bluuebunny/crossref_metadata_2025_split huggingface dataset:
An aberrant venous channel mimicking the perilabyrinthine cells in the petrous bone of a patient with vestibular schwannoma: illustrative case
Authors: Ito Masato , Higuchi Yoshinori , Horiguchi Kentaro , Nakano Shigeki , Origuchi Shinichi , Aoyagi Kyoko , Serizawa Toru , Yamakami Iwao , Iwadate Yasuo 
Published: November 2021

BACKGROUND
Anatomical variations, such as high jugular bulbs and air cell development in the petrosal bone, should be evaluated before surgery. Most bone defects in the internal auditory canal (IAC) posterior wall are observed in the perilabyrinthine cells. An aberrant vascular structure passing through the petrous bone is rare.

OBSERVATIONS
A 48-year-old man presented with a right ear hearing disturbance. Magnetic resonance imaging revealed a 23-mm contrast-enhancing mass in the right cerebellopontine angle extending into the IAC, consistent with a right vestibular schwannoma. Preoperative bone window computed tomographic scans showed bone defects in the IAC posterior wall, which ran farther posteroinferiorly in the petrous bone, reaching the medial part of the jugular bulb. The tumor was accessed via a lateral suboccipital approach. There was no other major vein in the cerebellomedullary cistern, except for the vein running from the brain stem to the IAC posterior wall. To avoid complications due to venous congestion, the authors did not drill out the IAC posterior wall or remove the tumor in the IAC.

LESSONS
Several aberrant veins in the petrous bone are primitive head sinus remnants. Although rare, their surgical implication is critical in patients with vestibular schwannomas.
Experimental study on co-culture of DiI labeled rat bone marrow mesenchymal stem cells and polycaprolactone film in vitro to make a cell patch
Authors: Zichang Zhang , Fan Zhou , Jianwei Zheng , Junsheng Mu , Ping Bo , Bin You 
Published: October 2021

BACKGROUND: In stem cell therapy, due to the lack of an effective carrier, a large number of transplanted stem cells are lost and die. Therefore, finding a suitable carrier has become a further direction of stem cell therapy. OBJECTIVE: In research on the co-culture of polycaprolactone (PCL) with 1,1′-Dioctadecyl-3,3,3′,3′- tetramethylindocarbocyanine perchlorate (DiI) labeled bone marrow mesenchymal stem cells (BMSCs), we observe the effect of materials on the growth and proliferation of DiI labeled stem cells, and the effect of DiI labeling on patch preparation, so as to find a kind of biomaterial suitable for the growth and proliferation of BMSCs, and find a suitable cell carrier for stem cell therapy of myocardial infarction and in vivo tracing. METHODS: Clean grade Sprague Dawley rats were selected as experimental objects, BMSCs were isolated and cultured, and the surface markers were identified by flow cytometry. After the BMSCs were cultured for 3 passages, the BMSCs were stained with DiI dye, and the BMSCs DiI and PCL biomaterial film were co-cultured. After 24 hours, the cell growth was observed under fluorescence microscope, and fixed for scanning under electron microscope. The cell proliferation was detected by CCK-8 at 1, 4, 7, 10 days of culture. The measurement data conforming to normal distribution are expressed in the form of mean ± standard deviation (X¯± s). One way ANOVA was used for comparison among groups, LSD analysis was used for pairwise comparison. The difference was statistically significant (P < 0.05). RESULTS: BMSCs were strongly positive for CD90, CD44H, but negative for CD11b/c, CD45. Under fluorescence microscope, BMSCs DiI showed red light, fusiform or polygonal. Under the scanning electron microscope, the cell patch formed by co-culture of PCL film and DiI-BMSCs had a large number of cells on the surface and normal cell state. CCK-8 assay showed that the OD value on the first day was 0.330 ± 0.025; The OD value was 0.620 ± 0.012 on the 4th day, 1.033 ± 0.144 on the 7th day and 1.223 ± 0.133 on the 10th day. There was significant difference among the time points (P < 0.05). CONCLUSIONS: The cell patch made of PCL film and DiI labeled BMSCs can survive and proliferate on the surface, so it can be used as a scaffold material for stem cell therapy in vivo.
Feasibility and efficiency of contrast enhanced ultrasound real time guided fine needle aspiration for sentinel lymph node of breast cancer
Authors: Shi Xian-Quan , Zhang Huiming , Liu Xi , Dong Yunyun , Yang Peipei , Qian Linxue 
Published: October 2021

