bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy Reduction Potential of Biomimetic Building Facades for Different Building Typologies in Different Climate Zones
Authors: Webb Matthew 
Published: June 2021

Abstract
Greenhouse gas (GHG) emissions leading to anthropogenic global warming continue to be a major issue for societies worldwide. One opportunity to reduce emissions is to improve the effectiveness of building envelope, leading to a decrease in operational energy consumption. Improving the performance of a building's thermal envelope can substantially reduce energy consumption from heating, ventilation and air conditioning while maintaining occupant comfort. In previous work, a computational model of a biomimetic building façade design was found to be effective in temperate climates in an office context. This paper tests the hypothesis that biomimetic building facades have a broader application through a case study example based on animal fur and blood perfusion. Using mathematical modelling and digital simulation methods, the energy reduction potential of the biomimetic façade was tested in a set of operational applications (office, school and aged care) and across different climate zones (tropical, desert, temperate, and cool continental). Results indicated that the biomimetic façade has potential to reduce energy consumption for all building applications, with the greatest benefit shown in residential aged care (67.1% reduction). Similarly, the biomimetic building façade showed potential to reduce operational services energy consumption in all climate zones, with the greatest energy reductions achieved in the tropical (55.4% reduction) and humid continental climates (55.1% reduction). Through these results the hypothesis was confirmed, suggesting that facades engineered to mimic biological functions and processes can improve substantially decrease building operational energy consumption and can be applied in different building classifications and different climate zones. Such facades can contribute to the further reduction of greenhouse gas emissions in a broad range of contexts. This study also exemplified a method by which other biomimetic building envelope features may be assessed. Further work is suggested to assess economic viability and constructability of the proposed facades.
Delineation of Cumin (Cuminum Cyminum L.) Production Zone Beyond Boundaries in India Based on Climate Analogues, Soil Suitability and Rainfall Analysis
Authors: KS Krishnamurthy , K Kandiannan , Sudhakaran Swetha , P Muhammed Bin Faras U , M Alagupalamuthirsolai , K Jayarajan 
Published: June 2021

Abstract
Here, we attempted to identify ideal ecological niche for cumin cultivation in India in the future climate scenario by adopting an integrated approach of climate analogues and soil suitability. This will help policy makers for area expansion to generate more revenue as cumin has huge export potential. Climate analogues tool, a web-based tool developed by the Research Programme on climate change, agriculture and food security (CCAFS) helps to identify, connect and map sites with statistically similar climates across space and time. Efficient cumin production zones in India were identified based on relative spread index and relative yield index which were used as the input/reference site for finding the analogues sites. Rainfall and temperature were the main climatic variables used in the study under SRES A1B emission scenario. The results showed a total of 453 analogues sites (districts) identified by 14 reference sites used in the study. The analogues sites identified by the tool were further corroborated with the soil suitability of the location and refined analogues sites which included soil suitability for cumin cultivation (337 analogues sites out of 453) were identified. Rainfall trend analysis from the rainfall data of past 110 years indicated that analogues sites are showing the rainfall trend suited for cumin cultivation. This is the first report on the use of climate analogues tool to identify analogues sites with soil suitability and rainfall trend analysis of the identified analogues sites.
Messenger RNAs Mobile in Salix Matsudana Grafts Were in Association With Plant Rooting
Authors: Peng Yin , Xiao Liu , Baoliang Lan , Yu Cui , Yan Wang , Jichen Xu 
Published: June 2021

