bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forensic analysis of beverage stains using hyperspectral imaging
Authors: Melit Devassy Binu , George Sony 
Published: March 2021

AbstractDocumentation and analysis of crime scene evidences are of great importance in any forensic investigation. In this paper, we present the potential of hyperspectral imaging (HSI) to detect and analyze the beverage stains on a paper towel. To detect the presence and predict the age of the commonly used drinks in a crime scene, we leveraged the additional information present in the HSI data. We used 12 different beverages and four types of paper hand towel to create the sample stains in the current study. A support vector machine (SVM) is used to achieve the classification, and a convolutional auto-encoder is used to achieve HSI data dimensionality reduction, which helps in easy perception, process, and visualization of the data. The SVM classification model was re-established for a lighter and quicker classification model on the basis of the reduced dimension. We employed volume-gradient-based band selection for the identification of relevant spectral bands in the HSI data. Spectral data recorded at different time intervals up to 72 h is analyzed to trace the spectral changes. The results show the efficacy of the HSI techniques for rapid, non-contact, and non-invasive analysis of beverage stains.
Moderate vs. mild cases of overseas-imported COVID-19 in Beijing: a retrospective cohort study
Authors: Zhai Wenliang , Luo Zujin , Zheng Yue , Dong Dawei , Wu Endong , Wang Zhengfang , Zhai Junpeng , Han Yujuan , Liu Huan , Wang Yanran , Feng Yaohui , Wang Jing , Ma Yingmin 
Published: March 2021

AbstractThis study compared the differences in the clinical manifestations, treatment courses and clinical turnover between mild and moderate coronavirus disease 2019 (COVID-19). Clinical data of the patients with imported COVID-19 admitted to Beijing Xiaotangshan Designated Hospital between March 15 and April 30, 2020, were retrospectively analysed. A total of 53 COVID-19 patients were included, with 21 mild and 32 moderate cases. Compared with the mild group, the moderate group showed significant differences in breathing frequency, lymphocyte count, neutrophil percentage, neutrophil/lymphocyte ratio, procalcitonin, C-reactive protein, and dynamic erythrocyte sedimentation rate. In the moderate group, 87.5% exhibited ground-glass opacities, 14% exhibited consolidative opacities, 53.1% exhibited local lesions and 68.8% exhibited unilateral lesions. The proportion of patients who received antiviral or antibiotic treatment in the moderate group was higher than that in the mild group, and the number of cases that progressed to severe disease in the moderate group was also significantly higher (18.7% vs. 0%, p = 0.035). Compared with patients with mild COVID-19, those with moderate COVID-19 exhibited more noticeable inflammatory reactions, more severe pulmonary imaging manifestations and earlier expression of protective antibodies. The overall turnover of the moderate cases was poorer than that of the mild cases.
Advanced bioinformatic analysis and pathway prediction of NSCLC cells upon cisplatin resistance
Authors: Hossian A K M Nawshad , Zahra Fatema Tuz , Poudel Sagun , Abshire Camille F. , Polk Paula , Garai Jone , Zabaleta Jovanny , Mikelis Constantinos M. , Mattheolabakis George 
Published: March 2021

AbstractThis study aims to identify pathway involvement in the development of cisplatin (cis-diamminedichloroplatinum (II); CDDP) resistance in A549 lung cancer (LC) cells by utilizing advanced bioinformatics software. We developed CDDP-resistant A549 (A549/DDP) cells through prolonged incubation with the drug and performed RNA-seq on RNA extracts to determine differential mRNA and miRNA expression between A549/DDP and A549 cells. We analyzed the gene dysregulation with Ingenuity Pathway Analysis (IPA; QIAGEN) software. In contrast to prior research, which relied on the clustering of dysregulated genes to pathways as an indication of pathway activity, we utilized the IPA software for the dynamic evaluation of pathway activity depending on the gene dysregulation levels. We predicted 15 pathways significantly contributing to the chemoresistance, with several of them to have not been previously reported or analyzed in detail. Among them, the PKR signaling, cholesterol biosynthesis, and TEC signaling pathways are included, as well as genes, such as PIK3R3, miR-34c-5p, and MDM2, among others. We also provide a preliminary analysis of SNPs and indels, present exclusively in A549/DDP cells. This study's results provide novel potential mechanisms and molecular targets that can be explored in future studies and assist in improving the understanding of the chemoresistance phenotype.
Investigation of thermal changes in the thyroid gland region of individuals with hypothyroidism and fibromyalgia by analyzing the temperature of brown adipose tissue
Authors: Costa Ana Paula Christakis , Maia Joaquim Miguel , Brioschi Marcos Leal , de Melo Mafra Machado José Eduardo 
Published: March 2021

