bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiling Serum Antibodies with a Pan Allergen Phage Library Identifies Key Wheat Allergy Epitopes
Authors: Monaco Daniel R. , Sie Brandon M. , Nirschl Thomas R. , Knight Audrey C. , Sampson Hugh A. , Nowak-Wegrzyn Anna , Wood Robert A. , Hamilton Robert G. , Frischmeyer-Guerrerio Pamela A. , Larman H. Benjamin 
Published: May 2021

Abstract
Allergic reactions occur when IgE molecules become crosslinked by antigens such food proteins. The physiologic consequences range from mild to life-threatening. Here we create the ‘AllerScan’ bacteriophage display library for efficiently profiling allergic antibodies to characterize binding specificities associated with known allergens. AllerScan enables sensitive and unbiased characterization of circulating IgE and IgG antibody reactivities against thousands of allergenic proteins from hundreds of organisms at peptide resolution. Using AllerScan to analyze food allergy reactivities, we identify robust anti-wheat IgE reactivities in wheat allergic patients but not in wheat-sensitized individuals. Meanwhile, a wheat allergen epitope, alpha purothionin, elicits dominant IgE responses among allergic patients but frequent IgG responses among sensitized and non-allergic patients; a double-blind placebo-controlled trial shows that alpha purothionin reactivity, among others, is strongly modulated by oral immunotherapy. Our proof-of-principle results thus suggest that AllerScan to be a high-throughput platform for unbiased analysis of allergic antibody fine specificities.
Chemically Defined and Xeno-free Culturing Protocol for Human Extended Pluripotent Stem Cells
Authors: Liu Bei , Chen Shi , Xu Yaxing , Lyu Yulin , Bai Yun , Xu Jun , Deng Hongkui 
Published: May 2021

Abstract
Human extended pluripotent stem (EPS) cells have shown great applicative potentials in basic and translational research. One prerequisite for the wide applications of EPS cells is the development of a xeno-free culture system for maintain these cells in vitro. Here we report a protocol for culturing and generating human EPS cells using a chemically defined and xeno-free culture system.
Agent-based Multi-tier SLA Negotiation for Intercloud
Authors: Li Lin , Lin Kaibiao , Zhu Shunzhi 
Published: May 2021

Abstract
The evolving intercloud enables idle resources to be traded among cloud providers to facilitate optimizing utilization and to improve the cost-effectiveness of service for cloud consumers. However, several challenges are raised for this multi-tier dynamic market, where cloud providers not only compete for consumer requests but also cooperate with each other. To establish a healthier and more efficient intercloud ecosystem, this paper proposed a multi-tier agent-based fuzzy constraint-directed negotiation (AFCN) model for a fully distributed negotiation environment without a broker to coordinate the negotiation process. The novelty of AFCN is the use of a fuzzy membership function to represent imprecise preferences of the agent, which not only reveals the opponent’s behavior preference but can also specify the possibilities prescribing the extent to which the feasible solutions are suitable for the agent’s behavior. Moreover, this information can pass and guide each tier of negotiation to generate a more favorable proposal. Thus, the multi-tier AFCN can not only improve the performance of negotiation, but also enforce global consistency to improve the integrated solution capacity in the intercloud. The experimental results demonstrate that the proposed multi-tier AFCN model outperforms other agent negotiation models and gives full play to the efficiency and scalability of the intercloud in terms of the level of satisfaction, the ratio of successful negotiation, the average revenue of the cloud provider, and the buying price of the unit cloud resource.
Knowledge, attitudes and practices among Lebanese Obstetricians and gynecologists toward Coronavirus Disease-2019 (COVID-19) and pregnancy
Authors: Youssef Dalal , Abass Linda Abou , Berry Atika , Youssef Janet 
Published: May 2021

