bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSX-B-17 Appeasing substance application at weaning enhanced growth and temperament of bos indicus-influenced weaned calves in grazing system
Authors: Viera Douglas G , Ranches Juliana , Cappellozza Bruno I , Fernandes Henrique J , D’Oliveira Marcella , Rocha Raizza , Valério Luan , Curcio Uriel , Franco Gumercindo L 
Published: October 2021

Abstract
The objective of this study was to evaluate the effects of a synthetic analogue of the bovine appeasing pheromone (i.e. bovine appeasing substance; BAS) on growth and temperament of weaned calves grazing Capim-Marandú (Urochloa brizantha cv Marandú). At weaning (d 0), 86 calves (47 steers and 39 heifers; Aberdeen Angus ′ Nelore; 8 ± 1 mo) were stratified by body weight (197.9 ± 24.9 kg) and randomly assigned to receive a single dose of BAS (n = 43; SecureCattle; Nutricorp, Araras, SP, Brazil) or saline (CON; n = 43; saline 0.9% NaCl). Treatments (5 ml) were topically applied to the nuchal skin area of each animal on d0. Body weight was collected on d 0, 8, 15, 51 and 100. Chute score (1 to 5; 1 = calm, no movement; 5 = violent and continuous struggling) and chute entrance and exit scores (1 to 3; 1 = slow; 3 = fast) were collected on d 3, 8, 15, 51 and 100. Scores were averaged across 3 trained technicians. Data were analyzed using the MIXED procedure of SAS. Calves assigned to BAS treatment had greater (P &lt; 0.01) average daily gain (ADG) from d 8 to 15 (0.158 and -0.284 kg/d ± 0.07). Calves assigned to BAS treatment tended (P = 0.10) to have lower chute entrance score on d 8 and 51 and had lower (P = 0.02) chute exit score on d 8 than calves assigned to CON treatment. Additionally, calves assigned to BAS treatment tended (P = 0.07) to have lower chute score on d 8 when compared to calves assigned to CON treatment. The application of BAS at weaning improved calf ADG and improved calf temperament as observed by chute score and chute entrance and exit scores, suggesting that BAS application has calming effects.
PSXVI-20 Real-time localization system for dairy cattle in a commercial facility: Evaluation of dynamic positioning ability
Authors: Rice Emily M , Costa Joao H C 
Published: October 2021

Abstract
Real-time location tracking of dairy cattle on commercial facilities enables producers to quickly locate and tend to cows and provides insight into health status and animal welfare. The effectiveness of these systems depends on accurate and reliable reporting of information. Therefore, this experiment’s objective was to assess the dynamic positioning abilities of a commercially available real-time location ear tag system (SmartBow; Zoetis, Parsippany, NJ) for use in group-housed dairy cattle. Using 2 pens of a freestall barn, cameras (n = 18) were installed on barn fixtures and positioned to photograph cow usage of feedbunk (n = 4), stall (n = 10), or waterer (n = 4). Photographs were captured automatically at 1-min intervals for 85 hours, consecutively. Laser measurements determined X and Y coordinates for reference point locations respective to camera. In dynamic position assessments, variations in ear tag proximity to measured reference points are to be expected. One trained observer reviewed photographs, saving only those depicting cows with visible farm-assigned ID. Data points were only defined if farm-assigned ID was associated with an active technology ear tag. For data analyses, technology-reported X and Y coordinates of data points were compared to reference point locations. Of all data points (n = 114,633), mean distance error was 3.82 m (SD= 6.73 m). Median distance error was 2.44 m (SD= 6.73; Q1= 1.41 m; Q3=5.13 m). With removal of outliers (99th percentile), data (n = 113,487) had a mean distance error of 3.82 m (min= 0.01 m; max= 33.80 m) and a median of 2.41 m (SD= 2.78 m; Q1= 1.40 m; Q3= 4.95 m). Using distance error, mean percentages of data points that fell within specified radii of circles centered about reference point locations were calculated (Table 1). These results suggest that this ear tag technology can accurately report the dynamic positions of dairy cattle moving freely in a commercial freestall barn environment.
PSI-B-28 Breed effect on weight gain of broilers
Authors: Oladipo Sodiq , Kareem-Ibrahim Khadeejah , Abanikannda Olatunji T 
Published: October 2021

