bluuebunny/crossref_metadata_2025_split huggingface dataset:
QTLs for potato tuber resistance to Dickeya solani are located on chromosomes II and IV
Authors: Lebecka Renata , Śliwka Jadwiga , Grupa‐Urbańska Anna , Szajko Katarzyna , Marczewski Waldemar 
Published: May 2021

AbstractThe goal of this research was to identify quantitative trait loci (QTLs) for potato tuber resistance to the soil‐ and seedborne bacterium Dickeya solani and for tuber starch content, to study the relationship between these traits. A resistant diploid hybrid of potato, DG 00‐270, was crossed with a susceptible hybrid, DG 08‐305, to generate the F1 mapping population. Tubers that were wound‐inoculated with bacteria were evaluated for disease severity, expressed as the mean weight of rotted tubers, and disease incidence, measured as the proportion of rotten tubers. Diversity array technology (DArTseq) was used for genetic map construction and QTL analysis. The most prominent QTLs for disease severity and incidence were identified in overlapping regions on potato chromosome IV and explained 22.4% and 22.9% of the phenotypic variance, respectively. The second QTL for disease severity was mapped to chromosome II and explained 16.5% of the variance. QTLs for starch content were detected on chromosomes III, V, VI, VII, VIII, IX, XI, and XII in regions different from the QTLs for soft rot resistance. Two strong and reproducible QTLs for resistance to D. solani on potato chromosomes IV and II might be useful for further study of candidate genes and marker development in potato breeding programmes. The relationship between tuber resistance to bacteria and the starch content in potato tubers was not confirmed by QTL mapping, which makes the selection of genotypes highly resistant to soft rot with a desirable starch content feasible.
Does cognitive decline/dementia increase Delirium risk after stroke?
Authors: Ding Guanfu , Hua Shunde , Chen Jinming , Yang Shaochun , Xie Ruilu 
Published: May 2021

BackgroundDelirium is a common contributor to mortality and hospital costs in stroke patients. Different observational studies have showed inconsistent results regarding the association between cognitive decline/dementia and delirium after acute stroke. Therefore, we performed this meta‐analysis with the aim of determining whether cognitive decline/dementia is related to the risk of delirium after acute stroke.MethodsWe systematically searched PubMed, Embase, Google Scholar, and Web of Science for relevant studies from inception to September 2020. We calculated the pooled odds ratio (OR) with 95% confidence interval (CI) by using fixed or random effects models based on heterogeneity measured by theI2index.ResultsThe association between cognitive decline/dementia and post‐stroke delirium was examined in 13 studies with 3183 participants. After pooled analysis, we found that cognitive decline/dementia was significantly associated with susceptibility to delirium in post‐stroke patients (OR = 3.70, 95%CI: 2.90–4.71,P < 0.001). Subgroup analysis suggested that cognitive decline/dementia was associated with an increased risk of delirium in Caucasians (OR = 3.73, 95%CI: 2.73–5.11,P < 0.001), non‐Caucasians (OR = 3.65, 95%CI: 2.50–5.33,P < 0.001), samples with <200 subjects (OR = 3.70, 95%CI: 2.17–6.31,P < 0.001), samples with ≥200 subjects (OR = 3.70, 95%CI: 2.82–4.85,P < 0.001), studies published in 1990–2009 (OR = 3.17, 95%CI: 1.48–6.77,P = 0.003), and studies published in 2010–2020 (OR = 3.76, 95%CI: 2.92–4.86,P < 0.001).ConclusionsWe identified an association between cognitive decline/dementia and the risk of developing delirium. Cognitive decline/dementia may be a promising predictor for delirium in post‐stroke patients.
A privacy‐preserving framework for location recommendation using decentralized collaborative machine learning
Authors: Rao Jinmeng , Gao Song , Li Mingxiao , Huang Qunying 
Published: May 2021

AbstractThe nowadays ubiquitous location‐aware mobile devices have contributed to the rapid growth of individual‐level location data. Such data are usually collected by location‐based service platforms as training data to improve their predictive models' performance, but the collection of such data may raise public concerns about privacy issues. In this study, we introduce a privacy‐preserving location recommendation framework based on a decentralized collaborative machine learning approach: federated learning. Compared with traditional centralized learning frameworks, we keep users' data on their own devices and train the model locally so that their data remain private. The local model parameters are aggregated and updated through secure multiple‐party computation to achieve collaborative learning among users while preserving privacy. Our framework also integrates information about transportation infrastructure, place safety, and flow‐based spatial interaction to further improve recommendation accuracy. We further design two attack cases to examine the privacy protection effectiveness and robustness of the framework. The results show that our framework achieves a better balance on the privacy–utility trade‐off compared with traditional centralized learning methods. The results and ensuing discussion offer new insights into privacy‐preserving geospatial artificial intelligence and promote geoprivacy in location‐based services.
The Impact of Low Energy Availability on Nonexercise Activity Thermogenesis and Physical Activity Behavior in Recreationally Trained Adults
Authors: Martin Alexandra , Hofmann Hande , Drenowatz Clemens , Wallmann-Sperlich Birgit , Sperlich Billy , Koehler Karsten 
Published: May 2021

