bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sense-making of autonomy and control: Comparing school leaders in public and independent schools in a Swedish case
Authors: Nordholm Daniel , Arnqvist Anders , Nihlfors Elisabet 
Published: June 2021

AbstractThis article analyses how school leaders depict their autonomy and how they make sense of the relationship between autonomy and control. Attention is drawn to three leadership focuses: the pedagogical direction of the school, decision-making over the internal organisation, and school improvement work. The article integrates survey data on Swedish school leaders (n = 1286). In addition, two theoretical categories were applied in the qualitative data analysis to explore how school leaders and local education authorities and its independent counterpart make sense of the relationship between autonomy and control: technical sense-making (TSM) and critical reflective learning. The findings showed that school leaders, both in public and independent schools, experienced a rather high degree of autonomy within the three focuses. The findings also indicated that school leaders in independent schools experienced a higher degree of autonomy regarding the pedagogical direction of the school and school improvement work. However, the effect sizes were low, indicating that the results must be considered with caution. The qualitative data analysis, in turn, revealed an overall emphasis on TSM among school leaders both in public and independent schools, reducing the possibilities for a novel and vibrant leadership. Based on these results, the article concludes that autonomy could constitute an important prerequisite for school leadership, but making sense of the relationship between autonomy and control stands out as even more important for successful school improvement.
Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds
Authors: Bilge Selva , Ergene Emre , Talak Ebru , Gokyer Seyda , Donar Yusuf Osman , Sınağ Ali , Yilgor Huri Pinar 
Published: June 2021

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.
Joint optimization of an autoencoder for clustering and embedding
Authors: Boubekki Ahcène , Kampffmeyer Michael , Brefeld Ulf , Jenssen Robert 
Published: June 2021

AbstractDeep embedded clustering has become a dominating approach to unsupervised categorization of objects with deep neural networks. The optimization of the most popular methods alternates between the training of a deep autoencoder and ak-means clustering of the autoencoder’s embedding. The diachronic setting, however, prevents the former to benefit from valuable information acquired by the latter. In this paper, we present an alternative where the autoencoder and the clustering are learned simultaneously. This is achieved by providing novel theoretical insight, where we show that the objective function of a certain class of Gaussian mixture models (GMM’s) can naturally be rephrased as the loss function of a one-hidden layer autoencoder thus inheriting the built-in clustering capabilities of the GMM. That simple neural network, referred to as the clustering module, can be integrated into a deep autoencoder resulting in a deep clustering model able to jointly learn a clustering and an embedding. Experiments confirm the equivalence between the clustering module and Gaussian mixture models. Further evaluations affirm the empirical relevance of our deep architecture as it outperforms related baselines on several data sets.
Japan’s voluntary lockdown: further evidence based on age-specific mobile location data
Authors: Watanabe Tsutomu , Yabu Tomoyoshi 
Published: June 2021

AbstractChanges in people’s behavior during the COVID-19 pandemic can be regarded as the result of two types of effects: the “intervention effect” (changes resulting from government orders for people to change their behavior) and the “information effect” (voluntary changes in people’s behavior based on information about the pandemic). Using age-specific mobile location data, we examine how the intervention and information effects differ across age groups. Our main findings are as follows. First, the age profile of the intervention effect shows that the degree to which people refrained from going out was smaller for older age groups, who are at a higher risk of serious illness and death, than for younger age groups. Second, the age profile of the information effect shows that the degree to which people stayed at home tended to increase with age for weekends and holidays. Thus, while Acemoglu et al. (2020) proposed targeted lockdowns requiring stricter lockdown policies for the oldest group in order to protect those at a high risk of serious illness and death, our findings suggest that Japan’s government intervention had a very different effect in that it primarily reduced outings by the young, and what led to the quarantining of older groups at higher risk instead was people’s voluntary response to information about the pandemic. Third, the information effect has been on a downward trend since the summer of 2020. It is relatively more pronounced among the young, so that the age profile of the information effect remains upward sloping.
‘Against Interpretation’: Petronius and art Criticism
Authors: Slater Niall W. 
Published: June 2021

