bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of river water–groundwater–seawater interactions in the coastal delta of Bangladesh based on hydrochemistry and salinity distribution
Authors: Roy Sushanta Kumar , Zahid Anwar 
Published: March 2021

AbstractA synchronization study among hydrochemistry, hydrochemical facies evaluation, EC observation, salinity distribution and groundwater flow direction has been addressed to assess river water–groundwater–seawater interactions in the coastal delta of southern Bangladesh. The findings show that river water, shallow groundwater and deep groundwater interact with seawater at various intensities within the complex dynamics of hydrochemical facies evaluation. Deep groundwater is intensively influenced by seawater, where shallow groundwater is moderately affected and river water is very negligibly affected. Major cation and anion have been plotted in the Piper diagrams and hydrochemical facies diagrams (HFE-D) to clarify the result. More than 60% of the water samples of the river lie on the Ca-HCO3 (or Mg-HCO3) facies quadrant, and more than 70% of the shallow groundwater samples and more than 95% of the deep groundwater samples lie on the Na-Cl facies quadrant of the HFE-D diagram. River water types are dissimilar, and approximately 82% of facies are characterized by freshening phases and 18% by intrusion phases. Mixed water types with predominate of Na-Cl were observed in shallow groundwater where the hydrochemical facies are characterized by 53 percent freshening phases and 47 percent intrusion phases. Deep groundwater hydrochemistry clearly indicates the dominant Na-Cl type of water in the study area where only four hydrochemical facies are observed and 78 percent correspond to the intrusion phases and 22 percent to the freshening phases. Both direct and reverse cation exchange reactions take place in shallow groundwater, where deep groundwater is predominantly characterized by reverse cation exchange reactions. Two end members: seawater of Bay of Bengal and freshwater, contribute to the exchange reactions in the coastal aquifer of the study area. In terms of nitrate contamination, river waters are affected by negligible to low concentrations, shallow groundwater is affected by moderate to high concentrations and deep groundwater is affected by moderate to very high nitrate concentrations. Dissimilarity in electrical conductivity (EC) values, variation of salinity distribution maps and groundwater flow direction suggest the possible interconnections among river water, groundwater and aquifer sediments. Significant concentrations of Na+ and Cl− ions lead to seawater contamination in groundwater, and HCO3− along with Na+, Ca2+, Mg2+ in river water suggests mixing of freshwater and seawater, which could have adverse effects both in coastal delta aquatic life and in agriculture.
Targeting unfolded protein response: a new horizon for disease control
Authors: Khanna Madhu , Agrawal Nishtha , Chandra Ramesh , Dhawan Gagan 
Published: March 2021

AbstractUnfolded protein response (UPR) is an evolutionarily conserved pathway triggered during perturbation of endoplasmic reticulum (ER) homeostasis in response to the accumulation of unfolded/misfolded proteins under various stress conditions like viral infection, diseased states etc. It is an adaptive signalling cascade with the main purpose of relieving the stress from the ER, which may otherwise lead to the initiation of cell death via apoptosis. ER stress if prolonged, contribute to the aetiology of various diseases like cancer, type II diabetes, neurodegenerative diseases, viral infections etc. Understanding the role of UPR in disease progression will help design pharmacological drugs targeting the sensors of signalling cascade acting as potential therapeutic agents against various diseases. The current review aims at highlighting the relevance of different pathways of UPR in disease progression and control, including the available pharmaceutical interventions responsible for ameliorating diseased state via modulating UPR pathways.
Total disconnectedness of Julia sets of random quadratic polynomials
Authors: LECH KRZYSZTOF , ZDUNIK ANNA 
Published: March 2021

