bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of acids, pepsin, bile acids, and trypsin on laryngopharyngeal reflux diseases: physiopathology and therapeutic targets
Authors: Li Yading , Xu Gaofan , Zhou Bingduo , Tang Yishuang , Liu Xiaowen , Wu Yue , Wang Yi , Kong Jing , Xu Tingting , He Cong , Zhu Shengliang , Wang Xiaosu , Zhang Jianning 
Published: December 2021

Abstract
Purpose
Laryngopharyngeal reflux disease (LPRD) is a general term for the reflux of gastroduodenal contents into the laryngopharynx, oropharynx and even the nasopharynx, causing a series of symptoms and signs. Currently, little is known regarding the physiopathology of LPRD, and proton pump inhibitors (PPIs) are the drugs of choice for treatment. Although acid reflux plays a critical role in LPRD, PPIs fail to relieve symptoms in up to 40% of patients with LPRD. The influence of other reflux substances on LPRD, including pepsin, bile acid, and trypsin, has received increasing attention. Clarification of the substances involved in LPRD is the basis for LPRD treatment.

Methods
A review of the effects of acids, pepsin, bile acids, and trypsin on laryngopharyngeal reflux diseases was conducted in PubMed.

Results
Different reflux substances have different effects on LPRD, which will cause various symptoms, inflammatory diseases and neoplastic diseases of the laryngopharynx. For LPRD caused by different reflux substances, 24-h multichannel intraluminal impedance combined with pH-metry (MII-pH), salivary pepsin, bile acid and other tests should be established so that different drugs and treatment courses can be used to provide patients with more personalized treatment plans.

Conclusion
This article summarizes the research progress of different reflux substances on the pathogenesis, detection index and treatment of LPRD and lays a theoretical foundation to develop target drugs and clinical diagnosis and treatment.
Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and FISH comparison
Authors: Brasil-Costa Igor , de Souza Carolina Rosal Teixeira , Costa Iran Barros , dos Santos Liann Filiphe Pereira , Paixão Luana César Ferraz , Polaro Alessandra Alves , Monteiro Talita Antonia Furtado , Burbano Rommel Mario Rodríguez 
Published: December 2021

AbstractEBV-associated gastric cancer accounts for about 10% of all gastric carcinomas worldwide. We aimed to verify the prevalence of EBV in gastric adenocarcinoma samples using FISH and qPCR and comparing the results obtained by both techniques. Gastric cancer samples from 191 cases were analyzed. The FISH assay was performed to detect small EBV RNAs (EBER1) and qPCR was performed to detect the EBV-EBNA-1 gene region. Cohen’s kappa index and the chi-square test were used to compare the methodologies and investigate correlations with the clinical-pathological data of the gastric adenocarcinoma patients. Most of the patients were men, and the average age was 60 years. The intestinal subtype cancer presented more aggressive stages with 90% of patients having a reactive FISH for EBV (EBV+), although the virus infection frequency in epithelial gastric tissue was only 1%. No positive association with clinicopathological features and EBV+ was found by FISH. Using qPCR analysis, the percentage of positive samples was lower (52.4%), and a positive association was found in samples from older patients (> 60 years). Interestingly, 71 qPCR-negative cases were detected by FISH in the presence of non-epithelial cells and in 10 qPCR-positive cases with no evidence of EBV according to FISH. The concordance between the two techniques was low, with only 57.6%. FISH is more informative for associating the gastric carcinoma with EBV positivity in tumor/epithelial cells; however, qPCR can provide relevant information regarding the progression and characteristics of neoplasia.
Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
Authors: Donnelly Christopher R. , Kumari Archana , Li Libo , Vesela Iva , Bradley Robert M. , Mistretta Charlotte M. , Pierchala Brian A. 
Published: December 2021

