bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a Prognostic Signature of Autophagy-Related lncRNAs in Non-Small Cell Lung Cancer
Authors: Zhang Xinyang , Cao Yu , Chen Li 
Published: March 2021

Abstract
Background: Autophagy inhibits tumorigenesis by limiting inflammation, LncRNA regulates gene expression levels in the form of RNA at various levels, so both of them are closely related to the occurrence and development of tumors.Methods: 232 autophagy-related genes were used to construct a co-expression network to extract autophagy-related lncRNAs. A prognostic signature was constructed by multivariate regression analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) was applied to analyze pathway enrichment in cancer pathways. Immunoinfiltration analysis was used to analyze the relationship between the prognostic model and the tumor.Results: Nine autophagy-related lncRNAs were used to construct a prognostic model for non-small cell lung cancer. The median value of the value at risk was used to distinguish between the high and low risk groups, and the low-risk group had better survival. Because the KEGG pathway analysis showed that the prognostic model was enriched in some immune pathways, further exploration of immune infiltration was conducted and it was found that the prognostic model did play a unique role in the immune microenvironment. And the prognostic model was associated with clinical factors.Conclusion: The prognostic model of autophagy-related lncRNAs constructed by us can predict the prognosis of non-small cell lung cancer.
Association between Serum Anti-glycopeptidolipid-core IgA Antibody Titers and Clinical Characteristics of Mycobacterium Avium Complex Pulmonary Disease
Authors: Kodaka Norio , Nakano Chihiro , Oshio Takeshi , Watanabe Kayo , Niitsuma Kumiko , Imaizumi Chisato , Shimada Nagashige , Morita Hirotsugu , Matsuse Hiroto 
Published: March 2021

Abstract
Mycobacterium avium complex pulmonary disease (MAC-PD) can be serologically diagnosed according to the presence of anti-glycopeptidolipid (GPL)-core IgA antibodies. However, few studies have examined the association between serum anti-GPL-core IgA antibody titers and the clinical characteristics of patients with MAC-PD. From April 2014 to June 2019, we determined the level of anti-GPL-core IgA antibodies in 489 MAC-PD patients at our institute. Of them, 89 patients fulfilled the criteria of the American Thoracic Society and the Infectious Diseases Society of America statement on the diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. These patients were divided into antibody-positive (n = 59) or -negative (n = 30) groups according to their serum anti-GPL-core IgA antibody results. Additionally, the positive antibody group was further divided into a strong positive group (n = 27) and a weak positive group (n = 32), and their clinical characteristics were retrospectively compared. Disease progression requiring treatment during the 12 months following diagnosis and extensive radiological findings were significantly abundant in the strong positive group compared with the weak positive group. Our findings revealed that serum anti-GPL-core IgA antibody titers are useful not only for diagnosing MAC-PD but also for predicting the risk of exacerbation.
Analysis and Farmers Perception of Climate Change Trends in Kashmir Region of Union Territory-Jammu &amp; Kashmir, India: North Western Himalayas
Authors: Mahdi S. Sheraz , Dhekale Bhagyashree Shankarao , Hussain Ashaq , Jehangir Intikhab Aalum , Jan Rukhsana , Bhat M. Anwar , Sofi Najeebul Rehman , Ahmed Latief , Qureshi Asif M. Iqbal , Aezum Aziz Mujtaba , Bangroo Shabir A. , Wani Owais Ali , Bahar F.A. 
Published: March 2021

