bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENINGKATAN KETERAMPILAN MENULIS PUISI DENGAN MENGGUNAKAN MODEL PEMBELAJARAN EXAMPLE DAN NON-EXAMPLE SISWA KELAS X SMA NEGERI 10 KOTA TERNATE
Authors: Sibua Sulami , Rahmadhany Firdha 
Published: February 2021

This research was conducted wiht the aim to find out how the students ability in writing poetry in class X SMAN 10 Ternate city and how to improve the ability to write poetry using the example and non-example models of grade X students of SMAN 10 ternate city. The methoud used in this research is descriptive quantitative and the instruments used are observation, interviews, tests and documentation.Based on the results of the studi, poetry writing skills improvement using example and non-example models class X students of SMAN 10 Ternate city consisting of 25 students can be seen that students are able writing poetry using model example and non-example, this is known from the results of research that there are worksheets on the pretest and postets which have increased, in the pretest and postest which have in creased, in the pretest with atotal value of 1.005 with and average value of 40,2 whereas the results of the posttest in the write poetry using the example and non-example models class X students of SMAN 10 Ternate city, with a value of 1.550 demgas an average value of 63,6 as manyÂ 23 students 68% have achieved (KKM) from the initial test results of 17 studens 92 % did not reach (KKM).
Influence of thermal processing on the characteristics and chemical profile of ora-pro-nobis by PS/MS paper spray
Authors: Souza Antonio Henrique de , Silva Bárbara Moreira , Silva Ernani Clarete da , Augusti Rodinei , Melo Júlio Onésio Ferreira , Carlos Lanamar de Almeida 
Published: February 2021

This work aimed to evaluate the influence of dry and wet heat processing on the antioxidant profile and antioxidant activity in ora-pro-nobis leaves. The leaves were collected, washed, and separated into three groups for the treatments: application of moist heat (hydrothermal cooking at 100 ºC / 4 minutes), application of dry heat (70 ºC / 8 hours), and control (raw material). The characteristics were evaluated: total soluble solids (SST), total solids (ST), pH, phenolic compounds (CPT), flavonoids (FT), anthocyanins (AT), antioxidant activity (AA). To identify the chemical profile and the chemical profile, fingerprints were obtained using PS / MS paper spray. The content of SST and ST decreased with moist heat. The CFT content increased with wet heat (12.43 ± 0.98) and decreased with dry heat (4.25 ± 0.93 g EAG 100 g-1). There was a decrease in the FT and AT content in the leaves processed with dry heat (132.67 ± 20.28 mg 100 g-1 and 19.70 ± 3.34 mg CG 100 g-1, respectively). AA decreased in both processes, which was higher in ora-pro-nobis leaves processed with dry heat (0.82 ± 0.0033 µmol ET g-1). 26 phenolic compounds were identified in the ora-pro-nob leaves by paper spray. The chemical profile, except for the substances 4,5-dimethyl-2,6-octadiene and methyl ester linolenic acid was not affected by thermal processing. Knowing the effect of food processing is important to understand the behavior of compounds that act beneficially on human health.
HUBUNGAN MASA PEMAKAIAN HIGH HEELS TERHADAP NYERI OTOT GASTROCNEMIUS PADA KARYAWAN SALES PROMOTION GIRL DI KOTA MALANG
Authors: Rahmanto Safun , Rahim Anita Faradilla , Salampessy Fini Aprilia Sitra 
Published: February 2021

Latar Belakang : Penggunaan high heels dengan tinggi diatas lima sentimeter, membuat posisi kaki dalam keadaan plantar fleksi. posisi ini dapat memicu terjadinya kontraksi pada otot gastrocnemius secara terus-menerus untuk mempertahankan posisi berdiri yang seimbang sehingga ketegangan pada serabut otot meningkat dan membuat otot gastrocnemius menjadi tegang sehingga menimbulkan rasa nyeri. Tujuan : Mengetahui hubungan masa pemakaian high heels terhadap nyeri otot gastrocnemius pada karyawan sales promotion girl di kota Malang. Metode Penelitian : Penelitian ini menggunakan metode observasional analitik dengan pendekatan cross sectional study. Responden dalam penelitian ini adalah karyawan sales promotion girl di Ramayana Dinoyo Malang, instrument yang di pakai adalah kuisioner nordic body maps, analisa data menggunakan uji Spearman. Hasil : Berdasarkan hasil uji spearman didapatkan nilai sig.(2-tailed) sebesar 0,010 sehingga lebih kecil dari alpha <0,05 dengan keterangan H0 ditolak dan H1 diterima. Dari hasil tersebut dapat disimpulkan bahwa ada hubungan masa pemakaian high heels terhadap nyeri otot gastrocnemius pada karyawan sales promotion girl di kota Malang. Kesimpulan : Terdapat hubungan masa pemakaian high heels terhadap nyeri otot gastrocnemius pada karyawan sales promotion girl di kota Malang.
Функціонування фундаментальної бібліотеки Київської духовної семінарії у другій половині ХІХ - на початку ХХ ст.
Authors: Бідун Юрій 
Published: February 2021

