bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health Status Transitions Related to Traumatic Brain Injury.
Authors: Escobar Michael , Mollayeva Tatyana , Tran Andrew , Chan Vincy , Colantonio Angela , Sutton Mitchell 
Published: May 2021

Abstract
For centuries, the study of traumatic brain injury (TBI) has been centred on historical observation and analyses of personal, social, and environmental processes, which have been examined separately. Today, however, computation implementation and vast patient data repositories are producing datasets of such volume and complexity as to challenge traditional scientific methodology. Drastically different approaches show great promise for research on TBI. We report a computational analysis of health status over time using population-wide health administrative data of patients with TBI. Our approach facilitates health status at injury event evaluation and classification, and unfolds health status trajectories, from that of preceding injury to the injury event itself, as they concern external causes of injury and injury severity. Taken together, the contrasting and interwoven aspects of health status on a time continuum can influence injury event trajectories and should be considered in TBI risk analysis for the improvement of diagnosis, treatment, and prevention.
Remimazolam protects against LPS-induced endotoxicity improving survival of endotoxemia mice
Authors: Liu Xiaolei , Lin Shaoping , Zhong Yiyue , Shen Jiaojiao , Zhang Xuedi , Luo Shuhua , Zhang Liangqing , Zhou Shuangnan , Tang Jing 
Published: May 2021

Abstract
Background: Remimazolam is a new benzodiazepine of sedative drugs with an ultra-short-acting anesthetic effect, being commonly used for critically ill patients (especially septic patients) in intensive care units (ICUs). Although some anesthetics have been reported to show certain anti-inflammatory effects, the role of remimazolam in inflammation is still remained unknown.Methods: In the present study, LPS-induced endotoxemia mice were used to observe the effect of remimazolam in vivo. LPS-stimulated bone marrow cells and Raw 264.7 cells were used to evaluate the influence of remimazolam on macrophage in vitro.Results: Compared with LPS treatment group, remimazolam remarkably improved survival rate of endotoxemia mice and decreased the release of LPS-induced inflammatory mediators, such as TNF-a, IL-6 and IL-1b. Remimazolam not only inhibited the activation of MyD88 and PI3K signal pathway at 15min after LPS treatment but also disturb Rab5a related TLR4 expression at cell surface in response to LPS at later time.Conclusions: Remimazolam inhibit LPS‑induced inflammatory responses of macrophages and obviously improve the survival rate of endotoxemia mice.
Compression of Traditional and Computer Face Emotion Recognition Training in Children with Autism, Clinical Trial Study
Authors: Bashirian Saeid , Khazaei Salman , Barati Majid , Mirzaei Fatemeh , Rezaei Mohammad , Jenabi Ensiyeh , Cheraghi Fatemeh , mohammadi fateme 
Published: May 2021

Abstract
Background

This study aimed to investigate the effect of traditional and computer emotional facial training on the recognition of facial emotional states in children with autism and mother –child relation and parental competence in mothers of autistic children.
Methods

The present clinical trial study was conducted in three childcare centers. In this study, children with high or moderate performance autism were selected by available sampling who were then assigned to the two groups using the random block sampling method. Thereafter, the children with autism in the both groups were trained during 10 sessions. In traditional group children training using 40 images related to four situations as happiness, sadness, anger, and fear. The children in the computer group trained with the Let's Face It software. These questionnaires were completed one day before and one day and one week after performing the intervention in both groups by assistant research. The obtained data were analyzed using SPSS software version 22.
Result

Findings showed that emotional state training has improved the identification of emotional states (p = 0.01), mother –child relation (p = 0.001) and parental competence (p = 0.001) in the both groups. There was also a statistically significant difference between the scores of identifying face emotional states (p = 0.01), mother –child relation (p = 0.001) and parental competence (p = 0.001) in the both groups. But training in traditional group was effectiveness than computer grope.
Conclusions

