bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis Derives a Novel Prognostic 7-mRNA Signature in Early-Stage Triple-Negative Breast Cancer
Authors: Yang Yun-Song , Ren Yi-Xing , Hao Shuang , Xu Xiao-En , Jin Xi , Jiang Yi-Zhou , Shao Zhi-Ming 
Published: April 2021

Abstract
Background: Triple-negative breast cancer (TNBC) is a highly heterogeneous disease and patients with early-stage TNBCs have distinct likelihood of distant recurrence. Methods: In this study, We extracted transcriptome data for 189 pathologically confirmed pT1-2 node-negative TNBC patients at Fudan University Shanghai Cancer Center. Candidate mRNAs were filtered, which was followed by differential expressed mRNAs analysis, survival analysis, and LASSO Cox regression model. All-subsets regression program was used for constructing a multi-mRNA signature in the training set (n=159); the accuracy and prognostic value were then validated using an independent validation set (n=158). Results: Here, we profiled the transcriptome data from 189 early-stage TNBC patients along with 50 paired normal tissues, and developed a prognostic signature based on seven mRNAs (ACAN, KRT5, TMEM101, LCA5, RPP40, LAGE3, CDKL2).In both the training (n=159) and validation cohorts (n=158), the signature could identify patients with relatively high recurrence risks and serve as an independent prognostic factor. Furthermore, the signature had better prognostic value than traditional clinicopathological features in both sets. Among the seven mRNAs, TMEM101 was identified as a prognostic biomarker of early-stage TNBC. Additional cell experiments suggested that TMEM101 could facilitate migration and proliferation of TNBC cells. Conclusions: Our 7-mRNA signature could accurately predict recurrence risks of early-stage TNBCs. Clinical and genomic low risk TNBC patients may safely avoid adjuvant chemotherapy.
Drought Stress Modified Genetic Components and Combining Ability of Cotton Genotypes&nbsp;
Authors: Areej JAVAID , AZHAR Faqir Muhammad , KHAN Iftikhar Ahmad , Amir SHAKEEL , Azhar Muhammad Tehseen 
Published: April 2021

Abstract
Background: Gene action and combining ability of the genotypes are two important components in crop breeding and may be influenced by environmental stresses which sometimes mislead the selection process. Therefore, an experiment was conducted to assess the behavior of genetic parameters of cotton (Gossypium hirsutum) under drought stress during the year 2017-19. Initially, 50 genotypes of cotton were screened for drought stress tolerance potential by subjecting them to 4 week long drought stress (at 20% field capacity). Based on physiological parameters, 5 drought tolerant and 4 drought susceptible genotypes (lines and testers respectively) were selected and hybridized using Line × Tester technique to develop F1 crosses. In the next year, 9 parents and their 20 F1 hybrids were evaluated in field under normal irrigation and drought stress (at 50% field capacity). Results: Stronger specific combining ability effects observed for seed cotton yield, number of bolls, boll weight and lint percentage (%) indicated active role of non-additive genes operating in the direction of lower parents with the exception for number of bolls where dominance worked towards superior parents. Best varietal combinations identified under water stress for number of bolls and boll weight were CIM-496 × NF-801-2-37, DPL-26 × NF-801-2-37, B-557 × NF-801-2-37, B-557 × MNH-129. Variety B-557 was found to be good general combiner followed by BOU-1724 for number of bolls, boll weight and lint % under drought. Conclusion: Combining ability analysis indicated critical role of non-additive component in total variation suggesting weak heritability for all the traits. Direct selection in this material is therefore not advisable. Drought had suppressed variability and restricted the expression of yield characters, however, best identified varietal combinations for seed cotton yield, number of bolls and boll weight may be exploited for the development of hybrid to be grown in drought hit areas of Pakistan.
α-HBDH&nbsp;Is a Probably Higher Sensitive and Specific the Biomarkers of Poor Prognosis in Patients With COVID-19
Authors: hongjun zhang , Xing Gu , Gangqiang Qi , Yanjun Zhao , Xiaoyan Zhang 
Published: April 2021

