bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimal Design of Magnetic Torque for a Hydraulic-Magnetic Rotary Hole Cleaning Tool in Horizontal Drilling
Authors: Xiao-feng Sun , Hu Qiao-bo , Qu Jingyu , Li Wei , Mao Ning , Ju Guoshuai 
Published: September 2021

Summary
The cleanliness of wellbore is a key factor in the drilling speed and quality of an oil field, especially in long horizontal sections of horizontal wells. Therefore, a hydraulic-magnetic rotary hole cleaning tool has been designed that does not rely on the rotary action of the drillpipe and could be used with a downhole motor to improve hole cleaning efficiency. However, the influence of magnet shape on the transmission of magnetic torque has remained unclear, such that the magnetic shaft transmission torque needed to be optimized to ensure efficient tool operation. In this study, magnetic field control equations were established in the region of the permanent magnet and air gap, and the magnetic flux distribution and magnetic torque generated between two magnetic axes in each field were calculated. Also, the influence of various magnetic field parameters on magnetic torque conduction of a strip magnet were compared and analyzed and then confirmed by comparison with experimental results. The results showed that the magnetic torque transmitted by strip magnets varied sinusoidally with magnetic axis deviation angles and that the highest torque was generated in the 12-pole model. However, the rate of increase in magnetic torque with magnet thickness was opposite to that of tile magnets, increasing with increasing magnet thickness. Magnetic torque variation with covered area was specific in the 6-pole model, showing a tendency of increasing and then decreasing. When magnet thickness was 12 mm and magnet coverage area in the effective cross section of the tool was 80%, the highest magnetic torque/unit volume of magnet was generated for achieving economic optimization. The results led to conclusions that, by solving the regional magnetic field, the magnetic torque change characteristics during movement of the magnetic drive mechanism of the hydraulic-magnetic rotary hole cleaning tool were simulated successfully and that these results could be used as an optimization analysis method for the magnetic drive mechanism of such tools.
Ligation and supercoiling of plasmids containing single strand interruptions
Authors: Calil Felipe A. , Putnam Christopher D. , Kolodner Richard D. 
Published: September 2021

Abstract
We have developed a simple procedure for determining if a plasmid DNA contains single strand interruptions that can be sealed by DNA ligase and thus contain single strand interruptions with 5'-phosphate and 3'-hydroxyl groups. In this procedure, plasmid DNAs with ligatable nicks were ligated by T4 DNA ligase, supercoiled by E. coli DNA gyrase and analyzed by agarose gel electrophoresis. Our results show that after sealing nicked circular DNA with DNA ligase, supercoiling by DNA gyrase produces a rapidly migrating DNA species that can easily be distinguished from nicked circular DNA by agarose gel electrophoresis.
The Construction and Exploration of the CeRNA Network and Patterns of Tumor-infiltrating Immune Cells in Kidney Renal Clear Cell Carcinoma
Authors: Jia Weiwei , Li Pengjia , Ma Mingxia , Niu Xiaochen , Bai Lina , Hao Haihu , Wang Xiaohui , wang li 
Published: September 2021

Abstract
KIRC is the malignant tumor with the highest incidence and poor prognosis in renal cell carcinoma. We want to explore the possible mechanisms of KIRC and effective prognostic-related biomarkers. The sequencing information of 3 types of RNA (mRNA, lncRNA and miRNA) in 539 cases of KIRC tissues and 72 cases of normal tissues is obtained from the TCGA database. Methods such as univariate Cox regression analysis, lasso regression screening, and multivariate Cox regression analysis were used to construct a prognostic model based on the CeRNA network. There are 3074 mRNAs, 359 lncRNAs and 132 miRNAs differentially expressed that have been identified through differential analysis. A complete mRNA-miRNA-lncRNA (SIX1-hsa-miR-200b-3p-MALAT1) network was obtained based on the CeRNA network. The CIBERSORT algorithm was used to analyze the degree of infiltration of 22 kinds of immune cells from each sample of KIRC. Construction of a prognostic model based on tumor-infiltrating immune cells, 2 immune cells (Mast cells resting, T cells follicular helper) were identified by constructing a prognostic model. There was a negative correlation between lncRNA MALAT1 and Mast cells resting (R= -0.27, P < 0.001); while there was a positive correlation between lncRNA MALAT1 and T cells follicular helper (R = 0.23, P < 0.001).
KSHV Episome Tethering Sites on Host Chromosomes: Regulation of Latency-Lytic Switch by the ChAHP complex
Authors: Kumar Ashish , Lyu Yuanzhi , Yanagihashi Yuichi , Chantarasrivong Chanikarn , Majerciak Vladimir , Salemi Michelle , Wang Kang-Hsin , Chuang Frank , Davis Ryan , Tepper Clifford , Nakano Kazushi , Izumiya Chie , Shimoda Michiko , Nakajima Ken-ichi , Merleev Alexander , Zheng Zhi-Ming , Campbell Mel , Izumiya Yoshihiro 
Published: September 2021

