bluuebunny/crossref_metadata_2025_split huggingface dataset:
Buccal Fat Pad- Stromal Vascular Fraction as a New Regenerative Source in Treatment of Maxillofacial Bone Defects: A Case Series Study
Authors: Bohlouli Mahboubeh , Bastami Farshid , Nadjmi Nasser , Nokhbatolfoghahei Hanieh , Khojasteh Arash 
Published: May 2021

Abstract
The study aimed to evaluate the biological properties of the buccal fat pad (BPF) derived tissue stromal vascular fractions (tSVF) using a mechanical device (Minceolip®), compared to BFP- cellular SVF (cSVF) in vitro. Also, the efficiency, safety, and feasibility of the BFP-tSVF were assessed in patients with various maxillofacial defects. Isolation of cSVF and-tSVF from BFP was performed through enzymatic and Mechanical devices. They were evaluated in terms of cell counting and viability, expression surface markers, kinetic growth, and tri-lineage differentiation. Ten patients with jaw bone defects received the BFP- tSVF along with an absorbable gelatin sponge in the clinical stage. Effectiveness and safety were measured by computed tomography. The BFP- tSVF showed high cell viability and cell surface markers, specifically CD45, CD31, and CD34. There was no difference in their kinetic growth and multi-lineage differentiation potential between enzymatic and mechanical approaches. Bone regeneration was significantly enhanced in the patients who received BFP- tSVF than control groups in both segmental (33.50%±4.95 vs. 5.00%±7.07, P-value: 0.042) and partial resection (89.33%±2.08 vs. 10.33%±10.02, P-value: 0.000). There were no intervention-related adverse events reported in the patients. These results confirmed that the BFP- tSVF is effective, safe, and feasible for maxillofacial bone defects patients. It will be necessary to evaluate of clinical efficiency of BFP- tSVF for improving bone regeneration in future studies.
Synergistic Inhibitory Effect of Human Umbilical Cord Matrix Mesenchymal Stem Cells-Conditioned Medium and Atorvastatin on MCF7 Cancer Cells Viablity and Migration
Authors: Abolghasemi Reyhaneh , Ebrahimi-barough Somayeh , Ai Jafar 
Published: May 2021

Abstract
Background: Tumor growth and metastasis eventuate from an interaction between cancer cells and the surrounding extracellular matrix. Recent studies demonstrated inhibitory effects of mesenchymal stem cells on breast tumors. Likewise, the emerging interest in statins as anticancer agents is based on their pleiotropic effects. In the present study, we investigated whether atorvastatin and umbilical cord matrix derived mesenchymal stem cells-conditioned medium alone and combined with each other affect the MCF7 cancer cells viability and interactions.Methods: We measured the viability, apoptosis, and necrosis of MCF7 by MTT assay, Annexin-V/PI staining by flow cytometry, and quantitative real-time PCR. Two-dimensional culture and hanging drop aggregation assay illustrated the morphological changes in single cancer cells and spheroid configuration respectively. We traced the MCF7 migration via scratch-wound healing and trans-well assays. Results: The results showed inhibition of cancer cell viability in all treated groups compared with the control group, but the effect of atorvastatin and conditioned medium combination was further than each one alone. Cancer cells shrinkage and chromatin condensation in the inverted light microscopy and fluorescent staining microscopy indicated apoptosis in treated cells. The annexin V/PI analysis especially in the combination-treated group displayed decreasing in DNA synthesis and cell cycle arrest. The mRNA expressions of caspases 3, 8, 9, and Bcl-2 genes were along with extrinsic and intrinsic apoptosis pathways. Conditioned medium disrupts the connections between cancer cells in the three-dimensional spheroids configuration. The migration of treated cells across the wound area and trans-well diminished, particularly in the combination-treated group. Conclusions: For the first time, the synergistic anti-proliferative and anti-motility effect of atorvastatin along with human umbilical cord mesenchymal stem cells-derived conditioned medium on MCF7 breast cancer cells have been proved. These findings point to some new therapeutic strategies with a focus on the crosstalk between breast cancer cells and microenvironment.
Comparative study on the effectiveness of virtual simulation and jaw model for undergraduate periodontal teaching
Authors: Zhang Jie , Xing Jiawei , Zheng Min , Sheng Jie , Zhang Kailiang , Zhang Baoping 
Published: May 2021

Abstract
Background

To compare the effect of virtual simulation and jaw model on the development of pre-clinical periodontal skills in undergraduate students and to improve future pre-clinical training strategies.
Methods

