bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical impact of implementing humidified high-flow nasal cannula on interhospital transport among children admitted to a PICU with respiratory distress: a cohort study
Authors: Miura Shinya , Yamaoka Kazue , Miyata Satoshi , Butt Warwick , Smith Sile 
Published: June 2021

Abstract
Background
There is a limited evidence for humidified high-flow nasal cannula (HHFNC) use on inter-hospital transport. Despite this, its use during transport is increasing in children with respiratory distress worldwide. In 2015 HHFNC was implemented on a specialized pediatric retrieval team serving for Victoria. The aim of this study is to investigate the effect of the HHFNC implementation on the retrieval team on the paediatric intensive care unit (PICU) length of stay and respiratory support use.

Methods
We performed a cohort study using a comparative interrupted time-series approach controlling for patient and temporal covariates, and population-adjusted analysis. We studied 3022 children admitted to a PICU in Victoria with respiratory distress January 2010–December 2019. Patients were divided in pre-intervention era (2010–2014) and post-intervention era (2015–2019).

Results
1006 children following interhospital transport and 2016 non-transport children were included. Median (IQR) age was 1.4 (0.7–4.5) years. Pneumonia (39.1%) and bronchiolitis (34.3%) were common. On retrieval, HHFNC was used in 5.0% (21/420) and 45.9% (269/586) in pre- and post-intervention era. In an unadjusted model, median (IQR) PICU length of stay was 2.2 (1.1–4.2) and 1.7 (0.9–3.2) days in the pre- and post-intervention era in transported children while the figures were 2.4 (1.3–4.9) and 2.1 (1.2–4.5) days in non-transport children. In the multivariable regression model, the intervention was associated with the reduced PICU length of stay (ratio 0.64, 95% confidential interval 0.49–0.83, p = 0.001) with the predicted reduction of PICU length of stay being − 10.6 h (95% confidential interval − 16.9 to − 4.3 h), and decreased respiratory support use (− 25.1 h, 95% confidential interval − 47.9 to − 2.3 h, p = 0.03). Sensitivity analyses including a model excluding less severe children showed similar results. In population-adjusted analyses, respiratory support use decreased from 4837 to 3477 person-hour per year in transported children over the study era, while the reduction was 594 (from 9553 to 8959) person-hour per year in non-transport children. With regard to the safety, there were no escalations of respiratory support mode during interhospital transport.

Conclusions
The implementation of HHFNC on interhospital transport was associated with the reduced PICU length of stay and respiratory support use among PICU admissions with respiratory distress.
Destination shapes antibiotic resistance gene acquisitions, abundance increases, and diversity changes in Dutch travelers
Authors: D’Souza Alaric W. , Boolchandani Manish , Patel Sanket , Galazzo Gianluca , van Hattem Jarne M. , Arcilla Maris S. , Melles Damian C. , de Jong Menno D. , Schultsz Constance ,   COMBAT Consortium, Bootsma Martin C. J. , van Genderen Perry J. , Goorhuis Abraham , Grobusch Martin P. , Molhoek Nicky , Oude Lashof Astrid M. L. , Stobberingh Ellen E. , Verbrugh Henri A. , Dantas Gautam , Penders John 
Published: June 2021

AbstractBackgroundAntimicrobial-resistant bacteria and their antimicrobial resistance (AMR) genes can spread by hitchhiking in human guts. International travel can exacerbate this public health threat when travelers acquire AMR genes endemic to their destinations and bring them back to their home countries. Prior studies have demonstrated travel-related acquisition of specific opportunistic pathogens and AMR genes, but the extent and magnitude of travel’s effects on the gut resistome remain largely unknown.MethodsUsing whole metagenomic shotgun sequencing, functional metagenomics, and Dirichlet multinomial mixture models, we investigated the abundance, diversity, function, resistome architecture, and context of AMR genes in the fecal microbiomes of 190 Dutch individuals, before and after travel to diverse international locations.ResultsTravel markedly increased the abundance and α-diversity of AMR genes in the travelers’ gut resistome, and we determined that 56 unique AMR genes showed significant acquisition following international travel. These acquisition events were biased towards AMR genes with efflux, inactivation, and target replacement resistance mechanisms. Travel-induced shaping of the gut resistome had distinct correlations with geographical destination, so individuals returning to The Netherlands from the same destination country were more likely to have similar resistome features. Finally, we identified and detailed specific acquisition events of high-risk, mobile genetic element-associated AMR genes includingqnrfluoroquinolone resistance genes,blaCTX-Mfamily extended-spectrum β-lactamases, and the plasmid-bornemcr-1colistin resistance gene.ConclusionsOur results show that travel shapes the architecture of the human gut resistome and results in AMR gene acquisition against a variety of antimicrobial drug classes. These broad acquisitions highlight the putative risks that international travel poses to public health by gut resistome perturbation and the global spread of locally endemic AMR genes.
Genome‑wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism
Authors: Luo Jingyi , Wang Xiaoxia , Yuan Li , Guo Lixin 
Published: June 2021

