bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome after hematopoietic stem cell transplantation in patients with extranodal natural killer/T‐Cell lymphoma, nasal type: A French study from the Société Francophone de Greffe de Moelle et de Thérapie Cellulaire (SFGM‐TC)
Authors: Philippe Walter Laure , Couronné Lucile , Jais Jean‐Philippe , Nguyen Phong‐Dinh , Blaise Didier , Pigneux Arnaud , Socié Gérard , Thieblemont Catherine , Bachy Emmanuel , Fegueux Nathalie , Chevallier Patrice , Mohty Mohamad , Yakoub‐Agha Ibrahim , Peffault de Latour Regis , Jaccard Arnaud , Hermine Olivier ,   for the Société Française de Greffe de Moelle et de Thérapie Cellulaire (SFGM‐TC)
Published: April 2021

AbstractWe evaluated the outcome of 65 French patients with Extranodal NK/T‐cell lymphoma, nasal type (ENKTL) undergoing hematopoietic stem cell transplantation (HSCT) (19 allogeneic and 46 autologous). Fifty‐four patients (83%), most of which receiving L‐asparaginase (L‐aspa) containing regimens (81%), achieved complete or partial response at time of HCST. After a median follow‐up of 79.9 months, 4‐years progression‐free survival (PFS) and overall survival (OS) were similar in both autologous and allogeneic groups (PFS: 34% vs. 26%, p = .12 and OS: 52% vs. 53%, p = .74). Response status at HSCT was the major independent prognostic factor on survival (OS: HR: 4.013 [1.137; 14.16], p = .031 and PFS: HR: 5.231 [1.625; 16.838], p = .006). As compared to control patients receiving chemotherapy and/or radiotherapy containing regimens only, upfront HSCT did not improve the outcome of responder patients, including those treated by L‐aspa. However, it tends to provide survival benefit for relapsed patients with initial high‐risk clinical features who achieved second remission. Whereas the place of HSCT in upfront therapy has still to be clarified, these data confirm that HSCT should be considered for consolidation in selected patients with relapsed ENKTL. Based on a large non Asian ENKTL cohort since the L‐aspa era, this study provides some insight into the survival patterns of ENKTL patients with HSCT in the Western hemisphere and may give future direction for the next clinical trial design.
Sequence of proteome profiles in preclinical and symptomatic Alzheimer's disease
Authors: Li Xiaohang , Tsolis Konstantinos C. , Koper Marta J. , Ronisz Alicja , Ospitalieri Simona , von Arnim Christine A.F. , Vandenberghe Rik , Tousseyn Thomas , Scheuerle Angelika , Economou Anastassios , Carpentier Sebastien , Otto Markus , Thal Dietmar Rudolf 
Published: April 2021

AbstractProteome profile changes in Alzheimer's disease (AD) brains have been reported. However, it is unclear whether they represent a continuous process, or whether there is a sequential involvement of distinct proteins. To address this question, we used mass spectrometry. We analyzed soluble, dispersible, sodium dodecyl sulfate, and formic acid fractions of neocortex homogenates (mainly Brodmann area 17‐19) from 18 pathologically diagnosed preclinical AD, 17 symptomatic AD, and 18 cases without signs of neurodegeneration. By doing so, we identified four groups of AD‐related proteins being changed in levels in preclinical and symptomatic AD cases: early‐responding, late‐responding, gradually‐changing, and fraction‐shifting proteins. Gene ontology analysis of these proteins and all known AD‐risk/causative genes identified vesicle endocytosis and the secretory pathway‐related processes as an early‐involved AD component. In conclusion, our findings suggest that subtle changes involving the secretory pathway and endocytosis precede severe proteome changes in symptomatic AD as part of the preclinical phase of AD. The respective early‐responding proteins may also contribute to synaptic vesicle cycle alterations in symptomatic AD.
Peripartum management of a parturient with Klippel‐Trénaunay syndrome
Authors: Moxon H. M. , Kennedy H. S. 
Published: April 2021

