bluuebunny/crossref_metadata_2025_split huggingface dataset:
Augmented Reality Technology As A Delivery Mechanism For Psychological Intervention In Adolescents With Asthma: A Randomised Controlled Feasibility Trial Protocol
Authors: Sharrad Kelsey , Martini Caitlin , Kopsaftis Zoe , Tai Andrew , Spurrier Nicola , Smith Ross , Esterman Adrian , Gwilt Ian , Sandford Debra , Carson-Chahhoud Kristin 
Published: December 2021

Abstract
Background: Australia has one of the highest rates of asthma prevalence worldwide, with almost one in 10 children affected. The mental health and wellbeing of asthmatic children is reported to be significantly more impacted than non-asthmatic peers; affecting both asthma management and their overall quality of life. The relationship between asthma and psychological distress is likely bi-directional, therefore requiring an intervention that addresses both psychological and physiological factors. Technology-based psychological interventions provide a potential solution that may increase engagement with treatment amongst adolescents. In particular, augmented reality (AR) is a novel technology that can be tailored to individual populations and has been proven effective in the management of other conditions. No evidence exists currently on the feasibility of AR in the management of psychological wellbeing within the asthmatic community.Methods: An 80-page workbook has been developed based on best-practice asthma guidelines and through consultation with field experts in Psychology and Paediatric Respiratory Medicine, AR tools have been developed following a needs assessment, and a bespoke smartphone app has been developed by Portal Australia. Forty (n=40) young people aged 13-17 years with persistent asthma will be recruited from the Respiratory Department at the Women’s and Children’s Hospital. Participants will be required to download a smartphone application (YOLO de-stress) and will be encouraged to use the app for a month. Participants will be required to complete the Youth Asthma-related Anxiety Scale, Paediatric Asthma Quality of Life Questionnaire and the Asthma Control Questionnaire at baseline and one-month post-intervention. Data analysis will be descriptive, with counts and percentages for categorical data, and means and standard deviations for continuous data compared between baseline and follow-up. For continuous outcome variables mixed effects linear models will be used. For other outcome measures mixed effects generalized linear models will be used. Discussion: This study will explore the feasibility of AR tools to aid delivery of psychological intervention to manage symptoms of elevated psychological distress among young people with asthma, and inform development of a fully powered RCT. Trial registration: This project was prospectively registered with the Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN12620001109998
Nanopore Long-Read Transcriptomics Profiling Reveals Gene Expression Signatures of Mouse Tumor Endothelial Cells 2H-11
Authors: Tian Yuanyuan , Zhao Jiao , Huang Ju , Zhang Haiying , Ni Fushun , Tang Rong , Shao Jing , Li Yilei , Li Wei 
Published: December 2021

Abstract
Background:Tumor endothelial cells (TECs) play an indispensable role in tumor growth and metastasis. Compared with normal endothelial cells (NECs), TECs exhibit unique phenotypic and functional heterogeneity in terms of metabolism, genetics, and transcriptomics. It is not only the key to coordinate tumor angiogenesis, but also an important factor of immune regulation in the tumor microenvironment. In recent years, the role of TECs in tumor metabolism and invasion has been continuously reported. However, the research on the mechanism behind the complex functions of TECs is still at the basic stage. We use Oxford Nanopore Technology (ONT) three-generation full-length transcriptome sequencing to detect all genetic structural changes in the transcriptome of mouse TECs 2H-11 and mouse NECs SVEC4-10.Results: In Tumor endothelial cells 2H-11,1847genes are up-regulated and 1202 genes are down-regulated. According to the Gene ontology (GO) enrichment analysis of differentially expressed genes (DEGs), we found that different functional trends related to metabolic processes, developmental processes, localization, immune system processes, and locomotion are the main reasons for the differences. DEGs are mainly enriched in signal pathways related to cancer, immunity and metabolism, involving Pathways in cancer,Antigen processing and presentation , Proteoglycans in cancer, Focal adhesion, MAPK signaling pathway ,Protein digestion and absorption，ECM-receptor interaction，PI3K-Akt signaling pathway and Glutathione metabolism. We also obtained the structural variation of transcripts such as alternative splicing, gene fusion, and alternative polyadenylation and accurately quantified the expression of the transcript. Some of our results have been confirmed in other documents. But other data have not been reported yet, which is the focus of our future exploration.Conclusion: We try to use transcriptomics and bioinformatics methods to characterize tumor endothelial cell-related genes and signaling pathways.It could help better understand the molecular mechanisms of tumor endothelial cells involved in tumorigenesis and development. DEGs in key pathways may be potential diagnostic markers or therapeutic targets of TECs. Our data also provide useful genetic resources for improving the genome and transcriptome annotations of TECs and NECs.
Overall Survival Rate Analysis For Risk Factors In Women With Neuroendocrine Carcinoma of The Cervix Using Surveillance, Epidemiology, And End Results (SEER) Database
Authors: Yin Ziran , Huang Xiumin 
Published: December 2021

