bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the patients’ perception on dental treatment and their anxiety levels during the COVID-19 pandemic process
Authors: KARAGÖZOĞLU İrem , PARLAR ÖZ Özge 
Published: September 2021

Aim: The purpose of this study was to evaluate the anxiety levels of patients against dental treatment during the pandemic process and to get some information about the level of awareness, perceptions and attitudes of patients applying for dental treatment. Also to determine the relationship between anxiety levels and sociodemographic variables of individuals.
Materials and Methods: 300 patients who applied to the dentistry faculty were included in this study and a questionnaire form was applied to these patients. The survey consist of three parts; in the first part patients' sociodemographic variables were asked, secondly State-Trait Anxiety Inventory-STAI developed by Spielberger was used to measure the level of anxiety and in the last part a questionnaire form about the perceived sensitivity to Covid-19 developed by the authors was applied. Statistical analysis was performed with SPSS for Windows version 24.0 and a P value < 0.05 was accepted as statistically significant. The normality of distribution of continuous variables was tested by Shaphiro Wilk test. Mann whitney u test for and Kruskal Wallis test and Dunn multiple comparison tests were for used to compare non-normal data across 2 or more than 2 groups.
Results: A total of 300 patients, 155 men and 145 women, participated in the study. The mean state anxiety score was 43.38 ± 8.34. Significant differences were not found among the independent variables affecting the level of anxiety, age, gender, marital status, education, whether individuals have had Covid-19. When the questions evaluating the patients perception on dental treatment were compared with the anxiety scores, statistically significant differences were found.
Conclusion: Although dentistry has the highest risk of contamination, it has been determined that dentists' care with protective equipment does not have a problem for patients. It has been determined that the anxiety level of the patients is high in places such as dental hospitals where many patients are treated, but they are aware of the preventive measures to be taken against the pandemic process.
Surgical Site Infection Post Caesarean Section: A Case Report
Authors: Hartati  , Febi Stevi Aryani  , M Satria Erlangga Sinum  , Emasrissa Murwani  
Published: September 2021

Background. Surgical site infection (SSI) is a major complication of surgery which can increase morbidity, mortality and cost of hospitality. Incidence of SSI at a healthcare provider/facility reflected a service quality of that institution.
Case presentation. A 28-years-old woman, a housewife, P2A1, post-caesarean section 12 days ago. Pasien datang dengan keluhan utama badan lemas dan terdapat nanah pada luka bekas operasi. Ia mengeluh perutnya terasa semakin panas, dan keluar cairan bening sebelum muncul nanah pada luka operasi. Pasien juga mengeluh demam, mual dan lemas akhir-akhir ini Laboratory investigations showed Hb 11,9 g/dL, Leukosit 11.100 m/L, trombosit 601.000. MCV 70,7 fL MCH 24 pg MCHC 32 g/dL TIBC 392 mg/dL serum Iron 54 mg/dL Ferritin 19,8 ng/mL, CRP reactive. Patient was wound dressing and given levofloxacin 500mg three times a day intravascular for three days and continue with ciprofloxacin three time a day oral. Patient was suggest to repeat laboratory test after therapy to evaluate therapy response.
Conclusion: The result showed that time of prophylactic antibiotics can used to minimize the occurrence of SSI in post-caesarean section patients.
Pelatihan Dasar Preventif Maintenance 1 Untuk Masyarakat Kecamatan Cipayung, Depok
Authors: Rahayu Minto , Apriana Asep , Abdillah Aziz 
Published: September 2021

Pemda Depok mempunyai Tempat Pengolahan Sampah (TPS) di Kecamatan Cipayung. Selama ini masyarakat Cipayung hanya menjadi penonton dan menerima dampak negatif dari keberadaan TPS, tanpa dapat berkontribusi dan mendapatkan keuntungan keekonomian. Progran Studi Alat Berat mempunyai tanggung jawab terhadap pemberdayaan masyarakat sehingga bermaksud melaksanakan pengabdian kepada masyarakat sesuai dengan kompetensi, yaitu Pelatihan dasar Preventive Mantenance 1 (PM 1) pada masyarakat Cipayung, Depok. Hasilnya masyarakat mempunyai keterampilan preventive maintenance, khususnya pada mesin pengolah sampah sehingga masyarakat dapat menjadi mitra Pemda Depok dalam hal pelayanan perawatan berkala terhadap unit alat berat.
Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water
Authors: SELWET MAREK 
Published: September 2021

