bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network Pharmacology and Bioinformatics Analysis on the Underlying Mechanisms of Matrine Against Uterine Corpus Endometrial Carcinoma
Authors: Cheng Sijin , Xu Yanan , Lei Feiyang , Yang Mingrui , Ma Cuiyan 
Published: December 2024

Background: Uterine corpus endometrial carcinoma (UCEC) ranks as a predominant tumor within the female reproductive system. Matrine, the chief active constituent of Compound Kushen Injection, exhibits antibacterial, antiviral, anti‐inflammatory, and antitumor effects. On the basis of previous research studies, this study utilized bioinformatics and network pharmacology approaches to investigate matrine’s anti‐UCEC potential, establishing a theoretical groundwork for subsequent mechanistic studies.Objective: Differentially expressed genes (DEGs) from the TCGA‐UCEC database were processed using R packages. Potential targets of matrine were identified via PubChem, SwissTargetPrediction, and BindingDB. The hub genes were identified through cytoHubba from the overlapping genes of matrine targets and UCEC DEGs. These genes were then subjected to cancer immune infiltration analysis, assessed with the R package “ggbeeswarm,” and validated by molecular docking studies.Results: We identified 28 potential drug–disease interaction genes and narrowed down to 10 hub genes. Notably, CDH1 and EPCAM were significantly differentially expressed between tumor and normal samples. Their expression levels correlated positively with multiple immune cells types. Molecular docking revealed significant interactions between CDH1, EPCAM, and matrine.Conclusions: These findings suggest that matrine’s anti‐UCEC activity may involve interactions with CDH1 and EPCAM. Advancing UCEC therapy based on these interactions could improve tumor diagnosis and treatment, including for UCEC.
Blocking Feedback Immunosuppression of Antigen Presentation in Brain Tumor During Oncolytic Virotherapy with oHSV-mshPKR
Authors: Tsuboi Nobushige , Rivera-Caraballo Kimberly A. , Sahu Upasana , Pacholczyk Rafal , Douglass Eugene , Johnson Theodore S. , Wang Qin , Kolhe Ravindra , Hedrick Catherine C. , Munn David H. , Hong Bangxing 
Published: December 2024

Abstract
Glioblastoma (GBM) is the most frequent malignant brain tumor. We recently discovered that oncolytic herpes simplex virus engineered to disable tumor-intrinsic protein kinase R (PKR) signaling (oHSV-shPKR) could increase oHSV oncolysis and antitumor immune response. However, in this study, we show that disabling tumor-intrinsic PKR signaling can also induce the activation of the indoleamine 2,3-dioxygenase (IDO) signaling pathway. Both GBM tumor progression and oHSV intratumoral therapy increased infiltration of IDO+CD11c+ dendritic cells (DC) into the tumor. The coculture of oHSV-infected human GBM neurospheres with monocyte-derived DCs (MoDC) dramatically increased IDO signaling activation in MoDCs through type-I IFN signaling. Addition of IDO inhibitor (indoximod) in the coculture significantly increased MoDC activation and reduced the consumption of tryptophan. Combining indoximod and oHSV significantly inhibited tumor growth and induced antigen-specific CD8+ T-cell activation. These results suggest that inhibition of the IDO pathway could significantly block feedback immunosuppression during oncolytic virotherapy of GBM.
Role of Infiltration on Land–Atmosphere Feedbacks in Central Europe: Fully Coupled WRF-Hydro Simulations Evaluated with Cosmic-Ray Neutron Soil Moisture Measurements
Authors: Arnault Joël , Fersch Benjamin , Schrön Martin , Bogena Heye Reemt , Hendricks Franssen Harrie-Jan , Kunstmann Harald 
Published: December 2024

