bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mammalian SWI/SNF chromatin remodeler is essential for reductional meiosis in males
Authors: Menon Debashish U. , Kirsanov Oleksandr , Geyer Christopher B. , Magnuson Terry 
Published: November 2021

AbstractThe mammalian SWI/SNF nucleosome remodeler is essential for spermatogenesis. Here, we identify a role for ARID2, a PBAF (Polybromo - Brg1 Associated Factor)-specific subunit, in meiotic division. Arid2cKO spermatocytes arrest at metaphase-I and are deficient in spindle assembly, kinetochore-associated Polo-like kinase1 (PLK1), and centromeric targeting of Histone H3 threonine3 phosphorylation (H3T3P) and Histone H2A threonine120 phosphorylation (H2AT120P). By determining ARID2 and BRG1 genomic associations, we show that PBAF localizes to centromeres and promoters of genes known to govern spindle assembly and nuclear division in spermatocytes. Consistent with gene ontology of target genes, we also identify a role for ARID2 in centrosome stability. Additionally, misexpression of genes such as Aurkc and Ppp1cc (Pp1γ), known to govern chromosome segregation, potentially compromises the function of the chromosome passenger complex (CPC) and deposition of H3T3P, respectively. Our data support a model where-in PBAF activates genes essential for meiotic cell division.
Substantially enhanced plasticity of bulk metallic glasses by densifying local atomic packing
Authors: Wu Yuan , Cao Di , Yao Yilin , Zhang Guosheng , Wang Jinyue , Liu Leqing , Li Fengshou , Fan Huiyang , Liu Xiongjun , Wang Hui , Wang Xianzhen , Zhu Huihui , Jiang Suihe , Kontis Paraskevas , Raabe Dierk , Gault Baptiste , Lu Zhaoping 
Published: November 2021

AbstractIntroducing regions of looser atomic packing in bulk metallic glasses (BMGs) was reported to facilitate plastic deformation, rendering BMGs more ductile at room temperature. Here, we present a different alloy design approach, namely, doping the nonmetallic elements to form densely packed motifs. The enhanced structural fluctuations in Ti-, Zr- and Cu-based BMG systems leads to improved strength and renders these solutes’ atomic neighborhoods more prone to plastic deformation at an increased critical stress. As a result, we simultaneously increased the compressive plasticity (from ∼8% to unfractured), strength (from ∼1725 to 1925 MPa) and toughness (from 87 ± 10 to 165 ± 15 MPa√m), as exemplarily demonstrated for the Zr20Cu20Hf20Ti20Ni20 BMG. Our study advances the understanding of the atomic-scale origin of structure-property relationships in amorphous solids and provides a new strategy for ductilizing BMG without sacrificing strength.
Fast alignment and preprocessing of chromatin profiles with Chromap
Authors: Zhang Haowen , Song Li , Wang Xiaotao , Cheng Haoyu , Wang Chenfei , Meyer Clifford A. , Liu Tao , Tang Ming , Aluru Srinivas , Yue Feng , Liu X. Shirley , Li Heng 
Published: November 2021

AbstractAs sequencing depth of chromatin studies continually grows deeper for sensitive profiling of regulatory elements or chromatin spatial structures, aligning and preprocessing of these sequencing data have become the bottleneck for analysis. Here we present Chromap, an ultrafast method for aligning and preprocessing high throughput chromatin profiles. Chromap is comparable to BWA-MEM and Bowtie2 in alignment accuracy and is over 10 times faster than traditional workflows on bulk ChIP-seq/Hi-C profiles and than 10x Genomics’ CellRanger v2.0.0 pipeline on single-cell ATAC-seq profiles.
An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
Authors: Sabatier Pierre , Beusch Christian M. , Saei Amir A. , Aoun Mike , Moruzzi Noah , Coelho Ana , Leijten Niels , Nordenskjöld Magnus , Micke Patrick , Maltseva Diana , Tonevitsky Alexander G. , Millischer Vincent , Carlos Villaescusa J. , Kadekar Sandeep , Gaetani Massimiliano , Altynbekova Kamilya , Kel Alexander , Berggren Per-Olof , Simonson Oscar , Grinnemo Karl-Henrik , Holmdahl Rikard , Rodin Sergey , Zubarev Roman A. 
Published: November 2021

AbstractDetailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro.
Aborting meiosis allows recombination in sterile diploid yeast hybrids
Authors: Mozzachiodi Simone , Tattini Lorenzo , Llored Agnes , Irizar Agurtzane , Škofljanc Neža , D’Angiolo Melania , De Chiara Matteo , Barré Benjamin P. , Yue Jia-Xing , Lutazi Angela , Loeillet Sophie , Laureau Raphaelle , Marsit Souhir , Stenberg Simon , Albaud Benoit , Persson Karl , Legras Jean-Luc , Dequin Sylvie , Warringer Jonas , Nicolas Alain , Liti Gianni 
Published: November 2021

AbstractHybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.
Successful ageing among a national community-dwelling sample of older adults in India in 2017–2018
Authors: Pengpid Supa , Peltzer Karl 
Published: November 2021

AbstractThis study aimed to determine the prevalence and correlates of successful ageing in older community-dwelling adults in India. The cross-sectional sample included 21,343 individuals (≥ 65 years) from the Longitudinal Ageing Study in India (LASI) Wave 1 in 2017–2018. Successful ageing was assessed utilizing a multidimensional concept, including five components: (1) absence of major illness, (2) free of disability, (3) no major depressive disorder, (4) social engagement and (5) life satisfaction. Overall, 27.2% had successful ageing, including 83.3% had no major diseases, 51.0% free from disability, 91.8% had no major depressive disorder, 73.6% were socially engaged and 74.6% had high life satisfaction. In the adjusted logistic regression analysis, male sex (Adjusted Odds Ratio-AOR 1.40, 95% Confidence Interval-CI 1.21–1.26), married (AOR 1.48, 95% CI 1.22–1.79), having formal education (AOR 1.47, 95% CI 1.23–1.74), high subjective socioeconomic status (AOR 1.61, 95% CI 1.29–2.01), urban residence (AOR 1.42, 95% CI 1.19–1.70), Sikhs (AOR 1.76, 95% CI 1.38–2.24), high physical activity (AOR 1.65, 95% CI 1.38–1.97), and daily Yoga practice (AOR 1.34, 95% CI 1.11–1.61) increased the odds of successful ageing, while increasing age (AOR 0.96, 95% CI 0.94–0.79), poor childhood health (AOR: 0.47, 95% CI 0.29–0.75), and underweight (AOR 0.70, 95% CI 0.61–0.81) decreased the odds of successful ageing. Almost one in three older adults in India were successfully ageing. Factors associated with successful ageing included, male sex, married, having formal education, high subjective socioeconomic status, urban residence, Sikhs, physical activity, Yoga practice, younger age, good childhood health, and not having underweight.
Comparison of four healthy lifestyle scores for predicting cardiovascular events in a national cohort study
Authors: Tsai Ming-Chieh , Yeh Tzu-Lin , Hsu Hsin-Yin , Hsu Le-Yin , Lee Chun-Chuan , Tseng Po-Jung , Chien Kuo-Liong 
Published: November 2021