Abstract
Background: Neuroendocrine carcinoma of the cervix is rare and aggressive disease, of which prognosis information and the effectiveness of the therapies is unclear.Methods: A retrospective study using data from the SEER database for the first diagnosed Neuroendocrine carcinoma of the cervix patients was conducted. We performed univariate and multivariate Cox models to screen for independent prognostic factors for overall survival. Subgroup analysis and sensitive analysis were performed for further study, then again univariate and multivariate analyses of Cox regression analysis were performed based on the sensitivity analysis data set.Results: A total of 250 Neuroendocrine carcinoma of the cervix cases was included, tumor subtype, age, marriage, race, number of regional lymph nodes, number of positive lymph nodes, radiotherapy, surgery, and FIGO stage were all factors affecting OS, and multivariate analysis identified FIGO staging (HR, 2.4; 95% CI, 1.505-3.828, P < 0.001) and surgery (HR, 0.467; 95% CI, 0.358-0.609, P < 0.001) treatment as independent indicators. With respect to the factors associated with treatments, we found that patients who underwent surgery (yes vs. no vs. unknown) or radiation (yes vs. no) experienced prolonged survival, both P < 0.001Conclusions: Our investigation shows that for patients with NECC surgery seems to be the effective treatment. Chemotherapy cannot improve the prognosis of NECC patients, and the effectiveness of radiation should be further verified.
Antioxidant Potentials of the Pod Extract of Caesalpinia Pulcherrima Swarta (Fabaece) and the Theoretical Evaluation of the Antioxidant Property of the Isolated Compounds
Authors: Faloye Kolade , Famuyiwa Samson , Akinwunmi Kemi , Tata Charlotte , Ayoola Marcus , Fakola Emmanuel , Akinyele Olawale , Ndinteh Derek 
Published: December 2021

Abstract
Various diseases associated with oxidative stress have necessitated the need to investigate plants for antioxidant agents. This study aims to investigate the antioxidant potential of the extract and fractions of C. pulcherrima and estimate the quantum chemical properties of polyphenolic compounds isolated from its most active antioxidant fraction. Methanolic extract and partition fractions of the pods of the plant were assayed for their antioxidant activity using four models: 1,1-diphenyl-2-dipicrylhydrazyl radical scavenging, ferric reducing antioxidant power, total antioxidant capacity and hydroxyl radical scavenging activity. Total phenolic and flavonoid contents of the extract and fractions were also investigated. Furthermore, the quantum chemical properties of two polyphenolic compounds were calculated to predict the antioxidant potential. The extract exhibited good antioxidant activity with IC50 = 45.63 µg/ml comparable to ascorbic acid of IC50 = 37.94 µg/ml, high reducing power with value at 376.74 ± 6.78, total antioxidant capacity obtained at ascorbic acid equivalence of 383 ± 8.16 mgAAE/g and potent hydroxyl radical scavenging activity with value at 580.97 µg/ml with ascorbic acid at 30.7 µg/ml. The total phenol and flavonoid contents values at 540.53 ± 3.71 and 347.87 ± 7.13 respectively. Furthermore, the results obtained from the quantum chemical calculations reveals polyphenolic compounds; 5(4-hydroxyphenyl)-3-hydroxy-2-methoxyphenol and 3(4-methanetriiol-2,6-dihydroxyphenoxy)-3’, 4’, 5’, 5, 7-pentahydroxyflavonol as promising antioxidant agents. The study concluded that C. pulcherrima pods have good antioxidant activity. Also, the polyphenolic compounds are among the chemical constituents responsible for the antioxidant activity obtained for the extract and ethyl acetate fraction.
Effects of BET Inhibitor JQ1 and Interleukin-6 on Breast Cancer Cells
Authors: Hoseini Atefeh Sharif , Heshmati Masoud , Soltani Amin , Shirzad Hedayatollah , Sedehi Morteza , Judd Barbi A , Jami Mohammad-Saeid , Samani Mahdi Ghatreh 
Published: December 2021

