bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors and Predictive Value of Perioperative Neurocognitive Disorders in Elderly Patients With Gastrointestinal Tumors
Authors: Li Yongli , Huang Hui-Fan , Le Yuan 
Published: March 2021

Abstract
Background: This study aims to investigate the risk factors of perioperative neurocognitive disorders (PNDs) mainly including postoperative cognitive dysfunction (POCD) in elderly patients with gastrointestinal tumors, and evaluate its predictive value.Methods: A total of 222 eligible elderly patients (≥65 years) scheduled for elective gastroenterectomy under general anesthesia were enrolled. The cognitive function assessment was carried out 1 day before surgery and 7 days after surgery. Receiver operating characteristic curve analysis was performed to evaluate the predictive value of risk factors for early POCD. The risk factors for POCD were analyzed using a multivariate logistic regression mode.Results: Of all the 222 enrolled patients, 91 (41.0%) developed early POCD and 40 (18.0%) were identified as major POCD within 7 days after the surgery. Visual analogue score (VAS, 1st day, resting) ≥4 (OR=7.618[3.231–17.962], P<0.001) and alcohol exposure (OR= 2.398[1.174–4.900], P=0.016) were independent risk factors for early POCD. VAS score (1st, resting) ≥4 (OR=13.823[4.779–39.981], P<0.001), preoperative white blood cell (WBC) levels ≥10x10*9/L(OR=5.548[1.128-26.221], P=0.035), blood loss ≥500ml (OR=3.317[1.094-10.059], P=0.034), history of hypertension (OR=3.046[1.267-7.322], P=0.013), and neutrophil–lymphocyte ratio (NLR) ≥2 (OR=3.261[1.020-10.419], P=0.046) were independent risk factors for major POCD. Receiver operating characteristic curve analysis indicated that VAS score (1st day, resting) was a significant predictor for major POCD with a cut-off value of 2.68 and an area under the curve of 0.860 (95% confidence interval: 0.801–0.920, P<0.001).Conclusions: The risk factors for early POCD after gastroenterectomy included high VAS score (1st day, resting) and alcohol exposure. High VAS score, preoperative WBC levels ≥10x10*9/L, blood loss ≥500ml, NLR ≥2, and history of hypertension were independent risk factors for major POCD. Among them, VAS score had a high predictive value.
Genotoxic and Mutagenic&nbsp;Effects of the Antifouling Biocide Chlorothalonil on the Estuarine Fish Micropogonias Furnieri, Desmarest, 1823
Authors: Castro Muryllo Santos , Penha Larissa Cristine Carvalho , Torres Thamires Alexsandra , Jorge Marianna Basso , Carvalho-Costa Luis Fernando , Fillmann Gilberto , Luvizotto-Santos Ricardo 
Published: March 2021

Abstract
Chlorothalonil is a widely used fungicide in agriculture, and as biocide in antifouling paints. Although it causes toxic effects on non-target organisms and can bioaccumulate in fish tissues, little is known about its sublethal effects. Thus, we evaluated the genotoxic and mutagenic effects of chlorothalonil in Micropogonias furnieri, an estuarine and commercially important fish with potential as a test organism for ecotoxicology assays. We showed that chlorothalonil exerts genotoxic (DNA damage) and mutagenic (micronuclei and nuclear abnormalities) in a dose-dependent manner (0.35 and 3.5 μg g-1). As the genomic instability may lead to carcinogenesis, our data can assist decision-makers with evidence for banning this compound since any benefit to portuary activities and maritime navigation is outweighed by the cost to aquatic ecosystems and to human health.
Clinico-Hematological and cytogenetic spectrum of adult myelodysplastic syndrome The first retrospective cross-sectional study in Iranian patients
Authors: Paridar Mostafa , Zibara Kazem , Ahmadi Seyed Esmaeil , Khosravi Abbas , Soleymani Maral , Azizi Ebrahim , Ghalesardi Omid Kiani 
Published: March 2021

