bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of thyroglobulin in the lymph nodes recurrence of papillary thyroid carcinoma undergoing total thyroidectomy
Authors: Xing Zhichao , Qiu Yuxuan , Li Zhe , Zhang Lingyun , Fei Yuan , Zhu Jingqiang , Su Anping 
Published: January 2021

Abstract
Background: To investigate the association between postoperative lymph nodes (LNs) recurrence and distinct serum thyroglobulin (Tg) levels in patients with papillary thyroid carcinoma (PTC).Methods: This study included PTC patients who underwent total thyroidectomy (TT) with at least central neck dissection and then re-operated due to recurrence of LNs between January 2013 and June 2018. These patients were grouped by negative or positive serum Tg levels according to the American Thyroid Association guidelines. Results: Of the 60 included patients, 49 underwent radioactive iodine (RAI) treatment. Maximum unstimulated Tg (uTg) ≥ 0.2 ng/mL were associated with larger diameter of recurrent LNs (P = 0.027), and higher rate of metastatic LNs (P < 0.001). Serum-stimulated Tg (off-Tg) ≥ 1ng/mL (P = 0.047) and unstimulated Tg (on-Tg) ≥ 0.2 ng/Ml (P = 0.013) were associated with larger diameter of recurrent LNs. Number of metastatic LNs ≥ 8 was an independent predictor for postoperative maximum uTg ≥ 0.2 ng/mL (OR = 8.767; 95% CI =1.392-55.216; P = 0.021). Ratio of metastatic LNs ≥ 25% was an independent predictor for off-Tg ≥ 1 ng/mL (OR = 20.997; 95% CI =1.649-267.384; P = 0.019). Conclusion: Postoperative Tg-positive status was associated with larger size of recurrent LNs. Number of metastatic LNs ≥8 and ratio of metastatic LNs ≥ 25% were independent predicators for uTg-positive and off-Tg-positive status, respectively.
SARS-CoV-2: Preliminary study of infected human nasopharyngeal tissue by high resolution microscopy.
Authors: Mondeja Brian , Valdes Odalys , Resik Sonia , Vizcaino Ananayla , Acosta Emilio , Montalván Adelmo , Paez Amira , Mune Mayra , Rodríguez Roberto , Valdés Juan , Gonzalez Guelsys , Sanchez Daisy , Falcón Viviana , González Yorexis , Kourí Vivian , Group The IPK Virology Research , Díaz Angelina , Guzmán Maria 
Published: January 2021

Abstract
BackgroundThe novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. Methods Nasopharyngeal swabs were collected from two Cuban individuals, one asymptomatic and RT-PCR negative (negative control) and the other from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by Scanning Electron Microscopy (SEM), Confocal Microscopy (CM) and, Atomic Force Microscopy (AFM). Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. Results The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by scanning electron microscopy (SEM). Viral antigen was identified in the apical cells zone by CM. Conclusions The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use.
Predicting Osteoporosis with Body Compositions in Postmenopausal Women: A Noninvasive Method
Authors: Hsu Wei-Hsiu , Hsu Wei-Bin , Fan Chun-Hao , Hsu Wen-Wei Robert 
Published: January 2021

Abstract
Background: The prevalence of osteoporosis is rising steadily as the aging population increases. Bone mineral density (BMD) assessment is a more effort procedure to analyze osteoporosis. However, the accessibility and radiation exposure limited its role in community screening. A more convenient approach for screening is suggested.Methods: A total of 363 postmenopausal women over 50 age were included in this study and assessed with the body composition [including fat-free mass (FFM), fat mass (FM), and basal metabolic rate (BMR)] and BMD. Normal distributions and correlation coefficients among variables were calculated using the Shapiro-Wilk test and Pearson’s correlation analysis, respectively. A receiver operating characteristic (ROC) curve was plotted and the area under ROC curves (AUC) was determined the optimal cut-off values of the body composition variables for osteoporosis prediction.Results: The correlation coefficient of FFM, FM, FM ratio and BMR with femur neck T-score were 0.373, 0.266, 0.165, and 0.369, respectively while with spine T-score were 0.350, 0.251, 0.166, and 0.352, respectively (p<0.01 for all). FFM, FM, and BMR showed an optimal cut-off value of 37.9 kg, 18.6 kg, and 1187.5 kcal for detecting osteoporosis.Conclusions: The present study provided a model to predict osteoporosis in postmenopausal women and the optimal cut-off value of FFM, FM, and BMR could be calculated in Asian population. Among these factors, BMR seemed a better predictor than others. The BMR could be a target for exercise intervention in postmenopausal women for maintaining or improving BMD.
Transcriptomes analysis reveals novel insight into the molecular mechanisms of somatic embryogenesis in Hevea brasiliensis
Authors: Wang Ying , Li Hui-Liang , Zhou Yong-Kai , Guo Dong , Zhu Jia-Hong , Peng Shiqing 
Published: January 2021

