bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis
Authors: Hoffmann Timothy D. , Paine Kevin , Gebhard Susanne 
Published: November 2021

Abstract
Background
Microbially induced calcite precipitation (MICP) is an ancient property of bacteria, which has recently gained considerable attention for biotechnological applications. It occurs as a by-product of bacterial metabolism and involves a combination of chemical changes in the extracellular environment, e.g. pH increase, and presence of nucleation sites on the cell surface or extracellular substances produced by the bacteria. However, the molecular mechanisms underpinning MICP and the interplay between the contributing factors remain poorly understood, thus placing barriers to the full biotechnological and synthetic biology exploitation of bacterial biomineralisation.

Results
In this study, we adopted a bottom-up approach of systematically engineering Bacillus subtilis, which has no detectable intrinsic MICP activity, for biomineralisation. We showed that heterologous production of urease can induce MICP by local increases in extracellular pH, and this can be enhanced by co-expression of urease accessory genes for urea and nickel uptake, depending on environmental conditions. MICP can be strongly enhanced by biofilm-promoting conditions, which appeared to be mainly driven by production of exopolysaccharide, while the protein component of the biofilm matrix was dispensable. Attempts to modulate the cell surface charge of B. subtilis had surprisingly minor effects, and our results suggest this organism may intrinsically have a very negative cell surface, potentially predisposing it for MICP activity.

Conclusions
Our findings give insights into the molecular mechanisms driving MICP in an application-relevant chassis organism and the genetic elements that can be used to engineer de novo or enhanced biomineralisation. This study also highlights mutual influences between the genetic drivers and the chemical composition of the surrounding environment in determining the speed, spatial distribution and resulting mineral crystals of MICP. Taken together, these data pave the way for future rational design of synthetic precipitator strains optimised for specific applications.
Evaluation of pilot implementation of seasonal malaria chemoprevention on morbidity in young children in Northern Sahelian Ghana
Authors: Ansah Patrick O. , Ansah Nana A. , Malm Keziah , Awuni Dennis , Peprah Nana , Dassah Sylvester , Yarig Sobe , Manful Charles , Agbenyeri John , Awoonor-Williams John , Ofosu Wilfred , Oduro Abraham R. 
Published: November 2021

Abstract
Background
In Sahelian Africa, the risk of malaria increases with the arrival of the rains, particularly in young children. Following successful trials, the World Health Organization (WHO) recommended the use of seasonal malaria chemoprevention (SMC) in areas with seasonal peak in malaria cases. This study evaluated the pilot implementation of SMC in Northern Ghana.

Methods
Fourteen communities each serving as clusters were selected randomly from Lawra District of Upper West Region as intervention area and West Mamprusi District in the Northern Region as the non-intervention area. The intervention was undertaken by the National Malaria Control Programme in collaboration with regional health directorates using sulfadoxine-pyrimethamine plus amodiaquine and standard WHO protocols. Before and after surveys for malaria parasitaemia and haemoglobin levels as well as monitoring for malaria morbidity and mortality were undertaken.

Results
At the end of the intervention, participant retention was 92.9% (697/731) and 89.5% (634/708) in the intervention and the non-intervention areas, respectively. The proportion of children with asexual parasites reduced by 19% (p = 0.000) in the intervention and increased by 12% (p = 0.000) in the non-intervention area. Incidence rates of severe malaria were 10 and 20 per 1000 person-years follow up in the intervention and comparison areas, respectively with P.E of 45% (p = 0.62). For mild malaria, it was 220 and 170 per 1000 person-years in intervention and comparison area, respectively with PE of - 25% (p = 0.31). The proportion of children with anaemia defined as Hb< 11.0 g/dl reduced from 14.2% (52.8–38.6%) in the intervention area as compared to an increase of 8.1% (54.5% to 62.6) the non-intervention arm, Mean Hb reduced by 0. 24 g/dl (p = 0.000) in the non-intervention area and increased of 0.39 g/dl (p = 000) in the intervention area.

