bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and production of steviol glycosides: recent research trends and perspectives
Authors: Libik-Konieczny Marta , Capecka Ewa , Tuleja Monika , Konieczny Robert 
Published: April 2021

Abstract
Steviol glycosides (SvGls) are plant secondary metabolites belonging to a class of chemical compounds known as diterpenes. SvGls have been discovered only in a few plant species, including in the leaves of Stevia rebaudiana Bertoni. Over the last few decades, SvGls have been extensively researched for their extraordinary sweetness. As a result, the nutritional and pharmacological benefits of these secondary metabolites have grown increasingly apparent. In the near future, SvGls may become a basic, low-calorie, and potent sweetener in the growing natural foods market, and a natural anti-diabetic remedy, a highly competitive alternative to commercially available synthetic drugs. Commercial cultivation of stevia plants and the technologies of SvGls extraction and purification from plant material have already been introduced in many countries. However, new conventional and biotechnological solutions are still being sought to increase the level of SvGls in plants. Since many aspects related to the biochemistry and metabolism of SvGls in vivo, as well as their relationship to the overall physiology of S. rebaudiana are not yet understood, there is also a great need for in-depth scientific research on this topic. Such research may have positive impact on optimization of the profile and SvGls concentration in plants and thus lead to obtaining desired yield. This research summarizes the latest approaches and developments in SvGls production.

Key points
• Steviol glycosides (SvGls) are found in nature in S. rebaudiana plants.
• They exhibit nutraceutical properties.
• This review provides an insight on different approaches to produce SvGls.
• The areas of research that still need to be explored have been identified.
Photosynthetically stimulated bioerosion in symbiotic sponges: the role of glycerol and oxygen
Authors: Achlatis Michelle , van der Zande Rene M. , Webb Alice E. , de Bakker Didier M. , de Nooijer Lennart J. , de Goeij Jasper M. 
Published: April 2021

AbstractOn coral reefs, some of the most aggressive calcium carbonate eroders are dinoflagellate-hosting sponges of the genus Cliona. Like in other marine taxa, the influence of these symbiotic microorganisms on the metabolism of the host sponge, and thereby on erosion of the surrounding ecosystem, is increasingly acknowledged. Despite elevating pH (and hence carbonate saturation state), dinoflagellate photosynthesis promotes bioerosion by their hosts. This paradox might be solved by a spatial isolation of photosynthesis from carbonate dissolution, but it remains unknown which mechanism connects the dinoflagellates’ photosynthesis with the sponge’s bioerosion. Here, we simulate the outcomes of photosynthesis in two separate ways, namely as production of carbon-rich compounds (in this case glycerol) and as an increase in oxygen content. This allows testing their potential to enhance bioerosion rates of sponge holobionts that were preconditioned under variable photosynthetic regimes. We find that glycerol, a commonly shared photosynthate in marine symbioses, stimulates chemical bioerosion rates in the dark of photosynthetically impaired sponges. Chemical bioerosion was all the more limited by availability of sufficient oxygen, while the combination of added glycerol and oxygen boosted chemical bioerosion rates. We argue that under normal physiological conditions, bioerosion is promoted by both organic carbon and oxygen production, and we provide evidence for the storage of photosynthates for night-time use. We further discuss our findings in the context of the current knowledge of the bioerosion mechanism, which we expand by integrating the effects of carbon-rich compounds and oxygen as drivers for bioerosion by Cliona.
Males as victims of intimate partner violence — results from a clinical-forensic medical examination centre
Authors: Wörmann Xenia , Wilmes Sandra , Seifert Dragana , Anders Sven 
Published: April 2021

AbstractOnly few studies have reported on males as victims of intimate partner violence (IPV) so far. The aim of the present study is to analyse frequency and case characteristics of physical violence against male IPV victims examined in a clinical-forensic medical examination centre for victims of violence in Germany over an 11-year period, contributing to a better understanding of IPV in men. Male victims represented 6.2% of IPV cases (n = 167) with a median age of 40 years. Cases were reported to the police in 78.4% before medicolegal examination. In 60.5% of the cases, the perpetrator was the current partner, and 82% occurred in a domestic environment with a predominance of female offenders. In more than half of the cases (57.5%), the victims consulted the examination centre without prior healthcare utilisation. About one-third of the victims reported previous IPV (31.7%). The findings point to the relevance of men as victims of IPV, case group–specific risk factors, injury-dependent behaviour related to healthcare utilisation, the need to establish or strengthen specialised support services for affected men and underscore the importance of clinical-forensic services in documenting and assessing violence-related injuries.
Ecovillage foodscapes: zooming in and out of sustainable food practices
Authors: Ulug Ciska , Trell Elen-Maarja , Horlings Lummina 
Published: April 2021