Abstract
Kaposi’s sarcoma-associated herpesvirus (KSHV) establishes a latent infection in the cell nucleus, but where KSHV episomal genomes are tethered and the mechanisms underlying KSHV lytic reactivation are unclear. Here, we study the nuclear microenvironment of KSHV episomes and show that the KSHV latency-lytic replication switch is regulated via viral long non-coding (lnc)RNA-CHD4 (chromodomain helicase DNA binding protein 4) interaction. KSHV episomes localize with a CHD4 complex, ChAHP, at epigenetically active genomic regions and tethers frequently near centromeric regions of host chromosomes. The ChAHP complex also occupies the 5’-region of a highly-inducible lncRNAs and terminal repeats of KSHV genome with latency-associated nuclear antigen (LANA). Viral lncRNA binding competes with CHD4 DNA binding, and KSHV reactivation is accompanied by the detachment of KSHV episomes from host chromosome docking sites We propose a model in which elevated lncRNA expression determines the KSHV latency-lytic decision by regulating LANA/ChAHP DNA binding at inducible viral enhancers.
Empathy And Burnout In Medical Staff: Mediating Role of Job Satisfaction And Job Commitment
Authors: YUE Zongpu , Qin Yang , Li Ying , Wang Jian , Nicholas Stephen , Maitland Elizabeth , Liu Cai 
Published: September 2021

Abstract
Background: Burnout is a growing problem among medical staff worldwide and empathy has been described as an essential competence to attenuate burnout. Previous studies found job satisfaction and job commitment were affected by the empathy and associated with burnout. This study explores the effect and mechanism of empathy on burnout on medical staff and investigates the mediating role of job satisfaction and job commitment in the relationship between empathy and burnout among medical staff.Methods: Based on a self-administered questionnaire, 335 responses from medical staff in Tianjin City, China, yielded data on socio-demographic characteristics, empathy, burnout, job satisfaction and job commitment. Bivariate correlation and structured equation modeling (SEM) analyzed the relationships between empathy, job satisfaction, job commitment and burnout multi-group invariant analysis was used to evaluate whether the model was consistent across different type and level of hospitals and different job and employment type subgroups. Results: A total of 202 (60.3%) medical staff had low level burnout, 115 (34.3%) staff had the moderate level and 18 (5.4%) staff had the high level burnout. The results of the SEM showed that empathy not only had a direct negative effect on burnout (β = -0.401, p < 0.001), but also had an indirect impact through job satisfaction ( β = -0.373, p < 0.001) and job commitment (β = 0.489, p < 0.001). Job commitment was negatively associated burnout ( β = -0.513, p < 0.001) but, unexpectedly, job satisfaction was positively associated with burnout (β = 0.177, p < 0.001). The results also indicated the model was consistent across employment type ( Δχ2 = 5.904, p > 0.05) and hospital type ( Δχ2= 7.748, p > 0.05), but was inconsistent across hospital level (Δχ2 = 42.930, p < 0.05) and job type ( Δχ2= 52.912, p < 0.05).Conclusions: Our results confirm the important role that empathy plays in the prevention of burnout and by managing job satisfaction and increasing the job commitment burnout can be attenuated. We recommend that government should increase pay for medical staff; continue to reform the resourcing of different hospital levels; and that hospital managers implement additional training and strengthen psychological testing and counseling, to reduce medical staff burnout.
Recalibrating the Non-Communicable Diseases Risk Prediction Tools for the Rural Population of Western India
Authors: Gupta Manoj Kumar , Raghav Pankaja , Tanvir Tooba , Gautam Vaishali , Mehto Amit , Choudhary Yachana , Mittal Ankit , Singh Gyanendra , Singh Garima , Baskaran Pritish , VR Rehana , Jabbar Shaima Abdul , Srid S , Goel Akhil Dhanesh , Bhardwaj Pankaj , Saurabh Suman , Srika S , KH Naveen , Prasanna T , Rustagi Neeti , Sharma Prem Prakash 
Published: September 2021

