bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-328a-3p stimulates endothelial cell migration and angiogenesis
Authors: Chen Sailing , zhang Yunsong , Cai Xiaodong , Yi Sheng , Wang Xinghui 
Published: January 2021

Abstract
Background

Endothelial cells play important biological roles after peripheral nerve injury by forming blood vessels within the nerve gap after peripheral nerve injury and guiding Schwann cell migration. microRNAs (miRNAs) affect cellular behavior and regulate a wide variety of physiological and pathological activities, including peripheral nerve regeneration. Emerging studies identified the essential roles of miRNAs on the phenotype modulation of Schwann cells while the effects of miRNAs on endothelial cells were not well investigated. miR-328a-3p was differentially expressed in peripheral nerve stumps after nerve injury. This study aimed to identify the biological effects of miR-328a-3p on human umbilical vein endothelial cells (HUVECs).
Methods

Here, the biological functions of miR-328a-3p on endothelial cells were determined by transfecting cultured human umbilical vein endothelial cells (HUVECs) with miR-328a-3p mimic or inhibitor. Heatmaps of lncRNAs and mRNAs were generated using meV software according to previous obtained sequencing data of sciatic nerve stumps at 0, 1, 4, 7, and 14 days after nerve crush injury.
Results

Transfection with miR-328a-3p mimic led to slightly decreased HUVEC proliferation and robustly increased HUVEC migration and tubulogenesis while transfection with miR-328a-3p mimic led to opposite observations. Bioinformatic analysis further discovered potential regulators and effectors of miR-328a-3p and constructed a miR-328a-3p-centered competing endogenous RNA (ceRNA) network.
Conclusions

Collectively, our present study demonstrated that dysregulated miR-328a-3p after peripheral nerve injury might affect the migration and angiogenesis of endothelial cells and contribute to peripheral nerve regeneration.
Trial registration:

The rats were obtained from the Experimental Animal Center of Nantong University (Animal licenses No. SCXK [Su] 2014-0001 and SYXK [Su] 2012-0031).
Ultrasound feature discordance in a bilateral juvenile ovarian granulosa cell tumor: A case report
Authors: Zhang Li , Cai Sheng , Wang Yahong , Wang Ying , Ge Zhitong , Zou Mi , Li Jianchu , Huang Huifang 
Published: January 2021

Abstract
BackgroundAs a rare ovarian stromal tumor, the juvenile ovarian granulosa cell tumor (JGCT) is mainly seen in premenstrual and young women. It associates with high malignancy and recurrence rate, and early diagnosis and treatment could improve prognosis. Most cases are unilateral solid-cystic or solid masses, while unilocular cystic masses are rarely seen. As cystic ovarian tumors are often misdiagnosed as benign cystic lesions or functional cysts, cystic JGCT should not be overlooked.Case presentationWe report this special case of a 10-year-old female patient. It’s the first reported JGCT with completely inconsistent bilateral imaging manifestations, including an extremely rare unilocular cystic JGCT. A large solid-cystic JGCT appeared in her left ovary, and left adnexal resection was performed. A unilocular cyst occurred in the right ovary two years later. It was with a regular shape and smooth inner wall, mimicking a benign cyst. Since the patient was young and had the desire to preserve the right ovary, follow-up was initially recommended. However, the cyst size increased rapidly and exploratory laparotomy was finally performed. Pathology showed a unilocular cystic JGCT.ConclusionsAs cystic masses in young patients are easily misdiagnosed as benign or physiologic lesions, this case emphasizes the importance of postoperative follow-up for JGCTs. Exploratory laparotomy of persistent cystic lesions should be considered when necessary.
Neutralization of N501Y mutant SARS-CoV-2 by BNT162b2 vaccine-elicited sera
Authors: Shi Pei-Yong , Xie Xuping , Zou Jing , Fontes-Garfias Camila , Xia Hongjie , Swanson Kena , Cutler Mark , Cooper David , Menachery Vineet , Weaver Scott , Dormitzer Philip 
Published: January 2021

Abstract
Rapidly spreading variants of SARS-CoV-2 that have arisen in the United Kingdom and South Africa share the spike N501Y substitution, which is of particular concern because it is located in the viral receptor binding site for cell entry and increases binding to the receptor. We generated isogenic N501 and Y501 SARS-CoV-2. Twenty human sera from the mRNA-based vaccine BNT162b2 trial exhibited equivalent neutralizing titers to the N501 and Y501 viruses.
Effects of Stand Density on Soil Respiration and Soil Labile Organic Carbon and Their Influence Mechanism in Larix Principis-Rupprechtii Plantations
Authors: Liu TaiRui , Peng Daoli , Tan Zhijie , Guo Jinping , Zhang Yunxiang 
Published: January 2021

