bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of the allergenic characteristics and serodiagnostic potential of recombinant chitinase-like protein-5 and -12 from Sarcoptes scabiei
Authors: Shen Nengxing , Chen Yuhang , Wei Wenrui , Xiong Lang , Tao Yuanyuan , Xiao Jie , Liu Song , He Xue , Du Xiaodi , Gu Xiaobin , Xie Yue , Xu Jing , Peng Xuerong , Yang Guangyou 
Published: March 2021

Abstract
Background
Scabies is caused by burrowing of the mite Sarcoptes scabiei into the stratum corneum. Currently, diagnosis via routine skin scraping is very difficult, and information on the allergenic identification of S. scabiei remains limited.

Methods
We performed comparative analysis of the serological diagnostic potential of recombinant S. scabiei chitinase-like protein-5 (rSsCLP5) and recombinant S. scabiei chitinase-like protein-12 (rSsCLP12) by measuring the levels of serum-specific IgG and IgE antibodies (Abs) as diagnostic markers. In addition, the allergenic characteristics of rSsCLP5 and rSsCLP12 were evaluated using IgE-binding experiments and skin tests.

Results
The IgE Abs-based indirect enzyme-linked immunosorbent assay (ELISA) methods showed high sensitivity and specificity: the rSsCLP5-based assay had 93.5% sensitivity and 94.4% specificity; the rSsCLP12-based assay had 100% sensitivity and 98.1% specificity. The specific IgE Abs in infested mouse sera could bind rSsCLP5 and rSsCLP12. In skin tests, rabbits in the rSsCLP5 and rSsCLP12 groups and positive control (histamine) groups exhibited allergic reactions. Most test sites in the rSsCLP12 group had edema, bleeding spots, and even ulcers or scabs, but such allergy symptoms were rare in the rSsCLP5 group. Moreover, the allergic history rabbit group had more severe allergic reactions and lower levels of IgE Abs compared to the healthy rabbit group in the same protein group.

Conclusions
These findings validate the use of IgE Abs to rSsCLP5 and rSsCLP12 as potentially useful markers for diagnosing scabies. Moreover, both rSsCLP5 and rSsCLP12 have allergenic properties, and the potential allergen rSsCLP12 is a stronger allergen than rSsCLP5.

Graphical Abstract
HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis
Authors: Wang Zheng , Zhou Nan , Wang Wengang , Yu Yangke , Xia Lei , Li Ning 
Published: March 2021

Abstract
Background
Osteoarthritis (OA) is a disabling joint disease that causes articular cartilage degeneration. It has been implicated that altered expression of histone deacetylase 2 (HDAC2) is found in patients with OA. However, the specific role of HDAC2 in the development of OA still remains enigmatic. Hence, we sought to characterize the functional relevance of HDAC2 in the development of OA.

Methods
Anterior cruciate ligament surgery was performed to generate the rat model of OA. Luciferase assay was performed to evaluate the relationship between microRNA-503-5p (miR-503-5p) and serum- and glucocorticoid-inducible kinase-1 (SGK1). Functional experiments were conducted to examine the functional significance of miR-503-5p, histone deacetylase 2 (HDAC2), and SGK1 on the progression of OA by determining proliferation, apoptosis, and expression of apoptosis-associated proteins and inflammatory cytokines.

Results
HDAC2 could inhibit miR-503-5p expression. SGK1 was the target gene of miR-503-5p. Upregulation of miR-503-5p or silencing of HDAC2 contributed to enhanced proliferation, suppressed apoptosis (reduced expression of Caspase-3 and Bax but elevated expression of Bcl2), and promoted inflammation in chondrocytes of OA rats.

Conclusion
In conclusion, our study demonstrated that HDAC2 could promote OA through miR-503-5p/SGK1 axis, which might function as a therapeutic target for OA treatment.
Developed channel propagation models and path loss measurements for wireless communication systems using regression analysis techniques
Authors: Zakaria Yahia A. , Hamad Ehab K. I. , Elhamid A. S. Abd , El-Khatib K. M. 
Published: March 2021

Abstract
Background
The development of powerful and flexible management addresses is due to the improvement of deeply reliable gadgets and the advancement of the concept of cellular. The cellular principle was a major way of solving the wavelength crowding problem and the user capacity. It offered high capacity without major technological changes with limited allocation in spectrum. Wireless communication is an innovation in media transmission that allows remote transmission in all types of terrain between convenient gadgets. For estimating a transmitter 's radio coverage area, propagation models that anticipate the mean signal strength for an arbitrary transmitter–receiver separation distance are crucial as they are called large-scale propagation models so even though they define the average signal strength over long periods of time and large distances from the transmitter.

