bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell minor-axis length is a critical feature for breast cancer cell migration on straight, wavy, loop and grid microfibre patterns
Authors: Zhang Duo , Sheng Yaqi , Piano Nicholas , Jakuszeit Theresa , Cozens Edward , Dong Lingqing , Lei Iek Man , Wang Wenyu , Terentjev Eugene , Huang Yan Yan Shery 
Published: July 2021

AbstractCell migration plays an important role in physiological and pathological processes where the fibrillar morphology of extracellular matrice (ECM) could regulate the migration dynamics. To mimic the morphological characteristics of fibrillar matrix structures, low-voltage continuous electrospinning was adapted to construct straight, wavy, looped and gridded fibre patterns made of polystyrene (of fibre diameter ca. 3 μm). With microfibres deposited onto non-passivated surfaces, cells were permitted to explore their different shapes in response to the directly-adhered fibre, as well as to the neighbouring patterns. For all the patterns studied, analysing cellular migration dynamics of MDA-MB-231 (a highly migratory breast cancer cell line) demonstrated a switch in behaviour when the pattern features approach the upper limit of the cell minor axis. Our findings suggest that, although cells dynamically adjust their shapes in response to different fibrillar environments during migration, their ability to divert from an existing fibre track is limited by the size along the cell minor axis. We therefore conclude that the upper limit of cell minor axis might act as a guide for the design of microfibre patterns for different purposes of cell migration.
Hyperspectral characterisation of natural illumination in woodland and forest environments
Authors: Shiwen Li , Steel Laura , Dahlsjö Cecilia A. L. , Peirson Stuart N. , Shenkin Alexander , Morimoto Takuma , Smithson Hannah E. , Spitschan Manuel 
Published: July 2021

ABSTRACTLight in nature is complex and dynamic, and varies along spectrum, space, direction, and time. While both spectrally resolved measurements and spatially resolved measurements are widely available, spectrally and spatially resolved measurements are technologically more challenging. Here, we present a portable imaging system using off-the-shelf components to capture the full spherical light environment in a spectrally and spatially resolved fashion. The method relies on imaging the 4π-steradian light field reflected from a mirrored chrome sphere using a commercial hyperspectral camera (400-1000 nm) from multiple directions and an image-processing pipeline for extraction of the mirror sphere, removal of saturated pixels, correction of specular reflectance of the sphere, promotion to a high dynamic range, correction of misalignment of images, correction of intensity compression, erasure of the imaging system, unwrapping of the spherical images, filling-in blank regions, and stitching images collected from different angles. We applied our method to Wytham Woods, an ancient semi-natural woodland near Oxford, UK. We acquired a total of 168 images in two sites with low and high abundance of ash, leading to differences in canopy, leading to a total 14 hyperspectral light probes. Our image-processing pipeline corrected small (<3 °) field-based misalignment adequately. Our novel hyperspectral imaging method is adapted for field conditions and opens up novel opportunities for capturing the complex and dynamic nature of the light environment.
Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa
Authors: Bartholomai Bradley M. , Gladfelter Amy S. , Loros Jennifer J. , Dunlap Jay C. 
Published: July 2021

AbstractSingle molecule RNA-FISH (smFISH) is a valuable tool for analysis of mRNA spatial patterning in fixed cells that is underutilized in filamentous fungi. A primary complication for fixed-cell imaging in filamentous fungi is the need for enzymatic cell wall permeabilization, which is compounded by considerable variability in cell wall composition between species. smFISH adds another layer of complexity due to a requirement for RNase free conditions. Here, we describe the cloning, expression, and purification of a chitinase suitable for supplementation of a commercially available RNase-free enzyme preparation for efficient permeabilization of the Neurospora cell wall. We further provide a method for smFISH in Neurospora which includes a tool for generating numerical data from images that can be used in downstream customized analysis protocols.
Spectral estimation of in-vivo wheat chlorophyll a/b ratio under contrasting water availabilities
Authors: Mulero Gabriel , Bacher Harel , Kleiner Uri , Peleg Zvi , Herrmann Ittai 
Published: July 2021

