bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influences of Intravenous Thrombolysis with Recombinant Tissue-type Plasminogen Activator on the Outcome of Atrial Fibrillation Patients with Acute Ischemic Stroke: A Case a Case-control Study of 227 Patients
Authors: Wang Hai-xia , Zhang Nan , Wang Guo-Qiang , Huang Yong-hua 
Published: June 2021

Abstract
Background: It is a debatable topic about the benefit of intravenous (IV) thrombolysis with recombinant tissue-type plasminogen activator (rt-PA) for atrial fibrillation (AF) patients with acute ischemic stroke (AIS). This study aimed to identify whether IV rt-PA could improve the short-term outcome of patients with AF-AIS.Methods: Medical data of patients with AIS onset within 72hs admitted in the department of neurologic of our hospital between January 1st, 2015 and December 31, 2020 were extracted. The AF-AIS patients were selected and divided into IV rt-PA group (group A) and non-rt-PA group (group B). The baseline characteristics, imaging changes and modified Rankin Scale (mRS) score (≤ 2 as good prognosis, > 2 as poor, = 6 as death) at discharge were obtained to compare the differences between the two groups. Logistic regression was used to analyze the factors influencing on the outcome. Results: Among a total of 1663 AIS patients, there were 280 had AF, of them 227 AF-AIS cases were conformed to the inclusion criteria, including 45 in group A and 182 in group B. All of AF-AIS patients, 48.0% had larger size of infarction and 62.1% had National Institute of Health stroke scale (NIHSS)score more than 10, the differences in the size and NIHSS between the two groups were not significant. A total of 51 cases (22.5%) died during hospitalization, the difference between group A and group B was not obvious (20.0% vs. 23.1%, P=0.658). The cumulative poor outcome (including deaths) at discharge was 75.3%, the difference between the two groups was also not significant (77.8% vs. 74.7%, P=0.671). The incidence of hemorrhagic transformation (HT) in group A was higher than that of in group B (40.0% vs. 21.4, P=0.010), the same was true for parenchymal hematoma (PH) in group A than group B (22.2% vs. 5.5%, P = 0.001). On univariate analysis, poor outcome was significantly associated with infarct size, NIHSS and PH, but not thrombolysis. The proportion of PH in patients with poor outcome between the two groups was also not remarkable. On adjusted multiple logistic regression analysis, both baseline infarction size [(P=0.013, odds ratio (OR) =4.558, 95% 95% confidence interval (CI): 1.373- 15.133] and NIHSS (P<0.001, OR=1.348, 95% CI=1.219-1.491) but not thrombolysis or PH entered into the final model as significant independent risk factors of poor outcome. Conclusion: Patients with AF-AIS had larger infarction size, higher NIHSS score, higher rate of mortality and worse outcome, for them, the IV rt-PA increased the incidence of PH except significantly improved their short-term prognosis.
In silico studies on milk derived peptides as potential inhibitors against SARS CoV-2 spike protein receptor binding domain
Authors: Mukhopadhyay Sutanu , Sarkar Anasua 
Published: June 2021

Abstract
COVID-19 (Corona Virus Infected Diseases-19) is caused by a strain of coronavirus called SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2). There’s no permanent diagnosis available till date to combat the disease. The viral infection in humans is initiated by binding of RBD (receptor binding domain) of spike protein to human angiotensin-converting enzyme 2 (hACE2) receptor protein. In this computational study, milk-derived peptides are screened against Receptor Binding Domain (RBD) of spike protein of the virus. Milk is considered as one of the most nutrient-rich liquid foods having several antibacterial and antiviral activities. Milk derived peptides including Casein and Whey derived peptides are known to have profound anti-viral and immunomodulatory activities. After extensive literature search, some peptides having anti-viral activities against different viruses, are shortlisted for this study and their three-dimensional structures are modelled for protein-peptide docking against SARS-CoV-2 spike protein RBD. After performing protein-peptide docking and protein-protein docking using different servers such as HPEPDOCK, FIREDOCK, HADDOCK, HDOCK, it has been observed that in presence of the peptides, the interaction between spike RBD and hACE2 has been reasonably decreased, which implies that milk-derived peptides can be potential peptide-inhibitors against the RBD of the virus along with other medications. Further studies on milk-derived peptides should be performed to develop peptide drugs based on milk-derived peptides.
Evolution of the Efficiency of Divalent Cobalt and Copper Chelates Based on Isatin Derivatives and Thiosemicarbazide Ligands as Inhibitors for the Corrosion of Sabic Iron in Acidic Medium
Authors: El-Ghamry Hoda A. , Fawzy Ahmed , Farghaly Thoraya A. , Bawazeer Tahani M. , Alqarni Nada , Alkhatib Fatmah M. , Gaber Mohamed 
Published: June 2021

