bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring of seven industrial anaerobic digesters supplied with biochar
Authors: Heitkamp Kerstin , Latorre-Pérez Adriel , Nefigmann Sven , Gimeno-Valero Helena , Vilanova Cristina , Jahmad Efri , Abendroth Christian 
Published: September 2021

Abstract
Background
Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown that multiple conductive materials such as biochar can be supplemented to anaerobic digesters to increase the rate of DIET. However, the industrial applicability, as well as the impact of such supplements on taxonomic profiles, has not been sufficiently assessed to date.

Results
Seven industrial biogas plants were upgraded with a shock charge of 1.8 kg biochar per ton of reactor content and then 1.8 kg per ton were added to the substrate for one year. A joint analysis for all seven systems showed a decreasing trend for the concentration of acetic acid (p < 0.0001), propionic acid (p < 0.0001) and butyric acid (p = 0.0022), which was significant in all cases. Quantification of the cofactor F420 using fluorescence microscopy showed a reduction in methanogenic archaea by up to a power of ten. Methanogenic archaea could grow within the biochar, even if the number of cells was 4 times less than in the surrounding sludge. 16S-rRNA gene amplicon sequencing showed a higher microbial diversity in the biochar particles than in the sludge, as well as an accumulation of secondary fermenters and halotolerant bacteria. Taxonomic profiles indicate microbial electroactivity, and show the frequent occurrence of Methanoculleus, which has not been described in this context before.

Conclusions
Our results shed light on the interplay between biochar particles and microbial communities in anaerobic digesters. Both the microbial diversity and the absolute frequency of the microorganisms involved were significantly changed between sludge samples and biochar particles. This is particularly important against the background of microbial process monitoring. In addition, it could be shown that biochar is suitable for reducing the content of inhibitory, volatile acids on an industrial scale.
First report of interspecific transmission of sarcoptic mange from Iberian ibex to wild boar
Authors: Valldeperes Marta , Moroni Barbara , Rossi Luca , López-Olvera Jorge Ramón , Velarde Roser , Molinar Min Anna Rita , Mentaberre Gregorio , Serrano Emmanuel , Angelone Samer , Lavín Santiago , Granados José Enrique 
Published: September 2021

Abstract
Background
Sarcoptic mange is a globally distributed parasitic disease caused by the burrowing mite Sarcoptes scabiei. This mite has a certain degree of host specificity, although interspecific transmission can occur among phylogenetically related species or through prey–predator mediated exposure. In 2018, a wild boar (Sus scrofa) with lesions compatible with sarcoptic mange was hunted in Ports de Tortosa i Beseit Natural Park (PTB, north-eastern Spain), where an active epizootic outbreak of sarcoptic mange is affecting Iberian ibexes (Capra pyrenaica) since 2014.

Methods
A complete necropsy, skin scrapings and skin digestions with hydroxide potassium were performed to confirm the diagnosis. Routine histopathological analysis, toluidine blue staining and immunohistochemistry were used to characterize the lesions and the inflammatory infiltrate. Finally, 10 specific S. scabiei microsatellites were molecularly genotyped through polymerase chain reactions in mites obtained from the affected wild boar. For phylogenetic comparison, mites obtained from sympatric Iberian ibexes and allopatric wild boars and Iberian ibexes from southern Spain were analysed.

Results
Sarcoptes scabiei was visually and molecularly identified in the infested wild boar from PTB, causing skin lesions with dermal inflammatory infiltrate rich in T and B cells, which indicate an adaptive immune response. Three S. scabiei genetic clusters were identified: one included mites from southern Iberian ibexes, another included mites from southern wild boars, and a third one distinctively grouped the wild boar from PTB with the sympatric ibexes.

Conclusions
To the authors’ knowledge, this is the first reported case of sarcoptic mange in wild boar in Spain and the first documented case of S. scabiei cross-transmission from a wild ruminant host to a wild boar. The wild boar presented an ordinary scabies type reaction, which is typical of the self-limiting infestations reported in other cases of interspecific transmission.

