bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosomal Concordance between the Baby Produced by Preimplantation Genetic Testing Embryo and the Trophectoderm Biopsy: a Retrospective Study
Authors: Yao Zhongyuan , Wang Xiaoxia , Zeng Jun , Zhao Jing , Xia Qiuping , Zhang Lei , Wu Lingqian , Li Yanping 
Published: March 2021

Abstract
BackgroundChromosomal mosaicism and aneuploidies are routine phenomena throughout human pre- and post-implantation development. The benefit of implanting mosaicism or aneuploidies is still controversial. The purposes of the study are to investigate the developmental potential of embryos with chromosomally segmental or mosaic abnormalities, and whether precise Next Generation Sequencing (NGS) resolution would reduce the development of an abnormal embryo in preimplantation genetic testing (PGT) cycles.MethodsThe peripheral blood of 17 PGT babies were collected for single nucleotide polymorphism (SNP) array and were compared with trophectoderm (TE) biopsy results at different NGS resolutions.Results76.5% (13/17) of babies’ peripheral blood chromosome analysis was consistent with 10Mb TE biopsies and 58.8% (10/17) of babies’ analysis was consistent with 4Mb TE biopsies. 2 babies who had euploid TE showed abnormal peripheral blood chromosome analysis. 17.6% (3/17) embryos with aberrant TE biopsies produced healthy babies. Although the sensitivity of 10Mb was lower than 4Mb (25% vs. 50%), the specificity (100% vs. 76.9%), PPV (100% vs. 40%) and diagnostic accuracy (82.4% vs. 70.6%) of 10Mb showed better results than 4Mb.Conclusion(s)The chromosomal results between peripheral blood samples and TE biopsies of born babies are not completely congruent. Aneuploid and mosaic embryos have potential to produce healthy babies, whereas normal embryos also have chance to produce babies with chromosomal abnormalities. In spite of low sensitivity of both resolutions, 10Mb has higher specificity, PPV and diagnostic accuracy than 4Mb. It is suggested that TE biopsy be analyzed in both 10Mb and 4Mb resolutions to uncover severely adverse chromosomal aberrations but use 10Mb resolution to guide transfer.Trial registrationThe study was retrospectively registered in the Chinese Clinical Trial Registry (ChiCTR2100042522).
Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways
Authors: Behroozi Zahra , Ramezani Fatemeh , Nasirinezhad farinaz 
Published: March 2021

Abstract
Background: There are complex mechanisms for reducing intrinsic repair ability and neuronal regeneration following spinal cord injury (SCI). Platelet-rich plasma (PRP) is a rich source of growth factors and has been used to stimulate regeneration of peripheral nerves in degenerationtive diseases. However, only a few studies have investigated the effects of PRP on the SCI models. We examined whether PRP derived from human umbilical cord blood (HUCB-PRP) could recover motor function in animals with spinal cord injury. We also investigate the role of Wnt signaling pathway.Methods: Ault male Wistar rats were randomly divided into 6 groups (n=60) as control, sham, SCI, vehicle (SCI+platelet-poor plasma), PRP2day (SCI+injection 2 days after SCI) and PRP14day (SCI+injection 14 days after SCI). SCI was performed at the T12-T13 level. BBB tests were done weekly after injury for six weeks. caspase3 expression was determined using the Immunohistochemistry technique. The expression of GSK3β, Tau and MAG were determined using the Western blot technique. Data were analyzed by PRISM & SPSS software. Results: PRP injected animals showed a higher locomotor function recovery than those in the SCI group (p<0.0001). The level of caspase3, GSK3β and CSF- Tau reduced and MAG level in the spinal cord increased by injection of HUCB-PRP in animals with spinal cord injury. Conclusions: Injection of HUCB-PRP enhanced hind limb locomotor performance by modulation of caspase3, GSK3β, tau and MAG expression. Using HUCB-PRP could be a new therapeutic option for recovering the motor function and axonal regeneration after spinal cord injury.
Design And Application Of Iot Based Real-Time Patient Telemonitoring System Using Biomedical Sensor Network
Authors: Debnath Prama , Mahmud Apple , Hossain Ahsanul Kabir , Rahman S M Imrat 
Published: March 2021

