bluuebunny/crossref_metadata_2025_split huggingface dataset:
EXTENSIVE GENETIC INTERACTIONS (EPISTASIS) LINKED TO ALCOHOL USE DISORDER IN A HIGH-RISK POPULATION
Authors: Listopad Stanislav , Peng Qian 
Published: December 2024

AbstractAlcohol use disorder (AUD) is known to have a significant genetic component, yet there remains a substantial gap between its heritability and findings from genome-wide association studies. One potential factor contributing to this gap may be genetic interactions, or epistasis, a largely unexplored aspect in the context of AUD. The aim of this study was to investigate the role of epistasis in AUD susceptibility and severity among American Indians, a population that exhibits the highest rates of AUD among all ethnic groups in the U.S. We began by identifying genes previously linked to alcohol dependence and AUD, then expanded this gene set through biological networks, ultimately comprising 3,736 genes and regulatory elements. The final gene set was mapped to over 476K variants in an American Indian cohort of 742 individuals. We performed a pairwise genetic interaction association analysis on the variant set, followed by a bi-clustering procedure to group the interacting SNP pairs into interacting intervals. A total of 114 interacting pairs of genes and regulatory elements were identified to be significantly associated with AUD severity. These genes were enriched for immune system, cell adhesion, neuronal, and disease pathways. Their expressions were particularly enriched in midbrain GABAergic neurons. Our study represents the first large-scale genetic interaction study of AUD in any population. Our findings suggest that epistasis may significantly contribute to the development and progression of AUD.
Epigenetic Disordering Drives Stemness, Senescence Escape and Tumor Heterogeneity
Authors: Magnani Elena , Macchi Filippo , Randic Tijana , Chen Charlene , Madakashira Bhavani , Ranjan Shashi , Eski Sema Elif , Singh Sumeet P. , Sadler Kirsten C. 
Published: December 2024

SummaryTumor heterogeneity is the substrate for tumor evolution and the linchpin of treatment resistance. Cancer cell heterogeneity is largely attributed to distinct genetic changes within each cell population. However, the widespread epigenome repatterning that characterizes most cancers is also highly heterogenous within tumors and could generate cells with diverse identities and malignant features. We show that high levels of the epigenetic regulator and oncogene, UHRF1, in zebrafish hepatocytes rapidly induced methylome disordering, loss of heterochromatin, and DNA damage, resulting in cell cycle arrest, senescence, and acquisition of stemness. Reducing UHRF1 expression transitions these cells from senescent to proliferation-competent. The expansion of these damaged cells results in hepatocellular carcinomas (HCC) that have immature cancer cells intermingled with fibroblasts, immune and senescent cells expressing high UHRF1 levels, which serve as reservoirs for new cancer cells. This defines a distinct and heterogenous HCC subtype resulting from epigenetic changes, stemness and senescence escape.
Immune cell single-cell RNA sequencing analyses link an age-associated T cell subset to symptomatic benign prostatic hyperplasia
Authors: Broman Meaghan M. , Lanman Nadia A. , Vickman Renee E. , Cresswell Gregory M. , Kothandaraman Harish , Kolliegbo Andree , Paez Paez Juan Sebastian , Glaser Alexander P. , Helfand Brian T. , Henry Gervaise , Strand Douglas W. , Franco Omar E. , Crawford Susan E. , Hayward Simon W. , Ratliff Timothy L. 
Published: December 2024

