bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urinary Tract Infection Bacterial at RSUP H. Adam Malik Medan in 2019: an Overview Study
Authors: Teguh Firdaus  , Rina Yunita  
Published: December 2021

Urinary Tract Infection (UTI) is the second-largest infection after respiratory tract infection (WHO, 2013). The prevalence of UTI in Indonesia is relatively high. UTI originated from the invasion and proliferation of microorganisms into the urinary tract in meaningful quantities. Gram-negative bacteria are mostly the cause of UTI including E. coli, Klebsiella sp, Enterobacter sp, and Proteus sp. Patients with UTI with inadequate treatment can experience complications e.g., acute renal failure and urosepsis. This study aims to find out the profile of bacteria that cause UTI at RSUP H. Adam Malik Medan in 2019. This descriptive study is using a non-analytical retrospective approach conducted to secondary data from urinary culture examination at RSUP H. Adam Malik Medan. Data were analyzed using the SPSS 24. Gram-negative bacteria results of 72.7% with E. coli postulated as the most common etiology (33,3%) and gram-positive bacteria results of 27.3%, dominated by Enterococcus faecalis (16.3%). The sensitivity study exhibited various results e.g., 100%, 98.2%, and 97.0% in E. coli for Tigecycline, Meropenem, and Amikacin respectively. Enterococcus faecalis also posed a similar results regarding its sensitivity result to different antibiotics. Gram-negative bacteria are the most common bacterial etiology of UTI, specifically E. coli with comparable sensitivity results
Charasteristics of COVID-19 Patients Treated At Santa Elisabeth Medan Hospital 2020
Authors: Triputri Nency , Noni Novisari Soeroso  
Published: December 2021

Corona Virus Disease (COVID-19) is a disease caused by a new type of coronavirus (SARS-CoV-2). This virus was first discovered in Wuhan, China for the first time and has infected 90,308 people as of March 2, 2020. Infection is transmitted by large droplets or droplets within 1 - 2 m, which are generated during coughing and sneezing by symptomatic patients and people without symptoms. This infection is acquired by inhaling these droplets or touching a contaminated surface and then touching the nose, mouth and eyes. To see the characteristics of COVID-19 patients who are treated at the Santa Elisabeth Hospital Medan in 2020. Type of research is a descriptive study with a cross-sectional study design with a retrospective approach using total sampling technique. This type of research data is secondary data obtained from the medical record data of positive COVID-19 patients confirmed to be hospitalized in several Medan city hospitals in 2020.Of the 230 study samples, 147 confirmed positive COVID-19 patients were found, dominated by the age range 46-55 years. The sex most common is male. The most common clinical symptoms are respiratory disorders such as coughing, shortness of breath and fever.The number of confirmed positive patients at the hospital. Santa Elisabeth in March to October 2020 is 147 people (51.1%).
LENGTH-WEIGHT RELATIONSHIP AND REPRODUCTION OF SULPHUR GOATFISH Upeneus sulphureus (Cuvier, 1829) AT PPI KALIANDA, SOUTH LAMPUNG
Authors: Pradana M Aldhy Nur , Damai Abdullah Aman , Yudha Indra Gumay , Suparmono Suparmono 
Published: December 2021

Sulphur goatfish (Upeneus sulphureus) is the dominant caught with a fairly high level of utilization. This research aimed to analyze aspects of growth and reproduction sulphur goatfish caught by rampus nets landed at the fishing port of PPI Kalianda. Growth aspects that were observed included the length-weight, while the reproductive aspects observed is the sex ratio, gonad maturity level, and the size of sulphur goatfish at first maturity. This research was conducted in May-July 202 with the research method used simple random sampling method. The fish samples obtained during the study were 600 individuals, the results showed that Sulphur goatfish had negative allometric growth. The equation for the long-weight relationship of sulphur goatfish is log W = -4,6 + 2,9 log L or W = 2,8x10-5L2,9. The sex ratio of sulphur goatfish in May and July were not 1: 1, while the ideal condition was achieved in June. The gonad maturity level distribution of sulphur goatfish was dominated by TKG III and IV for female, while male sulphur goatfish was dominated by TKG II and III. Size at first maturity of sulphur goatfish females was 169,1 ± 1,03mm.
Peculiarities of knee replacement in patients with rheumatoid arthritis
Authors: Volchenko D. V. , Akhtyamov I. F. , Lapshina S. A. , Gilmutdinov I. Sh. 
Published: December 2021

