bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polycytosine RNA-Binding Protein 1 Regulates Osteoblast Function via a Ferroptosis Pathway in Type 2 Diabetic Osteoporosis
Authors: Ma Hongdong , Jiang yueming , Li Haitian , Yang Maowei 
Published: October 2021

Abstract
In recent years, type 2 diabetic osteoporosis has become a research hotspot for the complications of diabetes, but the specific mechanism of its occurrence and development remain unknown. Ferroptosis caused by iron overload is considered to be one of the important cause of type 2 diabetic osteoporosis. Polycytosine RNA-binding protein 1 (PCBP1), an iron ion chaperone, was considered as a protector of ferroptosis. The present study aimed to investigate the existence of ferroptosis and specific role of PCBP1 in the development of type 2 diabetes. Firstly, a Cell Counting Kit-8 assay was used to detect the changes of osteoblast viability under the influence of high glucose (HG) and/or ferroptosis inhibitor given at different concentrations and at different times. In addition, the morphological changes of mitochondria in osteoblasts under high glucose were examined via transmission electron microscopy, and the expression levels of PCBP1, ferritin and the ferroptosis-related protein glutathione peroxidase 4 (GPX4) under the action of high glucose were detected via western blotting. Furthermore, a lentivirus was used to silence and overexpress PCBP1. Western blotting was used to detect the expression of levels of the osteoblast functional proteins osteoprotegerin (OPG) and osteocalcin (OCN), while flow cytometry was used to detect the changes of reactive oxygen species (ROS) levels in each group. Under the action of high glucose, the viability of osteoblasts was significantly decreased and the number of mitochondria undergoing atrophy was significantly increased, PCBP1 and ferritin expression levels were increased and GPX4 expression was decreased. Western blotting results demonstrated that infection of the lentivirus overexpressing PCBP1, increased the expression levels of ferritin, GPX4, OPG and OCN, compared with the high glucose group. The flow cytometry results identified a reduction in ROS, and an opposite result was obtained after silencing PCBP1. In conclusion, it was suggested that PCBP1 may protect osteoblasts and reduce the harm caused by ferroptosis by promoting ferritin expression under a high glucose environment. Moreover, it was indicated that PCBP1 may be a potential therapeutic target for treating type 2 diabetic osteoporosis.
Naringin Administration Mitigates Oxidative Stress, Anaemia and Hypertension in Lead Acetate-Induced Cardiorenal Dysfunction in Cockerel Chicks
Authors: Ebirim Chinomso Gift , Esan Oluwaseun , Adetona Moses Olusola , Oyagbemi Ademola Adetokunbo , Omobowale Temidayo Olutayo , Oladele Omolade Abodunrin , Adedapo Adeolu Alex , Oguntibeju Olufemi Omoniyi , Yakubu Momoh Audu 
Published: October 2021

Abstract
Lead is one of the major pollutants in the world which is deleterious to both animals and humans. It is found in every aspect of the environment such as the air, water and soil. This pollutant affect both wild and domestic birds. Naringin is a flavonoid that has been found to have medicinal properties mostly because of its antioxidant and metal chelating effects. This study was carried out to investigate the protective effect of naringin against lead-induced anaemia, cardio and nephrotoxicity, oxidative stress, and hypertension. Thirty -six cockerel chicks were used for this study, and randomly grouped into six chicks per group; Group A served as the control, Group B received Pb only (300 ppm), Group C (Pb and naringin; 80 mg/kg), Group D (Pb and naringin; 160 mg/kg), Group E (naringin 80 mg/kg) and Group F (naringin 160 mg/kg), respectively for eight weeks. Lead (Pb) was administered via drinking water while Naringin was administered via oral gavage. Lead acetate intoxication precipitated anaemia as indicated by significant reductions in the values of PCV, RBC, and Hb concentration in lead-treated chicks when compared with the controls. Also, lead administration induced hypertension together with increased oxidative stress, depletion of the antioxidant defense system, reduced nitric oxide production and increase in high blood pressure. Immunohistochemistry indicated high expressions of cardiac troponin, renal angiotensin converting enzymes, and renal neutrophil gelatinase associated lipocalin. Treatment with naringin corrected anemia, reduced oxidative stress, improved antioxidant system, reduced high blood pressure, and offered protection against lead acetate-induced cardio-renal dysfunction in cockerel chicks.
A Novel Immune and Inflammatory Related Gene Signature in Hepatocellular Carcinoma
Authors: Xu Yunji , Huang Guo , Li Wenbing 
Published: October 2021

