bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Studies to correlation between gut microbiota diversity and functional erectile dysfunction
Authors: Geng Qiang , Chen Shaofeng , Sun Yuan , Zhao Yu , Li Zhong , Wang Fu , Yu Guojin , Yan Xiuchuan , Guo Jun 
Published: May 2021

Abstract
Objective: To analyze the distribution of gut microbiota in the ED patients, and explore the relationship between the diversity of gut microbiota and psychogenic erectile dysfunction. Methods: 30 cases of patients with erectile dysfunction (ED) and 30 healthy persons (healthy donor, HD) stool specimen were collected, using Illumina's Miseq platform samples V3-V4 region sequences bacterial 16SrRNA gene Paired end (PE) 300 sequencing, sequencing results were analyzed differences in species composition and diversity. Analysis contains five modules: sequencing data quality control, OTU species clustering and annotation, alpha diversity, beta diversity and the use of T-test and the analysis of the LEfSe differences. Results: 1. The flora diversity in the group of ED than HD significantly different (P<0.01), ED group has a low bacterial diversity. 2. Between ED group and HD group, abundant bacteria (TOPlO) and core flora (90%) had no significant difference in the genus level; all bacteria flora (>1%) display, Alloprevotella groups genus presents differences, Alloprevotella only be identified in the HD group. 3. ED and HD groups present in well separated PCoA analysis, having a significant difference in the two kinds of microflora. 4.T-test shows six species were significantly different, in the ED group, Streptococcus and Subdoligranulum were increasing, and Prevotella, Prevotella sp.9, Blautia, Lachnospiraceae NK4A136 groups and Roseburia were decreasing. 5.LEfSe analysis revealed 24 species were significantly different between ED and HD groups. Conclusion: Gene sequencing was performed on the two groups of specimens and finding that microbial community structure and diversity had significant difference, suggesting that ED have low gut microbiota diversity.
Effect of Neuromuscular electrical stimulation on postoperative complications after artificial abortion ---A retrospective study
Authors: SHU JUNJUN , Lin Shixin , Wu Yu , Wang Xiaojiao , Zhu Hong , Gao Jun 
Published: May 2021

Abstract
Background: The complications after induced abortion are the important factors that endanger female reproductive health. The effective prevention way of postoperative complications after induced abortion and protection of female reproductive tract health is now a hot issue. This retrospective study aimed to evaluate the effect of neuromuscular electrical stimulation (NMES) on postoperative complications after artificial abortion.Methods: A total of 148 patients were randomized into 2 groups in this study. 76 patients were assigned into a treatment group which also called group A, and underwent NMES therapy, while 72 women were assigned into a controlled team named group B. The duration of postoperative uterine contraction pain, the amount and duration of postoperative vaginal bleeding, the endometrial thickness on the 14th and 21st day after the operation, the time of postoperative menstruation recovery, and the incidence of intrauterine adhesion on the 1st and 3rd month after operation were compared and observed.Results: Compared with group B, in group A, The duration of postoperative uterine contraction pain was significantly shorter(P<0.001); The amount and duration of postoperative vaginal bleeding were less after treatment by the NMES (P=.016); There was no significant difference in endometrial thickness on the 14th day after the operation(P=.05), Whereas it was thicker in the observation group than in the control group on the 21st day(P=.01); The menstruation recovery time was shorter after treatment (P=.017); The incidence of intrauterine adhesions was significantly lower at the third months after operation (P=.03)while at the first month, there is no difference(p>0.99).Conclusion: Neuromuscular Electrical stimulation therapy is effective for improving patients with postoperative complications after artificial abortion.
The relative risk of the toxico-clinical parameters based on poisoning severity and outcomes in patients with acute poisoning
Authors: Eizadi-Mood Nastaran , Heshmat Rasol , Meamar Rokhsareh , Motamedi Narges 
Published: May 2021

