bluuebunny/crossref_metadata_2025_split huggingface dataset:
Viromics Unveils Extraordinary Genetic Diversity of the Family Closteroviridae in Wild Citrus
Authors: Liu Qiyan , Zhang Song , Mei Shiqiang , Zhou Yan , Zhou Changyong , Cao Mengji 
Published: January 2021

Abstract
Background

Our knowledge of citrus viruses is largely skewed toward virus pathology in cultivated orchards; comparatively little is known about the virus diversity and ecology in natural ecosystems. The metatranscriptomics approach were used to analyze wild citrus from the Ailao Mountain region in China, a region embracing huge biodiversity in an area where citrus species originated. We investigated the evolutionary history of citrus viruses to identify the factors facilitating outbreaks of emerging viruses.
Results

An independent wild citrus habitat were identified where citrus tristeza virus (CTV) exists along with three novel monopartite, graft-transmissible members of the family Closteroviridae differing from the managed agricultural systems that harbor other diverse citrus viruses. The viruses analyzed in 16 sequencing libraries of 32 citrus samples comprised five CTV genotypes, two inferred new ampelovirus species, and a monopartite ancestor type of the multipartite crinivirus that may represent a new taxon. Acquisition of heat shock protein genes (all viruses) and coat protein duplication (most), exchange of other exogenous genes (some), and alteration of expression strategy (rare) may reflect the significant historical nodes through which a Closteroviridae ancestor as the relic species has flourished. During viral expansion, genetic mutation coupled with recombination may be the essential impetus to update best-adapted sequences, as in the cases of our CTV and one of the ampeloviruses.
Conclusions

This research represents the first time of virus diversity in wild citrus. The findings deepen our knowledge of Closteroviridae modular evolution and diversification. Given the potential emergence of similar novel viruses as pathogens, the need for surveillance of their pathogenic and epidemiological characteristics and the presence of other novel citrus viruses among wild citrus is of utmost priority for global citrus production.
Mitochondria-targeting Graphene Oxide Nanocomposites for Fluorescence Imaging-guided Synergistic Phototherapy of Drug-resistant Osteosarcoma
Authors: Zeng Wei-Nan , Yu Qiu-Ping , Wang Duan , Liu Jun-Li , Yang Qing-Jun , Zhou Zong-Ke , zeng yiping 
Published: January 2021

Abstract
Background: Osteosarcoma (OS) is the most common primary malignant bone tumor occurring in children and young adults. Drug-resistant osteosarcoma often results in chemotherapy failure. Therefore, new treatments aimed at novel therapeutic targets are urgently needed for the treatment of Drug-resistant osteosarcoma. Mitochondria-targeted phototherapy, i.e., synergistic photodynamic/photothermal therapy, has emerged as a highly promising strategy for treating drug-resistant tumors. This study proposed a new nano-drug delivery system based on near-infrared imaging and multifunctional graphene, which can target mitochondria and show synergistic phototherapy, with preferential accumulation in tumors.Methods and Results: Based on our previous study, (4-carboxybutyl) triphenyl phosphonium bromide (TPP), a mitochondria-targeting ligand, was conjugated to indocyanine green (ICG)-loaded, polyethylenimine-modified PEGylated nanographene oxide sheets (TPP-PPG@ICG) to promote mitochondrial accumulation after cellular internalization. Thereafter, exposure to a single dose of near-infrared irradiation enabled synergistic photodynamic and photothermal therapy, which simultaneously inhibited adenosine triphosphate synthesis and mitochondrial function. Induction of intrinsic apoptosis assisted in surmounting drug resistance and caused tumor cell death. After fluorescence imaging-guided synergistic phototherapy, the mitochondria-targeting, multifunctional graphene-based, drug-delivery system showed highly selective anticancer efficiency in vitro and in vivo, resulting in marked inhibition of tumor progression without noticeable toxicity in mice bearing doxorubicin-resistant MG63 tumor cells. Conclusion: The mitochondria-targeting TPP-PPG@ICG nanocomposite constitutes a new class of nanomedicine for fluorescence imaging-guided synergistic phototherapy and shows promise for treating drug-resistant osteosarcoma.
Seropositivity and Risk Factors of Brucella Infection in Small Ruminants that had History of Recent Abortion in the Afar Region of North-Eastern Ethiopia
Authors: Tekle Muluken , Legesse Mengistu , Mamo Gezahegne 
Published: January 2021

