bluuebunny/crossref_metadata_2025_split huggingface dataset:
Secreted Wnt antagonists in scrub typhus
Authors: Ueland Thor , Astrup Elisabeth , Otterdal Kari , Lekva Tove , Janardhanan Jeshina , Prakash John A. J. , Thomas Kurien , Michelsen Annika E. , Aukrust Pål , Varghese George M. , Damås Jan K. 
Published: April 2021

Background
The mechanisms that control local and systemic inflammation in scrub typhus have only been partially elucidated. The wingless (Wnt) signaling pathways are emerging as important regulators of inflammation and infection, but have not been investigated in scrub typhus.

Methodology/Principal findings
Plasma levels of secreted Wnt antagonists (i.e. DKK-1, sFRP-3, WIF-1 and SOST) were analyzed in patients with scrub typhus (n = 129), patients with similar febrile illness without O. tsutsugamushi infection (n = 31), febrile infectious disease controls, and in healthy controls (n = 31) from the same area of South India, and were correlated to markers of inflammation, immune and endothelial cell activation as well as for their association with organ specific dysfunction and mortality in these patients. We found i) Levels of SOST and in particular sFRP-3 and WIF-1 were markedly increased and DKK-1 decreased in scrub typhus patients at admission to the hospital compared to healthy controls. ii) In recovering scrub typhus patients, SOST, sFRP-3 and WIF-1 decreased and DKK-1 increased. iii) SOST was positively correlated with markers of monocyte/macrophage and endothelial/vascular activation as well as with renal dysfunction and poor outcome iv) Finally, regulation of Wnt pathways by O. tsutsugamushi in vitro in monocytes and ex vivo in mononuclear cells isolated from patients with scrub typhus, as evaluated by gene expression studies available in public repositories, revealed markedly attenuated canonical Wnt signaling.

Conclusions/Significance
Our findings suggest that scrub typhus is characterized by attenuated Wnt signaling possibly involving dysregulated levels of several secreted pathway antagonists. The secreted Wnt antagonist SOST was strongly associated with renal dysfunction and poor prognosis in these patients.
Routine deworming during antenatal care decreases risk of neonatal mortality and low birthweight: A retrospective cohort of survey data
Authors: Walia Bhavneet , Kmush Brittany L. , Lane Sandra D. , Endy Timothy , Montresor Antonio , Larsen David A. 
Published: April 2021

Background
Soil transmitted helminths (STH) are a common infection among pregnant women in areas with poor access to sanitation. Deworming medications are cheap and safe; however, the health benefit of deworming during pregnancy is not clear.

Methods / Principal findings
We created a retrospective cohort of more than 800,000 births from 95 Demographic and Health Survey datasets to estimate the impact of deworming medicine during routine antenatal care (ANC) on neonatal mortality and low birthweight. We first matched births on the probability of receiving deworming during ANC. We then modeled the birth outcomes with the matched group as a random intercept to estimate the effect of deworming during antenatal care after accounting for various risk factors. We also tested for effect modification of soil transmitted helminth prevalence on the impact of deworming during ANC. Receipt of deworming medication during ANC was associated with a 14% reduction in the risk of neonatal mortality (95% confidence interval = 10–17%, n = 797,772 births), with no difference between high and low transmission countries. In low transmission countries, we found an 11% reduction in the odds of low birth weight (95% confidence interval = 8–13%) for women receiving deworming medicine, and in high transmission countries, we found a 2% reduction in the odds of low birthweight (95% confidence interval = 0–5%).

Conclusions / Significance
These results suggest a substantial health benefit for deworming during ANC that may be even greater in countries with low STH transmission.
Immunization by exposure to live virus (SIVmne/HIV-2287) during antiretroviral drug prophylaxis may reduce risk of subsequent viral challenge
Authors: Frenkel Lisa M. , Kuller LaRene , Beck Ingrid A. , Tsai Che-Chung , Joy Jaimy P. , Mulvania Thera M. , Hu Shiu-Lok , Montefiori David C. , Anderson David M. 
Published: April 2021

