bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro effects of citral on the human myometrium: Potential adjunct therapy to prevent preterm births
Authors: Muñoz‐Pérez Víctor Manuel , Ortiz Mario I. , Salas‐Casa Andrés , Pérez‐Guerrero Jessica , Castillo‐Pacheco Narmi , Barragán‐Ramírez Guillermo , Hernándes‐Alejandro Mario 
Published: January 2021

AbstractBackgroundPremature infants contribute to infant morbidity and mortality especially in low resource settings. Information on tocolytic and/or anti‐inflammatory effects of several plant extracts, such as citral, could help prevent preterm birth cases and reduce the number of preterm infants. The aim of this study was to evaluate the in vitro tocolytic and anti‐inflammatory effect of citral on myometrial tissues of the human uterus.MethodsMyometrial samples from uteri obtained after hysterectomy were used in functional tests to evaluate the inhibitory effect of citral on PGF‐2α induced contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to citral in human myometrial homogenates were measured by ELISAs. Forskolin was used as a positive control. The anti‐inflammatory effect of citral was determined through the measurement of two pro‐inflammatory cytokines, tumor necrosis factor‐α (TNFα) and interleukin (IL)‐1β, and the anti‐inflammatory cytokine IL‐10, in human myometrial explants stimulated with lipopolysaccharide (LPS).ResultsCitral was able to induce a significant inhibition of PGF‐2α induced contractions at the highest concentration level (p < .05). Citral caused a concentration‐dependent increase in myometrial cAMP levels (p < .05) and a concentration‐dependent decrease in LPS‐induced TNFα and IL‐1β production, while IL‐10 production increased significantly (p < .05). The anti‐inflammatory and tocolytic effects induced by citral could be associated with an increase in cAMP levels in human myometrial samples.ConclusionThese properties place citral as a potentially safe and effective adjuvant agent in preterm birth cases, an obstetric and gynecological problem that requires urgent attention.
Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies
Authors: Weinguny Marcus , Klanert Gerald , Eisenhut Peter , Lee Isac , Timp Winston , Borth Nicole 
Published: January 2021

AbstractChinese hamster ovary (CHO) cells are the most extensively used mammalian production system for biologics intended for use in humans. A critical step in the establishment of production cell lines is single cell cloning, with the objective of achieving high productivity and product quality. Despite general use, knowledge of the effects of this process is limited. Importantly, single cell cloned cells display a wide array of observed phenotypes, which so far was attributed to the instability and variability of the CHO genome. In this study we present data indicating that the emergence of diverse phenotypes during single cell cloning is associated with changes in DNA methylation patterns and transcriptomes that occur during the subcloning process. The DNA methylation pattern of each analyzed subclone, randomly picked from all outgrowing clones of the experiment, had unique changes preferentially found in regulatory regions of the genome such as enhancers, and de‐enriched in actively transcribed sequences (not including the respective promoters), indicating that these changes resulted in adaptations of the relative gene expression pattern. The transcriptome of each subclone also had a significant number of individual changes. These results indicate that epigenetic regulation is a hidden, but important player in cell line development with a major role in the establishment of high performing clones with improved characteristics for bioprocessing.
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva
Authors: Zheng Wentao , Wang Xue , Chen Yuwei , Dong Yachao , Zhou Diao , Liu Ruxin , Zhou Haibo , Bian Xiaoying , Wang Hailong , Tu Qiang , Ravichandran Vinothkannan , Zhang Youming , Li Aiying , Fu Jun , Yin Jia 
Published: January 2021

AbstractMicrobial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01‐1 is a broad‐spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting, for which, genome manipulation tools are essential. We previously developed Pseudomonas‐specific phage‐derived homologous recombination systems for genetic engineering in four Pseudomonas species. Herein, we report the application of these recombineering systems in Pseudomonas parafulva CRS01‐1, along with structural elucidation and bioactivity evaluation of natural products. The Pseudomonas recombineering toolbox established before in different four species is efficient for genome mining and bioactive metabolite discovery from more distant species.
Keeping the inner voice inside the head, a pilot fMRI study
Authors: Stephane Massoud , Dzemidzic Mario , Yoon Gihyun 
Published: January 2021