Abstract
Background: Brucellosis is one of the most important reproductive disease causes abortion and breeding failure in small ruminants and also causes severe systemic diseases in exposed humans. In Ethiopia, several studies of seroprevalence shows the magnitude and distribution of brucellosis both in animals and humans vary in different geographical localities. However, except few studies in Ethiopia all these serological studies was limited to RBPT and CFT, so far not supplemented with a varities of serological tests like ELISA to detect brucella infection, which is increase the likehood of detecting infected individulas and also improve the reliability of epidemiological data for appropriate control strategies. Hence, the present study was conducted in Amibara district of Afar Region, Ethiopia to detect the seropositivity and risk factor of Brucella infection in small ruminants that had history of recent abortion using mRBPT, cELISA and CFT. Materials and methods: Sera were collected from 226 animals (195 goats and 31sheep) and assessed for seropositivity of Brucella infection using modified Rose Bengal Plate Test (mRBPT), Complement Fixation Test (CFT) and competitive Enzyme Linked Immuno Sorbent Assay (cELISA).Results : In this study the over all seroprevalence was 12.0% (27 out of 226), 7.5% (17 out of 226) and 26.6% (60 out of 226) by mRBPT, CFT and cELISA, respectively. Out of 27 sera which were reactive by mRBPT, 17 (63.0%) were also reactive by (CFT). Out of the 17 sera which were reactive by CFT and mRBPT, 14 (82.4%) were reactive by cELISA. Out of the 29 sera which were non-reactive both by mRBPT and CFT, 10 (34.5%) were found to be reactive by cELISA. Out of the 226 sera which were tested both by mRBPT and cELISA, 20 (8.9%) were reactive by both tests, while 159 (70.4%) were non-reactive by both tests. The percentage of test agreement (79.2%) between mRBPT and cELISA was poor (k= 0.353). A high seropositivity for Brucella infection was significantly associated with the presence of retained placenta in the study animals (adjusted OR= 2.2, 95%CI, 1.1-4.4, P=0.030) as detected by cELISA. Conclusion: The findings of this study could suggest that brucellosis is main cause of abortion and breeding failure in small ruminants that had histry of recent abortion in the pastoral communities’ andwarrants the need for proactive measures to reduce its economic impact and risk of zoonotic transmission. This study indicates that cELISA based seroepidemiological survey increase the likehood of detecting infected individulas of brucellosis and also would be useful to provide reliable evidence for Brucella infection in small ruminants compared to mRBPT.
Association Between Adult Attachment Styles and Disordered Eating Among a Sample of Lebanese Adults
Authors: Zakhour Maha , Haddad Chadia , Salameh Pascale , Sacre Hala , Hallit Rabih , Akel Marwan , Kheir Nelly , Hallit Souheil , Obeid Sahar 
Published: January 2021

Abstract
ObjectivesThe current study will be the first to investigate relationships between attachment styles and disordered eating (binge eating, restrained eating, emotional eating, orthorexia tendencies and behaviors, and eating attitudes) and to check if these associations are mediated by body dissatisfaction among Lebanese adults. Designcross-sectional study.Settingall Lebanese governorates.ParticipantsA total of 811 participants was randomly selected to participate in this five-month study (January to May 2018). ResultsHaving an anxious attachment style was significantly associated with higher emotional eating, whereas having an avoidant attachment style was significantly associated with more inappropriate eating (higher EAT-26 scores) and more orthorexia nervosa (lower ORTO-R scores). Finally, having a secure attachment style was significantly associated with more appropriate eating (lower EAT-26 scores). Higher body dissatisfaction was significantly associated with higher orthorexia nervosa (lower ORTO-R scores), higher binge and restrained eating. Body dissatisfaction partially mediated the association between having an anxious attachment style and restrained eating (17.25%), having a secure attachment style and emotional eating (12.53%), eating attitudes (7.12%) and binge eating (21.47%), as well as the association between having an avoidant attachment style and orthorexia nervosa (9.75%). ConclusionIn conclusion, this research showed that attachment insecurity affected the maintenance of disordered eating symptoms and that body dissatisfaction plays a mediator role between adult attachment and disordered eating. Clinicians may consider interviewing or self-reporting measures to assess attachment insecurity.
Understanding the benefits and burdens associated with a Malaria Human Infection Study in Kenya: Experiences of study volunteers and other stakeholders
Authors: Chi Primus Che , Owino Esther Awuor , Jao Irene , Olewe Fredrick , Ogutu Bernhards , Bejon Philip , Kapulu Melissa , Kamuya Dorcas , Marsh Vicki , Team The CHMI-SIKA Study 
Published: January 2021

