bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex Differences in Trends and In‐Hospital Outcomes Among Patients With Critical Limb Ischemia: A Nationwide Analysis
Authors: Elbadawi Ayman , Barssoum Kirolos , Megaly Michael , Rai Devesh , Elsherbeeny Ahmed , Mansoor Hend , Shishehbor Mehdi H. , Abdel‐Latif Ahmed , Gulati Martha , Elgendy Islam Y. 
Published: September 2021

Background
Critical limb ischemia (CLI) represents the most severe form of peripheral artery disease and is associated with significant mortality and morbidity. Contemporary data comparing the sex differences in trends, revascularization strategies, and in‐hospital outcomes among patients with CLI are scarce.

Methods and Results

Using the National Inpatient Sample database years 2002 to 2015, we identified hospitalizations for CLI. Temporal trends for hospitalizations for CLI were evaluated. The differences in demographics, revascularization, and in‐hospital outcomes between both sexes were compared. Among 2 400 778 CLI hospitalizations, 43.6% were women. Women were older and had a higher prevalence of obesity, hypertension, heart failure, and prior stroke. Women were also less likely to receive any revascularization (34.7% versus 35.4%,
P
<0.001), but the trends of revascularization have been increasing among both sexes. Revascularization was associated with lower in‐hospital mortality among women (adjusted odds ratio [OR], 0.76; 95% CI, 0.71–0.81) and men (adjusted OR, 0.69; 95% CI, 0.65–0.73). On multivariable analysis adjusting for patient‐ and hospital‐related characteristics as well as revascularization, women had a higher incidence of in‐hospital mortality, postoperative hemorrhage, need for blood transfusion, postoperative infection, ischemic stroke, and discharge to facilities compared with men.

Conclusions
In this nationwide contemporary analysis of CLI hospitalizations, women were older and less likely to undergo revascularization. Women had a higher incidence of in‐hospital mortality and bleeding complications compared with men. Sex‐specific studies and interventions are needed to minimize these gaps among this high‐risk population.
The influence of ENSO on Arctic sea ice in large ensembles and observations
Authors: Clancy Robin , Bitz Cecilia , Blanchard-Wrigglesworth Ed 
Published: September 2021

AbstractThe El Niño-Southern Oscillation (ENSO) and its teleconnections form the leading mode of interannual variability in the global climate system, yet the small sample size of ENSO events during which we have reliable Arctic observations makes constraining its influence on Arctic sea ice challenging. We compare the influence of ENSO on Arctic sea ice in six models from the Multi-Model Large Ensemble Archive to that in observations. Each model simulates reduced Arctic sea ice area and volume in the seasons following an El Niño compared to a La Niña. The spatial patterns of sea ice concentration and thickness responses to ENSO are spatially heterogeneous, with regions of increased and decreased sea ice. The small sample size of ENSO events in observations is shown to preclude a statistically significant sea ice response from being identified. While models agree with one another on many aspects of the sea ice response to ENSO, some features are model-dependent. For example, the CESM1-LE alone displays a delayed melting response in summer, driven by reduced surface albedo and increased shortwave absorption. A positive Arctic Oscillation and a deepened Aleutian Low are common responses to ENSO across models and observations. These patterns of atmospheric variability are quantitatively shown to be key in linking ENSO to Arctic sea ice in most models, acting primarily through sea ice dynamics to generate anomalous sea ice thickness and concentration patterns.
Engaging with stakeholders to produce actionable science: a framework and guidance
Authors: Bamzai-Dodson Aparna , Cravens Amanda E. , Wade Alisa , McPherson Renee A. 
Published: September 2021

