bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA LOC100507144 acts as a novel regulator of CD44/Nanog/Sox2/miR‐302/miR‐21 axis in colorectal cancer
Authors: Ebrahimi Nasim , Rezanejad Hajar , Asadi Malek Hossein , Vallian Sadeq 
Published: December 2021

AbstractLong noncoding RNAs (lncRNAs) appear as vital regulators and biomarkers in many human cancers. LOC100507144 is a validated lncRNA located in the neighborhood of CD44 in a head‐to‐head configuration, and its expression and function in cancer cells are still unknown. This research aimed to find out more about the expression and function of this lncRNA in colorectal cancer (CRC). Our expression data represented that the expression of LOC100507144 transcript was substantially higher in tumors with advanced stages, lymph node metastasis, and vascular invasion. Loss‐of‐function examinations demonstrated that LOC100507144 contributed to CRC cell proliferation by restricting apoptosis, cellular senescence, and promoting cell cycle. Gain‐of‐function experiments also confirmed these results. Our data illustrated that LOC100507144 enhanced the migration and the epithelial to mesenchymal transition (EMT) of CRC cells, accompanied by the generation of cells with stemness characteristics. Our findings revealed that the knocking‐down of LOC100507144 inhibited the expression of crucial stemness factors, including CD44, Nanog, and Sox2, and accordingly resulted in suppressing their targets, miR‐302 and miR‐21. Overall, the current study's findings for the first time reveal that LOC100507144 could enhance CRC progression and metastasis through regulation of the CD44/Nanog/Sox2/miR‐302/miR‐21 axis.
Does neural face processing explain effects of an attachment‐based intervention on maternal sensitivity? A randomized controlled study including pre‐ and postintervention measures
Authors: Kolijn Laura , van den Bulk Bianca G. , Euser Saskia , Bakermans‐Kranenburg Marian J. , van IJzendoorn Marinus H. , Huffmeijer Rens 
Published: December 2021

AbstractBackgroundAlthough there is a large body of literature highlighting the behavioral effects of parenting interventions, studies on the neurocognitive mechanisms involved in such intervention effects remain scarce.PurposeThe aim of the current study was to test whether changes in neural face processing (as reflected in N170 amplitudes) would act as a mediator in the association between the Video‐feedback Intervention to promote Positive Parenting and Sensitive Discipline (VIPP‐SD) and maternal sensitivity.MethodsA total of 66 mothers of whom a random 33% received the VIPP‐SD and the others a “dummy” intervention participated in pre‐ and postintervention assessments. We recorded mothers' electroencephalographic (EEG) activity in response to photographs of children's neutral, happy, and angry facial expressions. Maternal sensitivity was observed while mothers interacted with their offspring in a semi‐structured play situation.ResultsIn contrast with our expectations, we did not find evidence for mediation of intervention effects on maternal sensitivity by the N170.ConclusionWe discuss that parenting support programs may yield different effects on neurocognitive processes depending on the population and provide recommendations for future research. Our study underscores the importance of reporting null findings and preregistering studies in the field of neurocognitive research.
Comparison of L5 pedicle morphology in patients with and without L5 spondylolysis
Authors: Baker Joseph F. 
Published: December 2021

AbstractOnly a small number of studies have reported on L5 pedicle morphology in the presence of spondylolisthesis let alone isolated spondylolysis and findings are therefore variable. The aim of this radiographic study was to analyze L5 pedicle morphology in the presence and absence of L5 spondylolysis. The was a retrospective cross‐sectional analysis of computed tomographic (CT) imaging. Assessment of the L5 pedicle morphology (height, width, length, transverse angle and screw length) were measured in patients with and without L5 spondylolysis. Pelvic measures including pelvic incidence, sacral anatomic orientation and sacral table angle were recorded. Patients were matched for age and gender. Twenty‐three patients with spondylolysis were matched to 46 patients without. The presence of spondylolysis alone did not have a significant influence on either pelvic or pedicle morphologic parameters. Only with the presence of associated spondylolisthesis was there a difference noted with an increase in pedicle length observed. Correlation analysis suggested further morphologic changes may result with increased remodeling. Isolated spondylolysis at L5 appears to have little influence on pedicle morphology in this CT‐based analysis. Morphologic changes appear likely to become significant only with associated spondylolisthesis and associated remodeling.
Evaluation of Geum urbanum L. Extracts with Respect to Their Antimicrobial Potential
Authors: Bunse Marek , Mailänder Lilo K. , Lorenz Peter , Stintzing Florian C. , Kammerer Dietmar R. 
Published: December 2021

