bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of Listerin antigen as a tool for Diagnosis of listeriosis
Authors: Al - Khatib G. M. , Al - Haddad Zainab A.A. , Al- Hissen Sanaria Fawzi , Al- Kutbi Sahar Hassan 
Published: September 2021

This study was conducted to produce a specific antigen for detection L. monocytogenes infection. The antigen was prepared by culturing L. monocytogenes on suitable media and then disrupted by ultrasonicatorwaves.
The water – soluble extract of sonically disrupted Listeria were used for skin testing guinea pigs infected with (1*108 CFU / ml) of a L. monocytogenes. Two infected doses were used for immunization at intervals of (10) days between them.
Two skin tests were done, 10 days and 15 days after the 2nd infected dose. The skin test results were read after 24 & 48 hrs. which showed clear thickening differences and redness at the injection sites after 24 hrs. and become more clear after 48 hrs.
Role of some psychrotrophic aerobic sporeforming bacteria in spoilage of raw milk
Authors: عبد المنعم معتز عبد الواحد , الخزرجي نجم هادي , علي سلطان عماد ابراهيم 
Published: September 2021

This study was planned to isolated and identify some psychrotrophic aerobic sporeforming bacteria from raw milk and determine the predomenant isolate.
Microbiological estimations were performed for (100) raw milk samples collected from four sources (filters, stations, bulk tanks and milk containers) and for the same samples after heat treatment at 80°C for (12) minutes and upon subsequent storage at (7)°C for 0,7,14 and 21 days.
The Aerobic plate count (APC) was determined and the total bacterial count were compared with each of the mesophilic sporeformer count (MSFC) at (0) & (7) days of storage & the psychrotrophic sporeformer count (PSFC) upon storage at (7)°C for 0,7,14 and 21 days. Positive relationship existed between the increase in (APC) before heat treatment & the increase in (MSFC) at (0) or (7) days or (PSFC) at 0,7,14 & 21 days of storage at 7°C. Immediately after heat treatment at (80)°C for 12 minutes 59% of the samples had psychrotrophic sporeformer counts of <10 cfu/ml, but after (21) days of storage at (7)°C, 79% of the samples had counts of > 109 cfu/ml.
Bacillus cereus and Bacillus mycoides were identified as the predominant psychrotrophic sporeformers in the heated milk, stored at 7°C.
The results confirmed that growth of heat-resistant psychrotrophicsporeforming organisms co-incide with spoilage of heated milk, and was a higher than of the normal pasteurization temperatures or longer time which enhanced the germination of these spores. psychrotrophic sporeformers have been shown to be responsible for such off-flavors (bitter, stale, rancid and putrid) of heated milk stored at (7)°C for (21) days, and sweet curdling observed in 70% of the samples in the forms of "buttons” at the bottom of the containers after (21) days of storage at (7)°C.
Most of the isolated psychrotrophic B cercus were able to produce porteinase, lipases and lecithinase enzymes beside the haemolysin.
It was concluded that the number of (PSFC) in raw milk
conditions prevailing sanitary
the depends upon apparently during production and upon time and temperature of milk storage before processing.
Study of the pathological changes induced by irradiated protoscolices (from sheep hydatid cysts ) after intra peritoneal injection in white male mice
Authors: Faleh Enaam Bader , Majeed Saleh Kadhim , Al - Bayati Ahmed Jassim 
Published: September 2021

In the present study , we investigate the effect of Gamma radiation of wavelength 81.58416X10^-13 on the viability of protoscolices , the larval stage of Echinococcus granulosus , which was isolated from liver hydatid cysts of invested sheep.
The study was accomplished on 80 white male mice , the mice were divided randomly into four equal groups , group 3 and 4 were invested intra peritoneally with 2000 Gamma irradiated protoscolices, with 5 and 25 Gray of Gamma rays for 1.2 and 6.6 minutes respectively
The experimental irradiation of protoscolices was Gamma rays cobalt 60 ( Co-60 ). Then the ability of protoscolices was checked before and after the irradiation. Group 1 and group 2 were used as negative and positive control respectively.
After one month group 2 , 3 and 4 were given challenge dose of 2000 protoscolices intraperitoneally for every single mouse in every group. Mice were killed after 2,4,6,8, and 12 weeks after the challenge .
Macroscopic and microscopic studies were done for all experimental groups.
The following findings were seen :
1. Reduction of the violability of protoscolices by inverse of the radiation intensity incorrespondense with the time of exposure.
2. The macroscopic studies indicate reduction in the average number and diameter of the secondary hydatid cyst in the irradiated groups in comparison with the positive group.
3. The histopathological changes were characterized by presence of degenerated distracted hydatid cysts in the livers , especially in the irradiated group , with the presence of hyperplastic splenic white pulp lymphoid tissue in comparison with the positive control .
Metabolic, Biochemical and Histopathological Studies to Explore the Calcium Role in Antagonizing Gentamicin . Toxic Side Effect in Rats
Authors: Abbass Duraid Abdulhadi , Ubaid Khalid Ali 
Published: September 2021

