bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and culture-based assessment of the microbiome in a zebrafish (Danio rerio) housing system during set-up and equilibration
Authors: Ericsson Aaron C. , Busi Susheel B. , Davis Daniel J. , Nabli Henda , Eckhoff David C. , Dorfmeyer Rebecca A. , Turner Giedre , Oswalt Payton S. , Crim Marcus J. , Bryda Elizabeth C. 
Published: August 2021

Abstract
Background
Zebrafish used in research settings are often housed in recirculating aquaculture systems (RAS) which rely on the system microbiome, typically enriched in a biofiltration substrate, to remove the harmful ammonia generated by fish via oxidation. Commercial RAS must be allowed to equilibrate following installation, before fish can be introduced. There is little information available regarding the bacterial community structure in commercial zebrafish housing systems, or the time-point at which the system or biofilter reaches a microbiological equilibrium in RAS in general.

Methods
A zebrafish housing system was monitored at multiple different system sites including tank water in six different tanks, pre- and post-particulate filter water, the fluidized bed biofilter substrate, post-carbon filter water, and water leaving the ultra-violet (UV) disinfection unit and entering the tanks. All of these samples were collected in quadruplicate, from prior to population of the system with zebrafish through 18 weeks post-population, and analyzed using both 16S rRNA amplicon sequencing and culture using multiple agars and annotation of isolates via matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. Sequencing data were analyzed using traditional methods, network analyses of longitudinal data, and integration of culture and sequence data.

Results
The water microbiome, dominated by Cutibacterium and Staphylococcus spp., reached a relatively stable richness and composition by approximately three to four weeks post-population, but continued to evolve in composition throughout the study duration. The microbiomes of the fluidized bed biofilter and water leaving the UV disinfection unit were distinct from water at all other sites. Core taxa detected using molecular methods comprised 36 amplicon sequence variants, 15 of which represented Proteobacteria including multiple members of the families Burkholderiaceae and Sphingomonadaceae. Culture-based screening yielded 36 distinct isolates, and showed moderate agreement with sequencing data.

Conclusions
The microbiome of commercial RAS used for research zebrafish reaches a relatively stable state by four weeks post-population and would be expected to be suitable for experimental use following that time-point.
The hidden crisis: COVID-19 and impact on mental health of medical students in Pakistan
Authors: Imran Nazish , Haider Imran Ijaz , Mustafa Ali Burhan , Aamer Irum , Kamal Zahid , Rasool Ghulam , Azeem Muhammad Waqar , Javed Afzal 
Published: August 2021

Abstract
Background
Medical students have faced an enormous disruption to their lives due to the COVID-19 pandemic. The study aimed to assess the impact of COVID-19 on medical student’s psychological well-being in Pakistan. Following ethical approval, an online survey developed in collaboration with World Psychiatric Association (WPA) was distributed among medical students of 5 Medical colleges in the Punjab province of Pakistan between August and September 2020. Patient Health Questionnaire (PHQ-9), Generalized Anxiety Disorder Scale (GAD-7), and Risk Assessment Suicidality Scale (RASS) were used to assess psychological well-being. Data was analyzed using SPSS 26.0.

Results
Eleven hundred medical students responded, 756 (69%) being females. More than 2/3rd admitted that their emotional state got worse in relation to appearance of anxiety, insecurity, and sadness, compared to before the outbreak of COVID-19. Prevalence of anxiety and depressive symptoms were 48.6% and 48.1%, respectively. Female medical students, pre-clinical students, and those with a previous psychiatric history reported experiencing more anxiety and depression symptoms (P value < 0.001). One in five medical students thought that it would be better if they were dead, and 8% admitted to often think of committing suicide during the past 2 weeks. RASS and subscales (intention, life, and history) scores were higher in females and students with previous psychiatric problems.

