bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonsense-associated alternative splicing as a putative reno-protective mechanism in Pkhd1cyli/Pkhd1cyli mutant mice
Authors: Yang Chaozhe , Harafuji Naoe , Odinakachukwu Maryanne C. , Caldovic Ljubica , Boddu Ravindra , Gordish-Dressman Heather , Foreman Oded , Eicher Eva M. , Guay-Woodford Lisa M. 
Published: September 2021

AbstractAutosomal recessive polycystic kidney disease (ARPKD) is a hereditary hepato-renal fibrocystic disorder and a significant genetic cause of childhood morbidity and mortality. Mutations in the Polycystic Kidney and Hepatic Disease 1 (PKHD1) gene cause all typical forms of ARPKD. Several mouse strains carrying diverse genetically engineered disruptions in the orthologous Pkhd1 gene have been generated. The current study describes a novel spontaneous mouse recessive mutation causing a cystic liver phenotype resembling the hepato-biliary disease characteristic of human ARPKD. Here we describe mapping of the cystic liver mutation to the Pkhd1 interval on Chromosome 1 and identification of a frameshift mutation within Pkhd1 exon 48 predicted to result in premature translation termination. Mice homozygous for the new mutation, symbollzed Pkhd1cyli, lack renal pathology, consistent with previously generated Pkhd1 mouse mutants that fail to recapitulate human kidney disease. We have identified a profile of alternatively spliced Pkhd1 renal transcripts that are distinct in normal versus mutant mice. The Pkhd1 transcript profile in mutant kidneys is consistent with predicted outcomes of nonsense-associated alternative splicing (NAS) and nonsense mediated decay (NMD). Overall levels of Pkhd1 transcripts in mutant mouse kidneys were reduced compared to kidneys of normal mice, and Pkhd1 encoded protein in mutant kidneys was undetectable by immunoblotting. We suggest that in Pkhd1cyli/Pkhd1cyli(cyli) mice, mutation-promoted Pkhd1 alternative splicing in the kidney yields transcripts encoding low-abundance protein isoforms lacking exon 48 encoded amino acid sequences that are sufficiently functional so as to attenuate expression of a renal cystic disease phenotype.
Synthesis, spectroscopic characterization, structural studies, thermal analysis and molecular docking ofN-(2-methyl-5-nitrophenyl)-4-(pyridin-2-yl)pyrimidin-2-amine, a precursor for drug design against chronic myeloid leukemia
Authors: Moreno-Fuquen Rodolfo , Arango-Daraviña Kevin , Kennedy Alan R. 
Published: September 2021

The synthesis, crystal structure and spectroscopic and electronic properties ofN-(2-methyl-5-nitrophenyl)-4-(pyridin-2-yl)pyrimidin-2-amine (NPPA), C16H13N5O2, a potential template for drug design against chronic myelogenous leukemia (CML), is reported. The design and construction of the target molecule were carried out starting from the guanidinium nitrate salt (previously synthesized) and the corresponding enaminone. X-ray diffraction analysis and a study of the Hirshfeld surfaces revealed important interactions between the nitro-group O atoms and the H atoms of the pyridine and pyrimidine rings. A crystalline ordering in layers, by the stacking of rings through interactions of the π–π type, was observed and confirmed by a study of the shape-index surfaces and dispersion energy calculations. Quantitative electrostatic potential studies revealed the most positive value of the molecule on regions close to the N—H groups (34.8 kcal mol−1); nevertheless, steric impediments and the planarity of the molecule do not allow the formation of hydrogen bonds from this group. This interaction is however activated when the molecule takes on a new extended conformation in the active pocket of the enzyme kinase (PDB ID 2hyy), interacting with protein residues that are fundamental in the inhibition process of CML. The most negative values of the molecule are seen in regions close to the nitro group (−35.4 and −34.0 kcal mol−1). A molecular docking study revealed an energy affinity of ΔG = −10.3 kcal mol−1forNPPAwhich, despite not having a more negative value than the control molecule (Imatinib; ΔG = −12.8 kcal mol−1), shows great potential to be used as a template for new drugs against CML.
SWOT analysis of Industry 4.0 variables using AHP methodology and structural equation modelling
Authors: Jain Vineet , Ajmera Puneeta , Davim João Paulo 
Published: September 2021