Abstract
Background: The present study was conducted to recalibrate the effectiveness of Indian Diabetes Risk Scores (IDRS) and Community-Based Assessment Checklist (CBAC) by opportunistically screening for Diabetes Mellitus (DM) and Hypertension (HT) among the patients attending health centres, and to estimate the risk of fatal and non-fatal Cardio-Vascular Diseases (CVDs) using WHO/ISH chartMethods: All the people of age ≥30 years attending the health centers were screened for DM and HT. Weight, height, and waist and hip circumferences were measured and BMI and Waist Hip Ratio (WHR) were calculated. Risk categorization of all participants was done using IDRS, CBAC, and WHO/ISH risk prediction charts. Individuals diagnosed with DM or HT were started on treatment. The data was recorded using Epicollect5 and was analyzed using SPSS v.23 and MedCalc v.19.8. ROC curves were plotted for DM and HT with the IDRS, CBAC score and anthropometric parameters. Sensitivity (SN), specificity (SP), Positive Predictive Value (PPV), Negative Predictive Value (NPV), Accuracy and Youden’s index were calculated for different cut-offs of IDRS and CBAC scores.Results: A total of 942 participants were included for the screening, out of them, 6.42 % (95% CI: 4.92-8.20) were diagnosed with DM. Hypertension was detected among 25.7% (95% CI: 22.9-28.5) of the participants. A total of 447 (47.3%) participants were found with IDRS score ≥ 60, and 276 (29.3%) with CBAC score >4. As much as 26.1% were at moderate to higher risk (≥10%) of developing CVDs. Area Under the Curve (AUC) for IDRS in predicting DM was 0.64 (0.58-0.70), with 67.1% SN and 55.2% SP (Youden's Index= 0.22). While the AUC for CBAC was 0.59 (0.53-0.65). For hypertension the both the AUCs were 0.66 (0.62-0.71) and 0.63 (0.59-0.67), respectively.Conclusions: Instead of CBAC, the present study emphasizes the usefulness of IDRS as an excellent tool for screening for both DM and HT. This is the time to expose the hidden part of the NCDs iceberg by having high sensitivity of non-invasive instruments (like IDRS), so, we propose a cut-off value of 50 for the IDRS to screen for diabetes in the rural Indian population.
Expression Patterns of Immune Checkpoints In Breast Cancer Patients
Authors: Qiu Dan , Yan Xianxin , Zhang Guijuan , Xiao Xinqin , Cao Jingyu , Wang Yanqiu , Ma Ruirui , Hong Shouyi , Ma Min 
Published: September 2021

Abstract
Background: immunotherapy with immune checkpoint inhibitors (ICIs) for solid tumors had significantly improved overall survival (OS). Positive response to PD-1/PD-L1 blockades was observed in the treatment of solid tumors. Breast cancer (BC) patients are no exception. However, the efficacy of immunocheckpoint therapy in BC patients remains poor. A particularly important factor is the lack of studies on the expression patterns of immune checkpoints in BC patients. Results: It was found that increased expression of PD-1, PD-L1, STAT1, CTLA-4 was associated with poor OS in BC patients. In addition, co-expression of PD-L1 with PD-1, STAT1or CTLA-4 and co-expression of PD-1 with CTLA-4was related to poor OS. We analyzed associations between the proportionate expression of PD-L1 and PD-1, PD-L1 and STAT1, PD-1 and CTLA-4, PD-1 and LAG3, PD-L1 and CTLA-4 in BC patients, there was significance in correlation in both of the BC patients. The expression of STAT1 in BC patients was compared with that of HC, and it was found that STAT1 was highly expressed in BC patients. Conclusions: our results suggest that transcriptome-based co-expression of STAT1 and PD-L1 is a predictor for poor OS in BC patients, which might provide novel insight into designing combinational targeted therapy for BC.
A Comparison of The Effect of Diurnal Temperature Range And Apparent Temperature On Cardiovascular Disease Among Farmers In Qingyang, Northwest China
Authors: Zhai Guangyu , Qi Jintao , Zhang Xuemei , Zhou Wenjuan , Wang Jiancheng 
Published: September 2021

