bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular subclassification of gastrointestinal cancers based on cancer stem cell traits
Authors: Li Mei-Mei , Yuan Jun , Guan Xin-Yuan , Ma Ning-Fang , Liu Ming 
Published: November 2021

AbstractHuman gastrointestinal malignancies are highly heterogeneous cancers. Clinically, heterogeneity largely contributes to tumor progression and resistance to therapy. Heterogeneity within gastrointestinal cancers is defined by molecular subtypes in genomic and transcriptomic analyses. Cancer stem cells (CSCs) have been demonstrated to be a major source of tumor heterogeneity; therefore, assessing tumor heterogeneity by CSC trait-guided classification of gastrointestinal cancers is essential for the development of effective therapies. CSCs share critical features with embryonic stem cells (ESCs). Molecular investigations have revealed that embryonic genes and developmental signaling pathways regulating the properties of ESCs or cell lineage differentiation are abnormally active and might be oncofetal drivers in certain tumor subtypes. Currently, multiple strategies allow comprehensive identification of tumor subtype-specific oncofetal signatures and evaluation of subtype-specific therapies. In this review, we summarize current knowledge concerning the molecular classification of gastrointestinal malignancies based on CSC features and elucidate their clinical relevance. We also outline strategies for molecular subtype identification and subtype-based therapies. Finally, we explore how clinical implementation of tumor classification by CSC subtype might facilitate the development of more effective personalized therapies for gastrointestinal cancers.
RNA-seq driven expression and enrichment analysis to investigate CVD genes with associated phenotypes among high-risk heart failure patients
Authors: Ahmed Zeeshan , Zeeshan Saman , Liang Bruce T. 
Published: November 2021

Abstract
Background
Heart failure (HF) is one of the most common complications of cardiovascular diseases (CVDs) and among the leading causes of death in the US. Many other CVDs can lead to increased mortality as well. Investigating the genetic epidemiology and susceptibility to CVDs is a central focus of cardiology and biomedical life sciences. Several studies have explored expression of key CVD genes specially in HF, yet new targets and biomarkers for early diagnosis are still missing to support personalized treatment. Lack of gender-specific cardiac biomarker thresholds in men and women may be the reason for CVD underdiagnosis in women, and potentially increased morbidity and mortality as a result, or conversely, an overdiagnosis in men. In this context, it is important to analyze the expression and enrichment of genes with associated phenotypes and disease-causing variants among high-risk CVD populations.

Methods
We performed RNA sequencing focusing on key CVD genes with a great number of genetic associations to HF. Peripheral blood samples were collected from a broad age range of adult male and female CVD patients. These patients were clinically diagnosed with CVDs and CMS/HCC HF, as well as including cardiomyopathy, hypertension, obesity, diabetes, asthma, high cholesterol, hernia, chronic kidney, joint pain, dizziness and giddiness, osteopenia of multiple sites, chest pain, osteoarthritis, and other diseases.

Results
We report RNA-seq driven case–control study to analyze patterns of expression in genes and differentiating the pathways, which differ between healthy and diseased patients. Our in-depth gene expression and enrichment analysis of RNA-seq data from patients with mostly HF and other CVDs on differentially expressed genes and CVD annotated genes revealed 4,885 differentially expressed genes (DEGs) and regulation of 41 genes known for HF and 23 genes related to other CVDs, with 15 DEGs as significantly expressed including four genes already known (FLNA, CST3, LGALS3, and HBA1) for HF and CVDs with the enrichment of many pathways. Furthermore, gender and ethnic group specific analysis showed shared and unique genes between the genders, and among different races. Broadening the scope of the results in clinical settings, we have linked the CVD genes with ICD codes.

