bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of Wetland Plants on Microbial Community and Methane Metabolisms
Authors: Waldo Nicholas B , Chistoserdova Ludmila , Hu Dehong , Gough Heidi L. , Neumann Rebecca B. 
Published: April 2021

Abstract
Aims

Microbial activity in the soil of wetlands is responsible for the emission of more methane to the atmosphere than all other natural sources combined. This microbial activity is heavily impacted by plant roots, which influence the microbial community by exuding organic compounds and by leaking oxygen into an otherwise anoxic environment. This study compared the microbial communities of planted and unplanted wetland soil from an Alaskan bog to elucidate how plant growth influences populations and metabolisms of methanogens and methanotrophs.
Methods

A common boreal wetland sedge, Carex aquatilis, was grown in the laboratory and DNA samples were sequenced from the rhizosphere, unplanted bulk soil, and a simulated rhizosphere with oxygen input but no organic carbon.
Results

The abundance of both methanogens and methanotrophs were positively correlated with methane emissions. Among the methanotrophs, both aerobic and anaerobic methane oxidizing microbes were more common in the rhizosphere of mature plants than in unplanted soil, while facultative methanotrophs capable of utilizing either methane or other molecules became relatively less common.
Conclusions

These trends indicate that roots create an environment which favors highly specialized microbial metabolisms over generalist approaches. One aspect of this specialized microbiome is the presence of both aerobic and anaerobic metabolisms, which indicates that oxygen is present but is a limiting resource controlling competition.
Spidroin Striped Micropattern Promotes Chondrogenic Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells
Authors: Barlian Anggraini , Saputri Dinda Hani’ah Arum , Hernando Adriel , Prajatelistia Ekavianty , Tanoto Hutomo 
Published: April 2021

Abstract
Cartilage tissue engineering, particularly micropattern, can influence the biophysical properties of mesenchymal stem cells (MSCs) leading to chondrogenesis. In this research, human Wharton’s jelly MSCs (hWJ-MSCs) were grown on a striped micropattern containing spider silk protein (spidroin) from Argiope appensa. This research aims to direct hWJ-MSCs chondrogenesis using micropattern made of spidroin bioink as opposed to fibronectin that often used as the gold standard. Cells were cultured on striped micropattern of 500 µm and 1000 µm width sizes without chondrogenic differentiation medium for 21 days. The immunocytochemistry result showed that spidroin contains RGD sequences and facilitates cell adhesion via integrin β1. Chondrogenesis was observed through the expression of glycosaminoglycan, type II collagen, and SOX9. The result on glycosaminoglycan content proved that 1000 µm was the optimal width to support chondrogenesis. Spidroin micropattern induced significantly higher expression of SOX9 mRNA on day-21 and SOX9 protein was located inside the nucleus starting from day-7. COL2A1 mRNA of spidroin micropattern groups was downregulated on day-21 and collagen type II protein was detected starting from day-14. These results showed that spidroin micropattern enhances chondrogenic markers while maintains long-term upregulation of SOX9, and therefore has the potential as a new method for cartilage tissue engineering.
LncRNA ASAP1-IT1 Enhances Cancer Cell Stemness via Regulating miR-509-3p/YAP1 Axis in NSCLC
Authors: Liu Yantao , Yang Yuping , Zhang Lingli , Lin Jiaqiang , Li Bin , Yang Min , Li Honghui , Chen Kangwu , Zhao Wei 
Published: April 2021

