bluuebunny/crossref_metadata_2025_split huggingface dataset:
BBX16 mediates the repression of seedling photomorphogenesis downstream of the GUN1-GLK1 module during retrograde signaling
Authors: Veciana Nil , Martín Guiomar , Leivar Pablo , Monte Elena 
Published: November 2021

SummaryPlastid-to-nucleus retrograde signals (RS) initiated by dysfunctional chloroplasts impact photomorphogenic development. We previously showed that the transcription factor GLK1 acts downstream of the RS-regulator GUN1 in photodamaging conditions to regulate not only the well-established expression of photosynthesis-associated nuclear genes (PhANGs) but also to regulate seedling morphogenesis. Specifically, the GUN1/GLK1 module inhibits the light-induced PIF-repressed transcriptional network to suppress cotyledon development when chloroplast integrity is compromised, modulating the area exposed to potentially damaging high light. However, how the GUN1/GLK1 module inhibits photomorphogenesis upon chloroplast damage remained undefined.Here, we report the identification of BBX16 as a novel direct target of GLK1. BBX16 is induced and promotes photomorphogenesis in moderate light and it is repressed via GUN1/GLK1 after chloroplast damage. Additionally, we show that BBX16 represents a regulatory branching point downstream of GUN1/GLK1 in the regulation of PhANG expression and seedling development upon RS activation.The gun1 phenotype in lincomycin and the gun1-like phenotype of GLK1OX are markedly suppressed in gun1bbx16 and GLK1OXbbx16.This study identifies BBX16 as the first member of the BBX family involved in RS, and defines a molecular bifurcation mechanism operated by GLK1/BBX16 to optimize seedling deetiolation, and to ensure photoprotection in unfavorable light conditions.
Developmental dynamics of the neural crest-mesenchymal axis in creating the thymic microenvironment
Authors: Handel Adam E. , Cheuk Stanley , Dhalla Fatima , Maio Stefano , Hübscher Tania , Rota Ioanna , Deadman Mary E. , Ekwall Olov , Lütolf Matthias , Weinberg Kenneth , Holländer Georg 
Published: November 2021

AbstractThe thymic stroma is composed of epithelial and non-epithelial cells that collectively provide separate microenvironments controlling the homing of blood-born precursors to the tissue, and their subsequent differentiation to functionally mature and correctly selected T cells. While thymic epithelial cells are well characterized for their role in thymopoiesis, a comparably comprehensive analysis of the non-epithelial thymic stroma is lacking. Here we explore at single cell resolution the complex composition and dynamic changes that occur over time in the non-epithelial stromal compartment. We detail across different developmental stages in human and mouse thymus, and in an experimental model of Di George syndrome, the most common form of human thymic hypoplasia, the separate transcriptomes of mouse mesothelium, fibroblasts, neural crest cells, endothelial and vascular mural cells. The detected gene expression signatures identify novel stromal subtypes and relate their individual molecular profiles to separate differentiation trajectories and functions. Specifically, we demonstrate an abundance and unprecedented heterogeneity of diverse fibroblast subtypes that emerge at discrete developmental stages and vary in their expression of key regulatory signalling circuits and components of the extracellular matrix. Taken together, these findings highlight the dynamic complexity of the non-epithelial thymus stroma and link the cells’ specific gene expression profiles to separate instructive roles essential for normal thymus organogenesis and tissue maintenance.TeaserSingle cell profiling of thymic stroma identifies a dynamic contribution from neural crest cells to the thymic mesenchyme.
ETV2 regulates PARP-1 binding protein to induce ER stress-mediated cell death in tuberin-deficient cells
Authors: Shrestha Shikshya , Lamattina Anthony , Pacheco-Rodriguez Gustavo , Ng Julie , Liu Xiaoli , Sonawane Abhijeet , Imani Jewel , Qiu Weiliang , Kosmas Kosmas , Louis Pierce , Hentschel Anne , Steagall Wendy K. , Onishi Rieko , Christou Helen , Henske Elizabeth P. , Glass Kimberly , Perrella Mark A. , Moss Joel , Tantisira Kelan , El-Chemaly Souheil 
Published: November 2021

