bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Supported Manganese-Based Coordination Complex as a Nano-Catalyst for the Synthesis of Indazolophthalazinetriones and Investigation of Its Antibacterial Activity
Authors: ariannezhad maryam , habibi davood , heydari somayyeh , Abadi vahideh khorram 
Published: May 2021

Abstract

A new magnetic supported manganese-based coordination complex (Fe3O4@SiO2@CPTMS@MBOL@ Mn) was prepared in consecutive stages, characterized via various techniques (VSM, SEM, TEM, XRD, FT-IR, EDX, TG-DTA and ICP) and used as a capable nano-catalyst for the fast and green synthesis of divers 2H-indazolo[2,1-b]phthalazine-1,6,11(13H)-triones in a simple and efficient procedure from the one pot three-component condensation reaction of aldehydes, dimedone and phthalhydrazide in refluxed ethanol in excellent yields and fast reaction times. The Mn catalyst can be recycled and reused without notable loss of the catalytic activity. Also, the antibacterial properties of the Mn supported nano-catalyst were studied against a number of gram-positive and gram-negative bacterial strains.
Mesenchymal Stem Cells Derived From Human Induced Pluripotent Stem Cells Improve the Engraftment of Myogenic Cells by Secreting Urokinase-type Plasminogen Activator Receptor (uPAR)
Authors: Elhussieny Ahmed , Nogami Ken’ichiro , Sakai-Takemura Fusako , Maruyama Yusuke , Takemura Natsumi , Soliman Wael Talaat , Takeda Shin'ichi , Miyagoe-Suzuki Yuko 
Published: May 2021

Abstract
Background: Duchenne muscular dystrophy (DMD) is a severe X-linked recessive disease caused by mutations in the dystrophin gene. Transplantation of myogenic stem cells holds great promise for treating muscular dystrophies. However, poor engraftment of myogenic stem cells limits the therapeutic effects of cell therapy. Mesenchymal stem cells (MSCs) have been reported to secrete soluble factors necessary for skeletal muscle growth and regeneration. Methods: we induced MSC-like cells (iMSCs) from induced pluripotent stem cells (iPSCs) and examined the effects of iMSCs on the proliferation and differentiation of human myogenic cells and on the engraftment of human myogenic cells in the tibialis anterior (TA) muscle of NSG-mdx4Cv mice, an immunodeficient dystrophin-deficient DMD model. We also examined the cytokines secreted by iMSCs and tested their effects on the engraftment of human myogenic cells.Results: iMSCs promoted the proliferation and differentiation of human myogenic cells to the same extent as bone marrow-derived (BM)-MSCs in coculture experiments. In cell transplantation experiments, iMSCs significantly improved the engraftment of human myogenic cells injected into the TA muscle of NSG-mdx4Cv mice. Cytokine array analysis revealed that iMSCs produced insulin-like growth factor-binding protein 2 (IGFBP2), urokinase-type plasminogen activator receptor (uPAR), and brain-derived neurotrophic factor (BDNF) at higher levels than did BM-MSCs. We further found that uPAR stimulates the migration of human myogenic cells in vitro and promotes their engraftment into the TA muscles of immunodeficient NOD/Scid mice. Conclusions: Our results indicate that iMSCs are new tools to improve the engraftment of myogenic progenitors in dystrophic muscle.
Nurses' Presence at the Patient Bedside: Challenges Experienced by Nurses
Authors: Fallahnezhad Tahereh , Norouzadeh Reza , Samari Banafshe , Ebadi Abbas , Abbasinia Mohammad , Aghaie Bahman 
Published: May 2021

Abstract
Background: Nursing presence is one of the important dimensions of care. The presence of nurses improves the outcome of treatment, self-care, satisfaction and reduces patients' loneliness and anxiety. The literature review shows that most studies have conducted on concept analysis of the nursing presence while nurses face many challenges to present at the patient bedside. The study aimed to identify the challenges of nurses' presence at patients' bedside from the perspective of nurses. Methods: This is a qualitative content analysis study. In-depth semi-structured interviews were conducted with nineteen nurses. Data analysis performed using Zhang's eight stages approach. Results: Three categories emerged: (1) professional challenges with sub-categories: shortage of medical staff, improperly scheduled visits and rounds, time-consuming supervision, inadequate competence, and cultural unfamiliarity, (2) job attenuation with sub-categories: ignoring the needs of nurses, inappropriate management styles (3) unsuitable environment with sub-categories: inadequate medical facilities, ward congestion, and non-standard hospital environment. Conclusions: This study identified the challenges and barriers of nurses' presence at the patient's bedside in different dimensions from the clinical nurses' perspective. Nursing managers must adopt appropriate strategies to solve the challenges of nurses' presence at patients' bedside to achieve the positive benefits of the more effective nurses' presence at patients' bedside.
NAC Transcription Factors ATAF1 and ANAC055 Negatively Regulate Thermomemory in Arabidopsis
Authors: Alshareef Nouf Owdah ,   Annapurna Devi Allu3, Woo Yong H. , de Werk Tobias , Kamranfar Iman , Mueller-Roeber Bernd , Tester Mark , Balazadeh Salma , Schmöckel Sandra M. 
Published: May 2021

