bluuebunny/crossref_metadata_2025_split huggingface dataset:
Secondary amenorrhea in girls: genealogical and cytogenetic features
Authors: Bagatska N. V. , Nefidova V.E. , Novohatska S.V. 
Published: October 2021

Aim. To determine genealogical and cytogenetic features in girls aged 12-17 years with secondary amenorrhea. Methods. The analysis of pedigrees was conducted in 25 families of girls with secondary amenorrhea (main group) and in 25 families of healthy girls in the laboratory of medical genetics of SI "ICAHC NAMS". Cytogenetic analysis was carried out in the blood lymphocytes of the girls of the main and control groups in vitro. The control group consisted of 25 healthy peer girls with a regular menstrual cycle without somatic pathology. The data obtained were analyzed statistically using the Student's t-test in Excel programs. Results. The hereditary burden on non-inflammatory (hormone-dependent) gynecological diseases was found in 60.0% of families, in 86.6% of cases – along the maternal line, in 6.7% – along the paternal line, in 6.7% – on both lines at the same time; 40.0% of girls had no hereditary burden. The total incidence of gynecological (non-inflammatory) diseases among relatives of three degrees of kinship was 13.6%, which was almost three times higher than the frequency in relatives of healthy girls (5.1%, p < 0.001). Cytogenetic analysis conducted in girls of the main group showed an increase in both the overall level of chromosomal disorders (6.2%), and their individual types (3.2%. 3.0%, 1.56%) compared to the frequency in healthy girls. Conclusions. Family accumulation of gynecological (non-inflammatory) diseases in the pedigree of girls with secondary amenorrhea has been established. Cytogenetic features in the blood lymphocytes of sick girls are revealed compared to healthy peers.
Keywords: girls, pedigrees, cytogenetic indices, secondary amenorrhea.
Dynamics of natural selection in two generations (on the example of the population of the Kirovograd region)
Authors: Kovleva K. K. , Kozak N.A. 
Published: October 2021

Aim. In connection with the success of modern medicine, the pressure of natural selection in various civilized human populations is weakening, which leads to the accumulation of a genetic load. The purpose of this work was to trace the change in the intensity of natural selection among population of the Kirovograd region in two successive generations. Methods. The collection of material was carried out in 2020 and 2021. Anonymous questionnaires were conducted and medical histories of women of post-reproductive age of the Kirovograd region were studied. The first generation included 40 women born in 1937–1959; the second generation consists of 273 women born in 1960–1981. Results. The total selection index was 0.27 in the first generation, and 0.37 in the second generation. The percentage of women who have not had pregnancies increased from the first generation to the second from 2.5 to 3.7, respectively. Conclusions. The index of total selection in the Kirovograd region population for one generation increased by almost one and a half times (from 0.27 to 0.37), as well as the index of differential fertility (from 0.25 to 0.35).
Keywords: reproductive characteristics, Kirovograd population, Crow's index, selection, generations.
KIR-HLAC genotyping in married couples with early reproductive losses of unknown genesis
Authors: Sosnina K. O. , Zastavna D.V. , Terpyliak O.I. 
Published: October 2021

Aim. KIR-HLAC genotyping in married couples with early idiopathic pregnancy loss. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes was analyzed and the frequency of KIR genotypes in women with early reproductive losses was established. The most common (77.78 %) was the AB genotype, 20.37 % had the AA genotype, and 1.85 % had the BB genotype. HLAC genotyping of couples with regular early reproductive losses showed the C1/C2 genotype of the HLAC gene in 40.74 % of women and 44.44 % of men. The frequency of C1/C1 genotype in women was 27.78% versus 38.89 % in men. The C2/C2 genotype of the HLAC gene was detected in 31.48 % of women and 12.96 % of men. According to the results of KIR-HLAC analysis of genotyping of married couples with early reproductive losses, a high/significant risk of reproductive losses of immunological genesis was found in 55.56 % of cases. Conclusions. KIR-HLAC genotyping is a genetic test that allows to assess the risks of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy.
Keywords: early reproductive losses, KIR, HLAC.
Anxiety and depression: population distribution and family associations
Authors: Etkalo E. N. , ATRAMENTOVA L.A. 
Published: October 2021

