bluuebunny/crossref_metadata_2025_split huggingface dataset:
Site of Recurrence and Survival After Surgery for Colorectal Peritoneal Metastasis
Authors: Breuer Eva , Hebeisen Monika , Schneider Marcel André , Roth Lilian , Pauli Chantal , Frischer-Ordu Katharina , Eden Janina , Pache Basile , Steffen Thomas , Hübner Martin , Villeneuve Laurent , Kepenekian Vahan , Passot Guillaume , Gertsch Philippe , Gupta Anurag , Glehen Olivier , Lehmann Kuno 
Published: January 2021

Abstract

Background
Multimodal treatment, including systemic treatment and surgery, improved the prognosis of peritoneal metastasis (PM). Despite all efforts, recurrence rates remain high, and little data are available about clinical behavior or molecular patterns of PM in comparison to hematogenous metastasis. Here, we aimed to analyze recurrence patterns after multimodal treatment for PM from colorectal cancer.

Methods
Patients with colorectal PM undergoing multimodal treatment including systemic chemotherapy and cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy (CRS/HIPEC) between 2005 and 2017 at 4 centers were analyzed retrospectively.

Results
A total of 505 patients undergoing CRS/HIPEC were analyzed. Of the patients, 82.1% received preoperative chemotherapy. Median peritoneal cancer index was 6 (interquartile range = 3-11). Median disease-free and overall survival was 12 (95% confidence interval [CI] = 11 to 14) months and 51 (95% CI = 43 to 62) months, respectively. Disease recurred in 361 (71.5%) patients, presenting as isolated peritoneal recurrence in 24.6%, isolated hematogenous recurrence in 28.3%, and mixed recurrence in 13.9% of patients. Recurrence to the peritoneum was associated with an impaired time from recurrence to death of 21 (95% CI = 18 to 31) months for isolated peritoneal and 22 (95% CI = 16 to 30) months for mixed recurrence, compared with 43 (95% CI = 31 to &gt;121) months for hematogenous recurrence (hazard ratio [HR] = 1.79, 95% CI = 1.27 to 2.53; P = .001; and HR = 2.44, 95% CI = 1.61 to 3.79; P &lt; .001). On multiple logistic regression analysis, RAS mutational status (odds ratio [OR] = 2.42, 95% CI = 1.11 to 5.47; P = .03) and positive nodal stage of the primary (OR = 3.88, 95% CI = 1.40 to 11.86; P = .01) were identified as predictive factors for peritoneal recurrence.

Conclusions
This study highlights the heterogeneity of peritoneal metastasis in patients with colorectal cancer. Recurrent peritoneal metastasis after radical treatment represents a more aggressive subset of metastatic colorectal cancer.
National Contestations of the Legal Reasoning of International Courts and Tribunals: A Gramscian Discourse Analysis Approach
Authors: Stoppioni Edoardo 
Published: January 2021

Abstract
National contestations against international courts and tribunals are a polymorph object to analyse. While they generally deal with the legitimacy of an international court or tribunal, manifesting discontent with its very existence or work, in some rare cases, national contestations focus on the legal reasoning developed in an international decision. This research will analyse this kind of national contestations in terms of ‘counterhegemonic’ reactions, using the works of Antonio Gramsci and their interpretation in neo-Gramscian scholarship. The purpose is to identify how national contestations articulate a counterhegemonic discourse to build an opposition to the reasoning of an international legal decision, and to deconstruct the linguistic tools that are adopted to facilitate this approach. Requiring a difficult exercise of welding present to future, for the counterhegemonic voice not to replicate a hegemonic discourse with simply different premisses and power intentions, the transformative project behind them is key.
Parental Psychosocial Distress in Pediatric Sickle Cell Disease and Chronic Pain
Authors: Sil Soumitri , Woodward Kerri E , Johnson Yelena L , Dampier Carlton , Cohen Lindsey L 
Published: January 2021

Abstract

Background
Pediatric sickle cell disease (SCD) management can result in considerable caregiver distress. Parents of youth with chronic SCD pain may face the additional challenge of managing children’s chronic pain and chronic illness. This study examined associations between parent psychological distress and child functioning and the moderating role of chronic pain among youth with SCD.

