bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mealtime situations in nursing homes from the residents’ perspective – an integrative review
Authors: Harstäde Carina Werkander , Andersson Stefan , Lagerbielke Erika , Sandgren Anna , Heikkilä Kristiina 
Published: February 2025

Abstract

Background
When moving to a nursing home, the new resident also meets a new kind of culture, including communal, shared meals. Gaining greater insight into the resident experience of mealtimes in nursing homes is essential to identify the meaning that mealtime situations have and highlight the potential barriers and facilitators to the implementation of mealtime situations that create wellbeing for residents. The aim of this integrative review was therefore to shed light on mealtime situations in nursing homes from the residents’ perspective.

Methods
The literature search was performed using a combination of MeSH keywords and free text terms in ASSIA, CINAHL, PsycInfo, PubMed, and Web of Science. After scrutinizing the articles and quality checks, 13 articles were chosen. The analysis was performed following Whittemore and Knafl’s instructions for integrative reviews.

Results
The experiences of the mealtime situations were partly connected to residents’ personhood and partly to the nursing home’s organization of the mealtimes. Three main categories emerged: mealtimes as a source of well-being in nursing home life, mealtimes (re)creating continuity in life and preserving identity, and mealtimes as a balancing act between autonomy and need of support. Residents in nursing homes want the possibility to choose both what to eat, with whom they will eat and where they eat. The mealtimes in nursing homes and how they are constructed have an important role in residents’ feelings of having control over their life situation and can also strengthen residents’ identity and autonomy.

Conclusion
Staff needs to be aware of the meaning of mealtimes to promote person-centered care in regard to meals. Further research is needed to explore how different mealtime situations have an impact on nursing home residents’ lives as well as on the care the staff gives.
Childlessness and its associated factors among Chinese women: a nationwide population-based study
Authors: Han Qing , Dang Junhua , Wu Yibo , Ye Wenting 
Published: February 2025

Abstract

Background
China has experienced a continuous decline in fertility rates, despite the end of the one-child policy, further intensifying population ageing. Timely evidence on the factors associated with childlessness among Chinese women is needed, alongside examining reproductive anxiety among childless women of reproductive age.

Methods
This nationwide, cross-sectional study was based on the 2023 Psychology and Behavior Investigation of Chinese Residents Survey. A total of 10,802 women across mainland China who were (or had been) married were included, of whom 5,885 were of reproductive age (20–49 years) and 4,917 of post-reproductive age (≥ 50 years). Logistic and linear regression analyses were conducted to examine factors associated with childlessness among reproductive-aged and post-reproductive women, and reproductive anxiety among childless women of reproductive age at intrapersonal, interpersonal, and societal levels, as outlined in the social-ecological model.

Results
The comparison of reproductive patterns among women across different age groups showed a dramatic decline in the proportion of having three or more children, and a gradual increase in the childlessness over time. Among 5,885 women of reproductive age by the time of the survey, 612 (10.4%) reported having no children, while among 4,917 post-reproductive women, 161 (3.3%) were childless. Higher education level (OR = 2.83 [2.26, 3.55]; OR = 2.35 [1.44, 3.74]), being the only child in the family (OR = 2.85 [2.28, 3.55]; OR = 10.56 [7.38, 15.14]), and exposure to intimate partner violence (OR = 1.15 [1.02, 1.29]; OR = 1.68 [1.43, 1.98]) were significantly associated with childlessness among both reproductive-aged women and post-reproductive women. Poorer stress coping ability and exposure to sexual abuse during childhood were also associated with childlessness in post-reproductive women. Among childless women of reproductive age, higher education level, higher body image dissatisfaction, exposure to child abuse, parental issues during childhood, and belonging to ethnic minority groups were associated with increased reproductive anxiety.

Conclusions
This study used the most recent nationwide survey data from China to identify factors associated with childlessness and reproductive anxiety, with an emphasis on socio-economic factors and domestic violence. The findings highlight the significant impact of domestic violence on fertility behaviors and reproductive anxiety, providing valuable evidence for future policy interventions.
Cell-free expression system: a promising platform for bacteriophage production and engineering
Authors: Nour El-Din Hanzada , Kettal Maryam , Lam Serena , Granados Maciel José , Peters Danielle L. , Chen Wangxue 
Published: February 2025

