bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional Landscape of Pathogen-responsive Lncrnas Reveals OS_LNC1812-Induced Disease Resistance by Abscisic Acid Pathway in Rice
Authors: Cao Weilin , Gan Liming , Cao Junyuan , wang Ruolin , Li Ying , Gao Jiaqi , Li Changyuan , Zhu Changxiang 
Published: March 2021

Abstract
Background: Long non-coding RNAs (lncRNAs) produced by the plant genome are essential regulators in diverse biological processes. Despite increasing knowledge of lncRNAs in plant development, little is known about responding to plant disease, especially in rice. Results: In this study, we investigated a comprehensive disease-responding lncRNA profiles in rice defence against Rice black-streaked dwarf virus (RBSDV) and Rice stripe virus (RSV) for the first time. Analysis of RNA sequencing of rice leaves infected with the two pathogens identified total 1,925 lncRNAs, in which 724 lncRNA were derived from alternative splicing (AS) events and the largest number of lncRNAs produced by intron retention (IR) of AS events. We calculated the regulatory relationship between transcription factors, lncRNAs, and mRNAs by constructing gene regulatory network and found that a lncRNA, OS_LNC1812 regulated by Os01t0730700-01 encoding transcription factor WRKY14 may target abscisic acid (ABA) responsive element binding factor ABF (Os01t0867300-01) involved in ABA signaling pathway during pathogens infection. We subsequently found that highly expression of OS_LNC1812, WRKY14, and ABF after ABA treatment. Conclusions: Our results reveal the common lncRNAs profiles respond to RBSDV and RSV in rice and provide novel potential modulation of lncRNAs in ABA pathway in the regulation of plant disease resistance.
Uncovering the Multi-level Response of Glycine Max L. To the Application of Allelopathic Biostimulant From Levisticum Officinale Koch
Authors: Szparaga Agnieszka , Kocira Sławomir , Findura Pavol , Kapusta Ireneusz , Zaguła Grzegorz , Świeca Michał 
Published: March 2021

Abstract
The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations. Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch. roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators. Hot method of aqueous extraction was applied. The extracts were administered via foliar application and soil treatment. Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition. The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found. The extracts modified soybean plant physiology, as manifested by changes in biometric traits. Plants responded positively by increased yield. Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content). In addition, they featured changes in their amino acid profile and fatty acid composition. The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins. The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.
Examination of Endobronchial Ultrasound-guided Transbronchial Needle Aspiration Using a Puncture Needle With a Side Trap
Authors: Miyazaki Kazuhito , Hirasawa Yuya , Aga Masaharu , Aiko Naoto , Hamakawa Yusuke , Taniguchi Yuri , Misumi Yuki , Agemi Yoko , Shimokawa Tsuneo , Naoki Katsuhiko , Okamoto Hiroaki 
Published: March 2021

Abstract
Background:Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is useful for diagnosing hilar and mediastinal lymph node enlargement; however, specimens obtained are often small and inadequate for pathologic diagnosis. In June 2017, EchoTip ProCore®, a puncture needle with a side trap, was launched in Japan.Methods:In this single-center prospective interventional study, 57 patients with lymph node or tracheal tumors were diagnosed using EBUS-TBNA with EchoTip ProCore® between June 2017 and February 2020.Results: EBUS-TBNA was performed for 57 patients, 1 had a cyst, 1 had necrotic tissue, and 2 had inadequate specimens; however, the remaining 53 patients had sufficient specimen for histologic diagnosis. The following pathologic subtypes were diagnosed: non-small cell lung cancer, 22; small cell lung cancer, 8; cancer of unknown primary, 2; neuroendocrine tumor (G2) recurrence, 1; lymphoma, 2; metastatic renal cell carcinoma, 3; thymoma recurrence, 1; sarcoidosis, 4; tuberculosis, 1; and non-malignancy, 9. None of the patients had complications. Among 13 patients with inadequate specimens or without malignancy, only one patient was subsequently diagnosed with malignancy, and the median follow-up period was 300 days.Conclusion:EBUS-TBNA using EchoTip ProCore® can obtain a sufficient specimen size for pathologic diagnosis.
Distribution and correlation of methicillin-resistant coagulase-positive staphylococci (MRCoPS) between environmental surfaces, veterinary staff and dogs within a veterinary teaching hospital, Thailand
Authors: Fungwithaya Punpichaya , Murugaiyan Jayaseelan , Prapasarakul Nuvee 
Published: March 2021

