bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of vestibular system in profound sensorineural hearing loss patients
Authors: Al-Ghandour Faten Magdy , Nada Nashwa Mohammed Fareed , Kolkaila Enaas Ahmad , Emara Afaf Ahmad , Essawy Wessam Mostafa 
Published: December 2024

Abstract
Background
The cochlea and vestibule share a continuous membranous structure and similar receptor cell ultrastructures and are supplied by common arterial blood. Therefore, inner ear diseases probably affect the cochlea as well as the vestibular system.

Purpose
This study aimed to evaluate vestibular system in profound sensorineural hearing loss patients.

Methods
This work included 40 adults. They were divided into 20 normal hearing adults who had no vestibular complaint (control group) and 20 profound sensorineural hearing loss (PSNHL) adults who had no vestibular complaint (study group). They were evaluated by cervical vestibular-evoked myogenic potentials (cVEMPs), ocular vestibular-evoked myogenic potentials (oVEMPs), subjective visual vertical (SVV), subjective visual horizontal (SVH), and video head impulse test (vHIT).

Results
cVEMPs were successfully recorded from all subjects in control group, while it was absent in 37.5% of the study group. oVEMPs showed normal response in control group, while it was absent in 60% of the study group. Subjects in the control group showed normal SVV/H values, while in study group SVV showed abnormal values in 30% and SVH showed abnormal values in 35%. Subjects in the control group showed normal vHIT values, while in study group vHIT showed abnormal values in (55%).

Conclusions
In PSNHL patients, inner ear diseases affected both the vestibular system and the cochlea. This was evidenced by the high prevalence of absent oVEMPs and cVEMPs as well as abnormal values of SVV, SVH, and vHIT in the study group.
The impact of Coblation- assisted endoscopic DRAF II procedures in the treatment of chronic frontal sinus disease
Authors: Sebastian Susan K. , Arora Gautamkumar 
Published: December 2024

Abstract
Background
To evaluate the surgical outcome following Coblation-assisted Draf II procedures in chronic frontal sinus disease.

Materials and methods
A retrospective observational study was done on a series of 31 patients who underwent endoscopic Coblation-assisted DrafIIa and IIb procedures from October 2021 to September 2023 at a tertiary care hospital. Subjective improvement was assessed using SNOT-22 scores. Objective evaluation was done using nasal endoscopy scores.

Results
The SNOT-22 score showed a significant reduction (p value -.0001) following the procedure, indicating improvement in symptoms and quality of life. The endoscopic score also demonstrated a significant reduction (p value.0001) following the procedure. A positive correlation was also observed between SNOT-22 and endoscopic scores. The proportion of patients with postoperative complications was low in the present study in comparison to several previous studies.

Conclusions
Coblation-assisted Draf II procedures are an effective treatment choice in the management of chronic frontal sinus disease. It provides safe access to the frontal sinus, leading to better frontal sinus patency and an improvement in the quality of life of the patients.
Triangulating evidence from the GALENOS living systematic review on trace amine-associated receptor 1 (TAAR1) agonists in psychosis
Authors: Smith Katharine A. , Boyce Niall , Chevance Astrid , Chiocchia Virginia , Correll Christoph U. , Donoghue Kim , Ghodke Nikita , Kambeu Tatenda , Malhi Gin S. , Macleod Malcolm , Milligan Lea , Morgan Jamie , Potts Jennifer , Robinson Emma S. J. , Siafis Spyridon , Sommer Iris E. C. , Voelkl Bernhard , Salanti Georgia , Cipriani Andrea , Higgins Julian P. T. 
Published: December 2024

Background
Trace amine-associated receptor 1 (TAAR1) agonists offer a new approach, but there is uncertainty regarding their effects, exact mechanism of action and potential role in treating psychosis.

Aims
To evaluate the available evidence on TAAR1 agonists in psychosis, using triangulation of the output of living systematic reviews (LSRs) of animal and human studies, and provide recommendations for future research prioritisation.

Method
This study is part of GALENOS (Global Alliance for Living Evidence on aNxiety, depressiOn and pSychosis). In the triangulation process, a multidisciplinary group of experts, including those with lived experience, met and appraised the first co-produced living systematic reviews from GALENOS, on TAAR1 agonists.