Abstract
Background

Myelodysplastic syndrome (MDS), a heterogeneous group of hematopoietic malignancy, has been shown to present different cytogenetic abnormalities, risk factors, and clinico-hematological features in different populations and geographic areas. Herein, we determined the cytogenetic spectrum and clinico-hematological features of Iranian MDS patients for the first time.
Methods

This prospective cross-sectional study was conducted on 103 patients with MDS in Ahvaz, southwest of Iran, from 2014 to 2018. Clinical presentations, complete blood counts (CBC), and bone marrow (BM) biopsy samples were assessed. Perls' staining was used to evaluate BM iron storage. The cytogenetic evaluation was performed using the conventional G banding method on the BM.
Results

Patients’ median age was 62.3 (ranged from 50–76), and the majority were male (72.8%). The most common clinical symptom at the time of admission was fatigue (n = 33) followed by pallor (n = 27). The most common subgroup was MDS-Multi Lineage Dysplasia (MDS-MLD) (n = 38, 36.8%), followed by MDS-Single Lineage Dysplasia (MDS-SLD) (n = 28, 18.4%). A normal karyotype was observed in 59 patients (57.3%), while 44 patients (42.7%) had cytogenetic abnormalities. Trisomy 8 (+ 8) was the most common cytogenetic abnormality (n = 14) followed by dell 17p (n = 9) and monosomy 7 (-7) (n = 7). Twelve patients (11.65 %) were transformed to AML.
Conclusion

Our data betokened that among our MDS patients, Trisomy 8 is the predominant cytogenetic abnormality, and MDS-MLD and MDS-SLD are the most common of subtypes. Noteworthy, the male: female ratio was slightly higher in Iran than in previous reports from other parts of the world. Our study is the first report of the clinical, hematological, and cytogenetic spectrum of MDS patients in Iran
Modification of the Classical Percutaneous Extraperitoneal Procedure for Pediatric Inguinal Hernia to Reduce Complications: A Multiple-Centered Comparison Study
Authors: Luo Zhi-bin , Tu Jing-rong , Shi Hong-guang , Du Zhi-yong , Xiang Xian-cai , Chi Shui-qing , Yang De-hua , Li Kang , Li Shuai , Tang Shao-tao 
Published: March 2021

Abstract
Single-site laparoscopic percutaneous extraperitoneal ligation (SLPEL) for pediatric inguinal hernia gained popularity all over the world. However, complications associated with extraperitoneal knotting were not rare in the classical SLPEL(C-SLPEL) procedure. In order to overcome disadvantages, we herein developed the modified SLPEL (M-SLPEL) procedure, intact circuit ligation of the peritoneum around the internal ring using a homemade hernia needle with a single abdominal wall centesis. To evaluated the effectiveness of the M-SLPEL procedure to decrease adverse events associated with ligation knotting, a comparative study was carried out. A total of 3219 patients from multiple centers were divided into two groups according to the operative procedures: M-SLPEL group and C-SLPEL group. All patients were followed up. Data describing the clinical characteristics, operative time, postoperative hospital stay, and complications was collected and retrospectively analyzed. With equivalent operative time, postoperative hospital stay, there was statistically significant difference between two groups in terms of the overall complications incidence (2.6% in C-SLPEL Vs 0.11% in M-SLPEL, P=0.03), including pain in inguinal area, knot foreign body reaction, palpable knot, recurrence. Together, these findings suggest that the M-SLPEL procedure is an effective and safe approach, with unique advantage in reducing adverse events in the inguinal region.
Re-sequencing and transcriptomic analysis reveals rich DNA sequence variation and differential gene expression between varieties of Ziziphus jujuba
Authors: Li Na , Song Yuqin , Li Jie , Hao Ruijie , Feng Xinxin , Li Liulin 
Published: March 2021

