bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radio-echo sounding and waveform modeling reveal abundant marine ice in former rifts and basal crevasses within Crary Ice Rise, Antarctica
Authors: Hillebrand Trevor R. , Conway Howard , Koutnik Michelle , Martín Carlos , Paden John , Winberry J. Paul 
Published: March 2021

AbstractCrary Ice Rise formed after the Ross Ice Shelf re-grounded ~1 kyr BP. We present new ice-penetrating radar data from two systems operating at center frequencies of 7 and 750 MHz that confirm the ice rise is composed of a former ice shelf buried by subsequent accumulation. Stacks of englacial diffraction hyperbolas are present almost everywhere across the central ice rise and extend up to ~350 m above the bed. In many cases, bed reflections beneath the diffraction hyperbolas are obscured for distances up to 1 km. Waveform modeling indicates that the diffraction hyperbolas are likely caused by marine ice deposits in former basal crevasses and rifts. The in-filling of rifts and basal crevasses may have strengthened the connection between the ice rise and the surrounding ice shelf, which could have influenced local and regional ice dynamics. Three internal reflection horizons mark the upper limit of disturbed ice and diffraction hyperbolas in different sections of the ice rise, indicating at least three stages of flow stabilization across the ice rise. A surface lineation visible in MODIS imagery corresponds spatially to deepening and strong deformation of these layers, consistent with the characteristics of former grounding lines observed elsewhere in Antarctica.
Preliminary Factor Structure of the Parental Attitudes of Gender Expansiveness Scale for Parents (PAGES-P)
Authors: Reguitti Vanessa , Poquiz Jonathan L. , Jackson Kathryn , Coyne Claire A. , Hidalgo Marco A. , Forbes Catherine , Chen Diane 
Published: March 2021

Objective: Parental acceptance and support are associated with positive psychosocial outcomes among transgender and gender expansive (TGE) adolescents. Understanding the degree of parental acceptance and support of gender identity and expression is an important component of gender affirmative pediatric assessment and can inform intervention. Although there are reliable measures assessing general family support, there are no existing parent self-report measures assessing acceptance and support of their gender expansive children. The present study examines the factor structure of the Parental Attitudes of Gender Expansiveness Scale for Parents (PAGES-P). Methods: Participants included 739 parents who completed the PAGES-P as standard of care during their child’s gender health clinic visit within a children’s hospital in the midwestern United States. A principal component analysis was used to identify subscales reflected in the PAGES-P. Results: The principal component analysis yielded 4 subscales reflecting the following domains: (a) support and affirmation, (b) guilt and loss, (c) gender concealment, and (d) pride. Conclusions: This study provides preliminary evidence of the factor structure of the PAGES-P. The resulting subscales lend insight into the thoughts and behaviors of parents of TGE youth and can inform clinical practice to facilitate parental support and promote overall well-being in TGE youth. Implications for Impact Statement The Parental Attitudes of Gender Expansiveness Scale for Parents includes four subscales: (a) support and affirmation, (b) guilt and loss, (c) gender concealment, and (d) pride. This questionnaire can be used clinically to identify parents who may benefit from intervention to better support their transgender and gender expansive children.
Clinical and prognostic significance of small paroxysmal nocturnal hemoglobinuria clones in myelodysplastic syndrome and aplastic anemia
Authors: Fattizzo Bruno , Ireland Robin , Dunlop Alan , Yallop Deborah , Kassam Shireen , Large Joanna , Gandhi Shreyans , Muus Petra , Manogaran Charles , Sanchez Katy , Consonni Dario , Barcellini Wilma , Mufti Ghulam J. , Marsh Judith C. W. , Kulasekararaj Austin G. 
Published: March 2021