Energy availability describes the amount of dietary energy remaining for physiological functionality after the energy cost of exercise is deducted. The physiological and hormonal consequences of low energy availability (LEA) are well established, but the impact of LEA on physical activity behavior outside of exercise and, specifically, nonexercise activity thermogenesis (NEAT) has not been systematically examined. The authors conducted a secondary analysis of a repeated-measures crossover study in which recreationally trained young men (n = 6, 25 ± 1.0 years) underwent two 4-day conditions of LEA (15 kcal·kg fat-free mass−1 ·day−1) with and without endurance exercise (LEA + EX and LEA EX) and two energy-balanced control conditions (CON + EX and CON EX). The duration and intensity of physical activity outside of prescribed exercise were assessed using the SenseWear Pro3 armband. LEA did not alter NEAT (p = .41), nor time spent in moderate to vigorous (p = .20) and low-intensity physical activity (p = .17). However, time spent in low-intensity physical activity was lower in LEA + EX than LEA − EX (13.7 ± 0.3 vs. 15.2 ± 0.3 hr/day; p = .002). Short-term LEA does not seem to impact NEAT per se, but the way it is attained may impact physical activity behavior outside of exercise. As the participants expended similar amounts of energy during NEAT (900–1,300 kcal/day = 12.5–18.0 kcal·kg fat-free mass−1·day−1) and prescribed exercise bouts (15.0 kcal·kg fat-free mass−1·day−1), excluding it as a component of energy expenditure may skew the true energy available for physiological functionality in active populations.
Is a Head-Worn Inertial Sensor a Valid Tool to Monitor Swimming?
Authors: Shell Stephanie J. , Clark Brad , Broatch James R. , Slattery Katie , Halson Shona L. , Coutts Aaron J. 
Published: May 2021

Purpose: This study aimed to independently validate a wearable inertial sensor designed to monitor training and performance metrics in swimmers. Methods: A total of 4 male (21 [4] y, 1 national and 3 international) and 6 female (22 [3] y, 1 national and 5 international) swimmers completed 15 training sessions in an outdoor 50-m pool. Swimmers were fitted with a wearable device (TritonWear, 9-axis inertial measurement unit with triaxial accelerometer, gyroscope, and magnetometer), placed under the swim cap on top of the occipital protuberance. Video footage was captured for each session to establish criterion values. Absolute error, standardized effect, and Pearson correlation coefficient were used to determine the validity of the wearable device against video footage for total swim distance, total stroke count, mean stroke count, and mean velocity. A Fisher exact test was used to analyze the accuracy of stroke-type identification. Results: Total swim distance was underestimated by the device relative to video analysis. Absolute error was consistently higher for total and mean stroke count, and mean velocity, relative to video analysis. Across all sessions, the device incorrectly detected total time spent in backstroke, breaststroke, butterfly, and freestyle by 51% (15%). The device did not detect time spent in drill. Intraclass correlation coefficient results demonstrated excellent intrarater reliability between repeated measures across all swimming metrics. Conclusions: The wearable device investigated in this study does not accurately measure distance, stroke count, and velocity swimming metrics or detect stroke type. Its use as a training monitoring tool in swimming is limited.
Peritoneal or mesenteric tumours revealing histiocytosis
Authors: Cohen-Aubart Fleur , Ungureanu Irena , Razanamahery Jerome , Charlotte Frédéric , Valmary-Degano Séverine , Hélias-Rodzewicz Zofia , Cazals-Hatem Dominique , Dartigues Peggy , Delage-Corre Manuela , Selves Janick , Tas Patrick , Humbert Sebastien , Malakhia Alexandre , Kunnamo Merja , Veresezan Liana , Prokopiou Chrystalla , Seeber Andreas , Tazi Abdellatif , Donadieu Jean , Lucidarme Olivier , Haroche Julien , Emile Jean-François 
Published: May 2021