AbstractTo meet the ever-growing global population necessities, it is needed to identify climate change-relevant plant traits to integrate into breeding programs. Developing new tools for fast and accurate estimation of chlorophyll parameters, chlorophyll a (Chl-a), chlorophyll b (Chl-b) content, and their ratio (Chl-a/b), can promote breeding programs of wheat with enhanced climate adaptively. Spectral reflectance of leaves is affected by changes in pigments concentration and can be used to estimate chlorophyll parameters. The current study identified and validated the top spectral indices known and developed new vegetation indices (VIs) for Chl-a and Chl-b content estimation and used them to non-destructively estimate Chl-a/b values and compare them to hyperspectral estimations. Three wild emmer introgression lines, with contrasting drought stress responsiveness dynamics, were selected. Well and limited irrigation irrigation regimes were applied. The wheat leaves were spectrally measured with a handheld spectrometer to acquire their reflectance at the 330 to 790 nm range. Regression models based on calculated VIs as well as all hyperspectral curves were calibrated and validated against chlorophyll extracted values. The developed VIs resulted in high accuracy of Chl-a and Chl-b estimation allowing indirect non-destructive estimation of Chl-a/b with root mean square error (RMSE) values that could fit 6 to 10 times in the range of the measured values. They also performed similarly to the hyperspectral models. Altogether, we present here a new tool for a non-destructive estimation of Chl-a/b which can serve as a basis for future breeding efforts of climate-resilience wheat as well as other crops.
Mcc1229, an Stx2a-amplifying microcin, is produced in vivo and requires CirA for activity
Authors: Nawrocki Erin M. , Hutchins Laura E. , Eaton Kathryn A. , Dudley Edward G. 
Published: July 2021

AbstractEnterohemorrhagic E. coli (EHEC) strains, including the foodborne pathogen E. coli O157:H7, are responsible for thousands of hospitalizations each year. Various environmental triggers can modulate pathogenicity in EHEC by inducing expression of Shiga toxin (Stx), which is encoded on a lambdoid prophage and transcribed together with phage late genes. Cell-free supernatants of the sequence type (ST) 73 E. coli strain 0.1229 are potent inducers of Stx2a production in EHEC, suggesting that 0.1229 secretes a factor that activates the SOS response and leads to phage lysis. We previously demonstrated that this factor, designated microcin (Mcc) 1229, was proteinaceous and plasmid-encoded. To further characterize Mcc1229 and support its classification as a microcin, we investigated its regulation, determined its receptor, and identified loci providing immunity. Production of Mcc1229 was increased upon iron limitation, as determined by ELISA, lacZ fusions, and qRT-PCR. Spontaneous Mcc1229-resistant mutants and targeted gene deletion revealed that CirA was the Mcc1229 receptor. TonB, which interacts with CirA in the periplasm, was also essential for Mcc1229 import. Subcloning of the Mcc1229 plasmid indicated that Mcc activity was neutralized by two ORFs, each predicted to encode a domain of unknown function (DUF)-containing protein. In a germfree mouse model of infection, colonization with 0.1229 suppressed subsequent colonization of EHEC. Although Mcc1229 was produced in vivo, it was dispensable for colonization suppression. The regulation, import, and immunity determinants identified here are consistent with features of other Mccs, suggesting that Mcc1229 be included in this class of small molecules.
Hydroxyl radical mediated damage of proteins in low oxygen solution investigated using X-ray footprinting mass spectrometry
Authors: Kristensen Line G. , Holton James M. , Rad Behzad , Chen Yan , Petzold Christopher J. , Gupta Sayan , Ralston Corie Y. 
Published: July 2021

