bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of PFKFB3 Promotes Cell Glycolysis and Proliferation in Renal Cell Carcinoma
Authors: Li Jun , Zhang Shiqiang , Liao Dingzhun , Zhang Qian , Chen Chujie , Yang Xiangwei , Jiang Donggen , Pang Jun 
Published: March 2021

Abstract
Background: Cancer cells prefer aerobic glycolysis to increase their biomass and sustain uncontrolled proliferation. As a key glycolytic activator, phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) has been implicated in the progression of multiple types of tumors. However, the specific function and clinical significance of PFKFB3 in renal cell carcinoma (RCC) remain unclear. In the present study, we explored the role of PFKFB3 in RCC.Methods: We analyzed the expression of PFKFB3 in clear cell renal cell carcinoma (ccRCC) tissues and its relationship with clinical characteristics of ccRCC. Real-time PCR and Western blot analysis were used to detect PFKFB3 expression levels in different RCC cell lines. Furthermore, we determined the glycolytic activity by glucose uptake, lactate secretion assay and ECAR analysis. CCK-8 assay, clone formation assay, flow cytometry and EdU assay were performed to monitor cancer cell proliferation and cell cycle distribution. In addition, nude mice xenograft model was used to investigate the role of PFKFB3 in tumor growth in vivo.Results: In this study, we found that PFKFB3 was significantly up-regulated in RCC tissues and cell lines compared with normal control. Overexpression of PFKFB3 was positively associated with advanced TNM stage and could predict poor prognosis of ccRCC patients. Furthermore, knockdown of PFKFB3 suppresses cell glycolysis, proliferation and cell cycle G1/S transition in RCC cells. Importantly, in vivo experiments confirmed that PFKFB3 knockdown delayed tumor growth derived from the ACHN cell line.Conclusion: Our results suggest that PFKFB3 plays an important role in the progression of RCC via mediating glycolysis and proliferation, and provides a potential therapeutic target for RCC.
Exploring the role of exposure to green space in preventing anxiety and depression among young people aged 14-24 living in urban settings: a systematic review
Authors: Bray Isabelle , Reece Rebecca , Sinnett Danielle , Martin Faith , Hayward Robert 
Published: March 2021

Abstract
Background Despite the growing problem of anxiety and depression amongst young people aged 14-24 years living in urban settings, reviews about the role of exposure to green space or nature in preventing anxiety and depression tend to focus on children, adults or sometimes adolescents. This review aims to explore whether, and if so how, exposure to green space reduces the risk of anxiety and depression among young people aged 14-24 living in urban settings.MethodsCINAHL plus, Global Health, MEDLINE, ProQuest: Dissertations and Theses, PsycINFO, Scopus and OpenGrey were searched for research published in English between January 2000 and June 2020, including all study designs. Results 9,208 sources were identified and 48 were included. Experimental studies provided evidence that walking or being in a green space improves mood and state anxiety immediately following the intervention. Observational studies suggest that social interaction, physical activity, and mindfulness mediate the relationship between exposure to green space and mental health. We propose that the absence of noise and traffic and the presence of nature promotes mindfulness and restoration.ConclusionsThis review provides evidence to clinicians about the value of green social prescribing, and to policymakers about the value of bringing the benefits of forests, vegetation and nature into cities, and ensuring that these spaces are accessible and safe for young people to use.
Carotid Disease and Retinal Optical Coherence Tomography Angiography Parameters in Patients with Non-arteritic Anterior İschemic Optic Neuropathy
Authors: KAYA Fatma Selin 
Published: March 2021

