bluuebunny/crossref_metadata_2025_split huggingface dataset:
Headache teleconsultation in the era of COVID‐19: Patients' evaluation and future directions
Authors: Dias Leonor , Martins Bárbara , Pinto Maria João , Rocha Ana Luísa , Pinto Madalena , Costa Andreia 
Published: May 2021

AbstractBackground and purposeLiterature regarding headache teleconsultation and patient satisfaction is scarce. The SARS‐CoV‐2 pandemic led to the restructuring of traditional clinical activity by adopting telemedicine. Our objectives were to evaluate patients' satisfaction with headache teleconsultation by telephone during the SARS‐CoV‐2 pandemic and assess patients' preferred model of appointment (face‐to‐face, teleconsultation by telephone, or both).MethodsPatients with a previous diagnosis of primary headache or neuropathies and facial pain disorders, and at least one telephone headache visit during the first wave of COVID‐19, filled out an online questionnaire regarding sociodemographic parameters, satisfaction with teleconsultation, and preferred model of appointment.ResultsWe included 83 patients (valid response rate of 64.3%); most had migraine (83.1%). Regarding teleconsultation, 81.9% considered this model adequate for follow‐up, 88.0% were satisfied with the information provided about the disease/treatment, and 73.5% were satisfied with the medication modification. Ninety percent would agree with a new tele‐evaluation if stable after the pandemic. The mixed model was the preferred medical consultation type for the postpandemic period (43.4%), followed by face‐to‐face visits (33.7%).ConclusionsPatients were satisfied with the headache teleconsultation during the COVID‐19 era. However, an exclusive model of telemedicine does not seem suitable for monitoring all patients. A mixed approach could be integrated into clinical practice after the pandemic to optimize health care.
P2X7 receptor/NLRP3 inflammasome complex and α‐synuclein in peripheral blood mononuclear cells: a prospective study in neo‐diagnosed, treatment‐naïve Parkinson’s disease
Authors: Solini Anna , Rossi Chiara , Santini Eleonora , Giuntini Martina , Raggi Francesco , Parolini Federico , Biancalana Edoardo , Del Prete Eleonora , Bonuccelli Ubaldo , Ceravolo Roberto 
Published: May 2021

AbstractBackground and purposeNeuroinflammation and probably systemic inflammation, with abnormal α‐synuclein deposition, participate in the development of Parkinson's disease (PD). The P2X7 receptor/NLRP3 inflammasome complex is upregulated in the brain of PD patients. By a prospective approach, the degree of systemic activation of such complex, and its regulatory mechanisms, were explored in treatment‐naïve PD individuals.MethodsThe expression and functional activity of the inflammasome were measured in peripheral blood mononuclear cells of 25 newly diagnosed PD patients and 25 controls at baseline and after 12 months of pharmacological treatment, exploring the intracellular signalling involved and its epigenetic regulation.ResultsDe novo PD patients were characterized by a systemic hyper‐expression of the P2X7R/NLRP3 inflammasome platform, probably able to modulate lymphomonocyte α‐synuclein, whose brain deposits represent the main pathogenetic factor of PD. A reduced c‐Jun N‐terminal kinase (JNK) phosphorylation might be the intracellular signalling mediating this effect. miR‐7 and miR‐30, implied in the pathogenesis of PD and in the post‐transcriptional control of α‐synuclein and NLRP3 expression, were also increased in PD. After 1 year of usual anti‐Parkinson treatments, such inflammatory platform was significantly reduced.ConclusionsMononuclear cells of newly diagnosed PD subjects display a hyper‐expression of the P2X7R/NLRP3 inflammasome platform that seems to modulate cellular α‐synuclein content and is reduced after PD treatment; an impaired JNK phosphorylation might be the intracellular signalling mediating this effect, undergoing an epigenetic regulation by miR‐7 and miR‐30.
H3F3A‐mutated giant cell tumour of bone without giant cells—clinical presentation, radiology and histology of three cases
Authors: Leinauer Benedikt , Wolf Eduard , Werner Mathias , Baumhoer Daniel , Breining Thomas , Luebke Andreas M , Maas Rainer , Schultheiß Markus , von Baer Alexandra , Sufi‐Siavach Anusch , Moritz Christian , Geißler Sven , Mellert Kevin , Möller Peter , Barth Thomas F E , Jundt Gernot 
Published: May 2021

