bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Mechanism of Danxiong Tongmai Granules in Treatment of Coronary Heart Disease
Authors: Ye Jiahao , Yang Ruiping , Hu Zhixi , Li Lin , Zhong Senjie , Jiang Ruixue 
Published: August 2021

Abstract

Background: Network pharmacology has been widely adopted for mechanistic studies of Traditional Chinese Medicines (TCM). The present study uses network pharmacology to investigate the main ingredients, targets and pathways of Danxiong Tongmai Granules (DXTMG) in the treatment of coronary heart disease (CHD). We aim to validate our findings using molecular docking and molecular dynamics simulations.Methods: TCM compounds and targets were identified via searches in the BATMAN-TCM database, and the GeneCards database were used to obtain the main target genes involved in CHD, We combined disease targets with the drug targets to identify common targets, and draw a Venn diagram to visualize the results. The "TCM-compound-target" network was plotted using Cytoscape 3.7.2 software and a protein-protein interaction (PPI) network was constructed using the STRING database from which core targets were obtained. Gene ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for common drug-disease targets using R Version 4.0.4 (64 bit) software. Molecular docking of core protein-small molecule ligand interaction was modeled using AutoDock Vina software. A simulation of molecular dynamics was conducted for the optimal protein-ligand complex obtained by molecular docking using Amber18 software.Results: 162 potential targets of DXTMG involved in CHD were identified. These included INS, ALB, IL-6 and TNF according to PPI network studies. GO enrichment analysis identified a total of 3365 GO pathways, including 3049 biological process pathways (BP) concerned with the heart and circulatory system;109 cellular component (CC) pathways, including cation channels and membrane rafts and 207 molecular function (MF) pathways related to receptor ligands and activators. KEGG analysis revealed a total of 137 pathways (p<0.05), including those related to AGE-RAGE signaling associated with diabetic complications, fluid shear stress and atherosclerosis. Molecular docking revealed the highest binding energy for Neocryptotanshinone Ii (the key compound of DXTMG) and TNF. Molecular dynamics simulation indicated stable binding for TNF-Neocryptotanshinone Ii with strong hydrophobic interactions mediated predominantly by the hydrophobic residues, Leu279, Val280 and Phe278 plus hydrogen-bonding with Leu279.Conclusion: The present study reveals novel insights into the mechanism of DXTMG in treating CHD. DXTMG can influence oxidative stress、inflammation response and regulating cardiomyocytes, thereby reducing the occurrence and development of CHD.
A Novel Approach to Estimate the Air Temperature Through the Air Pollution and Meteorological Conditions in Biskra Under the Parameters Astronomical, and Atmospheric
Authors: Chabane Foued , Arif Ali , Guettaf Abderrazak 
Published: August 2021

Abstract
Propose of the experimental study a found a sufficient model to predict the ambient temperature over the area of Biskra, Algeria, taking into account new air pollution factors such as CO, CO2, O3, and NO2. We found that the effect of air pollution, astronomical and meteorological elements have obvious in the ambient temperature, and be behavior, according to regional characteristics. Using the fitting of the experimental data for establishing a set of prediction data sets of pollutants, astronomical, and meteorological elements at the University of Biskra, Algeria. We recorded the highest NO2 value in March at an estimated value of 1.6.1015 (mole/cm), where the ambient temperature rate is low. The mathematical model obtained gives a satisfactory results, because they were substantially identical and without almost any error with the experimental data. Finally, the latter can be used by engineers and especially those who work in the environmental field which is very sensitive to temperature variation and it can influence the health of the human being.
Application of Ginsenoside-Rg1 Could Not Ameliorate The Hyperoxic-Induced Vascular Endothelial Oxidative Stress and Apoptosis
Authors: Fu Ren-Huei , Tseng Chi-Nan , Cho Yu-Hsueh , Chu Yen 
Published: August 2021

