bluuebunny/crossref_metadata_2025_split huggingface dataset:
Métodos de análise da composição química e valor nutricional de alimentos para ruminantes
Authors: Carvalho Chrislanne Barreira de Macêdo , Macambira Gabriel Miranda , Santos Ana Carolina Ferreira dos , Oliveira Helia Sharlane de Holanda , Silva Dayane Albuquerque da , Ribeiro Apolônio Gomes , Silva Gabriela Duarte , Pessoa Diana Valadares , Maciel Marilene dos Santos , Marinho Jéssica Berly Moreira , Medeiros Aurielle Silva , Soares Géssica Solanna Calado , Sales Tafnes Bernardo , Silva Pedro Henrique Ferreira da , Silva Gleyce Kelly de França 
Published: August 2021

A análise dos alimentos constitui um dos principais fatores observados na nutrição animal. A forma mais eficiente de identificação do teor de nutrientes dos alimentos, é através da composição química e valor nutritivo. Na quantificação analítica do valor nutritivo dos alimentos, os principais parâmetros utilizados são: matéria seca (MS), métodos de secagem em estufa, forno de micro-ondas e destilação com tolueno (silagens); matéria mineral (MM), método da incineração em altas temperaturas em mufla; proteína bruta (PB) ou nitrogênio total (N), método Dumas, Linder, Nessler, e Kjeldahl (padrão); fibra detergente neutro (FDN) e fibra detergente ácido (FDA), método Van Soest, com adaptações de equipamentos; lignina, método hidrólise ácida (padrão), com permanganato de potássio, Klason, e lignina solúvel em brometo de acetila; e digestibilidade, métodos in vivo, in situ, in vitro, e marcadores de digestibilidade. No entanto, estes podem ser onerosos, caros e demandar bastante tempo. Como método alternativo e indireto, tem-se a espectrometria de reflectância no infravermelho próximo (NIRS), que possui vantagens com custos, rapidez, usa um pequeno número de amostras e amostragem não destrutiva, é multiparamétrico, e não poluente. Considerando as diversas variáveis que podem ser utilizadas para determinação do valor nutritivo dos alimentos para ruminantes e a gama dos métodos analíticos disponíveis na literatura, cabe ao observador adotar aquele que melhor convém ao objetivo proposto, levando em consideração o tipo de alimento, custo, disponibilidade de reagentes, materiais, equipamentos, e animais à disposição. A metodologia adotada foi um estudo descritivo, resultando em uma revisão bibliográfica embasada em artigos científicos mundiais.
Educação Profissional Técnica de Nível Médio em Saúde/Enfermagem no Sistema Único de Saúde
Authors: Corrêa Adriana Katia , Souza Maria Conceição Bernardo de Mello e , Clapis Maria José 
Published: August 2021

Objetivo: apresentar e analisar um panorama da educação profissional técnica de nível médio na área da saúde, considerando cursos ofertados, matrículas e concluintes, no estado de São Paulo, de 2010 a 2015, com ênfase na enfermagem. Metodologia: pesquisa longitudinal, retrospectiva, caracterizada também como estudo de caso, fundamentado na perspectiva histórico-dialética, incluindo 24 cursos do Catálogo Nacional dos Cursos Técnicos (terceira versão), com base em dados do Instituto Nacional de Estudos e Pesquisas Educacionais Anísio Teixeira (INEP) - MEC, de 2010 a 2015. Resultados: foram ofertados 23 desses cursos, com aumento de oferta de 2010 a 2015. A enfermagem é o curso com mais oferta, matrículas e concluintes. A presença do setor privado e da modalidade subsequente é predominante. Conclusões: Essa conformação se fortalece no cenário político neoliberal, sendo contraditória à necessidade de formação que apoie o fortalecimento do SUS, a partir das necessidades sociais, bem como que promova emancipação do trabalhador.
Curso online de orçamento público para os servidores técnico-administrativos de uma instituição pública de ensino federal
Authors: Yukimura Juliana Novo Paccola , Otani Nilo , Pasqualli Roberta 
Published: August 2021

