bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel non-metabolic function of lactate in remodeling uterine receptivity by inducing endometrial histone lactylation, revealed by a proteomic atlas of maternal-fetal crosstalk in sheep
Authors: Yang Qianying , Liu Juan , Wang Yue , Zhao Wei , Wang Wenjing , Cui Jian , Yang Jiajun , Yue Yuan , Chu Meiqiang , Lv Qingji , Ma Lizhu , Tang Yawen , Hu Yupei , Miao Kai , Zhao Haichao , Tian Jianhui , An Lei 
Published: May 2021

Abstract
Well-orchestrated maternal-fetal crosstalk involves secreted ligands, interacting receptors, and coupled pathways between the conceptus and endometrium. However, previous researches mainly focused on either the conceptus or endometrium in isolation. The lack of integrated analysis, especially on protein levels, has made it challenging to advance our understanding of the crosstalk. Herein, focusing on ligand–receptor complexes and coupled pathways at maternal-fetal interface in sheep, a well-established embryo implantation model, we provide the first comprehensive proteomic atlas of ligand-receptor-pathway cascades that are essential for implantation. Based on these candidate interactions, we further revealed the physical interaction of albumin-claudin 4 and their role in facilitating embryo attachment to endometrium. More interestingly, we demonstrated a novel non-metabolic function of enhanced conceptus glycolysis in remodeling uterine receptivity, by inducing endometrial histone lactylation, a newly identified histone modification. Our results from in vitro and in vivo models supported the essential role of lactate, as a key pregnancy recognition signal, in regulating redox homeostasis and apoptotic balance to ensure successful implantation. Our study identified many putative molecular and cellular mechanisms that fine-tuned conceptus-endometrium crosstalk during implantation, thus providing important clues for developing potential clinical intervention strategies to improve pregnancy outcomes following both natural conception and assisted reproduction.
Gender differences in mathematical achievement development: A family psychobiosocial model
Authors: Chiu Mei-Shiu , Chen Bo-Jen 
Published: May 2021

Abstract
This study proposes a family psychobiosocial model on gender differences in cognitive development. Specifically, the aim is to investigate how family biological, socioeconomic, and psychological factors predict child mathematics achievement (MAch) development. The data was obtained from the Millennium Cohort Study. Children’s pattern construction scores collected at ages 5 and 7 years worked as MAch (n = 18,497). The predictors were family data collected when the children were 9 months. The results of path analyses for all students indicate that all three factors in the family psychobiosocial model play some roles in children’s MAch development. Analyses for the female and male students separately reveal that girls’ positive MAch development was significantly predicted by four psychobiosocial factors (fewer mother in-pregnancy alcohol intakes, more family income, higher mother education levels, and more mother cognitive stimulation); boys’ MAch development is predicted by only one factor (higher mother education levels). The results support the psychobiosocial model as a whole. Family psychobiosocial factors, especially social factors, impact children’s cognitive development more for females than for males.
Longitudinal immune profiling of a SARS-CoV-2 reinfection in a solid organ transplant recipient
Authors: Klein Jon , Brito Anderson , Trubin Paul , Lu Peiwen , Wong Patrick , Alpert Tara , Pena-Hernandez Mario , Haynes Winston , Kamath Kathy , Liu Feimei , Vogels Chantal , Fauver Joseph , Lucas Carolina , Oh Ji Eun , Mao Tianyang , Silva Julio , Wyllie Anne , Muenker M. Catherine , Casanovas-Massana Arnau , Moore Adam , Petrone Mary , Kalinich Chaney , TEAM YALE IMPACT RESEARCH , Cruz Charles Dela , Farhadian Shelli , Ring Aaron , Shon John , Ko Albert , Grubaugh Nathan , Goldman-Israelow Benjamin , Iwasaki Akiko , Azar Marwan 
Published: May 2021

