bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determining the impact of the Zika pandemic on primary care providers’ contraceptive counseling of non-pregnant patients in the US: a mixed methods study
Authors: Kaiser Jennifer E. , Galindo Eduardo , Sanders Jessica N. , Simmons Rebecca G. , Gawron Lori M. , Herrick Jennifer S. , Brintz Benjamin , Turok David K. 
Published: November 2021

Abstract
Background
Global pandemics like Zika (ZIKV) factor into pregnancy planning and avoidance, yet little is known about how primary care providers (PCPs) incorporate public health guidance into contraceptive counseling. Study objectives include: 1) determining the impact of the ZIKV pandemic on contraceptive counseling changes; and 2) assessing PCP knowledge and practice regarding contraception, ZIKV, and CDC ZIKV guidelines.

Methods
Study components included: (1) a retrospective review of electronic health records of non-pregnant, reproductive age women presenting for preventive health visits between 2014 and 2017 assessed using interrupted time series analyses (ITSA) to identify changes in documentation of ZIKV risk assessment and contraceptive counseling; and (2) a sequential, cross-sectional study with quantitative surveys and qualitative, semi-structured interviews of PCPs providing preventive care to non-pregnant patients at eight federally qualified health centers in Utah. We performed descriptive analyses on survey data and analyzed qualitative data for dominant themes using a modified Health Belief Model.

Results
We conducted 6634 chart reviews yielding 9840 visits. The ITSA did not reveal changes in ZIKV risk assessment or contraceptive counseling. Twenty-two out of 40 (55%) eligible providers participated in the provider component. Participants averaged 69 and 81% correct on contraceptive and ZIKV knowledge questions, respectively. Sixty-five percent reported counseling consistent with CDC ZIKV guidelines. Qualitative analysis found providers unlikely to prioritize ZIKV risk assessment in contraceptive counseling for non-pregnant patients.

Conclusions
PCPs who care for non-pregnant women are knowledgeable about contraception and ZIKV; however, there was no change in ZIKV risk assessment or contraceptive counseling. This stresses the importance of developing strategies to improve guideline uptake.
A parsimonious model to validate cost-effectiveness analyses on preventive health care
Authors: Gandjour Afschin 
Published: November 2021

Abstract
Background
The effect of preventive health care on health expenditures is ambiguous. On the one hand, prevention reduces the costs of future morbidity. On the other hand, prevention leads to costs of life extension. The purpose of this paper is to develop a parsimonious model that determines for a preventive measure of interest whether savings from preventing morbidity are more than offset by the costs of living longer, resulting in a net expenditure increase.

Methods
A theoretical model was built based on a Weibull survival function. It includes savings and life extension costs over the remaining lifetime. The model was applied to the example of obesity prevention.

Results
The model shows that the cost consequences of prevention are essentially driven by two factors: i) the relative reduction of morbidity-related costs, which determines the amount of savings from avoiding morbidity; and ii) the hazard ratio of death, which determines the amount of life extension costs. In the application example, the model is able to validate the results of a more complex cost-effectiveness model on obesity prevention.

Conclusions
This work provides new insight into the lifetime cost consequences of prevention. The model can be used both to check plausibility of the results of other models and to conduct an independent analysis.
Consideration factors of older adults seeking medical treatment at outpatient services in Taiwan
Authors: Shih Chen-I , Weng Cheng-Chie , Chen Wei , Yang Hui-Fei , Fan Sheng-Yu 
Published: November 2021

Abstract
Background
Taiwan will become a super-aged society by 2025, leading to the more frequent use of outpatient services by older adults for medical treatment compared with other age groups. Understanding the outpatient service consideration factors of older adults seeking medical treatment can improve health care quality. This study explored the selection factors and crucial considerations of older adults for outpatient services.

Methods
Qualitative study was conducted. Purposive sampling was used to recruit 16 older adults over 65 years of age with chronic disease who were patients of an internal medicine department and regularly returned for checkups. Data including reasons for receiving medical treatment, factors affecting their choice of hospitals, and health care and environmental considerations were collected through structured interviews.

Results
The older adults identified four factors. (1) The care of doctors: The doctors possessed professional skill, allocate sufficient consultation time, and undertake effective communication. (2) The care of other medical professionals: Other medical professionals provided services in a cordial manner. (3) The accessibility and convenience of outpatient services: Convenient transportation and registration as well as short consultation wait time. (4) Environment and equipment: The hospital had the novel facilities and satisfactory barrier-free equipment.

