bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skeletal Muscle Microvascular Dysfunction Manifests Early in Diabetic Cardiomyopathy
Authors: Loai Sadi , Zhou Yu-Qing , Vollett Kyle D. W. , Cheng Hai-Ling Margaret 
Published: July 2021

Aim: To perform a deep cardiac phenotyping of type II diabetes in a rat model, with the goal of gaining new insight into the temporality of microvascular dysfunction, cardiac dysfunction, and exercise intolerance at different stages of diabetes.Methods and Results: Diabetes was reproduced using a non-obese, diet-based, low-dose streptozotocin model in male rats (29 diabetic, 11 control). Time-course monitoring over 10 months was performed using echocardiography, treadmill exercise, photoacoustic perfusion imaging in myocardial and leg skeletal muscle, flow-mediated dilation, blood panel, and histology. Diabetic rats maintained a normal weight throughout. At early times (4 months), a non-significant reduction (30%) emerged in skeletal muscle perfusion and in exercise tolerance. At the same time, diabetic rats had a normal, slightly lower ejection fraction (63 vs. 71% control, p &lt; 0.01), grade 1 diastolic dysfunction (E/A = 1.1 vs. 1.5, isovolumetric relaxation time = 34 vs. 27 ms; p &lt; 0.01), mild systolic dysfunction (ejection time = 69 vs. 57 ms, isovolumetric contraction time = 21 vs. 17 ms; p &lt; 0.01), and slightly enlarged left ventricle (8.3 vs. 7.6 mm diastole; p &lt; 0.01). Diastolic dysfunction entered grade 3 at Month 8 (E/A = 1.7 vs. 1.3, p &lt; 0.05). Exercise tolerance remained low in diabetic rats, with running distance declining by 60%; in contrast, control rats ran 60% farther by Month 5 (p &lt; 0.05) and always remained above baseline. Leg muscle perfusion remained low in diabetic rats, becoming significantly lower than control by Month 10 (33% SO2 vs. 57% SO2, p &lt; 0.01). Myocardial perfusion remained normal throughout. Femoral arterial reactivity was normal, but baseline velocity was 25% lower than control (p &lt; 0.05). High blood pressure appeared late in diabetes (8 months). Histology confirmed absence of interstitial fibrosis, cardiomyocyte hypertrophy, or microvascular rarefaction in the diabetic heart. Rarefaction was also absent in leg skeletal muscle.Conclusion: Reduced skeletal muscle perfusion from microvascular dysfunction emerged early in diabetic rats, but myocardial perfusion remained normal throughout the study. At the same time, diabetic rats exhibited exercise intolerance and early cardiac dysfunction, in which changes related to heart failure with preserved ejection fraction (HFpEF) were seen. Importantly, skeletal muscle microvascular constrictionadvanced significantly before the late appearance of hypertension. HFpEF phenotypes such as cardiac hypertrophy, fibrosis, and rarefaction, which are typically associated with hypertension, were absent over the 10 month time-course of diabetes-related heart failure.
Inhibition of Lipoxygenases Showed No Benefit for the Musculoskeletal System in Estrogen Deficient Rats
Authors: Saul Dominik , Hohl Friederike Eva , Franz Max Konrad , Meyer Ilka , Taudien Stefan , Roch Paul Jonathan , Sehmisch Stephan , Komrakova Marina 
Published: July 2021