Conclusion
Our findings underscore that the impact of COVID-19 on medical students has been significant; hence, it is crucial for medical colleges to employ strategies to maintain the student’s well-being with safeguards like reassurance, support, and confidential student-centered psychiatric services. The use of virtual platforms (websites, email) to educate and screen students by staff members can create a positive impact. The limitations of this study include cross-sectional design, the possibility of selective participation being web-based survey, response bias, and the possibility of reluctance of students to report mental health problems due to stigma.
Financial Impact of an Automated Oncology Dose-Rounding Initiative: One-Year Analysis
Authors: Dela-Pena Jacqueline C. , Eschenburg Katie A. , LaRocca Vincent W. , Patel Dipale , Hough Shannon M. 
Published: August 2021

PURPOSE Infusion drugs are regarded as one of the high-cost health care expenditures. One approach to decrease drug expenditures is by dose-rounding biologics and cytotoxic agents. The Hematology/Oncology Pharmacy Association recommends that biologic and cytotoxic agents are rounded to the nearest vial size if they are within 10% of the ordered dose. The purpose of this initiative is to determine the impact of an automated dose-rounding algorithm on drug expenses. METHODS The dose-rounding algorithm was developed and integrated into the computerized physician order entry system for automated dose rounding to minimize impact on current workflow and to reduce medication errors. Twenty-four medications were preselected for dose rounding and included in the analysis. Ordered doses were automatically rounded to the nearest vial size if the dose was within 10% of the original dose. Prescribers then either reviewed and signed the rounded dose or manually entered the nonrounded dose. Cost savings were calculated as drug expense savings from doses rounded down. RESULTS From July 2018 to June 2019, 10,206 doses of the selected medications were administered. Dose rounding occurred in 5,069 doses (49.7%). All 24 medications within the initiative were administered within the time of analysis. Of the rounded doses administered, 2,516 (49.6%) were rounded down to a commercially available vial size. Using wholesale acquisition cost pricing, the drug expense savings was approximately $3.6 million US dollars (USD). The medications with the highest savings were trastuzumab and ipilimumab, with annual savings of $756,780 USD and $494,517 USD, respectively. CONCLUSION The automated dose-rounding algorithm at Michigan Medicine reduced drug expenditures substantially, and its integration within the computerized physician order entry system had minimal impact on current workflow.
Ausgrabungen in der Alten Stadtgärtnerei, Elsässerstrasse 2a (St. Johanns-Park)
Authors: Aebi Thomas , D'Aujourd'hui Rolf , Etter Hansueli F. 
Published: August 2021

Die erste Etappe des «Grünparks St. Johann», die 1988/89 auf dem Areal der Alten Stadtgärtnerei realisiert wurde, führte zu Bodenabsenkungen im Bereich des ehemaligen Spitalfriedhofs St. Johann. Dabei mussten rund 1000 Gräber des neuzeitlichen Friedhofs unter schwierigen Bedingungen in nur fünf Monaten freigelegt, untersucht und geborgen werden.
Einen Spitalfriedhof aus dem 19. Jahrhundert archäologisch und anthropologisch zu untersuchen ist für Europa bislang einmalig. Es bot sich uns hier die Gelegenheit, die knöchernen Reste von Menschen aus der Frühzeit der Industrialisierung zu bearbeiten, über die wir aus dem Spitalarchiv von damals noch individuelle und medizinische Daten zu finden hofften. Dazu kam der wichtige Umstand, dass die Belegungszeit nur etwa eine Generation betrug, wodurch eine anthropologische Momentaufnahme möglich werden sollte.
In der östlichen Hälfte der Grabungsfläche kamen unter dem Skeletthorizont die Fundamente zweier Steinbauten zum Vorschein. Der Abbruchschutt eines der Gebäude enthielt zahlreiche Funde aus dem 13. Jahrhundert.
Steinengraben 22/Leonhardsstrasse 22/24: Zum Neufund der spätmittelalterlichen Kontermauer
Authors: Matt Christoph Ph. 
Published: August 2021

An der Ecke Steinengraben 20/Leonhardsstrasse 22/24 stiess man auf die auf der gegenüberliegenden Seite des Stadtgrabens errichtete Gegenmauer oder Kontereskarpe zur Äusseren Stadtmauer, die bis in rund 7 m Tiefe reichte und unverputzt war. Die auf einer Länge von mehr als 40 m über eine Höhe von 2 bis 4,3 m freigelegte Rückseite der Mauer wurde untersucht und dokumentiert.
Rheingasse 86/0berer Rheinweg 81 (1989/4): Aufschlüsse zur Baugeschichte eines Kleinbasler Hauses
Authors: Matt Christoph Ph. 
Published: August 2021

