bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization and flocculation evaluation of a new organic–inorganic hybrid polymer flocculant (PAC‐AM‐DMC)
Authors: Chen Meijun , Zou Changjun , Tang Wenyue , Huang Yushuang , Sun Huahong 
Published: July 2021

AbstractA novel organic–inorganic hybrid flocculant PAC‐AM‐DMC is synthesized using potassium persulfate as an initiator, which was used in industrial wastewater treatment. Comparing with the traditional inorganic flocculant, this new flocculant has the advantage of low dosage and high efficiency. Simultaneously, it is less toxic and more environmentally friendly than the organic flocculant. The organic–inorganic hybrid flocculant PAC‐AM‐DMC demonstrates better flocculation than the commercial single type flocculant (PAC, PAM), and the effect is better than simply combining the two flocculants. It has potential for commercialization. When PAC:(AM + DMC) = 2:8, the initiator is 0.2 wt%, and the flocculant obtained by reacting at 50°C for 4 h has the best effect on sewage treatment. When the pH is 7, the treatment effect of PAC‐AM‐DMC is best with the dosage of 10 ppm. Correspondingly, the amount of flocculant is 10 mg/L, and the turbidity of simulated sewage is 172 nephelometric turbidity unit (NTU), which is over 98% of turbidity removal efficiency. In addition, the prepared hybrid flocculant is nearly non‐corrosive and posed little damage to the equipment. And the hybrid flocculant has excellent salt resistance.
Glycerol‐regulated tough and electroresponsive alginate hydrogels for a muscle‐like biobased polymer actuator with highly sensitive and durable output force
Authors: Yang Junjie , Wang Zhijie , Wang Siyong 
Published: July 2021

AbstractIn this work, with glycerol as a moisturizer, effect and enhancement mechanism of glycerol doping content on the responsiveness, force output and tremor behavior of the muscle‐like biobased polymer actuator (MBPA) were intensively investigated. Then its electroresponsive properties were characterized by a set of valid test methods proposed in detail. Experiments revealed that the optimal glycerol doping content was 3 ml, then MBPA had the best force output and responsiveness characteristics of the maximum about 5.78 mN and 0.230 mN/s, respectively, which were 3.5 and 5.5 times larger than that of the undoped MBPA. Besides, at 5 ml glycerol doping content, tremor amplitude and frequency of MBPA on the force output were around 0.7 mN and three, which were correspondingly 0.27 and 0.12 times lower than the maximum. Moreover, enhancement principle of the MBPA was that doping glycerol could make its surface form a thin membrane to retain moisture inside the MBPA with a stable ion migration environment. Because of small molecules, glycerol was able to interpenetrate with sodium alginate, which destroyed the interaction force but promoted the relative sliding capability between its macromolecular chains, with the number of hydrogen bonds and the rotational activation energy reducing.
Further developing the Frith–Happé animations: A quicker, more objective, and web‐based test of theory of mind for autistic and neurotypical adults
Authors: Livingston Lucy A. , Shah Punit , White Sarah J. , Happé Francesca 
Published: July 2021

AbstractThe Frith–Happé Animations Test, depicting interactions between triangles, is widely used to measure theory of mind (ToM) ability in autism spectrum disorder (ASD). This test began with recording, transcribing, and subjectively scoring participants' verbal descriptions, which consistently found ToM‐specific difficulties in ASD. More recently in 2011, White et al. created a more objective version of this ToM test using multiple‐choice questions. However, there has been surprisingly little uptake of this test, hence it is currently unclear if White et al.'s findings replicate. Further, the lack of an online version of the test may be hampering its use in large‐scale studies and outside of research settings. Addressing these issues, we report the development of a web‐based version of the Frith–Happé Animations Test for autistic and neurotypical adults. An online version of the test was developed in a large general population sample (study 1; N = 285) and online data were compared with those collected in a lab‐based setting (study 2; N = 339). The new online test was then administered to adults with a clinical diagnosis of ASD and matched neurotypical controls (study 3; N = 231). Results demonstrated that the test could successfully be administered online to autistic adults, who showed ToM difficulties compared to neurotypical adults, replicating White et al.'s findings. Overall, we have developed a quicker, more objective, and web‐based version of the Frith–Happé Animations Test that will be useful for social cognition research within and beyond the field of autism, with potential utility for clinical settings.Lay SummaryMany autistic people find it hard to understand what other people are thinking. There are many tests for this ‘mentalising’ ability, but they often take a long time to complete and cannot be used outside of research settings. In 2011, scientists used short silent animations of moving shapes to create a fast way to measure mentalising ability. We developed this into an online test to use in research and clinics to measure mentalising ability in autism.
Determination of human COVID‐19 total antibodies in serum using a time‐resolved fluorescence immunoassay
Authors: Xu Yunjian , Chen Cuicui , Lai Hongrui , He Ying , Guo Guiling , Feng Jianming , Li Laiqing 
Published: July 2021