Abstract
Background: Non-small cell lung cancer (NSCLC) is a major cause of cancer-related death worldwide, and cancer stem cell is mainly responsible for the poor clinical outcome of NSCLC. Although previous reports indicated long noncoding RNAs (lncRNAs) play important roles in cancer stemness maintain, the underlying causes mechanisms remain is still mysterious. Our study aims to investigate the role of ASAP1 Intronic Transcript 1 (ASAP1-IT1) in cancer cell stemness of NSCLC. Methods: qRT-PCR analysis the expression of ASAP1-IT1 and microRNA-509-3p (miR-509-3p) in NSCLC tissues, cancer cells and spheres of cancer cells-derived cancer stem cells. Knockdown of ASAP1-IT1 or overexpression of miR-509-3p in NSCLC cells by infection or transfection. Sphere formation and colony formation detects NSCLC stem cell-like properties and tumor growth in vitro. Luciferase reporter assays, RNA immunoprecitation (RIP) and qRT-PCR assays analyzed the interaction between lncRNA and miRNA, qRT-PCR and Western blot detect ASAP1-IT1/miR-509-3p axis's regulation on the expression of regulated genes. NSCLC xenograft mice model validates ASAP1-IT1 role in NSCLC stemness and tumor growth in vivo. Results: ASAP1-IT1 was up-regulated in NSCLC tissues, cancer cells, and in spheres of A549-derived cancer stem cells. Downregulated ASAP1-IT1 or miR-509-3p significantly decreased cell colony formation and stem cell-like properties of A549-dereived stem cells with decreased expression of stem cell biomarkers SOX2, CD34, and CD133, and suppressing the expression of cell growth-related genes, Cyclin A1, Cyclin B1, and PCNA. Furthermore, knockdown of ASAP1-IT1 or overexpression of miR-509-3p repressed tumor growth in nude mice via reducing expression of tumorigenic genes. ASAP1-IT1 was found to interact with miR-509-3p. Moreover, overexpression of ASAP1-IT1 blocked miR-509-3p mediated regulation of stem cell-like properties, cell growth, and cell apoptosis of A549-dereived stem cells both in vitro and in vivo. Finally, the level of YAP1 was regulated by ASAP1-IT1 and miR-509-3p.Conclusions: YAP1-involved ASAP1-IT1/miR-509-3p axis promoted NSCLC progression by regulating cancer cell stemness，and targeting related signaling is a promising therapeutic strategy to overcome NSCLC stemness.
Prevalence of Cachexia After Curative Cisplatin-Based Concurrent Chemoradiation in Head and Neck Cancers
Authors: Silva Willian das Neves , Rivelli Thomas Giollo , Simao Eduardo Furquim , Kulcsar Marco Aurelio Vamondes , Junior Gilberto de Castro 
Published: April 2021

Abstract
PurposeWe determine the frequency of cachexia among head and neck squamous cell carcinoma patients treated with cisplatin-based chemoradiation with curative intent and presenting no evidence of disease. MethodsConsecutive patients were included from January 2014 to February 2017. Participants were over 18 y.o. and diagnosed with head and neck squamous cell carcinomas previously treated with definitive or adjuvant chemoradiation. Eligible patients were in regular follow-up for at least 2 years, with no evidence of disease. Body weight, height, mid-arm muscle circumference, muscle strength, and nutritional status were measured, and blood tests were obtained. The main outcome was the presence of cachexia, and self-reported dysphagia. Results120 patients were included, 73% were male, and age was 59 y.o. (range, 21–78). The most common primary site was oropharynx (42%). Median follow-up was 42 mo. (range, 24–125 mo.). Most patients presented locally advanced disease at diagnosis: 73% T3-T4 and 72% N+. Dysphagia was a major complaint (73%). Cachexia was diagnosed in 23 (20.7%) and 10 (8.6%) patients according to Fearon and Evans criteria, respectively. Cachectic patients presented lower mid-arm muscle circumference (p < 0.05). In addition, lower muscle strength levels (p<0.05) were found among cachectic patients according to Evans criteria, and there was an association between the presence of dysphagia and the diagnosis of cachexia. ConclusionsHead and neck squamous cell carcinoma patients with no evidence of disease frequently present cachexia after chemoradiation in a long-term follow-up. More effective preventive and therapeutic strategies for cachexia are required in this scenario.
Biosorption and Biodegradation of Anthraquinone Based Dye (Indanthrene Blue RS) by the Immobilized Microbial Consortium in a Continuous Packed bed Bioreactor Using Corn-Cob Biochar
Authors: Mohanty Swati Sambita , Kumar Arvind 
Published: April 2021

Abstract
The current study describes the aerobic biodegradation of Indanthrene Blue RS dye by a microbial consortium immobilized on corn-cob biochar in a continuous up-flow packed bed bioreactor. The adsorption experiments were performed without microbes to monitor the adsorption effects on initial dye decolorization efficiency. The batch experiments were carried out to estimate the process parameters, and the optimal values of pH, temperature, and inoculum volume were identified to be 10.0, 30 ºC, and 3.0 × 106 CFU mL-1, respectively. During the continuous operation, the effect of flow rate, initial substrate concentration, inlet loading rate of Indanthrene Blue RS on the elimination capacity, and its removal efficiency in the bioreactor was studied. The continuous up-flow packed bed bioreactor was performed at different flow rates (0.25 to 1.25 L h-1) under the optimal parameters. The maximum removal efficiency of 90% was observed, with the loading rate varying between 100 to 300 mg L-1 d-1. The up-flow packed bed bioreactor used for this study was extremely useful in eliminating Indanthrene Blue RS dye using both the biosorption and biodegradation process. Therefore, it is a potential treatment strategy for detoxifying textile wastewater containing anthraquinone based dyes.
In vivo monoclonal antibody efficacy against SARS-CoV-2 variant strains
Authors: Diamond Michael , Chen Rita , Winkler Emma , Case James , Aziati Ishmael , Bricker Traci , Joshi Astha , Darling Tamarand , Ying Baoling , Errico John , Shrihari Swathi , VanBlargan Laura , Xie Xuping , Gilchuk Pavlo , Zost Seth , Droit Lindsay , Liu Zhuoming , Stumpf Spencer , Wang David , Handley Scott , Stine W , Shi Pei-Yong , Garcia-Knight Miguel , Andino Raul , Chiu Charles , Ellebedy Ali , Fremont Daved , Whelan Sean , Crowe James , Purcell Lisa , Corti Davide , Boon Andrianus 
Published: April 2021

