bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Assisted History Match Approach in a Brown Field Saves Thirty Percent in Project Time
Authors: Yusuf Nurudeen Oluwatosin , Silpngarmlers Lynn 
Published: August 2021

Abstract
Reservoir-H sequence, comprising of three reservoirs (H1, H2 & H3) is one of the most complex reservoirs in Niger-delta. With a combined well-count in excess of sixty producers and injectors and a production history of more than fifty-five years, the reservoir has had a history of challenging simulation studies with average water-cut matches resulting in new wells having high water breakthrough from onset.
In the latest effort, an assisted history match using genetic algorithm was employed. This approach is a two-step approach including an identification of all relevant history match parameters for the three reservoirs, followed by a fine-tuning of pressure and saturation history match using genetic algorithm. This approach enabled the identification of aquifer assumptions (architecture and transmissibility) as a critical factor in successfully matching the wells in these reservoirs. In addition to pressure and saturation matches, infill opportunities were further validated by tracking current reservoir fluid contacts with the model.
The current model has significantly improved the overall water-cut match in more than ten wells that historically had water-breakthrough challenges while using principally global history-match parameters. The elimination of many local changes in the current model is expected to improve both the reliability and the shelf of the model. Also, the variance between estimated contacts compared to actual gas-oil and oil-water contacts around infill locations is less than five feet indicating good predictability of the model. In order to save development cost, multiple opportunities identified in these reservoirs are to be targeted with dual strings. Additional savings were realized by reducing the overall simulations studies timeline by four months.
A Pseudo-Radial Pressure Model for Near-Wellbore Condensate Banking Prediction
Authors: Dala Joshua , Akanji Lateef , Bello Kelani , Olafuyi Olalekan , Jadhawar Prashant 
Published: August 2021

Abstract
A new pseudo-radial pressure model for inflow performance analysis and near-wellbore condensate banking deliverability is developed. Analysis of condensate banking and evolution in near wellbore region (i.e. zone 3) has been extensively studied. The new zone 4 region identified in this work will help in delineating the limit of retrograde condensation and the onset of revapourisation. Revapourisation after retrograde condensation is usually not accounted for in most field applications. However, in mature fields such as the Oredo field investigated in this study, revapourisation and near wellbore dynamics play an important role in optimising production from the field. The results of the newly formulated model captured the transient retrograde revapourisation near the wellbore for the well X studied in this work.
Identification of Promising CD8 and CD4 T-cell Epitopes for Peptide Vaccine Formulation Against SARS-CoV-2
Authors: Chakraborty Supriyo , Deb Bornali , Nath Durbba , Monoswita Deboja 
Published: August 2021

Abstract
The novel virus “Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)” has been responsible for the worldwide pandemic causing huge devastation and deaths since December 2019. The disease caused by this virus is known as COVID-19. The present study is based on immunoinformatics approach to develop a multi-epitope loaded peptide vaccine to combat the COVID-19 menace. Here, we have reported the 9-mer CD8 T-cell epitopes and 15-mer CD4 T-cell epitopes, free from glycosylation sites, belonging to three proteins, viz. surface glycoprotein, membrane glycoprotein and envelope protein of this virus. Immunogenicity, aliphatic amino acid, antigenicity and hydrophilicity scores of the predicted epitopes were estimated. In addition, other physicochemical parameters namely net charge, Boman index and amino acid contents were also accounted. Out of all the epitopes, three CD8 T-cell epitopes viz. PDPSKPSKR, DPSKPSKRS and QTQTNSPRR and three CD4 T-cell epitopes viz. ASYQTQTNSPRRARS, RIGNYKLNTDHSSSS and RYRIGNYKLNTDHSS were found to be efficient targets for raising immunity in human against this virus. With the help of our identified potent epitopes, various pharma industries might initiate efforts to incorporate those epitopes with carrier protein or adjuvant to develop a multi-epitope-loaded peptide vaccine against SARS-CoV-2. The peptide vaccines are usually cost effective and therefore, could be administered as a preventive measure to combat the spread of this disease. Proper clinical trials must be conducted prior to the use of identified epitopes as vaccine candidates.
Exosomal miR-331 from Chemoresistant Osteosarcoma Cells induced Chemoresistance through Autophagy
Authors: Zhao Aiqing , Zhao Yanbin , Liu Wanlin , Feng Wei , Xing Wenhua , Zhao Zhenqun , Cui Shuxia , Bai Rui , Meng Chenyang 
Published: August 2021

