bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Insight into Anticancer Bioactives from Punica granatum (Pomegranate)
Authors: Sharma Kanika , Kesharwani Payal , Prajapati Shiv Kumar , Jain Ankit , Jain Dolly , Mody Nishi , Sharma Swapnil 
Published: July 2021

:Cancer is one of the major reasons for mortality across the globe. Many side-effects are associated with the formulations available in the market, affecting the quality of life of the patients. This has caused the researchers to find an alternative source of medications, such as herbal medicine, showing a promising effect in anticancer treatment; one such source is Pomegranate, which belongs to the family Punicaceae. Punica granatum contains many polyphenols that have antioxidant, antidiabetic, and therapeutic effects in the treatment and management of metabolic and cardiovascular diseases, as well as a favourable effect on anticancer therapy. Polyphenols like punicalin, punicalagin, and ellagic acid are a few of the many compounds responsible for the anticancer activity of pomegranate. Many preparations of pomegranate, such as Pomegranate Juice (PJ), Pomegranate seed oil (PSO), Pomegranate peel extract (PoPx), etc. are used in various clinical studies. These polyphenols show anticancer activity by either arresting the cell cycle in the G2/M phase, inducing apoptosis or damaging the DNA of tumor cells. This review explicitly discusses the role and mechanism of bioactives obtained from the pomegranate in the treatment and management of cancer. The chemical structure, properties, and role of pomegranate in the treatment of breast, lung, thyroid, colon, and prostate cancer have been focused on in detail. This review also discusses various targeted drug delivery approaches for tumour treatment as well as patented preparation of pomegranate compounds along with the ongoing clinical trials.
The Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine Treatment in Resistant 2D
and 3D Model Triple Negative Breast Cancer Cell Line: ABCG2 Expression Data
Authors: Ata Fatma Kubra , Yalcin Serap 
Published: July 2021

Background:
Chemotherapeutics have been commonly used in cancer treatment.

Objective:
In this study, the effects of Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine have been evaluated on
two-dimensional (2D) (sensitive and resistance) cell lines and three dimensional (3D) spheroid structure of MDA-MB-
231. The 2D cell culture lacks a natural tissue-like structural so, using 3D cell culture has an important role in the development
of effective drug testing models. Furthermore, we analyzed the ATP Binding Cassette Subfamily G Member
2 (ABCG2) gene and protein expression profile in this study. We aimed to establish a 3D breast cancer model that can
mimic the in vivo 3D breast cancer microenvironment.

Methods:
The 3D spheroid structures were multiplied (globally) using the three-dimensional hanging drop method.
The cultures of the parental cell line MDA-MB-231 served as the controls. After adding the drugs in different amounts,
we observed a clear and well-differentiated spheroid formation for 24 h. The viability and proliferation capacity of 2D
(sensitive and resistant) cell lines and 3D spheroid cell treatment were assessed by the XTT assay.

Results:
Cisplatin, Irinotecan, 5-Fu, and Gemcitabine-resistant MDA-MB-231 cells were observed to begin to disintegrate
in a three-dimensional clustered structure at 24 hours. Additionally, RT-PCR and protein assay showed overexpression
of ABCG2 when compared to the parental cell line. Moreover, MDA-MB-231 cells grown in 3D showed
decreased sensitivity to chemotherapeutics treatment.

Conclusion:
More resistance to chemotherapeutics and altered gene expression profile were shown in 3D cell cultures
when compared with the 2D cells. These results might play an important role to evaluate the efficacy of anticancer
drugs to explore the mechanisms of MDR in the 3D spheroid forms.
Imidazolidine Derivatives in Cancer Research: What is known?
Authors: da Silva Júnior Auvani Antunes , da Rocha Pitta Marina Galdino , de Oliveira Chagas Mardonny Bruno , de Melo Rêgo Moacyr Jesus Barreto , da Rosa Michelle Melgarejo , da Rocha Pitta Maira Galdino 
Published: July 2021