Results
Developed propagation models are presented according to the measured path loss values in exemplary urban and suburban areas at the operating frequency of 3.5 ghz by using the regression analysis. The measurements are implemented by using a spectrum analyzer FSH6 to get the channel response as shown in the stated tables and graphs. Based on the obtained results, it was observed that the path loss could be calculated as a function of distance and during the practical measurements 32 m was precise as a presumption for the break point distance. The values of path loss exponent (n) are defined and calculated for both of urban and suburban regions. Measurements results are analyzed and compared in order to study their influence for every specific environment. It was noticed that any radio signal will suffer attenuation when it travels from the transmitter to the receiver as a variety of various phenomena give rise to this radio path loss.

Conclusions
The interaction between both the electromagnetic radiation and the environment tends to decrease the quality of the signal being sent from the transmitter to the destination which causes the loss of the path. Propagation models are the basis for channel estimation, as they attempt to identify how a radio transmission changes from the transmitter to the receiver throughout its path. The gained results from this research will be supportive for the arrangement of network planners and researchers as proof and directory materials before future location establishment.
Discovery of Bis-sydnone styryl ketone as a selective cyclooxygenase-2 inhibitor
Authors: Abdualkader Abdualrahman Mohammed , Taher Muhammad , Nik Yusoff Nik Idris , Alaama Mohamed 
Published: March 2021

AbstractBackgroundVarious literature sources have documented a wide spectrum of therapeutic properties of sydnones including anti-inflammatory, anticancer, antimicrobial activities. Phenyl styryl ketones and their derivatives as members of the chalcone family have also been reported as significant bioactive molecules. The current study was initiated to evaluate the anti-inflammatory activity of sydnone-based compounds including some novelbis-sydnone styryl ketone hybrids.ResultsTwenty-five sydnone-containing compounds were successfully synthesized. Compounds46-48and56-58were reported as new sydnone derivatives. Whereas, compounds61-63were synthesized as novel molecules containing two sydnone rings linked via α,β-unsaturated ketone. The structures of the synthesized compounds were confirmed by FTIR,1H NMR,13C NMR and ToF-MS analyses. The in vitro COX inhibition assay showed varied activity. Compounds47,51,58and63showed the most potent COX inhibitory effects at a concentration of 200 μM. The selectivity index revealed that63was the best selective COX-2 inhibitor. Acetylation of the sydnone ring at C-4 was fruitful for the COX inhibitory effects. Docking analysis showed that COX-2 selectivity was due to a favourable positive charged interaction occurring between the sydnone ring of63and Arg513 of COX-2. Compound51was hydrogen bonded to Arg513. On the other hand, the low inhibitory effect of63against COX-1 was due to an unfavourable polar interaction with His513 in the binding pocket of COX-1.ConclusionsThe compounds were successfully synthesized and characterized. Compound63had a common architecture and pharmacophoric features with known selective COX-2 inhibitors (the coxib family) which make it a suitable candidate for the designing of selective and safe NSAID.
Improving Care for Patients With Stage III or IV NSCLC: Learnings for Multidisciplinary Teams From the ACCC National Quality Survey
Authors: Salgia Ravi , Boehmer Leigh M. , Celestin Catherine , Yu Hong , Spigel David R. 
Published: March 2021

