bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALC1 links chromatin accessibility to PARP inhibitor response in homologous recombination deficient cells
Authors: Verma Priyanka , Zhou Yeqiao , Cao Zhendong , Deraska Peter V. , Deb Moniher , Arai Eri , Li Weihua , Shao Yue , Li Yiwen , Puentes Laura , Patankar Sonali , Mach Robert H. , Faryabi Robert B. , Shi Junwei , Greenberg Roger A. 
Published: July 2021

AbstractThe response to Poly (ADP-ribose) polymerase inhibitors (PARPi) is dictated by homologous recombination (HR) DNA repair mechanisms and the abundance of lesions that trap PARP enzymes on chromatin. It remains unclear, however, if the established role of PARP in promoting chromatin accessibility impacts viability in these settings. Using a CRISPR based screen, we identify the PAR-binding Snf2-like ATPase, ALC1/CHD1L, as a key determinant of PARPi toxicity in HR-deficient cells. ALC1 loss reduced viability of BRCA mutant cells and enhanced their sensitivity to PARPi by up to 250-fold, while overcoming several known resistance mechanisms. ALC1 loss was not epistatic to other repair pathways that execute the PARPi response. Instead, ALC1 deficiency reduced chromatin accessibility concomitant with a decrease in the association of repair factors. This resulted in an accumulation of replication associated DNA damage and a reliance on HR. These findings establish PAR-dependent chromatin remodeling as a mechanistically distinct aspect of PARPi responses, implicating ALC1 inhibition as a new approach to overcome therapeutic resistance in HR-deficient cancers.
Use of autologous platelet derivatives for secondary alveoloplasty in patients with cleft lip and palate: a protocol for a systematic review and meta-analysis
Authors: Campolo Andrés , Heider Claudia , Cañete Ismael , Verdugo María Francisca , Jeria Rocio Bravo , Morovic Carmen Gloria , Rada Gabriel 
Published: July 2021

AbstractObjectiveTo assess the effectiveness and safety of autologous platelet derivatives, specifically platelet-rich plasma (PRP) or platelet-rich fibrin (PRF), for secondary alveoloplasty in patients with cleft lip and palate.Eligibility criteriaWe will include randomized trials evaluating the effect of autologous platelet derivatives on newly bone formed after secondary alveoloplasty in cleft lip and palate patients. Two reviewers will independently screen each study for eligibility, data extraction, and bias assessment using the Cochrane “risk of bias “tool. We will pool the results using meta-analysis and apply the GRADE system to assess the certainty of the evidence for each outcome.Data sourcesA comprehensive search will include all relevant randomized controlled trials (RCTs), the ongoing investigation reported in specialty congresses and trials regardless of language or publication status (published, unpublished, in press, and progress). We will conduct searches in the following databases: Cochrane Central Register of Controlled Trials (CENTRAL), PUBMED, Embase, and LILACS. We will screen trial registries and other sources in order to identify articles that might have been missed in the electronic searches.Ethics and disseminationAs researchers will not access information that could identify an individual participant, obtaining ethical approval was waived.
Recovery of serum testosterone levels is an accurate predictor of survival from COVID-19 in male patients
Authors: Toscano-Guerra Emily , Gallo Mónica Martínez , Arrese-Muñoz Iria , Giné Anna , Díaz Noelia , Gabriel-Medina Pablo , Riveiro-Barciela Mar , Labrador-Horrillo Moisés , Martinez-Valle Fernando , Hernández-González Manuel , Borrell Ricardo Pujol , Rodríguez-Frias Francisco , Ferrer Roser , Thomson Timothy M. , Paciucci Rosanna 
Published: July 2021

AbstractInfection with SARS-CoV-2 portends a broad range of outcomes, from a majority of asymptomatic cases or mild clinical courses to a lethal disease. Robust correlates of severe COVID-19 include old age, male sex, poverty and co-morbidities such as obesity, diabetes or cardiovascular disease. A precise knowledge is still lacking of the molecular and biological mechanisms that may explain the association of severe disease with male sex. Here, we show that testosterone trajectories are highly accurate individual predictors (AUC of ROC = 0.928, p < 0.0001) of survival in male COVID-19 patients. Longitudinal determinations of blood levels of luteinizing hormone (LH) and androstenedione suggest an early modest inhibition of the central LH-androgen biosynthesis axis in a majority of patients, followed by either full recovery in survivors or a peripheral failure in lethal cases. Moreover, failure to reinstate physiological testosterone levels was associated with evidence of impaired T helper differentiation and decrease of non-classical monocytes. The strong association of recovery or failure to reinstate testosterone levels with survival or death from COVID-19 in male patients is suggestive of a significant role of testosterone status in the immune responses to COVID-19.
Establishment of Strigolactone-Producing Bacterium-Yeast Consortium
Authors: Wu Sheng , Ma Xiaoqiang , Zhou Anqi , Valenzuela Alex , Zhou Kang , Li Yanran 
Published: July 2021

