bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Nrf2 Signaling in Development of Hepatocyte-Like Cells
Authors: Takasu Chie , Gao Luping , Saito Yu , Morine Yuji , Ikemoto Tetsuya , Yamada Shinichiro , Miyazaki Katsuki , Okikawa Shouhei , Shimada Mitsuo 
Published: August 2021

Abstract
Generation of hepatocytes from human adipose-derived mesenchymal stem cells (hADSCs) could be a promising alternative source of human hepatocytes. However, mechanisms to differentiate hepatocytes from hADSCs are not fully elucidated. In this study, we investigated the role of nuclear factor erythroid-2 related factor 2 (Nrf2) in differentiation of hepatocyte-like cells (HLCs). We used our established three-step differentiation protocol to develop HLCs from hADSCs. Significant nuclear translocation of Nrf2 occurred from day 11 (Step 2) until the end of HLC differentiation. There were no significant differences in Nrf2 translocation rates among the four experimental groups (activin-A, GSK3 inhibitor, Nrf2 siRNA, and control) at day 6 (end of Step 1). Nuclear translocation of Nrf2 in the GSK3 inhibitor-treated group was obviously higher than the other groups at day 11 (Step 2). Moreover, nuclear translocation of Nrf2 in the GSK3 inhibitor-treated group was notably higher than the other groups during Step 3. CYP3A4 activity (Luciferin-IPA assay) of the GSK3 inhibitor-treated group was significantly higher than the other three groups. Nrf2 was activated during differentiation of HLCs, and inhibition of Nrf2 delayed maturation and impaired the function of HLCs. Thus, Nrf2 might be a notable target for developing highly functional human HLCs.
Investigation of Crystal Structure, Electrical and Magnetic Properties of Spinel Mn-Cd Ferrite Nanoparticles
Authors: Badr M. H. , Nasr M. H. , Elkholy M. M. , El-Deen L. M. S. , Turky Gamal M. , Moustafa M. , Abouhaswa A. S. , Hamalawy A. A. EL 
Published: August 2021

Abstract
Fe2Cd1-xMnxO4 (x= 0, 0.25, 0.5, 0.75, 1) spinel ferrite nanoparticle samples were synthesized using a flash auto-combustion technique and analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). The inspection of dielectric and magnetic properties of the prepared ferrites was carried out by using broadband dielectric spectroscopy (BDS) and vibration sample magnetometer (VSM) measurements, respectively. X-ray diffraction analysis verified the formation of the main cubic phase with space group Fd3m and a decrease in lattice constants with the increase in Mn-content. FTIR study revealed the two characteristic absorption bands of spinel ferrites and their dependence on Mn-content was investigated and explained. Elastic moduli such as Longitudinal, transverse and mean elastic wave velocities (𝑉𝑙 , 𝑉𝑡 𝑉𝑚), bulk modulus (𝐵), rigidity modulus (𝐺), Young modulus (𝐸) and calculated Debye temperature (θD) have been studied and found to decrease with the increase in Mn-content. SEM analysis revealed nanoparticles agglomeration of uniform grains with increasing in the average grain size as Mn-content increased. The VSM measurements showed an increase in saturation magnetization (Ms) accompanied by a decrease in coercivity (Hc) as Mn-content increased. Dielectric investigations showed very high values of permittivity and dielectric loss at lower frequencies reflecting the combination of exchange of electrons between ferrous and ferric ions and ions’ transport. The ac-conductivity showed a plateau that yields the dc-conductivity (σdc) at lower frequencies followed by a characteristic frequency (νc) at which it tends out to follow a power law. The relation between these two parameters confirms the empirical BNN-relation.
Pannexin-1 promotes the invasion of pituitary adenoma by modulating ATP release
Authors: Tang Xiaoshuang 
Published: August 2021

Abstract
Background

Pannexin-1 (Panx1) channel participates in the development of many tumor subtypes, however, its role of Panx1 in pituitary adenoma (PA) remains largely unknown. The current study was designed to investigate the role of Panx1 in PA, especially in the invasion of PA.
Methods

We examined the expression of Panx1 in 56 surgical PA samples, which were divided into invasive and noninvasive groups. We also established Panx1-overexpressing (Panx1-OE) PA cells using the GH3 and MMQ cell lines in vitro.
Results

