bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unaccompanied minors’ experiences of narrative exposure therapy
Authors: Said Glorianne , Alqadri Yaman , King Dorothy 
Published: March 2021

AbstractDespite the understanding that unaccompanied minors’ (UAM) experience high rates of post-traumatic stress, the provision of evidence-based trauma-focused therapies is low for this population. Narrative exposure therapy (NET) is an effective short-term intervention for treating post-traumatic stress disorder (PTSD) after multiple traumatic experiences, such as those experienced by UAM. Within the existing literature, there is a lack of research investigating unaccompanied minors’ experiences of NET or any trauma-focused therapy. Participants were four UAM experiencing PTSD who formed part of a pilot delivery of NET within a dedicated child and adolescent mental health service for refugee children. Semi-structured interviews were conducted and transcripts were analysed using interpretative phenomenological analysis (IPA). This project identified five themes that encapsulated unaccompanied minors’ experiences of receiving NET, including the process of preparing for this therapy, what it was like to receive it, and the differences they identified at the end of treatment. The significance of this taking place within a safe therapeutic relationship was explored within the context of the attachment losses experienced by UAM, and the impact this has on emotion regulation was considered. The potential of a reduction in PTSD symptoms facilitating a positive spiral in adolescence was reflected on within this paper.Key learning aims(1)To understand the experience of unaccompanied asylum-seeking minors (UAM) receiving narrative exposure therapy (NET) for post-traumatic stress disorder.(2)To understand the key concerns and motivators for UAM when considering engaging in NET.(3)To understand how these experiences relate to theoretical frameworks and the existing literature relating to emotional difficulties in adolescence.
The dichotomous and incomplete adaptive immunity in COVID-19 patients with different disease severity
Authors: Gao Leiqiong , Zhou Jing , Yang Sen , Wang Lisha , Chen Xiangyu , Yang Yang , Li Ren , Pan Zhiwei , Zhao Jing , Li Zhirong , Huang Qizhao , Tang Jianfang , Hu Li , Liu Pinghuang , Zhang Guozhong , Chen Yaokai , Ye Lilin 
Published: March 2021

AbstractThe adaptive immunity that protects patients from coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is not well characterized. In particular, the asymptomatic patients have been found to induce weak and transient SARS-CoV-2 antibody responses, but the underlying mechanisms remain unknown; meanwhile, the protective immunity that guide the recovery of these asymptomatic patients is elusive. Here, we characterized SARS-CoV-2-specific B-cell and T-cell responses in 10 asymptomatic patients and 64 patients with other disease severity (mild, n = 10, moderate, n = 32, severe, n = 12) and found that asymptomatic or mild symptomatic patients failed to mount virus-specific germinal center (GC) B cell responses that result in robust and prolonged humoral immunity, assessed by GC response indicators including follicular helper T (TFH) cell and memory B cell responses as well as serum CXCL13 levels. Alternatively, these patients mounted potent virus-specific TH1 and CD8+ T cell responses. In sharp contrast, patients of moderate or severe disease induced vigorous virus-specific GC B cell responses and associated TFH responses; however, the virus-specific TH1 and CD8+ T cells were minimally induced in these patients. These results, therefore, uncovered the protective immunity in asymptomatic patients and also revealed the strikingly dichotomous and incomplete humoral and cellular immune responses in COVID-19 patients with different disease severity, providing important insights into rational design of effective COVID-19 vaccines.
How do established developmental risk-factors for schizophrenia change the way the brain develops?
Authors: Eyles Darryl W. 
Published: March 2021

