bluuebunny/crossref_metadata_2025_split huggingface dataset:
ARTIGO DE REVISÃO: NOVAS OPÇÕES DE TRATAMENTO DO MELANOMA METASTÁTICO
Authors: Costa Milena Katrini , Soares Rodrigo Mesquita , Fagundes Fabiana Ribeiro Queiroz de Oliveira , Guedes Virgílio Ribeiro 
Published: May 2021

RESUMO
O Melanoma é considerado um tumor agressivo, tem sua origem nos melanócitos da pele. Tal tumor tem grande potencial de produzir metástases, e por isso tem grande letalidade. O presente trabalho tem objetivo de fazer uma revisão da literatura sobre as novas terapias da forma metastática do melanoma. Buscou-se artigos através das plataformas SciELO, Pubmed e Bireme, com as seguintes palavras-chave: “melanoma”, “tratamento melanoma”, “treatment melanoma”, “metastatic melanoma”. Os recentes estudos mostram evidências positivas nas pesquisas, atestando a evolução da terapia, passando desde a quimioterapia, inibidores das vias de sinalização, imunoterapia, alternativas naturais até as combinações de terapias. É possível perceber a evolução do tratamento dessa enfermidade, observando a eficácia dos novos métodos e a elevação da sobrevida dos pacientes por meio dos artigos analisados.
Palavras-chave: melanoma; metástase; tratamento.
ABSTRACT
Melanoma is considered an aggressive tumor, has its origin in melanocytes of the skin. It has a great potential to produce metastases, and therefore has great lethality. The present work aims to review the literature on the new therapies of the metastatic form of melanoma. We searched through the SciELO, Pubmed and Bireme platforms with the following keywords: "melanoma", "melanoma treatment", "treatment melanoma", "metastatic melanoma". Recent studies show positive evidence in research, attesting the evolution of therapy, ranging from chemotherapy, inhibitors of signaling pathways, immunotherapy, natural alternatives to combinations of therapies. It is possible to perceive the evolution of the treatment of this disease, observing the efficacy of the new methods and the increase of the patients survival through the articles analyzed.
Keywords: melanoma; metastasis; treatment.
DIAGNÓSTICO PRECOCE DE HANSENÍASE E AÇÕES ESTRATÉGICAS PARA A SUA DETECÇÃO
Authors: Stafin Inês , Guedes Virgílio Ribeiro , Mendes Seyna Ueno Rabelo 
Published: May 2021

RESUMO
Introdução: O diagnóstico da hanseníase ainda causa grande impacto social, tanto por ser frequentemente tardio e evoluir com sequelas, quanto pelos estigmas que a envolvem. O Brasil persiste como uma área endêmica e possui o segundo lugar em número de casos da doença no mundo. Já o Tocantins é um dos estados com maior endemicidade no país, com um aumento importante no número de diagnósticos em menores de 15 anos e no número de pessoas diagnosticadas com incapacidade grau II. Metodologia: Trata-se de uma revisão de literatura do tipo narrativa, realizada por meio de publicações sobre as ações estratégicas para o diagnóstico precoce de hanseníase, entre o período de 2012 a 2017. Desenvolvimento: A situação atual da hanseníase demonstra a persistência da transmissão, a carência de ações para o seu controle efetivo, a sub detecção e um diagnóstico tardio dessa patologia. O presente artigo aborda as principais ações estratégicas para a realização do diagnóstico precoce da hanseníase, bem como os métodos diagnósticos importantes, proporcionando um maior auxílio aos profissionais de saúde da atenção primária. Considerações finais: Para que se amplie o diagnóstico precoce de forma adequada é essencial a busca e avaliação dos contatos, a qualificação adequada dos profissionais de saúde, a educação em saúde da população e a participação ativa dos gestores.
Palavras-chave: Hanseníase; diagnóstico precoce; estratégias; atenção primária.
ABSTRACT
Introduction: The diagnosis of leprosy still causes great social impact, both because it is often late and evolves with sequelae, and because of the stigmas that surround it. Brazil persists as an endemic area and has the second largest number of cases of the disease in the world. The state of Tocantins represents one of the areas with high endemicity in the country, with a significant increase in the number of diagnoses in children under 15 years and in the number of people diagnosed with disability grade II. Methodology: This is a literature review of the narrative type, carried out through publications on strategic actions for the early diagnosis of leprosy, between the period of 2012 to 2017. Development: The current situation of leprosy shows the persistence of transmission, lack of actions for effective control, sub detection and a late diagnosis of this pathology. This article discusses the main strategic actions for the early diagnosis of leprosy, as well as the important diagnostic methods, providing greater assistance to primary health care professionals. Final considerations: In order to increase the early diagnosis in an adequate way, it is essential the search and evaluation of contacts, the adequate qualification of health professionals, the health education of the population and the participation of managers.
Keywords: Leprosy; early diagnosis; strategies; primary care.
PERFIL CLÍNICO E CITOPATOLÓGICO CONTIDO EM AMOSTRAS DE PUNÇÃO ASPIRATIVA POR AGULHA FINA DE NÓDULOS TIREOIDIANOS
Authors: Lima Silveira Edvaldo Lima , Santos Silveira Yan 
Published: May 2021