AbstractIn this large single-centre study, we report high prevalence (25%) of, small (<10%) and very small (<1%), paroxysmal nocturnal hemoglobinuria (PNH) clones by high-sensitive cytometry among 3085 patients tested. Given PNH association with bone marrow failures, we analyzed 869 myelodysplastic syndromes (MDS) and 531 aplastic anemia (AA) within the cohort. PNH clones were more frequent and larger in AA vs. MDS (p = 0.04). PNH clone, irrespective of size, was a good predictor of response to immunosuppressive therapy (IST) and to stem cell transplant (HSCT) (in MDS: 84% if PNH+ vs. 44.7% if PNH−,p = 0.01 for IST, and 71% if PNH+ vs. 56.6% if PNH− for HSCT; in AA: 78 vs. 50% for IST,p < 0.0001, and 97 vs. 77%,p = 0.01 for HSCT). PNH positivity had a favorable impact on disease progression (0.6% vs. 4.9% IPSS-progression in MDS,p < 0.005; and 2.1 vs. 6.9% progression to MDS in AA,p = 0.01), leukemic evolution (6.8 vs. 12.7%,p = 0.01 in MDS), and overall survival [73% (95% CI 68–77) vs. 51% (48–54),p < 0.0001], with a relative HR for mortality of 2.37 (95% CI 1.8–3.1;p < 0.0001) in PNH negative cases, both in univariate and multivariable analysis. Our data suggest systematic PNH testing in AA/MDS, as it might allow better prediction/prognostication and consequent clinical/laboratory follow-up timing.
Broadband vectorial ultrathin optics with experimental efficiency up to 99% in the visible region via universal approximators
Authors: Getman F. , Makarenko M. , Burguete-Lopez A. , Fratalocchi A. 
Published: March 2021

AbstractIntegrating conventional optics into compact nanostructured surfaces is the goal of flat optics. Despite the enormous progress in this technology, there are still critical challenges for real-world applications due to the limited operational efficiency in the visible region, on average lower than 60%, which originates from absorption losses in wavelength-thick (≈ 500 nm) structures. Another issue is the realization of on-demand optical components for controlling vectorial light at visible frequencies simultaneously in both reflection and transmission and with a predetermined wavefront shape. In this work, we developed an inverse design approach that allows the realization of highly efficient (up to 99%) ultrathin (down to 50 nm thick) optics for vectorial light control with broadband input–output responses in the visible and near-IR regions with a desired wavefront shape. The approach leverages suitably engineered semiconductor nanostructures, which behave as a neural network that can approximate a user-defined input–output function. Near-unity performance results from the ultrathin nature of these surfaces, which reduces absorption losses to near-negligible values. Experimentally, we discuss polarizing beam splitters, comparing their performance with the best results obtained from both direct and inverse design techniques, and new flat-optics components represented by dichroic mirrors and the basic unit of a flat-optics display that creates full colours by using only two subpixels, overcoming the limitations of conventional LCD/OLED technologies that require three subpixels for each composite colour. Our devices can be manufactured with a complementary metal-oxide-semiconductor (CMOS)-compatible process, making them scalable for mass production at low cost.
Laser printed two-dimensional transition metal dichalcogenides
Authors: Abbas Omar Adnan , Lewis Adam Henry , Aspiotis Nikolaos , Huang Chung-Che , Zeimpekis Ioannis , Hewak Daniel W. , Sazio Pier , Mailis Sakellaris 
Published: March 2021

AbstractLaser processing is a highly versatile technique for the post-synthesis treatment and modification of transition metal dichalcogenides (TMDCs). However, to date, TMDCs synthesis typically relies on large area CVD growth and lithographic post-processing for nanodevice fabrication, thus relying heavily on complex, capital intensive, vacuum-based processing environments and fabrication tools. This inflexibility necessarily restricts the development of facile, fast, very low-cost synthesis protocols. Here we show that direct, spatially selective synthesis of 2D-TMDCs devices that exhibit excellent electrical, Raman and photoluminescence properties can be realized using laser printing under ambient conditions with minimal lithographic or thermal overheads. Our simple, elegant process can be scaled via conventional laser printing approaches including spatial light modulation and digital light engines to enable mass production protocols such as roll-to-roll processing.
Limited flexibility in departure timing of migratory passerines at the East-Mediterranean flyway
Authors: Aharon-Rotman Yaara , Perlman Gidon , Kiat Yosef , Raz Tal , Balaban Amir , Iwamura Takuya 
Published: March 2021