AbstractThe fungiform papilla (FP) is a gustatory and somatosensory structure incorporating chorda tympani (CT) nerve fibers that innervate taste buds (TB) and also contain somatosensory endings for touch and temperature. Hedgehog (HH) pathway inhibition eliminates TB, but CT innervation remains in the FP. Importantly, after HH inhibition, CT neurophysiological responses to taste stimuli are eliminated, but tactile responses remain. To examine CT fibers that respond to tactile stimuli in the absence of TB, we used Phox2b-Cre; Rosa26LSL−TdTomato reporter mice to selectively label CT fibers with TdTomato. Normally CT fibers project in a compact bundle directly into TB, but after HH pathway inhibition, CT fibers reorganize and expand just under the FP epithelium where TB were. This widened expanse of CT fibers coexpresses Synapsin-1, β-tubulin, S100, and neurofilaments. Further, GAP43 expression in these fibers suggests they are actively remodeling. Interestingly, CT fibers have complex terminals within the apical FP epithelium and in perigemmal locations in the FP apex. These extragemmal fibers remain after HH pathway inhibition. To identify tactile end organs in FP, we used a K20 antibody to label Merkel cells. In control mice, K20 was expressed in TB cells and at the base of epithelial ridges outside of FP. After HH pathway inhibition, K20 + cells remained in epithelial ridges but were eliminated in the apical FP without TB. These data suggest that the complex, extragemmal nerve endings within and disbursed under the apical FP are the mechanosensitive nerve endings of the CT that remain after HH pathway inhibition.
A mixed u–p edge-based smoothed particle finite element formulation for viscous flow simulations
Authors: Reinold Janis , Meschke Günther 
Published: December 2021

AbstractA mixed u–p edge-based smoothed particle finite element formulation is proposed for computational simulations of viscous flow. In order to improve the accuracy of the standard particle finite element method, edge-based and face-based smoothing operations on the displacement gradient are proposed for 2D and 3D analyses, respectively. Consequently, spatial integration involving the smoothing operator is performed on smoothing domains. The constitutive model is based on an elasto-viscoplastic formulation allowing for simulations of viscous fluid or fluid-like solid materials. The viscous response is modeled using an overstress function. The performance of the proposed edge-based smoothed particle finite element method (ES-PFEM) is demonstrated by several numerical benchmark studies, showing an excellent agreement with analytical and reference solutions and an improved accuracy and computational efficiency in comparison with results from the standard PFEM model. Finally, a numerical application of the ES-PFEM to the computational simulation of the extrusion process during 3D-concrete-printing is discussed.
Hydro-mechanical Response to Gas Transfer of Deep Argillaceous Host Rocks for Radioactive Waste Disposal
Authors: Gonzalez-Blanco Laura , Romero Enrique , Marschall Paul , Levasseur Séverine 
Published: December 2021

AbstractDuring recent decades, argillaceous sedimentary formations have been studied as potential host formations for the geological disposal of long-living and heat-emitting radioactive waste—Boom Clay in Belgium and Opalinus Clay and Brown Dogger in Switzerland. A significant issue in the long-term performance of these potential host rocks concerns the generation and transport of gases. The pressure resulting from the generation of gas in an almost impermeable geological medium in the near field of a repository will increase. Under high gas pressures, the mechanical and hydraulic properties of the host rock are expected to change significantly. Preferential gas pathways may develop which exploit material heterogeneity, anisotropy (bedding planes), rock discontinuities, or interfaces between the different components of the repository, and may eventually lead to the release of the produced gases. Gas flow through these clayey rocks is investigated on the basis of laboratory work. Priority has been given to studying the volume change response of these initially water-saturated materials through relatively fast and controlled volume-rate gas injections. The effect of the gas injection rate, the confining pressure and the bedding orientation on the gas transport properties have been studied with particular attention paid to the coupling with strain behaviour. The results have shown features common to the three formations concerning the gas transfer process through preferential pathways, despite their initially differential properties.
The consecutive disparity of precipitation in conterminous Spain
Authors: Martin-Vide Javier , Lopez-Bustins Joan-Albert , Lemus Marc , Moreno-Garcia M. Carmen , Balagué Xavier , Gonzalez-Hidalgo José Carlos , Beguería Santiago , Peña-Angulo Dhais , Trullenque Víctor 
Published: December 2021