AbstractThe protective effect of different healthy lifestyle scores for the risk of cardiovascular disease (CVD) was reported, although the comparisons of performance were lacking. We compared the performance measures of CVDs from different healthy lifestyle scores among Taiwanese adults. We conducted a nationwide prospective cohort study of 6042 participants (median age 43 years, 50.2% women) in Taiwan’s Hypertensive, Hyperglycemia and Hyperlipidemia Survey, of whom 2002 were free of CVD at baseline. The simple and weighted the Mediterranean diet related healthy lifestyle (MHL) scores were defined as a combination of normal body mass index, Mediterranean diet, adequate physical activity, non-smokers, regular healthy drinking, and each dichotomous lifestyle factor. The World Cancer Research Fund and American Institute for Cancer Research cancer prevention recommended lifestyle and Life's Simple 7 following the guideline definition. The incidence of CVD among the four healthy lifestyle scores, each divided into four subgroups, was estimated. During a median 14.3 years follow-up period, 520 cases developed CVD. In the multivariate-adjusted Cox proportional hazard models, adherence to the highest category compared with the lowest one was associated with a lower incidence of CVD events, based on the simple (hazard ratio [HR] 0.43, 95% confidence interval [CI] 0.2–0.94) and weighted MHL scores (HR 0.44, 95% CI 0.28–0.68). Additionally, age played a role as a significant effect modifier for the protective effect of the healthy lifestyle scores for CVD risk. Specifically, the performance measures by integrated discriminative improvement showed a significant increase after adding the simple MHL score (integrated discriminative improvement: 0.51, 95% CI 0.16–0.86, P = 0.002) and weighted MHL score (integrated discriminative improvement: 0.38, 95% CI 0.01–0.74, P = 0.021). We demonstrated that the healthy lifestyle scores with an inverse association with CVD and reduced CVD risk were more likely for young adults than for old adults. Further studies to study the mechanism of the role of lifestyle on CVD prevention are warranted.
Limited thermal plasticity may constrain ecosystem function in a basally heat tolerant tropical telecoprid dung beetle, Allogymnopleurus thalassinus (Klug, 1855)
Authors: Machekano Honest , Zidana Chipo , Gotcha Nonofo , Nyamukondiwa Casper 
Published: November 2021

AbstractTropical organisms are more vulnerable to climate change and associated heat stress as they live close to their upper thermal limits (UTLs). UTLs do not only vary little across tropical species according to the basal versus plasticity ‘trade-off’ theory but may also be further constrained by low genetic variation. We tested this hypothesis, and its effects on ecosystem function using a diurnally active dung rolling beetle (telecoprid),Allogymnopleurus thalassinus(Klug, 1855) that inhabits arid environments. Specifically, (i) we tested basal heat tolerance (critical thermal maxima [CTmax] and heat knockdown time [HKDT]), and (ii) ecological functioning (dung removal) efficiency following dynamic chronic acclimation temperatures of variable high (VT-H) (28–45 °C) and variable low (VT-L) (28–16 °C). Results showed thatA. thalassinushad extremely high basal heat tolerance (> 50 °C CTmaxand high HKDT). Effects of acclimation were significant for heat tolerance, significantly increasing and reducing CTmaxvalues for variable temperature high and variable temperature low respectively. Similarly, effects of acclimation on HKDT were significant, with variable temperature high significantly increasing HKDT, while variable temperature low reduced HKDT. Effects of acclimation on ecological traits showed that beetles acclimated to variable high temperatures were ecologically more efficient in their ecosystem function (dung removal) compared to those acclimated at variable low temperatures.Allogymnopleurus thalassinusnevertheless, had low acclimation response ratios, signifying limited scope for complete plasticity for UTLs tested here. This result supports the ‘trade-off’ theory, and that observed limited plasticity may unlikely bufferA. thalassinusagainst effects of climate change, and by extension, albeit with caveats to other tropical ecological service providing insect species. This work provides insights on the survival mechanisms of tropical species against heat and provides a framework for the conservation of these natural capital species that inhabit arid environments under rapidly changing environmental climate.
Hatching phenology is lagging behind an advancing snowmelt pattern in a high-alpine bird
Authors: Schano Christian , Niffenegger Carole , Jonas Tobias , Korner-Nievergelt Fränzi 
Published: November 2021

AbstractTo track peaks in resource abundance, temperate-zone animals use predictive environmental cues to rear their offspring when conditions are most favourable. However, climate change threatens the reliability of such cues when an animal and its resource respond differently to a changing environment. This is especially problematic in alpine environments, where climate warming exceeds the Holarctic trend and may thus lead to rapid asynchrony between peaks in resource abundance and periods of increased resource requirements such as reproductive period of high-alpine specialists. We therefore investigated interannual variation and long-term trends in the breeding phenology of a high-alpine specialist, the white-winged snowfinch, Montifringilla nivalis, using a 20-year dataset from Switzerland. We found that two thirds of broods hatched during snowmelt. Hatching dates positively correlated with April and May precipitation, but changes in mean hatching dates did not coincide with earlier snowmelt in recent years. Our results offer a potential explanation for recently observed population declines already recognisable at lower elevations. We discuss non-adaptive phenotypic plasticity as a potential cause for the asynchrony between changes in snowmelt and hatching dates of snowfinches, but the underlying causes are subject to further research.
Magnetoactive acoustic metamaterials based on nanoparticle-enhanced diaphragm
Authors: Tang Xingwei , Liang Shanjun , Jiang Yusheng , Gao Cong , Huang Yujin , Zhang Yuan , Xue Chang , Wen Weijia 
Published: November 2021

