bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological and numerical simulation of rabies spreading from canines to various human populations in mainland China
Authors: Lu Wen-gao , Ai Danni , Song Hong , Xie Yuan , Liu Shuqing , Zhu Wuyang , Yang Jian 
Published: July 2021

Background
The mortality of humans due to rabies in China has been declining in recent years, but it is still a significant public health problem. According to the global framework, China strives to achieve the goal of eliminating human rabies before 2030.

Methods
We reviewed the epidemiology of human deaths from rabies in mainland China from 2004 to 2018. We identified high risk regions, age and occupational groups, and used a continuous deterministic susceptibility-exposure-infection-recovery (SEIR) model with periodic transmission rate to explore seasonal rabies prevalence in different human populations. The SEIR model was used to simulate the data of human deaths from rabies reported by the Chinese Center for Disease Control and Prevention (China CDC). We calculated the relative transmission intensity of rabies from canines to different human groups, and they provided a reliable epidemiological basis for further control and prevention of human rabies.

Results
Results showed that human deaths from rabies exhibited regional differences and seasonal characteristics in mainland China. The annual human death from rabies in different regions, age groups and occupational groups decreased steadily across time. Nevertheless, the decreasing rates and the calculated R0s of canines of various human groups were different. The transmission intensity of rabies from canines to human populations was the highest in the central regions of China, in people over 45 years old, and in farmers.

Conclusions
Although the annual cases of human deaths from rabies have decreased steadily since 2007, the proportion of human deaths from rabies varies with region, age, gender, and occupation. Further enhancement of public awareness and immunization status in high-risk population groups and blocking the transmission routes of rabies from canines to humans are necessary. The concept of One Health should be abided and human, animal, and environmental health should be considered simultaneously to achieve the goal of eradicating human rabies before 2030.
Field assessment in Cameroon of a reader of POC-CCA lateral flow strips for the quantification of Schistosoma mansoni circulating cathodic antigen in urine
Authors: Mewamba Estelle Mezajou , Tiofack Arnol Auvaker Zebaze , Kamdem Cyrille Nguemnang , Ngassam Romuald Isaka Kamwa , Mbagnia Mureille Carole Tchami , Nyangiri Oscar , Noyes Harry , Womeni Hilaire Marcaire , Njiokou Flobert , Simo Gustave 
Published: July 2021

Background
Determining Schistosoma mansoni infection rate and intensity is challenging due to the low sensitivity of the Kato-Katz (KK) test that underestimates the true disease prevalence. Circulating cathodic antigen (CCA) excreted in urine is constantly produced by adult worms and has been used as the basis of a simple, non-invasive point of care test (POC-CCA) for Schistosoma mansoni infections. Although the abundance of CCA in urine is proportional to worm burden, the POC-CCA test is marketed as a qualitative test, making it difficult to investigate the wide range of infection intensities. This study was designed to compare the prevalence and intensity of S. mansoni by KK and POC-CCA and quantify, on fresh and frozen (<-20°C) urine samples, CCA using the visual scores and the ESEquant LR3 reader.

Methodology
Stool and urine samples were collected from 759 school-aged children. The prevalence and intensity of S. mansoni were determined using KK and POC-CCA. The degree of the positivity of POC-CCA was estimated by quantifying CCA on fresh and frozen urine samples using visual scores and strip reader. The prevalence, the infection intensity as well the relative amounts of CCA were compared.

Results
The S. mansoni infection rates inferred from POC-CCA and KK were 40.7% and 9.4% respectively. Good correlations were observed between infection intensities recorded by; i) the reader and visual scoring system on fresh (Rho = 0.89) and frozen samples (Rho = 0.97), ii) the reader on fresh urine samples and KK (epg) (Rho = 0.44). Nevertheless, 238 POC-CCA positive children were negative for KK, and sixteen of them had high levels of CCA. The correlation between results from the reader on fresh and frozen samples was good (Rho = 0.85). On frozen samples, CCA was not detected in 55 samples that were positive in fresh urine samples.

