bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of GPR55 receptor in bovine sperm capacitation
Authors: Lottero‐Leconte Raquel , Lara Angela , Plaza Jessica , Arroyo‐Salvo Camila , Bogetti María Eugenia , Rivolta Amada Eugenia Ynsaurralde , Dellavalle Franco , Sengiali Fiamma , Cetica Pablo , Rio Sofía , Zalazar Lucia , Cesari Andreína , Miragaya Marcelo , Morado Sergio , Perez‐Martinez Silvina 
Published: January 2025

AbstractBackgroundEndocannabinoids like anandamide (AEA), among other lipids, are recognized signaling molecules that participate in reproductive events.ObjectivesOur aims were to characterize orphan G protein‐coupled receptor (GPR55) presence; investigate GPR55 activation by AEA and determine GPR55 role in the bovine sperm function.Materials and methodsGPR55 presence was assessed by immunocytochemistry. Protein kinase A (pPKA) and PKC (pPKC) substrates, pERK1/2, G/F‐actin were determined by Western blotting, activation of RAC‐1 by pull‐down assay, F‐actin and acrosomal exocytosis by fluorescence microscopy, sperm motility by optic microscopy and computer‐aided sperm analysis and fertilizing ability by in vitro fertilization (IVF).ResultsWe detected GPR55 in spermatozoa at T0, after incubation in non‐capacitating and capacitating (presence of AEA) conditions and upon release from oviductal epithelia. AEA induced an increase in pPKA and pPKC, while CID16020046 (CID), selective GPR55 antagonist, prevented this effect. Incubation with H89, PKA inhibitor, significantly decreased pPKC, while Gö6983, a PKC inhibitor, partially reduced pPKA. pPKA remained elevated at 15‐ and 45‐min incubation, while pPKC decreased at 15 and increased at 45 min. CID prevented pPKC increase at 5 and 45 min and decreased pPKA at 45 min. RAC‐1 and F‐actin increase induced by AEA was prevented by CID. Variations in two progressive motility kinematic parameters were observed with AEA and/or CID. Sperm pretreatment with AEA increased the rate of cleaved embryos and CID prevented this effect.DiscussionWe demonstrated that GPR55 activation by AEA induces time‐dependent signaling pathways involving pPKA and pPKC during bovine sperm capacitation. AEA regulates actin polymerization through GPR55 activation, suggesting the receptor participates in cytoskeleton remodeling, and yielded higher IVF rates. Also, sperm pre‐incubation with molecules like AEA involved in capacitation could improve the embryo development.ConclusionWe have demonstrated GPR55 presence in bovine spermatozoa. The regulation of PKA and PKC and of molecules associated with cytoskeletal dynamics, such as RAC‐1 and actin, by GPR55 is closely related to sperm motility and acrosomal exocytosis.
Infants admitted to Danish neonatal units demonstrate satisfactory growth independent of feeding type at discharge
Authors: Honoré Karina Dyrvig , Jespersen Jonas Sveen , Zachariassen Gitte 
Published: January 2025

AbstractAimThe aim was to investigate feeding type at discharge; exclusively breastfeeding (EBF), mixed breastfeeding (MBF), and formula milk feeding (FMF), factors associated with feeding type, and changes in weight‐for‐age z‐score (ΔWAZ) in infants admitted to Danish neonatal units.MethodsUsing data from the Danish National Quality Database for Births and the Danish Newborn Quality Database, we included 8639 mother‐infant dyads admitted ≥5 days between February 2019 and December 2021. We used logistic regression to investigate associations between maternal and infant factors and feeding type, and descriptive statistics to describe ΔWAZ and feeding type at discharge.ResultsOf all infants 59.1% were EBF, 16.9% MBF and 24.0% FMF at discharge. Gestational age <37 weeks, caesarean section, multiple births, small for gestational age, weeks at hospital, ≥6 h before skin‐to‐skin contact, and few weeks at hospital were associated with failure to EBF at discharge. Median (min–max) ΔWAZ in EBF, MBF and FMF infants was −0.44 (−4.78 to 4.88), −0.43 (−3.47 to 4.42) and −0.39 (−3.54 to 4.03), respectively. ΔWAZ was higher in EBF compared to FMF infants, p‐value 0.01, but no significant difference in ΔWAZ between MBF and FMF infants, p‐value 0.06.ConclusionDanish newborn infants demonstrated satisfactory growth during admission to the neonatal unit, independent of feeding type at discharge. Rates of exclusively breastfeeding need improvement.
Families’ experiences of housing and housing supports within the context of parental incarceration
Authors: Fanning Kerrie , Kerr Margaret , Charles Pajarita , Poehlmann Julie 
Published: January 2025