AbstractStrigolactones (SLs) are a class of phytohormones playing diverse roles in plant growth and development, yet the limited access to SLs is largely impeding SL-based foundational investigations and applications. Here, we developedEscherichia coli-Saccharomyces cerevisiaeconsortia to establish a microbial biosynthetic platform for the synthesis of various SLs, including carlactone, carlactonic acid, 5-deoxystrigol (5DS, 6.65±1.71 µg/L), 4-deoxyorobanchol (4DO, 3.46±0.28 µg/L), and orobanchol (OB, 19.36±5.20 µg/L). The SL-producing platform enabled us to conduct functional identification of CYP722Cs from various plants as either OB or 5DS synthase. It also allowed us to quantitatively compare known variants of plant SL biosynthetic enzymes in the microbial system. The titer of 5DS was further enhanced through pathway engineering to 47.3 µg/L. This work provides a unique platform for investigating SL biosynthesis and evolution and lays the foundation for developing SL microbial production process.
Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKIIα-Cre mouse line
Authors: Matsuura Ken , Mohamed Haytham Mohamed Aly , Youssef Mohieldin Magdy Mahmoud , Yamamoto Tadashi 
Published: July 2021

AbstractThe CaMKIIα-Cre mouse line, one of the earliest established Cre driver lines, has resulted in over 800 papers to date. Here, we demonstrate that the second most widely used CaMKIIα-Cre line, Tg(Camk2a-cre)2Gsc (or CamiCre), shows ectopic overexpression of synaptotagmin 2, the most efficient Ca2+ sensor for fast synchronous neurotransmitter release, in excitatory presynapses of Cre+ brains. Moreover, RNA-seq analysis showed aberrant expression in Cre+ hippocampus, including upregulation of immediate early genes, such as Arc and Fos, and genes presumably derived from bacterial artificial chromosome transgene, such as Slc6a7. Most importantly, CamiCre+ mice showed functional phenotypes, such as hyperactivity and enhanced associative learning, suggesting neural activities are affected. These unexpected results suggest difficulties in interpreting results from studies using the CamiCre line and raise awareness of potential pitfalls in the use of Cre driver lines in general.
BOLD fMRI signals of visual white matter encode visuotopic information and predict effective connectivity between visual areas
Authors: Wang Huan , Wang Xiaoxiao , Wang Yanming , Zhang Du , Zhou Yifeng , Qiu Bensheng , Zhang Peng 
Published: July 2021

AbstractThe functional significance of BOLD signals in white matter (WM) remains unclear. The current study investigated whether 7T BOLD fMRI signal from visual WM tracts contains high fidelity retinotopic information and whether it correlates with the effective connectivity between visual areas. Population receptive field (pRF) analysis of the 7T retinotopy dataset from the Human Connectome Project revealed clear contralateral retinotopic representations from two visual WM bundles: optic radiation (OR) and vertical occipital fasciculus (VOF). The retinotopic organization of OR is consistent with post-mortem studies. The pRF size of WM voxels also increases with eccentricity. Based on the retinotopic maps of OR, we investigated whether BOLD signals in OR during visual stimulation are related to the resting-state effective connectivity between the lateral geniculate nucleus (LGN) and the primary visual cortex (V1). Results show that visually-evoked BOLD responses in OR correlate with the feedforward and feedback connectivity between the LGN and V1 during resting state. These findings demonstrate that WM BOLD signals contain high fidelity information such as visual field maps, and also predict the functional connectivity of brain areas.Significance statementWhite matter (WM) tracks conduct spiking activity between distant neurons. Weak fluctuations of BOLD signals in the WM can be detected with fMRI, but their functional relevance remains largely unknown. Here we characterized the visual field map properties of two major visual WM bundles: the optic radiation (OR) and vertical occipital fasciculus (VOF). Population receptive field analysis of the WM BOLD signals revealed clear visual field maps in both WM tracts. Effective connectivity analysis further showed that visually evoked BOLD responses in OR can predict the resting thalamo-cortical functional connectivity. These findings demonstrate that WM BOLD signals contain highly specific functional information and could directly index the functional connectivity between brain areas.
Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization
Authors: Kundinger Sean R. , Dammer Eric B. , Yin Luming , Hurst Cheyenne , Ping Lingyan , Duong Duc M. , Seyfried Nicholas T. 
Published: July 2021

