bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular epidemiology and genotype/subtype distribution of Blastocystis sp., Enterocytozoon bieneusi, and Encephalitozoon spp. in livestock: concern for emerging zoonotic infections
Authors: Mohammad Rahimi Hanieh , Mirjalali Hamed , Zali Mohammad Reza 
Published: September 2021

AbstractIntestinal parasitic infections have high prevalence rate in many regions especially in developing countries. The aim of this study was to determine the presence and genotype/subtype of some intestinal protozoa in livestock in Iran. Stool samples were collected from cattle, sheep, chickens, and horses. The presence of targeted parasites was evaluated using real-time PCR. Genotyping/subtyping of positive samples was characterized using sequencing of the ITS and barcoding region, respectively. Blastocystis sp., 27.7% (48/173) and Enterocytozoon bieneusi 26.0% (45/173) were the most frequent protozoa followed by Encephalitozoon spp., 0.57% (1/173). Cryptosporidium spp. were not detected among samples. Encephalitozoon spp., was detected only in chickens 2.2% (1/45). A statistically correlation was seen between animals and the prevalence of targeted protozoa. E. bieneusi genotypes I (9/38; 23.68%), BEB6 (22/38; 57.89%), D (6/38; 15.79%), and horse1 (1/38; 2.63%) were detected among samples. A statistically significant correlation was seen between the genotypes and animals (P ≤ 0.05). Blastocystis sp., ST1 (1/45; 2.22%), ST5 3/45; 6.66%), ST7 (1/45; 2.22%), ST10 (24/45; 53.33%), and ST14 (16/45; 35.55%) were characterized among samples. There was no significant correlation between certain subtypes and animals (P = 0.173). The presence of zoonotic potential genotypes of E. bieneusi in animals and zoonotic potential subtypes ST1 and ST7 among our samples provide a clue about the transmission dynamic of E. bieneusi and Blastocystis sp. between animals–animals and humans–animals.
Revealing the molecular interplay of curcumin as Culex pipiens Acetylcholine esterase 1 (AChE1) inhibitor
Authors: Rao Priyashi , Goswami Dweipayan , Rawal Rakesh M. 
Published: September 2021

AbstractEmergence of vector borne diseases has continued to take toll on millions of lives since its inception. The use of insecticides began as vector control strategy in the early 1900’s but the menace of insects is still prevalent. Additionally, the inadequate use of organophosphates and carbamates which target acetylcholine esterase (AChE), are known to develop resistance amongst vectors of transmission and are toxic to humans. In this study, extensive computational screening was performed using homology modelling, molecular docking, molecular dynamics (MD) simulation and free energy change calculation, which highlighted curcumin as a lead molecule out of ~ 1700 phytochemicals against Culex pipiens AChE. In vivo larvicidal activity was carried out along with in vivo and in vitro AChE inhibition assay to determine the biochemical efficacy of curcumin. Our study reveals that curcumin induces mortality in Cx. pipiens at an early stage of its life cycle by AChE inhibition. This also underlines the use of curcumin as a coming-age natural product insecticide.
Control of axillary bud growth in tobacco through toxin gene expression system
Authors: Lv Jing , Chen Ya-Qiong , Ding An-Ming , Lei Bo , Yu Jing , Gao Xiao-Ming , Dai Chang-Bo , Sun Yu-He 
Published: September 2021

AbstractThe control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study, a toxin gene control system for plants based on dexamethasone (DEX) induction was constructed, and the positive transgenic tobacco exhibited growth retardation in the application area (axillary bud). The expression level of the lethal Diphtheria toxin A (DTA) gene under different DEX concentrations at different application days was analyzed. The highest expression levels appeared at 5 days after the leaf injection of DEX. The DTA transcripts were induced by 5 µM DEX and peaked in response to 50 µM DEX at 5 days after leaf injection. Here, a chemical induction system, combined with a toxin gene, were used to successfully control the growth of tobacco axillary buds after topping. The DTA expression system under DEX induction was sensitive and efficient, therefore, can be used to control axillary bud growth and development in tobacco.
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells
Authors: Park Hae-Ryung , Vallarino Jose , O’Sullivan Michael , Wirth Charlotte , Panganiban Ronald A. , Webb Gabrielle , Shumyatcher Maya , Himes Blanca E. , Park Jin-Ah , Christiani David C. , Allen Joseph , Lu Quan 
Published: September 2021

