bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stronger together: Analytical techniques for recombinant adeno associated virus
Authors: Hutanu Andrei , Boelsterli David , Schmidli Claudio , Montealegre Cristina , Dang Thai Mike H. N. , Bobaly Balazs , Koch Marius , Schwarz Maria A. 
Published: November 2021

AbstractWith recent FDA approval of two recombinant adeno‐associated virus (rAAV)‐based gene therapies, these vectors have proven that they are suitable to address monogenic diseases. However, rAAVs are relatively new modalities, and their production and therapy costs significantly exceed those of conventional biologics. Thus, significant efforts are made to improve the processes, methods, and techniques used in manufacturing and quality control (QC). Here, we evaluate transmission electron microscopy (TEM), analytical ultracentrifugation (AUC), and two modes of capillary electrophoresis (CE) for their ability to analyze the DNA encapsidated by rAAVs. While TEM and AUC are well‐established methods for rAAV, capillary gel electrophoresis (CGE) has been just recently proposed for viral genome sizing. The data presented reflect that samples are very complex, with various DNA species incorporated in the virus, including small fragments as well as DNA that is larger than the targeted transgene. CGE provides a good insight in the filling of rAAVs, but the workflow is tedious and the method is not applicable for the determination of DNA titer, since a procedure for the absolute quantification (e.g., calibration) is not yet established. For estimating the genome titer, we propose a simplified capillary zone electrophoresis approach with minimal sample preparation and short separation times (<5 min/run). Our data show the benefits of using the four techniques combined, since each of them alone is prone to delivering ambiguous results. For this reason, a clear view of the rAAV interior can only be provided by using several analytical methods simultaneously.
Where does purging disorder lie on the symptomatologic and personality continuum when compared to other eating disorder subtypes? Implications for the DSM
Authors: Krug Isabel , Giles Sarah Elizabeth , Granero Roser , Agüera Zaida , Sánchez Isabel , Sánchez‐Gonzalez Jessica , Jimenez‐Murcia Susana , Fernandez‐Aranda Fernando 
Published: November 2021

AbstractObjectivesTo assess the clinical significance and distinctiveness of purging disorder (PD) from other eating disorder (ED) diagnoses.MethodParticipants included 3127 women consecutively admitted to an ED treatment centre (246 PD, 465 anorexia nervosa restrictive [AN‐R], 327 AN‐binge purging [AN‐BP], 1436 bulimia nervosa [BN], 360 binge eating disorder [BED], 177 atypical AN and 116 unspecified feeding or eating disorder [UFED]) who were diagnosed according to DSM‐5 criteria. Additionally, 822 control participants were recruited from the community. All participants completed measures assessing ED symptoms (EDI‐2), general psychopathology (SCL‐90‐R) and personality (TCI‐R).ResultsPatients with PD, when compared to controls, scored significantly higher on the EDI‐2 and SCL‐90‐R, and most TCI‐R dimensions. Most of the significant differences between PD and the other ED diagnoses emerged between PD and AN‐R, followed by Atypical‐AN, UFED, AN‐BP and BED, with patients with PD typically reporting higher scores on the EDI‐2 and SCL‐90‐R subscales. Significant differences between PD and BN were also present, but to a lesser extent. The findings for personality varied amongst the different ED diagnoses.ConclusionsPD is a clinically significant disorder, which seems to be more similar to BN than it is to AN and the other ED subtypes.
ANCHORS: A multi‐decadal tide gauge dataset to monitor Australian relative sea level changes
Authors: Hague Ben S. , Jones David A. , Trewin Blair , Jakob Doerte , Murphy Bradley F. , Martin David J. , Braganza Karl 
Published: November 2021

