bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin inhibits ovarian cancer progression by regulating circ-PLEKHM3/miR-320a/SMG1 axis
Authors: Sun Sifan , Fang Hailiang 
Published: November 2021

Abstract
Background
Curcumin has a potential therapeutic role in ovarian cancer. However, whether curcumin plays anti-cancer role in ovarian cancer by mediating the circular RNA (circRNA)/microRNA (miRNA)/mRNA network is still unclear.

Methods
The expression of circ-PLEKHM3, miR-320a, and suppressor of morphogenesis in genitalia 1 (SMG1) was detected via qRT-PCR. Cell viability, colony-formation ability and apoptosis were analyzed via cell counting kit-8 assay, colony formation analysis, and flow cytometry. Protein expression was measured using western blot. The in vivo experiments were performed using a xenograft model. Target association was evaluated via dual-luciferase reporter analysis and RIP assay.

Results
Curcumin suppressed ovarian cancer cell proliferation and promoted apoptosis. Circ-PLEKHM3 was downregulated in ovarian cancer, and its expression could be promoted by curcumin treatment. Circ-PLEKHM3 overexpression exacerbated the effect of curcumin on ovarian cancer cell proliferation and apoptosis, as well as anti-tumor effect. MiR-320a was targeted by circ-PLEKHM3. The inhibition effect of circ-PLEKHM3 overexpression on cell proliferation and the enhancing effect on cell apoptosis could be reversed by miR-320a mimic. SMG1 was targeted by miR-320a, and its knockdown also reversed the regulation of miR-320a inhibitor on the proliferation and apoptosis of ovarian cancer cells. In addition, circ-PLEKHM3 could upregulate SMG1 expression via sponging miR-320a.

Conclusion
Curcumin restrained proliferation and facilitated apoptosis in ovarian cancer by regulating the circ-PLEKHM3/miR-320a/SMG1 axis.
The STUN (STop UNhealthy) Alcohol Use Now trial: study protocol for an adaptive randomized trial on dissemination and implementation of screening and management of unhealthy alcohol use in primary care
Authors: Jonas Daniel E. , Barclay Colleen , Grammer Debbie , Weathington Chris , Birken Sarah A. , DeWalt Darren A. , Shoenbill Kimberly A. , Boynton Marcella H. , Mackey Monique , Riley Sean , Cykert Samuel 
Published: November 2021

Abstract
Background
Unhealthy alcohol use is a leading cause of preventable deaths in the USA and is associated with many societal and health problems. Less than a third of people who visit primary care providers in the USA are asked about or ever discuss alcohol use with a health professional.

Methods/design
This study is an adaptive, randomized, controlled trial to evaluate the effect of primary care practice facilitation and telehealth services on evidence-based screening, counseling, and pharmacotherapy for unhealthy alcohol use in small-to-medium-sized primary care practices. Study participants will include primary care practices in North Carolina with 10 or fewer providers. All enrolled practices will receive a practice facilitation intervention that includes quality improvement (QI) coaching, electronic health record (EHR) support, training, and expert consultation. After 6 months, practices in the lower 50th percentile (based on performance) will be randomized to continued practice facilitation or provision of telehealth services plus ongoing facilitation for the next 6 months. Practices in the upper 50th percentile after the initial 6 months of intervention will continue to receive practice facilitation alone. The main outcome measures include the number (and %) of patients in the target population who are screened for unhealthy alcohol use, screen positive, and receive brief counseling. Additional measures include the number (and %) of patients who receive pharmacotherapy for AUD or are referred for AUD services. Sample size calculations determined that 35 practices are needed to detect a 10% increase in the main outcome (percent screened for unhealthy alcohol use) over 6 months.

Discussion
A successful intervention would significantly reduce morbidity among adults from unhealthy alcohol use by increasing counseling and other treatment opportunities. The study will produce important evidence about the effect of practice facilitation on uptake of evidence-based screening, counseling, and pharmacotherapy for unhealthy alcohol use when delivered on a large scale to small and medium-sized practices. It will also generate scientific knowledge about whether embedded telehealth services can improve the use of evidence-based screening and interventions for practices with slower uptake. The results of this rigorously conducted evaluation are expected to have a positive impact by accelerating the dissemination and implementation of evidence related to unhealthy alcohol use into primary care practices.

