bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selected ‘Starter Kit’ energy system modelling data for Egypt (#CCG)
Authors: Allington Lucy , Cannone Carla , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Brown Edward , Howells Mark , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Wogan David , Barcelona Edito , Rogner Holger , Hirmer Stephanie 
Published: May 2021

Abstract
Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to starting energy system modelling in developing countries, thereby causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Egypt, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and two stylized scenarios (Fossil Future and Least Cost) for 2020-2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
A Comparative study on structural, morphological and photocatalytic properties of anodically grown ZnO nanowires under varying parameters
Authors: Ozdemir Erdem Tevfik , KARTAL Uğur , Dikici Tuncay , Erol Mustafa , Yurddaskal Metin 
Published: May 2021

Abstract
In this study, Zinc oxide (ZnO) nanowires (NWs) were successfully produced on Zn plates through electrochemical anodization in potassium bicarbonate aqueous electrolytes with different production parameters in two groups as applied voltage and anodization time. Subsequently, the ZnO NWs were annealed at 300 ℃ for 1 h in air atmosphere to increase crystallinity and remove organic residues. Structural and morphological properties were determined through X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis. The effect of anodization parameters on the structure of ZnO NWs and thus their photocatalytic activities were evaluated in detail by UV spectrophotometer. The results pointed out that, the most effective nanostructure on the photocatalytic degradation of methylene blue was obtained at the sample anodized under 30 V for 30 min at room temperature with 90.6% degradation efficiency among the samples. This result shows that the NW structured ZnO materials are promising to be used as effective photocatalysts in the removal of organic pollutants by solar energy and their conversion to green compounds.
MWCNT/Ti-doped ZnO nanocomposite as electrochemical sensor for detecting glutamate and ascorbic acid
Authors: Pitiphattharabun Siraprapa , Meesombad Kornkamon , Panomsuwan Gasidit , Jongprateep Oratai 
Published: May 2021

Abstract
Attributed to high stability and reproducibility, non-enzymatic electrochemical sensors have been extensively utilized in detection of various chemical substances. In this study, multi-walled carbon nanotube (MWCNT)/Ti-doped ZnO composite was prepared for fabrication of working electrode used in detection of glutamate and ascorbic acid. Ti-doped ZnO particles with the average size of 44.47 ± 4.05 nanometers were synthesized by the solution combustion technique. Hydrothermal impregnation was employed in preparation of the MWCNT/Ti-doped ZnO composite. Electrocatalytic activities of the MWCNT/Ti-doped ZnO composite were examined using cyclic voltammetry technique. The cyclic voltammograms revealed the reduction reaction of glutamate close to -0.5 V, whereas the reduction reaction of ascorbic acid close to 0.05. Fair electrocatalytic performance, with acceptable sensitivity, limit of detection and selectivity suggested potential utilization of MWCNT/Ti-doped ZnO nanocomposite in detection of ascorbic acid and glutamate.
Roles of Multimodal Intra-Operative Neurophysiological Monitoring (IONM) in Percutaneous Endoscopic Transforaminal Lumbar Interbody Fusion: A Case Series of 113 Patients
Authors: chen yu , Chun-Mei Luo , Juan Wang , Libangxi Liu , Bo Huang , Chang-Qing Li , Yue Zhou , Chencheng Feng 
Published: May 2021

Abstract
Background: Despite the widely used intra-operative neurophysiological monitoring (IONM) in spinal surgeries, the efficacy of IONM during percutaneous endoscopic transforaminal lumbar interbody fusion (PE-TLIF) surgery in detecting post-operative neurological deficits has not been well characterized.Objective: To investigate the efficacy and specificity of multimodal IONM (MIONM) on the detection of neurological complications during PE-TLIF surgeries.Methods: Multimodal IONM data from 113 consecutive cases who underwent PE-TLIF surgeries betweenJune2018and April 2020 were retrospectively reviewed. Post-operative neurological deficits were documented and analyzed, and the efficacy and specificity of various IONM techniques were compared.Results: Of the 113 consecutive patients, we identified 12 (10.6%) IONM alerts. The multimodal IONM sensitivity and specificity were 100% and 96.2%, respectively. The frequency of neurological complications, including minor deficits, was 6.2% (n = 7), all of which were temporary. Analysis of single IONM modalities varied between 25.0% and 66.6% with respect to their abilities to detect neurological complications compared to 100% when using all modalities.Conclusions: Multimodal IONM is more effective and accurate in assessing nerve root function during PE-TLIF surgeries, reducing both neurological complications and false-negative findings compared to unimodal monitoring. We recommend multimodal IONM in PE-TLIF surgeries.
Periprosthetic Joint Infection – Comparison of Automated Multiplex-PCR Unyvero i60 ITI Cartridge System with Bacterial Culture and Real-Time PCR
Authors: Luedemann Martin , Sulastyanto Saskia , Raab Peter , Schoen Christoph , Rudert Maximilian 
Published: May 2021