Conclusions
The older adults cared most about the adequacy of diagnosis and treatment by doctors and other medical professionals. In addition, they reported having higher satisfaction with hospitals that provide comprehensive medical facilities, fast and convenient medical procedures, and short wait times.
The interactions between dietary fats intake and Caveolin 1 rs 3807992 polymorphism with fat distribution in overweight and obese women: a cross-sectional study
Authors: Aali Yasaman , Shiraseb Farideh , Abaj Faezeh , koohdani Fariba , Mirzaei Khadijeh 
Published: November 2021

AbstractBackgroundIt has been reported that dietary fats and genetic factors in individuals are associated with the pattern of fat distribution. This study aimed to evaluate the interaction between dietary fats intake andCaveolin1 (CAV-1) rs 3807s992polymorphism with fat distribution in overweight and obese women.MethodsA total of 221 participants were included in the current cross-sectional study. Body composition, biochemical parameters were evaluated by body composition analyzer and Pars Azmoon kits and genotypes determination was performed by PCR–RFLP, dietary fats were measured using a validated semi-quantitative food frequency questionnaire (FAQ).ResultsThe frequency of GG, AA and AG genotypes were 53.1, 24.6, and 22.3%, respectively, and the mean intake of total dietary fat intake was 97.47 ± 36.87 g. There was positive significant interaction between total fat intake and AA genotype on visceral fat level (p = 0.001), trunk fat (p = 0.01) and waist circumference (p = 0.05), positive significant interaction between total fat intake and AG genotype on the waist to hip ratio (WHR) (p = 0.02) and visceral fat level (p = 0.05), positive borderline significant interaction between saturated fatty acid and AA genotype on the trunk fat (p = 0.06), and between trans-fatty acids and AG genotype on WHR (p = 0.04), visceral fat level (p = 0.01), and between monounsaturated fatty acid and AG genotype on WHR (p = 0.04), and a borderline interaction between polyunsaturated fatty acid and AA genotypes on visceral fat level (p = 0.06), negative significant interaction between AG genotypes and linolenic acid on WHR (p = 0.04), borderline significant interaction between ALA and AG genotype on WHR (p = 0.06).ConclusionsOur findings showed thatCAV-1 rs 3807992polymorphism and dietary fats were associated with fat distributions in individuals.
Genetic analysis of osteopetrosis in Pakistani families identifies novel and known sequence variants
Authors: Liu Chunyu , Ajmal Muhammad , Akram Zaineb , Ghafoor Tariq , Farhan Muhammad , Shafique Sobia , Wahid Sughra , Bano Shahar , Xiao Jianqiu , Satti Humayoon Shafique , Zhang Feng , Khan Tahir Naeem 
Published: November 2021

AbstractOsteopetrosis is a genetically heterogenous, fatal bone disorder characterized by increased bone density. Globally, various genetic causes are reported for osteopetrosis with all forms of inheritance patterns. A precise molecular diagnosis is necessary for prognosis and for prescribing treatment paradigms in osteopetrosis. Here we report on thirteen individuals diagnosed with infantile malignant osteopetrosis coming from ten unrelated Pakistani families; nine of whom are consanguineous. We performed whole exome sequencing and Sanger sequencing in all families and identified homozygous variants in genes previously reported for autosomal recessive inheritance of osteopetrosis. All the identified variants are expected to affect the stability or length of gene products except one nonsynonymous missense variant. TCIRG1 was found as a candidate causal gene in majority of the families. We report six novel variants; four in TCIRG1 and one each in CLCN7 and OSTM1. Our combined findings will be helpful in molecular diagnosis and genetic counselling of patients with osteopetrosis particularly in populations with high consanguinity.
Low-grade inflammation as a risk factor for cardiovascular events and all-cause mortality in patients with type 2 diabetes
Authors: Sharif Shahnam , Van der Graaf Y. , Cramer M. J. , Kapelle L. J. , de Borst G. J. , Visseren Frank L. J. , Westerink Jan ,   the SMART study group, van Petersen R. , Dinther B. G. F. , Algra A. , van der Graaf Y. , Grobbee D. E. , Rutten G. E. H. M. , Visseren F. L. J. , de Borst G. J. , Kappelle L. J. , Leiner T. , Nathoe H. M. 
Published: November 2021

Abstract
Background
Type 2 diabetes is a condition associated with a state of low-grade inflammation caused by adipose tissue dysfunction and insulin resistance. High sensitive-CRP (hs-CRP) is a marker for systemic low-grade inflammation and higher plasma levels have been associated with cardiovascular events in various populations. The aim of the current study is to evaluate the relation between hs-CRP and incident cardiovascular events and all-cause mortality in high-risk type 2 diabetes patients.