AbstractPrecipitation irregularity constitutes a constraint for natural systems and socio-economic activities, particularly in water-scarce environments. Standard variability statistics such as the standard deviation, variance, and coefficient of variation do not consider the chronological order of these values. In Climatology, however, the temporal order of meteorological events is a relevant factor that can affect natural and socio-economic systems. In order to evaluate the disparity between consecutive values in precipitation series, we applied the Consecutive Disparity Index (D) to the monthly grid with the highest spatial resolution (10×10 km) existing in Peninsular Spain for the period December 1915–November 2015. Monthly, seasonal, and annual D values show an increase from north to southwest, especially in July and August. The D values for the month-to-month correlative series and for monthly mean precipitation reveal a relatively similar pattern. In the latter case, however, the low values are recorded towards southern Spain, following some mountain ranges in the Centre-East of the territory. Monthly, seasonal, and annual precipitation values are also negatively correlated with the corresponding D values.
Identification of heterogenous nuclear ribonucleoproteins (hnRNPs) and serine- and arginine-rich (SR) proteins that induce human papillomavirus type 16 late gene expression and alter L1 mRNA splicing
Authors: Hao Chengyu , Gong Lijing , Cui Xiaoxu , Jönsson Johanna , Zheng Yunji , Wu Chengjun , Kajitani Naoko , Schwartz Stefan 
Published: December 2021

AbstractWe have determined the effect of seven serine- and arginine-rich (SR) proteins and 15 heterogenous nuclear ribonucleoproteins (hnRNPs) on human papillomavirus type 16 (HPV16) late gene expression. Of the seven SR proteins analyzed here, SRSF1, SRSF3, and SRSF9 induced HPV16 late gene expression, and five of the SR proteins affected HPV16 L1 mRNA splicing. Of the 15 hnRNP proteins analyzed here, hnRNP A2, hnRNP F, and hnRNP H efficiently induced HPV16 late gene expression, and all of the hnRNPs affected HPV16 L1 mRNA levels or mRNA splicing. Thus, the majority of SR proteins and hnRNPs have the potential to regulate HPV16 L1 mRNA splicing. Strict control of the expression of the immunogenic L1 and L2 capsid proteins may contribute to the ability of HPV16 to cause persistence.
Variability of sleep bruxism—findings from consecutive nights of monitoring
Authors: Ohlmann Brigitte , Bömicke Wolfgang , Behnisch Rouven , Rammelsberg Peter , Schmitter Marc 
Published: December 2021

Abstract

Objectives
To determine sleep bruxism (SB) behavior during five consecutive nights and to identify correlations between SB episodes per hour (SB index) and sleep-time masseter-muscle activity (sMMA).

Material and methods
Thirty-one participants were included in the study. Of these, 10 were classified as sleep bruxers (group SB-1) and nine as non-sleep bruxers (group non-SB). The bruxism status of these 19 patients was identified by means of questionnaires, an assessment of clinical symptoms, and electromyographic/electrocardiographic data (Bruxoff® device). The remaining 12 participants were also identified as bruxers, but based exclusively on data from the Bruxoff device (group SB-2). Data analysis included descriptive statistics and Spearman’s correlation to assess the relationship between the SB index and sMMA.

Results
Participants in group SB-1 showed an overall mean SB index of 3.1 ± 1.6 and a mean total sMMA per night of 62.9 ± 38.3. Participants in group SB-2 had an overall mean SB index of 2.7 ± 1.5 and a mean total sMMA of 56.0 ± 29.3. In the non-SB group, participants showed an overall mean SB index of 0.8 ± 0.5 and a mean total sMMA of 56.8 ± 30.3. Spearman’s correlation yielded values of − 0.27 to 0.71 for the correlation between sMMA and SB index.

Conclusions
The data revealed variable SB activity and the absence of a reliable correlation between sMMA and the SB index.

Clinical relevance
The high variation in SB activity and lack of correlation between sMMA and the SB index should be considered when diagnosing SB.

