bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of a carbonic anhydrase-Rubisco complex within the alpha-carboxysome
Authors: Blikstad Cecilia , Dugan Eli J. , Laughlin Thomas G. , Turnšek Julia B. , Liu Mira D. , Shoemaker Sophie R. , Vogiatzi Nikoleta , Remis Jonathan P. , Savage David F. 
Published: November 2021

ABSTRACTCarboxysomes are proteinaceous organelles that encapsulate key enzymes of CO2fixation - Rubisco and carbonic anhydrase - and are the centerpiece of the bacterial CO2concentrating mechanism (CCM). In the CCM, actively accumulated cytosolic bicarbonate diffuses into the carboxysome and is converted to CO2by carbonic anhydrase, producing a high CO2concentration near Rubisco and ensuring efficient carboxylation. Self-assembly of the α-carboxysome is orchestrated by the intrinsically disordered scaffolding protein, CsoS2, which interacts with both Rubisco and carboxysomal shell proteins, but it is unknown how the carbonic anhydrase, CsoSCA, is incorporated into the α-carboxysome. Here, we present the structural basis of carbonic anhydrase encapsulation into α-carboxysomes fromHalothiobacillus neapolitanus. We find that CsoSCA interacts directly with Rubisco via an intrinsically disordered N-terminal domain. A 1.98 Å single-particle cryo-electron microscopy structure of Rubisco in complex with this peptide reveals that CsoSCA binding is predominantly mediated by a network of hydrogen bonds. CsoSCA’s binding site overlaps with that of CsoS2 but the two proteins utilize substantially different motifs and modes of binding, revealing a plasticity of the Rubisco binding site. Our results advance the understanding of carboxysome biogenesis and highlight the importance of Rubisco, not only as an enzyme, but also as a central hub for mediating assembly through protein interactions.
Ubiquitin ligases and a processive proteasome facilitate protein clearance during the oocyte-to-embryo transition in Caenorhabditis elegans
Authors: Spike Caroline A. , Tsukamoto Tatsuya , Greenstein David 
Published: November 2021

ABSTRACTThe ubiquitin-mediated degradation of oocyte translational regulatory proteins is a conserved feature of the oocyte-to-embryo transition (OET). In the nematode Caenorhabditis elegans, multiple translational regulatory proteins, including the TRIM-NHL RNA-binding protein LIN-41/Trim71 and the Pumilio-family RNA-binding proteins PUF-3 and PUF-11, are degraded during the OET. Degradation of each protein requires activation of the M-phase cyclin-dependent kinase CDK-1, is largely complete by the end of the first meiotic division and does not require the anaphase promoting complex (APC). However, only LIN-41 degradation requires the F-box protein SEL-10/FBW7/Cdc4p, the substrate recognition subunit of an SCF-type E3 ubiquitin ligase. This finding suggests that PUF-3 and PUF-11, which localize to LIN-41-containing ribonucleoprotein particles (RNPs), are independently degraded through the action of other factors and that the oocyte RNPs are disassembled in a concerted fashion during the OET. We develop and test the hypothesis that PUF-3 and PUF-11 are targeted for degradation by the proteasome-associated HECT-type ubiquitin ligase ETC-1/UBE3C/Hu15, which is broadly expressed in C. elegans. We find that several GFP-tagged fusion proteins that are degraded during the OET, including fusions with PUF-3, PUF-11, LIN-41, IFY-1/Securin and CYB-1/Cyclin B, are incompletely degraded when ETC-1 function is compromised. However, it is the fused GFP moiety that appears to be the critical determinant of this proteolysis defect. These findings are consistent with a conserved role for ETC-1 in promoting proteasome processivity and suggest that proteasomal processivity is an important element of the OET during which many key oocyte regulatory proteins are rapidly targeted for degradation.Article SummaryThe ubiquitin-mediated degradation of translational regulatory RNA-binding proteins is a conserved feature of the oocyte-to-embryo transition (OET). C. elegans LIN-41 is a master regulator of oogenesis and is found in a large translational regulatory ribonucleoprotein (RNP) complex with more than 1000 maternal transcripts and the Pumilio-family RNA-binding proteins PUF-3 and PUF-11. We show that the concerted action of ubiquitin-mediated protein degradation and proteasome processivity rapidly disassemble LIN-41-containing RNPs during the OET thereby relieving repression of many maternal transcripts.
Pharmacologic rescue of circadian β-cell failure through P2Y1 purinergic receptor identified by small-molecule screen
Authors: Marcheva Biliana , Weidemann Benjamin J. , Taguchi Akihiko , Perelis Mark , Ramsey Kathryn Moynihan , Newman Marsha V. , Kobayashi Yumiko , Omura Chiaki , Fox Jocelyn E. Manning , Lin Haopeng , MacDonald Patrick E. , Bass Joseph 
Published: November 2021

