bluuebunny/crossref_metadata_2025_split huggingface dataset:
Covid-19 vaccine-induced antibodies are attenuated and decay rapidly in infliximab treated patients
Authors: Lin Simeng , Kennedy Nicholas A , Saifuddin Aamir , Sandoval Diana Muñoz , Reynolds Catherine , Seoane Rocio Castro , Kottoor Sherine , Pieper Franziska , Lin Kai-Min , Butler David K , Chanchlani Neil , Nice Rachel , Chee Desmond , Bewshea Claire , Janjua Malik , McDonald Timothy J , Sebastian Shaji , Alexander James L , Constable Laura , Lee James C , Murray Charles D , Hart Ailsa L , Irving Peter M , Jones Gareth-Rhys , Kok Klaartje B , Lamb Christopher A , Lees Charlie W , Altmann Daniel M , Boyton Rosemary J , Goodhand James R , Powell Nick , Ahmad Tariq 
Published: July 2021

Abstract
To inform healthcare policy for immunosuppressed patients there is a need to define SARS-CoV-2 vaccine responses. Here we report SARS-CoV-2 vaccine-induced antibody and T cell responses in patients treated with anti-tumour necrosis factor (anti-TNF), a commonly used biologic in inflammatory diseases, compared to patients treated with vedolizumab, a gut-specific antibody targeting integrin a4b7 that does not impair systemic immunity. In anti-TNF recipients, the magnitude of anti-SARS-CoV2 antibodies was reduced five-fold, and rapidly decayed towards the seroconversion threshold by 14 weeks after second dose of vaccine. In contrast, anti-SARS-CoV-2 antibodies were sustained up to 16 weeks in vedolizumab-treated patients. Anti-SARS-CoV2 antibody decay was not observed in vaccinated patients previously infected with SARS-CoV-2. T cell responses were absent in one-fifth of anti-TNF and vedolizumab-treated patients after a second dose of either vaccine. Our data have important implications for anti-TNF recipients, including the need for vaccine prioritization, booster doses, and social distancing strategies.
Experimental analysis on the optimal excitation wavelength for fine-grained identification of refined oil pollutants on water surface based on laser-induced fluorescence
Authors: Xie Ming , Jia Yunpeng , Li Ying , Cai Xiaohua , Cao Kai 
Published: July 2021

Abstract
Laser-induced fluorescence (LIF) is an effective, all-weather oil spill identification method that has been widely applied for oil spill monitoring. However, the distinguishability on oil types is seldom considered while selecting excitation wavelength. This study is intended to find the optimal excitation wavelength for fine-grained classification of refined oil pollutants using LIF by comparing the distinguishability of fluorometric spectra under various excitation wavelengths on some typical types of refined-oil samples. The results show that the fluorometric spectra of oil samples significantly vary under different excitation wavelengths, and the four types of oil applied in this study are most likely to be distinguished under the excitation wavelengths of 395 nm and 420 nm. This study is expected to improve the ability of oil types identification using LIF method without increasing time or other cost, and also provides theoretical basis for the development of portable LIF devices for oil spill identification.
Hepatobiliary disease in XLMTM. A common comorbidity with potential impact on treatment strategies
Authors: D'Amico Adele , Longo Antonella , Fattori Fabiana , Tosi Michele , Bosco Luca , Testa Maria Beatrice Chiarini , Paglietti Giovanna , Cherchi Claudio , Carlesi Adelina , Mizzoni Irene , Bertini Enrico 
Published: July 2021

