bluuebunny/crossref_metadata_2025_split huggingface dataset:
​Estimation of Yield and Yield-related Traits among Seedling Colnes of Mango [Mangifera indica (L.) Cv. Langra] using Correlation and Path Coefficient
Authors: Rajpoot Megha , Sharma T.R. , Sharma R.M. 
Published: October 2021

Background: Mango is one of the most important fruits in the world. The superior position of mango is due to its very wide and variety utilization and because of that, the main goal of all mango breeding programs is to obtain new inbred and hybrids that will outperform the existing hybrids concerning many traits for efficient selection of fruit yield, like the most important economic traits, regarding its on the great influence the environmental factors, has a complex mode of inheritance and low heritable is necessary to know the relation between fruit yield and other traits which are influencing on fruit yield. The current study aimed to verify the variability and correlation between the traits in fruit yield, to practice indirect selection. Methods: In this field investigation during 2018-19, Forty diverse clones (vegetative propagate) of mango cv. Langra were selected on Fruit Research Station (Farm), Imalia, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (M.P.) to determine the genotypic correlation along with their direct and indirect effects through path coefficient analysis in mango as to estimate the contribution of most important characters towards yield. Result: Yield was positively and significantly correlated with panicle length (0.38**), length of leaves (0.22*). The residual effect of path analysis was -0.06 revealed higher genetic variability and also proved a lower per cent of environmental influence on the selected characters of mango clones of Langra. Higher positive direct effects on yield were exhibited by the length of leaves, the width of leaves, flower shoot length, number of panicles, number of flowers, and fruit drop %. In combination with correlation coefficient and path analysis, it was found that the length of leaves gave a significant positive correlation coefficient with yield and also produce a high positive direct effect.
Efficient Anaerobic Consumption of D-xylose by E. coli BL21(DE3) via xylR Adaptive Mutation
Authors: Heo Jung Min , Kim Hyun Ju , Lee Sang Jun 
Published: October 2021

Abstract
Background: Microorganisms can prioritize the uptake of different sugars depending on their metabolic needs and preferences. When both D-glucose and D-xylose are present in growth media, E. coli cells typically consume D-glucose first and then D-xylose. Similarly, when E. coli BL21(DE3) is provided with both glucose and xylose under anaerobic conditions, glucose is consumed first, whereas xylose is consumed very slowly.Results: When BL21(DE3) was adaptively evolved via subculture, the consumption rate of D-xylose increased gradually. Strains JH001 and JH019, whose D-xylose consumption rate was faster, were isolated after subculture. Genome analysis of the JH001 and JH019 strains revealed that C91A (Q31K) and C740T (A247V) missense mutations in the xylR gene (which encodes the XylR transcriptional activator), respectively, controlled the expression of the xyl operon. RT-qPCR analyses demonstrated that the XylR mutation caused a 10.9-fold and 3.5-fold increase in the expression of the xylA (xylose isomerase) and xylF (xylose transporter) genes, respectively, in the adaptively evolved JH001 and JH019 strains. A C91A adaptive mutation was introduced into a new BL21(DE3) background via single-base genome editing, resulting in immediate and efficient D-xylose consumption. Conclusions: We obtained anaerobically-adapted BL21(DE3) cells through short-term adaptive evolution and identified xylR mutations responsible for faster xylose consumption, which may facilitate the improvement of microbial fermentation technology.
First measurement of decimeter-sized rocky material in the Oort cloud
Authors: Vida Denis , Brown Peter , Devillepoix Hadrien , Wiegert Paul , Moser Danielle , Matlovič Pavol , Herd Christopher , Hill Patrick , Tóth Juraj , Cooke William , Hladiuk Donald 
Published: October 2021