SummaryThe mammalian circadian clock drives daily oscillations in physiology and behavior through an autoregulatory transcription feedback loop present in central and peripheral cells. Ablation of the core clock within the endocrine pancreas of adult animals impairs the transcription and splicing of genes involved in hormone exocytosis and causes hypoinsulinemic diabetes. However, identification of druggable proteins and pathways to ameliorate the burden of circadian metabolic disease remains a challenge. Here, we generated β cells expressing a nano-luciferase reporter within the proinsulin polypeptide to screen 2,640 pharmacologically-active compounds and identify insulinotropic molecules that bypass the secretory defect in clock mutant β cells. We validated lead compounds in primary mouse islets and identified known modulators of ligandgated ion channels and G-protein coupled receptors, including the antihelmintic ivermectin. Single-cell electrophysiology in circadian mutant mouse and human cadaveric islets validated ivermectin as a glucose-dependent secretagogue. Genetic, genomic, and pharmacologic analyses established that the molecular clock controls the expression of the purinergic P2Y1 receptor to mediate the insulinotropic activity of ivermectin. These findings identify the P2Y1 purinergic receptor as a target to rescue circadian β-cell failure and establish a chemical genetic screen for endocrine therapeutics.
Modelling the role of social media usage in improving users well-being: a social enhancement model perspective
Authors: Bodhi Rahul , Singh Tripti , Joshi Yatish 
Published: November 2021

PurposeEmployees have gradually adopted social media sites and their applications that have been associated with enhanced communication and collaboration at the workplace. However, social technologies have both positive as well as negative consequences. The current study examines the impact of loneliness on employees' psychological well-being (PW); subsequently, the mediating role of social media use intensity (SMI) at the workplace. It also examines the moderating role of gender and management status of employees.Design/methodology/approachThe present study conducted an online and offline survey using a cross-sectional design. Data were collected from 206 working professionals from the IT industry in India. Structural equation modelling was applied to analyse data.FindingsResults revealed that employee loneliness is positively associated with SMI. Employee's SMI was positively associated with enhanced PW. Unexpectedly, employee loneliness is positively and significantly related to PW. However, the moderating roles of gender and management status of employees were not supported.Practical implicationsThe current study can help managers, policymakers and organizations better understand the role of employee social media use in the workplace. Using the insights and understanding offered by the study, social media can be effectively utilized in the workplace. The study recommends that organizations may allow the use of social media at the workplace. Social media resources may also be helpful in improving employee communication and digital literacy.Originality/valueThe current study is a pioneer work and contributes to the literature by examining the relationship between loneliness, SMI and PW. This study has essential theoretical and managerial contributions.
Work–life balance, retention of professionals and psychological empowerment: an empirical validation
Authors: Panda Ansumalini , Sahoo Chandan Kumar 
Published: November 2021

PurposeThis study aims to explore the relationship between work–life balance and employee retention by examining the mediating role of psychological empowerment among software firms based in India.Design/methodology/approachThe study collected 283 responses by using a structured questionnaire and interview method. Structural equation modeling (SEM) was used to validate the hypothesized research model for examining the consistency and sturdiness of the study variables by applying AMOS 20.FindingsThe result reveals that psychological empowerment partially mediates the relationship between work–life balance and the retention of professionals. This indicates that a high degree of psychological empowerment strengthens the relations between work–life balance and the retention of professionals.Research limitations/implicationsThe research outlined a best-fit model of psychological empowerment as a partial mediator among work–life balance and the retention of professionals. The study presents a set of sensible and practical aspects where work–life balance and retention of professionals can aid in developing and generating commitment to the organization which could offer new insights for software professionals, managers and practitioners.Originality/valueThis study emphasized that psychological empowerment helps in enhancing dedication, loyalty, integrity, allegiance and trustworthiness among employees, thus playing a role between work–life balance and the retention of professionals.
Design and implementation of fake two-piece fully formed process for blouse
Authors: Zhan Biqin , Huang Xian , Cai Chenyuan , Cong Honglian 
Published: November 2021

