bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tools to evaluate the impact of nutrition delivery on muscle and physical-related outcomes in critical care: a scoping review
Authors: Rozali Nur Shazleen Nisha , Azahari Nuraniza , Mohamed Nor Noraishah 
Published: February 2025

Understanding the impact of nutrition delivery on critically ill patient-centered outcomes is crucial. Patient-centered outcomes should be physical-related due to the natural course of catabolism experienced during critical illness. This review aims to map the existing tools used in research to evaluate the impact of nutrition delivery on muscle and physical-related outcomes among intensive care unit (ICU) patients. A search was conducted in PubMed and Scopus, initially yielding 502 articles published since 2010 on the topic using search terms related to ICU patients, muscle and physical outcomes, and nutrition delivery. Articles were screened based on inclusion criteria, resulting in 45 articles included in the analysis. Findings indicated that the outcome domains used ranged from muscle strength, muscle mass, to function. Imaging techniques and performance-based measures were the most used type of tools, with varying comprehensiveness, precision, simplicity, and feasibility. Despite most studies using repeated measurements throughout ICU stays, challenges in performing comprehensive assessments were reported. This review provides an overview of the assessment tools utilized in ICU nutritional research, highlighting the variability of choice that can be suited with researcher’s objectives and the availability of resources. To improve consistency and comparability across studies, future research should focus on developing standardized protocols for selecting appropriate tools to measure the effects of nutrition delivery on muscle and physical-related outcomes.
Pengaruh E-Trust dan E-Service Quality Terhadap E-Loyalty Pengguna Layanan Mobile Banking Bank Umum Syariah
Authors: Nengsih Novia , Septianna Syifa 
Published: February 2025

This research aim to determine the influence of e-trust and e-service quality on the e-loyalty of mobile banking users at Islamic commercial banks. This research employs a quantitative research method. The sampling technique used is purposive sampling. The data analysis techniques employed include testing the research instrument, classical assumption tests, multiple linear regression analysis, t-tests, F-tests, and the R² test. The results of the study indicate that e-trust has a significant influence on the e-loyalty of mobile banking users at Islamic commercial banks. Furthermore, e-service quality also has a significant influence on the e-loyalty of mobile banking users at Islamic commercial banks. Moreover, e-trust and e-service quality together have a significant effect on e-loyalty.. Based on the results of the coefficient of determination (R Square) test, a value of 0.505 or 50.5% was obtained. This indicates that e-trust and e-service quality together explain 50.5% of the variability in e-loyalty of mobile banking users at Islamic commercial banks, while the remaining 49.5% is influenced by other variables not included in this study.
Propuesta de transformación hacia la Industria 4.0 del monitoreo, inspección y mantenimiento de tanques reservorios de crudo en Ecuador
Authors: Ocaña Pañora Lando Stephen , Chiza Llambo Juan Carlos , Tibanquiza Chuncho Santiago Efrain , Moreno Garzón Geovanny Patricio 
Published: February 2025

Introducción. La industria petrolera en Ecuador debe transicional la forma de monitoreo, inspección y mantenimiento de tanques de crudo. Mediante la Industria 4.0, con IoT, IA y sensores inteligentes, se mejora eficiencia y reduce costos. No obstante, existen brechas tecnológicas y económicas de implementación. Se propone un modelo para modernizar y optimizar estos procesos. Objetivo. Plantear los requerimientos técnicos y tecnológicos necesarios para la evolución de los procesos de monitoreo, inspección y mantenimiento en los tanques de crudo en la industria petrolera en Ecuador en Ecuador hacia un modelo basado en los principios de la Industria 4.0, con el fin de optimizar la eficiencia operativa, reducir riesgos ambientales y mejorar la sostenibilidad. Metodología. El modernizar el monitoreo, inspección y mantenimiento de tanques de crudo hacia Industria 4.0 con IoT, drones y sistemas ciberfísicos normados incluye análisis diagnóstico, definición de requisitos, diseño tecnológico, pruebas piloto y evaluación de impacto. Resultados. El sistema físico y ciberfísico de la Industria 4.0 integra sensores IoT, robots, drones y tecnologías avanzadas. Estimado una inversión de $196,000, con la que se optimiza eficiencia, seguridad y costos operativos, dentro de estándares globales y garantizando sostenibilidad a largo plazo. Conclusión. La implementación de tecnologías de la Industria 4.0 para el monitoreo, inspección y mantenimiento de tanques reservorios de crudo es factible en industrias petroleras de Ecuador, ya que, con una inversión inicial de $196,000, se podría optimizan costos, aumentan la seguridad en operarios y ofrecer un alto retorno de inversión a largo plazo. Área de estudio general: Industria y tecnología. Área de estudio específica: Industria 4.0, Oil & Gas. Tipo de estudio: Artículo original.
Dysregulated biosynthesis and hydrolysis of cyclic-di-adenosine monophosphate impedes sporulation and butanol and acetone production in Clostridium beijerinckii NCIMB 8052
Authors: Awaga-Cromwell Marian M. , Kumar Santosh , Truong Hieu M. , Agyeman-Duah Eric , Okonkwo Christopher C. , Ujor Victor C. 
Published: February 2025

