bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Early F-18 Florbetaben PET/CT to Tc-99m ECD SPECT Using Voxel, Regional, and Network Analysis
Authors: Kwon Soo Jin , Ha Seunggyun , Yoo Sang-Won , Shin Na-Young , O Joo Hyun , Yoo Ie Ryung , Kim Joong-Seok 
Published: May 2021

Abstract
This study aimed to validate early-phase F-18 Florbetaben positron emission tomography (eFBB PET) as a brain perfusion test and determine the optimal reference region. A total of 27 patients with early Parkinson’s disease with ethyl cysteinate dimer single positron emission tomography (ECD SPECT) and FBB PET were included. Six reference regions, including whole brain (GN), pons, central white matter (CWM), whole cerebellum (WC), WC with brain stem (WC + B), and cerebellar gray matter (CG), were applied to obtain SUVR. Correlations of SUVRs between eFBB PET and ECD SPECT were calculated for each normalization method. Voxel-wise comparison of the two imaging studies was done with paired t-test. Pearson’s r for SUVRs were 0.791 for GN (p < 0.001), 0.422 for pons (p = 0.028), -0.005 for CWM (p = 0.982), 0.886 for WC (p < 0.001), 0.897 for WC + B (p < 0.001), and 0.904 for CG (p < 0.001), respectively. Early phase FBB PET had significantly higher voxel-wise SUVR in cerebral cortices, striatum, midbrain regions, and lower voxel-wise SUVR in white matter, concordantly in both GN and CG normalization method (FDR adjusted-p < 0.05). Our findings suggest that eFBB PET is a reliable perfusion test based on a high correlation of SUVR with ECD SPECT. High gray-to-white matter contrast would be another advantage of eFBB PET for clinical use.
Accelerated Life Reliability Evaluation of Grating Ruler for CNC Machine Tools Based on Competing Risk Model and Incomplete Data
Authors: Yang Haiji , Li Guofa , He Jialong , Ma Yupeng , Wang Liding , Zhang Wei 
Published: May 2021

Abstract
Grating ruler is the high-precision linear displacement sensor used in the servo control system of CNC machine tools. It is one of the key components affecting the machining accuracy of CNC machine tools. Due to the long life-time of grating ruler, and it will cost a lot of time and resources to evaluate its reliability level by traditional life test. At the same time, there are some incomplete data in the failure data, which will cause inaccurate evaluation results. In this paper, an accelerated life reliability evaluation method of grating ruler based on competing risk model and incomplete data is proposed. Firstly, according to the characteristics of grating ruler, the life distribution model based on competitive risk is established, and the representation method and conversion method of failure data considering incomplete data are proposed. Secondly, the accelerated life test system which can apply temperature and humidity accelerated stress is built, and the reliability level of JFT series closed absolute grating ruler is evaluated by the proposed method. Finally, the proposed method is compared with the single life distribution model to verify the effectiveness.
Learning dynamics by computational integration of single cell genomic and lineage information
Authors: Wang Shou-Wen , Klein Allon 
Published: May 2021

Abstract
A goal of single cell genome-wide profiling is to reconstruct dynamic transitions during cell differentiation, disease onset, and drug response. Single cell assays have recently been integrated with lineage tracing, a set of methods that identify cells of common ancestry to establish bona fide dynamic relationships between cell states. These integrated methods have revealed unappreciated cell dynamics, but their analysis faces recurrent challenges arising from noisy, dispersed lineage data. Here, we develop coherent, sparse optimization (CoSpar) as a robust computational approach to infer cell dynamics from single-cell genomics integrated with lineage tracing. CoSpar is robust to severe down-sampling and dispersion of lineage data, which enables simpler, lower-cost experimental designs and requires less calibration. In datasets representing hematopoiesis, reprogramming, and directed differentiation, CoSpar identifies fate biases not previously detected, predicting transcription factors and receptors implicated in fate choice. Documentation and detailed examples for common experimental designs are available at https://cospar.readthedocs.io/.
Short-term effect of PM 2.5 and O3 on non-accidental mortality and respiratory mortality in Lishui District , China
Authors: Chen Yuqi , Jiao Zhigang , Chen Ping , Fan Lijun , Zhou XuDan , Pu Yuepu , Du Wei , Yin Lihong 
Published: May 2021

