bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calibrating the Amplitude and Phase Imbalances in AgileDARN HF Radar
Authors: Deng X. , Lan A. L. , Yan J. Y. , Wu L. , Wu J. , Zhang J. J. , Qiu H. B. 
Published: April 2021

AbstractThe Agile Dual Auroral Radar Network (AgileDARN) radar has been developed as an all‐digital high‐frequency (HF) coherent scatter radar. Based on phased array antenna and digital signal processing techniques, digital beam forming (DBF) is employed for both transmission and reception, which steers toward the desired direction based on the relative phases within the array elements. However, the amplitude and phase imbalances among channels will lead to substantial degradation of DBF performance, including the beam direction deviation, broadening of the main lobe, deteriorated side lobes, antenna gain degradation, and ultimately affects the radar detection accuracy. Here, we present a complete method for calibrating these imbalances in transceivers and linear array elements in AgileDARN radar: internal calibration and external calibration. During the internal calibration, the calibration signals with the same amplitude and phase as the multi‐pulse sequence are sent to all transceivers internally. The variance of the outputs illustrates the transceivers' imbalances and can be processed to implement calibration. Further, the meteor echoes and linear least squares fitting method are employed for external calibration, which calibrates the amplitude and phase imbalances among the cables and phased array elements. The technique has been successfully implemented, and we present the results of measured data observed by the AgileDARN HF radar before and after calibration.
Under‐Ice Dissolved Oxygen and Metabolism Dynamics in a Shallow Lake: The Critical Role of Ice and Snow
Authors: Huang Wenfeng , Zhang Zheng , Li Zhijun , Leppäranta Matti , Arvola Lauri , Song Shuang , Huotari Jussi , Lin Zhanju 
Published: April 2021

AbstractInteraction of under‐ice physical, chemical, and biological processes with lake ice/snow cover is examined to better understand how changing winter climate may affect lake ecosystems. We derived under‐ice dissolved oxygen (DO) dynamics from high‐frequency observations and modified a widely used lake metabolism model by including the effect of freezing and thawing on DO concentration. Estimates were produced for the production and respiration in a shallow lake on the Mongolian Plateau in three winters. Diel, synoptic, and seasonal variations in DO concentration were detected as responses to solar radiation, episodic snowfall events, and occasional convective mixing. Based on the observations and a radiative transfer model, incident solar radiation was partitioned into reflectance, absorbance, and transmittance by the snow and ice cover. For bare ice, the contributions of these three parts were 35%, 39%, and 26%, respectively, while under a new 4.5 cm thick snow cover, the corresponding values were 79%, 17%, and 3%. This points out the critical role of snow and ice on under‐ice light conditions, which is the primary forcing for the temperature and the rate of photosynthesis under ice. The results showed three principal factors, which influenced under‐ice DO and metabolism: (1) thickness and optical properties of ice and snow, which affected the light transfer and depth of the euphotic zone, (2) mediated radiation and ice‐water heat transfer which controlled water temperature, and (3) DO exclusion during freezing and dilution by melt water. This study highlights the ecosystem characteristics in shallow ice‐covered lakes in arid temperate regions and promotes our understanding of the response of the cold aquatic environment to climate change.
Development and Validation of an Empirical Ocean Color Algorithm with Uncertainties: A Case Study with the Particulate Backscattering Coefficient
Authors: McKinna Lachlan I. W. , Cetinić Ivona , Werdell P. Jeremy 
Published: April 2021

AbstractWe explored how algorithm (model) and in situ measurement (observation) uncertainties can effectively be incorporated into empirical ocean color model development and assessment. In this study we focused on methods for deriving the particulate backscattering coefficient at 555 nm, bbp(555) (m−1). We developed a simple empirical algorithm for deriving bbp(555) as a function of a remote sensing reflectance line height (LH) metric. Model training was performed using a high‐quality bio‐optical dataset that contains coincident in situ measurements of the spectral remote sensing reflectances, Rrs(λ) (sr−1), and the spectral particulate backscattering coefficients, bbp(λ). The LH metric used is defined as the magnitude of Rrs(555) relative to a linear baseline drawn between Rrs(490) and Rrs(670). Using an independent validation dataset, we compared the skill of the LH‐based model with two other models. We used contemporary validation metrics, including bias and mean absolute error (MAE), that were corrected for model and observation uncertainties. The results demonstrated that measurement uncertainties do indeed impact contemporary validation metrics such as mean bias and MAE. Zeta‐scores and z‐tests for overlapping confidence intervals were also explored as potential methods for assessing model skill.
Cenozoic Tectonic Deformation Along the Pontarlier Strike‐Slip Fault Zone (Swiss and French Jura Fold‐and‐Thrust Belt): Insights From Paleostress and Geomorphic Analyses
Authors: Radaideh Omar M. A. , Mosar Jon 
Published: April 2021