Graphical abstract
Determinants of Aedes mosquito density as an indicator of arbovirus transmission risk in three sites affected by co-circulation of globally spreading arboviruses in Colombia, Ecuador and Argentina
Authors: Talbot Benoit , Sander Beate , Cevallos Varsovia , González Camila , Benítez Denisse , Carissimo Claudio , Carrasquilla Ferro María C. , Gauto Neris , Litwiñiuk Sergio , López Karen , Ortiz Mario I. , Ponce Patricio , Villota Stephany D. , Zelaya Fabian , Espinel Mauricio , Wu Jianhong , Miretti Marcos , Kulkarni Manisha A. 
Published: September 2021

Abstract
Background
The global impact of Zika virus in Latin America has drawn renewed attention to circulating mosquito-borne viruses in this region, such as dengue and chikungunya. Our objective was to assess socio-ecological factors associated with Aedes mosquito vector density as a measure of arbovirus transmission risk in three cities of potentially recent Zika virus introduction: Ibagué, Colombia; Manta, Ecuador; and Posadas, Argentina, in order to inform disease mitigation strategies.

Methods
We sampled Aedes mosquito populations in a total of 1086 households, using indoor and peridomestic mosquito collection methods, including light traps, resting traps, traps equipped with chemical attractant and aspirators. For each sampled household, we collected socio-economic data using structured questionnaires and data on microenvironmental conditions using iButton data loggers.

Results
A total of 3230 female Aedes mosquitoes were collected, of which 99.8% were Aedes aegypti and 0.2% were Aedes albopictus. Mean female Aedes mosquito density per household was 1.71 (standard deviation: 2.84). We used mixed-effects generalized linear Poisson regression analyses to identify predictors of Aedes density, using month, neighborhood and country as random-effects variables. Across study sites, the number of household occupants [incidence rate ratio (IRR): 1.08, 95% confidence interval (CI): 1.01–1.14], presence of entry points for mosquitoes into the household (IRR: 1.51, 95% CI: 1.30–1.76) and presence of decorative vegetation (IRR: 1.52, 95% CI: 1.22–1.88) were associated with higher Aedes density; while being in the highest wealth tertile of household wealth (IRR: 0.78, 95% CI: 0.66–0.92), knowledge of how arboviruses are transmitted (IRR: 0.94, 95% CI: 0.89–1.00) and regular emptying of water containers by occupants (IRR: 0.79, 95% CI: 0.67–0.92) were associated with lower Aedes density.

Conclusions
Our study addresses the complexities of arbovirus vectors of global significance at the interface between human and mosquito populations. Our results point to several predictors of Aedes mosquito vector density in countries with co-circulation of multiple Aedes-borne viruses, and point to modifiable risk factors that may be useful for disease prevention and control.

Graphical Abstract
Anesthesia can alter the levels of corticosterone and the phosphorylation of signaling molecules
Authors: Pereira Gabriela Cruz , Neto Marcos Mônico , Antunes Hanna Karen Moreira , Lee Kil Sun , da Luz Marcio Henrique Mello 
Published: September 2021

Abstract
Objective
Neuroscience research using laboratory animals has increased over the years for a number of reasons. Some of these studies require the use of anesthetics for surgical procedures. However, the use of anesthetics promotes several physiological changes that may interfere with experimental results. Although the anesthetics and methods of delivery used to vary, one of the most common is ketamine associated with another compound such as xylazine. We aimed to evaluate the effect of ketamine and xylazine (KX) on corticosterone levels and on the degree of phosphorylation of p44/42 (ERK1/2), Src kinases and calcium/calmodulin-dependent kinase II (CAMKII). We also compared the effects of KX on sleep deprivation, which is known to affect the hormonal profile including corticosterone.