AbstractPreparations derived from roots and rhizomes of Geum urbanum L. are traditionally used for the treatment of ulcers and irritations of mucous membranes of the mouth, stomach, and intestinal tract. In complementary medicine, fermentation is one of the methods applied to recover plant extracts used for the production of such pharmaceutical preparations. The present study was performed to characterize the secondary metabolites and to evaluate the antimicrobial potential of different G. urbanum root and rhizome extracts. For this purpose, individual metabolites of fresh and fermented G. urbanum root and rhizome extracts were analyzed by HPLC‐DAD‐MSn and GC/MS. Among others, rare ellagitannin‐sulfates could be characterized by LC/MSn. In addition, the antibacterial activity of various extracts of fresh and dried G. urbanum roots and rhizomes against Staphylococcus aureus (ATCC 6538) and Cutibacterium acnes (CP033842.1; FDAARGOS 503 chromosome) were assessed and compared to that of G. rivale. Furthermore, low‐ and high‐molecular tannins were fractionated by column chromatography, demonstrating the latter to exhibit highest antibacterial activity.
Adhesion and proliferation properties of type I collagen‐derived peptide for possible use in skin tissue engineering application
Authors: Sivaraman K. , Muthukumar K. , Shanthi C. 
Published: December 2021

AbstractThe surface properties of three‐dimensional scaffolds are improved by coating or covalently linking certain adhesion‐promoting proteins or peptides. In the present study, the effect of type I collagen‐derived peptide (GKNGDDGEA) on adhesion and proliferation of HaCaT keratinocytes and NIH3T3 murine fibroblast cell lines was studied to assess its suitability for possible skin tissue engineering applications. Cell adhesion and proliferation of HaCaT and NIH3T3 were found to be enhanced by peptide coating. The optimum peptide coating densities to obtain the best cell adhesion and proliferation were found to be 0.827 µmoles/cm2 and 0.62 µmoles/cm2 for HaCaT and NIH3T3, respectively. Cell adhesion, in the presence of anti‐integrin α1 antibody, inhibited attachment of NIH3T3 cells indicating the involvement of integrin α1 receptor. However, the attachment of HaCaT cells was not affected by anti‐integrin treatment. The higher expression of paxillin confirmed the effect of the peptide in mediating focal adhesion kinases (FAKs) in cell adhesion and proliferation. Gene expression analysis was performed on cell migration proteins like Rho, Rac, Cdc42, integrin receptor α1, and β1, and the extracellular matrix modulating proteins like MMP2, TIMP, and COL1A1 to validate their role on the peptide‐mediated cell proliferation. Immunofluorescence analysis showed the distribution and localisation of phospho‐FAK on cells cultured on the peptide‐coated surfaces. Results support the role of peptides in enhancing cell adhesion and proliferation properties.
Melatonin protects H9c2 cardiomyoblasts from oxygen‐glucose deprivation and reperfusion‐induced injury by inhibiting Rac1/JNK/Foxo3a/Bim signaling pathway
Authors: Wang Yulin , Jian Ying , Zhang Xiaofu , Ni Bin , Wang Mingwei , Pan Chunqi 
Published: December 2021

AbstractMelatonin has been shown to protect against ischemia/reperfusion (I/R)‐induced myocardial injury, however, the precise molecular mechanisms have not been fully clarified. The present study was aimed to investigate whether inactivation of Rac1/JNK/Foxo3a/Bim signaling pathway is responsible for the protective effect of melatonin on I/R‐induced myocardial injury. Our results showed that Foxo3a downregulation contributed to the protective effect of melatonin on OGD/R‐induced injury of H9c2 cardiomyoblasts. Melatonin treatment led to a reduced activity of Rac1, which was responsible for Foxo3a downregulation and decreased cell injury in OGD/R‐exposed H9c2 cells. Furthermore, JNK acts as a downstream effector of Rac1 in mediating melatonin‐induced inactivation of Foxo3a/Bim signaling pathway and decreased cell injury in OGD/R‐exposed H9c2 cells. In conclusion, our results indicate that melatonin protects H9c2 cells against OGD/R‐induced injury by inactivating the Rac1/JNK/Foxo3a/Bim signaling pathway. This study provided a novel insight into the protective mechanism of melatonin against I/R‐induced myocardial injury.
P2X7 receptor inhibition attenuates podocyte injury by oxLDL through deregulating CXCL16
Authors: Zhu Yanji , Li Qian , Chen Yuan , Tian Minle , Xun Wenlong , Sun Shuzhen 
Published: December 2021