Abstract
Introduction: The pandemic of Coronavirus Disease 2019 (COVID-19) has seriously disturbed the daily life of the general population particularly the life of the pregnant women. Since the obstetricians and gynecologists (OBGYN) are often the primary health care providers during pregnancy, hence the importance of their critical role in preventing and managing COVID-19 infection in their patients.Objectives: This study aims to assess the knowledge, attitudes and practices of OBGYN in order to identify existing gaps and to improve patient and occupational safety.Methods: A cross-sectional study, using an online survey, was conducted during the rapid rise of COVID-19 pandemic in Lebanon over the period extending between 20th October and 20th November 2020 among Lebanese OBGYNs. The analysis was performed using Statistical Package for Social Sciences (SPSS) software. Data collected is subjected to descriptive analysis. A good level of knowledge was defined when the percentage of correct answers reaches more than 80% of the respondents.Results: A total of 279 OBGYNs participated in the survey of which 57% were males. The majority of them (64.2%) were aged more than 45 years, married (79.9%) with large work experience (70.3%). Only 28.3% were reluctant to take care of COVID-19 patient. Most of them feared of contracting COVID-19 or transmitting COVID-19 to their family member due to occupational exposure and 42.3% felt overwhelmed. 62.7% of them considered that policies implemented by the ministry of public health are sufficient. The majority of OBGYNs had a good level of knowledge in different basic and specific domains related to COVID-19 and pregnancy. Furthermore, a good practice score in all relevant aspects (personal, clinic and patient) was revealed.Conclusion: The high knowledge and practice scores, among Lebanese OBGYNs disclose a strong commitment from the part of these physicians to fulfill their responsibilities during this pandemic towards themselves and their patients.
Using Response Surface Methodology of Deep Hole Electrical Discharge Machining Study of Nickel-Base Alloy Inconel-718
Authors: Chen Shao Hsien , Huang Kuo-Tai 
Published: May 2021

Abstract
The blades in the high-pressure turbine section of engines of modern aerospace and defense industries need to be drilled for pressurization. The milling and drilling of nickel-base material Inconel-718 are likely to create tool wear and tear, so the drilling process of milling is replaced by deep hole electrical discharge machining (EDM) extensively. However, the EDM creates reaming or overcut phenomenon, so reducing overcut by parameter optimization is an important study. This study used will use response surface methodology to establish the influencing factors of machining parameters in the hole enlargement and machining speed. The experimental results show that the main influencing factor in the nickel-base alloy deep hole EDM is the discharge voltage (V), the secondary factor is the discharge current (I). The hole enlargement of processed hole without optimization is 60um~100um. The DOE is used for hole enlargement measurement and machining speed measurement to design important parameters, and to predict experiment data analysis. The half normal probability graph, Pareto chart and Analysis of variance (ANOVA) are used to learn about the significant factors of parameters and the influence of interaction. The outlet value of optimized parameters is relatively uniform, and the hole morphology is relatively free of residue stacking. Finally, the inlet and outlet results are improved by 17.9% compared with the original parameters. The optimization parameter value predicted by the fitted model is 0.0392mm, and the optimized upper and lower holes validation experiment machining error is 0.0380mm, and the values are quite close, proving that this prediction model is accurate. The model prediction of this DOE can enhance the applied technology of deep hole EDM for nickel-base alloy.
Evaluation of the Pathways for Survivors program to address breast cancer survivorship associated distress
Authors: Umashankar Saumya , Mamounas Matina Elise , Matthys Madeline , Hadeler Edward Kenji , Wong Emily Claire , Hicks Greg , Hwang Jimmy , Chien A. Jo , Rugo Hope S. , Hamolsky Deborah , Esserman Laura , Melisko Michelle 
Published: May 2021