Abstract
Divalent cobalt and copper chelates of the two ligands 1-(1- Ethoxycarbonylmethyl-2-oxoindolin-3-ylidene) thiosemicarbazide (EOIT) and 1-(1-Benzyl-2-oxoindolin-3-ylidene) thiosemicarbazide (BOIT) are the target compounds of the current study. Identification of the constitution and geometry of these compounds have been performed using the possible physicochemical and analytical instruments. Elemental analysis, molar conductance and thermal analysis assured the composition of the four chelates to be [Co(POIT)Cl]•1.5H2O, [Cu(POIT)Cl], [Co(BOIT)Cl2(H2O)]•5H2O and [Cu(BOIT)Cl]•Cl which was further confirmed by the measurement of mass spectra. The architecture arrangement of the ligand atoms around Co and Cu centers has been determined depending on the UV-Vis spectral measurements and calculation of µeff values assuring the copper compounds to be square plane whereas the cobalt complexes have tetrahedral or octahedral arrangements. These compounds were examined as corrosion inhibitors for the Sabic steel in 1.0 M HCl utilizing potentiodynamic polarization, electrochemical impedance spectroscopy, mass-loss method and scanning electron microscopy at fixed temperature of 298 K. The acquired outcomes disclosed that the inhibition efficiencies (% IEs) of the examined compounds were set to be dependent on both the concentrations and composition of the compounds. The examined compounds were set to have high % IEs, which were interpreted by strong adsorption of the compounds’ molecules on the iron surface and such adsorption was discovered to follow Langmuir adsorption isotherm. The investigational results obtained from all utilized techniques were set to be in a good accord with each other.
Diagnosis, prognosis and clinical treatment of refractory atypical chronic lymphocytic leukemia with multiple B cells: A case report.
Authors: Chen Man , Sun Huipeng , Zhang Lina , Gao Haiyan , Fu Minjing , Li Ting , Wang Aixian , Wu Xueying , Zhen Junyi , Gong Meiwei , Du Qing , Wu Ping , Wang Tong , Liu Hongxing , Zhou Xiaoge , Wang Hui 
Published: June 2021

Abstract
Background: Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) is the most prevalent adult leukemia, and its incidence continues to rise year after year. Rapid and precise diagnosis is an essential element in effective case management, however, the clinical diagnosis, treatment, and prognosis of CLL/SLL are not fully elucidated. Case presentation: we report the case of a 66-year-old man with atypical CLL/SLL. The white blood cell (WBC) count (842.0 × 109/L), platelet count (30.6 × 109/L), and abnormal lymphocytes were increased in peripheral blood. Flow cytometry showed 98.34% of nucleated cells were malignant monoclonal mature B cell. Peripheral blood smear found the leukocytes and lymphocytes with abnormal morphology were increased. Fluorescence in situ hybridization showed CCND1 (11q23)/IGH (14q32) and abnormal chromosome 12 were invisible, 91%-93% of interphase nuclei presented D13S319 and TP53, 17p13.1 loss. Histopathology analysis of bone marrow observed the proliferation centers with immunoblasts. Immunohistochemistry showed that bone marrow was positive for PAX-5, CD20, CD23, and CD5, negative for CD3, cyclinD1, and sox11, and partial positive for Ki67. The patient was diagnosed as CLL/SLL based on above clinical and laboratory findings. The patient was managed with oral 50 mg Vinetoc, fluid replacement, hydration and alkalinization, and the symptoms were significantly relieved. Conclusions: This report further expands the knowledge of clinical diagnosis and treatment of atypical CLL/SLL.
Housing situation, residential Relationship and the prevention of chronic diseases of the elderly
Authors: Yajun Duan , Juan Luo 
Published: June 2021