Conclusions
Many pathways were found to be enriched, and gender-specific analysis showed shared and unique genes between the genders. Additional testing of these genes may lead to the development of new clinical tools to improve diagnosis and prognosis of CVD patients.
Validation of the French ADNM-20 in the assessment of emotional difficulties resulting from COVID-19 quarantine and outbreak
Authors: Vancappel A. , Jansen E. , Bachem R. , Bray A. , Egreteau L. , Réveillère C. , Maercker A. , El-Hage W. 
Published: November 2021

Abstract
Background
Multiple psychological consequences of the COVID-19 outbreak and quarantine have been described. However, there is a lack of global conceptualization. We argue that the stressful aspects of the situation, the multiple environmental consequences of the outbreak, and the diversity of symptoms observed in such a situation, suggest that Adjustment disorder (AD) is a promising way to conceptualize the psychological consequences of the outbreak and quarantine. The first aim of the study was to validate the French version of the ADNM. The second aim was to set out adjustment difficulties resulting from COVID-19 outbreak and quarantine.

Method
We recruited 1010 (840 women, 170 men) who consented online to participate. They filled out the French ADNM, visual analogic scales, HADS, IES, and the COPE, to evaluate coping strategies.

Results
We confirmed the factor structure of the ADNM and we found good psychometric properties. We found that 61.3% of participants presented an adjustment disorder related to COVID-19 outbreak. We found multiple risk factors and protective factors to AD due to quarantine and outbreak. We also identified the coping strategies negatively and positively associated with AD.

Conclusion
Adjustment disorder is a relevant concept to understand psychological manifestations caused by quarantine and outbreak. The French ANDM has good psychometric properties to evaluate such manifestations. The association between coping strategies and AD symptoms suggest that CBT may be the best intervention to help people suffering from AD.
Roads constrain movement across behavioural processes in a partially migratory ungulate
Authors: Passoni Gioele , Coulson Tim , Ranc Nathan , Corradini Andrea , Hewison A. J. Mark , Ciuti Simone , Gehr Benedikt , Heurich Marco , Brieger Falko , Sandfort Robin , Mysterud Atle , Balkenhol Niko , Cagnacci Francesca 
Published: November 2021

AbstractBackgroundHuman disturbance alters animal movement globally and infrastructure, such as roads, can act as physical barriers that impact behaviour across multiple spatial scales. In ungulates, roads can particularly hamper key ecological processes such as dispersal and migration, which ensure functional connectivity among populations, and may be particularly important for population performance in highly human-dominated landscapes. The impact of roads on some aspects of ungulate behaviour has already been studied. However, potential differences in response to roads during migration, dispersal and home range movements have never been evaluated. Addressing these issues is particularly important to assess the resistance of European landscapes to the range of wildlife movement processes, and to evaluate how animals adjust to anthropogenic constraints.MethodsWe analysed 95 GPS trajectories from 6 populations of European roe deer (Capreolus capreolus) across the Alps and central Europe. We investigated how roe deer movements were affected by landscape characteristics, including roads, and we evaluated potential differences in road avoidance among resident, migratory and dispersing animals (hereafter, movement modes). First, using Net Squared Displacement and a spatio-temporal clustering algorithm, we classified individuals as residents, migrants or dispersers. We then identified the start and end dates of the migration and dispersal trajectories, and retained only the GPS locations that fell between those dates (i.e., during transience). Finally, we used the resulting trajectories to perform an integrated step selection analysis.ResultsWe found that roe deer moved through more forested areas during the day and visited less forested areas at night. They also minimised elevation gains and losses along their movement trajectories. Road crossings were strongly avoided at all times of day, but when they occurred, they were more likely to occur during longer steps and in more forested areas. Road avoidance did not vary among movement modes and, during dispersal and migration, it remained high and consistent with that expressed during home range movements.ConclusionsRoads can represent a major constraint to movement across modes and populations, potentially limiting functional connectivity at multiple ecological scales. In particular, they can affect migrating individuals that track seasonal resources, and dispersing animals searching for novel ranges.
Practical kidney dosimetry in peptide receptor radionuclide therapy using [177Lu]Lu-DOTATOC and [177Lu]Lu-DOTATATE with focus on uncertainty estimates
Authors: Staanum Peter Frøhlich , Frellsen Anders Floor , Olesen Marie Louise , Iversen Peter , Arveschoug Anne Kirstine 
Published: November 2021

Abstract
Background
Kidney dosimetry after peptide receptor radionuclide therapy using 177Lu-labelled somatostatin analogues is a procedure with multiple steps. We present the SPECT/CT-based implementation at Aarhus University Hospital and evaluate the uncertainty of the various steps in order to estimate the total uncertainty and to identify the major sources of uncertainty. Absorbed dose data from 115 treatment fractions are reported.

