bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endovascular Therapeutic Hypothermia Adjunctive To Percutaneous Coronary Intervention in Acute Myocardial Infarction: Realistic Simulation as a Game Changer
Authors: Dallan Luis Augusto Palma , Dae Michael , Giannetti Natali Schiavo , Polastri Tathiane Facholi , Lima Marian Keiko Frossard , Rochitte Carlos Eduardo , Hajjar Ludhmila Abrahao , Martin Claudia Yanet Bernoche San , Lima Felipe Gallego , Nicolau Jose Carlos , de Oliveira Mucio Tavares , Dallan Luis Alberto Oliveira , Silva Expedito Eustaquio Ribeiro da , Filho Roberto Kalil , Neto Pedro Alves Lemos , Timerman Sergio 
Published: October 2021

Abstract
Background: Endovascular therapeutic hypothermia (ETH) reduces the damage by ischemia/reperfusion cell syndrome in cardiac arrest and has been studied as an adjuvant therapy to percutaneous coronary intervention (PCI) in ST-elevation myocardial infarction (STEMI). New available advanced technology allows cooling much faster, but there is paucity of resources for training to avoid delays in door-to-balloon time (DTB) due to ETH and subsequently coronary reperfusion, which would derail the procedure. The aim of the study was to describe the process for the development of a simulation, training & educational protocol for the multidisciplinary team to perform optimized ETH as an adjunctive therapy for STEMI. Methods and Results: We developed an optimized simulation protocol using modern mannequins in different realistic scenarios for the treatment of patients undergoing ETH adjunctive to PCI for STEMIs starting from the emergency room, through the CathLab, and to the intensive care unit (ICU) using the Proteus® Endovascular System. The primary endpoint was door-to-balloon (DTB) time. We successfully trained 361 multidisciplinary professionals in realistic simulation using modern mannequins and sham situations in divisions of the hospital where real patients would be treated. The focus of simulation and training was logistical optimization and educational debriefing with strategies to reduce waste of time in patient's transportation from different departments, and avoiding excessive rewarming during transfer. Conclusions: Realistic simulation, intensive training and educational debriefing for the multidisciplinary team propitiated feasible endovascular therapeutic hypothermia as an adjuvant therapy to primary PCI in STEMI. ClinicalTrials.gov: NCT02664194.
Raman Spectrum of Follicular Fluid: a Potential Biomarker for Oocyte Developmental Competence in Polycystic Ovary Syndrome
Authors: Huang Xin , Hong Ling , Wu Yuanyuan , Chen Miaoxin , Kong Pengcheng , Ruan Jingling , Teng Xiaoming , Wei Zhiyun 
Published: October 2021

Abstract
Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in reproductive women where abnormal folliculogenesis is considered as a common characteristic. Our aim is to evaluate the potential of follicular fluid (FF) Raman spectra to predict oocyte development and pregnancy outcome, so as to prioritize the best promising oocyte for implantation, reducing both physiological and economical burdens of PCOS patients. In addition, the altered metabolic profiles will be identified to explore the aetiology and pathobiology of PCOS. Methods: In this study, follicular fluid samples obtained from 150 PCOS and 150 non-PCOS women were measured with Raman spectroscopy. Individual Raman spectrum was analyzed to find biologic components contributing to the occurrence of PCOS. More importantly, the Raman spectra of follicular fluid from the 150 PCOS patients were analyzed via machine-learning algorithms to evaluate their predictive value for oocyte development potential and clinical pregnancy. Results: Mean-centered Raman spectra and principal component analysis showed global differences in the footprints of follicular fluid between PCOS and non-PCOS women. Two Raman zones (993-1,165 cm-1 and 1,439-1,678cm-1) were identified for describing the largest variances between the two groups, with the former higher and the latter lower in PCOS FF. The tentative assignments of corresponding Raman bands included phenylalanine and β -carotene. Moreover, it was found that FF, in which oocytes would develop into high-quality blastocysts and obtain high clinical pregnancy rate, were detected with lower quantification of the integration at 993-1,165 cm-1 and higher quantification of the integration at 1,439-1,678 cm-1 in PCOS. In addition, based on Raman spectra of PCOS FF, the machine-learning algorithms via the fully connected artificial neural network (ANN) achieved the overall accuracies of 90% and 74% in correctly assigning oocyte developmental potential and clinical pregnancy, respectively. Conclusions: The study suggests that the PCOS displays unique metabolic profiles in follicular fluid which could be detected by Raman spectroscopy. Specific bands in Raman spectra have the biomarker potential to predict the oocyte development and pregnancy outcome for PCOS patients. Importantly, these data may provide some valuable biochemical information and metabolic signatures that will help us to understand the abnormal follicular development in PCOS.
An automated framework for high-throughput predictions of
NMR chemical shifts within liquid solutions
Authors: Atwi Rasha , Chen Ying , Han Kee Sung , Mueller Karl , Murugesan Vijayakumar , Rajput Nav Nidhi 
Published: October 2021