ObjectivePeritoneal or mesenteric tumours may correspond to several tumour types or tumour-like conditions, some of them being represented by histiocytosis. This rare condition often poses diagnostic difficulties that can lead to important time delay in targeted therapies. Our aim was to describe main features of histiocytoses with mesenteric localisation that can improve the diagnostic process.DesignWe performed a retrospective study on 22 patients, whose peritoneal/mesenteric biopsies were infiltrated by histiocytes.ResultsAbdominal pain was the revealing symptom in 10 cases, and 19 patients underwent surgical biopsies. The diagnosis of histiocytosis was proposed by initial pathologists in 41% of patients. The other initial diagnoses were inflammation (n=7), sclerosing mesenteritis (n=4) and liposarcoma (n=1). The CD163/CD68+CD1a- histiocytes infiltrated subserosa and/or deeper adipose tissues in 16 and 14 cases, respectively. ABRAFV600Emutation was detected within the biopsies in 11 cases, and two others were MAP2K1 mutated. The final diagnosis was histiocytosis in 18 patients, 15 of whom had Erdheim-Chester disease. The median diagnostic delay of histiocytosis was 9 months. Patients treated with BRAF or MEK inhibitors showed a partial response or a stable disease. One patient died soon after surgery, and five died by the progression of the disease.ConclusionDiagnosis of masses arising in the mesentery should be carefully explored as one of the possibilities in histiocytosis. This diagnosis is frequently missed on mesenteric biopsies. Molecular biology for detecting the mutations in BRAF or in genes of the MAP kinase pathway is a critical diagnostic tool.
Parental and professional perceptions of informed consent and participation in a time-critical neonatal trial: a mixed-methods study in India, Sri Lanka and Bangladesh
Authors: Pant Stuti , Elias Maya Annie , Woolfall Kerry , Morales Maria Moreno , Lincy Bensitta , Jahan Ismat , Sumanasena Samanmali P , Ramji Siddarth , Shankaran Seetha , Thayyil Sudhin 
Published: May 2021

IntroductionTime-critical neonatal trials in low-and-middle-income countries (LMICs) raise several ethical issues. Using a qualitative-dominant mixed-methods design, we explored informed consent process in Hypothermia for encephalopathy in low and middle-income countries (HELIX) trial conducted in India, Sri Lanka and Bangladesh.MethodsTerm infants with neonatal encephalopathy, aged less than 6 hours, were randomly allocated to cooling therapy or usual care, following informed parental consent. The consenting process was audio-video (A-V) recorded in all cases. We analysed A-V records of the consent process using a 5-point Likert scale on three parameters—empathy, information and autonomy. In addition, we used exploratory observation method to capture relevant aspects of consent process and discussions between parents and professionals. Finally, we conducted in-depth interviews with a subgroup of 20 parents and 15 healthcare professionals. A thematic analysis was performed on the observations of A-V records and on the interview transcripts.ResultsA total of 294 A-V records of the HELIX trial were analysed. Median (IQR) score for empathy, information and autonomy was 5 (0), 5 (1) and 5 (1), respectively. However, thematic analysis suggested that the consenting was a ceremonial process; and parental decision to participate was based on unreserved trust in the treating doctors, therapeutic misconception and access to an expensive treatment free of cost. Most parents did not understand the concept of a clinical trial nor the nature of the intervention. Professionals showed a strong bias towards cooling therapy and reported time constraints and explaining to multiple family members as key challenges.ConclusionDespite rigorous research governance and consent process, parental decisions were heavily influenced by situational incapacity and a trust in doctors to make the right decision on their behalf. Further research is required to identify culturally and context-appropriate strategies for informed trial participation.
Temporal trends and sex differences in sudden cardiac death in the Copenhagen City Heart Study
Authors: Ågesen Frederik Nybye , Lynge Thomas Hadberg , Blanche Paul , Banner Jytte , Prescott Eva , Jabbari Reza , Tfelt-Hansen Jacob 
Published: May 2021