Abstract
The Oort cloud is thought to be a reservoir of icy planetesimals and a source of long-period comets (LPCs) implanted from the outer Solar System during the time of giant planet formation. The presence of rocky ice-free bodies is much harder to explain. The rocky fraction in the Oort cloud is a key diagnostic of Solar System formation models as this ratio can distinguish between "massive" and "depleted" proto-asteroid belt scenarios and thus disentangle competing planet formation models. Objects of asteroidal appearance have been telescopically observed on LPC orbits, but from reflectance spectra alone it is uncertain whether they are asteroids or extinct comets. Here we report a first direct observation of a decimeter-sized rocky meteoroid on a retrograde LPC orbit (e ≈ 1.0, i = 121°). The ~2 kg object entered the atmosphere at 62 km/s. The associated fireball terminated at 46.5 km, 40 km deeper than cometary objects of similar mass and speed. During its flight, it experienced dynamic pressures of several MPa, comparable to meteorite-dropping fireballs. In contrast, cometary material measured by Rosetta have compressive strengths of ~1 kPa. The earliest fragmentation of this fireball occurred at >100 kPa, indicating it had a minimum global strength well in excess of cometary. A numerical ablation model produces bulk density and ablation properties consistent with asteroidal meteoroids. We estimate the flux of rocky objects impacting Earth from the Oort cloud to be ~0.7 × 106 km2 per year to a mass limit of 10 g. This is ~6% of the total flux of fireballs on LPC-orbits to these masses. Our results suggests there is a high fraction of asteroidal material in the Oort cloud at small sizes and gives support to migration-based dynamical models of the formation of the Solar System which predict that significant rocky material is implanted in the Oort cloud, a result not explained by traditional Solar System formation models.
Heat shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition
Authors: Lu Shan , Hu Jiaojiao , Aladesuyi Bankhole , Goginashvili Alexander , Vazquez-Sanchez Sonia , Diedrich Jolene , Gu Jinge , Blum Jacob , Oung Spencer , Yu Haiyang , Ravits John , Liu Cong , Yates John , Cleveland Don 
Published: October 2021

Abstract
While the RNA binding protein TDP-43 reversibly phase separates within nuclei into complex droplets (anisosomes) with TDP-43-containing liquid outer shells and liquid centers of HSP70 family chaperones, cytoplasmic aggregates of TDP-43 are hallmarks of multiple neurodegenerative diseases, including ALS. Here we show that transient oxidative stress, proteasome inhibition, or inhibition of HSP70’s ATP-dependent chaperone activity provokes reversible cytoplasmic TDP-43 de-mixing and transition from liquid to gel/solid, independent of RNA binding or stress granules. Isotope labeling mass spectrometry is used to identify that phase separated cytoplasmic TDP-43 is primarily bound by the small heat shock protein HSPB1. Binding is direct, mediated through TDP-43’s RNA binding and low complexity domains. HSPB1 partitions into TDP-43 droplets, inhibits TDP-43 assembly into fibrils, and is essential for disassembly of stress-induced, TDP-43 droplets. Decrease of HSPB1 promotes cytoplasmic TDP-43 de-mixing and mislocalization. HSPB1 depletion is identified within ALS-patient spinal motor neurons containing aggregated TDP-43. These findings identify HSPB1 to be a regulator of cytoplasmic TDP-43 phase separation and aggregation.
Entrepreneurship and the Promises of Inclusive Urban Development in Ethiopia
Authors: Kebede Getahun Fenta 
Published: October 2021

Abstract
Ethiopia is one of the rapidly urbanizing countries in Africa and rural-urban migration is the major factor in the urbanization process. Migration is selective and rural youth are more likely to migrate to cities than others. However, the capacity of cities to accommodate the massive influx of migrants by providing formal employment is limited. Consequently, migrants remain marginalized and without access to employment opportunities. The majority are pushed into self-employment in the informal sector with few entrepreneurial skills and no access to affordable finance. Besides, though Ethiopia has shown economic growth, the challenges posed by a fast-growing young population has increased urban inequality, making the youth vulnerable. Despite such challenges, harnessing the benefits of the youth bulge and promoting inclusive development through the promotion of entrepreneurship has become a priority area since 1990s. Although improvements have been made, entrepreneurship programs are unable to reach the unemployed youth and those engaged in informal sector. The objective of this paper is to explore barriers that hinder the youth to formalize informal businesses and to start new businesses. The study followed a qualitative approach. Data were collected through key informant interviews and focus group discussions from four cities-Addis Ababa, Adama, Bahir Dar and Hawassa. The findings show that several bottlenecks including politicization of entrepreneurship, lack of understanding of the nature and demands of the youth; weak instructional systems; low levels of service capacity and inefficiency; lack of entrepreneurship education, youth negligence, and corruption hinder entrepreneurship programs and thereby attaining inclusive development in Ethiopia.
Sustainment of Diverse Evidence-Based Practices Disseminated in the Veterans Health Administration (VHA): Development and Administration of a Pragmatic Instrument
Authors: Reardon Caitlin , Nevedal Andrea , Widerquist Marilla A. Opra , Arasim Maria , Jackson George L. , White Brandolyn , Cutrona Sarah L. , Fix Gemmae M. , Gifford Allen L. , DeLaughter Kathryn , King Heather A. , Henderson Blake , Vega Ryan , Damschroder Laura 
Published: October 2021