Abstract
Rapidly-emerging variants jeopardize antibody-based countermeasures against SARS-CoV-2. While recent cell culture experiments have demonstrated loss of potency of several anti-spike neutralizing antibodies against SARS-CoV-2 variant strains1-3, the in vivo significance of these results remains uncertain. Here, using a panel of monoclonal antibodies (mAbs) corresponding to many in advanced clinical development by Vir Biotechnology, AbbVie, AstraZeneca, Regeneron, and Lilly we report the impact on protection in animals against authentic SARS-CoV-2 variants including WA1/2020 strains, a B.1.1.7 isolate, and chimeric strains with South African (B.1.351) or Brazilian (B.1.1.28) spike genes. Although some individual mAbs showed reduced or abrogated neutralizing activity against B.1.351 and B.1.1.28 viruses with E484K spike protein mutations in cell culture, low prophylactic doses of mAb combinations protected against infection in K18-hACE2 transgenic mice, 129S2 immunocompetent mice, and hamsters without emergence of resistance. Two exceptions were mAb LY-CoV555 monotherapy which lost all protective activity in vivo, and AbbVie 2B04/47D11, which showed partial loss of activity. When administered after infection as therapy, higher doses of mAb cocktails protected in vivo against viruses displaying a B.1.351 spike gene. Thus, many, but not all, of the antibody products with Emergency Use Authorization should retain substantial efficacy against the prevailing SARS-CoV-2 variant strains.
Experiments on the symmetry of length and time
Authors: Chunhong Li 
Published: April 2021

Abstract
The F - t curve obtained from the process of applying and releasing force to the piezoelectric sensor shows that in the atomic scale, the time coordinate is equivalent to the position coordinate. The time-position coordinate relationship calculated by the experimental data is consistent with the geometric unit obtained in the general theory of relativity, thus the experiment verifies the symmetry of length and time,and connection between the microscopic - quantum mechanics and the macroscopic - general theory of relativity, and a new method for calculating the speed of light is obtained.
Avoidance of the plant hormone cis-jasmone by Aedes aegypti depends on mosquito age in both plant and human odor backgrounds
Authors: Boer Jetske Gudrun de , Kuiper Aron P.S. , Groot Joeri , Loon Joop J.A. van 
Published: April 2021

Abstract
Adults of many mosquito species feed on plants to obtain metabolic energy and to enhance reproduction. Mosquitoes primarily rely on olfaction to locate plants and are known to respond to a range of plant volatiles. We studied the olfactory response of the yellow fever mosquito Aedes aegypti to methyl jasmonate (MeJA) and cis-jasmone (CiJA), volatile compounds originating from the octadecanoid signaling pathway that plays a key role in plant defense against herbivores. Specifically, we investigated how Ae. aegypti of different ages responded to elevated levels of CiJA in two attractive odor contexts, either derived from Lima bean plants or from human skin. Aedes aegypti females landed significantly less often on a surface with CiJA and MeJA compared to the solvent control, CiJA exerting a stronger reduction in landing than MeJA. Odor context (plant or human) had no significant main effect on the olfactory responses of Ae. aegypti females to CiJA. Mosquito age significantly affected the olfactory response, older females (7–9 d) responding more strongly to elevated levels of CiJA than young females (1–3 d) in either odor context. Our results show that avoidance of CiJA by Ae. aegypti is independent of odor background, suggesting that jasmonates are inherently aversive cues to these mosquitoes. We propose that avoidance of plants with elevated levels of jasmonates is adaptive to mosquitoes to reduce the risk of encountering predators that is higher on these plants, i.e. by avoiding ‘enemy-dense-space’.
Iran Drought Monitoring in April 2021
Authors: Sorkhabi Omid Memarian 
Published: April 2021

