bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired photoprotection in Phaeodactylum tricornutum KEA3 mutants reveals the proton regulatory circuit of diatoms light acclimation
Authors: Seydoux Claire , Storti Mattia , Giovagnetti Vasco , Matuszyńska Anna , Guglielmino Erika , Zhao Xue , Giustini Cécile , Pan Yufang , Angulo Jhoanell , Ruban Alexander V. , Hu Hanhua , Bailleul Benjamin , Courtois Florence , Allorent Guillaume , Finazzi Giovanni 
Published: September 2021

AbstractDiatoms are amongst the most successful clades of oceanic phytoplankton, significantly contributing to photosynthesis on Earth. Their ecological success likely stems from their ability to acclimate to changing environmental conditions, including e.g. variable light intensity. Diatoms are outstanding at dissipating light energy exceeding the maximum photosynthetic electron transfer (PET) capacity of via Non Photochemical Quenching (NPQ). While the molecular effectors of this process, as well as the role of the Proton Motive Force (PMF) in its regulation are known, the putative regulators of the PET/PMF relationship in diatoms remain unidentified. Here, we demonstrate that the H+/K+ antiporter KEA3 is the main regulator of the coupling between PMF and PET in the model diatom Phaeodactylum tricornutum. By controlling the PMF, it modulates NPQ responses at the onset of illumination, during transients and in steady state conditions. Under intermittent light KEA3 absence results in reduced fitness. Using a parsimonious model including only two components, KEA3 and the diadinoxanthin de-epoxidase, we can describe most of the feedback loops observed between PET and NPQ. This two-components regulatory system allows for efficient responses to fast (minutes) or slow (e.g. diel) changes in light environment, thanks to the presence of a regulatory Ca2+-binding domain in KEA3 that controls its activity. This circuit is likely finely tuned by the NPQ effector proteins LHCX, providing diatoms with the required flexibility to thrive in different ocean provinces.One sentence summaryThe author(s) responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (https://academic.oup.com/plcell/pages/General-Instructions) is Giovanni Finazzi.
Leishmania Hijacks microRNA Import-Export Machinery of Infected Macrophage and Survives by Cross-Communicating Host miR-146a to Subjugate HuR and miR-122 in Neighbouring cells
Authors: Ganguly Satarupa , Ghoshal Bartika , Banerji Ishani , Bhattacharjee Shreya , Chakraborty Sreemoyee , Goswami Avijit , Mukherjee Kamalika , Bhattacharyya Suvendra N. 
Published: September 2021

AbstractLeishmania donovani, the causative agent of visceral leishmaniasis, infects and resides within tissue macrophage cells of the mammalian host. It is not clear how the parasite infected cells cross-talk with the non-infected cells in the infection niche to regulate the infection process. Interestingly, miRNAs, the regulatory small RNAs of the host, could get trafficked into and out of infected cells as part of extracellular vesicles to ensure exchange of the epigenetic signals and can regulate the expression of their target genes in both donor and recipient cells.Leishmania, for its survival in host macrophage, adopts a dual strategy to regulate the intercellular transport of host miRNAs. The parasite, by preventing mitochondrial function of the host cells, restricts the entry of liver cell derived miR-122 containing extracellular vesicles in infected macrophage to curtail the inflammatory response by miR-122. The parasite reciprocally upregulates the extracellular export of anti-inflammatory miR-146a from the infected cells. The exported miR-146a restricts miR-122 production in liver cells and polarizes neighbouring naïve macrophages to the M2 state. miR-146a upregulates IL-10 in neighbouring macrophages where miR-146a dominates the RNA binding and miRNA suppressor protein HuR to inhibit the expression of proinflammatory cytokine mRNAs having HuR-interacting AU-rich elements and polarized the recipient cells to M2 stage.Graphical AbstractKey FindingsLeishmaniastops vesicular entry of inflammatory miR-122 in infected cells by causing mitochondrial depolarizationLeishmaniasecrets miR-146a from infected cells to stop miR-122 production in neighbouring hepatocytesmiR-146a containing vesicles secreted by infected cells stops inflammatory response in recipient naïve macrophagemiR-146 targets RNA binding protein HuR to stop inflammatory cytokine production
Genome-wide association mapping of ethanol sensitivity in the Diversity Outbred mouse population
Authors: Parker Clarissa C. , Philip Vivek M. , Gatti Daniel M. , Kasparek Steven , Kreuzman Andrew M. , Kuffler Lauren , Mansky Benjamin , Masneuf Sophie , Sharif Kayvon , Sluys Erica , Taterra Dominik , Taylor Walter M. , Thomas Mary , Polesskaya Oksana , Palmer Abraham A. , Holmes Andrew , Chesler Elissa J. 
Published: September 2021