Abstract
Background: The prognosis of hepatocellular carcinoma (HCC) is closely related to immunity and inflammation, but the value of using immune and inflammation-related genes as predicting the prognosis of HCC requires further research.Methods: The Hepatocellular Carcinomar mRNA data was downloaded in the TCGA and ICGC database. The R package "limma" was used to analyze the differential expression of genes (DEGs) irelated to immune and inflammatory .Univariate Cox analysis screen for immune and inflammation related genes with prognostic value, then construction and verification of the prognostic model in Hepatocellular Carcinomar. The correlation between risk score with tumor immune immersion and immune cell function was assessed through tumor microensure and immune response analysis. NCI-60 cell line to explore the relationship between prognostic gene expression and drug sensitivity.Results: We evaluated 8 immune and inflammatory-related genes to build a prognostic risk prediction model, riskscore is an independent risk factor affecting prognosis, closely related to histological grading and clinical staging. The immune of adCs, macrophages, Tfh cells, Treg cells and Th1 cells higher in the tumor microenvironment leads to poor prognosis of liver cancer. Using data from the NCI-60 cell line, DNASE1L3 high expression may increased resistance of liver cancer cells to bovine platinum, solafinil and bovine platinum. The expression of SLC7A11 can increase the sensitivity of liver cancer to arsenic trioxide (ATO). Simultaneously constructing models and tumor microenvironment and drug resistance may provide effective and safe strategies for HCC chemotherapy and immunotherapy.Conclusion:Our study screened eight immune and inflammation-related genes play an important role in HCC tumor immunity and can be used to predict the prognosis of HCC.
Primary Tumor Surgery Improves Survival in Patients With Gastroenteropancreatic Neuroendocrine Carcinoma: A Population-Based Preliminary Study
Authors: Yan Haihao , Liu Zheng 
Published: October 2021

Abstract
Background: Chemotherapy has always been the primary treatment for gastroenteropancreatic neuroendocrine carcinomas (GEP-NECs), but the efficacy of surgical resection for GEP-NECs patients receiving chemotherapy is still controversial.Methods: Information on GEP-NECs patients from 2004 to 2015 were extracted from the Surveillance, Epidemiology, and End Results (SEER) databases. Kaplan-Meier analysis and Log-rank test were used to evaluate the difference in survival rate between the surgery and the non-surgery group. Cox proportional hazard model was used to find the prognostic factors affecting overall survival (OS) time and cancer-specific survival (CSS) time. Results: A total of 589 patients participated in our study, including 196 patients who underwent surgery and 393 patients who did not undergo surgery. Kaplan-Meier curve showed that there were significant differences in OS and CSS (p<0.001) between the surgery and the non-surgery group. Multivariate COX analysis showed that surgery was an independent protective factor for prolonging the duration of OS and CSS (p < 0.001). At the same time, through the propensity score-matched (PSM) of the original data, we also draw the same conclusion.Conclusion: Our study preliminarily shows that for some patients with GEP-NECs, surgery for primary tumors may bring survival benefits. Compared with chemotherapy alone, surgery combined with chemotherapy can significantly prolong the survival time of patients.
New-Onset Atrial Fibrillation is an Independent Predictor of Mortality in Patients With Candidemia
Authors: Xiao Zengli , Du Anqi , An Youzhong 
Published: October 2021

