bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of FOXO1-Mediated Autophagy Promotes Paclitaxel-Induced Apoptosis in MDA-MB-231 Cell Lines
Authors: Xu Kaixiang , Zhu Wanyun , Xu Anyong , Xiong Zhe , Zhao Heng , Jiao Deling , Qing Yubo , Jamal Muhammad Ameen , Wei Hong-Jiang , Zhao Hong-Ye 
Published: September 2021

Abstract
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancers and often produces resistance to paclitaxel (PTX) therapy. Autophagy plays an important cytoprotective role in PTX-induced tumor cell death and targeting autophagy is promising to improve the efficacy of tumor chemotherapy in recent years. Here, we reported that PTX induced both apoptosis and autophagy of MDA-MB-231 cells, and inhibition of autophagy enable to promote apoptotic cell death. Furthermore, we found that FOXO1 enhanced PTX-induced autophagy by a transcriptional activation pattern in MDA-MB-231 cells, which was associated with its downstream target genes ATG5, VPS34, BECN1 and MAP1LC3B. The knockdown of FOXO1 attenuated the survival of MDA-MB-231 cells under the PTX treatment. These findings will be beneficial to improve the treatment efficacy of PTX and to develop the autophagic target therapy of TNBC.
Peroxisomal Proliferator-Activated Receptor β/δ Deficiency Induces Cognitive Alterations
Authors: Espinosa-Jimenez Triana , Busquets Oriol , Cano Amanda , Verdaguer Ester , Olloquequi Jordi , Auladell Carme , Folch Jaume , Wahli Walter , Vazquez-Carrera Manel , Camins Antoni , Ettcheto Miren 
Published: September 2021

Abstract
BackgroundPPARβ/δ, the most PPAR abundant isotype in the central nervous system is involved in the modulation of microglial homeostasis and metabolism. Several studies have demonstrated that people suffering from type 2 diabetes mellitus develop cognitive decline turning insulin resistance one of the best predictors of this disturbance. Although numerous investigations have studied the role of PPARb/d in metabolism, its role in neuronal and cognitive function has been underexplored. Therefore, the aim of the study is to determine the role of PPARb/d in the neuropathological pathways involved in the development of cognitive decline and as to whether a risk factor involved in cognitive loss such as obesity modulates neuropathological markers.6-month-old male PPARβ/δ-null (PPARβ/δ-/-) and wildtype (WT) littermates with the same genetic background (C57BL/6X129/SV) and exceptionally, C57BL/6 were used. After the weaning, animals were fed either with conventional chow (CT) or with a palmitic acid-enriched diet containing 45% of fat mainly from hydrogenated coconut oil (HFD). Thus, four groups were defined: WT CT, WT HFD, PPARβ/δ-/- CT and PPARβ/δ-/- HFD and several pathological mechanisms involved in cognitive decline were analyzed.ResultsOur results confirmed that C57BL/6X129/SV showed significantly increased levels of anxiety compared to C57BL/6. Therefore, to evaluate cognitive decline, behavioral tests were dismissed, and dendritic spine quantification and other biochemical biomarkers were performed.PPARβ/δ-/- mice exhibited a decrease in dendritic spine density and synaptic markers, suggesting an alteration in cognitive function and synaptic plasticity. Likewise, our study demonstrated that the lack of PPARβ/δ receptor enhances gliosis in the hippocampus, contributing to astrocyte and microglial activation and also induced an increase in neuroinflammatory biomarkers. Additionally, alterations in the hippocampal insulin receptor pathway were found. Interestingly, while some of the disturbances caused by the lack of PPARβ/δ were not affected by feeding the HFD, others were exacerbated or required the combination of both factors.ConclusionsTaken together, these findings suggest that the loss of PPARβ/δ-/- affects neuronal and synaptic structure, contributing to cognitive dysfunction and, they also present this receptor as a possible new target for the treatment of cognitive decline.
LncRNA-AK138945 Regulates Myocardial Ischemia-Reperfusion Injury Via the miR-1-GRP94 Signaling Pathway
Authors: Wang Yanying , Shao Yingchun , Huang Jian , Li Mengmeng , Yu Qi , Gong Manyu , Yang Xuewen , Qu Yunmeng , Liu Dongping , Wang Yaqi , Li Haodong , Jiao Lei , Zhang Ying 
Published: September 2021

