bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on Brain Tumors in Mexico: A Bibliometric Analysis from the Latin America
Authors: Rubio Carmen , Tena Martha , Rojas Daniel , Sotelo Julio , Lee Angle 
Published: November 2021

Abstract
Background: Bibliometrics is a completive method of research and analyses useful to understand the collective contributions of a given scientific community. The detail of the quantity (productivity) and impact as a surrogate marker of quality can shed light on what we have done (number of articles) and how we impact others (citations). It also shows the direction the community can take for further research, guided by its shortcomings and successes. The publications of Latin American medical institutions on brain neoplasms have never been studied.Objective: Analyze a clinical and experimental approach, identifying core journals, type of article, increase of published material with time, number of citations. Additionally, we identified the most researched topics involved in brain tumor literature.Material and methods: We harvested the articles published by at least one author form the Instituto Nacional de Neurología y Neurocirugía form Mexico since its inception in 1964 to 2020. Key bibliometric parameters, as journal of publication, type of paper, number of articles and citations were recorded. Results: In cerebral neoplasms, our institution produced 291 articles (clinical versus experimental: 227 vs. 64). With a modest productivity before 2000, the production had increased 8-fold by 2019. The main topic is glioblastoma, also with the highest number of citations. Researchers prefer to conduct original investigations rather than to subject reviews. Most papers were published in Archivos de Neurociencias (institutional journal produced in Spanish), most papers published in English were in Journal of Neuro-Oncology. Discussion: Productivity had an encouraging growth in the last decade, but more emphasis should be given to target international journals, which offer a high number of readers and citations. Strategies to reach these goals have to be found and should be implemented.Conclusion: Research on brain tumors in Mexico has recently showed buoyancy and we should profit from this inertia to give a definitive boost to it, which might benefit authors and institutional prestige. But mainly, with a more robust research, we could find better solutions for our patients, applicable in the national and international context.
The impact of linkage disequilibrium and epistasis in the studies of inheritance based on Hayman’s diallel and generation mean analysis
Authors: Viana José Marcelo Soriano 
Published: November 2021

Abstract
This simulation-based study assessed the impact of linkage disequilibrium (LD) and epistasis on Hayman’s diallel and generation mean analysis, assuming hundreds of genes, variable degree of dominance, and seven types of digenic epistasis. The diallel parents were 15 doubled-haploid lines from a high LD population. The generation mean analysis was based on seven generations, assuming association. Under low LD and no epistasis, the diallel analysis provided confident results about the inheritance of the quantitative trait and high correlation between number of recessive genes and Wr + Vr, but biased estimates of the dominance components and genetic parameters. The additional consequences of high LD under no epistasis were rejection of the additive-dominance model assuming high heritability and lower correlation. Assuming 100% of epistatic genes, for four epistasis types there was evidence of inadequacy of the additive-dominance model. Assuming 30% of epistatic genes, there was a tendency for accepting the additive-dominance model for low heritability traits and for rejecting for high heritability traits. Linkage and epistasis affects the estimates of the genetic components of the generation means. Even assuming 100% of interacting genes, for most epistasis types there was no statistical evidence of epistasis. Assuming positive partial dominance, the signs of the epistatic components do not allow discriminate complementary, recessive, dominant and recessive, duplicate genes with cumulative effects, and non-epistatic genic interaction. Negative epistatic components evidence dominant epistasis. When the additive x additive and dominance x dominance components are positive and the additive x dominance component is negative, there is duplicate epistasis.
Conductometric assisted ZnO and polypyrole composite based NH3 gas sensor
Authors: GAHLOT AJAY PRATAP SINGH , Paliwal Ayushi , Kapoor Avinashi 
Published: November 2021

