bluuebunny/crossref_metadata_2025_split huggingface dataset:
LUCAT1 as an oncogene in tongue squamous cell carcinoma by targeting miR‐375 expression
Authors: Zhang Kai , Wang Qibao , Zhong Bo , Gong Zuode 
Published: March 2021

AbstractEmerging studies suggested that lncRNAs play a crucial molecular role in cancer development and progression. LncRNA LUCAT1 has been proved as oncogenic molecular in lung cancer, glioma, osteosarcoma, renal carcinoma and oesophageal squamous cell carcinoma. However, its roles and function mechanisms in tongue squamous cell carcinoma (TSCC) are still unknown. We showed that the expression of LUCAT1 was up‐regulated in the TSCC cells and tissues and the higher LUCAT1 expression was associated with the poor overall survival (OS). Knockdown expression of LUCAT1 suppressed TSCC cell proliferation, cycle and migration. In addition, we demonstrated that miR‐375 overexpression inhibited the luciferase activity of LUCAT1 wild‐type and knockdown LUCAT1 promoted the miR‐375 expression in TSCC cell. Furthermore, we indicated that miR‐375 expression was down‐regulated in the TSCC cell lines and tissues and the lower expression of miR‐375 was associated with poor OS. The expression of miR‐375 was inversely correlated with LUCAT1 expression in the TSCC tissues. Knockdown LUCAT1 promoted TSCC cell proliferation, cell cycle and migration partly through regulating miR‐375 expression. In summary, this study suggested the tumorigenic effect of lncRNA LUCAT1 in TSCC cells by targeting miR‐375 expression.
RAGE: A potential therapeutic target during FGF1 treatment of diabetes‐mediated liver injury
Authors: Zheng Peipei , Tang Zonghao , Xiong Jun , Wang Beini , Xu Jingyu , Chen Lulu , Cai Shufang , Wu Chengbiao , Ye Libing , Xu Ke , Chen Zimiao , Wu Yanqing , Xiao Jian 
Published: March 2021

AbstractAs a serious metabolic disease, diabetes causes series of complications that seriously endanger human health. The liver is a key organ for metabolizing glucose and lipids, which substantially contributes to the development of insulin resistance and type 2 diabetes mellitus (T2DM). Exogenous fibroblast growth factor 1 (FGF1) has a great potential for the treatment of diabetes. Receptor of advanced glycation end products (RAGE) is a receptor for advanced glycation end products that involved in the development of diabetes‐triggered complications. Previous study has demonstrated that FGF1 significantly ameliorates diabetes‐mediated liver damage (DMLD). However, whether RAGE is involved in this process is still unknown. In this study, we intraperitoneally injected db/db mice with 0.5 mg/kg FGF1. We confirmed that FGF1 treatment not only significantly ameliorates diabetes‐induced elevated apoptosis in the liver, but also attenuates diabetes‐induced inflammation, then contributes to ameliorate liver dysfunction. Moreover, we found that diabetes triggers the elevated RAGE in hepatocytes, and FGF1 treatment blocks it, suggesting that RAGE may be a key target during FGF1 treatment of diabetes‐induced liver injury. Thus, we further confirmed the role of RAGE in FGF1 treatment of AML12 cells under high glucose condition. We found that D‐ribose, a RAGE agonist, reverses the protective role of FGF1 in AML12 cells. These findings suggest that FGF1 ameliorates diabetes‐induced hepatocyte apoptosis and elevated inflammation via suppressing RAGE pathway. These results suggest that RAGE may be a potential therapeutic target for the treatment of DMLD.
Dynamic changes in metabolic health status in Chinese adults: Multiple population‐based surveys in Shanghai, China
Authors: Yang Yihui , Li Yanyun , Pei Jianfeng , Cheng Minna , Xu Wanghong , Shi Yan 
Published: March 2021

