bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Telomerase Reverse Transcriptase as a Therapeutic Target of Dihydroartemisinin for Esophageal Squamous Cancer
Authors: Li Qingrong , Ma Qiang , Xu Lei , Gao Chuanli , Yao Lihua , Wen Jilin , Yang Miyuan , Cheng Jibing , Zhou Xi , Zou Jiang , Zhong Xiaowu , Guo Xiaolan 
Published: October 2021

Objective: To elucidate the oncogenic role of human telomerase reverse transcriptase (hTERT) in esophageal squamous cancer and unravel the therapeutic role and molecular mechanism of dihydroartemisinin (DHA) by targeting hTERT.Methods: The expression of hTERT in esophageal squamous cancer and the patients prognosis were analyzed by bioinformatic analysis from TCGA database, and further validated with esophageal squamous cancer tissues in our cohort. The Cell Counting Kit-8 (CCK8) and colony formation assay were used to evaluate the proliferation of esophageal squamous cancer cell lines (Eca109, KYSE150, and TE1) after hTERT overexpression or treated with indicated concentrations of DHA. Transwell migration assay and scratch assay were employed to determine the migration abilities of cancer cells. Fluorescence microscopy and flow cytometry were conducted to measure the intracellular reactive oxygen species (ROS) levels in cancer cells after treated with DHA. Moreover, RT-PCR and Western blot were performed to test the alteration of associated genes on mRNA and protein level in DHA treated esophageal squamous cancer cell lines, respectively. Furthermore, tumor-bearing nude mice were employed to evaluate the anticancer effect of DHA in vivo.Results: We found that hTERT was significantly upregulated in esophageal squamous cancer both from TCGA database and our cohort also. Overexpression of hTERT evidently promoted the proliferation and migration of esophageal squamous cancer cells in vitro. Moreover, DHA could significantly inhibit the proliferation and migration of esophageal cancer cell lines Eca109, KYSE150, and TE1 in vitro, and significantly down-regulate the expression of hTERT on both mRNA and protein level in a time- and dose-dependent manner as well. Further studies showed that DHA could induce intracellular ROS production in esophageal cancer cells and down-regulate SP1 expression, a transcription factor that bound to the promoter region of hTERT gene. Moreover, overexpression of SP1 evidently promoted the proliferation and migration of Eca109 and TE1 cells. Intriguingly, rescue experiments showed that inhibiting ROS by NAC alleviated the downregulation of SP1 and hTERT in cells treated with DHA. Furthermore, overexpression of SP1 or hTERT could attenuate the inhibition effect of DHA on the proliferation and migration of Eca109 cells. In tumor-bearing nude mice model, DHA significantly inhibited the growth of esophageal squamous cancer xenografts, and downregulated the expression of SP1 and hTERT protein, while no side effects were observed from heart, kidney, liver, and lung tissues by HE stain.Conclusion: hTERT plays an oncogenic role in esophageal squamous cancer and might be a therapeutic target of DHA through regulating ROS/SP1 pathway.
A Modified Endoscopic Transforaminal Lumbar Interbody Fusion Technique: Preliminary Clinical Results of 96 Cases
Authors: Gong Junfeng , Huang Zheng , Liu Huan , Zhang Chao , Zheng Wenjie , Li Changqing , Tang Yu , Zhou Yue 
Published: October 2021

Background: As a newly emerging technique, endoscopic transforaminal lumbar interbody fusion (Endo-TLIF) has become an increasingly popular procedure of interest. The purpose of this study was to introduce a modified Endo-TLIF system and share our preliminary clinical experiences and outcomes in treating lumbar degenerative disease with this procedure.Methods: Ninety-six patients (thirty-seven men and fifty-nine women; mean age 55.85 ± 11.03 years) with lumbar degenerative diseases who underwent Endo-TLIF in our hospital were enrolled. The surgical time, volume of intraoperative blood loss, postoperative hospitalization time and postoperative drainage were documented. Clinical outcomes were evaluated by visual analog scale (VAS) scores, Oswestry Disability Index (ODI) scores, and modified MacNab criteria. Bone fusion was identified through computerized tomography (CT) scans or X-ray during the follow-up period.Results: All patients were followed up for at least 12 months, and the average follow-up time was 17.03 ± 3.27 months. The mean operative time was 136.79 ± 30.14 minutes, and the mean intraoperative blood loss was 53.06 ± 28.89 ml. The mean VAS scores of low back pain and leg pain were 5.05 ± 1.37 and 6.25 ± 1.03, respectively, before surgery, which improved to 2.27 ± 0.66 and 2.22 ± 0.55, respectively, after the operation (P &lt; 0.05). The final VAS scores of low back pain and leg pain were 0.66 ± 0.60 and 0.73 ± 0.66, respectively (P &lt; 0.05). The preoperative ODI score (49.06 ± 6.66) also improved significantly at the 3-month follow-up (13.00 ± 7.37; P &lt; 0.05). The final ODI score was 8.03 ± 6.13 (P &lt; 0.05). There were 10 cases of non-fusion (nine women and one man) at the 12-month follow-up, but no cases of non-union were identified by imaging at the final follow-up.Conclusions: The present study demonstrated satisfactory clinical and radiologic results among patients who received Endo-TLIF treatment from our institution. This indicates that Endo-TLIF is efficient and safe for select patients.
Selected problems of prematurity and prematurely born child care
Authors: Tataj-Puzyna Urszula , Kondraciuk Karolina , Gotlib Joanna 
Published: October 2021