Abstract
The underlying immunologic deficiencies enabling SARS-CoV-2 reinfections are currently unknown. Here we describe a renal-transplant recipient who developed recurrent, symptomatic SARS-CoV-2 infection 7 months after primary infection. To elucidate the immunological mechanisms responsible for reinfection, we performed longitudinal profiling of cellular and humoral responses during both primary and recurrent SARS-CoV-2 infection. We found that the patient responded to the primary infection with transient, poor-quality adaptive immune responses that was further compromised by intervening treatment for acute rejection of the renal allograft prior to reinfection. Importantly, we identified the development of neutralizing antibodies and humoral memory responses prior to SARS-CoV-2 reinfection. However, these neutralizing antibodies failed to confer protection against reinfection, suggesting that additional factors are required for efficient prevention of SARS-CoV-2 reinfection. Further, we found no evidence supporting viral evasion of primary adaptive immune responses, suggesting that susceptibility to reinfection may be determined by host factors rather than pathogen adaptation.
Total excision of lumps and reconstructed the scrotum wall in situ for multiple epidermal cysts of scrotum: a case report
Authors: Wu ShangChao , Li GuanYi , Xu Hui , Niu Wenbin , Zhong Xin , Li Fang 
Published: May 2021

Abstract
BackgroundEpidermoid cysts are the common benign epithelial cysts that may occur in the dermis or subcutaneous tissue. It have been observed in several organs,especially in the skin of the scalp, ear, face, and back.However, the multiple epidermal cysts over the scrotum is a rare condition. We report a case of total excision of lumps and reconstructed the scrotum wall in situ for multiple epidermal cysts of scrotum.Case presentation:A 23-year-old man presented with multiple lumps on the scrotum skin for more than 7 years. On physical examination, multiple lumps were found arising from the scrotal skin, the largest one measuring 1.5 cm. Ultrasonography of the local part indicated testes of normal consistency without free fluid in the tunica of scrotum. The laboratory workup revealed that the blood cell, blood electrolyte and thyroid hormone were within the normal limits.A total excision of scrotal lumps and reconstructed the scrotum wall in situ was performed in this case. And the histopathologic of the specimen is demonstrated an epidermal cysts.ConclusionMultiple epidermal cysts over the scrotum are extremely rare, and correct diagnosis depends on the clinical history, radiological dates,clinical appearance,physical features and histopathologic analysis. The accumulation of further cases is needed to help understand the precisely histogenesis, diagnosis, and treatment of this disease.
Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes
Authors: Gopinath Ponnusamy Manogaran , Twayana Krishna Sundar , Ravanan Palaniyandi , Thomas John , Mukherjee Amitava , Chandrasekaran Natarajan 
Published: May 2021

Abstract
Background
Today, cosmetic usage has become customary in both men and women towards improving their appearance and increase societal visibility. In this study, the nano-sized (30 to 300 nm) plastic particles were isolated from the commercial face-scrubs and treated on the human keratinocytes.
Results
The adherence studies of polyethylene nano-plastics (PENPs), polystyrene NPs (PSNPs), and face-scrubs isolated nano-plastics (NPs) on the keratin layer revealed a rapid attachment of NPs on the skin from the cosmetics that have short exposure time. This attachment property could facilitate further adherence of protein molecules on NPs and the protein-corona formation. The protein-corona mimics protein aggregates, thereby triggers the macropinocytosis followed by the macropinolysosomal process in the cell. Then the internalized NPs induced the concentration-dependent cytotoxic, cytostatic and cytoprotective activity in keratinocytes. Both single dose and chronic long-term exposure of lethal and sub-lethal concentrations of NPs resulted in the oxidative stress-mediated down-regulation of cell growth and proliferation inhibition. Autophagy and premature aging were also observed in the NPs internalized HaCaT cells incubated in a fresh, NPs-free medium.
Conclusion
At the outset, this work provides insight into the NPs concentration-dependent regulatory, cytoprotective, and cytotoxic effects in HaCaT cells. Further studies are required to identify the detailed mechanisms of NPs toxicity and cytoprotective events in cells at the molecular level.
Association Between Maternal Exposure to PM10 and Risk of Anorectal Atresia/Stenosis in Offspring: A Population-Based Case-Control Study in Liaoning Province, China
Authors: Zhang Tie-Ning , Wu Qi-Jun , Huang Yan-Hong , Li Jing , Chen Zong-Jiao , Li Li-Li , Chen Yan-Ling , Liu Shu , Jiang Cheng-Zhi , Zhao Yu-Hong 
Published: May 2021