Abstract
Background

In recent years, air pollution has become an imminent problem in China. Few studies have investigated the impact of air pollution on the mortality of middle-aged and elderly people. Therefore, this study aims to evaluate the impact of PM2.5 and O3 on non-accidental mortality and respiratory mortality of the middle-aged and elderly in Lishui district of China and provide the scientific basis for the prevention and control measures of air pollution.
Method:

Using daily mortality and atmospheric monitoring data from 2015 to 2019, we applied a generalized additive model with time-series analysis to study the association of PM2.5 and O3 exposure with daily non-accidental mortality and respiratory mortality in Lishui district of China. Using attributable risk to estimate the death burden attributable to short-term exposure to O3 and PM2.5。
Result

PM2.5 and O3 were associated with non-accidental and respiratory mortality. For every 10µg/m3 increased in PM2.5, non-accidental mortality increased 0.94% (95%CI: 0.05%-1.83%), and PM2.5 had a more significant impact on women. For every 10µg/m3 increased in O3, respiratory mortality increased 1.35% (95%CI: 0.05%-2.66%). and O3 had a more significant impact on men. Compared with single pollutant model, the impact of the two-pollutant model on non-accidental mortality and respiratory mortality slightly decreased. Besides, in summer and winter, O3 had a more obvious impact on non-accidental mortality. The Population Attributable Fractions of non-accidental mortality were 0.839% (95%CI:0.004–1.626%) for PM2.5 and the PAF of respiratory mortality were 0.135% (95%CI:0.005–0.263%) for O3.
Conclusion

PM2.5 and O3 could significantly increase the risk of non-accidental and respiratory mortality in middle-aged and elderly people in Lishui district, China. Exposing to air pollutants, men were more susceptible to O3 damage, and women were more susceptible to PM2.5 damage.
CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p
Authors: Yang Yang , Fan Xin , Nie Yunfei , Liu Donglei , Zhu Dengyan , Wu Kai , Zhang Yuan , Li Wenhua , Tian Xiangyu , Wang Huaqi , Fan Yuxia 
Published: May 2021

Abstract
Background: Circular RNAs (circRNAs) act pivotal roles in the progression of multiple malignancies. However, the underlying mechanisms by which hsa_circ_0007031 (circTUBGCP3) contributes to lung adenocarcinoma (LAC) remain largely unknown.Methods: The association of circTUBGCP3 expression with clinicopathological characteristics and prognosis in patients with LAC was determined by RT-qPCR and fluorescence in situ hybridization. The in vitro functional experiments as well as a subcutaneous tumorigenesis model were executed to estimate the role of circTUBGCP3 in LAC cells. The interaction between circTUBGCP3 and miR-885-3p was confirmed by RNA immunoprecipitation, luciferase gene report and RT-qPCR assays. The effects of circTUBGCP3 on miR-885-3p-mediated Wnt10b/β-catenin signaling were evaluated by Western blot. Results: The upregulation of circTUBGCP3 or downregulation of miR-885-3p was associated with the pathological stage and poor survival in patients with LAC. Restored expression of circTUBGCP3 facilitated the growth and invasion of LAC cells, but knockdown of circTUBGCP3 harbored the opposite effects. In mechanism, circTUBGCP3 could act as a sponge of miR-885-3p, which suppressed the cell proliferation and colony formation and attenuated the tumor-promoting effects of circTUBGCP3. Wnt10b as a target of miR-885-3p could be upregulated be circTUBGCP3 and indicate poor survival in patient with LAC. Conclusions: Our findings demonstrated that circTUBGCP3 promoted LAC progression by sponging miR-885-3p, and might represent a prognostic factor for LAC.
Shaping Ability of Three Nickel-Titanium Rotary Instruments in Simulated L-Shaped Canals: Oneshape, Hero Shaper, and Revo-S
Authors: Vakili-Gilani Pooyan , Tavanafar Saeid , Saleh Abdulrahman Mohammad 
Published: May 2021