Sixty volunteer sophomores and juniors from the stomatology at Lanzhou University were enrolled in the current study and randomly divided into four groups: Jaw model (Group J, the control group), Virtual reality (Group V), Virtual-Jaw (Group V-J), and Jaw-Virtual (Group J-V). All of the participants received training on uniform basic periodontal knowledge before completing the first theoretical assessment. Then, they obtained total 8 hours of operation training and completed a second theoretical assessment. Their performance was evaluated using the supragingival scaling processes, and clinical operation scores were graded by a blinded professional using an established standard scoring system.
Results

There was no significant difference in the first theoretical outcomes between the four groups (P > 0.05). The second theoretical scores of the V-J and J-V group (60.00 ± 4.47, 58.33 ± 4.35) were significantly improved as compared with the first theoretical scores (49.67 ± 4.81, 48.00 ± 4.93, P < 0.05). The operation process scores of students in Group V-J and J-V (72.00 ± 5.92; 70.00 ± 3.05) were higher than those in the other two groups (V: 61.67 ± 7.85; J: 60.67 ± 2.58). Additionally, the scaling process performance of students in Group V-J and J-V (53.00 ± 3.05; 63.40 ± 4.39) was improved as compared with that of students in the other two groups (V: 41.90 ± 5.23; J: 47.40 ± 4.31).
Conclusion

The combination of virtual reality and jaw model during periodontal pre-clinical training may improve students’ grades and help them develop professional skill. More importantly, we suggest that the jaw model should be applied prior to virtual reality.
Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam
Authors: Bordoloi Dibosh , Sarma Debojit , Barua Nagendra Sarma , Das Bikram Kishore 
Published: May 2021

Abstract
We carried out the morphological, biochemical and molecular characterization of 20 indigenous Joha (aromatic) rice cultivars of Assam. Distinctiveness, Uniformity and Stability (DUS) characterization of the cultivars revealed polymorphism in thirty-seven traits, establishing distinctiveness for their utilization in breeding programmes. Unweighted Neighbour Joining (UNJ) clustering based on usual Euclidean distances for the polymorphic markers grouped the cultivars into three multi-genotypic clusters. The Joha rice cultivars showed highly significant differences for all the quantitative traits except for panicle length. The genotypic and phenotypic coefficients of variability (GCV & PCV) were high for grain yield ha− 1 (24.62 & 24.85%) and filled grains panicle− 1 (23.69 & 25.02%). All the traits except days to flowering and maturity, flag leaf breadth and spikelet fertility exhibited high heritability along with high to moderate genetic advance, indicating the predominant role of additive gene action. Mahalanobis D2 analysis revealed three multi-genotypic and four mono-genotypic clusters of the cultivars. The cultivars' average polyunsaturated fatty acids were 37.9% oleic acid, 39.22% linoleic acid and 0.5% linolenic acid. The fatty acid profile of Local Joha was superior to the other cultivars as it showed a high level of linoleic and linolenic acid and low saturated fatty acid content. Kon Joha 4 and Ronga Joha contained the highest iron (82.88 mg kg− 1) and zinc (47.39 mg kg− 1), respectively, while protein content of Kon Joha-1 and amylose content of Harinarayan were the highest. Joha-Bihpuria showed the highest gel consistency of 140.50 mm. Kalijeera, Kunkuni Joha, Kon Joha-5, Manimuni Joha and Kon Joha-2 accorded a strong aroma. PCR amplified 174 alleles with a mean value of 2.64 across the 66 polymorphic SSR markers. PIC values ranged from 0.091 to 0.698, with an average of 0.326. The highly informative (PIC > 0.50) markers were RM316, RM283, RM585, RM1388, RM3562, RM171, R1M30, RM118, RM11and RM29 for identification of the twenty aromatic rice cultivars. The UNJ clustering based on Jaccard's coefficients classified the 20 cultivars into three distinct clusters with eight, ten and two entries.
IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells
Authors: Hu Yan , Wang Bing , Yi Ke , Lei Qingjun , Wang Guanghui , Xu Xiaohui 
Published: May 2021