AbstractLymphangioleiomyomatosis (LAM) is a rare progressive disease, characterized by mutations in the tuberous sclerosis complex genes (Tsc1 or Tsc2), and hyperactivation of mechanistic target of rapamycin complex 1 (mTORC1). The effectiveness of mTORC1 inhibitors is limited by their lack of cytotoxic effects. Here, we report that E26 transformation specific (ETS) Variant Transcription Factor 2 (ETV2) is a critical regulator of Tsc2-deficient cell survival. Nuclear localization of ETV2 in Tsc2-deficient cells is mTORC1-independent and is enhanced by spleen tyrosine kinase (Syk) inhibition. In the nucleus, ETV2 transcriptionally regulates poly(ADP-ribose) polymerase 1 binding protein (PARPBP), a coregulator of transcription, mRNA and protein expression. Silencing of ETV2 or PARPBP in Tsc2-deficient cells induced ER-stress and increased cell deathin vitroandin vivo. We also found ETV2 expression in human cells with loss of heterozygosity forTSC2lending support to the translational relevance of our findings. In conclusion, we report a novel signaling axis unique to Syk-inhibition is mTORC1-independent and promotes a cytocidal response in Tsc2-deficient cells, and therefore, maybe a potential alternative therapeutic target in LAM.
Energy metabolism adaptations and gene expression reprogramming in a cellular MAFLD model
Authors: Zhou Tianran , Cömert Cagla , Zhou Xiaoyu , Lin Lin , Bolund Lars , Palmfeldt Johan , Tong Guangdong , Luo Yonglun , Bross Peter 
Published: November 2021

AbstractMitochondrial dysfunction plays a critical role in metabolic associated fatty liver disease (MAFLD). This study aims to characterize mitochondrial dysfunctions in a human MAFLD Huh7 cell model triggered by free fatty acid (FFA) (palmitate and oleate) overload for 24 hours. We investigate its impact on cellular energy metabolism and identify potential targets for MAFLD treatment. FFA-treated cells displayed an accumulation of lipid droplets and slightly decreased viability but no significant changes in mitochondrial superoxide levels. Bioenergetic analysis showed a shift to more respiration and less glycolytic fermentation. Comprehensive transcriptomics and proteomics analyses identified changes in the expression of genes prominently involved in fatty acid handling and metabolism. The expressions of seven genes were consistently and significantly (p < 0.05) altered (4 upregulated and 3 downregulated genes) in both proteomics and transcriptomics. The FFA-treated Huh7 cell model is an appropriate in vitro model to study fatty acid metabolism and suitable to investigate the role of mitochondria, glycolysis, and multiple metabolic pathways in MAFLD. Our comprehensive analyses form a basis for drug discovery and screening using this model.
A simple and general approach to control the activity of DNA processing enzymes
Authors: Kesici Merve-Zeynep , Tinnefeld Philip , Vera Andrés Manuel 
Published: November 2021

ABSTRACTDNA processing enzymes, such as DNA polymerases and endonucleases, have found many applications in biotechnology, molecular diagnostics, and synthetic biology, among others. The development of enzymes with controllable activity, such as hot-start or light-activatable versions, has boosted their applications and improved the sensitivity and specificity of the existing ones. However, current approaches to produce controllable enzymes are experimentally demanding to develop and case specific. Here, we introduce a simple and general method to design light-start DNA processing enzymes. In order to prove its versatility, we applied our method to three DNA polymerases commonly used in biotechnology, including the Phi29 (mesophilic), Taq and Pfu polymerases, and one restriction enzyme. Light-start enzymes showed suppressed polymerase, exonuclease and endonuclease activity until they were re-activated by an UV pulse. Finally, we applied our enzymes to common molecular biology assays, and showed comparable performance to commercial hot-start enzymes.
The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone
Authors: Li Xin , Singh Noor , Miller Camille , Washington India , Sosseh Bintou , Gordon Kacy Lynn 
Published: November 2021

AbstractThe C. elegans adult hermaphrodite germ line is surrounded by a thin tube formed by somatic sheath cells that support germ cells as they mature from the stem-like mitotic state through meiosis, gametogenesis and ovulation. Recently, we discovered that the distal Sh1 sheath cells associate with mitotic germ cells as they exit the niche (Gordon et al., 2020). Here we report that these sheath-associated germ cells differentiate first in animals with temperature-sensitive mutations affecting germ cell state, and stem-like germ cells are maintained distal to the Sh1 boundary. We analyze several markers of the distal sheath, which is best visualized with endogenously tagged membrane proteins, as overexpressed fluorescent proteins fail to localize to distal membrane processes and can cause gonad morphology defects. However, such reagents with highly variable expression can be used to determine the relative positions of the two Sh1 cells, one of which often extends further distal than the other.
Model predictive control of direct-driven surface-mounted permanent magnet synchronous generator based on active disturbance rejection controller
Authors: Shi Hongjun , Xiong Lei , Nie Xuchen , Zhu Qixin 
Published: November 2021

Purpose
This paper aims to mainly discuss how to suppress the disturbances accurately and effectively in the wind energy conversion system (WECS) of the direct drive surface mount permanent magnet synchronous generator (SPMSG).