Abstract
Identifying stable speciation in multicomponent liquid solutions is of fundamental importance to areas ranging from electrochemistry to organic chemistry and biomolecular systems. However, elucidating this complex solvation environment is a daunting task even when using advanced experimental and computational techniques. Here, we introduce a fully automated, high-throughput computational framework for the accurate and robust prediction of stable species present in liquid solutions by computing the nuclear magnetic resonance (NMR) chemical shifts of molecules. The framework automatically extracts and categorizes hundreds of thousands of atomic clusters from classical molecular dynamics (CMD) simulations to identify the most stable speciation in the solution and calculate their NMR chemical shifts via DFT calculations. Additionally, the framework creates an output database of computed chemical shifts for liquid solutions across a wide chemical and parameter space. This task can be infeasible experimentally and challenging using conventional computational methods. To demonstrate the capabilities of our framework, we compare our computational results to experimental measurements for a complex test case of magnesium bis(trifluoromethanesulfonyl)imide Mg(TFSI)2 salt in dimethoxyethane (DME) solvent, which is a common electrolyte system for Mg-based batteries. Our extensive benchmarking and analysis of the Mg2+ solvation structural evolutions reveal critical factors such as the effect of force field parameters that influence the accuracy of NMR chemical shift predictions in liquid solutions. Furthermore, we show how the framework reduces the efforts of performing and managing over 300 13C and 600 1H DFT chemical shift predictions to a single submission procedure. By enabling more efficient and accurate high-throughput computations of NMR chemical shifts, our approach can accelerate theory-guided design of liquid solutions for various applications.
Detection and Quantification of EBV, CMV and HHV-6 DNA in Stomach Frozen Tissue of Gastritis and Gastric Cancer Patients
Authors: Sarshari Behrang , Mohebbi Seyed Reza , Ravanshad Mehrdad , Shahrokh Shabnam , Asadzadeh-Aghdaei Hamid , Zali Mohammad Reza 
Published: October 2021

Abstract
BackgroundHuman herpes viruses (HHVs) are among the most common infectious agents detected in the gastrointestinal tract that might be involved in oncogenesis and other gastrointestinal disorders. Although the link between the EBV and gastric cancer (GC) has been established, role of the viruses in various stomach diseases remain unknown. MethodsThe frequencies and viral copy number of EBV, CMV and HHV-6 among 50 GC tumors and 105 gastritis tissues were measured by quantitative real-time PCR.ResultsEBV, CMV and HHV-6 DNA were detected in 30 (60%), 7 (14%) and 9 (18%) of the tumor specimens, while in gastritis patients were 20 (19%), 13 (12.3%) and 16 (15.2%) respectively. The EBV frequency was significantly higher in GC compared with gastritis patients (p<0.0001). Viral load in both GC and gastritis groups was higher in either tumor or gastritis lesion compared with matched adjacent normal tissue. This study showed a clear association between GC with both EBV (p=0.0268) and CMV (p=0.0012). In addition, analyzes revealed a strong association between the EBV, CMV and HHV-6 with gastritis (p=0.0026, p <0.0001 and p=0.0405 respectively). ConclusionsOur results suggest that these three viruses might contribute to induction and development the gastritis and GC.
Theoretical Investigation into the Solvent Effect on the Thermal Decomposition of RDX in Tetrahydrofuran, Acetone, Toluene and Benzene
Authors: Ren Fu-de , Cao Xiong , Cui Yun-tong 
Published: October 2021