Methods
Prospective cohort study of 1679 type 2 diabetes patients included in the Second Manifestations of ARTerial disease (SMART). Cox proportional hazard models were used to evaluate the risk of hs-CRP on cardiovascular events (composite of myocardial infarction, stroke and vascular mortality) and all-cause mortality. Hs-CRP was log-transformed for continuous analyses. Findings were adjusted for age, sex, BMI, current smoking and alcohol use, non-HDL-cholesterol and micro-albuminuria.

Results
307 new cardiovascular events and 343 deaths occurred during a median follow-up of 7.8 years (IQR 4.2–11.1). A one unit increase in log(hs-CRP) was related to an increased vascular- and all-cause mortality risk (HR 1.21, 95% CI 1.01–1.46 and HR 1.26, 95% CI 1.10–1.45 respectively). No relation was found between log(hs-CRP) and myocardial infarction or stroke. The relations were similar in patients with and without previous vascular disease.

Conclusion
Low grade inflammation, as measured by hs-CRP, is an independent risk factor for vascular- and all-cause mortality but not for cardiovascular events in high-risk type 2 diabetes patients. Chronic low-grade inflammation may be a treatment target to lower residual cardiovascular risk in type 2 diabetes patients.
Icariin improves testicular dysfunction via enhancing proliferation and inhibiting mitochondria-dependent apoptosis pathway in high-fat diet and streptozotocin-induced diabetic rats
Authors: He Weiguo , Liu Huiqing , Hu Linlin , Wang Yaohui , Huang Lane , Liang Aihong , Wang Xuan , Zhang Qing , Chen Yi , Cao Yi , Li Suyun , Wang Junli , Lei Xiaocan 
Published: November 2021

Abstract
Background
Diabetes mellitus (DM), a chronic metabolic disease, severely impairs male reproductive function. However, the underpinning mechanisms are still incompletely defined, and there are no effective strategies or medicines for these reproductive lesions. Icariin (ICA), the main active component extracted from Herba epimedii, is a flavonoid traditionally used to treat testicular dysfunction. Whether ICA can improve male reproductive dysfunction caused by DM and its underlying mechanisms are still unclear. In this study, by employing metformin as a comparative group, we evaluated the protective effects of ICA on male reproductive damages caused by DM and explored the possible mechanisms.

Methods
Rats were fed with a high fat diet (HFD) and then intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Diabetic rats were randomly divided into T2DM + saline group, T2DM + metformin group and T2DM + ICA group. Rats without the treatment of HFD and STZ were used as control group. The morphology of testicular tissues was examined by histological staining. The mRNA expression levels were determined by quantitative real-time PCR. Immunostaining detected the protein levels of proliferating cell nuclear antigen (PCNA), hypoxia-inducible factor 1-alpha (HIF-1α) and sirtuin 1 (SIRT1) in testicular tissues. TUNEL assay was performed to determine cell apoptosis in the testicular tissues. The protein expression levels of HIF-1α and SIRT1 in the testicular tissues were determined by western blot assay.

Results
ICA effectively improved male reproductive dysfunction of diabetic rats. ICA administration significantly decreased fasting blood glucose (FBG) and insulin resistance index (IRI). In addition, ICA increased testis weight, epididymis weight, sperm number, sperm motility and the cross-sectional area of seminiferous tubule. ICA recovered the number of spermatogonia, primary spermatocytes and Sertoli cells. Furthermore, ICA upregulated the expression of PCNA, activated SRIT1-HIF-1α signaling pathway, and inhibited intrinsic mitochondria dependent apoptosis pathway by upregulating the expression of Bcl-2 and downregulating the expression of Bax and caspase 3.