Abstract
The skill of regional climate models partly relies on their ability to represent land–atmosphere feedbacks in a realistic manner, through coupling with a land surface model. However, these models often suffer from insufficient or erroneous information on soil hydraulic parameters. In this study, the fully coupled land–atmosphere model WRF-Hydro driven with ERA5 reanalysis is employed to reproduce the regional climate over central Europe with a horizontal resolution of 4 km for the period 2017–20. Simulated soil moisture is compared with data from cosmic-ray neutron sensors (CRNSs) at three terrestrial environmental observatories. Soil hydraulic parameters from continental and global digital soil datasets (SoilGrids and EU-SoilHydroGrids), together with Campbell and van Genuchten–Mualem retention curve equations, are used to assess the role of infiltration on modeled land–atmosphere feedbacks. The percolation parameter is calibrated to better capture observed discharge amounts in the observatories. WRF-Hydro with Campbell and SoilGrids gives the lowest mean annual temperature and mean annual precipitation differences compared to the E-OBS product from European Climate Assessment and Dataset, which is achieved by reducing soil moisture in the rootzone, increasing air temperature, and decreasing precipitation through a positive soil moisture–precipitation feedback process. WRF-Hydro with van Genuchten–Mualem and EU-SoilHydroGrids best reproduces CRNS soil moisture daily variations, despite enhanced positive biases that generate a larger proportion of convective precipitation favored over wet soils and spurious discharge peaks. This study demonstrates the importance of infiltration processes to realistically reproduce land–atmosphere feedbacks.

Significance Statement
The ability to correctly reproduce the feedbacks between land and atmosphere is crucial for a regional climate model. This study shows that the nature of these land–atmosphere feedbacks is partly related to the description of soil infiltration, which potentially helps to improve regional climate modeling results. An updated method to disentangle the proportion of convective precipitation being favored over wet, dry, and mixed soils is provided, which sheds new light on the soil moisture–precipitation feedback mechanism.
Plasma acute phase proteins as potential predictors of intra-amniotic inflammation and infection in preterm premature rupture of membranes
Authors: Cho Hee Young , Park Kyo Hoon , Oh Eunji , Lee Min Jung , Choi Bo Young , Im Eun Mi 
Published: December 2024

Background We aimed to investigate the potential of altered levels of various acute phase proteins (APPs) in the plasma, either used alone or in combination with ultrasound-, clinical-, and conventional blood-based tests, for predicting the risk of intra-amniotic inflammation (IAI), microbial invasion of the amniotic cavity (MIAC), histologic chorioamnionitis (HCA), and funisitis in women with preterm premature rupture of membranes (PPROM). Methods A total of 195 consecutive pregnancies involving singleton women with PPROM (at 23 + 0–34 + 0 weeks) who underwent amniocentesis and from whom plasma samples were obtained at amniocentesis were retrospectively included in this study. Amniotic fluid (AF) was cultured to assess the MIAC and analyzed for interleukin (IL)-6 levels to define IAI (AF IL-6 level of ≥2.6 ng/mL). The plasma concentrations of hepcidin, mannose-binding lectin (MBL), pentraxin-2, retinol-binding protein 4 (RBP4), serum amyloid A1 (SAA1), and serpin A1 were determined using ELISA. Ultrasonographic cervical length (CL), neutrophil-to-lymphocyte ratio (NLR), and C-reactive protein levels were measured. IAI/MIAC was defined as IAI, MIAC, or both. Results Multivariate logistic regression analyses showed the following: (1) elevated plasma levels of hepcidin and SAA1 and decreased levels of RBP4 in the plasma were independently associated with IAI/MIAC and (2) decreased plasma RBP4 levels were independently associated with funisitis; however, (3) none of the plasma APPs investigated were associated with acute HCA when adjusted for baseline covariates. Using stepwise regression analysis, noninvasive prediction models comprising plasma RBP4 levels, CL, NLR, and gestational age at sampling were proposed, which provided a good prediction of IAI/MIAC and funisitis (area under the curve: 0.80 and 0.72, respectively). Conclusions Hepcidin, RBP4, and SAA1 were identified as potential APP biomarkers in the plasma predictive of IAI/MIAC or funisitis in patients with PPROM. In particular, combination of these APP biomarkers with ultrasound-, clinical-, and conventional blood-based markers can significantly support the diagnosis of IAI/MIAC and funisitis.
Exploring the relationships between self-efficacy, self-care, and glycaemic control in primary care diabetes management
Authors: Quynh Anh Le Ho Thi , Quoc Huy Nguyen Vu , Minh Tam Nguyen , Wens Johan , Derese Anselme , Peersman Wim , Ha My Vo Ngoc , Thang Tran Binh , Phuong Anh Nguyen Thi , Truc Ly Tran Thi , Pype Peter 
Published: December 2024

