bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-stage total knee arthroplasty and femoral osteotomy for osteoarthritis with severe supracondylar deformity
Authors: Sun Jing-yang , Zhang Guo-qiang , Shen Jun-min , Du Yin-qiao , Li Tie-jian , Geng Zong-jie , Zhou Yong-gang , Wang Yan 
Published: February 2021

AbstractBackgroundKnee osteoarthritis associated with extra-articular deformity (EAD) can confront the arthroplasty surgeons with challenges of bone resection and soft tissue balancing. The aim of this study was to describe a single-stage procedure associating corrective osteotomy with total knee arthroplasty (TKA), and to determine the outcome at mid- to long-term follow-up.MethodsA total of seven patients (seven knees) with knee osteoarthritis and supracondylar deformity were included in this study. Six patients were female, and one was male, with the median age of 62 years (range, 37-76 years). All patients were treated with single-stage TKA and femoral osteotomy. Osteotomy was fixed with long cemented stem. Hospital of Special Surgery (HSS) scores, collateral ligament laxity, and range of motion (ROM) were clinically evaluated preoperatively and at each follow-up. Radiographic parameters including the mechanical axis deviation (MAD), mechanical lateral distal femoral angle (mLDFA), mechanical proximal tibial angle (mMPTA), and joint line congruence angle (JLCA) were also measured. The occurrence of perioperative complications was recorded.ResultsThe median follow-up time was 91 months (range, 38-104 months). At the last follow-up, all components were stable and no patients required revision. Nonunion of the osteotomy occurred in one patient. In all patients, the lower limb mechanical alignment improved greatly. The mean angle of MAD was restored from 10.49±6.05 cm preoperatively to 1.11±4.97 cm postoperatively. The 90° mLDFA was almost acquired in all cases, with the postoperative value of 90.79±2.40°. After operation, the mMPTA improved from 84.18±6.13° to 91.33±3.13°. The JLCA changed from 2.94±1.61° to −0.71±3.50°. The median HSS score improved from 45 (range, 34-56) preoperatively to 90 (range, 82-97) postoperatively, with the outcome of all patients rated good to excellent. The median ROM improved from 70° (range 0–110°) preoperatively to 105° (range 90–125°) postoperatively. No instability of knee joint was observed. Complications included an intraoperative split fracture of distal femur and one case of wound exudation resulting from fat liquefaction.ConclusionsFor knee osteoarthritis with femoral supracondylar deformity, single-stage TKA and corrective osteotomy was feasible but technically demanding. The use of long cemented stem for osteotomy fixation can provide reliable rotational control of the bone segments.
One-year outcome of manualised behavior therapy of chronic tic disorders in children and adolescents
Authors: Nissen J. B. , Carlsen A. H. , Thomsen P. H. 
Published: February 2021

Abstract
Background
Chronic tic disorders are neurodevelopmental disorders that can be treated with Habit Reversal Training (HRT) and Exposure Response Prevention (ERP). Intermediate and long-term effects have been examined after individual treatment with HRT, whereas evaluation of long-term outcome after an initial treatment with ERP, or a combination of HRT and ERP is lacking.
The present study examines the long-term effect after a combined treatment with HRT and ERP delivered in an individual or a group setting

Methods
Fifty-nine children and adolescents diagnosed with a chronic tic disorder were randomised to manualised treatment combining HRT and ERP as individual or group training. Forty-seven were re-examined 1 year after acute outcome. Outcome measures included Total Tic Severity score (TTS) measured by the Yale Global Tic Severity Scale (YGTSS) and Beliefs About Tics Scale (BATS)

Results
In a mixed model, it was shown that the initial improvement with both individual and group treatment was maintained throughout the follow-up period. There were no significant differences between the two methods of treatment delivery. Of all participants completing the 12 months evaluation, 74.4% were considered responders. There was a significant positive association between the reduction of TTS and the reduction in BATS. In a latent class post-treatment trajectory analysis, two classes were identified, where high baseline severity increased the likelihood of being in the lesser responder class. Similar, but only as a trend, having ADHD, planning difficulties or hypersensitivity increased the risk of a lesser response.