Abstract
BackgroundOsteosarcoma (OS) is a highly malignant tumor. Improving chemotherapeutic resistance is very important to improve the survival rate of OS. Exosomes and microRNAs (MiRNA) play important roles in the mechanism of chemotherapeutic resistance transmission. More and more researches focus the mechanism of miRNAs carried by exosomes in the transmission of chemotherapeutic resistance of OS. This study focused on exploring the mechanism of exosomal miR-331 in the transmission of chemoresistance in OS.MethodsWe cultured OS drug-resistant cells and extracted exosomes of these cells. The secretion and uptake of exosomes in OS drug-resistant cells and OS cells (OSCs) were confirmed by fluorescence tracking assay and transwell experiments. The differential expression of microRNA-331 (miR-331) in exosomes of OS resistant and OS cells was investigated by RT-PCR. The effects of drug-resistant exosomes on proliferation and migration of OS cells were determined by MTT assay and scratches assay. MDC staining, RT-PCR, and Western blot were used to detect the role of autophagy which regulated by drug-resistant cell-derived exosom-miR-331.ResultsWe found that the expression difference of miR-331 between MG63/CDDP and MG63 was the most significant. Drug resistant OSCs secreted exosomes and were ingested by OSCs, which then promoted OSCs to acquire drug resistance. In addition, exosomes secreted by drug-resistant OSCs promote drug resistance by carrying miRNAs. Interestingly, inhibition of miRNA resulted in reduced drug resistance transmission of exosomes. Finally, we found that the exosomes secreted by drug-resistant OSCs could induce autophagy of OSCs by carrying miR-331, thus making OSCs acquire drug resistance. Inhibition of miR-331 can effectively improve drug resistance of OSCs.ConclusionsChemoresistant OSCs-derived exosomes promote the transmission of drug resistance by carrying miR-331 and inducing autophagy. Inhibition of miR-331 could effectively alleviate drug resistance of OSCs.
Complete Genome Sequence of a Novel Umbra-like Mycovirus From the Plant Pathogenic Fungus Phoma Matteucciicola
Authors: Zhou Jia , Chen Daipeng , Liang Xiaofei , Zhou Siyu , Kang Zhensheng , Zheng Li 
Published: August 2021

Abstract
Here, a novel umbra-like mycovirus, ‘Phoma matteucciicola RNA virus 2’ (PmRV2), isolated from Phoma matteucciicola strain HNQH1 in Hainan province of China, was sequenced and analyzed. The complete genomic sequence of PmRV2 is 3,460 nucleotides (nts) with a GC content of 56.71%. Sequence analysis of PmRV2 indicated that the presence of two noncontiguous open reading frames (ORFs) encoding a hypothetical protein and a RNA-dependent RNA polymerase (RdRp), respectively. PmRV2 contains a metal-binding ‘GDN’ triad in Motif C of RdRp while most + ssRNA mycoviruses contained a ‘GDD’ motif in the same region. Additionally, a BLASTp search showed that the RdRp amino acid sequence of PmRV2 was most closely related to the RdRp of Macrophomina phaseolina umbra-like virus 1 (50.72% identity) and Erysiphe necator umbra-like virus 2 (44.84% identity). Phylogenetic analysis indicated that PmRV2 grouped together with Erysiphe necator umbra-like virus 2 (EnUlV2) within the recently proposed family of ‘Mycotombusviridae’.
Promoting Work Ability With a Wearable Activity Tracker in Working Age Individuals With Hip and/or Knee Osteoarthritis: A Randomized Controlled Trial
Authors: Östlind Elin , Eek Frida , Stigmar Kjerstin , Sant’Anna Anita , Hansson Eva Ekvall 
Published: August 2021