AbstractCoronavirus disease 2019 (COVID‐19) caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is spreading rapidly around the world. Antibody detection plays an important role in the diagnosis of COVID‐19. Here, we established a new time‐resolved fluorescence immunoassay (TRFIA) to determine COVID‐19 total antibodies. A double‐antigen sandwich TRFIA was optimized and established: recombinant nucleocapsid phosphoprotein (N protein) and spike protein (S protein) of COVID‐19 immobilized on 96‐well plates captured human COVID‐19 antibodies and then banded together with the N/S proteins labeled with europium(III) (Eu3+) chelates, and finally, time‐resolved fluorometry was used to measure the fluorescence values. We successfully established a TRFIA method for the detection of human COVID‐19 total antibodies, and the cutoff value was 2.02. There was no cross‐reactivity with the negative reference of the National Reference Panel for IgM and IgG antibodies to COVID‐19. The CV of the precision assay was 3.19%, and the assay could be stored stably for 15 days at 37°C. Compared with that of the colloidal gold method and chemiluminescence method, the sensitivity of the TRFIA method was higher, and the false positive/negative rate was lower. This established TRFIA has high sensitivity, accuracy, and specificity, which indicates that this method provides a new detection method for the high‐throughput routine diagnosis of COVID‐19.
Gamma‐synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma
Authors: Takemura Yusuke , Ojima Hidenori , Oshima Go , Shinoda Masahiro , Hasegawa Yasushi , Kitago Minoru , Yagi Hiroshi , Abe Yuta , Hori Shutaro , Fujii‐Nishimura Yoko , Kubota Naoto , Masuda Yuki , Hibi Taizo , Sakamoto Michiie , Kitagawa Yuko 
Published: July 2021

AbstractGamma‐synuclein (SNCG) promotes invasive behavior and is reportedly a prognostic factor in a range of cancers. However, its role in biliary tract carcinoma (BTC) remains unknown. Consequently, we investigated the clinicopathological significance and function of SNCG in BTC. Using resected BTC specimens from 147 patients with adenocarcinoma (extrahepatic cholangiocarcinoma [ECC, n = 96]; intrahepatic cholangiocarcinoma [ICC, n = 51]), we immunohistochemically evaluated SNCG expression and investigated its correlation with clinicopathological factors and outcomes. Furthermore, cell lines with high SNCG expression were selected from 16 BTC cell lines and these underwent cell proliferation and migration assays by siRNAs. In the results, SNCG expression was present in 22 of 96 (22.9%) ECC patients and in 10 of 51 (19.6%) ICC patients. SNCG expression was significantly correlated with poorly differentiated tumor in both ECC and ICC (p = 0.01 and 0.03, respectively) and with perineural invasion and lymph node metastases in ECC (p = 0.04 and 0.003, respectively). Multivariate analyses revealed that SNCG expression was an independent poor prognostic factor in both OS and RFS in both ECC and ICC. In vitro analyses showed high SNCG expression in three BTC cell lines (NCC‐BD1, NCC‐BD3, and NCC‐CC6‐1). Functional analysis revealed that SNCG silencing could suppress cell migration in NCC‐BD1 and NCC‐CC6‐1 and downregulate cell proliferation in NCC‐CC6‐1 significantly. In conclusion, SNCG may promote tumor cell activity and is potentially a novel prognostic marker in BTC.
Clinical predictors and impact of postoperative mean gradient on outcome after transcatheter edge‐to‐edge mitral valve repair
Authors: Oguz Didem , Padang Ratnasari , Pislaru Sorin V. , Nkomo Vuyisile T. , Mankad Sunil V. , Alkhouli Mohamad , Guerrero Mayra , Reeder Guy S. , Eleid Mackram F. , Rihal Charanjit S. , Thaden Jeremy J. 
Published: July 2021