Abstract
Background: Oxygen therapy is necessary to preterm infants with respiratory distress. However, hyperoxia may cause bronchopulmonary dysplasia and retinopathy of prematurity due to suppression of vasogenesis and increase of cell death. Ginsenoside-Rg1, one of the active components of Ginsen, is shown as a proangiogenic factor of vascular endothelial cells. We evaluated whether application of Ginsenoside-Rg1 is able to improve hyperoxic-induced vascular endothelium injury.Materials and methods: Human umbilical vein endothelial cells (HUVECs) were cultured under room air and 60% oxygen for 72 hours, respectively. Gensenoside-Rg1 was added to the medium at 0, 75, 150, 300nM. HUVECs proliferation, oxidative stress and apoptosis under normoxic and hyperoxic conditions were assayed by Western Blot.Results: Under hyperoxia (60% O2), HUVECs proliferation and levels of vascular endothelial growth factor (VEGF) were significantly decreased after ginsenoside-Rg1 treatment. Interestingly, both levels of glucocorticoid receptor (GR) and glutathione peroxidase (GPx) were increased after 72 hr Ginsenoside-Rg1 treatment, but no changed under room air control. The levels of oxidative stress-induced Bax and cytochrome c and apoptosis-related active caspase 3 and poly ADP-ribose polymerase were significantly increased after ginsenoside-Rg1 treatment under hyperoxic condition.Discussion and conclusion: In HUVECs model, Ginsenoside-Rg1 is unable to overcome the major hyperoxic-induced vascular endothelium injury. It might aggravate oxidative stress and endothelial apoptosis caused by oxygen toxicity. However, both elevated levels of GR and GPx indicate that Ginsenoside-Rg1 could be involved in vascular signaling and the regulation of oxidative stress under hyperoxia. Further investigation of Rg1 effects under hyperoxia is required.
Synthesis, Molecular Docking, And Antitumor Evaluation of Some New Pyrazole, Pyridine, And Thiazole Derivatives Incorporating Sulfonamide Residue
Authors: Elgogary Sameh , Radini Ibrahim 
Published: August 2021

Abstract
Cancer is the second leading cause of death worldwide. There is always a huge demand for novel anticancer. New series of pyrazole, pyridine, thiazole derivatives containing sulfonamide moiety were prepared and screened for their antitumor activity against breast cancer cell line (MCF-7). The results of this investigation revealed that compounds 3 and 8 had significant anticancer activity against the MCF-7 cancer cell line with IC50 values 19.2 and 14.2 μM, respectively, in relation to the standard drug, doxorubicin.
CD14 and LBP as Novel Biomarkers for Sarcoidosis by Proteomics of Extracellular Vesicles
Authors: Futami Yu , Takeda Yoshito , Koba Taro , Narumi Ryohei , Nojima Yosui , Ito Mari , Nakayama Mana , Ishida Mimiko , Yoshimura Hanako , Naito Yujiro , Fukushima Kiyoharu , Takimoto Takayuki , Edahiro Ryuya , Matsuki Tomonori , Nojima Satoshi , Hirata Haruhiko , Koyama Shohei , Iwahori Kota , Nagatomo Izumi , Shirai Yuya , Suga Yasuhiko , Satoh Shingo , Futami Shinji , Miyake Kotaro , Shiroyama Takayuki , Inoue Yoshikazu , Adachi Jun , Tomonaga Takeshi , Ueda Koji , Kumanogoh Atsushi 
Published: August 2021

Abstract
Sarcoidosis is an inflammatory granulomatous multi-organ disease of unknown cause. The granulomatous inflammation in sarcoidosis is driven by the interplay between T cells and macrophages. Extracellular vesicles (EVs) play important roles in intercellular communication. Isolated serum EVs from seven patients with sarcoidosis and five control subjects were subjected to non-targeted proteomics analysis. Then 2,292 proteins were detected; 42 proteins were upregulated in patients with sarcoidosis relative to control subjects, and 324 proteins were downregulated. The protein signature of EVs from patients with sarcoidosis reflected disease characteristics such as antigen presentation and immunological disease. Candidate biomarkers were further verified by targeted proteomics analysis in 46 patients and 10 control subjects. CD14 and lipopolysaccharide-binding protein (LBP) were validated by targeted proteomics analysis and upregulation of them was confirmed by immunoblotting. Their expression was also upregulated in macrophages of lung granulomatous lesions. Consistent with these findings, CD14 levels were increased in lipopolysaccharide-stimulated macrophages during multinucleation, concomitant with increased levels of CD14 and LBP in EVs. The area under the curve values of CD14 and LBP were 0.81 and 0.84, respectively. Thus CD14 and LBP in serum EVs, which are associated with granulomatous pathogenesis, can improve the diagnostic accuracy in patients with sarcoidosis.
Identification of Differential Gene Expression and Related Signaling Pathways In SARS-COV-2 Infected Human Cells
Authors: Xie Tian-Ao , Li Hou-He , Lin Zu-En , Lin Xiao-Ye , Meng Xin , Zhong Qi , Guo Xuguang 
Published: August 2021

