bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Population Growth and Mortality of Windu Shrimp (Penaeus monodon) in the Juata Water of Tarakan City, Indonesia
Authors: Salim Gazali , Handayani Kun Retno , Indarjo Agus , Ransangan Julian , Rizky Riky , Prakoso Lukman Yudho , Pham Yen Thi Hong 
Published: April 2021

HighlightThe sex ratio of tiger prawns in the waters of female groceries is 1.08 times higher than that of males.The growth characteristic of male and female tiger prawns is negative allometry, but the male and female body shapes are fat.Fishing mortality, capture mortality, exploitation rate, female tiger prawns are higher than males, but total mortality and natural mortality male tiger prawns are higher than females.The male infinitive growth is longer than the female.AbstractThe windu shrimp (Penaeus monodon) is an economically important fishery species in the waters of Tarakan. The shrimp is highly demanded for frozen seafood industry that makes its market price range attractive from Rp. 50,000 (USD 3.37) to Rp. 70,000 (USD 4.72)/kg. This can cause the species to be potentially exploited. However, due to lack of data, the exploitation status of the windu shrimp is unknown. Hence, the present study was carried out to determine the absolute growth, mortality, and exploitation level of the windu shrimp in Juata waters of Tarakan. Sampling of the shrimp using experimental trawling net was conducted four times between June and August 2020 in the waters of Tarakan. Morphometric data of the windu shrimps including sex, total length, carapace length, and total weight were recorded and analyzed. The results showed that the sex ratio of male and female tiger prawns was 1:1.08. The maximum growth of male and female shrimps was estimated at 26.875 and 21.435 cm respectively. The size of the male shrimps caught during the sampling ranged between 8.7 and 22.5 cm, and was estimated to be at 13 to 67 days old respectively. Maximum growth of male shrimp was estimated at 26.875 cm that could be achieved within 411 days. The size of female shrimp caught during the sampling ranged from 9.8 to 19.3 cm with an estimated age of 21 to 87 days respectively. The maximum growth for female shrimp was estimated at 21.435 cm, and achievable within 377 days. The mortality of male and female shrimps was not significantly different at 110.3% and 110.1% respectively. The fishing mortality for male and female shrimps was recorded at 19% and 22.5% respectively. The level of exploitation of male and female shrimps was slightly different at 17.2% and 20.5%, respectively. Finally, the natural mortality for male and female shrimps was at 91.3% and 87.5%, respectively.
Bioaccumulation of some Heavy Metals in Red mullet (Mullus barbatus) and Common pandora (Pagellus erythrinus) in Zliten Coast, Libya
Authors: Ighwela Keri Alhadi , Al-Kazaghly Ragab Farag , Hamid Mohamed 
Published: April 2021

Highlight Investigated in heavy metal bio-accumulation in red mullet (Mullus barbatus) and Common pandora (Pagellus erythrinus) collected in Zliten Coast, Libya.Based on the samples collected, the muscle tissue of fish species may be considered suitable for human consumption.The metal concentration levels observed were in range of acceptable limits proposed by regulations of WHO and FAO.AbstractThe current study aimed to determine the heavy metal concentration like as copper (Cu), zinc (Zn), lead (Pb), cadmium (Cd) and mercury (Hg) in the liver, gills, gonad and muscles in two fish species (red mullet (Mullus barbatus), and common Pandora (Pagellus erythrinus) collected from the Zliten coast in Libya. The levels of heavy metals were determined by using an atomic absorption spectrophotometer (AAS). The results showed that the concentration of heavy metals in the sampled fishes less than the permissible limits for human consumption. In addition, the bioaccumulation for Cu, Pb, Cd, and Hg by both fish species was: liver >gills > gonads > muscles, while Zn follows the sequence: gonads > liver > gills > muscles.
The Length-Weight Relationship and Condition Factors of Bullet Tuna Landed at the Tanjung Luar Fishing Port, Indonesia
Authors: Asrial Evron , Arapat Yasir , Hadi Usma Kurniawan , Kalih Lalu A.T.T.W.S , Liliyanti Mita Ayu , Rosadi Erwin , Khasanah Ruly Isfatul , Rathnayake Ishani Nelunika 
Published: April 2021

