bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection
Authors: Liu Yuan , Li Taotao , Yang Gaojian , Deng Yan , Mou Xianbo , He Nongyue 
Published: March 2021

Abstract
Background
Chlorpyrifos (Chl) is an organophosphorus pesticide, which has toxicity to environment, animals and human beings. To overcome the shortages of traditional detection methods of small molecular, this study aimed to develop a sensitive, simple, low cost and on-site rapid method for Chl analysis and detection. Thus, we developed a simple label-free gold nanoparticles (AuNPs) based colorimetric biosensor aptasensor for Chl detection using an aptamer as the caputure probe.
Results
The Chl-aptamer with low dissociation constant (Kd) of 58.59 ± 6.08 (nM) was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the limit of detection as low as 14.46 nM. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, which showed satisfying results with excellent recovery values between 96.2% and 105.6% and acceptable RSD values below 5%.
Conclusions
The developed colorimetric aptasensor can serve as a promising candidate for Chl detection in the area of biosensors, which also showed a great potential in simple, cheap and rapid detection of Chl.
Will You Come Back to Contribute? Investigating the Inactivity of OSS Core Developers in GitHub
Authors: Calefato Fabio , Gerosa Marco Aurelio , Iaffaldano Giuseppe , Lanubile Filippo , Steinmacher Igor Fabio 
Published: March 2021

Abstract
Several Open-Source Software (OSS) projects depend on the continuity of their development communities to remain sustainable. Understanding how developers become inactive or why they take breaks can help communities prevent abandonment and incentivize developers to come back. In this paper, we propose a novel method to identify developers’ inactive periods by analyzing the individual rhythm of contributions to the projects. Using this method, we quantitatively analyze the inactivity of core developers in 18 OSS organizations hosted on GitHub. We also survey core developers to receive their feedback about the identified breaks and transitions. Our results show that our method was effective for identifying developers’ breaks. About 94% of the surveyed core developers agreed with our state model of inactivity; 71% and 79% of them acknowledged their breaks and state transition, respectively. We also show that all core developers take breaks (at least once) and about a half of them (~ 45%) have completely disengaged from a project for at least one year. We also analyzed the probability of transitions to/from inactivity and found that developers who pause their activity have a ~ 35 to ~ 55% chance to return to an active state; yet, if the break lasts for a year or longer, then the probability of resuming activities drops to ~ 21–26%, with a ~ 54% chance of complete disengagement. These results may support the creation of policies and mechanisms to make OSS community managers aware of breaks and potential project abandonment.
Shorebird’s Longer Migratory Distances are Associated with Larger ADCYAP1 Microsatellites and Greater Morphological Complexity of Hippocampal Astrocytes
Authors: Miranda Diego de Almeida , Silva Juliana Araripe Gomes da , Magalhães Nara Gyzely de Gyzely , Siqueira Lucas Silva de , Abreu Cintya Castro de , Pereira Patrick Douglas Corrêa , Henrique Ediely Pereira , Chira Pedro Arthur Campos da Silva , Melo Mauro André Damasceno de , Rêgo Péricles Sena do , Diniz Daniel Guerreiro , Sherry David Francis , Diniz Cristovam Wanderley Picanço , Diniz Cristovam Guerreiro 
Published: March 2021

Abstract
Background

For the epic journey of autumn migration, long-distance migratory birds use innate and learned information and follow strict schedules imposed by genetic and epigenetic mechanisms, the details of which remain largely unknown. In addition, bird migration requires integrated action of different multisensory systems for learning and memory, and the hippocampus appears to be the integration center for this task. In previous studies we found that contrasting long-distance migratory flights differentially affected the morphological complexity of two types of hippocampus astrocytes. Recently, a significant association was found between the latitude of the reproductive site and the size of the ADCYAP1 allele in long distance migratory birds.
Methods

We tested for correlations between astrocyte morphological complexity, migratory distances, and size of the ADCYAP1 allele in three long-distance migrant species of shorebird and one non-migrant.
Results

