bluuebunny/crossref_metadata_2025_split huggingface dataset:
ROLE OF GSK-3 IN Wnt/β-CATENIN SIGNALING PATHWAY IN OBESITY
Authors: Kulakova A. S. , Snimshchikova I. A. , Plotnikova M. O. 
Published: October 2021

The complexity of the adipogenesis mechanism results from the impact of multiple cues, among which an important place is held by the components of the Wnt signaling pathway. The search for potential markers of the development of diseases related to obesity aroused an interest in the study of GSK-3 (glycogen synthase kinase), β-catenin. GSK-3β is an intracellular serine / threonine kinase found in the cytoplasm, nucleus, mitochondria, synthesized in all body tissues and involved in regulating metabolic processes, cell proliferation, apoptosis etc. The first of the discovered functions of GSK-3β was the regulation of glycogen synthesis. Active GSK-3β phosphorylates and thereby inhibits glycogen synthase. As a result of the insulin binding to the cell receptor via inositol-3-phosphate, protein kinase B (Akt1) is activated, which, in turn, phosphorylates and inhibits GSK-3β. In addition, GSK-3β is involved in the regulating glucose metabolism. The most important function of GSK-3β is the inhibition of the β-catenin protein. In a resting cell, GSK-3β in complex with the APC and Axin proteins binds and phosphorylates the β-catenin transcription factor, which leads to its ubiquitination and degradation. When Wnt proteins act on the cell, the Dvl protein is activated, which, by binding to GSK-3β, releases β-catenin, preventing its degradation, however, the role of GSK3α/β in the adipocyte inflammatory response has not yet been fully investigated, therefore it seems promising to study the role of GSK-3 in the Wnt/β-catenin signaling pathway in obesityThe aim of the study was to assess the activity of the components of the Wnt signaling pathway in obese patients by measuring the serum level of GSK-3 and β-catenin. There were enrolled 32 patients with progressive forms of I-III degree obesity in the absence of diabetes mellitus. The concentration of serum GSK-3α, GSK-3β, and β-catenin was measured by enzyme-linked immunoassay. Data are presented as absolute and relative (%) number of patients; arithmetic mean; medians, 1 and 3 quartiles – Ме (Q0.25-Q0.75). Obese patients contained a 7.5-fold increased serum level of GSK-3α (785 (371-1317.5) pg/ml) compared to healthy individuals 105 (102.5-110) pg/ml, (p < 0.001), paralleled with increased amount of GSK-3β, which level in obese patients was 295 (190-695) pg/ml, which is by 18.3% higher than those in healthy individuals 241 (218.75-287.5) pg/ml, p = 0.111. Amount of GSK-3 depending on the degree of obesity tended to increase, most often coupled to decreased β-catenin level which is consistent with the literature data and can be considered as a prognostic criterion for the course of pathological processes in obesity.
SUBCELLULAR LOCALIZATION OF FoxP3 TRANSCRIPTION FACTOR IN PATIENTS WITH ACUTE CORONARY SYNDROME: COMPARATIVE ANALYSIS AND PROSPECTIVE OBSERVATION
Authors: Kologrivova I. V. , Suslova Tatiana E. , Ryabov V. V. , Shtatolkina M. A. , Koshelskaya O. A. , Trubacheva O. A. , Kravchenko E. S. 
Published: October 2021

