bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic Hepatectomy for Mucinous Cystic Neoplasm of the liver: A Case Series of Six
Authors: Chen Yizhen , Xu Youyao , Zhang Yuhua , Han Fang , Wu Jia 
Published: May 2021

Abstract
BackgroundMucinous Cystic Neoplasm of the liver (MCN-L) is considered as a benign disease, but it has the possibility of malignant transformation. The advantages of minimally invasive surgery can alleviate the anxiety of patients with benign diseases. The purpose of this study is to determine the feasibility and safety of laparoscopic hepatectomy (LH) for patients with Mucinous Cystic Neoplasm of the liver. MethodsAll patients who were pathologically diagnosed as MCN-L were retrospectively studied. Collect the surgical data of patients undergoing laparoscopic hepatectomy, and conduct a return visit. ResultsThere were 6 cases of LH for MCN-L. The mean operation time was 176.3 ± 20.6 min, and the intraoperative blood loss was 102.5±73.7 mL. No serious complications occurred, and the mean length of hospital stay was 5.8 ± 1.5 days. During the median follow-up time of 30.5 months, no recurrence occurred. ConclusionsLH for MCN-L is feasible and safe. The treatment principle of laparoscopic surgery for MCN-L should be consistent with laparotomy.
Evaluating the national implementation of Bright IDEAS in clinical settings: applying the RE-AIM framework to characterize the clinical perspective
Authors: McNeal Demetria , Sahler O.J. , Noll Robert B. , Fairclough Diane L. , Voll Megan E. , Bhat Shubha , Morrato Elaine H. 
Published: May 2021

Abstract
Background Bright IDEAS: Problem-Solving Skills Training (Bright IDEAS), a psychosocial intervention, has shown to improve problem-solving skills and reduce emotional distress in caregivers of children recently diagnosed with cancer. Though efforts have been made to scale up cancer control interventions, there are limited data assessing the adoption of Evidence-Based Cancer Control Programs (EBCCP) into clinical practice. This study describes the barriers and facilitators to implementation experienced by practitioners trained to use Bright IDEAS in their clinical settings.Methods A total of 209 Pediatric psychosocial oncology practitioners were trained through 10 workshops. Adaptations were made to the training agenda and curriculum based on practitioner feedback following implementation in local institutions. Practitioners were interviewed to understand their training experience and gain their perspective on the effectiveness of delivering Bright IDEAS in the clinical setting. The RE-AIM framework was used to guide the evaluation process we employed to assess the effectiveness of this national effort to disseminate Bright IDEAS.Results Interviews were conducted with 47 practitioners. Practitioners in the pre-adaptation group reported the identification of a particular profile of a client as a facilitator to intervention use. Additionally, perceived rigidity of the protocol, lack of consistency in intervention use with clients, feelings of being overwhelmed by the client as rationale for lack of interest, and lack of multiple trained practitioners at institutions were identified as barriers to intervention use. Practitioners in the post-adaptation group reported similar identification of a profile for an appropriate client for the intervention, more usage of Bright IDEAS materials, implementation across multiple clinical settings, and expressed continued commitment to intervention use as well as discussion of internal training for colleagues.Conclusions This study provides insight into how psychosocial practitioners adopt, implement, and maintain Bright IDEAS in the clinical setting and identified important considerations and needs for both practitioners and larger health care systems. It also provides some cautions to those who wish to promulgate evidence-based interventions.
Effects of Institutional Framework Lapses in Solid Waste Management; Case of Ngomongo, Nairobi, Kenya
Authors: Kathambi Bessy , Ogutu Florence Akinyi 
Published: May 2021

Abstract
Institutional frameworks serve as vehicles for proper solid waste management. Lapses in institutional structures create ecological disasters that affect public health and environmental degradation. Municipal solid waste management is a critical subject worldwide, and removal and control of municipal solid waste remain a global task, specifically in developing countries. The case of Ngomongo illustrates lapses in waste institutions in Nairobi City. The research design for the study used a mixed-method where primary data was collected using transect walk, questionnaires with 385 households administered, and satellite images. Focus group discussions and key informant interviews used to ensure the validity and reliability of the data. The results show the lapses that exist in institutional frameworks in Nairobi City as supported by the objective of the study. The effects demonstrated by the doubled number of mini-landfills, open waste sites on the roadside over the decade.
Elevated coinhibitory molecule TIGIT mediates T cell exhaustion in septic patients
Authors: Sun Yini , Ding Renyu , Chang Yukun , Li Jiuming , Ma Xiaochun 
Published: May 2021

