bluuebunny/crossref_metadata_2025_split huggingface dataset:
MRI Radiomics to Differentiate Between Low Grade Glioma and Glioblastoma Peritumoral Region
Authors: Malik Nauman , Geraghty Benjamin , Dasgupta Archya , Maralani Pejman Jabehdar , Sandhu Michael , Detsky Jay , Tseng Chia-Lin , Soliman Hany , Myrehaug Sten , Husain Zain , Perry James , Lau Angus , Sahgal Arjun , Czarnota Gregory 
Published: August 2021

Abstract
Background
The peritumoral region (PTR) of glioblastoma (GBM) appears as a T2W-hyperintensity and is characterized by microscopic tumor and edema. Infiltrative low grade glioma (LGG) comprises tumor cells that seem similar to GBM PTR on MRI. The work here explored if a radiomics-based approach can distinguish between LGG and GBM PTR, which can have future implications on existing treatment paradigms.
Methods
Patients with GBM and LGG imaged using a 1.5 T MRI were included in the study. Image data from cases of GBM PTR, and LGG were manually segmented guided by T2W hyperintensity. A set of 91 first-order and texture features were determined from each of T1W-contrast, and T2W-FLAIR, diffusion-weighted imaging sequences. Applying filtration techniques, a total of 3822 features were obtained. Different feature reduction techniques were employed, and a subsequent model was constructed using four machine learning classifiers. Leave-one-out cross-validation was used to assess classifier performance.
Results
The analysis included 42 GBM and 36 LGG. The best performance was obtained using AdaBoost classifier using all the features with a sensitivity, specificity, accuracy, and area of curve (AUC) of 91%, 86%, 89%, and 0.96, respectively. Amongst the feature selection techniques, the recursive feature elimination technique had the best results, with an AUC ranging from 0.87 to 0.92. Evaluation with the F-test resulted in the most consistent feature selection with 3 T1W-contrast texture features chosen in over 90% of instances.
Conclusions
Quantitative analysis of conventional MRI sequences can effectively demarcate GBM PTR from LGG, which is otherwise indistinguishable on visual estimation.
A miR-9-5p/FOXO1/CPEB3 feed-forward loop drives the progression of hepatocellular carcinoma
Authors: Hu Hui , Huang Wei , Li Jianye , Zhang Qiong , Miao Ya-Ru , Hu Fei-Fei , Gan Lu , Yang Xiangliang , Guo An-Yuan 
Published: August 2021

Abstract
Background

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide, but its regulatory mechanism remains unclear. Although many TFs and miRNAs are reported to be important in HCC, their co-regulation and FFL modules in HCC development are needed to be investigated.
Methods

The feed-forward loop (FFL) regulatory module was identified by analyzing the miRNA and transcription factor co-regulatory network for differentially expressed genes between tumors and matched adjacent tissue samples. Gene expression and regulatory role of HCC development by key FFL in vitro and in vivo were validated by qPCR, Western blotting, cell proliferation assay, migration and invasion assay and experiments in nude mice with hepatoma xenografts.
Results

Here, by bioinformatics analysis, we identified FFL regulatory module miR-9-5p/FOXO1/CPEB3 may play critical roles in HCC progression. Gain- and loss-of-function studies demonstrated that miR-9-5p promote hepatocarcinogenesis, while FOXO1 and CPEB3 inhibit hepatocarcinoma growth. Furthermore, CPEB3 was firstly identified as a direct downstream target of miR-9-5p and FOXO1 by luciferase reporter assay and ChIP-Seq data, which was negatively regulated by miR-9-5p and activated by FOXO1. Following, the miR-9-5p/FOXO1/CPEB3 FFL was associated with poor prognosis and promoted cell growth and tumorigenesis of HCC in both in vitro and in vivo experiments.
Conclusion

Our study newly identified the existence of miR-9-5p/FOXO1/CPEB3 FFL and revealed its regulatory role in HCC progression, which may represent a new potential therapeutic target for cancer treatment.
Waist-to-Height Ratio, An Optimal Anthropometric Indicator in Prediction of Metabolic Dysfunction Associated Fatty Liver Disease: Results From A Chinese Male Population Survey
Authors: cai jinwei , Lin Cuiting , Lai Shuiqing , Liu Yingshan , Liang Min , Qin Yingfen , Liang Xinghuan , Tan Aihua , Gao Yong , Lu Zheng , Wu Chunlei , Shengzhu Huang , Yang Xiaobo , Zhang Haiying , Kuang Jian , Mo Zengnan 
Published: August 2021

