bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptable Automated Interpretation of Rapid Diagnostic Tests Using Few-Shot Learning
Authors: Arumugam Siddarth , Ma Jiawei , Macar Uzay , Han Guangxing , McAulay Kathrine , Ingram Darrell , Ying Alex , Colburn David A. M. , Stanciu Robert , Grys Thomas , Chang Shih-Fu , Sia Samuel K. 
Published: June 2021

AbstractPoint-of-care lateral-flow assays (LFAs) are becomingly increasingly prevalent for diagnosing individual patient disease status and surveying population disease prevalence in a timely, scalable, and cost-effective manner, but a central challenge is to assure correct assay operation and results interpretation as the assays are manually performed in decentralized settings. A smartphone-based software can automate interpretation of an LFA kit, but such algorithms typically require a very large number of images of assays tested with validated specimens, which is challenging to collect for different assay kits, especially for those released during a pandemic. Here, we present an approach – AutoAdapt LFA – that uses few-shot learning, an approach used in other applications such as computer vision and robotics, for accurate and automated interpretation of LFA kits that requires a small number of validated images for training. The approach consists of three components: extraction of membrane and zone areas from an image of the LFA kit, a self-supervised encoder that employs a feature extractor trained with edge-filtered patterns, and few-shot adaptation that enables generalization to new kits using limited validated images. From a base model pre-trained on a commercial LFA kit, we demonstrated the ability of adapted models to interpret results from five new COVID-19 LFA kits (three detecting antigens for diagnosing active infection, and two detecting antibodies for diagnosing past infection). Specifically, using just 10 to 20 images of each new kit, we achieved accuracies of 99% to 100% for each kit. The server-hosted algorithm has an execution time of approximately 4 seconds, which can potentially enable quality assurance and linkage to care for users operating new LFAs in decentralized settings.
The PLETHORA/PIN-FORMED/AUXIN network mediates terminal prehaustorium formation in the parasitic plant Striga hermonthica
Authors: Xiao Ting Ting , Kirschner Gwendolyn K. , Kountche Boubacar A. , Jamil Muhammad , Maria Savina , Lube Vinicius , Mironova Victoria , al Babili Salim , Blilou Ikram 
Published: June 2021

SummaryThe parasitic plant Striga hermonthica invades the host root through the formation of a haustorium and has detrimental impacts on cereal crops. The haustorium is derived directly from the differentiation of the Striga radicle. Currently, how Striga root cell lineages are patterned and the molecular mechanisms leading to radicle differentiation shortly after germination remain unclear. In this study, we determined the developmental-morphodynamic programs that regulate terminal haustorium formation in S. hermonthica at spatiotemporal and cellular resolutions. We showed that in S. hermonthica roots, meristematic cells first undergo multiplanar divisions, which decrease during growth and correlate with reduced expression of the stem cell regulator PLETHORA1. We also found that PIN-FORMED (PIN) proteins undergo a shift in polarity. Using the layout of the root structure and the polarity of outer-membrane PIN proteins, we constructed a mathematical model of auxin transport that explains the auxin distribution patterns observed during S. hermonthica root growth. Our results reveal a fundamental molecular and cellular framework governing the switch of S. hermonthica roots from the vegetative to the invasive state by inducing meristem differentiation through auxin excretion to the environment and explain how asymmetric PIN polarity controls auxin distribution to maintain meristem activity and sustain root growth.
Dynamically expressed ELAV is required for learning and memory in bees
Authors: Ustaoglu Pinar , Gill Jatinder Kaur , Doubovetzky Nicolas , Haussmann Irmgard U. , Devaud Jean-Marc , Soller Matthias 
Published: June 2021

AbstractChanges in gene expression are a hallmark of learning and memory consolidation. Little is known about how alternative mRNA processing, particularly abundant in neuron-specific genes, contributes to these processes. Prototype RNA binding proteins of the neuronally expressed ELAV/Hu family are candidates for roles in learning and memory, but their capacity to cross-regulate and take over each other’s functions complicate substantiation of such links. Therefore, we focused on honey bees, which have only a single elav family gene. We find that honey bee elav contains a microexon, which is evolutionary conserved between invertebrates and humans. RNAi knockdown of elav demonstrates that ELAV is required for learning and memory in bees. Indicative of a role as immediate early gene, ELAV is dynamically expressed with altered alternative splicing and subcellular localization in mushroom bodies, but not in other brain parts. Expression and alternative splicing of elav change during memory consolidation illustrating an alternative mRNA processing program as part of a local gene expression response underlying memory formation. Although the honey bee genome encodes only a single elav gene, functional diversification is achieved by alternative splicing.
Yeast cells under glucose-limitation environment need increased response cost for osmostress defense
Authors: Shen Wenting , Gao Ziqing , Chen Kaiyue , Ouyang Qi , Luo Chunxiong 
Published: June 2021