AbstractBackgroundA strong predictor for the development of alcohol use disorders (AUDs) is altered sensitivity to the intoxicating effects of alcohol. Individual differences in the initial sensitivity to alcohol are controlled in part by genetic factors. Mice offer a powerful tool for elucidating the genetic basis of behavioral and physiological traits relevant to AUDs; but conventional experimental crosses have only been able to identify large chromosomal regions rather than specific genes. Genetically diverse, highly recombinant mouse populations allow for the opportunity to observe a wider range of phenotypic variation, offer greater mapping precision, and thus increase the potential for efficient gene identification.MethodsWe have taken advantage of the Diversity Outbred (DO) mouse population to identify and precisely map quantitative trait loci (QTL) associated with ethanol sensitivity. We phenotyped 798 male J:DO mice for three measures of ethanol sensitivity: ataxia, hypothermia, and loss of the righting response. We used high density MEGAMuga and GIGAMuga arrays to obtain genotypes ranging from 77,808 – 143,259 SNPs. In addition, we performed RNA sequencing in striatum to map expression QTLs and to identify gene expression-trait correlations.ResultsWe then applied a systems genetic strategy to identify narrow QTLs and construct the network of correlations that exist between DNA sequence, gene expression values and ethanol-related phenotypes to prioritize our list of positional candidate genes.ConclusionsOur results can be used to identify alleles that contribute to AUDs in humans, elucidate causative biological mechanisms, or assist in the development of novel therapeutic interventions.
Blocking a dead-end assembly pathway creates a point of regulation for the DEAD-box ATPase Has1 and prevents platform misassembly
Authors: Liu Xin , Huang Haina , Karbstein Katrin 
Published: September 2021

AbstractAssembly of ribosomal subunits occurs via parallel pathways, which accelerate the process and render it more robust. Nonetheless, in vitro analyses have also demonstrated that some assembly pathways are dead-ends, presumably due to rRNA misfolding. If and how these non-productive pathways are avoided during assembly in vivo remains unknown. Here we use a combination of biochemical, genetic, proteomic and structural analyses to demonstrate a role for assembly factors in biasing the folding landscape away from non-productive intermediates. By binding Rrp36, Rrp5 is prevented from forming a premature interaction with the platform, which leads to a dead-end intermediate, and a misassembled platform that is functionally defective. The DEAD-box ATPase Has1 separates Rrp5 and Rrp36, allowing Rrp5 to reposition to the platform, thereby promoting ribosome assembly and enabling rRNA processing. Thus, Rrp36 establishes an ATP-dependent regulatory point that ensures correct platform assembly by opening a new folding channel that avoids funnels to misfolding.
The effect of taxes, similar financial liabilities and demographic variables on Turkey's table wine market: a survey
Authors: Tosun Ayse Nil , Yilmaz Ayfer Ezgi 
Published: September 2021

PurposeThis study examines the effect of taxes, similar financial liabilities, and demographic variables such as respondent age, company age, and field of operation on the table wine market in Turkey.Design/methodology/approachAn online survey was conducted on the wine producers and importers via SurveyMonkey. Thirty-six survey questions were answered using a five-point Likert scale. The responses obtained from 51 owners and administrators of wine companies were analyzed using the Statistical Package for the Social Sciences 23 program.FindingsExcise and value-added taxes affected the amount and price of table wine production in Turkey, whereas the banderole affected quality and price. The excise tax, value-added tax, banderole, Resource Utilization Support Fund, customs duty, and authorizations also affected the amount, quality and price of table wine imports. Although financial liabilities such as the banderole, Resource Utilization Support Fund and authorizations required for import do not constitute a heavy load on wine costs, they do have similar effects as other taxes on table wine imports.Research limitations/implicationsA limitation of this study was its sample size. Only 51 individuals responded, as it was an online questionnaire. However, this did not compromise the representativeness of the sample with regard to the company's field of operation (production and import of table wine), lending credibility to the opinions provided.Originality/valueThis study establishes that, contrary to popular belief, decisions regarding wine production and import are not solely affected by a heavy tax burden. Other factors, such as the banderole, Resource Utilization Support Fund, ages of companies and respondents and authorizations required for import, which are not viewed as a heavy burden in monetary terms, also prove to be decisive.
What drives clients to purchase green building?: The cybernetic fuzzy analytic hierarchy process approach
Authors: Durdyev Serdar , Mohandes Saeed Reza , Mahdiyar Amir , Ismail Syuhaida 
Published: September 2021

