bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4–pRB Axis in Fibroblasts at the Invasive Tumor Edge
Authors: Bouchard Gina , Garcia-Marques Fernando Jose , Karacosta Loukia Georgiou , Zhang Weiruo , Bermudez Abel , Riley Nicholas McIlvain , Varma Sushama , Mehl Lindsey Catherine , Benson Jalen Anthony , Shrager Joseph B. , Bertozzi Carolyn Ruth , Pitteri Sharon J. , Giaccia Amato J. , Plevritis Sylvia Katina 
Published: December 2021

Abstract

The invasive leading edge represents a potential gateway for tumor metastasis. The role of fibroblasts from the tumor edge in promoting cancer invasion and metastasis has not been comprehensively elucidated. We hypothesize that cross-talk between tumor and stromal cells within the tumor microenvironment results in activation of key biological pathways depending on their position in the tumor (edge vs. core). Here we highlight phenotypic differences between tumor-adjacent-fibroblasts (TAF) from the invasive edge and tumor core fibroblasts from the tumor core, established from human lung adenocarcinomas. A multiomics approach that includes genomics, proteomics, and O-glycoproteomics was used to characterize cross-talk between TAFs and cancer cells. These analyses showed that O-glycosylation, an essential posttranslational modification resulting from sugar metabolism, alters key biological pathways including the cyclin-dependent kinase 4 (CDK4) and phosphorylated retinoblastoma protein axis in the stroma and indirectly modulates proinvasive features of cancer cells. In summary, the O-glycoproteome represents a new consideration for important biological processes involved in tumor–stroma cross-talk and a potential avenue to improve the anticancer efficacy of CDK4 inhibitors.

Significance:
A multiomics analysis of spatially distinct fibroblasts establishes the importance of the stromal O-glycoproteome in tumor–stroma interactions at the leading edge and provides potential strategies to improve cancer treatment.
See related commentary by De Wever, p. 537
Cancer Needs a Robust “Metadata Supply Chain” to Realize the Promise of Artificial Intelligence
Authors: Chung Caroline , Jaffray David A. 
Published: December 2021

Abstract
Profound advances in computational methods, including artificial intelligence (AI), present the opportunity to use the exponentially growing volume and complexity of available cancer measurements toward data-driven personalized care. While exciting, this opportunity has highlighted the disconnect between the promise of compute and the supply of high-quality data. The current paradigm of ad-hoc aggregation and curation of data needs to be replaced with a “metadata supply chain” that provides robust data in context with known provenance, that is, lineage and comprehensive data governance that will allow the promise of AI technology to be realized to its full potential in clinical practice.
Substrate Characterization and Outcome of Catheter Ablation of Ventricular Tachycardia in Patients With Nonischemic Cardiomyopathy and Isolated Epicardial Scar
Authors: Liuba Ioan , Muser Daniele , Chahal Anwar , Tschabrunn Cory , Santangeli Pasquale , Kuo Ling , Frankel David S. , Callans David J. , Garcia Fermin , Supple Gregory E. , Schaller Robert D. , Dixit Sanjay , Lin David , Nazarian Saman , Kumareswaran Ramanan , Arkles Jeffrey , Riley Michael P. , Hyman Matthew C. , Walsh Katie , Guandalini Gustavo , Arceluz Martin , Pothineni Naga Venkata K. , Zado Erica S. , Marchlinski Francis 
Published: December 2021

Background:
The substrate for ventricular tachycardia (VT) in left ventricular (LV) nonischemic cardiomyopathy may be epicardial. We assessed the prevalence, location, endocardial electrograms, and VT ablation outcomes in LV nonischemic cardiomyopathy with isolated epicardial substrate.

Methods:
Forty-seven of 531 (9%) patients with LV nonischemic cardiomyopathy and VT demonstrated normal endocardial (>1.5 mV)/abnormal epicardial bipolar low-voltage area (LVA, <1.0 mV and signal abnormality). Abnormal endocardial unipolar LVA (≤8.3 mV) and endocardial bipolar split electrograms and predictors of ablation success were assessed.

Results:

Epicardial bipolar LVA (27.3 cm
2
[interquartile range, 15.8–50.0]) localized to basal (40), mid (8), and apical (3) LV with basal inferolateral LV most common (28/47, 60%). Of 44 endocardial maps available, 40 (91%) had endocardial unipolar LVA (24.5 cm
2
[interquartile range, 9.4–68.5]) and 29 (67%) had characteristic normal amplitude endocardial split electrograms opposite the epicardial LVA. At mean of 34 months, the VT-free survival was 55% after one and 72% after multiple procedures. Greater endocardial unipolar LVA than epicardial bipolar LVA (hazard ratio, 10.66 [CI, 2.63–43.12],
P
=0.001) and number of inducible VTs (hazard ratio, 1.96 [CI, 1.27–3.00],
P
=0.002) were associated with VT recurrence.