AbstractRates of parental incarceration and family homelessness continue to rise in the United States, and many families experience both of these risks concurrently. Both parental incarceration and homelessness independently relate to negative outcomes for children and families, with numerous studies documenting families’ experiences of each risk independently. Despite families’ increased risk for experiencing co‐occurring parental incarceration and housing instability, little is known about families’ experiences within this complex context of risk. This qualitative study explores currently incarcerated parents’ and their children's at‐home caregivers’ perceptions of their experiences of homelessness and housing instability during the year prior to the parent's current incarceration. Through semi‐structured interviews with 16 jailed parents and caregivers, families describe their perception of their experience for themselves and for their children and identify challenges and support they encountered. Using multiperspectival interpretative phenomenological analysis, emerging group experiential themes clustered within four overarching constructs: jailed parent/caregiver‐focused, child‐focused, family‐level, and exosystem‐level experiences. Overall, families’ descriptions capture the complexity of their cascading risk experiences, with impacts permeating through the family system. Implications for programming and policies addressing parental incarceration and homelessness are addressed.
Articulating populism in the Philippines: The rhetorical strategies of Joseph Estrada and Rodrigo Duterte
Authors: Talamayan Fernan 
Published: January 2025

AbstractThe article compares the rhetorical strategies of two of the most popular post‐martial law presidents in the Philippines, Joseph Estrada (1998–2001) and Rodrigo Duterte (2016–2022). Adopting Laclau's definition of populism and localizing De Cleen and Stavrakakis's analysis of socio‐spatial dichotomies, I examine the political logic that guided the consolidation of the people and the formation of antagonistic frontiers during the Estrada and Duterte presidencies. I identify the nodal points in their articulation of sympathy, compassion, elite opposition, political will, and belongingness, and plot them on a down/up and in/out axes. In uncovering the operation of populist logic in their political speeches, the article finds that leveraging people's frustrations and unsatisfied demands reinforced Estrada's and Duterte's ability to mobilize support and project themselves as the people's protector and champion. Through an in‐depth interrogation of their populist articulation, the article contributes to a more nuanced understanding of populism's durability and enduring appeal.
Same story, different endings: clinical course and outcomes of two dogs treated differently for delayed fulminant pulmonary haemorrhage 20 h after eastern brown snake (Pseudonaja textilis) envenomation
Authors: Mak HY , Hardjo S 
Published: January 2025

This report presents the clinical course and outcomes of two dogs that were treated differently upon recognition of respiratory distress due to delayed fulminant pulmonary haemorrhage (DFPH) 20 h after eastern brown snake envenomation. Two dogs from the same household were likely envenomated at the same time. Pulmonary haemorrhage was diagnosed based on pleural and lung ultrasound, decreasing packed cell volume and haemoptysis. Case 1 received a total of 7000 units of brown snake antivenom within the first 10 h after envenomation. The dog developed severe respiratory distress and anaemia approximately 20 h after envenomation. A decision to administer fresh whole blood was made, however, a marked deterioration in clinical signs occurred during the acquisition of blood. The dog was euthanased due to hypoxemic and hypercapnic respiratory failure. Case 2 received similar treatment to case 1 before DFPH. Case 2 developed moderate pleural effusion along with pulmonary haemorrhage after hospital admission. The dog was noticed to have respiratory distress approximately 1 h after case 1. Fresh frozen plasma was administered within 30 min, followed by packed red blood cells and autotransfusion, and the dog survived. These two cases are used as a foundation to discuss the pathophysiology of DFPH and its relation to the clinical signs. Furthermore, retrospective analysis of diagnostics and alternative approaches proposed, may assist clinicians in early recognition and optimal treatment of DFPH.
Evaluation of Heat Shock Effects on Seedling Emergence and Seed Bank Viability in 18 Colombian Dry Forest Species
Authors: Barras Rodriguez José David , Zuñiga Cespedes Bladimir , Castellanos Barliza Jeiner de Jesus 
Published: January 2025