Abstract
Background: Identification of complications or death risk factors in patients with acute poisoning allows better monitoring and treatment. Hence, the aim of this study was to determine the relative risk of toxico-clinical parameters based on poisoning severity and outcomes in patients with acute poisoning. Methods: This cross-sectional study consisted of patients with acute poisoning admitted to the poisoning emergency room from 2018 to 2019. Patients were categorized into four groups minor, moderate, severe, and fatal poisoning. Different toxico-clinical parameters were analyzed by multivariate logistic regression. Results: The most common poisoning in the minor group was opioids, alcohols, and benzodiazepine (14.7%), in the moderate and severe groups was multidrug (23.3%) and in the fatal group pesticides poisoning (23%). Pre-hospital antidote administration (OR, 7.08; P-value, 0.006); low level of consciousness (OR, 4.38; P-value, 0.001); abnormal ECG (OR, 4.56; P-value, 0.003); and time interval of intoxication to admission in the hospital (OR, 1.15; P-value, 0.01) were the predictive factors for poisoning severity. 49.7% of patients recovered without any complications. Patients with low level of consciousness (OR, 66.06; P-value, 0.01); underlying disease (OR, 3.65; P-value, 0.03); abnormal respiration (OR, 1.14; P-value, 0.02); and longer duration of hospitalization (OR, 1.02; P-value, 0.001) had greater risk of complications/ Death. Conclusions: In clinical practice, low level of consciousness, pre-hospital antidote administration, abnormal ECG, underlying disease, abnormal respiration, delay to presentation to hospital, and longer length of hospital stay can be considered important factors for determining poisoning severity and outcome.
Optimizing Reduction of Groundwater Discharge to Control Land Subsidence (Case study: Nadjafabad Aquifer)
Authors: moghaddam hamid Kardan , kivi Zahra Rahimzadeh , Javadi Fatemeh , Heydari Mohammad 
Published: May 2021

Abstract
This study evaluates and predicts the ground subsidence that happens due to the haphazard operation of groundwater resources. Also, several strategies have been developed to control this unpleasant phenomenon. For this purpose, groundwater flow simulation has been conducted using MODFLOW numerical model, and subsidence simulation in Najafabad plain has been done using SUB package under three climatic scenarios for future periods. Examination of the simulation results shows that the amount of land subsidence will increase with the aquifer operation's continuation. The maximum amount of subsidence for 6 years in drought conditions will be 23 cm at the aquifer's outlet. According to the land subsidence results at the aquifer, risk zoning of the aquifer operation was done to develop a solution to reduce the withdrawal of groundwater resources to control subsidence. Therefore, risk zoning was performed using land use and the extent of operation of groundwater resources. The results showed that the north-eastern part of the aquifer has the maximum risk of subsidence. According to the obtained results from subsidence risk zoning, scenarios of reduced water withdrawal from the aquifer in its outlet were developed. The treatment strategies results showed that the maximum amount of subsidence in wet, normal and dry conditions will be 10, 14 and 18 cm, respectively. These results indicate a 14% improvement in the quantitative condition of the aquifer in wet conditions, 10% in normal conditions and 7% in dry conditions in the total aquifer of Najafabad. Improvement of conditions by simulation shows the impact of the importance of optimal utilization of groundwater resources.
High-dose Radiotherapy in Newly Diagnosed Low-grade Gliomas with Nonmethylated O6-methylguanine-DNA Methyltransferase
Authors: Liu Yanwei , Li Yanong , Wang Peng , Chen Li , Feng Jin , Qiu Xiaoguang 
Published: May 2021

Abstract
Background: Patients with low-grade gliomas (LGGs) harboring O6-methylguanine-DNA methyltransferase promoter nonmethyaltion (MGMT-non-pM) have a particularly short survival and are greatly resistance to chemotherapy. The objective of this study was to assess the efficacy of high-dose radiotherapy (RT) for LGGs with MGMT-non-pM.Methods: 269 patients with newly diagnosed adult supratentorial LGGs from the multicenter Chinese Glioma Cooperative Group (CGCG) received postoperative RT during 2005-2018. MGMT promoter methylation analysis was conducted by pyrosequencing in all patients. Univariate and multivariable analyses were performed using the cox regression to determine the prognostic factors for overall survival (OS) and progression-free survival (PFS). RT dose-response on MGMT status defined subtypes was analyzed.Results: On univariate analysis, the following were statistically significant favorable factors for both PFS and OS: oligodendrogliomas（p = 0.002 and p = 0.005）, high-dose RT (> 54 Gy) （p = 0.017 and p = 0.023） and 1p/19q codeletion （p <0.001 and p = 0.001）. On multivariable analyses, RT dose (> 54 Gy vs. ≤ 54 Gy) and IDH mutation were independently prognostic markers for OS (HR, 0.44; 95%CI, 0.21-0.92; p = 0.029; and HR, 0.43; 95%CI, 0.20-0.90; p = 0.025, respectively) and PFS (HR, 0.48; 95%CI, 0.27-0.90; p = 0.021; and HR, 0.52; 95%CI, 0.27-0.98; p = 0.044, respectively). High-dose RT was associated with longer OS (HR, 0.55; 95%CI, 0.32-0.93; p = 0.026) and PFS (HR, 0.57; 95%CI, 0.35-0.93; p = 0.026) than low-dose RT in MGMT-non-pM subtype. In contrast, no significant difference in either OS (p = 0.240) or PFS (p = 0.395) was observed with high-dose RT in the MGMT-pM subtype.Conclusions: High-dose RT (> 54 Gy) is an independently protective factor for LGGs and associated with improved survival in patients with MGMT-non-pM.
Nested Case-Control Studies With Multiple Measurements: Estimating Marginal Relationships
Authors: Sampson Joshua N. , Albert Paul S. , Purdue Mark P. 
Published: May 2021

