bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of long noncoding RNAs in SDF-1α-induced odontogenic differentiation of human dental pulp stem cells
Authors: Yao Bo , Cheng Xiaogang , Mei Xiaohan , Chou Jun , Zhang Beidi , Wang Jueyu , Yu Qing , xiao min 
Published: October 2021

Abstract
SDF-1α cotreatment was shown to have synergistic effects on BMP-2-induced odontogenic differentiation of human apical dental papillary stem cells (SCAP) both in vitro and in vivo. Long noncoding RNAs (lncRNAs) have an important role in the odontogenic differentiation of dental pulp stem cells (DPSCs). We examined the altered expression of lncRNAs in SDF-1α-induced odontogenic differentiation of DPSCs by lncRNA microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses. Alterations in lncRNA expression during odontogenic differentiation of DPSCs were identified. Moreover, bioinformatic analysis [Gene Ontology (GO) analysis and coding-noncoding gene coexpression (CNC) analysis] was conducted to predict the interactions of lncRNAs and identify core regulatory factors in SDF-1α-induced odontogenic differentiation of DPSCs. The microarray analysis identified 206 differentially expressed lncRNAs (134 lncRNAs with upregulated expression and 72 with downregulated expression) at 7 days post‑treatment. The data demonstrated that one lncRNA, AC080037.1, regulates SDF-1α-induced odontogenic differentiation of DPSCs. Our data showed that lncRNA AC080037.1 siRNA suppresses DPSCs migration and the expression of Rho GTPase induced by SDF-1α. Moreover, AC080037.1 knockdown significantly affected SDF-1α- and BMP-2-induced mineralized nodule formation and strongly suppressed Runt-related factor-2 (RUNX-2), DMP-1 and DSPP expression in DPSCs. Our results highlighted the significant involvement of one lncRNA, AC080037.1, in the positive regulation of the osteo/odontogenic differentiation of DPSCs and indicated that lncRNA AC080037.1 could be a potential target in regenerative endodontics.These findings reveal how lncRNAs are involved in regulating the SDF-1α-induced odontogenic differentiation of DPSCs, which may further advance translational studies of pulp tissue engineering.
Modified Fuzzy Logic Method for Materials Selection in Mechanical Engineering Design
Authors: Sharifan Mostafa , Abedian Ali , Razaghian Pardis 
Published: October 2021

Abstract
Materials selection is one of the primary steps in designing products, including mechanical ones. As a result, researchers are continuously developing simple, accurate, and feasible techniques to enhance the performance of the designed component by selecting the optimum materials from a wide variety of candidate materials. In this research work, a modified fuzzy approach referred to as Modified Fuzzy Logic Method (MFLM) is proposed to provide some highly appreciated advantages besides resolving some of the substantial shortcomings of the existing solutions. Customizing the method based on the designer’s knowledge and level of expertise, simultaneous consideration of qualitative and quantitative properties, and high accuracy in the ranking of candidate materials are some of the significant benefits of MFLM in comparison to the available alternative methods. While, complexity, high volume computational efforts, and more are amongst the resolved drawbacks. Moreover, the produced results show the power and simplicity of the technique in solving complex problems like materials selection for a gas turbine blade which involves a high range working temperature.
To Optimize Fiber Lay Length in OPGW Cables Used in Power Transmission Networks
Authors: Seraji Faramarz E. , Chaghazardi Ehsan , Emami Ali 
Published: October 2021

Abstract
In this paper, the optimal fiber length in optical ground wire (OPGW) cable during production process is determined. The results show that in OPGW cable, if the fiber stranding length is less than the maximum lay length, the ultimate tensile stress (UTS) percentage decreases, but if it is higher, it would increase the bending losses and uses more fiber length, which is economically not viable. The calculation results show that by determining the maximum fiber lay length, the UTS percentage of OPGW cable can be controlled for a desired value.
Degradation of Bisphenol-A: A Contaminant of Emerging Concern Using Catalytic Ozonation By Activated Carbon Impregnated Nanocomposite-Bimetallic Catalyst
Authors: Pokkiladathu Hariprasad , Farissi Salman , Sakkarai Anbazhagi , Muthuchamy Muthukumar 
Published: October 2021