O texto tem como objetivo apresentar o desenvolvimento do protótipo de um curso no ambiente virtual de aprendizagem Moodle, destinado aos Servidores Técnico-Administrativos em Educação (TAEs) que atuam na Universidade Federal de Santa Catarina (UFSC), sobre orçamento público, com enfoque nos aspectos do orçamento da Universidade. Para atingir este objetivo, foi realizada pesquisa documental, em que foram analisados dados orçamentários da instituição, obtidos por meio de relatórios e leis orçamentárias. Dados empíricos também foram coletados com os TAEs, integrantes do quadro da UFSC por meio de questionário aplicado com o apoio da ferramenta de formulários do Google Drive. O questionário teve como objetivo subsidiar a criação do curso online de orçamento público para os TAEs e, por meio dele, buscou-se entender o grau de interesse dos servidores técnicos administrativos em tal curso e o que, na percepção desses trabalhadores, é importante ser abordado. Também foi realizado um grupo focal com os servidores que atuam na Superintendência de Orçamento e no Departamento de Contabilidade e Finanças da UFSC. Buscou-se compreender quais são as expectativas quanto ao que seria abordado no curso online e o que esses servidores percebiam como fundamental que fosse ensinado a fim de qualificar o trabalho dos TAEs. Como repercussões, destaca-se que a metodologia PACO, criada por pesquisadores da Universidade Federal de São Carlos serviu de guia para a criação do protótipo do curso. O curso foi aplicado e avaliado de forma positiva com indicação de qualificação do trabalho com orçamento público na UFSC.
Modelo para mejorar el planeamiento presupuestal y la interoperabilidad de la Escuela de Posgrado de la Universidad Nacional de Tumbes
Authors: León Tinoco Gaby Doraliza , Vértiz Querévalu Pedro José , Chávez Roncal Luis Alberto 
Published: August 2021

La investigación se realizó con el objetivo de diseñar un modelo conceptual teórico, que permita mejorar la planeación presupuestal y la interoperabilidad intra-administrativa de la Escuela de Posgrado de la Universidad Nacional de Tumbes. El estudio fue de enfoque cuantitativo, de nivel descriptivo y correlacional; diseño no experimental de corte transversal; la muestra estuvo conformada por 294 colaboradores, entre directivos, administrativos y estudiantes de los programas de maestrías y doctorados de la Escuela de Posgrado de la Universidad Nacional de Tumbes. Se utilizó como técnica la encuesta; los instrumentos se elaboraron en formularios de Google, fueron sometidos a validación y confiabilidad, obteniéndose el Alfa de Cronbach de 0.938 para el instrumento aplicado al personal administrativo y de 0.909 para el instructivo aplicado a los estudiantes. Los datos se procesaron en el Statistical Package for the Social Sciences – SPSS 26, lo que permitió obtener información y diseñar el modelo conceptual teórico, para mejorar la planeación presupuestal e interoperabilidad intra- administrativa de la Escuela de Posgrado Universidad Nacional de Tumbes. Concluyendo que la implementación teórica a priori del modelo, permitirá que la Escuela de Posgrado de la Universidad Nacional de Tumbes, incluya en su Plan Operativo Institucional las actividades que requiere la interoperabilidad intra-administrativa, logrando con su puesta en marcha modernizar a la institución.
EQCM Analysis of the Insertion Phenomena in a n-Doped Poly-Alkyl-Terthiophene With Regioregular Pattern of Substitution
Authors: Dini Danilo , Salatelli Elisabetta , Decker Franco 
Published: August 2021

