bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hUCMSCs Exerts Excellent Anti-inflammation Effect which is Different from TCZ IL-6 Antagonist by Inducing High IL-6 Secretion
Authors: Lo Huei-Yu , Cheng Shun-Ping , Huang Jing-Long , Chang Kuo-Ting , Chang Yu-Lung , Huang Chien-Hsun , Chang Chia-Jen , Chiu Chien-Hua , Chen-Yang Yui Whei , Chan Chin-Kan 
Published: February 2021

Abstract
BACKGROUND The biologic and cellular IL-6-related therapies have been used to treat the autoimmune diseases (AID), which prompting us to furtherly explore the IL-6 role in human umbilical cord mesenchymal stem cells (hUCMSCs) therapy.MATERIALS & METHODS Peripheral blood mononuclear cells (PBMCs) were responder co-cultured with hUCMSCs or exogenous IL-6. PBMC suppression assay was used to analyze the anti-inflammatory effects by using the MTT assay. The IL-6 concentration in supernatant was measured using ELISA. The correlation between anti-inflammation effect of hUCMSCs and IL-6 levels, and the relevant roles of IL-6, IL-6 mRNA expression was analyzed using the MetaCore functional network constructed from gene microarray data. The location of IL-6 and IL-6 receptor (IL-6R) expression was furtherly evaluated.RESULTS Initially, hUCMSCs did not exert any inhibitory effect on PBMCs, however, a potent inhibitory effect on PBMCs was observed and the IL-6 concentration reached about 1000 ng/mL after 72 hours. Exogenous 1000 ng/mL IL-6 could inhibit PBMCs inflammations but less than that of hUCMSCs. The hUCMSCs exerts excellent anti-inflammation effect by inducing higher IL-6 level which is different from TCZ IL-6 antagonistCONCLUSIONS High concentration IL-6 cytokine secretion plays an important role in the anti-inflammation effect of hUCMSCs cell therapy.
Characteristics of menstrual cycle disorder and saliva metabolomics of young women in high-temperature environment
Authors: Wei Fan Meng , An Hong Gai , Fan Jun Li , Chen Wei Xue , Li Chao , Chen Jun Jia , Ma Qiang , Yang Feng Dan , Wang Jing 
Published: February 2021

Abstract
Objective: High-temperature environments induce menstrual disorders, which can seriously damage women's reproductive health and workability. The regulation mechanism underlying the induction of these disorders at high temperature is yet to be elucidated. Saliva is an information-rich biological fluid that can reflect systemic diseases. Blood metabolites can enter the saliva through extracellular, intracellular, or paracellular pathways. Therefore, saliva metabolites may provide a window for the early detection of diseases. Methods: Here, we investigated the characteristics of menstrual cycle disorders and saliva metabolomics. Women from high and normal temperature areas of China were selected and divided into two groups—high-temperature (H group) and control (C group). A questionnaire survey was conducted in summer (July) to investigate the incidence rate of menstrual disorders, characteristics of the disorders, and factors influencing the risk of these disorders in different regions. Metabolomics was applied to analyze the characteristics of the salivary metabolites and neurotransmitters in the two groups of women with menstrual disorders.Results: The incidence rate of menstrual disorders was significantly higher in the H group than in the C group (P < 0.05). High-temperature environment, anxiety, pressure, and sleep quality were identified as critical factors associated with menstrual disorders. Non-targeted saliva metabolomics identified 64 significantly different metabolites, which mainly enriched in metabolic pathways such as carbohydrate metabolism, membrane transport, digestive system, and nucleotide metabolism(P < 0.05). Targeted metabolomics of neurotransmitters revealed increased expression of histamine (HA) and glutamine and decreased expression of 5-hydroxyindole acetic acid (5-HIAA) (P < 0.05).Conclusions: The incidence rate of menstrual disorder was increased in the H group, which is significantly related to factors such as anxiety and pressure. Changes in metabolites and neurotransmitters indicated that besides mood, endocrine regulation mechanism, inflammatory reactions that affect the formation of the follicular, cell membrane might contribute to the development of menstrual disorders.
A SiGe-Source Doping-Less Double-Gate Tunnel FET: Design and Analysis Based on Charge Plasma Technique with Enhanced Performance
Authors: Mishra Varun , Verma Yogesh Kumar , Gupta Santosh Kumar , Rathi Vikas 
Published: February 2021

