bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay
using Clinical Samples in China
Authors: Gao Peijie , Yu Fengting , Yang Xiaozhen , Li Dan , Shi Yalun , Wang Yan , Zhang Fujie 
Published: September 2021

Background:
HIV drug resistance poses a major challenge for anti-retroviral treatment
(ART) and the prevention and control of HIV epidemic.

Objective:
The study aims to establish a novel in-house assay with high efficiency, named AP inhouse
method, that would be suitable for HIV-1 drug resistance detection in China.

Methods:
An in-house HIV-1 genotyping method was used to sequence the partial pol gene from
60 clinical plasma samples; the results of our test were compared with a commercial ViroSeq
HIV-1 genotyping system.

Results :
Among sixty samples, 58(96.7%) were successfully amplified by AP in-house method,
five of them harbored viral load below 1,000 copies/ml. The genotype distribution was 43.1% CRF07_
BC (25/58), 39.7% CRF01_AE (23/58), 6.9% CRF55_01B (4/58), 5.2% subtype B (3/58) and
5.2% CRF08_BC (3/58). Compared with that of the ViroSeq system, the consistent rate of these nucleotides
and amino acids obtained by AP in-house method was up to 99.5 ± 0.4% and 99.5 ±
0.4%, respectively. A total of 290 HIV-1 drug resistance mutations were identified by two methods,
including 126 nucleoside reverse transcriptase inhibitors (NRTIs), 145 non-nucleoside reverse
transcriptase inhibitors (NNRTIs) and 19 protease inhibitors (PIs) resistance mutations. Out of
them, 94.1% (273/290) were completely concordant between the AP in-house method and the ViroSeq
system.

Conclusion:
Overall, the evaluation of AP in-house method provided comparable results to those
of the ViroSeq system on diversified HIV-1 subtypes in China.
A Critical Scientific Review on Withania somnifera, Garcinia cambogia and
Curcumin Supplements: Food Forensics Perspective
Authors: Behl Akanksha , Mishra Amarnath , Sharma G. P. 
Published: September 2021

Abstract:
Food forensics is an emerging branch that applies scientific knowledge for the verification
and authentication of food supplements. The term “dietary supplement” refers to a diverse
set of preparations that are deemed to be essential for the well-being of the human body.
These supplements consist of various physiologically active substances that produce effects on
the body. However, a major issue with these supplements is that they are neither considered as
medicines nor as a food products. Due to this, such food supplements are prone to adulteration
and counterfeiting. In this review, three widely used supplements, namely Curcuma longa,
Withania somnifera, and Garcinia cambogia supplements, are considered. Chemistry, pharmacology,
and benefits of three are being discussed along with the analytical techniques for their
analysis. Chromatographic techniques are popular for the examination of dietary supplements.
Several such analytical techniques are being used to detect and identify the physiologically active
constituents as well as adulterants in dietary products. This review intends to demonstrate
the overall issues regarding dietary supplements.
Investigating the Effect of Stress Management Counseling on the Sexual
Function of Infertile Women
Authors: Shayan Arezoo , Masoumi Seyedeh Zahra , Khodakarami Batul , Ahmadnia Hasan , Dastgerdian Fatemeh , Rabiei Sogra 
Published: September 2021

Background:
Sexual dysfunction is one of the most common causes of family breakdown.
In recent years, different approaches have been used to solve this problem. Due to the negative
effects of sexual dysfunction in women and its complications.

Objective:
The present study was conducted to investigate the effect of stress management counseling
on the sexual function of infertile women referred to Fatemieh Hospital in Hamadan.

Methods:
The present study was conducted as a randomized two-group clinical trial with two pretest
and post-test stages on 104 eligible women referring to Fatemieh Hospital in Hamadan in
2017-2018. Intervention group subjects received 4 two-hour group counseling sessions with a cognitive-
behavioral approach on sexual issues. Data were obtained using two questionnaires of demographic
characteristics and the Female Sexual Function Index. To analyze the data, SPSS version
21 software and paired t-test, independent t-test, and analysis of covariance were used. The level of
significance was considered to be less than 0.05.

Results:
The results of the present study showed that the mean scores in all areas of total sexual
function did not differ significantly before the intervention in two groups. However, after stress management
counseling, a significant increase was observed in all areas and general sexual function
in the intervention group compared to the control group (P <0.05).