AbstractAims/introductionMetabolic syndrome (MS) has been increasing worldwide. The secular change in MS components, however, remains unclear. This study aimed to examine the dynamic change in metabolic health status in Chinese adults.Materials and methodsThree population‐based surveys using multistage stratified sampling were performed in Chinese aged 35–74 years in Shanghai in 2002–2003 (n = 12,302), 2009 (n = 7,400), and 2017 (n = 19,023). MS was defined according to the Adult Treatment Panel III criteria for Asian‐Americans. Generalized Estimating Equations and Cochran‐Armitage Trend Test was used to assess the prevalence trend over the years.ResultsThe prevalence of MS doubled in Chinese adults over the period (P for trend < 0.001). The largest increase occurred in younger men. Among MS components, the prevalence of high waist‐circumference (HWC), high blood glucose (HBG) and high blood pressure (HBP) increased in all subjects, whereas the prevalence of high triglycerides (HTG) and low high‐density lipoprotein cholesterol (LHC) increased in men but decreased in women. The increase in HBP contributed most to elevated MS, followed by HBG and HWC, resulting in the HBP‐HBG‐HWC the most common cluster of MS components. Metabolically unhealthy overweight also grew over the period.ConclusionsMetabolic health status has been exacerbating in Chinese adults and may increase burden of non‐communicable diseases.
Curative value of underwater endoscopic mucosal resection for submucosally invasive colorectal cancer
Authors: Fukuda Hiromu , Takeuchi Yoji , Shoji Ayaka , Miyake Muneaki , Matsueda Katsunori , Inoue Takahiro , Waki Kotaro , Shimamoto Yusaku , Kono Mitsuhiro , Iwagami Hiroyoshi , Nakahira Hiroko , Matsuura Noriko , Shichijo Satoki , Maekawa Akira , Kanesaka Takashi , Yamamoto Sachiko , Higashino Koji , Uedo Noriya , Ishihara Ryu 
Published: March 2021

AbstractBackground and AimOccasionally, colorectal tumors without characteristics of deep submucosal invasion are found to be invasive upon pathological evaluation after endoscopic resection (ER). Because the resection depth for underwater endoscopic mucosal resection (UEMR) has not been clarified, we evaluated the feasibility of UEMR for pathologically invasive colorectal cancer (pT1‐CRC).MethodsWe retrospectively investigated data on the backgrounds and outcomes of patients with pT1‐CRC who underwent UEMR between January 2014 and June 2019 at our institute. As a reference standard, the backgrounds and outcomes of pT1‐CRCs that had undergone conventional EMR (CEMR) were also investigated.ResultsThirty‐one patients (median age, 68 years [range, 32–88 years]; 22 men [71%]) were treated with UEMR. Median lesion size was 17 mm (range, 6–50 mm). The endoscopic complete resection rate was 100%. The overall en bloc resection rate was 77%, and the VM0, HM0, and R0 resection rates were 81%, 58%, and 55%, respectively. In cases of pT1a (invasion <1000 μm)‐CRC (n = 14), the en bloc, VM0, and R0 resection rates were 92%, 100%, and 71%, respectively. Seventeen patients (five with risk factors for lymph node metastasis and 12 without) were followed up, and no local recurrence and distant metastasis were observed during the follow‐up period (median follow‐up period, 18 months [range, 6–62 months]) after UEMR. The outcomes of UEMR seemed to be comparable with those of CEMR (n = 32).ConclusionsThe VM0 rate of UEMR for pT1‐CRC, especially for pT1a‐CRC, without characteristics of deep submucosal invasion seems feasible.
The nutritional management of people living with amyotrophic lateral sclerosis: A national survey of dietitians
Authors: White Sean , Zarotti Nicolò , Beever Daniel , Bradburn Mike , Norman Paul , Coates Elizabeth , Stavroulakis Theocharis , White David , McGeachan Alexander , Williams Isobel , Hackney Gemma , Halliday Vanessa , McDermott Christopher ,   The HighCALS group
Published: March 2021

AbstractBackgroundPeople living with amyotrophic lateral sclerosis (ALS) face many challenges with respect to taking adequate nutrition. Growing evidence links weight loss with negative prognostic outcomes. We aimed to explore the practice of dietitians in the UK with regard to the nutritional management of ALS.MethodsA national online survey was disseminated via professional groups, social media and newsletters to UK healthcare professionals between September and November 2018. The survey examined the nutritional management of ALS. Dietitian responses are reported in the present study.ResultsIn total, 130 dietitians responded to the survey. Two‐thirds reported that ALS comprised less than 20% of their total patient caseload. Forty‐two percent reported that nutritional screening took place in their organisation. One‐half of dietitians reported that patients were referred for dietetic assessment at ‘about the right time’, although 44% reported referrals were made too late. The majority (83%) of dietitians used resting energy expenditure predictive equations not validated in ALS. When setting weight goals, dietitians reported most frequently recommending weight maintenance if the patient’s body mass index was 18.5–25 kg m−2 (72%), 25–30 kg m−2 (98%), and > 30 kg m−2 (79%). In addition, 43% reported that people with ALS were not weighed sufficiently frequently.ConclusionsAlthough the importance of early nutritional assessment is recognised, the timeliness of dietetic input and on‐going monitoring of nutritional status in ALS care might not currently be ideal. Dietitians report using energy requirement predictive equations and setting weight goals that may not promote positive outcomes. Further research is required to understand the optimal nutritional management of ALS.
Characterisation of worn WC tool using STEM‐EDS aided by principal component analysis
Authors: Lazar Isac , Lindvall Rebecka , Lenrick Filip , Bushlya Volodymyr , Ek Martin 
Published: March 2021

