bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decreased Expression of KPNA2 in Hepatocellular Carcinoma Infers Favorable Prognosis and High Immune Infiltrating Level
Authors: zhu kangming , zhang yvndi , yvan hui , li jing 
Published: December 2021

Abstract
BackgroundLiver hepatocellular carcinoma (LIHC) is an important pathological type of liver cancer. The immune infiltration of the tumor microenvironment is negatively correlated with the overall survival rate of LIHC. At present , the role and molecular mechanism of KPNA2 in LIHC have not been elucidated, and the prognostic correlation between the two and the immune infiltration of LIHC are still unclear. Our study evaluated the role of KPNA2 in LIHC through TCGA data.MethodGene expression profiling interactive analysis (GEPIA) is used to analyze the expression of KPNA2 in LIHC. We evaluated the impact of KPNA2 on the survival of LIHC patients through the survival module. Then, We downloaded the LIHC data set from TCGA. Logistic regression was used to analyze the correlation between clinical information and KPNA2 expression. Cox regression analysis was used to analyze the clinicopathological characteristics related to the overall survival rate of TCGA patients. In addition, we used the "correlation" modules of CIBERSORT and GEPIA to explore the correlation between KPNA2 and cancer immune infiltrate. Western blotting was used to detect the expression of KPNA2.ResultUsing logistic regression for univariate analysis, increased KPNA2 expression was significantly correlated with pathological stage, tumor status, and lymph node status. In addition, multivariate analysis showed that down-regulation of KPNA2 expression, negative pathological stage and distant metastasis are independent prognostic factors for good prognosis. Specifically, CIBERSORT analysis was used to establish a negative correlation between the up-regulated expression of KPNA2 and the level of immune infiltration of B cells, NK cells, mast cells, and T cells. In addition, we confirmed this in the "Association" module of GEPIA. The expression of KPNA2 in LIHC tissues was significantly lower than that in adjacent normal tissues by western blotting.ConclusionThe down-regulation of KPNA2 expression is associated with a good prognosis and an increase in the proportion of immune cells in LIHC. These conclusions indicate that KPNA2 is related to the level of immune infiltration of LIHC and can be used as a potential prognostic biomarker of LIHC and a potential target for clinical tumor treatment.
Effects of Sodium-Glucose Cotransporter-2 Inhibitors on Cardiac Structural
and Electrical Remodeling: From Myocardial Cytology to Cardiodiabetology
Authors: Marketou Maria , Kontaraki Joanna , Maragkoudakis Spyros , Danelatos Christos , Papadaki Sofia , Zervakis Stelios , Plevritaki Anthoula , Vardas, Panos , Parthenakis Fragiskos , Kochiadakis George 
Published: December 2021

Abstract:
Sodium-glucose cotransporter 2 inhibitors (SGLT2i) have changed the clinical landscape
of diabetes mellitus (DM) therapy through their favourable effects on cardiovascular outcomes.
Notably, the use of SGLT2i has been linked to cardiovascular benefits regardless of DM status,
while their pleiotropic actions remain to be fully elucidated. What we do know is that SGLT2i
exert beneficial effects even at the level of the myocardial cell and that these are linked to an improvement
in the energy substrate, resulting in less inflammation and fibrosis. SGLT2i ameliorates
myocardial extracellular matrix remodeling, cardiomyocyte stiffness and concentric hypertrophy,
achieving beneficial remodeling of the left ventricle with significant implications for the pathogenesis
and outcome of heart failure. Most studies show a significant improvement in markers of diastolic
dysfunction along with a reduction in left ventricular hypertrophy. In addition to these effects,
there is electrophysiological remodeling, which explains initial data suggesting that SGLT2i have
an antiarrhythmic action against both atrial and ventricular arrhythmias. However, future studies
need to clarify not only the exact mechanisms of this beneficial functional, structural, and electrophysiological
cardiac remodeling but also its magnitude to determine whether this is a class or a
drug effect.
A Real-world Evidence-based Management of HIV by Differential Duration
HAART Treatment and its Association with Incidence of Oral Lesions
Authors: Li Cheng-Wen , Shu Wen , Du Fei , Bai Jin-Song , Yin Ling-Yun , Duan Kai-Wen 
Published: December 2021

Background:
The efficacy of highly active antiretroviral therapy (HAART) therapy can be estimated by immunological response and the incidence of opportunistic infections.

Objective:
To evaluate the effectiveness of different durations of HAART in terms of immunological response markers (CD4 count and CD4/CD8 ratio) along with disease progression markers (incidence of oral lesions) in Chinese patients with HIV.