Aim. The aim of the study was to characterize the population distribution of psychiatric phenotypes according to anxiety and depression. Methods. The level of anxiety and depression was assessed with the HADS Hospital Anxiety and Depression Scale test. Students in grades 9-11 of secondary schools in Kharkiv, as well as their parents, were tested. We tested 306 persons from 115 families. Results. The frequency of depression among men in the Kharkiv population is 9%, among women 11%, among boys 15%, among girls 22%; the frequency of increased anxiety among men is 14%, among women 22%, among boys 19%, among girls 44%. There is a positive relationship between anxiety and depression in older men and women, expressed by the association coefficient in men rDT =0.45, in women rDT =0.79. The relatives revealed a similarity in psychological types, described by the association index for depression in mother/daughter pairs r = 0.40, mother/son r = 0.03, father/daughter r = 0.20, father/son r = 0.40; for anxiety: mother/daughter r = 0.15, mother/son r = 0.05, father/daughter r = 0.24, father/son r = 0.01. Conclusions. Individuals of the younger generation are more likely to have signs of anxiety and depression than those of their parents' generation. The parent-offspring phenotype relationship is stronger in same-sex couples compared to opposite-sex couples.
Keywords: depression, anxiety, population distribution, family analysis.
Study of the intrapopulation polymorphism of Trifolium repens L. from Lanivtsi under the anthropogenic load of various intensity
Authors: Kryzhanovska M. M. , Holub N. Ya. , Prokopiak M. Z. , Holinei H. M. 
Published: October 2021

Aim. To study the phenotypic polymorphism of Trifolium repens L. populations growing under various anthropogenic load. Methods. The quantitative calculation of the leaves of the white clover by the presence or absence of the white leaf mark; the identification of the phenotype and genotype of the plant according to the pattern of the white leaf mark; the analysis of the phenotypic diversity and the study of the percentage of rare phenotypes; the calculation of the index of the phenotypes ratio. Results. 4 phenotypes were identified in the pasture area. The most common of them were the plants without the white mark with a frequency of 56.2 %. The plants with a full spot accounted for 27.5 %, with a spot with a gap – 15.4 %, with a central spot – 2.2 %. Heterozygous plants were absent. In the central part of the city, 7 genotypes were identified. Among these genotypes there were the significant decrease of the recessive homozygotes (by 43.6 %) and the increase of the frequency of VV (by 15.2 %) and VHVH (by 21.2 %) genotypes. Heterozygotes accounted for 1.3–3.0 %. The intrapopulation diversity in this territory was the highest (5.1) among the studied areas. In the population growing near Ternopil-Lanivtsi road, we identified 6 genotypes. The plants without spot (vv) and with the full spot (VV) were found with identical frequency of 34–35 %. The plants with a full high spot (VHVH) were about 20.4 %. Other phenotypes amounted to 10 %. Conclusions. In the populations located in ecologically polluted and anthropogenically loaded areas, the sets of alleles expand and the specific phenotypes appear under the influence of the mutation processes and natural selection. In the population without the anthropogenic load the decrease of the polymorphism and the increase of the frequency of individual genotypes (vv, VV) were observed.
Keywords: Trifolium repens L., leaf phenotype, intrapopulation polymorphism, multiple allelism, bioindication, anthropogenic load.
Un acteur entre deux réseaux antagoniques dans la Martinique de la Libération : la brève et tragique administration du gouverneur Ponton (1943-1944)
Authors: Lavenaire Maël 
Published: October 2021

