bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental influences on sinking rates and distributions of transparent exopolymer particles after a typhoon surge at the Western Pacific
Authors: Islam M. Shahanul , Sun Jun , Zhang Guicheng , Chen Zhuo , Zhou Hui 
Published: May 2021

AbstractA multidisciplinary approach was used to investigate the causes of the distributions and sinking rates of transparent exopolymer particles (TEPs) during the period of September–October (2017) in the Western Pacific Ocean (WPO); the study period was closely dated to a northwest typhoon surge. The present study discussed the impact of biogeophysical features on TEPs and their sinking rates (sTEP) at depths of 0–150 m. During the study, the concentration of TEPs was found to be higher in areas adjacent to the Kuroshio current and in the bottom water layer of the Mindanao upwelling zone due to the widespread distribution of cyanobacteria, i.e., Trichodesmium hildebrandti and T. theibauti. The positive significant regressions of TEP concentrations with Chl-a contents in eddy-driven areas (R2 = 0.73, especially at 100 m (R2 = 0.75)) support this hypothesis. However, low TEP concentrations and TEPs were observed at mixed layer depths (MLDs) in the upwelling zone (Mindanao). Conversely, high TEP concentrations and high sTEP were found at the bottom of the downwelling zone (Halmahera). The geophysical directions of eddies may have caused these conditions. In demonstrating these relations, the average interpretation showed the negative linearity of TEP concentrations with TEPs (R2 = 0.41 ~ 0.65) at such eddies. Additionally, regression curves (R2 = 0.78) indicated that atmospheric pressure played a key role in the changes in TEPs throughout the study area. Diatoms and cyanobacteria also curved the TEPs significantly (R2 = 0.5, P < 0.05) at the surface of the WPO. This study also revealed that TEP concentration contributes less to the average particulate organic carbon in this oligotrophic WPO.
Magnetic and structural properties of the solid solution CuAl2(1−x)Ga2xO4
Authors: Bullard T. J. , Susner M. A. , Taddei K. M. , Brant J. A. , Haugan T. J. 
Published: May 2021

AbstractCuAl2O4 is a ternary oxide spinel with Cu2+ ions ($$s=1/2$$

s
=
1
/
2

) primarily populating the A-site diamond sublattice. The compound is reported to display evidence of spin glass behavior but possess a non-frozen magnetic ground state below the transition temperature. On the other hand, the spinel CuGa2O4 displays spin glass behavior at ~ 2.5 K with Cu2+ ions more readily tending to the B-site pyrochlore sublattice. Therefore, we investigate the magnetic and structural properties of the solid solution CuAl2(1-x)Ga2xO4 examining the evolution of the magnetic behavior as Al3+ is replaced with a much larger Ga3+ ion. Our results show that the Cu2+ ions tend to migrate from tetrahedral to octahedral sites as the Ga3+ ion concentration increases, resulting in a concomitant change in the glassy magnetic properties of the solution. Results indicate glassy behavior for much of the solution with a general trend towards decreasing magnetic frustration as the Cu2+ ion shifts to the B-site. However, the $$x=0.1$$

x
=
0.1

and 0.2 members of the system do not show glassy behavior down to our measurement limit (1.9 K) suggesting a delayed spin glass transition. We suggest that these two members are additional candidates for investigation to access highly frustrated exotic quantum states.
Heat stress and immune response phenotype affect DNA methylation in blood mononuclear cells from Holstein dairy cows
Authors: Livernois A. M. , Mallard B. A. , Cartwright S. L. , Cánovas A. 
Published: May 2021

AbstractHeat stress negatively affects health and production in cows. Examining the cellular response to heat stress could reveal underlying protective molecular mechanisms associated with superior resilience and ultimately enable selection for more resilient cattle. This type of investigation is increasingly important as future predictions for the patterns of heat waves point to increases in frequency, severity, and duration. Cows identified as high immune responders based on High Immune Response technology (HIR) have lower disease occurrence compared to their average and low immune responder herd-mates. In this study, our goal was to identify epigenetic differences between high and low immune responder cows in response to heat stress. We examined genome-wide DNA methylation of blood mononuclear cells (BMCs) isolated from high and low cows, before and after in vitro heat stress. We identified differential methylation of promoter regions associated with a variety of biological processes including immune function, stress response, apoptosis, and cell signalling. The specific differentially methylated promoter regions differed between samples from high and low cows, and results revealed pathways associated with cellular protection during heat stress.
Response to perturbation during quiet standing resembles delayed state feedback optimized for performance and robustness
Authors: Zelei Ambrus , Milton John , Stepan Gabor , Insperger Tamas 
Published: May 2021

