bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial extracellular electron transfer: a powerful route to the green biosynthesis of inorganic nanomaterials for multifunctional applications
Authors: Zou Long , Zhu Fei , Long Zhong-er , Huang Yunhong 
Published: April 2021

AbstractSynthesis of inorganic nanomaterials such as metal nanoparticles (MNPs) using various biological entities as smart nanofactories has emerged as one of the foremost scientific endeavors in recent years. The biosynthesis process is environmentally friendly, cost-effective and easy to be scaled up, and can also bring neat features to products such as high dispersity and biocompatibility. However, the biomanufacturing of inorganic nanomaterials is still at the trial-and-error stage due to the lack of understanding for underlying mechanism. Dissimilatory metal reduction bacteria, especially Shewanella and Geobacter species, possess peculiar extracellular electron transfer (EET) features, through which the bacteria can pump electrons out of their cells to drive extracellular reduction reactions, and have thus exhibited distinct advantages in controllable and tailorable fabrication of inorganic nanomaterials including MNPs and graphene. Our aim is to present a critical review of recent state-of-the-art advances in inorganic biosynthesis methodologies based on bacterial EET using Shewanella and Geobacter species as typical strains. We begin with a brief introduction about bacterial EET mechanism, followed by reviewing key examples from literatures that exemplify the powerful activities of EET-enabled biosynthesis routes towards the production of a series of inorganic nanomaterials and place a special emphasis on rationally tailoring the structures and properties of products through the fine control of EET pathways. The application prospects of biogenic nanomaterials are then highlighted in multiple fields of (bio-) energy conversion, remediation of organic pollutants and toxic metals, and biomedicine. A summary and outlook are given with discussion on challenges of bio-manufacturing with well-defined controllability.
The client satisfaction with device: a Rasch validation of the Arabic version in patients with upper and lower limb amputation
Authors: Bakhsh Hadeel R. , Kablan Nilüfer , Alammar Walaa , Tatar Yaşar , Ferriero Giorgio 
Published: April 2021

Abstract
Background
The Client Satisfaction with Devices (CSD) module of the Orthotics and Prosthetics Users’ Survey is an extensively used questionnaire that measures patients’ satisfaction with orthosis and prosthesis. However, the validated version for Arabic speakers (CSD-Ar) is only applicable for orthosis users.

Objectives
The aim of this study was to evaluate the psychometric proprieties of the CSD-Ar for prosthetics users.

Methods
The study used a convenience sample of prosthesis users from Saudi Arabia and Turkey (N = 183), who completed the CSD-Ar. The collected data were analysed using Rasch analysis to evaluate item fit, reliability indices, item difficulty, local item dependency, and differential item functioning (DIF) using WINSTEPS version 4.6.1.

Results
Based on the analysis, the four-response Likert-scale was acceptable, as shown by the category functioning test, All eight items did achieve a fit to the Rasch Model [(infit) and (outfit) mean-square 0.75 to 1.3]. Person separation reliability was 0.76, and item separation reliability was 0.94. A principal component analysis (PCA) showed satisfactory unidimensionality and no local item dependency. The DIF analysis showed no notable dependency among items on participant characteristics in terms of age, gender, duration of use, country, and level of amputation.

Conclusion
This study contributes to the confidence of using CSD-Ar to evaluate users’ satisfaction with different prostheses, affirming the need for further refinement of the quality of the outcome measure.
Job satisfaction of general practitioners: a cross-sectional survey in 34 countries
Authors: Stobbe Emiel J. , Groenewegen Peter P. , Schäfer Willemijn 
Published: April 2021

Abstract
Background
Job satisfaction of general practitioners (GPs) is important because of the consequences of low satisfaction for GPs, their patients and the health system, such as higher turnover, health problems for the physicians themselves, less satisfied patients, poor clinical outcomes and suboptimal health care delivery. In this study, we aim to explain differences in the job satisfaction of GPs within and between countries.