Trial registration
ClinicalTrials.govNCT04317989. Registered on March 23, 2020.
Comparing the effects of dexmedetomidine versus propofol on the treatment of emergence agitation in adult patients after general anesthesia: study protocol for a randomized, superiority, controlled trial (DP-TEA Trial)
Authors: Feng Zhaoyan , Shi Xiao , Yan Xue , Zhu Yamin , Gu Juan , Zhu Hao , Yu Weifeng , Zhang Song 
Published: November 2021

Abstract
Background
Emergence agitation (EA) after general anesthesia is a common complication in the post-anesthesia care unit (PACU). Once EA occurs, there are still no guidelines established for the treatment in adults. Propofol is excessively used in managing agitated patients in the PACU, but it lacks analgesia and can result in apnea. Intraoperative infusion of dexmedetomidine has been proven to have a preventive effect on EA, but the treatment effect of dexmedetomidine on EA remains unknown. This study aims to compare the effects between dexmedetomidine and propofol on relieving EA in adult patients after general anesthesia in the PACU.

Methods
In this randomized, superiority, controlled clinical study, a total of 120 adult patients aged 18–65 years of both genders, with American Society of Anesthesiologists (ASA) classification I or II developing EA in the PACU after general anesthesia, will be enrolled. Patients will be randomized at a 1:1 ratio into two groups, receiving either a single dose of dexmedetomidine (0.7μg/kg) or propofol (0.5 mg/kg). The primary outcome is the proportion of patients having a recurrent EA within 15 min after intervention in the PACU.

Discussion
Previous studies have focused on premedication for preventing EA, while therapeutics for reliving EA have rarely been reported. To our knowledge, this study is the first randomized, superiority, controlled trial to compare a bolus of dexmedetomidine with the current routine care for this indication.

Trial registration
ClinicalTrials.govNCT04142840. Registered on October 26, 2019
Successful induction treatment of bullous pemphigoid using reslizumab: a case report
Authors: Rhyou Hyo-In , Han Song-Hee , Nam Young-Hee 
Published: November 2021

Abstract
Background
Bullous pemphigoid (BP) is a potentially life-threatening autoimmune blistering disease which is characterized by autoantibodies against hemidesmosomal proteins of the skin and mucous membranes. In recent years, the role of eosinophil and immunoglobulin E autoantibodies have been further elucidated in BP, and have been considered potential therapeutic targets.

Case presentations
A 67-year-old male presented with erythematous bullous eruption. The skin eruption was located on whole body, and suggested BP. Peripheral blood eosinophil count and total immunoglobulin E markedly elevated in initial laboratory findings. Topical and systemic steroid (methylprednisolone 2 mg/kg/day) treatment was started, and his skin symptoms worsened repeatedly, whenever systemic steroid were reduced. On admission day 29, reslizumab (anti-interleukin-5) 3.5 mg/kg was administered intravenously to the patients. The bullous skin lesion began to improve rapidly, and methylprednisolone (8 mg/day) was reduced without any worsening of symptoms during two doses of reslizumab.

Conclusions
We report a case of successful treatment response to reslizumab administration in a patient with BP. Further studies are needed to confirm the role of anti-interleukin-5 as a treatment for BP in the future.
Classification of nonnegative solutions to static Schrödinger–Hartree–Maxwell system involving the fractional Laplacian
Authors: Li Yunting , Liu Yaqiong , Yi Yunhui 
Published: November 2021

AbstractThis paper is mainly concerned with the following semi-linear system involving the fractional Laplacian: $$ \textstyle\begin{cases} (-\Delta )^{\frac{\alpha }{2}}u(x)= (\frac{1}{ \vert \cdot \vert ^{\sigma }} \ast v^{p_{1}} )v^{p_{2}}(x), \quad x\in \mathbb{R}^{n}, \\ (-\Delta )^{\frac{\alpha }{2}}v(x)= (\frac{1}{ \vert \cdot \vert ^{\sigma }} \ast u^{q_{1}} )u^{q_{2}}(x), \quad x\in \mathbb{R}^{n}, \\ u(x)\geq 0,\quad\quad v(x)\geq 0, \quad x\in \mathbb{R}^{n}, \end{cases} $$

