bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactomic analysis reveals a new homeostatic role for the HIV restriction factor TRIM5α in mitophagy
Authors: Saha Bhaskar , Salemi Michelle , Williams Geneva L , Paffett Michael L , Phinney Brett , Mandell Michael A 
Published: August 2021

ABSTRACTThe protein TRIM5α has multiple roles in anti-retroviral defense, but the mechanisms underlying TRIM5α action are unclear. Here, we used an APEX2-based proteomics approach to identify TRIM5α-interacting proteins. Analysis of the TRIM5α interactome found proteins participating in a wide variety of cellular functions including regulating antiviral signaling pathways. We used this data set to uncover a novel role for TRIM5α in mitophagy, an autophagy-based mode of mitochondrial quality control that is compromised in multiple human diseases. Mitochondrial damage triggered the relocalization of TRIM5α to ER-mitochondria contact sites where TRIM5α colocalized with markers of autophagy initiation and autophagosome biogenesis. Furthermore, we found that TRIM5α knockout attenuated both Parkin-dependent and Parkin-independent mitophagy by preventing the recruitment of autophagy regulators FIP200 and ATG13 to unhealthy mitochondria. Finally, TRIM5α knockout cells showed reduced mitochondrial function under basal conditions and were more susceptible to uncontrolled immune activation and cell death in response to mitochondrial damage than were wild type cells. Taken together, our studies have identified a homeostatic role for a protein previously recognized exclusively for its antiviral actions.
Machine learning enables discovery of DNA-carbon nanotube sensors for serotonin
Authors: Kelich Payam , Jeong Sanghwa , Navarro Nicole , Adams Jaquesta , Sun Xiaoqi , Zhao Huanhuan , Landry Markita P. , Vuković Lela 
Published: August 2021

AbstractDNA-wrapped single walled carbon nanotube (SWNT) conjugates have remarkable optical properties leading to their use in biosensing and imaging applications. A critical limitation in the development of DNA-SWNT sensors is the current inability to predict unique DNA sequences that confer a strong analyte-specific optical response to these sensors. Here, near-infrared (nIR) fluorescence response datasets for ~100 DNA-SWNT conjugates, narrowed down by a selective evolution protocol starting from a pool of ~1010 unique DNA-SWNT candidates, are used to train machine learning (ML) models to predict new unique DNA sequences with strong optical response to neurotransmitter serotonin. First, classifier models based on convolutional neural networks (CNN) are trained on sequence features to classify DNA ligands as either high response or low response to serotonin. Second, support vector machine (SVM) regression models are trained to predict relative optical response values for DNA sequences. Finally, we demonstrate with validation experiments that integrating the predictions of ensembles of the highest quality CNN classifiers and SVM regression models leads to the best predictions of both high and low response sequences. With our ML approaches, we discovered five new DNA-SWNT sensors with higher fluorescence intensity response to serotonin than obtained previously. Overall, the explored ML approaches introduce an important new tool to predict useful DNA sequences, which can be used for discovery of new DNA-based sensors and nanobiotechnologies.
SARS-CoV-2 hijacks p38β/MAPK11 to promote virus replication
Authors: Higgins Christina A. , Nilsson-Payant Benjamin E. , Kurland Andrew P. , Ye Chengjin , Yaron Tomer , Johnson Jared L. , Bonaventure Boris , Adhikary Prithy , Golynker Ilona , Panis Maryline , Danziger Oded , Rosenberg Brad R. , Cantley Lewis C. , Martinez-Sobrido Luis , tenOever Benjamin R. , Johnson Jeffrey R. 
Published: August 2021