AbstractCells always make responses to environmental changes, involving dynamic expression of tens to hundreds of proteins. This response system may demand substantial cost and thus affect cell growth. Here, we studied the cell’s responses to osmostress under glucose-limitation environments. Through analyzed thirteen osmotic-downstream proteins and two related transcription factors, we found that the cells required stronger responses under low glucose concentrations than normal glucose condition after being stimulated by osmostress, even the cell growth rate was unchanged in these two constant conditions. We proposed and verified that under a glucose-limitation environment, the glycolysis intermediates were limited (defense reserve saving), which caused that cells needed more glycerol production enzymes to adapt to the osmostress. Further experiments proved that this ‘defense reserve-saving’ strategy required cells to spend more response cost when facing stress, which on the other hand, enhanced the fitness for the coming environment variations via protein accumulation reserve.
Multivariate genome-wide association study on tissue-sensitive diffusion metrics identifies key molecular pathways for axonal growth, synaptogenesis, and astrocyte-mediated neuroinflammation
Authors: Fan Chun Chieh , Loughnan Robert , Makowski Carolina , Pecheva Diliana , Chen Chi-Hua , Hagler Donald J. , Thompson Wesley K. , Parker Nadine , van der Meer Dennis , Frei Oleksandr , Andreassen Ole A. , Dale Anders M. 
Published: June 2021

AbstractThe molecular determinants of tissue composition of the human brain remain largely unknown. Recent genome-wide association studies (GWAS) on this topic have had limited success due to methodological constraints. Here, we apply advanced whole-brain analyses on multi-shell diffusion imaging data and multivariate GWAS to two large scale imaging genetic datasets (UK Biobank and the Adolescent Brain Cognitive Development study) to identify and validate genetic association signals. We discovered 503 unique genetic loci that have impact on multiple regions of human brain. Among them, more than 79% were validated in either of two large-scale independent imaging datasets. Key molecular pathways involved in axonal growth, astrocyte-mediated neuroinflammation, and synaptogenesis during development were found to significantly impact the measured variations in tissue-specific imaging features. Our results shed new light on the biological determinants of brain tissue composition and their potential overlap with the genetic basis of neuropsychiatric disorders.
Protein Kinase Cγ Mutations Drive Spinocerebellar Ataxia Type 14 by Impairing Autoinhibition
Authors: Pilo Caila A. , Baffi Timothy R. , Kornev Alexandr P. , Kunkel Maya T. , Malfavon Mario , Chen Dong-Hui , Huang Liang-Chin , Longman Cheryl , Kannan Natarajan , Raskind Wendy , Gonzalez David J. , Taylor Susan S. , Gorrie George , Newton Alexandra C. 
Published: June 2021

AbstractSpinocerebellar ataxia type 14 (SCA14) is a neurodegenerative disease caused by germline variants in the diacylglycerol (DG)/Ca2+-regulated protein kinase C gamma (PKCγ), leading to Purkinje cell degeneration and progressive cerebellar dysfunction. The majority of the approximately 50 identified variants cluster to the DG-sensing C1 domains. Here, we use a FRET- based activity reporter to show that ataxia-associated PKCγ mutations enhance basal activity by compromising autoinhibition. Although impaired autoinhibition generally leads to PKC degradation, the C1 domain mutations protect PKCγ from phorbol ester-induced downregulation. Furthermore, it is the degree of disrupted autoinhibition, not changes in the amplitude of agonist- stimulated activity, that correlate with disease severity. This enhanced basal signaling rewires the brain phosphoproteome, as assessed by phosphoproteomic analysis of cerebella from mice expressing a human PKCγ transgene harboring a SCA14 C1 domain mutation, H101Y. Validating that the pathology arises from disrupted autoinhibition, we show that the degree of impaired autoinhibition correlates inversely with age of disease onset in patients: mutations that cause high basal activity are associated with early onset, whereas those that only modestly increase basal activity, including a previously undescribed variant, D115Y, are associated with later onset. Molecular modeling indicates that almost all SCA14 variants that are not in the C1 domains are at interfaces with the C1B domain, and bioinformatics analysis reveals that variants in the C1B domain are under-represented in cancer. Thus, clustering of SCA14 variants to the C1B domain provides a unique mechanism to enhance PKCγ basal activity while protecting the enzyme from downregulation, deregulating the cerebellar phosphoproteome.One Sentence SummarySCA14 driver mutations in PKCγ impair autoinhibition, with defect correlating inversely with age of disease onset.
Analysis of rhizosphere bacterial diversity of Angelica dahurica var. formosana based on different experimental sites and varieties (strains)
Authors: Jiang Meiyan , Yao Fei , Yang Yunshu , Zhou Yang , Hou Kai , Chen Yinyin , Feng Dongju , Wu Wei 
Published: June 2021

