bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chiropractic website claims related to non-musculoskeletal conditions: a cross-sectional study
Authors: Goncalves Guillaume , Fleuriau Philippe , Cheron Charlène , Picchiottino Mathieu , Pigeon Sylvain , Jensen Rikke Krüger 
Published: June 2021

Abstract
Introduction: Chiropractors frequently use spinal manipulation therapy as a treatment modality in the management of musculoskeletal (MSK) conditions such as spinal pain and in some cases in the treatment of non-MSK disorders. The latter is not supported by evidence. This study aimed to investigate the extent of non-MSK website claims from French chiropractors to assess if websites were aligned with current recommendations on evidence-based practice.Method: This was a cross-sectional study of a representative sample of French chiropractors. Information on non-MSK conditions were collected from chiropractic professional websites by two independent observers. For each non-MSK condition, it was noted whether a clarifying explanation justifying the mentioning of the condition was available. In addition, data on professional association affiliation status, country of education, years since graduation, and special clinical focus (e.g., seniors, children) were collected. Results: A total of 287 randomly selected websites were included in the study corresponding to 22% of all chiropractors registered in France. One or more of 42 different non-MSK conditions were identified on 231 websites, of which 5 (2.2%) provided a clarifying explanation. 226 (79%) websites mentioned a non-MSK condition without a clarifying explanation. The non-MSK conditions most often mentioned were sleep problems, constipation/digestive problems, unease/discontent in children and menstrual cramps/pains. A larger proportion of the websites advertising treatment for non-MSK disorders were found among those claiming a special clinical focus on infants/children, seniors, pregnant women or athletes compared to those that did not. Also, a larger proportion of chiropractors who were affiliated with a professional association were advertising treatment for non-MSK disorders compared to those who were not affiliated.Conclusion: Eight out of ten chiropractic websites mentioned one or more non-MSK diagnoses or symptoms. It is unclear if this reflects inaccurate communication or if treatments for non-MSK conditions are provided by the chiropractors.
CDK10 Regulates Gastric Cancer Metastasis By Inhibiting lncRNA-C5ORF42-5 Via Phosphorylation Level of AKT/ERK
Authors: Deng Zi-Jian , Chen Dong-Wen , Chen Xi-Jie , Fang Jia-Ming , Xv Liang , Peng Jun-Sheng , Chen Shi 
Published: June 2021

Abstract
Background: Gastric cancer is the fourth most common malignant disease. Both CDK10 and long noncoding RNAs (lncRNAs) have been found to exert biological functions in multiple cancers. However, it is still unclear whether CDK10 represses tumor progression in gastric cancer by reducing potential targeting lncRNAs.Methods: The functions of CDK10 and lncRNA-C5ORF42-5 in proliferation, invasion and migration were assessed by MTS assays, colony formation assays, cell cycle and apoptosis assays, Transwell assays, wound healing assays and animal experiments. We used high-throughput sequencing to confirm the existence of lncRNA-C5ORF42-5 and quantitative real-time PCR was used to evaluate lncRNA expression. Then, with RNA-seq sequencing as well as GO function and KEGG enrichment analysis, we identified the signaling pathways in which lncRNA-C5ORF42-5 was involved in gastric cancer. Finally, western blotting was used to identify the genes regulated by lncRNA-C5ORF42-5.Results: Our results showed that CDK10 is expressed at relatively low levels in gastric cancer cell lines and inhibits the progression of gastric cancer cells both in vitro and in vivo. Next, based on high-throughput sequencing, we identified a novel lncRNA, lncRNA-C5ORF42-5, in the stable CDK10-overexpressing cell line compared with the CDK-knockdown cell line and their controls. Additionally, we confirmed that lncRNA-C5ORF42-5 acts as an oncogene to promote metastasis in gastric cancer in vitro and in vivo. We then ascertained that lncRNA-C5ORF42-5 is a major contributor to the function of CDK10 in gastric cancer metastasis by upregulating lncRNA-C5ORF42-5 to reverse the effects of CDK10 overexpression. Finally, we explored the mechanism by which lncRNA-C5ORF42-5 overexpression affects gastric cancer cells to elucidate whether lncRNA-C5ORF42-5 may increase the activity of the SMAD pathway of BMP signaling and promote the expression of EMT-related proteins, such as E-cadherin. Additionally, overexpression of lncRNA-C5ORF42-5 affected the phosphorylation levels of AKT and ERK.Conclusion: Our findings suggest that CDK10 overexpression represses gastric cancer tumor progression by reducing lncRNA-C5ORF42-5 and hindering activation of the related proteins in metastatic signaling pathways, which provides new insight into developing effective therapeutic strategies in the treatment of metastatic gastric cancer.
Ascogregarina Taiwanensis Interfere in the Performance of Aedes Albopictus and in the Susceptibility of Aedes Aegypti to Temephos and Azadirachta Indica Oil
Authors: Prophiro Josiane Somariva , Pereira Thiago Nunes , de Oliveira Joice Guilherme , Costa Felipe Allan Silva da , Júnior Harry Luiz Pilz , de Lemos Alessandra Bittencourt , Silva Onilda Santos da , Silva Mario Antônio Navarro da 
Published: June 2021

