bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Characterization of Microsatellite Markers, Genetic Diversity and Population Structure Analysis in Sapota (Manilkara Zapota (L.) P. Royen)
Authors: Ravishankar Kundapura Venkataramana , Sathanandam Pavithra , Patil Prakash , Rekha Ajitha , Muthuvel Iyyamperumal , Patel Amrutlal , Boggala Ramesh , Shirol Adiveppa 
Published: July 2021

Abstract
Manilkara zapota (L.) P. Royen, a widely adaptable and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite markers. In order to develop genomic markers primarily for sapota, we sequenced partial genomic DNA using next generation sequencing technology on the Illumina HiSeq 2500 platform. We analyzed a total of 3.3 Gb data that were assembled into 6396224 contigs. From these contigs, 3591 simple sequence repeats were identified. Among the different type of repeats mononucleotide repeats (59.1%) were predominant followed by dinucleotide (28.6%) and trinucleotide repeats (8.2%). Primers were designed for 1285 microsatellite regions from which 30 randomly selected primers were standardized and employed for amplification in 53 genotypes of sapota. We observed 692 alleles from 30 loci with a polymorphic information content ranged from 0.85 to 0.96 with a mean of 0.9118. The probability of identity ranged from 0.002 to 0.043 with a mean of 0.012. Genetic diversity assessed by neighbour-joining and STRUCTURE assignment tests showed admixed population with 3 groups. Analysis of molecular variance revealed a significant F st value of 0.69659 indicating high genetic differentiation among the 53 genotypes. The developed microsatellites will be advantageous in assessing genetic diversity, developing linkage map and also molecular characterization of genotypes
DFF40-iRGD, As A Novel Chimeric Protein With Efficient Cytotoxic and Apoptotic Effects Against Triple-Negative Breast Cancer Cells
Authors: Amrollahi-nia Raheleh , Akbari Vajihe , Shafiee Fatemeh 
Published: July 2021

Abstract
Purpose

DNA fragmenting factor (DFF40), an endonuclease inducing irreversible apoptosis protein, is down-regulated in many types of tumor cells. iRGD is a tumor-penetrating peptide with high affinity to cancer cells overexpressing αVβ3 receptors. The aim of this study was to produce the recombinant DFF40-iRGD protein as a new molecule to selectively induce cytotoxicity in cancer cells and to evaluate its biological effects.
Methods

The three-dimensional structure of DFF40-iRGD was predicted using Modeller software and its interaction with αVβ3 receptor was evaluated by HADDOCK web-server. Recombinant DFF40 and DFF40-iRGD proteins were produced using intein fusion system in Escherichia coli BL21 (DE3). To improve the soluble expression, the inducer concentration, temperature and incubation time were optimized. After purification of DFF40 and DFF40-iRGD using chitin column, the cytotoxic and apoptotic effects of the proteins against MDA-MB-231 (αVβ3 positive) and MCF-7 (αVβ3 negative) cell lines were evaluated using cell viability assay and flow cytometric analysis.
Results

The results of molecular docking indicated the proper interaction of DFF40-iRGD with the integrin receptor comparable to iRGD. The optimum conditions of soluble expression of proteins were the induction with 0.5 mM and 0.1 mM of IPTG for DFF40 and DFF40-iRGD, respectively, at 7 ºC for 24 hours. After 48 hours of incubation, DFF40-iRGD exhibited significantly higher cytotoxic effect against MDA-MB-231 cells than MCF-7 cells as IC50 values of 0.77 and 1.64 µg/ml were found for MDA-MB-231 and MCF-7 cells, respectively. However, DFF40 cytotoxicity was not significantly different in two cell lines. Furthermore, Flow cytometry results showed that the fusion protein can induce remarkably apoptotic cell death in cancer cells.
Conclusion

in this study, DFF40-iRGD protein was produced in soluble form and its inhibitory effects on cancer cell survival and induction of apoptosis were demonstrated; therefore, it has the potential to be used as a drug candidate for targeted treatment of breast cancer, especially Triple Negative Breast Cancer Cells.
In-Vivo Study of Green Synthesised Gold Nanoparticles on Inflammatory Cytokines as Diagnostic Biomarker in Acetaminophen Induced Immunotoxicity in Rat Model
Authors: Mitra Mousumi , Mitra Sudeep , Nandi Dilip Kumar 
Published: July 2021