Abstract
Metal oxides are excellent choices as base materials in emerging technologies in the field of Gas Sensors. In this work, an effort is made to prepare the ZnO and polypyrrole composite thin film using sol-gel technique. Nanocomposite thins film was prepared using spin coating on glass substrates for ammonia gas sensing. ZnO and polypyrrole sols were prepared using sol-gel technique and thin films were prepared by using spin coater. The nanocomposite thin films were prepared by varying the percentage composition of ZnO and Polypyrrole. Structural, optical and morphological properties of the prepared films were done using XRD, UV-Visible and SEM studies. The films were separately prepared on Inter-Digital Transducers for gas sensing applications. Gas sensing response characteristics of the prepared sensor were performed using laboratory. The sensing response of the prepared films is observed and found to be maximum (~33) for the 70%PPy+30%ZnO film at a relatively low operating temperature of about 150°C.
Day 3 Embryo Fragmentation Is Associated With Singleton Birthweight Following Fresh Single Blastocyst Transfer：A Retrospective Study
Authors: Cai jiali , Liu Lanlan , Chen Jinghua , Liu Zhenfang , Wang Wenjie , Jiang Xiaoming , Chen Haixiao , Ren Jianzhi 
Published: November 2021

Abstract
Background

Previous studies arguably associated poor embryo morphology with low birthweight in singletons following single embryo transfer. However, the association between specific morphological features on the cleavage stage and birthweight is still less known. The purpose of the study was to investigate whether embryo morphological features at the cleavage stage affect birthweight following blastocyst transfer
Methods

The single-center, retrospective cohort study included 4226 singletons derived from fresh single cleavage stage embryo transfer (ET, n=1185), fresh single blastocyst transfer (BT, n=787), or frozen-thawed single blastocyst transfer (FBT, n=2254) between 2016 and 2019. The morphological parameters including early cleavage, day 3 fragmentation, symmetry, blastomere number, and blastocyst morphology were associated with neonatal birthweight and z-score in multivariate regression models. Models were adjusted for maternal age, BMI, parity, peak estradiol level, endometrial thickness, insemination protocol, female etiologies, order of transfer, mode of delivery, and year of treatment.
Results

Adjusted for confounders, fragmentation was the only morphology feature associated with birthweight and z-score, while early cleavage, symmetry, blastomere number and blastocyst morphology were not. Fragmentation increased the birthweight in both ET group (115.4g, 95% CI: 26.6 to 204.2) and BT group (168.8g, 95%CI: 48.8 to 288.8), but not in FBT group (7.47g, 95%CI: -46.4 to 61.3). The associations of birthweight and morphological parameters were confirmed in analyses for z-score. Adjusted odds of large for gestational age and high birthweight were also significantly greater in singletons following the transfer of fragmented embryos in BT group (OR 3, 95% CI: 1.2 to 7.51, OR 3.65, 95% CI: 1.33 to 10, respectively). The presence of fragmentation at the cleavage stage also affected the association between blastocyst morphology and birthweight. Inner cell mass grades were negatively associated with birthweight in blastocysts with day 3 fragmentation but not in blastocysts without.
Conclusions

Birthweight following blastocyst transfer is positively associated with fragmentation at the cleavage stage. The data did not support the argument that transferring a poor-looking embryo may increase the risks of low birthweight. However, concerns for LGA infants still remain.
Pregnancy Risk Perception and Associated Factors Among Pregnant Women Attending Antenatal Care at Health Centers in Jabitehnan District, Amhara, Northwest Ethiopia
Authors: Alemu Demeke Andebet , Zegeye Ambaye Minayehu Minayehu , Dessie Wale Kumlachew , Zeleke Liknaw Bewket , Belay Dawit Misganaw 
Published: November 2021

Abstract
Background: Pregnancy risk perception affects a pregnant woman’s decision about health care services use such as prenatal care, place of birth, choices about medical interventions, adherence to medical procedures, and recommendations. Methods: An institutional-based cross-sectional study was conducted among 424 mothers attending ANC at health centers in the Jabitenhan district from April 1 to 30, 2021. Data was collected through a face-to-face interview using a structured questionnaire which was developed according to the health belief model. The logistic regression model was used using adjusted odds ratio with 95% CI and p value <0.05Result: 424 pregnant women were interviewed of which nearly half of the respondents (48%) had good pregnancy risk perception. Women who had a history of obstetric complication (AOR:95%CI = 3.44:1.73,6.83), those who know at least one pregnancy danger sign (AOR:95%CI =5.22;2.46,11.07), pregnant women who had a bad obstetric history (AOR:95%CI = 2.23:1.13,4.41) and knowing women who died due to pregnancy-related complications (AOR:95%CI =2.85:1.45,5.60) were more likely to have good perception towards pregnancy risk as compared to their counterparts.Conclusion: Obstetric complications, awareness of pregnancy danger signs, bad obstetric history, and knowing women who died due to pregnancy-related complications were found to be significantly associated with pregnancy risk perception.
High-Speed Optical Resolution Photoacoustic Microscopy With a MEMS Scanner: Novel and Simple Distortion Correction Method
Authors: Shintate Ryo , Ishii Takuro , Ahn Joongho , Kim Jin Young , Kim Chulhong , Saijo Yoshifumi 
Published: November 2021

