bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Transplant Recipient and Donor Organ Type 2 Diabetes Polygenic Risk Scores on the Development of Early Post-Transplant Diabetes
Authors: Shaked Abraham , Loza Bao-Li , Loon Elisabet , Olthoff Kim , Guan Weihua , Jacobson Pamala , Oetting William , Israni Ajay , Fishman Claire , Testa Giuliano , Trotter James , Klintmalm Goran , Naesens Maarten , Asrani Sumeet , Keating Brendan 
Published: August 2021

Abstract
Post-transplant diabetes mellitus (PTDM) is a complication which reduces allograft and recipient life-span. Polygenic risk scores (PRS) robustly show association with greater type 2 diabetes (T2D) development risk. We examined T2D-PRS in transplant recipients and donors using genome-wide genotyping in 1581 liver recipients, and 1555 donors and 2062 kidney recipients and 533 donors from four centers. Liver and kidney recipient T2D-PRS was associated with pre-transplant T2D and PTDM development. Liver donor, but not kidney donor, T2D-PRS was an independent risk factor for PTDM development. Inclusion of a combined liver recipient and donor T2D-PRS significantly improved PTDM prediction vs clinical characteristics-only models: AUC (95%CI): 67.6% (64.1% − 71.1%) vs. 62.3% (58.8% − 65.8%), p = 0.0001. Liver recipients in the highest quintile of recipient-donor combined T2D-PRS had the greatest PTDM risk: OR (95%CI) = 3.22 (2.07–5.00), p = 1.92E-07, compared to the lowest quintile. T2D-PRS allows identification of transplant candidates with high PTDM risk, for whom early preemptive diabetes management is warranted. Pre-transplant knowledge of donor T2D-PRS in the setting of living liver donation should optimized selection of donors to reduce PTDM.
MiR-664b-3p Inhibited Triple-Negative Breast Cancer Cell Growth Via Targeting BRIP1
Authors: Jin Qiu , Lin Bo , Zhao Wenhui , Ji Runyuan 
Published: August 2021

Abstract
BackgroundMany studies indicate that microRNAs (miRNAs) play a crucial role in modulating the development and progression of triple-negative breast cancer (TNBC). However, miR-664b-3p affections on the TNBC functions and mechanisms are still unknown. The purpose of our study was to clarify the effects of miR-664b-3p in cellular TNBC development and progression.MethodsIn our study, the expressions of miR-664b-3p in cell lines and tissueswere tested by real-time PCR (RT-PCR), immunofluorescence, H&E and immunohistochemistry staining. CCK-8 assay, colony formation, EdU, flow cytometry apoptosis, wound scratch, Transwell assays were applied to explore the cell functions. The targeted relationship between miR-664b-3p and its target BRIP1 was determined by dual-luciferase reporter assay and rescue experiments. ResultsWe observed that miR-664b-3p was significantly decreased in TNBC cell lines. Overexpression of miR-664b-3p could observably inhibit cell proliferation, migration, invasion and induced apoptosis in vitro. Meanwhile, miR-664-3p suppressed TNBC tumor growth in vivo. Furthermore, luciferase reporter assays identified the interaction between 3’UTR of BRIP1 and miR-664b-3p. Moreover, we investigated the mechanisms underlying the effect of miR-664b-3p on cell functions, and the result showed that miR-664b-3p inhibited cell proliferation, invasion and accelerated apoptosis by targeting BRIP1.ConclusionFrom the above, our findings indicated that miR-664b-3p played a significant role in TNBC progression by targeting BRIP1, providing new therapeutic targets for diagnostic in TNBC.
Streamflow forecasting based on the hybrid decomposition-ensemble model
Authors: Sun Xiaomei , Zhang Haiou , Wang Jian , Shi Chendi , Hua Dongwen , Li Juan 
Published: August 2021

