bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-dependent Lupus Ruminococcus blautia gnavus strain induction of zonulin-mediated intestinal permeability and autoimmunity
Authors: Deng Jing , Azzouz Doua F. , Ferstler Nicole , Silverman Gregg J. 
Published: July 2021

AbstractImbalances in the gut microbiome are suspected as contributors to the pathogenesis of Systemic Lupus Erythematosus, and our studies and others have documented that patients with active Lupus nephritis have expansions of the obligate anaerobe, Ruminococcus blautia gnavus (RG). To investigate whether the RG strains in Lupus patients have in vivo pathogenic properties, we colonized C57BL/6 mice with individual RG strains from healthy adults or those from Lupus patients. These strains had a similar capacity for murine intestinal colonization, in antibiotic-preconditioned specific-pathogen-free, as well as germ-free adults, and their neonatally colonized litters. Lupus-derived RG strains induced high levels of intestinal permeability that was significantly greater in female than male mice, whereas the RG species-type strain (ATCC29149/VPI C7-1) from a healthy donor had little or no effects. Lupus RG strain-induced functional alterations were associated dysregulated occluden transcript production in the ileal wall as well as raised serum levels of zonulin, a regulator of tight junction formation between cells that form the gut barrier. Notably, the level of Lupus RG-induced intestinal permeability was significantly correlated with serum IgG anti RG cell-wall lipoglycan antibodies, and to anti-native DNA autoantibodies that are a biomarker for SLE. Strikingly, gut permeability was completely reversed by oral treatment with larazotide acetate, an octapeptide that is a specific molecular antagonist of zonulin. Taken together, these studies document a molecular pathway by which RG strains from Lupus patients induce a leaky gut and autoimmunity that have been implicated in the pathogenesis of flares of clinical Lupus disease.
Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: Application to direct RNA sequencing on nanopores
Authors: Vo Jenny , Mulroney Logan , Quick-Cleveland Jen , Jain Miten , Akeson Mark , Ares Manuel 
Published: July 2021

ABSTRACTUnderstanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) that act on nucleic acids for capturing and sequencing RNA. We tested two 3’ nucleotidyl transferases,Saccharomyces cerevisiaepoly(A) polymerase andSchizosaccharomyces pombeCid1, for the ability to add base and sugar modified rNTPs to free RNA 3’ ends, eventually focusing on Cid1. Although unable to polymerize ΨTP or 1meΨTP, Cid1 can use 5meUTP and 4thioUTP. Surprisingly, Cid1 can use inosine triphosphate to add poly(I) to the 3’ ends of a wide variety of RNA molecules. Most poly(A) mRNAs efficiently acquire a uniform tract of about 50 inosine residues from Cid1, whereas non-poly(A) RNAs acquire longer, more heterogeneous tails. Here we test these activities for use in direct RNA sequencing on nanopores, and find that Cid1-mediated poly(I)-tailing permits detection and quantification of both mRNAs and non-poly(A) RNAs simultaneously, as well as enabling the analysis of nascent RNAs associated with RNA polymerase II. Poly(I) produces a different current trace than poly(A), enabling recognition of native RNA 3’ end sequence lost by in vitro poly(A) addition. Addition of poly(I) by Cid1 offers a broadly useful alternative to poly(A) capture for direct RNA sequencing on nanopores.
DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome-wide
Authors: Altemose Nicolas , Maslan Annie , Smith Owen K. , Sundararajan Kousik , Brown Rachel R. , Detweiler Angela M. , Neff Norma , Miga Karen H. , Straight Aaron F. , Streets Aaron 
Published: July 2021

AbstractMolecular studies of genome regulation often rely on the ability to map where specific proteins interact with genomic DNA. Existing techniques for mapping protein-DNA interactions genome-wide rely on DNA amplification methods followed by sequencing with short reads, which dissociates joint binding information at neighboring sites, removes endogenous DNA methylation information, and precludes the ability to reliably map interactions in repetitive regions of the genome. To address these limitations, we created a new protein-DNA mapping method, calledDirectedMethylation withLong-readsequencing (DiMeLo-seq), which methylates DNA near each target protein’s DNA binding sitein situ, then leverages the ability to distinguish methylated and unmethylated bases on long, native DNA molecules using long-read, single-molecule sequencing technologies. We demonstrate the optimization and utility of this method by mapping the interaction sites of a variety of different proteins and histone modifications across the human genome, achieving a single-molecule binding site resolution of less than 200 bp. Furthermore, we mapped the positions of the centromeric histone H3 variant CENP-A in repetitive regions that are unmappable with short reads, while simultaneously analyzing endogenous CpG methylation and joint binding events on single molecules. DiMeLo-seq is a versatile method that can provide multimodal and truly genome-wide information for investigating protein-DNA interactions.
Corporate social responsibility actions and organizational legitimacy at the peak of COVID-19: a sentiment analysis
Authors: Giacomini Davide , Martini Mattia , Sancino Alessandro , Zola Paola , Cavenago Dario 
Published: July 2021

Purpose
This paper aims to analyse stakeholder sentiment about the corporate social responsibility (CSR) actions implemented by Italian companies between February 20, 2020 and April 20, 2020, which was the first peak in the outbreak of the COVID-19 health emergency in Italy.