AbstractHere, we describe a new national tide gauge‐based sea level dataset for monitoring sea level changes around Australia, and the novel homogenization methodology used to develop it. Homogenization is a two‐step process that involves the detection of jumps or steps in the data (inhomogeneities) followed by a correction applied to remove the change. This new dataset is called the Australian National Collection of Homogenized Observations of Relative Sea Level (ANCHORS). ANCHORS provides coastal sea levels at hourly resolution with homogenization performed on annual means. ANCHORS is intended to be a national tide gauge‐based sea level dataset to monitor changes in mean sea level and resultant coastal flood frequency changes around Australia. Whilst sea levels are rising around Australia in response to global mean sea level changes, coastal water levels represent a notable gap in the suite of in situ high‐quality datasets available for researchers, governments and industry. Tide gauge datasets are well suited to impact‐based assessments of changes in flood frequency, duration and risk. In this regard, ANCHORS will sit alongside existing satellite‐ and numerical modelling‐based sea level datasets to provide enhanced insights into the physical impacts of sea level rise on Australian coastlines. We have adapted established homogenization techniques to develop a national collection of homogenous long tide gauge records. The dataset comprises 38 tide gauge records from around Australia, varying in length from 34 to 123 years, with an average of 57 years. Finally, we discuss limitations and potential applications for ANCHORS and how these have informed the design of this coastal sea level dataset.
How do community based dementia friendly initiatives work for people with dementia and their caregivers, and why? A rapid realist review
Authors: Thijssen Marjolein , Daniels Ramon , Lexis Monique , Jansens Rianne , Peeters José , Chadborn Neil , Nijhuis‐van der Sanden Maria W. G. , Kuijer‐Siebelink Wietske , Graff Maud 
Published: November 2021

AbstractObjectivesTo describe and explain the outcomes of community dementia friendly initiatives (DFIs) for people with dementia and their caregivers to inform the development and tailoring of DFIs.MethodsLiterature searches on DFIs were performed through two systematic online database searches of PubMed, Embase, ASSIA, CINAHL and Google scholar. Papers were only included if they evaluated outcomes using empirical data from people with dementia or caregivers. Data collection and analysis were guided by the categorization in the DEM‐FACT taxonomy and RAMESES guidelines for realist reviews.ResultsOf 7154 records identified, 22 papers were included with qualitative, mixed method and quantitative study designs. The synthesis led to a description of programme theories addressing caring, stimulating and activating communities. Outcomes for people with dementia and caregivers included having contact with others, enjoyment and decrease of stress and, lastly, support. This synthesis also indicated how people with dementia participated in a specific role in DFIs, such as patient, team member or active citizen.ConclusionsDFIs generate different outcomes for people with dementia and caregivers, depending on the kind of initiative and the specific role for people with dementia. These findings could be a catalyst for initiation and further development of DFIs in a dementia friendly community (DFC). This draws attention to the multiple aspects of DFCs and supports reflection on their essential principles.
PLS‐SEM: Prediction‐oriented solutions for HRD researchers
Authors: Legate Amanda E. , Hair Joe F. , Chretien Janice Lambert , Risher Jeffrey J. 
Published: November 2021

AbstractStructural equation modeling, often referred to as SEM, is a well‐established, covariance‐based multivariate method used in Human Resource Development (HRD) quantitative research. In some research contexts, however, the rigorous assumptions associated with covariance‐based SEM (CB‐SEM) limit applications of the method. An emergent complementary SEM approach, partial least squares structural equation modeling (PLS‐SEM), is a variance‐based SEM method that provides valid solutions and overcomes several limitations associated with CB‐SEM. Despite PLS‐SEM's increasing popularity in many social sciences disciplines, the method has yet to gain traction in the field of HRD. An accessible overview of the method, including potential advantages for HRD research and extant methodological advancements, is provided in this article with the goal of encouraging productive dialogue in the field of HRD surrounding the PLS‐SEM approach. We present an emergent analytical tool for quantitative HRD research, offer practical guidelines for researchers to consider when selecting a SEM method, and clarify assessment stages and up‐to‐date evaluation criteria through an illustrative example.
Vulnerability of water resources under a changing climate and human activity in the lower Great Lakes region
Authors: Soonthornrangsan Jenny T. , Lowry Christopher S. 
Published: November 2021