Abstract
BackgroundForest soil carbon pool plays a vital role in the global carbon sequestration and carbon emission. Forest management can regulate the sequestration and output of forest soil carbon pool to a certain extent, but mechanism of forest density effects on soil carbon pool still needs to be further researched. MethodsWe established sample plots with density gradients in three-age stands of Larix principis-rupprechtii plantation and measured soil respiration (RS), soil organic carbon (SOC), soil dissolved organic carbon (DOC), and microbial biomass carbon (MBC), light fraction organic carbon (LFOC), and easily oxidizeable organic carbon (ROC). Results and ConclusionsThe results showed that, among the forest stands of three ages, RS, heterotrophic respiration (RH), MBC, LFOC, ROC of different stand density levels were significantly different. Moderate density promotes RS rate and RH rate and the sequestration of MBC and LFOC and inhibits ROC sequestration. With the increase of forest stand density, RS, RH, LFOC, and MBC first increased and then decreased, and ROC first decreased and then increased, the quadratic function could fit these changing trends. The RS, RH, and autotrophic respiration (RA) rates of older forest stands were relatively fast, and contents of SOC, MBC, LFOC, DOC, and ROC were higher, and they were more sensitive to changes in stand density. SOC, LFOC, MBC, DOC, and ROC explained 56.05% variations of RS, Rh, and RA. MBC, LFOC, and ROC in soil labile organic carbon were closely related to RS and Rh, but not SOC. Among them, LFOC and MBC played the role of "warehouse" and "tool" and significantly correlated with RS and Rh. ROC, as "raw material," had a significant negative correlation with RS and RH. When the RS and RH rate were fast, ROC maintained a dynamic and stable state of low soil content. Stand density could regulate RH by affecting soil labile organic carbon, an essential path for stand density to regulate soil respiration. Given soil carbon pool significance in forest ecosystems, Continuous research on soil respiration and stand density is suggested to bridge the gaps in our comprehension of the Regulation of Forest Management on forest soil carbon pool.
Expression Profile of Exosome-associated MicroRNA Derived from Human Red Blood Cell Suspensions During Storage: Predicting microRNA-1246 and microRNA-150-3p Exert Essential Roles in Transfusion-related Immunomodulation
Authors: Kong Yujie , Tian Xue , He Rui , Li Chenyue , Xu Haixia , Tian Li , Liu Zhong 
Published: January 2021

Abstract
Background: Transfusion-related immunomodulation (TRIM) can be caused by exosomes and microRNA (miRNA) is one of the critical functional components in exosomes. This study intends to investigate the differences in the expression of exosomal miRNAs in red blood cell (RBC) suspensions at different storage times, also the potential functions related to TRIM of the abundant miRNAs. Methods: Twenty-five bags of RBC suspensions were selected randomly, and exosomes were separated by ultracentrifugation at different storage times. Isolated exosomes were identified by Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), and Western Blot (WB). Exosomal miRNA profiles were analyzed using genechip in 5 RBC suspension samples, and the miRNA expressions between different storage times were compared. For the statistically upregulated microRNAs, the bioinformation of their predicted target genes was analyzed. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to identify the miRNAs upregulated more than 10 folds at 5weeks storage time in 20 RBC suspension samples.Results: The detection of the gene chip showed most exosomal miRNAs were up-regulated as storage time increases. Compared to RBC suspensions stored for 1 week, that kept for 5 weeks had 539 differential miRNA expressions, among which 159 were significantly different (P<0.05) and 148 (93.08%) were up-regulated. For the bioinformatic analysis, significant immunoregulatory annotations related to thyroid hormone, mitogen-activated protein kinase (MAPK), focal adhesion, and ras signaling pathway were found. The top 17 miRNAs were validated by qRT-PCR, and the results showed miRNA-1246 and miRNA-150-3p were the most significantly enriched miRNAs (more than 150 folds during 5weeks storage).Conclusions: As storage time increased, various exosomal miRNAs in RBC suspensions accumulated and involved multiple immuno-signaling pathways. The predominantly accumulated miRNA-1246 and miRNA-150-3p were confirmed to participate in pro-inflammation responses and immune-regulation, which might exert essential roles in TRIM.
Risk Factors Associated with Disease Severity and Clinical Outcomes for COVID-19 in Wuhan, China
Authors: Liu Yun , Wu Hao , Zhu Bei , Yang Yi , Cheng Peng , Huang Chaolin , Wu Wenjuan , Zhao Weihong , Zhang Jinsong 
Published: January 2021