Abstract
In order to clarify the solvent effect on the thermal decomposition of explosive, the N–NO2 trigger-bond strengths and ring strains of RDX (cyclotrimethylenetrinitramine) in its H-bonded complexes with solvent molecules (i.e., tetrahydrofuran, acetone, toluene and benzene), and the activation energies of the intermolecular hydrogen exchanges between the solvent molecules and C3H8O2N4 or CH4O2N2, as the model molecule of RDX, were investigated by the BHandHLYP, B3LYP, MP2(full) and M06-2X methods with the 6-311++G(2df,2p) basis set, accompanied by a comparison with the calculations by the integral equation formalism polarized continuum model. The solvent effects ignore the ring strain while strengthen the N–NO2 bond, leading to a decreased sensitivity, as is opposite to the experimental results. However, the activation energies are in the order of C3H8O2N4/CH4O2N2∙∙∙acetone < C3H8O2N4/CH4O2N2∙∙∙THF < C3H8O2N4/CH4O2N2∙∙∙toluene < C3H8O2N4/CH4O2N2∙∙∙benzene < C3H8O2N4/CH4O2N2, suggesting that the order of the critical explosion temperatures should be RDX∙∙∙acetone < RDX∙∙∙THF < RDX∙∙∙toluene < RDX∙∙∙benzene < RDX, as is roughly consistent with the experimental results. Therefore, the intermolecular hydrogen exchange with the HONO elimination is the essence of the solvent effect on the thermal decomposition of RDX. The solvent effect is confirmed by reduced density gradient, atoms in molecules and surface electrostatic potentials.
The Use of Intrathecal Morphine for Acute Postoparative Pain in Lower Limb Arthroplasty Surgery: A Survey of Practice at an Academic Hospital.
Authors: Sibanyoni Mpumelelo , Biyase Ntombiyethu , Chakane Palesa Motshabi 
Published: October 2021

Abstract
Background and purpose of the study: Intrathecal morphine (ITM) provides optimal postoperative analgesia in patients who are scheduled for total knee and hip operation with spinal anaesthesia. However, the ideal dose at which maximal analgesic effect occurs with minimal side effects is not known. This study aimed to describe the use of two doses of ITM, and side effect profile in patients undergoing elective hip and knee arthroplasty.Methods: This was a prospective, descriptive, and contextual study conducted on patients who had total hip and knee replacement at Chris Hani Baragwanath Academic Hospital from 1 September to 30 November 2020. The sample size consisted of 66 patients who were 18 years and older, American Society of Anaesthesiology (ASA) classification 1-3, patients who had received either 100 mcg or 150 mcg ITM dose under spinal anaesthesia and sent to the ward postoperatively. Visual Analogue Scale (VAS) score was used to assess pain in the first 24 hours, consumption of rescue analgesia and reported side effects were documented.Results: There was no relationship between age, weight, ASA classification or type of surgery and VAS score classification groups. Patients who received 100 mcg ITM had a higher median VAS pain score 2 (1-5) compared to those who received 150 mcg ITM 1 (0-2), p = 0.01. The need for rescue analgesia between the two groups was marginally less in the 150mcg ITM group (p =0.098). There was no difference in the rate of side effects between the 100 mcg ITM group [12 (41%)] and the 150 mcg ITM group [17 (59%)], p = 0.92. Rescue analgesia was marginally different between groups, p = 0.09. There were no real differences in the VAS pain scores between the total knee and total hip surgeries. None of the patients experienced clinically significant respiratory depression. Conclusion: The 150mcg ITM dose provided good analgesic effects with longer duration of action and comparable side effect profile to the 100mcg ITM dose. This dose was not associated with development of respiratory depression can therefore be administered safely to patients who are discharged to the ward postoperatively in a resource constraint environment.
Criteria For The Development of Green Office Standard of Thailand For Environmental Sustainability
Authors: Aroonsrimorakot Sayam , Laiphrakpam Meena , Sarapirom Kornkanok 
Published: October 2021

Abstract
The Green Office Standard was developed to lessen resource consumption and wastage in the office work process, achieve savings and minimize CO2 emission from the office's operations in the environment. Its long-term goal is training and motivating staff for resource efficiency, energy conservation, reduce pollution and wastage in the offices’ work process and recycle the generated waste and these behavioral changes will lead to environmental sustainability. This research aims to develop a Green Office Standard in Thailand explaining its criteria to promote the establishment of green offices all over Thailand. Specifically, this article describes the criteria of green office standards, which are grouped into six categories as 1) Policies determination, operation planning, and continual improvement; 2) Communication and creating awareness; 3) Resource and energy consumption; 4) Office waste management; 5. Environment and safety; and 6) Eco-friendly procurement. It used mixed research methodology in different stages from March 2016 to August 2018, but mainly by the application of EDFR (Ethnographic Delphi Futures Research), by selecting a group of 17 expert panels to obtain their outlook and opinions. Green Office Standard was finally developed to certify participant-organizations in Thailand that conforms their management according to the criteria of Green Office Management Standard.
Gastric Side Effects and the Stomach Dosimetric Analysis in Left-Sided Breast Cancer Radiotherapy in Free-Breathing and Deep Inspiration Breath-Hold Technique
Authors: Yang Dong , Piao Ying , Yuan Fengshun , Chen Hongtao , Zhang Ding , Li Xianming 
Published: October 2021

