bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome and Metabolomic Analysis to Reveal the Browning Spot Formation of ‘Huangguan’ Pear
Authors: Wang Qi , Wu XinYi , Liu Li , Yao Daozhi , Li Jinchao , Fang Jie , Chen Xiaonan , Zhu Liwu , Liu Pu , Ye Zhenfeng , Jia Bing , Heng Wei 
Published: January 2021

Abstract
Background: Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused on physiological and biochemical aspects, and the molecular mechanism is unclear.Result: In the present study, the structural characteristics of ‘Huangguan’ pear with BS were observed with SEM, the water loss and brown spot were evaluated, and transcriptome and metabonomics analyses were conducted to reveal the molecular mechanism of ‘Huangguan’ pear skin browning disorder. The results showed that the occurrence of BS was accompanied by a decrease in the wax layer and an increase in lignified cells. It appears that genes related to wax biosynthesis were down-regulated in BS, resulting in the decrease of wax layer in BS. Genes related to lignin were up-regulated at transcriptional level, resulting in upregulation of metabolites related to phenylpropanoid biosynthesis. Expression of calcium-related genes were upregulated in BS. The cold-induced genes CS120, LTI65 and RCI2B may be the key genes that induce BS. In addition, the results demonstrated that exogenous NaH2PO4·2H2O and ABA treatment could inhibit the incidence of BS during harvest and storage time by increasing the expression of wax-related genes and calcium-related genes and increasing plant resistance, whereas GA3may accelerate the incidence and index of BS in transcriptomics.Conclusions: The results of this study would provide a basis for the molecular mechanism of BS formation and clarify the effects of different treatments on the incidence and molecular regulation of BS.
Long Stent Graft for Frozen Elephant Trunk Repair in Acute Type A Aortic Dissection
Authors: Wang Chaojie , Zhang Wenqian , Peng Jihai , He Jie , Chen Guangtian , Huang Jinsong , Wu Min , Fan Ruixin , Fan Xiaoping 
Published: January 2021

Abstract
Background: The frozen elephant trunk (FET) technique has become an important tool in the treatment of acute type A aortic dissection. The aim of this study was to evaluate the effect of long FET on spinal cord injury (SCI) and distal aortic remodeling after acute type A aortic dissection based on clinical and radiological outcomes.METHODS: From January 2018 to November 2019, 158 patients [mean age 51.8 years (range 32 - 78 years), 88.6% male] with acute type A aortic dissection were treated by FET with 100 mm (n=113) or 150 mm (n=45) open hybrid stent graft prosthesis. Patients were divided into two groups according to the length of FET. The clinical and radiological outcomes of the patients were reviewed retrospectively. RESULTS: Postoperative outcomes did not differ significantly: in-hospital mortality (9.7% vs 6.7%, P=0.758) and SCI (5.3% vs 2.2%, P=0.674). Aortic remodeling, which was evaluated by aortic diameter, true lumen diameter, false lumen diameter and the rate of false lumen complete thrombosis, was more positive in long FET group in the descending thoracic aorta during the follow-up period. At the abdominal level, there was no statistically significant difference between the two groups. In the long FET group, the level of complete false lumen thrombosis was more extensive during the follow-up period. CONCLUSIONS: The long version of FET does not increase the risk of SCI in patients with acute type A aortic dissection. The application of long FET can achieve better results in terms of remodeling of the thoracic aorta in the short- and medium-term follow-up.
Epidemiological Trends of Urinary Tract Infections at the Global, Regional, and National Levels from 1990-2017: A Population-Based Study
Authors: Zhu Cong , Huang Qiao , Luo Li-Sha , Deng Tong , Gu Jia-Min , Tang Shi-Di , Li Lu-Yao , Zi Hao , Zeng Xian-Tao 
Published: January 2021