Arrivé en Martinique dans le contexte de la Libération, entre août 1943 et juillet 1944, le gouverneur Georges-Louis Ponton se distingue par la construction d’un réseau, aussi bien politique que culturel tout à fait inédit dans l’histoire de la haute fonction publique de cette colonie. En se rapprochant des intellectuels noirs martiniquais et du milieu socialiste, le réseau qu’il met en place est à l’image de sa vision politique « progressiste ». Très rapidement, son objectif est de permettre à la Martinique d’être érigée au statut de département français. Néanmoins, ce jeune gouverneur qui doit répondre également aux attentes fortes de la plantocratie des blancs créoles martiniquais se retrouve pris au piège d’une société coloniale qu’il ne connaît pas assez et dont les antagonismes socio-raciaux qui datent de plusieurs siècles, sont particulièrement intenses.
Analog Resources and Digital Limitations
Authors: Perabo Lyonel 
Published: October 2021

Following the completion of a bachelor in History, started in France but completed in Tromsø as an exchange student, I started to develop an interest for Scandinavian History and culture, which translated in me moving to Iceland to enroll in the Old Norse Religion MA program at the university of Iceland in 2013. After graduation, three years later, I began the life of an unaffiliated early-career scholar eager to make use of my newly-acquired knowledge. Since then, I have met with a number of obstacles related to access to scientific publications and source material, as well as discovered and developed ways around such problems.
Working largely outside the framework of a higher-education establishment, my academic experience has so far been characterized by the mixing of traditional research methods and resources with more informal approaches. Finding primary sources, always a capital task for researchers of older History, is the perfect example of how contemporary Old Norse scholars combine long-established resources such as scholarly editions and manuscript transcription with less well-established web-based material such as amateur translations and commentaries.
Online support and networking groups, largely operating via social media pages also do play an important role in facilitating collaboration between scholars, wanna-be scholars, and other enthusiasts, as well as making less-accessible resources more widely-distributed. One such example of collaborative internet-based academic project is the current Old Norse translation network I have been a part of since last year. Gathering individuals currently or formerly employed in academia, as well as enthusiastic amateurs and prospective academics, it makes for a relevant case study. This can be used to demonstrate how contemporary Old Norse scholars must operate in a hybrid field where the ever-growing amount of online resources must nevertheless be critically balanced with traditional published sources in order to conduct research.
Multilingualism in Scholarly Communication
Authors: Leão Delfim 
Published: October 2021

The results of the study and the survey conducted on behalf of the OPERAS-P project (Task “Innovative Models of Bibliodiversity in Scholarly Publications”) were concluded in June 2021 and sought to achieve the following objectives:

to prepare a theoretical background to discuss the use of multilingualism in scholarly communication;
to identify, analyse, and understand the innovative dynamics of working practices and knowledge-sharing within linguistically diverse scholarly contexts and research networks;
to identify and analyse the motivations behind these practices (questionnaires/focus groups – how tools may answer to needs);
to formulate recommendations/guidelines for OPERAS and other stakeholders regarding the future implementation of a service aimed at enhancing multilingualism;
to prepare the conceptual design of a platform prototype for a shared translation service at the scholarly communication level (involving publishers, translators, and researchers).

This presentation approaches the most important stages of the work done, as well as the main findings and the challenges they pose for future developments and their implementation.
Enabling smaller independent publishers to participate in Open Access transformative arrangements
Authors: Estelle Lorraine 
Published: October 2021

See RECORDING.
Independent Society Publishers and Academic Publishers would like to enable Open Access transformative agreements, but find it difficult to do this at scale, especially when each library consortia requires different licence agreements, data and workflows. This project is to create shared principles, a model licence, a data template and minimum workflows, so that small publishers, libraries and library consortia can then use them to more easily conclude Open Access agreements.
Identification and validation of a novel zinc finger protein-related gene-based prognostic model for breast cancer
Authors: Ye Min , Li Liang , Liu Donghua , Wang Qiuming , Zhang Yunuo , Zhang Jinfeng 
Published: October 2021

Background
Breast invasive carcinoma (BRCA) is a commonly occurring malignant tumor. Zinc finger proteins (ZNFs) constitute the largest transcription factor family in the human genome and play a mechanistic role in many cancers’ development. The prognostic value of ZNFs has yet to be approached systematically for BRCA.