Significant differences among species were found in the number and morphological complexity of the astrocytes, as well as in the size of the microsatellites of the ADCYAP1 gene. We found significant associations between the size of the ADCYAP1 microsatellites, the migratory distances, and the degree of morphological complexity of the astrocytes.
Conclusion

We suggest that associations between astrocyte number and morphological complexity, ADCYAP1 microsatellite size, and migratory behavior may be part of the adaptive response to the migratory process of shorebirds.
Evaluation of low to Moderate Arsenic Exposure, Metabolism and Skin Lesions in a Turkish Rural Population
Authors: Alshana Usama , Altun Beril , Ertaş Nusret , Çakmak Gonca , Kadioglu Ela , Hisarlı Deniz , Aşık Elif , Atabey Eşref , Bora Selin , Ataman O. Yavuz , Çelebi Celalettin Rumi , Bilir Nazmi , Serçe Hakan , Tuncer A. Murat , Burgaz Sema 
Published: March 2021

Abstract
Background: There is no human data regarding exposure, metabolism and potential health effects of arsenic (As) contamination in drinking water in the Central Anatolian region, Turkey. Methods: Residents in the 10 villages with drinking water total As (T-As) level >50 µg/L and 10-50 µg/L were selected as an exposed group (n=420) and, <10 µg/L as a control group (n=185). Time-weighted average-As (TWA-As) intakes were calculated from T-As analysis of drinking water samples (DWS). Other metals in DWS, concentrations of T-As in urine and hair samples, urinary As species (S-As) (As (III), As (V), MMA-V and DMA-V), and some micronutrients in serum samples of residents were analyzed. Primary and secondary methylation indexes (PMI, SMI; respectively) were assessed from S-As concentrations. . Skin lesion presence was examined. Results: TWA-As intake was 75.15 ppb in exposed group. Urinary and hair T-As and urinary S-As concentrations were significantly higher in exposed group (p<0.05). PMI and SMI values are revealed that methylation capacities of the residents were efficient and there was no saturation in As metabolism. No significant increase was observed in the frequency of skin lesions (hyperpigmentation, hypopigmentation, keratosis) of exposed group (p>0.05). Only frequency of keratosis either at hand or foot was higher in individuals with hair As concentration >1 µg/g (p<0.05).Conclusion: Current study findings of chronic As exposure through drinking water of individuals living in the study area, could contribute to overall health risk assessment of regulatory agencies’ meta-analysis efforts on low-to moderate As exposure (<100 µg/L).
Changes In The Macular Vascular Density after Bariatric Surgery Measured by Optical Coherence Tomography Angiography
Authors: ElShazly Malak I , Salama Marwa M , Elessawy Kareem 
Published: March 2021

Abstract
Purpose: To evaluate the effect of the weight loss, 3 months after bariatric surgery on the macular thickness and macular vascular density by optical coherence tomography angiography (OCTA).Methods: Forty obese patients were included in this prospective study. Body mass index (BMI), macular thickness (whole, fovea, parafovea and perifovea), macular vascular density (VD) in superficial capillary plexus (whole, fovea, parafovea and perifovea), and macular vascular density in deep capillary plexus (whole, fovea, parafovea and perifovea) were measured before and 3 months after bariatric surgery.Results: The BMI was significantly reduced postoperatively to 43.75±4.4 kg/m2 compared to the preoperative results 55.31±5.1 kg/m2 (p<0.0001). There was significant increase in the macular thickness in the fovea and parafovea postoperatively (p <0.001), but was not significant in the perifovea. There was significant increase in the macular vascular density in the deep capillary plexus postoperatively (p<0.05), but, there was no significant increase in the macular vascular density in the superficial capillary plexus postoperatively (p = 0.4). Significant correlations were detected between the BMI changes and changes in different macular parameters.Conclusion: Bariatric surgery showed significant effect on certain indices in the macular thickness and macular vascular density especially in the deep capillary plexus. Therefore, OCTA is considered a valuable tool to assess the short term changes in the macular microcirculation following significant weight reduction.
Ethyl acetate extracts of Bungeanum ameliorates cognitive deficits by suppressing NLRP3 inflammasome-dependent pyroptosis in aging mice
Authors: Zhao Meihuan , Dai Yuan , Li Ping , Wang Jie , Ma Tengyun , Xu Shijun 
Published: March 2021