AimsGiant cell tumour of bone (GCTB) is histologically defined as a lesion containing reactive giant cells and a neoplastic mononuclear cell population; in up to 92% of cases, GCTB is characterised by a specific mutation of the histone gene H3F3A. The cellular composition ranges from giant‐cell‐rich to giant‐cell‐poor. The diagnosis of GCTB can be challenging, and several other lesions need to be excluded, e.g. aneurysmal bone cysts, non‐ossifying fibromas, chondroblastomas, brown tumours, and giant‐cell‐rich osteosarcomas. Our aim was to analyse the clinical history, imaging, molecular pathology and histology of three H3F3A‐mutated bone tumours without detectable giant cells. None of the patients received denosumab therapy.Methods and resultsDiagnostic material was obtained by curettage or resection and/or biopsy. Common histomorphological features of all three reported lesions were fibrocytic, oval cells in a background of osteoid and an absence of multinuclear giant cells as confirmed with CD68 immunohistochemistry. We used immunohistochemistry and Sanger sequencing to demonstrate positivity for the H3.3 p.G34W mutation. Differential diagnoses were systematically excluded on the basis of histomorphology, immunohistochemistry, and fluorescence in‐situ hybridisation. The imaging (radiography, computed tomography, and magnetic resonance imaging) for all three cases is presented and discussed.ConclusionsWe believe that these GCTBs without giant cells expand one end of the heterogeneous range of GCTB. Because of the lack of giant cells, correct diagnosis of GCTB is challenging or even impossible on histological grounds alone. In these cases, detection of the characteristic H3F3A mutation (G34W‐specific antibody RM263 or sequencing) is extremely helpful for diagnosing those lesions without giant cells as giant cell tumours of bone.
Comparison of Bio‐C Pulpo and MTA Repair HP with White MTA: effect on liver parameters and evaluation of biocompatibility and bioactivity in rats
Authors: Delfino Mateus Machado , de Abreu Jampani José Leandro , Lopes Camila Soares , Guerreiro‐Tanomaru Juliane M. , Tanomaru‐Filho Mário , Sasso‐Cerri Estela , Cerri Paulo S. 
Published: May 2021

AbstractAimTo evaluate the tissue response promoted by Bio‐C Pulpo (Bio), MTA Repair HP (MTA‐HP) and White MTA (WMTA) and whether these materials cause liver changes in a rat experimental model.MethodologyPolyethylene tubes filled with Bio, MTA‐HP and WMTA, and empty tubes (control group, CG) were implanted into the subcutaneous tissues of rats for 7, 15, 30 and 60 days. Inflammatory reaction score (IRS), capsule thickness, number of inflammatory cells (IC), von Kossa reaction, interleukin‐6 (IL‐6) and alkaline phosphatase (ALP) immunohistochemistry reactions were performed. Combined methods, von Kossa followed by immunohistochemistry for detection of ALP, were performed. At 60 days, the serum glutamic‐oxaloacetic transaminase (GOT) and glutamic‐pyruvic transaminase (GPT) levels were measured and liver fragments were collected for histological analysis; the data were assessed by one‐way ANOVA analysis followed by Sidak's post‐test. The biocompatibility and bioactivity data were subjected to the two‐way ANOVA analysis followed by Tukey post hoc test, except the IRS. The IRS data were subjected to the Kruskal–Wallis ANOVA non‐parametric test followed by Dunn's test (p ≤ .05).ResultsNo significant difference was detected in serum GOT and GPT concentrations and in the number of hepatocytes among the experimental and CG samples. Although Bio‐C Pulpo had the highest IC and IL‐6‐immunolabelled cells (p < 0.0001) at all periods, no significant difference was observed in the IRS among the materials, except at 60 days. In this period, the WMTA had lower IRS. All groups had a significant reduction in the capsule thickness and in the number of IC and IL‐6‐immunolabelled cells over time. Bio‐C Pulpo, MTA‐HP and WMTA specimens had greater immunoexpression of ALP than CG (p < .0001). At all periods, von Kossa‐positive and birefringent structures were observed in the capsules around the materials. ALP‐immunolabelled cells were also seen near von Kossa‐positive structures.ConclusionsBio‐C Pulpo, MTA‐HP and WMTA materials did not cause morphological changes in the liver and no significant alteration in the serum GOT and GPT levels. Moreover, these bioceramic materials were biocompatible and exhibited bioactive potential. However, Bio‐C Pulpo induced greater inflammatory infiltrate than MTA‐HP and WMTA at all periods.
Impairment of the angiogenic process may contribute to lower success rate of root canal treatments in diabetes mellitus
Authors: Martinho José P. , Coelho Ana , Oliveiros Bárbara , Pires Salomé , Abrantes Ana Margarida , Paulo Siri , Carvalho Ana Catarina , Carrilho Eunice , Paula Anabela , Carvalho Lina , Seiça Raquel , Botelho Maria Filomena , Marto Carlos Miguel , Spagnuolo Gianrico , Matafome Paulo , Ferreira Manuel Marques 
Published: May 2021