Abstract
Background

Coagulase-positive staphylococci (CoPS), a gram-positive cocci bacterium, is a group of bacteria causing dermatitis and septicemia in animal. Methicillin-resistant (MR) CoPS (MRCoPS) can find on human, animal and environmental including medical equipment leading to nosocomial infection. This study aimed to determine the distribution and to analyse correlation of methicillin-resistant coagulase-positive staphylococci (MRCoPS) between environmental surfaces, veterinary staff, and dogs within a Veterinary Teaching Hospital (VTH) at Chulalongkorn University (CU) in Bangkok, Thailand. All isolates were characterized the antimicrobial susceptibilities, Staphylococcal cassette mec (SCCmec) typing and clone typing by Pulsed-Field Gel Electrophoresis (PFGE).
Results

In total, 88 CoPS isolates were obtained from 53 samples comprising 24 parts of floors (24/53, 45.3%), 14 items of medical instruments (14/53, 26.4%), 14 dogs (14/53, 26.4%) and a veterinarian (1/53, 1.9%). Sixty-two of 88 were MRCoPS locating in the five hospital rooms. The highest MRCoPS species were MR Staphylococcus pseudointermedius (MRSP) isolates (57/62, 91.9%) retrieved from 26 samples and mainly discovered on the floors. Seven antibiogram patterns and two SCCmec types were resolved from 62 MRCoPS isolates. Most of the MRCoPS (93.6%) were resistant to at least three antibiotics. All MR Staphylococcus schleiferi subsp. coagulans (MRSSc) isolates displayed a triple antibiogram associated with the SCCmec type V, while the MRSP isolates showed various antibiograms and SCCmec types. The S. pseudintermedius in dogs and three rooms were related by their PFGE pattern A. The average percentage of MRCoPS-positive surfaces were typically high on the floors (20%). The dermatological room showed the highest of MRCoPS-positive surfaces on both the floor and medical instruments.
Conclusions

The distribution of CoPS and MRCoPS has become a relative common situation in veterinary hospitals, and so attention must be paid to limit cross-contamination between patients and the hospital environment. To general; please provide impact of this study digested as a take home message.
The Impact of Frailty on Survival in Elderly Intensive Care Patients with COVID-19 – the COVIP Study
Authors: Jung Christian , Flaatten Hans , Fjølner Jesper , Bruno Raphael Romano , Wernly Bernhard , Artigas Antonio , Pinto Bernardo Bollen , Schefold Joerg C. , Wolff Georg , Kelm Malte , Beil Michael , Sigal Sviri , Heerden Peter Vernon van , Szczeklik Wojciech , Czuczwar Miroslaw , Elhadi Muhammed , Joannidis Michael , Oeyen Sandra , Zafeiridis Tilemachos , Marsh Brian , Andersen Finn H. , Moreno Rui , Cecconi Maurizio , Leaver Susannah , Boumendil Ariane , De Lange Dylan W. , Guidet Bertrand 
Published: March 2021

