bluuebunny/crossref_metadata_2025_split huggingface dataset:
Baicalin Regulates Autophagy to Intervene with Intestinal Acute Graft-Versus-Host Disease via AMPK/Mtor Pathway and Abnormal Intestinal Flora
Authors: sun xiaoqi , Pisano Michael , Xu Longjin , Sun Fumou , Xu Jie , Zheng Wei , Liu Xiujuan , Zhang Yanyu , Sun Runjie , Cui Xing 
Published: July 2021

Abstract
Acute graft versus host disease (aGVHD) is the main complication of allogeneic hematopoietic stem cell transplantation and the main cause of death. To verify if baicalin can protect the intestinal mucosal barrier by regulating abnormal autophagy and interfering with acute intestinal graft-versus-host disease, a mouse model of aGVHD was established. CB6F1 mice were transplanted with a mononuclear cell suspension from Balb/c donor mice bone marrow and spleen via vein injection after treatment with 60co X rays. After treatment with different doses of baicalin for 15 days, survival time, survival time,the serum level of TNF-α and IL-10, the autophagy markers from intestinal and bacterial flora were detected.A intestinal barrier dysfunction cell model was also used to verify the effect of baicalin.The results showed that baicalin could significantly prolong survival time, reduced aGVHD score compared to the model control group, significantly reduce TNF-α level, and increase IL-10 level. TEM examination showed that baicalin treatment increased the number of autophagic vacuoles and led to recovery of mitochondrial structure in the intestinal mucosal epithelial cells of mice and in Caco-2 cells. Western blot results showed that baicalin treatment enhanced autophagy in vivo by regulating the AMPK/mTOR autophagy pathway. Similar results were observed in vitro in Caco-2 cells. Furthermore, the effect of baicalin was reduced after combined treated with autophagy inhibitors 3-MA.The results of intestinal microbial sequencing also confirmed that there were significant changes in the type and quantity of bacterial flora before and after the application of baicalin. Baicalin can reduce the severity of intestinal acute graft-versus-host disease by regulating autophagy with influencing the unbalanced inflammatory cytolines and bacterial flora, which may be a new treatment for aGVHD.
Comparative Analysis of Optical Coherence Tomography and Ultrasound Biomicroscopy Imaging Diagnosis of Anterior Segment of Keratoconus in Acute Stage
Authors: zhao yun , guo jinxi , wang jianmin , xu jianfeng , xie ming , wei wei 
Published: July 2021

Abstract
Background To compare and analyze the imaging differences between anterior segment optical coherence tomography (AS-OCT) and ultrasound biomicroscopy (UBM) in acute keratoconus. Method The features of AS-OCT and UBM images of acute keratoconus patients in Hebei Eye Hospital were analyzed retrospectively. Result From May 2016 to December 2020, 38 patients (38 eyes) with acute keratoconus were treated in Hebei Eye Hospital. Both AS-OCT and UBM images showed rupture of the posterior elastic layer of cornea, edema and thickening of stroma layer, and cracks formed in it to communicate with anterior chamber. AS-OCT image showed that corneal epithelium was intact, and 26 cases had subcutaneous effusion. UBM images showed delamination of the posterior elastic layer interface in 26 cases. Paired t test showed that there was significant difference between preoperative and postoperative best corrected visual acuity in all patients (t = 11.861, P < 0.05). There was no significant difference in preoperative and postoperative best corrected visual acuity between group 1 and group 2 by independent sample t test (t = 0.751, P> 0.05, t = 0.219, P > 0.05). There was significant difference in the height of rupture between group 1 and group 2 (t =-7.533, P < 0.001). Pearson correlation analysis showed that there was a significant correlation between fracture size and fracture height (r = 0.766, P < 0.001), but no correlation between fracture size and post-elastic layer delamination (r =-0.107, P > 0.05). No corneal perforation was found in 38 cases (38 eyes). In the healing stage after deep lamellar keratoplasty, obvious stroma scar appeared, accompanied by corneal stroma thickening and corneal flattening. Conclusion AS-OCT and UBM can complement each other, provide accurate corneal imaging images and parameter measurement, and predict and evaluate the development of the disease.
Psychological Impacts of the COVID-19 Pandemic on One-month Postpartum Mothers: A Retrospective Study from a Consecutive Database in a Metropolitan Area of Japan
Authors: Takubo Youji , Tsujino Naohisa , Aikawa Yuri , Fukiya Kazuyo , Iwai Momoko , Uchino Takashi , Ito Megumu , Akiba Yasushi , Mizuno Masafumi , Nemoto Takahiro 
Published: July 2021