The key cellular and molecular factors being involved in the resolution of inflammation following acute myocardial infarction remain poorly understood. T-regulatory (Treg) lymphocytes are characterized by the extreme potential to regulate the strength and direction of immune responses during the myocardial injury. The functional activity of Treg-lymphocytes depends upon the transcription factor forkhead box protein P3 (FoxP3). It may be also expressed in conventional T-lymphocytes at the stage of their activation. Nuclear localization of FoxP3 is a prerequisite factor determining its ability to impact the suppressive functions of Treglymphocytes.The aim of the present study was comparative evaluation of FoxP3+T-lymphocytes frequency and counts, combined with estimation of FoxP3 subcellular localization, in patients with acute myocardial infarction and chronic coronary syndrome and examination of changes of these parameters in the short-term follow-up of patients with myocardial infarction. The study included 14 patients with chronic coronary syndrome (8 males; 6 females; 63.2±9.0 y.o.) and 5 patients with acute anterior ST-segment elevation myocardial infarction (4 males; 1 female; 61.4±11.2 y.o.) at days 1, 3 and 7 after the event. The frequency of FoxP3+ conventional and regulatory T-lymphocytes was evaluated in peripheral blood mononuclear cells together with estimation of the level of FoxP3 nuclear localization by imaging flow cytometry.Patients with infarction were characterized by the decreased counts of FoxP3+Treg-lymphocytes compared to patients with chronic coronary syndrome, and exhibited even further decrease in the counts of FoxP3+Tregcells at day 7 after infarction, while frequency of Treg and conventional T-lymphocytes did not differ significantly. The level of FoxP3 nuclear translocation was lower both in Treg and conventional T-lymphocytes in patients at day 1 post-infarction compared to patients with chronic coronary syndrome. Absolute counts of FoxP3+Tregs with nuclear FoxP3 localization remained significantly lower both at days 1 and 7 post-infarction compared to patients with chronic coronary syndrome.Thus, here we demonstrated that FoxP3 nuclear localization experiences decrease in the course of acute myocardial infarction and may serve as a more sensitive marker of changes in Treg-lymphocyte functioning than simple evaluation of their frequency.
Impact of preoperative mild cognitive impairment on cerebrovascular events and cognitive status in patients undergoing coronary artery bypass grafting: data from 5-year follow-up
Authors: Syrova I. D. , Trubnikova O. A. , Tarasova I. V. , Maleva O. V. , Semenov S. E. , Lozhkin I. S. , Barbarash O. L. 
Published: October 2021

Aim. To assess the incidence of cerebrovascular events and cognitive status in the long-term period after coronary artery bypass grafting (CABG) in patients with and without preoperative mild cognitive impairment (MCI).Material and methods. This prospective study involved 115 patients aged 45 to 69 years, who were admitted to the hospital for elective on-pump CABG. All patients underwent clinical, laboratory, ultrasound, neuroimaging and neuropsychological examination 3-5 days before and 5-7 years after CABG. Cognitive functioning was assessed using screening neuropsychological scales and neuropsychological testing with an assessment of neurodynamics, attention and short-term memory. Pre-CABG MCI was diagnosed based on the criteria by R. Petersen et al. Therefore, the patients were divided into two groups: with (n=51) and without MCI (n=64).Results. It was found that strokes and dementia during the long-term postoperative period of CABG were observed only in the group of patients with preoperative MCI (7,84%). Five-seven years after CABG, a decrease in cognitive status according to neuropsychological scales was found in all patients (p≤0,05). A decrease in neurodynamics, attention and short-term memory compared to the preoperative level was found in 47,92% of patients with preoperative MCI, without MCI — in 40,63%. Neurodynamic disorders occurred equally frequently in both groups (63,8% and 57,8% of patients, respectively). Memory impairments were more often observed in patients with MCI (55,3%) compared to those without MCI (34,4%) (OR=2,36, 95% CI, 1,09-5,12, p=0,03). According to brain multislice computed tomography in the group with preoperative MCI, the number of patients with cysts and leukoaraiosis 5-7 years after CABG was higher than in those without MCI (p≤0,05).Conclusion. In the long-term (5-7 years) postoperative period of CABG, more than 40% of patients demonstrate a decrease in cognitive status, regardless of preoperative data. At the same time, preoperative MCI is associated with adverse cerebrovascular events and dementia, accompanied by morphological brain abnormalities. Due to the revealed facts, it is necessary to optimize approaches to drug treatment and secondary prevention of cognitive decline after cardiac surgery.
Practical efficacy and safety of Konsilar D24 in patients with hypertension: data from the KONSONANS program
Authors: Konradi A. O. , Galyavich A. S. , Kashtalap V. V. , Lopatin Yu. M. , Lyamina N. P. , Nedogoda S. V. , Nedoshivin A. O. , Nechaeva G. I. , Ratova L. G. , Shaposhnik I. I. 
Published: October 2021