AbstractThe rapid pace of current global warming lead to the advancement of spring migration in the majority of long-distance migratory bird species. While data on arrival timing to breeding grounds in Europe is plentiful, information from the African departure sites are scarce. Here we analysed changes in arrival timing at a stopover site in Israel and any links to Enhanced Vegetation Index (EVI) on the species-specific African non-breeding range in three migratory passerines between 2000–2017. Differences in wing length between early and late arriving individuals were also examined as a proxy for migration distance. We found that male redstart, but not females, advanced arrival to stopover site, but interestingly, not as a response to EVI phenology. Blackcap and barred warbler did not shift arrival timing significantly, although the arrival of blackcap was dependent on EVI. Barred warbler from the early arrival phase had longer wings, suggesting different populations. Our study further supports the existence species-specific migration decisions and inter-sexual differences, which may be triggered by both exogenous (local vegetation condition) and endogenous cues. Given rapid rate of changes in environmental conditions at higher latitudes, some migrants may experience difficulty in the race to match global changes to ensure their survival.
No time to rest: seasonal dynamics of non-structural carbohydrates in twigs of three Mediterranean tree species suggest year-round activity
Authors: Davidson Anna M. , Le Sylvia T. , Cooper Katelyn B. , Lange Eden , Zwieniecki M. A. 
Published: March 2021

AbstractPerennial plants in temperate climates evolved short and long-term strategies to store and manage reserves in the form of non-structural carbohydrates (NSC; soluble sugars (SC) and starch (St)). NSC storage allows plants to survive seasonal periods of photosynthetic inactivity (dormancy). To study year-to-year seasonal patterns of trees’ NSC dynamics that control phenology and yields, we established a large scale, multi-year study called the “Carbohydrate Observatory” using a citizen science approach with ~ 590 sites throughout the Central Valley of California. Monthly sampling tracked seasonal trends of starch and sugar levels in both xylem and phloem of twigs in Prunus dulcis, Pistacia vera and Juglans regia. Presented is the initial technical analysis of the first 3 years. With no exception, levels of reserves changed continuously throughout the year suggesting that even during dormancy, the average concentration of NSC, starch and sugars varies seasonally. In general, carbohydrate reserves are highest entering dormancy. During winter, NSCs slowly decrease to depletion during bloom time and remain low during summer until recovery near harvest. Starch is the major reserve compound in the wood of P. dulcis and P. vera while soluble sugars are the major reserves in J. regia. NSC content fluctuates throughout a season and significantly varies between years suggesting intrinsic and climatic effects on trees’ energy reserves.
A two-tiered targeted proteomics approach to identify pre-diagnostic biomarkers of colorectal cancer risk
Authors: Harlid Sophia , Harbs Justin , Myte Robin , Brunius Carl , Gunter Marc J. , Palmqvist Richard , Liu Xijia , Van Guelpen Bethany 
Published: March 2021

AbstractColorectal cancer prognosis is dependent on stage, and measures to improve early detection are urgently needed. Using prospectively collected plasma samples from the population-based Northern Sweden Health and Disease Study, we evaluated protein biomarkers in relation to colorectal cancer risk. Applying a two-tiered approach, we analyzed 160 proteins in matched sequential samples from 58 incident colorectal cancer case–control pairs. Twenty-one proteins selected from both this discovery phase and the literature were then analyzed in a validation set of 450 case–control pairs. Odds ratios were estimated by conditional logistic regression. LASSO regression and ROC analysis were used for multi-marker analyses. In the main validation analysis, no proteins retained statistical significance. However, exploratory subgroup analyses showed associations between FGF-21 and colon cancer risk (multivariable OR per 1 SD: 1.23 95% CI 1.03–1.47) as well as between PPY and rectal cancer risk (multivariable OR per 1 SD: 1.47 95% CI 1.12–1.92). Adding protein markers to basic risk predictive models increased performance modestly. Our results highlight the challenge of developing biomarkers that are effective in the asymptomatic, prediagnostic window of opportunity for early detection of colorectal cancer. Distinguishing between cancer subtypes may improve prediction accuracy. However, single biomarkers or small panels may not be sufficient for effective precision screening.
A signed network perspective on the government formation process in parliamentary democracies
Authors: Fontan Angela , Altafini Claudio 
Published: March 2021

