bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruption of Glycogen Metabolism Alters Cell Size in Escherichia Coli
Authors: Walt Felix E. , Bürgy Léo , Engelbrecht Lize , Adams Rozanne C.M. , Stadler Jessica , Strydom Lindi , George Gavin M. , Zeeman Samuel C. , Kossmann Jens , Lloyd James R. 
Published: March 2021

Abstract
Background: The availability of nutrients impacts cell size and growth rate in many organisms. Research in E. coli has traditionally focused on the influence of exogenous nutrient sources on cell size through their effect on growth and cell cycle progression. Utilising a set of mutants where three genes involved in glycogen degradation - glycogen phosphorylase (glgP), glycogen debranching enzyme (glgX) and maltodextrin phosphorylase (malP) - were disrupted, we examined if degradation of this energy storage compound affects cell size. Results: It was found that mutations to malP increased cell lengths and resulted in substantial heterogeneity of cell size. This was most apparent during exponential growth and the phenotype was unaccompanied by alterations in Z-ring occurrence, cellular FtsZ levels and generation times. ∆malP mutant cells did, however, show abnormal nuclear staining patterns and accumulate increased DnaA amounts at late growth stages, indicating a potential effect on DNA replication. Conclusion: This study reveals a link between glycogen metabolism and cell size in E. coli. A disrupted malP allele is associated with cell size heterogeneity and elongation at all stages of growth, without detectable changes to mass doubling time, FtsZ protein levels or division ring frequency.
GOLGA2/GM130 is a novel target of neuroprotection therapy in intracerebral hemorrhage
Authors: deng shu wen , Hu Qing , He Qiang , Chen Xi qian , Lei Qiang , lu wei 
Published: March 2021

Abstract
Background

Impairment of the blood-brain barrier after intracerebral hemorrhage (ICH) can lead to secondary brain injury and aggravate neurological deficits. Owing in part to our lack of understanding of the mechanism of ICH injury to the blood-brain barrier, there are currently no effective methods to prevent or treat it. Here, we explored the effects of Golgi apparatus protein GM130 overexpression or silencing on the blood-brain barrier and neurological function after ICH, to better understand the mechanism involved and facilitate the development of new therapeutic methods.
Results

Levels of the tight junction-associated proteins ZO-1 and occludin decreased, while those of LC3-II, a marker for autophagosomes, increased in hemin-treated Bend.3 cells (p < 0.05). The levels of ZO-1 and occludin increased, while those of LC3-II decreased with GM130 overexpression (p < 0.05). ZO-1 and occludin expression decreased and LC3-II increased after siGM130 transfection, mimicking the effect of hemin (p < 0.05). Tight junctions were disconnected after hemin or siGM130 treatment and repaired with GM130 overexpression. siGM130 transfection in Bend.3 cells increased autophagy flux, whereas GM130 overexpression downregulated autophagy flux. Similar results were verified in an in vivo ICH model. Perihematomal ZO-1 and occludin expression increased, while LC3-II expression decreased in ICH rats (p < 0.05). ZO-1 and occluding expression further decreased and LC3-II expression increased in siGM130-treated ICH rats (p < 0.05), whereas a reverse effect was observed in AAV-GM130-treated ICH rats (p < 0.05). Perihematomal Evans blue and brain water content were much higher in siGM130-treated ICH rats than in the control ICH rats. AAV-GM130-treated ICH rats showed a lower perihematomal Evans blue and brain water content than the control ICH rats.
Conclusions

GM130 overexpression can protect the integrity of the blood-brain barrier from brain injury, inhibit excessive autophagy flux in an ICH in vivo model, and further improve the neurobehavioral prognosis. GM130 overexpression may mediate tight junction protein repair by directly reducing autophagy flux in an ICH in vitro model. GM130 may be a therapeutic target for acute brain injury after ICH.
Ficus retusa L. as possible indicator of air metallic pollution in urban environmentSahli Leila, Belhiouani Hadjer
Authors: Sahli Leila , Belhiouani Hadjer 
Published: March 2021