Abstract
Background: The Corona Virus Disease 2019 (COVID-19) pandemic poses a serious public health threat to the survival and health of people all over the world. We analyzed related mRNA data and gene expression profiles of human cell lines infected with SARS-CoV-2 obtained from GEO (GSE148729), using bioinformatics tools. Differentially expressed genes (DEGs) of human cells infected with SARS-CoV-2 were identified.Method: The GSE148729 datasets were downloaded from the Gene Expression Omnibus (GEO) database. To explore the Biological significance of DEGs, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of the DEGs was performed. Protein-protein interaction (PPI) networks of the DEGs were constructed by using the STRING database. The hub genes were selected using the Cytoscape Software, and a t-test was performed to validate the hub genes.Result: A total of 1241 DEGs were screened, including 1049 up-regulated genes and 192 down-regulated genes. Besides, 10 hub genes were obtained from the PPI network, among which the expression level of CXCL2, Etv7, and HIST1H2BG was found to be statistically significant.Conclusion: In conclusion, bioinformatics analysis reveals genes and cellular pathways that are significantly altered in SARS-CoV-2 infected cells. This is conducive to further guide the clinical study of SARS-CoV-2 and provides new perspectives for vaccine development.
Enhanced glyoxylate shunt for efficient production of C4 derivatives using fatty acids feedstocks
Authors: Miao Yingchun , liu Jiao , wang Xuanlin , Liu Bo , Liu Weifeng , Tao Yong 
Published: August 2021

Abstract
Biosynthesis of TCA cycle-derived C4 chemicals through glyoxylate shunt is an attractive metabolic route because it can be drived by TCA-glyoxylate cycle force under aerobic conditions. However, yield of this route is low with at least 1/3 carbon loss from glucose. FAs could sufficiently provide acetyl-CoA by β-oxidation without carbon loss and directly enter the TCA-glyoxylate cycle, which is acknowledged as a promising alternative feedstock. Here β-alanine was selected as the target TCA cycle-derived chemical, of which the theoretical yield is 1.391 g/g FAs, much higher than that of glucose(0.49 g/g). By adopting multi-metabolic engineering strategies and relieving the active oxygen damage caused by FAs utilization, β-alanine production reached 78.05 g/L with a yield of 1.2 g/g, about 86% of theoretical yield. Our study establish a promising bioproduction route of β-alanine from waste FAs (such as gutter oil, palm fatty acid distillate etc.), and more importantly, provide an efficient platform for TCA cycle-derived C4 chemicals biosynthesis.
Epidemiological Comparison of Osteoporotic Hip Fractures in Spain and China: A Multicenter Study
Authors: Gao Shuai-Shuai , Capitan-Morales Luis C , Wang Yan-Jun , Yao Yong-Feng , Zhang Ya-Hui , Verdera Aurelio Borrás , Zhang Wen-Ting 
Published: August 2021

Abstract
Objective Compare the epidemiological differences of hip fracture patients in Spain and China. Materials and Method This was a retrospective study. The study population came from patients with hip fractures who underwent surgery between January 1, 2017 and December 30, 2017 at the University Hospital Virgen Macarena (UHVM) in Seville, Spain and Xi’an Daxing Hospital (XDH) in Shaanxi Province, China. Through medical history review and telephone follow-up, the differences of demographic and anthropometric characteristics, lifestyle, personal medical history, variables related to injury, anesthesia, and surgery, length of stay in hospital, days of bed rest, and mortality of patients in the two hospitals were compared. Results A total of 757 patients were enrolled, of which 426 were from UHVM and 331 were from XDH. The average age was 81.4±9.26 years and 76.0±8.08 years, respectively; the male and female distribution was 25.1% and 74.9% vs. 32.0% and 68.0%. The average BMI of Spanish patients was higher than that of Chinese patients. The proportion of drinking and smoking among Spanish patients was significantly higher than that of Chinese patients (p = 0.000, p = 0.000, respectively). The proportion of patients with ≥ 3 comorbidities in Spain was higher than that in China (p = 0.002). The surgery delay time of Spanish patients was significantly longer than that of Chinese patients (p = 0.000). Spanish patients had a shorter hospital stay, but a longer time in bed. During the one-year follow-up, there were 81 deaths in Spain and 43 deaths in China (p = 0.026). Conclusiones There are large epidemiological differences between hip fracture patients in Seville, Spain and Xi'an, China in terms of demographics, lifestyle, comorbidities, surgical methods, and mortality.
On the Greenspan Resonance of Meteotsunamis in the Yellow Sea - Insights from the Newly Discovered 2009 Event
Authors: Kim Jihwan , Choi Byoung-Ju , Omira Rachid 
Published: August 2021