Abstract
Background:To understand the impact of housing and living-related factors on the chronic diseases of the elderly floating population, and to provide preventive measures for reducing the risk of chronic diseases among the elderly floating population in my country. Methods:In this paper, we used the data from the 2017 Migrant Population Dynamic Monitoring Survey in China, selected housing and housing-related factors as explanatory variables, and adopted a binary logistic regression analysis method. Result:People living in free housing are more mobile and more susceptible to chronic diseases; most people in self-owned housing need to repay their mortgages in their daily lives, and there is a certain life pressure that affects them to enjoy a high-quality life; elderly floating population who migrate with spouses or children Life pressure is heavier, and there is a greater probability of chronic disease risk. Conclusion:This article proposes that all localities should strictly control housing prices, increase housing supply for the floating population, improve the service level of the elderly floating population, improve the social security system and mechanisms, and effectively improve the happiness of the elderly floating population.
Fluorescence Method in Measuring the Degree of Brine Penetration into Boneless Whole-Muscle Turkey Meat
Authors: Moiseeva Natalia , Chekryga Galina , Motovilov Oleg , Golub Olga 
Published: June 2021

Introduction. Fluorescence is one of the most promising methods of food quality tests. Fluorescein disodium salt is used as a means of diagnostics in medicine and food production. In particular, fluorophore can determine the ripening stage of boneless turkey meat during brining by defining the penetration depth of the brine.
Study objects and methods. The present research featured samples of brined turkey breast and thigh meat. The experiment was based on standard research methods, e.g. macro- and microscopy, statistical method, etc. It also included an original fluorescence method for determining the penetration depth of sodium chloride brine into turkey muscles.
Results and discussion. The experimental brine consisted of food salt, white sugar, water, and fluorescein disodium salt, while brine without fluorophore served as control. Regardless of sodium chloride concentration, the changes in the ripening depth of the turkey breast samples were 8 times lower after 5 min, 3 times – after 12 h, and 4 times after 24 h. Complete ripening occurred after 36 h, which was confirmed by visual and fluorophore measurements. The thigh meat samples showed a smaller area of sodium chloride penetration: 8 times lower – after 5 min, 2 times – after 12 and 24 h. Complete salting was also registered after 36 h of brining.
Conclusion. The fluorescence method proved quite effective in determining the ripening of boneless turkey parts meat.
Forming Soy Malt Quality with Organic Growth Promoters
Authors: Miller Yuliya , Kiseleva Tatyana , Arysheva Iulia 
Published: June 2021

Introduction. Soy is one of the most promising plant raw materials as it is rich in proteins and amino acids. However, its content of anti-nutritive compounds is too high to be used in food and beverage industry without precure. The research objective was to obtain soy-based malt with a high enzymatic activity, low content of anti-nutritional substances, and increased nutritional value.
Study objects and methods. The study featured Far-Eastern soybean varieties and malt. It involved standard methods of quality control of raw materials, semi-finished products, and finished products of beer and alcohol industry, as well as the capillary electrophoresis, spectrophotometry, and potentiometry.
Results and discussion. Germination of soybeans of all varieties contributed to the accumulation of hydrolytic enzymes and amino acids in the grain. The use of a complex of organic acids from the Krebs cycle at a concentration of 10–9 mol/dm3 at the soaking stage increased amylolytic, proteolytic, and lipoxygenase hydrolytic processes by 11, 22, and 12%, respectively. The level of urease, which correlates with the content of anti-nutritional substances, decreased by two times from the original level and was 0.4–0.5 units of pH. Germination stage fell down to 2.5–3 days, while the content of amino acids increased by 33–35% in comparison with unprocessed malt during soaking.
Conclusion. The use of organic acids in soy malting improved the quality and technological indicators, increased the level of amino acids, and decreased the level of anti-nutritional substances, making soy malt suitable for beverage industry.
Assessment of Functional Properties and Safety Indicators of Amaranth Flour Grain Bread
Authors: Alekhina Nadezhda , Ponomareva Elena , Zharkova Irina , Grebenshchikov Andrej 
Published: June 2021