Abstract

Pre-exposing (priming) plants to mild, non-lethal elevated temperature improves their tolerance to a later higher-temperature stress (triggering stimulus), which is of great ecological importance. ‘Thermomemory’ is maintaining this tolerance for an extended period of time. NAM/ATAF1/2/CUC2 (NAC) proteins are plant-specific transcription factors (TFs) that modulate responses to abiotic stresses, including heat stress (HS). Here, we investigated the potential role of NACs for thermomemory. We determined the expression of 104 Arabidopsis NAC genes after priming and triggering heat stimuli, and found ATAF1 expression is strongly induced right after priming and declines below control levels thereafter during thermorecovery. Knockout mutants of ATAF1 show better thermomemory than wild type, revealing a negative regulatory role. Differential expression analyses of RNA-seq data from ATAF1 overexpressor, ataf1 mutant and wild-type plants after heat priming revealed five genes that might be priming-associated direct targets of ATAF1: AT2G31260 (ATG9), AT2G41640 (GT61), AT3G44990 (XTH31), AT4G27720 and AT3G23540. Based on co-expression analyses applied to the aforementioned RNA-seq profiles, we identified ANAC055 to be transcriptionally co-regulated with ATAF1. Like ataf1, anac055 mutants show improved thermomemory, revealing a potential co-control of both NAC TFs over thermomemory. Our data reveals a core importance of two NAC transcription factors, ATAF1 and ANAC055, for thermomemory.
Surgical Success Evaluation in Limbal Stem Cell Deficiency Due to Chemical Injury – How to Be Accurate?
Authors: Korkmaz Ilayda , Palamar Melis , Egrilmez Sait , Gurdal Mehmet , Yagci Ayse , Selver Ozlem Barut 
Published: May 2021

Abstract
Purpose: To evaluate limbal stem cell transplantation (LSCT) success in limbal stem cell deficiency (LSCD) due to chemical injury in a tertiary eye care center in Turkey by using ‘Limbal Stem Cell Working Group’ LSCD grading system.Methods: Medical records of 80 eyes of 80 patients who underwent LSCT for LSCD secondary to chemical injury were included. The patients were divided into 3 groups according to performed surgery as limbal autograft, limbal allograft, cultivated limbal epithelial cell transplantation (CLET). Surgical success was defined as improvement in the post-operative 1st year LSCD stage.Results: The mean age of the patients was 37.9±15.7(4-71) with a Male/Female ratio of 2.4. Forty-five(56.3%) patients were injured with alkaline and 16(20%) were injured with acid substance. The mean follow-up time was 60.3±30.6(6-118.6) months. Limbal autograft, allograft and CLET were performed in 58(72.5%), 12(15%) and 10(12.5%) eyes, respectively. The interval between injury and surgery in limbal autograft, limbal allograft and CLET were 43.3±94.1(0.5-592); 14.5±10.6(2.4-32.5) and 122.8±158.9(21.1-504) months, respectively (p=0.02). The overall surgical success rate was 65%. Surgical success rates in each groups were 65.5%, 41.7% and 90%, respectively (p=0.03).Conclusion: It is vital to accurately determine the stage of the LSCD in order to evaluate the surgical success rates. Surgery type and a longer time interval between the chemical injury and surgery seems to be the most important factors associated with a higher surgical success rate. Despite the limited subject number for subgroups, the results were remarkable to emphasize the significance of the novel LSCD grading system.
Optimization of Surgeon Ergonomics With Three-dimensional Heads Up Display for Ophthalmic Surgeries
Authors: Gupta Yogita , Tandon Radhika 
Published: May 2021

