bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are physicians ready to comply with the guidelines for diagnosis and management of Helicobacter pylori-associated diseases: the survey results 2020–2021
Authors: Bordin D. S. , Krolevets T. S. , Livzan M. A. 
Published: November 2021

Objective: To assess compliance of physicians with diagnostic and management guidelines for H. pylori-associated diseases with a question-naire-based survey.Materials and methods: We conducted an anonymous voluntary online survey of 775 physicians of the following specialties: internal medicine 459 (59.2%), gastroenterology 279 (36%), and endoscopy 34 (4.4%). The respondents expressed their level of agreement with the questionnaire items as follows: 0 – I do not know, 1 – disagree, 2 – partially agree, and 3 – fully agree.Results: 613 (79.4%) of the physicians fully agreed to diagnose and treat H. pylori in patients with chronic atrophic gastritis, 602 (78.0%) in the 1 st degree relatives of gastric cancer patients, 525 (68.0%) in patients with chronic superficial gastritis, 423 (54.8%) in peptic ulcer at remission, and 336 (43.4%) in those with dyspepsia syndrome. The physicians were equally compliant with eradication therapy in the patients, for whom long term use of proton pump inhibitors (PPI) or non-steroid anti-inflammatory drugs (NSAID) is being planned (386 (50.0%) and 397 (51.4%), respectively). Internists were less compliant with diagnosis and management of H. pylori in patients taking both PPI (χ2 = 66.525, p = 0.004) and NSAID (χ2 = 103.354, p = 0.003). Among the primary diagnostic tools for H. pylori the physicians preferred 13/14С-urease breath test (545 physicians, or 70.6%) and gastric bioptate morphology (574, or 74.4%), and among the control diagnostic methods they chose fаeces analyses (enzyme-linked immunosorbent assay and polymerase chain reaction). The respondents considered bismuth-enhanced standard triple therapy with clarithromycin to be the most effective regimen for the 1 st line eradication therapy (606, 78.5%). To increase the efficacy of eradication therapy, the physicians were more prone to administer esomeprazole or rabeprazole (70.6%), bismuth-based agents (79.4%), than to use rebamipid (35%), probiotics (44.9%) and/or to double PPI doses (44.2%). The respondents expressed their concerns with low patient compliance to treatment (59.4%) and limited diagnostic capabilities (49.4%).Conclusion: Physician's compliance with the guidelines on diagnosis and management of H. pylori-associated diseases is adequate and might depend on both their awareness and availability of the proposed diagnostic and therapeutic methods.
Perbandingan Efektivitas Cefazolin dan Ceftriaxone Sebagai Antibiotik Profilaksis Bedah Ortopedi Open Reduction Internal Fixation (ORIF) Fraktur Tertutup
Authors: Latifah Nurul , Andayani Tri Murti , Ikawati Zullies 
Published: November 2021

