bluuebunny/crossref_metadata_2025_split huggingface dataset:
An ultra-wide scanner for large-area high-speed atomic force microscopy with megapixel resolution
Authors: Marchesi Arin , Umeda Kenichi , Komekawa Takumi , Matsubara Takeru , Flechsig Holger , Ando Toshio , Watanabe Shinji , Kodera Noriyuki , Franz Clemens M. 
Published: June 2021

AbstractHigh-speed atomic force microscopy (HS-AFM) is a powerful tool for visualizing the dynamics of individual biomolecules. However, in single-molecule HS-AFM imaging applications, x,y-scanner ranges are typically restricted to a few hundred nanometers, preventing overview observation of larger molecular assemblies, such as 2-dimensional protein crystal growth or fibrillar aggregation. Previous advances in scanner design using mechanical amplification of the piezo-driven x,y-positioning system have extended the size of HS-AFM image frames to several tens of micrometer, but these large scanners may suffer from mechanical instabilities at high scan speeds and only record images with limited pixel numbers and comparatively low lateral resolutions (> 20–100 nm/pixel), complicating single-molecule analysis. Thus, AFM systems able to image large sample areas at high speeds and with nanometer resolution have still been missing. Here, we describe a HS-AFM sample-scanner system able to record large topographic images (≤ 36 × 36 µm2) containing up to 16 megapixels, providing molecular resolution throughout the image frame. Despite its large size, the flexure-based scanner features a high resonance frequency (> 2 kHz) and delivers stable operation even at high scans speeds of up to 7.2 mm/s, minimizing the time required for recording megapixel scans. We furthermore demonstrate that operating this high-speed scanner in time-lapse mode can simultaneously identify areas of spontaneous 2-dimensional Annexin A5 crystal growth, resolve the angular orientation of large crystalline domains, and even detect rare crystal lattice defects, all without changing scan frame size or resolution. Dynamic processes first identified from overview scans can then be further imaged at increased frame rates in reduced scan areas after switching to conventional HS-AFM scanning. The added ability to collect large-area, high-resolution images of complex samples within biological-relevant time frames extends the capabilities of HS-AFM from single-molecule imaging to the study of large dynamic molecular arrays. Moreover, large-area HS-AFM scanning can generate detailed structural data sets from a single scan, aiding the quantitative analysis of structurally heterogenous samples, including cellular surfaces.
Miniaturized tri-notched wideband bandpass filter with ultrawide upper stopband suppression
Authors: Wang Chen-Hao , Shi Xiao-Min 
Published: June 2021

AbstractStepped impedance resonator (SIR) and its derivative resonators are widely used in the design of microwave filters. However, many spurious modes will be introduced into the stopband, resulting in lower upper stopband suppression and performance degradation. Based on the principle of slit line, a method to enhance the upper stopband suppression is proposed and verified by a miniaturized tri-notched wideband bandpass filter based on stub loaded ring resonator (SLRR) and shorted-stub loaded SIR (SSLSIR). The wideband is formed by coupling SLRR and interdigital lines, which has a rectangular DGS on the back of the substrate. Three notched bands with controllable positions in the passband can be produced by inserting two different SSLSIRs inside and outside the SLRR. Four slit lines are loaded on the low impedance stub of SSLSIR to adjust the high-order modes close to the transmission zeros (TZs). The operating frequency of the filter is 2.2–7.6 GHz, and the three notched bands are located at 2.97 GHz, 5.75 GHz and 6.46 GHz, respectively. The measurement results show that the − 20 dB enhanced upper stopband of the filter can reach 32 GHz, which proves that the filter has the characteristic of ultrawide upper stopband suppression while keeping the miniaturization.
Monitoring abundance of aggregated animals (Florida manatees) using an unmanned aerial system (UAS)
Authors: Edwards Holly H. , Hostetler Jeffrey A. , Stith Bradley M. , Martin Julien 
Published: June 2021