Introduction. Rheumatoid arthritis (RA), as a steadily progressive disease leading to joint destruction and functional instability of the knee joints, often requires orthopedic correction.The aim. Of the review was to analyze the surgery strategy and technical features of knee arthroplasty and replacement in patients with RA.Basic provisions. RA is characterized by a number of abnormalities not only due to erosive arthritis and active persistent synovitis, but also caused by a decrease in bone mineral density, damage to the periarticular structures and patella, the formation of bone defects, as well as a high risk of postoperative complications. All these factors should be taken into account while choosing relevant surgical treatment and knee replacement technique. The article presents the optimal approaches for performing knee replacement in RA patients, taking into account the peculiarities of the existing structural and functional disorders.Conclusion. TEC in patients with RA requires a personalized approach based on evaluation of disease-associated risk factors affecting the results of orthopedic correction and the likelihood of complications.
Ankylosing spondylitis in combination with Crohn's disease: clinical case
Authors: Tarasova D. D. , Shilova L. N. , Koroleva M. V. 
Published: December 2021

Introduction. Ankylosing spondylitis and Crohn's disease are chronic recurrent autoimmune diseases. In case of a combined course their activity tends to progress, regardless of the phase of the underlying disease.The aim of the study. To analyze the combination of ankylosing spondylitis and Crohn's disease, issues of its diagnosis and selection of therapy.Results. The combination of ankylosing spondylitis and Crohn's disease tend to progress independently, regardless of the phase of the underlying disease. The main problem that complicates diagnosis is the lack of a unifed approach to the defnition of extra skeletal and extraintestinal manifestations.Conclusion. The management of patients with a combination of ankylosing spondylitis and Crohn's disease is a common problem of rheumatologists and gastroenterologists. An interdisciplinary approach will allow timely diagnosis of extra-skeletal and extra-intestinal manifestations and correct therapy.
Redox Potentials of Disulfide Bonds in LOXL2 Studied by Nonequilibrium Alchemical Simulation
Authors: Lin Lirui , Zou Haiying , Li Wenjin , Xu Li-Yan , Li En-Min , Dong Geng 
Published: December 2021

Lysyl oxidase-like 2 (LOXL2) is a metalloenzyme that catalyzes the oxidative deamination ε-amino group of lysine. It is found that LOXL2 is a promotor for the metastasis and invasion of cancer cells. Disulfide bonds are important components in LOXL2, and they play a stabilizing role for protein structure or a functional role for regulating protein bioactivity. The redox potential of disulfide bond is one important property to determine the functional role of disulfide bond. In this study, we have calculated the reduction potential of all the disulfide bonds in LOXL2 by non-equilibrium alchemical simulations. Our results show that seven of seventeen disulfide bonds have high redox potentials between −182 and −298 mV and could have a functional role, viz., Cys573–Cys625, Cys579–Cys695, Cys657–Cys673, and Cys663–Cys685 in the catalytic domain, Cys351–Cys414, Cys464–Cys530, and Cys477–Cys543 in the scavenger receptor cysteine-rich (SRCR) domains. The disulfide bond of Cys351–Cys414 is predicted to play an allosteric function role, which could affect the metastasis and invasion of cancer cells. Other functional bonds have a catalytic role related to enzyme activity. The rest of disulfide bonds are predicted to play a structural role. Our study provides an important insight for the classification of disulfide bonds in LOXL2 and can be utilized for the drug design that targets the cysteine residues in LOXL2.
Robust Rank Aggregation and Least Absolute Shrinkage and Selection Operator Analysis of Novel Gene Signatures in Dilated Cardiomyopathy
Authors: Ma Xiao , Mo Changhua , Huang Liangzhao , Cao Peidong , Shen Louyi , Gui Chun 
Published: December 2021