Abstract
Background
Type 2 diabetes mellitus (T2DM) and hypothyroidism are two common endocrine diseases and the phenomenon that the prevalence of diabetes-related hypothyroidism shows a significant upward trend deserves further attention, but the specific pathogenesis is not yet clear. The study aimed to explore the molecular mechanisms on DNA methylation regulating gene expression and participating in diabetes-related hypothyroidism through genome-wide DNA methylation and RNA sequencing.

Results
The prevalence of hypothyroidism in T2DM patients was significantly higher than that in patients without T2DM (P = 0.018). Meanwhile, high TSH and low T3 and T4 levels were detected in diabetic mice. Low T3 and T4 levels were detected in Nthy-ori3-1 cells incubated in high-glucose medium. Differentially expressed genes (DEGs) and differentially methylated regions (DMRs) were detected by RNA sequencing and reduced representation bisulfite sequencing in Nthy-ori3-1 cells cultured in high-glucose and normal medium. Functional enrichment analyses reveled that DMRs and DEGs were related to significant pathways including Ras, Wnt and MAPK pathways.

Conclusions
We observed the potential connection between T2DM and hypothyroidism. This study was the first one carrying out DNA methylation and gene expression profiles to explore epigenetic modification in diabetes-related hypothyroidism, which provided information for the detailed study of the molecular mechanism in diabetes-related hypothyroidism.
A certain q-Ruscheweyh type derivative operator and its applications involving multivalent functions
Authors: Khan Bilal , Srivastava H. M. , Arjika Sama , Khan Shahid , Khan Nazar , Ahmad Qazi Zahoor 
Published: June 2021

AbstractIn the present paper, by using the concept of convolution and q-calculus, we define a certain q-derivative (or q-difference) operator for analytic and multivalent (or p-valent) functions. This presumably new q-derivative operator is an extension of the known q-analogue of the Ruscheweyh derivative operator. We also give some interesting applications of this q-derivative operator for multivalent functions by using the method of differential subordination. Relevant connections with a number of earlier works on this subject are also pointed out.
Policy-relevant insights for regional renewable energy deployment
Authors: De Laurentis Carla , Pearson Peter J. G. 
Published: June 2021

Abstract
Background
The paper explores how regional actors engage with energy systems, flows and infrastructures in order to meet particular goals and offers a fine-tuned analysis of how differences arise, highlighting the policy-relevant insights that emerge.

Methods
Using a novel framework, the research performs a comparative case study analysis of three regions in Italy and two of the devolved territories of the UK, Wales and Scotland, drawing on interviews and documentary analysis.

Results
The paper shows that acknowledging the socio-materialities of renewable energy allows a fine-tuned analysis of how institutions, governance and infrastructure can enable/constrain energy transitions and policy effectiveness at local and regional levels. The heuristic adopted highlights (i) the institutions that matter for renewable energy and their varied effects on regional renewable energy deployment; (ii) the range of agencies involved in strategically establishing, contesting and reproducing institutions, expectations, visions and infrastructure as renewable energy deployment unfolds at the regional level and (iii) the nature and extent of infrastructure requirements for and constraints on renewable energy delivery and how they affect the regional capacity to shape infrastructure networks and facilitate renewable energy deployment. The paper shows how the regions investigated developed their institutional and governance capacity and made use of targets, energy visions and spatial planning to promote renewable energy deployment. It shows that several mediating factors emerge from examining the interactions between regional physical resource endowments and energy infrastructure renewal and expansion. The analysis leads to policy-relevant insights into what makes for renewable energy deployment.

Conclusion
The paper contributes to research that demonstrates the role of institutional variations and governance as foundations for geographical differences in the adoption of renewable energy, and carries significant implications for policy thinking and implementation. It shows why and how policy-makers need to be more effective in balancing the range of goals/interests for renewable energy deployment with the peculiarities and specificities of the regional contexts and their infrastructures. The insights presented help to explain how energy choices and outcomes are shaped in particular places, how differences arise and operate in practice, and how they need to be taken into account in policy design, policy-making and implementation.
Does continuity in nursing staff matter? A pilot study on correlation of central line-associated bloodstream infections and employee turnover
Authors: Scheier Thomas , Kuster Stefan P. , Dunic Mesida , Falk Christian , Sax Hugo , Schreiber Peter W. 
Published: June 2021

Abstract
Background
Understaffing has been previously reported as a risk factor for central line-associated bloodstream infections (CLABSI). No previous study addressed the question whether fluctuations in staffing have an impact on CLABSI incidence. We analyzed prospectively collected CLABSI surveillance data and data on employee turnover of health care workers (HCW) to address this research question.