Abstract
The tree species differed by their capacity to capture air-borne dust and to allocate trace element from contaminated soil. The aim of this study was to explore the accumulation potential of heavy metals (HMs) by F. retusa L. and its possible use for air pollution biomonitoring in urban areas. Plant material was sampled along the national roads in Constantine city (NE-Algeria), characterized by an intense traffic load. The concentrations of cadmium, copper, lead and zinc were determined in the washed and unwashed leaves. The mean concentrations of HMs decrease in the following order: Zn > Pb > Cu > Cd for both studied leaves, and were about 0.68 and 0.98 µg/g for Cd, 7.26 and 8.74 µg/g for Cu, 20.35 and 37.61 µg/g for Pb and 63.33 µg/g and 75.94 µg/g for Zn, for washed/unwashed leaves respectively. The studied metal contents were significantly higher than those cited in the literature; this indicates the traffic road impact on HMs emissions and uptake by plants. Higher values of metal accumulation index (MAI) indicate the effectiveness of the studied species for monitoring air metallic pollution in urban areas, and its usefulness for phytoextraction of HMs from the polluted soils and/or air. Results of this study could be beneficial as preliminary reference values for HMs uptake by F. retusa in urban environments.
Berberine inhibits the proliferation of pancreatic cancer targeting pancreatic cancer stem cells
Authors: Liu Mengmeng , Pan Yue , Tao Xufeng , Li Ning , Li Kun , Xiao Gary Guishan 
Published: March 2021

Abstract
BackgroundPDAC is universally acknowledged to be one of the highest mortality rate of cancer-related deaths. PCSCs, regulated by EMT, could promote the proliferation of PDAC. Berberine with high medicinal value has usually been used as an anti-cancer agent. Hence the purpose of this study is to investigate the anti-cancer effect of berberine in PDAC. MethodsMTT assay was used to verify berberine inhibiting the proliferation of PDAC. Immunofluorescence staining, stem cell sphere, wound healing and transwell migration assay were demonstrated the anti-proliferation and anti-stemness of PCSCs in vitro . PANC-02 cells were injected in C57BL/6 mice to establish the orthotopic pancreatic-cancer model in vivo . H&E and Ki67 immunohistogical staining assay were used to evaluated the effect of berberine in PDAC in vivo. q-PCR and Western blot methods were applied to detect the expression of EMT procedure.ResultsIn this study, berberine has selective anti-cancer effect in PDAC in vitro . Moreover, berberine suppressed the proliferation and stemness of PCSCs in PDAC. In vivo , berberine reduced the tumor size and decreased the expression of Ki67 in orthotopic pancreatic-cancer pancreases. In addition, berberine inhibit the EMT signaling pathway both in vitro and in vivo . ConclusionsOur study indicates that berberine inhibit the proliferation of PDAC in vivo and vitro . The mechanism of anti-cancer effect on berberine may suppress the PCSCs through inhibiting EMT procedure. Therefore, berberine may be the novel antineoplastic drug with clinical effectiveness in PDAC. Keywords: Berberine, PDAC, PCSCs, EMT, berberine
Clinical Features, Prognosis and Susceptibility Genes of 2 Infants With Rhabdomyosarcoma of the Tongue: Case Report
Authors: Yang Peiyi , Xu Na , Su Yan , Duan Chao , Wang Shengcai , Fu Libing , Yu Tong , Guo Ruolan , Ma Xiaoli 
Published: March 2021