PURPOSE: Insufficient characterization of the optimal multidisciplinary team and lack of understanding of barriers to quality care are unmet needs in the management of stage III or IV non–small-cell lung cancer (NSCLC). A national survey was conducted to inform the design and execution of process improvement plans and address identified barriers. METHODS: A steering committee of multidisciplinary specialists and representation from patient advocacy collaborated for a comprehensive, double-blind, web-based survey (January-April 2019) to obtain insights on care delivery for patients with advanced NSCLC in a diverse set of US community cancer programs. RESULTS: Overall, 639 responses (160 unique cancer programs across 44 US states) were included; 41% (n = 261) of respondents indicated an absence of a thoracic multidisciplinary clinic in their cancer program. Engagement in shared decision making was significantly associated with the presence of navigation and radiation oncology disciplines ( P ≤ .04); 19.2% and 33.3% of respondents belonged to cancer programs with no lung cancer screening and no protocol for biomarker testing, respectively. The frequency of tumor board meetings negatively correlated with time to complete disease staging ( P = .03); the average time to first therapeutic intervention in newly diagnosed patients was 4 weeks. The most challenging barriers to quality care included insufficient quantity of biopsy material for biomarker testing, lack of primary care provider referrals, and diagnostic costs. CONCLUSION: Improving the quality of advanced NSCLC care, including optimization of a multidisciplinary team framework, may surmount barriers to care coordination, diagnosis and staging, and treatment planning, consequently improving adherence to evolving standards of care.
Primary Ovarian Insufficiency in Women With Addison’s Disease
Authors: Vogt Elinor C , Breivik Lars , Røyrvik Ellen C , Grytaas Marianne , Husebye Eystein S , Øksnes Marianne 
Published: March 2021

Abstract

Context
Primary ovarian insufficiency (POI) is defined by menopause before 40 years of age. POI prevalence is higher among women with autoimmune Addison’s disease (AAD) than in the general population, but their clinical characteristics are insufficiently studied.

Objective
To assess the prevalence of POI in a large cohort of women with AAD and describe clinical, immunological, and genetic characteristics.

Methods
An observational population-based cohort study of the Norwegian National Addison Registry. The Norwegian Prescription Database was used to assess prescription of menopausal hormone replacement therapy (HRT). A total of 461 women with AAD were studied. The primary outcome measure was prevalence of POI. Secondary outcomes were clinical characteristics, autoantibodies, and genome-wide single nucleotide polymorphism variation.

Results
The prevalence of POI was 10.2% (47/461) and one-third developed POI before 30 years of age. POI preceded or coincided with AAD diagnosis in more than half of the women. The prevalence of concomitant autoimmune diseases was 72%, and AAD women with POI had more autoantibodies than AAD women without (≥2 autoantibodies in 78% vs 25%). Autoantibodies against side-chain cleavage enzyme (SCC) had the highest accuracy with a negative predictive value for POI of 96%. HRT use was high compared to the age adjusted normal population (11.3 % vs 0.7%).

Conclusion
One in 10 women with AAD have POI. Autoantibodies against SCC are the most specific marker for autoimmune POI. We recommend testing women with AAD &lt;40 years with menstrual disturbances or fertility concerns for autoantibodies against SCC.
A dupla excepcionalidade superdotação e TDA/H: uma revisão da literatura
Authors: Marques Ogeda Clarissa Maria , Pedro Ketilin Mayra , Martins Bárbara Amaral 
Published: March 2021

Estudantes com comportamento superdotado podem apresentar traços semelhantes aos característicos do Transtorno do Déficit de Atenção/Hiperatividade (TDAH), ocasionando diagnósticos equivocados. No entanto, a coexistência desses fenômenos também é possível. Objetivou-se identificar e caracterizar as produções científicas que abordam a associação entre superdotação e TDAH. Para isso, foram consultadas cinco bases de dados: Biblioteca Digital Brasileira de Teses e Dissertações, Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Portal de Periódicos do Scientific Eletronic Library Online, Literatura Latino-Americana e do Caribe em Ciências da Saúde e na base de dados Education Resources Information Center. Foram usados os descritores gifted e adhd, hyperactivity e attention deficit hyperactivity disorder em inglês, e também foram adotados “altas habilidades”, “superdotação”, “talento”, “dotação” e “precocidade”, juntamente com o descritor “TDAH”, “transtorno de déficit de atenção/hiperatividade” e “hiperatividade”. Assim, foram localizadas 33 produções. A categorização e análise dos trabalhos revelou a existência de diferenças no funcionamento de estudantes com comportamento superdotado quando em associação ao TDAH, as quais afetam aspectos educacionais, socioemocionais e requerem intervenção específica. Maiores investigações acerca dessa temática são necessárias para evitar erros diagnósticos..
O acervo da Teias em foco: as perspectivas e contribuições da revista através de meta-análises
Authors: Semaan Gustavo Silva , Silva Edelberto Franco , Brito José André De Moura 
Published: March 2021

