bluuebunny/crossref_metadata_2025_split huggingface dataset:
BldD-based bimolecular fluorescence complementation for in vivo detection of the second messenger cyclic di-GMP
Authors: Halte Manuel , Wörmann Mirka E. , Bogisch Maxim , Erhardt Marc , Tschowri Natalia 
Published: August 2021

AbstractThe widespread bacterial second messenger bis-(3’-5’)-cyclic diguanosine monophosphate (c-di-GMP) is an important regulator of biofilm formation, virulence and cell differentiation. C-di-GMP-specific biosensors that allow detection and visualization of c-di-GMP levels in living cells are key to our understanding of how c-di-GMP fluctuations drive cellular responses. Here, we describe a novel c-di-GMP biosensor, CensYBL, that is based on c-di-GMP-induced dimerization of the effector protein BldD from Streptomyces resulting in bimolecular fluorescence complementation of split-YPet fusion proteins. As a proof-of-principle, we demonstrate that CensYBL is functional in detecting fluctuations in intracellular c-di-GMP levels in the Gram-negative model bacteria Escherichia coli and Salmonella enterica serovar Typhimurium. Using deletion mutants of c-di-GMP diguanylate cyclases and phosphodiesterases, we show that c-di-GMP dependent dimerization of CBldD-YPet results in fluorescence complementation reflecting intracellular c-di-GMP levels. Overall, we demonstrate that the CensYBL biosensor is a user-friendly and versatile tool that allows to investigate c-di-GMP variations using single-cell and population-wide experimental set-ups.ImportanceThe second messenger c-di-GMP controls various bacterial functions including development of resistant biofilm communities and transition into dormant spores. In vivo detection of c-di-GMP levels is therefore crucial for a better understanding of how intracellular c-di-GMP levels induce changes of bacterial physiology. Here, we describe the design of a novel c-di-GMP biosensor and demonstrate its effective application in investigating fluctuations in intracellular c-di-GMP levels in Escherichia coli and Salmonella enterica serovar Typhimurium on a population-based and single-cell level.
The entomopathogenic nematodeSteinernema hermaphroditumis a self-fertilizing hermaphrodite and a genetically tractable system for the study of parasitic and mutualistic symbiosis
Authors: Cao Mengyi , Schwartz Hillel T. , Tan Chieh-Hsiang , Sternberg Paul W. 
Published: August 2021

AbstractEntomopathogenic nematodes, includingHeterorhabditisandSteinernema, are parasitic to insects and contain mutualistically symbiotic bacteria in their intestines (PhotorhabdusandXenorhabdus,respectively) and therefore offer opportunities to study both mutualistic and parasitic symbiosis. The establishment of genetic tools in entomopathogenic nematodes has been impeded by limited genetic tractability, inconsistent growthin vitro, variable cryopreservation, and low mating efficiency. We obtained the recently describedSteinernema hermaphroditumstrain CS34 and optimized itsin vitrogrowth, with a rapid generation time on a lawn of its native symbiotic bacteriaXenorhabdus griffiniae. We developed a simple and efficient cryopreservation method. Previously,S. hermaphroditumisolated from insect hosts was described as first-generation hermaphroditic and second-generation gonochoristic. We discovered that CS34, when grownin vitro,produced consecutive generations of autonomously reproducing hermaphrodites accompanied by rare males. We performed mutagenesis screens inS. hermaphroditumthat produced mutant lines with visible and heritable phenotypes. Genetic analysis of the mutants demonstrated that this species reproduces by self-fertilization rather than parthenogenesis and that its sex is determined chromosomally. Genetic mapping has thus far identified markers on the X chromosome and three of four autosomes. We report thatS. hermaphroditumCS34 is the first consistently hermaphroditic entomopathogenic nematode and is suitable for genetic model development to study naturally occurring mutualistic symbiosis and insect parasitism.
The transcriptomic signature of obligate parthenogenesis
Authors: Xu Sen , Huynh Trung , Snyman Marelize 
Published: August 2021

