bluuebunny/crossref_metadata_2025_split huggingface dataset:
Usefulness of endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in elderly patients: a single-center retrospective cohort study
Authors: Ishido Kenji , Tanabe Satoshi , Katada Chikatoshi , Kubota Yo , Furue Yasuaki , Wada Takuya , Watanabe Akinori , Koizumi Wasaburo 
Published: February 2021

Abstract

Background
The effectiveness of endoscopic treatment for superficial esophageal squamous cell carcinoma in the elderly is unclear.

Methods
We retrospectively studied efficacy and safety of endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in 358 patients at our hospital from July 2005 to December 2018. Patients were divided into elderly (≥75 years) and young (≤74 years) groups. Efficacy was evaluated based on overall survival and disease-specific survival, whereas safety was investigated based on the frequency of endoscopic submucosal dissection-related adverse events.

Results
The median observation period was 50 months. The elderly group comprised 111 patients, and young group comprised 247 patients. In the elderly and young groups, 76 (68.5%) and 159 (64.4%) underwent curative resection (P = 0.450), 8 (7.2%) and 34 (13.8%) underwent non-curative resection plus additional treatment and 12 (10.8%) and 15 (6.0%) underwent follow-up, respectively. The frequency of additional treatment for non-curative resection was significantly lower in the elderly group (P = 0.023). The 3-year overall survival of the elderly and young groups was 85.6 and 94.1%, respectively (P = 0.003). The 3-year disease-specific survival of the elderly and young groups was 98.4 and 98.5% (P = 0.682), respectively. The frequency of endoscopic submucosal dissection-related adverse events did not differ significantly between the groups (P = 0.581). The Charlson Comorbidity Index ≥2 was an independent prognostic factor for survival in the elderly group (P = 0.010; hazard ratio, 5.570; 95% confidence interval, 1.519–20.421).

Conclusions
Endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in elderly patients is as safe as that for young patients. The evaluation of Charlson Comorbidity Index was considered to help estimate the prognosis of elderly patients.
Mitotic rate in node-positive stage III melanoma: it might be as important a prognostic factor as node number
Authors: Tas Faruk , Erturk Kayhan 
Published: February 2021

Abstract

Background
Stage III melanoma is a heterogenous disease, and the number of tumor-involved lymph nodes is the most significantly unfavorable prognostic indicator for relapse and outcome. The aim of this study is to investigate the possible effects of the various clinicopathological factors on the course of node-positive stage III disease.

Methods
A total of 389 node-positive stage III cutaneous melanomas were included in the study and analyzed retrospectively. All underwent pathological nodal staging by sentinel lymph node biopsy or elective lymph node dissection.

Results
The group was male-dominant (59%) and the median age was 50 years. The largest group of patients was N1 (n = 221, 56.8%) followed by N2 (n = 105, 27.0%) and N3 (n = 63, 16.2%). N1 melanomas were less frequently associated with relapses than melanomas with multiple lymph node metastases (P = 0.05). The 5-year relapse-free survival rate was 37.9%. The melanomas with multiple lymph nodes metastases (P = 0.01), higher mitotic rate (P = 0.005) and ulceration (P = 0.02) had worse RFS. In the multivariate analysis only the significances of the N2–N3 stage (P = 0.016) and higher mitosis (P = 0.012) persisted. The severe lymph node metastasis (N2–N3) was associated with a higher mortality rate in comparison with the single nodal involvement (P = 0.05). The 5-year overall survival rate was 52.1%. Presence of relapse (P = 0.0001), higher mitotic rate (P = 0.03) and N2–N3 stage (P = 0.04) were inversely correlated with the overall survival. When relapse was included in the multivariate analysis, it was the only significant prognostic factor on survival (P = 0.0001), whereas mitosis became the only significant factor on survival with the exclusion of relapse from the multivariate analysis (P = 0.031).