In the present work, we have undertaken the study of the n-doping process in poly-3,3″-didodecyl-2,2′:5′,2″-terthiophene (poly-33″-DDTT) employing the electrochemical quartz crystal microbalance (EQCM). The present study aims at understanding how cathodic charge in n-doped poly-33″-DDTT is compensated. For this purpose, the in situ analysis of the variations of the polymeric mass has been considered. Poly-33″-DDTT was obtained as a thin coating onto a metallic substrate via the anodic coupling of the corresponding monomer 3,3″-didodecyl-2,2′:5′,2″-terthiophene (33″-DDTT). When subjected to electrochemical n-doping in the polarization interval -2.5 ≤ Eappl ≤ 0 V vs. Ag/Ag+, the films of poly-33″-DDTT varied their mass according to a mechanism of cations insertion during n-doping and cations extraction during polymer neutralization. In fact, the electrochemical doping of polythiophenes requires the accompanying exchange of charged species to maintain the electroneutrality within the structure of the polymer in all states of polarization. At the end of a full electrochemical cycle (consisting of the n-doping and the successive neutralization of poly-33″-DDTT), the polymer retains a fraction of the mass acquired during n-doping, thus manifesting the phenomena of mass trapping. The combined analysis of electrochemical and microgravimetric data suggests that poly-33″-DDTT in the n-doped state undergoes (or electrocatalyzes) uncontrolled electrochemical reactions that are not accompanied by mass variations.
Determinants and Prediction Equations of Six-Minute Walk Test Distance Immediately After Cardiac Surgery
Authors: Radi Basuni , Ambari Ade Meidian , Dwiputra Bambang , Intan Ryan Enast , Triangto Kevin , Santoso Anwar , Setianto Budhi 
Published: August 2021

Background: To date, there is no reference for a 6-min walk test distance (6-MWD) immediately after cardiac surgery. Therefore, this study aimed to identify the determinants and to generate equations for prediction reference for 6-MWD in patients immediately after cardiac surgery.Methods: This is a cross-sectional study of the 6-min walk test (6-MWT) prior to participation in the cardiac rehabilitation (CR) program of patients after coronary artery bypass surgery (CABG) or valve surgery. The 6-MWT were carried out in a gymnasium prior to the CR program immediately after the cardiac surgery. Available demographic and clinical data of patients were analyzed to identify the clinical determinants of 6-MWD.Results: This study obtained and analyzed the data of 1,509 patients after CABG and 632 patients after valve surgery. The 6-MWD of all patients was 321.5 ± 73.2 m (60–577). The distance was longer in the valve surgery group than that of patients in the CABG group (327.75 ± 70.5 vs. 313.59 ± 75.8 m, p &lt; 0.001). The determinants which significantly influence the 6-MWD in the CABG group were age, gender, diabetes, atrial fibrillation, and body height, whereas in the valve surgery group these were age, gender, and atrial fibrillation. The multivariable regression models generated two formulas using the identified clinical determinants for patients after CABG: 6-MWD (meter) = 212.57 + 30.47 (if male gender) − 1.62 (age in year) + 1.09 (body height in cm) − 12.68 (if with diabetes) − 28.36 (if with atrial fibrillation), and for patients after valve surgery with the formula: 6-MWD (meter) = 371.05 + 37.98 (if male gender) − 1.36 (age in years) − 10.61 (if atrial with fibrillation).Conclusion: This study identified several determinants for the 6-MWD and successively generated two reference equations for predicting 6-MWD in patients after CABG and valve surgery.
First-in-Human Evaluation of Positron Emission Tomography/Computed Tomography With [18F]FB(ePEG12)12-Exendin-4: A Phase 1 Clinical Study Targeting GLP-1 Receptor Expression Cells in Pancreas
Authors: Fujimoto Hiroyuki , Fujita Naotaka , Hamamatsu Keita , Murakami Takaaki , Nakamoto Yuji , Saga Tsuneo , Ishimori Takayoshi , Shimizu Yoichi , Watanabe Hiroyuki , Sano Kohei , Harada Norio , Nakamura Hiroshi , Toyoda Kentaro , Kimura Hiroyuki , Nakagawa Shunsaku , Hirai Mitsuharu , Murakami Atsushi , Ono Masahiro , Togashi Kaori , Saji Hideo , Inagaki Nobuya 
Published: August 2021