<p>Infeksi daerah operasi (IDO) merupakan infeksi yang terjadi hingga 30 hari setelah operasi atau satu tahun setelah operasi pada pasien yang menerima implan dan mempengaruhi sayatan atau jaringan dalam di lokasi operasi. Sefalosporin golongan pertama yaitu cefazolin merupakan antibiotik pilihan sebagai proflaksis bedah karena merupakan antibiotik spektrum luas, memiliki profil farmakokinetik yang baik, angka kejadian efek samping yang kecil, serta harga relatif murah. Pada praktek klinis, ceftriaxone banyak digunakan karena merupakan antibiotik yang lebih murah dibanding cefazolin. Tujuan penelitian ini adalah untuk mengetahui efektivitas dan keamanan cefazolin dibandingkan dengan ceftriaxone sebagai antibiotik profilaksis pasien bedah ortopedi <em>Open Reduction Internal Fixation </em>(ORIF) fraktur tertutup. Penelitian ini merupakan penelitian observasional analitik dengan rancangan kohort retrospektif dari rekam medis pasien yang menjalani ORIF fraktur tertutup periode 1 Januari hingga 31 Desember 2020 di sebuah rumah sakit swasta tipe B di Yogyakarta. Luaran klinis yang diamati dalam penelitian ini adalah kejadian IDO dan kejadian efek samping selama 90 hari sejak pasien dilakukan prosedur bedah<em>. </em>Terdapat 59 pasien yang menjalani ORIF yang masuk kriteria inklusi pada penelitian ini yang terbagi mejadi dua kelompok yaitu pasien yang mendapat antibiotik profilaksis cefazolin (n=25) dan ceftriaxone (n=34). Hasil dari penelitian ini menunjukkan bahwa angka kejadian IDO pada pasien yang menjalani ORIF fraktur tertutup sebesar 5,08% dari total subjek atau 8,82% dari yang diberikan ceftriaxone dan 0% dari pasien yang diberikan cefazolin. Perbedaan probabilitas kejadian IDO pada pemberian ceftriaxone sebagai antibiotik profilaksis tidak berbeda bermakna dengan pemberian cefazolin (RR = 1,097; 95% CI = 0,988 – 1,218; p = 0,064).</p>
STK11 pathway in vitro validation
Authors: Lehtiö Janne , Arslan Taner , Siavelis Ioannis , Pan Yanbo , Socciarelli Fabio , Berkovska Olena , Umer Husen M. , Mermelekas Georgios , Pirmoradian Mohammad , Jönsson Mats , Brunnström Hans , Brustugun Odd Terje , Purohit Krishna Pinganksha , Cunningham Richard , Asl Hassan Foroughi , Isaksson Sofi , Arbajian Elsa , Aine Mattias , Karlsson Anna , Kotevska Marija , Hanson Carsten Gram , Haakensen Vilde Drageset , Helland Åslaug , Tamborero David , Johansson Henrik J. , Branca Rui M. , Staaf Johan , Orre Lukas M. 
Published: November 2021

Abstract
The associated publication reports proteogenomic analysis of non-small cell lung cancer (NSCLC), where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, and validated our classification method in separate clinical cohorts. The in-depth proteomic analysis pointed to a potential role of STK11 inactivation in liver-specific signaling and subsequent cancer growth and immune evasion mechanisms. This protocol describes in vitro validation of the downstream effects of STK11-AMPK signaling on HNF1A and FGL1 in a liver and two lung cancer cell lines.
Development of a Serial Dilution Technique for Obtaining Monoclonal Cell Populations
Authors: Ranjbar Maryam , Nourigorji Marjan , Amiri Farshid , Khamirani Hossein Jafari , Zoghi Sina , Dianatpour Mehdi 
Published: November 2021

Abstract
Single cell-based techniques have drawn the attention of researchers, because they provide invaluable information of various domains ranging from genomics to epigenetics, transcriptomics, and proteomics. Single cell-derived clones provide a reliable and sustainable source of genetic information due to the homogeneity of the cell population. Aiming to obtain single-cell clones, several approaches were engineered, among which, the Limiting dilution approach stands out as a cost-effective and unsophisticated, and easy-to-apply method. Here, we demonstrate how to acquire single cell-derived clones through a simple 1:10 diluting from genetically modified heterogeneous cell populations.
Learning Neuroplastic Matching of Robot Dynamics in Closed-loop
CPGs
Authors: Ruppert Felix , Badri-Spröwitz Alexander 
Published: November 2021

Abstract
Legged robots have the potential to show locomotion performance with reduced control effort and energy efficiency by leveraging elastic structures inspired by animals' elastic tendons and muscles.
However, it remains a challenge to match the natural dynamics of complex legged robots and their control task dynamics.
Here we present a framework to match control task dynamics and natural dynamics based on the neuroelasticity and neuroplasticity concept.
Inspired by animals we design quadruped robot Morti with strong natural dynamics as a testing platform.
It is controlled through a bioinspired closed-loop central pattern generator (CPG) that is designed to neuroelastically mitigate short term perturbations using sparse contact feedback.
We use the amount of neuroelastic activity as a proxy to quantify the dynamics' mismatching.
By minimizing neuroelastic activity, we neuroplastically match the control task dynamics to the robot's natural dynamics.
Through matching the robot learns to walk within one hour with only sparse feedback and improves its energy efficiency without explicitly minimizing it in the cost function.
Association of Serum β-Hydroxybutyrate and Coronary Artery Disease in an Urban Chinese Population
Authors: Mu Hongna , Yang Ruiyue , Wang Siming , Zhang Wenduo , Wang Xinyue , Li Hongxia , Dong Jun , Chen Wenxiang , Yu Xue , Ji Fusui 
Published: November 2021