AbstractSARS-CoV-2, the causative agent of the COVID-19 pandemic, drastically modifies infected cells in an effort to optimize virus replication. Included is the activation of the host p38 mitogen-activated protein kinase (MAPK) pathway, which plays a major role in inflammation and is a central driver of COVID-19 clinical presentations. Inhibition of p38/MAPK activity in SARS-CoV-2-infected cells reduces both cytokine production and viral replication. Here, we combined genetic screening with quantitative phosphoproteomics to better understand interactions between the p38/MAPK pathway and SARS-CoV-2. We found that several components of the p38/MAPK pathway impacted SARS-CoV-2 replication and that p38β is a critical host factor for virus replication, and it prevents activation of the type-I interferon pathway. Quantitative phosphoproteomics uncovered several SARS-CoV-2 nucleocapsid phosphorylation sites near the N-terminus that were sensitive to p38 inhibition. Similar to p38β depletion, mutation of these nucleocapsid residues was associated with reduced virus replication and increased activation of type-I interferon signaling. Taken together, this study reveals a unique proviral function for p38β that is not shared with p38α and supports exploring p38β inhibitor development as a strategy towards developing a new class of COVID-19 therapies.ImportanceSARS-CoV-2 is the causative agent of the COVID-19 pandemic that has claimed millions of lives since its emergence in 2019. SARS-CoV-2 infection of human cells requires the activity of several cellular pathways for successful replication. One such pathway, the p38 mitogen-activated protein kinase (MAPK) pathway, is required for virus replication and disease pathogenesis. Here, we applied systems biology approaches to understand how MAPK pathways benefit SARS-CoV-2 replication to inform the development of novel COVID-19 drug therapies.
Cannabidiol activates neuronal Kv7 channels
Authors: Niday Zachary , Heckman Laurel , Jo Sooyeon , Zhang Han-Xiong Bear , Fujita Akie , Shim Jaehoon , Pandey Roshan , Jandal Hoor Al , Jayakar Selwyn , Smith Jennifer A. , Woolf Clifford J. , Bean Bruce P. 
Published: August 2021

AbstractCannabidiol (CBD), a chemical found in the Cannabis sativa plant, is a clinically effective antiepileptic drug whose mechanism of action is unknown. Using a fluorescence-based thallium flux assay, we performed a large-scale screen and found enhancement of flux through heterologously-expressed human Kv7.2/7.3 channels by CBD. Using patch clamp recordings, we found that CBD at low concentrations activates Kv7.2/7.3 channels at subthreshold voltages, with 100 nM CBD producing a doubling of current at -50 mV. CBD shifted the voltage-dependence of channels in the hyperpolarizing direction, producing a shift in the midpoint of activation by ∼-14 mV at 300 nM. CBD also effectively enhanced native M-current in both mouse superior cervical ganglion neurons and rat hippocampal neurons. The potent enhancement of Kv2/7.3 channels by CBD seems likely to contribute to its effectiveness as an antiepileptic drug by reducing neuronal hyperexcitability.
Accounting for the “transcendent self”: spirituality, narcissism, testimony and gift
Authors: Achilli Giulia , Busco Cristiano , Giovannoni Elena 
Published: August 2021

PurposeThe paper explores the process of construction of the “accountable self”, particularly as this process engages with the spirituality of the self. This study examines the “space of accountability” within which the accountable self constructs itself as such and investigates how different accounts of the self are drawn upon in the making of this space, both defining and transcending it.Design/methodology/approachThe paper relies upon archival material concerning accounting and accountability practices about the project for building the altar of St. Ignatius in the Church of Gesù, Rome, Italy (1691–1706). This study examines calculative and narrative accounts about the project from the perspective of the superintendent, who was the sole person accountable for the building works.FindingsWhereas calculative accounts enabled the self to account for actions within the specific space of accountability of the project, narrative accounts opened up this space, providing for a testimony of actions and a gift of accountability towards future indefinite others. This process was prompted by the spirituality of the self and the narcissistic gratification of fulfilling this spirituality.Originality/valueThe paper adds to the literature on the accountable self and to theological perspectives into accountability. This study suggests exploring how different accounts of the self engage with each other through testimony, gift, narcissism and spirituality in the construction of the accountable self, providing for a “transcendent” space of accountability. This research also adds to studies on narrative accounts by showing that they are drawn upon alongside calculative accounts in the construction of the transcendent, accountable self.
What drives persuasion to choose healthy and ecological food at hotel buffets: message, receiver or sender?
Authors: Volgger Michael , Cozzio Claudia , Taplin Ross 
Published: August 2021