The evolution of perinatology and the progress of advanced methods of prenatal diagnosis have contributed to the rise of frequency of birth and survival of newborns with very low birth weight, born before 32 weeks of pregnancy. A three-tier perinatology care system in Poland, an increasingly outstanding knowledge of neonatologists, and newer and more advanced equipment for critical care therapy, all provide growingly safer conditions for the life and development of prematurely born children. Prematurity is not only a problem of the preterm baby, but poses a challenge for the family, notably the mother, who must face the challenges of care of the preterm baby. In this article, selected problems of prematurity and care of a preterm baby are presented. The most common causes of preterm birth incidence are reported. The mother’s situation after a preterm birth is defined. Based on the current literature, the profile of preterm babies and the EBM (Evidence-Based Medicine) paradigm, the rules for treatment and care for a preterm baby are presented.
Body image and self-esteem
Authors: Ziółkowska Beata 
Published: October 2021

Introduction: Motherhood is an amazing experience for a woman. It turns out, however, that the joy of having a baby is often accompanied by a negative body image and, at the same time, a reduction in self-esteem.
Method: The study sample consisted of 60 puerperal women. A personal questionnaire was used to collect information related to pregnancy, family situation. The body image was verified with the Body Esteem Scale (BES), and the MSEI Multidimensional Self-Assessment Questionnaire was used to test the self-esteem. The research was conducted in the first three quarters of 2019 in Poland.
Results: 66.7% of the mothers surveyed gave birth without complications, 53.3% breastfed their babies. Among women for whom appearance is very important, the lowest weight gain was observed during pregnancy. The relationship between the body image in all its dimensions and the support obtained from relatives has been proven (p=0.001 to 0.036). It has been proved that the type of feeding the child has a significant relationship with the "weight control" subscale (F=3.03; p=0.04), and the "physical condition" with the assessment of the body before pregnancy (F=4.34; p=0.004). Women giving birth in natural conditions obtained significantly higher results in "competence" and "popularity". "Weight control" negatively correlates with the feeling of "being loved" (r=-0.47; p=0.001), but positively with "leadership abilities" (r=0.31; p=0.016) and with "vitality" (r=0.46, 0.001). Also "physical condition" negatively correlates with the feeling of "being loved" (r=-0.39; p=0.002) and "vitality" (r=-0.45; p=0.001) and "identity integration" (r=-0.31; p=0.018).
Conclusions: The examined women in the puerperium have a much worse image of their body compared to its subjective assessment before the pregnancy. Those of them who declared support from their relatives assessed their own bodies much more favorably. The type of child feeding in the study sample was significantly related to the body image of the mothers in the “weight control” subscale. In mothers who assessed their own body extremely (very good vs. very bad) before delivery, the greatest decrease in the sense of sexual attractiveness was shown.
IDENTIFIKASI HAMA PADA TANAMAN BUNGA ABADI (Xerochrysum bracteatum) DI DISTRIK KURULU KABUPATEN JAYAWIJAYA
Authors: Mahanani Anti Uni , Yikwa Erkius 
Published: October 2021