SummaryKlippel‐Trénaunay syndrome is a rare congenital disorder affecting the vascular and lymphatic systems. The clinical presentation can vary widely, but the syndrome is broadly characterised by capillary, venous and lymphatic malformations as well as limb hypertrophy. We present the case of a 35‐year‐old parturient who underwent an emergency caesarean section for suspected fetal distress, and describe the anaesthetic management during the peripartum period. Only a small number of similar cases have been described, and the multisystem nature of the condition presents several challenges to both the obstetric and anaesthetic management. The major points of concern to the anaesthetist are haematological, with a tendency to both abnormal bleeding and clotting disorders, compounded by vascular malformations which may present anywhere in the body including the epidural space and airway. Other considerations relate to limb hypertrophy and spinal abnormalities, as well as pulmonary and ocular sequelae and chronic pain. Strategies for safe patient management include early multidisciplinary involvement, and assessment of the presence and extent of any vascular anomalies with advanced imaging techniques. The risk of significant blood loss can be mitigated with antifibrinolytic and uterotonic medication as well as cell salvage, with treatment carefully balanced against the concurrent risk of thrombosis.
Green synthesis of NiONPs using Trigonella subenervis extract and its applications as a highly efficient electrochemical sensor, catalyst, and antibacterial agent
Authors: Mahdavi Behnam , Paydarfard Sogand , Rezaei‐Seresht Esmail , Baghayeri Mehdi , Nodehi Marzieh 
Published: April 2021

In recent years, the biosynthesized of metallic nanoparticles has been rapidly growing due to their environmentally synthesis and widespread applications in science and industry. In this study, we have reported the green synthesis of NiONPs using an aqueous extract of Trigonella subenervis (NiONPs@TS). The nanoparticles were characterized by Fourier transform infrared spectroscopy (FT‐IR), X‐ray diffraction (XRD), field emission scanning electron microscopy (FE‐SEM), and energy‐dispersive X‐ray spectroscopy (EDS). To find various applications for NiONPs@TS, the nanoparticles (NPs) were used as a catalyst for synthesizing some benzimidazole derivatives. The electrochemical behavior and antibacterial activity of NiONPs@TS were also evaluated. FE‐SEM images exhibited a uniform spherical morphology in size of 28.21 nm for the biosynthesized nanoparticles. According to the XRD analysis, 26.43 nm was measured for NiONPs@TS crystal size. Benzimidazole derivatives were synthesized using NiONPs@TS as the catalyst with a yield of 69–92%. NiONPs@TS showed a significant electrocatalytic activity to glucose oxidation. The linear response range and detection limit were found to be 10–200 and 3.2 μM, respectively. Furthermore, NiONPs@TS exhibited an acceptable antibacterial activity in various assays, including well diffusion, disk diffusion, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC). The best antibacterial activity was obtained against Bacillus subtilis with MIC of 4.0 ± 0.0 μg/ml and MBC of 6.7 ± 2.3 μg/ml. The obtained results showed the ability of NiONPs@TS in different fields such as catalytic, electrochemical sensing, and antibacterial activity, which can be contributed to the presence of biomolecules in T. subenervis extract.
Synergistic improvement of mechanical and thermal properties in epoxy composites via polyimide microspheres
Authors: Xu Xiaoqing , Hu Dechao , Ma Wenshi 
Published: April 2021

AbstractAchieving synergetic improvements of mechanical strength, toughness, and thermal stability of epoxy resin has been a crucial but very challenging issue. Herein, to explore a new solution for circumventing this issue, polyimide microspheres were successfully prepared through the inverse nonaqueous emulsion process, and the structure, size distribution and morphologies of polyimide (PI) microspheres were comprehensively investigated. Then the PI microspheres were incorporated in epoxy resin matrix to systematically investigate the mechanical and thermal properties of obtained epoxy/PI microspheres composites. It was found that the PI microspheres can not only enhance the mechanical strength of epoxy resin, but also significantly improve the toughness. Specially, the epoxy‐based composites containing 3 wt% PI microspheres exhibit a 47% increase in tensile strength, while theGICand Charpy impact strength increase by 106% and 200%, respectively. The toughing mechanism of epoxy/PI microspheres composites was discussed. Moreover, the PI microspheres can also endow the epoxy resin with excellent thermal stability and heat resistance. Thus, this work may open a new opportunity to synergistically enhance the mechanical and thermal properties of epoxy‐based composites and may also give some valuable inspiration for the rational design of other high‐performance thermosetting composites.
Performance tuning of glass fiber/epoxy composites through interfacial modification upon integrating with dendrimer functionalized graphene oxide
Authors: Jamshaid Fahd , Khan Rafi Ullah , Islam Atif 
Published: April 2021