Abstract
Nanomedicines are widely used as possible therapeutics and diagnostics for wide variety of diseases. It have been used for successful delivery of hydrophilic and hydrophobic small molecules drugs, and biomacromolecules, such as enzymes, recombinant proteins, peptides, hormones, monoclonal antibodies and also used for the delivery of nucleic acids of different sizes and structures.Gold nanoparticles possess promising ameliorative effects due to their distinctive properties such as high surface reactivity, biocompatibility, flexibility in functionalization and a broad range of delivery targets. This study was designed to investigate the protective effect of green synthesised gold nanoparticles (AuNPs) using aqueous bark extract of Terminaliaarjunaon acetaminophen induced immunotoxicityin the experimental rat model.Group1- Control group; Group 2- Acetaminophen administered intraperitoneally at concentration of 500mg /kg of body weight for 14 days; Group 3- Co-administration of Terminaliaarjuna aqueous bark extract (175µg/kg/day) along with acetaminophen (500mg/kg/day) treatment for 14 days; Group 4- Co-administration of AuNPs (175µg/kg/day) along with acetaminophen treatment (500mg/kg/day) intraperitoneally for 14 days. After 14 days all animals were sacrificed for the immunological analysis.Immunological analysis revealed that there was significant decrease in the IL-10 level with acetaminophen treatment but marked increase in the KIM-1, Cystatin C, TNF-alpha and, IL-18 level.After co-administration with AuNPs along with acetaminophen showed effective significant recovery in theexpression of inflammatory biomarkers. Hence, the results highlighted on the protective effectsAuNPs against acetaminophen inducedimmunotoxity.
Deployment of Hub-Spoke Tethered Satellite Formation with Adaptive Sliding Mode Tension Control
Authors: Liu Chenguang , Wang Wei , Kang Junjie , Zhu Zheng H. 
Published: July 2021

Abstract
This paper studies the deployment control of a spinning hub-spoke tethered satellite formation, which is a challenging issue due to the strong nonlinear coupling between the hub and sub-satellites, and the underactuated nature of the system if no thrust is used for control. The mathematical model of the formation system is established based on the assumption of rigid body of the hub, inextensible tether, and lumped masses of the sub-satellites. Two novel formation deployment controllers are proposed based on tension control and hybrid tension-thrust control strategies, where underactuated sliding mode control and nonsingular terminal sliding mode control method are used, respectively. The adaptive control theory is adopted to estimate the unknown upper bound of the gravitational perturbation caused by the rotation of the system around the hub. It can be proven by the Lyapunov theory that the close-loop systems have bounded and asymptotic stability under these two deployment controllers, respectively. Finally, numerical simulations are conducted to validate the effectiveness and robustness of the proposed controllers.
Applications of Rough Soft to Extensions Semihypergroups Induced by Operators and Corresponding Decision Making Methods
Authors: Khorshid Noreh Rakhsh , Ostadhadi-dehkordi Sohrab 
Published: July 2021

Abstract
In this article, we apply rough soft set to a special algebraic hyperstructure, which obtained of disjoint Γ-semihypergroups, and give the concept of rough soft semihypergroup. We propose the notion of lower
and upper approximations with respect to a special semihypergroup and obtain some properties of them. Moreover, we consider a connection between lower(upper) approximation of a special semihypergroup
and lower(upper) approximation of associated Γ-hypergroupoid. In the last section of this research, we discuss the decision making algorithm of rough soft semihypergroups and we obtain a relation between the decision making algorithm of rough soft semihypergroups and their associated rough soft Γ-hypergroupoid for a special semihypergroup.
Modeling Method of CNC Machine Tools’ Volume Error Considering Abbe Error
Authors: CHEN GUOHUA , Zhang Lin , WANG XIANGJIE , WANG CHAO , XIANG HUA , TONG GUANGQING , ZHAO DIANZHANG 
Published: July 2021