Methods
Youth presenting to pediatric outpatient comprehensive SCD clinics and their primary caregivers completed a battery of questionnaires. Parents reported on parenting stress, parent mental and physical health, and family functioning. Children completed measures of pain characteristics, depressive symptoms, catastrophic thinking, functional disability, and quality of life.

Results
Patients (N = 73, Mage = 14.2 years, 57% female) and their caregivers (Mage = 41.1 years, 88% mothers, 88% Black) participated. Worse parent functioning was associated with worse child pain, functioning, quality of life, and depressive symptoms. Beyond the effects of SCD, chronic SCD pain magnified the negative associations between parenting stress frequency and child quality of life, parent physical health and child quality of life, and parent depressive symptoms and child depressive symptoms.

Conclusions
Chronic pain may exacerbate the relations between parent and child functioning beyond the effects of SCD alone. The management of both SCD and chronic pain may present additional challenges for parents that limit their psychosocial functioning. Family-focused interventions to support parents and youth with chronic SCD pain are warranted to optimize health outcomes.
A RCT Testing If a Storybook Can Teach Children About Home Safety
Authors: Morrongiello Barbara A , Marquis Alexandra R , Cox Amanda 
Published: January 2021

AbstractObjectiveUnintentional injuries are the leading cause of death for children under 19 years of age. For preschoolers, many injuries occur in the home. Addressing this issue, this study assessed if a storybook about home safety could be effective to increase preschoolers’ safety knowledge and reduce their injury-risk behaviors.MethodsApplying a randomized controlled trial design, normally developing English speaking preschool children (3.5–5.5 years) in Southwestern Ontario Canada were randomly assigned to the control condition (a storybook about healthy eating, N = 30) or the intervention condition (a storybook about home hazards, N = 29). They read the assigned storybook with their mother for 4 weeks; time spent reading was tracked, and fidelity checks based on home visits were implemented.ResultsComparing postintervention knowledge, understanding score, and risk behaviors across groups revealed that children who received the intervention were able to identify more hazards, provide more comprehensive safety explanations, and demonstrate fewer risky behaviors compared with children in the control group (ηp2 = 0.13, 0.19, and 0.51, respectively), who showed no significant changes over time in safety knowledge, understanding, or risk behaviors. Compliance with reading the safety book and fidelity in how they did so were very good.ConclusionsA storybook can be an effective resource for educating young children about home safety and reducing their hazard-directed risk behaviors.
Propofol suppresses osteosarcoma cell function by regulating FOXO1/TUSC7
Authors: Huang Xinhua , Liu Jing , Xie Hong 
Published: January 2021

Abstract

Objectives
Accumulated evidence demonstrates that propofol has antitumour roles in various cancers. However, the role of propofol in osteosarcoma is still unclear. Therefore, we aim to determine the role of propofol on osteosarcoma and further explore its potential mechanism.

Methods
Cell proliferation, migration and invasion of osteosarcoma were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing and transwell assay, respectively. The interaction between FoxO1 and TUSC7 was determined using luciferase reporter assay and chromatin immunoprecipitation.

Results
Propofol treatment significantly decreased cell proliferation, migration and invasion in U2OS cells. Propofol promoted TUSC7 expression by enhancing transcriptional factor FOXO1 that leads to inactivation of AKT/GSK3β signalling resulting in the suppression of cell proliferation, migration and invasion.

Conclusions
Propofol suppresses cell proliferation, migration and invasion of osteosarcoma cells through FOXO1/TUSC7 axis by regulating AKT/GSK3β signalling.
Systemic administration of sunflower oil exerts neuroprotection in a mouse model of transient focal cerebral ischaemia
Authors: La Russa Daniele , Montesano Domenico , Pellegrino Daniela , Frisina Marialaura , Bagetta Giacinto , Fallarino Francesca , Amantea Diana 
Published: January 2021

Abstract

Objectives
Natural products are valuable sources of nutraceuticals for the prevention or treatment of ischemic stroke, a major cause of death and severe disability worldwide. Among the mechanisms implicated in cerebral ischemia-reperfusion damage, oxidative stress exerts a pivotal role in disease progression. Given the high antioxidant potential of most components of sunflower oil, we have explored its effects on ischemic brain injury produced in the mouse by transient occlusion of the middle cerebral artery (MCAo).