AbstractBenign prostatic hyperplasia (BPH) is among the most common age-associated diseases in men; however, the contribution of age-related changes in immune cells to BPH is not clear. The current study determined that an age-associated CD8+T cell subset (Taa) with high Granzyme K (GZMKhi) and low Granzyme B (GZMBlow) gene expression infiltrate aged human prostates and positively correlate with International Prostate Symptom Score (IPSS). A velocity analysis indicated that CD8+T cell differentiation is altered in large BPH prostates compared to small age-matched prostates, favoring Taa accumulation. In vitro granzyme K treatment of human BPH patient-derived large prostate fibroblasts increased secretion of pro-inflammatory senescence-associated secretory phenotype (SASP)-associated cytokines. These data suggest that granzyme K-mediated stimulation of prostate stromal fibroblast SASP cytokine and chemokine production promotes prostate immune cell recruitment and activation. Overall, these results connect symptomatic BPH with immune aging.
A microphysiological system of sterile injury demonstrates neutrophil reverse migration via macrophage-derived extracellular vesicle crosstalk
Authors: Babatunde Kehinde Adebayo , Oluwadamilola Babatunde Fatimat , Ahmed Adeel , Salgado-Pabon Wilmara , Beebe David J , Kerr Sheena C 
Published: December 2024

ABSTRACTPersistent neutrophilic inflammation can lead to tissue damage and chronic inflammation, contributing to non-healing wounds. The resolution phase of neutrophilic inflammation is critical to preventing tissue damage, as observed in diseases characterized by influx of neutrophils such as atherosclerosis and non-healing wounds. Animal models have provided insight into resolution of neutrophilic inflammation via efferocytosis and reverse migration (rM); however, species-specific differences and complexity of innate immune responses make translation to humans challenging. Thus, there is a need forin vitrosystems that can elucidate mechanisms of resolution of human neutrophilic inflammation. Here, we developed a human microphysiological system (MPS) to mimic an inflammatory sterile injury (SI) microenvironment to study the role of macrophage derived extracellular vesicles (M-EVs) in determining the resolution of inflammation via neutrophil rM. The MPS integrates a human umbilical vein endothelial cell (HUVEC) lined lumen, injury site spheroid, human neutrophils, macrophages and macrophage derived EVs to investigate the role of M-EVs in neutrophil rMin vitro. The key features of the MPS enabled us to demonstrate that EVs derived from macrophage subsets modulate migratory behavior in primary neutrophils differently in specific inflammatory microenvironments. Importantly, we identified a new mechanism underlying neutrophil rM via M-EV, where neutrophils exposed to M2-EV-derived IL-8 migrate away from the SI site upon reaching the site, using the SI MPS. Overall, our SI MPS system demonstrates a reverse migratory pattern in human primary neutrophils, advancing the study of the resolution of inflammation via M-EVs.
GnT motifs: short nucleotide tracts of ≥8bp that can increase T:A→G:C mutation rates >1000-fold in bacteria
Authors: Horton James S. , Cherry Joshua L. , Waugh Gretel , Taylor Tiffany B. 
Published: December 2024

AbstractNucleotides across a genome do not mutate at equal frequencies. Instead, specific nucleotide positions can exhibit much higher mutation rates than the genomic average due to their immediate nucleotide neighbours. These ‘mutational hotspots’ can play a prominent role in adaptive evolution, yet we lack knowledge of which short nucleotide tracts drive hotspots. In this work, we employ a combinatorial approach of experimental evolution withPseudomonas fluorescensand bioinformatic analysis of variousSalmonellaspecies to characterise a short nucleotide motif (≥8bp) that drives T:A→G:C mutation rates >1000-fold higher than the average T→G rate in bacteria. First, we experimentally show that homopolymeric tracts (≥3) of G with a 3’ T frequently mutate so that the 3’ T is replaced with a G, resulting in an extension of the guanine tract, i.e., GGGGT → GGGGG. We then demonstrate that the potency of this T:A→G:C hotspot is dependent on the nucleotides immediately flanking the GnT motif. We find that the dinucleotide pair immediately 5’ to a G4tract and the nucleotide immediately 3’ to the T strongly affect the T:A→G:C mutation rate, which ranges from ∼5-fold higher than the typical rate to >1000-fold higher depending on the flanking elements. Therefore the T:A→G:C hotspot motif is a product of several modular nucleotide components (1-4bp in length) which each exert a significant effect on the mutation rate of the GnT motif. This work advances our ability to accurately identify the position and quantify the mutagenicity of hotspot motifs predicated on short tracts of nucleotides.
Unified interpretation of the muon
g−2
anomaly, the 95 GeV diphoton, and
bb¯
excesses in the general next-to-minimal supersymmetric standard model
Authors: Cao Junjie , Jia Xinglong , Lian Jingwei 
Published: December 2024

