bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aptamer-assisted tumor localization of bacteria for enhanced biotherapy
Authors: Geng Zhongmin , Cao Zhenping , Liu Rui , Liu Ke , Liu Jinyao , Tan Weihong 
Published: November 2021

AbstractDespite bacterial-mediated biotherapies have been widely explored for treating different types of cancer, their implementation has been restricted by low treatment efficacy, due largely to the absence of tumor-specific accumulation following administration. Here, the conjugation of aptamers to bacterial surface is described by a simple and cytocompatible amidation procedure, which can significantly promote the localization of bacteria in tumor site after systemic administration. The surface density of aptamers can be easily adjusted by varying feed ratio and the conjugation is able to increase the stability of anchored aptamers. Optimal bacteria conjugated with an average of 2.8 × 105aptamers per cell present the highest specificity to tumor cells in vitro, separately generating near 2- and 4-times higher accumulation in tumor tissue at 12 and 60 hours compared to unmodified bacteria. In both 4T1 and H22 tumor-bearing mouse models, aptamer-conjugated attenuated Salmonella show enhanced antitumor efficacy, along with highly activated immune responses inside the tumor. This work demonstrates how bacterial behaviors can be tuned by surface conjugation and supports the potential of aptamer-conjugated bacteria for both targeted intratumoral localization and enhanced tumor biotherapy.
Towards prediction of ordered phases in rechargeable battery chemistry via group–subgroup transformation
Authors: Ran Yunbing , Zou Zheyi , Liu Bo , Wang Da , Pu Bowei , Mi Penghui , Shi Wei , Li Yajie , He Bing , Lu Ziheng , Lu Xia , Li Baihai , Shi Siqi 
Published: November 2021

AbstractThe electrochemical thermodynamic and kinetic characteristics of rechargeable batteries are critically influenced by the ordering of mobile ions in electrodes or solid electrolytes. However, because of the experimental difficulty of capturing the lighter migration ion coupled with the theoretical limitation of searching for ordered phases in a constrained cell, predicting stable ordered phases involving cell transformations or at extremely dilute concentrations remains challenging. Here, a group-subgroup transformation method based on lattice transformation and Wyckoff-position splitting is employed to predict the ordered ground states. We reproduce the previously reported Li0.75CoO2, Li0.8333CoO2, and Li0.8571CoO2 phases and report a new Li0.875CoO2 ground state. Taking the advantage of Wyckoff-position splitting in reducing the number of configurations, we identify the stablest Li0.0625C6 dilute phase in Li-ion intercalated graphite. We also resolve the Li/La/vacancy ordering in Li3xLa2/3−xTiO3 (0 < x < 0.167), which explains the observed Li-ion diffusion anisotropy. These findings provide important insight towards understanding the rechargeable battery chemistry.
Unveiling the orbital texture of 1T-TiTe2 using intrinsic linear dichroism in multidimensional photoemission spectroscopy
Authors: Beaulieu Samuel , Schüler Michael , Schusser Jakub , Dong Shuo , Pincelli Tommaso , Maklar Julian , Neef Alexander , Reinert Friedrich , Wolf Martin , Rettig Laurenz , Minár Ján , Ernstorfer Ralph 
Published: November 2021

AbstractThe momentum-dependent orbital character in crystalline solids, referred to as orbital texture, is of capital importance in the emergence of symmetry-broken collective phases, such as charge density waves as well as superconducting and topological states of matter. By performing extreme ultraviolet multidimensional angle-resolved photoemission spectroscopy for two different crystal orientations linked to each other by mirror symmetry, we isolate and identify the role of orbital texture in photoemission from the transition metal dichalcogenide 1T-TiTe2. By comparing our experimental results with theoretical calculations based on both a quantitative one-step model of photoemission and an intuitive tight-binding model, we unambiguously demonstrate the link between the momentum-dependent orbital orientation and the emergence of strong intrinsic linear dichroism in the photoelectron angular distributions. Our results represent an important step towards going beyond band structure (eigenvalues) mapping and learning about electronic wavefunction and orbital texture of solids by exploiting matrix element effects in photoemission spectroscopy.
Simultaneous NAD(P)H and FAD fluorescence lifetime microscopy of long UVA–induced metabolic stress in reconstructed human skin
Authors: Ung Thi Phuong Lien , Lim Seongbin , Solinas Xavier , Mahou Pierre , Chessel Anatole , Marionnet Claire , Bornschlögl Thomas , Beaurepaire Emmanuel , Bernerd Françoise , Pena Ana-Maria , Stringari Chiara 
Published: November 2021