Abstract
The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate Campylobacter spp. from water. The strains used in this research were isolated from water contaminated with sewage. Campylobacter coli alone was detected in the samples and used for further research. The reference strain C. coli ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of C. coli decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences (p < 0.05) in the number of C. coli cells, the cell death rate remained at the same level.
Loyalty as Affecting Mediator of Service Quality And Customer Satisfaction Towards Competitive Advantage
Authors: Sultoni Mohammad Hamim , Sudarmiatin Sudarmiatin 
Published: September 2021

When preparing an effective marketing strategy, a bank must consider its competitors as well as its customers. Establishing a mutual relationship with customers requires a better means of satisfying customer needs than the competitors. Micro, Small, and Medium-sized Enterprises (MSMEs) should always analyze their competitors and develop marketing strategies that effectively give the company a good position compared to the competitors and provide a competitive advantage as strong as possible. This research was conducted using a quantitative method. The design referred to causal relationship research. The data were then analyzed using the path analysis. Findings showed that customer satisfaction, service quality, and customer loyalty affected the competitive advantage of Sentra Batik Pamekasan. The effect of customer loyalty on the competitive advantage could be viewed from the number of Sentra Batik Pamekasan's customers who decided not to move to other financial institutions (banks). When the company could give better service quality, the customer loyalty and the competitive advantage among financial institutions would get higher.

Abstrak
Dalam mempersiapkan strategi pemasaran yang efektif, pihak perbankan harus mempertimbangkan pesaingnya dan juga konsumennya. Membangun hubungan antara konsumen yang menguntungkan, memerlukan alat pemuasan kebutuhan konsumen sebagai sasaran yang lebih baik daripada pesaingnya. UMKM harus senantiasa menganalisis pesaing dan mengembangkan strategi pemasaran kompetitif yang secara efektif memposisikan UMKM dalam menghadapi pesaing dan memberikan keunggulan kompetitif sekuat mungkin. Penelitian ini menggunakan metode kuantitatif, jenis penelitian termasuk kategori hubungan kausal sedangkan analisis dalam penelitian ini menggunakan analisis jalur (path analysis). Hasil penelitian menunjukkan bahwa kepuasan konsumen, kualitas layanan, dan loyalitas konsumen mempengaruhi keunggulan bersaing di Sentra Batik Pamekasasan. Dampak dari loyalitas konsumen terhadap keunggulan bersaing dapat dilihat dari seberapa banyak konsumen Sentra Batik Pamekasasan yang tidak pindah ke lembaga keuangan lain. Semakin bagus kualitas layanan maka semakin tinggi tingkat loyalitas konsumen dan keunggulan bersaing antar lembaga keuangan juga akan semakin tinggi.
Kata Kunci : Loyalitas konsumen, kepuasan konsumen, kualitas layanan, keunggulan bersaing
MANAGEMENT SYSTEM OF PESANTREN SAFETY OFFICER FOR LEARNING ORDER
Authors: Hakim Luqmanul 
Published: September 2021