Abstract
Purpose: To describe the variables that may be utilized in the optimization of three dimensional heads up surgeries (3D-HUS) for achieving better ergonomics among ophthalmic surgeons. Methods: A cross-sectional study conducted at the operating room of a tertiary eye care centre, equipped with ARTEVO 800 3D surgical microscope and display monitor. The parameters noted were: monitor height (MH), surgeon eye to floor distance (ETFD) surgeon eye to monitor distance (ETMD) (Fig. 1a) and viewing tilt (VT) angle. The neck and eye strain of the surgeon and assistant were scored as per Borg’s CR-10 scale, before and after surgeries.Results: 15 surgeries were analysed. The minimum ETMD was 51 inches and eye strain reduced with shorted ETMD. VT and ETFD was higher for right eye surgeries. The optimum MH was between 50 to 55 inches. Overall, neck strain and eye strain were in the range of 0-3 and 0-1, respectively.Conclusion: The various parameters affecting 3D image quality, neck and eye strain are: chair height, viewing tilt angle, eye centration, monitor distance, laterality of the eye and room illumination.
A novel ANG-BSA/BCNU/ICG MNPs integrated for targeting therapy of glioblastoma
Authors: Lin Fan , Xiong Xiao-Li , Cui En-Ming , Lei Yi 
Published: May 2021

Abstract
Nanomedicine can improve the traditional disease treatment by actively targeting and enhancing the controlled release of drugs in the focus tissue in vivo, moreover the integration of diagnosis and treatment can be achieved by using tracer molecules to indicate the accumulation of nanodrugs in the focus. However ， almost all chemotherapeutic drugs and gene drugs failed to effectively treat glioblastoma (GBM) on account of the existence of blood-brain barrier (BBB) which play a role in GBM. So far, the survival rate of patients with GBM has been hardly improved. In the present study, we constructed an integrated nanoprobe based on albumin nanoparticles (NPs) for targeted diagnosis and treatment of GBM. The nanoprobe consists of albumin-coated superparamagnetic iron oxide (SPIO), Carmustine (BCNU) and indocyanine green (ICG) to achieve bimodal imaging and drug delivery. And the surface-coupled Angopep-2 (ANG, TFFYGGSRGKRNNFKTEEY) polypeptide can specifically bind to low density lipoprotein receptor-related protein (LRP), which is overexpressed in BBB and GBM cells. In the in vitro experiments, we verified that the targeting ability of nanoprobes to GBM cells was significantly better than that of the control group. In addition, in the in vivo experiments, nanoprobes significantly increased the accumulation of brain tumors compared with the control group. Cell killing of GBM cells (U87MG) with ANG-BSA/BCNU/ICG magnetic NPs shows a higher inhibitory effect compared with controls. This novel targeting imaging and drug delivery system provides an efficient strategy for targeted therapy and intraoperative localization of GBM.
Classicalization of Quantum Fluctuations at the Planck Scale in the Rh=ct Universe
Authors: MELIA FULVIO 
Published: May 2021

Abstract
The quantum to classical transition of fluctuations in the early universe is still not completely understood. Some headway has been made incorporating the effects of decoherence and the squeezing of states, though the methods and procedures continue to be challenged. But new developments in the analysis of the most recent Planck data suggest that the primordial power spectrum has a cutoff associated with the very first quantum fluctuation to have emerged into the semi-classical universe from the Planck domain at about the Planck time. In this paper, we examine the implications of this result on the question of classicalization, and demonstrate that the birth of quantum fluctuations at the Planck scale would have been a `process' supplanting the need for a `measurement' in quantum mechanics. Emerging with a single wavenumber, these fluctuations would have avoided the interference between different degrees of freedom in a superposed state. Moreover, the implied scalar field potential had an equation-of-state consistent with the zero active mass condition in general relativity, allowing the quantum fluctuations to emerge in their ground state with a time-independent frequency. They were therefore effectively quantum harmonic oscillators with classical correlations in phase space from the very beginning.
Estimating surface water and vadose water resources for an ungauged inland catchment in Vietnam
Authors: Nguyen Liem Duy , Dang Phuong Dong Nguyen , Nguyen Loi Kim 
Published: May 2021