Methods
We analyzed the data of a training set from The Cancer Genome Atlas (TCGA) database and two validation cohort from GSE20685 and METABRIC datasets, composed of 3,231 BRCA patients. After screening the differentially expressed ZNFs, univariate Cox regression, LASSO, and multiple Cox regression analysis were performed to construct a risk-based predictive model. ESTIMATE algorithm, single-sample gene set enrichment analysis (ssGSEA), and gene set enrichment analyses (GSEA) were utilized to assess the potential relations among the tumor immune microenvironment and ZNFs in BRCA.

Results
In this study, we profiled ZNF expression in TCGA based BRCA cohort and developed a novel prognostic model based on 14 genes with ZNF relations. This model was composed of high and low-score groups for BRCA classification. Based upon Kaplan-Meier survival curves, risk-status-based prognosis illustrated significant differences. We integrated the 14 ZNF-gene signature with patient clinicopathological data for nomogram construction with accurate 1-, 3-, and 5-overall survival predictive capabilities. We then accessed the Genomics of Drug Sensitivity in Cancer database for therapeutic drug response prediction of signature-defined BRCA patient groupings for our selected TCGA population. The signature also predicts sensitivity to chemotherapeutic and molecular-targeted agents in high- and low-risk patients afflicted with BRCA. Functional analysis suggested JAK STAT, VEGF, MAPK, NOTCH TOLL-like receptor, NOD-like receptor signaling pathways, apoptosis, and cancer-based pathways could be key for ZNF-related BRCA development. Interestingly, based on the results of ESTIMATE, ssGSEA, and GSEA analysis, we elucidated that our ZNF-gene signature had pivotal regulatory effects on the tumor immune microenvironment for BRCA.

Conclusion
Our findings shed light on the potential contribution of ZNFs to the pathogenesis of BRCA and may inform clinical practice to guide individualized treatment.
Evaluation of the Variability of Drinking Water Quality within Bamenda Metropolis, North West Region, Cameroon
Authors: Ndah Anyang Gordin Bah , Ngwabie Ngwa Martin , Ayonghe Samuel Ndonwi 
Published: October 2021

Aims: The quality of drinking water within Bamenda metropolis was evaluated for it variability and suitability.
Place and Duration of Study: Twenty-two (22) samples were collected (11 in the dry season and 11 in the wet season) from 4 main drinking water network in Bamenda (public, community, non-distributed and private network).
Methodology: The samples were tested for physico-chemical and bacteriological parameters. American Society for Testing and Materials (ASTM) and Norme Française (NF) were the methods used to determine the organoleptic, natural structure, undesirable, toxic and bacteriological parameters of the different samples. Water Quality Index (WQI), Na/Cl ratio and hydrochemical facies were deduced from the physiochemical parameters.
Results: The findings indicate that water quality in Bamenda varies with seasons, location and sources. The pH of the study area was acidic with a higher dry season mean percentage of 52.6% against 47.4% for wet season. Turbidity showed amean percentage of 75: 25% for wet and dry season respectively. Wet season cations, showed abundance Ca2+ and Mg2+ while dry season showed Ca2+ and Na+. Bicarbonate and Chloride were the most abundant anions in both seasons but varied with seasonal concentrations. Bacteriological analysis identified faecal coliform in 3 dry season samples. Hydrochemical facies showed dominant of magnesium and bicarbonate for wet season samples while Sodium and Chlorine were dominant for dry season samples. Water Quality Index (WQI) ranged from 72 to 94 for the wet season and 84 to 100 for dry season.
Conclusion: Though the results for WQI were within the acceptable standard for drinking water, pH for 21 samples and turbidity of 6 samples were not within the Cameroon nor World Health Organisation (WHO) Standard for drinking water. It is important that drinking water be tested seasonally to ascertain the quality being consumed.
Evaluation of Crop and Soil Dynamics under Various Moisture Regimes and Moisture Conservation Techniques in Rice (Oryza sativa L.)
Authors: Kumar Deepak , Singh Adesh , Vivek . , Kumar Mukesh , Shahi U. P. , Singh Ramji 
Published: October 2021