Abstract
Background Human infection studies that involve deliberately infecting healthy volunteers with a pathogen raise important ethical issues, including the need to ensure that benefits and burdens are understood and appropriately accounted for. Building on earlier work, we embedded social science research within an ongoing malaria human infection study in coastal Kenya to understand the study benefits and burdens experienced by study stakeholders in this low-resource setting and assess the wider implications for future research planning and policy. Methods Data were collected using qualitative research methods, including in-depth interviews (44), focus group discussions (10) and non-participation observation. Study participants were purposively selected (key informant or maximal diversity sampling), including volunteers in the human infection study, study staff, community representatives and local administrative authorities. Data were collected during and up to 18 months following study residency, from sites in Coastal and Western Kenya. Voice recordings of interviews and discussions were transcribed, translated and analysed using Framework Analysis, combining data- and theory-driven perspectives.Findings Physical, psychological, economic and social forms of benefits and burdens were experienced across study stages. Important benefits for volunteers included the study compensation, access to health checks, good residential living conditions, new learning opportunities, developing friendships and satisfaction at contributing towards a new malaria vaccine. Burdens primarily affected study volunteers, including experiences of discomfort and ill-health; fear and anxiety around aspects of the trial process, particularly deliberate infection and the implications of prolonged residency; anxieties about early residency exit and interpersonal conflict. These issues had important implications for volunteers’ families, study staff and the research institution’s reputation more widely.Conclusion Developing ethically and scientifically strong HIS relies on grounded accounts of volunteers, study staff and the wider community, understood in the socioeconomic, political and cultural context where studies are implemented. Recognition of the diverse, and sometimes perverse, nature of potential benefits and burdens in a given context, and who this might implicate, is critical to this process. Prior and on-going stakeholder engagement is core to developing these insights, which can be importantly expanded and underlined through embedded forms of social science research.
The ancient Chinese civilization left remarkable signals in the marine environment since the Bronze Age
Authors: Sun Xiang , Hu Limin , Hu Bangqi , Sun Xueshi , Wu Xiao , Bi Naishuang , Lin Tian , Guo Zhigang , Yang Zuosheng 
Published: January 2021

Abstract
To understand the onset of human civilization evolution recorded in the marine environment, a 6000-year record of black carbon (BC), including char and soot, was examined in a sediment core from the central South Yellow Sea. The results showed that the colder and drier climate dominated the variation in fire activity in northern China after mid-Holocene via decreased precipitation and vegetation cover. The char released from the fire activity, can barely retrieve traces preserved in the marine sediments during the civilization evolution in China since ~1 ka BP. Fortunately, the soot-BC signal demonstrated that anthropogenic forces have overwhelmed the natural causes of soot emission since ~4 ka BP (Bronze Age) in northern China. The variation in soot closely matched periods when there was large-scale use of coal or charcoal after ~2 ka BP and when indigenous coking technology was promoted after ~1.3 ka BP, and times with low soot abundance coincided with periods of social unrest. This work provides evidence that the soot signal could be a robust tracer for tracking the civilization evolution, and the ancient Chinese civilization left remarkable soot signals in the marine environment since the Bronze Age.
Discovering the Low-Latitude Ionospheric Trough Associated With the Inner Radiation Belt
Authors: Karpachev Alexander 
Published: January 2021