Abstract
The field of Internet of Things (IoT) and Remote Patient Monitoring (RPM) system has been developing rapidly causing these technologies to be adopted in multiple domains all around the world. This paper presents the design of a real-time remote monitoring system for vulnerable patients. The proposed device measures body temperature, heart rate, blood pressure, Oxygen saturation level and electrocardiogram signals (ECG) of patients admitted into Intensive Care Unit (ICU) or Operating Room (OR), and automatically updates all these data on a corresponding hybrid cloud server in every 45 seconds to which a corresponding physician has access. More than 2000 cycles are conducted within a day where all five biomedical parameters are measured and stored on each cycle. Furthermore, the system includes alarm triggering system and emergency Short Message Service (SMS) alert service using GSM technology in order to initiate proper control actions in case of emergencies. This device facilitates doctors, nurses, relatives of patients, or anyone around the world to monitor real-time parameters of patients. The process was tested on 10 patients obtaining a satisfactory outcome. The system proposed in this paper reveals a new vision of biomedical engineering which tends to the era of remotely controllable ICU environment with more interactive, efficient and fast response of both patient and corresponding physicians.
MULTI-CRITERIONAL CHOICE OF AN ALTERNATIVE UNDER THE RULES OF FUZZY PRODUCTS WITH SOME RELIABILITY DEGREE
Authors: Habibov Ibrahim Abulfaz , Dyshin Oleg , Аghаmmadova Sevda Alipasha , Hasanzade Irada Sabir 
Published: March 2021

One of the main problems of decision-making tasks is the need to take into account subjective expert assessments, the complete consistency of which is rare, and the choice of the best alternative. The complexity of the connections between the many-sided aspects of the decision-making situation and the lack of an accurate forecast of the consequences leads to the fact that when assessing and choosing alternatives, it is possible, and often necessary, to use and process qualitatively fuzzy estimates. In decision-making situations, when at least one of the elements (outcomes, criteria, preferences, expert opinions, etc.) is described qualitatively, indistinctly, there are problems of multi-criteria decision-making with fuzzy initial information.
Let’s consider the solution to the problem of multi-criteria choice based on the rules of fuzzy conditional inference, which have the form of fuzzy statements, the conditions and conclusions of which, along with expert assessments of the criteria, are presented in the form of interval fuzzy numbers of the second type (IT2FN). The convolution of private implications in each statement is made according to Lukasiewicz's rule. To reduce the type and defuzzify the resulting IT2FN, the Karrnik-Mendel algorithm was used to construct the minimum and maximum centroids of nested fuzzy sets of the first type, which give an estimate of the utility interval for each alternative. To refine the obtained utility estimates, under conditions of incomplete definiteness of statements, using the generalized Bayesian inference mechanism, adjusted estimates of the utility intervals of alternatives are constructed. By comparing these intervals, a larger interval is determined and the corresponding alternative is taken as a solution to the problem under consideration.
The application of the proposed approach to solving the problem of multicriteria selection of the most corroded section of a gas pipeline with ambiguous expert opinions is shown. To date, specific practical and theoretical results have been obtained for decision-making problems with fuzzy initial information
Photocatalytic degradation of hydrocarbons and methylene blue using floatable titanium dioxide catalysts in contaminated water
Authors: Schnabel T. , Jautzus N. , Mehling S. , Springer C. , Londong J. 
Published: March 2021

Abstract
Photocatalytic disintegration is a novel approach to eliminate pollution. The method utilizes the semiconductor titanium dioxide to degrade organic molecules in the presence of ultraviolet (UV) light. In this study, it is shown how the capabilities of several types of catalyst designs degrade the non-polar substance diesel fuel and the polar substance methylene blue. The floating design of foam glass coated with titanium dioxide could reduce the concentration of diesel fuel by 329 mg/L in 16 h; the submerged designs for coated glass fiber and coated steel grit could reduce methylene blue concentration by 96.6% after 4 h and 99.1% after 6 h, respectively. It could be shown that photocatalysis is a promising cost- and energy-efficient method for managing air and water pollution. It can be established as a low-technology method without requiring the use of a conventional source of energy, given an adequate amount of sun hours, or as an additional cleaning stage in water treatment plants using UV-LEDs.
Lipid Lowering Natural Products Targeting hPCSK9 May Prevent the Severity of COVID-19 Infection
Authors: Basak Ajoy , Vaijanathappa Jaishree , Basak Sarmistha 
Published: March 2021