Abstract
Background: Apparent temperature (AT) and diurnal temperature range (DTR) have been extensively used to evaluate the effects of temperature on cardiovascular disease (CVD). However, few studies have analyzed and compared their effects on CVD in less-developed, rural areas of China.Methods: A case-only analysis was conducted of 43,567 cases of CVD morbidity in Qingyang (Northwest China) in 2011–2017 and the effects of thermal indicators were assessed using distributed lag nonlinear modelling and a Poisson regression model. Data on CVD morbidity originated from the New Rural Cooperative Medical Insurance of Gansu Province and meteorological variables were provided by the Meteorological Science Data Sharing Service.Results: Both AT and DTR had significant nonlinear and delayed impacts on hospital admissions for CVD. DTR had a stronger and more persistent effect on CVD incidence than AT. Women were more affected by high AT and low DTR than were men, while men were more vulnerable to low AT and high DTR. Temperature effects were not significantly different between people above and below 65 years of age.Conclusions: These findings provide local public health authorities with reference data concerning sensitive temperature indices for susceptible populations with a view to improving CVD preventive strategies in rural areas.
Health System Cost of Post-Abortion Care (PAC): A Systematic Review
Authors: Sihaloho Estro Dariatno , Habibie Ibnu , Kamilah Fariza Zahra , Christiani Yodi 
Published: September 2021

Abstract
Background: Despite the increasing trend of Post Abortion Care (PAC) needs and provision, the evidence related to its health system cost is lacking. The study aims to review the health system costs of Post-Abortion Care (PAC) per patient at a national level.Methods: A systematic review of literatures related to PAC cost published in 1994 – October 2020 was performed. Electronic databases such as PubMed, Medline, The Cochrane Library, CINAHL, and PsycINFO were used to search the literature. Following the title and abstract screening, reporting quality was appraised using the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) checklist. PAC costs were extrapolated into, US dollars ($US) and international dollars ($I), both in 2019. Content analysis was also conducted to synthesize the qualitative findings.Results: Twelve studies met the inclusion criteria. All studies reported direct medical cost per patient in accessing PAC, but only three of them included indirect medical cost. All studies reported either average or range of cost. In terms of range, The highest direct cost of PAC with MVA (Medical Vacuum Aspiration) services can be found in Colombia, between $US50.58-212.47, while the lowest is in Malawi ($US15.2-139.19). The highest direct cost of PAC with D&C (Dilatation and Curettage), services was in El Salvador ($US65.22-240.75), while the lowest is in Bangladesh ($US15.71-103.85). Among two studies providing average indirect cost data, Uganda with $US105.04 is the highest average indirect medical cost, while Rwanda with $US51.44 is the lowest on the cost of indirect medical.Conclusions: Our review shows variability in cost of PAC across countries. This study depicts a clearer picture of how costly it is for women to access PAC service, although it is still seemingly underestimated. When a study compared the use of UE method between MVA and D&C, it is confirmed that MVA treatments tend to have lower costs and potentially reduce a significant cost. Therefore, by looking at both clinical and economic perspective, improving and strengthening the quality and accessibility of PAC with MVA is a priority.
Aragonite is calcite’s best friend at the seafloor
Authors: Sulpis Olivier , Agrawal Priyanka , Wolthers Mariette , Munhoven Guy , Walker Matthew , Middelburg Jack 
Published: September 2021