AIM: To assess the feasibility and efficiency of contrast-enhanced ultrasound (CEUS) real-time guided fine needle aspiration (FNA) for sentinel lymph node (SLN) of breast cancer. MATERIALS AND METHODS: This retrospective study reviewed 21 breast cancer patients who scheduled for surgical resection performed CEUS real-time guided SLN-FNA and intraoperative SLN biopsy (SLNB). The success rate of CEUS real-time guided SLN-FNA was analyzed. The FNA diagnostic efficiency of SLN metastasis was analyzed compared to SLNB. RESULTS: Twenty-six SLNs were detected by intradermal CEUS whereas 130 SLNs were detected by SLNB. The median SLNs detected by intradermal CEUS ( n = 1) and by SLNB ( n = 5) was significantly difference ( p < 0.001). All 26 CE-SLNs of 21 patients were successfully performed intradermal CEUS dual image real-time guided SLN-FNA including 5 SLNs of 4 patients which were difficult to distinguish in conventional ultrasound. Compared to SLNB, FNA found 2 of 5 cases of SLN metastasis, the diagnosis sensitivity, specificity, positive predictive value, negative predictive value, false negative rate, false positive rate and Yoden index were 40%, 100%, 100%, 84.2%, 60%, 0%and 40%, respectively. CONCLUSION: SLN-FNA real-time guided by dual CEUS image mode was technically feasible. Patients with a positive SLN-FNA should be advised to ALND without intraoperative SLNB according to Chinese surgeon and patients’ conservatism attitude. But a negative SLN-FNA did not obviate the need of conventional SLNB because of the high false negative rate.
Management of Early Breast Cancer at an Australian Cancer Centre During the Early Phase of COVID-19 Pandemic
Authors: J Zhang Elissa , Stuart Kirsty , Hui Rina , Mellor Rhiannon , Wang Wei , Ahern Verity , Meybodi Farid , French James , Elder Elisabeth , Brennan Meagan 
Published: November 2021

Background: This study aimed to prospectively record changes to treatment for early breast cancer patients during the first wave of the COVID-19 pandemic in Australia. The purpose was to assess the impact on breast cancer outcomes and to determine the need for any mitigative actions.
Methods: The study was conducted in the breast cancer unit of a tertiary referral hospital. Patients with early (non-metastatic) breast malignancy discussed in multidisciplinary team meetings between March and June 2020 were included. Patients were newly diagnosed, post-operative or post-neoadjuvant chemotherapy. Standard treatment was defined by Westmead Breast Cancer Institute protocols and any variations related to the pandemic were recorded.
Results: In the study, 145 patients were included (median age 59 years). Pandemic-related changes to management were noted in 13 of 145 (9.0%) patients. Four patients experienced a delay to cancer treatments, four were not offered reconstructive/ symmetrisation surgical procedures, three had altered radiotherapy protocols and two patients were not offered enrolment to a clinical trial. These impacts affected the groups presenting with new cancers (n=7/86, 8.1%), post-operative cases (n=4/25, 16.0%) and post-neoadjuvant chemotherapy cases presenting for surgical planning (n=2/34, 5.9%).
Conclusion: Most patients (91.0%) received standard treatment during the first wave of the pandemic. The minor variations from institutional protocols observed in this study are unlikely to affect local control or survival in this patient cohort, but close follow-up is required. Quality of life may have been affected for four patients who had downgraded or delayed reconstructive procedures.
Analisa Kelayakan Bisnis Tas Tenun Heritage di Bogor
Authors: Sonny Sonny , Prabawa Muhamad Surya 
Published: November 2021

