bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Quest to the Therapeutic Arsenal: Novel Strategies to Combat Multidrug-
resistant Bacteria
Authors: Ashwath Priyanka , Sannejal Akhila Dharnappa 
Published: April 2021

:
The increasing resistance of the disease-causing pathogens to antimicrobial drugs is a public
health concern and a socio-economic burden. The emergence of multi-drug resistant strains has
made it harder to treat and combat infectious diseases with available conventional antibiotics. There
are currently few effective therapeutic regimens for the successful prevention of infections caused by
drug-resistant microbes. Various alternative strategies used in the recent past to decrease and limit antibiotic
resistance in pathogens include bacteriophages, vaccines, anti-biofilm peptides, and antimicrobial
peptides. However, in this review, we focus on the novel and robust molecular approach of antisense
RNA (asRNA) technology and the clustered regulatory interspaced short palindromic repeat
(CRISPR)-based antibiotic therapy, which can be exploited to selectively eradicate the drug-resistant
bacterial strain in a sequence-specific fashion establishing opportunities in the treatment of multi-drug
resistant related infections.
CRISPeering: Bioengineering the Host Cells through CRISPRCas9 Genome Editing System as the Next-generation of Cell Factories
Authors: Morowvat Mohammad H. 
Published: April 2021

Background:Nowadays, the CRISPR-Cas9 genome editing system has become a popular bioengineering-based tool for various applications. Owing to its high-target specificity, efficiency, versatility, and simplicity, it has gained attention as a robust tool for molecular biology research, which unveils the biological functions of unexplored genes and engineers the metabolic pathways. Chinese hamster ovary (CHO) cells and Escherichia coli are regarded as the most commonly used expression platforms for industrial- scale production of recombinant proteins. The emergence of the CRISPR-Cas9 genome editing system promotes the current status of expression hosts towards controllable and predictable strains.Objectives:This paper presents the current status of expression hosts for biopharmaceutical production. Some major accomplishments in the utilization of the CRISPR-Cas9 genome editing tool in the different prokaryotic and eukaryotic systems are discussed, and more importantly, the future directions of this newly arrived technology to make the next-generation cell factories with improved or novel properties are suggested. Moreover, the challenges faced in recent patents in this field are also discussed.Results and Conclusion:The CRISPR-Cas9 genome-editing tool has been adopted to be utilized in some major expression platforms. CRISPeering has been successfully employed for genome editing in different prokaryotic and eukaryotic host cells. The emergence of systems metabolic engineering, systems biology, and synthetic biology fortify the current situation of the CRISPR-Cas9 genome editing system.
Clinical, Discharge and Psychosocial Profiles of Adolescents with Substance Use Disorder Accessing Inpatient Psychiatry in Ontario, Canada
Authors: Preyde Michèle , Whitworth Katelyn , DiCroce Marco , Markov Anna , Parekh Shrenik , Heintzman John 
Published: April 2021

Objective:
The aim for this study was to explore the proportion of
patients with a Substance Use Disorder (SUD), their proportion of overall
admissions, and their psychosocial histories at a regional child and adolescent
inpatient psychiatric unit.

Methods:
A retrospective chart review was conducted on all admissions of patients
with a SUD. Abstracted patient data included demographic information, diagnoses,
psychosocial histories, living situation at the time of admission, legal involvement
and charges, whether an alternate level of care was needed, and discharge plans.

Results:
From September 2018 to February 2020, of the 540 patients admitted, 126
(23.3%) had a SUD recorded in their charts and accounted for 34.6% of the total
number of admissions. Their mean age was 15.85 (SD 1.10; range 13 to 18) years,
and most were identified as female (n=81; 64%), which is consistent with the number
of female patients admitted during the study period (n=366 female of 540 admitted;
X2=0.56, p= 0.45). Common psychiatric diagnoses included mood, trauma, and
stressor-related and anxiety disorders. Most patients (124; 98.4%) had a history of
psychosocial adversity, 90 (71.4%) patients had a parent with a mental illness or a
SUD, 55 (44%) reported being bullied, 54 (43%) reported being sexually assaulted
and several (n=41;33%) had experienced precarious living situations.