Abstract
Bromodomain and extra-terminal (BET) proteins are recognized acetylated lysine of histone 4 and act as scaffolds to recruit many other proteins to promoters and at enhancers of active genes, especially at the super-enhancers of key genes, driving the transcription process and have been identified as potential therapeutic targets in breast cancer. However, the efficacy of BET inhibitors such as JQ1 in breast cancer therapy is impeded by IL-6 through an as yet defined mechanism. We investigated the interplay between IL-6 and JQ1 in MCF-7 and MDA-MB-231 human breast cancer cells. Here we demonstrate that the efficacy of JQ1 on the inhibition of cell growth and apoptosis was stronger in MDA-MB-231 cells than in MCF-7 cells. Further, MCF-7 cells, but not MDA-MB-231 cells, exhibited increased expression of CXCR4 following IL-6 treatment. JQ1 significantly reduced CXCR4 surface expression in both cell lines and diminished the effects of IL-6 pre-treatment on MCF-7 cells. While IL-6 suppressed the extension of breast cancer stem cells (BCSCs) in MCF-7 cells, JQ1 impeded its inhibitory effect. In addition, in MCF-7 cells JQ1 increased the number of senescent cells in a time-dependent manner. Analysis of gene expression indicated that JQ1 and IL-6 synergistically increase SNAIL expression and decrease c-MYC expression in MCF-7 cells. So, the BET proteins are promising, novel therapeutic targets in late-stage breast cancers.
Evaluating Palin Stammering Therapy for School Children (Palin STSC 8-14): Protocol for a Feasibility Randomised Control Trial Comparing Palin STSC(8-14) with Usual Treatment
Authors: Millard Sharon K , Murphy Suzanne , Barton Garry , Lethersich Maria , Rixon Lorna , Shepstone Lee , Sims Erika , Joffe Victoria 
Published: December 2021

Abstract
Background: Having a stammer can have a significant effect on a child’s social, emotional and educational development. With approximately 66,000 children in the UK having a stammer, there is a need to establish an adequate evidence base to inform clinical practise. We describe a feasibility trial to explore the effectiveness of a new therapy programme for children aged 8-14: Palin Stammering Therapy for School Children (Palin STSC(8-14)). Preliminary data from the Michael Palin Centre, where the programme was developed, indicate that Palin STSC(8-14) is effective in reducing stammering frequency and impact for children, with beneficial effects for parents too. We will investigate the feasibility of the methods required for a definitive randomised control trial to investigate the application of this therapy by NHS speech and language therapists (SLTs), compared with ‘treatment as usual’ (TAU), beyond the specialist context in which it was developed.Methods: This is a two-arm feasibility cluster-randomised controlled trial of Palin STSC(8-14) with TAU control arm, and randomisation at the level of the SLT. Quantitative and qualitative data will be collected to examine: the recruitment and retention of therapists and families; the acceptability of the research processes and the therapeutic intervention; the appropriateness of the therapy outcome measures. Assessments will be completed by children and parents at baseline and six months later, including measures of: stammering severity; the impact of child’s stammering on both children and parents; child temperament, behaviour, peer relations, anxiety; quality of life; and, economic outcomes. There will also be a qualitative process evaluation, including interviews with parents, children, SLTs and SLT managers to explore the acceptability of both the research and therapy methods. Treatment fidelity will be examined through analysis of therapy session records and recordings. Discussion: The findings of this feasibility trial will inform the decision as to whether to progress to a full-scale randomised controlled trial to explore the effectiveness of Palin STSC(8-14) when compared to Treatment as Usual in NHS SLT services. There is a strong need for an evidence based intervention for school age children who stammer. Trial registration: ISRCTN. ISRCTN17058884. Registered 18th December 2019. https://www.isrctn.com/ISRCTN17058884
A Spatial AMH Copula-Based Dissimilarity Measure to Cluster Variables in Panel Data
Authors: Lascio F. Marta L. Di , Menapace Andrea , Pappadà Roberta 
Published: December 2021