We found that the expression of Panx1 in invasive PA was significantly upregulated compared with noninvasive PA and pituitary gland and that Panx1-OE could promote the invasion of these cell lines but had no effect on their proliferation. Further metabolomics experiments confirmed that Panx1-OE could trigger changes in several metabolic pathways of GH3 cells. Among the dysregulated cellular metabolites, we found that adenosine triphosphate (ATP) could be released to the extracellular matrix through the Panx1 channel and thereby enhance the invasion of GH3 cells. Mechanistically, ATP promoted extracellular Ca2+ influx and upregulated the expression of secretagogin and MMP-2/9 by activating the P2X7 receptor (P2X7R). We also found that the Panx1-ATP-P2X7R signaling pathway may promote GH3 cell invasion by remodeling the actin cytoskeleton.
Conclusion

Taken together, our findings point to a pivotal role of Panx1 in promoting PA invasion, indicating its use as a potential therapeutic target for invasive PA.
Rheumatoid arthritis of menopause: there are more questions than answers
Authors: Khashukoeva A. Z. , Naumov A. V. , Meshkov A. D. , Rassokhina O. V. 
Published: August 2021

The article is devoted to an urgent issue - rheumatoid arthritis (RA). The symptoms of arthritis appear in women during menopause. Literature data show that it is often during period of poor reproductive function that the disease first manifests itself in women. RA significantly affects the prognosis of women’s life expectancy and quality. The clinical and pathogenetic relationships between autoimmune diseases (one of which is RA) and the features of menopause remain poorly understood and require research. Basically, papers devoted to the problem present epidemiological studies and the study of the relationships between sex hormones levels and inflammatory mediators that are specific to RA.A literature review on the features of the onset and course of rheumatoid arthritis in menopausal women is presented. The most accepted in the literature explanations characterizing the relationship between changes during menopause and RA are arguments about the protective effect of estrogens on the cells of the musculoskeletal system and the ability of estrogens to influence the intensity of inflammation (systemic inflammation of low intensity associated with age). In the case of the development of rheumatoid arthritis in old age, higher levels of IL-6 and lower TNFa are revealed. This fact gives rise to the choice of basic therapy in favor of a humanized monoclonal antibody to the human interleukin-6 receptor - tocilizumab.The review may be of interest to both practising rheumatologists, neurologists, and gynecologists and general practitioners.
Effect of physical rehabilitation programs on the recovery sensorimotor functions in premature infants during the postnatal ontogenes’s period
Authors: Nalobina A. N. , Volova M. V. , Dakuko A. N. 
Published: August 2021

Introduction.Improving the treatment of premature infants is one of the priorities in the development of perinatal care for children in Russia. The widespread introduction of modern high-tech methods of resuscitation of premature children has shown its effectiveness in improving their survival. However this has led to an increase in various health disorders. Therefore, it is extremely important to determine and practically use effective technologies of medical care that will not only save the lives of children born prematurely, but also significantly affect their health indicators in subsequent age periods.Purpose of the study. To study the influence of various physical rehabilitation programs on the development of adaptive reactions and correction of impaired sensorimotor functions in early postnatal ontogenesis in premature infants.Materials and methods.A comprehensive study of 120 infants in the first year of life was carried out, including functional testing, anthropometric, general clinical and physiological methods for the development and assessment of the impact of physical rehabilitation programs of different volume and content on sensorimotor development and the state of adaptive capabilities of the body of premature infants in the first year of life.Results.The maximum effectiveness of rehabilitation measures for premature infants of the first year of life was noted only with a combination of sympathetic type of autonomic regulation of the heart rate and a program that included therapeutic exercises, massage, dry immersion and hydrokinesitherapy. In premature babies with a pronounced predominance of sympathetic regulation of the heart rhythm, the smallest rehabilitation programs are most appropriate.Conclusions. When developing a physical rehabilitation program, it is necessary to take into account the state of the adaptive capabilities of the body of premature infants in the first year of life, which can be determined by the type of autonomic regulation of the heart rate.
Optimization of the method for diagnostics of cardial autonomic neuropathy in diabetes mellitus 2 types
Authors: Moshkhoeva L. S. , Barinov A. N. 
Published: August 2021