PurposeThe effectiveness of a message to persuade tourists to consume healthier and more ecological food may be influenced by (1) the message content, (2) the message sender and (3) the receiver of the message. This study analyses effective persuasion of tourists by simultaneously investigating how these three characteristics influence tourists' healthy and ecological food choices at hotel buffets.Design/methodology/approachA randomised 4 × 2 between-subject experimental design, replicated in two hotels, collected perceptions of 329 participants on four persuasive messages aimed at increasing food consumption in a realistic and natural setting at hotel buffets. The eight treatments included four persuasive messages (placebo appeal, health appeal, environmental appeal, local origin appeal), each with and without endorsement (i.e. with/without a specific sender).FindingsMessage content, in particular health and local provenance-related messages, and the characteristics of the receiver (tourists' attitudes and habits) influence food choices on holiday while the sender (endorsement) was found to be less influential. This implies that appropriate messages can persuade tourists to eat in a considerate manner from hotel buffets, especially for tourists with favourable attitudes and everyday behaviours.Originality/valueThis paper contributes to theory and practice in persuasive communication to encourage healthier and more ecological choices in hedonistic consumption. Specifically, this paper is the first to present an integrated empirical analysis on the simultaneous influence of (1) message content, (2) presence of an identifiable sender and (3) receiver characteristics, on healthy and ecological eating behaviour of tourists.
Integrated management system: methodology for maturity assessment in food industries
Authors: Santos Ângela Regina Souza , Melo Renata Maciel de , Clemente Thárcylla Rebecca Negreiros , Machado Santos Simone 
Published: August 2021

PurposeA model to evaluate the maturity of the integration of three management systems in food industries, under the aspects of environment, quality and food safety is proposed.Design/methodology/approachThe model is composed of maturity levels, allowing the understanding of the integration of the systems mentioned and their stage of evolution. The work was based on a bibliographic review of the topics: ISO standards, Integrated Management Systems (IMS) and already existing methods for maturity assessment. The referred model is composed of elements related to (1) audit dynamics, (2) changes in behavior, (3) the organizational structure, (4) the participation of senior management and (5) the interface with the cycle of continuous improvement.FindingsThe model is easily applicable and composed of five maturity levels, allowing the understanding of the stage of system evolution: (1) absence of maturity, (2) introductory level, (3) intermediate level, (4) toward excellence and (5) excellence.Research limitations/implicationsThe results of the model may help managers in the development of actions for the improvement of their integrated management (drivers for IMS) aiming at process control, the focus on the client and the integration of people and processes. Based on the drivers, the managers will be able to make plans characterized by orientations, techniques and tools applied in the several organizational processes. Using the proposed model, managers can enhance the desired results with efficiency and effectiveness.Originality/valueThe existing literature presents limited results on the integration of certified systems for the analysis of the maturity stages in manufacturing industries. Therefore, this study extends results from other studies on IMS, associating them to an organizational maturity analysis in food industries, resulting in a proposal of maturity model. This study also proposes the integration of ISO 14001, ISO 9001 and ISO 22000 to indicate the maturity stage of manufacturing industries in the food sector, supporting the strategic management process of organizations of any size. The model adopts several aspects of management and control to provide a holistic view on the integrated management of organizations. Finally, the proposed model considers several aspects of management and control for the food industries in the developing countries, providing a holistic view of integrated management.
Benchmarking of Singapore maritime cluster: the role of cluster facilitators
Authors: Osman Shahrin , Sundarakani Balan , Reve Torger 
Published: August 2021