Abstract
Background: Candidemia, or invasive candidiasis infection, is prevalent in critically ill patients and significantly contributes to the mortality and morbidity of such patients. New-onset atrial fibrillation (NOAF) also occurs frequently in critically ill patients. However, the association between NOAF and candidemia is still uncertain. This study aims to determine whether NOAF could increase the mortality rate of critically ill patients who have candidemia.Methods: We retrospectively identified NOAF in all patients who were admitted into a non-cardiac intensive care unit (ICU) and diagnosed as candidemia from January 2011 to March 2018. These patients were divided into 3 groups (NOAF, Prior AF, No AF). Clinical information and long-term outcome were collected and compared between three groups . Risk factors for these patients’ short-term and long-term mortality were also analyzed.Results: Ninety-two patients with candidemia were included from 2011 to 2018. Among these patients, 26 (28.3%) developed NOAF during their ICU hospitalization. Patients with NOAF had lower survival rate than those who never developed AF. The multivariate logistic regression analysis indicated that stroke, anemia, Sequential Organ Failure Assessment (SOFA) score and NOAF were independent risk factors for in-hospital mortality and NOAF was also an independent risk factor for 1 year mortality. Conclusions: There was a high incidence of NOAF in patients with candidemia. In this study, we found NOAF was an independent predictor of in-hospital mortality and 1 year mortality after hospital discharge for patients with candidemia.
Feedback inhibition by GABAergic interneurons that are driven by dorsal raphe-projecting pyramidal neurons of the medial prefrontal cortex suppress feeding of adolescent female mice undergoing activity-based anorexia
Authors: Du Muzi , Santiago Adrienne , Akiz Cenk , Aoki Chiye 
Published: October 2021

Abstract
Anorexia Nervosa (AN) is characterized by voluntary food restriction, excessive exercise and mortality rate surpassing that of major depression. Activity-based anorexia (ABA) is an animal model that captures these characteristics of AN, thus having the potential to reveal the neurobiology underlying individual differences in AN vulnerability. Dorsal raphe (DR) is known to regulate feeding but its role in ABA remains unexplored. Through chemogenetic activation, we investigated the role of mPFC pyramidal neurons projecting to DR (mPFC→DR) in an animal’s decision to eat or exercise following ABA induction. Although the DREADD ligand C21 could activate 44% of the mPFC→DR neurons, this did not generate significant group mean difference in the amount of food intake, compared to control ABA mice without chemogenetic activation. However, further analysis of individual animals’ responses to C21 revealed a significant, positive correlation between food intake and mPFC→ DR neurons that co-express cFos, a marker for neuronal activity. cFos expression by GABAergic interneurons (GABA-IN) in mPFC was significantly greater than that for the control ABA mice, indicating recruitment of GABA-IN by mPFC→DR neurons. Electron microscopic immunohistochemistry (EM-ICC) revealed that GABAergic innervation is 60% greater for the PFC→DR neurons than the Layer 5 pyramidal neurons without projections to DR. Moreover, individual differences in this innervation correlated negatively with food intake specifically on the day of C21 administration. We propose that C21 activates two antagonistic pathways: 1) PFC→DR pyramidal neurons that promote food intake; and 2) GABA-IN in the mPFC that dampen food intake through feedback inhibition of mPFC→DR neurons.
Plexiform Fibromyxoma of The Stomach: A Rare Case Report And Review of Literature
Authors: Wei Xiao , Kwasi Attigah S. D. , Li Qi , Wu Hongyan , Chen Jun , Sun Qi 
Published: October 2021

Abstract
Background: Plexiform fibromyxoma (PF) is a very rare mesenchymal tumor of the stomach. Here we report one case of this unusual gastric tumor that was pathologically confirmed after endoscopic resection. Its clinical and pathologic features were observed while the relevant literature was reviewed. Case presentation: A 1.0 cm round elevated submucosal mass was discovered by gastroscopy in a 44-year-old Chinese woman due to recurrent abdominal pain. The tumor was characterized by a multinodular plexiform pattern, bland-looking oval to spindle cells, and a myxoid stroma with thin arborizing capillaries. Immunohistochemistry analysis revealed that the tumor cells were positive for smooth muscle actin (SMA) and negative for CD117, DOG-1, CD34, Desmin, progesterone receptor (PR), CD10, S100 and SOX10. A diagnosis of PF was rendered.Conclusion: Gastric PF is a benign tumor without evidence of local recurrence and distant metastasis. This case emphasizes the unique histological appearance and immunophenotype of PF to promote early diagnosis, while endoscopic resection can be used as an alternative treatment for small and superficial PF.
Maternal Fermented Diet Drives Early-Life Gut Health by Boosting Milk L-Glutamine and Gut Lactobacillus Reuteri
Authors: Wang Cheng , Wei Siyu , Jin Mingliang , Wang Yizhen 
Published: October 2021

