bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated proteomic and metabolomic analyses reveal the importance of aroma precursor accumulation and storage in methyl jasmonate-primed tea leaves
Authors: Shi Jiang , Wang Jiatong , Lv Haipeng , Peng Qunhua , Schreiner Monika , Baldermann Susanne , Lin Zhi 
Published: May 2021

AbstractIn response to preharvest priming with exogenous methyl jasmonate (MeJA), tea plants adjust their physiological behavior at the molecular level. The whole-organism reconfiguration of aroma formation from the precursor to storage is poorly understood. In this study, we performed iTRAQ proteomic analysis and identified 337, 246, and 413 differentially expressed proteins in tea leaves primed with MeJA for 12 h, 24 h, and 48 h, respectively. Furthermore, a total of 266 nonvolatile and 100 volatile differential metabolites were identified by utilizing MS-based metabolomics. A novel approach that incorporated the integration of extended self-organizing map-based dimensionality was applied. The vivid time-scale changes tracing physiological responses in MeJA-primed tea leaves are marked in these maps. Jasmonates responded quickly to the activation of the jasmonic acid pathway in tea leaves, while hydroxyl and glycosyl jasmonates were biosynthesized simultaneously on a massive scale to compensate for the exhausted defense. The levels of α-linolenic acid, geranyl diphosphate, farnesyl diphosphate, geranylgeranyl diphosphate, and phenylalanine, which are crucial aroma precursors, were found to be significantly changed in MeJA-primed tea leaves. Green leaf volatiles, volatile terpenoids, and volatile phenylpropanoids/benzenoids were spontaneously biosynthesized from responding precursors and subsequently converted to their corresponding glycosidic forms, which can be stably stored in tea leaves. This study elucidated the physiological response of tea leaves primed with exogenous methyl jasmonate and revealed the molecular basis of source and sink changes on tea aroma biosynthesis and catabolism in response to exogenous stimuli. The results significantly enhance our comprehensive understanding of tea plant responses to exogenous treatment and will lead to the development of promising biotechnologies to improve fresh tea leaf quality.
Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens
Authors: Peng Ze , He Yanhong , Parajuli Saroj , You Qian , Wang Weining , Bhattarai Krishna , Palmateer Aaron J. , Deng Zhanao 
Published: May 2021

AbstractDowny mildew (DM), caused by obligate parasitic oomycetes, is a destructive disease for a wide range of crops worldwide. Recent outbreaks of impatiens downy mildew (IDM) in many countries have caused huge economic losses. A system to reveal plant–pathogen interactions in the early stage of infection and quickly assess resistance/susceptibility of plants to DM is desired. In this study, we established an early and rapid system to achieve these goals using impatiens as a model. Thirty-two cultivars ofImpatiens wallerianaandI. hawkeriwere evaluated for their responses to IDM at cotyledon, first/second pair of true leaf, and mature plant stages. AllI. wallerianacultivars were highly susceptible to IDM. While allI. hawkericultivars were resistant to IDM starting at the first true leaf stage, many (14/16) were susceptible to IDM at the cotyledon stage. Two cultivars showed resistance even at the cotyledon stage. Histological characterization showed that the resistance mechanism of theI. hawkericultivars resembles that in grapevine and type II resistance in sunflower. By integrating full-length transcriptome sequencing (Iso-Seq) and RNA-Seq, we constructed the first reference transcriptome forImpatienscomprised of 48,758 sequences with an N50 length of 2060 bp. Comparative transcriptome and qRT-PCR analyses revealed strong candidate genes for IDM resistance, including three resistance genes orthologous to the sunflower geneRGC203, a potential candidate associated with DM resistance. Our approach of integrating early disease-resistance phenotyping, histological characterization, and transcriptome analysis lay a solid foundation to improve DM resistance in impatiens and may provide a model for other crops.
Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry
Authors: Dong Xiangxiang , Li Yanjun , Guan Yuhan , Wang Shaoxi , Luo He , Li Xiaoming , Li He , Zhang Zhihong 
Published: May 2021