Conclusion
These results suggest that ICA could attenuate male reproductive dysfunction of diabetic rats possibly via increasing cell proliferation and decreasing cell apoptosis of testis. ICA potentially represents a novel therapeutic strategy against DM-induced testicular damages.
Therapeutic inhibition of GAS6-AS1/YBX1/MYC axis suppresses cell propagation and disease progression of acute myeloid leukemia
Authors: Zhou Hao , Liu Wei , Zhou Yongming , Hong Zhenya , Ni Jian , Zhang Xiaoping , Li Ziping , Li Mengyuan , He Wenjuan , Zhang Donghua , Chen Xuexing , Zhu Jianhua 
Published: November 2021

Abstract
Background
Acute myeloid leukemia (AML) is the most common type of leukemia in adults. Its therapy has not significantly improved during the past four decades despite intense research efforts. New molecularly targeted therapies are in great need. The proto-oncogene c-Myc (MYC) is an attractive target due to its transactivation role in multiple signaling cascades. Deregulation of the MYC is considered one of a series of oncogenic events required for tumorigenesis. However, limited knowledge is available on which mechanism underlie MYC dysregulation and how long non-coding RNAs (lncRNAs) are involved in MYC dysregulation in AML.

Methods
AML microarray chips and public datasets were screened to identify novel lncRNA GAS6-AS1 was dysregulated in AML. Gain or loss of functional leukemia cell models were produced, and in vitro and in vivo experiments were applied to demonstrate its leukemogenic phenotypes. Interactive network analyses were performed to define intrinsic mechanism.

Results
We identified GAS6-AS1 was overexpressed in AML, and its aberrant function lead to more aggressive leukemia phenotypes and poorer survival outcomes. We revealed that GAS6-AS1 directly binds Y-box binding protein 1 (YBX1) to facilitate its interaction with MYC, leading to MYC transactivation and upregulation of IL1R1, RAB27B and other MYC target genes associated with leukemia progression. Further, lentiviral-based GAS6-AS1 silencing inhibited leukemia progression in vivo.

Conclusions
Our findings revealed a previously unappreciated role of GAS6-AS1 as an oncogenic lncRNA in AML progression and prognostic prediction. Importantly, we demonstrated that therapeutic targeting of the GAS6-AS1/YBX1/MYC axis inhibits AML cellular propagation and disease progression. Our insight in lncRNA associated MYC-driven leukemogenesis may contribute to develop new anti-leukemia treatment strategies.
The Mie representation for Mercury’s magnetospheric currents
Authors: Toepfer S. , Narita Y. , Exner W. , Heyner D. , Kolhey P. , Glassmeier K. -H. , Motschmann U. 
Published: November 2021

AbstractPoloidal–toroidal magnetic field decomposition is a useful application of the Mie representation and the decomposition method enables us to determine the current density observationally and unambiguously in the local region of magnetic field measurement. The application and the limits of the decomposition method are tested against the Mercury magnetic field simulation in view of BepiColombo’s arrival at Mercury in 2025. The simulated magnetic field data are evaluated along the planned Mercury Planetary Orbiter (MPO) trajectories and the current system that is crossed by the spacecraft is extracted from the magnetic field measurements. Afterwards, the resulting currents are classified in terms of the established current system in the vicinity of Mercury.
Graphical Abstract
Efficacy of Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales) against Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) under controlled and open-field conditions on bitter gourd
Authors: Hamzah Abdul Mannan , Mohsin Ata ul , Naeem Muhammad , Khan Muhammad Azam 
Published: November 2021

AbstractBackgroundBactrocera cucurbitae(Coquillett) (Diptera: Tephritidae) is the most destructive pest of bitter gourd. Conventionally, it is controlled by chemical insecticides despite their hazardous impacts. Entomopathogenic fungi are considered as eco-friendly and safer alternative of chemical. This study aimed to evaluate the efficacy ofBeauveria bassianaandMetarhizium anisopliaeagainstB. cucurbitaeon bitter gourd.ResultsThe study revealed thatB. bassianaandM. anisopliaewere efficient againstB. cucurbitaein controlled as well as field conditions. Both mode of application (i.e., contact and oral) were found to be effective; however, high efficacies of them were observed through contact application (73.43 and 59.72%), respectively. Pathogenicity of both fungi increased with increase in concentration and time intervals. Under field conditions, significantly low fruit infestations and significantly a high population reduction ofB. cucurbitaewere observed at 30 DAT when both fungi were applied with 108 CFU/ml concentrations.ConclusionsBeauveria bassianaandM. anisopliaehad the potential to be used againstB. cucurbitae;however, the former showed high efficiency comparatively.
Improvised centrifugal spinning for the production of polystyrene microfibers from waste expanded polystyrene foam and its potential application for oil adsorption
Authors: Budlayan Marco Laurence M. , Patricio Jonathan N. , Lagare-Oracion Jeanne Phyre , Arco Susan D. , Alguno Arnold C. , Basilio Antonio , Latayada Felmer S. , Capangpangan Rey Y. 
Published: November 2021