Seit jeher lag die Parzelle zwar am Stadtrand bzw. am Rheinufer, doch während sie bis zum Abbruch der rheinseitigen Kleinbasler Stadtmauer im Jahre 1863 nur von der Landseite her erschlossen war, war sie danach von zwei Gassen her zugänglich.
Im Innern des Hinterhauses konnte bei den Maueruntersuchungen mit grosser Wahrscheinlichkeit die mittelalterliche Stadtmauer nachgewiesen werden. Zudem werden im Bericht die bei den Untersuchungen registrierten Befunde vorgestellt, soweit sie für die Baugeschichte aus der Zeit vor dem Neubau dieser Liegenschaft (1824/25) von Belang sind.
Gerbergässlein 2 (1989/6): Zur Baugeschichte der Häuser «zum Schwarzen Turm» und «zum Grünen Stern» sowie Reste einer Gerberei aus dem 18./19. Jh.
Authors: Matt Christoph Ph. 
Published: August 2021

Anlässlich einer Totalrenovierung der verschiedenen Gebäudeteile 1989/1990 konnten baugeschichtliche und archäologische Untersuchungen in der Liegenschaft vorgenommen werden. Aufgrund der komplizierten Aufteilung dieser Liegenschaft war schon zu Beginn der Untersuchungen mit einer komplexen Baugeschichte zu rechnen.
Insbesondere konnte der aufgrund des Hausnamens zum Schwarzen Turm und der erstaunlich dicken Hinterhausmauern hier vermuteten Turm nachgewiesen werden. Zudem wurden Erkenntnisse zur Baugeschichte der mittelalterlichen Vorderhäuser sowie zum schon seit dem späten Mittelalter überlieferten Gerbereihandwerk gewonnen. Die freigelegten Gerberbottiche konnten ins 18./19. Jahrundert datiert werden.
Die Untersuchungen im Stadtmauerturm, Petersgraben 43
Authors: Jaggi Bernard 
Published: August 2021

Geht man davon aus, dass die erste Ausstattung des Türmchens mit Wandmalereien, die in die Zeit von 1300 bis spätestens 1350 datieren dürften, aus der Bauzeit des Turmes stammt, so ist die Frage nach dessen Funktion eigentlich beantwortet. Es kann sich bei diesem Bauwerk nicht um einen «echten» Stadtmauerturm handeln, sondern es muss als wehrhaft erscheinendes Gartenhaus des Zerkindenhofs betrachtet werden. Es gibt keine Anhaltspunkte dafür, dass es sich bereits um eine Umbauversion handelt.
Der Befund legt die Vermutung nahe, dass in der Zeit kurz nach 1300 der Turm als geschlossenes Gehäuse (mit unbekannter Überdachung) an die Innere Stadtmauer (von ca. 1250) angebaut wurde . Der Turm schloss über die gesamte Höhe an die Stadtmauer an, d.h. die erhaltene Turmhöhe gibt auch einen Hinweis auf die Mindesthöhe der Inneren Stadtmauer.
Falknerstrasse 7 / Freie Strasse 32, 1990/14: Mittelalterliche Areal- und Gebäudemauern
Authors: Matt Christoph Ph. , Bing Christian 
Published: August 2021

Die Parzelle Nr. 32, auf der die heutige Liegenschaft steht, bestand bis zum Ende des 19. Jahrhunderts aus zwei schmalen langgezogenen Parzellen, die von der Freien Strasse bis zum damals noch offenen Birsig reichten.
Die im neu angelegten Keller aufgefundenen Mauern sind als letzte Zeugen einer Hinterhofüberbauung zu betrachten, die im Laufe des späten Mittelalters entstanden und im frühen 17. Jahrhundert, zur Zeit der Zeichnung der Merianschen Vogelschaupläne, bereits wieder abgerissen waren.
Murus Gallicus: Vorbericht zu den Grabungen 1990 bis 1992
Authors: Deschler-Erb Eckhard , Richner Kaspar 
Published: August 2021

