bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating Carboxysome Morphology Dynamics with a Rotationally Invariant Variational Autoencoder
Authors: Fuentes-Cabrera Miguel , Sakkos Jonathan K. , Ducat Daniel C. , Ziatdinov Maxim 
Published: November 2021

AbstractCarboxysomes are a class of bacterial microcompartments that form proteinaceous organelles within the cytoplasm of cyanobacteria and play a central role in photosynthetic metabolism by defining a cellular microenvironment permissive toCO2fixation. Critical aspects of the assembly of the carboxysomes remain relatively unknown, especially with regard to the dynamics of this microcompartment. We have recently expressed an exogenous protease as a way of gaining control over endogenous protein levels, including carboxysomal components, in the model cyanobacteriumSynechococcous elongatusPCC 7942. By utilizing this system, proteins that compose the carboxysome can be tuned in real-time as a method to examine carboxysome dynamics. Yet, analysis of subtle changes in carboxysome morphology with microscopy remains a low-throughput and subjective process. Here we use deep learning techniques, specifically a Rotationally Invariant Variational Autoencoder (rVAE), to analyze the fluorescence microscopy images and quantitatively evaluate how carboxysome shell remodelling impacts trends in the morphology of the microcompartment over time. We find that rVAEs are able to assist in the quantitative evaluation of changes in carboxysome location, shape, and size over time. We propose that rVAEs may be a useful tool to accelerate the analysis of carboxysome assembly and dynamics in response to genetic or environmental perturbation, and may be more generally useful to probe regulatory processes involving a broader array of bacterial microcompartments.
Engraftment of allotransplantated tumour cells in adult rag2 mutant Xenopus tropicalis
Authors: Tulkens Dieter , Dimitrakopoulou Dionysia , Van Nieuwenhuysen Tom , Boelens Marthe , Demuynck Suzan , Toussaint Wendy , Creytens David , Van Vlierberghe Pieter , Vleminckx Kris 
Published: November 2021

AbstractModelling human genetic diseases and cancer in lab animals has been greatly aided by the emergence of genetic engineering tools such as TALENs and CRISPR/Cas9. We have previously demonstrated the ease with which genetically engineered Xenopus models (GEXM) can be generated. This included the induction of autochthonous tumour formation by injection of early embryos with Cas9 recombinant protein loaded with sgRNAs targeting multiple tumour suppressor genes. What has been lacking so far is the possibility to propagate the induced cancers via transplantation. In this paper we describe the generation of a rag2-/- knock-out line in Xenopus tropicalis that is deficient in functional T- and B-cells. This line was validated by means of an allografting experiment with a primary tp53-/- donor tumour. In addition, we optimized available protocols for sub-lethal gamma irradiation of X. tropicalis froglets. Irradiated animals also allowed stable, albeit transient, engraftment of transplanted tp53-/- tumour cells. The novel X. tropicalis rag2-/- line and the irradiated wild type froglets will further expand the experimental toolbox in this diploid amphibian, and help to establish it as a versatile and relevant model for exploring human cancer.
Effector loss drives adaptation of Pseudomonas syringae pv. actinidiae to Actinidia arguta
Authors: Hemara Lauren M. , Jayaraman Jay , Sutherland Paul W. , Montefiori Mirco , Arshed Saadiah , Chatterjee Abhishek , Chen Ronan , Andersen Mark , Mesarich Carl H. , van der Linden Otto , Schipper Magan M. , Vanneste Joel L. , Brendolise Cyril , Templeton Matthew D. 
Published: November 2021

SummaryA pandemic isolate ofPseudomonas syringaepv.actinidiaebiovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars ofActinidia chinensis. In contrast,A. arguta(kiwiberry) is resistant to Psa3. This resistance is mediated via effector-triggered immunity, as demonstrated by induction of the hypersensitive response in infectedA. argutaleaves, observed by microscopy and quantified by ion-leakage assays. Isolates of Psa3 that cause disease inA. argutahave been isolated and analyzed, revealing a 49 kb deletion in the exchangeable effector locus (EEL). This natural EEL-mutant isolate and strains with synthetic knockouts of the EEL were more virulent inA. argutaplantlets than wild-type Psa3. Screening of a complete library of Psa3 effector knockout strains identified increased growthin plantafor knockouts of four effectors – AvrRpm1a, HopF1c, HopZ5a, and the EEL effector HopAW1a – suggesting a resistance response inA. arguta. Hypersensitive response (HR) assays indicate that three of these effectors trigger a host species-specific HR. A Psa3 strain with all four effectors knocked out escaped host recognition, but a cumulative increase in bacterial pathogenicity and virulence was not observed. These avirulence effectors can be used in turn to identify the first cognate resistance genes inActinidiafor breeding durable resistance into future kiwifruit cultivars.
Experience with open schools and preschools in periods of high community transmission of COVID-19 in Norway during the academic year of 2020/2021
Authors: Stebbings Sara , Rotevatn Torill Alise , Larsen Vilde Bergstad , Surén Pål , Elstrøm Petter , Greve-Isdahl Margrethe , Johansen Tone Bjordal , Astrup Elisabeth 
Published: November 2021