Abstract
Background: A new type of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appeared in Wuhan, China. However, the risk factors and characteristics related to the severity of the disease and its outcomes need to be further explored.Methods: In this retrospective study, we evaluated COVID-19 patients with severe disease and those who were critically ill, as diagnosed at Jinyintan Hospital (Wuhan, China). The demographic information, clinical characteristics, complications, and laboratory results for the patients were evaluated. Multivariate logistic regression methods were used to analyze risk factors related to hospital deaths.Results: The 235 COVID-19 patients included were divided into a severe group of 183 (78%) and a critical group of 52 (22%). Of these patients, 185 (79%) were discharged, and 50 (21%) died during hospitalization. In multivariate logistic analyses, age (OR=1.07, 95% CI 1.02-1.14, P=0.009), critical disease (OR=48.23, 95% CI 10.91-323.13, P<0.001), low lymphocyte counts (OR=15.48, 95% CI 1.98-176.49, P=0.015), elevated interleukin 6 (IL-6) (OR=9.11, 95% CI 1.69-67.75, P=0.017), and elevated aspartate aminotransferase (AST) (OR=8.46, 95% CI 2.16-42.60, P=0.004) were independent risk factors for adverse outcomes.Conclusions: The results show that advanced age (> 64 years), critical illness, low lymphocyte levels, and elevated IL-6 and AST were factors for the risk of death for COVID-19 patients who had severe disease and those who were critically ill.
Central Administration of Sargahydroquinoic Acid Elevates Peripheral Thermogenic Signaling and Ameliorates High Fat Diet-Induced Obesity
Authors: Kim Doyeon , Kim Hyeung-Rak , Lee Yuna , Hwang Hongik , Rhim Hyewhon , Choi Chun Whan , Lee Bonggi , Kim Min Soo 
Published: January 2021

Abstract
Sargassum serratifolium (C.Agardh) C.Agardh, a marine brown alga, has been consumed as food and traditional medicine in Asia. A previous study showed that the meroterpenoid-rich fraction of an ethanolic extract of Sargassum serratifolium (MES) induced adipose tissue browning and suppressed diet-induced obesity and metabolic syndrome. Sargahydroquinoic acid (SHQA) is a major component in MES. However, it is unclear whether SHQA can regulate energy homeostasis through the central nervous system. To figure it out, SHQA was administrated through the third ventricle of the hypothalamus in high-fat diet-fed C57BL/6 mice and investigated its effects on energy homeostasis. Bath application of SHQA increases the intrinsic neuronal excitability of hypothalamic ARC neurons in acutely prepared brain slices. Thus, we further tested its effect on energy balance. Chronic administration of SHQA into the brain reduced bodyweight without a change in food intake and improved metabolic syndrome-related phenotypes. Cold experiments and biochemical analyses indicate that SHQA elevates thermogenic signaling pathways evidenced by an increase in body temperature and UCP1 signaling in white and brown adipose tissues. As partial mechanisms, SHQA significantly elevated mRNA levels of genes associated with sympathetic outflow and GABA signaling pathways. Our data indicate that hypothalamic injection of SHQA elevates peripheral thermogenic signaling and ameliorates diet-induced obesity.
Sijunzi Tang and gefitinib cooperate to inhibit Lung cancer progression by modulating the pre-metastatic niche
Authors: Zhang Mingyu , Chen Wei , Li Ruisheng , Li Xingjie , Li Chunyu , Li Guohui 
Published: January 2021

