bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic adverse events after screening of retinopathy of prematurity with mydriatic
Authors: Obata Shumpei , Imamura Taku , Kakinoki Masashi , Yanagi Takahide , Maruo Yoshihiro , Ohji Masahito 
Published: September 2021

PurposeTo evaluate systemic adverse events after screening for retinopathy of prematurity (ROP) performed with mydriatic.MethodsThis was a retrospective case series study. Medical records of consecutive patients who underwent screening for ROP with 0.5% phenylephrine and 0.5% tropicamide eyedrops were retrospectively reviewed. The score of abdominal distention (0–5), volume of milk sucked and volume of stool, along with systemic details (pulse and respiration rates, blood pressure and number of periods of apnea) were collected at 1 week and 1 day before ROP examination, and at 1 day after examination. Results were compared between the days before and after examination. Correlation between body weight at the time of examination and the score of abdominal distention was examined. The numbers of infants with abdominal and/or systemic adverse events were compared between pre- and post-examination periods.ResultsEighty-six infants met the inclusion criteria. The score of abdominal distention increased from 2.0 at 1 day before examination to 2.3 at 1 day after examination (p = 0.005), and the number of infants who had worsened abdominal distension increased after examination (p = 0.01). Infants with lower body weight had a higher score of abdominal distention (p < 0.0001, r = −0.57). The number of infants with reduced milk consumption increased after examination (p = 0.0001), as did the number of infants with decreased pulse rate (p = 0.0008).ConclusionsScreening for ROP with mydriatic may have adverse effects on systemic conditions. Infants should be carefully monitored after ROP screening with mydriatic.
Maternal PCOS status and metformin in pregnancy: Steroid hormones in 5–10 years old children from the PregMet randomized controlled study
Authors: Hanem Liv Guro Engen , Salvesen Øyvind , Madsen André , Sagen Jørn V. , Mellgren Gunnar , Juliusson Petur Benedikt , Carlsen Sven Magnus , Vanky Eszter , Ødegård Rønnaug 
Published: September 2021

Objective
Polycystic ovary syndrome (PCOS) is a common endocrine disorder, with potential effects on offspring both genetically and through altered intrauterine environment. Metformin, which ameliorate hormonal disturbances in non-pregnant women with PCOS is increasingly used in pregnancy. It passes the placenta, and the evidence on potential consequences for offspring endocrine development is scarce. We explore the potential effects of maternal PCOS status and intrauterine metformin exposure on offspring steroid hormone levels.

Design
This is a follow-up study of 5–10 years old children from the PregMet-study–a randomized controlled trial comparing metformin (2000 mg/day) to placebo during PCOS pregnancies. Of the 255 children invited, 117 (46%) were included.

Methods
There was no intervention in this follow-up study. Outcomes were serum levels of androstenedione, testosterone, SHBG, cortisol, 17-hydroxyprogesterone, 11-deoxycortisol and calculated free testosterone converted to gender-and age adjusted z-scores from a Norwegian reference population. These were compared in i) placebo-exposed children versus children from the reference population (z-score zero) by the deviation in z-score by one-sample t-tests and ii) metformin versus placebo-exposed children by two-sample t-tests. Holm-Bonferroni adjustments were performed to account for multiple endpoints.

Results
Girls of mothers with PCOS (n = 30) had higher mean z-scores of androstenedione (0.73 (95% confidence interval (CI) 0.41 to 1.06), p<0.0001), testosterone (0.76 (0.51 to 1.00), p<0.0001), and free testosterone (0.99 (0.67 to 1.32), p<0.0001) than the reference population. Metformin-exposed boys (n = 31) tended to have higher 11-deoxycortisol z-score than placebo-exposed boys (n = 24) (mean difference 0.65 (95% CI 0.14–1.17), p = 0.014).

