bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Therapeutic Mechanism of LXGYD on Intestinal Stem Cells and Tight Junction Proteins in GI-ARS Rats
Authors: Yan Ziqiao , Yin Bofeng , Wang Yuguo , Feng Jian , Yang Qianyu , Li Xiao , Zhu Heng , Dou Yongqi 
Published: December 2021

Abstract
Background: Gastrointestinal acute radiation injury syndrome (GI-ARS) is potentially lethal and may occur after exposure to high radiation doses. Various chemical and biological agents have been developed to treat GI-ARS. However, their clinical utility is limited as they induce serious adverse reactions at their effective doses. Chinese herbal medicines have attracted attention because of their protective efficacy and low toxicity in radiation exposure treatment. However, their cellular and molecular mechanisms remain unknown. Here, we investigated the effects of the Chinese herbal Liangxue-Guyuan-Yishen decoction (LXGYD) on the intestinal stem cells and signal pathways of a GI-ARS rat model. Currently, there are limited treatment methods available globally; LXGYD might be a potential therapeutic option for patients with GI-ARS. Methods: The rat GI-ARS model was prepared by whole-body irradiation with 10-Gy of 60Co-γ rays. Various LXGYD concentrations were intragastrically administered to the irradiated rats. Health status and survival of the rats were evaluated and the protective efficacy of LXGYD on the intestines was assayed by pathological analysis. The active principles in LXGYD were detected by liquid chromatography-mass spectrometry (LC-MS) and their potential targets and pathways were screened by network pharmacological analysis. Intestinal stem cell proliferation, intestinal epithelial tight junction (TJ) protein expression, and regulatory pathways were explored by immunohistochemistry (IHC), western blotting (WB), and real-time quantitative polymerase chain reaction (RT-qPCR), respectively.Results: LXGYD administration significantly improved health status and survival in GI-ARS rats. The pathological analysis showed that LXGYD ameliorated radiation-induced intestinal injury. The LXGYD infusion significantly promoted LGR5+ stem cell regeneration in the ileal crypts, upregulated TJ proteins, and accelerated crypt reconstruction in the irradiated rats in a dose-dependent manner. LC-MS revealed ≥ 13 LXGYD constituents that might contribute to its protective effects. Involvement of the WNT and MEK/ERK pathways in intestinal repair and recovery were screened by network pharmacology analysis and validated by western blotting.Conclusions: The present study disclosed a heretofore unrecognized role of the Chinese herbal LXGYD in rescuing the intestinal stem cells of a GI-ARS rat model. It also showed that the WNT and MEK/ERK pathways may be involved in LXGYD-mediated intestinal regeneration in GI-ARS.
Comparison of the Clinical Characteristics and Prognosis Between Clear Cell Carcinomas and High-grade Serous Ovarian Carcinomas
Authors: Dong Shixiang , Yu Fengsheng , Liu Yutong , Yu Xiao , Sun Xin , Wang Wenjie , Wang Yankui 
Published: December 2021

Abstract
Objectives: To compare the clinical characteristics and prognosis of women with clear cell versus high-grade serous ovarian carcinoma.Methods: Retrospective analysis of the clinical data of 50 cases patients with ovarian clear cell carcinoma (OCCC) and 103 cases with high-grade serous ovarian carcinoma (HGSOC), who were initially treated and completed standardized therapy in Affiliated Hospital of Qingdao University from January 2013 to December 2017.Results: There were significant differences in age, gravidity (G > 1), chief complaint, with ovarian endometriosis, tumor diameter, unilateral or bilateral, cystic and solid tumor, CA125, HE4, CA199, lactate dehydrogenase (LDH), and FIGO stage between the two groups. The differences in the prognosis between OCCC patients and HGSOC patients with early stage (FIGO I-II) were not statistically significant. The 5-year overall survival and progression-free survival of OCCC patients were significantly worse than those of HGSOC patients with advanced stage (FIGO III-IV) (P < 0.05). FIGO stage and non-R0 resection were independent risk factors affecting the prognosis of patients with ovarian clear cell carcinoma, screening by Cox regression analysis. FIGO stage, the lowest value of CA125, and non-R0 resection were independent risk factors affecting the prognosis of patients with high-grade serous ovarian cancer.Conclusions: The clinical characteristics and prognosis of OCCC are different from those of HGSOC. OCCC patients have a significantly worse prognosis than those with HGSOC in the advanced stage (FIGO Ⅲ-Ⅳ). Satisfactory tumor resection is an essential factor related to the prognosis of patients with OCCC and HGSOC.
Experimental Study On Pyrolysis of Rice Straw Catalyzed By CaO/Al2O3-Phosphate Mixture
Authors: Xu Lianlian , Xu Zhongwen , Zhang Feng , Yuan Yinmei , Cheng Bin , Zhang Chuang , Chen Ping , Huang Xiangyong , Lin Yuyu , Gu Mingyan 
Published: December 2021

