bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frozen n-Tetradecane Investigated by Cryo-Atom Probe Tomography
Authors: Meng Kuan , Schwarz Tim M , Weikum Eric M , Stender Patrick , Schmitz Guido 
Published: September 2021

Abstract
Atom probe tomography (APT) has been established in the microscopic chemical and spatial analysis of metallic or semiconductors nanostructures. In recent years, and especially with the development of a transfer shuttle system and adapted preparation protocols, the field of frozen liquids has been opened up. Still, very limited knowledge is available about the evaporation and fragmentation behavior of frozen liquids in APT. In this work, efforts were made to extend the method toward organic and biological soft matter, which are mostly built from hydrocarbon chains, the evaporation and fragmentation behavior of simple alkane chains (n-tetradecanes). Tetradecane shows a very complex evaporation behavior whereby peaks of C1–C15 can be observed. Based on multihit events and the representation of these in correlation plots, more detailed information about the evaporation behavior and the decay of molecules into smaller fragments in the region near the tip can be studied. A variety of different dissociation tracks of larger molecules in their excited state and their subsequent decay in low-field regions, on the way to the detector, could be observed and the dissociation zone in the low-field region was calculated.
Blackface Minstrelsy and the Reception of Elizabeth Taylor Greenfield
Authors: Chybowski Julia J. 
Published: September 2021

AbstractThis article explores blackface minstrelsy in the context of Elizabeth Taylor Greenfield's singing career of the 1850s–1870s. Although Greenfield performed a version of African American musicality that was distinct from minstrel caricatures, minstrelsy nonetheless impacted her reception. The ubiquity of minstrel tropes greatly influenced audience perceptions of Greenfield's creative and powerful transgressions of expected race and gender roles, as well as the alignment of race with mid-nineteenth-century notions of social class. Minstrel caricatures and stereotypes appeared in both praise and ridicule of Greenfield's performances from her debut onward, and after successful US and transatlantic tours established her notoriety, minstrel companies actually began staging parody versions of Greenfield, using her sobriquet, “Black Swan.” These “Black Swan” acts are evidence that Greenfield's achievements were perceived as threats to established social hierarchies.
Queers of Steel: Camp in John Williams's Superman (1978) and Jerry Goldsmith's Supergirl (1984)
Authors: Edgar Grace 
Published: September 2021

AbstractWhen Richard Donner's blockbuster Superman arrived in theaters in 1978, critics praised the filmmakers for avoiding camp in their adaptation of the comic book, comparing the film positively to the Batman television series (1966–68) of the previous decade. Several sequels later, critics attributed the series’ diminishing financial returns to its growing investment in camp, an investment that peaked in Jeannot Szwarc's Supergirl spinoff (1984), a critical and commercial misfire. Drawing on the work of camp theorists Susan Sontag, Moe Meyer, and Andrew Ross, I argue that critics have misrepresented the place of camp within the franchise, a misunderstanding stemming from their neglect of the film scores. There are two different yet overlapping forms of camp operating within these films. In their reviews, critics expressed disdain for pop camp, a highly legible form of camp, characterized by overblown performances, that the Batman show popularized in the 1960s. My analyses of Superman and Supergirl center on a subtler camp aesthetic present from the very start that critics celebrated: a playful mismatching of gender signifiers and an undercutting of heteronormative romantic relationships that I refer to as queer camp. I offer queer readings of John Williams's and Jerry Goldsmith's leitmotivic scores for Superman and Supergirl—supposedly the least campy and the campiest films in the franchise—to bring into focus the overlooked political work the series accomplishes. In a bold reclamation of the comic books’ suppressed queer legacy, the films’ queer camp aesthetic subverts the heteronormativity of the protagonists to construct Superman and Supergirl as queers of steel.
Three‐Dimensional Subsurface Flow Path Controls on Flow Permanence
Authors: Dohman J. M. , Godsey S. E. , Hale R. L. 
Published: September 2021