RESUMO
A malignidade de nódulos tireoidianos é identificada em aproximadamente 10% dos indivíduos e, apesar de pouco frequente, é a principal causa de neoplasia endócrina. Variáveis clínicas como idade, gênero e achados ultrassonográficos são determinantes ao risco de malignidade, e tal determinação é peculiar para cada população estudada, ficando evidente a necessidade de se avaliar tais variáveis em níveis regionais. A partir disso, o estudo buscou investigar o perfil clínico e citopatológico de amostras de Punção Aspirativa por Agulha Fina de nódulos tireoidianos em um laboratório particular de Belém-PA, recebidas entre janeiro de 2016 a dezembro de 2017. Para isso, o estudo contou com a análise de 486 resultados de amostras de Punção Aspirativa por Agulha Fina (PAAF) de nódulos tireoidianos. A maioria das amostras analisadas era de indivíduos do sexo feminino (92,4%), entre 46-65 anos (47%) e com níveis normais de TSH (83,5%). O tamanho do nódulo e a ecogenicidade apresentou influência sobre os grupos de amostras benignas (93,6%) e não benignas (6,4%). Conclui-se que é fundamental a busca pelos perfis de cada população individualmente, identificando quais fatores estão relacionados à maior ou menor risco de malignidade.
Palavras-chave: Nódulo da glândula tireoide; Citopatologia; Ultrassonografia; Biópsia por agulha.
ABSTRACT
The malignancy of thyroid nodules is identified in approximately 10% of the individuals and, although infrequent, is the main cause of endocrine neoplasia. Clinical variables such as age, gender and ultrasonographic findings are determinant to the risk of malignancy, and this determination is peculiar to each population, making evident the need to evaluate such variables at regional levels. From this, the study investigated the clinical and cytopathological profile in samples of Fine-needle Aspiration of thyroid nodules in a private laboratory from Belém-Pará, received between January 2016 and December 2017. For this, the study analyzed 486 results of Fine-needle Aspiration Biopsy of thyroid nodules. Most of the samples analyzed were female (92,4%), 46-65 years old (47%) and normal TSH levels (83,5%). The nodule size and echogenicity had an influence on groups of benign samples (93,6%) and non-benign samples (6,4%). It is fundamental to search for the profiles of each population individually, identifying which factors are related to the greater or lesser risk of malignancy.
Keywords: Thyroid nodules; Pathology; Ultrasonography; Biopsy, Fine-Needle.
RELATO DE CASO: SÍNDROME DO DESFILADEIRO TORÁCICO
Authors: Gonçalves Tatiane Silva , De Sá Raíssa Nunes Bezerra , Pacheco Jéssica Neto Ferreira , Leite Alexis Alison Cardozo , Cuellar Pedro Manuel Gonzales 
Published: May 2021

