bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research of the Head Family Food's Conception Perceived by the Different Age Groups
Authors: Chung Hae‐Kyung , Kim Mi‐Hye 
Published: June 2021

The research aimed to provide an accurate and basic data that compares the different perceptions of head family's food and food for religious ceremonies depending on the age; the research was designed to set a right direction for the development of the traditional Korean food in order for it to be sustained and continued for future. The age diversity was: 211 participants (23.7%) in their 20 to 30s, 215 participants (33.3%) in their 40 to 50s, and 220 participants (34%) in their 60s or higher. The older generation appeared aware of the head family's definition, or 'the eldest's house of the head family', and the concrete concept of the one's family five generations ago than the head family of the younger generation. The family's role and meaning were more likely as 'succession of tradition' and as 'cultural symbols' for the younger generations; the older generations saw the family significance mainly as 'hallmark of the head family'. The experience of family ancestral rites and the head family's food apparently had a positive influence in shaping in the awareness of the head family's food. Moreover, the ones with experiences in practicing family ancestral rites approved of the necessity that the head family's food should be more popular. More positive the people were with the family's food, the more open minded they were with the possibility in which the head family's food could go mainstream. In conclusion, the positive perception of the head family's food and traditional pride resulted as a crucial environmental factor for the public support for the head family's food to become popular and more accessible to the public than in the past.This work was supported by a grant from the Korean Rural Development Administration (PJ00997602).
Probabilistic Bioactive Food Compound Intakes in the European BACCHUS Project
Authors: Pigat Sandrine , O'Connor Rosalyn 
Published: June 2021

ObjectiveThe EU funded BACCHUS project aims to develop tools and resources to study relationships between bioactive food compound intakes and cardiovascular health in humans. To handle variation and uncertainty of bioactive levels in foods a probabilistic model of bioactive intakes was used to estimate distributions of population intakes.MethodsTo assess food bioactive intake distributions in Europe, national food intake surveys were used from the UK, the Netherlands, Norway and Spain. To account for variability and uncertainty of bioactive concentrations within foods, the foods consumed were linked to discrete bioactive concentration distributions using published data on plant based foods as captured in the eBasis database. Daily bioactive population intakes were calculated using a probabilistic intake model in the Creme Nutrition® software.ResultsData shows apple (g/day) and catechin (mg/day) intakes from apples and apple products in the four countries.

Daily intakes (mg/day)
Ireland
UK
Spain
Norway

Mean (95%ile)
Mean (95%ile)
Mean (95%ile)
Mean (95%ile)

Apple + Apple Products
33 (135)
32 (129)
42 (183)
78 (300)

Catechin
1±0.1 (5.8±0.4)
1±0.1 (5.2±0.3)
1.3±0.06 (8±0.4)
2.5±0.14 (14.8±1.0)

Epicatechin
13±0.6 (56.5±3.5)
12.9±0.65 (54.5±3.6)
16.7±0.48 (73.1±2.4)
29.8±1.06 (119.6±6.0)

Epigallocatechin
22.4±0.9 (92.2±4.1)
21.7±1.0 (88.2±4.9)
28.9±0.8 (125.2±6.8)
53.1±1.8 (204.9±7.7)

Epicatechin‐gallate
0.1±0.0 (0.3±0.0)
0.1±0.0 (0.2±0.0)
0.1±0.0 (0.3±0.0)
0.1±0.0 (0.6±0.0)

ConclusionThis study enables the link between bioactive concentration levels in foods and representative population intakes, using probabilistic intake models to better estimate full intake distributions in a population.
A Review and Meta‐Analysis of Red Meat Consumption and Type 2 Diabetes
Authors: Bylsma Lauren , Miller Paula , Alexander Dominik 
Published: June 2021