Abstract
Background Rhabdomyosarcoma(RMS) is the most common soft tissue tumor in children. RMS of the oral cavity accounts for 10–12% of all head and neck RMS, and the tongue is a rare primary site. The most common pathological types of RMS include embryonal rhabdomyosarcoma and alveolar rhabdomyosarcoma, and spindle cell rhabdomyosarcoma(SRMS) is rare. Meanwhile, it has been reported in the literature that infant is a rare onset age in RMS. We review two cases of infant tongue SRMS, and analyze the clinical features, prognosis and susceptibility genes. Case presentation The clinical data of 2 infants with RMS of the tongue admitted to Beijing Children's Hospital were retrospectively collected. Both the children and their parents accepted exome-customized capture sequencing of single gene disease in peripheral venous blood, and the results were analyzed. Both cases were infants and diagnosed with lingual RMS at 3 and 5 months after birth, respectively. The pathological type were both spindle cell rhabdomyosarcoma. The maximum diameter of the tumor in case 1 was 8mm, and 23mm in case 2. The clinical TNM staging both were T1aN0M0. IRS staging of case 1 was II, and case 2 was I. They were both classified in the low risk group, and treated with surgery and chemotherapy. The regimen of chemotherapy including 4 courses of VAC(vincristine, actinomycin D, cyclophosphamide) regimen first, followed by 4 courses of VA(vincristine, actinomycin D) regimen. Case 1 was in complete remission at the latest follow-up, and case 2 suffered from relapse 10 months after stopping chemotherapy, and achieved complete remission after the multi-disciplinary treatment of chemotherapy, surgery and radiotherapy. The venous blood gene test of the two children did not find pathogenic mutation or possible pathogenic mutation related to RMS. In case 1, variants of CDK4 and BRCA1 gene, both unclear significance (possibly) related with RMS, were detected. For case 2, we found three variants of unknown significance that were (possibly) associated with RMS, including variants of TRIP13, APC, and RAD54L gene. Conclusions Infant tongue RMS is rare, the clinical manifestations of which lack specificity, and early recognition is difficult. The success and timing of local treatment are important prognostic factors. Genetic testing may be helpful for early detection of tumor susceptibility and prognosis estimation.
A Study of anxiety and fear level in dental practitioners from coronavirus and need of clinical practice modification to combat Covid -19
Authors: Bharat Rakhi , Jagnade Rinku , Katare Gopal , Daga Sonal 
Published: March 2021

An outbreak of novel corona virus (COVID-19) in China has influenced several aspects of the life of healthcare professionals, especially dentists, who are actually exposed to a higher risk of getting infected due to close interaction with their patients during treatments.
The study was conducted to understand the anxiety and fear level of dental practitioners in getting infected during practice in the current corona virus (COVID-19) situation. In addition, also to evaluate their awareness about various practice modifications needed or required to combat COVID-19 during clinical practice.
A cross-sectional study was conducted by an online survey from 3rd Aug to 8th Sept 2020. For the central region, a well-defined survey was planned at Google doc. A total of 239 participants from 16 different states of India had responded. Post scrutiny, completed questionnaires (n = 210) were included in the study. Statistical analysis was performed using SPSS version 25. Chi-Square and tests were applied
The fear and anxiety levels of dentists are found as; 69% of participants were afraid getting infected from a patient visiting their clinics. 74.8% were scared while providing treatment to patients. 47.1% felt suggest or think not to do practice until the number of COVID-19 patient cases decreases.54.3% participants felt anxiety and scared while interacting to patients while treatments, 81% have fear to carry the infection from clinic to their home and can get their family infected. 40% were afraid of getting quarantined and 59.5% were concerned about the cost of treatment on the off chance if get Infected. Dental practitioners are in a state of anxiety and fear while treating their patients due to the pandemic impact around the community. A number of dental practitioners have either modified their clinical practice process as per recommended guidelines for emergency treatment only, or closed down clinics for an uncertain period.
The effect of 5% NaOCl and 17% EDTA, at 24hrs and 8days, on the microhardness of MTA, Biodentine and Pozzolan cement: An in- vitro study.
Authors: Dasani Sarita , Hora Baljeet S , Ajmera Rucheeta S , Gupta Brijesh , Dondulkar Yadnesh , Kosta Suparna 
Published: March 2021

Aim & Objectives: The aim of this study was to evaluate the effects, at 24 h and 8 days, of 5% NaOCl and 17% EDTA on the Vicker’s microhardness of Mineral Trioxide Aggregate(MTA Angelus) (MTAA), Biodentine(Septodont, Saint Maur des Fosse’s France) and Pozzolan based endodontic cement named Endocem MTA(Maruchi, Wonju, Korea).
Materials and method: Sixty samples of MTAA, Biodentine and Endocem MTA were tested for baseline microhardness at 24 h. They were divided into 12 subgroups (5% NaOCl or 17% EDTA, 24 h and 5% NaOCl or 17% EDTA at 8 days) and microhardness was evaluated at different time points. Results were recorded and analysed statistically via one-way ANOVA and Tukey’s post hoc test.
Results: MTAA had a higher baseline microhardness than both biodentine and Endocem MTA. At 24 hrs, the microhardness of all the materials was reduced by NaOCl and EDTA. At 8 days, NaOCl reduced the microhardness of MTA but that of Biodentine and Endocem MTA was increased. EDTA at 8 days, reduced the microhardness of both MTAA and Biodentine but an increase was seen with Endocem MTA.
Conclusion: Changes in microhardness of MTAA, Biodentine and Pozzolan cement(Endocem MTA) were associated with the time for which the materials are allowed to set as well as the irrigating agent used,.
The association of oral health-related behaviour and dental caries among 6 -7 year old school children of Wilsonia Pakwara Moradabad : Across sectional study
Authors: Abdullah Sabzar , Khazir Mahbooba 
Published: March 2021

