bluuebunny/crossref_metadata_2025_split huggingface dataset:
A4GALT Mediated the Glycosylation of B7-H3 in Human Colorectal Cancer Cell Lines
Authors: Li Fuchao , Han Dan , Chen Mengxi , Li Jingmei , Yang Li , Wang Weipeng , Yu Chenggong 
Published: December 2021

Abstract
B7-H3 is one of the most important members of the B7/CD28 family, and its expression level is abnormally high in a variety of tumors. B7-H3 inhibits T cell activation via binding to the corresponding receptors on T cells, thereby mediating tumor immune escape. Glycosylation is a common post-translational modification of proteins, which plays an essential role in protein expression patterns and biological functions. Current evidence has shown that the abnormal glycosylation of B7-H3 in tumors is of great significance for protein expression and ligand-receptor binding. Therefore, in-depth exploration of the underlying mechanism of glycosylation modification of B7-H3 is expected to provide new insights for tumor immunotherapy. To investigate the underlying mechanism of glycosyltransferase-mediated glycosylation of B7-H3. Firstly, the CHIPBase database was used to screen glycosyltransferases with a high correlation with the protein expression of B7-H3. Then their siRNAs were designed and synthesized to transfect into cells, and the western blotting assay was performed for further screening. Secondly, the siRNA and overexpression plasmid of the screened glycosyltransferase were respectively transfected into cells to verify the effect on the expression level of B7-H3. Thirdly, the effect of glycosyltransferase on the expression level of B7-H3 was explored by changing its substrate level. Finally, the co-immunoprecipitation experiment was conducted to verify whether protein binding existed between B7-H3 and the glycosyltransferase. The glycosyltransferase called A4GALT had the highest correlation with the protein expression of B7-H3. After knocking down or overexpressing A4GALT, the protein expression level of B7-H3 both changed significantly. When knocked down GALT to reduce the galactosyl donors, B7-H3 was significantly down-regulated. And B7-H3 was up-regulated while increasing the galactose in the medium. In addition, the Co-immunoprecipitation experiment proved that there was protein binding between B7-H3 and A4GALT. A4GALT can positively regulate the expression of B7-H3, and changing the level of galactosyl donors can also positively regulate the expression of B7-H3. There is a protein interaction between A4GALT and B7-H3.
Reference genome-assisted identification of stem rust resistance gene Sr62 encoding a tandem kinase
Authors: Yu Guotai , Matny Oadi , Champouret Nicolas , Steuernagel Burkhard , Moscou Matthew J. , Hernández-Pinzón Inmaculada , Green Phon , Hayta Sadiye , Smedley Mark , Harwood Wendy , Kangara Ngonidzashe , Yue Yajuan , Gardener Catherine , Banfield Mark J. , Olivera Pablo D. , Welchin Cole , Simmons Jamie , Millet Eitan , Minz-Dub Anna , Ronen Moshe , Avni Raz , Sharon Amir , Patpour Mehran , Justesen Annemarie F. , Jayakodi Murukarthick , Himmelbach Axel , Stein Nils , Wu Shuangye , Poland Jesse , Ens Jennifer , Pozniak Curtis , Karafiátová Miroslava , Molnár István , Doležel Jaroslav , Ward Eric R. , Reuber T Lynne , Jones Jonathan D. G. , Mascher Martin , Steffenson Brian J. , Wulff Brande B. H. 
Published: December 2021

Abstract
The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. We developed a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and used positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which was also transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines showed high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
Zeolite-X Encapsulated Ni(II) and Co(II) Complexes with 2,6-Pyridine Dicarboxylic Acid as Catalysts for Oxidative Degradation of Atenolol in an Aqueous Solution
Authors: Hassani Fatemeh , Sharif Mahboubeh A. , Tabatabaee Masoumeh , Mahmoodi Mahboobeh 
Published: December 2021