Abstract
This paper studied the synergistic effects of CaO or Al2O3 and three potassium phosphates (e.g., KH2PO4, K2HPO4·3H2O and K3PO4·3H2O) in the rice stalk pyrolysis through pyrolysis-gas chromatography-mass spectrometer (Py-GC/MS) experiments. The results show that after co-catalyzed by CaO/Al2O3 and potassium phosphates, the total contents of phenols, aldehydes, acids, LG from most samples decrease and those of ketones increase compared with those catalyzed by potassium phosphates alone. CaO/Al2O3 and potassium phosphates show synergistic effects in the regulation of the types or contents of phenols, ketones, aldehydes, etc. and are suitable for the production of ketone-rich bio-oil. Dehydration reactions, etc. are further promoted under the co-catalysis of the two catalysts, and some phenols can be converted to benzene products, etc. The contents of acetic acid can decrease to 0. For 50% K3PO4.3H2O impregnated sample, the yields of furans reduce sharply after CaO addition. For most impregnated samples except 50% K2HPO4·3H2O sample and 30%, 50% K3PO4.3H2O, the contents of total furans and furfural increase after Al2O3 addition.
Brain Magnetic Resonance Imaging Pattern in Cerebral Palsy
Authors: Gunawan Prastiya Indra , Noviandi Riza , Samosir Sunny Mariana 
Published: December 2021

Abstract
Background

Cerebral palsy (CP) leads to a common static motor neurological disease in children that can be demonstrated with varied neuroimaging findings. Magnetic Resonance Imaging (MRI) has a vital role of determining the presence of brain injury and its extent, with any possibility of determining pathogenic pattern and disease severity. The objective of the study is to evaluate the neuroimaging findings in CP and their correlation to disease severity.
Method

The research was case-control study, consecutive and complete records of all patients who had a clinical diagnosis of CP and performed a head MRI between 2018 and 2019 were enrolled in this study. Cases group were children diagnosed as severe CP with The Gross Motor Function Classification System (GMFCS) IV-V. Control group were children confirmed as CP with GMFCS I-III. Brain imaging was examined by MRI, in which the abnormalities were classified into grey matter or white matter injury, focal vascular disorder and brain malformation. Kruskal-Wallis statistical analysis was applied to identify the correlation.
Results

Almost 60 cases were reviewed. White matter injury, malformation and focal vascular insult were not correlated significantly to CP severity (OR = 0.73; 95% CI = 0.2-2.2; p = 0.78 and OR = 0.61; 95% CI = 0.2-1.9; p = 0.57 and OR = 2.034; 95% CI = 0.51-0.76; p = 0.63, respectively). Grey matter injury was more frequent discovered in severe CP (50%) and increased the risk of CP severity (OR = 9; 95% CI = 2.2 – 36; p = 0.002).
Conclusion

Grey matter injury is considered the most frequent abnormalities of Brain MRI in CP and it could increase the risk of severity.
New Physics II: Spin Picture, Particle Structure, and Fundamental Interactions
Authors: Kang China 
Published: December 2021

Abstract
Experimental data sometimes fails to render the expected truth, such as high-speed bullets smashing into pieces on a water surface cannot verify the water's hardness. By re-examining the essence underneath quantum phenomena and analyzing their relevance to universal classical theory, this study has thoroughly revealed the classical counterpart of spin. From now on, almost all quantum concepts (e.g., wave particle duality, quantum entanglement, and magnetic moment anomaly) can be understood classically, just as prominent physicists such as Planck, Einstein, and Schrödinger longed for back then.
Minimally Invasive Surgery in Emergency Surgery: A WSES Survey
Authors: Ceresoli Marco , Pisano Michele , Abu-Zidan Fikri , Allievi Niccolò , Gurusamy Kurinchi , Biffl Walt L. , Tebala Giovanni D. , Catena Fausto , Ansaloni Luca , Sartelli Massimo , Kluger Yoram , Baiocchi Gianluca , Group WSES MIS Working , Consortia WSES MIS , Coccolini Federico 
Published: December 2021

