bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cucumber powdery mildew detection using hyperspectral data
Authors: Fernández Claudio I. , Leblon Brigitte , Wang Jinfei , Haddadi Ata , Wang Keri 
Published: November 2021

This study aimed to understand the spectral changes induced by Podosphaera xanthii, the causal agent of powdery mildew, in cucumber leaves from the moment of inoculation until visible symptoms are apparent. A principal component analysis (PCA) was applied to the spectra to assess the spectral separability between healthy and infected leaves. A spectral ratio between infected and healthy leaf spectra was used to determine the best wavelengths for detecting the disease. Additionally, the spectra were used to compute two spectral variables [i.e., the red-well point (RWP) and the red-edge inflexion point (REP)]. A linear support vector machine (SVM) classifier was applied to certain spectral features to assess how well these features can separate the infected leaves from the healthy ones. The PCA showed that a good separability could be achieved from 4 days post-inoculation (DPI). The best model to fit the RWP and REP wavelengths corresponded to a linear model. The linear model had a higher adjusted R2 for the infected leaves than for the healthy leaves. The SVM trained with five first principal components scores achieved an overall accuracy of 95% at 4 DPI (i.e., two days before the visible symptoms). With the RWP and REP parameters, the SVM accuracy increased as a function of the day of inoculation, reaching 89% and 86%, respectively, when symptoms were visible at 6 DPI. Further research must consider a higher number of samples and more temporal repetitions of the experiment.
Exquisite Sensitivity to Dual BRG1/BRM ATPase Inhibitors Reveals Broad SWI/SNF Dependencies in Acute Myeloid Leukemia
Authors: Rago Florencia , Rodrigues Lindsey Ulkus , Bonney Megan , Sprouffske Kathleen , Kurth Esther , Elliott GiNell , Ambrose Jessi , Aspesi Peter , Oborski Justin , Chen Julie T. , McDonald E. Robert , Mapa Felipa A. , Ruddy David A. , Kauffmann Audrey , Abrams Tinya , Bhang Hyo-eun C. , Jagani Zainab 
Published: November 2021

Abstract

Various subunits of mammalian SWI/SNF chromatin remodeling complexes display loss-of-function mutations characteristic of tumor suppressors in different cancers, but an additional role for SWI/SNF supporting cell survival in distinct cancer contexts is emerging. In particular, genetic dependence on the catalytic subunit BRG1/SMARCA4 has been observed in acute myelogenous leukemia (AML), yet the feasibility of direct therapeutic targeting of SWI/SNF catalytic activity in leukemia remains unknown. Here, we evaluated the activity of dual BRG1/BRM ATPase inhibitors across a genetically diverse panel of cancer cell lines and observed that hematopoietic cancer cell lines were among the most sensitive compared with other lineages. This result was striking in comparison with data from pooled short hairpin RNA screens, which showed that only a subset of leukemia cell lines display sensitivity to BRG1 knockdown. We demonstrate that combined genetic knockdown of BRG1 and BRM is required to recapitulate the effects of dual inhibitors, suggesting that SWI/SNF dependency in human leukemia extends beyond a predominantly BRG1-driven mechanism. Through gene expression and chromatin accessibility studies, we show that the dual inhibitors act at genomic loci associated with oncogenic transcription factors, and observe a downregulation of leukemic pathway genes, including MYC, a well-established target of BRG1 activity in AML. Overall, small-molecule inhibition of BRG1/BRM induced common transcriptional responses across leukemia models resulting in a spectrum of cellular phenotypes.