Досліджено важливу складову історії Київської духовної семінарії – функціонування її головної бібліотеки. З’ясовано, що попри науковий характер література книгосховища була вагомою частиною навчальної інфраструктури. Охарактеризовано особливості впорядкування літературних фондів в останній чверті ХІХ ст. Детально проаналізовано те, як змінювався склад і наповнення фундаментальної бібліотеки протягом другої половини ХІХ – початку ХХ ст. Виявлено, що якщо у 1880-х рр. в даному книгосховищі знаходилося 6 тис. книг у 12 тис. томах, то напередодні Першої світової війни тут вже було майже 12 тис. книг у 20 тис. томах. Звернено увагу на серйозну цензуру й обмеження книжкового обігу відомствами Св. Синоду. Показано особливості закупівлі та придбання літератури до бібліотеки.
Implementation of Complete Systematic Land Registration Regulation in order to Achieve Legal Certainty in Indonesia
Authors: Herdarezki Nadila Maysila , Handayani I Gusti Ayu Ketut Rachmi , Karjoko Lego 
Published: February 2021

This legal research aims to examine the implementation of regulations on the Complete Systematic Land Registration in order to achieve legal certainty in Indonesia. This legal research is a normative or doctrinal legal study conducted with a review of the literature. The research approach is conducted using statue approach and legal comparison approach (comparative law). This legal research uses both primary and secondary legal materials regarding the implementation of the Complete Systematic Land Registration in Indonesia. Based on the results of this legal research, implementation of the Complete Systematic Land Registration Program is a form of policy issued by the Indonesian Government with the Ministry of Agrarian Administration/National Land Agency to register land that has not owned a proprietary certificate. In order to achieve the legal certainty for the community is required improvement of good quality of public awareness, implementation mechanisms and various parties related to the Complete Systematic Land Registration in Indonesia.
Keywords: land; complete systematic land registration; legal certainty; policy
Reduced Severity in Patients With HIV-Associated Disseminated Histoplasmosis With Deep Lymphadenopathies: A Trench War Remains Within the Lymph Nodes?
Authors: Nacher Mathieu , Alsibai Kinan Drak , Adenis Antoine , Blaizot Romain , Abboud Philippe , Demar Magalie , Djossou Félix , Epelboin Loïc , Misslin Caroline , Ntab Balthazar , Valdes Audrey , Couppié Pierre 
Published: February 2021

BackgroundDisseminated histoplasmosis is a major killer of patients with advanced HIV. It is proteiform and often hard to diagnose in the absence of diagnostic tests. We aimed to describe disseminated histoplasmosis with lymphadenopathies in French Guiana and to compare survival and severity of those patients to patients without lymphadenopathies.MethodsA retrospective cohort study was performed on data records collected between January 1, 1981 and October 1, 2014.ResultsAmong 349 cases of disseminated histoplasmosis 168 (48.3%) had superficial lymphadenopathies and 133(38.1%) had deep lymphadenopathies. The median LDH concentration, ferritin concentration, TGO concentration, and WHO performance status were lower among patients with deep lymphadenopathies than those without deep lymphadenopathies. There was a significant decrease in the risk of early death (&lt;1 month) among those with deep lymphadenopathies relative to those without (OR=0.26 (95%CI=0.10–0.60), P=0.0006) and in the overall risk of death (OR=0.33 (95%CI=0.20-0.55), P&lt;0.0001). These associations remained strongly significant after adjusting for time period, CD4 counts, age, delay between beginning of symptoms and hospital admission, antifungal and antiretroviral treatment.ConclusionsThe present data show that in patients with advanced HIV and disseminated histoplasmosis, the presence of deep lymphadenopathies is associated with fewer markers of severity and a lower risk of death. To our knowledge it is the first study to show this. The presence of deep lymphadenopathies is hypothesized to reflect the patient’s partially effective defense against H. capsulatum.
Association of Genetic Polymorphisms in MicroRNAs With Type 2 Diabetes Mellitus in a Chinese Population
Authors: Zhu Zaihan , Zhang Yanfen , Bai Ruocen , Yang Ru , Shan Zhongyan , Ma Chunyan , Yang Jun , Sun Dandan 
Published: February 2021