ABSTRACTAimHigh temperatures during forest fires can cause significant damage to tropical dry forest areas and alter their ecological stability, particularly by affecting seed viability and seedling emergence. This study evaluates the seedling emergence response of 18 dry forest species to fire‐simulated temperatures, aiming to assess their potential for restoration in fire‐prone Colombian ecosystems.LocationThe seeds used in this study were obtained from three tropical dry forests in Colombia.MethodsA total of 9832 seeds from 18 dry forest species were collected directly from the soil seed bank in three tropical dry forests in Colombia. These seeds were then exposed to simulated forest fire temperatures (100°C, 150°C, and 200°C) for 10 min. Seed viability was analyzed using the 2,3,5‐triphenyl tetrazolium chloride reagent (tetrazolium test) and assessed using a generalized linear model. Seedling emergence and mean emergence time were evaluated using one‐way analysis of variance (ANOVA) with temperature treatments as factors.ResultsThe study revealed that seedling emergence significantly decreased with higher heat shock temperatures. Notably, Hura crepitans and Parkinsonia aculeata tolerated temperatures up to 100°C, while Caesalpinia pulcherrima and Enterolobium cyclocarpum showed increased emergence at that temperature. Based on their emergence responses, species were classified as stimulated, tolerant, sensitive, or vulnerable. Seed viability declined with rising temperatures, and the mean emergence time increased in species like Cordia alba, Crescentia cujete, and Lonchocarpus violaceus.ConclusionsThis study shows that heat shocks at 150°C and 200°C significantly reduced seed bank viability for most Colombian dry forest species. However, Caesalpinia pulcherrima and Enterolobium cyclocarpum were stimulated by 100°C heat shocks, while Hura crepitans and Parkinsonia aculeata showed no adverse effects. Vulnerable species like Coccoloba acuminata and Pithecellobium dulce exhibited no viable seeds at higher temperatures, suggesting potential local extinctions. These results emphasize the need to focus on heat‐tolerant species for restoration efforts in fire‐prone ecosystems.
Intra‐articular corticosteroids: Systematic review of effects on osteoarthritis and joint health
Authors: Tokawa P. K. A. , Baccarin R. Y. A. , Zanotto G. M. 
Published: January 2025

SummaryBackgroundThe importance of intra‐articular corticosteroid treatment for osteoarthritis, with its possible adverse effects, requires a comprehensive review.ObjectivesThis review answers the following questions: (1) What evidence is available regarding the symptom‐modifying and disease‐modifying changes related to a single intra‐articular corticosteroid injection for treating osteoarthritis in horses and humans? (2) What evidence suggests corticosteroids are detrimental to equine joint health?Study designSystematic review.MethodsA systematic search was conducted in June 2022 in PubMed, CAB and the Web of Science. Inclusion criteria were applied to titles and abstracts. For each question, further criteria were applied. The risk of bias was assessed according to the study design.ResultsSearches generated 6417 titles. Twenty‐three articles fit all inclusion criteria for single‐injection corticosteroid treatments and 21 were included for their relevance to corticosteroid effects on joint health. Single‐injection protocols lead to short‐term symptom‐modifying osteoarthritic changes with conflicting results regarding disease‐modifying osteoarthritis effects. Healthy joints demonstrated disturbances in metabolism and tissue changes both in vivo and in vitro after corticosteroid. A dose‐dependent effect was found in vitro.Main limitationsThere is a lack of studies regarding the questions, primarily for equine species. Furthermore, the majority of studies were rated as ‘unclear risk of bias’.ConclusionsSymptom‐modifying osteoarthritic changes after a single injection are short‐term, and a consistent disease‐modifying effect on osteoarthritis has yet to be established. Joint health is disturbed by corticosteroids as their effects on normal joints show adverse changes in tissues and metabolism.
Quality of caring interactions between nurses and patients during the COVID‐19 pandemic: A cross‐sectional survey with nurses and patients in acute and community care
Authors: Ernst Jutta , Petry Heidi , Naef Rahel 
Published: January 2025