Implications:
Our studies reveal the breadth of SWI/SNF dependency in leukemia and support targeting SWI/SNF catalytic function as a potential therapeutic strategy in AML.
‘For the Sciences Migrate, Just Like People’: The Case of Botanical Knowledge in the Early Modern Iberian Empires
Authors: Segev Ran 
Published: November 2021

Abstract
In his writings, Francis Bacon emphasized the interrelatedness between the migration of people and knowledge, arguing that Europeans of his time had surpassed the greatest civilizations because of their ability to traverse the world freely. Concentrating on Spanish observers who investigated New Spain’s flora, this article bridges theory and practice by examining the Iberian roots of Bacon’s views. The article examines scientific approaches for acquiring bioknowledge by Iberians who specialized in European medicine, including Francisco Hernández, Juan de Cárdenas and Francisco Ximénez. While the article recognizes the contribution of travellers and expatriates to Spain’s bioprospecting project, it also points to the ways in which the limitations of the transfer of botanical information was acknowledged and discusses its meaning. By presenting the complexities in the communication of knowledge, I argue, naturalists in the colonies could highlight their unique vantage point in relation to “armchair” specialists in the metropole.
Controllable Summarization with Constrained Markov Decision Process
Authors: Chan Hou Pong , Wang Lu , King Irwin 
Published: November 2021

Abstract
We study controllable text summarization, which allows users to gain control on a particular attribute (e.g., length limit) of the generated summaries. In this work, we propose a novel training framework based on Constrained Markov Decision Process (CMDP), which conveniently includes a reward function along with a set of constraints, to facilitate better summarization control. The reward function encourages the generation to resemble the human-written reference, while the constraints are used to explicitly prevent the generated summaries from violating user-imposed requirements. Our framework can be applied to control important attributes of summarization, including length, covered entities, and abstractiveness, as we devise specific constraints for each of these aspects. Extensive experiments on popular benchmarks show that our CMDP framework helps generate informative summaries while complying with a given attribute’s requirement.1
The Attack on the US Capitol: An American Kristallnacht
Authors: Heine Jorge 
Published: November 2021

Abstract
The attack on the US Capitol on 6 January 2021 was the first such attack on the US Congress since the British-American War of 1812. It was also the event leading to the highest number of injured first responders from any single event in the US since 9/11. What led to it? To respond this question, this article places this event within the broader crisis of Western democracies and the rise of populism that has been its hallmark. It explains the attack on the Capitol as a result of the “Big Lie”, that is, the assertion that the November 3, 2020, presidential elction was stolen from Donald Trump. The remarkable resonance this unfounded claim has found among the US population, in turn, can be traced back to the huge division by race, class and geography currently affecting the United States, a division that makes for a highly polarized polity.
My Own Liberator: A South African Story
Authors: Moseneke Dikgang 
Published: November 2021

Abstract
Dikgang Moseneke was born in Pretoria, South Africa in December 1947. He was imprisoned on Robben Island, where most political prisoners were kept, off the coast of Cape Town for 10 years as a young man for his political activity. While in prison, he obtained a Bachelor of Arts degree in English and Political Science and later completed a Bachelor of Laws degree. After his release from prison, he was admitted as an attorney in 1978 and in 1983 was called to the Pretoria Bar as a Senior Counsel.
During the 1980s he worked underground for the Pan-African Congress and became its deputy president when it was unbanned in 1990. Moseneke also served on the technical committee that drafted the interim South African constitution of 1993. After a corporate career between 1995 and 2001, President Thabo Mbeki appointed him to the High Court in Pretoria and in 2002 as a judge in the Constitutional Court. In June 2005, he became the Court’s Deputy Chief Justice, a position from which he retired in May 2016.
In this essay, he chronicles his years of protest, political activity, and imprisonment as a young man. The essay is an excerpt from his memoir, My Own Liberator, which is published by Picador Africa (2018), and is available online and at all good bookstores.
Maternal Informal Learning Experiences that Shape Parenting Practices
Authors: Coolman Frances L. , Turnbull Khara L. P. , LoCasale-Crouch Jennifer , Moon Rachel Y. , Hauck Fern R. , Kellams Ann , Colson Eve 
Published: November 2021