Methods
In January 2016, a semiautomatic surveillance system for CLABSI was implemented at the University Hospital Zurich, a 940 bed tertiary care hospital in Switzerland. Monthly incidence rates (CLABSI/1000 catheter days) were calculated and correlations with human resources management-derived data on employee turnover of HCWs (defined as number of leaving HCWs per month divided by the number of employed HCWs) investigated.

Results
Over a period of 24 months, we detected on the hospital level a positive correlation of CLABSI incidence rates and turnover of nursing personnel (Spearman rank correlation, r = 0.467, P = 0.022). In more detailed analyses on the professional training of nursing personnel, a correlation of CLABSI incidence rates and licensed practical nurses (Spearman rank correlation, r = 0.26, P = 0.038) or registered nurses (r = 0.471, P = 0.021) was found. Physician turnover did not correlate with CLABSI incidence (Spearman rank correlation, r =  −0.058, P = 0.787).

Conclusions
Prospectively determined CLABSI incidence correlated positively with the degree of turnover of nurses overall and nurses with advanced training, but not with the turnover of physicians. Efforts to maintain continuity in nursing staff might be helpful for sustained reduction in CLABSI rates.
Post-translational regulation of autophagy is involved in intra-microbiome suppression of fungal pathogens
Authors: Wang Jing , Xu Chaoyun , Sun Qiming , Xu Jinrong , Chai Yunrong , Berg Gabriele , Cernava Tomislav , Ma Zhonghua , Chen Yun 
Published: June 2021

Abstract
Background
Microbiome interactions are important determinants for ecosystem functioning, stability, and health. In previous studies, it was often observed that bacteria suppress potentially pathogenic fungal species that are part of the same plant microbiota; however, the underlying microbe-microbe interplay remains mostly elusive. Here, we explored antagonistic interactions of the fungus Fusarium graminearum and bacterium Streptomyces hygroscopicus at the molecular level. Both are ubiquitous members of the healthy wheat microbiota; under dysbiosis, the fungus causes devastating diseases.

Results
In co-cultures, we found that Streptomyces alters the fungal acetylome leading to substantial induction of fungal autophagy. The bacterium secrets rapamycin to inactivate the target of rapamycin (TOR), which subsequently promotes the degradation of the fungal histone acetyltransferase Gcn5 through the 26S proteasome. Gcn5 negatively regulates fungal autophagy by acetylating the autophagy-related protein Atg8 at the lysine site K13 and blocking cellular relocalization of Atg8. Thus, degradation of Gcn5 triggered by rapamycin was found to reduce Atg8 acetylation, resulting in autophagy induction in F. graminearum.

Conclusions
Autophagy homeostasis plays an essential role in fungal growth and competition, as well as for virulence. Our work reveals a novel post-translational regulation of autophagy initiated by a bacterial antibiotic. Rapamycin was shown to be a powerful modulator of bacteria–fungi interactions with potential importance in explaining microbial homeostasis in healthy plant microbiomes. The autophagic process provides novel possibilities and targets to biologically control pathogens.
After the paint has dried: a review of testing techniques for studying the mechanical properties of artists’ paint
Authors: dePolo Gwen , Walton Marc , Keune Katrien , Shull Kenneth R. 
Published: June 2021

Abstract
While the chemistry of artists’ paints has previously been studied and reviewed, these studies only capture a portion of the properties affecting the response of paint materials. The mechanical properties of artists’ paints relate to the deformation response of these materials when a stress is applied. This response is dependent on many factors, such as paint composition, pigment to binder ratio, temperature, relative humidity, and solvent exposure. Here, thirty years of tensile testing data have been compiled into a single dataset, along with the testing conditions, to provide future researchers with easy access to these data as well some general discussion of their trends. Alongside the more commonly used techniques of tensile testing and dynamic mechanical analysis, new techniques have been developed to more fully investigate the mechanical properties, and are discussed along with salient results. The techniques have been divided into two categories: those that are restricted to use on model systems and those that are applicable to historic samples. Techniques applied to model systems (tensile testing, dynamic mechanic analysis, quartz crystal microbalance, vibration studies) require too large of a sample to be taken from art objects or focus on the mechanical properties of the liquid state (shear rheometry). Techniques applied to historic samples incorporate the use of small sample sizes (nanoindentation), optical techniques (laser shearography), computational simulations (finite element analysis), and non-invasive comparative mechanical properties (single-sided nuclear magnetic resonance) to investigate and predict the mechanical properties of paints.
Factors influencing the removal torque of palatal implant used for orthodontic anchorage
Authors: Schätzle Marc Andreas , Hersberger-Zurfluh Monika , Patcas Raphael 
Published: June 2021