Abstract
Purpose: The evaluation of an automated-multiplex-PCR cartridge system for patients with suspicion of a periprosthetic joint infection (PJI) in comparison with conventional microbiological culture and 16S-rDNA-PCR.Methods: On suspicion of PJI synovial fluid specimen were taken preoperatively or periprosthetic tissue was collected intraoperatively. Microbiological analysis included conventional culture, 16S-rDNA-PCR and automated-multiplex-PCR (Unyvero-i60-ITI®). The European Bone and Joint Infection Society (EBJIS) criteria were used for PJI diagnosis. Positive and negative percent agreement was calculated. Total percentage agreement and Cohen`s kappa coefficient were calculated. Sensitivity, specificity and positive predictive value of conventional culture, 16S-rDNA-PCR and multiplex-PCR were calculated. 10 specimens of proved PJI were used as control group.Results: 50 specimen were suitable for culture. 14 (28%) were classified as PJI, 36 (72%) were aseptic. Coagulase-negative staphylococci was the most frequent detected pathogen. Concordance-rate between mPCR and culture results was 75.6 % with a Cohen`s kappa of 0.28. Concordance-rate between mPCR and 16S-rDNA was 82.9%, Cohen`s kappa was 0.13. Concordance analysis between culture results and 16S-rDNA lead to a concordance-rate of 88.9%. Cohen`s kappa was calculated with 0.6. With regard to the microbiological culture as reference, sensitivity of the mPCR was 0.33 and specificity was 0.91. Sensitivity and specificity of the 16S-rDNA-PCR was 0.55 and 0.97. The positive predictive value was 0.57 for the mPCR and 0.83 for the 16S-rDNA-PCR.Conclusion: Due to fair agreement between mPCR and conventional microbiological culture, the tested multiplex-PCR could be an additional instrument for the detection of PJI but is not superior over the conventional culture.
Long Noncoding RNA FER1L4 Promotes the Malignant Processes of Papillary Thyroid Cancer by Targeting miR-612/ Cadherin 4 Axis
Authors: Wu Luyao , Ding Yu , Tong Houchao , Zhuang Xi , Cai Jingsheng , Si Yan , Zhang Hao , Wang Xiaoting , shen meiping 
Published: May 2021

Abstract
Background: Long noncoding RNAs (lncRNAs) have emerged as crucial regulators in various cancers. However, the functional roles of most lncRNA in papillary thyroid cancer (PTC) are not detailly understood. This study aims to investigate the biological functions and molecular mechanisms of lncRNA Fer-1 like family member 4 (FER1L4) in PTC.Methods: The expression of FER1L4 in PTC was determined via operating qRT-PCR assays. Meanwhile, the clinical significance of FER1L4 in PTC patients was described. The biological functions of FER1L4 on PTC cells were evaluated by gain and loss of function experiments. Moreover, animal experiments were performed to reveal the effect on tumor growth. Subcellular distribution of FER1L4 was determined by fluorescence in situ hybridization and subcellular localization assays. Luciferase reporter assay and RNA immunoprecipitation assay were applied to define the relationship between FER1L4, miR-612, and Cadherin 4 (CDH4). Results: Upregulated expression of FER1L4 in PTC tissues was positively correlated with lymph node metastasis (P = 0.020), extrathyroidal extension (P = 0.013) and advanced TNM stages (P = 0.013). In addition, knockdown of FER1L4 suppressed PTC cell proliferation, migration and invasion, whereas ectopic expression of FER1L4 inversely promoted these processes. Mechanistically, FER1L4 could competitively bind with miR-612 to prevent the degradation of its target gene CDH4. This condition was further confirmed in the rescue assays.Conclusions: This study first demonstrates FER1L4 plays an oncogenic role in PTC via a FER1L4-miR-612-CDH4 axis and may provide new therapeutic and diagnostic targets for PTC.
Immune Cell Infiltration Score and Predicting Model Among Lower-grade Gliomas Based on Immunogenomic Clusters
Authors: Wang Zhile , Xie Fucun , Wu Yijun , Wang Li , Bai Yi , Long Junyu , Wang Xiang 
Published: May 2021