AbstractIntermittent streams currently constitute >50% of the global river network, and the number of intermittent streams is expected to increase due to changes in land use and climate. Surface flows are known to expand and contract within the headwater channel network due to changes in the water table driven by climate, often changing seasonally. However, the underlying causes of disconnections and reconnections throughout the stream network remain poorly understood and may reflect subsurface flow capacity. We assess how 3D subsurface flowpaths control local surface flows at Gibson Jack Creek in the Rocky Mountains, Idaho, USA. Water table dynamics, hydraulic gradients, and hyporheic exchange were monitored along a 200‐m section of the stream throughout the seasonal recession in WY2018. Shallow lateral hillslope‐riparian‐stream connectivity was more frequent in transects spanning perennially flowing stream reaches than intermittent reaches. During low‐flow periods, larger losing vertical hydraulic gradients were observed in paired piezometers in intermittent reaches than in adjacent perennial reaches. Contrary to dominant conceptual models, longitudinal measurements of hydrologic exchange in both intermittent and perennial reaches were seasonally variable except for one perennial reach that showed consistent significant gains. Observed drying dynamics, as well as subsurface pathways, were highly variable even over short distances (30 m). Flow probability and subsurface flow capacity at upstream locations can be assessed with an outlet hydrograph and upstream flow measurements. Accurate characterization of subsurface storage, discharge, and connection is critical to understanding the drivers of drying cycles in intermittent streams and their likely responses to future change.
Upslope Failure Mechanisms and Criteria in Submarine Landslides: Shear Band Propagation, Slab Failure and Retrogression
Authors: Zhang Wangcheng , Klein Boaz , Randolph Mark F. , Puzrin Alexander M. 
Published: September 2021

AbstractThe volume of a submarine landslide is likely to be amplified by shear band propagation along a basal surface or spreading failure extension in the sliding layer. Although the mechanism of translational submarine landslides has been understood using the interpretation of shear band propagation, assessment of the limits of failure extension, either through shear band propagation or retrogressive spreading, remains a challenge due to the diversity of post failure mechanisms. The paper aims to explore all post failure possibilities and quantify failure initiation and extension accordingly, focusing on the upslope conditions. The different failure mechanisms are explored with the aid of the large deformation finite element modeling. Original criteria for scarp position, shear band length at slab failure, retrogression conditions and ultimate spreading limits are proposed, following the process zone approach considering force equilibrium of the sliding mass. Simplified analytical predictions are shown to agree well with the numerical observations. The findings and criteria presented in the study are expected to help assess the ultimate scale of upslope failure in submarine landslides, which is important in determining geohazard zonation of submarine landslides.
Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer
Authors: Ma Zijian , Wei Ke , Yang Fengming , Guo Zizhang , Pan Chunfeng , He Yaozhou , Wang Jun , Li Zhihua , Chen Liang , Chen YiJiang , Xia Yang 
Published: September 2021

AbstractMetastasis is the main cause of death in patients with advanced lung cancer. The exosomes released by cancer cells create tumor microenvironment, and then accelerate tumor metastasis. Cancer-derived exosomes are considered to be the main driving force for metastasis niche formation at foreign sites, but the mechanism in Non-small cell lung carcinoma (NSCLC) is unclear. In metastatic NSCLC patients, the expression level of miR-3157-3p in circulating exosomes was significantly higher than that of non-metastatic NSCLC patients. Here, we found that miR-3157-3p can be transferred from NSCLC cells to vascular endothelial cells through exosomes. Our work indicates that exosome miR-3157-3p is involved in the formation of pre-metastatic niche formation before tumor metastasis and may be used as a blood-based biomarker for NSCLC metastasis. Exosome miR-3157-3p has regulated the expression of VEGF/MMP2/MMP9 and occludin in endothelial cells by targeting TIMP/KLF2, thereby promoted angiogenesis and increased vascular permeability. In addition, exosome miR-3157-3p promoted the metastasis of NSCLC in vivo.
CISD3 inhibition drives cystine-deprivation induced ferroptosis
Authors: Li Yanchun , Wang Xin , Huang Zhihui , Zhou Yi , Xia Jun , Hu Wanye , Wang Xu , Du Jing , Tong Xiangmin , Wang Ying 
Published: September 2021

