bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Focal Image for Solar Parabolic Dish Concentrator With Square Facets – An Analytical Model
Authors: Kopalakrishnaswami Arjun Singh , Loni Reyhaneh , Najafi Ghoalmhosein , NATARAJAN SENDHIL KUMAR 
Published: December 2021

Abstract
Solar parabolic dish concentrator is one of the high-temperature applications of more than 400 °C for thermal and electrical power generation. In the solar parabolic dish concentrator, the arrangement of reflectors over the surface area is the significant factor for effective concentration of solar radiation. Also, focal image is one of the most influencing parameters in the design of receiver. Among the various reflectors, the square shaped reflectors (facets) are comparatively effective in converging the incoming radiations to attain better focal image. In this regard, an attempt has been made to predict the focal image diameter of a solar parabolic dish concentrator with a square facet of different influencing parameters using a novel mathematical model. The influencing parameters considered for the study are aperture diameter, rim angle, and facet length of the dish concentrator. Based on the proposed model, the focal image dimension and aperture area of a solar parabolic dish concentrator with square facets can be predicted accurately for efficient design of a solar parabolic dish collector system. Finally, the proposed model is validated with the experimentally obtained focal image diameter and it is observed that the predicted result is in good agreement with the experimental one. Thus, the proposed model can be effectively used for the design of parabolic dish system for sustainable development.
Control Efficiency and Mechanism of Spinetoram Seed-Pelleting Against the Striped Flea Beetle Phyllotreta Striolata
Authors: Xiong Tengfei , satyabrata Nanda , Jin Fengliang , Lin Qingsheng , Feng Xia 
Published: December 2021

Abstract
The striped flea beetle (SFB, Phyllotreta striolata) is an important pest of the cruciferous crops in Asia. SFB is regarded as the most destructive pest of cruciferous crops in China due to the severe crop loss and frequent infestation incidents. As no SFB resistant cultivar is available at present, therefore, application of insecticides is the primary method of SFB control. On the contrary, the exploitation of chemical insecticides causes severe environmental issues and is not cost-effective. The use of a seed-pelletized coating of spinetoram effectively reduced SFB feedings on the flowering cabbage seedlings, whereas in combination with the insect-proof net, it controlled the SFB infestation throughout the cabbage growth period. The analysis of the pesticide residues in soil and different cabbage parts indicated the degradation dynamics of spinetoram. Furthermore, estimation of the half-life of spinetoram revealed that via seed-palletized application spinetoram half-life was found to be 2.82 days in soil, 4.21 days in the root, 5.77 days in the stem, and 3.57 days in the leaf, respectively. Both the lower pesticide residues and the half-life of spinetoram in soil and cabbage parts suggested it to be a promising environment and food-safe pesticide in controlling SFB. Moreover, the seed-pelletized coating ensured a sustainable release of spinetoram that can reduce the pesticide application frequency and be cost-effective and pocket-friendly for the farmers.
Comparative Transcriptome Analysis of the Roots and Leaves of Bupleurum Chinense DC. Seedlings Under Drought Stress
Authors: Fan Ya , Geng Yannan , Cao Hailu , Liu Li , Zhang Quanfang , Yan Sun , Kan Du , Wang Ping , Gao Demin 
Published: December 2021

Abstract
Background
drought stress is one of the important environmental factors affecting the quality and yield of medicinal materials, and is the main factor restricting the field production of Bupleurum chinense. B. chinense seedlings sensitive to low moisture, but there are few reports on the molecular mechanism of B. chinense seedlings under drought stress. Therefore, the transcriptome of the leaves and roots of B. chinense seedlings before and after drought were analyzed by Illumina sequencing technology and bioinformatics analysis.
Results
a total of 59.82 GB of clean data was obtained, and the unigenes were compared with Nr, Swissprot, String, GO, KEGG, and Pfam databases. Under drought stress, 3,737 and 6,816 differentially expressed genes (DEGs) were identified in leaves and roots of B. chinense, respectively. The obtained DEGs from leaves and roots were classified into 37, and 36 GO terms and were involved in 222 and 253 KEGG pathways, respectively. SSR analysis were obtained identified 33,728 loci, wherein dinucleotides accounted for the largest proportion. Genes involved in diterpenoid and unsaturated fatty acid biosynthesis were significantly over-expressed in roots under drought stress, suggesting these two cellular processes underpin the adaptation and resistance of B. chinense seedlings to drought stress.
Conclusions
the results provided a theoretical basis for further identification of the molecular mechanism of drought resistance and breeding of drought resistance varieties of B. chinense.
Optimal Characterization of 28 GHz Bowtie Antenna for 5G Applications
Authors: ULUSLU AHMET 
Published: December 2021