AbstractWe present insights into the Cenozoic tectonic evolution of the region around the Pontarlier strike‐slip fault zone within the Jura fold‐and‐thrust belt by combined study of paleostress and geomorphic analyses. A preliminary separation of heterogeneous fault‐slip data and bedding‐tilt correction was performed before determining the paleostress axes orientations. The paleostress results provide evidence of multiphase deformation history consisting of four successive events. These events include a strike‐slip stress regime with ∼N‐S directed compression, a NW‐SE directed extensional stress regime, a NW‐SE trending compressional stress regime, and a strike‐slip stress regime with a mean NW‐SE directed compression. The directions of extension and compression for the second and third events are consistent with the general direction of maximum horizontal far‐field stress near the northern segment of the Pontarlier fault and generally deviate from those found near the southern segment.Geomorphic analysis of selected rivers along the southern segment of the Pontarlier fault, together with sub/surface geological observations provides new clues on Late Cenozoic strike‐slip reactivation of inherited extensional structure of Mesozoic age. These clues include (i) anomalous zones of high gradient within uniform bedrock lithology that spatially coincide with an obvious change in the depth to top Mesozoic layers; and (ii) right‐lateral offset of river channels, which is consistent with NW‐SE compression related to the youngest paleostress and current stress field. A combination of paleostress and geomorphic analyses provides a useful complementary approach for unraveling the Cenozoic tectonic evolution of the region characterized by a general lack of outcrop of Cenozoic rocks.
DGG-100629 inhibits lung cancer growth by suppressing the NFATc1/DDIAS/STAT3 pathway
Authors: Im Joo-Young , Kim Bo-Kyung , Yoon Sung-Hoon , Cho Byoung Chul , Baek Yu Mi , Kang Mi-Jung , Kim Nayeon , Gong Young-Dae , Won Misun 
Published: April 2021

AbstractDNA damage-induced apoptosis suppressor (DDIAS) promotes the progression of lung cancer and hepatocellular carcinoma through the regulation of multiple pathways. We screened a chemical library for anticancer agent(s) capable of inhibiting DDIAS transcription. DGG-100629 was found to suppress lung cancer cell growth through the inhibition of DDIAS expression. DGG-100629 induced c-Jun NH(2)-terminal kinase (JNK) activation and inhibited NFATc1 nuclear translocation. Treatment with SP600125 (a JNK inhibitor) or knockdown of JNK1 restored DDIAS expression and reversed DGG-100629-induced cell death. In addition, DGG-100629 suppressed the signal transducer and activator of transcription (STAT3) signaling pathway. DDIAS or STAT3 overexpression restored lung cancer cell growth in the presence of DGG-100629. In a xenograft assay, DGG-100629 inhibited tumor growth by reducing the level of phosphorylated STAT3 and the expression of STAT3 target genes. Moreover, DGG-100629 inhibited the growth of lung cancer patient-derived gefitinib-resistant cells expressing NFATc1 and DDIAS. Our findings emphasize the potential of DDIAS blockade as a therapeutic approach and suggest a novel strategy for the treatment of gefitinib-resistant lung cancer.
Comparison of the acute metabolic effect of different infant formulas and human milk in healthy adults: a randomized trial
Authors: Shahkhalili Yasaman , Monnard Cathriona , Grathwohl Dominik , Sauser Julien , Beaumont Maurice , Zufferey Corinne Ammon , Macé Katherine 
Published: April 2021