Abstract
Background: Physical activity (PA) can improve work ability and health in individuals with hip and/or knee osteoarthritis (OA). The use of wearable activity trackers (WATs) has been shown to increase PA and improve other health outcomes but little is known concerning their effect on work ability. The objectives in this study was to examine the effect of self-monitoring PA with a WAT on work ability, PA and work productivity among individuals of working age with hip and/or knee OA.Methods: Individuals (n=160) were included and cluster-randomized to a Supported Osteoarthritis Self-management Program (SOASP) with the addition of self-monitoring PA using a commercial WAT for 12 weeks (n=86), or only the SOASP (n=74). Primary outcome was self-reported work ability measured with the Work Ability Index (WAI) and secondary outcomes were self-reported PA measured with the International Physical Activity Questionnaire – Short Form (IPAQ-SF) and work productivity, measured with the Work Productivity and Activity Impairment scale: Osteoarthritis (WPAI:OA) at baseline and after three, six and twelve months. Data was primarily analysed with linear mixed models.Results: Participants with data from baseline and at least one follow-up were included in the analyses (n=124). Linear mixed models showed no statistically significant difference between groups regarding pattern of change in work ability or PA, from baseline to follow-ups. Conclusion: The SOASP together with self-monitoring PA with a WAT did not have any effect on the primary outcome variable work ability. Participants already at baseline had good work ability and were physically active, probably thus reducing the possibility for improvements. Future interventions should target a population with lower work ability and PA-level.Trial registration: ClinicalTrials.gov, NCT03354091. Registered 15/11/2017, https://clinicaltrials.gov/ct2/show/NCT03354091
Evaluation and Characteristic of Modified Ventilator Under Hyperbaric Conditions During Volume-controlled Ventilation
Authors: Wang Cong , Xue Lianbi , Liu Jialong , Chang Liyun , Yu Qiuhong , Liu Yaling , Ren Ziqi , Liu Ying 
Published: August 2021

Abstract
Purpose：The stability of the modified ventilator (Shangrila590, Beijing Aeonmed Company, Beijing, China) was evaluated under hyperbaric conditions during volume-controlled ventilation in this study by Michigan test lung (5601i, Grand Rapids, MI, US).Methods：Experiments were performed inside the multiplace hyperbaric chamber at 1.0, 1.5 and 2.0 atmospheres absolute (ATA). The modified ventilator placed inside the hyperbaric chamber was connected to the test lung. During volume-controlled ventilation (VCV), data for the test lung were collected by a personal computer outside the hyperbaric chamber. The preset tide volume (VTset) of the ventilator (400-1000 ml) and the resistance and compliance of the testing lung were adjusted before the experiments at every ambient pressure. With every test setting, the tide volume (VT), inspiratory airway peak pressure (Ppeak) and minute volume (MV) displayed by the ventilator and the test lung were recorded by the computer. We compared the ventilator and test lung data under 1.0, 1.5 and 2.0 ATA to evaluate the stability of the modified ventilator.Results：The variation in VT in the test lung and the ventilator at different ambient pressures changed within a narrow range, and the differences were statistically significant. In every test setting, changes in the MV of the ventilator were limited and acceptable, with significant differences at different ambient pressures. However, Ppeak increased obviously, as detected by the ventilator and test lung at higher ambient pressure during VCV.Conclusions：The modified Shangrila590 ventilator can work well in a hyperbaric chamber. It can provide relatively stable VT and MV during VCV with VTset from 400 ml to 1000 ml when the ambient pressure increases from 1.0 ATA to 2.0 ATA. The raised ambient pressure will lead to increased gas density, which may result in more airway resistance and higher Ppeak during VCV.
Quorum Sensing Inhibitory Potential and Molecular Docking Studies of Phyllanthus Emblica Phytochemicals Against Pseudomonas Aeruginosae
Authors: Baburam Sharmila , Ramasamy Srinivasan , Shanmugam Gnanendra , Mathanmohun Maghimaa 
Published: August 2021

Abstract
Phyllanthus emblica a traditional medicinal plant that is endowed with curative properties including anti-bacterial, anti-fungal, anti-viral, and analgesic properties. Bacteria make use of cell–cell signaling system known as Quorum sensing (QS) and respond to their own population. In most gram-negative bacteria, the transcriptional regulators belonging to the Lux R protein play a crucial role in the QS mechanism by detecting the presence of signaling molecules known as N-acyl homoserine lactones (AHLs). In this present work, the anti-quorum sensing activity of Phyllanthus emblica was evaluated against Pseudomonas aeruginosa. Anti-quorum sensing efficacy of Phyllanthus emblica was estimated with reference to QS Bio-monitoring strain Chromobacterium violaceum. The binding efficacy of the phytochemicals of Phyllanthus emblica against CviR Protein from Chromobacterium violaceum and LasR Protein from Phyllanthus emblica were studied.
Research on the Coordination Characteristics and Interaction Between Innovation-driven Development and Green Development of the Yangtze River Economic Belt in China
Authors: wang wei , zhou lei , chen wei , Wu chao 
Published: August 2021