Conclusion
This study confirmed the low sensitivity of KK and the high capacity of POC-CCA to provide reliable data on the prevalence and intensity of S. mansoni infections. The lateral flow reader enabled accurate quantification of CCA under field conditions on fresh and frozen urine samples with less time and effort than KK.
Knowledge, beliefs and practices regarding prevention of bacterial meningitis in Burkina Faso, 5 years after MenAfriVac mass campaigns
Authors: Mueller Judith E. , Seanehia Joy , Yaro Seydou , Trotter Caroline L. , Borrow Ray , Giles-Vernick Tamara 
Published: July 2021

Background
To adapt communications concerning vaccine prevention, we studied knowledge, beliefs and practices around meningitis risk and prevention in a young adult population in Burkina Faso in 2016, 5 years after the MenAfriVac® mass campaign and one year before the vaccine’s inclusion in the infant immunization schedule.

Methods
In a representative sample of the population aged 15 to 33 years (N = 220) in Bobo-Dioulasso, Burkina Faso, study nurses administered a standardized paper questionnaire consisting of predominantly open questions, collecting information on meningitis risk factors and prevention, and on exposure to dry air and kitchen fire smoke. We identified themes and analyzed their frequency. We created a meningitis knowledge score (range 0 to 4) based on pre-defined best responses and analyzed the determinants of knowledge score levels ≥2 (basic score) and ≥3 (high score) using multivariate logistic regression.

Results
Biomedically supported facts and good practices were known by the majority of participants (eg vaccine prevention, 84.5%). Younger women aged 15–20 years had a higher frequency of low scores <2 (17.0%) compared to older women aged 21–33 years (6.3%) and men of both age groups (3.8%). Junior secondary School attendance explained the differences between the two groups of women, the gender gap for the older, but not the young women, and explained score differences among young women. Local understandings and practices for risk and prevention were commonly reported and used (risk from unripe mango consumption and prevention through nasal application of shea nut butter).

Discussion
This study shows a gender gap in knowledge of meningitis risk and prevention, largely due to education-level inequalities. Women below 21 years had particularly low levels of knowledge and may need interventions outside schools and perinatal care. Our study suggests a strong adherence to local understandings of and practices around meningitis risk and prevention, which should be taken into account by vaccination promotion.
Clinicopathological features of different subtypes in adenomyosis: Focus on early lesions
Authors: Kobayashi Hiroshi , Matsubara Sho , Imanaka Shogo 
Published: July 2021

Background
The aim of this study is to investigate the clinicopathological features of intrinsic and extrinsic subtypes in adenomyosis. In particular, we focused on the early lesions of adenomyosis.

Methods
This is a single-center, prospective study of women who elected surgery for adenomyosis at the Department of Gynecology, Nara Medical University Hospital, Kashihara, Japan, from April 2008 to March 2018. Adenomyosis was histologically classified as intrinsic, extrinsic, and others, depending on the type of intramural growth. Adenomyosis that occurs at the inner and outer myometrium was defined as an intrinsic and extrinsic type, respectively.

Results
One hundred eighty-nine patients with histologically confirmed adenomyosis were classified into three different types, 74 intrinsic type, 78 extrinsic type, and 37 other type. Compared to the intrinsic type, the extrinsic type was more likely to have endometriosis, including ovarian endometrioma (OMA), superficial peritoneal endometriosis (SUP), or deep infiltrating endometriosis (DIE). To further identify the clinicopathological features of early-stage adenomyosis, we focused only on patients with intrinsic and extrinsic types of adenomyosis with less than one-third of muscular layer infiltration. Patients with early-stage intrinsic adenomyosis were more likely to experience induced abortions. Patients with early-stage extrinsic adenomyosis were more likely to have endometriosis. The coexistence of endometriosis and the lack of induced abortion were independent predictors of extrinsic adenomyosis. Multivariate logistic regression analysis identified coexistence of endometriosis as independent predictors of the early stage extrinsic adenomyosis.

Conclusion
The study suggests that there are at least two types of adenomyosis, where the intrinsic type is closely associated with a history of induced abortion, while the extrinsic type is strongly associated with endometriosis. Adenomyosis might be a gynecological disorder with complex pathogenesis implicating both traumatic and endometriotic factors.
The impact of vaccination to control COVID-19 burden in the United States: A simulation modeling approach
Authors: Alagoz Oguzhan , Sethi Ajay K. , Patterson Brian W. , Churpek Matthew , Alhanaee Ghalib , Scaria Elizabeth , Safdar Nasia 
Published: July 2021

Introduction
Vaccination programs aim to control the COVID-19 pandemic. However, the relative impacts of vaccine coverage, effectiveness, and capacity in the context of nonpharmaceutical interventions such as mask use and physical distancing on the spread of SARS-CoV-2 are unclear. Our objective was to examine the impact of vaccination on the control of SARS-CoV-2 using our previously developed agent-based simulation model.

