bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERAN PEMERINTAH DAERAH DALAM MEMBANGUN CITY BRANDING DI KOTA TANJUNGPINANG (STUDI KASUS KAMPUNG PELANGI)
Authors: Hartono Erfan , Safitri Dian Prima , Kurnianingsih Fitri 
Published: April 2021

<p>Penelitian ini berdasarkan dari sebuah fenomena dimana pada tahun 2018 dibangun sebuah Kampung Pelangi di Kota Tanjungpinang. Kampung Pelangi yang merupakan pilot projek pembangunan Kampung Wisata Baru di Kota Tanjungpinang, menjadi salah satu terobosan baru untuk mengubah image kampung kumuh menjadi kampung yang indah dan dikenal melalui <em>City branding </em>Kota Tanjungpinang yang bertajuk “Pesona Tanjungpinang”. <em>City branding</em> merupakan strategi dari sebuah kota atau daerah untuk membuat <em>positioning</em> yang kuat sehingga kota dan daerah tersebut dikenal secara luas. Penelitian ini menggunakan pendekatan kualitatif deskriptif untuk mengetahui Peran Pemerintah Daerah dalam membangun <em>City Branding</em> di Kota Tanjungpinang terkait <em>positioning</em> kampung wisata baru yaitu Kampung Pelangi yang dianggap menjadi ikon terbaru di Kota Tanjungpinang. Penelitian ini menggunakan dua subjek yaitu Dinas Pariwisata dan Kebudayaan Kota Tanjungpinang dan Kelurahan Melayu Kota Piring yang terlibat dalam Penanganan Kampung Pelangi. Teknik pengumpulan data dilakukan dengan observasi, wawancara serta dokumentasi. Hasil penelitian menunjukan bahwa Peran Pemerintah Daerah yang dilakukan oleh Dinas Pariwisata dan Kebudayaan Kota Tanjungpinang dalam membangun <em>City Branding </em>di Kota Tanjungpinang melalui Kampung Pelangi,<em> </em>yaitu: 1) Survei Pemetaan<em> (Mapping Survey)</em>, 2) Analisis Kompetitif<em> (Competitive Analysis), </em>3)<em> </em>Cetak Biru (<em>Blueprint</em>), dan 4) Implementasi<em> (Implementation) </em>masih belum optimal. Pemerintah daerah perlu mendorong pencapaian tujuan pembangunan kepariwisataan dan harus bisa menyediakan infrastruktur dan kerangka regulasi yang dapat mendorong swasta dan masyarakat untuk ikut berpartisipasi aktif dalam pembangunan kepariwisataan berbasis kampung wisata.</p>
SINERGITAS PEMERINTAH DESA DAN KELEMBAGAAN LOKAL SUBAK DALAM MEWUJUDKAN PEMBANGUNAN BERKELANJUTAN BERBASIS SUBAK SEBAGAI WARISAN BUDAYA DUNIA ( STUDI KASUS : SUBAK JATILUWIH, KABUPATEN TABANAN)
Authors: Febriantini Komang Trisna , Indriani Ni Kadek , Kusuma Bima Oktadinata , Yuniari Ni Komang Yuli 
Published: April 2021