IntroductionAlthough solventogenic Clostridium species (SCS) produce butanol, achieving high enough titers to warrant commercialization of biobutanol remains elusive. Thus, deepening our understanding of the intricate cellular wiring of SCS is crucial to unearthing new targets and strategies for engineering novel strains capable of producing and tolerating greater concentrations of butanol.MethodsThis study investigated the potential role of cyclic-di-adenosine monophosphate (c-di-AMP) in regulating solvent biosynthesis in C. beijerinckii NCIMB 8052. Genes for c-di-AMP-producing and degrading enzymes [DNA integrity scanning protein A (disA) and phosphodiesterase (pde), respectively] were cloned in this organism and the recombinant strains were characterized relative to the control strain.ResultsPlasmid-borne expression of disA in C. beijerinckii led to a 1.83-fold increase in c-di-AMP levels and near complete (∼100%) inhibition of butanol and acetone biosynthesis. Conversely, c-di-AMP concentrations in the pde-expressing strain reduced 7.54-fold relative to the control with 4.20- and 2.3-fold reductions in butanol and acetone concentrations, respectively, when compared to the control strain. Relative to the control and the pde-expressing strains, the disA-expressing strain produced 1.50- and 1.90-fold more ethanol, respectively. Enzyme activity assays show that core solvent biosynthesis enzymes are mostly inhibited in vitro by exogenously supplemented c-di-AMP (50 nM). Both recombinant strains of C. beijerinckii are impaired for sporulation, particularly the disA-expressing strain.DiscussionCollectively, the results show that dysregulated production and hydrolysis of c-di-AMP severely impair butanol and acetone biosynthesis in C. beijerinckii, suggesting broader roles of this second messenger in the regulation of solventogenesis and likely, sporulation in this organism.
Perclose ProGlide closure devices vs. surgical removal for veno-arterial extracorporeal membrane oxygenation decannulation: a meta-analysis
Authors: Zhang Qian , Guo Ke , Liu Yunping , Wei Wei , Zhao Kan , Huang Haijun , Yao Zuoyi 
Published: February 2025

ObjectivePerclose ProGlide closure devices (PPCDs) have become a more commonly used strategy in veno-arterial extracorporeal membrane oxygenation (VA-ECMO) decannulation, but there is still uncertainty regarding their efficacy and safety compared to surgical removal (SR). Therefore, we conducted a meta-analysis to compare the application results of the two methods in VA-ECMO decannulation.MethodsData from PubMed, Cochrane Library, and EMBASE databases were systematically searched through May 2024. Prospective or retrospective studies on the comparison of PPCDs and SR in VA-ECMO decannulation were included. The outcomes included technical success, bleeding events, infections at the decannulation site, vascular complications, overall complications, mortality and duration of hospitalisation.ResultsEight retrospective studies involving 618 patients were included. The results demonstrated that PPCDs significantly reduced infections at the decannulation site and overall complications [odds ratio (OR) = 0.14, 95% confidence interval (CI) 0.05–0.44, P &lt; 0.001], (OR = 0.27, 95% CI 0.16–0.48, P &lt; 0.001). No significant differences were observed in the incidence rates of bleeding events, vascular complications, mortality and duration of hospitalisation between the two groups (P &gt; 0.05). Subgroup analysis revealed that the SR group had a significantly higher risk of the removal site infection compared to the percutaneous pre-closure group (OR = 0.06, 95% CI 0.01–0.29, P = 0.0003).ConclusionPre-closure techniques utilizing PPCDs demonstrate a significant advantage over SR in reducing the overall incidence of complications for VA-ECMO decannulation, particularly in terms of reducing infections at the decannulation site.
Instant termination as a novel indicator for prognosis of persistent atrial fibrillation during cryoballoon ablation: a propensity score-matched analysis
Authors: Fan Youqi , Ye Jian , Wang Xiaoya , Song Liuguang , Zhang Yu , Wang Yaping 
Published: February 2025

