bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of chicken-specific pro-angiogenic growth factors three-dimensional structures and analysis of their role in high molecular weight heparin-induced angiogenesis
Authors: Manjunathan Reji , Periyaswami Vijayalakshmi , Ragunathan Malathi 
Published: August 2021

Abstract
Background

- High-molecular weight heparin (HMWH), a molecule which is extensively in use as an anticoagulant shows concentration-dependent angiogenic and anti-angiogenic potential. Based on the concentration, HMWH can bind with both angiogenic and anti-angiogenic factors and exerts diverse effect. Our earlier data suggested that HMWH (15 kDa) can induce concentration-dependent neovascularization on chicken chorioallantoic membrane (CAM). The diffusion pattern of HMWH through various layers of CAM supports its internalized action with the various cellular components of angiogenesis. So far, no studies have reported the interactive potential of HMWH with various pro-angiogenic growth factors under physiological conditions. Hence, we aimed to find the transcription level interaction of HMWH with major pro-angiogenic growth factors. In connection to the research, for the first time, we validated the three-dimensional structures of chicken-specific pro-angiogenic growth factors such as FGF2, MMP2, MMP9, NOS3, VEGF A, and VEGF C to find the binding affinity of HMWH with the core-functional units of these growth factors.
Methods

- CAMs are incubated with 50, 100, and 150 µM concentration of HMWH. Changes in the transcription level of specified pro-angiogenic growth factors are analyzed by semi-PCR method. The functional aspects of these molecules are identified with zymogram and immunohistochemical approaches. Scanning electron microscopic technique is applied to find the morphological changes on CAM under HMWH incubation. Three-dimensional structure validation and molecular docking are performed using the SWISS-MODEL web server and AutoDock vina-PyRx software version 8.0.
Results

- HMWH can enhance the transcription level of major pro-angiogenic growth factors with a significant impact on FGF2 and MMP2 under 100 µM concentration. The in-silico analysis reveals that HMWH shows a higher binding affinity with FGF2 followed by MMP2, MMP9, NOS3, VEGF A, and VEGF C, respectively.
Conclusion

- The combined results from the experimental and in-silico analysis reveal that HMWH can interact with pro-angiogenic growth factors under micromolar concentration in physiological conditions while inducing angiogenesis. This observation further supports the therapeutic benefits of HMWH as an angiogenic factor under micromolar concentration.
A Novel Multi-Attribute Decision-Making Algorithm for Landfill Site Selection Based on Q-Rung Orthopair Probabilistic Hesitant Fuzzy Power Weight Muirhead Mean Operator
Authors: Ren Yaojun , Yuan Xiujiu , Lin Ruojing 
Published: August 2021

Abstract
BackgroundWith the rapid development of economy and the acceleration of urbanization, the garbage produced by urban residents also increases with the increase of population. In many big cities, the phenomenon of "garbage siege" has seriously affected the development of cities and the lives of residents. Sanitary landfill is an important way of municipal solid waste disposal. However, due to the restriction of social, environmental and economic conditions, landfill site selection has become a very challenging task. In addition, landfill site selection is full of uncertainty and complexity due to the lack of cognitive ability of decision-makers and the existence of uncertain information in the decision-making process.MethodsA novel multi-attribute decision making method based on q-rung orthopair probabilistic hesitant fuzzy power weight Muirhead mean (q-ROPHFPWMM) operator is proposed in this paper, which can solve the problem of landfill site selection well. This method uses probability to represent the hesitance of decision maker and retains decision information more comprehensively. The negative effect of abnormal data on the decision result is eliminated by using the power average operator. Muirhead mean operator is used to describe the correlation between attributes. ResultsAn example of landfill site selection is given to verify the effectiveness of the proposed method, and the advantages of the proposed method are illustrated by parameter analysis and comparative analysis. ConclusionThe q-ROPHFPWMM operator can describe the correlation between any evaluation factors and effectively reduce the impact of unreasonable evaluation information given by decision makers on the results. In addition, this method has a wider space for information expression, gives the decision maker a great degree of freedom in decision-making.
Influence of Thermal Activation and Silica Modulus on the Properties of Clayey-Lateritic Based Geopolymer Binders Cured at Room Temperature
Authors: METEKONG Jordan Valdès SONTIA , Kaze Cyriaque Rodrigue , Adesina Adeyemi , Nemaleu Juvenal Giogetti Deutou , Djobo Jean Noel Yankwa , Lemougna Patrick Ninla , Alomayri Thamer , Kamseu Elie , Melo Uphie Chinje , Tatietse Thomas Tamo 
Published: August 2021