PurposeThis article analyses the role of cluster facilitators in the Singapore maritime cluster. Singapore has been recognised for its pro-business policies and its ability to attract international shipping companies to set up the ship ownership headquarters and ship management activities in Singapore.Design/methodology/approachThe research is an empirical investigation on the approach for industrial cluster development of the Singapore maritime cluster, using the case study research methodology. The case study approach leverages on multiple sources of evidence from deep interviews (of 24 Singaporean firms and 13 Norwegian firms) related observations, documentation and archival records. As a means of contributing to the cluster renewal process, Singapore as the country embarks on the next stage of maritime cluster development, a benchmarking against the Norwegian Innovation Cluster has been incorporated.FindingsThe research findings reveals that Singapore is lacking in innovation activities that entails multi-firms collaborations and collaboration between multi-firms and research institutions. The existence of cluster organisation to facilitate collaborations between firms in the cluster and between firms in the cluster with research institutions is another contributing factor that are not institutionalised in the Singapore maritime cluster.Research limitations/implicationsThough the research is grounded primarily on the international business theory, particularly from firm- and country-specific advantages of location decisions, the economic geography theory and cluster theory also complement the theoretical grounding.Practical implicationsThe findings derived from this research aim to facilitate policy makers, maritime leaders and practitioners to develop effective courses of action in current and future maritime industry development.Originality/valueThe research provides value to maritime industry stakeholders, maritime leaders and policy makers in their firm positioning strategy. Thus, the research adds values to the maritime industry with similar country perspectives and firm values for developing policies.
Manufacturing-oriented silicon steel coil lengthwise cutting stock problem with useable leftover
Authors: Li Fengjie , Chen Yan , Hu Xiaochun 
Published: August 2021

PurposeThis paper propose an algorithm for the multiple silicon steel coils multiperiod two-dimensional lengthwise cutting stock problem (m2DLCSP), so as to minimize the total cost of materials and production.Design/methodology/approachThe authors propose a sequential leftovers utilization correction (SLUC) algorithm for the m2DLCSP. The algorithm primarily considers three optimization strategies. First, it considers usable leftovers to simplify the cutting process and improve material utilization. The total quantity and types of leftovers should be limited in order to avoid leftover overstock. Second, it uses a splice method of items to improve the generated cutting plan. Third, it takes into account operational restrictions in the cutting operations. Operational restrictions include imposing maximum and minimum lengths on the cutting patterns, and the limitation of cutting knives at the slitting machines.FindingsSeveral sets of benchmark with real-world and randomly generated instances are provided to evaluate the algorithm. Compared with literature algorithm and current procedure applied in enterprises, the computational results indicate that proposed algorithm can effectively reduce the total cost, and the computation time is reasonable for practical use.Social implicationsThis algorithm can effectively reduce the total cost.Originality/valueThe proposed method can effectively applied to solve the m2DLCSP and improve the economic efficiency of enterprises.
Labour market inclusion of Afghan refugees in Pakistan through Bourdieu's theory of capital
Authors: Ali Faiza , Hennekam Sophie , Syed Jawad , Ahmed Adnan , Mubashar Rabbia 
Published: August 2021

PurposeThis article examines the labour market inclusion of documented and undocumented Afghan refugees in Pakistan using and extending Bourdieu's theory of capital.Design/methodology/approachThe authors draw on 22 semi-structured in-depth interviews with both documented and undocumented Afghan refugees in Pakistan.FindingsThe findings show the low capital endowments of refugees. Their economic capital is shaped by low levels of financial resources, and emotional capital is shaped by their psychological distress and traumata and identity capital takes the form of negative perceptions about them. Their low capital endowments are further reduced through different forms of symbolic violence, such as ambiguous and short-term government policies, bribery and abuse by the police as well as unfair treatment by employers. However, refugees do mobilise their capital endowments to enhance their labour market position. The authors identified resilience as emotional capital, their strategic development of who they are as identity capital as well as social and cultural capital in the form of ethnic and linguistic similarities with locals in finding ways to improve their inclusion in the labour market.Originality/valueThe authors provide insights in the dynamics that lead to and sustain the exclusion and inequalities faced by Afghan refugees in Pakistan.
Leadership for sustainability: fostering organizational learning to achieve radical innovations
Authors: Domínguez-Escrig Emilio , Mallén-Broch Francisco Fermín 
Published: August 2021

