bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implication of TYMP genetic variation on the prognosis of patients with colorectal cancer who received capecitabine-based adjuvant chemotherapy after surgical resection: a real-world exploratory study
Authors: Peng Xinyu , Zhang Tao , Sun Junjie , Lin Hengxue , Bai Tianliang , Qiao Yating , Li Yaxin , Li Gang , Li Guicun , Jia Xiongjie , Zhang Aimin 
Published: October 2021

Abstract
Background

Thymidine Phosphorylase (TYMP) gene was of crucial significance in the process of colorectal cancer (CRC) development and also played an important role in capecitabine metabolism. Present study was to identify the association between TYMP polymorphism and prognosis of postoperative patients with CRC who received capecitabine-based adjuvant chemotherapy.
Methods

A total of 218 patients with CRC who were treated with surgical resection and capecitabine-based adjuvant chemotherapy were included in this study retrospectively. Peripheral blood and peripheral blood mononuclear cell (PBMC) specimen of the patients with CRC were collected for the genotyping of TYMP polymorphism and TYMP mRNA expression, respectively. Univariate analysis of genotypes and prognosis was carried out by Kaplan-Meier survival analysis, and multivariate were adjusted by Cox regression analysis. Expression of TYMP according to genotype status was analyzed using non-parameter test.
Results

The median disease-free survival (DFS) of the 218 patients with CRC was 4.6 years, and the median overall survival (OS) was 5.8 years. Regarding the polymorphism analysis, only rs11479 was of clinical significance. The prevalence of rs11479 in TYMP among the 218 patients indicated that minor allele frequency was 0.20 (GG 141 cases, GA 68 cases and AA 9 cases), which accorded with Hardy-Weinberg Equilibrium (P=0.825). Prognostic analysis according to rs11479 genotype status suggested that the median DFS of patients with GG genotype and GA/AA genotype was 3.1 and 6.1 years, respectively (P=0.004). Furthermore, the median OS of patients with GG genotype and GA/AA genotype was 5.0 and 7.0 years, respectively (P=0.033). In order to adjust the confounding factors which might contribute to OS, a multivariate Cox regression analysis was introduced and the results exhibited that rs11479 polymorphism was an independent factor for DFS (HR=1.64, P=0.009). Additionally, of the 65 PBMC specimens, the mRNA expression results indicated that patients with GA/AA genotypes conferred significantly higher mRNA expression of TYMY than that of patients with GG genotype (P<0.001).
Conclusions

TYMP gene polymorphism rs11479 might involve in the prognosis of patients with CRC who received capecitabine based adjuvant chemotherapy through mediation of the mRNA expression of TYMP. Conclusion of present study should be confirmed in prospective clinical trial subsequently.
The Intraosseus Tumor - A Potential Pitfall for the Arthroplasty Surgeon: A Case Report.
Authors: Fromm Julian , Birkenmaier Christof , Goller Sophia , Klein Alexander , Knoesel Thomas , Jansson Volkmar , Dürr Hans Roland 
Published: October 2021

Abstract
Background: Although rare, the annual incidence of intraosseus sarcomas is up to 2 per 100.000 population with a peak in the sixth decade of life. Due to their slow growth and because they are often asymptomatic for a long period of time, they can be misinterpreted as a benign bony lesion. Particularly, if the tumor shows a high myxoid signal on MRI, the lesion might be mistaken for a bone cyst. Case presentation: We present the case of a 70-year-old female complaining of pain in her left knee. Initial imaging showed a grade 3 osteoarthritis, according to the Kellgren-Lawrence classification system, and a cystic lesion of the distal femur. The patient received a total knee endoprothesis and five years after the index surgery, she presented again with pain in her left knee after a fall. Radiographs showed an expansile lesion of the distal femur. A biopsy was performed and the lesion proved to be a chondrosarcoma with myxoid pattern. After en bloc-resection, a megaendoprosthesis was implanted in a palliative approach. Four years later, the patient died without local recurrence due to metastatic disease from an adenocarcinoma of unknown origin. Conclusion: Malignant tumors should be taken into consideration whenever imaging shows an expansile lesion of the bone, even when they are painless, well delimited and slowly or not at all growing. We recommend a biopsy to be performed before performing an arthroplasty in such cases.
Manufacturing of Alumina-Zirconia-CNT Ceramic Composites: A comparison of Conventional and Alumina-Graphite Powder Bath Sintering methods
Authors: Makireddi Diwakar , Ghuge V D , BEHERA ALOK , Puri Y M , Thakare Gajanan , Thawre M M 
Published: October 2021