Conclusion:
Stress management counseling in infertile women can play a positive and effective
role in improving sexual function. Therefore, the effectiveness of this counseling in promoting sexual
function can be confirmed with more confidence.

Registeration No:
The study was registered at the Iranian Registration Clinical Trials (IRCT201704149014N158)
by I IRCT.
Sports-Related Brain Injury and Neurodegeneration in Athletes
Authors: Dhote Vipin V. , Raja Muthu Kumaradoss Mohan Maruga , Samundre Prem , Sharma Supriya , Anwikar Shraddha , Upaganlawar Aman B. 
Published: September 2021

:Sports deserve a special place in human life to impart healthy and refreshing wellbeing. However, sports activities, especially contact sports, renders athlete vulnerable to brain injuries. Athletes participating in a contact sport like boxing, rugby, American football, wrestling, and basketball are exposed to traumatic brain injuries (TBI) or concussions. The acute and chronic nature of these heterogeneous injuries provides a spectrum of dysfunctions that alters the neuronal, musculoskeletal, and behavioral responses of an athlete. Many sports-related brain injuries go unreported, but these head impacts trigger neurometabolic disruptions that contribute to long-term neuronal impairment. The pathophysiology of post-concussion and its underlying mechanisms are undergoing intense research. It also shed light on chronic disorders like Parkinson's disease, Alzheimer's disease, and dementia. In this review, we examined post-concussion neurobehavioral changes, tools for early detection of signs, and their impact on the athlete. Further, we discussed the role of nutritional supplements in ameliorating neuropsychiatric diseases in athletes.
Teeth with Apical Periodontitis Obturated in the Presence or Absence of Cultivable Micro-Organisms: A Two-Year Prospective Study of Treatment Outcomes
Authors: Chu Frederick C.S. , Ding Xun , Chow Tak W. , Samaranayake Lakshman P. 
Published: September 2021

Objective:
This prospective study aims to evaluate the one-year and two-year outcomes
of root canals with apical periodontitis obturated in the presence or absence of cultivable micro-organisms,
and define the influence on endodontic treatment outcome from different independent
variables.

Methods:
87 patients with 45 exposed and 43 unexposed canals who previously participated in a
microbiological study of primary endodontic infections were invited back for review in 12 and 24
months after endodontic treatment. The review involves clinical examination, questionnaire interview
and radiographic assessment in order to determine the treatment outcomes. The influence of
independent variables, such as gender, age, tooth location, etc., on the treatment outcome, has also
been analysed.

Results:
A total of 85 teeth of 84 patients were reviewed one and two years after obturation. Chisquare
test for trend showed that the one-year radiographic healing of apical lesions was significantly
different between culture-positive and negative canals (P<0.05). However, there is no significant
difference in the two-year radiographic healing between the two groups of canals (P>0.05). None
of the independent variables was found to be significant in affecting the endodontic treatment outcome
on logistic regression analysis.

Conclusions:
The presence of positive culture prior to obturation seemed to affect the healing of periapical
tissues in a one-year review, but the effect appeared to be temporal and not affected the
long-term outcome.
Factors Affecting the Extent of Patients’ Electronic Medical Record Use: An Empirical Study Focusing on System and Patient Characteristics
Authors: Agrawal Lavlin , Ndabu Theophile , Mulgund Pavankumar , Sharman Raj 
Published: September 2021

Background
Patients’ access to and use of electronic medical records (EMRs) places greater information in their hands, which helps them better comanage their health, leading to better clinical outcomes. Despite numerous benefits that promote health and well-being, patients’ acceptance and use of EMRs remains low. We study the impact of predictors that affect the use of EMR by patients to understand better the underlying causal factors for the lower use of EMR.

Objective
This study aims to examine the critical system (eg, performance expectancy and effort expectancy) and patient characteristics (eg, health condition, issue involvement, preventive health behaviors, and caregiving status) that influence the extent of patients’ EMR use.

Methods
We used secondary data collected by Health Information National Trends Survey 5 cycle 3 and performed survey data analysis using structural equation modeling technique to test our hypotheses. Structural equation modeling is a technique commonly used to measure and analyze the relationships of observed and latent variables. We also addressed common method bias to understand if there was any systematic effect on the observed correlation between the measures for the predictor and predicted variables.

