bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early modifications of the gut microbiome in children with hepatic sinusoidal obstruction syndrome after hematopoietic stem cell transplantation
Authors: Masetti Riccardo , Biagi Elena , Zama Daniele , Muratore Edoardo , D’Amico Federica , Leardini Davide , Turroni Silvia , Prete Arcangelo , Brigidi Patrizia , Pession Andrea 
Published: July 2021

AbstractHepatic sinusoidal obstruction syndrome (SOS/VOD) represents a dramatic complication of hematopoietic stem cell transplantation (HSCT), particularly in children. Recent evidence has suggested a role for the gut microbiome (GM) in the context of HSCT and its related complications, but no data are available on the relationship between GM and SOS/VOD. Here, we conducted a retrospective case–control study in allo-HSCT pediatric patients developing or not SOS/VOD and profiled their GM over time, from before the transplant up to 72 days after. A rich and diverse GM before HSCT was found to be associated with a reduced likelihood of developing SOS/VOD. Furthermore, prior to transplant, patients not developing SOS/VOD showed an enrichment in some typically health-associated commensals, such as Bacteroides, Ruminococcaceae and Lachnospiraceae. Their levels remained overall higher until post-transplant. This high-diversity configuration resembles that described in other studies for other HSCT-related complications, including graft-versus-host disease, potentially representing a common protective GM feature against HSCT complications.
Renal denervation does not affect hypertension or the renin-angiotensin system in a rodent model of juvenile-onset polycystic kidney disease: clinical implications
Authors: Li Sheran , Hildreth Cara M. , Rahman Ahmed A. , Barton Sean A. , Wyse Benjamin F. , Lim Chai K. , Pilowsky Paul M. , Phillips Jacqueline K. 
Published: July 2021

AbstractWe examined the effect of total and afferent renal denervation (RDN) on hypertension and the renin-angiotensin system (RAS) in a rodent model of juvenile-onset polycystic kidney disease (PKD). Lewis Polycystic Kidney (LPK) and control rats received total, afferent or sham RDN by periaxonal application of phenol, capsaicin or normal saline, respectively, and were monitored for 4-weeks. Afferent RDN did not affect systolic blood pressure (SBP) determined by radiotelemetry in either strain (n = 19) while total RDN significantly reduced SBP in Lewis rats 4-weeks post-denervation (total vs. sham, 122 ± 1 vs. 130 ± 2 mmHg,P = 0.002, n = 25). Plasma and kidney renin content determined by radioimmunoassay were significantly lower in LPK vs. Lewis (plasma: 278.2 ± 6.7 vs. 376.5 ± 11.9 ng Ang I/ml/h; kidney: 260.1 ± 6.3 vs. 753.2 ± 37.9 ng Ang I/mg/h,P < 0.001, n = 26). These parameters were not affected by RDN. Intrarenal mRNA expression levels of renin, angiotensinogen, angiotensin-converting enzyme (ACE)2, and angiotensin II receptor type 1a were significantly lower, whereas ACE1 expression was significantly higher in the LPK vs. Lewis (allP < 0.05, n = 26). This pattern of intrarenal RAS expression was not changed by RDN. In conclusion, RDN does not affect hypertension or the RAS in the LPK model and indicates RDN might not be a suitable antihypertensive strategy for individuals with juvenile-onset PKD.
Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy
Authors: Acharjee Shaona , Gordon Paul M. K. , Lee Benjamin H. , Read Justin , Workentine Matthew L. , Sharkey Keith A. , Pittman Quentin J. 
Published: July 2021

AbstractMicroglia play an important role in the pathogenesis of multiple sclerosis and the mouse model of MS, experimental autoimmune encephalomyelitis (EAE). To more fully understand the role of microglia in EAE we characterized microglial transcriptomes before the onset of motor symptoms (pre-onset) and during symptomatic EAE. We compared the transcriptome in brain, where behavioral changes are initiated, and spinal cord, where damage is revealed as motor and sensory deficits. We used a RiboTag strategy to characterize ribosome-bound mRNA only in microglia without incurring possible transcriptional changes after cell isolation. Brain and spinal cord samples clustered separately at both stages of EAE, indicating regional heterogeneity. Differences in gene expression were observed in the brain and spinal cord of pre-onset and symptomatic animals with most profound effects in the spinal cord of symptomatic animals. Canonical pathway analysis revealed changes in neuroinflammatory pathways, immune functions and enhanced cell division in both pre-onset and symptomatic brain and spinal cord. We also observed a continuum of many pathways at pre-onset stage that continue into the symptomatic stage of EAE. Our results provide additional evidence of regional and temporal heterogeneity in microglial gene expression patterns that may help in understanding mechanisms underlying various symptomology in MS.
Spreading predictability in complex networks
Authors: Zhao Na , Wang Jian , Yu Yong , Zhao Jun-Yan , Chen Duan-Bing 
Published: July 2021