Abstract
Background: Sepsis-induced T cell exhaustion that is characterized by upregulated coinhibitory molecules and decreased cytokines release plays a crucial role in the immunosuppression during sepsis. Although PD-1 has shown a promising target to interfere with T cells dysfunction, the role of other coinhibitory receptors in sepsis remains largely elusive. Recently, it has been demonstrated that the coinhibitory molecule TIGIT more reliably identified exhausted T cells than PD-1. The aim of the study was to identify the expression of TIGIT on lymphocytes and the crucial role of TIGIT in modulating T cell function in septic patients. Methods: Twenty-five patients with sepsis and seventeen healthy controls were prospectively enrolled. Peripheral blood was obtained from septic patients within 24 hours after diagnosis of sepsis, as were healthy controls. TIGIT and other coinhibitory/costimulatory molecules expression on lymphocyte subsets was quantitated by flow cytometry. The relationship between TIGIT expression and clinical parameters was simultaneously evaluated. The function T cell from septic patients was assayed via stimulated cytokine secretion. Ex vivo functional assays were also conducted.Results: In the early stage of sepsis, patients exhibited higher levels of TIGIT on T cells relative to healthy donors, especially in the septic shock patients. Elevated frequencies of TIGIT + T cells positively correlated with the severity of organ failure and inflammatory responses in septic patients. TIGIT + T cells expressed higher levels of PD-1 and lower CD226. Further, elevated expression of TIGIT inhibited the release of cytokines including TNF, IFN-γ and IL-2 by CD4 + and CD8 + T cells. Strikingly, ex vivo blockade of TIGIT using anti-TIGIT antibody restored the frequencies of cytokine-producing T cells. Conclusions: These data illustrate TIGIT as a novel marker of exhausted T cells and suggest TIGIT may be a novel immunotherapeutic target during sepsis.
Spectrum of CECT Findings of Retained And Migrated Surgical Items In Abdomen – A Series of Seven Cases.
Authors: Kumar Ishan , Sharma Srishti , Sinha Anindita , Verma Ashish 
Published: May 2021

Abstract
Background: Various retained surgical items often present a diagnostic dilemma to the interpreting radiologist. These items include retained surgical sponge (gossypiboma), misplaced, migrated catheters, broken instruments, irrigation sets, and a variety of surgical paraphernalia. Such foreign bodies present with varying imaging findings and can often mimic other diseases radiologically.Case presentations: We present a series of seven interesting cases of retained surgical mops (case 1, 2 and 3), broken and retained surgical instrument (case 4), migrated plastic biliary stent (case 5), migrated contraceptive device (case 6) and surgically placed hemostatic agent (case 7)Conclusions: Familiarity with the variable imaging findings and communication with the clinician can facilitate timely management for these patients.
Evaluation of A Chemiluminescent Enzyme Immunoassay-Based High-Throughput SARS-CoV-2 Antigen Assay For The Diagnosis of COVID-19: The VITROS® SARS-CoV-2 Antigen Test
Authors: Matsuzaki Nanako , Orihara Yuta , Kodana Masahiro , Kitagawa Yutaro , Matsuoka Masaru , Kawamura Rieko , Takeuchi Shinichi , Imai Kazuo , Tarumoto Norihito , Maesaki Shigefumi , Takuya Maeda 
Published: May 2021

