bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Genome-wide Simple Sequence Repeat Markers from Whole-genome Sequence of Mungbean (Vigna radiata)
Authors: Mayalagu Kanimoli Mathivathana , Adhimoolam Karthikeyan , Nallathambi Jagadeeshselvam , Rajagopalan Veera Ranjani , Balasubramanian Madhumitha , Shihabdeen Madiha Natchi Samu , Natesan Senthil , Muthurajan Raveendran , Manickam Sudha 
Published: August 2021

Mungbean is an important pulse crop and it is mainly cultivated in Asia for human consumption. Its small genome and diploid nature make it a well-suited model organism among legume crops. Thus, cost-effective, reliable and highly polymorphic molecular markers distributing the whole genome are needed for diversity, mapping and functional genomics studies in this model species. The whole-genome sequence of mungbean was obtained and used as a source of identification of simple sequence repeats (SSR). The sequence reads were aligned and SSRs detection was performed using the Phobos plugin tandem repeat finder in the Geneious software program. A total of 12 mungbean genotypes were selected to validate the newly developed SSR markers. In the present study, a total of 12, 49,774 and 11, 86, 386 perfect and imperfect SSR repeats were identified from the mungbean genome. The tri-repeats were the most abundant (26.10%), followed by hexa (20.82%), penta (20.45%), tetra (17.65%) and di-repeats (14.95%). We designed 1330 SSR primers based on the genomic sequence of flanking perfect SSRs (Di and tri-repeats). Among them, 50 SSR primers uniformly distributed across the 11 mungbean chromosomes were selected and used to validate 12 mungbean genotypes. The newly developed genomic SSR markers generated in the present study are a valuable genomic resource for the mungbean breeding programs.
Chrononutrition and Breast Milk: A Review of the Circadian Variation of Hormones Present in Human Milk
Authors: Moyo Gugulethu T. , Thomas-Jackson Shera C. , Childress Allison , Dawson John , Thompson Leslie D. , Oldewage-Theron Wilna 
Published: August 2021

IntroductionBreast milk contains a wide range of hormones, nutrients, and bioactive compounds. Chrononutrition looks at the interaction between food, both meal timing and meal composition, and circadian rhythms.MethodThis review is a continuation on series on chrononutrition and breast milk. All human studies published in English were included regardless of location or year of publication.ResultsThis review identifies the hormonal circadian variation researched in breast milk to date for melatonin, cortisol, cortisone, leptin, and insulin.ConclusionThe circadian variation observed in some hormones reinforces the superiority of breast milk over formula milk. Melatonin has been the most studied, but more research for all the hormones is needed due to various gaps in the literature and varying levels of study quality. There is insufficient evidence to make recommendations specifically targeting the lactating mother, but we identify and recommend several ideas for future research.
THE CORRELATION BETWEEN WATER, SANITATION, AND HYGIENE WITH SOIL-TRANSMITTED HELMINTHS INFECTION AMONG ELEMENTARY SCHOOL CHILDREN OF ARU ISLANDS DISTRICT, MALUKU
Authors: Konstantin Tiffany , Tantular Indah Setyawati , Athiyyah Alpha Fardah , Rossyanti Lynda 
Published: August 2021

ABSTRACTSoil-Transmitted Helminths (STH) infection is the most common intestinal parasite infection in Indonesia. STH infection can interfere with child development and cause cognitive impairment in severe cases. The school-age group is the most susceptible to STH infection because of direct contact with soil in the school yard. STH infection incidence can be avoided by adequate water, healthy latrines, and good hygiene and can be improved by proper interventions. This study aimed to analyze the correlation between Water, Sanitation, and Hygiene (WASH) with STH infection in Aru Islands District, Maluku. This study used a cross-sectional design and was conducted in Elementary Schools in Karangguli and Wokam villages, Aru Islands district, Maluku. One hundred and six stool samples were collected from both villages. The sociodemographic and WASH data were collected using the interview method. Stool samples were collected and examined for STH infection using a direct smear technique on the light microscope. Data statistical analysis was performed by the SPSS program using the Chi-square or Fischer’s Exact test. The examination results showed that 73 students (68.9%) were infected with STH. The bivariate analysis showed that water source (p = 0.000002), defecation place (p = 0.002), and washing hands after defecation (p = 0.048) were all significantly correlated with STH infection. This study concluded that WASH variables which were water source, defecation place, and washing hands after defecation had a significant correlation with STH infection. Keywords: Water, Sanitation, and Hygiene (WASH); soil-transmitted helminths infection; elementary school students.
FACTORS RELATED TO BREASTMILK PRODUCTION ON POSTPARTUM MOTHERS IN EAST PONTIANAK, WEST KALIMANTAN
Authors: Widyastutik Otik , CHARTASIM YUWAN , TRISNAWATI ELLY , SELVIANA SELVIANA 
Published: August 2021

