bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuron Navigator 3 (NAV3) is Required for Heart Development in Zebrafish
Authors: Lv Feng , Ge Xiaojuan , Qian Peipei , Lu Xiaofeng , Liu Dong , Chen Changsheng 
Published: December 2021

Abstract
As a tightly controlled biological process, cardiogenesis requires the specification and migration of a suite of cell types to form a particular three-dimensional configuration of the heart. Many genetic factors are involved in the formation and maturation of the heart, and any genetic mutations may result in severe cardiac failures. The neuron navigator (NAV) family consists of three vertebrate homologs (NAV1, NAV2, and NAV3) of the neural guidance molecule Uncoordinated-53 (UNC-53) in Caenorhabditis elegans. Although they are recognized as neural regulators, their expressions are also detected in many organs, including the heart, kidney, and liver. However, the functions of NAVs, regardless of neural guidance, remain largely unexplored. In our study, we found that nav3 gene was expressed in the cardiac region of zebrafish embryos from 24 to 48 hours post-fertilization (hpf) by means of in situ hybridization (ISH) assay. A CRISPR/Cas9-based genome editing method was utilized to delete the nav3 gene in zebrafish and loss-of-function of Nav3 resulted in a severe deficiency in its cardiac morphology and structure. The similar phenotypic defects of the knockout mutants could recur by nav3 morpholino injection and be rescued by nav3 mRNA injection. Dual-color fluorescence imaging of ventricle and atrium markers further confirmed the disruption of the heart development in nav3-deleted mutants. Although the heart rate was not affected by the deletion of nav3, the heartbeat intensity was decreased in the mutants. All these findings indicate that Nav3 was required for cardiogenesis in developing zebrafish embryos.
Process Simulation Integrated Life Cycle Net Energy Analysis and GHG Assessment of Fuel-Grade Bioethanol Production from Unutilized Rice Straw
Authors: Jayasundara Piyumali Mewanthika , Jayasinghe Thisara Kaveendra , Rathnayake Mahinsasa 
Published: December 2021

Abstract
The life cycle stage of paddy rice cultivation can be excluded with a zero-inventory allocation rule for the life cycle scenario of bioethanol production from unutilized rice straw, i.e., rice straw with no applied valorization in current practice. Accordingly, this study evaluates the life cycle net energy analysis and greenhouse gas (GHG) assessment for a scaled-up bioethanol production plant using unutilized rice straw as the feedstock. The process simulation technique is integrated to model a scaled-up production plant to produce bioethanol at 99.7 vol% purity from unutilized rice straw, and the simulation results are retrieved to calculate inventory data for life cycle assessment (LCA). The simulated mass flow and energy flow results are comparable with that of real plants, reported in the published literature, which validates the process simulations in this study. Inclusive of energy generation using the waste flows in the process (i.e., wastewater and solid residues), the life cycle net energy analysis results show a net energy gain of 7,804.0 MJ/m3 of bioethanol with a net renewable energy gain of 38,230.9 MJ/m3 of bioethanol that corresponds to a net energy ratio of 1.20 and renewability factor of 5.49. The life cycle GHG assessment exhibits a net global warming potential of 584.8 kg CO2 eq./m3 of bioethanol. The effect of system boundary expansion up to the end-of-life stage as gasohol (E10), the sensitivity of the key process parameters, and the economic benefit via valorization of unutilized rice straw are further analyzed and discussed.
Complete Mitochondrial Genomes of Three Nuthatches From the Genus Sitta (Aves: Passeriformes: Sittidae) and Mitogenome-Based Phylogenetic Analysis
Authors: Yuan Qing-Miao , Luo Xu , Cao Jing , Duan Yu-Bao 
Published: December 2021

Abstract
Background

Nuthatches (genus Sitta) comprise a group of Passeriformes. With the publication of more mitochondrial genome data, there has been considerable focus on the taxonomic status of the nuthatches. To understand the phylogenetic position of Sitta and phylogenetic relations within this genus, we sequenced and analyzed the complete mitochondrial genomes of three species, S. himalayensis, S. nagaensis and S. yunnanensis, making this the first account of complete mitochondrial genomes (mitogenomes) for this genus.
Results

