bluuebunny/crossref_metadata_2025_split huggingface dataset:
Equity at a time of pandemic
Authors: Plamondon Katrina M 
Published: February 2021

Abstract
Health promotion has long aspired for a world where all people can live to their full potential. Yet, COVID-19 illuminates dramatically different consequences for populations bearing heavy burdens of systemic disadvantage within countries and between the Global South and Global North. Many months of pandemic is entrenching inequities that reveal themselves in the vastly differential distribution of hospitalization and mortality, for example, among racialized groups in the USA. Amplified awareness of the intimate relationship between health, social structures, and economy opens a window of opportunity to act on decades of global commitments to prioritize health equity. Choices to act (or not act) are likely to accelerate already vast inequities within and between countries as rapidly as the COVID-19 pandemic itself. Recognizing the inherently global nature of this pandemic, this article explores how determinants of equity are embedded in global responses to it, arguing that these determinants will critically shape our global futures. This article aims to stimulate dialogue about equity-centered health promoting action during a pandemic, using the Canadian Coalition for Global Health Research (CCGHR) Principles for Global Health Research to examine equity considerations at a time of pandemic. Attentiveness to power and the relationship between political economy and health are argued as central to identifying and examining issues of equity. This article invites dialogue about how equity-centered planning, decision-making and action could leverage this massive disruption to society to spark a more hopeful, just, and humane collective future.
Exome sequencing and functional studies in zebrafish identify WDR8 as the causative gene for isolated Microspherophakia in Indian families
Authors: Madhangi M , Dutta Debanjan , Show Sautan , Bhat Vishwanath K , Rather Mohammad I , Tiwari Ankana , Singh Nivedita , Duvvari Maheswara R , Murthy Gowri J , Kumar Arun , Nongthomba Upendra 
Published: February 2021

Abstract
Isolated Microspherophakia (MSP) is an autosomal recessive disorder characterized by a smaller than normal spherical lens. Till date, LTBP2 is the only gene shown to cause MSP. We used homozygosity mapping and whole-exome sequencing and identified a homozygous mutation, c.1148C &gt; T (p.Pro383Leu), in the WDR8 (or WRAP73) gene in two Indian MSP families. In vitro experiments showed that the missense mutation renders the protein unstable. WDR8 is a centriolar protein that has important roles in centrosomal assembly, spindle pole formation and ciliogenesis. Co-immunoprecipitation experiments from HeLa cells indicated that the mutation interferes with the interaction of WDR8 with its binding partners. In zebrafish, both morpholino-mediated knockdown and CRISPR/Cas knockout of wdr8 resulted in decreased eye and lens size. The lack of wdr8 affected cell cycle progression in the retinal cells, causing a reduction in cell numbers in the retina and lens. The reduction in eye size and the cell cycle defects were rescued by exogenous expression of the human wild-type WDR8. However, the human mutant WDR8 (p.Pro383Leu) was unable to rescue the eye defects, indicating that the missense mutation abrogates WDR8 protein function. Thus, our zebrafish results suggested that WDR8 is the causative gene for MSP in these Indian families.
Preoperative risk factors influencing inter-stage mortality after the Norwood procedure
Authors: Ono Masamichi , Kido Takashi , Wallner Marie , Burri Melchior , Lemmer Julia , Ewert Peter , Strbad Martina , Cleuziou Julie , Hager Alfred , Hörer Jürgen 
Published: February 2021

Abstract

OBJECTIVES
With improvements in early survival after the stage I palliation (S1P) Norwood procedure for hypoplastic left heart syndrome (HLHS) and its variants, inter-stage death accounts for an increasing proportion of mortality. Our aim was to identify the risk factors for inter-stage mortality.

METHODS
The records of 322 neonates with HLHS or a variant who underwent the Norwood procedure at our centre between 2001 and 2019 were retrospectively analysed.