HighlightBullet tuna (Auxis rochei Risso 1810) has been identified (measured, weighed) and analyzed.The resource status of bullet tuna has been estimated based on body shape and age group.LWR curve is implemented to describe and express the age group of bullet tuna (immature, adult/mature, old)This study has resulted the composition and ratio of bullet tuna that is suitable to be caught, fit for consumption and worthy of maturationAbstractBullet tuna (Auxis rochei Risso 1810) or tongkol lisong (Indonesian) is important for human consumption and health as well as an income source for fishers and coastal communities. Its utilization problems are over exploitation, catch domination by immature groups, and the threatened stock security of the bullet tuna (BLT). This research aimed to determine the status of the prospective parent of bullet tuna caught in the Lesser Sunda region, particularly from the Alas Strait to the Indian Ocean, West Nusa Tenggara (WNT). Meanwhile, the data were collected from July to September 2020 (2nd transitional season) at the Tanjung Luar Fishing Port, East Lombok and a dependent survey method was adopted with sampling, interview, observation, and documentation techniques. Fork length and body weight data were used to estimate the length and weight relationship, growth pattern, condition factor, fish age group, and catch worth. The primary and secondary data were processed quantitatively using several equations. The growth pattern of bullet tuna was minor allometric (b = 2.875), worth selling (K > 1.00), and the majority was adult fish or mature group. The LWR model is . This condition indicates the bullet tuna is worth catching and worth spawning, so that it is eligible to be a potential broodstock candidate to support a sustainable management of BLT fisheries. Furthermore, fishers have applied the code of conduct for responsible fisheries (CCRF) in supporting the sustainable development goals.
The Effectiveness of Filopaludina javanica and Sulcospira testudinaria in Reducing Organic Matter in Catfish (Clarias sp.) Aquaculture Wastewater
Authors: Lailiyah Shofiyatul , Arfiati Diana , Hertika Asus Maizar Suryanto , Arum Neni Diah Kusumaning , Noviya Catur Budi 
Published: April 2021

HighlightCatfish aquaculture wastewater contains organic matter that exceeds the quality standard of 68.75 mg / L and 72.48 mg / L.Tutut Jawa snail (F. javanica) can reduce organic matter in waste by up to 92%.Susuh Kura snail (S. testudinaria) can reduce organic matter in waste by up to 87%.Catfish aquaculture wastewater treatment with snails only takes 8 hours. Abstract The residual or wastewater from the cultivation process that is discharged directly into common waters can reduce the quality of the waters because it contains a lot of organic material from organism feces and feed residue, so it needs management first. The purpose of this study was to determine the ability of Javanese snails and freshwater gastropods to reduce total organic matter in catfish culture waste. The research method used was the experiment with 2 factor Factorial completely randomized design, namely the measurement time (4, 8, 12, and 16 hours) and the abundance of the Tutut Java snail and freshwater gastropods (100%, 75%, 50%, 25%, and control) with 5 repetitions. Tutut Java snails were taken from the pond at UPR Sumbermina Dau and brought to the laboratory. The results obtained in the treatment with the addition of Tutut Java snail, the total organic matter content decreased at 75% snail density by 90% (from 68.75 mg / L to 5.31 mg / L) at the 12th hour. The treatment with the addition of Freshwater Gastropods at a density of 25%, the total organic matter content decreased by 87% (from 72.48 to 9.35 mg / L) at the 8th hour. Water quality during the study was in a condition that could tolerate the Tutut Java snails and freshwater gastropods. The conclusion is that the addition of Tutut Java snail and / or freshwater gastropods can reduce the total organic matter content to the water quality standard.
Heat Flow Transport Model by Gauss-Seidel Type Iteration Methods for Gas and Solid Materials
Authors: Rauf Nurlaela , Haeruddin Heryanto , Rahmat Roni , Tahir Dahlang 
Published: April 2021

