bluuebunny/crossref_metadata_2025_split huggingface dataset:
Title: Weight and waist-to-hip ratio change pattern during the first five years of survival: data from a longitudinal observational Chinese breast cancer cohort
Authors: Lei Yuan-Yuan , Ho Suzanne C. , Kwok Carol , Cheng Ashley , Cheung Ka Li , Lee Roselle , Mo Frankie , Yeo Winnie 
Published: March 2021

Abstract
Background: Body weight management was an important component in breast cancer survivorship care. The present study described the change patterns of body weight and waist-to-hip ratio (WHR) during the first five years of survival, and investigated potential factors associated with very substantial changes.Patients and methods: Based on a longitudinal cohort with 1462 Chinese women with breast cancer, anthropometric measurements including body weight, height, waist and hip circumferences were measured by trained interviewers following standard protocol at four time-points: baseline at study entry, 18-, 36- and 60-months follow up assessments (termed as T0, T1, T2 and T3, respectively). Body height was measured at baseline and body weight at cancer diagnosis were retrieved from medical record.Results: Compared to weight at breast cancer diagnosis, the median weight change was -0.5kg, 0kg, +0.5kg, and +1kg at T0, T1, T2 and T3, respectively. During the first five years of survival, the proportion of women who were obese have slightly increased. At 60-months after diagnosis, only 14.3% of women had weight gain by > 5kg; and the percentage of women who had weight gain by > 10% was 10.7%. Nearly half of patients had abdominal obesity at study entry, and this proportion were gradually increased to nearly 70% at 60-months follow-up. Multivariate analysis indicated that older age, frequent sports participation and having vegetables and fruits intake ≥ 400g/day were related to lower risk of very substantial weight gain (> 10%) at 60-months follow-up; patients aged 40-49 years, having ≥ 2 comorbidities, ER negative and having vegetables and fruits intake ≥ 400g/day were associated with less likelihood of very substantial WHR substantial increase (> 10%) at 60-months follow-up. Conclusion: ﻿Weight gain was modest in Chinese breast cancer survivors during the first five years of survival, while central adiposity has become a contemporary public health issue. The incorporation of healthy weight and abdominal circumference patient education and management has a potential to improve cancer survivorship.
Biomass, Carbon Stock and Sequestration Potential of Oxytenanthera abyssinica forests in Lower Beles River Basin, Northwestern Ethiopia
Authors: Abebe Shiferaw , Minale Amare Sewnet , Teketay Demel , Jayaraman Durai 
Published: March 2021

Abstract
Background: Ethiopia is endowed with abundant bamboo resources. However, little is known about biomass, carbon sequestration and storage potential of bamboos. Therefore, this study was conducted to estimate the carbon sequestration and storage potential of Oxytenanthera abyssinica forests in Lower Beles River Basin, Northwestern Ethiopia. To this end, A total of 54 circular plots of 100 m2, with plots having a radius of 5.64, were established to conduct the inventory in Assitsa and Eddida bamboo forests, the typical bamboo sites in Lower Beles River Basin. Biomass accumulation of bamboo was estimated using an allometric equation based on diameter at breast height (DBH) and age. Soil samples were taken from two different soil depths (0 – 15 and 15 – 30 cm) to determine soil organic carbon.Results: Results indicate that a total of 1,620 bamboo culms were measured in the bamboo forests. The mean biomass of the bamboo forests in the study area accounted for about 140.11 ± 2.55 Mg ha-1. The mean biomass carbon and soil organic carbon stock of the bamboo forests were 65. 85 ± 1.19 and 69.70 ± 1.83 Mg C ha-1, respectively. Therefore, the mean carbon stock of the O. abyssinica bamboo forests was 135.63 ± 2.33 Mg C ha-1 with 497.8 ± 8.55 CO2 equivalents. Conclusion: Generally, the O. abyssinica bamboo forests of the study area have significant role in climate change mitigation. Therefore, sustainable management of these crucial vegetation resources will enhance their role in carbon sequestration and thereby, climate change mitigation.
Effective Prediction of Rheumatoid Arthritis (Ra) Diagnosis Using Hybrid Harmony Search with Adaptive Neuro Fuzzy Inference System
Authors: SHANMUGAM S , Saravanabhavan C , Arunkumar T 
Published: March 2021