Abstract
Background: Aedes albopictus and Aedes aegypti are mosquitoes commonly adapted to tropical and subtropical regions. These vectors can transmit different types of arboviruses causing a serious concern to public health. New alternatives for the vector/arboviruses control are emerging, and in this sense the protozoan Ascogregarina taiwanensis may present potential as a biological control agent against these mosquitoes. Methods: To evaluate the effects of protozoan A. taiwanensis, mosquitoes were parasitized with a solution containing oocysts and evaluated to lifetime, fertility, fecundity for Ae. albopictus and for Ae. aegypti interaction with Azadirachta indica and Temephos. Results: In this work it was possible to observe the protozoan morphology in mosquitoes Ae. albopictus, as well its negative influence on mortality, 73% and non-parasitized was 44%. The number of eggs oviposited by parasitized females of Ae. albopictus was lower (3,490) than for the non-parasitized females (5,586). In addition, the hatchability and/or viability of these eggs were also lower for the parasitized females (63%) than the non-parasitized ones (74%). For Ae. aegypti mosquitoes, a synergism between the use of A. taiwanensis associated with a chemical insecticide and a botanical insecticide was observed. The results demonstrate that when Ae. aegypti larvae was parasitized by A. taiwanensis and exposed to the oil of Az. indica or to the organophosphate Temephos present a greater mortality. Conclusion: It was notable that A. taiwanensis can be a potential for biological control and adjuvant of insecticides. We also provide important information about the maintenance of A. taiwanensis in laboratory.
Tocilizumab is Associated with Increased Risk of Fungal Infections Among Critically Ill Patients with COVID-19 and Acute Renal Failure: An Observational Cohort Study.
Authors: Burger Barrett J , Epps Sarenthia M. , Cardenas Victor M. , Jagana Rajani , Meena Nikhil K. , Atchley William T. 
Published: June 2021

Abstract
Research QuestionDoes treatment with tocilizumab increase the risk of a fungal infection in critically ill patients with coronavirus-19?BackgroundNumerous therapies have been re-evaluated as possible treatment options for coronavirus-2019 caused by severe acute respiratory syndrome coronavirus-2. Tocilizumab is a humanized monoclonal antibody directed against the interleukin-6 receptor that has been proposed as a therapy for patients with severe coronavirus-19 pneumonia. The immunomodulatory effects of tocilizumab may have the unintended consequence of predisposing recipients to secondary infections. We sought to assess the risk of invasive fungal disease and the therapeutic impact of tocilizumab on hospital length of stay, duration of mechanical ventilation, and intensive care unit length of stay in critically ill patients with severe coronavirus-19.MethodsRecords of critically ill patients with coronavirus-2019 admitted from March to September 2020 at our institution were reviewed. The risk for fungal infections, intensive care unit length of stay, hospital length of stay, and duration of mechanical ventilation in those that received tocilizumab in addition to standard coronavirus-2019 treatments was assessed. ResultsFifty-six critically ill patients treated with dexamethasone and remdesivir for coronavirus-2019 were included, of which 16 patients also received tocilizumab. The majority of the cohort was African American, Asian, or other ethnic minorities (53.6%). Invasive fungal infections occurred in 10.7% of all patients and infection rates were significantly higher in the tocilizumab group than in the control group (31.2% vs 2.5%, Risk Difference [RD]= 28.8%, p<0.01). The increased risk in the tocilizumab group was strongly associated with renal replacement therapy. There was a dose-response relation between the risk of fungal infection and doses of tocilizumab, with 2.5% of infections occurring with zero doses, 20% with a single dose (RD=17.5%), and 50% with two doses (RD=47.5%) (trend test p<0.001). In addition, ICU LOS (23.4 days v 9.0 days, p <0.01), duration of mechanical ventilation (18.9 v. 3.5 days, p=0.01), and hospital LOS (29.1 v. 15.5, p <0.01) were increased in patients that received tocilizumab. ConclusionsRepurposed therapies, such as tocilizumab, may have a role in the treatment of severe coronavirus-2019 pneumonia but safety concerns remain. In this cohort, tocilizumab treatment was associated with an increased risk of fungal infection in those that were critically ill and received renal replacement therapy. Tocilizumab was also associated with increased ICU and hospital LOS and duration of mechanical ventilation.
Child Injury Burden and Risk Profile in a Bangladeshi Rural Community: an Age-Group Related Overview
Authors: Afreen Nawroz , Billah Mallick Masum , Flora Meerjady Sabrina 
Published: June 2021