AbstractThis article uses foodscapes as a lens to explore the potential of ecovillages’ food practices towards enhancing sustainable food systems. Ecovillages are collective projects where members attempt to integrate sustainability principles into daily community life. In these communities, food acts, not only as an element of social life, but also as a venue through which to interact with mainstream food systems and society. Yet, how food practices at ecovillages contribute to sustainable food systems remains vague. This article proposes foodscapes, as a lens, for exploring the sustainability potential of place-based food practices in ecovillages, while also directing attention to how these practices intersect with networks at broader social and spatial scales. It asks, how can we better understand and draw from sustainable food practices, when considering these as both, place-based and relational? And what is the potential and the role of ecovillage communities to contribute to broader sustainable food system change? Drawing on ethnographic and food mapping methods, the article explores selected food practices at three ecovillage communities in the United States. Using social practice theory for “zooming in” on place-based practices and “zooming out” to examine relational networks, we investigate how these communities create internally sustainable food systems, while externally bridging themselves with broader urban and rural communities. Through viewing ecovillage food practices as place-based and relational, we develop a broader and spatially-focused understanding of food system sustainability.
Whistleblower rewards, false reports, and corporate fraud
Authors: Buccirossi Paolo , Immordino Giovanni , Spagnolo Giancarlo 
Published: April 2021

AbstractIt is often claimed that rewards for whistleblowers lead to fraudulent reports, but for several US programs this has not been a major problem. We model the interaction between rewards for whistleblowers, sanctions against fraudulent reporting, judicial errors, and standards of proof in the court case on a whistleblower’s allegations and the possible follow-up for fraudulent allegations. Balancing whistleblower rewards, sanctions against fraudulent reports, and courts’ standards of proof is essential for these policies to succeed. When the risk of retaliation is severe, larger rewards are needed and so are tougher sanctions against fraudulent reports. The precision of the legal system must be sufficiently high, hence these programs are not viable in weak institution environments, where protection is imperfect and court precision low, or where sanctions against false reporting are mild.
In vivo analysis of a proprietary glass-based adhesive for sternal fixation and stabilization using rabbit and sheep models
Authors: Mehrvar Cina , Deignan Emily , Hurtig Mark , Cohen Gideon , Zalzal Paul , Safir Oleg , Alhalawani Adel , Papini Marcello , Towler Mark R. 
Published: April 2021

AbstractWire cerclage remains the standard method of care for sternal fixation, following median sternotomy, despite being beset with complications. An emerging treatment option has been to augment the wires with an adhesive. A patented ionomeric glass (mole fraction: SiO2:0.48, ZnO:0.36, CaO:0.12, SrO:0.04) has been used to formulate GPC+, a glass polyalkenoate cement (GPC), by mixing it with poly(acrylic) acid (PAA) and de-ionized water. In a human cadaver study, this material, when applied with wire cerclage, was able to significantly reduce sternal instability. However, the material has yet to be tested in pertinent animal models. Here, after a series of physical and mechanical tests to confirm suitability of the experimental material for implantation, three samples of GPC+ were implanted in either the tibia or femur of three different rabbits, alongside sham defects, for two different time modalities. A further seven samples of GPC+ and one poly(methyl methacrylate) control (PMMA) were implanted in either the tibia or femur of two different sheep. The sheep containing the PMMA was sacrificed at 8 weeks and the other at 16 weeks, to evaluate time dependent biological response. Upon sacrifice, microCT images were acquired and histology slides prepared for analysis. All three GPC+ samples implanted in the rabbit model, for the two time modalities, were characterized by minimal bone resorption along with a mild inflammatory response. Five of the seven GPC+ materials implanted in the sheep model (all three implanted for 8 weeks and two of those implanted for 16 weeks) were associated with mild to moderate immune response, comparable to that observed with PMMA, as well as mild bone resorption. The remaining two GPC + materials (implanted in the sheep model for 16 weeks) exhibited no bone resorption or inflammatory response and appeared to stimulate increased bone density at the implant site. These results suggest that GPC + can be a viable bone adhesive for use in hard tissue applications such as sternal fixation and stabilization.
Mobilization Under Threat: Emotional Appeals and Pro-Opposition Political Participation Online
Authors: Young Lauren E. 
Published: April 2021