AbstractImperfect detection is an important problem when counting wildlife, but new technologies such as unmanned aerial systems (UAS) can help overcome this obstacle. We used data collected by a UAS and a Bayesian closed capture-mark-recapture model to estimate abundance and distribution while accounting for imperfect detection of aggregated Florida manatees (Trichechus manatus latirostris) at thermal refuges to assess use of current and new warmwater sources in winter. Our UAS hovered for 10 min and recorded 4 K video over sites in Collier County, FL. Open-source software was used to create recapture histories for 10- and 6-min time periods. Mean estimates of probability of detection for 1-min intervals at each canal varied by survey and ranged between 0.05 and 0.92. Overall, detection probability for sites varied between 0.62 and 1.00 across surveys and length of video (6 and 10 min). Abundance varied by survey and location, and estimates indicated that distribution changed over time, with use of the novel source of warmwater increasing over time. The highest cumulative estimate occurred in the coldest winter, 2018 (N = 158, CI 141–190). Methods here reduced survey costs, increased safety and obtained rigorous abundance estimates at aggregation sites previously too difficult to monitor.
Effect of nonionic and amphoteric surfactants on salivary pellicles reconstituted in vitro
Authors: Boyd Hannah , Gonzalez-Martinez Juan F. , Welbourn Rebecca J. L. , Ma Kun , Li Peixun , Gutfreund Philipp , Klechikov Alexey , Arnebrant Thomas , Barker Robert , Sotres Javier 
Published: June 2021

AbstractSurfactants are important components of oral care products. Sodium dodecyl sulfate (SDS) is the most common because of its foaming properties, taste and low cost. However, the use of ionic surfactants, especially SDS, is related to several oral mucosa conditions. Thus, there is a high interest in using non-ionic and amphoteric surfactants as they are less irritant. To better understand the performance of these surfactants in oral care products, we investigated their interaction with salivary pellicles i.e., the proteinaceous films that cover surfaces exposed to saliva. Specifically, we focused on pentaethylene glycol monododecyl ether (C12E5) and cocamidopropyl betaine (CAPB) as model nonionic and amphoteric surfactants respectively, and investigated their interaction with reconstituted salivary pellicles with various surface techniques: Quartz Crystal Microbalance with Dissipation, Ellipsometry, Force Spectroscopy and Neutron Reflectometry. Both C12E5 and CAPB were gentler on pellicles than SDS, removing a lower amount. However, their interaction with pellicles differed. Our work indicates that CAPB would mainly interact with the mucin components of pellicles, leading to collapse and dehydration. In contrast, exposure to C12E5 had a minimal effect on the pellicles, mainly resulting in the replacement/solubilisation of some of the components anchoring pellicles to their substrate.
Factors affecting hallucinations in patients with delirium
Authors: Tachibana Masako , Inada Toshiya , Ichida Masaru , Ozaki Norio 
Published: June 2021

AbstractDelirium develops through a multifactorial process and include multiple subtypes with different pathological factors. To refine the treatment and care for delirium, a more detailed examination of these subtypes is needed. Therefore, this study aimed to explore the factors affecting delirium in cases in which hallucinations are conspicuous. In total, 602 delirium cases referred to the psychiatry department at a general hospital between May 2015 and August 2020 were enrolled. The Delirium Rating Scale-revised-98 was used to assess perceptual disturbances and hallucinations in patients with delirium. Multiple regression analysis was applied to determine whether individual factors were associated with the hallucinations. A total of 156 patients with delirium (25.9%) experienced hallucinations, with visual hallucinations being the most common subtype. Alcohol drinking (p < 0.0005), benzodiazepine withdrawal (p = 0.004), and the use of angiotensin II receptor blockers (p = 0.007) or dopamine receptor agonists (p = 0.014) were found to be significantly associated with hallucinations in patients with delirium. The four factors detected in this study could all be reversible contributing factors derived from the use of or withdrawal from exogenous substances.
Deficit or Difference? Effects of Altered Auditory Feedback on Speech Fluency and Kinematic Variability in Adults Who Stutter
Authors: Chon HeeCheong , Jackson Eric S. , Kraft Shelly Jo , Ambrose Nicoline G. , Loucks Torrey M. 
Published: June 2021

Purpose
The purpose of this study was to test whether adults who stutter (AWS) display a different range of sensitivity to delayed auditory feedback (DAF). Two experiments were conducted to assess the fluency of AWS under long-latency DAF and to test the effect of short-latency DAF on speech kinematic variability in AWS.