Conclusions
The feasibility and effectiveness of SMC introduction in Northern Ghana was demonstrated as evidenced by high study retention, reduction in malaria parasitaemia and anaemia during the wet season.
Circular RNAs in acute myeloid leukemia
Authors: Singh Vijendra , Uddin Mohammed Hafiz , Zonder Jeffrey A. , Azmi Asfar S. , Balasubramanian Suresh Kumar 
Published: November 2021

AbstractAlthough mechanistic studies clarifying the molecular underpinnings of AML have facilitated the development of several novel targeted therapeutics, most AML patients still relapse. Thus, overcoming the inherent and acquired resistance to current therapies remains an unsolved clinical problem. While current diagnostic modalities are primarily defined by gross morphology, cytogenetics, and to an extent, by deep targeted gene sequencing, there is an ongoing demand to identify newer diagnostic, therapeutic and prognostic biomarkers for AML. Recent interest in exploring the role of circular RNA (circRNA) in elucidating AML biology and therapy resistance has been promising. This review discerns the circular RNAs’ evolving role on the same scientific premise and attempts to identify its potential in managing AML.
Multifaceted glycoadjuvant@AuNPs inhibits tumor metastasis through promoting T cell activation and remodeling tumor microenvironment
Authors: Xu Xiaojing , Gan Minfeng , Ge Youzhen , Yi Cheng , Feng Tianyun , Liu Mengjie , Wu Cenhao , Chen Xiang , Zhang Weidong , Zhao Lixiang , Zou Jun 
Published: November 2021

Abstarct
Background
Cytosine-phosphate-guanine (CpG) dinucleotides has been used as adjuvants for cancer immunotherapy. However, unmodified CpG are not very efficient in clinical trials. Glucose, ligand of C-type lectin receptors (CLRs), can promote DC maturation and antigen presentation, which is the first step of induction of adaptive immune responses. Therefore, conjugation of type B CpG DNA to glucose-containing glycopolymers may enhance the therapeutic effects against tumor by CpG-based vaccine.

Methods
gCpG was developed by chemical conjugation of type B CpG DNA to glucose-containing glycopolymers. The therapeutic effects of gCpG-based vaccine were tested in both murine primary melanoma model and its metastasis model.

Results
gCpG based tumor vaccine inhibited both primary and metastasis of melanoma in mice which was dependent on CD8 + T cells and IFNγ. In tumor microenvironment, gCpG treatment increased Th1 and CTL infiltration, increased M1 macrophages, decreased Tregs and MDSCs populations, and promoted inflammatory milieu with enhanced secretion of IFNγ and TNFα. The anti-tumor efficacy of gCpG was dramatically enhanced when combined with anti-PD1 immunotherapy.

Conclusions
We confirmed that gCpG was a promising adjuvant for vaccine formulation by activating both tumor-specific Th1 and Tc1 responses, and regulating tumor microenvironments.

Graphical Abstract
Ten-year survival of ceramic-on-ceramic total hip arthroplasty in patients younger than 60 years: a systematic review and meta-analysis
Authors: El-Desouky Ihab Ibraheam , Helal Albaraa Hassan , Mansour Ali Mohamed Reda 
Published: November 2021

Abstract
Background
Total hip arthroplasty (THA) with ceramic-on-ceramic (CoC) was created to minimise wear debris and aseptic loosening. A decade ago, a meta-analysis showed a 10-year survival rate of just 89%. Based on the excellent tribology of the current CoC, significant improvement of implant survivorship is expected. In patients younger than 60, we conducted a meta-analysis to assess 10-year survival and complications after using current primary CoC THA.

Materials and methods
PubMed, Scopus, EMBASE, Virtual Health Library, and Cochrane Library were used to scan for published trials that met the inclusion criteria until January 2019. The qualified studies were subjected to a systematic review and proportional analysis, and the randomised controlled trials (RCTs) were included in a comparison meta-analysis.

Results
Thirteen studies were included 156 findings. The total number of hips was 2278. Nine studies were cohort, and four were RCTs between ceramic and polyethylene cups. The analysis revealed an average age of 44 years (range 24–54). The 10-year survival 96% (95% CI; 95.4–96.8%), aseptic loosening rate 0.516. (95% CI; 0.265–0.903), ceramic fracture rate 0.620 (95% CI; 0.34–1.034) and squeaking rate 2.687 (95% CI; 1.279–4.593). A comparison meta-analysis revealed the risk ratio (RR) for revision was 0.27 (95% CI; 0.15–0.47), and for aseptic loosening 0.15 (0.03–0.70) favouring CoC, while RR for component fracture was 1.62 (95% CI; 0.27–9.66) favouring the polyethylene.