Abstract
Background:Although targeted therapies usually trigger great initial responses in patients, the efficacy is transient due to tumor metastasis. The formation of pre-metastatic niche was proposed as the main cause for metastasis, of which the blocking way may be a potential method for inhibiting metastasis. Sijunzi Tang (SJZ), as a complementary drug for targeted therapy, can reduce the recurrence and metastasis of tumors and prolong the survival time of patients. However, how SJZ regulates the formation of pre-metastatic niche to improve the efficacy of targeted therapy remains unclear.Methods: Here, we investigated the anti-tumor activity and immunological mechanism of SJZ plus gefitinib based on pre-metastatic niche in Lewis lung carcinoma (LLC) incubated spontaneous metastatic mouse model, using histopathology and immunological methods.Results:The results showed that SJZ can improve the effect of gefitinib by inhibiting tumor cell growth, promoting tumor cell apoptosis and preventing metastasis in the lung. Besides, SJZ plus gefitinib could inhibit tumor cell aggregation and the expression of characteristic proteins of Mmp2 and Mmp9 in the lung areas of mice. We also confirmed that SJZ could downregulate the expression levels of c-kit and VEGFR2 on DCs, c-kit on neutrophils, c-kit, VEGFR2 on B lymphocyte in the blood, and c-kit and CXCR1 on monocytes in the lung; Gefitinib could decrease the expression levels of c-kit and VEGFR2 on DCs in the blood and c-kit and CXCR1 on monocytes in the lung, but increase the amount of c-kit+ monocytes in the blood. SJZ plus gefitinib decrease the proportion of c-kit+ and CXCR1+ monocytes in the lung. SJZ could regulate pro-metastatic inflammatory responses represented by down-regulating the expression of IL-23，RANTES, GRO-α against that of gefitinib. Moreover, there were significant rises in the expression level of IL-12p70 and IL-15，while declines in the expression level of IL-1β、IL-18、GRO-α in co-treatment group.Conclusions: This work identified the immune cells and cytokines in pre-metastatic niche associated with lung cancer affected by gefitinib and SJZ, and further revealed the immunological mechanism of SJZ improving the efficacy of gefitinib.
Bioremediation Potential of Duckweed (Lemna Gibba) in The Decolorization of C.I. Basic Green 4 and Chlorophyll a Fluorescence Analysis: Understanding Plant Performance
Authors: Singh Hanwant , Raj Shani , Kumar Deepak , Sharma Shubhangani , Bhatt Upma , Soni Vineet 
Published: January 2021

Abstract
In this research, the duckweed (Lemna gibba) potential has been investigated spectrophotometrically as an obvious bioagent for the biological decolorization of organic dye Basic Green 4 (BG4). Experiment result showed that L. gibba has a potent ability to extract BG4 from contaminated water. The study showed that for better results the temperature at 25-30 oC and pH 8.0 is considered to be optimum. A significant induction in SOD, GPOD and CAT activity was observed in L. gibba treated with 15 and 30 mg / l BG4, respectively, after 24 hours of biodecolorization process. It was observed during five repeated batch run that L. gibba showed comparable efficiency in dye decolorization. The chlorophyll fluorescence analysis also shows that photosynthetic apparatus of L. gibba is highly tolerant of BG4. The overall results of the observations here demonstrate the duckweed L. gibba can be used as a potent biodegrading organism for BG4.
Keywords-Driven and Weight-aware Paper Recommendation via Paper Correlation Pattern Mining
Authors: Liu Hanwen , Hou Jun , Li Qianmu , Jiang Jian 
Published: January 2021

Abstract
Currently, readers often prefer to search for their interested papers based on a set of typed query keywords. As the keywords of a paper is often limited, paper recommender systems often need to recommend a set of papers which collectively satisfy the readers’ keyword query. However, the topics of recommended papers are probably not correlated with each other, which fail to meet the readers’ requirements on in-depth and continuous academic research. Furthermore, although existing paper citation graphs can model the papers’ correlations, they often face the data sparse problem which blocks accurate paper recommendations. To address these issues, we propose a keywords-driven and weight-aware paper recommendation approach, named LP-PRk+w (link prediction-paper recommendation), based on a weighted paper correlation graph. Concretely, we firstly optimize the existing paper citation graph modes by introducing a weighted similarity, after which we obtain a weighted paper correlation graph. Then we recommend a set of correlated papers based on the weighted paper correlation graph and the query keywords from readers. At last, we conduct large-scale experiments on a real-world Hep-Th dataset. Experimental results demonstrate that our proposal can improve the paper recommendation performances considerably, compared to other related solutions.
Dialectical Behaviour Therapy as a Transdiagnostic Treatment for Common Psychiatric Disorders: Study Protocol for a Systematic Review
Authors: Tan Michelle Yan Ling , McConnell Bridget , Barlas Joanna 
Published: January 2021