Abstract
Background
This study aimed to compare the shaping ability of OneShape, Hero Shaper, and Revo-S instruments in simulated L-shaped canals.
Methods
forty-eight simulated L-shaped canals were prepared to an apical size of 25 using OneShape, Hero Shaper, and Revo-S (all from Micro-Mega SA, Besançon, France), (n = 16 canals/group) systems. The amount of resin removed after each canal's preparation was measured and compared after producing a composite image made from the superimposition of pre and post-instrumented canals. Canal aberrations and the preparation time were also recorded. The data were statistically analyzed by using ANOVA, Tukey, and Chi-square tests.
Results
One file fractured during instrumentation in Revo-S group. A significant difference was found at the apical end of the prepared simulated canal between the groups, with Revo-S showing the least amount of resin removal from the inner side of the canals and Hero Shaper showing the highest amount of resin removal from the outer side (P < 0.05). Regarding the total width of the canals after preparation, a significant difference was found between the groups at the apical end and the straight portion of the canals, and Revo-S removed the least amount of resin at the straight portion of the canals (P < 0.05). No statistically significant differences were found between the different instruments regarding canal aberrations' incidence (P > 0.05).
Conclusions
all of the files showed a tendency to straighten the canals, whereas OneShape files maintained the original canal curvatures well.
Impact of COVID-19 on the health of the general and more vulnerable population and its determinants: Health care and social survey-ESSOC, study protocol
Authors: Sánchez-Cantalejo Carmen , Rueda Mª del Mar , Saez Marc , Enrique Iria , Ferri-García Ramón , Fuente Miguel De la , Villegas Román , Castro Luis , Barceló Maria Antònia , Daponte-Codina Antonio , Lorusso Nicola , Cabrera-León Andrés 
Published: May 2021

Abstract
Background : This manuscript describes the rationale and protocol of a real-world data (RWD) study entitled Health Care and Social Survey (ESSOC , Encuesta Sanitaria y Social ). The study’s objective is to determine the magnitude, characteristics, and evolution of the COVID-19 impact on overall health as well as the socioeconomic, psychosocial, behavioural, occupational, environmental, and clinical determinants of both the general and more vulnerable population. Methods: The study integrates observational data collected through a survey using a probabilistic, overlapping panel design, and data from clinical, epidemiological, demographic, and environmental registries. The data will be analysed using advanced statistical, sampling, and machine learning techniques. The study is based on several measurements obtained from three random samples of the Andalusian (Spain) population: general population aged 16 years and over, residents of disadvantaged areas, and people over the age of 55. Discussion: Given the current characteristics of this pandemic and its future repercussions, this project will generate relevant information on a regular basis, commencing from the beginning of the State of Alarm. It will also establish institutional alliances of great social value, explore and apply powerful and novel methodologies, and produce large, integrated, high-quality and open-access databases. The information described here will be vital for health systems in order to design tailor-made interventions aimed at improving the health care, health, and quality of life of the populations most affected by the COVID-19 pandemic.
Nonlinear Relationship Between HbA1c and Coronary Artery Calcium Score Progression: A Secondary Analysis Based on a Retrospective Cohort Study
Authors: Yu Jing , Gao Bo 
Published: May 2021

Abstract
ObjectiveSome previous studies was to clarify the correlation between glycated hemoglobin(HbA1c) and coronary heart disease (CHD) and the evidence regarding the correlation was still debated. However, there are fewer scientific dissertations about the correlation between HbA1c and coronary artery calcium score progression. Consequently, the present study was undertaken to explore the link of HbA1c on coronary artery calcium score progression in South Korea.MethodsThis study is a secondary analysis based on a retrospective cohort study. 8151 participants received a health check-up program at the Health Promotion Center of the Samsung Medical Center in Seoul, South Korea, from March 1, 2003 to December 31, 2013. We then used Cox proportional-hazards regression model to evaluate the independent relationship between HbA1c and coronary artery calcium score progression.ResultsAfter adjusting potential confounders (age, sex, BMI, height, weight, SBP, DBP, TC, LDL-C, HDL-C, triglycerides,smoking status, alcohol consumption, reflux esophagitis status, hypertension, diabetes, dyslipidemia, ischemic heart disease and cerebrovascular disease), non-linear relationship was detected between HbA1c and coronary artery calcium score progression, whose point was 5.8%. The effect sizes and the confidence intervals on the left and right sides of inflection point were 2.05 (1.85 to 2.27) and 1.04 (0.99 to 1.10) , respectively. Conclusion﻿The relationship between HbA1c and coronary artery calcium score progression is non-linear. HbA1c is positively related with coronary artery calcium score progression when HbA1c was less than 5.8%.
Decade-long remissions of leukemia sustained by the persistence of activated CD4+ CAR T-cells
Authors: Melenhorst Jan , Chen Gregory , Wang Meng , Porter David , Gao Peng , Bandyopadhyay Shovik , Pruteanu-Malinici Iulian , Nobles Christopher , Maji Sayantan , Frey Noelle , Gill Saar , Tian Lifeng , Kulikovskaya Irina , Gupta Minnal , Davis Megan , Fraietta Joseph , Brogdon Jennifer L. , Young Regina , Ambrose David , Chew Anne , Levine Bruce , Siegel Donald , Alanio Cécile , Wherry E. , Bushman Frederic , Lacey Simon , Tan Kai , June Carl 
Published: May 2021