Abstract
Here, the selection of the design parameters of the bowtie patch antenna (BPA) for 5G applications is presented as a multidimensional, multi-purpose design optimization problem. The operating frequency of the proposed antenna is 28 GHz, which is the standard for millimeter waveband and 5G technologies. In order to overcome this difficult design optimization, a new, fast and powerful optimization algorithm was used by modified the non-dominant sorting genetic algorithm (NSGA)-III and optimal characterization of the microwave antenna design was obtained. It is assumed that the optimal characterization gives the best solution for the determined cost function, among the possible solutions within the specified range. The superiority of the proposed method has been proven by comparing it with similar types of algorithm. The antennas in the results found have good performance operating at 28 GHz, with a return loss of up to –49 dB, a gain of around 1.96 dB, good directivity, and the radiation pattern of the proposed antenna has a good match over the required frequency. Therefore, the proposed design can be used in 5G devices. As a whole, the proposed design optimization process is an efficient, fast and reliable solution for all antenna design problems.
Bismuth Subgallate as a Local Hemostatic Agent
Authors: Rahajoe Poerwati Soetji , Hasan Cahya Yustisia , Pranoto Amelia Elizabeth 
Published: December 2021

Abstract
Background: Patients on clopidogrel increased bleeding risk after surgery. This drug prolonged bleeding time, increased bleeding volume and induced secondary bleeding because its active metabolite inhibited platelets aggregation and interfered with haemostatic plug stabilization. Conventional methods, such as pressing sterile gauze on the surgery site, showed less effective to stop bleeding in patients on clopidogrel. This research aims to prove the haemostatic effect of bismuth subgallate both on normal and delayed platelet aggregation due to clopidogrel.Methods: Twenty-eight Wistar rats were equally and randomly administered with clopidogrel (10 mg/kgBW) or NaCl 0.9% (saline) via oral gavage. After anesthetizing, we amputated transversely their tail 10 mm from the distal tip. Bleeding after amputation was controlled with pressing gauze soaked in saline or bismuth subgallate solution. After 60 seconds, bleeding assays (bleeding time, bleeding volume, and secondary bleeding) have been observed, recorded, and analysed both in normal and clopidogrel groups.Results: Clopidogrel groups had significantly longer bleeding time, greater bleeding volume, and had more secondary bleeding rather than saline groups (p <.05). Using bismuth subgallate as local haemostatic agent decreased bleeding time and bleeding volume significantly (p <.05) both in normal and clopidogrel groups. Conclusions: Bismuth subgallate has a haemostatic effect on both clopidogrel and normal rat tail bleeding models.
Hippo Pathway-related Genes Expression Is Deregulated in Myeloproliferative Neoplasms
Authors: Cacemiro Maira da Costa , Cominal Juçara Gastaldi , Pereira Luiz Miguel , Berzoti-Coelho Maria Gabriela , Berbel Giovana Michelassi , Baroni Luciana , Malta Tathiane , Tognon Raquel , Nunes Natalia de Souza , Souto Elizabeth Xisto , de Figueiredo-Pontes Lorena Lobo , Yatsuda Ana Patricia , de Castro Fabiola Attié 
Published: December 2021

