bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of GLPG1205, a GPR84 Modulator, on CYP2C9, CYP2C19, and CYP1A2 Enzymes: In Vitro and Phase 1 Studies
Authors: Desrivot Julie , Van Kaem Tim , Allamassey Lisa , Helmer Eric 
Published: May 2021

AbstractGLPG1205 is a novel agent being investigated for the treatment of idiopathic pulmonary fibrosis. GLPG1205 may be concomitantly administered with pirfenidone in future clinical development; therefore, the potential for GLPG1205 to interact with enzymes involved in the metabolism of pirfenidone (cytochrome P450 [CYP] 1A2, CYP2C9, 2C19) was evaluated. In vitro experiments indicated weak inhibition of CYP1A2 and moderate but reversible inhibition of CYP2C9 and CYP2C19 by GLPG1205. A phase 1 randomized, double‐blind crossover study in 14 healthy males (NCT02623296) evaluated the effect of GLPG1205 100 mg or placebo (once daily for 12 days) on the single‐dose pharmacokinetics of a cocktail of CYP1A2, CYP2C9, and CYP2C19 substrates (coadministered on day 13). GLPG1205 had no effect on the exposure of CYP2C9 and CYP1A2 substrates or metabolites; however, a trend toward increased omeprazole (CYP2C19 substrate) exposure was observed. Although considered not clinically relevant, GLPG1205 increased the elimination rate of 5‐hydroxyomeprazole (CYP2C19 metabolite) 1.16‐fold versus placebo. GLPG1205 had no effect on the elimination of all other substrates or metabolites. GLPG1205 had a favorable safety and tolerability profile. In conclusion, GLPG1205 100 mg once daily does not interact with CYP2C9, CYP2C19, or CYP1A2 to a clinically relevant extent and may be administered concomitantly with drugs metabolized by these enzymes.
DNA‐Compatible Click Reaction Employing In Situ Generated Azides from Boronic Acids
Authors: An Yulong , Yan Hao , Dong Zhenzhen , Satz Alexander L. 
Published: May 2021

AbstractAn efficient method for the synthesis of DNA‐conjugated 1,2,3‐triazoles is copper (II) [Cu(II)‐β‐cyclodextrin]‐mediated Huisgen cycloaddition (“click reaction”) of DNA‐conjugated alkynes with azides. However, a diverse array of building blocks is required to produce useful DNA encoded libraries, and the commercial availability of azides is limited. The method described herein generates azides in situ from aryl borates and TMSN3, which then further react with DNA‐conjugated terminal alkynes. © 2021 Wiley Periodicals LLC.Basic Protocol 1: Conjugation of PEG linker to DNA headpieceBasic Protocol 2: DNA conjugated terminal alkyne preparationBasic Protocol 3: DNA compatible one‐pot click reactionBasic Protocol 4: LCMS monitoring
LINC01133 promotes hepatocellular carcinoma progression by sponging miR‐199a‐5p and activating annexin A2
Authors: Yin Dan , Hu Zhi‐Qiang , Luo Chu‐Bin , Wang Xiao‐Yi , Xin Hao‐Yang , Sun Rong‐Qi , Wang Peng‐Cheng , Li Jia , Fan Jia , Zhou Zheng‐Jun , Zhou Jian , Zhou Shao‐Lai 
Published: May 2021