Abstract
Background:The coronavirus disease 2019 (COVID-19) pandemic has recently become the most important issue in the world. Very few reports in Japan have examined the impact of the COVID-19 pandemic on peripartum mental health. We examined the current status of postpartum mental health in a metropolitan area.Methods:The subjects were women who had completed a maternity health check-up at a core regional hospital in Yokohama during the period from April 1, 2017, to December 31, 2020. We collected the subjects’ scores for the Edinburgh Postnatal Depression Scale (EPDS) and the Mother-to-Infant Bonding Scale (MIBS) at one month postpartum. The subjects were divided into four groups (three Before COVID-19 groups and a During COVID-19 group). MANOVA and post-hoc tests were used to determine mental health changes in the postpartum period among the four groups.Results:The Before and During COVID-19 groups contained 2844 and 1095 mothers, respectively. There were no significant difference in the total scores of the EPDS and MIBS among the four groups. However, the EPDS items related to anxiety factors were significantly higher and the EPDS items related to anhedonia and depression factors (excluding thoughts of self-harm) were significantly lower in the During COVID-19 group.Conclusion:The EPDS scores changed in connection with the COVID-19 pandemic. Anxiety, which represent hypervigilance, was significantly higher and anhedonia and depression were significantly lower in the During COVID-19 group. Our results may reflect COVID-19-related health concerns and a lack of social support caused by the COVID-19 pandemic.
Analysis of Bronchial And Vascular Patterns In Left Upper Lobes To Explore The Genesis of Mediastinal Lingular Artery And Its Influence On Pulmonary Anatomical Variation
Authors: Gao Chuan , Xu Wen-zheng , Li Zhi-hua , Chen Liang 
Published: July 2021

Abstract
Background: For thoracic surgeons, three-dimensional computed tomography bronchography and angiography (3-DCTBA) is a convenient way to analyze pulmonary variations before segmentectomy. Mediastinal lingular artery (MLA) is one of the representative variations Methods: The 3-DCTBA data of left upper lobe（LUL）were collected from patients who underwent pulmonary surgery from January 2018 to December 2019. We reviewed the patterns of bronchi and pulmonary vessels and grouped them according to different classifications. Results: Among all the 404 cases of 3-DCTBA, mediastinal lingular artery (MLA) was found in 107 cases (26.49%). The patterns of B3 and the vein in left upper division (LUD) are distinct between mediastinal (M-type) group and interlobar (IL-type) group. The patterns of bronchi and veins in lingular division, as well as the pattern of pulmonary artery in LUD, have no differences between M-type and IL-type groups. Conclusions: Mediastinal lingular artery is speculated to originate from the variation of B3, and the MLA independently influences the venous pattern in LUD in turn.
Resuming In-Person Psychotherapy Sessions with Perinatal Women amid the COVID-19 Pandemic: A Multistakeholder Perspective
Authors: Andrejek Nicole , Hossain Sabrina , Schoueri-Mychasiw Nour , Saeed Gul , Zibaman Maral , Niño Angie Puerto , Meltzer-Brody Samantha , Silver Richard , Vigod Simone N. , Singla Daisy Radha 
Published: July 2021

Abstract
BACKGROUND: During the COVID-19 pandemic, essentially all outpatient psychotherapy with perinatal patients transitioned to telemedicine. The aim of this study was to examine how to resume in-person psychotherapy with perinatal patients within the Scaling Up Maternal Mental healthcare by Increasing access to Treatment (SUMMIT) trial as the pandemic abates. METHODS: We conducted focus groups and individual interviews with perinatal participants (n=23), providers (n=28), and a range of stakeholders (n=18) who participated in the SUMMIT trial from August 2020 to March 2021. Content analysis was used to examine (1) previous barriers that impacted perinatal participants access or decision to access in-person healthcare during COVID-19; (2) perceived future barriers for perinatal participants to attend in-person sessions; and (3) potential facilitators to resuming in-person sessions with perinatal participants. RESULTS: Perinatal participants (69.6%) and providers (57.1%) reported that COVID-19 previously impacted perinatal SUMMIT patients’ access or decision to access in-person healthcare. Specifically, “external barriers” such as institutional regulations due to COVID-19 (100% perinatal participants and 68.8% providers) and “internal barriers” such as concerns about being exposed to COVID-19 (75.0% perinatal participants and 56.3% providers) impacted perinatal participants access to in-person care. Perceived barriers to resuming in-person psychotherapy sessions included concerns about virus exposure in a hospital setting (77.8% stakeholders, 73.9% perinatal participants, 71.4% providers); virus exposure while taking public transportation (50.0% stakeholders, 26.1% perinatal participants, 25.0% providers). Stakeholders (50.0%) were more likely than providers (25.0%) and participants (13.0%) to endorse wearing a mask during sessions as a barrier. Key facilitators to resuming in-person psychotherapy sessions were the implementation and effective communication of robust safety protocols (72.2% stakeholders, 47.8% perinatal participants, 39.3% providers) and providing alternative locations to where sessions are delivered, e.g., using large rooms (44.4% stakeholders, 32.1% providers, 17.4% perinatal participants). CONCLUSION: The results suggest that a significant majority of perinatal participants experienced barriers to accessing in-person healthcare during the pandemic, and concerns about contracting the virus within and en route to the hospital remain while the COVID-19 pandemic persists. To overcome these barriers, our study suggests that implementing and effectively communicating robust safety protocols may be the most effective methods to address these concerns when resuming in-person psychotherapy sessions.
Antibacterial Activity of Biosynthesized Ferric Oxide Nanoparticles Using Cissus Quadrangularis Extract
Authors: Anandam Sankareswari , Indira K. 
Published: July 2021