ABSTRACTProducing breastmilk is a mother’s ability for the next six weeks after giving birth. Based on data from the Health Office of Pontianak City, exclusive breastfeeding coverage is 25% in East Pontianak District. A preliminary study conducted in East Pontianak Sub district, seven out of ten respondents who underwent breastfeeding, their breastmilk was not running well. There are 70% of babies experienced weight increment below 500 grams/month. In addition, 70% mothers drinking less than twelve glasses/day, 70% mothers do not receive support from their husbands in breastfeeding process, 60% mothers have never been exposed to breastfeeding information, and 50% mothers experienced moderate anxiety levels after giving birth. The research aimed to determine the determinants of postpartum mother's milk production in East Pontianak District. This research is observational research with a cross-sectional approach, and the research subject is 48 postpartum mothers. The results showed that the determinant factor could be seen from the relationship of fluid intake (p-value = 0.000), husband's support (p-value=0.000), information exposure (p-value=0.010), supplement (p-value=0.000), and energy intake (p-value=0.000), to breast milk production. Recommendations addressed to the community health center such as work more active in providing counselling, activating cadres by providing practices. Therefore, they can assist in providing knowledge about breastfeeding and making creative promotional media.Keywords : breast milk production, postpartum, nutrition intake
Uji Stabilitas Formulasi Hand and Body Cream Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus lemairei)
Authors: Nurfita Erviana , Mayefis Delladari , Umar Salman 
Published: August 2021

Pendahuluan: Kulit buah naga merah (Hylocereus lemairei) memiliki potensi sebagai bahan baku kosmetika, karena memiliki kandungan antioksidan yang cukup tinggi yang dapat dimanfaatkan sebagai pelembab kulit dan mencegah kulit kering. Tujuan: Penelitian ini bertujuan untuk mengetahui stabilitas fisik ekstrak kulit buah naga merah (Hylocereus lemairei) yang diformulasikan menjadi sediaan krim yang dapat dimanfaatkan sebagai pelembab pada tangan maupun seluruh badan. Metode: Krim dibuat dalam 3 seri konsentrasi yaitu 4%, 6% dan 8% serta basis krim sebagai kontrol negatif. Pengujian stabilitas krim meliputi, organoleptis, homogenitas, tipe krim, uji pH, daya sebar, viskositas, uji iritasi kulit, uji kesukaan serta uji stabilitas sediaan meliputi uji cycling test, uji mekanik, dan uji stabilitas selama penyimpanan pada suhu rendah (4 ± 2°C) dan suhu kamar (30°C). Hasil: Hasil penelitian menunjukkan bahwa ekstrak kulit buah naga merah (Hylocereus lemairei) dapat diformulasikan dalam bentuk krim serta memiliki kestabilan yang cukup baik selama proses penyimpanan. Kesimpulan: Ekstrak kulit buah naga merah (Hylocereus lemairei) memiliki kestabilan yang baik sebagai sediaan krim yang dapat digunakan sebagai pelembab kulit tangan dan seluruh tubuh.
Aphrodisiac Activity of Ethanolic Extracts from the Fruits of Three Pepper Plants from Piperaceae Family
Authors: Kusumawati Idha , Mahatmaputra Syailendra , Hadi Rohman , Rohmania Rohmania , Rullyansyah Subhan , Yusuf Helmy , Rahman Abdul 
Published: August 2021