Abstract
BackgroundThe COVID-19 pandemic has led highly developed healthcare systems to the brink of collapse due to the large numbers of patients being admitted into hospitals. One of the potential prognostic indicators in patients with COVID-19 is frailty. The degree of frailty could be used to assist both the triage into intensive care, and decisions regarding treatment limitations. Our study sought to determine the interaction of frailty and age in elderly COVID-19 ICU patients.MethodsA prospective multi-centre study of COVID-19 patients ≥ 70 years admitted to intensive care in 138 ICUs from 28 countries was conducted. The primary endpoint was 30-day mortality. Frailty was assessed using the Clinical Frailty Scale (CFS). Additionally, comorbidities, management strategies and treatment limitations were recorded.ResultsThe study included 1346 patients (28% female) with a median age of 75 years (IQR 72-78, range 70-96), 16.3% were older than 80 years and 21% of the patients were frail. The overall survival at 30 days was 59% (95%CI 56-62), with 66% (63-69) in fit, 53% (47-61) in vulnerable and 41% (35-47) in frail patients (p<0.001). In frail patients, there was no difference in 30 day survival between different age categories. Frailty was linked to an increased use of treatment limitations and less use of mechanical ventilation. In a model controlling for age, disease severity, sex, treatment limitations and comorbidities, frailty was independently associated with lower survival.ConclusionFrailty provides relevant prognostic information in elderly COVID-19 patients in addition to age and comorbidities.
Identification of A New Kind of Phenazine Compounds Attenuating The Stemness of Breast Cancer Cells Through Triggering Ferroptosis
Authors: Yang Yue , Lu yuanyuan , Zhang Chunhua , Guo Qianqian , Wang Ting , Xia Zhuolu , Liu Jing , Cheng Xiangyu , Xi Tao , Jiang Feng , zheng lufeng 
Published: March 2021

Abstract
Background: Breast cancer stem cells (BCSCs) are positively correlated with metastasis, chemoresistance and recurrence of breast cancer.Methods: The stemness were analyzed by qPCR and western blot, flow cytometry, cell spheroid formation assay, and tumor xenograft model. The cell viability was performed by MTT. The transcriptome analysis was used to evaluate the influence of these three compounds. The migration was analyzed by wound-healing assay, transwell invasion analysis, and metastasis model. The iron concentration was analyzed by fluorescence microscopy analysis. The lipid peroxidation and ROS level were analyzed by flow cytometry. The results were presented as mean ± SD, the analysis was Student’s t-test by using GraphPad Prism 5 software. P-values less than 0.05 were considered to be statistically significant. (*p < 0.05, **p < 0.01, ***p < 0.001).Results: Here, we tried to screen out small-molecule compounds targeting BCSCs from the phenazine library established by us before. We focused on the compounds without affecting cell viability and screened out three potential compounds (CPUL119, CPUL129, CPUL149) that can significantly attenuate the stemness of breast cancer cells, as evident by the decrease of stemness marker expression, CD44+/CD24- subpopulation, mammary spheroid-formation ability and tumor-initiating capacity. Additionally, these compounds suppressed the metastatic ability of breast cancer cells in vitro and in vivo. Combined with the transcriptome-sequencing analysis in breast cancer cells with or without the treatment of these three compounds, respectively, it was found that ferroptosis was shown on the top of the most upregulated pathways by CPUL119, CPUL129 and CPUL149. Mechanistically, we found that CPUL119, CPUL129 and CPUL149 could trigger ferroptosis by accumulating and sequestering iron in lysosomes through interacting with iron, and by regulating the expression of proteins (IRP2, TfR1, ferritin) engaged in modulating iron transport and storage. Furthermore, inhibition of ferroptosis rescued the suppression of these three compounds on the stemness of breast cancer cells. Conclusions: This study suggests that CPUL119, CPUL129 and CPUL149 can specifically inhibit the stemness of breast cancer cells through ferroptosis and may be the potential compounds for breast cancer treatment.
Redox Index of Cys-Thiol Residues of Serum Apolipoprotein E and Its Diagnostic Potential
Authors: Yamauchi Kazuyoshi , Kawakami Yasushi 
Published: March 2021

Abstract
We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named “redox-IDX-apoE,” which is necessary to understand the redox biology of age-related diseases, such as atherosclerosis. The fractions of reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE were measured by a band-shift assay. Candidates of redox-IDX-apoE were determined by calculating the values of these fractions and total apoE concentration. The ratios of roxi-apoE to total-apoE (roxi/total), red-apoE to roxi-apoE (red/roxi), and [red-apoE + oxi-apoE] to roxi-apoE ([red + oxi]/roxi) were independent of age and sex. Roxi/total showed significant negative correlations with serum triglyceride (TG) and HbA1c levels, while red/roxi and [red + oxi]/roxi showed significant positive correlations with them. However, red/roxi and [red + oxi]/roxi in the patients with atherosclerosis were significantly lower than those in control subjects, although serum TG and HbA1c levels in the patients were significantly higher than those in controls. The redox status of serum apoE-Cys-thiol is closely involved in the metabolism of TG-rich lipoproteins and glucose and may vary depending on the difference in pathological conditions. The appropriate usage of these ratios could be helpful in the diagnosis and prognosis of age-related diseases.
Unique and Combined Contribution of Peer Victimization and Maltreatment in Childhood to Young Adults’ Anxiety, Depression, and Suicidality: A Cross-Sectional Study
Authors: Macalli Melissa , Orri Massimiliano , Tzourio Christophe , Côté Sylvana M. 
Published: March 2021