PurposeFully formed knitting technology is a cutting-edge technology in the design and production of knitted apparel. Using this technology and its supporting design system, a new development mode of fully formed knitted apparel with double-layer structure and fake two-piece knitwear is proposed.Design/methodology/approachBased on the upper body structure feature points of human body characteristics and single-layer knitted garment prototype, a double-layer structure knitted garment pattern was established by pattern expansion method. The model was introduced into SDS-APPEX3 design system for process design, including three aspects consists: the inner vest, the outer blouse and double-layer joint part, analysis of the process and forming principle. Weaving on four-needle bed computerized flat knitting machine of MACH-2XS, through the setting of the machine parameters. Finally, a full-shaped fake two-piece knitted blouse was formed.FindingsOn the basis of single-layer knitted garment pattern, a double-layer garment pattern is constructed, and the design and weaving are completed on the four-needle bed computerized knitting machine of MACH-2XS and its supporting SDS-APPEX3 design system through the fake two-piece double-layer garment style design. The double-layer joint model is an effective reference for the construction of this kind of fake two-piece fully formed knitted clothing.Originality/valueIn this paper, a design and knitting method of fully formed double-layer structure fake two-piece knitted garment is proposed. The integrated knitting of fully formed double-layer structure sweater is realized for the first time, which provides ideas for the development of fully formed double-layer structure knitted clothing style and enriches the fully formed clothing style.
Earthen architectural heritage in the international context: values, threats, conservation principles and strategies
Authors: Mileto Camilla , Vegas López-Manzanares Fernando 
Published: November 2021

PurposeThis research aims to highlight the values, principles and recommendations for conservation in order to establish valid strategies for the conservation of earthen built heritage. This is done following a methodology which uses indirect (bibliography) and direct (case study) sources systematically analysed from different perspectives: the values of Earth as a material and of architectural and vernacular heritage; the heritage conservation principles found in international documents; and the analysis of over 3,000 case studies from which good practices in earthen architecture conservation are extracted.Design/methodology/approachEarthen built architectural heritage is found widely in all parts of the world, in archaeological sites and monumental and vernacular architecture, which research centres and researchers are increasingly studying and cataloguing. However, despite its richness and historic and cultural values, as well as its many merits in environmental sustainability, sociocultural and socio-economic terms, the value of this heritage has not been fully recognized in fields with major repercussions in conservation.FindingsFinally, these data are cross-referenced to establish the broadest possible strategies to guarantee all aspects to be taken into account in the conservation of earthen built architectural heritage.Originality/valueThe text provides an overview of the different methodologies in order to extract specific strategies applicable to the conservation of this heritage, both locally and globally.
Contactless service encounters among Millennials and Generation Z: the effects of Millennials and Gen Z characteristics on technology self-efficacy and preference for contactless service
Authors: Kim Songmee , Jang Seyoon , Choi Woojin , Youn Chorong , Lee Yuri 
Published: November 2021

Purpose“Contactless service” refers to the use of technology in providing products or services without a salesperson. This study explores the mechanism underlying Millennial and Generation Z (M/Z generations) consumers' preference for contactless service over salespersons in retail stores. In addition, this study tests differences between the M/Z generations.Design/methodology/approachThe researchers predict characteristics to be antecedents of young consumer's preference for contactless service over salespersons and that the effects are mediated by technology self-efficacy. Next, a moderating variable (perceived consumer conformity) is added in the path between technology self-efficacy and the preference for contactless service. The hypotheses are tested among 142 Gen Z and 137 Millennial respondents.FindingsThe results show that M/Z generations’ characteristics significantly influence the preference for contactless service, except for security seeking. Also, interests in new technology and safety seeking are perceived higher by M/Z generations. The influence of technology self-efficacy on the preference for contactless service is moderated by social conformity.Originality/valueAs retail technology rapidly develops, the service industry is expected to change from the past, where salespersons played an important role, to contactless services. This study has academic and practical values, for the authors clarify the underlying psychological mechanisms of why young consumers prefer retail technology rather than communication with salespersons.
To pay or not to pay: understanding mobile game app users' unwillingness to pay for in-app purchases
Authors: Salehudin Imam , Alpert Frank 
Published: November 2021

PurposeWorldwide In-app Purchase (IAP) revenues reached almost US$37 billion in 2017 and doubled that in 2020. Although the revenue from IAPs exceeds those from paid apps, only 5% of total app users make any IAPs. This paper investigates why some users will not make IAPs and develop a novel concept of users' Perceived Aggressive Monetization of IAPs as an alternative framework to explain IAP behavior.Design/methodology/approachGiven the newness of IAPs, this study uses qualitative research to understand the phenomenon and develop a model to explain the decision to spend on IAPs. In total, this study collected 4,092 unique user-generated comments from app user review sites and social media webpages where users discuss in-app purchasing.FindingsThe analysis reveals recurring themes that explain user unwillingness to make in-app purchases, such as conflicting meanings of free-to-play, perceived unfairness and aggressive monetization of IAP by app publishers, and self-control issues. Subsequent user interviews support the themes and suggest that IAP spending might be more impulsive.Originality/valueThe paper develops a new concept of perceived aggressive monetization. Additionally, it proposes a novel theoretical framework that future researchers can use to understand why some mobile game users are unwilling to pay for IAPs.
Local food research: a bibliometric review using Citespace II (1970–2020)
Authors: Şahin Ayşe , Yılmaz Gökhan 
Published: November 2021