:
It is well known that cancer is the second leading cause of death worldwide. Due to this fact, new results for
the treatment of cancer are constantly being introduced and verified. Imidazolidine derivatives regulate cell cycle progression
and DNA stability. Structurally, a heterocyclic nucleus favors a direct DNA interaction and therefore, control
of the DNA replication process. This review aims not only to discuss the role of imidazolidines in cancer therapy but
also explore the functionality of such agents in the future aspects of cancer prognosis and treatment. Convincing data
from 1996 to 2021 has presented imidazolidine derivatives as a relevant therapeutic tool to modulate cancer progression
and malignancy. Here we highlight these aspects in a variety of cell lines, cancer types, involving in vitro and in
vivo techniques.
Molecular Mechanisms Underlying the Anti-Breast Cancer Stem Cell Activity of Pterocladia capillacea and Corallina officinalis Polysaccharides
Authors: Hafez Hebatallah G. , Mohareb Rafat M. , Salem Sohair M. , Matloub Azza A. , Eskander Emad F. , Ahmed Hanaa H. 
Published: July 2021

Objective:
This study aimed to appraise the activity of Pterocladia capillacea and Corallina officinalis
polysaccharides against Breast Cancer Stem Cells (BCSCs). P. capillacea and C. officinalis polysaccharides were
characterized to be sulfated polysaccharide-protein complexes.

Methods:
Cytotoxicity of the polysaccharides against MDA-MB-231 and MCF-7 cell lines along with their impact on
CD44+/CD24− and aldehyde dehydrogenase 1(ALDH1) positive BCSC population were determined. Their effect on
gene expression of CSC markers, Wnt/β-catenin and Notch signaling pathways was evaluated.

Results:
P. capillacea and C. officinalis polysaccharides inhibited the growth of breast cancer cells and reduced BCSC
subpopulation. P. capillacea polysaccharides significantly down-regulated OCT4, SOX2, ALDH1A3 and vimentin in
MDA-MB-231 as well as in MCF-7 cells except for vimentin that was up-regulated in MCF-7 cells. C. officinalis
polysaccharides exhibited similar effects except for OCT4 that was up-regulated in MDA-MB-231 cells. Significant
suppression of Cyclin D1 gene expression was noted in MDA-MB-231 and MCF-7 cells treated with P. capillacea or
C. officinalis polysaccharides. β-catenin and c-Myc genes were significantly down-regulated in MDA-MB-231 cells
treated with C. officinalis and P. capillacea polysaccharides, respectively, while being up-regulated in MCF-7 cells
treated with either of them. Additionally, P. capillacea and C. officinalis polysaccharides significantly down-regulated
Hes1 gene in MCF-7 cells despite increasing Notch1 gene expression level. However, significant down-regulation of
Notch1 gene was observed in MDA-MB-231 cells treated with P. capillacea polysaccharides.

Conclusion:
Collectively, this study provides evidence for the effectiveness of P. capillacea and C. officinalis polysaccharides
in targeting BCSCs through interfering with substantial signaling pathways contributing to their functionality.
Encouraging Hearing Loss Prevention in Music Listeners Using Personalized Technology: Questionnaire Study
Authors: Zhu Dylan Tianyu 
Published: July 2021

Background
Noise-induced hearing loss (NIHL) affects millions of people despite being almost completely preventable. For recreational music listening through personal listening equipment (such as earbuds), it seems that listeners do not yet have a way to accurately assess their risk of developing hearing loss and prevent it accordingly.

Objective
The aim of this study is to analyze the perceived utility of a hypothetical device that encourages NIHL prevention based on listeners’ exposure to noise and to determine the most effective methods of such encouragement. Here, we describe 3 different potential NIHL risk notification method types, as follows: auditory, external visual, and visual.

Methods
An open, web-based survey was created on Google Forms, and the link was posted to Amazon’s Mechanical Turk as well as music-related Reddit communities. The survey was designed to gauge each respondent’s self-assessed NIHL awareness, willingness to lower their audio if reminded, and NIHL risk notification type preference. The likelihood of a specific notification type to encourage NIHL prevention among its users was based on the average of each user’s responses to 2 survey questions. Data collection started on July 13, 2020, and ended on July 17, 2020.