AbstractLarge numbers of people living under authoritarian governments participate in pro-opposition politics despite sometimes significant risks. Increasing amounts of this political participation are taking place and being organized online. Do emotions play a causal role in inducing pro-opposition participation in authoritarian regimes? Can emotions that mobilize participation be spread via social media? Through an experiment carried out by an opposition party in Zimbabwe, I test whether campaign appeals to opposition supporters’ emotions affect their level of political participation in online pro-opposition discussions. I find that across two different issue areas, randomly assigned anger appeals increase participation on average by 0.4 standard deviations more than enthusiasm appeals with the same informational content. In real terms, this represents between 30% and 170% more participation in the groups assigned to the anger appeals across four different measures of participation. There is little evidence that these effects are stronger in areas with less poverty or that have historically been affected by more violence, or when coupled with messages emphasizing personal power. These results suggest that anger appeals that highlight economic grievances can be an important force for mobilizing online political participation in repressive environments.
1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5′-UTR of SARS-CoV-2
Authors: Vögele Jennifer , Ferner Jan-Peter , Altincekic Nadide , Bains Jasleen Kaur , Ceylan Betül , Fürtig Boris , Grün J. Tassilo , Hengesbach Martin , Hohmann Katharina F. , Hymon Daniel , Knezic Bozana , Löhr Frank , Peter Stephen A. , Pyper Dennis , Qureshi Nusrat S. , Richter Christian , Schlundt Andreas , Schwalbe Harald , Stirnal Elke , Sudakov Alexey , Wacker Anna , Weigand Julia E. , Wirmer-Bartoschek Julia , Wöhnert Jens , Duchardt-Ferner Elke 
Published: April 2021

AbstractThe SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5′-untranslated region (5′-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive 1H, 13C, 15N and 31P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
Mutual learning-based efficient synchronization of neural networks to exchange the neural key
Authors: Sarkar Arindam 
Published: April 2021

AbstractSynchronization of two neural networks through mutual learning is used to exchange the key over a public channel. In the absence of a weight vector from another party, the key challenge with neural synchronization is how to assess the coordination of two communication parties. There is an issue of delay in the current techniques in the synchronization assessment that has an impact on the security and privacy of the neural synchronization. In this paper, to assess the complete coordination of a cluster of neural networks more efficiently and timely, an important strategy for assessing coordination is presented. To approximately determine the degree of synchronization, the frequency of the two networks having the same output in prior iterations is used. The hash is used to determine if both the networks are completely synchronized exactly when a certain threshold is crossed. The improved technique makes absolute coordination between two communication parties using the weight vectors’ has value. In contrast, with existing approaches, two communicating parties who follow the proposed approach will detect complete synchronization sooner. This reduces the effective geometric likelihood. The proposed method, therefore, increases the safety of the protocol for neural key exchange. This proposed technique has been passed through different parametric tests. Simulations of the process show effectiveness in terms of cited results in the paper.
The Alignment of Real-World Evidence and Digital Health: Realising the Opportunity
Authors: Khosla Sajan , Tepie Maurille Feudjo , Nagy Mark J. , Kafatos George , Seewald Michael , Marchese Stephanie , Liwing Johan 
Published: April 2021

AbstractIn the new era of healthcare digitalization, there is a golden opportunity in the overlap between digital health and Real-World Evidence (RWE). In this commentary, we define RWE and digital health and investigate their intersection. We describe the stages in the RWE value chain critical to the evidence generation process, how these stages change with new digital technologies and the opportunities and challenges that arise from how these stages evolve—including their application for stakeholders such as patients, physicians and regulators. We also discuss the current published guidelines and frameworks regarding digital health. We categorise these publications in terms of their clarity as “Extensive”, “Intermediate” or “Basic” and according to whether they encompass all levels of digital health or are more focussed in their guidance. Finally, we provide recommendations to increase synergy between RWE and digital health.
Risk-Based Monitoring in Clinical Trials: Past, Present, and Future
Authors: Barnes Brian , Stansbury Nicole , Brown Debby , Garson Lauren , Gerard Geoff , Piccoli Nickolas , Jendrasek Debra , May Nick , Castillo Vanesa , Adelfio Anina , Ramirez Nycole , McSweeney Andrea , Berlien Ruth , Butler Paula Jo 
Published: April 2021