Results
The total absorbed dose with uncertainty is presented for 59 treatments with [177Lu]Lu-DOTATOC and 56 treatments with [177Lu]Lu-DOTATATE. For [177Lu]Lu-DOTATOC the mean and median specific absorbed dose (dose per injected activity) is 0.37 Gy/GBq and 0.38 Gy/GBq, respectively, while for [177Lu]Lu-DOTATATE the median and mean are 0.47 Gy/GBq and 0.46 Gy/GBq, respectively. The uncertainty of the procedure is estimated to be about 13% for a single treatment fraction, where the absorbed dose calculation is based on three SPECT/CT scans 1, 4 and 7 days post-injection, while it increases to about 19% if only a single SPECT/CT scan is performed 1 day post-injection.

Conclusions
The specific absorbed dose values obtained with the described procedure are comparable to those from other treatment sites for both [177Lu]Lu-DOTATOC and [177Lu]Lu-DOTATATE, but towards the lower end of the range of reported values. The estimated uncertainty is also comparable to that from other reports and judged acceptable for clinical and research use, thus proving the kidney dosimetry procedure a useful tool. The greatest reduction in uncertainty can be obtained by improved activity determination, partial volume correction and additional SPECT/CT scans.
Protocol for the RELATE trial: a feasibility and pilot randomised controlled trial of a low-intensity group intervention for young people in care with elevated posttraumatic stress symptoms
Authors: Hiller Rachel M. , Davis Rebecca S. , Devaney John , Halligan Sarah L. , Meiser-Stedman Richard , Smith Patrick , Stallard Paul , Kandiyali Rebecca , MacNeill Stephanie 
Published: November 2021

Abstract
Introduction
Young people in out-of-home care have often experienced trauma, such as direct maltreatment or witnessing violence. There is good evidence that rates of mental health difficulties are high in this group, including posttraumatic stress disorder (PTSD), a trauma-specific mental health outcome. There remains less evidence to guide how to effectively address elevated PTSD symptoms (PTSS) in these young people, particularly in ways that are feasible and scalable for stretched social-care and mental health services.

Methods and analysis
This protocol describes a feasibility study comprising a pilot two-arm randomised controlled trial (RCT). Participants (N = 50) will be randomised to either (a) a group-based trauma-focused programme (Teaching Recovery Techniques), delivered by mental health practitioners both online and in-person, or (b) care-as-usual. Primarily, the trial aims to explore the key feasibility and protocol acceptability questions, including rates of recruitment and retention, as well as the acceptability of the intervention (particularly the online delivery format) to participants and services. In addition, outcomes including PTSS (primary clinical outcome), depression and functioning will be assessed at baseline (pre-randomisation), post-intervention and at a 3-month follow-up.

Ethics and dissemination
Ethical approval has been received from the Health Research Authority (Wales REC1 Ref 20/WA/0100) and University, with further approval from the host trust and social care site. The results will inform the design of a definitive RCT. Dissemination will include peer-reviewed journal articles reporting the qualitative and quantitative results, as well as presentations at conferences and lay summaries.

Trial registration
ClinicalTrials.gov, NCT04467320. Registered on 13 July 2020.
Quantifying how urban landscape heterogeneity affects land surface temperature at multiple scales
Authors: Rahimi Ehsan , Barghjelveh Shahindokht , Dong Pinliang 
Published: November 2021

Abstract
Background
Landscape metrics have been widely applied to quantifying the relationship between land surface temperature and urban spatial patterns and have received acceptable verification from landscape ecologists but some studies have shown their inaccurate results. The objective of the study is to compare landscape metrics and texture-based measures as alternative indices in measuring urban heterogeneity effects on LST at multiple scales.

