bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaccine‐related major cutaneous reaction size correlates with cellular‐mediated immune responses after tularaemia immunisation
Authors: Salerno‐Gonçalves Rosangela , Chen Wilbur H , Mulligan Mark J , Frey Sharon E , Stapleton Jack T , Keitel Wendy A , Bailey Jason , Sendra Eli , Hill Heather , Johnson Robert A , Sztein Marcelo B 
Published: January 2021

AbstractObjectivesFrancisella tularensis, the causative agent of tularaemia, is an exceptionally infectious bacterium, potentially fatal for humans if left untreated and with the potential to be developed as a bioweapon. Both natural infection and live‐attenuated vaccine strain (LVS) confer good protection against tularaemia. LVS vaccination is traditionally administered by scarification, and the formation of a cutaneous reaction or take at the vaccination site is recognised as a clinical correlate of protection. Although previous studies have suggested that high antibody titres following vaccination might serve as a useful surrogate marker, the immunological correlates of protection remain unknown.MethodsWe investigated the host T‐cell‐mediated immune (T‐CMI) responses elicited following immunisation with LVS vaccine formulated by the DynPort Vaccine Company (DVC‐LVS) or the United States Army Medical Research Institute of Infectious Diseases (USAMRIID‐LVS). We compared T‐CMI responses prompted by these vaccines and correlated them with take size.ResultsWe found that both LVS vaccines elicited similar T‐CMI responses. Interestingly, take size associated with the T cells’ ability to proliferate, secrete IFN‐γ and mobilise degranulation, suggesting that these responses play an essential role in tularaemia protection.ConclusionsThese results renew the appreciation for vaccination through the scarification as a prime route of inoculation to target pathogens driving specific T‐CMI responses and provide further evidence that T‐CMI plays a role in protection from tularaemia.
Neural mechanisms of reward processing in adolescent irritability
Authors: Kryza‐Lacombe Maria , Hernandez Brianna , Owen Cassidy , Reynolds Richard C. , Wakschlag Lauren S. , Dougherty Lea R. , Wiggins Jillian L. 
Published: January 2021

AbstractIrritability is impairing and prevalent across pediatric psychiatric disorders and typical development, yet its neural mechanisms are largely unknown. This study evaluated the relation between adolescent irritability and reward‐related brain function as a candidate neural mechanism. Adolescents from intervention‐seeking families in the community (N = 52; mean age = 13.80, SD = 1.94) completed a monetary incentive delay task to assess reward anticipation and feedback (reward receipt and omission) during fMRI acquisition. Whole‐brain analyses, controlling for age, examined brain activation and striatal and amygdala connectivity in relation to irritability. Irritability was measured using the parent‐ and youth‐reported Affective Reactivity Index. Irritability was associated with altered reward processing‐related activation and connectivity in multiple networks during reward anticipation and feedback, including increased striatal activation and altered ventral striatum connectivity with prefrontal areas. Our findings suggest that irritability is associated with altered neural patterns during reward processing and that aberrant prefrontal cortex‐mediated top‐down control may be related to irritability. These findings inform our understanding of the etiology of youth irritability and the development of mechanism‐based interventions.
Mud Dauber Nests as Sources of Spiders in Mercury Monitoring Studies
Authors: Hannappel Madeline P. , Chumchal Matthew M. , Drenner Ray W. , Kennedy Jim , Barst Benjamin D. , Castellini J. Margaret 
Published: January 2021