Abstract
Abbe error is an important factor affecting high-precision machine tools, and the traditional modeling method does not consider Abbe error. Aiming at this problem, based on the traditional error model of machine tools and the formation mechanism of Abbe error, this paper establishes a machine tool spatial error model that considers Abbe error. Then combined with a specific machine tool, based on the measurement of 21 geometric errors of the machine tool to obtain relevant error data, through the combination of qualitative and quantitative accuracy evaluation methods, two models of traditional error model and error model considering Abbe error are analyzed. The accuracy of the machine tool is compared, and the comparison of the compensation effects of the two error models after compensation is also analyzed. The example verification shows that the machine tool spatial error model considering Abbe error is effective and feasible, and the compensation effect is better. It provides an important modeling method for improving the machining accuracy of precision machine tools.
Biomechanical Evaluation of a New Anatomic Locking Buttress Plate for Posteromedial Tibial Plateau Fixation.
Authors: Lin Kai Cheng , Tarng Yih Wen , Wei Hung-Wen , Lin Kun-Jhih 
Published: July 2021

Abstract
Background: Dealing with high-energy fractures of the tibial plateau remains a challenge despite advances in implants, surgical approaches, and imaging methods. Posterior buttress plate is most commonly used implant but the fixation stability is still a challenge. Recently, a newly designed tibial locking plate was introduced that aims to provide better fixation strength for tibial plateau split fracture. This study compared the biomechanical strength of three different posteromedial tibial plateau split fracture fixation methods. Methods: The tibial plateau fractures were simulated using a human tibiae model. Each fracture model was virtually implanted with one of the three following constructs, proximal medial tibial plate (PMT), proximal posterior medial tibial plate (PPMT), and posterior T-shaped buttress plate (TBP). Posteromedial fragment vertical subsidence was measured under 2000 N joint contact force. The maximum Equivalent stress on the bone plate and bone screw and the construct stiffness were determined.Results: The proximal medial tibial plate (PMT) allowed the least posteromedial fragment subsidence and produced higher construct stiffness than each of the other two constructs. However, the proximal posterior medial tibial plate (PPMT) showed higher stiffness than the T-shaped buttress plate (TBP). The maximum Equivalent stress was the smallest for the proximal medial tibial plate (PMT).Conclusion: This study showed that the proximal medial tibial locking plate or proximal posterior medial tibial locking plate were biomechanically more stable fixation methods for posteromedial split tibial plateau fractures.
Vertical Graphene Arrays for AC Line-Filtering Capacitors
Authors: Zhang Jin , Xu Shichen , Wen Yeye , Chen Zhuo , Ji Nannan , Wu Mingmao , Zou Zhigang , Qu Liangti 
Published: July 2021

Abstract
High-frequency responsive electrochemical capacitor (EC), which can convert alternating current (AC) in the circuit to direct current (DC), is an ideal filtering capacitor with lightweight superiority to replace the bulky aluminum electrolytic capacitor (AEC). However, current electrodes are difficult to achieve high energy density and high-frequency response properties simultaneously, primarily due to the electrode structure dilemmas of maximizing the electrode area or accelerating the ion transport. Herein, strictly vertical graphene arrays (SVGAs) directly prepared by electric-field-assisted plasma enhanced chemical vapor deposition have been successfully designed as the main electrode material of ECs to ensure the ions rapidly adsorb/desorb within the richly available surface spaces. The SVGAs exhibit an excellent specific areal capacitance of 1.72 mF‧cm− 2 at Φ120 = 80.6° even after 500,000 cycles in the aqueous ECs, which is far better than that of most quasi-vertical electrodes and carbon-related materials. Impressively, the output voltage could also be improved to 2.5 V when using the organic electrolyte, and an ultra-high energy density of 4.75 mF‧V2‧cm− 2 at Φ120 = 80.6° can also be handily achieved. Moreover, both aqueous and organic ECs-SVGAs can well smooth arbitrary AC waveforms into DC signals, indicating that ECs-SVGAs have colossal potentials to replace outmoded AECs.
A Rare Case of Aggressive Systemic Mastocytosis Involving the Gastrointestinal System and Review of Literature
Authors: Wang Jianjun , Zheng Zhong , Niu Feng-nan , Wang Ting , Zhang Biao , Fan Xiang-shan , Yu Hui-ping 
Published: July 2021