Abstract
Background
Adjuvant radiotherapy following surgery reduces the local recurrence and improves the prognosis. However, a considerable part of patients developed digestive reaction in daily treatment. In order to explore the correlation between breast radiotherapy and gastric toxicity, we investigated the clinic symptoms and stomach dose during DIBH or FB mode while left-sided breast cancer patients (LSBCP) receiving radiotherapy.
Methods
In the study, 124 LSBCP received adjuvant radiotherapy after surgery at our department were analyzed clinical characteristics and enquired about gastrointestinal side effects after treatment. Moreover, dosimetric parameters were assessed.
Results
There was no statistically significant difference between the two groups in age, T staging, N staging, hormone receptors, human epidermal receptor-2 (HER2), surgical methods, fractionated regimen, and chemotherapy conditions. Stomach volumes, the use of the DIBH gating technique (FB/DIBH), and higher fractionated dose (Dmax/F) were associated with a statistically significantly greater risk for acute radiotherapy toxicity.
Conclusion
In order to cut down gastric side effects after breast radiotherapy, large meals should be avoided before treatment. DIBH treatment should be implemented in centers where conditions are satisfied to reduce radiotherapy side effects. Furthermore, dose limitation in stomach should be considered when the radiotherapy plan was formulated, especially for the patients treated with hypofractionated radiotherapy.
Sleep Quality and BMI in Pregnancy– A Prospective Cohort Study
Authors: Tang Yafang , Dai Fei , Razali Nurul Syaza , Tagore Shephali , Chern Bernard SM , Tan Kok Hian 
Published: October 2021

Abstract
Background: Pregnancy associated sleep disturbance is a common pregnancy-related complication which can lead to significant maternal distress and adverse pregnancy outcomes. Sleep quality can be affected by multiple factors and high BMI or obesity has been recognized as one of them. Various previous studies have demonstrated poorer sleep quality during pregnancy. However, most studies included assessment at only one point of pregnancy. This prospective cohort study aimed to better evaluate the effect of pregnancy on the quality of sleep throughout the antenatal period and how BMI affects antenatal sleep.Methods: A total of 926 women were recruited before 14 weeks of gestation and followed throughout pregnancy. The Pittsburgh Sleep Quality Index questionnaire was employed to assess sleep quality in 4 antenatal visits throughout pregnancy. Their weight was also recorded at each visit.Results: The PSQI global score was higher towards the later part of pregnancy (6.4 to 8.0, p<0.001) and highest at the 4th visit. Sleep latency was longer as pregnancy progressed (18.5 mins to 23.2 mins, p=0.001). Sleep duration became shorter over time and was the shortest at the 4th visit (7.1 hours to 6.5 hours, p<0.001). Sleep efficiency was the lowest at the 4th visit (85.2% to 81.6%, p<0.001). The same trend was observed for subjects in different BMI groups throughput pregnancy. PSQI score increased and sleep duration decreased as BMI increased. The effect of increasing BMI on PSQI and sleep duration was only observed in the higher BMI groups (>25kg/m2).Conclusions: Our study showed that sleep quality gradually declined throughout pregnancy for all BMI groups. Higher BMI was associated with poorer sleep as represented by PSQI score and sleep duration, particularly in the high BMI subgroups.
Genotyping of Chlamydia Abortus Using Multiple Loci Variable Number of Tandem Repeats Analysis Technique
Authors: Barati Sara , Bakhtiari Naghmeh Moori , Shokoohizadeh Leili , Gorbanpoor Masoud , Momtaz Hassan 
Published: October 2021