Teaching emotional face to children with autism improved the recognition of emotional states by these children, and subsequently improved the mother-child relationship and motherly sense of competence in the mothers of these children.
Platelet Membrane-Camouflaged Silver Metal-Organic Framework Drug System Against Infections Caused by Methicillin-Resistant Staphylococcus Aureus
Authors: Huang Rong , Cai Guang-Qing , Li Jian , Li Xi-Sheng , Liu Hai-Ting , Shang Xue-Ling , Zhou Jian-Dang , Nie Xin-Min , Gui Rong 
Published: May 2021

Abstract
Background
Due to the intelligent survival strategy and self-preservation of methicillin-resistant Staphylococcus aureus (MRSA), many antibiotics are ineffective in treating MRSA infections. Nano-drug delivery systems have emerged as a new method to overcome this barrier. The aim of this study was to construct a novel nano-drug delivery system for the treatment of MRSA infection, and to evaluate the therapeutic effect and biotoxicity of this system. We prepared a nano silver metal-organic framework using 2-methylimidazole as ligand and silver nitrate as ion provider. Vancomycin (Vanc) was loaded with Ag-MOF, and nano-sized platelet vesicles were prepared to encapsulate Ag-MOF-Vanc, thus forming the novel platelet membrane-camouflaged nanoparticles PLT@Ag-MOF-Vanc. The mice were infected with MRSA by tracheal injection to establish a model of pneumonia.
Results
The synthesized Ag-MOF particles had uniform size and shape of radiating corolla. The mean nanoparticle size and zeta potential of PLT@Ag-MOF-Vanc were 148 nm and − 25.6 mV, respectively. The encapsulation efficiency (EE) and loading efficiency (LE) of vancomycin were 81.0% and 64.7%, respectively. PLT@Ag-MOF-Vanc was shown to be a pH-responsive nano-drug delivery system with good biocompatibility. Ag-MOF had a good inhibitory effect on the growth of three common clinical strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus). PLT@Ag-MOF-Vanc showed better antibacterial activity against common clinical strains in vitro than vancomycin alone. PLT@Ag-MOF-Vanc killed MRSA through multiple approaches, including interfering with the metabolism of bacteria, catalyzing reactive oxygen species production, destroying the integrity of cell membrane, and inhibiting biofilm formation. PLT@Ag-MOF-Vanc targeted the MRSA infected sites and effectively reduced the dosage of vancomycin with no obvious toxicity.
Conclusions
PLT@Ag-MOF-Vanc is a novel effective targeted drug delivery system, which is expected to be used safely in anti-infective therapy of MRSA.
Performance Characterization of the SAFIR Prototype PET Insert
Authors: Khateri Parisa , Lustermann Werner , Ritzer Christian , Tsoumpas Charalampos , Dissertori Guenther 
Published: May 2021

Abstract
Background: The SAFIR prototype insert is a preclinical Positron Emission Tomography (PET) scanner built to acquire dynamic images simultaneously with a 7 T Bruker Magnetic Resonance Imaging (MRI) scanner. The insert is designed to perform with an excellent coincidence resolving time of 194 ps Full Width Half Maximum (FWHM) and an energy resolution of 13.8 FWHM. These properties enable it to acquire precise quantitative images at activities as high as 500 MBq suitable for studying fast biological processes within short time frames (<5 s). In this study, the performance of the SAFIR prototype insert is evaluated according to the NEMA NU 4-2008 standard while the insert is inside the MRI without acquiring MRI data.

Results: Applying an energy window of 391-601 keV and a coincidence time window of 500 ps the following results are achieved. The average spatial resolution at 5 mm radial offset is 2.6 mm FWHM when using the Filtered Backprojection 3D Reprojection (FBP3DRP) reconstruction method, improving to 2.3 mm when using the Maximum Likelihood Expectation Maximization (MLEM) method. The peak sensitivity at the center of the scanner is 1.06%. The Noise Equivalent Count Rate (NECR) is 799 kcps at the highest measured activity of 537 MBq for the mouse phantom and 121 kcps at the highest measured activity of 624 MBq for the rat phantom. The NECR peak is not yet reached for any of the measurements. The scatter fractions are 10.9% and 17.8% for the mouse and rat phantoms, respectively. The uniform region of the image quality phantom has a 3.0% STD, with a 4.6% deviation from the expected number of counts per voxel. The spill-over ratios for the water and air chambers are 0.18 and 0.17, respectively.