Results
The animal data suggested a potential antipsychotic effect, as TAAR1 agonists reduced locomotor activity induced by pro-psychotic drug treatment. Human studies showed few differences for ulotaront and ralmitaront compared with placebo in improving overall symptoms in adults with acute schizophrenia (four studies, n = 1291 participants, standardised mean difference (SMD) 0.15, 95% CI −0.05 to 0.34). Large placebo responses were seen in ulotaront phase three trials. Ralmitaront was less efficacious than risperidone (one study, n = 156 participants, SMD = −0.53, 95% CI −0.86 to −0.20). The side-effect profile of TAAR1 agonists was favourable compared with existing antipsychotics. Priorities for future studies included (a) using different animal models of psychosis with greater translational validity; (b) animal and human studies with wider outcomes including cognitive and affective symptoms and (c) mechanistic studies and investigations of other potential applications, such as adjunctive treatments and long-term outcomes. Recommendations for future iterations of the LSRs included (a) meta-analysis of individual human participant data, (b) including studies that used different methodologies and (c) assessing other disorders and symptoms.

Conclusions
This co-produced, international triangulation examined the available evidence and developed recommendations for future research and clinical applications for TAAR1 agonists in psychosis. Broader challenges included difficulties in assessing the risk of bias, reproducibility, translation and interpretability of animal models to clinical outcomes, and a lack of individual and clinical characteristics in the human data. The research will inform a separate, independent prioritisation process, led by lived experience experts, to prioritise directions for future research.
Runx3, Brn3a and Isl1 interplay orchestrates the transcriptional program in the early stages of proprioceptive neuron development
Authors: Orlovsky Kira , Appel Elena , Hantisteanu Shay , Olender Tsviya , Lotem Joseph , Levanon Ditsa , Groner Yoram 
Published: December 2024

Background
The development and diversification of sensory proprioceptive neurons, which reside in the dorsal root ganglia (DRG) and express the tropomyosin receptor kinase C (TrkC), depend on the transcription factor (TF) Runx3. Runx3-deficient mice develop severe limb ataxia due to TrkC neuron cell death. Two additional TFs Pou4f1 (also called Brn3a) and Isl1 also play an important role in sensory neuron development. Thus, we aimed to unravel the chromatin state of early-developing TrkC neurons and decipher the Runx3 high-confidence target genes (HCT) and the possible cooperation between Runx3, Brn3a and Isl1 in the regulation of these genes.

Methods
Runx3 expression is driven by the gene proximal P2 promoter. Transcriptome analysis was conducted by RNA-seq on RNA isolated from heterozygous (P2+/-) vs. homozygous (P2-/-) TrkC neurons and differentially expressed genes (DEGs) were determined. Genome-wide occupancy of Runx3, Brn3a, Isl1 and histone H3 acetylated on lysine 27 (H3K27Ac) was determined using CUT&RUN. The landscape of Transposase-accessible chromatin was analyzed via ATAC-seq.

Findings
The intersection of Runx3 genomic occupancy-associated genes and DEG data discovered 244 Runx3 HCT. Brn3a and Isl1 were found to bind to numerous genomic loci, some of which overlapped with Runx3. Most genomic regions bound by each of these three TFs or co-bound by them resided in distantly located enhancer regions rather than in gene promoters. In activated and suppressed neuronal Runx3 HCT, Runx3 cooperated mainly with Brn3a to regulate expression through distantly located enhancers. Interestingly, suppression of non-neuronal immune genes was mainly managed via Runx3 without Brn3a. The distribution of ATAC and H3K27Ac marked regions in Runx3 peaks containing at least one RUNX binding site (Runx3_RBS) revealed that while most promoter regions were marked by ATAC, a prominent fraction of intron/intergenic regions occupied by Runx3, Brn3a or Isl1 were unmarked by ATAC and/or H3K27Ac.

Conclusions
These analyses shed new light on the interplay of Runx3, Brn3a, Isl1, and open chromatin regions in regulating the Runx3 HCT in the early developmental stages of TrkC neurons.
Primipaternity in multiparas as a predominant high risk factor for preeclampsia over prolonged birth intervals: A study of 33,000 singleton pregnancies in Reunion Island
Authors: Robillard Pierre-Yves , Iacobelli Silvia , Lorrain Simon , Bonsante Francesco , Boukerrou Malik , Scioscia Marco , Tran Phuong Lien , Dekker Gustaaf 
Published: December 2024

Objectives
To evaluate the relative importance of changing paternity (“primipaternity”, direct inquiry with patients) in multiparas versus prolonged birth/pregnancy interval as risk factors for preeclampsia (PE) by a logistic regression model comparing the adjusted odds ratios of both exposures.

