bluuebunny/crossref_metadata_2025_split huggingface dataset:
YAP/TAZ: Key Players for Rheumatoid Arthritis Severity by Driving Fibroblast Like Synoviocytes Phenotype and Fibro-Inflammatory Response
Authors: Caire Robin , Audoux Estelle , Courbon Guillaume , Michaud Eva , Petit Claudie , Dalix Elisa , Chafchafi Marwa , Thomas Mireille , Vanden-Bossche Arnaud , Navarro Laurent , Linossier Marie-Thérèse , Peyroche Sylvie , Guignandon Alain , Vico Laurence , Paul Stephane , Marotte Hubert 
Published: December 2021

ObjectiveThe role of YAP/TAZ, two transcriptional co-activators involved in several cancers, was investigated in rheumatoid arthritis (RA).MethodsFibroblast like synoviocytes (FLS) from patients with RA or osteoarthritis were cultured in 2D or into 3D synovial organoids. Arthritis rat model (n=28) and colitis mouse model (n=21) were used. YAP/TAZ transcriptional activity was inhibited by verteporfin (VP). Multiple techniques were used to assess gene and/or protein expression and/or localization, cell phenotype (invasion, proliferation, apoptosis), bone erosion, and synovial stiffness.ResultsYAP/TAZ were transcriptionally active in arthritis (19-fold increase for CTGF expression, a YAP target gene, in RA vs. OA organoids; p&lt;0.05). Stiff support of culture or pro-inflammatory cytokines further enhanced YAP/TAZ transcriptional activity in RA FLS. Inhibiting YAP/TAZ transcriptional activity with VP restored a common phenotype in RA FLS with a decrease in apoptosis resistance, proliferation, invasion, and inflammatory response. Consequently, VP blunted hyperplasic lining layer formation in RA synovial organoids. In vivo, VP treatment strongly reduced arthritis severity (mean arthritic index at 3.1 in arthritic group vs. 2.0 in VP treated group; p&lt;0.01) by restoring synovial homeostasis and decreasing systemic inflammation. YAP/TAZ transcriptional activity also enhanced synovial membrane stiffening in vivo, thus creating a vicious loop with the maintenance of YAP/TAZ activation over time in FLS. YAP/TAZ inhibition was also effective in another inflammatory model of mouse colitis.ConclusionOur work reveals that YAP/TAZ were critical factors during arthritis. Thus, their transcriptional inhibition could be relevant to treat inflammatory related diseases.
The Occurrence of Seizure Clusters in Patients With Epilepsy Is Partly Determined by Epilepsy Severity: A Single-Center Retrospective Observational Study
Authors: Zhong Rui , Chen Qingling , Zhang Xinyue , Lin Weihong 
Published: December 2021

Purpose: This retrospective observational study aimed to investigate the self-reported prevalence of seizure clusters (SCs) in patients with epilepsy (PWE) and its relationship with clinical characteristics.Methods: We retrospectively analyzed data from consecutive PWE from our hospital in northeastern China. Data were collected from the databank of a tertiary epilepsy center. Logistic regression models were employed to investigate the relationships between the individual patient demographic/clinical variables and the occurrence of SC.Results: In total, 606 consecutive PWE were included in the final analysis, and 268 (44.2%) patients experienced at least one seizure cluster. In multivariate logistic regression models, age (OR: 1.014; 95% CI: 1.002–1.027; p = 0.02), seizure frequency (OR: 2.08; 95% CI: 1.555–2.783; p &lt; 0.001), multiple seizure types (OR: 5.111; 95% CI: 1.737–15.043; p = 0.003), number of current anti-seizure medications (ASM) (OR: 1.533; 95% CI: 1.15–2.042; p = 0.004), drug-resistant epilepsy (OR: 1.987; 95% CI: 1.159–3.407; p = 0.013), and a history of status epilepticus (OR: 1.903; 95% CI: 1.24–2.922; p = 0.003) were independent variables associated with a history of SC in PWE.Conclusion: Seizure clusters (SCs) are common occurrences at our study center. The occurrence of SC in individuals with epilepsy, to some extent, is determined by the epilepsy severity.
Relationship Between Lipodystrophy, Body Composition, Metabolic Profile, and Serum Levels of Adipocytokines
Authors: Sacilotto Lívia Bertazzo , Papini Silvia Justina , Mendes Adriana Lucia , Gatto Mariana , Pereira Paulo Câmara Marques , Corrente José Eduardo , Silva Julhiany de Fátima da 
Published: December 2021