Abstract
BackgroundUrinary tract infections (UTIs) are some of the most common infections worldwide and consume a lot of medical resources every year. However, there were a lack of available data on its incidence and disease burden. We armed to investigate incidence, mortality, and disability adjusted life-years (DALYs) of urinary tract infections (UTIs) from 1990 to 2017.MethodsWe extracted data from the Global Burden of Disease Study 2017, then calculated estimated annual percentage changes (EAPC) of age-standardized incidence rate (ASIR), age-standardized death rate (ASDR), and age-standardized DALYs rate at global, national, regional, and socio-demographic index (SDI) level.ResultsFrom 1990 to 2017, the globally incident cases (+52.09%), death cases (+140.10%), and DALYs (+69.65%) of UTIs all increased. The ASIR, ASDR, and age-standardized DALYs rate showed upward trends with the EAPC of +0.10 (95%CI: 0.07 to 0.12), +0.72 (95%CI: 0.65-0.78), and +0.06 (95%CI: -0.05 to 0.16), respectively. The ASIR decreased only in the high-middle SDI quantile (-0.26, 95%CI: -0.3 to -0.23). United Arab Emirates had the largest increase of DALYs (+835.04%), but Bulgaria had the largest decrease (-80.74%). EAPC for incidence and mortality were below 0 mainly in Europe and East Asia. In 2017, the incident cases (+3.44 times), the deaths (+1.31 times), and DALYs (+1.21 times) were all higher in females than males. The incident cases were mainly concentrated in 15-49 years old; DALYs and mortality were higher in over 80 age groups.Conclusions﻿Globally, the burden of UTIs increased from 1990 to 2017, especially in females; however, distinct varies were observed in different regions and countries. The infants and elders are easier to die when they suffer from UTIs.
Does emotion before a chemotherapy course break a heart? Cardiogenic shock complicating Takotsubo cardiomyopathy
Authors: Taamallah Karima , Ghedira Hela , Arous Younes , Haggui Abdeddayem , Chenik Sarra , Hajlaoui Nadhem , Fehri Wafa 
Published: January 2021

Abstract
Introduction: Takotsubo cardiomyopathy is characterized by a broad spectrum of clinical presentations. Despite its reversibility, it is associated with severe adverse in-hospital events. Recent studies have identified a high incidence of TTC in cancer patients.Case report: We report the case of a 59-year-old-man with TTC occurring just before injecting of chemotherapy drug complicated by cardiogenic shock and acute heart failure. Under medical treatment, a complete recovery of cardiac contractility was noted within three days. chemotherapy regimen has been stopped and then alleviated from RCHOP to the RCVD. No recurrence of cardiac symptoms was noted during the follow-up period. Left ventricle ejection fraction, BNP, and troponin levels were maintained normal.Conclusion: Takotsubo cardiomyopathy can occur in cancer patients leading to interruption of chemotherapy because of left ventricular dysfunction, which may adversely affect the oncologic outcome because of interruption of cancer treatment. We report this case to remind clinicians that TTC should be considered in the differential diagnosis of chest pain in cancer patients who present with ECG findings characteristic of the acute coronary syndrome. We hope to facilitate prompt diagnosis for an early intervention that allows rapid recovery of myocardial function, and resume cancer therapy early and may help lower mortality.
The Role of Making Voluntary Function in Prognosticating and Preventing from Addiction Potential: A Survey Study Among Some Members of the Iranian Red Crescent Society
Authors: MamSharifi Peyman , Sohrabi Faramarz , Seidi Pegah. , Borjali Ahmad , Hoseininezhad Nahid , Rezaeifar Hanieh , Asadi Nazanin 
Published: January 2021

Abstract
Background: Considering Voluntary Function (VF), the purpose of the present study was to prognosticate the addiction potential (AP) among some members of the Iranian Red Crescent Society (IRCS) in Iran. To get the research done, we made use of both correlative and descriptive methods as research methods. Methods: The statistical population of the current study included all youths who have been members of the IRCS. We employed multistage cluster sampling as the sampling method. We recruited 620 active volunteers of the IRCS from 31 province and 175 cities of Iran (48.7% female and 50.1 Male mean age 23.27±3.32, range 14–31 years). Iranian Addiction Potential Scale (IAPS) and Voluntary Function Inventory (VFI) have assisted us in collecting research data. Results: Findings proved that there was a negative significant correlation between the AP and all measurements of VF such as protective enhancement, understanding, career, values, and motives; meaning that the more time youth spent on participating in voluntary activities, the less likely they sought to resort to misusing AP. Findings of the simultaneous-entry multiple regression has proved that volunteerism could prognosticate 15% of changes in the AP as a criterion variable. Conclusions: Due to its positive features, volunteerism played a key role in prognosticating and preventing the AP. Thus, it is incumbent to draw particular attention to this worthy factor.
BioData Mining | Short Report (Biomedcentral.Com) Combinatorial Analyses Identify A Core Response to the CDX2 Homeoprotein During Development and In Pathologies
Authors: GOURAIN Victor , DULUC Isabelle , DOMON-DELL Claire , FREUND Jean-Noel 
Published: January 2021