AbstractThis exploratory retrospective study aims to investigate the thermal changes in the thyroid gland region of patients with hypothyroidism and fibromyalgia by analyzing the temperature of the brown adipose tissue (BAT). A total of 166 individuals from 1000 thermographic electronic medical records were classified into four groups: Group HP + FM-50 individuals with hypothyroidism and fibromyalgia; Group FM-56 individuals with fibromyalgia only; Group HP-30 individuals with hypothyroidism only, and Group Control-30 healthy individuals. The thermal images from the electronic medical records were acquired by a FLIR T650SC infrared camera (used for thermometry) and the temperature data for each group were statistically analyzed. Group HP + FM showedr = 0, meaning that the average temperatures of the thyroid and BAT are independent of each other. Groups FM, HP and Control showedr = 1, meaning that the average temperatures of the thyroid and BAT were directly related. Our findings showed that the average temperatures of the thyroid and BAT regions are similar. Also, there was no correlation between thyroid gland temperature and the presence of hypothyroidism or fibromyalgia using thermometry.
The potential value of serum chemerin in patients with breast cancer
Authors: Song Yanfang , Zhu Xianjin , Lin Zhen , Luo Li , Wen Dan 
Published: March 2021

AbstractBreast cancer (BC) is one of the most dangerous malignant diseases in females. However, the reliable serum biomarkers of BC still need to be explored. Chemerin levels have been found to be associated with different types of cancer. This study aimed to evaluate the role of serum chemerin as a biomarker of BC diagnosis, as well as the correlation between serum chemerin levels and clinicopathological features. The serum from 248 BC patients, 30 breast benign tumor patients, and 103 healthy controls were collected and serum chemerin levels were determined with enzyme-linked immunosorbent assay. We found that serum levels of chemerin in BC patients were higher than those in healthy control individuals (p < 0.05). The area under the ROC curve (AUC) for chemerin, CA15-3 and CEA was 0.703, 0.662 and 0.581, respectively, in distinguishing between breast cancer patients from healthy individuals, and the chemerin cutoff value was 100.327 ng/ml with a sensitivity of 56.60% and a specificity of 98.10%. The AUC for chemerin + CA15-3 was 0.822, which was higher than that for chemerin + CEA and CEA + CA15-3. Moreover, serum levels of chemerin were significantly associated with histologic grade, Ki67 expression, and menopausal status. However, no significant association was found between serum levels of chemerin and age, tumor size, metastase, ER status, PR status, and HER-2 status. Overall, our study suggested that the combination of chemerin with CA15-3 achieves relatively better diagnostic performance in the breast cancer. Elevated serum chemerin is associated with Ki67 expression levels and histologic grade.
Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
Authors: Belay Birhanu , Koivisto Janne T. , Parraga Jenny , Koskela Olli , Montonen Toni , Kellomäki Minna , Figueiras Edite , Hyttinen Jari 
Published: March 2021

AbstractAssessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the living cells. This is difficult using established optical microscopy techniques such as wide-field or confocal microscopy. To address the problem, we have developed a new protocol using Optical Projection Tomography (OPT) to extract quantitative and qualitative measurements from hydrogel cell cultures. Using our tools, we demonstrated the method by analyzing cell response in three different hydrogel formulations in 3D with 1.5 mm diameter samples of: gellan gum (GG), gelatin functionalized gellan gum (gelatin-GG), and Geltrex. We investigated cell morphology, density, distribution, and viability in 3D living cells. Our results showed the usability of the method to quantify the cellular responses to biomaterial environment. We observed that an elongated morphology of cells, thus good material response, in gelatin-GG and Geltrex hydrogels compared with basic GG. Our results show that OPT has a sensitivity to assess in real 3D cultures the differences of cellular responses to the properties of biomaterials supporting the cells.
Investigating the emergence of sex differences in jealousy responses in a large community sample from an evolutionary perspective
Authors: Larsen Per Helge H. , Bendixen Mons , Grøntvedt Trond Viggo , Kessler Andrea M. , Kennair Leif Edward Ottesen 
Published: March 2021