Abstract
Innovation-driven development and green development are both important ways to achieve regional sustainable development. Many studies have focused on innovation-driven dynamic factors and green development impact factors, yet most have paid little attention to the relationship between the two types of factors. This study considers the innovation-driven development and green development evaluation systems of 130 cities in the Yangtze River Economic Belt. Through expert group evaluation, the three dimensions of green production, green life and green ecology are selected to represent the green development index. Innovation input, innovation performance, and innovation potential reflect the innovation-driven development index. The entropy TOPSIS method is used to measure the innovation-driven development index and the green development index of 130 cities in the Yangtze River Economic Belt. Then, a coupling coordination evaluation model and a spatiotemporal heterogeneity analysis model are constructed to discuss the coupling coordination index of regional innovation-driven development and green development in the Yangtze River Economic Belt and to determine its temporal and spatial distribution characteristics. Finally, we choose a spatial panel regression model to explore the relationship between the innovation-driven development index and the green development index of the Yangtze River Economic Belt. The research results show that there is a significant difference between the innovation-driven development index and the green development index of the 130 cities in the Yangtze River Economic Belt in terms of the temporal and spatial distribution. The coordination index of the two has an imbalanced distribution feature, and there is a significant direct or indirect relationship between the two structural indicators in a mathematical sense. This study enhances the academic community's understanding of the interaction between innovation-driven development and green development, provides scientifically based support for green development, offers guidance for the implementation of innovation capabilities, and ultimately supports a policy design facilitating regional sustainable development.
Fabrication of CdS/Ti3C2/g-C3N4NS Z-Scheme Composites with Enhanced Visible-Light-Driven Photocatalytic Activity
Authors: Zhang Ranran , Jin Jiaying , Jia Lumeng , Shi Bo , Chen Rufen 
Published: August 2021

Abstract
The Ti3C2 and g-C3N4NS were obtained first, and the CdS/Ti3C2/g-C3N4NS Z-scheme composites were prepared via a facile hydrothermal synthesis and their photocatalytic properties were investigated. The g-C3N4NS with a high surface area displayed higher adsorption and degradation capacity. Compared with Ti3C2/g-C3N4NS and CdS, the optimal ternary composites exhibited significantly enhanced visible light (vis) photocatalytic activity. The Fabrication of CdS/Ti3C2/g-C3N4NS Z-scheme heterostructure not only enhanced vis absorption, but also improved the separation ability of the photoinduced e−-h+ pairs and the redox ability of the catalyst, thereby leading to the improvement of visible-light-driven photocatalytic activity. This finding provides new insights into the construction of high efficiency Z-scheme heterostructure photocatalyst.
The LPA-CDK5-Tau Pathway Mediates Neuronal Injury in an In Vitro Model of Ischemia-Reperfusion Insult
Authors: Wang Yaya , Zhang Jie , Huang Liqin , Mo Yanhong , Wang Changyu , Li Yiyi , Zhang Yangyang , Zhang Zhaohui 
Published: August 2021

Abstract
Lysophosphatidic acid (LPA) is a common glycerol phospholipid and an important extracellular signaling molecule. LPA binds to its receptors and mediates a variety of biological effects, including the pathophysiological process underlying ischemic brain damage and traumatic brain injury. However, the molecular mechanisms mediating the pathological role of LPA are not clear. Here, we found that LPA activates cyclin-dependent kinase 5 (CDK5). CDK5 phosphorylates tau, which leads to neuronal cell death. Inhibition of LPA production or blocking its receptors reduced the abnormal activation of CDK5 and phosphorylation of tau, thus reversing the death of neurons. Our data indicate that the LPA-CDK5-Tau pathway plays an important role in the pathophysiological process after ischemic stroke. Inhibiting the LPA pathway may be a potential therapeutic target for treating ischemic brain injury.
Obtaining a Real Natural Numerical Landslide Model by Comparing Measurement and Predicted Values: A Case Study of Kavşakbendi̇ Dam Left Bank Andirap Landslide
Authors: Pamuk Resul , Eröz Murat , Tuncer Kemal , Kılıç Havvanur 
Published: August 2021