AbstractNatural and cultural resource managers are increasingly working with the scientific community to create information on how best to adapt to the current and projected impacts of climate change. Engaging with these managers is a strategy that researchers can use to ensure that scientific outputs and findings are actionable (or useful and usable). In this article, the authors adapt Davidson’s wheel of participation to characterize and describe common stakeholder engagement strategies across the spectrum of Inform, Consult, Participate, and Empower. This adapted framework provides researchers with a standardized vocabulary for describing their engagement approach, guidance on how to select an approach, methods for implementing engagement, and potential barriers to overcome. While there is often no one “best” approach to engaging with stakeholders, researchers can use the objectives of their project and the decision context in which their stakeholders operate to guide their selection. Researchers can also revisit this framework over time as their project objectives shift and their stakeholder relationships evolve.
Genetic characterization of Carbapenem-Resistant Escherichia coli from China, 2015–2017
Authors: Li Fengtian , Ye Kun , Li Xin , Ye Liyan , Guo Ling , Wang Lifeng , Yang Jiyong 
Published: September 2021

Abstract
Background
The molecular characteristics of carbapenem-resistant Escherichia coli (CREco) remain unclear.

Methods
We conducted a multi-center bacterial resistance monitoring project from 2015 to 2017.The minimum inhibitory concentrations ofCREco were determined bybroth microdilution method. The genome sequencing of CREcoisolates was performed, and single-nucleotide polymorphism (SNP) was analyzed.

Results
A total of 144CREcoisolatescollected from 10 cities in China were involved in this study. ST167 (n = 43) is the most popular type, followed by ST410(n = 14), ST131(n = 9). There were 102 (70.83%) CREco isolates that produced various NDMs, including NDM-1 (n = 16), NDM-4(n = 1), NDM-5(n = 79), NDM-6(n = 2) and NDM-9(n = 4). In addition, 15 isolates produced KPC-2, three isolates wereIMP-4 positive, and three isolates produced OXA-48. Genetic relatedness and phylogenetic analysis showed that isolates with the same ST had a high degree of homology. Some STs (including ST167, ST410, ST131, ST46, ST405 and ST617) exhibited a trend of outbreak.

Conclusions
The majority of CREco belonged to ST167, followed by ST410 and ST131, and most of them carried various NDM-coding genes. The spread of high-risk clones of CREco has occurred in different regions of China.
How does air pollution affect urban settlement of the floating population in China? New evidence from a push-pull migration analysis
Authors: Zhao Zhihao , Lao Xin , Gu Hengyu , Yu Hanchen , Lei Ping 
Published: September 2021

Abstract
Background
Severe air pollution in China threatens human health, and its negative impact decreases the urban settlement intentions of migrants in destination cities. We establish a comprehensive framework based on the push-pull migration model to investigate this phenomenon.

Methods
We employ a logistic model to analyze air pollution’s impact on the settlement intentions of the floating population based on the CMDS 2017 in China, combining the city-level socioeconomic variables with the individual-level variables.

Results
Our results show that the annual average concentration of PM2.5 increases by 1 unit and that the probability of migrants’ settlement intentions will decrease by 8.7%. Using a heterogeneity analysis, we find that the following migrant groups are more sensitive to air pollution: males, people over 30 years old, less educated people, and migrants with nonagricultural hukou. With every 1 unit increase in PM2.5, each group’s settlement intentions decrease by 13.2, 16.7, 16.9, and 12.6%, respectively.

Conclusions
Our results are consistent with existing studies. This study discovers that both external environment and internal factors influence migrants’ settlement intentions. Specifically, the differences in population sizes, economic development levels, public services, infrastructure conditions, and environmental regulations between cities play a significant role in migration decisions. We also confirm heterogeneous sensitivities to air pollution of different migrant subgroups in terms of individual characteristics, family factors, migration features, social and economic attributes.
Laparoscopic cholecystectomy: review of 1430 cases in Cure International Hospital, Kabul, Afghanistan
Authors: Farda Wais , Tani Mohammad Kamal , Manning Richard G. , Fahmi Mohammad Samim , Barai Nasratullah 
Published: September 2021

Abstract
Background
Laparoscopic cholecystectomy (LC) is the gold standard for the treatment of cholelithiasis in most countries of the world. The objective of this study was to evaluate the outcomes of LC in the surgery department of Cure International Hospital, Kabul, Afghanistan.

Methods
A retrospective study was conducted on 1430 LC cases performed by the general surgery department of Cure International Hospital. Data was collected from patient files and the operation theatre registry for whom LC was performed during January 2008 through December, 2019.