Abstract
Background and objectives: Recurrent malignancies had become a significant problem for the treatment of gliomas. Though immunotherapy was regarded as a possible solution, verification of immune-checkpoint inhibitors in multiple clinical cases failed. We aimed to explore target genes for immunotherapy and evaluate the genes through new scoring criteria.Methods: We firstly classified the patients through k-means clustering in immune cell level and gene level. Differential prognostic genes were weighted through principal component analysis (PCA) and Boruta algorithm. The comprehensive scoring of each component was defined as the ICI score. We further analyzed the relationship between ICI score and various clinical factors and prognosis. Moreover, a nomogram based on the ICI scores was built and validated through both internal and external validation.Results: The study cohort finally enrolled 495 patients. We identified a list of differential genes of which the expression level was closely related to prognosis. ICI scores were calculated for each case according to the expression level of prognostic genes. The cases in high ICI score group showed significantly lower survival probabilities (log-rank test; p<0.001). We further built a nomogram model based on the ICI scores. The area under the receiver operating characteristic (ROC) curve (AUC) values of the nomogram were 0.851, 0.86, and 0.768 in internal validation, and 0.725, 0.744, and 0.735 for 1-year, 3-year and 5-year truncation time in external validation.Conclusions: The differential genes were listed for further studies. ICI score had been confirmed to be closely related to prognosis and even genome instability. And the nomogram model based on ICI scores showed the feasibility in clinical practice in both internal and external validation.
Health Information-Seeking Behaviors of a German Speaking Minority in Italy: Latent Class Analysis of a Population-Based Cross-Sectional Survey
Authors: Ausserhofer Dietmar , Wiedermann Wolfgang , Wiedermann Christian J. , Becker Ulrich , Vögele Anna , Piccoliori Giuliano , Engl Adolf 
Published: May 2021

Abstract
Latent classes of health information-seeking behaviors of adults in a German-speaking minority of Italy were explored in a population-based, telephone survey on 10 health information sources conducted in South Tyrol, Italy. Data were collected from 504 adults (primary language German 68%, Italian 28%) and analyzed using latent class analysis and latent class multinomial logistic regression models. Three classes of health information-seeking behaviors emerged: “multidimensional” (23.3%), “interpersonal” (38.6%) and “technical/online” (38.1%). Compared to the “technical/online” class, “interpersonal” class members were older, had lower education than high school, and were less likely to be of Italian ethnicity. “Multidimensional” class members were more likely to be female, older, and of German ethnicity than those in the “technical/online” class. Linguistic ethnicity explains membership in classes of health-information-seeking behaviour. Policy makers and healthcare providers need to consider the health information-seeking behaviors of population subgroups to promote the health literacy skills of language minority groups.
Effect of Tumour Size Ratio on Liver Recurrence-free Survival of Patients Undergoing Hepatic Resection for Colorectal Liver Metastases
Authors: Zhai Yana , Bai Weijun , Zhou Jin , Dong Qian , Zhang Jingdong 
Published: May 2021

Abstract
Purpose: The study aimed to assess the impact of size differences of multiple liver metastases on liver recurrence-free survival (RFS) in patients undergoing hepatic resection for colorectal liver metastases (CRLMs). Methods: Overall, 147 patients with CRLMs who underwent hepatic resection between January 2007 and December 2016 were retrospectively analysed. Based on the tumour size ratio (TSR), patients were classified into three groups: TSR <2, 2≤TSR<4, and TSR ≥4. The differences in the liver RFS across the groups were analysed using the Kaplan-Meier method.Results: According to the multivariate analysis, TSR of 2–4 (hazard ratio [HR], 2.580; 95% confidence interval [CI] 1.543–4.312; P <0.001) and TSR <2 (HR, 4.435; 95% CI 2.499–7.872; P <0.001) were associated with worse liver RFS. As TSR decreased, liver RFS worsened. The Tumour Burden Score (TBS) is a prognostic tool, and TSR could further stratify patients in zones 1 and 2 into different risk groups according to the TBS criteria (zone 1: median liver RFS, 3.2 and 8.9 months for groups 1 and 2, respectively, P = 0.003; zone 2: median liver RFS, 3.5, 5.0, and 10.9 months for groups 1, 2, and 3, respectively, P <0.05). The differentiation ability of the new clinical score, which includes TSR, was superior to that of the Fong clinical score.Conclusion: TSR, as a prognostic tool, could accurately assess the effect of size differences across multiple liver metastases on liver RFS in patients undergoing hepatectomy for CRLMs.
Validation of Reference Genes for Studying Different Abiotic Stresses by RT-qPCR in Oat (Avena Sativa L.)
Authors: Tajti Judit , Pál Magda , Janda Tibor 
Published: May 2021