AbstractBackgroundLong noncoding RNAs (lncRNAs) are functionally associated with cancer development and progression. Although gene copy number variation (CNV) is common in hepatocellular carcinoma (HCC), it is not known how CNV in lncRNAs affects HCC progression and recurrence. We aimed to identify a CNV‐related lncRNA involved in HCC progression and recurrence and illustrate its underlying mechanisms and prognostic value.MethodsWe analyzed the whole genome sequencing (WGS) data of matched cancerous and noncancerous liver samples from 49 patients with HCC to identify lncRNAs with CNV. The results were validated in another cohort of 238 paired HCC and nontumor samples by TaqMan copy number assay. We preformed Kaplan‐Meier analysis and log‐rank test to identify lncRNA CNV with prognostic value. We conducted loss‐ and gain‐of‐function studies to explore the biological functions of LINC01133 in vitro and in vivo. The competing endogenous RNAs (ceRNAs) mechanism was clarified by microRNA sequencing (miR‐seq), quantitative real‐time PCR (qRT‐PCR), western blot, and dual‐luciferase reporter assays. We confirmed the binding mechanism between lncRNA and protein by RNA pull‐down, RNA immunoprecipitation, qRT‐PCR, and western blot analyses.ResultsGenomic copy numbers of LINC01133 were increased in HCC, which were positively related with the elevated expression of LINC01133. Increased copy number of LINC01133 predicted the poor prognosis in HCC patients. LINC01133 overexpression in HCC cells promoted proliferation and aggressive phenotypes in vitro, and facilitated tumor growth and lung metastasis in vivo, whereas LINC01133 knockdown had the opposite effects. LINC01133 sponged miR‐199a‐5p, resulting in enhanced expression of SNAI1, which induced epithelial‐to‐mesenchymal transition (EMT) in HCC cells. In addition, LINC01133 interacted with Annexin A2 (ANXA2) to activate the ANXA2/STAT3 signaling pathway.ConclusionsLINC01133 promotes HCC progression by sponging miR‐199a‐5p and interacting with ANXA2. LINC01133 CNV gain is predictive of poor prognosis in patients with HCC.
Yeast autonomously replicating sequence (ARS): Identification, function, and modification
Authors: Tan Xiao‐Yu , Wu Xiao‐Le , Han Ming‐Zhe , Wang Li , Xu Li , Li Bing‐Zhi , Yuan Ying‐Jin 
Published: May 2021

AbstractEukaryotic DNA replication begins with multiple origins of replication (ORIs) and thus exploring and identifying ORIs is essential for further understanding of the DNA replication process. In budding yeast, certain autonomously replicating sequences (ARSs) initiate the replication as ORIs, maintaining the stability of chromosomes and plasmids during genome replication. Currently, ARS identification was further facilitated by the DNA microarray technology and bioinformatics, which is based on the previous experimental methods. The structural and functional properties of ARSs on yeast chromosomes are gradually explored in this field. In addition to the function of initiating replication, there is a growing interest for researchers in the effects of ARSs on gene silencing and expression of genes, particularly the relationship between ARSs and chromosome structure. In this review, we summarized the identification methods of ARSs, especially for the bioinformatics prediction methods over the past few years. The functions of ARSs are discussed in this research, moreover, ARS modification that combined with the high‐throughput sequencing was elaborated as well, shedding further light on the understanding of the roles of ARSs, and providing deep insights towards the optimization of ARSs.
Performance and analysis of retail store‐centered microgrids with solar photovoltaic parking lot, cogeneration, and battery‐based hybrid systems
Authors: Shah Kunal K. , George Dane , Swan Lukas , Pearce Joshua M. 
Published: May 2021

AbstractTo examine the potential of distributed microgrids using sustainable energy sources centered on retail store parking lots, this study provides a methodology to simulate medium‐scale solar photovoltaic (PV) + combined heat and power (CHP) + battery hybrid microgrid systems deployed at big box retail stores. First, a method is provided to agglomerate 15‐min load data for a community of residences in the region of the store to provide baseline electricity load profiles. The systems are then modeled using dispatch strategies previously shown to be stable for smaller, but more dynamic loaded systems. The methodology is demonstrated with a case study for a Walmart Supercenter located in Nova Scotia, Canada. The electricity generated by each component of the hybrid system is coupled and optimized to fulfill the electric demands of the local community. The results provide the total electricity generated by the PV + CHP + battery hybrid system, total operating hours by each unit, fuel consumed, and hourly energy produced by each unit. The results show that such microgrid systems could serve the electrical needs of ˜1000 people (350 residences) for each parking lot of 3.5 MW PV system and CHP unit of 1 MW. The technical viability of this approach warrants future work.
Role ofSemisulcospira gottscheiextract as medicinal food on reflux esophagitis in rats
Authors: Nam Hyeon Hwa , Yang Sungyu , Kim Hyo Seon , Kim Min Jee , Kim Joong Sun , Lee Ji Hye 
Published: May 2021