Abstract
The growing demand and use of geopolymer binders have been of high interest in recent times due to their sustainability and economic benefits. However, locally available materials must be used in the production of geopolymer binders in order to maximize their benefits. In this study, a laterite soil which is a locally available material in many parts of the world was used as the aluminosilicate precursor. In order to produce the geopolymer binders, the laterite soil was activated thermally through calcination and the resulting calcined laterite was activated with an alkali activator composed of sodium hydroxide (NaOH) and sodium silicate (Na2SiO3). The influence of calcination temperature and the concentration of NaOH on the properties of the developed geopolymers was investigated. Calcination temperature used ranged between 550 °C to 750 °C and the NaOH concentrations used were 8 M and 10 M. The results from this study indicate that some locally available laterite soils can be utilized in the production of geopolymer binders if they undergo calcination. Increasing the calcination temperature from 550 °C to 750 °C resulted in the transformation of phases and an increase in the reactivity of the laterites, resulting in material with improved properties. The use of laterite calcined at 750 °C and activated with 8 M NaOH solution resulted to an increase in the 28 days compressive strength by 35.3 MPa compared to when laterite calcined at 550 °C was used. Increasing the concentration of the NaOH solution was also found to yield higher material performance. Microstructural investigations were also carried out to confirm the macrostructural properties.
Commercialization of Teff production in West North Ethiopia
Authors: Mossie Habtamu , Abate Dubale , Kasse Eden 
Published: August 2021

Abstract
Background: Teff is only cereal crop Ethiopia’s in terms of production, acreage, and the number of farm holdings. It is one of the staples crops produced in the study area. However, the farm productivity, commercialization and level of intensity per hectare is low compared to the other cereals , Despite, smallholder farmers are not enough to participate in the teff market so the commercialization level is very low due to different factors. so, the study aimed to analyze determinants of smallholder farmer’s teff commercialization in west north, Ethiopia.Methods; A three-stage sampling procedure was used to take the sample respondents, 190 smallholder teff producers were selected to collect primary data through semi-structures questionnaires. Combinations of data analysis methods such as descriptive statistics and econometrics model (double hurdle) were used.Results: The average level of teff commercialization index of sampled Teff producers in the study area was 11.97%. The model result showed that the variables like age, gender, education, land size, variety, Teff market experience, distance to the nearest market, off-farm income, and credit were significantly affecting the market orientation of Teff production. Therefore, improving households’ market access, extension service, market orientation, education, and productivity of land (best agronomic practices) would enhance the commercialization level of smallholder Teff producers.
Design and Study of In Silico Binding Dynamics of Certain Isoxazole Bearing Leads Against Aβ-42 and BACE-1 Loop in Protein Fibrillation
Authors: Mishra Puja , Basak Souvik , Mukherjee Arup , Basu Anindya 
Published: August 2021

Aims:
Design isoxazole bearing leads as dual inhibitors against Amyloid β and BACE-1 loop
in protein fibrillation.

Background:
Protein fibrillation is one of the key reasons for several diseases, namely Alzheimer’s, Parkinson’s,
and many others. One of the key strategies of preventing protein fibrillation is destabilizing the
protein fibrils themselves or inhibiting the amyloid fibril-forming pathway in the initial stage.

Introduction:
Attempts have been taken to design newer leads to inhibit protein fibrillation by targeting
the β-amyloidogenesis pathway in the brain. To exploit interfenestration between Amyloid β -42 protein
and BACE-1 (β-site amyloid precursor protein cleaving enzyme) for amyloidogenesis, studies are undertaken
to design dual inhibitors against the same.

Method:
In vitro binding interactions were found using docking, de novo ligand design, and MD simulation
study.