Objective: Dilated cardiomyopathy (DCM) is a heart disease with high mortality characterized by progressive cardiac dilation and myocardial contractility reduction. The molecular signature of dilated cardiomyopathy remains to be defined. Hence, seeking potential biomarkers and therapeutic of DCM is urgent and necessary.Methods: In this study, we utilized the Robust Rank Aggregation (RRA) method to integrate four eligible DCM microarray datasets from the GEO and identified a set of significant differentially expressed genes (DEGs) between dilated cardiomyopathy and non-heart failure. Moreover, LASSO analysis was carried out to clarify the diagnostic and DCM clinical features of these genes and identify dilated cardiomyopathy derived diagnostic signatures (DCMDDS).Results: A total of 117 DEGs were identified across the four microarrays. Furthermore, GO analysis demonstrated that these DEGs were mainly enriched in the regulation of inflammatory response, the humoral immune response, the regulation of blood pressure and collagen–containing extracellular matrix. In addition, KEGG analysis revealed that DEGs were mainly enriched in diverse infected signaling pathways. Moreover, Gene set enrichment analysis revealed that immune and inflammatory biological processes such as adaptive immune response, cellular response to interferon and cardiac muscle contraction, dilated cardiomyopathy are significantly enriched in DCM. Moreover, Least absolute shrinkage and selection operator (LASSO) analyses of the 18 DCM-related genes developed a 7-gene signature predictive of DCM. This signature included ANKRD1, COL1A1, MYH6, PERELP, PRKACA, CDKN1A, and OMD. Interestingly, five of these seven genes have a correlation with left ventricular ejection fraction (LVEF) in DCM patients.Conclusion: Our present study demonstrated that the signatures could be robust tools for predicting DCM in clinical practice. And may also be potential treatment targets for clinical implication in the future.
Association Between Out-of-Hour Admission and Short- and Long-Term Mortality in Acute Myocardial Infarction: A Systematic Review and Meta-Analysis
Authors: Yu Yue-Yan , Zhao Bo-Wen , Ma Lan , Dai Xiao-Ce 
Published: December 2021

Objectives: Out-of-hour admission (on weekends, holidays, and weekday nights) has been associated with higher mortality in patients with acute myocardial infarction (AMI). We conducted a meta-analysis to verify the association between out-of-hour admission and mortality (both short- and long-term) in AMI patients.Design: This Systematic review and meta-analysis of cohort studies.Data Sources: PubMed and EMBASE were searched from inception to 27 May 2021.Eligibility Criteria for Selected Studies: Studies of any design examined the potential association between out-of-hour admission and mortality in AMI.Data Extraction and Synthesis: In total, 2 investigators extracted the data and evaluated the risk of bias. Analysis was conducted using a random-effects model. The results are shown as odds ratios [ORs] with 95% confidence intervals (CIs). I2 value was used to estimate heterogeneity. Grading of Recommendations Assessment, Development, and Evaluation was used to assess the certainty of the evidence.Results: The final analysis included 45 articles and 15,346,544 patients. Short-term mortality (defined as either in-hospital or 30-day mortality) was reported in 42 articles (15,340,220 patients). Out-of-hour admission was associated with higher short-term mortality (OR 1.04; 95%CI 1.02–1.05; I2 = 69.2%) but there was a significant statistical indication for publication bias (modified Macaskill's test P &lt; 0.001). One-year mortality was reported in 10 articles (1,386,837 patients). Out-of-hour admission was also associated with significantly increased long-term mortality (OR 1.03; 95%CI 1.01–1.04; I2 = 66.6%), with no statistical indication of publication bias (p = 0.207). In the exploratory subgroup analysis, the intervention effect for short-term mortality was pronounced among patients in different regions (p = 0.04 for interaction) and socio-economic levels (p = 0.007 for interaction) and long-term mortality was pronounced among patients with different type of AMI (p = 0.0008 for interaction) or on different types of out-to-hour admission (p = 0.006 for interaction).Conclusion: Out-of-hour admission may be associated with an increased risk of both short- and long-term mortality in AMI patients.Trial Registration: PROSPERO (CRD42020182364).
Frequency-Domain Characteristics Response to Passive Exercise in Patients With Coronary Artery Disease
Authors: Zhang Xiaodong , Zhang Yahui 
Published: December 2021