RESUMO
Introdução: A Síndrome do Desfiladeiro Torácico (SDT) é uma entidade clínica com sintomatologia diversa, decorrente de compressão anormal do plexo braquial, na região do desfiladeiro torácico. Esta compressão é exercida, na maioria das vezes, pelo músculo escaleno anterior, mas pode resultar também da existência de bandas musculofibróticas, alteração da morfologia da primeira costela, costelas cervicais e músculos anômalos. A SDT pode ser classificada nos tipos vascular e neurogênico. Descrição do caso: Paciente, sexo feminino, 27 anos, com quadro de dor e parestesia, há dois anos, de início insidioso, em 4º e 5º quirodáctilos e, posteriormente, com progressão para todo membro superior esquerdo (MSE). Procurou Unidade de Pronto Atendimento, sendo prescrito apenas medicação analgésica. Após três dias, apresentou intensificação da dor, associada a palidez e parestesia de MSE procurando atendimento no Hospital Geral de Palmas. Discussão: A SDT acomete mais mulheres entre 20-50 anos, com vários fatores contribuintes, chegando a limitar as atividades diárias e laborais. O tratamento clínico, frequentemente, é a conduta inicial, procurando aliviar os sintomas. Em geral, o tratamento cirúrgico tem indicação em 15 % dos casos, quando a síndrome é decorrente de anomalias ósseas sintomáticas e complicações vasculares.
Palavras-chave: Síndrome do Desfiladeiro Torácico; Costela Cervical; Plexo Braquial.
ABSTRACT
Introduction: Thoracic Outlet Syndrome (TOS) is a clinical entity with diverse symptomatology due to abnormal compression of brachial plexus in the thoracic outlet region. This compression is often carried out by the anterior scalene muscle, but it may be a result from the presence of musculofibrotic bands, alteration of the first rib morphology, cervical ribs and anomalous muscles. TOS can be classified into vascular and neurogenic types. Case description: Patient, female, 27 years old, with pain and paresthesia, since two years ago, insidious onset, in 4th and 5th fingers, and later with progression to all left upper limb (LUL). She looked for Emergency Care Unit, and only analgesic medication was prescribed. After three days, she presented pain intensification, associated with pallor and paresthesia of LUL, looking for care at the General Hospital of Palmas. Discussion: The TOS affects more women between 20-50 years old, with several contributing factors, limiting daily activities and work. The clinical treatment, often, is the initial conduct, seeking to relieve symptoms. In general, the surgical treatment is indicated in 15% of cases, when the syndrome is due to symptomatic bone anomalies and vascular complications.
Keywords: Thoracic Outlet Syndrome; Cervical Rib; Brachial Plexus.
CONGENITAL CENTRAL HYPOVENTILATION SYNDROME ASSOCIATED WITH HIRSCHSPRUNG’S DISEASE
Authors: De Lima Daniel Jhonatas Silvério , De Pina Tavares Nadine Nair , Alcantara Lucas dos Santos , Pereira Lúcia Caetano , Do Amaral Andrea Silva 
Published: May 2021

RESUMO
A síndrome de hipoventilação central congênita (SHCC) é uma desordem genética rara, associada a mutações no gene paired-like homeobox 2B (PHOX2B), caracterizada por disautonomia e apneia central. A doença de Hirschsprung (DH) está presente em cerca de 20% dos casos de SHCC. Este estudo objetiva relatar o caso de um neonato que apresentou apneia central recorrente, eliminação tardia de mecônio e distensão abdominal diagnosticado com DH associada a SHCC, comparando-se os aspectos clínicos esperados e os encontrados pela equipe que assistiu a criança. Nota-se um retardo no diagnóstico principalmente pela raridade da síndrome e ao seu desconhecimento por parte dos profissionais da saúde. Sendo assim, conclui-se ser imprescindível medidas de suporte, diagnóstico e tratamento precoces pelo risco de complicações graves que ambas as doenças podem acarretar ao recém nascido.
Palavras-chave: Síndrome de hipoventilação central congênita, síndrome de Ondine, gene PHOX2B, doença de Hirschsprung, síndrome de Haddad.
ABSTRACT
Congenital central hypoventilation syndrome (CCHS) is a rare genetic disorder associated with mutations in paired-like homeobox 2B gene (PHOX2B), characterized by dysautonomia and central sleep apnea. Hirschsprung's disease (HD) is present in about 20% of CCHS cases. This study aims to report the case of a neonate who presented with sleep apnea, late elimination of meconium and abdominal distension diagnosed with CCHS associated with HD, comparing the clinical aspects expected and those found by the medical team that attended the child. There was a delay in the diagnosis, mainly due to the rarity of the syndrome and the lack of knowledge about the disease by health professionals. Therefore, it is concluded that life support, early diagnosis, and treatment are essential due to the risk of serious complications that both diseases can cause to the newborn.
Keywords: Congenital central hypoventilation syndrome, Ondine’s curse, PHOX2B gene, Hirschsprung's disease, Haddad syndrome.
Identification of epigenetic readers by DAPPL
Authors: Song Guang , Wang Guohua , Luo Ximei , Cheng Ying , Song Qifeng , Wan Jun , Moore Cedric , Song Hongjun , Jin Peng , Qian Jiang , Zhu Heng 
Published: May 2021