Abstract
Purpose: Breast cancer patients not infrequently experience escalation of anxiety after completing curative treatment. This study evaluated the acceptability and psychological impact of a one-day workshop emphasizing behavioral strategies involving intention and self-efficacy.Methods: Breast cancer survivors attending a one-day Pathways for Survivors workshop, provided feedback, and completed electronic quality of life (QOL) questionnaires at baseline, 1- and 6-weeks, and 6-months post workshop. Attendees’ baseline QOL scores were compared to follow up (FUP) scores. Scores from patients receiving routine FUP care were also compiled as a reference population.Results: Seventy-seven patients attended one of nine workshops. Mean satisfaction was 9.7/10 with the workshop, and 9.96/10 with the moderator. Participants’ baseline mean Patient-Reported Outcomes Measurement Information System (PROMIS) anxiety and depression scores were 57.8 ± 6.9 and 55.3 ± 7.5, significantly higher than routine FUP care patients (49.1 ± 8.3 and 47.3 ± 8 respectively). PROMIS anxiety and depression scores decreased and Happiness Index Profile (HIP-10), measuring intention and resiliency, increased significantly at 1- and 6-week FUPs.Conclusions: Pathways for Survivors was favorably received. Anxiety and depression decreased significantly at 1- and 6-weeks post workshop and remained below baseline at 6 months. Increased HIP-10 scores support that patients incorporated skills from the workshop. A one-day workshop led by a lay moderator significantly improved several psychological measures, suggesting it may be a useful and time-efficient strategy to improve QOL in breast cancer survivors. We are investigating whether an abbreviated “booster” of the intervention at a later date could further improve and maintain QOL gains.
Clinical Impact of Rare Variants Associated with Inherited Channelopathies: A Five-Year Update
Authors: sarquella georgia , Fernandez Anna , cesar sergi , arbelo elena , coll monica , Perez Alexandra , Puigmule Marta , Iglesias Anna , Alcalde Mireia , Vallverdu Marta , Fiol Victoria , Ferrer Carles , olmo Bernat del , Pico Ferran , Lopez Laura , garcia ana , jorda paloma , tiron coloma , toro rocio , grassi simone , oliva antonio , brugada josep , brugada ramon , campuzano oscar 
Published: May 2021

Abstract
A proper interpretation of the pathogenicity of rare variants is crucial before clinical translation. Ongoing addition of new data may modify previous variant classifications; however, how often a reanalysis is necessary remains undefined. We aimed to extensively reanalyze rare variants associated with inherited channelopathies originally classified five years ago and its clinical impact. In 2016, rare variants identified through genetic analysis were classified following the American College of Medical Genetics and Genomics’ recommendations. Five years later, we have reclassified the same variants following the same recommendations but including new available data. Potential clinical implications were discussed. Our cohort included fourty-nine cases of inherited channelopathies diagnosed in 2016. Update show that 18.36% of the variants changed classification mainly due to improved global frequency data. Reclassifications mostly occurred in minority genes associated with channelopathies. Similar percentage of variants remain as deleterious nowadays, located in main known genes (SCN5A, KCNH2 and KCNQ1). In 2016, 69.38% of variants were classified as unknown significance, but now, 53.06% of variants are classified as such, remaining the most common group. No management was modified after translation of genetic data into clinics. After five years, nearly 20% of rare variants associated with inherited channelopathies were reclassified. This supports performing periodic reanalyses of no more than five years since last classification. Use of newly available data is necessary, especially concerning global frequencies and family segregation. Personalized clinical translation of rare variants can be crucial to management if a significant change in classification is identified.
Efficacy of The Acupressure Wrist-Ankle Strap Combined With CBTI In Mild Insomnia Patients With Anxiety Disorders: Study Protocol For A Randomized Controlled Trial
Authors: Yuan Ying , Zhou Qinghui , Fang Fanfu , Li Weihong , You Yanli 
Published: May 2021

Abstract
Background: Insomnia is very common in current society, and patients are often accompanied by a certain degree of anxiety, depression, etc. Recent studies have found that the hypothalamic-pituitary-adrenal (HPA) axis excitement-inhibition state is an important indicator of sleep quality. Wrist-ankle acupuncture (WAA) is safe and effective for insomnia. The acupressure wrist-ankle straps, based on WAA theory, is a portable WAA point compression strap, which can treat diseases by automatically applying pressure to the treatment location and be operated by patients themselves. We design this trial to evaluate the clinical effect of the acupressure wrist-ankle strap in the treatment of mild insomnia patients with anxiety disorders. Methods/design: This trial is a parallel-design, patients-assessor blinded, randomized, sham-controlled. In total, 108 patients diagnosed with mild insomnia patients with anxiety disorders will be randomly equally assigned into two groups, acupressure wrist-ankle strap group or non-acupressure wrist-ankle strap group; they will receive treatments for 8 weeks with 5 sessions each week. Rating scales, sleep monitor and laboratory tests will be used to observe the clinical effect. From the perspective of the circadian secretion of peripheral blood-related hormones in the hypothalamic- pituitary- adrenal (HPA) axis, the possible mechanism of acupressure wrist-ankle straps for treating insomnia is studied.Discussion: The results of this study will confirm the efficacy of acupressure wrist-ankle strap combined with CBTI in the treatment of mild insomnia patients with anxiety disorder and whether its mechanism is related to HPA axis. The acupressure wrist-ankle strap may become a pure physical, no side effect treatment of mild insomnia.Trial registration: Chinese Clinical Trial Registry (identifier: ChiCTR2000039352; registration date: 24 October 2020).
Hub-and-spoke social networks among Indonesian cocoa farmers homogenize farming practices
Authors: Matous Petr , Bodin Örjan 
Published: May 2021