Abstract
Poultry production as an aspect of livestock production is important to the biological needs, economic and social development of the people in any nation. Broiler birds are specifically bred for rapid growth to attain mature body size within 7–10 weeks depending on the strain, sex and management. This study was carried out in the humid tropics of Southwestern Nigeria. The experimental units were derived from four commercial broiler breeds namely Arbor Acre, Cobb, Marshall and Ross. Each breed had 76 chicks totaling 304 across the four breeds. On arrival, each chick was tagged by breed and identification number, and the initial weight of the chicks were recorded. Each of the breeds were thereafter randomly selected and randomly assigned to four experimental plots as replicates of the same treatment. The broiler birds were reared for 10 weeks and their weight taken and recorded at weekly intervals. All statistical analyses were conducted using boxplot, descriptive statistic and general linear models of Minitab® 16. After exploratory analysis to check for normality and outliers, a total of 217 birds were used in the final analyses. Except for the Marshall breed that had a highly significantly (P &lt; 0.01) lower weight at hatching, other breeds had fairly similar weight (Table 1). However, there was no statistical (P &gt; 0.05) difference in mean initial weight across the four replicates. While breed alone accounted for 37.81% of the total variation in initial weight, it only accounted for 30.07% of the difference in weight gain. The effect of breed on weight gain was only significant (P &lt; 0.01) in Marshall, while the other three breeds were not statistically different (P &gt;0.05). It can be deduced from this study that performance in terms of weight gain of most of the commercially available breeds in Nigeria are similar with the exception of Marshall.
PSVI-19 Evaluation of birth weight, weaning weight and average daily weight gain of Holstein female calves carrying the SLICK1 allele of the Prolactin Receptor (PRLR) gene
Authors: Carmickle Allie T , Zamaroni Madison R , Pereira Jessica , Hansen Peter J , Denicol Anna C 
Published: October 2021

Abstract
Objectives of this study were to evaluate birth weight (BW), weaning weight (WW), and average daily weight gain (ADG) of female calves carrying the SLICK1 allele, i.e., the slick gene. Holstein cows in four dairy farms located in central California were inseminated with semen from two heterozygous slick Holstein sires to produce slick and non-slick calves. Calves were born during the cool season (November 2019-March 2020). BW was recorded for 125 calves in four farms within 24 h of birth; WW was recorded for 92 calves in three farms within one week of weaning. Weaning age was recorded at day of weighing. ADG was calculated as the weight difference between birth and weaning divided by days of age at weaning. Only female calves were used in the analysis. Statistical analyses were performed using the GLIMMIX procedure of SAS version 9.4. The model for BW included the effects of genotype, farm, sire, and the interaction between genotype and farm. The model for WW and ADG included the effects of BW, genotype, farm, weaning age, sire, and interaction between genotype and farm. Significant effects were considered as those with P &lt; 0.05. There was no difference in BW or WW between genotypes (BW: slick=40.1 ± 0.7 vs non-slick=39.7 ± 0.6 kg; P = 0.7 and WW: slick=82.5 ± 2.8 vs non-slick=80.9 ± 2.8 kg; P = 0.6). The mean age at weaning was 64.8 ± 0.4 and was similar for both genotype groups (P = 0.8). As a result, ADG was similar between genotypes (P = 0.7). There was a main effect of farm on BW, WW and ADG. Results indicate that slick heifer calves born in the cool season perform similarly to non-slick calves in regards to birth weight, average daily weight gain, and weaning weight. Funding provided by the Holstein Association USA Research Program and L.E. “Red” Larson Endowment.
Retracted: PSVIII-10 Genome-wide CNV analysis unravels a deletion associated with parasite resistance in Florida native sheep
Authors: Diehl Brittany N , Pech-Cervantes Andres A , Terrill Thomas H , Ogunade Ibukun M , Rae Owen , Estrada-Reyes Zaira M 
Published: October 2021