Conclusion
Maternal PCOS status was associated with elevated androgens in 5- to 10-year-old daughters, which might indicate earlier maturation and increased risk of developing PCOS. An impact of metformin in pregnancy on steroidogenesis in children born to mothers with PCOS cannot be excluded. Our findings need confirmation in studies that include participants that have entered puberty.
Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
Authors: Ou Xuelan , Xia Tianqin , Yang Chunyan , Yu Chunlei , Zhang Shipeng , Huang Rong , Chen Chuan , Zhou Chunyang 
Published: September 2021

Abstract
As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 μmol/L P-A for 24 h; the release of H2S was the largest. After 100 μmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.
Morphophysiological changes and reactive oxygen species metabolism inCorchorus olitoriusL. under different abiotic stresses
Authors: Rahman Khussboo , Rahman Mira , Ahmed Naznin , Alam Md. Mahabub , Rahman Anisur , Islam Md. Mahbubul , Hasanuzzaman Mirza 
Published: September 2021

AbstractAbiotic stress has become an alarming issue for plant survival due to the constant changes in the environment. Abiotic stresses such as drought, salt, waterlogging, and heavy metals largely influence plant growth and development that finally reduce crop productivity. The present study was carried out to investigate the responses of jute (Corchorus olitoriuscv. O-9897) plant under different abiotic stresses. At 15th days after sowing plants were exposed to different abiotic stresses for various duration. Two doses of NaCl (200 and 400 mM) were applied to impose salt stress, while two doses of CdCl2(2 and 4 mM) were applied for cadmium (Cd) stress. Waterlogging stress was applied for 5 and 15 days. Whereas drought stress was imposed on plants for 10 and 15 days. Leaf relative water content, SPAD value, plant height, above ground fresh and dry weight, leaf area, and stem diameter decreased upon exposure to salt, water deficit, Cd, and waterlogging stresses. These abiotic stresses resulted in oxidative damage which was evident by the increased levels of lipid peroxidation, H2O2, and electrolyte leakage (EL) together with altered antioxidant enzymes activities and glyoxalase system which are crucial for plants to fight against oxidative damage. Both duration of waterlogging and drought stress drastically affected plant morphophysiology, whereasC. olitoriuscould tolerate moderate level of salt (200 mM NaCl) and Cd (2 mM CdCl2). So the present study reveals that abiotic stresses cause substantial damages to the morphophysiology and oxidative stress tolerance ofC. olitoriuswhere the higher doses of NaCl and CdCl2as well as the increased duration of waterlogging and drought resulted in more deleterious effect.
Synthesis and extraction routes of allelochemicals from plants and microbes: A review
Authors: Singh Apurva Anand , Rajeswari Gunasekaran , Nirmal Louis Anto , Jacob Samuel 
Published: September 2021

Abstract
Allelopathy, a complex phenomenon has unveiled both stimulatory and inhibitory effects in plant processes that are mediated by the release of certain chemical compounds commonly known as allelochemicals. Allelochemicals, a form of bioactive secondary metabolites are produced by a diverse group of plants and microbes in response to biotic and abiotic stress. It ranges from a simple hydrocarbon to complex polycyclic aromatic compounds like phenol, flavonoids, tannins, steroids, amino acids, alkaloids and quinones. These plant bioactive compounds are released into the environment via decomposition, exudation, leaching and volatilization that play a significant role in regulating the intra-specific or inter-specific relations with counterparts. A wide variety of methods has been proposed for analyzing the basic mechanism and overall effect of allelochemicals. However, the lack of a reliable and effective method to identify their molecular mode of action and their modulation in the metabolic pathway still remains as a great challenge. From a commercial perspective, these allelochemicals are deemed to be better candidates for green natural herbicides and weedicides that are proven to be environment friendly, unlike synthetic chemicals. In order to pave a way for the economic viability of these chemicals, a basic understanding of their chemistry is inevitable. This review article is focused to give an in-depth understanding of metabolic pathways genes responsible for the elicitation/secretion and the adoption of a suitable downstream process and analytical techniques that can intensify the process.
Promoting and Optimizing the Use of 3D-Printed Objects in Spontaneous Recognition Memory Tasks in Rodents: A Method for Improving Rigor and Reproducibility
Authors: Inayat Mehreen , Cruz-Sanchez Arely , Thorpe Hayley H. A. , Frie Jude A. , Richards Blake A , Khokhar Jibran Y. , Arruda-Carvalho Maithe 
Published: September 2021

AbstractSpontaneous recognition memory tasks are widely used to assess cognitive function in rodents and have become commonplace in the characterization of rodent models of neurodegenerative, neuropsychiatric and neurodevelopmental disorders. Leveraging an animal’s innate preference for novelty, these tasks use object exploration to capture the what, where and when components of recognition memory. Choosing and optimizing objects is a key feature when designing recognition memory tasks. Although the range of objects used in these tasks varies extensively across studies, object features can bias exploration, influence task difficulty and alter brain circuit recruitment. Here, we discuss the advantages of using 3D-printed objects in rodent spontaneous recognition memory tasks. We provide strategies for optimizing their design and usage, and offer a repository of tested, open-source designs for use with commonly used rodent species. The easy accessibility, low-cost, renewability and flexibility of 3D-printed open-source designs make this approach an important step toward improving rigor and reproducibility in rodent spontaneous recognition memory tasks.
Salt frost attack on concrete: the combined effect of cryogenic suction and chloride binding on ice formation
Authors: Müller Matthias , Ludwig Horst-Michael , Hasholt Marianne Tange 
Published: September 2021