Abstract
Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in the evolutionary, developmental and therapeutic perspective. Here, we addressed this question by focusing on the CDX2 homeobox gene that encodes a transcription factor with important functions during embryonic development and in adult pathologies. By analysing RNAseq data and ChIPseq data, we unveiled the existence of a core of genes whose expression is responsive to the Cdx2 homeoprotein and also a core of chromosomal regions targeted by Cdx2 at three important steps of mouse development: trophectoderm formation, posterior body elongation and intestinal specification. In addition, based on human cancer data, we highlighted the relevance of the core of responsive genes by displaying a significant number of human orthologues exhibiting an altered expression along with CDX2 in human malignancies.
Antimicrobial susceptibility profiles, presence of integrons and associated cassette genes among Acinetobacter baumannii isolates from Southern part of Iran, Shiraz
Authors: Khoramrooz SeyedSajjad , Eslami Saba , Motamedifar Mohammad , Bazargani Abdollah , Zomorodian Kamiar 
Published: January 2021

Abstract
Background

Acinetobacter baumannii is a global concern to cause the health-care-associated infections, due to multidrug resistance against available commercially antimicrobial agents. Regarding this, the present study was conducted to determine the antimicrobial susceptibility of A.baumannii isolates from clinical specimens in Shiraz, and explore the possible relationship of susceptibility patterns with the presence of integrons and related gene cassettes.
Methods

A.baumannii isolates were collected, and their susceptibility to various antibiotics was tested using Kirby-Bauer disk diffusion method. Also, molecular analyses were performed to detect the presence of OXA-51 like gene, class I, II and III integrons, and associated gene cassettes.
Results

Majority of isolates demonstrated resistance to imipenem(99.4%),piperacilin(98.2%),gentamycin (98.2%) meropenem (97.7%)ceftazidime(95.4%)amikacin(95.4%) and trimethoprim-sulfamethoxazole (90.8%). All strains showed multidrug-resistance to most of the tested antibiotics. The distribution analysis of integrons genes showed that 90.2%, 72.4% and 12.1% of isolates carried the intI 1, intI2 and intI3 genes, respectively. Moreover, two types of prevalent gene cassettes including aad and dfr were detected in Class 1 integron-carrying strains.
Conclusions

The current study showed the high prevalence of A.baumannii isolates harboring integrons in our investigated medical center, which may propel distribution of multidrug resistance event. The different types of gene cassette arrays in the present study spotlight the remarkable role of geographical issues in MDR isolates dissemination. This subject could attribute to choose appropriate therapeutic interventions in different areas. Obtained data highlighted the necessity for continuous surveillance to prevent distribution of multidrug resistance among A.baumannii strains in Iran.
Sustainment of Proactive Physical Therapy for Individuals With Early-Stage Parkinson’s Disease: A Quality Improvement Study Over 4 Years
Authors: MacDonald Jillian , Doyle Laura , Moore Jennifer L , Rafferty Miriam R 
Published: January 2021

Abstract
Background: Implementation science frameworks aided the development of a new, evidence-based clinical physical therapy program. The purpose of this report is to describe the process of sustaining a clinical program in practice for over four years. We present a framework for integrating tools for sustainability with the Knowledge-to-Action model in the context of a proactive physical therapy program (PAPT) for individuals with early stage Parkinson’s disease.Methods: Sustainability of implementation strategies was addressed using the Dynamic Sustainability Framework and sustainability assessment tools. Repeated retrospective medical record reviews and semi-structured interviews were used to evaluate the reach and adoption of the PAPT over four years. Characteristics of those who engaged with PAPT, implementation fidelity, and clinical effectiveness were assessed for Year 1 and Year 3. Sustainability was measured using RE-AIM, NHS Sustainability Model, and Clinical Sustainability Assessment Tool.Results: Reach increased from 28 to 110 total patients per year and spread occurred from one to three sites. PAPT user age, sex, Hoehn & Yahr rating, time since diagnosis and type of insurance were similar in Year 1 and Year 3 (p>0.05). The program sustained its effect to help participants increase or maintain self-reported exercise (Y1: 95%, Y3: 100%). However, upon evaluation PAPT users in Year 3 had longer time since symptom onset and worse UPDRS motor scores compared to the PAPT users in Year 1 (p<0.05). All sites sustained the core intervention components, with sustainability scores of 71/100 (± 9.9) on the NHS Sustainability Model and 6.1/7 (± 0.9) on the Clinical Sustainability Assessment Tool.Conclusions: Integrating multiple sustainability models and assessments supported continued effectiveness, spread, and sustainment of PAPT for four years. Effective planning, anticipating common healthcare changes, and addressing sustainability determinants early in program implementation were essential aspects of long-term success.
Genetic Characterization of Multidrug-Resistant Salmonella Typhimurium Carrying Mcr-1 in China
Authors: Haoran Zhang , Yong Huang , Beibei Liang , Jing Xie , Ying Xiang , Xinying Du , Chaojie Yang , Hongbo Liu , HONGBo Liu , Hui Wang , Mingjuan Yang , Ligui Wang , Xiaofeng Hu , Lianqun Jin , Zhengquan Yuan , Shaofu Qiu , Hongbin Song 
Published: January 2021

