bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Auditory–Oral Education on Device Use in Children With Hearing Loss
Authors: Sanchez Chrisanda , Coto Jennifer , Berrios Daniela , Cejas Ivette 
Published: December 2021

Purpose:
This study examined changes in datalogging for children attending an auditory–oral educational program with integrated audiology services versus children attending a mainstream or nonspecialized program.

Method:
Eighty children participated in this study, half of which were enrolled in an auditory–oral educational program versus the nonspecialized or mainstream setting. Datalogging for cochlear implant and hearing aid users was obtained via retrospective medical and educational chart review from 2016 to 2019.

Results:
Results demonstrated that at post-enrollment, children attending the auditory–oral educational program significantly increased device wear time (as measured by average hours/day) when compared to the control group. Children using hearing aids enrolled in the specialized educational program obtained the largest improvement in overall wear time, averaging an increase of 5 hr/day of device use from pre- to post-enrollment.

Conclusions:
This is the first study to document the association of specialized educational programs on device use. Clinical and educational programs should collaborate to provide integrated services to lessen family burden and increase a child's device use and retention.
Practical Approach to Hair Loss Diagnosis
Authors: Singh Sukhbir , Muthuvel Kumaresan 
Published: December 2021

AbstractHair loss is one of the common complaints encountered in a cosmetic practice. Evaluating a patient with hair loss requires a thorough understanding of the various causes of hair loss and practical knowledge in hair cosmetics and hair styling procedures. A systematic approach in hair loss evaluation helps the treating physician in arriving at a diagnosis. Various bedside tests and advent of trichoscopy has empowered the physician in arriving at a diagnosis. This article aims to provide the physician a systematic approach in evaluating a patient with hair loss.
Real-time, single-molecule observation of biomolecular interactions inside nanophotonic zero mode waveguides
Authors: Nemashkalo A , Phipps M E , Hennelly S P , Goodwin P M 
Published: December 2021

Abstract
Living cells rely on numerous protein-protein, RNA-protein and DNA-protein interactions for processes such as gene expression, biomolecular assembly, protein and RNA degradation. Single-molecule microscopy and spectroscopy are ideal tools for real-time observation and quantification of nucleic acids-protein and protein-protein interactions. One of the major drawbacks of conventional single-molecule imaging methods is low throughput. Methods such as sequencing by synthesis utilizing nanofabrication and single-molecule spectroscopy have brought high throughput into the realm of single-molecule biology. The Pacific Biosciences RS2 sequencer utilizes sequencing by synthesis within nanophotonic zero mode waveguides. A number of years ago this instrument was unlocked by Pacific Biosciences for custom use by researchers allowing them to monitor biological interactions at the single-molecule level with high throughput. In this capability letter we demonstrate the use of the RS2 sequencer for real-time observation of DNA-to-RNA transcription and RNA-protein interactions. We use a relatively complex model–transcription of structured ribosomal RNA from E. coli and interactions of ribosomal RNA with ribosomal proteins. We also show evidence of observation of transcriptional pausing without the application of an external force (as is required for single-molecule pausing studies using optical traps). Overall, in the unlocked, custom mode, the RS2 sequencer can be used to address a wide variety of biological assembly and interaction questions at the single-molecule level with high throughput. This instrument is available for use at the Center for Integrated Nanotechnologies Gateway located at Los Alamos National Laboratory.
Green public procurement: learnings from Pesaro city, Italy
Authors: Iyer-Raniga Usha , Finamore Margherita 
Published: December 2021

Abstract
The development of mandatory green public procurement requirements is a significant and tangible contribution to undertaking planned, sustainable deconstruction (rather than demolition) approach to increase recycling materials, creating new businesses and new green jobs. Research undertaken of a procurement process for Pesaro, Italy demonstrates the application of an assessment approach and method based on holistic evaluation and interpretation of multiple sustainability aspects of buildings and their parts. Taking a holistic life cycle approach, this case study provides a clear evidence of how to foster ‘circular’ alternatives compared to linear, business as usual options in the management of public procurement. A competitive procedure in the procurement process was undertaken resulting in materials either being reused or taken to a recycling centre. The reuse of the materials during the new construction phase also included monitoring the quantity of all materials, preventing any waste and encouraging optimised reuse. This study shows that it is possible to drive the market towards a circular thinking and a circular construction site management is a critical requirement for transitioning towards a circular built environment. By ensuring that tendering processes support and develop sustainable recovery of waste materials, this study shows that it is possible to provide materials second and third life.
Circularity Assessment using the UN 2030 Agenda Indicators
Authors: Huovila P , Iyer-Raniga U 
Published: December 2021