Methods
We performed a secondary analysis of cross-sectional survey data, collected between 2010 and 2012 on 7379 GPs in 34 (mostly European) countries, as well as data on country and health system characteristics from public databases. Job satisfaction is measured through a composite score of six items about self-reported job experience. Operationalisation of the theoretical constructs includes variables, such as the range of services GPs provide, working hours, employment status, and feedback from colleagues. Data were analysed using linear multilevel regression analysis, with countries and GPs as levels. We developed hypotheses on the basis of the Social Production Function Theory, assuming that GPs ‘produce’ job satisfaction through stimulating work that provides a certain level of comfort, adds to their social status and provides behavioural confirmation.

Results
Job satisfaction varies between GPs and countries, with high satisfaction in Denmark and Canada (on average 2.97 and 2.77 on a scale from 1–4, respectively) and low job satisfaction in Spain (mean 2.15) and Hungary (mean 2.17). One-third of the total variance is situated on the country level, indicating large differences between countries, and countries with a higher GDP per capita have more satisfied GPs. Health system characteristics are not related to GP job satisfaction. At the GP and practice level, performing technical procedures and providing preventive care, feedback from colleagues, and patient satisfaction are positively related to GP job satisfaction and working more hours is negatively related GP job satisfaction.

Conclusion
Overall and in terms of our theoretical approach, we found that GPs are able to ‘produce’ work-related well-being through activities and resources related to stimulation, comfort and behavioural confirmation, but not to status.
Astrocyte-specific expression of interleukin 23 leads to an aggravated phenotype and enhanced inflammatory response with B cell accumulation in the EAE model
Authors: Nitsch Louisa , Petzinna Simon , Zimmermann Julian , Schneider Linda , Krauthausen Marius , Heneka Michael T. , Getts Daniel R. , Becker Albert , Müller Marcus 
Published: April 2021

Abstract
Background
Interleukin 23 is a critical cytokine in the pathogenesis of multiple sclerosis. But the local impact of interleukin 23 on the course of neuroinflammation is still not well defined. To further characterize the effect of interleukin 23 on CNS inflammation, we recently described a transgenic mouse model with astrocyte-specific expression of interleukin 23 (GF-IL23 mice). The GF-IL23 mice spontaneously develop a progressive ataxic phenotype with cerebellar tissue destruction and inflammatory infiltrates with high amounts of B cells most prominent in the subarachnoid and perivascular space.

Methods
To further elucidate the local impact of the CNS-specific interleukin 23 synthesis in autoimmune neuroinflammation, we induced a MOG35-55 experimental autoimmune encephalomyelitis (EAE) in GF-IL23 mice and WT mice and analyzed the mice by histology, flow cytometry, and transcriptome analysis.

Results
We were able to demonstrate that local interleukin 23 production in the CNS leads to aggravation and chronification of the EAE course with a severe paraparesis and an ataxic phenotype. Moreover, enhanced multilocular neuroinflammation was present not only in the spinal cord, but also in the forebrain, brainstem, and predominantly in the cerebellum accompanied by persisting demyelination. Thereby, interleukin 23 creates a pronounced proinflammatory response with accumulation of leukocytes, in particular B cells, CD4+ cells, but also γδ T cells and activated microglia/macrophages. Furthermore, transcriptome analysis revealed an enhanced proinflammatory cytokine milieu with upregulation of lymphocyte activation markers, co-stimulatory markers, chemokines, and components of the complement system.

Conclusion
Taken together, the GF-IL23 model allowed a further breakdown of the different mechanisms how IL-23 drives neuroinflammation in the EAE model and proved to be a useful tool to further dissect the impact of interleukin 23 on neuroinflammatory models.
Auto-classification of biomass through characterization of their pyrolysis behaviors using thermogravimetric analysis with support vector machine algorithm: case study for tobacco
Authors: Yin Chao , Deng Xiaohua , Yu Zhiqiang , Liu Zechun , Zhong Hongxiang , Chen Ruting , Cai Guohua , Zheng Quanxing , Liu Xiucai , Zhong Jiawei , Ma Pengfei , He Wei , Lin Kai , Li Qiaoling , Wu Anan 
Published: April 2021

Abstract
Background
During the biomass-to-bio-oil conversion process, many studies focus on studying the association between biomass and bio-products using near-infrared spectra (NIR) and chemical analysis methods. However, the characterization of biomass pyrolysis behaviors using thermogravimetric analysis (TGA) with support vector machine (SVM) algorithm has not been reported. In this study, tobacco was chosen as the object for biomass, because the cigarette smoke (including water, tar, and gases) released by tobacco pyrolysis reactions decides the sensory quality, which is similar to biomass as a renewable resource through the pyrolysis process.

