bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Methylation-Driven Genes Prognosis Signature and Immune Microenvironment in Epithelial Ovarian Cancer
Authors: Tan Mingzi , Wang Shengtan , Li Feifei , Xu Haoya , Gao Jian , Zhu Liancheng 
Published: October 2021

Abstract
Background

Aberrant gene methylation has been implicated in the development and progression of tumors. We aimed to identity methylation driven genes involved in EOC to establish a prognostic signature for patients with EOC.
Methods

The methylation, RNA expression and clinical data of EOC patients were downloaded from UCSC Xena website. The “MethylMix” R package was employed to identify the methylation driven genes (MDGs), GO and KEGG analyses were performed to identify the biological functions of the identified genes, univariate, LASSO, and multivariate analyses were carried out to reveal key prognostic genes. Combined with clinical parameters, a prognostic risk nomogram model was established, and its predictive value was internally evaluated using AUC curves and externally verified in the GEO database.
Results

111 differentially expressed MDGs were identified, among which six (SLC9A1, MARVELD1, PI3, MFAP4, MSX1, IL18BP) were selected to establish a prognostic risk scoring model. Results of univariate and multivariate analyses indicated that the risk score model was an independent risk factor for EOC. Combined with clinical parameters, a nomogram was constructed, which showed good predictive performance as evidenced by AUC values. The risk scores and the nomogram were externally verified using GSE26193 and GSE53963 datasets. Analysis of mRNA levels of the six genes in OVCAR3 before and after DAC treatment revealed that DAC inhibited the methylation of six genes, thereby increasing mRNA levels of the six genes. GSEA analysis show that associated with the 6 MDGs in the two risk score model groups signaling pathways are closely related to tumor invasion and metastasis. There are significant differences between the two risk score model groups in terms of immune function, check point and TMB.
Conclusions

We identified and verified 6 MDGs that are closely related to the prognosis of ovarian cancer. A prognostic risk score model and nomogram for predicting the prognosis of EOC were constructed based on the six MDGs. It can also effectively reflect the immune environment and immunotherapy response of EOC. These MDGs have great significance to the implementation of individualized treatment and disease monitoring of EOC patients.
The Correlation of EMT-related Genes in the Pathogenesis of Non-Small Cell Lung Cancer and Chronic Obstructive Pulmonary Disease
Authors: Li Lian-Gang , Cai Hangqierang , Zhou Xiao-Bo , Zhang Lan , Jiu Tai , Liu Zhi-Bo , Hui Fang , Tuo Ya-Jun , Jiang Jun 
Published: October 2021

Abstract
Purpose: To analyze the expression of epithelial-mesenchymal transformation (EMT)-related genes in non-small cell lung cancer (NSCLC) and chronic obstructive pulmonary disease (COPD).Methods: Bronchoalveolar lavage fluid samples were collected from patients diagnosed with NSCLC, COPD, and COPD complicated with NSCLC. RNA from cells was extracted with Trizol reagent. Differential genes were screened and validated using the PCR Array and qRT-PCR, respectively.Results: The expressions of ITGB1, VIM, and MMP-9 were higher in the NSCLC group compared to the COPD group (P < 0.05). Moreover, the expressions of ITGB1, VIM, MMP-9, and Notch1 were higher, and the expression of KRT14 was lower in the complicated group compared to the NSCLC group (P<0.05).In the NSCLC subgroup, MMP-9 and Notch1 were highly expressed in the lung adenocarcinoma group (P < 0.05) compared to the squamous cell lung carcinoma group.Conclusions: The upregulation of ITGB1, VIM, MMP-9, and Notch1 and downregulation of KRT14 might be closely related to the occurrence and development of NSCLC, mainly lung adenocarcinoma.
Chemical basis underline the bioactivity fortification of licorice by honey-frying due to the natural deep eutectic solvent characteristics of honey
Authors: Kong Shasha , Li Pengyue , Verpoorte Robert , Wang Jia , Zhu Chao , Dai Yuntao , Chen Shilin 
Published: October 2021