Abstract
Analysis of climatic variables is important for detection and attribution of climate change trends and has received a considerable attention from researchers across the globe including India. Kashmir valley of newly formed Union Territory Jammu & Kashmir situated in north western part of India is having a rich repository of glaciers, a small change in the precipitation and temperature management could introduce about environmental, agricultural and economic penalties. To this end, current study aims to analyse changing patterns in precipitation and temperature variables over the various elevation zones of the Kashmir Valley using long term precipitation and temperature data obtained from National Data Centre, Indian Meteorological Department (IMD), Pune for the period of 40 years (1980–2019). The results revealed that average mean minimum and maximum temperature of the Kashmir valley has increased substantially at a rate of 0.02oC/year. Warming trends has been observed in all seasons, however, winter and spring season temperatures have shown statistically significant increasing trends. In addition, mean maximum and minimum temperature in plain and mountain areas have reported higher rates of increase in comparison to Karewah’s and foothill areas of Kashmir. Study of annual precipitation results for the same period indicates a diminishing pattern with a rate of -5.01 mm/year. Seasonal precipitation was also found decreasing at rate of -4.95, -0.30, -0.28 and − 0.06 mm/year for the spring, winter, autumn and summer seasons respectively and at different elevation zones, higher rates of precipitation decline have been observed in the mountainous area, which can be very detrimental to the agricultural crops of the Kashmir valley through water supply, climate regulation and ground water recharge. Further, the above statistical test results of increase in temperature and decrease in precipitation over different topographical zones of Kashmir were corroborated with the information attained from interview and involvement of the small farmer holders of 06 different locations representing the whole Kashmir and has been discussed in this paper to get a clearer understanding of climate change related instability and patterns in weather variables in the Kashmir Valley.
Active Components from Lagotis Brachystachya Maintain Uric Acid Homeostasis by Inhibiting Renal TLR4-NLRP3 Signaling in Hyperuricemic Mice
Authors: Zhu Ji-Xiao , Yang Hai-Yan , Hu Wei-Qiong , Cheng Jie , Liu Yang , Yi Li-Tao , Cheng Hong-Yu 
Published: March 2021

Abstract
Lagotis brachystachya Maxim is an herb widely used in traditional Tibet medicine. Our previous study indicated that total extracts from Lagotis brachystachya could lower uric acid levels. This study aimed to further elucidate the active components (luteolin, luteoloside and apigenin) isolated from Lagotis brachystachya and the underlying mechanism in vitro and vivo. The results showed that treatment with luteolin and luteoloside reversed the reduction of organic anion transporter 1 (OAT1) levels, while apigenin attenuated the elevation of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) levels in uric acid-treated HK-2 cells, which were consistent with the finding in the kidney of potassium oxonate (PO)-induced mice. On the other hand, hepatic xanthine oxidase activity was inhibited by the components. In addition, all of these active components improved the morphology of the kidney in hyperuricemic mice. Moreover, molecular docking showed that luteolin, luteoloside and apigenin could bind TLR4 and NLRP3. Consistently, western blot showed that the components inhibited TLR4/MyD88/NLRP3 signaling. In conclusion, these results indicated that luteolin, luteoloside and apigenin could attenuate hyperuricemia by decreasing the production and increasing the excretion of uric acid, which were mediated by the inhibition of inflammatory signaling pathways.
Effects of different vasopressors on the contraction of the superior mesenteric artery and uterine artery in rats during late pregnancy
Authors: Wang Tingting , Liao Limei , Tang Xiaohui , Li Bin , Huang Shaoqiang 
Published: March 2021

Abstract
Background：Hypotension after neuraxial anaesthesia is one of the most common complications during caesarean section. Vasopressors are generally agreed to be the most effective way to improve hypotension, but it is unclear which of these drugs is best for caesarean section. We assessed the effects of vasopressors on the contractile response of uterine arteries and superior mesenteric arteries in pregnant rats, with the goal of identifying a drug that raises the blood pressure of the systemic circulation while minimally affecting the uterine and placental circulation. Methods： Isolated ring segments from the uterine and superior mesenteric arteries of pregnant rats were mounted in organ baths, and their contractile responses to several vasopressor agents were studied. Concentration-response curves for norepinephrine, phenylephrine, metaraminol and vasopressin were constructed. Results：The experimental results showed that the contractile response of the mesenteric artery to norepinephrine, as measured by the pEC50 of the drug, was stronger than that of the uterine artery (5.617 ± 0.11 vs. 4.493 ± 1.35, p=0.009), and the contractile response of the uterine artery to metaraminol was stronger than that of the mesenteric artery (pEC50: 5.084±0.17 vs 4.92±0.10, p=0.007). There was no statistically significant difference in the pEC50 of phenylephrine or vasopressin between the two blood vessels. Conclusion： In vitro experiments show that compared with phenylephrine, metaraminol , vasopressin, norepinephrine can contract peripheral blood vessel more strongly, while having the least effect on the contraction of uterine artery. These findings provide some support for the use of norepinephrine in mothers between the time of neuraxial anaesthesia and the delivery of the foetus.
WITHDRAWN: Apigenin neutralizes the inhibitory effect of inflammation on the osteogenic differentiation of human mesenchymal stem cells
Authors: Asadi Azita , Goudarzi Farjam , Ghanadian Mustafa , Mohammadalipour Adel 
Published: March 2021