IntroductionMicroRNAs (miRNA) involved in the insulin signaling pathways deeply affect the pathogenesis of T2DM. The aim of this study was to assess the association between single nucleotide polymorphisms (SNP) of the related miRNAs (let-7f rs10877887, let-7a-1 rs13293512, miR-133a-1 rs8089787, miR-133a-2 rs13040413, and miR-27a rs895819) and susceptibility to type 2 diabetes mellitus (T2DM), and its possible mechanisms.MethodsFive SNPs in miRNAs (let-7f rs10877887, let-7a-1 rs13293512, miR-133a-1 rs8089787, miR-133a-2 rs13040413, and miR-27a rs895819) involved in the insulin signaling pathways were selected and genotyped in a case-control study that enrolled 371 T2DM patients and 381 non-diabetic controls. The individual SNP association analyses, interaction analyses of SNP-SNP, SNP-environmental factors were performed. The effect the risk-associated polymorphism on regulating its mature miRNA expression was also evaluated.ResultsIn overall analyses, miR-133a-2 rs13040413 and let-7a-1 rs13293512 were related to the susceptibility to T2DM. In stratified analyses, miR-133a-2 rs13040413, let-7a-1 rs13293512 and miR-27a rs895819 showed associations with T2DM in the age ≥ 60 years subgroup. Moreover, let-7a-1 rs13293512 and miR-27a rs895819 showed associations with T2DM in male subgroup. In SNP-environmental factors interaction analyses, there were interaction effects of miR-133a-2 rs13040413 with dyslipidemia, let-7a-1 rs13293512 with smoking, and let-7a-1 rs13293512 with dyslipidemia on T2DM. In SNP-SNP interaction analyses, there were also interaction effects of miR-133a-1 rs8089787 with let-7a-1 rs13293512, and miR-133a-1 rs8089787 with let-7f rs10877887 on T2DM. Furthermore, for miR-133a-2 rs13040413, the variant T allele showed a trend toward decreased miR-133a expression in comparison with the wild C allele. For let-7a-1 rs13293512, the variant C allele expressed a lower let-7a compared to the wild T allele.ConclusionMiRNAs polymorphisms involved in the insulin signaling pathways and the interaction effects of SNP-SNP, SNP-environmental factors were related to T2DM susceptibility in a Chinese population.
Tissue MicroRNA Expression as a Predictor of Response to Immunotherapy in NSCLC Patients
Authors: Grenda Anna , Krawczyk Paweł , Błach Justyna , Chmielewska Izabela , Kubiatowski Tomasz , Kieszko Stanisław , Wojas-Krawczyk Kamila , Kucharczyk Tomasz , Jarosz Bożena , Paśnik Iwona , Borowiec-Bar Małgorzata , Frąk Małgorzata , Kieszko Robert , Szczyrek Michał , Reszka Katarzyna , Krukowska Kinga , Kolak Agnieszka , Mańdziuk Sławomir , Kowalski Dariusz , Sawicki Marek , Świniuch Daria , Starosławska Elżbieta , Ramlau Rodryg , Szumiło Justyna , Krzakowski Maciej , Milanowski Janusz 
Published: February 2021

