bluuebunny/crossref_metadata_2025_split huggingface dataset:
New MicroRNAs&nbsp;Candidates to Treat Human Colorectal Cancer; Molecular Dynamic Simulations Found a Major Down-Regulator of CLCA4&nbsp;Tumor Suppressor Gene
Authors: Alashti Fariborz Asghari , Goliaei Bahram , Karami Leila , Vassiliev Serguei , Jooyan Najmeh , Minuchehr Zarrin 
Published: April 2021

Abstract
IntroductionColorectal cancer (CRC) is one of the most common malignancies worldwide. The expression of CLCA4, a tumor suppressor gene, decreases significantly in cancer cells of CRC. In this study, we identified miRNAs target the mRNA of the CLCA4 gene. ObjectiveThe aim of this study was the identification of miRNAs involved in CRC.Material and methodsWe predicted miRNA(s) that target CLCA4 mRNA applying TargetScan v.7. Then through analysis of Gene Expression Omnibus (GEO) datasets, among them, miRNA(s) over-expressed in CRC cells were determined. To identify miRNAs with the highest potential to down-regulate CLCA4 through binding, we calculated the binding free energies of the candidate miRNA- mRNA complexes using the molecular mechanics energies combined with several solvation models: The Poisson–Boltzmann (MM/PBSA), the generalized Born (MM/GBSA), and the three-dimensional reference interaction site model with Kovalenko–Hirata closure relation (3D-RISM-KH). ResultsOur TargetScan analysis predicted that 106 miRNAs could bind to CLCA4 3' UTR mRNA. Hsa-miR-934, hsa-miR-574-5p, hsa-miR-377-3p, hsa-miR-5580-3p, hsa-miR-4775, hsa-miR-590-3p and hsa-miR-501-5p showed increased expression in CRC samples compared to normal cells. MD results found the lowest free energy changes in three hsa-miR-377-3p, hsa-miR-574-5p and hsa-miR-501-5p miRNAs. ConclusionThis research beside introducing a new fast and low cost plan to find best candidate of miRNAs to bind their targets, suggested miR-501-5p as a biomarker for early diagnosis of CRC. As well, preventing of down regulation of the CLCA4 expression through interrupting in the expression of miR-574-5p and miR-377-3p and more effectively miR-501-5p probably treat or slow down the development of colorectal cancer.
New and Persistent Controlled Substance Use Among Patients Undergoing Mastectomy and Reconstructive Surgery
Authors: Cogan Jacob C. , Raghunathan Rohit R , Beauchemin Melissa P , Accordino Melissa K , Elkin Elena B , Melamed Alexander , Wright Jason D , Hershman Dawn L 
Published: April 2021

Abstract
PurposeProlonged use of controlled substances can place patients at increased risk of dependence and complications. Women who have mastectomy and reconstructive surgery (M+R) may be vulnerable to becoming new persistent users (NPUs) of opioid and sedative-hypnotic medications.MethodsUsing the MarketScan health care claims database, we identified opioid- and sedative-hypnotic-naïve women who had M+R from 2008-2017. Women who filled ≥1 peri-operative prescription and ≥2 post-operative prescriptions within one year after surgery were classified as NPUs. Univariate and multivariable logistic regression analyses were used to estimate rates of new persistent use and predictive factors. Risk summary scores were created based on the sum of associated factors.ResultsWe evaluated 25,270 opioid-naïve women and 27,651 sedative-hypnotic-naïve women.We found that 18,931 opioid-naïve women filled a peri-operative opioid prescription, and of those, 3,315 (17.5%) became opioid NPUs post-operatively. Additionally, 10,781 sedative-hypnotic-naïve women filled a peri-operative sedative-hypnotic prescription, and of those, 1,837 (17.0%) became sedative-hypnotic NPUs. Development of new persistent sedative-hypnotic use was associated with age ≤49 (OR 1.79 [95% CI 1.43–2.25]) and age 50-64 (1.65 [1.31-2.07]) compared to age ≥65; Medicaid insurance (1.92 [1.23–2.98]); southern residence (1.38 [1.20–1.59]); breast cancer diagnosis (1.78 [1.09–2.91]); and chemotherapy (2.24 [2.02–2.49]). Risk of NPU increased with higher risk score. Women with ≥3 of these risk factors were three times more likely to become sedative-hypnotic NPUs than patients with 0 or 1 factors (3.03 [2.60–3.53]). Comparable findings were seen regarding new persistent opioid use.ConclusionWomen who have M+R are at risk of developing both new persistent opioid and new persistent sedative-hypnotic use. A patient’s risk of becoming an NPU increases as their number of risk factors increases. Non-pharmacologic strategies are needed to manage pain and anxiety following cancer-related surgery.
Synthesis of Biodegradable Aloevera-acrylic Acid Based Hydrogel With Enhanced Water Retention Capacity and Its Impact on Agriculture&nbsp;
Authors: sharma suruchi , Kumar Vaneet 
Published: April 2021