Abstract
Background: The correlation between various factors (geographical region, clinical incidence, and host type) and the genomic heterogeneity has been shown in several bacterial strains including Chlamydia abortus. Methods: The aim of this study was to survey the predominant types of C. abortus strains isolated from ruminants in Iran by the multiple loci variable number of tandem repeats (VNTR) analysis (MLVA) method. C. abortus infection was evaluated in a total of 117 aborted fetuses by real-time PCR. The isolation was done via the inoculation of the positive samples in chicken embryo and the L929 cell line. Genotyping was carried out by MLVA typing technique. Results: Forty samples (34.2%) were detected with C. abortus infection; however, chlamydial infection in ruminants of Charmahal/Bakhtiari (3 bovines and 35 sheep) was higher than that of Khuzestan (2 sheep). All MLVA types (MT1-MT8) were detected in the collected samples from Charmahal/Bakhtiari but only 2 types (MT1 and MT3) were reported in samples from Khuzestan. The main MT type was MT1 (32% of samples). Although in this study only 9 cow samples were investigated, they possessed similar clusters to those obtained from sheep (MT1 and MT6).Variation of type in sheep samples (MT1 to MT8) was more than that of bovine samples (MT1, and MT6); this can be attributed to the fact that more samples of sheep were studied as against the bovine samples. Conclusion: Although the difference between the detected same MT types in several animal species or between 2 geographic areas is significant, comprehensive studies are still needed. In Iran, due to traditional intercourse between animals of different provinces, the spread of other types is typically highly probable.
“Beneficial Role of Skim Milk Against Drug Resistant Escherichia Coli Associated With Pediatric Diarrhoea"
Authors: Chellapandi Karuppasamy , Subbarayan Sarathbabu , De Mandal Surajit , Ralte Lalsanglura , Kumar Nachimuthu Senthil , Dutta Tapan Kumar , Sharma Indu 
Published: October 2021

Abstract

Background: Antibiotic-resistance in E. coli is a global issue affecting humans especially the pediatric population. Antibiotic-resistant E. coli is a pathogen frequently pediatric population as well as healthy adults. Methods: This study aimed to examine the antibiotic resistance of E. coli causing pediatric diarrhea and its drug-resistant rates, its adhering abilities to cell line in vitro, and inhibition efficiency of a few selected chemical compounds. Clinical strains were isolated from both the healthy and infected pediatric population of Mizoram. Results: Adhesion is a significant pathogenic process during bacterial infections, which has been employed for pathotyping of DEC by comparing adhesion efficiency in both normal (CHO-k1) and cancer (HeLa) cell lines. E. coli adherent pathotypes were identified by both PCR assay and in-vitro cell adhesion assays; the study also evaluated the adhesion inhibition ability of human skimmed milk, gentamicin, and cephalexin in-vitro. Of all isolates, 20.05% of adherent DEC (EPEC, DAEC, and EIEC) and 11.39 % of non-adherent DEC (STEC and ETEC) were found to be associated with pediatric diarrhoea in Mizoram. Human skimmed milk has a high potential adhesion inhibition against EAEC (50.25/90.90 µg/mL), EPEC (53.42/259.70 µg/mL), and EIEC (59.13/30.30 µg/mL) in both cell lines in comparison with gentamicin and cephalexin. Conclusion: This study concludes that as a dietary supplement- human skimmed milk has high potential to prevent adhesion of DEC pathotypes in cells in-vitro thus in in-vivo.
Facial Expression and Electrodermal Activity Analysis for Continuous Pain Intensity Monitoring On The X-ITE Pain Database
Authors: Othman Ehsan , Werner Philipp , Saxen Frerk , Al-Hamadi Ayoub , Gruss Sascha , Walter Steffen 
Published: October 2021

Abstract
Automatic systems enable continuous monitoring of patients' pain intensity as shown in prior studies. Facial expression and physiological data such as electrodermal activity (EDA) are very informative for pain recognition. The features extracted from EDA indicate the stress and anxiety caused by different levels of pain. In this paper, we investigate using the EDA modality and fusing two modalities (frontal RGB video and EDA) for continuous pain intensity recognition with the X-ITE Pain Database. Further, we compare the performance of automated models before and after reducing the imbalance problem in heat and electrical pain datasets that include phasic (short) and tonic (long) stimuli. We use three distinct real-time methods: A Random Forest (RF) baseline methods [Random Forest classifier (RFc) and Random Forest regression (RFr)], Long-Short Term Memory Network (LSTM), and LSTM using sample weighting method (called LSTM-SW). Experimental results (1) report the first results of continuous pain intensity recognition using EDA data on the X-ITE Pain Database, (2) show that LSTM and LSTM-SW outperform guessing and baseline methods (RFc and RFr), (3) confirm that the electrodermal activity (EDA) with most models is the best, (4) show the fusion of the output of two LSTM models using facial expression and EDA data (called Decision Fusion = DF). The DF improves results further with some datasets (e.g. Heat Phasic Dataset (HTD)).