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Impacts of weaning age on dietary needs of whey permeate for pigs at 7 to 11 kg body weight
Authors: Jang Ki Beom , Duarte Marcos Elias , Purvis Jerry M. , Kim Sung Woo 
Published: November 2021

Abstract
Background
Whey permeate is an effective lactose source for nursery pigs and the most benefits are obtained when pigs are at 7 to 11 kg BW. Altering weaning ages could cause different length of early-weaner phases until 7 kg BW and thus it would influence the dietary need of whey permeate during 7 to 11 kg BW of pigs. This study aimed to evaluate if weaning ages would affect the dietary needs of whey permeate for optimum growth performance of pigs at 7 to 11 kg BW.

Methods
A total of 1,632 pigs were weaned at d 21 (d 21.2 ± 1.3) or d 25 (d 24.6 ± 1.1) after birth. All pigs had a common early-weaner feeds until they reached 7 kg BW. When pigs reached 7 kg BW within a weaning age group, they were allotted in a randomized complete block design (2 × 4 factorial). Two factors were weaning age groups (21 and 25 d of age) and varying whey permeate levels (7.50%, 11.25%, 15.00%, and 18.75%). Data were analyzed using the GLM and NLIN procedures of SAS for slope-ratio and broken-line analyses to determine the growth response to whey permeate and optimal daily whey permeate intake for the growth of the pigs weaned at different ages.

Results
Pigs weaned at 21 d of age had a common diet for 11 d to reach 7 kg BW whereas pigs weaned at 25 d of age needed 2 d. The G:F of pigs weaned at 25 d of age responded to increased daily whey permeate intake greater (P < 0.05) than pigs weaned at 21 d of age. Breakpoints were obtained (P < 0.05) at 88 and 60 g/d daily whey permeate intake or 17.0% and 14.4% of whey permeate for G:F of pigs weaned at 21 and 25 d of age, respectively.

Conclusion
Pigs weaned at an older age with a short early-weaner phase had a greater growth response to whey permeate intake compared with pigs weaned at a younger age with a long early-weaner phase. Altering weaning ages affected dietary needs of whey permeate for optimum growth performance of pigs from 7 to 11 kg BW.
Online residency training during the COVID-19 pandemic: A national survey of otolaryngology head and neck surgery program directors
Authors: Chénard-Roy Jade , Guitton Matthieu J. , Thuot François 
Published: November 2021

Background The COVID-19 pandemic has deeply impacted healthcare and education systems, including resident education. The impact of the pandemic on the different types of pedagogical activities, and the displacement of pedagogical activities to online modalities have not yet been quantified. We sought to evaluate the impact of the COVID-19 pandemic on formal pedagogic components of otorhinolaryngology–head and neck surgery (ORL–HNS) residency, the switch to distance learning and program director's perceptions of the future of teaching and learning. Methods A nationwide online survey was conducted on Canadian ORL–HNS program directors. The use of standard didactic activities in-person and online, before and during the pandemic was rated with Likert scales. Perceptions of the pandemic were described with open-ended questions. Results A total of 11 of the 13 program directors contacted responded. The analysis were conducted using nonparametric statistics. There was a significant drop in overall didactic activities during the pandemic, regardless of the teaching format (3.5 ± 0.2 to 3.1 ± 0.3, p < 0.05). The most affected activities were simulation and in-house lectures. Online activities increased dramatically (0.5 ± 0.2 to 5.0 ± 0.5, p < 0.001), including attendance to lectures made by other programs (0.5 ± 0.3 to 4.0 ± 0.8, p < 0.05). Respondents stated their intention to maintain the hybrid online and in-person teaching model. Conclusions These findings suggest that hybrid online and in-person teaching is likely to persist in the post-pandemic setting. A balanced residency curriculum requires diversity in academic activities. The pandemic can have positive consequences if higher education institutions work to better support distance teaching and learning. Graphical Abstract
The effects of soil temperature from soil mulching and harvest age on phenol, flavonoid and antioxidant contents of Java tea (Orthosiphon aristatus B.)
Authors: Komariah  , Pitaloka Diora Diah Ayu , Batubara Irmanida , Nurcholis Waras , Sandrawati Apong , Setyawati Andriyana , Syamsiyah Jauhari , Dewi Widyatmani Sih 
Published: November 2021