AbstractGlobally, the number of people experiencing water stress is expected to increase by millions by the end of the century. The Great Lakes region, representing 20% of the world's surface freshwater, is not immune to stresses on water supply due to uncertainties on the impacts of climate and land use change. It is imperative for researchers and policy makers to assess the changing state of water resources, even if the region is water rich. This research developed the integrated surface water‐groundwater GSFLOW model and investigated the effects of climate change and anthropogenic activities on water resources in the lower Great Lakes region of Western New York. To capture a range of scenarios, two climate emission pathways and three land development projections were used, specifically RCP 4.5, RCP 8.5, increased urbanization by 50%, decreased urbanization by 50%, and current land cover, respectively. Model outputs of surface water and groundwater discharge into the Great Lakes and groundwater storage for mid‐ and late century were compared to historical to determine the direction and amplitude of changes. Both surface water and groundwater systems show no statistically significant changes under RCP 4.5 but substantial and worrisome losses with RCP 8.5 by mid‐century and end of century. Under RCP 8.5, streamflow decreased by 22% for mid‐century and 42% for late century. Adjusting impervious surfaces revealed complex land use effects, resulting in spatially varying groundwater head fluctuations. For instance, increasing impervious surfaces lowered groundwater levels from 0.5 to 3.8 m under Buffalo, the largest city in the model domain, due to reduced recharge in surrounding suburban areas. Ultimately, results of this study highlight the necessity of integrated modelling in assessing temporal changes to water resources. This research has implications for other water‐rich areas, which may not be immune to effects of climate change and human activities.
It matters how you start: Early numeracy mastery predicts high school math course‐taking and college attendance
Authors: Davis‐Kean Pamela E. , Domina Thurston , Kuhfeld Megan , Ellis Alexa , Gershoff Elizabeth T. 
Published: November 2021

AbstractUsing data from the Applied Problems subtest of the Woodcock‐Johnson Tests of Achievement (Woodcock & Johnson, 1989/1990, Woodcock‐Johnson psycho‐educational battery‐revised. Allen, TX: DLM Teaching Resources) administered to 1,364 children from the National Institute of Child Health and Human Development (NICHD) Study of Early Childcare and Youth Development (SECCYD), this study measures children's mastery of three numeric competencies (counting, concrete representational arithmetic and abstract arithmetic operations) at 54 months of age. We find that, even after controlling for key demographic characteristics, the numeric competency that children master prior to school entry relates to important educational transitions in secondary and post‐secondary education. Those children who showed low numeric competency prior to school entry enrolled in lower math track classes in high school and were less likely to enrol in college. Important numeracy competency differences at age 54 months related to socioeconomic inequalities were also found. These findings suggest that important indicators of long‐term schooling success (i.e., advanced math courses, college enrollment) are evident prior to schooling based on the levels of numeracy mastery.
Default predicted no-effect target concentrations for antibiotics in the absence of data for the protection against antibiotic resistance and environmental toxicity
Authors: Vestel Jessica , Caldwell Daniel J. , Tell Joan , Constantine Lisa , Häner Andreas , Hellstern Jutta , Journel Romain , Ryan Jim J. , Swenson Tim , Xei Wei 
Published: November 2021

Abstract
The pharmaceutical manufacturing industry, via the AMR Industry Alliance, has developed and implemented steps to help minimize the potential impact of pharmaceutical manufacturing on the spread of antimicrobial resistance (AMR). One of these steps was to publish predicted no-effect concentrations (PNECs) to serve as targets for antibiotic manufacturing wastewater effluent risk assessments aimed to help protect environmental receptors and to mitigate against the spread of antibiotic resistance. Concentrations below which adverse effects in the environment are not expected to occur (PNECs) were first published in 2018 and are updated annually. The current list now stands at 125 antibiotics; however, it is recognized that this list does not encompass all manufactured antibiotics. Therefore, a statistical evaluation of currently available data was conducted and a default PNEC of 0.05 µg/L for antibiotics in the absence of other data was derived. Integr Environ Assess Manag 2022;18:863–867. © 2022 Merck, Sanofi, Johnson & Johnson Services, Inc, F.Hoffmann-La Roche Ltd, Teva Pharmaceuticals, GlaxoSmithKline, Novartis Pharma AG, and Pfizer lnc. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
KEY POINTS
PNECs were previously published for 125 antibiotics; however, it is recognized that this list does not encompass all manufactured antibiotics. A default PNEC value is necessary in order to provide a target for antibiotics with limited data.
Effect of birth ball abdominal core training on pregnancy fatigue, waist pain and delivery outcomes
Authors: Zhang Rong , Xiao Ying , Wei Wei , Wu Bingbing 
Published: November 2021