AbstractFor a sequence of complex parameters
$(c_n)$
we consider the composition of functions
$f_{c_n} (z) = z^2 + c_n$
, the non-autonomous version of the classical quadratic dynamical system. The definitions of Julia and Fatou sets are naturally generalized to this setting. We answer a question posed by Brück, Büger and Reitz, whether the Julia set for such a sequence is almost always totally disconnected, if the values
$c_n$
are chosen randomly from a large disc. Our proof is easily generalized to answer a lot of other related questions regarding typical connectivity of the random Julia set. In fact we prove the statement for a much larger family of sets than just discs; in particular if one picks
$c_n$
randomly from the main cardioid of the Mandelbrot set, then the Julia set is still almost always totally disconnected.
Active ageing policies and delaying retirement: comparing work-retirement transitions in Austria and Germany
Authors: Schmidthuber Lisa , Fechter Charlotte , Schröder Heike , Hess Moritz 
Published: March 2021

AbstractWe investigate how labour market and pension measures associated with active ageing influence retirement behaviour in Austria and Germany. We focus on two conservative welfare states and evaluate how individuals respond to comparable pension scheme changes. Using the Survey of Health, Ageing and Retirement in Europe, findings point to increasing average actual retirement ages in both countries. Early retirement becomes less important while working until pension age has gained in significance. In particular, findings point towards greater de-standardisation of retirement transitions, though to a different extent across the two countries. Whereas gender differences are still prevalent in Austria, in line with traditional conservative welfare state characteristics, we find that Germany exhibits lower gender differences, but instead displays stronger inequalities between education groups. We argue that social risks emerge in Germany that are usually found in liberal welfare states. We suggest that this trend is reinforced by retirement policies that focus on “pushing” individuals out of employment. This study contributes to the understanding of how individuals respond to national policy incentives when making retirement transitions.
Moral navigation and child fostering in Chiawa, Zambia
Authors: White Sarah C. , Jha Shreya 
Published: March 2021

AbstractThis article explores the movement of children between households in Zambia as a site of ‘moral navigation’. Moral navigation extends Henrik Vigh's concept of social navigation from contexts of conflict and migration to more socially stable contexts in which well-being depends critically on people's ability to manage relationships. The live, dynamic and mobile character of these relationships means that they require active, real-time cultivation and response. While having practical objectives, these negotiations are also moral, articulated with ideas of what ought to be, and seeking to fulfil sometimes competing ethical projects. Life history interviews present three main perspectives: recollections of times in childhood spent away from birth parents; birth parents’ reflections on having a child living with others; and adults’ accounts of taking in other people's children. Strong norms of kinship unity and solidarity notwithstanding, in practice terms of engagement are differentiated through gender, marital, social and economic status, plus relational and geographical proximity. The pursuit of personal benefit contains the seeds of both contradiction and convergence with the collective good, as a relational understanding of moral selves sees one's own gain as proper, rightful and virtuous when it is realized in and through providing for others.
Trois idées directrices de la philosophie de Dilthey
Authors: Rioux Jean-François 
Published: March 2021

RÉSUMÉDans cet article, je propose une interprétation de la philosophie de Wilhelm Dilthey qui repose sur trois idées directrices. La première idée identifie l'objet de sa philosophie : la vie. La deuxième idée établit sa méthode, soit l'autoréflexion. La troisième idée clarifie ce qui est révélé par l'autoréflexion, à savoir l'universalité et la variabilité de la vie. Dans un premier temps, je défends que la lecture épistémologique de la philosophie de Dilthey doit être abandonnée. Dans un deuxième temps, je présente mon interprétation de la philosophie de Dilthey.
Everolimus treatment in a 3-month-old infant with tuberous sclerosis complex cardiac rhabdomyoma, severe left ventricular outflow tract obstruction, and hearing loss
Authors: Çetin Mecnun , Aydın Aysun A. , Karaman Kamuran 
Published: March 2021

AbstractTuberous sclerosis complex is a rare multisystem genetic disorder characterised by the growth of numerous tumour-like malformations in many parts of the body including skin, kidneys, brain, lung, eyes, liver, and heart. Mutations in the TSC1 or TSC2 genes have been reported to cause disruption in the TSC1–TSC2 intracellular protein complex, causing over-activation of the mammalian target of rapamycin protein complex. In this study, we present a 3-month-old male infant diagnosed with tuberous sclerosis, bilateral neurosensorial hearing loss, Wolff–Parkinson–White syndrome on electrocardiography, multiple cardiac rhabdomyomas with severe stenosis in the left ventricular outflow tract, who responded well to the Everolimus therapy.
Characterizing Black Carbon and Gaseous Pollutants on the Yangtze River Across Eastern China Continent
Authors: Ting Y. , Liu D. , Morgan W. T. , Allan J. D. , Coe H. , Li C. , Wang X. , Ye X. , Chen J. 
Published: March 2021