Abstract
Established in 2015, the One Planet Sustainable Buildings and Construction (SBC) Programme has studied the state of play for circular built environment in different regions: Africa, Asia, Europe, Latin America, Middle East, North America and Oceania. One aspect of this study has been assessment for circularity using the 2030 Agenda indicators. This paper describes how nature’s resources are used in buildings to provide more comfortable and healthier spaces while generating less waste and emissions with the aim to change linear processes to circular, thereby increasing resource efficiency and reducing waste and emissions, while also providing local jobs.
A set of core indicators have been selected to assess the circularity at global and regional levels. It is intended that the indicators selected for SBC become a starting point for prioritising the built environment sector to embrace circularity principles and practices.
Facilitating circular site preparation; developing the instrument PrCiSi
Authors: Entrop A G , Hagen L , Kuiper A M 
Published: December 2021

Abstract
The urge to strive for closed material loops is felt in the construction industry. However, before the built environment can be regarded as circular, not only the material usage in buildings, but also preparations taken on sites need to be circular. In this paper a new instrument is presented to help project leaders at municipalities and contractors to assess how circular their process of site preparation is. The instrument, by the name of PrCiSi, has been developed on basis of a literature study on sustainable material usage and interviews among stakeholders in this niche market. The instrument has been tested on the site preparation processes for the residential neighbourhoods Olstergaard and Noorder Koeslag in the municipality of Olst-Wijhe. Of course, there is still room for improvement and more test cases are welcomed, but three main subjects, namely materials, equipment and personnel, seem to form a proper basis. A large number of test cases might help us to further develop the scoring system. However, being convinced that we can cross a boundary here, we are happy to share with you the current status to inspire you to use and improve this instrument with us.
Structural color coatings for high performance BIPV
Authors: Habets R , Vroon Z , Erich B , Meulendijks N , Mann D , Buskens P 
Published: December 2021

Abstract
Building integrated photovoltaics (BIPV) offer aesthetics and freedom of design for architects and home owners. This can accelerate implementation and free up new spaces for solar energy harvesting at building level, which is a necessary step towards a climate neutral built environment. Colored solar panels with high conversion efficiency and low cost price are an important development for large scale market penetration of BIPV. Here we report a solution processed structural color coating for solar panels and solar collectors. We show that virtually any color can be prepared, that the desired coating stack can be designed using optical calculations and that the exact color can be produced via a low cost solution process. Furthermore, we show that the light transmission for the colored glass plates is still very high, exceeding commonly used absorbing colors and enables very high solar cell efficiency. The colored PV panels have been tested in real environment and via accelerated lifetime testing for 3 years without any performance decline or degradation.
CO-CREATING SYSTEMIC CHANGES FOR A CIRCULAR ECONOMY IN/UNDER CONSTRUCTION, PRELIMINARY LESSONS FROM THE FLEMISH LIVING LAB ON CIRCULAR CONSTRUCTION
Authors: Galle Waldo , Debacker Wim , Weerdt Yves De 
Published: December 2021

Abstract
Like any persistent challenge, the transition from a linear to a circular construction economy requires systemic changes. To divert from current practices that maintain the take-make-waste logic, we need new ways of doing and thinking within the value network of construction. In search for those enduring changes, a compass group advises the Flemish Living Lab on Circular Construction. This lab is a three-and-a-half-year project of study and experiment initiated by policy. During four participatory workshops, the compass group identified the most important system hurdles and necessary changes for achieving a circular construction economy, through subsequent steps of system analysis, translation and reflection. These outcomes have been synthesized and validated during the last workshop and are presented in this paper as a preliminary outcome of lab. Further, by bringing together forerunners of construction and co-creating together new knowledge, it is demonstrated how a well-guided compass group allows to bring into practice ‘participatory system modelling’. By consequently translating the resulting insights into the requirements for a targeted call for experiments, it is finally shown that this co-created knowledge has been ‘actionable’ for the policy makers of Circular Flanders, and triggered dozens of partnerships to submit an experiment.
A brief review: effects of resonant magnetic perturbation on classical and neoclassical tearing modes in tokamaks
Authors: WANG Zhengxiong , TANG Weikang , WEI Lai 
Published: December 2021

