bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-324-5P Functions as a Novel Serum Biomarker and Modulates Breast Cancer Malignant Activity
Authors: Zhang Jiling , Zhang Yanyan , Lv Shanmei , Wang Huiying , Gao Lu , Dong Xuejun 
Published: December 2021

Abstract
Introduction: The best management practices for patients with breast cancer（BC） involve early detection and deterioration prevention. Therefore, it is important to study the occurrence of BC and its development mechanism and explore more sensitive diagnostic and prognostic indicators.Objective: Our study explored the significance and the serum miR-324-5p application in the diagnosis and prognosis of BC and evaluated possible molecular mechanisms.Methods: MiR-324-5p expressions in the serum and tissue of patients with BC were observed by real-time fluorescence quantitative polymerase chain reaction (PCR). Next, the miR-324-5p diagnostic ability in BC was evaluated by the receiver operating characteristic curve. Kaplan-Meier analyzed the relationship between expression of miR-324-5P and prognosis. The Cox regression model evaluated the serum miR-324-5P prognostic value in predicting disease-free survival (DFS) and overall survival (OS). In functional studies, cell counting Kit-8 assay and Cloning experiment were employed to assess BC cells’ proliferation level. Transwell assays and wound healing assays were performed to determine the effect of miR-324-5p on BC cell invasion and migration. In addition, the flow cytometry test was conducted to determine the effect of miR-324-5p on the apoptotic rate of BC cells. Finally, bioinformatics tools were employed to detect the potential target genes of miR-324-5p in BC.Results: miR-324-5p expression is up-regulated in in BC cells, which is related to tumor stage（p=0.0291)and age( p=0.0278). In addition, a BC diagnosis based on serum miR-324-5p levels had an 66% sensitivity, 78% specificity, and the area under the curve was approximately 0.7581. DFS (p=0.027) and OS (p<0.017) in the low miR-324-5P expression group were higher than those in the high expression group. The tumor node metastasis staging、Ki-67 and miR-324-5P were independent prognostic factors for the survival of patients with BC. In addition, miR-324-5p had a positive effect on BC cell invasion and proliferation, thereby preventing apoptosis. Finally, bioinformatics synthesis showed that the miR-324-5p target genes in BC are cysteine-rich hydrophobic domain 1 (CHIC1) and kruppel-like factor 7 (KLF7).Conclusion: MiR-324-5p may perform its oncogenic functions by interacting with the CHIC1 and KLF7 genes, and its existence in serum could serve as a diagnostic and prognostic biomarker of BC.
Performance and Feasibility of Therapeutic Vibrating Mesh Nebulizer for Ventilation Lung Scan
Authors: Chang Chih-Yung , Yang Bang-Hung , Ke Chien-Chih , Hsu Jing-Long , Jhou Rong-Hong , Chang Wen-Yi , Peng Nan-Jing , Liu Ren-Shyan 
Published: December 2021

Abstract
This study aimed to evaluate the performance of a therapeutic vibrating mesh-type nebulizer for the pulmonary delivery of radioaerosols for lung scintigraphy in healthy subjects. Six healthy subjects (mean age of 28.7 ± 6.2 y) inhaled 2 mL of Tc-99m diethylenetriaminepentaacetic acid (DTPA) and normal saline solution (20 mCi) via the therapeutic vibrating mesh nebulizer (DK010, DELBio, Taipei, Taiwan). The nebulizer’s mass median aerodynamic diameter (MMAD) is between 2.3 μm and 5.0 μm (3.47 ± 0.37 μm) and the nebulization rate is greater than 0.2 ml/min. Scintigraphy was performed to count radioaerosols in the regions of interest to determine the total and regional lung deposition and extrathoracic airway deposition of aerosols, penetration of aerosols, and radioactivity count balance. The total lung deposition of aerosols was 21.2 ± 5.2% (% ex-valve dose), 27.4 ± 8.0% (% ex-device dose) and 13.8 ± 4.1% (% initial dose) in nebulizer. The extrathoracic airway deposition was 4.8 ± 1.1%. The radioactivity count balance was 5.4 ± 3.0%. The ratio of outer vs inner lung deposition (O/I ratio, or penetration index) was 1.89 ± 0.55. The delivery efficiency and the penetration of aerosols to the peripheral lung achieved by the DELBio DK010 vibrating mesh-type nebulizer are similar to the commercialized jet-type nebulizers dedicated for radioaerosol lung scintigraphy nebulizer. The therapeutic vibrating mesh-type nebulizer (DELBio DK010) is feasible for radionuclide lung ventilation scintigraphy.
Quantitative Analysis of Vegetation Dynamics in Response to Climatic Factors and Human Activities in Southwest China
Authors: Yang Cuiping , Wang Yongqiang , Wu Jiujiang , ma xiaoyi 
Published: December 2021