We investigate three intriguing anomalies within the framework of the general next-to-minimal supersymmetric standard model. These anomalies include a significant deviation of the experimental results for the muon anomalous magnetic moment from its standard model prediction, with a confidence level of 5.1σ; a joint observation by the CMS and ATLAS Collaborations of a diphoton excess with a local significance of 3.1σ in the invariant mass distribution around 95.4 GeV; and a reported excess in the bb¯ production at the Large Electron-Positron Collider with a local significance of 2.3σ. Through analytical and numerical analyses, we provide unified interpretations across an extensive parameter space that remain consistent with current experimental restrictions from data on the Higgs boson at 125 GeV, B-physics measurements, and dark matter observables, as well as existing searches for supersymmetry and extra Higgs bosons. We attribute the muon anomaly to loops involving muon-smuon-neutralino and muon-sneutrino-chargino interactions, while attributing the diphoton and bb¯ excesses to the resonant production of a singlet-dominated scalar. These proposed solutions are poised for experimental tests at the high-luminosity LHC and future linear colliders.

Published by the American Physical Society
2024
How celebrity attachment influences event participation intentions: from the customer-relationship theory and attachment theory approaches
Authors: Tran Phuong Kim Thi , Ho-Mai Nhi Thao , Nguyen Nhi Uyen Thi , Mai Uyen Phuong Thi , Nguyen Nhi Uyen Ngoc , Bui Duong Hai Thi , Le Huy Van , Tran Vinh Trung 
Published: December 2024

PurposeFrom the customer-relationship theory and attachment theory approaches, this study proposes a serial mediation model to examine how celebrity attachment influences event attendees' intentions in the celebrity endorsement process in the context of events.Design/methodology/approachPaper-based and online surveys were used to collect data from 759 Vietnamese respondents, aged 15 and above, who followed domestic or international celebrities and were interested in various events taking place in Vietnam. A serial multiple mediation model was evaluated through covariance-based structural equation modeling.FindingsThe results confirmed the cognitive, affective and hybrid cognitive-affective pathways among antecedents, celebrity attachment and event participation intentions.Research limitations/implicationsFuture studies need to validate these findings across diverse cultural settings and larger participant pools to enhance their applicability. Exploring celebrity endorsement for events from an international follower perspective could offer valuable insights. Future research should consider these factors when interpreting results. It may benefit from conducting longitudinal or mixed-method studies to improve generalizability. Additional moderating variables are necessary, as research on the celebrity endorsement process for events evolves.Originality/valueThis study contributes to the literature on celebrity endorsement within event marketing, emphasizing the customer-brand relationship and attachment theory. It extends existing research that primarily examines how celebrity attachment influences event attendees' intentions in the celebrity endorsement process by validating a serial mediation model.
Exploring the intersection of management control systems and gender equality: an integrative literature review
Authors: Hosoda Masahiro , Toyosaki Hitomi 
Published: December 2024

Purpose
This study aims to provide a deeper understanding of how management control systems (MCSs) can contribute to achieving gender equality.

Design/methodology/approach
The researchers undertook an integrative review of the literature, focusing on the characteristics of MCSs for the development of female managers as well as highlighting the deployment of MCSs by them. Nineteen (19) articles were selected from a database in terms of specified criteria, following which they were analysed in terms of the identified MCSs.

Findings
The study found interrelations between MCSs and the internal as well as external factors that promote the realisation of gender equality while highlighting the characteristics of the utilisation of MCSs by female managers. Future research agendas related to these two aspects are also suggested.