Abstract
The Yellow Sea is recognized as a meteotsunami “hot-spot”, with a relatively high rate of events’ occurrence. The March 2007 and May 2008 meteotsunami events attracted large attention due to their deadly and high impact on the west coast of the Korean Peninsula. However, other small size meteotsunamis remain less known because of their insignificant coastal effect. Yet, a better understanding of meteotsunami hazard in the Yellow Sea requires thorough investigation of both large and small events. This paper reveals the occurrence of a meteotsunami on 11–12 June 2009 in the eastern Yellow Sea. It addresses the analyses of both the recorded sea-level and air-pressure data, the correlation between the atmospheric forcing and the meteotsunami formation, the numerical modeling of meteotsunami propagation, and the resonance effects on the recorded waves. Analysis results evidence a moving air-pressure jump of about 3 hPa that disturbed the sea surface and caused a meteotsunami with wave height up to 0.45 m (crest-to-trough). Both meteorological observations and numerical modeling suggest a speed of 11 to 13 m/s for the atmospheric disturbance propagation, which is much smaller than the optimal condition for Proudman resonance of meteotsunamis in the eastern Yellow Sea. Here, we demonstrate that the Greenspan resonance was responsible for amplifying the incident waves. Despite the insignificant coastal impact of the 11 June 2009 event, its investigation unravels new insights into the formation, amplification, and hazard extent of small size meteotsunamis in the Yellow Sea.
The Effects And Possible Mechanisms of Vortioxetine on Pentylenetetrazole-Induced Seizures In Rats
Authors: Taskiran Ahmet Sevki , Filiz Ahmet Kemal 
Published: August 2021

Abstract
Antidepressants are known to demonstrate various effects on the nervous system. As a new antidepressant, vortioxetine is used for major depression in adult patients, with no clear indication of epileptic seizures. Therefore, the aim here was to examine the impact of vortioxetine on pentylenetetrazole-induced epileptic seizures in rats. The rats were randomly divided into 5 groups, each with 6 rats. Group 1 was control, Group 2 was administered saline (1 mL/kg/day serum physiologic), Group 3 was given (1 mg/kg/day diazepam), and Groups 4 and 5 were administered vortioxetine (2.5 and 5 mg/kg/day). The experimental groups (Groups 2-5) were given the drugs for a total of 7 days. Pentylenetetrazole (45 mg/kg) was administered on day 7 to all but the control group. Behavioral testing was performed using the passive avoidance and open field tasks. Total antioxidant status (TAS), total oxidant status (TOS), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1 β), neuronal nitric oxide synthase (nNOS), nitric oxide (NO), soluble guanylate cyclase (sGC), cyclic guanosine monophosphate (cGMP) caspase-3, and caspase-9 levels were measured using a commercial kit. The groups receiving vortioxetine (2.5 mg/kg and 5 mg/kg) were found to have delayed epileptic seizure onset times and reduced seizure stages with improved memory impairment after seizures. These groups also had increased TAS levels and decreased TOS levels in the cortex and hippocampus. Additionally, TNF-α, IL-1 β, nNOS, sGC, cGMP, caspase-3, and caspase-9 levels in the cortex and hippocampus were statistically significantly lower for these groups. Vortioxetine was determined to have protective effects on pentylenetetrazole-induced seizures in rats, with alleviated seizures and improved memory impairment, oxidative stress, inflammation, and apoptosis. The mechanisms of vortioxetine may involve the inhibition of oxidative stress, inflammation, and the nNOS/sGC/cGMP signalling pathway.
Modified Levator Muscle Complex Suspension on Treating Pediatric Blepharoptosis with Poor Bell’s Phenomenon
Authors: Li Rui , Zhu Xiao-Wei , Zhang Jia-Ying , Ding Xia , Wu Xu-Sheng , Xing Yue , Lin Ming , Li Jin 
Published: August 2021