AbstractThis study aims to evaluate the effect of purinergic ligand‐gated ion channel 7 receptor (P2X7R) antagonist A438079 in kidneys of children with primary nephrotic syndrome (PNS). In vitro, human podocytes were respectively stimulated with oxLDL (80 µg/ml), A438079 (10 µmol/L), or the compound oxLDL and A438079 together. CXC chemokine ligand 16 (CXCL16) and P2X7R expression levels were detected by Western blot and immunofluorescence assay, respectively. Immunofluorescence assay was used to detect Dil‐oxLDL, and a Colorimetric Cholesterol Detection Kit was used for quantitative determination. Our results demonstrated that CXCL16 and P2X7R expression levels were remarkably increased in the renal tissue from children with PNS, particularly in the same location. Furthermore, in contrast to children with minimal change disease, the expressions of P2X7R and CXCL16 in renal tissue of children with focal segmental glomerulosclerosis were more obvious. In vitro, CXCL16 and P2X7R expression levels in human podocytes stimulated with oxLDL were markedly elevated accompanying higher intracellular lipid accumulation compared with the normal control group. In addition, pretreatment of human podocytes with A438079 before the start of oxLDL stimulation causes a significant reduction in CXCL16 expression and a decrease in lipid accumulation. Overall, CXCL16 and P2X7R may participate in the progression of PNS. The lipid accumulation reduction caused by A438079 may be through deregulating the CXCL16 pathway, suggesting that there is a potential role for P2X7R antagonists to remedy PNS.
Low‐frequency oscillations of murine skin microcirculations and periodic changes of [Ca2+]i and [NO]i levels in murine endotheliocytes: An effect of provocative tests
Authors: Serov Dmitriy , Tankanag Arina , Astashev Maksim 
Published: December 2021

AbstractThe five frequency intervals of skin blood oscillation were described: cardiac, respiratory, myogenic, neurogenic, and endothelial. The endothelial interval is derived into NO‐independent and NO‐dependent. The exact molecular, cell, or systemic mechanisms of endothelial oscillations generation are unclear. We proposed that oscillations of Ca2+ and NO in endotheliocytes may be possible sources of skin blood perfusion (SBP) oscillations in endothelial interval. To examine our hypothesis we compared the oscillations of cytoplasmic Ca2+ and NO ([Ca2+]i and [NO]i) concentration in cultured murine microvascular endotheliocytes and SBP oscillations in mice. Local heating test and model hypoxia were used as tools to evaluate an interconnection of studied parameters. [Ca2+]i and [NO]i were measured simultaneously by Fura‐2 AM and DAF‐FM. The SBP was measured by laser Doppler flowmetry. The [Ca2+]i and [NO]i oscillations at 0.005−0.01 Hz were observed in endotheliocytes, that coincides the ranges of NO‐independent endothelial interval. Heating decreased amplitude of [Ca2+]i and [NO]i oscillations in cells in NO‐independent endothelial interval, while amplitudes of SBP oscillations increased in NO‐independent and NO‐dependent intervals. Hypoxia reduced the [NO]i oscillations amplitude. Heating test during hypoxia increased NO‐independent endothelial SBP oscillations and decreased myogenic ones, did not effect on [NO]i oscillations, and shifted [Ca2+]i oscillations peak from 0.005−0.01 Hz to 0.01−0.018 Hz. We observed the [Ca2+]i and [NO]i oscillations synchronization within a cell and between cells for the first time. Heating abolished these synchronizations. Therefore low‐frequency [Ca2+]i and [NO]i oscillations in endotheliocytes may be considered as modulators of low‐frequency endothelial SBP oscillations.
DNA Electrostatics: From Theory to Application
Authors: Jambrec Daliborka , Gebala Magdalena 
Published: December 2021