Methods:
This single-center, retrospective, real-world study included patients with HIV, grouped into treatment group and treatment-naïve group of which the former was further divided into (6, 12, and 18 months) based on the treatment duration. The CD4 and CD8 cell counts were analyzed by the FACSCalibur flow cytometry. Kruskal-Wallis test was applied to determine the outcome of different duration of HAART. Oral examination was carried out according to the WHO type IV examination

Results:
In 246 patients with HIV, CD4 counts increased significantly post-HAART compared with pre-HAART in all three treatment groups (P<.001), while CD8 count decreased significantly (P<.05) in all three treated group. A significant association of HAART with CD4/CD8 ratio was observed (P<.001). A significant increase in CD4 count was observed between 12-month and 18-month treatment group (P<.05). The occurrence of oral lesions reduced significantly in the treatment group.

Conclusion:
We observed a better response of HAART regimen with 18 months of duration than 12-months and 6-months therapies and reduction in oral lesions.
Molecular Docking of the Terpenes in Gorgonian Corals to COX-2 and
iNOS Enzymes as Anti-Inflammatory
Authors: Kelutur Faruk Jayanto , Saptarini Nyi Mekar , Mustarichie Resmi , Kurnia Dikdik 
Published: December 2021

Background:
Because the inflammatory pathway is triggered by the enzymes cyclooxygenase-
2 (COX-2) and inducible nitric oxide synthase (iNOS), inhibitors, such as nonsteroidal anti-inflammatory
drugs (NSAIDs), are needed, although these have side effects. Therefore, the discovery and development
of natural medicine as a lead compound are needed. The gorgonian corals have been reported to contain
cyclic diterpenes with anti-inflammatory activities. The specific anti-inflammatory inhibitor potential has
not been reported regarding these secondary metabolites, whether in COX-2 or iNOS. Thus, the in silico
method is the right alternative.

Objective:
This study aimed to determine the potency of fifteen terpenes of the various gorgonian corals
to COX-2 and iNOS enzymes as an anti-inflammatory.

Methods:
Molecular docking was performed using ChemDraw Ultra 12.0, Chem3D Pro 12.0, Biovia
Discovery Studio 2016 Client®, Autodock Tools 4.2, prediction pharmacokinetics (Pre-ADMET), and
oral administration (Lipinski rule of five).

Results:
Potential terpenes based on ΔG (kcal/mol) and Ki (nM) to COX-2 were gyrosanol B (-10,32;
27,15), gyrosanol A (-10,20; 33,57), echinolabdane A (-9,81; 64,76). Only nine terpenes were specific to
COX-2 active sites, while for iNOS were palmonine F (-7.76; 2070), briarenol C (-7.55; 2910), and all
test compounds binding to the iNOS active sites. Pre-ADMET prediction obtained that HIA was very
excellent (70–100%), Caco-2 had moderate permeability (4–70 nm sec-1), and PPB had strong binding (>
90%). Eight terpenes qualified for the Lipinski rule of five.

Conclusion:
iNOS was a specific target for terpenes based on the free energy of binding (ΔG).
Fetal Cardiac Function: Myocardial Performance Index
Authors: Oliveira Mariana , Dias Joana Portela , Guedes-Martins Luís 
Published: December 2021

Abstract:
The Myocardial Performance Index (MPI) or Tei index, presented by Tei in 1995, is the
ratio of the sum of the duration of the isovolumetric contraction time (ICT) and isovolumetric relaxation
time (IRT) to the duration of the ejection time (ET). The Modified Myocardial Performance
Index (Mod-MPI), proposed in 2005, is considered a reliable and useful tool in the study of fetal
heart function in several conditions, such as growth restriction, twin-twin transfusion syndrome,
maternal diabetes, preeclampsia, intrahepatic cholestasis of pregnancy, and adverse perinatal outcomes.
Nevertheless, clinical translation is currently limited by poorly standardised methodology
as variations in the technique, machine settings, caliper placement, and specific training required
can result in significantly different MPI values. This review aims to provide a survey of the relevant
literature on MPI, present a strict methodology and technical considerations, and propose future
research.
ANALISIS STRATEGI PEMASARAN PRODUK ASURANSI JIWA MENGGUNAKAN METODE SWOT (STUDI PADA PT.TAKAFUL KELUARGA CABANG BANDA ACEH)
Authors: Nazila Tasya , Arifin Muhammad , Amanatillah Dara 
Published: December 2021