Design
Assessment of all consecutive singleton deliveries (from 22 weeks onwards) at South-Reunion University’s maternity (Reunion Island, Indian Ocean) over 23 years (2001–2023) using an epidemiological perinatal database on obstetrical factors (264 items in total, of which, chronic or gestational hypertension, proteinuria, HELLP syndrome).

Results
Among the 53,572 multiparous singleton pregnancies, we identified 33,312 (62%) of multiparas who gave consecutive births, allowing calculation of birth intervals.
Primipaternity multipara (N = 2790) were on average older than those in stable relationships (N = 50,782), 31 vs 30 years, p< 0.0001; they had almost systematically longer birth intervals compared with controls of approximately 1.5 year from the 2nd to the 4th pregnancy and approximately 1year after the 5th pregnancy (all p < 0.05). In the logistic regression model of 11 risk factors, intervals between pregnancies had similar adjusted odds ratios (1.05, p = 0.002) as increasing maternal age (AdjOR 1.02, p = 0.02), increasing parity (adjOR 1.09, p = 0.02) and pre-pregnancy BMI (AdjOR 1.05, p< 0.0001). Smoking was associated with an AdjOR of 0.85 (non-significant),primipaternity multiparas were twice as likely to be smokers (23.8% vs 13.4%, p< 0.0001) compared with controls. AdjOR for primipaternity was 3.34 (p < 0.0001) indicating that primipaternity as risk belonged in the category of well-established risk factors like history of preeclampsia (11.2, p< 0.0001) and chronic hypertension (6.45, p< 0.0001).

Conclusions
Primipaternities in multiparae belongs to the major risk factors such as history of preeclampsia, chronic hypertension, multiple pregnancies while prolonged birth intervals belongs to moderate “regular physiological aging processes” such as increasing maternal age, parity or increasing pre-pregnancy BMI.
DMRIntTk: Integrating different DMR sets based on density peak clustering
Authors: Zhang Wenjin , Jie Wenlong , Cui Wanxin , Duan Guihua , Zou You , Peng Xiaoqing 
Published: December 2024

Background
Identifying differentially methylated regions (DMRs) is a basic task in DNA methylation analysis. However, due to the different strategies adopted, different DMR sets will be predicted on the same dataset, which poses a challenge in selecting a reliable and comprehensive DMR set for downstream analysis.

Results
Here, we develop DMRIntTk, a toolkit for integrating DMR sets predicted by different methods on a same dataset. In DMRIntTk, the genome is segmented into bins, and the reliability of each DMR set at different methylation thresholds is evaluated. Then, the bins are weighted based on the covered DMR sets and integrated into final DMRs using a density peak clustering algorithm. To demonstrate the practicality of DMRIntTk, it was applied to different scenarios, including tissues with relatively large methylation differences, cancer tissues versus normal tissues with medium methylation differences, and disease tissues versus normal tissues with subtle methylation differences. Our results show that DMRIntTk can effectively trim regions with small methylation differences from the original DMR sets and thereby enriching the proportion of DMRs with larger methylation differences. In addition, the overlap analysis suggests that the integrated DMR sets are quite comprehensive, and functional analyses indicate the integrated disease-related DMRs are significantly enriched in biological pathways associated with the pathological mechanisms of the diseases. A comparative analysis of the integrated DMR set versus each original DMR set further highlights the superiority of DMRIntTk, demonstrating the unique biological insights it can provide.

Conclusions
Conclusively, DMRIntTk can help researchers obtain a reliable and comprehensive DMR set from many prediction methods.
ATRA-induced NEAT1 upregulation promotes autophagy during APL cell granulocytic differentiation
Authors: Tang Doudou , Wang Huihui , Jiang Yafeng , Chen Mingjie , Zhang Guangsen , Wu Shangjie , Wang Yewei 
Published: December 2024

Aims
Acute promyelocytic leukemia (APL) progresses quickly and often leads to early hemorrhagic death. Treatment with all-trans retinoic acid (ATRA) promotes differentiation of APL cells and clinical remission, making APL a potentially curable malignancy. Understanding how ATRA works may lead to new treatments for other types of leukemia. Long non-coding RNA NEAT1 has been implicated in the differentiation of APL cells. This study aims to elucidate the specific role of NEAT1 in the granulocytic differentiation of APL.