Results
SVM algorithm has been employed to automatically classify the planting area and growing position of tobacco leaves using thermogravimetric analysis data as the information source for the first time. Eighty-eight single-grade tobacco samples belonging to four grades and eight categories were split into the training, validation, and blind testing sets. Our model showed excellent performances in both the training and validation set as well as in the blind test, with accuracy over 91.67%. Throughout the whole dataset of 88 samples, our model not only provides precise results on the planting area of tobacco leave, but also accurately distinguishes the major grades among the upper, lower, and middle positions. The error only occurs in the classification of subgrades of the middle position.

Conclusions
From the case study of tobacco, our results validated the feasibility of using TGA with SVM algorithm as an objective and fast method for auto-classification of tobacco planting area and growing position. In view of the high similarity between tobacco and other biomasses in the compositions and pyrolysis behaviors, this new protocol, which couples the TGA data with SVM algorithm, can potentially be extrapolated to the auto-classification of other biomass types.
Ameliorative role of SIRT1 in peritoneal fibrosis: an in vivo and in vitro study
Authors: Guo Yanhong , Wang Liuwei , Gou Rong , Wang Yulin , Shi Xiujie , Zhang Yage , Pang Xinxin , Tang Lin 
Published: April 2021

Abstract
Background
Peritoneal fibrosis is one of the major complications induced by peritoneal dialysis (PD). Damaged integrity and function of peritoneum caused by peritoneal fibrosis not only limits the curative efficacy of PD and but affects the prognosis of patients. However, the detailed mechanisms underlying the process remain unclear and therapeutic strategy targeting TGF‐β is deficient. Transforming growth factor‐β (TGF‐β) signaling participates in the progression of peritoneal fibrosis through enhancing mesothelial-mesenchymal transition of mesothelial cells.

Methods
The study aims to demonstrate the regulatory role of Sirtuin1 (SIRT1) to the TGF‐β signaling mediated peritoneal fibrosis. SIRT1−/− mice were used to establish animal model. Masson’s staining and peritoneal equilibration assay were performed to evaluate the degree of peritoneal fibrosis. QRT-PCR assays were used to estimate the RNA levels of Sirt1 and matrix genes related to peritoneal fibrosis, and their protein levels were examined by Western blot assays.

Results
SIRT1 significantly decreased in vivo post PD treatment. SIRT1 knockout exacerbated peritoneal fibrosis both in vivo and vitro. Overexpression of SIRT1 efficiently inhibited peritoneal fibrosis by inhibiting the peritoneal inflammation and the activation of TGF‐β signaling.

Conclusion
SIRT1 ameliorated peritoneal fibrosis both in vivo and in vitro through inhibiting the expression of protein matrix induced by TGF‐β signaling.
Determinants of adolescent substance use in Africa: a systematic review and meta-analysis protocol
Authors: Jumbe Sandra , Kamninga Tony Mwenda , Mwalwimba Isaac , Kalu Ukwuori-Gisela 
Published: April 2021

Abstract
Background
Adolescent substance use continues to be a growing major public health concern in Africa. Recent studies infer an overall estimated prevalence of 42% among adolescents in sub-Saharan Africa. Unfortunately, this phenomenon is not adequately documented across many settings in the continent despite known negative health and social consequences on affected individuals and their communities. Little is known about the social context of substance use in Africa among this population. Our aim is to conduct a systematic review, exploring the determinants and associated factors that influence adolescent substance use in Africa.