BackgroundIn previous studies, we reported the beneficial impact of two lipoxygenase-inhibitors, Baicalein and Zileuton, on osteoporotic bone in a postmenopausal rat model. Whereas subcutaneous Baicalein predominantly improved cortical bone, Zileuton enhanced vertebral and femoral trabecular bone. In this study, we aimed to reveal whether the oral administration of Baicalein caused similar effects on bone and whether a combined administration of Baicalein and Zileuton could act synergistically to ameliorate the formerly reported effects in the musculoskeletal system.MethodsWe treated ovariectomized (OVX) female Sprague-Dawley rats either with Baicalein (10mg/kg BW), Zileuton (10mg/kg BW) or a combination of both (each 10mg/kg BW) for 13 weeks and compared with untreated OVX and NON-OVX groups (n=12-16 rats per group). Lumbar vertebral bodies and femora were analyzed. Tibiae were osteotomized, plate-stabilized (at week 8 after OVX) and likewise analyzed by biomechanical, histological, micro-computed tomographical and ashing tests. The skeletal muscle structure was analyzed.ResultsOral administration of Baicalein did not confirm the reported favorable cortical effects in neither vertebra nor femur. Zileuton showed a beneficial effect on trabecular vertebra, while the femur was negatively affected. Callus formation was enhanced by all treatments; however, its density and biomechanical properties were unaltered. Lipoxygenase inhibition did not show a beneficial effect on skeletal muscle. The combination therapy did not ameliorate OVX-induced osteoporosis but induced even more bone loss.ConclusionsThe preventive anti-osteoporotic treatments with two lipoxygenase inhibitors applied either alone or in combination showed no benefit for the musculoskeletal system in estrogen deficient rats.
The N6-Methyladenosine-Modified Pseudogene HSPA7 Correlates With the Tumor Microenvironment and Predicts the Response to Immune Checkpoint Therapy in Glioblastoma
Authors: Zhao Rongrong , Li Boyan , Zhang Shouji , He Zheng , Pan Ziwen , Guo Qindong , Qiu Wei , Qi Yanhua , Zhao Shulin , Wang Shaobo , Chen Zihang , Zhang Ping , Guo Xing , Xue Hao , Li Gang 
Published: July 2021

BackgroundGlioblastoma (GBM), one of the most aggressive tumors of the brain, has no effective or sufficient therapies. Identifying robust biomarkers for the response to immune checkpoint blockade (ICB) therapy, a promising treatment option for GBM patients, is urgently needed.MethodsWe comprehensively evaluated lncRNA m6A modification patterns in m6A-sequencing (m6A-seq) data for GBM tissues and systematically investigated the immune and stromal regulators of these m6A-regulated lncRNAs. We used the single-sample gene-set enrichment analysis (ssGSEA) algorithm to investigate the difference in enriched tumor microenvironment (TME) infiltrating cells and the functional annotation of HSPA7 in individual GBM samples. Further, we validated that HSPA7 promoted the recruitment of macrophages into GBM TME in vitro, as well as in our GBM tissue section. We also explored its impact on the efficacy of ICB therapy using the patient-derived glioblastoma organoid (GBO) model.ResultsHere, we depicted the first transcriptome-wide m6A methylation profile of lncRNAs in GBM, revealing highly distinct lncRNA m6A modification patterns compared to those in normal brain tissues. We identified the m6A-modified pseudogene HSPA7 as a novel prognostic risk factor in GBM patients, with crucial roles in immunophenotype determination, stromal activation, and carcinogenic pathway activation. We confirmed that HSPA7 promoted macrophage infiltration and SPP1 expression via upregulating the YAP1 and LOX expression of glioblastoma stem cells (GSCs) in vitro and in our clinical GBM tumor samples. We also confirmed that knockdown of HSPA7 might increase the efficiency of anti-PD1 therapy utilizing the GBO model, highlighting its potential as a novel target for immunotherapy.ConclusionsOur results indicated that HSPA7 could be a novel immunotherapy target for GBM patients.
Dysfunctional Brain Dynamics of Parkinson's Disease and the Effect of Acute Deep Brain Stimulation
Authors: Li Zhibao , Ren Guoping , Liu Chong , Wang Qiao , Liang Kun , Han Chunlei , Qiao Hui , Zhang Jianguo , Wang Qun , Meng Fangang 
Published: July 2021