Conclusion:
Adolescent inpatients with a SUD were also managing a complex
array of clinical and psychosocial challenges. Upon discharge from the hospital,
some patients were placed on waitlists, and many patients were referred to
specialized treatment far from home, highlighting the need for additional,
comprehensive programs for SUD and the constellation of psychosocial problems
associated with these disorders.
Engaging Institutional Stakeholders to Develop and Implement Guidelines for Recruiting Participants in Research Studies Using Social Media: Mixed Methods, Multi-Phase Process
Authors: Flood-Grady Elizabeth , Solberg Lauren B , Baralt Claire , Meyer Meghan , Stevens Jeff , Krieger Janice L 
Published: April 2021

Background
Limited regulatory guidance surrounding the use of social media channels for participant recruitment is an interdisciplinary challenge. Establishing stakeholder-informed procedures is essential for ethical and effective use of social media for participant recruitment.

Objective
This study aims to provide replicable procedures for developing and implementing guidelines for using social media to recruit participants in research studies.

Methods
Social media use cases at the university were used to identify institutional stakeholders for the initiative. After establishing workflow procedures, a scoping review of web-based materials about recruitment and research on the internet and social media from 19 peer institutions and 2 federal agencies was conducted to inform the structure of the policies and procedures. End users (investigators and study coordinators; N=14) also provided feedback on the policies and procedures and implementation.

Results
Representatives (n=7) from 5 institutional offices and 15 subject-matter experts from 5 areas were identified as stakeholders in the development of policies and procedures. Peers with web-based materials (n=16) identified in the scoping review revealed 4 themes that served as a basis for developing our policies and procedures. End user feedback further informed the policies and procedures and implementation. A centrally managed social media account for communicating with participants and hosting advertising campaigns on social media was also established and, when combined with the policies and procedures, resulted in 39 advertising campaigns, and 2846 participants were enrolled in health and clinical research studies.

Conclusions
Our policies and procedures allow research teams to harness the potential of social media to increase study recruitment and participation; the transparent, stakeholder-informed process can be replicated by institutional administrators to establish policies and procedures that meet the interests and needs of their research community.
Perceptions of Mobile Health Apps and Features to Support Psychosocial Well-being Among Frontline Health Care Workers Involved in the COVID-19 Pandemic Response: Qualitative Study
Authors: Yoon Sungwon , Goh Hendra , Nadarajan Gayathri Devi , Sung Sharon , Teo Irene , Lee Jungup , Ong Marcus E H , Graves Nicholas , Teo Tess Lin 
Published: April 2021

Background
Frontline health care workers are experiencing a myriad of physical and psychosocial challenges amid the COVID-19 pandemic. There is growing recognition that digital technologies have the potential to improve the well-being of frontline workers. However, there has been limited development of wellness interventions using mobile health (mHealth) technology. More importantly, little research has been conducted on how frontline workers perceive mHealth-based support to promote their well-being.

Objective
This study aimed to explore frontline workers’ experience of conventional psychological wellness programs and their perceptions of the usefulness of mHealth apps and features for promoting well-being. It also sought to identify factors that could potentially influence uptake and retention of an mHealth-based wellness program.

Methods
We conducted semistructured interviews using purposive sampling with frontline workers involved in the COVID-19 response. Various visual materials, collated from existing mHealth app features, were presented to facilitate discussion. Interviews were audio-recorded and transcribed verbatim. Thematic analysis based on grounded theory was undertaken. Themes were subsequently mapped to key nudge strategies—those commonly used for mHealth development—to assess participants’ preferences for particular features and their reasoning.

Results
A total of 42 frontline workers participated in 12 one-on-one interviews or focus group discussions. Frontline workers generally had a limited ability to identify their own psychological problems and liked the reminders functionality of the app to track their mood over time. A personalized goal-setting feature (ie, tailoring) and in-app resources were generally valued, while frequent coaching and messages (ie, framing) were seen as a distraction. The majority of participants desired a built-in chat function with a counselor (ie, guidance) for reasons of accessibility and protection of privacy. Very few participants appreciated a gamification function. Frontline workers commonly reported the need for ongoing social support and desired access to an in-app peer support community (ie, social influence). There were, however, concerns regarding potential risks from virtual peer interactions. Intrinsic motivational factors, mHealth app technicality, and tangible rewards were identified as critical for uptake and retention.