Abstract
Identifying ecosystems where biota may be contaminated with hazardous levels of methylmercury (MeHg) is a challenge. One widely used approach for determining site-specific MeHg contamination is to monitor MeHg contamination in sentinel species. Terrestrial shoreline spiders that consume emergent aquatic insects (e.g., midges and mayflies) have been proposed as sentinels of MeHg contamination of aquatic ecosystems. The purpose of the present study was to determine whether a novel sampling technique, collection of spiders from nests of mud dauber wasps (Sphecidae), would be an efficient method for capturing MeHg-contaminated shoreline spiders for use as sentinels in ecological risk assessments. Mud dauber nests were collected near the Clear Fork of the Trinity River in Fort Worth, Texas (USA) on 3 dates from 4 human-made structures. Nests contained 627 unconsumed spiders from 5 families: Araneidae, Salticidae, Thomisidae, Oxyopidae, and Theridiidae. Methylmercury concentrations ranged from 12.2 to 56.3 ng/g wet weight in Thomisidae and Araenidae, respectively. Methylmercury concentrations of the spiders were generally low relative to risk thresholds for adult birds, but a few families of spiders could pose a risk to nestlings. Although mud dauber nests have been recognized as a source of spiders for biodiversity studies, the present study is the first to demonstrate the potential use of spiders collected from mud dauber nests for ecotoxicology studies. Environ Toxicol Chem 2021;40:1335–1340. © 2021 SETAC
OK, Boomer, MD: The Rights of Aging Physicians and the Health of Our Communities
Authors: Powell Tia 
Published: January 2021

AbstractHow do we balance the rights of aging physicians against the right of the public to competent health care? This version of a classic public health ethics dilemma is here now and likely to increase as the population ages. Peer review has long been the standard mechanism for assessing physician competence, but it is subjective and too easily subverted. New options are needed, both in medicine and throughout the professions, but they are challenging to implement. Physicians have an ethical obligation to protect the health of the public by acknowledging, assessing, and addressing the cognitive effects of aging on medical competence.
Realizing Present and Future Promise of DIY Biology and Medicine through a Trust Architecture
Authors: Rasmussen Lisa M. , Guerrini Christi J. , Kuiken Todd , Nebeker Camille , Pearlman Alex , Ware Sarah B. , Wexler Anna , Zettler Patricia J. 
Published: January 2021

AbstractThe speed and scale of the COVID‐19 pandemic has highlighted the limits of current health systems and the potential promise of non‐establishment research such as “DIY” research. We consider one example of how DIY research is responding to the pandemic, discuss the challenges faced by DIY research more generally, and suggest that a “trust architecture” should be developed now to contribute to successful future DIY efforts.
Multiparametric and multilevel characterization of morphological alterations in patients with transient ischemic attack
Authors: Lv Yating , Wei Wei , Han Xiujie , Song Yulin , Han Yu , Zhou Chengshu , Zhou Dan , Zhang Fuding , Wu Xiaoyan , Liu Jinling , Zhao Lijuan , Zhang Cairong , Wang Ningkai , Wang Jinhui 
Published: January 2021

AbstractTransient ischemic attack (TIA), an important risk factor for stroke, is associated with widespread disruptions of functional brain architecture. However, TIA‐related structural alterations are not well established. By analyzing structural MRI data from 50 TIA patients versus 40 healthy controls (HCs), here we systematically investigated TIA‐related morphological alterations in multiple cortical surface‐based indices (cortical thickness [CT], fractal dimension [FD], gyrification index [GI], and sulcal depth [SD]) at multiple levels (local topography, interregional connectivity and whole‐brain network topology). For the observed alterations, their associations with clinical risk factors and abilities as diagnostic and prognostic biomarkers were further examined. We found that compared with the HCs, the TIA patients showed widespread morphological alterations and the alterations depended on choices of morphological index and analytical level. Specifically, the patients exhibited: (a) regional CT decreases in the transverse temporal gyrus and lateral sulcus; (b) impaired FD‐ and GI‐based connectivity mainly involving visual, somatomotor and ventral attention networks and interhemispheric connections; and (c) altered GI‐based whole‐brain network efficiency and decreased FD‐based nodal centrality in the middle frontal gyrus. Moreover, the impaired morphological connectivity showed high sensitivities and specificities for distinguishing the patients from HCs. Altogether, these findings demonstrate the emergence of morphological index‐dependent and analytical level‐specific alterations in TIA, which provide novel insights into neurobiological mechanisms underlying TIA and may serve as potential biomarkers to help diagnosis of the disease. Meanwhile, our findings highlight the necessity of using multiparametric and multilevel approaches for a complete mapping of cerebral morphology in health and disease.
Mutational Signature Analysis Reveals Widespread Contribution of Pyrrolizidine Alkaloid Exposure to Human Liver Cancer
Authors: He Yisheng , Shi Mai , Wu Xu , Ma Jiang , Ng Kevin Tak‐Pan , Xia Qingsu , Zhu Lin , Fu Peter Pi‐Cheng , Man Kwan , Tsui Stephen Kwok‐Wing , Lin Ge 
Published: January 2021