Abstract
This study aimed to assess water resources for the La Vi catchment, an ungauged inland basin in Vietnam. An Internet of Things-based automatic meteorological station has been installed in the catchment to record hourly weather data from 2016. By comparing water level observations with limited discharge measurements, discharges from November 2015 to February 2018 were calculated at Tan Hoa bridge using the slope-area method. The Soil and Water Assessment Tool was calibrated and validated for the wet season of 2015 and 2017, respectively, using the previously calculated water discharges. Statistical measures including Nash–Sutcliffe index, percent bias, and coefficient of determination indicated the satisfactory performance of the model in simulating water discharge on daily time steps during both periods. The results of the water resources assessment in the catchment showed that the annual average of blue water flow, green water flow, and green water storage reached 1,596.50, 371.13, and 15.36 mm, respectively. The blue water flow reached a higher value in the center of the catchment. Meanwhile, the high-value areas of green water flow and green water storage were in the western upstream and the riverside downstream. These findings could provide a valuable scientific foundation for sustainable watershed management.
Climate change and water supply: governance and adaptation planning in Florida
Authors: Maliva Robert G. , Manahan William S. , Missimer Thomas M. 
Published: May 2021

Abstract
Florida has been described as ‘ground zero’ for climate change in the United States with coastal communities vulnerable to sea-level rise and water supplies under threat from saline-water intrusion, changes in precipitation amounts and patterns, and temperature-driven increases in demands. Water utilities and regional suppliers are responsible for their own water supply plans and adaptation strategies, which are developed largely by a relatively small group of technical specialists (internal and contracted). Water supply planning is prescribed by the state water governance system and local community planning processes. The degree of engagement of large coastal communities and water utilities and regional water suppliers in Florida with climate change research is generally high. Climate change-induced impacts to water supplies and demands over the common 20-year planning horizon are likely to be small relative to increases in demand caused by projected on-going population growth and normal climatic variation. Water utilities in Florida have been incidentally moving toward more climate-resilient supplies (e.g., brackish groundwater desalination) due to the unavailability of additional permittable, inexpensive fresh groundwater rather than climate change concerns. Climate change will narrow the alternatives for future water-supply development.
Energy consumption in anaerobic and aerobic based wastewater treatment plants in Italy
Authors: Ranieri Ezio , Giuliano Silvia , Ranieri Ada Cristina 
Published: May 2021

Abstract
The aim of this study is to carry out an experimental investigation on electricity consumption in wastewater treatment plants in Italy based on aerobic and anaerobic digestion wastewater treatment plants (WWTP). The data refer to plants managed by two major water resources management companies in Italy, Hera and Acquedotto Pugliese (AQP). The survey makes use of statistical tools for data analysis of 202 urban wastewater treatment plants. In order to extend the reliability of the survey, electricity consumption data were analysed through three specific energy demand indicators: kWh/m3, kWh/PE•year, kWh/kgCODremoved referred to each plant. The results show that anaerobic systems are advantageous in terms of electricity consumption per m3 as they achieve more than 50% saving with values ranging from 1.02 kWh/m3 for aerobic plants, instead of 0.43 kWh/m3 for the anaerobic ones. Differences have been found in terms of anaerobic digestion efficiency between the data concerning the plants in northern Italy – Hera Company with an average of 0.33 kWh/m3- and those in Apulia – AQP company with an average of 0.53 kWh/m3. Aerobic systems showed more energy consumption also for HERA-managed WWTP. If anaerobic digestion were implemented on all the AQP WWTP, energy savings should be of approximately 16% and approximately 42% if related to HERA anaerobic-based WWTP average performance.
Elderly Muslim Wellbeing: Family Support, Participation in Religious Activities, and Happiness
Authors: Taufik Taufik , Dumpratiwi Ajeng N. , Prihartanti Nanik , Daliman Daliman 
Published: May 2021

Aims:
The aim of this study was to examine the effect of family support and participation in religious activities on the happiness of elderly muslims.

Background:
An increase in the number of older age people should be balanced with the increased quality of their life. The quality of life is influenced by internal factors such as self-quality, as well as external factors such as family support and religious activities.

Objective:
The objective of this research was to identify differences in the level of happiness of elderly muslims based on several factors: Work, health, salary, Islamic study, and marriage.

Methods:
Three hundred and ten elderly muslims (133 male, 177 female) participated in this study. Respondents were selected by simple random sampling of the elderly muslim population in seven districts in Central Java province, Indonesia.