Abstract
Background: The coronavirus disease 2019 (COVID-19) that is caused by the severe acute respiratory syndrome-coronavirus2 (SARS-CoV2) has spread rapidly worldwide during the past nearly a year. SARS-CoV-2 particles spread through the respiratory mucosa and infect other cells, causing a storm of cytokines in the body1, producing a series of immune responses, and causing multiple organ dysfunction, including the heart. Some patients present with cardiovascular system damage, such as palpitations and shortness of breath as the first or secondary symptoms. Previous studies suggested that LDH, α-HBDH, CK and CK-MB reflect myocardial function.2 Here, we aim to investigate whether these markers can predict poor prognosis of patients with COVID-19. Methods: We collected data from 2338 patients with laboratory-confirmed COVID-19. Patients were then screened, and we focused on 49 moderate cases, 98 severe cases and 53 critical cases (27 recovered cases, 26 deaths). We divided these patients into non-critical group (n = 49) and critical group (n = 151). Then, we also divided the length of hospitalization into five time points, namely admission, 25%, 50%, 75% and discharge or death, according to the principle of interquartile distance. Blood was collected from patients on the above five time points. Patients with five blood tests were 49 moderate cases, 98 severe cases and 53 critical cases (27 recovered cases, 26 deaths). LDH, α-HBDH, CK and CK-MB of each group were collected for analysis.Results: Our research found that α-HBDH and LDH of the critical groups significantly increased, diagnostic efficiency of LDH and α-HBDH have more advantages than that of CK and CK-MB compared with the non-critical group, and patients with α-HBDH greater than 182IU/L and LDH greater than 250IU/L at admission had lower survival rates. Then, CK, LDH, α-HBDH and CK-MB were observed dynamically in the 49 moderate cases, 98 severe cases and 53 critical cases (27 recovered cases, 26 deaths). It turns out that they increased progressively in the dead patients, while they decreased regularly in the severe case and the critical cases(recovered)(P＜0.001).Conclusions: The prognosis of patients with abnormal α-HBDH was poor on admission. α-HBDH is a probably higher sensitive and specific the biomarkers of poor prognosis in patients with COVID-19. This predictor may help clinicians identify patients with a poor prognosis and may be useful for guiding clinical decision-making at an early stage.
Storage of Pineapple Shoot Tips in Liquid Nitrogen for Three Years Does Not Modify Field Performance of Recovered Plants
Authors: Villalobos-Olivera Ariel , Entensa Ysmel , Martínez Julia , Escalante Doris , Quintana Nicolás , Souza Fernanda V. D. , Martínez-Montero Marcos Edel , Hajari Elliosha , Feijoo José Carlos Lorenzo 
Published: April 2021

Abstract
Pineapple is the third most important tropical fruit traded after banana and mango. In recent years, pineapple producers have faced production challenges due to unpredictable weather patterns as a consequence of climate change. In order to develop new genotypes with improved tolerance to these biotic and abiotic stresses, it is necessary to have access to a diverse gene pool, however, the germplasm currently stored in field gene banks are at risk from prevailing environmental conditions. Therefore, there is a need to develop and implement supplementary strategies to conserve plant germplasm under controlled conditions in the laboratory and cryopreservation represents the most viable option for this. To date, methods have been developed for cryopreservation of pineapple shoot tips but there is a lack of information on whether extended periods of storage in liquid nitrogen (LN) has any effect on the regeneration capacity and field performance of cryostored germplasm. Hence, the current study investigated the field performance of pineapple shoot tips after storage for 24 h, one, two and three years. Results were collected following nine months for plant morphological characteristics and 14 months for evaluation of fruit and nutritional characteristics. The results indicated that storage of pineapple shoot tips for up to three years did not have any adverse effects on field performance of plants or on fruit characteristics. This provides evidence that cryopreservation offers a suitable tool for the long term storage of germplasm.
Feasibility, Experiences and Outcomes of using DIALOG+ in Primary Care to Improve Quality of Life and Mental Distress of Patients with Chronic Conditions: An Exploratory Non-Controlled Trial in Bosnia and Herzegovina, Colombia and Uganda
Authors: Loggerenberg Francois van , McGrath Michael , Akena Dickens , Birabwa-Oketcho Harriet , Mendez Camilo Andres Cabarique , Gomez-Restrepo Carlos , Kulenovic Alma Dzubur , Muhic Maja , Sewankambo Nelson K. , Sikira Hana , Priebe Stefan 
Published: April 2021