Abstract
Background:Child injury was a growing public health problem in Bangladesh. Recent information on variation in patterns of non-fatal injury, severity, and risk-profile in rural Bangladeshi children was unavailable. This study aimed to determine current burden of non-fatal injury and risks considering severity in disability-days in child age-groups in a rural community. Methods: This cross-sectional study was conducted in randomly chosen children (<18-year) from selected households of village-clusters with pretested semi-structured questionnaires and checklist for household-environment between May-June 2018 in selected sub-district. Besides descriptive analysis, Chi-square test and Odds Ratio with 95% Confidence Interval (CI) were calculated for identifying risk profiles. An injury was considered minor when regular activities were hampered <30 days and major when ≥30 days. A minor injury must have occurred in the previous three months and a major injury in previous year.Results:For 918 children, prevalence of major and minor injury were 2.4 (95% CI 1.5-3.6) and 7.4 (95% CI 5.8-9.3) with highest in 5-9 and 1-4-year respectively. Commonest pattern was fall injury, followed by cut injury, transport injury and burn for both major and minor type, with variations in age-groups. For major-injury, risks included male gender (OR 4.6, 95% CI 1.5-18.9), dumped garbage (OR 5.0, 95% CI 1.5-26.7), and exclusive/complementary use of non-electric power-source (OR 5, 95% CI 1.2-16.1). For minor-injury, risks for all age groups were ≥5 working hours/day of a mother (OR 2.8, 95% CI 1.2-7.2), supervision of <6 children during her works (OR 3.2, 95% CI 1.05-13) and open fireplaces (OR 3.2, 95% CI 1.3-7.2). When analysis of minor injury considering specific age-groups was done, mothers’ ≥5 working hours and child-supervision were particularly found risky for 1-4 year; open fireplaces for 5-9 year; along with male gender, uneducated mothers for 10-14 year. Conclusions: Prevalence of major and minor injuries were though lower, still required intervention for further reduction and the variabilities in burden, patterns, severity and risks among age-groups could be considered for prioritizing interventions. As risk characteristics were not well established in a cross-sectional study, further case-control study with a qualitative part was recommended for assimilation of risk-profile to guide policy formulation.
Dark matter density extraction using Convolutional Neural Networks
Authors: Dongre Pratik 
Published: June 2021

Abstract
Ever since its discovery back in 1964 Cosmic Microwave Background (CMB) has been of great interest to cosmologists and played a crucial role in understanding and studying the early universe .One of the most interesting topic of current interest is dark matter and its existence is by now well established. By analyzing the CMB data we can estimate the dark matter density of the universe.With vast amount of astronomical data already present and a more vast amount which is to come in future, Machine Learning techniques can provide a variety of benefits in astrophysical and cosomological research. Here I explore the use of deep learning to estimate dark matter density. I have used convolutional neural networks in this paper. I have used simulated CMB temprature maps as a dataset to train the neural networks and correlate the dark matter density from the power spectrum of the corrseponding simlutaed CMB temprature map.
Serum and Gene Expression Profile of Cytokines Following Combination of Yoga Training and Vitamin D Supplementation in Breast Cancer Survivors
Authors: Naderi Mehdi , Kordestani Hajar , Sahebi Zahra , Khedmati-zareh Vahid , Amani-Shalamzari Sadegh , Kaviani Mojtaba , Wiskemann Joachim , Shamsi Mahdieh Molanouri 
Published: June 2021