AbstractAimsTo describe the characteristics and quality of caring interactions between nurses and patients during the earlier phases of the COVID‐19 pandemic in acute and home care settings.BackgroundNurse–patient interaction (NPI) plays an important role in effective, person‐centered care delivery and has been impacted by the COVID‐19 pandemic.MethodsThe survey was part of a multimethod study and used a cross‐sectional design. It included both nurses and patients receiving care during the first wave of the COVID‐19 pandemic (March–June 2020) at a university‐affiliated tertiary care hospital and two large public home care agencies. Data were collected from July to October 2020, using the Individualized Care Scale (ICS), patient and nurse versions. The ICS is a 5‐point Likert‐type scale divided into two dimensions: ICS‐A and ICS‐B with 17 items each. The ICS‐A subscale assesses patients’ or nurses’ perceptions of patient individuality as supported by specific nursing activities. The aim of the ICS‐B subscale, conversely, is to assess perceptions of how individuality is maintained in the care either provided or received. Inferential statistics were used for comparisons between settings and populations. The study followed the STROBE checklist for cross‐sectional studies.ResultsThe survey was completed by 443 nurses and 295 patients. Patients’ perception of “nurses support their individuality” was moderate (ICS‐A 3.42, range 1–5), as was their perception of “nurses provide individual care to patients” (ICS‐B 3.89, range 1–5). Nurses’ own perception of supporting individuality was found to be high (ICS‐A 4.30, range 1–5), as was the mean score of their actual provision of individual care (ICS‐B 4.21, range 1–5), which was statistically significantly higher than the ratings of patients. Nurses working in home care rated individuality and individual care statistically significantly higher than those working in acute care.ConclusionThe findings suggest that during the earlier phases of the pandemic, Swiss nurses perceived their support of patient individuality and individual care to be higher than their European peers had prior to the pandemic and higher than patients did. However, those engaged in the provision of care within a hospital setting perceived the level of individuality and individual care to be lower than those engaged in the provision of care within a home care setting.Implications for nursing and health policyIt is evident that nurses’ efforts and contributions to the quality of care require organizational recognition and support. Their commitment should be recognized and supported by leadership and policymakers.
Quantifying nano‐domain size and orientation in relaxor ferroelectrics using transmission electron microscopy
Authors: Brichta Nathan M. , Tegg Levi , Giles Luke W. , Daniels John E. , Cairney Julie M. 
Published: January 2025

AbstractFerroelectric materials contain regions of uniform electrical polarization, known as domains. In Pb‐based relaxor‐ferroelectric perovskite oxides, these domains can be as small as a few nanometers, making standardized analysis of domain sizes challenging. Here, we present two methods for the quantitative analysis of domain sizes from transmission electron micrographs. One method uses statistical analysis of multiple line profiles, and the other analyses the intensity of streaks in the Fourier transform of the image. The techniques provide equivalent results for bright‐field images free of other microstructure or aberrations, while the Fourier method is much simpler to apply.
A Cross Section Study on the Relationship Between Nurse‐Reported Missed Care, Emotional Labour, and Compassion Fatigue in Intensive Care Units
Authors: Jia Wanshun , Zhang Zeyi , Zhang Qi , Chen Xue , Fang Jinxia , Cao Heng 
Published: January 2025

ABSTRACTAim(s)To investigate the correlation between compassion Fatigue, emotional labour and missed nursing care among intensive care unit nurses.DesignA cross‐sectional design.MethodsData were collected from two hospitals in Shandong Province, China, from July to August 2024. A total of 226 intensive care unit nurses completed the questionnaires. The completion rate for the surveys was 98%. The Demographic Characteristic Questionnaire, Chinese version of Compassion Fatigue‐Short Scale, Chinese version of Emotional Labor Scale and Chinese version of Missed Nursing Care Questionnaire were used for investigation. Descriptive statistics, Multiple‐linear regression and Spearman correlation analyses were used for data analysis.ResultsThe mean (SD) scores of compassion fatigue, emotional labour and missed nursing care were 51.17 (24.64), 2.98 (0.51) and 37.71(14.11), respectively. Spearman correlation showed that compassion fatigue was positively associated with emotional labour (p < 0.05), and missed nursing care (p < 0.01). Furthermore, deep acting, a form of emotional labour, had a significant negative relationship with missed nursing care (p < 0.01).ConclusionThe nursing care was rarely missed among intensive care unit nurses, while compassion fatigue and emotional labour were positively associated with missed nursing care. Therefore, focusing on developing interventions for alleviating compassion fatigue and emotional labour may contribute to decrease the incidence of missed nursing care.ImpactThe emotional state of intensive care unit nurses can have a significant impact on the quality of nursing care. It is recommended that nursing managers should promote relevant training in deep acting and instruct nurses to manage emotions during patient interactions, to decrease missed nursing care.Patient or Public ContributionNo patient or public contribution.
Targeted Digital Health Intervention in End‐of‐Life and Hospice Care: A Scoping Review
Authors: Jeon Misun , Jeon Heejung , Kim Sanghee 
Published: January 2025

