bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Identification of Multidrug Resistant Microorganisms Including Bergyella Zoohelcum Acquired from the Skin/Prosthetic Interface of Amputees and Their Susceptibility to Medihoney™ and Garlic Extract (Allicin)
Authors: Harsent Ruby , Smith Paul , Blaxland James Arthur , Rushmere Neil 
Published: November 2021

Abstract
BackgroundUsers of prosthetic devices face the accumulation of potentially drug-resistant pathogenic bacteria on the skin/prosthesis interface. In this study, we took surface swabs of the skin/prosthesis interface of eleven disabled athletes to identify microorganisms present. In addition to determining their antimicrobial resistance profile, we assessed their sensitivity to Manuka honey and Garlic extract (allicin) MethodsEleven volunteers were directed to swab the skin at the skin/prosthesis interface. After initial isolation of microorganisms we employed the following general microbiological methods; Gram stain, Catalase test, Oxidase test, lactose fermenting capability, haemolytic capability, Staphaurex, mannitol fermenting capability, Streptex; API Staph, 20E, Candida, and BBL crystal identification system tests. Once identified, isolates were analysed for their sensitivity to penicillin, erythromycin. ampicillin, vancomycin, ceftazidime, ciprofloxacin, gentamicin and colistin-sulphate. Isolates were also analysed for their sensitivity to allicin (Garlic Extract (GE)) and Manuka honey (Medihoney™) (MH). ResultsEleven isolates were identified, Bacillus cereus, Staphylococcus haemolyticus, Staphylococcus aureus, Micrococcus luteus, Pseudomonas oryzihabitans, Micrococcus spp., Bacillus subtilis, Group D Streptococcus, Pantoea spp., Enterobacter cloacae and Bergyella zoohelcum. All Gram-positive organisms were resistant to 1.5 units of penicillin and 10 μg of ampicillin, and two Gram-negatives Pseudomonas oryzihabitans and Bergyella zoohelcum were resistant to 10 μg ceftazidime, whilst Bergyella zoohelcum, was also resistant to 10 μg of gentamicin. In comparison, all organisms were sensitive to Manuka honey and nine sensitive to Allicin. ConclusionsThis study highlights the prevalence of uncommon drug resistant microorganisms on the skin within a vulnerable population, highlighting the potential for MH or GE intervention.
A Novel Signature to Predict Thyroid Cancer Prognosis and Immune Landscape Using Immune-Related LncRNA Pairs
Authors: SONG BO , Tian Lijun , Zhang Fan , Lin Zheyu , Gong Boshen , Liu Tingting , Teng Weiping 
Published: November 2021

Abstract
Background: Thyroid cancer (TC) is the most common endocrine malignancy worldwide. The incidence of TC is high and increasing worldwide due to continuous improvements in diagnostic technology. TC is still often overtreated due to a lack of reliable diagnostic biomarkers. Therefore, determining accurate prognostic predictions to stratify TC patients is important.Methods: Raw data were downloaded from the TCGA database, and pairwise comparisons were applied to identify differentially expressed immune-related lncRNA (DEirlncRNA) pairs. Then, we used univariate Cox regression analysis and a modified Lasso algorithm on these pairs to construct a risk assessment model for TC. Next, TC patients were assigned to high- and low-risk groups based on the optimal cutoff score of the model for the 1-year ROC curve. We evaluated the signature in terms of prognostic independence, predictive value, immune cell infiltration, ICI-related molecules and small-molecule inhibitor efficacy. Results: We identified 30 DEirlncRNA pairs through Lasso regression, and 14 pairs served as the novel predictive signature. The high-risk group had a significantly poorer prognosis than the low-risk group. Cox regression analysis revealed that this immune-related signature can predict prognosis independently and reliably for TC. With the CIBERSORT algorithm, we found an association between the signature and immune cell infiltration. Additionally, several immune checkpoint inhibitor (ICI)-related molecules, such as PD-1 and PD-L1, showed a negative correlation with the high-risk group. We further found that some commonly used small-molecule inhibitors, such as sunitinib, were related to this new signature. Conclusions: We constructed a prognostic immune-related lncRNA signature that can predict TC patient survival without considering the technical bias of different platforms, and this signature also sheds light on TC overall prognosis and novel clinical treatments, such as ICB therapy and small molecular inhibitors.
Egg production performance of three strains of laying hens kept in floor pens
Authors: Yigzaw Mebratu , Demeke Solomon , Hassen Wasihun 
Published: November 2021