Abstract
Background: Somatic embryogenesis (SE) is a promising technology for plant vegetative propagation, which has an important role in tree breeding. Though rubber tree (Hevea brasiliensis Muell. Arg.) SE has been founded, few late SE-related genes have been identified and the molecular regulation mechanisms of late SE are still not well understood. Results: In this study, the transcriptomes of embryogenic callus (EC), primary embryo (PE), cotyledonary embryo (CE), abnormal embryo (AE), mature cotyledonary embryo (MCE) and withered abnormal embryo (WAE) were analyzed. A total of 887,852,416 clean reads were generated, 85.92% of them were mapped to the rubber tree genome. The de novo assembly generated 36937 unigenes. The differentially expressed genes (DEGs) were identified in the pairwise comparisons of CE vs. AE and MCE vs. WAE, respectively. The specific common DEGs were mainly involved in the phytohormones signaling pathway, biosynthesis of phenylpropanoid and starch and sucrose metabolism. Among them, hormone signal transduction related genes were significantly enriched, especially the auxin signaling factors (AUX-like1, GH3.1, SAUR32-like, IAA9-like, IAA14-like, IAA27-like, IAA28-like and ARF5-like). The transcription factors including WRKY40, WRKY70, MYBS3-like, MYB1R1-like, AIL6 and bHLH93-like were characterized as molecular markers for rubber tree late SE. CML13, CML36, CAM-7, SERK1 and LEAD-29-like were also related to rubber tree late SE. In addition, histone modification had crucial roles during rubber tree late SE. Conclusions: This study provides important information to elucidate the molecular regulation during rubber tree late SE.
Laparoscopic Radical Antegrade Modular Pancreatosplenectomy: Preliminary Experience with 10 Cases
Authors: Zhang Renchao , Gan Xin-Jun , Song Wei , Shi Song-Tao , Yu Hui-Fang , Mou Yi-Ping 
Published: January 2021

Abstract
BackgroundThe radical antegrade modular pancreatosplenectomy (RAMPS) which is a reasonable surgical approach for left-sided pancreatic cancer is emphasis on the complete resection of regional lymph nodes and tumor-free margin resection. Laparoscopic radical antegrade modular pancreatosplenectomy(LRAMPS) has been rarely performed, with only 49 cases indexed on PubMed. In this study, we present our experience of LRAMPS.Methods: From December 2018 to February 2020, 10 patients underwent LRAMPS for pancreatic cancer at our department. The data of the patient demographics, intraoperative variables , postoperative hospital stay, morbidity, mortality, pathologic findings and follow-up were collected.Results：LRAMPS was performed successfully in all the patients. The median operative time was 235 minutes (range, 212-270min), with an EBL of 120ml(range,100-200ml) . Postoperative complications occurred in 5 (50.0%) patients. Three patients developed a grade B pancreatic fistula. There was no postoperative 30-day mortality and reoperation. The median postoperative hospital stay was 14 days(range,9- 24d).The median count of retrieved lymph nodes was 15 (range, 13–21), and four patients (40%) had malignant-positive lymph nodes. All cases achieved a negative tangential margin and R0 resection.Median follow-up time was 11 months (range, 3–14m). Two patients developed disease recurrence(pancreatic bed recurrence and liver metastasis) 9 months,10 months after surgery,respectively. Others survived without tumor recurrence or metastasis．ConclusionsLRAMPS is technically safe and feasible procedure in well-selected patients with pancreatic cancer in the distal pancreas. The oncologically outcomes need to be further validated based on additional large-volume studies.
Comparison of pregnancy outcomes in young patients following fresh versus frozen single blastocyst transfer:A retrospective study
Authors: wu yanhong , Liu Chang , Gong Chaochao , Xi Haitao , Fu Yanghua , Zhao Junzhao 
Published: January 2021