Abstract
Purpose: To analyze the data and retinal microvasculature for nonarteritic anterior ischemic optic neuropathy (NAİON) patients referred to have carotid Doppler ultrasound from 2016 to 2020. Methods: In this case-control observational study, 30 NAİON patients evaulated with carotid doppler sonography. 22 NAION patients (at least 3 months after onset of symptoms) and 9 normal subjects underwent a complete ophthalmic examination including optical coherence tomography (OCT), optical coherence tomography angiography (OCT-A). NAİON eyes and fellow eyes were further divided into the groups according to presence of carotid stenosis (CS). NAION patients with CS were termed "CS-NAION"; those without were termed "NCS-NAION". Measurements of radial peripapillary capillary vessel density (RPC VD) and ganglion cell complex (GCC), disk retinal nerve fiber layer (RNFL) thickness were compared among groups. Results: Fourteen of 30 NAİON patients referred for carotid Doppler were positive for CS with all of such referrals having less than 50% stenosis. RNLF, GCC and RPC VDs were reduced in NAION eyes, when compared to controls and the fellow eyes. RPC VD was significantly lower in the temporal-superior (P=0.037) and the superior-temporal (P=0.012) sectors of the NCS-NAION patients than in the CS-NAION patients. No significant differences were found between CS-fellow eyes and NCS-fellow eyes in terms of RPC VDs, RNLF or GCC. Conclusion: Results of the study highlight the effect of carotid artery stenosis on ocular perfusion pressure in the pathogenesis of NAİON. More extensive studies are necessary.
Left Breast Radiotherapy With Multi Fields Hybrid Intensity Modulated Radiotherapy (IMRT) Versus Volumetric Modulated Arc Therapy (VMAT): Balance Between Left Anterior Descending Artery Sparing and Secondary Cancer Induction Risk
Authors: Xiang Xiaoyong , Ding Zhen , Kang Kailian , Dai Zhitao , Zhang Wenjue , Zeng Qi , Xu Meiling , Yuan Qingqing 
Published: March 2021

Abstract
To explore the feasibility of using Volumetric-Modulated Arc Therapy (VMAT) to protect left anterior descending branch (LAD) after breast-conserving surgery for left breast cancer. 15 left breast cancer patients after breast-conserving surgery were selected. 7F-IMRT and 2A-VMAT treatment plans were designed with Varian Eclipse TPS (13.6version). The prescriptions of PTV and PTV Boost were 43.5Gy and 49.5Gy in 15 fractions. The dosimetric parameters, OARs dose sparing and second cancer risk (SCR) were compared between the two plans using a paired t-test. The VMAT plans obtain better PTV conformity and higher mean dose. VMAT plans show a better dose distribution in high dose areas and better sparing of OARs, including left lung, heart, and LAD. The Dmax and Dmean of LAD decreased significantly in VMAT plans. The SCRs of the contralateral lung and breast significantly increased with a higher mean dose. We recommend that contouring and evaluating the dose of LAD and LAD helping structures in left breast cancer radiotherapy. SCR should be evaluated for younger patients.
Simulation and Experimental Study on Improving Electrochemical Machining Stability of High Convex Structure on Casing Surface by Using Backwater Pressure
Authors: Ge Zhenghui , Chen Wangwang , Zhu Yongwei 
Published: March 2021

Abstract
Casing parts are regarded as one of the key components in aero-engine components. Most casing parts are attached with different shapes of convex structures, and their heights range from hundreds of microns to tens of millimeters. The use of profiling blocky electrodes for electrochemical machining of casing parts is a widely used method, especially in the processing of high convex structures. However, with the increase of convex structure height, the flow field of machining areas will become more complex, and short circuits may occur at any time. In this study, a method to improve the flow field characteristics of machining area by adjusting the backwater pressure is proposed, the simulation and experiment are carried out respectively. The simulation results showed that the back-pressure mehtod can significantly improve the uniformity of the flow field around the convex structure compared with the extraction outlet mode and the open outlet mode, and then the optimized back-pressure of 0.5 MPa was obtained according to simulation results. The experimental results showed that under condition of the optimized back-pressure parameters, the cathode feed-rate increased from 0.6 mm/min to 0.8 mm/min, and the convex structure with a height of 18 mm was successfully machined. This indicated that the back-pressure method is suitable and effective for the electrochemical machining of high convex structure with blocky electrode.
Recycling of Beet Sugar Byproducts and Wastes Enhances Sugar Beet Productivity and Salt Redistribution in Saline Soils
Authors: Aljabri Maha , Alharbi Saif , Ismaeil Fekry M. , Chen Jiana , Abou-Elwafa Salah Fatouh 
Published: March 2021