Com o objetivo de conhecer novas perspectivas em relação ao acervo da Revista TEIAS, em destaque pela alta qualidade de seus trabalhos e de excelência confirmada pelo sistema de avaliação Qualis da CAPES, o trabalho analisa sua comunidade por meio das redes de colaborações e contribuições. Criada no ano 2000 com foco na área de conhecimento Educação, em seu 21º ano de atuação superou a marca de 1.000 artigos publicados por mais de 1.300 autores. Para a realização das meta-análises foi considerado o processo de Descoberta de Conhecimento em Bases de Dados e conceitos de Teoria dos Grafos. Os resultados obtidos possuem estatísticas gerais, análises das redes de colaboração, termos em destaque ao passar dos anos e autores considerados influentes com base em sua frequência de publicação na revista, pela quantidade de autores com quem colaboram bem como por meio do uso da medida de centralidade por intermediação. Assim, o presente trabalho fornece informações e análises importantes, que podem ser consideradas em outras pesquisas e apoiam interpretações, perspectivas e contribuições deste importante acervo.
Managing BVDV at the herd level part 2
Authors: Button Ellie 
Published: March 2021

Bovine viral diarrhoea virus (BVDV) is a costly disease and its eradication is the focus of the English voluntary scheme, BVDFree. The process of identifying a farm as negative or not negative is broken down into four stages using ADAM: assess disease risk; define herd status; action plan for BVD control; monitor progress. This process is based on the long-established protocols defined by Cattle Health Certification Standards. The latter two stages are discussed in this article with the first two discussed in part 1 of this series. Initial testing will identify the farm as negative or not negative for BVDV. Herds that are not negative will require further testing, which will often take the form of screening for a persistently infected (PI) animal, or ‘PI hunt’, by testing every animal in the herd either directly or indirectly for the presence of virus. Maintaining and monitoring BVDV status will require ongoing testing, tag and test, or check test at the farmer's choice, and continued attention to biosecurity, especially in negative herds that are not vaccinated.
Models for charging in practice: how should farm veterinary surgeons earn their living?
Authors: Atkinson Owen 
Published: March 2021

There are many different ways that practices use for charging farm clients for their professional services. Ideally, the model is equitable for veterinary surgeon, client and animal. Different structures can influence how a practice's services are used and which services are successful. This article, which is an opinion piece, reviews common fee charging structures in UK farm practice. An argument is presented that if practices progressed towards a subscription-style charging model, at least for advisory work, and possibly for out-of-hours emergency work, this could be beneficial to farm businesses, veterinary businesses, farm veterinary surgeons, and the animals under their care.
Multi-plane confocal microscopy with multiplexed volume holographic gratings [Invited]
Authors: Chia Chou-Min , Vyas Sunil , Wu Tso-Hua , Yeh J. Andrew , Luo Yuan 
Published: March 2021

A volume holographic (VHG) grating-based multi-plane differential confocal microscopy (DCM) is proposed for axial scan-free imaging. Also, we briefly reviewed our previous works on volume holographic-based confocal imaging. We show that without degrading imaging performance, it is possible to simultaneously obtain two depth-resolved optically sectioned images with improved axial resolution using multi-plane DCM. The performance of our multi-plane DCM was evaluated by measuring the surface profile of a silicon micro-hole array with depths separation around 10 µm. The axial sensitivity of the system is around 25 nm. Our system has the advantages of multi-plane imaging with high axial sensitivity and high optical sectioning ability. Our method can be used for reflective surface profiling and multi-plane fluorescence imaging. The present methods may find important applications in surface metrology for label-free biological samples, as well as industrial applications.
Ultrafast soliton mode-locked fiber laser at 1560  nm based on
Znq2 as a saturable absorber
Authors: Salam Sameer , Nizamani Bilal , Azooz Salam M. , Fizza G. , Yasin M. , Harun S. W. 
Published: March 2021

A mode-locked fiber laser was developed by using bis(8-hydroxyquinoline) zinc (

Z
n

q

2

) as a saturable absorber (SA).