Abstract
The Zirconia (ZrO2) and Carbon Nano Tubes (CNTs) embedded Alumina (Al2O3) composite fabricated using conventional sintering (CS) possess higher strength, better micro-hardness and more toughness as compared to pure alumina. However, several researchers have reported nonuniform mechanical properties in CS. This non-uniformity can be eliminated using the novel Alumina-Graphite Powder Bath (AGPB) sintering technique. In AGPB, the alumina composites are surrounded by an equal proportion mixture of alumina-graphite powders, providing indirect uniform heating. This converts the heat transfer mode from convectionradiation to conduction and produces uniform mechanical properties across the sintered surface. In this paper, both AGPB and CS techniques were used to fabricate Alumina-ZirconiaCNT (AZC) composites. The uniformity of Physico-mechanical properties was compared for AGPB-AZC and CS-AZC composites. The AGPB-AZC composites exhibited better hardness and minimum pore dispersion as compared to CS-AZC composites. The AGPB-AZC FESEM analysis revealed the formation of single-crystal structures across the sintered surface, which eventually improved the hardness. The mean hardness of the AGPB-AZC (1829.95 HV) composite was enhanced by 43% compared to CS-AZC (1273.5 HV).
A Novel Approach of Modelling Acute Patient Care Across a Healthcare System Using Network Analysis: Effects of The SARS Cov2 Pandemic On Patient Movement
Authors: Kohler Katharina , Jankowski Matthew D , Bashford Thomas , Deepi Goyal , Habermann Elizabeth B , Walker Laura E 
Published: October 2021

Abstract
Background

Consolidation of healthcare in the US has resulted in integrated organizations encompassing large geographic areas, with varying services and complex patient flows. Profound changes in patient volumes and behavior have occurred during the SARS Cov2 pandemic, but understanding these changes across organizations is challenging. Network analysis provides a novel approach to address this.
Methods

We retrospectively evaluated hospital-based encounters with an index emergency department visit in a healthcare system comprising 18 hospitals, using patient transfer as a marker of unmet clinical need. We developed quantitative models of transfers using network analysis incorporating the level of care provided (ward, progressive care, intensive care) during pre-pandemic (May 25, 2018 to March 16, 2020) and mid-pandemic (March 17, 2020 to March 8, 2021) time periods.
Results

829,455 encounters were evaluated. The system functioned as a non-small-world, non-scale-free, dissociative network. Our models reflected transfer destination diversification and variations in volume between the two time points – results of intentional efforts during the pandemic. Known hub-spoke architecture correlated with quantitative analysis.
Conclusion

Applying network analysis in an integrated US healthcare organization demonstrates changing patterns of care and the emergence of bottlenecks in response to the SARS Cov2 pandemic, consistent with clinical experience, providing a degree of face validity. The modeling of multiple influences can identify susceptibility to stress and opportunities to strengthen the system where patient movement is common and voluminous. The technique provides a mechanism to analyze the effects of intentional and contextual changes on system behavior.
Ferroelectric photosensor network: an advanced hardware solution to real-time machine vision
Authors: Cui Boyuan , Fan Zhen , Li Wenjie , Chen Yihong , Dong Shuai , Tan Zhengwei , Cheng Shengliang , Tian Bobo , Tao Ruiqiang , Tian Guo , Chen Deyang , Hou Zhipeng , Qin minghui , Zeng Min , Lu Xubing , Zhou Guofu , Gao Xingsen , Liu Jun-Ming 
Published: October 2021