Abstract
Background: Jujube is one of the characteristic fruit tree species in China. ‘Linhuang No. 1’, a cracking-resistant cultivar, and ‘Muzao’, a cracking-susceptible cultivar, were selected as materials by previous study. Whole-genome re-sequencing and transcriptome of ‘Linhuang No. 1’ and ‘Muzao’ allow the screening out of differently expressed genes with different gene structures between them. It could be helpful in explaining divergence/similarity of cracking resistance between the two cultivars.
Results: There are 664,129 mutation sites between ‘Linhuang No. 1’ and ‘Muzao’ by re-sequencing. To determine the genetic relationship of ‘Linhuang 1’, ‘Muzao’ and reference genome ‘Dongzao’, the characteristic mutation sites were analyzed by principal component analysis. The genetic relationship between ‘Linhuang No. 1’ and ‘Muzao’ was closer than that with ‘Dongzao’. A total of 431 differentially expressed genes was screened by transcriptomics, and 19 differentially expressed genes were screened by combining the transcriptomics with re-sequencing analysis. LOC107427052 (encoding nitrite reductase) was determined by KEGG enrichment analysis for further study.
Conclusions: The large base insertion was not in the domain region of the LOC107427052 gene CDS region. As verified by the finding that the base insertion did not affect protein translation. Our study has laid a foundation for the analysis of genetic information and the comparative nitrite metabolism of ‘Linhuang No. 1’ and ‘Muzao’.
Developmental Morphological Analyses on the Preglottal Salivary Gland in Japanese Quails (Coturnix Japonica)
Authors: Khalifa Mahmoud Osman , Abd-ElKareem Mahmoud , Gaber Wafaa , Li TS , Saleh Abdelmohaimen M. 
Published: March 2021

Abstract
To understand the development of the mucous preglottal salivary gland in Coturnix japonica (Japanese quail), morphological and histochemical studies were performed on 20 healthy Japanese quail embryos (aging from 10 th to 17 th incubation days) and 25 healthy quail chicks (aging from 0 th to 60 th days). The primordia of preglottal salivary gland was observed as an epithelial bud at the early embryonic stage, which then elongated and differentiated into secretory units by the end of this stage. In Japanese quails, the preglottal salivary gland was a mucous polystomatic tubulo-alveolar unpaired gland composed of two lateral portions and a middle one embedded into submucosa of the lingual root. The gland openings accompanied taste pore (8.17 μm) of taste buds associated salivary glands type; some skeletal muscle fibers embedded among secretory lobules extended from muscle cricohyoideus at 14 th day old quail chick. Also, both herbts corpuscles and secretory motor plexus could be detected among secretory lobules. Based on our investigations, the development of preglottal salivary gland could clearly be distinguished in the embryonic stage into prebud and bud stages at 10 th day old, cord and branching stages ended by cavitation at 11 th day old, canalization stage at 13 th day old, lobulation and secretory stages by the 17 th day old. This mucous secretion showed different histochemical reactions ended with highly alcinophilic mucous indicated highly sialomucin (acidic) content. Myoepithelial cells could be demonstrated at 17-day old quail embryo and there after, surrounded the secretory endpieces of the preglottal salivary gland.
Management of Chronic Knee Pain Caused by Postsurgical or Post-traumatic Neuroma of the Infrapatellar Branch of the Saphenous Nerve.
Authors: Regev Gilad J. , Shabat Dvir Ben , Khashan Morsi , Ofir Dror , Salame Khalil , Shapira Yuval , Kedem Ron , Lidar Zvi , Rochkind Shimon 
Published: March 2021