Method
In Experiment 1, 15 AWS performed a conversational speaking task under nonaltered auditory feedback and 250-ms DAF. The rates of stuttering-like disfluencies, other disfluencies, and speech errors and articulation rate were compared. In Experiment 2, 13 AWS and 15 adults who do not stutter (AWNS) read three utterances under four auditory feedback conditions: nonaltered auditory feedback, amplified auditory feedback, 25-ms DAF, and 50-ms DAF. Across-utterance kinematic variability (spatiotemporal index) and within-utterance variability (percent determinism and stability) were compared between groups.

Results
In Experiment 1, under 250-ms DAF, the rate of stuttering-like disfluencies and speech errors increased significantly, while articulation rate decreased significantly in AWS. In Experiment 2, AWS exhibited higher kinematic variability than AWNS across the feedback conditions. Under 25-ms DAF, the spatiotemporal index of AWS decreased significantly compared to the other feedback conditions. AWS showed lower overall percent determinism than AWNS, but their percent determinism increased under 50-ms DAF to approximate that of AWNS.

Conclusions
Auditory feedback manipulations can alter speech fluency and kinematic variability in AWS. Longer latency auditory feedback delays induce speech disruptions, while subtle auditory feedback manipulations potentially benefit speech motor control. Both AWS and AWNS are susceptible to auditory feedback during speech production, but AWS appear to exhibit a distinct continuum of sensitivity.
Calibration of mid- to far-infrared spectral lines in galaxies
Authors: Mordini Sabrina , Spinoglio Luigi , Fernández-Ontiveros Juan Antonio 
Published: June 2021

Context. Mid- to far-infrared (IR) lines are suitable in the study of dust-obscured regions in galaxies because dust extinction strongly decreases with wavelength, and therefore IR spectroscopy allows us to explore the most hidden regions of galaxies, where heavily obscured star formation as well as accretion onto supermassive black holes at the nuclei of galaxies occur. This is mostly important for the so-called cosmic noon (i.e. at redshifts of 1 < z < 3), at which point most of the baryonic mass in galaxies has been assembled.
Aims. Our goal is to provide reliable calibrations of the mid- to far-IR ionic fine-structure lines, the brightest H2 pure rotational lines, and the polycyclic aromatic hydrocarbon (PAH) features, which we used to analyse current and future observations in the mm-submm range from the ground, as well as mid-IR spectroscopy from the upcoming James Webb Space Telescope.
Methods. We used three samples of galaxies observed in the local Universe: star-forming galaxies (SFGs, 196), active galactic nuclei (AGN; 90−150 for various observables), and low-metallicity dwarf galaxies (40). For each population, we derive different calibrations of the observed line luminosities versus the total IR luminosities.
Results. Through the resulting calibrations, we derive spectroscopic measurements of the star formation rate (SFR) and of the black hole accretion rate (BHAR) in galaxies using mid- and far-IR fine-structure lines, H2 pure rotational lines and PAH features. In particular, we derive robust star formation tracers based on the following: the [CII]158 μm line; the sum of the two far-IR oxygen lines, the [OI]63 μm line, and the [OIII]88 μm line; a combination of the neon and sulfur mid-IR lines; the bright PAH features at 6.2 and 11.3 μm; as well as – for the first time – the H2 rotational lines at 9.7, 12.3, and 17 μm. We propose the [CII]158 μm line, the combination of the two neon lines ([NeII]12.8 μm and [NeIII]15.5 μm), and, for solar-like metallicity galaxies that may harbour an AGN, the PAH 11.3 μm feature as the best SFR tracers. On the other hand, a reliable measure of the BHAR can be obtained using the [OIV]25.9 μm and the [NeV]14.3 and 24.3 μm lines. For the most commonly observed fine-structure lines in the far-IR, we compare our calibration with the existing ALMA observations of high-redshift galaxies. We find an overall good agreement for the [CII]158 μm line for both AGN and SFGs, while the [OIII]88 μm line in high-z galaxies is in better agreement with the low-metallicity local galaxies (dwarf galaxy sample) than with the SFGs, suggesting that high-z galaxies might have strong radiation fields due to low metal abundances, as expected.
Perioperatives Management – Aufgaben und Bedeutung von speziell geschulten Pflegekräften
Authors: Koch Franziska , Green Martina , Dietrich Melanie , Moikow Lutz , Pontau Frauke , Ulmer Stefanie , Dietrich Niels , Ritz Jörg-Peter 
Published: June 2021