AbstractThe widespread use of electronic cigarettes (e-cig) is a serious public health concern; however, mechanisms by which e-cig impair the function of airway epithelial cells—the direct target of e-cig smoke—are not fully understood. Here we report transcriptomic changes, including decreased expression of many ribosomal genes, in airway epithelial cells in response to e-cig exposure. Using RNA-seq we identify over 200 differentially expressed genes in air–liquid interface cultured primary normal human bronchial epithelial (NHBE) exposed to e-cig smoke solution from commercial e-cig cartridges. In particular, exposure to e-cig smoke solution inhibits biological pathways involving ribosomes and protein biogenesis in NHBE cells. Consistent with this effect, expression of corresponding ribosomal proteins and subsequent protein biogenesis are reduced in the cells exposed to e-cig. Gas chromatography/mass spectrometry (GC/MS) analysis identified the presence of five flavoring chemicals designated as ‘high priority’ in regard to respiratory health, and methylglyoxal in e-cig smoke solution. Together, our findings reveal the potential detrimental effect of e-cig smoke on ribosomes and the associated protein biogenesis in airway epithelium. Our study calls for further investigation into how these changes in the airway epithelium contribute to the current epidemic of lung injuries in e-cig users.
Magnetic silica particles functionalized with guanidine derivatives for microwave-assisted transesterification of waste oil
Authors: Chipurici Petre , Vlaicu Alexandru , Călinescu Ioan , Vînătoru Mircea , Busuioc Cristina , Dinescu Adrian , Ghebaur Adi , Rusen Edina , Voicu Georgeta , Ignat Maria , Diacon Aurel 
Published: September 2021

AbstractThis study aimed to develop a facile synthesis procedure for heterogeneous catalysts based on organic guanidine derivatives superbases chemically grafted on silica-coated Fe3O4 magnetic nanoparticles. Thus, the three organosilanes that were obtained by reacting the selected carbodiimides (N,N′-dicyclohexylcarbodiimide (DCC), N,N′-diisopropylcarbodiimide (DIC), respectively 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) with 3-aminopropyltriethoxysilane (APTES) were used in a one-pot synthesis stage for the generation of a catalytic active protective shell through the simultaneous hydrolysis/condensation reaction with tetraethyl orthosilicate (TEOS). The catalysts were characterized by FTIR, TGA, SEM, BET and XRD analysis confirming the successful covalent attachment of the organic derivatives in the silica shell. The second aim was to highlight the capacity of microwaves (MW) to intensify the transesterification process and to evaluate the activity, stability, and reusability characteristics of the catalysts. Thus, in MW-assisted transesterification reactions, all catalysts displayed FAME yields of over 80% even after 5 reactions/activation cycles. Additionally, the influence of FFA content on the catalytic activity was investigated. As a result, in the case of Fe3O4@SiO2-EDG, a higher tolerance towards the FFA content can be noticed with a FAME yield of over 90% (for a 5% (weight) vs oil catalyst content) and 5% weight FFA content.
Experimentelle Studie: Beinwellextrakt fördert die
Regeneration von beschädigter Epidermis
Authors: Dähnhardt Dorothee , Dähnhardt-Pfeiffer Stephan , Groeber-Becker Florian , Fölster-Holst Regina , Schmidt Mathias 
Published: September 2021

Zusammenfassung
Hintergrund Wundheilungseffekte lassen sich im Modell der restrukturierten
humanen Epidermis durch Erzeugung reproduzierbarer Schäden und der
Beobachtung der Regeneration der Hautzellen objektivieren.
Methoden und Ergebnisse In einer kürzlich veröffentlichten
Studie wurde der Effekt einer Creme mit einer Zubereitung aus den oberirdischen
Pflanzenteilen von medizinisch genutztem Beinwell (Symphytum x
uplandicum Nyman) auf künstlich erzeugte Schäden an
der Epidermis mittels Licht- und Elektronenmikroskopie beobachtet. Bei Kontakt
mit Beinwellcreme zeigte sich eine deutliche Beschleunigung der
Regenerationsvorgänge gegenüber nicht-exponierten Kontrollen. Am
7. Tag zeigte sich in den Kontrollproben eine erste Differenzierung neu
gebildeter Zellschichten zu Zellen des Stratum spinosum, in den
Beinwell-behandelten Proben war dagegen dieses Stadium bereits am 4. Tag
erreicht sowie am 7. Tag eine Differenzierung bis zum Stratum corneum.
Schlussfolgerungen Die Untersuchung verfolgte die Frühphase der
Regeneration der Epidermis nach künstlich induzierten Schäden im
Vergleich mit unbehandelten Kontrollen. Die direkte Beobachtung einer
beschleunigten Regeneration der Epidermis steht im Einklang mit der in
kontrollierten Studien klinisch beobachteten schnelleren Abheilung von
Schürfwunden durch medizinischen Beinwellkrautextrakt.
Wirkmechanismen von Duftstoffen und ätherischen Ölen:
Wie beeinflussen sie Emotionen und kognitive Leistungen?
Authors: Heuberger Eva 
Published: September 2021