BackgroundInstant atrial fibrillation termination (AFT) during radiofrequency ablation has been suggested as a predictor of prognosis in persistent atrial fibrillation (AF). However, its role in cryoballoon ablation remains unclear. This study investigated the association between AFT and recurrent atrial tachyarrhythmia in patients with persistent AF undergoing cryoballoon ablation.MethodsPatients with non-valvular, drug-resistant, persistent AF who underwent cryoballoon ablation between January 2021 and June 2023 were included and categorized based on the presence or absence of AFT. Propensity score matching (PSM) was applied to eliminate covariate imbalances. Baseline characteristics, procedural details, and clinical outcomes were compared between the groups.ResultsA total of 189 patients [65.0 (59.0–71.0) years] were included. Among them, 41 experienced instant AFT, while 148 remained in AF rhythm. The baseline conditions were similar, except that patients with AFT presented significantly lower left atrial diameter (LAD). During a follow-up of 16.0 [9.1–26.9] months, the recurrence rates of arrhythmias were significantly lower in the AFT group (log-rank P = 0.044). Both AFT [HR: 0.298, 95% CI: (0.091–0.976), P = 0.035] and baseline LAD [HR: 1.079, 95% CI: (1.012–1.151), P = 0.021] were independent predictors of recurrence. We further assessed the prognostic value of AFT in PSM groups which showed that the recurrence rates were also significantly lower in the AFT group (log-rank P = 0.049).ConclusionInstant AFT during cryoballoon ablation is associated with a reduced risk of arrhythmic recurrence in patients with persistent AF.
The crucial role of hypertension in determining latent classes of metabolic syndrome in northern Iran and predictive power of these classes in non-alcoholic fatty liver: a gender-based insight
Authors: Amirkalali Bahareh , Hassanzadeh Parvin , Sheikholmolooki Fatemeh , Gholizadeh Esmaeel , Doustmohammadian Azam , Safarnezhad Tameshkel Fahimeh , Motamed Nima , Maadi Mansooreh , Sohrabi Masoudreza , Sobhrakhshankhah Elham , Zamani Farhad , Ajdarkosh Hossein 
Published: February 2025

IntroductionThis study investigates the subclasses of metabolic syndrome (Mets) and their relationship with non-alcoholic fatty liver (NAFLD) and the probable predictive role of serum vitamin D and CRP levels.MethodsThis community-based, cross-sectional study was performed on adults in the framework of the Amol cohort prospective study (AmolCPS). Mets was defined as Adult Treatment Panel III criteria (ATP III) and ultrasound was used to diagnose NAFLD. Anthropometric and blood pressure measurements were conducted, and biochemical measurements were assessed after fasting. Data analysis included Latent class analysis, two-tailed χ2 statistics, one-way analysis of variance, and logistic regression using Mplus (version 7.4) and spss (version 26) softwares.ResultsThe study involved 2308 participants, with a mean age of 43.17 ± 12.30 years. Mets prevalence was 25.64%, with three identified classes: Mets with Hypertension (HTN), Mets without HTN (Non-HTN), and Low Risk. Mets with HTN had a high probability of at least four components, particularly high SBP. Non-HTN had at least three high probable components, especially high TG and low HDL but not high SBP and DBP. The low-risk class had a low probability of all components except low HDL in women. Serum vitamin D and CRP levels did not significantly predict Mets classes in men, while CRP level significantly predicted the HTN class in women (OR:1.03, CI:1.004-1.067). Both HTN, and Non-HTN Mets classes significantly increased the odds of NAFLD compared to the low risk class, especially in women (HTN class OR: 4.20 vs 2.94; non-HTN class OR: 5.60 vs 3.12 in women and men respectively).ConclusionThe latent class analysis in northern Iran identified three Mets classes: HTN, Non-HTN, and low-risk, with hypertension playing a crucial role in determining these classes. These classes were stronger predictors of NAFLD in women. Serum CRP and vitamin D levels did not emerge as significant predictors of the classes, except for serum CRP in the HTN class among women.
Can whole genome sequencing resolve taxonomic ambiguities in fungi? The case study of Colletotrichum associated with ferns
Authors: Menicucci Andrea , Iacono Salvatore , Ramos Madalena , Fiorenzani Chiara , Peres Natalia Aparecida , Timmer Lavern Wayne , Prodi Antonio , Baroncelli Riccardo 
Published: February 2025