Abstract
Background: The NLRP3-mediated pyroptosis is a key player in the development of the age-associated neurodegenerative disease. Zanthoxylum bungeanum Maxim (Rutaceae), a homologous of medicine and foodstuff, has previously been demonstrated the potential prevention of cognitive dysfunction in aging mice. However, it is still unknown which fraction is the material basis responsible for their therapeutic effects and whether Z. bungeanum could confer anti-cognitive deficits activity via restraining NLRP3-mediated pyroptosis. Thus, in the current study, we explored the active fraction of Z. bungeanum against cognitive deficits and its underlying mechanism.Methods: In the present study, the D-galactose-induced mouse model of aging was established to explore the effect of cognitive impairment of four fractions of Z. bungeanum, including petroleum ether (PE), methylene chloride (DCM), ethyl acetate (EA), and n-butanol (N-BAI). We next activated the NLRP3 inflammasome in BV-2 microglia cells to investigate the mechanisms for the neuroprotective effect of the active fraction of Z. bungeanum.Results: We demonstrated that the mice treated with EA had significantly alleviated the memory deficit induced by D-galactose. Meanwhile, EA up-regulated the cortex NeuN protein level, improved the survival and morphology of hippocampal neurons. We further found that EA significantly alleviated oxidative damage, inhibited activation of microglia, and suppressed NLRP3 inflammasome activation and subsequent cleaved Caspase-1, IL-1β, IL-18, and GSDMD-N. Correspondingly, in vitro data showed that EA protected BV-2 cells against Lipopolysaccharide (LPS) and adenosine triphosphate (ATP) elicited NLRP3 inflammasome activation and pyroptosis, evidenced by declined the protein levels of NLRP3, cleaved Caspase-1, IL-1β, IL-18, and GSDMD-N. These data indicated that EA inhibited NLRP3-mediated pyroptosis.Conclusions: Our work revealed that the EA active fraction of Z. bungeanum protected neurons from inflammation through inhibition of NLRP3 and subsequent pyroptosis in microglia, and suggested promising clinical use of EA for age-associated neurodegenerative disease.
A Pilot Study Investigating the Safety and Feasibility of Endoscopic Dilation Using a Radial Incision and Cutting Technique for Benign Strictures of the Small Intestine: A Study Protocol
Authors: Moroi Rintaro , Shiga Hisashi , Nochioka Kotaro , Shimoyama Yusuke , Kuroha Masatake , Kakuta Yoichi , Kinouchi Yoshitaka , Masamune Atsushi 
Published: March 2021

Abstract
BackgroundSmall benign intestinal stenosis is usually treated by endoscopic balloon dilation (EBD) or surgery. Although, EBD and surgery are able to resolve the stenosis in most case, they are associated with several problems such as insufficient dilation and surgical stress, respectively. On the contrary, a novel approach called radial incision and cutting (RIC) is reported to have several benefits when compared to EBD and surgery. We can currently adopt RIC only for the strictures in the colon or terminal ileum and not for those stenotic lesions present further in the small intestine where balloon-assisted endoscopy is utilized, because long-type electric knife is currently not approved for use in Japan. We will herein conduct a pilot study to investigate the safety and feasibility of RIC for treating the benign stenoses of the small intestine using the long-type electric knife.Methods This will be a single-center, single-arm, interventional trial. The major criteria for inclusion will be age ranging from 20 to 80 years and the presence of benign stenosis in the small intestine. We will perform RIC in 10 participants. The primary outcome is the safety of this procedure, which will be assessed by measuring the frequency of adverse events of special interest. The secondary outcomes will be technical success rate, improvement in subjective symptoms, procedure time, and duration of hospitalization.DiscussionThis pilot study will provide useful information that will aid in adopting RIC for treating the benign strictures present in the small intestine. Trial registration jRCT Identifier, jRCTs022200040. Registered on 1 March 2021.URL: https://jrct.niph.go.jp/re/reports/detail/11533
PADI1 contributes to epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma through activating ERK1/2-p38 signaling pathway
Authors: Ji Tengfei , Ma Keqiang , Chen Liang , Cao Tiansheng 
Published: March 2021