Abstract
This paper reviews the effects of resonant magnetic perturbation (RMP) on classical tearing modes (TMs) and neoclassical tearing modes (NTMs) from the theory, experimental discovery and numerical results with a focus on four major aspects: (i) mode mitigation, where the TM/NTM is totally suppressed or partly mitigated by the use of RMP; (ii) mode penetration, which means a linearly stable TM/NTM triggered by the externally applied RMP; (iii) mode locking, namely an existing rotating magnetic island braked and finally stopped by the RMP; (iv) mode unlocking, as the name suggests, it is the reverse of the mode locking process. The key mechanism and physical picture of above phenomena are revealed and summarized.
Protein turnover models for LC–MS data of heavy water metabolic labeling
Authors: Sadygov Rovshan G 
Published: December 2021

AbstractProtein turnover is vital for cellular functioning and is often associated with the pathophysiology of a variety of diseases. Metabolic labeling with heavy water followed by liquid chromatography coupled to mass spectrometry is a powerful tool to study in vivo protein turnover in high throughput and large scale. Heavy water is a cost-effective and easy to use labeling agent. It labels all nonessential amino acids. Due to its toxicity in high concentrations (20% or higher), small enrichments (8% or smaller) of heavy water are used with most organisms. The low concentration results in incomplete labeling of peptides/proteins. Therefore, the data processing is more challenging and requires accurate quantification of labeled and unlabeled forms of a peptide from overlapping mass isotopomer distributions. The work describes the bioinformatics aspects of the analysis of heavy water labeled mass spectral data, available software tools and current challenges and opportunities.
CharID: a two-step model for universal prediction of interactions between chromatin accessible regions
Authors: Shen Yin , Zhong Quan , Liu Tian , Wen Zi , Shen Wei , Li Li 
Published: December 2021

Abstract
Open chromatin regions (OCRs) allow direct interaction between cis-regulatory elements and trans-acting factors. Therefore, predicting all potential OCR-mediated loops is essential for deciphering the regulation mechanism of gene expression. However, existing loop prediction tools are restricted to specific anchor types. Here, we present CharID (Chromatin Accessible Region Interaction Detector), a two-step model that combines neural network and ensemble learning to predict OCR-mediated loops. In the first step, CharID-Anchor, an attention-based hybrid CNN-BiGRU network is constructed to discriminate between the anchor and nonanchor OCRs. In the second step, CharID-Loop uses gradient boosting decision tree with chromosome-split strategy to predict the interactions between anchor OCRs. The performance was assessed in three human cell lines, and CharID showed superior prediction performance compared with other algorithms. In contrast to the methods designed to predict a particular type of loops, CharID can detect varieties of chromatin loops not limited to enhancer–promoter loops or architectural protein-mediated loops. We constructed the OCR-mediated interaction network using the predicted loops and identified hub anchors, which are highlighted by their proximity to housekeeping genes. By analyzing loops containing SNPs associated with cardiovascular disease, we identified an SNP–gene loop indicating the regulation mechanism of the GFOD1. Taken together, CharID universally predicts diverse chromatin loops beyond other state-of-the-art methods, which are limited by anchor types, and experimental techniques, which are limited by sensitivities drastically decaying with the genomic distance of anchors. Finally, we hosted Peaksniffer, a user-friendly web server that provides online prediction, query and visualization of OCRs and associated loops.
Is the mode elicitable relative to unimodal distributions?
Authors: Heinrich-Mertsching Claudio , Fissler Tobias 
Published: December 2021

