bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERENCANAAN DAN PENGENDALIAN PERSEDIAAN ALUMINIUM SULFAT CAIR DENGAN METODE EOQ STUDI KASUS PADA PDAM TIRTA MAYANG KOTA JAMBI
Authors: Dewi Diana Chandra 
Published: March 2021

Penelitian ini bertujuan untuk mengetahui bagaimana Perencanan Dan Pengendalian Aluminium Sulfat Cair pada PDAM Tirta Mayang Kota Jambi dengan menggunakan metode EOQ perusahaan dapat menghasilkan biaya yang lebih murah ketika disandingkan dengan teknik yang diterapkan perusahaan selama ini. Penghematan yang didapatkan jika metode EOQ diaplikasikan pada perusahaan pada tahun 2019 yaitu senilai Rp 12.039.812,- . Berdasarkan hasil peneltian, persediaan material dengan menggunakan metode Economic Order Quantity dan total biaya persediaan bahan baku dengan metode EOQ hasil yang didapatkan telah efisien sehingga perusahaan dapat mempertimbangkan untuk menggunakan metode EOQ dalam mengadakan persediaan bahan baku aluminium sulfat cair.
Новації у митному законодавстві України
Authors: Несторишен І. В. , Туржанський В. А. , Бережнюк І. Г. 
Published: March 2021

У статті узагальнено вітчизняну практику здійснення процедур постмитного контролю, а також обґрунтовано доцільність внесення змін до Митного кодексу України щодо запровадження інструментів постмитного контролю у вітчизняне митне законодавство. В основу дослідження покладено концепцію збалансування інтересів держави та суб’єктів зовнішньоекономічної діяльності щодо забезпечення національної безпеки та спрощення процедур митного контролю. Митний контроль після випуску є складовою частиною системи надання спрощень, який дозволяє забезпечити мінімальне втручання митних органів в зовнішньоекономічну діяльність бізнесу з високим ступенем довіри.
Одним з актуальних питань у сфері здійснення державної митної справи в Україні є наближення митного законодавства до положень міжнародних конвенцій та угод, а також в умовах реалізації подальших євроінтеграційних прагнень гармонізація вітчизняного інструментарію здійснення митного контролю із європейським. Зазначено, що 2016 р. в Європейському Союзі набув чинності ряд нормативних документів, які істотно змінили підходи до здійснення процедур митного контролю та порядок надання спрощень економічним операторам. Зокрема, Митним кодексом ЄС передбачено активне використання інструментів митного контролю після випуску (post-release control), який є одним із складових частин системи надання спрощень економічним операторам. В Україні, не зважаючи на передбачене нормативне підґрунтя контролю після випуску, ця форма митного контролю не працює повною мірою, а показники ефективності підрозділів митного аудиту Держмитслужби є низькими. Авторами проаналізовано нормативні документи ЄС, якими передбачено використання інструментів митного контролю після випуску в контексті внесення змін до Митного кодексу України, та узагальнено новації у вітчизняному митному законодавстві відповідно до положень митного законодавства ЄС.
Madecassoside Inhibits Body Weight Gain via Modulating SIRT1-AMPK Signaling Pathway and Activating Genes Related to Thermogenesis
Authors: Sun Boju , Hayashi Misa , Kudo Maya , Wu Lili , Qin Lingling , Gao Ming , Liu Tonghua 
Published: March 2021

BackgroundPre-clinical research studies have shown that Madecassoside (MA) has favorable therapeutic effects on arthritis, acne, vitiligo and other diseases. However, the effects of MA on obesity have not yet been studied. This study mainly aimed to investigate the effects of MA in protecting against obesity and its underlying mechanism in reducing obesity.MethodsObese diabetic KKay/TaJcl mice model was adopted to the study. The body weight of all animals was recorded daily, and the blood glucose, blood lipid, and serum aminotransferase levels were examined, respectively. The expression of P-AMPK, SIRT1, P-LKB1, P-ACC, and P-HSL in abdominal fat, mesenteric fat, and epididymal fat was measured by western blotting, and the levels of PPARα, CPT1a, PGC-1α, UCP-1, Cidea, Cox7a1, and Cox8b were examined by real-time quantitative PCR (RT-qPCR).ResultsThe results revealed that the body weight of the mice in MA group was significantly reduced, and the body mass index (BMI) showed significant difference between the two groups after 8 weeks of MA treatment. Further research revealed that it affected the mesenteric fat and epididymis fat by activating SIRT1/AMPK signaling pathway, and then promoted fatty acid oxidation of epididymal fat (PPARα ↑, CPT1a↑, and PGC-1α↑). Last but not the least, it also promoted the expression of UCP-1 and stimulated thermoregulatory genes (Cidea, Cox7a1, and Cox8b) in brown fat and mesenteric fat.ConclusionsTaken together, these findings suggest that MA can inhibit the weight gain in obese diabetic mice, and reduce triglyceride levels, inhibit lipogenesis of mesenteric fat, promote epididymal fat lipolysis and fatty acid oxidation. Furthermore, MA treatment might promote mesenteric fat browning and activate mitochondrial function in brown fat as well as mesenteric fat.
Sperm DNA Methylation at Metabolism-Related Genes in Vegan Subjects
Authors: Franzago Marica , Sabovic Iva , Franchi Sara , De Santo Maria , Di Nisio Andrea , Luddi Alice , Piomboni Paola , Vitacolonna Ester , Stuppia Liborio , Foresta Carlo 
Published: March 2021