PurposeWhile concerns about the social and environmental impact that result from business activity continue to grow, a stream of research consolidates to understand the mechanisms that can favor more sustainable companies. The present study tries to expand the knowledge of the antecedents of radical innovation by analyzing the effects of alternative and understudied constructs. Grounded on stewardship and organizational learning theories, this paper analyzes how leaders that are concerned with sustainability and the social impact of their companies may boost this type of innovation by facilitating an organizational context that promotes experimentation, dialog, participative decision-making, risk-taking and interaction with the external environment.Design/methodology/approachThrough structural equation modeling, the study provides empirical evidence of the positive effect of stewardship leader behavior on radical innovation, using organizational learning capability as an explanatory variable.FindingsResults suggest that organizational learning capability fully mediates the relationship between stewardship leader behavior and radical innovation.Research limitations/implicationsThis research focuses on a sample frame of Spanish companies with recognized excellence in human resources management.Practical implicationsIn the context of a growing interest in sustainable development, and concern for the consequences of economic and business activities, this study highlights the role played by stewardship leader behavior to foster radical innovation and organizational learning capability which, in turn, represent essential tools to compete in a globalized and turbulent context.Originality/valueTo the authors’ knowledge, this is the first empirical study that analyzes the mediating effect of organizational learning capability in the relationship between stewardship leader behavior and radical innovation. This paper contributes to the understanding of how stewardship leader behavior affects radical innovation and the key role played by organizational learning capability.
Application of SPD-RBF method of lines for solving nonlinear advection–diffusion–reaction equation with variable coefficients
Authors: Mesgarani Hamid , Kermani Mahya , Abbaszadeh Mostafa 
Published: August 2021

PurposeThe purpose of this study is to use the method of lines to solve the two-dimensional nonlinear advection–diffusion–reaction equation with variable coefficients.Design/methodology/approachThe strictly positive definite radial basis functions collocation method together with the decomposition of the interpolation matrix is used to turn the problem into a system of nonlinear first-order differential equations. Then a numerical solution of this system is computed by changing in the classical fourth-order Runge–Kutta method as well.FindingsSeveral test problems are provided to confirm the validity and efficiently of the proposed method.Originality/valueFor the first time, some famous examples are solved by using the proposed high-order technique.
Drug price control in Malaysia: a stakeholder analysis
Authors: Ashraf Amirul , Ong Siew Chin 
Published: August 2021

PurposePharmaceutical pricing has always been a contentious issue around the world. Recently, the Government of Malaysia has proposed for drug price control. This proposal has received backlash from pharmaceutical industry. Therefore in this study, the authors would like to examine the position of various stakeholders on this issue.Design/methodology/approachThe authors used Policy Maker software to identify and evaluate the position of the stakeholders, their interest and their power. Next, the authors contextualize the factors that might contribute to the success or failure of the policy using Kingdon's multiple stream analysis.FindingsThe authors found that the drug price control regulation received mixed reactions from the stakeholders, with the public sector mainly supporting the proposal whereas the private sectors opposing it. The findings indicate that the drug price control proposal have a high chance of success due to strong political will from both the government and the opposition and also strong support from the public and the consumer groups.Practical implications1. The drug price control proposal received strong political will and strong public support. Thus, it has a high chance of success. 2. The government should be careful in managing concentrated powerful groups. The government should strategically engage with the stakeholders to move them to more positive attitude. 3. The government should avoid backtracking on policies as this will portray critical weakness to other stakeholders.Social implicationsThe public sector provides strong support for the regulation. However, this support must be maintained and remain as their top priority. Their opinions are important in shaping health policies. The public's feedback will provide transparency and accountability in the policy-making process.Originality/valueThe findings indicate that the drug price control proposal have a high chance of success due to strong political will from both the government and the opposition and also strong support from the public and the consumer groups.