Key findings
Intraperitoneal (i.p.) administration of sunflower oil at doses of 3 ml/kg (48 h, 24 h and 1 h before MCAo) significantly reduced brain infarct volume and oedema assessed 24 h after the insult. This neuroprotective treatment schedule also prevented the elevation of brain lipid peroxidation produced by MCAo-reperfusion injury. By contrast, doses of 0.03 ml/kg of sunflower oil resulted ineffective on both cerebral damage and lipid peroxidation. Although sunflower oil did not affect serum levels of Diacron-reactive oxygen metabolites (d-ROMs), both 0.03 and 3 ml/kg dosing regimens resulted in the preservation of serum biological antioxidant potential (BAP) that was otherwise dramatically reduced 24 h after MCAo.

Conclusions
Sunflower oil represents a promising source of neuroprotective extracts/compounds that can be exploited for the prevention and/or treatment of cerebral ischemia.
Unusual complication after laparoscopic Nissen fundoplication
Authors: Pamart Wendy , Majerus Bernard 
Published: January 2021

Abstract
We describe a rare complication of Laparoscopic Nissen Fundoplication in the presence of a Gastric greater curvature Plication (LNFGP). An overweight 64-year-old woman was admitted for severe abdominal pain, dysphagia and fever 2 weeks after a Nissen fundoplication. She had pneumoperitoneum on scanner. At laparoscopy, we found a generalized peritonitis secondary to a perforated Nissen wrap in the presence of a gastric greater curvature plication. Gastric bypass and sleeve gastrectomy can be irrelevant in case of moderately and severely obese patients with gastroesophageal reflux disease. Four studies have investigated the risks and rewards of the LNFGP as an alternative. Only one case of leakage has been reported. We present another severe complication: the pylorus stenosis caused by a plication performed too close to the pylorus, causing gastric dilatation in the presence of the wrap, leading to wrap perforation.
Improved Spirodela polyrhiza genome and proteomic analyses reveal a conserved chromosomal structure with high abundance of chloroplastic proteins favoring energy production
Authors: Harkess Alex , McLoughlin Fionn , Bilkey Natasha , Elliott Kiona , Emenecker Ryan , Mattoon Erin , Miller Kari , Czymmek Kirk , Vierstra Richard D , Meyers Blake C , Michael Todd P 
Published: January 2021

Abstract
Duckweeds are a monophyletic group of rapidly reproducing aquatic monocots in the Lemnaceae family. Given their clonal, exponentially fast reproduction, a key question is whether genome structure is conserved across the species in the absence of meiotic recombination. Here, we studied the genome and proteome of Spirodela polyrhiza, or greater duckweed, which has the largest body plan yet the smallest genome size in the family (1C=150 Mb). Using Oxford Nanopore sequencing combined with Hi-C scaffolding, we generated a highly contiguous, chromosome-scale assembly of S. polyrhiza line Sp7498 (Sp7498_HiC). Both the Sp7498_HiC and Sp9509 genome assemblies reveal large chromosomal misorientations relative to a recent PacBio assembly of Sp7498, highlighting the need for orthogonal long-range scaffolding techniques such as Hi-C and BioNano optical mapping. Shotgun proteomics of Sp7498 verified the expression of ~2250 proteins and revealed a high abundance of proteins involved in photosynthesis and carbohydrate metabolism among other functions. In addition, a strong increase in chloroplast proteins was observed that correlated to chloroplast density. This Sp7498_HiC genome was generated cheaply and quickly with a single Oxford Nanopore MinION flow cell and one Hi-C library in a classroom setting. Combining these data with a mass spectrometry-generated proteome illustrates the utility of duckweed as a model for genomics- and proteomics-based education.
Repression of barley cathepsins, HvPap-19 and HvPap-1, differentially alters grain composition and delays germination
Authors: Gomez-Sanchez Andrea , Santamaria M Estrella , Gonzalez-Melendi Pablo , Muszynska Aleksandra , Matthess Christiane , Martinez Manuel , Diaz Isabel 
Published: January 2021