AbstractBackground/ObjectivesDifferent infant formulas, varying in protein type and quantity, are available for infants who are not breastfed or are partially breastfed. Postprandial insulinemic and glycemic responses to intact vs partially hydrolyzed protein in infant formula are unclear. To compare the effect of different forms (partially hydrolyzed vs non-hydrolyzed) and levels of protein in infant formula compared with a human milk reference subgroup on insulin response in adults.Subjects/MethodsIn a randomized, double-blinded, cross-over study, 35 healthy adults consumed 600 ml of three different infant formulas: Intact protein-based formula (INTACT) (1.87 g protein/100 kcal; whey/casein ratio of 70/30; 63 kcal/100 ml), partially hydrolyzed whey-based formula (PHw) (1.96 g protein/100 kcal; 100% whey; 63 kcal/100 ml), a high-protein partially hydrolyzed whey-based formula (HPPHw) (2.79 g protein/100 kcal; 100%whey; 73 kcal/100 ml) and a subgroup also consumed human milk (HM) (n = 11). Lipid and carbohydrate (lactose) contents were similar (5.1–5.5 and 10.5–11.6 g/100 kcal, respectively). Venous blood samples were taken after overnight fasting and at different intervals for 180 min post-drink for insulin, glucose, blood lipids, GLP-1, glucagon, and C-peptide.ResultsTwenty-nine subjects (eight consuming HM) adhered to the protocol. INTACT and PHw groups had similar postprandial insulinemia and glycaemia (Cmaxand iAUC) that were not different from those of the HM subgroup. HPPHw resulted in higher postprandial insulin responses (iAUC) relative to all other groups (p < 0.001,p < 0.001,p = 0.002 for the comparison with INTACT, PHw, HM, respectively). HPPHw resulted in a higher glucose response compared to INTACT and PHw (iAUC:p = 0.003,p = 0.001, respectively), but was not different from HM (p = 0.41).ConclusionThis study in adults demonstrates similar postprandial insulinemia and glycaemia between INTACT and PHw, close to that of HM, but lower than HPPHw, which had a higher protein content compared to the other test milks. The findings remain to be confirmed in infants.Clinical trial registrationThis study is registered at clinicaltrials.gov, identifier NCT04332510.
Malignant pleural mesothelioma co-opts BCL-XL and autophagy to escape apoptosis
Authors: Xu Duo , Liang Shun-Qing , Yang Zhang , Yang Haitang , Bruggmann Rémy , Oberhaensli Simone , Berezowska Sabina , Marti Thomas M. , Hall Sean R. R. , Dorn Patrick , Kocher Gregor J. , Schmid Ralph A. , Peng Ren-Wang 
Published: April 2021

AbstractEscape from programmed cell death is a hallmark of cancer. In this study, we investigated the anti-apoptotic mechanisms and explored the therapeutic potential of BCL-2 homology domain-3 (BH3) mimetics in malignant pleural mesothelioma (MPM), a lethal thoracic malignancy with an extreme dearth of treatment options. By implementing integrated analysis of functional genomic data of MPM cells and quantitative proteomics of patients’ tumors, we identified BCL-XL as an anti-apoptotic driver that is overexpressed and confers an oncogenic dependency in MPM. MPM cells harboring genetic alterations that inactivate the NF2/LATS1/2 signaling are associated with increased sensitivity to A-1155463, a BCL-XL-selective BH3 mimetic. Importantly, BCL-XL inhibition elicits protective autophagy, and concomitant blockade of BCL-XL and autophagic machinery with A-1155463 and hydroxychloroquine (HCQ), the US Food and Drug Administration (FDA)-approved autophagy inhibitor, synergistically enhances anti-MPM effects in vitro and in vivo. Together, our work delineates the molecular basis underlying resistance to apoptosis and uncovers an evasive mechanism that limits response to BH3 mimetics in MPM, suggesting a novel strategy to target this aggressive disease.
FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress
Authors: Jang Haeyeon , Jun Yukyung , Kim Suyeon , Kim Eunjeong , Jung Yeonjoo , Park Byung Jo , Lee Jinseon , Kim Jhingook , Lee Sanghyuk , Kim Jaesang 
Published: April 2021