AbstractNumerous DNA biosensor platforms developed in the last decades rely on DNA electrostatics as the basis for detection. However, the overwhelming number of theoretical studies and computational models of DNA electrostatics poses a barrier to leveraging our deeper mechanistic understanding in this area for the development of new technologies that will push the field towards more sensitive, quantitative, and reliable DNA‐based sensors. In this review, we will bridge the gap between the theory and applications of DNA charge and electrical surfaces to overcome this barrier. We will discuss key theories such as Manning's counterion condensation theory, the Poisson‐Boltzmann equation, and the Gouy‐Chapman model, and provide examples of technological applications that rely on these theories.
Calixarenes Incorporating Sulfonamide Moieties: Versatile Ligands for Carbonic Anhydrases Inhibition
Authors: Sbravati Davide , Bonardi Alessandro , Bua Silvia , Angeli Andrea , Ferraroni Marta , Nocentini Alessio , Casnati Alessandro , Gratteri Paola , Sansone Francesco , Supuran Claudiu T. 
Published: December 2021

AbstractCarbonic anhydrases (CAs) continue to represent a relevant pharmaceutical target. The need of selective inhibitors and the involvement of these metalloenzymes in many multifaceted diseases boost the search for new ligands able to distinguish among the different CA isoforms, and for multifunctional systems simultaneously able to inhibit CAs and to interfere with other pathological events by interacting with additional targets. In this work, we successfully explored the possibility of preparing new CAs ligands by combining calixarenes with benzensulfonamide units. Inhibition tests towards three human CA isoforms evidenced, for some of the ligands, Ki values in the nanomolar range and promising selectivity. X‐ray and molecular modeling studies provided information on the mode of binding of these calixarene derivatives. Thanks to the encouraging results and the structural features typical of the calixarene scaffold, it is then possible to plan for the future the design of multifunctional inhibitors for this class of widely spread enzymes.
Reaching New Biocatalytic Reactivity Using Continuous Flow Reactors
Authors: Cosgrove Sebastian C. , Mattey Ashley P. 
Published: December 2021

AbstractThe use of flow reactors in biocatalysis has increased significantly in recent years. Chemists have begun to design flow systems that even allow new biocatalytic reactions to take place. This concept article will focus on the design of flow systems that have allowed enzymes to go beyond their limits in batch. The case is made for moving towards fully continuous systems. With flow chemistry increasingly seen as an enabling technology for automated synthesis, and with advancements in AI‐assisted enzyme design, there is a real possibility to fully automate the development and implementation of a continuous biocatalytic processes. This will lead to significantly improved enzyme processes for synthesis.
Hydrogen‐Bridged Oligosilanylsilyl Mono‐ and Oligosilanylsilyl Dications
Authors: Nimoth Jelte P. , Müller Thomas 
Published: December 2021

AbstractHydrogen‐bridged oligosilanylsilyl borates 8 [B(C6F5)4], 9[B(C6F5)4] and diborates 10 [B(C6F5)4]2 have been prepared by hydride transfer between α‐ω‐dihydrido‐ (11) and branched tetrahydrido‐oligosilanes (13) and trityl cation. The obtained cyclic intramolecularly stabilized silylium ions 8, 9 and bissilylium ion 10 were characterized by low temperature NMR spectroscopy supported by the results of density functional calculations. The branched Si−H−Si monocation 9 undergoes at low temperatures a fast degenerate rearrangement, which exchanges the Si−H groups with a barrier of 31 kJ mol−1 via an antarafacial transition state. Reaction of the branched monocation 9 with a second equivalent of trityl cation or of the branched oligosilane 13 with two equivalents of trityl cation, gives at −80 °C the corresponding bissilylium ion 10, an example for a new class of highly reactive poly‐Lewis acids.
Add a Pinch of Tetrel: The Transformation of a Centrosymmetric Metal into a Nonsymmorphic and Chiral Semiconductor
Authors: Lee Shannon J. , Viswanathan Gayatri , Carnahan Scott L. , Harmer Colin P. , Akopov Georgiy , Rossini Aaron J. , Miller Gordon J. , Kovnir Kirill 
Published: December 2021