Results
Mean age was 45.77 ± 13.45 years (14–90 years), with male/female ratio of 1:4.7. One third (33%) had comorbidities. Most of patients (~ 97%) were classified as ASA grade I and II. Of all patients, 26.8% of males and 13.2% of females had gallbladder inflammation (OR = 2.203, 95% CI 1.56–2.61, P = 0.000). Overall mean duration of anesthesia was 75 ± 25.6 min. The conversion rate to OC was 4.6% (N = 66), most commonly dense adhesions at Callot’s triangle (3.8%). The intraoperative complication rate was 17.5% (N = 249), where bile/stone spillage was the most common indication (N = 235, 16.4%). Immediate postoperative complication rate was 2.4% (N = 35). Average length of stay (ALOS) after LC was 2.23 ± 1.43 days (1–19 days).

Conclusion
This study shows that elective LC can be performed safely in Afghanistan with comparable outcomes in terms of complications, conversion rates, and ALOS to other countries of the region and the world. Proper case selection and careful preoperative evaluation and management can decrease further conversion, intra- and postoperative complications.
Oral health seeking behaviors of adults in Nebbi District, Uganda: a community-based survey
Authors: Ocwia Juliet , Olum Ronald , Atim Pamela , Laker Florence , Okot Jerom , Sereke Senai Goitom , Baluku Joseph Baruch , Kiguli Sarah , Bongomin Felix 
Published: September 2021

Abstract
Background
Dental health is often neglected by the majority of the population and has contributed to the global burden of oral diseases. We assessed awareness, utilization and barriers to seeking oral health care among adults in Nebbi District, Uganda.

Methods
A community-based, cross sectional study was conducted in the central division, Nebbi District in Uganda among adults between the age of 18 years or older. An interviewer-administered, semi-structured questionnaire was used for data collection on socio-demographic characteristics, oral health awareness, oral health utilization, associated factors and barriers.

Results
A total of 400 adults with a median age of 32 years (interquartile range 24–43) years were enrolled. More than half (57.5%, n = 230) of the participants were female. Participants identified smoking (42.8%, n = 171) and consumption of sugary foodstuffs (29.0%, n = 116) as risk factors for oral disease. Not brushing was also identified by 260 participants (65.0%) as the cause of tooth decay and 95.8% (n = 383) believed brushing one’s teeth could prevent tooth decay. Of the 51.5% (n = 206) who had experienced a toothache or discomfort 12 months prior to the study but only about half (52%, n = 106) had sought healthcare from a dental clinic or facility. About 89.5% (n = 94) of the participants were able to see a dentist during their last visits. Dental carries (76.6%, n = 70) and gum bleeding (14.9%, n = 14) were the most frequent reasons for visiting a dental clinic, and 73.7% (n = 70) had their tooth extracted. Barriers to seeking oral healthcare were cost of treatment (47.5%, n = 190), and long waiting time (18.5%, n = 74). The odds of seeking oral healthcare was 2.8-fold higher in participants who were being married (Odds ratio (OR): 2.8, 95% CI 1.3–6.3, p = 0.011) and 3.5-fold higher among housewives (COR: 3.5, 95% CI 1.1–11.4, p = 0.040).

Conclusion
About half of the participants had sought healthcare following a dental condition. Cost of treatment seems to be an important factor affecting utilization of oral health services. Optimization of costs, and creating awareness regarding benefits of utilizing preventive dental services are recommended.
Parental perception of neonatal transfers from level 3 to level 2 neonatal intensive care units in Calgary, Alberta: qualitative findings
Authors: Dosani Aliyah , Murthy Prashanth , Kassam Shafana , Rai Baldeep , Lodha Abhay K. 
Published: September 2021

Abstract
Background
Retro-transfers from level 3 to 2 NICUs in Alberta’s regionalization of neonatal care system are essential to ensure the proper utilization of level 3 NICUs for complex neonatal cases. Parents often experience distress that relates to the transfer of their neonates to another hospital. Limited information is available regarding parental perceptions of distress during transfers for neonates requiring care between NICUs in the current Canadian context. The objective of this study was to investigate: 1) what caused parents distress and could be changed about the transfer process and 2) the supports that were available to help ease parental distress during the transfer process.