Design/methodology/approach
Using sentiment analysis, the impact of COVID-19 on CSR actions is analysed through reactions to the news published on Twitter by a sample of Italian news agencies.

Findings
The analysis indicates that the actions most appreciated are those that are more radical, e.g. where the company has converted part of its production to make goods that are useful in dealing with the COVID-19 emergency. The study identifies a new category of actions definable as “crisis-shaped CSR.”

Practical implications
This is one of the first studies concerning the effects of the pandemic on both CSR actions and organizational legitimacy.

Originality/value
This work explains which strategic approach to CSR is the most effective in supporting corporate reputation in times of crisis, this study identified which of the CSR initiatives adopted by companies in Italy were more effective in stimulating positive interactions and sentiment among the general public.
Service encounters with virtual agents: an examination of perceived humanness as a source of customer satisfaction
Authors: Söderlund Magnus , Oikarinen Eeva-Liisa 
Published: July 2021

Purpose
Firms have begun to introduce virtual agents (VAs) in service encounters, both in online and offline environments. Such VAs typically resemble human frontline employees in several ways (e.g. the VAs may have a gender and a name), which indicates the presence of an assumption by VA designers – and by firms that employ them – that VA humanness is a positively charged characteristic. This study aims to address this assumption by examining antecedents to perceived humanness in terms of attribution of agency, emotionality and morality, and the impact of perceived humanness on customer satisfaction.

Design/methodology/approach
A questionnaire was distributed online to participants who had been interacting with existing VAs, and they were asked to focus on one of them for this study. The questionnaire comprised measures of antecedents to perceived humanness of VAs, perceived humanness per se and customer satisfaction. A structural equation modeling approach was used to assess associations between the variables.

Findings
Attributions of agency, emotionality and morality to VAs contributed positively to the perceived humanness of the VAs, and perceived humanness was positively associated with customer satisfaction.

Research limitations/implications
Additional humanness capabilities should be explored in further research.

Practical implications
Firms using VAs in service encounters should make attempts to maximize perceived VA humanness, and this study shows that it may be beneficial if such attempts comprise signals that VAs have agency, emotionality and morality.

Originality/value
By examining VAs in terms of a set of fundamental human capabilities, the present study contributes to existing research on human–VA service encounters, which to date has focused on more superficial VA characteristics (such as if the VA has a face and gender).
Migrant health as a human right amidst COVID-19: a culture-centered approach
Authors: Dutta Mohan J. 
Published: July 2021

Purpose
The purpose of this manuscript is to examine the negotiations of health among low-wage migrant workers in Singapore amidst the COVID-19 outbreaks in dormitories housing them. In doing so, the manuscript attends to the ways in which human rights are constituted amidst labor and communicative rights, constituting the backdrop against which the pandemic outbreaks take place and the pandemic response is negotiated.

Design/methodology/approach
The study is part of a long-term culture-centered ethnography conducted with low-wage migrant workers in Singapore, seeking to build communicative infrastructures for rights-based advocacy and interventions.

Findings
The findings articulate the ways in which the outbreaks in dormitories housing low-wage migrant workers are constituted amidst structural contexts of organizing migrant work in Singapore. These structural contexts of extreme neoliberalism work catalyze capitalist accumulation through the exploitation of low-wage migrant workers. The poor living conditions that constitute the outbreak are situated in relationship to the absence of labor and communicative rights in Singapore. The absence of communicative rights and dignity to livelihood constitutes the context within which the COVID-19 outbreak emerges and the ways in which it is negotiated among low-wage migrant workers in Singapore.