Abstract
Investigating thermal energy demand is crucial for the development of sustainable cities and efficient use of renewable sources. Despite the advances made in this field, the analysis of energy data provided by smart grids is currently a demanding challenge. In this paper, we develop a clustering methodology based on a novel dissimilarity measure to analyze a high temporal resolution panel data for district heating demand in the Italian city Bozen-Bolzano. Starting from the characteristics of this data, we explore the usefulness of the Ali-Mikhail-Haq copula in defining a new dissimilarity measure to cluster variables in a hierarchical framework. We show that our proposal is particularly sensitive to small dissimilarities based on tiny differences in the dependence level. Therefore, the proposed measure is able to better distinguish between objects with low dissimilarity than classic rank-based dissimilarity measures. Moreover, our proposal is defined in a spatial version that is able to take into account the spatial location of the compared objects. We investigate the proposed measure through Monte Carlo studies and compare it with the corresponding spatial Kendall's correlation-based dissimilarity measure. Finally, the application to real data makes it possible to find clusters of buildings homogeneous with respect to their main characteristics, such as energy efficiency and heating surface, to support the design, expansion and management of district heating systems.
Melatonin Modulates Microglia Activation in Neuro-inflammation by Regulating the ER Stress/PPARd/SIRT1 Signaling Pathway
Authors: Pan Hung-Chuan , Yang Cheng-Ning , Lee Wen-Jane , Sheehan Jason , Wu Sheng-Mao , Chen Hong-Shiu , Lin Mao-Hsun , Shen Li-Wei , Lee Shu-Hua , Shen Chin-Chang , Pan Liang-Yi , Sheu Meei-Ling 
Published: December 2021

Abstract
Background: Activated microglia-mediated neuro-inflammation plays a vital aspect in regulating the micromilieu of the central nervous system. Neuro-inflammation involves distinct alterations of microglial phenotypes, containing nocuous pro-inflammatory (M1) phenotype and neuroprotective anti-inflammatory (M2) phenotype. Currently, there is no effective treatment for modulating such alterations. Little evidence shows that melatonin prevents the detrimental cascade of activated microglia-mediated neuro-inflammation. Methods: The expression levels of M1/M2 marker of primary microglia influenced by Melatonin were detected via qPCR. Functional activities were explored by western blotting, luciferase activity, EMSA, and ChIP assay. Structure interaction was assessed by molecular docking and LIGPLOT analysis. ER stress detection was examined by ultrastructure TEM, calapin activity, and ERSE assay. The neurobehavioral evaluations and immunofluorescence staining in animals were used for investigation of Melatonin on the neuroinflammation in vivo. Results: Melatonin had targeted on Peroxisome Proliferator Activated Receptor Delta (PPARd) activity, boosted LPS-stimulated alterations in polarization from the M1 to the M2 phenotype, and thereby inhibited NFkB–IKKb activation in primary microglia. The PPARd agonist L-165041 or over-expression of PPARd plasmid (ov-PPARd) showed similar results. Molecular docking screening, dynamic simulation approaches, and biological studies of melatonin showed that the activated site was located at PPARd (phospho-Thr256-PPARd). Furthermore, we found that activated microglia had lowered PPARd activity as well as the downstream SIRT1 formation via enhancing ER stress. Melatonin, PPARd agonist and ov-PPARd all effectively reversed the above-mentioned effects. Melatonin blocked ER stress by regulating calapin activity and expression in LPS-activated microglia. Additionally, melatonin or L-165041 ameliorated the neurobehavioral deficits in LPS-aggravated neuroinflammatory mice through blocking microglia activities, and also promoted phenotype changes to M2-predominant microglia. Conclusions Melatonin suppressed neuro-inflammation in vitro and in vivo by tuning microglial activation through the ER stress-dependent PPARd/SIRT1 signaling cascade. We proposed that this treatment strategy is an encouraging pharmacological approach for the remedy of neuro-inflammation associated disorders.
Fracture Resistance of Primary Canines Restorations Reinforced with a Flowable or a Packable Composite Resin Posts; In- Vitro
Authors: Ansari Ghasem , javadi Fatemeh , Hanjani Kiana , Abrandabadi Ahmad Najafi , Gashtasb Mahshid , Roodgarian Romina 
Published: December 2021