The mitochondrial genomes of three Sitta species are 16,822-16,830 bp in length and consisted of 37 genes and a control region. This study recovered the same gene arrangement found in the mitogenomes of Gallus gallus, which is considered the typical ancestral avian gene order. All tRNAs were predicted to form the typical cloverleaf secondary structures. Bayesian inference and maximum likelihood phylogenetic analyses of sequences of 18 species obtained a well-supported topology. The family Sittidae is the sister-group of Troglodytidae, and the genus Sitta can be divided into 3 major clades. We demonstrated the phylogenetic relationships within genus Sitta (S. carolinensis + (S. villosa + S. yunnanensis + (S. himalayensis + (S. europaea + S. nagaensis)))).
Bioethanol Production From Hassawi Rice Straw Wastes Assisted by Aspergillus sp. NAS51 Cellulosic Enzyme Under SSF and Saccharification Process With in Silico Enzyme Structural Homology Modeling
Authors: Shalabi Ahmed Ahmed Abdelghani Hamed Abdalla , Ibrahim Hala A. , Khedr Mohamed , Badawy Mona Shaban E. M. , Alamri Saad , Alfaifi Mohammad Y. , Shati Ali A. , Elbehairi Serag Eldin I. , Abd-Rabbo Hisham S.M. , Fawy Khalid F. , Hamdy Mohamed S. , Awwad Nasser S. 
Published: December 2021

Abstract
With the distribution of exploitable non-renewable energy resources, the use of lignocellulosic wastes to make bioethanol and biogas has drawn great attention from researchers. In our effort to find a potent cellulase-producing fungal strain, the fungus NAS51 was isolated from a sponge collected from the Red Sea, Jeddah, among eight isolates and selected as it displayed potent cellulolytic activity. The fungus was identified morphologically and genetically by sequencing its 18SrRNA gene as Aspergillus sp. NAS51. The cellulase activity of Aspergillus sp. NAS51 was optimized and maximum enzyme production was obtained at initial pH7, temp 30oC, incubation period 11 days, moisture content 70%, urea as a nitrogen source, and K2HPO4 (2g/L). The cellulase gene has been sequenced and the protein 3D structure was generated via in silico homology modeling. Determination of binding sites and biological annotations of the constructed protein was carried out via COACH and COFACTOR based on the I-TASSER structure prediction. To reach the maximum enzyme hydrolysis, the rice straw collected from Al-Ahsa, Kingdom of Saudi Arabia was pretreated with NaOH 1.5% to remove lignin and to enhance the saccharification process by cellulase enzyme. The saccharified product was measured using HPLC, fermented by S. cerevisiae and the bioethanol yield produced from the fermentation was 0.454 ml ethanol/g fermentable sugars.
Panel Data Analysis of Energy Conservation and Emission Reduction On High-Quality Development of Logistics Industry in Yangtze River Delta of China
Authors: Fan Linbang , Liu Hui , Shao Zhaoxia , Li Cunfang 
Published: December 2021

Abstract
With the implementation of China's carbon neutrality policy, the Yangtze River Delta region, as a benchmark for the development of China's logistics industry, calls for great concern, accompanying the energy consumption and environmental problems. In order to explore how China's logistics industry can achieve both energy conservation and emission reduction (ECER) and high-quality development in the context of carbon neutrality, this study analyzes the relationship between the development of logistics industry and the economy, energy together with environment in the Yangtze River Delta region based on the data of China Statistical Yearbook from 2001 to 2019, by means of the Entropy method and PVAR model. The main findings are summarized as follows: firstly, the economy, industrial structure, energy and environment all have significant impact on the development of logistics industry in Yangtze River Delta region. Secondly, the development of logistics industry in Yangtze River Delta region is not balanced, and the provinces including Jiangsu, Shanghai, Zhejiang and Anhui have great differences in economy, industrial structure, demographic dividend, energy consumption and environmental protection, and the possibility of complementary advantages. Thirdly, the economic development and energy consumption are two-way effects. Environmental protection is relevant to economic development, industrial structure, energy consumption and logistics supply. Finally, in the context of carbon neutrality, this paper gives some suggestions on how to realize the high-quality development of logistics industry in Yangtze River Delta region while considering energy consumption and environmental protection.
Immune Checkpoint Inhibitor Monotherapy is Associated With Less Cardiac Toxicity Than Combination Therapy
Authors: Cone Eugene B. , Haeuser Lorine , Reese Stephen , Marchese Maya , Nguyen David-Dan , Nabi Junaid , Chou Wesley H. , Noldus Joachim , McKay Rana R. , Kilbridge Kerry Laing , Trinh Quoc-Dien 
Published: December 2021