Abstract
The adoptive transfer of T lymphocytes reprogrammed to target tumor cells has demonstrated significant potential in various malignancies. However, little is known about the long-term potential and the clonal stability of the infused cells. Here, we studied the longest persisting CD19 redirected chimeric antigen receptor (CAR) T cells to date in two chronic lymphocytic leukemia (CLL) patients who achieved a complete remission in 2010. CAR T-cells were still detectable up to 10+ years post-infusion, with sustained remission in both patients. Surprisingly, a prominent, highly activated CD4+ population developed in both patients during the years post-infusion, dominating the CAR T-cell population at the late time points. This transition was reflected in the stabilization of the clonal make-up of CAR T-cells with a repertoire dominated by few clones. Single cell multi-omics profiling via Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq) with TCR sequencing of CAR T-cells obtained 9.3 years post-infusion demonstrated that these long-persisting CD4+ CAR T-cells exhibited cytotoxic characteristics along with strong evidence of ongoing functional activation and proliferation. Our data provide novel insight into the CAR T-cell characteristics associated with long-term remission in leukemia.
Lipoprotein Lipase Hydrolysis Products Induce Pro-Inflammatory Cytokine Expression in Triple-Negative Breast Cancer Cells
Authors: Tobin Alexandria J. , Noel Nicholas P. , Christian Sherri L. , Brown Robert J. 
Published: May 2021

Abstract
Objectives: Lipoprotein lipase (LPL) is an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lipoproteins. LPL is highly expressed in adipose tissue and expressed in some breast cancer cell lines. Hydrolysis products generated by LPL can be used by cells as components of the cell membrane, as an energy supply, or as signaling molecules. Therefore, LPL on or around cancer cells may contribute to breast cancer growth and progression. We hypothesized that hydrolysis products generated by LPL from total lipoproteins can promote pro-inflammatory cytokine secretion from breast cancer cells and/or affect viability. Results: Using cytokine arrays, we found that the secretion of seven cytokines was increased by MDA-MB-231 cells treated with lipoprotein hydrolysis products. An increased secretion of TNF-α and IL-6 was also seen by MDA-MB-468 cells, and an increase in IL-4 secretion was seen by MDA-MB-468 and SKBR3 cells. In contrast, MCF-7 cells showed a decreased secretion of only two cytokines. The changes to cytokine secretion profiles by the breast cancer cell types, including by non-cancerous MCF-10a breast cells, were independent of increased cell metabolic activity. Overall, these results provide information on how lipoprotein hydrolysis products within the tumor microenvironment might affect breast cancer cell viability and tumor progression.
Bioinformatic Analysis of Transcriptional Regulation by Nur77 in Central Nervous System and Immune System
Authors: Costa Montserrat Olivares , Faunes Fernando , Andrés María Estela 
Published: May 2021