Abstract
BackgroundLimited available evidence implicated that the anthropometric indicators of adiposity may contribute to the predictive and diagnosis capability of non-alcoholic fatty liver disease (NAFLD) in a facile, low-cost and noninvasive way while NAFLD had been entitled as metabolic-dysfunction associated fatty liver disease (MAFLD) in 2020. This study aimed to validate and compare the predictive and diagnostic capability of eight anthropometric indicators in MAFLD individuals and determined an optimal diagnostic predictor for MAFLD, which may beneficial for resource scarce regions.MethodsThe retrospective cross-sectional population-based study conducted from Fangchenggang Area Male Health and Examination Survey (FAMHES) involved 2428 participants whose comprehensive questionnaire, detailed data, anthropometric parameters and biochemical measurements were collected. Eight anthropometric indicators including body mass index (BMI), waist-to-height ratio (WHtR), waist-hip ratio (WHR), body adiposity index (BAI), cardiometabolic index (CMI), lipid accumulation product (LAP), visceral adiposity index (VAI) and Abdominal volume index(AVI) were enrolled into analyzed. Receiver operating characteristic (ROC) curve analysis and the area under the ROC curves (AUCs) were used to compare the diagnostic ability of each indicator for MAFLD and optimal cut-off points fully determined. Binary logistic regression analysis was used to explore associations of all anthropometric indicators and MAFLD by determined the odds ratios (ORs) and 95% confidence interval (CI).ResultsAll selected anthropometric indicators were substantially associated with MAFLD (p < 0.001) and their diagnostic value of MAFLD by AUCs fully above 0.79. WHtR [OR 22.181(95%CI, 16.216–30.340)] had the strongest association with MAFLD, regardless of potential confounders. LAP had the largest AUC [0.868(95%CI 0.853–0.883)], intimately followed by WHtR [0.863(95%CI 0.848–0.879)]. AVI with OR 19.275(95%CI 14.266–26.041) and AUC 0.859 (95% CI 0.843–0.874) ranked second and third respectively among all indicators. The cut-off points of WHtR, LAP and AVI were 0.49, 24.29, and 13.61, separately.ConclusionAll eight anthropometric indicators illustrated the superior diagnostic value of MAFLD. Comparatively, WHtR might stressed as the most powerful diagnostic predictor for MAFLD, follow by LAP and AVI which presented highly potential as well.
Identification of a High-Risk Group for Brain Metastases in Non-Small Cell Lung Cancer Patients.
Authors: Cacho-Díaz Bernardo , Laura D. Cuapaténcatl , Rodriguez Jose Antonio , Garcilazo-Reyes Ytel Jazmin , Reynoso-Noverón Nancy , Arrieta Oscar 
Published: August 2021

Abstract
Purpose: Identification of a truly high-risk group of brain metastases (BM) in patients with non-small cell lung cancer (NSCLC) could lead to early interventions and probably better prognosis. The objective of the study was to identify this group by generating a multivariable model with recognized and accessible risk factors.Methods: A retrospective cohort from patients seen at a single center during 2010-2020, was divided into a training (TD) and validation (VD) datasets, associations with BM were measured in the TD with logit, variables significantly associated were used to generate a multivariate model. Model´s performance was measured by 10-Fold cross validation of the TD and in the VD with the AUC/C-statistic, Akaike information index, and Press´s Q precision test.Results: From 570 patients with NSCLC with complete records a TD and VD were formed, no significant differences were found amid both datasets. Variables associated with BM in the multivariate logit analyses were age [P 0.021, OR 0.97 (95%CI 0.94-0.99)]; male gender [P 0.010, OR 2.44 (95%CI 1.24-4.82)]; mutational status [P 0.03, OR 2.1 (95%CI 1.07-4.12); and carcinoembryonic antigen levels [P 0.002, OR 1.002 (95%CI 1.001 – 1.003). BM were diagnosed in 23% of the whole cohort. Stratification into low, medium, or high-risk groups after simplification of the model, displayed a frequency of BM in the VD of 8%, 16% and 40% respectively (P 0.027).Conclusion: A multivariate model comprising age, gender, CEA, and mutation status allowed the identification of a truly high-risk group of BM in NSCLC patients.
Neuroinflammatory Cytokine Response, Neuronal Death and Microglial Proliferation in the Hippocampus of Rats During the Early Period After Lateral Fluid-Percussion-Induced Traumatic Injury of the Neocortex
Authors: Komoltsev Ilia G. , Tret’yakova Liya V. , Frankevich Stepan O. , Shirobokova Natalia I. , Volkova Aleksandra A. , Butuzov Alexey V. , Novikova Margarita R. , Kvichansky Alexey A. , Moiseeva Yulia V. , Onufriev Mikhail V. , Bolshakov Alexey P. , Gulyaeva Natalia 
Published: August 2021