<p align="center"><strong>ABSTRAK</strong><strong> </strong></p><p>Penelitian ini merupakan analisis kelayakan bisnis tas tenun ikat merek Heritage di Bogor. Analisa kelayakan terdiri dari dua analisa, yaitu analisis industri dan perencanaan keuangan. Industri akan dianalisa menggunakan <em>SWOT analysis</em>. Dari sisi keuangan, akan dianalisa menggunakan <em>Return on Asset (ROA</em>) dan <em>Payback Period</em> (PP). Perhitungan ROA didapatkan hasil 33%, menghasilkan laba bersih positif sebesar Rp. 96,232,321 ; PP dalam jangka waktu 3,1 tahun. Kesimpulan penelitian ini dari aspek industri dan finansial, bisnis Heritage ini layak untuk dijalankan.</p><p> </p><p align="center"><strong><em>ABSTRACT</em></strong><em></em></p><p><em>This study is an business feasibility of Heritage’s brand woven bag business in Bogor. The feasibility consists of two analysis, namely industrial analysis and financial planning. The industry will analyze using SWOT analysis. From the financial side, it will be analyzed using Return on Assets (ROA) and Payback Period (PP). ROA calculation results obtained 33%, resulting in a positive net profit of Rp. 96,232,321; and PP for a period of 3.1 years (less than 5 years). The conclusion of this research from the industrial and financial aspects, the Heritage woven bag business is feasible to run.</em><em></em></p><p> </p>
Analisa kekuatan pada poros roda depan motor honda blade 110R tahun 2010 dengan material St90 JIS SCM 447 – AISI 4340
Authors: Aji Alharisy Aji 
Published: November 2021

<p><em>Saat ini di indonesia sepeda motor merupakan alat tranportasi yang sudah di produksi massal. penggunaan sparepart sepeda motor antara yang merek satu dengan merek yang lainnya sudah umum dilakukan dikarenakan komponen tersebut terkadang sulit didapatkan di pasaran. Entah karena sparepart sepeda motor tersebut tidak lagi diproduksi oleh pabrikan atau memang ada faktor-faktor lain yang mempengaruhinya. Poros adalah salah satu komponen sepeda motor , dimana poros merupakan salah satu bagian yang terpenting dari setiap mesin. Peranan utama sebuah poros adalah untuk mentransmisikan daya dari satu elemen mesin ke bagian elemen mesin lainnya. Daya tersebut dihasilkan oleh gaya tangensial dan momen torsi yang hasil akhirnya adalah daya tersebut akan ditransmisikan kepada elemen lain yang terhubung dengan poros tersebut. Poros roda merupakan salah satu komponen yang saangat penting dari sebuah sepeda motor karena poros berfungsi untuk menopang body, beban kendaraan itu sendiri maupun beban luar pada kendaraan dalam hal ini manusia atau barang muatan pada sepeda motor. Sehingga di perlukan poros yang baik untuk mencapai fungsi dari poros tersebut. Dipenelitian ini akan membahas tentang Poros dengan beban puntir dan lentur yang bertujuan untuk memberikan pengetahuan tentang poros depan, Lebih menambah wawasan pengetahuan dalam bidang otomotif dan Mampu merencanakan secara sistematis elemen-elemen mesin yang ada pada system kerja poros. Perhitungan Beban Luar, Perhitungan Diameter Poros, Jadi hasil dari perhitungan poros bahwa diameter 9.9 mm aman digunakan sesuai dengan standard, dengan diameter poros actual dilapangan yaitu 10 mm</em></p>
Lesões por pressão provocadas por dispositivos médicos em Unidades de Terapia Intensiva
Authors: Lopes Vanessa Verçosa , Moura Jaciara Pereira de , Silva Juan Victor da , Silva Államy Danilo Moura e 
Published: November 2021