Summary
A statistical functional is said to be elicitable if there exists a loss or scoring function under which the functional is the optimal point forecast in expectation. While the mean and quantiles are elicitable, it has been shown in Heinrich (2014) that the mode is not elicitable if the true distribution can follow any Lebesgue density. We strengthen the result of Heinrich (2014) substantially, showing that the mode is not elicitable if the true distribution can be any strongly unimodal distribution with continuous Lebesgue density, i.e., a continuous density with only one local maximum. Likewise, the mode fails to be identifiable relative to this class.
Determination of Potential Genotoxic Impurities (Orthophenylene Diamine Dihydrochloride), 4’-Bromomethyl-2-Cyanobiphenyl, 4’(Dibromomethyl)[1,1’-Biphenyl]-2-Carbonitrile in Telmisartan by ESI-MS/MS
Authors: Yarala Manikanth Reddy , Gummadi Sowjanya 
Published: December 2021

AbstractA sensitive, simple, rapid and robust LC–MS/MS method was developed for the determination of potential genotoxic impurities OPDA HCl (orthophenylene diamine dihydrochloride), bromo OTBN (4’-bromomethyl-2-cyanobiphenyl), dibromo (4’(dibromomethyl)[1,1′-biphenyl]-2-carbonitrile) in Telmisartan by using ESI-MS/MS Technic; the study was performed with gradient elution (time/% mobile phase B: 0/10, 3/10, 30/80, 35/80, 36/10, 40/10). The mobile phase consisted of a mixture of formic acid, methanol and acetonitrile. The buffer was degassed before running at a flow rate of 1.0 mL/min. The column temperature was at 40°C. The 20 μL volume of sample was injected per run and peaks were detected using DAD detector. The LC-ESI/MS/MS studies were carried out on Ultivo Triple Quadrupole LC/MS/MS (Agilent, USA) G6470A mass spectrometer, ion source voltage 3500 V, de clustering potential 40 V, entrance potential 10 V, with the nebulizer gas as nitrogen at 45 psi. The LC part consisted of Agilent 1260 series HPLC system with binary gradient pump with a degasser and an auto sampler. Inert sustain AQ-C18, 250 × 4.6 mm, 5-μm particle size was used for chromatographic separation. Developed method is validated as per ICH guidelines and found to be linear, accurate, specific, selective, precise and robust. Test solution was found to be stable up to 48 h. This method can be successfully applied for the determination of genotoxic impurities in Telmisarta for routine analysis and stability assessment.
Qiviut cortisol is associated with metrics of health and other intrinsic and extrinsic factors in wild muskoxen (Ovibos moschatus)
Authors: Di Francesco Juliette , Kwong Grace P S , Deardon Rob , Checkley Sylvia L , Mastromonaco Gabriela F , Mavrot Fabien , Leclerc Lisa-Marie , Kutz Susan 
Published: December 2021

Abstract
Glucocorticoid (GC) levels are increasingly and widely used as biomarkers of hypothalamic–pituitary–adrenal (HPA) axis activity to study the effects of environmental changes and other perturbations on wildlife individuals and populations. However, identifying the intrinsic and extrinsic factors that influence GC levels is a key step in endocrinology studies to ensure accurate interpretation of GC responses. In muskoxen, qiviut (fine woolly undercoat hair) cortisol concentration is an integrative biomarker of HPA axis activity over the course of the hair’s growth. We gathered data from 219 wild muskoxen harvested in the Canadian Arctic between October 2015 and May 2019. We examined the relationship between qiviut cortisol and various intrinsic (sex, age, body condition and incisor breakage) and extrinsic biotic factors (lungworm and gastrointestinal parasite infections and exposure to bacteria), as well as broader non-specific landscape and temporal features (geographical location, season and year). A Bayesian approach, which allows for the joint estimation of missing values in the data and model parameters estimates, was applied for the statistical analyses. The main findings include the following: (i) higher qiviut cortisol levels in males than in females; (ii) inter-annual variations; (iii) higher qiviut cortisol levels in a declining population compared to a stable population; (iv) a negative association between qiviut cortisol and marrow fat percentage; (v) a relationship between qiviut cortisol and the infection intensity of the lungworm Umingmakstrongylus pallikuukensis, which varied depending on the geographical location; and (vi) no association between qiviut cortisol and other pathogen exposure/infection intensity metrics. This study confirmed and further identified important sources of variability in qiviut cortisol levels, while providing important insights on the relationship between GC levels and pathogen exposure/infection intensity. Results support the use of qiviut cortisol as a tool to monitor temporal changes in HPA axis activity at a population level and to inform management and conservation actions.
SCANNER: a web platform for annotation, visualization and sharing of single cell RNA-seq data
Authors: Cai Guoshuai , Yu Xuanxuan , Youn Choonhan , Zhou Jun , Xiao Feifei 
Published: December 2021

