bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological variation and reference change value data for serum copper, zinc and selenium in Turkish adult population
Authors: Bal Ceylan , Erdogan Serpil , Gök Gamze , Nural Cemil , Özbek Betül , Ercan Müjgan , Serdar Muhittin , Erel Özcan 
Published: April 2021

AbstractObjectivesCalculation of biological variation (BV) components is very important in evaluating whether a test result is clinically significant. The aim of this study is to analyze BV components for copper, zinc and selenium in a cohort of healthy Turkish participants.MethodsA total of 10 serum samples were collected from each of the 15 healthy individuals (nine female, six male), once a week, during 10 weeks. Copper, zinc and selenium levels were analyzed by atomic absorption spectrometer. BV parameters were calculated with the approach suggested by Fraser.ResultsAnalytical variation (CVA), within-subject BV (CVI), between-subject BV (CVG) values were 8.4, 7.1 and 4.3 for copper; 4.2, 9.1 and 13.7 for zinc; 7.6, 2.5 and 6.9 for selenium, respectively. Reference change values (RCV) were 30.46, 27.56 and 22.16% for copper, zinc and selenium, respectively. The index of individuality (II) values were 1.65, 0.66 and 0.36 for copper, zinc and selenium, respectively.ConclusionsAccording to the results of this study, traditional reference intervals can be used for copper but we do not recommend using it for zinc and selenium. We think that it would be more accurate to use RCV value for zinc and selenium in terms of following significant changes in recurrent results of a patient.
A McMaster retrospective: how publishing in a student journal shaped my career
Authors: Morris Alan 
Published: April 2021

Alan G. Morris is Professor in the Department of Human Biology at the University of Cape Town. A Canadian by birth and upbringing, Professor Morris is also a naturalised South African. He has an undergraduate degree in Biology from Wilfrid Laurier University in Waterloo Ontario, and a PhD in Anatomy from the University of the Witwatersrand in Johannesburg. Professor Morris has published extensively on the origin of anatomically modern humans, and the Later Stone Age, Iron Age and Historic populations of Kenya, Malawi, Namibia and South Africa. In more recent years he has extended his skeletal biology knowledge to the field of forensic anthropology. Professor Morris’ book ‘Missing and Murdered’ was the winner of the WW Howells Prize for 2013 from the American Anthropological Association. He has an additional interest in South African history and has published on the history of race classification, the history of physical anthropology in South Africa and on the Canadian involvement in the Anglo-Boer War. Professor Morris was selected as a visiting Fulbright Scholar in 2012-2013 and spent 9 months at The Ohio State University where he worked with American scholars on the ‘Global History of Health’ project. He is a council member of the Van Riebeeck Society for the Publication of Southern African Historical Documents, an associate editor of the South African Journal of Science and an elected member of the Academy of Science of South Africa.
Ideology, Agency, and the Federal Acknowledgement Process
Authors: Barron Nicholas 
Published: April 2021

In June of 2013, the Assistant Secretary-Indian Affairs, Kevin Washburn, began holding tribal consultations in an attempt to reform the Federal Acknowledgement Process (FAP) with the input of recognized and unrecognized indigenous peoples. Between June and September of 2013, unacknowledged Californian Indian groups, including the Amah Mustun Tribal Band of Ohlone/Costanoan Indians (Amah Mutsun), the Ohlone/Costanoan Esselen Nation (Esselen), and the Muwekma Ohlone Indian Tribe (Muwekma), submitted separate letters in an attempt to voice their concerns and recommendations. This situation offers a useful case study for anthropologists attempting to study the role of ideology within the context of federal recognition. Using Phil Abrams theorization of the state as a historically determined and processual formation in conjunction with Louis Althusser’s discussion of ideology and Sherry Ortner’s conceptualization of agency, I discursively analyze each comment letter with special attention paid to discourses of history. With this approach, I make three interrelated arguments. First, the FAP is an inherently contradictory ideological project of the state that produces a paradoxical narrative of indigenous history. Second, the historical narratives within these letters reflect an incomplete and contested process of interpellation that seeks to reify state power through the reproduction of hegemonic ideas. Third, these historical discourses reflect the different political strategies of representation that unacknowledged peoples formulate to contest the process of interpellation as they navigate a paradoxical state ideology. Ultimately, these conclusions point towards the incomplete, dialectical, and contested nature of state ideology within the system of federal recognition.
Mis/representation & Silence: Gendered Sex Work Discourse in the London Free Press
Authors: Dawthorne Nathan 
Published: April 2021