Abstract
Background: The potential association between maternal exposure to PM10 ranging from 3 months prior to conception to the end of the early trimester and the risk of anorectal atresia/stenosis in offspring have not been established. Thus, we determined the association in this study.Methods: We recruited 713 patients with anorectal atresia/stenosis and 7950 randomly selected healthy offspring from the Maternal and Child Health Certificate Registry of Liaoning Province and delivered between 1 January 2010 and 31 December 2015. Monthly PM10 concentrations were retrieved from the Environment Protection Bureau of each city in Liaoning Province. We established a multivariable logistic regression model to calculate the adjusted odds ratios (ORs) and 95% confidence intervals (CIs).Results: Maternal exposure to PM10 was associated with an increased risk for anorectal atresia/stenosis in offspring during the three months prior to conception (per 10 μg/m3 increment: OR = 1.15, 95% CI = 1.03–1.20; per SD [27 μg/m3] increment: OR = 1.33, 95% CI = 1.09–1.63) and the first trimester (per 10 μg/m3 increment: OR = 1.08, 95% CI = 1.00–1.17; per SD [28 μg/m3] increment: OR = 1.26, 95% CI = 1.01–1.57). Evaluation of the association with a shorter exposure window (1 month) revealed a positive association between anorectal atresia/stenosis and PM10 from the 3rd month prior to pregnancy to each month of the 1st trimester.Conclusion: Maternal exposure to PM10 three months prior to conception and during the 1st trimester was associated with and increased risk of anorectal atresia/stenosis in the offspring. Future perspective studies are needed to confirm our findings.
WITHDRAWN: Changes In Monthly Streamflow In The Hindukush-Karakoram-Himalaya Region of Pakistan Using Innovative Polygon Trend Analysis
Authors: Ahmed Naveed , Wang Genxu , Booij Martijn J , Ceribasi Gokmen , Bhat Mohd Sultan , Ceyhunlu Ahmet Iyad , Ahmed Adeel 
Published: May 2021

Abstract
The present study explored the changes in monthly streamflow in the Hindukush-Karakoram-Himalaya (HKH) region within Pakistan using the recently developed trend analysis method known as Innovative Polygon Trend Analysis (IPTA). The monthly streamflow data of 34 gauging stations installed in the HKH region was analyzed, and the Pettitt test was applied to check the homogeneity of the time series. The entire study area was divided into 13 sub-basins, and then changes in monthly streamflow of each sub-basin were explored using the IPTA method. The streamflow of high elevated glaciated and snow/ice-covered sub-basins (e.g., Shyok, Astore, and Chitral) have increased in high flow months (June-August), where there is a downward trend for the Hunza sub-basin in these months. In the Gilgit sub-basin, a transition occurred from no trend in May to a decreasing trend from June to August. The upstream gauges of Swat and Kabul sub-basins showed an increasing trend throughout the year, while downstream gauging stations in the same sub-basins exhibited a strong decreasing trend only in high flow months (June-August). The upper reaches of the Indus part in the Upper Indus Basin (UIB) showed increasing trends in high flow months (June-August), however the downstream gauges of UIB showed decreasing trends throughout the year. Overall, only the glaciated and snow/ice-covered sub-basins experienced increasing trends, while most other sub-basins faced decreasing trends in high flow months and increasing trends in low flow months (October - March). The enhancement of the seasonal pattern of streamflow in the upper reaches of UIB is possibly due to the melting of snow and ice that potentially influence floods and hydropower generation. The results of this study can result in a better understanding of the hydrology of the HKH region and support sustainable water management.
Heavy Atom Oriented Orbital Angular Momentum Manipulation in Metal-Free Organic Phosphors
Authors: Shao Wenhao , Jiang Hanjie , Zimmerman Paul , Kim Jinsang 
Published: May 2021