AbstractIn parliamentary democracies, government negotiations talks following a general election can sometimes be a long and laborious process. In order to explain this phenomenon, in this paper we use structural balance theory to represent a multiparty parliament as a signed network, with edge signs representing alliances and rivalries among parties. We show that the notion of frustration, which quantifies the amount of “disorder” encoded in the signed graph, correlates very well with the duration of the government negotiation talks. For the 29 European countries considered in this study, the average correlation between frustration and government negotiation talks ranges between 0.42 and 0.69, depending on what information is included in the edges of the signed network. Dynamical models of collective decision-making over signed networks with varying frustration are proposed to explain this correlation.
Using the Microwell-mesh to culture microtissues in vitro and as a carrier to implant microtissues in vivo into mice
Authors: Monterosso Melissa E. , Futrega Kathryn , Lott William B. , Vela Ian , Williams Elizabeth D. , Doran Michael R. 
Published: March 2021

AbstractProstate cancer (PCa) patient-derived xenografts (PDXs) are commonly propagated by serial transplantation of “pieces” of tumour in mice, but the cellular composition of pieces is not standardised. Herein, we optimised a microwell platform, the Microwell-mesh, to aggregate precise numbers of cells into arrays of microtissues, and then implanted the Microwell-mesh into NOD-scid IL2γ−/− (NSG) mice to study microtissue growth. First, mesh pore size was optimised using microtissues assembled from bone marrow-derived stromal cells, with mesh opening dimensions of 100×100 μm achieving superior microtissue vascularisation relative to mesh with 36×36 μm mesh openings. The optimised Microwell-mesh was used to assemble and implant PCa cell microtissue arrays (hereafter microtissues formed from cancer cells are referred to as microtumours) into mice. PCa cells were enriched from three different PDX lines, LuCaP35, LuCaP141, and BM18. 3D microtumours showed greater in vitro viability than 2D cultures, but neither proliferated. Microtumours were successfully established in mice 81% (57 of 70), 67% (4 of 6), 76% (19 of 25) for LuCaP35, LuCaP141, and BM18 PCa cells, respectively. Microtumour growth was tracked using live animal imaging for size or bioluminescence signal. If augmented with further imaging advances and cell bar coding, this microtumour model could enable greater resolution of PCa PDX drug response, and lead to the more efficient use of animals. The concept of microtissue assembly in the Microwell-mesh, and implantation in vivo may also have utility in implantation of islets, hair follicles or other organ-specific cells that self-assemble into 3D structures, providing an important bridge between in vitro assembly of mini-organs and in vivo implantation.
Ambient noise exposure induces long-term adaptations in adult brainstem neurons
Authors: Siveke Ida , Myoga Mike H. , Grothe Benedikt , Felmy Felix 
Published: March 2021

AbstractTo counterbalance long-term environmental changes, neuronal circuits adapt the processing of sensory information. In the auditory system, ongoing background noise drives long-lasting adaptive mechanism in binaural coincidence detector neurons in the superior olive. However, the compensatory cellular mechanisms of the binaural neurons in the medial superior olive (MSO) to long-term background changes are unexplored. Here we investigated the cellular properties of MSO neurons during long-lasting adaptations induced by moderate omnidirectional noise exposure. After noise exposure, the input resistance of MSO neurons of mature Mongolian gerbils was reduced, likely due to an upregulation of hyperpolarisation-activated cation and low voltage-activated potassium currents. Functionally, the long-lasting adaptations increased the action potential current threshold and facilitated high frequency output generation. Noise exposure accelerated the occurrence of spontaneous postsynaptic currents. Together, our data suggest that cellular adaptations in coincidence detector neurons of the MSO to continuous noise exposure likely increase the sensitivity to differences in sound pressure levels.
Japanese Macaques’ (Macaca fuscata) sensitivity to human gaze and visual perspective in contexts of threat, cooperation, and competition
Authors: Castellano-Navarro Alba , Macanás-Martínez Emilio , Xu Zhihong , Guillén-Salazar Federico , MacIntosh Andrew J. J. , Amici Federica , Albiach-Serrano Anna 
Published: March 2021