In the method of X-ray footprinting mass spectrometry (XFMS), proteins at micromolar concentration in solution are irradiated with a broadband X-ray source, and the resulting hydroxyl radical modifications are characterized using liquid chromatography mass spectrometry to determine sites of solvent accessibility. These data are used to infer structural changes in proteins upon interaction with other proteins, folding, or ligand binding. XFMS is typically performed under aerobic conditions; dissolved molecular oxygen in solution is necessary in many, if not all, the hydroxyl radical modifications that are generally reported. In this study we investigated the result of X-ray induced modifications to three different proteins under aerobic versus low oxygen conditions, and correlated the extent of damage with dose calculations. We observed a concentration-dependent protecting effect at higher protein concentration for a given X-ray dose. For the typical doses used in XFMS experiments there was minimal X-ray induced aggregation and fragmentation, but for higher doses we observed formation of covalent higher molecular weight oligomers, as well as fragmentation, which was affected by the amount of dissolved oxygen in solution. The higher molecular weight products in the form of dimers, trimers, and tetramers were present in all sample preparations, and, upon X-ray irradiation, these oligomers became non-reducible as seen in SDS-PAGE. The results provide an important contribution to the large body of X-ray radiation damage literature in structural biology research, and will specifically help inform the future planning of XFMS, and well as X-ray crystallography and small-angle X-ray scattering experiments.
Have Text, Will Travel: Can Airbnb Use Review Text Data to Optimize Profits?
Authors: Gibbs Shea , Venkatesan Rajkumar 
Published: July 2021

Hundreds of thousands of would-be hoteliers have been popping up all around the world, hoping to rent their own homes and apartments to complete strangers through a service called Airbnb. The goal of Airbnb’s aspiring hosts was to use the company’s website to attract guests who were willing to pay the highest rates to stay in their homes for a short time. For Airbnb, the goal was to improve customer review performance so it could, in turn, increase profits. How could the company achieve its goal? Enter text mining, a technique that allowed businesses to scour Internet pages, decipher the meaning of groups of words, and assign the words a sentiment proxy through the use of a software package.In order for text mining to be useful for Airbnb, its marketing professionals first had to gain access to customer review data on the company’s own website. The team then had to analyze the data to find ways to improve property performance. Was the team going to be able to leverage this large amount of data to determine a strategy going forward?
Linking paternalistic leadership to work engagement among Chinese expatriates: a job demand-resource perspective
Authors: He Guohua , Wang Yanfei , Zheng Xinnian , Guo Zisheng , Zhu Yu 
Published: July 2021

PurposeThis study explores how paternalistic leadership (PL) influences Chinese expatriates' work engagement in a cross-cultural context, and examines how expatriates' cross-cultural adaptability sets a boundary condition for this relationship.Design/methodology/approachData were collected from two-wave surveys of 82 supervisors and 318 Chinese expatriate teachers from 57 Confucius Institutes in 18 countries. Structural equation modeling was used to test the hypothesized relationships.FindingsBenevolent and moral leadership as job resources are negatively related to work–family conflict (WFC), whereas the job demand of authoritarian leadership positively relates to WFC. Further, WFC mediates the effect of PL styles on Chinese expatriates' work engagement. Cross-cultural adaptability moderates the negative relationship between WFC and work engagement, and the indirect effect of PL styles on work engagement through WFC.Practical implicationsOrganizations should consider WFC an important intervening mechanism linking PL and Chinese expatriates' work engagement. Cross-cultural organizations can mitigate the negative impact of WFC on work engagement by enhancing expatriates' cross-cultural adaptability.Originality/valueThis study is the first to examine the PL–work engagement relationship via a work–family interface, which contributes to integrating leadership and work–family outcomes. It enriches research on the JD-R model by showing that job resources and job demands affect employee outcomes through the mediation of stressors. Furthermore, this study identifies a new personal resource by examining cross-cultural adaptability's moderating role.
Short of drugs? Call upon operations and supply chain management
Authors: de Vries Harwin , Jahre Marianne , Selviaridis Kostas , van Oorschot Kim E. , Van Wassenhove Luk N. 
Published: July 2021