Abstract
The present work deals with the synthesis of biodegradable hydrogel using a natural polysaccharide Aloevera and vinyl monomer acrylic acid. In this synthesis, ammonium per sulphate was used as an initiator and glutaraldehyde as cross-linker, acrylic acid was used as monomers and Aloevera as backbone. Grafting was confirmed by different techniques like SEM, FT-IR, XRD and EDS. Maximum percentage swelling of synthesized hydrogel was found to be 796 %. Biodegradation behavior of Av-cl-poly(AA) was studied by soil burial, compositing and vermicompositing method. Maximum biodegradation was found to be 90%, 94% and 93% in case of soil burial, composting and vermicomposting methods, respectively. Biodegradation of Av-cl-poly(AA) was confirmed by FTIR and SEM techniques. Water retention capacity was prolonged from 11days to 20 days using synthesized Av-cl-poly(AA). Water content of clay soil and sandy loam soil was increased to an extent of 6.1% and 5.79%, respectively.
Cold Stress Activates the UCP3 Pathway via Gut Microbiota in Piglet
Authors: Zhang Yu , Sun Lan , Zhu Run , Zhang Shiyu , Liu Shuo , Wang Yan , Wu Yinbao , Xing Sicheng , Liao Xindi , Mi Jiandui 
Published: April 2021

Abstract
BackgroundThe gut microbiota plays a key role in the host metabolic ﻿thermogenesis by activating ﻿uncoupling protein 1 (UCP1) and increasing the browning process of white adipose tissue, especially in a cold environment. However, the crosstalk between gut microbiota and pigs, which lack functional UCP1 making them susceptible to cold, has rarely been illustrated. We used male piglets as a model to evaluate the host response to cold stress via the gut microbiota. ResultsWe found that host thermogenesis and insulin resistance with increased serum metabolites, such as glycocholic acid (GCA), glycochenodeoxycholate (GCDCA), and chenodeoxycholate (CDCA), and altered cecal microbiota compositions and functions under cold stress. Using transcriptomics technology, we found that ﻿cytochrome P450, family 8, subfamily b, polypeptide 1 (CYP8B1), FXR, FFAR2, and FFAR3, which are related to bile acid and short-chain fatty acid metabolism, increased in the liver under cold stress. In addition, the fat lipolysis genes CLPS, PNLIPRP1, carnitine palmitoyltransferase 1B (CPT1B), and uncoupling protein 3 (UCP3) were significantly increased in the fat of piglets under cold stress. However, microbiota depletion via treatment with mixed antibiotics weakened the effect on the genes CYP8B1, FFAR2, FFAR3, and CPT1B genes, indicating that the gut microbiota plays important roles in the host thermogenesis. ConclusionsOur results demonstrate that the gut microbiota-blood-liver and fat axis may regulate thermogenesis during cold acclimation in piglets.
Reliable H-Infinity Fusion Estimation of Time-Delayed Nonlinear Systems With Energy Constraints: The Finite-Horizon Case
Authors: Xie Meiling , Ding Derui , Wei Guoliang , Yi Xiaojian 
Published: April 2021