Abstract
This poster abstract has been retracted at the request of the conference organizers, who were notified by the first author that they had been included without their knowledge and without participation in the research. The co-presenters have been unresponsive to attempts to contact them for explanation.
PSXI-10 How to use ultrasonic cutter on frozen semen for multiple embryo production
Authors: Kim Sung Woo , Lee Jae-Yeong , Kim Chan-Lan , Hwang In-Sul , Ko Yeoung-Gyu , Lee Sung-Soo 
Published: October 2021

Abstract
The objective of the present study was to establish conditions for using technology that can potentially enhance the efficiency of bovine embryos derived from in vitro fertilization (IVF) with frozen semen. Frozen semen from selected bulls can be stored indefinitely in liquid nitrogen as genetic resources; however, these resources are considered consumable because they cannot be regenerated. Therefore, to optimize the utilization of frozen semen, as many oocytes as possible should be fertilized with one straw. However, a sufficient number of prepared oocytes might not be available for one experiment, which can limit the use of the total spermatozoa population. Thus, an economical method for producing embryos needs to be established by optimizing technology for transplantable embryos. In this study, the utilization of frozen semen was increased by dividing the straw with an ultrasonic cutter. The post-thaw survival rate of uncut straws from Korean Proven Bulls did not differ from that of half cuttings. When ultrasonic cutting was applied to frozen semen, spermatozoa could be prepared for IVF trials at least four times, and blastocysts were produced. Therefore, cutting frozen semen with an ultrasonic cutter represents a potentially useful tool to expand genetic resources from excellent breeding stocks. This approach could also be valuable in the field of IVF of endangered species or rare breeds for their preservation, as well as in ovum pick-up (OPU) techniques.
PSXII-11 Parameters for yearling wool production, wool quality and liveweight traits in mutton-wool breed of sheep with a different kinship ratio
Authors: Sabrekova Valentina , Korenyuga Maxim , Timoshenko Julia , Feyzullaev Feyzullah 
Published: October 2021

Abstract
This study aimed to determine the effect of crossbreeding on wool production for sheep of different kinship ratios. Purebred (Volgograd) and crossbred (F3, Volgograd x ¼ North Caucasian) sheep were used. The birth liveweights (n = 100) of tups were similar (3.71 kg and 3.69 kg) whereas crossbred ewes were heavier than purebreds (3.45 kg vs 3.3 kg). At 2.5 months (n = 97), crossbred tups outweighed purebreds by 6.94% (22.17 kg and 20.73 kg (p ≤ 0.05); ewes’ liveweights were similar (19.98 kg and 19.88 kg). Crossbred tups’ weaning weights (n = 70) exceeded purebred tups by 20.85% (32.33 kg and 26.75kg, (p ≤ 0.01); crossbred ewes outweighed purebreds by 7.38% (27.20 kg and 25.33 kg). Crossbred tup yearlings (n = 67) outweighed purebreds (52.10 and 48.90 kg) (P ≤ 0.01) whereas ewe yearlings were similar (37.30 kg vs. 36.70 kg). However, fleece weights (n = 30) of crossbred tups were heavier than of purebreds. Greasy fleece weight of crossbred tups was 6.72 kg and 6.14 kg (P ≤ 0.05). Clean fleece weight of crossbred tups outweighed purebred by 13.73% (3.56 kg and 3.13 kg, P ≤ 0.05). Greasy fleece weight of crossbred ewes was 3.95 kg and 3.57 kg (P ≤ 0.05). Clean fleece weight of crossbred tups outweighed purebred by 15.34% (2.18 kg and 1.89 kg, P ≤ 0.05). Wool yield of crossbred and purebred tups were 53 and 51%, ewes were 55 and 53%. Staple lengths of crossbred and purebred tups were 12.53 cm and 10.51 cm (P ≤ 0.001), crossbred and purebred ewes were 11.48 cm and 9.56 cm (P ≤ 0.01). This study indicates there was no negative effect of an admixture of new blood of North Caucasian breed on wool productivity of F3. These results can help improve meat and wool production.
PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep
Authors: Shakhin Alexey V , Dotsev Arsen V , Deniskova Tatiana E , Brem Gottfried , Zinovieva Natalia A 
Published: October 2021