Abstract
Soil salinity adversely affects the growth, yield, and quality parameters of sugar beet, leading to a reduction in root and sugar yields. Improving the physical and chemical properties of salt-affected soils is essential for sustainable cultivation and sugar beet production. A field experiment was conducted at t the Delta Sugar Company Research Farm, El-Hamool, Kafr El- Sheikh, Egypt to evaluate the response of sugar beet to the application of beet sugar filter cake treated with sulfuric and phosphoric acid-treated, phosphogypsum (PG), desaline, humic acid and molasses under saline soil conditions. The application of treated filter cake enhanced root length, diameter and leaf area. The application of molasses enhanced root length, diameter and leaf area as well. Application of molasses increased sugar content and root yield. The application of either treated filter cake or molasses produced the highest recoverable sugar yield. Linear regression analysis revealed that the root yield, quality index and recoverable sugar yield increased in response to the increased availability of either Ca2+ and K content in the soil which increases in response to the application of soil amendments and molasses. The application of treated beet sugar filter cake and molasses increased the calcium, magnesium and potassium availability in the soil. Treated filter cake is a promising organic soil amendment that enhanced the yield by 29%, and yield-related traits of sugar beet by improving the physical and chemical properties of the soil.
Translation, adaptation and validation of the Bulgarian version of the King’s Parkinson’s Disease Pain Scale
Authors: Stoyanova-Piroth Galina , Milanov Ivan , Stambolieva Katerina 
Published: March 2021

Abstract
Introduction:The purpose of the present study was to translate and cross-culturally adapt the King's Parkinson's Disease Pain Scale (KPPS) into Bulgarian and to investigate its psychometric properties in order to provide a validated Parkinson’s disease-specific pain instrument in Bulgarian language (KPPS-BG).Methods:Translation into Bulgarian and a cultural adaptation were performed to obtain KPPS-BG. A total of 162 patients with idiopathic Parkinson’s disease were screened for pain using the complementary to the KPPS questionnaire-King’s Parkinson's Disease Pain Questionnaire (KPPQ). KPPS-BG domain and total scores were calculated and internal consistency, construct validity and test-retest reliability were examined for 129 patients having one or more positive items in the KPPQ-BG.Results:79.6% of the patients reported one or more types of pain. The most common type was musculoskeletal pain (83.7%), followed by nocturnal pain (55.0%), fluctuation-related pain (50.1%), radicular pain (43.4%), chronic pain (31.0%), discoloration, edema/swelling (27.1%) and, oro-facial pain (14.3%). Mean KPPS-BG total score was 21.1±17.3 SD. KPPS-BG showed a good reliability (Cronbach's alpha 0.75). The test-retest reliability of the KPPS-BG was high and the intraclass correlation coefficient was 0.92, demonstrating а good repeatability. KPPS-BG total score was higher in patients with postural instability gait difficulty motor subtype, compared to tremor-dominant or indeterminate subtype. Significant positive correlations were found between KPPS-BG total score and modified H&Y, Movement Disorders Society Unified Parkinson’s Disease Rating Scale part III.Conclusion:The KPPS-BG constitutes a reliable, comprehensive and useful tool for pain assessment in native Bulgarian patients with Parkinson’s disease.
MIBG Cardiac Scintigraphy as a Potential Biomarker That Reflects Severity in Idiopathic REM&nbsp;Sleep Behavior Disorder
Authors: You Sooyeoun , Won Kyoung Sook , Kim Keun Tae , Lee Hyang Woon , Cho Yong Won 
Published: March 2021

Abstract
Study Objectives: 123I-metaiodobenzylguanidine (MIBG) cardiac scintigraphy was performed to demonstrate the correlation with clinical characteristics of patients with idiopathic REM sleep behavior disorder (iRBD) and we found the factors that are associated with neurodegenerative diseases. Methods: All subjects including 39 patients with iRBD and 17 healthy controls underwent MIBG cardiac scintigraphy to assess cardiac autonomic dysfunction. The iRBDs were confirmed by in-lab overnight polysomnography. Receiver operating curve was performed to determine cut-off value of early and delayed heart to mediastinum ratio (HMR) in patients with iRBD. Based on each cut-off value, comparison analysis about RWA was performed by dividing into two groups within iRBD patients.Results: MIBG uptake below the cut-off value is associated with higher REM sleep without atonia (RWA). The lower HMR had significantly higher RWA (%) both with the cut-off value of early (11.0±5.6 VS 29.3±23.2, p=0.018) and delayed HMR (9.1±4.3 VS 30.0±22.9, p=0.011).Conclusion: This study suggests that MIBG uptake is a potential biomarker of the severity of RWA in iRBD.
Association of Biomarkers Related to Preoperative Inflammatory and Coagulation with Postoperative In-Hospital Deaths in Patients with Type A Acute Aortic Dissection
Authors: Li Ming , Sun Haifeng , Xu Suochun , Yan Yang , Wang Haichen , Zheng Jianjie , Li Yongxin , Zhang Yongjian , Hao Junjun , Deng Chao , Zheng Xinglong , Liu Miaomiao , Gao Yang , Wang Xue , Xue Li 
Published: March 2021