For forty years a debate has raged in Petronian studies between the moralists and, for want of a better term, the anti-moralists. From Highet in the 1940's to Bacon and Arrowsmith in the 1950's and 60's, the moralists held a certain advantage. Whatever important divergences there were among these critics, all agreed on a Petronius who stood in some critical relation to his society. The dissenting voices have grown much louder of late. Ironically, the literary brilliance of Arrowsmith's New Critical reading of the Satyricon helped to turn the tide against the moralist viewpoint. The more apparent the literary sophistication of the Satyricon has become, the less willing late twentieth century readers have been to see a programmatic moral critique as its main purpose. Sullivan's view of Petronius as a ‘literary opportunist’ has come to dominate the field.With Graham Anderson's book, Eros Sophistes: Ancient Novelists at Play, the retreat from the position of Highet is now complete. We have finally reached the logical, New Critical conclusion that the Satyricon is an entirely self-contained literary game without any message whatsoever; in effect we are told that, like any serious piece of literature, the Satyricon ‘should not mean, but be’. Anderson is eager to disavow ‘the unproven conviction that every work must have a message, however diffusely or perversely expressed’.
Transcatheter closure of post-operative type 1 Gerbode defect by Amplatzer Duct Occluder 2
Authors: Kavurt Ahmet Vedat , Ece İbrahim , Bağrul Denizhan 
Published: June 2021

AbstractAcquired and congenital left ventricular to right atrial communication is rare, but nowadays, the frequency of the iatrogenic subgroup is increasing. Successful transcatheter closure of these defects with different devices has been reported. Herein, we presented successful closure of left ventricular to right atrial communication with Amplatzer Duct Occluder 2 after attempting to close with a failed Amplatzer Vascular Plug II device in a 7-year-old girl. This report supports that transcatheter closure of iatrogenic Gerbode defect with Amplatzer Duct Occluder 2 device is safe and effective.
Changes in the germinability of seeds of dicotyledonous herbs from anthropogenic and wild habitats during two initial years in a seedbank
Authors: Martinková Zdenka , Honěk Alois , Brabec Marek 
Published: June 2021

AbstractThe germinability of buried seeds changes with time, and the direction and periodicity of these changes differ among plant species. In 116 abundant dicotyledonous herb species, we investigated the changes in seed germinability that occurred during the 2-yr period following burial in the soil. We aimed to establish differences between seeds collected in “anthropogenic” (ruderal, arable land) and “wild” (grassland, forest) habitats. The seeds were buried in a field 1 mo after collection, exhumed at regular intervals, and germinated at 25 C. During the 2-yr study period, four categories of species-specific patterns of germinability changes were found: seeds demonstrating seasonal dormancy/nondormancy cycles (31 species); seeds germinating only in the first season after burial (16 species); seeds germinating steadily (38 species); and seeds whose germinability changed gradually, with increasing (7 species) or decreasing (18 species) germinability. The seeds of 6 species did not germinate at all. We found no significant difference in the frequency of these categories between species typical for anthropogenic and wild habitats. The cause for this result may be dramatic human influences (changes of agricultural practices), the pressure of which impedes the development of floras specific for certain habitats, as distinguished by the frequency of species with particular patterns of seed germinability. These frequencies varied among taxa with the growth form, seed mass, and flowering phenology of species.
Evolution of (Bio‐)Geochemical Processes and Diagenetic Alteration of Sediments Along the Tectonic Migration of Ocean Floor in the Shikoku Basin off Japan
Authors: Köster Male , Kars Myriam , Schubotz Florence , Tsang Man‐Yin , Maisch Markus , Kappler Andreas , Morono Yuki , Inagaki Fumio , Heuer Verena B. , Kasten Sabine , Henkel Susann 
Published: June 2021

AbstractBiogeochemical processes in subseafloor sediments are closely coupled to global element cycles. To improve the understanding of changes in biogeochemical conditions on geological timescales, we investigate sediment cores from a 1,180 m deep hole in the Nankai Trough offshore Japan (Site C0023) drilled during International Ocean Discovery Program Expedition 370. During its tectonic migration from the Shikoku Basin to the Nankai Trough over the past 15 Ma, Site C0023 has experienced significant changes in depositional, thermal, and geochemical conditions. By combining pore‐water, solid‐phase, and rock magnetic data, we demonstrate that a transition from organic carbon‐starved conditions with predominantly aerobic respiration to an elevated carbon burial environment with increased sedimentation occurred at ∼2.5 Ma. Higher rates of organic carbon burial in consequence of increased nutrient supply and productivity likely stimulated the onset of anaerobic electron‐accepting processes during organic carbon degradation. A significant temperature increase by ∼50°C across the sediment column associated with trench‐style sedimentation since ∼0.5 Ma could increase the bioavailability of organic matter and enhance biogenic methanogenesis. The resulting shifts in reaction fronts led to diagenetic transformation of iron (oxyhydr)oxides into pyrite in the organic carbon‐starved sediments several millions of years after burial. We also show that high amounts of reducible iron(III) which can serve as electron acceptor for microbial iron(III) reduction are preserved and still available as phyllosilicate‐bound iron. This is the first study that shows the evolution of long‐term variations of (bio‐)geochemical processes along tectonic migration of ocean floor, thereby altering the primary sediment composition long after deposition.
Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels
Authors: Fütterer Agnes , Talavera-Gutiérrez Amaia , Pons Tirso , de Celis Jesús , Gutiérrez Julio , Domínguez Plaza Verónica , Martínez-A Carlos 
Published: June 2021