Abstract
Messenger RNAs exchanged between scions and rootstocks of grafted plants seriously affect their traits performance. The study goals were to identify the long-distance mRNA transmission events in grafted willows using a transcriptome analysis and to reveal the possible effects on rooting traits. The results showed that the Salix matsudana variety 9901 has better rooting ability than YJ, which reasonably improved the rooting performance of the heterologous grafts 9901 (scion) / YJ (rootstock). A transcriptome analysis showed that 2,948 differentially expressed genes (DEGs) were present in the rootstock of 9901/YJ grafted plants in comparison with YJ/YJ. Among them, 692 were identified as mRNAs moved from 9901 scion based on a SNP analysis of two parents. They were mostly 1,001–1,500 bp, had 40–45% GC contents, or had expression abundance values less than 10. However, mRNAs over 4,001 bp, having 50–55% GC contents, or having expression abundance values of 10–20 were preferentially transferred. Eight mRNAs subjected to long-distance trafficking were involved in the plant hormone pathways and may significantly promote the root growth of grafted plants. Thus, heterologous grafts of Salix matsudana could efficiently influence plant rooting since of the mRNAs transport from scion to rootstock. Thus, the grafting parents and grafting patterns would be much concerned in the breeding process to gain the expected results in future.
Burden of COVID-19 on Primary Care: a Prospective Nationwide Observational Study
Authors: Vaes Bert , Vos Bénédicte , Foidart Maxime , De Schreye Robrecht , Schrans Diego , Philips Hilde , Aertgeerts Bert , Doggen Kris 
Published: June 2021

Abstract
BackgroundThe COVID-19 outbreak had an important impact on general practice, e.g., the lack of personal protective equipment (PPE) and the switch to telephone consultations. We installed a monitoring instrument and reported the burden the COVID-19 pandemic placed on Belgian general practitioners (GPs) during the first wave of the pandemic.MethodsWe conducted an observational longitudinal prospective study from the 19th of March until the 17th of August 2020. Daily data were collected by using a structured electronic form integrated into the GPs’ electronic medical records (EMRs). Data were collected on the GPs’ need for support and workload, epidemiological indicators and the availability of PPE. Reports with graphical presentations were made for GP circles and primary care zones, and governments of different administrative levels had access to all data to guide their policy.ResultsA total of 3.769 different GP centres participated, which included more than 10.000 GPs. Throughout the first three weeks, 20% declared they had insufficient resources for the following week. Approximately 10% continued to report this during the entire study. The majority reported being able to complete their daily tasks without loss of quality. During the first week, 30% indicated an increased workload. Afterwards, this number decreased and stabilized to an average of 10-20%. More than 70% of the consultations in March 2020 were conducted by telephone. This percentage declined in April and stabilized at approximately 30% in June 2020. Consultations due to respiratory symptoms peaked at 4000/100,000 at the beginning of the outbreak, then decreased over time along with the COVID-19 incidence. We noticed a lack of disinfectant hand gel, surgical masks and FFP2 masks, the latter remaining problematic in the long term.ConclusionWe introduced an instrument in Belgian EMR systems to monitor the burden on GPs during the first wave of the COVID-19 pandemic. The lack of PPE and increased workload were considered to be the main obstacles. A large number of the GP offices switched to teleconsultations to provide healthcare. Our monitoring instrument provided information for policy makers to intervene on a local level.
First report of Encyrtid parasitiod, Copidosomyia ambiguous (Subba rao) Hymenoptera: Chalcidoidea: Encyrtidae) on Mallada desjardinsi (Navas) an indigenous predator of Aleurodicus rugioperculatus Martin in India
Authors: tnau Elango , A Aravind , Nelson Jeyarajan , M Ayyamperumal 
Published: June 2021

Abstract
Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae), commonly known as the rugose spiraling whitefly (RSW), is an invasive pest on coconut palm (Cocos nucifera L) in southern parts of India. To minimize crop losses, we currently employ a biological control agent, i.e., Mallada desjardinsi (Navas), which is an entomophagous predator for RSW. This voracious feeding predator belongs to the Chrysopidae family. However, under natural conditions, Mallada desjardinsi faces severe parasitoid attacks that hinder its multiplication and predating on RSW. In this study, we report the first incidence of Encyrtid parasitoid, Copidosomyia ambiguous (Subba Rao) (Hymenoptera: Chalcidoidea: Encyrtidae) on Mallada desjardinsi, in India.
ER-mitochondria contacts underline cannabinoid regulation of calcium signaling in astrocytes
Authors: Serrat Roman , Covelo Ana , Kouskoff Vladimir , Delcasso Sebastien , Ruiz-Calvo Andrea , Chenouard Nicolas , Stella Carol , Blancard Corinne , Salin Benedicte , Julio-Kalajzić Francisca , Cannich Astrid , Massa Federico , Varilh Marjorie , Deforges Severine , Robin Laurie , De Stefani Diego , Busquets-Garcia Arnau , Gambino Frederic , Beyeler Anna , Pouvreau Sandrine , Marsicano Giovanni 
Published: June 2021