Abstract
Complexes of Co (II) and Ni (II) with dipicolinic acid, 2,6-pyridine dicarboxylic acid (PydcH2) have been synthesized in the NaX (zeolite-X) nanopores. The formation of zeolite X encapsulated Co(II) and Ni(II) complexes ([M(pydcH)2]-NaX, where M = Co(II) and Ni(II]) were confirmed using spectroscopic methods of FT-IR, elemental analysis, XRD, FE-SEM, and TEM. It was affirmed that the encapsulation of complexes in NaX pores was performed without changes in the structure and shape of the zeolite. The oxidative degradation reaction of atenolol with hydrogen peroxide as an oxidant was performed in the presence of synthesized [M(pydcH)2]-NaX nanocomposites to study their catalytic activity. Therefore, oxidation of atenolol was performed under different conditions of catalyst, temperature, and time. Under optimal conditions, catalysts [Co(pydcH)2]-NaX and [Ni(pydcH)2]-NaX showed 82.3% and 71.1% activity of atenolol oxidation, respectively. These catalysts were stable after recovery and were used three more times. The results showed that these catalysts were reusable and had a reduction in the catalytic activity of less than ten percent.
BNT162b2 boosted immune responses six months after heterologous or homologous ChAdOx1nCoV-19/BNT162b2 vaccination against COVID-19
Authors: Behrens Georg , Barros-Martins Joana , Cossmann Anne , Ramos Gema Morillas , Stankov Metodi , Odak Ivan , Dopfer-Jablonka Alexandra , Hetzel Laura , Köhler Miriam , Patzer Gwendolyn , Binz Christoph , Ritter Christiane , Friedrichsen Michaela , Schultze-Florey Christian , Ravens Inga , Willenzon Stefanie , Bubke Anja , Ristenpart Jasmin , Janssen Anika , Ssebyatika George , Bernhardt Günter , Hoffmann Markus , Pöhlmann Stefan , Krey Thomas , Bosnjak Berislav , Hammerschmidt Swantje , Forster Reinhold 
Published: December 2021

Abstract
Reports suggest that COVID-19 vaccine effectiveness is decreasing, either due to waning immune protection, emergence of new variants of concern, or both. Heterologous prime/boost vaccination with a vector-based approach (ChAdOx-1nCov-19, ChAd) followed by an mRNA vaccine (e.g. BNT162b2, BNT) appeared to be superior in inducing protective immunity, and large scale second booster vaccination is ongoing. However, data comparing declining immunity after homologous and heterologous vaccination as well as effects of a third vaccine application after heterologous ChAd/BNT vaccination are lacking. We longitudinally monitored immunity in ChAd/ChAd (n=41) and ChAd/BNT (n=88) vaccinated individuals and assessed the impact of a second booster with BNT in both groups. The second booster greatly augmented waning anti-spike IgG but only moderately increased spike-specific CD4+ and CD8+ T cells in both groups to cell frequencies already present after the boost. More importantly, the second booster efficiently restored neutralizing antibody responses against Alpha, Beta, Gamma, and Delta, but neutralizing activity against B.1.1.529 (Omicron) stayed severely impaired. Our data suggest that inferior SARS-CoV-2 specific immune responses after homologous ChAd/ChAd vaccination can be cured by a heterologous BNT vaccination. However, prior heterologous ChAd/BNT vaccination provides no additional benefit for spike-specific T cell immunity or neutralizing Omicron after the second boost.
Characterization of Virulence Factors in Enterotoxin-Producing Staphylococcus Aureus From Bulk Tank Milk
Authors: Jung Hye-Ri , Lee Young Ju 
Published: December 2021

Abstract
Background: Staphylococcus aureus, a persistent and chronic mastitis-causing pathogen, produces various virulence factors, including enterotoxins. This study analyzed the genetic characteristics of bovine mastitis-related virulence factors to evaluate potential pathogenesis in S. aureus isolated from bulk tank milk.Results: Among 93 S. aureus isolates from 396 dairy farms in six factories operated by three dairy companies in Korea, 40 (43.0%) isolates carried at least one or more enterotoxin genes and there were significant differences between factories within the same company (p < 0.05). Moreover, S. aureus carrying enterotoxin genes showed a higher prevalence in all virulence genes tested in this study except for pvl and lukM, which were not detected in any isolate, than the isolates without enterotoxin genes. In particular, the prevalence of six genes (hla, hlb, lukED, fnbA, clfA, and clfB) was significantly higher in S. aureus carrying enterotoxin genes than isolates without enterotoxin genes (p < 0.05). The most common multilocus sequence type (ST) of 40 enterotoxin-producing isolates was ST188, and all isolates of ST188 harbored the see gene. However, none of the isolates of ST1 and ST72 carried the see gene, and all isolates of ST1 carried the seh gene.Conclusions: Although S. aureus isolated from bulk tank milk, not from mastitis, had a high prevalence of enterotoxins and virulence factors simultaneously, posing a public health threat. Moreover, high enterotoxins in bulk tank milk may be reflected by poor hygiene; therefore, it is important to develop strong monitoring and sanitation programs to ensure that dairy factories produce hygienic milk.
Characterization of a New Fiber From Cyperus Dichrostachus A.Rich Plant
Authors: GELAW(Lecturer) BELETE BAYE , Tesfaye(D.r) Tamrate 
Published: December 2021