Objective: Current understanding of the linkage between maternal education and parenting practices has largely been informed using a narrow definition of educational attainment—the highest level of education an individual has completed. However, the proximal processes that shape parenting, including informal learning experiences, are also important to understand. Less is known about the informal learning experiences that shape parenting decisions and practices. To this end, we conducted a qualitative inquiry about the informal learning experiences of mothers of children ages 3–4 years with the specific goal of understanding how maternal informal learning experiences shape parenting decisions and practices. Design: We conducted interviews with 53 mothers from across the United States who had previously participated in a randomized controlled trial (RCT) of an intervention targeting infant care practices. We recruited a purposive sample of mothers chosen to maximize diversity across educational attainment and adherence to infant care practices targeted in the RCT. Using a grounded theory approach, data were analyzed using an iterative process for organizing codes and themes that mothers identified as informal learning experiences. Results: We identified seven themes representing distinct types of maternal informal learning experiences that impact parenting practices, including: (1) experiential learning during childhood; (2) experiential learning during adulthood; (3) interpersonal interactions including via social media; (4) experiences with non-interactive media sources; (5) informal trainings; (6) beliefs; and (7) current circumstances. Conclusions: Multiple informal learning experiences inform the parenting decisions and practices of mothers with varying levels of formal educational attainment.
Social position and functional somatic disorders: The DanFunD study
Authors: Schovsbo Signe U. , Dantoft Thomas M. , Thuesen Betina H. , Leth-Møller Katja B. , Eplov Lene F. , Petersen Marie W. , Jørgensen Torben , Osler Merete 
Published: November 2021

Background and aim: It is generally accepted that functional somatic disorders (FSDs) are a product of biological, psychological, and social factors. Social position might be part of this complex, but the literature on this issue is currently heterogeneous and inconsistent. The aim of the present study was – in a population-based cohort – to test the hypothesis that lower social position would be associated with higher a risk of FSD. Method: The association between social position and FSD was examined in a cross-sectional study with various measures of social position (education as measured by vocational training; employment; cohabitation; subjective social status) and delimitations of FSD (irritable bowel syndrome, chronic fatigue syndrome, fibromyalgia, bodily distress syndrome, and symptom profiles). The associations were analyzed using logistic regressions to calculate odds ratios and 95% confidence intervals. Each social measure was analyzed independently and was adjusted for age and sex. Results: Lower levels of vocational training, being unemployed, and living alone were associated with higher risk of FSD, regardless of the FSD delimitation. There was also a significant negative association between subjective evaluated social status and FSD. The associations remained after multiple adjustments, and seemed to be strongest for the more severe FSD-types. Conclusions: Lower social position is associated with higher risk of FSD, especially the more severe FSD delimitations, which might constitute an especially vulnerable group. However, the mechanisms behind the relations remain unknown.
Clinical Significance of Myelin Oligodendrocyte Glycoprotein Autoantibodies in Patients with Typical MS Lesions on MRI
Authors: Zara Pietro , Floris Valentina , Flanagan Eoin P. , Lopez-Chiriboga A. Sebastian , Weinshenker Brian G. , Solla Paolo , Sechi Elia 
Published: November 2021

Background Myelin-oligodendrocyte-glycoprotein (MOG)-IgG-positivity in patients with typical MS lesions on MRI may lead to diagnostic/therapeutic uncertainty. Objective and Methods We reviewed reports of cases with MS phenotype on MRI and MOG-IgG-positivity published in Pubmed between 01/2012–06/2021. Results Sixteen patients were included (median age [range], 37,5 [25–66] years; 60% female). Three patients initially tested negative for MOG-IgG. Disease course was: relapsing-remitting, 10; or progressive, 6. Intrathecal IgG-synthesis was common (79%). Low and high-efficacy MS-targeted agents prevented relapses in 30% and 100%, respectively. None of the patients showed resolution of MRI T2-lesions over time. Conclusions MOG-IgG-positivity is unlikely to alter the expected treatment response and outcomes in patients with otherwise typical MS phenotype on MRI.
Occupational status and self-reported low back pain by gender: a nation-wide cross-sectional study among the general population in Japan
Authors: Tomioka Kimiko , Shima Midori , Saeki Keigo 
Published: November 2021

Abstract
Background
We aimed to examine the cross-sectional association between occupational class and self-reported low back pain (LBP) in a representative sample of the Japanese general population.