Die spätkeltische Befestigung auf dem Basler Münsterhügel, der sogenannte Murus Gallicus, wurde 1971 beim Neubau der Turnhalle an der Rittergasse entdeckt. 1976 und 1979 fanden weitere Grabungen im Bereich des Murus Gallicus statt. Nach einer mehr als zehnjährigen Pause konnten 1990 die Untersuchungen am keltischen Wall wieder aufgenommen werden, als im Zuge der Renovation der Gerichtsliegenschaften an der Bäumleingasse im Hinterhof von Bäumleingasse Nr. 3-5 eine Garage in den Hang hinein gebaut wurde.
Im Jubiläumsjahr der Schweizerischen Eidgenossenschaft 1991 erhielt der Kantonsarchäologe den Auftrag, eine historische Ausstellung im öffentlichen Raum zu realisieren. Als Thema dieser Ausstellung wurde die keltische Befestigung auf dem Münsterhügel gewählt. 1991 fanden gezielte Sondierungen im Hof des ehemaligen Rittergasse-Schulhauses statt, um einerseits Wallstücke zu lokalisieren, die sich für eine Konservierung eignen würden, und andererseits verschiedene wissenschaftliche Fragen zu klären, die sich beim Vergleich der Ergebnisse der Grabung von 1990 mit den Interpretationen früherer Grabungen ergeben hatten. 1992 wurde mit dem Bau von drei Schutzbauten - Kellern mit Glasdächern - begonnen, in denen Teile des keltischen Walles für das Publikum sichtbar gemacht und erläutert werden.
Nebst dem Murus Gallicus konnten der zugehörige Graben, keltische Siedlungsspuren vor dem Wall, römische Siedlungs- und Schuttschichten, ein frühmittelalterlicher Friedhof, mittelalterliche Aufschüttungen, Fundamente der heute abgebrochenen spätmittelalterlichen/frühneuzeitlichen Überbauung und zugehörige Gartenniveaus beobachtet werden.
Riehen, Wenkenköpfli (Reservoir), 1992/11
Authors: Leuzinger Urs 
Published: August 2021

Auf dem Wenkenköpfli wurde östlich des Reservoirs eine kleine neolithische Freilandstation entdeckt. Die geschützte Lage der bewaldeten Anhöhe und die nahen Quellen haben sich wohl ausgezeichnet für eine Besiedlung geeignet.
Bis jetzt fanden sich an der Oberfläche 16 Steinartefakte sowie zwei prähistorische Keramikfragmente. Insgesamt fünf Silices sind sekundär modifiziert worden.
Gemsberg 2/4, Restaurant Löwenzorn, 1992/33
Authors: Matt Christoph Ph. , Bing Christian , Moor Beatrice 
Published: August 2021

Eine vor dem Umbau im Restaurant Löwenzorn durchgeführte Sondierung erbrachte in einem Sondierschnitt interessante mittelalterliche Funde und Befunde.
Als besonderer Fund ist eine Meermuschel zu werten, wie sie in mittelalterlichem Fundzusammenhang nur höchst selten anzutreffen ist: ein sog . Tritonshorn, eine ca. faustgrosse weisse Muschel, deren Ende abgeschnitten ist. Ganz offensichtlich handelt es sich um eine Art Lockinstrument für die Jagd.
Gravity Models of China's Bamboo and Rattan Products Exports: Applications to Trade Potential Analysis
Authors: Huang Li , Chen Ke , Zhou Mi , Nuse Brendan 
Published: August 2021

Abstract
Using export panel data for China and 24 bamboo and rattan trading partners from 2007 to 2017, this study simulates the export trade of Chinese bamboo and rattan products using a gravity model. Our results showed that economic size has a significant positive impact on the bilateral trade of bamboo and rattan products, while absolute distance between two major economic centers and population size have a significant negative impact. Furthermore, relevant Asia-Pacific Economic Cooperation (APEC) trade arrangements have an impact on bamboo and rattan product trade flows from China. Meanwhile, trade of bamboo and rattan between China and APEC countries such as South Korea, Canada, Russia, and Thailand shows much room for growth.
Web Service Platforms, Social Networks, and Firms' Innovation Capability: Mediating Effects Model Based on Web Service Platform Synergies
Authors: Li Xiao , Li Ying , Zheng Weibo 
Published: August 2021