Abstract
We determined the time scale of normalized difference vegetation index (NDVI) response to drought and used trend and correlation analyses to explore the spatial and temporal variability characteristics of the NDVI and SPEI and their sensitivity to climatic factors in southwest China from 2000 to 2020. We used a partial derivative approach to calculate the contributions of six climatic factors and human activities to the interannual variation in the NDVI. The results demonstrated that from 2000 to 2020, the annual mean NDVI in southwest China showed a slight decreasing trend at a rate of 0.0001 y−1. The NDVI had the highest sensitivity to the standardized precipitation and evapotranspiration index on a 12-month time scale. The NDVI exhibited a 1-year delayed response to drought. The SPEI has the highest sensitivity to precipitation. The percentage of pixels with a positive correlation between NDVI and precipitation, mean temperature, temperature difference, mean relative humidity, mean wind speed, and sunshine duration in the study area was 31.73%, 46.81%, 35.49%, 25.76%, 39.36%, and 39.89%, respectively. The average contributions of these six climatic factors to the interannual variation of NDVI were 0.00029, 0.00046, −0.00007, 0.00007, 0.0008, and 0.00001 y−1, respectively. The NDVI had the highest sensitivity to mean temperature and the lowest sensitivity to mean relative humidity. The average contributions of climatic factors and human activities to interannual variability in southwest China were 0.00156 and 0.00012 y−1, respectively. The positive influence of climatic factors on the NDVI was stronger than that of human activities. This study provides a theoretical basis for the sustainable management of the regional ecological environment.
Hybrid Selection Based Multi/Many-Objective Evolutionary Algorithm
Authors: Dutta Saykat , Mallipeddi Rammohan , Das Kedar Nath 
Published: December 2021

Abstract
In the last decade, numerous Multi/Many-Objective Evolutionary Algorithms (MOEAs) have been proposed to handle Multi/Many-Objective Problems (MOPs) with challenges such as discontinuous Pareto Front (PF), degenerate PF, etc. MOEAs in the literature can be broadly divided into three categories based on the selection strategy employed such as dominance, decomposition, and indicator-based MOEAs. Each category of MOEAs have their advantages and disadvantages when solving MOPs with diverse characteristics. In this work, we propose a Hybrid Selection based MOEA, referred to as HS-MOEA, which is a simple yet effective hybridization of dominance, decomposition and indicator-based concepts. In other words, we propose a new environmental selection strategy where the Pareto-dominance, reference vectors and an indicator are combined to effectively balance the diversity and convergence properties of MOEA during the evolution. The superior performance of HS-MOEA compared to the state-of-the-art MOEAs is demonstrated through experimental simulations on DTLZ and WFG test suites with up to 10 objectives.
Food Insecurity and NSAP Use Among Sexual Minority People: Analysis of a Population-Based Sample From National Health Interview Survey, 2017
Authors: Tree Jennifer M. Jabson , Russomanno Jennifer , Bartmess Marissa , Anderson Joel 
Published: December 2021

Abstract
Background

Food insecurity is a pressing public health problem. Lesbian, gay, and bisexual (LGB) people are at risk for food insecurity, yet this issue remains grossly understudied among this population. The purpose of this study was to add to the existing literature surrounding food insecurity and the use of Federal food assistance programs (SNAP) among LGB people.
Methods

This study used publicly available, de-identified data from the 2017 National Health Interview Survey (NHIS). Primary variables were sexual orientation, food security status, and receipt of SNAP. Food security was assessed using the 10-item USDA Family Food Security measure.
Results

In our sample, people who identified as bisexual had the highest rates of food insecurity (23.8%, n = 76). Female sexual minorities were 52% more likely to experience food insecurity (aOR = 1.518, 95% CI 1.105–2.087, p = .01) and 44% more likely to report household SNAP assistance than their heterosexual counterparts (aOR = 1.441, 95% CI 1.025-2.028, p = .03). SNAP partially mediated the association between sexual orientation and food insecurity for LGB females.
Conclusions