AbstractScaling of concrete due to salt frost attack is an important durability issue in moderate and cold climates. The actual damage mechanism is still not completely understood. Two recent damage theories—the glue spall theory and the cryogenic suction theory—offer plausible, but conflicting explanations for the salt frost scaling mechanism. The present study deals with the cryogenic suction theory, which assumes that freezing concrete can take up unfrozen brine from a partly frozen deicing solution during salt frost attack. According to the model hypothesis, the resulting saturation of the concrete surface layer intensifies the ice formation in this layer and causes salt frost scaling. In this study an experimental technique was developed that makes it possible to quantify to which extent brine uptake can increase ice formation in hardened cement paste (used as a model material for concrete). The experiments were carried out with low temperature differential scanning calorimetry, where specimens were subjected to freeze–thaw cycles while being in contact with NaCl brine. Results showed that the ice content in the specimens increased with subsequent freeze–thaw cycles due to the brine uptake at temperatures below 0 °C. The ability of the hardened cement paste to bind chlorides from the absorbed brine at the same time affected the freezing/melting behavior of the pore solution and the magnitude of the ice content.
The Role of External Knowledge in the Innovative Activities of Agricultural Biotechnology Multinationals
Authors: Ribeiro Fernanda Teixeira Franco , Figueira Mariane , Calegario Cristina Lelis Leal 
Published: September 2021

Objective: To evaluate the influence of external knowledge absorbed on the potential to innovate of multinational companies (MNCs), in the genetically modified (GM) seed sector from 2000 to 2018.
Method: A quantitative approach was applied. Data was composed of the main patents filed by MNCs in the GM seed sector, collected from the Espacenet database, the MNCs’ annual reports, and the INPI database. Variables were analyzed using panel regression model.
Main results: Results showed that the MNCs’ own resources, as well as the external knowledge absorbed were individually significant. The Interactive variable was not significant in the model.
Relevance/ Originality: In addition to addressing literature gaps related for instance to the need to investigate the factors that influence the generation of innovation in multinationals, the present study focused on the agricultural biotechnology sector, which is of paramount importance for the innovations generated in agriculture.
Theoretical contributions: This study contributes by filling literature gaps related to the need to understand how important is the influence of the external knowledge and resources to the innovative capabilities of MNCs.
Social contributions: This paper helps managers be conscious of the fact that innovation is not only developed internally, involving the MNC’s own network, but in fact, innovation requires relationships with other companies, allowing the access of external knowledge and resources.
Okul çağındaki çocuklarda vitamin B12, folat ve demir eksikliği prevalansının değerlendirilmesi
Authors: KEFELİ Melike , ÖRNEK Zühal , KARDEŞ Hakan , EKEMEN Coşkun 
Published: September 2021

Amaç: Demir, vitamin B12 ve folat eksikliği tüm dünyada özellikle çocuklarda sık görülen mikrobesin eksikliklerindendir. Demir, vitamin B12 ve folat birçok enzimin yapısına girerek vücut metabolizması, hücre bölünme ve farklılaşması, immun sistemin düzenlenmesi, kemik gelişimi, algılama ve zekâ fonksiyonunda rol oynamaktadır. Çalışmada, çocuklarda serum demir, vitamin B12 ve folat eksikliği prevalansını araştırdık.
Gereç ve Yöntem: Zonguldak Bülent Ecevit Üniversitesi Tıp Fakültesi Hastanesi Genel Çocuk Polikliniği’ne başvuran 6-14 yaş arası toplam 392 çocukta serum demir düzeyi, demir bağlama kapasitesi, ferritin, vitamin B12, folat düzeyi ve tam kan sayımı çalışıldı. Sosyoekonomik düzey, beslenme şekli, fiziksel gelişimi, eğitim düzeyi gibi çeşitli parametreleri içeren anket düzenlendi.
Bulgular: Çalışmamız 223 kız, 169 erkek toplam 392 çocukla yapıldı. Olguların %60,5’inde demir eksikliği (DE), %33,4’ünde vitamin B12 eksikliği, %7,6’sında folat eksikliği ve yetersizliği saptandı. Olgular 6-10 yaş (n=211) ve 11-14 yaş (n=181) olarak iki grupta incelendiğinde vitamin B12 eksikliğinin görülme sıklığında adölesanlarda istatistiksel olarak anlamlı fark saptandı (p
Hubungan Screen Based Activity dan Perilaku Makan dengan Status Gizi Anak Usia 10-12 Tahun (Studi di SDI Darush Sholihin Kabupaten Nganjuk)
Authors: Alfinnia Silvia , Muniroh Lailatul , Atmaka Dominikus Raditya 
Published: September 2021