PurposeAdvanced digitalization techniques combined with artificial intelligence and automated robotic systems have created “Smart” organizations resulting in a new revolution in the industrial production systems as Industry 4.0 (I4.0). The research is aimed to do a meticulous scanning of internal and external environment pertaining to I4.0 implementation in the manufacturing industry in India.Design/methodology/approachA survey was conducted among the manufacturing managers and information technology professionals about the factors affecting I4.0 application, and 20 such internal and external factors were identified. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were executed for factor analysis, and four dimensions in terms of strengths, weaknesses, opportunities and threats (SWOT) factors were determined from the variables. The analytical hierarchy process (AHP) methodology was then applied.FindingsResults show that increased productivity and efficiency appeared to be the biggest strength of I4.0 while the biggest weakness is the need for specialized training and skills. The biggest opportunity is found to be increasing trust of customers in Internet transactions and employee resistance to adopting new technologies turned out to be the biggest threat.Practical implicationsOrganizations will be able to evaluate the strengths, work upon weakness, exploit the opportunities and protect against external challenges and threats beforehand while implementing I4.0 technologies.Originality/valueThe four dimensions in terms of SWOT pertaining to manufacturing industry have been identified by collecting original data from the manufacturing industry, and AHP and CFA were then carried out to prioritize and verify them.
Spatial-temporal analysis for the business performance of construction consultancy services in China
Authors: Hu Xiancun , Hu Xianhu , Cui Qinghong , Li Yan , Lemckert Charles , Wei Aifang 
Published: September 2021

PurposeThis paper presents a developed spatial-temporal analysis framework for the case of investigating the business performance of construction consultancy services (CCS) in China.Design/methodology/approachThe spatial-temporal analysis is based on the data envelopment analysis (DEA) technique. The spatial analysis follows the DEA results under a contemporaneous benchmark technology and a virtual decision-making unit, consisting of ranking analysis, cluster analysis and variation analysis. The temporal analysis is reliant on the DEA results under a global benchmark technology and the time value of money, including trend analysis and driving force analysis containing pure technical and scale efficiency factors.FindingsThree CCS types in China are investigated, including engineering survey and design, construction supervision and procurement agency. The performance rank order and cluster classifications are mainly related to economic development levels. Engineering survey and design demonstrates the best performance and higher imbalances; however, construction supervision and procurement agency illustrate lower performance and imbalances. Scale efficiency significantly promotes business performance, whereas pure technical efficiency plays an inconspicuous role.Practical implicationsThe CCS promote technical efficiency by developing their service and innovation levels. The service of engineering survey and design registered in Beijing, Shanghai and Guangdong is recommended for entering the service market in China.Originality/valueThe spatial-temporal analysis framework was developed, which is generic and provides a pathway to measure, compare and assess performance comprehensively. The CCS business performance is firstly measured.
Building information modeling to support facilities management of large capital projects: a critical review
Authors: Asare Kofi Asabere Bediako , Liu Rui , Anumba Chimay J. 
Published: September 2021

Purpose
This paper aims to present a review of research developments relating to the application of building information modeling (BIM) to facilities management (FM). It sheds light on major technical and organizational issues with the view of identifying how existing BIM for FM knowledge applies to large capital projects.

Design/methodology/approach
The study adopted a literature search approach to retrieve relevant articles which were subjected to keyword analysis to enable categorization of extant BIM for FM research into appropriate sub-domains. A qualitative analysis of 94 BIM for FM-related literature was carried out in addition to a review of 9 capital project-related articles, leading to the establishment of research trends, gaps and future directions.