AbstractMany state-of-the-art researches focus on predicting infection scale or threshold in infectious diseases or rumor and give the vaccination strategies correspondingly. In these works, most of them assume that the infection probability and initially infected individuals are known at the very beginning. Generally, infectious diseases or rumor has been spreading for some time when it is noticed. How to predict which individuals will be infected in the future only by knowing the current snapshot becomes a key issue in infectious diseases or rumor control. In this report, a prediction model based on snapshot is presented to predict the potentially infected individuals in the future, not just the macro scale of infection. Experimental results on synthetic and real networks demonstrate that the infected individuals predicted by the model have good consistency with the actual infected ones based on simulations.
Differences in clinicopathological characteristics, treatment, and survival outcomes between older and younger breast cancer patients
Authors: Abdel-Razeq Hikmat , Iweir Sereen , Abdel-Razeq Rashid , Rahman Fadwa Abdel , Almasri Hanan , Bater Rayan , Taqash Ayat , Abdelkhaleq Hadeel 
Published: July 2021

AbstractIn developing countries, breast cancer is diagnosed at a much younger age. In this study we investigate the dichotomies between older and young breast cancer patients in our region. The study involved two cohorts; older patients (≥ 65 years, n = 553) and younger ones (≤ 40 years, n = 417). Statistical models were used to investigate the associations between age groups, clinical characteristics and treatment outcomes. Compared to younger patients, older patients were more likely to present with advanced-stage disease (20.6% vs. 15.1%, p = .028). However, among those with non-metastatic disease, younger patients tended to have more aggressive pathological features, including positive axillary lymph nodes (73.2% vs. 55.6%, p < .001), T-3/4 (28.2% vs. 13.8%, p < .001) and HER2-positive disease (29.3% vs. 16.3%, p < .001). The 5-year overall survival (OS) rate was significantly better for the younger (72.1%) compared to the older (67.6%), p = .035. However, no significant difference was observed in disease-free survival (DFS) between the two groups.In conclusion, younger patients with breast cancer present with worse clinical and pathological features, albeit a better OS rate. The difference in DFS between the two groups was not insignificant, suggesting that older women were more likely to die from non-cancer related causes.
Room-temperature deformation of single crystals of ZrB2 and TiB2 with the hexagonal AlB2 structure investigated by micropillar compression
Authors: Chen Zhenghao , Paul Bhaskar , Majumdar Sanjib , Okamoto Norihiko L. , Kishida Kyosuke , Inui Haruyuki , Otani Shigeki 
Published: July 2021

AbstractThe plastic deformation behavior of single crystals of two transition-metal diborides, ZrB2 and TiB2 with the AlB2 structure has been investigated at room temperature as a function of crystal orientation and specimen size by micropillar compression tests. Although plastic flow is not observed at all for their bulk single crystals at room temperature, plastic flow is successfully observed at room temperature by the operation of slip on {1$${\bar{1}}$$

1

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00}<11$${\bar{2}}$$

2

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3> in ZrB2 and by the operation of slip on {1$${\bar{1}}$$

1

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00}<0001> and {1$${\bar{1}}$$

1

¯

00}<11$${\bar{2}}$$

2

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0> in TiB2. Critical resolve shear stress values at room temperature are very high, exceeding 1 GPa for all observed slip systems; 3.01 GPa for {1$${\bar{1}}$$

1

¯

00}<11$${\bar{2}}$$

2

¯

3> slip in ZrB2 and 1.72 GPa and 5.17 GPa, respectively for {1$${\bar{1}}$$

1

¯

00}<0001> and {1$${\bar{1}}$$

1

¯

00}<11$${\bar{2}}$$

2

¯

0> slip in TiB2. The identified operative slip systems and their CRSS values are discussed in comparison with those identified in the corresponding bulk single crystals at high temperatures and those inferred from micro-hardness anisotropy in the early studies.
Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition
Authors: Rajametov Sherzod Nigmatullayevich , Yang Eun Young , Cho Myeong Cheoul , Chae Soo Young , Jeong Hyo Bong , Chae Won Byoung 
Published: July 2021

