bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishing a Novel Treatment Algorithm for Pediatric Mandibular Tumor Reconstruction
Authors: Chan Christopher , Włodarczyk Jordan R. , Wolfswinkel ErikM. , Odono Lauren T. , Urata Mark M. , Hammoudeh Jeffrey A. 
Published: October 2021

Introduction:
Guidelines for pediatric mandibular reconstruction (PMR) are not well-established. One must consider the growing craniofacial skeleton, mixed dentition, long-term dental occlusion, need for secondary reconstruction, and speech development. The traditional guideline (bone defect > 5 cm) for use of vascularized bone grafts (VBG) is not applicable given the variation of pediatric mandibular size and growth. We seek to propose a novel algorithm for PMR.

Materials and Methods:
An Institutional Review Board approved retrospective review of patients who underwent PMR for tumor resections between 2005 and 2019 evaluated patients’ demographics, complications, resection index (RI) (resection length to mandibular length), and surgical outcomes. Outcomes based on RI were analyzed to establish guidelines for VBG utilization.

Results:
Twenty-four patients underwent PMR at a mean age of 9.1 years (range: 1 – 18). The mandibular defect (mean± standard deviation) fornon-VBG (n
= 18) and VBG (n
= 6) was 6.6 ± 3.0 cm and 12.8 ± 4.3 cm, respectively. The VBG group had fewer return trips to the operating room (P
= 0.028) and fewer major complications (P = 0.028). When non-VBG with RI > 32% were compared to <32%, there was statistically less returns to the operating room for complications and a lower rate of early (< 30 days) major complications.

Discussion:
Our algorithm proposes an RI cutoff of 32% for VBG use for PMR. Patients with a sizable soft tissue defect, previous chemotherapy and/or radiation, planned adjuvant chemotherapy and/or radiation therapy, or a history of failed non-VBG should undergo reconstruction using VBG.
Remote patient monitoring to interrupt chains of respiratory infections in outpatient care - a case-control study during the 2020/21 infection season
Authors: Eichler Sarah , Carnarius Sebastian , Steiger Edgar , von Stillfried Dominik 
Published: October 2021

AbstractAim of the studyThe aim of the study was to investigate satisfaction, saving of time and the possible reduction of patient visits to practices that use Remote Patient Monitoring (RPM) during treatment compared to usual care.MethodsIn a case-control study between October 2020 and May 2021, the participating practices were randomized into three groups (two different RPM systems, one control). The doctors were required to enroll patients with acute respiratory infection ≥ 18 years who have a web-enabled device. After a three-month study phase, the doctors were asked to describe the treatment of their patients via online survey. The patients were also questioned. The analysis was carried out descriptively and with group comparisons.Results51 practices with 121 patients were included. Overall, the results show a positive assessment of digital care on the patient side. As for the doctors, handling and integration of the systems into consisting practice processes seem to be a challenge. Further, the number of patient visits to the practice was not reduced by using the systems and the doctors did not save time, but the relationship to the patients was intensified.ConclusionEven if there were no indications for more efficiency by using the RPM systems, the doctors see great potential to intensify the interaction between doctor and patient. In particular, more intensive contact with patients with chronic diseases (e. g. COPD, long-COVID) could be of long-term interest and importance for doctors in outpatient care.Trial RegistrationDRKS00023553
A molecular surveillance-guided vector control response to concurrent dengue and West Nile virus outbreaks in a COVID-19 hotspot of Florida
Authors: Coatsworth Heather , Lippi Catherine A. , Vasquez Chalmers , Ayers Jasmine B. , Stephenson Caroline J. , Waits Christy , Florez Mary , Wilke André B. B. , Unlu Isik , Medina Johana , Alcaide Maria L. , Ryan Sadie J. , Lednicky John A. , Beier John C. , Petrie William , Dinglasan Rhoel R. 
Published: October 2021