Abstract
Background: The aim of this study was to analyze the predictive value of biomarkers related to preoperative inflammatory and coagulation in the prognosis of patients with type A acute aortic dissection (AAD). Methods: A total of 206 patients with type A AAD who had received surgical treatment were enrolled. Patients were divided into two groups according to whether they died during hospitalization. Peripheral blood samples were collected before anesthesia induction. Preoperative levels of D-dimer, fibrinogen (FIB), platelet (PLT), white blood cells (WBC) and neutrophil (NEU) between the two groups were compared. Univariate and multivariate logistic regression analysis were utilized to identify the independent risk factors for postoperative in-hospital deaths of patients with type A AAD. Receiver operating characteristic (ROC) curve were used to analyze the predictive value of D-dimer, FIB, PLT, WBC, NEU and CRP in the prognosis of the patients. Results: Univariate logistic regression analysis showed that the P values of the five parameters including D-dimer, FIB, PLT, WBC and NEU were all less than 0.1, which may be risk factors for postoperative in-hospital deaths of patients with type A AAD. Further multivariate logistic regression analysis indicated that higher preoperative D-dimer and WBC levels were independent risk factors for in-hospital deaths of patients with type A AAD. ROC curve analysis indicated that FIB+PLT combination is provided with the highest predictive value for in-hospital deaths.Conclusion: Both preoperative D-dimer and WBC in patients with type A AAD may be used as independent risk factors for the prognosis of such patients. Combined use of FIB and PLT may improve the accuracy and accessibility of clinical prognostic assessment.
Predictive Utility of Changes in Optic Nerve Sheath Diameter After Cardiac Arrest for Neurologic Outcomes
Authors: Lee Heekyung , Lee Joonkee , Shin Hyungoo 
Published: March 2021

Abstract

Background: Optic nerve sheath diameter (ONSD) is effective in predicting the neurologic outcome of patients with post-cardiac arrest (CA) return of spontaneous circulation. This study aimed to investigate the effect of ONSD changes before and after CA on neurologic outcomes in patients with return of spontaneous circulation after CA using brain computed tomography (CT).Methods: We included patients who were hospitalized after CA and underwent pre- and post-CA brain CT from January 2001 to September 2020. They were divided into the good and poor neurologic outcome (GNO and PNO, respectively) groups based on the neurologic outcome at hospital discharge. We performed between-group comparisons of the amount and rate of post-CA changes on brain CT. Moreover, we calculated the area under the curve to determine the predictive value of ONSD changes for neurologic outcomes.Results: We included 96 enrolled patients; among them, 25 had GNO. The amount of change was significantly higher in the PNO group than in the GNO group (0.63 vs. 0.30 mm; p = 0.030). Moreover, there was a higher rate of change in the PNO group than in the GNO group (12.29 vs. 5.26 %; p = 0.041). The AUC for predicting PNO was 0.64 (95% CI = 0.53–0.73; p = 0.04) and patients with a rate of ONSD change > 27.2% had PNO with specificity and positive predictive values of 100%.Conclusion: The amount and rate of ONSD changes on brain CT were significantly associated with the neurologic outcomes in post-CA patients. ONSD changes may predict neurologic outcomes in post-CA patients.
Knowledge-Practice Gap Regarding Surgical Scrubs Among Medical Students: A Cross-Sectional Survey
Authors: Shahzad Hania , Fraz Khan Osama Bin , Siddiqui Ayat , Rizvi Narjis 
Published: March 2021