Abstract
The fusion estimation issue of sensor networks is investigated for nonlinear time-varying systems with energy constraints, time-delays as well as packet loss. For the addressed problem, some local estimations are first obtained by using the designed Luenberger-type local estimator and then transmitted to a fusion center (FC) to generate a desired fusion value, where two classes of channels, whose schedules are governed by a diagonal matrix, are utilized to perform the information transmission. With the help of the Lyapunov stability theory, sufficient conditions are established to ensure the predetermined local and fused H-infinity performances over a finite horizon. Furthermore, by virtue of the well-known Schur complement lemma, the desired gains of local estimators and the suboptimal fusion weight matrices are obtained in light of the solution of linear matrix inequalities. It should be pointed out that the developed scheme is a two-step process under which the design of fusion weight matrices is based on the obtained estimator gains. Finally, a simulation example for sensor networks is performed to check the effectiveness of the proposed fusion scheme.
Multisource energy conversion modes in minimally altered plants with soft epicuticular coatings
Authors: Meder Fabian , Mondini Alessio , Visentin Francesco , Zini Giorgio , Crepaldi Marco , Mazzolai Barbara 
Published: April 2021

Abstract
Living plants have recently been exploited for unusual tasks such as energy conversion1–6 and environmental sensing.7–12 Yet, using plants as small-scale autonomous energy sources1–5 was obstructed by insufficient power outputs for steadily driving even low-power electronics. Moreover, multicable and -electrode installations on the plants made a realization challenging. Here, we show that plants, by a minimal modification of the leaf epicuticular region and by exploiting their intrinsic circuitry, can be transformed into cable-free, fully plant-enabled integrated systems for multisource energy conversion. In detail, leaf contact electrification caused by wind-induced inter-leaf tangency was magnified by a transparent elastomeric coating on one of two interacting leaves for converting wind energy into harvestable electricity. Further, augmentation of the power output is achieved by coupling multi-frequency band radio frequency (RF) energy conversion modes using the same plant as an unmatched Marconi-antenna. In combination, we observed up to 1100 % enhanced energy accumulation respective to single source harvesting and a single plant like ivy could power a commercial sensing platform wirelessly transmitting environmental data. This shows that living plants could autonomously supply application-oriented electronics while maintaining the positive environmental impact13 by their intrinsic benefits such as O2 production, CO2 fixation, self-repair, and many more extremely difficult (if at all possible) to realize in artificial harvesters.
Clinical Characteristics of Adolescents Undergoing Psychotherapy.
Authors: Tsamadou E. , Voultsos Polychronis , Emmanouilidis A. , Demertzi E. , Ampatzoglou G. 
Published: April 2021

Abstract
Background: Adolescents with mental disorders often have difficulty engaging in ongoing treatment. Drop out from treatment is common.Aim: This paper aims to explore the clinical characteristics of adolescents with mental disorders who were stably and actively undergoing psychotherapy over a long period of time.Method: A single-centre cross-sectional cohort survey was conducted. A sample of fifty participants was recruited from the Child and Adolescent Psychiatry outpatient setting of a tertiary hospital of Thessaloniki, the second largest city in Greece. An intelligence test (Wechsler Intelligence Scale for Children, WISC III) and a self-report measure of depression (Beck Depression Inventory, BDI II) were used. All the participants underwent a rigorous clinical assessment of their mental health status in both initial and ongoing psychotherapy. The initial diagnosis was reconfirmed during the course of therapy. Mental disorders were defined and diagnosed using the ICD-10 (1992) (International Classification of Diseases).Results: The largest percentage of adolescents (44,9%) were found to suffer from mood (affective) disorders, while 20,4% suffered from neurotic disorders. We also high prevalences of pessimism (32,7%), reduction of energy (28,6%) and difficulty in concentration (32,7%). A total of 22,4% of adolescents reported sleep disorders. A limited interest in sex was noted, which was in contrast with international and Greek data, where interest and experimentation around sex seems to preoccupy a high percentage of adolescents. Furthermore, sleep disorders, either as a symptom of an underlying disease or as an independent clinical condition, seem to preoccupy adolescents, and this may be a motive for them to seek treatment.Conclusion: For the most part, the findings of this study were consistent with the findings of prior studies; however, previous studies did not exclusively include adolescents engaging in ongoing psychotherapy. As we identified some inconsistencies with prior studies related to interest in sex and sleep disorders, further research is recommended for the investigation of possible correlations between these findings and ongoing psychotherapy engagement rates.
ARB and ARGs survived from the extremely acidity posing a risk on intestinal bacteria in an in vitro digestion model by horizontal gene transfer
Authors: Cai Qiujie , Xu Yanbin , Zhou Min , Yu Ling , Ouyang Pengqian , Zheng Li 
Published: April 2021

