bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model using Lipopolysaccharide
Authors: Lv Si-Ji , Hou Shu-Hui , Gan Lei , Sun Jing 
Published: June 2021

Abstract
Background: This study aimed to establish a lipopolysaccharide (LPS)-induced primary ovarian insufficiency (POI) mouse model and to investigate the underlying mechanism.Methods: C57BL/6N female mice were intraperitoneally injected with low-dose LPS (0.5 mg/kg) once daily for 14 days, high-dose LPS (2.5 mg/kg) twice weekly for 2 weeks, and cyclophosphamide (CTX; 150 mg/kg) once weekly for 2 weeks. Ovarian function was assessed by measuring the length of the estrous cycle, the number of primordial follicles, and the levels of serum pituitary/ovarian hormones. Expression and production of interleukin 1β (IL-1β) were determined to evaluate ovarian inflammation. Histopathological examination was performed to examine ovarian fibrosis. TUNEL assay was carried out to evaluate granulosa cell apoptosis. Western blotting was performed to measure the levels of inflammation-, fibrosis-, and apoptosis-related proteins in mouse ovaries.Results: Like CTX, both low- and high-dose LPS administration significantly impaired ovarian functions in mice, as evidenced by extended lengths of estrous cycles, reduced counts of primordial follicles, and alterations in the levels of serum hormones. Also, LPS administration promoted granulosa cell apoptosis and ovarian fibrosis in mice. However, LPS but not CTX significantly promoted IL-1β expression and production in mice. Moreover, LPS treatment but not CTX significantly enhanced TLR, p-p65, p65, and MyD88 protein expression in mouse ovaries, suggesting that LPS differs from CTX in triggering ovarian inflammation. In general, continuous low-dose LPS stimulation was less potent than high-dose LPS stimulation in the above-mentioned effects.Conclusions: LPS induces ovarian inflammation, fibrosis, and granulosa cell apoptosis and can be used to establish a POI model in mice.
Reduced 25-Hydroxyvitamin D Concentration in the Aqueous Humor of Cataract Patients with Open-Angle Glaucoma
Authors: Cho Yongwun , Yun Seung Pil , Yoo Woong-Sun , Kim Rock-Bum , Cho Min-Chul , Kim Seong-Jae 
Published: June 2021

Abstract
Previous epidemiological studies have demonstrated that the lower serum concentration of vitamin D was associated with elevated risk of open-angle glaucoma (OAG). However, few studies have examined the association between aqueous humor vitamin D concentrations and OAG. Hence, we investigated the relationship between 25-hydroxyvitamin D (25(OH)D) concentrations in aqueous humor and OAG. We measured 25(OH)D concentrations in aqueous humor and serum of 126 patients who underwent cataract surgery. The 25(OH)D concentrations were measured using Elecsys Vitamin D Total Kits with the Cobas e602 module (Roche Diagnostics, Mannheim, Germany), an electrochemiluminescence assay. Multiple linear regression analysis was performed to investigate factors associated with serum and aqueous humor 25(OH)D concentrations.Patients with OAG had significantly lower 25(OH)D concentrations in aqueous humor than control patients. Serum 25(OH)D concentrations were higher in patients with OAG than in the control, but this was not statistically significant. 25(OH)D concentrations in aqueous humor of patients with OAG were significantly associated with axial length but not with glaucoma severity, which was determined by the retinal nerve fiber layer thickness or mean deviation.Vitamin D concentrations in aqueous humor of patients with OAG were significantly lower than those in patients without OAG.
Gender Differences in Characteristics of Violent and Sexual Victimization in Patients With Psychosis: a Cross-sectional Study.
Authors: Stouwe E.C.D. van der , Steenhuis L.A. , Pijnenborg G.H.M. , de Vries B. , Bartels-Velthuis A.A. , Castelein S. , Veling W. , Visser E. , Busschbach J.T. 
Published: June 2021