Penelitian ini bertujuan untuk mengetahui sinergitas pemerintah desa dengan kelembagaan lokal subak dalam mewujudkan pembangunan berkelanjutan di daerah pedesaan berbasis Subak sebagai warisan budaya dunia (WBD) dimana WBD merupakan terobosan wisata alternatif yang akan dapat mensinergikan potensi Desa Jatiluwih berdasarkan budaya, mempertahankan keberadaan potensi tempat wisata dan meningkatkan ekonomi masyarakat sekitar berdasarkan peran sinergi dari pemerintah desa dan lembaga subak yang ada. Penelitian ini ingin mengetahui sinergi pemerintah desa dan lembaga subak lokal dalam mewujudkan pembangunan berkelanjutan ditinjau dari segi komunikasi dan koordinasi menurut Najiyati (2011) dan dianalisis menggunakan metode deskriptif kualitatif yang menghasilkan data deskriptif yang diperoleh melalui penelitian kepustakaan, observasi, dokumentasi, dan wawancara mendalam dengan informan. Hasil dari penelitian ini diharapkan dapat menjelaskan proses koordinasi dan komunikasi antara Pemerintah Desa Jatiluwih dan kelembagaan lokal subak dalam pembangunan desa termasuk kendala yang dialami dalam proses komunikasi dan koordinasi.
Cyclone Oil Desorption Technology for the Disposal of Oil-Based Mud Cuttings
Authors: Wu Jiwei , Pan Jiake , Wang Hualin , Wang Lixiang , Liu Wenjin , Zhang Le 
Published: April 2021

Summary
With the flourishing shale gas exploitation producing more oil-based mud (OBM) cuttings, the hard-to-treat hazardous wastes heavily burden the local environment. However, the problems of treating OBM cuttings, such as huge energy consumption, tremendous treatment costs, and high risk of secondary contamination, still remain unsolved with the current treatment technologies, such as thermal desorption, incineration, and chemical extraction.
In this study, we introduce a new method and equipment based on cyclone desorption to recover oil from OBM cuttings. The technological process includes viscosity reduction in heated gas, cyclone deoiling, condensation and recycling of the exhaust, and separation of oil and water in the coalescer. Based on the analysis of the physicochemical properties and the oil distribution inside the OBM cuttings samples collected from the Chongqing shale gas field, we designed this cyclone oil desorption technology and built the pilot-scale equipment to conduct the deoiling experiments. The results showed that the deoiling efficiency of OBM cuttings improved as the processing time increased. To be precise, after 2.7 seconds of treatment, the oil content of the cuttings samples fell sharply from 17.9 to 0.16%, which is about one-half of the maximum allowable oil content in pollutants of 0.3%, specified in the national standard (GB 4284-84 1985) promulgated by the People’s Republic of China.
The foundation of the technology is that the particles have a high-speed self-rotation (more than 30,000 rad/s) coupled with a revolution in the cyclone in which a generated centrifugal force removes the oil from the pores of the particles. This process is purely physical and involves no phase change of the oil, so it is free of chemical addition and high heating temperature. The application of this newly developed cyclone oil desorption technology is expected to lower the treatment costs, enhance the processing efficiency, contribute to the energy development, and eventually benefit the local environment where the shale gas exploitations take place.
Prenatal polychlorinated biphenyl exposure promotes invasion of progeny ectopic endometrial stromal cells via epigenetic modification of EZH2
Authors: Wang Yinfeng , Ye Chaoshuang , Hu Changchang , Chen Pei , Chen Chaolu , Wu Ruijin 
Published: April 2021

Abstract
Polychlorinated biphenyls(PCBs) are persistent environmental endocrine disruptor. This study aim to investigate the changes of the ectopic endometrium invasive ability and the possible mechanism after exposure to PCBs during pregnancy. In total, 12 female Sprague Dawley rats were intraperitoneally injected with Aroclor 1221 (1 mg/kg) or dimethyl sulfoxide (1 mg/kg) at 16 and 18 days of gestation. The endometriosis model was established by autogenous uterine abdominal wall implantation 2 months after birth. The degree of adhesion between the endometriosis and the greater omentum, as well as cell morphology and Transwell invasion patterns were used to evaluate the invasive ability of progeny ectopic endometrial stromal cells (ESCs). Moreover, the effect of the enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2)/ trimethylation of Histone 3 lysine 27 (H3K27me3) axis was examined using a highly selective EZH2 inhibitor, GSK126. After gestational PCBs exposure, the adhesion between the endometrium and the greater omentum was enhanced (P<0.05), and the length and number of microvilli were significantly increased (P<0.01). Exposure to PCBs during pregnancy increased the invasive ability of the ectopic ESCs (P<0.01), with upregulated expression levels of EZH2 and H3k27me3, which were abrogated by the EZH2 inhibitor GSK126. In conclusion, exposure to PCBs during pregnancy increased the invasive ability of the ectopic endometrium, which may be mediated by EZH2.
Study on Photodegradation&nbsp;Activity of Fe3O4@SiO2@TiO2-Ce/rGO Magnetic Photocatalyst
Authors: Cao Weiyan , Xijun Liu , Yuwei Wang , Yong Zhang , Xianxin Han , Congzhi Fu 
Published: April 2021