Results
We found that the use of KX can increase corticosterone levels and alter the degree of phosphorylation of signaling proteins.
DNA 5-hydroxymethylcytosine in pediatric central nervous system tumors may impact tumor classification and is a positive prognostic marker
Authors: Azizgolshani Nasim , Petersen Curtis L. , Chen Youdinghuan , Levy Joshua J. , Salas Lucas A. , Perreard Laurent , Nguyen Lananh N. , Christensen Brock C. 
Published: September 2021

Abstract
Background
Nucleotide-specific 5-hydroxymethylcytosine (5hmC) remains understudied in pediatric central nervous system (CNS) tumors. 5hmC is abundant in the brain, and alterations to 5hmC in adult CNS tumors have been reported. However, traditional approaches to measure DNA methylation do not distinguish between 5-methylcytosine (5mC) and its oxidized counterpart 5hmC, including those used to build CNS tumor DNA methylation classification systems. We measured 5hmC and 5mC epigenome-wide at nucleotide resolution in glioma, ependymoma, and embryonal tumors from children, as well as control pediatric brain tissues using tandem bisulfite and oxidative bisulfite treatments followed by hybridization to the Illumina Methylation EPIC Array that interrogates over 860,000 CpG loci.

Results
Linear mixed effects models adjusted for age and sex tested the CpG-specific differences in 5hmC between tumor and non-tumor samples, as well as between tumor subtypes. Results from model-based clustering of tumors was used to test the relation of cluster membership with patient survival through multivariable Cox proportional hazards regression. We also assessed the robustness of multiple epigenetic CNS tumor classification methods to 5mC-specific data in both pediatric and adult CNS tumors. Compared to non-tumor samples, tumors were hypohydroxymethylated across the epigenome and tumor 5hmC localized to regulatory elements crucial to cell identity, including transcription factor binding sites and super-enhancers. Differentially hydroxymethylated loci among tumor subtypes tended to be hypermethylated and disproportionally found in CTCF binding sites and genes related to posttranscriptional RNA regulation, such as DICER1. Model-based clustering results indicated that patients with low 5hmC patterns have poorer overall survival and increased risk of recurrence. Our results suggest 5mC-specific data from OxBS-treated samples impacts methylation-based tumor classification systems giving new opportunities for further refinement of classifiers for both pediatric and adult tumors.

Conclusions
We identified that 5hmC localizes to super-enhancers, and genes commonly implicated in pediatric CNS tumors were differentially hypohydroxymethylated. We demonstrated that distinguishing methylation and hydroxymethylation is critical in identifying tumor-related epigenetic changes. These results have implications for patient prognostication, considerations of epigenetic therapy in CNS tumors, and for emerging molecular neuropathology classification approaches.
Impact of vitamin C supplementation on placental DNA methylation changes related to maternal smoking: association with gene expression and respiratory outcomes
Authors: Shorey-Kendrick Lyndsey E. , McEvoy Cindy T. , O’Sullivan Shannon M. , Milner Kristin , Vuylsteke Brittany , Tepper Robert S. , Haas David M. , Park Byung , Gao Lina , Vu Annette , Morris Cynthia D. , Spindel Eliot R. 
Published: September 2021

Abstract
Background
Maternal smoking during pregnancy (MSDP) affects development of multiple organ systems including the placenta, lung, brain, and vasculature. In particular, children exposed to MSDP show lifelong deficits in pulmonary function and increased risk of asthma and wheeze. Our laboratory has previously shown that vitamin C supplementation during pregnancy prevents some of the adverse effects of MSDP on offspring respiratory outcomes. Epigenetic modifications, including DNA methylation (DNAm), are a likely link between in utero exposures and adverse health outcomes, and MSDP has previously been associated with DNAm changes in blood, placenta, and buccal epithelium. Analysis of placental DNAm may reveal critical targets of MSDP and vitamin C relevant to respiratory health outcomes.