Abstract
Green chemistry plays a significant role in protecting and improving the global environment. Green chemistry is a process in which a reducing agent usually a chemical is replaced by an extract of a natural product such as stems, leaves, fruits and its peels etc., of plants for the synthesis of nanoparticles. The metal and metal oxide nanoparticles produced from a plant extract are usually stable for a period of time. The present research work focuses on the synthesis of iron oxide [Fe2O3] nanoparticles using Cissus Quadrangularis commonly known as veldt grape (pirandai in Tamil) plant extract by precipitation method. The structural, surface morphological and optical properties of the synthesized Fe2O3 nanoparticles were studied by XRD, SEM and UV-Vis spectroscopy. Further the antibacterial activity of the prepared Fe2O3 nanoparticles was studied against gram - negative bacteria (Salmonella and Escherichia coli) and gram - positive bacteria (Bacillus subtili and Staphylococcus aureus) by disc diffusion method. X-ray diffraction analysis reveals that the prepared nanoparticles have hexagonal wurtzite structure with preferential orientation along (1 0 4) plane. The crystalline size of the synthesized Fe2O3 nanoparticles is 12.324 nm. SEM analysis shows that iron oxide nanoparticles have pebble shape. EDX result confirms the presence of Fe and O in the prepared Fe2O3 nanoparticle samples. The band gap value of biosynthesized Fe2O3 nanoparticles is 2.57eV. Antibacterial studies reveal that the ferric oxide nanoparticles display significant inhibition activities against all tested pathogens.
Band Gap Analysis of Ca3MgSi2O8:Dy3+ Nanorod Arrays By A Solid State Reaction Method
Authors: Dewangan Pradeep , Bisen D. P. , Brahme Nameeta , Sharma Shweta , Tamrakar Raunak Kumar , Upadhyay Kanchan 
Published: July 2021

Abstract
Present study throuws light on solid state synthesis, phase and band properties of a Dy3+ doped Ca3MgSi2O8. Crystal phase of the phosphor was characterized by X-ray diffraction (XRD) and surface morphology was crystal size was determined by using scanning elelctron microscopy (SEM), and transmission electron microscopy (TEM) respectively. To detemine the band gap behaviour of the crystal absorption spectra was used, which was further analyzed by using Tauc plot. The band gap is calculated ≈4.5eV, which decreases on increasing doping concentration of Dy3+.
Seasonal Variation of Atmospheric Carbon Dioxide Over Suburban Site of Pune, India
Authors: Patil M N , Dharmaraj T , Waghmare R T , Pawar S D 
Published: July 2021

Abstract
Increase in greenhouse gases including carbon dioxide (CO2) concentrations in the atmosphere, is the responsible factor for the global warming. This increase is caused by the anthropogenic activities like combustion of petroleum products, biomass burning, etc. In recent years, measurement network of greenhouse gases has been expanded globally. In this study, we measured the CO2 and water vapor (H2O) concentrations along with wind and air temperature over suburban site of Pune, in the year of 2018-2019 comprising the four seasons : pre-monsoon (months of March, April and May (MAM)), summer monsoon (months of June, July, August and September (JJAS)), post-monsoon (months of October and November (ON)) and winter (months of December, January and February (DJF)). Using these observations, the diurnal and seasonal variations of CO2 concentrations and fluxes were analyzed. The mean CO2 concentration was observed in the range of 380.7 – 443.6 ppm. The seasonal mean of CO2 concentration was 400, 421.5, 395.4 and 384.7 ppm in pre-monsoon, monsoon, post-monsoon and winter seasons, respectively. It is found that the height of planetary boundary layer and photosynthesis process plays a major role in seasonal variation of CO2.
The Effects of Rosmarinic Acid on Expression of Akt Genes in the Hippocampus of Diabetic Rats and DNA Glycation Inhibition.
Authors: Alrubaye Ameer , Motovali-Bashi Majid , Miroliaei Mehran 
Published: July 2021