In December 2014, despite the Supreme Court of Canada finding Canada's prostitution laws unconstitutional, the Conservative government passed a bill criminalizing the buying of sex and the advertisement of sex for sale. Sex work has a long history as a hot-button topic, and it continues to remain newsworthy throughout the country. This public discussion in some contexts has privileged certain lobbyists and so-called advocates, disregarding or distorting the voices of sex workers themselves. This territory is starkly heteronormative, reinforcing gendered stereotypes and naturalizing certain types of heterosexual behaviour while ignoring a spectrum of other realities. By analysizing depictions of sex work published for 2013 in the London Free Press, a politically centre-right newspaper printed in a midsized Canadian city, this paper provides analysis of articles about sex work in the local-regional context of London, Ontario (Canada). Exposing a Foucauldian rarefaction of discourse, the analysis works to unveil ideological underpinnings, fleshing out a distorted gendered discourse.
Capital Conversion in the Organized Crime of the Favelas
Authors: Gutierrez Adriana 
Published: April 2021

Segregated in the hills of Rio de Janeiro, favelas are socially and economically marginalized slums, with pervasive drug crime. As a result of limited government intervention, drug lords assume the mandate in these sectors, reinforcing poverty and social exclusion.Traditional approaches to poverty analyze this context with capital scarcity as a point of reference. Moreover, the concept of capital has been used to denounce structural inequalities that are reproduced in social classes. By the same token, it is argued that the accumulation of capital may lead to social mobility. Low-income neighborhoods have their own resources and forms of mobilization. Conditions of precariousness can be explored without focusing on the absence of resources, but rather on the ways in which local capital gets mobilized and converted. In the favelas, drug gangs have their own capital dynamics that make them acquire and retain control over the territory. In this paper, I examine how capital conversion and mobilization among members of organized crime in these districts of Rio de Janeiro reinforce structural inequalities by perpetuating social exclusion.
Considerations for Chemotherapy Treatment in Platinum Resistant High-Grade Serous Ovarian Cancer
Authors: Reintjes Caitlin Marie 
Published: April 2021

Ovarian cancer is considered to be the most fatal type of any gynecological cancer. Prognosis for the disease is poor, with a median survival of only thirty-two months following diagnosis and a five-year survival rate of only 39%. Many of the most lethal ovarian cancer cases are classified as part of the high-grade serous ovarian cancer (HGSOC) subtype, which is the most aggressive form of the disease. The primary concern with regards to treatment is that nearly 30% of patients will develop a resistance to forms of platinum chemotherapy, which is the main method of treatment. This suggests that a one-size fits all approach cannot be taken to treat ovarian cancer, and that further research must be done to understand how to treat the patients who present with platinum resistance. This literature review examines the mutations within two susceptible loci, specifically, the p53 and BRCA1/2 genes, in order to understand how platinum resistance develops and why it is present in some patients. The objectives of this review are to characterize the underlying genetic mechanisms affecting platinum resistance, specify the biomarkers associated with those mechanisms, and describe alternative methods for approaching the treatment of ovarian cancer on an individual scale.
Roller-Integrated Compaction Assessment of Earth-Rock Dam Materials Considering Operation Modes
Authors: Liu Donghai , Wang Youle , Chen Junjie , Liang Jianyu 
Published: April 2021