Our findings add to the growing empirical evidence documenting food insecurities among sexual minority adults. Our results reiterate the need for sexual orientation to be included in nationally representative federal food security measures.
The Centres for Disease Control Light Trap (CDC LT) and The Human Decoy Trap (HDT) Compared to The Human Landing Catch (HLC) for Measuring Anopheles Biting in Rural Tanzania
Authors: Namango Isaac Haggai , Marshall Carly , Saddler Adam , Ross Amanda , Kaftan David , Tenywa Frank , Makungwa Noely , Odufuwa Olukayode , Ligema Godfrey , Ngonyani Hassan , Matanila Isaya , Bharmal Jameel , Moore Jason , Moore Sarah , Hetzel Manuel 
Published: December 2021

Abstract
BackgroundThe intensity of vector mosquito biting is an important measure for malaria epidemiology and control. The human landing catch (HLC) is an effective entomological surveillance tool, but is labour-intensive, expensive and raises safety issues. The Centres for Disease Control light trap (CDC LT) and the human decoy trap (HDT) are less costly and exposure-free alternatives. This study compared the CDC LT and HDT against the HLC for measuring Anopheles (An.) biting in rural Tanzania and assessed their suitability as HLC proxies.MethodsIndoor mosquito surveys using HLC and CDC LT and outdoor surveys using HLC and HDT were conducted in 2017 and in 2019 in Ulanga, Tanzania in 19 villages, with one trap per house per night. Species composition, sporozoite rates and the numbers of mosquitoes caught by different trap types were compared. Aggregating the data by village and month, the Bland-Altman approach was used to assess agreement. ResultsOverall, 66,807 Anopheles funestus and 14,606 An. arabiensis adult females were caught from 6,013 CDC LT, 339 indoor HLC, 136 HDT and 195 outdoor HLC collections. Overall, the CDC LT caught fewer malaria vectors than indoor HLC: An. arabiensis (Adjusted rate ratio (Adj.RR) =0.35 (95% confidence interval (CI):0.27-0.46)) and An. funestus (Adj.RR=0.63(95%CI:0.51-0.79)). HDT caught fewer malaria vectors than outdoor HLC: An. arabiensis (Adj.RR=0.04(95%CI:0.01-0.14)) and An. funestus (Adj.RR=0.10(95%CI:0.07-0.15)). The bias and variability of the ratios of geometric mean mosquitoes caught by CDC LT and HDT relative to HLC collections for the same village-month were dependent on mosquito densities. The relative efficacies of both CDC LT and HDT declined with mosquito abundance. The variability in the ratios was substantial for low HLC counts and decreased as mosquito abundance increased. CDCLT caught a higher proportion of infected An. arabiensis and An. funestus than HLC, and HDT caught no infected mosquitoes.ConclusionsIf caution is taken in appreciation of its limitations, the CDC LT is suitable for use in routine entomological surveys and may be preferable for measuring sporozoite rates for Afrotropical mosquitoes. Use of HLC is useful to estimate human exposure to mosquitoes for estimating Entomological Inoculation Rate (EIR). The present design of the HDT is not amenable for use to conduct large-scale entomological surveys.
WITHDRAWN: Effect of Different Temperatures on the Life Table Parameters of Rhyzobius Lophanthae Blaisdell (Coleoptera: Coccinellidae)
Authors: KAYAHAN Ali , AHMED Qasim Hussein , KARACA İsmail 
Published: December 2021

Abstract
The purple scale predator, Rhyzobius lophanthae Blaisdell (Coleoptera: Coccinellidae) is known as coccidophagous ladybird predator, and effective against scales’ insects. The present study aimed to evaluate the optimum temperature for the species to be more efficient. In this study, the life table parameters of R. lophanthae were determined on different temperatures at 4, 16, 18, 20, 22, 24, 26, 28, 30 and 32 °C and 60% RH, by calculations using RmStat-3 software according to Euler-Lotka equation. The results showed that the intrinsic rates of increase (rm) were 0.016, 0.022, 0.030, 0.052, 0.056, 0.068, 0.120, 0.142, 0.132, 0.021 females/females/day, respectively, while the net reproductive rates (R0) were 7.082, 9.514, 11.960, 50.906, 54.150, 49.525, 56.883, 80.944, 31.149, 1.882 females/females/generation, respectively. The mean generation times (T0) were 125.966, 104.602, 84.009, 75.742, 71.511, 57.568, 33.801, 30.866, 25.978, 30.759 days, respectively. Total productivity rates (GRR) were 34.865, 39.210, 48.216, 201.990, 209.469, 166.207, 177.779, 303.751, 105.751, 12.622 egg/female, respectively. The study concluded that 26-30 °C was the optimum temperature range for the efficient role of R. lophanthae under laboratory conditions. From the results, it is still needed to do more studies on the interactions of pests, predators with environmental conditions.
Evaluation of an Ayurvedic formulation in clinical recovery of COVID-19 patients: A placebo controlled pilot study among moderate-severe patients
Authors: Arun Abhishek , Gupta Amit , Subramanian Saumya , Kanchibhotla Divya 
Published: December 2021