Methods
We applied our agent-based model to replicate COVID-19-related events in 1) Dane County, Wisconsin; 2) Milwaukee metropolitan area, Wisconsin; 3) New York City (NYC). We evaluated the impact of vaccination considering the proportion of the population vaccinated, probability that a vaccinated individual gains immunity, vaccination capacity, and adherence to nonpharmaceutical interventions. We estimated the timing of pandemic control, defined as the date after which only a small number of new cases occur.

Results
The timing of pandemic control depends highly on vaccination coverage, effectiveness, and adherence to nonpharmaceutical interventions. In Dane County and Milwaukee, if 50% of the population is vaccinated with a daily vaccination capacity of 0.25% of the population, vaccine effectiveness of 90%, and the adherence to nonpharmaceutical interventions is 60%, controlled spread could be achieved by June 2021 versus October 2021 in Dane County and November 2021 in Milwaukee without vaccine.

Discussion
In controlling the spread of SARS-CoV-2, the impact of vaccination varies widely depending not only on effectiveness and coverage, but also concurrent adherence to nonpharmaceutical interventions.
A novel algorithm for 3-D visualization of electrogram duration for substrate-mapping in patients with ischemic heart disease and ventricular tachycardia
Authors: Masjedi Mustafa , Jungen Christiane , Kuklik Pawel , Alken Fares-Alexander , Kahle Ann-Kathrin , Klatt Niklas , Scherschel Katharina , Lorenz Jürgen , Meyer Christian 
Published: July 2021

Background
Myocardial slow conduction is a cornerstone of ventricular tachycardia (VT). Prolonged electrogram (EGM) duration is a useful surrogate parameter and manual annotation of EGM characteristics are widely used during catheter-based ablation of the arrhythmogenic substrate. However, this remains time-consuming and prone to inter-operator variability. We aimed to develop an algorithm for 3-D visualization of EGM duration relative to the 17-segment American Heart Association model.

Methods
To calculate and visualize EGM duration, in sinus rhythm acquired high-density maps of patients with ischemic cardiomyopathy undergoing substrate-based VT ablation using a 64-mini polar basket-catheter with low noise of 0.01 mV were analyzed. Using a custom developed algorithm based on standard deviation and threshold, the relationship between EGM duration, endocardial voltage and ablation areas was studied by creating 17-segment 3-D models and 2-D polar plots.

Results
140,508 EGMs from 272 segments (n = 16 patients, 94% male, age: 66±2.4, ejection fraction: 31±2%) were studied and 3-D visualization of EGM duration was performed. Analysis of signal processing parameters revealed that a 40 ms sliding SD-window, 15% SD-threshold and >70 ms EGM duration cutoff was chosen based on diagnostic odds ratio of 12.77 to visualize rapidly prolonged EGM durations. EGMs > 70 ms matched to 99% of areas within dense scar (<0.2 mV), in 95% of zones within scar border zone (0.2–1.0 mV) and detected ablated areas having resulted in non-inducibility at the end of the procedure. Ablation targets were identified with a sensitivity of 65.6% and a specificity of 94.6% avoiding false positive labeling of prolonged EGMs in segments with healthy myocardium.

Conclusion
The novel algorithm allows rapid visualization of prolonged EGM durations. This may facilitate more objective characterization of arrhythmogenic substrate in patients with ischemic cardiomyopathy.
Preventing and managing antimicrobial resistance in the African region: A scoping review protocol
Authors: Iwu-Jaja Chinwe Juliana , Jaca Anelisa , Jaja Ishmael Festus , Jordan Portia , Bhengu Phelele , Iwu Chidozie Declan , Okeibunor Joseph , Karamagi Humphrey , Tumusiime Prosper , Fuller Walter , Yahaya Ali Ahmed , Wiysonge Charles , Gahimbare Laetitia 
Published: July 2021

Introduction
Antimicrobial resistance (AMR) constitutes a significant threat to global health and food security, typically associated with high morbidity and mortality rate. The high burden of infectious diseases coupled with the weak health systems in most countries of Africa magnifies the risk of increasing AMR and its consequences thereof. This scoping review will be aimed at mapping the evidence on interventions used to prevent and manage antimicrobial resistance in Africa, guided by the “One Health” concept.