AbstractBackgroundThe environmental conditions resulted by the agronomic management practices may govern the secondary metabolite contents of medicinal plants, including Java tea (Orthosiphon aristatusB). Abiotic factors such as temperatures have been known to determine the secondary metabolite contents of Java tea. This study aimed at evaluating the effects of soil temperature resulting from soil mulching and harvest age on total phenol, flavonoid and antioxidant contents of Java tea.MethodsThe research was arranged using nested (hierarchy design) with completely randomized design under a screen house at Karanganyar, Indonesia, from July to December 2019. The main factor was soil mulching (control; black plastic mulch, transparent plastic mulch, biodegradable mulch and rice straw mulch) with three replicates. The main factor was nested in the temporal hierarchy factor, namely harvest age which consisted of two levels, i.e., 80 and 100 days. The observation parameters were soil temperature of 10 min (maximum; mean; minimum and soil temperature-based Growing Degree Days, GDD) with sensors and logger; plant growth (plant height, number of leaves, fresh and dry weight); and secondary metabolites including phenol, flavonoids and antioxidant.ResultsThe results confirmed the order of the highest to the lowest mean soil temperature was resulted under the transparent plastic mulch > straw > black plastic mulch > control > biodegradable plastic mulch (26.69 > 26.29 > 26.10 > 26.07 > 25.68 °C, respectively). Overall, the harvest age 100 days resulted in higher plant growth, indicated by the higher fresh and dry weight of biomass, higher phenol and antioxidant contents than 80 days. Soil mulching, especially with plastic and biodegradable plastic mulches with long harvest age (100 days) effected into lower fresh and dry weight of plants. On the other hand, soil mulching indirectly resulted in lower phenol but higher flavonoid contents through higher soil temperature, while antioxidant contents were higher under the big soil temperature-based Growing Degree Day (GDD). The total phenol, flavonoids and antioxidant produced ranging from 193.75 to 412.50 mg GAE/ 100 g DW; 81.13 to 141.47 mg QE/ 100 g DW; and 1875.5–2144.4 µmol TE/g DW.ConclusionHigher maximum soil temperature resulted in lower phenol content, while higher minimum soil temperature and shorter harvest age increased total flavonoid. Longer harvest age produced more total phenol and antioxidant due to bigger soil temperature-based Growing Degree Day (GDD).Graphical Abstract
Determinants of species assemblages of insect pests in alpine forest ecosystems of western China
Authors: Wang Chun-Jing , Wang Rong , Yu Chun-Mei , Pubu Yongcuo , Sun Wan-Gui , Dang Xiao-Feng , Li Qiang-Feng , Wan Ji-Zhong 
Published: November 2021

AbstractBackgroundInsect pests are a significant threat to natural resources and social development. Modeling species assemblages of insect pests can predict spatiotemporal pest dynamics. However, research gaps remain regarding the mechanism for determining species assemblages of insect pests in alpine forest ecosystems. Here, we explored these determinants using a field investigation conducted for insect pests in a region of the Qinghai-Tibet Plateau. We assessed the species assemblages of insect pests in alpine forest ecosystems based on species co-occurrence patterns and species diversity (i.e., observed diversity, dark diversity, community completeness, and species pool). A probabilistic model was used to test for statistically significant pairwise patterns of species co-occurrence using the presence-absence matrix of pest species based on species interactions. We used ordinary least squares regression modeling to explore relationships between abiotic factors (i.e., climate factors and human influence) and species diversity.ResultsPositive pest species interactions and many association links can occur widely across different investigation sites and parts of plant hosts in alpine forest ecosystems. We detected high dark diversity and low community completeness of insect pests in alpine forest ecosystems. High temperature and precipitation could promote pest species diversity, particularly dark diversity and species pools. Human influence could drive high levels of pest species diversity and lead to dark diversity and species pools. Community completeness could be an effective indicator for insect pest risk assessment.ConclusionsOur study provides new evidence for the determinants of insect pest species assemblages in alpine forest ecosystems from the perspectives of pest species interactions and abiotic factors. The findings of our study could reveal the mechanism for shaping species assemblages and support the prevention and control of insect pests in alpine forest ecosystems.
Object detection for automotive radar point clouds – a comparison
Authors: Scheiner Nicolas , Kraus Florian , Appenrodt Nils , Dickmann Jürgen , Sick Bernhard 
Published: November 2021