ABSTRACTThis study aims to determine the pests found in the perennial flower plants. This research was carried out in the eternal flower garden (Xerochrysum bracteatum) Jayawijaya District in Kurulu District. The material used in this study was the perennial flower plant (Xerochrysum bracteatum). The equipment used is a digital camera, a knife blade, a small circle, and stationery. This research was carried out in the eternal flower garden in Kurulu District, Jayawijaya Regency. Next identified the type of pest. This study uses a research method that is direct observation of the research object. The object of research is directed at the population of perennial flower plants that have been planted by farmers in the Kurulu district of Jayawijaya Regency. Pests found in perennial flower plants in Kurulu District Jayawijaya Regency are: Pests Ladybugs (Helopeltis spp); Helopeltis antonii pest; Flower Fly Pests (Syrphus balteatus sp); Pseudosphinx tetrio (Linnaeus) caterpillar; Borer Etiella zinckenella; Rhinoceros Beetle Pests (Oryctes rhinoceros); Pests Taeniothrips simplex; The Ladybug Pug (Andrallus spinidens); Skin Beetle Pests (Dermestidae); Ladybug Pest Lygocoris spp; and Bees (Apis mellifera).Keywords: identification, pests, perennial flower plantsABSTRAKPenelitian ini bertujuan untuk mengetahui hama-hama yang terdapat di tanaman bunga abadi. Penelitian ini dilaksanakan di pertanaman Bunga Abadi (Xerochrysum bracteatum) Kabupaten Jayawijaya di Distrik Kurulu. Bahan yang digunakan dalam penelitian ini adalalah tanaman bunga abadi atau yang disebut dengan Bunga Plastik (Xerochrysum bracteatum). Alat yang digunakan adalah kamera digital, pisau kater, lingkis kecil, dan alat tulis. Penelitian ini dilaksanakan pada pertanaman bunga abadi di Distri Kurulu Kabupaten Jayawijaya. Selanjutnya diidentifikasikan jenis hamanya. Penelitian ini menggunakan metode penelitian yaitu pengamatan langsung terhadap obyek penelitian. Objek penelitian diarahkan pada populasi tanaman bunga abadi yang telah ditanam oleh para petani di distrik Kurulu Kabupaten Jayawijaya. Hama-hama yang terdapat pada tanaman bunga plastik di Distrik Kurulu Kabupaten Jayawijaya adalah : Hama Kepik (Helopeltis spp); Hama Helopeltis antonii; Hama Lalat Bunga (Syrphus balteatus sp); Hama ulat Pseudosphinx tetrio (Linnaeus); Hama Penggerek Etiella zinckenella; Hama Kumbang Badak (Oryctes rhinoceros); Hama Taeniothrips simplex; Hama Kepik Lembing (Andrallus spinidens); Hama Kumbang Kulit (Dermestidae); Hama Kepik Lygocoris spp; dan Lebah (Apis mellifera).Kata kunci : identifikasi, hama, tanaman bunga abadi
TEKNIK BUDIDAYA TANAMAN TOMAT CHERRY (Lycopersicum Cerasiformae Mill ) DI GAPOKTAN LEMBANG JAWA BARAT
Authors: Sari Nurnita , Murtilaksono Aditya 
Published: October 2021

ABSTRACTTomato is a type of vegetable that has several varieties, one of which is a type of tomato cerasiforme (Dun.) Alef. Known as cherry tomatoes which have the characteristics of small and round fruit. Lower tomato production in Indonesia instead of varieties that are not planted are suitable, technical culture is not good, and the conversion of agricultural functions that use agricultural land is increasingly limited. To approve the results of production and community needs for a good tomato plant then began to be asked to cherry tomato planting techniques in Gapoktan Lembang Agri. The research method began with processing, planting, maintenance, harvesting to post-harvest. The results of the Gapoktan Lembang Agri West Java research application of cultivation techniques of cherry tomato plants that produce quality cherry tomatoes are marketed overseas such as SingaporeKeywords : Tomato, West Java, Harvest, Post Harvest ABSTRAKTomat merupakan jenis sayuran buah yang memiliki beberapa jenis varietas salah satunya yaitu jenis varietas tomat cerasiforme (Dun.) Alef. Dikenal dengan tomat cherry yang memiliki ciri khas berbuah kecil dan bulat. Rendahnya produksi tomat di Indonesia diduga disebabkan varietas yang ditanam tidak cocok, kultur teknis yang kurang baik, serta alih fungsi lahan pertanian yang menyebabkan lahan pertanian semakin sempit. Untuk memenuhi hasil produksi dan kebutuhan masyarakat akan tanaman tomat yang baik maka mulai dilakukannya penerapan teknik budidaya tomat cherry di Gapoktan Lembang Agri Jawa Barat. Metode penelitian yaitu mulai dengan melakukan pengolahan lahan, penanaman, pemeliharan, panen hingga pasca panen. Hasil penelitian Gapoktan Lembang Agri penerapan teknik budidaya tanaman tomat cherry yang baik menghasilkan tomat cherry yang berkualitas yang dipasarkan hingga ke luar negeri seperti SingapuraKata Kunci : Tomat, Jawa Barat, Panen, Pasca panen
PENGARUH MODEL STYROFOAM DAN SISTEM HIDROPONIK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PAKCOY (Brassica rapa L.)
Authors: Pudjiwati Eko Hary , Asmina Duta Delon 
Published: October 2021