Abstract
Background

Honey has been widely used in medicine plants processing since ancient times. Honey-frying of herbal medicine is a well-known treatment in Chinese medicine. It has been reported that honey-frying can improve the immunomodulatory efficacy of licorice. However, it is still unknown why honey-frying improves the immunomodulatory activity of licorice. And our previous research demonstrated that honey has natural deep eutectic solvent (NADES) characteristics. NADES is a kind of solvent with broad polarity range and has attracted extensive attention of scholars in different fields. In the present study, we investigated chemical basis underline the possible potentiation of honey-frying on licorice to elucidate its chemical mechanism.
Methods

Firstly, immunological experiments were conducted to investigate whether the honey-processing could enhance the immunomodulatory efficacy of licorice. Then, the synergistic mechanism of honey-processed licorice was explored based on cell biological activity test, chemical composition test, bioavailability test, and Fourier transform-infrared (FT-IR) spectra.
Results

Pharmacological experiment verified that honey-processing enhanced the immunomodulatory efficacy of licorice. Moreover, honey increased the total flavonoid and polysaccharide contents in licorice decoction, improved the thermal stability and oral bioavailability of certain pharmacologically active constituents, and augmented their overall immunostimulatory functions. Similar effects of honey were also observed with NADES. The above effects are due to multiple molecular interactions between active compounds and sugar molecules of NADES.
Conclusion

These findings indicate that the biological activities of medicinal plants might be fortified by NADES/honey in the processing of the plant material. At the meantime, these findings provide theoretical and empirical basis for potential novel applications of honey or other NADESs at augmenting the health-promoting effects of medicinal plants.
Association of Plasma Complement System With Brain Structure Deficits in Bipolar and Major Depressive Disorders
Authors: Yu Hua , Ni Peiyan , Tian Yang , Zhao Liansheng , Li Mingli , Li Xiaojing , Wei Wei , Wei Jinxue , Du Xiangdong , Wang Qiang , Guo Wanjun , Deng Wei , Ma Xiaohong , Coid Jeremy , Li Tao 
Published: October 2021

Abstract
Objective Inflammation plays a crucial role in the pathogenesis of major depressive disorder (MDD) and bipolar disorder (BD). However, the underlying neurobiological mechanisms are poorly understood. This study aimed to examine whether the dysregulation of complement components contributes to brain structure deficits in BD and MDD patients. Methods A total of 52 BD patients, 35 MDD patients, and 53 mentally healthy controls were recruited from the inpatient and outpatient departments of West China Hospital of Sichuan University. The human complement panel 2-immunology multiplex assay was used to measure the levels of complement C1q, C3, C3b, C4, factor B, factor H, and properdin. Whole brain-based comparison was performed to investigate differences in gray matter volume and cortical thickness among the BD, MDD, and control groups, and relationships were explored between neuroanatomical differences and levels of complement components.Results The gray matter volume in the medial orbital frontal cortex (mOFC) and middle cingulum decreased in both patient groups, while the cortical thickness of the left precentral and left superior frontal gyrus was affected differently. Log10-transformed concentrations of C1q, C4, factor B, factor H, and properdin were higher in both patient groups than in controls, while levels of C1q, factor H, and properdin showed a significant negative correlation with gray matter volume in the mOFC at the voxel-wise level.Conclusion Greater inflammation in mOFC was observed in BD and MDD patients than in controls. Structural deficits in both patient groups were associated with elevated levels of certain complement factors, providing insight into the neuro-inflammatory pathogenesis of mood disorders.
Comparison of BP26, Omp25 and Omp31 and a Multi-Epitope Based Fusion Protein in Serological Detection of Canine Brucellosis
Authors: Yao Meixue , Liu Mengda , Chen Xia , Li Jianjun , Li Yan , Wei Runyu , Liu Yong , Kang Long , Duan Xiaoxiao , Shao Weixing , Sun Xiangxiang , Fan Xiaoxu , Sun Shufang , Tian Lili , Yin Dehui , Sun Mingjun 
Published: October 2021

Abstract
Background

Brucellosis is one of the most important zoonotic diseases in the world. Canine brucellosis caused mainly by B. canis is seriously neglected and there is lack of accurate diagnostic tools.
Methods

In this study, using 34 brucellosis positive dog sera and 62 negative control sera, the Brucella outer membrane protein of Omp31, BP26, Omp25 and a multi-epitope based fusion protein were evaluated by iELISA for their potential use as antigens in serological diagnosis for canine brucellosis.
Results