AbstractMagnetoactive membrane-type acoustic metamaterials are fabricated by coating a layer of magnetic nanoparticles on the polyethylene (PE) membranes and their vibration characters are investigated experimentally. From our experiments, we discovered that, under different magnetic fields by varying the distance between a magnet and the membranes, such membranes exhibit tunable vibration eigenfrequencies (the shift towards lower frequencies), which is caused by the variation of the effective mass density and effective tension coefficient resulted from the second derivative of the magnetic field. The strong magnetic force between the layer of magnetic nanoparticles and the magnet enhances the eigenfrequency shift. A spring oscillator model is proposed and it agrees well with the experimental results. We also experimentally observed that the vibration radius, effective mass density, and effective tension coefficient of the membranes can enormously affect the eigenfrequencies of the membranes. We believe that this type of metamaterials may open up some potential applications for acoustic devices with turntable vibration properties.
Functional Communication Abilities in Youth With Cerebral Palsy: Association With Impairment Profiles and School-Based Therapy Goals
Authors: Koopmans Clare , Sakash Ashley , Soriano Jennifer , Long Helen L. , Hustad Katherine C. 
Published: November 2021

Purpose:
The aim of this study was to examine the relationship between functional communication skills, underlying speech, language, and cognitive impairments and school-based speech pathology services in students with cerebral palsy (CP).

Method:
Thirty-five participants with CP who had Individualized Education Programs (IEPs) were classified according to the Communication Function Classification System (CFCS). Participants completed laboratory assessments of speech, receptive language, executive functioning, and nonverbal cognition. Each participant's speech and language IEP goals were coded into treatment units and then categorized into seven, mutually exclusive target goal areas. Nonparametric analyses were employed to examine differences among CFCS groups in the number of deficit areas and the number of goal areas from the IEP. Descriptive analyses were used to evaluate the extent to which deficit and goal areas in the IEP co-occurred by CFCS level.

Results:
Those in more involved CFCS levels demonstrated more severe speech, receptive language, and cognitive impairments. However, there were no significant differences in the number of deficit areas across CFCS groups. Regardless of CFCS level, there were no differences in the number of treatment goals specified in the IEP. Literacy was the only goal area addressed across all CFCS levels. Those in the most involved CFCS levels had augmentative and alternative communication (AAC) goals, but those with more moderate restrictions in functional communication who also had markedly reduced speech intelligibility did not typically have speech or AAC goals.

Interpretation:
Individuals with CP across CFCS levels demonstrate variability in underlying deficit profiles, suggesting that measures of both functional communication and of underlying deficits are necessary to provide a complete picture of communication needs. Literacy goals were common across all CFCS levels, but AAC goals were limited to the most severely involved individuals, suggesting that continuing education may be necessary to support speech-language pathologists in developing treatments involving the integration of AAC and speech to foster functional communication at school.

Supplemental Material:

https://doi.org/10.23641/asha.16968073
Factors Influencing Oral Bolus Hold Type: Tipper or Dipper
Authors: Garand Kendrea L. (Focht) , Reilly Mary Catherine , Choi Dahye , Dey Rajarshi , Estis Julie , Hill Grayson 
Published: November 2021

Purpose:
This retrospective study investigated the impact of age, sex, and swallow task on bolus hold location during cued liquid swallows in healthy, community-dwelling adults. Furthermore, we investigated the association between bolus hold location and Modified Barium Swallow Impairment Profile (MBSImP) Components 2 (bolus hold) and 4 (lingual transport) scores.