Abstract
BackgroundChild maltreatment and peer victimization are known to be major risk factors for depression and suicidal behavior. Furthermore, child maltreatment increases the risk for victimization by peers. Our objective was to distinguish the contribution of maltreatment by parents and peer victimization to mental health problems in young adulthood. Specifically, we tested whether peer victimization alone or in combination with parental maltreatment before 18 years was associated with anxiety, depression, and suicidal thoughts and behaviors at age 21 years.MethodsWe used data collected in the i-Share (internet-based students’ health research enterprise) study in France from February 2013 to September 2019 (N = 2271 participants). We conducted multinomial and binary logistic regression analyses to assess the unique and cumulative contribution of peer victimization and parental maltreatment with anxiety, depression, and suicidality. ResultsAlmost one third of students (28.8%) reported at least one mental health problem; 29.8% reported peer victimization only; 7.5% reported parental maltreatment only; and 10.3% reported both maltreatment and victimization. In multivariate models, compared to participants who did not experience maltreatment or peer victimization, those who experienced peer victimization only were more likely to present anxiety (aOR: 1.90; 95% CI: 1.50-2.40), depression (aOR: 1.95; 95% CI: 1.46-2.60), or suicidal ideation without (aOR: 1.62; 95% CI: 1.26-2.09) and with attempt (aOR: 2.70; 95% CI: 1.51-4.85). Similar associations were observed for those who were maltreated only. Those experiencing both maltreatment and peer victimization were at increased risk for depression (aOR: 2.63; 95% CI: 1.79-3.86) and for suicidal ideation with (aOR: 9.19; 95% CI: 4.98-16.92) and without attempt (aOR: 2.64; 95% CI: 1.86-3.76).ConclusionsIndividual and combined exposure to parental maltreatment and peer victimization in childhood or adolescence was associated with increased risk for anxiety, depression and suicidal behaviors. Peer victimization seems to have a specific role on mental health disorders not otherwise explained by polyvictimization. Since peer victimization is a frequent and evitable child abuse type, the findings have implications for policies to prevent and deal with.
Determinants of Necrotizing Enterocolitis Among Neonates in Referral Hospitals of East and West Gojjam Zones of Amhara Regional State, Northwest Ethiopia, 2020.
Authors: Derbew Wondim , Dagnew Amare , Girma Frehiwot 
Published: March 2021

Abstract
Background: Necrotizing enterocolitis is one of the most common serious surgical disorders of neonates. It is a life-threatening emergency of the gastrointestinal tract in the neonatal period that causes morbidity and mortality of newborns. Although the burden of the disease is high and it is the major cause for neonatal death, there is limited information’s about identifying the determinant factors that may help to reduce the neonatal deaths. Thus this study aimed to identify the determinants of necrotizing enterocolitis among admitted neonates in neonatal intensive care unit at referral hospitals of East and West Gojjam Zones of Amhara regional state,Northwest Ethiopia, 2020. Methods: Unmatched case control study design was conducted among 246 neonates (82 cases and 164 controls) in neonatal intensive care unit from February 24 to April 24, 2020. Data was collected through face to face interview and reviewing medical charts of the neonates. Data was entered into Epi data and exported to SPSS for analysis. Bivariable logistic regression analysis was used to identify the candidate variables at p≤0.25 and multivariable logistic regression analysis was employed to identify significant determinants at p value <0.05. Adjusted odds ratio with 95% CI was used to show the strength of association between exposure and outcome variables. Result: A total of 246 neonates were included in the study. Duration of rupture of membrane>18 hours [AOR=4.287; 95%CI (2.157-8.518)], low birth weight [AOR= 3.592; 95% CI (1.742- 7.407)], neonatal sepsis [AOR=3.553; 95% CI (1.76-7.174)] and types of enteral feeding (formula milk only [AOR=3.604; 95% CI (1.548-8.39)] and mixed milk (AOR=2.416; 95% CI (1.103-5.290)]) were determinants for necrotizing enterocolitis.Conclusions: Duration of rupture of membrane, low birth weight, neonatal sepsis and enteral feedings (formula milk and mixed milk) were significantly associated with necrotizing enterocolitis. Encouraging exclusive breastfeeding, providing adequate care for low birth weight babies and using septic precautions to avoid neonatal sepsis are the strategies to prevent necrotizing enterocolitis.
Disruptive Impacts of Living With Chronic Low Back Pain and Experience of&nbsp;Psychologically Informed Physical Therapy – A Qualitative Process Evaluation of a Randomised Control Trial
Authors: Petrozzi Marco John , Spencer Grace , Mackey Martin G 
Published: March 2021