Methods
The influence of NEAT1 on autophagy and PML/RARα degradation was assessed using western blot assays. The impact of NEAT1 on the expression of autophagy-related genes was evaluated through quantitative real-time RT-PCR. Mechanistic insights into the role of NEAT1 in modulating autophagy were supported by RNA immunoprecipitation and RNA pulldown assays.

Key findings
Knockdown of NEAT1 suppressed autophagy and attenuated ATRA-induced PML/RARα degradation and granulocytic differentiation of APL cells. Subsequent screening of autophagy-related genes demonstrated that silencing NEAT1 impaired the ATRA-induced upregulation of ATG10 and ATG12. Mechanistic investigations revealed that the RNA-binding protein TAF15 interacted with NEAT1, synergistically stabilizing the mRNA of ATG10 and ATG12. Furthermore, knockdown of NEAT1 impaired the interactions between TAF15 and the mRNAs of ATG10 and ATG12, thereby compromising their mRNA stability.

Significance
Our study elucidates the critical role of NEAT1-mediated autophagy in the differentiation of APL cells and delineates the molecular mechanism by which upregulation of NEAT1 enhances autophagy. Specifically, NEAT1 binds to the RNA-binding protein TAF15, which in turn stabilizes the mRNA of both ATG10 and ATG12.
A systems biology-based study to assess the effects of TNF-alpha ± apigenin in triple-negative breast cancer cell line
Authors: Amjad E , Sokouti B , Asnaashari S , Dastmalchi S 
Published: December 2024

Triple-negative breast cancer (TNBC) is a type of breast cancer that lacks estrogen, progesterone, and HER2 receptors. Various treatment methods are available for breast cancer, but therapies with minimal toxic side effects are particularly important. This study computationally investigates the impact of apigenin, a compound used in traditional Chinese medicine, on the TNBC cell line. The GSE120550 dataset was retrieved from the NCBI-GEO database. BRB-ArrayTools were used for pre- and post-processing to identify significantly differentially expressed genes. Additionally, the DAVID web server was utilized to analyze three main components:"biological process," "cellular component," and "molecular function," along with the KEGG signaling pathway. Finally, a Venn diagram was employed to thoroughly investigate the number of shared genes among 15 groups derived from 6 compared sample sets. The primary analysis of 6 pairs of samples revealed significant differentially expressed genes (DEGs), which were prioritized using the TOPPgene web server. These identified genes, playing key roles in inhibiting the progression of BC, are involved in various signaling pathways. Protein-protein interaction network analysis highlighted the biomarkers associated with the inhibitory effects of apigenin across the 15 sets derived from the 6 sample pairs. The findings of this study confirm the inhibitory effects of apigenin, with no toxic side effects, on patients with TNBC. This natural compound holds promise for future therapeutics and novel drug designs.
Crystal structure of 14-(R)-(2′-cyano-phenoxy)-3,19-diacetyl andrographolide, C31H37NO7
Authors: Liu Zhuyun , Ding Pengfei , Yang Shanshan , Zhai Donghua , Sha Yunying , Zhang Ruifang , Wang Lizhong 
Published: December 2024

Abstract
C31H37NO7, monoclinic, P21 (no. 4), a = 8.1269(1) Å, b = 11.0527(1) Å, c = 16.3586(2) Å, β = 97.973(1)°, V = 1455.19(3) Å3, Z = 2, Rgt
(F) = 0.0467, wRref
(F
2) = 0.1308, T = 293(2) K.
Crystal structure of poly[diaqua-bis(μ2-5-((pyridin-4-yl-methyl)amino)benzene-1,3-dicarboxylato-κ
2
N:O)cadmium(II)], C28H26CdN4O10
Authors: Zhang Lai-Jun , Weng Wen-Fang , Huang Shuai , Ye Xiao-Qing , Deng Yuan-Yuan , Lang Jia-Yi , Yin Qing , Ding Jun-Jie 
Published: December 2024

Abstract
C28H26CdN4O10, triclinic,

P

1
‾

$P\overline{1}$

(no. 2), a = 5.7700(12) Å, b = 10.190(2) Å, c = 12.160(2) Å, α = 70.38(3)°, β = 84.72(3)°, γ = 81.63(3)°, V = 665.6(3) Å3, Z = 1, R

gt
(F) = 0.0272, wR

ref
(F
2) = 0.0602, T = 293 K.
Conduction transportation from graphene to an insulative polymer medium: A novel approach for the conductivity of nanocomposites
Authors: Zare Yasser , Munir Muhammad Tajammal , Rhee Kyong Yop , Park Soo-Jin 
Published: December 2024