Abstract
BackgroundDialectical Behaviour Therapy (DBT) is one of the most well-known interventions for treating Borderline Personality Disorder, but has been increasingly adapted for use with other psychiatric disorders. Standard DBT consists of four treatment modes, delivered over the course of a full year. Adaptations made to DBT to suit other patient populations include changes to modes of delivery, treatment length, and other components such as skills modules taught to patients. In some cases, interventions from other evidence-based therapies for specific presenting problems or clinical concerns are incorporated into the treatment. This study aims to synthesize the existing evidence on DBT as a treatment for various types of psychiatric conditions.MethodsA systematic review and planned meta-analysis will be conducted according to the procedures outlined in the Cochrane Handbook for Systematic Reviews of Interventions (Higgins et al., 2019). Outcomes will be reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines (Moher et al., 2009). A search strategy was devised to combine domains of various clinical presentations and the treatment approach (i.e., DBT).DiscussionResults from this study will deepen understanding of the effectiveness of DBT in treating various psychiatric conditions, and the clinical populations with which it would be most appropriate for use.Systematic review registrationPROSPERO, CRD42020168550
Low-level Laser Therapy in the Acceleration of Fixed Orthodontic Tooth Movement: Protocol for a Systematic Review and Network Meta-analysis
Authors: Guo Ziyuan , Zhou Lijuan , Feng Chong , Chang Xiao , Han Kaifang , Wang Xuan , Lv Shuxin , Zhang Jiaxi , Zhang Xizhong 
Published: January 2021

Abstract
Background: Orthodontic treatment is a long-term therapy with time-related adverse effects. Low-level laser therapy (LLLT) has been used in the clinic as an intervention to accelerate orthodontic tooth movement (OTM) and prevent adverse effects. Previous systematic reviews have evaluated the effectiveness of LLLT in the acceleration of OTM. However, it remains unclear which treatment parameters are appropriate for LLLT. This review will evaluate the efficacy of LLLT on the acceleration of fixed OTM and the prevention of adverse effects.Methods: Several electronic databases will be searched. Grey literature also will be retrieved. Parallel or clustered randomised controlled trials that evaluated the effectiveness of different LLLTs on the acceleration of fixed OTM will be included. Two researchers will independently screen all titles, abstracts, and full-text articles retrieval, as well as data extraction. The risk bias of each study will be appraised using the Cochrane Risk of Bias tool (RoB 2.0). The primary outcome will be the efficacy of LLLT on the acceleration of fixed OTM (e.g., canine movement distance, orthodontic treatment duration). Secondary outcomes will be the effectiveness of LLLT to reduce orthodontic adverse effects such as pain and root resorption. The mean difference (MD) and relative risk (RR) with corresponding 95% confidence intervals (CI) will be chosen as effective measures for continuous and binary outcomes, respectively. When feasible, both fixed and random-effects pairwise meta-analyses and frequentist network meta-analyses will be conducted.Discussion: This network meta-analysis will compare the efficacy of LLLT on the acceleration of fixed OTM as well as whether LLLT can prevent adverse effects of this orthodontic treatment. By integrating the current evidence from direct and indirect comparisons and ranking all included interventions, our findings have the potential to help clinicians achieve more effective treatment goals.Systematic review registration: PROSPERO CRD42020175850
A New Scoring System for Predicting Short-term Outcomes in Critically-ill Acute Decompensated Heart Failure
Authors: Mo Ran , Yu Li-tian , Tan Hui-qiong , Wang Yang , Yang Yan-min , Liang Yan , Zhu Jun 
Published: January 2021

Abstract
Background: Acute decompensated heart failure (ADHF) contributes millions of emergency department (ED) visits and it is associated with high in-hospital mortality. The aim of this study was to develop and validate a multiparametric score for critically-ill ADHF patients.Methods: In this single-center, retrospective study, a total of 1268 ADHF patients were enrolled and divided into derivation (n=1014) and validation (n=254) cohorts. The primary endpoint was any in-hospital death, cardiac arrest or utilization of mechanical support devices. Logistic regression model was preformed to identify risk factors and build the new scoring system. The assigning point of each parameter was determined according to its β coefficient. The discrimination was validated internally using C statistic and calibration was evaluated by the Hosmer-Lemeshow goodness-of-fit test. Results: We constructed a predictive score based on six significant risk factors [systolic blood pressure (SBP), white blood cell (WBC) count, hematocrit (HCT), total bilirubin (TBIL), estimated glomerular filtration rate (eGFR) and NT-proBNP]. This new model was computed as (1×SBP<90mmHg)+(2×WBC>9.2×109/L)+(1×HCT≤0.407)+(2×TBIL>34.2μmol/L)+(2×eGFR<15ml/min/1.73m2)+(1×NTproBNP≥10728.9ng/ml). The C statistic for the new score was 0.758 (95%CI 0.667 to 0.838) higher than APACHE Ⅱ, AHEAD and ADHERE score. It also demonstrated good calibration for detecting high-risk patients in the validation cohort (χ2 =6.681, p=0.463).Conclusions: The new score including SBP, WBC, HCT, TBIL, eGFR and NT-proBNP might be used to predict short-term prognosis of critically-ill ADHF patients.