Results:
The results showed 1) there was a correlation between family support and participation in religious activities and the happiness of elderly Muslims, 2) a healthy elderly person has a higher level of happiness than a sick elderly person; elderly who partake in Islamic studies one to two times a week have higher happiness than the elderly who do not; the retired elderly have the highest happiness compared to the elderly who still work and the elderly who do not (the lowest level of happiness), and there was no difference in the level of happiness in terms of salary and marriage.

Conclusion:
Family support and participation in religious activities are the main predictors of subjective well-being in the muslim elderly. In addition, there are other predictors such as health and participation in Islamic studies.
eHealth in Geriatric Rehabilitation: Systematic Review of Effectiveness, Feasibility, and Usability
Authors: Kraaijkamp Jules J M , van Dam van Isselt Eléonore F , Persoon Anke , Versluis Anke , Chavannes Niels H , Achterberg Wilco P 
Published: May 2021

Background
eHealth has the potential to improve outcomes such as physical activity or balance in older adults receiving geriatric rehabilitation. However, several challenges such as scarce evidence on effectiveness, feasibility, and usability hinder the successful implementation of eHealth in geriatric rehabilitation.

Objective
The aim of this systematic review was to assess evidence on the effectiveness, feasibility, and usability of eHealth interventions in older adults in geriatric rehabilitation.

Methods
We searched 7 databases for randomized controlled trials, nonrandomized studies, quantitative descriptive studies, qualitative research, and mixed methods studies that applied eHealth interventions during geriatric rehabilitation. Included studies investigated a combination of effectiveness, usability, and feasibility of eHealth in older patients who received geriatric rehabilitation, with a mean age of ≥70 years. Quality was assessed using the Mixed Methods Appraisal Tool and a narrative synthesis was conducted using a harvest plot.

Results
In total, 40 studies were selected, with clinical heterogeneity across studies. Of 40 studies, 15 studies (38%) found eHealth was at least as effective as non-eHealth interventions (56% of the 27 studies with a control group), 11 studies (41%) found eHealth interventions were more effective than non-eHealth interventions, and 1 study (4%) reported beneficial outcomes in favor of the non-eHealth interventions. Of 17 studies, 16 (94%) concluded that eHealth was feasible. However, high exclusion rates were reported in 7 studies of 40 (18%). Of 40 studies, 4 (10%) included outcomes related to usability and indicated that there were certain aging-related barriers to cognitive ability, physical ability, or perception, which led to difficulties in using eHealth.

Conclusions
eHealth can potentially improve rehabilitation outcomes for older patients receiving geriatric rehabilitation. Simple eHealth interventions were more likely to be feasible for older patients receiving geriatric rehabilitation, especially, in combination with another non-eHealth intervention. However, a lack of evidence on usability might hamper the implementation of eHealth. eHealth applications in geriatric rehabilitation show promise, but more research is required, including research with a focus on usability and participation.
A Handheld Metabolic Device (Lumen) to Measure Fuel Utilization in Healthy Young Adults: Device Validation Study
Authors: Lorenz Kent Arnold , Yeshurun Shlomo , Aziz Richard , Ortiz-Delatorre Julissa , Bagley James Robert , Mor Merav , Kern Marialice 
Published: May 2021

Background
Metabolic carts measure the carbon dioxide (CO2) produced and oxygen consumed by an individual when breathing to assess metabolic fuel usage (carbohydrates versus fats). However, these systems are expensive, time-consuming, and only available in health care laboratory settings. A small handheld device capable of determining metabolic fuel usage via CO2 from exhaled air has been developed.

Objective
The aim of this study is to evaluate the validity of a novel handheld device (Lumen) for measuring metabolic fuel utilization in healthy young adults.

Methods
Metabolic fuel usage was assessed in healthy participants (n=33; mean age 23.1 years, SD 3.9 years) via respiratory exchange ratio (RER) values obtained from a metabolic cart as well as % CO2 from the Lumen device. Measurements were performed at rest in two conditions: fasting, and after consuming 150 grams of glucose, in order to determine changes in metabolic fuel usage. Reduced major axis regression and simple linear regression were performed to test for agreement between RER and Lumen % CO2.

Results
Both RER and Lumen % CO2 significantly increased after glucose intake (P<.001 for both) compared with fasting conditions, by 0.089 and 0.28, respectively. Regression analyses revealed an agreement between the two measurements (F1,63=18.54; P<.001).

Conclusions
This study shows the validity of Lumen for detecting changes in metabolic fuel utilization in a comparable manner with a laboratory standard metabolic cart, providing the ability for real-time metabolic information for users under any circumstances.