Conclusion
In node-positive stage III melanoma, tumor mitotic rate might be just as significant a prognostic indicator as the metastatic lymph node number.
Perianal abscess due to a long fish bone: a case report
Authors: Ikeda Taro , Konaka Ryunosuke , Adachi Yukari , Matsumoto Akiko , Harada Naoki , Wada Takahiro , Mitsutsuji Masaaki , Samizo Masahiro 
Published: February 2021

Abstract
Few articles have reported cases of perianal abscess due to ingested foreign bodies. Herein, we report a case of perianal abscess due to a long fish bone.
A 72-year-old man who was toothless and wore a denture had a chief complaint of anal pain. His left-side buttock had swelling and redness. Computed tomography revealed a perianal abscess on his left-side buttock and high-intensity linear structure in the abscess cavity. We made a diagnosis of perianal abscess due to a fish bone and performed an emergency operation. We opened the abscess cavity and removed the 5 cm fish bone from the cavity. After drainage of the abscess cavity and antibiotic administration, he was discharged from our hospital on day 8.
A long fish bone could cause perianal abscesses. Rapid diagnosis and ensuring fish bone removal are important to prevent sepsis.
The Key Elements of Medical Negligence—Duty
Authors: Luther Gregg W 
Published: February 2021

Abstract
American tort law (a.k.a. negligence) is designed to be flexible and elastic to adapt to changes in time and public policy. It provides a structure of elements and factors to be applied to each case's specific facts on a case-by-case basis. The purpose of this structure is to achieve as much uniformity as possible in the application of tort law. One side effect is that this structure makes predicting the outcome difficult because of so many variables. In addition, there is no national tort law. Instead, each state has developed its own law in the area of torts, which has resulted in differing exceptions and requirements based on where the medical care was given. The purpose of this article is to explain the first element of a negligence case—“duty to use care”—and its accompanying factors/variables.
The first element that must be established in a medical negligence case is a duty to use care. In short, this means the physician must use a certain level of care in providing medical services. The physician typically owes her or his patient the duty to exercise care. However, there are special situations in which a physician may not owe a duty of care and thus cannot be held liable for medical negligence. This article is designed to provide an overview of the most common situations and summarizes the processes to determine whether a duty is owed.
A three-dimensional velocity of an erupting prominence prior to a coronal mass ejection
Authors: Gutierrez Maria V , Otsuji Kenichi , Asai Ayumi , Terrazas Raul , Ishitsuka Mutsumi , Ishitsuka Jose , Nakamura Naoki , Yoshinaga Yusuke , Morita Satoshi , Ishii Takako T , Ueno Satoru , Kitai Reizaburo , Shibata Kazunari 
Published: February 2021

Abstract
We present a detailed three-dimensional (3D) view of a prominence eruption, coronal loop expansion, and coronal mass ejections (CMEs) associated with an M4.4 flare that occurred on 2011 March 8 in the active region NOAA 11165. Full-disk Hα images of the flare and filament ejection were successfully obtained by the Flare Monitoring Telescope (FMT) following its relocation to Ica University, Peru. Multiwavelength observation around the Hα line enabled us to derive the 3D velocity field of the Hα prominence eruption. Features in extreme ultraviolet were also obtained by the Atmospheric Imager Assembly onboard the Solar Dynamic Observatory and the Extreme Ultraviolet Imager on board the Solar Terrestrial Relations Observatory - Ahead satellite. We found that, following collision of the erupted filament with the coronal magnetic field, some coronal loops began to expand, leading to the growth of a clear CME. We also discuss the succeeding activities of CME driven by multiple interactions between the expanding loops and the surrounding coronal magnetic field.
Christian Hospitality and the Case for Religious Refuge in Interregnum England
Authors: Fradkin Jeremy 
Published: February 2021

Abstract
This article shows how English supporters of Jewish immigration in the 1650s articulated a universal model of Christian hospitality for all foreigners fleeing religious persecution, regardless of whether they adhered to the Protestant faith of their English hosts. It thus urges a reconsideration of the widespread assumption that European Christians in this era were willing only to admit their own co-religionists as refugees. At the same time, however, the article points to the 1650s as a pivotal turning point in the relationship between the resettlement of refugees and the development of the British Empire. Severe crises of depopulation in newly conquered Ireland and Jamaica prompted a proliferation of schemes to resettle refugees in those two spaces. Empire, it was argued, would allow the English to be benevolent and charitable without having to share their own country with people different from themselves.
Constraints on the Formation of the Giant Daheishan Porphyry Mo Deposit (NE China) from Whole-Rock and Accessory Mineral Geochemistry
Authors: Xing Kai , Shu Qihai , Lentz David R 
Published: February 2021