Design/methodology/approach
The disturbances in wind energy conversion system have seriously negative influence on the maximum power tracking performance. Therefore, a model predictive control (MPC) method of model compensation active disturbance rejection control (ADRC) strategy in parallel connection is designed, which optimizes the speed tracking performance compared with the existing control strategy of MPC and ADRC in series connection. Based on the traditional ADRC, a multi parameter model compensation ADRC strategy is added to better estimate the disturbances. At the same time, a torque feedback strategy is added to compensate the disturbances caused by load torque and further optimize the speed loop tracking performance.

Findings
The simulation results show that the designed control method has advantages than the traditional control method in compensating the disturbances and tracking the maximum power more effectively.

Originality/value
The simulation results show that the designed control method is superior to the traditional proportional control method, which can better compensate the internal and external disturbances and track the maximum power more effectively.
Socio-economic and technological new normal in supply chain management: lessons from COVID-19 pandemic
Authors: Ajmal Mian M. , Khan Mehmood , Shad Muhammad Kashif , AlKatheeri Haseena , Jabeen Fauzia 
Published: November 2021

PurposeThis paper explores the new normal activities and strategic responses of the service industry towards the challenges created by the coronavirus disease 2019 (COVID-19) outbreak and other constructs and validates the measurement scale for socio-economic and technological new normal activities following lockdown and social distancing practices.Design/methodology/approachFirst, structured interviews with 28 participants helped us generate items and develop survey instruments for cross-sectional data collection in the second phase. So, the authors received 256 complete responses from the top and middle management of the services industry. Exploratory factor analysis helped us explore the factors and reliability of the items. Confirmatory factor analysis aided us in generating and confirming the factorial structure of the constructs.FindingsResults indicated that amid COVID-19's pandemic, new normal activities are emerging in which organizations are deploying crisis strategies to safeguard their business and stakeholders. Organizations are re-opening swiftly, focusing on digital transformation, developing digital platforms for ease in working and improved consumer services, to name a few operational changes.Practical implicationsDiscussion on empirical analysis revolves around the guidelines to service industry's managers and top management to improve shortcomings in combating the challenges they face in their operations.Originality/valuePrior studies have provided substantial insights on the COVID-19 pandemic, but relatively little research exists on new normal activities in the supply chain network of the service industry. Among other reasons for such less empirical evidence on new normal activities is the unavailability of a comprehensive tool for measuring the socio-economic and technological new normal activities. This paper is a contribution to bridging this knowledge gap.
Cannot keep up with the Joneses: how relative deprivation pushes internal migration in Austria
Authors: Jestl Stefan , Moser Mathias , Raggl Anna Katharina 
Published: November 2021

PurposeUsing aggregated data at the municipality level allows the authors to assess the role of relative deprivation (RD) – a measure of income inequality – on top of absolute income in shaping internal migration in Austria.Design/methodology/approachIn this study, the authors analyse the effect of regional income inequality on emigration rates of Austrian municipalities using a unique spatial dataset that is constructed based on Austrian administrative register data. The register-based data contain information on the municipality of residence of all individuals aged 16 and over that have their main residency in Austria, as well as their income and socio-demographic characteristics.FindingsThe authors find that increases in relative deprivation in a municipality are related to higher emigration from the municipality. Allowing for heterogeneous effects across income, education and age groups reveals that the effect is stronger among those with comparably low levels of income and among low-skilled and young individuals.Originality/valueThe unique spatially disaggregated perspective is based on novel data from Austrian administrative registers, which comprehensively capture the economic situation and geographic movements of the whole Austrian population. Most importantly, this approach allows for measuring income inequality within local communities and for a direct identification of social groups that are more sensitive to inequality.
Attention and behaviour on fashion retail websites: an eye-tracking study
Authors: Boardman Rosy , Mccormick Helen 
Published: November 2021

PurposeThe purpose of this paper is to identify attention, cognitive and affective responses towards a fashion retailer's website and the behavioural outcomes when shopping online.Design/methodology/approach52 eye-tracking tests and 52 qualitative semi-structured interviews were conducted.FindingsConsumer attention and behaviour differ across web pages throughout the shopping journey depending on its content, function and consumers' goal. Top-down attention is more dominant than bottom-up attention when consumers are shopping online for fashion items. The product listings page was the most frequented and had the most time spent on it. Consumers enjoy browsing for products and adding them to their basket to evaluate them together later. Customisation and personalisation features are the most valued due to their ability to make the experience more convenient and enjoyable.Originality/valueThis article contributes novel findings that the content and design of the website affects attention in different ways. It demonstrates that research cannot simplify viewing patterns for fashion shopping online. The study extends the SOR framework, showing that top-down attention, when provided with personalisation and customisation features, results in approach behaviour. A lack of personalisation or customisation features results in avoidance behaviour. The complex nature of consumer attention and behaviour during their holistic shopping journey advocates the need for eye-tracking research to be conducted on a live website for ecological validity, providing a methodological contribution which can be used for future research.
Exploring destination image through online reviews: an augmented mining model using latent Dirichlet allocation combined with probabilistic hesitant fuzzy algorithm
Authors: Luo Yuyan , Tong Tao , Zhang Xiaoxu , Yang Zheng , Li Ling 
Published: November 2021