Abstract
Intracellular calcium signaling underlies the astroglial control of brain functions. However, the cellular mechanisms regulating calcium handling by astrocytes are far from being understood. Mitochondria-endoplasmic reticulum contacts (MERCs) are key determinants of calcium dynamics, but their functional impact on astroglial regulation of brain information processing is currently unexplored. Here we show that the activation of astrocyte mitochondrial-associated CB1 receptors (mtCB1) regulates MERCs-dependent intracellular calcium signaling, thereby determining the synaptic functions of these cells. In vitro and in vivo stimulation of mtCB1 receptors promotes calcium transfer from the endoplasmic reticulum to mitochondria through a specific molecular cascade, involving AKT signaling, IPR3 receptors and different components of the mitochondrial calcium uniporter complex (MCU). Physiologically, mtCB1-dependent mitochondrial calcium uptake determines the dynamics of cytosolic calcium events in astrocytes upon endocannabinoid mobilization. Accordingly, electrophysiological recordings in hippocampal slices showed that astrocyte-specific mtCB1 receptors exclusion or dominant negative MCU expression blocks lateral synaptic potentiation, through which astrocytes integrate the activity of distant synapses. Altogether, these data reveal a cellular endocannabinoid link between astroglial MERCs and the regulation of brain network functions.
Statistical Elucidations of The Seasonal Ambience of Physicochemical Characteristics of Coastal Waters Around South Andaman, India
Authors: Shankar Venkatesan Shiva , Purti Neelam , Kesavan Dharanirajan , Mohan Pm , Subramanian Narayani , Kaviarasan Thanamegam , Gokul Murugaiah Santhosh , Gadi Padmavati , Ravindaran Satyakeerthy Thonduparambil 
Published: June 2021

Abstract
The coastal areas of Port Blair in Andaman are threatened by severe water pollution due to the human settlements in these regions. The objective of this study was to determine the level of pollution in ten different anthropogenically dynamic coastal regions by assessing the seasonal variations of various physicochemical characteristics. The regions selected for this study were Burmanallah (BA), Carbyn's Cove (CC), Chattam (CH), Flat Bay (FB), HADDO Harbour (HH), Junglighat Bay (JB), Minne Bay (MB), Phoenix Bay (PB), Sisostris Bay (SB) and Wandur (WA) in South Andaman. The study was carried out from January 2018 to December 2018 to investigate seasonal variations in the physicochemical parameters such as pH, temperature, salinity, dissolved oxygen, nitrite, nitrate, ammonia, silicate, phosphate, and chlorophyll-α using multivariate statistical analysis. Statistical analyses suggest that the regions of JB and MB were highly polluted while, BA, CC, FB, and WA were the least polluted. Landuse and land cover analysis of the study area further facilitated and supported the multivariate statistical results.
Circulating miRNA Profiles Associated with Lung Function Decline
Authors: Cheng Man , Xiao Yang , Ye Zi , Yu Linling , Wang Bin , Feng Xiaobin , Mu Ge , Ma Jixuan , Chen Weihong 
Published: June 2021