Twenty rats were divided equally into four groups, housed separately in special metabolic cages. Animals of first, second and third group injected S.C. with gentamicin sulphate alone at a dose of 5mg/Kg (T) or as a mixture with calcium at a dose of 7.5 mg/Kg injected S.C. (T2) or separately with calcium given orally at a dose of 22.5 mg/Kg (T3). The fourth group considered as a control and injected S.C. with distilled water (C). The experiment includes samples analysis of sample of one week pre and post treatment and three weeks daily treatment.
The results showed significant changes in studied parameters proportional with treatment period in animals of first group (Ti) in comparison with other groups expressed metabolically as a decline in body weight, increase in daily urine volume, biochemical as increase in serum creatinine, BUN, AP, AST, as well as histopathological changes in kidney nephrons and tubules.
Calcium therapy whether as a mixture (T2) or alone (T3) gave nearly complete protection against gentamicin toxicity in the first and second week and incomplete one in the third week of treatment that could be attributed to the competitive interaction between calcium and gentamicin due to the similarity in charges and binding site that may cause a decline in gentamicin disposition in the target organ and tissue cell and so reduce its toxic side effect.
Cryptosporidiosis In Man And Animals In Al-Tameem Province/ Iraq.
Authors: Kadir Mohammed Abdul-Aziz 
Published: September 2021

This study was conducted on 790 stool samples from infants and young children up to ten years of age, suffering from acute diarrhea who attended Kirkuk Pediatric Hospital, from the beginning of August 1999 of July 2000. The animal study was carried on 160 lambs, 130 calves and 110 kids suffering from diarrhea in private veterinary clinics in Al-Tameem province.
The rate of acute cryptosporidiosis was (10%). There was no significant difference between the two sexes . It was higher in winter and spring than summer and autumn months .
The infection rate was higher among children in contact with poultry than those in contact with cattle and sheep and was lowest among those not in contacts with animals.
The distribution of cryptosporidiosis among domestic animals was as follows: in lambs, calves and kids the infection rates were 20%, 19.2% and 23.6% respectively.
Experimental infection of dogs with Echinococcus granulosus (Batsch, 1986) from protoscoleces of donkey's hydatid cyst
Authors: Rahif Raad Harbi , Atae Amal Hassan 
Published: September 2021

This study was conducted, for the 1st time in Iraq, to infect 5 puppies, 1 - 5 month old, with protoscoleces of hydatid cyst isolated from the liver of donkey, slaughtered at zoo abattoir of Al-Zawra park in Baghdad, at a dose rate of 10000 protoscoieces i puppy (n=2) and 20000 protoscoleces / puppy (n=3) for 35 and 40 days post infection. The dogs were killed and the developed Echinococcus granulosus were isolated from the small intestine. Results of the study revealed
variation in the period of maturation of the collected cestodes, and the mean total length of the mature worms at 35 days PI was 4.53+ 0.24 mm, and for those at 40 days PI was 7+ 0.20 mm, whereas for the gravid ones at the same age of the latter was 8.57 + 0.25 mm. Results found that infection rate with E. granulosus, which their strobilae composed from 4 proglottids at 35 days PI, was 29.03%, and with those, which their strobilae composed from 5 proglottids at 40 days PI, the rate was 0.76%. It was found that the mean length of the gravid proglottid equal to or longer than half the total worm length with a ratio of 1.77 + 0.05 for those at 35 days of age, and 2.2 + 0.35 and 1.93 + 0.01 for mature and gravid cestodes at the age of 40 days.
Pembelajaran Berbasis Masalah: Pengoptimalan Media Online Melalui Pembelajaran Berjarak
Authors: Bayu Dwi Prabowo Heri 
Published: September 2021