Objectives: Our study aimed to identify the complex interplay between self-efficacy, self-care practice, and glycaemic control among people with type 2 diabetes mellitus (PWDs) to inform the design of more targeted and effective behavioural interventions in primary care. Methods: A cross-sectional descriptive study was performed with 294 PWDs managed in primary care. The Diabetes Management Self-Efficacy Scale (DMSES) and Summary of Diabetes Self-Care Activities (SDSCA) questionnaire measured patients’ self-efficacy and self-care practice. Multivariate logistic regression models were developed to explore how SDSCA, DMSES, and their combined effect relate to glycaemic control, adjusting for patient characteristics. Network analysis in R software examined relationships between self-efficacy and self-care dimensions across glycaemic control subgroups using a Gaussian graphical model with the extended Bayesian information criterion. Results: Half the PWDs (50.7%) had suboptimal glycaemic control. Better glycaemic control was consistently associated with higher self-efficacy (odds ratio (OR) = 0.76, 95% confidence (CI) (0.60, 0.97), p = 0.03), shorter duration of diabetes (OR = 1.89, 95% CI (1.08, 3.31), p = 0.03), normal waist circumference (OR = 1.76, 95% CI (1.02, 3.05), p = 0.04), absence of diabetes complications (OR = 2.09, 95% CI (1.10, 3.98), p = 0.02), and treatment with oral hypoglycaemic agents (OR = 3.05, 95% CI (1.53, 6.09), p < 0.01). Network analysis among people with well-controlled HbA1c revealed that diet adherence and self-efficacy had the most robust connection, with diet self-efficacy strongly associated with most self-efficacy dimensions. Self-efficacy in exercise, blood glucose monitoring, and foot care are the most central factors in the network structures for PWDs with suboptimal glycaemic control. Conclusions: Our study highlights the critical role of self-efficacy in diabetes primary care. For people with optimal glycaemic control, prioritising self-efficacy in diet adherence is crucial for sustaining glycaemic outcomes and supporting other self-care behaviours. Among those with suboptimal glycaemic control, enhancing self-efficacy in exercise, blood glucose monitoring, and foot care is essential. Targeted education programs, personalised counselling, and E-health tools can further empower patients to manage their diabetes more effectively.
Occupational Health Injuries and Illness Among Women Workers in the Chemical Industry: A Scoping Review
Authors: Romero-Serrano Rocío , Arnaiz Carmen , Torres-Enamorado Dolores , Lancharro-Tavero Inmaculada , Arroyo-Rodríguez Almudena 
Published: December 2024

Background: The number of women in the chemical industry has recently increased due to more women pursuing science careers. It is necessary, therefore, to analyze the emerging health risks for female workers in the chemical industry. This study examines the relationship between occupational health and sex/gender in the chemical industry, with a gender perspective. Methods: We present a scoping review ( n = 97). After removing duplicates and applying eligibility criteria, we selected 27 articles published in the last decade that explored the industry’s occupational risks. Findings: Most of the papers include predominantly male samples and describe adult populations, mainly from developed countries. The studies focus on various employment contexts of chemical industries. We identified health risks in oncology, dermatology, and the respiratory system, among others. We found that particular emphasis was given to the relationship between occupational exposure and cancer, especially breast cancer. Furthermore, we observed sex/gender differences in the prevalence of respiratory and dermatological disorders. These results highlight the need to consider specific sex/gender-based health risk factors in the chemical industry. Conclusions/Application to Practice: The chemical industry is considered a crucial health determinant, however, the studies focused on sex/gender-based differences without considering gender-specific physiology and work circumstances. Although some studies do mention sex/gender disparities, such as occupational rhinitis, which is more frequent in women, studies are scarce. The absence of a segregated analysis with a gender perspective could lead to the ignorance of emerging health risks for female workers, highlighting the urgent need to include a gender perspective in future research.
Surgical Correction of Thoracic and Thoracolumbar TB Kyphosis by Posterior Only Approach Using Rajasekaran’s Kyphosis Classification
Authors: Kalanjiyam Guna Pratheep , Ramachandran Karthik , Rajasekaran Shanmuganathan , Shetty Ajoy Prasad , Kanna Rishi Mugesh 
Published: December 2024