Abstract
Background: Ketone bodies, including β-hydroxybutyrate, acetoacetate, and acetone, can substitute and alternate with glucose under conditions of fuel and food deficiency. Ketone body metabolism is increased in a myriad of tissue metabolism disorders. Perturbations in metabolism are major contributors to coronary artery disease (CAD). The present study aimed to investigate the association of β-hydroxybutyrate with CAD. Methods: A total of 2970 Chinese Han population were enrolled. Gensini scores were calculated for all patients with positive findings. Serum levels of β-hydroxybutyrate and other laboratory parameters were measured. Associations of serum metabolites with traditionary risk factors and CAD severity were analyzed. Results: Our results showed that β-hydroxybutyrate was associated with some CAD risk factors and CAD severity, determined by Gensini score or number of diseased regions. Moreover, β-hydroxybutyrate was associated with T3/T1 of Gensini score tertile after adjusting for traditional risk factors by multivariable logistic regression analysis. The association of β-hydroxybutyrate with CAD severity was more obvious in female. Conclusions: Taken together, circulating β-hydroxybutyrate level was independently associated with CAD severity, and the association was more pronounced in female.
Overexpression of The AtWUSCHEL Gene Promotes Somatic Embryogenesis And Lateral Branch Formation In Birch (Betula Platyphylla Suk.)
Authors: Lou Hu , Wang Weizhi , Yang Linlin , Cai Zhiyong , Cai Huiying , Liu Zhiqi , Sun Long , Xu Qijiang 
Published: November 2021

Abstract
Birch (Betula platyphylla Suk.) is a deciduous tree with the value of medicinal and ornamental greening. Plant somatic embryogenesis is a limiting step in birch genetic breeding. As a transcription factor, the Arabidopsis thaliana WUSCHEL (AtWUS) gene plays an important role in maintaining and regulating stem cell characteristics. It determines whether the stem cell population is differentiated. To explore the method of inducing somatic embryogenesis in birch. We overexpressed the AtWUS gene and transferred it into birch. The expression of AtWUS increased the somatic embryogenesis rate from 101.4% to 717.1%. The expression of the AtWUS gene in calli and globular embryos led to the downregulation of the BpWUS gene. The BpLEC1, BpLEC2, BpFUS3 and BpABI3 genes were upregulated. In addition, overexpression of AtWUS increased the number of lateral branches and bud meristem in birch. Similarly, the BpWUS gene was downregulated in the bud meristem. The BpLEC1, BpLEC2, BpFUS3, BpSTM and BpCUC2 genes were upregulated. This result indicated that overexpression of the AtWUS gene promoted somatic embryogenesis (SE) by increasing the expression of SE-related genes. In conclusion, this study focused on the role of the AtWUS gene in birch SE and the molecular mechanism of promoting SE.
How COVID-19 Infection can Alter the Sex Hormones in Hospitalized Patients? A Longitudinal Study in Iran
Authors: Afshari Poorandokht , Zakerkish Mehrnoosh , Abedi Parvin , Beheshtinasab Maryam , Maraghi Elham , Meghdadi Hossein 
Published: November 2021

Abstract
Background: There is some evidence about alteration of sex hormones in patients with COVID-19 infection. This study aimed to evaluate the levels of sex hormones in female and male patients with COVID-19 during hospitalization and one month after discharge. Methods: The levels of sex hormones including estradiol, progesterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), total testosterone, and free testosterone were measured in 162 female and male patients with COVID-19 infection during hospitalization and one month after discharge. A demographic questionnaire and a checklist were used to collect the data. The ANCOVA test was used to compare the level of hormones in patients with severe and moderate disease. Results: In the primary assessment, 162 patients were assessed for serum levels of sex hormones, while a month after discharge, only 69 patients provided consent for assessment, and nine had passed away. During hospitalization, female patients with severe disease had an elevated level of estradiol (407.70±623.37 pg/mL) in comparison to those with a moderate disease (213.78±407.17 pg/mL). The levels of progesterone and LH were high during hospitalization, but there was a decrease in these levels after discharge. The reduction in the level of FSH in patients with severe disease was greater than in patients with moderate disease, which increased after discharge. While the level of testosterone decreased during hospitalization, the alteration of free testosterone was negligible in male patients.Conclusion: In this study, we observed alteration in sex hormones (increased level of estrogen, progesterone, LH and reduction in the level of FSH and total testosterone) in female and male patients, with the alteration being greater in the latter. Due to the attrition of patients in follow-up period, more studies are needed to confirm these results.
The Trajectory and Influence Factors of Breast Cancer Patients’ Main Chemotherapy-Related Symptoms: A Longitudinal Study
Authors: Qi Yishu , Zhang Ning , Ma Ye , Xu Ewen , Huang Qingmei , Lin Lu , bao zhaokang , Qi Jie , Chen Jianhua , Chen Xiaokang , Tian Li 
Published: November 2021