Background: Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, and deep brain stimulation (DBS) can effectively alleviate PD symptoms. Although previous studies have detected network features of PD and DBS, few studies have considered their dynamic characteristics.Objective: We tested two hypotheses. (1) Reduced brain dynamics, as evidenced by slowed microstate dynamic change, is a characteristic of PD and is related to the movement disorders of patients with PD. (2) Therapeutic acute DBS can partially reverse slow brain dynamics in PD to healthy levels.Methods: We used electroencephalography (EEG) microstate analysis based on high density (256-channel) EEG to detect the effects of PD and DBS on brain dynamic changes on a sub-second timescale. We compared 21 healthy controls (HCs) with 20 patients with PD who were in either DBS-OFF or DBS-ON states. Assessment of movement disorder using the Unified Parkinson's Disease Rating Scale III was correlated with microstate parameters.Results: Compared with HCs, patients with PD displayed a longer mean microstate duration with reduced occurrence per second, which were significantly associated with movement disorders. In patients with PD, some parameters of microstate analysis were restored toward healthy levels after DBS.Conclusions: Resting-state EEG microstate analysis is an important tool for investigating brain dynamic changes in PD and DBS. PD can slow down brain dynamic change, and therapeutic acute DBS can partially reverse this change toward a healthy level.
Case Report: Discovery a Novel SARS-CoV-2 Variant in a Six-Months Long-Term Swab Positive Female Suffering From Non-Hodgkin Lymphoma
Authors: Capoluongo Ettore , Nardelli Carmela , Esposito Maria Valeria , Buonomo Antonio Riccardo , Gelzo Monica , Pinchera Biagio , Zappulo Emanuela , Viceconte Giulio , Portella Giuseppe , Setaro Mario , Gentile Ivan , Castaldo Giuseppe 
Published: July 2021

BackgroundWe report the case of a woman with non-Hodgkin lymphoma who remained positive on the molecular assay for SARS-CoV-2 for six months: she has never experienced a severe form of COVID-19 although in absence of seroconversion.MethodsThe whole SARS-CoV-2 genome analysis was performed by the CleanPlex SARS-CoV-2 Research and Surveillance NGS Panel (PARAGON GENOMICS, Hayward, USA).ResultsWe found twenty-two mutations in SARS-CoV-2 genome and a novel deleterious ORF3a frameshift c.766_769del corresponding to a unique and novel lineage. The region affected by this frameshift variant is reported as being important in determining SARS-CoV-2 immunogenicity. Patient’s immunophenotype showed the absence of B lymphocytes and significantly reduced T-cell count. Only after the treatment with hyperimmune plasma she finally became negative on the swab.ConclusionsOur findings could be helpful in the management of patients with immunodeficiency, particularly when novel variants, potentially altering the virus immune response, are present.
The Impact of Angiotensin-Converting Enzyme Inhibitors or Angiotensin II Receptor Blockers on Clinical Outcomes of Acute Kidney Disease Patients: A Systematic Review and Meta-Analysis
Authors: Chen Jui-Yi , Tsai I-Jung , Pan Heng-Chih , Liao Hung-Wei , Neyra Javier A. , Wu Vin-Cent , Chueh Jeff S. 
Published: July 2021