The purpose of this study was to determine the implementation of the learning control system in Pondok Pesantren Darussalam Sumbersari Kediri. The orderliness of students' learning can be improved through the boarding school's security management system. This control system relies on the maximum role of Islamic boarding school security staff in the activities and activities that exist in Islamic boarding schools. This study uses a qualitative field method. This research is intended to understand the phenomenon of what is experienced by research subjects, for example behavior, perception, motivation, action, and others related to orderliness of learning in Darussalam Islamic Boarding School and security management systems and security strategies in improving learning order. This study concludes that the system for controlling learning activities at Pondok Pesantren Darussalam Sumbersari Kediri uses a punishment strategy that has value in learning. This punishment is termed takzir.
Keywords: safety officer, pesantren, learning order, ta'zir.
SISTEM PENGELOLAAN PETUGAS KEAMANAN PESANTREN DALAM KETERTIBAN PEMBELAJARAN
Abstrak
Tujuan penelitian ini adalah untuk mengetahui implementasi sistem penertiban pembelajaran di Pondok Pesantren Darussalam Sumbersari Kediri. Ketertiban pembelajaran santri dapat ditingkatkan melalui sistem pengelolaan keamanan pondok pesantren. Sistem penertiban ini bertumpu pada peran maksimal staf keamanan pondok pesantren dalam kegiatan dan aktifitas yang ada dipondok pesantren. Penelitian ini menggunakan metode kualitatif lapangan. Penelitian ini dimaksudkan untuk memahami fenomena tentang apa yang dialami oleh subjek penelitian misalnnya perilaku, persepsi, motivasi, tindakan, dan lain-lain berkaitan dengan ketertiban pembelajaran di Pondok Pesantren Darussalam dan sistem pengelolaan keamanan dan strategi keamanan dalam meningkatkan ketertiban pembelajaran. Penelitian ini menyimpulkan bahwa system penertiban kegiatan pembelajaran di Pondok Pesantren Darussalam Sumbersari Kediri menggunakan strategi punishment (hukuman) yang bernilai pembelajaran. Hukuman ini diistilahkan dengan takzir.
Kata kunci: petugas keamanan, pondok pesantren, ketertiban pembelaran, takzir.
HepParser: An Intelligent Software Program for Deciphering Low-Molecular-Weight Heparin Based on Mass Spectrometry
Authors: Wang Hui , Wang Yu , Hou Meijie , Zhang Chunming , Wang Yaojun , Guo Zhendong , Bu Dongbo , Li Yan , Huang Chuncui , Sun Shiwei 
Published: September 2021

Low-molecular-weight heparins (LMWHs) are considered to be the most successful carbohydrate-based drugs because of their wide use as anticoagulants in clinics. The efficacy of anticoagulants made by LMWHs mainly depends on the components and structures of LMWHs. Therefore, deciphering the components and identifying the structures of LMWHs are critical to developing high-efficiency anticoagulants. However, most LMWHs are mixtures of linear polysaccharides which are comprised of several disaccharide repeating units with high similarity, making it extremely challenging to separate and decipher each component in LMWHs. Here, we present a new algorithm named hepParser to decipher the main components of LMWHs automatically and precisely based on the liquid chromatography/mass spectrometry (LC/MS) data. When tested on the general LMWH using hepParser, profiling of the oligosaccharides with different degrees of polymerization (dp’s) was completed with high accuracy within 1 minute. When compared with the results of GlycReSoft on heparan sulfate samples, hepParser achieved more comprehensive and reasonable results automatically.
Hydrogeochemistry and Acidic Property of Submarine Groundwater Discharge Around Two Coral Islands in the Northern South China Sea
Authors: Lui Hon-Kit , Liu Min-Yun , Lin Hsiu-Chin , Tseng Hsiao-Chun , Liu Li-Lian , Wang Feng-Yu , Hou Wei-Ping , Chang Rae , Chen Chen-Tung Arthur 
Published: September 2021

Submarine groundwater discharge (SGD) is an important source of nutrients in many coastal regions, yet little information is available on its carbonate chemistry and controlling factors. This study examined the processes and factors controlling the hydrogeochemistry and acidic property of the groundwaters and SGD waters of two isolated coral islands, Liuqiu Island (13 km off southwestern Taiwan) and Dongsha Island (located in the northern South China Sea, 420 km away from Liuqiu Island). Our results showed that the total alkalinity and dissolved inorganic carbon (DIC) of the fresh SGD waters were controlled mainly by the chemical weathering of carbonate minerals. Part of the DIC came from the organic matter decomposition or soil CO2, reducing the pH and CO32− concentration. Distributions of the carbonate chemistry and nutrients of the SGD waters were controlled mainly by physical mixing between the groundwater and the ambient seawater under the seabed, the so-called subterranean estuary. The Ca2+ released through weathering significantly increased the saturation state of aragonite or calcite, reducing the corrosiveness of the SGD waters on the carbonate rocks. This study is likely the first to examine the effects of the acidic property of SGD waters on the biogenic carbonate spine of a sea urchin and a pteropod shell. The spring water with similar carbonate chemistry to that of the freshwater SGD endmember from Liuqiu Island with a saturation state of aragonite of 0.96 caused observable dissolution on the spine of a sea urchin and a pteropod shell, but the spine dissolved more readily. This was because the spine is made of high-Mg calcite, which has higher solubility than that of aragonite or calcite. Such a result implies that some marine organisms with carbonate skeletons or shells containing high Mg:Ca ratios may suffer the impact of ocean acidification earlier. Although the SGD may contribute less than 10% of freshwater discharge by rivers to the coastal area, its impact on coastal biogeochemical cycles and ecosystems due to its acidic property and continual effect on the coast all year round deserves further investigation.
Molecular Genetics, Clinical Characteristics, and Treatment Outcomes of KATP-Channel Neonatal Diabetes Mellitus in Vietnam National Children’s Hospital
Authors: Ngoc Can Thi Bich , Dien Tran Minh , De Franco Elisa , Ellard Sian , Houghton Jayne A. L. , Lan Nguyen Ngoc , Thao Bui Phuong , Khanh Nguyen Ngoc , Flanagan Sarah E. , Craig Maria E. , Dung Vu Chi 
Published: September 2021