Abstrak
Produk asuransi jiwa merupakan produk yang mendominasi pada perusahaan asuransi PT. Takaful Keluarga Cabang Banda Aceh berdasarkan peningkatan nasabah pada setiap tahunnya, akan tetapi pada empat tahun terakhir terjadinya penurunan pertambahan jumlah nasabah dari tahun sebelumnya, sehingga diperlukan strategi pemasaran seperti analisis SWOT untuk meningkatkan kembali nasabah pada produk asuransi jiwa. Penelitian ini bertujuan untuk mengetahui analisis SWOT dalam strategi pemasaran pembiayaan produk asuransi jiwa pada PT. Takaful Keluarga Cabang Banda Aceh. Penelitian ini menggunakan metode penelitian kualitatif dengan teknik pengumpulan data berupa wawancara terstruktur. Hasil penelitian menunjukkan bahwa strategi pemasaran produk asuransi jiwa pada PT. Takaful Keluarga Cabang Banda Aceh menggunakan bauran pemasaran 4P yaitu product, price, place, dan promotion. Berdasarkan hasil analisis SWOT diketahui bahwa nilai skor IFAS adalah 2,39 dan nilai skor EFAS adalah 2,95. Artinya PT. Takaful Keluarga Cabang Banda Aceh dapat memanfaatkan peluang dan ancaman yang ada untuk meningkatkan pertumbuhan dan kemajuan secara maksimal.
Pengaruh Kualitas Pelayanan dan Kepercayaan Terhadap Kepuasan Nasabah Bank Rakyat Indonesia Syariah (Studi Pada Bank Rakyat Indonesia Syariah Cabang Darussalam Banda Aceh)
Authors: Vabiola Ghantynireta Bunga , Inayatillah Inayatillah , Evriyenni Evriyenni 
Published: December 2021

This study aims to determine the effect of service quality and trust on customer satisfaction at Bank Rakyat Indonesia Syariah Darussalam branch, Banda Aceh. This study uses a quantitative approach using multiple regression analysis. The sampling technique in this study was random sampling. Data collection techniques in this study by distributing questionnaires to 100 respondents. The results of this study indicate; (1) the service quality variable has no effect on customer satisfaction, (2) the trust variable has a positive and significant effect on customer satisfaction, and (3) together service quality and trust have an effect on customer satisfaction
The Role of Witness as Evidence in Divorce Cases at the Banda Aceh Syar’iyah Court
Authors: Devy Soraya , Bahri Syamsul , Ariga Selamat , Aslam Ahmad Muhammad , Buchary Budiman Mumtazinur , Sri Wahyuni Yenny 
Published: December 2021

This study examines the role of witnesses as evidence in a divorce case at the Banda Aceh Syari’iyah Court. This study aims to determine the legal basis of witnesses as evidence, the background of the witnesses who were submitted due to disputes or syiqaqand the position of female witnesses in divorce cases. This study uses empirical legal research or sociological law, which is a study whose object is legal phenomena using sociological theories. The legal phenomenon in question is the application of law at the Banda Aceh Syar’iyah Court, particularly regarding witness evidence in divorce cases. Data collection techniques were carried out by means of in-depth interviews with judges and literature studies related to the existence of witnesses and judges’ decisions as primary data related to witnesses as evidence. This study concludes that witnesses as evidence are based on the Qur’an and hadithand the applicable laws and regulations. Witnesses have existence as evidence used by judges as a consideration in deciding cases. In the case of a divorce caused by a dispute, the witness evidence comes from the close family background of the husband or wife. Close family who really know, see and hear the events that occur in their household. In civil procedural law for divorce cases at the Syar'iyah Court, women have the same and equal position as men in giving testimony. The testimony of close families and the sociological equality of women and men is a legal fact that must be appreciated to achieve justice for all parties.
Parenting Skills According to The Islamic Perspective Towards Family Well-Being
Authors: Zur Raffar Izzah Nur Aida , Hanin Hamjah Salasiah , Hasan Ahmad Dzaky , Nik Dahlan Nang Naemah 
Published: December 2021