Abstract
The dynamics of ionospheric troughs during great geomagnetic storm on April 11–13, 2001 is considered. An analysis is based on measurements of electron density at altitudes of the CHAMP satellite 410–465 km. The subauroral, mid-latitude and low-latitude troughs were observed at nighttime, sometimes simultaneously. The subauroral trough is usually defined as the main ionospheric trough. The mid-latitude trough is associated with the magnetospheric ring current. It appears at the beginning of the storm recovery phase at latitudes of 40–45° GMLat (L=1.7–2.0) and exists for a long time at the late recovery phase at latitudes of the residual ring current 50–55° GMLat (L~2.4–3.0). The low-latitude trough was revealed for the first time. It is developed at the latitudes of the inner radiation belt 34–45° GMLat (L=1.45–2.00). This trough is associated with the precipitation of energetic particles from the inner radiation belt.
Quantification of Arterial, Venous, and Cerebrospinal Fluid Flow Dynamics by Magnetic Resonance Imaging Under Simulated Micro-Gravity Conditions: A Prospective Cohort Study
Authors: Zahid Arslan , Martin Bryn , Collins Stephanie , Oshinski John N. , Ethier Christopher Ross 
Published: January 2021

Abstract
Background: Astronauts undergoing long-duration spaceflight are exposed to numerous health risks, including Spaceflight-Associated Neuro-Ocular Syndrome (SANS), a spectrum of ophthalmic changes that can result in permanent loss of visual acuity. The etiology of SANS is not well understood but is thought to involve changes in cerebrovascular flow dynamics in response to microgravity. There is a paucity of knowledge in this area; in particular, cerebrospinal fluid (CSF) flow dynamics have not been well characterized under microgravity conditions. Our study was designed to determine the effect of simulated microgravity (head-down tilt [HDT]) on cerebrovascular flow dynamics. We hypothesized that microgravity conditions simulated by acute HDT would result in increases in CSF pulsatile flow. Methods: In a prospective cohort study, we measured flow in major cerebral arteries, veins, and CSF spaces in fifteen healthy volunteers using phase contrast magnetic resonance (PCMR) before and during 15° HDT.Results: We found a decrease in all CSF flow variables [systolic peak flow (p=0.009), and peak-to-peak pulse amplitude (p=0.001)]. Cerebral arterial average flow (p=0.04), systolic peak flow (p=0.04), and peak-to-peak pulse amplitude (p=0.02) all also significantly decreased. We additionally found a decrease in average cerebral arterial flow (p=0.040). Finally, a significant increase in cerebral venous cross-sectional area under HDT (p=0.005) was also observed. Conclusions: These results collectively demonstrate that acute application of -15° HDT caused a reduction in CSF flow variables (systolic peak flow and peak-to-peak pulse amplitude) which, when coupled with a decrease in average cerebral arterial flow, systolic peak flow, and peak-to-peak pulse amplitude, is consistent with a decrease in cardiac-related pulsatile CSF flow. These results suggest that decreases in cerebral arterial inflow were the principal drivers of decreases in CSF pulsatile flow.
The oncometabolite R-2-hydroxyglutarate dysregulates the differentiation of human mesenchymal stromal cells via inducing DNA hypermethylation
Authors: Liu Lizhen , Hu Kaimin , Feng Jingjing , Wang Huafang , Fu Shan , Wang Binsheng , Wang Limengmeng , Xu Yulin , Yu Xiaohong , Huang He 
Published: January 2021