Abstract
Background: The diffusion of minimally invasive surgery in emergency surgery still represents a developing challenge. Evidence about the use of minimally invasive surgery shows its feasibility and safety, however the diffusion of these techniques is still poor. The aims of the present survey were to explore the diffusion and variations in the use of minimally invasive surgery among surgeons in the emergency setting.Methods: This is a web-based survey administered to all the WSES members investigating the diffusion of minimally invasive surgery in emergency. The survey investigated personal characteristics of participants, hospital characteristics, personal confidence in the use of minimally invasive surgery in emergency, limitations in the use of it and limitations to prosecute minimally invasive surgery in emergency surgery. Characteristics related to the use of minimally invasive surgery were studied with a multivariate ordinal regression.Results: The survey collected a total of 415 answers; 42.2% of participants declared a working experience >15 years and 69.4% of responders worked in tertiary level centre or academic hospital. In primary emergencies only28,7% of participants declared the use of laparoscopy in more than 50% of times. Personal confidence with minimally invasive techniques was the highest for appendectomy and cholecystectomy. At multivariate ordinal regression a longer professional experience, the use of laparoscopy in major elective surgery and bariatric surgery expertise were related to a higher use of laparoscopy in emergency surgery.Conclusions: The survey shows that minimally invasive techniques in emergency surgery are still underutilized. Greater focus should be placed on the development of dedicated training in laparoscopy among emergency surgeons.
Compressive Strength Evaluation of Concrete Confined With Spiral Stirrups by Using Adaptive Neuro-fuzzy Inference System (ANFIS)
Authors: Chang Wei , Zheng Wenzhong 
Published: December 2021

Abstract
The compressive strength of concrete confined with spiral stirrups was an important parameter to evaluate the load-bearing capacity of concrete columns. The confinement provided by spiral stirrups let concrete under the triaxial compression state and improved the compressive strength of concrete. However, the relationships between concrete and stirrups were complex and the existing prediction models for evaluating the compressive strength of confined concrete were various. In this paper, an adaptive neural-fazzy inferenxe system (ANFIS) model was developed to evaluate the compressive strength of concrete confined with stirrups. A set of 231 experimental results of concrete confined with spiral stirrups were collected from the previous studies to establish a reliable database. The investigated parameters included the aspect ratio of specimens, the diameter, spacing, yield strength, and volumetric ratio of stirrups, the ratio of longitudinal reinforcement, and the compressive strength of concrete. The results showed that the ANFIS model predicted the compressive strength of confined concrete accurately. By comparing with existing models, the proposed ANFIS model had high applicable and reliability. The effects of the investigated parameters on the compressive strength of concrete were analyzed based on the proposed ANFIS model.
Complete Genome Sequence of Bacteriophage EFC1 Infecting Enterococcus Faecalis From Chicken
Authors: Wang Qi , Liu Na 
Published: December 2021

Abstract
In response to Enterococcus faecalis infection of chicken origin, a multi host lytic phage, EFC1 was isolated and characterized the double-stranded circular DNA genome with size of 56099 bp, containing 89 predicted protein coding genes as well as 2 tRNAs involved in intron, structure, transcription, packaging, DNA replication, modification, lysis. Observation of the structure by electron microscopy and comparative phylogenetic analysis of terminase large subunit showed that the phage EFC1 belongs to a new member of Siphoviridae, which is relatively distantly related to its high similarity phages. The phage EFC1 has no relevant virulence genes and antibiotic resistance genes.
Prognostic Implication of Serum Glycated Albumin for Patients With Non-ST-segment Elevation Acute Coronary Syndrome Undergoing Percutaneous Coronary Intervention
Authors: Liu Chi , Zhao Qi , Ma Xiaoteng , Cheng Yujing , Sun Yan , Zhang Dai , Liu Xiaoli , Zhou Yujie 
Published: December 2021