AbstractAutomotive radar perception is an integral part of automated driving systems. Radar sensors benefit from their excellent robustness against adverse weather conditions such as snow, fog, or heavy rain. Despite the fact that machine-learning-based object detection is traditionally a camera-based domain, vast progress has been made for lidar sensors, and radar is also catching up. Recently, several new techniques for using machine learning algorithms towards the correct detection and classification of moving road users in automotive radar data have been introduced. However, most of them have not been compared to other methods or require next generation radar sensors which are far more advanced than current conventional automotive sensors. This article makes a thorough comparison of existing and novel radar object detection algorithms with some of the most successful candidates from the image and lidar domain. All experiments are conducted using a conventional automotive radar system. In addition to introducing all architectures, special attention is paid to the necessary point cloud preprocessing for all methods. By assessing all methods on a large and open real world data set, this evaluation provides the first representative algorithm comparison in this domain and outlines future research directions.
Double-objective optimization-based firefly algorithm of a stand-alone photovoltaic/water pumping system for water supply in rural and remote areas: a case study
Authors: Zieba Falama Ruben , Bakari Hamadou , Dumbrava Virgil 
Published: November 2021

AbstractA techno-economic study of a stand-alone PV water pumping system for water supply is done in this paper. An optimal design of the system is realized thanks to a double-objective optimization based on a proposed operational strategy of the system and on firefly algorithm (FA). The two objective functions simulated simultaneously using FA are: the function defining the cost of water (COW) and the function which defines the loss of water supply probability (LWSP). The system is designed to supply water to around 328 households in Cameroon, each composed by an average of six (06) persons. For different LWSP, the double-objective simulation determines the optimal configurations of the system with their related cost. The optimal and reliable (LWSP of 0%) system configuration obtained is composed by a number of 7 PV modules, a reservoir volume of 98.4 m3 with 2 days autonomy, a total dynamic head of 40 m, and a pump power of around 1 kW. The related COW corresponding to this configuration is 0.1185 $/m3, and the total investment cost is 27,506 $. The sensitivity analysis of the system revealed that the PV size, the volume of reservoir storage, and the efficiency of the pump influence the system both technically and economically, whereas the total dynamic head only influences the system economically. The validation of the optimized system has been done by comparison with manufacturer datasheets. It is demonstrated in this paper that the techno-economic analysis of energy systems based on multi-objective optimization using firefly algorithm is a powerful tool for decision-making.
Prevalence of lower limb deformities among primary school students
Authors: Ganeb Sahar Saad , Egaila Sami El-Said , Younis Abdelmoniem Abdelfattah , El-Aziz Asmaa Moheiy Abd , Hashaad Nashwa Ismail 
Published: November 2021

AbstractBackgroundLower limb deformities could affect child's quality of life and may worsen with time. This work aims to study the prevalence of lower limb deformities among primary school students in our governorate.MethodsThis cross-sectional descriptive study was carried out on 4689 students attending 12 public primary schools during the academic year 2019–2020.Complete clinical examination was done for picking of cases of genu varum, genu valgum, genu recurvatum, flat foot, pes cavus, hallux valgus, in-toeing, and lower limb discrepancy, and x-ray on both feet, pelvis, and full-length lower limb was requested.ResultsThis cross-sectional descriptive study included 4689 students. The prevalence of lower limb (LL) deformities was 16.61%. One hundred twenty-three (2.62%) children had a positive history of musculoskeletal pain, 0.09% had genu varum, 0.11% had genu valgum, 0.75% had Genu recurvatum, 0.03% had LL discrepancy, 13.86% had flexible flat foot, 1.22% had rigid flat foot, 0.23% had pes cavus, 1.04 % had in-toeing, 0.06% had hallux varus, and 0.11% had hallux valgus.ConclusionsLower limb deformities are a considerable problem in primary school students that need early diagnosis because it could affect child’s future, health, and career. Further studies are needed to investigate spinal deformity, vit D level, calcium level, foot wear, and school bag weight as hidden factors.