Abstract
Background: With the rapid emergence of plasmid mediated polymyxin resistance gene mcr-1, multidrug-resistant Salmonella caused great troubles in clinical treatment and attracted extensive attention. Here we report multidrug-resistant Salmonella strains harboring mcr-1 in China, which are resistant to both polymyxin, traditional antibiotics and even clinically wide-used antibiotics. Methods: We screened 1454 strains of Salmonella collected in our laboratory from 2006 to 2018 from 3 provinces or regions for mcr-1 by PCR. Antimicrobial susceptibility testing was determined. Plasmid conjugation assays were carried out to analysis the transferability of polymyxin resistance. Genetic polymorphism analysis of Salmonella was performed using the PFGE, and the plasmid profiles were characterized by S1-PFGE and southern blotting. The plasmids harboring mcr-1 were sequenced and compared. Results: Eleven S. Typhimurium isolates harboring mcr-1 with polymyxin resistance (MICs 4μg/ml) were identified from intestinal infections and foods in China. All S. Typhimurium isolates were multidrug-resistant to traditional antibiotics and even clinically wide-used antibiotics. Three types of plasmids harboring mcr-1 were recovered (IncHI2, IncX4 and IncI2). Compared with the reference plasmid, IncX4 and IncI2 plasmids had extremely similar typical backbone, and contain only mcr-1 resistance gene. However, IncHI2 were the most diverse type of plasmid due to containing a large MDR region, including blaCTX-M, oqxB, sul, aph, aadA and blaTEM. IncHI2 plasmids were observed to contain only one or no insertion sequence ISApl1 around mcr-1, without forming a circular intermediate. Conclusion: With the horizontal transfer of different types of plasmids, mcr-1 is widely spread worldwide. These prevalent plasmids are responsible for resistance to polymyxin, traditional antibiotics and even clinically wide-used antibiotics resulting from transmission of mcr-1 and other resistance genes. Our studying emphasizes the necessity to jointly monitor its international epidemic and preemptive further upgrade.
Hypothalamus-pituitary Dysfunction as an Independent Risk Factor for Postoperative Central Nervous System Infections in Patients with Sellar Region Tumors
Authors: Wen Junxian , Yin Rui , Chen Yihao , Chang Jianbo , Ma Baitao , Zuo Wei , Zhang Xiao , Ma Xiaojun , Feng Ming , Wang Renzhi , Ma Wenbin , Wei Junji 
Published: January 2021

Abstract
Objective: The purpose of this study was to verify that hypothalamus-pituitary dysfunction is one of the risk factors for postoperative central nervous system infections (PCNSIs).Method: We performed a retrospective analysis of all patients with sellar region lesions who underwent surgery between January 2016 and November 2019 at Peking Union Medical College Hospital. In total, 44 age‑ and sex-matched controls were enrolled. Univariate and multivariate analyses were performed to identify risk factors for PCNSIs.Result: We enrolled 88 patients, 44 of whom had PCNSIs. Surgical approach (TCS) (P<0.001), previous surgery on the same site (P=0.001), intraoperative cerebral spinal fluid (CSF) leakage (P<0.001), postoperative adrenal insufficiency (P=0.017), and postoperative DI (P=0.004) correlated significantly with PCNSIs. Multivariate analysis showed that intraoperative CSF leakage (OR:13.754; 95%CI: 3.482-54.328; P<0.001), postoperative diabetes insipidus (DI) (OR: 6.261; 95%CI: 1.114-35.189; P=0.037) and postoperative adrenal insufficiency (OR: 7.153; 95%CI: 1.071-47.764; P=0.042) were independent influencing factors for PCNSIs.Conclusion: Intraoperative CSF leakage, postoperative DI and postoperative adrenal insufficiency are risk factors for PCNSIs in patients with sellar region tumors.
Efficacy and Safety of Tocilizumab in Patients with COVID-19: A Systematic Review and Meta-Analysis.
Authors: Li Zhenlu , Che Qianqiu , Li Mao , Liu Jianping , Du Rao , Yue Chao , Zhang Ling , Li Hongwei , Zhao Liming , Hu Weiming , Lu huimin , Xiong Junjie 
Published: January 2021