Abstract
Background
A non-invasive method has recently been introduced to remove osseo-integrated palatal implants by using the implantation ratchet which is designed to screw in or unscrew the implants. Although a proof of concept has been published, the torque involved to successfully explant have not been investigated so far. The aim of this study was to assess the removal torque required to explant osseo-integrated and orthodontically utilized palatal implants, and to identify potentially influencing variables.

Materials and method
Thirty-one consecutive patients (15 females, 16 males; mean age 24.1 ±7.4 years) with fully osseo-integrated and previously orthodontically loaded palatal implants (Orthosystem®: diameter 4.1mm/length 4.2mm/sandblasted with large grits (SLA) surface) were randomly assigned to either clockwise or counter-clockwise non-invasive explantation. The respective explantation tool with an electric torque control was placed on the abutment connection of the implant and secured by an occlusal screw.
The primary outcome studied was maximal removal torque (MRT) needed to detach the implant from its socket which was recorded individually together with other potentially influencing secondary outcomes (gender, age, orthodontic loading time, use of local anaesthetics). Student’s t-test was used to contrast MRT difference for the gender, type of suprastructure, use of local anaesthetics, and rotational direction.
Spearman correlations was used to investigate associations between MRT and patient’s age or duration loading time.

Results
Average MRT (148.6 ± 63.2N/cm) using ratchet as a non-invasive removal method of palatal implant was considered safe. The triangular head fractured of palatal implant at a torque level of 300.1 Ncm. Significantly higher explantation were recorded for male patients compared to female patients (182.0 ± 63.0 Ncm vs 112.8 ± 40.8 Ncm; P=0.001). On the other side, the mean removal torque for palatal removal in clockwise direction was non-significantly different (158.3 ± 58.6 Ncm) compared to counter-clockwise direction (139.4 ± 67.9 Ncm). Neither patient’s age (p=0.324) nor loading time (p=0.214) were significantly correlated with removal torque values.

Conclusions
Pertinent literature on this subject is practically non-existent, as orthodontics is presumably the only discipline where implant removal represents a treatment success. Mean MRT for successful palatal implant removal was 148.6±63.2Ncm, but a large spectrum was observed (minimum 31.5Ncm, maximum 272.8Ncm). This obvious heterogeneity underlines the importance to investigate possible influencing factors. The safe and simple non-invasive method for palatal implant removal necessitates moderate, but not high torque MRTs, independently of the torque direction. The necessary MRT seems clearly influenced by gender, but less so by patient’s age or loading time.
Classification of SSVEP-based BCIs using Genetic Algorithm
Authors: Soltani Hamideh , Einalou Zahra , Dadgostar Mehrdad , Maghooli Keivan 
Published: June 2021

AbstractBrain computer interface (BCI) systems have been regarded as a new way of communication for humans. In this research, common methods such as wavelet transform are applied in order to extract features. However, genetic algorithm (GA), as an evolutionary method, is used to select features. Finally, classification was done using the two approaches support vector machine (SVM) and Bayesian method. Five features were selected and the accuracy of Bayesian classification was measured to be 80% with dimension reduction. Ultimately, the classification accuracy reached 90.4% using SVM classifier. The results of the study indicate a better feature selection and the effective dimension reduction of these features, as well as a higher percentage of classification accuracy in comparison with other studies.
Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects
Authors: Feher Balazs , Apaza Alccayhuaman Karol Ali , Strauss Franz Josef , Lee Jung-Seok , Tangl Stefan , Kuchler Ulrike , Gruber Reinhard 
Published: June 2021

Abstract
Background
Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However, evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of bilayer collagen membranes has an effect on bone regeneration.

Methods
In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter, the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter) were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area.

Results
Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3, 4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upside-down and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8 mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial distribution of bone with and without collagen fibers, as well as residual collagen fibers.

Conclusions
Our data support the osteoconductive properties of collagen membranes and suggest that bone regeneration is facilitated regardless of membrane layer alignment.