AbstractThe recognition that schizophrenia is a disorder of neurodevelopment is widely accepted. The original hypothesis was coined more than 30 years ago and the wealth of supportive epidemiologically data continues to grow. A number of proposals have been put forward to suggest how adverse early exposures in utero alter the way the adult brain functions, eventually producing the symptoms of schizophrenia. This of course is extremely difficult to study in developing human brains, so the bulk of what we know comes from animal models of such exposures. In this review, I will summarise the more salient features of how the major epidemiologically validated exposures change the way the brain is formed leading to abnormal function in ways that are informative for schizophrenia symptomology. Surprisingly few studies have examined brain ontogeny from embryo to adult in such models. However, where there is longitudinal data, various convergent mechanisms are beginning to emerge involving stress and immune pathways. There is also a surprisingly consistent alteration in how very early dopamine neurons develop in these models. Understanding how disparate epidemiologically-validated exposures may produce similar developmental brain abnormalities may unlock convergent early disease-related pathways/processes.
Predicting the public health impact of a malaria transmission-blocking vaccine
Authors: Challenger Joseph D. , Olivera Mesa Daniela , Da Dari F. , Yerbanga R. Serge , Lefèvre Thierry , Cohuet Anna , Churcher Thomas S. 
Published: March 2021

AbstractTransmission-blocking vaccines that interrupt malaria transmission from humans to mosquitoes are being tested in early clinical trials. The activity of such a vaccine is commonly evaluated using membrane-feeding assays. Understanding the field efficacy of such a vaccine requires knowledge of how heavily infected wild, naturally blood-fed mosquitoes are, as this indicates how difficult it will be to block transmission. Here we use data on naturally infected mosquitoes collected in Burkina Faso to translate the laboratory-estimated activity into an estimated activity in the field. A transmission dynamics model is then utilised to predict a transmission-blocking vaccine’s public health impact alongside existing interventions. The model suggests that school-aged children are an attractive population to target for vaccination. Benefits of vaccination are distributed across the population, averting the greatest number of cases in younger children. Utilising a transmission-blocking vaccine alongside existing interventions could have a substantial impact against malaria.
Real-time tracking and prediction of COVID-19 infection using digital proxies of population mobility and mixing
Authors: Leung Kathy , Wu Joseph T. , Leung Gabriel M. 
Published: March 2021

AbstractDigital proxies of human mobility and physical mixing have been used to monitor viral transmissibility and effectiveness of social distancing interventions in the ongoing COVID-19 pandemic. We develop a new framework that parameterizes disease transmission models with age-specific digital mobility data. By fitting the model to case data in Hong Kong, we are able to accurately track the local effective reproduction number of COVID-19 in near real time (i.e., no longer constrained by the delay of around 9 days between infection and reporting of cases) which is essential for quick assessment of the effectiveness of interventions on reducing transmissibility. Our findings show that accurate nowcast and forecast of COVID-19 epidemics can be obtained by integrating valid digital proxies of physical mixing into conventional epidemic models.
Extrusion of subducted crust explains the emplacement of far-travelled ophiolites
Authors: Porkoláb Kristóf , Duretz Thibault , Yamato Philippe , Auzemery Antoine , Willingshofer Ernst 
Published: March 2021

AbstractContinental subduction below oceanic plates and associated emplacement of ophiolite sheets remain enigmatic chapters in global plate tectonics. Numerous ophiolite belts on Earth exhibit a far-travelled ophiolite sheet that is separated from its oceanic root by tectonic windows exposing continental crust, which experienced subduction-related high pressure-low temperature metamorphism during obduction. However, the link between continental subduction-exhumation dynamics and far-travelled ophiolite emplacement remains poorly understood. Here we combine data collected from ophiolite belts worldwide with thermo-mechanical simulations of continental subduction dynamics to show the causal link between the extrusion of subducted continental crust and the emplacement of far-travelled ophiolites. Our results reveal that buoyancy-driven extrusion of subducted crust triggers necking and breaking of the overriding oceanic upper plate. The broken-off piece of oceanic lithosphere is then transported on top of the continent along a flat thrust segment and becomes a far-travelled ophiolite sheet separated from its root by the extruded continental crust. Our results indicate that the extrusion of the subducted continental crust and the emplacement of far-travelled ophiolite sheets are inseparable processes.
Modeled microgravity alters lipopolysaccharide and outer membrane vesicle production of the beneficial symbiont Vibrio fischeri
Authors: Vroom Madeline M. , Rodriguez-Ocasio Yaneli , Lynch Jonathan B. , Ruby Edward G. , Foster Jamie S. 
Published: March 2021