AbstractSimultaneous dengue virus (DENV) and West Nile virus (WNV) outbreaks in Florida, USA, in 2020 resulted in 71 dengue virus serotype 1 and 86 WNV human cases. Our outbreak response leveraged a molecular diagnostic screen of mosquito populations for DENV and WNV in Miami-Dade County to quickly employ targeted mosquito abatement efforts. We detected DENV serotypes 2 and 4 in mosquito pools, highlighting the silent circulation of diverse dengue serotypes in mosquitoes. Additionally, we found WNV-positive mosquito pools in areas with no historical reports of WNV transmission. These findings demonstrate the importance of proactive, strategic arbovirus surveillance in mosquito populations to prevent and control outbreaks, particularly when other illnesses (e.g., COVID-19), which present with similar symptoms are circulating concurrently. Growing evidence for substantial infection prevalence of dengue in competent mosquito vectors in the absence of local index cases suggests a higher level of dengue endemicity in Florida than previously thought.Article Summary LineEvidence of increasing dengue endemicity in Florida: Vector surveillance during dengue and West Nile virus outbreaks revealed widespread presence of other dengue virus serotypes in the absence of local index cases.
Fetal cerebellar development: 3D morphometric analysis of fetal postmortem MRI among Chinese fetuses
Authors: Suluba Emmanuel , Shuwei Liu 
Published: October 2021

AbstractThe development of the cerebellum starts from early gestational period and extends postnatal. Because of its protracted development, the cerebellum is susceptible to developmental anomalies such as Dandy-Walker malformations, Blake’s pouch cysts and vermin hypoplasia. Measurements of fetal cerebellar parameters of a normal growing fetus in each week of gestation is important for setting up morphometric standards and hence used as clinical reference data. Any deviation from the normal cerebellar parameters alerts the clinicians for the possibility of presence of cerebellar malformations.Study objectiveThe objective of this study was to assess the fetal cerebellar growth by quantifying the following parameters; fetal cerebellar volume, anterior-posterior diameter and superior-inferior diameter.MethodsWe used 3T and 7T MRI to scan the postmortem fetal brains at different stages of development and subsequently analyze the images using ITK-SNAP software.ResultsThe mean superior-inferior cerebellar diameter was found to be 19.12±2. 70mm.The linear(y=bo+b1t) model was the best fit (r2=0.996, F=32022.961) to describe the relationship between the gestational age and the superior-inferior diameter(y=5.89+0.49t). There was significant correlation between the superior-inferior cerebellar diameter and the gestation age, Pearson correlation coefficient of 0.999, r=0.001. The median cerebellar volume was 8607.7mm, the mean rank high among males(78.12) as compared to female(68.25).There was no statistically significant difference of the cerebellar volume between males and females (u=2193.5,p=0.16).The quadratic(y=bo+b1t+b2t2) model was the best fit regression equation (r2=0.994,F=10791.157) describing the relationship between the cerebellar volume and the gestational age. The median anterior- posterior diameter was 12.45 mm. There was significant correlation between anterior-posterior diameter and the gestational age with Spearman’s rho of (0.997, p=0.01). The linear model was the best fit the best fit model (y=bo+b1t) describing the relationship between anterior-posterior diameter and the gestational age(y=3.31+0.5t) r2=0.998, F=70646.838ConclusionSignificant correlation between the superior-inferior cerebellar diameters, the anterior-posterior cerebellar diameter and the gestational age was found. These two linear parameters follow the first-degree polynomial in relation to the gestational age. The cerebellar volume follows the second-degree polynomial as it increases with the gestational age and correlate significantly with the gestational age. This study has provided new insight to the development of the cerebellum, and setup a benchmark data of which the deviation from it will alert the clinicians for the possibility of presence of cerebellar malformations.
Mutating novel interaction sites in NRP1 reduces SARS-CoV-2 spike protein internalization
Authors: Pal Debjani , De Kuntal , Yates Timothy B. , Muchero Wellington 
Published: October 2021