Methods
We will search the following databases (from January 2000 onwards): PubMed, the Cochrane Library, African Journals Online (AJOL), Google Scholar, ScienceDirect and the World Health Organization (WHO) African Index Medicus. We will include population-based observational studies reporting on determinants and/or risk factors of substance use among adolescents (age 10–19 years) across Africa. Two reviewers will independently screen all citations, full-text articles and abstract data. Potential conflicts will be resolved through discussion. Study methodological quality (or bias) will be appraised using appropriate tools. If feasible, we will conduct a random-effects meta-analysis of data. We plan to conduct a meta-synthesis of qualitative studies where appropriate

Discussion
This review will describe the range of determinants and associated factors found to significantly influence adolescent substance use in Africa over the last two decades. Documenting this evidence is important as it can potentially inform comprehensive interventions and treatment programmes that are targeted at adolescents and their parents in these settings.

Systematic review registration
PROSPERO CRD42020190158
Compressible flow simulation with moving
geometries using the Brinkman penalization in high-order Discontinuous
Galerkin
Authors: Ebrahimi Pour Neda , Anand Nikhil , Klimach Harald , Roller Sabine 
Published: April 2021

AbstractIn this work we investigate the Brinkman volume penalization technique in
the context of a high-order Discontinous Galerkin method to model moving wall boundaries
for compressible fluid flow simulations. High-order approximations are especially of
interest as they require few degrees of freedom to represent smooth solutions
accurately. This reduced memory consumption is attractive on modern computing systems
where the memory bandwidth is a limiting factor. Due to their low dissipation and
dispersion they are also of particular interest for aeroacoustic problems. However, a
major problem for the high-order discretization is the appropriate representation of
wall geometries. In this work we look at the Brinkman penalization technique, which
addresses this problem and allows the representation of geometries without modifying the
computational mesh. The geometry is modelled as an artificial porous medium and embedded
in the equations. As the mesh is independent of the geometry with this method, it is not
only well suited for high-order discretizations but also for problems where the
obstacles are moving. We look into the deployment of this strategy by briefly discussing
the Brinkman penalization technique and its application in our solver and investigate
its behavior in fundamental one-dimensional setups, such as shock reflection at a moving
wall and the formation of a shock in front of a piston. This is followed by the
application to setups with two and three dimensions, illustrating the method in the
presence of curved surfaces.
Comparing different response time threshold setting methods to detect low effort on a large-scale assessment
Authors: Soland James , Kuhfeld Megan , Rios Joseph 
Published: April 2021

AbstractLow examinee effort is a major threat to valid uses of many test scores. Fortunately, several methods have been developed to detect noneffortful item responses, most of which use response times. To accurately identify noneffortful responses, one must set response time thresholds separating those responses from effortful ones. While other studies have compared the efficacy of different threshold-setting methods, they typically do so using simulated or small-scale data. When large-scale data are used in such studies, they often are not from a computer-adaptive test (CAT), use only a handful of items, or do not comprehensively examine different threshold-setting methods. In this study, we use reading test scores from over 728,923 3rd–8th-grade students in 2056 schools across the United States taking a CAT consisting of nearly 12,000 items to compare threshold-setting methods. In so doing, we help provide guidance to developers and administrators of large-scale assessments on the tradeoffs involved in using a given method to identify noneffortful responses.
Immigrant optimism or immigrant pragmatism? Linguistic capital, orientation towards science and occupational expectations of adolescent immigrants
Authors: Sikora Joanna , Pokropek Artur 
Published: April 2021

Abstract
Background
Studies demonstrate that occupational optimism can boost adolescents’ academic attainment and perseverance in education. To contribute to this literature, we consider two hypotheses. The first posits that bilingual immigrants are remarkably resourceful and determined. Thus, they are more occupationally ambitious than their peers. The second proposes that immigrant students engage in “strategic adaptation” by specializing in science, viewed as a level playing field.

Methods
To assess these hypotheses at two points of time, we analyze data from 19 societies that participated in the Programme for International Student Assessment (PISA) in 2006 and 2015. Our primary method is path analysis with balanced replicate weights (BRR) undertaken separately for each country’s data.

Results
We find that, in many countries, bilingual immigrants expect to enter higher status occupations than non-immigrants. However, immigrants who do not speak another language are also optimistic, so linguistic resources cannot explain occupational ambition. Furthermore, immigrants accord science more instrumental value and enjoy it more at school, which accounts, across societies, for up to 12% of the variation in vocational optimism indicated by the expected occupational status, and up to 41% in plans to pursue a career in science professions.