Conclusions:
In patients with LV nonischemic cardiomyopathy and VT, the substrate may be confined to epicardial and commonly basal inferolateral. LV endocardial unipolar LVA and normal amplitude bipolar split electrograms identify epicardial LVA. Ablation targeting epicardial VT and substrate achieves good long-term VT-free survival. Greater endocardial unipolar than epicardial bipolar LVA and more inducible VTs predict VT recurrence.
A Case of Kinship
Authors: van de Poel Rita , Stolk Sander 
Published: December 2021

Abstract
This article describes onomasiological explorations of Old Frisian and Old English lexis in the semantic field of KINSHIP through a novel, digital approach. In connecting Old Frisian lexis, drawn from the Altfriesisches Handwörterbuch (AFWB), to the overarching structure of A Thesaurus of Old English (TOE), a dataset has been created that shares a semantic framework with the one existing for Old English lexis. The connected resources are shared and analysed using the web application Evoke. Statistical data provided by this tool, such as the degree of lexicalization for this field, facilitates comparative analyses of the two historical languages. As this article demonstrates, the reuse of the onomasiological macrostructure of TOE offers new insights into linguistic and cultural aspects of these two languages and their language communities.
The Causative Stem in Soqotri
Authors: Bulakh M. , Kogan L. , Issa A. , Gumaan I. , Mohammed M. 
Published: December 2021

Abstract
Soqotri is an understudied Semitic language belonging to the Modern South Arabian branch and spoken by the approximately 100,000 inhabitants of the island of Soqotra. The present contribution offers a list of verbs belonging to the so-called causative stem in Soqotri (a cognate of the Arabic stem IV), based on the analysis of the data in the two recently published volumes of the Soqotri oral literature as well as the fieldwork notes of the authors.
Transcatheter closure of multiple paravalvular leaks by a transapical approach under echocardiographic guidance: A case report
Authors: Yi Kang , Ding Fang-Hui , You Tao , Li Hong-Xin , Xu Jian-Guo , Hou Xiao-Dong , Wang Xin-Kuan , Zhang Xin 
Published: December 2021

Case summary A patient who underwent mechanical aortic and mitral valve replacement developed three paravalvular leaks 10 months later. We located the tracks by puncturing the apex cordis under transoesophageal echocardiography guidance alone and puncturing the femoral artery guided by fluoroscopy. Three paravalvular leaks were occluded with a hybridization method simultaneously. The patient was followed up for 24 months and maintained a good condition. Conclusion Multiple paravalvular leaks after double valve replacement can be occluded in patients by the use of different approaches under echocardiographic guidance alone.
From fabric to smart T-shirt: fine tuning an improved robust system to detect arrhythmia
Authors: Malek Abdel Salam , Elnahrawy Ashraf , Anwar Hamed , Naeem Mohamed 
Published: December 2021

Wearable electrocardiogram (ECG) systems should be comfortable, non-stigmatizing, and capable of producing high-quality data. Many different designs of wearable textile ECG systems have recently emerged. Some of them are not considered to be smart garments, whereas most of the others present only the electronic side of the system. Our research work introduces a comprehensive study for an improved single-lead ECG smart shirt to identify automatically premature ventricular contraction as a common form of arrhythmia. For artifact-free results, Marvelous Designer is implemented to design our optimized relaxed slim fit shirt. In addition, a weft-knitted fabric of 80% nylon–20% spandex is used to manufacture the outer part of the shirt. Moreover, lightweight and small size electronic components are integrated to the outer part via low-resistance dry textile electrodes and 100% cotton fabric as an inner layer for easy transmission of weak ECG signals.
Association of endothelial nitric oxide synthase intron 4a/b gene polymorphisms and hypertension: a systematic review and meta-analysis
Authors: Xu Xiru , Ye Woruo , Chen Hanqing , Liu Ming , Jiang Weimin , Fang Zhuyuan 
Published: December 2021