AbstractEmbryonic stem cell (ESC) differentiation and somatic cell reprogramming are biological processes governed by antagonistic expression or repression of a largely common set of genes. Accurate regulation of gene expression is thus essential for both processes, and alterations in RNA processing are predicted to negatively affect both. We show that truncation of the DIDO gene alters RNA splicing and transcription termination in ESC and mouse embryo fibroblasts (MEF), which affects genes involved in both differentiation and reprogramming. We combined transcriptomic, protein interaction, and cellular studies to identify the underlying molecular mechanism. We found that DIDO3 interacts with the helicase DHX9, which is involved in R-loop processing and transcription termination, and that DIDO3-exon16 deletion increases nuclear R-loop content and causes DNA replication stress. Overall, these defects result in failure of ESC to differentiate and of MEF to be reprogrammed. MEF immortalization restored impaired reprogramming capacity. We conclude that DIDO3 has essential functions in ESC differentiation and somatic cell reprogramming by supporting accurate RNA metabolism, with its exon16-encoded domain playing the main role.
Couples’ experiences with expanded carrier screening: evaluation of a university hospital screening offer
Authors: van Dijke Ivy , Lakeman Phillis , Sabiri Naoual , Rusticus Hanna , Ottenheim Cecile P. E. , Mathijssen Inge B. , Cornel Martina C. , Henneman Lidewij 
Published: June 2021

AbstractPreconception carrier screening offers couples the possibility to receive information about the risk of having a child with a recessive disorder. Since 2016, an expanded carrier screening (ECS) test for 50 severe autosomal recessive disorders has been available at Amsterdam Medical Center, a Dutch university hospital. This mixed-methods study evaluated the experiences of couples that participated in the carrier screening offer, including high-risk participants, as well as participants with a general population risk. All participants received genetic counselling, and pre- (n = 132) and post-test (n = 86) questionnaires and semi-structured interviews (n = 16) were administered. The most important reason to have ECS was to spare a future child a life with a severe disorder (47%). The majority of survey respondents made an informed decision (86%), as assessed by the Multidimensional Measure of Informed Choice. Among the 86 respondents, 27 individual carriers and no new carrier couples were identified. Turn-around time of the test results was considered too long and costs were perceived as too high. Overall, mean levels of anxiety were not clinically elevated. High-risk respondents (n = 89) and pregnant respondents (n = 13) experienced higher levels of anxiety before testing, which decreased after receiving the test result. Although not clinically significant, distress was on average higher for carriers compared to non-carriers (p < 0.0001). All respondents would opt for the test again, and 80.2% would recommend it to others. The results suggest that ECS should ideally be offered before pregnancy, to minimise anxiety. This study could inform current and future implementation initiatives of preconception ECS.
Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
Authors: Hatzopoulos Georgios N. , Kükenshöner Tim , Banterle Niccolò , Favez Tatiana , Flückiger Isabelle , Hamel Virginie , Andany Santiago , Fantner Georg E. , Hantschel Oliver , Gönczy Pierre 
Published: June 2021

AbstractCentrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. The importance of this architecture has been difficult to decipher notably because of the lack of precise tools to modulate the underlying assembly reaction. Here, we developed monobodies against Chlamydomonas reinhardtii SAS-6, characterizing three in detail with X-ray crystallography, atomic force microscopy and cryo-electron microscopy. This revealed distinct monobody-target interaction modes, as well as specific consequences on ring assembly and stacking. Of particular interest, monobody MBCRS6-15 induces a conformational change in CrSAS-6, resulting in the formation of a helix instead of a ring. Furthermore, we show that this alteration impairs centriole biogenesis in human cells. Overall, our findings identify monobodies as powerful molecular levers to alter the architecture of multi-protein complexes and tune centriole assembly.
Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum
Authors: Evers Felix , Cabrera-Orefice Alfredo , Elurbe Dei M. , Kea-te Lindert Mariska , Boltryk Sylwia D. , Voss Till S. , Huynen Martijn A. , Brandt Ulrich , Kooij Taco W. A. 
Published: June 2021