Background:
The deadly outbreak of COVID-19 disease caused by novel SARS CoV2
has created an unprecedented global health crisis affecting every sectors of human life and enormous
damage to world’s economy. With >16.1 million infections and >650,000 deaths worldwide
as of July 27, 2020, there is no treatment for this disease neither is there any available vaccine. Serious
research efforts are ongoing on all fronts including treatment, prevention and diagnosis to combat
the spread of this infection. A number of targets that include both viral and host proteins have
been identified and became part of intense investigation. In this respect the viral surface spike (S)
glycoprotein caught the attention most. It is cleaved by multiple host proteases to allow recognition
by host receptor human Angiotensin Converting Enzyme2 (hACE2) leading to fusion and viral replication.
Natural products, small compounds, antioxidants, peptides, proteins, oligonucleotides,
antibodies and other compounds are under investigation for development of antiviral agents against
COVID- 19.

Objective:
Recently cholesterol lowering phytocompounds Quercetin, Swertiamarin and Berberine
which promote human Low Density Lipoprotein Receptor (hLDLR) via inhibition of human Proprotein
Convertase Subtilisin Kexin9 (hPCSK9) have been shown to block viral infections caused
by ebola, influenza, Respiratory Syncytial Virus (RSV), Hepatitis C virus (HCV) and other RNA
type viruses. Since SARS CoV2 is a RNA virus with similar genetic structure and infection machinery,
it is hypothesised that these phytocompounds may also exhibit antiviral property against
COVID-19.

Methods:
Our above concept is based on recently published studies as well as our herein presented
in silico modeling and computational data which suggested strong interactions of hPCSK9 with
above phytocompounds and most importantly with hACE2 following its complexation with receptor
binding domain (RBD) of SARS CoV2 S protein.

Results:
These results and a proposed schematic model showing association of hPCSK9 with
SARS CoV2 infection are presented in this manuscript. It is proposed that hPCSK9 plays the role
of a co-receptor in binding with hACE2:RBD complex and thereby facilitates viral fusion.

Conclusion:
Our studies suggest that PCSK9 inhibitors may provide beneficial effect against
COVID-19 infection by retarding viral fusion through displacement of bound hPCSK9 from its
complex with ACE2:RBD of SARS CoV2 S protein.
Socioeconomic Disparities in eHealth Literacy and Preventive Behaviors During the COVID-19 Pandemic in Hong Kong: Cross-sectional Study
Authors: Guo Ziqiu , Zhao Sheng Zhi , Guo Ningyuan , Wu Yongda , Weng Xue , Wong Janet Yuen-Ha , Lam Tai Hing , Wang Man Ping 
Published: March 2021

Background
eHealth literacy can potentially facilitate web-based information seeking and taking informed measures.

Objective
This study aimed to evaluate socioeconomic disparities in eHealth literacy and seeking of web-based information on COVID-19, and their associations with COVID-19 preventive behaviors.

Methods
The COVID-19 Health Information Survey (CoVHIns), using telephonic (n=500) and web-based surveys (n=1001), was conducted among adults in Hong Kong in April 2020. The Chinese eHealth literacy scale (eHEALS; score range 8-40) was used to measure eHealth literacy. COVID-19 preventive behaviors included wearing surgical masks, wearing fabric masks, washing hands, social distancing, and adding water or bleach to the household drainage system. Adjusted beta coefficients and the slope indices of inequality for the eHEALS score by socioeconomic status, adjusted odds ratios (aORs) for seeking of web-based information on COVID-19 by socioeconomic status, and aORs for the high adherence to preventive behaviors by the eHEALS score and seeking of web-based information on COVID-19 were calculated.