Abstract
IntroductionVarious studies have demonstrated that individuals with a psychotic disorder are at an increased risk of becoming a victim of crime. Little is known about gender differences in victimization types and in specific characteristics of victimization (e.g., perpetrator, location or disclosure). Knowledge on characteristics of victimization would provide clinicians with more insight which may be especially useful for tailoring interventions. The aim of this study is to examine gender differences in characteristics of violent and sexual victimization in patients with a psychotic disorder.MethodsInformation on violent (threats, physical abuse) and sexual victimization (harassment, assault) was assessed in 482 individuals with a psychotic disorder who received mental health care. Patients were recruited through a routine outcome monitoring study and a clinical trial.ResultsMen reported more threats with violence (20.7% vs. 10.5%, x2=7.68, p=0.01), whereas women reported more sexual assault (13.3% vs. 3.6%, x2=15.43, p<0.001). For violent victimization, women were more likely than men to be victimized by a partner, friend or family member (52.9% vs. 30.6%) as opposed to a stranger (11.8% vs. 40.3%; O.R.=52.49) and to be victimized at home (60.0% vs. 29.3%) as opposed to on the street or elsewhere (40.0% vs. 70.3%; O.R.=0.06). For sexual victimization, there was no difference in location and perpetrator between men and women. For sexual victimization and physical violence, no differences in disclosure were found, but women were more likely not to disclose threats with violence or to disclose threats to a professional or police (52.9% vs. 45.2%; O.R.=30.33). All analyses were controlled for age, diagnosis and employment.DiscussionGender patterns of victimization types and characteristics are similar for individuals with a psychotic disorder in comparison to the general population. Men were at higher risk of violent victimization, whereas women were at higher risk for sexual victimization. Men were more likely to become victimized in the streets or elsewhere by a stranger, whereas women seemed to be more often victimized at home by a partner, friend or a family member. Future studies may tailor interventions preventing victimization in psychosis according to gender.
Traffic Flow Prediction with Relevance Vector Machine
Authors: Xiong W.-Z. , Shen X.-M. , Li H.-J. , Shen Z. 
Published: June 2021

Abstract
Real-time prediction of traffic flow values in a short period of time is an importantelement in building a traffic management system. The uncertainty, complexity andnonlinearity of traffic flow data make it difficult to predict traffic flow in real time,and the accurate traffic flow prediction has been an urgent problem in the industry.Based on the research of scholars, a traffic flow prediction model based on thecorrelation vector machine method is constructed. The prediction accuracy of thecorrelation vector machine is better than that of the logistic regression and supportvector machine methods, and the correlation vector machine method has the functionof generating prediction error range for the actual traffic sequence data. Theprediction results are very satisfactory, and the prediction speed is significantlyfaster than the other two models, which meets the requirement of real-time trafficflow prediction and is suitable for real-time online prediction, and the predictionaccuracy of the used method is relatively high. The three-way comparison analysisshows that the traffic flow prediction by the correlation vector machine methodcan describe the nonlinear characteristics of traffic flow change more accurately,and the model performance and real-time performance are better. The case studyshows that the traffic flow prediction model based on the correlation vector machinecan improve the speed and accuracy of prediction, which is very suitablefor traffic flow prediction estimation with real-time requirements, and provides ascientific method for real-time traffic flow measurement.
Association between Preoperative Toe Perfusion Index and Maternal Core Temperature Decrease during Cesarean Delivery Under Spinal Anesthesia: A Prospective Cohort Study
Authors: Kaneko Shohei , Hara Kentaro , Sato Shuntaro , Nakashima Takaya , Kawazoe Yurika , Taguchi Miyako , Urabe Shigehiko , Nakao Akiha , Hamada Kozue , Yamaguchi Michiko , Hara Tetsuya 
Published: June 2021