Abstract
Background: Treatment options for many cancers include immune checkpoint inhibitor (ICI) monotherapy and combination therapy with impressive clinical benefit across cancers. We sought to define the comparative cardiac risks of ICI combination and monotherapy.Methods: We used VigiBase, the World Health Organization pharmacovigilance database, to identify cardiac ADRs (cADRs), such as carditis, heart failure, arrhythmia, myocardial infarction, and valvular dysfunction, related to ICI therapy. To explore possible relationships, we used the reporting odds ratio (ROR) as a proxy of relative risk. A lower bound of a 95% confidence interval of ROR > 1 reflects a disproportionality signal that more ADRs are observed than expected due to chance.Results: We found 2278 cADR for ICI monotherapy and 353 for ICI combination therapy. Combination therapy was associated with significantly higher odds of carditis(ROR 6.9, 95% CI: 5.6–8.3) versus ICI monotherapy (ROR 5.0, 95% CI: 4.6-5.4).Carditis in ICI combination therapy was fatal in 23.4% of reported ADRs, compared to15.8% for ICI monotherapy (P = 0.058).Conclusions: Using validated pharmacovigilance methodology, we found increased odds of carditis for all ICI therapies, with the highest odds for combination therapy.Given the substantial risk of severe ADR and death, clinicians should consider these findings when prescribing checkpoint inhibitors.
Investigation into the Protective Effects of Hypaconitine and Glycyrrhetinic Acid Against Chronic Heart Failure of the Rats
Authors: Wang Liqin , Deng Haiming , Wang Tengyu , Qiao Yun , Zhu Jianbing , Xiong Mingfeng 
Published: December 2021

Abstract
BackgroundThe present study aimed to determine the protective effects of hypaconitine (HA) and glycyrrhetinic acid (GA) against chronic heart failure (CHF) in the rats and to explore the underlying molecular mechanisms.Methods The CHF rat model was established by transverse-aortic constriction (TAC) operation. The total cholesterol (TCHO) and triglyceride (TG) levels were determined by ELISA assay. The protein expression of fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) in the rat ventricular tissues was determined by immunohistochemistry. The serum metabolites were determined by LC-MS/MS assay.ResultsHA + GA treatment significantly reduced the plasma levels of TCHO and TG in the CHF rats. The expression of FGF2 and VEGFA protein was up-regulated and the expression of eNOS protein was down-regulated in the ventricular tissues of CHF rats, which was significantly restored after HA + GA treatment. HA + GA treatment down-regulated serum isonicotinic acid, phosphatidylcholine, cardiolipin, estrogen glucuronide, and glycocholic acid, up-regulated serum sphingosine and deoxycholic acid in the CHF rats.ConclusionIn conclusion, HA +GA showed protective effects on CHF in the rats, and the HA + GA may exert protective effects by reducing lipid levels, up-regulating the expression of FGF2 and VEGFA proteins, attenuating eNOS protein expression, and modulating metabolic pathways. However, the molecular mechanisms underlying HA + GA-mediated effects still require further examination.
Anthropogenic Climate Change Drives the Melting of Glaciers in the Himalaya
Authors: Romshoo Shakil A , Murtaza Khalid Omar , Shah Waheed , Ramzan Tawseef , Ameen Ummer , Bhat Mustafa Hameed 
Published: December 2021