Abstract
Introduction: Identifying the pattern of change in symptoms is critical to effective symptom management. This study aimed to determine the trajectory of Main Chemotherapy-related Symptoms (MCRS) in breast cancer patients, explore the influencing factors of potential categories of MCRS trajectory.Methods: Patient-reported Outcomes Measurement System- breast-chemotherapy was used to measure the four highest incidence MCRS (pain, fatigue, anxiety, and depression) weekly in Breast cancer patients. The Growth Mixture Model (GMM) was used to fit the potential categories of the MCRS trajectory. Logistic regression was used to explore the influencing factors of potential categories of MCRS change trajectory.Results: 239 breast cancer patients completed the study. Fatigue and depression showed an overall upward trend during the chemotherapy cycle, while pain and anxiety showed a downward trend. There are two potential categories of anxiety trajectory, three potential categories of fatigue and pain trajectory, and four potential categories of depression trajectory. Compared with the mild-fatigue group, Patients in the moderate and high fatigue groups were more likely to be less educated, have lower household income, and be treated with anthracyclines. Compared with the mild-pain group, patients in the pain-declining and fluctuating-pain groups were young, live-alone, and treated with paclitaxel. Patients in the anxiety-rising group were younger, had premenopausal menstruation with regular monthly menstruation, and had stage II disease. Patients in the depression-rising and severe depression groups were more likely to be solitary and younger.Conclusion: The potential classes of major chemotherapy-related symptom trajectories vary in breast cancer patients. As for fatigue management, great attention should be paid to patients with low education, low family income, and anthracycline chemotherapy. For pain management, close attention should be paid to younger, solitary, and paclitaxel chemotherapy patients; For anxiety management, attention should be paid to younger patients with premenopausal menstruation and regular monthly menstruation patients, and those with stage II disease. In managing depression, attention should be paid to younger and solitary patients.
Unveiling The Potential Tolerance And Physiological Responses Mechanisms of Wheat (Ningmai 16) After Exposure to Nickel In a Soil-plant System
Authors: Wang Gehui , Tariq Muhammad , Liu Fang , Xiao Qinran , Peng Cheng , Zhang Wei , Yang Jie 
Published: November 2021

Abstract
Nickel (Ni) accumulation in soils could lead to critical risks to plants, animals and humans. This study aims to unveil the adverse impacts of Ni on wheat Ningmai 16 and toxicity tolerance mechanisms. Lipid peroxidation was significantly facilitated under high Ni stress, mainly reflecting the notable accumulation of malondialdehyde followed by the increase in glutathione-S-transferase (GST) activities. High Ni contamination caused the decrease in chlorophyll content, with the remarkable inhibition of root activity. Transmission electron microscopy (TEM) micrographs further confirmed the toxicity of Ni and provided significant supporting evidence for the accumulation and localisation of Ni in wheat. The mechanisms adopted in alleviating the oxidative damages induced by Ni were revealed by increasing the soluble sugar and proline content, and inducing the GST related gene expression. Additionally, the cell-wall thickening and vacuole compartmentation were also produced in wheat for improving the tolerance of Ni. Finally, most of biochemical parameters indicated a clearly positive or negative relationship with the available Ni contents, and they are proved as good biomarkers to predict the bioavailability of Ni in a soil-wheat system. We believe that our concluding findings would open a new window for a deeper understanding of ecological risks of an often-underestimated element Ni in future endeavors.
A Patient-Focused Questionnaire Designed to Support Headache Diagnosis in General Practice
Authors: Ruttledge Martin , Broessner Gregor , Gendolla Astrid , Hollanders Els 
Published: November 2021