Abstract
Background

This study aimed to examine the effect of a combination of yoga training with high vitamin D dose supplementation on expression and systemic levels of inflammatory cytokines and psychophysical status of breast cancer survivors.
Methods

Thirty volunteered breast cancer survivors (48 ± 8 yrs.) were randomly allocated to a high dose (4000 IU) of vitamin D supplementation (HD) group (n = 10), yoga with a high dose of vitamin D (YHD) group (n = 10), and yoga with a low dose (2000 IU) of vitamin D (YLD) group (n = 10). Participants performed the Hatha yoga style for 12 weeks, twice a week. Blood samples, quality of life (Qol) questionnaire, and physical performance tests were taken before and after the intervention
Results

Body fat percentage (ηp2 = 0.36), handgrip strength (ηp2 = 0.41), and Qol indicators include global health (ηp2 = 0.54), functional scales (ηp2 = 0.49), and symptoms scales (ηp2 = 0.50) were significantly improved in the both YHD and YLD groups compared to the HD group (p < 0.05). Also, interleukin-10 (IL-10) levels were markedly increased in the Y-HVD group compared to the Y-LVD and HVD groups. Moreover, there were significant decreases in tumor necrosis factor-α (TNF-α) and interleukin-6 levels in the Y-HVD group after the intervention. The anti-inflammatory index (IL-10/TNF-α) was significantly increased in both the yoga groups (P < 0.05).
Conclusion

Yoga promotes physical and psychological fitness and, in combination with a high dose of vitamin D, improves the cytokine profile, which can effectively manage the side effects associated with cancer.
The Nucleolar DExD/H Protein Hel66 is Involved in Ribosome Biogenesis in Trypanosoma Brucei
Authors: Bakari-Soale Majeed , Ikenga Nonso Josephat , Scheibe Marion , Butter Falk , Jones Nicola Gail , Kramer Susanne , Engstler Markus 
Published: June 2021

Abstract
The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.
Predictive Factors for Sperm Retrieval in Males With Non-obstructive Azoospermia
Authors: Hsu Wei-Hsun , Chen Marcelo , Chiang Pai-Kai , Tsai Wei-Kung , Chiu Allen W , Chen Yi-Zhong 
Published: June 2021

Abstract
The aim of the current study was to investigate the predictive markers for males with non-obstructive azoospermia (NOA) before they received conventional testicular sperm extraction (cTESE) or microdissection testicular sperm extraction (microTESE). Between January 2010 and December 2020, a total of 56 patients who received cTESE or microTESE surgery at the Urology department of the MacKay Memorial Hospital were included. Our univariate analysis revealed that the following parameters was associated with sperm retrieval: Follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, testicular volume, histopathology of maturation arrest and Sertoli cell-only. The multivariate analysis showed that Sertoli cell-only was significantly less likely to harvest spermatozoa than normal spermatogenesis (OR = 0.03 (0.002-0.42); p = 0.01). A comparison of cTESE and microTESE revealed that the overall successful sperm retrieval rate was not significantly different between the two methods (74.1% vs. 58.6, p = 0.22). This study demonstrated that lower levels of FSH, LH and prolactin, and a higher testicular volume and better histopathology were associated with a higher sperm retrieval rate in the univariate analysis. In the multivariable analysis, only Sertoli cell-only syndrome appeared to have a significantly negative effect on the successful harvesting of sperm when compared with normal spermatogenesis.
Development of a new recurrence-free survival prediction nomogram for patients with primary non-muscle-invasive bladder cancer based on preoperative controlling nutritional status score
Authors: Zhao Liwei , Huang Jiaguo , Sun Ji , Wang Kai , Tai Shengcheng , Hua Runmiao , Yu Yufu , Fan Yi 
Published: June 2021