Abstract
A high-throughput, fully automated antigen detection test for SARS-CoV-2 is a viable alternative to reverse transcription polymerase chain reaction (RT-qPCR) for mass screening during outbreaks. In this study, we compared RT-qPCR for viral load and the VITROS® SARS-CoV-2 Antigen Test with reference to the results of the LUMIPULSE® SARS-CoV-2 Ag Test. Of 128 nasopharyngeal swab specimens taken from patients suspected of being infected with SARS-CoV-2, 49 were positive and 79 were negative according to RT-qPCR. Consistent dose-dependent detection with VITROS® assay was successfully achieved when using nasopharyngeal swab specimens with Ct values of ≤32.0, whereas the CLEIA-based the LUMIPULSE® assay was able to detect lower viral loads compared with the VITROS® assay. Our results show that the performance of the VITROS® assay was satisfactory for the diagnosis of contagious COVID-19 patients in the clinical setting.
Restrictive versus liberal red cell transfusion strategies in patients with acute myocardial infarction: a systematic review and meta-analysis
Authors: Liu Yuanhui , Zhang Yeshen , Huang Yuming , Xie Nianjin , Zeng Lihuan , Lian Xingji , Lin Jijin , Dai Yining , Chen Jiyan , He Pengcheng , Tan Ning 
Published: May 2021

Abstract
BackgroundAnemia is frequent in patients with acute myocardial infarction(AMI), and the optimal red blood cell transfusion strategy for patients with AMI and anemia is still controversial. We aimed to compare the efficacy of restrictive and liberal red cell transfusion strategies in patients with AMI and anemia.MethodsWe systematically searched PubMed, EMBASE, Web of Science, Cochrane Library, and Clinicaltrials.gov, from their inception until March 2021. Inclusion criteria were clinical trials that compared the efficacy of restrictive and liberal transfusion strategies in patients with AMI and anemia on all-cause mortality and major adverse cardiovascular events. The primary outcome was all-cause mortality, including overall mortality, in-hospital, or follow-up mortality. Risk ratios (RR) with 95% confidence intervals (CI) were presented and pooled by random-effects models. ResultsThe search yielded a total of 6630 participants in six studies. Among the included patients, the average age ranged from 69.0 to 79.5 years and 2950 (44.5%) were men. A total of 2008 patients received restrictive red blood transfusion while 4622 patients were given liberal red blood transfusion. No difference was found in overall mortality and follow-up mortality between restrictive and liberal blood transfusion groups (RR=1.07, 95% CI=0.82–1.40, P=0.62; RR=0.89, 95% CI=0.56–1.42, P=0.62). However, restrictive transfusion was associated with a higher risk of in-hospital mortality compared with liberal transfusion (RR=1.22, 95% CI=1.00–1.50, P=0.05). No secondary outcomes, including follow-up reinfarction, stroke, and acute heart failure, differed significantly between the two groups. In addition, subgroup analysis showed no differences in overall mortality between the two groups based on sample size and design.ConclusionRestrictive and liberal red blood transfusion have a similar effect on overall mortality and follow-up mortality in patients with AMI and anemia. However, restrictive transfusion was associated with a higher risk of in-hospital mortality compared with liberal transfusion. The findings suggest that transfusion strategy should be evaluated in the more future researches.
EGR1 Modulated LncRNA HNF1A-AS1 Drives Glioblastoma Progression Via miR-22-3p/ENO1 Axis
Authors: Ma Chunchun , Wang Hongliang , Zong Gang , He Jie , Wang Yuyang , Yang Fan , Yang Zhihao , Bian Erbao , Zhao Bing 
Published: May 2021