Abstract
Background: Medical students are involved in various aspects of patient care. Their lack of knowledge regarding infection control can compromise the safety of the patients. In this study, we describe the current knowledge and practice of medical students regarding surgical scrubs.Methods: We conducted a cross-sectional survey among medical students using a self-administered questionnaire. The questions were based on the American Association of Surgical Technologists Standards of Practice for Surgical Attire, Surgical Scrub, Hand Hygiene, and Handwashing.Results: Most students correctly identified the components of surgical scrubs including scrub suit (95.5%), shoe covers (95.4%), headcover (94.9%), and masks (93.9%). 82.7% of students were aware of the policy of wearing surgical scrubs off-campus, 78.7% knew the correct method of using the protective over gowns, only 52.8% knew permissible areas for wearing surgical scrubs. Students’ adequate knowledge did not translate into their practices. 57.1% of the students reported not having received any formal education on scrub use. Inconvenience (66.9%), was cited as the most common cause of scrub re-use and unsafe practices.Conclusions: Medical students have sufficient knowledge regarding surgical scrubs which does not translate into their practices. The majority of the students report as not having received any formal training regarding surgical scrub guidelines. Improving medical student surgical scrub practices may help reduce the burden of HAIs in developing countries.
Optimization and Application of Loop-mediated Isothermal Amplification Detection Method on Little Cherry Virus 1
Authors: Wang Deya , Li Zhili , Yang Weikang , Gao Chenmeng , Cao Xinran , Deng Yanmei 
Published: March 2021

Abstract
Background: Little cherry virus 1 (LChV-1) is one of the most pathogenic virus that frequently infect cherry and have caused serious economic damage to production and quality of cherry. But current detection methods of LChV-1 was less.Objective: This study aims to optimization of loop-mediated isothermal amplification (LAMP) detection method on Little cherry virus 1, and lies the foundation for the application of LChV-1 fast diagnosis in the field in Shandong province.Methods: We performed one-step RT-LAMP at temperature of 59℃ for 60 minutes, whereas the reaction system contained 5.2 mM Mg2+, 8 U Bst 2.0 DNA polymerase, 1.4 mM deoxyribonucleotide and 0.2 and 1.6 µM of the outer and inner primers, respectively. And,we used the RT-LAMP method to detect 65 field samples from Shandong province, and discovered that 5 samples were infected by LChV-1, the detection results were consistent with the RT-PCR results.Conclusions: The RT-LAMP method is highly specific, sensitive, practicable and efficient and suitable method for detection LChV-1 samples.
Congestive Heart Failure Exhibited Higher BMI With Lower Energy Intake and Lower Physical Activity Level: Data From the National Health and Examination Nutrition Survey
Authors: Xu Tianyu , Zhou Haobing , Ma Zhuang , Zhang Hao , Huang Yuli , Zeng Qingchun , Xu Dingli , Zhang Yuhui , Zhang Jian 
Published: March 2021

Abstract
Introduction: Despite nutritional deficiency existed in congestive heart failure (CHF), there is a large amount of CHF patients suffer from obesity. This study aimed to identify the differences for increased BMI or obesity in CHF patients.Methods: This cross-sectional study included adults from the National Health and Nutrition Examination Survey 2007-2016. Differences were compared between CHF participants versus non-CHF participants, and BMI ≥ 30kg/m2 versus BMI < 30kg/m2 CHF participants. Results: CHF participants were with higher BMI, lower energy and macronutrients intakes, lower physical activity level and longer rest time, lower hematocrit and hemoglobin level (all P <0.05) than non-CHF participants. The prevalence of BMI ≥ 30kg/m2 in patients with CHF was 53.48%. There was no significant difference observed in energy, macronutrients intake between CHF patients with BMI ≥ 30kg/m2 or < 30kg/m2. The water intake (P =0.032), sedentary time (P =0.002), and hematocrit (P =0.028) were significantly different between CHF with BMI ≥ 30kg/m2 and with < 30kg/m2.Conclusion: Compared with non-CHF participants, CHF patients exhibited higher BMI with lower energy and macronutrients intakes, lower physical activity level, longer rest time and hemodilution with lower hematocrit and hemoglobin level. Among CHF patients with BMI ≥ 30kg/m2, higher sedentary time and hematocrit were observed.