Abstract
The Andırap landslide is located on the left bank of the Kavşakbendi dam approximately 50 km from the Kozan district of Adana province in Turkey. After it was determined that the mass stability was impaired during the dam construction work, the landslide movements were followed by surface geodetic measurements and inclinometers. According to the measurements that were taken, displacements totaling 0.10 m were measured between 2010 and 2017, and it was determined that the speed of movement slowed considerably between 2017 and 2020. In this study, the results of stress-strain and stability analyses were evaluated taking into account the soil model created based on extensive site and laboratory research to examine the sliding mechanism of the Andırap landslide mass. After the numerical model was verified using site measurements, the movements of the landslide mass were examined by numerical analyses, taking into account the different loading conditions that may be encountered during the service life of the dam. According to the results of the analysis, no global slide was observed for the slip circle of the Andırap landslide and in the analyses conducted for the situation where the reservoir is full, the deep displacement of 0.11 m was consistent with the average displacement of 0.04 and 0.11 m deformation values measured from inclinometers. In the analyses carried out for the loading condition featuring a full reservoir and earthquake effects, it was calculated that shallow displacements reached up to 1.0 m, but deep displacements were 0.13 m.
In Vitro-in Vivo Digestibility and Nitrogen Balance in Indigenous, Exotic and Crossbred Growing Pigs Fed Sprouted or Roasted Cowpea Diets
Authors: Lubisi Mfanuzile Welcome , Fushai F , Thomas R.S , Baloyi J.J. 
Published: August 2021

Abstract
The study evaluated effects of processing cowpeas for inclusion in maize-based diets for Windsnyer, Large-White x Landrace, and their 3-way crossbred growing pig genotypes. In-vitro, the raw and roasted cowpea diets, sprouting cowpeas decreased (P<0.05) gastric-ileal non-enzymatic (buffer-only) DM digestibility. Roasting increased (P<0.05) the colon enzymatic digestion relative to sprouting. Total ileal and colon in-vitro diet DM digestibility were not affected (P>0.05) by cowpea processing. In-vivo, Pigs consumed most (P<0.05) feed (g/day/kg BW) in period 1, with significant (P<0.05) genotype X period interaction. Both roasting and sprouting cowpeas reduced (P<0.05) dietary apparent DM digestibility. Pig daily body weight (BW) gain reduced (P<0.05) in period 3 compared to period 1. There was no (P>0.05) treatment effect on feed conversion efficiency. The 3-way crossbred pigs excreted more (P<0.05) urine N (g/dayBW0.75). Urine N excretion (g/dayBW0.75) peaked (P<0.05) in period 2 (P<0.05), with less (P<0.05) N intake (g/dayBW0.75), faecal N excretion (g/dayBW0.75) and N balance (g/dayBW0.75) than in period 3. Significant diet X genotype interaction in faecal N excretion (g/dayBW0.75) resulted from markedly high (P<0.05) in contrast to low (P<0.05) excretion. Significant genotype X period interaction resulted from the numerically (P>0.05) higher urine N excretion. In conclusion, in-vitro, sprouting shifted non-enzymatic digestion to the colon, while roasting increased colon fibrolysis, without effect on overall DM digestibility. In-vivo, the period of feeding, interpreted to reflect pig maturity, the pig genotype and cowpea processing interacted to influence apparent dietary DM digestibility and N utilization, without significant effect on the conversion efficacy of the maize-cowpea diet.
Diagnostic and Prognostic Value of Cell-Surface Vimentin-Positive Circulating Tumor Cells in Pediatric Sarcoma Patients
Authors: Dao Long , Ragoonanan Dristhi , Batth Izhar , Satelli Arun , Foglesong Jessica , Wang Jian , Zaky Wafik , Gill Jonathan , Liu Diane , Albert Aisha , Gordon Nancy , Huh Winston , Harrison Douglas , Herzog Cynthia , Kleinerman Eugenie , Gorlick Richard , Daw Najat , Li Shulin 
Published: August 2021

Abstract
Background

Despite advances in care, the 5 year overall survival for patients with relapsed and or metastatic sarcoma remains as low as < 35%. Currently, there are no biomarkers available to assess disease status in patients with sarcomas and as such, disease surveillance remains reliant on serial imaging which increases the risk of secondary malignancies and heightens patient anxiety.
Methods

Here, for the first time reported in the literature, we have enumerated the cell surface vimentin (CSV+) CTCs in the blood of 92 sarcoma pediatric and adolescent and young adult (AYA) patients as a possible marker of disease.
Results

We constructed a ROC with an AUC of 0.831 resulting in a sensitivity of 85.3% and a specificity of 75%. Additionally, patients who were deemed to be CSV + CTC positive were found to have a worse overall survival compared to those who were CSV + CTC negative. We additionally found the use of available molecular testing increased the accuracy of our diagnostic and prognostic tests.
Conclusions

Our findings indicate that CSV + CTCs have both diagnostic and prognostic value and can possibly serve as a measure of disease burden.