ABSTRAK Latar Belakang: Anak usia sekolah mengalami peningkatan kebutuhan gizi untuk tumbuh kembang. Di usia ini, anak-anak bisa memilih makanan maupun media bermain sesuai keinginan mereka. Aktivitas menggunakan layar yang berlebih serta perilaku makan yang buruk dapat memicu terjadinya obesitas.Tujuan: Penelitian ini bertujuan untuk menganalisis hubungan Screen Based Activity (SBA) dan perilaku makan dengan status gizi anak usia sekolah.Metode: Penelitian ini merupakan penelitian observasional analitik dengan desain penelitian cross sectional. Penelitian dilakukan di SDI Darush Sholihin Kabupaten Nganjuk. Besar sampel sebanyak 48 siswa yang dipilih secara proportional random sampling. Pengumpulan data meliputi berat badan, tinggi badan, kuesioner SBA, Food Frequency Questionnaire (FFQ), serta food recall 2x24 jam. Data dianalisis menggunakan uji korelasi Spearman dan Kendall’s tau dengan nilai signifikansi 0,05.Hasil: Hasil penelitian menunjukkan bahwa tidak ada hubungan SBA (p=0,151), perilaku makan makanan pokok (p=0,101), perilaku makan lauk hewani (p=0,212), perilaku makan lauk nabati (p=0,829), perilaku makan sayuran (p=0,751) dan perilaku makan jajanan (p=0,109) dengan status gizi. Namun, terdapat hubungan perilaku makan buah (p=0,040) dengan status gizi.Kesimpulan: Konsumsi buah-buahan yang sering tanpa memperhatikan kandungan gula dan cara penyajian dapat memberikan risiko obesitas pada anak. Diperlukan pendidikan gizi kepada pihak sekolah maupun orang tua mengenai pembatasan SBA dan perilaku makan sehat terutama buah untuk mencapai tumbuh kembang yang optimal dan terhindar dari obesitas.
Determinan Pemberian ASI Eksklusif: Analisis Data Sekunder Survei Demografi dan Kesehatan 2017
Authors: Putri Adilah Anindito Difa , Naim Salsabila 
Published: September 2021

Latar Belakang: Angka Kematian Bayi merupakan salah satu masalah kesehatan utama di Indonesia. Pemberian ASI eksklusif kepada bayi merupakan salah satu cara efektif agar dapat menurunkan Angka Kematian Bayi di Indonesia. Cakupan ASI eksklusif pada tahun 2017 sebesar 52%, hal tersebut telah memenuhi minimal 50% dari target nasional namun cakupan ASI eksklusif menurun seiring dengan pertambahan usia anak.Tujuan: Penelitian ini bertujuan untuk menentukan faktor yang mempengaruhi pemberian ASI eksklusif di Indonesia.Metode: Penelitian ini menggunakan data sekunder Survei Demografi dan Kesehatan Indonesia 2017 dengan desain cross-sectional. Sampel penelitian berjumlah 1.435 yang terdiri dari ibu yang memiliki anak berusia 0-5 bulan terakhir yang masih hidup, tidak memiliki anak kembar, tinggal bersama anaknya dan masih menyusui. Analisis data menggunakan analisis bivariabel metode chi-square dan analisis multivariabel metode regresi logistik berganda.Hasil: Ibu yang memiliki anak yang berusia 0 – 1 bulan berpeluang 22,835 kali lebih tinggi untuk ASI eksklusif dibanding anak usia 4 – 5 bulan (OR 22,835; CI 95% 11, 033 – 47,261), begitu pula pada ibu yang memiliki anak yang berusia 2 – 3 bulan berpeluang tinggi untuk ASI eksklusif juga. Ibu dengan pendidikan tinggi 3,383 kali berpeluang lebih tinggi untuk ASI eksklusif dibanding ibu yang tidak sekolah (OR 3,383; CI 95% 0,999 – 11,461), hal yang sama berlaku pada ibu dengan pendidikan menengah dan pendidikan dasar yang memiliki peluang tinggi untuk ASI eksklusif juga. Ibu dengan tingkat status ekonomi atas berpeluang 1,670 kali lebih tinggi untuk ASI eksklusif dibanding ibu dengan tingkat status ekonomi bawah (OR 1,670; CI 95% 1,102 – 2,529), hal yang sama berlaku pada ibu dengan tingkat status ekonomi menengah yang memiliki peluang tinggi untuk ASI eksklusif juga.Kesimpulan: Hasil penelitian menunjukkan bahwa faktor-faktor yang mempengaruhi pemberian ASI eksklusif antara lain: usia anak, tingkat pendidikan ibu dan status ekonomi.
PERAN SENTRA INDUSTRI GERABAH MELIKAN DALAM PENGEMBANGAN KAWASAN PARIWISATA
Authors: Sundoro Gogor Aldi , Miladan Nur , Pamardhi-Utomo Rizon 
Published: September 2021