Results
The statistically significant drivers of the extent of EMR use were performance expectancy (β=.253; P<.001), perceived behavior control (β=.236; P<.001), health knowledge (β=–.071; P=.007), caregiving status (β=.059; P=.013), issue involvement (β=.356; P<.001), chronic conditions (β=.071; P=.016), and preventive health behavior (β=.076; P=.005). The model accounted for 32.9% of the variance in the extent of EMR use.

Conclusions
The study found that health characteristics, such as chronic conditions and patient disposition (eg, preventive health behavior and issue involvement), directly affect the extent of EMR use. The study also revealed that issue involvement mediates the impact of preventive health behaviors and the presence of chronic conditions on the extent of patients’ EMR use.
El impacto del trabajo de Mario Molina en los programas y materiales educativos de educación media y media superior en México
Authors: Catalá Rodes Rosa María , Chamizo José Antonio , García-Franco Alejandra 
Published: September 2021

<span id="docs-internal-guid-75f3ec9e-7fff-b612-d527-e223aeaf5f5f"><span>Se presentan los resultados y la discusión de una búsqueda a nivel curricular y metodológico del impacto que tuvieron los trabajos de Mario Molina en la educación preuniversitaria en México de 1986 a la fecha. Se encontraron evidencias de la importancia que se dio en un primer momento al tema de la desaparición de la capa de ozono en países donde la enseñanza tipo CTS (Ciencia, Tecnología y Sociedad) y la enseñanza en contexto empezaban a cobrar relevancia para incluir la dimensión ambiental en los planes y programas de estudio. En nuestro país la respuesta fue tibia, con apenas sugerencias de segundo plano para incluir la figura y el trabajo del químico mexicano más importante a nivel nacional e internacional. Los materiales educativos y de divulgación encontrados se presentan en forma de tablas para facilitar su rápida consulta y uso por parte de los docentes de secundaria y nivel medio superior con el fin de que estos se aprovechen en lecciones o secuencias didácticas que incorporen aspectos históricos y de reflexión sobre los retos ambientales que enfrentamos. Con ello se apuesta a que las generaciones actuales y futuras conozcan y reconozcan que la química que llevó al tratado de Montreal y el éxito de su aplicación, fue desarrollada por un científico cercano que confió en su trabajo y en el diálogo para buscar soluciones y quien dedicó además el resto de su vida a estudios sobre la contaminación del aire en las grandes ciudades y el cambio climático. </span></span>
La cadena productiva de mora en el Departamento Risaralda en el marco de un estudio de prospectiva a 2032
Authors: Suarez Lina Maria , Agudelo Diego Alejandro , Zartha Jhon Wilder , Orozco Gina Lía 
Published: September 2021

El presente artículo, describe las actividades y resultados de investigación del proyecto “ALIANZA REGIONAL PARA EL FORTALECIMIENTO DEL SECTOR AGROINDUSTRIAL EN RISARALDA” - Componente Prospectiva, mediante la aplicación del método Delphi, que consiste en una consulta a un número de expertos o grupos de interés del sector Mora, sobre la base de un cuestionario, que se responde anónimamente en dos rondas. El objetivo del estudio es ofrecer herramientas de decisión a los actores del sector productivo de mora, en temas relacionados con ciencia, tecnología e innovación, los cuales servirán para el fortalecimiento y desarrollo de esta cadena productiva en todos sus eslabones. Como resultados importantes, se resaltan la priorización de 26 temas en 6 agrupadores: mora (cultivo), transformación, logística, comercialización, poscosecha y sostenibilidad, destacándose nueve temas prioritarios para la segunda ronda Delphi: Manejo del cultivo de mora, prácticas de manejo del cultivo de mora, manejo de registros, costos de producción, producción (orgánica o limpia), poscosecha, colorantes, saborizantes, fuentes de financiamiento. Se resalta la participación de expertos internacionales de México, Brasil y España, donde se identificaron las apuestas a futuro de la cadena de la mora que se convierten en apuestas directas para los actores relacionados con I+D+i para la adquisición, adopción y generación de innovaciones de producto y de proceso, así como posibles actividades de formación e investigaciones desde las universidades con enfoque agroindustrial.
Modelo multicriterio para el diseño de cadenas de suministro considerando opciones reales para el tratamiento de la incertidumbre
Authors: Jimenez Carabali Victor Javier , Manotas Duque Diego Fernando , Rivera Cadavid Leonardo 
Published: September 2021