AbstractFlowering time is known to be regulated by numerous pathways, such as the autonomous, gibberellin, aging, photoperiod-mediated, and vernalization pathways. These regulatory mechanisms involve both environmental triggers and endogenous hormonal cues. Additional flowering control mechanisms mediated by other phytohormones, such as auxin, are less well understood. We found that in cultivated strawberry (Fragaria × ananassa), the expression of auxin response factor4 (FaARF4) was higher in the flowering stage than in the vegetative stage. Overexpression of FaARF4 in Arabidopsis thaliana and woodland strawberry (Fragaria vesca) resulted in transgenic plants flowering earlier than control plants. In addition, FveARF4-silenced strawberry plants showed delayed flowering compared to control plants, indicating that FaARF4 and FveARF4 function similarly in regulating flowering. Further studies showed that ARF4 can bind to the promoters of the floral meristem identity genes APETALA1 (AP1) and FRUITFULL (FUL), inducing their expression and, consequently, flowering in woodland strawberry. Our studies reveal an auxin-mediated flowering pathway in strawberry involving the induction of ARF4 expression.
A nondestructive Bell-state measurement on two distant atomic qubits
Authors: Welte Stephan , Thomas Philip , Hartung Lukas , Daiss Severin , Langenfeld Stefan , Morin Olivier , Rempe Gerhard , Distante Emanuele 
Published: May 2021

AbstractOne of the most fascinating aspects of quantum networks is their capability to distribute entanglement as a nonlocal communication resource1. In a first step, this requires network-ready devices that can generate and store entangled states2. Another crucial step, however, is to develop measurement techniques that allow for entanglement detection. Demonstrations for different platforms3–13 suffer from being not complete, destructive or local. Here, we demonstrate a complete and nondestructive measurement scheme14–16 that always projects any initial state of two spatially separated network nodes onto a maximally entangled state. Each node consists of an atom trapped inside an optical resonator from which two photons are successively reflected. Polarization measurements on the photons discriminate between the four maximally entangled states. Remarkably, such states are not destroyed by our measurement. In the future, our technique might serve to probe the decay of entanglement and to stabilize it against dephasing via repeated measurements17,18.
Endodontic length measurements using cone beam computed tomography with dedicated or conventional software at different voxel sizes
Authors: Van Pham Khoa 
Published: May 2021

AbstractThe aim of this study is to investigate the accuracies and the agreements of the 3D Endo software, conventional CBCT software Romexis Viewer at three voxel sizes, and the EAL ProPex Pixi in endodontic length measurements. Three hundred and twenty-nine root canals in 120 intact human extracted molars were accessed. The actual lengths (AL) and electronic lengths (EL) were measured using the ruler and electronic apex locator (EAL), respectively. Teeth were scanned using the CBCT at different voxel sizes (0.075, 0.10, and 0.15 mm). Root canal lengths were measured using 3D Endo with proposed length (3D-PL) by software, corrected length (3D-CL), Romexis Viewer. The Fisher’s exact test, paired t-test and Bland–Altman plots were calculated to detect the agreements of the four methods with AL measurements. The ProPex Pixi measurements obtained the highest accuracy in the range of ± 0.5 mm. There was agreement between the 3D-PL and the 3D-CL with AL measurements at voxel size of 0.15 mm and at voxel size of 0.10 mm, respectively. The CBCT Romexis Viewer measurements agreed with AL at three voxel sizes. The conventional CBCT measurements using Romexis Viewer and dedicated software did not reach to the 100% accuracy in the range of ± 0.5 mm.
Worker ants promote outbreeding by transporting young queens to alien nests
Authors: Vidal Mathilde , Königseder Florian , Giehr Julia , Schrempf Alexandra , Lucas Christophe , Heinze Jürgen 
Published: May 2021

AbstractChoosing the right mating partner is one of the most critical decisions in the life of a sexually reproducing organism and is the basis of sexual selection. This choice is usually assumed to be made by one or both of the sexual partners. Here, we describe a system in which a third party – the siblings – promote outbreeding by their sisters: workers of the tiny ant Cardiocondyla elegans carry female sexuals from their natal nest over several meters and drop them in the nest of another, unrelated colony to promote outbreeding with wingless, stationary males. Workers appear to choose particular recipient colonies into which they transfer numerous female sexuals. Assisted outbreeding and indirect female choice in the ant C. elegans are comparable to human matchmaking and suggest a hitherto unknown aspect of natural history – third party sexual selection. Our study highlights that research at the intersection between social evolution and reproductive biology might reveal surprising facets of animal behavior.
Environmental DNA provides higher resolution assessment of riverine biodiversity and ecosystem function via spatio-temporal nestedness and turnover partitioning
Authors: Seymour Mathew , Edwards François K. , Cosby Bernard J. , Bista Iliana , Scarlett Peter M. , Brailsford Francesca L. , Glanville Helen C. , de Bruyn Mark , Carvalho Gary R. , Creer Simon 
Published: May 2021