Results
DNAm was measured in placentas obtained from 72 smokers enrolled in the VCSIP RCT: NCT03203603 (37 supplemented with vitamin C, 35 with placebo) and 24 never-smokers for reference. Methylation at one CpG, cg20790161, reached Bonferroni significance and was hypomethylated in vitamin C supplemented smokers versus placebo. Analysis of spatially related CpGs identified 93 candidate differentially methylated regions (DMRs) between treatment groups, including loci known to be associated with lung function, oxidative stress, fetal development and growth, and angiogenesis. Overlap of nominally significant differentially methylated CpGs (DMCs) in never-smokers versus placebo with nominally significant DMCs in vitamin C versus placebo identified 9059 candidate “restored CpGs” for association with placental transcript expression and respiratory outcomes. Methylation at 274 restored candidate CpG sites was associated with expression of 259 genes (FDR < 0.05). We further identified candidate CpGs associated with infant lung function (34 CpGs) and composite wheeze (1 CpG) at 12 months of age (FDR < 0.05). Increased methylation in the DIP2C, APOH/PRKCA, and additional candidate gene regions was associated with improved lung function and decreased wheeze in offspring of vitamin C-treated smokers.

Conclusions
Vitamin C supplementation to pregnant smokers ameliorates changes associated with maternal smoking in placental DNA methylation and gene expression in pathways potentially linked to improved placental function and offspring respiratory health. Further work is necessary to validate candidate loci and elucidate the causal pathway between placental methylation changes and outcomes of offspring exposed to MSDP.
Clinical trial registration ClinicalTrials.gov, NCT01723696. Registered November 6, 2012. https://clinicaltrials.gov/ct2/show/record/NCT01723696.
Megaloblastic anemia-related iron overload and erythroid regulators: a case report
Authors: Vallet Nicolas , Delaye Jean-Baptiste , Ropert Martine , Foucault Amélie , Ravalet Noémie , Deriaz Sophie , Chalopin Thomas , Blasco Hélène , Maillot François , Hérault Olivier , Gyan Emmanuel 
Published: September 2021

Abstract
Background
In ineffective erythropoiesis, hepcidin synthesis is suppressed by erythroid regulators, namely erythroferrone and growth differentiation factor-15. For the first time, the hypothesis that iron overload in megaloblastic anemia may be related to ineffective erythropoiesis is explored by describing the kinetics of hepcidin, erythroferrone, and growth differentiation factor-15 levels in a patient diagnosed with megaloblastic anemia associated with iron overload.

Case presentation
An 81-year-old Caucasian male was admitted for fatigue. He had type-2 diabetes previously treated with metformin, ischemic cardiac insufficiency, and stage-3 chronic kidney disease. Vitiligo was observed on both hands. Biological tests revealed normocytic non-regenerative anemia associated with hemolysis, thrombocytopenia, and elevated sideremia, ferritin, and transferrin saturation levels. Megaloblastic anemia was confirmed with undetectable blood vitamin B12 and typical cytological findings like hyper-segmented neutrophils in blood and megaloblasts in bone marrow. The patient received vitamin B12 supplementation. At 3 months, biological parameters reached normal values. Hepcidin kinetics from diagnosis to 3 months inversely correlated with those of erythroferrone and growth differentiation factor-15.

Conclusions
This case suggests that iron-overload mechanisms of dyserythropoietic anemias may apply to megaloblastic anemias.
Conservation laws and exact solutions of the $(3+1)$-dimensional Jimbo–Miwa equation
Authors: Manafian Jalil , Alimirzaluo Elnaz , Nadjafikhah Mehdi 
Published: September 2021

AbstractIn this article, by using the Herman–Pole technique the conservation laws of the $(3+1)-$
(
3
+
1
)
−
Jimbo–Miwa equation are obtained, and then by using the Lie symmetry analysis all of the geometric vector fields of this equation are given. Also, the non-classical symmetries of the Jimbo–Miwa equation have been determined by applying nonclassical schemes. Eventually, the ansatz solutions of the Jimbo–Miwa equations utilizing the tanh technique have been offered.
A generalized multivariable Newton method
Authors: Burachik Regina S. , Caldwell Bethany I. , Kaya C. Yalçın 
Published: September 2021