AbstractInvestigating the origin of parthenogenesis through interspecific hybridization can provide insight into how meiosis may be altered by genetic incompatibilities, which is fundamental for our understanding of the formation of reproductive barriers. Yet the genetic mechanisms giving rise to obligate parthenogenesis in eukaryotes remain understudied. In the microcrustacean Daphnia pulex species complex, obligately parthenogenetic (OP) isolates emerged as backcrosses of two cyclically parthenogenetic (CP) parental species, D. pulex and D. pulicaria, two closely related but ecologically distinct species. We examine the genome-wide expression in OP females at the early resting egg production stage, a life-history stage distinguishing OP and CP reproductive strategies, in comparison to CP females of the same stage from the two parental species. Our analyses of the expression data reveal that misregulated genes (underdominant and overdominant genes) are abundant in OP isolates, suggesting widespread regulatory incompatibilities between the parental species. More importantly, underdominant genes (i.e., genes with expression lower than both parentals) in the OP isolates are enriched in meiosis and cell-cycle pathways, indicating an important role of underdominance in the origin of obligate parthenogenesis. Furthermore, metabolic and biosynthesis pathways enriched with overdominant genes (i.e., expression higher than both parentals) are another genomic signature of OP isolates.
Development of the first antibody targeting vitellogenin using the Palinurus elephas mRNA molecular pathway
Authors: Cannea Faustina B , Follesa Cristina , Porcu Cristina , Rossino Rossano , Olianas Alessandra , Rescigno Antonio , Padiglia Alessandra 
Published: August 2021

ABSTRACTVitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar oestrogen pollutants and affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.
Impact of bacterial microcompartment-dependent ethanolamine and propanediol metabolism on Listeria monocytogenes interactions with Caco-2 cells
Authors: Zeng Zhe , Wijnands Lucas M. , Boeren Sjef , Smid Eddy J. , Notebaart Richard A. , Abee Tjakko 
Published: August 2021

AbstractBacterial microcompartment (BMC) dependent ethanolamine (eut) and propanediol utilization (pdu) has recently been shown to stimulate anaerobic growth of Listeria monocytogenes. This metabolic repertoire conceivably contributes to the competitive fitness of L. monocytogenes in the human gastrointestinal (GI) tract, where these compounds become available following phospholipid degradation and mucus-derived rhamnose metabolism by commensal microbiota. Previous transcriptomics and mutant studies of eut and pdu L. monocytogenes suggested a possible role of eut and pdu BMC metabolism in transmission in foods and pathogenicity, but data on a potential role of L. monocytogenes interaction with human cells is currently absent. First, we ask which cellular systems are expressed in the activation of eut and pdu BMC metabolism and the extent to which these systems are conserved between the states. We find common and unique systems related to metabolic shifts, stress and virulence factors. Next, we hypothesize that these common and unique activated cellular systems contribute to a role in the interaction of L. monocytogenes interaction with human cells. We present evidence that metabolically primed L. monocytogenes with active eut and pdu BMCs, as confirmed by metabolic analysis, transmission electron microscopy and proteomics, show significantly enhanced translocation efficacy compared to non-induced cells in a trans-well assay using Caco-2 cells, while adhesion and invasion capacity was similar. Taken together, our results provide insights into the possible key cellular players that drive translocation efficacy upon eut and pdu BMC activation.
Single-cell chromatin and transcriptome dynamics of Synovial Fibroblasts transitioning from homeostasis to pathology in modelled TNF-driven arthritis
Authors: Armaka Marietta , Konstantopoulos Dimitris , Tzaferis Christos , Lavigne Matthieu D , Sakkou Maria , Liakos Anastasios , Sfikakis Petros P , Dimopoulos Meletios A , Fousteri Maria , Kollias George 
Published: August 2021