Rationale/Study designA major challenge in the development of HIV vaccines is finding immunogens that elicit protection against a broad range of viral strains. Immunity to a narrow range of viral strains may protect infants of HIV-infected women or partners discordant for HIV. We hypothesized that immunization to the relevant viral variants could be achieved by exposure to infectious virus during prophylaxis with antiretroviral drugs. To explore this approach in an animal model, macaques were exposed to live virus (SIVmneor HIV-2287) during prophylaxis with parenteral tenofovir and humoral and cellular immune responses were quantified. Subsequently, experimental animals were challenged with homologous virus to evaluate protection from infection, and if infection occurred, the course of disease was compared to control animals. Experimental animals uninfected with SIVmnewere challenged with heterologous HIV-2287to assess resistance to retroviral infection.Methodology/Principal findingsJuvenile femaleMacaca nemestrina(N = 8) were given ten weekly intravaginal exposures with either moderately (SIVmne) or highly (HIV-2287) pathogenic virus during tenofovir prophylaxis. Tenofovir protected all 8 experimental animals from infection, while all untreated control animals became infected. Specific non-neutralizing antibodies were elicited in blood and vaginal secretions of experimental animals, but no ELISPOT responses were detected. Six weeks following the cessation of tenofovir, intravaginal challenge with homologous virus infected 2/4 (50%) of the SIVmne-immunized animals and 4/4 (100%) of the HIV-2287-immunized animals. The two SIVmne-infected and 3 (75%) HIV-2287-infected had attenuated disease, suggesting partial protection.Conclusions/SignificanceRepeated exposure to SIVmneor HIV-2287, during antiretroviral prophylaxis that blocked infection, induced binding antibodies in the blood and mucosa, but not neutralizing antibodies or specific cellular immune responses. Studies to determine whether antibodies are similarly induced in breastfeeding infants and sexual partners discordant for HIV infection and receiving pre-exposure antiretroviral prophylaxis are warranted, including whether these antibodies appear to confer partial or complete protection from infection.
High burden of tuberculosis infection and disease among people receiving medication-assisted treatment for substance use disorder in Tanzania
Authors: Minja Lilian Tina , Hella Jerry , Mbwambo Jessie , Nyandindi Cassian , Omary Ubuguyu Said , Levira Francis , Mpagama Stellah , Shimwela Meshack , Okuma James , Gagneux Sebastien , Bruce R. Douglas , Reither Klaus 
Published: April 2021

Objective
To determine the prevalence of tuberculosis (TB) disease and infection as well as incident TB disease among people who use drugs (PWUD) attending Medication Assisted Treatment (MAT) clinics in Dar-es-Salaam, Tanzania.

Methods
In this prospective cohort study, a total of 901 consenting participants were enrolled from November 2016 to February 2017 and a structured questionnaire administered to them through the open data kit application on android tablets. Twenty-two months later, we revisited the MAT clinics and reviewed 823 of the 901 enrolled participant’s medical records in search for documentation on TB disease diagnosis and treatment. Medical records reviewed included those of participants whom at enrolment were asymptomatic, not on TB disease treatment, not on TB preventive therapy and those who had a documented tuberculin skin test (TST) result.

Results
Of the 823 medical records reviewed 22 months after enrolment, 42 had documentation of being diagnosed with TB disease and initiated on TB treatment. This is equivalent to a TB disease incidence rate of 2,925.2 patients per 100,000 person years with a total follow up time of 1,440 person-years. At enrolment the prevalence of TB disease and TB infection was 2.6% and 54% respectively and the HIV prevalence was 44% and 16% among females and males respectively.

Conclusion
PWUD attending MAT clinics bear an extremely high burden of TB and HIV and are known to have driven TB epidemics in a number of countries. Our reported TB disease incidence is 12 times that of the general Tanzanian incidence of 237 per 100,000 further emphasizing that this group should be prioritized for TB screening, testing and treatment. Gender specific approaches should also be developed as female PWUDs are markedly more affected with HIV and TB disease than male PWUDs.
Multidrug resistance pattern of Acinetobacter species isolated from clinical specimens referred to the Ethiopian Public Health Institute: 2014 to 2018 trend anaylsis
Authors: Ayenew Zeleke , Tigabu Eyasu , Syoum Elias , Ebrahim Semira , Assefa Dawit , Tsige Estifanos 
Published: April 2021