BackgroundNeonatal diabetes mellitus (NDM) is defined as insulin-requiring persistent hyperglycemia occurring within the first 6 months of life, which can result from mutations in at least 25 different genes. Activating heterozygous mutations in genes encoding either of the subunits of the ATP-sensitive K+ channel (KATP channel; KCNJ11 or ABCC8) of the pancreatic beta cell are the most common cause of permanent NDM and the second most common cause of transient NDM. Patients with NDM caused by KATP channel mutations are sensitive to sulfonylurea (SU) treatment; therefore, their clinical management can be improved by replacing insulin with oral agents.Patients and MethodsSeventy patients were diagnosed with NDM between May 2008 and May 2021 at Vietnam National Children’s Hospital, and molecular genetic testing for all genes known to cause NDM was performed at the Exeter Genomic Laboratory, UK. Patients with ABCC8 or KCNJ11 mutations were transferred from insulin to oral SU. Clinical characteristics, molecular genetics, and annual data relating to glycemic control, SU dose, severe hypoglycemia, and side effects were collected. The main outcomes of interest were SU dose, SU failure (defined as permanent reintroduction of daily insulin), and glycemic control (HbA1c).ResultsFifty-four of 70 patients (77%) with NDM harbored a genetic mutation and of these; 27 (50%) had activating heterozygous mutations in ABCC8 or KCNJ11. A total of 21 pathogenic mutations were identified in the 27 patients, including 13 mutations in ABCC8 and 8 mutations in KCNJ11. Overall, 51% had low birth weight (below 3rd percentile), 23 (85%) were diagnosed before 3 months of age, and 23 (85%) presented with diabetic ketoacidosis. At diagnosis, clinical and biochemical findings (mean ± SD) were pH 7.16 ± 0.16; HCO3−, 7.9 ± 7.4 mmol/L; BE, −17.9 ± 9.1 mmol/L; HbA1C, 7.98% ± 2.93%; blood glucose, 36.2 ± 12.3 mmol/L; and C-peptide median, 0.09 (range, 0–1.61 nmol/l). Twenty-six patients were successfully transferred from insulin to SU therapy. In the remaining case, remission of diabetes occurred prior to transfer. Glycemic control on SU treatment was better than on insulin treatment: HbA1c and blood glucose level decreased from 7.58% ± 4.63% and 19.04 ± 14.09 mmol/L when treated with insulin to 5.8 ± 0.94% and 6.87 ± 3.46 mmol/L when treated with SU, respectively.ConclusionsThis is the first case series of NDM patients with ABCC8/KCNJ11 mutations reported in Vietnam. SU is safe in the short term for these patients and more effective than insulin therapy, consistent with all studies to date. This is relevant for populations where access to and cost of insulin are problematic, reinforcing the importance of genetic testing for NDM.
HLA-G1+ Expression in GGTA1KO Pigs Suppresses Human and Monkey Anti-Pig T, B and NK Cell Responses
Authors: Rao Joseph Sushil , Hosny Nora , Kumbha Ramesh , Naqvi Raza Ali , Singh Amar , Swanson Zachary , Levy Heather , Matson Anders W. , Steinhoff Magie , Forneris Nicole , Walters Eric , Hering Bernhard J. , Burlak Christopher 
Published: September 2021