AbstractInterdiffusion and chemical reactions contribute to tool wear in metal machining. Increased understanding of these processes, through characterisation of worn tools, can facilitate design of more resilient materials through chemical and diffusional passivation. However, the unknown reaction conditions, the large number of elements, and the formation of interspersed phases makes for a complex analysis. Here, we demonstrate the use of scanning transmission electron microscopy and energy dispersive X‐ray spectroscopy for characterising the interaction layer between a titanium alloy and a cemented carbide tool. Principal component analysis is used to find chemical correlations and help separate signals from embedded phases. Crucially, we evaluate the required X‐ray count statistics from simulated spectrum images and theory prior to the experiment. We find no indications of intermediate phases between the original WC and the metallic W interaction layer. Furthermore, we find enrichment of minor constituents in the titanium alloy closest to the tool which alter the solubility of out‐diffusing species, suggesting strong interrelations between the diffusion processes.
Myofascial Pelvic Pain: An Overlooked and Treatable Cause of Chronic Pelvic Pain
Authors: Ross Vanessa , Detterman Carly , Hallisey Anastasia 
Published: March 2021

AbstractMyofascial pelvic pain is a common, nonarticular musculoskeletal disorder characterized by the presence of myofascial trigger points in the lower abdominal wall and/or pelvic floor muscles. Myofascial pelvic pain is involved in an estimated 22% to 94% of cases of chronic pelvic pain, which is one of the most common gynecologic conditions in the United States. Myofascial pelvic pain may exist independently or in conjunction with disorders such as vaginismus, dysmenorrhea, and endometriosis and is frequently a causative factor in sexual pain or dyspareunia. This article reviews the pathophysiology, assessment, and treatment options for myofascial pelvic pain, with a particular focus on trigger point injections. Increased recognition and treatment of this commonly overlooked diagnosis has the potential to improve care and outcomes for many patients suffering from chronic pelvic pain.
89% Vichy mineralizing water with hyaluronic acid is a well‐tolerated adjunct treatment that helps restore skin barrier function in dry skin‐related inflammatory dermatoses and post‐procedure skin care: A Canadian study
Authors: Hong Chih‐Ho , Schachter Jordana , Sutton Allison B. , Salsberg Jennifer M. , Li Monica K. , Humphrey Shannon D. , Dayeh Nour R. 
Published: March 2021

ABSTRACTIntroductionMinéral 89 (M89), comprised of 89% Vichy mineralizing water and hyaluronic acid, has been formulated to help strengthen and restore skin barrier.AimAssess tolerance and efficacy of M89 in post‐esthetic procedures and dry skin‐related facial dermatoses.MethodAdults post‐esthetic procedure or presenting with inflammatory dermatoses (47 subjects; mean age 40.9 ± 13.2 years; any Fitzpatrick or skin phototype), applied M89 for 4 weeks, once or twice daily, as an adjuvant treatment. Information on clinical signs and subject‐reported symptoms, skin characteristics, tolerance, and subject and investigator satisfaction were collected.ResultsFollowing 4 weeks of M89 use, significant decreases with complete resolution of erythema (27.6%), desquamation (29.8%), irritation (32%), and skin dehydration (35.8%), as compared to baseline signs and symptoms, were observed. Overall grading improvements for erythema (84.8%; p < 0.001), desquamation (91.7%; %; p < 0.003), irritation (91.7%; %; p < 0.015), and skin hydration (46.2%; p < 0.015) were noted. There was no significant improvement in papules and pustules. Evaluation of subjective signs demonstrated significant decreases in skin sensations such as burning (−73%; p < 0.0001), itching (−71%; p < 0.0001), stinging‐tingling (−66.7%; p < 0.0001), as well as in skin dryness (−60%; p < 0.0001). M89 texture was rated very pleasant by 90% of patients. Investigators assessed M89 tolerance to be either good or very good (93%), and satisfactory or highly satisfactory impact on patient's skin (91.5%).ConclusionM89 is a highly tolerable adjuvant treatment that significantly improved clinical signs and symptoms related to a compromised skin barrier in various facial dermatoses and post‐aesthetic procedures.
Initial Development of a Situation‐based Measure of Emerging Adults’ Social Competence in their Same‐gender Friendships
Authors: Kirmayer Miriam H. , Khullar Thomas H. , Dirks Melanie A. 
Published: March 2021