Abstract
The magnetic core-shell Fe3O4@SiO2@TiO2-Ce/rGO composite nanomaterials were prepared by sol-gel method and hydrothermal method, composite photocatalyst doped with metal and loaded with graphene, the catalytic activity has been greatly improved. Under normal experimental conditions (pH = 7, [MB] = 10mg/L, magnetic composite photocatalyst concentration = 0.1g/50mL), Fe3O4@SiO2@TiO2-Ce/rGO nano-composites photocatalyst maximum degrade MB reaches 98.2% in 140 minutes.
Determination of the Youngest Active Domain in Major Fault Zones Using Medical X-ray Computed Tomography-derived Density Estimations
Authors: iwamori akiyuki , Takagi Hideo , Asahi Nobutaka , Sugimori Tatsuji , Nakata Eiji , Nohara Shintaro , Ueta Keiichi 
Published: April 2021

Abstract
Determination of the youngest active domains in fault zones that are not overlain by Quaternary sedimentary cover are critical for evaluating recent fault activity, determining the current local stress field, and mitigating the impacts of future earthquakes. Considering the exhumation of a fault zone, the youngest active domain in a fault zone is supposed to correspond to the activity at the minimum fault depth of a buried fault, such that the most vulnerable area, which possesses the lowest rock/protolith density ratio, is assumed to be indicative of this recent fault activity. However, it is difficult to measure the density of fault rocks and map the rock/protolith density ratio across a given fault zone. Here we utilize medical X-ray computed tomography (CT), a non-destructive technique for observing and analyzing materials, to investigate the fault characteristics of several fault zones and their surrounding regions in Japan, and attempt to determine the youngest active domain of a given fault zone based on its CT numbers, which are a function of the density and effective atomic number of the fault rock and protolith. We first investigate the density, void ratio, and effective atomic number of active and inactive fault rocks, and their respective protoliths. We then calculate the CT numbers after reducing the beam-hardening effects on the rock samples, and study the relationships among the CT number, density, and effective atomic number. We demonstrate that the density, effective atomic number, and CT number of the fault rock decrease as the youngest active zone is approached, such that the region with the lowest CT number and rock/protolith density ratio defines the youngest active domain of a given fault zone.
Expression of Recombinant Protein of Coat Protein Gene of Sweet Potato Feathery Mottle Virus in Bacterial Expression System and Production of Polyclonal Antiserum for Its Diagnosis
Authors: Thangaraj Makeshkumar , T Jayakrishnan J 
Published: April 2021

