bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired Muscle Mitochondrial Function in Familial Partial Lipodystrophy
Authors: Simha Vinaya , Lanza Ian R , Dasari Surendra , Klaus Katherine A , Le Brasseur Nathan , Vuckovic Ivan , Laurenti Marcello C , Cobelli Claudio , Port John D , Nair K Sreekumaran 
Published: October 2021

Abstract

Context
Familial partial lipodystrophy (FPL), Dunnigan variety is characterized by skeletal muscle hypertrophy and insulin resistance besides fat loss from the extremities. The cause for the muscle hypertrophy and its functional consequences is not known.

Objective
To compare muscle strength and endurance, besides muscle protein synthesis rate between subjects with FPL and matched controls (n = 6 in each group). In addition, we studied skeletal muscle mitochondrial function and gene expression pattern to help understand the mechanisms for the observed differences.

Methods
Body composition by dual-energy X-ray absorptiometry, insulin sensitivity by minimal modelling, assessment of peak muscle strength and fatigue, skeletal muscle biopsy and calculation of muscle protein synthesis rate, mitochondrial respirometry, skeletal muscle transcriptome, proteome, and gene set enrichment analysis.

Results
Despite increased muscularity, FPL subjects did not demonstrate increased muscle strength but had earlier fatigue on chest press exercise. Decreased mitochondrial state 3 respiration in the presence of fatty acid substrate was noted, concurrent to elevated muscle lactate and decreased long-chain acylcarnitine. Based on gene transcriptome, there was significant downregulation of many critical metabolic pathways involved in mitochondrial biogenesis and function. Moreover, the overall pattern of gene expression was indicative of accelerated aging in FPL subjects. A lower muscle protein synthesis and downregulation of gene transcripts involved in muscle protein catabolism was observed.

Conclusion
Increased muscularity in FPL is not due to increased muscle protein synthesis and is likely due to reduced muscle protein degradation. Impaired mitochondrial function and altered gene expression likely explain the metabolic abnormalities and skeletal muscle dysfunction in FPL subjects.
Fetal Estrogens are not Involved in Sex Determination But Critical for Early Ovarian Differentiation in Rabbits
Authors: Jolivet Geneviève , Daniel-Carlier Nathalie , Harscoët Erwana , Airaud Eloïse , Dewaele Aurélie , Pierson Cloé , Giton Frank , Boulanger Laurent , Daniel Nathalie , Mandon-Pépin Béatrice , Pannetier Maëlle , Pailhoux Eric 
Published: October 2021

Abstract
AROMATASE is encoded by the CYP19A1 gene and is the cytochrome enzyme responsible for estrogen synthesis in vertebrates. In most mammals, a peak of CYP19A1 gene expression occurs in the fetal XX gonad when sexual differentiation is initiated. To elucidate the role of this peak, we produced 3 lines of TALEN genetically edited CYP19A1 knockout (KO) rabbits that were devoid of any estradiol production. All the KO XX rabbits developed as females with aberrantly small ovaries in adulthood, an almost empty reserve of primordial follicles, and very few large antrum follicles. Ovulation never occurred. Our histological, immunohistological, and transcriptomic analyses showed that the estradiol surge in the XX fetal rabbit gonad is not essential to its determination as an ovary, or for meiosis. However, it is mandatory for the high proliferation and differentiation of both somatic and germ cells, and consequently for establishment of the ovarian reserve.
Genetically altered animal models forATP1A3-related disorders
Authors: Ng Hannah W. Y. , Ogbeta Jennifer A. , Clapcote Steven J. 
Published: October 2021

ABSTRACTWithin the past 20 years, particularly with the advent of exome sequencing technologies, autosomal dominant and de novo mutations in the gene encoding the neurone-specific α3 subunit of the Na+,K+-ATPase (NKA α3) pump, ATP1A3, have been identified as the cause of a phenotypic continuum of rare neurological disorders. These allelic disorders of ATP1A3 include (in approximate order of severity/disability and onset in childhood development): polymicrogyria; alternating hemiplegia of childhood; cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss syndrome; relapsing encephalopathy with cerebellar ataxia; and rapid-onset dystonia-parkinsonism. Some patients present intermediate, atypical or combined phenotypes. As these disorders are currently difficult to treat, there is an unmet need for more effective therapies. The molecular mechanisms through which mutations in ATP1A3 result in a broad range of neurological symptoms are poorly understood. However, in vivo comparative studies using genetically altered model organisms can provide insight into the biological consequences of the disease-causing mutations in NKA α3. Herein, we review the existing mouse, zebrafish, Drosophila and Caenorhabditis elegans models used to study ATP1A3-related disorders, and discuss their potential contribution towards the understanding of disease mechanisms and development of novel therapeutics.
Development of Artificial Receptors Based on Assembly of Metal Complex Units and Desymmetrization of Molecular Components
Authors: Nakamura Takashi 
Published: October 2021