Abstract
Background Systemic mastocytosis is a rare disease and most patients have pigmented urticarial skin lesions. It can be easily missed and misdiagnosed in small biopsies, especially in those patients with nonspecific clinical complaints or untypical skin lesions. Case presentation We report a case of 69-year-old man who have presented with 2-year of diarrhea, progressive weight loss of 20kg and abdominal distention for 3 months. Ultrasound and abdominal CT scan showed massive effusions in abdominal and pelvic cavity. Colonoscopy was performed and showed intensive mucosal proliferations forming polypoid appearances. Microscopically, monotonously uniform, small, round tumor cells with slightly rich cytoplasm were concentrated between the residual glands in the colonoscopic biopsy. The tumor cells showed positive expression of CD117 and S-100, and low Ki-67 proliferation index of 2%, while Trypsin, CK, CD68, CD1a, Langerin, Syn, CgA, CD56, SATB2, CD20, CD3, α-inhibin and SMA were all negative. KIT D816V mutation was detected as well. Liver biopsy showed that CD117 positive cells were more than 15 cells in aggregates around the hepatic portal area and less than 15% mast cells were found in bone marrow smears. No multiple or diffuse pattern of mast cells infiltration was seen by repeated skin biopsies of skin lesions. With all these considered, the diagnosis of aggressive systemic mastocytosis was made. Conclusions The diagnosis of aggressive systemic mastocytosis is challenging for untypical clinical manifestations and subtle or inconspicuous lesions, especially in endoscopic biopsies, which requires awareness and a close teamwork of pathologists and clinicians.
Effect of Lead on the Development and Nutrient Accumulation Ability of Musca Domestica
Authors: Wang Wanqiang , Tang Wenzheng , Zhang Jie , Wen Yiting , Wang Xiaoping , Zhu Fen 
Published: July 2021

Abstract
Lead pollution poses a threat to the survival of animals, plants and humans. Environmental pollution conversion with insects is a new type of environmental management. Housefly (Musca domestica) may be an efficient, economical, and safe choice for lead pollution control. However, this needs to make sure that houseflies are strongly resistant to lead contamination. In this study, housefly larvae, pupae and adult growth parameters and adult fecundity under lead treatment were counted, and changes in digestive enzyme activities and gene expression levels were detected to comprehensively analyze the tolerance of housefly to lead exposure. As a result, lead accumulated in the larvae under 5000 mg/kg lead treatment and caused a decrease in the survival rate of the larvae, extension of the developmental duration, decrease of the body weight, and dynamic changes in oviposition dynamics. The total sugar and total protein content of the larvae decreased, while the amylase and trypsin activities decreased, the total lipid content increased, and the lipase activity increased, the expression of all digestive enzyme genes changed. 20 mg/kg lead treatment has no significant effect on the growth and development of housefly. In conclusion, lead exposure mainly affected the growth rate of larvae and adult fecundity by affecting the efficiency of housefly nutrient accumulation.
Promising Diagnostic Accuracy of Plasma GFAP and NfL Within The AD Continuum
Authors: Parvizi Tandis , Koenig Theresa , Wurm Raphael , Silvaieh Sara , Altmann Patrick , Klotz Sigrid , Rommer Paulus , Furtner Julia , Regelsberger Guenther , Lehrner Johann , Traub-Weidinger Tatjana , Gelpi-Mantius Ellen , Stögmann Elisabeth 
Published: July 2021

Abstract
Background: Blood-based biomarkers may add a great benefit in detecting the earliest neuropathological changes in patients with Alzheimer’s disease (AD). We examined the utility of neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP) in plasma and cerebrospinal fluid (CSF) regarding clinical diagnosis and amyloid positivity in an outpatient memory clinic - based cohort. Methods: In this retrospective analysis, we included a total of 185 patients, 141 patients along clinical the AD continuum, i.e. subjective cognitive decline (SCD, n=18), mild cognitive impairment (MCI, n=63), AD (n=60) and 44 age-matched healthy controls (HC). CSF and plasma concentrations of NfL and GFAP were measured with single molecule array (SIMOAâ) technology using the Neurology 2-Plex B kit from Quanterix. Amyloid-PET was performed in 75 patients and graded as amyloid positive and negative by visual rating. To assess the discriminatory potential of different biomarkers, age- and sex-adjusted receiver operating characteristic (ROC) curves were calculated and the area under the curve (AUC) of each model was compared using DeLong’s test for correlated AUC curves.Results: We constructed a panel combining plasma NfL and GFAP with known AD risk factors (age+sex+APOE4+GFAP+NfL panel). Using this panel, AUC was 91.6% for HC vs. AD, 81.7% for HC vs. MCI, 85% for SCD vs. AD, 81.3% for SCD vs. MCI, 77.7% for HC vs. SCD and 72.3% for MCI vs. AD. In terms of predicting amyloid PET status, we computed an AUC of 88.4%. Conclusion: The combination of plasma GFAP and NfL with well-established risk factors could contribute crucially to the identification of patients at risk, and thereby facilitate inclusion of patients in clinical trials for disease modifying therapies.
Differences in Genetics and Microenvironment of Lung Adenocarcinoma Patients with or without TP53 Mutation
Authors: Zeng Dejun , Hu Zhengyang , Yi Yanjun , Valeria Besskaya , Shan Guangyao , Chen Zhencong , Zhan Cheng , Lin Miao , Lin Zongwu , Wang Qun 
Published: July 2021