Abstract
Background

Peptidylarginine deiminase 1 (PADI1) may be relative with the progression of epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PAAD). We aim to explore the role of PADI1 in PAAD.
Methods

The expression pattern of PADI1 in PAAD tissues and normal tissues was analyzed using The Cancer Genome Atlas (TCGA) dataset. PADI1 was knocked down in CFPAN-1 and HPAC cells, while overexpressed in PANC-1 and Bxpc-3 cells by RNA interference. Wound healing assay was performed to analyze relative cell migration distance. Cell migration and invasion were assessed by Transwell assay. Related protein expression levels were measured by western blot and immunofluorescence.
Results

Bioinformatics analysis showed that PADI1 was overexpressed in PAAD tissues and associated with worse survival prognosis. Knockdown of PADI1 suppressed the cell migration, invasion and activated ERK1/2-p38 signaling pathway in CFPAN-1 and HPAC cells. Overexpression of PADI1 obtained the opposite results in PANC-1 and Bxpc-3 cells. Moreover, treatment with MEK1/2 inhibitor significantly recovered the effects of PADI1 knockdown on cell migration, invasion, EMT process and p-ERK1/2 and p38 expression in CFPAN-1 and HPAC cells.
Conclusions

Our data suggested that PADI1 may function as an oncogene in regulating metastasis in vitro in PAAD.
Microwave heating oxidative stabilization of KMnO4 modified polyacrylonitrile-based carbon fiber precursor
Authors: Zhang Cheng , Liu Jianhua 
Published: March 2021

Abstract
Dielectric property, bulk density, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), wide angle X-ray diffraction (WAXD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and mechanical properties were analyzed. PAN fibers (PFs) are polar materials, and the dielectric constant of KMnO4 modification fibers (Mn-PFs) is reduced. The bulk density of Mn-PFs under microwave stabilization (Mn-MSFs) is 0.04 g/cm3 higher than that of the microwave stabilized fibers (MSFs), shortening the value in one temperature zone. Compared with MSFs5, the stability of Mn-MSFs5 is improved by 10%, and the surface O content and O-containing functional groups are improved. The (002) crystal plane diffraction peak of Mn-MSFs5 is higher than that of MSFs5, and it has a higher stabilized structure. Moreover, after stabilization, the Mn element does not exist on the fiber surface and does not affect the structure of the stabilized fiber. The fineness of Mn-MSFs5 is 1.07 dtex, tensile strength is 1.52 cN/dtex, tensile modulus is 59.3 cN/dtex and elongation at break is 13.5%. It has excellent mechanical properties. In addition, a structural transformation of the thermal stabilization process is proposed, that is, the cyclization reaction first occurs, and then the dehydrogenation and oxidation reactions are performed.
A Study on the Effect of Personality Traits and Social Support on Psychological Health of Chinese College Students Based on Data Mining Technology
Authors: Wu Ying , Guo Huaqing , Li Hongxia , Yang Wenxiao 
Published: March 2021

Abstract
Background

With the continuous development of the society, the pace of life is accelerating, psychological health has become an important factor affecting people's quality of life. Domestic studies have shown that mental diseases have become an important cause of College Students' sick leave, withdrawal, addiction, crime and suicide. Personality traits and social support factors have been considered to be related to psychological health.
Methods

In this study, Symptom Checklist-90 (SCL-90), Eysenck Personality Questionaire (EPQ) and Perceived Social Support Scale (PSSS) of some freshmen in 2020 were randomly selected from the psychological census database of a university in China as the research data. The decision tree algorithm was used to establish a predictive model for psychological health related personality straits and social support factors of college students. In association analysis, we got the most closely effect factors with psychological health or psychological abnormality by analyzing important association rules. According to the distance, all effect factors were classified into different clusters by system clustering, and the relationship between effect factors and mental health was further analyzed.
Results