Conclusions: The results satisfy all the requirements initially considered for the insert, proving that the SAFIR prototype insert can obtain dynamic images of small rodents at high activities (~500 MBq) with a high sensitivity and an excellent count-rate performance.
Investigation of Vascular Endothelial Growth Factor (VEGF) Polymorphism in Patients With Idiopathic Heavy Menstrual Bleeding
Authors: Kaygusuz İkbal , Gündüz Nur Semerci 
Published: May 2021

Abstract
Purpose: To investigate whether there is a relationship between the VEGF gene polymorphisms and idiopathic heavy menstrual bleeding (HMB-E).Methods: 65 patients diagnosed with HMB-E according to the FIGO classification system and 65 female healty volunteers were included in the study. Detection of rs699947 (-2578C>A), rs1570360 (-1154G>A), rs2010963 (+405G>C), rs3025039 (+936C>T), rs25648 (c534C>T) polymorphic regions of the VEGF gene was performed by Next Generation DNA Sequencing method.Results: the -2578C>A polymorphic region CC, CA+AA genotypes, and C allele, as well as the -1154G>A polymorphic region AA genotype, and A allele were associated with increased risk of HMB-E (all p<0.05). However, no statistically significant difference was found between the the patient group and the control group in terms of genotype and allele distributions in the 405G>C, +936C>T, c534C>T polymorphic regions (all p>0.05). While the -2578/-1154/+405/c534 AGGC haplotype decreased the risk of HMB-E, CAGC haplotype was found to increase the risk of HMB-E.Conclusion: VEGF -2578C>A and -1154G>A polymorphisms were significantly associated with the risk of HMB-E in the Turkish population.
Damping Property of Carbon Fiber Reinforced Plastic for Noise/Vibration/Harsh of Steering Column Support Assembly
Authors: Ding Mingde , Liu Bo , Fan Zhenhua , Wang Jinyan , Zhai Fuqiang , Li Lu 
Published: May 2021

Abstract
Composite materials have penetrated into structural applications within the automotive industry, replacing traditional materials to reduce weight and then to improve fuel efficiency and to meet emission regulation. For some composite structural components, a significantly lower mode value compared to metal components can be accepted with an equivalent or better noise/vibration/harshness (NVH) performance, because, for composite materials, with different characteristics and much higher damping value compared to metal materials, a more sophisticated definition is required. An experimental investigation for mode definition of carbon fiber reinforced plastic (CFRP) steering column support assembly is discussed. Two kinds of composite steering column support assembly were manufactured, one is carbon fiber (CF) reinforced polyamide66 (PA66) with high mechanical properties, the other is glass fiber (GF) reinforced polyamide66 (PA66) with lower properties. The NVH test was carried out for these two components, and then the steering system mode requirement was defined by the test result. The new defined mode requirement obtained in the experiment showed good agreement with steering system mode analysis and test. By comparing the defined system mode requirement of composite component with that of the steel component, the effect of damping property of this composite material of the component can be clearly shown, which is over 21% percent on the NVH performance.
Radiolabeling and PET-MRI microdosing of the experimental cancer therapeutic, MN-anti-miR10b, demonstrates delivery to metastatic lesions in a murine model of metastatic breast cancer
Authors: Fur Mariane Le , Ross Alana , Pantazopoulos Pamela , Rotile Nicholas , Zhou Iris , Caravan Peter , Medarova Zdravka , Yoo Byunghee 
Published: May 2021