The result showed that multi-epitope based fusion protein performed best in distinguishing brucellosis positive and negative dog sera, with positive predictive value (PPV) was 100% and the negative predictive value (NPV) was 98.41%. BP26 and Omp31 showed excellent sensitivity in detecting brucellosis positive dog sera, but their cross reaction to sera infected with Vibrio parahaemolyticus and Listeria monocytogenes may hinder their application as diagnostic reagent. While Omp25 was lack of sufficient sensitivity and just showed limited ability in distinguishing positive and negative dog sera.
Conclusion

The multi-epitope based fusion protein could accelerate the development of diagnostic kit for canine brucellosis currently urgently needed in China.
Household-Specific Physical Activity Levels and Energy Intakes According to the Presence of Metabolic Syndrome in Korean Young Adults: Korean National Health and Nutrition Examination Survey 2016‒2018
Authors: Lee Young-Jun , Park Yeon-Hee , Lee Jung-Woo , Sung Eun-Sook , Lee Hyun-Seob , Park Jonghoon 
Published: October 2021

Abstract
Background: Participation in exercise, and dietary and nutritional intakes have an impact on the risk and prevalence of metabolic syndrome (MetS), but these effects may differ according to whether a person lives alone or in a multi-person household. We analyzed differences in physical activity (PA) levels and energy intake according to household-type and MetS presence among young adults, to investigate the relationships among these factors.Methods: Data of 3,974 young adults (aged > 19 years and < 40 years) were obtained from the Korean National Health and Nutrition Examination Survey (2016‒2018). We analyzed PA levels (occupational and recreational PA, and place movement) and energy intake (total, carbohydrate, protein, and fat). Results: Logistic regression data showed that low PA levels and higher energy intake were associated with MetS incidence and its components in young adults, after adjusting for body mass index, smoking, household-type, and sex. Overall, there was no significant difference in PA level between the MetS and non-MetS group. The total energy intake was higher in the MetS than in the non-MetS group (p < 0.05). These results were similar to those found in multi-person households. In single-person households, the MetS group had significantly lower PA levels (p < 0.01) and total energy intake (p < 0.05) than the non-MetS group.Conclusions: We found significant association among low PA levels, high energy intake, and MetS components in young Korean adults, but with patterns differing according to household type. Energy intake was higher in young adults with than those without MetS, who lived in multi-person households, while young adults with MetS who lived alone had lower PA levels and lower energy intake than those without MetS. These findings highlight the need for different approaches of implementing PA and nutrition strategies according to the type of household in order to prevent MetS.
Use of body composition and phase angle analysis for the assessment of nutritional status and clinical outcomes in critically ill children
Authors: Xiong Zi-Hong , Zheng Xue-Mei , Zhang Guo-Ying , Wu Meng-Jun , Qu Yi 
Published: October 2021

Abstract
BackgroundMalnutrition is highly prevalent in critically ill children in the pediatric intensive care unit .We aimed to investigate the efficiency of bioelectrical impedance analysis (BIA) measurements and phase angle (PhA) analysis for the assessment of nutritional risk and clinical outcomes in critically ill children.MethodsThis single-center observational study included patients admitted to the Pediatric Intensive Care Unit (PICU) of Chengdu Women’s and Children’s Central Hospital. All patients underwent anthropometric measurement in the first 24 h of admission and underwent BIA measurements within 3 days after the admission. The patients were classified into different groups based on body mass index (BMI) for age. Electronic hospital medical records were reviewed to collect clinical data for each patient. All the obtained data were analyzed by the statistics method.ResultsThere were 204 patients enrolled in our study, of which 32.4% were diagnosed with malnutrition. We found that BMI, arm muscle circumference, fat mass, and %body fat were lower in the group with poorer nutritional status (P < 0.05). Evident differences in the score of the Pediatric Risk of Mortality and the duration of mechanical ventilation (MV) among the three groups with different nutritional statuses were observed (P < 0.05). Patients in the severely malnourished group had the longest duration of MV. In the MV groups, there were significant differences (P < 0.05) in albumin level, PhA, and extracellular water/total body water (ECW/TBW ratio). The ECW/TBW ratio and the time for PICU stay had a weak degree of correlation (Pearson correlation coefficient = 0.375). PhA showed a weak degree of correlation with the duration time of medical ventilation (coefficient of correlation = 0.398).ConclusionBIA can be considered an alternative way to assess nutritional status in critically ill children. ECW/TBW ratio and PhA were correlated with PICU stay and duration time of medical ventilation, respectively.
WITHDRAWN: The Molecular Mechanism of ESZM Extract on Treatment ER(-) Breast Cancer in Vivo: Application of “Combat Poison with Poison” Theory
Authors: Ma Liwei , Chen Zhe , Wang Wenbao , Zhang Jingling , Zhang Hongtao , Liu Jicheng 
Published: October 2021