Combining the results of the three algorithms, we found that social support and personality traits factors had a certain rule on the effect of college students' psychological health, and social support factors were more important than personality traits factors. Among the social support factors, the most important was family support, followed by friend support and other support. The higher the level of support of the three, the greater the possibility of psychological health. In the personality traits factors, the most important was N, followed by E, and P. The three grades were high or low, may lead to abnormal psychology, and the medium grade was an important condition to maintain psychological health.
Conclusion

The results provided important references for the analysis of the effect factors on psychological health and important theoretical basis for the formulation of psychological intervention measures for college students.
Association of global sagittal deformity with functional disability two years after total hip arthroplasty
Authors: Okamoto Yoshinori , Wakama Hitoshi , Okayoshi Tomohiro , Otsuki Shuhei , Neo Masashi 
Published: March 2021

Abstract
Introduction: The relationship between spinopelvic alignment and functional disability after total hip arthroplasty (THA) has not been fully elucidated despite the growing recognition of its importance on patient-reported outcome measures. We aimed to determine whether global sagittal deformity was associated with post-operative disability.Materials and methods: This prospective analysis was based on 208 THAs that were followed up for 2 years. The Hip Disability and Osteoarthritis Outcome Score-Joint Replacement (HOOS-JR) ranging from a scale of 0 (complete joint disability) to 100 (perfect joint health) was utilised to divide eligible patients into two groups with and without disability, using 70 as the threshold. Multivariate analysis was performed to evaluate the factors associated with disability. To identify the cut-off value of the parameters for predicting disability (HOOS-JR <70/100), we used the receiver-operating characteristic curve.Results: The disability (30 hips) and control (178 hips) groups showed a significant difference in body height (p = 0.020), pre-operative T1 pelvic angle divided by pelvic incidence (T1PA/PI) (p = 0.018), pelvic incidence minus lumbar lordosis (p = 0.027), post-operative HOOS-JR (p = 0.010), and satisfaction (p = 0.033). On multivariate analysis, the following factors were associated with persistent disability: T1PA/PI >0.2 (odds ratio [OR], 2.11; 95% confidence interval [CI], 1.19–4.14; p < 0.001) and height <148 cm (OR, 1.26; 95% CI, 1.09–1.48; p = 0.011). The cut-off value of pre-operative T1PA/PI was >0.19 with a sensitivity of 95% and specificity of 85%. Post-operative satisfaction (p < 0.001) and HOOS-JR (p = 0.023) differed between the two groups when the pre-operative cut-off value was chosen as 0.2.Conclusions: A T1PA/PI >0.2 was associated with greater disability after THA. Clinicians should be aware that patient-related factors, including global spinal deformities, particularly in patients with a short stature, can influence THA outcomes at 2 years postoperatively.
Endovascular Retrieval of a Dislocated Pushable Coil in the Common Hepatic Artery Using a Cerebral Stent Retriever
Authors: Shamseldin Mohammed 
Published: March 2021

Abstract
BACKGROUNDThis is case of removing a dislocated pushable coil from the common hepatic artery (CHA) as a possible complication of using pushable coils in the embolization of an upper gastrointestinal bleeding (UGIB) from the gastroduodenal artery (GDA) by using a pRESET stent retriever (Phenox, Bochum, Germany) which is utilized mainly for treatment of endovascular stroke.CASE PRESENTATIONAn 88-year-old female patient was referred to our hospital to get an emergency embolization of the GDA causing an UGIB with a relevant drop of the hemoglobin level. During the routine embolization of the GDA using pushable coils, a complete dislocation of the last coil into the CHA took place leading to a relevant slowing down of the arterial blood flow to the liver. A decision was thereby made to remove the dislocated coil to avoid further possible complications which was successfully achieved. CONCLUSIONSVarious stent retrievers have been proven to be effective in removing dislocated coils during intracerebral coiling of different pathologies. This case report is to our knowledge the first case report proving the high efficacy and safety of using yet another stent retriever, namely a pRESET stent retriever in removing a fully dislocated coil in the abdominal vessels, namely in this case the CHA.