Abstract
Purpose: Injury to the infrapatellar branch of the saphenous nerve (IBSN) is a relatively common complication after knee surgery that can interfere with patient satisfaction and functional outcome. In some cases, trauma to this nerve can lead to the formation of a painful neuroma. The purpose of this study is to report the results of surgical treatment in a series of patients with IBSN painful neuroma.Methods: A retrospective review was made of 37 patients undergoing resection of IBSN neuroma at our institution, after failure of non-operative treatment for a minimum of 6 months.Results: Following surgery, 80% of the patients experienced statistically and clinically significant improvement in pain levels. The average NRS Pain score improved from 9.43±1.34 to 5.12±3.33 (p<0.001). The EQ-5D functional score improved from 10.48±2.33 to 7.84±2.19. Overall patient reported satisfaction from the surgical results was good to excellent in 72% of patients. Non-favorable prognosis was found to correlate with older age, multiple prior orthopedic surgeries at the knee area and failed prior attempts to resect an IBSN neuroma. Conclusion: We conclude that in patients suffering from IBSN painful neuroma, surgical resection results in a favorable prognosis.
Evaluation of optical coherence tomography angiography parameters in patients treated with hydroxychloroquine
Authors: Akhlaghi Mohammad Reza , Kianersi Farzan , Radmehr Hamed , Dehghani Alireza , Beni Afsaneh Naderi , Noorshargh Pegah 
Published: March 2021

Abstract
Background One of the major side effects of hydroxychloroquine is retinopathy. The aim of this study was to evaluate the Optical coherence tomography angiography (OCTA) parameters in a group of patients who have hydroxychloroquine-induced retinopathy based on Multifocal electroretinography (mfERG) with a group who do not have retinopathy.Method This is a Cross-Sectional Study. In this study, patients with RA or SLE who had been taking HCQ for at least 7 years were included. MfERG and OCTA imaging were performed for all patients. Patients were divided into Normal mfERG and Abnormal mfERG groups based on mfERG results. OCTA parameters were studied in these two groups.Result 61 patients (61 eyes) were included. 41 patients had SLE and 20 patients had RA. 40 patients (66.7%) had Abnormal mfERG. The mean VD in SCP layer was not significantly different between Normal mfERG and Abnormal mfERG groups (P-Value> 0.05). Mean VD in SCP layer was not significantly different between Normal mfERG and Abnormal mfERG groups (P-Value> 0.05). In RA subgroup, mean VD in SCP layer in PeriFovea region in Abnormal mfERG group was significantly lower than normal group (P-Value <0.05). Mean VD in DCP layer in Whole Image, Superior Hemi, Inferior Hemi, PeriFovea area in Abnormal mfERG group was significantly lower than normal group (P-Value <0.05). This discrepancy was also observed in the RA subgroup but not in the SLE subgroup. The mean of none of the parameters of FAZ (mm2), Flow Area of Outer Retina (mm2) and Flow Area of Choriocapillaris (mm2) were not statistically significant between the groups Abnormal mfERG and Normal mfERG. (p-value>0.05). Conclusion VD in the DCP layer decreased in abnormal mfERG patients compared to patients with normal mfERG. But it seems that VD in SCP layer, FAZ Area and Flow Area are similar in both groups. OCTA may be used as a non-invasive tool in the diagnosis of early stages of HCQ-induced retinopathy, especially in RA patients, but further studies are needed.
Description of Gemella massiliensis sp. nov., a new bacterium isolated from the human gut.
Authors: Fonkou Maxime Descartes Mbogning , LO Cheikh Ibrahima , Mekhalif Zouina , Bilen Melhem , Tomei Enora , Kuete Edmond , Dubourg Grégory , Raoult Didier , Fenollar Florence , Fournier Pierre Edouard 
Published: March 2021