Abstract
Some models have been proposed for the electrical conductivity of graphene-filled nanocomposites, but they have not reflected the characteristics of an imperfect interphase surrounding the graphene nanosheets. In this article, the size and conductivity of an imperfect interphase are used to develop a model for conductivity of the graphene/polymer system. Also, “Y,” the degree of conduction transfer through an imperfect interphase, is expressed as graphene dimensions and interphase conductivity to define the effective converse aspect ratio and effective filler portion in the samples. The developed model for nanocomposite conductivity is examined by the experimental data of some samples. Furthermore, the influences of various factors on “Y,” percolation onset, and nanocomposite conductivity are investigated. Thin and large nanosheets, poor filler conductivity, and high interphase conductivity produce a high “Y.” Likewise, “Y” and graphene volume portion

(

φ

f

)

({\varphi }_{\text{f}})

significantly govern the conductivity of samples. Y = 9 and

φ

f

{\varphi }_{\text{f}}

= 0.03 yield the highest nanocomposite conductivity of 16 S/m, while Y < 2 or

φ

f

{\varphi }_{\text{f}}

< 0.022 cannot improve the conductivity of samples.
Combining the Albumin-to-fibrinogen ratio and pathologic factors predicts survival in surgically treated patients with esophageal squamous cell carcinoma
Authors: Zhang Xueshun , Li Xinyu , Xing Lei , Ren Ping 
Published: December 2024

Abstract

Objectives
The primary objectives of this research were to examine the prognostic significance of the albumin-to-fibrinogen ratio (AFR) in patients who have undergone surgery for esophageal squamous cell carcinoma (ESCC) and to develop an easily implementable predictive model with clinical utility.

Methods
The present study retrospectively analyzed the clinical data of 414 patients who underwent R0 resection after being diagnosed with stage I–III ESCC. The prognostic value of AFR was evaluated using Kaplan-Meier survival curves and COX proportional risk regression modeling, and the effectiveness of AFR compared with other inflammatory markers was evaluated. Additionally, a nomogram prediction model was developed, and its accuracy was evaluated using the receiver operating characteristic (ROC) curves, decision curve analysis (DCA), and calibration curves.

Results
AFR was significantly correlated with tumor length, T-stage, N-stage, pathological stage, and vascular infiltration (p<0.05 for all). The multivariate analysis results demonstrated that AFR was an independent prognostic factor that affected patient outcomes, whereas other inflammatory and nutritional biomarkers did not. Furthermore, the overall C-index of the nomogram risk prediction model was 0.737 (95 %-CI: 0.700–0.776). The calibration curves showed that the 3- and 5-year overall survival (OS) probabilities predicted by the nomogram were consistent with actual observations. Moreover, the DCA and ROC curves showed that our model had better clinical utility.

Conclusions
Preoperative AFR, a clinical indicator based on inflammation and nutrition, plays a clear role in the predictions of patient prognosis. The prognostic prediction model incorporating pathological factors and AFR demonstrates simplicity, efficacy, and exceptional accuracy.
Trimethylamine N-oxide, S-equol, and indoxyl sulfate inflammatory microbiota players in ocular Behçet’s disease
Authors: Arslan Sermal , Kaya Mehmet Kaan , Aydin Suna , Aydin Suleyman 
Published: December 2024

Abstract

Objectives
The aims of the study were to assess the levels of serum TMAO, S-equol, and indoxyl sulfate in subjects with ocular active Behçet’s disease (OABD) and ocular inactive Behçet’s disease (OIBD).

Methods
The study involved 22 patients with OABD, 22 patients with OIBD, and thwentythree control participants. 5 mL venous blood was taken from the participants. The TMAO, S-equol, and indoxyl sulfate in the serum were measured using the ELISA method.

Results
When compared to the TMAO levels of the control group, the TMAO levels of the participants with OABD and OIBD were considerably greater (p<0.05). Similarly, when compared to the S-equol levels of the control group, the S-equol levels of the participants with OABD and OIBD were significantly higher (p<0.05). Additionally, when compared to the indoxyl sulfate of the control group, the indoxyl sulfate amounts of the participants OABD and OIBD were significantly higher (p<0.05).

Conclusions
It was first time shown that microbiota molecules could have an impact on Behçet’s disease (BD) pathogenesis. Additionally, measuring these molecules in addition to the BD Ocular Attack Score 24 (BOS24) might offer advice to medical professionals regarding the diagnosis and treatment of the illness.