AbstractSex differences in jealousy responses to sexual and emotional infidelity are robust in samples of heterosexual adults, especially in more gender egalitarian nations. However, investigations of when and how these differences develop have been scant. We applied two forced choice infidelity scenarios in a large community sample of high school students (age 16–19, N = 1266). In line with previous findings on adults using the forced choice paradigm, adolescent males found the sexual aspect of imagined infidelity more distressing than adolescent females did. Nevertheless, there was no effect of age on the jealousy responses, and age did not moderate the sex difference. There were neither any effects of three covariates (having had first sexual intercourse, being in a committed romantic relationship, and sociosexuality), neither as markers of pubertal maturation nor as psychosocial environmental stimuli. Future research needs to investigate even younger samples in order to specify at what age the sex difference in jealousy responses emerges.
Combination of arbuscular mycorrhizal fungi and phosphate solubilizing bacteria on growth and production of Helianthus tuberosus under field condition
Authors: Nacoon Sabaiporn , Jogloy Sanun , Riddech Nuntavun , Mongkolthanaruk Wiyada , Ekprasert Jindarat , Cooper Julia , Boonlue Sophon 
Published: March 2021

AbstractIn this work, the effects of co-inoculation between an arbuscular mycorrhizal fungus (AMF) and a phosphate solubilizing bacteria (PSB) to promote the growth and production of sunchoke under field condition were investigated during 2016 and 2017. Four treatments were set up as follows: plants without inoculation, with AMF inoculation, with PSB inoculation and with co-inoculation of PSB and AMF. The results showed the presence of PSB and AMF colonization at the harvest stage in both years. This suggested the survival of PSB and successful AMF colonization throughout the experiments. According to correlation analysis, PSB positively affected AMF spore density and colonization rate. Also, both AMF and PSB positively correlated with growth and production of sunchoke. Co-inoculation could enhance various plant parameters. However, better results in 2016 were found in co-inoculation treatment, while AMF inoculation performed the best in 2017. All of these results suggested that our AMF and PSB could effectively promote growth and production of sunchoke under field conditions. Such effects were varied due to different environmental conditions each year. Note that this is the first study showing successful co-inoculation of AMF and PSB for promoting growth and yield of sunchoke in the real cultivation fields.
High-performance gallium nitride dielectric metalenses for imaging in the visible
Authors: Chen Meng-Hsin , Chou Wei-Ning , Su Vin-Cent , Kuan Chieh-Hsiung , Lin Hoang Yan 
Published: March 2021

AbstractMetalens is one of the most promising applications for the development of metasurfaces. A wide variety of materials have been applied to metalenses working at certain spectral bands in order to meet the requirements of high efficiency and low-cost fabrication. Among these materials, wide-bandgap gallium nitride (GaN) is one of the most promising materials considering its advantages especially in semiconductor manufacturing. In this work, GaN has been utilized to fabricate the high-performance metalenses operating at visible wavelengths of 405, 532, and 633 nm with efficiencies up to 79%, 84%, and 89%, respectively. The homemade 1951 United State Air Force (UASF) resolution test chart has also been fabricated in order to provide resolvable lines with widths as small as 870 nm. As shown in the experimental results for imaging, the metalens designed at 405 nm can provide extremely high resolution to clearly resolve the smallest lines with the nano-sized widths in the homemade resolution test chart. These extraordinary experimental results come from our successful development in design and fabrication for the metalenses composed of high-aspect-ratio GaN nanoposts with nearly vertical sidewalls.
Ocular manifestations and biomarkers of Gulf War Illness in US veterans
Authors: Baksh Brandon S. , Zayan Kristen L. , Goldhardt Raquel , Felix Elizabeth R. , Klimas Nancy , Galor Anat 
Published: March 2021