AbstractBackgroundThe predictors and clinical significance of increased Doppler‐derived mean diastolic gradient (MG) following transcatheter edge‐to‐edge mitral valve repair (MVTEER) remain controversial.ObjectiveWe sought to examine baseline correlates of Doppler‐derived increased MG post‐MVTEER and its impact on intermediate‐term outcomes.MethodsPatients undergoing MVTEER were analyzed retrospectively. Post‐MVTEER increased MG was defined as >5 mmHg or aborted clip implantation due to increased MG intraprocedurally. Baseline MG and 3D‐guided mitral valve area (MVA) by planimetry were retrospectively available in 233 and 109 patients.Results243 patients were included; 62 (26%) had MG > 5 mmHg post‐MVTEER or aborted clip insertion, including 7 (11%) that had aborted clip implantation. Mortality occurred in 63 (26%) during a median follow up of 516 days (IQR 211, 1021). Increased post‐MVTEER MG occurred more frequently in females (44% vs. 16%, p <  0.001), those with baseline MVA <4.0 cm2 (71% vs. 16%), baseline MG ≥4 mmHg (61% vs. 20%), or multiple clips implanted (33% vs. 21%, p = 0.04). Increased post‐MVTEER MG was associated with increased subsequent mortality compared to those with normal gradient (HR 1.91 95% CI 1.15–3.18 p = 0.016) as was aborted clip insertion compared to all others (HR 5.23 95% CI 2.06–13.28 p <  0.001).ConclusionsSmaller baseline MVA and increased baseline MG are associated with increased MG post‐MVTEER and patients with a Doppler‐derived post‐MVTEER MG >5 mmHg suffered excess subsequent mortality. In high risk patients considered for MVTEER, identification of those at risk of iatrogenic mitral stenosis with MVTEER is important as they may be optimally treated with alternate surgical or transcatheter therapies.
Rosai‐Dorfman disease: A case report of asymptomatic isolated renal involvement
Authors: Abrishami Alireza , Ziaeefar Pardis , Ebrahimi Sara , Khalili Nastaran , Nouralizadeh Akbar , Farjad Reza 
Published: July 2021

AbstractA possible diagnosis of RDD should be kept in mind when encountering a patient with raised plasma creatinine levels and renal mass. Timely diagnosis and management of RDD will help prevent future kidney loss.
The chronological change in transvaginal ultrasound images of a hemorrhagic ovarian cyst observed during infertility treatment: A case report and literature review
Authors: Fujii Yu , Wakimoto Yu , Omote Maya , Sugiyama Yukiko , Ukita Yuji , Kato Toru , Fukui Atushi , Shibahara Hiroaki 
Published: July 2021

Key Clinical MessageInsights gained from chronological ultrasonogram images in the current case report will provide useful information for diagnosing and conservatively treating HOC. This could help avoid unnecessary laparotomy.
The first reported case of Noonan syndrome complicated with hepatocellular carcinoma
Authors: Kakizaki Satoru , Uehara Daisuke , Tojima Hiroki , Suga Takayoshi , Yamazaki Yuichi , Sato Ken , Kubo Norio , Shirabe Ken , Yokota Takayuki , Shibuya Kei , Maehara Tatsurou , Yokoo Hideaki , Naganuma Atsushi , Uraoka Toshio 
Published: July 2021

AbstractNoonan syndrome is a genetic multisystem disorder and is associated with mutation of genes encoding the proteins in the RAS‐MAPK pathway. We reported the first case of Noonan syndrome complicated with hepatocellular carcinoma.
Granulomatosis with polyangiitis (Wegener’s) complicated by splenic rupture and severe acute respiratory distress syndrome: A case report
Authors: Ahmad Yannis , Morawietz Gabrielle , Ksouri Hatem , Schefold Joerg C. , Zuercher Patrick 
Published: July 2021

AbstractEven in the absence of disease‐specific radiological signs of granulomatosis with polyangiitis (GPA), severe intrapulmonary GPA may be present. Rapidly establishing the diagnosis with a confirmatory biopsy is key to initiate lifesaving therapy.
Acute epiglottitis after COVID‐19 infection
Authors: Renner Andreas , Lamminmäki Satu , Ilmarinen Taru , Khawaja Tamim , Paajanen Juuso 
Published: July 2021