Study design Prospective analysis of retrospective data. Objectives To analyse the clinical and radiological outcomes of thoracic and thoracolumbar TB kyphosis by a posterior-only approach using kyphosis classification. Methods Patients with thoracic and thoracolumbar spinal TB who underwent posterior-only surgical correction for kyphotic deformity >30° were categorized into Group: A (Active TB) and Group B (Healed TB). Demographic, clinical and radiological data were collected, and kyphotic deformity was classified according to Rajasekaran classification. Results 52 patients with a minimum 2-year follow-up were included. Group A included 25 patients with mean preop kyphosis of 39.5° (31.2°-53.7°), and Group B included 27 patients with mean preop kyphosis of 85° (44.2°-125.2°). Among 25 patients in Group A, 19 were Type IIIA, and 6 were Type IIA kyphosis. In Group B, 18 patients were Type IIIB, 5 were Type III C, 3 were Type IIA, and 1 was IIIA kyphosis. All patients in group A underwent posterior column shortening by Smith-Peterson Osteotomy, with 14 patients requiring additional anterior column reconstruction. Patients in Group B required Closing-Opening Wedge Osteotomy (18), Halo followed by vertebral column resection (6), disc bone osteotomy (2) and pedicle subtraction osteotomy (1). Mean kyphosis at 2 years follow-up was 20.8° (11.2°-32.8°) in Group A and 53.5° (8.1°- 96.4°) in Group B. Conclusions Correction of kyphosis in spinal TB involves completely different strategies in active and healed disease. Hence, a posterior-only treatment approach using Rajasekaran’s kyphosis classification can help surgeons in appropriate decision-making.
Postacute Myocardial Infarction Differences in Physical Activity Behavior, Anxiety, and Depression Levels
Authors: Shajrawi Abedalmajeed Methqal , Al-Akash Hekmat Yousef , Al-Smadi Ahmed Mohammad , Masa'deh Rami , Aburuz Mohannad Eid , Khalil Heba , Hweidi Issa Moh'd , Saifan Ahmad Rajeh 
Published: December 2024

Background There are limited studies regarding physical activity, anxiety, and depression levels among patients with postacute myocardial infarction (AMI). This is a need for more research on physical activity, anxiety, and depression levels based on the type of AMI following hospitalization to develop interventions to improve these outcomes. Objective To compare physical activity behavior, anxiety, and depression levels among patients with post-ST-elevation myocardial infarction (STEMI) and post-non-ST-elevation myocardial infarction (NSTEMI) not involved in cardiac rehabilitation program following hospitalization. Methods A descriptive cross-sectional study design was employed with a convenience sample of 254 patients with post-AMI, 4 weeks after hospitalization. Participants, recruited from three hospitals in Jordan. Results The sample characteristics included the following: 140 males (54.3%), 120 married (47.2%), diagnosed with STEMI (n = 137, 53.1%) or NSTEMI (n = 117, 45.3%). And 41.3% of participants had moderate-to-severe anxiety level (General Anxiety Disorder-7 [GAD-7] > 10), while 22.0% had moderate to severe depression levels (Patient Health Questionnaire-9 [PHQ-9] > 10). Post-STEMI and post-NSTEMI participants had moderate levels of anxiety, with 14.39 (3.45) versus 13.37 (3.51); and depression levels of 12.66 (3.28) versus 12.22 (3.54), respectively. There was no significant difference in depression level between patients with post-STEMI and post-NSTEMI, but the former had statistically significant higher anxiety level than the latter. The mean self-reported exercise duration and exercise frequency of patients with post-STEMI were significantly higher than for their post-NSTEMI counterparts: 151.7 (76.33) versus 87.78 (37.62) minutes per week, and 2.92 (1.44) versus 1.97 (0.88) times per week (respectively). Furthermore, patients with post-STEMI had less mean sedentary time: 582.92 (128.92) and 641.54 (147. 27) minutes per day ( p > .05) (respectively). Conclusions Healthcare providers have to consider the differences in physical activity behavior, anxiety, depression levels based on types of AMI when developing interventions and establishing cardiac rehabilitation program to improve physical activity behavior and reducing sedentary time.
Axial Rotation Analysis in Total Ankle Arthroplasty Using Weight-Bearing Computer Tomography and Three-Dimensional Modeling
Authors: Barbero Agustin , Efrima Ben , Ovadia Joshua , Indino Cristian , Maccario Camilla , Giuseppe Usuelli Federico 
Published: December 2024