Results:
Three compounds bearing an isoxazole heterocyclic nucleus were designed which could successfully
bind to the hydrophobic raft and salt bridge residues Asp 23-Lys-26 of Amyloid β, destabilizing the
growing fibril. Additionally, one of our candidate compounds exhibited force of interaction with Thr232
at the S3 pocket of BACE-1, interacted with key residue Asp228, Tyr71, and Thr72 of the β-hairpin flap
and hydrogen bonding with Gly11 at loop 10s.

Conclusion:
Protein flexibility dynamics of the Aβ-42 protein revealed that there is a considerable conformational
change of the same with or without ligand binding. The lower RMSF of the bound region and
reprogramming residual contacts within the Aβ-42 protein suggested successful binding of the ligand with
the protein, lowering the access for further β-β dimerization.
Methylene Blue not Contraindicated in Treating Hemodynamic Instability in Pediatric and Neonate Patients
Authors: Gonçalves-Ferri Walusa A. , Albuquerque Agnes A.S. , Evora Patricia Martinez , Evora Paulo R.B. 
Published: August 2021

:
The present review was carried out to describe publications on the use of methylene
blue (MB) in pediatrics and neonatology, discussing dose, infusion rate, action characteristics, and
possible benefits for a pediatric patient group. The research was performed on the data sources
PubMed, BioMed Central, and Embase (updated on Aug 31, 2020) by two independent investigators.
The selected articles included human studies that evaluated MB use in pediatric or neonatal patients
with vasoplegia due to any cause, regardless of the applied methodology. The MB use and 0
to 18-years-old patients with vasodilatory shock were the adopted criteria. Exclusion criteria were
the use of MB in patients without vasoplegia and patients ≥ 18-years-old. The primary endpoint
was the increase in mean arterial pressure (MAP). Side effects and dose were also evaluated.
Eleven studies were found, of which 10 were case reports, and 1 was a randomized clinical study.
Only two of these studies were with neonatal patients (less than 28 days-old), reporting a small
number of cases (1 and 6). All studies described the positive action of MB on MAP, allowing the
decrease of vasoactive amines in several of them. No severe side effects or death related to the use
of the medication were reported. The maximum dose used was 2 mg/kg, but there was no consensus
on the infusion rate and drug administration timing. Finally, no theoretical or experimental basis
sustains the decision to avoid MB in children claiming it can cause pulmonary hypertension.
The same goes for the concern of a possible deleterious effect on inflammatory distress syndrome.
Electrochemical Sensing of Dextrose and Photocatalytic Activities by Nickel Ferrite Nanoparticles Synthesized by Probe Sonication Method
Authors: Alam Mir Waqas 
Published: August 2021

Background:
Electrochemical sensing has been widely used as a glucose sensor.

Methods:
In the present work, we have investigated the application of nickel ferrite (NiFe2O4) nanoparticles
(NFNPs) for dextrose sensing applications. NFNPS were synthesized by using a simple and
low-cost probe sonication method (PSM). The structural, optical and electrochemical properties of
prepared nanoparticles were measured by using XRD, FTIR and DRS techniques.

Results:
The PXRD pattern confirmed the formation of inverse spinel structure for NFNPs with a
face-cente red cubic (FCC) structure. The diffuse reflectance spectral studies revealed the energy
band gap of NFNPs as deduced to be 2.50 eV by using Kubelka-Munk function. The efficiencies in
the photocatalytic degradation of Acid Red 88 (AR-88) and Acid Blue 88 (AB-88) dyes were found
to be better with 93.45% and 84.45%, respectively. The EIS parameters corroborated the significant
reduction in the charge transfer resistance of NFNPs electrode. The sensing of dextrose in acidic
solution by using NFNP through carbon paste electrode was successful.