BackgroundAcinetobacterspecies have been a leading cause of nosocomial infections, causing significant morbidity and mortality over the entire world including Ethiopia. The most important features ofA.baumanniiare its ability to persist in the hospital environment and rapidly develop resistance to a wide variety of antibiotics. This study aimed to determine trend of antimicrobial resistance inAcinetobacterspecies over a five years period.MethodA retrospective data regarding occurrence and antimicrobial resistance ofAcinetobacterspecies recovered from clinical specimens referred to the national reference laboratory was extracted from microbiology laboratory data source covering a time range from 2014 to 2018. Socio-demographic characteristics and laboratory record data was analyzed using SPSS 20.ResultsA total of 102 strains ofAcinetobacterspecies were analyzed from various clinical specimens. Majority of them were from pus (33.3%) followed by blood (23.5%), urine (15.6%) and body fluid (11.7%). Significant ascending trends of antimicrobial resistance was shown for meropenem (12.5% to 60.7%), ceftazidime (82.1% to 100%), ciprofloxacin (59.4% to 74.4%), ceftriaxone (87.1% to 98.6%), cefepime (80.0% to 93.3%) and pipracillin- tazobactam (67.8% to 96.3%). However, there was descending trend of antimicrobial resistance for tobramycin (56.5% to 42.8%), amikacin (42.1% to 31.4%) and trimethoprim-sulfamethoxazole (79.0 to 68.2%). The overall rate of carbapenem non-susceptible and multidrug resistance rates inAcinetobacterspecies were 56.7% and 71.6%.respectively.ConclusionA five year antimicrobial resistance trend analysis ofAcinetobacterspecies showed increasing MDR and resistance to high potent antimicrobial agents posing therapeutic challenge in our Hospitals and health care settings. Continuous surveillance and appropriate infection prevention and control strategies need to be strengthened to circumvent the spread of multidrug resistant pathogens in health care facilities.
Rights based approaches to sexual and reproductive health in low and middle-income countries: A systematic review
Authors: McGranahan Majel , Nakyeyune Joselyn , Baguma Christopher , Musisi Nakibuuka Noor , Nsibirwa Derrick , Sekalala Sharifah , Oyebode Oyinlola 
Published: April 2021

IntroductionThe Sustainable Development Goals, which are grounded in human rights, involve empowering women and girls and ensuring that everyone can access sexual and reproductive health and rights (Goal 5). This is the first systematic review reporting interventions involving rights-based approaches for sexual and reproductive health issues including gender-based violence, maternity, HIV and sexually transmitted infections in low and middle-income countries.AimsTo describe the evidence on rights-based approaches to sexual and reproductive health in low and middle-income countries.MethodsEMBASE, MEDLINE and Web of Science were searched until 9/1/2020. Inclusion criteria were:Study design: any interventional study.Population: females aged over 15 living in low and middle-income countries.Intervention: a “rights-based approach” (defined by the author) and/or interventions that the author explicitly stated related to "rights".Comparator: clusters in which no intervention or fewer components of an intervention were in place, or individuals not exposed to interventions, or exposed to fewer intervention components.Outcome: Sexual and reproductive health related outcomes.A narrative synthesis of included studies was undertaken, and outcomes mapped to identify evidence gaps.The systematic review protocol was registered on PROSPERO (CRD42019158950).ResultsDatabase searching identified 17,212 records, and 13,404 studies remained after de-duplication. Twenty-four studies were included after title and abstract, full-text and reference-list screening by two authors independently.Rights-based interventions were effective for some included outcomes, but evidence was of poor quality. Testing uptake for HIV and/or other sexually transmitted infections, condom use, and awareness of rights improved with intervention, but all relevant studies were at high, critical or serious risk of bias. No study included gender-based violence outcomes.ConclusionConsiderable risk of bias in all studies means results must be interpreted with caution. High-quality controlled studies are needed urgently in this area.
Feasibility of investigating the association between bacterial pathogens and oral leukoplakia in low and middle income countries: A population-based pilot study in India
Authors: Shridhar Krithiga , Aggarwal Aastha , Rawal Ishita , Gupta Ruby , Masih Shet , Mehrotra Ravi , Gillespie Theresa W. , Dhillon Preet K. , Michaud Dominique S. , Prabhakaran Dorairaj , Goodman Michael 
Published: April 2021

Background
Certain oral bacterial pathogens may play a role in oral carcinogenesis. We assessed the feasibility of conducting a population-based study in India to examine the distributions and levels of Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia in relation to oral leukoplakia (a potentially malignant disorder) and other participant characteristics.