BackgroundSchools and preschools have largely remained open in Norway throughout the pandemic, with flexible mitigation measures in place. This contrasts with many other high-income countries that closed schools for long periods of time. Here we describe cases and outbreaks of COVID-19 in schools and preschools during the academic year 2020/2021, to evaluate the strategy of keeping these open with infection prevention control measures in place.MethodsIn this descriptive study, the Norwegian Institute of Public Health initiated systematic surveillance for COVID-19 cases and outbreaks in schools and preschools in October 2020. Data was compiled from the national outbreak alert system VESUV, municipality websites, and media scanning combined with the national emergency preparedness register Beredt C-19. An outbreak was defined as ≥ 2 cases among pupils or staff within 14 days at the same educational setting. Settings were categorized as preschool (1-5-years), primary school (6-12-years), lower secondary school (13-15-years) and upper secondary school (16-18-years). We reported the incidence rate among preschool and school-aged pupils and gave a descriptive overview of outbreaks and included cases per educational setting.ResultsDuring the whole academic year, a total of 1203 outbreaks in preschools and school settings were identified, out of a total of 8311 preschools and schools nationwide. The incidence of COVID-19 in preschool- and school-aged children and the rates of outbreaks in these settings largely followed the community trend. Most of the outbreaks occurred in primary schools (40%) and preschools (25%). Outbreaks across all settings were mostly small (median 3 cases, range 2 to 72), however, 40 outbreaks (3% of total) included 20 or more cases. The larger outbreaks were predominantly seen in primary schools (43%).ConclusionsWe observed few large outbreaks in open schools and preschools in Norway during the academic year of 2020/2021, also when the Alpha variant was predominant. This illustrates that it is possible to keep schools and preschools open even during periods of high community transmission of COVID-19. Adherence to targeted IPC measures adaptable to the local situation has been essential to keep educational settings open, and thus reduce the total burden on children and adolescents.
Developing a taxonomy of care coordination for people living with rare conditions: A qualitative study
Authors: Walton Holly , Simpson Amy , Ramsay Angus I.G. , Hudson Emma , Hunter Amy , Jones Jennifer , Li Ng Pei , Leeson-Beevers Kerry , Bloom Lara , Kai Joe , Kerecuk Larissa , Kokocinska Maria , Sutcliffe Alastair G , Morris Stephen , Fulop Naomi J 
Published: November 2021

AbstractBackgroundImproving care coordination is particularly important for individuals with rare conditions (who may experience multiple inputs into their care, across different providers and settings). To develop and evaluate strategies to potentially improve care coordination, it is necessary to develop a method for organising different ways of coordinating care for rare conditions. Developing a taxonomy would help to describe different ways of coordinating care and in turn facilitate development and evaluation of pre-existing and new models of care coordination for rare conditions. To the authors’ knowledge, no studies have previously developed taxonomies of care coordination for rare conditions. This research aimed to develop and refine a care coordination taxonomy for people with rare conditions.MethodsThis study had a qualitative design and was conducted in the United Kingdom. To develop a taxonomy, six stages of taxonomy development were followed. We conducted interviews (n=30 health care professionals/charity representatives/commissioners) and focus groups (n=4 focus groups, 22 patients/carers with rare/ultra-rare/undiagnosed conditions). Interviews and focus groups were audio-recorded with consent, and professionally transcribed. Findings were analysed using thematic analysis. Themes were used to develop a taxonomy, and to identify which types of coordination may work best in which situations. To refine the taxonomy, we conducted two workshops (n=12 patients and carers group; n=15 professional stakeholder group).ResultsOur taxonomy has six domains, each with different options. The six domains are: 1) Ways of organising care (local, hybrid, national), 2) Ways of organising professionals involved in care (collaboration between many or all professionals, collaboration between some professionals, a lack of collaborative approach), 3) Responsibility for coordination (administrative support, formal roles and responsibilities, supportive roles and no responsibility), 4) How often appointments and coordination take place (regular, on demand, hybrid), 5) Access to records (full or filtered access), and 6) Mode of care coordination (face-to-face, digital, telephone).ConclusionsFindings indicate that there are different ways of coordinating care across the six domains outlined in our taxonomy. This may help to facilitate the development and evaluation of existing and new models of care coordination for people living with rare conditions.
Discovery of key function module in complex mechanical 3D CAD assembly model for design reuse
Authors: Han Zhoupeng , Tian Chenkai , Zhou Zihan , Yuan Qilong 
Published: November 2021