Abstract
In this article, a distinctive charge plasma (CP) technique is employed to design two doping-less dual gate tunnel field effect transistors (DL-DG-TFETs) with Si0.5Ge0.5 and Si as source material. The CP methodology resolves the issues of random doping fluctuation and doping activation. The analog and RF performance has been investigated for both the proposed devices i.e. Si0.5Ge0.5 source DL-DG-TFET and Si-source DL-DG-TFET in terms of drive current, transconductance, cut-off frequency. In addition, the linearity and distortion analysis has been carried out for both the proposed devices with respect to higher order transconductance (gm2 and gm3), VIP2, IMD3, and HD2. The Si0.5Ge0.5 source DL-DG-TFET has better performance characteristics and reliability in compare to Si-source DL-DG-TFET owing to low energy bandgap material and higher mobility. The switching ratio obtained for Si0.5Ge0.5 source DL-DG-TFET is order of 5×1014 that makes it a suitable contender for low power applications.
RGL2 Drives the Metastatic Progression of Colorectal Cancer via Preventing the Protein Degradation of β-catenin and KRAS
Authors: Sun Meng-Shun , Yuan Lan-Ting , Kuei Chia-Hao , Lin Hui-Yu , Chen Yen-Lin , Chiu Hui-Wen , Lin Yuan-Feng 
Published: February 2021

Abstract

Background: Colorectal cancer (CRC) is a common cancer and results in high mortality worldwide, owing to cancer progression, e.g., metastasis. However, the molecular mechanism underlying the metastatic evolution of CRC remains largely unknown. In this study, we investigated the clinical relevance and oncogenic function of RGL2 in CRC.Methods: Cellular migration ability was assessed by transwell cultivation. The metastatic potential was estimated by lung colony-forming assay. DNA-binding activity of transcription factors was determined by luciferase-based reporter assay. RGL2 expression was analyzed by immunohistochemistry in human CRC specimens. RGL2 mRNA expression from the Cancer Genome Atlas (TCGA) CRC database was compared between the primary tumors and normal tissues.Results: The upregulation of Ral Guanine Nucleotide Dissociation Stimulator Like 2 (RGL2) was commonly detected in primary tumors compared with normal tissues and is significantly associated with a poorer prognosis in CRC patients. Moreover, RGL2 expression appeared to positively correlate with the metastatic potential of CRC cells. Whereas RGL2 knockdown dramatically suppressed the metastatic potential of CRC cells in vitro and in vivo, RGL2 overexpression in poorly metastatic CRC cells and reconstitution in RGL2-silenced CRC cells enhanced and rescued the cellular metastatic ability, respectively. Computational simulation using the Gene Set Enrichment Analysis program and cell-based assays demonstrated that RGL2 expression was causally associated with the activity of the Wnt/β-catenin signaling axis and KRAS, as well as the progression of epithelial-mesenchymal transition (EMT) in the detected CRC cells. Importantly, RGL2 upregulation was capable of preventing the protein degradation of β-catenin and KRAS in CRC cells. Conclusion: RGL2 has metastasis-promoting effects via stabilizing β-catenin and KRAS in CRC cells and prognostic significance in CRC patients. These findings suggest that RGL2 acts as a driver to promote the metastatic progression of CRC and serves as a poor prognostic biomarker in CRC patients.
Quadruple-editing of MAPK and PI3K pathways effectively blocks the progression of KRAS-mutated colorectal cancer cells
Authors: Wang Zaozao , Kang Bin , Gao Qianqian , Huang Lei , Fan Yingcong , Yu Jianhong , Di Jiabo , Jiang Beihai , Gao Feng , Wang Dan , Sun Haixi , Gu Ying , Li Jian , Su Xiangqian 
Published: February 2021

Abstract
Background

Mutated KRAS promotes the activation of mitogen-activated protein kinase (MAPK) pathway and the progression of colorectal cancer (CRC) cells. Aberrant activation of phosphatidylinositol 3‑kinase (PI3K) pathway strongly attenuates the efficacy of MAPK suppression in KRAS-mutated CRC cells. The development of a novel strategy targeting dual-pathway is therefore highly essential for the therapy of KRAS-mutated CRC cells.
Methods

In this study, a quadruple-depleting system for KRAS, MEK1, PIK3CA, and mammalian target of rapamycin (MTOR) genes based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/SaCas9 was developed. Serotype 5 of adenovirus (ADV5) was used as packaging virus for systemic delivery of the CRISPR system. To enhance infection efficiency and specificity of ADV5 to CRC cells and reduce its non-specific tissue tropism, two engineered proteins, an adaptor and a protector were synthesized and formed an ADV-protein complex (APC) when delivered the quadruple-editing system intravenously in vivo.
Results