Abstract
Aim and Background:
Restoration of severely damaged primary anterior teeth are deemed as serious challenges for pediatric dentists. The aim of this study was to evaluate the fracture resistance of primary canines restored with flowable or packable composite resin posts.
Material and Methods
This study was conducted on a group of extracted primary canines. After cutting the coronal section, standard pulpectomy was performed and root canals were filled with ZOE paste leaving 3 mm coronal root space. A liner was placed above the root canal filling as a barrier. Samples were then randomly divided into two groups of fifteen. Both groups, received etch, bond, then in group I Core Flo Composite resin placement into canal, in group II, post shaping using designated packable composite resin and inserted into the canal. Followed by Core buildup and Crown reconstruction using Z250 composite resin. Samples were mounted at 148 ° angle on the acrylic blocks and stored in de-ionized water for 72 hours. Fracture resistance was measured using Universal Testing Machine. Data analysis were performed using independent t-test. Chi- square test were also employed to assess the type of fracture within groups.
Results
The mean fracture resistance in the group I was 830.31(+/-328.69N) and 803.09(+/-326.45N) in group II. Moreover, statistical analyses of the results showed no significant difference between the mean values of the fracture resistance (p-value=0.822) of groups as well as the type of fracture (p-value=0.79) between groups.
Conclusion
The ease of use and proper adhesion of the Core Flo composite resin, seems to help its use in the reconstruction of severely destructed anterior primary teeth.
The Role of Service Provider’s Rapport Behavior on Customers’ Empathy
Authors: Jin ChangHyun , Zeng JingYun 
Published: December 2021

Abstract
The study is intended to explore the validity of the component system of the rapport behavior of service providers. The purpose of this study was to understand how the components of the rapport behavior of service providers affect rational and emotional empathy. This study is intended to understand the relationship between rational and emotional empathy and customer satisfaction and the effect of customer satisfaction on revisit intentions. The main purpose was to identify the moderating effect of perceived authenticity on the causal relationship between rapport behavior, empathy, and customer satisfaction and revisit intentions. The sampling method used in this study was the judgment sampling, which is a method in which the researcher selects those study subjects that are thought by the researcher to be the most suitable samples for the purpose of the study. The total questionnaire period was a total of three months, from March 15 to May 15, 2020. The total number of valid samples was 1668. Uncommonly attentive behavior, courteous behavior, information-sharing, and connecting behavior, which are components of the rapport behaviors of service providers, were found to have positive effects on rational empathy with the service provider, but common grounding behavior, which is also a component of the rapport behaviors of service providers, was found to have no statistically significant effect on rational empathy with service providers. The rapport behaviors of service providers are judged to play an important role in maintaining and developing strong ties through long-term and continuous interactions with customers.
Effects of Sarcopenia and Sarcopenic Obesity on Joint Pain and Degenerative Osteoarthritis in Postmenopausal Women
Authors: Kim Hye In , Ahn So Hyun , Kim Yup , Lee Ji Eun , Seo Seok Kyo 
Published: December 2021

Abstract
This study aimed to identify the prevalence of sarcopenia, obesity, and sarcopenic obesity and examine their association with radiographic knee osteoarthritis (OA) and knee pain in Korean menopausal women. This cross-sectional study utilized the data from Korean National Health and Nutrition Examination Surveys 2009–2011. The participants were categorized based on body composition. The prevalence of radiographic knee OA and knee pain was calculated. The effect of hormone therapy (HT) was also evaluated. The prevalence of radiographic knee OA, knee pain, and both were all highest in the sarcopenic obese group and lowest in the non-sarcopenic non-obese group. Without sarcopenia, obese women showed higher ratio of radiographic knee OA. With sarcopenia, the coexistence of obesity presented higher ratio of radiographic knee OA, knee pain, and both compared to sarcopenia without obesity. The use of HT for more than one year was not associated with radiographic knee OA, knee pain, or both. Obesity with sarcopenia had greater effect on knee OA compared to obesity without sarcopenia. Moreover, HT use for more than one year was not associated with the prevalence of knee OA. Therefore, more efforts should focus on reducing body fat and increasing muscle in postmenopausal women with knee OA.