Abstract
About a third of the world's continents and large parts of Iran are facing drought, which is hampering water supply and water supply management. The problem of drought is a natural phenomenon that causes water shortages in many countries every year. Currently, about 80 countries are located in arid and semi-arid regions of the world and Iran is one of these countries with an average rainfall of about 250 mm. In this study, a new drought index called OMI based on GRACE-FO total water storage (TWS) observations is developed in April 2021. The northern and northwestern regions of Iran have lost about 0.32 meters of TWS. Central and eastern Iran have lost about 0.18 meters of TWS. The southwestern regions of Iran are in a better situation this month than other regions. The eastern and southeastern regions are very mid drought, the northern, northeastern, and northwestern regions are very severe drought. The southeastern areas are moderately drought.
Molecualr Characterization And Varietal Identification For Multiple Abiotic Stress Tolerance In Rice (Oryza sativa L.)
Authors: Ali Alif , R. Beena , Manikanta Chennamsetti Lakshmi Naga 
Published: April 2021

Abstract
Coexistence of two or more abiotic stresses is common in most of the rainfed lowland and upland rice growing areas of India and worldwide. Rice production under these conditions is not sustainable. Identification and development of multiple abiotic stress tolerant rice varieties are to be addressed. Here we tried to identify multiple abiotic stress tolerant varieties from a collection of earlier identified varieties for single stress and validated the known SSR markers for stress tolerance. Twenty rice genotypes were evaluated for individual abiotic stress such as drought, salinity and temperature initially and the tolerant three genotypes in each case were further evaluated for combination of stresses various physio-morphological and biochemical parameters were recorded . Among the genotypes evaluated for combination of stresses, PTB-7 was found to be tolerant for drought and salinity, Nagina-22 was tolerant against temperature and salinity. However, the seeds did not germinate in the presence of all three stresses simultaneously.. Twenty rice varieties viz ., Chomala, MO-16, PTB-35, PTB-60, PTB-39, PTB-55, PTB-30, PTB-7, CRdhan307, Apo, Vyttila-3, Vyttila-4, Vyttila-5, Vyttila-6, Vyttila-7, Vyttila-8, Vyttila-9, Vyttila-10, Nagina-22, and NL-44 were further investigated using microsatellite markers to confirm the genotypic level of tolerance to combination of abiotic stresses. Rice genotypes were screened using 30 reported simple sequence repeat (SSR) markers that are linked to drought, salinity and temperature. Molecular marker analysis of rice genotypes also confirmed that RM8904 and RM1287 were associated with salinity tolerance, RM2612, RM6100 and RM5749 were linked to high temperature tolerant trait. Population analysis also revealed that there is five subpopulation among rice genotypes.
Role of GLP-1 Analogs in the Management of Diabetes and its Secondary
Complication
Authors: Sivakumar Ponnurengam Malliappan , Premkumar B. , Prabhawathi Veluchamy , Prabhakar Pranav Kumar 
Published: April 2021

Abstract:
The cardiovascular complications of type 2 diabetes mellitus (T2DM) including myocardial
infarction, heart failure, peripheral vascular disease and, stroke and retinopathy, nephropathy and neuropathy
are microvascular complications. While the newer therapies like glitazones or even dipeptidylpeptidase-
IV (DPP-IV) inhibitors increase the risk of therapy, the Glucagon Like Peptide-1 Receptor
Agonists (GLP-1RAs), were reported as suitable alternates. The GLP-1RAs reduce major adverse cardiovascular
events (MACE), have anti-atherogenic potential, and possess pleiotropic activity. The
GLP-1RAs were found to improve neuroprotection, enhance neuronal growth, reduce the incidence of
stroke, and improve central insulin resistance. The GLP-1RAs are beneficial in improving the glycemic
profile, preventing macroalbuminuria and reducing the decline in eGFR and enhancing renal protection.
The renal benefits of add-on therapy of GLP-1RAs with SGLT-2 inhibitors have composite renal
outcomes such as suppression of inflammatory pathways, improvement in natriuresis, diuresis, found
to be nephroprotective. Improvement in glycemic control with a reduction in body weight and intraglomerular
pressure and prevention of tubular injury makes the GLP-1RAs as suitable add-on therapies
in improving cardiorenal outcomes. Obesity, an important contributor to insulin resistance and a reduction
in weight, is an essential therapeutic option in addressing diabetic-obesity. It also reduces the
damage to blood-retinal-barrier, thus beneficial in halting the development of diabetic retinopathy. In
diabetic complications, glycemic control, addressing insulin resistance through weight loss, controlling
atherosclerosis through anti-inflammatory effects and cardio-renal-neuro protection, makes GLP-1RAs
a suitable therapeutic strategy on long-term treatment of T2DM. This review discusses the role of
GLP-1RAs in diabetes, the dosage, mono or combination therapy with other antidiabetics in long-term
treatment and its effect in uncontrolled diabetes.
Applications of 3-Substituted 2-Alkoxy- and 2-Alkylthiopropenals in
Organic Synthesis
Authors: Vchislo Nadezhda V. , Verochkina Ekaterina A. 
Published: April 2021