AbstractUnderstanding the mechanism for heat tolerance is important for the hot pepper breeding program to develop heat-tolerant cultivars in changing climate. This study was conducted to investigate physiological and biochemical parameters related to heat tolerance and to determine leaf heat damage levels critical for selecting heat-tolerant genotypes. Seedlings of two commercial cultivars, heat-tolerant ‘NW Bigarim’ (NB) and susceptible ‘Chyung Yang’ (CY), were grown in 42 °C for ten days. Photosynthesis, electrolyte conductivity, proline content were measured among seedlings during heat treatment. Photosynthetic rate was significantly reduced in ‘CY’ but not in ‘NB’ seedlings in 42 °C. Stomatal conductivity and transpiration rate was significantly higher in ‘NB’ than ‘CY’. Proline content was also significantly higher in ‘NB’. After heat treatment, leaf heat damages were determined as 0, 25, 50 and 75% and plants with different leaf heat damages were moved to a glasshouse (30–32/22–24 °C in day/night). The growth and developmental parameters were investigated until 70 days. ‘NB’ was significantly affected by leaf heat damages only in fruit yield while ‘CY’ was in fruit set, number and yield. ‘NB’ showed fast recovery after heat stress compared to ‘CY’. These results suggest that constant photosynthetic rate via increased transpiration rate as well as high proline content in heat stress condition confer faster recovery from heat damage of heat-tolerant cultivars in seedlings stages.
Factors associated with achieving intraocular pressure lower than 15 mmHg by Trabectome surgery in primary open-angle glaucoma
Authors: Nakamura Kentaro , Honda Rio , Soeda Shoichi , Nagai Norihiro , Takahashi Osamu , Kadonosono Kazuaki , Ozawa Yoko 
Published: July 2021

AbstractTo assess good prognostic factors of Trabectome surgery in primary open-angle glaucoma (POAG), clinical records of patients with POAG who underwent Trabectome surgery with/without cataract surgery as the first additive therapy to eye drops between January 2015 and March 2018 were retrospectively reviewed. Overall, data of 79 eyes (79 patients; 50 men; mean age, 68.0 years) up to postoperative 24 months were analyzed. Their mean intraocular pressure (IOP) was 20.4 ± 6.0 mmHg at baseline. Forty-two eyes (53.2%) achieved an IOP < 15 mmHg and ≥ 20% reduction from baseline without additional treatments. Phakic eyes had a better survival probability than pseudophakic eyes after adjusting for age, sex, baseline IOP, best-corrected visual acuity, and eye drop score (hazard ratio 3.096; 95% confidence interval [95% CI] 1.367–7.013; P = 0.007). Phakic eyes treated with combined Trabectome and cataract surgeries (mean survival time, 22.250 months; 95% CI 17.606–26.894) had a better survival probability than pseudophakic eyes treated with Trabectome surgery only (mean survival time, 12.111 months; 95% CI 8.716–15.506; P = 0.009) after the adjustment. Among the eyes treated with Trabectome surgery only, phakic eyes required significantly less additional treatments than pseudophakic eyes (P = 0.04). Trabectome surgery may be indicated for phakic eyes with POAG in addition to eye-drop therapy.
National trends in the prevalence of glycemic control among patients with type 2 diabetes receiving continuous care in Thailand from 2011 to 2018
Authors: Sakboonyarat Boonsub , Pima Warabhorn , Chokbumrungsuk Channarong , Pimpak Taksin , Khunsri Sirikorn , Ukritchon Supak , Imjaijitt Worarachanee , Mungthin Mathirut , Kaewput Wisit , Bhopdhornangkul Bhophkrit , Sathavarodom Nattapol , Tatsanavivat Pyatat , Rangsin Ram 
Published: July 2021