This paper describes the development of a situation‐based tool to assess emerging adults’ social competence with same‐gender friends, providing information about (1) challenges occurring in these relationships, (2) the behaviors used to manage these situations, and (3) the perceived effectiveness of these strategies. Undergraduates (N = 747; 409 women; Mage = 20.16, SD = 1.43) participated in five studies. Transgressions, conflicts of interest, and support situations emerged as key challenges, and emerging adults reported using aggressive, assertive, avoidant, and apologizing behaviors to manage these situations. In general, apologizing and assertive behaviors were judged more effective than aggressive or avoidant behaviors. Results yielded the Inventory of Friendship Challenges for Emerging Adults (IFCEA), which showed expected associations with measures of interpersonal behavior.
Growth of the harmful alga,Prymnesium parvum(Prymnesiophyceae), after gradual and abrupt increases in salinity
Authors: Richardson Emily T. , Patiño Reynaldo 
Published: March 2021

Prymnesium parvumis a euryhaline, toxin‐producing microalga. Although its abundance in inland waters and growth potential in the laboratory is reduced at high salinity (>20), the ability of inland strains to adjust their growth after long‐term residence in high salinity is uncertain. An inland strain ofP. parvummaintained at salinity of 5 in modified artificial seawater medium (ASM‐5) was subjected to the following treatments over five sequential batch culture rounds: ASM‐5 (control); modified ASM at salinity of 30, raised with NaCl; modified ASM at salinity incrementally increased to 30 with NaCl; and Instant Ocean®at salinity of 30 (IO‐30). Exponential growth rate (r) was reduced when salinity was increased from 5 to 30 in ASM but returned to control values during the second round. When salinity was incrementally increased, a reduction inrstill occurred when salinity reached 25‐30. Maximum density was reduced at salinity of 30 in ASM upon abrupt transfer or incremental increase, and compensation did not occur. Growth performance in IO‐30 was comparable to control values. In conclusion, (i) long‐term compensation for acute inhibitory effects of high salinity occurred forrbut not maximum density, (ii) incremental increases in salinity did not prevent growth inhibition, suggesting the existence of a salinity threshold of 25–30 for onset of salinity stress, and (iii) the presence of a seawater‐like salt mixture prevented growth inhibition by high salinity. These findings provide new insights onP. parvum's long‐term ability to adjust its growth in environments of different salinity and ionic composition.
Local tissue effects and peri‐implant bone healing induced by implant surface treatment: an in vivo study in the sheep
Authors: Rousseau Nicolas , Msolli Inès , Chabrand Patrick , Destainville Arnaud , Richart Olivier , Milan Jean‐Louis 
Published: March 2021

AbstractObjectiveThe aim of this study was to assess, through biological analysis, the local effects and osseointegration of dental implants incorporating surface micro/nanofeatures compared with implants of identical design without surface treatment.BackgroundKnown to impact bone cell behavior, surface chemical and topography modifications target improved osseointegration and long‐term success of dental implants. Very few studies assess the performance of implants presenting both micro‐ and nanofeatures in vivo on the animal models used in preclinical studies for medical device certification.MethodsImplant surfaces were characterized in terms of topography and surface chemical composition. After 4 weeks and 13 weeks of implantation in sheep femoral condyles, forty implants were evaluated through micro‐computed tomography, histopathologic, and histomorphometric analyses.ResultsNo local adverse effects were observed around implants. Histomorphometric analyses showed significantly higher bone‐to‐implant contact in the coronal region of the surface‐treated implant at week 4 and week 13, respectively, was 79.3 ± 11.2% and 86.4 ± 6.7%, compared with the untreated implants (68.3 ± 8.8% and 74.8 ± 13%). Micro‐computed tomography analyses revealed that healing patterns differed between coronal and apical regions, with higher coronal bone‐to‐implant contact at week 13. Histopathologic results showed, at week 13, bone healing around the surface‐treated implant with undistinguishable defect margins, while the untreated implant still presented bone condensation and traces of the initial drill defect.ConclusionOur results suggest that the surface‐treated implant not only shows no deleterious effects on local tissues but also promotes faster bone healing around the implant.
Three‐dimensional microvascular network reconstruction from in vivo images with adaptation of the regional inhomogeneity in the signal‐to‐noise ratio
Authors: Sugashi Takuma , Yuki Hiroya , Niizawa Tomoya , Takuwa Hiroyuki , Kanno Iwao , Masamoto Kazuto 
Published: March 2021