Abstract
BACKGROUND: X-linked myotubular myopathy (XLMTM) is a rare congenital myopathy resulting from pathogenic variants in the MTM1 gene. Affected male subjects typically present with severe hypotonia and respiratory distress at birth and they often require intensive supportive care. Long-term survivors are often non-ambulant, ventilator and feeding tube–dependent and they generally show additional organ manifestations, indicating that myotubularin does play a vital role in tissues other than muscle. For XLMTM several therapeutic strategies are under investigation.A recent clinical trial delivering the MTM1 gene using a recombinant AAV8 vector to XLMTM patients showed encouraging results in term of efficacy, but some concerns about safety arised due to three fatal events related to hepatotoxicity in boys that were exposed to the higher dose.RESULTS: We report prospective and retrospective clinical data of 12 XLMTM patients collected over a period of up to 10 years. In particular, we carried out a thorough review of the data about incidence and the course of hepatobiliary disease in our case series.CONCLUSIONS: We demonstrate that hepatobiliary disease represents a common comorbidity of XLMTM that seems irrespective to age and diseases severity. We recommend to carefully explore and monitor the hepatobiliary function in XLMTM patients. We believe that a better understanding of the pathogenic mechanisms that induce hepatobiliary damage is essential to understand the fatal events that may occur in the gene therapy program.
RIG-I acts as a tumor suppressor in melanoma via regulating the activation of MKK/p38MAPK signaling pathway
Authors: Guo Rui , Lu Shun-Yuan , Ma Jin-Xia , Wang Qian-Lan , Zhang Lu , Tang Ling-Yun , Shen Yan , Shen Chun-Ling , Wang Jin-Jin , Lu Li-Ming , Wang Zhu-Gang , Zhang Hong-Xin 
Published: July 2021

Abstract
Background
Studies have indicated that RIG-I may act as a tumor suppressor and participate in the tumorigenesis of some malignant diseases. However, RIG-I induces distinct cellular responses via different downstream signaling pathways depending on the cell type. The aim of this study was to investigate the biological function and underlying molecular mechanism of RIG-I in the tumorigenesis of melanoma.
Methods
We conducted RIG-I knockout and RIG-I overexpressing B16-F10 melanoma cell line, and further analyzed the RIG-I mediated change of tumor biology behaviors in spontaneous and poly I:C induced RIG-I activation status. Cell proliferation, cell cycle, apoptosis and migration were detected by CCK-8 assay, BrdU incorporation, Annexin V-PI staining assay and transwell assay, respectively. In vivo tumorigenicity was evaluated by tumor xenograft growth in nude mice and subsequently Ki67 staining and TUNEL assay. Furthermore, Western blot was utilized to explore the underlying mechanism of RIG-I in melanoma cells.
Results
Our data showed that RIG-I promotes the apoptosis and inhibits the proliferation by G1 phase cell-cycle arrest in B16-F10 melanoma cell line. Mechanically, RIG-I could induce the phosphorylation level of p38 MAPK and MAPK kinase MKK3/MKK4.
Conclusion
The current study demonstrated that RIG-I suppressed the development of melanoma via regulating the activity of MKK/p38 MAPK signaling pathway, which will be useful in the research of novel therapeutic targets for this malignant disease.
Raman Spectroscopic and Electrochemical Measurements of Dynamic Shocked MnFe2O4 Nano-Crystalline Materials
Authors: Sivakumar A. , Dhas S.Sahaya Jude , Sivaprakash P. , Almansour Abdulrahman I. , Kumar Raju Suresh , Arumugam Natarajan , Perumal Karthikeyan , Arumugam S. , Dhas Martin Britto 
Published: July 2021

Abstract
Divalent ferrite nano-crystalline materials subjected to shock wave recovery analysis have received non-debatable attention among the materials scientists due to their outstanding contribution that has fueled the enrichment of high-pressure ferrites science which act as the focal-point of the current research theme. On those lines, we intend to provide the Raman spectroscopic and electrochemical measurements of manganese ferrite nanoparticles (MnFe2O4 NPs) at dynamic shocked conditions. Raman spectroscopic analysis has revealed the stability of the tetrahedral (A-site) and octahedral (B-sites) sites of Mn2+ ions as well as Fe3+ ions at shocked conditions. Cyclic-voltammetry (CV-analysis) and impedance spectroscopic measurements of shocked samples have demonstrated the electro-chemical properties of MnFe2O4 NPs whose electrical conductivity is found to be reduced while increasing the number of shock pulses. The consolidated results provide the solid evidence that the test sample’s crystal structure does not undergo any crystallographic phase transitions or mixed spinel states at shocked conditions.
Phänotypisierung - Gesetzesentwurf eines grundrechtlich kontroversen Ermittlungsinstruments
Authors: Lehmann Julia 
Published: July 2021