ABSTRACTAimDigital health interventions, including health analytics, telehealth, mHealth and digitised healthcare systems, are rapidly advancing and demonstrate effectiveness in palliative care. Although end‐of‐life (EOL) and hospice care are within palliative care, they differ in outcomes, target populations and delivery systems. This review examines research trends to guide digital health strategies for EOL and hospice care.DesignScoping review.Data SourcesSystematic searches in CINAHL, MEDLINE, SCOPUS, EMBASE, Cochrane and Web of Science identified studies from 2019 to 2023 using keywords ‘end of life’, ‘hospice’ and ‘digital health’.MethodsFollowing the Joanna Briggs Institute framework, two reviewers independently screened studies, extracted data and categorised health challenges and digital health types per World Health Organization and Deloitte classifications.ResultsAmong 4342 studies, 38 met the inclusion criteria. Most were retrospective (36.8%) without control groups (68.3%). Key targets included healthcare systems (44.2%) and patients (25.6%), focusing on health analytics (44.7%) and mHealth (23.7%) for EOL transitions and symptom management. Main challenges included utilisation (34.9%), efficiency (32.6%) and quality (30.2%).ConclusionDigital health interventions hold potential for enhancing EOL and hospice care but face challenges such as study design limitations, appropriate modality selection, rapport‐building and risks of exacerbating health inequalities. Future interventions should emphasise human‐centred digital capabilities for healthcare providers and user‐centred designs.ImpactThis review highlights opportunities for digital health to improve quality of life for EOL and hospice patients and caregivers. The insights provide guidance for applying digital health interventions in different settings and highlight the importance of equipping healthcare providers with human‐centred digital competencies.Reporting MethodThe reporting was guided by the PRISMA extension for scoping reviews (PRISMA‐ScR).Patient or Public ContributionNo patient or public contribution.
Growth and mechanical correlations of calcified cartilage in Batoidea: A histomorphological study using the Raja asterias model
Authors: Pazzaglia Ugo E. , Reguzzoni Marcella , Terova Genciana , Serena Fabrizio , Mancusi Cecilia , Zarattini Guido , Zecca Piero A. 
Published: January 2025

AbstractThis study investigates the growth and calcification of the appendicular skeleton in Raja asterias (Delaroche, 1809), a member of the Batoidea, to explore the relationship between histomorphology and the mechanics of batoid locomotion within the water column. Although much prior research has focused on the “tessellated pattern” in these fishes, the variable structure of the appendicular skeleton provides fresh insights into the understudied interplay between skeletal histomorphology and the mechanical functions of Batoidea fins. The shape and initial growth of fin cartilage are influenced by the orientation of chondrocyte mitoses prior to mineral deposition, with subsequent calcification playing a pivotal role in shaping skeletal architecture. This study documents two distinct growth patterns: “crustal” and “catenated.” The crustal pattern is predominantly observed in larger skeletal elements, such as the central body structures (skull, rostrum, and jaws), girdles, pterygia, and compound radials, whereas fin radials follow the catenated growth pattern. Notably, early‐stage chondrichthyan cartilage shares similarities with mammalian metaphyseal growth plate cartilage, though in chondrichthyans, the calcified matrix is not resorbed or replaced by bone. Additionally, a previously unrecognized calcification pattern is identified in the pelvic‐fin radials of R. asterias, indicating that the mechanical demands of locomotion in the water column may have driven the evolution of variable fin flexibility in Batoidea. This flexibility is achieved through joint mobility (diarthroses and amphiarthroses), specialized fin structures, and the distinct calcification patterns of the pectoral and pelvic fins.