Abstract
Background: The main mechanism of temperature decrease during spinal anesthesia for cesarean delivery is core-to-peripheral redistribution of body heat, attributable to vasodilation. Perfusion index (PI) obtained with a pulse oximeter helps to assess peripheral perfusion dynamics by detecting changes in peripheral vascular tone. This study aimed to examine whether preoperative toe PI could predict spinal anesthesia-induced core temperature decrease during cesarean delivery.Methods: Parturients undergoing scheduled cesarean delivery under combined spinal-epidural anesthesia from September 2019 to March 2020 were enrolled in this single-center prospective cohort study. All parturients received 0.5% hyperbaric bupivacaine (10 mg) with fentanyl (15 µg) intrathecally. A pulse oximeter probe was placed on the left second toe for continuous PI measurement. The 3M™ Bair Hugger™ Temperature Monitoring System placed over the right temporal region was used to record core temperature over time. We evaluated the association between the maximum core temperature decrease, which is the primary outcome, and the preoperative toe PI at operating room (OR) admission using a segmented regression model (SRM) and a generalized additive model (GAM). The maximum core temperature decrease was defined as the difference between core temperature at OR admission and minimum intraoperative core temperature.Results: Forty-eight patients were evaluated. In the SRM, the slope for the association between the maximum core temperature decrease and the preoperative toe PI changed from 0.031 to 0.124 after PI = 2.4%. Likewise, with the GAM, there was a small core temperature decrease when preoperative toe PI was greater than 2.0% to 3.0%.Conclusions: A lower preoperative toe PI was associated with maternal core temperature decrease during cesarean delivery under spinal anesthesia. Preoperative toe PI is a simple, non-invasive, and effective tool for the early prediction of perioperative core temperature decrease during cesarean delivery.Trial registration: UMIN Clinical Trials Registry (registry number: UMIN000037965).URL: https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000042953
Impact of Induction Chemotherapy With Intermediate-Dosed Cytarabine and Subsequent Allogeneic Stem Cell Transplantation on the Outcome of High-Risk Acute Myeloid Leukemia
Authors: Fleischmann Maximilian , Schnetzke Ulf , Frietsch Jochen J , Sayer Herbert G , Schrenk Karin G , Hammersen Jakob , Glaser Anita , Hilgendorf Inken , Hochhaus Andreas , Scholl Sebastian 
Published: June 2021

Abstract
BackgroundAcute myeloid leukemia (AML) with antecedent hematological disease (s-AML) and treatment-related AML (t-AML) predict poor prognosis. Intensive treatment protocols of those highrisk patients should consider allogeneic stem cell transplantation (ASCT) in first complete remission (CR). Despite ASCT, relapse rate remains high. Induction chemotherapy with liposomal cytarabine and daunorubicin (CPX-351) has been approved for patients with AML with myeloid-related changes (AMLMRC) or t-AML based on improved survival and remission rates compared to standard 7+3 induction. Patients and Methods110 patients with newly diagnosed s-AML or t-AML at a university hospital were analyzed retrospectively. Median age was 62 years (24-77 years). A total of 65 patients with s-AML after MDS (59%) and 23 patients (20.9%) with t-AML were included. Induction chemotherapy consisted of intermediate-dosed cytarabine (ID-AraC) in combination with idarubicin (patients up to 60 years) or mitoxantrone (patients over 60 years). In patients subsequently undergoing ASCT reduced conditioning regimens (RIC) were applied prior to transplantation in 47 of 62 patients (76%). ResultsInduction chemotherapy with ID-AraC resulted in an overall response rate of 83% including complete remission (CR/CRi) in 69 patients (63%) with a low rate of early death (2.7%). Most relevant non-hematologic toxicity consisted of infectious complications including sepsis with need of intensive care treatment in five patients (4.5%) and proven or probable invasive fungal disease in 8 patients (7.2%). Relapse-free survival (RFS), event-free survival (EFS) and overall survival (OS) of the whole cohort were 19 months (0-167), 10 months (0-234) and 15 months (0-234), respectively (p<0.0001). A significant improvement of OS was observed in patients who underwent ASCT compared to those without subsequent ASCT: 9 months vs. 46 months, p<0.0001. Rate of transplantation-related mortality (TRM) in the early phase post ASCT was low (0.9% at day 30 and 1.8% at day 90, respectively). RIC conditioning results in OS rate of 60% after 60 months post ASCT (median OS not reached). ConclusionS-AML and t-AML patients receiving induction chemotherapy with intermediate-dosed cytarabine showed satisfactory response rate and consolidation therapy with ASCT after full or reduced intensity conditioning further improved survival in these patients with similar outcome as reported for CPX-351.
Wijsman Asymptotic Lacunary I_2-Invariant Equivalence for Double Set Sequences
Authors: Ulusu Uğur , Dündar Erdinç , Akın Nimet Pancaroğlu 
Published: June 2021

Abstract
In this study, for double set sequences, we present the notions of Wijsman asymptotic lacunary invariant equivalence, Wijsman asymptotic lacunary I_2-invariant equivalence and Wijsman asymptotic lacunary I_2^*-invariant equivalence. Also, we examine the relations between these notions and Wijsman asymptotic lacunary invariant statistical equivalence studied in this field before.
Are S-PRG Based Composites Able to Resist and Protect the Adjacent Enamel Against Erosive Wear?
Authors: Bergantin Bianca Tozi Portaluppe , Leone Camilla Cristina Di , Silva Thiago Cruvinel da , Wang Linda , Buzalaf Marília Afonso Rabelo , Borges Alessandra Buhler , Honório Heitor Marques , Rios Daniela 
Published: June 2021