Background: Despite the benefits in improving the clinical state of people living with HIV/aids (PLWHA), some side effects associated with the use of antiretroviral therapy (ART) are reported. Redistribution of body fat has been associated with treatment and is characterized by morphological changes, also known as lipodystrophy. The complications of metabolic and morphological changes in these individuals seem to increase the risk of cardiovascular disease. Adipocytokines are proteins that have essential functions in biological processes, in which the levels of these proteins are related to the pathogenesis of metabolic syndrome (MS) and cardiovascular disease. Recent studies have shown that such levels are generally modified in PLWHA, regardless of whether the treatment is established or not. An application of methods for body fat estimation in patients with fat redistribution, as in the case of aids, especially those that quantify body fat by segments, appears to clarify these alterations and plays an important role in the development of multiprofessional treatment.Objectives: This investigation was carried out to compare and correlate body composition, biochemical metabolic parameters, and levels of adipocytokines and cytokines of PLWHA, with and without lipodystrophy, with individuals with negative HIV serology and stratified by sex.Material and Methods: This is a cross-sectional study in which body composition, metabolic and anthropometric changes, and levels of adipocytokines of 110 individuals were assessed. These individuals were paired in sex, age, and body mass index (BMI) and subdivided into three groups: PLWHA with and without a clinical diagnosis of lipodystrophy associated with HIV, and a group control.Results: Collinearity was identified both in the general sample and for genders of the waist-to-height ratio (WHtR) with all anthropometric parameters, except for muscle mass. The results show strong association between IFN-γ and TNF-α both in the general sample and for genders and moderate correlation between leptin and fasting glucose for women; worsening of the triglyceride profile in both women with lipodystrophy compared with the control group and men without lipodystrophy compared with the control group; higher serum TNF-α values among men without lipodystrophy compared to those with HIV-associated lipodystrophy (HALS).Conclusions: The results of this study underline that, considering the manifestations of the syndrome, these patients have a high-risk endocrine metabolic profile for cardiovascular events.
Clinical Significance and Prognostic Value of the Maximum Standardized Uptake Value of 18F-Flurodeoxyglucose Positron Emission Tomography–Computed Tomography in Colorectal Cancer
Authors: Yin Yi-xin , Xie Ming-zhi , Liang Xin-qiang , Ye Meng-ling , Li Ji-lin , Hu Bang-li 
Published: December 2021

BackgroundThe role of 18F-flurodeoxyglucose (18F-FDG) positron emission tomography–computed tomography (PET/CT) in colorectal cancer (CRC) remains unclear. This study aimed to explore the association of the maximum standardized uptake value (SUVmax), a parameter of 18F-FDG PET/CT, with KRAS mutation, the Ki-67 index, and survival in patients with CRC.MethodsData of 66 patients with CRC who underwent 18F-FDG PET/CT was retrospectively collected in our center. The clinical significance of the SUVmax in CRC and the association of the SUVmax with KRAS mutation and the Ki-67 index were determined. A meta-analysis was conducted by a systematic search of PubMed, Web of Science, and CNKI databases, and the data from published articles were combined with that of our study. The association of the SUVmax with KRAS mutation and the Ki-67 index was determined using the odds ratio to estimate the pooled results. The hazard ratio was used to quantitatively evaluate the prognosis of the SUVmax in CRC.ResultsBy analyzing the data of 66 patients with CRC, the SUVmax was found not to be related to the tumor-node-metastasis stage, clinical stage, sex, and KRAS mutation but was related to the tumor location and nerve invasion. The SUVmax had no significant correlation with the tumor biomarkers and the Ki-67 index. Data of 17 studies indicated that the SUVmax was significantly increased in the mutated type compared with the wild type of KRAS in CRC; four studies showed that there was no remarkable difference between patients with a high and low Ki-67 index score regarding the SUVmax. Twelve studies revealed that the SUVmax had no significant association with overall survival and disease-free survival in CRC patients.ConclusionsBased on the combined data, this study demonstrated that the SUVmax of 18F-FDG PET/CT was different between colon and rectal cancers and associated with KRAS mutation but not the Ki-67 index; there was no significant association between the SUVmax and survival of patients with CRC.
In Search of Autonomy: Dancing With Rules
Authors: Heldal Frode , Dehlin Erlend 
Published: December 2021