AbstractReduced gravity, or microgravity, can have a pronounced impact on the physiology of animals, but the effects on their associated microbiomes are not well understood. Here, the impact of modeled microgravity on the shedding of Gram-negative lipopolysaccharides (LPS) by the symbiotic bacterium Vibrio fischeri was examined using high-aspect ratio vessels. LPS from V. fischeri is known to induce developmental apoptosis within its symbiotic tissues, which is accelerated under modeled microgravity conditions. In this study, we provide evidence that exposure to modeled microgravity increases the amount of LPS released by the bacterial symbiont in vitro. The higher rates of shedding under modeled microgravity conditions are associated with increased production of outer-membrane vesicles (OMV), which has been previously correlated to flagellar motility. Mutants of V. fischeri defective in the production and rotation of their flagella show significant decreases in LPS shedding in all treatments, but levels of LPS are higher under modeled microgravity despite loss of motility. Modeled microgravity also appears to affect the outer-membrane integrity of V. fischeri, as cells incubated under modeled microgravity conditions are more susceptible to cell-membrane-disrupting agents. These results suggest that, like their animal hosts, the physiology of symbiotic microbes can be altered under microgravity-like conditions, which may have important implications for host health during spaceflight.
Dimensional transformation of chemical bonding during crystallization in a layered chalcogenide material
Authors: Saito Yuta , Hatayama Shogo , Shuang Yi , Fons Paul , Kolobov Alexander V. , Sutou Yuji 
Published: March 2021

AbstractTwo-dimensional (2D) van der Waals (vdW) materials possess a crystal structure in which a covalently-bonded few atomic-layer motif forms a single unit with individual motifs being weakly bound to each other by vdW forces. Cr2Ge2Te6 is known as a 2D vdW ferromagnetic insulator as well as a potential phase change material for non-volatile memory applications. Here, we provide evidence for a dimensional transformation in the chemical bonding from a randomly bonded three-dimensional (3D) disordered amorphous phase to a 2D bonded vdW crystalline phase. A counterintuitive metastable “quasi-layered” state during crystallization that exhibits both “long-range order and short-range disorder” with respect to atomic alignment clearly distinguishes the system from conventional materials. This unusual behavior is thought to originate from the 2D nature of the crystalline phase. These observations provide insight into the crystallization mechanism of layered materials in general, and consequently, will be useful for the realization of 2D vdW material-based functional nanoelectronic device applications.
Serotonin deficiency induced after brain maturation rescues consequences of early life adversity
Authors: Aboagye B. , Weber T. , Merdian H. L. , Bartsch D. , Lesch K. P. , Waider J. 
Published: March 2021

AbstractBrain serotonin (5-HT) system dysfunction is implicated in depressive disorders and acute depletion of 5-HT precursor tryptophan has frequently been used to model the influence of 5-HT deficiency on emotion regulation. Tamoxifen (TAM)-induced Cre/loxP-mediated inactivation of the tryptophan hydroxylase-2 gene (Tph2) was used to investigate the effects of provoked 5-HT deficiency in adult mice (Tph2 icKO) previously subjected to maternal separation (MS). The efficiency of Tph2 inactivation was validated by immunohistochemistry and HPLC. The impact of Tph2 icKO in interaction with MS stress (Tph2 icKO × MS) on physiological parameters, emotional behavior and expression of 5-HT system-related marker genes were assessed. Tph2 icKO mice displayed a significant reduction in 5-HT immunoreactive cells and 5-HT concentrations in the rostral raphe region within four weeks following TAM treatment. Tph2 icKO and MS differentially affected food and water intake, locomotor activity as well as panic-like escape behavior. Tph2 icKO prevented the adverse effects of MS stress and altered the expression of the genes previously linked to stress and emotionality. In conclusion, an experimental model was established to study the behavioral and neurobiological consequences of 5-HT deficiency in adulthood in interaction with early-life adversity potentially affecting brain development and the pathogenesis of depressive disorders.
A first-passage approach to diffusion-influenced reversible binding and its insights into nanoscale signaling at the presynapse
Authors: Reva Maria , DiGregorio David A. , Grebenkov Denis S. 
Published: March 2021