RESULTS
The diagnoses included 271 neonates with HLHS (84%) and 51 with variants (16%). Aortic atresia was observed in 138 (43%) patients, mitral atresia in 91 (28%), extracardiac anomalies in 42 (13%) and genetic disorder in 14 (4%). The median age and weight of the patients at the S1P Norwood procedure were 9 (interquartile range: 7–12) days and 3.2 (2.9–3.5) kg, respectively. The median cardiopulmonary bypass time was 137 (107–163) min. Modified Blalock–Taussig shunts were used in 159 (49%) and unvalved right ventricle-to-pulmonary artery shunts in 163 (51%) patients. The number of inter-stage deaths was as follows: between S1P and stage II palliation (S2P), 61 including 38 early (&lt;30 days) and 23 late (&gt;30 days) deaths, and between S2P and stage III palliation, 32 deaths. Low birth weight (&lt;2.5 kg) (odds ratio 4.37, P = 0.020) and restrictive atrial septum (odds ratio 2.97, P = 0.013) were identified as risks for early mortality. Low birth weight [hazard ratio (HR) 0.99/g, P = 0.002] was a risk for inter-stage mortality between S1P and S2P. Extracardiac anomalies (HR 4.75, P = 0.049) and significant pre-S1P atrioventricular valve regurgitation (HR: 7.72, P = 0.016) were risks for inter-stage mortality between S2P and stage III palliation. Other anatomical variables including aortic atresia, anatomical subtypes and the diameter of the ascending aorta nor shunt type were not identified as risk factors for mortality during any inter-stage period.

CONCLUSIONS
The risk factors for inter-stage attrition after the Norwood procedure were different between each stage. Preoperative factors, including birth weight, restrictive atrial septum and extracardiac anomalies, adversely affected the inter-stage mortality.
Technical note: validation of the GreenFeed system for measuring enteric gas emissions from cattle
Authors: McGinn Sean M , Coulombe Jean-Franҫois , Beauchemin Karen A 
Published: February 2021

Abstract
There are knowledge gaps in animal agriculture on how to best mitigate greenhouse gas emissions while maintaining animal productivity. One reason for these gaps is the uncertainties associated with methods used to derive emission rates. This study compared emission rates of methane (CH4) and carbon dioxide (CO2) measured by a commercially available GreenFeed (GF) system with those from (1) a mass flow controller (MFC) that released known quantities of gas over time (i.e., emission rate) and (2) a respiration chamber (RC). The GF and MFC differed by only 1% for CH4 (P = 0.726) and 3% for CO2 (P = 0.013). The difference between the GF and RC was 1% (P = 0.019) for CH4 and 2% for CO2 (P = 0.007). Further investigation revealed that the difference in emission rate for CO2 was due to a small systematic offset error indicating a correction factor could be applied. We conclude that the GF system accurately estimated enteric CH4 and CO2 emission rates of cattle over a short measurement period, but additional factors would need to be considered in determining the 24-hr emission rate of an animal.
Optimization of a Diet for the Greater Wax Moth (Lepidoptera: Pyralidae) Using Full Factorial and Mixture Design
Authors: Hickin Mauri , Nadel Hannah , Schal Coby , Cohen Allen C 
Published: February 2021

Abstract
Diet optimization is an important process to increase the efficiency of rearing insects and can be used to develop high-quality insects with specific fitness and life-history traits. Galleria mellonella (L.), the greater wax moth, is widely used in research, microbiology assays, as pet food, and host for biological control agents. Although artificial diets for G. mellonella have been researched and optimized for decades, preliminary tests indicated that the predominantly utilized G. mellonella diet could be improved to yield larger larvae with a short development time. We used a design of experiments (DOE) approach that incorporated multiple full factorial designs and a final mixture design to test the qualitative and quantitative effects of ingredients and their interactions on larval mass and survival. Analysis of 17 ingredient variations in 35 diet formulations yielded an optimized diet that supported high survival and 2.4-fold greater larval body mass than the standard rearing diet. This study demonstrates the importance and efficiency of statistical DOE in guiding the optimization of insect diets to improve traits that represent the quality and fitness of the reared insects.
Susceptibility of Brazilian Populations of Anastrepha fraterculus, Ceratitis capitata (Diptera: Tephritidae), and Drosophila suzukii (Diptera: Drosophilidae) to Selected Insecticides
Authors: Morais Maíra C , Rakes Matheus , Padilha Aline C , Grützmacher Anderson D , Nava Dori E , Bernardi Oderlei , Bernardi Daniel 
Published: February 2021