AbstractIn patients with acute epiglottitis, the possibility of COVID‐19 should be ruled out. Repeated nasofiberoscopy examinations or a tracheostomy, which may produce infectious aerosols, may be required.
A rare cause of cyanosis: Congenital methemoglobinemia
Authors: Guedri Rahma , Missaoui Nada , Essaddam Leila , Ben Becher Saayda 
Published: July 2021

AbstractCongenital Methemoglobinemia is a rare condition that may mimic congenital heart diseases. There are two types of congenital Methemoglobinemia. The type I is usually benign. The enzyme deficiency is limited to red blood cells. Clinically, the patient presents cyanosis without neurological disorders. Whereas, in type II, cyanosis is associated with severe neurological impairment.
Subcutaneous injection of organophosphate (Fenitrothion)—Management of preventing the appearance of toxic symptoms: A case report
Authors: Nakamura Hitomi , Hirayama Ichiro , Hiruma Takahiro , Doi Kent 
Published: July 2021

AbstractThere is a risk of unnecessary extensive incision because of swelling after the subcutaneous injection; however, removing completely the injected organophosphate by making a skin incision before the appearance of toxic symptoms could reduce sequelae.
A rare initial presentation of a thymic neuroendocrine tumor as Cushing's syndrome
Authors: Matarneh Ahmad S. , Hamad Abdelrahman O. , Hamad Mohammad K. , Elouzi Elhadi B. , Mahmood Nabil S. , Al‐Sawalmeh Khaled , Al‐Bozom Issam , Hussein Mousa S. , Yassin Mohamed A. 
Published: July 2021

AbstractWhile evaluating the cause of Cushing's syndrome, biochemical confirmation should be sought first as imaging studies might misdirect the diagnosis toward the wrong problem. One of the rare secondary causes that should be kept in mind while evaluating Cushing's syndrome is the thymic neuroendocrine tumor.
Hydrolysis of Sucrose over Sulfonic Acid Resins
Authors: Buttersack Christoph , Hofmann Jörg , Gläser Roger 
Published: July 2021

AbstractSucrose hydrolysis on acidic ion‐exchange resins is a long‐established process in the sugar industry, but the formation of side products is less understood. A resin typically used in the industrial process (C124SH, Purolite) was investigated in a broad range of sucrose concentration (7 to 75 % w/w) and temperatures from 25 to 80 °C. Fructose is converted to 5‐hydroxymethylfurfural (HMF) due to a 1/2 order reaction. This is explained by the formation of a fructose disaccharide (monoanhydride) as an intermediate decomposing to HMF and fructose. Difructose dianhydrides (DFAs) are separately formed as prevailing by‐products. The color of the product mixture is correlated to HMF and found to be reduced by size exclusion inside the resins. Diffusional limitations of the total reaction are investigated by comparison of the entire resin bead with its size‐reduced forms obtained by milling. Without diffusion limitation the catalytic activity increases with the sugar concentration while the diffusion control leads to a maximal activity around 40 % w/w sugar. Diffusion coefficients of sucrose are calculated.
Characterization of Tbr2‐expressing retinal ganglion cells
Authors: Chen Ching‐Kang , Kiyama Takae , Weber Nicole , Whitaker Christopher M. , Pan Ping , Badea Tudor C. , Massey Stephen C. , Mao Chai‐An 
Published: July 2021

AbstractThe mammalian retina contains more than 40 retinal ganglion cell (RGC) subtypes based on their unique morphologies, functions, and molecular profiles. Among them, intrinsically photosensitive RGCs (ipRGCs) are the first specified RGC type emerging from a common retinal progenitor pool during development. Previous work has shown that T‐box transcription factor T‐brain 2 (Tbr2) is essential for the formation and maintenance of ipRGCs, and that Tbr2‐expressing RGCs activate Opn4 expression upon native ipRGC ablation, suggesting that Tbr2+ RGCs contain a reservoir for ipRGCs. However, the identity of Tbr2+ RGCs has not been fully vetted. Here, using genetic sparse labeling and single cell recording, we showed that Tbr2‐expressing retinal neurons include RGCs and a subset of GABAergic displaced amacrine cells (dACs). Most Tbr2+ RGCs are intrinsically photosensitive and morphologically resemble native ipRGCs with identical retinofugal projections. Tbr2+ RGCs also include a unique and rare Pou4f1‐expressing OFF RGC subtype. Using a loss‐of‐function strategy, we have further demonstrated that Tbr2 is essential for the survival of these RGCs and dACs, as well as maintaining the expression of Opn4. These data set a strong foundation to study how Tbr2 regulates ipRGC development and survival, as well as the expression of molecular machinery regulating intrinsic photosensitivity.
Peripheral blood stem cell mobilization with pegylated granulocyte colony stimulating factor in children
Authors: Thakkar Dhwanee , Tiwari Aseem K , Pabbi Swati , Kapoor Rohit , Aggarwal Geet , Rastogi Neha , Yadav Satya Prakash 
Published: July 2021