Abstract
Reliable and accurate streamflow forecasting plays a vital role in the optimal management of water resources. To improve the stability and accuracy of streamflow forecasting, a hybrid decomposition-ensemble model named VMD-LSTM-GBRT, which is sensitive to sampling, noise and long historical changes of streamflow, was established. The variational mode decomposition (VMD) algorithm was first applied to extract features, which were then learned by several long short-term memory (LSTM) networks. Simultaneously, an ensemble tree, a gradient boosting tree for regression (GBRT), was trained to model the relationships between the extracted features and the original streamflow. The outputs of these LSTMs were finally reconstructed by the GBRT model to obtain the forecasting streamflow results. A historical daily streamflow series (from 1/1/1997 to 31/12/2014) for Yangxian station, Han River, China, was investigated by the proposed model. VMD-LSTM-GBRT was compared with respect to three aspects: (1) Feature extraction algorithm; ensemble empirical mode decomposition (EEMD) was used. (2) Feature learning techniques; deep neural networks (DNNs) and support vector machines for regression (SVRs) were exploited. (3) Ensemble strategy; the summation strategy was used. The results indicate that the VMD-LSTM-GBRT model overwhelms all other peer models in terms of the root mean square error (RMSE=36.3692), determination coefficient (R 2 =0.9890), mean absolute error (MAE=9.5246) and peak percentage threshold statistics (PPTS(5)=0.0391%). The addressed approach based on the memory of long historical changes with deep feature representations had good stability and high prediction precision.
Price, Quality, and Market Dynamics of Malaria Rapid Diagnostic Tests: Analysis of Global Fund 2009–2018 Data
Authors: Wittenauer Rachel , Nowak Spike , Luter Nick 
Published: August 2021

Abstract
Background: Rapid diagnostic tests (RDTs) for malaria are a vital part of global malaria control. Over the past decade, RDT prices have declined, and quality has increased. However, the relationship between price and product quality and their larger implications on the market have yet to be characterized. We sought to use purchase data from the Global Fund together with product quality data from the World Health Organization and Foundation for Innovative New Diagnostics (WHO-FIND) Malaria RDT Product Testing Programme to understand three unanswered questions: 1) Has the market share by quality of RDTs in the Global Fund’s procurement orders changed over time? 2) What is the relationship between unit price and RDT quality? 3) Has the Global Fund procurement market become more concentrated over time?Methods: We merged data from 10,075 procurement transactions in the Global Fund’s database, which includes year, product, volume, and price, with product quality data from all eight rounds of the WHO-FIND program, which evaluated 227 unique RDT products. To describe trends in market share by quality level of RDT, we used descriptive statistics to analyze trends in market share from 2009–2018. We then applied a generalized linear regression model to characterize the relationship between price and panel detection score (PDS), adjusting for order volume, year purchased, product type, and manufacturer. Third, we calculated a Herfindahl-Hirschman Index (HHI) score to characterize the degree of market concentration.Results: Lower-quality RDTs have lost market share between 2009-2018, as have higher-quality RDTs. We find no statistically significant relationship between price per test and PDS when adjusting for order volume, product type, and year of purchase. The HHI was 3,570, indicating a highly concentrated market.Conclusions: Advancements in RDT affordability, quality, and access over the past decade risk stagnation if health of the RDT market as a whole is neglected. Our results suggest that this market is highly concentrated and that quality is not a distinguishing feature between RDTs. This information adds to previous reports noting concerns about the long-term sustainability of this market. Further research is needed to understand the causes and implications of these trends.
Improvement to Gene Editing in Neospora Caninum: Knockout ku80 to Increase the Efficiency of Homologous Recombination
Authors: Wu Kaijian , Song Xingju , Wu Yayun , Yang Xu , Liu Jing , Liu Qun 
Published: August 2021

Abstract
Background: The CRISPR/Cas9 technology based on homologous recombination has been widely used for gene editing in organism. Gene deletion of ku80 complex can increase the efficiency of homologous recombination(HR), or gene editing by CRISPR/Cas9 in Arabidopsis thaliana and Toxoplasma gondii, which has remained elusive in Neospora caninum. Methods: Here, we knocked out the ku80 in Nc1 strain, named Δku80, using CRISPR/Cas9. In vitro, phenotype assays, including plaque formation, invasion, replication and egress were carried out to determination of the growth of Δku80. In vivo, we respectively injected BALB/c mice with different doses of NcKU80 and Nc1, and measured the survival curves and parasites burdens of mice infected. To test the efficiency of HR, Δku80 and Nc1 strains were transfected with the same HA-tagged plasmids, and the percentage of parasites with HA-tagged was investigated.Results: It is showed that the growth and virulence of Δku80 kept unaffected, while the efficiency of gene targeting via double-crossover at several genetic loci was increased 2 to 3 fold in Δku80. Conclusions: It is concluded that Δku80 can be used as an effective strain for rapid gene editing in N. caninum.
LDL-C/HDL-C and carotid plaques in patients with coronary heart disease: A Chinese cohort study
Authors: Li Zhu , Cheng Qi , Liu Yijia , Cheng Xufeng , Wang Shuo , He Yuanyuan , Wang Xu , Huang Mengnan , Li Yue , Xue Xiaoxue , Xu Yilan , Li Lin , Zheng Yanchao , Yang Rongrong , Gao Shan , Yu Chunquan 
Published: August 2021