Trial registration
Clinical Trials [NIH], clinical trial no. NCT03039985.
Research priority setting in obesity: a systematic review
Authors: Iqbal Halima , McEachan Rosemary R. C. , West Jane , Haith-Cooper Melanie 
Published: December 2021

Abstract
Aim
Obesity research priority setting, if conducted to a high standard, can help promote policy-relevant and efficient research. Therefore, there is a need to identify existing research priority setting studies conducted in the topic area of obesity and to determine the extent to which they followed good practice principles for research priority setting.

Method
Studies examining research priority setting in obesity were identified through searching the MEDLINE, PBSC, CINAHL, PsycINFO databases and the grey literature. The nine common themes of good practice in research priority setting were used as a methodological framework to evaluate the processes of the included studies. These were context, use of a comprehensive approach, inclusiveness, information gathering, planning for implementation, criteria, methods for deciding on priorities, evaluation and transparency.

Results
Thirteen articles reporting research prioritisation exercises conducted in different areas of obesity research were included. All studies reported engaging with various stakeholders such as policy makers, researchers and healthcare professionals. Public involvement was included in six studies. Methods of research prioritisation commonly included both Delphi and nominal group techniques and surveys. None of the 13 studies fulfilled all nine of the good practice criteria for research priority setting, with the most common limitations including not using a comprehensive approach and lack of inclusivity and evaluating on their processes.

Conclusion
There is a need for research priority setting studies in obesity to involve the public and to evaluate their exercises to ensure they are of high quality.
Conversos, Moriscos, and the Eucharist in Early Modern Spain: Some Reflections on Jewish Exceptionalism
Authors: Glazer-Eytan Yonatan 
Published: December 2021

AbstractSacrilegious attitudes toward the Eucharistic host are one of the most commonplace accusations leveled against Jews in premodern Europe. Usually treated in Jewish historiography as an expression of anti-Judaism or antisemitism, they are considered a hallmark of Jewish powerlessness and persecution. In medieval and early modern Spain, however, Jews and conversos (Jewish converts to Christianity and their descendants) were not the only proclaimed enemies of the Eucharist. Reports about avoidance, rejection, criticism, and even ridicule and profanation of the consecrated host were similarly leveled against Muslims and moriscos (Muslim converts to Christianity). This essay seeks to assess the parallels and connections between the two groups through a comparative examination of accusations of sacrilegious behavior towards the host. The first part of the essay analyzes religious art, legal compendia, and inquisitorial trials records from the tribunals of Toledo and Cuenca in order to show some evident homologies between the two groups. The second part of the essay focuses on the analysis of the works of Jaime Bleda and Pedro Aznar y Cardona, two apologists of the expulsion of the moriscos, and draws direct connections between Jewish and morisco sacrilege. By exploring the similarities and differences between accusations against conversos and moriscos, this essay aims to offer a broader reflection on Jewish exceptionalism.
Investigation of per- and polyfluoroalkyl substances (PFAS) in soils and sewage sludges by fluorine K-edge XANES spectroscopy and combustion ion chromatography
Authors: Roesch Philipp , Vogel Christian , Huthwelker Thomas , Wittwer Philipp , Simon Franz-Georg 
Published: December 2021

AbstractFor the first time, fluorine K-edge X-ray absorption near-edge structure (XANES) spectroscopy was applied to detect per- and polyfluoroalkyl substances (PFAS) in various soil and sewage sludge samples. The method can be used to determine the speciation of inorganic and organic fluorides, without pre-treatment of solid samples. Therefore, XANES spectra of several inorganic fluorides as well as selected fluorinated organic compounds were recorded. While inorganic fluorides partially exhibit a variety of sharp spectral features in the XANES spectrum, almost all inspected organofluorine compounds show two distinct broad features at 688.5 and 692.0 eV. Moreover, the peak intensity ratio 688.5 eV/692.0 eV in the PFAS XANES spectrum can be inversely correlated to the chain length of the perfluoro sulfonic acid group. The detection of targeted PFAS by bulk-XANES spectroscopy in combination with linear combination fitting in soils and sewage sludges was not applicable due to the low organic fluorine to total fluorine ratio of the samples (0.01–1.84%). Nonetheless, direct analysis of pure PFAS revealed that analysis of organofluorine species might be achieved in higher concentrated samples. Furthermore, quantitative measurements by combustion ion chromatography (CIC) evaluated as sum parameters extractable organically bound fluorine (EOF) and total fluorine (TF) emphasize that besides soils, sewage sludges are a significant source of organic fluorine in agriculture (154–7209 µg/kg).
Correlation of plasma and urine Wnt5A with the disease activity and cutaneous lesion severity in patients with systemic lupus erythematosus
Authors: Chi Shuhong , Xue Jing , Chen Xiaodong , Liu Xiaoming , Ji Yanhong 
Published: December 2021