Parents are leaders of the family who should play the role of building family well-being. Family well-being very much depends on the way of nurturing or raising and managing the family. In order to build a prosperous or happy family, the first and foremost matter which needs to be emphasised is parenting knowledge. Neglecting this parenting aspect will result in parents’ failure to effectively manage the family toward well-being, even causing suffering to children and is associated with bad behaviour such as committing murder, physical or sexual abuse, and neglecting their education. Hence, this research is conducted to study parenting skills according to the Islamic perspective, by using content analysis method. Research findings based on the views of Muslim scholars conclude that in the Islamic perspective, the parenting skills which parents need to master to become excellent parents are parenting knowledge, maintaining their relationship with Allah SWT, relationship with their children and relationship with fellow human beings, encompassing spiritual, mental, emotional, physical and social aspects. The implications of this research can help parents know the basic parenting skills according to the Islamic perspective, to build a prosperous family. This research also contributes knowledge to the authorities responsible for managing family development so that knowledge, skills and programs relating to parenting can be disseminated and practised in Muslim family life.
Optimasi Analisis Melting Curve untuk Skrining Cepat dan Sensitif Mutasi V1016G pada Aedes aegypti Resisten Sintetik Piretroid dengan Reaksi Rantai Polimerase Spesifik Alel
Authors: Widiastuti Dyah , Agustiningsih Agustiningsih , Ratna Sari Ihda Zuyina , Ramadhani Tri 
Published: December 2021

Detection of V1016G mutation is important for identifying the mechanism of synthetic pyrethroid resistance in Aedes aegypti population. The previous method has described an allele specific polymerase chain reaction (AS-PCR) using conventional PCR to detect the mutation. Although the method has great differentiating power and reproducibility, faster and more sensitive genotyping method is essential to accurately detect the mutation. This study evaluate the used of SYBR® Green real-time PCR and melting curve analysis (MCA) to identify the V1016G mutation. The collection of homozygous 1016G, heterozygous, and wild type (1016 V) mosquitoes DNA genome was extracted using genomic DNA mini kit. The SsoAdvanced™ Universal SYBR® Green Supermix was used to identify alleles by real-time PCR followed melting curve analysis of the amplicons. Melting curve analysis produced reproducible results for the loci tested. The melting temperature was reached at 78.5 oC for homozygous 1016G mosquito and at 86 oC for wild type mosquito. Meanwhile, the heterozigous mosquito revealed two peaks of melting temperature at both 78.5 oC and 86 oC. These easily interpretable and distinguishable melting curve results were consistent with AS-PCR results obtained for the same alleles. The described MCA application for screening V1016G mutation is fast and widely accessible also could be implemented under field conditions
CICLO DE SEMINÁRIOS EM QUÍMICA DOS DIVERSOS SABERES – UESB: UM RELATO DE EXPERIÊNCIA DURANTE A PANDEMIA
Authors: Silva Robson Almeida , Brito Mariele Moraes , Oliveira Geisa Sales , Sampaio Neiane Oliveira , Santos Amanda Pereira , Gonzaga Fabiany Cruz 
Published: December 2021

Introdução: A pandemia de COVID-19 declarada em março de 2020, fez com que o mundo inteiro voltasse a atenção ao contágio da nova doença. Universidades e demais meios acadêmicos tiveram que optar por alternativas para o prosseguimento das atividades, logo, diante da impossibilidade do projeto “I Ciclo de Seminários de Química dos Diversos Saberes” ocorrer de forma presencial, foi optado por alternativas remotas. Objetivos: Dilvugar diversas palestras voltadas a diferentes áreas da química e sua contribuição na ciência e no desenvolvimento social. Metodologia: Trata-se de um relato de experiência de ações desenvolvidas no Projeto de extensão durante a pandemia da SARS-CoV-2 pelos membros do Diretório Acadêmico dos Cursos de Química. Resultados: A participação da comunidade externa foi significativa na execusão do projeto de extensão. O projeto ocorreu com uma intensiva participação de discentes de ensino médio e ensino superior. O projeto se deu de forma interdisciplinar agregando participantes de diversas áreas do conhecimento tendo como majoritariedade as grandes áreas das Ciências Exatas e da Terra. Conclusão: Portanto, o projeto de extensão proporcionou uma integração, além dos muros da universidade, promovendo a integração da comunidade interna e externa e a ajudando-as compreender e promover colaborações científicas.
hyperkeratotic uremic stomatitis revealing decompensation of chronic kidney disease
Authors: moussaoui eya , salha wahbi ben , Sriha Baderedine , oualha lamia , douki nabiha 
Published: December 2021

Uremic stomatitis is a rare manifestation associated with longstanding
uremia in chronic renal failure patients. Its evolution is
favorable with earlier institution of renal replacement therapy. We
report a case of hyperkeratotic uremic stomatitis that revealed
decompensation of chronic kidney disease.
Five-year Randomized Clinical Trial on the Performance of Two Etch-and-rinse Adhesives in Noncarious Cervical Lesions
Authors: Matos TP , Hanzen TA , Almeida R , Tardem C , Bandeca MC , Barceleiro MO , Loguercio AD , Reis A 
Published: December 2021

SUMMARY

Objectives
To evaluate the 5-year clinical performance of two-step etch-and-rinse adhesives in noncarious cervical lesions (NCCL).