The human leukocyte antigen G1 (HLA-G1), a non-classical class I major histocompatibility complex (MHC-I) protein, is a potent immunomodulatory molecule at the maternal/fetal interface and other environments to regulate the cellular immune response. We created GGTA1-/HLAG1+pigs to explore their use as organ and cell donors that may extend xenograft survival and function in both preclinical nonhuman primate (NHP) models and future clinical trials. In the present study, HLA-G1 was expressed from the porcine ROSA26 locus by homology directed repair (HDR) mediated knock-in (KI) with simultaneous deletion of α-1-3-galactotransferase gene (GGTA1; GTKO) using the clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas9) gene-editing system. GTKO/HLAG1+pigs showing immune inhibitory functions were generated through somatic cell nuclear transfer (SCNT). The presence of HLA-G1 at the ROSA26 locus and the deletion of GGTA1 were confirmed by next generation sequencing (NGS) and Sanger’s sequencing. Fibroblasts from piglets, biopsies from transplantable organs, and islets were positive for HLA-G1 expression by confocal microscopy, flow cytometry, or q-PCR. The expression of cell surface HLA-G1 molecule associated with endogenous β2-microglobulin (β2m) was confirmed by staining genetically engineered cells with fluorescently labeled recombinant ILT2 protein. Fibroblasts obtained from GTKO/HLAG1+pigs were shown to modulate the immune response by lowering IFN-γ production by T cells and proliferation of CD4+and CD8+T cells, B cells and natural killer (NK) cells, as well as by augmenting phosphorylation of Src homology region 2 domain-containing phosphatase-2 (SHP-2), which plays a central role in immune suppression. Islets isolated from GTKO/HLA-G1+genetically engineered pigs and transplanted into streptozotocin-diabetic nude mice restored normoglycemia, suggesting that the expression of HLA-G1 did not interfere with their ability to reverse diabetes. The findings presented here suggest that the HLA-G1+transgene can be stably expressed from the ROSA26 locus of non-fetal maternal tissue at the cell surface. By providing an immunomodulatory signal, expression of HLA-G1+may extend survival of porcine pancreatic islet and organ xenografts.
Current Regulatory Requirements for Biosimilars in Six Member Countries of BRICS-TM: Challenges and Opportunities
Authors: Rahalkar Hasumati , Sheppard Alan , Santos Gustavo Mendes Lima , Dasgupta Chitralekha , Perez-Tapia Sonia Mayra , Lopez-Morales Carlos A. , Salek Sam 
Published: September 2021

Background: The aim of the study was to identify, interpret, and compare the current perspectives of regulatory agencies in six member countries of BRICS-TM (Brazil, Russia, India, China, South Africa, Turkey, and Mexico) on the different criteria used for biosimilar development and marketing authorisation process.Methods: A semi-quantitative questionnaire was developed covering the organisation of agency, biosimilar development criteria and marketing authorisation process and sent to seven regulatory agencies covering the BRICS-TM countries. All data was kept anonymous and confidential. Data processing and analysis was carried out; descriptive statistics were used for quantitative data and content analysis was employed to generate themes for qualitative data.Results: Out of the seven regulatory agencies included in the study, six representatives provided the responses. The perspectives of these six regulatory agencies varied on a number of aspects relating to the review criteria for biosimilar development and licencing process. The most prevalent model for data assessment is the “full review” of a marketing authorisation application. There is lack of a standard approach across the agencies on sourcing of the reference biological product, in vivo toxicity studies and confirmatory clinical studies. Most agencies restrict interaction with biosimilar developers and any scientific advice is non-binding. The marketing authorisation approval depends on scientific assessment of the dossier, sample analysis and GMP certification. The agencies do not issue any public assessment report specifying the summary basis of biosimilar approval.Conclusion: Regulatory agencies across the six emerging economies are steadily improving the regulatory mechanism in the area of biosimilars. However, there remains scope for increasing the effectiveness and efficiency of the processes by encouraging open and transparent interaction with developers, adopting a flexible approach toward accepting advanced analytical data in lieu of clinical studies and enhancing regulatory reliance amongst agencies. This will help to simplify the new biosimilar development programmes and make them more cost-effective.