bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОПЫТ ИССЛЕДОВАНИЯ СЕРОПРЕВАЛЕНТНОСТИ К ВИРУСУ SARS-CoV-2 НАСЕЛЕНИЯ ИРКУТСКОЙ ОБЛАСТИ В ПЕРИОД ВСПЫШКИ COVID-19
Authors: Попова Анна , Ежлова Елена , Мельникова Альбина , Балахонов Сергей , Чеснокова Маргарита , Дубровина Валентина , Лялина Людмила , Смирнов Вячеслав , Трухина Анна , Пережогин Алексей , Пятидесятникова Анна , Брюхова Дарья , Киселева Наталья , Гефан Наталья , Гаврилова Ольга , Гаврилова Татьяна , Ломоносова Валерия , Тотолян Арег 
Published: July 2021

В декабре 2019 г. появилась информация о новом заболевании, этиологическим фактором которого оказался β-коронавирус SARS-CoV-2. В Иркутской области первый больной COVID-19 выявлен 21 марта 2020 года. Это был завозной случай из Объединенных Арабских Эмиратов (ОАЭ). Период нарастания интенсивности эпидемического процесса продолжался 10 недель, после чего было зафиксировано постепенное снижение. Определение серопревалентности к COVID-19 было организовано в период с 23.06.2020 по 19.07.2020 г практически на максимальном уровне заболеваемости. Работа проводилась в рамках проекта Роспотребнадзора по оценке популяционного иммунитета к SARS-CoV-2 у населения Российской Федерации с учетом протокола, рекомендованного ВОЗ. Содержание антител к SARS-CoV-2 определяли методом иммуноферментного анализа (ИФА) с использованием набора реагентов для анализа сыворотки или плазмы крови человека на наличие специфических иммуноглобулинов класса G к нуклеокапсиду вируса SARS-CoV-2 производства ФБУН ГНЦПМиБ Роспотребнадзора (г. Оболенск) в соответствии с инструкцией по применению.
Результаты исследования показали, что коллективный иммунитет совокупного населения Иркутской области составил 5,8%. Его максимальный уровень установлен у детей 14-17 лет (13,8 %) и 1-6 лет (11,8 %). Показано, что при наличии контактов с больными COVID-19 риск инфицирования возрастает в 3,1 раза. После перенесенной COVID-19 антитела вырабатываются в 56,5 % случаев. Доля бессимптомных форм среди серопозитивных жителей Иркутской области составила 81,2 %.
Результаты оценки популяционного иммунитета к вирусу SARS-CoV-2 у населения Иркутской области свидетельствуют о том, что в период эпидемического подъема заболеваемости инфекцией COVID-19 сформировался невысокий уровень серопревалентности. После перенесенного заболевания у 43 % лиц, антитела не выявлены. Значительная доля бессимптомных форм инфекции характеризует высокую интенсивность скрыто развивающегося эпидемического процесса. Полученные результаты необходимо учитывать при организации профилактических мероприятий, включая вакцинацию, и прогнозировании заболеваемости.
ОЦЕНКА ПОПУЛЯЦИОННОГО ИММУНИТЕТА К SARS-COV-2 НА ТЕРРИТОРИИ РОСТОВСКОЙ ОБЛАСТИ
Authors: Попова Анна , Ежлова Елена , Мельникова Альбина , Носков Алексей , Ковалев Евгений , Карпущенко Гарри , Лялина Людмила , Смирнов Вячеслав , Чемисова Ольга , Тришина Алена , Березняк Елена , Воловикова Софья , Стенина Снежана , Янович Евгения , Мелоян Мисак , Асмолова Наталья , Усова Анна , Слись Сергей , Тотолян Арег 
Published: July 2021