Abstract
The Himalayan glaciers supply water to a large population in south Asia for various uses and ecosystem services. Therefore, regional monitoring of glacier melting and identifying the drivers thereof is important to understand and predict the future trends of cryospheric melting. Using multi-date satellite images from 2000-2020, we investigated the shrinkage, snout retreat, thickness changes, mass loss and velocity changes of 77 glaciers in the Drass basin, western Himalaya, India. The overall glacier cover has shrunk by 5.31±0.33 km2 during the period. Snout retreat varied between 30-430 m (mean 155±9.58 m). Debris-cover showed a significant influence on the glacier melting with the clean glaciers showing a higher loss of ~5% compared to the debris-covered glaciers (~2%). The glaciers on an average have shown thickness change and mass loss of -1.27±0.37 and -1.08±0.31 m w.e.a-1 respectively. Average glacier velocity has reduced from 21.35±3.3 m a-1 in 2000 to 16.68±1.9 m a-1 by 2020 due to the continuous melting and the consequent mass loss of the glaciers. Concentration of the greenhouse gases (GHGs), black carbon and other pollutants from vehicular traffic plying in the vicinity of the glaciers has significantly increased during the observation period. Increasing temperatures, result of the significant increase of the GHGs and pollutants in the atmosphere, drive the glacier melting in the study area. If the situation continues in the future, the glaciers may disappear altogether in the Himalaya leading to significant impact on the regional water supplies, hydrological processes, ecosystem services and transboundary sharing of waters.
Impact of Sectoral Decompositions of Electricity Consumption On Economic Growth In India: Evidence From SVAR Framework
Authors: Bal Debi Prasad , Patra Sujit Kumar , Mohanty Seba 
Published: December 2021

Abstract
This study examine the effects of electricity consumption from different sectors such as agricultural, commercial, domestic, Industrial (HV), Industrial (LV-MV) and Miscellaneous sector on economic growth over the period of 1981-2019 in case of India. We used SVAR framework and concluded that the consumption of electricity from agriculture sector has a negative impact on economic growth. Whereas, the Industrial (HV and MV-LV) and commercial electricity consumption have positive impact on economic growth. Similarly, electricity consumption by the domestic sector has less positive effect on economic growth. Further, we computed the total factor productivity growth (TFP) by using DEA method and show the effects of sector wise electricity consumption on TFP as the robustness of our analysis. We obtain similar kind of results. From the policy perceptive, the study suggests that government must speed up the construction of a power grid to improve the availability of electricity for achieving higher rate of economic growth.
TaLBD1, a LOB Transcription Factor Gene In T. Aestivum, Improves Plant N Starvation Adaptation Via Modulating N Acquisition-Associated Processes
Authors: Zhang Yanyang , Ni Chenyang , Li Tianjiao , Han Le , Du Pingping , Xiao Kai 
Published: December 2021

Abstract
Members of transcription factor (TF) families contribute largely to plant N starvation tolerance by regulating downstream stress defensive genes. In this study, we characterized TaLBD1, a Lateral Organ Boundary (LOB) TF gene in T. aestivum, in regulating plant low-N stress adaptation. TaLBD1 harbors the conserved domains specified by plant LOB proteins, targeting onto nucleus after endoplasmic reticulum (ER) assortment. The TaLBD1 transcripts were response sensitively to N starvation (NS) signaling, showing to be gradually upregulated in aerial and root tissues over a 27-h NS condition. The N. tabacum lines overexpressing TaLBD1 improved phenotype, root system architecture (RSA) establishment, biomass, and N contents of plants under NS treatment. The nitrate transporter gene NtNRT2.4 and PIN-FORMED gene NtPIN6 significantly upregulated in expression in NS-challenged lines; knockdown expression of NtNRT2.4 decreased N uptake and that of NtPIN6 alleviated RSA establishment relative to WT. These results validate the function of NRT and PIN genes in regulating plant N uptake and RSA behavior. RNA-seq analyses revealed that a quantity of genes modify expression in N-deprived lines overexpressing TaLBD1, which enriched into functional groups of signal transduction, transcription, protein biosynthesis, primary or secondary metabolism, and stress defensiveness. These findings suggested that the TaLBD1-improved NS adaptation attributes largely to its role in transcriptionally regulating NRT and PIN genes as well as in modulating those functional in various biological processes. TaLBD1 is a crucial regulator in plant N starvation tolerance and valuable target for molecular breeding high N use efficiency (NUE) crop cultivars.
Differential Fatty Acids and Stress Biomarkers Responses in Native (Carcinus Aestuarii) and Invasive (Portunus Segnis) Crabs Exposed to Environmental Pollution
Authors: BEJAOUI safa , CHETOUI Imene , GHRIBI Feriel , FOUZAI Chaima , TRABELSI Wafa , ZERELI Sonia , CAFSI Mhamed EL , SOUDANI Nejla 
Published: December 2021