AbstractIt is well known that the Newton method may not converge when the initial guess does not belong to a specific quadratic convergence region. We propose a family of new variants of the Newton method with the potential advantage of having a larger convergence region as well as more desirable properties near a solution. We prove quadratic convergence of the new family, and provide specific bounds for the asymptotic error constant. We illustrate the advantages of the new methods by means of test problems, including two and six variable polynomial systems, as well as a challenging signal processing example. We present a numerical experimental methodology which uses a large number of randomized initial guesses for a number of methods from the new family, in turn providing advice as to which of the methods employed is preferable to use in a particular search domain.
Influence of sugammadex versus neostigmine for neuromuscular block reversal on the incidence of postoperative pulmonary complications: a meta-analysis of randomized controlled trials
Authors: Wang Jia-Feng , Zhao Zhen-Zhen , Jiang Zheng-Yu , Liu Hui-Xing , Deng Xiao-Ming 
Published: September 2021

Abstract
Background
The influence of sugammadex for reversal of neuromuscular block (NMB) on postoperative pulmonary complications (PPCs), compared with neostigmine, remains to be determined. We performed a meta-analysis of randomized controlled trials (RCTs) to compare the incidence of PPCs between patients who received sugammadex versus neostigmine.

Methods
Relevant studies were obtained by searching the PubMed, Embase, and Cochrane Library databases. A random effects model incorporating the potential heterogeneity was used to pool the results.

Results
Fourteen RCTs including 1478 adult patients who underwent surgeries with general anesthesia were included, and of these, 753 received sugammadex and 725 received neostigmine for reversal of NMB. The pooled results showed that sugammadex was associated with a lower risk of overall PPCs compared to neostigmine (odds ratio [OR]: 0.62, 95% confidence interval [CI]: 0.43–0.89, p = 0.01; I2 = 0%). This finding remained consistent after exclusion of two studies with potential overlapping events (OR: 0.58, 95% CI: 0.36–0.96, p = 0.03; I2=9%). Stratified analyses according to the categories of PPCs showed that sugammadex was associated with a significantly lower risk of postoperative respiratory failure (OR: 0.60, 95% CI: 0.38–0.97, p = 0.04; I2 = 0%) but not of postoperative pulmonary infection (OR: 0.79, p = 0.71), atelectasis (OR: 0.78, p = 0.33), or pneumothorax (OR: 0.87, p = 0.79).

Conclusions
Compared with neostigmine, the use of sugammadex for reversal of NMB was associated with a lower risk of PPCs, mainly due to a lower incidence of postoperative respiratory failure with the use of sugammadex.
The relationship sabotage scale: an evaluation of factor analyses and constructive validity
Authors: Peel Raquel , Caltabiano Nerina 
Published: September 2021

AbstractBackgroundSome individuals are no longer entering romantic relationships, others move through relationships too quickly searching for “the one” and making quick assessments of their romantic partners, while others stay in their relationships but “check out” or do not work on their issues. These are conclusions from two studies: (1) an interview with psychologists who specialise in relationship therapy, and (2) an analysis of individuals’ lived experiences of relationships. The concept of relationship sabotage can explain these phenomena. However, presently, there is no instrument to conceptualise and empirically measure how people continue to employ self-defeating attitudes and behaviors in (and out) of relationships to impede success, or withdraw effort, and justify failure.Methods and ResultsA series of three studies (involving a total of 1365 English speaking individuals of diverse gender orientation, sexual orientation, and cultural background, with relationship sabotage experience) were conceptualized for the current project to fill the need for scale development and to build empirical evidence on the topic of self-sabotage in romantic relationships. The scale was developed over two studies using exploratory factor analysis and one-congeneric model analyses. The third study, using confirmatory factor analysis, confirmed the final structure for the Relationship Sabotage Scale (RSS), which contains 12 items and three factors: defensiveness, trust difficulty, and lack of relationship skills. Constructive validity analyses were also conducted.ConclusionThe RSS is a brief scale that provides conclusive information about individual patterns in relationships. Findings using this scale can offer explanations regarding the reasons that individuals engage in destructive behaviours from one relationship to the next. Investigations should continue to test a model for sabotage in romantic relationships using the developed scale and other factors such as relationship differences and insecure attachment. More specifically, this measure can be used to understand mediator constructs of relational outcomes within the attachment framework to explain relationship dissolution and work towards relationship maintenance.