Abstract
Thanks to its ability to isolate previously uncultured bacterial species, culturomics has dynamized the study of the human microbiota. A new bacterial species, Gemella massiliensis Marseille-P3249 T , was isolated from a sputum sample of a healthy French man. Strain Marseille-P3249 T is a facultative anaerobe, catalase negative, Gram positive, coccus and unable to sporulate. The major fatty acids were C 16:0 (34%), C 18:1n9 (28%), C 18:0 (15%) and C 18:2n6 (13%). Its 16S rRNA sequence exhibits a 98.3% sequence similarity with Gemella bergeri strain 617-93, its phylogenetically closest species with standing in nomenclature. Its digital DNA-DNA hybridization (dDDH) and OrthoANI values with G. bergeri of only 59.7 ± 5.6% and 94.8%, respectively. These values are lower than the thresholds for species delineation (>70% and >95%, respectively). This strain grows optimally at 37°C and its genome is 1.80 Mbp long with a 30.5 mol% G+C content. Based on these results, we propose the creation of the new species Gemella massilienis sp. nov., strain Marseille-P3249 T (= CSUR P3249 = DSMZ 103940).
Identification of Germline Cancer Predisposition Variants During Clinical Ctdna Testing
Authors: Stout Leigh Anne , Kassem Nawal , Hunter Cynthia , Philips Santosh , Radovich Milan , Schneider Bryan 
Published: March 2021

Abstract
Next-generation sequencing (NGS) of circulating tumor DNA (ctDNA) is a non-invasive method to guide therapy selection for cancer patients. ctDNA variant allele frequency (VAF) is commonly reported and may aid in discerning whether a variant is germline or somatic. We report on the fidelity of VAF in ctDNA as a predictor for germline variant carriage. Two patient cohorts were studied. Cohort 1 included patients with known germline variants. Cohort 2 included patients with any variant detected by the ctDNA assay with VAF of 40–60%. In cohort 1, 36 of 91 (40%) known germline variants were identified through ctDNA analysis with a VAF of 39-87.6%. In cohort 2, 111 of 160 (69%) variants identified by ctDNA analysis with a VAF between 40–60% were found to be germline. Therefore, variants with a VAF between 40–60% should induce suspicion for germline status but should not be used as a replacement for germline testing.
Expression and clinical significance of IL7R, NFATc2, and RNF213 in familial and sporadic multiple sclerosis
Authors: Imani Seyedeh Zahra Hosseini , Hojati Zohreh , Dehghanian Fariba , Khalilian Sheyda , Kheirollahi Majid , Khorrami Mehdi , Shaygannejad Vahid , Mirmosayyeb Omid 
Published: March 2021

Abstract
Background

Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disorder of the central nervous system characterized by myelin loss and axonal dysfunction. Increased production of inflammatory factors such as cytokines has been implicated in axon destruction in myelin-less areas. In the present study, we compared the expression level of IL7R, NFATc2, and RNF213 genes in the peripheral blood of 72 MS patients (37 familial MS, 35 sporadic MS) and 74 healthy controls (34 individuals with a family history of the disease, 40 healthy controls without a family history) via Real-time PCR.
Results

Our results showed that the expression level of IL7R was decreased in MS patients versus healthy controls. Also IL7R expression was significantly down-regulated in the sporadic group as compared to other groups. Additionally, there was an increased NFATc2 expression level in MS patients versus healthy controls. Increased expression of NFATc2 in sporadic and familial groups compared to the controls was also seen. Our results represented an increased expression level of RNF213 in the familial patients as compared to the control group. Diagnostic evaluation was performed by ROC curve analysis and area under the curve (AUC) calculation. The correlation of clinical parameters including onset age and EDSS with our gene expression levels were also assessed.
Conclusions

Overall, decreased expression level of IL7R and increased expression level of NFATc2 may be associated with the pathogenesis of MS disease. The RNF213 should be evaluated further for its potential as a candidate biomarker of inflammatory activity in MS in a larger cohort.
Contraindications and Cautions of Foam Rolling – A Delphi Process to Reach Consensus: Study Protocol
Authors: Bartsch Katja , Schleip Robert , Baumgart Christian , Wilke Jan , Slomka Gunda , Turnhöfer Sascha , Egner Christoph , Hoppe Matthias , Klingler Werner , Freiwald Jürgen 
Published: March 2021