Introduction. Early diagnosis of cardiac autonomic neuropathy is the basis for the risk of heart disease and premature death in patients with type 2 diabetes mellitus (type 2 diabetes). All patients with type 2 diabetes and prediabetes should undergo annual screening, including history taking and neurophysiologic evaluation, for early diabetic autonomic neuropathy. This article describes a modern diagnostic technique based on changes in heart rate variability, which allows early detection of damage to the parasympathetic nervous system and timely prescribing disease-modifying treatment in patients with type 2 diabetes.Materials and methods. The study included 50 patients with type 2 diabetes (cf. Age 56.5 ± 13.5 years) and 30 healthy subjects (cf. Age 58 ± 10 years), matched by sex and age. according to the NSC questionnaire, the severity of neurological deficit - according to the NIS-LL scale, neuropathic pain syndrome - according to the TSS scale.Results. In the study group of patients with type 2 diabetes, severe CAN was detected in 13.3%, moderate CAN in 40% of patients, in 46.6% of patients, CAN was not detected. In the group of healthy volunteers, disorders of the autonomic nervous system of moderate were detected in 3.3%, in the remaining 96.7% of autonomic disorders were not accepted.Conclusion. The results of pulse oximetric tests with deep breathing are comparable to the results of the QAT device Case-4 (p < 0.001). The method of pulse oximetric diagnostics is more practical to use in comparison with the hardware QAT.
Design of a monitoring network: from macro to micro design
Authors: Lepot Mathieu , Kapelan Zoran , Clemens-Meyer Francois H. L. R. 
Published: August 2021

Abstract
Designing a monitoring network or a measuring set-up or a monitoring station is a typical (multidisciplinary) engineering enterprise: a range of potentially conflicting demands (technical, financial and managerial) and limitations (e.g. availability of resources, skilled personnel, regulations) have to be respected. This chapter addresses the design aspects on both the macro scale (a monitoring network) and on the micro scale. The macro scale addresses what to measure, where to measure, how frequently to measure and the applications of models in the design process. On the micro scale issues with safety, accessibility and practical limitations are discussed. This chapter has close links with virtually all other chapters in this book and a comprehensive set of literature references is supplied to allow the interested reader to broaden his/her knowledge on the subject.
A maximum entropy copula-based frequency analysis method for assessing bivariate drought risk: a case study of the Kaidu River Basin
Authors: Yang X. , Li Y. P. , Huang G. H. 
Published: August 2021

Abstract
In this study, a maximum entropy copula-based frequency analysis (MECFA) method is developed through integrating maximum entropy, copulas and frequency analysis into a general framework. The advantages of MECFA are that the marginal modeling requires no assumption and joint distribution preserves the dependence structure of drought variables. MECFA is applied to assessing bivariate drought frequency in the Kaidu River Basin, China. Results indicate that the Kaidu River Basin experienced 28 drought events during 1958–2011, and drought inter-arrival time is 10.8 months. The average duration is 6.2 months (severity 4.6), and the most severe drought event lasts for 35 months (severity 41.2) that occurred from June 1977 to March 1980. Results also disclose that hydrological drought index (HDI) 1 is suitable for drought frequency analysis in the target year of return periods of 5 and 10, HDI 3, HDI 6 and HDI 12 are fit for the target year of return periods of 20, 50 and 100. The joint return period can be used as the upper bound of the target return period, and the joint return period that either duration or severity reaches the drought threshold can be used as the lower bound of the target return period.
Degradation of glyphosate in water by the application of surface corona discharges
Authors: Zocher Katja , Gros Peter , Werneburg Matthias , Brüser Volker , Kolb Juergen F. , Leinweber Peter 
Published: August 2021