ZusammenfassungModerne perioperative Behandlungskonzepte legen großen Wert auf eine aktive Rolle der Patienten. Um dies zu ermöglichen, ist eine intensive Patientenbegleitung notwendig. Aus dieser Notwendigkeit heraus hat sich die Position der ERAS®-Nurse entwickelt. Sie ist der primäre Ansprechpartner für den Patienten vor, während und nach der Operation. Die konzeptionelle Erstellung und fortwährende Weiterentwicklung eines ERAS-Konzeptes mag vor allem ärztliche Tätigkeit sein, die tagtägliche Arbeit am Patienten und die Füllung des Konzeptes mit Leben wird jedoch vor allem durch eine ERAS-Nurse bewerkstelligt. Ihre Hauptaufgaben liegen in der präoperativen Patientenaufklärung, der täglichen Visitation der Patienten während des stationären Aufenthaltes, dem Ausfüllen der Dokumentation, der fortwährenden Kommunikation mit Pflegekräften und dem Monitoring der Einhaltung der ERAS-Vorgaben aller beteiligten Teammitglieder. Entsprechend wichtig ist es, die Position der
ERAS-Nurse zu schaffen und sie als wertiges Mitglied ins Team zu integrieren. Im folgenden Artikel, der auf unseren Erfahrungen als zertifiziertes ERAS-Zentrum beruht, wird das Berufsbild umfänglich vorgestellt, einschl. Aufgaben, Bedeutung und Lösungsvorschlägen für gängige Probleme.
Multi-expert annotation of Crohn’s disease images of the small bowel for automatic detection using a convolutional recurrent attention neural network
Authors: de Maissin Astrid , Vallée Remi , Flamant Mathurin , Fondain-Bossiere Marie , Berre Catherine Le , Coutrot Antoine , Normand Nicolas , Mouchère Harold , Coudol Sandrine , Trang Caroline , Bourreille Arnaud 
Published: June 2021

Abstract
Background and study aims Computer-aided diagnostic tools using deep neural networks are efficient for detection of lesions in endoscopy but require a huge number of images. The impact of the quality of annotation has not been tested yet. Here we describe a multi-expert annotated dataset of images extracted from capsules from Crohn’s disease patients and the impact of the quality of annotations on the accuracy of a recurrent attention neural network.
Methods Images of capsule were annotated by a reader first and then reviewed by three experts in inflammatory bowel disease. Concordance analysis between experts was evaluated by Fleiss’ kappa and all the discordant images were, again, read by all the endoscopists to obtain a consensus annotation. A recurrent attention neural network developed for the study was tested before and after the consensus annotation. Available neural networks (ResNet and VGGNet) were also tested under the same conditions.
Results The final dataset included 3498 images with 2124 non-pathological (60.7 %), 1360 pathological (38.9 %), and 14 (0.4 %) inconclusive. Agreement of the experts was good for distinguishing pathological and non-pathological images with a kappa of 0.79 (P < 0.0001). The accuracy of our classifier and the available neural networks increased after the consensus annotation with a precision of 93.7 %, sensitivity of 93 %, and specificity of 95 %.
Conclusions The accuracy of the neural network increased with improved annotations, suggesting that the number of images needed for the development of these systems could be diminished using a well-designed dataset.
New Therapeutic Strategies in Advanced Nonoperable or Metastatic HER2-positive Breast Cancer
Authors: Lüftner Diana , Peipp Matthias 
Published: June 2021