Abstract
Background: DIALOG+ is a resource-oriented and evidence-based intervention to improve quality of life and reduce mental distress. While it has been extensively studied in mental health care, there is little evidence for how to use it in primary care settings for patients with chronic physical conditions. Considering that DIALOG+ is used in existing routine patient-clinician meetings and is very low cost, it may have the potential to help large numbers of patients with chronic physical conditions, mental distress and poor quality of life who are treated in primary care. This is particularly relevant in low-and-middle-income countries (LMICs) where resources for specialised services for such patients are scarce or non-existent. Methods: An exploratory non-controlled trial will be conducted to assess the acceptability, feasibility and outcomes of delivering DIALOG+ to patients with chronic physical conditions and poor quality of life in primary care settings in Bosnia and Herzegovina, Colombia and Uganda. Thirty patients in each country will receive DIALOG+ up to three times in monthly meetings over a three month period. Feasibility will be assessed by determining the extent to which the intervention is implemented as planned. Experiences will be captured in interviews and focus groups with care providers and participants. As outcomes, quality of life, symptoms of anxiety and depression, objective social situation and health status will be assessed at baseline and again after the three-session intervention. Discussion: This study will inform our understanding of the extent to which DIALOG+ may be used in the routine care of patients with chronic physical conditions in different primary care settings. The findings of this exploratory trial can inform the design of future full randomised controlled trials of DIALOG+ in primary care settings in LMICs. Trial registration: All studies were registered prospectively (on 02/12/2020 for Uganda and Bosnia and Herzegovina, and 01/12/2020 for Colombia) within the ISRCTN Registry. ISRCTN17003451 (Bosnia and Herzegovina), ISRCTN14018729 (Colombia) and ISRCTN50335796 (Uganda). Protocol version and date: v2.0; 28/07/2020 (Bosnia and Herzegovina), v0.3 02/08/2020 (Colombia), and, v1.0, 05/11/2020 (Uganda).
Anaerobic 3-Methylhopanoid Production By An Acidophilic Phototrophic Purple Bacterium
Authors: Mayer Marisa , Parenteau Mary N. , Kempher Megan L. , Madigan Michael T. , Jahnke Linda L. , Welander Paula V. 
Published: April 2021

Abstract
Bacterial lipids are well preserved in ancient rocks and certain ones have been used as indicators of specific bacterial metabolisms or environmental conditions existing at the time of rock deposition. Here we show that an anaerobic bacterium produces 3-methylbacteriohopanepolyols (3-MeBHPs), pentacyclic lipids previously detected only in aerobic bacteria and widely used as biomarkers for methane-oxidizing bacteria. Both Rhodopila globiformis, a phototrophic purple nonsulfur bacterium isolated from an acidic warm spring in Yellowstone, and a newly isolated Rhodopila species from a geochemically similar spring in Lassen Volcanic National Park (USA), synthesized 3-MeBHPs and a suite of related BHPs and contained the genes encoding the necessary biosynthetic enzymes. Our results show that 3-MeBHPs can be produced under anoxic conditions and challenges the use of 3-MeBHPs as biomarkers of oxic conditions in ancient rocks and as prima facie evidence that methanotrophic bacteria were active when the rocks were deposited.
Sleep Disturbances and Their Impact on Cognition in Individuals With Pure Cerebellar Disease
Authors: Sonni Akshata , Schmahmann Jeremy D. , Spencer Rebecca 
Published: April 2021

Abstract
BackgroundIndividuals with cerebellar ataxia commonly report poor sleep. Our objective was to characterize sleep dysfunction in cerebellar ataxia and study its potential impact on non-motor function.MethodsSleep physiology and behavior were measured in 16 individuals with pure cerebellar ataxia and 16 matched controls. Cognitive function was assessed using the Cerebellar Cognitive Affective Syndrome/Schmahmann Scale (CCAS), and a word-pair learning task. We studied performance on the word-pair task before and after a period of overnight sleep to explore whether sleep-dependent memory consolidation is negatively impacted by cerebellar degeneration. ResultsCompared to matched controls, individuals with ataxia experienced greater limb movements during sleep (p=0.048), and increased sleep fragmentation (p=0.009) as measured through polysomnography. Cognitive assessment using the CCAS revealed deficits in executive function in the domains of verbal fluency (p=0.011) and cognitive flexibility (p=0.048). Individuals with ataxia were also impaired in declarative learning, with poor performance on the word-pair association task during Immediate (p=0.021) and Delayed Recall (p=0.011) compared to control participants. Poor sleep impacted cognition: increased sleep fragmentation was correlated with lower scores on the CCAS (p=0.008) and with immediate recall of the word-pair learning task (p=0.001). We found no differences between individuals with ataxia and controls with respect to overnight changes in performance on the word-pair task.ConclusionsSleep integrity is disrupted in individuals with neurodegeneration confined to the cerebellum. Fragmentation of sleep in particular appears to have negative effects on executive function and has practical implications for symptom management.
Impulsive State Estimation for Nonlinear Systems with Redundant Communication Channels and Markov Delay
Authors: Rao Hong-Xia , Guo Yuru , Kuang Ye , Lin Ming , Xu Yong 
Published: April 2021