Results
The statistical results showed that the correlation between urban landscape heterogeneity and LST increased as the spatial extent (scale) of under-study landscapes increased. Overall, landscape metrics showed that the less fragmented, the more complex, larger, and the higher number of patches, the lower LST. The most significant relationship was seen between edge density (ED) and LST (r = − 0.47) at the sub-region scale. Texture measures showed a stronger relationship (R2 = 34.84% on average) with LST than landscape metrics (R2 = 15.33% on average) at all spatial scales, meaning that these measures had a greater ability to describe landscape heterogeneity than the landscape metrics.

Conclusion
This study suggests alternative measures for overcoming landscape metrics shortcomings in estimating the effects of landscape heterogeneity on LST variations and gives land managers and urban planners new insights into urban design.
Exploring the phyto- and physicochemical evaluation, fluorescence characteristics, and antioxidant activities of Acacia ferruginea Dc: an endangered medicinal plant
Authors: Jeevitha M. , Ravi Pavithra V. , Subramaniyam Vinodhini , Pichumani Moorthi , Sripathi Shubashini K. 
Published: November 2021

AbstractBackgroundHerbal plants are potent in curing various ailments of ancient times as they have comparatively lesser side effects. The demands for natural drugs, mostly from plant sources, are increasing over the past few decades. Because of their potent antioxidant activity, Acacia species are used to treat a variety of diseases. One of the speciesAcacia ferruginea, an endangered medicinal plant, is widely used in the traditional medicine system, and it is considered that standardization would be beneficial. The present study investigates the physicochemical parameters, preliminary phytochemical screening, trace metals by SEM–EDS, and fluorescence properties of various extracts (non-polar to polar) of leaf and bark parts. Standard spectrophotometric methods (UV–Vis, FT-IR, fluorescence spectroscopy) are employed to analyze the functional groups, and the DPPH and total antioxidant methods are used to assess antioxidant potential.ResultsThe ethyl acetate extract of leaves and ethanol extract of the bark are found to be the highest in yield, 16.32% and 2.54%. Results reveal that the total ash percentage and moisture content are of bark and the water-soluble ash of leaves is higher (10.3 ± 0.85, 7.6 ± 0.34, 3.22 ± 0.24%). The bark polar extract contained more macro-elements such as Na, K, Mg, Ca, S, and Cl. Phytochemical analysis reveals the polar extracts of leaves and bark show saponins, flavonoids, steroids, phenolic compounds, and non-polar extracts show mild positive. The total alkaloids, phenolics, and terpenoids (1.58 ± 0.08%; 0.56 ± 0.11; 0.75 ± 0.15) are found to be higher inA. ferruginealeaves. The FT-IR result shows the presence of alkanes, alkenes, aromatic compounds, aldehydes, phenolics and does not contain any toxic substances since there is no peak observed in the region between 2220 and 2260 cm−1. The in vitro antioxidant activity of the species demonstrated that both the leaf and bark parts have prominent antioxidant properties.ConclusionsThe results obtained from the preliminary standardization ofA. ferrugineaare very helpful in the determination of the quality and purity of the crude drug. The refurbished findings ofA. ferrugineaare promising, and further research is important to identify the bioactive compounds, thereby developing nutritional supplements and medications through therapeutic compound isolation.Graphical Abstract
Emergencies in obese patients: a narrative review
Authors: Di Giacinto Ida , Guarnera Martina , Esposito Clelia , Falcetta Stefano , Cortese Gerardo , Pascarella Giuseppe , Sorbello Massimiliano , Cataldo Rita 
Published: November 2021