AbstractPostural sway is a result of a complex action–reaction feedback mechanism generated by the interplay between the environment, the sensory perception, the neural system and the musculation. Postural oscillations are complex, possibly even chaotic. Therefore fitting deterministic models on measured time signals is ambiguous. Here we analyse the response to large enough perturbations during quiet standing such that the resulting responses can clearly be distinguished from the local postural sway. Measurements show that typical responses very closely resemble those of a critically damped oscillator. The recovery dynamics are modelled by an inverted pendulum subject to delayed state feedback and is described in the space of the control parameters. We hypothesize that the control gains are tuned such that (H1) the response is at the border of oscillatory and nonoscillatory motion similarly to the critically damped oscillator; (H2) the response is the fastest possible; (H3) the response is a result of a combined optimization of fast response and robustness to sensory perturbations. Parameter fitting shows that H1 and H3 are accepted while H2 is rejected. Thus, the responses of human postural balance to “large” perturbations matches a delayed feedback mechanism that is optimized for a combination of performance and robustness.
Transient incubation of cultured hippocampal neurons in the absence of magnesium induces rhythmic and synchronized epileptiform-like activity
Authors: Mele Miranda , Vieira Ricardo , Correia Bárbara , De Luca Pasqualino , Duarte Filipe V. , Pinheiro Paulo S. , Duarte Carlos B. 
Published: May 2021

AbstractCell culture models are important tools to study epileptogenesis mechanisms. The aim of this work was to characterize the spontaneous and synchronized rhythmic activity developed by cultured hippocampal neurons after transient incubation in zero Mg2+ to model Status Epilepticus. Cultured hippocampal neurons were transiently incubated with a Mg2+-free solution and the activity of neuronal networks was evaluated using single cell calcium imaging and whole-cell current clamp recordings. Here we report the development of synchronized and spontaneous [Ca2+]i transients in cultured hippocampal neurons immediately after transient incubation in a Mg2+-free solution. Spontaneous and synchronous [Ca2+]i oscillations were observed when the cells were then incubated in the presence of Mg2+. Functional studies also showed that transient incubation in Mg2+-free medium induces neuronal rhythmic burst activity that was prevented by antagonists of glutamate receptors. In conclusion, we report the development of epileptiform-like activity, characterized by spontaneous and synchronized discharges, in cultured hippocampal neurons transiently incubated in the absence of Mg2+. This model will allow studying synaptic alterations contributing to the hyperexcitability that underlies the development of seizures and will be useful in pharmacological studies for testing new drugs for the treatment of epilepsy.
Electromechanical impedance based self-diagnosis of piezoelectric smart structure using principal component analysis and LibSVM
Authors: Jiang Xie , Zhang Xin , Tang Tao , Zhang Yuxiang 
Published: May 2021

AbstractThe long-term use of a piezoelectric smart structure make it difficult to judge whether the structure or piezoelectric lead zirconate titanate (PZT) is damaged when the signal changes. If the sensor fault occurs, the cases and degrees of the fault are unknown based on the electromechanical impedance method. Therefore, after the principal component analysis (PCA) of six characteristic indexes, a two-component solution that could explain 99.2% of the variance in the original indexes was obtained to judge whether the damage comes from the PZT. Then LibSVM was used to make an effective identification of four sensor faults (pseudo soldering, debonding, wear, and breakage) and their three damage degrees. The result shows that the identification accuracy of damaged PZT reached 97.5%. The absolute scores of PCA comprehensive evaluation for structural damages are less than 0.5 while for sensor faults are greater than 0.6. By comparing the scores of the samples under unknown conditions with the set threshold, whether the sensor faults occur is effectively judged; the intact and 12 possible damage states of PZT can be all classified correctly with the model trained by LibSVM. It is feasible to use LibSVM to classify the cases and degrees of sensor faults.
Cytogenetic markers using single-sequence probes reveal chromosomal locations of tandemly repetitive genes in scleractinian coral Acropora pruinosa
Authors: Vacarizas Joshua , Taguchi Takahiro , Mezaki Takuma , Okumura Masatoshi , Kawakami Rei , Ito Masumi , Kubota Satoshi 
Published: May 2021