AbstractRapidly assessing biodiversity is essential for environmental monitoring; however, traditional approaches are limited in the scope needed for most ecological systems. Environmental DNA (eDNA) based assessment offers enhanced scope for assessing biodiversity, while also increasing sampling efficiency and reducing processing time, compared to traditional methods. Here we investigated the effects of landuse and seasonality on headwater community richness and functional diversity, via spatio-temporal dynamics, using both eDNA and traditional sampling. We found that eDNA provided greater resolution in assessing biodiversity dynamics in time and space, compared to traditional sampling. Community richness was seasonally linked, peaking in spring and summer, with temporal turnover having a greater effect on community composition compared to localized nestedness. Overall, our assessment of ecosystem function shows that community formation is driven by regional resource availability, implying regional management requirements should be considered. Our findings show that eDNA based ecological assessment is a powerful, rapid and effective assessment strategy that enables complex spatio-temporal studies of community diversity and ecosystem function, previously infeasible using traditional methods.
Low frequency variants associated with leukocyte telomere length in the Singapore Chinese population
Authors: Chang Xuling , Gurung Resham L. , Wang Ling , Jin Aizhen , Li Zheng , Wang Renwei , Beckman Kenneth B. , Adams-Haduch Jennifer , Meah Wee Yang , Sim Kar Seng , Lim Weng Khong , Davila Sonia , Tan Patrick , Teo Jing Xian , Yeo Khung Keong , M. Yiamunaa , Liu Sylvia , Lim Su Chi , Liu Jianjun , van Dam Rob M. , Friedlander Yechiel , Koh Woon-Puay , Yuan Jian-Min , Khor Chiea Chuen , Heng Chew-Kiat , Dorajoo Rajkumar 
Published: May 2021

AbstractThe role of low frequency variants associated with telomere length homeostasis in chronic diseases and mortalities is relatively understudied in the East-Asian population. Here we evaluated low frequency variants, including 1,915,154 Asian specific variants, for leukocyte telomere length (LTL) associations among 25,533 Singapore Chinese samples. Three East Asian specific variants in/near POT1, TERF1 and STN1 genes are associated with LTL (Meta-analysis P 2.49×10−14–6.94×10−10). Rs79314063, a missense variant (p.Asp410His) at POT1, shows effect 5.3 fold higher and independent of a previous common index SNP. TERF1 (rs79617270) and STN1 (rs139620151) are linked to LTL-associated common index SNPs at these loci. Rs79617270 is associated with cancer mortality [HR95%CI = 1.544 (1.173, 2.032), PAdj = 0.018] and 4.76% of the association between the rs79617270 and colon cancer is mediated through LTL. Overall, genetically determined LTL is particularly associated with lung adenocarcinoma [HR95%CI = 1.123 (1.051, 1.201), Padj = 0.007]. Ethnicity-specific low frequency variants may affect LTL homeostasis and associate with certain cancers.
Heavy-element Rydberg transition line emission from the post-giant-evolution star HD 101584
Authors: Olofsson H. , Black J. H. , Khouri T. , Vlemmings W. H. T. , Humphreys E. M. L. , Lindqvist M. , Maercker M. , Nyman L. , Ramstedt S. , Tafoya D. 
Published: May 2021