Abstract
BackgroundKnowledge is growing on how maternal nutrition shapes neonatal health. However, the effects of maternal factors on gut health in offspring and how the maternal fermented diet (MFD) affects it remain elusive. Here, we applied in vivo and in vitro models to investigate the impacts and the underlying mechanism of the MFD on early-life gut health. ResultsThe MFD remarkably improved the performance of both sows and piglets, significantly increased the abundance of maternal gut Lactobacillus, Succiniclasticum and enhanced protein and fat metabolic pathways. α-guanidinoglutaric acid, hippuric acid, L-glutamine (GLN), and pyridoxic acid, as well as glutamine metabolism in the milk were notably improved by the MFD. In addition, the MDF significantly accelerated the maturation of the gut microbiome in early life, increased the abundance of gut Lactobacillus, and the microbial functions of amino acid-related enzymes and glucose metabolism on the weaning day. Notably, the MFD reduced the susceptibility to lipopolysaccharide (LPS)-induced colonic inflammation in offspring. Faecal microbiota transplantation (FMT) suggested that the MFD might play critical roles in early-life gut health by mediating maternal gut microbiota. Multi-omics analysis and in vivo and in vitro assays revealed maternal Lactobacillus reuteri (LR) and milk GLN were two main maternal effectors that contributed to the gut health of offspring. The properties of LR and GLN in alleviating LPS-induced colonic inflammation by inhibiting the phosphorylation of p38 MAPK and JNK were further revealed in mice and cells.ConclusionsThese findings provide the first data revealing that the MFD drives early life gut microbiota development and ameliorated the colonic inflammation by boosting milk L-glutamine and gut Lactobacillus reuteri. These fundamental data might provide references for modulating maternal nutrition to prevent early life gut inflammatory bowel disease and enhance gut health.
Team IHMC At The 2020 Cybathlon: A User-Centered Approach Towards Personal Mobility Exoskeletons
Authors: Peterson Brandon , Daniel Mark , Mani Vishnu Subra , Arnold Brooke , Craig Travis , Gines Jeremy , Gonzalez Carlos , Howell William , Shrewsbury Brandon , Bellman Matthew , Neuhaus Peter , Griffin Robert 
Published: October 2021

Abstract
Background: The past few decades have seen rapid advancements in exoskeleton technology, with a considerable shift towards applications involving users with gait pathologies. Commercial devices from ReWalk, Ekso Bionics, and Indego, mainly designed for rehabilitation purposes, have inspired the development of many research platforms aimed at extending capabilities for use as safe and effective personal mobility devices. The 2016 Cybathlon featured an impressive demonstration of exoskeletons designed to enable mobility for individuals with spinal cord injury, however, not a single team completed every task and only two completed the stairs. Major improvements were showcased at the 2020 Cybathlon, with seven of the nine teams completing a similar set of tasks. Team IHMC built upon its silver-medal success from 2016 with an upgraded device, Quix. ﻿Methods: Quix features several notable improvements including an additional powered degree of freedom for hip ab/adduction to laterally shift the device and reduce user effort while walking, custom-tailored cuffs and soft goods based on 3D body scans to optimize user comfort, and a streamlined testing pipeline for online tuning of gait parameters.Results: Team IHMC finished in fourth place behind the teams from EPFL and Angel Robotics. Although we suffered from a considerably slower flat-ground walking speed, our pilot reported marked improvements in overall effort, comfort, and ease-of-use compared to our previous device.Conclusions: Clear progress in exoskeleton development has been exhibited since the inaugural Cybathlon, with tasks involving rough terrain, stairs, and ramps now posing little threat to most of the competitors. As a result, the layout of the powered exoskeleton course will likely undergo significant modifications to further push the devices towards suitability for personal everyday use. The current tasks do not address the issue of donning and doffing, nor do they simulate a scenario similar to maneuvering a kitchen to prepare a meal, for example. An additional limitation that may be more difficult to test in a competition setting is the required upper-body effort to manipulate the device in an effective manner.
Risk Factors Associated With Mortality in Hypersensitivity Pneumonitis: A Meta-analysis
Authors: Dasgupta Sanjukta , Bhattacharya Anindita , Chowdhury Sushmita Roy , Chaudhury Koel 
Published: October 2021