Abstract
Metal-free purely organic phosphors (POPs) are emerging materials for display technologies, solid-state lighting, and chemical sensors. The past decade has seen the promising utility of the El-Sayed rule and heavy atom effects in the design of POPs, and efficient matrix engineering to boost emission efficiencies. However, due to limited contemporary design strategies, the intrinsic spin-orbit coupling (SOC) efficiency of POPs remains low and their emission lifetime is pinned in the millisecond-second regime. Here, we report a universally applicable methodology to synergistically manipulate the main descriptors in SOC - heavy atom effect and orbital angular momentum, assisted by a novel set of natural-transition-orbital-based computation methods to visualize angular momentum descriptors in molecular design. Prototype POPs with efficient room-temperature phosphorescence were designed with SOC efficiencies boosted beyond 102 cm‑1 and lifetime pushed below the millisecond regime. Experimental verification for our novel design rule was conducted through systematic computation-assisted design achieving discrete tuning of heavy atom effects and orbital angular momentum.
Effect of Melatonin on Clinical Outcome of Patients with Repeated Cycles after Failed Cycles of In Vitro Fertilization and Intracytoplasmic Sperm Injection
Authors: Zhu Qi , Wang Kaijuan , Zhang Chao , Chen Beili , Zou Huijuan , Zou Weiwei , Xue Rufeng , Ji Dongmei , Yu Zhaojuan , Rao Bihua , Cao Yunxia , Ding Ding , Zhang Zhiguo 
Published: May 2021

Abstract
Background: Melatonin (MT), as a powerful antioxidant, can effectively ameliorate the in vitro development of animal embryos, but few studies have been made on human embryos. Therefore, we investigated whether the application of MT in embryo cultures can improve embryonic development and clinical outcomes in patients with repeated cycles after failed in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.Methods: Human immature oocytes from controlled ovarian hyperstimulation cycles were collected for in vitro maturation culture and ICSI fertilization. The obtained embryos were cultured in vitro in medium containing 0, 10-11, 10-9, 10-7 or 10-5 M MT, and 10-9 M was determined to be the optimal concentration. Subsequently, 140 patients who experienced failed IVF/ICSI cycles received 140 cycles of embryo culture in vitro with medium containing 10-9 M MT. High-quality blastocysts were collected and cryopreserved for three months before vitrified-warmed embryo transfer. These culture cycles served as the experimental (10-9 M) group. The control (0 M) group comprised previous failed cycles. The patients were further divided into subgroups of 1, 2 or ≥3 failed cycles. The fertilization and embryo development status were compared.Results: The fertilization, cleavage, high-quality embryo, blastocyst, and high-quality blastocyst rates of the 10-9 M group were significantly higher than 0 M (87.7% vs. 83.6%, p <0.01; 94.1% vs. 90.5%, p <0.01; 58.3% vs. 43.8%, 51.1% vs. 41.8%, 43.4% vs. 22.9%, all p <0.0001). A total of 50 vitrified-warmed cycle transfers were performed. A clinical pregnancy rate of 40% was obtained. Conclusion: The application of MT to embryo cultures in vitro improved embryonic development in patients with repeated cycles after failed IVF/ICSI cycles and obtained good clinical outcomes. The optimal concentration of MT was 10-9 M.Trial registration: Name of the registry: Effect of melatonin on clinical outcome of patients with repeated cycles after failed cycles of in vitro fertilization and intracytoplasmic sperm injection; registration number: ChiCTR2100045552; date of registration: April 19, 2021(Retrospectively registered); URL of trial registry record: www.medresman.org.cn.
Role of Ultrasound In The Diagnosis of Primary And Recurrent Dermatofibrosarcoma Protuberans
Authors: Zou Min-Hong , Huang Qing , Yang Ting , Jiang Ye , Zhang Luan-jing , Xie Yang , Zheng Rong-Qin 
Published: May 2021

Abstract
Background: Dermatofibrosarcoma protuberans (DFSP) is a rare, low- to intermediate-grade sarcoma, which represents a diagnostic imaging challenge. This study aimed to analyze the clinical and ultrasound features of primary and recurrent DFSP to improve the diagnosis.Methods: Clinical, imaging, and pathological data from a total of 58 patients (23 patients with primary DFSP and 35 patients with recurrent DFSP) were retrospectively reviewed.Results: There was no statistically significant difference in age, sex, tumor size, or echogenicity between the two groups. Most of the primary DFSP lesions involved the overlying dermis and hypodermis, while most of the recurrent DFSP lesions were fixated to more deeply seated structures at the original surgical incision. Red nodules on the skin were found more frequently in the primary group. There were statistically significant differences in the type of lesion and ultrasound tumor morphology (p < 0.050). The lesions in the primary group showed more tentacle-like projections or a “claw” sign, while the lesions in the recurrent group were more commonly oval, lobulated, and irregularly shaped. Hypervascularity was common in both groups.Conclusions: For primary DFSP, a slow-growing, red nodule on the skin involving the overlying dermis and hypodermis, more frequently a hypoechoic mass with tentacle-like projections or a “claw” sign, was observed. For recurrent DFSP, palpable subcutaneous nodules or subcutaneous masses at the original surgical incision and oval, lobulated, and irregularly shaped lesions were more commonly observed. This may be useful for improving diagnostic accuracy.
Lovastatin Production from Cunninghamella blakesleeana under Solid State Fermentation
Authors: Balraj Janani , Murugesan Thandeeswaran , Kalieswaran Vidhya , Jairaman Karunyadevi , Esakkimuthu Devippriya , J Angayarkanni 
Published: May 2021