Abstract
Background
In our earlier work, we identified microRNA-10b (miR10b) as a master regulator of the viability of metastatic tumor cells. This knowledge allowed us to design a miR10b-targeted therapeutic consisting of anti-miR10b and ultrasmall iron oxide magnetic nanoparticles (MN), termed MN-anti-miR10b. In mouse models of breast cancer, we demonstrated that MN-anti-miR10b caused durable regressions of established metastases with no evidence of systemic toxicity. As a first step towards translating MN-anti-miR10b for the treatment of metastatic breast cancer, we needed to determine if MN-anti-miR10b, which is so effective in mice, will also accumulate in human metastases.
Results
In this study, we devised a method to efficiently radiolabel MN-anti-miR10b with Cu-64 (64Cu) and evaluated the pharmacokinetics and biodistribution of the radiolabeled product at two different doses: a therapeutic dose, referred to as macrodose, corresponding to 64Cu-MN-anti-miR10b co-injected with non-labeled MN-anti-miR10b, and a tracer level dose of 64Cu-MN-anti-miR10b, referred to as microdose. In addition, we evaluated the uptake of 64Cu-MN-anti-miR10b by metastatic lesions using both in vivo and ex vivo positron emission tomography-magnetic resonance imaging (PET-MRI). A comparable distribution of the therapeutic was observed after administration of a microdose or macrodose. Uptake of the therapeutic by metastatic lymph nodes, lungs, and bone was also demonstrated by PET-MRI with a significantly higher PET signal than in the same organs devoid of metastatic lesions.
Conclusion
Our results demonstrate that PET-MRI following a microdose injection of the agent will accurately reflect the innate biodistribution of the therapeutic. The tools developed in the present study lay the groundwork for the clinical testing of MN-anti-miR10b and other similar therapeutics in patients with cancer.
Individual versus general structured feedback to improve agreement in grant peer review: A randomized controlled trial
Authors: Hesselberg Jan-Ole , Fostervold Knut Inge , Ulleberg Pål , Svege Ida 
Published: May 2021

Abstract
BackgroundVast sums are distributed based on grant peer review, but studies show that interrater reliability is often low. In this study, we tested the effect of receiving a short individual feedback report compared to a short general feedback report on the agreement between reviewers.MethodsA total of 42 reviewers at the Norwegian Foundation Dam were randomly assigned to receive either a general feedback report or an individual feedback report. The general feedback group received one report before the start of the reviews that contained general information about the previous call in which the reviewers participated. In the individual feedback group, the reviewers received two reports, one before the review period (based on the previous call) and one during the period (based on the current call). In the individual feedback group, the reviewers were presented with detailed information on their scoring compared with the review committee as a whole, both before and during the review period. The main outcomes were the proportion of agreement in the eligibility assessment and the average difference in scores between pairs of reviewers assessing the same application.ResultsA total of 2398 paired reviews were included in the analysis. There was no difference between the two groups in terms of the average difference. There was a significant difference between the two groups in the proportion of absolute agreement on whether the application was eligible for the funding programme, with the general feedback group demonstrating a higher rate of agreement. However, the overall levels of agreement remained critically low in both groups and for both outcomes.ConclusionsIndividual feedback did not improve agreement between reviewers. This finding is in line with related studies of journal peer review. The low levels of agreement remain a major concern in grant peer review, and research to identify contributing factors as well as the development and testing of interventions to increase agreement rates are still needed.Trial registrationThe study was preregistered at OSF.io/n4fq3.
In Vitro Anticancer Activity of Five Marine Seaweeds Extract From Egypt Against Human Breast and Colon Cancer Cell Lines
Authors: Mofeed Jelan , Deyab Mohamed , Sabry Abd El-Naser , Ward Fatma 
Published: May 2021

Abstract
Ulva fasciata, U. lactuca, Amphiroa anceps, Corallina mediterranea, and Sargassum filipendula extract were screened for their in vitro anticancer activity against human breast adenocarcinoma cell line (MCF-7) and colorectal carcinoma cell line (HCT-116). Algal extracts exhibited significantly dose-dependent anticancer activity without significant cytotoxicity against normal human fibroblasts. The maximum inhibitory percentages against MCF-7 and HCT-116 cell lines were recorded by U. lactuca (88.5 ± 1.08 %) and A. anceps (86.1 ± 2.88 %) extracts. Algal extracts showed cytotoxic effect against MCF-7 cell line with fifty percent inhibitory concentration (IC50) values ranging from 3.54 ± 1.2 to 21.2 ± 1.1 µg mL-1. The seaweeds extract has a less cytotoxic effect against HCT-116. FTIR and GC-MS analyses of the extracts indicated that the anticancer potential of the tested seaweeds may be returned to the presence of various anticancer compounds such as palmitic acid, oleic acid, retinoic acid, dihydroactinidiolide, thiosemicarbazide, diisobutyl phthalate, and phytol. Thus, seaweed extracts may be a promising natural source of safe anticancer agents against human breast and colon cancers.
Prediction and Compensation Strategy of Contour Error in Multi-Axis Motion System
Authors: Jiang Jiali , Li Bingran , Lin Fuyan , Zhang Hui , Ye Peiqing 
Published: May 2021