Background: Pepper plants belong to the Piperaceae family with many pharmacological activities. The fruits of these plants have been widely used traditionally for various therapies, one of which is an aphrodisiac. Objective: The purpose of this study was to determine the aphrodisiac activity of the fruits of three pepper plants, Piper nigrum, Piper retrofractum, and Piper cubeba to prove their traditional use. Methods: The level of piperin, the active compound of plants from the family Piperaceae, was also determined in the ethanolic extract of those three fruits of the pepper plant. The aphrodisiac activity was determined by counting the number of introducing and mounting mice. The acute toxicity test of the extract was carried out according to the OECD-423 guidelines. Results: The ethanolic extract of Piper nigrum fruits had the highest piperin concentration, while the ethanolic extract of Piper retrofractum fruits had the highest aphrodisiac activity, according to the findings. During the entire duration of the acute toxicity study, no signs of toxicity or mortality were discovered.Conclusion: This study proves that all fruits of three species of pepper plants exhibit aphrodisiac activity. Furthermore, this study also shows that not only piperin is responsible for the aphrodisiac effect.
An Immunometabolic Pathway for Hyperglycemia?-Commentary on: Packed Red Blood Cells Inhibit T-Cell Activation via ROS-Dependent Signaling Pathways
Authors: Papadopoulos Charalampos , Anagnostopoulos Konstantinos , Tentes Ioannis 
Published: August 2021

Abstract
In this commentary we discuss the novel findings of Gerner et al. In their study, it was found that red blood cells inhibit T cell activation through scavenging of reactive oxygen species. We propose a novel immunometabolic pathway, based on previous studies which showed that hyperglycemia abolished the antioxidant function of red blood cells.
Тhe impact of alcohol abuse on the development of non-communicable diseases (analytical review)
Authors: Kaigorodova T. V. , Kryukova I. A. 
Published: August 2021

The harmful use of alcohol is a risk factor for the development of non-communicable diseases (NCDs), such as cancer, cardiovascular disease, gastrointestinal diseases, diabetes and others. In addition to morbidity, alcohol abuse increases mortality, especially at young ages. An important characteristic is the frequency and amount of alcohol consumed by a person. The more often and more a person abuses alcohol, the higher the risk of developing NCDs and the mortality rate. Purpose of the study: analysis of publications of the World Health Organization and scientific publications of foreign researchers on the influence of alcohol on the development of non-communicable diseases.Materials and research methods. Content analysis of documents of the World Health Organization and foreign studies on the assessment of the impact of alcohol In total, 48 documents of international organizations were analyzed, including the World Health Organization (WHO), the United Nations (UN), the International Agency for Research on Cancer (IARC-IARC) and the World Bank, as well as 211 scientific publications. Of these, 19 documents were selected, which included materials from WHO, UN, IARC and the World Bank on the impact of alcohol abuse on health, and 63 scientific publications on this topic. Selection criterion: the content in the documents of materials that adequately describe the impact of alcohol abuse on health as a risk factor for the development of various diseases.Results. An analysis of the documents and publications presented revealed a large massif of evidence that the harmful use of alcohol is a causal factor in the development of a number of noncommunicable diseases, an increase in mortality and disability at earlier stages of life, and the development of a link between harmful use of alcohol and a number of mental and behavioral disorders.
Structure and Assessment of the Oil Potential of Carbonaceous Carbonate-Siliceous Domanik Deposits in the Volga–Ural Oil and Gas Province
Authors: Fortunatova N.K. , Varlamov A.I. , Kanev A.S. , Poroskun V.I. , Baranova A.V. , Bushueva M.A. 
Published: August 2021