AbstractIntroductionThe inner voice is experienced during thinking in words (inner speech) and silent reading and evokes brain activity that is highly similar to that associated with external voices. Yet while the inner voice is experienced in internal space (inside the head), external voices (one's own and those of others) are experienced in external space. In this paper, we investigate the neural basis of this differential spatial localization.MethodsWe used fMRI to examine the difference in brain activity between reading silently and reading aloud. As the task involved reading aloud, data were first denoised by removing independent components related to head movement. They were subsequently processed using finite impulse response basis function to address the variations of the hemodynamic response. Final analyses were carried out using permutation‐based statistics, which is appropriate for small samples. These analyses produce spatiotemporal maps of brain activity.ResultsReading silently relative to reading aloud was associated with activity of the “where” auditory pathway (Inferior parietal lobule and middle temporal gyrus), and delayed activity of the primary auditory cortex.ConclusionsThese pilot data suggest that internal space localization of the inner voice depends on the same neural resources as that for external space localization of external voices—the “where” auditory pathway. We discuss the implications of these findings on the possible mechanisms of abnormal experiences of the inner voice as is the case in verbal hallucinations.
Outcomes in refractory diffuse large B‐cell lymphoma: results from a multicenter real‐world study in China
Authors: Wang Shuo , Wang Li , Hu Jianda , Qian Wenbin , Zhang Xi , Hu Yu , Zhu Qi , Chen Bobin , Wu Depei , Chang Chung‐Chou H. , Xu Pengpeng , Zheng Xiaoyun , Wei Juying , Liu Yao , Cui Guohui , Tang Yong , Ma Yan , Huang Haiwen , Yi Hongmei , Zhao Weili 
Published: January 2021

AbstractBackgroundDiffuse large B‐cell lymphoma (DLBCL) patients refractory to rituximab‐based immunochemotherapy have a dismal prognosis. However, the definition of refractory DLBCL remains inconsistent and no large cohort study data is available from Asian countries. To validate the definition and outcomes of refractory DLBCL in China, we conducted a multicenter, retrospective cohort study.MethodsThe REtrospective AnaLysis of Treatment REspoNse of refractory DLBCL (REAL‐TREND) study was performed using real‐world data from 8 centers in China. DLBCL patients with curative intent were included in the REAL‐TREND dataset. Overall survival (OS) was estimated using the Kaplan‐Meier method and compared by the log‐rank test. Due to heterogeneity in response rates among different centers, the response rates of refractory patients were pooled using random‐effect models. Multivariate survival analysis was performed using the Cox regression model.ResultsA total of 2778 DLBCL patients diagnosed between January, 2010 and December, 2015 were enrolled to this study. After validating previous definitions, the SCHOLAR‐1 study was most suitable to define refractory DLBCL. The estimated 5‐year cumulative incidence of refractory patients was 20% (95% confidence Interval [CI] = 18%‐22%). After the determination of refractory disease, overall response rate and complete remission rate were 30% (95% CI = 22%‐38%) and 9% (95% CI = 4%‐15%), respectively. Patients with either no response to immunochemotherapy or relapse within 12 months after stem‐cell transplantation had inferior survival with a median OS of 5.9 months (95% CI = 5.5‐7.1 months) and 2‐year OS rate of 16% (95% CI = 12%‐20%). International prognostic index score 4‐5 (hazard ratio [HR] = 2.22; 95% CI = 1.47‐3.35), central nervous system relapse (HR = 1.43; 95% CI = 1.04‐1.97), and best response status (HR = 2.68; 95% CI = 1.42‐5.03 for partial remission. HR = 5.97, 95% CI = 3.21‐11.11 for stable disease/progressive disease) were independent unfavorable prognostic factors.ConclusionsThis is the first large‐scale Asian cohort study focusing on outcomes of refractory DLBCL. The definition of the SCHOLAR‐1 study identifies patients with homogenously inferior survival, thus is appropriate to select refractory DLBCL. Due to poor clinical outcomes in the rituximab era, patients with refractory DLBCL may be potential candidates for novel treatment modalities.
Efficacy and safety of sorafenib plus vitamin K treatment for hepatocellular carcinoma: A phase II, randomized study
Authors: Haruna Yoshimichi , Yakushijin Takayuki , Kawamoto Seiichi 
Published: January 2021