Conclusion:
The obtained sensing and photocatalytic results revealed that the synthesized NFNPs
could be a better electrode material for the detection of dextrose with high electrode reversibility, in
addition to its excellent photocatalytic characteristics towards the dye degradation.
Efficacy and Safety of Mesenchymal Stem Cell Therapy in Patients with Acute Myocardial Infarction: A Systematic Review and Meta-analysis of Randomized Controlled Trials
Authors: Zhang Run-feng , Yu Jiang , Mao Yi-li , Zhang Heng 
Published: August 2021

Background and Objectives:The adjuvant treatment of stem cell therapy for acute myocardial infarction (AMI) has been studied in multiple clinical trials, but many questions remain to be addressed, such as efficacy, safety, identification of the optimal cell type, tractable route of delivery, transplant dosage, and transplant timing. The current meta-analysis aimed to explore the issues of mesenchymal stem cells (MSCs) transplantation in patients with AMI based on published randomized controlled trials (RCTs) and guide the design of subsequent clinical trials of MSCs therapy for AMI.Methods:The Cochrane Library, PubMed, EMBASE databases were searched for relevant clinical trials from January 1, 2000, to January 23, 2021. Results from RCTs involving MSCs transplantation for the treatment of AMI were identified. According to the Cochrane systematic review method, the literature quality, including studies, was evaluated and valid data was extracted. Rev- Man 5.3 and Stata 15.1 software were used for Meta-analysis.Results:After literature search and detailed evaluation, 9 randomized controlled trials enrolling 460 patients were included in the quantitative analysis. Pooled analyses indicated that MSCs therapy was associated with a significantly greater improvement in overall left ventricular ejection fraction (LVEF), and the effect was maintained for up to 24 months. No significant difference in favor of MSCs treatment in left ventricular (LV) volume and in the risk of rehospitalization as a result of heart failure (HF) was noted, compared with the controls. For transplantation dose, the LVEF of patients accepting a MSCs dose of 107-108 cells was significantly increased by 2.62% (95% CI 1.54 to 3.70; P < 0.00001; I2 =0%), but this increase was insignificant in the subgroup that accepted a MSCs dose of < 107 cells (1.65% in LVEF, 95% CI, 0.03 to 3.27; P =0.05; I2 =75%) or >108 cells (4.65% in LVEF, 95% CI, -4.55 to 13.48; P =0.32; I2 =95%), compared with the controls. For transplantation timing, a significant improvement of LVEF of 3.18% was achieved in the group of patients accepting a MSCs infusion within 2 to 14 days after percutaneous coronary intervention (PCI) (95% CI, 2.89 to 3.47; P <0.00001; I2 = 0). There was no association between MSCs therapy and major adverse events.Conclusion:Results from our systematic review suggest that MSCs therapy for patients with AMI appears to be safe and might induce a significant increase in LVEF with a limited impact on LV volume and rehospitalization caused by HF. The effect was maintained for up to 24 months. MSCs dose of 107-108 cells was more likely to achieve better clinical endpoints than <107 or >108 cells. The optimal time window for cell transplantation might be within 2-14 days after PCI. This meta-analysis was registered with PROSPERO, number CRD 42021241104.
Rolipram Rescues Memory Consolidation Deficits Caused by Sleep Deprivation:
Implication of the cAMP/PKA and cAMP/Epac Pathways
Authors: Maher Ahmed , El Sayed Nesrine , Nafea Heba , Gad Mohamed 
Published: August 2021

Background:
Over the last few years, the number of people suffering from sleeping disorders
has increased significantly despite negative effects on cognition and an association with
brain inflammation.

Objectives:
We assessed memory deficits caused by Sleep Deprivation (SD) to determine the therapeutic
effect of phosphodiesterase 4 (PDE4) inhibitors on SD-induced memory deficits and to investigate
whether the modulation of memory deficits by PDE4 inhibitors is mediated by a protein
kinase A (PKA)-independent pathway in conjunction with a PKA-dependent pathway.

Methods:
Adult male mice were divided into four groups. Three SD groups were deprived of
Rapid Eye Movement (REM) sleep for 12 h a day for six consecutive days. They were tested daily
in the Morris water maze to evaluate learning and memory. One of the SD groups was injected
with a PDE4 inhibitor, rolipram (1 mg/kg ip), whereas another had rolipram co-administered with
chlorogenic acid (CHA, 20 mg/kg ip), an inhibitor of PKA. After 6 days, the mice were sacrificed,
and the hippocampi were evaluated for cyclic AMP (cAMP) and nuclear factor Nrf-2 levels. The
hippocampal expression of PKA, phosphorylated cAMP Response Element-Binding Protein (CREB),
and phosphorylated glycogen synthase 3β (Ser389) were also evaluated.