Abstract
Cell-free expression is a technique used to synthesize proteins without utilising living cells. This technique relies mainly on the cellular machinery —ribosomes, enzymes, and other components — extracted from cells to produce proteins in vitro. Thus far, cell-free expression systems have been used for an array of biologically important purposes, such as studying protein functions and interactions, designing synthetic pathways, and producing novel proteins and enzymes. In this review article, we aim to provide bacteriophage (phage) researchers with an understanding of the cell-free expression process and the potential it holds to accelerate phage production and engineering for phage therapy and other applications. Throughout the review, we summarize the system’s main steps and components, both generally and particularly for the self-assembly and engineering of phages and discuss their potential optimization for better protein and phage production. Cell-free expression systems have the potential to serve as a platform for the biosynthetic production of personalized phage therapeutics. This is an area of in vitro biosynthesis that is becoming increasingly attractive, given the current high interest in phages and their promising potential role in the fight against antimicrobial resistant infections.
Microglial depletion decreases Müller cell maturation and inner retinal vascular density
Authors: Rowthorn-Apel Nathaniel , Vridhachalam Naveen , Connor Kip M. , Bonilla Gracia M. , Sadreyev Ruslan , Singh Charandeep , Gnanaguru Gopalan 
Published: February 2025

Abstract

Background
The neuroretinal vascular system is comprised of three interconnected layers. The initial superficial vascular plexus formation is guided by astrocytes around birth in mice. The formation of the deep and intermediate vascular plexuses occurs in the second postnatal week and is driven by Müller-cell-derived angiogenic signaling. Previously, we reported that microglia play an important role in regulating astrocyte density during superficial vascular plexus formation. Here, we investigated the role of microglia in regulating Müller-cell-dependent inner retinal vascular development.

Methodology
In this study, we depleted microglia during retinal development using Csf1R antagonist (PLX5622). We characterized the developmental progression of inner retinal vascular growth, effect of microglial depletion on inner retinal vascular growth and Müller cell marker expressions by immunostaining. Differential expressions of genes in the control and microglia depleted groups were analyzed by mRNA-seq and qPCR. Unpaired t-test was performed to determine the statistical differences between groups.

Results
This study show that microglia interact with Müller cells and the growing inner retinal vasculature. Depletion of microglia resulted in reduced inner retinal vascular layers densities and decreased Vegfa isoforms transcript levels. RNA-seq analysis further revealed that microglial depletion significantly reduced specific Müller cell maturation markers including glutamine synthetase, responsible for glutamine biosynthesis, necessary for angiogenesis.

Conclusions
Our study reveals an important role for microglia in facilitating inner retinal angiogenesis and Müller cell maturation.
The stability and self-assembly of tri-calcium silicate and hydroxyapatite scaffolds in bone tissue engineering applications
Authors: Beheshtizadeh Nima , Seraji Amir Abbas , Azadpour Behnam , Rezvantalab Sima 
Published: February 2025

Abstract
The fabrication of scaffolds for bone tissue engineering (BTE) applications often involves the utilization of two distinct categories of biomaterials, namely calcium phosphates and calcium silicates. The selection of these materials is based on their biocompatibility, bioactivity, and mechanical characteristics that closely resemble those of natural bone. The present research examined the utilization of hydroxyapatite (HAP) and tri-calcium silicate (TCS), which are among the most commonly utilized materials in calcium phosphates and calcium silicates, in the context of bone scaffolding applications. A molecular dynamics simulation was conducted to investigate the impact of different concentrations of ceramic nanoparticles, when combined with sodium alginate (SA) hydrogel, on the fabrication of bone scaffolds.
The stability and self-assembly were assessed through several parameters, such as the solvent-accessible surface area (SASA), radius of gyration (Rg), radial distribution function (g(r)), root-mean-square deviation (RMSD), root-mean-square fluctuation (RMSF), hydrogen bonding, van der Waals, electrostatic, and total energies. The findings indicate that the addition of 10 wt% HAP and TCS to the SA hydrogel matrix results in a more compact, stable, and potentially less hydrated structure. Accordingly, the experimental validation of these simulation approved our in silico findings. Experimental rheology and mechanical properties evaluation validate our simulation results, indicating a superior characteristic of TCS10 and HAP10 inks and 3D-printed scaffolds among other composition ratios. This could potentially benefit the in vitro and in vivo performance of the scaffold and its interaction with cells. The aforementioned traits are considered fundamental for the successful execution of the scaffold in the field of BTE. The findings indicate that TCS samples exhibit superior properties when compared to HAP samples, specifically in terms of composition with SA hydrogel.
Traceability of oak (Quercus petraea (Matt.) Liebl. and Quercus robur L.) logs: the Biomtrace database
Authors: Longuetaud Fleur , Mothe Frédéric , Martinetto Dorian , Ngo Phuc , Piboule Alexandre , Rittié Daniel , Bordat Frédéric , Jacquin Philippe , Debled-Rennesson Isabelle , Albert Aymeric , Richter Claudine 
Published: February 2025