AbstractThe Yangtze River (YR) region and cities along the river have severe deterioration of air quality due to urbanization and economic development. Black carbon (BC) has significant impacts on air quality and climate, however it is poorly characterized across the region. Here we report the first continuous measurements of BC and gaseous pollutants along the YR in wintertime, with an intensive observation period from November 21 to December 4, 2015, covering ∼7° longitude over the continent in China. The highly polluted periods (HPP) with NOx >80 μg m−3 indicated more localized emissions. At the western (longitude <118°E) and eastern (longitude >118°E) ends of the YR valley, the HPP events reduced, mostly since the western end was influenced by terrain height and regional transport, and the eastern end was influenced by easterly oceanic or westerly continental air masses. In these regions, BC core size decreased by 5%–19% during the HPP, and had modes of mass median diameter in the range 120 –180 nm, which was found to be predominately from local sources, contrasting with a more consistent mode ∼200 nm that was due to BC from mixed sources. A comparison of simultaneously measured data from the nearby monitoring stations along the river demonstrated a high consistency of PM2.5 over the river, which reflected the important regional impact on the particulate mass. This study evaluates the shipping emission and regional influence over the YR and may guide the policy maker on mitigating air pollution.
Causes and consequences of pattern diversification in a spatially self-organizing microbial community
Authors: Goldschmidt Felix , Caduff Lea , Johnson David R 
Published: March 2021

Abstract
Surface-attached microbial communities constitute a vast amount of life on our planet. They contribute to all major biogeochemical cycles, provide essential services to our society and environment, and have important effects on human health and disease. They typically consist of different interacting genotypes that arrange themselves non-randomly across space (referred to hereafter as spatial self-organization). While spatial self-organization is important for the functioning, ecology, and evolution of these communities, the underlying determinants of spatial self-organization remain unclear. Here, we performed a combination of experiments, statistical modeling, and mathematical simulations with a synthetic cross-feeding microbial community consisting of two isogenic strains. We found that two different patterns of spatial self-organization emerged at the same length and time scales, thus demonstrating pattern diversification. This pattern diversification was not caused by initial environmental heterogeneity or by genetic heterogeneity within populations. Instead, it was caused by nongenetic heterogeneity within populations, and we provide evidence that the source of this nongenetic heterogeneity is local differences in the initial spatial positionings of individuals. We further demonstrate that the different patterns exhibit different community-level properties; namely, they have different expansion speeds. Together, our results demonstrate that pattern diversification can emerge in the absence of initial environmental heterogeneity or genetic heterogeneity within populations and can affect community-level properties, thus providing novel insights into the causes and consequences of microbial spatial self-organization.
Hepatic HuR protects against the pathogenesis of non-alcoholic fatty liver disease by targeting PTEN
Authors: Tian Mi , Wang Jingjing , Liu Shangming , Li Xinyun , Li Jingyuan , Yang Jianmin , Zhang Cheng , Zhang Wencheng 
Published: March 2021

AbstractThe liver plays an important role in lipid and glucose metabolism. Here, we show the role of human antigen R (HuR), an RNA regulator protein, in hepatocyte steatosis and glucose metabolism. We investigated the level of HuR in the liver of mice fed a normal chow diet (NCD) and a high-fat diet (HFD). HuR was downregulated in the livers of HFD-fed mice. Liver-specific HuR knockout (HuRLKO) mice showed exacerbated HFD-induced hepatic steatosis along with enhanced glucose tolerance as compared with control mice. Mechanistically, HuR could bind to the adenylate uridylate-rich elements of phosphatase and tensin homolog deleted on the chromosome 10 (PTEN) mRNA 3′ untranslated region, resulting in the increased stability of Pten mRNA; genetic knockdown of HuR decreased the expression of PTEN. Finally, lentiviral overexpression of PTEN alleviated the development of hepatic steatosis in HuRLKO mice in vivo. Overall, HuR regulates lipid and glucose metabolism by targeting PTEN.
E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability
Authors: Xu Huimin , Ju Lingao , Xiong Yaoyi , Yu Mengxue , Zhou Fenfang , Qian Kaiyu , Wang Gang , Xiao Yu , Wang Xinghuan 
Published: March 2021