The quadruple-editing significantly inhibited MAPK and PI3K pathways in CRC cells with oncogenic mutations of KRAS and PIK3CA or with KRAS mutation and compensated PI3K activation. Compared with MEK and PI3K/MTOR inhibitors, the quadruple-editing induced more significant survival inhibition on primary CRC cells with oncogenic mutations of KRAS and PIK3CA. The adaptor protein which specifically targeting epithelial cell adhesion molecule (EpCAM) could dramatically enhance infection efficiency of ADV5 to CRC cells. and the protector protein could significantly reduce the off-targeting tropisms in a variety of organs. Moreover, the quadruple-editing intravenously delivered by APC significantly blocked dual-pathway and tumor growth, without influencing normal tissues in cell- and patient-derived xenograft models of KRAS-mutated CRC cells.
Conclusions

APC-delivered quadruple-editing of MAPK and PI3K pathways effectively and specifically blocked the progression of KRAS-mutated CRC cells.
Endocardial lead extraction with transoesophageal echocardiography guidance for cardiovascular implantable electronic device (CIED) related infections: four cases report
Authors: Han Qiaoyu , Feng Yi , Ju Hui , Jiang Yan , Ze Feng , Li Xuebin , Jiang Luyang 
Published: February 2021

Abstract
Background

With the expanding use of cardiac implantable electronic device (CIEDs) in older populations with more complicated conditions have bring about higher rates of CIED infections. The recommended treatment of which involves the complete removal of all hardware, followed by antibiotic therapy and re-implant. Application of transoesophageal echocardiography (TEE) helps improving efficacy and safety by capacitating the operators to perform the procedure, guiding them to better plans and to rapidly recognize and manage relative complications.
Case presentation

: We report four cases of CIED infections from single-centre, who were extracted endocardial leads with intra-op TEE monitoring and guidance. Three of them had three fatal complications as pulmonary embolism, pericardial tamponade and tricuspid trauma. The other one was found a massive vegetation detected by TEE not by pre-op TTE, which avoided intra-op embolism.
Conclusions

Continuously intra-op TEE should be one of the routine monitoring methods in these high-risk endocardial leads extraction procedures.
E-learning-based medical education during COVID19 pandemic From Medical students' View Points
Authors: Khojasteh Laleh , Karimian Zahra , Nasiri Elham , Sharifzadeh Sedigheh , Farrokhi Majid Reza 
Published: February 2021

Abstract
Background

Most universities around the world have replaced e-learning with face-to-face methods after Covid-19 Pandemic. The present study evaluates the students' viewpoints about the dimensions of e-learning during the COVID-19 pandemic.
Methods

This is a survey study aimed at investigating e-learning experiences of 550 students of Shiraz University of Medical Sciences(SUMS) during the time Coronavirus pandemic. To data collection, a research-made questionnaire was employed, and the data were analyzed using one sample T-test, Independent T-test, ANOVA, and MANOVA.
Results

The results demonstrated that the students had satisfactory e-learning experiences and the average dimensions of e-learning were: Technological facilities(M = 4.29 ± 1.13),Technical support (M = 4.00 ± 1.08), e-Content quality(3.84 ± 1.05), Asynchronous education (M = 3.73 ± 1.20), Teacher-learner interaction(3.63 ± 1.32) and Synchronous education(M = 3.59 ± 1.31)Respectively. Most important factor was the interaction and feedback between teacher and students. Students' views on the components of e-learning were related to their age, access to electronic devices, and their computer skills significantly.
Conclusion

It seems that e-Learning can be a relatively good alternative to face-to-face training, but the teacher-student interaction is the main element in the greater effectiveness of this method.
Large Faraday effect and structural properties of heavily Tb3+-doped borogermanate glasses: a potential precursor of magneto-optical fibers
Authors: Franco Douglas , Ledemi Yannick , Correr Wagner , Morency Steeve , Afonso Conrado , Messaddeq Sandra Helena , Messaddeq Younès , Nalin Marcelo 
Published: February 2021