Purpose:Autonomy in organisations cannot exist without rules nor relationships. Yet, previous research tends to elicit understandings of autonomy as freedom from external constraints to enact free individual will. And there are numerous positive effects related to autonomy at work. But research has not kept pace with modern-day organisations that are highly flexible and dynamic. Current understandings of autonomy are static. Autonomy is mainly regarded as something individuals possess, more or less constricted by rules. Our purpose is to contribute a more flexible and practice-oriented concept of autonomy to answer the research question: How is autonomy developed and practiced in relation to formal rules in high-risk organisations?Design:To investigate autonomy as a dynamic and flexible concept, we draw on two case studies comprised of a total of 52 interviews and more than 10 h of observation. The cases include a factory and a hospital unit.Findings:We suggest, based on the data, that autonomy is a relational phenomenon. We suggest four different autonomy-rule dynamics: Passive, loyal, self-promoting, and co-generative learning.Research Implications:Regarding autonomy as relational rather than individual contributes to our understanding of organisations as always in the making. In this, we emphasise the interactive element of autonomy.Practical Implications:Practitioners and managers may use our suggestions to work with autonomy in a different way, spurring creativity and improvisation by constructively using rules.Originality:Little research has paid attention to the concept of autonomy (despite its importance), and arguably, a trend in the available research concerns a commodification of the phenomenon, primarily aligning autonomy with (degrees of) negative freedom and individual decision making. We unpack the concept with attention to interaction – what we have called dancing with rules.
“Who I Am Now, Is More Me.” An Interview Study of Patients’ Reflections 10 Years After Exhaustion Disorder
Authors: Ellbin Susanne , Jonsdottir Ingibjörg H. , Bååthe Fredrik 
Published: December 2021

Aim: To achieve a deeper understanding of the patient’s perception regarding individual aspects related to the development of exhaustion, hindering and supporting factors in the recovery process, and potential remaining consequences, 7–12 years after receiving an exhaustion disorder diagnosis.Participants and Methods: Twenty patients previously diagnosed with and treated for exhaustion disorder were interviewed 7–12 years after onset of the disease. The semi-structured interviews were transcribed verbatim and analyzed with inductive content analysis.Results: Three main themes with patterns of shared meaning resulted from the analysis: “it’s about who I am,” “becoming a more authentic me,” and “the struggle never ends.” The interviewees described rehabilitation from exhaustion disorder as the start of an important personal development toward a truer and more authentic self-image. They perceived this as an ongoing long-lasting process where learned behavior and thought patterns related to overcommitment and overcompliance needed to be re-evaluated. The results also convey long-term consequences such as cognitive difficulties and reduces energy, uncertainty about one’s own health, and the need to prioritize among one’s relationships.Conclusion: Patients with exhaustion disorder are still struggling with dysfunctional strategies and functional impairments such as cognitive problems which limit their lives, 10 years after receiving their exhaustion disorder diagnosis. While informants describe some positive consequences of ED, the results also emphasize the importance of acknowledging that the patients are embedded in systems of relationships, in working life as well as in family life. This needs to be considered, together with other aspects, when working toward prevention of stress-related mental health problems.
Sleep Disturbances Are Associated With Depressive Symptoms in a Chinese Population: The Rugao Longevity and Aging Cohort
Authors: Qiao Chunhong , Zhang Hui , Song Qi , Wang Xi , Wang Xiaofeng , Yao Yin 
Published: December 2021