ObjectiveTo investigate if epigenome of sperm cells could be dynamically affected by nutrition.Design and MethodsWe assessed 40 healthy volunteers with different dietary habits and collected their demographic characteristics, as well as clinical and anthropometric parameters. We compared methylation profiles in sperm quantified by bisulfite pyrosequencing, at promoter-associated CpG sites of genes involved in metabolism including fat mass and obesity-associated (FTO) and melanocortin-4 receptor (MC4R) from six vegans and 34 omnivores. In addition, the FTO rs9939609 (T&gt;A) was genotyped.ResultsHigher DNA methylation levels were detected in the sperm of vegan at FTO gene CpG1 (p=0.02), CpG2 (p=0.001), CpG3 (p=0.004), and CpG4 (p=0.003) sites and at MC4R-CpG2 site [p=0.016] as compared to sperm of omnivores. This association was not related to FTO genotype.ConclusionsAlthough limited by the small number of investigated cases, our data provide insight into the role of diet on sperm DNA methylation in genes involved in metabolism.
Seasonal and Spatial Detection of Pesticide Residues Under Various Weather Conditions of Agricultural Areas of the Kilombero Valley Ramsar Site, Tanzania
Authors: Materu Silvia F. , Heise Susanne , Urban Brigitte 
Published: March 2021

This study was conducted in the Kilombero Valley Ramsar Site (KVRS), the largest low-altitude freshwater wetland in East Africa to: 1) determine pesticide contamination of soil, water, and sediment at different seasonal weather conditions 2) critically analyze the seasonal and spatial ecological impacts of pesticide contaminations under tropical weather conditions 3) estimate the potential environmental risks from pesticide exposure based on analytical data. Surface sediments, soil and water samples were collected from sugarcane, teak and rice plantations of the KVRS during the dry (January 2012) and the rainy (March–April 2013) seasons. Samples were screened for 97 different types of pesticide residues using and LC–MS and HPLC–MS. Physical-chemical properties of the sediment and soil samples and meteorological data were analyzed. 92% of soil and sediment samples had relatively low OM content (&lt;5%) while 60% of samples had high clay content, indicating high cation exchange capacity that can bind pesticides to the soils. Field soil and air temperature ranging 28–35°C, total sunshine hours-208 h/month, photo radiation −764 MJ/m2, average precipitation 300 mm/month, wind speed &gt;60 km/h, humidity &gt;80% were recorded. Frequency of detection and concentration of pesticides residues depended on seasons. For the dry season sampling, glyphosate/AMPA (0.01–0.55 µg/g), and diuron (0.026–1.7 µg/g) were mostly detected in the sediment and soils. Monuron (1.28–3.9 µg/L) and propoxur (0.05–0.6 µg/L) were mostly detected in the rainy seasons (water and sediments). Glyphosate was above limit of detection in the dry season water samples indicating that its emission into the water system may have been directly through application. Maximum diuron concentrations in water were &gt;30 times higher than the estimated predicted no-effect concentration (PNEC) value (0.054 µg/L) indicating a risk to aquatic community. Calculated pore water concentrations (0.992–0.081 µg/L), exceeded the estimated PNEC values during the dry season, indicating a risk to benthic organisms. Detection of pesticide is influenced by application, environmental factors and the timing of sampling. Further research is recommended to increase our knowledge on soil-water-sediment interactions in the tropics with regard to pesticides.
Blood Interferon-α Levels and Severity, Outcomes, and Inflammatory Profiles in Hospitalized COVID-19 Patients
Authors: Contoli Marco , Papi Alberto , Tomassetti Luca , Rizzo Paola , Vieceli Dalla Sega Francesco , Fortini Francesca , Torsani Francesca , Morandi Luca , Ronzoni Luca , Zucchetti Ottavio , Pavasini Rita , Fogagnolo Alberto , Volta Carlo Alberto , Bartlett Nathan W. , Johnston Sebastian L. , Spadaro Savino , Campo Gianluca 
Published: March 2021