Abstract
Background: Chronic low back pain (LBP) is a significant and complex health condition affecting one in ten people worldwide. Research has shown that LBP can negatively impact the physical, psychological and social aspects of people’s lives. Clinical practice guidelines recommend a combined physical and psychological management approach (psychologically informed physical therapy) for chronic LBP. A recent multi-site randomised controlled trial (Mind Your Back) aimed to investigate whether combining multimodal physical treatments with an internet-delivered psychosocial intervention (MoodGYM) was more effective for improving disability and self-efficacy in people with chronic LBP, compared to standard treatment. The aim of this study was to conduct a process evaluation to explore the participants’ experiences of living with chronic LBP and their response to interventions provided in the Mind Your Back Trial.Methods: Twenty-five participants volunteered to take part in a semi-structured telephone interview about their experiences of taking part in the Mind Your Back trial and to understand their perspectives of living with chronic back pain. Interviews were transcribed verbatim and data analysed thematically. Results: Three main themes were identified: (1) Ongoing back pain disrupts all aspects of life, (2) Personalised support and therapeutic alliance are important, and (3) MoodGYM lacked relevant, personalised and tailored support. Conclusion: Living with chronic LBP has significant disruptive impacts on everyday life. It is crucial to deliver tailored support and management strategies that are grounded in the everyday lived experiences of people with chronic LBP.
How well do genetic markers inform about responses to intraspecific admixture? A comparative analysis of microsatellites and RADseq
Authors: Yıldırım Yeşerin , Forsman Anders , Sunde Johanna 
Published: March 2021

Abstract
Background: Fitness consequences of intraspecific genetic admixture can vary from positive to negative depending on the genetic composition of the populations and environmental conditions. Because admixture has potential to influence the success of management and conservation efforts, genetic similarity has been suggested to be used as a proxy to predict the outcome. Studies utilizing microsatellites (a neutral marker) to investigate associations between genetic distance and admixture effects show conflicting results. Marker types that yield information on genome-wide and/or adaptive variation might be more useful for predicting responses to inter-population hybridization. In this study we utilized published data for three populations of pike (Esox lucius) to investigate associations between offspring performance (hatching success) and parental genetic similarity in experimentally purebred and admixed families, based on neutral (microsatellites), genome-wide neutral (RADseq SNPs), and adaptive (SNPs under selection) markers.Results: Estimated similarity varied among the markers, likely reflecting differences in their inherent properties, but was consistently higher in purebred than admixed families. A significant interaction between marker type and admixture treatment reflected that neutral SNPs yielded higher estimates than adaptive SNPs for admixed families whereas no difference was found for purebred families, which indicates that neutral similarity was not reflective of adaptive similarity. When all samples were pooled, no association between similarity and performance was found for any marker. For microsatellites, similarity was positively correlated with hatching success in purebred families, whereas no association was found in admixed families; however, the direction of the effect differed between the population combinations. Conclusions: The results strengthen the notion that, as of today, there is no proxy that can reliably predicted the outcome of admixture. This emphasizes the need of further studies to advance knowledge that can shed light on how to safeguard against negative consequences of admixture, and thereby inform management and promote conservation of biological diversity.