Context. We report the detection of two lines at millimetre wavelengths towards the immediate surroundings of the post-giant and most likely post-common-envelope star HD 101584 using high-angular-resolution ALMA observations. The circumstellar environment of this object is rich in different molecular species, but we find no viable identifications in terms of molecular lines.
Aims. We aim to determine whether or not these lines can be attributed to the Rydberg transitions – X30α and X26α – of neutral atoms of elements heavier than carbon.
Methods. A simple model in strict local thermodynamic equilibrium for a warm-gas environment of the moderate-temperature star (Teff ≈ 8500 K) was constructed to corroborate our findings. A geometrically thin, disc-like geometry seen face-on was chosen and a distance of 1 kpc.
Results. The observed flux densities of the lines and the continuum at 232 and 354 GHz can be reproduced using ≈10−3 M⊙ of gas at a temperature of ≈2800 K and a hydrogen density of ≈1012 cm−3, assuming solar abundances for the elements. The gas lies within a distance of about 5 au from the star (assuming a distance of 1 kpc). The ionisation fraction is low, ≈3 × 10−5. The origin of such a region is not clear, but it may be related to a common-envelope-evolution phase. With these conditions, the line emissions are dominated by Rydberg transitions within the stable isotopes of Mg. A turbulent velocity field in the range 5.5–7.5 km s−1 is required to fit the Gaussian line shapes. An upper limit to the average magnetic field in the line-emitting region of 1 G is set using the Zeeman effect in these lines.
Conclusions. We speculate that Rydberg transitions of heavy elements may be an interesting probe for the close-in environments of other moderate-temperature objects like AGB stars, red supergiants, yellow hypergiants, and binaries of various types.
miR-204-5p and Platelet Function Regulation: Insight into a Mechanism Mediated by CDC42 and GPIIbIIIa
Authors: Garcia Alix , Dunoyer-Geindre Sylvie , Nolli Séverine , Strassel Catherine , Reny Jean-Luc , Fontana Pierre 
Published: May 2021

Abstract
Background Several platelet-derived microRNAs are associated with platelet reactivity (PR) and clinical outcome in cardiovascular patients. We previously showed an association between miR-204-5p and PR in stable cardiovascular patients, but data on functional mechanisms are lacking.
Aims To validate miR-204-5p as a regulator of PR in platelet-like structures (PLS) derived from human megakaryocytes and to address mechanistic issues.
Methods Human hematopoietic stem cells were differentiated into megakaryocytes, enabling the transfection of miR-204-5p and the recovery of subsequent PLS. The morphology of transfected megakaryocytes and PLS was characterized using flow cytometry and microscopy. The functional impact of miR-204-5p was assessed using a flow assay, the quantification of the activated form of the GPIIbIIIa receptor, and a fibrinogen-binding assay. Quantitative polymerase chain reaction and western blot were used to evaluate the impact of miR-204-5p on a validated target, CDC42. The impact of CDC42 modulation was investigated using a silencing strategy.
Results miR-204-5p transfection induced cytoskeletal changes in megakaryocytes associated with the retracted protrusion of proPLS, but it had no impact on the number of PLS released. Functional assays showed that the PLS produced by megakaryocytes transfected with miR-204-5p were more reactive than controls. This phenotype is mediated by the regulation of GPIIbIIIa expression, a key contributor in platelet–fibrinogen interaction. Similar results were obtained after CDC42 silencing, suggesting that miR-204-5p regulates PR, at least in part, via CDC42 downregulation.
Conclusion We functionally validated miR-204-5p as a regulator of the PR that occurs through CDC42 downregulation and regulation of fibrinogen receptor expression.
Gross Total Resection of a Recurrent Cavernous Sinus Meningioma through a Combined Transzygomatic Transcavernous and Extended Middle Fossa Approach with Cavernous Carotid Denudation
Authors: Nonaka Yoichi , Hayashi Naokazu , Fukushima Takanori 
Published: May 2021