AbstractObjectiveQuantification of angiographic images with two‐photon laser scanning fluorescence microscopy (2PLSM) relies on proper segmentation of the vascular images. However, the images contain inhomogeneities in the signal‐to‐noise ratio (SNR) arising from regional effects of light scattering and absorption. The present study developed a semiautomated quantification method for volume images of 2PLSM angiography by adjusting the binarization threshold according to local SNR along the vessel centerlines.MethodsA phantom model made with fluorescent microbeads was used to incorporate a region‐dependent binarization threshold.ResultsThe recommended SNR for imaging was found to be 4.2–10.6 that provide the true size of imaged objects if the binarization threshold was fixed at 50% of SNR. However, angiographic images in the mouse cortex showed variable SNR up to 45 over the depths. To minimize the errors caused by variable SNR and a spatial extent of the imaged objects in an axial direction, the microvascular networks were three‐dimensionally reconstructed based on the cross‐sectional diameters measured along the vessel centerline from the XY‐plane images with adapted binarization threshold. The arterial volume was relatively constant over depths of 0–500 µm, and the capillary volume (1.7% relative to the scanned volume) showed the larger volumes than the artery (0.8%) and vein (0.6%).ConclusionsThe present methods allow consistent segmentation of microvasculature by adapting the local inhomogeneity in the SNR, which will be useful for quantitative comparison of the microvascular networks, such as under disease conditions where SNR in the 2PLSM images varies over space and time.
Analgesic Effects of Ultrasound‐Guided Iliohypogastric/Ilioinguinal Nerve Block Combined with Lateral Femoral Cutaneous Nerve Block in Total Hip Arthroplasty via Direct Anterior Approach: A Retrospective Cohort Study
Authors: Wang Qiuru , Yang Yong , Yang Zhouyuan , Hu Yunlian , Zhao Xin , Chen Changjun , Kang Pengde 
Published: March 2021

ObjectiveThis study aimed to explore the efficacy and safety of the combination of lateral femoral cutaneous nerve blocks (LFCNB) and iliohypogastric/ilioinguinal nerve blocks (IHINB) on postoperative pain and functional outcomes after total hip arthroplasty (THA) via the direct anterior approach (DAA).MethodsIn this retrospective cohort study, patients undergoing THA via the DAA between January 2019 and November 2019 were stratified into two groups based on their date of admission. Sixty‐seven patients received LFCNB and IHINB along with periarticular infiltration analgesia (PIA) (nerve block group), and 75 patients received PIA alone (control group). The outcomes included postoperative morphine consumption, postoperative pain assessed using the visual analogue scale (VAS), the QoR‐15 score, and functional recovery measured as quadriceps strength, time to first straight leg rise, daily ambulation distance, and duration of hospitalization. The Oxford hip score and the UCLA activity level rating were assessed at 1 and 3 months after surgery. In addition, postoperative complications were recorded. Patients were also compared based on the type of incision used during surgery (traditional longitudinal or “bikini” incision).ResultsPatients in the nerve block group showed significantly lower postoperative morphine consumption, lower resting VAS scores within 12 h postoperatively, lower VAS scores during motion within 24 h postoperatively, and better QoR‐15 scores on postoperative day 1. These patients also showed significantly better functional recovery during hospitalization. At 1‐month and 3‐month outpatient follow up, the two groups showed no significant differences in Oxford hip score or UCLA activity level rating. There were no significant differences in the incidence of postoperative complications. Similar results were observed when patients were stratified by type of incision, except that the duration of hospitalization was similar.ConclusionCompared to PIA alone, a combination of LFCNB and IHINB along with PIA can improve early pain relief, reduce morphine consumption, and accelerate functional recovery, without increasing complications after THA via the DAA.