Objective We conducted meta-analysis of relevant case-control trials to determine the association between endothelial nitric oxide synthase (eNOS) intron 4a/b gene polymorphisms and hypertension susceptibility. Methods We searched the PubMed, Cochrane, and Embase databases using relevant keywords and reviewed pertinent literature sources. All articles published up to July 2019 were considered for inclusion. Based on the qualified studies, we performed a meta-analysis of the associations between eNOS intron 4a/b polymorphisms and the risk of hypertension. Results Fourteen studies were included in this meta-analysis, including 3344 cases and 3377 controls. The eNOS intron 4a/b locus was significantly associated with increased susceptibility to hypertension (including essential hypertension) in the overall population, according to dominant, allelic, homozygote, heterozygote, and regressive models, in the mixed population according to the regressive model, and in Caucasians according to the dominant, allelic, heterozygote, and regressive models. The eNOS intron 4a/b locus was also significantly associated with increased susceptibility to essential hypertension in the mixed population according to the heterozygote model. Conclusion eNOS intron 4a/b gene polymorphisms increase susceptibility to hypertension, including essential hypertension.
Effect of sequential eradication therapy on serum osteoprotegerin levels in patients with Helicobacter pylori infection and co-existing inflammatory bowel disease
Authors: Murad Hussam , Rafeeq Misbahuddin , Mosli Mahmoud , Gari Mamdouh , Basheikh Mohammed 
Published: December 2021

Objective To investigate the effect of sequential Helicobacter pylori eradication therapy on serum osteoprotegerin levels in patients with H. pylori infection and co-existing inflammatory bowel disease (IBD). Methods Three groups of patients were involved in this observational cross-sectional study: IBD (n = 83), H. pylori infection (HP, n = 68), and H. pylori infection with co-existing IBD (HP + IBD, n = 52). These groups were compared with a normal control group (NC, n = 50). Serum osteoprotegerin, serum bone alkaline phosphatase (BALP), and fecal calprotectin (FC) levels were measured. Results Serum osteoprotegerin levels were significantly correlated with the simple endoscopic score for Crohn’s disease and Mayo score for ulcerative colitis. The receiver operating characteristic analysis of osteoprotegerin revealed high values for the area under the curve, sensitivity, and specificity. Discriminant analysis illustrated that osteoprotegerin levels significantly differentiated patients with IBD from healthy controls. Osteoprotegerin and FC levels distinguished the IBD and HP + IBD groups from the NC and HP groups. Conclusions Sequential eradication therapy did not affect serum osteoprotegerin levels in patients with H. pylori infection and co-existing IBD. Serum osteoprotegerin elevation might be a marker for IBD development in patients with past or current H. pylori infection.
An exploration of patient satisfaction with and experience of a sexual abuse survivors clinic
Authors: Birdi Gurkiran , Caswell Rachel J , Ross Jonathan DC , Pattison Helen , Ayinde Oluseyi , Lorimer Karen 
Published: December 2021

Background The scale and extent of sexual violence perpetrated in the United Kingdom is being increasingly acknowledged. Support after the initial disclosure is often sought in NHS sexual health clinics. The purpose of this service evaluation was to explore patient satisfaction and experience amongst sexual health clinic attendees who disclosed sexual violence and were subsequently managed in a specialist sexual abuse survivors clinic. Methods Semi-structured interviews were conducted with ten service users and interview transcripts were analysed using interpretative phenomenological analysis (IPA) to assess users’ experiences within the clinic. Results Participants were all female (aged 18–54 years) and had experienced sexual violence between 2 weeks and 15 years prior to interview, and the majority self-identified as White British (6/10). IPA analysis revealed three distinct overarching themes which were important to this group of patients when evaluating their care: delivery of care in the context of judgement and stigma, aspects of care identified as healing or harmful to recovery, and the importance of the processes of providing care. Conclusion Understanding the experiences of sexual violence survivors in healthcare settings can optimise the provision of patient-oriented care and support. This includes ensuring the service user is in control of the consultation, the risks of re-traumatisation are minimised, and individuals receive relevant and accurate information but in a manageable volume and format.
Cost-effectiveness analysis of acupuncture compared with usual care for acute non-specific low back pain: secondary analysis of a randomised controlled trial
Authors: Skonnord Trygve , Fetveit Arne , Skjeie Holgeir , Brekke Mette , Grotle Margreth , Klovning Atle , Aas Eline 
Published: December 2021