AbstractThe previous retrospective study suggested that dosing vitamin K may enhance the anticancer action of sorafenib against hepatocellular carcinoma. To confirm it, we performed a phase II, randomized, open‐label study. Patients with hepatocellular carcinoma were randomly assigned to receive sorafenib + vitamin K2 (menatetrenone, 45 mg daily, orally) or sorafenib only. Between 1 May 2012 and 1 May 2016, 68 patients were screened. Forty‐four eligible patients were assigned at a 1:1 ratio to each cohort. The objective response rate in the vitamin K‐dosed group was significantly higher than that in the sorafenib only group (27.3% vs 4.5%, respectively; p = 0.039). The median time of progression‐free survival was significantly extended in the vitamin K‐dosed group compared with the sorafenib only group (4.9 months vs 2.7 months, respectively; hazard ratio (HR), 0.44; 95% confidence interval (CI): 0.21–0.89; p = 0.018). Although there was no significant difference between the two groups in the median time of overall survival, patients in the vitamin K‐dosed group with a complete response or partial response achieved a significantly extended median time of overall survival compared with the other patients in the vitamin K‐dosed group or the patients in the sorafenib only group (26.1 months vs 9.0 months; HR, 0.34; 95% CI: 0.11–0.95; p = 0.046 or 11.5 months; HR, 0.16; 95% CI: 0.034–0.70; p = 0.006, respectively). Dosing vitamin K could augment the anticancer action of sorafenib against HCC.
Real world outcomes of combination and timing of immunotherapy with radiotherapy for melanoma with brain metastases
Authors: Moyers Justin T. , Chong Esther G. , Peng Jiahao , Tsai Hsin Hsiang Clarence , Sufficool Daniel , Shavlik David , Nagaraj Gayathri 
Published: January 2021

AbstractIntroductionImmunotherapy (IT) and radiotherapy (RT) have improved overall survival in patients with melanoma with brain metastasis (MBM). We examined the real‐world survival impact of IT and RT combination and timing strategies.Materials and MethodsFrom the facility‐based National Cancer Database (NCDB) data set, 3008 cases of MBM were identified between 2011 and 2015. Six treatment cohorts were identified: stereotactic radiosurgery (SRS) + IT, SRS alone, whole brain radiotherapy (WBRT) + IT, WBRT alone, IT alone, and none. Concurrent therapy was defined as IT given within 28 days before or after RT; nonconcurrent defined as IT administered within 28–90 days of RT. The co‐primary outcomes were propensity score‐adjusted overall survival by treatment regimen and overall survival by RT and IT timing.ResultsMedian overall survival (mOS) was performed for each treatment category; SRS +IT 15.77 m; (95%CI 12.13–21.29), SRS alone (9.33 m; 95%CI: 8.0–11.3), IT alone (7.29 m; 95%CI: 5.35–12.91), WBRT +IT (4.89 m; 95%CI: 3.65–5.92), No RT or IT (3.29 m; 95%CI: 2.96–3.75), and WBRT alone (3.12 m; 95%CI 2.79–3.52). By propensity score matching, mOS for SRS +IT (15.5 m; 95%CI: 11.5–20.2) was greater than SRS alone (10.1 m; 95%CI: 8.4–11.8) (p = 0.010), and median survival for WBRT +IT (4.6 m; 95%CI: 3.4–5.6) was greater than WBRT alone (2.9 m; 95%CI: 2.5–3.5) (p < 0.001). In the SRS +IT group, 24‐month landmark survival was 47% (95%CI; 42–52) for concurrent versus 37% (95%CI; 30–44) for nonconcurrent (p = 0.40).ConclusionThose who received IT in addition to WBRT and SRS experienced longer survival compared to RT modalities alone, while those receiving concurrent SRS and IT trended toward improved survival versus nonconcurrent therapy.
Argireline: Needle‐Free Botox as Analytical Challenge
Authors: Kluczyk Alicja , Ludwiczak Julita , Modzel Maciej , Kuczer Mariola , Cebrat Marek , Biernat Monika , Bąchor Remigiusz 
Published: January 2021