Methods
We used anonymized data from a nationwide survey (31,443 men and 35,870 women aged ≥ 20). Occupational class variables included working status, occupation, employment status, and company size (number of employees). Covariates included age, socio-economic status, lifestyle, and comorbidities. Poisson regression models stratified by gender were used to estimate adjusted prevalence ratio (APR) and 95% confidence interval (CI) for self-reported LBP.

Results
The prevalence of self-reported LBP was 11.7% in men and 14.2% in women. After adjustment for covariates and mutual adjustment for all occupational class variables, among both genders, agricultural/forestry/fishery workers and non-workers had a significantly higher prevalence of self-reported LBP: APR (95% CI) of agricultural/forestry/fishery was 1.36 (1.08–1.70) in men and 1.59 (1.30–1.93) in women; that of non-workers was 1.42 (1.18–1.70) in men and 1.23 (1.08–1.40) in women. Among men, non-regular employees were more likely to have self-reported LBP than regular employees: APR (95% CI) was 1.25 (1.07–1.46) in part-timers and casual staff and 1.18 (1.03–1.35) in other types of non-regular employees. Moreover, compared to men working at companies with ≥ 100 employees, men working at companies with 30–99 employees had a significantly higher prevalence of self-reported LBP (APR 1.17; 95% CI, 1.03–1.34). Among women, professionals and technicians (1.26; 1.11–1.43) and sales workers (1.22; 1.04–1.43) had a significantly higher prevalence of self-reported LBP than clerks. Neither employment status nor company size was associated with self-reported LBP in women. After stratified analyses by age group, similar patterns were observed in participants aged 20–64, but not in those aged ≥ 65.

Conclusion
Our results suggest that self-reported LBP is highly prevalent among agricultural/forestry/fishery workers and the unemployed, regardless of gender, and that there are also gender differences in the association of occupational class factors with self-reported LBP. It is necessary, therefore, to take preventive measures against LBP based on gender and occupational class factors in Japan.
A novel approach to co-expression network analysis identifies modules and genes relevant for moulting and development in the Atlantic salmon louse (Lepeophtheirus salmonis)
Authors: Zhou Zhaoran , Eichner Christiane , Nilsen Frank , Jonassen Inge , Dondrup Michael 
Published: November 2021

Abstract
Background
The salmon louse (Lepeophtheirus salmonis) is an obligate ectoparasitic copepod living on Atlantic salmon and other salmonids in the marine environment. Salmon lice cause a number of environmental problems and lead to large economical losses in aquaculture every year. In order to develop novel parasite control strategies, a better understanding of the mechanisms of moulting and development of the salmon louse at the transcriptional level is required.

Methods
Three weighted gene co-expression networks were constructed based on the pairwise correlations of salmon louse gene expression profiles at different life stages. Network-based approaches and gene annotation information were applied to identify genes that might be important for the moulting and development of the salmon louse. RNA interference was performed for validation. Regulatory impact factors were calculated for all the transcription factor genes by examining the changes in co-expression patterns between transcription factor genes and deferentially expressed genes in middle stages and moulting stages.

Results
Eight gene modules were predicted as important, and 10 genes from six of the eight modules have been found to show observable phenotypes in RNA interference experiments. We knocked down five hub genes from three modules and observed phenotypic consequences in all experiments. In the infection trial, no copepodids with a RAB1A-like gene knocked down were found on fish, while control samples developed to chalimus-1 larvae. Also, a FOXO-like transcription factor obtained highest scores in the regulatory impact factor calculation.

Conclusions
We propose a gene co-expression network-based approach to identify genes playing an important role in the moulting and development of salmon louse. The RNA interference experiments confirm the effectiveness of our approach and demonstrated the indispensable role of a RAB1A-like gene in the development of the salmon louse. We propose that our approach could be generalized to identify important genes associated with a phenotype of interest in other organisms.
Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
Authors: He Yilian , Chen Hua , Zhao Jun , Yang Yuxia , Yang Bin , Feng Liang , Zhang Yiguan , Wei Ping , Hou Dabin , Zhao Junning , Yu Ma 
Published: November 2021

Abstract
Background
Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic composition and the expressed genes involved in saikosaponin biosynthetic pathways among four organs (the root, flower, stem, and leaf) of B. chinense to discover the genes related to the saikosaponin biosynthetic pathway.