Methods
This exploratory case-control study was nested within a large urban Indian cohort and the data included 22 men and women with oral leukoplakia (cases) and 69 leukoplakia-free controls. Each participant provided a salivary rinse sample, and a subset of 34 participants (9 cases; 25 controls) also provided a gingival swab sample from keratinized gingival surface for quantitative polymerase chain reaction (qPCR).

Results
Neither the distribution nor the levels of pathogens were associated with oral leukoplakia; however, individual pathogen levels were more strongly correlated with each other in cases compared to controls. Among controls, the median level of total pathogens was the highest (7.55×104 copies/ng DNA) among persons of low socioeconomic status. Salivary rinse provided better DNA concentration than gingival swab for qPCR analysis (mean concentration: 1.8 ng/μl vs. 0.2 ng/μl).

Conclusions
This study confirms the feasibility of population studies evaluating oral microbiome in low-resource settings and identifies promising leads for future research.
More than ‘pour-and-mix’ – Extending Content Knowledge at the college level through an analysis of coumarin in cinnamon
Authors: Emden Markus , Scholz Matthias 
Published: April 2021

Abstract
The article introduces the layout of a college course on analytical chemistry for pre-service teachers. The course aims at fostering teacher students’ professional knowledge by recapitulating Core Content Knowledge, introducing Specialized Content Knowledge, and complementing it with discipline-overarching Linked Content Knowledge. Synthesizing these types of knowledge is expected to support students in their processes of meaning making. As they realize an every-day relevance of analytical chemistry they will see opportunities to relate chemical knowledge to their later school students. Regarding Linked Content Knowledge, suggestions are given to address aspects related to the Nature of Science. An exemplary lab activity to analyze the coumarin content in cinnamon is provided. Coumarin has shown adverse health effects in a non-neglectable proportion of humans. Regarding cinnamon there are two varieties of cinnamon with significantly different coumarin content: cassia is very rich in coumarin while Ceylon cinnamon is nearly void of it. The more popular cassia has been shown to be so high in coumarin that a potential risk for light weight consumers cannot be ruled out. Regarding school science, the lab activity can raise an awareness with students for the tentative, creative and socio-cultural Natures of Science – right from the students’ kitchen cabinets.
The effect of cognitive behavior therapy on body image and self-esteem in female adolescents: a systematic review and meta-analysis
Authors: Zamiri-Miandoab Nasrin , Hassanzadeh Robab , Kamalifard Mahin , Mirghafourvand Mojgan 
Published: April 2021

AbstractLow self-esteem and negative body image expose girls to many risks and damages. Cognitive-behavior counseling is one of the ways of improving body image and self-esteem. The aim of this systematic review was to determine the effects of cognitive-behavior therapy on body image and self-esteem of adolescent girls. The English (Cochrane library, Web of sciences, EBSCO, PubMed, Google Scholar) and Persian (SID, MagIran) databases were searched without any time limit. The quality of included studies in terms of risk of bias was assessed using Cochran handbook and the quality of evidence was evaluated through Grading of Recommendations Assessment, Development and Evaluation (GRADE). Meta-analysis was performed using RevMan software. Heterogeneity of studies was analyzed by indexI2. Subgroup analysis was performed on the basis of outcome evaluation intervals (after intervention, one month and two months later). A total of 2,664 articles were accessed in different databases of which 2,655 articles were excluded and finally nine studies were systematically reviewed. Meta-analysis results on seven studies (228 participants) showed that the group receiving cognitive-behavior counseling was significantly better than the control group regarding body image (Standardized Mean Difference [SMD]: 13.01; 95% CI: 10.68 to 15.34;I²=26.1%). Likewise, meta-analysis results on two studies (50 participants) showed that self-esteem was not significantly different between the group receiving cognitive-behavior counseling and the control group (SMD: 1.13; 95% CI: −0.7 to 2.32,I²=73%). Meta-analysis results of this study represent effectiveness of cognitive-behavior therapy on body image of adolescent girls; however, since the number of studies in the field of self-esteem is low, more trials in this field with stronger designs are suggested.
Graphical structure of extended b-metric spaces: an application to the transverse oscillations of a homogeneous bar
Authors: Younis Mudasir , Singh Deepak , Altun Ishak , Chauhan Varsha 
Published: April 2021