Background: Deficient interferon responses have been proposed as one of the relevant mechanisms prompting severe manifestations of COVID-19.Objective: To evaluate the interferon (IFN)-α levels in a cohort of COVID-19 patients in relation to severity, evolution of the clinical manifestations and immune/inflammatory profile.Methods: This is prospective study recruiting consecutive hospitalized patients with respiratory failure associated with SARS-COV-2 infection and matched controls. After enrollment, patients were assessed every 7 ± 2 days for additional 2 consecutive visits, for a total of 21 days. The severity of the clinical condition was ranked based on the level of respiratory support required. At each time-point blood samples were obtained to assess immune cells and mediators by multiplex immunoassay.Results: Fifty-four COVD-19 and 11 control patients matched for severity were enrolled. At recruitment, lower levels of blood IFN-α were found in COVID-19 patients compared to controls (3.8-fold difference, p &lt; 0.01). Improvements in COVID-19 severity were paralleled by a significant increase of blood IFN-α levels. A significant increase in blood IFN-α was found over the study period in survivors (70% of the study population). A similar trend was found for blood IFN-β with IFN-β levels below the threshold of detectability in a substantial proportion of subjects. Significantly higher values of blood lymphocytes and lower levels of IL-10 were found at each time point in patients who survived compared to patients who died. In patients who clinically improved and survived during the study, we found an inverse association between IL-10 and IFN-α levels.Conclusion: The study identifies a blood immune profile defined by deficient IFN-α levels associated with increased IL-10 expression in patients progressing to severe/life threatening COVID-19 conditions, suggesting the involvement of immunological pathways that could be target of pharmacological intervention.Clinical Trial Registration:ClinicalTrials.gov identifier NCT04343053.
Patient Preferences to Assess Value IN Gene Therapies: Protocol Development for the PAVING Study in Hemophilia
Authors: van Overbeeke Eline , Hauber Brett , Michelsen Sissel , Goldman Michel , Simoens Steven , Huys Isabelle 
Published: March 2021

Introduction: Gene therapies are innovative therapies that are increasingly being developed. However, health technology assessment (HTA) and payer decision making on these therapies is impeded by uncertainties, especially regarding long-term outcomes. Through measuring patient preferences regarding gene therapies, the importance of unique elements that go beyond health gain can be quantified and inform value assessments. We designed a study, namely the Patient preferences to Assess Value IN Gene therapies (PAVING) study, that can inform HTA and payers by investigating trade-offs that adult Belgian hemophilia A and B patients are willing to make when asked to choose between a standard of care and gene therapy.Methods and Analysis: An eight-step approach was taken to establish the protocol for this study: (1) stated preference method selection, (2) initial attributes identification, (3) stakeholder (HTA and payer) needs identification, (4) patient relevant attributes and information needs identification, (5) level identification and choice task construction, (6) educational tool design, (7) survey integration, and (8) piloting and pretesting. In the end, a threshold technique survey was designed using the attributes “Annual bleeding rate,” “Chance to stop prophylaxis,” “Time that side effects have been studied,” and “Quality of Life.”Ethics and Dissemination: The Medical Ethics Committee of UZ KU Leuven/Research approved the study. Results from the study will be presented to stakeholders and patients at conferences and in peer-reviewed journals. We hope that results from the PAVING study can inform decision makers on the acceptability of uncertainties and the value of gene therapies to patients.
Application of Machine Learning Algorithms to Predict Central Lymph Node Metastasis in T1-T2, Non-invasive, and Clinically Node Negative Papillary Thyroid Carcinoma
Authors: Zhu Jiang , Zheng Jinxin , Li Longfei , Huang Rui , Ren Haoyu , Wang Denghui , Dai Zhijun , Su Xinliang 
Published: March 2021