Abstract
Background: Interferon regulatory factor-1 (IRF1) affects the proliferation of colorectal cancer (CRC). Recombinant interferon inducible protein 35 (IFI35) participates in immune regulation and cell proliferation. The aim of the study was to examine whether IRF1 affects the radiation sensitivity of CRC by regulating IFI35.Methods: CCL244 and SW480 cells were divided into five groups: blank control, IFI35 upregulation, IFI35 upregulation control, IFI35 downregulation, and IFI35 downregulation control. All groups were treated with X-rays (6 Gy). IFI35 activation by IRF1 was detected by luciferase reporter assay. The GEPIA database was used to examine IRF1 and IFI35 in CRC. The cells were characterized using CCK-8, EdU, cell cycle, clone formation, flow cytometry, reactive oxygen species (ROS), and mitochondrial membrane potential. Nude mouse animal models were used to detect the effect of IFI35 on CRC.Results: IRF1 can bind to the IFI35 promoter and promote the expression of IFI35. The expression consistency of IRF1 and IFI35 in CRC, according to GEPIA (R = 0.68, p < 0.0001). After irradiation, the upregulation of IFI35 inhibited cell proliferation and colony formation and promoted apoptosis and ROS, while IFI35 downregulation promoted proliferation and colony formation and reduced apoptosis, ROS, and mitochondrial membrane potential were also reduced. The in vivo experiments supported the in vitro ones, with smaller tumors and fewer liver metastases with IFI35 upregulation.Conclusions: IRF1 can promote IFI35 expression in CRC cells. IFI35 is involved in the regulation of radiosensitivity of CRC cells and might be a target for CRC radiosensitization.
In-Silico Studies on Selected Active Constituents of Corbichonia decumbens Against Allergic Bronchopulmonary Aspergillosis
Authors: S Sreeram , A Arunprasath , P Revathi 
Published: May 2021

Abstract
Herbs have been employed in the treatment of various diseases and other medicinal purposes for a long time and they’ve been the zest of our life. Corbichonia decumbens an erect, short-lived succulent herb is found to possess zenith medicinal properties. At a first, the in-silico activity of the plant has been determined to carry out a modification in the Surfactant Protein-D (SP-D) which plays a pivotal role in defense against the Allergic Bronchopulmonary Aspergillosis (ABPA). The SP-D protein has been modified using in-silico tools thus making it more robust in containing ABPA. The protein SP-D when combined with the plant compound 1-methyl-3,5-dinitro-1,2,4-triazole had binding energy of -6.98 and interactions with active site residue ASN-236, LYS-246, GLU-232, PHE-234 and, GLN-236. Being one of the first in-silico works carried out in this plant species it aims to provide a substantial base for future works and unwrap the potential of this herb in all aspects of medicinal plant science.
Selected ‘Starter Kit’ energy system modelling data for Sudan (#CCG)
Authors: Cannone Carla , Allington Lucy , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Brown Edward , Howells Mark , Walker Miriam Zachau , Ahsan Aniq , Charbonnier Flora , Halloran Claire , Hirmer Stephanie , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Wogan David , Barcelona Edito , Rogner Holger , Cronin Jen 
Published: May 2021

Abstract
Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to starting energy system modelling in developing countries, thereby causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Sudan, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020-2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
Selected ‘Starter Kit’ energy system modelling data for Senegal (#CCG)
Authors: Allington Lucy , Cannone Carla , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Brown Edward , Howells Mark , Walker Miriam Zachau , Ahsan Aniq , Charbonnier Flora , Halloran Claire , Hirmer Stephanie , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Wogan David , Barcelona Edito , Rogner Holger 
Published: May 2021

Abstract
Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to starting energy system modelling in developing countries, thereby causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Senegal, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020-2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
Selected ‘Starter Kit’ energy system modelling data for Tunisia (#CCG)
Authors: Cannone Carla , Allington Lucy , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Brown Edward , Howells Mark , Walker Miriam Zachau , Ahsan Aniq , Charbonnier Flora , Halloran Claire , Hirmer Stephanie , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Niet Taco , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Wogan David , Barcelona Edito , Rogner Holger 
Published: May 2021

Abstract
Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to starting energy system modelling in developing countries, thereby causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Tunisia, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020–2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
Spread of Carbapenem-Resistant Enterobacteriaceae in a Region, China: How to Control?
Authors: Wang Jiansheng , Yin Changfu , Yang Weiwei , Lv Yuanpeng , Zhao Peng 
Published: May 2021