Abstract
Glyphosate (GLP) is one of the most widely applied herbicides, and is found ubiquitously in the environment. The removal of glyphosate from waste water and soil is challenging and can be achieved with chemical or biological methods, which, nevertheless, suffer from different disadvantages. The application of a physical plasma for the removal of GLP in water was examined by the application of surface corona discharges in a wire-to-cylinder setup filled with argon. The plasma was ignited at the liquid surface without any additives. By applying a photometric method, GLP was detected after derivatisation with fluorenyl methoxycarbonyl chloride, whereas phosphate was determined with ammonium molybdate. A GLP degradation rate of 90.8% could be achieved within a treatment time of 30 minutes with an estimated energy efficiency of 0.32 g/kWh.
High Throughput Screening for Drug Discovery and Virus Detection
Authors: Chen Xin , Oke Adetola , Sahin Deniz , Shang Ying 
Published: August 2021

Background:
High throughput screening systems are automated labs for the analysis of
many biochemical substances in the drug discovery and virus detection process. This paper was
motivated by the problem of automating testing for viruses and new drugs using high throughput
screening systems. The emergence of severe acute respiratory syndrome coronavirus 2 (SARSCoV-
2) at the turn of 2019-2020 presented extraordinary challenges to public health. Existing
approaches to test viruses and new drugs do not use optimal schedules and are not efficient.

Objective:
The scheduling of activities performed by various resources in a high throughput
screening system affects its efficiency, throughput, operations cost, and quality of screening. This
study aims to minimize the total screening (flow) time and ensure the consistency and quality of
screening.

Methods:
This paper develops innovative mixed-integer models that efficiently compute optimal
schedules for screening many microplates to identify new drugs and determine whether samples
contain viruses. The methods integrate job-shop and cyclic scheduling. Experiments are conducted
for a drug discovery process of screening an enzymatic assay and a general process of detecting
SARS-CoV-2.

Results:
The method developed in this article can reduce screening time by as much as 91.67%.

Conclusion:
The optimal schedules for high throughput screening systems greatly reduce the total
flow time and can be computed efficiently to help discover new drugs and detect viruses.
Sensitivity Level of Placenta Accreta Index (PAI) Score and Placenta Accreta
Spectrum (PAS) Stage as Preoperative Diagnostic Tools for Placenta
Accreta Spectrum Disorders (PASD) at Haji Adam Malik General Hospital
Medan Indonesia
Authors: Purnama Utari , Sitepu Makmur , Edianto Deri , Lumbanraja Sarma Nursani , Sudewo Yudha , Simanjuntak Roy Yustin , Lubis Muara Panusunan , Barus Melvin Nova Gunawanto 
Published: August 2021

Background:
The incidence of Placenta Accreta Spectrum Disorders (PASD) has increased
by 10-fold in 50 years along with the number of cesarean sections. Ultrasound examination
using Placenta Accreta Index (PAI) score and Placenta Accreta Spectrum (PAS) stage as a predictor
of PASD has been used worldwide at the antenatal screening. The high diagnostic value of these
tools will help the physician to diagnose PASD early and minimize the rate of maternal neonatal
mortality and morbidity.

Objectives:
To evaluate the value of PAI score and PAS stage in diagnosing PASD.

Methods:
This study is a diagnostic test study using the medical records of mothers who gave birth
at Haji Adam Malik General Hospital Medan Indonesia between September 2017 to September
2020, who were diagnosed preoperatively as placenta previa suspected PASD through ultrasound
examination using PAI score or PAS stage. The results of these two diagnostic tests were compared
to clinical diagnostic criteria of PASD from The International Federation of Obstetrics and
Gynecology (FIGO) with or without histopathological confirmation.

Results:
Of the 177 placenta previa cases, there were 142 women with PASD (80.2%). The diagnostic
values of PAI score with 4.6 as an optimal cut-off point were 75% sensitivity, 83% specificity,
94% positive predictive values (PPV), and 47% negative predictive values (NPV). The diagnostic
values of the PAS stage were 90% sensitivity, 83%, specificity, 96% PPV, and 68% NPV.