Conclusions
Our study highlights the potential of mHealth apps with relevant features to be used as wellness tools by frontline health care workers. Future work should focus on developing a nonintrusive and personalized mHealth app with in-app counseling, peer support to improve well-being, and tangible and extrinsic rewards to foster continued use.
Kelarutan Lignin Kayu Cepat Tumbuh dalam Ekstraksi Alkali
Authors: Nawawi Deded , Fatrawana Adesna , Syafii Wasrin 
Published: April 2021

The dissolution of wood lignin in the alkali solution were analyzed for two fast growing species, sengon (Paraserianthes falcataria) dan leda (Eucalyptus deglupta). Wood samples were extracted in 1% sodium hydroxide solution in cold and hot temperatures for 24 and 1 h, respectively. Extractable substances content, permanganate consumption (expressed as assumed lignin content) were analyzed from the filtrates, lignin content and diastereomeric of β-O-4 structure were analyzed from the wood residue after alkali extraction. The extracted substances from both of wood in hot alkali was higher than in cold alkali solution, although the time of cold alkali extraction was much longer that hot alkali extraction. Assumed lignin content in the extract was quite high in both samples which were 34.4-42.9% and 35.5-39.8% including 2,15-2,29% dan 1,04-1,32% of lignin for cold and hot alkali extraction, respectively. The changes in stereo isomeric form, erythro and threo, of β-O-4 structure of lignin in wood residue indicated that partial depolimerization of lignin was takec place during alkali treatment.
Perbandingan Roundtable On Sustainable Palm Oil (RSPO), Indonesian Sustainable Palm Oil (ISPO), dan Malaysian Sustainable Palm Oil (MSPO)
Authors: Wulandari Ayu , Nasution Muhammad Ansori 
Published: April 2021

Industri minyak sawit merupakan industri yang berperan dalam sektor perekonomian suatu negara. Industri kelapa sawit membutuhkan standar sertifikasi dalam memperkuat sistem industri dan langkah untuk tetap berkelanjutan. Sistem industri yang diperkuat berdasarkan ekonomis, hukum, sosial, dan ramah lingkungan. Penerapan standar sertifikasi kelapa sawit yang berkelanjutan antara lain Roundtable on Sustainable Palm Oil (RSPO), Indonesian Sustainable Palm Oil (ISPO) dan Malaysian sustainable Palm Oil (MSPO). Penelitian ini bertujuan untuk membandingkan tiga standar sertifikasi dan mengetahui standar sertifikasi yang paling komprehensif. Metode penelitian ini menggunakan metode kualitatif yang bersifat deskriftif dan dianalisa secara komprehensif dari setiap indikator. Hasil penelitian ini menunjukkan bahwa total penilaian RSPO, ISPO dan MSPO masing-masing sebesar 77 poin, 56 poin dan 62 poin, sehingga dapat disimpulkan RSPO memiliki tingkat kedetailan dan persyaratan yang lebih komprehensif dibandingkan ISPO dan MSPO berdasarkan aspek sistem sertifikasi, sistem rantai pasok, perlindungan sosial dan lingkungan, perlakuan petani kecil/plasma, lahan gambut dan pembebasan tanah.
Vasovasostomie robot assistée : résultats sur le spermogramme post-opératoire à 3 mois & la fécondité secondaire à propos de 4 cas
Authors: Charles Thomas 
Published: April 2021

Objective: Evaluation of the efficacy of robotic vasovasostomy
post-vasectomy.
Patients and methods: We present a retrospective study of four
patients aged from 36 to 51 years, who were operated of a vasovasostomy
between September 2007 to July 2009. The same surgeon performed a
robotic-assisted vasovasostomy, bilateral for three of them and only
left unilateral for the last patient who underwent orchidectomy for
right testicular seminoma. These patients had a preoperative semen
analysis confirmed the absence of sperm after vasectomy. All patients
had an outcome of spermatozoa on testicular deferens side in
per-operative. The permeability of the distal vas deferens was
systematically checked. The success criterion was the presence of
spermatozoa in semen control three months. The paternity post
vasovasostomy without medically assisted procreation due to father
sterility was a secondary endpoint.
Results: Four patients had between 0.6 and 27 million sperm per
mL in postoperative semen analysis. Three to seventeenth months after
the vasovasostomy, the wives of four patients have started a pregnancy
between.
Conclusion: The robotic vasovasostomy surgery is a technique
that enabled this small group of patients having good results in regard
to deferential recanalization and to recovery of secondary spontaneous
fertility. A larger cohort needs to be evaluated. The medical and
economical aspects of this method should be compared to those of usual
technics, in vitro fertilization (IVF) and intracytoplasmic sperm
injection (ICSI).
Important Drop in Rate of Acute Diabetes Complications in People With Type 1 or Type 2 Diabetes After Initiation of Flash Glucose Monitoring in France: The RELIEF Study
Authors: Roussel Ronan , Riveline Jean-Pierre , Vicaut Eric , de Pouvourville Gérard , Detournay Bruno , Emery Corinne , Levrat-Guillen Fleur , Guerci Bruno 
Published: April 2021