The world is being rocked by the covid-19 pandemic outbre ak. The pandemic effect on all human activities as a whole, whitout exception the education world. Education as an one of the heart of the nation’s solid survival must be save under these conditions, including education in Indonesia. Since the implementation of learning at home by the goverment on March, 16, 2020, learning patterns have changed in such a way. The question is, what kind of learning is effective in the present? In this case, the problem to be answered will be examined with the idea of distance learning (PJJ) through online media in an effort to keep the learning and learning process going. This research uses library research, while the source of data is obtained through a variety of relevant literature. In this case, the researcher uses theory of problem based learning and distance learning. As a result, the learning process at this time still needs to be intensively studied, improved and further developed. In addition, improving the format of education is a shared responsibility of all elements of society, working hand in hand and supporting the resilience and effectiveness of learning at all levels of education is a necessity.
Complications of X-ray Endovascular Interventions in Patients with Coronary Heart Disease with Reduced Left Ventricular Systolic Function
Authors: Aksenov Yevhenii V. , Demchenko Ruslan B. 
Published: September 2021

The work is dedicated to the research of direct results and immediate possible complications of interventions in X-ray surgery with coronary stent implantation. Our patients with chronic coronary syndrome and reduced left ventricular ejection fraction (LVEF) of less than 40% were studied. The study included 112 patients who were divided into two groups. The first group included 51 patients with LVEF <40% (mean value 32.8 ± 1.14%), the second group involved 61 patients with LVEF >40% (mean value 59.4 ± 1.02%). The mean age of the subjects ranged from 59 to 74 years (mean value 63.85 ± 1.73 years).
There were no gender-related differences among the selected patients. There was no statistical difference in comorbidities in both groups. Both groups of patients were examined and managed according to the protocol of diagnosis and treatment adopted at the National Amosov Institute of Cardiovascular Surgery for patients suffering from chronic coronary syndrome.
The study results show that patients with coronary artery disease and reduced LVEF 2 times more often developed complications in early postoperative period after PCI with revascularization through stent placement than in group of patients with preserved contractile myocardial function. Duration of PCI procedure and contrast agent use was 26.3±1.4% and 29.8±4.2% higher, respectively; the frequency of life-threatening arrhythmias was 54% higher. Intraoperational signs of acute heart failure developed 4.8 times less often in the control group than in the experimental group. In general, all the patients after stenting achieved good anti-ischemic effect.
The Influence of the Duration of Acute Coronary Syndrome on the Outcomes of Endovascular Treatment
Authors: Salo Sergii V. 
Published: September 2021

Multicenter studies have proven the high effectiveness of percutaneous coronary intervention (PCI) in terms of restoring patency of the infarct-related artery (IRA) and improving the prognosis in acute myocardial infarction (AMI). The mechanism of improvement of clinical result after PCI procedure appears to be multifactorial.
The aim. To investigate the effect of IRA stenting on the clinical course, prognosis and contractility of the heart in patients with different duration of acute myocardial infarction and its influence on the short-term and long-term effects after intervention. The main determinant for the favorable clinical course and improvement of the prognosis is early (within the first hours of the disease) restoration of antegrade blood flow by IRA stenting. Thus, it is possible to signifi-cantly improve the blood supply to the peri-infarct zone and limit the area of necrosis and maintain heart rate.
Materials and methods. The analyzed group included 684 patients with AMI who were endovascularly treated at the Department of Emergency Endovascular Heart Surgery of the National Amosov Institute of Cardiovascular Surgery of the National Academy of Medical Sciences of Ukraine from January 1, 2017 to January 1, 2021. Coronary artery stenting was performed in all the patients. STEMI occurred in 495 (72.4%) patients, and non-STEMI in 189 (27.6%) patients.
The mean age of the subjects was 61.8 ± 12.1 years. There were 289 women (42.3%) and 395 men (57.7%). Distribu-tion of the patients depending on the initial Killip class was as follows: 13 (1.9%) had class I, 199 (29.1%) had class II, and 472 (69.0%) had class III myocardial infarction. Atrial fibrillation occurred in 72 (10.5%) patients. Echocardiographic parameters were as follows: left ventricular (LV) end-systolic index 54.1 ± 12.8 ml/m2, LV ejection fraction 0.53 ± 0.05, left atrial diameter 39.5 ± 3.8 mm, systolic pulmonary artery pressure 44.8 ± 7.8 mmHg.
In this study, clinical condition and functional capacity of the heart muscle in patients with AMI depending on the condition of the stent segment and the timing of endovascular procedures after the onset of the disease were first inves-tigated in the long term.
Conclusions. High efficiency and safety of PCI have been proven, which makes it possible to recommend this pro-cedure for wide application. It has been proven that PCI using matrix and modular stents, as well as statins can reduce the frequency of in-stent stenosis and improve the clinical course of the disease in the long term. It has been proven that stenting in patients with AMI is most effective in the earliest stages of the disease with preservation of LV contractility with possibly complete myocardial revascularization, which contributes to the preservation of viable myocardium in the peri-infarct zone, improvement of myocardial contractility and prevention of myocardium remodeling.
Substrate Modification in Catheter Treatment of Atrial Fibrillation
Authors: Stychynskyi Oleksandr S. , Topchii Alina V. , Almiz Pavlo O. 
Published: September 2021