AbstractAimTo investigate the association between root canal treatment outcome, diabetes mellitus, and alterations of the angiogenic process.MethodologyA retrospective observational study was conducted in healthy (control group, CG) and diabetic (type II diabetes mellitus group, DG) patients after root canal treatment. The follow‐up appointments were performed to clinically and radiographically observe symptoms, the healing of periapical lesions and the quality of root fillings. In the animal model study, diabetic Goto‐Kakizaki (GK) rats and control Wistar rats were used. After 21 days of pulp exposure and the development of apical periodontitis (AP), the mandibles were removed for scintigraphic, radiographic, histopathological and molecular analyses. Chi‐square tests were performed to examine the variables related to endodontic outcome and differences between animal groups were assessed using the Student's t‐test.ResultsThe group of patients with diabetes had a significantly lower rate of success following root canal treatment than the CG (p < .001). Logistic regression suggested that diabetes is a risk factor for success of root canal treatment. In the animal study, GK rats had significantly higher fasting glycaemia at t0 and t21 (p < .001) and triglycerides levels (p < .05) and area under the curve (AUC) during the insulin tolerance test at t21 (p < .001). AP area was significantly greater in GK rats (p < .05). Histologically, diabetic rats had increased signs of periodontal ligament inflammation 21 days after the induction of apical periodontitis, with fibro‐hyaline matrix filling and vessel with undefined walls. Wistar rats had significantly increased vascular endothelial growth factor (VEGF) levels and VEGF/Ang‐2 ratio 21 days after AP induction (p < .08; p < .07). GK rats had intrinsically lower levels of VEGF than control rats (p < .05), which did not change after AP.ConclusionDiabetes mellitus should be considered as an important factor in the prognosis of root canal treatment and its outcomes over time. Future strategies to improve angiogenesis and tissue repair should be pursued to achieve better root canal treatment outcomes in diabetic patients.
Predictors of worker mental health in intellectual disability services during COVID‐19
Authors: Lunsky Yona , Bobbette Nicole , Chacra Megan Abou , Wang Wei , Zhao Haoyu , Thomson Kendra , Hamdani Yani 
Published: May 2021

AbstractBackgroundWorkers supporting adults with intellectual disabilities experience significant stress in their essential role during COVID‐19. The purpose of this study was to describe the experience of these workers and determine predictors of emotional distress.MethodsEight hundred and thirty‐eight workers supporting adults with intellectual disabilities completed an online survey about their work during COVID‐19 and their mental health in July 2020.ResultsOne in four workers reported moderate to severe emotional distress. Being older and more experienced, having counselling services available through one's agency, and engaging in regular exercise or hobbies outside work were associated with less distress. Workers who reported increased stress in the workplace, stigma towards their families because of their job, personal fears about spreading COVID‐19, and receipt of medications for mental health conditions or therapy reported greater distress.ConclusionsMore attention is needed to address the mental health of workers supporting adults with intellectual disabilities as they continue their essential work during the pandemic.
Efforts targeted malnutrition among children with cerebral palsy in care homes and hospitals: A qualitative exploration study
Authors: Rosendahl Nanna Marie Hedegaard , Jensen Rikke Caroline , Holst Mette 
Published: May 2021