Background: Acute kidney injury (AKI) may increase the risk of chronic kidney disease (CKD), development of end-stage renal disease (ESRD), and mortality. However, the impact of exposure to angiotensin-converting enzyme inhibitor or angiotensin II receptor blocker (ACEi/ARB) in patients experiencing AKI/acute kidney disease (AKD) is still unclear.Methods: In this systematic review, we searched all relevant studies from PubMed, Embase, Cochrane, Medline, Collaboration Central Register of Controlled Clinical Trials, Cochrane Systematic Reviews, and ClinicalTrials.gov until July 21, 2020. We evaluated whether the exposure to ACEi/ARB after AKI onset alters recovery paths of AKD and impacts risks of all-cause mortality, recurrent AKI, or incident CKD. We rated the certainty of evidence according to Cochrane methods and the GRADE approach.Results: A total of seven articles, involving 70,801 patients, were included in this meta-analysis. The overall patient mortality rate in this meta-analysis was 28.4%. Among AKI patients, all-cause mortality was lower in ACEi/ARB users than in ACEi/ARB nonusers (log odds ratio (OR) −0.37, 95% confidence interval (CI): −0.42–−0.32, p &lt; 0.01). The risk of recurrent adverse kidney events after AKI was lower in ACEi/ARB users than in nonusers (logOR −0.25, 95% CI: −0.33–−0.18, p &lt; 0.01). The risk of hyperkalemia was higher in ACEi/ARB users than in nonusers (logOR 0.43, 95% CI: 0.27–0.59, p &lt; 0.01). Patients with continued use of ACEi/ARB after AKI also had lower mortality risk than those prior ACEi/ARB users but who did not resume ACEi/ARB during AKD (logOR −0.36, 95% CI: −0.4–−0.31, p &lt; 0.01).Conclusions: Exposure to ACEi/ARB after AKI is associated with lower risks of all-cause mortality, recurrent AKI, and progression to incident CKD. Patients with AKI may have a survival benefit by continued use of ACEi/ARB; however, a higher incidence of hyperkalemia associated with ACEi/ARB usage among these patients deserves close clinical monitoring.
Gender-Based Analysis of the Psychological Impact of the COVID-19 Pandemic on Healthcare Workers in Spain
Authors: López-Atanes Mayte , Pijoán-Zubizarreta José Ignacio , González-Briceño Juan Pablo , Leonés-Gil Elena María , Recio-Barbero María , González-Pinto Ana , Segarra Rafael , Sáenz-Herrero Margarita 
Published: July 2021

Purpose: This study aims to analyze from a gender perspective the psychological distress experienced by the medical workforce during the peak of the pandemic in Spain.Methods: This is a single-center, observational analytic study. The study population comprised all associated health workers of the Cruces University Hospital, invited by email to participate in the survey. It consisted of a form covering demographic data, the general health questionnaire-28 (GHQ-28), and the perceived stress scale (PSS-14). We used multivariant regression analysis to check the effect of gender on the scores. We used gender analysis in both design and interpretation of data following SAGER guidelines.Results: Females made 74.6% of our sample, but their proportion was higher in lower-paid positions such as nursery (89.9%) than in higher-paid ones. The percentage of women categorized as cases with the GHQ-28 was 78.4%, a proportion significantly higher than in the male population (61.3%, p &lt; 0.001). The multivariant regression analysis showed that being women, working as orderly hospital porters, and having a past psychiatric history were risk factors for higher scores in both the GHQ-28 and PSS-14.Conclusion: Women and those with lower-paid positions were at risk of higher psychological distress and worse quality of life within the medical workforce during the first wave of the pandemic. Gender analysis must be incorporated to analyze this fact better.
Ischemic Stroke Risk Assessment by Multiscale Entropy Analysis of Heart Rate Variability in Patients with Persistent Atrial Fibrillation
Authors: Chairina Ghina , Yoshino Kohzoh , Kiyono Ken , Watanabe Eiichi 
Published: July 2021

It has been recognized that heart rate variability (HRV), defined as the fluctuation of ventricular response intervals in atrial fibrillation (AFib) patients, is not completely random, and its nonlinear characteristics, such as multiscale entropy (MSE), contain clinically significant information. We investigated the relationship between ischemic stroke risk and HRV with a large number of stroke-naïve AFib patients (628 patients), focusing on those who had never developed an ischemic/hemorrhagic stroke before the heart rate measurement. The CHA2DS2−VASc score was calculated from the baseline clinical characteristics, while the HRV analysis was made from the recording of morning, afternoon, and evening. Subsequently, we performed Kaplan–Meier method and cumulative incidence function with mortality as a competing risk to estimate the survival time function. We found that patients with sample entropy (SE(s)) ≥ 0.68 at 210 s had a significantly higher risk of an ischemic stroke occurrence in the morning recording. Meanwhile, the afternoon recording showed that those with SE(s) ≥ 0.76 at 240 s and SE(s) ≥ 0.78 at 270 s had a significantly lower risk of ischemic stroke occurrence. Therefore, SE(s) at 210 s (morning) and 240 s ≤ s ≤ 270 s (afternoon) demonstrated a statistically significant predictive value for ischemic stroke in stroke-naïve AFib patients.
Pelo prisma da estética
Authors: Arantes Otília Beatriz Fiori 
Published: July 2021