Abstract
Anastrepha fraterculus (Wiedemann), Ceratitis capitata (Wiedemann), and Drosophila suzukii (Matsumura) are the main fruit pests in Brazil. Here, we evaluated the susceptibility of Brazilian populations of A. fraterculus, C. capitata, and D. suzukii to selected insecticides. In ingestion bioassays, adults from a laboratory susceptible population of each species were exposed to five different modes of action of insecticide. Then, field populations of each species were exposed to the diagnostic concentrations to evaluate possible changes in susceptibility. Our findings indicate that lambda-cyhalothrin, malathion, and spinosad had similar levels of toxicity against a susceptible laboratory population of A. fraterculus, with LC50 values of 6.34, 6.54, and 8.76 µg a.i./ml, respectively. Ceratitis capitata had similar susceptibilities to spinosad (1.30 µg a.i./ml), spinetoram (2.76 µg a.i./ml), and malathion (7.10 µg a.i./ml), but a lower susceptibility to lambda-cyhalothrin (76.55 µg a.i./ml). For D. suzukii, the LC50 values of deltamethrin (0.67 µg a.i./ml), malathion (3.30 µg a.i./mL), spinosad (4.16 µg a.i./ml), and spinetoram (4.75 µg a.i./ml) were lower than for abamectin (15.02 µg a.i./ml), acetamiprid (39.38 a.i./ml), and thiamethoxam (70.15 µg a.i./ml). The diagnostic concentrations, based on LC99 values of the insecticides, caused more than 99% mortality for most field populations of each species. For lambda-cyhalothrin the populations RS-1 (A. fraterculus) and SC (C. capitata) showed approximately 10% of live insects. These differences represent the natural variation in population susceptibility and not due to insecticide selection pressure. The diagnostic concentrations defined here should be used in future resistance monitoring programs in Brazil.
Implications of the germline variants of DNA damage response genes detected by cancer precision medicine for radiological risk communication and cancer therapy decisions
Authors: Hosoya Noriko , Miyagawa Kiyoshi 
Published: February 2021

ABSTRACT
Large-scale cancer-associated gene testing is now being rapidly incorporated into clinical settings, and is leading to incidental identification of the germline variants present in cancer patients. Because many cancer susceptibility genes are related to DNA damage response and repair, the variants may reflect not only the susceptibility to cancer but also the genetically defined radiation sensitivity of the patients and their relatives. When the presence of a certain germline variant increases the risk for developing radiation toxicity or radiation-induced secondary cancers, it will greatly influence the clinical decision-making. In order to achieve optimal radiological risk communication and to select the best cancer management for a given patient based on information from gene testing, healthcare professionals including genetic counselors, risk communicators and clinicians need to increase their knowledge of the health effects of various genetic variants. While germline loss-of-function mutations in both of the alleles of the DNA damage response genes cause rare hereditary diseases characterized by extreme hypersensitivity to radiation, the health effects of the carriers who have germline variants in one allele of such genes would be a matter of debate, especially when the significance of the variants is currently unknown. In this review, we describe the clinical significance of the genetic variants of the important DNA damage response genes, including ATM and TP53, and discuss how we can apply current knowledge to the management of cancer patients and their relatives from a radiological point of view.
Testing the turbulent origin of the stellar initial mass function
Authors: Nam Donghee G , Federrath Christoph , Krumholz Mark R 
Published: February 2021

