bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of technical failures on recombinant production of soluble proteins in Escherichia coli: a case study on process and protein robustness
Authors: Pekarsky Alexander , Reininger Melanie , Spadiut Oliver 
Published: January 2021

AbstractTechnical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently, we published a study on the impact of technical failures on an inclusion body process that showed high robustness due to the inherent stability of IBs. In this follow-up study, we investigated the influence of technical failures during production of two soluble, cytosolic proteins in E. coli BL21(DE3). Cell physiology, productivity and protein quality were analyzed, after technical failures in aeration, substrate supply, temperature and pH control had been triggered. In most cases, cell physiology and productivity recovered during a subsequent regeneration phase. However, our results highlight that some technical failures lead to persistent deviations and affect the quality of purified protein.
Orlicz–Minkowski flows
Authors: Bryan Paul , Ivaki Mohammad N. , Scheuer Julian 
Published: January 2021

AbstractWe study the long-time existence and behavior for a class of anisotropic non-homogeneous Gauss curvature flows whose stationary solutions, if they exist, solve the regular Orlicz–Minkowski problems. As an application, we obtain old and new existence results for the regular even Orlicz–Minkowski problems; the corresponding$$L_p$$Lpversion is the even$$L_p$$Lp-Minkowski problem for$$p>-n-1$$p>-n-1. Moreover, employing a parabolic approximation method, we give new proofs of some of the existence results for thegeneralOrlicz–Minkowski problems; the$$L_p$$Lpversions are the even$$L_p$$Lp-Minkowski problem for$$p>0$$p>0and the$$L_p$$Lp-Minkowski problem for$$p>1$$p>1. In the final section, we use a curvature flow with no global term to solve a class of$$L_p$$Lp-Christoffel–Minkowski type problems.
An existence theorem for Brakke flow with fixed boundary conditions
Authors: Stuvard Salvatore , Tonegawa Yoshihiro 
Published: January 2021

AbstractConsider an arbitrary closed, countably n-rectifiable set in a strictly convex $$(n+1)$$

(
n
+
1
)

-dimensional domain, and suppose that the set has finite n-dimensional Hausdorff measure and the complement is not connected. Starting from this given set, we show that there exists a non-trivial Brakke flow with fixed boundary data for all times. As $$t \uparrow \infty $$

t
↑
∞

, the flow sequentially converges to non-trivial solutions of Plateau’s problem in the setting of stationary varifolds.
Temperature measurement with thin film sensors during warm forging of steel
Authors: Plogmeyer M. , Kruse J. , Stonis M. , Paetsch N. , Behrens B.-A. , Bräuer G. 
Published: January 2021

AbstractWarm forged components have better surface properties and higher dimensional accuracy than hot forged components. Diamond-like-carbon (DLC) coatings can be used as wear protection coatings, which are anti-adhesive and extremely hard (up to 3500 HV), to increase tool service life. In the first funding period of the research project at the IPH – Institut für Integrierte Produktion Hannover gGmbH and the Institute for Surface Technology (IOT) of the Technical University of Braunschweig in cooperation with the Fraunhofer Institute for Surface Engineering and Thin Films (IST), the influence of different coating types and process temperatures on tool wear was investigated. The result is, that DLC coatings can reduce tool wear in some cases significantly, but that their service life is strongly dependent on the temperature. Coating-integrated temperature measurement could not be realised at that point, due to adhesion challenges. During the second funding period, the effect of multilayer DLC coatings on tool wear was investigated. Also, an additional method of the temperature measurement on the engraving surface using thin film sensors was developed in order to correlate the local process temperature and local layer wear. In this work, the development of and the results gathered by the thin film temperature sensors are presented, which enable for more accurate temperature measurements than commonly used thermocouples. Their functionality and durability under high loads were investigated and showed to be promising.
Contemporary functional neuroanatomy and pathophysiology of dystonia
Authors: Brüggemann Norbert 
Published: January 2021

AbstractDystonia is a disabling movement disorder characterized by abnormal postures or patterned and repetitive movements due to co-contraction of muscles in proximity to muscles desired for a certain movement. Important and well-established pathophysiological concepts are the impairment of sensorimotor integration, a loss of inhibitory control on several levels of the central nervous system and changes in synaptic plasticity. These mechanisms collectively contribute to an impairment of the gating function of the basal ganglia which results in an insufficient suppression of noisy activity and an excessive activation of cortical areas. In addition to this traditional view, a plethora of animal, genetic, imaging and electrophysiological studies highlight the role of the (1) cerebellum, (2) the cerebello-thalamic connection and (3) the functional interplay between basal ganglia and the cerebellum in the pathophysiology of dystonia. Another emerging topic is the better understanding of the microarchitecture of the striatum and its implications for dystonia. The striosomes are of particular interest as they likely control the dopamine release via inhibitory striato-nigral projections. Striosomal dysfunction has been implicated in hyperkinetic movement disorders including dystonia. This review will provide a comprehensive overview about the current understanding of the functional neuroanatomy and pathophysiology of dystonia and aims to move the traditional view of a ‘basal ganglia disorder’ to a network perspective with a dynamic interplay between cortex, basal ganglia, thalamus, brainstem and cerebellum.
Numerical model of energy dissipation and shallow-water sloshing motions in tank under different coupled excitations
Authors: Su Yan 
Published: January 2021