Purpose: The enhanced external counterpulsation (EECP), a kind of passive exercise, is a novel non-invasive therapy used to improve peripheral perfusion in patients with coronary artery disease (CAD). However, whether frequency-domain characteristics of peripheral hemodynamics may benefit from passive exercise needs to be verified.Methods: We recruited 21 patients with CAD and 21 healthy controls in this study. Ultrasonic blood flow velocity spectrum in left carotid (LC) and right carotid (RC) common arteries, and right brachial (RB) and right femoral (RF) arteries was monitored using an ultrasonic Doppler. Frequency-domain characteristics before, during, and after passive exercise were extracted from ultrasonic spectrum images. The first and second peak amplitudes/frequencies (y1, y2, x1, x2) and power spectral energy ratio (PSER) in the 0–2.05 Hz/0.87 Hz (p5, p6) were calculated by fast Fourier transform and power spectrum density analysis.Results: For the amplitude and frequency characteristics of the spectrum, y1 in the LC of patients with CAD was significantly decreased during exercise (p = 0.036), whereas, y2 was significantly decreased immediately after passive exercise (p = 0.038). Besides those, y1 only in the RC and RB of controls was significantly decreased during exercise. Immediately after exercise, y2 in the LC of control was significantly lower than at the baseline (p = 0.014). For the energy ratio characteristics of the spectrum, there was an opposite response in the two groups that p6 was significantly reduced and elevated in the LC of controls and in the RB of patients with CAD during exercise (both p &lt; 0.05).Conclusions: Passive exercise reduces amplitude and frequency characteristics of carotid arteries, while there was an opposite response of energy ratio characteristics in the LC and RB arteries to passive exercise between CAD patients and controls. Additionally, energy ratio characteristics of spectrum in the brachial artery were markedly elevated in CAD patients during passive exercise. Moreover, passive exercise only reduces amplitude characteristics of LC artery in the control group.
Altered Cortisol Metabolism Increases Nocturnal Cortisol Bioavailability in Prepubertal Children With Type 1 Diabetes Mellitus
Authors: Brossaud Julie , Corcuff Jean-Benoît , Vautier Vanessa , Bergeron Aude , Valade Aurelie , Lienhardt Anne , Moisan Marie-Pierre , Barat Pascal 
Published: December 2021

ObjectiveDisturbances in the activity of the hypothalamus-pituitary-adrenal axis could lead to functional alterations in the brain of diabetes patients. In a later perspective of investigating the link between the activity of the hypothalamus-pituitary-adrenal axis and the developing brain in children with diabetes, we assessed here nocturnal cortisol metabolism in prepubertal children with type 1 diabetes mellitus (T1DM).MethodsPrepubertal patients (aged 6–12 years) diagnosed with T1DM at least 1 year previously were recruited, along with matched controls. Nocturnal urine samples were collected, with saliva samples taken at awakening and 30 minutes after awakening. All samples were collected at home over 5 consecutive days with no detectable nocturnal hypoglycaemia. The State-Trait Anxiety Inventory (trait scale only) and Child Depression Inventory were also completed. Glucocorticoid metabolites in the urine, salivary cortisol (sF) and cortisone (sE) were measured by liquid chromatography–tandem mass spectrometry. Metabolic data were analysed by logistic regression, adjusting for sex, age, BMI and trait anxiety score.ResultsUrine glucocorticoid metabolites were significantly lower in T1DM patients compared to controls. 11β-hydroxysteroid dehydrogenase type 1 activity was significantly higher, while 11β-hydroxysteroid dehydrogenase type 2, 5(α+β)-reductase and 5α-reductase levels were all lower, in T1DM patients compared to controls. There was a significant group difference in delta sE level but not in delta sF level between the time of awakening and 30 minutes thereafter.ConclusionsOur findings suggest that altered nocturnal cortisol metabolism and morning HPA axis hyperactivity in children with T1DM leads to greater cortisol bioavailability and lower cortisol production as a compensatory effect. This altered nocturnal glucocorticoid metabolism when cortisol production is physiologically reduced and this HPA axis hyperactivity question their impact on brain functioning.
Haplotype-Based Noninvasive Prenatal Diagnosis of 21 Families With Duchenne Muscular Dystrophy: Real-World Clinical Data in China
Authors: Kong Lingrong , Li Shaojun , Zhao Zhenhua , Feng Jun , Chen Guangquan , Liu Lina , Tang Weiqin , Li Suqing , Li Feifei , Han Xiujuan , Wu Di , Zhang Haichuan , Sun Luming , Kong Xiangdong 
Published: December 2021