Mittels Phänotypisierung werden aus DNA-Spuren Rückschlüsse auf das Erscheinungsbild einer Person gezogen. Künftig soll die Phänotypisierung als Ermittlungsinstrument im Strafprozess eingesetzt werden. Gemäss dem Gesetzesentwurf könnte die Staatsanwaltschaft die Phänotypisierung zur Aufklärung von Verbrechen anordnen. Dabei sollen die Augen-, Haar- und Hautfarbe sowie die biogeografische Herkunft und das biologische Alter ausgewertet werden können. Die Phänotypisierung an sich und die Auswertung dieser Merkmale durch die Strafverfolgungsbehörden sind umstritten. Mit dem Einsatz dieses neuen Ermittlungsinstruments drohen Verletzungen von Grundrechten, beispielsweise durch Racial Profiling, Diskriminierungen aufgrund von Äusserlichkeiten und aufgrund von Datenschutzverletzungen. Dieser Gefahr ist bereits bei der Gesetzgebung Rechnung zu tragen. Der Grundrechtsschutz im Gesetzesentwurf sollte daher im Hinblick auf die befürchteten Verletzungen verbessert werden.
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Le phénotypage permet de tirer des conclusions sur l'apparence d'une personne à partir de traces d'ADN. À l'avenir, le phénotypage sera utilisé comme outil d'investigation dans les procédures pénales. Selon le projet de loi, le ministère public pourrait ordonner le phénotypage pour résoudre des crimes. Ce faisant, la couleur des yeux, des cheveux et de la peau ainsi que l'origine biogéographique et l'âge biologique doivent pouvoir être évalués. Le phénotypage lui-même et l'évaluation de ces caractéristiques par les services de répression sont controversés. L'utilisation de ce nouvel outil d'investigation menace de violer les droits fondamentaux, par exemple par le profilage racial, la discrimination fondée sur l'apparence et les violations de la protection des données. Ce danger doit déjà être pris en compte dans la législation. La protection des droits fondamentaux dans le projet de loi devrait donc être améliorée au regard des violations redoutées.
Yeast race effect on the quality of base and young sparkling wines
Authors: Makarov Alexander , Lutkov Igor 
Published: July 2021

Introduction. A disadvantage of the ancestral method (la méthode ancestrale), which is widely used in the production of sparkling wine, is that it is difficult to control fermentation. We aimed to identify the optimal yeast race for obtaining high-quality young sparkling wines with varietal aroma without yeast tones.
Study objects and methods. Our study objects were base and young sparkling wines from Cabernet-Sauvignon prepared on various yeast races. Organic acids, sugars, and ethanol contents were determined by high performance liquid chromatography. Phenolic and coloring substances were measured by colorimetric method. Foaming properties were determined by air barbotage of a wine sample in a measuring cylinder; sparkling properties, by measuring the CO2 desorption rate; CO2 content, by volumetric method; viscosity, with a viscometer. Sensory evaluation was carried out according to standard methods.
Results and discussion. The wines produced on the Odesskiy Chernyi-SD13 yeast race received the highest tasting scores of 7.82 and 9.05 points for base wine and young sparkling wines, respectively. They contained larger amounts of phenolic substances (1103 mg/dm3) and coloring agents (275 mg/dm3) and had higher color intensity (1.614). The panelists rated them highly on their complex varietal aroma and harmonious, velvety flavor, as well as their foaming and sparkling properties. This yeast race ensured intensive fermentation of sugars and a great amount of bound CO2 (up to 24.93%).
Conclusion. The Odesskiy Chernyi-SD13 yeast race is optimal for making base and young sparkling wines by the bottle method. This technology can be used to produce high-quality sparkling wines in the crop year by large and small enterprises.
Unveiling the Role of Cytochrome P450 (2E1) in Human Brain Specifically in Parkinson’s Disease - Literature Review
Authors: Shah Amna , Ong Chin Eng , Pan Yan 
Published: July 2021

Background:
In recent years, the significance of cytochrome P450 enzymes (CYPs) has expanded beyond
their role in the liver. Factors such as genetics, environmental toxins, drug biotransformation and underlying diseases
mediate the expression of these enzymes. Among the CYP enzymes, CYP2E1, a well-recognized monooxygenase
enzyme involved in the metabolism of various endogenous and exogenous substances, plays a crucial role in the
brain concerning the development of Parkinson’s disease. The expression of CYP2E1 varies in different brain regions
making certain regions more vulnerable than others. CYP2E1 expression is inducible which generates tissuedamaging
radicals leading to oxidative stress, mitochondrial dysfunction and ultimately neurodegeneration.