Abstract
Time course of changes in neuroinflammatory processes in the dorsal and ventral hippocampus was studied during the early period after lateral fluid-percussion-induced neocortical traumatic brain injury (TBI) in the ipsilateral and contralateral hemispheres. In the ipsilateral hippocampus, neuroinflammation (increase in expression of pro-inflammatory cytokines) was evident from day 1 after TBI and ceased by day 14, while in the contralateral hippocampus it was mainly limited to the dorsal part on day 1. TBI induced an increase in hippocampal corticosterone level on day 3 bilaterally and an accumulation of Il1b on day 1 in the ipsilateral hippocampus. Activation of microglia was observed from day 7 in different hippocampal areas of both hemispheres. Neuronal cell loss was detected in the ipsilateral dentate gyrus on day 3 and extended to the contralateral hippocampus by day 7 after TBI. The data suggest that TBI results in distant hippocampal damage (delayed neurodegeneration in the dentate gyrus and microglia proliferation in both the ipsilateral and contralateral hippocampus), the time course of this damage being different from that of the neuroinflammatory response.
Prognosis of patients with brainstem glioblastoma based on ‘age, surgery and radiotherapy’: a SEER database analysis
Authors: Li Yitong , Beeraka Narasimha M. , Guo Wenchang , Lei Yuying , Hu Qilu , Guo Litao , Fan Ruitai , Liu Junqi , Sui Aixia 
Published: August 2021

Abstract
Primary brainstem glioblastoma is a rare tumor with dismal prognosis that poses significant treatment challenges. The purpose of the current study is to identify and determine prognostic factors associated with the survival in the brainstem glioblastoma patients. We gathered the data from SEER database (1973–2016) to examine the survival of patients with brainstem glioblastoma and potential impact of demographic, tumor and clinical characteristics on the overall survival of the patient. The survival patterns were assessed using Kaplan–Meier curves and Cox proportional hazards models. Propensity score matching (PSM) analysis was performed between patients with or without radiation therapy based on age and surgical resection to investigate the effect of radiotherapy on the overall survival. Total 232 patients were included from the SEER database. The median overall survival was 8 months. Kaplan–Meier survival analysis delineated that thepatients with younger age (P = 0.001) and surgery (P = 0.001) exhibited better prognosis. Among 232 patients, 204 patients received radiotherapy (radiotherapy group, RG), and only 28 patients did not receive radiotherapy (non-radiotherapy group, NRG). Radiotherapy was associated with an improvement of overall survival without statistical significance (P = 0.104). PSM was performed between the RG and NRG based on age and surgical resection. After the PSM, 56 patients were included. Overall Survival was significantly different between both groups (P = 0.038, p < 0.05). Multivariate analysis showed that treatment with surgery and radiotherapy were considered as the independent prognostic factors (P < 0.05).
Outcomes of Evisceration or Enucleation by Resident Trainees in Patients with Endophthalmitis or Panophthalmitis
Authors: Tianthong Wannaporn , Aryasit Orapan 
Published: August 2021

Abstract
Background

To evaluate the outcomes of evisceration or enucleation with primary implantation performed by ophthalmology resident trainees in patients with endophthalmitis or panophthalmitis.
Methods

In this retrospective analysis, the clinical records of all patients diagnosed with endophthalmitis or panophthalmitis who underwent enucleation or evisceration with primary implantation over a 13-year period were reviewed. The predictive factors related to implant exposure or extrusion were identified using multivariate analysis.
Results

Sixty-six patients, with a median age of 46.8 years, were enrolled. Thirty-six (55%) out of 66 patients were diagnosed with panophthalmitis. The most common causes of endophthalmitis or panophthalmitis were trauma (13 patients) and corneal ulcer perforation (10 patients). Enucleation was performed in 53 patients (80%). Four patients (6%) had implant exposure, and 4 patients (6%) had implant extrusion. Multivariate analysis demonstrated that Pseudomonas aeruginosa infection (P = 0.021, adjusted odd ratio [aOR] 33.75) and not receiving intravitreal antimicrobial drugs before the eye removal procedure (P = 0.02, aOR = 30.11) were associated with implant exposure or extrusion. Patients with panophthalmitis who underwent evisceration had a higher rate of implant exposure or extrusion than those who underwent enucleation (P = 0.031, aOR 38.38). Other complications included socket discharge in 14 patients, lower lid laxity in 5 patients who underwent the lateral tarsal strip procedure, and wound dehiscence in 3 patients. At the last visit, 65 patients had successful prosthesis fitting.
Conclusion