Abstract
Non-enzymatic glycation of DNA and the associated effects are among pathogenic factors in diabetes mellitus. Natural polyphenols have anti-diabetic activity. Herein, the protective role of one of the phytochemicals, rosmarinic acid (RA), was evaluated in glycation (with fructose) of human DNA and expression of Akt genes in the hippocampus of diabetic rats. In-vitro studies using fluorescence, agarose gel electrophoresis, fluorescence microscopy, and thermal denaturation analyses revealed that glycation causes DNA damage and that RA inhibits it. In-vivo studies were performed by induction of diabetes in rats using streptozotocin. The diabetic rats were given RA daily through gavage feeding. The expression of Akt genes (inhibitors of apoptosis) in the hippocampus was evaluated using RT-qPCR. In diabetic rats, Akt1 and Akt3 were significantly down-regulated compared to the control group. Treating the diabetic rats with RA returned the expression of Akt1 and Akt3 relatively to the normal condition. Past studies have shown that diabetes induces apoptosis in the hippocampal neurons. Given that glycation changes the genes expression and causes cell death, apoptosis of the hippocampal neurons can be due to the glycation of DNA. The results also suggest that RA has reliable potency against the gross modification of DNA under hyperglycemic conditions.
Identifying sex-specific genetic effects across 733 traits in UK Biobank
Authors: Han James , Jiang Wei , Ye Yixuan , Zhao Hongyu 
Published: July 2021

Abstract
Sex-specificity has been reported in a wide range of diseases and complex traits. While sex-specific genetic effects have been documented for certain traits, the genetic mechanisms underlying sex differences in most traits remain largely unexplored. With its large sample size and wide range of diseases and traits, the UK Biobank—a large, prospective cohort study containing health history, phenotypic measurements, and genetic data for over 500,000 individuals—provides an opportunity to explore sexually dimorphic genetic architectures in a large number of traits and diseases. Here, we present a sex-specific analysis of 733 sex-stratified complex trait GWAS for 361,194 white British individuals in the UK Biobank, and report 16 traits with significant sex-specific differences in heritability. These 16 candidate traits with sex-specific genetic effects belong to 5 distinct groups: body fat mass and distribution, blood pressure, creatinine levels, snoring, and birth weight. Using a systematic sex-specific discovery-replication analysis, we identify 47 (31 novel) loci showing sex-specific effects on the traits related with body fat mass/distribution, blood pressure, and birth weight, and discover 74 potential sex-specific biological pathways from the enrichment analyses based on associated genes from QTL analysis. In addition, we present further evidence for significant sex-specific genetic effects in 13 traits spanning three trait groups (body fat mass/distribution, blood pressure, and birth weight) by comparing the prediction performance of sex-specific polygenic risk scores.
Long Non-coding RNA CBR3-AS1 Mediates Tumorigenesis and Radiosensitivity of Non-small Cell Lung Cancer through Redox and DNA Repair by CBR3-AS1 /miR-409-3p/SOD1 Axis
Authors: Liu Shilong , Zhan Ning , Gao Chunzi , Xu Piao , Wang Hong , Wang Siyu , Piao Shiqi , Jing Suwei 
Published: July 2021