AbstractThe short and similar sized chromosomes ofAcroporapose a challenge for karyotyping. Conventional methods, such as staining of heterochromatic regions, provide unclear banding patterns that hamper identification of such chromosomes. In this study, we used short single-sequence probes from tandemly repetitive 5S ribosomal RNA (rRNA) and core histone coding sequences to identify specific chromosomes ofAcropora pruinosa. Both the probes produced intense signals in fluorescence in situ hybridization, which distinguished chromosome pairs. The locus of the 5S rDNA probe was on chromosome 5, whereas that of core histone probe was on chromosome 8. The sequence of the 5S rDNA probe was composed largely of U1 and U2 spliceosomal small nuclear RNA (snRNA) genes and their interspacers, flanked by short sequences of the 5S rDNA. This is the first report of a tandemly repetitive linkage of snRNA and 5S rDNA sequences in Cnidaria. Based on the constructed tentative karyogram and whole genome hybridization, the longest chromosome pair (chromosome 1) was heteromorphic. The probes also hybridized effectively with chromosomes of otherAcroporaspecies and population, revealing an additional core histone gene locus.We demonstrated the applicability of short-sequence probes as chromosomal markers with potential for use across populations and species ofAcropora.
Energetic context determines the effects of multiple upwelling-associated stressors on sea urchin performance
Authors: Murie Kindall A. , Bourdeau Paul E. 
Published: May 2021

AbstractGlobally, kelp forests are threatened by multiple stressors, including increasing grazing by sea urchins. With coastal upwelling predicted to increase in intensity and duration in the future, understanding whether kelp forest and urchin barren urchins are differentially affected by upwelling-related stressors will give insight into how future conditions may affect the transition between kelp forests and barrens. We assessed how current and future-predicted changes in the duration and magnitude of upwelling-associated stressors (low pH, dissolved oxygen, and temperature) affected the performance of purple sea urchins (Strongylocentrotus purpuratus) sourced from rapidly-declining bull kelp (Nereocystis leutkeana) forests and nearby barrens and maintained on habitat-specific diets. Kelp forest urchins were of superior condition to barrens urchins, with ~ 6–9 times more gonad per body mass. Grazing and condition in kelp forest urchins were more negatively affected by distant-future and extreme upwelling conditions, whereas grazing and survival in urchins from barrens were sensitive to both current-day and all future-predicted upwelling, and to increases in acidity, hypoxia, and temperature regardless of upwelling. We conclude that urchin barren urchins are more susceptible to increases in the magnitude and duration of upwelling-related stressors than kelp forest urchins. These findings have important implications for urchin population dynamics and their interaction with kelp.
Disruption of the odorant coreceptor Orco impairs foraging and host finding behaviors in the New World screwworm fly
Authors: Paulo Daniel F. , Junqueira Ana C. M. , Arp Alex P. , Vieira André S. , Ceballos Jorge , Skoda Steven R. , Pérez-de-León Adalberto A. , Sagel Agustin , McMillan William O. , Scott Maxwell J. , Concha Carolina , Azeredo-Espin Ana M. L. 
Published: May 2021

AbstractThe evolution of obligate ectoparasitism in blowflies (Diptera: Calliphoridae) has intrigued scientists for over a century, and surprisingly, the genetics underlying this lifestyle remain largely unknown. Blowflies use odors to locate food and oviposition sites; therefore, olfaction might have played a central role in niche specialization within the group. In insects, the coreceptor Orco is a required partner for all odorant receptors (ORs), a major gene family involved in olfactory-evoked behaviors. Hence, we characterized the Orco gene in the New World screwworm, Cochliomyia hominivorax, a blowfly that is an obligate ectoparasite of warm-blooded animals. In contrast, most of the closely related blowflies are scavengers that lay their eggs on dead animals. We show that the screwworm Orco orthologue (ChomOrco) is highly conserved within Diptera, showing signals of strong purifying selection. Expression of ChomOrco is broadly detectable in chemosensory appendages, and is related to morphological, developmental, and behavioral aspects of the screwworm biology. We used CRISPR/Cas9 to disrupt ChomOrco and evaluate the consequences of losing the OR function on screwworm behavior. In two-choice assays, Orco mutants displayed an impaired response to floral-like and animal host-associated odors, suggesting that OR-mediated olfaction is involved in foraging and host-seeking behaviors in C. hominivorax. These results broaden our understanding of the chemoreception basis of niche occupancy by blowflies.
Degradation of soil quality by the waste leachate in a Mediterranean semi-arid ecosystem
Authors: Yeilagi Sh. , Rezapour Salar , Asadzadeh F. 
Published: May 2021