PurposeComplex mechanical 3D computer-aided design (CAD) model embodies rich implicit design knowledge. Through discovering the key function parts and key function module in 3D CAD assembly model in advance, it can promote the designers’ understanding and reuse efficiency of 3D assembly model in design reuse.Design/methodology/approachAn approach for discovering key function module in complex mechanical 3D CAD assembly model is proposed. First, assembly network for 3D CAD assembly model is constructed, where the topology structure characteristics of 3D assembly model are analyzed based on complex network centrality. The degree centrality, closeness centrality, betweenness centrality and mutual information of node are used to evaluate the importance of the parts in 3D assembly model. Then, a multi-attribute decision model for part-node importance is established, and the comprehensive evaluation for key function parts in 3D assembly model is accomplished by combining Analytic Hierarchy Process and Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS). Subsequently, a community discovery of function module in assembly model-based Clauset–Newman–Moore (CNM)-Centrality is given in details. Finally, 3D CAD assembly model of worm gear reducer is taken as an example to verify the effectiveness and feasibility of proposed method.FindingsThe key function part in CAD assembly model is evaluated comprehensively considering assembly topology more objectively. In addition, the key function module containing key function part is discovered from CAD assembly model by using CNM-Centrality-based community discovery.Practical implicationsThe approach can be used for discovering important design knowledge from complex CAD assembly model when reusing the assembly model. It can help designers capture and understand the design thinking and intent, improve the reuse efficiency and quality.Originality/valueThe paper first proposes an approach for discovering key function module in complex mechanical 3D CAD assembly model taking advantage of complex network theory, where the key function part is evaluated using node centrality and TOPSIS, and the key function module is identified based on community discovery.
Practices of poverty alleviation by open and distance education: a case report from the Open University of China
Authors: Ju Chuanjin , Hou Songyan , Shao Dandan , Zhang Zhijun , Yu Zhangli 
Published: November 2021

PurposeThe purpose of this report is to demonstrate open and distance education (ODE) can support poverty alleviation. Taking the practices of the Open University of China (the OUC) as an example, this paper aims to reveal how open universities make contributions to local residents in rural and remote areas.Design/methodology/approachFocusing on 25 poverty-stricken counties, the OUC had invested 58 million RMB to its learning centers in these counties from 2017 to 2020. The first one is to improve ICT and educational facilities in these learning centers. The second approach is to cultivate local residents with degree programs through ODE so as to promote local economic development. The third one is to design and develop training programs according to local context to meet the specific needs of local villagers.FindingsAfter 3 years working, cloud-based classrooms and computer rooms have been set up. Bookstores have been founded and printed books have been donated. Hundreds of thousands of digital micro lectures have been supplied to these learning centers which have been improved and fully played their functions. Nearly 50,000 local residents have been directly benefited. Village leaders have helped lift local residents out of poverty. Poverty-stricken villagers have been financed to study on either undergraduate or diploma programs. Local residents have improved their skills by learning with the training programs offered by the OUC.Originality/valueODE is proved to be an effective way to eradicate poverty. Open universities are proved to be able to make contributions to social justice. By fulfilling its commitments to eliminate poverty within the national strategy framework, the OUC has built its brand nationwide.
Relating supervisor interpersonal emotion management- and task-oriented leadership to adaptive performance: a moderated-mediation model incorporating trust and gender
Authors: Adams Gary A. , Webster Jennica R. 
Published: November 2021

PurposeThe authors incorporated leadership and gender theories with research on trust to propose a model relating interpersonal emotion management (IEM, a type of relational leadership) and task-oriented (T-O) leadership to follower adaptive performance. The authors also examine the indirect effect of IEM and T-O on adaptive performance via trust and the possible moderating role of gender on these relationships.Design/methodology/approachThe authors tested this model using a sample of 314 workers who rated their direct leaders (supervisors).FindingsOverall, results supported the model for IEM as it was directly and indirectly related (via trust) to adaptive job performance (even after controlling for transformational leadership) and these relationships were more positive for women leaders. T-O leadership was related to adaptive job performance as expected but was unrelated to trust or, via trust, to adaptive performance. Findings also suggest that women direct leaders may garner more trust and adaptive performance from followers by engaging in higher levels of IEM, while also not experiencing backlash for engaging in the more agentic T-O behaviors during a crisis.Practical implicationsDespite an emphasis on women's relational leadership during a crisis, the authors findings show organizations are best served by ambidextrous leaders who can manage the emotions and tasks of their followers and that both women and men can engage in these leadership styles without penalty.Originality/valueMuch research regarding women's leadership advantage during a crisis is based on political leaders or has been conducted in lab settings. Further, it has focused on attitudes toward the women leaders rather than their performance. Research has also not considered both IEM along with the possible backlash women may experience for engaging in T-O leadership.
Explaining resistance intention towards mobile HRM application: the dark side of technology adoption
Authors: Shankar Amit , Nigam Achint 
Published: November 2021