AbstractThere are more than 80 porphyry (or skarn) Mo deposits in northeastern China with Jurassic or Cretaceous ages. These are thought to have formed mainly in a continental arc setting related to the subduction of the Paleo-Pacific oceanic plate in the Jurassic and subsequent slab rollback in the early Cretaceous. The Jurassic Daheishan porphyry Mo deposit is one of the largest Mo deposits in NE China, which contains 1·09 Mt Mo with an average Mo grade of 0·07 %. To better understand the factors that could have controlled Mo mineralization at Daheishan, and potentially in other similar porphyry Mo deposits in NE China, the geochemical and isotopic compositions of the ore-related granite porphyry and biotite granodiorite, and the magmatic accessory minerals apatite, titanite and zircon from the Daheishan intrusions, were investigated so as to evaluate the potential roles that magma oxidation states, water contents, sulfur and metal concentrations could have played in the formation of the deposit. Magmatic apatite and titanite from the causative intrusions show similar εNd(t) values from –1·1 to 1·4, corresponding to TDM2 ages ranging from 1040 to 840 Ma, which could be accounted for by a mixing model through the interaction of mantle-derived basaltic melts with the Precambrian lower crust. The Ce and Eu anomalies of the magmatic accessory minerals have been used as proxies for magma redox state, and the results suggest that the ore-forming magmas are highly oxidized, with an estimated ΔFMQ range of +1·8 to +4·1 (+2·7 on average). This is also consistent with the high whole-rock Fe2O3/FeO ratios (1·3–26·4). The Daheishan intrusions display negligible Eu anomalies (Eu/Eu* = 0·7–1·1) and have relatively high Sr/Y ratios (40–94) with adakitic signatures; they also have relatively high Sr/Y ratios in apatite and titanite. These suggest that the fractionation of amphibole rather than plagioclase is dominant during the crystallization of the ore-related magmas, which further indicates a high magmatic water content (e.g. &gt;5 wt%). The magmatic sulfur concentrations were calculated using available partitioning models for apatite from granitoids, and the results (9–125 ppm) are indistinguishable from those for other mineralized, subeconomic and barren intrusions. Furthermore, Monte Carlo modelling has been conducted to simulate the magmatic processes associated with the formation of the Daheishan Mo deposit, and the result reveals that a magma volume of ∼280 km3 with ∼10 ppm Mo was required to form the Mo ores containing 1·09 Mt Mo in Daheishan. The present study suggests that a relatively large volume of parental magmas with high oxygen fugacities and high water contents is essential for the generation of a giant porphyry Mo deposit such as Daheishan, whereas a specific magma composition (e.g. with unusually high Mo and/or S concentrations) might be less critical.
Prediabetes, undiagnosed diabetes and diabetes risk in Italy in 2017–2018: results from the first National screening campaign in community pharmacies
Authors: Brunetti P , Baldessin L , Pagliacci S 
Published: February 2021

Abstract

Background
Effective policies for diabetes prevention remain urgent. We conducted a mass screening campaign in Italy to identify subjects potentially having undiagnosed diabetes, prediabetes or at diabetes risk.

Methods
This cohort study was conducted in community pharmacies joining the unitary National federation of pharmacy holders (Federfarma) and participating in the 7-day screening campaign ‘DiaDay’ in 2017–2018. Capillary blood glucose levels and the risk of developing diabetes in 10 years (through the Finnish Diabetes Risk Score) were assessed.

Results
145 651 volunteers aged ≥20 years without known diabetes were screened at 5671 community pharmacies in 2017 and 116 097 at 5112 in 2018. Overall, 3.6% had glucose values suggestive of undiagnosed diabetes; under fasting conditions (N = 94 076), 39.9% and 16.4% had values suggestive of prediabetes by the American Diabetes Association and the World Health Organization criteria, respectively. Of those without diabetes (N = 252 440), 19.2% had scores compatible with a high risk (1:3) and 2.7% with a very high risk (1:2) of developing the disease; in the prediabetes group, the risk rose with higher impaired fasting glucose values.