AbstractThe global pandemic of Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 has become a severe global health problem because of its rapid spread(1). Both angiotensin-converting enzyme 2 and neuropilin 1 provide initial viral binding sites for SARS-CoV-2 (2, 3). Here, we show that three cysteine residues located in a1/a2 and b1 domains of neuropilin 1 are necessary for SARS-CoV-2 spike protein internalization in human cells. Mutating cysteines C82, C104 and C147 altered neuropilin 1 stability and binding ability as well as cellular internalization and lysosomal translocation of the spike protein. This resulted in up to 4 times reduction in spike protein load in cells for the original, alpha and delta SARS-CoV-2 variants even in the presence of the endogenous angiotensin-converting enzyme 2 receptor. Transcriptome analysis of cells transfected with mutated NRP1 revealed significantly reduced expression of genes involved in viral infection and replication, including eight members of the ribosomal protein L, ten members of ribosomal protein S and five members of the proteasome β subunit family proteins. We also observed higher expression of genes involved in suppression of inflammation and endoplasmic reticulum associated degradation. These observations suggest that these cysteines offer viable targets for therapies against COVID-19.
Secondary structure of subgenomic RNA M of SARS-CoV-2
Authors: Soszynska-Jozwiak Marta , Kierzek Ryszard , Kierzek Elzbieta 
Published: October 2021

AbstractSARS-CoV-2 belongs the Coronavirinae family. As other coronaviruses, SARS-CoV-2 is enveloped and possesses positive-sense, single-stranded RNA genome of ∼ 30 kb. Genome RNA is used as the template for replication and transcription. During these processes, positive-sense genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are created. Several studies showed importance of genomic RNA secondary structure in SARS-CoV-2 replication. However, the structure of sgRNAs have remained largely unsolved so far. In this study, we performed probing of sgRNA M of SARS-CoV-2 in vitro. This is the first experimentally informed secondary structure model of sgRNA M, which presents features likely to be important in sgRNA M function. The knowledge about sgRNA M provides insights to better understand virus biology and could be used for designing new therapeutics.
Thalamic activity during scalp slow waves in humans
Authors: Ujma Péter P. , Szalárdy Orsolya , Fabó Dániel , Erőss Loránd , Bódizs Róbert 
Published: October 2021

AbstractSlow waves are major pacemakers of NREM sleep oscillations. While slow waves themselves are mainly generated by cortical neurons, it is not clear what role thalamic activity plays in the generation of some oscillations grouped by slow waves, and to what extent thalamic activity during slow waves is itself driven by corticothalamic inputs. To address this question, we simultaneously recorded both scalp EEG and local field potentials from six thalamic nuclei (bilateral anterior, mediodorsal and ventral anterior) in fifteen epileptic patients (age-range: 17-64 years, 7 females) undergoing Deep Brain Stimulation Protocol and assessed the temporal evolution of thalamic activity relative to scalp slow waves using time-frequency analysis. We found that thalamic activity in all six nuclei during scalp slow waves is highly similar to what is observed on the scalp itself. Slow wave downstates are characterized by delta, theta and alpha activity and followed by beta, high sigma and low sigma activity during subsequent upstates. Gamma activity in the thalamus is not significantly grouped by slow waves. Theta and alpha activity appeared first on the scalp, but sigma activity appeared first in the thalamus. These effects were largely independent from the scalp region in which SWs were detected and the precise identity of thalamic nuclei. Our results indicate that while small thalamocortical neuron assemblies may initiate cortical oscillations, especially in the sleep spindle range, the large-scale neuronal activity in the thalamus which is detected by field potentials is principally driven by global cortical activity, and thus it is highly similar to what is observed on the scalp.
MicroPET evidence for a hypersensitive neuroinflammatory profile of gp120 mouse model of HIV
Authors: Young Jared W. , Barback Christopher V. , Stolz Louise A. , Groman Stephanie M. , Vera David R. , Hoh Carl , Kotta Kishore K. , Minassian Arpi , Powell Susan B. , Brody Arthur L. 
Published: October 2021