Technological processes for modification of materials, deposition, and prevented fumes in the pyrolysis processes are used gases materials in the medium with vacuum pressure or atmospheric air pressure. Therefore, it is essential to understand heat flow transport for designing an eﬃcient reactor or find the substrate's excellent position in the reactor or furnace for growing materials. We evaluated the energy transfer phenomena in the form of temperature distribution and heat flow for various heating sources for the gases and solid materials by Gauss-Seidel equation. The thermal conductivity coefficient (k), number of heating sources, and position of heating sources show an essential parameter for transmitting the distribution of the heat. For high k value shows efficiently for heat transfer at low temperature due to the atom's position close each other. The heat also affects to the phonon and lattice vibration like a wave which successfully shows these phenomena in this study.
Unusual Osteoblastoma of the First Metatarsal Bone Treated by Total Resection and Fibular Autograft
Authors: Hammad Yazan , Alisi Mohammed , Elifranji Zuhdi , Mousa Khaled , Abuhassan Freih , Al-Mustafa Sahar 
Published: April 2021

Case:
A 25-year-old man presented with progressive pain and swelling of the left foot for 4 years. The images showed a contrast-enhanced mixed lesion (osteolytic and blastic) of the first metatarsal bone with surrounding soft-tissue edema. The differential diagnoses favored chronic osteomyelitis or neoplasia, most likely benign. Histopathology confirmed the diagnosis of osteoblastoma as immature bone trabeculae rimmed by osteoblasts appeared. Then, the patient was successfully treated with wide surgical excision of the first metatarsal bone and reconstruction using a nonvascularized fibular autograft. After 3 years of follow-up, the graft was well incorporated and no recurrence was encountered.

Conclusion:
Osteoblastoma is rare in metatarsals; only 12 cases have been reported. It may have similar clinical and radiological picture to osteomyelitis. Wide surgical excision followed by fibular autograft reconstruction is an effective treatment option.
The Impacts of On-farm Diversification to the Family Farm and the Intersection of Land Preservation through Public Planning in Ontario
Authors: Duesling Pamela 
Published: April 2021

Have you ever wondered what prompts Ontario family farmers to diversify, what land use planning policies allow for alternative uses on farms and how Ontario can continue to preserve agricultural lands as family farming continues to change? This primary research objective is to understand how on-farmdiversification impacts the family farm and what the intersection of on-farm diversification and land preservation is through public planning policy in Ontario. The 2016 OMAFRA Guidelines on PermittedUses in Ontario’s Prime Agricultural Areas was/is the first tool that provides family farmingentrepreneurs and municipal government planners opportunities to create on-farm diversified uses whilebalancing agricultural land preservation. This research will:

Endeavour to explore which rural municipalities are using this tool efficiently andeffectively; Discover if the Guidelines are assisting entrepreneurs and identifying bestpractices;
Identify if it is only prime agricultural lands that warrant these Guidelines for landpreservation; Acknowledge if the Guidelines are preserving prime agricultural lands; and
Propose changes to the Ontario planning policy framework regarding on-farm diversification andland preservation.