AbstractObjectiveTo evaluate the effect of birth ball abdominal core training on fatigue, waist pain, and delivery outcomes in middle and late pregnancy.MethodsA prospective study of 209 primipara in total with 24–27 weeks of gestation were recruited and divided into the training group (109 cases) and the control group (100 cases). The control group received routine prenatal exercise. On the basis of routine prenatal exercise, pregnant women in the training group were given birth ball exercises based on abdominal core training. The difference in pregnancy fatigue, waist discomfort, and delivery outcomes between the two groups were compared.ResultsThe waist pain and fatigue ratings of women were significantly lower and the vaginal delivery rate (74.3%) was significantly higher (62%) (P < 0.001) in the training group. There were no significant differences in the total stage of labor, postpartum hemorrhage, neonatal weight and 1‐min neonatal Apgar score between the two groups. The rate of episiotomy in the training group (14.7%) was significantly lower than that in the control group (25.0%) (P < 0.05).ConclusionBirth ball exercises can relieve fatigue and waist pain in middle and late 2021 pregnancy, reduce the rate of episiotomy, and promote spontaneous vaginal delivery.
Natural products against cisplatin‐induced male reproductive toxicity: A comprehensive review
Authors: Rahimi Atena , Asadi Farideh , Rezghi Maedeh , Kazemi Soharb , Soorani Farangiz , Memariani Zahra 
Published: November 2021

AbstractCisplatin is widely used as one of the most effective anticancer agents in the treatment of some neoplasms. Reproductive toxicity is the most common outcome associated with cisplatin testicular damage. Alternative natural medicines for treating male testicular disorders and infertility have received extensive attention in research. Natural products, medicinal herbs, and their secondary metabolites have been shown as promising agents in the management of testicular damage induced by chemotherapy drugs. This study aimed to review the research related to natural substances that are promising in mitigation of the cisplatin‐induced toxicity in the reproductive system. PubMed and Scopus were searched for studies on various natural products for their potential protective property against reproductive toxicity induced by cisplatin from 2000 to 2020. Eligibility was checked based on selection criteria. Fifty‐nine articles were included in this review. Mainly in animal studies, several natural agents have positively affected cisplatin‐reproductive‐toxicity factors, including reactive oxygen species, inflammatory mediators, DNA damage, and activation of the mitochondrial apoptotic pathway. Most of the natural agents were investigated in short‐term duration and high doses of cisplatin exposure, considering their antioxidant activity against oxidative stress. Considering antioxidant properties, various natural products might be effective for the management of cisplatin reproductive toxicity. However, long‐term recovery of spermatogenesis and management of low‐dose‐cisplatin toxicity should be considered as well as the bioavailability of these agents before and after treatment with cisplatin without affecting its anticancer activity.
Successful “on‐demand” plerixafor for autologous peripheral blood stem‐cells transplantation for relapsed/refractory germ cell tumors
Authors: Corbingi Andrea , Metafuni Elisabetta , Di Salvatore Mariantonietta , Putzulu Rossana , Chiusolo Patrizia , Schinzari Giovanni , Massini Giuseppina , Rossi Ernesto , Zini Gina , Cassano Alessandra , Sica Simona , Piccirillo Nicola 
Published: November 2021