Abstract
Natural fibers are of the good substitute sources for swapping synthetic fibers and reinforcing polymer matrices because of their contributions in maintaining of ecology, low energy requirement for processing and sustainability. The aim of this study is to characterize new fiber from Cyperus Dichrostachus A.Rich (CDA) plant. The CDA plant is a perennial non woody grass found in Ethiopian high lands and river basins. The fiber from this plant has good chemical composition of Cellulose (60.27%), hemicellulose (22.72%), lignin (16.59%) contents. It is light fiber having a density of 1010kg/m3 and good tenacity behaviour of 105.76cN/Tex with low elongation of 4.88%. The thermal stability of Cyperus Dicrostachys A,Rich fiber (CDAF) was studied using TGA and DTG analysis and revealed that the cellulose degraded at a temperature of 377.1°C. Fourier transform-infrared spectroscopy analysis confirmed that CDAF is rich in cellulose content. Furthermore, the properties of CDAF ensured that it can play a vital role as new reinforcement material and best alternative in bio composite industries. This will give competitive advantages when evaluated with other natural fibers reveals that there are significant potential benefits in implementation of “cleaner production” in textile material production industries. Specifically, replacement of synthetic fiber source with renewable biomass will reduce the environmental impact of these fibers. The future study will entail on investigating the possible valorization route especially in paper board, composite reinforcement and bio composite applications.
Improving statistical prediction and revealing nonlinearity of ENSO using observations of ocean heat content in the tropical Pacific
Authors: Seleznev Aleksei , Mukhin Dmitry 
Published: December 2021

Abstract
It is well-known that the upper ocean heat content (OHC) variability in the tropical Pacific contains valuable information about dynamics of El Niño–Southern Oscillation (ENSO). Here we combine sea surface temperature (SST) and OHC indices derived from the gridded datasets to construct a phase space for data-driven ENSO models. Using a Bayesian optimization method, we construct linear as well as nonlinear models for these indices. We find that the joint SST-OHC optimal models yield significant benefits in predicting both the SST and OHC as compared with the separate SST or OHC models. It is shown that these models substantially reduces seasonal predictability barriers in each variable – the spring barrier in the SST index and the winter barrier in the OHC index. We also reveal the significant nonlinear relationships between the ENSO variables manifesting on interannual scales, which opens prospects for improving yearly ENSO forecasting.
WITHDRAWN: Curved, Expanded and Inclined Diffraction Patterns of Grating--Rotating Grating Around Three Axes (1)
Authors: Peng Hui 
Published: December 2021

Abstract

The orientation-dependence of the interference/diffraction patterns of the 1D-double slit/1D-grating and 2D-cross-double slit/2D-cross-grating experiments have been studied experimentally and theoretically. However, the above experiments were limited to certain orientations, namely rotating around either one axis or two axes. In this article, the 3-axis-rotation apparatus is proposed/made, which can rotate the 1D-double slit/2D-cross-double slit and 1D-grating/2D-cross-grating, CW and CCW respectively, 00-3600 around three axes independently and sequentially. By this apparatus, the orientation-dependence of the patterns is systematically studied. Moreover, the experiments can be performed easily. Then we show that the photons, before landing on the detector/screen, behave as particles. The above observed phenomena provide the comprehensive information to theoretical study of the double slit/grating experiments. We suggest that the complete mathematical model should contain three rotation angles as parameters. Furthermore, the phenomena have potential applications.
Single Nucleotide Polymorphism Analysis of Pvmdr-1 in Plasmodium Vivax Isolated from Military Personnel of Republic of Korea in 2016 and 2017
Authors: Bong Jin-Jong , Lee Wonsig , Park Qu-Ehn , Noh Kyung Tae 
Published: December 2021