Abstract
Background: Differences in genetics and microenvironment of lung adenocarcinoma (LUAD) patients with or without TP53 mutation were analyzed to illustrate the role of TP53 mutation within the carcinogenesis of LUAD, which will provide new concepts for the treatment of LUAD.Methods: In this study, we used genetics and clinical info from the TCGA database, including somatic mutations data, RNA-seq, miRNA-seq, and clinical data. More than one bioinformatics tools were used to analyze the unique genomic pattern of TP53-related LUAD.Results: According to TP53 gene mutation status, we divided the LUAD patients into two groups, including 265 in the mutant group (MU) and 295 in the wild-type group (WT). 787 significant somatic mutations were detected between the groups, includingmutations in titin (TTN), type 2 ryanodine receptor (RYR2) and CUB and Sushi multiple domains 3(CSMD3), which were up-regulated in the MU. However, no significant survival difference was observed. At the RNA level, we obtained 923 significantly differentially expressed genes; in the MU, α-defensin 5(DEFA5),pregnancy-specific glycoprotein 5(PSG5) and neuropeptide Y(NPY) were the most up-regulated genes, glucose-6-phosphatase (G6PC), alpha-fetoprotein (AFP) and carry gametocidal (GC) were the most down-regulated genes. GSVA analysis revealed 30 significant pathways. Compared with the WT, the expression of 12 pathways in the mutant group was up-regulated, most of which pointed to cell division. There were significant differences in tumor immune infiltrating cells, such as Macrophages M1, T cells CD4 memory activated, Mast cells resting, and Dendritic cells resting. In terms of immune genes, a total of 35 immune-related genes were screened, of which VGF (VGF nerve growth factor inducible) and PGC (peroxisome proliferator-activated receptor gamma coactivator) were the most significant up-regulated and down-regulated genes, respectively. Research on the expression pattern of immunomodulators found that 9 immune checkpoint molecules and 6 immune costimulatory molecules were considerably wholly different between the two groups.Conclusion: Taking the mutant group as a reference, LUAD patients in the mutant group had significant differences in somatic mutations, mRNA-seq, miRNA-seq, immune infiltration, and immunomodulators, indicating that TP53 mutation plays a crucial role in the occurrence and development of LUAD.
FDI and Health Expenditure Dynamics in ASEAN
Authors: Verma Rakhi 
Published: July 2021

Abstract
Background: Given ASEAN’s steadily increasing levels of foreign direct investment over time, it is imperative to understand how FDI can assist to improve healthcare capacity level which will help the country stay on course to achieve target 3 of the Sustainable Development Goals (SDG) of achieving equal health by 2030. Health is important for any country’s economic development and has been a major goal under United Nations assigned Sustainable Development Goals. Health expenditure is a necessity good at the state level. Studies suggest although globalization is an important engine of growth, role of globalization in health has been rarely studied. Some of the traditional benefits include technology transfer and human skills. Its impact on health been under-investigated. In this paper we aim to understand the dynamics between FDI and health expenditure in ASEAN. Three models, ordinary least square, Quantile and two stage least square regression using Instrumental variables were employed to test the relationship between FDI and healthcare expenditure. Instrumental variables include market size, population health and labor productivity.Results: The correlation index showed that except labor productivity, all variables were strongly correlated. The OLS and quantile regression confirmed that the market size, population health and labor productivity are significant for health expenditure for ASEAN. In the 2SLS instrumental approach, the study finds that there is a strong causal relation between FDI and health expenditure.Conclusion: Our results confirms that FDI is an important factor of human capital and influences the health expenditure capacity of a country in a short and long run. We advocate that the potential of FDI in building nation’s healthcare expenditure capacity is very critical and should be included in policy making. This is among very rare studies which has tested the role of FDI on health expenditure.