AbstractDespite increased survivability for people living with HIV (PLWH), HIV-related cognitive and behavioral abnormalities persist. Determining the biological mechanism(s) underlying these abnormalities is critical to minimize the long-term impact of HIV. Human positron emission tomography (PET) studies reveal that PLWH exhibit higher neuroinflammation, which may contribute to cognitive and behavioral problems. PLWH are hypersensitive to environmental insults that drive elevated inflammatory profiles. Gp120 is an envelope glycoprotein exposed on the surface of the HIV envelope which enables HIV virus entry into a cell and contributes to HIV-related neurotoxicity. Gp120 overexpression in mice enables delineating its impact, including on neuroinflammation. In vivoevidence for gp120 transgenic (Tg) mice exhibiting neuroinflammation has yet to be determined.Here, we conducted microPET imaging in male gp120 Tg and wildtype mice, using the radiotracer [(18)F]FEPPA which binds to the translocator protein expressed by activated microglial and serves as a marker of neuroinflammation. Imaging was performed at baseline and 24 hours after treatment with lipopolysaccharide (LPS; 5 mg/kg), endotoxin that triggers an immune response.Gp120 Tg mice exhibited elevated [(18F)]FEPPA in response to LPS vs. wildtype mice throughout the brain including dorsal and ventral striata, hypothalamus, and hippocampus, but not prefrontal cortex.Gp120 Tg mice are hypersensitive to environmental inflammatory insults, consistent with PLWH, measurablein vivo. It remains to-be-determined whether this heightened sensitivity is connected to the behavioral abnormalities of these mice or is sensitive to antiretroviral or other treatments.
Forward simulations of walking on a variable surface-impedance treadmill: A comparison of two methods
Authors: Abdikadirova Banu , Price Mark , Hoogkamer Wouter , Huber Meghan E. 
Published: October 2021

AbstractRecent experiments with a variable stiffness tread-mill (VST) suggest that modulating foot-ground contact dynamics during walking may offer an effective new paradigm for gait rehabilitation. How gait adapts to extended perturbations of asymmetrical surface stiffness is still an open question. In this study, we simulated human gait with prolonged asymmetrical changes in ground stiffness using two methods: (1) forward simulation of a muscle-reflex model and (2) optimal control via direct collocation. Simulation results showed that both models could competently describe the biomechanical trends observed in human experiments with a VST which altered the walking surface stiffness for one step. In addition, the simulations revealed important considerations for future experiments studying the effect of asymmetric ground stiffness on gait behavior. With the muscle-reflex model, we observed that although subtle, there was a difference between gait biomechanics before and after the prolonged asymmetric stiffness perturbation, showing the behavioral signature of an aftereffect despite the lack of supraspinal control in the model. In addition, the optimal control simulations showed that damping has a large effect on the overall lower-body muscle activity, with the muscle effort cost function used to optimize the biomechanics increasing 203% between 5 Ns/m and 2000 Ns/m at a stiffness of 10 kN/m. Overall, these findings point to new insights and considerations for advancing our understanding of human neuromotor control of locomotion and enhancing robot-aided gait rehabilitation.
A bacterial Argonaute with efficient DNA and RNA cleavage activity guided by small DNA and RNA
Authors: Wang Longyu , Xie Xiaochen , Liu Yang , Li Wenqiang , Lv Bin , Zhang Zhiwei , Yang Jun , Yan Guangbo , Chen Wanping , Zhang Cheng , Wang Fei , Ma Lixin 
Published: October 2021

ABSTRACTArgonaute proteins are widespread in prokaryotes and eukaryotes. Most prokaryotic Argonaute proteins (pAgos) use 5’P-gDNA to target complementary DNA. However, more and more studies on the properties of pAgos make their functions more diversified. Previously reported pAgos only possess several forms of high activity in all eight cleavage patterns, which limits their practical applications. Here, we described a unique pAgo from Marinitoga hydrogenitolerans (MhAgo) with eight cleavage activities. MhAgo can utilize all four types of guides (5’OH-gDNA, 5’P-gDNA, 5’OH-gRNA, and 5’P-gRNA) for ssDNA and RNA cleavage. Further studies demonstrated that MhAgo had high activities with 16-21 nt guides and no obvious preferences for the 5’-end nucleotides of 5’OH-guides. Unexpectedly, MhAgo had different preferences for the 5’-end nucleotides of 5’P-guides depending on the types of targets. Although the specificity of MhAgo was related to the types of guides, single mismatches in the central and 3’-supplementary regions of guides greatly reduced the cleavage efficiency. Additionally, the electrophoretic mobility shift assay (EMSA) demonstrated MhAgo had the weakest affinity for 5’P-gRNA:tRNA duplex, which was consistent with its cleavage efficiency. In conclusion, MhAgo is highly active under a wide range of conditions and can be used for programmable endonucleolytic cleavage of both ssDNA and RNA substrates. The abundant biochemical characteristics of MhAgo broaden our understanding of pAgos and expand the potential application in nucleic acids manipulations.
Accounting and gender equality in (times of) crisis: toward an accounting that accommodates for emotional work?
Authors: Perray-Redslob Ludivine , Younes Dima 
Published: October 2021