Abstract
ObjectiveThe objectives of this work were to find genes regulated by Nur77 in neurons and to evaluate the possible common role of this transcription factor in neurons and lymphatic cells using published experimentally generated databases of ChIP-Seq and a microarray. We also characterized Nur77 binding throughout the genome. Results﻿We identified 113 Nur77 target genes in neuronal stem cells and 116 in neuronal cells. Cell adhesion and anchoring processes emerged as regulated by Nur77 in neurons and lymphatic cells. We found 9 common genes regulated by Nur77. Finally, we described a significant distribution of Nur77 binding sites in strong enhancers and active promoters. This work is a first step to understand the role of Nur77 and its common targets in neurons and immune cells.
Effects of artificial cycles with and without gonadotropin-releasing hormone agonist pretreatment on frozen embryo transfer outcomes in patients with adenomyosis
Authors: Li Muzi , Xu Lihong , Zhao Heng , Yan Lei , Du Yanbo 
Published: May 2021

Abstract
Gonadotropin-releasing hormone agonist(GnRH-a) is generally added to improve pregnancy outcomes of adenomyosis based hormone replacement therapy cycle. Our objective in this study is to investigate whether adding GnRH-a can obtain better pregnancy outcomes. In this retrospective analysis, a total of 341 patients with adenomyosis complicated in vitro fertilization-embryo transfer(IVF-ET) of the frozen embryo transfer (FET). The control group was only treated by hormone replacement therapy cycles to prepare emdometrium, and the study group was added GnRH-a before using hormone to adjust menstruation period. Based on the similar baseline values and embryological data, there was no significantly difference about their clinical pregnancy rates (40.63% vs 42.54%, P=0.72) and live birth rates (23.75% vs 23.75%, P=0.74) between the control group and the study group. Other secondary outcomes including clinical miscarriage rates, ectopic pregnancy rates, preterm pregnancy rates and term pregnancy rates did not show significant difference between the two groups. Compared with using hormone replacement therapy cycle alone, GnRH-a down-regulation based on hormone replacement therapy cycle may not increase the rates of clinical pregnancy and live birth rates in IVF-ET of FET for infertile patients with adenomyosis.
Successful Surgical Treatment of Traumatic Macular Hole With Total Rhegmatogenous Retinal Detachment in a Child- a Case Report
Authors: Cisiecki Sławomir , Boninska Karolina , Bednarski Maciej 
Published: May 2021

Abstract
Background: To investigate the surgical results and morphologic characteristics of pediatric traumatic macular hole with total rhegmatogenous retinal detachment.Case presentation: The 4-year-old-male patient underwent scleral buckling surgery combined with vitrectomy with the inverted ILM flap technique and silicon oil tamponade for five months. Complete ophthalmic examination was performed preoperatively and 7 days, 1, 3, 6, 9 and 12 months after surgery. Successful retinal reattachment was achieved and the macular hole was successfully closed. Visual acuity improved from Hand Motion at the initial visit to 20/80 (0.6 LogMAR) postoperatively.Conclusions: Scleral buckling surgery and vitrectomy with the inverted ILM flap technique appeared to give effective anatomical and functional results in this case.
Assessment of Multiple Water-Related Hazards under Changing Climate in An Urbanized Sub-Region of Yom River Basin, Thailand
Authors: Nitivattananon Vilas , Chao-amonphat Sutinee , Huang Jinliang 
Published: May 2021

Abstract
In the Chao Phraya River Basin, Thailand, water-related hazards, particularly river floods, flash floods and droughts, are increasingly causing damages to the local society, economy and environment due to changing climate and urbanization. As its impact, identification of key factors influencing the occurrence and severity of multiple water-related hazards at different temporal and spatial scales is therefore essential to further development of solutions for hazard reduction. The study identified through the analytic hierarchy process several key influence factors to water-related hazards, including precipitation, discharges, natural and green surface areas, and water storage, in the Upper Chao Phraya River basin, Thailand. These factors were then used for the assessments of individual and multiple hazards, as well as potential interventions for hazard reduction. A combination of several research methods was adopted for the hazard assessments, including simulation modelling, multi-criteria decision analysis, and spatial analysis based on both primary and secondary data sources. Eventually, spatial distribution of hazard levels at regional and local scales was mapped out to inform water-related multiple hazards and the potential of selected nature-based solutions to such hazards based on the data of specific years. It was found that increasing vegetation areas and having nature-based reservoirs can effectively improve water management in both wet and dry seasons, contributing to hazard reduction. It is expected that the assessments performed in this study can contribute to awareness-raising of water-related hazards and eventually enhance the preparedness of risk management in the studied areas.