Objective: To assess the cost-effectiveness of a single treatment session of acupuncture, when applied in addition to usual care for acute low back pain (ALBP). Methods: Secondary analysis of a multicentre randomised controlled trial in Norwegian general practice. In total, 171 participants with ALBP ⩽14 days were randomised to a control group (CG) receiving usual care or to an acupuncture group (AG) receiving one additional session of Western medical acupuncture alongside usual care. Primary outcome measures for this cost-effectiveness analysis were quality-adjusted life years (QALYs), health care costs and societal costs at days 28 and 365, the incremental cost-effectiveness ratio (ICER) and net monetary benefit (NMB). The NMB was calculated on the basis of the Norwegian cost-effectiveness threshold of NOK 275,000 (USD 35,628) per QALY gained. Missing data were replaced by multiple chained imputation. Results: Eighty-six participants in the CG and 81 in the AG were included in the analysis. We found no QALY gain at day 28. At day 365, the incremental QALY of 0.035 was statistically significant. The differences in health care costs and societal costs were not statistically significant. Three out of four calculations led to negative ICERs (cost saving) and positive NMBs. For the health care perspective at day 365, the ICER was USD –568 per QALY and the NMB was USD 1265, with 95.9% probability of acupuncture being cost-effective. Conclusion: To our knowledge, this is the first cost-effectiveness analysis of acupuncture for ALBP. The findings indicate that acupuncture may be cost-effective from a 1-year perspective, but more studies are needed. Trial registration number: NCT01439412 (ClinicalTrials.gov).
Inter-eye comparison of retinal vascular growth rate and angiographic findings following unilateral bevacizumab treatment
Authors: Bayramoglu Sadik Etka , Sayin Nihat 
Published: December 2021

Purpose To compare retinal vascularization progression rate, final retinal vascularization, and fluorescein angiography (FA) findings in infants who received intravitreal bevacizumab (IVB) treatment in one eye and with spontaneous regression in the other eye. Methods Thirty eyes of 15 infants who underwent IVB in one eye due to asymmetric retinopathy of prematurity, and who had pre-treatment fundus photographs and fluorescein angiography images were included in the study. Horizontal disc diameter (DD), optic disc-to-fovea distance (FD), and the length of temporal retinal vascularization (LTRV) distance were measured by evaluating pre-treatment and FA images. Results The mean ages at the time of treatment and FA were 40.38  ±  3.35 and 68.72  ±  10.52 weeks postmenstrual age, respectively. The pre-treatment LTRV/FD ratio was 3.11  ±  0.41 in the treated eyes and 3.26  ±  0.43 in the non-treated eyes (p  =  0.053). The final LTRV/FD ratio was 4.23  ±  0.38 in the treated group and 4.33  ±  0.37 in the non-treated group (p  =  0.286). Staining of the vessels, hyperfluorescent focus, and irregular branching of the vessels were similar between the groups, respectively (p  =  1.000; p  =  0.250; p  =  0.625). Conclusion The progression rate of retinal vascularization and angiographic findings were similar between the treated eyes and the non-treated eyes. Our study suggests that incomplete retinal vascularization in eyes treated with anti-vascular endothelial growth factor (VEGF) is due to the nature of the disease, and anti-VEGF treatment was not to cause cessation in vascular progression.
IGF-1 is not related to long-term outcome in hyperglycemic acute coronary syndrome patients
Authors: Iswandi Cindya P , van den Berg Victor J , Simsek Suat , Velzen Daan van , Boekel Edwin Ten , Cornel Jan-Hein , Boer Sanneke de , Mulder Maarten de , Akkerhuis K Martijn , Boersma Eric , Umans Victor A , Kardys Isabella 
Published: December 2021