Abstract
Bladder cancer is the second most common neoplasm in the urogenital system in terms of morbidity and mortality, and there is an urgent need for a more accurate assessment of the individual prognosis in patients with primary non-muscle-invasive bladder cancer (NMIBC). The Controlling Nutritional Status (CONUT) score is an emerging biomarker score, which has been confirmed to have prognostic value in various malignant tumors. A total of 94 patients with NMIBC were analyzed retrospectively between January 2011 and December 2015.The Kaplan-Meier method was used to assess recurrence-free survival (RFS), and log-rank tests was used to test the equivalences of survival curves. We used univariate and multivariate Cox proportional hazards regression model to identify important predictors of RFS. In univariate analysis, age, history of smoking, pathological T stage, tumor grade, tumor size, and CONUT score were significantly correlated with RFS. Multivariate analysis indicated that CONUT score(HR = 3.855, 95% CI 1.242–11.970, p = 0.020) was an independent predictor of RFS in patients with NMIBC. Based on significant parameters in multivariate analysis and reliable recurrence predictors determined in predictive models and relevant guidelines, a new age-, history of smoking-, pathologic factors- and the CONUT score-based scoring model was developed to predict recurrence of NMBIC. In addition, we internally validated the nomogram using the consistency index and calibration plots, which showed the model has high prediction accuracy(c-index = 0.851). The development of a new nomogram based on CONUT score could increase the accuracy of recurrence prediction and improve individualized treatment plan for patients with NMIBC.
Mechanisms Involved in the Anti-Tumor Effects of Toosendanin in Glioma Cells
Authors: huang haiyan , Zhang Chaochao , Gao Haijun , Liu Ziqiang , Lai Jiacheng , Zhan Zhixin , Chen Yong 
Published: June 2021

Abstract
Background: Toosendanin (TSN) is a triterpenoid compound mainly used as an ascaris repellant. Recent studies have shown that it possesses antitumor effects in many types of tumor cells. However, the effects of TSN on glioma cells have rarely been reported. Methods: Different assays were performed to investigate the effects of TSN on the different glioma cell lines including U87MG and LN18. The assays included colony formation, wound healing, and transwell assays. Furthermore, Hoechst 3342 staining, flow cytometry, and western blotting analysis were performed to investigate the apoptotic activities of TSN. Finally, the results were confirmed using a xenograft tumor model that comprised of nude mice. Results: In vitro, the CCK-8 and colony formation assays showed that TSN effectively inhibited glioma cell proliferation. Moreover, the inhibitory effects on glioma cell migration and invasion were demonstrated through the wound healing and transwell assays, respectively. Hoechst 33342 staining, flow cytometry, and western blotting assays demonstrated the significant effect of TSN in the apoptosis induction of glioma cells. Furthermore, the anti-glioma effect of TSN was exerted through the inhibition of the PI3K/Akt/mTOR signaling pathways as demonstrated by western blotting analysis. In addition, the effects of TSN on glioma cell viability, apoptosis, cell cycle arrest, migration, and invasion were reversed by 740Y-P, a PI3K activator. Finally, the mouse xenograft model confirmed the suppressive effect of TSN on tumor growth in vivo. Conclusion: Our results suggest that TSN is a promising chemotherapeutic drug for patients with glioma.
Different prognostic effect of surgery, radiation and chemotherapy in pediatric medulloblastoma less and over three years old: A Surveillance, Epidemiology, and End Results (SEER) analysis
Authors: Deng Zhong , Wang Yichang , Li Xixi , Tang Hongxing , Yang Jia , Wang Tuo 
Published: June 2021

Abstract
Introduction

To clarify the prognostic value of extent of surgical resection, radiation and chemotherapy in pediatric medulloblastoma patients < 3 years old and ≥ 3 years old.
Methods

We used the Surveillance, Epidemiology, and End Results program to identify 1,495 pediatric patients diagnosed between 1973 and 2016 with medulloblastoma. Patients with incomplete or unknown clinical information were excluded. Basic characteristics between patients < 3 years old and ≥ 3 years old were compared. Then, we used Cox regression to investigate the impact of extent of surgical resection, radiation and chemotherapy on patient outcome.
Results

Gross total resection only significantly improved patient outcome in those ≥ 3 years old, and radiation independently correlated to better OS and PFS in patients ≥ 3 years old (all p < 0.05). However, chemotherapy only benefited patient outcome in those < 3 years old (all p < 0.05). Furthermore, in those ≥ 3 years old patients underwent surgical procedures, radiation alone confer survival benefit only in those received gross total resection (p < 0.05) but not in partial resection or biopsy (p > 0.05). Notably, adjuvant radiation plus chemotherapy largely improved patient survival independent of extent of resection (p < 0.05).
Conclusions

The extent of resection should be differentially considered and applied between pediatric medulloblastoma patients < 3 years old and ≥ 3 years old, so are the adjuvant radio- and chemo- therapies.