Conclusion:
PAI score and PAS stage have a diagnostic value that looks equally good when used
as a diagnostic tool for PASD.
Gemcitabine-Related Atrial Fibrillation: A Case Report and Review of the
Literature
Authors: Abdallah Ichrak B. , Nasr Sonia Ben , Chourabi Chadia , Zribi Aref , Balti Mehdi , Fehri Wafa , Haddaoui Abderrazek 
Published: August 2021

:
Gemcitabine is a commonly used antimetabolite that has been effective in a broad spectrum
of tumors so far. The main grade three and four known toxicity of this drug is myelosuppression.
Cardiac adverse events have been rarely reported and gemcitabine-induced Atrial-Fibrillation
(AF) has been described in only five previous cases so far. Here we report the 6th case of gemcitabine-
related AF. A 68-year-old man diagnosed with metastatic nasopharyngeal cancer was referred
to our oncology department. He started first-line chemotherapy with gemcitabine and cisplatin.
He presented poorly tolerated atrial fibrillation related to gemcitabine infusion that lasted
for six days. The treatment was then withdrawn, and the patient received the best supportive care.
We conclude that medical oncologists and cardiologists should be aware of such toxicities of gemc-
itabine, especially in the elderly who seem to be at a higher risk of such adverse events and
which may dictate discontinuation of the drug.
An Ayurvedic Based Dermal Treatment for Skin Sanitization
Authors: Wal Pranay , Wal Ankita , Pal Rashmi Saxena , Pal Yogendra , Saraswat Nikita 
Published: August 2021

Background:
Due to the prevailing coronavirus crisis, the use of sanitizers has become an urgent need of today to take preventive care as we step out for various reasons. Many brands and varieties of sanitizers are available in the market today.

Objective:
The present study has been undertaken to develop and analyse the effects of an Ayurvedic preparation to overcome all the ill effects exerted on the skin by the chemical-based sanitizers. As there are very bad long-lasting effects on skin after continuous use of synthetically derived sanitizers. This pandemic situation has forced all of us for using sanitizers at least 10 times a day, especially for children and the Geriatric population.

Material and Methods:
Formulation was developed with the help of natural ingredients and then it was evaluated on the safety and efficacy standards to make sure about the credibility of the designed preparation. The benefits of using this formulation were observed during the health camps organised by Diabport health care at various parks in Kanpur. The texture of the skin was improved and apart from this. This was assessed with the help of a questionnaire (KAP) which was developed and validated.

Results:
The herbal based sanitizer, derived from Ayurvedic principles, was found to be quite effective against germs, microbes and it was beneficial for the care of hands also. Hands were soft and irritation free when the herbal sanitizers were applied for optimum time. We have observed that those patients who have history of high sugar level are more prone of developing rashes due to excessive usage of sanitizers. Diabetic dermopathy was also relieved. The population which used this formulation showed sign of improvement in both the texture of skin as well as decreased number of rashes.

Conclusion:
Herbal-based sanitizers are the need of the hour to combat the danger of corona, keeping the hands mild and soft. More focus on them should be encouraged on a large scale.
Interventions to Reduce Patient Identification Errors in the Hospital Setting: A Systematic Review
Authors: De Rezende Helena , Melleiro Marta M. , O. Marques Paulo A. , Barker Timothy H. 
Published: August 2021

Background:
Patient identification is considered as a fundamental part of the care process and a relevant resource for safety practices in hospital settings.

Objective:
We aimed to review the literature on interventions to reduce patient identification errors in hospital settings.

Methods:
A systematic review of effectiveness using The Joanna Briggs Institute (JBI) methodology was conducted. A three-step search strategy was utilised to explore primary research published up to March 2020 in English, Spanish, and Portuguese across eight databases. Grey literature was also assessed. The titles and abstracts of the studies were screened for assessment of the inclusion criteria. Two reviewers independently appraised the full text of the selected studies and extracted data using standardised tools from JBI. Due to the heterogeneity of studies and insufficient data for statistical pooling, meta-analysis was not feasible. Therefore, the results were synthesised narratively.

Results:
Twelve studies met the review criteria; all were rated at a moderate risk of bias and four different groups of interventions were identified: educational staff interventions alone and those combined with a partnership with families and patients through education; and information technology interventions alone, and combined with an educational staff strategy. Although most studies showed a statistically significant reduction in patient identification errors, the overall quality of the evidence was considered very low.

Conclusion:
High-quality research is needed to understand the real impact of interventions to reduce patient identification errors. Nurses should recognise the importance of patient identification practices as a part of their overall commitment to improving patient safety.
PROSPERO Registration Number: CRD42018085236