AbstractSynovial fibroblasts (SFs) are specialized cells of the synovium that provide nutrients and lubricants for the maintenance of proper function of diarthrodial joints. Chronic TNF signals are known to trigger activation of SFs and orchestration of arthritic pathology via proinflammatory effector functions, secretion of cartilage degrading proteases and promotion of osteolysis. We performed single-cell (sc) profiling of SF’s transcriptome by RNA-sequencing (scRNA-seq) and of chromatin accessibility by scATAC-seq in normal mouse SFs and SFs derived from early and advanced TNF-driven arthritic disease. We describe here distinct subsets of SFs in the homeostatic synovium, serving diverse functions such as chondro- and osteogenesis, tissue repair and immune regulation. Strikingly, development of spontaneous arthritis by transgenic TNF overexpression primes the emergence of distinct pathology-associated SF subtypes. We reveal 7 constitutive and 2 disease-specific SF subtypes. The latter emerge in the early stage, expand in late disease and are localized in areas at the interface between the invasive pannus and the articular bone. The associated transcription profiles are characterized by enhanced inflammatory responses, promigratory behaviour, neovascularization and collagen metabolic processes. Temporal reconstruction of transcriptomic events indicated which specific sublining cells may function as progenitors at the root of trajectories leading to intermediate subpopulations and culminating to a destructive lining inflammatory identity. Integrated analysis of chromatin accessibility and transcription changes revealed key transcription factors such as Bach and Runx1 to drive arthritogenesis. Parallel analysis of human arthritic SF data showed highly conserved core regulatory and transcriptional programs between the two species. Therefore, our study dissects the dynamic SF landscape in TNF-mediated arthritis and sets the stage for future investigations that might address the functions of specific SF subpopulations to understand joint pathophysiology and combat chronic inflammatory and destructive arthritic diseases.
Understanding citizen engagement and concerns about the COVID-19 pandemic in China: a thematic analysis of government social media
Authors: Luo Lijuan , Duan Siqi , Shang Shanshan , Lyu Wenfei 
Published: August 2021

PurposeIn crises such as the coronavirus disease 2019 (COVID-19) pandemic, governments need to act in time to lead citizens toward rational reactions and disclose information effectively to the public. This paper aims to understand the content published by the government and identify how citizen engagement relates to content type and emotional valence.Design/methodology/approachThe grounded theory approach was adopted and nine types of content posted by the government were observed. The data were obtained from “People's Daily”, an official Sina Weibo account representing the voice of the Chinese government, from January 3 to June 22 in 2020.FindingsThe analysis shows that information related to emotional support and social mobilization were the most reposted, while those mentioning immoral and illegal incidents were the most liked and commented. Also, it was found that positive posts tend to attract more likes, yet with fewer reposts than neutral posts.Originality/valueThe authors adopted thematic analysis and focused on the impact of post content and valence on user participation behavior. This study expands the existing literature. The government can improve crises management capability by learning about citizen engagement behaviors on social media.
What drives customers towards mobile shopping? An integrative technology continuance theory perspective
Authors: Jain Nikunj Kumar , Kaul Dimple , Sanyal Priyavrat 
Published: August 2021