Abstract
During barley germination, cysteine proteases are essential in the mobilization of storage compounds providing peptides and amino acids to sustain embryo growth until photosynthesis is completely established. Knockdown barley plants, generated by artificial miRNA, for the cathepsins B- and F-like HvPap-19 and HvPap-1 genes, respectively, showed less cysteine protease activities and consequently lower protein degradation. The functional redundancy between proteases triggered an enzymatic compensation associated with an increase in serine protease activities in both knockdown lines, which was not sufficient to maintain germination rates and behaviour. Concomitantly, these transgenic lines showed alterations in the accumulation of protein and carbohydrates in the grain. While the total amount of protein increased in both transgenic lines, the starch content decreased in HvPap-1 knockdown lines and the sucrose concentration was reduced in silenced HvPap-19 grains. Consequently, phenotypes of HvPap-1 and HvPap-19 artificial miRNA lines showed a delay in the grain germination process. These data demonstrate the potential of exploring the properties of barley proteases for selective modification and use in brewing or in the livestock feeding industry.
Cell-Derived Microparticles in Blood Products from Blood Donors Deficient in Glucose-6-Phosphate Dehydrogenase
Authors: Noulsri Egarit , Lerdwana Surada , Palasuwan Duangdao , Palasuwan Attakorn 
Published: January 2021

Abstract

Objective
To quantitate the microparticles (MPs) in whole blood and blood products obtained from blood donors who are deficient in glucose-6-phosphate dehydrogenase (G6PD).

Methods
The current study analyzed whole blood and blood components prepared from 49 blood donors with G6PD deficiencies and 98 with G6PD-normal results. Packed red blood cells (PRBCs), platelet concentrate (PC), and plasma were prepared according to transfusion laboratory procedures. MP concentrations were determined using a flow cytometer.

Results
Blood components prepared from donors with G6PD deficiency were characterized by higher red blood cell-derived MP (RMP) concentration in PRBCs (25,526 vs 18,738 particles/µL) but lower concentrations of platelet-derived MPs (PMPs; in whole blood and PC), leukocyte-derived MPs (LMP; in whole blood and plasma) and total MP (in PC), compared with those from donors with G6PD-normal test results.

Conclusions
These results suggest that differences in G6PD status may account for variation in RMP levels during processing.
How the Word Adjacency Network (WAN) works
Authors: Brown Paul , Eisen Mark , Segarra Santiago , Ribeiro Alejandro , Egan Gabriel 
Published: January 2021

Abstract
The Word Adjacency Network (WAN) method is a newly invented method for attributing the authorship of texts based on internal evidence about the proximities of high-frequency words in those texts. The method has been the subject of mathematically rigorous explanations in scientific journals and here is offered instead a relatively non-technical account for humanist readers unfamiliar with advanced mathematical nomenclature. The description will refer to the operation of the algorithm as it is implemented in a newly completed open-source, open-access version that the authors have made available for free unlimited download on the WorldWide Web.
Nerve Reconstruction Using Processed Nerve Allograft in the U.S. Military
Authors: Dunn John C , Tadlock Joshua , Klahs Kyle J , Narimissaei Danielle , McKay Patricia , Nesti Leon J 
Published: January 2021

ABSTRACT

Background
Processed nerve allograft (PNA) is an alternative to autograft for the reconstruction of peripheral nerves. We hypothesize that peripheral nerve repair with PNA in a military population will have a low rate of meaningful recovery (M ≥ 3) because of the frequency of blasting mechanisms and large zones of injury.

Methods
A retrospective review of the military Registry of Avance Nerve Graft Evaluating Utilization and Outcomes for the Reconstruction of Peripheral Nerve Discontinuities database was conducted at the Walter Reed Peripheral Nerve Consortium. All adult active duty military patients who underwent any peripheral nerve repair with PNA for complete nerve injuries augmented with PNA visit were included. Motor strength and sensory function were reported as a consensus from the multidisciplinary Peripheral Nerve Consortium. Motor and sensory testing was conducted in accordance with the British Medical Research Council.

Results
A total of 23 service members with 25 nerve injuries (3 sensory and 22 mixed motor/sensory) underwent reconstruction with PNA. The average age was 30 years and the majority were male (96%). The most common injury was to the sciatic nerve (28%) from a complex mechanism (gunshot, blast, compression, and avulsion). The average defect was 77 mm. Twenty-four percent of patients achieved a meaningful motor recovery. Longer follow-up was correlated with improved postoperative motor function (r = 0.49 and P = .03).