Purpose: While there are no clear indications of whether central lymph node dissection is necessary in patients with T1-T2, non-invasive, clinically uninvolved central neck lymph nodes papillary thyroid carcinoma (PTC), this study seeks to develop and validate models for predicting the risk of central lymph node metastasis (CLNM) in these patients based on machine learning algorithms.Methods: This is a retrospective study comprising 1,271 patients with T1-T2 stage, non-invasive, and clinically node negative (cN0) PTC who underwent surgery at the Department of Endocrine and Breast Surgery of The First Affiliated Hospital of Chongqing Medical University from February 1, 2016, to December 31, 2018. We applied six machine learning (ML) algorithms, including Logistic Regression (LR), Gradient Boosting Machine (GBM), Extreme Gradient Boosting (XGBoost), Random Forest (RF), Decision Tree (DT), and Neural Network (NNET), coupled with preoperative clinical characteristics and intraoperative information to develop prediction models for CLNM. Among all the samples, 70% were randomly selected to train the models while the remaining 30% were used for validation. Indices like the area under the receiver operating characteristic (AUROC), sensitivity, specificity, and accuracy were calculated to test the models' performance.Results: The results showed that ~51.3% (652 out of 1,271) of the patients had pN1 disease. In multivariate logistic regression analyses, gender, tumor size and location, multifocality, age, and Delphian lymph node status were all independent predictors of CLNM. In predicting CLNM, six ML algorithms posted AUROC of 0.70–0.75, with the extreme gradient boosting (XGBoost) model standing out, registering 0.75. Thus, we employed the best-performing ML algorithm model and uploaded the results to a self-made online risk calculator to estimate an individual's probability of CLNM (https://jin63.shinyapps.io/ML_CLNM/).Conclusions: With the incorporation of preoperative and intraoperative risk factors, ML algorithms can achieve acceptable prediction of CLNM with Xgboost model performing the best. Our online risk calculator based on ML algorithm may help determine the optimal extent of initial surgical treatment for patients with T1-T2 stage, non-invasive, and clinically node negative PTC.
Wnt7a Promotes the Occurrence and Development of Colorectal Adenocarcinoma
Authors: Li Congcong , Dou Xiaowei , Sun Jiahuan , Xie Min , Li Hongli , Cui Peilin 
Published: March 2021

ObjectiveThe expression of Wnt7a in colorectal cancer tissues and cell lines was analyzed, and the effect of Wnt7a on the proliferation of colorectal cancer cells was studied, so as to confirm the relationship between Wnt7a and the occurrence and development of colorectal cancer.Methods(1) Immunohistochemical method was used to compare the expression of Wnt7a in different tissues and its relationship with the clinicopathology of colorectal adenocarcinoma. (2) The expression levels of Wnt7a in colorectal cancer cell lines HT-29 and HCT 116 were detected by qRT-PCR. (3) The down-regulated Wnt7A expression vector was constructed, and the down-regulated Wnt7A expression cell line was established. The regeneration ability of cancer cells was detected by stem cell ball formation assay, and the influence of plate cloning assay on the proliferation ability of colorectal cancer cells was detected.Results(1) The positive rates of Wnt7a in normal colorectal mucosa, colorectal adenoma and colorectal adenocarcinoma tissues gradually increased,Wnt7a are closely related to the degree of colorectal adenocarcinoma differentiation, lymph node metastasis and Duke stage. (2) The expression level of Wnt7a in colorectal cancer cells was higher than that in normal colorectal epithelial cells. (3) The down-regulation of Wnt7A reduced the proliferation ability of colorectal cancer cells.ConclusionsWnt7a promotes the occurrence and development of colorectal adenocarcinoma.
Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma
Authors: Chen Hongjin , Xia Ruixue , Jiang Long , Zhou Yong , Xu Haojun , Peng Weiwei , Yao Chengyun , Zhou Guoren , Zhang Yijie , Xia Hongping , Wang Yongsheng 
Published: March 2021