<p><em>Pariwisata menjadi strategi yang telah banyak diterapkan untuk meningkatkan nilai tambah sentra industri rumah tangga.</em><em> </em><em>Pemerintah Kabupaten Klaten juga menggunakan strategi tersebut dalam mengembangkan sektor industri rumah tangganya. Sentra Industri Gerabah Melikan merupakan salah satu sektor industri di Kabupaten Klaten yang diterapkan menjadi kawasan pariwisata. Kegiatan wisata di Sentra Industri Gerabah Melikan tidak lepas dari permasalahan, seperti permasalahan terkait fasilitas sentra yang kurang mendukung kegiatan wisata. Berdasarkan masalah tersebut</em><em>,</em><em> penelitian ini bertujuan </em><em>untuk </em><em>mengetahui komponen kesesuaian fasilitas kawasan Sentra Industri Melikan berdasarkan indikator kawasan pariwisata. Metode pendekatan yang digunakan dalam penelitian ini</em><em>,</em><em> yaitu pendekatan kuantitatif, sedangkan analisis yang digunakan merupakan analisis skoring. Pengumpulan data dilakukan dengan cara observasi, kuesioner, dan wawancara. Pembahasan komponen fasilitas sentra industri pada penelitian ini meliputi</em><em>,</em><em> area pembuatan gerabah wisata, area terdampak tungku pembakaran, ketersediaan jalan kawasan, jangkauan parkir wisata, ketersediaan tempat sampah kawasan, ketersediaan listrik kawasan, dan kualitas air bersih kawasan. Hasil dari penelitian ini menunjuk</em><em>k</em><em>an bahwa fasilitas Sentra Industri Gerabah Melikan m</em><em>emiliki</em><em> kesesuaian sedang dengan nilai 2,2 poin dari rentang 1-3 poin. Berdasarkan hasil tersebut dapat disimpulkan bahwa, fasilitas secara keseluruhan yang menjadi objek penelitian ini masih kurang mampu dalam mendukung Sentra Industri Gerabah Melikan sebagai kawasan pariwisata.</em></p>
Work Redesign for the 21st Century: Promising Strategies for Enhancing Worker Well-Being
Authors: Lovejoy Meg , Kelly Erin L. , Kubzansky Laura D. , Berkman Lisa F. 
Published: September 2021

Work is a key social determinant of population health and well-being. Yet, efforts to improve worker well-being in the United States are often focused on changing individual health behaviors via employer wellness programs. The COVID-19 health crisis has brought into sharp relief some of the limitations of current approaches, revealing structural conditions that heighten the vulnerability of workers and their families to physical and psychosocial stressors. To address these gaps, we build on existing frameworks and work redesign research to propose a model of work redesign updated for the 21st century that identifies strategies to reshape work conditions that are a root cause of stress-related health problems. These strategies include increasing worker schedule control and voice, moderating job demands, and providing training and employer support aimed at enhancing social relations at work. We conclude that work redesign offers new and viable directions for improving worker well-being and that guidance from federal and state governments could encourage the adoption and effective implementation of such initiatives. (Am J Public Health. 2021;111(10):1787–1795. https://doi.org/10.2105/AJPH.2021.306283 )