Conclusions
DiaDay, the first National screening campaign, highlights the need to screen the population and the key role of the pharmacist both in screening activities and education promotion.
Effects of high-protein distillers dried grains on growth performance of nursery pigs
Authors: Cemin Henrique S , Tokach Mike D , Dritz Steve S , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Wilken Mallorie F 
Published: February 2021

AbstractA total of 300 pigs (DNA 400 × 200, Columbus, NE), initially 11.1 kg, were used in a study to evaluate the effects of increasing amounts of high-protein distillers dried grains (HP DDG) on growth performance and to estimate its energy value relative to corn. Pigs were weaned, placed in pens with five pigs each, and fed a common diet for 21 d after weaning. Then, pens were assigned to treatments in a randomized complete block design. There were 5 treatments with 12 replicates per treatment. Treatments consisted of 0, 10, 20, 30, or 40% HP DDG, formulated by changing only the amounts of corn and feed-grade amino acids. Pigs were weighed weekly for 21 d to evaluate average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F). Caloric efficiency was obtained by multiplying ADFI by kcal of net energy (NE) per kg of diet and dividing by ADG. The NE values for corn and soybean meal were obtained from NRC (2012), and initial estimates for HP DDG NE were derived from the Noblet et al. (1994) equation. The energy of HP DDG was estimated based on caloric efficiency relative to the diet without HP DDG. Pigs fed diets with increasing HP DDG had a linear decrease (P &lt; 0.01) in ADG, ADFI, and final body weight. There was a tendency for a quadratic response (P = 0.051) in G:F, with the greatest G:F observed for pigs fed diets with 40% HP DDG. There was a linear reduction (P &lt; 0.05) in caloric efficiency with increasing amounts of HP DDG, indicating the initial NE estimate of HP DDG was underestimated. The use of caloric efficiency to estimate the energy value of HP DDG presents several limitations. This approach assumes that the NE values of corn and soybean meal are accurate and does not take into account possible changes in body composition, which can influence the G:F response as leaner pigs are more efficient. In conclusion, increasing HP DDG in the diet linearly decreased ADG and ADFI. Using caloric efficiency to estimate energy content relative to corn, the HP DDG used in this study was estimated to be 97.3% of the energy value of corn. Direct or indirect calorimetry is needed to confirm this value.
Evaluation of the effect of border closure on COVID-19 incidence rates across nine African countries: an interrupted time series study
Authors: Emeto Theophilus I , Alele Faith O , Ilesanmi Olayinka S 
Published: February 2021

Abstract

Background
Border closure is one of the policy changes implemented to mitigate against coronavirus disease 2019 (COVID-19). We evaluated the effect of border closure on the incidence rate of COVID-19 across nine African countries.

Methods
An interrupted time series analysis was used to assess COVID-19 incidence rates in Egypt, Tunisia, Democratic Republic of the Congo (DRC), Ethiopia, Kenya, Ghana, Nigeria, Senegal and South Africa (SA). Data were collected between 14 February and 19 July 2020 from online data repositories. The linear trend and magnitude of change were evaluated using the itsa function with ordinary least-squares regression in Stata with a 7-d deferred interruption point, which allows a period of diffusion post-border closure.

Results
Overall, the countries recorded an increase in the incidence rate of COVID-19 after border closure. However, when compared with matched control groups, SA, Nigeria, Ghana, Egypt and Kenya showed a higher incidence rate trend. In contrast, Ethiopia, DRC and Tunisia showed a lower trend compared with their controls.

Conclusions
The implementation of border closures within African countries had minimal effect on the incidence of COVID-19. The inclusion of other control measures such as enhanced testing capacity and improved surveillance activities will reveal the effectiveness of border closure measures.
SARS-CoV-2 genomic analyses in cancer patients reveal elevated intrahost genetic diversity
Authors: Siqueira Juliana D , Goes Livia R , Alves Brunna M , Carvalho Pedro S de , Cicala Claudia , Arthos James , Viola João P B , de Melo Andréia C , Soares Marcelo A 
Published: February 2021