AbstractGaze sensitivity allows us to interpret the visual perspective of others, inferring their intentions and attentional states. In order to clarify the evolutionary history of this ability, we assessed the response of free-ranging Japanese macaques (Macaca fuscata) to human gaze in three contexts: threat (Experiment 1), cooperation (Experiment 2), and competition (Experiment 3). Subjects interpreted the direct gaze of an approaching human as a sign of threat, showing a greater flight initiation distance and more threats towards the human in this condition than when the human gazed in another direction. Subjects also adapted their behavior to the attentional cues of a human who gave them food, by for example moving into his visual field. However, the macaques did not seem to take the visual perspective of a human competing with them over food, as they failed to first retrieve the food that was not visible to the human (i.e., located behind an opaque barrier). Our results support the idea that Japanese macaques can respond to a human’s gaze flexibly depending on the context. Moreover, they highlight the importance of studying animal behavior across different species and contexts to better understand the selective pressures that might have led to its evolution.
Sonoporation generates downstream cellular impact after membrane resealing
Authors: Duan Xinxing , Zhou Qian , Wan Jennifer M. F. , Yu Alfred C. H. 
Published: March 2021

AbstractSonoporation via microbubble-mediated ultrasound exposure has shown potential in drug and gene delivery. However, there is a general lack of mechanistic knowledge on sonoporation-induced cellular impact after membrane resealing, and this issue has made it challenging to apply sonoporation efficiently in practice. Here, we present new evidence on how sonoporation, without endangering immediate cell viability, may disrupt downstream cellular hemostasis in ways that are distinguished from the bioeffects observed in other sonicated and unsonoporated cells. Sonoporation was realized on HL-60 leukemia cells by delivering pulsed ultrasound (1 MHz frequency, 0.50 MPa peak negative pressure; 10% duty cycle; 30 s exposure period; 29.1 J/cm2 acoustic energy density) in the presence of lipid-shelled microbubbles (1:1 cell-to-bubble ratio). Results showed that 54.6% of sonoporated cells, despite remaining initially viable, underwent apoptosis or necrosis at 24 h after sonoporation. Anti-proliferation behavior was also observed in sonoporated cells as their subpopulation size was reduced by 43.8% over 24 h. Preceding these cytotoxic events, the percentages of sonoporated cells in different cell cycle phases were found to be altered by 12 h after exposure. As well, for sonoporated cells, their expressions of cytoprotective genes in the heat shock protein-70 (HSP-70) family were upregulated by at least 4.1 fold at 3 h after exposure. Taken altogether, these findings indicate that sonoporated cells attempted to restore homeostasis after membrane resealing, but many of them ultimately failed to recover. Such mechanistic knowledge should be taken into account to devise more efficient sonoporation-mediated therapeutic protocols.
Antibodies to SARS-CoV-2 and risk of past or future sick leave
Authors: Dillner Joakim , Elfström K. Miriam , Blomqvist Jonas , Eklund Carina , Lagheden Camilla , Nordqvist-Kleppe Sara , Hellström Cecilia , Olofsson Jennie , Andersson Eni , Jernbom Falk August , Bergström Sofia , Hultin Emilie , Pin Elisa , Månberg Anna , Nilsson Peter , Hedhammar My , Hober Sophia , Mattsson Johan , Mühr Laila Sara Arroyo , Conneryd Lundgren Kalle 
Published: March 2021

AbstractThe extent that antibodies to SARS-CoV-2 may protect against future virus-associated disease is unknown. We invited all employees (n = 15,300) at work at the Karolinska University Hospital, Stockholm, Sweden to participate in a study examining SARS-Cov-2 antibodies in relation to registered sick leave. For consenting 12,928 healthy hospital employees antibodies to SARS-CoV-2 could be determined and compared to participant sick leave records. Subjects with viral serum antibodies were not at excess risk for future sick leave (adjusted odds ratio (OR) controlling for age and sex: 0.85 [95% confidence interval (CI) (0.85 (0.43–1.68)]. By contrast, subjects with antibodies had an excess risk for sick leave in the weeks prior to testing [adjusted OR in multivariate analysis: 3.34 (2.98–3.74)]. Thus, presence of viral antibodies marks past disease and protection against excess risk of future disease. Knowledge of whether exposed subjects have had disease in the past or are at risk for future disease is essential for planning of control measures.Trial registration: First registered on 02/06/20, ClinicalTrials.gov NCT04411576.