IntroductionExpression of PD-L1 protein on tumor cells, which is so far the only validated predictive factor for immunotherapy, is regulated by epigenetic and genetic factors. Among the most important ones that regulate gene expression are microRNAs.Materials and MethodsThe study included 60 patients with NSCLC who underwent first or second line immunotherapy with pembrolizumab or nivolumab. FFPE materials were collected before the start of immunotherapy. We examined relative expression of microRNAs (miR-141, miR-200a, miR-200b, miR-200c, miR-429, miR-508-3p, miR-1184, miR-1255a) and PD-L1 mRNA expression. Copy number variation (CNV) of PD-L1 gene by qPCR and FISH methods were assessed. Two single nucleotide polymorphisms (SNPs) in promoter region of PD-L1 gene (rs822335 and rs822336) were examined. Expression of PD-L1 protein on tumor cells was assessed by immunohistochemistry (IHC). The response rate to immunotherapy and progression free survival (PFS) measured in weeks and overall survival (OS) measured in months from the start of immunotherapy were evaluated.ResultsResponse to immunotherapy was observed in nine patients (15%, including one complete response), disease stabilization in 22 patients (36.7%), and progression in 29 patients (48.3%). Significantly higher (p=0.015) expression of miR-200b and significantly lower (p=0.043) expression of miR-429 were observed in responders compared to patients who did not respond to immunotherapy. The median PFS in the whole group of patients was 16 weeks, and the median OS was 10.5 month. In univariate analysis, the median PFS was significantly higher in patients with high miR-200b expression (HR=0.4253, 95%CI: 0.1737–1.0417, p=0.05) and high miR-508 expression (HR=0.4401, 95%CI: 0.1903–1.0178, p=0.05) and with low expression of miR-429 (HR=0.1288, 95%CI: 0.01727–0.9606, p=0.0456) compared to patients with low and high expression of these molecules, respectively. The median OS was higher in patients with low expression of miR-429 (HR=0,6288, 95%CI: 0,3053–1,2949, p=0.06) compared with patients with high expression of this microRNA. In multivariate analysis, we found that patients with PD-L1 expression on ≥1% of tumor cells compared to patients without PD-L1 expression on cancer cells had a significantly lower risk of progression (HR=0.3857, 95%CI: 0.1612–0.9226, p=0.0323) and death (HR=0.377, 95%CI: 0.1636–0.8688, p=0.022).ConclusionThe miR-200b and miR-429 molecules in tumor cells seem to have greatest impact on the effectiveness of immunotherapy in NSCLC patients.
Prospective Exploratory Study of the Clinical Significance of Circulating Tumor Cells in Patients With Small Cell Lung Cancer Exposed to Prophylactic Cranial Irradiation
Authors: Deng Lei , Zhang Ye , Zhang Wen , Feng Lin , Zhang Kaitai , Wang Wenqing , Zhou Zongmei , Wang Luhua , Hui Zhouguang 
Published: February 2021

ObjectiveCirculating tumor cells (CTCs) can predict the efficacy of anti-cancer treatments and indicate prognosis. Here we investigate the significance of CTCs in relation to the prediction of treatment efficacy and prognosis in patients with small cell lung cancer (SCLC) who have received prophylactic cranial irradiation (PCI).MethodsCTCs were detected in 20 patients with SCLC before and after PCI using the oHSV1-hTERT-GFP method. The primary endpoints were progression-free survival (PFS) and overall survival (OS).ResultsEleven patients had limited-stage SCLC, and nine had extensive-stage SCLC. All patients completed chemo-radiotherapy and received PCI. The median baseline CTC count before PCI was 12. After PCI, the median CTC count was 4. The median follow-up time for all enrolled patients was 39.2 months. The median PFS and OS were significantly reduced in patients with ≥4 CTCs after PCI compared to those with &lt;4 CTCs (PFS, 28.1 months vs. not reached, p = 0.001; OS, not reached vs. not reached, p = 0.029). Seven of the 10 patients with ≥4 CTCs after PCI failed after treatment, whereas the10 patients with &lt;4 CTCs after PCI remained alive without tumors. The median PFS and OS were significantly improved in patients who exhibited a rate of CTC decline of ≥58% after PCI compared with patients who exhibited a decline rate of &lt;58% (PFS, 26.4 months vs. not reached, p = 0.006; OS, not reached vs. not reached, p = 0.029).ConclusionIn SCLC patients who receive PCI, the CTC count and rate of CTC decline after PCI significantly correlate with prognosis.
mTOR Inhibitors as Radiosensitizers in Neuroendocrine Neoplasms
Authors: Exner Samantha , Arrey Gerard , Prasad Vikas , Grötzinger Carsten 
Published: February 2021

Peptide receptor radioligand therapy (PRRT) has evolved as an important second-line treatment option in the management of inoperable and metastatic neuroendocrine neoplasms (NEN). Though high radiation doses can be delivered to the tumors, complete remission is still rare. Radiosensitization prior to PRRT is therefore considered to be a promising strategy to improve the treatment effect. In this study, effect and mechanism of mTOR inhibitors were investigated in a comprehensive panel of five NEN cell lines (BON, QGP-1, LCC-18, H727, UMC-11), employing assays for cellular proliferation, clonogenic survival, cell cycle modification and signaling. mTOR inhibition lead to growth arrest with a biphasic concentration-response pattern: a partial response at approximately 1 nM and full response at micromolar concentrations (8–48 µM). All cell lines demonstrated elevated p70S6K phosphorylation yet also increased phosphorylation of counterregulatory Akt. The pulmonary NEN cell line UMC-11 showed the lowest induction of phospho-Akt and strongest growth arrest by mTOR inhibitors. Radiation sensitivity of the cells (50% reduction versus control) was found to range between 4 and 8 Gy. Further, mTOR inhibition was employed together with irradiation to evaluate radiosensitizing effects of this combination treatment. mTOR inhibition was found to radiosensitize all five NEN cells in an additive manner with a moderate overall effect. The radiation-induced G2/M arrest was diminished under combination treatment, leading to an increased G1 arrest. Further investigation involving a suitable animal model as well as radioligand application such as177Lu-DOTATATE or177Lu-DOTATOC will have to demonstrate the full potential of this strategy for radiosensitization in NEN.
Screening and Identification of Four Prognostic Genes Related to Immune Infiltration and G-Protein Coupled Receptors Pathway in Lung Adenocarcinoma
Authors: Wang Yan , Qiu Liwei , Chen Yu , Zhang Xia , Yang Peng , Xu Feng 
Published: February 2021