AbstractGulf War Illness (GWI) is a multisystem disease with variable presentations, making diagnosis difficult. Non-invasive biomarkers would aid in disease diagnosis. We hypothesized that the eye could serve as a biomarker for GWI. We performed a retrospective case–control study using a sample of 1246 patients seen during a 5-month period in an optometry clinic. We identified veterans who were active duty during the Gulf War Era and either had a questionnaire-based diagnosis of GWI (cases) or did not (controls). Medical records were reviewed for eye and medical co-morbidities, medication use, and retinal macular and nerve fiber layer (NFL) thicknesses based on optical coherence tomography (OCT) images. Compared to controls (n = 85), individuals with GWI (n = 60) had a higher frequency of dry eye symptoms (50% vs 32.9%, p = 0.039). Multivariable analysis revealed average retinal NFL thickness (odds ratio; OR = 0.95), cup-to-disc ratio (OR = 0.005), age (OR = 0.82), and PTSD (OR = 20.5) were predictors of a GWI diagnosis. We conclude that GWI is associated with dry eye symptoms and RNFL thinning may serve as a biomarker for disease.
Comorbidities associated with genetic abnormalities in children with intellectual disability
Authors: Chen Jia-Shing , Yu Wen-Hao , Tsai Meng-Che , Hung Pi-Lien , Tu Yi-Fang 
Published: March 2021

AbstractIntellectual disability (ID) has emerged as the commonest manifestation of underlying genomic abnormalities. Given that molecular genetic tests for diagnosis of ID usually require high costs and yield relatively low diagnostic rates, identification of additional phenotypes or comorbidities may increase the genetic diagnostic yield and are valuable clues for pediatricians in general practice. Here, we enrolled consecutively 61 children with unexplained moderate or severe ID and performed chromosomal microarray (CMA) and sequential whole-exome sequencing (WES) analysis on them. We identified 13 copy number variants in 12 probands and 24 variants in 25 probands, and the total diagnostic rate was 60.7%. The genetic abnormalities were commonly found in ID patients with movement disorder (100%) or with autistic spectrum disorder (ASD) (93.3%). Univariate analysis showed that ASD was the significant risk factor of genetic abnormality (P = 0.003; OR 14, 95% CI 1.7–115.4). At least 14 ID-ASD associated genes were identified, and the majority of ID-ASD associated genes (85.7%) were found to be expressed in the cerebellum based on database analysis. In conclusion, genetic testing on ID children, particularly in those with ASD is highly recommended. ID and ASD may share common cerebellar pathophysiology.
Commentary on: Calpain-2 participates in the process of calpain-1 inactivation
Authors: Kumari Raniki , Maiti Tushar Kanti 
Published: March 2021

Abstract
Calpain belongs to the calcium-dependent non-lysosomal cysteine protease. Calpain-1 (C1) and calpain-2 (C2) expression are ubiquitous in mammals and an important mediator of the action of calcium. Specific substrate cleavage by C1 and C2 is critical for several calcium-dependent cellular pathways including neuronal function, muscle contraction, signal transduction, cell differentiation, proliferation, and apoptosis. Research suggests that C1 and C2 perform similar functions due to their structurally highly similar isoforms. Increasing evidence suggests that C1 and C2 carry out their specific function in vivo. A recent paper published by Shinkai-Ouchi et al. (Bioscience Reports (2020) 40, DOI: 10.1042/BSR20200552) elucidated the mechanism to differentiate the function of each calpain with respect to the efficiency and longevity for proteolysis after activation. Further, the study represented that C1 and C2 do not synergistically perform their work in vitro. On the other hand, the activity of C1 is reduced in presence of C2. This insight establishes the platform for future studies to examine how C2 regulates the C1 for substrate proteolysis.
Making the Right Connections: Maximizing Lexical Generalization in Lexical Impairments in Primary Progressive Aphasia and Alzheimer's Disease
Authors: Beales Ashleigh , Whitworth Anne , Cartwright Jade , Panegyres Peter K. , Kane Robert T. 
Published: March 2021

Purpose
Positive intervention effects following lexical retrieval interventions are increasingly reported with people with progressive language impairments; however, generalization of therapy gains are less frequently evident and less well understood. This study sought to explore the impact of specific therapy ingredients on generalization outcomes.