AbstractIntroductionChildren with cerebral palsy (CP) are at risk of becoming malnourished. Malnutrition risk increases with increased gross motor function classification. The present study aimed to investigate and explore existing nutritional efforts and practice among staff in Danish hospitals and care homes working with children with CP.MethodsTen qualitative interviews were generated with multidisciplinary staff working in hospital wards, care homes and Neuro Pediatric Team (NPT). Analysis was inspired by Braun and Clarke's thematic analysis.ResultsThe analysis generated the following six themes: Weighing and anthropometric measurements; Existing nutritional evaluation and intervention in practice; Cross‐sectoral communication and interdisciplinarity; Involvement of the children's parents; Knowledge and professional intuition; and Time and priority. Weighing is the most common nutritional evaluation, supplemented by weight/height curves in the NPT. No actual screening is performed. In care homes without healthcare staff, the nutritional focus primarily regards the meal and how the children are able to eat. In care homes with health staff, as well as hospital departments, the focus is primarily on weight. Written communication is a necessity for the nutritional collaboration around the child to work, but collaboration between IT‐systems are lacking. Interdisciplinarity and parental involvement are key components for managing nutrition issues among children with CP. The specialist dietitian was much required, but difficult to reach.ConclusionsDespite the great focus on nutrition and nutritional status among children with CP in all of the inquired contexts, existing efforts for assessment reveal primarily on weight and intuition. No actual screening is performed. Collaboration between sectors and professionals is challenged by lacking IT‐systems and systematic procedures.
In vivo method to evaluate antioxidative activity using UVA‐induced carbonylated protein on human skin
Authors: Cho Changhui , Lee Eunyoung , Cho Eunbyul , Yoo Heejoo , Bae Jiyoun , Ha Jaehyoun , Hwang Jaesung 
Published: May 2021

AbstractBackgroundSkin is continuously exposed to oxidative stress caused by reactive oxygen species (ROS) produced by the ultraviolet (UV) light, and it is important to evaluate the antioxidant activity. Carbonylated proteins (CPs) are candidate markers of oxidative modification as a result from the ROS. We aimed to develop the CP‐based method to assess the efficacy of antioxidants in human skin.MethodsTen healthy females were enrolled in the study to determine the UVA dosage for CP production, and another 10 females were included to evaluate the antioxidative activity. The stratum corneum was collected from test skin using D‐Squame tape, and CPs from the SC were stained by fluorescence labeling and observed using a fluorescence microscope.ResultsCP level significantly increased with UVA irradiation from 15J/cm2 to 50J/cm2 compared to the control (non‐UVA) area. CP production significantly increased by 34.38% and 35.22% in UVA irradiation and squalene (vehicle) areas. 5% α‐tocopherol and β‐carotene significantly increased the CP production by 20.77% and 19.34% after 2 hours of 30J of UVA irradiation compared to control area. Inhibition rate of CPs in 5% α‐tocopherol and 5% β‐carotene showed 41.45% and 45.37% after 2 hours of UVA irradiation.ConclusionThis study developed the simple, visual, and direct in vivo method to evaluate the antioxidative activity for products in human skin by measuring the CP level as an oxidative modification caused by UVA‐induced ROS generation.
Curvilinear relationship between emotional labour and work engagement in nurses: A correlational study
Authors: Yao Ying , Wei Wanhong , Hu Yanli , Li Yingying , Zhang Yanhui 
Published: May 2021

AbstractAims and ObjectivesTo examine if there was a curvilinear relationship between emotional labour and work engagement in Chinese nurses.BackgroundEmotional labour has both positive and negative effects on work engagement, but the curve relationship between the two has not been tested.DesignCorrelational design.MethodsMulti‐stage stratified random sampling was used to recruit samples, and 528 nurses from 5 tertiary first‐class general hospitals were recruited in Henan Province. Data were collected by using Hong and Kim's Emotional Labor Scale for nurse and the Utrecht Work Engagement Scale‐9. Curve estimation was used to explore the functional model between emotional labour and work engagement. Results were reported according to the STROBE Statement.ResultsThe three dimensions of emotional labour, ‘emotional control effort in profession’, ‘patient‐focused emotional suppression’ and ‘emotional pretense by norms’, had a function relationship of quadratic (R2 = .57, p < .001), quadratic (R2 = .569, p < .001) and cubic (R2 = .238, p < .001), respectively, with work engagement.ConclusionsThere was a one‐way increasing quadratic function relationship between ‘emotional control effort in profession’ and work engagement. However, ‘patient‐focused emotion suppression’ and ‘emotional pretense by norms’ had an inverted U‐shaped curve relationship with work engagement. Below the moderate level, ‘patient‐focused emotion suppression’ and ‘emotional pretense by norms’ were positively related to work engagement. After a certain level, they were negatively related to work engagement.Relevance to clinical practiceOur results may provide evidences to manage nurses’ emotional labour and improve their work engagement. It seems that encouraging or training nurses to learn and master more strategies of emotional control may improve work engagement. Moreover, nursing managers should fully recognise the positive and negative effects of different levels of emotional suppression and emotional pretense on work engagement, so as to develop targeted management strategies.
Physiological sleep measures predict time to 15‐year mortality in community adults: Application of a novel machine learning framework
Authors: Wallace Meredith L. , Coleman Timothy S. , Mentch Lucas K. , Buysse Daniel J. , Graves Jessica L. , Hagen Erika W. , Hall Martica H. , Stone Katie L. , Redline Susan , Peppard Paul E. 
Published: May 2021