Objective:
Less is understood about the role of CYP2E1 in the central nervous system, therefore the purpose of the
study was to investigate the relationship between the expression and activity of CYP2E1 enzyme relevant to Parkinson’s
disease and to identify whether an increase in the expression of CYP2E1 is associated with neurodegeneration.

Methods:
The objectives of the study were achieved by implicating an unsystematic integrative literature review
approach in which the literature was qualitatively analysed, critically evaluated and a new theory with an overall
view of the mechanism was presented.

Results:
The contribution of CYP2E1 in the development of Parkinson’s disease was found to be significant as the
negative effects of CYP2E1 overshadowed its protective detoxifying role.

Conclusion:
Overexpression of CYP2E1 seems detrimental to dopaminergic neurons, therefore, to overcome this, a
synthetic biochemical is required, which paves the way for further research and development of valuable biomolecules.
Sulfonamide-Functionalized Polymeric Nanoparticles for Enhanced In
Vivo Colorectal Cancer Therapy
Authors: Sinisterra Rubén D. , Guimarães Pedro Pires Goulart , Viana Celso Tarso Rodrigues , Pereira Luciana , Gontijo Savio Morato Lacerda , Campos Paula Peixoto , Andrade Silvia Passos , Santos Robson A.S. 
Published: July 2021

Background:
Colorectal cancer (CRC) is the third most common cancer in the world. 5-
Fluorouracil (5-FU) is a conventional and most effective drug used in the clinic for the treatment of
CRC. However, the clinical use of 5-FU is limited due to the acquired resistance and systemic
toxicity, such as hepatotoxicity and gastrointestinal toxicity.

Objective:
Recent advances in nanomedicine are being exploited to develop nanoparticle platforms
to overcome resistance and therapeutic delivery of active molecules. Here, we developed 5-FU
loaded sulfadiazine-poly(lactide-co-glycolide) nanoparticles (SUL-PLGA NPs) to be applied in the
colorectal cancer model.

Methods:
We assessed the in vivo efficacy of the SUL-PLGA NPs to enhance the antitumor effect
of 5-FU.

Results:
In vivo treatment with 5-FU-SUL-PLGA NPs significantly reduced tumor growth in a
colon cancer xenograft model compared to free 5-FU and 5-FU loaded non-targeted NPs. Treatment
with 5-FU-SUL-PLGA NPs also increased blood vessel diameters within tumors, which
could act in conjunction to enhance antitumor efficacy. In addition, 5-FU-SUL-PLGA NPs significantly
reduced liver mass and lung mass, which are the most common metastasis sites of CRC, and
decreased liver hepatotoxicity compared to free 5-FU drug and 5-FU loaded non-targeted NPs.

Conclusion:
Our findings suggest that the use of 5-FU-SUL-PLGA NPs is a promising strategy to
enhance 5-FU efficacy against CRC.
Ablation Therapy for Persistent Atrial Fibrillation
Authors: Ifedili Ikechukwu , Mouksian Kristina , Jones David , El Masri Ibrahim , Heckle Mark , Jefferies John , Levine Yehoshua C. 
Published: July 2021

:
Atrial Fibrillation (AF) is the most common form of electrical disturbance of the heart
and contributes to significant patient morbidity and mortality. With a better understanding of the
mechanisms of atrial fibrillation and improvements in mapping and ablation technologies, ablation
has become a preferred therapy for patients with symptomatic AF. Pulmonary Vein Isolation (PVI)
is the cornerstone for AF ablation therapy, but particularly in patients with AF occurring for longer
than 7 days (persistent AF), identifying clinically significant nonpulmonary vein targets and achieving
durability of ablation lesions remains an important challenge.
Resuscitating Cardiopulmonary Resuscitation Training in a Virtual Reality: Prospective Interventional Study
Authors: Perron Janaya Elizabeth , Coffey Michael Jonathon , Lovell-Simons Andrew , Dominguez Luis , King Mark E , Ooi Chee Y 
Published: July 2021

Background
Simulation-based technologies are emerging to enhance medical education in the digital era. However, there is limited data for the use of virtual reality simulation in pediatric medical education. We developed Virtual Doc as a highly immersive virtual reality simulation to teach pediatric cardiopulmonary resuscitation skills to medical students.