Abstract
Background: The stimulating effects of apigenin on mesenchymal stem cells (MSCs) osteogenesis, as well as the anti-inflammatory effect of this flavonoid, have been identified. In this study, osteogenic differentiation was investigated under inflammatory conditions and treatment with apigenin. Methods and Results: Along with osteogenic differentiation of MSCs, they became inflamed with LPS/PA, and treated simultaneously with apigenin. The degree of differentiation was assessed by alizarin red staining and alkaline phosphatase (ALP) activity. Also, gene expression of NLRP3 and RUNX2 was performed along with protein expression of IL-1β. Significant increase in NLRP3 and IL-1β were observed in MSCs when exposed to LPS/PA (p<0.01). Also, the osteogenesis was significantly decreased (p<0.01). Apigenin treatment induced significantly higher gene expression of RUNX2, the activity of ALP, and cell staining (p<0.01) which were also associated with reduced inflammation in these cells. Conclusions: The effectiveness of apigenin on osteogenesis under inflammatory conditions was cautiously observed.
Orthorexia Nervosa and Substance Use for the Purposes of Weight Control, Conformity, and Emotional Coping
Authors: Oberle Crystal D. , Marcell Haley S. , Noebel Natalie A. 
Published: March 2021

Abstract
Purpose: Orthorexia nervosa (ON), characterized by extreme behaviors driven by the goal of eating only healthy and pure foods, could plausibly be associated with avoidance of nicotine, alcohol, and illicit drugs. However, findings from the limited research on these relationships are mixed, and other eating disorders are associated with greater substance abuse. Method: An online survey was completed by 471 participants (86% women, mean age = 20) recruited from undergraduate courses and through an Instagram advertisement. The questionnaires assessed ON symptomatology; frequency of smoking, alcohol consumption, and illicit drug use; abuse of these substances; and motivations for using these substances. Results: ON scores were not significantly related to the level of use or abuse of nicotine, alcohol, or most illicit drugs. Yet, ON scores were positively correlated with frequency of using illicit depressant drugs. Further, among substance users, ON scores were positively associated with smoking or vaping for the purpose of weight control, and with consuming alcohol and using illicit drugs for the purposes of conformity and coping with such negative emotions as anxiety and depression. Conclusion: Although people who are high in ON symptomatology may be at least partly driven by a strong desire to be as healthy as possible, they are not less likely to use potentially harmful drugs. Instead, many of them may even turn to certain drugs for the same weight-control and emotional-coping motives that guide the behaviors of individuals with other eating disorders.Level of evidence: Level V, descriptive cross-sectional study
Biogeochemical Causes of Sixty-Year Trends and Seasonal Variations of River Water Properties in a Large European Basin
Authors: Kopáček Jiří , Hejzlar Josef , Porcal Petr , Znachor Petr 
Published: March 2021

Abstract
We evaluated long-term trends and seasonal variations in the major physical-chemical properties of the circum-neutral Slapy reservoir (Vltava, Czech Republic) from 1960 to 2019. Mean annual water temperature increased by 2.1 °C, flow maxima shifted by ~13 days from the early April to mid-March, and the onset of thermal stratification of water column and spring algal peaks advanced by 19 and 21 days, respectively, due to climate warming. Concentrations of major ions, phosphorus (P), and chlorophyll increased from the 1960s to the 1990s–2000s, then decreased due to changing agricultural practices and legislation, intensified wastewater treatment, and decreasing atmospheric pollution. Concentrations of dissolved organic carbon (DOC) decreased from 1960 to the 1990s due to improved wastewater treatment, then began to increase in response to climate change and reduced acidic deposition. Concentrations of water constituents exhibited varying individual long-term and seasonal patterns due to the differing effects of following major processes on their production/removal in the catchment-river system: (1) applications of synthetic fertilizers, liming and farmland draining (NO3–, SO42–, Cl–, Ca2+, Mg2+, K+, and HCO3–), (2) wastewater production and treatment (DOC, P, N forms), (3) road de-icing (Cl– and Na+), (4) atmospheric pollution (SO42–), (5) climate change (DOC), and (6) the aging of reservoirs (NH4+). The water pH increased until the early 1990s, then decreased and exhibited pronounced seasonal variations, integrating the effects of changing external acidity sources and in-lake H+ sources and sinks (i.e., microbial CO2 production/consumption and availability and transformations of inorganic N), and changes in water buffering capacity. Anthropogenic and climatic effects, reservoir aging, and changes in water eutrophication thus may significantly affect water pH also in circum-neutral systems.
Research on the Effects of Yttrium on Bismuth Titanate Borosilicate Glass System&nbsp;
Authors: shaaban Kh. S. 
Published: March 2021