Findings
The review found that research in the BIM-FM integration field is predominantly technology and process-oriented, with less attention paid to people or organizational aspects. Therefore, there is a need for expanding the knowledge base in this direction. Several future research directions were identified to lay the foundations for research on BIM application to FM in large capital projects and other application areas for interested researchers. These future directions were categorized under the identified sub-domains of the field and mapped onto two generic activities, i.e. technical integration and business integration, involved in technology adoption by organizations.

Originality/value
The main contribution of this study is the categorization of existing research on BIM for FM, leading to the identification of research gaps concerning the initiation and implementation of BIM for FM in large capital projects. As a secondary benefit, this study has validated some sub-domains of the BIM for the FM research field identified in previous review papers using an empirical approach. This validation of defined sub-domains is useful for an emerging research field as it provides a common understanding of trends and specific application areas.
Development of theory of mind: importance of nurturance
Authors: Pullangotte Anagha , Mangadu Paramasivam Ganesh 
Published: September 2021

PurposeTheory of mind (ToM) is essential in understanding and predicting human behaviour. Parenting plays a significant role in the overall cognitive development of children. This study aims to understand the development of ToM among children in need of care and protection and then to compare the data with children living under parental care and children living in boarding schools. Further, it explores the extent of physical abuse experienced by children in the study and their relation to the development of ToM.Design/methodology/approachToM Test developed by Muris et al. (1999) was used to measure ToM. Childhood Experience of Care and Abuse Questionnaire was used to understand the children’s relationship with parents and experience of physical abuse. The study used an ex post facto design with a purposive sampling method.FindingsFindings suggest a significant impact of parental care on the ToM among children. Also, the type of care received mediated the relationship between parental care and the development of ToM. Finally, children living in institutions run by the Child Welfare Department reported that they have received harsher physical punishment from their parents than the other two groups of children.Research limitations/implicationsFindings are a significant theoretical contribution to the ToM development in children, especially in the Indian context.Social implicationsFindings demand more legal and psychological support to vulnerable children living in institutions run by the Child Welfare Department and boarding schools.Originality/valueThe study explores care and abuse from the child’s perspective. Findings are of value to the existing child care system in India.
Chinese fintech companies and their “going out” strategies
Authors: Han Alice Siqi 
Published: September 2021

PurposeThis paper outlines the rapid rise of China's fintech companies over the past decade with a focus on their globalization strategies as they enter their next phase of development.Design/methodology/approachThe author examines China's current and prospective influence on global financial digitization trends, and assesses both domestic and foreign opportunities and challenges confronted by China's fintech firms as they look to expand abroad.FindingsThe Chinese government is experimenting with a radically new fintech system and a regulatory regime in response to it. Chinese ambitions to expand fintech influence through private companies and the state-led “digital RMB” (e-CNY) will likely provoke a wave of “digital protectionism” among developed nations to protect internal digital payments.Originality/valueThis paper is an original economic history research on China's fintech industry.
Entrepreneurial ecosystems quality and productive entrepreneurship: entrepreneurial attitude as a mediator in early-stage and high-growth activities
Authors: Kansheba Jonathan Mukiza , Wald Andreas Erich 
Published: September 2021

PurposeThis study examines the mediation effects of entrepreneurial attitudes (EAs) on the nexus of the entrepreneurial ecosystem (EE) quality and productive entrepreneurship for early-stage and high-growth entrepreneurial activities.Design/methodology/approachThe study employs global entrepreneurship monitor (GEM) panel data of 137 economies from 2014 to 2018. Random effect panel regressions and relative effect size estimations were used for data analysis.FindingsThe study’s findings show complementary mediation effects suggesting that EE quality steers entrepreneurial activities via the EA. However, such mediation is much more vivid towards high growth than early-stage activities. Vibrant EEs provide necessary resources that boost the attitude of potential and nascent entrepreneurs to engage in early stage and high-growth entrepreneurial activities.Research limitations/implicationsThe study utilizes GEM data to explain the EEs and EA dynamics and their related effects on entrepreneurship at the macro level. Future research may study the phenomena by using micro level data.Originality/valueThe paper explores a less empirically researched question on how EEs steer entrepreneurship growth and development. It reveals a need for new perspectives/logics (e.g. mediation/moderation) for improving the explanations on the extant EEs framework. It further informs policymakers and practitioners to design entrepreneur-centred EE policies and programs.
Exploring determinants influencing information literacy with activity theory
Authors: Li Xuanxi , Islam A.Y.M. Atiquil , Cheng Eddie W.L. , Hu Xiao , Wah Chu Samuel Kai 
Published: September 2021