OBJECTIVE
The RELIEF study assessed rates of hospitalization for acute diabetes complications in France before and after initiation of the FreeStyle Libre system.

RESEARCH DESIGN AND METHODS
A total of 74,011 patients with type 1 diabetes or type 2 diabetes who initiated the FreeStyle Libre system were identified from the French national claims database with use of ICD-10 codes, from hospitalizations with diabetes as a contributing diagnosis, or the prescription of insulin. Patients were subclassified based on self-monitoring of blood glucose (SMBG) strip acquisition prior to starting FreeStyle Libre. Hospitalizations for diabetic ketoacidosis (DKA), severe hypoglycemia, diabetes-related coma, and hyperglycemia were recorded for the 12 months before and after initiation.

RESULTS
Hospitalizations for acute diabetes complications fell in type 1 diabetes (−49.0%) and in type 2 diabetes (−39.4%) following FreeStyle Libre initiation. DKA fell in type 1 diabetes (−56.2%) and in type 2 diabetes (−52.1%), as did diabetes-related comas in type 1 diabetes (−39.6%) and in type 2 diabetes (−31.9%). Hospitalizations for hypoglycemia and hyperglycemia decreased in type 2 diabetes (−10.8% and −26.5%, respectively). Before initiation, hospitalizations were most marked for people noncompliant with SMBG and for those with highest acquisition of SMBG, which fell by 54.0% and 51.2%, respectively, following FreeStyle Libre initiation. Persistence with FreeStyle Libre at 12 months was at 98.1%.

CONCLUSIONS
This large retrospective study on hospitalizations for acute diabetes complications shows that a significantly lower incidence of admissions for DKA and for diabetes-related coma is associated with use of flash glucose monitoring. This study has significant implications for patient-centered diabetes care and potentially for long-term health economic outcomes.
Mapping Population Vulnerability and Community Support during COVID-19
Authors: Di Cara Nina H , Song Jiao , Maggio Valerio , Moreno-Stokoe Christopher , Tanner Alastair R , Woolf Benjamin , Davis Oliver S P , Davies Alisha 
Published: April 2021

Background Disasters such as the COVID-19 pandemic pose an overwhelming demand on resources that cannot always be met by official organisations. Limited resources and human response to crises can lead members of local communities to turn to one another to fulfil immediate needs. This spontaneous citizen-led response can be crucial to a community’s ability to cope in a crisis. It is thus essential to understand the scope of such initiatives so that support can be provided where it is most needed. Nevertheless, quickly developing situations and varying definitions can make the community response challenging to measure.
Aim To create an accessible interactive map of the citizen-led community response to need during the COVID-19 pandemic in Wales, UK that combines information gathered from multiple data providers to reflect different interpretations of need and support.
Approach We gathered data from a combination of official data providers and community-generated sources to create 14 variables representative of need and support. These variables are derived by a reproducible data pipeline that enables flexible integration of new data. The interactive tool is available online (www.covidresponsemap.wales) and can map available data at two geographic resolutions. Users choose their variables of interest, and interpretation of the map is aided by a linked bee-swarm plot.
Discussion The novel approach we developed enables people at all levels of community response to explore and analyse the distribution of need and support across Wales. While there can be limitations to the accuracy of community-generated data, we demonstrate that they can be effectively used alongside traditional data sources to maximise the understanding of community action. This adds to our overall aim to measure community response and resilience, as well as to make complex population health data accessible to a range of audiences. Future developments include the integration of other factors such as well-being.
PENINGKATAN SDM DALAM PENGEMBANGAN PEMBERDAYAAN EKONOMI MASYARAKAT MELALUI KETERAMPILAN BATIK DI NAGARI KOTO SANI KEC. X KOTO SINGKARAK KAB. SOLOK
Authors: Pebriyeni Eliya , Ahdi San , Erwin Erwin , Ernis Ernis 
Published: April 2021