Abstract
Background：Previous studies have shown that, in young women, single blastocyst transfer can achieve satisfactory pregnancy results; however, few studies exist regarding the difference between fresh and frozen-thawed single blastocyst transfer. To provide further clinical strategies for single blastocyst transfer, the purpose of this retrospective analysis was to compare the pregnancy outcomes of young patients who received fresh or frozen-thawed single blastocyst transfer.Methods: A retrospective analysis of patients, aged ≤35 years, who underwent single blastocyst transfer was conducted from January 2018 to December 2018 in the reproductive center of the Second Affiliated Hospital of Wenzhou Medical University (Wenzhou, China). In total, 901 patients were included and were divided into two groups, based on the type of transfer cycle: the fresh embryo transfer cycle group (group A; n=693) and the frozen-thawed embryo transfer cycle group (group B; n=208). The laboratory and pregnancy outcomes were compared between the groups.Results：The number of oocytes retrieved in group B was significantly higher than in group A (P＜0.05). The early miscarriage rate in group B was significantly higher than in group A and was significantly different (P＜0.05). Frozen-thawed single blastocyst transplantation was an independent risk factor for early miscarriage. Other basic conditions and obstetric pregnancy outcomes were not significantly different between the two groups.Conclusions：Favourable pregnancy outcomes could be obtained with fresh and frozen-thawed single blastocyst transfer in young patients. However, because the early miscarriage rate was higher in the frozen-thawed embryo transfer group, further research is needed to determine the cause and possible solutions.
Identification and screening of host proteins interacting with ORFV-ORF047 protein
Authors: Chen Guohua , He Xiaobing , jia Huaijie , Fang Yongxiang , Wang Xiaoxia , Lou Zhongzi , yang Fan , Li Weike , Jing Zhizhong 
Published: January 2021

Abstract
Background：Orf virus (ORFV) is a member of the genus Parapoxvirus and family Poxviridae. The virus has a worldwide distribution and infects sheep, goats, humans, and wild animals. However, due to the complex structure of the poxvirus, the underlying mechanism of the entry and infection by ORFV remains largely unknown. ORFV ORF047 encodes a protein named L1R. Poxviral L1R serves as the receptor-binding protein and blocks virus binding and entry independently of glycosaminoglycans (GAGs). The study aimed to identify the host interaction partners of ORFV ORF047.Methods: Yeast two-hybrid cDNA library of sheep testicular cells was applied to screen the host targets with ORF047 as the bait. ORF047 was cloned into a pBT3-N vector and expressed in the NMY51 yeast strain. Then, the expression of bait proteins was validated by Western blot analysis.Results: Sheep SERP1and PABPC4 were identified as host target proteins of ORFV ORF047, and a Co-IP assay further verified their interaction. Conclusions: New host cell proteins SERP1and PABPC4 were found to interact with ORFV ORF047 and might involve viral mRNA translation and replication.
Improved Gossypium raimondii Genome Using a Hi-C-based Proximity-Guided Assembly
Authors: song Guoli , Yang Qiuhong , Zuo Dongyun , Cheng Hailiang , Zhang Youping , Wang Qiaolian , Ashraf Javaria , Feng Xiaoxu , Li Simin , Chen Xiaoqin , Liu Shang 
Published: January 2021

Abstract
Introduction: Genome sequence plays an important role in both basic and applied studies. Gossypium raimondii, the putative contributor of the D subgenome of Upland cotton (G. hirsutum), highlights the need to improve the genome quality rapidly and efficiently. Methods: We performed Hi-C sequencing of G. raimondii and reassembled its genome based on a set of new Hi-C data and previously published scaffolds. We also compared the reassembled genome sequence with the previous published G. raimondii genomes for gene and genome sequence collinearity. Result: A total of 98.42% of scaffold sequence was clustered successfully, among which 99.72% of the clustered sequence was ordered and 99.92% of the ordered sequence was oriented with high-quality. Further evaluation of results by heat-map and collinearity analysis revealed that the current reassembled genome is significantly improved than the previous one (Wang et al. 2012). Conclusion: This improvement in G. raimondii genome not only provides a better reference genome to increase study efficiency but also offers a new way to assemble cotton genomes. Furthermore, Hi-C data of G. raimondii may be used for 3D structure research or regulating analysis.
Succinate dehydrogenase deficient gastrointestinal stromal tumor in a three month old boy with a fatal clinical course: A case report and review of literature
Authors: Lv Bei-bei , Li Jia-Mei , Yao Zhi-Gang , Cheng Xian-Kui , Ren Fu-Xin , Su Wen-Jing , Qin Ye-Jun , Wang Zhou , Cao Zhi-xin 
Published: January 2021