ABSTRACT Pakcoy's crop production and continuity are predicted to experience obstacles. The solution to overcome these problems is to apply the static hydroponic method. However, the condition of static hydroponic media solutions tends to experience saturation which will inhibit plant growth. This study aims to determine the effect of the modification of the styrofoam model and the hydroponic system on the condition of the static hydroponic media solution and to find out which treatment gives the best effect on growth, yield, and economic value. The results showed that the styrofoam model and the hydroponic system were able to influence the condition of the media solution on the parameters of dissolved oxygen content, pH, water volume and water level. S3H2 treatment provides the best pakcoy plant growth in the parameters of root volume, plant height, top wet weight, and underweight safe weight so that it can provide the best results. Based on the analysis of farming that has been done in five harvests, S1H2 treatment provides the best economic value with an R / C ratio of 1,52 and a B / C ratio of 0,52. Key words: hydroponics, hydroponic systems, media solutions, pakcoy, styrofoam models. ABSTRAK Produksi dan kontinuitas tanaman pakcoy diprediksi akan mengalami hambatan. Solusi untuk mengatasi permasalahan tersebut adalah dengan menerapkan metode hidroponik statis. Namun kondisi larutan media hidroponik statis cenderung mengalami kejenuhan yang akan menghambat pertumbuhan tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh modifikasi model styrofoam dan sistem hidroponik terhadap kondisi larutan media hidroponik statis dan untuk mengetahui perlakuan mana yang memberikan pengaruh terbaik terhadap pertumbuhan, hasil, dan nilai ekonomis. Hasil penelitian menunjukkan bahwa model styrofoam dan sistem hidroponik mampu memengaruhi kondisi larutan media pada parameter kandungan oksigen terlarut, pH, volume air dan tinggi air. Perlakuan S3H2 memberikan pertumbuhan tanaman pakcoy terbaik pada parameter volume akar, tinggi tanaman, berat basah brangkas atas, dan berat basah brangkas bawah sehingga mampu memberikan hasil terbaik. Berdasarkan analisis usaha tani yang telah dilakukan dalam lima kali panen, perlakuan S1H2 memberikan nilai ekonomis terbaik dengan nilai R/C ratio 1,52 dan B/C ratio 0,52. Kata kunci: hidroponik, larutan media, model styrofoam, pakcoy, sistem hidroponik.
PENGGUNAAN 2,4-D UNTUK INDUKSI KALUS KLON KAKAO UNGGUL SULAWESI 1
Authors: Rasud Yulianti , Habil Moh. , Tony Tony 
Published: October 2021

ABSTRACT The multiplication of cocoa clones in conventional Sulawesi has not yet been able to fulfill the demand for large quantities of seeds because it is limited by the number of shoots and branches ready to be tapped, connected and oculated and takes longer to produce large quantities of seeds. One alternative in overcoming this problem is plant proragation using tussue culture techniques. The aim of this experiment was to determine the appropriate of 2,4-D for callus induction of superior cocoa clones Sulawesi via in vitro culture. This experiment used Completely Randomized Design with five treatments, namely 0.50 ppm 2,4-D, 0.75 ppm 2,4-D, 1.00 ppm 2,4-D, 1.25 ppm 2,4-D and 1.50 ppm 2,4-D. Parameters observed consisted of the time, percentage, color and texture of calli. Data was analized by using analysis of variance and differences between mean treatments were determined by Honestly Significant Difference Test at 5% level. Results of this experiment indicated that the ability of different callus induction at various concentrations of 2,4-D for superior cocoa clones in Sulawesi 1 was tried. it was obtained the quickest callus formation at concentration 0.50ppm 2,4-D namely average 4.22 WAC with the percentage of callus formation was up to 99,33%. Keywords: Callus Induction, Clones Sulawesi 1, 2,4-D ABSTRAK Perbanyakan klon kakao Sulawesi secara konvensional saat ini belum dapat memenuhi permintaan bibit dalam jumlah besar karena sangat dibatasi oleh jumlah tunas dan cabang yang siap disetek, disambung, dan diokulasi serta dibutuhkan waktu yang lebih lama untuk menghasilkan bibit dalam jumlah besar. Salah satu alternatif dalam mengatasi masalah tersebut adalah perbanyakan tanaman dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk memperoleh protokol yang tepat dalam menginduksi kalus sebagai upaya awal dalam perbanyakan tanaman kakao melalui embryogenesis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 level perlakuan yaitu 0,50 ppm 2,4-D, 0,75 ppm 2,4-D, 1,00 ppm 2,4-D, 1,25 ppm 2,4-D dan 1,50 ppm 2,4-D. Pengamatan dilakukan terhadap saat muncul kalus, persentase eksplan berkalus, warna kalus dan tekstur kalus. Data diolah dengan analisis ragam dan perbedaan antar perlakuan ditentukan dengan Uji Beda Nyata Jujur pada taraf 5%. Hasil penelitian menunjukkan kemampuan induksi kalus berbeda pada berbagai konsentrasi 2,4-D untuk klon kakao unggul Sulawesi 1 yang dicobakan. Saat muncul kalus paling cepat diperoleh pada konsentrasi 0,5 ppm 2,4-D yaitu rata-rata 16,67 HST dengan persentase pembentukan kalus tertinggi mencapai 99,33%. Selanjutnya, warna dan tekstur kalus yang dihasilkan yaitu remah putih dan remah kecoklatan. Kata Kunci: Induksi Kalus, Klon Sulawesi 1, 2,4-D.
Pengaruh Lama Pemakaian Sediaan Kosmetik Bedak Padat Terhadap Cemaran Mikroba
Authors: Munira Munira , Fardilla Cut , Zakiah Noni , Rasidah Rasidah , Nasir Muhammad 
Published: October 2021