ABSTRACT
Supersonic turbulence in the interstellar medium (ISM) is closely linked to the formation of stars; hence, many theories connect the stellar initial mass function (IMF) with the turbulent properties of molecular clouds. Here, we test three turbulence-based IMF models (by Padoan and Nordlund, Hennebelle and Chabrier, and Hopkins) that predict the relation between the high-mass slope (Γ) of the IMF, dN/d log M ∝ MΓ, and the exponent n of the velocity power spectrum of turbulence, Ev(k) ∝ k−n, where n ≈ 2 corresponds to typical ISM turbulence. Using hydrodynamic simulations, we drive turbulence with an unusual index of n ≈ 1, measure Γ, and compare the results with n ≈ 2. We find that reducing n from 2 to 1 primarily changes the high-mass region of the IMF (beyond the median mass), where we measure high-mass slopes within the 95 per cent confidence interval of −1.5 &lt; Γ &lt; −1 for n ≈ 1 and −3.7 &lt; Γ &lt; −2.4 for n ≈ 2, respectively. Thus, we find that n = 1 results in a significantly flatter high-mass slope of the IMF, with more massive stars formed than for n ≈ 2. We compare these simulations with the predictions of the three IMF theories. We find that while the theory by Padoan and Nordlund matches our simulations with fair accuracy, the other theories either fail to reproduce the main qualitative outcome of the simulations or require some modifications. We conclude that turbulence plays a key role in shaping the IMF, with a shallower turbulence power spectrum producing a shallower high-mass IMF, and hence more massive stars.
Infrared emission of z ∼ 6 galaxies: AGN imprints
Authors: Di Mascia F , Gallerani S , Behrens C , Pallottini A , Carniani S , Ferrara A , Barai P , Vito F , Zana T 
Published: February 2021

ABSTRACT
We investigate the infrared (IR) emission of high-redshift (z ∼ 6), highly star-forming (${{\rm SFR}\gt 100\,{\rm M}_{\odot }\, {\rm yr}^{-1}}$) galaxies, with/without active galactic nuclei (AGN), using a suite of cosmological simulations featuring dust radiative transfer. Synthetic spectral energy distributions (SEDs) are used to quantify the relative contribution of stars/AGN to dust heating. In dusty (Md ≳ 3 × 107 M⊙) galaxies, ≳50–90 per cent of the ultraviolet (UV) radiation is obscured by dust inhomogeneities on scales ≳100 pc. In runs with AGN, a clumpy, warm (≈250 K) dust component coexists with a colder (≈60 K) and more diffuse one, heated by stars. Warm dust provides up to ${50 {{\ \rm per\ cent}}}$ of the total infrared (IR) luminosity, but only ${\lesssim}0.1 {{\ \rm per\ cent}}$ of the total mass content. The AGN boosts the MIR flux by 10–100 times with respect to star-forming galaxies, without significantly affecting the far-IR. Our simulations successfully reproduce the observed SED of bright (MUV ∼ −26) z ∼ 6 quasars, and show that these objects are part of complex, dust-rich merging systems, containing multiple sources (accreting black holes and/or star-forming galaxies) in agreement with recent HST and ALMA observations. Our results show that the proposed ORIGINS missions will be able to investigate the mid-IR (MIR) properties of dusty star-forming galaxies and to obtain good-quality spectra of bright quasars at z ∼ 6. Finally, the MIR-to-FIR flux ratio of faint (MUV ∼ −24) AGN is &gt;10 times higher than for normal star-forming galaxies. This implies that combined JWST/ORIGINS/ALMA observations will be crucial to identify faint and/or dust-obscured AGN in the distant Universe.
KERA: analysis tool for multi-process, multi-state single-molecule data
Authors: Tibbs Joseph , Ghoneim Mohamed , Caldwell Colleen C , Buzynski Troy , Bowie Wayne , Boehm Elizabeth M , Washington M Todd , Tabei S M Ali , Spies Maria 
Published: February 2021

Abstract
Molecular machines within cells dynamically assemble, disassemble and reorganize. Molecular interactions between their components can be observed at the single-molecule level and quantified using colocalization single-molecule spectroscopy, in which individual labeled molecules are seen transiently associating with a surface-tethered partner, or other total internal reflection fluorescence microscopy approaches in which the interactions elicit changes in fluorescence in the labeled surface-tethered partner. When multiple interacting partners can form ternary, quaternary and higher order complexes, the types of spatial and temporal organization of these complexes can be deduced from the order of appearance and reorganization of the components. Time evolution of complex architectures can be followed by changes in the fluorescence behavior in multiple channels. Here, we describe the kinetic event resolving algorithm (KERA), a software tool for organizing and sorting the discretized fluorescent trajectories from a range of single-molecule experiments. KERA organizes the data in groups by transition patterns, and displays exhaustive dwell time data for each interaction sequence. Enumerating and quantifying sequences of molecular interactions provides important information regarding the underlying mechanism of the assembly, dynamics and architecture of the macromolecular complexes. We demonstrate KERA’s utility by analyzing conformational dynamics of two DNA binding proteins: replication protein A and xeroderma pigmentosum complementation group D helicase.
Inpatient palliative care utilization for patients with brain metastases
Authors: Price Meghan , Howell Elizabeth P , Dalton Tara , Ramirez Luis , Howell Claire , Williamson Theresa , Fecci Peter E , Anders Carey K , Check Devon K , Kamal Arif H , Goodwin C Rory 
Published: February 2021