Abstract
Myocardial ischemia-reperfusion injury (MIRI) is one of the main causes of high mortality of cardiovascular diseases (CVD). Long non-coding RNAs (lncRNAs), as a new type of gene expression regulators, play a key role in multiple processes of CVD. In this study, myocardial apoptosis was found to be increased in both MIRI heart and hydrogen peroxide (H2O2) treated myocardial cells. Meanwhile, the level of lncRNA-AK138945 was reduced. At the molecular level, knockdown of lncRNA-AK138945 increased the expression of pro-apoptosis proteins and decreased the expression of anti-apoptosis protein. Mechanically, miR-1a-3p was verified as the target of lncRNA-AK138945, which has been previously confirmed as a target regulator of endoplasmic reticulum stress-related protein GRP94. In addition, knockdown of lncRNA-AK138945 markedly inhibited the expression of GRP94, which further aggravated the apoptosis of cardiomyocytes. Notably, FOXO3 was identified as a transcriptional regulator of lncRNA-AK138945, and overexpression of FOXO3 could promote the expression of lncRNA-AK138945. In conclusion, lncRNA-AK138945 participated in the regulation of MIRI via the miR-1-GRP94 signaling pathway.
A Safe and Effective Two-Step Tract Dilation Technique in Totally Ultrasound-Guided Percutaneous Nephrolithotomy
Authors: Dong Shao-Wei , Wu Chia-Chang , Lin Chu-Tung , Chen Kuan-Chou , Ho Chen-Hsun 
Published: September 2021

Abstract
Background To evaluate the safety and the efficacy of a radiation-free 2-step tract dilation technique in totally ultrasound-guided percutaneous nephrolithotomy (PCNL). Methods From Oct 2018 to Mar 2020, we prospectively and consecutively enrolled 18 patients with 19 kidney units with urolithiasis. The nephrostomy tract was established by the following four steps: 1) ultrasound-guided renal puncture, 2) first-stage serial dilation to 16 Fr with Amplatz dilators, 3) check and adjustment of the partially dilated tract with a ureteroscope, 4) second-stage dilation with a 24-Fr balloon dilator. Results The median age was 62.0 [IQR 11.0] years, and 11 (61.1%) were male. The median stone size was 3.3 [3.6] cm2, and stone laterality was almost equal over both sides. Successful tract establishment on the first attempt without fluoroscopy was achieved in 18 (94.7%) operations. The median tract establishment time was 10.4 [4.9] mins, and the median operation time was 67.0 [52.2] mins. The median hemoglobin drop was 1.0 [1.1] g/dL, and none required blood transfusion. Three (15.8%) developed fever. Pleural injury occurred in two (10.5%) operations (both had supracostal puncture), and one required drainage with pigtail. Stone-free status was achieved in 15 (77.8%) operations at 3 months postoperatively. Conclusions Herein we present a radiation-free 2-step tract dilation technique, which is characterized by ureteroscopic check of the partially dilated tract in between the first dilation with serial fascial dilators and the second dilation with balloon. Our data suggest that it is a safe and effective method.
Quantitative Proteomics Identifies Cannabinoid Receptor 2 as Candidate Marker of Idiopathic Pulmonary Fibrosis and Dysregulation of Endogenous Cannabinoids Pathway
Authors: du wenjing , Zhang Ting , Yang Fangyong , Tang Zhao , Li Qiuping , Gul Aman , Zhang Hu , Yi La , Wang Shiqiang , Dong Jingcheng 
Published: September 2021