AbstractObesity is associated to an increased risk of morbidity and mortality due to respiratory, cardiovascular, metabolic, and neoplastic diseases. The aim of this narrative review is to assess the physio-pathological characteristics of obese patients and how they influence the clinical approach during different emergency settings, including cardiopulmonary resuscitation. A literature search for published manuscripts regarding emergency and obesity across MEDLINE, EMBASE, and Cochrane Central was performed including records till January 1, 2021. Increasing incidence of obesity causes growth in emergency maneuvers dealing with airway management, vascular accesses, and drug treatment due to both pharmacokinetic and pharmacodynamic alterations. Furthermore, instrumental diagnostics and in/out-hospital transport may represent further pitfalls. Therefore, people with severe obesity may be seriously disadvantaged in emergency health care settings, and this condition is enhanced during the COVID-19 pandemic, when obesity was stated as one of the most frequent comorbidity. Emergency in critical obese patients turns out to be an intellectual, procedural, and technical challenge. Organization and anticipation based on the understanding of the physiopathology related to obesity are very important for the physician to be mentally and physically ready to face the associated issues.
Sleep as a Major Determinant for Mental Health Outcomes in Elite Australian Football League (AFL) Athletes
Authors: FACER-CHILDS ELISE R. , MASCARO LUIS , HOFFMAN DANIEL , MANSFIELD DARREN , DRUMMOND SEAN P. A. , RAJARATNAM SHANTHA M. W. 
Published: November 2021

ABSTRACT

Purpose
The link between mental health and sleep is well documented in the general population, with the majority of mental health disorders involving some type of sleep disturbance. There is, however, limited research investigating this relationship in elite athlete populations. The aim of this study was to identify whether sleep and mental health outcomes are associated in elite athletes and, if so, what measures of sleep were the most predictive of mental health outcomes.

Methods
A comprehensive assessment of sleep was conducted using both objective and subjective methods in 68 Australian Football League athletes (male; mean age, 23.3 ± 3.4 yr; median, 23; range, 18–32 yr). Rest-activity patterns were recorded using wrist actigraphy for an average of 13.8 ± 3.6 d (a total of 884 d of data). Subjective sleep data were collected using daily sleep diaries and validated questionnaires. Validated mental health questionnaires were used to assess depression, anxiety, and stress symptoms. Multiple linear regression modeling was used to investigate the relationship between sleep and mental health.

Results
Using a combination of sleep variables, poor sleep predicted 51% of the variation in clinical depression, 42% of the variation in stress, and 31% in clinical anxiety. Self-reported insomnia symptoms (using the Insomnia Severity Index) were the strongest predictors of poor mental health outcomes, followed by objective sleep monitoring via actigraphy. Sleep diary measures were the weakest predictors of mental health.

Conclusions
Our results present poor sleep as a major determinant of impaired mental health outcomes in a population that is constantly under pressure to perform at the highest level and may underreport mental health symptoms. These findings support the inclusion of sleep assessments as an initial screening tool as well as a core component of all routine health and rehabilitation programs.
The Motor Engram of Functional Connectivity Generated by Acute Whole-Body Dynamic Balance Training
Authors: Ueta Kenji , Mizuguchi Nobuaki , Sugiyama Takashi , Isaka Tadao , Otomo Satoshi 
Published: November 2021

ABSTRACT

Purpose
Whole-body dynamic balance is necessary for both athletic activities and activities of daily living. This study aimed to investigate the effect of acute dynamic balance training on neural networks.

Methods
We evaluated resting-state functional connectivity (rs-FC), white matter fiber density, fiber-bundle cross-section, and gray matter volume in 28 healthy young adults (14 women) before and after 30 min of slackline training using a randomized, counterbalanced crossover design.

Results
The rs-FC between the left lateral prefrontal cortex (PFC) and the foot area of the primary sensorimotor (SM1) cortex increased significantly after slackline training compared with that after a control condition involving ergometer-based aerobic exercise. In addition, changes in rs-FC between the left lateral PFC and the primary sensorimotor were correlated with performance changes after training (i.e., offline process) rather than online learning. We also observed a main effect of time between the hippocampus and the cingulate cortex, including the anterior areas, and between the bilateral lateral PFC. Although we observed no structural changes, fiber density in the commissural fiber pathway before the first balance assessment was correlated with initial balance capability.

Conclusions
Our findings demonstrate that acute whole-body dynamic balance training alters specific rs-FC, and that this change is associated with performance changes after training. In addition, rs-FC changes in cognitive regions were modulated by both acute dynamic balance training and aerobic exercise. These findings have the potential to influence various fields (e.g., sports neuroscience, neurorehabilitation) and may aid in the development of methods that can improve motor and cognitive performance.