В декабре 2019 года в городе Ухань (КНР) официально зарегистрирован первый случай новой коронавирусной инфекции (COVID-19), вызванной SARS-CoV-2. Пандемическое распространение COVID-19 оказало существенное влияние на общественное здравоохранение многих стран, и 30.01.2020 г. Всемирная организация здравоохранения объявила о введении режима чрезвычайной ситуации в области общественного здравоохранения, имеющей международное значение. Заболевания людей COVID-19 отмечены более чем в 200 странах.
В Российской Федерации к августу 2020 г. подтверждено более 850 000 случаев заболевания, Ростовская область вошла в число десяти наиболее пораженных регионов России.
Распространение болезни во многом определяется состоянием популяционного иммунитета на определенной территории. Поскольку SARS-CoV-2 является новым патогеном, многие аспекты его передачи от человека к человеку и развития инфекционного процесса недостаточно охарактеризованы. Результаты массового серологического мониторинга могут составить основу для эпидемиологического прогноза, а также для разработки стратегии специфической профилактики болезни.
Целью настоящего исследования были изучение специфического гуморального иммунного ответа и оценка уровня популяционного иммунитета к вирусу SARS-CoV-2 среди населения Ростовской области.
В исследовании приняли участие 3048 человек. Волонтеры, участвовавшие в исследовании, были распределены по семи возрастным группам. Проведенная оценка серопревалентности к SARS-CoV-2 жителей Ростовской области показала, что доля лиц с положительными результатами теста на антитела IgG к новому коронавирусу составила 16,5 %, доля серопозитивных лиц в генеральной совокупности находится в пределах от 13,9% до 19,1% (р<0,05).
Достоверных гендерных различий в уровне серопревалентности не установлено, положительный результат зарегистрирован у 16,6% обследованных женщин и 16,5% мужчин. Установлен высокий уровень гуморального иммунитета к SARS-CoV-2 на фоне низких показателей заболеваемости у лиц в возрасте от 1 до 17 лет, что может свидетельствовать о доминировании бессимптомных форм болезни среди данной возрастной группы. Наибольший уровень серопозитивности выявлен среди дошкольников (33,6%), учащихся (29,3%), служащих (17,3%), работников образования (15,3%).
Generation of mouse hepatobiliary organoids from hepatocyte progenitors and cholangiocytes isolated from healthy adult mouse liver
Authors: Tanimizu Naoki , Ichinohe Norihisa , Sasaki Yasushi , Itoh Tohru , Sudo Ryo , Yamaguchi Tomoko , Katsuda Takeshi , Ninomiya Takafumi , Tokino Takashi , Ochiya Takahiro , Miyajima Atsushi , Mitaka Toshihiro 
Published: July 2021

Abstract
Number of liver organoids have been reported, though it is not clearly shown whether the functional connection between hepatocytes and cholangiocytes is recapitulated in those organoids. Here, we report generation of a hepatobiliary tubular organoid (HBTO) using mouse hepatocyte progenitors called small hepatocytes (SHs) and cholangiocytes. SHs differentiate and form the bile canalicular network in HBTOs and secret metabolites into the canaliculi, which are then transported into the biliary structure. Hepatocytes in the organoid acquire and maintain metabolic functions including albumin secretion and cytochrome P450 activities, over the long term. We provide the step-by-step protocol for induction of HBTO including isolation of cholangiocytes and SHs and co-culture of these two types of cell to generate functional connections between hepatocytes and cholangiocytes.
The impact of visuospatial perception on distance judgment and depth perception in an Augmented Reality environment in patients after stroke: an exploratory study
Authors: Höhler Chiara , Rasamoel Nils David , Rohrbach Nina , Hansen John Paulin , Jahn Klaus , Hermsdörfer Joachim , Krewer Carmen 
Published: July 2021

Abstract
Background Augmented Reality (AR)-based interventions are applied in neurorehabilitation with increasing frequency. Depth perception is required for the intended interaction within AR environments. Until now, however, it is unclear whether patients after stroke with impaired visuospatial perception (VSP) are able to perceive depth in the AR environment.Methods Different aspects of VSP (stereovision and spatial localization / visuoconstruction) were assessed in 20 patients after stroke (mean age: 64 ± 14 years) and 20 healthy subjects (HS, mean age: 28 ± 8 years) using clinical tests. The group of HS was recruited to assess the validity of the developed AR tasks in testing stereovision. To measure perception of holographic objects, three distance judgment tasks and one three-dimensionality task were designed. The effect of impaired stereovision on performance in each AR task was analyzed. AR task performance was modeled by aspects of VSP using separate regression analyses for HS and for patients.Results In HS, stereovision had a significant effect on the performance in all AR distance judgment tasks (p = .021, p = .002, p = .046) and in the three-dimensionality task (p = .003). Individual quality of stereovision significantly predicted the accuracy in each distance judgment task and was highly related to the ability to perceive holograms as three-dimensional (p = .001). In stroke-survivors, impaired stereovision had a specific deterioration effect on only one distance judgment task (p = .042), whereas the three-dimensionality task was unaffected (p = .317). Regression analyses confirmed a lacking impact of patients’ quality of stereovision on AR task performance, while spatial localization / visuoconstruction significantly prognosticated the accuracy in distance estimation of geometric objects in two AR tasks.Conclusion Impairments in VSP reduce the ability to estimate distance and to perceive three-dimensionality in an AR environment. While stereovision is key for task performance in HS, spatial localization / visuoconstruction is predominant in patients. Since impairments in VSP are present after stroke, these findings might be crucial when AR is applied for neurorehabilitative treatment. In order to maximize the therapy outcome, the design of AR games should be adapted to patients’ impaired VSP.Trial registration The trial was not registered, as it was an observational study.
Molecular Docking used as an advanced tool to determine novel compounds on emerging infectious diseases: A systematic review
Authors: Gouthami K , Veeraraghavan V , Saratale Ganesh Dattatraya , Rahdar Abbas , Bilal Muhammad , Shah Anshuman , Rai Vandna , Murari Satish Kumar , Hu Anyi , Kalia Sanjay , Mulla Sikandar 
Published: July 2021