Abstract
Background: We consider the analysis of nested, matched, case-control studies that have multiple biomarker measurements per individual. We propose a simple approach for estimating the marginal relationship between a biomarker measured at a single time point and the risk of an event. We know of no other standard software package that can perform such analyses while explicitly accounting for the matching. Results: We propose an application of conditional logistic regression (CLR) that can include all measurements and uses a robust variance estimator. We compare our approach to other methods such as performing CLR with only the first measurement, CLR with an average of all measurements, and Generalized Estimating Equations. In simulations, our approach is significantly more powerful than CLR with one measurement or an average of all measurements, and has similar to power to GEE but correctly accounts for the matching. We then apply our approach to the CLUE cohort to show that an increased level of the immune marker sCD27 is associated with non‐Hodgkin lymphoma (NHL) and, by evaluating the strength of the association as a function of time until diagnosis, that the an increased level is likely an effect of the disease as opposed to a cause of the disease. The approach can be implemented by the R function clogitRV available at https://github.com/sampsonj74/clogitRV.Conclusion: We offered an approach and software for analyzing matched case-control studies with multiple measurements. We demonstrated that these methods are accurate, precise, and statistically powerful.
The Construction and Analysis of ceRNA Network and Immune Infiltration in Kidney Renal Clear Cell Carcinoma
Authors: Deng Lugang , Qu Zhi , Wang Peixi , Liu Nan 
Published: May 2021

Abstract
Purpose

Kidney renal clear cell carcinoma (KIRC) has the highest invasion, mortality and metastasis of the renal cell carcinomas and seriously affects patients’ quality of life. However, the composition of the immune microenvironment and regulatory mechanisms at transcriptomic level such as ceRNA of KIRC are still unclear.
Methods

We constructed a ceRNA network associated with KIRC by analyzing the long noncoding RNA (lncRNA), miRNA and mRNA expression data of 506 tumor tissue samples and 71 normal adjacent tissue samples downloaded from the Cancer Genome Atlas (TCGA) database. In addition, we estimated the proportion of 22 immune cell types in these samples through “CIBERSORT”. Based on the ceRNA network and immune cells screened by univariate Cox analysis and Lasso regression, two nomograms were constructed to predict the prognosis of patients with KIRC. Receiver operating characteristic curves (ROC) and calibration curves were employed to assess the discrimination and accuracy of the nomograms. Consequently, co-expression analysis was carried out to explore the relationship between each prognostic gene in a Cox proportional hazards regression model of ceRNA and each survival-related immune cell in a Cox proportional hazards regression model of immune cell types to reveal the potential regulatory mechanism.
Results

We established a ceRNA network consisting of 12 lncRNAs, 25 miRNAs and 136 mRNAs. Two nomograms containing seven prognostic genes and two immune cells, respectively, were successfully constructed. Both ROC [Area Under Curves (AUCs) of 1, 3 and 5-year survival in the nomogram based on ceRNA network: 0.779, 0.747 and 0.772; AUCs of 1, 3 and 5-year survivals in nomogram based on immune cells: 0.603, 0.642 and 0.607] and calibration curves indicated good accuracy and clinical application value of both models. Through co-correlation analysis between ceRNA and immune cells, we found both LINC00894 and KIAA1324 were positively correlated with follicular helper T (Tfh) cells and negatively correlated with resting mast cells.
Conclusions