According to modern concepts, atrial fibrillation (AF) occurs when there are triggers affecting the prepared substrate (atrial myocardium) in the presence of modulating factors that contribute to the occurrence of arrhythmia. Catheter treatment of AF has been most successfully developed in the field of affecting triggers (since late 1990s, the most successful was a technique of isolation of pulmonary veins which are the main source of trigger impulses in AF). Over the past two decades, various techniques have also been proposed for influencing the fibrous substrate.
The aim. To analyze the most advanced techniques for influencing the fibrous substrate during catheter treatment of AF.
Materials and methods. We analyzed the experience of leading electrophysiological centers in this field.
Discussion. Modern studies contain various electrophysiological criteria of fibrous myocardium. However, the signal amplitude less than 0.5 mV is considered borderline between healthy and damaged tissues by most authors. The task of the catheter action on the myocardium is to separate the fibrously altered tissue and intact tissue. This can be achieved by isolating the area of fibrosis or by transforming it into a scar tissue incapable of arrhythmogenesis. It should be noted that both methods are associated with the same frequency of the absence of AF paroxysms, which can be regarded as confirmation of the advisability of influencing the substrate. The most important is that exposure of the substrate can significantly reduce the recurrence rate of AF compared to that when the ablation procedure is limited to isolation of the pulmonary veins.
Conclusions. Modern methods of influencing the areas of fibrosis in the atria can significantly improve the results of catheter treatment of AF.
Sosialisasi Pembuatan Tanaman Obat Keluarga (TOGA) pada Masyarakat Kepulauan Lemukutan Kalimantan Barat dalam Usaha Penanggulangan Penyakit Demam Berdarah Dengue (DBD)
Authors: Helena Shifa , Lestari Desriani , Arbiastutie Yanieta , Warsidah Warsidah 
Published: September 2021

Demam Berdarah Dengue (DBD) adalah penyakit yang disebabkan oleh virus Dengue, banyak terjadi di wilayah tropis ataupun subtropis. DBD banyak berjangkit pada anak usia 5-16 tahun, dengan gejala demam tinggi, perdarahan lalu syok berujung pada kematian dalam waktu singkat. Usaha pencegahan adalah dengan pembersihan genangan air, pengurasan atau fogging, penggunaan anti nyamuk bakar, lotion, fogg terutama pagi dan sore hari. Jika positif terjangkit segera dirawat di rumah sakit serta mengkonsumsi obat-obatan penurun demam, bahan alam yang dapat meningkatkan kadar trombosit darah serta banyak minum air. Tanaman Obat Keluarga yang disingkat TOGA adalah sejumlah tanaman yang dipelihara di pekarangan, berkhasiat obat, dapat memenuhi keperluan obat-obatan sekeluarga dan mewujudkan kemandirian keluarga dalam pengobatan penyakit. Zodia, sere wangi, daun selasih dan lavender merupakan tanaman pengusir nyamuk karena bau yang tidak disukai oleh nyamuk, dapat digosokkan langsung ke kulit atau menempatkan pot tanaman tersebut di area aktivitas kita. Kegiatan pengabdian kepada masyarakat (PKM) ditempatkan di Pulau Lemukutan Kalimantan Barat untuk mensosialisasikan pembuatan TOGA khususnya tanaman untuk pencegahan dan pengobatan penyakit DBD. Kegiatan diikuti oleh 25 orang warga, selanjutnya akan menjadi duta-duta TOGA di seluruh kepulauan Lemukutan.
Peningkatan Pengetahuan Ibu Hamil terhadap Senam Hamil sebagai upaya mengurangi kejadian Ruptur Perineum
Authors: Nikmah Khusnul , Ningsih Eka Sarofah , Yushofa Vita 
Published: September 2021

