bluuebunny/crossref_metadata_2025_split huggingface dataset:
AgNPs Improved Feather Keratin Based Bio-Membrane
Authors: Ding Jiao , Lai Ruihao , Chen Wenjie , Zhu Guodian , He Ming , Huang Suqing , Yin Guoqiang 
Published: October 2021

Abstract
AgNPs (Silver Nanoparticles) is successfully prepared and then loaded into feather keratin (FK) based bio-membrane by electrospinning. The morphology, thermal stability, mechanical properties and bacteriostatic activity of the as-prepared AgNPs/FK/PVA bio-membrane are completely developed in this study. Microstructure show that the AgNPs has been dispersed in FK-based bio-membrane without agglomeration. The results suggest that the addition of AgNPs enhanced effectively the performances of FK-based bio-membrane, and the appropriate amount of AgNPs is 1%-2%. The combination of AgNPs and FK can not only ensure the uniform dispersion and antibacterial stability of AgNPs, but also give play to the biocompatibility effect of FK, which makes it natural, safe, stable and degradable, and broaden its application in the field of biomedicine.
Medial Prefrontal and Occipito-Temporal Activity At Encoding Determine Enhanced Recognition of Threatening Faces After 1.5 Years
Authors: LIU Xiqin , Zhou Xinqi , Zeng Yixu , Li Jialin , Zhao Weihua , Xu Lei , Zheng Xiaoxiao , Fu Meina , Yao Shuxia , Cannistraci Carlo V. , Kendrick Keith M. , Becker Benjamin 
Published: October 2021

Abstract
Studies demonstrated that faces with threatening emotional expressions are better remembered than non-threatening faces. However, whether this memory advantage persists over years and which neural systems underlie such an effect remains unknown. Here, we employed an individual difference approach to examine whether the neural activity during incidental encoding was associated with differential recognition of faces with emotional expressions (angry, fearful, happy, sad and neutral) after a retention interval of > 1.5 years (N = 89). Behaviorally, we found a better recognition for threatening (angry, fearful) versus non-threatening (happy and neutral) faces after a > 1.5 years delay, which was driven by forgetting of non-threatening faces compared with immediate recognition after encoding. Multivariate principal component analysis (PCA) on the behavioral responses further confirmed the discriminative recognition performance between threatening and non-threatening faces. A voxel-wise whole-brain analysis on the concomitantly acquired functional magnetic imaging (fMRI) data during incidental encoding revealed that neural activity in bilateral inferior occipital gyrus (IOG) and ventromedial prefrontal/orbitofrontal cortex (vmPFC/OFC) was associated with the individual differences in the discriminative emotional face recognition performance measured by an innovative behavioral pattern similarity analysis (BPSA) based on inter-subject correlation (ISC). The left fusiform face area (FFA) was additionally determined using a regionally focused analysis. Overall, the present study provides evidence that threatening facial expressions lead to persistent face recognition over periods of > 1.5 years and differential encoding-related activity in the medial prefrontal cortex and occipito-temporal cortex may underlie this effect.
Role of Exogenous-Applied Salicylic Acid, Zinc And Glycine Betaine To Improve Drought-Tolerance In Wheat During Reproductive Growth Stages
Authors: Shemi Ramadan , Wang Rui , Gheith El-Sayed M.S. , Hussain Hafiz Athar , Cholidah Linna , Zhang Kangping , Zhang Sai , Wang Longchang 
Published: October 2021

Abstract
Background: Drought has become a dangerous threat to reduce crop productivity throughout the world. Exogenous application of regulators, micronutrients, or osmoprotectants for inducing drought-tolerance in field crops has been effectively adopted. A controlled pot study was performed to investigate the relative efficacy of salicylic acid (SA), zinc (Zn), and glycine betaine (GB) as foliar applications on the growth, tissues pigments content, relative water content (RWC), leaf gas exchange, antioxidant enzymes activity, reactive oxygen species (ROS) accumulation, osmolytes contents and the yield parameters of wheat plants subjected to two soil water conditions (85% field capacity: well-watered, 50% field capacity: water-deficient) during reproductive growth stages.Results: Water deficient conditions significantly decreased the growth, yield parameters, RWC, photosynthesis pigment, and gas exchange attributes except for intercellular CO2 concentration. However, foliar applications remarkably improved the growth parameters under water deficit conditions. Drought stress statistically increased the contents of hydrogen peroxide (H2O2), superoxide anion radical (O2 •−), and malonaldehyde (MDA), and elevated the harmful oxidation to cell lipids in plants, however, they were considerably reduced by foliar applications. Activities of all antioxidant enzymes, proline content, and soluble sugar were increased in response to foliar applications under water deficit conditions.Conclusions: Overall, foliar application of GB, SA, and Zn compounds improved the drought-tolerance in wheat by decreasing the ROS accumulation, promoting enzymatic antioxidants, and increasing osmolytes accumulation. Finally, GB treatment was most effective in thoroughly assessed parameters of wheat followed by SA and Zn applications to alleviate the adverse effects of drought stress.
Complications After Lymph Node Dissection Along Bilateral Recurrent Laryngeal Nerves Through Right Thoracic Approach in the Patients With Thoracic Esophageal Cancer
Authors: Yuan Ligong , Li Feng , Mao Yousheng , He Jie , Gao Shugeng , Xue Qi 
Published: October 2021