Abstract
Sweet potato (Ipomoea batatus L. (Lam), Family Convolvulaceae) is one of the most important tuber crops providing nutritional security because of its high consumption value and medicinal properties, and numerous agro-industrial uses. Sweet potato feathery mottle disease caused by Sweet potato feathery mottle virus (SPFMV) is one of the serious constrains in sweet potato cultivation in India. Effective diagnostic methods need to be developed to solve the problem due to these viruses. As part of the study, infected leaf samples from fields were collected, positive samples were screened for SPFMV using DAC-ELISA and confirmed through PCR. Coat protein gene of SPFMV was PCR amplified, cloned into TA cloning vector and then transformed into Escherichia coli DH5α cells. Positive clones were sub cloned into expression vector pET28A(+) and transformed into DH5α cells. Plasmid DNA from positive clones were isolated and transformed into BL21DE3 cells (NiCo21-DE3 cells). Positive clones were identified and confirmed in-frame position through sequence analysis. Selected colony was grown in Luria both medium at 37oC. Cells were collected and solubility of SPFMV coat protein (CP) was checked through SDS PAGE. Various standardisations were carried out for optimising expression of SPFMV CP and it was observed that 4 hr induction of 1.5 mM IPTG at 25oC gives maximum yield. Using these conditions, cells were grown on large scale and purified the protein (SPFMV CP) using Ni-NTA resin affinity chromatography. Purified protein was checked using SDS PAGE, confirmed the expression using Western Blotting and given for immunization into two New Zealand white rabbits for polyclonal antibody production. Serological tests like ELISA and DIBA were done for confirming the sensitivity and specificity of the raised antibody using field samples of SPFMV infected sweet potato along with healthy plants. Tested samples gave strong positive reactions at dilutions of 1:500 up to 1:6000. Also antibody reacted specifically at a dilution of 1:6000 in ELISA and DIBA. This is the first report of development of polyclonal antiserum against CP of SPFMV through recombinant technology in India and can be useful for the detection of virus from the field-grown samples.
Application of a Targeted and Quantificational Foraminoplasty Device in Percutaneous Transforaminal Endoscopic Discectomy for L5-S1 Disc Herniation: Preliminary Clinical Outcomes
Authors: Liu Jinlong , wu Junlong , Zhang Honglei , Zuo Rui , Liu Jiabin , Zhang Chao 
Published: April 2021

Abstract
ObjectivePercutaneous transforaminal endoscopic discectomy (PTED) is minimally invasive and has been widely used to treat patients with lumbar disk herniation(LDH) due to its safety and efficiency. However, due to the unique anatomy of the L5-S1 level, the PTED procedure is often difficult to perform in the region. ZESSYS, a targeted and quantificational foraminoplasty device, may help to overcome these anatomical limitations. In this study, we assessed the efficiency and the short-term eﬀects of PTED with ZESSY at the L5-S1 level.MethodsBetween January to August of 2018, Fifty-six patients with lumbar disk herniation at the single level of L5–S1 and underwent percutaneous transforaminal endoscopic discectomy were enrolled in this retrospective cohort study. They were segregated into the the TESSYS group and the ZESSYS group. The puncture time, foraminoplasty time, decompression time and fluoroscopy time evaluated for the operation efficiency. Clinical outcomes were assessed by visual analog scale(VAS) score and Oswestry Disability Index(ODI) score. The MacNab criteria were used to evaluate patient subjective satisfaction at 12-month follow-up postoperatively.ResultsThe average puncture time (5.29±2.05 minutes), foraminoplasty time (12.82±2.52 minutes) and fluoroscopy time (26.29±5.96 seconds) were all significantly shorter in the ZESSYS group than in the TESSYS group (average puncture time 8.07±3.13 minutes, p<0.01; foraminoplasty time, 17.18±2.92 minutes, p<0.01; fluoroscopy time, 34.73±6.86 seconds; p<0.01). No significant differences were observed between the 2 groups in the decompression time (p=0.057). The VAS score of low back pain and leg pain, as well as the ODI score, improved at all time points postoperatively compared with preoprative , in both the TESSYS group and the ZESSYS group (P < 0.05). There were no significant differences in the VAS score of low back pain, VAS score of leg pain, and ODI score between the TESSYS group and the ZESSYS group at the same time points (P > 0.05). According to the MacNab criteria, the excellent and good rate at 12-month follow-up postoperatively was 85.7% in the TESSYS group and 89.3% in the ZESSYS group (P > 0.05).ConclusionThe targeted and quantificational foraminoplasty device named ZESSYS was more efficient in the puncture and foraminoplasty procedures, effectively protecting the exiting nerve and minimizing the level of radiation exposure. The device is efficient and safe for PTED in treating lumbar disk herniation at the L5-S1 level.
Research on the Mechanism of Wuwei Yuganzi San in the Treatment of Coronary Heart Disease Based on Network Pharmacology
Authors: Zhang Qunhui , Li Yimei , Guo Yang , Lu Qiqin , Zhang Guoying , Ma Jing , Zhang Dejun 
Published: April 2021