Senam hamil merupakan suatu bentuk latihan guna memperkuat dan mempertahankan elastisitas otot-otot dinding perut, ligament-ligament, otot dasar panggul yang berhubungan dengan proses persalinan. Persalinan seringkali mengakibatkan robeknya perineum atau ruptur perineum. Persalinan dengan ruptur perineum apabila tidak ditangani secara efektif menyebabkan perdarahan dan infeksi menjadi lebih berat, serta pada waktu panjang dapat mengganggu ketidaknyamanan ibu dalam berhubungan seksual. Salah satu hal untuk mencegah terjadinya ruptur perineum yaitu dengan menjaga keelastisan perineum. Peningkatan elastisitas perineum dapat dilakukan dengan senam hamil. Mengetahui Peningkatan Pengetahuan Ibu Hamil terhadap Senam Hamil sebagai Upaya Mengurangi Kejadian Ruptur Perineum di BPM Miftahul Khoiriyah Surabaya. Metode yang digunakan dalam pelaksanaan kegiatan pengabdian masyarakat ini adalah dalam bentuk ceramah atau penyuluhan dan diskusi atau tanya jawab. Dengan kita memberikan penyuluhan dan melakukan senam hamil diharapkan pengetahuan ibu hamil dapat meningkat dan akan mencegah terjadinya rupture pada persalinan, hal ini sangat penting sekali untuk disosialisasikan kepada ibu - ibu hamil yang lain supaya lebih bersemangat jika hamil lebih baik melakukan senam hamil. Maka dari itu kita sebagai tenaga kesehatan (Bidan) harus memberikan penyuluhan dan motifasi yang banyak kepada pasien untuk melakukan senam hamil selama masa kehamilan agar memperkuat dan mempertahankan elastisitas saat mengejan pada waktu bersalin.
Sosialisasi SADARI (Pemeriksaan Payudara Sendiri) sebagai upaya awal pencegahan Ca Mamae
Authors: Darwati Lilik , Nikmah Khusnul , Aziz Mersya Nur Avivah 
Published: September 2021

Ca mamae merupakan suatu jenis penyakit berupa pertumbuhan jaringan yang tidak terkendali kerena hilangnya mekanisme kontrol sel sehingga pertumbuhan menjadi tidak normal. Kegiatan penyuluhan diawali dengan pre test kemudian dilanjutkan dengan pemberian materi serta pemutaran video terkait ca mamae, dan simulasi atau praktik secara langsung tehnik Kegiatan penyuluhan diawali dengan pre test kemudian dilanjutkan dengan pemberian materi serta pemutaran video terkait ca mamae, dan simulasi atau praktik secara langsung tehnik SADARI (Periksa payudara sendiri) sebagai salah satu tehnik untuk deteksi dini ca mamae,. Kemudian melakukan diskusi tanya jawab dan diakhiri dengan post test sebagai evaluasi kegiatan penyuluhan. Kegiatan dipandu oleh narasumber sebagai fasilitator dengan dibantu peralatan dan perlengkapan pendukung pelatihan seperti leaflet, video, pantoom dan media visual/LCD. untuk mengevaluasi keterampilan peserta dalam melakukan tehnik SADARI, di evaluasi dengan melihat kemampuan peserta ibu fatayat secara langsung dalam mensimulasikan tehnik SADARI. Saat diskusi tanya jawab, disimpulkan ada keinginan dan antusias dari ibu fatayat muslimat untuk mengetahui lebih dalam tentang kanker payudara dan penatalaksanaan pengobatan, perawatan dan penegakan diagnosis, tidak hanya sebatas deteksi dini saja. hasil evaluasi kegiatan dengan bentuk penyuluhan dan untuk meningkatkan pengetahuan ibu fatayat tentang deteksi dini ca mamae. Setelah di beri penyulihan terjadi input kognitif pada peserta lalu perubahan konseptual dan setelah itu itu terdapat output, sehingga peserta dapat memahami pembelajaran dan peserta dapat menjawab soal postest dengan baik. Selain metode penyuluhan dalam bentuk video juga menjadi salah satu daya tarik sehingga peserta lebih antusias saat mengikuti materi penyuluhan.