Abstract
Background: There is an unmet need for the correct diagnosis of primary headache disorders, such as migraine, in primary care. Misdiagnosis is associated with suboptimal management of patients, and it is now widely accepted internationally that better diagnostic support is needed for general practitioners (GPs). In this study, we describe the development of a short, patient-directed questionnaire and supporting documents that aim to help with the diagnosis of headache disorders in primary care. We have also prepared patient feedback material and collected preliminary input from patients, but the main aim of this report is to invite comment and debate on the use of the questionnaire in real-life clinical practice.Methods: This questionnaire was developed over 18 months using the clinical experience of the authors, current literature review and the International Classification of Headache Disorders (ICHD), 3rd edition, for migraine, tension-type headache, cluster headache and medication-overuse headache. The questionnaire and two supporting documents will hopefully assist the GP to make a correct diagnosis. A patient survey was used to gather feedback from a small number of patients, and based on these comments, the questionnaire and the supporting documents were modified and updated.Results: Feedback gathering was attempted in Austria, Germany, Switzerland, Ireland and Spain, but was only possible in Austria due to the COVID-19 pandemic restrictions. From the 18 patients who participated, 17 responded about how easy or difficult the questionnaire was to complete, with 14/17 (82%) being able to complete the questionnaire easily on their own. Overall, the patients found the questionnaire averagely helpful in reminding them of and communicating their headache triggers, symptoms and behaviour changes; on a scale of 1–5, with 1 being very helpful and 5 being not helpful at all, the mean scores were 2.8 and 2.7 (n=18), respectively. Conclusions: This questionnaire and associated documents were developed with a view to helping GPs to make an accurate headache diagnosis quickly in primary care. Following feedback from patients, updates have been made, including changes to reduce the time it takes to complete the questionnaire. Next steps include wider validation and feedback from primary care physicians.
Partial Relay Selection for Secure Cooperative NOMA Networks
Authors: Alouane Wided Hadj 
Published: November 2021

Abstract
In this paper, we investigate physical layer security of multi-relay non-orthogonal multiple access (NOMA) networks with partial relay selection considering decode-and-forward (DF) and amplify-and-forward (AF) protocols. We propose a partial relay scheme aiming to select the best relay based on the highest signal-to-noise-ratio (SNR) of the ﬁrst link. We derive new exact and asymptotic expressions for strictly positive secrecy capacity (SPSC) and secrecy outage probability (SOP) considering Rayleigh fading channels. Numerical results demonstrate that AF and DF provide almost a similar secrecy performance. Moreover, they prove that partial relay selection improves SPSC and reduces SOP when the relay-cluster is closer to the legitimate receiver.
How Can We Develop a Standard Interface That Easily Integrates With HL7, Boosting EHR Interoperability?
Authors: BISWAS PROSENJIT 
Published: November 2021

Abstract
Background: It appears that a systematic strategy that includes the basic underpinnings of health care, from systems and technologies to clinical understanding and workflow processes, is required to achieve the next great leap in improving quality and safety. To make meaningful progress, health-care systems integration is essential. However, to provide interoperability in EHR, systems must support all versions of HL7.Software companies and healthcare organisations are still having trouble integrating HL7 with multiple interfaces. Thus, a standard interface or platform is required that easily integrates with HL7 boosting EHR interoperability. Methods: Basic Modelling Interface (BMI) interoperability standards will be the focus of this research. The research plan proposes a qualitative research approach that includes clinicians’ interview and a panel discussion to characterise the information flow models. Qualitative research using a thematic analysis of semi structured interviews will be conducted in the proposed research plan. Results: Gaining the HL7 standard is insufficient for integrating EHR with diverse apps or software in use, thus clinicians' willingness to adopt a common interface is critical. Medical practitioners and software vendors agreement required to develop an API that tackles HL7 integration issues and EHR interoperability. Planning techniques for dealing with HL7 issues and doing coordinated testing can help identify and install HL7 standard interfaces successfully. Conclusions: The proposed research plan will help us to create a standard interface for HL7 integration which will benefit clinicians, healthcare professionals, and groups such as patients, healthcare authorities, and EHR vendors. Lastly, reducing or eliminating healthcare concerns will help the economy, ecosystem, and society in both concrete and qualitative ways.