AbstractThe assessment of soil quality indices in waste leachate-affected soils is vital to understand the threats of land quality degradation and how to control it. In this respect, a study was conducted on the effects of uncontrolled landfill leachate on soil quality index (SQI) in calcareous agricultural lands using 28 soil variables. Using the total data set (TDS) and minimum data set (MDS) approaches, the SQI was compared between leachate-affected soils (LAS) and control soils by the integrated quality index (IQI) and nemoro quality index (NQI) methods. The results revealed that LAS were significantly enriched by soil salinity-sodicity indices including electrical conductivity (EC), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP), fertility indices including total N, available P and K, organic carbon, and cation exchange capacity (CEC), exchangeable cations (Ca, Mg, K, and Na), the available and total fractions of heavy metals (Zn, Cu, Cd, Pb, Ni). After the leachate got its way into the soil, the values of IQI and NQI were dropped ranging 5–16% and 6.5–13% for the TDS approach and 5–15.2% and 7.5–12.2 for the MDS approach, respectively. Clearly, the data showed that soil quality degradation was encouraged and stimulated by the leachate. Among the different models of SQI applied in the present study, IQI determined by MDS was the optimal model to estimate soil quality and predict crop yields given the analysis of the correlations among the SQI models, the correlations between the SQI models and wheat yield, and sensitivity index values.
Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells
Authors: Tsuneishi Ruri , Saku Noriaki , Miyata Shoko , Akiyama Saeko , Javaregowda Palaksha Kanive , Ite Kenta , Takashima Nagisa , Toyoda Masashi , Kimura Tohru , Kuroda Masahiko , Nakazawa Atsuko , Kasahara Mureo , Nonaka Hidenori , Kamiya Akihide , Kiyono Tohru , Yamauchi Junji , Umezawa Akihiro 
Published: May 2021

AbstractAmmonia has a cytotoxic effect and can therefore be used as a selection agent for enrichment of zone I hepatocytes. However, it has not yet been determined whether ammonia-treated hepatocyte-like cells are able to proliferate in vitro. In this study, we employed an ammonia selection strategy to purify hepatocyte-like cells that were differentiated from human embryonic stem cells (ESCs) and from induced pluripotent stem cells (iPSCs). The resistance to cytotoxicity or cell death by ammonia is likely attributable to the metabolism of ammonia in the cells. In addition to ammonia metabolism-related genes, ammonia-selected hepatocytes showed increased expression of the cytochrome P450 genes. Additionally, the ammonia-selected cells achieved immortality or at least an equivalent life span to human pluripotent stem cells without affecting expression of the liver-associated genes. Ammonia treatment in combination with in vitro propagation is useful for obtaining large quantities of hepatocytes.
Pharmacodynamic evaluation of suppression of in vitro resistance in Acinetobacter baumannii strains using polymyxin B-based combination therapy
Authors: Fedrigo Nayara Helisandra , Shinohara Danielle Rosani , Mazucheli Josmar , Nishiyama Sheila Alexandra Belini , Carrara-Marroni Floristher Elaine , Martins Frederico Severino , Zhu Peijuan , Yu Mingming , Sy Sherwin Kenneth B. , Tognim Maria Cristina Bronharo 
Published: May 2021