Abstract
Glasses with the chemical composition of 52B2O3 – 12SiO2- 26Bi2O3 – (10 - x)TiO2 - xY2O3, (0 ≤ x ≥ 10)prepared using the melt-quench method. The goal of this study is to investigate the structural, mechanical, and radiation shielding characteristics of these samples. XRD analysis has explored the nature of the glass system. Molar volume obtained reduced while the density denotes increased in the present system. As the molar volume decrease inter-ionic distance, polaron radius, inter-nuclear distance, and Y-Y separation of the investigated glasses decreased. The mechanical characteristics depend on the glass structure of the current glasses sample. Ultrasonic velocities and elastic moduli (experimental and theoretical) for these glasses obtained observed to get enhanced. The radiation shielding efficiency investigated by Phy-X/PSD software. The mass attenuation coefficient (μ/ρ) mean free path (MFP), half-value layer (HVL), tenth value layer (TVL), and effective atomic number (Zeff), of glasses, have designed to simulate for gamma photon energies between 0.015 and 15 MeV.
Low-Frequency Earthquakes along the Ryukyu Islands Triggered by Teleseismic Earthquakes
Authors: Kinjo Ayumi , Nakamura Mamoru 
Published: March 2021

Abstract
Tremors and low-frequency earthquakes (LFEs), which occur in the plate interface, can provide useful information about the state of aseismic stress transfer in mega-earthquake fault zones. We estimated the distribution of stress sensitivity in the subducted plate interface by using triggered LFEs. Specifically, we detected LFEs in the Ryukyu Trench triggered by the surface waves of large teleseismic earthquakes by using the waveform records of broadband and short-period seismometers installed in the Ryukyu Arc. We selected a total of 45 teleseismic earthquakes with magnitudes of more than 7.5, which occurred between 2004 and 2017, for the analysis. We could detect the triggered LFEs for five teleseismic earthquakes. Then, we determined the hypocenters of LFEs by using the relative arrival times of LFEs for each station. The LFEs were distributed in the south of Okinawa Island and the Yaeyama area. Moreover, they were distributed around the source fault of the slow slip events. These were almost the same as the position of LFEs accompanying very low-frequency earthquakes (VLFEs). However, the epicenters of the triggered LFEs were concentrated near the locations of the most active LFE clusters accompanying VLFEs. This suggests that the sensitivity for inducing LFEs was higher near the most active clusters of the LFEs accompanying the VLFEs. The amplitudes of the triggered LFEs were proportional to the peak ground velocity of the surface waves. This indicates that the LFEs accompanying VLFEs are activated by stress acceleration in the Yaeyama and Okinawa areas and the triggered LFEs observed in these areas can be a result of the activation of the ambient tremors due to increased stress.
Application of C-reactive Protein/Albumin Ratio (CAR) in Kawasaki Disease
Authors: Wang Zhenquan , Shao Yiping , Rong Xing , Qiu Huixian , Wang Jinxing , Zhang Xuting , Hang Biyao , Xu Zhipeng , Wu Rongozhou , Chu Maoping 
Published: March 2021