Introduction. Various formulations of sprouted grain breads, including those with amaranth flour, were developed to combat food-related diseases. Healthy food industry requires thorough assessment procedures and hygienic practices. The research objective was to assess the functional properties and safety indicators of amaranth bread made from sprouted wheat grain.
Study objects and methods. One day old grain bread was tested for antioxidant activity using amperometric method. Its glycemic index was determined according to the ratio of the area under the glycemic curve per bread sample to the area under the glycemic curve for pure glucose, expressed as a percentage. Crumb proteins digestibility was measured in vivo using ciliates Paramecium caudatum. Phytin content was measured by the colorimetric method, while safety indicators were compared to the Technical Regulations of the Customs Union No. 021/2011. Bound moisture content was determined with a refractometer after three days of storage according to the change in sucrose concentration. The number of mesophilic aerobic and facultative anaerobic microorganisms (NMAFAnM) was described based on State Standard 10444.15-94.
Results and discussion. The amaranth flour slightly affected the antioxidant activity of the grain bread. It decreased the glycemic index by 8.3% and increased the microbiological purity by 1.4 times, raised the crumb proteins digestibility by 3.0%, reduced the phytin content by 7.0%, and slowed down the staleness process by 12 h. In terms of safety indicators, the obtained sample of amaranth grain bread met the TR CU 021/2011 requirements “On food safety”. The decrease in the glycemic index values could be explained by the lower activity of amylolytic enzymes in amaranth flour compared to sprouted wheat. The low digestibility of carbohydrates resulted from their effect on starch. The increase in digestibility and the decrease in phytin content were caused by the lower amount of dietary fiber. The high content of bound moisture explains the longer shelf life. Amaranth flour had lower microbiological contamination compared to wheat, which decreased the NMAFAnM.
Conclusion. Amaranth flour had a positive effect on the functional properties of grain bread safety indicators, which makes it possible to include it into sprouted wheat grain products.
An Overview of Zinc Oxide Nanoparticles Produced by Plant Extracts
for Anti-tuberculosis Treatments
Authors: Behzad Farahnaz , Sefidgar Erfan , Samadi Azam , Lin Wensen , Pouladi Iman , Pi Jiang 
Published: June 2021

:
Tuberculosis (TB), induced by Mycobacterium tuberculosis (MTB), is a fatal infectious disease
that kills millions of lives worldwide. The emergence of drug-resistant and multidrug-resistant
cases is regarded as one of the most challenging threats to TB control due to the low cure rate. Therefore,
TB and drug-resistant TB epidemic urge us to explore more effective therapies. The increasing
knowledge of nanotechnology has extended the use of some nanomedicines for disease treatment in
clinics, which also provide novel possibilities for nano-based medicines for TB treatment. Zinc oxide
nanoparticles (ZnO NPs) have gained increasing attention for anti-bacterial uses based on their strong
ability to induce reactive oxidative species (ROS) and release bactericidal Zinc ions (Zn2+), which are
expected to act as novel strategies for TB and drug-resistant TB treatment. Some plant extracts, always
from active herbal medicines, have been widely reported to show attractive anti-bacterial activity for
infectious treatment, including TB. Here, we summarize the synthesis of ZnO NPs using plant extracts
(green synthesized ZnO NPs), and further discuss their potentials for anti-TB treatments. This is the
first review article discussing the anti-TB activity of ZnO NPs produced using plant extracts, which
might contribute to the further applications of green synthesized ZnO NPs for anti-TB and drugresistant
TB treatment.
ALK7 Inhibition Protects Osteoblast Cells Against High Glucoseinduced
ROS Production via Nrf2/HO-1 Signaling Pathway
Authors: Zhao Zhen , Lu Yu , Wang Huan , Gu Xiang , Zhu Luting , Guo Hong , Li Nan 
Published: June 2021

Background:
Some studies demonstrated that under high-glucose (HG)
condition, osteoblasts develop oxidative stress, which will impair their normal functions.
The effects of activin receptor-like kinase 7 (ALK7) silencing on HG-induced osteoblasts
remained unclear.

Objective:
The aim of this study was to explore the effect of ALK7 on HG-induced
osteoblasts.

Methods:
MC3T3-E1 cells were treated with different concentrations of HG (0, 50, 100,
200 and 300mg/dL), and the cell viability was detected using cell counting kit-8 (CCK-8).
HG-treated MC3T3-E1 cells were transfected with siALK7 or ALK7 overexpression
plasmid or siNrf2, and then the viability and apoptosis were detected by CCK-8 and flow
cytometry. The levels of Reactive Oxygen Species (ROS), collagen I and calcification
nodule were determined by oxidative stress kits, Enzyme-linked immunosorbent assay
and Alizarin red staining. The expressions of NF-E2-related factor 2 (Nrf2), heme
oxygenase-1 (HO-1) and osteoblast-associated genes were determined by quantitative
real-time PCR (qRT-PCR) and Western blot.