BackgroundThe Rho GTPase family with ~20 member genes play central roles in a wide variety of cellular processes and tumor cell migration and metastasis. Different Rho GTPase may play different roles in the progression of lung adenocarcinoma.MethodsWe comprehensively examined the expression of all Rho GTPase family member genes in a panel of lung adenocarcinoma patient’s tumors and matched normal tissues. We next investigated the critical role of RhoV in different lung adenocarcinoma cells and animal models.ResultsRhoV was identified as one of the most significantly overexpressed Rho GTPases in lung adenocarcinoma and associated with patients’ survival. Silencing RhoV expression inhibits proliferation, migration and invasion, and tumorigenicity capacities of lung adenocarcinoma cells. Moreover, knockdown RhoV promoted the sensitivity of EGFR-TKI in the gefitinib resistant PC9 cells (PC9-GR) and aggravated gefitinib-induced lung cancer cell apoptosis both in PC9 and PC9-GR cells. Our data also indicated that RhoV induced progression and EGFR-TKI resistance of lung adenocarcinoma may be related to the activation of the AKT/ERK pathway.ConclusionOverexpression of RhoV in lung adenocarcinoma promotes the progression and EGFR-TKI resistance, suggesting RhoV is a promising prognosis and therapeutic target of lung adenocarcinoma.
Hepatic Steatosis Predicts Higher Incidence of Recurrence in Colorectal Cancer Liver Metastasis Patients
Authors: Chen Haiyan , Dai Siqi , Fang Yimin , Chen Liubo , Jiang Kai , Wei Qichun , Ding Kefeng 
Published: March 2021

Purpose: Colorectal liver metastasis (CRLM) is the major cause of death due to colorectal cancer. Although great efforts have been made in treatment of CRLM, about 60–70% of patients will develop hepatic recurrence. Hepatic steatosis was reported to provide fertile soil for metastasis. However, whether hepatic steatosis predicts higher incidence of CRLM recurrence is not clear. Therefore, we aimed to determine the role of hepatic steatosis in CRLM recurrence in the present study.Methods: Consecutive CRLM patients undergoing curative treatment were retrospectively enrolled and CT liver-spleen attenuation ratio was used to detect the presence of hepatic steatosis. In patients with hepatic steatosis, we also detected the presence of fibrosis. Besides, a systematic literature search was performed to do meta-analysis to further analyze the association between hepatic steatosis and CRLM recurrence.Results: A total of 195 eligible patients were included in our center. Patients with hepatic steatosis had a significantly worse overall (P = 0.0049) and hepatic recurrence-free survival (RFS) (P = 0.0012). Univariate and multivariate analysis confirmed its essential role in prediction of RFS. Besides, hepatic fibrosis is associated with worse overall RFS (P = 0.039) and hepatic RFS (P = 0.048). In meta-analysis, we included other four studies, with a total of 1,370 patients in the case group, and 3,735 patients in the control group. The odds ratio was 1.98 (95% CI: 1.25–3.14, P = 0.004), indicating that patients with steatosis had a significantly higher incidence of CRLM recurrence.Conclusion: In summary, patients with hepatic steatosis had a significantly worse overall and hepatic RFS and it's associated with higher incidence of CRLM recurrence.
Peritumoral Edema Is Associated With Postoperative Hemorrhage and Reoperation Following Vestibular Schwannoma Surgery
Authors: Guo Xiaoming , Zhu Yueli , Wang Xiaoyu , Xu Ke , Hong Yuan 
Published: March 2021

Background: Postoperative hemorrhage (POH) is a severe complication following vestibular schwannoma surgery that may require surgical treatment. The purpose of our study is to identify risk factors associated with POH and reoperation following the resection of vestibular schwannoma.Methods: We retrospectively recruited 452 vestibular schwannoma patients treated with retrosigmoid approach. The primary outcome was POH, and the secondary outcome was reoperation for POH. Clinical and radiographic data were compared by performing univariate analysis and logistic regression analysis.Results: Among the 452 patients, 37 patients (8.2%) presented with POH and14 patients (3.1%) required reoperation within a 30-day hospitalization period. The univariate analysis showed that peritumoral edema, tumor diameter &gt;30 mm, severe postoperative hypertension, and length of hospital stay were associated with POH and reoperation for POH. Logistic regression analysis showed that peritumoral edema [odds ratio (OR) 4.042, 95% confident interval (CI) 1.830–8.926, P = 0.001] and tumor diameter &gt;30 mm (OR 3.192, 95% CI 1.421–7.168, P = 0.005) were independent predictive factors for POH. Peritumoral edema (OR 7.071, 95% CI 2.342–21.356, P = 0.001) was an independent predictive factor for reoperation by using logistic regression analysis. Further analysis revealed that larger tumor and incomplete tumor resection were both associated with a higher incidence of peritumoral edema.Conclusion: Peritumoral edema and tumor size are independent risk factors for POH following vestibular schwannoma surgery. And larger hematoma occurs more commonly in tumors with peritumoral edema which may require reoperation. Tumor size and extent of tumor resection are associated with peritumoral edema. Close attention should be paid to high-risk patients especially for those who presented with severe postoperative hypertension.