Abstract
This in-vitro study evaluated the resistance of S-PRG-based-composites against erosive wear and their protective effect on enamel adjacent to restoration. Bovine-enamel-blocks were randomized into 12 groups (n=10/group), according to the factors material and type of wear (erosion-e or erosion+abrasion-a): S-PRG-based-composite-Beautifil II®(SPRGe/SPRGa); S-PRG-based bulk-fill-Beautifil Bulk Restorative®(SPRGBFe/SPRGBFa); composite-Filtek Z350 XT®(RCe/RCa); bulk-fill-composite-Filtek Bulk Fill®(BFe and BFa); glass-ionomer cement-EQUIA Forte®(GICe/GICa); resin-modified glass-ionomer cement-Riva®(RMGICe/RMGICa). Standardized cavities were prepared in specimens and restored. Initial profile was performed on the material and on the adjacent enamel at distances of 100/200/300/600 and 700μm. Specimens were immersed in 0.5%citric-acid (2min-6x/day-during 5days), and abrasive challenge was performed using a toothbrushing-machine (1min-after erosive challenge). Final profile was obtained following initial. Data were analyzed by two-way ANOVA and Tukey-test (α<0.05). On erosion, the GICe and RMGICe groups presented greater loss of material compared to other groups; up to 300μm away from the restoration, GICe and SPRGBFe were able to promote less enamel loss than composite groups. For erosion+abrasion S-PRG-based groups showed intermediate material wear compared to GICs (higher wear) and composites (less wear); there was no difference of enamel wear adjacent to restorations among groups. It is concluded that S-PRG-based-composites are a good alternative for restorative treatment of erosive tooth wear.Clinical Significance: S-PRG-based composite restorations are able to diminish surrounding enamel erosive wear, similarly to glass ionomer cement, with the advantage of being more resistant to erosive challenge. Therefore, this material is a potential option to restore advanced erosion lesions in patients with etiological factors still present.
Tribological and Machinability Performance of Hybrid Al2O3 -MWCNTs MQL for Milling Ti-6Al-4V
Authors: Jamil Muhammad , He Ning , Zhao Wei , Khan Aqib Mashood , Gupta Munish Kumar 
Published: June 2021

Abstract
Recent burgeoning development in nanotechnology unfold an avenue in the manufacturing industry. Owing to the superior heat transfer potential of nanoadditives mentioned recently, it could be interesting to improve the heat transfer and tribological capability of metal cutting fluids by mixing nanofluids in emulsions properly. In order to attain high-performance cutting of difficult-to-cut alloys, hybrid nanofluids assisted Minimum Quantity Lubrication (MQL) system is applied with the anticipation of efficient lubrication and heat transfer. Taguchi based L16(43) orthogonal array is used involving nanofluids concentrations of alumina-multiwalled carbon nanotubes (Al2O3-MWCNTs) air pressure and cooling flow rate at constant cutting conditions in the milling of Ti-6Al-4V. The resultant cutting force (FR), cutting temperature (T), and surface roughness (Ra) is considered as key machining responses. Besides, tool wear, chip analysis, and surface topography are also analyzed under the effect of hybrid nanofluids. Findings have shown the minimum resultant force, cutting temperature and surface roughness of 24.3N, 148.7oC, and 0.67µm respectively at nanofluids concentration of 0.24vol%, 120ml/h of flow rate at 0.6MPa of air pressure. The microscopic analysis of the end-mill depicted minor thermal damage, chip-welding, and coating peeling. Also, chip analysis depicts the clean back surface and less melting of saw-tooth chip edges. The surface topography confirms the less micro-adhesion of chips and material debris. The summary showed that appropriately chosen MQL parameters have improved the lubrication/cooling performance by providing oil film and enhancing the milling performance measures. The outcomes of the proposed study are useful for the manufacturing industry for the enhancement of process performance.
Identification of Hub Genes Associated with Development of Lung Adenocarcinoma by Integrated Bioinformatics Analysis
Authors: Li Jinglei , Hou Wei 
Published: June 2021