Abstract
Smallholder farms support the livelihoods of 2.5 billion people and their decisions on fertilizers use have profound sustainability implications. We investigated if and how social influence exerted through peer-to-peer information exchange affect the use of fertilizer among 2734 Indonesian cocoa farmers across 30 different villages. Results show that the structures of these village-based social networks strongly relate to farmers’ use of fertilizer. In villages with highly centralized networks (i.e. where one or very few farmers holds disproportionately central position in the village network), a large majority of farmers report the same fertilizer use. By contrast, in less centralized networks, fertilizer use varies widely. The observed community-level distributions of fertilizer use are consistent with complex contagion mechanisms in which social influence is only exerted by opinion leaders that are much more socially connected than others. Our findings suggest significant policy implications for development programs targeting smallholder farming communities.
Direct Anterior Approach for Total Hip Arthroplasty in the Lateral Decubitus Position Versus Supine Position: A Random Clinical Trial
Authors: Xiao Yao , Li Zhanglai , Lin Feitai , Zhang Yiyuan , Weng Yan , Chen Jinhua , Feng Eryou 
Published: May 2021

Abstract
Background: Direct anterior approach (DAA）for total hip arthroplasty (THA）could be performed either in the lateral decubitus position or supine position. However, there is an obvious absence of literature regarding the differences, which position may be more conducive to recovery, technically more demanding, associated with component malposition or more complications. Methods: From Jan.1st, 2020 to oct.1st, 2020, 45 patients were recruited for primary unilateral THA using the DAA. In total, 27 patients (60%) underwent THAs using the DAA in the supine position, and 18 patients (40%) in the lateral decubitus position. All surgeries were performed by a high-volume surgeon. Technical information, clinical and radiographic outcomes, SF-12 and patient-reported outcomes such as WOMAC were evaluated. All the date were tested with Generalized Linear Mixed Models Analysis, GLM Repeated Measurement Analysis, Independent samples t-test or Pearson’s chi-square test.Results: There were no differences in Population characteristics before surgery. The operation time, length of stay and blood loss in the LP group and the SP group were no differences. The prosthesis of the two groups were in a good position. pre-operative and the first and third day after the surgery of CK-MB and Hb,pre-operative and the last follow-up of HSS,WOMAC,UCLA,VAS,SF-12,and pre-operation and post-operation of Offset,FA and LLD, all the above indicators show no significant difference. And the incidence of complications in the lateral position was lower than that in the supine position.Conclusion: Both THA via DAA in the lateral decubitus position and in the supine position produced excellent clinical outcomes. From the perspective of the occurrence of complications, we are more inclined to use the lateral position.
Therapeutic Potential of an Intestinotrophic Hormone, Glucagon-Like Peptide 2, for Treatment of Type 2 Short Bowel Syndrome Rats with Intestinal Bacterial and Fungal Dysbiosis
Authors: Hu Xiuting , Cheng Wei , Fan Shengxian , Huang Yuhua , Chen Xi , Jiang Zhiwei , Wang Jian 
Published: May 2021