Noninvasive prenatal diagnosis (NIPD) of single-gene disorders has recently become the focus of clinical laboratories. However, reports on the clinical application of NIPD of Duchenne muscular dystrophy (DMD) are limited. This study aimed to evaluate the detection performance of haplotype-based NIPD of DMD in a real clinical environment. Twenty-one DMD families at 7–12 weeks of gestation were prospectively recruited. DNA libraries of cell-free DNA from the pregnant and genomic DNA from family members were captured using a custom assay for the enrichment of DMD gene exons and spanning single-nucleotide polymorphisms, followed by next-generation sequencing. Parental haplotype phasing was based on family linkage analysis, and fetal genotyping was inferred using the Bayes factor through target maternal plasma sequencing. Finally, the entire experimental process was promoted in the local clinical laboratory. We recruited 13 complete families, 6 families without paternal samples, and 2 families without probands in which daughter samples were collected. Two different maternal haplotypes were constructed based on family members in all 21 pedigrees at as early as 7 gestational weeks. Among the included families, the fetal genotypes of 20 families were identified at the first blood collection, and a second blood collection was performed for another family due to low fetal concentration. The NIPD result of each family was reported within 1 week. The fetal fraction in maternal cfDNA ranged from 1.87 to 11.68%. In addition, recombination events were assessed in two fetuses. All NIPD results were concordant with the findings of invasive prenatal diagnosis (chorionic villus sampling or amniocentesis). Exon capture and haplotype-based NIPD of DMD are regularly used for DMD genetic diagnosis, carrier screening, and noninvasive prenatal diagnosis in the clinic. Our method, haplotype-based early screening for DMD fetal genotyping via cfDNA sequencing, has high feasibility and accuracy, a short turnaround time, and is inexpensive in a real clinical environment.
Treatment With Cladribine Selects IFNγ+IL17+ T Cells in RRMS Patients – An In Vitro Study
Authors: Dobreanu Minodora , Manu Doina Ramona , Mănescu Ion Bogdan , Gabor Manuela Rozalia , Huţanu Adina , Bărcuţean Laura , Bălaşa Rodica 
Published: December 2021

BackgroundMultiple sclerosis (MS) is an incurable autoimmune disease mediated by a heterogeneous T cell population (CD3+CD161+CXCR3−CCR6+IFNγ−IL17+, CD3+CXCR3+CCR6+IFNγ+IL17+, and CD3+CXCR3+IFNγ+IL17− phenotypes) that infiltrates the central nervous system, eliciting local inflammation, demyelination and neurodegeneration. Cladribine is a lymphocyte-depleting deoxyadenosine analogue recently introduced for MS therapy as a Disease Modifying Drug (DMD). Our aim was to establish a method for the early identification and prediction of cladribine responsiveness among MS patients.MethodsAn experimental model was designed to study the cytotoxic and immunomodulatory effect of cladribine. T cell subsets of naïve relapsing-remitting MS (RRMS) patients were analyzed ex vivo and in vitro comparatively to healthy controls (HC). Surviving cells were stimulated with rh-interleukin-2 for up to 14days. Cell proliferation and immunophenotype changes were analyzed after maximal (phorbol myristate acetate/ionomycin/monensin) and physiological T-cell receptor (CD3/CD28) activation, using multiparametric flow cytometry and xMAP technology.ResultsEx vivo CD161+Th17 cells were increased in RRMS patients. Ex vivo to in vitro phenotype shifts included: decreased CD3+CCR6+ and CD3+CD161+ in all subjects and increased CD3+CXCR3+ in RRMS patients only; Th17.1 showed increased proliferation vs Th17 in all subjects; CD3+IL17+ and CD3+IFNγ+IL17+ continued to proliferate till day 14, CD3+IFNγ+ only till day 7. Regarding cladribine exposure: RRMS CD3+ cells were more resistant compared to HC; treated CD3+ cells proliferated continuously for up to 14 days, while untreated cells only up to 7 days; both HC/RRMS CD3+CXCR3+ populations increased from baseline till day 14; in RRMS patients vs HC, IL17 secretion from cladribine-treated cells increased significantly, in line with the observed proliferation of CD3+IL17+ and CD3+IFNγ+IL17+ cells; in both HC/RRMS, cladribine led to a significant increase in CD3+IFNγ+ cells at day 7 only, having no further effect at day14. IFNγ and IL17 secreted in culture media decreased significantly from ex vivo to in vitro.ConclusionsCD3+ subtypes showed different responsiveness due to selectivity of cladribine action, in most patients leading to in vitro survival/proliferation of lymphocyte subsets known as pathogenic in MS. This in vitro experimental model is a promising tool for the prediction of individual responsiveness of MS patients to cladribine and other DMDs.