Originality/value
Theoretically, the study is significant because it offers new insights for analysing gender and gender equality issues in MCSs research, thus opening up fresh dimensions for future research. In terms of praxis, the study promotes gender symmetry in companies by addressing gender equality from the perspective of MCSs. The research also charts a novel course for promoting gender equality in the workplace regarding how female managers use MCSs.
Cross-level interaction mechanism for high growth among digital start–ups: an fsQCA analysis
Authors: Li Honglei , Wang Hongyu , Yang Ziyu , Guo Changwei 
Published: December 2024

Purpose
In the field of digital entrepreneurship, achieving high growth in digital start-ups is crucial. This paper aims to explore how causal conditions at the individual, organizational and environmental levels interact in the growth process of digital start-ups. Furthermore, it uses a configurational approach to conduct a holistic analysis of the cross-level interaction mechanisms that enable digital start-ups to achieve high growth.

Design/methodology/approach
Based on a holistic perspective, the data collection included 57 representative cases of digital start-ups in China in the past decade (2014–2023). A fuzzy set qualitative comparative analysis was conducted to explore the cross-level interaction mechanism of the three-dimensional causal conditions of the environment, organization and individual entrepreneurs on high growth among digital start-ups.

Findings
This study found three models for digital start-ups to achieve high growth. These models − the resource network orchestration model, the innovation resource development model and the entrepreneurial spirit coherence model, respectively, reflect the multiple paths of high growth achieved by various digital start-ups based on their entrepreneurial environment, organizational capability and the personality of the founder. This reflects that digital start-ups face different conditions and entrepreneurial situations, with differing cross-level interaction mechanisms for achieving high growth.

Practical implications
First, digital start-ups should focus on strengthening their digital resource integration capabilities to lay the foundation for their subsequent high-growth path selection. Second, entrepreneurs need to adopt a holistic perspective to change or strengthen the causal conditions required to achieve high growth. Third, emphasis should be placed on the coherence between the entrepreneur’s need for achievement and the internal entrepreneurial culture.

Originality/value
First, this study contributes to the literature on high growth among digital start-ups by introducing a cross-level interaction mechanism comprising environmental, organizational and entrepreneurial causal conditions. Second, it reveals that individual factors at the environmental, organizational and entrepreneurial levels are not necessary conditions for high entrepreneurial growth and that digital resource integration capabilities play a more universal role in achieving high digital start-up growth. Finally, the study provides a cross-level holistic explanation of how digital start-ups can achieve high growth and reveals various configuration models conducive to high growth, thus offering practical insights for businesses.
Are female entrepreneurs ambidextrous leaders? A qualitative study through the lens of social role theory
Authors: Oluwafemi Tolulope Busola 
Published: December 2024

Purpose
The purpose of this study is to examine how female leaders demonstrate ambidextrous leadership behaviors amid perceived role incongruity.

Design/methodology/approach
This study adopted an exploratory, qualitative approach. Data is collected via interviews with 14 female business owners and analyzed thematically.

Findings
As entrepreneurs, the female leaders draw on both communal and agentic traits; they also demonstrate both opening and closing leadership behaviors and can switch between leadership behaviors and traits as may be required by the situation. Experiences of gender-specific challenges were less visible for this group, and the female leaders consider all actions taken to ensure business venture success as superseding role expectations of any kind.

Research limitations/implications
The research design may limit the extent to which the findings can be generalized.

Practical implications
The study provides insights on issues such as gender equality (SDG 5), reduced inequalities (SDG 10) and uncovers ways to support female leaders or encourage young girls to embrace leadership opportunities and business ownership. Current and future female leaders may also find this study useful to boost self-efficacy and navigate gender-specific leadership challenges.