Abstract
Purpose: Hypersensitivity pneumonitis (HP), an immune-mediated form of diffuse parenchymal lung disease (DPLD), is triggered by inhalation of a wide variety of allergens in susceptible individuals. Several studies suggest that the death rate associated with this disease has increased significantly in recent years. This meta-analysis investigates the significant clinico-radiological characteristics which may be appraised as potential risk factors associated with disease mortality.Methods: Extensive literature search was conducted for original articles published between January 2009 and April 2021 through PubMed, Google Scholar, EMBASE, and Cochrane Library using the keywords: “hypersensitivity pneumonitis”, “hazard ratio” and “mortality”. Results: A total of 21 independent studies related to mortality of HP subjects could be identified. The combined results of univariate and multivariate analysis suggest that older age [univariate odds ratio (OR) 1.038 (1.028-1.048); multivariate OR 1.036, (1.025-1.046)], male subjects [univariate OR 1.508, (1.240- 1.834); multivariate OR 1.396, (1.004-1.943)], honeycombing [univariate OR 1.086 (1.065- 1.108); multivariate OR 1.121 (1.070- 1.175)] and traction bronchiectasis [univariate OR 1.141 (1.092- 1.192); multivariate OR 1.107 (1.048-1.169)] are significantly associated with mortality risk of HP subjects. Further, forced vital capacity (FVC), diffusing capacity for carbon monoxide (DLco), ground glass opacity (GGO) and mosaic attenuation were associated with lower risk of all-cause mortality. Although smoking status correlated with mortality risk in these patients, the findings appeared to be insignificant. Conclusion: Individual male subjects with older age and presence of extensive fibrosis, i.e., honeycombing and traction bronchiectasis experience an increased mortality risk.
The Effects of a Group-based Intervention through Physical Activities and Diet in Young Patients with Serious Psychiatric Disorders: An Exploratory Study
Authors: Sentissi Othman , Zosso Gabrielle , Cogordon Anouck , Chilla Chiarra 
Published: October 2021

Abstract
Background and objectives: The present study aims to investigate the effect of the 4-F (Fit, Fun, Feel and Food) group-based program on physical, clinical and biological outcomes in young patients suffering from serious psychiatric disorders.Methods: To prevent weight gain and improve mental and physical health in young patients, we investigated in a naturalistic design, the effect of a group-based intervention through an exploratory study.Results: We observed that out of the 61 outpatients initially included in the program, 71% were overweight or obese. The 24 patients who completed the full program showed no significant decrease in weight or body composition. Our main findings were the significant improvement in muscular endurance as well as coordination from T0 (M=13.65, SD=±1.93) compared to T1 (M=12.49, SD= ±1.81), (t(20) = 3.072 p = <0.05) and the increase in the general mental well-being of these patients from baseline to the end of the program. According to the type of psychopathology, (F(3,10) = 4.25, p < .05), we also noticed a slight modification in eating behaviour with a tendency to decrease in the TFEQ hunger’s level.Conclusion: Despite the limitations, the findings of this exploratory study demonstrated that this program is feasible and resulted in better physical conditions with enhanced mental well-being in young patients suffering from psychiatric disorders, sedentary behaviour and unhealthy lifestyles. Further research is warranted in controlled and larger population samples to further our understanding of the effect of such interventions.
Removal of oil from produced water using electrochemical method
Authors: M Sumayya V , P.A Soloman 
Published: October 2021

Abstract
The produced water usually contains 1000 mg/litre of crude oil in dissolved, suspended and emulsion form. It is required to reduce the crude oil content to less than 40 mg/litre, to meet the latest discharge regulations. Current produced water technologies include centrifugation, compact floatation units, gravity settling, sand filtration, macroporous polymer extraction and ozonation. But their applications have certain disadvantages. This project is aimed to deal with the use of electro­chemical methods for the removal of oil from produced water and thereby reducing the environmental impact of produced water. An experimental setup for electro­coagulation, containing a stainless-steel cathode and a mild steel anode is used in the present study. In the electro­coagulation process, the coagulant is generated in situ by electrolytic oxidation of anode material, which facilitates agglomeration or coagulation of oil droplets and suspended solids and resultant separation from the aqueous phase. The response surface methodology software has been used to determine the relation between oil removal performance of the system and operating parameters such as current density, time and initial pH.