins. However, it remains largely unknown how CLL cells integrate multiple migratory signals while balancing survival in the peripheral blood and the decision to return to immune niches. Our study provided evidence that CXCR4/CD5 intraclonal subpopulations can be used to study the regulation of migration of CLL cells. We performed RNA profiling of CXCR4dimCD5bright vs CXCR4brightCD5dim CLL cells and identified differential expression of dozens of molecules with a putative function in cell migration. GRB2–associated binding protein 1 (GAB1) positively regulated CLL cell homing capacity of CXCR4brightCD5dim cells. Gradual GAB1 accumulation in CLL cells outside immune niches was mediated by FoxO1-induced transcriptional GAB1 activation. Upregulation of GAB1 also played an important role in maintaining basal phosphatidylinositol 3-kinase (PI3K) activity and the “tonic” AKT phosphorylation required to sustain the survival of resting CLL B cells. This finding is important during ibrutinib therapy, because CLL cells induce the FoxO1-GAB1-pAKT axis, which represents an adaptation mechanism to the inability to home to immune niches. We have demonstrated that GAB1 can be targeted therapeutically by novel GAB1 inhibitors, alone or in combination with BTK inhibition. GAB1 inhibitors induce CLL cell apoptosis, impair cell migration, inhibit tonic or BCR-induced AKT phosphorylation, and block compensatory AKT activity during ibrutinib therapy.
Heat transfer, physiological responses, and subjective perceptions during short contact time with wood or other materials
Authors: Tsunetsugu Yuko , Sugiyama Masaki 
Published: March 2021

AbstractThis study investigates the physiological responses and subjective perceptions of touching wood. In particular, it focuses on their respective relationships with the amount of heat transfer across the hand–material interface during contact. The study participants included 55 university students (20 females and 35 males) who gave written informed consent. The participants’ blood pressure, pulse rate, and cerebral blood hemoglobin concentrations were measured continuously for 90 s while they gently held vertical bar-shaped specimens of Japanese cypress (Chamaecyparis obtusa), Japanese oak (Quercus crispula), polyethylene, and aluminum. The specimens also included wood with a surface coating. We measured subjective warmth and comfort as well as the heat flux between the palm and the surface of the material. The wooden materials were rated as significantly warmer compared to aluminum and polyethylene, regardless of the wood species (cypress or oak) or its coating; this result corresponds with smaller heat transfers in the wooden materials. Additionally, the wooden materials were more comfortable to hold as compared to the aluminum bar. Based on the changes in blood pressure, touching Japanese cypress and uncoated Japanese oak were interpreted to induce less physiological stress. Therefore, we can conclude that wood, with lower thermal conductivity, feels warm, and it causes relatively smaller physiological changes compared to other materials with higher thermal conductivity. Thus, they may present less physiological burdens when touched.