AbstractSolar ultraviolet longwave UVA1 exposure of human skin has short-term consequences at cellular and molecular level, leading at long-term to photoaging. Following exposure, reactive oxygen species (ROS) are generated, inducing oxidative stress that might impair cellular metabolic activity. However, the dynamic of UVA1 impact on cellular metabolism remains unknown because of lacking adequate live imaging techniques. Here we assess the UVA1-induced metabolic stress response in reconstructed human skin with multicolor two-photon fluorescence lifetime microscopy (FLIM). Simultaneous imaging of nicotinamide adenine dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD) by wavelength mixing allows quantifying cellular metabolism in function of NAD(P)+/NAD(P)H and FAD/FADH2redox ratios. After UVA1 exposure, we observe an increase of fraction of bound NAD(P)H and decrease of fraction of bound FAD indicating a metabolic switch from glycolysis to oxidative phosphorylation or oxidative stress possibly correlated to ROS generation. NAD(P)H and FAD biomarkers have unique temporal dynamic and sensitivity to skin cell types and UVA1 dose. While the FAD biomarker is UVA1 dose-dependent in keratinocytes, the NAD(P)H biomarker shows no dose dependence in keratinocytes, but is directly affected after exposure in fibroblasts, thus reflecting different skin cells sensitivities to oxidative stress. Finally, we show that a sunscreen including a UVA1 filter prevents UVA1 metabolic stress response from occurring.
A phase II study of talimogene laherparepvec for patients with inoperable locoregional recurrence of breast cancer
Authors: Kai Megumi , Marx Angela N. , Liu Diane D. , Shen Yu , Gao Hui , Reuben James M. , Whitman Gary , Krishnamurthy Savitri , Ross Merrick I. , Litton Jennifer K. , Lim Bora , Ibrahim Nuhad , Kogawa Takahiro , Ueno Naoto T. 
Published: November 2021

AbstractTalimogene laherparepvec (T-VEC) is an immunotherapy that generates local tumor lysis and systemic antitumor immune response. We studied the efficacy of intratumoral administration of T-VEC as monotherapy for inoperable locoregional recurrence of breast cancer. T-VEC was injected intratumorally at 106 PFU/mL on day 1 (cycle 1), 108 PFU/mL on day 22 (cycle 2), and 108 PFU/mL every 2 weeks thereafter (cycles ≥ 3). Nine patients were enrolled, 6 with only locoregional disease and 3 with both locoregional and distant disease. No patient completed the planned 10 cycles or achieved complete or partial response. The median number of cycles administered was 4 (range, 3–8). Seven patients withdrew prematurely because of uncontrolled disease progression, 1 withdrew after cycle 3 because of fatigue, and 1 withdrew after cycle 4 for reasons unrelated to study treatment. Median progression-free survival and overall survival were 77 days (95% CI, 63–NA) and 361 days (95% CI, 240–NA). Two patients received 8 cycles with clinically stable disease as the best response. The most common grade 2 or higher adverse event was injection site reaction (n = 7, 78%). Future studies could examine whether combining intratumoral T-VEC with concurrent systemic therapy produces better outcomes.
Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
Authors: Shahid Syed Salman , Johnston Robert D. , Smekens Celine , Kerskens Christian , Gaul Robert , Tornifoglio Brooke , Stone Alan J. , Lally Caitríona 
Published: November 2021

AbstractThe purpose of this study was to characterize the alterations in microstructural organization of arterial tissue using higher-order diffusion magnetic resonance schemes. Three porcine carotid artery models namely; native, collagenase treated and decellularized, were used to estimate the contribution of collagen and smooth muscle cells (SMC) on diffusion signal attenuation using gaussian and non-gaussian schemes. The samples were imaged in a 7 T preclinical scanner. High spatial and angular resolution diffusion weighted images (DWIs) were acquired using two multi-shell (max b-value = 3000 s/mm2) acquisition protocols. The processed DWIs were fitted using monoexponential, stretched-exponential, kurtosis and bi-exponential schemes. Directionally variant and invariant microstructural parametric maps of the three artery models were obtained from the diffusion schemes. The parametric maps were used to assess the sensitivity of each diffusion scheme to collagen and SMC composition in arterial microstructural environment. The inter-model comparison showed significant differences across the considered models. The bi-exponential scheme based slow diffusion compartment (Ds) was highest in the absence of collagen, compared to native and decellularized microenvironments. In intra-model comparison, kurtosis along the radial direction was the highest. Overall, the results of this study demonstrate the efficacy of higher order dMRI schemes in mapping constituent specific alterations in arterial microstructure.
Discovery proteomics defines androgen-regulated glycoprotein networks in prostate cancer cells, as well as putative biomarkers of prostatic diseases
Authors: Hsiao Jordy J. , Smits Melinda M. , Ng Brandon H. , Lee Jinhee , Wright Michael E. 
Published: November 2021