Based on the ceRNA network and tumor-infiltrating immune cells, we constructed two nomograms to predict the survival of KIRC patients and demonstrated their value in improving the personalized management of KIRC.
Comparative Study On Load Resistance of Eco-Friendly Interlocking Blocks For Sustainable Construction
Authors: Chunduri Venkata Veera Himakar , Khed Veerendrakumar C. 
Published: May 2021

Abstract
The rapid growth of contemporary construction industry has driven engineers to explore new construction techniques for sustainable development. Interlocking block wall construction reduces time, labour and enhances construction efficiency compared to conventional masonry wall construction. The interlocking pattern has been applied to the top and bottom surface of block to withstand gravity and lateral loads whereas current interlocking blocks only serve the purpose of easy alignment. In this study, eco-friendly blocks have been developed with industrial waste such as fly ash, quarry dust and geopolymer as binder. Tests to evaluate the compressive strength, water absorption and efflorescence have been carried out on both traditional and geopolymer interlocking blocks. Later, on two blocks joint, in-plane vertical load has been applied and the same model is generated to validate the failure. Using numerical modelling, horizontal and vertical load resistance of interlocking block wall and traditional brick wall was compared. The experimental results show that, relative to traditional clay brick, the compressive strength of the geopolymer interlocking block was high but the absorption of water was low. The vertical load resistance is identical but horizontal load resistance was high in interlocking block wall. The interlocking geopolymer block was the best approach for environmental sustainability.
Administration of hydro-alcoholic extract of spinach improves oxidative stress and inflammation in high-fat diet-induced NAFLD rats
Authors: Amirinejad Ali , Totmaj Ali Saneei , Mardali Farzaneh , Hekmatdoost Azita , Emamat Hadi , Safa Majid , Shidfar Farzad 
Published: May 2021

Abstract
Background Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. The aim of this study was to evaluate the effects of hydro-alcoholic extract of spinach (HES) on hepatic and serum measurements of NAFLD in a rat model. Methods During the prevention phase, 18 Sprague–Dawley rats were fed one of the following regimens: a high-fat diet, a high-fat diet plus 400mg/kg HES, or a chow diet ad libitum for seven weeks. Before the treatment phase, NAFLD was induced in 24 rats. Afterwards, they were fed one of the following regimens: the same NAFLD-inducing diet, high-fat diet plus 400mg/kg HES, the chow diet, or chow diet plus 400mg/kg HES ad libitum for four weeks (n=6 in each group). Results Administration of HES combined with HF diet in rats was associated with decreased food intake (P< 0.01), weight loss (P=0.01), and increased SOD (P= 0.02) enzyme activity in the liver at the end of the prevention phase. hs-CRP (P< 0.05), PTX-3 (P< 0.05), PPAR-γ, and TNF-α gene expression in the liver were improved by spinach intake (P< 0.05), both in the prevention and treatment phases. Furthermore, administration of spinach in the treatment phase increased serum TAC (P=0.03) and hepatic GPX (P=0.01) enzyme activity. Conclusion Taking into account beneficial effects of HES on prevention and treatment of NAFLD observed in the present study, we propose that further human-based clinical investigations be conducted on subjects with NAFLD.
Old Does Not Necessarily Mean Worse: Standard 6-Week Chemoradiation For Elderly Patients (≥ 70 Years) With Newly Diagnosed Glioblastoma
Authors: Vaugier Loig , Ah-Thiane Loïc , Aumont Maud , Jouglar Emmanuel , Campone Mario , Colliard Camille , Doucet Ludovic , Frenel Jean-Sébastien , Gourmelon Carole , Robert Marie , Martin Stéphane-André , Riem Tanguy , Roualdes Vincent , Campion Loïc , Mervoyer Augustin 
Published: May 2021