Originality/value
This manuscript foregrounds the interplays of labor and communicative rights in the context of the health experiences of low-wage migrant workers amidst the pandemic. Even as COVID-19 has made visible the deeply unequal societies we inhabit, the manuscript suggests the relevance of turning to communicative rights as the basis for addressing these inequalities. It contributes to the extant literature on the culture-centered approach by depicting the ways in which a pandemic as a health crisis exacerbates the challenges to health and well-being among precarious workers.
Analysing the complexities of sustainable wood supply chain in the Amazon: a systems thinking approach
Authors: Elias Arun A. , Donadelli Flavia , Paiva Ely L. , Bacic Araujo Paulo Philipe 
Published: July 2021

PurposeThe overall objective of this study is to holistically analyse the complexities involved in the adoption of sustainable wood supply chain in the Amazon and to develop strategic interventions to improve the system.Design/methodology/approachThis study uses the systems thinking and modelling framework that included problem structuring followed by the development of a systems model. Tumbira and Santa Helena do Inglês, two Amazonian communities, were used as a case. Data were collected by staying inside the Amazon, observing the community members and interacting with them during their activities, including logging inside the forest.FindingsA behaviour over time (BOT) graph developed as a part of problem structuring showed that deforestation of the Amazon is still increasing, despite the creation of protected areas and NGO activities in the region. Developing a sustainable wood supply chain is considered as one of the approaches for sustainable forestry in the Amazon, but its adoption is slow. The systems model captured the underlying structure of this system and explained this counterintuitive behaviour using eleven interacting feedback loops.Research limitations/implicationsThis study is confined to two Amazonian communities and recognises the limitations of generalisations.Practical implicationsThis paper illustrates the development of three strategic interventions to improve the adoption of sustainable wood supply chain in the Amazon.Originality/valueThis study highlights the real issues faced by real communities living in the Amazon for adopting sustainable supply chain management (SSCM) practices. It contributes to the literature on sustainable wood supply chain by systemically analysing the challenges in its adoption, not sufficiently discussed in the literature.
Process quality management and operational performance: exploring the role of learning and development orientation
Authors: Sahoo Saumyaranjan 
Published: July 2021

PurposeThe paper aims to assess the influence of process quality management (PQM) practices on a firm's operational performance (OP) and investigate the mediating role of a firm's learning and development orientation (LDO) in the PQM–OP relationship. The paper also assesses the extent to which the proposed mediation is moderated by senior management support (SMS).Design/methodology/approachThis paper draws on the sociotechnical system theory to carry out a survey study of 278 functional managers from various manufacturing firms in India through a postal mail survey approach. The response rate has been improved by frequent telephone follow-ups. The collected data was analyzed for mediation of LDO and moderated mediation of SMS in the proposed framework using Hayes's PROCESS Macro in statistical package for social sciences.FindingsThe research findings show that both PQM and LDO lead to better performance and should be encouraged. LDO was found to partially mediate the effect of PQM on the firm's OP. The results also show that an enhanced learning capability of an organization can significantly influence improvement in OP through a stronger support of senior management.Research limitations/implicationsThe current study explored the mediating role of LDO in the relationship between PQM and OP, while providing a future research opportunity for an empirical review of the mediating effect of LDO between the effects of just-in-time, maintenance and process benchmarking activities on OP.Practical implicationsTo transform PQM activities into a competitive advantage, firms need to develop learning capabilities. Senior managers in the manufacturing industry should strongly focus upon creating a learning-centered work culture to improve performance and productivity.Originality/valueThe study provides insights into the type of people-related activities that manufacturing firms should undertake to enhance their OP. Few studies in the extant literature have used SMS as a moderator to improve LDO's impact on business operations. The study aims at bridging this gap.
Parenting advantages of emerging market multinationals (EMNCs) in luxury fashion retailing
Authors: Bai Huifeng , He Weijing , Shi Jin , McColl Julie , Moore Christopher 
Published: July 2021

PurposeThis empirical research, adopting an international retailing perspective, aims to examine the parenting advantages offered by emerging market multinationals (EMNCs) in luxury fashion retail sector.Design/methodology/approachThe researchers adopted a qualitative case study, and the qualitative data were collected through ten semi-structured interviews with senior managers.FindingsIt is a win–win situation for the EMNCs as parent groups of Western luxury fashion brands, as the EMNCs can access critical assets including advanced brand management expertise, retailing know-how, and the services skills needed for higher income consumers. Meanwhile, the subsidiary brands benefit from a high degree of autonomy, intra-group resource utilisation, a competitive brand portfolio and most importantly economies of scales in the value chain, particularly in production. The perceived risks of EMNCs ownership include potentially restricted autonomy and the uncertainty over corporate development activities in the future, as well as the risks of diluting brand image caused by the inconsistency between country of origin and country of ownership.Research limitations/implicationsVery few EMNCs have moved into luxury fashion retailing to date, which means that the sampling frame was small. The findings were generated from China, which is perceived to be of considerable psychic distance in terms of culture and policies compared to other emerging markets that have been heavily influenced by colonialism.Practical implicationsThis paper suggests that practitioners, particularly EMNCs, support their subsidiary luxury fashion brands through parenting advantages and develop their own high-end fashion brands through internationalisation.Originality/valueThis empirical study contributes to the current international retailing literature by offering in depth insights of parenting advantages offered by EMNCs in luxury fashion retailing. It also enriches the EMNC literature, which has mainly adopted an international business scope, by extending this understanding into luxury fashion retailing.
Structural equation modeling of residents' attitudes and tourism development: a SET based - study of the Kashmir region in India
Authors: Bhat Aasif Ali , Majumdar Kakali 
Published: July 2021