AbstrakPermasalahan yang dialami oleh ibu-ibu PKK Nagari Koto Sani adalah mereka belum bisa dan belum mampu untuk mengembangkan potensi diri dan mengembangkan keterampilan. Sehingga ibu-ibu PKK belum bisa meningkatkan sumber daya manusia (SDM) guna untuk menambah penghasilan baru, atau untuk meningkatkan kebutuhan hidup sehari-hari. Maka dari itu ibu-ibu PKK Nagari Koto Sani ini membutuhkan pengetahuan, tidak hanya pengetahuan dibidang keterampilan tetapi juga bagaimana hasil yang diperoleh dari keterampilan yang diberikan tersebut dipasarkan kemasyarakat luas. Masalah prioritas yang diselesaikan dilihat dari tiga aspek kegiatan pembelajaran dan keterampilan yang saling berkorelasi, yaitu masalah pada aspek: Pemahaman dan penerapan membuat pola atau mendesain, Penguasaan dan penerapan materi keterampilan membatik, Pengetahuan tentang manajemen usaha sebagai bekal mengembangkan usaha. Metode dan pendekatan yang digunakan adalah: metode ceramah dan tanya jawab, metode demontrasi untuk menjelaskan proses pembuatan batik, metode latihan dan bimbingan dan metode resitasi atau pemberian tugas. Peserta dalam mengikuti pelatihan ini memperoleh dua jenis keterampilan, yaitu pengetahuan tentang entrepreneur/wirausaha dan mendapatkan keterampilan dalam pembuatan karya dengan menggunakan teknik batik, sekitar 80 % materi dikuasai oleh peserta. Kata Kunci: Sumber Daya Manusia, Batik, Manajemen Usaha.Kata Kunci: sumber daya manusia, batik.AbstractThe problem experienced by PKK Nagari Koto Sani mothers is that they are not yet able and unable to develop their potential and develop skills. So that PKK mothers have not been able to increase their human resources in order to add new income, or to increase their daily needs. Therefore, the women of PKK Nagari Koto Sani need knowledge, not only knowledge in the field of skills but also how the results obtained from the skills provided are marketed to the wider community. The priority problems that are resolved are seen from the three aspects of learning activities and skills that are correlated, namely problems in the following aspects: Understanding and application of making patterns or designing, Mastery and application of batik skills material, Knowledge of business management as a provision to develop a business. The methods and approaches used were: lecture and question and answer method, demonstration method to explain the batik making process, training and guidance methods and recitation or assignment methods. Participants in this training acquire two types of skills, namely knowledge of entrepreneurship / entrepreneurship and gaining skills in making works using batik techniques, about 80% of the material is controlled by participants.Keywords: human resources, batik.
Bioenergy and Bioresources Usage in the Context of Circular Economy Promotion
Authors: Melnyk Leonid , Kubatko Oleksandr , Fedyna Svitlana , Torba Ivan 
Published: April 2021

AbstractThe circular economy, which minimises the level of environmental pollution, creates a promising background for sustainable development. The application of bioeconomics principles provides the use of by-products of agriculture, forestry, food waste, and wastewater as raw materials for electricity and heat. The objective is to analyse the prerequisites for bioenergy development as one of the circular economy areas. FGLS estimations are performed over the period of 2007–2018. The leading position in terms of bioenergy sphere growth is occupied by the European Union, followed by the countries of Asia and North America. The division of biomass into “traditional” and “modern” is discussed, where traditional biomass is mostly used for cooking and space heating in developing countries, while the EU is focusing on the development of second-generation (2G) biofuels when the biofuel is produced from non-food raw materials. It is estimated that the annual production of energy from renewable sources is about 225 thousand tons of o. e. in Ukraine. It is found that the legal field and the strategic directions of the bioeconomy in the European Union and Ukraine coincide, but the pace of development of bioenergy in Ukraine can be described as very slow. To increase the bioenergy potential, it is recommended to apply green tariffs, renewable energy certificates, and subsidies to energy producers.