PurposeThis “impact pathways” paper argues that operations and supply chain management (OSCM) could help address the worsening drug shortage problem in high-income countries. This significant societal problem poses difficult challenges to stakeholders given the complex and dynamic nature of drug supply chains. OSCM scholars are well positioned to provide answers, introducing new research directions for OSCM in the process.Design/methodology/approachTo substantiate this, the authors carried out a review of stakeholder reports from six European countries and the academic literature.FindingsThere is little academic research and no fundamental agreement among stakeholders about causes of shortages. Stakeholders have suggested many government measures, but little evidence exists on their comparative cost-effectiveness.Originality/valueThe authors discuss three pathways of impactful research on drug shortages to which OSCM could contribute: (1) Developing an evidence-based system view of drug shortages; (2) Studying the comparative cost-effectiveness of key government interventions; (3) Bringing supply chain risk management into the government and economics perspectives and vice versa. Our study provides a baseline for future COVID-19-related research on this topic.
Exploring the application of blockchain to humanitarian supply chains: insights from Humanitarian Supply Blockchain pilot project
Authors: Baharmand Hossein , Maghsoudi Amin , Coppi Giulio 
Published: July 2021

PurposeSome studies and reports have recently suggested using blockchain technology to improve transparency and trust in humanitarian supply chains (HSCs). However, evidence-based studies to display the utility and applicability of blockchains in HSCs are missing in the literature. This paper aims to investigate the key drivers and barriers of blockchain application to HSCs and explore whether evidence could support that the application of blockchain improves transparency and trust in HSCs.Design/methodology/approachThis paper puts forward a two-stage approach to explore the blockchain application in HSCs: an initial exploration of humanitarian practitioners and academicians interested in blockchain through focus group discussions; semi-structured interviews with practitioners involved at the UK Department for International Development's Humanitarian Supply Blockchain pilot project.FindingsFirst, we found that main drivers include accountability, visibility, traceability, trust, collaboration, time efficiency, reducing administrative work and cross-sector partnership. Main barriers, however, are composed of engagement issues, lack of technical skills and training, lack of resources, privacy concerns, regulatory problems, pilot scalability issues and governance challenges. Second, evidence from our case study revealed the blockchain application could have added value to improve visibility and traceability, thus contributing to improve transparency. Concerning trust, evidence supports that blockchain could enhance both commitment and swift trust in the pilot study.Practical implicationsOur study contributes to a more understanding of added values and challenges of blockchain application to HSCs and creates a perspective for humanitarian decision-makers.Originality/valueThis study provides the first evidence from the actual application of blockchain technology in HSCs. The study discovered that it is still less evident in many humanitarian organizations, including medium- and small-sized nongovernmental organizations, that they engage in a direct deployment of in-house or customized blockchain-based HSC. Instead, these actors are more likely to indirectly use blockchain in HSCs through a private commercial partner.
Luxury supply chain management: a framework proposal based on a systematic literature review
Authors: Brandao Maicom Sergio , Godinho Filho Moacir , da Silva Andrea Lago 
Published: July 2021

PurposeThis study aims to identify the main elements that describe the luxury supply chain. It discusses the relationship between them in a framework that organises and summarises the literature.Design/methodology/approachA systematic literature review was conducted that returned 288 papers, which were selected based on specific quality and theme criteria. Content analysis was used to investigate the alignment of critical success factors with the performance goals and configuration elements of luxury supply chains in the final sample of 66 papers.FindingsThe results provide a framework that clarifies the relationship between the configuration elements and supply chain performance goals and the critical success factors for three different levels of the luxury market. Depending on the level of luxury, performance goals and configuration elements assume a different importance and different characteristics. An understanding of these differences is relevant for defining strategies and managing luxury supply chains properly. The three different configurations also reveal new research avenues to be further investigated.Research limitations/implicationsThe study is limited in terms of its data source as the papers reviewed were collected from only three academic databases.Practical implicationsThe findings of this work help incorporate knowledge about luxury supply chain management into a framework that can be easily used for defining strategies and organising the supply chain according to the different levels of luxury.Originality/valueThis study represents an important evolution in organising the current literature on luxury supply chain management into a framework that covers critical success factors, supply chain performance goals and configuration elements for three different levels of luxury, which in turn creates promising opportunities for future enquiry.
Does brand type affect what consumers discuss? A comparison of attribute-based reviews of value and premium brands of an innovative product
Authors: Yi Jisu , Oh Yun Kyung 
Published: July 2021

