bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Neutrophil Gelatinase-Associated Lipocalin: A New Biomarker in Asthma-COPD Overlap Syndrome
Authors: Duru Serap , UÇAR FATMA , Kurt Emine Bahar 
Published: February 2021

Abstract
AIM

Human neutrophil gelatinase-associated lipocalin (NGAL) is a protein considered as a noninvasive prognostic biomarker in Asthma-COPD overlap syndrome (ACO). In this study we aimed to show the usability of NGAL as a biomarker in ACO characterized with airway inflammation.
MATERIAL-METHOD:

Three patient groups (Group I: COPD, n: 50, Group II: Asthma, n:50, Group III: ACO, n:50) and a healhty non smoker group (Group IV, n:50) are taken into study. The serum NGAL levels of groups are compared. In addition, in Group I,II,III the level of serum NGAL is compared with the age, duration of smoking (pocket/year), number of attack per year, respiratory function values, serum C reaktive protein (CRP, mg/dL), serum IgE (IU/mL), serum neutrophil (%) and serum lymphocyte (%), blood neutrophil-to-lymphocyte rate and atopy.
FINDINGS:

In patients having ACO when the maximum level of serum exist, it is observed that serum NGAL level is increased as the number of attacks is increased (p = 0.002). On the other hand in the patients having ACO there was also positive correlation between NLR and serum NGAL level (p < 0.05). There was no other relation found among the other variables.
CONCULISION:

In the chronic inflamatuar lung diseases the findings of increase in serum NGAL levels while the severity of the disease suggest us serum NGAL levels might be used as a biomarker for evaluating the inflammation.
Comparison of The Periodontal Status in the Patients with Active and Inactive Rheumatoid Arthritis
Authors: Owlia Fatemeh , Sohanian Shabnam , Karimian Elnaz , Sadrabad Maryam Jalili 
Published: February 2021

Abstract
Introduction: Rheumatoid Arthritis (RA) is the most common chronic inflammatory joint disease. Periodontitis is also an inflammatory disease that affects the periodontal tissue. The former studies have been suggested probable relationship between them. Objectives: The purpose of this study was to evaluate the relation of severity of Periodontitis and RA activity. Materials and Methods: In this study 50 patients who referred to the Rheumatology Department of the Khatamolanbia clinic of Yazd considering inclusion criteria were enrolled in the study. After obtaining the informed consent, based on rheumatologic criteria such as Sedimentation (ESR and Clinical features they were divided into two groups; active and inactive RA. Topics were compared based on age, sex, Rheumatoid Factor, Erythrocyte Sedimentation Rate, Hemoglobin Level for RA Bleeding on Probing, Clinical Attachment Loss, Pocket Depth, and Tooth Loss for periodontitis. Results: The results of this study showed that there was no significant relationship between the variables studied in the active and inactive RA subgroups (p> 0.05). Conclusion: According to the present study while all RA patients show some degree of periodontitis, the periodontitis severity is not correlated with RA activity.
Orbital Edelstein effect in topological insulators
Authors: Osumi Ken , Zhang Tiantian , Murakami Shuichi 
Published: February 2021

Abstract
We theoretically propose a gigantic orbital Edelstein effect in topological insulators and interpret the results in terms of topological surface currents. We numerically calculate the orbital Edelstein effect for a model of a three-dimensional Chern insulator as an example. Furthermore, we calculate the orbital Edelstein effect as a surface quantity using a surface Hamiltonian of a topological insulator, and numerically show that it well describes the results by direct numerical calculation. We find that the orbital Edelstein effect depends on the local crystal structure of the surface, which shows that the orbital Edelstein effect cannot be defined as a bulk quantity. We propose that Chern insulators and Z2 topological insulators can be a platform with a large orbital Edelstein effect because current flows only along the surface. We also propose candidate topological insulators for this effect. As a result, the orbital magnetization as a response to the current is much larger in topological insulators than that in metals by many orders of magnitude.
Synthetic hydrogel nanoparticles for sepsis therapy
Authors: Shea Kenneth , Koide Hiroyuki , Okishima Anna , Hoshino Yu , Kamon Yuri , Yoshimatsu Keiichi , Saito Kazuhiro , Yamauchi Ikumi , Ariizumi Saki , Oku Naoto , Asai Tomohiro , Zhou Yuqi , Xiao Ting-Hui , Goda Keisuke Goda Keisuke 
Published: February 2021