Abstract
One of the severe auto immune diseases that affects the entire human body is Rheumatoid Arthritis (RA), the disease triggers one’s immune system to attack the inner linings of bones and causes severe inflammation of the synovium. The continuous erosion of bone lining leads to permanent loss of the joint, accounting this severity the early prognosis of the disease is a significant and inevitable process. But, the sign and symptoms of the disease are always uncertain. The symptom of RA disease is similar to other inflammatory diseases, so highly experienced experts can identify the disease in its early stage. To support the clinicians and technicians for early prognosis of the disease, a computer-aided decision support model based on Harmony Search –Adaptive Neuro Fuzzy Inference System is presented in this study. The Harmony search algorithm is employed to select the optimal features, and ANFIS is adopted to perform classification. To demonstrate the effectiveness of the model, metrics such as Accuracy, Sensitivity, Specificity, Precision, Recall, F-measure, Positive Predictive Value, Negative Predictive Value, Root Mean Square Error, and Mean Absolute Error are employed and evaluated in MATLAB simulation environment. The proposed HS-ANFIS outperformed other models developed in this research and existing works of literature.
Differences in Axial Length and IOLs Power Basing on Alternative A-Scan or Fellow-Eye Biometry in Macular-Off Rhegmatogenous Retinal Detachment Eyes
Authors: Liu Rui , Li Hongrong , Li Qingchen 
Published: March 2021

Abstract
We observe the potential refractive error basing on alternative A-scan ultrasound and fellow-eye biometry for phacovitrectomy in phakic macula-off rhegmatogenous retinal detachment (RRD) eyes, when IOLMaster fails to obtain data. Vitrectomy without lens extraction was performed for RRD repair. Preoperative axial length was measured using alternative A-scan ultrasound (AL-US). Postoperative axial length in eyes with silicone oil tamponade (AL-SO) and fellow-eye biometry (AL-FE) were obtained using IOLMaster. AL-US, AL-FE and AL-SO were 25.39 ± 2.14 mm, 25.85 ± 2.16 mm and 26.08 ± 2.53 mm, respectively. The Bland-Altman agreements among AL-US, AL-FE and AL-SO were well (95.5%, 21/22 of cases were in LoA). The mean IOLs power calculated using AL-US (Power-US) was 16.81 ± 7.19 D, using AL-FE (Power-FE) was 14.74 ± 6.95 D, and using AL-SO (Power-SO) was 13.54 ± 8.32 D. The difference between AL-US and AL-SO was significant (P < 0.05), while that between AL-FE and AL-SO was not (P > 0.05). The difference between Power-US and Power-SO was significant (P < 0.05), while that between Power-FE and Power-SO was not (P > 0.05). It showed that the alternative A-scan ultrasound leads to significant differences in axial length and IOLs power prediction, while fellow-eye biometry provided similar measurements compared with those of silicone oil-filled eyes after RRD repair.
A Head-to-Head Comparison of 68Ga-DOTA-FAPI-04 and 18F-FDG PET/MR in Patients with Nasopharyngeal Carcinoma: A Prospective Study
Authors: Qin Chunxia , Liu Fang , Huang Jing , Ruan Weiwei , Liu Qingyao , Gai Yongkang , Hu Fan , Jiang Dawei , Hu Yu , Yang Kunyu , Lan Xiaoli 
Published: March 2021