Abstract
Background: It has been demonstrated that glycated albumin (GA) is significantly associated with diabetes complications and mortality. However, among patients diagnosed with non-ST-elevation acute coronary syndrome (NSTE-ACS) administered percutaneous coronary intervention (PCI), the predictive value of GA for poor prognosis is unclear.Methods: This study eventually included 2247 NSTE-ACS patients in Beijing Anzhen Hospital, Capital Medical University in January-December 2015 who received PCI. All patients were followed up until death or for 48 months post-discharge. The primary endpoint was major adverse cardio-cerebral events (MACCEs), including all-cause death, non-fatal myocardial infarction, ischemia-induced revascularization and non-fatal ischemic stroke.Results: In total, 547 (24.3%) MACCEs were recorded during the follow-up period. Upon adjusting for potential confounders, GA remained an important risk predictor of MACCEs (hazard ratio [HR]=1.051, 95% confidence interval [CI] 1.026-1.077; P<0.001). GA addition significantly enhanced the predictive ability of the traditional risk model (Harrell’s C-index, GA vs. Baseline model, 0.691 vs. 0.678, comparison P=0.001; continuous net reclassification improvement (continuous-NRI)=0.099, P=0.027; integrated discrimination improvement (IDI)=0.008, P=0.020).Conclusion: GA is highly correlated with poor prognosis in NSTE-ACS patients undergoing PCI, suggesting that it may be a major predictive factor of adverse events among these individuals.
The Incidence of Bordetella Pertussis Infections in Jahrom City, Southern Iran
Authors: Raufi Rahim , Shahriarirad Reza , Taghipour Nikta 
Published: December 2021

Abstract
Background: Bordetella Pertussis, known as the causative agent of whooping cough, is one of the leading causes of recurrent persistent cough at all ages, even in vaccinated individuals. Methods: A total number of 110 patients coughing for at least two weeks who were admitted to clinical centers in Jahrom, Iran, were included in this cross-sectional study. For this purpose, blood samples were collected from these individuals at two stages, i.e., at the beginning of the study and on the 21st day. Afterward, anti-pertussis toxin (PT) immunoglobulin G (IgG) was measured in serum samples via the enzyme-linked immunosorbent assay (ELISA). The given cases were further evaluated in terms of age, gender, occupation, place of living, and family size.Results: Out of 110 patients recruited in this study, 77 cases were female (70%). Also, only seven patients were shown to be serologically positive (6.4%). Moreover, no significant association was observed between pertussis incidence rate and the study variables, namely, age, gender, occupation, residency area (urban or rural), and family size (p>0.05).Conclusions: This study aimed to emphasize pertussis occurrence in individuals who merely present persistent cough without typical symptoms. This requires the physicians to conduct more precise assessments along with more rapid diagnostic methods.
Effect of timing of school enrollment on physical fitness in third graders
Authors: Fühner Thea , Granacher Urs , Golle Kathleen , Kliegl Reinhold 
Published: December 2021

Abstract
Timing of school enrollment may vary considerably due to children who were enrolled early / delayed or skipped / repeated a school year. Accordingly, the age range within school grades includes older-than-keyage (OTK) and younger-than-keyage (YTK) children. Studies examining academic performance of OTK and YTK children versus keyage children reported large differences in academic performance. However, there are no studies available that examined physical fitness of OTK and YTK versus keyage children. The aim of this study was to compare physical fitness of OTK (N = 25,086) and YTK (N = 1,885) children versus keyage children (N = 108,295) in a sample of German third graders. Physical fitness tests comprised cardiorespiratory endurance, coordination, speed, lower, and upper limbs muscle power. Predictions of physical fitness performance for YTK and OTK children were estimated using data from keyage children by taking age, sex, school, and cohort into account. The difference between observed and predicted z-scores yielded a delta z-score that was used as a dependent variable in the linear mixed models. Findings indicate that OTK children showed impaired performance compared to keyage children, especially in coordination, and that YTK children outperformed keyage children, especially in coordination. Teachers should be aware that despite their age advantage, OTK children often show impaired fitness compared to keyage children.
Intravitreal Injections and Face Masks: Endophthalmitis Risk Before and During the COVID-19 Pandemic
Authors: Karimi Saeed , Mohammadzadeh Amir , Ramezani Alireza , Ansari Iman , Nouri Hosein , Abtahi Seyed-Hossein , Nikkhah Homayoun 
Published: December 2021