AbstractSupraphysiologic androgen (SPA) inhibits cell proliferation in prostate cancer (PCa) cells by transcriptional repression of DNA replication and cell-cycle genes. In this study, quantitative glycoprotein profiling identified androgen-regulated glycoprotein networks associated with SPA-mediated inhibition of PCa cell proliferation, and androgen-regulated glycoproteins in clinical prostate tissues. SPA-regulated glycoprotein networks were enriched for translation factors and ribosomal proteins, proteins that are known to beO-GlcNAcylated in response to various cellular stresses. Thus, androgen-regulated glycoproteins are likely to be targeted forO-GlcNAcylation. Comparative analysis of glycosylated proteins in PCa cells and clinical prostate tissue identified androgen-regulated glycoproteins that are differentially expressed prostate tissues at various stages of cancer. Notably, the enzyme ectonucleoside triphosphate diphosphohydrolase 5 was found to be an androgen-regulated glycoprotein in PCa cells, with higher expression in cancerous versus non-cancerous prostate tissue. Our glycoproteomics study provides an experimental framework for characterizing androgen-regulated proteins and glycoprotein networks, toward better understanding how this subproteome leads to physiologic and supraphysiologic proliferation responses in PCa cells, and their potential use as druggable biomarkers of dysregulated AR-dependent signaling in PCa cells.
Sensorimotor strategy selection under time constraints in the presence of two motor targets with different values
Authors: Onagawa Ryoji , Kudo Kazutoshi 
Published: November 2021

AbstractGoal-directed movements often require choosing an option from multiple potential goals under time constraints. However, there are limited studies on how humans change their time spent on decision-making and movement patterns according to time constraints. Here, we examined how sensorimotor strategies are selected under time constraints when the target values are uncertain. In the double-target condition, the values were uncertain until the movement onset and presented immediately afterwards. The behavior in this condition was compared to the single-target condition, in relation to time constraints and target-separation-angles. The results showed that the participants frequently used the choice-reaction even under tight time constraints, and their performance was consistently lower than that in the single-target condition. Additionally, in the double-target condition, differences in the movement trajectory depending on the time constraint and target-separation angle were confirmed. Specifically, the longer the time constraint, the higher the frequency of the intermediate behavior (to initiate movement toward the intermediate direction of two targets) or the change-of-mind behavior (to change the aiming target during movement). Furthermore, the smaller the target-separation angle, the higher the frequency of intermediate behavior, but the frequency of change-of-mind was not affected by the target-separation angle. These results suggest that the participants initiated the movement at an incomplete value judgment stage in some trials. Furthermore, they seemed to select a strategy to utilize the information obtained during the movement, taking into account the time constraints and target-separation angle. Our results show a consistent cognitive bias in choosing a higher value when multiple alternatives have different values. Additionally, we also suggest flexibility and adaptability in the movement patterns in response to time constraints.
Long-term straw returning improve soil K balance and potassium supplying ability under rice and wheat cultivation
Authors: Zhang Zhiyi , Liu Dongbi , Wu Maoqian , Xia Ying , Zhang Fulin , Fan Xianpeng 
Published: November 2021