AbstractCentrosymmetric skutterudite RhP3 was converted to a nonsymmorphic and chiral compound RhSi0.3P2.7 (space group P212121) by means of partial replacement of Si for P. The structure, determined by a combination of X‐ray crystallography and solid state 31P NMR, exhibits branched polyanionic P/Si chains that are unique among metal phosphides. A driving force to stabilize the locally noncentrosymmetric cis‐RhSi2P4 and fac‐RhSi3P3 fragments is π‐electron back‐donation between the Rh t2g‐type orbitals and the unoccupied antibonding Si/P orbitals, which is more effective for Si than for P. In situ studies and total energy calculations revealed the metastable nature of RhSi0.3P2.7. Electronic structure calculations predicted centrosymmetric cubic RhP3 to be metallic which was confirmed by transport properties measurements. In contrast, the electronic structure for chiral orthorhombic RhSi0.3P2.7 contained a bandgap, and this compound was shown to be a narrow gap semiconductor.
Drug‐Derived Surface‐Active Ionic Liquids: A Cost‐Effective Way To Expressively Increase the Blood‐Stage Antimalarial Activity of Primaquine
Authors: Silva Ana Teresa , Oliveira Isabel S. , Gomes Joana , Aguiar Luísa , Fontinha Diana , Duarte Denise , Nogueira Fátima , Prudêncio Miguel , Marques Eduardo F. , Teixeira Cátia , Ferraz Ricardo , Gomes Paula 
Published: December 2021

AbstractInspired by previous disclosure of room‐temperature ionic liquids derived from primaquine and cinnamic acids, which displayed slightly enhanced blood‐stage activity compared to the parent drug, we have now combined this emblematic antimalarial with natural fatty acids. This affords surface‐active ionic liquids whose liver‐stage antiplasmodial activity is either retained or slightly enhanced, while revealing blood‐stage antiplasmodial activity at least one order of magnitude higher than that of the parent compound. These findings open new perspectives towards the cost‐effective recycling of classical drugs that are either shelved or in decline, and which is not limited to antimalarial agents.
Blockchain based choreographies: The construction industry case study
Authors: Spalazzi Luca , Spegni Francesco , Corneli Alessandra , Naticchia Berardo 
Published: December 2021

AbstractBPMN choreography is a modeling language capable to describe scenarios where several independent participants have to collaborate in a climate of opposing interests and therefore are forced to trust each other. For this reason, in many contexts, a strong need for transparency, responsibility, and choreography compliance arise by the various participants. Blockchains and smart contracts, thanks to their characteristic of providing a decentralized and consensus‐based validation mechanism, seem to be able to meet these needs in an untrusted scenario. Nevertheless, most of the related work focused either on transparency, accountability, or compliance, but none on all three of them. Furthermore, such works do not take into account the nondeterministc nature of choreographies. This work aims at using blockchains and smart contracts in this scenario providing a formally well‐defined set of tools to match all three the aforementioned requirements. This work applies the proposed techniques to a case study from the construction industry, an economical relevant application domain where the demand for transparency, accountability, and compliance with procurement contracts (that can be modeled as choreographies) is very strong.
Homogeneous Water Oxidation Catalyzed by First‐Row Transition Metal Complexes: Unveiling the Relationship between Turnover Frequency and Reaction Overpotential
Authors: Hsu Wan‐Chi , Wang Yu‐Heng 
Published: December 2021

AbstractThe utilization of earth‐abundant low‐toxicity metal ions in the construction of highly active and efficient molecular catalysts promoting the water oxidation reaction is important for developing a sustainable artificial energy cycle. However, the kinetic and thermodynamic properties of the currently available molecular water oxidation catalysts (MWOCs) have not been comprehensively investigated. This Review summarizes the current status of MWOCs based on first‐row transition metals in terms of their turnover frequency (TOF, a kinetic property) and overpotential (η, a thermodynamic property) and uses the relationship between log(TOF) and η to assess catalytic performance. Furthermore, the effects of the same ligand classes on these MWOCs are discussed in terms of TOF and η, and vice versa. The collective analysis of these relationships provides a metric for the direct comparison of catalyst systems and identifying factors crucial for catalyst design.