Aim: The present investigation was conducted to validate the various moisture regimes in transplanted rice and to study the effect of different moisture conservation techniques on performance of rice and soil after harvest of crop.
Study Design: Experiment was laid out in split plot design (SPD).
Place and duration of Study: The present investigation was conducted during the kharif season of 2018 and 2019 at the Crop Research Centre of Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut (U.P.), India.
Methodology: The main factor consists of the moisture regimes viz. irrigation as CF (I1), at FC (I2) and at 25%DASM (I3), the sub factors consist of six moisture conservation techniques viz. control, application of wheat residues @ 5t/ha, Pusa hydrogel @7.5 kg/ha, seed treatment with PF-6, PF-2 and IRRI-1 @ 4g/kg seed. Observation on plant growth attributes viz., plant height and leaf area index were recorded at 30, 60 DAT and at harvest stage of the crop, while chlorophyll content was recorded at 30 and 60 DAT. The harvest index was calculated on the net plot area basis. Observation on soil dynamics viz., available NPK and OC were recorded both before and after harvesting of crop.
Results: Among different moisture regimes, the highest plant height, LAI and chlorophyll content was found under CF (I1) followed by FC (I2) during both the years. Among MCTs, the highest values of growth attributes and harvest index of rice were found with application of wheat residue @ 5 t/ha followed by Pusa hydrogel @ 7.5 kg/ha during both the years. Maximum value of harvest index was recorded under CF during first year, while it was highest under FC during 2019. Slight improvement in available nutrients were observed at 25% DASM treatment of moisture regimes and wheat residues @ 5 t/ha treatment of moisture conservation techniques over their other counterparts during both the years of experimentation.
Conclusion: On the basis of study, it may be concluded that irrigation should be scheduled at FC (2-3 days after disappearance of water on surface) in transplanted rice and application of wheat residues @ 5 t/ha in rice is an appropriate moisture conservation technique for improving the physiological growth and productivity, besides enhancing the soil fertility.
Effectiveness of Reminiscence Therapy on Self-health Perception and Self-esteem among Elderly Residing at Selected Old Age Homes
Authors: Rajan N. M. Reshma , Imran Syed 
Published: October 2021

Purpose: To assess the level of self-health perception and self-esteem among elderly residing at old age homes and to determine the effect of reminiscence therapy on the level of self-health perception and self-esteem.
Methods: Quasi-experimental pre-test post-test control group design was carried out on 80 elderly residing at old age homes selected by non-probability convenient sampling technique to test effectiveness of reminiscence therapy. The data was collected by using self-health perception scale consist 32 items and Rosenberg self-esteem scale consist 10 items.
Results: The result showed thatthere was a significant difference in self-esteem in the intervention group after the intervention (p=0.03<0.05) and there was no significant difference in self-esteem of the control group (p=0.83>0.05), whereas in self-health perception there was no significant difference in both intervention and control group (p=0.18 & 0.95>0.05). Between the groups there was no significant difference (p>0.05) found in the protest score of self-esteem and self-health perception between intervention and control group, while there is a significant difference found in post-test of self-esteem (p=0.002) and self-health perception score (p=0.004) between the groups. The obtained p values of chi-square and likelihood ratio test were >0.05. Only a few associations were found between both self-health perception and self-esteem with demographic variables that are sex and previous occupation/job in the control group.
Conclusion: Reminiscence therapy proved to be effective in improving self-esteem among elderly residing in selected old age homes at Mangaluru, whereas not shown effectiveness in improving self-health perception.