Abstract
IntroductionGlioblastoma (GBM) is frequent in elderly patients, but their frailty provokes debate regarding optimal treatment in general, and the standard 6-week chemoradiation in particular, although this is the mainstay for younger patients. MethodsAll patients with newly diagnosed GBM and age ≥ 70 who were referred to our institution for chemoradiation (RCT) were reviewed from 2004 to 2018. MGMT status was not available for treatment decision at that time. The primary endpoint was overall survival (OS). Secondary outcomes were progression-free survival (PFS), early adverse neurological events without neurological progression ≤ 1 month after RCT and temozolomide hematologic toxicity assessed by CTCAE v5. Results128 patients were included. The median age was 74.1 (IQR: 72-77). 15% of patients were ≥80 years. 62.5% and 37.5% of patients fulfilled the criteria for RPA class I-II and III-IV, respectively. 81% of patients received the entire RCT and 28% completed the maintenance temozolomide. With median follow-up of 11.7 months (IQR: 6.5-17.5), median OS was 11.7 months (CI95%: 10-13 months). Median PFS was 9.5 months (CI95%: 9-10.5 months). 8% of patients experienced grade ≥3 hematologic events. 52.5% of patients without neurological progression had early adverse neurological events. Post-operative neurological disabilities and age ≥80 were not associated with worsened outcomes. Conclusions6-week chemoradiation was feasible for “real-life” elderly patients diagnosed with glioblastoma, even in the case of post-operative neurological disabilities.
HIV-1 Suppresses Notch-1 Expression in HPV-16+ CaSki Cells for Cancer Progression
Authors: DSouza Serena Judith , Mane Arati , Patil Linata , Shaikh Aazam , Thakar Madhuri , Saxena Vandana , Abadi Leila Fotooh , Godbole Sheela , Kulkarni Smita , Gangakhedkar Raman , Shastry Padma , Panda Samiran 
Published: May 2021

Abstract
Background

High Risk Human Papilloma Viruses (HR-HPV) recurrently infect women having Human Immunodeficiency Virus − 1 (HIV-1)infection. Transforming HPV E6 and E7 genes promote invasive cancers and interact with Notch-1receptor. Concomitantly, HIV-1 Tat binds to EGF motifs within the Notch-1extracellular domain. HR-HPV infection activates Notch-1 signalling. Permissive HIV-1entry into the cervix is allowed. Notch-1 inhibitors may offer solace to the aggressive cancer phenotype in HIV-1 positive women. Still, the molecular cross talk between different oncogenes within the Notch-1pathway during HIV-1/HPV-16 + co-infections has not been elucidated.
Methods

Adherent cervical tumor derived cell lines-CaSki cell line (with inherent HPV16+ sequence and endogenous Notch-1 activity) and C33A cell line (cervical cancer phenotype, HPV -ve for HPV DNA and RNA) were used. Plasmids (pLEGFPN1 encoding HIV-1 Tat and pNL 4 − 3 encoding HIV-1(full HIV-1 genome), were transfected at 600 ng/mL into the above mentioned cell lines, in order to examine independent effects of HIV-1 Tat and HIV-1 transfection in HPV-16+ cervical cancer cells. We performed western blotting, cell cycle analysis and RT-qPCR post transfection. Three sets of independent experiments were analyzed by Graph Pad Prism 5. Statistical significance was calculated using Student t -test. Data expressed as mean ± standard deviation (SD). p values ≤ 0.05* were considered statistically significant.
Results

HIV-1 Tat and HIV-1 inhibited Notch-1expression, with differential effects on EGFR when comparing C33A and CaSki cell lines. CDK2 induction in Tat transfected CaSki cells, showed concomitant G0/G1 phase accumulation favouring cancer progression. Notch-1 inhibition shut off significant Cyclin D expression with a significant p21 induction and increased G2-M cell population in CaSki cells. On the contrary, HIV-1 infection utilized Hes-1-EGFR-CyclinD-p21axis, G2-M arrest, DDR response and cancer progression.
Conclusion

Our study highlights for the first time that HIV-1 Tat and/or HIV-1 driven cancers in HPV-16+ CaSki cells, show Notch-1 suppression and CDK2 dependent activity. HIV-1 Tat activates Hes-1 amplifying the EGFR gene which improves the aggressive state possibly through irreversible oxidative-stress induced senescence. HIV-1, favours cancer progression through its CXCR4 receptor, responsible for unbridled mitosis, G2-M arrest and damaged DNA response (DDR). Treatment of CaSki cells with a Notch-1 inhibitor, DAPT showed marginal recovery in p21expression with Go/G1 and S phase recovery.