SummaryClarifying whether physiological sleep measures predict mortality could inform risk screening; however, such investigations should account for complex and potentially non‐linear relationships among health risk factors. We aimed to establish the predictive utility of polysomnography (PSG)‐assessed sleep measures for mortality using a novel permutation random forest (PRF) machine learning framework. Data collected from the years 1995 to present are from the Sleep Heart Health Study (SHHS; n = 5,734) and the Wisconsin Sleep Cohort Study (WSCS; n = 1,015), and include initial assessments of sleep and health, and up to 15 years of follow‐up for all‐cause mortality. We applied PRF models to quantify the predictive abilities of 24 measures grouped into five domains: PSG‐assessed sleep (four measures), self‐reported sleep (three), health (eight), health behaviours (four), and sociodemographic factors (five). A 10‐fold repeated internal validation (WSCS and SHHS combined) and external validation (training in SHHS; testing in WSCS) were used to compute unbiased variable importance metrics and associated p values. We observed that health, sociodemographic factors, and PSG‐assessed sleep domains predicted mortality using both external validation and repeated internal validation. The PSG‐assessed sleep efficiency and the percentage of sleep time with oxygen saturation <90% were among the most predictive individual measures. Multivariable Cox regression also revealed the PSG‐assessed sleep domain to be predictive, with very low sleep efficiency and high hypoxaemia conferring the highest risk. These findings, coupled with the emergence of new low‐burden technologies for objectively assessing sleep and overnight oxygen saturation, suggest that consideration of physiological sleep measures may improve risk screening.
Accuracy of a flash glucose monitoring system in cats and determination of the time lag between blood glucose and interstitial glucose concentrations
Authors: Del Baldo Francesca , Fracassi Federico , Pires Jully , Tardo Antonio Maria , Malerba Eleonora , Manassero Elisa , Gilor Chen 
Published: May 2021

AbstractBackgroundThe FreeStyle Libre (Abbott Laboratories) is a flash glucose monitoring system (FGMS) that measures interstitial glucose concentration (IG). The system is factory‐calibrated, easy to use, inexpensive, and could be useful for monitoring diabetic cats.ObjectivesTo evaluate the analytical and clinical accuracy of the FGMS in cats and establish the lag‐time between IG and blood glucose concentration (BG).AnimalsTwenty client‐owned diabetic cats and 7 purpose‐bred healthy cats.MethodsProspective study. Blood glucose concentration was measured using a portable glucose meter validated for use in cats that served as a reference method for IG, as measured by FGMS. In diabetic cats, data were collected for sensor wearing time with different methods of application and accuracy across glycemic ranges. Accuracy was determined by fulfillment of ISO15197:2013 criteria. In healthy cats, lag‐time between IG and BG was established after IV administration of exogenous glucose.ResultsGood agreement between IG and BG was obtained (r = .93). Analytical accuracy was not achieved, whereas clinical accuracy was demonstrated with 100% of the results in zones A + B of the Parkes consensus error grid analysis. In the immediate 30 minutes after an IV bolus of glucose, when BG was increasing rapidly (approximately 2%/min), IG increased slowly, resulting in a difference of as much as 579 mg/dL, and no positive correlation between BG and IG was found.Conclusions and Clinical ImportanceThe FGMS did not fulfill ISO requirements but is sufficiently accurate for glucose monitoring in cats, while considering the lag between IG and BG during periods of rapid changes in BG.