This review aimed to update the state of the epidemiologic science by conducting a systematic review and meta‐analysis to estimate the potential association between red meat consumption and type 2 diabetes. A comprehensive literature search was performed to identify relevant prospective studies. Random‐effects meta‐analyses were conducted to generate summary relative risk estimates (SRREs) between intakes of total red meat and individual items and risk of type 2 diabetes. Heterogeneity was examined in sub‐group analyses. Meta regression, sensitivity, influence, and publication bias analyses were performed. A total of 20 studies were included and meta‐analyzed. Meta‐analysis of 13 independent cohorts of red meat intake and type 2 diabetes resulted in an SRRE of 1.18 (95% CI: 1.10‐1.26), but statistically significant heterogeneity was present (p‐H < 0.001, I2 = 64.08). Most meta‐analysis models produced summary associations ranging between 1.0 and 1.3, but statistically significant heterogeneity was present across the majority of the analyses. These results should be interpreted cautiously, as associations were relatively weak, confounding was significant across the individual studies, and methodological and statistical heterogeneity was considerable. It may be the interaction of dietary balance with favorable lifestyle choices that may provide the most meaningful impact on reducing the risk of type 2 diabetes.Funding provided by Meat & Livestock Australia
Functional Studies of the Putative Organic Cation Transporter SLC22A23
Authors: Chaity Nazia 
Published: June 2021

IntroductionSLC22A23 (Solute Carrier 22 family 23) is an orphan gene that encodes for a putative organic cation membrane transporter protein. The gene has been repeatedly associated to inflammatory bowel disease however; the gene structure in human and its function is still uncharacterized. Inflammatory bowel disease (IBD) is a chronic disease that is steadily increasing worldwide with the highest prevalence in Canada.ObjectivesThe proposed research aims to determine the tissue expression and characterize the subcellular location of the SLC22A23 protein, in mammalian cells.MethodsHuman SLC22A23 has been subcloned by Gateway® Technology and tagged with green fluorescent protein (GFP) and transfected in three cell lines‐ CHO, MDCK and Caco‐2. Membrane localization of SLC22A23 has been performed using confocal fluorescence microscopy. And to determine the tissue expression Real‐Time polymerase chain reaction has been used.ResultsHuman SLC22A23 protein is located in the cell membrane and is abundantly expressed in various tissues in human body with high expression in brain, kidney and intestine.ConclusionThe characterization of the SLC22A23 gene will provide the basis to determine which genetic variation is causative in the development of IBD in future studies. The correct sub‐cellular location will confirm that the human SLC22A23 is a membrane transporter protein (as known from the rat orthologous). Further functional studies of this gene will explicate the mechanism in the development of IBD and will contribute to the understanding which metabolite/nutrient imbalance plays a role in the development of IBD.
Wild Blueberry Consumption (V. angustifolium) Attenuates Local Inflammation in the Perivascular Adipose Tissue (PVAT) of the Obese Zucker Rat (OZR)
Authors: Klimis‐Zacas Dorothy , Vendrame Stefano , Kristo Aleksandra 
Published: June 2021