AbstractBackground and AimWe report here our experience of using pegylated granulocyte colony stimulating factor (peg‐GCSF) for peripheral blood stem cell (PBSC) mobilization in children.Methods and resultsA total of nine children suffering from high‐risk/relapsed solid tumors were mobilized with chemotherapy and peg‐GCSF (100 microgram/kg single dose). Mean age was 7.7 years (range 2–15 years).The mean time from peg‐GCSF administration to PBSC harvest was 9.7 days. Adequate stem cells (median dose 26.9 million/kg) could be harvested in all children by a single apheresis procedure. No major adverse events observed.ConclusionIt is feasible and safe to mobilize PBSC with peg‐GCSF in children with cancer.
Bridging the gap in outpatient care: Can a daily patient‐reported outcome measure help?
Authors: Meryk Andreas , Kropshofer Gabriele , Hetzer Benjamin , Riedl David , Lehmann Jens , Rumpold Gerhard , Haid Alexandra , Holzner Bernhard , Crazzolara Roman 
Published: July 2021

AbstractBackgroundChildhood patients have high risks for developing debilitating somatic and mental health side‐effects as a consequence of the many different approaches employed in treating their cancer. Early recognition and close monitoring of clinical and psychological problems are essential in planning appropriate interventions and preventing further deterioration.CaseePROtect was established as an easy‐to‐use application for daily self‐reporting of symptoms during cancer therapy. ePROtect includes six to eight questions pertaining to seven common symptoms: appetite loss, fatigue, nausea, pain, physical functioning, cognitive impairments and sleep quality. The case of a child diagnosed with Burkitt leukemia developing chemotherapy‐induced oral mucositis in home care is presented to show the therapeutic impact of early symptom detection with a daily web‐based tool.ConclusionThis case highlights how electronic patient‐reported outcome measures (PROM) can directly facilitate patient care in real time and might be incorporated in future clinical routine.
Full versus partial thickness flap to determine differentiation and over keratinization of non‐keratinized mucosa. A 3‐year split mouth randomized pilot study
Authors: Puisys Algirdas , Auzbikaviciute Viktorija , Vindasiute‐Narbute Egle , Zukauskas Saulius , Razukevicus Dainius , Dard Michel M. 
Published: July 2021

AbstractObjectivesIt was shown, that Connective Tissue Grafts (CTG) retrieved from the tuberosity tends to determine hyperplastic responses and may induce a beneficial over‐keratinization of non‐keratinized mucosa. Clinically evaluate and compare CTG from tuberosity ability to increase soft tissue thickness and the keratinization potential after recipient area is either prepared using split or full thickness flap in edentulous mandible.Materials and methodsFourty implants were placed in 10 edentulous patients with atrophied mandible (Class IV of Misch) presenting less than 1.0 mm of keratinized tissue using a flapless approach and immediately restored with acrylic temporary bridge on multi‐unit abutments. The surgical sites were split‐mouth randomized and prepared as CTG recipients by a tunneling procedure. Twenty benefited of a partial thickness approach and 20 of a full thickness one. The CTG was placed buccally using partial thickness or full thickness flap according to the randomization schedule. The width of keratinized tissue (KT), the horizontal soft tissue thickness (STT), the marginal hard and soft tissue levels as well as the implant success parameters were collected and analyzed.ResultsAfter a 3 year follow‐up period the increase of KT was statistically significantly (p < 0.001) larger in the partial thickness group from 0.6(0.6) to 5.1(0.72) mm, while full thickness group showed very little improvement from 0.5(0.51) to 1(0.57) mm (p < 0.001). STT was significantly increased in both groups over time: from 2.4(0.88) to 5.4(0.68) mm in full thickness group and from 2.5(0.51) to 5.8(0.41) mm in partial thickness group without any significant difference between the groups.ConclusionThe increase of soft tissue thickness by using CTG from tuberosity was found in both groups, while keratinization of non‐keratinized mucosa appeared more in the partial thickness group.