Conclusions
The present study compares the efficacy in individualised and group treatment of providing manualised therapy for child and adolescent tic disorders using two behavioural methods (combined HRT and ERP) both of which have been shown to have acute benefits but only one of which has been validated for longer term effectiveness. In the present study, both individualised and group treatments showed benefit throughout a 1-year follow-up period with several potential confounds affecting outcomes, while the relative benefits of either HRT and ERP were not addressed.
Trial registration NCT04594044, 1-10-72-216-15, registered 19th October 2020, retrospectively registered, https://register.clinicaltrials.gov/prs/app/template/Home.vm?uid=U0005BW2&ts=9&sid=S000ABEY&cx=-wlx7vb
The study is approved by the National Ethical Committee (1-10-72-216-15) and the Danish Data Protection Agency (1–16-02-490-15), registered 12 October 2015.
The risk of postoperative deterioration of non-cardiac surgery patients with ICU referral status who are admitted to the regular ward: a retrospective observational cohort study
Authors: Moore David , Durie Matthew L. , Bampoe Sohail , Buizen Luke , Darvall Jai N. 
Published: February 2021

Abstract
Background
Higher-risk surgical patients may not be admitted to the intensive care unit due to stable immediate post-operative status on review. The outcomes of this cohort are not well described. Our aim was to examine the subsequent inpatient course of intensive care unit -referred but not admitted surgical patients.

Methods
All patients aged ≥18 years who were referred but not admitted for post-operative management in a tertiary metropolitan intensive care unit following non-cardiac surgery between 1/7/2017 and 30/6/2018 were eligible for inclusion in this retrospective observational cohort study. Primary outcome was Medical Emergency Team activation. Secondary outcomes included unplanned intensive care unit admission; length of stay; and 30-day mortality. Risk of serious complications and predicted length of stay were calculated using the National Surgical Quality Improvement Program scoring tool.

Results
Fifteen of 60 patients (25%) had a MET-call following surgery, eight (13%) patients required unplanned intensive care unit admission, with median (IQR) time to Medical Emergency Team call 9 (6–13) hours. No patients died within 30-days. There was no significant difference between mean National Surgical Quality Improvement Program predicted and actual length of stay; after adjustment, National Surgical Quality Improvement Program predicted risk of serious complications was associated with unplanned intensive care unit admission (OR [95% CI] = 1.08 [1.00–1.16], p = 0.04), although not Medical Emergency Team calls.

Conclusions
Post-operative deterioration occurs frequently, and early, in a cohort of high-risk surgical patients initially assessed as being safe for ward care. Changes to current triage models for post-operative intensive care unit admission may reduce the impact of complications in this high-risk group.
Genomic properties of variably methylated retrotransposons in mouse
Authors: Elmer Jessica L. , Hay Amir D. , Kessler Noah J. , Bertozzi Tessa M. , Ainscough Eve A. C. , Ferguson-Smith Anne C. 
Published: February 2021

AbstractBackgroundTransposable elements (TEs) are enriched in cytosine methylation, preventing their mobility within the genome. We previously identified a genome-wide repertoire of candidate intracisternal A particle (IAP) TEs in mice that exhibit inter-individual variability in this methylation (VM-IAPs) with implications for genome function.ResultsHere we validate these metastable epialleles and discover a novel class that exhibit tissue specificity (tsVM-IAPs) in addition to those with uniform methylation in all tissues (constitutive- or cVM-IAPs); both types have the potential to regulate genes incis. Screening for variable methylation at other TEs shows that this phenomenon is largely limited to IAPs, which are amongst the youngest and most active endogenous retroviruses. We identify sequences enriched within cVM-IAPs, but determine that these are not sufficient to confer epigenetic variability. CTCF is enriched at VM-IAPs with binding inversely correlated with DNA methylation. We uncover dynamic physical interactions between cVM-IAPs with low methylation ranges and other genomic loci, suggesting that VM-IAPs have the potential for long-range regulation.ConclusionOur findings indicate that a recently evolved interplay between genetic sequence, CTCF binding, and DNA methylation at young TEs can result in inter-individual variability in transcriptional outcomes with implications for phenotypic variation.
Breast tuberculosis: a case series
Authors: Ghalleb M. , Seghaier S. , Adouni O. , Bouaziz H. , Bouida A. , Hassouna J. Ben , Chargui R. , Rahal K. 
Published: February 2021

Abstract
Background and aim
Breast tuberculosis is a rare disease, even in endemic areas. The diagnosis can be challenging, as it can mimic breast cancer. We aim to report our experience and discuss diagnoses and management modalities.