Results
Of the 116 respondents, 92 (79.3%) reported having prior awareness about NIHL; however, 60 (51.7%) described doing nothing to prevent it despite 96 (82.8%) feeling a moderate, high, or extreme risk of developing NIHL. Of those who already prevented NIHL, 96% (53.5/56) described using estimates to guide their prevention instead of using data. A Kruskal-Wallis test corrected for ties showed that despite the visual NIHL risk notification type being selected by the highest number of participants (84/116, 72.4%), the auditory type had a significantly higher (H1=6.848; P=.03) average percentage likelihood of encouraging NIHL prevention (62%, SD 24%) among the 40 respondents who chose it, with a median likelihood of 56% (95% CI 50%-75%). The visual type’s average likelihood was 50% (SD 28.1%), with a median of 50% (95% CI 37.5%-56.3%). Regardless of the NIHL risk notification type, 69% (80/116) of respondents were not opposed to using NIHL risk notifications and lowering their audio volume accordingly.

Conclusions
The hypothetical device detailed here was thought to be useful because most respondents (82.8%, 96/116) felt an extreme to moderate risk of developing NIHL and such a device could provide accurate data to those who currently use estimates to prevent NIHL, and most respondents were willing to act on NIHL risk notifications. The most effective NIHL risk notification type seemed to be the auditory type, but many aspects of this study need further research to determine which implementation method should reach the public.
An Interactive Computer Game for Improving Selective Voluntary Motor Control in Children With Upper Motor Neuron Lesions: Development and Preliminary Feasibility Study
Authors: Fahr Annina , Kläy Andrina , Keller Jeffrey W , van Hedel Hubertus J A 
Published: July 2021

Background
Computer game–based interventions are emerging in pediatric neurorehabilitation, as they can provide two key elements for motor learning—motivating environments that enable long-term compliance, which is particularly relevant for children, and augmented feedback for improving movement performance.

Objective
The overall aim of this study is to develop an interactive computer play for children with upper motor neuron lesions to train selective voluntary motor control and give particular attention to motivation and feedback. We also aim to determine features that make games engaging, investigate which sensory feedback modality is noticed the fastest during play, develop an interactive game, and evaluate its feasibility.

Methods
We identified engaging game features by interviewing 19 children and adolescents undergoing rehabilitation. By using a test version of the game, we determined the response times of 10 patients who had to react to visual, auditory, or combined feedback signals. On the basis of the results of these two subprojects, we developed and designed a game environment. Feasibility was studied in terms of the practicability and acceptability of the intervention among 5 children with upper motor neuron lesions.

Results
The game features deemed the most important by pediatric patients were strategic gameplay (13/29, 45% of answers) and choice (6/29, 21%). While playing the game, an acoustic alarm signal (reaction time: median 2.8 seconds) was detected significantly faster (P=.01) than conditions with other feedback modalities (avatar velocity reduction: median 7.8 seconds; color desaturation: median 5.7 seconds). Most children enjoyed playing the game, despite some technical issues.

Conclusions
The careful identification of game features that increase motivation and feedback modalities that inform children quickly led to the development of an interactive computer play for training selective voluntary motor control in children and adolescents with upper motor neuron lesions.
The Use of Twitter by Medical Journals: Systematic Review of the Literature
Authors: Erskine Natalie , Hendricks Sharief 
Published: July 2021

Background
Medical journals use Twitter to engage and disseminate their research articles and implement a range of strategies to maximize reach and impact.

Objective
This study aims to systematically review the literature to synthesize and describe the different Twitter strategies used by medical journals and their effectiveness on journal impact and readership metrics.

Methods
A systematic search of the literature before February 2020 in four electronic databases (PubMed, Web of Science, Scopus, and ScienceDirect) was conducted. Articles were reviewed using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines.