Method
Twelve participants with progressive lexical retrieval deficits (four each with semantic variant primary progressive aphasia, logopenic variant primary progressive aphasia, and Alzheimer's disease, amnestic presentation) and their family members participated in a 6-week intervention that aimed to increase access to different word classes (nouns, verbs, and adjectives) through a strategic self-cueing approach. Generalization was actively facilitated through strategy practice in connected speech. Repeated baselines of picture naming and connected speech were conducted prior to intervention and repeated immediately post and at 6 weeks following intervention.

Results
All three diagnostic groups showed significant improvements in naming performance post-intervention for all word classes and for both treated and untreated items, demonstrating consistent treatment effectiveness and generalization at the word level. No changes in the informativeness or efficiency of connected speech were found.

Conclusions
Despite heterogeneity across participants, widespread evidence of both treatment effects and generalization to untreated items was found for all diagnostic groups and word classes. The consistent within-level generalization across all groups is explored here in relation to optimization of strategy use through incorporation of cognitive scaffolds, strategic practice at the connected speech level, and the inclusion of family members. The absence of across-level generalization to connected speech is also explored.

Supplemental Material

https://doi.org/10.23641/asha.14219771
Co‐optimisation of wind farm micro‐siting and cabling layouts
Authors: Al Shereiqi A. , Mohandes B. , Al‐Hinai A. , Bakhtvar M. , Al‐Abri R. , El Moursi M. S. , Albadi M. 
Published: March 2021

AbstractWind farm layout optimisation (WFLO) is carried out in this study considering the wake effect, and cabling connections and losses. The wind farm micro‐siting optimisation problem is formulated with the aid of Jensen's wake model. Cabling between the wind turbines and the point of common coupling is an important aspect of the wind farm design as it affects the capital investment as well as income over the lifetime of the wind farm. The cabling layout must satisfy the connection of the wind turbines to the point of common coupling in such a way that the total cable length is reduced while reliability is maintained. Introducing the cabling layout optimisation to the WFLO, further complicates the optimisation problem. An integrated tool is developed to optimise the wind farm layout and cabling simultaneously. The main contribution of this work is the development of an integrated tool that maximizes the energy production of the wind farm via optimal allocation of wind turbines with optimal cable routing. This tool considers the capital cost of wind turbines and cabling, wind farm power production, and power losses in the cabling over the lifetime of the wind farm. The proposed co‐optimisation problem is solved using genetic algorithm. The decision variables are the wind farm layout, cable paths and sizes, and the location of the point of common coupling within the land perimeter. A case study incorporating a multi‐speed and multi‐direction wind profile is carried out to demonstrate the applicability of the proposed approach. Moreover, the proposed methodology is compared to the separate optimisation method where the WFLO and cabling optimisation are solved sequentially with two separate steps. It is shown that the co‐optimisation method is superior in terms of cable power losses, overall wind farm cost, and compactness (land use).
Gravitational instability of solar prominence threads
Authors: Adrover-González A. , Terradas J. , Oliver R. , Carbonell M. 
Published: March 2021

Context. Prominence threads are dense and cold structures lying on curved magnetic fields that can be suspended in the solar atmosphere against gravity.
Aims. The gravitational stability of threads, in the absence of non-ideal effects, is comprehensively investigated in the present work by means of an elementary but effective model.
Methods. Based on purely hydrodynamic equations in one spatial dimension and applying line-tying conditions at the footpoints of the magnetic field lines, we derive analytical expressions for the different feasible equilibria (se) and the corresponding frequencies of oscillation (ω).
Results. We find that the system allows for stable and unstable equilibrium solutions subject to the initial position of the thread (s0), its density contrast (ρt) and length (lt), and the total length of the magnetic field lines (L). The transition between the two types of solutions is produced at specific bifurcation points that have been determined analytically in some particular cases. When the thread is initially at the top of the concave magnetic field, that is at the apex, we find a supercritical pitchfork bifurcation, while for a shifted initial thread position with respect to this point the symmetry is broken and the system is characterised by an S-shaped bifurcation.
Conclusions. The plain results presented in this paper shed new light on the behaviour of threads in curved magnetic fields under the presence of gravity and help to interpret more complex numerical magnetohydrodynamics simulations about similar structures.