PVAT has emerged as critical in vasoregulation and a link between systemic and local vascular inflammation.The role of wild blueberries (WB) was examined on Phenylephrine‐induced vasoconstriction (10‐8 to 3x10‐6M) in aortic rings with or without PVAT in 12 week‐old OZRs (n=16) and their lean littermates (LZR) (n=16) on a control (C) or an 8% WB diet for eight weeks. Interleukin (IL)‐8, monocyte chemoactractant protein (MCP)‐1, tumor necrosis factor (TNF)‐a and adiponectin concentrations were measured in PVAT by ELISA.Maximal vasoconstriction force (Fmax) in aorta without PVAT was significantly lower in OZR‐C compared to LZR‐C (0.41 ± 0.05 and 0.71 ± 0.06 g, respectively). Following WB diet, a significant increase in Fmax was observed in the OZR group (0.54 ± 0.06 g). In aortic rings with PVAT, Fmax was significantly lower in all groups and all treatments (0.31 ± 0.06 OZR‐C, 0.30 ± 0.05 OZR‐WB, 0.29 ± 0.03 LZR‐C and 0.30 ± 0.04 g LZR‐WB), but no difference was observed between treatments.In PVAT, concentrations of MCP‐1, TNF‐a and adiponectin were significantly higher in OZR group compared to LZR (+102%, +108%, and +45%, respectively). Following the WB diet, concentrations of IL‐8 in PVAT were significantly lower in both OZR (–37%) and LZR (–30%), while adiponectin concentrations significantly increased in both OZR (+11%) and LZR (+16%). MCP‐1 concentrations significantly decreased (–31%) in the PVAT of OZR with WB diet.Thus wild blueberries have the potential to attenuate local inflammation in PVAT with possible implications on arterial function.Funded by the Maine Wild Blueberry Commission
Effect of Rosemary (Rosmarinus Officinalis L.) Supplementation on Cardiac Remodeling after Myocardial Infarction in Rats
Authors: Murino Rafacho Bruna , dos Santos Priscila , Gonçalves Andrea , Fernandes Ana Angelica , Gaiolla Paula , Zornoff Leonardo , Minicucci Marcos , de Paiva Sergio 
Published: June 2021

AimTo analyze the influence of rosemary (R) consumption on cardiac remodeling after myocardial infarction (MI). Material: Male Wistar rats were allocated into: 1) Sham group fed standard chow (SA0, n=6); 2) sham group fed standard chow + 0.02% of dried R (SA002, n=6); 3) sham group fed standard chow + 0.2% of dried R (SA02, n=6); 4) MI rats fed standard chow (IA0, n = 6); 5) MI rats fed standard chow + 0.02% of R (IA002, n=6); 6) MI rats fed standard chow + 0.2% of R (IA02, n=6). After 3 months, heart tissue was collected and evaluated for collagen and myocite cross seccional area (CSA), lipid hydroperoxide concentration (LH) and enzyme activity of gluthatione peroxidase (GPx), superoxide dismutase (SOD), citrato sintase (CS), lactate dehydrogenase (LDH), pyruvate dehydrogenase (PDH), β‐Acyl CoA dehydrogenase (β‐OH‐acil CoA‐DH), ATP sinthase, Complex I and II of respiratory chain. Comparisons were made by two‐way ANOVA. Values were presented as mean ± standard error. Results: MI rats showed higher CSA and collagen content, higher cardiac LH and SOD, and lower GSH‐Px and Complex I activity than Sham rats, suggesting hypertrophy, oxidative stress and mitochondrial dysfunction 3 months after MI. Rosemary supplementation with 0.02% reduced CSA. Both doses of R reduced LH, SOD and LDH, increased GSH‐Px, CS, β‐OH‐acil CoA‐DH and Complex I activity after MI. Treatment with 0.2% of R increased Complex II.ConclusionChow supplementation with rosemary attenuated cardiac remodeling after myocardial infarction in rats. Financial support: FAPESP.
Peptides from Common Bean (Phaseolus vulgaris L.) non‐Digestible Fraction Inhibit Angiotensin‐I Converting Enzyme by Interacting with Amino Acids in its Catalytic Cavity
Authors: Luna Vital Diego , Gonzalez de Mejia Elvira , Mendoza Sandra , Loarca‐Pina Guadalupe 
Published: June 2021