Objective: To investigate the cross-sectional and longitudinal relationships between sleep disturbances and depressive symptoms in older Chinese adults.Methods: This study included baseline and 3.5-year follow-up data of 1,631 Chinese men and women aged 70 years or older from the aging arm of the Rugao Longevity and Aging Study. Depressive symptoms were assessed by the 15-item Geriatric Depression Scale (GDS). Sleep disturbances were assessed by using the Pittsburgh Sleep Quality Index (PSQI). Logistic regression models were used to estimate the odds ratios (ORs) of the associations.Results: In the cross-sectional analysis, individuals with greater total PSQI scores exhibited significantly higher risk of “depressive symptoms” (OR: 1.31, 95% CI: 1.21–1.41) and “some depressive symptoms” (OR: 1.22, 95% CI: 1.17–1.28). Specifically, higher scores on the sleep efficiency PSQI subscale were associated with greater odds for “depressive symptoms” (OR: 1.54, 95% CI: 1.30–1.84) and “some depressive symptoms” (OR: 1.42, 95% CI: 1.29–1.57). Our longitudinal analyses indicated an association between greater PSQI total scores at baseline and greater odds of having “some depressive symptoms” at follow-up (OR: 1.07, 95% CI: 1.00–1.14). Additionally, higher scores on the sleep efficiency PSQI subscale had an association with higher odds for “some depressive symptoms” (OR: 1.21, 95% CI: 1.04–1.41).Conclusions: Poor self-reported global sleep quality and sleep efficiency PSQI subscale scores were associated with levels of depressive symptoms in an older Chinese population, indicating that global sleep quality and sleep efficiency may be risk factors for depression and can possibly predict the levels of depressive symptoms.
Future Trends in Disability and Its Determinants Among Chinese Community Patients With Anxiety Disorders: Evidence From a 5-Year Follow-Up Study
Authors: Liu Zhaorui , Li Peijun , Yin Huifang , Li Minghui , Yan Jie , Ma Chao , Ding Hua , Li Qiang , Huang Zhengjing , Yan Yongping , Kou Changgui , Hu Mi , Wen Jing , Chen Shulin , Jia Cunxian , Huang Yueqin , Xu Guangming 
Published: December 2021

Background: Anxiety disorders (ADs) are a group of disorders with a high disability rate and bring a huge social burden. In China, information on future trends in the disability among community ADs patients and its determinants are rare. The objectives of this study are to describe the future trends in the disability among ADs patients living in community and to investigate the determinants of the disability.Methods: Participants diagnosed with 12-month ADs in the China Mental Health Survey (CMHS) were followed up by telephone from April to June 2018 to assess the future trends in the disability in a 5-year interval using the World Health Organization's Disability Assessment Schedule 2.0. The disability rate was reported and its determinants were analyzed by complex sample design multivariate logistic regression.Results: Totally 271 patients were interviewed by telephone and 33 informants finished proxy interviews. The disability rates were 45.9% and 14.3% among ADs patients at baseline and during the follow-up. Patients with general anxiety disorder (GAD) or agoraphobia with/without panic disorder (AGP) had the lower decrease and higher disability during the follow-up than patients with other subtypes. Patients aged in middle age (aged 40–49 years old, OR = 11.12, 95% CI: 4.16–29.72), having disability at baseline (OR = 7.18, 95% CI: 1.37–37.73), having comorbidity with three or more physical diseases (OR = 9.27, 95% CI: 2.48–34.71), and having comorbidity with other mental disorders (OR = 3.97, 95% CI: 1.13–13.96) had higher disability during the follow-up.Conclusions: The disability rate tends to decrease among ADs patients living in communities. Treatment priority should be given for ADs patients with disability and those in middle age. Treatments for the comorbidity of other mental disorders or physical diseases should be considered when treating anxiety.
Efecto del pH y la salinidad en el crecimiento y luminiscencia de una bacteria marina como biosensor ambiental
Authors: Pérez Lemes Vener , Delgado Gómez Yolaine 
Published: December 2021