Conclusion
In patients under sixty, current CoC THAs are correlated with better 10-year outcomes than before and have high survivorship rates.
Level of evidence: Level I.
Long noncoding RNA SNHG25 promotes the malignancy of endometrial cancer by sponging microRNA-497-5p and increasing FASN expression
Authors: He Yuhua , Xu Shuifang , Qi Yi , Tian Jinfang , Xu Fengying 
Published: November 2021

Abstract
Background
Small nucleolar RNA host gene 25 (SNHG25), a long noncoding RNA, has been well-studied in epithelial ovarian cancer. However, the specific functions of SNHG25 in endometrial cancer (EC) have not been studied yet. In this study, we aimed to elucidate the clinical significance of SNHG25 in EC and determine the regulatory activity of SNHG25 on the tumor-associated EC phenotype. We also thoroughly explored the molecular mechanisms underlying SNHG25 function in EC.

Methods
Gene expression was measured using quantitative real-time polymerase chain reaction. The detailed functions of SNHG25 in EC were examined by performing loss-of-function experiments. Moreover, the regulatory mechanisms involving SNHG25, microRNA-497-5p, and fatty acid synthase (FASN) were unveiled using the luciferase reporter assay and RNA immunoprecipitation.

Results
We observed a high level of SNHG25 in EC using the TCGA dataset and our study cohort. Patients with a high SNHG25 level had shorter overall survival than those with a low SNHG25 level. SNHG25 deficiency resulted in tumor-repressing activities in EC cells by decreasing cell proliferation, migration, and invasion and promoting cell apoptosis. Furthermore, the function of SNHG25 depletion in impairing tumor growth in vivo was confirmed. SNHG25 sequestered miR-497-5p as a competing endogenous RNA in EC and consequently positively regulated FASN expression. Thus, the decrease in miR-497-5p or increase in FASN could neutralize the modulatory actions of SNHG25 knockdown in EC cells.

Conclusions
The depletion of SNHG25 impedes the oncogenicity of EC by targeting the miR-497-5p/FASN axis. The newly elucidated SNHG25/miR-497-5p/FASN pathway may be a promising target for the molecular-targeted management of EC.
Gradual increasing dyslipidemia in treatment-naive male patients with human immunodeficiency virus and treated with tenofovir plus lamivudine plus efavirenz for 3 years
Authors: Liu Dafeng , Zhang Xinyi , Kang Jun , Gao Fengjiao , He Yinsheng , He Shenghua 
Published: November 2021

Abstract
Introduction
Since the development of antiretroviral therapy (ART) with TDF plus 3TC plus EFV, this specific regimen has not been studied enough with long-term lipid and uric acid monitoring.

Methods
A prospective follow-up cohort study was performed. Sixty-one treatment-naive male patients with human immunodeficiency virus (HIV) were divided into three groups based on their baseline CD4+ cell count (26, 12, and 23 patients in the < 200, 200 to 350, and > 350 groups, respectively). The lipid and purine metabolism parameters of the patients over 144 weeks were analyzed.

Result
Within 144 weeks, TG, LDL-c, TC and HDL-c gradually increased, especially TC and HDL-c (P = 0.001, 0.000, respectively). Moreover, the percentages of hyper-cholesterolemia, hyper LDL cholesterolemia, hyper-triglyceridemia and low HDL cholesterolemia also gradually increased, especially low HDL cholesterolemia significantly increased (P = 0.0007). The lower the baseline CD4+ cell counts were, the higher the TG levels and the lower the TC, LDL-c and HDL-c levels were. But there was significant difference of only baseline LDL-c levels between the three groups (P = 0.0457). No significant difference of the UA level and the percentages of hyperuricemia was found between the different follow-up time point groups or between the three CD4+ cell counts groups (all P > 0.05). The risk factors for dyslipidemia included age, anthropometric parameters and follow-up weeks, and for hyperuricemia was virus load.

Conclusions
Gradual increasing dyslipidemia was found in male patients with human immunodeficiency virus primarily treated with tenofovir plus lamivudine plus efavirenz for 3 years. There-fore lipid metabolism parameters should be closely monitored during long-term ART with the TDF plus 3TC plus EFV regimen.
Financial literacy among Finnish adolescents in PISA 2018: the role of financial learning and dispositional factors
Authors: Silinskas Gintautas , Ahonen Arto K. , Wilska Terhi-Anna 
Published: November 2021