PurposeIn the era of information overload, the density of tourism information and the increasingly sophisticated information needs of consumers have created information confusion for tourists and scenic-area managers. The study aims to help scenic-area managers determine the strengths and weaknesses in the development process of scenic areas and to solve the practical problem of tourists' difficulty in quickly and accurately obtaining the destination image of a scenic area and finding a scenic area that meets their needs.Design/methodology/approachThe study uses a variety of machine learning methods, namely, the latent Dirichlet allocation (LDA) theme extraction model, term frequency-inverse document frequency (TF-IDF) weighting method and sentiment analysis. This work also incorporates probabilistic hesitant fuzzy algorithm (PHFA) in multi-attribute decision-making to form an enhanced tourism destination image mining and analysis model based on visitor expression information. The model is intended to help managers and visitors identify the strengths and weaknesses in the development of scenic areas. Jiuzhaigou is used as an example for empirical analysis.FindingsIn the study, a complete model for the mining analysis of tourism destination image was constructed, and 24,222 online reviews on Jiuzhaigou, China were analyzed in text. The results revealed a total of 10 attributes and 100 attribute elements. From the identified attributes, three negative attributes were identified, namely, crowdedness, tourism cost and accommodation environment. The study provides suggestions for tourists to select attractions and offers recommendations and improvement measures for Jiuzhaigou in terms of crowd control and post-disaster reconstruction.Originality/valuePrevious research in this area has used small sample data for qualitative analysis. Thus, the current study fills this gap in the literature by proposing a machine learning method that incorporates PHFA through the combination of the ideas of management and multi-attribute decision theory. In addition, the study considers visitors' emotions and thematic preferences from the perspective of their expressed information, based on which the tourism destination image is analyzed. Optimization strategies are provided to help managers of scenic spots in their decision-making.
Relationships among absorptive capacity, creativity and job performance: the moderating role of social media usage
Authors: Ince Huseyin , Imamoglu Salih Zeki , Turkcan Hulya 
Published: November 2021

PurposeThis paper investigates the impact of absorptive capacity (ACAP) on creativity and the effect of creativity on job performance. Further, the study investigates the moderating role of social media usage on the ACAP–creativity link and the creativity–job performance link. Accordingly, drawing on the ACAP perspective and social exchange theory (SET), the study develops a conceptual model and tests the proposed relationships.Design/methodology/approachThe paper empirically tests the developed model using 512 survey responses from research and development (R&D) employees. Data were factor analyzed, and path estimates were determined using structural equation modeling to test the hypotheses.FindingsThe results reveal that (1) individual ACAP is positively related to creativity; (2) creativity is positively associated with job performance and (3) social media usage positively moderates the relationship between individual ACAP and creativity.Originality/valueThis paper contributes to the ACAP and creativity literature by establishing a comprehensive and empirically grounded framework that enlightens the relationships between ACAP, creativity, job performance and the moderating role of social media usage.
“Once an entrepreneur, always an entrepreneur” positioning analysis of written narratives
Authors: Järvensivu Anu , von Bonsdorff Monika E. 
Published: November 2021

PurposeThe negative stereotypes concerning late-career workers are found to prevail and lead to negative circulation of narratives and actions between individuals and societies. Using the context of late-career entrepreneurs, the paper aims to find an alternative and a more positive narrative concerning late-career work by focusing on entrepreneurs and the narrative positioning related to them.Design/methodology/approachThe authors used a narrative-positioning analysis, cycling through three levels of analysis and then returning to level two, in order to study our sample of seven narratives written by Finnish late-career entrepreneurs. The authors present in detail one story-telling narrative, by Matthew, and then layer the remaining six narratives to present themes, positioning and actions surrounding being a late-career entrepreneur.FindingsA more positive narrative circulation was found, which related to the master narrative of entrepreneurs as continuing “until the end” and taking care of themselves, their enterprises and different stakeholder groups even after exiting the enterprise into so-called “retirement.” The entrepreneurs were found to actively use this positive narrative to position themselves both in the story-telling world and in their local interactions. By positioning themselves as “never ending caretakers,” the entrepreneurs gave a strong account that their reasons to continue working centered on the factors social.Research limitations/implicationsThe research findings and analysis should be interpreted in the context of the Nordic countries and especially Finland.Practical implicationsThe results of this study can inform the ways in which these “never ending caretakers” can transition into retirement and adjust to life spent in retirement.Originality/valueIn the study, entrepreneurs' written answers were analyzed with narrative-positioning analyses. An alternative story of people at work was found, and a more positive narrative circulation was constructed based on their narratives.