Abstract
Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therapeutic agent for late-stage sepsis. The HNP captures and neutralizes all variants of histones, a major inflammatory mediator released during sepsis. The highly optimized HNP has high capacity and long-term circulation capability for the selective sequestration and neutralization of histones. Intravenous injection of the HNP protected mice against a lethal dose of histones through the inhibition of platelet migration into the lungs. In vivo administration in murine sepsis model mice resulted in near complete survival. These results establish the potential for synthetic, nonbiological polymer hydrogel sequestrants as a new intervention strategy for sepsis therapy and adds to our understanding of the importance of histones to this condition.
Effectiveness of Deep Hyperthermia Combined with Dynamic Traction in the Treatment of Knee Osteoarthritis
Authors: Wang Lichun , Lv Liu , Zhang Hongli , Chen Hua , Li Fei , Zhao Jianyong , Yao Kaixuan , Feng Xiaochen 
Published: February 2021

Abstract
Background: Different treatments with varying clinical efficacies have been applied to treat knee osteoarthritis (KOA). This study observes the effectiveness of deep hyperthermia combined with dynamic traction in KOA treatment. Methods: Deep hyperthermia and dynamic traction was administered to 60 patients with KOA. The pretreatment and 20- and 40-day post-treatment visual analog scale (VAS) and Lysholm score, knee joint cavity effusion, synovium thickness, and synovial blood flow signal (by high-frequency ultrasound) of the patients were recorded. The pretreatment and 20- and 40-day post-treatment Lysholm scores were 47.17 ± 5.07, 63.22 ± 3.44, and 81.98 ± 3.30 (P < 0.001), respectively. Results: The pretreatment and 20- and 40-day post-treatment VAS scores were 5.67 ± 0.91, 2.92 ± 0.81, and 0.60 ± 0.72 (P < 0.001), respectively. The pretreatment and 20- and 40-day post-treatment articular effusion was 5.41 ± 0.46 mm, 4.34 ± 0.34 mm, and 2.32 ± 0.32 mm (P < 0.001), respectively. The pretreatment and 20- and 40-day post-treatment synovial thickness was 3.44 ± 0.31 mm, 2.79 ± 0.15 mm, and 2.32 ± 0.32 mm (P < 0.001), respectively. The pretreatment and 20- and 40-day post-treatment grades of synovial blood flow signal exhibited a significant difference (P < 0.001). Conclusion: Deep hyperthermia combined with dynamic traction can significantly improve the clinical symptoms and pain degree in KOA, reduce joint cavity effusion, synovium thickness, and internal blood flow, and the effect improves with time. The changes in soft tissues observed by high-frequency ultrasound are reliable and consistent with clinical signs.
Tumor Suppressor SMAR1 Regulates PKM Alternative Splicing by HDAC6 Mediated Deacetylation of PTBP1
Authors: Choksi Arpankumar , Parulekar Apoorva , Pant Richa , Shah Vibhuti Kumar , Nimma Ramakrishna , Firmal Priyanka , Singh Smriti , Kundu Gopal C. , Shukla Sanjeev , Chattopadhyay Samit 
Published: February 2021

Abstract
Background:Highly proliferating cancer cells exhibit the Warburg effect by regulation of PKM alternative splicing and promoting the expression of PKM2. Majority of the alternative splicing events are known to occur in the nuclear matrix where various MARBPs actively participate in the alternative splicing events. SMAR1, being a MARBP and an important tumor suppressor, is known to regulate the splicing of various cancer-associated genes. This study focuses on the regulation of PKM alternative splicing and inhibition of the Warburg effect by SMAR1.Methods:Immunohistochemistry was performed in breast cancer patient samples to establish the correlation between SMAR1 and PKM isoform expression. Further, expression of PKM isoforms upon modulation in SMAR1 expression in breast cancer cell lines was quantified by qRT-PCR and western blot. The acetylation status of PTBP1 was estimated by immunoprecipitation along with its enrichment on PKM pre-mRNA by CLIP in SMAR1 knockdown conditions. The role of SMAR1 in tumor metabolism and tumorigenesis was explored by in vitro enzymatic assays and functional assays upon SMAR1 knockdown. Besides, in vivo tumor formation by injecting adeno-SMAR1 transduced MDA-MB-231 cells in NOD/SCID mice was performed. Results:The expression profile of SMAR1 and PKM isoforms in breast cancer patients revealed that SMAR1 has an inverse correlation with PKM2 and a positive correlation with PKM1. Further quantitative PKM isoform expression upon modulation in SMAR1 expression also reflects that SMAR1 promotes the expression of PKM1 over tumorigenic isoform PKM2. SMAR1 deacetylates PTBP1 via recruitment of HDAC6 resulting in reduced enrichment of PTBP1 on PKM pre-mRNA. SMAR1 inhibits the Warburg effect, tumorigenic potential of cancer cells and in vivo tumor generation in PKM2 dependent manner.Conclusions:SMAR1 regulates PKM alternative splicing by causing HDAC6 dependent deacetylation of PTBP1, resulting in reduced enrichment of PTBP1 on PKM pre-mRNA. Additionally, SMAR1 suppresses glucose utilization and lactate production via repression of PKM2 expression. This suggests that tumor suppressor SMAR1 inhibits tumor cell metabolism and tumorigenic properties of cancer cells via regulation of PKM alternative splicing.
Serum Metabolic Profiling Identifies a Biomarker Panel for Improvement of Prostate Cancer Diagnosis
Authors: Xu Huan , Chen Junyi , He Jingyi , Ji Jin , Cao Zhi , Chen Xi , Xu Yalong , He Xing , Xu Guowang , Zhou Lina , Wei Xuedong , Hou Jianquan , Wang Zhong , Yang Bo , Wang Fubo 
Published: February 2021