ABSTRACTPost-translational modifications (PTMs) within splicing factor RNA-binding proteins (RBPs), such as phosphorylation, regulate several critical steps in RNA metabolism including spliceosome assembly, alternative splicing and mRNA export. Notably, the arginine-/serine-rich (RS) domains in SR proteins are densely modified by phosphorylation compared with the remainder of the proteome. Previously, we showed that dephosphorylation of SRSF2 regulated increased interactions with similar arginine-rich RBPs U1-70K and LUC7L3. In this work, we dephosphorylated nuclear extracts using phosphatase in vitro and analyzed equal amounts of detergent-soluble and -insoluble fractions by mass spectrometry-based proteomics. Correlation network analysis resolved 27 distinct modules of differentially soluble nucleoplasm proteins. We found classes of arginine-rich RBPs that decrease in solubility following dephosphorylation and enrich to the insoluble pelleted fraction, including the SR protein family and the SR-like LUC7L RBP family. Importantly, increased insolubility was not observed across broad classes of RBPs. Phosphorylation regulated SRSF2 structure, as dephosphorylated SRSF2 formed high molecular weight oligomeric species in vitro. Reciprocally, phosphorylation of SRSF2 by serine-/arginine protein kinase 2 (SRPK2) in vitro prevented high molecular weight SRSF2 species formation. Furthermore, we pharmacologically inhibited SRPKs in mammalian cells and observed increased cytoplasmic granules as well as the formation of cytoplasmic SRSF2 tubular structures that associate with microtubules by immunocytochemical staining. Collectively, these findings demonstrate that phosphorylation may be a critical modification that prevents arginine-rich RBP insolubility and oligomerization.
Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy
Authors: Huang Steven H. , Li Jiaruo , Fan Zhiyuan , Delgado Robert , Shvets Gennady 
Published: July 2021

AbstractInfrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures – plasmonic metasurfaces – as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.
Factors driving Thailand rice farmer decision-making in the choice of marketing channel
Authors: Thamthanakoon Nithicha , Huang Iona Yuelu , Eastham Jane , Ward Shane , Manning Louise 
Published: July 2021

PurposeSince the end of the latest rice-pledging scheme, Thai rice farmers have had more freedom in selecting marketing channels. Understanding the determinants of farmers' decision-making associated with these channels is of particular interest to multiple stakeholders in the rice value chain. This study aims to examine how economic, relational and psychological factors concurrently underpin Thai rice farmers' decision-making and influence their marketing channel choice.Design/methodology/approachDrawing on the theory of reasoned action and utility maximization of farmers’ decision making, this study used structural equation modeling to examine data collected from a nationwide sample of Thai rice farmers (n = 637), focusing on their past and intentional use of the three major marketing channels for paddy rice.FindingsThe determinants identified include four direct independent variables: attitude, subjective norm (social referents), transaction conditions and economic goals, and two indirect independent variables: past behavior and trust. Multi-group analysis suggests that rice co-operative users were more empowered to consider economic goals and attitude toward the channel, whilst rice miller and local collector users were more likely to be influenced by their social referents and the transaction conditions offered by the channel.Practical implicationsThe findings highlight the need for policy to address trust and transparency issues with intermediaries and to empower farmers through the improvement of market access.Originality/valueThe study makes a unique and substantive contribution to the knowledge of farmers' decision-making about marketing channel choice in Thailand and theoretically contributes to the indirect role of past behavior in predicting prospective intention.
Does organizational cronyism undermine social capital? Testing the mediating role of workplace ostracism and the moderating role of workplace incivility
Authors: Jawahar I.M. , Bilal Ahmad Raza , Fatima Tehreem , Mohammed Zach J. 
Published: July 2021

PurposeThe purpose of this study was to test the mediating influence of workplace ostracism in the relationship between organizational cronyism and social capital. In addition, the authors posited that workplace incivility would interact with cronyism to directly influence social capital, and indirectly through its effect on workplace ostracism.Design/methodology/approachUsing data collected in four waves, with a time interval of six weeks between each wave, from 358 university faculty members, the authors used the PROCESS macro to test the proposed moderated mediation model.FindingsResults indicated that workplace ostracism mediates the influence of organizational cronyism on social capital, and that workplace incivility interacts with cronyism to influence social capital directly, and indirectly through its effect on workplace ostracism.Originality/valueMost studies of social capital are theoretical. The few empirical studies have focused on factors that contribute to social capital and virtually no research exists on factors that undermine social capital. Based on the premise that negative interpersonal relationships are far more powerful in explaining outcomes relative to positive interpersonal relations and the notion that “bad is stronger than good,” the authors draw on social exchange theory and the norm of negative reciprocity to test a moderated mediation model and identify a mechanism and the condition under which social capital is undermined. The results have important implications for theory, research and practice.
Is Baidu index really powerful to predict the Chinese stock market volatility? New evidence from the internet information
Authors: Lang Qiaoqi , Wang Jiqian , Ma Feng , Huang Dengshi , Mohamed Ismail Mohamed Wahab 
Published: July 2021