Abstract
Background: Evidence of the association the low-/high-density lipoprotein cholesterol ratio (LDL-C/HDL-C) with the carotid plaques remains limited. The purpose of this study is to examine the association between LDL-C/HDL-C and carotid plaques of coronary heart disease (CHD), and to study what extent a healthy lifestyle reduces the risk of carotid plaques.Methods: In this large-scale and multi-center retrospective study, a total of 9,426 participants to explore the association between LDL-C/HDL-C and carotid plaques. No smoking and no drinking were considered healthy lifestyle. Generalised estimating equation models and conditional logistic regressions were used in statistical analyses.Results: In all the 9,426 participants, there were 6,989 (74.15%) patients having identified carotid plaques. High levels of LDL-C/HDL-C had a higher risk of carotid plaques than other lipid variables (OR:1.63; 95%CI:1.43-1.86). In stratified analyses by LDL-C/HDL-C triplet, participants in the LDL-C/HDL-C (＞3mmol/L) group had a higher risk of carotid plaques compared to other two groups. Compared with the unfavourable lifestyle, intermediate lifestyle or favourable lifestyle was associated with a significant 30% or 67% decrease in carotid plaques risk among patients with the LDL-C/HDL-C(＞3mmol/L) respectively. There were significantly additive and multiplicative interactions between lifestyle and LDL-C/HDL-C on carotid plaques.Conclusion: Our findings provide evidence that a high level of LDL-C/HDL-C can increase the risk of carotid plaques in patients with CHD. And adhering to a healthy lifestyle has additive beneficial effects on reducing the risk of carotid plaques.
Research on Micro-EDM Discharge State Detection Technology Based on Inter Electrode Impedance Variation Characteristics
Authors: Chen Rui , Hu Bo , Zhang Yongbin , Liu Guangmin , Dai Yue , Shen Jie 
Published: August 2021

Abstract
Aiming at the problem that it is difficult to detect the discharge state of narrow pulse width and small duty cycle pulse, this paper studies the variation characteristics of inter electrode impedance in micro-EDM process, puts forward a detection method to distinguish different discharge states in micro-EDM based on the change of electrical signal caused by the change characteristics. The influence of pulse width and duty cycle on the quality of micro-EDM is analyzed. The surface machining quality of workpiece can be improved obviously by compressing the pulse width of pulse power supply. And designs a discharge state detection system based on inter electrode impedance variation characteristics. Experiments verify the detection method, and test the machining effect of the control strategy through the trial machining experiment, which ensures that the micro-EDM process is efficient and stable.
Effects of Processing Parameters on the Surface Quality of Wrought Ni-based Superalloy by Ultrasonic Assisted Electrochemical Grinding
Authors: Ma Yucai , Yin Yingyue , Zhang J H , Huo Jinxing 
Published: August 2021