PurposeExisting studies examine the effect of mobile service quality (M-S-QUAL) on shoppers' intent to continue mobile shopping using various theoretical lenses to understand its underlying factors. However, there is limited research focusing on combining theories to integrate the understanding of the factors that contribute to continuance intention of customers towards mobile shopping. This study integrated M-S-QUAL, the technology adoption model (TAM) and technology continuance theory (TCT) to test our proposed conceptual model of mobile shopping useDesign/methodology/approachEmpirical data from a survey of 193 respondents who frequently make purchases using mobile and its applications were analysed using partial least squares based on structural equation modelling (PLS-SEM).FindingsThis study found that M-S-QUAL and perceived usefulness (PU) positively influence shopping satisfaction and CI. PU and innovativeness, positively affect shopping satisfaction and CI. The study also found support for the mediating effect of satisfaction between M-S-QUAL and PU with CI.Research limitations/implicationsThis study contributes to TCT by incorporating pre-purchase and post-purchase dimensions for mobile shopping through applications. Pre-purchase variables include technology-related and innovativeness-related features. In contrast, post-purchase variables include the service quality of forward and reverse logistics.Practical implicationsThe service providers should focus on technology and innovativeness to increase the continuing intent of shoppers through mobile applications.Originality/valueThis study validates fulfilment, responsiveness and contact as key determinants of service quality in mobile shopping. It also identifies the practical implications to the managers for improving service quality, innovativeness and the PU of mobile shopping to retain customers.
Whether and how managers' negotiable fate belief and knowledge search affect enterprise innovation
Authors: Zhang Shanliang , Guo Chen , Wang Yongwei 
Published: August 2021

PurposeThe purpose of this paper is to explore the mechanism and boundary condition of the effect of managers' negotiable fate belief (NFB) on enterprise incremental and radical innovation based on social cognition theory and social network theory.Design/methodology/approachThe authors used Mplus and SPSS (Statistical Product and Service Solutions) to test the proposed model with data from 278 enterprises that have established national or municipal enterprise technology centers. In this study, questionnaires were collected through commission and field investigation.FindingsThe results indicate that managers' NFB has a significant positive impact on local and cross-border knowledge search and on the incremental and radical innovation of enterprises. Knowledge search is positively correlated with enterprise innovation and can play a mediating role between the managers' NFB and enterprise innovation. Network capability can positively moderate the relationship between managers' NFB and cross-border knowledge search but has no significant impact on the relationship between managers' NFB and local knowledge search.Originality/valueAlthough there have been many studies of managers' cognition, little is known about how managers' NFB influences enterprise behavior and enterprise innovation. This study incorporates managers' NFB and knowledge search into the research model and examines the moderating effect of network capability between managers' NFB and knowledge search from an interactive perspective. By verifying the research model, this study offers original views on whether and how managers' NFB and knowledge search affect enterprise innovation.
Embedding ensemble learning into simulation-based optimisation: a learning-based optimisation approach for construction planning
Authors: Feng Kailun , Chen Shiwei , Lu Weizhuo , Wang Shuo , Yang Bin , Sun Chengshuang , Wang Yaowu 
Published: August 2021

PurposeSimulation-based optimisation (SO) is a popular optimisation approach for building and civil engineering construction planning. However, in the framework of SO, the simulation is continuously invoked during the optimisation trajectory, which increases the computational loads to levels unrealistic for timely construction decisions. Modification on the optimisation settings such as reducing searching ability is a popular method to address this challenge, but the quality measurement of the obtained optimal decisions, also termed as optimisation quality, is also reduced by this setting. Therefore, this study aims to develop an optimisation approach for construction planning that reduces the high computational loads of SO and provides reliable optimisation quality simultaneously.Design/methodology/approachThis study proposes the optimisation approach by modifying the SO framework through establishing an embedded connection between simulation and optimisation technologies. This approach reduces the computational loads and ensures the optimisation quality associated with the conventional SO approach by accurately learning the knowledge from construction simulations using embedded ensemble learning algorithms, which automatically provides efficient and reliable fitness evaluations for optimisation iterations.FindingsA large-scale project application shows that the proposed approach was able to reduce computational loads of SO by approximately 90%. Meanwhile, the proposed approach outperformed SO in terms of optimisation quality when the optimisation has limited searching ability.Originality/valueThe core contribution of this research is to provide an innovative method that improves efficiency and ensures effectiveness, simultaneously, of the well-known SO approach in construction applications. The proposed method is an alternative approach to SO that can run on standard computing platforms and support nearly real-time construction on-site decision-making.
An integrative framework of innovative work behavior for employees in SMEs linking knowledge sharing, functional flexibility and psychological empowerment
Authors: Yasir Muhammad , Majid Abdul , Yousaf Zahid , Nassani Abdelmohsen A. , Haffar Mohamed 
Published: August 2021