Abstract
The present work aims to determine and compare the tolerance to different stress environmental conditions in two crabs’ species the native Carcinus aestuarii and the invasive Portunus segnis. In this study, C.aestuarii appeared more intolerant to environmental stressor as shown by the important changes occurred on the proximate, fatty acids and their essential compounds comparing to P.segnis. Our results revealed accumulation of metallic trace elements, macromolecular damages and antioxidant defense systems perturbations mostly in C.aesuarii gills and muscles than P.segnis, thus highlighting the importance of a multi-markers approach to assess the urban pollution in coastal ecosystems. According to this study, fatty acid profiles and oxidative stress biomarker examination of both crabs indicate that the different environmental stresses significantly influence the tolerance of C.aestuarii compared to the invasive P.segnis which may provide physiological advantages for the achievement of their invasion in novel ecosystems.
WITHDRAWN: Yoga Versus Physical Therapy In Multiple Sclerosis: Randomized Controlled Trial
Authors: Lysogorskaia Elena , Ivanov Timur , Ulmasbaeva Elena , Mendalieva Aynagul , Youshko Maxim , Brylev Lev 
Published: December 2021

Abstract
Background. Yoga originated in the territory of modern India more than 3000 years ago and uses techniques for working with the musculoskeletal system, cardiorespiratory system and attention. Currently, the effectiveness and safety of yoga in patients with various neurological disorders, including MS, is of interest to many scientists and clinicians. The main aim of this study is to examine the effect of yoga on symptoms and quality of life in patients with MS versus physical therapy (exercise therapy) and no exercise.Methods. The patients were randomly assigned to 3 groups (yoga, PT, or waiting list), patients from the waiting list had an opportunity to enter the yoga program after the end of the trial period. After 12 weeks of regular exercises (or absence of them), the effect of yoga and PT on the functional status and quality of life of patients were evaluated. The MS treatment was a part of routine practice, as prescribed by the treating neurologist. The data was collected during the patients’ two visits to the study center - before the start of the study and at the end of the 12-week period. The in-person examination included a doctor's assessment of the EDSS, the SF-36 quality of life questionnaire, the fatigue scale, the Berg balance scale, the 6-minute walking test.Results. A total of 36 patients finished the clinical study: 30 women and 6 men. There was no statistically significant difference between the groups in terms of improvement in MS symptoms as measured by the balance, walking test and fatigue scales. However, in the analysis of the 8 criteria of SF-36 quality-of-life questionnaire the covariation analysis statistically significant differences were found in favor of the yoga group in terms of physical functioning (PF) (p=0.003), life activity (VT) (p<0.001), mental health (MH) (p=013), social functioning (SF) (p=0.028). Conclusions. Thus, regular yoga classes under the guidance of qualified staff are a promising method of non-drug rehabilitation of patients with MS with motor disorders. More research is needed to examine the impact of yoga on clinical patient improvement and quality of life indicators.Trial registration. ISRCTN15486200 https://doi.org/10.1186/ISRCTN15486200
Association between Insomnia and Subclinical Atherosclerosis Among Chinese Steelworkers: A Cross-sectional Survey
Authors: Wang Lihua , Zhang Shengkui , Yu Miao , Yuan Juxiang 
Published: December 2021

Abstract
Background: Insomnia is a common prevalent sleep disorder. Difficulty maintaining sleep or poor in quality in insomnia caused by disrupted or misaligned circadian rhythms may play an important role in the development of atherosclerosis. This study aimed to examine the association between insomnia and subclinical atherosclerosis in Chinese steelworkers.Methods: A total of 3582 subjects from a large enterprise located in northern China were included in this study. The Athens Insomnia Scale (AIS) was used to assess the status of insomnia. Subclinical atherosclerosis was evaluated using ultrasonographic measurements of carotid plaque. Multivariable logistic regression was used to identify association between insomnia and carotid atherosclerosis.Results: The overall prevalence of insomnia and carotid plaque were 35.1% and 29.9% in the study population. Compared with non-insomnia workers, significantly increased odds of carotid plaque were observed among insomnia workers after adjustment for potential confounders, odds ratio (OR) = 1.38, 95% confidence interval (CI): 1.17 to 1.62. Exposure to current shift work and insomnia simultaneously can significantly elevated odds of carotid plaque. No significant association was found in female workers between insomnia and carotid plaque.Conclusion: Insomnia is associated with elevated odds of carotid atherosclerosis in male steelworkers. Insomnia problems of workers should receive further attention in occupational worker health interventions.