AbstractGastroesophageal reflux disease (GERD) is a globally prevalent disease and results from a reflux of gastric contents into the esophagus. Existing synthetic drug‐based treatments for GERD have various drawbacks including refractory symptoms, relapse, or resistance due to long‐term use or may result in mucosal degeneration, polyps, and osteoporosis.Semisulcospira gottschei(SE), a freshwater snail, has been generally consumed as a food source due to its excellent flavor and nutritional value in Korea and considered to have therapeutic properties for various diseases including dyspepsia, stomachache, and hepatic diseases. The present study aims to investigate whetherSemisulcospira gottscheiextract (SGE) has a protective effect on reflux esophagitis‐induced rat models. The anti‐inflammatory effects of SGE were evaluated via NO production in LPS‐induced Raw 264.7 macrophage. And the protection effects of SGE were analyzed by assessing the amelioration of mucosal damage and expression of inflammation‐associated proteins in reflux esophagitis (RE) rats. Our results indicate that SGE significantly suppressed NO production in LPS‐induced raw 264.7 cells without any cytotoxicity. We observed mucosal lesions and histological changes in the esophagus of RE control rats. However, SGE treatment markedly ameliorated mucosal lesion ratio indicated through histological changes. SGE administration suppressed the expression of proteins related to inflammation, such as p‐NF‐κB, p‐IκBα, COX‐2, and TNF‐α, in esophageal tissue. Moreover, SGE elevated the expression of claudin‐5, which is a tight junction protein, involved in barrier function of epithelium and endothelium. The results suggest that SGE is useful as a medicinal food in esophagitis and may be helpful in developing effective treatment protocols for GERD.
Alterations of coagulation and fibrinolysis in patients with blunt splenic injury after splenic artery embolization
Authors: Ren Chuanzeng , Lu Huadong , Xia Honghai , Zhang Jia , Cao Bin , Wang Ying , Lu Dong , Cao Rongge 
Published: May 2021

AbstractBackgroundThrombotic complications following splenectomy have been documented. However, there has been sparse literature regarding thrombotic complications following splenic artery embolization (SAE).The objective of this study was to determine changes in coagulation and fibrinolysis and assess the thrombotic risk after SAE in patients with blunt splenic injury (BSI).MethodsThis study included 38 BSI patients who were hemodynamically stable on admission. SAE was performed if the splenic injury was classed as grade III or greater and had no requirement of immediate surgery. Platelet (PLT), fibrinogen (FIB), D‐dimers (D‐D), fibrinogen/fibrin degradation products (FDP), antithrombin III (AT III), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), hemoglobin (Hb), and hematocrit (Hct) were measured before SAE procedures and then 1d, 3d, and 7d after SAE.ResultsThe technical success rate of SAE and the splenic salvage rate were 100%. There was no mortality. Compared with pre‐SAE values, the levels of PLT, FIB, D‐D, and FDP increased significantly at 3 days and 7 days after SAE (p < 0.05). However, AT III, PT, APTT, TT, Hb, and Hct showed no statistically significant difference at 1d, 3d, and 7d after SAE (p > 0.05).ConclusionAlterations in PLT and hemostatic parameters might contribute to the increased risk of thrombotic complications in BSI patients undergoing SAE. Thromboembolism following SAE should be considered and thrombotic prophylaxis should be recommended.
Diverse University Students Across the United States Reveal Promising Pathways to Hunter Recruitment and Retention
Authors: Vayer Victoria R. , Larson Lincoln R. , Peterson M. Nils , Lee Kangjae Jerry , Von Furstenberg Richard , Choi Daniel Y. , Stevenson Kathryn , Ahlers Adam A. , Anhalt‐Depies Christine , Bethke Taniya , Bruskotter Jeremy , Chizinski Christopher J. , Clark Brian , Dayer Ashley A. , Ghasemi Benjamin , Gigliotti Larry , Graefe Alan , Irwin Kris , Keith Samuel J. , Kelly Matt , Kyle Gerard , Metcalf Elizabeth , Morse Wayde , Needham Mark D. , Poudyal Neelam , Quartuch Michael , Rodriguez Shari , Romulo Chelsie , Sharp Ryan L. , Siemer William , Springer Matt , Stedman Richard , Stein Taylor , Van Deelen Tim , Whiting Jason , Winkler Richelle L. , Woosnam Kyle Maurice 
Published: May 2021