AbstractThe aim or the present study was to examine the relative importance of financial education in school and families and dispositional factors (competitiveness, work mastery, meta-cognition) in predicting financial literacy among Finnish adolescents. The data on the 4328 Finnish 15-year-olds was drawn from the PISA 2018 assessment. Financial literacy was measured by tests, and financial education and dispositional factors were assessed by adolescent questionnaires. First, the results showed that financial education in school was positively associated with adolescents’ financial literacy skills, whereas parental involvement in financial matters did not relate or related negatively to financial literacy scores. Second, dispositional factors, such as competitiveness, work mastery, and meta-cognition (effective strategies to understand/remember information, to summarize information, and to evaluate source credibility) were the strongest positive predictors of the financial literacy scores. Overall, the present study emphasizes that certain social factors (schools and families) and especially dispositional characteristics (personality/motivation and critical thinking/learning strategies) may shape the development of the financial skills of adolescents.
Hypoglycaemic activity of Smilax canellifolia Mill. rhizomes: a bioassay-guided isolation and identification of synergistic compounds
Authors: Peddie Davia A. , Bryan Sophia J. , Francis Sheena , Alexander-Lindo Ruby L. 
Published: November 2021

Abstract
Background
Smilax canellifolia Mill. is a native shrub used in commercial root tonics as an aphrodisiac, stimulant, and pain reliever. Traditional medicine incorporates the rhizomes of S. canellifolia for the treatment of anaemia, rheumatoid arthritis, and diabetes in Jamaica and its diaspora. In particular, the use of this plant in the management of diabetes has yet to receive any scientific evaluation. In this study, the hexane crude extract of S. canellifolia rhizomes (SCH) was investigated to determine its hypoglycaemic activity in normal Sprague-Dawley rats and to identify the compounds contributing to this activity.

Methods
The hypoglycaemic compounds were isolated using bioactivity-guided purification which involved hypoglycaemic screening using an Oral Glucose Tolerance Test (via intravenous administration of SCH and its fractions). Purification was performed using column chromatography, and the bioactive fractions were elucidated using spectroscopic techniques (IR; GC-MS; 1H NMR and 13C NMR).

Results
Administration of SCH at 50 mg/kg body weight (BW) to normal S-D rats produced a reduced glycaemic response, notably from the 90 to the 150-min intervals when compared with the control, dimethyl sulfoxide (p < 0.05). Purification of this extract yielded four main fractions, SCH1 – SCH4, of which SCH3 and SCH4 displayed significant hypoglycaemia. Further purification of both SCH3 and SCH4 led to the isolation of sub-fractions SCH3.6 and SCH4.2, respectively. Using spectroscopic techniques stigmasterol (1) and β-sitosterol (2) from SCH3.6; and the fatty acids palmitic acid (3), oleic acid (4), and stearic acid (5) from SCH4.2 were identified as the major compounds with significant hypoglycaemic activities comparable to that of glibenclamide.

Conclusion
This study demonstrates that the rhizomes of Smilax canellifolia contain several bioactive constituents that are responsible for its hypoglycaemic activity and may be beneficial in the management of hyperglycaemia and complications associated with diabetes.
Superconducting energy storage technology-based synthetic inertia system control to enhance frequency dynamic performance in microgrids with high renewable penetration
Authors: Magdy Gaber , Bakeer Abualkasim , Alhasheem Mohammed 
Published: November 2021

AbstractWith high penetration of renewable energy sources (RESs) in modern power systems, system frequency becomes more prone to fluctuation as RESs do not naturally have inertial properties. A conventional energy storage system (ESS) based on a battery has been used to tackle the shortage in system inertia but has low and short-term power support during the disturbance. To address the issues, this paper proposes a new synthetic inertia control (SIC) design with a superconducting magnetic energy storage (SMES) system to mimic the necessary inertia power and damping properties in a short time and thereby regulate the microgrid (µG) frequency during disturbances. In addition, system frequency deviation is reduced by employing the proportional-integral (PI) controller with the proposed SIC system. The efficacy of the proposed SIC system is validated by comparison with the conventional ESS and SMES systems without using the PI controller, under various load/renewable perturbations, nonlinearities, and uncertainties. The simulation results highlight that the proposed system with SMES can efficiently manage several disturbances and high system uncertainty compared to the conventional ESS and SMES systems, without using the PI controller.
An unexpected sudden death due to a choroid plexus papilloma: an autopsy case report
Authors: Zaara Mohamed Amine , Gharsallaoui Sarra , Abdessayed Nihed , Mestiri Sarra , Chiba Dorra , Dhiab Mohamed Ben , Mokni Moncef 
Published: November 2021