AbstractDiabetes is one of the largest global health problems and exhibits a constantly increasing trend. A series of nationwide hospital-based cross-sectional surveys of clinical outcomes was performed annually from 2011 to 2015 and 2018 among patients with type 2 diabetes aged ≥ 20 years receiving medical care for at least 12 months. A two-stage stratified cluster that was proportional to the size sampling technique was used to select a nationally and provincially representative sample of patients with type 2 diabetes in Thailand. A total of 186,010 patients with type 2 diabetes were enrolled in the study from 2011 to 2018. The prevalence of adequate glycemic control (hemoglobinA1c level < 7.0%) among patients with type 2 diabetes were estimated to be 34.5% (95%CI 33.8–35.2%) in 2011, 33.0% (95%CI 32.4–33.6%) in 2012, 34.7% (95%CI 34.1–35.4%) in 2013, 35.5 (95%CI 34.9–36.1%) in 2014, 35.6 (95%CI 35.0–36.2%) in 2015, and 35.6% (95%CI 35.0–36.2%) in 2018, respectively (p for trend < 0.001). Independent factors related to poor glycemic control (hemoglobinA1c ≥ 7%) were being female, younger aged, living in the northeastern region, received care form hospitals lower than regional level, under universal health coverage scheme, greater duration of diabetes, higher body mass index level and absence of hypertension comorbidity.
Mitofusion is required for MOTS‐c induced GLUT4 translocation
Authors: Bhullar Khushwant S. , Shang Nan , Kerek Evan , Wu Kaiyu , Wu Jianping 
Published: July 2021

AbstractMOTS‐c (mitochondrial ORF of the twelve S-c) is a 16-amino-acid mitochondrial peptide that has been shown to counter insulin resistance and alleviate obesity in vivo. However, the mechanisms involved in the pharmacological action of MOTS-c remain elusive. Based on the ability of MOTS-c to improve insulin resistance and promote cold adaptation, we hypothesized that MOTS-c might play a role in boosting the number of mitochondria in a cell. We found that treatment of mammalian cells with MOTS‐c increased protein levels of TFAM, COX4, and NRF1, which are markers for mitochondrial biogenesis. However, flow cytometry analysis using MitoTracker Green revealed a sharp reduction in the mitochondrial count after MOTS‐c treatment. We then anticipated possible synchronized activation of mitofusion/mitochondrial fusion by MOTS‐c following the onset of mitochondrial biogenesis. This was confirmed after a significant increase in protein levels two GTPases, OPA1, and MFN2, both vital for the fusion of mammalian mitochondria. Finally, we found that inhibition of the two GTPases by TNFα abrogated the ability of MOTS‐c to prompt GLUT4 translocation and glucose uptake. Similar results were obtained by siRNA KD of MFN2 as well. Our results reveal for the first time a pathway that links mitofusion to MOTS-c-induced GLUT4 translocation.
Climate change and tree growth in the Khakass-Minusinsk Depression (South Siberia) impacted by large water reservoirs
Authors: Zhirnova D. F. , Belokopytova L. V. , Meko D. M. , Babushkina E. A. , Vaganov E. A. 
Published: July 2021

AbstractRegional and local climate change depends on continentality, orography, and human activities. In particular, local climate modification by water reservoirs can reach far from shore and downstream. Among the possible ecological consequences are shifts in plant performance. Tree-ring width of affected trees can potentially be used as proxies for reservoir impact. Correlation analysis and t-tests were applied to climatic data and tree-ring chronologies of Pinus sylvestris L. and Larix sibirica Ledeb. from moisture-deficit habitats in the intermontane Khakass-Minusinsk Depression, to assess modification of climate and tree growth by the Krasnoyarsk and Sayano-Shushenskoe Reservoirs on the Yenisei River. Abrupt significant cooling in May–August and warming in September-March occurred after the launch of the turbines in dams, more pronounced near the Sayano-Shushenskoe dam (up to – 0.5 °C in summer and to + 3.5 °C in winter) than near the Krasnoyarsk Reservoir headwaters (– 0.3 °C and + 1.4 °C). Significant lengthening of the warm season was also found for temperature thresholds 0–8 °C. Shifts of seasonality and intensity occurred in climatic responses of all tree-ring chronologies after development of water reservoirs. Patterns of these shifts, however, depended on species-specific sensitivity to climatic modification, distance from reservoirs, and physiographic regions. Mitigation of climate continentality and extremes by reservoirs appears to have offset possible negative effects of warming on tree growth.
Correlation between electrical characteristics and biomarkers in breast cancer cells
Authors: Wang Yang , Li Ying , Huang Jie , Zhang Yan , Ma Ren , Zhang Shunqi , Yin Tao , Liu Shangmei , Song Yan , Liu Zhipeng 
Published: July 2021