Abstract
Numerous factors have been identified to influence susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and disease severity. Cancer patients are more prone to clinically evolve to more severe COVID-19 conditions, but the determinants of such a more severe outcome remain largely unknown. We have determined the full-length SARS-CoV-2 genomic sequences of cancer patients and healthcare workers (non-cancer controls) by deep sequencing and investigated the within-host viral population of each infection, quantifying intrahost genetic diversity. Naso- and oropharyngeal SARS-CoV-2+ swabs from 57 cancer patients and 14 healthcare workers from the Brazilian National Cancer Institute were collected in April to May 2020. Complete genome amplification using ARTIC network V3 multiplex primers was performed followed by next-generation sequencing. Assemblies were conducted in Geneious R11, where consensus sequences were extracted and intrahost single nucleotide variants were identified. Maximum likelihood phylogenetic analysis was performed using PhyMLv.3.0 and lineages were classified using Pangolin and CoV-GLUE. Phylogenetic analysis showed that all but one strain belonged to clade B1.1. Four genetically linked mutations known as the globally dominant SARS-CoV-2 haplotype (C241T, C3037T, C14408T and A23403G) were found in the majority of consensus sequences. SNV signatures of previously characterized Brazilian genomes were also observed in most samples. Another 85 SNVs were found at a lower frequency (1.4%–19.7%) among the consensus sequences. Cancer patients displayed a significantly higher intrahost viral genetic diversity compared to healthcare workers. This difference was independent of SARS-CoV-2 Ct values obtained at the diagnostic tests, which did not differ between the two groups. The most common nucleotide changes of intrahost SNVs in both groups were consistent with APOBEC and ADAR activities. Intrahost genetic diversity in cancer patients was not associated with disease severity, use of corticosteroids, or use of antivirals, characteristics that could influence viral diversity. Moreover, the presence of metastasis, either in general or specifically in the lung, was not associated with intrahost diversity among cancer patients. Cancer patients carried significantly higher numbers of minor variants compared to non-cancer counterparts. Further studies on SARS-CoV-2 diversity in especially vulnerable patients will shed light onto the understanding of the basis of COVID-19 different outcomes in humans.
Coding of chromatic spatial contrast by macaque V1 neurons
Authors: De Abhishek , Horwitz Gregory D. 
Published: February 2021

ABSTRACTColor perception relies on spatial comparisons of chromatic signals, but how the brain performs these comparisons is poorly understood. Here, we show that many V1 neurons compare signals across their receptive fields (RF) using a common principle. We estimated the spatial-chromatic RFs of each neuron, and then measured neural selectivity to customized colored edges that were sampled using a novel closed-loop technique. We found that many double-opponent (DO) cells, which have spatially and chromatically opponent RFs, responded to chromatic contrast as a weighted sum, akin to how simple cells respond to luminance contrast. Other neurons integrated chromatic signals non-linearly, confirming that linear signal integration is not an obligate property of V1 neurons. The functional similarity of DO and simple cells suggests a common underlying neural circuitry, promotes the construction of image-computable models for full-color image representation, and sheds new light on V1 complex cells.
Sparcle: assigning transcripts to cells in multiplexed images
Authors: Prabhakaran Sandhya , Nawy Tal , Pe’er’ Dana 
Published: February 2021

AbstractBackgroundImaging-based spatial transcriptomics has the power to reveal patterns of single-cell gene expression by detecting mRNA transcripts as individually resolved spots in multiplexed images. However, molecular quantification has been severely limited by the computational challenges of segmenting poorly outlined, overlapping cells, and of overcoming technical noise; the majority of transcripts are routinely discarded because they fall outside the segmentation boundaries. This lost information leads to less accurate gene count matrices and weakens downstream analyses, such as cell type or gene program identification.ResultsHere, we present Sparcle, a probabilistic model that reassigns transcripts to cells based on gene covariation patterns and incorporates spatial features such as distance to nucleus. We demonstrate its utility on both multiplexed error-robust fluorescence in situ hybridization (MERFISH) and single-molecule FISH (smFISH) data.ConclusionsSparcle improves transcript assignment, providing more realistic per-cell quantification of each gene, better delineation of cell boundaries, and improved cluster assignments. Critically, our approach does not require an accurate segmentation and is agnostic to technological platform.