Overall, it is simply not enough to preserve agriculture land in Ontario, we must also preserve the family farmer Keywords: on-farm diversification; land preservation; family farm; Ontario planning policy
The Ideal Approach for Treatment of cT1N+ and cT2Nany Esophageal Cancer.: A NCDB Analysis
Authors: Huang Binhao , Chan Ernest G. , Pennathur Arjun , Luketich James D. , Zhang Jie 
Published: April 2021

Abstract
Background Neoadjuvant therapy followed by surgery is recommended for locally advanced esophageal cancer. With the inaccuracies of clinical staging particularly for cT1N+ and cT2Nany tumors, some have proposed consideration of surgery followed by adjuvant treatment. Our objective is to evaluate the efficacy of neoadjuvant therapy vs surgery followed by adjuvant therapy, and to identify the ideal sequence of treatment in patients with cT1N+ and cT2Nany tumors.Methods We performed an analysis utilizing the National Cancer Database (2006-2015) identifying all patients with cT1N+ and cT2Nany esophageal cancer undergoing esophagectomy and additional chemotherapy or radiotherapy. The treatment was stratified as: neoadjuvant therapy (NT), adjuvant therapy (AT) and combination therapy of neoadjuvant and adjuvant (CT) groups and outcomes were analyzed.Results We identified 2795 patients with 81.9% (n=2289) receiving NT, 10.2% (n=285) AT, and 7.9% (n=221) CT. There were no significant differences noted in survival among AT, NT, and CT group in cT1N+(P=0.376), cT2N-(P=0.436), cT2N+(P=0.261) esophageal cancer by multivariate analysis using Cox regression model. This relationship held true in both squamous cell carcinoma and adenocarcinoma. Conclusion In clinical T1N+, T2Nany patients, there was no evident superiority of NT over AT. Surgery followed by adjuvant therapy can be considered to be an alternative option in these patients. Further prospective studies are needed to validate these findings.
Five years surface ozone behaviour in a semi-rural location at Mohal-Kullu in the north-western Himalaya, India
Authors: Kuniyal Jagdish Chandra , Choudhary Sheetal , Sharma Priyanka 
Published: April 2021

Abstract
Surface ozone is one of the most important Green House Gases (GHGs). Five years (2011–2015) measurements of surface ozone (O3) and one of its precursors- oxides of nitrogen (NOx) were carried out at a semi-rural location, Mohal (77.12°E, 31.91°N, 1154 m amsl) in the north-western Indian Himalaya. The concentration of O3, NO, NO2 and NOx was measured maximum 74.6 ± 23.2 ppb in 2013, 27.5 ± 7.5 ppb in 2013, 51.8 ± 13.2 ppb in 2013, and 60.8 ± 13.2 ppb in 2012, respectively. Seasonally, O3 concentration was highest during summer while lowest in monsoon. The O3 concentration shows unimodal peak while its precursors show bimodal peaks. A reasonable decrease in percent change was found in terms of O3 (-13), NO2 (-6), and NOx (-3) due to imposing regulations imposed by local government in compliance with the order of Hon’ble National Green Tribunal (NGT) of the country in 2015. However, NO (9) is increasing due to vehicular activities in the nearby area which is one of the famous tourist spots. Washout effect due to increasing rainfall by 8% might have also affected O3 concentrations. Biomass burning for heating and cooking purposes and forest fire for tender livestock forage and transported gaseous pollutants from the Middle East countries and Indo-Gangetic Plain (IGP) could be major contributors of surface ozone and its precursors. HYSPLIT air-mass back trajectories drawn for external ozone sources showed the maximum air masses reached the study location either with the western desert countries or IGP polluted regions.
Hepatocyte-derived exosomal microRNAs orchestrate vascular inflammation and endothelial function: insights into molecular mechanisms of trimethylamine-N-Oxide in atherosclerosis
Authors: Liu Xiang , Shao Yijia , Tu Jiazichao , Sun Jiapan , Li Lifu , Tao Jun , Chen Jimei 
Published: April 2021