Abstract
Nowadays the development of machine vision is oriented toward real-time applications such as autonomous driving. This demands a hardware solution with low latency, high energy efficiency, and good reliability. Here, we demonstrate a robust and self-powered in-sensor computing paradigm with a ferroelectric photosensor network (FE-PS-NET). The FE-PS-NET, constituted by ferroelectric photosensors (FE-PSs) with tunable photoresponsivities, is capable of simultaneously capturing and processing images. In each FE-PS, self-powered photovoltaic responses, modulated by remanent polarization of an epitaxial ferroelectric Pb(Zr0.2Ti0.8)O3 layer, show not only multiple nonvolatile levels but also a sign reversibility, enabling the representation of a signed weight in a single device and hence reducing the hardware overhead for network construction. With multiple FE-PSs wired together, the FE-PS-NET acts on its own as an artificial neural network. It is demonstrated that an in situ multiply-accumulate operation between an input image and a stored photoresponsivity matrix is available in our FE-PS-NET hardware. The FE-PS-NET hardware is faultlessly competent for real-time image processing functionalities, including binary pattern classification with an accuracy of 100% and edge detection with an F-Measure of 95.2%. This study highlights the great potential of ferroelectric photovoltaics as the hardware basis of real-time machine vision.
Amyloid-β42 Stimulated Hippocampal Lactate Release is Coupled to Glutamate Uptake
Authors: Hascup Erin R. , Sime Lindsey N. , Peck Mackenzie R. , Hascup Kevin N. 
Published: October 2021

Abstract
Since brain glucose hypometabolism is a feature of Alzheimer’s disease (AD) progression, lactate utilization as an energy source may become critical to maintaining central bioenergetics. We have previously shown that soluble amyloid-β (Aβ)42 stimulates glutamate release through the α7 nicotinic acetylcholine receptor (α7nAChR) and hippocampal glutamate levels are elevated in the APP/PS1 mouse model of AD. Accordingly, we hypothesized that increased glutamate clearance contributes to elevated extracellular lactate levels through activation of the astrocyte neuron lactate shuttle (ANLS). We utilized an enzyme-based microelectrode array (MEA) selective for measuring basal and phasic extracellular hippocampal lactate in male and female C57BL/6J mice. Although basal lactate was similar, transient lactate release varied across hippocampal subregions with the CA1> CA3> dentate for both sexes. Local application of Aβ42 stimulated lactate release throughout the hippocampus of male mice, but was localized to the CA1 of female mice. Coapplication with a nonselective glutamate or lactate transport inhibitor blocked these responses. Expression levels of SLC16A1, lactate dehydrogenase (LDH) A, and B were elevated in female mice which may indicate compensatory mechanisms to upregulate lactate production, transport, and utilization. Enhancement of the ANLS by Aβ42-stimulated glutamate release during AD progression may contribute to bioenergetic dysfunction in AD.
Sub-pixel Spectral Reduction Algorithm for Echelle Spectrometer
Authors: YANG JIN , Yin Lu , SUN CI , WANG MINGJIA , FENG SHULONG , SONG NAN 
Published: October 2021

Abstract
A sub-pixel spectral reduction algorithm for the echelle spectrometer is proposed here to ensure a high spectral resolution for it. The initial model is established by separating cross-dispersion and calculating the imaging offset from the center. The geometric optics formula and mathematical function are used to calculate the offset in two vertical directions respectively. The deviation of the initial model is less than two pixels which is caused by coupling effect of the two vertical directions，and it is too difficult and complicated to calculate true value of the deviation. Therefore, the method of mathematical fitting is used to update the model that the deviation can be reduced. The coordinates were contrast between algorithm and both ray tracing and experimental results to determine the deviation. The results show that the maximum deviation of the whole image plane is not greater than one pixels (sub-pixel level). The proposed spectral reduction algorithm thus significantly improves the spectral accuracy of the echelle spectrometer, and the model is simple to establish and modify, which renders it suitable for applications.
Toxicity Study in a Pig Model of Intraperitoneal Collagenase as an "Enzymatic Scalpel" Directed to Breaks Stroma in Order to Generate a New Perspective for Peritoneal Carcinomatosis Approach. An Experimental Research.
Authors: García-Arranz Mariano , Villarejo-Campos Pedro , Barambio Javier , Gómez-Heras Soledad Garcia , Vega-Clemente Luz , Guadalajara Héctor , Garcia-Olmo Damian 
Published: October 2021