Pancreatic β-cell mass (BCM) has a central importance in the pathophysiology of diabetes mellitus. Recently, pancreatic β-cell-specific imaging, especially positron emission tomography (PET) with exendin-based probes, has emerged for non-invasive evaluation of BCM. We developed a novel exendin-based probe labeled with fluorine-18, [18F]FB(ePEG12)12-exendin-4 (18F-Ex4) for PET imaging. We subsequently conducted a first-in-human phase 1 study of 18F-Ex4 PET/computed tomography (CT) and investigated the safety and utility for visualizing the pancreas. Six healthy male subjects were enrolled in this study. A low dose (37.0 MBq) of 18F-Ex4 PET/CT was administered (first cohort: n = 2), and subsequently a higher dose (74.0 MBq) was administered (second cohort: n = 4). In the first and second cohorts, 38.6 ± 4.8 and 71.1 ± 4.8 MBq of 18F-Ex4 were administered, respectively. No serious adverse events were observed in both groups. Only one participant in the first cohort showed transient hypoglycemia during the PET scans. 18F-Ex4 PET/CT successfully visualized the pancreas in all participants. The mean standardized uptake value of the pancreas was found to be higher than that in the surrounding organs, except for the bladder and kidney, during the observation. Dosimetry analyses revealed the effective systemic doses of 18F-Ex4 as 0.0164 ± 0.0019 mSv/MBq (first cohort) and 0.0173 ± 0.0020 mSv/MBq (second cohort). 18F-Ex4 PET/CT demonstrated the safety and utility for non-invasive visualization of the pancreas in healthy male subjects. 18F-Ex4 is promising for clinical PET imaging targeting pancreatic β cells.
Serum Anti-PDLIM1 Autoantibody as Diagnostic Marker in Ovarian Cancer
Authors: Qiu Cuipeng , Duan Yaru , Wang Bofei , Shi Jianxiang , Wang Peng , Ye Hua , Dai Liping , Zhang Jianying , Wang Xiao 
Published: August 2021

BackgroundSerum autoantibodies (AAbs) against tumor-associated antigens (TAAs) could be useful biomarkers for cancer detection. This study aims to evaluate the diagnostic value of autoantibody against PDLIM1 for improving the detection of ovarian cancer (OC).MethodsImmunohistochemistry (IHC) test in tissue array containing 280 OC tissues, 20 adjacent tissues, and 8 normal ovarian tissues was performed to analyze the expression of PDLIM1 in tissues. Enzyme-linked immunosorbent assay (ELISA) was employed to measure the autoantibody to PDLIM1 in 545 sera samples from 182 patients with OC, 181 patients with ovarian benign diseases, and 182 healthy controls.ResultsThe results of IHC indicated that 84.3% (236/280) OC tissues were positively stained with PDLIM1, while no positive staining was found in adjacent or normal ovarian tissues. The frequency of anti-PDLIM1 autoantibody was significantly higher in OC patients than that in healthy and ovarian benign controls in both training (n=122) and validation (n=423) sets. The area under the curves (AUCs) of anti-PDLIM1 autoantibody for discriminating OC from healthy controls were 0.765 in training set and 0.740 in validation set, and the AUC of anti-PDLIM1 autoantibody for discriminating OC from ovarian benign controls was 0.757 in validation set. Overall, it was able to distinguish 35.7% of OC, 40.6% of patients with early-stage, and 39.5% of patients with late-stage. When combined with CA125, the AUC increased to 0.846, and 79.2% of OC were detected, which is statistically higher than CA125 (61.7%) or anti-PDLIM1(35.7%) alone (p&lt;0.001). Also, anti-PDLIM1 autoantibody could identify 15% (18/120) of patients that were negative with CA125 (CA125 &lt;35 U/ml).ConclusionsThe anti-PDLIM1 autoantibody response in OC patients was positively correlated with PDLIM1 high expression in OC tissues, suggesting that the autoantibody against PDLIM1 might have the potential to be a novel serological biomarker of OC, serving as a complementary measure of CA125, which could improve the power of OC detection.
Dependence of Biofuel Ignition Chemistry on OH-Initiated Branching Fractions
Authors: Hartness Samuel W. , Rotavera Brandon 
Published: August 2021