AbstractOnly with good producing areas and good germplasm can good medicinal materials be produced. In this study, in order to explore the effects of soil environment and germplasm on yield and quality of Angelica dahurica var. formosana from microorganism level, Illumina MiSeq was used to study the rhizosphere bacterial diversity of A. dahurica var. formosana based on different origin and varieties (strains). The results showed that the bacterial community of A. dahurica var. formosana was stable and conserved to a certain extent, and the bacteria of Proteobacteria and Firmicutes might play an important role in improving the yield and quality of A. dahurica var. formosana. Soil variables were the key factors affecting the rhizosphere bacterial community composition of A. dahurica var. formosana. These results are of great significance for further understanding the growth promotion mechanism of beneficial rhizosphere bacteria, reducing fertilizer application, expanding planting area, improving soil environment, and improving yield and quality.ImportanceRhizosphere microorganisms play an important role in plant growth and accumulation of secondary metabolites. In this study, it was found for the first time that the rhizosphere bacterial community of A. dahurica var. formosana was relatively stable and conserved which could adapt to different environmental changes and resist the interference of external factors to a certain extent, providing a theoretical basis for distant planting and promotion of planting. The bacterial taxa of Proteobacteria and Firmicutes might play an important role in improving the yield and quality of A. dahurica var. formosana. This provided a theoretical basis for the exploitation and utilization of rhizosphere microbial resources of A. dahurica var. formosana to artificially control the soil environment and provided a new idea for increasing yield and quality of A. dahurica var. formosana.
Impulsivity traits and the rational cognitive process of entrepreneurship: empirical evidence from Vietnam
Authors: Doan Xuan Hau , Le Trung Thanh , Duong Cong Doanh , Nguyen Thi Phuong Linh , Tran Duc Dung , Tran Thi Phuong Hien 
Published: June 2021

PurposeThis study aims to integrate predictions from clinical psychology and UPPS impulsivity with the theory of planned behaviors (TPB) to draw a conceptual framework and test the prediction that attention deficit hyperactivity disorder (ADHD) symptoms, impulsivity would contribute to the prediction of the reasoned cognitive process of entrepreneurship over and above key predictors from an extended TPB model.Design/methodology/approachThis study utilized a sample of 2,482 students from 14 universities/institutes in Vietnam; confirmatory factor analysis was employed to test the validity and reliability. Then, regression analysis with PROCESS macro approach (5,000 bootstrap sample and 95% confidence interval) was employed to estimate the association paths and multiple mediators.FindingsThe study reveals that ADHD symptoms and impulsivity substantially contribute to the exploration of an entrepreneurial intention throughout TPB predictors, with those higher in ADHD symptoms and impulsivity having higher intentions to engage in business venturing. Moreover, UPPS impulsiveness might valuably be incorporated with TPB predictors while predicting behaviors that are often examined as the process of rational cognitive strategies business venturing.Practical implicationsThis study showed that a start-up business can be seen as a career choice for students who exhibit extensive ADHD symptoms to use their talents effectively, thus contributing to creating value for society and improving personal well-being.Originality/valueThis article stood to make contributions to entrepreneurship literature by investigating the effects of ADHD symptoms, four impulsivity traits on an entrepreneurial intention via three precursors in TPB, including attitude toward entrepreneurship, subjective norms and perceived behavioral control.
A scale for measuring organizational risk management maturity in the supply chain
Authors: Dellana Scott , Rowe William J. , Liao Ying 
Published: June 2021

PurposeThe purpose of this research is to develop a validated general measure of supply chain risk management maturity (SCRMM) to assess organizational risk management maturity in the context of the supply chain (SC).Design/methodology/approachDimensions and statements measuring SCRMM were developed through a literature review and consultation with experts. The instrument was refined through a pilot study and a full-scale study of 140 SC managers in the USA. A final SCRMM instrument, consisting of 25 statements, was obtained through scale purification, exploratory factor analysis, reliability analysis, and confirmatory factor analysis of construct validity. Cluster analysis was conducted to characterize the organizational groupings with respect to the instrument dimensions.FindingsSCRMM was found to be comprised of the three main dimensions of SC Risk Management Orientation, Enterprise Risk Management Integration (ERMI), and SC Risk Collaboration. ERMI was found to be comprised of the three sub-dimensions of SC Risk Mitigation, Improvement of Risk Management Processes, and Organization Internal Risk Management. Cluster analysis revealed three groups characterized according to SCRMM as leaders, followers, and laggards.Research limitations/implicationsThe findings are based on a sample in the USA, so the SCRMM scale may not generalize to supply chains in other countries or geographic regions.Practical implicationsThe instrument provides a self-assessment and benchmark tool for businesses to advance their SC risk management through different stages of maturity.Originality/valueThis is a pioneering general instrument that treats risk management maturity of the organization in the context of the SC. Participants span many industries and SC positions.
A model for work packages optimization in results-based-finance projects
Authors: Shalaby Amira , Ezeldin A. Samer 
Published: June 2021