AbstractRisk-based monitoring (RBM) is a powerful tool for efficiently ensuring patient safety and data integrity in a clinical trial, enhancing overall trial quality. To better understand the state of RBM implementation across the clinical trial industry, the Association of Clinical Research Organizations (ACRO) conducted a landscape survey among its member companies across 6,513 clinical trials ongoing at the end of 2019. Of these trials, 22% included at least 1 of the 5 RBM components: key risk indicators (KRIs), centralized monitoring, off-site/remote-site monitoring, reduced source data verification (SDV), and reduced source document review (SDR). The implementation rates for the individual RBM components ranged 8%–19%, with the most frequently implemented component being centralized monitoring and the least frequently implemented being reduced SDR. When the COVID-19 pandemic emerged in early 2020, additional data were collected to assess its impact on trial monitoring, focusing specifically on trials switching from on-site monitoring to off-site/remote-site monitoring. These mid-pandemic data show that the vast majority of monitoring visits were on-site in February 2020, but an even higher percentage were off-site in April, corresponding with the first peak of the pandemic. Despite this shift, similar numbers of non-COVID-related protocol deviations were detected from February through June, suggesting little or no reduction in monitoring effectiveness. The pre- and mid-pandemic data provide two very different snapshots of RBM implementation, but both support the need to promote adoption of this approach while also highlighting an opportunity to capitalize on the recent shift toward greater RBM uptake in a post-pandemic environment.
THE MCKINSEY–TARSKI THEOREM FOR LOCALLY COMPACT ORDERED SPACES
Authors: BEZHANISHVILI GURAM , BEZHANISHVILI NICK , LUCERO-BRYAN JOEL , VAN MILL JAN 
Published: April 2021

AbstractWe prove that the modal logic of a crowded locally compact generalized ordered space is$\textsf {S4}$. This provides a version of the McKinsey–Tarski theorem for generalized ordered spaces. We then utilize this theorem to axiomatize the modal logic of an arbitrary locally compact generalized ordered space.
Ecohydrological controls on apparent rates of peat carbon accumulation in a boreal bog record from the Hudson Bay Lowlands, northern Ontario, Canada
Authors: Davies Marissa A. , Blewett Jerome , Naafs B. David A. , Finkelstein Sarah A. 
Published: April 2021

AbstractA multiproxy Holocene record from a bog in the Hudson Bay Lowlands, northern Ontario, Canada, was used to evaluate how ecohydrology relates to carbon accumulation. The study site is located at a somewhat higher elevation and on coarser grained deposits than the surrounding peatlands. This promotes better drainage and thus a slower rate of carbon accumulation relative to sites with similar initiation age. The rate of peat vertical accretion was initially low as the site transitioned from a marsh to a rich fen. These lower rates took place during the warmer temperatures of the Holocene thermal maximum, confirming the importance of hydrological controls limiting peat accretion at the local scale. Testate amoebae, pollen, and plant macrofossils indicate a transition to a poor fen and then a bog during the late Holocene, as the carbon accumulation rate and reconstructed water table depth increased. The bacterial membrane lipid biomarker indices used to infer paleotemperature show a summer temperature bias and appear sensitive to changes in peat type. The bacterial membrane lipid biomarker pH proxy indicates a rich to a poor fen and a subsequent fen to bog transition, which are supported by pollen, macrofossil, and testate amoeba records.
Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid
Authors: Bu Xianyong , Wang Xiaodan , Lin Zhideng , Wang Chunling , Li Lingyu , Liu Shubin , Shi Qingchao , Qin Jian G. , Chen Liqiao 
Published: April 2021

AbstractThis study evaluated the effects of dietary myo-inositol (MI) on growth performance, antioxidant status and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. Crabs (4·58 (sem 0·05) g) were fed four diets including a normal lipid diet (N, containing 7 % lipid and 0 mg/kg MI), N with MI supplementation (N + MI, containing 7 % lipid and 1600 mg/kg MI), a high lipid diet (H, containing 13 % lipid and 0 mg/kg MI) and H with MI supplementation (H + MI, containing 13 % lipid and 1600 mg/kg MI) for 8 weeks. The H + MI group showed higher weight gain and specific growth rate than those in the H group. The dietary MI could improve the lipid accumulations in the whole body, hepatopancreas and muscle as a result of feeding on the high dietary lipid (13 %) in crabs. Besides, the crabs fed the H + MI diets increased the activities of antioxidant enzymes but reduced the malondialdehyde content in hepatopancreas compared with those fed the H diets. Moreover, dietary MI enhanced the expression of genes involved in lipid oxidation and exportation, yet reduced lipid absorption and synthesis genes expression in the hepatopancreas of crabs fed the H diet, which might be related to the activation of inositol 1,4,5-trisphosphate receptor (IP3R)/calmodulin-dependent protein kinase kinase-β (CaMKKβ)/adenosine 5’-monophosphate-activated protein kinase (AMPK) signalling pathway. This study demonstrates that MI could increase lipid utilisation and reduce lipid deposition in the hepatopancreas of E. sinensis fed a high lipid diet through IP3R/CaMKKβ/AMPK activation. This work provides new insights into the function of MI in the diet of crustaceans.