Abstract
Objective: To investigate the association between the C-reactive protein/albumin ratio (CAR) and coronary artery lesions (CAL), intravenous immunoglobulin (IVIG) resistance in children with Kawasaki disease (KD).Methods: We retrospectively studied 753 children with KD, categorizing them into the CAL group(n=238) and the No-CAL group(n=515), the IVIG-resistance group(n=61) and the No-IVIG- resistance(n=653) group. The differences in laboratory data, clinical manifestations, the relationship between CAR and CAL as well as IVIG resistance were compared between the two cohort groups.Results: Compared with No-CAL group, KD with CAL had a higher CAR (2.12 vs 1.69, p <0.001). And CAR was significantly higher in KD children with IVIG resistance (2.42 vs 1.85, p<0.001). Multivariable logistic regression analysis demonstrated that higher CAR was a risk factors of CAL(OR=1.198, p<0.001) and IVIG resistance (OR=1.297, p<0.001), respectively. CAL and IVIG resistance interact with each other. ROC curve analysis performed for the prediction of CAL, the best cut-off point for CAR was 1.80(AUC=0.602, sensitivity 64.7%, specificity 54.8%). When predicting IVIG resistance according to the ROC curve analysis, the optimal cutoff point for CAR was 2.20(AUC=0.621, sensitivity 59.0%, specificity 61.1%).Conclusions: CAR is a valid indicator in KD children. Higher CAR may be helpful in predicting CAL and IVIG resistance in KD.
Filtering high-dimensional methylation marks with extremely small sample size: an application to gastric cancer data
Authors: Chen Xin , Zhang Qingrun , Chekouo Thierry 
Published: March 2021

Abstract
Background: DNA methylations in critical regions are highly involved in cancer pathogenesis and drug response. However, to identify causal methylations out of a large number of potential polymorphic DNA methylation sites is challenging. This high-dimensional data brings two obstacles: first, many established statistical models are not scalable to so many features; second, multiple-test and overfitting become serious. To this end, a method to quickly filter candidate sites to narrow down targets for downstream analyses is urgently needed. Methods: BACkPAy is a pre-screening Bayesian approach to detect biological meaningful clusters of potential differential methylation levels with small sample size. BACkPAy prioritizes potentially important biomarkers by the Bayesian false discovery rate (FDR) approach. It filters non-informative sites (i.e. non-differential) with flat methylation pattern levels accross experimental conditions. In this work, we applied BACkPAy to a genome-wide methylation dataset with 3 tissue types and each type contains 3 gastric cancer samples. We also applied LIMMA (Linear Models for Microarray and RNA-Seq Data) to compare its results with what we achieved by BACkPAy. Then, Cox proportional hazards regression models were utilized to visualize prognostics significant markers with The Cancer Genome Atlas (TCGA) data for survival analysis. Results: Using BACkPAy, we identified 8 biological meaningful clusters/groups of differential probes from the DNA methylation dataset. Using TCGA data, we also identified five prognostic genes (i.e. predictive to the progression of gastric cancer) that contain some differential methylation probes, whereas no significant results was identified using the Benjamin-Hochberg FDR in LIMMA. Conclusions: We showed the importance of using BACkPAy for the analysis of DNA methylation data with extremely small sample size in gastric cancer. We revealed that RDH13, CLDN11, TMTC1, UCHL1 and FOXP2 can serve as predictive biomarkers for gastric cancer treatment and the promoter methylation level of these five genes in serum could have prognostic and diagnostic functions in gastric cancer patients.
ST-Segment Resolution as a Marker for Myocardial Scar in ST-Segment Elevation Myocardial Infarction
Authors: Dong Qian , Wen Xuesong , Chang Guanglei , Xia Rui , Wang Sihang , Yang Yunjing , Tao Yi , Zhang Dongying , Shu Qin 
Published: March 2021

Abstract
Objective: To investigate the relationship between ST-segment resolution (STR) and myocardial scar thickness after percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI).Methods: Forty-two STEMI patients with single-branch coronary artery stenosis or occlusion were enrolled. ST-segment elevations were measured at emergency admission and at 24 h after PCI. Late gadolinium-enhanced cardiac magnetic resonance imaging (CMR-LGE) was performed 7 days after PCI to evaluate myocardial scars. Statistical analyses were performed to assess the utility of STR to predict the development of transmural (>75%) or non-transmural (<75%) myocardial scars.Results: The sensitivity and specificity of STR for predicting transmural scars were 96% and 88%, respectively, at an STR cut-off value of 40.15%. The area under the curve was 0.92. Multivariate logistic proportional hazards regression analysis disclosed that patients with STR<40.15% had a 112.95-fold higher probability of developing transmural scars compared with patients with STR≥40.15%. STR percentage was negatively correlated with myocardial scar thickness (β=-0.838, P<0.001) and size (β=-0.714, P<0.001).Conclusion: STR<40.15% at 24 h after PCI may provide meaningful diagnostic nformation regarding the extent of myocardial scarification in STEMI patients.