AbstractBoth electrical properties and biomarkers of biological tissues can be used to distinguish between normal and diseased tissues, and the correlations between them are critical for clinical applications of conductivity (σ) and permittivity (ε); however, these correlations remain unknown. This study aimed to investigate potential correlations between electrical characteristics and biomarkers of breast cancer cells (BCC). Changes in σ and ε of different components in suspensions of normal cells and BCC were analyzed in the range of 200 kHz–5 MHz. Pearson's correlation coefficient heatmap was used to investigate the correlation between σ and ε of the cell suspensions at different stages and biomarkers of cell growth and microenvironment. σ and ε of the cell suspensions closely resembled those of tissues. Further, the correlations between Na+/H+ exchanger 1 and ε and σ of cell suspensions were extremely significant among all biomarkers (pε < 0.001; pσ < 0.001). There were significant positive correlations between cell proliferation biomarkers and ε and σ of cell suspensions (pε/σ < 0.05). The microenvironment may be crucial in the testing of cellular electrical properties. ε and σ are potential parameters to characterize the development of breast cancer.
Year-round high abundances of the world’s smallest marine vertebrate (Schindleria) in the Red Sea and worldwide associations with lunar phases
Authors: Robitzch Vanessa , Molina-Valdivia Victor , Solano-Iguaran Jaiber J. , Landaeta Mauricio F. , Berumen Michael L. 
Published: July 2021

AbstractVery little is known about the ecology and biology of the smallest marine vertebrates, fishes in the genus Schindleria. Even though over half of named Schindleria species have been identified in the Red Sea, the collection of only very few specimens has been documented. Here, we assessed abundance patterns of nearly two thousand Red Sea long dorsal fin (LDF) adults and found evidence for putative seasonal and spatial differences, likely related to differing habitat and environmental conditions. The highest abundances were outside local seasonal temperature extremes and decoupled from peaks of coral reef fish recruitment. We also found evidence for global trends in abundances related to lunar cycles using our Red Sea data and that from a recently published large collection of specimens from the DANA Expedition (1928–1930). The abundance of adult LDF Schindleria in relation to lunar phases differed significantly, with most Schindleria caught outside the full moon, and mostly during the new moon in the Red Sea and the 3rd quarter moon in the DANA collection. We further suggest that the abundances of Schindleria at coral reefs may be related to reproductive cycles and that these cycles may be timed with the moon as back-calculations of hatch dates from otoliths from the Red Sea significantly resulted after the new moon, making Schindleria the fastest-lived coral reef fish with the shortest generation times. Schindleria could be the most numerous coral reef fish in the world, for which we encourage increased research.
Interpretable and parameter optimized ensemble model for knee osteoarthritis assessment using radiographs
Authors: Bany Muhammad Mohammed , Yeasin Mohammed 
Published: July 2021

AbstractKnee osteoarthritis (KOA) is an orthopedic disorder with a substantial impact on mobility and quality of life. An accurate assessment of the KOA levels is imperative in prioritizing meaningful patient care. Quantifying osteoarthritis features such as osteophytes and joint space narrowing (JSN) from low-resolution images (i.e., X-ray images) are mostly subjective. We implement an objective assessment and quantification of KOA to aid practitioners. In particular, we developed an interpretable ensemble of convolutional neural network (CNN) models consisting of three modules. First, we developed a scale-invariant and aspect ratio preserving model to localize Knee joints. Second, we created multiple instances of "hyperparameter optimized" CNN models with diversity and build an ensemble scoring system to assess the severity of KOA according to the Kellgren–Lawrence grading (KL) scale. Third, we provided visual explanations of the predictions by the ensemble model. We tested our models using a collection of 37,996 Knee joints from the Osteoarthritis Initiative (OAI) dataset. Our results show a superior (13–27%) performance improvement compared to the state-of-the-art methods.
Revaluation of bamboo as biomass
Authors: Vanghele Nicoleta Alexandra , Matache Andreea , Stanciu Mariana Mădălina , Mihalache Dumitru Bogdan 
Published: July 2021

Bamboo is a grassy plant that grows rapidly, is also a renewable natural resource and with a high yield. There are many applications and uses of bamboo, which led to its planting around the world. Among the industries in which bamboo has by now experienced extensive exploitation, we can remember the textile, construction, furniture ; even food, pharmaceutical or cosmetic industries.
Biomass is the plant material that is used as a renewable energy resource. This is achieved others by cultivating plants specially used for energy production or by using resistors from small industrial sectors.
The current paper highlights the potential for the recovery of bamboo as biomass, both in terms of the fact that bamboo is a plant that is believed to be an inexhaustible resource, but also for the wide amount of rest from its multiple uses.