AbstractBackgroundGerm cell tumors represent, among solid cancers, a potentially curable disease even if up to 20% to 30% of patients (pts) relapse after first‐line treatment especially considering intermediate and poor prognosis groups. In this scenario, patients are candidates for high‐dose chemotherapy and autologous stem‐cells transplantation as second‐line treatment even though stem‐cells mobilization potential can be affected by several cycles and regimens of chemotherapy. To date, plerixafor is authorized in poor mobilizer adult pts diagnosed with lymphoma or multiple myeloma and in pediatric solid tumors or lymphoma. Therefore, the use of plerixafor in adult pts with relapsing/refractory GCT is still off label.Materials and methodsIn our study, we describe mobilization and collection of peripheral blood stem cells for 10 pts with germ cell tumors. Six patients underwent plerixafor administration since classified as poor mobilizers based on WBC count (>5.000/μL) and CD34+ cell count (<15/μL) the day before apheresis procedure.ResultsOn the first day of apheresis, plerixafor administration in poor mobilizers made possible a remarkable boost of CD34+ cells in such a way to overlap that of good mobilizers' (32/μL vs 35/μL, respectively, P > .05).ConclusionTherefore, in our experience, plerixafor made a good fraction of poor mobilizer patients eligible for mobilization and collection and able to undergo the predicted autologous stem‐cells transplantation; thus, the lack of access to the use of plerixafor in this setting of patients risks jeopardizing an effective treatment, especially in case of poor prognosis.
Identification of novel biomarkers for sepsis diagnosis via serum proteomic analysis using iTRAQ‐2D‐LC‐MS/MS
Authors: Li Meng , Ren Rongrong , Yan Molei , Chen Shangzhong , Chen Chen , Yan Jing 
Published: November 2021

AbstractBackgroundSepsis is a common cause of morbidity and mortality in the ICU patients. Early diagnosis and appropriate patient management is the key to improve the patient survival and to limit disabilities in sepsis patients. This study was aimed to find new diagnostic biomarkers of sepsis.MethodsIn this study, serum proteomic profiles in sepsis patients by iTRAQ2D‐LC‐MS/MS. Thirty seven differentially expressed proteins were identified in patients with sepsis, and six proteins including ApoC3, SERPINA1, VCAM1, B2M, GPX3, and ApoE were selected for further verification by ELISA and immunoturbidimetry in 53 patients of non‐sepsis, 37 patients of sepsis, and 35 patients of septic shock. Descriptive statistics, functional enrichment analysis, and ROC curve analysis were conducted.ResultsThe level of ApoC3 was gradually decreased among non‐sepsis, sepsis, and septic shock groups (p = 0.049). The levels of VCAM1 (p = 0.010), B2M (p = 0.004), and ApoE (p = 0.039) were showing an increased tread in three groups, with the peak values of B2M and ApoE in the sepsis group. ROC curve analysis for septic diagnosis showed that the areas under ROC curve (AUC) of ApoC3, VCAM1, B2M, and ApoE were 0.625, 0.679, 0.581, and 0.619, respectively, which were lower than that of PCT (AUC 0.717) and CRP (AUC 0.706), but there were no significant differences between each index and PCT or CRP. The combination including four validated indexes and two classical infection indexes for septic diagnosis had the highest AUC‐ROC of 0.772.ConclusionProteins of ApoC3, VCAM1, B2M, and ApoE provide a supplement to classical biomarkers for septic diagnosis.
Identifying group‐based patterns of suicidal ideation over the first 10 years after moderate‐to‐severe TBI
Authors: Klyce Daniel W. , Perrin Paul B. , Fisher Lauren B. , Hammond Flora M. , Juengst Shannon B. , Bergquist Thomas F. , Rabinowitz Amanda R. , Wagner Amy K. , Bombardier Charles H. , Niemeier Janet P. , Gary Kelli W. , Giacino Joseph T. , Zafonte Ross D. 
Published: November 2021

AbstractObjectiveTo identify group‐based patterns in suicidal ideation (SI) over the first 10 years after traumatic brain injury (TBI).MethodsParticipants included 9539 individuals in the TBI Model Systems National Database who responded to Patient Health Questionnaire‐9 Item 9 assessing SI at 1, 2, 5, and/or 10 years post‐injury. Ak‐means cluster analysis was conducted to determine group‐based patterns of SI, and pre‐injury variables were compared with ANOVAs and chi‐square tests.ResultsSI and attempts decreased over time. Four group‐based patterns emerged: Low, increasing, moderate, and decreasing SI. The low SI group comprised 89% of the sample, had the highest pre‐injury employment, fewer mental health vulnerabilities, least severe injuries, and were oldest. The increasing SI group had the most severe TBIs, were youngest, and disproportionately Black or Asian/Pacific Islander.ConclusionThese findings reinforce the importance of mental health and suicide risk assessment during chronic recovery from TBI.