Abstract
Background: Idiopathic pulmonary fibrosis (IPF) a chronic, progressive, and lung fibrosis disease of unknown etiology with less effective treatment. It is important to discover new biomarker and therapeutic target for the diagnosis and cure of IPF. Method: Differential metabolic profiles may be useful for the diagnosis of IPF and provide additional insight into the molecular mechanisms underlying IPF. Plasma samples from IPF, COPD and normal controls were investigated using liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) and these datasets were analyzed using multiple pattern performance methods. Multivariate statistical methods, pathway enrichment analysis and univariate receiver operating characteristic (ROC) curve analysis were performed. Results: OPLS-DA results showed that it exhibited significant separation between any two groups. ROC curve analyses revealed that 8 metabolites with high AUC above 0.7 between three groups in the plasma samples. Pathway analysis revealed that 3 metabolites are involved in endogenous cannabinoids/cannabinoid receptor 2 (CB2) signaling. Moreover, we found that the specific elevation of CB2 could be a signature of PF(Pulmonary Fibrosis) lung tissues in rat model. Conclusions: LC-MS-based plasma metabolomics provides a important tool to identify the potential biomarkers for IPF. Taken together, we performed quantitative chemoproteomic profiling and identified endogenous cannabinoids/CB2 as the key target of ICA in bleomycin-induced pulmonary fibrosis. Trial Registration: The study was approved by the the Ethics Committee of Huashan Hospital, Fudan University (KY2021-453)
YBX1 Mediates Alternative Splicing and Maternal mRNA Decay During Pre-Implantation Development
Authors: Deng Mingtian , Chen Baobao , Liu Zifei , Wan Yongjie , Li Dongxu , Yang Yingnan , Wang Feng 
Published: September 2021

Abstract
Background: In mammals, maternal gene products decay and zygotic genome activation (ZGA) during maternal to zygotic transition (MZT) is critical for pre-implantation. Y-box binding protein YBX1 plays vital roles in RNA stabilization and transcriptional regulation, but its roles in pre-implantation development remain to be elucidated. The objective of this study is to investigate the role and the molecular mechanisms of YBX1 during MZT.Methods: RNA-seq datasets in mice, human, bovine, and goat embryos were re-analyzed. YBX1 was knocked down by siRNA microinjection. The 8-cell stage embryos were collected for RNA-seq. The differentially expressed genes and alternative splicing (AS) events were identified using DESeq2 and rMATs, respectively. GO/KEGG/GSEA enrichment analysis was performed using clusterProfiler and enrichplot. Furthermore, 5-EU staining was performed to confirm the effect of YBX1 knockdown on transcriptional activity.Results: The expression of YBX1 was increased during MZT in goat, bovine, human, and mice. By microinjection of siRNA against YBX1, we successfully knocked down YBX1, and the embryo development was impaired in YBX1 knockdown embryos. Using RNA-seq, we identified 1623 up-regulated and 3531 down-regulated genes in the 8-cell stage YBX1 knockdown embryos. Of note, the down-regulated genes were enriched in regulation of RNA/mRNA stability and spliceosome, suggesting that YBX1 might medicate RNA stability and AS. To this end, we identified 3284 differential AS events and 1322 differentially expressed maternal mRNAs at the 8-cell stage YBX1 knockdown embryos. Meanwhile, the splicing factors and mRNA decay related showed aberrant expression. Moreover, the transcriptional activity during ZGA in goat and mice was compromised when YBX1 was knocked down.Conclusion: Our results identify that YBX1 serves an important role in maternal mRNA decay, alternative splicing, and the transcriptional activity required for early embryogenesis, which will broaden the current understanding of YBX1 functions during the stochastic reprogramming events.
Heavy ion escape from Martian wake enhanced by magnetic reconnection
Authors: Wang Lei , Huang Can , Ge Yasong , Du A. M. , Wang Rongsheng , Zhang Tielong , Fan Jinqiao 
Published: September 2021

Abstract
How ion escape from the near-Mars space is one of the biggest puzzles for understanding the atmospheric evolution of Mars. Ions in the plasma wake region continuously escape from the unmagnetized planet. Although the average ion escape rate in the wake region is relatively low, observations also have revealed the presence of events that contribute bursty and enhanced ion escape fluxes. Boundary instabilities and magnetic reconnection are suggested to be the candidate mechanisms. However, there is a lack of evaluation of ion escape caused by reconnection and comparison of the two mechanisms under a similar plasma environment. Here, we show an exciting reconnection event in the Martian wake. Two types of flux ropes are observed during the event. One was generated by reconnection, while others were produced by dayside boundary instability and convected to tail. The escape rate of oxygen ions in the reconnection region was estimated to be about 53–72% of the total tailward escape. Furthermore, the escape flux in the flux rope produced by reconnection was over twice that caused by dayside instabilities.
Combined QTL and GWAS Analysis to Identify the Growth-Related Gene in Rhopilema Esculentum with the Help of 2b-RAD Sequencing
Authors: Chen Bailing , Li Yulong , Tian Meilin , Su Hao , Sun Wei , Li Yunfeng 
Published: September 2021