Results:
SD caused a significant decrease in cAMP levels in the brain and had a detrimental effect
on learning and memory. The administration of rolipram or rolipram+CHA resulted in an improvement
in cognitive function.

Conclusion:
The present study provides evidence that restoration of memory with PDE4 inhibitors
occurs through a dual mechanism involving the PKA and Epac pathways.
Degradation of the O-phenylphenol Fungicide in Water by Unconventional CeO2-WO3 Photocatalysts
Authors: Fiorenza Roberto , Balsamo Stefano Andrea , Scirè Salvatore 
Published: August 2021

Background:
Water pollution due to emerging contaminants such as pesticides, pharmaceutics and/or plasticizers, is a serious environmental problem strictly connected to the safety of human and ecosystem life. For this reason, the development of high-performing (photo)catalysts for water purification is crucial.

Objective:
In recent years, the synergistic effects in Advanced Oxidation Processes (AOPs) can perform better strategies to remove recalcitrant contaminants from water. In this contest, the (photo)catalytic activity of CeO2-WO2materials for the degradation of the orto-phenylphenol fungicide comparing the photocatalytic, the Fenton and the photo-Fenton-like processes, has been examined.

Methods:
The samples were synthetized through deposition-precipitation mediated with the hexamethylenetetramine (HMTA) surfactant. The chemico-physical properties of the materials were examined by Raman, UV-Vis Diffuse Reflectance (Uv-vis DRS) and X-Ray photoelectron (XPS) spectroscopies, N2 adsorption-desorption measurements and transmission electron microscopy (TEM). The (photo)catalytic measurements were made through a home-made photoreactor irradiated by a solar lamp. The degradation of the fungicide was measured by UV-vis spectroscopy.

Results :
An efficient heterojunction was formed between the CeO2 and the WO2 oxides, which provided a good degradation percentage of the pesticide (65%) employing the solar photo-Fenton-like reaction that was the best performing process among the three investigated AOPs. The addition of WO3on CeO2 facilitated the ionic exchange between the Ce and the W ions, boosting the redox properties of cerium oxide.

Conclusions:
The strong interaction between CeO2 and WO3 and the peculiar properties of this unconventional composite pave the way to its use as a promising material for water depollution.
Development and Validation of an Arterial Pressure-Based Cardiac Output Algorithm Using a Convolutional Neural Network: Retrospective Study Based on Prospective Registry Data
Authors: Yang Hyun-Lim , Jung Chul-Woo , Yang Seong Mi , Kim Min-Soo , Shim Sungho , Lee Kook Hyun , Lee Hyung-Chul 
Published: August 2021

Background
Arterial pressure-based cardiac output (APCO) is a less invasive method for estimating cardiac output without concerns about complications from the pulmonary artery catheter (PAC). However, inaccuracies of currently available APCO devices have been reported. Improvements to the algorithm by researchers are impossible, as only a subset of the algorithm has been released.

Objective
In this study, an open-source algorithm was developed and validated using a convolutional neural network and a transfer learning technique.

Methods
A retrospective study was performed using data from a prospective cohort registry of intraoperative bio-signal data from a university hospital. The convolutional neural network model was trained using the arterial pressure waveform as input and the stroke volume (SV) value as the output. The model parameters were pretrained using the SV values from a commercial APCO device (Vigileo or EV1000 with the FloTrac algorithm) and adjusted with a transfer learning technique using SV values from the PAC. The performance of the model was evaluated using absolute error for the PAC on the testing dataset from separate periods. Finally, we compared the performance of the deep learning model and the FloTrac with the SV values from the PAC.

Results
A total of 2057 surgical cases (1958 training and 99 testing cases) were used in the registry. In the deep learning model, the absolute errors of SV were 14.5 (SD 13.4) mL (10.2 [SD 8.4] mL in cardiac surgery and 17.4 [SD 15.3] mL in liver transplantation). Compared with FloTrac, the absolute errors of the deep learning model were significantly smaller (16.5 [SD 15.4] and 18.3 [SD 15.1], P<.001).

Conclusions
The deep learning–based APCO algorithm showed better performance than the commercial APCO device. Further improvement of the algorithm developed in this study may be helpful for estimating cardiac output accurately in clinical practice and optimizing high-risk patient care.