Aim. In practice, to evaluate the efficacy, safety and long-term adherence to therapy with a fixed-dose combination of ramipril/indapamide (Konsilar-D24) in patients with grade 1-2 hypertension (HTN) who have not achieved blood pressure (BP) control with prior therapy or have not taken antihypertensive therapy.Material and methods. This multicenter open-label observational program included 524 patients with grade 1-2 HTN who did not take antihypertensive therapy or did not reach the target BP level with mono or dual antihypertensive therapy, as well as patients shifted to Konsilar-D24 therapy no later than two weeks before the start of the program. All patients signed a written informed consent to participate in the program. The safety analysis set includes all patients who have taken at least one dose of a fixed-dose combination of ramipril/indapamide and have visited physician at least once during the program. The effectiveness analysis set included all patients in the safety population who completed the study in accordance with protocol (n=511). Clinical systolic blood pressure (SBP), diastolic BP (DBP) and heart rate were assessed at baseline, as well as at 0,5, 1, 3 and 6 months of treatment. A post hoc subgroup analysis of changes in BP and heart rate was performed depending on age, sex and baseline body mass index.Results. The fixed-dose combination of ramipril with indapamide significantly reduced SBP and DBP after 2-week treatment (-20,9±10,1 mm Hg; pConclusion. Despite the limitations inherent in observational studies, the KONSONANS program has demonstrated high efficacy and safety of fixed-dose combination of ramipril/indapamide taken once a day in hypertensive patients. Ramipril/indapamide fixed-dose combination therapy significantly improved BP control and achieved even lower individual target BP levels in the majority of hypertensive patients.
Cardiovascular status and echocardiographic changes in survivors of COVID-19 pneumonia three months after hospital discharge
Authors: Krinochkin D. V. , Yaroslavskaya E. I. , Shirokov N. E. , Gultyaeva E. P. , Krinochkina I. R. , Korovina I. O. , Mamarina A. V. , Osokina N. A. , Melnikov N. N. , Trifanova T. A. , Gorbatenko E. A. , Petelina T. I. 
Published: October 2021

Coronavirus disease 2019 (COVID-19) affects the function of all organs and systems. Today, studying the effect of COVID-19 on cardiovascular system, including on echocardiographic characteristics, is relevant.Aim. To study the prevalence of symptoms, cardiovascular disease and changes in echocardiographic data in persons after documented COVID-19 pneumonia 3 months after discharge from the hospital.Material and methods. The study included 106 patients after documented COVID-19 pneumonia. The patients underwent a comprehensive examination during hospitalization and 3 months±2 weeks after discharge from the hospital. The mean age of participants was 47±16 years (19-84 years); 49% of subjects were women.Results. Three months after hospital discharge, the symptoms persisted in 86% of examined patients. There were significant echocardiographic changes as follows: a decrease in LV end-diastolic, end-systolic and stroke volume (113,8±26,8 ml vs 93,5±29,4 ml; 37,7±13,0 ml vs 31,3±14,2 ml; 77,2±17,8 ml vs 62,2±18,7 ml, respectively, p<0,001 for all). The right ventricular anteroposterior dimension and the pulmonary trunk diameter decreased over time (26,0 [24,0-29,3] mm vs 25,0 [23,0-27,0] mm, p=0,004; 21,7±3,6 mm vs 18,7±2,5 mm, p<0,001), the same as the pulmonary artery systolic pressure, estimated by tricuspid regurgitation gradient (28,0 [25,0-32,25] mm Hg vs 21,5 [17,0-25,0] mm Hg). The right atrial volume (42,0 [37,0-50,0] m><0,001), the same as the pulmonary artery systolic pressure, estimated by tricuspid regurgitation gradient (28,0 [25,0-32,25] mm Hg vs 21,5 [17,0-25,0] mm Hg). The right atrial volume (42,0 [37,0-50,0] ml vs 31,0 [22,0-36,5] ml, p<0,001) a><0,001) and maximum width (36,1±4,6 mm vs 34,5±6,5 mm, p=0,023) decreased, while the right atrial maximum length increased (46,7±6,8 mm vs 48,6±7,1 mm, p=0,021).Conclusion. In survivors of COVID-19 pneumonia three months after hospital discharge, complaints persisted in 86% of cases. Cardiovascular diseases were detected in 52% of participants, including hypertension in 48,1% and coronary artery disease in 15,1%. Compared with in-hospital data, the echocardiographic characteristics improved, which was expressed mainly in a decrease in right heart load.
A hospital-based self-harm register in Mysore, South India: Is follow-up of survivors feasible in low and middle income countries?
Authors: Krishna Murali , Majgi Sumanth Mallikarjuna , Pradeep Kumar Sudeep , Rajendra Rajagopal , Heggere Narendra , Poole Rob , Robinson Catherine 
Published: October 2021