Abstract
Introduction: The COVID-19 pandemic is a global health crisis that has affected millions of people worldwide. Apart from measures taken to reduce the transmission of virus and provide symptomatic relief to patients, a potent drug targeted against the virus is required to manage the disease. Traditional medicines with an array of phytochemicals and potent anti-viral properties, can provide alternate solution in the therapeutic cure of COVID-19.Objective: The present study investigates the efficacy of an Ayurvedic formulation, NOQ19, on the rate of recovery and clinical improvement among moderate-severe COVID-19 infected patients who were not on a ventilator or in intensive care unit.Methods: A placebo controlled randomized study design, with 1:1 ratio between the intervention and placebo arms was adopted. The participants were provided their respective intervention along with the standard of care treatment. A follow up was conducted on Day 7 and Day 10 to assess the rate of recovery and clinical improvement. Rate of recovery was the primary outcome measured and was determined by a negative result on the RT-PCR test. The secondary outcomes included clinical improvement among patients and were measured using blood biomarkers.Results: The NOQ19 arm had a higher percentage of population who turned RT-PCR negative on Day 7 (23%) when compared to the placebo arm (11%). Further subgroup analysis demonstrated that for both moderate and severe patients, there were a greater percentage of population who were RT-PCR negative in the NOQ19 arm compared to the placebo arm at Day 7: (NOQ-19: 22.22%, Placebo: 14.29% for moderate patients), (NOQ19: 23.08% Placebo: 0% for severe patients). By Day 10, both populations depicted an almost similar percentage of the population who were RT- PCR negative. Both the groups demonstrated an improvement in blood biomarkers, although the improvement was greater in the NOQ19 arm. None of the patients in the intervention arm reported any adverse events.Conclusion: The results of this pilot RCT indicates that NOQ19 along with standard of care treatment can be considered as an effective therapy for COVID-19 and aid early recovery from the disease.
Fever Among Preschool Aged Children: A Cross-Sectional Study Assessing Lebanese Parents’ Knowledge, Attitudes and Practices on Pediatric Fever Assessment and Management
Authors: Sakr Fouad , Akiki Zeina , Akel Marwan , Malaeb Diana , Dabbous Mariam , Salameh Pascale 
Published: December 2021

Abstract
Background
The existing literature demonstrates insufficient parental knowledge around fever, and international guidelines suggest studying parents’ perceptions and practices in treating febrile sickness. This study investigated parental knowledge, attitudes and practices toward fever in preschool children to help address all pointed gaps and provide information with the aim of supporting clinical reports for parental education.
Methods
A cross-sectional study design was used to explore parental experiences with fever. Participants were recruited randomly from schools all over Lebanon targeting the preschool divisions to include parents of children aged 5 years or less. An electronic self-administered questionnaire was sent to the parents through the schools’ emails and e-learning mobile applications, with a separate message encouraging parents to take time to fill carefully the survey.
Results
A total of 733 parents were included in the study. Only 44% identified fever correctly according to the recognized definition by international guidelines. A significant association between parents’ knowledge of antibiotics and years of parenting experience was found (ORa = 4.23, 95% CI 1.41-12.68, P=0.01). Other sociodemographic factors that were significantly associated with parents’ knowledge of antibiotics were age (ORa = 3.42, 95% CI 1.09-10.73, P=0.036) and education level (ORa = 7.99, 95% CI 3.71-17.23, P<0.001). Greater than 75% usually give their children antipyretics without consulting a doctor. Approximately one-quarter of parents (26.3%) consulted different doctors at the same time, of which more than half (58.4%) had received different medical information.
Conclusions
This research determines deficiencies in parents’ knowledge of fever with some malpractices in its management. It provides insight for healthcare providers to empower parental experiences by offering the necessary information to enhance general outcomes of febrile sickness.
Genomic Insights into the Diversity of Non-Coding RNAs in Bacillus Cereus Sensu Lato.
Authors: Gonçalves Kátia B. , Appel Renan J. Casarotto , Boas Laurival A. Vilas , Cardoso Priscilla F. , Boas Gislayne T. Vilas 
Published: December 2021