Methods
We will consider interventions targeting multiple sectors such as health care systems, the agricultural and veterinary sectors. The outcomes to be considered include reduction of AMR decreased morbidity and mortality due to infectious diseases, increased awareness for rational use of antimicrobials and reduced antibiotic consumption. We will include all types of studies regardless of study designs conducted within the context of the WHO African region. Studies will be excluded if they are not conducted in Africa and if they are literature reviews, only describing the concept of AMR without mentioning interventions.
We will include studies identified through a comprehensive search of peer-reviewed and grey literature databases. In addition, we will search the reference lists of included studies and relevant reviews. Finally, we plan to do a citation search for included studies. Findings of this review will be narratively synthesized.
Identifikasi Molekuler Kapang Asosiasi Spons menggunakan Metode DNA Barcoding
Authors: Larasati Stefanie Jessica Henny , Sabdono Agus , Sibero Mada Triandala 
Published: July 2021

Spons merupakan organisme yang memiliki pori-pori dan termasuk kedalam filum Porifera. Hewan ini merupakan filter feeders dimana spons menyaring makanannya masuk kedalam rongga tubuhnya, sehingga spons dapan memakan partikel organik algae, dan mikroba, termasuk kapang. Kapang merupakan mikroorganisme eukariotik dari kingdom fungi, multiseluler, menghasilkan miselium tanpa pembentukan badan buah. Kapang dapat berfungsi sebagai penjaga keseimbangan ekosistem di perairan. Tujuan dari penelitian ini adalah mengidentifikasi dua isolat kapang yang telah diisolasi dari inang spons di ekosistem mangrove dengan menggunakan DNA barcoding. Metode dalam penelitian ini yaitu peremajaan isolat, karakterisasi morfologi yaitu warna koloni, tekstur, reverse, exudates, sclerotia, bentuk konidia, konidiofor, spora, dan septa. Identifikasi molekuler dari ekstraksi DNA, amplifikasi, elektroforesis, visualisasi DNA, sekuens dan BLAST. Optimasi suhu annealing dilakukan pada amplifikasi DNA. Berdasarkan identifikasi molekuler dengan menggunakan primer universal ITS1 5' TCCGTAGGTGAACCTGCGG 3' dan ITS4 5' TCCTCCGCTTATTGATATGC 3' dan persamaan homologi, isolat MKMS 2.1 merupakan Trichoderma reesei (100%) dan PKMS 2.2 merupakan spesies Fusarium solani (99,81%). A sponge is an organism that has pores and belongs to the Porifera phylum. These animals are filter feeders where the sponge filters its food into the body cavity, so the sponge can eat organic algae particles, and microbes, including fungi. Mold is a eukaryotic microorganism from Fungi kingdom, multicellular, that forms mycelium without fruiting body formation. Mold has an important role in balancing the environmental quality in an ecosystem. The purpose of this study was to identify two molds that had been isolated from sponge in the mangrove ecosystem using DNA barcoding. The study was conducted in April-October 2019 in Laboratory of Tropical Marine Biotechnology using the experimental laboratory method. The methods in this research were isolation refreshment, morphological characterization which were consisted of colony color, texture, reverse, exudates, sclerotia, conidia, conidiophores, spores, and septa. Molecular identification consisted of DNA extraction, amplification, electrophoresis, DNA visualization, sequences and BLAST. Annealing temperature optimization is carried out on DNA amplification. Based on molecular identification using universal primers ITS1 5 'TCCGTAGGTGAACCTGCGG 3' and ITS4 5 'TCCTCCGCTTATTGATATGC 3' and homological equations, MKMS 2.1 isolates were identified as Trichoderma reesei (100%) and PKMS 2.2 were identified as Fusarium solani (99.81%).
Kajian Morfometri Rajungan (Portunus pelagicus) Linnaeus, 1758 (Crustacea: Portunidae) pada Dua Fase Bulan yang Berbeda di Perairan Desa Tunggulsari, Rembang
Authors: Pratiwi Wukir Berliana , Nuraini Ria Azizah Tri , Widianingsih Widianingsih 
Published: July 2021