AbstractA straightforward approach to recycle waste expanded polystyrene (EPS) foam to produce polystyrene (PS) microfibers using the improvised centrifugal spinning technique is demonstrated in this work. A typical benchtop centrifuge was improvised and used as a centrifugal spinning device. The obtained PS microfibers were characterized for their potential application for oil adsorption. Fourier transform infrared spectroscopy results revealed similarity on the transmission bands of EPS foam and PS microfibers suggesting the preservation of the EPS foam’s chemical composition after the centrifugal spinning process. Scanning electron microscopy displayed well-defined fibers with an average diameter of 3.14 ± 0.59 μm. At the same time, energy dispersive X-ray spectroscopy revealed the presence of carbon and oxygen as the primary components of the fibers. Contact angle (θCA) measurements showed the more enhanced hydrophobicity of the PS microfiber (θCA= 100.2 ± 1.3°) compared to the untreated EPS foam (θCA= 92.9 ± 3.5°). The PS microfiber also displayed better oleophilicity compared to EPS foam. Finally, the fabricated PS microfibers demonstrated promising potential for oil removal in water with a calculated sorption capacity value of about 15.5 g/g even at a very short contact time. The fabricated PS fiber from the waste EPS foam may provide valuable insights into the valorization of polymeric waste materials for environmental and other related applications.
A survey on advances of quantum repeater
Authors: Yan Pei-Shun , Zhou Lan , Zhong Wei , Sheng Yu-Bo 
Published: November 2021

Abstract
The distribution of quantum entanglement is essential for quantum communication. Contrary to classical communication, the signal in quantum communication is impossible to copy due to the basic principles of quantum mechanics, i.e., quantum state collapse and no-cloning theorem. However, the communication range is limited by inevitable photon loss. To counteract this issue, one can insert several repeater stations between two remote end nodes to divide the full distance into several shorter segments and successively operate entanglement swapping between adjacent repeater stations to enable long-distance quantum communication. Up to now, one can classify quantum repeaters (QRs) into three generations based on the methods of reducing errors resulted from photon loss and imperfect operations. In this paper, we will briefly review advances for each generation of QRs and discuss the advantages and challenges of each generation. Moreover, we also discuss some efficient proposals of building blocks to overcome the bottlenecks for QRs.
A Longitudinal Study of Medial Temporal Lobe Volumes in Graves Disease
Authors: Holmberg Mats , Malmgren Helge , Heckemann Rolf A , Johansson Birgitta , Klasson Niklas , Olsson Erik , Skau Simon , Starck Göran , Filipsson Nyström Helena 
Published: November 2021

Abstract

Context
Neuropsychiatric symptoms are common features of Graves disease (GD) in hyperthyroidism and after treatment. The mechanism behind these symptoms is unknown, but reduced hippocampal volumes have been observed in association with increased thyroid hormone levels.

Objective
This work aimed at investigating GD influence on regional medial temporal lobe (MTL) volumes.

Methods
Sixty-two women with newly diagnosed GD underwent assessment including magnetic resonance (MR) imaging in hyperthyroidism and 48 of them were followed up after a mean of 16.4 ± 4.2 SD months of treatment. Matched thyroid-healthy controls were also assessed twice at a 15-month interval. MR images were automatically segmented using multiatlas propagation with enhanced registration. Regional medial temporal lobe (MTL) volumes for amygdalae and hippocampi were compared with clinical data and data from symptom questionnaires and neuropsychological tests.

Results
Patients had smaller MTL regions than controls at inclusion. At follow-up, all 4 MTL regions had increased volumes and only the volume of the left amygdala remained reduced compared to controls. There were significant correlations between the level of thyrotropin receptor antibodies (TRAb) and MTL volumes at inclusion and also between the longitudinal difference in the levels of free 3,5,3′-triiodothyronine and TRAb and the difference in MTL volumes. There were no significant correlations between symptoms or test scores and any of the 4 MTL volumes.

Conclusion
Dynamic alterations in the amygdalae and hippocampi in GD reflect a previously unknown level of brain involvement both in the hyperthyroid state of the condition and after treatment. The clinical significance, as well as the mechanisms behind these novel findings, warrant further study of the neurological consequences of GD.