Abstract
Myeloproliferative neoplasms (MPN) are hematological disorders characterized by increased proliferation of precursor and mature myeloid cells. MPN patients may present driver mutations in JAK2, MPL, and CALR genes, which are essential to describe the molecular mechanisms of MPN pathogenesis. Despite all the new knowledge on MPN pathogenesis, many questions remain to be answered to develop effective therapies to cure MPN or impair its progression to acute myeloid leukemia. The present study examined the expression levels of the Hippo signaling pathway members in patients with polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), as well as the role that they play in disease pathogenesis. The Hippo pathway is a tumor suppressor pathway that participates in the regulation of cell proliferation, differentiation, and death. Our main findings were: (i) expression of tumor suppressor genes from Hippo pathway were downregulated and seemed to be associated with cell resistance to apoptosis and increased proliferation rate; and (ii) Hippo pathway-related gene expression was associated with mutation status in ET and PMF patients. Therefore, the decreased expression of Hippo pathway-related genes may contribute to the malignant phenotype, apoptosis resistance, and cell proliferation in MPN pathogenesis.
Fatigue Strength Evaluation of Welded Structure on Aluminum Alloy Car Body Based on Multi-Axial Stress
Authors: Shangguan Yiming , Wang Wenjing , Yang Chao , He Anrui 
Published: December 2021

Abstract
With the continuous development of the subway, the demand for its safety and stability is getting higher and higher. It is of great significance to accurately evaluate the fatigue life of the carbody to ensure the subway's safe operation. In this paper, the finite element model of a subway head carbody was established, and the fatigue strength of the welded structure on the carbody was evaluated based on Multi-axial stress. The local coordinate system was defined according to the geometrical characteristics of the welds. Local stresses perpendicular and parallel to the weld seam were obtained to calculate the stress ratio, stress range, and allowable stress value corresponding to the stress component. According to the joint fatigue resistance, the components of the degree of utilization and comprehensive degree of utilization are calculated to evaluate the structural fatigue strength under the survival rate of 97.5% and load cycles of 10 7 . The evaluation of the fatigue strength of the pivotal weld joints shows that the fatigue strength of the aluminum alloy carbody meets the design requirements, the weld of the carbody has a strong ability to resist fatigue damage. The fatigue strength of the weld is mainly affected by the normal stress component, while the shear stress has little effect on the fatigue strength of the structure. In addition, compared with the filleted weld joint and the butt-welded joint, the normal stress parallels to and perpendicular to the weld direction and shear stress have the greatest effect on the lap-welded joint. Meanwhile, the comprehensive degree of utilization of the lap-welded joint is the largest at 0.49. The introduction of multi-axial stress for the fatigue strength evaluation is beneficial when considering the material utilization degree in multiple structural directions. This research results provide a reference for fatigue strength evaluation of subway carbody's welded structure.
Assessment of Veterinary Extension Service and Public Perception on Major Infectious and Zoonotic Disease at Robe Veterinary Clinic of Bale Zone, Oromia, Ethiopia
Authors: Neja Sultan Abda , Adam Amir 
Published: December 2021

Abstract
Background

A survey-based cross-sectional study was conducted from April to August 2021 to assess veterinary extension service and public perception on major infectious and zoonotic diseases at Robe veterinary clinic. 384 animal owners who brought their animals for treatment were interviewed.
Results

The result indicated that 70.8% of respondents had no veterinary extension service. The majority (88%) had no access to the veterinary consultant. Although 51.6% of them used AI, only 7.8% of them know how to detect estrous. The most frequently known zoonotic diseases were Rabies (96.6%) followed by Anthrax (33.07%), and Tuberculosis (4.2%). Out of the 96.6% of respondents that replied to know about Rabies, 51.8% of them mentioned the disease can be transmitted only through dog biting while 14.8% of them say through biting and contact with saliva. Likewise, only 43.3% of respondents perceive that Anthrax is transmitted through ingestion, whereas 85% of them mentioned Tuberculosis can be transmitted from animal to humans only through inhalation. Although most animal owners prefer early treatment of animals in the veterinary clinic, there are also practices of traditional animal treatment. Remarkable only 16.4% and 24.7% of respondents know the importance of vaccination and slaughterhouse respectively. 93.8% and 94.8% of them still consume raw milk and meat respectively.
Conclussion