Abstract
Background: Succinate dehydrogenase deficient gastrointestinal stromal tumors (SDH-deficient GISTs), which lack KIT or PDGFRA mutations demonstrate unique clinical and pathological features, and they respond poorly to standard targeted therapy. We herein present a novel case of SDH-deficient GIST in a three-month-old infant’s colon mesentery, and he is the youngest patient to date .Case presentation: The infant presented with complaints of blood in the stool. CT showed a 6.3×4.6 cm mass in the left lower retroperitoneal. Complete resection of tumor and segmental bowel resection was performed without regional lymphadenectomy. Histologically, tumor cells were distinctive in their multinodular colon wall involvement with interspersed tracts of colon wall smooth muscle. The tumor was composed mainly of epithelioid cells. Immunohistochemically, the tumor cells were positive for Vim, CD117, PDGFR, while negative for SDHB. Mutational analysis showed a synonymous mutation for SDHB and wild-type for KIT and PDGFRA. Two months after surgery, metastases were found and Imatinib was administered. Unfortunately, the disease continued to progress, and the infant died 5 months after surgery.Conclusions: SDH-deficient GISTs comprise a subgroup of a relatively rare tumor type and show a number of clinically and biologically unique features, especially for infants. It is of great importance to developing new therapeutic targets and novel specific drugs.
Zarządzanie infrastrukturą miejską w republikańskim i cesarskim Rzymie
Authors: Kamińska Renata 
Published: January 2021

Okres przełomu republiki i pryncypatu zapisał się na kartach historii Imperium Rzymskiego jako czas wielu zmian, zarówno o charakterze ustrojowo-politycznym, jak i administracyjnym i społeczno-gospodarczym. Przeobrażenia w sposobie rządzenia państwem zaowocowały pojawieniem się nowych organów publicznych, które odebrały dotychczasowym ich znaczenie. Zmiany te wpłynęły także na strukturę, sposób rządzenia i infrastrukturę miast, a zwłaszcza Rzymu.
W przypadku administracji publicznej w zakresie infrastruktury miejskiej transformacja ustrojowa wraz ze zmianami społecznymi spowodowały, że jej funkcje w tym zakresie uległy znacznemu rozszerzeniu, a sposób ich pełnienia został dostosowany do treści i skali aktualnych potrzeb.
Konkurencyjność powództw z art. 189 k.p.c. i art. 10 u.k.w.h. (uwagi na tle wyroku Sądu Najwyższego z 23 marca 2018 r.)
Authors: Gapska Edyta 
Published: January 2021

W artykule poruszona została kwestia relacji pomiędzy powództwem o usunięcie niezgodności między treścią księgi wieczystej a rzeczywistym stanem prawnym, znajdującym podstawę normatywną w art. 10 u.k.w.h., i powództwem z art. 189 k.p.c. o ustalenie istnienia lub nieistnienia prawa lub stosunku prawnego. Autorka aprobująco odnosi się do poglądu akcentującego odmienność celów i charakteru obu tych powództw jako zmierzających do osiągnięcia innego rodzaju ochrony prawnej. Weryfikacja tego założenia pozwala także na zaaprobowanie tezy, że interesu prawnego w wytoczeniu powództwa o ustalenie na podstawie art. 189 k.p.c. nie wyklucza możliwość zgłoszenia przez powoda roszczenia z art. 10 u.k.w.h.
W ocenie autorki powyższe stanowisko nie uzasadnia jednak posługiwania się na gruncie prawa procesowego cywilnego pojęciem „zbieg roszczeń w zakresie ww. powództw” i to zarówno z przyczyn metodologicznych, jak i normatywnych. Z perspektywy wspomnianej odmienności form ochrony prawnej i sfery, w zakresie której ta ochrona ma oddziaływać, nieuprawnione jest twierdzenie, że powództwa z art. 10 u.k.w.h. i art. 189 k.p.c. są w stanie zaspokoić ten sam interes prawny.
Dietary Carbohydrates - Requirement and Recommendation in the Human Diet
Authors: Ahsan Haseeb 
Published: January 2021

Background:
Dietary carbohydrates are a group of natural compounds that includes simple
sugars and complex polysaccharides in addition to fibres. The primary role of carbohydrates is
to provide energy for various physiological functions in the body.