AbstractE3 ubiquitin ligase RNF126 (ring finger protein 126) is highly expressed in various cancers and strongly associated with tumorigenesis. However, its specific function in bladder cancer (BCa) is still debatable. Here, we found that RNF126 was significantly upregulated in BCa tissue by TCGA database, and our studies indicated that downregulation of RNF126 significantly inhibited cell proliferation and metastasis through the EGFR/PI3K/AKT signaling pathway in BCa cells. Furthermore, we identified PTEN, an inhibitor of the PI3K/AKT signaling pathway, as a novel substrate for RNF126. By co-immunoprecipitation assays, we proved that RNF126 directly interacts with PTEN. Predominantly, PTEN binds to the C-terminal containing the RING domain of RNF126. The in vivo ubiquitination assay showed that RNF126 specifically regulates PTEN stability through poly-ubiquitination. Furthermore, PTEN knockdown restored cell proliferation, metastasis, and tumor formation of BCa cells inhibited by RNF126 silencing in vitro and in vivo. In conclusion, these results identified RNF126 as an oncogene that functions through ubiquitination and degradation of PTEN in BCa.
Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC
Authors: Zhang Guolong , Yan Guorong , Fu Zhiliang , wu Yuhao , Wu Fei , Zheng Zhe , Fang Shan , Gao Ying , Bao Xunxia , Liu Yeqiang , Wang Xiuli , Zhu Sibo 
Published: March 2021

AbstractCutaneous squamous cell carcinoma (cSCC) is prevalent in the world, accounting for a huge part of non-melanoma skin cancer. Most cSCCs are associated with a distinct pre-cancerous lesion, the actinic keratosis (AK). However, the progression trajectory from normal skin to AK and cSCC has not been fully demonstrated yet. To identify genes involved in this progression trajectory and possible therapeutic targets for cSCC, here we constructed a UV-induced cSCC mouse model covering the progression from normal skin to AK to cSCC, which mimicked the solar UV radiation perfectly using the solar-like ratio of UVA and UVB, firstly. Then, transcriptome analysis and a series of bioinformatics analyses and cell experiments proved that Rorα is a key transcript factor during cSCC progression. Rorα could downregulate the expressions of S100a9 and Sprr2f in cSCC cells, which can inhibit the proliferation and migration in cSCC cells, but not the normal keratinocyte. Finally, further animal experiments confirmed the inhibitory effect of cSCC growth by Rorα in vivo. Our findings showed that Rorα would serve as a potential novel target for cSCC, which will facilitate the treatment of cSCC in the future.
RETRACTED ARTICLE: MYC-mediated upregulation of PNO1 promotes
glioma tumorigenesis by activating THBS1/FAK/Akt signaling
Authors: Chen Xu , Guo Zheng-Qian , Cao Dan , Chen Yong , Chen Jian 
Published: March 2021