Abstract
Background: Extract of Euphorbia fischeriana S. and Ziziphus jujuba M. (ESZM) is a formula preparation, was composed of Euphorbia fischeriana S. (E. fischeriana) and Ziziphus jujuba M. (Z. jujuba). The aim of the current research was to examine for the first time the pharmacological effects and the underlying molecule mechanism of ESZM extract on the growth inhibition and apoptosis in human ER(-) or ER(+) breast cancer in vivo, and enrich and innovate the theory of traditional Chinese medicine of combating poison with poison. Methods: Growth inhibition, cell cycle arrest and apoptosis in tumor tissues were determined by tumor inhibition rates, cycle analysis kit, Annexin V-FITC/PI staining, Hoechst 33342/PI staining, TUNEL test and TEM observation. Related genes and proteins expression levels were identified by qRT-PCR assay, Western Blotting method, immunohistochemistry and immunofluorescence means. Drug toxicity was analysised by serum biochemistry detection and H&E staining.Results: We found that ESZM extract can inhibite growth, block cell cycle at G2/M phase and induce apoptosis of subcutaneously transplanted tumor, especially ER(-) breast cancer transplanted tumor. Furthermore, we demonstrated that the polyjuice can down-regulate or up-regulate the expression of Bcl-2 family and PI3k/Akt pathway -related signaling molecules proteins and genes in vivo. ESZM extract or E. fischeriana extract increased the levels of ALT, AST, Cr and BUN, and increased liver and kidney toxicity in tumor-bearing mice by serum biochemistry detection and H&E staining.Conclusion: The pharmacological detection suggested that ESZM extract had significant anti-breast cancer effect, especially ER(-) breast cancer xenograft, and this process may be implement through the mitochondrion dependent pathway and the PI3k/Akt signaling pathway. Drug toxicity detection proved that ESZM extract because of compatibility with Z. jujuba was lower toxicity than E. fischeriana extract, was no significant difference in hepatorenal toxicity between ER(-) or ER(+) tumor-bearing mice.
Conventional Coronary Heart Disease Risk Factors in Shahedieh Cohort Population: Yazd, Central Part of Iran
Authors: dehghani Ali , Ashkezari Habib Dehghani , Fallahzadeh Hossein , Soltani Mohammadhosein 
Published: October 2021

Abstract
Background: The prevalence of coronary heart disease (CHD) is increasing worldwide. The main purpose of this study was to investigate the prevalence of CHD and related risk factors in the population of Shahedieh cohort in Yazd, central Iran.Method: This cross-sectional analytical study was performed on the data of the first phase of the Yazd Shahedieh cohort study, which started in 2016 and included about 10,000 people from the urban population of 35-70 years old in Yazd Shahedieh – Yazd, Iran. Descriptive statistics were used to describe the variables and Chi-square test and multiple binary logistic regression models were used for analysis by reporting the modified odds ratio. All analyzes were performed in SPSS 19 software with a significance level of 5%. Results: The prevalence of the CHD was 8.08% (769 patients). The results of multiple binary logistic regression identified aging, low level of education, smoking, female gender, abnormal LDL, family history, diabetes and blood pressure as risk factors for CHD. The odds of getting CHD over the age of 60 was about 6 times than those aged 30-40, in the illiterate people it was about two times than the university. Smoking, family history, hypertension and diabetes increased the odds of getting CHD by 1.67, 1.59, 3.48 and 1.64. Smoking, family history, hypertension and diabetes increased the odds of getting CHD by 1.67, 1.59, 3.48 and 1.64, times than others, respectively.Conclusion: The prevalence of the disease in Iran was relatively high. According to the effect size, the most important risk factors for the disease were age over sixty years and blood pressure.
Evaluating Healthcare Governance Policies: A Scoping Review Protocol
Authors: Finnegan Heather , Halas Gayle , Monnin Caroline , Peckham Allie , Doupe Malcolm 
Published: October 2021