Abstract
PurposeTo conduct a head-to-head comparison of the diagnostic ability of 68Ga-DOTA-FAPI-04 (68Ga-FAPI) and 18F-FDG PET/MR in nasopharyngeal carcinoma (NPC) patients.MethodsPatients diagnosed with NPC were prospectively enrolled. All patients underwent head-and-neck 68Ga-FAPI PET/MR and 18F-FDG PET/MR within one week. Primary tumor, lymph node numbers, and tracer uptake were compared by SUVmax and visual evaluation. The primary tumor volumes derived from 68Ga-FAPI, 18F-FDG PET, and MRI were also compared.ResultsFifteen patients were enrolled from June to August 2020. Both 68Ga-FAPI and 18F-FDG PET had 100% detection rate of the primary tumor. The 68Ga-FAPI SUVmax of primary tumors (13.87±5.13) was lower than that of 18F-FDG (17.73±6.84), but the difference was not significant (p=0.078). Compared with 18F-FDG, 68Ga-FAPI PET improved the delineation of skull-base invasion in eight out of eight patients and intracranial invasion in four out of four patients. When 25%SUVmax of 68Ga-FAPI or 20%SUVmax of 18F-FDG was utilized as a threshold for determining tumor volume, it was highly consistent with MRI. 18F-FDG PET detected much more positive lymph nodes than 68Ga-FAPI (100 vs 48). The SUVmax of 48 paired lymph nodes was significantly lower on 68Ga-FAPI than 18F-FDG (8.67±3.88 vs 11.79±6.17, p<0.001). Additionally, 68Ga-FAPI further detected four highly suspected small, distant metastases in three patients. Compared with 18F-FDG, 68Ga-FAPI changed overall staging in six of fifteen patients, with three patients being up-staged, and three down-staged.Conclusion68Ga-FAPI outperforms 18F-FDG in delineating the primary tumor and detecting suspected distant metastases, particularly in the evaluation of skull-base and intracranial invasion, suggesting 68Ga-FAPI hybrid PET/MR has the potential to serve as a single-step staging modality for patients with NPC. However, its value regarding lymph node and distant metastases evaluation needs further study.Trial registration: NCT04554719. Registered September 8, 2020 - retrospectively registered, http://clinicaltrails.gov/show/ NCT04554719
Laparoscopic Caudal Approach of the Inferior Vena Cava for Isolated Segment 1 Liver Resection.
Authors: Tur-Martínez Jaume , Herrero-Fonollosa Èric , García-Domingo María Isabel , Camps-Lasa Judith , Sobrerroca-Porras Laura , Costa-Henere Daniel , Rodríguez Aurora , Cugat-Andorrà Esteban 
Published: March 2021

Abstract
Introduction:Isolated segment 1 laparoscopic liver resection is a very challenging procedure. Very few references are available about this laparoscopic technique, so the aim of this article is to show the main technical aspects of laparoscopic caudal approach for segment 1.Material and Methods: A 64 years old woman with a past medical history of a breast cancer previously operated (pT1N0M0, with positive hormonal receptors). Adjuvant treatment was done with radiotherapy and hormone-therapy (Tamoxifen). After 12 months of follow-up, a 18 mm single liver metastasis was detected in the segment 1, suggestive of metastatic disease. A complementary study was done with Magnetic Resonance Image, Computed Tomography and Positron Emission Tomography, without other lesions proven. Result:A laparoscopic resection of isolated liver segment 1 is performed with a caudal approach of the inferior vena cava. All the steps are carefully described. The surgery time was 120 minutes and the blood loss was less than 100 ml. No postoperative complications were registered. The patient was discharged on the third postoperative day.Conclusion:Liver 1 segment resection by laparoscopy with a caudal approach of the inferior vena cava is a secure technique in selected patients and it should be performed in experienced liver surgery and advanced laparoscopy centers, because of its high complexity.
Investigation of Malaria Vectors (Diptera: Culicidae) in Agricultural Settlements in the Amazon Region of Brazil
Authors: Andrade Alice Oliveira , Santos Najara Akira Costa dos , Castro Raphael Brum , Araujo Isabelle Sousa de , Bastos Alessandra da Silva , Magi Felipe Neves , Rodrigues Moreno Magalhães de Souza , Pereira Dhélio Batista , Medeiros Jansen Fernandes , Araujo Maisa da Silva 
Published: March 2021