Abstract
An earth-rock dam is composed of multiple materials compacted by rollers with complex operation modes, which can easily lead to the abnormal fluctuation of harmonic-ratio-based roller-integrated compaction measurement values (HR-RICMVs). Without proper handling, the abnormal fluctuation would result in unreliable assessment of compaction quality. As an attempt to address the issue of abnormal fluctuation, this study presented an improved regression model for roller-integrated compaction assessment of earth-rock dam materials. The proposed model considered the effect of vibration frequency on HR-RICMVs under different operation modes. The applicability of the improved model was verified by an analysis of correlation and reliability in different earth-rock dam materials. The results indicate that the model has significant merits in the assessment of compaction quality under complex operation modes. This research provides a potential way for the compaction quality control of earth-rock dam materials based on HR-RICMVs in future studies.
Predictive Visual Motion Extrapolation Emerges Spontaneously and without Supervision at Each Layer of a Hierarchical Neural Network with Spike-Timing-Dependent Plasticity
Authors: Burkitt Anthony N. , Hogendoorn Hinze 
Published: April 2021

The fact that the transmission and processing of visual information in the brain takes time presents a problem for the accurate real-time localization of a moving object. One way this problem might be solved is extrapolation: using an object's past trajectory to predict its location in the present moment. Here, we investigate how a simulatedin silicolayered neural network might implement such extrapolation mechanisms, and how the necessary neural circuits might develop. We allowed an unsupervised hierarchical network of velocity-tuned neurons to learn its connectivity through spike-timing-dependent plasticity (STDP). We show that the temporal contingencies between the different neural populations that are activated by an object as it moves causes the receptive fields of higher-level neurons to shift in the direction opposite to their preferred direction of motion. The result is that neural populations spontaneously start to represent moving objects as being further along their trajectory than where they were physically detected. Because of the inherent delays of neural transmission, this effectively compensates for (part of) those delays by bringing the represented position of a moving object closer to its instantaneous position in the world. Finally, we show that this model accurately predicts the pattern of perceptual mislocalization that arises when human observers are required to localize a moving object relative to a flashed static object (the flash-lag effect; FLE).SIGNIFICANCE STATEMENTOur ability to track and respond to rapidly changing visual stimuli, such as a fast-moving tennis ball, indicates that the brain is capable of extrapolating the trajectory of a moving object to predict its current position, despite the delays that result from neural transmission. Here, we show how the neural circuits underlying this ability can be learned through spike-timing-dependent synaptic plasticity and that these circuits emerge spontaneously and without supervision. This demonstrates how the neural transmission delays can, in part, be compensated to implement the extrapolation mechanisms required to predict where a moving object is at the present moment.
Memory Destabilization and Reconsolidation Dynamically Regulate the PKMζ Maintenance Mechanism
Authors: Bernabo Matteo , Haubrich Josue , Gamache Karine , Nader Karim 
Published: April 2021

Useful memory must balance between stability and malleability. This puts effective memory storage at odds with plasticity processes, such as reconsolidation. What becomes of memory maintenance processes during synaptic plasticity is unknown. Here we examined the fate of the memory maintenance protein PKMζ during memory destabilization and reconsolidation in male rats. We found that NMDAR activation and proteasome activity induced a transient reduction in PKMζ protein following retrieval. During reconsolidation, new PKMζ was synthesized to re-store the memory. Failure to synthesize new PKMζ during reconsolidation impaired memory but uninterrupted PKMζ translation was not necessary for maintenance itself. Finally, NMDAR activation was necessary to render memories vulnerable to the amnesic effect of PKMζ-antisense. These findings outline a transient disruption and renewal of the PKMζ memory maintenance mechanism during plasticity. We argue that dynamic changes in PKMζ protein levels can serve as an exemplary model of the molecular changes underlying memory destabilization and reconsolidation.SIGNIFICANCE STATEMENTMaintenance of long-term memory relies on the persistent activity of PKMζ. However, after retrieval, memories can become transiently destabilized and must be reconsolidated within a few hours to persist. During this period of plasticity, what happens to maintenance processes, such as those involving PKMζ, is unknown. Here we describe dynamic changes to PKMζ expression during both destabilization and reconsolidation of auditory fear memory in the amygdala. We show that destabilization induces a NMDAR- and proteasome-dependent loss of synaptic PKMζ and that reconsolidation requires synthesis of new PKMζ. This work provides clear evidence that memory destabilization disrupts ongoing synaptic maintenance processes which are restored during reconsolidation.
Engram Size Varies with Learning and Reflects Memory Content and Precision
Authors: Leake Jessica , Zinn Raphael , Corbit Laura H. , Fanselow Michael S. , Vissel Bryce 
Published: April 2021