Abstract
Background: Accumulating evidences revealed that long noncoding RNAs (lncRNAs) have been participated in cancer malignant progression, including glioblastoma multiforme (GBM). Despite much studies have found the precise biological role in the regulatory mechanisms of GBM，however the molecular mechanisms，particularly upstream mechanisms still need further elucidated. Methods: RT-QPCR, cell transfection, western blotting and bioinformatic analysis were executed to detect the expression of EGR1, HNF1A-AS1, miR-22-3p and ENO1 in GBM. Cell proliferation assay, colony formation assay, wound healing, migration and invasion assays were performed to detect the malignant characters of GBM cell. The molecular regulation mechanism was confirmed by luciferase reporter assay, ChIP and RIP. Finally, orthotopic mouse models were established to examine the effect of HNF1A-AS1 in vivo.Results: In the current study, we analyzed clinical samples to show that the long non-coding antisense transcript of HNF1A, HNF1A-AS1, is upregulated and associated with poor prognosis in GBM. Functional studies revealed that knockdown of HNF1A-AS1 markedly inhibits cell proliferation, migration and invasion both in vitro and in vivo, whereas overexpression of HNF1A-AS1 exerts opposite effect. Mechanistically, the transcription factor EGR1 forced the transcription of HNF1A-AS1 by directly binding the promoter region of HNF1A-AS1. Furthermore, combined bioinformatics analysis with our mechanistic work, using luciferase reporter assays and RIP, we first demonstrated that HNF1A-AS1 functions as a competing endogenous RNA (ceRNA) with miR-22-3p to regulate ENO1 expression in GBM cells. HNF1A-AS1 directly binds to miR-22-3p and significantly inhibits miR-22-3p expression, while ENO1 expression was increased. miR-22-3p inhibitor offsets the HNF1A-AS1 silencing induced suppression in proliferation, migration and invasion of GBM cells, as well as promotion effect on ENO1 expression. ENO1 was verified as a direct target of miR-22-3p and its expression levels was negatively with the prognosis in GBM patients. Conclusion: Taken together, our study illuminated the definite mechanism of HNF1A-AS1 in promoting GBM malignancy, and provided a novel therapeutic target for further clinical application.
Germplasm Characterization and Flowering, Fruiting Nature of Burmese Grape (Baccaurea Sapida Muell. Arg.) –a Potential Fruit Crop for Nutrition in Rural South East Asia
Authors: BHOWMICK NILESH , Pradhan Saurabh 
Published: May 2021

Abstract
Burmese grape (Baccaurea sapida Muell. Arg.) belongs to the family Euphorbiaceae, is a minor underexploited fruit crop grown wild as well as under cultivation in Nepal, India, Myanmar, Bangladesh, China, Thailand, for fresh consumption. It is a dioecious plant and reported to have an excellent nutritive value, but mainly grown in homestead condition mostly in neglected ways without fullest utilization of its potentiality. The present study was aimed to understand and first time characterize the germplasm nature, and details of flowering and fruiting system of Burmese grape. The experiment showed that, tree growth habit and crown shape of all the accessions surveyed were semi erect and irregular, respectively. The flower bud differentiation in male and female accessions started during the mid February and anthesis occurred from mid March for both male and female accessions. The colour of the male flowers varied from yellow to green yellow shades while the colour of the female flowers varied from yellow to yellow green and green yellow shades. Significant variation of leaf area, number of flowers per panicle, length and diameter of inflorescence, fruit length, breadth, weight etc. were observed. The variation present on fruit length (2.81 cm to 3.80 cm), fruit weight (12.25g to 22.95g), pulp percentage (49.82% to 65.33%), total soluble solids (9.00º to 13.10ºBrix), tritable acidity (0.73mg to 1.28mg/100g fruit pulp) and ascorbic acid content ranged from (23.18mg to 57.80mg/100g fruit pulp) suggest suitable selection method for identification of promising Burmese grape genotypes.
Clival Metastases in Cancer Patients
Authors: Sturgis Rebecca , Mack Alleda , Kim Seongho , Maier Jordan , Heath Elisabeth 
Published: May 2021

Abstract
Purpose

This study aimed to describe clinical features, radiotherapy (RT), and symptom outcomes in cancer patients with cranial nerve palsies associated with clival metastases.
Methods

A retrospective record review for the period in between 2000 and 2020 was conducted for patients with primary metastatic cancers, who developed distal clival metastases, or were treated with RT at the Karmanos Cancer Institute (Detroit, Michigan). The patients’ demographics and clinical characteristics, including their symptoms and improvement of symptoms after RT are described.
Results

Forty-four patients were identified who met inclusion criteria. The most common primary cancers were breast cancer, prostate cancer, and multiple myeloma. Magnetic resonance images and computed tomography scans were used for the diagnosis of clival metastasis, as well as for the evaluation after RT. Thirty-two patients (73%) with clival metastases also had cervical spine metastases. Prevailing neurologic symptoms were headache, diplopia, lateral gaze paralysis, blurry vision, chin numbness, and tongue deviation. Fifteen of 23 RT-treated patients (65%) received clivus only RT, and 8 patients (35%) were given whole brain RT. Post-RT symptom improvement was observed in patients with diplopia (5/6; 83%), headache (8/10; 80%), chin numbness (2/4; 50%), blurry vision (2/5; 40%), lateral gaze deficit (2/6; 33%), and tongue deviation (1/4; 25%).
Conclusions