Abstraction of hydrogen by ȮH is the dominant initiation step in low-temperature oxidation of biofuels. Theoretical chemical kinetics calculations for such reactions provide a direct means of quantifying rates of abstraction, which are critical to modeling biofuel combustion. However, in several cases and despite agreement on total rate coefficients, branching fractions (i.e. the distribution of initial radicals) can vary depending on the level of theory, which leads to variations in ignition delay time predictions. To examine the connection between branching fractions and ignition delay time predictions, simulations were conducted for 1-butanol, cyclopentanone, and methyl propanoate at 10 atm and from 500–1000 K. For each case, the simulations utilized recent combustion mechanisms to produce an initial set of ignition delay time trends. H-abstraction rates were then replaced using rates from the literature to examine the effects of ȮH-initiated branching fractions on ignition chemistry. Branching fractions were found to significantly influence ignition chemistry, specifically in the case of 1-butanol, even when total rate coefficients were relatively consistent. From comparison of site-specific rates in the literature, branching fractions for initiation of 1-butanol and methyl propanoate are not consistent, which resulted in ignition delay times differing by factors of up to 6.3 and 1.2 respectively. Conversely, in the case of cyclopentanone, for which both the total and the site-specific rate coefficients agree, ignition delay times were unaffected. From the observed dependence of ignition delay times on ȮH-initiated branching fractions, an intermediate step in the development of combustion mechanisms is necessary to validate site-specific rate coefficients and ensure accurate model predictions. Speciation measurements are one example that can provide a critical link to radical-specific, fundamental chemical pathways and determine accurate branching fractions.
Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats
Authors: Abu Almaaty Ali H. , Elmasry Reham A. , Farrag Mayada S. , Althobaiti Fayez , Aldhahrani Adil , Fayad Eman , Hussain Mona A. 
Published: August 2021

Background: Acute kidney injury (AKI), also known as acute renal failure (ARF), has received considerable critical attention in recent years. Gentamicin (GM) is an antibiotic whose prolonged use results in AKI with a high mortality rate.Methods: Fifty adult female albino rats weighing 150–200 g were used. The animals were divided into five groups: the first group was the normal healthy control one, the second group received only 1 × 106 HUCB mononuclear cells (MNCs)/rat by intravenous (iv) injection, the third diseased group was given GM 100 mg/kg for 10 consecutive days by intraperitoneal injections, the fourth preventive group received 1 × 106 HUCB MNCs/rat by iv injection 24 h before gentamicin treatment, and the fifth treated group received 1 × 106 HUCB MNCs/rat by iv injection 24 h after gentamicin treatment. After 1 week of treatment, blood samples were collected, and kidneys were removed for histopathological examination.Results: Rats treated with HUCB MNCs in the treated group had a significant decrease in renal damage, low levels of biomarkers for nephrotoxicities such as serum creatinine and blood urea nitrogen, and low chromosomal aberrations compared to the diseased third group. The gene expression of KIM-1 and NGAL was decreased in response to HUCB treatment.Conclusions: HUCB MNCs have a curative effect against AKI and gentamicin-induced genotoxicity owing to their regenerative property.
Frailty Predicts Poor Prognosis of Patients After Percutaneous Coronary Intervention: A Meta-Analysis of Cohort Studies
Authors: Wang Peng , Zhang Shutang , Zhang Ke , Tian Jie 
Published: August 2021