Abstract
Emerging infectious diseases (EIDs) are among the major problems impacted on global economics and healthful living. EIDs have long been perceive as a predominant conclusion of host-pathogen adaptation. Hence, in this analysis, influence by the appearance of major factors that impact emerging infectious diseases and explicate some extensive EIDs like HIV/AIDs, H1N1 and Coronavirus. A wealth of new trends, especially in applied science is fetching progressively for the fast molecular recognizable proof of microorganisms and the more precise checking of infectious disease movement. Atomic docking is one of the prominent computational tools, involving in construction put together medication disclosure with respect to EIDs to evaluate the limiting affinities between little particles and macromolecule that can further, used as a target of HIV/AIDs, H1N1, and coronavirus. Molecular approach of influenza neuraminidase, HIV-1 protease and Spike protein of corona virus result shows Zanamivir, Nelfinavir, Saquinavir, Erythromycin and Spiramycin are favourable interaction against the selected target proteins. These novel drug compounds approved in vivo studies with reaffirm results, it is clear that the computational methods (mainly molecular docking) are found to be an effective technique for drug discovery.
Profiling Patient-Reported Symptom Recovery from Esophagectomy for Patients with Esophageal Squamous Cell Carcinoma: A Real World Longitudinal Study
Authors: Luo Xi , Xie Qin , Shi Qiuling , Miao Yan , Yu Qingsong , Yu Hongfan , Yin Hong , Leng Xuefeng , Han Yongtao , Zhou Hong 
Published: July 2021

Abstract
Purpose: Esophageal squamous cell carcinoma (ESCC) patients have severe symptom burden after esophagectomy; however, longitudinal studies of symptom recovery after surgery are scarce. This study used longitudinal patient-reported outcome (PRO)-based symptoms to identify severe symptoms and profile symptom recovery from surgery in patients undergoing esophagectomy.Methods: Esophageal cancer patients (N=327) underwent esophagectomy were consecutivly included between April 2019 and March 2020. Data were extracted from the Sichuan Cancer Hospital’s Esophageal Cancer Case Management Registration Database. Symptom assessment time points were pre-surgery and 1, 3, 5, 7, 14, 21, 30, and 90 days post-surgery using the Chinese version of the MD Anderson Symptom Inventory. The symptom recovery trajectories were profiled using mixed-effect models and Kaplan–Meier analysis. Results: The most-servere symptoms after esophagectomy were pain, fatigue, dry mouth, disturbed sleep, and distress. The severity of symptoms peaked on day 1 after surgery. The top two symptoms were fatigue (mean: 5.44[SD 1.88]) and pain (mean: 5.23[SD 1.29]). Fatigue was more severe 90 days after surgery than at baseline (mean: 1.77 [SD 1.47] vs 0.65 [SD 1.05]; P<.0001). Disturbed sleep and distress persisted from pre-surgery to 90 days post-surgery; average sleep recovery time was up to 20 days, and 50.58% of patients had sleep disturbances 90 days post-surgery.Conclusions: Early postoperative pain management after esophagectomy should be considered. Characteristics and intervention strategies of postoperative fatigue, distress, and disturbed sleep in esophageal cancer patients warranty further studies.The study was registered on ChiCTR.org.cn Web site (ChiCTR2000040780, date 12/10/2020).
Spike Protein Recognizer Receptor ACE2 Targeted Identification of Potential Natural Antiviral Drug Candidates Against SARS-CoV-2
Authors: Bouback Thamer A. , Samad Abdus , Nur Suza Mohammad , Abdullah-Al-Mamun Md. , Alam Rahat , Hossen Md Saddam , Nain Zulkar , Imon Raihan Rahman , Ahammad Foysal , Qadri Ishtiaq , Karpiński Tomasz M. 
Published: July 2021