Objective
The primary objectives of this study were to evaluate participant satisfaction and perceived educational efficacy of Virtual Doc. The secondary aim of this study was to assess the game play features of Virtual Doc.

Methods
We conducted a prospective closed beta-testing study at the University of New South Wales (Sydney, Australia) in 2018. All medical students from the 6-year undergraduate program were eligible to participate and were recruited through voluntary convenience sampling. Participants attended a 1-hour testing session and attempted at least one full resuscitation case using the virtual reality simulator. Following this, participants were asked to complete an anonymous postsession questionnaire. Responses were analyzed using descriptive statistics.

Results
A total of 26 participants were recruited, consented to participate in this study, and attended a 1-hour in-person closed beta-testing session, and 88% (23/26) of participants completed the anonymous questionnaire and were included in this study. Regarding participant satisfaction, Virtual Doc was enjoyed by 91% (21/23) of participants, with 74% (17/23) intending to recommend the simulation to a colleague and 66% (15/23) intending to recommend the simulation to a friend. In assessment of the perceived educational value of Virtual Doc, 70% (16/23) of participants agreed they had an improved understanding of cardiopulmonary resuscitation, and 78% (18/23) agreed that Virtual Doc will help prepare for and deal with real-life clinical scenarios. Furthermore, 91% (21/23) of participants agreed with the development of additional Virtual Doc cases as beneficial for learning. An evaluation of the game play features as our secondary objective revealed that 70% (16/23) of participants agreed with ease in understanding how to use Virtual Doc, and 74% (17/23) found the game play elements useful in understanding cardiopulmonary resuscitation. One-third (7/23, 30%) found it easy to work with the interactive elements. In addition, 74% (17/23) were interested in interacting with other students within the simulation.

Conclusions
Our study demonstrates a positive response regarding trainee satisfaction and perceived educational efficacy of Virtual Doc. The simulation was widely accepted by the majority of users and may have the potential to improve educational learning objectives.
App-Tailoring Requirements to Increase Stress Management Competencies Within Families: Cross-sectional Survey Study
Authors: Bischoff Laura Luise , Baumann Hannes , Meixner Charlotte , Nixon Patricia , Wollesen Bettina 
Published: July 2021

Background
Families experiencing high levels of psychological distress are considered a particularly vulnerable population for adverse effects on mental and physical health. Moreover, highly stressed individuals engage less in mental health promoting activities and show low stress management competencies. App-based stress interventions seem promising for the treatment and prevention of stress outcomes and might be a low-threshold solution.

Objective
The aim of this study was to identify the requirements for a tailored app to reduce stress in a cohort of highly stressed families that have low stress management skills.

Methods
Parents (n=1008; age: mean 47.7 years, SD 6.1; female: 599/1008, 59.7%) completed an extensive web-based survey and were subdivided into a target (stressed individuals with low stress competency) and nontarget group according to their reported stress level and stress management competencies. Group differences were analyzed using analysis of variance. In principal component analysis with Kaiser varimax rotation, personally defined stress management goals were grouped into components. Linear regression models were also calculated.

Results
A 3-factor solution cumulatively explained 56% of the variance in personally defined goals of interest for stress management with (1) active strategies (25.61% explained variance), (2) general competency (17.95% explained variance) and (3) passive strategies (12.45% explained variance). The groups differed in age (F1,978=27.67, P<.001), health index (F1,958=246.14, P<.001), personally defined general-competency goal (F1,958=94.16 P<.001), as well as “information acquisition” (F1,971=14.75, P<.001) and “need for stimulation” (F1,981=54.49, P<.001) personality traits. A regression model showed that for the active strategies goals of interest, only app feature information or instructional videos had a significant effect (P=.02). The general competency factor showed none, and the passive strategies factor showed significant effects for 2 app features—suggestions for planning possible activities with the family (P=.01) and diaries for documentation and development of strategies (P=.03).

Conclusions
The results of this survey study highlight the need to develop an app to increase stress management competencies that takes into consideration perceived stress level, stress management skills, personality, and personally defined goals of the user. The content of the app should be tailored to previously detected personality traits, especially selective information acquisition and low need for stimulation. Furthermore, personally defined stress management goals seem to affect interest in some features.