Abstract
Bacillus cereus sensu lato is a group of bacteria of medical and agricultural importance in different ecological niches and with controversial taxonomic relationships. Studying the composition of non-coding RNAs (ncRNAs) in several bacterial groups has been an important tool for identifying genetic information. However, to date, no comparative genomics study of ncRNA has been performed in this group. Thus, this study aimed to identify and characterize the set of ncRNAs from 132 strains of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis to obtain an overview of the diversity and distribution of these genetic elements in these species. We observed that the number of ncRNAs differs in the chromosomes of the three species, but not in the plasmids, when species or phylogenetic clusters were compared. The prevailing functional/structural category was Cis-reg and the most frequent class was Riboswitch. However, in plasmids, the class Group II intron was the most frequent. Also, nine ncRNAs were selected for validation in the strain B. thuringiensis 407 by RT-PCR, which allowed to identify the expression of the ncRNAs. The wide distribution and diversity of ncRNAs in the B. cereus group, and more intensely in B. thuringiensis, may help improve the abilities of these species to adapt to various environmental changes.
Pseudoretinoblastoma: Distribution Based on Gender, Age, and Laterality
Authors: Mirzayev Ibadulla , Gündüz Ahmet Kaan , Ateş Funda Seher Özalp , Tetik Diğdem 
Published: December 2021

Abstract
Purpose: To investigate the distribution of pseudoretinoblastoma (PSRB) cases based on gender, age, and lateralityMethods: The clinical records of 607 patients (851 eyes) who were referred for suspicion of retinoblastoma between October 1998 and May 2021 were retrospectively evaluated. Patients were stratified by age as follows: ≤1 year, >1-3 years, >3-5 years, and >5 years.Results: PSRB conditions were detected in 190/607 (31.3%) patients. Of 190 patients, 129 (67.9%) were males and 61 (32.1%) were females (p=0.001). The 3 most common diagnoses were persistent fetal vasculature (PFV; 16.3%), Coats disease (15.3%), and optic nerve head drusen (ONHD; 5.3%). In males, the 3 most common diagnoses were Coats disease (20.2%), PFV (14.0%), and chorioretinal coloboma (6.2%). The 3 most common diagnoses in females included PFV (21.3%), retinal dysplasia, congenital glaucoma, and combined hamartoma (each accounting for 6.6%). PFV was the most common diagnosis in ≤1 year old patient group (26.6%). Coats disease and PFV were the most common diagnoses in >1-3 years old patient group (16.7%, for each diagnosis). Coats disease was the most common diagnosis in >3-5 years old (30.8%) and >5 years old patient groups (13.1%). PSRBs were unilateral in 121/190 (63.7%) patients and bilateral in 69/190 (36.3%). The most common unilateral and bilateral diagnoses were Coats disease (24.0%) and PFV (24.6%) respectively.Conclusion: In our study, 31.3% of patients referred for suspicion of retinoblastoma received the diagnosis of PSRB. PSRB spectrum encompasses different diseases, the distribution of which differ depending on gender, age, and laterality.
The Anti-platelet and Anticoagulant Potential of Isorhamnetin and Its New Derivatives Isolated From Sea Buckthorn Fruits in Whole Blood
Authors: Rolnik Agata , Skalski Bartosz , Zuchowski Jerzy , Stochmal Anna , Olas Beata 
Published: December 2021

Abstract
Blood platelets play a crucial role in hemostasis, the process responsible for keeping blood flowing in the circulatory system. However, unnecessary platelet activation can lead to aggregation at the site of atherosclerotic plaque rapture and the formation of a thrombus, which promotes atherothrombotic diseases. Various dietary components, such as phenolic compounds, are known to demonstrate antiplatelet and anticoagulant properties, and it is possible that these could form an important element in the prophylaxis and therapy of cardiovascular diseases. Our present study examines the biological activity of isorhamnetin (compound 1) and two isorhamnetin derivatives, compound 2 (3-O-beta-glucoside-7-O-alpha-rhamnoside) and compound 3 (3-O-beta-glucoside-7-O-alpha-(3”’-isovaleryl)-rhamnoside), isolated from the phenolic fraction of sea buckthorn fruit, against human washed blood platelets and human whole blood in vitro. The anti-platelet and anticoagulant potential was determined using (A) flow cytometry, (B) the thrombus-formation analysis system (T-TAS) and (C) colorimetry. The tested flavonoids demonstrated anticoagulant and anti-platelet potential, including anti-adhesive activity, with these effects being more intense in compound 2 than isorhamnetin. Compound 2 inhibited GPIIb/IIIa and P-selectin expression on blood platelets from whole blood, and demonstrated anti-adhesion properties in washed blood platelets and anti-coagulant potential in whole blood, measured by T-TAS.