Abstract
Our earlier paper had established the fact that new soil fungi known as Cunninghamella blakesleeana is potent enough to produce lovastatin significantly. At present, there are no reports on the media optimization for the lovastatin production. Hence, the objective is to optimize the fermentation conditions for lovastatin production by Cunninghamella blakesleeana under Solid State fermentation (SSF) condition through screening the critical factors by one factor at a time and then, optimize the factors selected from screening using statistical approaches. SSF was carried using the pure culture of Cunninghamella blakesleeana KP780148.1 with wheat bran as substrate. Initial screening was performed for physical parameters, carbon sources and nitrogen sources and then optimized the selected parameters through PBD and BBD. Screening result indicated the optimum values of the analysed parameter for the maximal production of lovastatin by Cunninghamella blakesleeana were selected. Out of the nine factors MgSO4, (NH4)2SO4, pH and Incubation period were found to influence the lovastatin production significantly after PBD. The optimal levels of these variables and the effect of their mutual interactions on lovastatin production were determined using BBD surface design. The optimum medium composition was found to be MgSO4(0.2 g/L), (NH4)2 SO4 (12.5 g/L), pH (6) and Incubation period (7 days). Experimental studies showed a yield of 7.39 mg/g at the above optimized conditions which were observed to be very nearby to the predicted value and hence the model was successfully validated. Hence, this is the first report on the optimization of critical parameters for lovastatin production by Cunninghamella blakesleeana.
Efficacy of Interferon β-1a in Treatment of Hospitalized COVID-19 Patients; SBMU Taskforce on the COVIFERON Study
Authors: Fallahzadeh Mohammad , Pourhoseingholi Mohamad Amin , Besharati Sajad , Boroujeni Masoud Ghanbari , Mardani Masoud , Shabani Minoosh , Shokouhi Shervin , Amirdosara Mahdi , Hajiesmaeili Mohammadreza , Gachkar Latif , Roshan Baran , Zangi Masoud , Mirmomeni Golshan , Irvani Seyed Sina Naghibi , Darazam Ilad Alavi 
Published: May 2021

Abstract
Background: Interferons are essential part of the innate immune and have antiviral and immunomodulatory functions. In the lack of definite medication for COVID-19, Interferons can be effective in treatment of severe cases of COVID-19 and may decrease mortality of this disease.Material and methods: This retrospective study was conducted on hospitalized COVID-19 patients in Loghman-Hakim hospital from 20 February 2020 to 20 April 2020, Tehran, Iran. Patients were selected from two groups, first group received Interferon β-1a in addition to standard treatment regimen and patients of second group received standard care. The clinical progression of two groups during their hospital admission have been compared.Results: We studied total number of 395 hospitalized COVID-19 patients. Out of this number, 111 patients (33.5%) died (31.3% of Interferon β-1a (Recigen) group and 34.1% of control group). Mortality rate indicated no statistically significant difference between groups, however for patients who hospitalized more than a week, the rate of mortality was lower in Recigen group. The median time of staying in hospital was statistically shorter for patients treated by IFN-β1a. The Odds of death in control groups who hospitalized for at least one week, compared to Recigen group was 3.96 (95% CI: 1.37-11.43, P.value=0.011) Conclusion: This study showed IFN-β1a have significant efficacy in treating severe COVID-19 patients who hospitalized for at least one week and improve the outcomes of patients and decrease the duration of hospitalization.