Abstract
Background: Previous studies showed that type 2 short bowel syndrome (SBS) rats were accompanied by severe intestinal bacterial dysbiosis. Limited data are available for intestinal fungal dysbiosis. Moreover, no effective therapeutic drugs are available for these microbiota dysbiosis. The aims of our study were to investigate the therapeutic potential of glucagon-like peptide 2 (GLP-2) for these microbiota dysbiosis in type 2 SBS rats.Methods: 8-week-old male SD rats which underwent 80% small bowel resection, ileocecum resection, partial colon resection and jejunocolostomy, were treated with saline (SBS group, n=5) or GLP-2 (GLP2.SBS group, n=5). The Sham group rats which underwent transection and re-anastomosis were given a saline placebo (Sham group, n=5). 16S rRNA and ITS sequencing were applied to evaluate the colonic bacterial and fungal composition at 22 days after surgery, respectively.Results: The relative abundance of Actinobacteria, Firmicutes and proinflammatory Proteobacteria increased significantly in SBS group rats, while the relative abundance of Bacteroidetes, Verrucomicrobia and Tenericutes decreased remarkably. GLP-2 treatment significantly decreased Proteus and increased Clostridium relative to the saline treated SBS rats. The diversity of intestinal fungi was significantly increased in SBS rats, accompanied with some fungi abnormally increased and some resident fungi (e.g., Penicillium) significantly decreased. GLP-2 treatment significantly decreased Debaryomyces and Meyerozyma, and increased Penicillium. Moreover, GLP-2 partially restored the bacteria-fungi interkingdom interaction network of SBS rats.Conclusion: Our study confirms the bacterial and fungal dysbiosis in type 2 SBS rats, and GLP-2 partially ameliorated these microbiota dysbiosis.
Lysosomal-Immune Axis Is Associated with COVID 19 Disease Severity: Insights from Patient Single Cell Data
Authors: Pande Rahul , Teeple Erin , Huang Weixiao , Klinger Katherine , Rajpal Deepak , Kumar Dinesh 
Published: May 2021

Abstract
SARS-COV-2 has become a leading cause of illness, hospitalizations, and deaths worldwide yet heterogeneity in disease morbidity remains a conundrum. In this study, we analyzed publicly available single-cell RNA-seq data from 75076 cells sequenced from clinically staged COVID-19 patients using a network approach and identified lysosomal-immune axis as a factor significantly associated with disease severity. Our results suggest modulation of lysosomal-immune pathways may present a novel drug-targeting strategy to attenuate SARS-Cov-2 infections.
Plasma Levels of D-dimer and Fibrin Degradation Products Correlate with Bullous Pemphigoid Severity: a Cross-sectional Study
Authors: Wang Sijia , Lu Mei , Zhao Zijun , Peng Xueting , Li Liang , Fang Min , Xia Yumin , Liu Yale , Cheng Chuantao 
Published: May 2021

Abstract
Background Bullous pemphigoid (BP), the most frequent blistering dermatosis in the elderly, is associated with increased mortality. The severity of BP can be assessed by detecting the anti-BP180 immunoglobulin G (IgG) titer, but the lab test is not available in many community clinics. BP patients are usually in a hypercoagulable state with increased levels of D-dimer and fibrin degradation products (FDPs). ObjectivesTo evaluate the use of D-dimer and FDPs in assessing BP severity. Methods We compared the levels of plasma D-dimer, FDPs, eosinophils, and anti-BP180 IgG titer between 48 BP patients and 33 Herpes zoster (HZ) patients (control group). Correlational analyses were conducted to determine the relationships between the lab values and BP.ResultsThe plasma D-dimer and FDP levels were higher in BP patients than in HZ controls (D-dimer: 3297 ±2517 µg/L vs. 569.70 ±412.40 µg/L; FDP: 9.74 ±5.88 mg/L vs. 2.02 ±1.69 mg/L, respectively, P<0.0001). Significant positive correlations were found between D-dimer/FDP levels and BP severity markers (anti-BP180 IgG titer [D-dimer: r=0.3928, P=0.0058; FDP: r=0.4379, P=0.0019] and eosinophil counts [D-dimer: r=0.3625, P=0.0013; FDP: r=0.2880, P=0.0472]) in BP patients. We also found an association between FDP and urticaria/erythema lesions (r=0.3016, P=0.0372), but no other BPDAI components. In 19 BP patients with complete remission after systemic glucocorticoid treatment, D-dimer and FDP levels decreased post-therapy (D-dimer: 5559 ±7492µg/L vs. 1738 ±1478 µg/L; P<0.0001; FDP: 11.20 ±5.88 mg/L vs. 5.13 ±3.44 mg/L; P=0.0003), where as they did not in BP patients with treatment resistant.Conclusion Plasma D-dimer and FDP are convenient markers to evaluate BP severity.