PurposeThis study aimed to provide evidence to support the use of a wiki called PBworks as a learning tool to foster students' information literacy (IL) skills based on activity theory.Design/methodology/approachThe participants consisted of 421 students (i.e. form 1 to form 3) from Hong Kong taking a liberal studies course during the 2016–2017 academic year. This study mainly used a mixed methods design, proposing 11 hypotheses. Quantitative data from 374 questionnaires were analysed to test these research hypotheses, while a qualitative method (interviews) was used to explain the quantitative results. A structural equation modelling approach was used to analyse the data, and data triangulation was used to answer the same research questions.FindingsThe results showed that the model components PBworks affordances (PB) and rules and divisions (RD) had significant direct effects on individual activities (IA) and community activities (CA) and significant indirect effects on information literacy (IL). The results also revealed that CA had a significant effect on IA and had an even greater effect on IL.Research limitations/implicationsUsing PBworks and the project-based learning (PjBL) approach, this study examined the determinants affecting the IL skills of Hong Kong junior secondary school students and proposed a wiki-based information literary activity (WILA) model.Practical implicationsAs students' IL skills have become increasingly important, this study can shed light on related topics for future studies.Social implicationsAnd contribute to social stability and harmonious development.Originality/valueThis study eventually confirmed the validity of the WILA model with all hypotheses supported.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/OIR-03-2020-0092.
Mapping haemodynamic changes with rapid sequence induction agents in the emergency department
Authors: Freeman Jessica , Alkhouri Hatem , Knipp Robert , Fogg Toby , Gillett Mark 
Published: September 2021

AbstractObjectivePatients intubated in the ED are at an increased risk of post‐intubation hypotension. However, evidence regarding the most appropriate induction agent is lacking. The present study aims to describe and compare the haemodynamic effect of propofol, ketamine and thiopentone during rapid sequence induction.MethodsThis is an observational study using data prospectively collected from the Australian and New Zealand Emergency Department Airway Registry between June 2012 and March 2019. The distribution of induction agents across medical and trauma patients were obtained with descriptive statistics. The relationship between induction agent, dose and change in pre‐ and post‐intubation systolic blood pressure (SBP) was described using multivariable logistic regression. The SBP pre‐ and post‐intubation was the primary measure of haemodynamic stability.ResultsFrom the 5063 intubation episodes, 2229 met the inclusion criteria. Of those, 785 (35.2%) patients were induced with thiopentone, 773 (34.7%) with propofol and 671 (30.1%) with ketamine. Of the included population, 396 (17.8%) patients experienced a reduction in pre‐intubation SBP exceeding 20%. Both propofol (P = 0.01) and ketamine (P = 0.01) had an independent and dose‐dependent association with hypotension, noting that a higher proportion of patients induced with ketamine had a shock index exceeding 0.9.ConclusionPropofol was associated with post‐intubation hypotension and it is recommended clinicians consider using the lowest effective dose to reduce this risk. Reflecting its perceived haemodynamic stability, patients who received ketamine were more likely to have a higher shock index; however, there was also an association with post‐intubation hypotension.
Decolonisation, knowledge production, and interests in liberal higher education
Authors: Rangan Haripriya 
Published: September 2021