Abstract
—The paper considers the regularities in the structure and conditions of formation of Domanik carbonaceous carbonate-siliceous productive deposits. They are shown to occur in the stratigraphic interval from middle Frasnian to upper Famennian. The highly persistent structure of their sections for many kilometers within specific sedimentation zones and the drastic changes in the structure at the boundaries of the zones are justified. Lithological classification of rocks is considered. Methods and results of combined paleontological, lithological, geochemical, and petrophysical core studies are presented, as well as interpretation of well logging data and seismic-survey materials used in the assessment of oil resources and identification of oil- and gas-promising zones and areas. The conducted studies have proved significant oil resources in the Domanik productive sequence; their extraction might compensate for the decline in oil production from conventional pools.
Survive, Revive, Thrive: Chapter 10—Anchors Aweigh
Authors: Blasingame Tom 
Published: August 2021

It Is Time To Leave Port
Education is not a way to escape poverty; it is a way of fighting it.—Julius Nyerere, Tanzanian president, 1922–1999
As the COVID-19 pandemic subsides in most parts of the world, and as a global society we commit ourselves to its control and eradication everywhere, it is time for our “ship” to leave port. As we pull up our anchor (“anchors aweigh” means the anchor is off bottom and the ship is free to move), we must accept that there are risks out there, but we must get back to the task of exploration and production of oil and gas as never before. As I predicted in this column many months ago, we are definitely leaner (fewer people, with even more work to do) and now we need to be much meaner (better skilled, better motivated, and better focused). All the old adages apply: “life isn’t fair,” “there are no guarantees,” etc.—but a commitment to “duty, honor, and service” (an unofficial motto of my employer, Texas A&M University) stands firm in my mind for our industry. As we leave port, we must have the confidence and purpose that has defined our industry since its inception—improving lives, mitigating poverty, and providing the energy to enable a modern global society.

Reasons We Must Change as an Industry
Life’s a bit like mountaineering—never look down.— Edmund Hillary, New Zealand explorer, 1919–2008
I was in a panel session a few weeks back and, as SPE President, I am certain they saved the toughest question for me: “What are the reasons we must change as an industry?” I confess that this question was particularly hard because it requires a sketch of our future strategies as an industry and as a professional society, which in many ways remains undefined. Fortunately, I had some advance notice and was able to put some thought into my answer. Paraphrasing Darwin, “we must adapt or die.” It is that simple. Our industry provides enormous societal benefit, and just as the future of renewables lies in metals for batteries, conducting materials, circuitry, etc., the present and future of manufacturing lies in oil and gas. There simply are no viable substitutes.
Clinical Efficacy and Imaging Analysis for the Surgical Treatment of Thoracolumbar Infections in Elderly Patients
Authors: Zhong Yanlong , Tang Benyu , Lai Qi , Sheng Yonghong , Li Chao , Guo Jia , Luo Shiwei , Yao Haoqun , Wan Zongmiao 
Published: August 2021

Abstract
BackgroundFew reports have been conducted to comparing surgical results and safety evaluations between the different types of infections in geriatric patients. The aim of this study is to investigate the safety and efficacy of surgical treatment for thoracolumbar infections in elderly patients.Methods21 patients with pyogenic spondylodiscitis (PS) and 26 patients with tuberculous spondylodiscitis (TS) were enrolled in the study. All patients were treated using one-stage posterior debridement, decompression, and pedicle screw fixation. Comparison of operative safety parameters between the two groups. Clinical efficacy was evaluated using visual analog scale (VAS) score, the American Spinal Injury Association (ASIA) grade, the short form (SF)-36 survey and Oswestry disability index (ODI) to determine patient quality of life pre- and post-operatively. Results Hospitalisation and intensive care unit duration in the PS group were significantly shorter than in the TS group (P<0.05). The total incidence of post-operative complications for both groups was 44.7%. More complications occurred in the TS group, but the difference was not significant. The VAS and SF-36 scores (physical component) were significantly better in the PS group six months post-operatively, and the SF-36 (mental component) scores were significantly better in the PS group at the one-year follow-up. Neurological status in both groups improved post-operatively, and 83% of the patients were satisfied with the results of their operation. Imaging results showed that bone graft fusion improved in both groups at six months, one year and at the final follow-up. ConclusionSurgical treatment can provide satisfactory clinical and imaging results for thoracolumbar infection in patients over 65 years old. Although the incidence of peri-operative complications in the elderly is high, it is controllable and surgical treatment remains a safe and effective measure.
Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye Secale cereale L.)
Authors: Vendelbo Nikolaj , Mahmood Khalid , Sarup Pernille , Kristensen Peter , Orabi Jihad , Jahoor Ahmed 
Published: August 2021