Results:
Cell viability was reduced with HG treatment. Silencing ALK7 inhibited the
effect of HG on increasing cell apoptosis and ROS production, reduced cell viability,
mineralized nodules, and downregulated collagen I and osteoblast-associated genes
expression in MC3T3-E1 cells. ALK7 silencing activated the Nrf2/HO-1 signaling
pathway by affecting expressions of HO-1 and Nrf2. ALK7 overexpression had the
opposite effects. In addition, siNrf2 partially reversed the effects of ALK7 silencing on
HG-induced MC3T3-E1 cells.

Conclusion:
ALK7 silencing protected osteoblasts under HG condition possibly through
activating the Nrf2/HO-1 pathway.
Gastroretentive Metformin Loaded Nanoparticles for the Effective Management
of Type-2 Diabetes Mellitus
Authors: Jain Akhlesh K. , Upadhyay Richa , Mishra Keerti , Jain Sunil K. 
Published: June 2021

Introduction:
Metformin, an anti-diabetic drug, has low bioavailability and short biological
half-life. Thus, bioavailability enhancement and prolonged release of the drug are highly desirable.
In this regard, we aimed to developed gastroretentive nanoparticles made of jackfruit seed
starch (JFSS) loaded with metformin.

Methods:
Developed nanoparticles were optimized for various process variables and were further
characterized. Nanoparticles exhibited good results with respect to particle size (244.3 to 612.4
nm), particle size distribution, shape and drug entrapment efficiency (75.8 to 89.2%) with sustained
drug release for 24 h and a high buoyancy (89% for F7; formulation made of highest concentration
of Jackfruit seed starch prepared at 1000 RPM stirring speed).

Results:
The hypoglycemic potential of these nanoparticles was tested in nicotinamide streptozocin
induced diabetic model, there was a significant reduction in blood glucose level (50% reduction
from 4-8 h; p < 0.01) for prolonged period of time (up to 24 h) in comparison to diabetic control
and plain metformin solution.

Conclusion:
The outcome of the study suggested that developed formulations are suitable for gastro-
retentive delivery of Metformin in a controlled manner appropriate for a single administration
per day.
Assessment of Exenatide loaded Biotinylated Trimethylated Chitosan/HP-
55 Nanoparticles
Authors: Guo Hejian , Yan Xuehui , Tang Hao , Zhang Xiaoyan 
Published: June 2021

Background:
Exenatide(EXE) is an anti-hyperglycemic agent approved for treating type
2 diabetes by the Food and Drug Administration(FDA). However, twice-daily injection of exenatide
is inconvenient for most of the patients.

Objective:
In this study, biotinylated trimethylated chitosan(Bio-TMC) based nanoparticles were
proposed to promote oral absorption of exenatide. Realizing the oral administration of exenatide is
very important to alleviate patient suffering and improve patient compliance.

Methods:
Bio-TMC was synthesized, and the chemical structure was characterized by Fourier
transform infrared (FT-IR) spectroscopy and 1H NMR spectroscopy. Nanoparticles were prepared
through polyelectrolyte interaction in the presence of sodium Tripolyphosphate (TPP) and hydroxypropyl
methylcellulose phthalate (HP-55). Formulations were physically and chemically characterized.
In vitro release was investigated in different pH media. In vivo antidiabetic activities of biotin
modified and non-biotin modified chitosan were evaluated in db/db mice.

Results:
EXE-loaded Bio-TMC/HP-55 nanoparticles were spherical in shape with a mean diameter
of 156.2 nm and zeta potential of +11.3 mV. The drug loading efficiency and loading content were
52.38% and 2.08%, respectively. In vitro release revealed that EXE-loaded Bio-TMC/HP-55 nanoparticles
were released faster in pH 1.2 than pH 6.8 (63.71% VS 50.12%), indicating that nanoparticles
have enteric characteristics. Antidiabetic activity study revealed that after oral administration
to diabetic mice, the relative pharmacological bioavailability (FPharm%) of the biotin modified nanoparticles
was found to be 1.27-fold higher compared to the unmodified ones, and the hypoglycemic
effect was also found to be better.

Conclusion:
Bio-TMC/HP-55 nanoparticles are feasible as oral drug carriers of exenatide and have
the potential to be extended to other drugs that are not readily oral, such as monoclonal antibodies,
vaccines, genes, etc. These would be beneficial to the pharmaceutical industry. Further research
will focus on the biodistribution of Bio-TMC/HP-55 nanoparticles after oral administration.