Method:
Cued swallows of standardized barium liquids (thin, nectar-thickened, honey-thickened) observed during videofluoroscopy in 195 participants were judged as either tipper or dipper bolus hold type. Logistic regression was used to determine relationships between bolus hold location and age, sex, and swallow task. Fisher's exact test was used to determine associations between MBSImP scores and bolus hold type.

Results:
The majority of swallow tasks (95%) were of tipper bolus hold type. A significant relationship was observed between age and bolus hold type for swallows of 5-ml thin liquid and 5-ml honey-thickened liquid, with older individuals more likely to demonstrate a tipper bolus hold type. Sex was observed to be a significant factor for two swallow tasks (cup sip of thin liquid and 5 ml of nectar-thickened liquid), with males being more likely to be dippers. No significant relationships were observed between bolus hold type and MBSImP Component 2 or 4 scores.

Conclusions:
A tipper bolus hold type was the most common type observed in healthy adults, although the dipper bolus hold type was also observed (albeit at a lower frequency). These study findings further contribute to defining typical swallowing behaviors.
Fault tolerant control in an unmanned bicycle robot via sliding mode theory
Authors: Alizadeh Mousa , Ramezani Amin , Saadatinezhad Hadis 
Published: November 2021

AbstractIn this work, a new active fault tolerant control (FTC) is developed for an unmanned bicycle robot based on an integration between a sliding mode control (SMC), fault detection (FD), and fault estimation (FE) via a residual signal. A sliding surface in accordance with the fault tolerant sliding mode control (FTSMC) is designed for the bicycle robot to get multiple exciting features such as fast transient response with finite time convergence, small overshoot and quick stabilisation in the presence of an actuator fault. To obtain an effective performance for the FTSMC, a fault estimation system is employed and in order to attain estimation, an extended Kalman filter (EKF) as an estimator and a change detection algorithm called cumulative sum (CUSUM) as a residual evaluation function are developed. The innovative features of the proposed approach, that is FTSMC, are verified when compared with the other up‐to‐date control techniques like fault tolerant model‐based predictive control with feedback linearisation (FTMPC + FBL) and fault tolerant linear quadratic regulator with feedback linearisation (FTLQR + FBL) on an unmanned bicycle robot.
Hydroxyapatite nanomaterials with tailored length regulated by different fatty acids
Authors: Qi Mei‐li , Yao Shengkun , Wang Wen , Qi Liang , Wang Yanmin , Zhang Haijun 
Published: November 2021

AbstractThe morphology and structures of hydroxyapatite (HA) nanomaterials greatly influence their physicochemical properties and bio‐applications. In this study, HA nanostructures with tailored length regulated by different fatty acids were prepared via a solvothermal route. By using ricinoleic acid, linoleic acid and oleic acid as the template, the growth of HA crystals was regulated and the morphology of HA nanomaterials was transformed from long nanowires to short nanowires and then nanorods. The underlying mechanism for the formation of HA nanomaterials synthesized by different kinds of fatty acids under solvothermal condition was proposed. This study provides a simple and environmentally friendly way to obtain HA nanomaterials with tailored length for biomedical applications.
Online current cut‐off frequency self‐tuning active damping speed controller for permanent magnet synchronous motors
Authors: You Sung Hyun , Kim Ki‐Chan , Kim Seok‐Kyoon 
Published: November 2021

AbstractThis study suggests a dynamic current cut‐off frequency‐based pole‐zero cancellation speed controller for permanent magnet synchronous motors (PMSMs). The proposed self‐tuning algorithm automatically increases the current cut‐off frequency during only the transient periods and restores it as approaching the steady‐state operation. The outer loop control injects the active damping effect, resulting in a closed‐loop order reduction by pole‐zero cancellation from the particularly structured feedback gain. These two benefits contribute to the following advantages: (a) lowering the steady‐state current cut‐off frequency to improve the relative stability margin and (b) securing the capability of assigning the desired cut‐off frequency to both the inner and outer loops in the first‐order low‐pass filter form. A 500‐W PMSM experimental prototype platform confirms the effectiveness of the proposed controller.