Memories are rarely acquired under ideal conditions, rendering them vulnerable to profound omissions, errors, and ambiguities. Consistent with this, recent work using context fear conditioning has shown that memories formed after inadequate learning time display a variety of maladaptive properties, including overgeneralization to similar contexts. However, the neuronal basis of such poor learning and memory imprecision remains unknown. Using c-fos to track neuronal activity in male mice, we examined how these learning-dependent changes in context fear memory precision are encoded in hippocampal ensembles. We found that the total number of c-fos-encoding cells did not correspond with learning history but instead more closely reflected the length of the session immediately preceding c-fos measurement. However, using a c-fos-driven tagging method (TRAP2mouse line), we found that the degree of learning and memory specificity corresponded with neuronal activity in a subset of dentate gyrus cells that were active during both learning and recall. Comprehensive memories acquired after longer learning intervals were associated with more double-labeled cells. These were preferentially reactivated in the conditioning context compared with a similar context, paralleling behavioral discrimination. Conversely, impoverished memories acquired after shorter learning intervals were associated with fewer double-labeled cells. These were reactivated equally in both contexts, corresponding with overgeneralization. Together, these findings provide two surprising conclusions. First, engram size varies with learning. Second, larger engrams support better neuronal and behavioral discrimination. These findings are incorporated into a model that describes how neuronal activity is influenced by previous learning and present experience, thus driving behavior.SIGNIFICANCE STATEMENTMemories are not always formed under ideal circumstances. This is especially true in traumatic situations, such as car accidents, where individuals have insufficient time to process what happened around them. Such memories have the potential to overgeneralize to irrelevant situations, producing inappropriate fear and contributing to disorders, such as post-traumatic stress disorder. However, it is unknown how such poorly formed fear memories are encoded within the brain. We find that restricting learning time results in fear memories that are encoded by fewer hippocampal cells. Moreover, these fewer cells are inappropriately reactivated in both dangerous and safe contexts. These findings suggest that fear memories formed at brief periods overgeneralize because they lack the detail-rich information necessary to support neuronal discrimination.
Effects of 8 years of growth hormone treatment on scoliosis in children with Prader–Willi syndrome
Authors: Grootjen Lionne N , Rutges Joost P H J , Damen Layla , Donze Stephany H , Juriaans Alicia F , Kerkhof Gerthe F , Hokken-Koelega Anita C S 
Published: April 2021

Objective
Scoliosis is frequently seen in children with Prader–Willi syndrome (PWS). There is still concern that growth hormone (GH) treatment might increase the risk of onset or progression of scoliosis. Short-term data suggested no adverse effects of GH on scoliosis, but long-term effects of GH treatment on development of scoliosis in PWS are unknown. This study investigated the effects of 8 years of GH treatment on scoliosis in children with PWS.

Design
Open-label, prospective cohort study in 103 children with PWS receiving GH for 8 years was analyzed. Prevalence and severity of scoliosis were compared to a group of 23 age-matched GH-untreated children with PWS.

Methods
Spine X-rays and DEXA-scans were performed, and Cobb angel was measured by two independent observers.