Abstract
Background: Coronary heart disease (CHD) is a chronic cardiovascular disease across the world, which poses numerous threats to mankind. Wuwei Yuganzi San (WYS) is a famous traditional Tibetan medicine prescription. It has been confirmed effective in the treatment of CHD, but its specific mechanism remains still unclear. Objective: To elucidate the main pharmacological action of WYS in treating CHD and investigate the underlying multiple mechanisms of its multi-ingredient-multi-target by network pharmacology.Methods: Firstly, active ingredients of WYS and connected targets of five herbs were retrieved by using traditional Chinese medicine systems pharmacology (TCMSP) and screening literature. Then, genome explanation databases (OMIM and GeneCards) were used to acquire targets related to CHD. The protein-protein interaction (PPI) network was built using the mutual targets filtering genes through protein interaction. Next, a network diagram could be established with the help of Cytoscape 3.8.2. And, STRING platform was used to construct a protein interaction network. Finally, GO and KEGG analyses were analyzed to further elucidate biological process enrichment.Results: After the screening, 36 active ingredients and 202 related targets in WYS in addition to 952 disease-related targets were acquired. A total of 37 key targets including AKT1, ESR1, and EGFR were screened in the PPI network. These targets were mostly concentrated on the transmembrane receptor protein tyrosine kinase signaling pathway, cellular response to growth factors stimulus, and response to growth factor. The KEGG enrichment demonstrated that the MAPK signaling pathway, P13K/AKT signaling pathway, Ras signaling pathway, and other corresponding signaling pathways were closely related to CHD.Conclusions. WYS plays a significant role in the treatment of CHD. And this study provides a novel approach to disclose the therapeutic mechanisms of WYS on CHD.
Highly palatable and hypercaloric chronic intake of food and soft drink leads to disruption in the normal dietary pattern in rats
Authors: Hagen Martine Elisabeth Kienzle , Macedo Isabel Cristina , Thoen Rutiane Ullmann , Fernandes Rafael Oliveira , Goularte Jeferson Ferraz , Sanvitto Gilberto Luiz , Torres Iraci L.S. 
Published: April 2021

Abstract
Ultra-processed food consumption, which is highly palatable, rich in fat, sugar, and salt causes uncontrolled food intake and has contributed to a sharp increase in obesity worldwide. This study evaluated the effects of soft drink and/or ultra-processed food intake on eating behavior and metabolic parameters in rats fed with a cafeteria diet. Male Wistar rats were divided into six groups. 1) CON: standard chow and water; 2) CD: cafeteria diet, standard chow, and water; 3) CS: caloric soft drink, standard chow, and water; 4) NCS: non-caloric soft drink, standard chow, and water; 5) CD + CS: cafeteria diet, caloric soft drink, standard chow, and water; and 6) CD + NCS: cafeteria diet, non-caloric soft drink, standard chow, and water. The cafeteria diet intake resulted in higher energy consumption (30% increase on average), a 450% increase in lipid consumption, and a 50% reduction in protein intake, which contributed to a 60% increase in body weight relative to the controls. This diet increased the metabolic risk, as observed in the Homeostasis Model Assessment changes in the CD groups. The CD + NCS group presented a lower liposomatic index, which may be related to the lower insulinogenic effect of caffeine contained in the soda, and 17% carbohydrate reduction compared to the CD + CS group. This result suggests that caffeine consumption in non-caloric soda may help prevent obesity associated with the sucrose's absence. However, it is necessary to compare the effects of the sugary drink intake with those of the artificially sweetened consumption, caffeine-free drinks associated with a cafeteria diet. No Level of evidence: animal study.
Intraoperative Chemotherapy for Leptomeningeal Metastases After Ommaya Placement
Authors: Omofoye Oluwaseun Adeola , Yu John S , Chu Ray M 
Published: April 2021