Abstract
Optical resolution photoacoustic microscopy (OR-PAM) is a remarkable biomedical imaging tool that can selectively visualize microtissues with optical-dependent high resolution. However, traditional OR-PAM using mechanical stages provides slow imaging speed, making biological interpretation of in-vivo tissue difficult. Here, we developed a high-speed OR-PAM using a recently commercialized MEMS mirror. This system (MEMS-OR-PAM) consisted of a 1-axis MEMS mirror and a mechanical stage. Furthermore, this study proposed a novel calibration method that quickly removes the spatial distortion caused by fast MEMS scanning. The proposed calibration method needs to run imaging sequence only once using a ruler target and it can easily correct distortions caused by both the scan geometry of the MEMS mirror and its nonlinear motion. The combination of the MEMS-OR-PAM and the distortion correction method was verified by three experiments.; 1) Leaf skeleton phantom imaging to test the distortion correction efficacy.; 2) Spatial resolution and depth of focus (DOF) measurement for the system performance.; 3) In-Vivo finger capillaries imaging to verify their biomedical use. The results showed that the combination could achieve a high-speed (32 sec in 2 mm×4 mm) and high-lateral resolution (~6 µm) imaging capability and precisely visualize the circulating structure of the finger capillaries.
Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during algal blooms
Authors: VINCENT Flora , Gralka Matti , Schleyer Guy , Schatz Daniella , Cabrera-Brufau Miguel , Kuhlisch Constanze , Sichert Andreas , Vidal-Melgosa Silvia , Mayers Kyle , Barak-Gavish Noa , Flores J. Michel , Masdeu-Navarro Marta , Egge Jorun , Larsen Aud , Heheman Jan-Hendrick , Marrase Celia , Simo Rafel , Cordero Otto , Vardi Assaf 
Published: November 2021

Abstract
Algal blooms are hotspots of marine primary production and play central roles in microbial ecology and global nutrient cycling. When blooms collapse, organic carbon is transferred to higher trophic levels, microbial respiration or sinking in proportions that depend on the dominant mortality agent. Viral infection can lead to bloom termination, but its impact on the fate of carbon remains an open question. Here, we characterized the consequences of viral infection on the microbiome composition and biogeochemical landscape of marine ecosystems by conducting a large-scale mesocosm experiment. Moniroting of seven induced coccolithophore blooms, which showed different degrees of viral infection, revealed that only high levels of viral infection caused significant shifts in the composition of free-living bacterial and eukaryotic assemblages. Intriguingly, viral infection favored the growth of eukaryotic heterotrophs (thraustochytrids) over bacteria as potential recyclers of organic matter. By combining modeling and quantification of active viral infection at a single-cell resolution, we estimate that viral infection can increase per-cell rates of extracellular carbon release by 2-4.5 fold. This happened via production of acidic polysaccharides and particulate inorganic carbon, two major contributors to carbon sinking into the deep ocean. These results reveal the impact of viral infection on the fate of carbon through microbial recyclers of organic matter in large-scale coccolithophore blooms.
Identification of Candidate Genes Associated with Bacterial and Viral Infections in Wild Boars Hunted in Tuscany (Italy)
Authors: Fabbri Maria Chiara , Crovetti Alessandro , Tinacci Lara , Bertelloni Fabrizio , Armani Andrea , Mazzei Maurizio , Fratini Filippo , Bozzi Riccardo , Cecchi Francesca 
Published: November 2021