Abstract
Objectives: To identify and validate a biomarker panel by serum metabolic profiling for improvement of PCa diagnosis. Materials and Methods: Totally, 134 individuals were included in this study. Among them, 39 PCa patients and 45 control patients (negative prostate biopsy) were involved in the discovery phase and 50 healthy controls were enrolled for validation phase of metabolomics study. LC-MS Analysis was used for the identification of the serum metabolites of patients. Results: Logistics regression analysis shows that 5 metabolites (dMePE(18:0/18:2), PC(16:0/20:2), PS(15:0/18:2), SM(d16:0/24:1), Carnitine C14:0) were significantly changed in PCa patients compared with control patients. A metabolic panel (MET) was calculated, showing a significantly higher diagnostic performance than PSA in differentiating PCa from control patients (AUC [MET vs. PSA]: 0.823 ± 0.046 vs. 0.712 ± 0.057, p<0.001). Moreover, this panel was superior to PSA in distinguishing PCa from negative prostate biopsies when PSA levels were less than 20ng/ml (AUC [MET vs. PSA]: 0.836 ± 0.050 vs. 0.656 ± 0.067, p<0.001). In the validation set, the MET panel yielded an AUC of 0.823 in distinguishing PCa patients from healthy controls, showing a significant improvement of PCa detection. Conclusions: The metabolite biomarker panel discovered in this study presents a good diagnostic performance for the detection of PCa.
Population Genomics Provides Insights into the Population Structure and Climate-driven Adaptation of Collichthys Lucidus
Authors: Zhao Linlin , Qu Fangyuan , Song Na , Han Zhiqiang , Gao Tianxiang , Zhang Zhaohui 
Published: February 2021

Abstract
Background: Understanding the genetic structure and local adaptive evolutionary mechanisms of marine organisms is crucial for the conservation and management of biological resources. Collichthys lucidus is an ideal candidate for investigating population differentiation and local adaptation under heterogeneous environmental pressure. Results: To elucidate the fine-scale genetic structure and local thermal adaptation of C. lucidus, we performed restriction site-associated DNA tag sequencing (RAD-seq) of 177 individuals from 8 populations, and a total of 184,708 high-quality single nucleotide polymorphisms (SNPs) were identified. All the results revealed significant population structure with high support for two distinct genetic clusters, namely, the northern group (populations DL, TJ, LYG, NT, ZS, and WZ) and southern group (populations XM and ZH). The genetic diversity of the southern group was evidently lower than that of the northern group, which indicated that the southern group was possibly under climate-driven natural selection. In addition, a total of 314 SNPs were found to be significantly associated with temperature variation. Annotations of temperature-related SNPs suggested that genes involved in material (protein, lipid, and carbohydrate) metabolism and immune responses were critical for adaptation to spatially heterogeneous temperatures in natural C. lucidus populations. Conclusion: In the context of anthropogenic activities and environmental change, the results of the present population genomic work could make important contributions to the understanding of genetic differentiation and adaptation to changing environments.
Optimization of Fermentation Conditions for Recombinant Lipase Expression At Small Scale Using Response Surface Methodology for Preliminary Production In Two Bioreactor Platforms.
Authors: López Angela Liliana Meza , Acosta-González Alejandro , Manrique Ingrid Yamile Pulido , Correa Rosa Erlide Prieto , Junca Carlos Jimenez 
Published: February 2021