PurposeHomebuyers' motivation to purchase the green building (GB) is vital for the widespread adoption of greener practices and for tackling the adverse impact of the built environment. In this regard, through the lenses of qualified and competent GB developers (GBD) and real estate agents (REAs) using judgment sampling technique, this paper aims to evaluate the factors stimulating the purchase intention of Malaysian homebuyers.Design/methodology/approachThe study uses the interval-valued fuzzy Delphi method (IVFDM) to identify influential factors, while the cybernetic fuzzy analytic hierarchy process (CFAHP) for prioritizing the identified factors according to their significance in nationwide promotion of GBs is utilized.FindingsThe findings, which are consistent with those reported in the literature and validated by the focus group, uncover the most prominent factors that drive the purchase intention of GB homebuyers. The results indicate that the homebuyers are familiar with the benefits of GB; however, the benefits are not sufficient to overcome the perceived “high cost” of green practices. Hence, homebuyers still seek financial incentives, which is a clear indication that financial issues are the most motivating factor.Practical implicationsIncrease in informative promotion of GB is necessary, particularly among prospective homebuyers in developing countries where GB adoption is in its infancy. Frontline players (those who are responsible for wider promotion) can utilize the factors that this study found to be important to identify appropriate steps to increase prospective homebuyers' motivation for purchasing greener dwellings.Originality/valueThe contribution of this paper is threefold. First, review and identification of the most cited determinants that have been reported in the context of the subject. Second, this paper presents the most significant determinants in a developing country setting through the lenses of Malaysian experts in the area. Third, improvement measures could be implemented in Malaysia and other developing countries to promote the GB paradigm with an aim to make the building environment more sustainable.
Does immigration affect residential real estate prices? Evidence from Australia
Authors: Gopy-Ramdhany Narvada , Seetanah Boopen 
Published: September 2021

Purpose
This study aims to investigate the effect of immigration on housing prices in Australia both at the national and regional levels.

Design/methodology/approach
Data for eight Australian states on a quarterly basis from 2004–2017 is used. To study the possible dynamic and endogenous relationship between housing prices and immigration, a panel vector autoregressive error correction model (PVECM) is adopted.

Findings
Analysis of the results indicates that in the short run immigration positively and significantly affects housing prices, whereas in the long run no significant relationship was observed between the two variables. From the regional breakdown and analysis, it is discerned that in some states there is a significant and positive effect of immigration on residential real estate prices in the long run. Causality analysis confirms that the direction of causation is from immigration to housing prices.

Practical implications
The study illustrates that immigration and interstate migration, as well as high salaries, have been causing a rise in housing demand and subsequently housing prices. To monitor exceedingly high housing prices, local authorities should be controlling migration and salary levels.

Originality/value
Past research studies had highlighted the importance of native interstate migration in explaining the nexus between immigration – housing prices. In this study, it has been empirically verified how immigration has been affecting the locational decisions of natives and subsequently how this has been affecting housing prices.
CEO pay slice and acquisitions in Australia: the role of tournament incentives
Authors: Luong Hoa , Gunasekarage Abeyratna , Shams Syed 
Published: September 2021

PurposeThis paper investigates the influence of tournament incentives, measured by Chief Executive Officer (CEO) pay slice (CPS), on the acquisition decisions of Australian firms.Design/methodology/approachThis study applies ordinary least squares regression analyses to a sample of 1,429 acquisition observations announced by 986 unique Australian firms spanning the 2001–2015 period. Event study methodology was employed to capture the market reaction to acquisition announcements. Multinomial logit models, a two-stage least squares instrumental variable (IV) approach and propensity score matching (PSM) technique were performed for robustness and endogeneity correction purposes.FindingsThe results suggest that CPS has a positive and significant relationship with the announcement period abnormal return realised by acquirers, implying that executives are motivated to exert best efforts and support the CEO in making value-creating acquisitions. Further analyses reveal that management teams of high CPS firms demonstrate efficiencies in executing acquisitions. The positive relationship between the CPS and abnormal return is more pronounced in acquisitions of private targets, domestic targets and bidders with high-quality CEOs. These acquisitions make a significant contribution to the long-run performance of the firm, which provides support for the effort inducement hypothesis.Practical implicationsThe study's empirical evidence implies that the strong governance environment in Australia and a highly monitored acquisition market and compensation contracts motivates executives to exert their efforts to make value-enhancing acquisitions.Originality/valueThis paper appears to be the first investigation that makes a link between CPS in different components (i.e. short-term, long-term and total pay) as proxy for tournament incentives and the outcomes of both public and non-public acquisitions in the Australian setting.
Perceptions of online self- and peer-assessment: accounting students in a large undergraduate cohort
Authors: Pidduck Teresa Michelle , Bauer Nadia 
Published: September 2021