ABSTRACTDeclining participation in hunting, especially among young adult hunters, affects the ability of state and federal agencies to achieve goals for wildlife management and decreases revenue for conservation. For wildlife agencies hoping to engage diverse audiences in hunter recruitment, retention, and reactivation (R3) efforts, university settings provide unique advantages: they contain millions of young adults who are developmentally primed to explore new activities, and they cultivate a social atmosphere where new identities can flourish. From 2018 to 2020, we surveyed 17,203 undergraduate students at public universities across 22 states in the United States to explore R3 potential on college campuses and assess key demographic, social, and cognitive correlates of past and intended future hunting behavior. After weighting to account for demographic differences between our sample and the larger student population, 29% of students across all states had hunted in the past. Students with previous hunting experience were likely to be white, male, from rural areas or hunting families, and pursuing degrees related to natural resources. When we grouped students into 1 of 4 categories with respect to hunting (i.e., non‐hunters [50%], potential hunters [22%], active hunters [26%], and lapsed hunters [3%]), comparisons revealed differences based on demographic attributes, beliefs, attitudes, and behaviors. Compared to active hunters, potential hunters were more likely to be females or racial and ethnic minorities, and less likely to experience social support for hunting. Potential hunters valued game meat and altruistic reasons for hunting, but they faced unique constraints due to lack of hunting knowledge and skills. Findings provide insights for marketing and programming designed to achieve R3 objectives with a focus on university students. © 2021 The Wildlife Society.
American Black Duck Nesting Ecology in North Carolina
Authors: Lawson Daniel M. , Williams Christopher K. , Howell Douglas L. , Fuller Joseph C. 
Published: May 2021

ABSTRACTNorth Carolina, USA, is the southernmost extent of the American black duck's (Anas rubripes) breeding range; however, little is known about their nesting ecology in this region. We located and monitored 140 nesting black ducks over 2 years (2017–2018) to quantify preferred nesting habitat and assess nesting productivity within coastal North Carolina. We located nests in brackish marshes (75%) and man‐made dredge spoil islands (25%) at a density of 1 nest/22 ha. Black ducks selected high marsh and nested an average of 21.81 m from open water at a mean elevation of 1.36 m. In preferred nesting habitat, visual obstruction readings were 0.50 m with a maximum mean vegetation height of 0.81 m and land cover consisted largely of grasses (84.6%). Apparent nest success rates varied from 31% (2017) to 63% (2018) across years. The majority (72.2%) of variability in nest success was best predicted by nest location (mainland marsh, natural island, or spoil island), vegetation density, maximum vegetation height, and year. Management for breeding black ducks in coastal North Carolina should focus on promoting selected nesting habitat and reducing nest predators. Prescribed burns, used to set back succession on spoil islands and in brackish marshes should be conducted in the winter or in the early growing season not to exceed the twenty‐fifth quantile date of black duck nest initiation (2 Apr). © 2021 The Wildlife Society.
Introducing a data availability policy for journals at IOP Publishing: Measuring the impact on authors and editorial teams
Authors: Holt Jade , Walker Andrew , Jones Phill 
Published: May 2021

AbstractAs the open research movement continues to gather pace, a number of publishers, funders, and institutions are mandating the sharing of underlying research data. At the same time, concerns about introducing extra quality control steps around data availability statements (DAS) are driving a discussion about the best way to make data more open without slowing down publication. This article describes a pilot project to introduce a new Open Data policy to three IOP Publishing (IOPP) journals as part of IOPP's commitment to increasing transparency and support for open science. An investigation was undertaken using an automated workflow monitoring tool to understand the impact of this change on authors and the editorial staff. Changes in revised submission processing times and how often manuscripts were returned to the author were measured. An overall increase in the time editorial staff spent processing manuscripts was found as well as an increase in the number of times manuscripts were returned to authors. Detailed analysis shows that manuscripts in which authors claim in the DAS to have included data within the manuscript were the most strongly affected. Steps to mitigate the effects through improved author communication were found to be effective.
Segmented simultaneous multi‐slice diffusion‐weighted imaging with navigated 3D rigid motion correction
Authors: Riedel (né Steinhoff) Malte , Setsompop Kawin , Mertins Alfred , Börnert Peter 
Published: May 2021