PurposeEmpirically, this paper questions whether accounting can help cope with crisis and preserve some form of feminist ideals. Theoretically, this paper aims to explore how accounting affects the division of emotional work in times of crisis.Design/methodology/approachThe paper relies on a qualitative study that investigates “life under lockdown” during the COVID crisis and focuses on middle-class well-educated couples aspiring for some form of gender equality and who introduced accounting tools (schedules, charts, to-do-lists, etc.) in their daily life to achieve it.FindingsThe paper argues that accounting tools are not able to prevent couples from adopting traditional ways of carrying out emotional work. By favoring the masculine way of displaying emotions, they make invisible women's efforts for comforting. They even mask the unequal distribution of emotional work under some form of “neoliberal equality”. Also, in a context where middle-class standards are perceived as crucial to meet for both parents to keep their social position, accounting tools, by holding parents accountable for these standards, let no time to find alternative ways of living. Consequently, traditional roles become impossible to reverse.Research limitations/implicationsThe paper investigates accounting, gender and emotions by showing the importance of making emotional work visible at a household but also at an organizational and societal level. It calls for an “integrative” emotional display that is crucial for resilience in times of crisis and invites to challenge neoliberal middle-class standards that make household life difficult for most women. Theoretically, it invites for further exploring how accounting tools are constructed and negotiated and how unpredictable elements of life other than emotions affect gender when accounting tools are introduced in times of crisis.Originality/valueThis article contributes to the literature on gender-in-accounting by introducing the concept of emotional work and showing how accounting tools affect the gendered division of emotional work in praxis.
Developing an extended model of self-congruity to predict Chinese tourists' revisit intentions to New Zealand: the moderating role of gender
Authors: Yang Shaohua , Isa Salmi Mohd , Ramayah T. , Wen Jun , Goh Edmund 
Published: October 2021

PurposeThis study developed an extended model of self-congruity by integrating destination image, destination personality, self-congruity, revisit intention and gender.Design/methodology/approachSurveys were conducted with 645 Chinese tourists visiting New Zealand. Partial least squares structural equation modelling (PLS-SEM) was performed to estimate linkages between destination image, destination personality, self-congruity and revisit intention. To compare effects on revisit intention across male and female Chinese tourists, PLS-Henseler's multigroup analysis and PLS-permutation test were conducted to analyse gender as a moderator in the proposed framework.FindingsOur results revealed positive direct effects among destination image, destination personality, self-congruity and revisit intention. Our findings indicated a highly significant difference in the effects of destination personality on ideal self-congruity across male and female Chinese tourists. The association between destination image and self-congruity identified through this model represents a crucial contribution to the tourism literature. This study also enriches tourism research by comparing male and female Chinese tourists' intentions to revisit New Zealand, having identified crucial heterogeneity within female tourists.Practical implicationsThe practical implications from our research can improve destination marketing organization (DMO) officials' awareness of one-time and repeat Chinese tourists' experiences, which strongly trigger subsequent visits.Originality/valueThis paper is the first to examine the direct correlations among destination image, destination personality, self-congruity and revisit intention by considering whether gender might moderate these factors. Our study innovatively adopted PLS-SEM along with several advanced analytical approaches, such as multigroup analysis (MGA) of women and men, to examine our research model.