Abstract
Background: Governance policies provide structures and processes through which healthcare systems are managed. Existing literature defines strategies to evaluate operational (e.g. program) and clinical (e.g., patient-provider) healthcare interventions; the equivalent strategies to evaluate governance policies are less well developed. The aim of the proposed scoping review is to examine the extent, nature and range of approaches used to evaluate healthcare governance policies.Methods: Informed by the Joanna Briggs Institute guidelines and the Arksey and O’Malley framework, the proposed study will conduct a keyword search of both health and social sciences databases, including Ageline (EBSCOhost 1978-2020), CINAHL with Full Text (EBSCOhost 1981-2020), EconLIT (EBSCOhost 1886-2020), Medline (Ovid 1946-2020), Global Health (Ovid 1973-2020) and Scopus (1970-2020). The grey literature – Public Documents (desLibris), Theses & Dissertations (ProQuest) and Google Advanced – will also be searched to ensure comprehensive identification of studies. Any evaluation of healthcare governance policies published in English will be included. Findings will be presented using Preferred Reporting Items for Systematic Reviews and Meta-analyses: Extension for Scoping Reviews (PRIMSA-ScR). Our cross-disciplinary team will critically assess the identified literature. Discussion: Findings from the proposed scoping review will provide insight into the ways in which healthcare governance policies have been evaluated and offer future research directions. Based on initial literature scans and consultations with policy workers, we expect to demonstrate the need for more robust (i.e., deliberate, methodical) approaches to evaluate healthcare governance policies, which in turn requires meaningful partnerships to enrich the transactional space between research and policy.
Use of Elamipretide in Patients Assigned Treatment in the Compassionate Use Program: Case Series in Four Rare Orphan Diseases
Authors: Koenig Mary Kay , Russo S. Nick , McBride Kim L. , Bjornsson Hans Tomas , Gunnarsdottir Brynja Bjork , Goldstein Amy , Falk Scott A. 
Published: October 2021

Abstract
Background:Several mitochondrial diseases are caused by pathogenic variants that impair membrane phospholipid remodeling. There are no US Food and Drug Administration (FDA)–approved therapies for these conditions. Elamipretide is a cell-permeable peptide that has been shown to target the inner mitochondrial membrane where it binds to cardiolipin, resulting in improved membrane stability, cellular respiration, and ATP production. Elamipretide has been evaluated in adult and adolescent patients with mitochondrial disorders such as primary mitochondrial myopathy and Barth syndrome, producing clinical and functional improvements. There is little experience with elamipretide in younger patients and those with other mitochondrial diseases. Results:We describe the use of elamipretide in a series of patients with various mitochondrial disorders providing insight regarding dosing of the drug for these patients, especially dosing in young patients. Conclusions:Based on our experience, a dose of approximately 0.5 mg/kg/day is well tolerated in pediatric patients aged < 12 years.
Influence of a special training process on the psychomotor skills of cadet pilots
Authors: Prokopczyk Adam , Wochyński Zbigniew 
Published: October 2021

Abstract
PurposeThe subject of this article is related to the work-activity in the air environment. The aim of the study was to check the effectiveness of the training process based on devices, which using require special biomechanical muscle work. In the research was check the influence of the training process on the Special Aviation Gymnastics Instruments (SAGI) on the improvement of the psychomotor skills, expressed as an increase in the percentage of ability to perform all tasks and the number of reels on a loop.MethodsCadets - second year pilots, studying at the faculty of a pilot. Cadets were carrying out a special pilot training program on SAGI. They were subjected to two exercise tests (reels forward on looping), before and after the period of special training. Exercise tests were performed with the use of a diagnostic and training device used to assess psychomotor skills. During two tests, heart rate (HR) and blood pressure were measured.ResultsThere was a statistically significant increase in the percentage of ability to perform all tasks (p<0.01) and a statistically insignificant increase in the number of reels forward on looping, in test II in relation to test I. In test II, a statistically insignificant higher level of heart rate and blood pressure before and after the effort was noted, compared to test I.ConclusionsIt was found that the training process on SAGI increased the psychomotority level by increasing the percentage of ability to perform all tasks and the number of reels, in test II in relation to test I.