IntroductionThe genus Colletotrichum comprises numerous fungal species with diverse ecological roles, including plant pathogenic, endophytic, and saprophytic lifestyles. Accurate species identification is crucial for understanding host-pathogen interactions, disease epidemiology, and fungal ecology. However, taxonomic classification within Colletotrichum remains challenging due to genetic complexity and phenotypic plasticity. Conventional approaches such as single-gene analyses and multilocus sequence typing (MLST) frequently fail to resolve closely related taxa, leading to misidentifications that hinder species delimitation and comparative evolutionary studies. Whole-genome sequencing (WGS) offers a promising alternative by providing genome-wide resolution for phylogenetic analysis. This study investigates Colletotrichum isolates associated with the fern Rumohra adiantiformis and evaluates the effectiveness of WGS in addressing taxonomic uncertainties.MethodsA total of 18 Colletotrichum isolates, including fern-associated strains, were analyzed. Genomic DNA was extracted and sequenced using the Illumina NovaSeq platform. High-quality genome assemblies were generated, and gene prediction was conducted using AUGUSTUS. Orthogroup assignment and phylogenomic analysis were performed based on single-copy orthologs, and phylogenetic trees were constructed using MLST and WGS-based approaches. Comparative analyses were carried out to assess the taxonomic resolution provided by WGS in relation to traditional methods.ResultsGenome-wide phylogenomic analysis revealed distinct evolutionary lineages among Colletotrichum isolates that MLST failed to resolve, highlighting host and geographic differentiation. High-quality genome assemblies were obtained, with 98.3% of genes assigned to orthogroups, indicating strong genomic conservation. Phylogenetic analyses confirmed a close relationship between Rumohra adiantiformis-associated isolates and Colletotrichum filicis, reinforcing the effectiveness of WGS in species identification.DiscussionThese findings demonstrate the superiority of WGS over MLST in resolving species boundaries and reconstructing evolutionary relationships. The enhanced resolution provided by genome-wide data enables more accurate taxonomic classification, reducing misidentifications and improving our understanding of fungal biodiversity. By refining Colletotrichum taxonomy, WGS facilitates ecological and pathogenic studies, offering a robust framework for future research in fungal systematics and plant pathology. As sequencing technologies continue to advance, WGS is expected to become a standard tool for fungal species delineation and evolutionary studies.
Modulation and distribution of extracellular free water and tract deficits in rhesus macaques before and after the initiation of emtricitabine + tenofovir disoproxil fumarate + dotutegravir treatment
Authors: Nguchu Benedictor Alexander , Lu Yu , Han Yifei , Wang Yanming , Liu Jiaojiao , Li Hongjun , Shaw Peter 
Published: February 2025

IntroductionUnderstanding the specific timing of cART initiation, its effectiveness, and failures, as well as assessing how well the current cART regimens control viral replication and rebound, enhance immune function, and repair or curb early injury in the central nervous system (CNS), is crucial to improving the livelihood of people living with HIV.MethodsHere, we use an animal model to provide controlled environments to understand how the bodies of Chinese-origin rhesus monkeys, both the immune system and CNS, respond to a combination of emtricitabine (EMTBL/FTC), dolutegravir (DTG), and tenofovir disoproxil fumarate (TDF) following the induction of Simian Immunodeficiency Virus (SIV). We injected the rhesus monkeys with a dose of SIVmac239 (i.e., TCID50—a 50-fold half-tissue culture infective dose) through brachial veins and conducted seven follow-ups at baseline, day 10, day 35, day 84, day 168, day 252, and day 336 for MRI imaging and blood/CSF assays of SIV copies and immunity levels.Results and discussionOur experimental data demonstrate that the immune system is compromised as early as 7 days after infection, with a rapid rise of SIV copies in ml and a significant drop of CD4/CD8 ratio below ~1 within the first 14 days of infection. The alterations in the extracellular environments, manifesting as increased free water volume fraction (FW-VF) in MRI data and changes in the diffusivity properties of fiber tissues appearing in FW-corrected FA and FW-corrected MD, occur in parallel with an compromised immune system, suggesting that SIV enters the brain parenchyma in the early days of infection via a weakened brain defense system, causing inflammatory processes affecting the CNS. Our findings demonstrate that our current FTC+TDF+DTG regimen can enhance the immune system, suppress SIV replication, and slow damage to the intra- and extracellular environments. However, it is still ineffective in controlling viral rebound and experiences resistance in some rhesus monkeys, which may lead to further damage to the CNS. Our findings also provide the first SIVmac239-based evidence that extracellular FW-VF may be a more reliable biomarker of abnormal inflammatory processes, thus providing a better understanding of SIV disease progression than previously anticipated.
Associations of magnesium depletion score with the incidence and mortality of osteoarthritis: a nationwide study
Authors: Ma Ruicong , Zhang Cheng , Liu Jiaqing , Ren Jinyi , Huang Huina , Wang Guan , Ding Yanchun , Li Xia 
Published: February 2025