Abstract
Genetic limitation is one of the major bottle necks for the development of the poultry production and the expansion of the commercial poultry production is limited by inadequate supply of high performing chicken breeds locally in Ethiopia. Research system is currently attempting to alleviate this problem by introducing improved poultry breeds that can adapt and perform in Ethiopia. This experiment was carried out to study the on station egg production performance of Lohman Brown, Novo Brown and Dominant Sussex breeds under the objective condition of Jimma. One hundred fifty of each of Dominant Sussex, Lohman Brown and Novo Brown breed of layers obtained from Debre Zeit Research Station was placed at Jucavm layer house on standard commercial layers’ ration in completely randomized design with three replicates. Finally, all the data collected on performance of the experimental breeds of chickens were subjected to statistical analysis. There was no significant difference (P<0.05) between the three breeds tested in age at the first egg, even if Lohman Brown tended to lay comparatively earlier (137 days). The hen day egg production rate and egg weight of Dominant Sussex was better than other two breeds. In summary, the results of this experiment indicated that the three breed of chicken have well performed (except the adaptive potential of Dominant Sussex chickens) under Jimma condition in most of the economically important production traits studied. However, comparative evaluation of the egg quality and reproductive performance of the three breeds seems to be the future direction of research under objective condition of Jimma.
Diversity Analysis of Soil Microbial Population Abundance Before and After Planting JunCao "Oasis No. 1" in Saline-Alkali Soil
Authors: Xiao ZhiQi , Lin ZhanXi 
Published: November 2021

Abstract
In order to explore the difference of soil microbial population structure and abundance before and after planting JunCao"Oasis No. 1" in saline-alkali soil, verify the improvement effect of JunCao"Oasis No. 1" on microbial population structure and abundance in saline-alkali soil. Samples were collected from the blank saline area with and without JunCao"Oasis NO.1" and no plant growth on the surface, respectively, as Experimental group soil samples (S.Y.1-S.Y.8) and Blank group soil samples (K.B.1-K.B.8).16sDNA high-throughput sequencing technology was used for sequencing analysis respectively, and the diversity of microbial population abundance between them was compared and analyzed.The results showed that the diversity of microbial population abundance in the experimental group was significantly higher than that in the blank group, and the diversity of microbial population abundance in the experimental group was significantly different from that in the blank group, indicating that the composition of microbial population in the experimental group was significantly different from that in the blank group. In the OTU cluster analysis, the number of OTU clusters in the Experimental group soil samples (S.Y.1-S.Y.8) was significantly higher than that in the Blank group soil samples (K.B.1-K.B.8). In the sample complexity analysis of α-diversity analysis, the richness and diversity of microbial population in soil samples of Experimental group (S.Y.1-S.Y.8) were significantly higher than that in soil samples of Blank group (K.B.1-K.B.8), which was clearly reflected in the Species accumulation boxplot and Graph of species diversity. In the β-diversity analysis, PcoA, PCA and NMDS analysis methods were used to analyze the difference of microbial population diversity between Experimental soil samples (S.Y.1-S.Y.8) and Blank soil samples (K.B.1-K.B.8). The results showed that the diversity of microbial population in Experimental soil sample (S.Y.1-S.Y.8) was significantly different from that in Blank soil sample (K.B.1-K.B.8). In this paper, 16sDNA high-throughput sequencing technology was used to analyze the diversity of microbial population abundance between Blank soil samples and Experimental soil samples, and it was proved that JunCao"Oasis No. 1" had good saline-alkali soil improvement characteristics. It can effectively increase the abundance of microbial population in saline-alkali soil, so as to restore the microbial population ecosystem in saline-alkali soil, which has important application value in soil saline-alkali control.
Fluorine Speciation in Loess in The Shaanxi Loess Plateau, Related Quality Assessment And Exposure Risks Implication
Authors: Deng Lin , Wang Jiangxia , Xu Bin , Yang Xiaoting , Hu Anyan 
Published: November 2021