: The finding indicates that veterinary extension services are a neglected venture; the knowledge, attitude, and practice on prevention and control of major infectious and zoonotic diseases are still insufficient. Therefore there should be multidisciplinary one-health approach in educating the community about infectious and zoonotic diseases.
Learning Curves of Robotic Spine Surgery in a System Lack of Active Perception: Potential Roles of Teamwork and Unmet Needs
Authors: Su Yu-feng , Tsai Tai-Hsin , Kuo Keng-Liang , Wu Chieh-Hsin , Tsai Cheng-Yu , Lu Yen-Mou , Hwang Shiuh-Lin , Lin Pei-Chen , Lieu Ann-Shung , Lin Chih-Lung , Chang Chih-Hui 
Published: December 2021

Abstract
Background: The aim of this study was to investigate the learning curve of robotic spine surgery quantitatively with the well-described power law of practice.Methods: Kaohsiung Medical University Hospital set up a robotic spine surgery team by the neurosurgery department in 2013 and the orthopedic department joined the well-established team in 2014. A total of 150 cases and 841 transpedicular screws were enrolled into 3 groups: the first 50 cases performed by neurosurgeons, the first 50 cases by orthopedic surgeons, and 50 cases by neurosurgeons after the orthopedic surgeons joined the team. The time per screw and accuracy by each group and individual surgeon were analyzed.Results: The time per screw for each group was 9.56±4.19, 7.29±3.64, and 8.74±5.77 minutes respectively. The accuracy was 99.6% (253/254), 99.5% (361/363), and 99.1% (222/224), respectively. The first group took significantly more time per screw, but without significance on the nonlinear parallelism test. Analysis of 5 surgeons and their first 10 cases of short segment surgery showed the time per screw by each surgeon was 12.28±5.21, 6.38±1.54, 8.68±3.10, 6.33±1.90, and 6.73±1.81 minutes. The first surgeon who initiated the robotic spine surgery took significantly more time per screw and the nonlinear parallelism test also revealed only the first surgeon had a steeper learning curve. Conclusions: This is the first study to demonstrate that differences of learning curves between individual surgeons and teams. The roles of teamwork and the unmet needs due to lack of active perception are discussed.
A Simplified Gibson Assembly Method for Site Directed Mutagenesis Using Non-Gibson Primers
Authors: Olszakier Shunit , Berlin Shai 
Published: December 2021

Abstract
Background: Site-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution. Although many SDM methods have been developed, methods that increase efficiency and versatility of this process remain highly desired. Method: We present a versatile and simple method to efficiently introduce a variety of mutation schemes using the Gibson-assembly without the need for unique Gibson primers. The method entails use of standard SDM primers (shorter and completely overlapping in sequences in contrast to Gibson primers) that are separately employed with common primer (~25 bps long) for amplification of fragments flanking the site of mutagenesis, followed by rapid amplification of the Gibson-assembled product for added visualization and sequencing steps for ensuring high success rates.Results: We find that assembly of the fragments via the Gibson reaction mixture is attainable within as short as 15 minutes, despite the need for extensive digestion of the DNA (by exonuclease) past the entire SDM primer sequence (to expose non-clashing overlap between the fragments). We also find that the amount of the assembled Gibson product is too low to be visualized and assessed on standard agarose gel. We thereby introduce a short amplification step (by use of the same short primers initially employed) to 1) easily resolve whether the product (only the correct size can yield a product) has been obtained, and 2) for isolation of product for DNA-sequencing (to assess whether mutation(s) have been introduced). No other SDM method enables assessment of mutagenesis prior completion of the process. Conclusion: We employ our approach to delete, replace, insert, and degenerate sequences within target DNA sequences, specifically in DNA sequences that proved very resistant to mutagenesis by multiple other SDM methods (standard and commercial). The entire protocol spans only four days, requires minimal primers sets (as well as can be used with most in-house primers) and provides very high yields and success rates (>98%).
Link Between Unmet Need and Economic Status in Bangladesh: Gap in Urban and Rural Areas
Authors: Shabuz Zillur Rahman , Haque Md. Ershadul , Islam Md. Kawsarul , Bari Wasimul 
Published: December 2021