Los problemas relacionados con la configuración de cadenas de suministros incluyen variables cuantitativas y cualitativas. Entre los atributos cuantitativos el más común es el dinero, y muchos atributos como la utilización de las máquinas, la fuerza de trabajo y la calidad pueden traducirse en términos monetarios. Algunos ejemplos de variables cualitativas son el estado de ánimo de las personas, la satisfacción del cliente, la reputación en la comunidad, la apariencia, la estética, etc. Los modelos de decisión de atributos múltiples integran los atributos cuantitativos y cualitativos para producir una medida de desempeño agregada En este artículo se propone un modelo de decisión multicriterio utilizando basado en el Analytical Hierarchy Procces (AHP) para la configuración de cadenas de abastecimiento integrando elementos de flexibilidad financiera a través del análisis de opciones reales. El modelo desarrollado contempla elementos de riesgo financiero a partir de la consideración de variables como demanda, tasas de cambio y tasas de interés, y permite valorar la flexibilidad operativa del proyecto ante cambios en dichos elementos de riesgo.
The Bihormonal Bionic Pancreas Improves Glycemic Control in Individuals With Hyperinsulinism and Postpancreatectomy Diabetes: A Pilot Study
Authors: Rayannavar Arpana , Mitteer Lauren M. , Balliro Courtney A. , El-Khatib Firas H. , Lord Katherine L. , Hawkes Colin P. , Ballester Lance S. , Damiano Edward R. , Russell Steven J. , De León Diva D. 
Published: September 2021

OBJECTIVE
To determine whether the bihormonal bionic pancreas (BHBP) improves glycemic control and reduces hypoglycemia in individuals with congenital hyperinsulinism (HI) and postpancreatectomy diabetes (PPD) compared with usual care (UC).

RESEARCH DESIGN AND METHODS
Ten subjects with HI and PPD completed this open-label, crossover pilot study. Coprimary outcomes were mean glucose concentration and time with continuous glucose monitoring (CGM) glucose concentration &lt;3.3 mmol/L.

RESULTS
Mean (SD) CGM glucose concentration was 8.3 (0.7) mmol/L in the BHBP period versus 9 (1.8) mmol/L in the UC period (P = 0.13). Mean (SD) time with CGM glucose concentration &lt;3.3 mmol/L was 0% (0.002) in the BHBP period vs. 1.3% (0.018) in the UC period (P = 0.11).

CONCLUSIONS
Relative to UC, the BHBP resulted in comparable glycemic control in our population.
Minimum Threshold of Bariatric Surgical Weight Loss for Initial Diabetes Remission
Authors: Barthold Douglas , Brouwer Elizabeth , Barton Lee J. , Arterburn David E. , Basu Anirban , Courcoulas Anita , Crawford Cecelia L. , Fedorka Peter N. , Fischer Heidi , Kim Benjamin B. , Mun Edward C. , Murali Sameer B. , Reynolds Kristi , Yoon Tae K. , Zane Robert E. , Coleman Karen J. 
Published: September 2021

OBJECTIVE
There are few studies testing the amount of weight loss necessary to achieve initial remission of type 2 diabetes mellitus (T2DM) following bariatric surgery and no published studies with use of weight loss to predict initial T2DM remission in sleeve gastrectomy (SG) patients.

RESEARCH DESIGN AND METHODS
With Cox proportional hazards models we examined the relationship between initial T2DM remission and percent total weight loss (%TWL) after bariatric surgery. Categories of %TWL were included in the model as time-varying covariates.

RESULTS
Of patients (N = 5,928), 73% were female; mean age was 49.8 ± 10.3 years and BMI 43.8 ± 6.92 kg/m2, and 57% had Roux-en-Y gastric bypass (RYGB). Over an average follow-up of 5.9 years, 71% of patients experienced initial remission of T2DM (mean time to remission 1.0 year). With 0–5% TWL used as the reference group in Cox proportional hazards models, patients were more likely to remit with each 5% increase in TWL until 20% TWL (hazard ratio range 1.97–2.92). When categories &gt;25% TWL were examined, all patients had a likelihood of initial remission similar to that of 20–25% TWL. Patients who achieved &gt;20% TWL were more likely to achieve initial T2DM remission than patients with 0–5% TWL, even if they were using insulin at the time of surgery.