These results suggest that early detection and rigorous cranial nerve examination, in addition to RT treatment, should be considered in patients with breast cancer, prostate cancer, and multiple myeloma, who developed bone metastasis, especially cervical spine metastasis.
Meta-Analysis of The Association Between Gene Mutation and Tumor Spread Through Air Space in Lung Adenocarcinoma
Authors: Wang Guishi , Li Bingfu , Dingqian Zhang , He Zhenyv , Wang Tiantian , Dong Yanjun 
Published: May 2021

Abstract
Background A series of studies have shown that spread through air spaces (STAS) has a significant effect on surgical outcomes in patients with lung adenocarcinoma. Objectives: The aims of this study are to evaluate the association between gene mutation and STAS in lung adenocarcinoma for preoperative prediction of STAS in lung adenocarcinoma, eventually, which could help us choose appropriate surgical type. Methods The retrieval period was from 2015 to December 2020. The Newcastle-Ottawa quality assessment scale (NOS) was used for assessing the quality of each study. High-quality study was defined as those with ≥6 stars at the NOS. Statistical results were analyzed using the Stata16 statistical package. Results A total of 12 studies were included, including 4790 patients. The results of meta-analysis showed that: In patients with lung adenocarcinoma, the expression of EGFR, KRAS, BRAF, ALK and ROS1 were correlated with the occurrence of STAS, with RR values (95%CI) of 0.75 (0.63~0.90), 1.173 (1.00~1.37), 2.116 (1.17~3.84), 1.71 (1.53~1.90), 1.565 (1.35~1.82), respectively. Conclusion Mutations in KRAS, BRAF, ALK, and ROS1 increase the incidence of STAS in patients with lung adenocarcinoma. Mutations in EGFR decrease the incidence of STAS in patients with lung adenocarcinoma.
LPI-DeepGBDT: A Multiple-Layer Deep Framework based on Gradient Boosting Decision Trees for lncRNA-Protein Interaction Identification
Authors: ZhouZhou Liqian , Wang Zhao , Tian Xiongfei , Peng Lihong 
Published: May 2021

Abstract
Background: Long noncoding RNAs (lncRNAs) play important roles in various biological and pathological processes. Discovery of lncRNA-protein interactions (LPIs) contributes to understand the biological functions and mechanisms of lncRNAs. Although wet experiments find a few interactions between lncRNAs and proteins, experimental techniques are costly and time-consuming. Therefore, computational methods are increasingly exploited to uncover the possible associations. However, existing computational methods have several limitations. First, majority of them were measured based on one simple dataset, which may result in the prediction bias. Second, few of them are applied to identify relevant data for new lncRNAs (or proteins). Finally, they failed to utilize diverse biological information of lncRNAs and proteins. Results: Under the feed-forward deep architecture based on Gradient Boosting Decision Trees (LPI-deepGBDT), this work focuses on classify unobserved LPIs. First, three human LPI datasets and two plant LPI datasets are arranged. Second, the biological features of lncRNAs and proteins are extracted by Pyfeat and BioProt, respectively. Thirdly, the features are dimensionally reduced and concatenated as a vector to represent an lncRNA-protein pair. Finally, a deep architecture composed of the forward mappings and inverse mappings is developed to predict underlying linkages between lncRNAs and proteins. LPI-deepGBDT is compared with four classical LPI prediction models (LPI-BLS, LPI-CatBoost, PLIPCOM, and LPI-SKF) under three cross validations on lncRNAs, proteins, lncRNA-protein pairs, respectively. It obtains the best average AUC and AUPR values on the five datasets under the three cross validations, significantly outperforming other four LPI identification methods. That is, AUCs computed by LPI-deepGBDT are 0.8321, 0.6815, and 0.9073, respectively and AUPRs are 0.8095, 0.6771, and 0.8849, respectively. The results demonstrate the powerful classification ability of LPI-deepGBDT. Case study analyses show that there may be interactions between GAS5 and Q15717, RAB30-AS1 and O00425, and LINC-01572 and P35637. Conclusions: Integrating ensemble learning and hierarchical distributed representations and building a multiple-layered deep architecture, this work improves LPI prediction performance as well as effectively probes interaction data for new lncRNAs/proteins.