Abstract
Background: Lung function decline and miRNAs are known to involve in the pathogenesis process of respiratory diseases. However, the association between miRNA profiles and lung function decline was rarely elucidated. This study aimed to compare the expression pattern of plasma miRNAs among people with rapid lung function decline and healthy controls. Methods: Ten controls and 10 cases with rapid lung function decline in last 3 years were enrolled from the Wuhan-Zhuhai cohort. The miRNA sequencing and bioinformatics analysis were carried out to explore miRNAs expression and possible signal pathways. Pearson correlation analysis was carried out to assess the associations between lung function and miRNAs expression levels.Results: A total of 1209 detected miRNAs were differently expression in the reaseach participants. Among them, 17 miRNAs (miR-6749-5p, miR-6797-3p, miR-4468, miR-4301, miR-629-3p, miR-4713-3p, miR-486-3p, miR-450a-1-3p, miR-4732-5p, miR-514a-5p, miR-193b-5p, miR-6749-5p, miR-3168, miR-4691-5p, miR-6730-5p, miR-184, miR-486-5p) were significantly down-regulated in cases with reduced lung function. The expression levels of these miRNAs, except miR-514a-5p and miR-3168, were significantly correlated with lung function parameters. Through bioinformatics analysis, the Wnt signaling pathway, FoxO signaling pathway, MAPK signaling pathway, Pathway in cancer, inflammatory mediator regulation of TRP channels, and TGF-β signaling pathway maybe involved in mediating the association between 17 identified miRNAs and lung function decline.Conclusions: The plasma miRNA profile of people with rapid lung function decline are different when compared with healthy controls. These miRNAs might become research candidates or biomarkers in the early progression of respiratory diseases.
Benefits And Harms Of Implementing [18F]FDG-PET/CT For Diagnosing Recurrent Breast Cancer: A Prospective Clinical Study
Authors: Vogsen Marianne , Jensen Jeanette Dupont , Gerke Oke , Jylling Anne Marie Bak , Asmussen Jon Thor , Braad Poul-Erik , Thye-Rønn Peter , Søe Katrine Lydolph , Ewertz Marianne , Hildebrandt Malene Grubbe 
Published: June 2021

Abstract
Background [18F]-fluorodeoxyglucose-positron emission tomography / computed tomography ([18F]FDG-PET/CT) has been implemented sporadically in hospital settings as the standard of care examination for recurrent breast cancer. We aimed to explore the clinical impact of implementing [18F]FDG-PET/CT for patients with clinically suspected recurrent breast cancer and validate the diagnostic accuracy. Methods Women with suspected distant recurrent breast cancer were prospectively enrolled in the study between September 2017 and August 2019. [18F]FDG-PET/CT was performed, and the appearance of incidental benign and malignant findings was registered. Additional examinations, complications, and the final diagnosis were registered to reflect the clinical consequence of such findings. The diagnostic accuracy of [18F]FDG-PET/CT as a stand-alone examination was analyzed. Biopsy and follow-up were used as a reference standard. Results [18F]FDG-PET/CT reported breast cancer metastases in 72 of 225 women (32.0%), and metastases were verified by biopsy in 52 (52/225, 23.1%). Prior probability and posterior probability of a positive test for suspected metastatic cancer and incidental malignancies were 27%/85% and 4%/20%, respectively. Suspected malignant incidental findings were reported in 46 patients (46/225, 20.4%), leading to further examinations and final detection of nine synchronous cancers (9/225, 4.0%). These cancers originated from the lung, thyroid, skin, pancreas, peritoneum, breast, kidney, one was malignant melanoma, and one was hematological cancer. False-positive incidental malignant findings were examined in 37/225 patients (16.4%), mainly in the colon (n = 12) and thyroid gland (n = 12). Ten incidental findings suspicious for benign disease were suggested by [18F]FDG-PET/CT, and further examinations resulted in the detection of three benign conditions requiring treatment. Sensitivity, specificity, and AUC-ROC for diagnosing distant metastases were 1.00 (0.93-1.0), 0.88 (0.82-0.92), and 0.98 (95% CI 0.97-0.99), respectively.Conclusion [18F]FDG-PET/CT provided a high posterior probability of positive test, and a negative test was able to rule out distant metastases in women with clinically suspected recurrent breast cancer. One-fifth of patients examined for incidental findings detected on [18F]FDG-PET/CT were diagnosed with clinically relevant conditions. Further examinations of false-positive incidental findings in one of six women should be weighed against the high accuracy for diagnosing metastatic breast cancer.Trial registration: Clinical.Trials.gov. NCT03358589. Registered 30 November 2017 - Retrospectively registered, http://www.ClinicalTrials.gov
Development of a 3-microRNA signature and nomogram for predicting the survival of patients with uveal melanoma
Authors: Ye Hongqaun , Tang Jing , Lu Jianqun , Qi Wan 
Published: June 2021