Abstract
Background: Pseudomonas lipases are widely used in industrial applications due to its unique biochemical properties, but one of the biggest limitations are the low yields obtained in native strains therefore, organisms as E. coli are used for the recombinant lipase overexpression. However, the recombinant lipase is accumulated as inclusion bodies and it affects biological activity, making that researchers evaluate different fermentation conditions to improve the activity of recombinant enzymes. In this study, a statistical experimental design was implemented to evaluate the effects of temperature, agitation rate and osmolyte concentration on the recombinant lipase activity produced in E. coli BL21 (DE3). Once the significant variables were identified, an optimization by a Response Surface Methodology was applied to maximize the lipase production. Results: The Box-Behnken designs revealed different optimal fermentation conditions for each osmolyte experiment. The glycerol showed the highest specific lipase activity compared to the other osmolytes and 0.1 M of osmolyte glycerol,5°C and 110 rpm showed the highest significant increase on the specific lipase activity and the data fitted the model very well. The validation showed that 452.01 U/mg of specific lipase activity was obtained which was significantly higher compared to the group where no glycerol was added (271.38 U/mg). The relative recombinant lipase expression was 2.7-fold lower at 5°C compared to 25 °C, but at 5°C the lipase activity was significantly higher. In addition, when the 3 L shaken Erlenmeyer Bioreactor was used to produce the recombinant lipase based on the power input parameter, the specific lipase activity was not significantly different from that found in Schott (408,4 U/mg and 452 U/mg, respectively), which means that this Bioreactor platform should be used for future scale-up processes.Conclusion: Low temperatures, low agitation rates and 0.1 M of glycerol in the autoinduction media enhanced the activity of the recombinant lipase produced in E. coli BL21(DE3). The optimized conditions and the 3 L shaken Erlenmeyer Bioreactor can be used to produce the recombinant enzyme in a higher volume based on the power input parameter. Further studies using this strategy may lead to the identification of optimal culture conditions for a given recombinant enzyme facilitating the large-scale bioprocess implementation.
Biosynthesis of Silver Nanoparticles and Their Biocontrol Potentials Against Aspergillus Niger and Fusarium Udum
Authors: Sikka Aneesh , Sodimalla Triveni , YALAVARTHI NAGARAJU 
Published: February 2021

Abstract
Silver nanoparticles can be biosynthesized from bacteria, fungi, and plant extracts but due to their ability to synthesize nanoparticles in varying sizes and shapes at ease, bacterial has drawn interest. Bacterial based biosynthesis is effective, inexpensive, and simple thus, Pseudomonas fluorescence cell filtrates were used to synthesize silver nanoparticles in the present study. The chromatic shifts (yellow to brown) in the media after overnight incubation and the absorption of UV-Vis spectra at 420 nm confirmed the biosynthesis of AgNP’s. Besides that, the SPR analysis of AgNP’s showed a 400–500 nm band width, supporting the formation of silver nanoparticles and their small size with a uniform shape. AgNP’s transmission electron microscopy (TEM) images confirmed their shape as quasi spherical, mean size as 30 nm and anisotropy. From the Zeta potential analysis (-42.7 mV at pH = 7 with a single peak), highly repulsive nature of nanoparticles was confirmed. On the other hand, bio-fabricated silver nanoparticles were tested for antifungal activity against Fusarium udum and Aspergillus niger under in vitro conditions. At 150 ppm concentration of AgNP’s, Fusarium udum and Aspergillus niger were inhibited up to 100 and 80.50 %, respectively. In conclusion, synthesis of nanoparticle with aqueous Pseudomonas fluorescence extract is simple and environmentally benign.
Risk Factors for Acute Postoperative Sort Throat (POST) After Supraglottic Device Use&nbsp;
Authors: Huang Yanzhe , Chen Kaizheng , Shen Xia 
Published: February 2021