Abstract
Ni-based GH625 superalloy has been widely employed in the aerospace industry due to its high strength, outstanding corrosion resistance and high temperature resistance. A novel hybrid processing method of ultrasonic-assisted electrochemical grinding (UAECG) can achieve the high-effective removal rate for difficult-to-process materials and thereby avoids the stray corrosion during the process. This study systematically investigated the electrochemical dissolution behaviors of GH625 alloy at low current density and effects of processing parameters on its surface roughness. A qualitative model was proposed to further reveal its removal mechanism for GH625 alloy during UAECG process. Polarization curves depicted that an efficient and stable electrochemical dissolution was achieved at an appropriate temperature (20°C) and concentration (10 wt.%) of NaNO 3 electrolyte. The findings also revealed that selective corrosion preferentially occurred on the grain boundary or near the NbC carbides under different current density corrosion circumstance. Compared with ECG process, the excellent surface quality ( R a = 0.37 μm) and taper of the small holes (taper = 0.04 ±0.005°) are obtained at the optimized condition of pulse voltage of 5.8V, feed rate of 0.6mm /min, cathode speed of 12kr /min and ultrasonic drive amplitude of 60 % by UAECG technology.
Sustainable Policies for Supply Chain During the Digital Economic Crisis Due to Epidemics
Authors: Su Yong , Fu Qinghua , Sharma Ashutosh , Jumani Awais Khan 
Published: August 2021

Abstract
One of the most exciting issues in the digital economy has been sustainability supply chain management. There are many articles on this area and several different viewpoints. This work focuses on assessing the sustainability of supply chain management based on two main measurements. Firstly, the value of ecological, environmental, and social measurement indicators and the extent of the challenge in collecting information. The objective of this article is to improve the precision of the assessment. A Sustainable Policies for Supply Chain Management (SP-SCM)is proposed in this article. The suggested framework is a synthesis of quality function deployment (QFD) with plithogenic set grouping processes.A summation function is applied: the perception of the decision-maker of criteria required to assess the viability of the supply chain, the measurement criteria dependent on the specifications, and the difficulties in collecting details. This research submitted a real-world test case on the sugar industry to test the proposed framework. In evaluating the sustainability of the supply chain policy, the findings revealed the performance efficiency of the proposed system.
Association of Anti-oxidative Capacity of HDL With Subclinical Atherosclerosis in Non-alcoholic Fatty Liver Disease
Authors: Karami Sara , Poustchi Hossein , Sarmadi Negar , Radmard Amir Reza , Yari Fatemeh Ali , Pakdel Abbas , Shabani Parisa 
Published: August 2021

Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) patients are at the substantial risk for the for developing cardiovascular disease (CVD). High-density lipoprotein (HDL) is well known to have protective effects against the development of atherosclerotic CVD. One of the major antiatherogenic effect of HDL is its anti-oxidative function. Objectives: This study investigated the association of anti-oxidative function of HDL with NAFLD and CVD risk.Methods: A total of 143 subjects including 51 NAFLD and 92 control subjects were included in this cross-sectional study. HDL oxidative index (HOI) was determined spectrophotometrically using a cell-free method in the presence of a fluorescent substrate dichlorofluorescein diacetate (DCFDA). Paraoxonase 1 (PON1) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) plasma levels were assessed in both groups.Results: The subjects with impaired HDL anti-oxidative function (HOI≥1) had lower PON1 paraoxonase activity, lower SOD activity, higher MDA levels and higher carotid intima-media thickness (cIMT) values compared to the controls. HDL oxidative index (HOI) was positively correlated with MDA levels and cIMT and negatively correlated with SOD activity. NAFLD patients with HOI≥1 had elevated serum levels of liver enzymes and higher LS values.Conclusions: Alteration in circulating oxidative stress markers may diminish the anti-oxidative function of HDL. Impaired anti-oxidative capacity of HDL might be related to NAFLD severity and subclinical atherosclerosis in NAFLD patients.
Simulation Study on the Effect of Coal Seam Hydraulic Fracturing to Increase Permeability
Authors: Wang Kai , Zhang Guodong , Wang Yanhai , Zhang Xiang , Li Kangnan , Guo Wei , Du Feng 
Published: August 2021