Abstract
Background: Long noncoding RNA (lncRNA) CBR3-AS1 has important functions in various cancers. However, the biological functions of CBR3-AS1 in non-small lung cancer (NSCLC) remains unclear. This study aimed to investigated the roles and molecular mechanisms of lncRNA CBR3-AS1 in NSCLC tumorigenesis and radiosensitivity. Methods: The association of CBR3-AS1 expression with the clinicopathological features and prognosis of NSCLC was studied based on The Cancer Genome Atlas database and verified in tumor and adjacent normal tissues of 48 NSCLC patients. The effect of CBR3-AS1 knockdown or overexpression on proliferation, apoptosis, migration, and invasion of NSCLC cells were studied through CCK-8, flow cytometry, and transwell assays, and tumorigenesis experiments in nude mice. The interactions of CBR3-AS1, miR-409-3p, and SOD1 were investigated using double luciferase reporter gene. The effect of CBR3-AS1 on NSCLC radiosensitivity was verified using clone formation assay and the single-click multi-target mathematical model. Changes in γH2AX level and reactive oxygen species (ROS) levels were detected using immunofluorescence and flow cytometry in NSCLC cells after irradiation.Results: CBR3-AS1 expression in NSCLC tissue was increased significantly compared with that of surrounding normal tissue. Cell function experiments verified that CBR3-AS1 downregulation could reduce proliferation, invasion, and migration of NSCLC cells and inhibit cell cycle progression and promote apoptosis. The tumorigenesis experiment in nude mice showed that CBR3-AS1 promoted tumor growth. CBR3-AS1 could regulate the expression and functions of the target gene SOD1 of miR-409-3p. High CBR3-AS1 expression in NSCLC was negatively correlated with radiosensitivity. CBR3-AS1 downregulation in NSCLC may depress the expression of SOD1 after irradiation, promote the formation of γH2AX, increase levels of ROS, and increase apoptosis.Conclusions: CBR3-AS1 is highly expressed in NSCLC, and play oncogene function through the CBR3-AS1/miR-409-3p/SOD1 pathway. Downregulation of CBR3-AS1 led to enhanced radiosensitivity, thus providing a new therapeutic target for NSCLC.
Soluble IL-2R Levels Predict in-Hospital Mortality in COVID-19 Patients with Respiratory Failure
Authors: Jang Hye Jin , Leem Ah Young , Chung Kyung Soo , Ahn Jin Young , Jung Ji Ye , Kang Young Ae , Park Moo Suk , Kim Young Sam , Lee Su Hwan 
Published: July 2021

Abstract

Acute respiratory distress syndrome is the primary cause of death in patients with coronavirus disease 2019 (COVID-19) pneumonia. Our study aims to determine the association between serum markers and mortality in COVID-19 patients with respiratory failure. This retrospective study was conducted in a tertiary care hospital in South Korea. Forty-three patients with COVID-19, who required high flow nasal cannulation or mechanical ventilation from Feb 2020-Jan 2021, were included. Demographic and laboratory data were analyzed at baseline and on Day 7 of admission. Older age was associated with mortality. Serum creatinine, troponin, procalcitonin, and soluble interleukin-2 receptor (sIL-2R) at baseline were more elevated in the non-survivor group; however, were not associated with mechanical ventilator use on Day 7 PaO2/FiO2 ratio, lymphocyte and platelet counts, lactate dehydrogenase, IL-6, c-reactive protein, and sIL-2R on Day 7 were significantly associated with mortality. Delta sIL-2R (Day 7-Day 0) per standard deviation was significantly higher in the non-survivor group (adjusted hazard ratio 3.225, 95% CI 1.151-9.037, p=0.026). sIL-2R could predict mortality in COVID-19 patients with respiratory failure, not mechanical ventilator use. Its sustained elevation suggests a hyper-inflammatory state, and mirrors the severity of COVID-19 in patients with respiratory failure; therefore, warrants further attention.
Multi-Agent System for Resource Adaptation in IoT to Monitor Leakage in Irrigation Tanks
Authors: Hatti Daneshwari I. , Sutagundar Ashok Veerappa 
Published: July 2021

Abstract
The sporadic changes in the requirements by the end users has led to the problems in managing the resources of IoT devices. The problem of managing the heterogeneous requests with the available resources in view of ensuring Quality of service (QoS) to end users is challenging issue. The proposed model inculcates the adaptation policy for adapting the resources to fulfill the requirements of the user. The dynamic changes in the environment are handled by Reinforcement learning model with Fuzzy Interference system to apply the policy. Periodically monitoring of irrigation tank to alert the flow of water above Full Tank Level (FTL) by Reinforcement learning agent, prioritization of tasks (requests) by Fuzzy logic is performed. It is done by interacting through agency, providing video conferencing or video calling facility to the user based on availability of the user resources. It also adapts according to communication and computational resources. The proposed model is simulated to monitor and control the leakage in tank. It helps to remotely control the leakages in irrigation tanks/ bridges through Multi-Agent Fuzzy Q learning model. It focuses on adapting the resources of the devices and the action considering the user resources. The algorithm is simulated in Ifogsim and python and performance are evaluated in terms of resource cost, latency, execution time, energy consumption and network usage.