Abstract
This highlight review summarizes our recent studies of macrocyclic receptors with precise molecular recognition properties based on two concepts, assembly of metal complex units, and desymmetrization of molecular components. The metallomacrocycles realized a unique molecular binding by utilizing multipoint coordination and rigidity of the metal complex units. The desymmetrized homooligomeric macrocycles achieved a precise multipoint recognition by employing the unsymmetrically arranged interaction moieties.
Birch Reduction of Arenes Using Sodium Dispersion and DMI under Mild Conditions
Authors: Asako Sobi , Takahashi Ikko , Kurogi Takashi , Murakami Yoshiaki , Ilies Laurean , Takai Kazuhiko 
Published: October 2021

Abstract
An easy-to-handle sodium dispersion in paraffin oil (SD), in combination with inexpensive and environmentally benign 1,3-dimethyl-2-imidazolidinone (DMI) as an additive enables the Birch-type reduction of a variety of arenes with high yields, selectivity, and tolerance of functionality such as ether, alcohol, amine, amide, and carboxylic acid.
Can artificial intelligence replace ultrasound as a complementary tool to mammogram for the diagnosis of the breast cancer?
Authors: Mansour Sahar , Kamal Rasha , Hashem Lamiaa , AlKalaawy Basma 
Published: October 2021

Objective: To study the impact of artificial intelligence (AI) on the performance of mammogram with regard to the classification of the detected breast lesions in correlation to ultrasound-aided mammograms. Methods: Ethics committee approval was obtained in this prospective analysis. The study included 2000 mammograms. The mammograms were interpreted by the radiologists and breast ultrasound was performed for all cases. The Breast Imaging Reporting and Data System (BI-RADS) score was applied regarding the combined evaluation of the mammogram and the ultrasound modalities. Each breast side was individually assessed with the aid of AI scanning in the form of targeted heat-map and then, a probability of malignancy (abnormality scoring percentage) was obtained. Operative and the histopathology data were the standard of reference. Results: Normal assigned cases (BI-RADS 1) with no lesions were excluded from the statistical evaluation. The study included 538 benign and 642 malignant breast lesions (n = 1180, 59%). BI-RADS categories for the breast lesions with regard to the combined evaluation of the digital mammogram and ultrasound were assigned BI-RADS 2 (Benign) in 385 lesions with AI median value of the abnormality scoring percentage of 10 (n = 385/1180, 32.6%), and BI-RADS 5 (malignant) in 471, that had showed median percentage AI value of 88 (n = 471/1180, 39.9%). AI abnormality scoring of 59% yielded a sensitivity of 96.8% and specificity of 90.1% in the discrimination of the breast lesions detected on the included mammograms. Conclusion: AI could be considered as an optional primary reliable complementary tool to the digital mammogram for the evaluation of the breast lesions. The color hue and the abnormality scoring percentage presented a credible method for the detection and discrimination of breast cancer of near accuracy to the breast ultrasound. So consequently, AI- mammogram combination could be used as a one setting method to discriminate between cases that require further imaging or biopsy from those that need only time interval follows up. Advances in knowledge: Recently, the indulgence of AI in the work-up of breast cancer was concerned. AI noted as a screening strategy for the detection of breast cancer. In the current work, the performance of AI was studied with regard to the diagnosis not just the detection of breast cancer in the mammographic-detected breast lesions. The evaluation was concerned with AI as a possible complementary reading tool to mammogram and included the qualitative assessment of the color hue and the quantitative integration of the abnormality scoring percentage.
A digital territory to be appropriated: the state and the nationalization of cyberspace
Authors: Koulos Thanos 
Published: October 2021

Next to land, water, air and space, cyberspace is the complex socio-technical setting often called the ‘fifth domain’. Nationalism has taken over the organization of the first four domains, in the form of constructing national states, national territorial waters and national airspace. The basic proposition of this article is that the ideology of nationalism has also infiltrated the fifth domain – cyberspace – in two ways. First, through state-led cyber-nationalism via official government websites that present ‘national’ achievements and propagate the official state positions on disputes about territory, symbols or history. Second, through individual communities who use the internet to sustain a sense of national belonging and/or to promote and disseminate their nationalist ideals. Both ways are important in an online national identity (re)production framework that, in a fluid, global, modern world, functions supportively towards the traditional national identity (re)production mechanisms.This article aims to examine the patterns of the nationalization of cyberspace through an analysis of state-led institutions and government websites that aim to enhance national identity and the sense of national belonging in a globalized world, as well as to propagate official state positions. It will focus on Greek, Dutch, US and Israeli websites. The term ‘nationalization’ in this context denotes the ideological charging of the cyber-footprint of the nation: how the internet produces and re-produces the nation, how the users partake in the national community by way of ‘consuming’ the digitalized national ideology, and the way cyber-nationalism defines people’s sense of belonging.
Conjuring up the correspondence of high-school students’ self-determination and classroom climate with reading achievement
Authors: Joulide Shahrbanoo , Faravani Akram , Boori Ali Akbar 
Published: October 2021