Abstract

Key message
The Biomtrace database contains 33,390 RGB images of the butt end cross-section of 5135 French oak logs. Each log was photographed several times with different camera orientations during an initial shooting session. For more than half of the logs, additional photos were taken at least 3 weeks after the first photo session. Cross-sections were segmented on all the images using the PointRend convolutional neural network. Spatial calibration was carried out by a specific algorithm using a checkerboard pattern present in each image. This image database was created with the aim of developing algorithms for the biometric traceability of logs, involving artificial intelligence approaches that require large databases. But other applications are also possible, such as the automatic extraction of information on the size and quality of logs. The Biomtrace database is available at https://doi.org/10.57745/9DBCL4, and associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/feda0a0e-041a-4190-9a73-5159b10ff0f0.
Tribological behavior of unfilled PTFE under static loading in dry sliding condition: a Taguchi-ANN perspective
Authors: Chaudhari Kiran Ashokrao , Bhangale Jayant Hemchandra 
Published: February 2025

Abstract
This work explores the friction and wear characteristics of unfilled polytetrafluoroethylene (PTFE) operating in static unlubricated sliding conditions using Taguchi analysis. The research uses a design of experiment (DOE) technique, focused on sliding velocity, and applied pressure and sliding time as parameters. Systematic experimentation is facilitated with Taguchi’s L9 orthogonal array, and Minitab 17 software is used to evaluate the findings. Signal-to-noise ratios (SNR) are used in the evaluation of individual parameter effects, the creation of regression models, and the establishment of ideal operating conditions. The analysis focuses on predicting wear (W), specific wear rate (Ws), and friction coefficient (f) through regression and ANN (artificial neural network) models, with ANN demonstrating better performance. The results advocate for optimal operating condition for PTFE under static load. This study adds important information for sectors where PTFE is employed as a primary material, such as rolling and sliding contact bearings.
Prevalence of hypertensive retinopathy and its associated factors among adult hypertensive patients attending at Comprehensive Specialized Hospitals in Northwest Ethiopia, 2024, a multicenter cross-sectional study
Authors: Gudayneh Yitayal Abebe , Shumye Abebech Fikade , Gelaye Abebech Tewabe , Tegegn Melkamu Temeselew 
Published: February 2025

Abstract

Introduction
Hypertensive retinopathy refers to changes in the retinal microvasculature resulting from elevated blood pressure, and the global burden ranges from 2 to 85%. However, there was no evidence on prevalence and associated factors of hypertensive retinopathy among adult hypertensive patients in the study area even in Ethiopia.

Objective
The aim of this study was to investigate prevalence of hypertensive retinopathy and associated factors in adult hypertensive patients attending at Comprehensive Specialized Hospitals in Northwest Ethiopia in 2024.

Methods
Multicenter hospital-based cross-sectional study was conducted in Northwest Ethiopia Comprehensive specialized hospitals from June, 07 to August, 07, 2024. A multistage sampling technique with an interval of 3 was applied to select 696 study participants. Data were collected through personal interviews, review of medical records and eye examinations. Data were entered into the Kobo Toolbox and then transferred to STATA version 17 for analysis. Bivariable and then multivariable binary logistic regression models were fitted to determine factors associated with hypertensive retinopathy. Variables with a P-value of less than 0.05 at multivariable analysis were considered statistically significant.

Result
A total of 696 (95.34%) participants were included in the study. The prevalence of hypertensive retinopathy was 57.47%(95%CI: 53.75, 61.10). Age > 74 years (AOR = 4.24, 95%CI = 1.54,11.64), heart disease(AOR = 5.38, 95%CI = 1.86,15.58), duration of hypertension > 5years (AOR = 12.66, 95%CI = 3.88,41.29), dyslipidemia (AOR = 3.44,95%CI = 1.59–7.45), uncontrolled current levels of hypertension (AOR = 40.03, 95%CI = 17.19,93.18), poor adherence of hypertensive medications (AOR = 1.84, 95%CI = 1.12,3.03) and diabetes (AOR = 3.56, 95%CI = 1.49,5.99) were positively associated with hypertensive retinopathy.

Conclusion
-The prevalence of hypertensive retinopathy is high among systemic hypertensive patients seen in Northwest Ethiopia comprehensive specialized hospitals and independently associated with older age, longer duration of hypertension, heart disease, diabetes, dyslipidemia, poor adherence of hypertension medications and uncontrolled hypertension. Early diagnosis and treatment of hypertension was recommended to prevent target organ complications.
Effect of vibration therapy on upper limb range of motion and muscle strength in children with hemiplegia: a randomized controlled trial
Authors: Heneidy Walaa E. , Farouk Mahmoud Adel , Olama Khaled A. , Ibrahim Amira F. 
Published: February 2025

Abstract

Background
Children with hemiplegic cerebral palsy (HCP) suffer from muscle weakness and restricted joint flexibility in the involved upper limb. This affects their performance in everyday tasks. Therefore, the current study aimed to investigate the effect of vibration therapy (VT) on upper limb range of motion (ROM) and muscle strength in these children.