Background: In high-income countries, dedicated self-harm surveillance systems are regarded as a key component in suicide prevention strategies, which suggests they may be important in low- and middle-income countries where rates of suicide are higher and risk factors for self-harm are different, provided they can be shown to be feasible in those settings.Methods: We established a hospital based self-harm register in Mysore, South India. A subset of participants was followed-up after two years. Results: Of the 453 who were examined at baseline, the vast majority (80%) were from rural areas, nearly a quarter were illiterate and 65 (14%) were diagnosed with depression. Compared to men, women tended to be younger, single, from rural areas, unemployed, with lower levels of educational attainment and higher levels of disability. Of the 453, 371 (80%) were successfully contacted by cellphone at 2 years. There were no significant differences in baseline variables between those followed-up and those who were not, including sociodemographic features, rates of depression, severity of disability and severity of suicidal intent. All participants reported that psychosocial assessment offered at baseline was helpful and that they would recommend assessment to othersConclusions: Findings from this study indicate that our self-harm register was a feasible and useful resource, and that contact and follow up are acceptable and feasible.
Notodóntidos (Lepidoptera: Notodontidae) de la Colección Taxonómica Nacional de Insectos “Luis María Murillo”, Cundinamarca, Colombia
Authors: Prada Lara Liliana 
Published: October 2021

Se estudió el material de polillas de la familia Notodontidae (Lepidoptera) depositado en la Colección Taxonómica Nacional de Insectos “Luis María Murillo” (CTNI). Se encontraron 202 especímenes pertenecientes a 50 especies, 33 géneros y siete subfamilias. Dos de las especies son endémicas de Colombia y 20 se registran por primera vez para el país. La subfamilia Heterocampinae y el género Hemiceras presentaron el mayor número de especies, con 19 y 10 especies, respectivamente. Adicionalmente, se reportan seis nuevas plantas hospederas para la familia en el país.
Bayesian Long-Short Term Memory for History Matching in Reservoir Simulations
Authors: Santoso Ryan , He Xupeng , Alsinan Marwa , Kwak Hyung , Hoteit Hussein 
Published: October 2021

Abstract
History matching is critical in subsurface flow modeling. It is to align the reservoir model with the measured data. However, it remains challenging since the solution is not unique and the implementation is expensive. The traditional approach relies on trial and error, which are exhaustive and labor-intensive. In this study, we propose a new workflow utilizing Bayesian Markov Chain Monte Carlo (MCMC) to automatically and accurately perform history matching. We deliver four novelties within the workflow: 1) the use of multi-resolution low-fidelity models to guarantee high-quality matching, 2) updating the ranges of priors to assure convergence, 3) the use of Long-Short Term Memory (LSTM) network as a low-fidelity model to produce continuous time-response, and 4) the use of Bayesian optimization to obtain the optimum low-fidelity model for Bayesian MCMC runs.
We utilize the first SPE comparative model as the physical and high-fidelity model. It is a gas injection into an oil reservoir case, which is the gravity-dominated process. The coarse low-fidelity model manages to provide updated priors that increase the precision of Bayesian MCMC. The Bayesian-optimized LSTM has successfully captured the physics in the high-fidelity model. The Bayesian-LSTM MCMC produces an accurate prediction with narrow uncertainties. The posterior prediction through the high-fidelity model ensures the robustness and precision of the workflow. This approach provides an efficient and high-quality history matching for subsurface flow modeling.
Modelling Methane Extraction from Stimulated Coalbed Influenced by Multidomain and Thermal Effects
Authors: Li Wai , Liu Jishan , Zeng Jie , Leong Yee-Kwong , Elsworth Derek , Tian Jianwei 
Published: October 2021