Abstract
Background: Extensive lymph nodes dissection can improve the accuracy of tumor staging and prognosis of the patients with thoracic esophageal cancer, palsy of recurrent laryngeal nerve (RLN) caused by the lymph node (LN) dissection along RLN chain also increase postoperative complications and may affect the prognosis. This study aimed to evaluate the associated postoperative complications after LN dissection along RLNs in the patients with thoracic esophageal squamous cell cancer (ESCC).Methods: 339 eligible patients with thoracic ESCC who underwent radical McKeown or Ivor-Lewis esophagectomy by open or VATS procedures through right thoracic approach with LN dissection along bilateral RLNs were included in this study. Univariate and multivariate logistic regression analysis were conducted to assess the correlation of RLN paralysis (RLNP) with other post-operative complications. Results: 39 of the 339 patients were diagnosed with RLNP (11.5%) postoperatively. The incidence of RLNP in three-field (3FL) LN dissection was significantly higher than that in the two-field (2FL) LN dissection ( 24.0% vs 8.0%, P＜0.001). Compared with the patients without RLNP, the patients with it had a significantly higher incidence of postoperative anastomotic leakage (P=0.029), pulmonary complications (P=0.001) and much longer hospital stay (P=0.001). Two patients died of respiratory failure within 30 days caused by RLNP and were treated by reintubation. Conclusion: RLNP after LN dissection along bilateral RLN in thoracic ESCC was associated with much higher morbidity such as pulmonary complications, anastomotic leakage, and much longer hospital stay. New technologies are required to reduce RLNP incidence and its associated complications.
Compact artificial neuron based on anti-ferroelectric transistor
Authors: Cao Rongrong , Zhang Xumeng , Liu Sen , Lu Jikai , Wang Yongzhou , Yang Yang , Sun Yize , Wei Wei , Wang Jianlu , Xu Hui , Li Qingjiang , Liu Qi 
Published: October 2021

Abstract
Neuromorphic machines based on spiking neural networks (SNNs) provide a more fascinating platform over traditional computers on building energy-efficient intelligent systems, in which spiking neuron are pivotal components. Recently, memristive neurons, with promising bio-plausibility and density, have been developed, but with limited reliability or bulky capacitors for integration or additional circuits for reset. Here, we propose a novel anti-ferroelectric field-effect transistor (AFeFET) neuron based on the inherent polarization and depolarization of Hf0.2Zr0.8O2 anti-ferroelectric film to meet these challenges. In this neuron, the intrinsic polarization accumulation effect in the Hf0.2Zr0.8O2 film increases the channel current of AFeFET gradually, which implements the integration feature of the neuronal membrane and avoids using external capacitors. Also, the spontaneous depolarization effect in AFeFET emulates the leaky behavior of neurons, saving the hardware overhead of neuron circuits by getting rid of external reset circuits. Moreover, the AFeFET neuron exhibits other comprehensive merits, such as low energy consumption (37 fJ/spike), excellent endurance (>1012), high uniformity and high stability. Using such an AFeFET neuron, we further construct a two-layer fully ferroelectric (784×400×10) SNN combining established FeFET synapse, achieving 96.8% recognition accuracy on MNIST datasets. This work opens the way to emulate spiking neurons with anti-ferroelectric materials and provides a more competitive approach to build high efficient neuromorphic hardware systems.
Over-expression of YTHDC2 Contributes to Progression of Prostate Cancer and Predicts Poor Outcome in Patients with Prostate Cancer
Authors: jukun song , You Ganhua , Yin Xinhai , Chen Weiming , Zhu Guohua , Wang Wei , Yu Yongwei , Zhu Jianguo 
Published: October 2021