Abstract
Rampant water pollution events and rising water demand caused by exponential population growth and depleting freshwater resources speak of an impending water crisis. The inability of conventional wastewater treatment systems to remove Contaminants of Emerging Concern (CEC) such as Bisphenol-A (BPA) beckons for new and efficient technologies to remove them from wastewater and water sources. Advanced oxidation processes such as ozonation are primarily known for their capability to oxidize and degrade organic entities in water but optimum mineralization levels were hard to achieve. In this study, we synthesized an activated carbon impregnated nanocomposite-bimetallic catalyst (AC/CeO2/ZnO) and used it along with ozonation to remove BPA from water. The catalyst was characterized using BET, XRD, FESEM, Raman spectra, and DLS studies. Catalytic ozonation achieved TOC removal 25% higher than non-catalytic ozonation process. The degradation pathway of BPA was proposed using LC-MS/LC-Q-TOF studies that found six main aromatic degradation byproducts. Catalytic ozonation and non-catalytic ozonation followed similar degradation pathways. The formation of persistent aliphatic acidic byproducts in the treated sample made TOC removal above 61% difficult.
Phenotype - Chemotype Correlation of the Herb Bletilla Rchb. f. Based on a Comprehensive Evaluation of Thirty- Three Geographic Populations
Authors: Huang Junfeng , Yuan Fang , Zhou Ming , Huang Tianyue , Zhang Yanjun , Liang Qiong 
Published: October 2021

Abstract
Background

The Bletilla genus of Orchidaceae includes plants with great economic value, among which B. striata is the main traditional medicinal plant, and its pseudobulb, known as BaiJi, was first recorded in Shennong's Classic of Materia Medica. However, there has been little systemic evaluation of the germplasm quality of Bletilla plants in China. In order to comprehensive evaluate the Bletilla resources in China and screen out the candidate phenotypic traits determining yield and/or quality of Bletilla, the variation of phenotypic indicators (pseudobulb, leaf, stem, inflorescence, flower) and active ingredients contents (polysaccharide, total phenolics and militarine) in different populations of B. striata and B. ochracea were investigated through 4 years' common-garden experiment.
Results

There were abundant phenotypic variations and significant differences among different populations in the morphological phenotypes, pseudobulb weight and main active ingredient contents. Five populations, including HNSZ, AHBZ, HBLT, HBSN and JSNJ, showed good prospects for industrial development, presenting higher quality in terms of yield and main active ingredient content. Pseudobulb yield, polysaccharide and total phenol content are positively correlated with phenotypic traits. Militarine content is negatively correlated with almost all indexes. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms.
Conclusions

Bletilla is not strictly geoauthentic medicinal plants. B. ochracea could be accepted as an alternative resource to B. striata. The best harvest period of Bletilla is the third year after cultivation. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms. These results provide important information required for the efficient screening and utilization of Bletilla germplasm resources.
Movement is governed by rotational population dynamics in spinal motor networks
Authors: Berg Rune , Lindén Henrik , Petersen Peter , Vestergaard Mikkel 
Published: October 2021

Abstract
Although the nervous system is elegantly orchestrating movements, the underlying neural principles remain unclear. Since flexor- and extensor-muscles alternate during movements like walking, it is often assumed that the responsible neural circuitry is similarly alternating in opposition. Here, we present ensemble recordings of neurons in the lumbar spinal cord that indicate that, rather than alternation, the population is performing a "rotation" in neural space, i.e. the neural activity is cycling through all phases continuously during the rhythmic behavior. The radius of rotation correlates with the intended muscle force. Since existing models of spinal motor control offer an inadequate explanation of rotation, we propose a new theory of neural generation of movement from which this and other unresolved issues, such as speed regulation, force control, and multi-functionalism, are conveniently explained.
Cut-out Risk Factor Analysis after Intramedullary Nailing for the Treatment of Extracapsular Fractures of the Proximal Femur: A Retrospective Study
Authors: Yoon Jae Youn , Park Saehan , Kim Taehyun , Im Gun-Il 
Published: October 2021