Background and Aims
Mutational signature analyses are an effective tool in identifying cancer etiology. Humans are frequently exposed to pyrrolizidine alkaloids (PAs), the most common carcinogenic phytotoxins widely distributed in herbal remedies and foods. However, due to the lack of human epidemiological data, PAs are classified as group II hepatocarcinogens by the World Health Organization. This study identified a PA mutational signature as the biomarker to investigate the association of PA exposure with human liver cancer.

Approach and Results
Pyrrole‐protein adducts (PPAs), the PA exposure biomarker, were measured and found in 32% of surgically resected specimens from 34 patients with liver cancer in Hong Kong. Next, we delineated the mode of mutagenic and tumorigenic actions of retrorsine, a representative PA, in mice and human hepatocytes (HepaRG). Retrorsine induced DNA adduction, DNA damage, and activation of tumorigenic hepatic progenitor cells, which initiated hepatocarcinogenesis. PA mutational signature, as the unique molecular fingerprint of PA‐induced mutation, was derived from exome mutations in retrorsine‐exposed mice and HepaRG cells. Notably, PA mutational signature was validated in genomes of patients with PPA‐positive liver cancer but not patients with PPA‐negative liver cancer, confirming the specificity of this biomarker in revealing PA‐associated liver cancers. Furthermore, we examined the established PA mutational signature in 1,513 liver cancer genomes and found that PA‐associated liver cancers were potentially prevalent in Asia (Mainland China [48%], Hong Kong [44%], Japan [22%], South Korea [6%], Southeast Asia [25%]) but minor in Western countries (North America [3%] and Europe [5%]).

Conclusions
This study provides a clinical indication of PA‐associated liver cancer. We discovered an unexpectedly extensive implication of PA exposure in patients with liver cancer, laying the scientific basis for precautionary approaches and prevention of PA‐associated human liver cancers.
A thematic analysis of men’s help‐seeking on an Australian health helpline
Authors: Lopriore Stefanie , Turnbull Deborah , Martin Sean A. 
Published: January 2021

AbstractAims and objectivesThe current study aimed to qualitatively explore men's help‐seeking behaviours by analysing male callers’ talk on an Australian health helpline. Analysis focused on identifying the ways in which men positioned themselves as help‐seekers and the extent to which help‐seeking behaviours deviated or conformed to the narrative commonly reported on in men's health literature which, generally speaking, positions men as reluctant help‐seekers and health service users.MethodsThe present study utilised naturally occurring instances of men's help‐seeking during 196 calls to the helpline, Healthdirect Australia. Thematic analysis was used to explore recurrent themes in help‐seeking interactions.FindingsThe analysis yielded three broad themes, which were formulated as caller archetypes. These themes included the ‘reluctant caller’, the ‘unwell patient’ and the ‘concerned carer’, as well as a number of associated sub‐themes within these broad categories.ConclusionsThe findings demonstrated that male callers sought help in a variety of different ways, rather than prescribing to a homogenous pattern of help‐seeking. However, it was acknowledged that some data did align with help‐seeking behaviour which indicated men's reluctance to engage with their own health.So what?The present study contributes to men's health promotion by identifying the various social devices used by men to facilitate help‐seeking. The findings highlight the changing and flexible landscape of contemporary masculinity and its impact on health engagement. Recognising the versatility in men's health behaviour is important for ensuring that men have positive experiences during healthcare encounters which, in turn, may facilitate future health service uptake and engagement.
Soret, viscous dissipation, and thermal radiation effects on MHD free convective flow of Williamson liquid with variable viscosity and thermal conductivity
Authors: Onwubuoya Cletus , Dada Moses S. 
Published: January 2021