Z
n

q

2

is an organic metal chelate with several advantages such as good saturable absorption, relatively high damage threshold, and an easy, low-cost, and simple fabrication process. The developed SA based on

Z
n

q

2

has a linear absorption (at 1560 nm), modulation depth, and saturation intensity of 1.2 dB, 16.6 %, and

95

M
W

/

c
m

2

, respectively. The laser cavity produced a very stable soliton mode-locking operation at 1560.6 nm with pulse duration, repetition rate, and SNR of 1.46 ps, 3.5 MHz, and 60.6 dB, respectively. This work is the first, to the best of our knowledge, to report

Z
n

q

2

as a mode locker.
Mid-infrared electro-optic modulator based on a graphene-embedded plasmonic rib waveguide with ultrahigh electro-optic wavelength tuning
Authors: Hou Chenyang , Ma Tao , Gao Jinhui , Liu Heng , Wang Fang 
Published: March 2021

A graphene-embedded plasmonic rib waveguide (GEPRW) is designed for the mid-infrared
electro-optic modulator. The mode characteristics and electro-optic
(EO) modulation performances are simulated and optimized by using the
finite element method. The results show that propagation length of

10

3

m
m

and figure of merit of

10

6

are obtained by adjusting the bias
voltage applied to the GEPRW. The EO wavelength tunings are

−

66.69

and

−

78.87

n
m

/

V

for peak

L

and peak

R

in the loss spectra when

w
=

3

µ

m

and

h
1

=

2

µ

m

. For a 100 µm long GEPRW, the
modulation depths of

∼

96.4

,

∼

97.1

,

∼

93.7

, and

∼

94.9

%

, and FWHMs of

∼

30

,

∼

74

,

∼

34

, and

∼

59

n
m

can be achieved when

λ
=

1.55

, 1.87, 1.89, and 2.23 µm. The EO
modulator based on the GEPRW has a wide wavelength tuning range from
1.05 to 2.23 µm. It has high modulation depth, low insertion loss, and
broad bandwidth, which can be used as EO tunable devices such as
optical interconnects and optical switches.
Raman spectrometer for high precision temperature sensing of
atmospheric gases
Authors: Mashburn Carter , Borysow Jacek 
Published: March 2021

We present a highly accurate Raman spectrometer capable of measuring
changes in atmospheric temperature as small as 0.75 K with high
spatial and temporal resolution. The spectrometer is based on a laser
diode tuned to the resonant absorption line of the

85

R
b

isotope near 780.0 nm. A heated glass
cell containing Rb atoms was used as an ultranarrowband atomic
absorption notch filter with

0.3

c

m

−
1

bandwidth and optical density as high
as four. This filter was placed in front of the spectrograph and
blocked up to 99.99% of the elastically scattered laser light, which
made it possible to resolve the pure-rotational Raman spectra of
molecular atmospheric gases. The relative intensities of
pure-rotational Raman transitions were then used to infer atmospheric
temperature changes.
Self-stabilized soliton generation in a microresonator through mode-pulled Brillouin lasing
Authors: Do In Hwan , Kim Dohyeong , Jeong Dongin , Suk Daewon , Kwon Dohyeon , Kim Jungwon , Lee Jae Hoon , Lee Hansuek 
Published: March 2021

Reducing the complexity required for starting and maintaining a soliton state has been a major task to fully miniaturize soliton microcombs including the accompanying external operating systems. Here we experimentally examine the generative process of a self-stabilized soliton in which a continuous-wave pump detuned on the thermally stable blue side of a resonance generates a Brillouin lasing signal that relays the pump power to the soliton pulses via intracavity mode-coupling without breaking thermal self-stability. Based on a simple setup consisting of a free-running laser and a microcavity without any external feedback systems by virtue of internal thermal locking, single-soliton pulses of 11 GHz repetition rate were deterministically generated. We demonstrate that the single-soliton pulses can be passively maintained over several days in a laboratory environment with a phase noise performance of

−

137

d
B
c

/

H
z

at 100 kHz.
Nonlinear refractive index of solids in mid-infrared
Authors: Patwardhan Gauri N. , Ginsberg Jared S. , Chen Cecilia Y. , Jadidi M. Mehdi , Gaeta Alexander L. 
Published: March 2021

We measure the third-order nonlinear optical response of various dielectrics and semiconductors using the spectrally resolved two-beam coupling method at 2.3 µm, 3.5 µm, 4.5 µm, and 8.3 µm. These materials include fused silica, sapphire, calcium fluoride, magnesium fluoride, zinc sulphide, and zinc selenide. We compare our results with previous literature results and theoretically expected values using two-band model theory. The dispersion of the nonlinear refractive index

n
2

over this wavelength range is found to be negligible.