AbstractIn this study, glass fiber/epoxy composites were interfacially tailored by introducing polyamidoamine (PAM) dendrimer functionalized graphene oxide (GO) into epoxy matrix. Two different composites each containing varying loading fraction (0.5, 1.0, and 1.5 wt%) of GO and GO‐PAM were fabricated via hot press processing. Composites were evaluated for interlaminar shear strength (ILSS), dynamic mechanical properties and thermal conductivity. The inclusion of 1.5 wt% GO‐PAM resulted ~57.3%, ~42.7%, and ~54% enhancement in ILSS, storage modulus and thermal conductivity, respectively. Almost, ~71% reduction in coefficient of thermal expansion was also observed at same GO‐PAM loading. Moreover, higher glass transition temperature was observed with GO‐PAM addition. GO‐PAM substantially improved fiber/matrix interfacial adhesion, which was witnessed through scanning electron microscopy. The enhanced thermo‐mechanical performance was attributed to interfacial covalent interactions engendered by ring opening reaction between epoxy and amine moieties of PAM dendrimers. These multiscale composites with extraordinary functional properties can outperform conventional counterparts with improved reliability and performance.
Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organoids reveals faster commitment under dynamic conditions
Authors: Silva Teresa P. , Sousa‐Luís Rui , Fernandes Tiago G. , Bekman Evguenia P. , Rodrigues Carlos A. V. , Vaz Sandra H. , Moreira Leonilde M. , Hashimura Yas , Jung Sunghoon , Lee Brian , Carmo‐Fonseca Maria , Cabral Joaquim M. S. 
Published: April 2021

AbstractHuman‐induced pluripotent stem cells (iPSCs) have great potential for disease modeling. However, generating iPSC‐derived models to study brain diseases remains a challenge. In particular, the ability to recapitulate cerebellar development in vitro is still limited. We presented a reproducible and scalable production of cerebellar organoids by using the novel single‐use Vertical‐Wheel bioreactors, in which functional cerebellar neurons were obtained. Here, we evaluate the global gene expression profiles by RNA sequencing (RNA‐seq) across cerebellar differentiation, demonstrating a faster cerebellar commitment in this novel dynamic differentiation protocol. Furthermore, transcriptomic profiles suggest a significant enrichment of extracellular matrix (ECM) in dynamic‐derived cerebellar organoids, which can better mimic the neural microenvironment and support a consistent neuronal network. Thus, an efficient generation of organoids with cerebellar identity was achieved for the first time in a continuous process using a dynamic system without the need of organoids encapsulation in ECM‐based hydrogels, allowing the possibility of large‐scale production and application in high‐throughput processes. The presence of factors that favors angiogenesis onset was also detected in dynamic conditions, which can enhance functional maturation of cerebellar organoids. We anticipate that large‐scale production of cerebellar organoids may help developing models for drug screening, toxicological tests, and studying pathological pathways involved in cerebellar degeneration.
A Novel D‐A‐D Photosensitizer for Efficient NIR Imaging and Photodynamic Therapy
Authors: Liu Yuhan , Chen MiaoMiao , Zhao Yanliang , Lv Shibo , Zheng Daoyuan , Liu Dapeng , Song Fengling 
Published: April 2021

AbstractPhotodynamic therapy (PDT) has attracted great interest in cancer theranostics owing to its minimal invasiveness and low side effect. In PDT, photosensitizers are indispensable components that generate cytotoxic reactive oxygen species (ROS). Tremendous efforts have been devoted to optimizing the photosensitizer with enhanced ROS efficiency. However, to improve the precision and controllability for PDT, developing NIR imaging‐guided photosensitizers are still urgent and challenging. Here, we have designed a novel photosensitizer 2Cz‐BTZ which integrated with intense NIR emission and photoinduced singlet oxygen 1O2 generation capabilities. Moreover, after loading the photosensitizers 2Cz‐BTZ into biocompatible amphiphilic polymers F127, the formed 2Cz‐BTZ@F127 nanoparticles (NPs) exhibited good photoinduced therapy as well as long‐term in vivo imaging capabilities. Under these merits, the 2Cz‐BTZ@F127 NPs showed NIR imaging‐guided PDT, which paves a promising way for spatiotemporally precise tumor theranostics.
Saccharified Uranyl Ions: Self‐Assembly of UO22+ into Trinuclear Anionic Complexes by the Coordination of Glucosamine‐Derived Schiff Bases
Authors: Schaper Gerrit , Wenzel Marco , Hennersdorf Felix , Lindoy Leonard F. , Weigand Jan J. 
Published: April 2021