Purpose Insulin-like growth factor-1 (IGF-1) has been associated with both protective and detrimental effects on the development of ischemic heart disease. The relationship between IGF-1 levels and major adverse cardiovascular events (MACE) in acute coronary syndrome (ACS) patients remains unclear. This study aimed to investigate the relationship between IGF-1 admission levels in hyperglycemic ACS patients and: (1) MACE over a 5 years follow-up, (2) type 2 diabetes at discharge, and (3) post-ACS myocardial infarct size and dysfunction. Methods This was a post hoc analysis of the BIOMArCS-2 randomized controlled trial. From July 2008 to February 2012, 276 ACS patients with admission plasma glucose level between 140 and 288 mg/dL were included. Records of the composite of all-cause mortality and recurrent non-fatal myocardial infarction were obtained during 5 years follow-up. Venous blood samples were collected on admission. IGF-1 was measured batchwise after study completion. Oral glucose tolerance test was performed to diagnose type 2 diabetes, whereas infarct size and left ventricular function were assessed by myocardial perfusion scintigraphy (MPS) imaging, 6 weeks post-ACS. Results Cumulative incidence of MACE was 24% at 5 years follow-up. IGF-1 was not independently associated with MACE (HR:1.00 (95%CI:0.99–1.00), p = 0.29). Seventy-eight patients (28%) had type 2 diabetes at discharge, and the highest quartile of IGF-1 levels was associated with the lowest incidence of diabetes (HR:0.40 (95%CI:0.17–0.95), p = 0.037). IGF-1 levels were not associated with post-ACS myocardial infarct size and dysfunction. Conclusions IGF-1 carries potential for predicting type 2 diabetes, rather than long-term cardiovascular outcomes and post-ACS myocardial infarct size and dysfunction, in hyperglycemic ACS patients.
No significant difference in risk of prolonged opioid use following outpatient versus inpatient total shoulder arthroplasty: A propensity matched analysis
Authors: Xiao Michelle , Curtis Daniel M , Cheung Emilie V , Freehill Michael T , Abrams Geoffrey D 
Published: December 2021

Background The purpose of this investigation was to compare rates of filled opioid prescriptions and prolonged opioid use in opioid naïve patients undergoing total shoulder arthroplasty (TSA) in inpatient versus outpatient settings. Methods A retrospective cohort study was conducted using a national insurance claims database. Inpatient and outpatient cohorts were created by identifying continuously enrolled, opioid naïve TSA patients. A greedy nearest-neighbor algorithm was used to match baseline demographic characteristics between cohorts with a 1:1 inpatient to outpatient ratio to compare the primary outcomes of filled opioid prescriptions and prolonged opioid use following surgery between cohorts. Results A total of 11,703 opioid naïve patients (mean age 72.5 ± 8.5 years, 54.5% female, 87.6% inpatient) were included for analysis. After propensity score matching (n = 1447 inpatients; n = 1447 outpatients), outpatient TSA patients were significantly more likely to fill an opioid prescription in the perioperative window compared to inpatients (82.9% versus 71.5%, p < 0.001). No significant differences in prolonged opioid use were detected (5.74% inpatient versus 6.77% outpatient; p = 0.25). Conclusions Outpatient TSA patients were more likely to fill opioid prescriptions compared to inpatient TSA patients. The quantity of opioids prescribed and rates of prolonged opioid use were similar between the cohorts. Level of evidence Therapeutic Level III.
Using environmental DNA methods to improve detectability in an endangered sturgeon (Acipenser sinensis) monitoring program
Authors: Yu Dan , Shen Zhongyuan , Chang Tao , Li Sha , Liu Huanzhang 
Published: December 2021

Abstract
Background
To determine the presence and abundance of an aquatic species in large waterbodies, especially when populations are at low densities, is highly challenging for conservation biologists. Environmental DNA (eDNA) has the potential to offer a noninvasive and cost-effective method to complement traditional population monitoring, however, eDNA has not been extensively applied to study large migratory species. Chinese sturgeon (Acipenser sinensis), is the largest anadromous migratory fish in the Yangtze River, China, and in recent years its population has dramatically declined and spawning has failed, bringing this species to the brink of extinction. In this study, we aim to test the detectability of eDNA methods to determine the presence and relative abundance of reproductive stock of the species and whether eDNA can be used as a tool to reflect behavioral patterns. Chinese sturgeon eDNA was collected from four sites along the spawning ground across an eight month period, to investigate the temporal and spatial distribution using droplet digital PCR (ddPCR).

Results
We designed a pair of specific primers for Chinese sturgeon and demonstrated the high sensitivity of ddPCR to detect and quantify the Chinese sturgeon eDNA concentration with the limit of detection 0.17 copies/μl, with Chinese sturgeon eDNA been intermittently detected at all sampling sites. There was a consistent temporal pattern among four of the sampling sites that could reflect the movement characteristics of the Chinese sturgeon in the spawning ground, but without a spatial pattern. The eDNA concentration declined by approximately 2–3 × between December 2018 and December 2019.

Conclusions
The results prove the efficacy of eDNA for monitoring reproductive stock of the Chinese sturgeon and the e decreased eDNA concentration reflect that Chinese sturgeon may survive with an extremely small number of reproductive stock in the Yangtze River. Accordingly, we suggest future conservation measures should focus on both habitat restoration and matured fish restocking to ensure successful spawning. Overall, this study provides encouraging support for the application of eDNA methods to monitor endangered aquatic species.