Abstract
In this study, distributing characteristics of five fluorine (F) species in loess in the Shaanxi Loess Plateau (SLP) were determined including unextractable residual F (Fre), extractable water-soluble F (Fws), exchangeable F (Fex), F bound to Fe-Mn oxides (Ffm), and F bound to organics (For). The results show that unextractable Fre represents most percentage of Ftf in loess. Of the four extractable F species, the content of Fws is higher than the other three extractable F species in most loess samples. The loess in the southeast SLP has the highest Fws content while the loess in the southwest has the highest Ftf content. And the loess in the north SLP contains the lowest overall concentrations of Ftf and Fws. Similar to Fws, the respective content of Fex, Ffm, and For in the loess is also very low. However, the total of them and Fws cannot be negligible for F mobility in loess. Overall, the loess in the SLP can be assessed as inferior quality in terms of F as a result of high Fws content in most samples. Based on the F speciation in the loess, F exposure to grazing animals and humans, in fact to children, was assessed, indicating that F in the loess in the southwest SLP might pose a much greater risk to grazing animals than in other part of SLP as the loess contains more Ftf. However, there is no concern for the children’s exposure to loess F via ingestion in the study area in the present condition. As for the loess F risk to groundwater, it can be concluded that not only Fws but also the other extractable F including Fex, Ffm and For in loess may be responsible for the F enrichment in local groundwater in the area with the aid of the characteristics of F speciation in loess and distribution of local high-fluoride groundwater.
The Role of Telemedicine for Symptoms Management in Oral Medicine: Retrospective Study
Authors: Alsafwani Zahra , Shiboski Caroline , Villa Alessandro 
Published: November 2021

Abstract
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had devastating effect on access to care in many specialties and dental services including oral medicine. Following the shelter‐in‐place orders in March 2020, we implemented Tele(oral)medicine practices for the diagnosis and management of some oral medicine conditionsObjectives: To assess the effectiveness of telemedicine visits with respect to pain control among patients affected by oral diseases. Methods: A retrospective chart review for all the new patients seen at their first visit via telemedicine between April 2020 and December 2020. The patient-reported pain score was recorded at each visit using a 0-10 scale. Differences in oral pain from the first fist to the follow-up visit of the patients were evaluated using the Wilcoxon signed-rank test. Results: A total of 137 new patients were included with a median age of 56 years. If seen in person, patients would have travelled a median distance of 65 miles. The most common oral conditions seen were reactive/inflammatory lesions. There was a 3-point median pain reduction from the first video visit to the first follow-up (p<0.05) and a self-reported 65% median improvement of oral symptoms. Conclusion: Tele(oral)medicine was an effective method for symptoms management of oral medicine conditions.
Carrington Longitudinal and Solar Cycle Distribution of the Super Active Regions From 1976 to 2018
Authors: Zhao MIng-Xian , Le Guiming , Liu Yonghua , Mao Tian 
Published: November 2021

Abstract
We studied the Carrington longitudinal and solar cycle distribution of the super active regions (SARs) from 1976to 2018. There were 51 SARs during this period. We divided the SARs into SARs1 and SARs2. SARs1 refers tothe SARs that produced extreme space weather events including ≥X5.0 flares, ground level events (GLEs) andsuper geomagnetic storms (SGSs: Dst≤ −250 nT), while SARs2 did not produce extreme space weather events.The total number of SARs1 and SARs2 are 32 and 19, respectively. The statistical results show that 34.4%, 65.6%and 78.1% of the SARs1 appeared in the ascending phase, descending phase and in the period from two yearsbefore to the three years after the solar maximum, respectively, while 52.6%, 47.4% and 100% of the SARs2appeared in the ascending phase, descending phase and in the period from two years before to the three years aftersolar maximum, respectively. The Carrington longitude distribution of the SARs1 shows that SARs1 in thelongitudinal scope of [0,150°] produced ≥X5.0 flares and GLEs, while only the SARs1 in the longitude range of[150°,360°] not only produced ≥X5.0 flares and GLEs, but also produced SGSs. The total number of SARsduring a SC has a good correlation with the SC size. However, the largest flare index of a SAR within a SC has apoor correlation with the SC size, implying that the number of SARs in a weak SC will be small. However, aweak SC may have a SAR that can produce very strong solar flare activities.
Evaluation of Mental Health Status of the Pregnant Women Working in Hospitals During Covid-19 Era
Authors: Eslamzadeh Mahboubeh , Najjari Bita , Emadzadeh Maryam , Feyzi Zhaleh , Modaresi Farzaneh , Mirzaeian Sara , Yazdi Aazam Sadat Heydari 
Published: November 2021