AbstractThe reaction of UO2(OAc)2 ⋅ 2H2O with the biologically inspired ligand 2‐salicylidene glucosamine (H2L1) results in the formation of the anionic trinuclear uranyl complex [(UO2)3(μ3‐O)(L1)3]2− (12−), which was isolated in good yield as its Cs‐salt, [Cs]21. Recrystallization of [Cs]21 in the presence of 18‐crown‐6 led to formation of a neutral ion pair of type [M(18‐crown‐6)]21, which was also obtained for the alkali metal ions Rb+ and K+ (M=Cs, Rb, K). The related ligand, 2‐(2‐hydroxy‐1‐naphthylidene) glucosamine (H2L2) in a similar procedure with Cs+ gave the corresponding complex [Cs(18‐crown‐6)]2[(UO2)3(μ3‐O)(L2)3 ([Cs(18‐crown‐6)]22). From X‐ray investigations, the [(UO2)3O(Ln)3]2− anion (n=1, 2) in each complex is a discrete trinuclear uranyl species that coordinates to the alkali metal ion via three uranyl oxygen atoms. The coordination behavior of H2L1 and H2L2 towards UO22+ was investigated by NMR, UV/Vis spectroscopy and mass spectrometry, revealing the in situ formation of the 12− and 22−dianions in solution.
Applying Unconventional Spectroscopies to the Single‐Molecule Magnets, Co(PPh3)2X2(X=Cl, Br, I): Unveiling Magnetic Transitions and Spin‐Phonon Coupling
Authors: Bone Alexandria N. , Widener Chelsea N. , Moseley Duncan H. , Liu Zhiming , Lu Zhengguang , Cheng Yongqiang , Daemen Luke L. , Ozerov Mykhaylo , Telser Joshua , Thirunavukkuarasu Komalavalli , Smirnov Dmitry , Greer Samuel M. , Hill Stephen , Krzystek J. , Holldack Karsten , Aliabadi Azar , Schnegg Alexander , Dunbar Kim R. , Xue Zi‐Ling 
Published: April 2021

AbstractLarge separation of magnetic levels and slow relaxation in metal complexes are desirable properties of single‐molecule magnets (SMMs). Spin‐phonon coupling (interactions of magnetic levels with phonons) is ubiquitous, leading to magnetic relaxation and loss of memory in SMMs and quantum coherence in qubits. Direct observation of magnetic transitions and spin‐phonon coupling in molecules is challenging. We have found that far‐IR magnetic spectra (FIRMS) of Co(PPh3)2X2(Co‐X; X=Cl, Br, I) reveal rarely observed spin‐phonon coupling as avoided crossings between magnetic andu‐symmetry phonon transitions. Inelastic neutron scattering (INS) gives phonon spectra. Calculations using VASP and phonopy programs gave phonon symmetries and movies. Magnetic transitions among zero‐field split (ZFS) levels of theS=3/2 electronic ground state were probed by INS, high‐frequency and ‐field EPR (HFEPR), FIRMS, and frequency‐domain FT terahertz EPR (FD‐FT THz‐EPR), giving magnetic excitation spectra and determining ZFS parameters (D, E) andgvalues. Ligand‐field theory (LFT) was used to analyze earlier electronic absorption spectra and give calculated ZFS parameters matching those from the experiments. DFT calculations also gave spin densities inCo‐X, showing that the larger Co(II) spin density in a molecule, the larger its ZFS magnitude. The current work reveals dynamics of magnetic and phonon excitations in SMMs. Studies of such couplings in the future would help to understand how spin‐phonon coupling may lead to magnetic relaxation and develop guidance to control such coupling.
Organophotocatalytic Aerobic Oxygenation of Phenols in a Visible‐Light Continuous‐Flow Photoreactor
Authors: Wellauer Joël , Miladinov Dragan , Buchholz Thomas , Schütz Jan , Stemmler René T. , Medlock Jonathan A. , Bonrath Werner , Sparr Christof 
Published: April 2021