AbstractThe emergence of polymyxin resistance in Gram-negative bacteria infections has motivated the use of combination therapy. This study determined the mutant selection window (MSW) of polymyxin B alone and in combination with meropenem and fosfomycin against A. baumannii strains belonging to clonal lineages I and III. To evaluate the inhibition of in vitro drug resistance, we investigate the MSW-derived pharmacodynamic indices associated with resistance to polymyxin B administrated regimens as monotherapy and combination therapy, such as the percentage of each dosage interval that free plasma concentration was within the MSW (%TMSW) and the percentage of each dosage interval that free plasma concentration exceeded the mutant prevention concentration (%T>MPC). The MSW of polymyxin B varied between 1 and 16 µg/mL for polymyxin B-susceptible strains. The triple combination of polymyxin B with meropenem and fosfomycin inhibited the polymyxin B-resistant subpopulation in meropenem-resistant isolates and polymyxin B plus meropenem as a double combination sufficiently inhibited meropenem-intermediate, and susceptible strains. T>MPC 90% was reached for polymyxin B in these combinations, while %TMSW was 0 against all strains. TMSW for meropenem and fosfomycin were also reduced. Effective antimicrobial combinations significantly reduced MSW. The MSW-derived pharmacodynamic indices can be used for the selection of effective combination regimen to combat the polymyxin B-resistant strain.
Barriers and potential solutions for improved surgical care for children with hernia in Eastern Uganda
Authors: Ajiko Mary Margaret , Löfgren Jenny , Ekblad Solvig 
Published: May 2021

AbstractFive billion people lack timely, affordable access to surgery. A large proportion of these are children. Qualitative research investigating the barriers to surgical care for children and ways of overcoming them is lacking. This study focused on children with hernia, a very common paediatric surgical condition for which surgery is the only effective treatment. The main aim of this qualitative study was to explore barriers to surgical care for children and identify potential solutions. Data were collected from parents of children with hernia and from health care providers at Soroti Regional Referral Hospital in eastern Uganda. Parents’ experiences, motives and barriers when accessing care were explored. The health care providers’ knowledge, perceptions and practices relating to children with hernia were investigated. The data were analysed using thematic content analysis. Traditional beliefs and gender inequality were considered major issues. Possible solutions included partnering with the local community in efforts to increase knowledge and acceptability in the community in general and by parents in particular. A formation of a surgical team dedicated to the management of children with surgical conditions was suggested as way to improve quality and increase volume of surgery for children.
Effective phase noise considerations in magnon based parametric excitations
Authors: Venugopal Aneesh , Victora R. H. 
Published: May 2021

AbstractMagnon-phase is an important entity in the parametric processes involving magnons, yet the general qualitative and quantitative consequences of the phase-noise on nonlinear properties remain far from understood. In the current simulation-based theoretical study, we explore the direct impact the phase-noise has on non-linearity. We use analytical techniques usually employed in the study of hydrodynamics to explain the magnon-based nonlinear phenomena. The behavior of the threshold-field and growth rate of the magnons in the presence of Gaussian phase-noise is analytically predicted. These predictions are verified by micromagnetic simulations. Such results are of crucial importance in the design and engineering of both traditional and futuristic devices.
Distinct microbial community along the chronic oil pollution continuum of the Persian Gulf converge with oil spill accidents
Authors: Rezaei Somee Maryam , Dastgheib Seyed Mohammad Mehdi , Shavandi Mahmoud , Ghanbari Maman Leila , Kavousi Kaveh , Amoozegar Mohammad Ali , Mehrshad Maliheh 
Published: May 2021

AbstractThe Persian Gulf, hosting ca. 48% of the world’s oil reserves, has been chronically exposed to natural oil seepage. Oil spill studies show a shift in microbial community composition in response to oil pollution; however, the influence of chronic oil exposure on the microbial community remains unknown. We performed genome-resolved comparative analyses of the water and sediment samples along Persian Gulf’s pollution continuum (Strait of Hormuz, Asalouyeh, and Khark Island). Continuous exposure to trace amounts of pollution primed the intrinsic and rare marine oil-degrading microbes such as Oceanospirillales, Flavobacteriales, Alteromonadales, and Rhodobacterales to bloom in response to oil pollution in Asalouyeh and Khark samples. Comparative analysis of the Persian Gulf samples with 106 oil-polluted marine samples reveals that the hydrocarbon type, exposure time, and sediment depth are the main determinants of microbial response to pollution. High aliphatic content of the pollution enriched for Oceanospirillales, Alteromonadales, and Pseudomonadales whereas, Alteromonadales, Cellvibrionales, Flavobacteriales, and Rhodobacterales dominate polyaromatic polluted samples. In chronic exposure and oil spill events, the community composition converges towards higher dominance of oil-degrading constituents while promoting the division of labor for successful bioremediation.