Abstract
Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.
PSXII-3 Identification of key metabolic mediators during dietary tryptophan supplementation in low-birth-weight pigs by RNA sequencing
Authors: Xiao Ping , Goodarzi Parniyan , Habibi Mohammad , Roberts Kennedy , Sutton Julia , Shili Cedrick , Pezeshki Adel , Hagen Darren 
Published: October 2021

Abstract
Low birth weight (LBW) is associated with the development of metabolic syndrome, diabetes mellitus and insulin resistance. While tryptophan (Trp), one of essential amino acids supplied by diet, plays an essential role in fetal growth and development, the effects of Trp supplementation on metabolic processes of postnatal LBW individuals remain unclear. The objective of this study was to identify differentially expressed (DE) genes and altered biological processes in liver of LBW and normal birth weight (NBW) piglets supplemented with Trp. RNA was isolated from liver tissues of three weeks old piglets supplemented with Trp (NBW-0% and LBW-0, 0.4, and 0.8%) and was used for RNA sequencing (RNAseq). There were 100, 191 and 39 DE genes in NBW (N0) and LBW tryptophan 0.4%, 0.8% (L4 and L8) when compared to LBW without Trp supplementation (L0). To determine whether Trp supplementation can resume metabolic regulation-related gene expression to N0 level, DE genes from N0 vs. L0 were clustered into 3 groups based on co-expression trends and clusters were enriched for genes associated with lipid catabolic process, circadian regulation of gene expression and fatty acid response. Further, eight hub genes (PID1, PAFAH2, MAP3K15, ANKRD44, CYP2J34, N4BP2L1, RUSC1 and SALL1) identified in co-expression networks based on Pearson correlation coefficient had strong co-expression coefficients (|r| &gt; 0.9) with each other. In particular, PID1 was significantly associated with many neurological, metabolic, environmental and cardiovascular traits based on phenome-wide association analysis (Phe-WAS). In summary, our study provides novel insights into the molecular mechanism underlying LBW metabolic changes with Trp supplementation in porcine liver tissue and highlights that LBW metabolism restoration may be regulated by genes participating in fatty acid response and cardiovascular diseases.
PSXIV-27 Simulation controlling inbreeding for selection experiments on woody breast meat in broiler
Authors: JEON Hye Rin , SHIN Seungjun , OH Sang-Hyon 
Published: October 2021

Abstract
The purpose of this study was to control inbreeding for selection experiments on woody breast (WB) meat in broiler. The simulation was designed to figure out which mating plan would show proper breeding values while optimizing inbreeding assuming that a selection study would be done on WB in broilers starting with 500 males and 500 females as a foundational population. The simulated selections were based on Optimum Genetic Contribution theory (OGC) under different conditions over 10 generations, which uses relationships among individuals as weighting factors. It is selecting individuals by weighting estimated breeding values with average relationships among individuals. From the 2nd generation, various selection plans were considered in each sex, which were top 10, 20, 50 and 100 males selected, and top 100 and 200 females selected every generation. Each female bird was assumed to have 10 eggs. The algorithm is as follows: 1) Identify the individual having the best EBV; 2) Calculate average relationships between selected and candidates; 3) Select the individual having the best EBV adjusted for average relationships using the weighting factor k; 4) Repeat process until the number of individuals selected equals number required. Three different weighting values (k=0, 1, 2) were used, which made a total 24 different conditions compared (4×2×3). Additive genetic variance of breast meat was 1.134. Mendelian sampling terms were also considered when the breeding values were generated. Results showed that higher k value (k=2) controlled effectively inbreeding and maintained consistent increases in selection response. Differences in breeding values among selection plans with OGC algorithm and by EBV only was 4% on average; however, average rate of inbreeding (0.1) was controlled by 27% after 10 generations. These results indicate that the OGC algorithm can be used effectively in a short-term selection program with the relatively smaller number of populations.
PSXV-10 Study of allelic pattern of PLAG1 gene in the historical and modern populations of two oldest Russian dairy cattle breeds
Authors: Abdelmanova Alexandra S , Kharzinova Veronika R , Dotsev Arsen V , Sermyagin Alexander A , Boronetskaya Oxana , Chinarov Roman , Brem Gottfried , Zinovieva Natalia A 
Published: October 2021