Bedak adalah salah satu sediaan kosmetik yang dipakai dalam jangka waktu yang lama. Bedak yang digunakan akan terjadi kontaminasi bahkan semakin meningkat seiring dengan lamanya pemakaian. Faktor-faktor yang mempengaruhi antara lain penyimpanan, lingkungan, dan spons bedak. Penggunaan spons bedak merupakan salah satu sumber cemaran bakteri. Di mana penggunaan spons yang sama secara berulang-ulang dan bersentuhan langsung pada kulit dapat meningkatkan jumlah mikroba. Tujuan penelitian ini adalah untuk mengetahui pengaruh lamanya penyimpanan, pemakaian suatu sediaan kosmetik bedak padat terhadap tingkat cemaran mikroba. Sampel yang diuji adalah bedak padat yang belum dipakai dan yang telah dipakai selama 2 minggu. Hasil penelitian berupa jumlah cemaran bakteri (ALT) dibandingkan dengan syarat menurut Peraturan Kepala Pengawasan Obat dan Makanan RI No.HK.03.1.23.07.11.6662 Tahun 2011 yaitu tidak boleh melebihi 103 koloni/g. Hasil uji cemaran bakteripada sediaan bedak padat sebelum pemakaian mempunyai nilai rata-rata ALT 2,6 x 101koloni/g dan sesudah pemakaian mempunyai nilai rata-rata ALT 9,2 x 101 koloni/g. Nilai ALT yang diperoleh dari masing-masing sediaan kosmetik bedak padat telah memenuhi syarat.Hasil uji t dependen menunjukkan adanya perbedaan jumlah bakteri pada bedak padat sebelum dipakai dengan setelah dipakai selama 2 minggu (t = 0.000). Kata Kunci: Kosmetik, bedak padat, Angka Lempeng Total ABSTRACTPowder is one of the cosmetic preparations used for a long time. Powder that is used will occur contamination even increasing along with the length of usage. This can be caused by several factors including storage, environment, powder sponges and others. The use of powder sponges is one source of bacterial contamination. Where the use of the same sponge repeatedly and in direct contact with the skin can increase the number of microbes. The objective of study was to determine the effect of the duration of use of a compact powder cosmetic preparation on the level of microbial contamination. The samples tested were solid powder that has not been used and that has been used for 2 weeks. The results of the study were the Total Plate Count (TPC) compared to the Regulation of the Head of the Republic of Indonesia Drug and Food Control Number HK.03.1.23.07.11.6662 of 2011 concerning the Requirements for Microbial Pollution and Heavy Metals in Cosmetics which should not exceed 103 colonies / g. The test results of bacterial contamination on solid powder preparations before use have an average value of ALT 2,6 x 101 colony / g and after use have an average ALT value of 9.2 x 101 colony / g. The ALT value obtained from each compact powder cosmetic preparation has met the requirements. The results of the dependent t test showed a difference in the number of bacteria on solid powder before use with after being used for 2 weeks (t = 0.000). Keywords: Cosmetics, Compact Powder, Total Plate Count