Abstract
Background: Environmental changes resulting from the urbanization process represent a challenge for malaria control. The majority of malaria cases in South America occur in rural areas, areas of recent occupation, mining and indigenous areas of the Amazon region. Although these areas have a significant impact on malaria cases, few entomological studies have been carried out in areas of recent occupation. This study aimed to describe the density, natural infection rate and hematophagic behavior of anopheline species in two settlements in the state of Rondonia, Brazil in order to understand how malaria transmission occurs in areas that have been settled at different times.Methods: An area of recent occupation, denominated Acampamento Fortaleza (AF), and an old settlement, denominated Projeto de Assentamento Florestal Jequitibá (PAFJ), were studied. Peridomicile collections of anopheles were carried out using the Protected Human Attraction Technique (PHAT). The risk and potential for malaria transmission were assessed using the human biting rate (HBR), sporozoite rate (SR) and the entomological inoculation rate (EIR).Results: The results confirmed that Nyssorhynchus darlingi, the main vector responsible for the transmission of malaria in the state of Rondônia, is the predominant species in the two studied locations. Although settlement of the two study sites has occurred at different times, the species richness found was low, showing that the environmental changes caused by anthropological actions probably favor the adaptation of the Ny. darlingi species. Of the 615 anopheline mosquitoes assessed, 7 (1.1%) were positive for Plasmodium infections. The EIR revealed that Ny. darlingi contributes to the transmission of malaria in both locations, since it was responsible for 0.41 infectious bites in humans at night in PAFJ and 0.16 in AF. In the two study sites, the biting occurred more frequently at dusk.Conclusions: Ny. darlingi is the principal vector found in the studied locations. Its prevalence occurs in areas of recent colonization but, even when present in a low density, this species could maintain transmission of malaria in an older settlement. The entomological information obtained in this study is important and may aid the selection of vector control actions in these locations that are considered as having a high risk of malaria transmission.
Biofilm Persister Cell Formation, and Relative Gene Expression Analysis of Type II Toxin-Antitoxin System in Pseudomonas Aeruginosa Strains in the Exponential and Stationary Phases
Authors: Zadeh Rezvan Golmoradi , Kalani Behrooz Sadeghi , Ari Marzie Mahdizade , Talebi Malihe , Razavi Shabnam , Jazi Faramarz Masjedian 
Published: March 2021

Abstract
Chronic and persistent infections and therapy failure are concerning issues in patients with Pseudomonas aeruginosa infections. Presence of persister cells in biofilm considers as one from the causes for antibiotic resistance and treatment failure. Consequently, in this research, the expression of TA type II systems into persister formation of biofilm was assessed in presence of colistin ciprofloxacin antibiotics into exponential and stationary phases. The results showed that TA systems at different stages of bacterial growth in biofilm have different functions that subsequently, cause differences in the presence amount of persister cells at each stage of bacterial growth. The expression of the systems were measured by Real-Time PCR method. Keywords: Persister cell; Biofilm; Pseudomonas aeruginosa; TA systems; Real-time PCR; Exponential and Stationary phases
Characteristics and Adverse Outcomes of Chinese Adolescent Pregnancy Between 2012 and 2019: An Observational Study of 438 Health Facilities
Authors: Xie Yanxia , Wang Xiaodong , Mu Yi , Liu Zheng , Wang Yanping , Li Xiaohong , Dai Li , Li Qi , Li Mingrong , Chen Peiran , Zhu Jun , Liang Juan 
Published: March 2021