PurposeThe mobile human resource management application (mHRM app) has recently been seen as an innovative cloud-based solution to manage human resource management (HRM) within organisation. Despite its great potential, organisations have shown resistance towards the usage of the mHRM app. This study investigates the dark side of electronic HRM (eHRM) by examining factors affecting HR professionals' resistance to the mHRM app using status quo bias (SQB) theory. The study also examines the moderating effect of personal innovativeness.Design/methodology/approachResponses were collected from 239 HR professionals using an online survey. Structural equation modelling (SEM) and PROCESS macro were used to examine the hypotheses.FindingsThe results indicated that regret avoidance, inertia, switching costs and perceived threat significantly affect HR professionals' resistance towards mHRM app adoption. Results also indicated that high personal innovativeness negatively moderates the association between inhibitors and resistance to adopt the mHRM app.Practical implicationsThe study's findings will help HR professionals reduce their resistance towards mHRM app adoption.Originality/valueThis study enriches eHRM, mobile applications and the SQB literature.
Understanding public service motivation, workplace spirituality and employee well-being in the public sector
Authors: Hassan Sobia , Ansari Nighat , Rehman Ali , Moazzam Amani 
Published: November 2021

Purpose
The public service motivation (PSM) theory implies that public employees are spiritual people called “public servants” who have a desire to affect the community and are characterized by compassion and serving others. Owing to their commitment to public welfare, spirituality is apparently inherited in public employees as an occupation/employment effort, which entails attaching a “meaning” to the work being done in the workplace for the spiritual satisfaction of the employees. A sense of well-being among the employees of an organization can prove instrumental in developing their motivation level and improving the quality of their services. The literature depicts that workplace spirituality (WPS) is a feature that enhances multiple forms of employee well-being (EWB). Considering the importance of these concepts in terms of enhancing the productivity of the organizations, the current study aimed to gain an understanding of the PSM together with two other positive attributes namely WPS and EWB and determine their interrelationship. The aim of this study is to examine a significant positive relationship between PSM and WPS mediated by EWB.

Design/methodology/approach
The study has been conducted in the context of the public sector of Pakistan where a sample of the academic staff of higher educational institutions in the Punjab province was selected through probability sampling techniques for conducting the survey. The data collected from 394 respondents from the selected faculties of the universities were analyzed using relevant statistical tools (SPSS and AMOS: 22) to answer the research questions.

Findings
This study supported a significant positive relationship between PSM and WPS mediated by EWB. The quantitative findings of this study, thus, demonstrated that the culture of spirituality in the workplace significantly affects the PSM of employees by way of creating a sense of well-being among the employees.

Originality/value
This study is unique as it serves as an effort to understand the spiritual experience of the public sector employees involved in public service. This infers that spirituality in the workplace improved employees’ well-being by giving them a strong sense of purpose and these employees in a healthy state of mind are more likely to perform above and beyond and have a high motivation to serve the public.
Spaces of learning – practising the SDGs through geographical fieldwork methods in a nature park
Authors: Grindsted Thomas Skou , Nielsen Thomas Theis 
Published: November 2021

PurposeWhile the sustainable development goals (SDGs) and visions for sustainability education apply to many methods, they can be hard to put into practice. This study aims to concern an undergraduate geography course designed not only to teach geographical methods but also to engage with the multi-scalar nature of the SDGs and apply them to various local urban sustainability issues in a real-world context.Design/methodology/approachBy means of a mixed-method approach, the authors examine a fieldwork course that invites students into learning situations in which they combine critical thinking with entrepreneurial solutions to local sustainability challenges. The authors examine the learning material from the students’ cases and explore the geographical knowledge the students’ practise.FindingsFieldwork helps students contextualise the multi-scalar nature of the SDGs and thereby apply them to analyses in a local context. Students learn first-hand how their planning proposals can be seen as counterproductive by some local stakeholders while remaining attractive to others.Originality/valueStudent tasks are developed in collaboration with a local municipality and students present their findings to local politicians and stakeholders. Presenting and localising the SDGs within a local community not only encourages students to undertake a local community analysis but also provides new perspectives to local stakeholders.