Objetivo: buscar evidências científicas relacionadas aos agravantes das lesões por pressões causada por dispositivos médicos; analisar por meio da literatura a relação dos dispositivos médicos para o surgimento de lesões por pressão e identificar condutas da assistência de enfermagem que contribuam para a prevenção de lesões por pressão. Surgiu a necessidade de aprofundar-se na análise dessa área da enfermagem, que possui grande relevância, buscando maneiras de melhorar a assistência de enfermagem no cuidado com pacientes críticos. Métodos: Revisão integrativa da literatura realizada nas bases de dados Medical Literature Analysis and Retrieval System Online (MEDLINE/PUBMED), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) e Base de Dados de Enfermagem (BDENF), utilizando a Biblioteca Virtual em Saúde (BVS). Resultados: Os 12 estudos que compuseram essa revisão foram realizados de 2015 a 2020, sendo 1 (8,3%) em 2016, 2 (16,7%) em 2017, 1 (8,3%) em 2018, 5 (41,7%) em 2019 e 3 (25,0%) em 2020. Em relação a abordagem, 6 (50,0%) foram estudos descritivos, 4 (33,3%) descritivos exploratórios, 1 (8,3%) exploratório e 1 (8,3%) quase experimental. Conclusão: com o estudo que as lesões por pressão relacionadas a dispositivos médicos é um evento com incidência elevada em unidades de terapia intensiva visto que, em pacientes gravemente enfermos, a prevenção de úlceras por pressão é um desafio devido ao grande risco de múltiplas comorbidades, o maior uso de dispositivos médicos dentre outros fatores relacionados.
A influência do dentifrício na abrasividade da estrutura dentinária: uma revisão narrativa
Authors: Queiroz Alessandra Soares de , Santos Isabela Rodrigues dos , Martins Victor da Mota , Andrade Claudia Maria de Oliveira , Dietrich Lia , Nascimento Fernando , Reis Tais Alves dos 
Published: November 2021

A escovação dos dentes é considerada o método mais comum para manter uma boa higiene oral. No entanto, os dentifrícios demonstraram uma manifestação de desgaste dentário erosivo. Mesmo que um dentifrício não leve a nenhuma perda de superfície significativa, a estrutura do tecido duro pode tornar vulnerável à abrasão. O objetivo deste estudo foi analisar como os componentes das pastas dentais agem para o desgaste das estruturas dentárias. As bases de dados utilizadas foram PubMed e Scielo com as palavras chave: “dental abrasion”, “toothpastes”, “erosion”. A busca se deu por artigos publicados de 2004 a 2021. A literatura revisada demonstrou que os dentifrícios com finalidade clareadora causam desgaste significativo da estrutura dental, devido aos altos níveis de abrasivos em sua composição, porém os dentifrícios convencionais também mostraram desgaste dentário em menor proporção. er Além disso, a hipersensibilidade dentinária tem sido considerada um problema decorrente da perda de estrutura dentária. Por isso, foi apresentado dentifrícios com efeito anti-erosivo com finalidade de prevenir a sensibilidade dentária. Cada dentifrício existente no mercado tem uma composição diferente dependendo do seu objetivo, e um nível abrasivo específico. Cabe ao Cirurgião-Dentista saber prescrever de forma correta o uso desses dentifrícios de acordo com a necessidade de cada paciente promovendo uma saúde bucal adequada.
Miíase em região submandibular direita: relato de caso
Authors: Santos Beatriz Nogueira dos , Costa Lucas Leverson Lisboa da , Lemos Alice Christinne de Alencar , Costa Amanda Marinho Chaves , Soares Analícia Costa , Silva Letícia Sandes de Albuquerque , Albuquerque Maria Júlia Ventura de , Nogueira Rudson da Silva , Souza Darllon Manuel Borges de , Moreira Lucya Giselle Costa , Macedo Lucas Fortes Cavalcanti de 
Published: November 2021