Abstract
BackgroundsThe basic method of surgical treatment for extracapsular hip fracture (ECF), including intertrochanteric fracture and basicervical fracture (BCF), is osteosynthesis. Intramedullary nails are among the most commonly used fixation devices for such fractures. Our study aimed to report the clinical outcomes of ECF treatment with two different nail devices and to analyze the risk factors associated with screw cut-out.MethodsWe retrospectively reviewed the medical records of 273 patients (300 cases) from a single institution who underwent surgical treatment for ECF between January 2013 and October 2018. Overall, 138 patients were eligible for the study and were divided into two groups according to the osteosynthesis device used. We evaluated the clinical outcomes of fracture surgery and performed univariate and multivariate regression analyses to identify risk factors associated with screw cut-out in each group.ResultsWe used proximal femoral nails (group 1) to treat 83 patients and cephalomedullary nails (group 2) to treat 55 patients. Nine cut-outs (group 1, 6 cases; group 2, 3 cases) occurred during follow-up. The patients’ high body mass index (BMI) (p=0.019), BCFs (p=0.007), non-extramedullary reduction in the anteroposterior and lateral planes (p=0.032 and p=0.043, respectively), and anti-rotation screw pull-outs (p=0.041) showed a positive correlation to screw cut-out in the univariate analysis of group 1. In group 2, only BCFs was positively correlated (p=0.020). In the multivariate analysis of group 1, the patients’ BMIs (p=0.024) and BCFs (p=0.024) showed a positive correlation with cut-out. Meanwhile, the multivariate analysis of group 2 did not identify any factors associated with cut-out.ConclusionsThe cut-out risk was significantly higher in the BCF cases, regardless of the nail design used. Considerable attention should be paid to treating such unstable fractures. We expect that new-generation nails using a helical blade, or interlocking derotation and interlocking screws may improve surgical outcomes.
Tailoring NIR-II photoluminescence of single thiolated Au25 nanoclusters by selective binding to proteins
Authors: Bertorelle Franck , Wegner David , Bakulić Martina Perić , Fakhouri Hussein , Comby-Zerbino Clothilde , Sagar Amin , Bernadó Pau , Resch-Genger Ute , Koutecky Vlasta Bonačić , Guével Xavier Le , Antoine Rodolphe 
Published: October 2021

Abstract
Atomically precise gold nanoclusters (Au NCs) are a fascinating class of nanomaterials that exhibit molecule-like properties and have outstanding photoluminescence (PL), which is highly dependent on their structure and chemical environment. Their ultrasmall size, molecular chemistry, and biocompatibility make them extremely appealing for selective biomolecule labeling in investigations of biological mechanisms at the cellular and anatomical levels. In this work, we report a simple route to incorporating a preformed Au25 nanocluster into a model bovine serum albumin (BSA) protein. A new approach combining small-angle X-ray scattering and molecular modeling provides a clear localization of a single Au25 within the protein to a cysteine residue on the gold nanocluster surface. Attaching Au25 to BSA strikingly modifies the PL properties with enhancement and a redshift in the second near-infrared window (NIR-II). An extensive study based on a bottom-up approach that uses mixed-ligand nanoclusters Au25pMBA(18−x)Cysx with x=2, 5, 18 supported by experimental data (steady state, time-resolved spectroscopy) and theoretical calculations (DFT) provides new hints at the origin of NIR-II emission in such nanoclusters and their subsequent enhancement when selectively binding to a cysteine-rich protein. This study paves the way to controlling the design of selectively sensitive probes in biomolecules through a ligand-based strategy to enable the optical detection of biomolecules in a cellular environment by live imaging.
Axillary Masses as Clinical Manifestations of Male Sweat Gland Carcinoma Associated with Extramammary Paget’s Disease and Accessory Breast Carcinoma: Two Cases Report and Literature Review
Authors: Wu Jing , Chen Hongdan , Dong Juanhui , Cao Yong , Li Wei , Zhang Fan , Zeng Xiaohua 
Published: October 2021