Five major peptides (GLTSK, LGSNK, GEGSGA, MPACGSS and MTEEY) from non‐digestible fraction (NDF) of common bean have demonstrated antiproliferative activity against HCT116 and RKO human colorectal cancer cells. The aim was to evaluate angiotensin‐I converting enzyme (ACE) inhibitory activity and antioxidant capacity of peptides in common bean. For ACE inhibition, hippuril‐histidyl‐leucine was used as substrate and the product (hippuric acid) was quantified by HPLC. Antioxidant capacity was evaluated through FRAP, ABTS and DPPH assays. The inhibition of ACE ranged from IC50=33 to 91 µg of peptide/mL. Peptides inhibited ACE activity by interacting with residues His353, Ala354, Glu384, Glu411 and Tyr523 inside the catalytic cavity of the enzyme as determined by molecular docking. The peptide MPACGSS had the highest (p<0.05) antioxidant activity: 421.6 µmol FeSO4/mg (FRAP), 561.4 μmol Trolox/mg (ABTS) and 748.4 μmol Trolox/mg (DPPH). Since ACE inhibition and antioxidant activity may be involved in colorectal chemopreventive mechanisms, the results support the notion that peptides in common bean can contribute to colon health.Supported by University of Illinois and University of Queretaro research program
β1‐Integrin, KCa3.1 and TRPC4 Channels Play Complementary Roles in Alveolar Epithelial Repair
Authors: Chebli Jasmine , Girault Alban , Prive Anik , Trinh Nguyen Thu Ngan , Maille Emilie , Grygorczyk Ryszard , Brochiero Emmanuelle 
Published: June 2021

Extensive alveolar epithelial injury and remodelling are typical features of acute respiratory distress syndrome (ARDS). It has been established that epithelial regeneration, and secondary lung oedema resorption, are crucial for the resolution of ARDS parameters. Interestingly, many evidences indicate that K+ channels are regulating epithelial repair processes; however the role of Ca2+‐activated KCa3.1 channels in alveolar repair has never been investigated before.Wound healing assays first demonstrated that the repair rates of primary rat alveolar cell monolayers were increased on a fibronectin matrix compared to non‐coated supports. KCa3.1 inhibition impaired the fibronectin‐stimulated wound‐healing rates, as well as cell migration and proliferation, but was without effect in absence of coating. We then evaluated a putative relationship between KCa3.1 and the β1‐integrin, which is activated by fibronectin. Immunofluorescence and co‐immunoprecipitation assays indicated a cellular co‐distribution and a physical link between the two proteins. In addition, KCa3.1 and β1‐integrin membrane expressions were both increased on a fibronectin matrix. We also showed increased [Ca2+]i as well as enhanced expression of TRPC4, a non‐voltage gated Ca2+ channel of the large TRP channel family, in the presence of fibronectin. Finally, wound healing assays showed that TRPC4 function is also involved in alveolar repair.Altogether, our data demonstrate for the first time a complementary role of β1‐integrin, KCa3.1 and TRPC4 channels in the regulation of alveolar repair processes.Funded by CIHR, FRQS and NSERC.
CXCR4 Gradient Sensing Generates Unique Signaling Kinetic Signatures of Akt and p53 Pathways, and Drives ERK to Invadopodia in Metastatic Breast Cancer Cells
Authors: Winant Bryce , Freed Jacqueline , Stewart Joshua , Sobel Michael , Campbell Mallory , Jancina Anastasia , Myers Kenneth , Moore Catherine 
Published: June 2021

During development, the chemokine receptor CXCR4 is essential for migration of neuronal and hematopoietic cells upon SDF gradient sensing. During disease, the SDF‐CXCR4 axis is critical for metastasis by promoting tumor cell invasion into distal SDF‐expressing organs. Despite this, the pathways downstream of motility inducing SDF gradients are unknown. Previously, we identified unique pathways associated with SDF gradient sensing, including a novel Arf6‐ERK motility pathway and a sustained MAPK pathway atypical for GPCRs but reminiscent of GFR signaling during malignancy. Here we extend these findings using microarray and IB, and identified that gradient SDF also leads to sustained Akt activation, and both the Akt and ERK pathways are inhibited by wortmannin or LY294002. Notably, both pathways regulate processes critical to malignancy including invadopodia formation. Additionally, gradient SDF promotes robust accumulation of p53, with an associated increase in Ser15 phosphorylation and implicated kinases. We predict that reduced cell cycle progression through a p53 pathway supports cell motility in response to SDF gradient sensing. We also extend our findings on the Arf6‐ERK motility pathway and determined that it mediates invasion of metastatic breast cancer cells, and that Arf6 drives ERK to pseudopodia and invadopodia upon gradient SDF treatment. These results provide insight into the biochemical pathways downstream of CXCR4 gradient sensing, with implications on the invasive phenotype of metastatic breast cancer cells.Supported by grant GM‐097718
mGluR1‐β‐arrestin 2 Signaling Mediates Induction of Excitatory Synaptic Plasticity
Authors: Eng Andrew , Hedrick Tristan , Swanson Geoffrey 
Published: June 2021