Abstract
Wild boar (Sus scrofa L.) is one of the large mammals most spread worldwide, highly adaptable, and its population has rapidly increased in many areas in Europe. Central Italy, as well as Tuscany, is an area particularly suitable for wild boar. Wild boars are potential hosts for different etiological agents, such as Brucella spp., Leptospira spp. and Pseudorabies virus and can contribute to maintain and/or to disseminate some bacterial or viral pathogens to humans and domestic animals, above all-in free-range farms. In order to identify hypothetical genomic regions associated with these infection diseases, 96 samples of wild boars hunted in Tuscany during the 2018–2019 and 2019-2020 hunting seasons were considered. Diagnosis was achieved by serological tests and 42 Pseudorabies, 31 Leptospira and 15 Brucella positive animals were identified. All animals were genotyped with Geneseek Genomic Profiler Porcine HD (70k) and a genome-wide scan was then performed. Significant markers were highlighted for Pseudorabies (two SNPs), Brucella (seven SNPs), and Leptospira (four SNPs) and they were located within, or nearby, 29 annotated genes on chromosome 6, 9, 12, 13, 14 and 18. Eight genes are implicated in viral (SEC14L1, JMJD6, SRSF2, TMPRSS2, MX1, MX2) or bacterial (COL8A1, SPIRE1) infections, seven genes (MFSD11, METTL23, CTTNBP2, BACE2, IMPA2, MPPE1 and GNAL) are involved in mental disorders and one gene (MGAT5B) is related to the Golgi complex. Results presented here provide interesting starting points for future research, validation studies and fine mapping of candidate genes involved in bacterial and viral infections in wild boar.
Histopathological and Cytogenetic Analysis of Epidural Adipose Tissue in Symptomatic Lumbar Epidural Lipomatosis
Authors: Yasuda Taketoshi , Suzuki Kayo , Seki Shoji , Watanabe Kenta , Makino Hiroto , Kanamori Masahiko , Kawaguchi Yoshiharu 
Published: November 2021

Abstract
Lumbar epidural lipomatosis (LEL) is characterized by the abnormal accumulation of unencapsulated adipose tissue in the spinal epidural space. Symptomatic LEL occurs in middle aged and elderly patients, and it is most often seen in males. The purpose of this study was to elucidate the histopathological and cytogenetic characteristics of the epidural lipomatosis in patients with symptomatic LEL. Six patients undergoing decompressive spinal surgery (mean age, 69.4 years) were enrolled between 2013 and 2017. Three cases were steroid-induced and 3 cases were diagnosed as idiopathic LEL. We compared the differences in histological morphology between the subcutaneous fat tissue and epidural fat tissue in each patient. We also analyzed the karyotype of resected epidural lipomatous tissue using the G-band method. The epidural adipocytes were histologically more irregular and smaller compared with the subcutaneous adipocytes in all cases. The mean size of subcutaneous adipocytes and epidural adipocytes was 5,485.6 ± 2,584.1 µm2 and 2,705.4 ± 1,069.1 µm2, respectively. In cytogenetic analysis of the epidural adipocytes, loss of the Y chromosome (LOY) was found in all 6 cases. The mechanisms related to the development of LEL are not well understood. In this study, the size of the lipid component of epidural adipocytes was significantly smaller than that of subcutaneous adipocytes in LEL. Cytogenetically, LOY was frequently recognized. Although this may be an effect rather than a cause, LOY may be related to morphologic changes in and proliferation of adipocytes in LEL. LOY might partially contribute to the pathological mechanism or expression of LEL.
Transcriptome Analysis of EnJSRV-Env on the Inhibition of Cell Proliferation
Authors: Wang Xiaojuan , Zhang Liang , Liu Shuying 
Published: November 2021

Abstract
Background

Endogenous retroviruses (ERVs) exert important biological roles, such as mammalian placental development and suppression of the infection of exogenous retrovirus. In addition, ERVs could also inhibit the proliferation of cell. The envelope-protein (Env) of endogenous Jaagsiekte sheep retroviruses (enJSRVs) possesses fusogenic activity, which promoted the formation of nonproliferative multinucleated syncytiotrophoblasts.
Methods