PurposeThis paper verifies whether popular Internet information from Internet forum and search engine exhibit useful content for forecasting the volatility in Chinese stock market.Design/methodology/approachFirst, the authors’ study commences with several HAR-RV-type models, then the study amplifies them respectively with the posting volume and search frequency to construct HAR-IF-type and HAR-BD-type models. Second, from in-sample and out-of-sample analysis, the authors empirically investigate the interpretive ability, forecasting performance (statistic and economic). Third, various robustness checks are utilized to reconfirm the authors’ findings, including alternative forecast window, alternative evaluation method and alternative stock market. Finally, the authors further discuss the forecasting performance in different forecast horizons (h = 5, 10 and 20) and asymmetric effect of information from Internet forum.FindingsFrom in-sample perspective, the authors discover that posting volume exhibits better analytical ability for Chinese stock volatility than search frequency. Out-of-sample results indicate that forecasting models with posting volume could achieve a superior forecasting performance and increased economic value than competing models.Practical implicationsThese findings can help investors and decision-makers obtain higher forecasting accuracy and economic gains.Originality/valueThis study enriches the existing research findings about the volatility forecasting of stock market from two dimensions. First, the authors thoroughly investigate whether the Internet information could enhance the efficiency and accuracy of the volatility forecasting concerning with the Chinese stock market. Second, the authors find a novel evidence that the information from Internet forum is more superior to search frequency in volatility forecasting of stock market. Third, they find that this study not only compares the predictability of the posting volume and search frequency simply, but it also divides the posting volume into “good” and “bad” segments to clarify its asymmetric effect respectively.HighlightsThis study aims to verify whether posting volume and search frequency contain predictive content for estimating the volatility in Chinese stock market.The forecasting model with posting volume can achieve a superior forecasting performance and increases economic value than competing models.The results are robust in alternative forecast window, alternative evaluation method and alternative market index.The posting volume still can help to forecast future volatility for mid- and long-term forecast horizons. Additionally, the role of posting volume in forecasting Chinese stock volatility is asymmetric.
Multifunctional modified cotton fabric by one-step process using eco-friendly phytic acid and 1, 2, 3, 4-butanetetracarboxylic acid
Authors: Fang Yinchun , Liu Xinhua , Liu Hailong , Wang Qian 
Published: July 2021

PurposeCotton is one of the most common nature textile fiber that is widely used in clothing, bedding and decorative fields due to its comfort. However, the cellulosic cotton fiber has its own drawbacks. Cotton fiber belongs to flammable material with the limiting oxygen index (LOI) value about 18% that restricts its applications. Cotton fiber is easy to crease during the repeat wearing and laundering process that will influence the wearability. Therefore, it is very important to improve the flame retardancy and anticrease performance of cotton fabric.Design/methodology/approachIn this study, flame retardant and anticrease multifunctional modifications of cotton fabric were conducted by one-step pad–dry–cure process using eco-friendly phytic acid and 1, 2, 3, 4-butanetetracarboxylic acid.FindingsThe results of limited oxygen index (LOI) values and vertical burning test indicate that the flame retardancy of modified cotton fabric was greatly improved. The LOI value of modified cotton fabric reached 30.8% when the usage of phytic acid was 12%. The crease recovery angle was over 250° of the modified cotton fabric revealing good anticrease performance.Originality/valueThis research provides a novel feasible cost-effective one-step method for the multifunctional modified cellulosic fiber using eco-friendly chemical agents.
The impacts of income inequality on the gross domestic product under direct taxation: evidence from emerging market economies
Authors: Yıldırım Ahmet Eren , Dibo Mete 
Published: July 2021

PurposeThis study analyzes the impacts of income inequality after direct taxation on the gross domestic product as a fiscal policy tool in the development process.Design/methodology/approachThe model of the study is based on Munielo-Gallo and Roca-Sagales (2013), which examined the fiscal policy, income inequality and economic growth simultaneously. The study uses two models to analyze the relationship between income inequality and gross domestic production under direct taxation by employing autoregressive distributed lag (ARDL) model for selected emerging market economies.FindingEmpirical results reveal a negative long-run relationship between variables in some countries in line with the literature, despite a positive relationship in others. Moreover, the results exhibit the negative impact of income inequality after direct taxation on the gross domestic product decreases.Originality/valueResults of the study highlight the importance of direct taxation on income inequality concerning the reflects on economic growth. It suggests that when the income distribution is fairer, it may positively affect the gross domestic product. The study provides a new perspective to the related literature by investigating the role of income inequality under direct taxation for gross domestic product.