Abstract

Introduction
Given the high symptom burden and complex clinical decision making associated with a diagnosis of brain metastases (BM), specialty palliative care (PC) can meaningfully improve patient quality of life. However, no prior study has formally evaluated patient-specific factors associated with PC consultation among BM patients.

Methods
We examined the rates of PC consults in a cohort of 1303 patients with BM admitted to three tertiary medical centers from October 2015 to December 2018. Patient demographics, surgical status, 30-day readmission, and death data were collected via retrospective chart review. PC utilization was assessed by identifying encounters for which an inpatient consult to PC was placed. Statistical analyses were performed to compare characteristics and outcomes between patients who did and did not receive PC consults.

Results
We analyzed 1303 patients admitted to the hospital with BM. The average overall rate of inpatient PC consultation was 19.6%. Rates of PC utilization differed significantly by patient race (17.5% in White/Caucasian vs 26.0% in Black/African American patients, P = .0014). Patients who received surgery during their admission had significantly lower rates of PC consultation (3.9% vs 22.4%, P &lt; .0001). Patients who either died during their admission or were discharged to hospice had significantly higher rates of PC than those who were discharged home or to rehabilitation (P &lt; .0001).

Conclusions
In our dataset, PC consultation rates varied by patient demographic, surgical status, discharging service, and practice setting. Further work is needed to identify the specific barriers to optimally utilizing specialty PC in this population.
The complexities of approaching nutrition in inflammatory bowel disease: current recommendations and future directions
Authors: Russell Lindsey A , Balart Maria Teresa , Serrano Pablo , Armstrong David , Pinto-Sanchez Maria Ines 
Published: February 2021

Abstract
Inflammatory bowel diseases (IBDs), including Crohn's disease and ulcerative colitis predispose patients to malnutrition due to a combination of increased basal metabolic rate, decreased oral intake, and increased nutritional losses and malabsorption. Malnutrition is common, affecting up to 75% of patients with Crohn's disease and 62% of patients with ulcerative colitis, and is associated with worse disease prognosis, higher complication rates, decreased quality of life, and increased mortality risk. It is imperative to screen patients with IBD for malnutrition to assess those at increased risk and treat accordingly to prevent progression and complications. This literature review provides an overall approach to optimizing nutrition in IBD, focusing on the assessment for the diagnosis of malnutrition, management of macro- and micronutrient deficiencies, and identification of areas for future study.
Seven computations of the social brain
Authors: Molapour Tanaz , Hagan Cindy C , Silston Brian , Wu Haiyan , Ramstead Maxwell , Friston Karl , Mobbs Dean 
Published: February 2021

AbstractThe social environment presents the human brain with the most complex information processing demands. The computations that the brain must perform occur in parallel, combine social and nonsocial cues, produce verbal and nonverbal signals and involve multiple cognitive systems, including memory, attention, emotion and learning. This occurs dynamically and at timescales ranging from milliseconds to years. Here, we propose that during social interactions, seven core operations interact to underwrite coherent social functioning; these operations accumulate evidence efficiently—from multiple modalities—when inferring what to do next. We deconstruct the social brain and outline the key components entailed for successful human–social interaction. These include (i) social perception; (ii) social inferences, such as mentalizing; (iii) social learning; (iv) social signaling through verbal and nonverbal cues; (v) social drives (e.g. how to increase one’s status); (vi) determining the social identity of agents, including oneself and (vii) minimizing uncertainty within the current social context by integrating sensory signals and inferences. We argue that while it is important to examine these distinct aspects of social inference, to understand the true nature of the human social brain, we must also explain how the brain integrates information from the social world.