Abstract
Background Women represent the majority of the healthcare workforce and many of these women are probably pregnant and working at the front-line during COVID-19 outbreak. In COVID-19 crisis healthcare workers experienced excessive issues and challenges that made them vulnerable for getting various mental health disorders. We aimed to evaluate the mental health state among pregnant health care workers in COVID-19 era. MethodsThis cross-sectional study included 64 pregnant clinical residents, general physicians, nurses and other medical staff who worked in the hospitals in Iran, from August 2020 to December 2020 using an anonymous online survey available on Google Form platform. The online survey consisted of socio demographic questions, pregnancy-related questions, and some questions about medical/drug history, The Persian versions of General Health Questionnaire – 28 (GHQ-28), and Corona Disease Anxiety Scale (CDAS) were also fulfilled by the participants.ResultsThe mean age and gestational age of participants were 31.3±3.9 years, and 24.5±10.1 weeks. Psychological problems were moderate (total score GHQ-28> 40) in 9.4% (n:6) of the pregnant health care workers (PHCW). The frequency of moderate/severe problems in the four subclasses, including somatic symptoms, anxiety/insomnia, social dysfunction, and depression were 9(14.1%), 25(39.0%), 7(10.9%), and 10(15.6%), respectively. According to CDAS, 26.5% of participants had moderate to severe COVID-19 related anxiety. COVID-19 related anxiety and its subclasses, physical and psychological, were significantly correlated with GHQ-28 total score and its subclasses, including somatic symptoms, anxiety/insomnia, social dysfunction, and depression among pregnant health care workers (P<0.01).ConclusionAccording to the results of the current study, over 25% of the pregnant health care workers have moderate to severe COVID-19 related anxiety; so it is crucial for policymakers to focus on the mental health of the pregnant health care workers during COVID-19 pandemic. This study has major implications on health care practice. Further studies are needed to draw a clear picture of mental health problems among PHCW.
Muscle Contributions to Pre-Swing Biomechanical Tasks Influence Swing Leg Mechanics in Individuals Post-Stroke during Walking
Authors: Brough Lydia G. , Kautz Steven A. , Neptune Richard 
Published: November 2021

Abstract
Background

Successful walking requires the execution of the pre-swing biomechanical tasks of body propulsion and leg swing initiation, which are often impaired post-stroke. While excess rectus femoris activity during swing is often associated with low knee flexion, previous work has suggested that deficits in propulsion and leg swing initiation may also contribute. The purpose of this study was to determine underlying causes of propulsion, leg swing initiation and knee flexion deficits in pre-swing and their link to stiff knee gait in stroke survivors.
Methods

Musculoskeletal models and forward dynamic simulations were developed for individuals post-stroke (n=15) and neurotypical participants (n=5). Linear regressions were used to evaluate the relationships between peak knee flexion, braking and propulsion symmetry, and individual muscle contributions to braking, propulsion, knee flexion in pre-swing, and leg swing initiation.
Results

27% of individuals post-stroke had higher plantarflexor contributions to propulsion and 47% had higher vasti contributions to braking on their paretic leg relative to their nonparetic leg. Higher gastrocnemius contributions to propulsion were correlated to paretic propulsion symmetry (p=0.005) while soleus contributions were not. Higher vasti contributions to braking in pre-swing predicted lower knee flexion (p=0.022). The rectus femoris and iliopsoas did not directly contribute to lower knee flexion acceleration in pre-swing compared to contributions from the vasti. However, for some individuals with low knee flexion, during pre-swing the rectus femoris absorbed more power and the iliopsoas contributed less power to the paretic leg. Total muscle-tendon work done on the paretic leg in pre-swing was not correlated to knee flexion during swing.
Conclusions