PurposeSelf-assessment (SA) and peer-assessment (PA) are considered useful tools in the development of lifelong learning and reflective skills. The authors implemented a teaching intervention using SA and PA amongst a large cohort of final year undergraduate students. The purpose of this study was to investigate students' perceptions of online SA and PA in order to understand the differences between these perceptions and to allow instructors to adopt differentiated instruction in developing a diverse student group's professional skills.Design/methodology/approachThe research design adopted a mixed methods approach through the use of surveys that were administered before and after the SA and peer-assessment intervention in a taxation module taught at a large public South African university. Through the use of a series of open and closed questions students' perceptions on SA and peer-assessment were analysed both quantitatively and qualitatively.FindingsThe findings show that student perceptions of SA and peer-assessment differed significantly, where perceptions of SA were more positive than those towards PA. The findings indicate that SA and peer-assessment still present a challenge in an online context for large student cohorts, despite improved tracking, faster feedback and anonymity.Originality/valueThe study contributes to the literature by analysing students' perceptions about SA and peer-assessment in an accounting education context and in an online setting in South Africa.
(En) act your age! Marketing and the marketization of history in young SMEs
Authors: Weatherbee Terrance , Sears Donna 
Published: September 2021

PurposeThis paper aims to examine how wineries used history in their marketing communications to overcome the liability of newness in a settled field that valorizes duration and longevity.Design/methodology/approachA multiple-case study investigated the treatment of history in marketing by young wineries in a new wine region. Data included interviews, site visits and marketing communications.FindingsWineries worked to communicate stakeholder legitimacy and authenticity by constructing organizational histories through bricolage, communicating history in symbolic, material and practice forms.Research limitations/implicationsYoung organizations can communicate field legitimacy and projections of organizational and product authenticity through constructed histories. Results may not be generalizable to other jurisdictions as wine marketing is normatively subject to government regulation. The importance of history in marketing communications also varies across sectors.Practical implicationsYoung businesses in sectors where tradition, place and longevity are venerated can establish authenticity and legitimacy through the marketization of history by following practices that demonstrate adherence to tradition and making thoughtful choices in the construction of the symbolic and material aspects of their organizations.Originality/valueThis study demonstrates that new/young organizations can use bricolage to create their own marketized histories as proxies for age.
Case-wise analysis of Love-type wave propagation in an irregular fissured porous stratum coated by a sandy layer
Authors: Gupta Shishir , Das Soumik , Dutta Rachaita 
Published: September 2021

PurposeThe purpose of the present study is to investigate the dispersion and damping behaviors of Love-type waves propagating in an irregular fluid-saturated fissured porous stratum coated by a sandy layer.Design/methodology/approachTwo cases are analyzed in this study. In case-I, the irregular fissured porous stratum is covered by a dry sandy layer, whereas in case-II, the sandy layer is considered to be viscous in nature. The method of separation of variables is incorporated in this study to acquire the displacement components of the considered media.FindingsWith the help of the suitable boundary conditions, the complex frequency relation is established in each case leading to two distinct equations. The real and imaginary parts of the complex frequency relation define the dispersion and attenuation properties of Love-type waves, respectively. Using the MATHEMATICA software, several graphical implementations are executed to illustrate the influence of the sandiness parameter, total porosity, volume fraction of fissures, fluctuation parameter, flatness parameters and ratio of widths of layers on the phase velocity and attenuation coefficient. Furthermore, comparison between the two cases is clearly framed through the variation of aforementioned parameters. Some particular cases in the presence and absence of irregular interfaces are also analyzed.Originality/valueTo the best of the authors' knowledge, although many articles regarding the surface wave propagation in different crustal layers have been published, the propagation of Love-type waves in a sandwiched fissured porous stratum with irregular boundaries is still undiscovered. Results accomplished in this analytical study can be employed in different practical areas, such as earthquake engineering, material science, carbon sequestration and seismology.
Transforming social studies curriculum to integrate a social justice framework
Authors: Popp Jacquelynn S. , Montgomery Josh , Hoard Jodi , Brock Cynthia 
Published: September 2021

PurposeThe purpose of this paper is to empower teachers to engage in a process of curricular transformation to integrate a social justice framework, even if it means starting with small steps.Design/methodology/approachThe authors present a set of guiding principles on which social studies teachers can draw to transform their curriculum to embody a social justice framework within and across units of historical inquiry. The principles are anchored in an example historical unit, the Chicago Haymarket Affair of 1886, and an analogous contemporary sub-unit, The Exonerated Five (formerly The Central Park Five incident of 1989).FindingsThe guiding principles represent an accessible approach educators can flexibly apply to their process of curricular transformation. The authors provide a balanced approach of emphasizing the need for educators to restructure social studies curriculum with the feasibility of this process at larger or smaller scales according to educators' readiness for change.Originality/valueThe authors outline a process to empower teachers to change the status quo of their social studies teaching, at a scale determined by the teacher. The authors provide a practical, concrete set of guiding principles for educators to make changes that represent social justice integration aligned with existing social studies curriculum and standards. The authors encourage teachers to reflect on their readiness for and progress toward transforming their curriculum to integrate a social justice framework.