Methods
Parents of singleton infants retro-transferred from level 3 to 2 NICUs in Calgary, Alberta between January 1, 2016, and December 31, 2017, were invited to participate in the study. Questionnaires were self-administered by one parent per family. A thematic deductive approach was employed by the researchers to analyze the qualitative data.

Results
Our response rate was 39.1% (n = 140). We found three themes for causes of parental distress and supports available to ease parental distress during the transfer, including communication between staff members and parents, details about the transfer process, and the care received throughout and shortly after the transfer process.

Conclusion
Parents should receive at least 24 h of notice, regular transfer updates, employ anticipatory preparation strategies, and foster more open communication between parents and health care professionals to help ensure parental satisfaction.
Cisplatin combined with capecitabine-induced chemotherapy for local nasopharyngeal carcinoma can improve the quality of life and reduce toxic and side effects
Authors: Gao Ying , Liu Zhe , Liu Yiting 
Published: September 2021

Abstract
Background
This study was designed to probe into the effect of cisplatin combined with capecitabine on nasopharyngeal carcinoma (NPC).

Methods
A total of 136 NPC patients treated for the first time in our hospital from January 2016 to March 2017 were collected and divided into two groups: A and B. Among them, 66 in group A were treated with cisplatin intravenous drip, while 70 in group B were treated with capecitabine on the basis of group A. The efficacy, toxic and side effects, and quality of life of the two groups were observed.

Results
The short-term efficacy of group B was better than that of group A (p<0.05). The toxic and side effects of group B were lower than that of group A (p<0.05). The quality of life in group B was higher than that in group A (p<0.05).

Conclusions
Cisplatin combined with capecitabine-induced chemotherapy for local NPC can improve the quality of life and reduce the toxic and side effects.
Up-regulation of GSTT1 in serous ovarian cancer associated with resistance to TAXOL / carboplatin
Authors: Zhang Jing , Xie Suhong , Zhou Lei , Tang Xiaoyu , Guan Xiaolin , Deng Minjie , Zheng Hui , Wang Yanchun , Lu Renquan , Guo Lin 
Published: September 2021

AbstractSerous ovarian cancer (SOC) is the most common women cancer and the leading cause of cancer-related mortality among the gynaecological malignancies. Although effective chemotherapeutics combined with surgery are developed for the treatment, the five-year survival rate is unsatisfactory due to chemoresistance. To overcome this shortcoming of chemotherapy, we established taxol and carboplatin resistant SOC cell lines for the understandings of the molecular and cellular mechanisms of chemoresistance. Here, we found that these chemoresistant cell lines showed less viability and proliferation, due to more cells arrested at G0/G1 phase. Glutathione-S-transferases-theta1 (GSTT1) was significantly upregulated in these chemoresistant cells, along with other chemoresistant genes. Meanwhile, GSTT1 expression was also significantly upregulated in the SOC patient tissues after taxol treatment, indicating this upregulation was physiologically relevant to chemotherapy. Further, suppression of GSTT1 expression by shRNA in SOC cell lines led to more sensitivity to drug treatment, through increasing divided cells and promoting cell death. Moreover, the expression of DNA topoisomerase 1 (Topo I) was in synergy with that of GSTT1 in the chemoresistant cells, and GSTT1 can bind to Topo I in vitro, which suggested GSTT1 could function through DNA repair mechanism during chemoresistance. In summary, our data imply that GSTT1 may be a potential biomarker or indicator of drug resistance in serous ovarian cancer.
Changes in patients’ outlook, behaviors, and attitudes toward COVID-19 after hospitalization and their experiences of discrimination and harassment
Authors: Goda Ken , Kenzaka Tsuneaki , Yahata Shinsuke , Kumabe Ayako , Katsurada Masahiro , Nishisaki Hogara 
Published: September 2021

Abstract
Objective
This study aims to examine changes in patients’ perspectives and outlooks regarding the disease and their health after hospitalization for COVID-19 and investigate their discrimination and harassment experiences. This prospective observational study surveyed discharged patients who had been admitted to Hyogo Prefectural Tamba Medical Center in Japan for COVID-19. Patient characteristics, changes in outlook and behaviors after discharge, and incidents of discrimination and harassment were examined. The study was conducted in two waves: March–June 2020 and July–September 2020.