AbstractThe flow model of heat and mass transport of a Williamson liquid through a porous stretching sheet with radiation, viscous dissipation, Soret effect, and chemical reaction has been explored. The motion starts from the slot to the free stream. The present study is unique, because it examines the flow of a Williamson fluid under the influence of variable viscosity and thermal conductivity. The Williamson fluid term as added to the momentum and energy equation is considered in a nonlinear form as compared with other studies in literature. The flow model is a set of coupled highly nonlinear partial differential equations that are simplified and lead to coupled nonlinear total differential equations by employing sufficient similarity variables. The simplified equations are later solved by utilizing the spectral homotopy analysis method. Our experiment shows that the injected variable viscosity, together with thermal conductivity, has a great impact on the fluid profiles. An increase in the Williamson parameter (β) leads to a decrease in the thickness of the hydrodynamic thermal layer. Our numerical calculations were compared with earlier published work, and they were discovered to be correct.
Physical Determinants of Vitamin D Photosynthesis: A Review
Authors: Neville Jonathan J , Palmieri Tommaso , Young Antony R 
Published: January 2021

ABSTRACTVitamin D synthesis by exposure of skin to solar ultraviolet radiation (UVR) provides the majority of this hormone that is essential for bone development and maintenance but may be important for many other health outcomes. This process, which is the only well‐established benefit of solar UVR exposure, depends on many factors including genetics, age, health, and behavior. However, the most important factor is the quantity and quality of UVR reaching the skin. Vitamin D synthesis specifically requires ultraviolet B (UVB) radiation that is the minority component (<5%) of solar UVR. This waveband is also the most important for the adverse effects of solar exposure. The most obvious of which is sunburn (erythema), but UVB is also the main cause of DNA damage to the skin that is a prerequisite for most skin cancers. UVB at the Earth's surface depends on many physical and temporal factors such as latitude, altitude, season, and weather. Personal, cultural, and behavioral factors are also important. These include skin melanin, clothing, body surface area exposed, holiday habits, and sunscreen use. There is considerable disagreement in the literature about the role of some of these factors, possibly because some studies have been done by researchers with little understanding of photobiology. It can be argued that vitamin D supplementation obviates the need for solar exposure, but many studies have shown little benefit from this approach for a wide range of health outcomes. There is also increasing evidence that such exposure offers health benefits independently of vitamin D: the most important of which is blood‐pressure reduction. In any case, public health advice must optimize risk versus benefit for solar exposure. It is fortunate that the individual UVB doses necessary for maintaining optimal vitamin D status are lower than those for sunburn, irrespective of skin melanin. © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.
EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease
Authors: Vaught David B , Merkel Alyssa R , Lynch Conor C , Edwards James , Tantawy Mohammed Noor , Hilliard Timothy , Wang Shan , Peterson Todd , Johnson Rachelle W , Sterling Julie A , Brantley‐Sieders Dana 
Published: January 2021

ABSTRACTEphA2 receptor tyrosine kinase (RTK) is highly expressed in breast tumor cells across multiple molecular subtypes and correlates with poor patient prognosis. In this study, the potential role of EphA2 in this clinically relevant phenomenon is investigated as metastasis of breast cancer to bone is a major cause of morbidity and mortality in patients. It was found that the EphA2 function in breast cancer cells promotes osteoclast activation and the development of osteolytic bone disease. Blocking EphA2 function molecularly and pharmacologically in breast tumors reduced the number and size of bone lesions and the degree of osteolytic disease in intratibial and intracardiac mouse models, which correlated with a significant decrease in the number of osteoclasts at the tumor–bone interface. EphA2 loss of function in tumor cells impaired osteoclast progenitor differentiation in coculture, which is mediated, at least in part, by reduced expression of IL‐6. EPHA2 transcript levels are enriched in human breast cancer bone metastatic lesions relative to visceral metastatic sites; EphA2 protein expression was detected in breast tumor cells in bone metastases in patient samples, supporting the clinical relevance of the study's findings. These data provide a strong rationale for the development and application of molecularly targeted therapies against EphA2 for the treatment of breast cancer bone metastatic disease. © 2021 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.
One more time with less jargon: An introduction to “Rupture Repair in Practice”
Authors: Muran J. Christopher , Eubanks Catherine F. , Samstag Lisa Wallner 
Published: January 2021