Introdução: A miíase é uma condição patológica caracterizada pela infestação de larvas dípteras em tecidos vivos ou cavidades necróticas, essas larvas se desenvolvem como parasitas sendo alimentadas pelos tecidos do hospedeiro. Tal afecção pode ser classificada em primária, que consiste no desenvolvimento das larvas a partir do tecido vivo (biófago), ou secundária, que é caracterizada por seu desenvolvimento a partir do tecido morto (necrófagos). Objetivo: relatar um caso de miíase submandibular em região direita, tratada com ivermectina posteriormente à terapia cirúrgica. Relato de caso: Paciente do gênero masculino, 46 anos, compareceu ao Hospital Geral do Estado com queixa de lesão em região submandibular. Ao exame clínico e de imagem foi possível confirmar o diagnóstico de miíase. Em seguidao o paciente foi conduzido ao centro cirúrgico, onde foi realizado a remoção mecânica das larvas, seguida de desinfecção da lesão e prescrição medicamentosa. A lesão respondeu bem ao tratamento com ivermectina 150 μg/kg, administrada por via oral, em dose única. Conclusão: Com isso, foi observado que a remoção de todas as larvas associada à terapia antibiótica, consiste em um bom tratamento para paciente com miíase em região oral e maxilofacial.
PENGARUH HOLDING TIMEDAN MEDIA PENDINGIN PADA PROSES HARDENING TERHADAP KEKERASAN DAN LAJU KOROSI BAJA AISI 410
Authors: Budi Muhammad Ichlasul Rafif Setia , Subagyo  
Published: November 2021

Baja AISI 410 merupakan baja tahan karat martensitik yang sangat sering dijumpai baik di bidang industri ataupun kesehatan salah satunya dapa dijumpai pada komponen blade system turbin uap. Tujuan dari peneltian ini adalah untuk mengetahui pengaruh holding time dan konsentrasi garam media pendingin terhadap kekerasan dan laju korosi baja AISI 410 hasil hardening. Dengan penelitian eksperimental dimana baja AISI 410 di-hardening dengan cara dipanaskan hingga suhu 950oC, di tahan atau holding dengan variasi 10, 20, 30 menit, lanjut dengan quenching menggunakan air garam dengan variasi konsentrasi 0, 10, 20, 30%, dan selanjutnya diuji kekerasan dengan metode rockwell c dan uji laju korosi dengan metode kehilangan berat . Hasilnya menunjukkan bahwa baja AISI 410 hasil hardening dengan variasi holding time menghasilkan nilai kekerasan tertinggi dan laju korosi terbaik pada holding time 30 menit dengan nilai kekerasan 46,5 HRC dan nilai laju korosi 0,8968 MPY, sedangkan untuk variasi konsentrasi kadar garam media pendingin menghasilkan nilai kekerasan tertinggi dan laju korosi terbaik pada konsentrasi 30% dengan nilai kekerasan 45,9 HRC dan nilai laju korosi 1,1036 MPY
PENGARUH VARIASI WAKTU PENAHANAN DAN TEBAL BENDA KERJA HASIL QUENCHING TERHADAP SIFAT MEKANIK BAJA STAINLESS STEEL MARTENSIT
Authors: Saragih Dian Juanda Philip , Subagyo  
Published: November 2021

Baja tahan karat martensitik merupakan salah satu baja paduan yang paling sering dijumpai dalam menjalani kehidupan sehari-hari, seperti turbin blade, instrumen bedah dan lain sebagainya. Baja tahan karat martensitik, terlihat jelas yang berarti memiliki sifat tahan karat atau korosi menjadikan baja ini dapat dijadikan struktur martensit yang keras sehingga dapat digunakan sebagai bahan utama untuk pembuatan alat-alat keras yang tahan korosi dan aus. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh waktu penahanan dan ketebalan benda kerja terhadap sifat mekanik pada baja tahan karat martensitik pada proses hardening. Untuk metode penelitian ini menggunakan metode experimental untuk menguji pengaruh waktu penahanan dan ketebalan benda kerja terhadap sifat mekanik baja tahan karat matensitik. Hasil pada penelitian ini menunjukkan bahwa baja AISI 410 hasil hardening dengan variasi holding time menghasilkan nilai kekerasan tertinggi dan nilai impact yang paling baik didapatkan pada holding time 30 menit dengan nilai kekerasan 448 HV dan nilai impact 0,146 J/mm2, sedangkan untuk variasi ketebalan benda menghasilkan nilai kekerasan tertinggi dan nilai impact yang paling didapatkan pada ketebalan benda 2,5 mm dengan nilai kekerasan 449 HV dan nilai impact 0,17 J/mm2.
Kata kunci : Quenching, sifat mekanik, stainless steel martensit, ketebalan benda, waktu penahanan
Catechol 1,2-Dioxygenase From Paracoccus sp. MKU1—A Greener and Cleaner Bio-Machinery for cis, cis-Muconic Acid Production by Recombinant E. coli
Authors: Aravind Manikka Kubendran , Varalakshmi Perumal , John Swamidoss Abraham , Ashokkumar Balasubramaniem 
Published: November 2021