PurposeIn recent years, one of the most important and active research areas has been local food. This study aims to examine the evolution and trends of local food research in several disciplines or fields in the 50 years, highlighting emerging research themes, collaborations such as authors, institutions, journals and co-citations such as documents, authors and journals.Design/methodology/approachA total of 869 local food-related articles from the Web of Science (WoS) database from 1970 to 2020 were analyzed by several bibliometric analyses using CiteSpace II software.FindingsThe findings indicate that the number of studies in the local food field has increased rapidly and it is an interdisciplinary subject. Moreover, the results indicate that there is partly collaboration among authors, institutions or countries that conduct research on local food. For example, the most productive authors in this field are in USA, India, Brazil and European countries. Another remarkable result of this study is that tourism and hospitality discipline has focused on local food subject, especially in the last five years.Originality/valueThis is one of the first attempts by using network analysis to directly understand and visualize the interrelated structures of the local food research. Furthermore, the results of this study may provide researchers a reference guide to better evaluate and monitor local food research.
Infoecology of the deep learning and smart manufacturing: thematic and concept interactions
Authors: Asemi Asefeh , Ko Andrea , Asemi Adeleh 
Published: November 2021

PurposeThis infecological study mainly aimed to know the thematic and conceptual relationship in published papers in deep learning (DL) and smart manufacturing (SM).Design/methodology/approachThe research methodology has specific research objectives based on the type and method of research, data analysis tools. In general, description methods are applied by Web of Science (WoS) analysis tools and Voyant tools as a web-based reading and analysis environment for digital texts. The Yewno tool is applied to draw a knowledge map to show the concept's interaction between DL and SM.FindingsThe knowledge map of DL and SM concepts shows that there are currently few concepts interacting with each other, while the rapid growth of technology and the needs of today's society have revealed the need to use more and more DL in SM. The results of this study can provide a coherent and well-mapped road map to the main policymakers of the field of research in DL and SM, through the study of coexistence and interactions of the thematic categories with other thematic areas. In this way, they can design more effective guidelines and strategies to solve the problems of researchers in conducting their studies and direct. The analysis results demonstrated that the information ecosystem of DL and SM studies dynamically developed over time. The continuous conduction flow of scientific studies in this field brought continuous changes into the infoecology of subjects and concepts in this area.Originality/valueThe paper investigated the thematic interaction of the scientific productions in DL and SM and discussed possible implications. We used of the variety tools and techniques to draw our own perspective. Also, we drew arguments from other research work to back up our findings.
Betas in the time of corona: a conditional CAPM approach using multivariate GARCH model for India
Authors: Jain Sonali 
Published: November 2021

PurposeThis paper empirically investigates the effect of the coronavirus pandemic (COVID-19) on the Indian financial market and firm betas, perhaps the first paper to do so. The results will be helpful for investors tracking betas during future the coronavirus waves.Design/methodology/approachA conditional capital asset pricing model (CAPM) and multivariate generalized autoregressive conditional heteroskedasticity (GARCH) model is used to estimate time-varying daily betas of the 50 largest Indian stocks spread across 16 industries over five years (Nov 2017 to May 2021), including the two waves of COVID-19 in India.FindingsThe results show that the betas increased during the COVID wave-1 (2020) but not during COVID wave-2 (2021). Moreover, the increase is more pronounced for consumer goods, infrastructure, insurance and information technology, unlike energy (oil and gas, power and mining) industries. Further, there are positive abnormal residual returns during the COVID waves. The results will be helpful for investors tracking betas during future COVID-19 waves.Originality/valueThis is perhaps the first paper to study the firm betas in light of the COVID-19 pandemic.
Social media: the double-edged sword for perpetuating and preventing criminal activities in Zimbabwe
Authors: Mugari Ishmael , Muzinda Caleb 
Published: November 2021

PurposeThis study aims to evaluate social media’s role in facilitating criminal activities in Zimbabwe and determine how social media platforms have been embraced by the Zimbabwean police to curb criminal activities.Design/methodology/approachA total of 72 participants from the Zimbabwean police and 5 non-police security experts were invited to participate in this study through questionnaires and in-depth interviews. The study was confined to an urban policing area, which is policed by approximately 400 police officers.FindingsThe facilitation of violent protests was found to be the major threat that is posed by social media in Zimbabwe, with social media platforms playing a catalytic role in fuelling these violent protests. Social media is also playing a significant role in the spread of fake news, with the intention of causing fear and panic amongst citizens. Findings also reveal that the Zimbabwe police have embraced social media platforms for sharing crime prevention tips, receiving crime reports, engaging with citizens and gathering crime intelligence.Originality/valueThe study provides context-specific information on the threats posed by social media on a developing nation that has been characterised by a myriad of socio-economic challenges, as well as how these social media platforms have been embraced for crime prevention and control. Given limited empirical research on social media and national security in Zimbabwe, this study adds to the existing body of knowledge.