Results
We encountered twelve cases of breast tuberculosis in our institution from 2004 to 2019. The average age of our Caucasian North African patients was 42 years old (22–63). The classic presentation was a breast lump found in half of the cases. On physical examination, we suspected breast carcinoma in seven patients. The average size of the tumors was 39 mm (15–80 mm). Nine patients had a mammogram. In five cases, there was a suspicious breast mass mimicking a malignant tumor with an average size of 33 mm (25–60 mm). A ultrasonography was performed in 6 cases and revealed a suspicious ill-circumscribed nodule in four patients with an average size of 37.5 mm (10–60 mm). Five patients had a lumpectomy, and seven women underwent drainage of the abscess and the biopsy of its hull. The association of epithelioid cell granulomas and caseous necrosis was mandatory for the histological diagnosis of tuberculosis. All of them had an antitubercular therapy. The median period of follow-up was of 43 months (3–156 months). One patient presented with a recurrent abscess of the breast.

Conclusion
Our study found that clinical examination and radiological imaging were not specific. Positive cultures for Koch bacillus or histological confirmation are mandatory for the diagnosis. A meta-analysis of the existing cases is needed.
Next-generation diagnostics: virus capture facilitates a sensitive viral diagnosis for epizootic and zoonotic pathogens including SARS-CoV-2
Authors: Wylezich Claudia , Calvelage Sten , Schlottau Kore , Ziegler Ute , Pohlmann Anne , Höper Dirk , Beer Martin 
Published: February 2021

Abstract
Background
The detection of pathogens in clinical and environmental samples using high-throughput sequencing (HTS) is often hampered by large amounts of background information, which is especially true for viruses with small genomes. Enormous sequencing depth can be necessary to compile sufficient information for identification of a certain pathogen. Generic HTS combining with in-solution capture enrichment can markedly increase the sensitivity for virus detection in complex diagnostic samples.

Methods
A virus panel based on the principle of biotinylated RNA baits was developed for specific capture enrichment of epizootic and zoonotic viruses (VirBaits). The VirBaits set was supplemented by a SARS-CoV-2 predesigned bait set for testing recent SARS-CoV-2-positive samples. Libraries generated from complex samples were sequenced via generic HTS (without enrichment) and afterwards enriched with the VirBaits set. For validation, an internal proficiency test for emerging epizootic and zoonotic viruses (African swine fever virus, Ebolavirus, Marburgvirus, Nipah henipavirus, Rift Valley fever virus) was conducted.

Results
The VirBaits set consists of 177,471 RNA baits (80-mer) based on about 18,800 complete viral genomes targeting 35 epizootic and zoonotic viruses. In all tested samples, viruses with both DNA and RNA genomes were clearly enriched ranging from about 10-fold to 10,000-fold for viruses including distantly related viruses with at least 72% overall identity to viruses represented in the bait set. Viruses showing a lower overall identity (38% and 46%) to them were not enriched but could nonetheless be detected based on capturing conserved genome regions. The internal proficiency test supports the improved virus detection using the combination of HTS plus targeted enrichment but also points to the risk of cross-contamination between samples.