Abstract
R. esculentum is a popular seafood in Asian countries and an economic marine fishery resource in China. However, the high-resolution genetic map and growth-related molecular markers still lack, hindering the process of the genetic breeding of R. esculentum. Therefore, we firstly used the 2b-RAD method to sequence 152 R. esculentum specimens, identified 9100 single nucleotide polymorphism (SNP) markers and constructed a high-resolution genetic map with a marker interval of 0.58 cM, covering 98.68% of the genome. Then, we separately detected four and three quantitative trait loci (QTLs) associated with umbrella diameter and body weight based on the linkage map, which is located on linkage group (LG) 4, 13, 14 and 15. Finally, 27 genes were found both associated with umbrella diameter and body weight of R. esculentum by genome-wide association study (GWAS), in which one gene named RE13670 containing calcium-binding EGF-like domain may play an important role in controlling the growth. This study will be beneficial for underlying the growth mechanism of R. esculentum and also provide background knowledge for guiding its genetic breeding.
Potential Mechanism of Tibetan Medicine Dali in the Treatment of Respiratory Diseases via Network Pharmacology
Authors: Fan Yuxin , Yang Yong , Wang Yunhong , Wang Jirui , Hua Lei , Zhang Xiaomei , Gao Sijia 
Published: September 2021

Abstract

Background: As a source of Tibetan medicine “Dali”, Rhododendron anthopogonoides Maxim (RAM) has good medically helpful effect on respiratory diseases. However, due to its complex components, there is still a lack of pharmacological and pharmacodynamic research on its active components. Methods: The research method of the network pharmacology was used in this study, to obtain the mechanism of RAM in the treatment of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). The active components of RAM were obtained by searching the relevant database, and the protein targets of each chemical component in RAM were screened by Traditional Chinese Medicine Database and Analysis Platform (TCMSP) and PubChem. Then, the protein targets were normalized to the corresponding gene name through UniProt. Meanwhile, genes related to ALI were searched by Online Mendelian Inheritance in Man (OMIM) database, GeneCards database, DrugBank database and DisGeNET database. The “Component-Target-Protein” network was established by Cytoscape software, and the core targets were screened by combining with STRING software. Finally, GO and KEGG enrichment analysis were carried out by David database. Results: Combined with all above results, 92 active components of RAM and 176 gene targets of ALI/ARDS were screened, including 46 common targets, which were related to biological processes such as inflammation, immune and inflammatory response, cell growth and differentiation and apoptosis. Conclusions: It can be concluded through the investigation of network pharmacology that RAM regulates a variety of physiological processes such as cell growth, differentiation, apoptosis and death by activating the Toll-like receptor signaling pathway, TNF signaling pathway, cAMP signaling pathway, MAPK signaling pathway, as well as regulating the inflammatory response of cells, the immune response of the body and promoting the inflammatory cytokines NF‐κB dependent expression and other processes to treat ALI/ARDS. This study revealed the mechanism of RAM multi-component, multi-target and multi-pathway, and provided scientific basis and research ideas for its clinical application.
The Impact of COVID-19 Vaccine on Rate of Hospitalization and Outcome of COVID-19 Infection in a Single Center in the Eastern Province of Saudi Arabia
Authors: Alkhafaji Dania M. , Argan Reem Al , Alwaheed Abrar Jamal , Alqatari Safi Ghazi , Alelq Abdulmohsen , Albaker Waleed , Bukhari Huda , Alwezzah Marwan , Alwarthan Sarah , AlSulaiman Amal Shehab , AlSulaiman Reem Shehab , Almadan Hussain Mohammed , Alhammad Ali Abdulmohsin 
Published: September 2021