Abstract
With the rise of new retail concepts, commodity trading has evolved from the traditional supply-to-consumer linear single-track relationship into a brand-new Internet platform-based S2b2c social networking system.1 Despite this, over 90 percent of wood product enterprises have an extremely limited understanding and application of this new kind of web service platform-based marketing systems. To reveal the impact of web service platforms on the growth of wood product enterprises, this study proposes an analytical framework that integrates the web service platform synergy, the social network synergy, and the corporate innovation capability. Using this framework, the effects of social network and web service platform synergies on the corporate innovation capability are analyzed, with a particular focus on exploring the mediating role of web service platform synergy between the social network synergy and the corporate innovation capability. Based on the questionnaire survey data of 489 large, medium, and small-wood product enterprises in the Pearl River Delta and the Yangtze River Delta regions, the following empirical results are derived through a combination of stepwise regression and Sobel test: (1) Web service platform synergy has a significant positive impact on the corporate innovation capability; (2) Social network synergy has a significant positive impact on the corporate innovation capability; (3) Social network synergy has a significant positive impact on the establishment of web service platforms; and (4) Web service platform synergy has a partial intermediary relationship between social network synergy and innovation capability, accounting for 34.05 percent of the total effect. The results of this study indicate that like social networks, web service platforms are the strategic resources of enterprises. In the context of open innovation, it is essential for the wood product enterprises to deeply integrate with the social networks and web service platforms, which helps enhance their innovation capability.
Antibody and cytokine levels in visceral leishmaniasis patients with varied parasitemia before, during, and after treatment in patients admitted to Arba Minch General Hospital, southern Ethiopia
Authors: Tadesse Dagimawie , Abdissa Alemseged , Mekonnen Mekidim , Belay Tariku , Hailu Asrat 
Published: August 2021

BackgroundVisceral leishmaniasis is a disease caused by disseminated Leishmania donovani infection which affects almost half a million people annually. Most of the patients are reported from the Indian sub-continent, Eastern Africa and Brazil. In this study, we aimed to determine the levels of antibodies and cytokines in visceral leishmaniasis patients and to examine associations of parasitemia with the clinical states of patients. A prospective study was carried out, enrolling a total of 48 active VL patients who were evaluated before, during different time points and, three months after treatment. Serum cytokine concentrations, antibody levels, parasitemia, laboratory (hematologic and biochemical) measurements, and clinical parameters were assessed.ResultsCounts of WBC and platelets, and measurements of hemoglobin (Hb) increased during treatment (P ≤ 0.05). Elevated levels of circulating IL-10, IFN-γ, and TGF-β1 were measured before treatment. The observed increase in serum IL-10 remarkably declined within 7 days after the start of treatment. Anti-leishmanial antibody index (AI) was high in all VL patients irrespective of spleen aspirate parasite grade before treatment and at different times during treatment. However, a significant (P ≤ 0.05) decrease of AI was observed 120 days post-treatment. IL-2 serum levels were below the detection limit at all sampling points.ConclusionsThe present results suggest that IL-10, IFN-γ, and TGF-β1 can be used as markers of active visceral leishmaniasis. In addition, measuring circulating cytokines concentrations, particularly IL-10, in combination with other clinical evaluations, could be used as criteria for the cure. The observation that a high serum concentration of IFN-gamma at baseline was associated with low parasitemia deserves further investigations.
Medical malpractice related to dialysis and vascular access: An analysis of lawsuit judgements in South Korea
Authors: Kim Ji Eun , Ahn Shin Young , Cho Soo Ick , Kwon Young Joo , Shin SuHwan , Ko Gang-Jee 
Published: August 2021

Background
Hemodialysis is a life-saving renal replacement treatment for patients with chronic kidney disease, but various complications occur during hemodialysis and associated procedures. This study was conducted to analyze the specific characteristics of hemodialysis-related complications and malpractice that have led to legal disputes.

Methods
Judgments from cases litigated between 1991 and 2019 due to complications related to hemodialysis or vascular access were analyzed using the database of the Korean Supreme Court Judgment System.

Results
Of 32 dialysis-related litigation cases, 14 cases were dismissed and malpractice was recognized in 18 cases. Among all cases and those in which malpractice was recognized, the most common clinical complication was associated with central venous catheter (CVC) insertion (25.0% and 42.9%, respectively). In 22 of 32 (68.8%) cases, complications occurred before or after (not during) dialysis, and performance error was the most common clinical error leading to legal disputes (58.3%). Complications resulted in death in 59.4% of cases, and CVC-related complications were associated with the largest proportion (63.2%) of deaths.

Conclusions
Hemodialysis was implicated in various medical disputes, and CVC-related complications were the most common and serious adverse events. Clinicians’ awareness of the incidence and severity of possible complications of hemodialysis procedures should be increased.