Background: Frailty has been related to a higher risk of cardiovascular events, while the association between frailty and outcomes for patients with coronary artery disease (CAD) after percutaneous coronary intervention (PCI) remains unclear. We performed a meta-analysis of cohort studies to evaluate the above association.Methods: Cohort studies aiming to determine the potential independent association between frailty and clinical outcomes after PCI were identified by search of PubMed, Embase, and Web of Science databases from inception to February 22, 2021. A random-effects model that incorporates the possible heterogeneity among the included studies was used to combine the results.Results: Ten cohort studies with 7,449,001 patients were included. Pooled results showed that frailty was independently associated with higher incidence of all-cause mortality [adjusted risk ratio (RR) = 2.94, 95% confidence intervals (CI): 1.90–4.56, I2 = 56%, P &lt; 0.001] and major adverse cardiovascular events [(MACEs), adjusted RR = 2.11, 95% CI: 1.32–3.66, I2 = 0%, P = 0.002]. Sensitivity analyses limited to studies including elderly patients showed consistent results (mortality: RR = 2.27, 95% CI: 1.51–3.41, I2 = 23%, P &lt; 0.001; MACEs: RR = 2.44, 95% CI: 1.44–4.31, I2 = 0%, P = 0.001). Subgroup analyses showed that characteristics of study design, follow-up duration, or type of PCI did not seem to significantly affect the associations (P-values for subgroup analyses all &gt;0.05).Conclusions: Frailty may be an independent risk factor of poor prognosis for patients with CAD after PCI.
Differential Genomic Profile in TERT, DSP, and FAM13A Between COPD Patients With Emphysema, IPF, and CPFE Syndrome
Authors: Guzmán-Vargas Javier , Ambrocio-Ortiz Enrique , Pérez-Rubio Gloria , Ponce-Gallegos Marco Antonio , Hernández-Zenteno Rafael de Jesus , Mejía Mayra , Ramírez-Venegas Alejandra , Buendia-Roldan Ivette , Falfán-Valencia Ramcés 
Published: August 2021

Background: Genetic association studies have identified single nucleotide polymorphisms (SNPs) associated with lasting lung diseases such as Chronic Obstructive Pulmonary Disease (COPD) and Idiopathic Pulmonary Fibrosis (IPF), as well as the simultaneous presentation, known as Combined Pulmonary Fibrosis and Emphysema (CPFE) Syndrome. It is unknown if these diseases share genetic variants previously described in an independent way. This study aims to identify common or differential variants between COPD, IPF, and CPFE.Materials and methods: The association analysis was carried out through a case-control design in a Mexican mestizo population (n = 828); three patients' groups were included: COPD smokers (COPD-S, n = 178), IPF patients (n = 93), and CPFE patients (n = 16). Also, two comparison groups were analyzed: smokers without COPD (SWOC, n = 367) and healthy subjects belonging to the Mexican Pulmonary Aging Cohort (PAC, n = 174). Five SNPs in four genes previously associated to interstitial and obstructive diseases were selected: rs2609255 (FAM13A), rs2736100 (TERT), rs2076295 (DSP) rs5743890, and rs111521887 (TOLLIP). Genotyping was performed by qPCR using predesigned Taqman probes.Results: In comparing IPF vs. PAC, significant differences were found in the frequency of the rs260955 G allele associated with the IPF risk (OR = 1.68, p = 0.01). Also, the genotypes, GG of rs260955 (OR = 2.86, p = 0.01) and TT of rs2076295 (OR = 1.79, p = 0.03) were associated with an increased risk of IPF; after adjusting by covariables, only the rs260955 G allele remain significant (p = 0.01). For the CPFE vs. PAC comparison, an increased CPFE risk was identified since there is a difference in the rs2736100 C allele (OR = 4.02, p &lt; 0.01; adjusted p &lt; 0.01). For COPD-S, the rs2609255 TG genotype was associated with increased COPD risk after adjusting by covariables.Conclusion: The rs2736100 C allele is associated with decreased IPF risk and confers an increased risk for CPFE. Also, the rs2076295 TT genotype is associated with increased IPF risk, while the GG genotype is associated with CFPE susceptibility. The rs2609255 G allele and GG genotype are associated with IPF susceptibility, while the TG genotype is present in patients with emphysema.