Conclusions
The VirBaits approach showed a high diagnostic performance, also for distantly related viruses. The bait set is modular and expandable according to the favored diagnostics, health sector, or research question. The risk of cross-contamination needs to be taken into consideration. The application of the RNA-baits principle turned out to be user friendly, and even non-experts can easily use the VirBaits workflow. The rapid extension of the established VirBaits set adapted to actual outbreak events is possible as shown for SARS-CoV-2.
A gene co-association network regulating gut microbial communities in a Duroc pig population
Authors: Reverter Antonio , Ballester Maria , Alexandre Pamela A. , Mármol-Sánchez Emilio , Dalmau Antoni , Quintanilla Raquel , Ramayo-Caldas Yuliaxis 
Published: February 2021

Abstract
Background
Analyses of gut microbiome composition in livestock species have shown its potential to contribute to the regulation of complex phenotypes. However, little is known about the host genetic control over the gut microbial communities. In pigs, previous studies are based on classical “single-gene-single-trait” approaches and have evaluated the role of host genome controlling gut prokaryote and eukaryote communities separately.

Results
In order to determine the ability of the host genome to control the diversity and composition of microbial communities in healthy pigs, we undertook genome-wide association studies (GWAS) for 39 microbial phenotypes that included 2 diversity indexes, and the relative abundance of 31 bacterial and six commensal protist genera in 390 pigs genotyped for 70 K SNPs. The GWAS results were processed through a 3-step analytical pipeline comprised of (1) association weight matrix; (2) regulatory impact factor; and (3) partial correlation and information theory. The inferred gene regulatory network comprised 3561 genes (within a 5 kb distance from a relevant SNP–P < 0.05) and 738,913 connections (SNP-to-SNP co-associations). Our findings highlight the complexity and polygenic nature of the pig gut microbial ecosystem. Prominent within the network were 5 regulators, PRDM15, STAT1, ssc-mir-371, SOX9 and RUNX2 which gathered 942, 607, 588, 284 and 273 connections, respectively. PRDM15 modulates the transcription of upstream regulators of WNT and MAPK-ERK signaling to safeguard naive pluripotency and regulates the production of Th1- and Th2-type immune response. The signal transducer STAT1 has long been associated with immune processes and was recently identified as a potential regulator of vaccine response to porcine reproductive and respiratory syndrome. The list of regulators was enriched for immune-related pathways, and the list of predicted targets includes candidate genes previously reported as associated with microbiota profile in pigs, mice and human, such as SLIT3, SLC39A8, NOS1, IL1R2, DAB1, TOX3, SPP1, THSD7B, ELF2, PIANP, A2ML1, and IFNAR1. Moreover, we show the existence of host-genetic variants jointly associated with the relative abundance of butyrate producer bacteria and host performance.

Conclusions
Taken together, our results identified regulators, candidate genes, and mechanisms linked with microbiome modulation by the host. They further highlight the value of the proposed analytical pipeline to exploit pleiotropy and the crosstalk between bacteria and protists as significant contributors to host-microbiome interactions and identify genetic markers and candidate genes that can be incorporated in breeding program to improve host-performance and microbial traits.
A flexible univariate moving average time-series model for dispersed count data
Authors: Sellers Kimberly F. , Arab Ali , Melville Sean , Cui Fanyu 
Published: February 2021

AbstractAl-Osh and Alzaid (1988) consider a Poisson moving average (PMA) model to describe the relation among integer-valued time series data; this model, however, is constrained by the underlying equi-dispersion assumption for count data (i.e., that the variance and the mean equal). This work instead introduces a flexible integer-valued moving average model for count data that contain over- or under-dispersion via the Conway-Maxwell-Poisson (CMP) distribution and related distributions. This first-order sum-of-Conway-Maxwell-Poissons moving average (SCMPMA(1)) model offers a generalizable construct that includes the PMA (among others) as a special case. We highlight the SCMPMA model properties and illustrate its flexibility via simulated data examples.
Cancer metabolism and intervention therapy
Authors: Zhao Huakan , Li Yongsheng 
Published: February 2021