ObjectiveMore knowledge about the development of sudden cardiac death (SCD) in the general population is needed to develop meaningful predictors of SCD. Our aim with this study was to estimate the incidence of SCD in the general population and examine the temporal changes, demographics and clinical characteristics.MethodsAll participants in the Copenhagen City Heart Study were followed from 1993 to 2016. All death certificates, autopsy reports and national registry data were used to identify all cases of SCD.ResultsA total of 14 562 subjects were included in this study. There were 8394 deaths with all information available, whereof 1335 were categorised as SCD. The incidence of SCD decreased during the study period by 41% for persons aged 40–90 years, and the standardised incidence rates decreased from 504 per 100 000 person-years (95% CI 447 to 569) to 237 per 100 000 person-years (95% CI 195 to 289). The incidence rate ratio of SCD between men and women ≤75 years was 1.99 (95% CI 1.62 to 2.46). The proportion of SCD of all cardiac deaths decreased during the observation period and decreased with increasing age. Men had more cardiovascular comorbidities (OR 1.34, 95% CI 1.07 to 1.68, p<0. 01), and SCD was the first registered manifestation of cardiac disease in 50% of all cases.ConclusionThe incidence of SCD in the general population has declined significantly during the study period but should be further investigated for more recent variations as well as novel risk predictors for persons with low to medium risk of SCD.
Serum high-molecular-weight adiponectin and response to dapagliflozin in patients with type 2 diabetes and non-alcoholic fatty liver disease
Authors: Aso Yoshimasa , Sagara Masaaki , Niitani Takafumi , Kato Kanako , Iijima Toshie , Tomaru Takuya , Jojima Teruo , Usui Isao 
Published: May 2021

A better baseline renal function is associated with a better response to sodium-glucose co-transporter-2 inhibitors in patients with type 2 diabetes. Low serum adiponectin is associated with visceral fat accumulation and hepatic steatosis. We investigated the relationship between baseline serum adiponectin and glycemic response to dapagliflozin in patients with type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). In a randomized, active-controlled, open-label trial, 57 patients with type 2 diabetes and NAFLD were randomized to either the dapagliflozin (5 mg/d) group or the control group. Both groups were treated for 24 weeks. Serum high-molecular-weight (HMW) adiponectin was measured with an ELISA kit. Visceral fat area (VFA) was measured by dual bioelectrical impedance analysis. Hepatic steatosis was assessed by the controlled attenuation parameter (CAP) measured by a transient elastography (FibroScan). Treatment with dapagliflozin significantly decreased HbA1c from 8.4%±1.5% at baseline to 7.4%±1.2% at 24 weeks. Both VFA and CAP decreased in the dapagliflozin group. Baseline serum HMW adiponectin was negatively correlated with changes in HbA1c from baseline to 24 weeks with dapagliflozin therapy. In the multivariate analysis, baseline HbA1c (β=−0.559, p=0.002) and serum HMW adiponectin (β=0.471, p=0.010) were independent determinants for the change (reduction) in HbA1c. In the dapagliflozin group, the change in HbA1c was positively correlated with the changes of CAP, but negatively correlated with the change in serum HMW adiponectin. In conclusion, a lower serum level of HMW adiponectin was associated with a better response to dapagliflozin in patients with type 2 diabetes and NAFLD. Trial registration number UMIN000022155.
Community-based aortic stenosis detection: clinical and echocardiographic screening during influenza vaccination
Authors: Steeds Richard Paul , Potter Andrew , Mangat Navjeet , Fröhlich Maren , Deutsch Cornelia , Bramlage Peter , Thoenes Martin 
Published: May 2021

BackgroundDegenerative aortic stenosis (AS), the most common valvular heart disease in the Western world, is often diagnosed late when the mortality risk becomes substantial. We determined the feasibility of AS screening during influenza vaccination at general practitioner (GP) surgeries in the UK.MethodsConsecutive subjects aged >65 years presenting to a GP for influenza vaccination underwent heart auscultation and 2D echocardiography (V-scan). Based on these findings, a patient management strategy was determined (referral to cardiologist, review within own practice or no follow-up measures) and status at 3 months was determined.Results167 patients were enrolled with a mean age of 75 years. On auscultation, a heart murmur was detected in 30 of 167 (18%) patients (6 subjects with an AS-specific and 24 with a non-specific murmur). 75.2% of those with no murmur had a negative V-scan finding. Conversely, 16 of 30 (53%) patients with any murmur had an abnormal V-scan finding that was largely related to the aortic valve. Using clinical auscultation and V-scan screening, a decision not to pursue follow-up measures was taken in 147 (88%) cases, whereas 18 (10.8%) subjects were referred onward; with 5 of 18 (27.8%) and 3 of 18 (16.7%) being diagnosed with mild and moderate AS.ConclusionsOur pilot study confirms feasibility of valvular heart disease screening in the elderly in a primary care setting. Using simple and inexpensive diagnostic measures and 7.3 million UK inhabitants undergoing influenza vaccination, nationwide screening could potentially identify 130 000 patients with moderate AS and a significant number of patients with severe AS.
Mismatch between midline shift and hematoma thickness as a prognostic factor of mortality in patients sustaining acute subdural hematoma
Authors: de Souza Matheus Rodrigues , Fagundes Caroline Ferreira , Solla Davi Jorge Fontoura , da Silva Gustavo Carlos Lucena , Barreto Rafaela Borin , Teixeira Manoel Jacobsen , Oliveira de Amorim Robson Luis , Kolias Angelos G , Godoy Daniel , Paiva Wellingson Silva 
Published: May 2021