Abstract
Identifying readers of epigenetic marks on DNA is a critical step towards understanding the mechanisms of those modifications in many biological processes. Here, we present an all-to-all approach, dubbed digital affinity profiling via proximity ligation (DAPPL), to simultaneously profile readers for different epigenetic modifications (i.e., 5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine) on CpG dinucleotides using random DNA libraries. Using specific DNA fragments to covalently barcode each human TFs and co-factors, we could connect the identity of a protein to its captured DNA fragments via proximity ligation in a highly multiplexed. Using this approach, we identified numerous readers for different DNA epigenetic modifications
VaNGuard assay protocol for SARS-CoV-2 detection
Authors: Ooi Kean Hean , Liu Mengying Mandy , Tay Jie Wen Douglas , Teo Seok Yee , Kaewsapsak Pornchai , Jin Shengyang , Lee Chun Kiat , Hou Jingwen , Maurer-Stroh Sebastian , Lin Weisi , Yan Benedict , Yan Gabriel , Gao Yong-Gui , Tan Meng How 
Published: May 2021

Abstract
This protocol presents the Variant Nucleotide Guard (VaNGuard) assay, which is robust towards viral mutations and can be performed on purified RNA or directly on nasopharyngeal (NP) swab samples. The procedure typically comprises three parts, namely sample preparation, RT-LAMP reaction, and Cas12a-based detection via fluorescence or lateral flow assay. Sample preparation from NP swabs involves Proteinase K digestion followed by heat inactivation. Purified RNA or digested NP swab samples are then added as templates into RT-LAMP reactions and incubated at 65ºC for 22 minutes. Next, enAsCas12a and ssDNA-probes are added and the reactions are incubated at 60ºC for another 5 minutes. End-point fluorescence can be detected by a plate reader or a real-time PCR machine. Alternatively, a lateral flow strip can be inserted into each reaction tube for equipment-free read-out. The VaNGuard assay is a rapid and convenient point-of-care test for SARS-CoV-2 and is applicable to resource poor settings.
Role of Interferon Therapy in Severe COVID-19: The COVIFERON Randomized Controlled Trial
Authors: Irvani Seyed Sina Naghibi , Golmohammadi Maryam , Pourhoseingholi Mohamad Amin , Shokouhi Shervin , Darazam Ilad Alavi 
Published: May 2021

Abstract
Study Title: Effectiveness of Interferon Beta 1a and Interferon Beta 1b compared to the Usual Therapeutic Regimen to Treat Adults with Moderate to Severe COVID-19Objectives:We will investigate the effectiveness of Interferon Beta 1a, compared to Interferon Beta 1b and the usual therapeutic regimen in COVID-19 in patients that have tested positive and are moderately to severely ill. Trial Design:This is a single center, open label, randomized, controlled, parallel group, clinical trial that will be conducted at Loghman Hakim hospital in conjunction with Shahid Beheshti University of Medical Sciences.
Single cell high-throughput qRT-PCR protocol
Authors: Achanta Sirisha , Vadigepalli Rajanikanth 
Published: May 2021

Abstract
Single cell high-throughput qRT-PCR protocol combines high sensitivity technique of single cell qPCR with high-throughput qPCR technology that can generate data from 96 samples and 96 genes in a single experiment. It can be adapted for various sample types- cell culture, tissue samples and extracted RNA (10 pg) and measured on traditional qPCR and high-throughput qPCR platforms. The workflow is comprised of four steps – cell lysis, reverse transcription, pre-amplification and qPCR. Key features of this protocol are; processing low input samples directly to reverse transcription without RNA extraction which minimizes sample loss, pre-amplification enables amplification of cDNA from single cells to detectable levels for qPCR and measuring up to 400 genes from a single cell sample/10 pg of RNA (starting material). Robust, reproducible and versatile this protocol can be adapted to several upstream and downstream techniques.
Characterization of the bacterioplankton community and the influencing factors in the upper reaches of the Han River basin
Authors: Sun He , Pan Baozhu , He Haoran , Zhao Gengnan , Jiang Xiaoming , Han Xu , Wang Hao 
Published: May 2021