Abstract
Background: Coronavirus 2019 (COVID-19) is an emerging and quickly disseminating disease that causes deleterious complications. Vaccines have the potential to improve population immunity and avoid serious disease and deaths.Methodology: A retrospective cohort study was conducted on 331 hospitalized patients with Covid 19 infections between April 2021 and July 2021 at King Fahad University Hospital. Data was collected from the medical records stored in the electronic health system of the hospital.Results: 27.7% of the participants required ICU admission, and 10.5% required mechanical ventilation. The mortality rate was around 7.23% of the infected cases. Two thirds of the study participants (64.05%) did not receive any vaccine, and it can be noted that only 16.8% had received 2 doses. The results suggest that the status of receiving a vaccine has significantly influenced the length of hospital stay, decreasing it by 19.7%. In addition, the date of receiving the vaccine was statistically significant in decreasing the incidence of ICU admission, as those who have received a vaccine for longer than 14 days needed ICU admission 82% less compared with their counterparts. The type of vaccine did not impact on any of the reported outcomes in the form of hospitalization rate, ICU admission and death.Conclusion: The majority of the study participants didn’t receive any vaccines before their admission with COVID 19, which in turn prolonged their hospital stay. This necessitates that the public require more awareness regarding the importance of receiving the vaccine.
Dysfunction of Low-Density Neutrophils in Peripheral Circulation in Patients With Sepsis
Authors: Sun Ran , Huang Jiamin , Yang Yunxi , Liu Lu , Shao Yiming , Li Linbin , Sun Bingwei 
Published: September 2021

Abstract
Background Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. Methods We studied sepsis patients admitted to the intensive care unit. WrightGiemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. Results We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. Conclusions These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future.
A Parametric Study on Dissimilar Metal Welding of Aluminum and Copper using Pulsed Fiber Laser
Authors: Trinh Lanh Ngoc , Chatterjee Suman , Lee Dongkyoung 
Published: September 2021

Abstract
Joining dissimilar metals is critically challenging due to the difference in properties of the metals themselves which leads to the formation of brittle intermetallic compounds (IMCs). Aluminum (Al) and copper (Cu) are well-known materials for electrical application as they attribute to various advantageous characteristics. In lithium-ion batteries, to obtain most of the features of the metals, combinations of these metals are highly recommended. However, with such high reflective metals and heat-sensitive characteristics in the battery, the joint of these metals needs to be processed with an advanced method. In this study, a pulsed fiber laser source that suits to process for heat-sensitive components is utilized to weld two overlap configurations of Al/Cu and Cu/Al, separately. Different ranges of laser power are designated for each welding configuration separately. Thus, the quality of the two welds is evaluated in terms of microstructure and mechanical properties. Consequently, it is found that the growth of IMCs with dendritic structure towards the Al side is observed in both cases. Moreover, the weld of Al/Cu shows a better connection strength as well as fewer imperfections than the weld of Cu/Al.
Identification and Vitro Validation of Biomarkers with Diagnostic and Prognostic for Lung Squamous Cell Carcinoma
Authors: Zhao Xiaopeng , Yu Fan , He Xu , Wang Miao , Zhang Haoran , Zhang Xuefeng , Wang Hongyan 
Published: September 2021

Abstract
Background: This study aimed to find biomarkers with diagnostic and prognostic for lung squamous cell carcinoma and to validate key biomarkers in vitroMethods：RNA sequencing was used to identify differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) between LUSC and normal tissue. Using published dataset, we also validated the result of RNA sequencing. The diagnostic and prognostic value of candidate genes were evaluated by ROC curve analysis and survival analysis, respectively. To uncover the confirm effect of MIR205HG in LUSC, we knocked down MIR205HG expression in NCI-H520 cells. Then, MTT assay and transwell assay was used to detect the effect of MIR205HG on cell proliferation and migration.Rsults: In total, 1946 DEmRNAs and 428 DElncRNAs were identified in LUSC compared to normal tissues. A total of 147 DElncRNAs-nearby target DEmRNAs pairs and 851 DElncRNA-DEmRNA co-expression pairs were obtained. Except for NEAT1, expression of the others in the The Cancer Genome Atlas (TCGA) results was consistent with our RNA sequencing, generally. ROC curve analysis displayed that the MCM2, SERPINB5, ITGB8, CASC19 and MIR205HG could predict the occurrence of LUSC. Survival analysis suggested that SERPINB5, NEAT1 and MIR205HG had potential prognostic value for LUSC. Knockdown of MIR205HG inhibits cell proliferation and migration in NCI-H520 cell. In addition, knockdown of MIR205HG significantly reduced the expression of ITGB8.Conclusions: This data may contribute to uncover the pathogenesis of LUSC and provide new and accurate therapeutic targets for LUSC.