Conclusions
The military population had complex injuries with large nerve gaps. Despite the low rate of meaningful recovery (27.3%), large gaps in motor and mixed motor/sensory nerves are difficult to treat, and further research is needed to determine if autograft would achieve superior results.

Type of study/Level of evidence
Therapeutic, Level III
Vancomycin enhances growth and virulence of Trichosporon spp. planktonic cells and biofilms
Authors: de Aguiar Cordeiro Rossana , da Silva Bruno Nascimento , de Aguiar Ana Luiza Ribeiro , Pereira Livia Maria Galdino , Portela Fernando Victor Monteiro , da Rocha Maria Gleiciane , Pergentino Mariana Lara Mendes , de Santos Sales Gyrliane , de Sousa José Kleybson , de Camargo Zoilo Pires , Brilhante Raimunda Sâmia Nogueira , Rocha Marcos Fábio Gadelha , Castelo-Branco Débora de Souza Collares Maia , Sidrim José Júlio Costa 
Published: January 2021

Abstract

Invasive fungal infections (IFIs) are important worldwide health problem, affecting the growing population of immunocompromised patients. Although the majority of IFIs are caused by Candida spp., other fungal species have been increasingly recognized as relevant opportunistic pathogens. Trichosporon spp. are members of skin and gut human microbiota. Since 1980’s, invasive trichosporonosis has been considered a significant cause of fungemia in patients with hematological malignancies. As prolonged antibiotic therapy is an important risk factor for IFIs, the present study investigated if vancomycin enhances growth and virulence of Trichosporon. Vancomycin was tested against T. inkin (n = 6) and T. asahii (n = 6) clinical strains. Planktonic cells were evaluated for their metabolic activity and virulence against Caenorhabditis elegans. Biofilms were evaluated for metabolic activity, biomass production, amphotericin B tolerance, induction of persister cells, and ultrastructure. Vancomycin stimulated planktonic growth of Trichosporon spp., increased tolerance to AMB, and potentiates virulence against C. elegans. Vancomycin stimulated growth (metabolic activity and biomass) of Trichosporon spp. biofilms during all stages of development. The antibiotic increased the number of persister cells inside Trichosporon biofilms. These cells showed higher tolerance to AMB than persister cells from VAN-free biofilms. Microscopic analysis showed that VAN increased production of extracellular matrix and cells in T. inkin and T. asahii biofilms. These results suggest that antibiotic exposure may have a direct impact on the pathophysiology of opportunistic trichosporonosis in patients at risk.

Lay abstract
This study showed that the vancomycin stimulated Trichosporon growth, induced morphological and physiological changes on their biofilms, and also enhanced their in vivo virulence. Although speculative, the stimulatory effect of vancomycin on fungal cells should be considered in a clinical scenario.
Stringent constraint on the radio signal from dark matter annihilation in dwarf spheroidal galaxies using the TGSS
Authors: Basu Arghyadeep , Roy Nirupam , Choudhuri Samir , Datta Kanan K , Sarkar Debajyoti 
Published: January 2021

ABSTRACT
Weakly interacting massive particles (WIMPs) are considered to be one of the favoured dark matter candidates. Searching for any detectable signal due to the annihilation and decay of WIMPs over the entire electromagnetic spectrum has become a matter of interest for the last few decades. WIMP annihilation to Standard Model particles gives rise to a possibility of detection of this signal at low radio frequencies via synchrotron radiation. Dwarf spheroidal (dSphs) galaxies are expected to contain a huge amount of dark matter which makes them promising targets to search for such large scale diffuse radio emission. In this work, we present a stacking analysis of 23 dSph galaxies observed at low frequency (147.5 MHz) as part of the TIFR-GMRT Sky Survey (TGSS). The non-detection of any signal from these stacking exercises put very tight constraints on the dark matter parameters. The best limit comes from the novel method of stacking after scaling the radio images of the individual dSph galaxy fields after scaling them by the respective half-light radius. The constraint on the thermally averaged cross-section is below the thermal relic cross-section value over a range of WIMP mass for reasonable choices of relevant astrophysical parameters. Such analysis, using future deeper observation of individual targets as well as stacking, can potentially reveal more about the WIMP dark matter properties.