Fase bulan secara periodik mengalami perubahan dan menjadi faktor yang mempengaruhi perairan secara fisika, kimia, maupun biologi. Kondisi tersebut di wilayah perairan laut menimbulkan fenomena pasang surut yang memiliki pengaruh besar terhadap kehidupan biota laut seperti rajungan (Portunus pelagicus). Strategi penangkapan yang tepat dapat dilakukan dengan menentukan target satu spesies atau dengan cara melihat fase bulan. Penelitian ini bertujuan untuk melakukan kajian morfometri, nisbah kelamin, hubungan lebar berat, dan tingkat kematangan gonad rajungan (Portunus pelagicus) yang tertangkap nelayan pada fase bulan purnama dan bulan mati dari Perairan Desa Tunggulsari, Rembang. Pengambilan data dilakukan terhadap rajungan (Portunus pelagicus) yang tertangkap oleh nelayan dari perairan Desa Tunggulsari. Kegiatan penelitian dilakukan pada tanggal 9 Januari 2020, 28 Januari 2020, 10 Februari 2020, 25 Februari 2020, 10 Maret 2020, dan 24 Maret 2020. Hasil penelitian berdasarkan uji T statistik menunjukkan bahwa ada perbedaan komposisi rajungan dari fase bulan purnama dan bulan mati dengan selisih yang tidak signifikan. Rajungan yang tertangkap pada fase bulan purnama memiliki rata-rata ukuran berat dan lebar yang lebih kecil dibandingkan pada fase bulan mati. Analisis power curve hubungan lebar dan berat rajungan yang ada di perairan Desa Tunggulsari bersifat allometrik negatif yang berarti pertumbuhan lebar karapas lebih cepat dibandingkan beratnya. The lunar phase periodically changes and becomes a factor that affects the waters physically, chemically, and biologically. These conditions in the marine waters cause tidal phenomena which have a major influence on marine life such as blue swimming crab (Portunus pelagicus). The right fishing strategy can be done by determining the target of one species or by looking at the phases of the moon. This study aims to study the morphometry, sex ratio, weight-width relationship, and maturity level of the crab gonads (Portunus pelagicus) caught by fishermen in the full moon and new moon phases of the Tunggulsari Waters, Rembang. The method used in this research is descriptive exploratory. Research activities were carried out on January 9, 2020, January 28, 2020, February 10, 2020, February 25, 2020, March 10, 2020, and March 24, 2020. The results of the study based on the statistical T test showed that there was an insignificant difference in the catch of the full moon and new moon phases. The crabs that are caught in the full moon phase have an average size of weight and width that is smaller than in the new moon phase. Analysis of the relationship between the width and weight of small crabs in the waters of Tunggulsari Waters is negative allometric, which means that the carapace width grows faster than its weight.
Perubahan Jalur Pelayaran Terhadap Peta Perekonomian Asia Tenggara Dampak Pembangunan Terusan Kra Thailand
Authors: Persada Aninditya Gita Kireina , Setyawanta Lazarus Tri 
Published: July 2021

Terusan Kra merupakan kanal yang telah direncanakan pembangunannya oleh Thailand sejak abad ke-17. Dengan dibangunnya terusan Kra yang dimungkinkan berhasil memberi efisiensi terhadap penyingkatan waktu pelayaran di daerah Asia Pasifik tentu akan memberi dampak kerugian yang signifikan pada terusan Malaka yang telah terlebih dahulu dibuat. Dampak pembangunan tersebut akan mempengaruhi perubahan jalur pelayaran serta merubah peta perekonomian Asia Tenggara secara makro. Tujuan dari tulisan ini ada untuk mengetahui dampak dan perubahan signifikan terhadap pembangunan terusan Kra di masa depan. Metode yang digunakan merupakan pendekatan yuridis normatif yang menggunakan data sekunder melalui penelitian kepustakaan dan studi dokumen. Adanya terusan Kra akan merubah neraca ekspor dan impor secara signifikan serta akan memberikan perubahan besar ke beberapa negara-negara di Asia Pasifik. Hingga saat ini mulai banyak negara-negara di Asia Tenggara yang mulai merencanakan dan membangun kawasan pantainya agar dapat bersaing saat nantinya Terusan Kra dibuka. The Kra Canal is a canal that Thailand has planned to build since the 17th century. With the construction of the Kra canal, which is possible to provide efficiency in reducing shipping time in the Asia Pacific region, it will certainly have a significant impact on the Malacca canal that was previously built. This development impact will affect changes in shipping lanes and change the economic map of Southeast Asia at a macro level. The purpose of this paper is to determine the impact and significant changes to the development of the Kra canal in the future. The method used is a normative juridical approach that uses secondary data through library research and document study. The existence of the Kra canal will change the export and import balance significantly and will provide big changes for several countries in Asia Pacific. Until now, many countries in Southeast Asia have started planning and building their coastal areas so that they can compete when the Kra Canal opens.