AbstractDespite therapeutic gains in the treatment of HER2-positive (HER2: human epidermal growth factor receptor 2) advanced/metastatic breast cancer, there remains an urgent need for more effective treatment options. At present, there is no definitive approved standard therapy beyond second-line treatment. One of the major challenges is overcoming treatment resistance. Depending on the underlying resistance mechanism, different strategies are being pursued for new innovative treatment concepts in HER2-positive breast cancer. Specifically designed antibodies for targeted therapy are one important focus to successfully meet these challenges. Trastuzumab deruxtecan (T-DXd, DS-8201a), an optimised antibody drug conjugate (ADC) is in clinical trials, showing promising outcomes in patients with advanced, nonoperable or metastatic HER2-positive breast cancer who had already undergone intensive prior treatment. Based on this data, T-DXd has already been approved in the US and Japan for HER2-positive advanced nonoperable and metastatic breast cancer – in the US after at least two prior anti-HER2 targeted treatment lines and in Japan after prior chemotherapy. T-DXd represents successful “antibody engineering”. Since the beginning of the year, T-DXd has also been approved in Europe as monotherapy for inoperable or metastatic HER2-positive breast cancer in patients who are pretreated with at least two anti-HER2 directed therapies. This paper presents strategies for improving treatment options in advanced nonoperable and metastatic HER2-positive breast cancer, with the development of T-DXd as an example.
Reliability of the Endoscopic Ultrasound Ulcerative Colitis (EUS-UC) score for assessment of inflammation in patients with ulcerative colitis
Authors: Yan Brian M. , Sey Michael S.L. , Belletrutti Paul , Brahm Gary , Guizzetti Leonardo , Jairath Vipul 
Published: June 2021

Abstract
Background and study aims Endoscopic ultrasound (EUS) may be a useful modality for disease assessment and risk stratification in ulcerative colitis. We assessed the reliability of a newly developed EUS index of inflammation called the EUS-Ulcerative Colitis (EUS-UC) score.
Patients and methods The EUS-UC score components include total wall thickness, hyperemia, and depth of inflammation (DOI). Three blinded expert endosonographers assessed EUS videos of 58 patients with UC in triplicate. Intra- and inter-rater reliability of the hyperemia and DOI component scores were estimated using intra-class correlation coefficients (ICCs). Total wall thickness reliability estimates could not be assessed in this study. The ICCs were compared to the original indices from which they were derived.
Results For hyperemia, the inter-class ICC was “moderate” at 0.556 (95 % CI = 0.434–0.651) and the intra class ICC was “almost perfect” at 0.884 (95 % CI = 0.835–0.920). The newly defined hyperemia score performed better than the original index from which is was derived. The DOI inter-class ICC was “fair” at 0.335 (95 % CI = 0.201–0.464), and the intra-class ICC was “substantial” at 0.732 (95 % CI = 0.642–0.802). The DOI reliability estimates were similar to the original index from which it was derived.
Conclusions The hyperemia component of the EUS-UC score performed significantly better than the original index from which it was derived, but the reliability of the DOI component was suboptimal. Intra-class correlation was excellent for both components. The EUS-UC score is a promising instrument for assessment of UC and further validation is required.
Patterns and Trends of Herbal Medicine Use among Patients with Gynecologic Cancer
Authors: Theuser Anna-Katharin , Hack Carolin C. , Fasching Peter A. , Antoniadis Sophia , Grasruck Katharina , Wasner Sonja , Knoll Stefanie , Sievers Hartwig , Beckmann Matthias W. , Thiel Falk C. 
Published: June 2021

Abstract
Background More and more information about complementary and integrative medicine is becoming available, especially among cancer patients. However, little is known about the use of herbal medicine by patients with gynecologic cancers. This study aimed to assess the use of herbal products by gynecologic cancer patients compared with healthy controls.
Methods This cross-sectional study was conducted at the Department for Gynecology and Obstetrics of Erlangen University Hospital and included 201 patients with gynecologic cancer and 212 healthy controls. Use of herbal medicines was evaluated using a standardized questionnaire. Medical information on cancer patients was collected from hospital records. Group comparisons were done using a logistic regression model. Risk ratios were assessed using a Poisson regression model.
Results Gynecologic cancer patients used herbal medicine significantly less often than healthy persons. 69% of gynecologic cancer patients and 81% of healthy participants reported using herbal products. 40% of cancer patients and 56% of healthy persons reported using plants for medicinal purposes. Motives of cancer patients for using herbal medicine included treatment of cancer-related symptoms. The major source of information for both groups was family and friends.
Conclusions Although herbal medicine was used less by patients with gynecologic cancer, herbal products were used by both cancer patients and healthy individuals. To provide cancer patients with optimal therapy, oncologists should be informed about the herbal products used by their patients as this will allow them to take their patientsʼ self-medication with herbal medicine into account. Counseling by oncologists on the use of herbal medicine should be encouraged.