PurposeTo improve the robustness of diffusion‐weighted imaging (DWI) data acquired with segmented simultaneous multi‐slice (SMS) echo‐planar imaging (EPI) against in‐plane and through‐plane rigid motion.Theory and MethodsThe proposed algorithm incorporates a 3D rigid motion correction and wavelet denoising into the image reconstruction of segmented SMS‐EPI diffusion data. Low‐resolution navigators are used to estimate shot‐specific diffusion phase corruptions and 3D rigid motion parameters through SMS‐to‐volume registration. The shot‐wise rigid motion and phase parameters are integrated into a SENSE‐based full‐volume reconstruction for each diffusion direction. The algorithm is compared to a navigated SMS reconstruction without gross motion correction in simulations and in vivo studies with four‐fold interleaved 3‐SMS diffusion tensor acquisitions.ResultsSimulations demonstrate high fidelity was achieved in the SMS‐to‐volume registration, with submillimeter registration errors and improved image reconstruction quality. In vivo experiments validate successful artifact reduction in 3D motion‐compromised in vivo scans with a temporal motion resolution of approximately 0.3 s.ConclusionThis work demonstrates the feasibility of retrospective 3D rigid motion correction from shot navigators for segmented SMS DWI.
A model‐based framework for correcting inhomogeneity effects in magnetization transfer saturation and inhomogeneous magnetization transfer saturation maps
Authors: Rowley Christopher D. , Campbell Jennifer S. W. , Wu Zhe , Leppert Ilana R. , Rudko David A. , Pike Gilbert Bruce , Tardif Christine L. 
Published: May 2021

PurposeIn this work, we propose that Δ‐induced errors in magnetization transfer (MT) saturation (MTsat) maps can be corrected with use of an R1 and map and through numerical simulations of the sequence.Theory and MethodsOne healthy subject was scanned at 3.0T using a partial quantitative MT protocol to estimate the relationship between observed R1 (R1,obs) and apparent bound pool size () in the brain. MTsat values were simulated for a range of , R1,obs, and . An equation was fit to the simulated MTsat, then a linear relationship between R1,obs and was generated. These results were used to generate correction factor maps for the MTsat acquired from single‐point data. The proposed correction was compared to an empirical correction factor with different MT‐preparation schemes.Results was highly correlated with R1,obs (r > 0.96), permitting the use of R1,obs to estimate for correction. All corrected MTsat maps displayed a decreased correlation with compared to uncorrected MTsat and MTsat corrected with an empirical factor in the corpus callosum. There was good agreement between the proposed approach and the empirical correction with radiofrequency saturation at 2 kHz, with larger deviations seen when using saturation pulses further off‐resonance and in inhomogeneous (ih) MTsat maps.ConclusionThe proposed correction decreases the dependence of MTsat on inhomogeneities. Furthermore, this flexible framework permits the use of different saturation protocols, making it useful for correcting inhomogeneities in ihMT.
An innovative operation‐time‐space network for solving different logistic problems with capacity and time constraints
Authors: Ambrosino Daniela , Asta Veronica 
Published: May 2021

AbstractThis work describes an innovative operation‐time‐space network that can be easily adapted to model different logistic problems involving time and space decisions. In the recent literature, different time‐space networks have been proposed to deal with specific requirements of problems arising in the logistic and transportation fields. The proposed network is able to deal with different types of capacity and time constraints that characterized the most part of logistic problems. The article explains how to construct the operation‐time‐space network, how to model time and capacity constraints of different types; moreover, it presents an application of this network to solve a logistic problem arising in the rail‐sea exchange node, that is the scheduling of port rail shunting operations. Other applications are briefly introduced. Finally, the well‐known quay crane scheduling problem is modeled, thanks to the operation‐time‐space network; the related flow model is tested by using a benchmark suite available in the literature.
Influence of solvent, Lewis acid–base complex, and nanoparticles on the morphology and gas separation properties of polysulfone membranes
Authors: Maghami Saeid , Sadeghi Morteza , Baghersad Samaneh , Zornoza Beatriz 
Published: May 2021

AbstractA series of polysulfone (PSF) membranes were prepared using different solvents: dimethylformamide (DMF), tetrahydrofuran, dimethylacetamide, and n‐methyl‐2‐pyrrolidone (NMP). The PSF membrane prepared by NMP showed the highest gas permeability. The influence of propionic acid as a Lewis acid on gas separation properties of the PSF was explored. The PSF membrane prepared by the casting solution containing 25 wt% PSF, 35 wt% propionic acid, and 40 wt% NMP showed a superior gas separation performance. The gas permeation measurements indicated that incorporating 30 wt% γ‐alumina nanoparticles into the PSF matrix resulted in about the respective 43% and 41% increase in CO2 and O2 permeability together with a rise in CO2/CH4 and O2/N2 selectivities (13% and 7%, respectively). Furthermore, by rearranged modified Maxwell model, the role and nature of the interfacial layer in the PSF‐based mixed matrix membranes were mathematically analyzed considering a reduced permeability factor.