Abstract
Background: Antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) have been considered as emerging contaminants, which even might be closely related to human health.Methods: To investigate the disease-producing risk of ARB and the horizontal gene transfer (HGT) risks of both extracellular ARGs (eARGs) and intracellular ARGs (iARGs), an in vitro digestion model was established to simulate the process of ARB and ARGs going through digestive tract. CTC/DAPI-FCM assay was used to study the survival of ARB during digestion, and the changes of genes (including tetA, tetG, tetM, sul1, sul2, bla EBC, bla FOX, intI1 and 16S rRNA) were determined by QPCR.Results: The results showed that ARB were mostly affected by pH of gastric juice. About 99% ARB (total population of 2.45 × 109–2.54 × 109) were killed by the gastric juice of pH 2.0 for the severely damage of bacterial cell membrane, but more than 80% ARB (total population of 2.71 × 109–3.90 × 109) were still alive with intact cell membrane when the pH of gastric juice increased to 3.0 and above. ARGs, intI1 and 16S rRNA could be detectable even at extreme pH when most bacteria died. The eARGs (accounting for 0.03%–24.56% of total genes) were less than iARGs obviously. The eARGs showed greater HGT potential than that of iARGs, suggesting transformation occurs more easily than conjugation. The transfer potential followed the order as: tet (100%) > sul (75%) > bla (58%), related to the high correlation of intI1 with tetA and sul2 (p < 0.01). Moreover, gastric juice of pH 1.0 could decrease the transfer frequency of ARGs by 2–3 order of magnitude compared to the control, but still threatening human health.Conclusions: Under the treatment of digestive juice, ARGs still have high gene horizontal transfer potential, suggesting that food-borne ARB pose a risk of ARGs horizontal transfer to intestinal bacteria.
Baseline Predictors of Remission, Pain and Fatigue in Rheumatoid Arthritis: The TITRATE Trial
Authors: Lee Sook Yan , Ibrahim Fowzia , Tom Brian , Nikiphorou Elena , Williams Frances , Lempp Heidi , Scott David 
Published: April 2021

Abstract
Background
Clinical trials show intensive treatment to induce remission is effective in patients with highly active rheumatoid arthritis (RA). The TITRATE trial showed that the benefits of intensive treatment also extend to moderately active RA. However, many patients failed to achieve remission or show improvements in pain and fatigue. We investigated whether baseline predictors could identify treatment non-responders.

Methods
The impact of obesity, depression, anxiety and illness perception on RA outcomes, including disease activity, remission, pain and fatigue were determined using a pre-planned secondary analysis of the TITRATE trial data.

Results
Body mass index was associated with disease activity levels and remission: obese patients had a higher overall disease activity and fewer obese patients achieved remission. Intensive management was not associated with increased remission in these patients. Obesity was also associated with increased overall pain and fatigue. Anxiety, depression and health perceptions had no discernible impact on disease activity but were associated with high levels of pain and fatigue. There was a strong association between anxiety and high pain scores; and between depression and high fatigue scores; and health perception was strongly related to both. None of the predictors had an important impact on pain and fatigue reduction in cross-sectional analysis.

Conclusions
Disease activity is higher in obese patients and they have fewer remissions over 12 months. Anxiety, depression and health perceptions were associated with higher pain and fatigue scores. Intensive management strategies need to account for these baseline features as they impact significantly on clinical and psychological outcomes.
Synthesis, DFT calculations, photophysical, photochemical properties of peripherally metallophthalocyanines bearing (2-(benzo[d] [1,3] dioxol-5-ylmethoxy) phenoxy) substituents
Authors: Solğun Derya Güngördü , Yıldıko Ümit , Ağırtaş Mehmet Salih 
Published: April 2021