Abstract
Background There are challenges associated with measuring sustainment of evidence-based practices (EBPs). First, the terms sustainability and sustainment are often falsely conflated: sustainability assesses the likelihood of an EBP being in use in the future while sustainment assesses the extent to which an EBP is (or is not) in use. Second, grant funding often ends before sustainment can be assessed. The Veterans Health Administration (VHA) Diffusion of Excellence (DoE) is one of few large-scale models of diffusion; it seeks to identify and disseminate practices across the VHA system. The DoE sponsors “Shark Tank” competitions, in which leaders bid on the opportunity to implement a practice with 6 months of implementation support. As part of an ongoing evaluation of the DoE, we sought to develop and administer a pragmatic instrument to assess sustainment of DoE practices.Methods In June 2020, surveys were sent to 64 facilities that were part of the DoE evaluation. We began analysis by comparing alignment of quantitative and qualitative responses; some facility representatives reported in the open text box of the survey that their practice was on a temporary hold due to COVID-19 but answered the primary outcome question differently. As a result, the team reclassified the primary outcome of these facilities to Sustained: Temporary COVID-Hold. Following this reclassification, the number and percent of facilities in each category was calculated. We used directed content analysis, guided by the Consolidated Framework for Implementation Research (CFIR), to analyze open text box responses. Results A representative from forty-one facilities (64%) completed the survey. Among responding facilities, 29/41 sustained their practice, 1/41 partially sustained their practice, 8/41 had not sustained their practice, and 3/41 had never implemented their practice. Sustainment rates increased between Cohorts 1 – 4. Conclusions The development and administration of our pragmatic survey allowed us to assess sustainment of DoE practices. Planned updates to the survey will enable flexibility in assessing sustainment and its determinants at any phase after adoption. This assessment approach can flex with the longitudinal and dynamic nature of sustainment, including capturing nuances in outcomes when practices are on a temporary hold.
Loss of Aly/ALYREF Suppresses Toxicity in Both Tau and TDP-43 Models of Neurodegeneration
Authors: Kow Rebecca , Black Aristide , Saxton Aleen , Liachko Nicole , Kraemer Brian 
Published: October 2021

Abstract
Background Neurodegenerative diseases with tau pathology, or tauopathies, include Alzheimer’s Disease and related dementia disorders. Previous work has shown that loss of the poly(A) RNA-binding protein gene sut2/MSUT2 strongly suppressed tauopathy in C. elegans, human cell culture, and mouse models of tauopathy. However, the mechanism of suppression is still unclear. Recent work has shown that MSUT2 protein interacts with the THO complex and ALYREF, which are components of the mRNA nuclear export complex. Additionally, previous work showed ALYREF homolog Ref1 modulates TDP-43 and G 4 C 2 toxicity in D. melanogaster models. Methods We used transgenic C. elegans models of tau or TDP-43 toxicity to investigate the effects of loss of ALYREF function on tau and TDP-43 toxicity. In C. elegans, there are three genes that are homologous to human ALYREF: aly-1, aly-2, and aly-3. Results We found that loss of C. elegans aly gene function, especially loss of both aly-2 and aly-3, suppressed tau-induced toxic phenotypes. Loss of aly-2 and aly-3 was also able to suppress TDP-43-induced behavioral deficits. However, loss of aly-2 and aly-3 had divergent effects on mRNA and protein levels as total tau protein levels were reduced while mRNA levels were increased, but no significant effects were seen on total TDP-43 protein or mRNA levels. Conclusions Our results suggest that although aly genes modulate both tau and TDP-43-induced toxicity phenotypes, the molecular mechanisms of suppression are different and separated from impacts on mRNA and protein levels. Altogether this study highlights the importance of elucidating RNA-related mechanisms in both tau and TDP-43-induced toxicity.
Downregulation of NEDD4L by EGFR Signaling Promotes the Development of Lung Adenocarcinoma
Authors: Li Guoyin , Song Zewen , Wu Changjing , Li XiaoYan , Zhao Liping , Tong Binghua , Guo Zhenni , Sun Meiqing , Zhao Jin , Zhang Huina , Jia Lintao , Li Shengqing , Wang Lei 
Published: October 2021