Abstract
Background The aim of this study was to develop a robust miRNA signature and construct a nomogram model associated with uveal melanoma (UM) to improve prognosis prediction.Methods MiRNA and mRNA sequencing data of 80 uveal melanoma samples were downloaded from The Cancer Genome Atlas (TCGA) database. The patients were further randomly assigned to a training set (n = 40, used to identify key miRNAs) and a testing set (n = 40, used to internal verify the signature). Then miRNAs data of GSE84976 and GSE68828 were downloaded from Gene Expression Omniniub (GEO) database for outside verification. Combining univariate analysis and LASSO methods for identifying a robust miRNA biomarker in training set and the signature was validated in testing set and outside dataset. A prognostic nomogram was constructed and decision curve analyses and reduction curve analyses were performed to evaluate the clinical usefulness of the nomogram. A miRNA-mRNA network in UM was constructed and pathway enrichment of these miRNAs was conducted based on the genes in network.Results In total, a 3-miRNA was identified and validated which can robustly predict UM patient’s survival. According to univariate and multivariate analyses, age at diagnosis, tumor node metastasis (TNM) classification, stage and 3-miRNA signature significantly correlated with the survival outcomes. These characteristics were used to establish nomogram. The nomogram showed good accuracies in predicting 1 and 3 years overall survival and the decision curve revealed the clinical usefulness of our nomogram. What’s more, a miRNA-mRNA network was constructed. Pathway enrichment showed that this network was largely take part in mRNA processing, mRNA surveillance pathway, spliceosome and so no.Conclusions We developed a 3-miRNA biomarker and constructed a prognostic nomogram, which afforded a quantitative tool for predicting the survival of UM. Our finding also provided some new potential targets for the treatment of UM.
Long Noncoding RNA SNHG4 Remits Lipopolysaccharide‐Engendered Inflammatory Lung Damage by Inhibiting METTL3 - Mediated m6A Level of STAT2 mRNA
Authors: Li Si-Xiu , Yan Wen , Liu Jian-Ping , Zhao Yu-Juan , Chen Lu 
Published: June 2021

Abstract

Background: Emerging evidence suggests that long non coding RNA (lncRNA) small nucleolar RNA host gene 4 (SNHG4) has become a new insight into lipopolysaccharide (LPS) - induced microglia inflammation, its role in neonatal pneumonia (NP) remains to be largely unrevealed.Methods: RT-qPCR was used to determine SNHG4 and METTL3 expression in the serum from NP patients and normal volunteers, as well as in WI-38 cells treated with LPS. The SNHG4 overexpression vector (pcDNA-SNHG4) was transfected into LPS - treated cells. CCK-8, Transwell, annexin V-FITC/PI and ELISA assays were used to determine cell proliferation, migration, apoptosis and contents of IL-6, TNF-α, SOD and MDA, respectively. The level of SNHG4 in the promoter region of METTL3 was assessed with RIP assay. m6A quantitative analysis illustrated the m6A level with or without SNHG4 overexpression or METTL3 silencing. Bioinformatics analysis and RIP-PCR were used to predict and validate YTHDF1 - mediated m6A levels on signal transducer and activator of transcription 2 (STAT2) mRNA in METTL3 inhibited cells. Then rescue experiments were performed to explore effects of SNHG4 and METTL3 or STAT2 on LPS-treated cell functions. Subsequently, in vivo functional experiments were performed to investigate the role of SNHG4 in LPS induced pneumonia in mice. Results: SNHG4 was downregulated and METTL3 was upregulated in NP patients and LPS-treated cells. SNHG4 overexpression facilitated cell proliferation, migration and SOD concentration, and inhibited apoptosis and IL-6, TNF-α and MDA contents. Mechanistically, SNHG4 bound with METTL3 and downregulated METTL3 expression. Besides, total m6A modification level was lower in the SNHG4 overexpressed or METTL3 inhibited cells. METTL3 interference reduced m6A levels of STAT2 mRNA, decreased STAT2 mRNA stability and promoted STAT2 translation level. METTL3 or STAT2 upregulated reversed the effects of SNHG4 overexpression on LPS - treated cell functions. Conclusions: This study reveals that SNHG4 promotes LPS induced inflammation in human lung fibroblasts and mouse lung tissues in vitro and in vivo by inhibiting METTL3 - mediated m6A level of STAT2 mRNA, which may provide a potential therapeutic mechanism for NP.