AbstractIn this study, we report a novel function of FCN3 (Ficolin 3), a secreted lectin capable of activating the complement pathway, as a tumor suppressor of lung adenocarcinoma (LUAD). First, the expression of FCN3 was strongly down-regulated in cancer tissues compared to matched normal lung tissues, and down-regulation of FCN3 was shown to be significantly correlated with increased mortality among LUAD patients. Interestingly, while ectopic expression of FCN3 led to cell cycle arrest and apoptosis in A549 and H23 cells derived from LUAD, the secreted form of the protein had no effect on the cells. Rather, we found evidence indicating that activation of the unfolded protein response from endoplasmic reticulum (ER) stress is induced by ectopic expression of FCN3. Consistently, inhibition of ER stress response led to enhanced survival of the LUAD cells. Of note, the fibrinogen domain, which is not secreted, turned out to be both necessary and sufficient for induction of apoptosis when localized to ER, consistent with our proposed mechanism. Collectively, our data indicate that FCN3 is a tumor suppressor gene functioning through induction of ER stress.
The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells
Authors: Gao Wenwen , Huang Mengqiu , Chen Xi , Chen Jianping , Zou Zhiwei , Li Linlin , Ji Kaiyuan , Nie Zhirui , Yang Bingsheng , Wei Zibo , Xu Pengfei , Jia Junshuang , Zhang Qianbing , Shen Hongfen , Wang Qianli , Li Keyi , Zhu Lingqun , Wang Meng , Ye Shuangyan , Zeng Sisi , Lin Ying , Rong Zhili , Xu Yang , Zhu Peng , Zhang Hui , Hao Bingtao , Liu Qiuzhen 
Published: April 2021

AbstractOne of the malignant transformation hallmarks is metabolism reprogramming, which plays a critical role in the biosynthetic needs of unchecked proliferation, abrogating cell death programs, and immunologic escape. However, the mechanism of the metabolic switch is not fully understood. Here, we found that the S-nitrosoproteomic profile of endogenous nitrogen oxide in ovarian cancer cells targeted multiple components in metabolism processes. Phosphofructokinase (PFKM), one of the most important regulatory enzymes of glycolysis, was S-nitrosylated by nitric oxide synthase NOS1 at Cys351. S-nitrosylation at Cys351 stabilized the tetramer of PFKM, leading to resist negative feedback of downstream metabolic intermediates. The PFKM-C351S mutation decreased the proliferation rate of cultured cancer cells, and reduced tumor growth and metastasis in the mouse xenograft model. These findings indicated that S-nitrosylation at Cys351 of PFKM by NOS1 contributes to the metabolic reprogramming of ovarian cancer cells, highlighting a critical role of endogenous nitrogen oxide on metabolism regulations in tumor progression.
Alternative carbon price trajectories can avoid excessive carbon removal
Authors: Strefler Jessica , Kriegler Elmar , Bauer Nico , Luderer Gunnar , Pietzcker Robert C. , Giannousakis Anastasis , Edenhofer Ottmar 
Published: April 2021

AbstractThe large majority of climate change mitigation scenarios that hold warming below 2 °C show high deployment of carbon dioxide removal (CDR), resulting in a peak-and-decline behavior in global temperature. This is driven by the assumption of an exponentially increasing carbon price trajectory which is perceived to be economically optimal for meeting a carbon budget. However, this optimality relies on the assumption that a finite carbon budget associated with a temperature target is filled up steadily over time. The availability of net carbon removals invalidates this assumption and therefore a different carbon price trajectory should be chosen. We show how the optimal carbon price path for remaining well below 2 °C limits CDR demand and analyze requirements for constructing alternatives, which may be easier to implement in reality. We show that warming can be held at well below 2 °C at much lower long-term economic effort and lower CDR deployment and therefore lower risks if carbon prices are high enough in the beginning to ensure target compliance, but increase at a lower rate after carbon neutrality has been reached.
Wobble tRNA modification and hydrophilic amino acid patterns dictate protein fate
Authors: Rapino Francesca , Zhou Zhaoli , Roncero Sanchez Ana Maria , Joiret Marc , Seca Christian , El Hachem Najla , Valenti Gianluca , Latini Sara , Shostak Kateryna , Geris Liesbet , Li Ping , Huang Gang , Mazzucchelli Gabriel , Baiwir Dominique , Desmet Christophe J. , Chariot Alain , Georges Michel , Close Pierre 
Published: April 2021