Abstract
The upper reaches of the Han River are the source region of water for the Middle Route of China’s South-to-North Water Diversion Project, mainly for household, industrial, and irrigation purposes. Planktonic bacteria are more sensitive than macroorganisms to water physical and chemical properties and play a critical role in biogeochemical processes in river ecosystems. In November 2017 and April 2018, a systematic and methodical survey was carried out to evaluate the water quality and bacterial communities, on the mainstem of the Han River and its five main tributaries. In this study, high-throughput sequencing technology has been employed to investigate the bacterioplankton community composition. The results indicated that: 1) Diversity increased downstream, especially in the upper reaches of the Han River. 2) The relative abundance of Actinobacteria increased with the increase of river length, while that of Bacteroidetes decreased slightly. 3) Five tributaries were found to be importance sources of taxa to the Han River, however in both months a large proportion of OTUs (37.84% and 36.34%, respectively) had unknown sources. 4) Finally, redundancy analysis (RDA) and Bioenv analysis showed that environmental parameters (pH, TN, Cond, NH4+-N, DO, NO2−-N, Chl-a and T) had a great influence (p ≤ 0.05) on the bacterioplankton community. These research results are beneficial for the managing the ecological system, protecting the tributary biodiversity, and conserving the mainstem and tributaries of the Han River basin.
Anti-Fungal and Anti-Aflatoxigenic Properties of Organs of Cannabis sativa L.: Relation to Phenolic Content and Antioxidant Capacities
Authors: Khoury Anthony Al , Sleiman Rhend , Atoui Ali , Hindieh Pamela , Maroun Richard G , BAILLY Jean-Denis , Khoury André 
Published: May 2021

Abstract
Aflatoxin B1 is a carcinogenic mycotoxin that frequently contaminates crops worldwide. Current research indicates that the use of natural extracts to combat mycotoxin contamination may represent an eco-friendly, sustainable strategy to ensure food safety. Although Cannabis sativa L. has long been known for its psychoactive cannabinoids, it is also rich in many other bioactive molecules. This study examines extracts from various organs of Cannabis sativa L. to determine their ability to limit aflatoxin production and growth of Aspergillus flavus. The results indicate that flower extract is most effective for limiting the synthesis of aflatoxin B1, leading to an almost-complete inhibition of toxin production at a concentration of 0.225 milligrams dry matter per gram of culture medium. Since flower extract is rich in phenolic compounds, its total antioxidant ability and radical-scavenging capacity are determined. Compared with other anti-aflatoxigenic extracts, the anti-oxidative potential of Cannabis sativa L. flower extract appears moderate, suggesting that its anti-mycotoxin effect may be related to other bioactive compounds.
A recessive gene pepy-1 encoding Pelota confers resistance to begomovirus isolates of PepYLCIV and PepYLCAV in Capsicum annuum
Authors: Koeda Sota , Onouchi Mika , Mori Namiko , Pohan Nadya , Nagano Atsushi J. , Kesumawati Elly 
Published: May 2021

Abstract
Pepper yellow leaf curl disease caused by begomoviruses seriously affects pepper (Capsicum spp.) production in a number of regions around the world. Ty genes of tomato, which confer resistance to the tomato yellow leaf curl virus, are the only begomovirus resistance genes cloned to date. In this study, we focused on the identification of begomovirus resistance genes in Capsicum annuum. BaPep-5 was identified as a novel source of resistance against pepper yellow leaf curl Indonesia virus (PepYLCIV) and pepper yellow leaf curl Aceh virus (PepYLCAV). A single recessive locus, which we named as pepper yellow leaf curl disease virus resistance 1 (pepy-1), responsible for PepYLCAV resistance in BaPep-5 was identified on chromosome 5 in an F2 population derived from a cross between BaPep-5 and the begomovirus susceptible accession BaPep-4. In the target region spanning 34 kb, a single candidate gene, the messenger RNA surveillance factor Pelota, was identified. Whole-genome resequencing of BaPep-4 and BaPep-5 and comparison of their genomic DNA sequences revealed a single nucleotide polymorphism (A to G) located at the splice site of the 9th intron of CaPelota in BaPep-5, which caused the insertion of the 9th intron into the transcript, resulting in the addition of 28 amino acids to CaPelota protein without causing a frameshift. Virus-induced gene silencing of CaPelota in the begomovirus susceptible pepper No.218 resulted in the gain of resistance against PepYLCIV, a phenotype consistent with BaPep-5. The DNA marker developed in this study will greatly facilitate marker-assisted breeding of begomovirus resistance in peppers.