A prevalent goal of education in schools is reading comprehension enhancement. Therefore, a critical issue in educational psychology is investigating the factors contributing to increase the reading achievement including both classroom climate and self-determination. This study explored the relationships of Iranian High School students’ self-determination and classroom climate with their reading achievement. 150 Iranian (male and female) students from Sama and Fazele high schools in Mashhad were selected through convenience sampling. The instruments were IOWA self-determination, Classroom Climate Questionnaires, and a validated researcher-made test. It was a type of quantitative and correlational research. Results revealed a significant and positive relationship between self-determination and reading ability. They also showed that among the six subscales of the self-determination, financial management had a significant relationship with the reading achievement scores of students. However, the emotional independence of peers had a negative correlation with the reading achievement scores of students. The results showed that teachers’ skill in orienting tasks can enhance reading achievement score and make the classroom climate more motivating. This study has potentially helpful implications for English language teachers, English institutes, and students. Teachers can enhance student’s self-determination by providing a friendly classroom environment and indirectly boost the students’ reading score.
Progress on the Starshot laser propulsion system
Authors: Worden S. Pete , Green Wesley A. , Schalkwyk James , Parkin Kevin , Fugate Robert Q. 
Published: October 2021

The Breakthrough Starshot research program envisions a laser-propelled sail that will probe our neighboring stars within a human lifetime. Starshot spacecraft weigh no more than a few grams and are accelerated by photon momentum transfer with a beam generated by a kilometer-scale, ground-based 100 GW coherent phased-array laser. We describe some of the major challenges for the laser and early exploratory efforts to overcome them. While challenges remain, we find no physical or economic restrictions that would preclude such a laser for the Starshot mission.
Bandwidth extension method based on the field-shunting effect in a high-gain photoelectric receiver circuit
Authors: Yang Jinqing , Wan Minjie , Qian Weixian , Ren Kan , Lu Dongming , Zhang Jun , Gu Guohua , Chen Qian 
Published: October 2021

In the high-gain photoelectric receiver circuit, the method based on the field-shunting effect is applied to improve the bandwidth of the transimpedance amplifier. This method is implemented by adding a ground trace under the gain resistor, which reduces the parasitic capacitance of the gain resistor and thus increases the bandwidth. To obtain the specific impact of this method on bandwidth, a series of simulations are carried out, including electromagnetic simulations of a three-dimensional structure of circuit gain part and simulation program with integrated circuit emphasis (SPICE) simulations of the high-gain voltage-current feedback transimpedance amplifier. Finally, the optimal simulation result shows that selecting a 1206 size chip fixed resistor and setting the ground trace width to 1.1 mm can greatly reduce the influence of resistor parasitic effects on the circuit, thereby achieving the best performance of bandwidth extension. Further, the comparative experiment also verifies the effectiveness of the method for bandwidth enhancement.
Predictive markers for the early prognosis of dengue severity: A systematic review and meta-analysis
Authors: Thach Tran Quang , Eisa Heba Gamal , Hmeda AlMotsim Ben , Faraj Hazem , Thuan Tieu Minh , Abdelrahman Manal Mahmoud , Awadallah Mario Gerges , Ha Nam Xuan , Noeske Michael , Abdul Aziz Jeza Muhamad , Nam Nguyen Hai , Nile Mohamed El , Dumre Shyam Prakash , Huy Nguyen Tien , Hirayama Kenji 
Published: October 2021