Abstract
Hydraulic fracturing is mainly used to improve coal seam permeability, extract coal seam gas and prevent coal-gas outburst. However, in the aspect of anti-reflection effect, it is judged by the method of field inspection, and there is no good prediction method before the engineering application. In this work, PFC2D software is used to simulate the process of coal seam hydraulic fracturing, and the results are introduced into the mathematical coupling model of the interaction between coal seam deformation and gas flow for analysis. The results show that the particles are moving towards the direction of the minimum principal stress, the moving speed of the particles at the crack tip increases, and the crack will finally expand towards the direction of the maximum principal stress. The gas pressure decline rate and permeability increase rate of the fractured model are both higher than that of the unfractured model. With the increase of extraction time, the decrease rate of gas pressure and the increase rate of permeability both decrease rapidly and gradually approach 0. The longer the hydraulic fracturing time is, the more complex the fracture network is, and the faster the gas pressure drops. However, the difference between the horizontal and vertical gas pressure drops will become larger and larger, and the impact of fracturing on gas drainage effect will gradually become smaller. With the increase of fracturing time, the difference of permeability increase at different monitoring points becomes larger and larger, but with the increase of gas drainage time, the difference will gradually decrease. The higher the initial void pressure is, the faster the gas pressure drops and the greater the permeability increase. However, the influence of the initial void pressure on the permeability increase will gradually become smaller. The research results could provide certain guidance for the prediction of anti-reflection effect of hydraulic fracturing in underground coal mines.
Simulation analysis of the significance and interaction of influencing factors on mixing uniformity of double drum recycling mixing plant
Authors: Ma Dengcheng , Chen Chao , Liu Chengqi 
Published: August 2021

Abstract
The mixing uniformity of the double drum recycling mixing plant has an important effect on the quality of recycled asphalt mixture. The mixing uniformity of the double drum recycling mixing plant not only depends on the axial installation angle of the blade, the radial installation angle of the blade, the phase angle of the stirring arm, the inclination angle of the drum, and the rotation speed of the drum, but also on the interaction between these factors. In order to further clarify the relationship between the mixing uniformity and the above factors, the significance and interaction of influencing factors of mixing uniformity were studied based on EDEM and response surface methodology (RSM). The result shows that the factors affecting the mixing uniformity of the aggregates are significantly different when the aggregate size is different. Therefore, when studying the mixing uniformity of a mixing plant, it is not comprehensive to conduct research on a certain factor in a single-sided manner. Instead, the main factors and the interaction between the factors should be considered comprehensively.
Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activity
Authors: Wang Yuya , Xue Xiaoling , Dan Kena , Yang Xiongbo , Yang Qingqing , Feng Xujiao , Yang Jing , Chen Bangtao 
Published: August 2021

Abstract
BackgroundThe increase of inflammation-inducing enterobacteria was recently observed in severe hand, foot, and mouth disease (HFMD) caused by Enterovirus A71 (EV-A71). This study aimed to verify the occurrence of bacterial translocation (BT) and further explore the contributory role of BT to severity of EV-A71-mediated HFMD cases. MethodsSerum specimens from 65 mild and 65 severe EV-A71-associated HFMD cases and 65 healthy children were collected. EV-A71 VP1 in serum, inﬂammatory mediators including C-reactive protein, IL-1β, IL-6, interferon-γ and tumor necrosis factor-α, BT related biomarkers including Claudin-3, intestinal fatty acid binding protein, lipopolysaccharide (LPS), soluble CD14(sCD14) and endotoxin core antibody were measured by ELISA. Bacterial DNA (BactDNA) fragments were quantified by quantified PCR (qPCR). Rhabdomyosarcoma (RD) cells, infected with LPS-pre-incubated EV-A71 or transfected with plasmid containing viral 2A pro or mRNA containing viral internal ribosomal entry site (IRES), were post-treated with or without LPS in vitro . EV-A71 RNA and viral or cellular proteins were determined by qPCR and western blot, respectively. ResultsCompared to mild HFMD patients, remarkably higher inﬂammatory mediators as well as BT-related biomarkers except BactDNA were observed in severe HFMD cases (all P <0.05). In severe HFMD group, circulating concentrations of LPS and sCD14 showed statistical correlations with inflammation indices (all P <0.05), serum levels of EV-A71 VP1 were found to be positively correlated with serum LPS ( r =0.341, P =0.005) and serum sCD14( r =0.458, P <0.001). In vitro , EV-A71 attachment and internalization were only slightly promoted by LPS pre-incubation; however, EV-A71 proliferation and viral 2A pro -mediated IRES activity were significantly accelerated by LPS post-treatment. ConclusionsOur results collectively indicate that gut-derived translocating LPS contributes to the severity of EV-A71-induced HFMD by driving inflammatory response and viral proliferation via viral 2A pro -mediated IRES.