AbstractThe aims of the present study were to provide scientific bases for rational use of crop straw to substitute chemical potassium (K) input. The effects of potassium fertilization and straw incorporation on soil K balance and K supplying in a long-term (14 years) field experiment. Five treatments were examined: (1) no fertilization (CK); (2) mineral fertilizing (NPK); (3) straw 6000 kg h m−2 (S); (4) NPK with straw 3000 kg h m−2 (NPK1/2S); and (5) NPK with straw 6000 kg h m−2 (NPKS). K composition, K balance and quantity-intensity (Q/I) relationship were studied. Under no fertilization or low straw returned conditions, soil K was unbalanced and deficienct seriously. Straw return at 6000 kg h m−2 per season with fertilization improved the soil potassium supply and K balance. Long-term K surplus (4 or 5 years), compared with NPK, the NPKS significantly increased non-special K adsorption (Knsa) and non-exchangeable K (Kne) by 5.7–11.2 mg kg−1 and 65.7–128.1 mg kg−1, respectively. Q/I relationship showed cropping without straw K or without fertilizer K resulted in lower quantity (nonspecifically and specifically held K i.e. – ∆K0 and Kx) and intensity (equilibrium activity ratio i.e. CR0K) of K in tested soils. K-fertilization with straw maintain higher exchangeable K (EK0) and a higher difference between EK0 and minimum exchangeable K(EKmin), and would help to prevent depletion in non-exchangeable pool of soil K under intensive cropping. Additionally, The straw return mainly decreased potential buffering capacity for exchangeable pool (PBCKn), 43.92–48.22% of added K in soil might be converted to exchangeable pool while it was 25.67–29.19% be converted to non-exchangeable pool. The contribution of exchangeable K towards plant K uptake would be higher in the soil with straw than the soil without straw and the non-exchangeable K would be the long-term fixed K as a supplement to the potassium pool. K fertilizer with 6000 kg h m−2 straw return in each crop season increased soil available K and slowly available K. The findings underlined importance of the straw return and contribution for sustain K supplying ability of soils.
Non-classical nucleation in vapor–liquid–solid growth of monolayer WS2 revealed by in-situ monitoring chemical vapor deposition
Authors: Qiang Xiaoming , Iwamoto Yuta , Watanabe Aoi , Kameyama Tomoya , He Xing , Kaneko Toshiro , Shibuta Yasushi , Kato Toshiaki 
Published: November 2021

AbstractThe very early nucleation stage of a transition metal dichalcogenide (TMD) was directly observed with in-situ monitoring of chemical vapor deposition and automated image analysis. Unique nucleation dynamics, such as very large critical nuclei and slow to rapid growth transitions, were observed during the vapor–liquid–solid (VLS) growth of monolayer tungsten disulfide (WS2). This can be explained by two-step nucleation, also known as non-classical nucleation, in which metastable clusters are formed through the aggregation of droplets. Subsequently, nucleation of solid WS2 takes place inside the metastable cluster. Furthermore, the detailed nucleation dynamics was systematically investigated from a thermodynamic point of view, revealing that the incubation time of metastable cluster formation follows the traditional time–temperature transformation diagram. Quantitative phase field simulation, combined with Bayesian inference, was conducted to extract quantitative information on the growth dynamics and crystal anisotropy from in-situ images. A clear transition in growth dynamics and crystal anisotropy between the slow and rapid growth phases was quantitatively verified. This observation supports the existence of two-step nucleation in the VLS growth of WS2. Such detailed understanding of TMD nucleation dynamics can be useful for achieving perfect structure control of TMDs.
Alterations of the 70 kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
Authors: Orfanelli Theofano , Giannopoulos Spyridon , Zografos Eleni , Athanasiou Aikaterini , Bongiovanni Ann Marie , Doulaveris Georgios , Moo Tracy-Ann , LaPolla Dayle , Bakoyiannis Chris N. , Theodoropoulos Georgios E. , Zografos Georgios C. , Andreopoulou Eleni , Witkin Steven S. 
Published: November 2021

AbstractPeripheral blood mononuclear cells (PBMCs) respond to altered physiological conditions to alleviate the threat. Production of the 70 kDa heat shock protein (HSP70) is up-regulated to protect proteins from degradation. Sequestosome-1 (p62) binds to altered proteins and the p62-protein complex is degraded by autophagy. P62 is also a regulator of intracellular kinase activity and cell differentiation. We hypothesized that the PBMC response to a malignant breast mass involves elevated production of HSP70 and a decrease in intracellular p62. In this study 46 women had their breast mass excised. PBMCs were isolated and intracellular levels of HSP70 and p62 were quantitated by ELISA. Differences between women with a benign or malignant breast mass were determined. A breast malignancy was diagnosed in 38 women (82.6%) while 8 had a benign lesion. Mean intracellular HSP70 levels were 79.3 ng/ml in PBMCs from women with a malignant lesion as opposed to 44.2 ng/ml in controls (p = 0.04). The mean PBMC p62 level was 2.3 ng/ml in women with a benign breast lesion as opposed to 0.6 ng/ml in those with breast cancer (p < 0.001). Mean p62 levels were lowest in women with invasive carcinoma and a positive lymph node biopsy when compared to those with in-situ carcinoma or absence of lymphadenopathy, respectively. Intracellular HSP70 and p62 levels in PBMCs differ between women with a malignant or benign breast lesion. These measurements may be of value in the preoperative triage of women with a breast mass.
Whole blood transcriptomic analysis reveals PLSCR4 as a potential marker for vaso-occlusive crises in sickle cell disease
Authors: Abdulwahab Hawra , Aljishi Muna , Sultan Ameera , Al-Kafaji Ghada , Sridharan Kannan , Bakhiet Moiz , Taha Safa 
Published: November 2021