BackgroundMagnesium is an essential immune nutrient for the body, and recent studies have found that it plays an important role in osteoarthritis (OA). Magnesium depletion score(MDS) is a new method for evaluating the magnesium status of the body. Our objective is to explore the association between MDS and the incidence of OA, as well as the relationship between MDS and mortality in patients with OA.MethodsEligible participants were obtained from NHANES from 2005 to 2018. Logistic regression models were employed to evaluate the link between MDS and the incidence of OA. Cox regression models were employed to evaluate the link between MDS and mortality among OA patients. In addition, restricted cubic spline was utilized to explore the correlation between MDS and the incidence of OA, as well as the relationship between MDS and mortality in patients with OA. Subgroup analysis were adopted in order to ensure the credibility of the results in different subgroups, including age, gender, race, education level, BMI, smoking, diabetes and hypertension.Results19,394 individuals qualified for analysis, including 3,256 OA patients. After excluding missing follow-up data, 630 all-cause deaths and 172 cardiovascular deaths (CVDs) were observed in 3,250 OA patients. The individuals with OA had higher levels of MDS. In the logistic regression model, MDS was positively related to OA (MDS≥3 vs. MDS=0, OR =1.83 (1.46-2.30, P&lt;0.001)). Besides, a positive association was observed between MDS and all-cause mortality [MDS≥3 vs. MDS=0, HR =2.56 (1.49-4.41, P&lt;0.001)] and CVDs [MDS≥3 vs. MDS=0, HR =3.00 (1.13-7.98, P=0.01)] in cox regression models. In addition, a 1-unit rise in MDS was significantly linked to an increased risk of mortality. Restricted cubic spline indicated a positive relationship between MDS and incidence and mortality of OA. Subgroup analysis demonstrated that the results are stable in different subgroups.ConclusionsMDS is positively correlated with the incidence and mortality of OA. Optimizing the nutritional status of magnesium may bring benefits to OA patients.
Single-cell transcriptome sequencing reveals the cell populations and functions associated with stony coral Pocillopora damicornis
Authors: Diao Wenguang , Zhang Shengyao , Zhu Xueying , Wu Peiqi , Du Bingyan , Han Zhe , Liu Yunqing , He Chunpeng , Lu Zuhong 
Published: February 2025

IntroductionPocillopora damicornis, a key species of stony corals, has been the subject of considerable scientific study. However, the cellular composition of P. damicornis and the roles of these cells in endosymbiosis and biomineralization remain elusive. The development of single-cell technology has provided new opportunities for researching the cellular and molecular mechanisms underlying symbiosis and mineralization. Nevertheless, the stringent environmental requirements, the complexity of the cellular components, and the paucity of high-quality reference genomes of P. damicornis have posed significant challenges for single-cell transcriptome research.MethodsIn this study, we quantified the transcriptomic expression of P. damicornis by aligning its single-cell transcriptome (scRNA-seq) data to multiple species, including Stylophora pistillata, P. damicornis, and Pocillopora verrucosa. We determined the cell types of P. damicornis by comparing its cluster-specific genes with the published cell type-specific genes of S.pistillata and conducted gene function and enrichment analyses.ResultsUnsupervised clustering analysis yielded the identification of ten distinct cell populations, including epidermis cells, gastrodermis cells, algae-hosting cells, calicoblast, cnidocytes, and immune cells. In addition, we identified 53 genes that were highly similar to known sequences in the symbiotic zooxanthellae. These genes were mainly expressed in four different cell populations, corresponding to active symbiotic populations.ConclusionThis study identified cell types closely associated with symbiosis and calcification in P. damicornis, along with their marker genes, which are consistent with the findings in S. pistillata. These results offer insights into the cellular functions and symbiotic mechanisms of P. damicornis.