Abstract
Objective: YTHDC2, a member of N6-methyladenosine (m6A) readers, has been reported to be closely associated with multiple cancer types. However, very little is known about YTHDC2 gene and its involvement in prostate cancer. Methods: YTHDC2 protein expression level was analyzed and correlated to clinical outcomes in prostate cancer patients who underwent prostatectomy in Guizhou Provinicial People’s Hospital. YTHDC2 expression level was also detected in prostate cancer cell lines and an immortalized prostate epithelial cell line BPH-1 and RWPE1 by qRT-PCR. Furthermore, we established stable cell lines (DU145 and PC-3) transfected with either empty vector or the full-length YTHDC2 gene and conducted cell function assays in vitro. Fisher’s exact test and Pearson χ2 test was employed, Kaplan-Meier method was used for the survival analysis.Result: Of 32 patient samples who enrolled in this study, YTHDC2 was significantly upregulated in PCa patients with higher Gleason score and Serum PSA level. YTHDC2 expression was significantly elevated in all PCa cell lines compared to BPH-1 and RWPE1 (all P<0.05). Functionally, the enforced expression of YTHDC2 markedly promoted cell growth, migration and invasion efficacies in prostate cancer cells.Conclusion: Our data indicate that YTHDC2 upregulation may be potentially associated with prognosis of prostate cancer patients. Trial registration: Retrospectively registered.
Fullerene Derivatives (CN-[OH]β) and Carbon Nanotubes Modelled as Transporters for Doxorubicin Drug in Cancer Therapy
Authors: Garalleh Hakim AL 
Published: October 2021

Abstract
Carbon nanomaterials have received increasing attention in drug delivery applications because of their distinct properties and structures, including large surface areas, high conductivity, low solubility in aqueous media, unique chemical functionalities and stability at the nano-scale size. Particularly, they have been used as nano-carriers and mediators for anticancer drugs such as, combination with Cisplatin, Camptothecin and Doxorubicin. Cancer has become the most challenging disease because its sophisticated therapy and classified as one of the top killers according to the World health organization records. The aim of the current work is to study and investigate the mechanism of combination between single-walled carbon nanotubes (SWCNTs) and the fullerene derivatives (C N -[OH] β ) as mediators, and anticancer drugs for photodynamic therapy directly to destroy the infected cells without damaging the normal ones. Here, we obtain a bio-medical model to determine the efficiency of usefulness of Doxorubicin (DOX) as an antitumor agent conjugated with SWCNTs with variant radii r and fullerene derivative (C N -[OH] β ). The two sub-models are obtained mathematically to evaluate the potential energy arising from the DOX-SWCNT and DOX-(C N -[OH] β ) interactions. DOX modelled as two-connected spheres, small and large, each interacting with different SWCNTs (variant radii r ) and fullerene derivatives C N -[OH] β , forming based on the number of carbon atoms (N) and the number of Hydroxide molecules (OH) ( β ), respectively.
Bioinformatic Analysis of lncRNA Mediated Competitive Endogenous RNA Network in Intestinal Ischemia/reperfusion Injury
Authors: Zhu Lin , Yang Xiao , Yao Zhiwen , Wang Ziyi , Lai Yupei , Xu Shiting , Liu Kexuan , Zhao Bingcheng 
Published: October 2021