AbstractRegulation of mRNA translation elongation impacts nascent protein synthesis and integrity and plays a critical role in disease establishment. Here, we investigate features linking regulation of codon-dependent translation elongation to protein expression and homeostasis. Using knockdown models of enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), we show that gene codon content is necessary but not sufficient to predict protein fate. While translation defects upon perturbation of U34-enzymes are strictly dependent on codon content, the consequences on protein output are determined by other features. Specific hydrophilic motifs cause protein aggregation and degradation upon codon-dependent translation elongation defects. Accordingly, the combination of codon content and the presence of hydrophilic motifs define the proteome whose maintenance relies on U34-tRNA modification. Together, these results uncover the mechanism linking wobble tRNA modification to mRNA translation and aggregation to maintain proteome homeostasis.
Millennial scale feedbacks determine the shape and rapidity of glacial termination
Authors: Barker Stephen , Knorr Gregor 
Published: April 2021

AbstractWithin the Late Pleistocene, terminations describe the major transitions marking the end of glacial cycles. While it is established that abrupt shifts in the ocean/atmosphere system are a ubiquitous component of deglaciation, significant uncertainties remain concerning their specific role and the likelihood that terminations may be interrupted by large-amplitude abrupt oscillations. In this perspective we address these uncertainties in the light of recent developments in the understanding of glacial terminations as the ultimate interaction between millennial and orbital timescale variability. Innovations in numerical climate simulation and new geologic records allow us to highlight new avenues of research and identify key remaining uncertainties such as sea-level variability.
Global connections between El Nino and landslide impacts
Authors: Emberson Robert , Kirschbaum Dalia , Stanley Thomas 
Published: April 2021

AbstractEl Nino is a critical part of global inter-annual climate variability, and the intensity of El Nino has major implications for rainfall-induced natural hazards in many vulnerable countries. The impact of landslides triggered by rainfall is likely to be modulated by the strength of El Nino, but the nature of this connection and the places where it is most relevant remains unconstrained. Here we combine new satellite rainfall data with a global landslide exposure model to show that El Nino has far-reaching effects on landslide impacts to people and infrastructure. We find that the impact of El Nino on landslide exposure can be greater in parts of Southeast Asia and Latin America than that due to seasonal rainfall variability. These findings improve our understanding of hazard variability around the world and can assist disaster mitigation efforts on seasonal timescales.
Reconstitution of contractile actomyosin rings in vesicles
Authors: Litschel Thomas , Kelley Charlotte F. , Holz Danielle , Adeli Koudehi Maral , Vogel Sven K. , Burbaum Laura , Mizuno Naoko , Vavylonis Dimitrios , Schwille Petra 
Published: April 2021

AbstractOne of the grand challenges of bottom-up synthetic biology is the development of minimal machineries for cell division. The mechanical transformation of large-scale compartments, such as Giant Unilamellar Vesicles (GUVs), requires the geometry-specific coordination of active elements, several orders of magnitude larger than the molecular scale. Of all cytoskeletal structures, large-scale actomyosin rings appear to be the most promising cellular elements to accomplish this task. Here, we have adopted advanced encapsulation methods to study bundled actin filaments in GUVs and compare our results with theoretical modeling. By changing few key parameters, actin polymerization can be differentiated to resemble various types of networks in living cells. Importantly, we find membrane binding to be crucial for the robust condensation into a single actin ring in spherical vesicles, as predicted by theoretical considerations. Upon force generation by ATP-driven myosin motors, these ring-like actin structures contract and locally constrict the vesicle, forming furrow-like deformations. On the other hand, cortex-like actin networks are shown to induce and stabilize deformations from spherical shapes.
Real-world stress resilience is associated with the responsivity of the locus coeruleus
Authors: Grueschow Marcus , Stenz Nico , Thörn Hanna , Ehlert Ulrike , Breckwoldt Jan , Brodmann Maeder Monika , Exadaktylos Aristomenis K. , Bingisser Roland , Ruff Christian C. , Kleim Birgit 
Published: April 2021

AbstractIndividuals may show different responses to stressful events. Here, we investigate the neurobiological basis of stress resilience, by showing that neural responsitivity of the noradrenergic locus coeruleus (LC-NE) and associated pupil responses are related to the subsequent change in measures of anxiety and depression in response to prolonged real-life stress. We acquired fMRI and pupillometry data during an emotional-conflict task in medical residents before they underwent stressful emergency-room internships known to be a risk factor for anxiety and depression. The LC-NE conflict response and its functional coupling with the amygdala was associated with stress-related symptom changes in response to the internship. A similar relationship was found for pupil-dilation, a potential marker of LC-NE firing. Our results provide insights into the noradrenergic basis of conflict generation, adaptation and stress resilience.