ZusammenfassungKlinische Studien zur Wirksamkeit von Aromatherapie attestieren
ätherischen Ölen ein großes Potenzial für die
Behandlung von affektiven Beschwerden und kognitiven Defiziten. Nach Jellinek
basiert die psychogene Wirksamkeit von ätherischen Ölen und
Duftstoffen jedoch nicht nur auf pharmakologischen, sondern auch auf
psychologischen Wirkmechanismen, sodass die resultierenden Effekte durch
verschiedene, intrapersonelle Faktoren beeinflusst werden können. In
diesem Beitrag werden die einzelnen Mechanismen vorgestellt und anhand von
Beispielen aus der Emotions- und Kognitionsforschung diskutiert. Der
Zusammenhang zwischen Anwendungsart und Wirksamkeit von ätherischen
Ölen wird ebenso beleuchtet wie ihre möglichen
unerwünschten Wirkungen. Die Kenntnis der verschiedenen Wirkmechanismen
ermöglicht einen gezielten Einsatz von ätherischen Ölen
und kann die Aromatherapie zu einer noch effizienteren phytotherapeutischen
Behandlungsform machen.SchlüsselwörterAromatherapie, Kognition,
Emotion, olfaktorische Stimuli,hedonische Valenz
Cycled Lighting Versus Continuous Near Darkness in Preterm Infants: A Randomized Controlled Trial
Authors: Arayici Sema , Simsek Gulsum Kadioglu , Uras Nurdan , Oncel Mehmet Yekta , Canpolat Fuat Emre , Oguz Serife Suna 
Published: September 2021

ABSTRACT
Objective To compare the effectiveness of cycled lighting (CL) or continuous near darkness (CND) on weight in preterm infants.
Study Design Total 147 infants with a gestational age 25–32 weeks and/or a birth weight 750–1500 g were included in the study. The infants were classified into two groups: CL and CND. Weight on postnatal day 14, weight at corrected 35 weeks, mean rectal temperature and serum growth hormone, cortisol, melatonin levels at 35 weeks’ post menstrual age (PMA), weekly weight gain, full enteral feeding time, duration of mechanical ventilation, retinopathy of prematurity (ROP), length of stay in the hospital, weight and length at three and six months corrected age were compared between the groups.
Results There were no differences between weights on postnatal day 14. Anthropometric parameters and mean rectal temperatures of groups also were not different at 35 weeks’ PMA. There were no differences among the groups in duration of full enteral feeding time, length of stay in hospitalization, duration of mechanical ventilation, ROP and anthropometric parameters. Further, serum growth hormone, cortisol, and melatonin levels were similar between the groups at 35 weeks’ PMA.
Conclusion CL and CND did not have any advantages over each other.
Low Plasma Ascorbate Levels in Type 2 Diabetic Patients With Adequate Dietary Vitamin C
Authors: Bansal Abhishek , Hadimani Chetana P. 
Published: September 2021