Abstract
Background: Unmet need for family planning (FP) is a core concept in designing FP programmes and reduction of unmet need for FP can improve reproductive and maternal health services. Bangladesh is still away from achieving the target regarding unmet need for FP. This study aimed to explore the composite effect of economic status and place of residence on unmet need for FP among currently married women of reproductive age in Bangladesh after controlling the effect of other selected covariates.Methods: The study used the data extracted from the Bangladesh Demographic and Health Survey (BDHS) 2017-2018, which is a nationally representative survey implemented using a stratified two-stage cluster sample design. A total of 13031 currently married women of reproductive age were included in the final analysis. Binary logistic regression model has been employed to identify the factors influencing the unmet need for FP. Model-I investigated the effect of composite variable place-wealth on unmet need for FP and Model-II examined the effect of place-wealth on unmet need for FP after adjusting for the effect of other selected covariates. The Odds Ratios with p-values were reported to identify significant covariates.Results: The rate of unmet need for FP was 15.48%. The composite factor of economic status and place of residence had significant influence on unmet need for FP in both models. Generally, rural women were significantly more likely to have unmet need for FP than their urban counterparts. In particular, women from rural areas and belong to rich families had the highest likelihoods of unmet need for FP. The other selected covariates also had significant influence on unmet need for FP. Conclusion: This study shows that rural women had higher odds of unmet need for FP than urban women. The Government of Bangladesh (GoB) should take necessary actions to motivate women to use contraceptive specially the women who are residing in the rural areas.
Presepsin and platelet to lymphocyte ratio predict the progression of septic subclinical acute kidney injury to septic acute kidney injury and the prognosis of subclinical septic acute kidney injury patients: a pilot study
Authors: Shimoyama Yuichiro , Umegaki Osamu , Kadono Noriko , Minami Toshiaki 
Published: December 2021

Abstract
This study aimed to determine whether presepsin can predict the progression of septic subclinical acute kidney injury (AKI) to septic AKI among intensive care unit (ICU) patients.
Presepsin values were measured immediately after ICU admission (baseline) and on Days 2, 3, and 5 after ICU admission. Glasgow Prognostic Score, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio (PLR), Prognostic Index, and Prognostic Nutritional Index were measured at baseline. Presepsin values and these indices were compared between septic AKI and septic subclinical AKI patients.
There were 38 septic AKI patients and 21 septic subclinical AKI patients. Receiver operating characteristic curve analyses revealed the following cut-off values for AKI (relative to subclinical AKI): 708.0 (pg/ml) for presepsin on Day 1 (AUC, 0.69; sensitivity, 82%; specificity, 52%), 1283.0 (pg/ml) for presepsin on Day 2 (AUC, 0.69; sensitivity, 55%; specificity, 80%), and 368.66 for PLR (AUC, 0.67; sensitivity, 71%; specificity, 62%). Multivariate logistic regression analyses revealed PLR to be a predictor of septic subclinical AKI (odds ratio, 1.0023; 95% confidence interval, 1.0000-1.0046; p=0.046).
Presepsin and PLR predicted the progression of septic subclinical AKI to septic AKI and the prognosis of subclinical septic AKI patients.
Poor Tricuspid Regurgitation Signal on Echocardiogram: How Does It Correlate with Pulmonary Artery Pressure as Measured by Right Heart Catheterization?
Authors: Aziz Mohammad , Romero Steven , Price Matthew , Mohan Rajeev 
Published: December 2021

Abstract
BackgroundTricuspid Regurgitation (TR) gradient on echocardiogram is used to approximate pulmonary artery pressure (PAP) on echocardiography. A common dilemma is encountered when PAP measurement is indeterminate due to poor TR signal. We hypothesized that patients with poor TR signal would be unlikely to have pulmonary hypertension (PH) on right heart catheterization (RHC). MethodsWe performed a retrospective analysis of 141 patients who underwent RHC and had a corresponding echocardiogram showing poor TR signal within 2 months of RHC. A cutoff of 25 mm Hg was used as the upper limit of normal to define PH. ResultsFifty percent of patients had mean PAP (mPAP) greater than 25 mm Hg. 82% of values were 35 mm Hg or below. ConclusionsPoor TR signal does not rule out PH but may indicate lower likelihood of severe PH.