Abstract
BackgroundRecently, an increasing number of studies have reported the roles of competitive endogenous RNA (ceRNA) networks in ischemia/reperfusion (I/R) injury, which include the liver, kidney, heart, brain, and intestine. However, the functions and mechanisms of long non-coding RNAs (lncRNAs), which serve as ceRNA networks in intestinal I/R injury, are still unclear. MethodsIn this study, bioinformatics methods were used to filter and construct the lncRNA-miRNA-mRNA networks in intestinal I/R injury. RNA expression data were retrieved from NCBI GEO datasets, the expression profiles between mouse small intestine with superior mesenteric artery occlusion and Sham operation was analyzed, and 189 microRNA differential expressed genes(miDEGs) were discovered successfully from miRNA GEO dataset (GSE83701). Next, targeted lncRNAs and mRNA in the database were matched based on miDEGs. Then, lncRNA-miRNA-mRNA networks were constructed with Cytoscape. The hub lncRNA-miRNA-mRNA networks were selected via Cytoscape plug-in CytoHubba and intersected mRNAs of datasets GSE96733 and GSE83701. Finally, the vital nodes of the ceRNA networks were validated by qPCR. ResultsThe 1700020114Rik/mmu-miR-7a-5p/Klf4 axis was postulated to play a potential role in intestinal I/R injury.ConclusionThe results shed novel insight into the molecular mechanism of ceRNA networks in intestinal I/R injury and highlighted the potential of 170002700020114Rik/mmu-miR-7a-5p/Klf4 ceRNA network in the prevention and treatment of intestinal I/R injury.
Synergetic Effect of Lipoprotein(a) and Lipoprotein-Associated Phospholipase A2 on Functional Outcomes in Patients with Ischemic Stroke
Authors: Zheng Lemin , Jiang Xue , Xu Jie , Hao Xiwa , Xue Jing , Li Ke , Jin Aoming , Lin Jinxi , Meng Xia , Wang Yongjun 
Published: October 2021

Abstract
Background:The relationship of lipoprotein(a) [Lp(a)] and stroke functional outcomes was conflicting. The relationship of Lp(a) and Lp-PLA2 levels to functional outcomes is unclear. The aim was to clarify whether high Lp(a) is associated with poor functional outcomes and examine the relationship of Lp(a) and Lp-PLA2 to functional outcomes in patients with ischemic stroke.Methods：A total of 10,422 individuals from the third China National Stroke Registry cohort were recruited. Plasma level of Lp(a) at admission was measured with enzyme-linked immunosorbent assay. The cut-off was set at the median for Lp(a). Functional outcome was assessed using the modified Rankin scale (mRS) at 3 months after stroke. The association between Lp(a) and stroke functional outcomes was evaluated using a multivariate Cox regression model.Results：The median age was 63.0 years, and 31.6% participants were women. Patients in higher Lp(a) group had higher incidences of poor functional outcome at 3 months (P<0.0001). In multivariate cox regression model, elevated Lp(a) levels were associated with poor functional outcomes at 3 months (Q4 vs. Q1: hazard ratio 1.39, 95% confidence interval 1.11-1.75). Subgroup analysis showed the significant effect of interaction of Lp-PLA2 level with Lp(a) level on functional outcomes (p=0.008). After stratification by Lp(a) and Lp-PLA2, the Lp(a) high/ Lp-PLA2 high group showed the highest incidence of poor functional outcomes at 3 months.Conclusions:Elevated Lp(a) level is associated with poor functional outcomes in patients with ischemic stroke. Lp(a) has a synergetic effect with Lp-PLA2 on functional outcomes after ischemic stroke.
Comparison of Left Lower Entrance and Traditional Laparoscopic Greater Omentum Resection for Stage I Ovarian Cancer
Authors: Le Aiwen , Yang Fan , Kang Kai , He Guna 
Published: October 2021

Abstract
Objective : To describe the surgical method, safety and convenience of " lower left entrance laparoscopic greater omentum resection" and evaluate its clinical application value for stage I ovarian cancer. Methods: 31 patients with early stage I ovarian cancer underwent with laparoscopic staging surgery adopted " lower left entrance laparoscopic omentum resection " as the observation group, and 29 cases underwent by conventional laparoscopy as control group. The intraoperative and postoperative indexes were compared. Results: During the greater omentum resection, there was no significant difference between two groups in the blood loss, but the operation time in observation group was significantly shorter than the control group. There was no difference in postoperative hospital stay、gastrointestinal exhaust time and postoperative severe complications.Conclusion: " lower left entrance laparoscopic greater omentum resection " may be a safe and effective technique in stage I ovarian cancer.
Promotion of in Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling
Authors: Chen Fengjiao , Yang Ying , Chen Jianling , Tang Zihua , Peng Qian , Wang Jinfu , Ding Jie 
Published: October 2021