Abstract
Background: The purpose of this study was to evaluate the toxicity using collagenase in peritoneal cavity as a preliminary step to generate a treatment that breaks the stroma surrounds the tumors.Methods: 8 pigs were treated at 2 different collagenase concentrations, previously tested in rats by our groups, with time and temperature control using peritoneal lavage system (PRS System, Combat Medical Ltd) identical to use in human surgeries to treatments through HIPEC, plus 2 pigs were treated with peritoneal lavage only. Blood and peritoneal fluid samples were collected pre-treatment, post-treatment and at 24h after treatment. Histological studies and blood collagenase levels were measured.Results: No complications were observed during the surgeries. Images during treatments showed the release of peritoneal tissue during collagenase washes. After surgery the animals showed no signs of pain. Diet and mobility were normal at 4h. There were no significant differences in hematological and biochemical parameters. MMP1 and MMP2 were quantified in all samples and no differences were found in blood collagenase levels between pre-treatment, post-treatment and 24 h post-treatment.None of collagenase treated animals showed peritoneal adhesions at the second surgery. Histologically, peritoneal organs and serous structures, did not show any microscopic alteration associated with collagenase treatment in any groupsConclusion: Lavage of the peritoneal cavity with doses of up to (100,000 Collagen Digestion Unit/animal for 30 minutes is safe and removes connective tissue from the peritoneal cavity.
The Value of Preoperative Systemic Inflammatory Response index (SIRI) Combined with Albumin-Bilirubin Grading (ALBI) in Predicting Early Recurrence Following Curative Resection of Single Hepatocellular Carcinoma
Authors: Fu Yubin , Feng Zhengkai , Xue Jiahao , Ge Penglei , Li Lin , Wu Yang 
Published: October 2021

Abstract
Background: This study was conducted to explore the risk factors for early recurrence of single hepatocellular carcinoma (HCC) following curative resection, and to evaluate the predictive value of systemic inflammatory response index (SIRI) combined with the albumin - bilirubin index (ALBI) for early recurrence of HCC following curative resection.Methods: We retrospectively analyzed the clinical data of 233 patients with pathologically confirmed HCC who were admitted to the First Affiliated Hospital of Zhengzhou University from January 2015 to January 2018. The optimal cut-off values of alpha-fetoprotein, neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), SIRI, and ALBI were determined by receiver operating characteristic curve (ROC), and the predictive efficiency of each index was compared by area under curve (AUC). A Cox-proportional hazard regression model was used to analyze factors affecting early recurrence. Kaplan-Meier analysis was used to estimate the 2-year recurrence-free survival (RFS) rate of different factors. Results: The AUC of SIRI for predicting early recurrence was 0.644, which was better than that of NLR, MLR, PLR, and SII. SIRI > 0.96, ALBI >-2.7, tumor diameter > 5 cm, and microvascular invasion (MVI) were risk factors for early recurrence after radical resection of HCC. The AUC of SIRI combined with ALBI was 0.759, which was better than that of single SIRI and ALBI. Combined with the above four risk factors, a new prognostic index was constructed, and the AUC for predicting early recurrence was 0.801. Conclusion: SIRI, ALBI, MVI, and tumor diameter could be considered reliable predictors of early recurrence following curative resection of single HCC. Preoperative SIRI combined with ALBI possesses an important reference value in predicting early recurrence following radical resection of HCC. It is helpful for clinicians in identifying high-risk patients with early recurrence, actively carry out postoperative adjuvant treatment, and improving the prognosis of patients.
Mst1 Inhibition As A Cellular Mediator: Prevention Of Anoikis In mBMSCs Through Activating ITGα5β1/FAK Signaling Pathway
Authors: Zhang Tao , Zhang Qian , Wu Shang , Zhang Haizhou , Yu Wancheng 
Published: October 2021