Abstract
New glass compositions containing high concentrations of Tb3+ ions were developed aiming at the production of magneto-optical (MO) fibers. This work reports on the structural and MO properties of a new glass composition based on (100-x)(41GeO2-25B2O3-4Al2O3-10Na2O-20BaO) – xTb4O7. Morphological analysis (HR-TEM) of the sample with the highest concentration of Tb3+ ions confirmed the homogeneous distribution of Tb3+ ions and the absence of nanoclusters. All the samples presented excellent thermal stability against crystallization (ΔT > 100 oC). An optical fiber was manufactured by a fiber drawing process. The UV-Vis spectra of the glasses showed Tb3+ electronic transitions and optical windows varying from 0.4 to 1.6 µm. The magneto-optical properties and the paramagnetic behaviors of the glasses were investigated using Faraday rotation experiments. The Verdet constant (VB) values were calculated at 500, 650, 880, 1050, 1330, and 1550 nm. The maximum VB values obtained at 650 and 1550 nm for the glass with x = 18 mol% were − 128 and − 17.6 rad T− 1 m− 1, respectively. The VB values at 500 and 1550 nm for the optical fiber containing 8 mol% of Tb4O7 were − 110.2 and − 9.5 rad T− 1 m− 1, respectively, while the optical loss at around 880 nm was 6.4 dB m− 1.
The Effects of Bracing on Pelvic Parameters in Adolescent Idiopathic Scoliosis
Authors: Li Kepeng , Miao Jun , Zhang Jingan , Wang Xijie , Han Ye 
Published: February 2021

Abstract
Background: This study aims to evaluate the effects of bracing on the Cobb’s angle (CA) and spinopelvic parameters in adolescent idiopathic scoliosis (AIS) patients. Methods: A total of 51 AIS patients who received bracing treatment between January 2018 and August 2019 were retrospectively analyzed. The pro-bracing and in-bracing radiographs were analyzed with regard to the spinopelvic parameters. The CA, pelvic coronal obliquity angle (PCOA), thoracolumbar kyphosis (TK), lumbar lordosis (LL), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), sagittal vertical angle (SVA) and coronal vertical angle (CVA) were measured. Results: The mean age at the initiation of bracing was 13.6 ± 1.5 years. The mean pro-bracing CA was 24.0° ± 6.3°. There were no statistically significant differences between pro-bracing and in-bracing measurements of SVA and CVA. However, there were statistically significant differences between the pro-bracing and in-bracing measurements of the CA, PCOA, TLK, LL, PT and SS. A significant correlation was observed between PT variation and TLK variation in the sagittal plane. In the coronal plane, the PCOA variation was correlated to pro-bracing PCOA. Conclusion: Bracing effects of AIS can be extended to the pelvis. The pelvis should retro-rotate correspondingly to TLK hypokyphosis on sagittal plane, whereas in coronal plane, pelvic obliquity was improved independently.
Influence of Comorbidities on Functional Outcomes in Patients With Surgically Treated Fragility Hip Fractures: a Retrospective Cohort Study
Authors: Yoon Soo Hoon , Kim Bo Ryun , Lee Sang Yoon , Beom Jaewon , Choi Jun Hwan , Lim Jae-Young 
Published: February 2021

Abstract
Background: The incidence and number of fragility hip fractures are gradually increasing, resulting in a wide consumption of medical resources. Various factors affecting functional recovery in patients with fragility hip fractures are known, and comorbid diseases are one of them. The purpose of this study is to determine the effect of comorbidities on functional outcomes in patients surgically treated for fragility hip fractures, thereby contributing to the efficient distribution of medical resources.Methods: This was a retrospective cohort study performed in the three tertiary rehabilitation facilities. A total of 211 patients (50 men and 161 women; average age 81.6±6.7 years) who had undergone surgery for fragility hip fractures were followed up from immediately after transfer to the Department of Rehabilitation Medicine to 6 months postoperatively. Comorbidities referred to a summary of the following conditions: hypertension, diabetes mellitus, chronic liver disease, dementia, cerebrovascular accident, and osteoporosis. Functional outcomes included Koval’s grade, functional ambulatory category (FAC), functional independence measure (FIM)-locomotion, modified Rivermead mobility index, Berg balance scale (BBS), 4-meter walking speed test, the Korean version of the Mini-Mental State Examination, Geriatric Depression Scale (GDS), EuroQol five-dimension (EQ-5D) questionnaire, the Korean version of the Modified Barthel Index, the Korean version of the instrumental activities of daily living (K-IADL), and Korean version of fatigue, resistance, ambulation, illnesses, and loss of weight scale (K-FRAIL). For all tests, each patient was assessed immediately after transfer and 6 months post-surgery.Results: Multivariate linear regression analyses adjusted for age, sex, the initial variable of the functional outcomes, and comorbidities revealed that dementia had a significant negative impact on Koval’s grade and K-FRAIL 6 months postoperatively. Diabetes mellitus had a significant negative impact on the FAC, GDS, EQ-5D, K-IADL, and K-FRAIL 6 months postoperatively. Patients with osteoporosis showed a significant negative outcome of FIM-locomotion 6 months postoperatively. A cerebrovascular accident revealed a significant negative impact on the BBS 6 months postoperatively. In addition, hypertension led to significantly less favorable outcomes of the K-FRAIL 6 months postoperatively. Conclusions: This study confirmed that comorbidities, particularly dementia and diabetes mellitus, significantly influence functional outcomes 6 months after fragility hip fracture surgeries.
Physiological uterine uptake of 68Ga-FAPI-04 may limit its application in uterine pathology
Authors: Zhang Xiao , Song Wenyu , Qin Chunxia , Song Yangmeihui , Liu Fang , Hu Fan , Lan Xiaoli 
Published: February 2021