Results
The search identified 44 original research studies that evaluated Twitter strategies implemented by medical journals and analyzed the relationship between Twitter metrics and alternative and citation-based metrics. The key findings suggest that promoting publications on Twitter improves citation-based and alternative metrics for academic medical journals. Moreover, implementing different Twitter strategies maximizes the amount of attention that publications and journals receive. The four key Twitter strategies implemented by many medical journals are tweeting the title and link of the article, infographics, podcasts, and hosting monthly internet-based journal clubs. Each strategy was successful in promoting the publications. However, different metrics were used to measure success.

Conclusions
Four key Twitter strategies are implemented by medical journals: tweeting the title and link of the article, infographics, podcasts, and hosting monthly internet-based journal clubs. In this review, each strategy was successful in promoting publications but used different metrics to measure success. Thus, it is difficult to conclude which strategy is most effective. In addition, the four strategies have different costs and effects on dissemination and readership. We recommend that journals and researchers incorporate a combination of Twitter strategies to maximize research impact and capture audiences with a variety of learning methods.
Safety and Acceptability of a Natural Language Artificial Intelligence Assistant to Deliver Clinical Follow-up to Cataract Surgery Patients: Proposal
Authors: de Pennington Nick , Mole Guy , Lim Ernest , Milne-Ives Madison , Normando Eduardo , Xue Kanmin , Meinert Edward 
Published: July 2021

Background
Due to an aging population, the demand for many services is exceeding the capacity of the clinical workforce. As a result, staff are facing a crisis of burnout from being pressured to deliver high-volume workloads, driving increasing costs for providers. Artificial intelligence (AI), in the form of conversational agents, presents a possible opportunity to enable efficiency in the delivery of care.

Objective
This study aims to evaluate the effectiveness, usability, and acceptability of Dora agent: Ufonia’s autonomous voice conversational agent, an AI-enabled autonomous telemedicine call for the detection of postoperative cataract surgery patients who require further assessment. The objectives of this study are to establish Dora’s efficacy in comparison with an expert clinician, determine baseline sensitivity and specificity for the detection of true complications, evaluate patient acceptability, collect evidence for cost-effectiveness, and capture data to support further development and evaluation.

Methods
Using an implementation science construct, the interdisciplinary study will be a mixed methods phase 1 pilot establishing interobserver reliability of the system, usability, and acceptability. This will be done using the following scales and frameworks: the system usability scale; assessment of Health Information Technology Interventions in Evidence-Based Medicine Evaluation Framework; the telehealth usability questionnaire; and the Non-Adoption, Abandonment, and Challenges to the Scale-up, Spread and Suitability framework.

Results
The evaluation is expected to show that conversational technology can be used to conduct an accurate assessment and that it is acceptable to different populations with different backgrounds. In addition, the results will demonstrate how successfully the system can be delivered in organizations with different clinical pathways and how it can be integrated with their existing platforms.

Conclusions
The project’s key contributions will be evidence of the effectiveness of AI voice conversational agents and their associated usability and acceptability.

International Registered Report Identifier (IRRID)
PRR1-10.2196/27227
Engaging Parents and Health Care Stakeholders to Inform Development of a Behavioral Intervention Technology to Promote Pediatric Behavioral Health: Mixed Methods Study
Authors: O'Dell Sean M , Fisher Heidi R , Schlieder Victoria , Klinger Tracey , Kininger Rachel L , Cosottile McKenna , Cummings Stacey , DeHart Kathy 
Published: July 2021