Abstract
Cumulative evidence indicates that the abnormal regulation of the NEDD4 family of E3-ubiquitin ligases participates in the tumorigenesis and development of cancer. However, their role in lung adenocarcinoma (LUAD) remains unclear. This study comprehensively analyzed the NEDD4 family in LUAD data sets from public databases and found only NEDD4L was associated with the overall survival of LUAD patients. Gene set enrichment analysis (GSEA) indicated that NEDD4L might be involved in the regulation of mTORC1 pathway. Both cytological and clinical assays showed that NEDD4L inhibited the activity of the mTOR signaling pathway. In vivo and in vitro experiments showed that NEDD4L could significantly inhibit the proliferation of LUAD cells. In addition, this study also found that the expression of NEDD4L was regulated by EGFR signaling. These findings firstly revealed that NEDD4L mediates an interplay between EGFR and mTOR pathways in LUAD, and suggest that NEDD4L held great potential as a novel biomarker and therapeutic target for LUAD.
Antimicrobial Resistance (AMR) in COVID-19 Patients: A Systematic Review and Meta-Analysis (November 2019 - June 2021)
Authors: Kariyawasam Ruwandi M. , Julien Danielle A. , Jelinski Dana C. , Larose Samantha L. , Rennert-May Elissa , Conly John M. , Dingle Tanis C. , Chen Justin Z. , Tyrrell Gregory J. , Ronksley Paul E. , Barkema Herman W. 
Published: October 2021

Abstract
BackgroundPneumonia from SARS-CoV-2 is difficult to distinguish from other viral and bacterial etiologies. Broad-spectrum antimicrobials are frequently prescribed to patients hospitalized with COVID-19 which potentially acts as a catalyst for the development of antimicrobial resistance (AMR). ObjectivesWe conducted a systematic review and meta-analysis during the first 18 months of the pandemic to quantify the prevalence and types of resistant co-infecting organisms in patients with COVID-19 and explore differences across hospital and geographic settings.MethodsWe searched MEDLINE, Embase, Web of Science (BioSIS), and Scopus from November 1, 2019 to May 28, 2021 to identify relevant articles pertaining to resistant co-infections in patients with laboratory confirmed SARS-CoV-2. Patient- and study-level analyses were conducted. We calculated pooled prevalence estimates of co-infection with resistant bacterial or fungal organisms using random effects models. Stratified meta-analysis by hospital and geographic setting was also performed to elucidate any differences. ResultsOf 1331 articles identified, 38 met inclusion criteria. A total of 1959 unique isolates were identified with 29% (569) resistant organisms identified. Co-infection with resistant bacterial or fungal organisms ranged from 0.2 to 100% among included studies. Pooled prevalence of co-infection with resistant bacterial and fungal organisms was 24% (95% CI: 8-40%; n=25 studies: I2=99%) and 0.3% (95% CI: 0.1-0.6%; n=8 studies: I2=78%), respectively. Among multi-drug resistant organisms, methicillin-resistant Staphylococcus aureus, carbapenem-resistant Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida auris were most commonly reported. Stratified analyses found higher proportions of AMR outside of Europe and in ICU settings, though these results were not statistically significant. Patient-level analysis demonstrated >50% (n=58) mortality, whereby all but 6 patients were infected with a resistant organism. ConclusionsDuring the first 18 months of the pandemic, AMR was moderately prevalent in COVID-19 patients and varied by hospital and geography although there was substantial heterogeneity. Given the variation in patient populations within these studies, clinical settings, practice patterns, and definitions of AMR, further research is warranted to quantify AMR in COVID-19 patients to improve surveillance programs, infection prevention and control practices and antimicrobial stewardship programs globally.
Increase in Tibial Internal Rotation Due to Weight-bearing is a Key Feature to Diagnose Early-stage Knee Osteoarthritis: A Study with Upright Computed Tomography
Authors: Kaneda Kazuya , Harato Kengo , Oki Satoshi , Yamada Yoshitake , Nakamura Masaya , Nagura Takeo , Jinzaki Masahiro 
Published: October 2021