BackgroundAcute subdural hematoma (ASDH) is a traumatic lesion commonly found secondary to traumatic brain injury. Radiological findings on CT, such as hematoma thickness (HT) and structures midline shift (MLS), have an important prognostic role in this disease. The relationship between HT and MLS has been rarely studied in the literature. Thus, this study aimed to assess the prognostic accuracy of the difference between MLS and HT for acute outcomes in patients with ASDH in a low-income to middle-income country.MethodsThis was a post-hoc analysis of a prospective cohort study conducted in a university-associated tertiary-level hospital in Brazil. The TRIPOD (Transparent Reporting of a multivariable prediction model for Individual Prognosis or Diagnosis) statement guidelines were followed. The difference values between MLS and HT (Zumkeller index, ZI) were divided into three categories (<0.00, 0.01–3, and >3). Logistic regression analyses were performed to reveal the OR of categorized ZI in predicting primary outcome measures. A Cox regression was also performed and the results were presented through HR. The discriminative ability of three multivariate models including clinical and radiological variables (ZI, Rotterdam score, and Helsinki score) was demonstrated.ResultsA total of 114 patients were included. Logistic regression demonstrated an OR value equal to 8.12 for the ZI >3 category (OR 8.12, 95% CI 1.16 to 40.01; p=0.01), which proved to be an independent predictor of mortality in the adjusted model for surgical intervention, age, and Glasgow Coma Scale (GCS) score. Cox regression analysis demonstrated that this category was associated with 14-day survival (HR 2.92, 95% CI 1.38 to 6.16; p=0.005). A multivariate analysis performed for three models including age and GCS with categorized ZI or Helsinki or Rotterdam score demonstrated area under the receiver operating characteristic curve values of 0.745, 0.767, and 0.808, respectively.ConclusionsThe present study highlights the potential usefulness of the difference between MLS and HT as a prognostic variable in patients with ASDH.Level of evidenceLevel III, epidemiological study.
Why a new volume on non-pollen palynomorphs?
Authors: O'Keefe Jennifer M. K. , Marret Fabienne , Osterloff Peter , Pound Matthew J. , Shumilovskikh Lyudmila 
Published: May 2021

Abstract

Here we introduce the volume
Applications of Non-Pollen Palynomorphs: from Palaeoenvironmental Reconstructions to Biostratigraphy
. The study of non-pollen palynomorphs (NPPs) has a long and rich history that is interwoven with that of pollen-based studies. NPPs are among the oldest fossils on record and are instrumental in determining the origin and evolution of life, as well as studying origination and extinction events prior to the origin of pollen-producing angiosperms. This new volume on NPPs provides an up-to-date and seminal overview of the subject, linking deep-time and Quaternary study of the subject for the first time.
Implications of Enhancer Transcription and eRNAs in Cancer
Authors: Adhikary Santanu , Roy Siddhartha , Chacon Jessica , Gadad Shrikanth S. , Das Chandrima 
Published: May 2021

Abstract
Despite extensive progress in developing anticancer therapies, therapy resistance remains a major challenge that promotes disease relapse. The changes that lead to therapy resistance can be intrinsically present or may be initiated during treatment. Genetic and epigenetic heterogeneity in tumors make it more challenging to deal with therapy resistance. Recent advances in genome-wide analyses have revealed that the deregulation of distal gene regulatory elements, such as enhancers, appears in several pathophysiological conditions, including cancer. Beyond the conventional function of enhancers in recruiting transcription factors to gene promoters, enhancer elements are also transcribed into noncoding RNAs known as enhancer RNAs (eRNA). Accumulating evidence suggests that uncontrolled enhancer activity with aberrant eRNA expression promotes oncogenesis. Interestingly, tissue-specific, transcribed eRNAs from active enhancers can serve as potential therapeutic targets or biomarkers in several cancer types. This review provides a comprehensive overview of the mechanisms of enhancer transcription and eRNAs as well as their potential roles in cancer and drug resistance.