Entrevista concedida à revista Rapsódia, do Departamento de Filosofia da FFLCH-USP, em 2002. Na entrevista, Otília refaz sua trajetória acadêmica e intelectual, ao mesmo tempo em que vai expondo seu pensamento a respeito de vários temas a que se dedicara até então, da crítica de Mário Pedrosa à arte pós-moderna, do esgotamento da Arquitetura Moderna à centralidade da cultura nas novas estratégias urbanas, os novos museus, a indústria cultural e a cultura do espetáculo, o lugar da crítica e, a questão central, presente ao longo da entrevista: a morte ou a sobrevida da arte, por consequência, da Estética.
Publicado originalmente em 2002 na Revista Rapsódia: almanaque de filosofia e arte, n.2. São Paulo: Departamento de Filosofia FFLCH-USP, p. 221-264.
Palavras-chave: Estética, FFLCH USP, CEAC, Artes plásticas, Arquitetura Moderna, Gilda de Mello e Souza, Mário Pedrosa, Charles Baudelaire, Habermas, Roberto Schwarz, Experiência estética, Morte da Arte, Hegel, Espetáculo, Cidades, Guy Débord, Adorno, Museus, Curadoria, Moda
Manfredo Tafuri
Authors: Fiori Arantes Otília Beatriz 
Published: July 2021

Foram escolhidas, dentre as várias falas, aulas e entrevistas de Otília Arantes, dedicadas a Manfredo Tafuri, duas palestras, pronunciadas em dois eventos acadêmicos da USP: a primeira, de 1994, uma homenagem de Otília ao arquiteto, historiador e crítico, logo após sua morte, num seminário sobre História da Cidade no campus de São Carlos, e a segunda, vinte anos depois, proferida num seminário internacional, “Manfredo Tafuri, seus leitores e suas leituras”, na FAU (fevereiro de 2015). Ambas tomando como foco principal seu livro Progetto e Utopia: Architettura e Sviluppo Capitalistico. A primeira, “A Arquitetura da Cidade: um ponto de vista”, após situar o Autor no contexto dos estudos sobre Arquitetura e Urbanismo na Itália dos anos 1960/70, procede a uma minuciosa análise de texto do livro citado, especialmente no seu diagnóstico sobre o esgotamento do Movimento Moderno, cada vez mais profundamente ligado, segundo Tafuri, à cidade como estrutura produtiva, portanto, reduzido a “mecanismo operativo” e, sua “ideologia do plano”, a uma “utopia regressiva”. Seguem-se algumas considerações sobre as manifestações posteriores, ditas pós-modernas, cuja crítica leva Tafuri a propor, como alternativa ao historicismo dominante, um “niilismo compiuto”. A interpretação deste último conceito, tomado naquele momento como expressão de um certo “pessimismo paralisante”, é retomada, passadas duas décadas, ao final da segunda exposição, mas já numa leitura que a própria Otília considera mais justa, interpretando-a como consequência natural de uma “filosofia da negação”. Este, aliás, o tema da conferência: “A dialética negativa de Manfredo Tafuri”, em que a Autora procura reconstituir, em diálogo com Asor Rosa, as raízes marxistas de Tafuri, seu ponto de vista dialético, apenas sugerido na primeira fala, ou melhor, sua inserção na tradição marxista italiana, temperada, ao menos a partir de um certo momento, pela influência de um pensamento que começava a se difundir na Itália à época, ao menos no meio acadêmico italiano (anos 1960/70): a Teoria Crítica da Escola de Frankfurt – sem desprezar outras referências que compõem o que ela chama de “marxismo heterodoxo” ou de “saudável ecletismo” do pensamento “nada linear” de Tafuri.
O link para o vídeo desta última conferência é disponibilizado em botão abaixo dos ebooks para download.