Abstract
Background Dietary intake of antioxidative vitamin C plays a protective role in the prevention of oxidative damage in diabetics, demanding increased requirement of vitamin C. Hyperglycemia results in impaired uptake of vitamin C in the cell. The present study was conducted to compare the plasma ascorbate levels in type 2 diabetic patients and controls consuming adequate dietary vitamin C.
Methodology Fifty consented type 2 diabetes mellitus (T2DM) patients who were on treatment with oral hypoglycemic drugs and consuming adequate vitamin C in diet were taken in the study and 50 healthy controls equitably matched for age, gender between 40 and 70 years, and dietary intake of vitamin C were compared. Dietary intake of vitamin C was estimated by a food frequency questionnaire. Subjects consuming more than 35 mg/d of vitamin C were included in the study. Fasting blood sugar was estimated by glucose oxidase and peroxidase method and estimation of ascorbic acid was done by using 2, 4 dinitro phenyl hydrazine method.
Result The mean ± standard deviation levels of plasma ascorbate levels in diabetic subjects were 0.22 ± 0.12 mg/dL, which were significantly lower as compared with controls with plasma ascorbate level of 0.47 ± 0.15 mg/dL. In diabetic subjects, insignificant positive correlation was observed between these parameters with r-value 0.168 and p-value 0.245.
Conclusion This study concludes that even with the recommended dietary intake of vitamin C low plasma ascorbate levels were found among T2DM patients, which necessitates increased demand and dietary advice to diabetic patients on consuming foods rich in vitamin C more than the recommended daily allowance.
Land-use change from food to energy: meta-analysis unravels effects of bioenergy on biodiversity and cultural ecosystem services
Authors: Donnison Caspar , Holland Robert A , Harris Zoe M , Eigenbrod Felix , Taylor Gail 
Published: September 2021

Abstract
Bioenergy has been identified as a key contributor to future energy scenarios consistent with the Paris Agreement targets, and is relied upon in scenarios both with and without bioenergy with carbon capture and storage, owing to the multiple ways in which bioenergy can substitute fossil fuels. Understanding the environmental and societal impacts of land-use change (LUC) to bioenergy crops is important in determining where and how they could be deployed, and the resulting trade-offs and co-benefits. We use systematic review and meta-analysis to assess the existing literature on two poorly understood impacts of this LUC that are likely to have an important effect on public acceptability: cultural ecosystem services and biodiversity. We focus on the impact of LUC to non-food bioenergy crops on agricultural landscapes, where large-scale bioenergy planting may be required. Our meta-analysis finds strong benefits for biodiversity overall (up 75% ± 13%), with particular benefits for bird abundance (+81% ± 32%), bird species richness (+100% ± 31%), arthropod abundance (+52% ± 36%), microbial biomass (+77% ± 24%), and plant species richness (+25% ± 22%), when land moves out of either arable crops or grassland to bioenergy production. Conversions from arable land to energy trees led to particularly strong benefits, providing an insight into how future LUC to non-food bioenergy crops could support biodiversity. There were inadequate data to complete a meta-analysis on the effects of non-food bioenergy crops on cultural ecosystem services, and few generalizable conclusions from a systematic review of the literature, however, findings highlight the importance of landscape context and planting strategies in determining impact. Our findings demonstrate improved farm-scale biodiversity on agricultural land with non-food bioenergy crops, but also limited knowledge concerning public response to this LUC, which could prove crucial to the successful expansion of bioenergy to meet the Paris targets.
The effect analysis of wind speed variation to the horizontal axis wind turbine design with Q-blade
Authors: Samosir A S , Riszal A 
Published: September 2021

Abstract
Wind energy is renewable energy that environmentally friendly. One of the utilizations of wind energy is conversing the wind turbine. The wind turbine is an energy conversion device to convert wind energy into mechanical energy and convert it into electricity through the generator. The size of the power produced by wind turbine depends on the wind speed and the type of turbine that is used. In this research, the researchers design the wind turbine with wind speed variations. The purpose of the designing and the analysing the wind speed is to design a low wind turbine using number of blade 5 model. The objective of this study was to design a wind turbine with variations in wind speed of 2 m/s, 3 m/s, 4 m/s, 5 m/s and 6 m/s using number of blade 5 model wind turbine horizontal axis, rotor diameter of 1.4 m and this rotor used NACA 4412 air foil type. The method that is used in this study is Blade Element Momentum (BEM) and Q-Blade software for designing the simulation. The results of this study, the researchers found there is an increase in power as the wind speed increases.
Coal velocity and proximate analysis relationship using Multiple Linear Regression
Authors: Erfani S , Siregar F M S , Zaenudin A , Rustadi  , Yogi I B S , Wibowo R C 
Published: September 2021