Abstract
Background. To identify risk factors for acute postoperative sort throat (POST) after general anesthesia using a supraglottic airway device (SAD) in adults undergoing elective tympanoplasty.Methods. The medical records of 1424 adults who underwent elective tympanoplasty under general anesthesia using an SAD were reviewed retrospectively. Patients received one of four SADs at the discretion of the anesthesiologists: flexible reinforced LMA (FLMA), Ambu AuraFlex FLMA, or two local brand devices (Tuoren FLMA or Tuoren Esophageal Drainage LMA). POST upon discharge from the postanesthesia care unit (PACU) was measured using a visual analog sale (VAS) and categorized as no pain, mild pain, and moderate to severe pain. Data regarding potential risk factors for POST were collected from the medical records. Results. The mean patient age was 43.24 years; 622 patients were male and 802 were female. The overall incidence of POST during the PACU stay was 38.1%. Female sex, certain types of SAD, higher intracuff pressure, and longer duration of surgery were independent risk factors for POST on multivariate logistic regression analysis. Compared to FLMA, both the Tuoren FLMA and the Tuoren Esophageal Drainage LMA were risk factors for POST. The Tuoren Esophageal Drainage LMA was the strongest independent risk factor for moderate to severe POST.Conclusions. Use of local brand SADs contributed to the development of POST after elective tympanoplasty under general anesthesia. The Tuoren Esophageal Drainage LMA was the strongest predictor of moderate to severe POST.
The Application of Ozonated Water Rearranges the Transcriptome of the Vitis Vinifera L. Fruit
Authors: Campayo Ana , Savoi Stefania , Romieu Charles , López-Jiménez Alberto José , Hoz Kortes Serrano de la , Salinas Rosario , Torregrosa Laurent , Alonso Gonzalo 
Published: February 2021

Abstract
Ozonated water has become an innovative, environmentally friendly tool for controlling the development of fungal diseases in the vineyard or during grape postharvest conservation. However, little information is currently available on the effects of ozonated water sprayings on the grapevine physiology. Using the microvine model, we studied the transcriptomic response of leaf and fruit organs to this treatment. The response to ozone was observed to be organ and developmental stage-dependent, with a decrease of the number of DEGs (differentially expressed genes) in the fruit from the onset of ripening to later stages. The most highly up-regulated gene families were heat-shock proteins and chaperones. Other up-regulated genes were involved in oxidative stress homeostasis such as those of the ascorbate-glutathione cycle and glutathione S-transferases. In contrast, genes related to cell wall development and secondary metabolites (carotenoids, terpenoids, phenylpropanoids / flavonoids) were generally down-regulated after ozone treatment, mainly in the early stage of fruit ripening. This down-regulation may indicate a possible carbon competition favouring the re-establishment and maintenance of the redox homeostasis rather than the synthesis of secondary metabolites at the beginning of ripening, the most ozone responsive developmental stage.
AntiTNF-alpha Therapy Normalizes Levels of Lipids and Adipokines in Psoriatic Patients in the Real-life Settings
Authors: Olejniczak-Staruch Irmina , Narbutt Joanna , Ceryn Justyna , Skibinska Malgorzata , Bednarski Igor , Woźniacka Anna , Sieniawska Joanna , Kraska-Gacka Marzena , Śmigielski Janusz , Ciążyńska Magdalena , Noweta Marcin , Waszczykowski Michał , Owczarek Witold , Reich Adam , Lesiak Aleksandra 
Published: February 2021

Abstract
Studies have shown that the levels of pro-inflammatory adipokines in patients with psoriasis are higher than in general population. The aim of the study was to investigate the influence of 36-month therapy with TNF-α inhibitors (adalimumab, etanercept, infliximab) on the levels of adipokines (resistin, adiponectin, leptin) and lipids (TG, cholesterol, LDL, HDL) in 37 psoriasis patients and 30 healthy controls. The mean serum concentrations of adiponectin in patients from adalimumab, etanercept and infliximab group were similar to control group (p > 0.05, 142.71, 164.32, 129.35 and 174.44 µg/ml respectively). Resistin levels were higher in patients (p < 0.05, 4.48, 4.53 and 3.39 ng/ml respectively) than in controls (3.05 ng/ml). Mean leptin concentrations were significantly higher (p < 0.05) in the study group than in subjects without psoriasis (428.61, 523,24, 755,27 and 154.10 pg/ml respectively). A significant decrease in the mean resistin concentration was observed under the influence of biological therapy (p < 0.05). Decrease in serum leptin level was noted in etanercept and infliximab groups (p = 0.001 and p = 0.002 respectively). Improvement in all lipidogram parameters was noted in all examined groups (p < 0.05). Results may prove that biologic therapy affects the systemic inflammation associated with psoriasis and this effect persists with long-term therapy.