Palavras-chave: Vanguardas, Metrópole, Movimento Moderno, Ideologia do plano, Utopia, Antolini, Laugier, Corbusier, Bauhaus, Arquitetura italiana, Niilismo, Semiologia, Estruturalismo, Nietzsche, Foucault, Método genealógico, Ginzburg, Asor Rosa, Marxismo, Dialética, Walter Benjamin, Adorno, Brecht
WIRELESS LOCAL AREA NETWORKS IN A PERSONALIZED EMERGENCY RESCUE MANAGEMENT SYSTEM
Authors: САРЬЯН В.К. , ПАРАМОНОВ А.И. , ВИКУЛОВ А.С. , ЯКУБОВСКИЙ Р.М. 
Published: July 2021

Анализируются возможности использования локальных беспроводных сетей широкополосного доступа в системах управления спасением людей в чрезвычайных ситуациях. Рассматриваются методы локализации пользователей, оказавшихся в опасных условиях, а также способы повышения доступности информации о них для передачи в центр управления спасением и уровня информированности самих пользователей о планах центра по их спасению. Предлагается оригинальное решение, позволяющее выполнять локализацию поэлементно, т. е. в помещениях и на отдельных участках области обслуживания системы. Показано, что решение может быть успешно использовано для организации сетевой инфраструктуры объектовой части глобальной экспертной системы, обеспечивающей доступ массового абонента в любое время и в любом месте к услуге индивидуализированного управления спасением абонентов при возникновении чрезвычайной ситуации природного или техногенного происхождения. Представлены методы повышения устойчивости сети, в частности метод построения одноранговой ad-hoc-сети с использованием коммуникаций устройство-устройство.
The article analyzes the possibilities of using local wireless broadband access networks in emergency management systems for rescue people. Methods of localization of users in dangerous conditions, ways of increasing the availability of information about them for the rescue control center, as well as the level of awareness of the users themselves about the plans of the center for their rescue are considered. It is proposed the original solution, that allows localization to be performed element by element, i.e. in rooms and certain areas of the system service area. It is shown that the solution can be successfully used to organize the network infrastructure of the facility part of the global expert system, which provides mass subscriber access at any time and in any place to the service of individualized management of the rescue of subscribers in case of natural or man-made disasters. Methods for enhancing network stability are presented, in particular, a method for constructing a peer-to-peer ad-hoc network using device-to-device communications.
ORGANIZATION OF INFORMATION SYSTEMS OPERATION SUPPORT BASED ON THE ANALYSIS OF USER REQUESTS
Authors: БАКАНОВА Н.Б. , АТАНАСОВА Т.В. 
Published: July 2021

Рассматривается метод автоматизированного анализа обращений пользователей, поступающих в Центр обслуживания крупномасштабной распределенной информационной сети. Метод базируется на выявлении проблематики поступающих обращений и предложений, связанных с функционированием информационных комплексов организации. Проведено исследование по функционированию сети на основе анализа обращений пользователей. Показано, что оперативная обработка обращений и принятие решений по их исполнению позволит обеспечить эффективное обслуживание информационной сети организации.
A method of automated analysis of user requests arriving at the service center of a large-scale distributed information network isconsidered. The method is based on identifying the problems of incoming requests and proposals related to the functioning of the organization's information systems. A study on the functioning of the network based on the analysis of user requestswas carried out. It isshownthat the promptprocessing of requests and decision-making on their execution will ensure effective maintenance of the organization's information network.