AbstractPNO1 has been reported to be involved in tumorigenesis, however, its
role in glioma remains unexplored. In the present study, PNO1 expression in glioma
from on-line databases, cDNA, and tissue microarrays was upregulated and associated
with poor prognosis. PNO1 knockdown inhibits tumor cell growth and invasion both in
vitro and in vivo; whereas PNO1 overexpression promoted cell proliferation and
invasion in vitro. Notably, PNO1 interacted with THBS1 and the promotion of glioma
by PNO1 overexpression could be attenuated or even reversed by simultaneously
silencing THBS1. Functionally, PNO1 was involved in activation of FAK/Akt pathway.
Moreover, overexpressing MYC increased PNO1 promoter activity. MYC knockdown
decreased PNO1 and THBS1 expression, while inhibited cell proliferation and
invasion. In conclusion, MYC-mediated upregulation of PNO1 contributes to glioma
progression by activating THBS1/FAK/Akt signaling. PNO1 was reported to be a tumor
promotor in the development and progression of glioma and may act as a candidate of
therapeutic target in glioma treatment.
Genome-wide haplotype association study in imaging genetics using whole-brain sulcal openings of 16,304 UK Biobank subjects
Authors: Karkar Slim , Dandine-Roulland Claire , Mangin Jean-François , Le Guen Yann , Philippe Cathy , Deleuze Jean-François , Pierre-Jean Morgane , Le Floch Edith , Frouin Vincent 
Published: March 2021

AbstractNeuroimaging-genetics cohorts gather two types of data: brain imaging and genetic data. They allow the discovery of associations between genetic variants and brain imaging features. They are invaluable resources to study the influence of genetics and environment in the brain features variance observed in normal and pathological populations. This study presents a genome-wide haplotype analysis for 123 brain sulcus opening value (a measure of sulcal width) across the whole brain that include 16,304 subjects from UK Biobank. Using genetic maps, we defined 119,548 blocks of low recombination rate distributed along the 22 autosomal chromosomes and analyzed 1,051,316 haplotypes. To test associations between haplotypes and complex traits, we designed three statistical approaches. Two of them use a model that includes all the haplotypes for a single block, while the last approach considers each haplotype independently. All the statistics produced were assessed as rigorously as possible. Thanks to the rich imaging dataset at hand, we used resampling techniques to assess False Positive Rate for each statistical approach in a genome-wide and brain-wide context. The results on real data show that genome-wide haplotype analyses are more sensitive than single-SNP approach and account for local complex Linkage Disequilibrium (LD) structure, which makes genome-wide haplotype analysis an interesting and statistically sound alternative to the single-SNP counterpart.
The limits of normal approximation for adult height
Authors: Slavskii Sergei A. , Kuznetsov Ivan A. , Shashkova Tatiana I. , Bazykin Georgii A. , Axenovich Tatiana I. , Kondrashov Fyodor A. , Aulchenko Yurii S. 
Published: March 2021

AbstractAdult height inspired the first biometrical and quantitative genetic studies and is a test-case trait for understanding heritability. The studies of height led to formulation of the classical polygenic model, that has a profound influence on the way we view and analyse complex traits. An essential part of the classical model is an assumption of additivity of effects and normality of the distribution of the residuals. However, it may be expected that the normal approximation will become insufficient in bigger studies. Here, we demonstrate that when the height of hundreds of thousands of individuals is analysed, the model complexity needs to be increased to include non-additive interactions between sex, environment and genes. Alternatively, the use of log-normal approximation allowed us to still use the additive effects model. These findings are important for future genetic and methodologic studies that make use of adult height as an exemplar trait.
A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors
Authors: Townshend Brent , Xiang Joy S. , Manzanarez Gabriel , Hayden Eric J. , Smolke Christina D. 
Published: March 2021

AbstractBiosensors are key components in engineered biological systems, providing a means of measuring and acting upon the large biochemical space in living cells. However, generating small molecule sensing elements and integrating them into in vivo biosensors have been challenging. Here, using aptamer-coupled ribozyme libraries and a ribozyme regeneration method, de novo rapid in vitro evolution of RNA biosensors (DRIVER) enables multiplexed discovery of biosensors. With DRIVER and high-throughput characterization (CleaveSeq) fully automated on liquid-handling systems, we identify and validate biosensors against six small molecules, including five for which no aptamers were previously found. DRIVER-evolved biosensors are applied directly to regulate gene expression in yeast, displaying activation ratios up to 33-fold. DRIVER biosensors are also applied in detecting metabolite production from a multi-enzyme biosynthetic pathway. This work demonstrates DRIVER as a scalable pipeline for engineering de novo biosensors with wide-ranging applications in biomanufacturing, diagnostics, therapeutics, and synthetic biology.