Abstract
IntroductionThere is a wide variety in the timing of the first intraventricular chemotherapy dose after Ommaya reservoir placement. Given the rapid nature of leptomeningeal metastasis, it is important to avoid any delays in treatment in order to have the optimal therapeutic benefit. We present the first series of immediate intraoperative intraventricular infusion of chemotherapy after Ommaya placement.MethodsA single-institution, retrospective review of twenty patients who underwent surgical placement of an Ommaya reservoir from 2012 to 2020 and had intraoperative infusion of chemotherapy was conducted. Inclusion criteria consisted of patients 18 years and older with a diagnosis of leptomeningeal metastases, central nervous system lymphoma or leukemia. Outcomes such as leukoencephalopathy, wound healing, intracranial hemorrhage, catheter malfunction, Ommaya days, mortality, and other complications were reviewed.ResultsThe mean patient age was 55.1 years and the most common diagnosis was breast cancer (40%). All catheters were placed into the ventricular system, and there were no wound healing complications, infections or symptomatic leukoencephalopathy. Intraventricular chemotherapy was administered for a total of 201 cycles and a mean of 10 times per patient. The number of Ommaya days ranged from 7 to 2177, with a mean of 326.5 days, and 30-day mortality was 10%.Conclusions Ommaya reservoirs are effective intraventricular delivery mechanism for chemotherapy in patients with leptomeningeal metastases. Endoscopy-assisted placement of Ommaya catheters provides a real-time, visual confirmation of adequate placement. Immediate intraoperative intraventricular infusion of chemotherapy after Ommaya placement is safe, effective, and may increase efficiency in time to treatment for patients.
Prevention of Sexually Transmitted Infections: Level of Knowledge Among Freshmen of Four Italian Universities
Authors: Cegolon Luca , Bortolotto Melania , Bellizzi Saverio , Cegolon Andrea , Pichierri Giuseppe , Mastrangelo Giuseppe , Xodo Carla 
Published: April 2021

Abstract
Background. With the aim to inform potential SHE programs in Italian schools, we run a survey on 4,552 freshmen of four different Italian universities (Padua, Palermo, Bergamo and Milan) to investigate their level of SHK on STI prevention and associated factors. Methods. The survey was conducted with an anonymous self-reported paper questionnaire administered during teaching lectures to university freshmen of four Italian universities and different undergraduate courses. Results. The final number of freshmen surveyed was 4,552 (97.9% response rate), 1,842 (40.5%) from the university of Padua; 1,743 (38.3%) from the university of Palermo; 667 (14.7%) from the university of Bergamo and 300 (6.6%) from the university of Milan. The mean age of respondents was 21.4 2.2 years and most of them (70.3%) were females. An overall low STI knowledge among sample students was found. Only 28% respondents knew the most effective contraceptives to prevent STI (i.e. condom and sexual abstinence), with a slightly higher prevalence of correct answers among females (31.3%) than males (25.8%). Students with history of STIs reported referring mostly to their GP (38.1%), rather than discussing the problem with a family member (15.1%), booking an appointment with a consultant gynecologist (13.1%), discussing with their partner (13.1%) or with an experienced friend (12.5%). At multivariable analysis significantly lower levels of STI knowledge was found among males, students of the university of Palermo, those who completed a vocational secondary school education and non-Italian nationals. Categories with significantly less SHK on the most effective contraceptives to prevent STIs included males, students of the university of Palermo, students of educational sciences, students of economics/political sciences, those of foreign nationality, those with two siblings and those whose fathers had a lower educational level. Conclusions. The above findings provided indirect information on the standard of SHE delivered in Italian schools and call for the development of tailored multi-agency SHE programs involving several actors. In addition to school-based SHE programs, primary care services dedicated to sexual health should be strengthened in Italy.