AbstractFerroptosis, a new form of programmed cell death, not only promotes the pathological process of various human diseases, but also regulates cancer progression. Current perspectives on the underlying mechanisms remain largely unknown. Herein, we report a member of the NEET protein family, CISD3, exerts a regulatory role in cancer progression and ferroptosis both in vivo and in vitro. Pan-cancer analysis from TCGA reveals that expression of CISD3 is generally elevated in various human cancers which are consequently associated with a higher hazard ratio and poorer overall survival. Moreover, knockdown of CISD3 significantly accelerates lipid peroxidation and accentuates free iron accumulation triggered by Xc– inhibition or cystine-deprivation, thus causing ferroptotic cell death. Conversely, ectopic expression of the shRNA-resistant form of CISD3 (CISD3res) efficiently ameliorates the ferroptotic cell death. Mechanistically, CISD3 depletion presents a metabolic reprogramming toward glutaminolysis, which is required for the fuel of mitochondrial oxidative phosphorylation. Both the inhibitors of glutaminolysis and the ETC process were capable of blocking the lipid peroxidation and ferroptotic cell death in the shCISD3 cells. Besides, genetic and pharmacological activation of mitophagy can rescue the CISD3 knockdown-induced ferroptosis by eliminating the damaged mitochondria. Noteworthily, GPX4 acts downstream of CISD3 mediated ferroptosis, which fails to reverse the homeostasis of mitochondria. Collectively, the present work provides novel insights into the regulatory role of CISD3 in ferroptotic cell death and presents a potential target for advanced antitumor activity through ferroptosis.
Neutralizing antibodies for the prevention and treatment of COVID-19
Authors: Du Lanying , Yang Yang , Zhang Xiujuan 
Published: September 2021

AbstractSevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2) initiates the infection process by binding to the viral cellular receptor angiotensin-converting enzyme 2 through the receptor-binding domain (RBD) in the S1 subunit of the viral spike (S) protein. This event is followed by virus–cell membrane fusion mediated by the S2 subunit, which allows virus entry into the host cell. Therefore, the SARS-CoV-2 S protein is a key therapeutic target, and prevention and treatment of coronavirus disease 2019 (COVID-19) have focused on the development of neutralizing monoclonal antibodies (nAbs) that target this protein. In this review, we summarize the nAbs targeting SARS-CoV-2 proteins that have been developed to date, with a focus on the N-terminal domain and RBD of the S protein. We also describe the roles that binding affinity, neutralizing activity, and protection provided by these nAbs play in the prevention and treatment of COVID-19 and discuss the potential to improve nAb efficiency against multiple SARS-CoV-2 variants. This review provides important information for the development of effective nAbs with broad-spectrum activity against current and future SARS-CoV-2 strains.
Towards access for all: 1st Working Group Report for the Global Gene Therapy Initiative (GGTI)
Authors: Adair Jennifer E. , Androski Lindsay , Bayigga Lois , Bazira Deus , Brandon Eugene , Dee Lynda , Deeks Steven , Draz Mohamed , Dubé Karine , Dybul Mark , Gurkan Umut , Harlow Evelyn , Kityo Cissy , Louella Michael , Malik Punam , Mathews Vikram , McKemey Adrian , Mugerwa Henry , Muyanja Daniel , Olayiwola Olabimpe , Orentas Rimas J. , Popovski Alex , Sheehy Jeff , Ssali Francis , Nsubuga Moses Supercharger , Tisdale John F. , Verhoeyen Els , Dropulić Boro 
Published: September 2021

AbstractThe gene and cell therapy field saw its first approved treatments in Europe in 2012 and the United States in 2017 and is projected to be at least a $10B USD industry by 2025. Despite this success, a massive gap exists between the companies, clinics, and researchers developing these therapeutic approaches, and their availability to the patients who need them. The unacceptable reality is a geographic exclusion of low-and middle-income countries (LMIC) in gene therapy development and ultimately the provision of gene therapies to patients in LMIC. This is particularly relevant for gene therapies to treat human immunodeficiency virus infection and hemoglobinopathies, global health crises impacting tens of millions of people primarily located in LMIC. Bridging this divide will require research, clinical and regulatory infrastructural development, capacity-building, training, an approval pathway and community adoption for success and sustainable affordability. In 2020, the Global Gene Therapy Initiative was formed to tackle the barriers to LMIC inclusion in gene therapy development. This working group includes diverse stakeholders from all sectors and has set a goal of introducing two gene therapy Phase I clinical trials in two LMIC, Uganda and India, by 2024. Here we report on progress to date for this initiative.
Source analysis of heavy metal pollution in agricultural soil irrigated with sewage in Wuqing, Tianjin
Authors: Wang Jingran , Yu Danyang , Wang Yanhong , Du Xueli , Li Guochen , Li Bo , Zhao Yujie , Wei Yinghui , Xu Shuang 
Published: September 2021