Abstract
Efficient and stable restoration of male fertility (Rf) is a prerequisite for large-scale hybrid seed production but remains an inherent issue in the predominant fertility control system of rye (Secale cereale L.). The ‘Gülzow’ (G)-type cytoplasmic male sterility (CMS) system in hybrid rye breeding exhibits a superior Rf. While having received little scientific attention, one major G-type Rf gene has been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosomes. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using recent advents in rye genomic resources. This includes: (I) genome-wide association studies (GWAS) on G-type germplasm; (II) GWAS on a biparental mapping population; and (III) an RNA sequence study to investigate the expression of genes residing in Rf-associated regions in G-type rye hybrids. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome belonging to the mitochondrial transcription termination factor gene family. We provisionally denote the identified novel Rf gene on 3RL RfNOS1. The discovery made in this study is distinct from known P- and C-type systems in rye as well as recognized CMS systems in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). We believe this study constitutes a stepping stone towards understanding the restoration of male fertility in the G-type CMS system and potential resources for addressing the inherent issues of the P-type system.
Ecological impact evaluation of urban heat island in Dhaka city; a spatio-temporal approach
Authors: Rashid Kazi Jihadur , Islam Sumaia , Rahman Mohammad Atiqur 
Published: August 2021

Abstract
Urban heat island (UHI) is one of the major causes for deteriorating ecology of the rapidly expanding Dhaka city in the changing climatic conditions. Although researchers have identified, characterized and modeled UHI in the study area, the ecological evaluation of UHI effect has not yet been focused. This study uses land surface normalization techniques such as urban thermal field variance (UTFVI) to quantify the impact of UHI and also identifies vulnerable UHI areas compared to land cover types. Landsat imageries from 1990 to 2020 were used at decadal intervals. Results of the study primarily show that intensified UHI areas have increased spatially from 33.1–40.9% in response to urban growth throughout the period of 1990 to 2020. Extreme surface temperature values above 31°C have also been shown in open soils in under-construction sites for future developmental purposes. UTFVI is categorized into six categories representing UHI intensity in relation to ecological conditions. Finally, comparative analysis between land use/land cover (LULC) with UTFVI shows that the ecological conditions deteriorate as the intensity of UHI increases in the area. The developed areas facing ecological threat have increased from 9.3–19.8% throughout the period. Effective mitigating measures such as increasing green surfaces and planned urbanization practices are crucial in this regard. This study would help policymakers to concentrate on controlling thermal exposure and on preserving sustainable urban life.
No Evidence for an Association Between Variability in Sulcal Pattern and Academic Achievement
Authors: Roell Margot , Bellon E. , Polspoel B. , Declercq M. , De Smedt B. 
Published: August 2021

Abstract
Investigating how the brain may constrain academic achievement is not only relevant to understanding brain structure but also to providing insight into the origins of individual differences in these academic abilities. In this pre-registered study, we investigated whether the variability of sulcal patterns, a qualitative feature of the brain determined in-utero and not affected by brain maturation and learning, accounted for individual differences in reading and mathematics. Participants were 97 typically developing 10-year-olds. We examined (a) the association between the sulcal pattern of the intraparietal sulcus (IPS) and mathematical ability; (b) the association between the sulcal pattern of the occipitotemporal sulcus (OTS) and reading ability; and (c) the overlap and specificity of sulcal morphology of IPS and OTS and their associations with mathematics and reading. Despite its large sample, the present study was unable to replicate a previously observed relationship between the IPS sulcal pattern and mathematical ability and a previously observed association between the Left posterior OTS sulcal pattern and reading. We found no evidence for a possible overlap or specificity in the effect of sulcal morphology on mathematics and reading. Possible explanations for this absence of an association between sulcal morphology and academic achievement and suggestions for future research are discussed.