Methods and Materials
The sample comprised 35 adults with at least two similar-sized NCCL. Seventy restorations were placed, according to one of the following groups: Adper Single Bond 2 (SB) and Ambar (AM). The restorations were placed incrementally using a resin composite (Opallis). The restorations were evaluated at baseline and after 6 and 18 months and 5 years using some items of the FDI criteria. The differences in the ratings of the two materials after 6 months, 18 months, and 5 years were performed with Friedman repeated measures ANOVA by rank and McNemar test for significance in each pair (α=0.05).

Results
Five patients did not attend the 60-month recall. No significant differences were observed between the materials for any criteria evaluated. Twenty-one restorations failed (12 for SB and 9 for AM) after 60 months. Thus, the retention rate for SB at 60 months were 55.6% for SB and 71% for AM (p=0.32). After 60 months, 12 restorations (6 for SB and 6 AM) showed some loss of marginal adaptation (p=1.0). Slight marginal discoloration was observed in 10 restorations (6 for SB and 4 AM; p=0.91). Five restorations (2 for SB and 3 for AM) showed recurrences of caries (p=1.0).

Conclusions
Both two-step etch-and-rinse adhesives—Adper Single Bond 2, a polyalkenoic acid-containing adhesive, and Ambar, a 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-containing adhesive—showed acceptable clinical performance after 60 months.
Evaluation of Novel Plant-derived Monomers-based Pretreatment on Bonding to Sound and Caries-affected Dentin
Authors: Lemos MVS , Araujo-Neto VG , Lomonaco D , Mazzetto SE , Feitosa VP , Santiago SL 
Published: December 2021

SUMMARY
This study evaluated the influence of new monomers derived from cashew nut shell liquid (CNSL) applied for dentin biomodification on resin–dentin bond strength, nanoleakage, and micropermeability to sound and artificially-created caries-affected dentin. Human dentin specimens were assigned to five groups, according to the following dentin pretreatment solutions: Absolute ethanol (control), 2 wt% grape seed extract (Vitis vinifera), 2 wt% cardol [from cashew nut shell liquid (CNSL)], 2 wt% cardol-methacrylate or 2 wt% cardanol-methacrylate applied on sound and artificial caries-affected dentin. Specimens were analyzed after 24 hour or 1 year of water storage. Microtensile bond strength (μTBS) (n=6), interface micropermeability (n=3), and silver nanoleakage (n=6) were assessed using a universal testing machine, confocal laser scanning microscope, and scanning electron microscope, respectively. In sound dentin, no difference in bond strength was observed between the groups in either storage period. In artificial caries-affected dentin, pretreatment with cardol-methacrylate resulted in statistically higher bond strength than all the other treatments in both storage periods. Cardol-methacrylate treatment resulted in less nanoleakage, along with improved interfacial integrity, compared to further treatments in artificial caries-affected dentin. Regarding micropermeability analysis, all treatments depicted deficient sealing ability when applied on artificial caries-affected dentin, with the presence of gaps in the control group. In conclusion, cardol-methacrylate is a promising plant-derived monomer to reinforce the hybrid layer, since it preserved resin–dentin bond strength and improved dentin bonding, especially to caries-affected dentin, a well-known harsh substrate for adhesion longevity.
Usufructo: efectos de su transmisión entre vivos con referencia a su embargo y subasta
Authors: Masci Belen 
Published: December 2021

El usufructo es un derecho real que no ha permanecido estático con un perfil netamente asistencial, si no que por el contrario, el mismo ha adoptado un perfil también económico. Esta ampliación en su función económica social se ha traducido en la tendencia mayoritaria de las legislaciones modernas a flexibilizar la negociabilidad del usufructo y alentar su transferencia. Durante la vigencia del código velezano, la doctrina mayoritaria entendía que el usufructuario tenía la facultad de Ceder sólo el Ejercicio del Usufructo a un tercero. El Código Civil y Comercial de la Nación, en dicha marcada tendencia a promover su expansión en su utilidad y flexibilización, modificó dicha tradición jurídica permitiéndole expresamente al usufructuario, la Cesión del mismo Derecho Real de Usufructo a un tercero. Éste será precisamente el objeto de análisis del presente trabajo, su transmisibilidad por actos entre vivos y sus consecuencias, en cuanto al modo de efectivizar su embargo y ejecución forzada por parte de los acreedores del usufructuario transmitente, según se entienda que se trata de la cesión del derecho o de su ejercicio.