Abstract
Purpose: Lung adenocarcinoma (LUAD) has high heterogeneity and poor prognosis, posing a major challenge to human health worldwide. Therefore, it is necessary to improve our understanding of the molecular mechanism of LUAD in order to be able to better predict its prognosis and develop new therapeutic strategies for target genes.Methods: The Cancer Genome Atlas and Gene Expression Omnibus, were selected to comprehensively analyze and explore the differences between LUAD tumors and adjacent normal tissues. Critical gene information was obtained through weighted gene co-expression network analysis (WGCNA), differential gene expression analysis, and survival analysis.Results: Using WGCNA and differential gene expression analysis, 29 differentially expressed genes were screened. The functional annotation analysis showed these genes to be mainly concentrated in heart trabecula formation, regulation of inflammatory response, collagen-containing extracellular matrix, and metalloendopeptidase inhibitor activity. Also, in the protein–protein interaction network analysis, 10 central genes were identified using Cytoscape's CytoHubba plug-in. The expression of CDH5, TEK, TIMP3, EDNRB, EPAS1, MYL9, SPARCL1, KLF4, and TGFBR3 in LUAD tissue was found to be lower than that in the normal control group, while the expression of MMP1 in LUAD tissue was higher than that in the normal control group. According to survival analysis, the low expression of MYL9 and SPARCL1 was correlated with poor overall survival in patients with LUAD. Finally, through the verification of the Oncomine database, it was found that the expression levels of MYL9 and SPARCL1 were consistent with the mRNA levels in LUAD samples, and both were downregulated.Conclusion: Two survival-related genes, MYL9 and SPARCL1, were determined to be highly correlated with the development of LUAD. Both may play an essential role in the development LUAD and may be potential biomarkers for its diagnosis and treatment in the future.
Association Between Serum Microcystins Levels and Chronic Pelvic Inflammatory Disease: a Case-control Study
Authors: Zhu Na , Wang Xiaolan , Tan Jia , Xu Shuaishuai , Peng Yanhong , Gao Hong 
Published: June 2021

Abstract
Microcystins(MCs) have been reported to be closely related to the occurrence and development of inflammation by animal and cell experiments, but there are no study on the relationship between serum microcystin-LR(MC-LR) and chronic pelvic inflammatory disease (CPID) risk in populations. We designed a clinical case-control study to investigate the relationship between serum MC-LR and CPID risk. From October 2020 to March 2021, 50 patients diagnosed with CPID and 50 controls (frequency matched by age) were recruited from the First Hospital University of South China, in Hengyang, Central China. The basic information on lifestyle and history of disease was acquired through questionnaires. Blood samples were analyzed for MC-LR by ELISA. Binary logistic regression analyses and chi-square test were used to evaluate the effects of MC-LR on CPID risk. With the increase of serum MC-LR level (Q2, Q3 and Q4), the AOR of CPID risk increased (0.139, 0.167 and 0.040, respectively). The serum MC-LR(0.06 ~ 0.66µg/L) was an independent protective factor for CPID in humans, and the protective effect of concentrations ≥ 0.25μg/L was more obvious. Within the certain concentration range, MC-LR was an independent protective factor for the risk of CPID in humans, which will provide a scientific basis for the study of the relationship between serum microcystins and inflammation.
Adhesive Ability of Different Oral Pathogens to Various Dental Materials: An in Vitro Study
Authors: Tu Yan , Lin Xiaolong , Wang Yuan , Deng Shuli 
Published: June 2021

Abstract
This study provides a basis for selecting dental materials and lays a foundation for developing new dental materials. Four dental restorative materials were divided into two groups: Streptococcus mutans and Actinomyces viscosus, five root canal sealing materials were divided into two groups: Porphyromonas gingivalis and Enterococcus faecalis. Each material block was immersed in the corresponding group of the bacterial solution and cultured under anaerobic conditions at 37°C for 2, 4, 6, 8, 12, 16, 20, and 24 h. The adhesion of bacteria was observed, the number of different bacteria adhering to various material model disks was calculated at different time intervals under a scanning electron microscope. 24 hours later, the number of Streptococcus mutans and Actinomyces viscosus that adhered to the surface of the resin and the zinc phosphate cement material disks was the maximum. The number of Porphyromonas gingivalis and Enterococcus faecalis adhering to the surface of the AH Plus sealer block was the maximum. Streptococcus mutans and Actinomyces viscosus exhibited the strongest adhesion ability to the resin and the zinc phosphate cement material block. Porphyromonas gingivalis and Enterococcus faecalis showed the highest adhesion ability to the AH Plus sealer block.