Abstract
Background Tocilizumab (TCZ) is an anti-interleukin-6 antibody that has been used to treat patients with 2019 coronavirus disease (COVID-19). Numerous retrospective studies have shown beneficial treatment efficacy. Several recent randomized clinical trials have questioned the efficacy of TCZ in patients with COVID-19. Therefore, we performed an updated systematic review and meta-analysis to explore the effectiveness and safety of tocilizumab recently used for treating patients with COVID-19. Methods Randomized clinical trials (RCTs) and comparative studies that compared the outcomes between TCZ and standard of care (SOC) were analysed. PubMed, EMBASE, and the Cochrane Library (inception to November 20, 2020) were systematically searched. Primary outcomes included mortality and the rate of requirement for mechanical ventilation (MV). In addition, several subgroup analyses stratified by disease severity, publication type and TCZ administration were performed. Results Three RCTs, twenty-one cohort studies and nine case-control studies including 11,206 patients were finally included. The TCZ group included 2,794 patients (24.93%) and the SOC group included 8,412 patients (75.07%). The mortality rate (>14 days) of the TCZ group, 29.63% (590/1,991), was lower than the SOC group, 41.51% (2,380/5,734) (OR 0.64, 0.57 to 0.73; p <0.00001). However, no significant difference in-14-day mortality rates was observed between the two groups (13.53% vs 22.92%, p = 0.21). Meanwhile, the rate of MV was significantly decreased in the TCZ group compared with the SOC group (OR 0.42, 0.22 to 0.83; p = 0.01). According to the results of the subgroup analysis stratified by disease severity, TCZ only reduced the mortality rate for critical patients with COVID-19 compared with SOC (OR 0.60, 0.52 to 0.71; P < 0.00001), particularly for patients in the intensive care unit (ICU) or patients requiring MV. No statistically significant increase was recognized in the rates of secondary infections or thrombosis between the two groups. Conclusions This systematic review and meta-analysis found that the addition of tocilizumab to the SOC might reduce mortality after 14 days in patients with COVID-19, particularly critical patients requiring MV. More extensive RCTs with longer follow-up periods are needed to validate these findings.
The prevalence and risk factors for anxiety in frontline nurses under COVID-19 pandemic based on a large cross-sectional study using the propensity score-matched method
Authors: Zhao Qingyun , Yao Zichuan , Zhu Xianqing , Jiang Yunzhong , Pan Chunyu 
Published: January 2021

Abstract
Introduction: We determined the prevalence of anxiety and the associated risk factors in in frontline nurses under COVID-19 pandemic. Methods: This cross-sectional study was conducted from February 20, 2020 to March 20, 2020 and involved 562 frontline nurses. The effective response rate was 87.68%. After propensity score matched, there were 532 participants left. Extensive characteristics, including demographics, dietary habits, life-related factors, work-related factors, and psychological factors were collected based on a self-reported questionnaire. Specific scales measured the levels of sleep quality, physical activity, anxiety, perceived organization support and psychological capital. Adjusted odds ratios and 95% confidence intervals were determined by binary paired logistic regression.Results: Of the nurses enrolled in the study, 33.60% had anxiety. Five independent risk factors were identified for anxiety: poor sleep quality (OR=1.235), experienced major events (OR=1.653), lower resilience and optimism of psychological capital (OR=0.906, and OR=0.909) and no visiting friend constantly (OR=0.629). Conclusions: This study revealed a considerable high prevalence of anxiety in frontline nurses during the COVID-19 outbreak, and identified five risk factors, which were poor sleep quality, experienced major events, lower resilience and optimism of psychological capital, and no visiting friend rarely. Protecting mental health of nurses is important for COVID-19 pandemic control and their wellbeing. These findings enrich the existing theoretical model of anxiety and demonstrated a critical need for additional strategies that could address the mental health in frontline nurses for policymakers.