AbstractOur current understanding of mitochondrial functioning is largely restricted to traditional model organisms, which only represent a fraction of eukaryotic diversity. The unusual mitochondrion of malaria parasites is a validated drug target but remains poorly understood. Here, we apply complexome profiling to map the inventory of protein complexes across the pathogenic asexual blood stages and the transmissible gametocyte stages of Plasmodium falciparum. We identify remarkably divergent composition and clade-specific additions of all respiratory chain complexes. Furthermore, we show that respiratory chain complex components and linked metabolic pathways are up to 40-fold more prevalent in gametocytes, while glycolytic enzymes are substantially reduced. Underlining this functional switch, we find that cristae are exclusively present in gametocytes. Leveraging these divergent properties and stage dynamics for drug development presents an attractive opportunity to discover novel classes of antimalarials and increase our repertoire of gametocytocidal drugs.
Global coastal attenuation of wind-waves observed with radar altimetry
Authors: Passaro Marcello , Hemer Mark A. , Quartly Graham D. , Schwatke Christian , Dettmering Denise , Seitz Florian 
Published: June 2021

AbstractCoastal studies of wave climate and evaluations of wave energy resources are mainly regional and based on the use of computationally very expensive models or a network of in-situ data. Considering the significant wave height, satellite radar altimetry provides an established global and relatively long-term source, whose coastal data are nevertheless typically flagged as unreliable within 30 km of the coast. This study exploits the reprocessing of the radar altimetry signals with a dedicated fitting algorithm to retrieve several years of significant wave height records in the coastal zone. We show significant variations in annual cycle amplitudes and mean state in the last 30 km from the coastline compared to offshore, in areas that were up to now not observable with standard radar altimetry. Consequently, a decrease in the average wave energy flux is observed. Globally, we found that the mean significant wave height at 3 km off the coast is on average 22% smaller than offshore, the amplitude of the annual cycle is reduced on average by 14% and the mean energy flux loses 38% of its offshore value.
Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide
Authors: Santucci Pierre , Greenwood Daniel J. , Fearns Antony , Chen Kai , Jiang Haibo , Gutierrez Maximiliano G. 
Published: June 2021

AbstractTo be effective, chemotherapy against tuberculosis (TB) must kill the intracellular population of the pathogen, Mycobacterium tuberculosis. However, how host cell microenvironments affect antibiotic accumulation and efficacy remains unclear. Here, we use correlative light, electron, and ion microscopy to investigate how various microenvironments within human macrophages affect the activity of pyrazinamide (PZA), a key antibiotic against TB. We show that PZA accumulates heterogeneously among individual bacteria in multiple host cell environments. Crucially, PZA accumulation and efficacy is maximal within acidified phagosomes. Bedaquiline, another antibiotic commonly used in combined TB therapy, enhances PZA accumulation via a host cell-mediated mechanism. Thus, intracellular localisation and specific microenvironments affect PZA accumulation and efficacy. Our results may explain the potent in vivo efficacy of PZA, compared to its modest in vitro activity, and its critical contribution to TB combination chemotherapy.
A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab
Authors: Nakamura Hitomi , Yoshikawa Moeka , Oda-Ueda Naoko , Ueda Tadashi , Ohkuri Takatoshi 
Published: June 2021

AbstractGenerally, intermolecular disulfide bond contribute to the conformational protein stability. To identify sites where intermolecular disulfide bond can be introduced into the Fab’s constant domain of the therapeutic IgG, Fab mutants were predicted using the MOE software, a molecular simulator, and expressed in Pichia pastoris. SDS-PAGE analysis of the prepared Fab mutants from P. pastoris indicated that among the nine analyzed Fab mutants, the F130C(H):Q124C(L), F174C(H):S176C(L), V177C(H):Q160C(L), F174C(H):S162C(L), F130C(H):S121C(L), and A145C(H):F116C(L) mutants mostly formed intermolecular disulfide bond. All these mutants showed increased thermal stability compared to that of Fab without intermolecular disulfide bond. In the other mutants, the intermolecular disulfide bond could not be completely formed, and the L132C(H):F118C(L) mutant showed only a slight decrease in binding activity and β-helix content, owing to the exertion of adverse intermolecular disulfide bond effects. Thus, our comprehensive analysis reveals that the introduction of intermolecular disulfide bond in the Fab’s constant domain is possible at various locations. These findings provide important insights for accomplishing human Fab stabilization.