AbstractIn this introduction to this issue on Rupture–Repair in Practice, we present our understanding of alliance ruptures using common language to appeal to all theoretical orientations. Specifically, we define withdrawal movements away from another or oneself (efforts towards isolation or appeasement) and confrontation movements against another (efforts towards aggression or control). In addition to these interpersonal markers, we suggest that therapist emotional experiences can be considered as intrapersonal markers indicating rupture. We emphasize understanding ruptures as relational phenomena. Then we present various pathways toward rupture–repair, highlighting renegotiation of therapy tasks or goals and exploration of patient and therapist contributions and needs. We explain how these paths can be understood as critical change processes that can transform obstacles in treatment into opportunities. We finish with some mention of our alliance‐focused training for self‐development. This issue represents an important step towards demonstrating the transtheoretical and practical potential of rupture–repair.
Prevalence and factors associated with poor performance in the 5‐chair stand test: findings from the Cognitive Function and Ageing Study II and proposed Newcastle protocol for use in the assessment of sarcopenia
Authors: Dodds Richard Matthew , Murray James C. , Granic Antoneta , Hurst Christopher , Uwimpuhwe Germaine , Richardson Sarah , Brayne Carol , Matthews Fiona E. , Sayer Avan A. ,   MRC CFAS
Published: January 2021

AbstractBackgroundPoor performance in the 5‐chair stand test (5‐CST) indicates reduced lower limb muscle strength. The 5‐CST has been recommended for use in the initial assessment of sarcopenia, the accelerated loss of muscle strength and mass. In order to facilitate the use of the 5‐CST in sarcopenia assessment, our aims were to (i) describe the prevalence and factors associated with poor performance in the 5‐CST, (ii) examine the relationship between the 5‐CST and gait speed, and (iii) propose a protocol for using the 5‐CST.MethodsThe population‐based study Cognitive Function and Ageing Study II recruited people aged 65 years and over from defined geographical localities in Cambridgeshire, Newcastle, and Nottingham. The study collected data for assessment of functional ability during home visits, including the 5‐CST and gait speed. We used multinomial logistic regression to assess the associations between factors including the SARC‐F questionnaire and the category of 5‐CST performance: fast (<12 s), intermediate (12–15 s), slow (>15 s), or unable, with slow/unable classed as poor performance. We reviewed previous studies on the protocol used to carry out the 5‐CST.ResultsA total of 7190 participants aged 65+ from the three diverse localities of Cognitive Function and Ageing Study II were included (54.1% female). The proportion of those with poor performance in the 5‐CST increased with age, from 34.3% at age 65–69 to 89.7% at age 90+. Factors independently associated with poor performance included positive responses to the SARC‐F questionnaire, physical inactivity, depression, impaired cognition, and multimorbidity (all P < 0.005). Most people with poor performance also had slow gait speed (57.8%) or were unable to complete the gait speed test (18.4%). We found variation in the 5‐CST protocol used, for example, timing until a participant stood up for the fifth time or until they sat down afterwards.ConclusionsPoor performance in the 5‐CST is increasingly common with age and is associated with a cluster of other factors that characterize risk for poor ageing such as physical inactivity, impaired cognition, and multimorbidity. We recommend a low threshold for performing the 5‐CST in clinical settings and provide a protocol for its use.