Abstract
Background: Malaria chemoprophylaxis using chloroquine (CQ) and primaquine (PQ) has been administered to resident soldiers in the 3rd Army of Republic of Korea (ROK) to prevent malaria infection since the year 1997. Due to mass chemoprophylaxis against malaria, concern exists about occurrence of chloroquine resistance (CQR). Herein, we investigated the single nucleotide polymorphisms (SNPs) of the Plasmodium vivax multi-drug resistance protein-1 (pvmdr-1) gene to monitor the risk of CQR. Methods: SNPs of the pvmdr-1 gene were analyzed in 73 soldiers of the 3rd Army of ROK diagnosed with infection by Plasmodium vivax (P. vivax). Results: Quintuple mutations (G698S, L845F, M908L, T958M, and F1076L) were detected in 73 soldiers. Mutation in the Y541C position was firstly detected in soldiers at a frequency of 1.3% (1/73). In addition, synonymous mutations were detected at positions K44, L493, T529, and E1233. Based on these SNPs, pvmdr-1 sequences of ROK were classified into 6 haplotypes. The phylogenetic analysis closed to Type of North Korean showed that P. vivax malaria of ROK could be a reason of influx from North Korea. In this study, there was no therapeutic resistance (CQ-mediated parasite clearance within 72 hours) for clinical samples that possessed various SNPs of pvmdr-1. Various SNPs including a newly identified non-synonymous mutation (Y541C) had been introduced into P. vivax malaria-endemic areas in ROK. Conclusions: Our study showed that synonymous and non-synonymous mutations of pvmdr-1 were introduced to the malaria chemoprophylaxis-executed regions of ROK from 2016 to 2017. Thus, to prevent the emergence of CQR, continuous surveillance for SNPs of pvmdr-1 related with CQR in the malaria-endemic regions of ROK is essential.
Refractory Craniopharyngioma: Successful Treatment with LINAC Based Stereotactic Radiosurgery(SRS): First Case in Nepal
Authors: Thakur Subhash , Chawla Saloni 
Published: December 2021

Abstract
Purpose: To present first case of refractory craniopharyngioma treated successfully with SRS in Nepal.Background: Craniopharyngioma is a benign tumour, which progresses slowly and compresses the pituitary gland and nearby structures. First line of treatment is surgery followed by adjuvant radiotherapy as complete resection is usually not feasible. Here, we are reporting a case of recurrent craniopharyngioma treated with LINAC based SRS.Case Presentation: A 43 years old man diagnosed case of craniopharyngioma in May 2019. He underwent left pterional craniotomy and subtotal resection of tumour and kept on observation. He developed symptomatic as well as radiological recurrence in July 2020. Second debulking was not possible, so we did SRS on 23rd August 2020; 14Gy Dose was delivered to gross tumour volume. Six months after SRS, Patient is doing well.Conclusions: LINAC based SRS is a frameless, non-invasive and safe procedure with excellent clinical outcomes for recurrent or residual craniopharyngioma.
Efficacy of Platelet-rich Plasma Impregnation for Unidirectional Porous Β-tricalcium Phosphate in Lateral Lumbar Interbody Fusion: Study Protocol for a Randomized Controlled Trial
Authors: Sato Kosuke , Funayama Toru , Noguchi Hiroshi , Asada Tomoyuki , Kono Mamoru , Eto Fumihiko , Shibao Yosuke , Miura Kosei , Kikuchi Naoya , Yoshioka Tomokazu , Takahashi Hiroshi , Koda Masao , Yamazaki Masashi 
Published: December 2021