Abstract
Background: The increasing number of carbapenem-resistant Enterobacteriaceae (CRE) has become a serious problem globally. This study aimed to elucidate their geographically epidemiological characteristics and explore evidence-based infection control measures.Methods: Carbapenem-resistant genes were identified by polymerase chain reaction (PCR) and sequencing. Bacterial genotyping was studied using multilocus sequence typing (MLST) and wzi typing. The transferability of carbapenemase genes was determined by a broth mating method. The relationships between the rates of antimicrobial consumption and the prevalence of CRE were performed by Pearson's or Spearman's correlation analyses. The elucidation of transmission and the evaluation of control measures involved in electronic medical record review, environmental cultures, and outbreak evolution. Results: A total of 930 phenotypically confirmed CRE isolates collected from 19 hospitals were genotypically characterised. K.pneumoniae (KP) and E.coli isolates were 787 (85.17%) and 96 (10.39%) among 924 carbapenemase-producing Enterobacteriaceae (CPE) isolates. Two major carbapenemase genes KPC-2 and NDM in CPE isolates accounted for 84.63% (n = 782) and 13.74% (n = 127). ST11 comprised 86.32% (631/731) of KPC-2 KP isolates. Wzi typing could discriminate ST11 KP clones and precisely track their transmission. Conjugation assays demonstrated that Some KPC-2- and NDM-bearing plasmids could be conjugatively transferred. The transferability was influenced by different STs and different wzis. CRE patients were becoming increasingly younger due to nosocomial CRE acquisition. The average length of hospitalization of these patients showed a downward trend mainly due to significant increases in voluntarily discharged rates and mortality rates. The frequent transfers of CRE patients between intra- and inter-hospitals were the main driving factors for the CRE increase. No associations between the rates of antibiotics consumption and CRE prevalence were observed. Evidence-based measures could effectively reduce the prevalence of ST11-wzi209 clone but failed to control the dissemination of ST11-wzi141 KP clone. Conclusions: Continued vigilance for the importations should be maintained. Coordinated regional interventions are urgently needed to reduce CRE threat.
Implementation of a Community-Based Weight Loss Program in a North Carolina Healthcare System
Authors: Ro Sarah , Lackey Angelica , Aymes Shannon , McCauley Jennifer , Davis Teneisha , Wang Raene , Stanley Will , Ratner Shana , Machineni Sriram , Fiscus Lynne 
Published: May 2021

Abstract
Current strategies for obesity management in primary care leave many patients inadequately treated. We aimed to evaluate a comprehensive primary care clinic-based weight management program’s clinical effectiveness in a community practice setting. This is an 18 month long pre-post intervention study. We collected and analyzed anthropometric data on the patients enrolled in the community-based weight loss program. All participants received targeted lifestyle counseling, and 78% received pharmacotherapy. The primary outcomes were percent weight loss post-intervention and proportion of patients who achieved a total body weight loss of 5% or greater. Our program served 550 patients over 1952 visits from March 2019 through October 2020. Patients attended an average of 3.5 visits (SD 2.7). A total of 209 patients received adequate exposure to the program (attended at least four visits) and achieved a mean total body weight loss of 5.7% (SD 5.8%, range − 22.8% − 13.7%). Of these patients, 53.1% lost > 5% of total weight. We demonstrate that a community-based weight management program delivered by obesity medicine-trained, primary care clinicians effectively produces clinically significant weight loss in a community setting. Our approach represents a promising, scalable model for expanding access to obesity treatment for the general population.
Changes in the area of ​​Lake Urmia based on Landsat Image
Authors: Sorkhabi Omid Memarian 
Published: May 2021

Abstract
Lake Urmia is an area with dimensions of 130 by 40 km and is one of the salt lakes in the world. This basin is a prominent example of a closed inland basin. Unfortunately, the water level of Lake Urmia has dropped significantly in recent years and has been widely increased in saline lands around. In this research, the study of changes in the area of ​​Lake Urmia based on Landsat image has been studied. The time series of changing the area of ​​Lake Urmia from 1984 to 2016 has a downward trend with a reduction rate of 727 km2. Of course, after 2014, it has increased by several tens of kilometers.
A fuzzy logic monitoring system (FLMS) based on visible light and EMF effects on experimental animals
Authors: Osman Yassir S. K. , Salim Elsayed I. 
Published: May 2021

Recently, there is a large concern focusing on the effects of electromagnetic radiation on human health hazards. So there has been a progress in medical data analysis using different systems such as artificial intelligent techniques. One of these is a Fuzzy logic technique. In this research a fuzzy logic-based monitoring system is used with interference effects on experimental animals. This system shown an ability to support the recent studies with great results and analysis. The performance of the fuzzy inference system was validated through a real data have been collected through an experiment. Therefore, the study designed to investigate the effect of sub-chronic exposure of electromagnetic field on experimental mice. In this experiment, the mice exposed to EMF (12 h/day) in a EMF exposure machine designed especially for exposure of biological.