Conclusion
Our results align with the “strategic adaptation” argument that many young immigrants might seek to specialize in science as a pragmatic tactic to ensure high occupational attainment.
Postsurgical oral symptoms after insertion of one or two implants for mandibular overdentures: short-term results of a randomized clinical trial
Authors: de Resende Gabriela Pereira , Dias Ana Paula , Leles José Luiz Rodrigues , de Souza João Antônio Chaves , Leles Cláudio Rodrigues 
Published: April 2021

Abstract
Objective
This randomized clinical trial aimed to compare the short-term postsurgical symptoms after insertion of one or two implants for retention of a mandibular overdenture. This study investigated whether the less invasive single-implant approach results in lower postoperative symptoms compared to the conventional two-implant overdenture.

Materials and methods
Patients received new complete dentures and were randomly assigned to groups receiving one or two single-stage, early-loaded hydrophilic implants, inserted in the midline (n = 23), or the lateral incisor-canine area bilaterally (n = 24). Patient-reported postoperative symptoms were measured in a 0–100 visual analogue scale concerning pain in the surgical area, pain when chewing, bleeding, swelling, and unpleasantness. Data collection occurred 24 h and 7 and 21 days after surgery. Demographic and clinical features (smoking habit, classification of the residual ridges, and mucosal width and thickness at the implant sites), osteotomy for alveolar bone reduction, and surgery time were tested as predictors of symptom levels.

Results
Overall reported symptoms were mild and self-limited, with high rates of complete remission after the early loading period of 3 weeks. Progressive improvement of symptoms occurred from the 24-h to the 7-day and 21-day follow-ups (p < 0.001), similarly in both groups. None of the clinical predictors was significantly associated with the changes in symptoms.

Conclusions
Findings suggest that the insertion of one or two implants may result in similar postoperative outcomes.

Clinical relevance
The severity of short-term postoperative symptoms may not be a critical factor for the decision between overdenture treatment with one or two implants.
Association between CCN1 gene polymorphism and acute coronary syndrome in Chinese Han and Uygur populations
Authors: Li Yan-Hong , Luo Jun-Yi , Fang Bin-Bin , Du Guo-Li , Tian Ting , Liu Fen , Li Xiao-Mei , Yang Yi-Ning 
Published: April 2021

Abstract
Background
CCN1 plays a crucial role in the modulation of cardiovascular diseases. However, whether CCN1 genetic variants are involved in the susceptibility of ACS remains unknown. Hence, the present study investigates the association between CCN1 polymorphisms and ACS among Han and Uygur populations in Xinjiang, China.

Results
In this case-control study, 1234 Han (547 ACS patients and 687 controls) and 932 Uygur (471 ACS patients and 461 controls) were genotyped using SNPscanTM for three single-nucleotide polymorphisms (SNPs, rs6576776, rs954353, and rs3753794) of the human CCN1 gene. In the Uygur population, we found that the detected frequencies of the C allele (25.3% vs. 18.3%, P<0.001) and CC genotype (6.4% vs. 3.0%, P=0.001) of rs6576776 were significantly higher in the ACS patients than in the control participants. Differences in rs6576776 regarding the dominant model (CC+CG vs. GG, 44.2% vs. 55.8%, P=0.001) and the recessive model (CC vs. CG+GG, 6.4% vs. 93.6%, P=0.016) were observed between the two groups. The frequencies of the GGC and AGC haplotypes in those with ACS were significantly higher than those in the control group (all P<0.05) in the Uygur population. After adjusting for hypertension, diabetes, lipids and smoking, all of which indicate that the rs6576776 C allele is associated with higher risk of ACS (odds ratio (OR)=1.798, 95% confidence interval (CI), 1.218-2.656, P=0.003). In Han population, neither the distribution of genotypes and alleles of the CCN1 gene three SNPs nor the distribution of haplotypes constructed with the three SNPs exhibited a significant difference between the ACS patients and control participants.

Conclusions
Our study document that the CCN1 gene rs6576776 C allele is associated with higher susceptibility of ACS and that the frequencies of GGC and AGC haplotypes are higher among the Uygur ACS patients.