Abstract
BackgroundPlatelet-rich plasma has been increasingly used in spinal fusion surgery. However, the efficacy of platelet-rich plasma in lateral lumbar interbody fusion is unclear. In Japan, Affinos® (Kuraray Co., Tokyo, Japan), which is a β-tricalcium phosphate artificial bone, has been increasingly used for spinal fusion as a bone graft. The purpose of this trial is to demonstrate whether Affinos® impregnated with platelet-rich plasma can achieve a higher fusion rate, more rapid fusion, and better clinical outcomes than Affinos® alone.Methods/DesignThe current study is a prospective randomized controlled trial. The current trial will include consecutive patients scheduled for lateral lumbar interbody fusion. Since an intervertebral cage for lateral lumbar interbody fusion has two spaces for a bone graft, two bone grafts are inserted for each intervertebral level. In the current study, an artificial bone with plate-rich plasma will be inserted into one space and an artificial bone without platelet-rich plasma will be inserted into the other space. We will compare the fusion rates between the bone graft with and without platelet-rich plasma. Our primary endpoint will be the interbody fusion rate at 1 year after surgery. DiscussionThe current trial will verify the efficacy of platelet-rich plasma with Affinos® for bony fusion in lateral lumbar interbody fusion. This trial will provide substantial evidence for the effectiveness and safety of platelet-rich plasma in spinal fusion surgery.Trial registrationJapan Registry of Clinical Trials (jRCT), ID: jRCTb032200199. First registered on 13 November 2020, https://jrct.niph.go.jp/latest-detail/jRCTb032200199. jRCT is approved as a member of the Primary Registry Network of WHO ICTRP.
Increase of Non-Albicans Candida Species and Their Antifungal Susceptibility in Intensive Care Unit Patients (Mexico)
Authors: Calvillo-Medina Rosa Paulina , Mejía-Romero Rocio Alejandrina , Martínez-Neria Magda , Olalde-Elias Juan José , Domínguez-Márquez Fernando 
Published: December 2021

Abstract
In Mexico little is known about candidemia by non-albicans Candida species and regarding their antifungal susceptibility. Besides without antifungal tests, fluconazole is one of the most used in empirical therapy. In the present study, we included patients from intensive care unit of one hospital in Mexico (2019–2020) with compatible yeast infection clinical signs, symptoms. Based on cultivable isolates, yeasts were identified by automated instrument and by molecular method (PCR), and their susceptibilities to six antifungals were characterized at different concentrations. From 105 patients, yeast cultures were recovered and identified mainly non-albicans Candida species (57.2%); and the most prevalent was C. glabrata (41.9%). Followed by C. albicans, C. krusei, C. parapsilosis, C. tropicalis and Cryptococcus neoformans. The most common infection site was urine (56%), followed by the bronchial aspirate (30%). Mostly the isolated fungi were susceptible to 5-flucytosine (98%) and to amphotericin B. Mainly C. glabrata followed by C. krusei and C. tropicalis were resistant to different concentrations of itraconazole, miconazole, and fluconazole. The present investigation contributes to the knowledge of non-albicans Candida species infections in patients and, opens the possibility for a better understanding and management in antifungal empirical therapy.in Mexico.
Erythrocyte antigens and antibodies in the Chilean population
Authors: Aburto A. , Zapata D. , Retamales E. , Moscoso H. , Canales C. , Escobar C. 
Published: December 2021

Abstract
Chile does not have a national registry of immunohematologic test results; there are no data on the prevalence of erythrocyte antigens and the frequency of antibodies in this population. Therefore, foreign references are used for decision-making. In this study, a standard questionnaire was used in 74 laboratories of public and private establishments. The information from tests conducted in 2015 was requested: ABO and D typing, antibody detection, antibody identification, and erythrocyte phenotype. Prevalence for the ABO-D phenotypes were obtained at the country level (D+ [94.4%] and D– [5.5%]) and differ from those recorded in the white population (85% and 15%, respectively). Positive antibody detection results were found in 0.4 and 1.3 percent of blood donors and patients, respectively; the main specificities were anti-Lea, -E, and -D in donors and anti-D, -E, and -K in patients. Inconclusive results were observed in ABO-D typing and antibody identification in donors and patients; these samples were referred to immunohematology reference laboratories for resolution. From this study, it was possible to estimate the prevalence of erythrocyte antigens and the frequency of antibodies at the national level, and this step allows us to characterize Chile’s population of blood donors and transfusion recipients and to compare the results and frequencies with other populations or countries.
Freezing and recovering rare red blood cells using liquid nitrogen
Authors: Eades B. 
Published: December 2021

Abstract
Rare red blood cells (RBCs) can be used to resolve reactivity in antibody identification or compatibility testing. Because these RBCs are usually not readily available, when identified in donors or patients, a quick and easy method for long-term storage is needed. By freezing in liquid nitrogen, injury to the RBCs is prevented, and rendering them usable for testing takes only a few washes.