Abstract
In this retrospective, single-center study, the records of patients with acute endophthalmitis following intravitreal bevacizumab (IVB) injections during the pre-COVID and COVID-19 eras were reviewed and compared. A total of 28,085 IVB injections were performed during the pre-COVID era; nine eyes of nine patients developed acute post-IVB endophthalmitis in this era, giving an overall incidence of 0.032% (3.2 in 10000 injections). In the COVID era, a total of 10,717 IVB injections were performed; four eyes of four patients developed acute post IVB endophthalmitis in this era, giving an overall incidence of 0.037% (3.7 in 10000 injections). The incidences of post-IVB endophthalmitis during these two eras were not different (P-value = 0.779). The BCVA of the eyes affected during the COVID era showed poor improvement back to baseline values, after three months, even with prompt and maximum treatment. Face masking protocols seem unlikely to increase the risk of post-IVB endophthalmitis.
A Covariant Cauchy-Schwartz Measure’s Bounding Conditions to BSM Searches
Authors: Maalouf Hani W. 
Published: December 2021

Abstract
Solving for the missing masses in the Higgs resonances, it was necessary to extend, even quantitatively via an index measurable amount, the SM using a threshold related longitudinal violation procedure. The obtained expression, by being non-contributing via its non-anomalously resulting parameter, is linked to a Cauchy-Schwartz 4-scalar product ratio type of two virtual Gauge Bosons momenta in its minimal anomalous configuration, as vs. its non-anomalous internal. Changing the bounds from energy into momenta, a convexity condition appears. Such technique clarifies the perturbative e.m. fields’ extensions into perturbative and non-perturbative QCD.In applications, there is the violation of the chiral insertion by the axion into neutrinos, and the Lepton number when passing form velocity to spin resonances, such confirming the CS procedure as plus the defiance of the SM comes through their branching ratios but not their angular distributions. Further which if remaining at the same level of minimization can restore the universality of extendibility in the Higgs self-couplings.Leading into deriving the phase of K0 → π+π-, in A(∆1=2)/A(∆1=0) so a conformal skipping dynamical shift from direct CP violation of D0 → K+K- and D0 → π+π- asymmetries, in the long-short mixing concords the phase of KL → π0ννbar, solving the KOTO anomaly.
Bursatella leachii purple ink secretion extract exerts cytotoxic properties against human hepatocarcinoma cell line (HepG2): In vitro and in silico studies
Authors: Alehaideb Zeyad ,   Mahadev Kokane2,   Anuradha Venkatraman2, Mohamed Syed Ali , Rameshbabu Saranya , Suliman Rasha S. , Alghamdi Sahar S. , Al-Eidi Hamad , Alghanem Bandar , Abdulla Maha-Hamadien , Matou-Nasri Sabine 
Published: December 2021

Abstract

Liver cancer is the third leading cause of cancer death worldwide. Marine mollusc-derived extracts have gained attention as new potential natural-based anticancer agents to overcome the side effects caused by conventional chemotherapeutic drugs during cancer therapy. We evaluated the cytotoxic effects of a crude extract from the purple-ink released by the sea hare named Bursatella leachii (B. leachii) against human hepatocarcinoma cell line (HepG2) and explored the underlying mechanisms causing the programmed cell death (i.e., apoptosis). Expression of cleaved-caspase-8 and cleaved-caspase-3, key cysteine-aspartic proteases involved in the initiation and completion of the apoptosis process, appeared after HepG2 cell exposure to B. leachii extract. Gene expression levels of pro-apoptotic BAX, tumour suppressor TP53 and Cyclin D1 were increased after treatment with B. leachii. Using liquid chromatography-mass spectrometry, the main biomolecules in the B. leachii extract were identified as hectochlorin, malyngamide X, malyngamide S, bursatellin, and lyngbyatoxin A. Applying in silico approaches, the high scores predicted bioactivities for the five compounds were protease and kinase inhibitors. The ADME and cytochrome profiles for the compounds were also predicted. Altogether, the cytotoxic B. leachii extract presents high pro-apoptotic potentials, suggesting it as a promising safe natural product-based drug for the treatment of liver cancer.