Abstract
4-(2-(benzo[d] [1, 3] dioxol-5-ylmethoxy) phenoxy) phthalonitrile was first prepared as a starting material. Then, this new phthalonitrile derivative was reacted with Zn and Co salts to obtain new phthalocyanine complexes. Phthalocyanine complexes were evaluated by fluorescence emission, extinction, and absorption measurements. Aggregation studies show the compliance with the lambert-beer law in the concentration range studied for peripheral phthalocyanine compounds. The density functional theory (DFT) calculations of the metallophthalocyanines compounds were performed using B3LYP / 6-31G in Zn-Pc and B3LYP / LanL2DZ in Co-Pc to derive structural optimization, HOMO-LUMO energy parameters, and NLO properties. The calculated values of M-Pcs with different center atoms were obtained close to each other. Molecular electronic surface (MEP) maps of the studied compounds are mapped and discussed. The HOMO-LUMO energy gaps of our compounds studied are around 2.1 eV. The docking studies were performed with the phthalonitrile.
Risk Factors for Moderate-to-severe Postoperative Pain After Percutaneous Nephrolithotomy:A Retrospective Study
Authors: Wu Hao-Tian , Ding Tian-fu , Zhang Huan 
Published: April 2021

Abstract
Backgroud and Objectives: Percutaneous nephrolithotomy (PCNL) is a minimally invasive procedure for removing renal calculus ,but a large number of patients experience acute moderate-to-severe pain despite the analgesia provided.The aim of this study is to explore the risk factors for postoperative pain after percutaneous nephrolithotomy (PCNL), and provides a reference for clinical prevention of postoperative pain after PCNL.Methods: According to the inclusion and exclusion criteria, the clinical data of 331 patients who underwent PCNL in our hospital from September 2019 to February 2021 were retrospectively collected. General anesthesia was induced with propofol, sufentanil and rocuronium and maintained with sevoflurane, remifentanil and rocuronium.Depending on whether the patient had moderate-to-severe postoperative pain, they were divided into mild or no pain group and moderate-to-severe pain group. We collected and analyzed data on age,gender, body mass index (BMI),ASA grading,co-morbidities,number of renal calculus,renal calculus size,degree of hydronephrosis,preoperative urine culture,preoperative creatinine,stone burdens,expansion method,tract diameter,nephrostomy tube,calculi residual,operative position ,puncture time ,number of expanded channels,expanded channel time, duration of surgery,vital signs and VAS pain score.The indicators with statistically significant differences were selected and multivariate logistic regression analysis was carried out to determine the risk factors for postoperative pain. Results: Among 331 patients, 221 had moderate-to-severe pain and the incidence rate was 66.77%. Univariate analysis and multivariate logistic regression analysis showed that independent risk factors for moderate-to-severe pain after PCNL were:number of renal calculus(OR=5.303，P=0.001),stone burdens (OR=7.623，P=0.001),calculi residual(OR=0.2377，P=0.001),duration of surgery (min)(OR=1.024， P=0.001). Conclusions: The main risk factors for pain after PCNL are number of renal calculus,stone burdens ,calculi residual and duration of surgery.
A Comparison of Neonatal Outreach Service Activity Before and During The First UK Wave of SARS-COV-2 in Leicestershire, UK
Authors: Saxena Vikas , Inglis Claire , Mittal Sumit , Fawke Joe 
Published: April 2021

Abstract
Neonatal outreach services provide post discharge support to babies with additional needs and their families. This includes support for nasogastric feeding and supplemental home oxygen use for babies with bronchopulmonary dysplasia (BPD). People potentially at higher risk of serious COVID-19 infection have been encouraged to shield and vulnerable babies requiring supplemental oxygen were in this category. This study reviews the impact of COVID-19 on a tertiary Neonatal service outreach team during the first UK COVID wave and lockdown in March-May 2020 compared to March-May 2019. Outreach service activity was captured on a team activity database including home visits, phone contacts, babies on home oxygen and overnight saturation monitoring. Outreach home visits increased by 33%, telephone contacts increased by 24% and additional support was required by families with 20 virtual clinics instead of 7 face to face clinics. However, emergency department attendances by babies receiving supplemental home oxygen dropped significantly. Emergency department attendances for these babies were over 700% higher pre-Covid than during this Covid lockdown period (1.8 vs 0.25 attendances / baby in home oxygen). It was possible to maintain neonatal outreach support with adapted working including risk assessments and appropriate PPE. Feedback on the neonatal outreach service provision during Covid lockdown was received from 75% families with babies in supplemental home oxygen; 83% of them strongly agreed and 17% agreed that a high quality of care was provided during COVID-19. All respondents were extremely likely to recommend the service to friends and families.