CONCLUSIONS
Weight loss after bariatric surgery is strongly associated with initial T2DM remission; however, above a threshold of 20% TWL, rates of initial T2DM remission did not increase substantially. Achieving this threshold is also associated with initial remission even in patients who traditionally experience lower rates of remission, such as patients taking insulin.
Knowledge, Attitude, and Practice of Mothers on the First 1000 Days of Life
Authors: Widyahening Indah Suci , Aufa Muhammad A , Alhas Muhammad F , Widodo Aji B , Friska Dewi 
Published: September 2021

The first 1000 days of life is the window period which critically determines the children’s growth and development and individual’s health afterwards. The knowledge, attitude, and practice (KAP) of pregnant mothers and mothers with children under two years old greatly influence the optimum upbringing of the fetus and children during the period. This study was aimed to evaluate the KAP of mothers about the first 1000 days of life and its associated factors. A cross-sectional study was conducted at a district in Depok city in August 2017. Subjects were purposively recruited to fill the validated KAP questionnaire on the first 1000 days. A total of 110 subjects were recruited, the higher proportion of them have adequate knowledge (42.7%), good attitude ((62.7%), and good practice (61.8%). The mothers’ knowledge was associated with attitude and practice (p<0.001) but the attitude and practice were not associated (p=0.175). Maternal education was the only factor that associated with knowledge (p=0.018) and attitude (p=0.013) while family income was associated with practice (p=0.018). This finding strengthened the importance of the existing community empowerment program such as posyandu in improving the KAP of mothers to achieve better results in the first 1000 days period of their children.
Keywords: the first 1000 days of life, knowledge, attitude, practice, nutrition.

Pengetahuan, Sikap dan Perilaku Ibu mengenai 1000 Hari Pertama
Abstrak
Seribu hari pertama kehidupan (1000 HPK) adalah periode emas yang sangat menentukan tumbuh kembang anak hingga kesehatan individu pada masa selanjutnya. Pengetahuan, sikap dan perilaku (PSP) ibu hamil dan ibu yang mengasuh anak berusia dua tahun ke bawah sangat menentukan asuhan 1000 HPK yang optimal. Penelitian ini bertujuan untuk mengetahui PSP ibu hamil dan ibu dengan anak berusia di bawah 2 tahun mengenai konsep 1000 HPK serta faktor – faktor yang berhubungan dengan ketiga aspek tersebut. Studi potong lintang dilakukan di Kampung Lio, Depok pada Agustus 2017. Ibu hamil dan ibu dengan anak berusia kurang dari 2 tahun direkrut secara purposif untuk mengisi kuesioner PSP tentang 1000 HPK. Sebanyak 110 subjek direkrut, proporsi terbesar subject memiliki tingkat pengetahuan cukup (42,7%), sikap sebagian besar baik (62,7%) dan perilaku sebagian besar juga baik (61,8%). Pengetahuan berhubungan dengan sikap dan perilaku (p<0.001) tetapi sikap dan perilaku tidak berhubungan (p=0,175). Tingkat pendidikan ibu adalah faktor yang berhubungan dengan pengetahuan (p=0,019) dan sikap (p=0,013). Tingkat penghasilan keluarga adalah faktor yang berhubungan dengan perilaku (p=0,018) mengenai 1000 HPK. Hasil ini menguatkan pentingnya posyandu sebagai upaya partisipasi masyarakat dalam meningkatkan PSP ibu untuk mencapai perkembangan anak yang optimal pada periode 1000 HPK.
Kata kunci: seribu hari pertama kehidupan, pengetahuan, sikap, perilaku, nutrisi.
European Cohesion Policy and Evaluating the Impact of Evidence-Based Policy
Authors: Rotar Laura Južnik , Trček Stanko 
Published: September 2021

Abstract
In the 2014–2020 programming period, the cohesion policy focuses more on results and evaluation of programs based on facts. Due to the Commission policies, an expansion of cohesion policy counterfactual impact evaluation of programmes with new approaches can be expected in the future. In this paper, the focus is on the calculation of the impact of received European cohesion funds on the revenue of companies in Slovenian municipalities one/two years after the receipt of cohesion funds for the 2007–2013 period. Two development priorities that affect company revenue – Enterprise competitiveness and research excellence and Promoting entrepreneurship and adaptability are considered. The effect of the use of the European cohesion funds on company revenue in Slovenian municipalities is positive for 2009 and 2010 and negative for all other years examined. The results of the research can serve to policy-makers to reduce the economic, social and territorial disparities in less developed European countries and regions therefore reaching balanced regional development.