Cis, cis-muconic acid (ccMA) is known for its industrial importance as a precursor for the synthesis of several biopolymers. Catechol 1,2-dioxygenase (C12O) is involved in aromatic compounds catabolism and ccMA synthesis in a greener and cleaner way. This is the first study on C12O gene from a metabolically versatile Paracoccus sp. MKU1, which was cloned and expressed in E. coli to produce ccMA from catechol. From the E. coli transformant, recombinant C12O enzyme was purified and found to be a homotrimer with a subunit size of 38.6 kDa. The apparent Km and Vmax for C12O was 12.89 µM and 310.1 U.mg−1, respectively, evidencing high affinity to catechol than previously reported C12Os. The predicted 3D-structure of C12O from MKU1 consisted of five α-helices in N-terminus, one α-helix in C-terminus, and nine β-sheets in C-terminus. Moreover, a unique α-helix signature ‘EESIHAN’ was identified in C-terminus between 271 and 277 amino acids, however the molecular insight of conservative α-helix remains obscure. Further, fed-batch culture was employed using recombinant E. coli expressing C12O gene from Paracoccus sp. MKU1 to produce ccMA by whole-cells catalyzed bioconversion of catechol. With the successive supply of 120 mM catechol, the transformant produced 91.4 mM (12.99 g/L) of ccMA in 6 h with the purity of 95.7%. This single step conversion of catechol to ccMA using whole-cells reactions of recombinants did not generate any by-products in the reaction mixtures. Thus, the recombinant E. coli expressing high activity C12O from Paracoccus sp. MKU1 holds promise as a potential candidate for yielding high concentrations of ccMA at faster rates in low cost settings.
miR-6807-5p Inhibited the Odontogenic Differentiation of Human Dental Pulp Stem Cells Through Directly Targeting METTL7A
Authors: Wang Ning , Han Xiao , Yang Haoqing , Xia Dengsheng , Fan Zhipeng 
Published: November 2021

Background: Tooth tissue regeneration mediated by mesenchymal stem cells (MSCs) has become the most ideal treatment. Although the known regulatory mechanism and some achievements have been discovered, directional differentiation cannot effectively induce regeneration of tooth tissue. In this study, we intended to explore the function and mechanism of miR-6807-5p and its target gene METTL7A in odontogenic differentiation.Methods: In this study, human dental pulp stem cells (DPSCs) were used. Alkaline phosphatase (ALP), Alizarin red staining (ARS), and calcium ion quantification were used to detect the odontogenic differentiation of miR-6807-5p and METTL7A. Real-time RT-PCR, western blot, dual-luciferase reporter assay, and pull-down assay with biotinylated miRNA were used to confirm that METTL7A was the downstream gene of miR-6807-5p. Protein mass spectrometry and co-immunoprecipitation (Co-IP) were used to detect that SNRNP200 was the co-binding protein of METTL7A.Results: After mineralized induction, the odontogenic differentiation was enhanced in the miR-6807-5p-knockdown group and weakened in the miR-6807-5p-overexpressed group compared with the control group. METTL7A was the downstream target of miR-6807-5p. After mineralized induction, the odontogenic differentiation was weakened in the METTL7A-knockdown group and enhanced in the METTL7A-overexpressed group compared with the control group. SNRNP200 was the co-binding protein of METTL7A. The knockdown of SNRNP200 inhibited the odontogenic differentiation of DPSCs.Conclusion: This study verified that miR-6807-5p inhibited the odontogenic differentiation of DPSCs. The binding site of miR-6807-5p was the 3′UTR region of METTL7A, which was silenced by miR-6807-5p. METTL7A promoted the odontogenic differentiation of DPSCs. SNRNP200, a co-binding protein of METTL7A, promoted the odontogenic differentiation of DPSCs.