PurposeIn many developing countries, the sanitation sector constitutes a major part of their strategic plans of reform. Yet with the very limited budget of the public treasury, countries opt to major lending institutions for funds. “Results-Based-Finance” is a new funding mechanism that has proven its efficiency in achieving the necessary reform in sanitation sectors. Due to the complexity of the funding tool, it is crucial to be able to decompose the project into smaller packages to be able to effectively control the project. The objective of this paper is to reach an optimum packaging scheme that enables the project to be successfully managed through better planning and cost control practices.Design/methodology/approachWith the aid of Unified Modelling Language (UML), an algorithm is developed to map the logic behind the model suggested with detailed illustrations of its different modules. Object-oriented processes and operations are modeled using different diagrams of the language, which automatically generate the optimum packaging combination. The packaging model is then implemented via a number of computer-aided programs. The Microsoft Excel 2019 is used for calculation purposes. Visual Basic for Applications (VBA) programming language is used to make the model user-friendly for non-engineering stakeholders. The Palisade's Decision Tools Suite is used for the optimization processFindingsThe model is validated through a case study of a mega sanitation project located in Egypt. The model output is not only the content of the packages but also a complete managing plan which demonstrates many useful information to the decision-makers and government officials.Originality/valueThe research aim is to provide the construction industry with a tool that makes the packaging process of mega projects funded through the “Results-Based-Finance” mechanism, done in an automated manner. Moreover, the packages are selected in a way to optimize the project cashflow. Having the optimum package size shall ensure better planning and a more accurate cost control. Yet it is a challenging task; especially, when the project cash flow is very sensitive and intolerant to delays like in the “Results-Based-Finance” mechanism.
How social capital affects willingness of farmers to accept low-carbon agricultural technology (LAT)? A case study of Jiangsu, China
Authors: Liu Chunhui , Zheng Huawei 
Published: June 2021

PurposeLow-carbon agricultural technology (LAT) extension is a key strategy for the agricultural sector to address climate change. Social capital, which consists of social networks, trust and norms, can play an active LAT extension role. This paper aims to analyze the mechanism of the role of social capital in the process of LAT extension.Design/methodology/approachQuestionnaire data from six counties in Jiangsu, China, were used to measure social capital and analyze its effect on LAT extension using logistic regression. Data from 27 interviews were used to analyze the LAT extension experiences and problems.FindingsLAT is mainly deployed by the government to farmers and distributed among them. In this process, the village officials who form parts of the government’s composition and the villagers play a dual role that facilitates a close link between them and the farmers and ensures LAT integration. However, social norms did not play a significant role in the process.Practical implicationsFarmers’ acceptance of LAT is based solely on the trade-off between local networks’ benefits and trust in local villagers and village officials. LAT-related laws and technical measures, thus are essential to strengthen LAT practices’ authority and incorporate LAT-based agricultural production as the norm of production behavior.Originality/valueThis paper provides an insight into the process and essence of farmers’ acceptance of LAT, which provides theoretical lessons for the LAT extension in China and indeed other developing countries.
Empowering woman through legal reforms-evidence from Saudi Arabian context
Authors: Rizvi Lubna Javed , Hussain Zahid 
Published: June 2021

Purpose
This current paper aims to review the literature for two main reasons. First, to outline the propositions of women empowerment in manifesto of “Vision 2030”and how these implementations have added to woman’s success in different facets of the economy. Second, to review the current reforms made to empower woman under Saudi law published in 08/2019 when the Council of Ministers presented numerous revisions to civil and labour laws that increase women’s rights and discourse concerns of travel, employment and finance.

Design/methodology/approach
The investigation is found on both an analysis of the secondary literature including records and information pertaining to the current economic alterations and improvements influencing women.

Findings
Despite the various limitations in Saudi Arabia, many Saudi women are joining the workforce. Nowadays, Saudi female are selecting new professions which were previously unexplored. Study revealed that Saudi females in different organizational framework are equally competent as male in higher ranks, if not slightly higher (Al-Shamrani, 2015). Within this context, Saudi Arabia was labelled as one of the economies with the most progress towards gender equality since 2017 in the World Bank’s Women, Business and The Law 2020 report; the report attributed this advancement to the increases in freedom of movement and economic opportunities for women as a result of the aforementioned reforms.

Originality/value
This paper has reflected on the success of measures by government to empower woman in the thirst to fulfill “Vision 2030” in Saudi Arabian context.