BackgroundLung adenocarcinoma (LUAD) is a common malignant tumor with the highest morbidity and mortality worldwide. The degree of tumor immune infiltration and clinical prognosis depend on immune-related genes, but their interaction with the tumor immune microenvironment, the specific mechanism driving immune infiltration and their prognostic value are still not very clear. Therefore, the aim of this work was focused on the elucidation of these unclear aspects.MethodsTCGA LUAD samples were divided into three immune infiltration subtypes according to the single sample gene set enrichment analysis (ssGSEA), in which the associated gene modules and hub genes were screened by weighted correlation network analysis (WGCNA). Four key genes related to immune infiltration were found and screened by differential expression analysis, univariate prognostic analysis, and Lasso-COX regression, and their PPI network was constructed. Finally, a Nomogram model based on the four genes and tumor stages was constructed and confirmed in two GEO data sets.ResultsAmong the three subtypes—high, medium, and low immune infiltration subtype—the survival rate of the patients in the high one was higher than the rate in the other two subtypes. The four key genes related to LUAD immune infiltration subtypes were CD69, KLRB1, PLCB2, and P2RY13. The PPI network revealed that the downstream genes of the G-protein coupled receptors (GPCRs) pathway were activated by these four genes through the S1PR1. The risk score signature based on these four genes could distinguish high and low-risk LUAD patients with different prognosis. The Nomogram constructed by risk score and clinical tumor stage showed a good ability to predict the survival rate of LUAD patients. The universality and robustness of the Nomogram was confirmed by two GEO datasets.ConclusionsThe prognosis of LUAD patients could be predicted by the constructed risk score signature based on the four genes, making this score a potential independent biomarker. The screening, identification, and analysis of these four genes could contribute to the understanding of GPCRs and LUAD immune infiltration, thus guiding the formulation of more effective immunotherapeutic strategies.
Catalpol Protects Against Spinal Cord Injury in Mice Through Regulating MicroRNA-142-Mediated HMGB1/TLR4/NF-κB Signaling Pathway
Authors: Xia Hougang , Wang Dandan , Guo Xiaohui , Wu Kaidi , Huang Fuwei , Feng Yanjiang 
Published: February 2021

Background: Spinal cord injury (SCI) is a devastating condition that leads to paralysis, disability and even death in severe cases. Inflammation, apoptosis and oxidative stress in neurons are key pathogenic processes in SCI. Catalpol (CTP), an iridoid glycoside extracted from Rehmannia glutinosa, has many pharmacological activities, such as anti-inflammatory, anti-oxidative and anti-apoptotic properties.Purpose: Here, we investigated whether CTP could exert neuroprotective effects against SCI, and explored the underlying mechanism involved.Methods: SCI was induced by a weight-drop device and treated with CTP (10 mg and 60 mg/kg). Then the locomotor function of SCI mice was evaluated by the BBB scores, spinal cord edema was measured by the wet/dry weight method, oxidative stress markers and inflammatory factors were detected by commercial kits and neuronal death was measured by TUNEL staining. Moreover, the microRNA expression profile in spinal cords from mice following SCI was analyzed using miRNA microarray. In addition, reactive oxygen species (ROS) generation, inflammatory response and cell apoptosis were detected in murine microglia BV2 cells under oxygen-glucose deprivation (OGD) and CTPtreatment.Results: Our data showed that CTP treatment could improve the functional recovery, as well as suppress the apoptosis, alleviate inflammatory and oxidative response in SCI mice. In addition, CTP was found to be up-regulated miR-142 and the protective effects of CTP on apoptosis, inflammatory and oxidative response may relate to its regulation of HMGB1/TLR4/NF-κB pathway through miR-142.Conclusion: Our findings suggest that CTP may protect the spinal cord from SCI by suppression of apoptosis, oxidative stress and inflammatory response via miR-142/HMGB1/TLR4/NF-κB pathway.