Abstract
Purpose: To evaluate the surgical outcomes of pediatric congenital blepharoptosis with poor Bell’s phenomenon (BP) treated with modified levator muscle complex suspension.Methods: Forty-two pediatric congenital blepharoptosis patients with poor BP were treated with modified levator muscle complex suspension, and their major surgical outcomes such as marginal reflex distance1 (MRD1), palpebral fissure height (PFH), and postoperative lagophthalmos were retrospectively reviewed. Results: The mean follow-up was 10.28±9.89 months (range: 3-32 Months). Surgical success was achieved in 54 (87.1%) of 62 eyelids at the final visit, including excellent results in 46 (74.2%) eyelids, good results in 8 (12.9%) eyelids, and poor results in 8 (12.9%) eyelids, respectively. The postoperative PFH of affected eyes (7.97±1.47 mm) was significantly improved compared with that before surgery (3.58±1.31 mm). The mean MRD1 was improved from -1.48±1.36 mm before surgery to 2.94±1.46 mm after surgery. The postoperative MRD1 was ≥ 3 mm in 46 eyelids and < 3 mm in 16 eyelids. The mean lagophthalmos was 1.42±1.20 mm 3 months after surgery. All of the patients presented complete blink postoperatively. Postoperative complications were rarely observed during follow-up. No patient had exposure keratitis, but blepharoptosis recurred in 6 patients (8 eyelids). All patients had satisfactory eyelid symmetry and contour. No complications were observed until the last visit.Conclusions: The modified method results complete blink, mild and quick recovery of lagophthalmos, flexible eyelid motility, stable ocular surface, and it is simple to perform with few complications and a low recurrence rate at 12.9%, which is worth to wide application on poor bell’s phenomenon blepharoptosis.
ACE2 Expression in Lungs of Severe COVID-19 Infection: A Study on Minimally Invasive Post- mortem Tissue Samples
Authors: Gheware Atish , Ray Animesh , Rana Deeksha , Bajpai Prashant , Nambirajan Aruna , Arulselvi S , Mathur Purva , Trikha Anjan , Arava Sudheer , Das Prasenjit , Mridha Asit Ranjan , Singh Geetika , Soneja Manish , Nischal Neeraj , Lalwani Sanjeev , Wig Naveet , Sarkar Chitra , Jain Deepali 
Published: August 2021

Abstract
Angiotensin-converting enzyme 2 (ACE2) is a key host protein by which severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) enters and multiplies within cells. The level of ACE2 expression in the lung is hypothesised to correlate with an increased risk of severe infection and complications in COVID-19 (COrona VIrus Disease 2019). To test this hypothesis, we compared the protein expression status of ACE2 by immunohistochemistry (IHC) in post-mortem lung samples of patients who died of severe COVID-19 and lung samples obtained from non-COVID-9 patients for other indications. IHC for CD61 and CD163 were performed for assessment of platelet-rich microthrombi and macrophages, respectively. IHC for SARS-CoV-2 viral antigen was also performed. Quantification of immunostaining, random sampling, and correlation analysis was used to substantiate the morphologic findings. Our results show that among a total of 44 COVID-19 post-mortem lung tissues and 15 lung biopsies in non-COVID-19 patients included, ACE2 protein expression was significantly higher in COVID-19 patients than in controls, regardless of sample size. Histomorphology in COVID-19 lungs showed diffuse alveolar damage (DAD), acute bronchopneumonia, and acute lung injury with SARS-CoV-2 viral protein detected in a subset of cases. ACE2 expression levels positively correlated with increased expression levels of CD61 and CD163. In conclusion, our results show significantly higher ACE2 protein expression in severe COVID-19 disease, correlating with increased macrophage infiltration and microthrombi, suggesting a pathobiological role in disease severity.
Study of natural aging on nanoparticles and their strengthening effect in Al-Mg-Si alloy
Authors: Cui Zhenjie , Jiang Haichang , Zhang Duo , Song Yuanyuan , Yan Desheng , Rong Lijian 
Published: August 2021

Abstract
Atom probe tomography (APT) and transmission electron microscopy (TEM) were employed simultaneously to study nanoparticles and their strengthening effect in artificial aging (AA) Al-Mg-Si alloy with different natural aging (NA). We discovered that NA leads to formation of different type of nanoparticles meanwhile affect their fraction and dimension. Separation of the contribution from different types of nanoparticles to the alloy strength is conducted. In LNA-AA, β′ with a volume fraction of 0.16% contributions 54MPa to the yield strength while 0.14% β″ contributes 101MPa, which fully demonstrates that β″ is the main strengthening phase. The thermodynamic analysis was conducted for further research and the differential scanning calorimeter (DSC) results show that LNA forms more numerous clusters Ⅰ than SNA and consumes more vacancies and solute atoms then does not have enough driving force to precipitate clusters Ⅱ which are the nucleation sites of nanoparticles. When there is a certain distance between the nucleation sites, preferential growth of few β″ occur then converted to β′. The solute atoms in the region nearly which there is no nucleation site do not have enough driving force to diffuse and precipitate but stay in the matrix, reducing the volume fraction of the nanoparticles.