BackgroundDespite effective psychosocial interventions, gaps in access to care persist for youth and families in need. Behavioral intervention technologies (BITs) that apply psychosocial intervention strategies using technological features represent a modality for targeted prevention that is promising for the transformation of primary care behavioral health by empowering parents to take charge of the behavioral health care of their children. To realize the potential of BITs for parents, research is needed to understand the status quo of parental self-help and parent-provider collaboration to address behavioral health challenges and unmet parental needs that could be addressed by BITs.ObjectiveThe aim of this study is to conduct foundational research with parents and health care stakeholders (HCS) to discover current practices and unmet needs related to common behavioral health challenges to inform the design, build, and testing of BITs to address these care gaps within a predominantly rural health system.MethodsWe conducted a convergent mixed-parallel study within a large, predominantly rural health system in which the BITs will be developed and implemented. We analyzed data from parent surveys (N=385) on current practices and preferences related to behavioral health topics to be addressed in BITs along with focus group data of 48 HCS in 9 clinics regarding internal and external contextual factors contributing to unmet parental needs and current practices. By comparing and relating the findings, we formed interpretations that will inform subsequent BIT development activities.ResultsParents frequently endorsed several behavioral health topics, and several topics were relatively more or less frequently endorsed based on the child’s age. The HCS suggested that BITs may connect families with evidence-based guidance sooner and indicated that a web-based platform aligns with how parents already seek behavioral health guidance. Areas of divergence between parents and HCS were related to internalizing problems and cross-cutting issues such as parenting stress, which may be more difficult for health care HCS to detect or address because of the time constraints of routine medical visits.ConclusionsThese findings provide a rich understanding of the complexity involved in meeting parents’ needs for behavioral health guidance in a primary care setting using BITs. User testing studies for BIT prototypes are needed to successfully design, build, and test effective BITs to empower parents to take charge of promoting the behavioral health of their children.
The Transition to an Entirely Digital Immunization Registry in Ha Noi Province and Son La Province, Vietnam: Readiness Assessment Study
Authors: Duong Hong , Dao Sang , Dang Huyen , Nguyen Linh , Ngo Tuan , Nguyen Trung , Tran Lan Anh , Nguyen Doan , Rivera Maya , Nguyen Nga 
Published: July 2021

Background
Vietnam is one of the first low- to middle-income countries to develop and implement a national-scale electronic immunization registry. This system was finalized into the National Immunization Information System (NIIS) and scaled up to a national-level system in 2017. As a result, immunization coverage and the timeliness of vaccinations have drastically improved. The time spent on planning and reporting vaccinations has drastically reduced; as a result, vaccination planning and reporting has become more accurate and effective. However, to date, end users have been tasked with managing both the NIIS and paper-based systems in parallel until a formal assessment of the readiness to fully transition to the NIIS is conducted.

Objective
This study aims to evaluate the readiness to move to an entirely digital NIIS in 2 provinces of Vietnam—Ha Noi and Son La.

Methods
All health facilities were surveyed to assess their infrastructure, capacity, and need for human resources. NIIS end users were observed and interviewed to evaluate their NIIS knowledge and skill sets. Data from immunization cards and facility paper-based logbooks were compared with data from the NIIS, and vaccine stocks at selected facilities were tallied and compared with data from the NIIS.

Results
Of the 990 health facilities evaluated, most used the NIIS to enter and track immunizations (987/990, 99.7%) and vaccine stocks (889/990, 90.8%). Most had stable electricity (971/990, 98.1%), at least 1 computer (986/990, 99.6%), and ≥2 trained NIIS end users (825/990, 83.3%). End users reported that the NIIS supported them in managing and reporting immunization data and saving them time (725/767, 94.5%). Although many end users were able to perform basic skills, almost half struggled with performing more complex tasks. Immunization data were compiled from the NIIS and immunization cards (338/378, 89.4%) and paper-based logbooks (254/269, 94.4%). However, only 54.5% (206/378) of immunization IDs matched, 57% (13/23) of Bacillus Calmette-Guérin vaccination records were accurate, and 70% (21/30) of the facilities had consistent physical vaccine stock balances. The feedback received from NIIS end users suggests that more supportive supervision, frequent refresher training for strengthening their skill sets, and detailed standardized guides for improving data quality are needed.

Conclusions
The readiness to transition to a digital system is promising; however, additional resources are required to address the timeliness, completeness, and accuracy of the data.