Abstract
We aimed to describes characteristic of adolescent pregnancy, determines its effect on adverse maternal and perinatal outcomes and explores whether that association varies with gestational age with the goal of proposing specific recommendations for adolescent health in China. 2,366,559 women aged 10-24 years who had singleton pregnancies between 2012 and 2019 from 438 hospitals were included in the study. Adolescent pregnancy was defined as younger than 20 years of age. We used multivariable logistic regression to estimate the effect. Women aged 20-24 years served as the reference group in all analyses. The proportion of rural girls with adolescent pregnancies rebounded after 2015 despite common-law marriage in rural areas have improved. Higher risks of eclampsia (adjusted odds ratio (aOR) 1.87, 95% confidence interval (CI) 1.57~2.23), severe anaemia (aOR 1.18, 95% CI 1.09~1.28), maternal near miss (aOR 1.24, 95% CI 1.12~1.37), and small-for-gestational-age (aOR 1.30, 95% CI 1.28~1.33) were observed when gestational age was > 37 weeks. Adolescent pregnancy was independently associated with increased risks of other perinatal outcomes. Further implementation of pregnancy prevention strategies and improved health care interventions are needed to reduce adolescent fertility and to avoid adverse fertility outcomes among adolescent women in China at a time when adolescent fertility is rebounding.
Establishment and Optimization of Molecular Cytogenetic Techniques (45S Rdna-FISH, GISH And Fiber-FISH) In Kiwifruit (Actinidia L.)
Authors: Zhao Yang , Deng Honghong , Chen Yao , Li Jihan , Chen Silei , Li Chunyan , Mu Xue , Hu Zhongrong , Li Kunming , Wang Weixing 
Published: March 2021

Abstract
Background: Kiwifruit has long been regarded as ‘the king of fruits’ for its nutritional importance. However, the molecular cytogenetics of kiwifruit has long been hampered because of the large number of basic chromosome (x=29), the inherent small size and highly similar morphology of metaphase chromosomes. Fluorescence in situ hybridization (FISH) is an indispensable molecular cytogenetic technique widely used in many plant species. Herein, the effects of post-hybridization washing temperature on FISH, blocking DNA concentration on genomic in situ hybridization (GISH), extraction method on nuclei isolation and the incubation time on the DNA fiber quality in kiwifruit were evaluated.Results: The post-hybridization washing in 2×SSC solution for 3×5 min at 37 ˚C ensured high stringency and distinct specific FISH signals in kiwifruit somatic chromosomes. The use of 50× blocking DNA provided an efficient and reliable means of discriminating between chromosomes derived from in the hybrids of A. chinensis var. chinensis (2n=2x=58) × A. eriantha Benth (2n=2x=58), and inferring the participation of parental genitors. The chopping method established in the present study were found to be very suitable for preparation of leaf nuclei in kiwifruit. A high-quality linear DNA fiber was achieved by an incubation of 20 min. The physical size of 45S rDNA signals was approximately 35-40 μmm revealed by the highly reproducible fiber-FISH procedures established and optimized in this study.Conclusions: The molecular cytogenetic techniques (45S rDNA-FISH, GISH, and high-resolution fiber-FISH) for kiwifruit was for the first time established and optimized in the present study, which is the foundation for the future genomic and evolutionary studies.
NCOA3 as a Critical Oncogene in Thyroid Cancer via Modulating Major Signaling Pathways
Authors: Li Yujun , Liang JunRong , Dang Hui , Chen Pu , Zhang Rui , Shao Yuan 
Published: March 2021