Abstract
Background: Trimethylamine-N-oxide (TMAO) has been proved to be a new proatherogenic compound for promoting vascular inflammation and endothelial dysfunction. Hepatocyte-derived exosomes played an important role in the regulation of vascular inflammation and endothelial function. Since TMAO is produced in the liver, hepatocytes may be the first potential target of TMAO. However, it is not clear whether TMAO can directly stimulate normal hepatocytes to produce exosomes so as to mediate the motivating effects of TMAO on inflammation and endothelial dysfunction.Methods: The hepatocytes were cultured and treated with TMAO at a physiological concentration for 24 hours (TMAO-Exos). The untreated group served as the control (Control-Exos). The exosomes were isolated from the culture supernatant and then added to the human aortic endothelial cells (HAECs) for 48 hours. The mRNA expressions of inflammatory cytokines and caspase-3 were determined by qPCR and cell apoptosis was evaluated by using the Hoechst 33342 staining solution. The miRNA profile in the exosomes were detected using an RNA-sequencing strategy. The miRNA-mRNA network was predicted, and the biological functions of the target genes were annotated by using bioinformatics methods.Results: TMAO-Exos were able to promote the expressions of inflammatory cytokines and HAECs apoptosis. Moreover, miRNAs carried by the TMAO-Exos were quite different from that in the Control-Exos, including miR-92a-3p, miR-103-3p and miR-122-5p, etc. Further analysis showed that these differentially expressed miRNAs were predicted to target genes such as Mapk8, Casp9, Mapk10, Bcl2l11, Ikbkg and Akt1, which were supposed to be involved in the signal pathways related to vascular inflammation and endothelial function.Conclusions: These novel results provided evidence that TMAO could indirectly talk to vascular endothelial via promoting hepatocytes to secreting exosomes that carried important genetic information, which may give a new insight into the interactions between liver and vasculature in the atherogenesis caused by TMAO. New intervention targeting this cellular crosstalk may be feasible and effective in the prevention and treatment of TMAO-induced atherogenesis.
Cigarette smoke or motor vehicle exhaust exposure induces PD-L1 upregulation in lung epithelial cells in COPD model rats.
Authors: Chen Rui , Li Defu , Wang Lingling , Dong Jiahui , Xiong Richeng , Guo Zhenhui , Huang Wenjie 
Published: April 2021

Abstract
Cigarette smoking and ambient air pollution are common risk factors for COPD and lung cancer. However, the underlying mechanism between COPD prevalence and lung cancer is remained elusive. In this study, we established rat COPD model through exposure to cigarette smoke (CS) or motor vehicle exhaust (MVE). The model rats developed COPD-like phenotypes, manifested as lung functions decline, lung inflammation and airway remodeling. The Programmed death-ligand 1 (PD-L1), a factor contributing to immune escape of tumor cells, was overexpressed in lungs from COPD model rats. The inflammatory responses and PD-L1 upregulations were also observed in cultured human bronchial epithelial cells BEAS-2B upon treatment with cigarette smoke extract (CSE) or diesel related particulate matter 2.5 (PM2.5, SEM1650b). Furthermore, both CS/CSE and MVE/PM2.5 induced ERK1/2 activation, a kinase mediating PD-L1 upregulation in premalignant bronchial cells or NSCLC cells, in COPD rats’ lungs or in BEAS-2B cells. Activations of STAT1/3, which was reportedly associated with PD-L1 expression in lung tumors, were detected in lungs from CS- or MVE-induced COPD model rats. However, CSE preferred to activate STAT3 and PM2.5 inclined to activate STAT1 in BEAS-2B cells. Therefore, we proposed that cigarette smoke and ambient air pollution elevate the risk of lung cancer in COPD patients by increasing PD-L1 expression in lung epithelial cells, suggesting the effects of immunesuppressive microenvironment on promoting tumorigenesis in COPD patient’s lung.
Sevoflurane post-conditioning alleviated hypoxic-ischemic brain injury in neonatal rats by inhibiting endoplasmic reticulum stress-mediated autophagy via IRE1 signalings
Authors: Niu Jiayuan , Wu Ziyi , Xue Hang , Zhang Yahan , Gao Qiushi , Li Chang , Zhao Ping 
Published: April 2021