Abstract
Angiotensin-converting enzyme 2 (ACE2), also known as peptidyl-dipeptidase A, belongs to the dipeptidyl carboxydipeptidases family has emerged as a potential antiviral drug target against SARS-CoV-2. Most of the ACE2 inhibitors discovered until now are chemical synthesis; suffer from many limitations related to stability and adverse side effects. However, natural, and selective ACE2 inhibitors that possess strong stability and low side effects can be replaced instead of those chemicals’ inhibitors. To envisage structurally diverse natural entities as an ACE2 inhibitor with better efficacy, a structure-based-pharmacophore model (SBPM) was developed and validated by 20 known selective inhibitors with their correspondence 1166 decoy compounds. The validated SBPM has excellent goodness of hit score and good predictive ability, which has been appointed as a query model for further screening of 11,295 natural compounds. The resultant 23 hits compounds with pharmacophore fit score 75.31 to 78.81 were optimized using in-silico ADMET and molecular docking analysis. Four potential natural inhibitory molecules namely D-DOPA (Amb17613565), L-Saccharopine (Amb6600091), D-Phenylalanine (Amb3940754), and L-Mimosine (Amb21855906) have been selected based onbinding affinity (−7.5, −7.1, −7.1, and −7.0 kcal/mol), respectively. Moreover, 250 ns molecular dynamics (MD) simulations confirmed the structural stability of the ligands within the protein. Additionally, MM/GBSA approach also used to support the stability of molecules to the binding site of the protein that also confirm the stability of the selected four natural compounds. The virtual screening strategy used in this study demonstrated four natural compounds that can be utilized for designing a future class of potential natural ACE2 inhibitor that will block the spike (S) protein dependent entry of SARS-CoV-2 into the host cell.
Study on Sand Liquefaction Induced by Songyuan Earthquake with a Magnitude of M5.7 in China
Authors: Li Ping , Tian Zhaoyang , Bo Jingshan , Zhu Sheng , li Yuying 
Published: July 2021

Abstract
A large-scale sand liquefaction producing typical and novel surface phenomena was found at the epicenter of Songyuan M5.7 earthquake occurring on May 28, 2018. Field survey and experimental test encompassing boring sampling, standard penetration test(SPT), cone penetration test(CPT), scanning electron microscopy(SEM), X-ray diffraction(XRD), and X-ray fluorescence(XRF) were performed to ascertain the liquefaction damage and site characteristic. Cone penetration test is an excellent assay for the identification of liquefied sand layer and acquisition of physio-mechanical parameter. Moreover, the assay is applicable for on-site post-earthquake investigation. Factors promoting the formation and controlling the distribution of the sand liquefaction were analyzed. The liquefaction impacted an 80 km2 area, and was primarily embodied as sand boil and water sprout on rice field, despite producing no significant structural damage. Due to the simple profile of local soil layer, ground motion, geomorphic condition, and groundwater level were the main factors governing the distribution of the liquefaction. Majority of the liquefied sand layer was discovered at the depth less than 10 m. However, deep layer liquefaction at the depth greater than 18 m was also discovered, which was demonstrated by the upward movement of liquefied sand towards the upper silty clay layer at the depth of 17 m. Most importantly, we have identified loess liquefaction, a phenomenon which had not been reported previously in Northeast China. Lastly, it is important to highlight the risk of significant liquefaction damage at Songyuan. Hence, investigating the liquefaction risk is potentially beneficial for augmenting planning on earthquake mitigation, engineering reconnaissance, and design project.
Immunological Characterization of the Chemically Prepared Ghosts of Salmonella Typhimurium as a Vaccine Candidate
Authors: Rabea Sameh , Yassin Aymen S. , Mohammed Aly Fahmy , Salem-Bekhit Mounir M. , Alanazi Fars K. , Esmail Eman Amin , Moneib Nayera A. , Hashem Abd Elgawad M. 
Published: July 2021