PurposeThis study aims to propose a model based on knowledge sharing (KS) to explore that how functional flexibility (FF) and psychological empowerment (PE) plays a mediating role between KS and innovative work behavior (IWB) among employees of small and medium enterprises (SMEs).Design/methodology/approachCross-sectional plan was employed to investigate the hypothesized model. Survey instrument was used to collect data from 769 employees of SMEs through self-administered questionnaires. Furthermore, correlation and structural equation modeling approach was used for the analysis of collected data.FindingsThe findings revealed that KS has a significant positive relationship with IWB. Second, FF and PE fully mediate between KS and IWB.Originality/valueThis study offers empirical evidence how to flourish a mechanism of IWB among employees in SMEs. Furthermore, this study highlighted some facts that should be meaningful options for human resource managers to understand the nexus of KS, FF and PE for enhancing the IWB among workers in SMEs. Moreover, the study in hand provided significant implications for management by focusing on KS as a fundamental predictor for IWB via FF and PE.
Consequences of employee personality in the hospitality context: a systematic review and meta-analysis
Authors: Doan Tin , Kanjanakan Pattamol , Zhu Dan , Kim Peter B. 
Published: August 2021

Purpose
Personality provides a critical perspective for human resource managers on differences between employees. This study aims to systematically and meta-analytically synthesize the consequences of employee personality in the hospitality context.

Design/methodology/approach
After an extensive literature search, 105 empirical studies on the consequences of the big five personality factors (BFF; agreeableness, conscientiousness, extraversion, neuroticism and openness) along with proactive personality (PP) in the hospitality context were included for a systematic review and meta-analysis.

Findings
The review highlighted a steady increase in the number of studies on hospitality employee personality. Job satisfaction and organizational citizenship behavior were identified as the most significant consequences for employee personality in the hospitality context. Five dimensions of personality traits varied in their consequences and differed from PP.

Research limitations/implications
This study provides insightful implications and suggestions for future studies in terms of methodological approaches, research topics and dimensions of employee personality that will extend the theoretical framework of individual differences.

Originality/value
To the best of the authors’ knowledge, this study represents the first attempt to systematically investigate the consequences of employee personality in the hospitality context. The results reveal discrepancies in the relations between the dimensions of BFF and PP with a variety of consequences. These results offer research directions for hospitality scholars investigating employee personality.
Psychological resilience and its relationship to life stress among Al-Bayda University students
Authors: Al-dhuraibi Abdullah Mohammed , Ali Al-Abyadh Mohammed Hasan 
Published: August 2021

Purpose
The phenomenon of stress is a problem for a large number of interested people and researchers, and it has even become a global phenomenon in recent times. This is due to the effect of this phenomenon on the individual's performance, efficiency and satisfaction with the work he performs. This paper aims to know the level of psychological resilience, life stress and the relationship between them among a sample of Al-Bayda University students in the health-care context in Republic of Yemen.

Design/methodology/approach
To achieve the study objectives, the researchers applied the psychological resilience scale, which consisted of (40) items and prepared the psychological stress scale that consisted of (41) items.

Findings
The findings of the study indicated that the level of psychological resilience of Al-Bayda University students is high. The findings also indicated that there are statistically significant differences in psychological resilience depending on the gender variable in favor of males and that there are no statistically indicative significant differences according to the variables of specialization, academic level and marital status. The findings showed a high prevalence of life stresses among the members of the study sample and indicated that the sources of stress among the study sample individuals were arranged as follows: academic stress, future stress, social stress, family stress and economic stress.

Originality/value
The findings indicated significant differences among the study sample in the stress of life according to the variable of gender in favor of males and the presence of an inverse significant relationship between the level of psychological resilience and life stress among the study sample members.