Abstract
Background: Foam rolling is a type of self massage using tools such as foam rollers or roller massagers. As no consensus on contraindications and cautions of foam rolling exists to date, current suggestions in the literature are drawn from the field of therapeutic massage. A methodological approach to help closing the gap in the current research landscape is to obtain a reliable opinion consensus of expert groups. The goal of the study is to elaborate experts’ recommendations on the contraindications and cautions for foam rolling techniques by the means of a Delphi process.Methods: The present study includes a preparatory phase as well as three rounds of the actual Delphi process. Academic experts, defined as having (co-)authored at least one scientific paper on foam rolling, are identified via literature search and invited to participate. Consensus on contraindications and cautions of foam rolling will be targeted with a 70 percent agreement after the third round of the Delphi process. Anonymity between participants as well as an iterative approach with controlled feedback constitute key features of the method. Exemplary cases may be reported after round three of the Delphi process in an anonymous manner to supplement the aggregated data.Discussion: Measures to improve panelist recruitment and retention, such as person-to-person cascade approaches and the use of publicly-available information to identify experts are considered to be crucial for the success of the study. Benefits and risks of carrying out the study via online questionnaires need to be considered when interpreting the results. Findings from this Delphi process will provide an overview of the risks related to foam rolling and assist in selecting the scope for future studies.
The analysis of quality of life using SF-36 among patients with myasthenia gravis in South Korea
Authors: Kim Juhyeon , Seok Jin Myoung , Choi Misong , Kim Byoung Joon , Jeong Ansuk , Min Ju-Hong 
Published: March 2021

Abstract
PurposePatients with Myasthenia gravis (MG) have poor health related quality of life (HRQoL), which negatively affects their activities of daily living. The purpose of this study was to analyze factors affecting HRQoL in patients with MG using the 36-Item Short-Form Health Survey (SF-36).MethodsWe prospectively studied patients with confirmed diagnosis of MG. Patients, who presented to the neuromuscular outpatient clinic, at a Samsung medical center in South Korea in a four-month period. Severity of MG was assessed by the Myasthenia Gravis Composite Scale (MGCS) and HRQoL was assessed by the 36-Item Short-Form Health Survey (SF-36) and activities of daily living was assessed by the Korean version of Myasthenia Gravis-specific Activities of Daily Living scale (K-MG-ADL).ResultsA total of 120 patients with MG were invited to participate in the study.We analyzed the difference of SF-36 score according to demographic and clinical characteristics in our MG patients. Significant differences were found according to sex, career change, marital status, MG type, and thymoma.ConclusionBy analyzing the specifics of the HRQoL, the current study demonstrated that this should be reflected in the treatment guidelines for MG patients.
The molecular basis for SARS-CoV-2 recognized by dog ACE2
Authors: Zhang Zengyuan , Zhang Yanfang , Liu Kefang , Li Yan , Lu Qiong , Wang Qingling , Zhang Yuqin , Wang Liang , Liao Hanyi , Zheng Anqi , Ma Sufang , Fan Zheng , Li Huifang , Huang Weijin , Bi Yuhai , Wang Qihui , Gao George F. , Xiao Haixia , Qi Jianxun , Sun Yeping 
Published: March 2021

Abstract
SARS-CoV-2 can infect many domestic animals, including dogs. Herein, we show that dog angiotensin converting enzyme 2 (dACE2) can bind to SARS-CoV-2 spike (S) protein receptor binding region (RBD), and that both pseudotyped and authentic SARS-CoV-2 can infect dACE2-expressing cells. we solved the crystal structure of RBD in complex with dACE2 and found that the total numbers of contact residues, contact atoms, hydrogen bonds and salt bridges at the binding interface in this complex are slightly fewer than those in the complex of the RBD and human ACE2 (hACE2). This result is consistent with the fact that the binding affinity of RBD to dACE2 is lower than that to hACE2. We further show that a few important mutations in the RBD binding interface play a pivotal role in the binding affinity of RBD to both dACE2 and hACE2, and need intense monitoring and controlling.