AbstractThis essay explores how calls for decolonisation in universities have engaged with ideas about liberalism and liberal education. It maps the historical context of liberal education as embodied in the development of modern European universities and colonial interests in their respective nation‐states. It offers comparative perspectives on how ideas of decolonisation in higher education have confronted liberal and nation‐state interests at different historical conjunctures in three postcolonial settings: in the establishment of a national university in India during the 1960s; in protests by university students in postapartheid South Africa between 2015 and 2017; and in growing commitments to Indigenous recognition and knowledges in Australian universities from the 2000s. The essay highlights the need for those active in contemporary decolonisation movements in universities in the global North and South to confront the paradoxical interests of liberal education and nationalisms in knowledge production, epistemic and socio‐economic justice, and sociality in higher education.Key insightsDespite enthusiasm about decolonisation in/of academic debates, few have sought to critically analyse its relationship to ideas of liberalism and liberal education in universities. This essay considers how ideas of decolonisation in higher education have confronted liberal and nation‐state interests at different historical moments in India, South Africa, and Australia. The essay highlights the need to confront the paradoxical interests of liberal education and nationalisms in knowledge production, epistemic and socio‐economic justice, and sociality in higher education.
Late‐in‐life treadmill training rejuvenates autophagy, protein aggregate clearance, and function in mouse hearts
Authors: Cho Jae Min , Park Seul‐Ki , Ghosh Rajeshwary , Ly Kellsey , Ramous Caroline , Thompson Lauren , Hansen Michele , Mattera Maria Sara de Lima Coutinho , Pires Karla Maria , Ferhat Maroua , Mookherjee Sohom , Whitehead Kevin J. , Carter Kandis , Buffolo Márcio , Boudina Sihem , Symons J. David 
Published: September 2021

AbstractProtein quality control mechanisms decline during the process of cardiac aging. This enables the accumulation of protein aggregates and damaged organelles that contribute to age‐associated cardiac dysfunction. Macroautophagy is the process by which post‐mitotic cells such as cardiomyocytes clear defective proteins and organelles. We hypothesized that late‐in‐life exercise training improves autophagy, protein aggregate clearance, and function that is otherwise dysregulated in hearts from old vs. adult mice. As expected, 24‐month‐old male C57BL/6J mice (old) exhibited repressed autophagosome formation and protein aggregate accumulation in the heart, systolic and diastolic dysfunction, and reduced exercise capacity vs. 8‐month‐old (adult) mice (all p < 0.05). To investigate the influence of late‐in‐life exercise training, additional cohorts of 21‐month‐old mice did (old‐ETR) or did not (old‐SED) complete a 3‐month progressive resistance treadmill running program. Body composition, exercise capacity, and soleus muscle citrate synthase activity improved in old‐ETR vs. old‐SED mice at 24 months (all p < 0.05). Importantly, protein expression of autophagy markers indicate trafficking of the autophagosome to the lysosome increased, protein aggregate clearance improved, and overall function was enhanced (all p < 0.05) in hearts from old‐ETR vs. old‐SED mice. These data provide the first evidence that a physiological intervention initiated late‐in‐life improves autophagic flux, protein aggregate clearance, and contractile performance in mouse hearts.
A justification of health policy federalism
Authors: Weinstock Daniel 
Published: September 2021

AbstractThe apportionment of responsibility for health policy within multi‐level states should be sensitive to a number of conflicting normative pressures, some of which militate for placing decision‐making authority at the higher reaches of policy‐making structures, while others would seem to require placing them lower down this structure. The principle of subsidiarity is a structural principle that addresses in a manner that is neutral with respect to these values a way of addressing the conflicting claims of these values. Standard accounts of federalism fare poorly with respect to the criterion of subsidiarity. While central governments are at first glance better equipped to apply such a principle to the issue of the distribution of authority, there are strong empirical grounds for thinking that centralized governments will non‐neutrally privilege central authorities in applying the principle. Federal structures that admit of overlapping jurisdictions, and that therefore require that deliberation among federal parties occur as a condition of the problem of the distribution of powers over health care being solved, are most amenable to solving problems of distribution of authority.