Methods
The study included 30 children from both sexes aged 3 to 6 years who were diagnosed with HCP. Subjects were recruited from the outpatient clinic, Department of Physical Therapy, Qanater Central Hospital, Qalyubia, Egypt. Participants were randomly assigned to control group A (n = 15) and participated in the designed physical therapy programme for 1 h, while study group B (n = 15) completed the same programme for 30 min in addition to VT administered via whole-body vibration (WBV) device on upper limb for another 30 min, three sessions/week for four successive weeks. The upper limb ROM was assessed by the digital goniometer and muscle strength by Lafayette handheld dynamometer. Statistical SPSS package program version 25 for Windows (SPSS, Inc., Chicago, IL, USA) was used, paired t-test was applied to investigate within-group changes, and unpaired t-test was used to compare the study group and control at pre- and post-treatment.

Results
The children in the study group achieved superior improvements in ROM of shoulder abduction (8.25%), forearm supination (35.76%), wrist flexion (34.62%), and wrist extension (14.03%) compared to the control group. They also had improved muscle strength of shoulder abductors (53.94%), wrist flexors (47.06%), and wrist extensors (40.24%) at post-treatment compared to baseline than children in the control group.

Conclusions
Combining VT using the WBV device with the PT programme showed higher percentages of improvements in the ROM for shoulder abduction, forearm supination, and wrist flexion and extension, as well as muscle strength in shoulder abductors and wrist flexors and extensors, in children with HCP compared to the PT programme alone indicating the clinical benefit of integrating the WBV within PT programme in rehabilitation of these children.

Trial registration
Effect of whole-body vibration on upper limb range of motion and strength in children with hemiparetic cerebral palsy, ID NCT05896111. Registered 7 June 2023 — retrospectively registered.
Feature-level analysis of a novel smoking cessation program integrating app-based behavioral therapy and an electronic cigarette
Authors: Schiek Helen , Esch Tobias , Hoetger Cosima 
Published: February 2025

Abstract

Background
Smoking cessation apps and electronic cigarettes have emerged as potentially effective tools for smokers motivated to quit. Nuumi, a novel intervention, integrates app-based behavioral therapy and an electronic cigarette. Using secondary data from a single-arm pilot trial evaluating the intervention, we investigated the degree to which users engaged with the program’s features, and the relationships between feature utilization and smoking abstinence during the first 8 weeks of the trial. Features included (1) behavioral therapy lessons, (2) meditation audios, (3) a toolbox, (4) progress tracking, and (5) an electronic cigarette. Outcomes were self-reported 7-day point prevalence abstinence from smoking 4 weeks and 8 weeks after program initiation. Self-reported smoking abstinence data and app utilization data of 62 participants were analyzed. Multiple univariate logistic regressions were performed with total program engagement and feature utilization as predictor variables, and 4-week and 8-week post-baseline smoking abstinence rates as dependent variables.

Results
Four weeks and eight weeks after program initiation, 35% (n = 22) of participants reported abstinence. Higher total engagement predicted higher likelihood of abstinence at 4 weeks (OR = 2.73, 95% CI [1.53, 5.70], p = 0.002), and 8 weeks follow-up (OR = 1.52, 95% CI [1.10, 2.22], p = 0.017). Four weeks after program initiation, significant feature-level predictors of smoking cessation included the number of therapy lessons completed (OR = 1.13, 95% CI [1.04, 1.24], p = 0.010), meditations completed (OR = 1.54, 95% CI [1.11, 2.30], p = 0.025), the number of times the progress tracking feature was accessed OR = 1.02, 95% CI [1.00, 1.03], p = 0.036), and the number of days the electronic cigarette was used (OR = 1.30, 95% CI [1.21, 1.58], p = 0.010). Eight weeks after initiation, only electronic cigarette use was predictive of smoking abstinence (OR = 1.06, 95% CI [1.02, 1.12], p = 0.048).

Conclusion
The intervention may have potential to support smokers motivated to quit. With respect to program features, use of the electronic cigarette may promote sustained cigarette abstinence; however, the association between behavioral therapy features and smoking abstinence may disappear over time. Measures to increase user engagement should be explored, and future research should test the intervention using a randomized controlled trial.

Trial registration
German Clinical Trials Register DRKS00032652, registered prospectively 09/15/2023.
Machine learning approaches for image classification in developmental biology and clinical embryology
Authors: Mapstone Camilla , Plusa Berenika 
Published: February 2025

ABSTRACT
The rapid increase in the amount of available biological data together with increasing computational power and innovative new machine learning algorithms has resulted in great potential for machine learning approaches to revolutionise image analysis in developmental biology and clinical embryology. In this Spotlight, we provide an introduction to machine learning for developmental biologists interested in incorporating machine learning techniques into their research. We give an overview of essential machine learning concepts and models and describe a few recent examples of how these techniques can be used in developmental biology. We also briefly discuss latest advancements in the field and how it might develop in the future.