Abstract
Nuclear Receptor Coactivator (NCOA3) enhances transcriptional activation of nuclear hormone receptors. This study was designed to explore the role of NCOA3 in thyroid cancer. Our data demonstrated that protein expression of NCOA3 was significantly upregulated in thyroid cancer tissues. NCOA3 knockdown inhibited cell proliferation and invasion, and induced cell cycle arrest and apoptosis in thyroid cancer. Conversely, ectopic expression of NCOA3 promoted cell proliferation and invasiveness in thyroid cancer. Mechanically, NCOA3 promotes thyroid cancer cell survival and invasiveness through modulating PI3K/AKT, MAPK and Wnt/β-catenin pathways. Collectively, these findings suggest that NCOA3 is critical in the initiation and development of thyroid cancer, and maybe a possible marker for prognostic and therapeutic.
The Effect of Oxygen Mixing Percentage on Structural, Optical and Electrical Properties of ZnTiO3 Thin-films Grown by Rf Magnetron Sputtering.
Authors: H srinivasa varaprsad , V sridevi P. , M Satya Anuradha , Pattipaka Srinivas , D pamu 
Published: March 2021

Abstract
Perovskites are important composites in the area of multidisciplinary applications. It is achieved by carefully choosing and tuning the properties of the thin-film at the deposition. In this paper, ZnTiO3 (ZTO) thin-films were being deposited on quartz and N-Si substrates by RF magnetron sputtering. The thin-films were developed at room temperature, oxygen percentage levels varying from 0 to 100, and annealed at 600oC. The electrical, optical, morphological, and structural properties were analyzed as a function of oxygen mixing percentage (OMP). The crystallinity of the cubic structured ZTO thin-film is found to be high at 25 OMP, and it is gradually decreased with increased OMP. The surface morphology of the thin-film is observed, and roughness is measured from the atomic force microscope. Raman Spectroscopy investigated the phase formation and the vibrational modes of the thin-film with their spectral de-convolution. The ZTO thin-films optical properties were investigated using transmittance spectra. The ZTO thin-film indicated the highest refractive index of 2.46, at 633nm with optical bandgap values of 3.57 eV, with a thickness of 145nm and 25 OMP. The refractive index, thin-film thickness, and excitation coefficient were analyzed using the Swanepoel envelope technique. Electrical characteristics of ZTO thin-film are measured from the optimized conditions of the thin-film with conventional thermionic emission (TE) technique.
The Microenvironment Combining with Immune Infiltration of Uveal Melanoma
Authors: Zhang Daowei , Wu Jiawen , Wu Jihong , Zhang Shenghai 
Published: March 2021

Abstract
Background: Uveal melanoma (UM) is the most common intraocular malignancy in adults. Although immunotherapy provided novel options in the disease's progression, it only benefits a minority of patients. The understanding of the UM microenvironment and the potential therapeutic targets in the microenvironment is still undefined. We aimed to propose a novel classification of UM microenvironment to identify ideal biomarkers for prognosis and potential targets for effective immunotherapy.Methods: In this study, we obtained the gene expression profile of 80 UM patients from the Cancer Genome Atlas (TCGA) and calculated immune/stromal scores based on the Estimation of stromal and Immune cells in Malignant Tumours using Expression data (ESTIMATE) algorithm dividing patients into the high- and low-expression groups. Then, 1024 immune-related differently expressed genes (DEGs) were selected in total following the annotation and enrichment analysis and key genes were screened by PPI network. Consequently, we conducted CIBERSORT algorithm and TIMER database to analyses the correlation between key genes and immune cells as well as utilized GeneCards database to analyses the correlation between key genes and the disease. Additionally, GSVA was performed to enrich the nonparametric and unsupervised signaling pathways of key genes and the drug sensitivity of them were predicted.Results: Based on high- and low-expression groups, we found that there were 888 up-regulated DEGs and 126 down-regulated DEGs in total, which were mainly enriched in 4 pathways in GO and 3 pathways in KEGG. Combining with the 10 genes screened by PPI, KM-plot showed that B2M and HLA-B were significantly affected the survival of UM patients. Among 22 immune cells, B2M and HLA-B were mainly corelated with 11 of them. GSVA results revealed 42 pathways significantly enriched of B2M while 41 pathways enriched of HLA-B. Finally, we predicted the Paclitaxel as the hopefully treatment for HLA-B.Conclusions: Our study not only understands deeper of fundamental biological features of microenvironment but also with potential therapeutic targets of UM.