AbstractShallow-water sloshing motions in a three-dimensional rectangular tank are investigated. The Boussinesq-type equations in terms of velocity potential and the finite-difference scheme are applied for the solutions of numerical model. Through linking the rate of decay of the wave amplitudes to the energy dissipation due to the friction at the tank walls, a linear damping term is proposed and added into the free surface boundary condition. Taking the tank under excited frequencies near the lowest natural frequency, the maximum transient wave amplitudes and steady-state wave amplitudes of sloshing motions at the tank wall are presented and verified by the experimental results given in the literature. The characteristics of sloshing motions in tank under different coupled excitations are studied. The results indicate that coupled surge-sway excitations lead to the weaker nonlinear sloshing motions in tank than the single degree of freedom excitations. The intersection of sloshing wave crest lines finally tend to the diagonal line of the tank under the coupled surge-sway excitations with different amplitudes. And the irregular free surface oscillations appear at the corners of the tank excited by the coupled surge-sway-roll-pitch-yaw harmonic motions.
Fulminant myocarditis parvovirus B19 related in a young woman
Authors: Pesce Rita , Taffarello PierPaolo , Rizzo Stefania , Basso Cristina , Cacciavillani Luisa , Del Monte Alvise , Babuin Luciano , Gerosa Gino , Bottio Tomaso 
Published: January 2021

AbstractWe present the case of a 18-year-old female with fulminant lymphocytic myocarditis caused by Parvovirus B19 (PVB19), successfully treated using temporary LVAD. In the literature there is no consensus on the surgical strategy. While some surgeons prefer to use a single device supporting only the LV, others prefer to start immediately with a biventricular supporting. At pre-procedural ultrasound evaluation, her anatomical features were not suitable for a percutaneous device such as the Impella. Thus, a temporary paracorporeal continuous flow LVAD was inserted. The heart recovery allowed LVAD removal 9 days after the implant.
Adultism at the Root of Youth Maltreatment in A. S. King’s Still Life with Tornado
Authors: Dziri Nourhene 
Published: January 2021

AbstractWhile child abuse and neglect have been explored at length, less attention has been paid to the role of contemporary realist Young Adult Literature (YAL) in denouncing abusive treatments of young people in conjunction with adultism. It is hereby suggested that age inequality is at the core of youth maltreatment, and it is this intersectionality that should lead attempts to confront this global problem. YAL offers a bottom-up approach to deal with these social issues through first-hand experiences of oppressed, yet cognizant protagonists. This article foregrounds the voice of Sarah, the lead character featured in A. S. King’s Still Life with Tornado (2016), in addressing the drastic outcomes of ageist stereotypes at the family level. The emphasis on the perspective of young people provides an informative understanding of youth subordination, parental power, and adultist discrimination in an effort to challenge such practices.
Climate change—that is not real! A comparative analysis of climate-sceptic think tanks in the USA and Germany
Authors: Busch Timo , Judick Lena 
Published: January 2021

AbstractThe science is clear: climate change is real. In 2015, 195 countries adopted the global climate deal in Paris. Nonetheless, numerous well-organized conservative think tanks (CTTs) deny that climate change is happening. We ask what kind of counterclaims are used by climate-sceptic CTTs and to what extent these counterclaims change over time. We analyse about 2500 blog articles from prominent CTTs in the USA and Germany between 2008 and 2016. Our results show that sceptical arguments about climate policy and science dominate the countermovement. At the same time, we detect that the prevalence of counterclaims is CTT-specific and that US think tanks show a greater variability compared to their German counterparts. In a surprising outcome, we find that the Paris Agreement did not affect the climate denial movement. Based on these insights, we discuss our contributions to social movement research in the climate change denial context and derive conclusions for pro-climate campaigns.
Reviewing qualitative research approaches in the context of critical infrastructure resilience
Authors: Cantelmi R. , Di Gravio G. , Patriarca R. 
Published: January 2021