Results
Responses were obtained from 27 patients aged 50 ± 17 years, including 16 men (59.3%). We found most patients feared infection before hospitalization (88.5%) and had taken some preventive measures (96.3%), however after discharge, all (100%) practiced social distancing and infection prevention. Twenty patients (80%) considered changing their lifestyles, and 19 (79.2%) decided to use sick leave when they felt ill; these trends were more prominent during the second wave. Six patients (23.1%) reported experiencing discrimination or harassment after discharge. While most patients with COVID-19 had a strong fear of infection before hospitalization, their views about health and health behaviors changed after hospitalization.
Whole genome sequencing of Enterobacter mori, an emerging pathogen of kiwifruit and the potential genetic adaptation to pathogenic lifestyle
Authors: Zhang Mingyang , Zhang Yilin , Han Xue , Wang Juan , Yang Yu , Ren Biao , Xia Mian , Li Gang , Fang Rongxiang , He Hang , Jia Yantao 
Published: September 2021

AbstractMembers of the Enterobacter genus are gram-negative bacteria, which are used as plant growth-promoting bacteria, and increasingly recovered from economic plants as emerging pathogens. A new Enterobacter mori strain, designated CX01, was isolated as an emerging bacterial pathogen of a recent outbreak of kiwifruit canker-like disease in China. The main symptoms associated with this syndrome are bleeding cankers on the trunk and branch, and brown leaf spots. The genome sequence of E. mori CX01 was determined as a single chromosome of 4,966,908 bp with 4640 predicted open reading frames (ORFs). To better understand the features of the genus and its potential pathogenic mechanisms, five available Enterobacter genomes were compared and a pan-genome of 4870 COGs with 3158 core COGs were revealed. An important feature of the E. mori CX01 genome is that it lacks a type III secretion system often found in pathogenic bacteria, instead it is equipped with type I, II, and VI secretory systems. Besides, the genes encoding putative virulence effectors, two-component systems, nutrient acquisition systems, proteins involved in phytohormone synthesis, which may contribute to the virulence and adaption to the host plant niches are included. The genome sequence of E. mori CX01 has high similarity with that of E. mori LMG 25,706, though the rearrangements occur throughout two genomes. Further pathogenicity assay showed that both strains can either invade kiwifruit or mulberry, indicating they may have similar host range. Comparison with a closely related isolate enabled us to understand its pathogenesis and ecology.
A study on catalytic and non-catalytic sites of H5N1 and H1N1 neuraminidase as the target for chalcone inhibitors
Authors: Hariyono Pandu , Kotta Jasvidianto Chriza , Adhipandito Christophorus Fideluno , Aprilianto Eko , Candaya Evan Julian , Wahab Habibah A. , Hariono Maywan 
Published: September 2021

AbstractThe H1N1 pandemic in 2009 and the H5N1 outbreak in 2005 have shocked the world as millions of people were infected and hundreds of thousands died due to the infections by the influenza virus. Oseltamivir, the most common drug to block the viral life cycle by inhibiting neuraminidase (NA) enzyme, has been less effective in some resistant cases due to the virus mutation. Presently, the binding of 10 chalcone derivatives towards H5N1 and H1N1 NAs in the non-catalytic and catalytic sites was studied using molecular docking. The in silico study was also conducted for its drug-like likeness such as Lipinski Rule, mutagenicity, toxicity and pharmacokinetic profiles. The result demonstrates that two chalcones (1c and 2b) have the potential for future NA inhibitor development. Compound 1c inhibits H5N1 NA and H1N1 NA with IC50 of 27.63 µM and 28.11 µM, respectively, whereas compound 2b inhibits NAs with IC50 of 87.54 µM and 73.17 µM for H5N1 and H1N1, respectively. The in silico drug-like likeness prediction reveals that 1c is 62% better than 2b (58%) in meeting the criteria. The results suggested that 1c and 2b have potencies to be developed as non-competitive inhibitors of neuraminidase for the future development of anti-influenza drugs.