AbstractSynaptic transmission between neurons is governed by a cascade of stochastic calcium ion reaction–diffusion events within nerve terminals leading to vesicular release of neurotransmitter. Since experimental measurements of such systems are challenging due to their nanometer and sub-millisecond scale, numerical simulations remain the principal tool for studying calcium-dependent neurotransmitter release driven by electrical impulses, despite the limitations of time-consuming calculations. In this paper, we develop an analytical solution to rapidly explore dynamical stochastic reaction–diffusion problems based on first-passage times. This is the first analytical model that accounts simultaneously for relevant statistical features of calcium ion diffusion, buffering, and its binding/unbinding reaction with a calcium sensor for synaptic vesicle fusion. In particular, unbinding kinetics are shown to have a major impact on submillisecond sensor occupancy probability and therefore cannot be neglected. Using Monte Carlo simulations we validated our analytical solution for instantaneous calcium influx and that through voltage-gated calcium channels. We present a fast and rigorous analytical tool that permits a systematic exploration of the influence of various biophysical parameters on molecular interactions within cells, and which can serve as a building block for more general cell signaling simulators.
Homological scaffold via minimal homology bases
Authors: Guerra Marco , De Gregorio Alessandro , Fugacci Ulderico , Petri Giovanni , Vaccarino Francesco 
Published: March 2021

AbstractThe homological scaffold leverages persistent homology to construct a topologically sound summary of a weighted network. However, its crucial dependency on the choice of representative cycles hinders the ability to trace back global features onto individual network components, unless one provides a principled way to make such a choice. In this paper, we apply recent advances in the computation of minimal homology bases to introduce a quasi-canonical version of the scaffold, called minimal, and employ it to analyze data both real and in silico. At the same time, we verify that, statistically, the standard scaffold is a good proxy of the minimal one for sufficiently complex networks.
Direct evidence of microstructure dependence of magnetic flux trapping in niobium
Authors: Balachandran Shreyas , Polyanskii Anatolii , Chetri Santosh , Dhakal Pashupati , Su Yi-Feng , Sung Zu-Hawn , Lee Peter J. 
Published: March 2021

AbstractElemental type-II superconducting niobium is the material of choice for superconducting radiofrequency cavities used in modern particle accelerators, light sources, detectors, sensors, and quantum computing architecture. An essential challenge to increasing energy efficiency in rf applications is the power dissipation due to residual magnetic field that is trapped during the cool down process due to incomplete magnetic field expulsion. New SRF cavity processing recipes that use surface doping techniques have significantly increased their cryogenic efficiency. However, the performance of SRF Nb accelerators still shows vulnerability to a trapped magnetic field. In this manuscript, we report the observation of a direct link between flux trapping and incomplete flux expulsion with spatial variations in microstructure within the niobium. Fine-grain recrystallized microstructure with an average grain size of 10–50 µm leads to flux trapping even with a lack of dislocation structures in grain interiors. Larger grain sizes beyond 100–400 µm do not lead to preferential flux trapping, as observed directly by magneto-optical imaging. While local magnetic flux variations imaged by magneto-optics provide clarity on a microstructure level, bulk variations are also indicated by variations in pinning force curves with sequential heat treatment studies. The key results indicate that complete control of the niobium microstructure will help produce higher performance superconducting resonators with reduced rf losses1 related to the magnetic flux trapping.
The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
Authors: Hieda Miki , Matsumoto Taizo , Isobe Mari , Kurono Sadamu , Yuka Kaneko , Kametaka Satoshi , Wang Jing-Ya , Chi Ya-Hui , Kameda Kenji , Kimura Hiroshi , Matsuura Nariaki , Matsuura Shuji 
Published: March 2021