Results
The mean eHEALS score was 26.10 (SD 7.70). Age was inversely associated with the eHEALS score, but education and personal income were positively associated with the eHEALS score and seeking of web-based information on COVID-19 (for all, P for trend<.05). Participants who sought web-based information on COVID-19 showed high adherence to the practice of wearing surgical masks (aOR 1.56, 95% CI 1.15-2.13), washing hands (aOR 1.33, 95% CI 1.05-1.71), social distancing (aOR 1.48, 95% CI 1.14-1.93), and adding water or bleach to the household drainage system (aOR 1.67, 95% CI 1.28-2.18). Those with the highest eHEALS score displayed high adherence to the practice of wearing surgical masks (aOR 3.84, 95% CI 1.63-9.05), washing hands (aOR 4.14, 95% CI 2.46-6.96), social distancing (aOR 2.25, 95% CI 1.39-3.65), and adding water or bleach to the household drainage system (aOR 1.94, 95% CI 1.19-3.16), compared to those with the lowest eHEALS score.

Conclusions
Chinese adults with a higher socioeconomic status had higher eHealth literacy and sought more web-based information on COVID-19; both these factors were associated with a high adherence to the guidelines for preventive behaviors during the COVID-19 pandemic.
PERCEIVED EXERTION AND MODERATE TO VIGOROUS PHYSICAL ACTIVITY IN MIDDLE SCHOOL STUDENTS ACCORDING TO THE PHYSICAL EDUCATION TEACHERS’ EXPERIENCE
Authors: Ochoa-Martínez Paulina Yesica , Hall-López Javier Arturo , López Campos Cristhian Emmanuel , Alarcón Meza Edgar Ismael 
Published: March 2021

The aim of the current study is to compare the perceived exertion and moderate to vigorous physical activity (PA) among first grade middle school students in physical education (PE) classes given by PE teachers with higher or lower experience than 16 years. Eighty-seven PE lessons conducted by PE teachers with ≥ 16 years of experience (n=35) and teachers with ˂ 16 years of experience (n=52) were evaluated by 348 middle school students (Mean age=12.2±0.5 years). To determine the perceived exertion, the pictorial children's effort rating table (PCERT) was completed, after PE. Children chose an option on an illustrated scale 1–10 perceived exertion. The moderate to vigorous physical activity (MVPA) was evaluated using the System for Observing Fitness and Instruction Time (SOFIT) assessing the intensity as the proportion of time that students spent engaged in walking and very active PA during PE. A cross-sectional design was used, the equality of variance was calculated using the Student t-test for independent samples resulting in MVPA (P-value=.659) and perceived exertion (P-value=.205) α≤ than 0.05. In both groups, the average value of perceived exertion was less than 5 points on the scale, which is insufficient to enhance physical fitness. The MVPA did not achieve international guidelines of engaging students in at least 50% of the lessons time in walking and very active PA. It is recommended for PE teachers to develop pedagogical strategies contributing from the school environment to guide students in educational contents for health through PA, as established by UNESCO and recommended by the World Health Organization.
PHYSICAL FITNESS OF BOYS PERTAINING TO UNDERWEIGHT, OVERWEIGHT AND OBESITY
Authors: Wasiluk Agnieszka , Saczuk Jerzy 
Published: March 2021

Together with the changing socio-economic status of the Polish society, differences in the nutritional status of children and youth and their lower physical fitness levels are becoming more noticeable. The aim of this research was to define changes that occurred over a 10-year period regarding physical fitness of boys with proper weight-height proportions and of their underweight, overweight and obese counterparts. In 2004/05, somatic features and physical fitness were examined in 16116 and 9507 boys, respectively, aged 10-18. Ten years later somatic features and physical fitness assesmensts were repeated in 6972 and 6834 boys aged 10 to 18. The participants performed Eurofit tests and their body height and body mass were measured in order to calculate the Body Mass Index (BMI). The ten-year differences in physical fitness were expressed on a T point scale, while their extent was estimated with the Student’s t-test for independent data. The frequency of occurrence of underweight and overweight was expressed in percentage values and verified with the use of the χ² test. In the analysed decade, an increase in the frequency of occurrence of both overweight and serious underweight was noted. It resulted in a lower level of physical fitness in children and youth belonging to the aforementioned groups. The most serious negative changes were noted in boys aged 13-15. It can be concluded that unless remedial programmes are implemented, the young generation will experience biological deterioration in the decades to come, which may result in an epidemic of lifestyle diseases in younger and younger individuals.
A COMPARATIVE OVERVIEW OF DATABASE MANUFACTURERS’ PROTECTION OF RIGHTS IN THE CROATIAN AND SERBIAN LAWS
Authors: Ljubojev Nadežda , Dukić Mijatović Marijana 
Published: March 2021