AbstractBackgroundChoroid plexus papillomas (CPPs) are histopathologically benign and rare central nervous system tumors. These tumors remain more frequent in children than adults. It is infrequent for these tumors to cause a sudden unexpected death. We aim in this case to discuss the unusual and fatal presentation of choroid plexus papilloma and the mechanism of death.Case presentationwe report the case of a 61-year-old man with no medical history, diagnosed at autopsy with a previously unknown CPP. Initial complaints were chronic headache occurring in the last month and acute chest pain for two days. The forensic autopsy including the histopathologic examination showed a tumoral mass of the choroid plexus in the fourth ventricle diagnosed as a psammomatous CPP. The cause of death in this case was attributed to a massive cerebral edema caused by the tumoral mass.ConclusionsThrough this case report, we stress the importance of an early and a vigorous investigation of every headache and an early detection of this tumor and we highlight as well the role of the post mortem examination to detect such a fatal complication.
Randomized, Double-Blind, Phase III Study of Fosnetupitant Versus Fosaprepitant for Prevention of Highly Emetogenic Chemotherapy-Induced Nausea and Vomiting: CONSOLE
Authors: Hata Akito , Okamoto Isamu , Inui Naoki , Okada Morihito , Morise Masahiro , Akiyoshi Kohei , Takeda Masayuki , Watanabe Yasutaka , Sugawara Shunichi , Shinagawa Naofumi , Kubota Kaoru , Saeki Toshiaki , Tamura Tomohide 
Published: November 2021

PURPOSE We evaluated the efficacy and safety of fosnetupitant (FosNTP) versus fosaprepitant (FosAPR) for preventing highly emetogenic chemotherapy-induced nausea and vomiting. This phase III study was the first head-to-head comparison between two different neurokinin-1 receptor antagonists in combination with palonosetron and dexamethasone. PATIENTS AND METHODS Patients scheduled to receive cisplatin-based chemotherapy were randomly assigned 1:1 to FosNTP 235 mg or FosAPR 150 mg in combination with palonosetron 0.75 mg and dexamethasone. The primary end point was overall (0-120 hours) complete response (CR; no emetic event and no rescue medication) rate, stratified by sex and age category, to show the noninferiority of FosNTP to FosAPR (noninferiority margin, –10% for the difference in the overall CR rate). RESULTS Overall, 795 patients were randomly assigned, of whom 785 received the study drug (FosNTP [N = 392] v FosAPR [N = 393]) and were evaluated for efficacy and safety. The overall CR rate was 75.2% versus 71.0%, respectively (Mantel-Haenszel common risk difference, 4.1%; 95% CI, –2.1% to 10.3%), demonstrating noninferiority of FosNTP to FosAPR. The CR rates in the acute (0-24 hours), delayed (24-120 hours), and beyond delayed (120-168 hours) phases, and at 0-168 hours were 93.9% versus 92.6%, 76.8% versus 72.8%, 86.5% versus 81.4%, and 73.2% versus 66.9%, respectively. The incidence rates of treatment-related adverse events with FosNTP versus FosAPR were 22.2% versus 25.4%, whereas adverse events or treatment-related adverse events relevant to injection site reactions were 11.0% versus 20.6% ( P < .001) and 0.3% versus 3.6% ( P < .001), respectively. CONCLUSION FosNTP demonstrated noninferiority to FosAPR, with a favorable safety profile and lower risk for injection site reactions. Thus, FosNTP is valuable in the prophylaxis of acute, delayed, and beyond delayed chemotherapy-induced nausea and vomiting.
Influence of 3D printing stress wave on residual stress
Authors: Zhao Li , Jiang Zhaoliang , Zhang Cheng , Liu Wenping 
Published: November 2021

Abstract
As a popular rapid manufacturing technology, three-dimensional printing (3D printing) has been widely adopted in medical, automotive, aerospace, construction and other industrial fields. The stereo lithography apparatus (SLA) is an effective 3D printing method for ceramic printing. However, shrinkage from monomer to polymer and laser stress wave cause residual stress in the formed parts during SLA. Serious quality defects including cracks, warpage and deformation caused by residual stress have remained to be a problem. Basically, the laser stress wave plays an important role in the generation of 3D printing residual stress. In this work, to investigate the propagation mechanism of the laser stress wave, the finite element method was adopted to simulate the SLA process of zirconia. The influence of 3D printing factors on the residual stress was obtained, and we found that the wave velocity of the stress wave obtained by the simulation model was highly consistent with the theoretical wave velocity. Meanwhile, the attenuation formula of the stress wave in the 3D printing process was obtained by fitting to investigate its attenuation law. Based on the above results, the attenuation law of the 3D printing stress wave has a direct influence on the development and variation trend of its residual stress.