A subset of seven‐transmembrane receptors (7TMRs) couple to both G protein‐ and β‐arrestin (βarr)‐mediated signaling pathways. Our objective is to determine if group I metabotropic glutamate receptors (mGluR1 and mGluR5) engage βarr signaling during the induction of synaptic plasticity in the CNS. In these experiments we tested the role of βarr‐Src‐MAPK signaling in the induction of plasticity at the hippocampal mossy fiber‐CA3 synapse. We compared mGluR1‐dependent synaptic plasticity in patch clamp recordings from CA3 pyramidal neurons in hippocampal slices prepared from wildtype, βarr 1 and βarr 2 knockout mice. Potentiation of EPSCs induced by low frequency, paired stimulation of mossy fiber inputs to CA3 pyramidal neurons was absent in mice lacking βarr 2 (91 ± 7% n=9 for βarr 2‐/‐, 125 ± 9% n=18 for wildtype, p=0.01) but intact in mice lacking βarr 1 (128 ± 11% n=8 for βarr 1‐/‐, 122 ± 13% n=17 for wildtype, p=0.74). In contrast, high‐frequency stimulation that induced long‐term potentiation was unaffected by gene targeting of either βarr isoform (190 ± 23% n=9 for βarr 1‐/‐, 153 ± 18% n=6 for wildtype, p=0.22; 163 ± 18% n=7 for βarr 2‐/‐, 167 ± 14% n=6 for wildtype, p=0.89). Pharmacological dissection implicated ERK1/2 of the MAPK pathway (104 ± 8% n=6 for u0126‐treated slices, 139 ± 9% n=15 for vehicle slices, p=0.01), whereas the role of Src tyrosine kinases was equivocal (114 ± 8% n=9 for PP2‐treated slices, 139 ± 9% n=15 for vehicle slices, p=0.06). These data reveal a novel signaling function for βarrs when coupled to mGluR1 and therefore yield insight into new strategies for therapeutic targeting of group I mGluRs, which in recent years has been pursued for a number of neuropathologies. Research supported by R21 NS088916‐01 from NINDS to GTS.
p‐Cymene Complexed in β‐Cyclodextrin to Cancer Pain Control
Authors: Barreto Andre , Guimarães Adriana , Oliveira Marlange , Serafini Mairim , Araujo Adriano , Barreto Rosana , Quintans Jullyana , Quintans‐Junior Lucindo 
Published: June 2021

p‐Cymene (PC) is an alkylbenzene commonly found in cumin oil which has shown antinociceptive activity in animal models. Now, we evaluated the effect of encapsulation of PC in β‐cyclodextrin (PC/β‐CD) on nociception induced by tumor cells (sarcoma 180) in rodents. PC/β‐CD complex was administered (12.5 ‐ 50 mg/kg, p.o.) in mice with tumor (106 cells/25 mL, s.c.) on the hind paw, which were evaluated regarding hyperalgesia (digital von Frey apparatus), spontaneous and palpation‐induced nociception. The experimental protocols were approved by UFS Ethic Committee (CEPA: 19/11). PC/β‐CD (12.5, 25 e 50 mg/kg) was able to reduce significantly hyperalgesia (p < 0.001 or 0.05), and this effect was maintained for 24 hours after treatment. Oral administration of PC/β‐CD complex (12.5‐50 mg/kg) on alternate days also reduced spontaneous and nociception induced by palpation (p<0.001 ‐ 0.05). Free PC (50 mg/kg, p.o.) and PC no complexed with β‐CD, were unable promote significant changes in nociceptive responses. Therefore, results obtained suggest the inclusion complex containing p‐cymene and β‐cyclodextrin may improve the pharmacological characteristics of terpenoids or related compounds with potential applicability in chronic pain, such as cancer.Financial support: FAPITEC‐SE and CNPq (Brazil).
Deregulation of Bile Acid Homeostasis and Lipid Metabolism in Parenteral Nutrition Mouse Model
Authors: Zhan Le , Yang Ill , Kong Bo , Shen Jianliang , Buckley Brian , Guo Grace 
Published: June 2021