AbstractModern societies are increasingly dependent on the proper functioning of critical infrastructures (CIs). CIs produce and distribute essential goods or services, as for power transmission systems, water treatment and distribution infrastructures, transportation systems, communication networks, nuclear power plants, and information technologies. Being resilient becomes a key property for CIs, which are constantly exposed to threats that can undermine safety, security, and business continuity. Nowadays, a variety of approaches exist in the context of CIs’ resilience research. This paper provides a state-of-the-art review on the approaches that have a complete qualitative dimension, or that can be used as entry points for semi-quantitative analyses. The study aims to uncover the usage of qualitative research methods through a systematic review based on PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). The paper identifies four principal dimensions of resilience referred to CIs (i.e., techno-centric, organisational, community, and urban) and discusses the related qualitative methods. Besides many studies being focused on energy and transportation systems, the literature review allows to observe that interviews and questionnaires are most frequently used to gather qualitative data, besides a high percentage of mixed-method research. The article aims to provide a synthesis of literature on qualitative methods used for resilience research in the domain of CIs, detailing lessons learned from such approaches to shed lights on best practices and identify possible future research directions.
Is a Negative Attentional Bias in Individuals with Autism Spectrum Disorder Explained by Comorbid Depression? An Eye-Tracking Study
Authors: Bergman M. Annemiek , Vrijsen Janna N. , Rinck Mike , van Oostrom Iris , Kan Cornelis C. , Collard Rose M. , van Eijndhoven Philip , Vissers Constance Th. W. M. , Schene Aart H. 
Published: January 2021

AbstractHeightened attention towards negative information is characteristic of depression. Evidence is emerging for a negative attentional bias in Autism spectrum disorder (ASD), perhaps driven by the high comorbidity between ASD and depression. We investigated whether ASD is characterised by a negative attentional bias and whether this can be explained by comorbid (sub) clinical depression. Participants (n = 116) with current (CD) or remitted depression (RD) and/or ASD, and 64 controls viewed positively and negatively valenced (non-)social pictures. Groups were compared on three components of visual attention using linear mixed models. Both CD individuals with and without ASD, but not remitted depressed and never-depressed ASD individuals showed a negative bias, suggesting that negative attentional bias might be a depressive state-specific marker for depression in ASD.
Signaling pathways in human osteoclasts differentiation: ERK1/2 as a key player
Authors: Pennanen Paula , Kallionpää Roope A. , Peltonen Sirkku , Nissinen Liisa , Kähäri Veli-Matti , Heervä Eetu , Peltonen Juha 
Published: January 2021

AbstractLittle is known about the signaling pathways involved in the differentiation of human osteoclasts. The present study evaluated the roles of the Ras/PI3K/Akt/mTOR, Ras/Raf/MEK1/2/ERK1/2, calcium-PKC, and p38 signaling pathways in human osteoclast differentiation. Mononuclear cells were isolated from the peripheral blood of control persons and patients with neurofibromatosis 1 (NF1), and the cells were differentiated into osteoclasts in the presence of signaling pathway inhibitors. Osteoclast differentiation was assessed using tartrate-resistant acid phosphatase 5B. Inhibition of most signaling pathways with chemical inhibitors decreased the number of human osteoclasts and disrupted F-actin ring formation, while the inhibition of p38 resulted in an increased number of osteoclasts, which is a finding contradictory to previous murine studies. However, the p38 inhibition did not increase the bone resorption capacity of the cells. Ras-inhibitor FTS increased osteoclastogenesis in samples from control persons, but an inhibitory effect was observed in NF1 samples. Inhibition of MEK, PI3K, and mTOR reduced markedly the number of NF1-deficient osteoclasts, but no effect was observed in control samples. Western blot analyses showed that the changes in the phosphorylation of ERK1/2 correlated with the number of osteoclasts. Our results highlight the fact that osteoclastogenesis is regulated by multiple interacting signaling pathways and emphasize that murine and human findings related to osteoclastogenesis are not necessarily equivalent.
DNA-binding properties of the MADS-domain transcription factor SEPALLATA3 and mutant variants characterized by SELEX-seq
Authors: Käppel Sandra , Eggeling Ralf , Rümpler Florian , Groth Marco , Melzer Rainer , Theißen Günter 
Published: January 2021

Abstract

Key message

We studied the DNA-binding profile of the MADS-domain transcription factor SEPALLATA3 and mutant variants by SELEX-seq. DNA-binding characteristics of SEPALLATA3 mutant proteins lead us to propose a novel DNA-binding mode.