Results
Transcript and metabolite profiles were generated through high-throughput RNA-sequencing (RNA-seq) data analysis and liquid chromatography tandem mass spectrometry, respectively. Evaluation of saikosaponin contents and transcriptional changes showed 152 strong correlations (P < 0.05) over 3 compounds and 77 unigenes. These unigenes belonged to eight gene families: the acetoacetyl CoA transferase (AACT) (6), HMG-CoA synthase (HMGS) (2), HMG-CoA reductase (HMGR) (2), mevalonate diphosphate decarboxylase (MVD) (1), 1-deoxy-D-xylulose-5-phosphate synthase (DXS) (3), farnesyl diphosphate synthase (FPPS) (11), β-amyrin synthase (β-AS) (13) and cytochrome P450 enzymes (P450s) (39) families.

Conclusions
Our results investigated the diversity of the saikosaponin triterpene biosynthetic pathway in the roots, stems, leaves and flowers of B. chinese by integrated transcriptomic and metabolomic analysis, implying that manipulation of P450s genes such as Bc95697 and Bc35434 might improve saikosaponin biosynthesis. This is a good candidate for the genetic improvement of this important medicinal plant.
Glasgow Aneurysm Score: a predictor of long-term mortality following endovascular repair of abdominal aortic aneurysm?
Authors: Özen Anıl , Yılmaz Metin , Yiğit Görkem , Civelek İsa , Türkçü Mehmet Ali , Çetinkaya Ferit , Ünal Ertekin Utku , İşcan Hakkı Zafer 
Published: November 2021

Abstract
Background
To evaluate the value of Glasgow Aneurysm Score (GAS) in predicting long-term mortality and survival in patients who have undergone endovascular aortic aneurysm repair (EVAR) for abdominal aortic aneurysm (AAA).

Methods
A retrospective single-center study of 257 patients with non-ruptured AAA undergoing EVAR between January 2013 and 2021. GAS scores were compared between the survivors (group 1) and the long-term mortality (group 2) groups. Cox regression analysis was used to determine independent predictors of late mortality. Receiver operating characteristic curve (ROC) analysis was used to determine the optimum cut-off values of GAS values to determine the effect on late-mortality. Survival analysis was conducted using Kaplan-Meier.

Results
The study included 257 patients with a mean age of 69.75 ± 7.75 (46–92), who underwent EVAR due to AAA. Average follow up period was 18.98 ± 22.84 months (0–88). Fourty-five (17.8%) mortalities occured during long-term follow-up. A past medical history of cancer resulted in a 2.5 fold increase in risk of long-term mortality (OR: 2.52, 95% CI 1.10–5.76; p = 0.029). GAS values were higher in group 2 compared to group 1 (81.02 ± 10.33 vs. 73.73 ± 10.46; p < 0.001). The area under the ROC curve for GAS was 0.682 and the GAS cut-off value was 77.5 (specificity 64%, p < 0.001). The mortality rates in patients with GAS < 77.5 and GAS > 77.5 were: 12.8% and 24.8% respectively (p = 0.014). Every 10 point increase in GAS resulted in approximately a 2 fold increase in risk of long-term mortality (OR: 1.8, 95% CI 1.3–2.5; p < 0.001). Five year survival rates in patients with GAS < 77.5 and > 77.5 were 75.7% and 61.7%, respectively (p = 0.013).

Conclusions
The findings of our study suggests that an increase in GAS score may predict long-term mortality. In addition, the mortality rates in patients above the GAS cut-off value almost doubled compared to those below. Furthermore, the presence of a past history of cancer resulted in a 2.5 fold increase in long-term mortality risk. Addition of cancer to the GAS scoring system may be considered in future studies. Further studies are necessary to consolidate these findings.