Abstract
Background Notch signaling mediates the committed induced differentiation of ear sensory cells and promotes the formation of a precise arrangement of mosaics between hair cells and supporting cells. Embryonic stem cells (ESCs) are pluripotent stem cells which have the potential to differentiate into cell lines through three germ layers. Therefore, it is necessary to study the effects of regulating Notch receptors and ligand expression on the in vitro differentiation equilibrium of hair cells and supporting cells from ESCs.Methods and Results The temporal ex-pression pattern of Notch ligands and receptors during in vitro hair cell-like cell differentia-tion from human embryonic stem cells (hESCs) was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Subsequently, pAJ-U6-shRNA-CMV-Puro/GFP recombinant lentiviral vectors, encoding short hairpin RNAs, were used to silence JAG-1, JAG-2, and DLL-1, according to the temporal expression pattern of Notch ligands. Then the effect of each ligand on the in vitro differentiation of hair cells was examined by RT-PCR, immunofluorescence, and scanning electron microscopy (SEM).Conclusions Results showed that JAG-1 played an important role in regulating hESC differentiation to otic progenitors. The individual deletion of JAG-2 or DLL-1 had no significant effect on the differentiation of hair cell-like cells. Although the simultaneous inhibition of both DLL-1 and JAG-2 could increase the number of hair cell-like cells, it decreased the number of supporting cells.
Null Results for the Steal-Framing Effect on Out-Group Aggression
Authors: Mifune Nobuhiro 
Published: October 2021

Abstract
Whether intergroup conflict is a necessary condition for the evolution of human prosociality has been a matter of debate. At the center of the debate is the coevolutionary model of parochial altruism—that human cooperation with in-group members has coevolved with aggression toward out-group members. Studies using the intergroup prisoner’s dilemma–maximizing difference game to test the model have repeatedly shown that people do not exhibit out-group aggression, possibly because of an inappropriate operationalization and framing of out-group aggression. The coevolutionary model predicts out-group aggression when the actor understands that it will lead to the in-group’s benefit. However, in the game, such an aspect of out-group aggression that benefits the in-group is typically not well communicated to participants. Thus, this study tested the hypothesis that out-group aggression in the game would be promoted by a framing that emphasizes that attacking out-group members enhances the in-group’s gain. Results of two laboratory experiments with 176 Japanese university students in total showed that such a framing did not promote out-group aggression and individuals invested more money to cooperate with in-group members only, avoiding the strategy of cooperating with in-group members to harm out-group members. These results do not support the coevolutionary model.
Metavirome Survey of Eight Ray-Finned Fishes Revealed Domination of Giant Viral Members of Pandoravirus and Megaviricetes
Authors: Askari Fatemeh Sana , Mohebbi Alireza 
Published: October 2021

Abstract
Background

Fishes are an essential source of food all around the world. The bulk of the microbial diversity in marine ecosystems is uncharacterized, with viruses accounting for most of the remaining diversity. This may be a major danger to public health, therefore knowing the variety of viruses in the marine ecosystem is critical. The goal was to assess the virome of ray-finned fishes in terms of variety and relation to human diseases.
Results

It was revealed that the viral kingdom was allocated 2,228,888 sequence reads. The pooled genome assay of the ray-finned fishes indicated a significant abundance of viruses in three clades of the genus Pandoravirus (31%), the order of Herepesvirales (23%), and the kingdom of Bamfordvirae (10%). Furthermore, 432281/2031445 reads (21%) of mapped reads were unassigned viruses. According to the GiantVirusFinder findings, 17931 hits (4.15$) were mapped to the genomes of known giantviruses.
Conclusion

Humans eat seafood regularly as one of their primary diets. Ray-finned fishes have a long evolutionary history and, as demonstrated here, are hosts to a variety of large DNA viruses. Fish must be screened for viral infections that are linked to human illnesses.
Bladder Cancer Biomarker Screening based on Non-targeted Urine Metabolomics
Authors: Li Jinkun , Cheng Bisheng , Zhan Chuanchuan , Li ShiPeng , Bai Peiming 
Published: October 2021

Abstract
Background: Bladder cancer is one of the most common malignancies of the urinary system, and its screening relies heavily on invasive cystoscopy, which increases the risk of urethral injury and infection. This study aims to use non-targeted metabolomics approaches to screen the metabolites that are expressed significantly differently from the urine of bladder cancer patients and the cancer-free controls. Methods and Results: This study performed non-targeted metabolomic analysis and metabolite identification on the urine of bladder cancer patients (n=57) and the cancer-free controls (n=38) using a liquid chromatography-mass spectrometry analyzer. The results showed that there were significant differences in the expression of 27 metabolites between bladder cancer patients and the cancer-free controls. Conclusions: In the multivariate statistical analysis of this study, the urinary metabolic profile data of bladder cancer patients are analyzed, and the receiver operating characteristic curve analysis shows that it is possible to perform non-invasive clinical diagnoses of bladder cancer through these candidate biomarkers.