Abstract
MIKC-type MADS-domain proteins, which function as essential transcription factors in plant development, bind as dimers to a 10-base-pair AT-rich motif termed CArG-box. However, this consensus motif cannot fully explain how the abundant family members in flowering plants can bind different target genes in specific ways. The aim of this study was to better understand the DNA-binding specificity of MADS-domain transcription factors. Also, we wanted to understand the role of a highly conserved arginine residue for binding specificity of the MADS-domain transcription factor family. Here, we studied the DNA-binding profile of the floral homeotic MADS-domain protein SEPALLATA3 by performing SELEX followed by high-throughput sequencing (SELEX-seq). We found a diverse set of bound sequences and could estimate the in vitro binding affinities of SEPALLATA3 to a huge number of different sequences. We found evidence for the preference of AT-rich motifs as flanking sequences. Whereas different CArG-boxes can act as SEPALLATA3 binding sites, our findings suggest that the preferred flanking motifs are almost always the same and thus mostly independent of the identity of the central CArG-box motif. Analysis of SEPALLATA3 proteins with a single amino acid substitution at position 3 of the DNA-binding MADS-domain further revealed that the conserved arginine residue, which has been shown to be involved in a shape readout mechanism, is especially important for the recognition of nucleotides at positions 3 and 8 of the CArG-box motif. This leads us to propose a novel DNA-binding mode for SEPALLATA3, which is different from that of other MADS-domain proteins known.
Language skills, and not executive functions, predict the development of reading comprehension of early readers: evidence from an orthographically transparent language
Authors: Dolean Dacian Dorin , Lervåg Arne , Visu-Petra Laura , Melby-Lervåg Monica 
Published: January 2021

AbstractThe simple view of reading proposes that the development of reading comprehension in early elementary school is best predicted by children’s fluent decoding and oral language skills. Recent studies challenge this view and suggest that executive functions should also be included in this theoretical model; however, the empirical evidence is not strong enough to clearly support or refute this hypothesis. In this short-term longitudinal study, we used latent variables to test whether executive functions have direct effects on the development of reading comprehension in 184 Romanian second graders, beyond fluent decoding and oral language skills. The results indicated that the initial stages of reading comprehension were associated with executive functions, but only the language skills could independently predict the development of reading comprehension. Our findings show that executive functions do not have a significant direct effect on the development of reading comprehension in early readers beyond fluent decoding and oral language skills in languages with transparent orthography. The results also suggest that once children learn to decode well, their language skills (and not their executive functions) have a strong effect on the development of reading comprehension. Therefore, reading interventions in elementary school should stress on the development of oral language skills.
Judicial Response to the 2010 Horizontal Merger Guidelines
Authors: Shapiro Carl , Shelanski Howard 
Published: January 2021

AbstractWe study how the courts have responded to the 2010 Horizontal Merger Guidelines issued by the U.S. Department of Justice and the Federal Trade Commission. Looking at decided cases, we find that both the government and merging parties rely on the 2010 Guidelines in presenting their cases, each side respectively arguing that it should win if the court properly follows them
. The 2010 Guidelines had the strongest effect on the case law in the area of unilateral effects, where a number of courts have embraced them in ways that clearly depart from earlier decisions. The case law now exhibits much greater receptivity to a government showing that the merger will lead to higher prices simply due to the loss of direct competition between the two merging firms. The courts also have followed the 2010 Guidelines by more willingly defining markets around targeted customers. We do not detect any effect on decided cases of the higher concentration thresholds found in the 2010 Guidelines. Both the average pre-merger level of market concentration and the average increase in market concentration alleged by the government in litigated cases to date declined after 2010
.
Education as a Positional Good? Evidence from the German Socio-Economic Panel
Authors: Durst Alessa K. 
Published: January 2021

AbstractPeople care about their relative standing in society and therefore compare themselves to relevant others. Empirical findings suggest that there are concerns for relative standing for different goods and life domains such as income, cars, attractiveness, and supervisor’s praise. Even education has been mentioned as having a (partially) positional character. However, there has been only small consideration of education as a positional good in the empirical literature so far. Based on the literature on positional concerns and the role of education on relative position, I use German panel data to investigate the relationship between education and life satisfaction beyond the effect education might have through other variables such as income, health, or occupational prestige. Additionally, I consider the possibility that the consumption of education is subject to positional concerns. I discover a positive relationship between education and life satisfaction, indicating that education has a consumption component. Moreover, the relationship depends on the distribution of particular levels of education, suggesting that education has a positional character.
Seasonalities in the German stock market
Authors: Hofmann Daniel , Keiber Karl Ludwig 
Published: January 2021

AbstractThis paper suggests innovative investment strategies drawing on return seasonalities. By means of an out-of-sample study of the German stock market, we report that these long–short investment strategies earn on average raw returns up to 233 basis points per month throughout two decades from 1998 to 2017. On a monthly basis, this documents an outperformance of the corresponding Heston and Sadka (J Financ Econ 87(2):418–445, 2008) strategy by 66%. This outperformance is robust in magnitude even after adjusting for common risk factors along both the three-factor Fama and French (J Financ Econ 33(1):3–56, 1993) model and the four-factor Carhart (J Finance 52(1):57–82, 1997) model. Categorizing stocks into three risk profiles lets us conclude that long–short momentum portfolios of stocks with a low-risk profile generate robust investment performance.