Abstract
Objective The aim of this study is to describe surgical management of invasive cavernous sinus meningioma with a combination of skull base approaches.
Design This study is an operative video.
Results Resection of the recurrent skull base meningioma is still challenging, especially if the tumor involves or encases the carotid artery. In this video, we describe our experience with the successful treatment of a recurrent skull base meningioma, which involved the entire cavernous sinus and the internal carotid artery. A 53-year-old male presented with a 1-year history of progressing right-side complete oculomotor palsy and facial dysesthesia. The patient had previously undergone craniotomy for the right-side petroclival cavernous meningioma (Fig. 1A and B). Total 8 years after the first surgery, the remaining portion of the cavernous sinus grew up and extended into the posterior fossa (Fig. 1C). Then the second surgery was performed to resect only the posterior fossa component (Fig. 1D). However, the follow-up magnetic resonance imaging revealed an aggressive tumor regrowth in 2 years. The tumor occupied the right middle fossa with an extension to the posterior fossa and infratemporal fossa (Fig. 1E and F). We scheduled to perform gross total resection of the tumor through a combined transzygomatic transcavernous and extended middle fossa approach with preparation for vessel reconstruction. Mild adhesion between the tumor and the cavernous carotid artery facilitated complete resection of the intracavernous component of the tumor (Fig. 2A–C).
Conclusion A combination of skull base approaches provides multidirectional operative corridors and wide exposure of the skull base lesions.The link to the video can be found at https://youtu.be/DB_WXFeyBvo.
Radiological Anatomy of Stomach and Duodenum with Clinical Significance
Authors: Vora Zainab , Goyal Ankur , Sharma Raju 
Published: May 2021

AbstractAnatomy is the key to accurate imaging interpretation. It is essential for radiologists to thoroughly understand the normal anatomy and spatial relationships of the stomach and duodenum to accurately localize the site of abnormality. In this article, we describe in detail the gross and applied radiological anatomy of the stomach and duodenum, and the current role of various radiological investigations (including barium studies, endoscopic ultrasonography, computed tomography, and magnetic resonance imaging) in the evaluation of the diseases involving the stomach and duodenum.
The Time for Parenteral Nutrition is Now: 12 Months Caring for Patients with Severe COVID-19
Authors: Barbosa Claudio de Lima 
Published: May 2021

AbstractThe coronavirus disease 2019 (COVID-19) pandemic has brought new challenges to adequate nutrition, especially in critically ill patients. Physicians caring for these patients face great difficulties, including a different pathophysiology compared with other diseases, inadequately trained personnel, pressure from the media and family members, logistical and economic obstacles, and lack of robust medical evidence. Although the literature on COVID-19 is still limited, evidence demonstrates the need to reevaluate the use of effective nutritional support in this unprecedented, challenging clinical context. Supplemental parenteral nutrition must be considered due to the energy expenditure from COVID-19, the difficulty in achieving protein and energy goals in patients under enteral nutrition in prone position, and the worsened gastroparesis related to high doses of sedative/neuromuscular blocking agents.
Midtrimester Cervical Length in Low-Risk Nulliparous Women for the Prediction of Spontaneous Preterm Birth: Should We Consider a New Definition of Short Cervix?
Authors: Guerby Paul , Girard Mario , Marcoux Geneviève , Beaudoin Annie , Pasquier Jean-Charles , Bujold Emmanuel 
Published: May 2021

Objective The study aimed to estimate the predictive value of midtrimester cervical length (CL) and the optimal cut-off of CL that should be applied with asymptomatic nulliparous women for the prediction of spontaneous preterm birth (sPTB).
Study Design This is a prospective cohort study of asymptomatic nulliparous women with a singleton gestation. Participants underwent CL measurement by transvaginal ultrasound between 20 and 24 weeks of gestation. The participants and their health care providers remained blinded to the results of CL measurement. The primary outcomes were sPTB before 35 weeks and sPTB before 37 weeks. Receiver operating characteristics (ROC) curve analyses were performed. Analyses were repeated by using multiples of median (MoM) of CL adjusted for gestational age.
Results Of 796 participants, the mean midtrimester CL was 40 ± 6 mm with a 1st, 5th, and 10th percentile of 25, 29, and 32 mm, respectively. ROC curve analyses suggest that a cut-off of 30 mm was the optimal CL to predict sPTB before 35 weeks (area under the ROC curve [AUC]: 0.70, 95% confidence interval [CI]: 0.56–0.85) and before 37 weeks (AUC: 0.70, 95% CI: 0.59–0.80). Midtrimester CL <30 mm could detect 35% of all sPTB before 35 weeks at a false-positive rate of 5% (relative risk: 9.1, 95% CI: 3.5–23.5, p < 0.001). We observed similar results using a cut-off of CL <0.75 MoM adjusted for gestational age.
Conclusion A midtrimester CL cut-off of 30 mm (instead of 25 mm), or CL less than 0.75 MoM, should be used to identify nulliparous women at high risk of sPTB.
Key Points