Abstract
Post-conditioning with sevoflurane, a volatile anesthetic, has been proved to be neuroprotective against hypoxic-ischemic brain injury (HIBI). Our previous research showed that autophagy is over-activated in a neonatal HIBI rat model, and inhibition of autophagy confers neuroprotection. There is increasing recognition that autophagy can be stimulated by activating endoplasmic reticulum (ER) stress. Herein, we purposed to explore: i) the association of ER stress with autophagy in the setting of neonatal HIBI; and ii) the possible roles of ER stress-triggered autophagy, as well as IRE1 signaling in the neuroprotection of sevoflurane post-conditioning against neonatal HIBI. Seven-day-old rats underwent ligation of the left common artery, and a subsequent 2 hour hypoxia (8% O2 / 92% N2). The association of ER stress with autophagy was examined by ER stress inducer (tunicamycin), 4-PBA (ER stress inhibitor), or 3-MA (autophagy inhibitor). Rats in the sevoflurane post-conditioning groups were treated with 2.4% sevoflurane for 30 minutes after HIBI stimulation. The roles of ER stress-mediated autophagy, as well as the IRE1-JNK-beclin1 signaling cascade in the neuroprotection afforded by sevoflurane were explored by ER stress inducer (tunicamycin) and the IRE1 inhibitor (STF-083010). HIBI over‑activated ER stress and autophagy in neonatal rats. HIBI-induced autophagy was significantly aggravated by tunicamycin but blocked by 4-PBA; however, HIBI-induced ER stress was not affected by 3-MA. Sevoflurane post-conditioning significantly alleviated ER stress, autophagy, cell apoptosis, and cognitive impairments, which were remarkably abolished by tunicamycin. Also, tunicamycin blocked sevoflurane-induced downregulation of IRE1-JNK-beclin1 signaling pathway. Whereas, IRE1 inhibitor could reverse the effects of tunicamycin. ER stress contributes to autophagy induced by HIBI. Furthermore, sevoflurane post-conditioning significantly protects against HIBI in neonatal rats by inhibiting ER stress-mediated autophagy via IRE1-JNK-beclin1 signaling cascade.
Cloning and identification of a new repressor of 3,17β-Hydroxysteroid dehydrogenase of Comamonas testosteroni
Authors: Xie Weiqi , Xia Qin , Chen Ling , Xiong Guangming , Gao Yuwei , Yu Yuanhua , He Xiuxia 
Published: April 2021

Abstract
3,17β-hydroxysteroid dehydrogenase (3,17β-HSD) is a key enzyme that can degrade steroid compounds in Comamonas testosteroni. Tetracycline repressor (TetR) family, repressors existing in most microorganisms, may play key roles in regulating the expression of 3,17β-HSD. Previous reports showed that three TetR genes were located in the contig58 of C. testosteroni ATCC 11996 (GenBank: AHIL01000049.1), among which the first TetR gene encoded a potential repressor of 3,17β-HSD by sensing environmental signals. However, whether the other proposed TetR genes acts as repressors of 3,17β-HSD is still unknown. In the present study, we cloned the second TetR gene and analyzed the regulation mechanism of the protein on 3,17β-HSD using electrophoretic mobility shift assay (EMSA), gold nanoparticles (AuNPs)-based assay, and loss-of-function analysis. The results showed that the second TetR gene was 660-bp, encoding a 26 kD protein, which could regulate the expression of 3,17β-HSD gene via binding to the conserved consensus sequences located 1100-bp upstream of the 3,17β-HSD gene. Furthermore, the mutant strain of C. testosteroni with the second TetR gene knockout has good biological genetic stability, and the expression of 3,17β-HSD in the mutant strain is slightly higher than that in the wild type under testosterone induction, suggesting the mutant can efficiently degrade steroids as carbon sources. The mutant generated in this study can be used to treat environmental pollution caused by steroid hormones.