AbstractA mild photocatalytic phenol oxygenation enabled by a continuous‐flow photoreactor using visible light and pressurized air is described herein. Products for wide‐ranging applications, including the synthesis of vitamins, were obtained in high yields by precisely controlling principal process parameters. The reactor design permits low organophotocatalyst loadings to generate singlet oxygen. It is anticipated that the efficient aerobic phenol oxygenation to benzoquinones and p‐quinols contributes to sustainable synthesis.
FRugally Optimized DNA Octomer (FRODO) qPCR Measurement of HIV and SIV in Human and Nonhuman Primate Samples
Authors: Langner Charlotte A. , Brenchley Jason M. 
Published: April 2021

AbstractQuantitative polymerase chain reactions (qPCRs) are commonly employed to enumerate genes of interest among particular biological samples. Insertion of PCR amplicons into plasmid DNA is a mainstay for creation of known quantities of target sequences to standardize qPCRs. Typically, one amplicon is inserted into one plasmid construct, and the plasmid is then amplified, purified, serially diluted, and quantified to be used to enumerate target sequences in unknown samples. As qPCR is often used to detect multiple amplicons simultaneously, individual qPCR standards are often desired to normalize one to another. Here we report a single plasmid containing eight amplicons, which can be used to quantify several different strains of simian immunodeficiency virus and human immunodeficiency virus, cell number equivalents for humans and nonhuman primates, T cell receptor excision circles, and bacterial 16S DNA. This FRugally Optimized DNA Octomer (FRODO) plasmid was created and standardized to quantify all eight PCR amplicons. © 2021 US Government.Basic Protocol 1: Total genomic DNA extraction from primary cellsBasic Protocol 2: Quantitative PCR for viral, bacterial, and cell number equivalentsSupport Protocol: Purification, quantification, and storage of FRODO standard plasmid DNA
In Vivo and Ex Vivo Patient‐Derived Tumor Xenograft Models of Lymphoma for Drug Discovery
Authors: Cacciapuoti Maria Teresa , Cappelli Luca Vincenzo , Fiore Danilo , Toruno Pedro , Kayembe Clarisse , Tam Wayne , Inghirami Giorgio 
Published: April 2021

AbstractIn the hemato‐oncology field, remarkable scientific progress has been achieved, primarily propelled by the discovery of new technologies, improvement in genomics, and novel in vitro and in vivo models. The establishment of multiple cell line collections and the development of instrumental mouse models enhanced our ability to discover effective therapeutics. However, cancer models that faithfully mimic individual cancers are still imperfect. Patient‐derived tumor xenografts (PDTXs) have emerged as a powerful tool for identifying the mechanisms which drive tumorigenesis and for testing potential therapeutic interventions. The recognition that PDTXs can maintain many of the donor samples’ properties enabled the development of new strategies for discovering and implementing therapies. Described in this article are protocols for the generation and characterization of lymphoma PDTXs that may be used as the basis of shared procedures. Universal protocols will foster the model utilization, enable the integration of public and private repositories, and aid in the development of shared platforms. © 2021 Wiley Periodicals LLC.Basic Protocol 1: Tissue handling and cryopreservation of primary and PDTX samplesBasic Protocol 2: Performing tumor implant in immunocompromised mice PDTX modelsAlternate Protocol 1: Intra‐medullary femoral injectionAlternate Protocol 2: Intravenous injectionAlternate Protocol 3: Intraperitoneal injectionSupport Protocol 1: Phenotypical characterization of PDTXs by flow cytometrySupport Protocol 2: Biological and molecular characterization of PDTX tumors by PCR detection of IGK, IGH, and TCR rearrangementsBasic Protocol 3: Harvesting PDTX‐derived tumor cells for ex vivo experimentsBasic Protocol 4: In vivo testing of multiple compounds in a PDTX mouse model
Enhancing intergroup dialogues through art and art therapy
Authors: Potash Jordan S. , Gyimah‐Boadi Alberta 
Published: April 2021

AbstractIntergroup dialogue contributes to community development by providing structured and facilitated opportunities for narrative engagement and perspective taking that can foster social change. Both art and art therapy can be infused within intergroup dialogue to harness imagination and creativity in order to heighten reflection and promote emotional connections. An art therapy‐informed model enhanced self‐awareness, storytelling, and interpersonal interactions by helping participants discuss direct encounters of difference as well as specific events and people.