Abstract

Background: Oat (Avena sativa L.) is a widely cultivated cereal with high nutritional value growing mainly in temperate regions. The number of studies dealing with gene expression changes in oat increases and to obtain reliable RT-qPCR results it is essential to use references genes that are least influenced by experimental conditions. However, no detailed study was conducted on reference genes in different tissues of oat under diverse abiotic stress conditions. Results: In our work 9 candidate reference genes (ACT, TUB, CYP, GAPD, UBC, EF1, TBP, ADPR, PGD) were chosen and analysed by 4 statistical methods (GeNorm, Normfinder, BestKeeper, RefFinder). Samples were taken from two tissues (leaves and roots) of 13-day-old oat plants exposed to 5 abiotic stresses (drought, salt, heavy metal, low and high temperatures). ADPR was the top-rated reference gene for all samples, while different genes proved to be the most stable depending on tissue type and treatment combinations. TUB and EF1 were most affected by the treatments in general. Validation of reference genes was carried out by PAL expression analysis which further confirmed their reliability. Conclusions: Our main goal was to identify reference genes with stable expression in oat under different abiotic stress conditions in different tissues. These results can contribute to reliable gene expression studies for future researches in cultivated oat.
Association between ChAdOx1 nCoV-19 vaccination and bleeding episodes: large population-based cohort study
Authors: Trogstad Lill , Robertson Anna Hayman , Mjaaland Siri , Magnus Per 
Published: May 2021

Abstract
Objective To compare prevalence of skin, nose and gingival bleedings after receipt of adeno-vectored or mRNA-vaccines against covid-19. The hypothesis is that milder symptoms indicating altered thrombocyte function may affect a larger proportion of vaccinated individuals than the recently reported severe cases with thrombosis and thrombocytopenia.Design Large, population-based cohort study.Setting Norway, nationwide.ParticipantsMore than 80 000 cohort participants responding to electronic questionnaires about covid-19 vaccination and potential side effects during weeks 11-13, 2021. Response rate 58% (81267/138924), 83% were female, 85% health care workers and 80% were aged 40-55 years. Main outcome measuresSelf-reported episodes of skin, nose and gingival bleedings. Variation in rates by registered vaccine type, age, sex, occupation, previous covid-19 infection and chronic disease. ResultsFour of the 3416 subjects (0.2%) who were vaccinated with a single dose of mRNA vaccine reported skin bleeding as a side effect, as opposed to 163 of 5132 subjects (3.2%) vaccinated with a single dose of the adenovirus-vectored vaccine, OR (odds ratio)=16.0 (95% confidence interval (CI) 7.5-34.1). Corresponding ORs for nose and gingival bleeding were 8.0 (4.0-15.8) and 9.3 (4.3-20.0), respectively.ConclusionsThese findings could potentially indicate that the adenovirus-vectored vaccine may lead to mild bleeding episodes in a larger proportion of vaccinated individuals, and not only in rare cases with documented thrombosis and thrombocytopenia. Studies are needed to understand the possible mechanisms behind these observations, and to establish or refute whether they share similarities with the severe thromboembolic bleeding complications.
Clinical Implementation of HyperArc
Authors: Wong Felix Hau Chun , Moleme Puleng Adelice , Ali Omer Abdelaziz , Mugabe Koki Victor 
Published: May 2021