Abstract
In the open ocean, calcium carbonates are mainly found in two mineral forms. Calcite, the least soluble, is widespread at the seafloor, while aragonite, the more soluble, is rarely preserved in marine sediments. Despite its greater solubility, research has shown that aragonite, which could contribute between 10 and 90% to pelagic calcium carbonate production, is able to reach the deep-ocean. If large quantities of aragonite settle and dissolve at the seafloor, this represents a large source of alkalinity that buffers the deep ocean and favours the preservation of less soluble calcite, acting as a deep-sea, carbonate version of galvanization. Here, we investigate the role of aragonite dissolution on the early diagenesis of calcite-rich sediments using a novel 3D, micrometric-scale reactive-transport model combined with 3D, X-ray tomography structures of natural aragonite and calcite shells. Results highlight the important role of diffusive transport in benthic calcium carbonate dissolution, in agreement with recent work. We show that, locally, aragonite fluxes to the seafloor could be sufficient to suppress calcite dissolution in the top layer of the seabed, possibly causing calcite recrystallization. As aragonite producers are particularly vulnerable to ocean acidification, the proposed galvanizing effect of aragonite could be weakened in the future, indirectly boosting calcite dissolution further.
Factors Associated With Circadian Rhythm, Job Burnout, And Perceived Stress Among Nurses In Chinese Tertiary Hospitals: A cross-Sectional Study
Authors: Xie Jianfei , Li Jie , Zhang Chun , Zhou Yi , Luo Xiaofei , Liu Min , Wang Sha , Ding Siqing , Li Lijun , Cheng Andy SK 
Published: September 2021

Abstract
Background: Shift work is a common work pattern for nurses worldwide. Circadian rhythm dysregulation affects the quality of nurses’ work and their physical and psychological health. Eveningness and occupational stress might be associated with burnout syndrome.Methods: A Cross-sectional study design. Between July and September 2020, 23 tertiary hospitals were randomly selected from among 113 tertiary hospitals in Hunan Province for participation in this study. At least 25% of the nurses working in each hospital were targeted. Nurses’ circadian rhythm, job burnout, and perceived stress were evaluated. This paper complies with the STROBE reporting guideline for cross-sectional studies.Results: A total of 28.1% and 17.6% of nurses reported eveningness type and morningness type, respectively. The scores for emotional exhaustion, depersonalization, and perceived stress of eveningness nurses were higher than those of their morningness counterparts. Eveningness nurses also reported a lower sense of personal accomplishment (P<.05). Predictors of MEQ score included age 36-40 years, widowed or divorced, BMI ≥25 kg/m2, history of chronic disease, working for 11-20 years or 21 years and above, emotional exhaustion, personal accomplishment, perceived stress.Conclusions: A large proportion of nurses experience circadian rhythm dysregulation. It is necessary to understand the predictors and influencing factors of circadian rhythm dysregulation in nurses to relieve their job burnout and reduce perceived stress.
Inventory of leaf and flower odorants in plants associated with the life cycle of Japanese Papilio butterflies
Authors: Inoue Takashi A. , Otani Hitomi , Niihara Kinuko , Fukuda Tatsuya 
Published: September 2021

Abstract
The odorants of eight Japanese mainland native species (Citrus x deliciosa, Zanthoxylum ailanthoides, Z. schinifolium, Z. piperitum, Phellodendron amurense, Orixa japonica, Skimmia japonica, and Boenninghausenia albiflora), one tropical species (Euodia meliifolia), and one invasive species (Ruta graveolens) of the Rutaceae family and three Japanese mainland native species (Angelica keiske, Heracleum lanatum, Anthriscus sylvestris, and one invasive species (Foeniculum vulgare) of the Apiaceae family were analyzed using gas chromatography–mass spectrometry with dynamic–headspace and thermal–desorption methods. These plants are hostplants to Japanese Papilio butterflies. Herein, these 14 plants were classified into six major groups based on the odorant volatiles, which did not correspond to the current phylogenetic classification. Similarly, floral odorant analysis of the six plant species (Clerodendrum trichotomum, Cayratia japonica, Robinia pseudoacacia, Lonicera japonica, C. deliciosa, Z. ailanthoides) visited by Papilio butterflies for nectaring, revealed the presence of linalool in all the flowers. Floral volatiles in C. deliciosa and Z. ailanthoides exhibited moderate resemblance to their respective leaf volatiles. Interestingly, our results in C. trichotomum was not in complete agreement with previous reports, emphasizing the need for newer methods of extraction and analysis.