AbstractMetabolic reprogramming with heterogeneity is a hallmark of cancer and is at the basis of malignant behaviors. It supports the proliferation and metastasis of tumor cells according to the low nutrition and hypoxic microenvironment. Tumor cells frantically grab energy sources (such as glucose, fatty acids, and glutamine) from different pathways to produce a variety of biomass to meet their material needs via enhanced synthetic pathways, including aerobic glycolysis, glutaminolysis, fatty acid synthesis (FAS), and pentose phosphate pathway (PPP). To survive from stress conditions (e.g., metastasis, irradiation, or chemotherapy), tumor cells have to reprogram their metabolism from biomass production towards the generation of abundant adenosine triphosphate (ATP) and antioxidants. In addition, cancer cells remodel the microenvironment through metabolites, promoting an immunosuppressive microenvironment. Herein, we discuss how the metabolism is reprogrammed in cancer cells and how the tumor microenvironment is educated via the metabolic products. We also highlight potential metabolic targets for cancer therapies.
Indigenous Slavery's Archive in Seventeenth-Century Chile
Authors: van Deusen Nancy E. 
Published: February 2021

AbstractThis article considers the creation and activation of certification documents codifying the capture-event and moment of enslavement of Reche-Mapuche people during the Araucanian wars with Spanish settlers in seventeenth-century Chile. Certification documents were normalized by the military bureaucracy and activated by slave owners who subjected and maintained Reche-Mapuche men, women, and children in bondage. These documents were foundational because they could reproduce what purportedly happened in other documentary and oral forms and facilitated the circulation of essentialized truths about the enslavement of individuals and about slavery writ large. In their legal petitions for freedom, Reche-Mapuche slaves had to speak against the grain of these legal instrumenta, which expressed a legally enforceable act or action as well as evidence of that action. Certification documents also had an archival afterlife following enactment of the abolition of Indigenous slavery in 1679.
A Party of Ex-convicts: Bolívar Ochart, Carceral Logics, and the Socialist Party in Early Twentieth-Century Puerto Rico
Authors: Meléndez-Badillo Jorell 
Published: February 2021

AbstractThis article uses the case of Bolívar Ochart, a midlevel member of the Socialist Party of Puerto Rico, to explore the ways that labor leaders navigated the Puerto Rican polity after the 1898 US occupation of the archipelago. The Socialist Party radically challenged the new carceral logics through its prison reform stance. Since it was the only political party in which most of its leadership had all been imprisoned, it also offered a space for formerly incarcerated, self-educated workers to become career politicians. Ultimately, this essay tells the story of how Ochart went from being a convict to receiving an executive pardon, publishing a groundbreaking book, and later becoming an elected official.
The Bandeirantes of Freedom: The Prestes Column and the Myth of Brazil's Interior
Authors: Blanc Jacob 
Published: February 2021

Abstract
The Prestes Column rebellion is among the most mythologized events in modern Brazil: from 1924 to 1927, a group of junior army officers marched nearly 15,000 miles through Brazil's vast interior regions. This Homeric epic into the so-called backlands launched the careers of some of Brazil's most important figures, and for nearly a century it has attained a mythic status in folklore and political history. Seeking to both explain and intervene in this legend, I argue that the myth of the Prestes Column emerged from and remained tethered to the stigmatized image of the interior. As a corrective to the column's dominant narrative and intervening in scholarship on myths more generally, this article reimagines the interior as both a place and an idea. The enduring symbolism of the backlands shows that exclusion, rather than a byproduct of national mythologies, is the pillar on which the ideas of inclusionary myths are based.
Infectious Dermatoses in Sport: A Review of Diagnosis, Management, and Return-to-Play Recommendations
Authors: Paradise Scott L. , Hu Yao-Wen Eliot 
Published: February 2021

Abstract
Infectious dermatoses represent a significant source of morbidity and missed athletic participation among athletes. Close quarters and skin trauma from contact sports can lead to outbreaks among teams and athletic staff. The National Collegiate Athletic Association and National Federation of State High School Associations have published guidance with recommended management and return-to-play criteria for common fungal, bacterial, viral, and parasitic rashes. In addition to rapidly diagnosing and treating infectious dermatoses, team physicians should counsel athletes and athletic staff on proper equipment care and personal hygiene to reduce infection transmission. Clinicians should always consult sport and athlete governing bodies for sport-specific recommendations.