PurposeThis research aims to investigate the role of brand types (value brand vs premium brand) in determining review content. Specifically, this research focuses on attribute-based reviews for an innovative product and suggests that consumers of value brands tend to discuss more attributes in their product reviews than those of premium brands. Also, this research suggests that review valence and time have moderating effects on the relationship between brand types and the number of attributes discussed in a review.Design/methodology/approachThis research employed a data set of online consumer reviews (N = 106,980) for wireless earbuds from Amazon.com. It extracted products' attributes from review text using Bigram analysis and measured the number of attributes discussed in a review. It then analyzed the effect of brand types (value brand vs premium brand) on the number of attributes and the moderating effect of review valence and time.FindingsThe estimation results of Poisson models reveal that reviews for value brands tend to contain more product attributes than reviews for premium brands. Interestingly, the tendency is stronger among positive (vs negative) reviews, and it decreases over time as more reviews are accumulated.Originality/valueWhile existing studies focused on the outcomes of the review content, there was not enough investigation into the determinants of the review content. This study focuses on the number of attributes discussed in a review, a key content characteristic, and provides the first empirical evidence that review content differs by brand types of an innovative product.
Recommended angle of a modular dynamic façade in hot-arid climate: daylighting and energy simulation
Authors: Golzan Seyedeh Samaneh , Pouyanmehr Mina , Sadeghi Naeini Hassan 
Published: July 2021

PurposeThe modular dynamic façade (MDF) concept could be an approach in a comfort-centric design through proper integration with energy-efficient buildings. This study focuses on obtaining and/or calculating an efficient angle of the MDF, which would lead to the optimum performance in daylight availability and energy consumption in a single south-faced official space located in the hot-arid climate of Yazd, Iran.Design/methodology/approachThe methodology consists of three fundamental parts: (1) based on previous related studies, a diamond-based dynamic skin façade was applied to a south-faced office building in a hot-arid climate; (2) the daylighting and energy performance of the model were simulated annually; and (3) the data obtained from the simulation were compared to reach the optimum angle of the MDF.FindingsThe results showed that when the angle of the MDF openings was set at 30°, it could decrease energy consumption by 41.32% annually, while daylight simulation pointed that the space experienced the minimum possible glare at this angle. Therefore, the angle of 30° was established as the optimum angle, which could be the basis for future investment in responsive building envelopes.Originality/valueThis angular study simultaneously assesses the daylight availability, visual comfort and energy consumption on a MDF in a hot-arid climate.
Indígena or Não‐Indígena? The Gendered Politics of Assimilation in Colonial Mozambique, 1917–61
Authors: Havstad Lilly 
Published: July 2021

AbstractThis article examines the intersections of race, sex and gender that shaped the legal system of assimilation in colonial Mozambique. Between 1917 and 1961, Africans could apply for assimilated (assimilado/a) status, granting them limited rights of Portuguese citizenship. Analysis of assimilation case files, administrative reports and public debates in local newspapers reveals that assimilation was an ambiguous, changing and gendered system shaped not only by its Portuguese legislative architects, but also by the African men and women who navigated the law, and encouraged its reforms, over time. Using a feminist historical approach to challenge entrenched scholarly interpretations of the system as uniformly male gendered, this article reveals that marriage, miscegenation and women were at the centre of the assimilation system as a contested site of the politics of ‘civilisation’ in the colonial capital of Lourenço Marques.