Abstract
The state estimation issue for the discrete-time nonlinear systems with Markov delay is investigated in this paper, where the redundant communication channel is considered to ensure the reliability of transmission. Because the channel capacity is limited, the packet dropout conditions of the main channel and the redundant channel are described by the Bernoulli stochastic variables. In addition, a mode-dependent estimator is proposed based on the current state and the delayed state, simultaneously. Combining with the impulsive control strategy, the efficiency of estimator is improved. An augmented estimation error system is proposed to deal with the Markov delay in the nonlinear system, subsequently, a sufficient condition that ensures the asymptotic stability of the augmented error system is obtained by a constructed Lyapunov functional candidate and the gains of the impulsive estimator are derived. Finally, a numerical example of moving vehicle is utilized to illustrate the developed results.
Tumor-tumor Cell Fusion Hybrids Obtained by the Modified PHA-DMSO-PEG Method Promote Temozolomide Resistance in Glioma
Authors: Mi Ruifang , Ji Jiayu , Li Mingxin , Zhang Mengmeng , Zhang Junwen , Liu Fusheng 
Published: April 2021

Abstract
Background: Cell fusion and the subsequent aneuploidy are commonly observed in different kinds of tumor. In glioma, cell fusion and the number of the polyploid giant cells were found to be augmented with the tumor grades (WHO Ⅰ-Ⅳ) and closely related to poor prognosis. Phytohemagglutinin (PHA) holds an ability to induce cell-cell membrane contact and accelerates the cell fusion process mediated by the fusogenic agent polyethylene glycol (PEG). Dimethyl sulfoxide (DMSO) is well known as a cryoprotective agent involving in cell cryopreservation. In this study, we aim to obtain the glioma fusion hybrids by the modified fusion method in vitro, and then investigate the pathological consequences and the related molecular mechanism with the cell hybrids.Methods: Glioma cells were labelled by lentiviruses infection. The PEG fusion efficiency was respectively improved by the addition of PHA and DMSO, and quantified by flow cytometry. Then, fusion hybrids were obtained by puromycin screening and fluorescence-activated cell sorting (FACS). Furthermore, DNA content was analyzed through flow cytometry. Cell proliferation rate and cell viability under temozolomide (TMZ) was detected by CCK-8 assay. Lastly, the related gene expression was measured through qRT-PCR and Western blotting. Results: Glioma cell-cell contact was achieved by adding certain concentration of PHA in vitro. Tumor-tumor cell fusion efficiency was improved by PHA and DMSO. Glioma fusion hybrids were successfully obtained after puromycin screening and FACS. Cell size, DNA content and chromosome numbers of the fusion hybrids were almost twice that of the parental glioma cells. Moreover, glioma fusion hybrids showed an enhanced TMZ resistance potential compared to the parental cells, and also the MGMT expression was up-regulated in the hybrids.Conclusions: We successfully obtained the glioma tumor-tumor cell fusion hybrids through the modified PHA-DMSO-PEG fusion method. Cell fusion may contribute to TMZ resistance in glioma, thus inhibition of cell fusion could be a promising orientation to improve TMZ resistance. Moreover, combining TMZ and MGMT inhibitor could be a beneficial approach in patients with glioma polyploid giant cells.
Development of a Novel Human CD147 Transgenic NSG Mouse Model to test SARS-CoV-2 Infection and Immune Responses
Authors: Badeti Saiaditya , Tseng Hsiang-chi , Romanienko Peter , Yehia Ghassan , Liu Dongfang 
Published: April 2021