Abstract
The contour error of the machined parts is an important index to evaluate the accuracy of CNC machining. Considering multi-axis servo control system, a predictive compensation strategy for contour error is presented in this paper. First, the offline identification method is adopted to establish the transfer function of each motion system. In this case, the relationship between the interpolation command and the feedback position of the grating is determined for the selected machine tool. Thus, before machining, the trajectory information of each motion axis can be predicted according to the interpolation instruction and the transfer relationship. It can be converted into the contour trajectory of the part through kinematic analysis, so as to predict the contour error. Finally, the predicted contour error is compensated into the command trajectory to ensure the contour accuracy of the part to be machined. Compared to existing methods, our method can effectively reduce the trial-produced cycle of parts and avoid unnecessary waste of processing materials. Moreover, without increasing the complexity of the control system and greatly reducing the machining efficiency, the dynamic error caused by the dynamic characteristics of the shaft is reduced. Taking the starfish pattern and the contour line of the impeller as the milling processing experiment case, the contour error after compensation is greatly reduced compared with the contour error processed by the original command. Therefore, the predictive compensation method proposed in this paper can significantly improve the machining accuracy. In addition, it also has application value for trial production of complex curved parts.
DNA Barcode Reference Library Construction and Genetic Diversity and Structure Analysis of Amomum Villosum Lour. Populations in Guangdong Province
Authors: Gong Lu , Zhang Danchun , Ding Xiaoxia , Guan Wan , Qiu Xiaohui , Huang Zhihai 
Published: May 2021

Abstract
Background

Amomum villosum Lour. is the plant of a famous traditional Chinese medicine Amomi Fructus and its habitat has been frequently destroyed that seriously threatened its germplasm resources. The genetic diversity has great significance to the optimization of germplasm resources and protection of populations, but that of A. villosum is unclear. In this study, we analyzed the genetic diversity and genetic structures of A. villosum populations in Guangdong and constructed a local reference DNA barcode library for the purpose of more appropriate protecting measures.
Methods

DNA barcoding and ISSR markers were used to investigate the population genetics of A. villosum. Five universal DNA barcodes were amplified and constructed a DNA barcode reference library. Parameters including percentage of polymorphic sites (PPB), number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity index (H) and Shannon's polymorphism information index (I) were calculated for the assessment of genetic diversity. Genetic structure was revealed by Nei’s gene differentiation coefficient (Gst), total population genetic diversity (Ht), intra-group genetic diversity (Hs) and gene flow (Nm). AMOVA analysis, Mantel tests, UPGMA dendrogram and PCoA analysis were used to elucidate the genetic differentiation and the relationship among populations.
Results

531 sequences of five DNA barcodes were gained and all the sequences of the each barcode had no variation sites. A total of 66 ISSR bands were generated from A. villosum populations by the selected 6 ISSR primers. And 56 bands were amplified polymorphic with the percentage was 84.85% for all the seven A. villosum populations. The A. villosum populations showed high genetic diversity (H = 0.3281, I = 0.4895) whereas the gene flow was weak (Nm = 0.6143). Gst (0.4487) and AMOVA analysis indicated that there’s obvious genetic differentiation among A. villosum populations and more genetic variations existed in within population. The genetic relationship of each population was relatively close as the genetic distances were between 0.0844 and 0.3347.
Conclusions

Overall, this study confirmed a high genetic diversity but weak gene flow of A. villosum in Guangdong province, especially in Amomi Fructus’s Daodi producing area, Yangchun city. And a local DNA barcode reference library containing 531 sequences were subsequently constructed; facilitating the identification, conservation, breeding and cultivation of A. villosum.