This paper analyzes the right of a database manufacturer which is the exclusive right that authorizes its holder to prohibit or permit another to use a particular database, i.e. the collection of regulated and systematized data, copyright works or other materials that are available electronically or otherwise. However, the database can be a collection in terms of the copyright of the collection, so it also represents the content of the analysis in this paper. The subject of comparative analysis are the provisions of the current regulations in the Republic of Croatia and the Republic of Serbia, and determining their compliance with the Directive 96/9/EC on the legal protection of databases. The analysis used a historical legal and normative legal method. Based on the analysis, the authors point out that the adopted solutions for the protection of databases in the EU greatly influenced the adoption of the corresponding regulations in the Republic of Serbia, concluding that the regulations governing this area in the Republic of Serbia are almost completely aligned with the EU regulations.
Anthropometric indicies of infants with realized perinatal transmission of HIV
Authors: Daminov T. A. , Tuychiev L. N. , Akhmedova M. D. , Khudaykulova G. K. , Rakhmatullaeva Sh. B. 
Published: March 2021

Purpose of the study: evaluation of the anthropometric parameters of newborns with realized perinatal transmission of HIV.Materials and methods: The article presents the analysis of the main anthropometric parameters (length, weight, head and chest circumference) of 147 infants born to HIV-infected mothers who were divided into 2 groups of observation (with realized and unrealized transmission of HIV).Results: These results demonstrate that a reduction in comparison with the normative parameters of anthropometric indicators such as body weight, head circumference and chest circumference at birth, is a distinctive feature, substantiating intrauterine infection with HIV. Indicators body length did not significantly deviate from the norm in both groups observation that, apparently, was associated with a short-term impact of HIV on the child body during pregnancy.
Non-invasive methods for the diagnosis of liver fibrosis in chronic HCV-infection
Authors: Fazul’zyanova A. I. , Husainova A. K. , Tkacheva S. V. , Yakupova F. M. 
Published: March 2021

Objective: to study the values of fibrosis indices and transient elastometry in patients with chronic HCV infection who received antiviral therapy.Materials and methods: The study included 64 patients with chronic HCV infection who received antiviral therapy with direct-acting antiviral drugs or a combination of peginterferon and ribavirin.The fibrosis indices AAR, APRI and FIB-4 were calculated before the start of therapy and 6 months after its completion. Values of AAR>1, APRI≥1,5, and FIB-4≥1,45 were considered indicators of severe fibrosis. We studied the dynamics of fibrosis indices and elastometry values depending on the treatment regimen, their correlation and the prognostic significance of fibrosis indices in relation to elastometry.Results. Among patients treated with direct-acting antiviral drugs, a sustained virologic response was achieved in 100%, and peginterferon-containing regimen – in 85%. Elastometry and APRI and FIB-4 indices decreased in both groups. In patients without liver cirrhosis, the average elastometry after treatment decreased from 9,5±1,7 kPa to 6,7 ± 1.4 kPa (p = 0,0006). In patients with liver cirrhosis, the median of elastometry decreased from 20 to 11,7 kPa (p = 0,0006), the median of APRI decreased from 2,09 to 0,61 (p = 0,005), FIB-4 from 3,95 up to 2,22 (p = 0,022). The prognostic significance of FIB-4 in relation to elastometry before treatment was 81%, after – 82%.Conclusion. Successful etiotropic therapy leads to an improvement in values of liver fibrosis indices and transient elastometry in patients with HCV infection, including liver cirrhosis, regardless of the treatment regimen. The FIB-4 index showed the highest sensitivity and prognostic significance in determining severe fibrosis.