Results
After 8 years of GH treatment, at median age of 10.8 years, prevalence of scoliosis was 77.7%. No difference in prevalence or severity of scoliosis was found between GH-treated and age-matched untreated children with PWS (P = 0.409 and P = 0.709, respectively). Height SDS and trunkLBM were significantly higher in GH-treated children. Higher bone mineral density of the lumbar spine was found in children without scoliosis after 8 years of GH. Bone mineral apparent density of lumbar spine (BMADLS) SDS was associated with lower Cobb angle (r = −0.270, P = 0.008).

Conclusions
Eight years of GH treatment has no adverse effects on the prevalence and severity of scoliosis in children with PWS until 11 years of age. As BMADLS SDS is inversely associated with Cobb angle, it is pivotal to optimize the BMD status in children with PWS.
Reconstruction of the ligamentous apparatus of the lower pelvis in robot-assisted radical prostatectomy as a stage of the learning curve
Authors: Svetocheva Ya. A. , Slusarenko R. I. , Tsarichenko D. G. , Suhanov R. B. , Bezrukov E. A. , Rapoport L. M. 
Published: April 2021

Objective of the study. To evaluate results of robot-assisted laparoscopic radical prostatectomy in the framework of a surgeon’s learning curve; to evaluate the effect of reconstruction of the ligamentous apparatus of the lower pelvis on early recovery of urine retention.Materials and methods. 246 patients were divided into 3 groups depending on the surgery date. The analyzed data was accumulated prospectively and retrospectively. Preoperative characteristics (TNM stage, PSA, ISUP), intraoperative characteristics (surgical time, blood loss volume, surgery type and character, type of reconstruction of the ligamentous apparatus of the lower pelvis) and postoperative characteristics (duration of bladder drainage with a urethral catheter, level and time of recovery for urine retention and erectile function) were evaluated. Reconstruction of the ligamentous apparatus of the lower pelvis was performed through reconstruction of the Denonvillier fascia (Rocco stitch), as well as through suturing fibers of the bladder neck with periurethral tissues and residual puboprostatic ligaments for stabilization of the urethrovesical complex.Results. All operations were successfully completed without conversions or transfusions. Median surgical time was 160 minutes (p = 0.0001). Median blood loss was 173.3 cm3 (p = 0.0002). Mean prostatic volume was 36 cm3 (29–47.5 cm3), and overall frequency of positive surgical margin was 12.82 %. Overall frequency of urine retention recovery was 51.6 % after 3 months, 63.7 % after 6 months (p >0.05). In the patient group with reconstruction of the ligamentous apparatus of the lower pelvis, frequency of urine retention recovery was higher than in the group without reconstruction: 64.1 % vs 45.3 % (after 3 months, р = 0.041) and 74.7 % vs 62.3 % (after 6 months, р = 0.034). Conclusion. Surgical time, blood loss were significantly decreased with the number of performed operative interventions. Significant improvement of early urine retention recovery was observed in the patient group with reconstruction of the ligamentous apparatus of the lower pelvis.
A case of new coronavirus infection course in a child with severe secondary immunodeficiency associated with hematopoietic stem cell transplantation
Authors: Grymova Natalya , Khuzina Ekaterina A. , Furman Evgeny G. , Nikonova Olga E. 
Published: April 2021

Numerous articles have been published on COVID-19 cases in the child population now. Children become infected and get sick with a new infection much less often and more easily than adults. The supposed relative resistance of children to SARS-CoV-2 can be explained by a number of reasons, one of which is the immaturity of the ACE2 receptor, or by the fact that children have certain features of innate immunity that disappear in ontogenesis. On the contrary, the maturity of the immune system may explain the unfavorable type of triggered immune response associated with the development of acute respiratory distress syndrome in adult patients.
The article describes a case of a new coronavirus infection in a 5-year-old child with a severe secondary immunodeficiency condition associated with hematopoietic stem cell transplantation. By describing this clinical case, we wanted to draw attention to a mild course of COVID-19 infection in a child with a burdened premorbid history in the form of severe secondary immunodeficiency associated with hematopoietic stem cell transplantation in the treatment of acute promyelocytic leukemia.