Abstract
Background: Isocitrate dehydrogenase (IDH1/2) gene mutations are the most frequently observed mutations in cartilaginous tumors. The mutant IDH causes elevation in the levels of R-enantiomer of 2-hydroxylglutarate (R-2HG). Mesenchymal stromal cells (MSCs) are reasonable precursor cell candidates of cartilaginous tumors. This study aimed to investigate the effect of oncometabolite R-2HG on MSCs. Methods: Human bone marrow MSCs treated with or without R-2HG at concentrations 0.1 to 1.5mM were used for experiments. Cell Counting Kit-8 was used to detect the proliferation of MSCs. To determine the effects of R-2HG on MSC differentiation, cells were cultured in osteogenic, chondrogenic and adipogenic medium. Specific staining approaches were performed and differentiation-related genes were quantified. Furthermore, DNA methylation status was explored by Illumina array-based arrays. Real-time PCR was applied to examine the signaling component mRNAs involved in. Results: R-2HG showed no influence on the proliferation of human MSCs. R-2HG blocked osteogenic differentiation, whereas promoted adipogenic differentiation of MSCs in a dose-dependent manner. R-2HG inhibited chondrogenic differentiation of MSCs, but increased the expression of genes related to chondrocyte hypertrophy in a lower concentration (1.0 mM). Moreover, R-2HG induced a pronounced DNA hypermethylation state of MSC. R-2HG also improved promotor methylation of lineage-specific genes during osteogenic and chondrogenic differentiation. In addition, R-2HG induced hypermethylation and decreased the mRNA levels of SHH, GLI1and GLI2, indicating Sonic Hedgehog (Shh) signaling inhibition. Conclusions: The oncometabolite R-2HG dysregulated the chondrogenic and osteogenic differentiation of MSCs possibly via induction of DNA hypermethylation, improving the role of R-2HG in cartilaginous tumor development.
Effectiveness of the school-based social and behaviour change communication interventions on insecticide-treated nets utilization among primary school children in rural Ethiopia: a controlled quasi-experimental design
Authors: Ababulgu Fira Abamecha , Sudhakar Morankar , Gebretsadik Lakew Abebe , Lemu Yohannes Kebede , Alemayehu Guda , Koricha Zewdie Birhanu 
Published: January 2021

Abstract
BackgroundSchool-based behaviour change communication interventions could help to achieve behavioural changes in the school and enhance the enrollment of the students and teachers as health messengers to local communities. Evidence on the impacts of the school-engaged malaria preventive interventions are limited as far as the social and behaviour change communication (SBCC) is concerned. This study examined the effectiveness of the school-based SBCC approach on insecticide-treated nets (ITNs) utilization among primary school students in malaria-endemic settings of Ethiopia.MethodsVarious participatory, educational, and communication interventions were implemented from 2017 to 2019 in 75 primary schools and respective villages in Jimma to promote malaria preventive practices. A quasi-experimental design was conducted with randomly selected 798 students (i.e. 399 intervention and 399 control groups). Data were collected by trained interviewers using structured questionnaires. The SPSS version 26 software was used to analyse the data. Propensity score matching analysis was performed to control for possible confounding biases. The average effects of the intervention were estimated using multivariate general linear modelling to estimate for mean differences and odds ratio based on the nature of data.ResultsThe result showed that the ITNs utilization was 6.857 folds in the intervention groups compared to the counterpart; (OR=6.857; 95% CI: (4.636, 10.1430); effect size=39%). A mean differences (MD) of self-efficacy (MD=15.34; 95% CI: 13.73 to 16.95), knowledge (MD=5.83; 95% CI: 5.12 to 6.55), attitude (MD=6.01; 95% CI: 5.26 to 6.77), perceived malaria risk (MD=2.14; 95% CI: 1.53 to 2.76), and perceived family supports (MD=6.39; 95% CI: 5.57 to 7.22) were observed favoring the intervention. Multivariable logistic regression modelling results showed that knowledge (β=0.194, 95% CI: 1.09 to 1.35) and perceived family supports (β=0.165, 95% CI: 1.11 to 1.25) and self-efficacy (β=0.10, 95% CI: 1.22 to 2.32) predicted the ITN utilization among the school children.ConclusionThe finding of this study suggested that the school-based SBCC approach combined with peer education activities advanced the malaria-related knowledge, attitude, self-efficacy, risk perceptions, and family supports and ultimately improved the sustained use of ITNs among school-going children. Further research should be conducted to understand the mechanism of these effects given the influences of social, health services, and school systems are considered.