Abstract
The process of extracting coalbed methane (CBM) is not only of significance for unconventional energy supply but also important in mine safety. The recent advance in fracking techniques, such as carbon dioxide (CO2) fracking, intensifies the complexity of stimulated coalbeds. This work focuses on developing a fully coupled multidomain model to describe and get insight into the process of CBM extraction, particularly from those compound-fractured coalbeds. A group of partial differential equations (PDEs) are derived to characterize gas transport from matrix to fractures and borehole. A stimulated coalbed is defined as an assembly of three interacting porous media: matrix, continuous fractures (CF) and radial primary hydraulic fracture (RF). Matrix and CF constitute a dual-porosity-dual-permeability system, while RF is simplified as an 1-D cracked medium. These media further form three distinct domains: non-stimulated reservoir domain (NSRD), stimulated reservoir domain (SRD) and RF. The effects of coal deformation, heat transfer, and non-thermal sorption are coupled into the model to reflect the multiple processes in CBM extraction. The finite element method is employed to numerically solve the PDEs. The proposed model is verified by comparing its simulation results to a set of well production data from Southern Qinshui Basin in Shanxi Province, China. Great consistency is observed, showing the satisfactory accuracy of the model for CBM extraction. After that, the difference between various stimulation patterns is presented by simulating the CBM extraction process with different stimulation patterns including (1) unstimulated coalbed; (2) double-wing fracture + NSRD; (3) multiple RFs + NSRD; (4) SRD + NSRD and (5) multiple RFs + SRD + NSRD. The results suggest that Pattern (5) (often formed by CO2 fracking) boosts the efficiency of CBM extraction because it generates a complex fracture network at various scales by both increasing the number of radial fractures and activating the micro-fractures in coal blocks. Sensitivity analysis is also performed to understand the influences of key factors on gas extraction from a stimulated coalbed with multiple domains. It is found that the distinct properties of different domains originate various evolutions, which in turn influences the CBM production. Ignoring thermal effects in CBM extraction will either overestimate or underestimate the production, which is the net effect of thermal strain and non-isothermal sorption. The proposed model provides a useful approach to accurately evaluate CBM extraction by taking the complex evolutions of coalbed properties and the interactions between different components and domains into account. The importance of multidomain and thermal effects for CBM reservoir simulation is also highlighted.
Aspects of Multiscale Flow Simulation with Potential to Enhance Reservoir Engineering Practice
Authors: Kumar Khataniar Sanjoy , De Brito Dias Daniel , Xu Rong 
Published: October 2021

Abstract
A new implementation of a multiscale sequential fully implicit (MS SFI) reservoir simulation method is applied to a set of reservoir engineering problems to understand its utility. An assessment is made to highlight areas where the approach brings substantial advantage in performance as well as address problems not successfully resolved by existing methods.
This work makes use of the first ever implementation of the multiscale sequential fully implicit method in a commercial reservoir simulator. The key features of the method and implementation are briefly discussed. The learnings gained during field testing and commercialization on about forty real world models is illustrated through simpler, but representative data sets, available in the public domain. The workhorse robust fully implicit (FI) method is used as a reference for benchmarking. The MS SFI method can faithfully reproduce FI results for black oil problems.
We conclude that the MS SFI method has the capability to support reservoir engineering decision making especially in the areas of subsurface uncertainty quantification, representative model selection, model calibration and optimization. The MS SFI method shows immense potential for handling prominent levels of reservoir heterogeneity. The challenge of including fine-scale heterogeneity, which is often overlooked, when scaling up EOR processes from laboratory to field, appears to have found a practical solution with a combination of MS SFI and high-performance computing (HPC).