Long‐term parenteral nutrition (PN) administration can lead to PN associated liver diseases (PNALD), of which the molecular pathogenesis remains poorly understood. Animal studies in preterm piglets and several mouse models have shed light on the mechanisms underlined. To date, the roles of bile acids (BAs) and their signaling pathways involved in the development and progression of PNALD are still unclear.In the current study, we have established a mouse PN model with IV infusion of PN solution containing soybean oil based lipid emulsion. Liver mRNA profiling was determined by microarray analysis and serum BA profiling was determined by UPLC‐MS method. PN significantly decreased the mRNA levels of sterol 12‐alpha‐hydroxylase (Cyp8b1) and BA efflux genes, and increased that of cholesterol 7‐alpha‐hydroxylase (Cyp7a1). Consistently, both the levels and the percentages of primary BAs as well as total non‐12α‐OH BAs increased significantly in the serum of PN mice. In addition, PN increased the expression of several key genes involved in lipogenesis, which could be suppressed by farnesoid X receptor (FXR) and/or induced by liver X receptor alpha (LXRα).This study suggests that PN leads to deregulation of BA and lipid homeostasis, which predispose the subjects to pro‐cholestatic conditions. Determining deficiencies in BA pathway in patients may help to predict probability of PNALD development, and maintaining BA homeostasis by therapeutic agents may represent a novel treatment in the future for PNALD.
Effect of Leukotriene Modifiers on Transport Activity of Multidrug Resistance Proteins (MRPs)
Authors: Csandl Mark , Conseil Gwenaëlle , Cole Susan 
Published: June 2021

Multidrug resistance protein 1 (MRP1), MRP2, MRP3, and MRP4 are membrane transporters that mediate ATP‐dependent efflux of xenobiotics, their metabolites, and many physiological organic anions. MRP1‐4 have distinct substrate profiles; however, they can all transport the glucuronide conjugate of estradiol (E217bG). An established physiological role of MRP1 is to efflux the proinflammatory leukotriene C4 (LTC4). MK‐571 was originally designed as a leukotriene receptor 1 (CysLT1R) antagonist to treat asthma; it is also the most popular MRP1 inhibitor. However, MK‐571 is non‐specific and inhibits most MRP homologs as well as some solute carrier organic anion (SCLO) importers, limiting its usefulness as an experimental tool. Other leukotriene modifiers (LTMs) have been developed to treat inflammatory diseases but little is known of their ability to modulate MRP1 and its homologs. In this study, the effect of a series of LTMs specific for either CysLT1R or CysLT2R on E217bG uptake into MRP1, MRP2, MRP3, or MRP4‐enriched membrane vesicles was measured. The IC50 values for the 5 LTMs tested ranged from 0.91 to 25.27 uM; MRP1 and MRP4 IC50 values were the most alike. In contrast, some LTMs stimulated MRP2 and MRP3. Thus, LY171883 stimulated MRP2 and MRP3‐mediated E217bG uptake by about 2.3 and 4‐fold, respectively, while Montelukast modulated MRP2 in a biphasic manner. CysLT1R specific LTMs were generally a less potent class of MRP modulators. These data suggest that like MK‐571, most LTMs are non‐selective modulators of MRP1‐4 transport, and should therefore be used with caution because of their potential to confound data interpretation. Supported by Canadian Institutes of Health Research MOP‐133584