:
α-Functionally substituted α,β-unsaturated aldehydes belong to the highly reactive class of
compounds. They are used as versatile building blocks in organic synthesis. Due to the presence of
several reactive sites in their structure, α,β-unsaturated aldehydes are widely employed as precursors
of various acyclic and heterocyclic compounds, as well as complex natural products. At the same
time, the acrylic systems with heteroatomic substituents (OAlk, SAlk) in the α-position are poorly
studied. Therefore, it is impossible to establish the distribution of electron density reliably and evaluate
the real reactivity of each new representative of this class of compounds. This minireview summarizes
the works demonstrating the broad applicability of 3-substituted 2-alkoxy and 2-
alkylthiopropenals in organic synthesis.
A Review on Machine Learning for Earth Observation Satellite Mission
Planning
Authors: Li Zhiliang , Jiang Mingyong , Ran Da , Zheng Fengjie 
Published: April 2021

:
Mission planning is an integral process that enables earth observation missions and
defines their success rate. This paper identifies key problems and opportunities in machine
learning for earth observation satellite mission planning. Firstly, the description elements,
classification methods, solution process and solution difficulties of the mission planning problem
of earth observation satellite are described. Secondly, the current research status of machine
learning for the earth observation satellite mission planning is summarized and analyzed. Finally,
the problems of current research are analyzed, and the prospect of new field research is given in
light of the development needs.
Click, Pan, Scroll. Una base de datos de 500 sitios web de arquitectura
Authors: Liñán Lluís Juan , González de Mendoza Nicolás Maruri 
Published: April 2021

<p class="p2">Este artículo recopila y analiza 500 sitios web dedicados a la arquitectura. Su finalidad es revelar la constitución y las características generales del espacio donde se distribuye el conocimiento arquitectónico en la World Wide Web. Los datos arrojados por este análisis dibujan un heterogéneo espacio informacional que se encuentra dominado por un limitado número de plataformas que se actualizan a diario, ofrecen algún tipo de participación y acumulan una cantidad expansiva de contenidos.<span class="Apple-converted-space"> </span></p>
Ciudad esponja. Un escenario pospandémico
Authors: Krieger Peter 
Published: April 2021

<p class="p2">Este texto ofrece una revisión del concepto de ciudad esponja, con el que se busca mitigar la crisis del agua y el calentamiento exponencial de muchas megalópolis. Toma como ejemplo dos zonas de la Ciudad de México, donde analiza los parámetros de este concepto con el objetivo de generar un conocimiento que sirva de orientación a los debates sobre la ciudad pospandémica, en especial la necesaria renegociación conceptual del complejo y conflictivo vínculo entre ciudad y naturaleza. El presente estudio subraya la importancia de los suelos para la planeación urbano-arquitectónica, a la vez que promueve la expansión de la infraestructura azul y verde en los densos paisajes urbanos.<span class="Apple-converted-space"> </span></p>
O CORPO COMO DISCURSO AUTOBIOGRÁFICO
Authors: Machado Vinicius Torres , Paiva Giovanna Galisi 
Published: April 2021

A afirmação do real tem sido um campo de debate nas artes da cena que trabalham com aspectos performativos. Dentre os diversos modos de se trabalhar o real observa-se em profusão um tipo de teatro denominado autobiográfico. Esse teatro parece ter como impulso a elaboração de traumas individuais que dialogam com questões de interesse coletivo. O presente artigo percorre brevemente os paradigmas que envolvem a discussão do gênero autobiográfico na literatura e sua transposição para o teatro. O artigo propõe que a base autorreferencial no teatro autobiográfico se afirme não apenas pelas palavras, mas pelo corpo, que é a afirmação de uma memória física. A cicatriz torna-se o paradigma de um trauma sofrido pelo corpo e que não precisa da palavra como único intermediário artístico. Palavras-chaveAutobiografia. Teatros do Real. Corpo. Trauma. Experiência.