PurposeThe present study tries to develop a model that assesses the factors that determine support for tourism development by residents of the Kashmir region.Design/methodology/approachPrimary data have been collected (n = 650) from the residents of the top five tourist destinations through a pre-tested questionnaire by a multistage convenient sampling method. A model has been drafted and tested through the technique of structural equation modeling by applying the social exchange theory as a theoretical framework.FindingsThe results revealed that residents who perceived more benefits were more expected to support tourism development, and residents who perceive more costs were less expected to support tourism development, thus supporting the social exchange theory.Originality/valueThe results of this study are extremely useful for the local government and tourism institutions in the future planning of tourism development and also fill the vast gap in the tourism literature with a theoretical base.
Design and optimization of actuator for multi-joint soft rehabilitation glove
Authors: Wang Xinjie , Cheng Yan , Zheng Huadong , Li Yihao , Wang Caidong 
Published: July 2021

Purpose
Currently, rehabilitation medical care is expensive, requires a large number of rehabilitation therapist and which can only limit in the fixed location. In addition, there is a lack of research on the structure optimization and theoretical analysis of soft actuators for hand rehabilitation. In view of the problems above, this paper aims to propose a cheap, portable, wearable soft multiple joints rehabilitation glove.

Design/methodology/approach
First, this paper determined the hyperelastic constitutive model by material tensile test. Second, the soft actuator’s internal longitudinal section shape was optimized through the comparison of three diverse chamber structures. Meanwhile, the motion model of the soft actuator is established by the finite element model analysis method. Then, this paper established the constitutive model of the soft actuator according to the torque equilibrium equation and analyzed the relationship between the soft actuator’s bending angle and the input air pressure. This paper has verified that the theoretical model is correct through the soft actuator bending test. Finally, rehabilitation gloves were manufactured according to the model and the rehabilitation performance and grasping ability of gloves were verified through experiments.

Findings
The optimization results show that the internal semicircular cavity has better performance. Then, the actuator performance is better after adding the external arc structure and optimizing the physical dimension. The experimental results show that the trajectory of the actuator conforms to the mathematical model and rehabilitation gloves can meet the needs of rehabilitation treatment.

Practical implications
Rehabilitation gloves made of actuators can help patients with hand dysfunction in daily rehabilitation training. Then, it can also assist patients with some fine and complicated hand movements.

Originality/value
This paper proposes a new type of soft rehabilitation glove, which is composed of new soft actuators and adapting pieces. The new actuator is small enough to be fitted to the knuckle of the glove to move each joint of the finger.
Challenges faced by older people in a district of Uttar Pradesh: a qualitative study
Authors: Patel Avanish Bhai 
Published: July 2021

Purpose
The steady rise in aged population has brought many challenges such as social, economic and health care that confront of the older people in their later life. The purpose of this study is to understand the nature of challenges among the older people and to assess the role of social security programmes for the welfare of the older people. The qualitative descriptive research has been applied in this paper.

Design/methodology/approach
The qualitative descriptive research has been applied in this paper. The study was conducted in a sample of 220 elderly living in Lucknow, Uttar Pradesh, using purposive sampling. The study was based on interviews. The order of questions and samples depended on the information flow during the interviews. The purposes of using the qualitative descriptive research in the present study are to satisfy the researcher’s curiosity and desire for better understanding to discuss the challenges faced by older people (these challenges are social vulnerability, poor economic conditions, poor health and no familiarity with government programmes), to understand the practicability of the study in extensive way and to explain why any phenomenon occurs or why older people face problems in later life.

Findings
The first finding demonstrates that the different challenges among older people such as social, economic and health challenges are affecting their way of life and sense of well-being and are fracturing their social bonds from the family and society. The second finding indicates that only 46.3% older people are benefitted from government pension programmes schemes. While the numbers of older people are unknown from other government welfare programmes such as health programmes, concession for older people and maintenance and welfare of parent and senior citizen act are not able to work properly due to lack of awareness and lack of proper communication between older people and government bodies.

Originality/value
This is an original work of the author. The research work is based on primary data that examine the nature of challenges such as health, economic and social challenges faced by older people in later life and impact of these problems on the well-being of older people.