Abstract
Purpose68Ga-labeled fibroblast activation protein inhibitor (68Ga-FAPI) has been useful in the imaging of desmoplastic reaction in different tumors, as well as fibrosis in inflammatory diseases. As we have found that most female patients showed avid uterine uptake of 68Ga-FAPI, we sought to further investigate the physiological uptake of 68Ga-FAPI by the uterus and analyze its characteristics in women of childbearing age, in perimenopause, and postmenopause.MethodsWe retrospectively reviewed the image data of female patients who had undergone whole-body 68Ga-FAPI-04 PET/MRI at our institute between May 22 and December 16, 2020. The clinical information of the patients, including age, stage of menstrual cycle if present, and gynecologic history, was collected. Uterine volume was calculated from MR images. The characteristics of 68Ga-FAPI-04 uterine uptake were recorded. If the patients also had undergone 18F-FDG PET/CT imaging, those image features were also recorded. The relationship of age, uterine size, surgical history, presence of leiomyomas, and 18F-FDG uptake to 68Ga-FAPI-04 uptake were further analyzed.ResultsForty-three female patients were included in this study. The number of cases with uterine malignancy and non-malignancy were two and 41, respectively. Twenty-seven patients in the benign disease group underwent simultaneous 18F-FDG PET/CT scans. Of 41 patients with benign uterine pathology, 13 patients were of reproductive age, six were perimenopausal, and 22 were postmenopausal. A total of 25 patients had undergone invasive operations, and six patients had uterine fibroids under examination. Lower 68Ga-FAPI-04 uptake (6.58 ± 3.39, n = 22) was noted in postmenopausal women than in reproductive and perimenopausal females (13.32 ± 3.2 and 12.98 ± 3.01, respectively, P < 0.05). The invasive operation or accompany with uterine fibroids may increase 68Ga-FAPI-04 uptake. 68Ga-FAPI-04 uptake was also associated with uterine volume (P < 0.01). There was no correlation between 18F-FDG and 68Ga-FAPI-04 accumulation in the uterus. Two patients with malignant lesions involving the uterus showed completely different imaging features on 68Ga-FAPI-04.ConclusionPhysiological uterine uptake of 68Ga-FAPI-04 limits its diagnostic value in gynecologic diseases. Age, uterine fibroids and uterine volume may influence the uptake of 68Ga-FAPI-04 in uterus. More patients with various uterine diseases could be involved to provide more differential diagnostic information.Clinical Trial RegistrationNCT04605939 and NCT04554719.
Antiparasitic potential of alternative treatments against larval stages of Lernaea cyrpiancea
Authors: Furtado William Eduardo , Cardoso Lucas , Medeiros Paula Brando de , Lehmann Nicollas Breda , Bertaglia Elisabeth de Aguiar , Marchiori Natalia da Costa , Bertoldi Fabiano Cleber , Martins Maurício Laterça 
Published: February 2021

Abstract
This study evaluated the potential of alternative treatments against larval stages of Lernaea cypriancea. For in vitro test, the nanoemulsified oils of Pinus sp. acicule and resin were evaluated, along with Biogermex® (commercial product based on citrus biomass). For this, the motility of five larvae of the same stage (nauplii or copepodite) were evaluated in a 96-well microplate. Using the best results, on the in vivo test, fries of Rhamdia quelen were submitted to a long-term immersion bath (96 h) containing different concentrations of the product diluted directly in the water. It was possible to notice the antiparasitic potential of the resin and the acicule of Pinus sp., as well as the citrus biomass extract against the parasites. The nanoemulsified oils successfully inhibited the development of nauplii (10 mg L− 1 in 24 h) and the fries showed to be tolerant to the presence of the compound (LC50 96h − 16.74 mg L− 1). The concentration of 30.5 mg L− 1 of Biogermex® eliminated the copepodites within 24 h, being more efficient than Pinus sp. when tested at the same stage, at the times analyzed. The results obtained indicate a potential use of these compounds as prophylactic agents against L. cyprinacea.