AbstractReliable noninvasive biomarkers are needed to accurately assess disease activity and prognosis in patients with systemic lupus erythematosus (SLE). The purpose of this study was to investigate the clinical relevance of Wnt5A with disease activity and severity with cutaneous involvement in particular in SLE patients; its concentrations in plasma and urine were examined and analyzed. In the cross-sectional study, the clinical relevance of Wnt5A protein was evaluated in both plasma and urine of SLE patients and healthy cohorts using commercial enzyme-linked immunosorbent assays (ELISA). Significantly, more abundances of Wnt5A protein were determined in both of plasmas and urines of SLE patients compared to healthy cohorts (p < 0.0001), which were even higher in active disease (AD) SLE patients relative to low disease activity (LDA) SLE patients (p < 0.0001). Meanwhile, the ROC curve analysis demonstrated that the plasma and urine Wnt5A were potential candidate biomarkers for identifying the disease activity and severity in SLE patients. The discriminant function analysis further revealed that the plasma and urine Wnt5A were separated and distinct for AD SLE patients and healthy controls. In consistence, the disease severity was correlated with the plasma and urine Wnt5A as ascertained by CLASI activity score and the prevalence of serositis in SLE patients. These results suggest that Wnt5A, as a summary measure for different inflammatory processes, could be a potential biomarker for accessing the disease activity, and a noninvasive biomarker for evaluating the disease severity in terms of cutaneous involvement in SLE patients.
Archetypes of Digital Twins
Authors: van der Valk Hendrik , Haße Hendrik , Möller Frederik , Otto Boris 
Published: December 2021

AbstractCurrently, Digital Twins receive considerable attention from practitioners and in research. A Digital Twin describes a concept that connects physical and virtual objects through a data linkage. However, Digital Twins are highly dependent on their individual use case, which leads to a plethora of Digital Twin configurations. Based on a thorough literature analysis and two interview series with experts from various electrical and mechanical engineering companies, this paper proposes a set of archetypes of Digital Twins for individual use cases. It delimits the Digital Twins from related concepts, e.g., Digital Threads. The paper delivers profound insights into the domain of Digital Twins and, thus, helps the reader to identify the different archetypical patterns.
Influence of socioeconomic variables on physical activity and screen time of children and adolescents during the COVID‑19 lockdown in Germany: the MoMo study
Authors: Schmidt Steffen C. E. , Burchartz Alexander , Kolb Simon , Niessner Claudia , Oriwol Doris , Woll Alexander 
Published: December 2021

AbstractThe COVID‑19 (coronavirus disease 2019) pandemic created a multitude of natural experiments about the change of human behavior in a widely unfamiliar situation. Besides physical and mental health, physical activity (PA) and people’s movement behaviors were of particular interest to researchers all over the world. In a recent study, we found that among youth in Germany, sports activity declined, whereas recreational screen time and habitual activity increased during the first COVID‑19 lockdown. In the present study, we analyze the influence of the socioeconomic status and the housing situation on the changes in PA behavior and recreational screen-time before and during the first COVID‑19 lockdown among children and adolescents living in Germany. We found an alignment of PA behavior among youth from families with different socioeconomic backgrounds during the first lockdown and identified the housing situation to be a meaningful predictor of the increase in habitual activity. We conclude that restriction policies, communities, and in the last instance parents need to enable access to nonorganized PA to all children and adolescents every day and especially during potential future lockdowns.