AbstractIn recent years, efficient scRNA-seq methods have been developed, enabling the transcriptome profiling of single cells massively in parallel. Meanwhile, its high dimensionality and complexity bring challenges to the data analysis and require extensive collaborations between biologists and bioinformaticians and/or biostatisticians. The communication between these two units demands a platform for easy data sharing and exploration. Here we developed Single-Cell Transcriptomics Annotated Viewer (SCANNER), as a public web resource for the scientific community, for sharing and analyzing scRNA-seq data in a collaborative manner. It is easy-to-use without requiring special software or extensive coding skills. Moreover, it equipped a real-time database for secure data management and enables an efficient investigation of the activation of gene sets on a single-cell basis. Currently, SCANNER hosts a database of 19 types of cancers and COVID-19, as well as healthy samples from lungs of smokers and non-smokers, human brain cells and peripheral blood mononuclear cells (PBMC). The database will be frequently updated with datasets from new studies. Using SCANNER, we identified a larger proportion of cancer-associated fibroblasts cells and more active fibroblast growth-related genes in melanoma tissues in female patients compared to male patients. Moreover, we found ACE2 is mainly expressed in lung pneumocytes, secretory cells and ciliated cells and differentially expressed in lungs of smokers and never smokers.
Heart failure with preserved ejection fraction in patients with normal natriuretic peptide levels is associated with increased morbidity and mortality
Authors: Verbrugge Frederik H , Omote Kazunori , Reddy Yogesh N V , Sorimachi Hidemi , Obokata Masaru , Borlaug Barry A 
Published: December 2021

Abstract

Background
A substantial proportion of patients with heart failure (HF) with preserved ejection fraction (HFpEF) present with normal natriuretic peptide (NP) levels. The pathophysiology and natural history for this phenotype remain unclear.

Methods and results
Consecutive subjects undergoing invasive cardiopulmonary exercise testing for unexplained dyspnoea at Mayo Clinic in 2006–18 were studied. Heart failure with preserved ejection fraction was defined as a pulmonary arterial wedge pressure (PAWP) ≥15 mmHg (rest) or ≥25 mmHg (exercise). Patients with HFpEF and normal NP [N-terminal of the pro-hormone B-type natriuretic peptide (NT-proBNP) &lt; 125 ng/L] were compared with HFpEF with high NP (NT-proBNP ≥ 125 ng/L) and controls with normal haemodynamics. Patients with HFpEF and normal (n = 157) vs. high NP (n = 263) were younger, yet older than controls (n = 161), with an intermediate comorbidity profile. Normal NP HFpEF was associated with more left ventricular hypertrophy and worse diastolic function compared with controls, but better diastolic function, lower left atrial volumes, superior right ventricular function, and less mitral/tricuspid regurgitation compared with high NP HFpEF. Cardiac output (CO) reserve with exercise was preserved in normal NP HFpEF [101% predicted, interquartile range (IQR): 75–124%], but this was achieved only at the cost of higher left ventricular transmural pressure (LVTMP) (14 ± 6 mmHg vs. 7 ± 4 mmHg in controls, P &lt; 0.001). In contrast, CO reserve was decreased in high NP HFpEF (85% predicted, IQR: 59–109%), with lower LVTMP (10 ± 8 mmHg) compared with normal NP HFpEF (P &lt; 0.001), despite similar PAWP. Patients with high NP HFpEF displayed the highest event rates, but normal NP HFpEF still had 2.7-fold higher risk for mortality or HF readmissions compared with controls (hazard ratio: 2.74, 95% confidence interval: 1.02–7.32) after adjusting for age, sex, and body mass index.

Conclusion
Patients with HFpEF and normal NP display mild diastolic dysfunction and preserved CO reserve during exercise, despite marked elevation in filling pressures. While clinical outcomes are not as poor compared with patients with high NP, patients with normal NP HFpEF exhibit increased risk of death or HF readmissions compared with patients without HF, emphasizing the importance of this phenotype.