Abstract
BackgroundAccessory breast carcinoma and sweat gland carcinoma associated with extramammary Paget’s disease of the axilla in male are rare cases. In clinical diagnosis and treatment, it is necessary to determine the disease carefully and make a reasonable treatment strategy according to the patient’s own situation.Case presentationWe reported two male cases of special tumor with axillary mass as initial clinical symptom, one was diagnosed as accessory breast cancer and the other was diagnosed as sweat gland cancer associated with extramammary Paget’s disease. We did personalized treatment for the two diseases, hoping to provide a reference for the diagnosis and management of diseases with axillary nodules as the initial symptoms.ConclusionsThe reports of these two cases have the potential to provide reference and corresponding thinking for clinical differentiation of axillary lymphadenopathy caused by different causes and subsequent treatment. These two cases may further enrich the database of rare cases and provide some ideas for the treatment of axillary lymphadenopathy caused by different causes.
Cost and Outcomes of Routine HIV Care and Treatment: Public and Private Service Delivery Models Covering Low-Income Earners in South Africa
Authors: Long LC , Girdwood S , Govender K , Meyer-Rath G , Miot J 
Published: October 2021

Abstract
Background: While South Africa’s national HIV program is the largest in the world, it has yet to reach the UNAIDS 95-95-95 targets. To reach these targets the expansion of the HIV treatment program may be accelerated through the use private sector delivery models. This study identified three innovative non-governmental primary health care models providing HIV treatment, as well as two government primary health clinics that served similar populations. We estimated the resources used, and costs and outcomes of HIV treatment across these models.Methods: We conducted a cost-outcomes analysis by collecting patient-level resource usage and treatment outcomes through retrospective medical record reviews and a bottom-up micro-costing from the provider perspective (public or private payer). Outcomes were based on whether the patient was still in care at the end of the follow up period and viral load status, if available.Results: 376 patients were included across the five HIV treatment models. Across the three private sector models there are differences in the costs and outcomes of HIV treatment, but for two of the models they are within the range of the public sector primary health clinic results. The nurse-led model appears to be distinct from the others.Conclusion: The results suggest that it may be possible to expand the HIV treatment program by increasing access through private sector models. Depending on the model used it may be possible to achieve this at costs similar to those in the public sector while maintaining similar treatment outcomes.
Genome Sequencing of Inonotus Obliquus Reveals Insights Into Candidate Genes Involved In Secondary Metabolite Biosynthesis
Authors: Duan Yingce , Han Haiyan , Qi Jianzhao , Gao Jin-ming , Xu Zhichao , Wang Pengchao , Zhang Jie , Liu Chengwei 
Published: October 2021

Abstract
Background: Inonotus obliquus is an important edible and medicinal mushroom that was shown to have many pharmacological activities in preclinical trials, including anti-inflammatory, antitumor, immunomodulatory, and antioxidant effects. However, the biosynthesis of these pharmacological components has rarely been reported. The reason for this is that there have been no relevant reports on its genome information.Results: we report the genome of I. obliquus using a combined high-throughput Illumina NovaSeq with Oxford Nanopore PromethION sequencing platform. The de novo assembled 38.18 Mb I. obliquus genome was determined to harbor 12525 putative genes, with 81.83% of them having detectable sequence similarities to others available in public databases. Phylogenetic analysis revealed a close evolutionary relationship between I. obliquus and Fomitiporia mediterranea and Sanghuangporus baumii in the core Hymenochaetales clade. The I. obliquus genome was found to encode a repertoire of enzymes involved in carbohydrate metabolism, along with 135 cytochrome P450 proteins. Other annotated genes included those encoding key enzymes for secondary metabolite biosynthesis, such as those from polysaccharide, melanin, and triterpenoid pathways. Among them, the I. obliquus genome was particularly enriched in sesquiterpenoid biosynthesis genes and gene clusters.Conclusions: This study presents the first genome analysis of an important medical mushroom, I. obliquus, which can provide insights into the usefulness of this organism and its secondary metabolites in medicine.
Small Molecules Facilitate Single Factor-Mediated Sweat Gland Cell Reprogramming
Authors: Ji Shuaifei , Li Yan , Zhou Laixian , Xiang Jiangbing , Chen Huating , Liu Yiqiong , Gao Huanhuan , Fu Xiao-bing , Sun Xiaoyan 
Published: October 2021