Abstract
BACKGROUND: Lost of adhesion of the mesenchymal stem cell (MSC) to the extracellular matrix (i.e., anoikis) caused poor survival, which limited the therapeutic efficacy of the MSCs. We recently found that the suppression of the mammalian sterile 20-like kinase 1 (Mst1) protected the mouse bone marrow MSCs (mBMSCs) from ROS-mediated cell apoptosis. However, the effect of Mst1 inhibition on the anoikis of mBMSCs remains elusive. METHODS: We propose the use of poly-HEMA-induced anoikis following the Mst1 silencing in the mBMSCs, then cell anoikis were tested by Anoikis Assay Kit and TUNEL Assay Kit, and the change of integrin (ITG)/FAK signal pathway were measured.RESULTS: The mBMSCs were susceptible to anoikis but became anoikis-resistant via Mst1 inhibition. In addition, p- focal adhesion kinase (p-FAK) was upregulated, whereas activated caspase 3 was downregulated in the poly-HEMA-treated mBMSC/sh-Mst1. Mst1 inhibition activated ITGα5 and ITGβ1 but not ITGα4, ITGαv, or ITGβ3. Further analysis revealed that anoikis resistance conferred by sh-Mst1 was disrupted by either inhibiting ITGα5 or ITGβ1 by using siRNA.CONCLUSIONS: Mst1 inhibition resulted in the anoikis resistance of mBMSCs by activating the ITGα5β1/FAK signaling pathway. Inactivation of Mst1 may provide a promising strategy to overcome the anoikis of the implanted MSCs.
A Gene Modular Approach to a Temporal Meningococcal Septic Shock Transcriptome, Enabling Clinical Phenotype Genotype Association
Authors: Rashid Asrar , Uddin Mohammed , Sarpal Amrita , Shaikh M. Guftar , Benakatti Govind , Ismail Javed , Zaki Syed Ahmed , Hussain Amir 
Published: October 2021

Abstract
Meningococcal Septic Shock (MSS) is a life-threatening condition, especially in Infants. Acutely, critical care support to physiologically impact disease progression is an imperative. Exploring the clinical relationship according to temporal microarray data could aid in tracking MSS pathogenesis during this critical period. For the first time, clinical-phenotype gene-expression association studies using Weighted Gene Co Expression Network analysis (WGCNA) was completed on 5 children admitted to the Paediatric Intensive Care Unit (PICU). Then using WGCNA, 18 gene modular clusters were identified. A gene list was generated for each module that was significantly associated with clinical traits. Each gene list underwent gene enrichment analysis. The modules associated with Pediatric Logistic Organ Dysfunction (PELOD) score at 0, 24, and 48 hours showed a changing pattern of enriched pathways related to nuclear (p < 2e-08), cytoplasmic (p < 4e-05) and then extracellular gene regulation (p < 7e-17) for PELOD 0, 24 and 48 hours, respectively. Though WGCNA has been applied to sepsis datasets by other researchers, this study demonstrates association of important temporal clinical characteristics, i.e. PELOD score, with changing cellular processes. This study is an important step in design a gene modular approach with pathophysiological insights, potentially aiding future clinical research design and therapeutic approaches.
Sweat Contamination Induced Surgical Site Infections After Spine Surgery: Three Case Reports and Literature Review
Authors: Ma Yuhang , SHEN KELV , WU DUANRONG , LU ZHENGFENG 
Published: October 2021

Abstract
Background:Surgical site infection (SSI) is one of the most intractable complications following spine surgery during the early postoperative stage. Elderly (age > 70 years), body mass index > 30, smoking, diabetes mellitus, coronary artery disease, chronic obstructive pulmonary disease, anemia, low serum albumin, operation time > 3h, and perioperative blood loss > 500 ml are the common risk factors of SSI after spine surgery. However, there are few published reports about sweat contamination induced surgical site infections with Staphylococcus epidermidis up to date. Staphylococcus epidermidis is a permanent member of the normal human microbiota and has emerged as an important opportunistic pathogen in SSI. We aim to detect the influence of sweat infiltration on SSI with Staphylococcus epidermidis and effective management. Case presentation:A 73-year-old male, a 54-year-old male and a 73-year-old female were admitted to our hospital. All of them underwent posterior compression and fusion surgery with internal fixation and got surgical site infection after primary surgery. Two of them suffered moderate surgical site infection while the third patient with comorbidities suffered severe surgical site infection. Antibiotic therapy and debridement with internal fixation retained were utilized during which microbiological culture were taken. The moderate infection patients got fully recovered after debridement and primary suture while the serious one had recurrence after the first debridement, and then the second operation was performed. SSI, however, relapsed after three days. Vacuum-assisted closure (VAC) system was replaced in the third debridement. The severe patient got well recovered and discharged after displacement of VAC system. Conclusion:Sweat-contaminated is an inducement of SSI with Staphylococcus epidermidis that should attract surgeons’ attention. For mild infection, changing dressing and infrared treatment can achieve good results. For moderate infection, one debridement and primary suture are enough. For severe infection, early application of VAC system can reduce the number of debridement and achieve good clinical outcome.