The proliferation of HeLa cells was detected by MTT. Six samples were extracted for RNA-seq transcriptome analysis. Quantitative real-time PCR (qRT-PCR) and western blotting were employed for the validation of interested target.
Results

enJSRV-Env transfection inhibited the proliferation ability of Hela cells and 170 differentially expressed genes (DEGs) were obtained by RNA-seq analysis. Among these, 5 DEGs (BHLHE41, CCN1, DLX2, DUSP6 and SH2D5) were validated by qRT-PCR, which closely related with proliferation. Western blotting analysis showed that the expression of DUSP6 and p-ERK1/2 was decreased, which suggested that ERK1/2 signaling pathway may be involved in enJSRV-Env transfection.
Conclusions

enJSRV-Env transfection inhibited the proliferation of Hela cells, probably via DUSP6 and ERK1/2 signaling pathway.
Endothelial Cells Immune-Related Genes Risk Signature Correlated With Tumor Immune Microenvironment Predicts Therapeutic Response and Prognosis in Glioblastoma
Authors: Xie Qijun , Zhang Yuwei , Huang Wu , Wang Haoran , Liu Fang 
Published: November 2021

Abstract
Glioblastoma (GBM) is the most common and aggressive primary tumor of the central nervous system with high recurrence and extremely poor prognosis. Multiple recent studies have indicated a pivotal correlation between GBM prognosis and immune-related risk signature. Nevertheless, the potential value of endothelial cells (ECs) immune-related genes (EIRGs) in prognosis, immune infiltration, and their correlation with therapeutic response to immunotherapy and TMZ chemotherapy remain obscure, especially in GBM. Here, we screened out 11 EIRGs after intersecting the identified 59 GBM ECs related prognostic genes and the identified 438 immune-related prognostic genes. A prognostic-related 6-EIRGs signature was established through univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) Cox regression analysis, and patients in the high-risk group were significantly worse overall survival (OS) compared to those in the low-risk group. Additionally, univariate and multivariate Cox regression analysis confirmed that risk score was an independent predictor of OS in patients with GBM. The nomogram which comprised age, gender, IDH mutation status, radiation therapy, and risk score yielded a strong predictive ability of 0.5-, 1-, and 2-year OS for GBM patients. Our results demonstrate that the EIRGs signature, which is associated with immune cell infiltration, may play a regulatory role in the immunobiological process of TIME. Prognostic-related 6-EIRGs signature is a promising classification index for predicting the drug sensitivity to immunotherapy and TMZ chemotherapy, suggesting that EIRGs signature may serve as a biomarker to stratify patients who will benefit from immunotherapy and chemotherapy.
Dysregulation of Transcription Factor EB Contributes to Cell Proliferation, Metastasis and Stemness in Breast Cancer
Authors: Fu Ningwei , Fan Ning , Luo Wenchao , Lv Lijia , Li Jing , Mao Jun , Li Feifei , Wang Xujiao , Zhang Xin , Xu Fei , Li Yan , Ma Wei 
Published: November 2021

Abstract
Purpose: TFEB is a key regulator of autophagy-lysosomal biogenesis pathways, while its dysregulation is highly prevalent in various human cancers, but the specific contribution to breast cancer remains poorly understood. The main purpose of this study is to explore the role of TFEB in breast cancer proliferation, metastasis and maintaining breast cancer stem cells (BCSCs) traits, thus uncovering its underlying mechanism.Methods: Bioinformatics, western blotting and immunohistochemical staining were applied to analyze the expression of TFEB in breast cancer. Stable down-regulation TFEB cells were established in MCF-7 and MDA-MB-231 breast cancer cell lines. MTT, clone formation, wound healing, transwell and 3D tumor invasion assays were used to evaluate the proliferation, migration and invasion ability of breast cancer cells. Mammosphere formation, immunocytochemical (ICC) staining were used to detect the effect of down-regulating TFEB on breast cancer stem cells. Results: we demonstrated that higher expression of TFEB was found in breast cancer. TFEB depletion had inhibitory effects on cellular proliferation, migration and invasion of breast cancer cells. Moreover, knockdown TFEB decreased mammosphere formation ability of BCSCs and expression of cancer stem cell markers. Autophagy-lysosomal related proteins were decreased by down regulation of TFEB. Conclusion: we uncovered a critical role of TFEB in breast cancer proliferation and metastasis, and BCSCs self-renewal and stemness. The underlying mechanisms involve in maintaining BCSCs traits, and dysregulating lysosome functions.