Abstract
The aim of this study was to present our experience on clinical implementation of HyperArc including dosimetric comparison between VMAT and HyperArc plans and dosimetric verification of HyperArc. In this study, eleven previously treated cases of brain metastasis were selected from our brain stereotactic radiotherapy program. The cases were retrospectively planned using HyperArc technique and the plan quality was evaluated. In addition, dosimetric effects of HyperArc plan with different energies and using jaw tracking technique were evaluated. Furthermore, dosimetric verification of HyperArc plans was performed using ion chamber and radiochromic film. Our results of dosimetric comparison shows that HyperArc technique improved both conformity index and gradient index compared to VMAT plans. We also found that using 6MV flattening filter free (6MV-FFF) beam improves gradient index in HyperArc plans compared to using 6MV flattening filter beam. Furthermore, our results show that jaw tracking technique reduces the size of low dose volume while maintaining similar target coverage, conformity index, and gradient index. In our dosimetric verification study, results of ion chamber and film measurement indicate no significant difference between VMAT and HyperArc plans. In conclusion, HyperArc simplifies planning of stereotactic treatment for brain and improves the dosimetry in treatment plans. Additionally, HyperArc provides safe and efficient treatment delivery to stereotactic treatment to brain.
Decision Making Power Over Reproductive Health Service Utilization and Associated Factors Among Married Women in Dupa Town and Surrounding Rural Kebeles, Illuababora Zone, South West Ethiopia,2020
Authors:   Zerihun Bayabil1, Nigatu Dessalegn 
Published: May 2021

Abstract

Background

Decision making power of women is one of the essential factors which have influence on maternal health service utilizations. Women’s lack of decision over reproductive health service utilization affects their protection from unwanted child bearing, unsafe sex and their consequences.
Objective

The main objective of this study was to assess decision-making power on RH-service utilization and its associated factors among married women in Illuababor zone, Dupa town and surrounding rural kebeles, South West Ethiopia, 2018.
Methods

Community based comparative cross-sectional study was conducted from May to July, 2018 among 584(urban 292 and rural 292) married women of reproductive age group. Women meet the inclusion criteria were interviewed by using structured and pre-tested questionnaire. The data were entered into EPI data version 3.1 and exported to SPSS version 20 computer software for analysis. Both Bivariate and multivariable logistic regression analysis was used. Level of significance was declared at a p value of < 0.05
Results

A total of 288 in urban and 288 in rural married women were interviewed and yields a response rate of 98.6% both in urban and rural. Decision-making power in urban and rural was 55.2% [95% CI (52.7–64.6)] and 40.3% [95% CI (39.9–52.5)] respectively. In urban, being wives of government employed spouses [AOR 2.102 95% CI (1.16, 3.81)], knowledge on RH [AOR 3.33, 95% CI (1.20, 12.49)], above five years in marriage [AOR 1.91, 95% CI (1.19, 7.70)], were found to be predictors of women’s decision-making power.
Interpretations &Conclusion:

Urban women had better power to make decisions than rural on reproductive health service utilization. Reproductive health interventions in the area should be promoted by considering empowering of married women on reproductive health service utilization.
CD4+ T Cells of Prostate Cancer Patients Have Decreased Immune Responses to Antigens Derived from SARS-CoV-2 Spike Glycoprotein
Authors: Taborska Pavla , Strizova Zuzana , Stakheev Dmitry , Sojka Ludek , Bartunkova Jirina , Smrz Daniel 
Published: May 2021

Abstract
The adaptive immune response to severe acute respiratory coronavirus 2 (SARS-CoV-2) is important for vaccine development and in the recovery from coronavirus disease 2019 (COVID-19). Men and cancer patients have been reported to be at higher risks of contracting the virus and developing the more severe forms of COVID-19. Prostate cancer (PCa) may be associated with both of these risks. We show that CD4+ T cells of SARS-CoV-2-unexposed patients with hormone-refractory (HR) metastatic PCa had decreased CD4+ T cell immune responses to antigens from SARS-CoV-2 spike glycoprotein but not from the spiked glycoprotein of the 'common cold'-associated human coronavirus 229E (HCoV-229E) as compared with healthy male volunteers who responded comparably to both HCoV-229E- and SARS-CoV-2-derived antigens. Moreover, the HCoV-229E spike glycoprotein antigen-elicited CD4+ T cell immune responses cross-reacted with the SARS-CoV-2 spiked glycoprotein antigens. PCa patients may have impaired responses to the vaccination, and the cross-reactivity can mediate antibody-dependent enhancement (ADE) of COVID-19. These findings highlight the potential for increased vulnerability of PCa patients to COVID-19.