Abstract
Background: The purpose of this study is to measure the immunogenic protective response of bacterial ghosts of Salmonella typhimurium in animals. Accordingly, the researchers will be able to do further clinical trials and confirm the efficiency of bacterial ghosts in human vaccination. The targets to be measured are humoral immune response: immunoglobulins elevation (IgG), cellular immune response: granulocytes increment; serum antibacterial activity; success the faeces and liver virulence challenge and High survival rates.Results: The BG vaccine was able to protect 100% of BG subcutaneously vaccinated rats and 75% of BG-adjuvant subcutaneously vaccinated rats. The lowest survival rate was in the BG orally vaccinated group (25%). The maximum level of serum IgG titers as well as serum and faeces bactericidal activity (100% eradication) was exhibited in the subcutaneously vaccinated group with BG-adjuvant followed by the BG subcutaneously vaccinated one. Additionally, the highest granulocytes’ number was observed in the BG-adjuvant subcutaneously immunized group. The bacterial load in liver homogenate eliminated in the subcutaneously vaccinated rats by BG after virulence challenge. Conclusions: The bacterial ghosts of Salmonella enterica serovar Typhimurium that prepared by tween 80 protocol showed an effective vaccine candidate that protected animals, eliminated the virulence in faeces and liver. These findings suggest that chemically induced bacterial ghosts of Salmonella typhimurium can be a promising vaccine.
The Association Between Gut Microbiome Affecting Concomitant Medication and The Related Effectiveness of Immunotherapy in Patients With Stage IV NSCLC
Authors: Verschueren M. V. , Welle C.M. Cramer- van der , Tonn M. , Schramel F. M.N.H. , Peters B. J.M. , de Garde E. M.W. van 
Published: July 2021

Abstract
Objectives
This historically matched cohort study investigated the influence of microbiome-affecting-medication on the effectiveness of immunotherapy in patients with stage IV non-small-cell lung cancer (NSCLC). We postulated that if the effectiveness of immunotherapy is mediated by drug-related changes of the microbiome, a stronger association between the use of co-medication and overall survival (OS) will be observed in patients treated with immunotherapy as compared to patients treated with chemotherapy.
Methods
Immunotherapy patients were matched (1:1) to patients treated with chemotherapy in the pre immunotherapy era. The association between the use of antibiotics, opioids, proton pump inhibitors, metformin and other antidiabetics on OS was assessed with multivariable cox-regression analyses. Interaction tests were applied to investigate whether the association differs between patients treated with immuno- or chemotherapy.
Results
A total of 442 patients were studied. The use of antibiotics was associated with worse OS (adjusted Hazard Ratio (aHR) 1.39, p = 0.02) independent of the type of therapy (chemotherapy or immunotherapy). The use of opioids was also associated with worse OS (aHR 1.33, p = 0.01). The other drugs studied showed no association with OS. Interaction term testing showed no effect modification by immuno- or chemotherapy for the association of antibiotics and opioids with OS.
Conclusion
The use of antibiotics and opioids is similarly associated with worse outcomes in both chemotherapy and immunotherapy treated NSCLC patients. This suggests that the association is likely to be a consequence of confounding by indication rather than disturbing the composition of the microbiome.
Study Protocol for the Exercising Together© Trial: A Randomized, Controlled Trial of Partnered Exercise for Couples Coping with Cancer
Authors: Winters-Stone Kerri , Lyons Karen S. , Dieckmann Nathan , Lee Christopher S. , Mitri Zahi , Beer Tomasz M. 
Published: July 2021

Abstract
Background

Most cancer survivors are married, and cancer strains the physical and mental health of each partner and their intimate relationship. We created a partnered strength training program, Exercising Together©, where the survivor and his/her partner exercise as a team in order to improve physical and mental health of both members of the couple as well as the quality of their relationship. We have not yet determined if Exercising Together© is similarly effective in couples coping with different types of cancer nor if training as a team has unique and added benefits over those derived from supervised group training and/or shared behavior change. The purpose of this study is to determine the unique benefits of Exercising Together© on physical, mental and relational health in couples coping with breast, prostate or colorectal cancer.
Methods

Survivors of prostate, breast and colorectal cancer (N = 294, 98 per cancer site) and their intimate, co-residing partners are recruited to participate in a single-blind, parallel group, randomized trial comparing three exercise groups that train twice per week for 6 months. Couples are randomized to one of three groups: 1) Exercising Together© where partners train as a team in a supervised group setting, 2) separate supervised group exercise classes for survivors or partners, respectively, 2) unsupervised home exercise program provided to each partner. The primary outcome is relationship quality (dyadic coping by the Dyadic Coping scale, emotional intimacy by the Dyadic Adjustment Scale, physical intimacy by the Physical Intimacy Behavior Scale, and symptom incongruence). Secondary outcomes are physical health (% body fat by DXA, serum fasting lipids (triglycerides, HDL and LDL cholesterol), insulin resistance (HOMA-IR), resting blood pressure, C-reactive protein, TNF-alpha, and physical functioning by the short Physical Performance Battery and SF-36) and mental health (depressive symptoms, anxiety, fear of recurrence) of each partner. Outcomes are collected at baseline, mid (3 months), post-intervention (6 months), and follow up (12 months).
Discussion

Exercising Together© could shift the paradigm of survivorship care toward novel couple-based approaches that could optimize outcomes for each partner because their health is interdependent on each other and their relationship.