Abstract
Background: Large skin defect caused severe disruption to the overall skin structure and irreversible damage of sweat gland (SG), resulting in destroy of physiological function of the skin. Reprogramming fibroblasts into sweat gland lineages may provide a promising strategy to obtain the desirable cell types for functional repair and regeneration of damaged skin. Methods: A direct reprogramming strategy of single factor ectodermal dysplasia antigen (EDA) in combination with small molecule cocktails promoting cell-fate conversion to regenerate SG cells from human dermal fibroblasts (HDFs) was developed. Quantitative PCR (qPCR), flow cytometry, calcium activity analysis, immunocytochemical analyses and starch-iodine sweat tests were used to characterize the phenotype, gene expression and function features of the induced sweat gland cells (iSGCs). Results: EDA overexpression drove HDFs toward SG lineages, and HDFs transfected with EDA acquired sweat gland cell phenotype in sweat gland conditional medium (SGM). Small-molecule cocktails favoring SG lineages greatly accelerated the SG fate program in SGM-treated HDF-EDA cells and further induced the regeneration of iSGCs. The HDFs-derived iSGCs exhibited similar phenotypical and functional features of native sweat gland cells. Eventually, in vivo transplantation experiment confirmed that iSGCs had the ability to regenerate SG structurally and functionally.Conclusion: We developed a SG reprogramming strategy to generate functional iSGCs from HDFs by using single factor EDA in combination with small molecules. The generation of iSGCs has important implications for in situ skin regeneration with restoration of sweat glands in the future.
Effects of Mild Traumatic Brain Injury On Resting State Brain Network Connectivity In Older Adults
Authors: Bittencourt Mayra , Horn Harm-Jan van der , Balart-Sánchez Sebastián A. , Marsman Jan-Bernard C. , Naalt Joukje van der , Maurits Natasha M. 
Published: October 2021

Abstract
Older age is associated with worsened outcome after mild traumatic brain injury (mTBI) and a higher risk of developing persistent post-traumatic complaints. However, the effects of mTBI sequelae on brain connectivity at older age and their association with post-traumatic complaints remain understudied. We analyzed multi-echo resting-state functional magnetic resonance imaging data from 25 older adults with mTBI (mean age: 68 years, SD: 5 years) in the subacute phase and 20 age-matched controls. Severity of complaints (e.g. fatigue, dizziness) was assessed using self-reported questionnaires. Group independent component analysis was used to identify intrinsic connectivity networks (ICNs). The effects of group and severity of complaints on ICNs were assessed using spatial maps intensity (SMI) as a measure of within-network connectivity, and (static) functional network connectivity (FNC) as a measure of between-network connectivity. Patients indicated a higher total severity of complaints than controls. Regarding SMI measures, we observed hyperconnectivity in left-mid temporal gyrus (cognitive-language network) and hypoconnectivity in the right-fusiform gyrus (visual-cerebellar network) that were associated with group. Additionally, we found interaction effects for SMI between severity of complaints and group in the visual(-cerebellar) domain. Regarding FNC measures, no significant effects were found. In older adults, changes in cognitive-language and visual(-cerebellar) networks are related to mTBI. Additionally, group-dependent associations between connectivity within visual(-cerebellar) networks and severity of complaints might indicate post-injury (mal)adaptive mechanisms, which could partly explain post-traumatic complaints (such as dizziness and balance disorders) that are common in older adults during the subacute phase.