Abstract
Coal properties such a velocity (Vp) are essential to building a lateral distribution of coal seam using seismic data. The experimental determination of velocity analysis is sophisticated, long time consumed, and expensive. On the contrary, statistical approaches such as linear regression can be run rapidly. The study’s two main objectives were to develop models for coal velocity using well log data variables (density and natural Gamma-Ray) and found the relationship between velocity with proximate analysis results. Multiple linear regression (MLR) methods were applied to estimate Vp’s relationship between estimated and proximate analysis. By conducting cross-validation, the prediction analysis of the models has been tested by using R2. The result showed that between Vp estimated versus Vp log have R2 0.80 and Vp estimated versus proximate analysis that reflected have R2 of 0.52. Correlations can estimate the relationship between Vp and proximate analysis, then applied that correlation to distributed in seismic volume to obtain coal seam characteristic.
Investigation and modelling of the flood control system in the Aerotropolis of Yogyakarta International Airport
Authors: Fadilah S , Istiarto  , Legono D 
Published: September 2021

Abstract
The New Yogyakarta International Airport has become one of Indonesia’s National strategic projects and potentially support the development of the Aerotropolis area around the airport. Located on the southern coast of Yogyakarta with rivers at the airport’s upstream, flooding can be a threat to the Aerotropolis area and the Yogyakarta International Airport. This research aims to investigate and model the flood review of the existing design of the flood control system of the Aerotropolis area and find out the possible option of better flood control in the area. The one-dimensional Hydrologic Engineering Center-River Analysis System (HEC-RAS) 4.1 was used to simulate the flood and its control in the Aerotropolis area. Simulation of flood control options includes river normalization, optimization of flood gates, retarding basin, and flood pumps. The present study proposed adjustment of performing in the use of a flood control alternative. Alternative flood control at each flood-prone area was adjusted to the level of flooding risk in the area. In conclusion, the use of retarding basin and flood pumps highly recommended to prevent flooding at the Aerotropolis area of Yogyakarta International Airport.
Optimization of X-ray parameter monitor wireless system based on internet of things
Authors: Triani A B , Budiyanto S , Adriansyah A , Alaydrus M 
Published: September 2021

Abstract
The purpose of this research is to develop a non-invasive X-ray acceptance test device with an internet of things (IoT) based on monitoring system as an interface connection. This experiment uses an application with IoT technology as a graphical user interface for the X-ray acceptance test prototype. The experiment is done by measuring the value of the kilovolt and millisecond on the General X-ray machine. The data result will be compared with the traceable X-ray calibrator as reference value. The overall average percentage of error result is 99.26% and the percentage of accuracy is 0.74%. It is generally concluded that this prototype experiment has good results and can be used as a X-ray acceptance test equipment.
Concentration profile and water level effect on the evaporation in the spent nuclear fuel storage pool using a diffusion approach
Authors: Sundari T , Wahid A 
Published: September 2021

Abstract
In the spent nuclear fuel storage pool (SFSP) of GA Siwabessy Multipurpose Reactor (GAS-MPR), there are three status of pool water level with unknown effect of water level on evaporation rate. This study aims to determine the effect of pool water level on the evaporation rate in the SFSP, so that it can be a consideration in minimizing the evaporation rate. A prediction of water evaporation rate can be explained as a diffusion model through a stagnant gas film. A numerical method, 4th order Runge-Kutta was used to describe the concentration profile of water and air over a SFSP column to the top of the pool. The effect of the water level can be explained then. The various distance of the water to the pool top at constant water temperature and constant air temperature shows a result that the evaporation rate is decreasing while the distance of the water to the pool top is increasing. The investigation results conclude that the closer of the liquid-water surface to the gas-air flow, increases the evaporation rate. This approach can be applied in a SFSP that has an abundant amount of water, the water temperature remained constant by operation of the water-cooling system, air temperature, pressure, and relative humidity remained constant as well by the operation of ventilation and air conditioning (VAC) system. Those constant conditions were stated as normal operation in SFSP of GAS-MPR. In the case of abnormal conditions such as the VAC not operating or the water-cooling system not operating, there will be dynamics of pool water and air temperatures which require further modeling research.
Determine the dynamic load acting on the chassis of multipurpose forest fire fighting vehicle
Authors: Van L V , Tung N T 
Published: September 2021

Abstract
Multi-purpose forest fire fighting vehicle is produced in Viet Nam include a combination of fire fighting equipment such as high-pressure water pump, create corridor fire insulation cutting machine, vacuum and high wind speed bowling machine, extinguish the fire sand blast apparatus that is mounted active three axles vehicle. The paper presents how to determine the dynamic load acting on the chassis by 3D modeling method. The results of this study are the input parameters for assessing the durability of the chassis of multi-purpose forest fire fighting vehicle.