Abstract
Background The classification of knee osteoarthritis is an essential clinical issue, particularly in terms of diagnosing early knee osteoarthritis. However, the evaluation of three-dimensional limb alignment on two-dimensional radiographs is limited. This study evaluated the three-dimensional changes induced by weight-bearing in the alignments of lower limbs at various stages of knee osteoarthritis.Methods 45 knees of 25 patients (69.9 ± 8.9 years) with knee OA were examined in the study. CT images of the entire leg were obtained in the supine and standing positions using conventional CT and 320 low-detector upright CT, respectively. Next, the differences in the three-dimensional alignment of the entire leg in the supine and standing positions were obtained using 3D-3D surface registration technique, and those were compared for each Kellgren–Lawrence grade. Results Increased flexion, adduction, and tibial internal rotation were observed in the standing position, as opposed to the supine position. Kellgren–Lawrence grades 1 and 4 showed significant differences in flexion, adduction, and tibial internal rotation between two postures. Grades 2 and 4 showed significant differences in adduction, while grades 1 and 2, and 1 and 3 showed significant differences in tibial internal rotation between standing and supine positions.Conclusions Weight-bearing increased the three-dimensional deformities in knees with osteoarthritis. Particularly, increased tibial internal rotation was observed in patients with grades 2 and 3 compared to those with grade 1. The increase in tibial internal rotation due to weight-bearing is a key pathologic feature to detect early osteoarthritic change in knees undergoing osteoarthritis.
Symptoms are the Most Effective Child SCAT5 Component for Recognizing Concussion on the Day of Injury
Authors: Erdman Nicholas Kevin , Kelshaw Patricia M. , Hacherl Samantha L. , Caswell Shane V. 
Published: October 2021

Abstract
Background: The Child Sport Concussion Assessment Tool 5th Edition (Child SCAT5) was developed to evaluate children between 5-12 years of age for a suspected concussion. However, limited empirical evidence exists demonstrating the value of the Child SCAT5 for acute concussion assessment. Therefore, the purpose of our study was to examine differences and assess the diagnostic properties of Child SCAT5 scores among concussed and non-concussed middle school children on the same day as a suspected concussion.Methods: Our participants included 34 concussed (21 boys, 13 girls; age=12.8±0.86 years) and 44 non-concussed (31 boys, 13 girls; age=12.4±0.76 years) middle school children who were administered the Child SCAT5 upon suspicion of a concussion. Child SCAT5 scores were calculated from the symptom evaluation (total symptoms, total severity), child version of the Standardized Assessment of Concussion (SAC-C), and modified Balance Error Scoring System (mBESS). The Child SCAT5 scores were compared between the concussed and non-concussed groups. Non-parametric effect sizes (r=z/√n) were calculated to assess the magnitude of difference for each comparison. The diagnostic properties (sensitivity, specificity, diagnostic accuracy, predictive values, likelihood ratios, and diagnostic odds ratio) of each Child SCAT5 score were also calculated.Results: Concussed children endorsed more symptoms (p<0.001, r=0.45), higher symptom severity (p<0.001, r=0.44), and had higher double leg (p=0.046, r=0.23), single leg (p=0.035, r=0.24), and total scores (p=0.022, r=0.26) for the mBESS than non-concussed children. No significant differences were observed for the SAC-C scores (p’s≥0.542). The quantity and severity of endorsed symptoms had the best diagnostic accuracy (AUC=0.76–0.77), negative predictive values (NPV=0.84–0.88), and negative likelihood ratios (-LR=0.22–0.31) of the Child SCAT5 scores.Conclusions: The symptom evaluation was the most effective component of the Child SCAT5 for differentiating between concussed and non-concussed middle school children on the same day as a suspected concussion.