Objective:
A relatively high intake of complex carbohydrates and a limited intake of added or free
sugars are generally recommended for a healthy diet. Carbohydrates are essential nutrients that are
obtained from consumed food and cannot be synthesized by the body.

Methods:
The requirement of carbohydrates in diets such as sugars, starches, and fibres is essential
to understand and evaluate the nutritional status of individuals, populations, and the epidemiology
of deficiency diseases.

Results:
The dietary guidelines specify that carbohydrates should provide about half of the total
calories in a balanced diet while free sugars should be limited to 5-10% of energy. Dietary fibre is
a non-digestible carbohydrate that is not absorbed in the body but is important for satiety and laxation.
Adult humans should consume 40-60% of their daily calorie intake from carbohydrates, except
for infants and children who need a higher proportion of fats and proteins in their diet.

Conclusion:
It is recommended to consume complex carbohydrates and dietary fibres in the diet
for health. Free and added sugars are not recommended if a person is diabetic, obese, and trying to
lose weight. The function of different types of carbohydrates in the diet is an active area of study
and further recommendations are essential to elucidate its function in health and diseases.
Etiology of Neuroinflammatory Pathologies in Neurodegenerative Diseases: A Treatise
Authors: Downs Bernard W. , Kushner Steve , Bagchi Manashi , Blum Kenneth , Badgaiyan Rajendra D. , Chakraborty Sanjoy , Bagchi Debasis 
Published: January 2021

Background:Conventional medical therapies for neurodegenerative diseases primarily target anti-inflammatory interventions, immune suppression of autoimmune pathologies, and, depending on the diagnosed pathological mechanisms, neurotransmitter reuptake inhibition, among other tactics. However, the incidence of neuroinflammatory pathologies appears to be progressively increasing. The National Institutes of Health, National Institute of Environmental Health Sciences in 2016, estimated that 5.4 million Americans were living with Alzheimer's. If no effective solutions are found and implemented, within 30 years of this publication, according to data from Harvard, more than 12 million Americans will suffer from neurodegenerative diseases.Methods:Rather than investigating greater etiological depth, modern medicine seems to have been designed to addressing obvious symptomologies to relieve suffering for as long as possible until neuropathological progress inevitably wins in achieving complete functional disability and death. Researchers are reporting herein evidence-based effective treatment therapies that are outside conventional medical standard of care therapies.Conclusion:These therapies are the result of a deeper exploration into etiological factors, including an expanded understanding of the role of anaerobic pathologies in the etiology of neuroinflammatory disorders and methods of reverting to a competent aerobic metabolism. Such therapies include a liquid VMP35 MNC; a greater focus on viral mechanistic pathologies and their remission; and understanding of the genetic basis for a loss of neurological interconnectivity and consequential reward deficiencies in combination with neuronutrient deficiencies, enabling neuronutrient repletion with nutrigenomic therapies such as the KB220Z.
Prospects of Antiviral Drugs Derived from Natural Products: Targeting
SARS-CoV Entry and Replication
Authors: Soon Tsuey Ning , Yap Wei Hsum , Lim Ya Chee , Long Chiau Ming , Goh Bey Hing , Tang Yin-Quan 
Published: January 2021

:
The recent coronavirus disease (COVID-19) pandemic outbreak caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid spread from continent to continent
pose a global health emergency. Researchers are making headway to combat the ongoing COVID-19
to prevent further losses. Many natural antiviral compounds have been explored for their potential
application in treating viral infections, including those caused by SARS- and MERS-CoV. This
review focuses on natural compounds that have been showing promising results against SARS-CoV,
SARS-CoV-2 and MERS-CoV, along with their mechanism of action. The entry and replication of
CoV are among the major mechanism for the spread of COVID-19. In this context, natural
compounds inhibiting the proteins essential for SARS-CoV-2 entry and replication in the nanomolar
(nicotianamine) and micromolar (baicalin, baicalein, scutellarein, dihydromyricetin, quercetagetin,
myricetin, amentoflavone, herbacetin, isobavachalcone, quercetin 3‐β‐d‐glucoside, helichrysetin,
hirsutenone, hirsutanonol, oregonin, rubranol, rubranoside B, rubranoside A, tanshinones, emodin,
and griffithsin) concentration could be potential sources of new anti-SARS-CoV-2 drugs.