AbstractIn this study, the contents of heavy metals and Cd and Pb isotope ratios of agricultural soil and potential source samples collected from farmland receiving sewage irrigation in Wuqing District, Tianjin, China were determined. Multiple methods were used for source analysis, including positive matrix factorization (PMF), correlation analysis, principal component analysis (PCA), and the Cd and Pb isotope ratio method. The results showed that agricultural soil was slightly contaminated by heavy metals in the research area, with relatively higher Cd and Pb accumulation levels compared to those of other heavy metals. Four types of pollution sources, including the soil parent material sources, industrial emission sources, agricultural practice sources, and mixed sources of sewage irrigation and transportation were apportioned and quantified by PMF, combined with the results of PCA and correlation analysis. The contribution rates quantified by the Cd and Pb isotope ratio method were similar, suggesting that no single source dominates Pb and Cd pollution. The contribution rates of Pb analyzed by the isotope ratio method were almost identical to those of the PMF model, indicating the rationality of the PMF result. Our results suggested that correlation analysis and PCA should be utilized to provide information for obtaining reasonable results and defining source categories for PMF, whereas the isotope ratio method should be applied to verify the accuracy of source contributions analyzed by PMF.
Retinal blood flow reversal quantitatively monitored in out-of-plane vessels with laser Doppler holography
Authors: Puyo Léo , Paques Michel , Atlan Michael 
Published: September 2021

AbstractLaser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry that reveals the local direction of blood flow with respect to the optical axis in out-of-plane vessels. This directional information is overlaid on standard grayscale blood flow images to depict flow moving towards the camera in red and flow moving away from the camera in blue, as in ultrasound color Doppler imaging. We show that thanks to the strong contribution of backscattering to the Doppler spectrum in out-of-plane vessels, the local axial direction of blood flow can be revealed with a high temporal resolution, which enables us to evidence pathological blood flow reversals. We also demonstrate the use of optical Doppler spectrograms to quantitatively monitor retinal blood flow reversals.
Interactions of anti-COVID-19 drug candidates with hepatic transporters may cause liver toxicity and affect pharmacokinetics
Authors: Ambrus Csilla , Bakos Éva , Sarkadi Balázs , Özvegy-Laczka Csilla , Telbisz Ágnes 
Published: September 2021

AbstractTransporters in the human liver play a major role in the clearance of endo- and xenobiotics. Apical (canalicular) transporters extrude compounds to the bile, while basolateral hepatocyte transporters promote the uptake of, or expel, various compounds from/into the venous blood stream. In the present work we have examined the in vitro interactions of some key repurposed drugs advocated to treat COVID-19 (lopinavir, ritonavir, ivermectin, remdesivir and favipiravir), with the key drug transporters of hepatocytes. These transporters included ABCB11/BSEP, ABCC2/MRP2, and SLC47A1/MATE1 in the canalicular membrane, as well as ABCC3/MRP3, ABCC4/MRP4, SLC22A1/OCT1, SLCO1B1/OATP1B1, SLCO1B3/OATP1B3, and SLC10A1/NTCP, residing in the basolateral membrane. Lopinavir and ritonavir in low micromolar concentrations inhibited BSEP and MATE1 exporters, as well as OATP1B1/1B3 uptake transporters. Ritonavir had a similar inhibitory pattern, also inhibiting OCT1. Remdesivir strongly inhibited MRP4, OATP1B1/1B3, MATE1 and OCT1. Favipiravir had no significant effect on any of these transporters. Since both general drug metabolism and drug-induced liver toxicity are strongly dependent on the functioning of these transporters, the various interactions reported here may have important clinical relevance in the drug treatment of this viral disease and the existing co-morbidities.
25,000 Years long seismic cycle in a slow deforming continental region of Mongolia
Authors: Bollinger Laurent , Klinger Yann , Forman Steven L. , Chimed Odonbaatar , Bayasgalan Amgalan , Munkhuu Ulziibat , Davaasuren Ganzorig , Dolgorsuren Tulga , Enkhee Bayarsaikhan , Sodnomsambuu Demberel 
Published: September 2021