AbstractSickle cell disease, a common genetic blood disorder, results from a point mutation in the β-globin gene affecting the configuration of hemoglobin, predisposing to painful vaso-occlusive crisis (VOC) and multi-organ dysfunctions. There is a huge variation in the phenotypic expressions of SCD and VOC owing to genetic and environmental factors. This study aimed to characterize the whole blood gene expression profile using Microarray technology in Bahraini patients with SCD determining the differentially expressed genes in steady-state (n = 10) and during VOC (n = 10) in comparison to healthy controls (n = 8). Additionally, the study intended to identify potential genetic marker associated with hemolysis. The analysis identified 2073 and 3363 genes that were dysregulated during steady-state and VOC, respectively, compared to healthy controls. Moreover, 1078 genes were differentially expressed during VOC compared to steady state. The PLSCR4 gene was almost 6-fold up-regulated in microarray, 4-fold in polymerase chain reaction, and a mean protein concentration of 0.856 ng/ml was observed in enzyme-linked immunosorbent assay during VOC compared to steady-state (0.238 ng/ml) (p < 0.01). Amongst these genes, PLSCR4 is involved in erythrocyte membrane deformity thus, predisposing to hemolysis, adhesion, and thrombosis. In conclusion, PLSCR4 may serve as a potential biomarker for VOC and future large-scale validation are recommended.
Electric field-induced crystallization of ferroelectric hafnium zirconium oxide
Authors: Lederer Maximilian , Abdulazhanov Sukhrob , Olivo Ricardo , Lehninger David , Kämpfe Thomas , Seidel Konrad , Eng Lukas M. 
Published: November 2021

AbstractFerroelectricity in crystalline hafnium oxide thin films is strongly investigated for the application in non-volatile memories, sensors and other applications. Especially for back-end-of-line (BEoL) integration the decrease of crystallization temperature is of major importance. However, an alternative method for inducing ferroelectricity in amorphous or semi-crystalline hafnium zirconium oxide films is presented here, using the newly discovered effect of electric field-induced crystallization in hafnium oxide films. When applying this method, an outstanding remanent polarization value of 2P$$_{\mathrm{R}}$$

R

= 47 $$\upmu$$
μ
C/cm$$^{2}$$

2

is achieved for a 5 nm thin film. Besides the influence of Zr content on the film crystallinity, the reliability of films crystallized with this effect is explored, highlighting the controlled crystallization, excellent endurance and long-term retention.
Evaluation of the Apple iPhone 12 Pro LiDAR for an Application in Geosciences
Authors: Luetzenburg Gregor , Kroon Aart , Bjørk Anders A. 
Published: November 2021

AbstractTraditionally, topographic surveying in earth sciences requires high financial investments, elaborate logistics, complicated training of staff and extensive data processing. Recently, off-the-shelf drones with optical sensors already reduced the costs for obtaining a high-resolution dataset of an Earth surface considerably. Nevertheless, costs and complexity associated with topographic surveying are still high. In 2020, Apple Inc. released the iPad Pro 2020 and the iPhone 12 Pro with novel build-in LiDAR sensors. Here we investigate the basic technical capabilities of the LiDAR sensors and we test the application at a coastal cliff in Denmark. The results are compared to state-of-the-art Structure from Motion Multi-View Stereo (SfM MVS) point clouds. The LiDAR sensors create accurate high-resolution models of small objects with a side length > 10 cm with an absolute accuracy of ± 1 cm. 3D models with the dimensions of up to 130 × 15 × 10 m of a coastal cliff with an absolute accuracy of ± 10 cm are compiled. Overall, the versatility in handling outweighs the range limitations, making the Apple LiDAR devices cost-effective alternatives to established techniques in remote sensing with possible fields of application for a wide range of geo-scientific areas and teaching.