Category: Ankle Arthritis; Other Introduction/Purpose: Post-operative alignment is the most critical indicator for a successful total ankle arthroplasty (TAA). Total ankle malrotation is associated with an increased risk for polyethylene wear and medial gutter pain. Currently, there is no consensus on the correct way to measure the alignment of the tibial and talar component rotations in the axial plane. In the current study, the post-operative analysis system was assessed using weight-bearing computer tomography and a three-dimensional (3D) model. The purpose of the study was to assess the inter-observer and intra-observer agreement of this system. Methods: Four angles were measured by two raters independently in two separate readings: posterior tibial component rotation angle (PTIRA), posterior talar component rotation angle (PTARA), tibia talar component axial angle (TTAM), and tibial component to the second metatarsal angle (TMRA). Agreement analysis was quantified according to the interclass coefficient Results: Sixty TAAs across 60 patients were evaluated. A good inter-observer agreement and intra-observer agreement when measuring the PTIRA, PTARA, and TTAM angles was observed along with an excellent inter-observer agreement and intra-observer agreement when measuring the TMRA angle. Conclusion: In conclusion, the current 3D model-based measurement system demonstrates good to excellent inter and intra-agreement. According to these results, 3D modelling can be reliably used to measure and assess the axial rotation of TAA components. Figure 3: Example case of how to measure the angles for assessing axial rotation of TAA and forefoot. 3 A) Posterior TIbial component Rotational Angle (PTIRA). The chosen slice is the one with the straightest posterior wall component of the tibia. The angle is measured on the following steps. First a reference is put on the posterior wall of the tibial component (blue horizontal line). Then the angle is measured starting from the center of the tibial axis (red dot explained on fig. 2), going to the fibular axis (medial part of the red line at the right of the draw), and finally ending 90° to the posterior reference line. 3 B) Posterior TAlar component Rotational Angle (PTARA). The chosen slice is the one with the straightest posterior wall component of the talus. For the angle measurement. The same steps as figure 3 A are followed with the talar component. 3 C) Talar component to 2nd Metatarsal Rotational Angle (TMRA): Defined as an intersection between a line parallel to the lateral border of the tibia component and a line perpendicular to the anatomical axis of the second metatarsal. This was measured using a 3D rendering tool
Bilateral External Torque CT Reliably Detects Syndesmotic Lesions in an Experimental Cadaveric Study
Authors: Beeler Silvan , Ongini Esteban , Hochreiter Bettina , Sutter Reto , Viehoefer Arnd , Wirth Stephan H. , Calek Anna-Katharina 
Published: December 2024

Category: Sports; Trauma Introduction/Purpose: If tibiofibular syndesmosis injury is missed, chronic instability may lead to persistent pain and osteoarthritis. So far, no reliable diagnostic method has been available. The primary objectives of this study were to determine whether defined lesions of the syndesmosis can be correlated with specific tibiofibular joint displacements caused by external rotation (ER) torques and to compare its performance with arthroscopy. Secondary objectives included evaluation of Intraclass-Correlation-Coefficients (ICCs) and suitability of the healthy contralateral ankle as a reference. Methods: Seven pairs of healthy lower legs were tested and assigned to two groups (1) Supination-ER (SER) and (2) Pronation-ER (PER). In the intact state and after each surgical step, an ankle arthroscopy and three CT scans were performed. During the scans, the specimens were placed in an external torque device with 2.5Nm, 5Nm and 7.5Nm. Results: The arthroscopic and radiological parameters showed significant correlations in all pairwise comparisons. The receiver operating curve (ROC) analyses yielded best prediction of syndesmotic instability with the anterior tibiofibular distance (AD) on CT with a sensitivity of 84.1% and a specificity of 95.2% (area under the curve (AUC) 94.8%, CI 0.916-0.979, p< 0.0001) and middle tibiofibular distance at arthroscopy with a sensitivity of 76.2% and specificity of 92.3% (AUC 91.2%, CI 0.837-0.987, p< 0.0001). Higher torques increased the rate of true positive results. Conclusion: Bilateral external torque CT reliably detects experimental syndesmotic rotational instability compared to the healthy side with greater sensitivity and similar specificity to the arthroscopic lateral hook test. Translation of these experimental findings to clinical practice remains to be established.