Effect of pH and salinity on the growth and luminescence of a marine bacterium as an environmental biosensor
Resumen
El trabajo se desarrolló en el Centro de Estudios de Proteínas de la Facultad de Biología de la Universidad de La Habana en septiembre de 2012 con el objetivo de evaluar el crecimiento y la luminiscencia de la cepa bacteriana CBM-784 en el medio LM a diferentes intervalos de pH y salinidad y determinar los rangos óptimos para sus crecimiento y luminiscencia. Los resultados demuestran que la bacteria luminiscente CBM-784 mostró su mejor crecimiento y luminiscencia a pH= 8,3 y concentraciones de NaCl entre 2,95 y 3,5%.
Palabras clave: bacterias; ecología marina; luciferase; luminiscencia bacteriana; medio de cultivo.
Abstract
The work was developed at the Center for Protein Studies of the Faculty of Biology of the University of Havana in September 2012 with the objective of evaluating the growth and luminescence of the bacterial strain CBM-784 in the LM medium at different intervals of pH and salinity and to determine the optimal ranges for its growth and luminescence. The results demonstrate that the luminescent bacterium CBM-784 showed its best growth and luminescence at pH = 8.3 and NaCl concentrations between 2.95 and 3.5%.
Keywords: bacteria; marine ecology; luciferase; bacterial luminescence; culture medium.
Cuantificación de la recarga de agua en el sistema de riego Catarama mediante Modelo Renata
Authors: Jiménez Valencia Govanny , Félix Mera John Enrique , Alarcón Loor Jose Ramón 
Published: December 2021

Cuantification of Catarama irrigation system recharge by Renata Model
Resumen
El objetivo de la investigación es cuantificar la recarga de agua generada en el sistema de riego Catarama (Cuenca del río Catarama) perteneciente a la provincia de Los Ríos (Ecuador), mediante la aplicación del modelo numérico RENATA (Recarga NATural a los Acuíferos) desarrollado por el Instituto Geológico y Minero de España (IGME) y la Diputación de Alicante. En el estudio se calculan los volúmenes de agua que son generados por la infiltración ocasionada por la lluvia y por el retorno de riego proveniente de las dotaciones de agua asignadas a los diferentes cultivos existentes en la zona. Para la determinación de la recarga se consideraron diferentes variables climáticas influyentes como precipitación, temperatura, evapotranspiración potencial y real; así como las diferentes características de los suelos del sector. A partir de estas variables RENATA pudo generar los resultados de la modelación en el sistema de riego, en base a los datos obtenidos de series climáticas mensuales correspondientes al periodo enero 2005 - diciembre 2014, obteniendo durante este tiempo una recarga teórica de 57,139 Hm3 en un área total aproximada de 6047 ha.
Palabras clave: RENATA; variable; precipitación; recarga; infiltración; riego.
Abstract
In this article, the water recharge in the Catarama irrigation system (Catarama river basin), Los Ríos, Ecuador is quantified through the application of the RENATA numerical model (Natural Aquifer recharge) developed by the Instituto Geológico y Minero de España (IGME) and Diputación de Alicante. The study calculates the volumes of water generated by the infiltration caused by rainfull and the return of irrigation from the water assigned to the different crops in the area. To determine the recharge, different influential climatic variables were considered: precipitation, temperature, potential and the current evapotranspiration; as well as the different characteristics of the sector’s soils. From these variables, RENATA was able to generate the results of the irrigation system modeling, based on the data obtained from monthly climatic series corresponding to the period January 2005 - December 2014, getting during this time a theoretical recharge of 57,139 Hm3, in an approximate total area of 6047 ha.
Keywords: RENATA; Variable; Precipitation; Recharge; Infiltration; Irrigation.