Originality/value
This study shows that female entrepreneurs are ambidextrous leaders and gender-related challenges are less visible for female entrepreneurs/business owners. It highlights that consideration for business success is more important to female entrepreneurs than concerns about role incongruity. Also, the study shows that female entrepreneurs need to balance communal traits and agentic traits as well as opening and closing leadership behaviors to successfully lead their business ventures.
The double-edged sword effect of artificial intelligence awareness among hotel employees
Authors: Liu Shengmin , Cheng Pengfan 
Published: December 2024

Purpose
With its continuous development and application in the hotel industry, artificial intelligence (AI) is gradually replacing many jobs traditionally performed by humans. This research aims to understand how this threat and opportunity of substitution affects hotel employees’ behavioral decision-making.

Design/methodology/approach
This study uses a structural equation model, ordinary least squares and bootstrapping method to analyze the data collected with a field study and a scenario experiment from star-hotels in Shanghai, Paris and Seoul.

Findings
The results discovered that employees’ AI awareness has a positive relationship with their work engagement and AI boycott through two paths. The promoting path involves recovery level, while the hindering path includes job insecurity. In addition, the estimates showed that AI awareness has a great indirect effect on work engagement or AI boycott when innovativeness as a job requirement is high.

Practical implications
The findings offer insights to help hotels optimize the relationship between AI and hotel human workers while providing valuable implications for addressing behavioral dilemmas faced by hotel employees in the era of AI.

Originality/value
By integrating the behavioral decision-making literature with the conservation of resources theory, the study focuses on the dual mechanisms – challenging and hindering – through which AI awareness influences hotel employees’ coping strategies.
Managing institutional multiplicity: a case of a bilateral development agency’s regional office
Authors: Fushimi Katsutoshi 
Published: December 2024

PurposeDrawing on institutionalism supplemented by a micro-political perspective, this study explores how a bilateral development agency’s (BDA’s) regional office manages institutional multiplicity, a situation where organisations are embedded into the institutional environments of their headquarters and multiple host countries.Design/methodology/approachI remotely conducted semi-structured in-depth interviews with 20 staff members of a BDA’s regional office in South-East Europe. Reflexive Thematic Analysis was used to analyse the interview data.FindingsTwo themes are developed. One is an institutional decomposition strategy. The subject office decomposes institutional multiplicity into more manageable multiple institutional dualities by deploying local representatives to host countries. The other is the division of duties to demonstrate legitimacy. The division designates who in the office demonstrate legitimacy in which institutional environment. These proactive actions by the office (i.e., the decomposition and the division) question the institutionalist assertion that external institutional conditions determine organisational behaviour.Research limitations/implicationsThe findings may not directly apply to other public sector organisations because BDAs’ overseas offices are “donors” for their host countries. In addition, themes developed in the context of South-East Europe may not be generalisable to other regions.Practical implicationsBDA staff members should understand that institutional decomposition through the deployment of local representatives is a rational strategy to deal with complex conditions of institutional multiplicity. They should also understand that experienced local representatives are required to achieve this strategy.Originality/valueThis is the first empirical study to examine how a public sector organisation’s regional office manages complex institutional multiplicity from a micro-macro combined perspective.
Transforming the public service via digital leadership in Uganda
Authors: Kyambade Mahadih , Namatovu Afulah 
Published: December 2024

PurposeThe purpose of this study was to explore the competencies required for digital leaders and understand how public tertiary and higher education institutions are conceiving the concept through their curriculum and instruction.Design/methodology/approachFive in-depth qualitative interviews that used grounded theory were employed, and a constant comparison approach to data analysis was used.FindingsThere was consensus among the majority of interviewees for the competencies required for digital leaders but differing opinions on the methods to provide instruction.Practical implicationsThis study suggests that technical skills are less relevant than an open mindset, willingness to try and ability to employ new ways of thinking into a workforce culture. Data also revealed that the particular leadership style used should be based on the context of the organization and the boundaries that exist within the culture.Originality/valueThis study adds valuable insights into the concept of digital leadership and how public tertiary and higher education institutions are providing instruction on the topic.