This study suggests that evisceration or enucleation with primary implant placement in patients with recalcitrant endophthalmitis or panophthalmitis can be performed by resident trainees with acceptable surgical outcomes and a low rate of serious complications.
Bullying and its Effective Factors in Adolescents Aged Between 10 and 18 Years Old: Evidence From a Southeastern Region in Iran, 2020
Authors: Radmerikhi Samera , Feyzabadi Vahid Yazdi , Ahmadi Milad , Sajadi Seyyid Mohammad Keyhan 
Published: August 2021

Abstract
Background & Objective: Bullying behavior in adolescence as a global and a public health problem is of great interest in adolescence due to mental illness, violence, and tobacco addiction in adulthood. The present study aims to investigate the prevalence of both bullying and victim behaviors at all educational levels in Kerman. Methodology: This is a survey study, in which the study population are all students at three educational levels in Kerman. Multi-stage sampling method is used. To investigate the prevalence of bullying behavior, the Iranian version of the questionnaire is used, which is based on the Olweus bullying questionnaire. Results: The bullying rates at elementary, middle school, and high school are estimated at 25.1%, 17.8%, and 39.7%, respectively. Bullying rate is 7.13% among girls and 14.72% among boys. The results of our study show that the most important factors affecting bullying are gender, parental literacy, living with parents, type of school, economic and social status, educational level, and school size. Conclusion: Due to the impact of policies at different educational levels, policymakers should pay special attention to perform bullying control programs at the school level based on the school environment variables, and policies in this area should be designed, reviewed, and modified.
Synthesis, Magnetism and Photoluminescence of Mn Doped AlN Nanowires
Authors: Wang Qiushi , Yang Li , Li Junhong , Yang Huadong , Feng Zhenbao , Ge Zhu , Zhang Jian , Lin Yao , Wang Xuejiao , Wang Chuang , Fu Ying , Liu Cailong 
Published: August 2021

Abstract
Manganese doped aluminum nitride (AlN:Mn) nanowires were fabricated by direct nitridation of Al and Mn mixed powders. The as-synthesized AlN:Mn nanowires were characterized by X-ray diffraction, Raman, energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, scanning and transmission electron microscopy. The measurements reveal the successful incorporation of Mn2+ ions into AlN nanowires. The magnetic and optical properties of the AlN:Mn nanowires were studied using vibrating sample magnetometer and photoluminescence spectroscopy. The AlN:Mn nanowires exhibit room temperature ferromagnetic behavior and a red emission band centered at 597 nm corresponding to the 4T1(4G)-6A1(6S) transition of Mn2+. The abnormal thermal quenching behavior and long afterglow feature are investigated through the temperature dependent emission and the afterglow spectrum. The thermoluminescence curve shows that AlN: Mn nanowires have a wide trap distribution, which leads to the abnormal thermal quenching behavior and persistent luminescence. Multifunctional AlN:Mn nanowires with magnetic and luminescence properties are expected to be used in spintronic and optoelectronic nanodevices.
Efficient power management unit for IIOT edge computing applications
Authors: Mukul Mukul Sharma , Shiv Shiv Kumar , Rashmi Rashmi Chawla 
Published: August 2021

Abstract
Applications of Industrial Internet of Things (IIoT) are comprehended in mass domains, and this reflects the prominence of IoT. However, the main con of IoT applications is that it requires continuous power to be drawn by various components to operate all the time. Energy harvesting provides a potential solution to this problem in many applications; however, this continual energy usage remains main IoT con. The energy generated by ambient sources is inadequate to run system application, and a need for better Power Management Unit (PMU) persists. This research paper proposes a PMU framework for energy efficiency in edge computing application with three layers of IoT architecture. Design-build on this deployment, constitutes sleep and awake scheduling, with calculative idle intervals. The transitions of states exercise an energy-efficient solution for the entire system. The design is tested on ZYBO Zynq-7000 (XC7Z010-1CLG400C) ARM/FPGA SoC board for development and evaluation. Simulation results demonstrate the task scheduling of our proposed architecture and energy consumption details. In brief, the proposed design proves to be a beneficial option, in particular, surpassing kin designs for industrial automation.
Rapid Visualization and Detection of Staphylococcus Aureus Based on Loop-Mediated Isothermal Amplification
Authors: Wu Chuan , Zeng Yuanyuan , He Yang 
Published: August 2021