AbstractThe spatial distribution of large earthquakes in slowly deforming continental regions (SDCR) is poorly documented and, thus, has often been deemed to be random. Unlike in high strain regions, where seismic activity concentrates along major active faults, earthquakes in SDCR may seem to occur more erratically in space and time. This questions classical fault behavior models, posing paramount issues for seismic hazard assessment. Here, we investigate the M7, 1967, Mogod earthquake in Mongolia, a region recognized as a SDCR. Despite the absence of visible cumulative deformation at the ground surface, we found evidence for at least 3 surface rupturing earthquakes during the last 50,000 years, associated with a slip-rate of 0.06 ± 0.01 mm/year. These results show that in SDCR, like in faster deforming regions, deformation localizes on specific structures. However, the excessive length of return time for large earthquakes along these structures makes it more difficult to recognize earthquake series, and could conversely lead to the misconception that in SDCR earthquakes would be randomly located. Thus, our result emphasizes the need for systematic appraisal of the potential seismogenic structures in SDCR in order to lower the uncertainties associated with the seismogenic sources in seismic hazard models.
Seeded growth of ultrathin gold nanoshells using polymer additives and microwave radiation
Authors: Lermusiaux Laurent , Plissonneau Marie , Bertry Laure , Drisko Glenna L. , Buissette Valérie , Le Mercier Thierry , Duguet Etienne , Tréguer-Delapierre Mona 
Published: September 2021

AbstractNanoshells made of a silica core and a gold shell possess an optical response that is sensitive to nanometer-scale variations in shell thickness. The exponential red shift of the plasmon resonance with decreasing shell thickness makes ultrathin nanoshells (less than 10 nm) particularly interesting for broad and tuneable ranges of optical properties. Nanoshells are generally synthesised by coating gold onto seed-covered silica particles, producing continuous shells with a lower limit of 15 nm, due to an inhomogeneous droplet formation on the silica surface during the seed regrowth. In this paper, we investigate the effects of three variations of the synthesis protocol to favour ultrathin nanoshells: seed density, polymer additives and microwave treatment. We first maximised gold seed density around the silica core, but surprisingly its effect is limited. However, we found that the addition of polyvinylpyrrolidone during the shell synthesis leads to higher homogeneity and a thinner shell and that a post-synthetic thermal treatment using microwaves can further smooth the particle surface. This study brings new insights into the synthesis of metallic nanoshells, pushing the limits of ultrathin shell synthesis.
Polyethyleneimine impregnated alginate capsule as a high capacity sorbent for the recovery of monovalent and trivalent gold
Authors: Dangi Yub Raj , Bediako John Kwame , Lin Xiaoyu , Choi Jong-Won , Lim Che-Ryong , Song Myung-Hee , Han Minhee , Yun Yeoung-Sang 
Published: September 2021

AbstractFor the first time, a polyethyleneimine-impregnated alginate capsule (PEIIAC) with a high adsorption capacity is developed for the recovery of monovalent and trivalent gold from an acidic solution. The strategy results in a new type of adsorbent, polyethyleneimine impregnated alginate capsule (PEIIAC) with a core–shell structure having a large number of amine groups as cationic binding site, facilitating maximum uptake of anionic auric chloride. The maximum uptake of PEIIAC was 3078 and 929 mg/g for Au (III) and Au (I), respectively, are recordable compared to other reported adsorbents to date. The as-prepared material was executed to check the sorption efficacy for Au (III) and Au (I) in the pH range of 1–12. With an increment in pH, the uptake capacity for Au (III) increased, while the uptake capacity for Au (I) decreased. The FTIR, XRD, and XPS studies revealed that the gold adsorption mechanism includes ionic interactions and reduction, wherein the amine, hydroxyl, and carboxyl groups are involved. The capsule showed a higher adsorption efficiency than other reported sorbents, making the material applicable in acidic solutions for the recovery of Au (I) and Au (III).