AbstractThe morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the outer nuclear membrane nesprin proteins, which comprise the LINC complex. We found that depletion of SUN1 leads to Golgi complex dispersion with maintenance of ministacks and retained function for vesicle transport through the Golgi complex. In addition, SUN2 associates with microtubule plus-end-directed motor KIF20A, possibly via nesprin-2. KIF20A plays a role in the Golgi dispersion in conjunction with the SUN2-nesprin-2 LINC complex in SUN1-depleted cells, suggesting that SUN1 suppresses the function of the SUN2-nesprin-2 LINC complex under a steady-state condition. Further, SUN1-knockout mice, which show impaired cerebellar development and cerebellar ataxia, presented altered Golgi morphology in Purkinje cells. These findings revealed a regulation of the Golgi organization by the LINC complex.
Thermodynamic mechanism evaluate the feasibility of oil shale pyrolysis by topochemical heat
Authors: Zhao Shuai , Lü Xiaoshu , Sun Youhong , Huang Jiandong 
Published: March 2021

AbstractTopochemical heat in-situ pyrolysis of oil shale is achieved by injecting high temperature nitrogen to promote oil shale pyrolysis and release heat, and then injecting air to trigger oil shale combustion in the early stage of oil shale pyrolysis, and then by injecting normal temperature air continuously to promote local oxidation of oil shale in the later stage. In order to verify the oil and gas recovery by topochemical heat method, Jilin University has chosen Fuyu City, Jilin Province, to carry out pilot project of oil shale in-situ pyrolysis by topochemical heat method. Besides, in order to infer the spontaneity, feasibility and difficulty of continuous pyrolysis of oil shale based on topochemical heat, this paper, the mechanism of solid-state pyrolysis and the thermodynamic analysis of transition state of oil shale in Fuyu area are discussed. Because the second stage of oil shale pyrolysis is the main stage of oil production. Therefore, the characteristics of Gibbs free energy, free enthalpy and free entropy of transition state in the main oil production stage of oil shale pyrolysis are obtained by calculation. The results show that in situ pyrolysis of oil shale topochemical heat can be carried out spontaneously and continuously, and the release characteristics of volatiles during pyrolysis of oil shale are described.
The in-vitro effect of famotidine on SARS-CoV-2 proteases and virus replication
Authors: Loffredo Madeline , Lucero Hector , Chen Da-Yuan , O’Connell Aoife , Bergqvist Simon , Munawar Ahmad , Bandara Asanga , De Graef Steff , Weeks Stephen D. , Douam Florian , Saeed Mohsan , Munawar Ali H. 
Published: March 2021

AbstractThe lack of coronavirus-specific antiviral drugs has instigated multiple drug repurposing studies to redirect previously approved medicines for the treatment of SARS-CoV-2, the coronavirus behind the ongoing COVID-19 pandemic. A recent, large-scale, retrospective clinical study showed that famotidine, when administered at a high dose to hospitalized COVID-19 patients, reduced the rates of intubation and mortality. A separate, patient-reported study associated famotidine use with improvements in mild to moderate symptoms such as cough and shortness of breath. While a prospective, multi-center clinical study is ongoing, two parallel in silico studies have proposed one of the two SARS-CoV-2 proteases, 3CLpro or PLpro, as potential molecular targets of famotidine activity; however, this remains to be experimentally validated. In this report, we systematically analyzed the effect of famotidine on viral proteases and virus replication. Leveraging a series of biophysical and enzymatic assays, we show that famotidine neither binds with nor inhibits the functions of 3CLpro and PLpro. Similarly, no direct antiviral activity of famotidine was observed at concentrations of up to 200 µM, when tested against SARS-CoV-2 in two different cell lines, including a human cell line originating from lungs, a primary target of COVID-19. These results rule out famotidine as a direct-acting inhibitor of SARS-CoV-2 replication and warrant further investigation of its molecular mechanism of action in the context of COVID-19.