BackgroundPredictive markers represent a solution for the proactive management of severe dengue. Despite the low mortality rate resulting from severe cases, dengue requires constant examination and round-the-clock nursing care due to the unpredictable progression of complications, posing a burden on clinical triage and material resources. Accordingly, identifying markers that allow for predicting disease prognosis from the initial diagnosis is needed. Given the improved pathogenesis understanding, myriad candidates have been proposed to be associated with severe dengue progression. Thus, we aim to review the relationship between the available biomarkers and severe dengue.MethodologyWe performed a systematic review and meta-analysis to compare the differences in host data collected within 72 hours of fever onset amongst the different disease severity levels. We searched nine bibliographic databases without restrictive criteria of language and publication date. We assessed risk of bias and graded robustness of evidence using NHLBI quality assessments and GRADE, respectively. This study protocol is registered in PROSPERO (CRD42018104495).Principal findingsOf 4000 records found, 40 studies for qualitative synthesis, 19 for meta-analysis. We identified 108 host and viral markers collected within 72 hours of fever onset from 6160 laboratory-confirmed dengue cases, including hematopoietic parameters, biochemical substances, clinical symptoms, immune mediators, viral particles, and host genes. Overall, inconsistent case classifications explained substantial heterogeneity, and meta-analyses lacked statistical power. Still, moderate-certainty evidence indicated significantly lower platelet counts (SMD -0.65, 95% CI -0.97 to -0.32) and higher AST levels (SMD 0.87, 95% CI 0.36 to 1.38) in severe cases when compared to non-severe dengue during this time window.ConclusionThe findings suggest that alterations of platelet count and AST level—in the first 72 hours of fever onset—are independent markers predicting the development of severe dengue.
Association between Japanese community health workers’ willingness to continue service and two categories of motives: Altruistic and self-oriented
Authors: Taguchi Atsuko , Murayama Hiroshi , Ono Keiko 
Published: October 2021

BackgroundAs population aging progresses, volunteers in health field are expected to play a key role in health promotion and disease prevention, which may improve community residents’ health and well-being and at the same time help slow the growth of healthcare cost. The objective of this study is to examine the effects of self-oriented motives and altruistic motives as explanatory factors for Japanese Community Health Workers (CHWs)’ desire to continue their service. Unraveling the relative effects of these two types of motivation on CHW retention may lead to policy and practical implications for recruiting, training, and supporting CHWs in Japan. Haddad (2007) observed that citizens in Japan generally have a sense of governmental and individual responsibility for dealing with social problems. Applying these insights to CHWs, we hypothesize that altruistic motives have more potent influence on volunteers’ willingness to continue to serve than self-oriented motives.MethodsThree cities in Shiga prefecture, Japan agreed to participate in the study. Anonymous, self-administered questionnaire was mailed to all CHWs who work in the three communities. The survey data were collected in March and April, 2013. A total of 417 questionnaires were mailed to CHWs, of which 346 were completed and returned (response rate 83.0%). Nine questionnaires missing response to the question concerning willingness to continue serving were removed from the analysis. The final analysis used 337 questionnaires (effective response rate 80.8%).ResultsOne hundred ninety-nine (59.1%) of the respondents answered the question about willingness to continue CHW affirmatively, and 138 (40.9%) negatively. Controlling for other relevant factors, those with self-oriented motives in serving as CHWs were more likely to state they are willing to continue to serve (OR:1.54, confidence interval 1.00–2.37) than those without such motives. Those with altruistic motives were also more likely to say they want to continue their service (OR 1.56, confidence interval 1.08–2.27) than those without such motives. Contrary to our hypothesis, the two motives, altruistic and self-oriented, were shown to have nearly equal degree of influence on respondents’ willingness to continue serving as CHWs.ConclusionOne practical implication of the research is that learning more about the twin motives, self-oriented and altruistic, of volunteers and tailoring the content of CHW training by municipal health professionals to address those motives may be beneficial.
Towards an integrated model for supervision for mental health and psychosocial support in humanitarian emergencies: A qualitative study
Authors: Perera Camila , McBride Kelly A. , Travers Áine , Tingsted Blum Pia , Wiedemann Nana , Dinesen Cecilie , Bitanihirwe Byron , Vallières Frédérique 
Published: October 2021

Background
Despite recent advances in the development and provision of mental health and psychosocial support (MHPSS) in humanitarian settings, inadequate supervision remains a significant barrier to successful implementation. The present study sought to incorporate broad stakeholder engagement as part of the first phase of development of a new Integrated Model for Supervision (IMS) for use within MHPSS and protection services in humanitarian emergencies.

Methods
Semi-structured interviews were conducted with 26 global mental health professionals. Data was analysed thematically, using a combination of inductive and deductive methods. Codes and themes were validated through co-author cross-checks and through a webinar with an expert advisory group.

Results
Results reinforce the importance of effective supervision to enhance the quality of interventions and to protect supervisees’ wellbeing. Participants generally agreed that regular, supportive supervision on a one-to-one basis and as a separate system from line management, is the ideal format. The interviews highlight a need for guidance in specific areas, such as monitoring and evaluation, and navigating power imbalances in the supervisory relationship. Several approaches to supervision were described, including some solutions for use in low-resource situations, such as group, peer-to-peer or remote supervision.

Conclusion
An integrated model for supervision (IMS) should offer a unified framework encompassing a definition of supervision, consolidation of best practice, and goals and guidance for the supervisory process.