Abstract
The purpose of this article is to present the notion of graphical extended b-metric spaces, blending the concepts of graph theory and metric fixed point theory. We discuss the structure of an open ball of the new proposed space and elaborate on the newly introduced ideas in a novel way by portraying suitably directed graphs. We also provide some examples in graph structure to show that our results are sharp as compared to the results in the existing state-of-art. Furthermore, an application to the transverse oscillations of a homogeneous bar is entrusted to affirm the applicability of the established results. Additionally, we evoke some open problems for enthusiastic readers for the future aspects of the study.
Public Libraries and Development across Sub-Saharan Africa: Overcoming a Problem of Perception
Authors: Young Jason C. , Lynch Renee , Boakye-Achampong Stanley , Jowaisas Chris , Sam Joel 
Published: April 2021

AbstractPublic libraries and development organizations share many common goals that make them strong potential partners in the Global South. In spite of these commonalities, libraries are often overlooked as development partners. This is unfortunate because these partnerships could allow librarians to more fully and effectively participate in the development of their communities. This paper explores strategies that libraries might employ to make themselves more visible to development organizations. It does so through analysis of interviews with practitioners who have funded or implemented development projects across countries in Africa. We find that these practitioners are open to working more with libraries, but that it will take a lot of work on the part of public libraries to accomplish broad shifts in perception amongst development agencies. Collecting and openly sharing data on the output and impact of public libraries is a first step in this work, but these efforts must also be augmented through increased marketing and networking by libraries. Libraries need to build internal capacity to use data to drive advocacy efforts and attract funding from development donors.
Student Experiences with Digital Citizenship: A Comparative Cultural Study
Authors: Kammer Jenna , Atiso Kodjo , Borteye Edward Mensah 
Published: April 2021

Abstract
This comparative cultural study examines differences in digital citizenship between undergraduate information literacy students at two different, but similar, universities across the globe from each other. Under the notion that the internet and prevalence of mobile devices allow students to participate online as digital citizens in ways that were impossible before, we use mixed methods to compare the attitudes and experiences of undergraduate students at a university in the midwestern United States (U.S.), with a university on the southwestern coast of Ghana. We also examine the policies related to technology use at these schools. The findings indicate that Ghanaian students had higher levels of digital citizenship. Other findings suggest that network issues are a problem for students in both schools, especially for Ghana, and ethical aspects of internet use, like cyberbullying, hacking, and fake news, deter students from participating online as much as they would like.
Do dependency lengths explain constraints on crossing dependencies?
Authors: Yadav Himanshu , Husain Samar , Futrell Richard 
Published: April 2021

Abstract
In syntactic dependency trees, when arcs are drawn from syntactic heads to dependents, they rarely cross. Constraints on these crossing dependencies are critical for determining the syntactic properties of human language, because they define the position of natural language in formal language hierarchies. We study whether the apparent constraints on crossing syntactic dependencies in natural language might be explained by constraints on dependency lengths (the linear distance between heads and dependents). We compare real dependency trees from treebanks of 52 languages against baselines of random trees which are matched with the real trees in terms of their dependency lengths. We find that these baseline trees have many more crossing dependencies than real trees, indicating that a constraint on dependency lengths alone cannot explain the empirical rarity of crossing dependencies. However, we find evidence that a combined constraint on dependency length and the rate of crossing dependencies might be able to explain two of the most-studied formal restrictions on dependency trees: gap degree and well-nestedness.