Abstract
Staphylococcus aureus is a common clinical bacterial pathogen that can cause a diverse range of infections. The establishment of a rapid and reliable assay for the early diagnosis and detection of S. aureus is of great significance. In this study, we developed a closed-tube loop-mediated isothermal amplification (LAMP) assay for the visual detection of S. aureus using the colorimetric indicator hydroxy naphthol blue (HNB). The LAMP reaction was optimized by adjusting the amplification temperature, the concentrations of Mg2+, dNTP, and HNB, and the incubation time. In the optimized reaction system, the specificity of LAMP for S. aureus was 100%. The results established that this method accurately identified S. aureus, with no cross-reactivity with 16 non-S. aureus strains. The limit of detection (LOD) of LAMP was 8 copies/reaction of purified plasmid DNA or 400 colony-forming units/reaction of S. aureus. Compared with conventional PCR, LAMP lowered the LOD by 10-fold. Finally, 220 clinically isolated strains of S. aureus and 149 non-S. aureus strains were used to evaluate the diagnostic efficacy of LAMP. The findings indicated that LAMP is a reliable test for S. aureus and could be a promising tool for the rapid diagnosis of S. aureus infections.
Factors Contributing To The Swimmer Puppy Syndrome Found in Labrador Retrievers
Authors: TOMIHARI Mizuki , NOBUTOKI Yuko , NAKAJIMA Nagachika , YANAGAWA Masashi , TAGAWA Michihito , HAGIYA Koichi , NOMURA Tetsuro , SUWA Yoshinori , SUZUKI Hiroshi 
Published: August 2021

Abstract
Background
Swimmer puppy syndrome is a disease found in neonatal puppies mainly characterized by the inability to stand, but its direct cause is unknown. Since swimmer puppies were observed infrequently but continuously among the Labrador retriever colony at the Hokkaido Guide Dogs for the Blind Association in Japan, based on their birth record and pedigree, factors related to the onset of swimmer puppy syndrome in Labrador retrievers were examined.
Results
The total number of offspring over seven years was 436, of which 16 were swimmer puppies. Most of the affected puppies except one recovered steadily. As for the swimmer puppies, the litter size was significantly lower, and the body weights on the 10th and 28th day after delivery were significantly higher than the non-symptomatic puppies. These results suggested that the onset may be related to weight gain in the neonatal stages due to a small litter size. According to the genetic analysis, 26 ancestors common to the affected individuals were confirmed, but the causative individual could not be identified with the inbreeding coefficient. The heritability of the swimmer-puppy onset trait was 0.80, and the heritability for the the 10th -day body-weight trait was equally high at 0.78, both of which strongly suggest genetic involvement.
Conclusions
In this study, the onset of swimmer puppy syndrome in the Labrador retrievers was associated with litter size and early weight gain, and result of study suggests that genetic influence might be involved.
Effect of Inverted Internal Limiting Membrane Flap Technique on Normal Size Macular Holes
Authors: Yamada Kanako , Oishi Akio , Kusano Mao , Kinoshita Hirofumi , Tsuiki Eiko , Kitaoka Takashi 
Published: August 2021

Abstract
Inverted internal limiting membrane (ILM) flap technique was developed to achieve macular hole (MH) closure in large MH and refractory cases. In this study, we evaluate the effect of the technique for normal size MH. We recruited patients who underwent vitrectomy for normal size (< 400 µm) MH with either inverted ILM flap technique (flap group) or with conventional ILM peeling (peeling group). Using propensity score,21 eyes of 21 patients in the peeling group were matched against 21 eyes of 21 patients in the flap group. we compared MH closure rate, postoperative visual acuity, and recovery of the external limiting membrane (ELM) and ellipsoid zone (EZ). The MH closure rate was not different between the two groups (flap vs peeling: 90% vs 100%, P = 0.49). Whereas there was no significant difference in visual acuity improvement between the two groups, the flap group showed more disruption of the ELM 3 months after surgery and of the EZ at 3 and 6 months after surgery (P = 0.02, P = 0.03, and P = 0.04, respectively). The result suggested that inverted ILM flap technique does not have additional benefits for normal size MHs and may delay recovery of retinal integrity.