Abstract
Aim: To find out the association between oral health related behavior and dental caries

Methods: A cross-sectional study was conducted on the 6-7-year-old students of Wilsonian school, DFT was clinically assessed at the first visit and a validated questionnaire was distributed among the parents of the students. The questionnaire consists of the three main questions, one regarding the tooth brushing Frequency that was assessed by the following question: “How many times a day does your child brush his/her teeth?” with the answers of “twice or more/day,” “once/day,” “less than once/day,” and “do not know.” These answers were recorded as “twice or more/day; coded 1” and “less than twice/day (including ‘do not know’); coded 2”. Another question regarding the drinking sugar-sweetened beverages frequency was assessed by the following question: “How often does your child drink sugar-sweetened beverages?” with the answers of “several times/month or never; coded 1,” “once/week; coded 2,” “2- 3 times/week; coded 3,” “4-6 times/week; coded 4,” “once/day; coded 5” and “twice or more/day; coded 6.” .The Third question was regarding “Snack-eating habits” that was assessed by the following question: “When does your child eat snacks?” with the answer of “does not eat snacks; coded 1,” “eats snacks at a set time; coded 2” and “eats snacks freely whenever he/she wants; coded 3.”
Results: Poor oral behavior was associated with higher DFT after adjusting for all covariates Toothbrushing frequency, frequency of drinking sugar-sweetened beverages and snack-eating habits (OR=1.49,CI-1.17-1.89) (OR=2.01,95% CI=1.27,3.18) and (OR=1.83,95%CI=1.14,2.92) associated with DFT,
Conclusions: improving children’s oral health behavior might decrease their dental caries
by Strengthening their self-control.
KEYWORDS
Child, child dentistry, dental caries, oral health behavior
Analysis of the biological efficiency and quality factors of mushrooms of the genus Pleurotus (Fr.) P.Kumm as a model of effective cultivation of lignicolous fungi with high functional value
Authors: І. І. Бандура  , А. С. Кулик  , Н. А. Бісько  , О. В. Хареба  , О. М. Цизь  , В. В. Хареба  
Published: March 2021

Purpose. Morphological and organoleptic evaluation of oyster mushroom strains for selection of highly productive cultivars assortment with valuable consumer properties for winter and summer cultivation and suitability for sale in fresh or processed form were investigated.Methods. The scheme of the experiment included six strains of oyster mushrooms belonging to two species: Pleurotus ostreatus (5 strains: 2301, Z, 2316, 2456, 431) and P. pulmonarius (2314). Laboratory, laboratory-industrial and statistical methods are used.Results. The dynamics of agrochemical parameters of substrates obtained by the method of aerobic fermentation in the high layer proved their optimality according to the main criteria and compliance to regulatory documentation. The biological efficiency of the strains was in the range of 40–78.9%. The highest weight of clusters was found in the “summer” strain 431 (430.7 g). The highest weight of the fruiting body was determined for the strain 2301 (15.2 g), the lowest – for the 2456 (3.3 g).Conclusions. The values of biological efficiency of strains was determined. The highest average values for the first flush of fruiting had strains 2316 (78.9%) from the “winter” group (A) and 431 (78.4%) from the “summer” group (B). According to the results of statistical analysis, significant differences were revealed between the studied strains in the main morphological indicators of clusters. It was found that the mass of clusters of «winter» cultivars is much less dependent on the strain type (220.8–273.4 g) than that of «summer» cultivars (83.4–430.7 g). The index of the asymmetry coefficient of the cluster, which can be useful for calculating the required container sizes was proposed. It was determined that the morphological features of fruiting bodies had significant differences in all studied parameters. In particular, the A group strains differed in size and weight. It was proposed the index of weight loss of the crop coefficient, which shows the ratio between the cap and stipe of the fruiting body and make it possible to predict the amount of mushroom raw material that will be sold in the form of caps, and the amount of raw material that can be processed into minced mushroom, powder and others. The best coefficient was obtained for the fruiting bodies of strain 2314 (0.87), and the worst – for 2456 (0.59), which, accordingly, is not recommended for sale by individual fruiting bodies. It was found that the fruiting bodies of high-yielding strains 2316 and 431 had a number of organoleptic defects.
Characteristics of productivity of soft winter wheat samples from Common Bunt-Resistant Nursery (CBUNT-RES) in the southern Forest-Steppe zone of Ukraine
Authors: С. М. Холод  , В. М. Кір’ян  , Р. С. Вискуб  
Published: March 2021

Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them.Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivi­ty were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening.Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight.Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity.
O LUGAR DO DESENVOLVIMENTO NA MOBILIZAÇÃO SOCIAL: Signos de Desenvolvimento e produção de utopismos nas Jornadas de Junho de 2013
Authors: Santos Gustavo Souza , Marília Pereira Anete 
Published: March 2021

Manifestações populares e mobilizações sociais carregam explicitamente uma carga de demandas pelas quais o objeto de luta e querela se anela. Tais demandas possuem dimensões múltiplas e acenam, a partir de uma tensão pela meta, lacunas que reclamam preenchimento e cenários que precisam de transformação, considerando a narrativa dos que se mobilizam. Essas demandas comumente disputam necessidades de desenvolvimento(s), atreladas à signos de progresso, promessas sistematizadas e ensejos por um lugar ideal no qual o bem viver e as benesses da modernidade se realizem como plenitude dos modos de produzir e operar a vida social. Essa elucubração da luta produz utopismos diante do desenvolvimento. Desse modo, a proposta deste estudo foi refletir a relação entre os signos de desenvolvimento e produção de utopismos nos objetos e ideais das mobilizações sociais, tendo por quadro de análise as Jornadas de Junho de 2013 no Brasil. Metodologicamente, considerou-se um estudo de caso por meio de análise documental de jornais impressos apurados com maior tiragem no período, considerando como corpus a cobertura jornalística do período a partir das categorias de demandas emergentes dos atos. Esse novo desenho de articulação popular indica uma flexão da participação, onde as territorialidades de insurgência são tessituras de coalizão e desenvolvimento(s). A ressonância da voz reivindicatória popular em rede indica a subversão das assimetrias do desenvolvimento ou, pelo menos, um sinal de que a planificação do conceito encontra impasses maiores. Microdesenvolvimento é o início de uma discussão possível sobre o quadro atual de ativismos em rede e seu significado ainda provocador acadêmica, política e socialmente.
MICROCRÉDITO E EMPREENDEDORISMO: Uma análise da Situação dos Beneficiários do Microcrédito da Cidade De Xai-Xai, Gaza, Moçambique
Authors: Rosario Nelson Maria , France Daniel Mutemba , Muchanga Aurélio Ernesto 
Published: March 2021

O presente artigo analisa a importância que as instituições de microcrédito têm no fomento de atividades empreendedoras da cidade de Xai-Xai, província de Gaza. Para o alcance do objetivo preconizado o estudo teve uma abordagem qualitativa e quantitativa, sendo a entrevista e inquéritos as técnicas usadas para a recolha dos dados. Foram inqueridos catorze beneficiários do microcrédito, sendo o Fundo de desenvolvimento da Mulher (FDM) e a Socedade de Créditos de Moçambique (SOCREMO) as instituições fornecedoras do microcrédtio, ambos sediados na cidade de Xai-Xai. A pesquisa constatou que a procura de acesso a microcrédito deve-se principalmente a falta de emprego e a busca de fonte de rendimento. Os resultados da pesquisa mostraram que muitos dos beneficiários investiram ou apostaram em pequenos negócios (abertura de lojas de venda de produtos alimentares, de higiene, roupa, calçados, bares, etc) para minimizar a situação do desemprego e geração de renda.
Palavras-chaves: microcrédito, empreendedorismo e Xai-Xai.