These results emphasize the multiple causes of propulsion asymmetry in individuals post-stroke, including low plantarflexor contributions to propulsion, increased vasti contributions to braking and reliance on compensatory mechanisms. The results also show that the rectus femoris is not a major contributor to knee flexion in pre-swing, but absorbs more power from the paretic leg in pre-swing in some individuals with stiff knee gait. These results further highlight the heterogeneity of the post-stroke population and the need to identify individual causes of propulsion and knee flexion deficits to improve rehabilitation outcomes.
Hydrogen Enhanced the Myogenic Differentiation of Adipose Mesenchymal Stem Cells Through P38 MAPK Signaling Pathway
Authors: Fei Wenyong , Pang Erkai , Hou Lei , Dai Jihang , Liu Mingsheng , Wang Xuanqi , Xie Bin , Wang Jingcheng 
Published: November 2021

Abstract
Purpose: This study aims to clarify the systems underlying regulation and regulatory roles of hydrogen in the myogenic differentiation of adipose mesenchymal stem cells (ADSCs). Materials and methods: In this study, ADSCs acted as an in vitro myogenic differentiating mode. First, the Alamar blue Staining and mitochondrial tracer technique were used to verify whether hydrogen could promote cell proliferation. In addition, this study assessed myogenic differentiating markers (e.g., Myogenin, Mhc and Myod protein expressions) based on the Western blotting assay, analysis on cellular morphological characteristics (e.g., Myotube number, length, diameter and maturation index), RT-PCR (Mhc and Myod mRNA expression) and Immunofluorescence analysis (Desmin, Myosin and β-actin protein expression). Lastly, to verify the myogenic differentiating system of hydrogen, Western blotting assay was performed to detect p38 and p-p38 proteins expressions. Results: Hydrogen can remarkably enhance the proliferation of ADSCs in vitro by increasing the number of single-cell mitochondria and by up-regulating the expression of myogenic biomarkers (e.g., Myod, Mhc and myotube formation). The expressions of both p38 and p-p38 were up-regulated by hydrogen. The differentiating ability was suppressed when the cells were cultivated in combination with SB203580 (p38 MAPK signal pathway inhibitor). Conclusions: The present study initially indicated that hydrogen can promote myogenic differentiation via the p38 MAPK pathway. Thus, the mentioned results present insights into myogenic differentiation and are likely to generate one potential alternative strategy for skeletal muscle related diseases.
Tooth Loss During Supportive Periodontal Care in a Strict Non-surgically Treated Cohort – a Retrospective Data Analysis
Authors: Bartha Valentin , Mohr Judith , Krumm Boris , Herz Marco , Wolff Diana , Petsos Hari 
Published: November 2021

Abstract
Background: This retrospective study aimed to evaluate tooth loss (TL) within a strict non-surgically treated patient cohort after supportive periodontal care (SPC) of 2.5-10.7 years.Methods: Data for non-surgically treated patients were checked for: complete periodontal examination data at baseline (T0), after active periodontal therapy (T1), and after ≥2.5 years of SPC (T2); Smoking, diabetes mellitus, age (at least 18 years), plaque and gingival indices, bleeding on probing (BOP), percentage of residual pockets, SPC adherence, and number of SPCs were assessed as risk factors for TL.Results: 132 patients were included (76 female, mean age 56.7±10.3 years), mean T1–T2: 4.5±1.6 years. 26.5% of all patients lost 118 teeth (0.5 teeth/patient, 0.12 teeth/patient/year). Plaque and bleeding parameters: mean plaque control record (PCR): 59.77±28.07%, mean papilla bleeding index (PBI): 47.46±34.12%, mean BOP: 33.46±21.52%. SPC duration (p=0.013) and T2 BOP (p=0.048) were identified as patient-related risk factors for TL.Conclusion: The strictly non-surgical approach within the reported cohort was characterized by elevated BOP, PBI, and PCR scores, possibly highlighting the lack of surgical intervention and regular SPC as negative effect. An apparently low TL rate could be observed. Duration of SPC and BOP (T2) were identified as risk factors for TL.