Abstract
Increasing animal stature is one of the goals of modern breeding programs for many dairy cattle breeds, because stature related to higher milk yield. The PLAG1 gene was shown to be a strong candidate responsible for stature in different cattle breeds. The polymorphic SNP BovineHD1400007259, located within PLAG1 gene, is considered as a causal mutation responsible for stature. The aim of our work was to evaluate the effect of long selection for the increased body height on the alterations of the allele’s frequencies of the PLAG1 gene in the historical and modern populations of the Russian Yaroslavl and Kholmogor dairy cattle breeds. The historical specimens of Yaroslavl (n = 22) and Kholmogor (n = 12) cattle dated by the first quarter of the 20th century were derived from the craniological collection of the E.F. Liskun Museum for Animal Husbandry. The modern representatives of Yaroslavl (n = 31) and Kholmogor (n = 25) breeds were used for comparison. All works with historical samples were performed in dedicated facility of the L.K. Ernst Research Center for Animal Husbandry. The samples were genotyped using high-density DNA arrays (Illumina Inc., USA). The historical DNA was treated by USER enzyme before genotyping to avoid the misincoporated nucleotides occurred due to postmortem DNA damage. We observed significant differences in allele frequencies of PLAG1 genes between historical and modern populations of both breeds. The frequencies of G allele, which is associated with higher stature, were increased from 0.114 in historical Yaroslavl cattle and from 0.167 in historical Kholmogor cattle to 0.633 and 0.860 in the modern breeds’ representatives, respectively. Our data suggest that PLAG1 gene was affected by artificial selection in studied cattle breeds. The research results will be useful for elucidation of the history of these two oldest Russian dairy cattle breeds. The study was funded by the RSF No. 21-66-00007.
PSXV-7 The mixture of herbal extracts that change the microbiome of the cattle rumen
Authors: Tuzikov Roman , Nurzhanov Baer , Rysaeff Albert , Duskaev Galimzhan , Rakhmatullin Shamil , Kvan Olga , Shejjda Elena 
Published: October 2021

Abstract
Today, the search for new antibacterial natural agents based on plant raw materials is relevant. These raw materials have promising secondary metabolites that reduce the formation of methane in the ruminant scar. The aim of this study was to evaluate the effect of the following extracts: oak bark+birch leaves (I group) (250 ml/head per day), oak bark + St. John’s wort grass (250 ml / head per day; II group) and birch leaves + St. John’s wort grass (III group; 250 ml/head per day) on the rumen microbiota of large cattle, against the background of control (C). Extracts added to the water of calves with rumen fistula (dairy breed, age 12 months, diet -70 % of hay, 30 % of grain feed, duration is 14 days). The microflora analyzed using high efficiency 16S rRNA (Illumina) gene sequencing. Plant extracts prepared by purification, grinding (1–2 mm) and extraction in a water bath (for 30 min, 70 ° C) and filtration. According to the study results, the inclusion of extracts led to increase of the Bacteroidetes phylum for 1.11–3.69 % in the practical groups in comparison with C. A decrease was obtained for Firmicutes for 2.06–3.55 % (p 0.05) and Proteobacteria. In the I group bacteria of the class Bacteroides increased by 0.9–5.64% and Candidatus Saccharibacteria increased by 0.25% (P = 0.05). In the III group the value increased by 0.64 % (P = 0.05). Compared to C at the level of the family Prevotellaceae dominated in all experimental groups by 1.04–9.11 % (P ≤ 0.05), in group III Bacteroidaceae dominated by 0.27 % and Rikenellaceae by 0.14 %. This contributes to the regulation of opportunistic microorganisms without harmful effects on the rumen microbiota. This research was performed with financial support from the project 0761-2019-0005.