Abstract
An animal model that can mimic the SARS-CoV-2 infection in humans is critical to understanding the newly emerged, rapidly spreading SARS-CoV-2 and development of therapeutic strategies. Studies show that the spike (S) proteins of SARS-CoV (SARS-CoV-S-1-S) and SARS-CoV-2 (SARS-CoV-2-S) bind to human angiotensin-converting enzyme 2 (hACE2, a well-recognized, functional receptor for SARS-CoV and SARS-CoV-2) to mediate viral entry. Several hACE2 transgenic (hACE2Tg) mouse models are being widely used, which is clearly invaluable. However, the hACE2Tg mouse model cannot fully explain: 1) low expression of ACE2 observed in human lung and heart, but lung or heart failure occurs frequently in severe COVID-19 patients); 2) low expression of ACE2 on immune cells, but lymphocytopenia occurs frequently in COVID-19 patients; and 3) hACE2Tg mice do not develop strong clinical disease following SARS-CoV-2 infection in contrast to SARS-CoV-1. Moreover, one of most outstanding features of coronaviruses is the diversity of receptor usage, which includes the newly proposed human CD147 (hCD147) as a receptor for SARS-CoV-2-S. It is still debatable whether CD147 can serve as a functional receptor for SARS-CoV-2 infection or entry. Here we successfully generated a hCD147Tg mouse model in the NOD-scid IL2Rgammanull (NSG) background. In this hCD147Tg-NSG mouse model, the hCD147 genetic sequence was placed following the endogenous mouse promoter for mouse CD147 (mCD147), which creates an in vivo model that may better recapitulate physiological expression of CD147 proteins at the molecular level compared to the existing and well-studied K18-hACE2-B6 model. In addition, the hCD147Tg-NSG mouse model allows further study of SARS-CoV-2 in the immunodeficiency condition which may assist our understanding of this virus in the context of high-risk populations with immunosuppressed conditions. The hCD147Tg-NSG mouse mode can serve as an additional animal model for interrogate whether CD147 serve as an independent functional receptor or accessory receptor for SARS-CoV-2 entry and immune responses.
Human biting rhythm of Anopheles gambiae s.l. and sleeping behaviour of pregnant women in a lagoon area in Southern Benin
Authors: DJENONTIN Armel , BOURAÏMA Aziz , SOARES Christophe , EGBINOLA Seun , COTTRELL Gilles 
Published: April 2021

Abstract
ObjectiveIn the framework of EVALMOUS study aiming to assess the use and effectiveness of mosquito nets by pregnant women and other members of their household in a lagoon area in southern Benin, the behaviour of pregnant women relative to the time they go to bed using the net were recorded. Malaria vectors biting rhythm, Plasmodium falciparum infection and insecticide resistance genes in malaria vectors were also determined.ResultsOverall, 3848 females of Anopheles gambiae s. l. were collected and 280 pregnant women responded to the survey. Almost all Anopheles gambiae s. l. tested were Anopheles coluzzi with the exception of two, which were Anopheles melas. The CSP index in malaria vector was 1.85% and the allelic frequency of kdr gene was 74.4%. Around 90% of bites and Plasmodium falciparum transmission occurred between 10 p.m. and 6 a.m., which coincides with the period when more than 80% of pregnant women were under bednet. Despite a slight early evening and early morning biting activity of malaria vectors in the study area, the good use of nets might remain a useful protection tool against mosquito biting and malaria transmission.
Even in Simple Economic Systems, Equilibrium Can Be Non-Unique: An Example
Authors: Garcia Nancy Solis , Muniz Jose Guadalupe Flores , Kalashnikov Viacheslav , Kalashnikova Nataliya , Kosheleva Olga 
Published: April 2021

Abstract
According to economics, prices are determined by the relationship between supply and demand: they correspond to the equilibrium in which supply is exactly equal to the demand, i.e., for which the optimal amount that the seller is willing to sell at this price is exactly equal to the optimal amount that the buyer is willing to pay. In many situations, the corresponding prices are indeed uniquely determines by the supply-demand relation. From the purely mathematical viewpoint, there are situations when the equilibrium is not unique, but most economists believe that in practical situations, equilibrium prices are usually uniquely determined. In this paper, we provide a simple but realistic example in which the equilibrium is not unique.
A mobile simulation and ARIMA modeling for prediction of air radiation dose rates
Authors: Salh Hemn , Külahcı Fatih , Aközcan Serpil 
Published: April 2021

Abstract
A spatial simulation method in .mp4 format was proposed to determine Fukushima radioactive fallout transport and the Absorbed Dose Rate, Annual Effective Dose Equivalent, and Excess Lifetime Cancer Risk were determined for 10 months after the accident (March 11 2011). The findings of this study demonstrate that an appropriate ARIMA model can be applied for radiation dose time-series in the case of nuclear reactor accidents like Chernobyl and Fukushima to predict the future air dose rates, which can provide valuable information in determining the evacuation zones, decontamination processes, and radiation protection progresses. The model forecasted results and the actual observation data in the same period shows a gradual decrease in the air dose rates during the prediction period. Moreover, there is a good agreement between them as the prediction and observation scatter plot follows each other with small variations. These results provide important insights into the predictability of ARIMA models; thus, the models were utilized to forecast the air dose rates for the period (January 2020 - October 2020).