AbstractArgireline‐containing cosmetics attract public interest due to their confirmed reduction of facial wrinkles. Argireline is a peptide that works by inhibiting the release of neurotransmitters in the neuromuscular junction, producing a botox‐like effect. Therefore, it is used as a safe needle‐free alternative to botox treatment. In this work we investigated the presence of Argireline in cosmetic creams and sera by application of reversed phase liquid chromatography and tandem mass spectrometry (RP‐HPLC/MS and MS/MS). The analysis revealed the presence of argireline and its oxidized form in several different cosmetics. The methionine residue in Argireline sequence was indicated as oxidation point according to neutral loss MS studies. The developed sample preparation strategy minimizes and monitors methionine oxidation, bringing to our attention the question of impact of ingredients on the stability of cosmetic product.
Efficacy of the novel technique HIRANODOME in preventing distal embolization during endovascular treatment of femoropopliteal lesions
Authors: Fukagawa Tomoya , Hirano Keisuke , Mori Shinsuke , Yamawaki Masahiro , Kobayashi Norihiro , Tsutsumi Masakazu , Honda Yohsuke , Makino Kenji , Ito Yoshiaki 
Published: January 2021

AbstractObjectivesThe efficacy and clinical outcomes of a novel technique “HIRANODOME” (Interim hemostatic technique with HIgh pressure for Regional blood flow in the superficial femoral Artery, NOninvasive Distal protection Occlusion MEthod) in preventing distal embolization during endovascular treatment (EVT) of femoropopliteal lesions were evaluated.BackgroundDistal embolization of femoropopliteal lesions may worsen limb ischemia or cause limb loss. Conventional filter wires are cumbersome and expensive and may cause vessel injury. HIRANODOME can, therefore, be a feasible and noninvasive alternative.MethodsBetween April 2007 and August 2018, 94 consecutive patients who underwent EVT for femoropopliteal lesions along with anticipated distal embolization were identified. About 9 out of these 94 patients were excluded due to filter device use and 8 were excluded due to acute limb ischemia. Consequently, 77 patients using HIRANODOME for distal protection were included. HIRANODOME involved wrapping the Tometakun around the knee to enable external compression of the popliteal artery, thereby blocking the blood flow.The evaluation items were 30‐day mortality, 30‐day major amputation, prevention from distal embolization, and thrombus capture rate.ResultsThe mean age was 74.4 ± 10.3 years, 53.2% were men, 55.8% were diabetic, and 9.1% were on hemodialysis. The mean lesion length was 199.5 ± 94.4 mm; 79.2% were class C/D lesions (TASC II classification). The 30‐day mortality and major amputation rates were 1.9 and 1.0%, respectively. The rate of prevention of distal embolization or no‐flow/slow‐flow phenomenon was 93.4%. Capture of thrombus was observed in 66 lesions (62.3%).ConclusionsThe HIRANODOME technique was effective in preventing distal embolization during EVT of femoropopliteal lesions.
A case of porokeratotic adnexal ostial nevus misdiagnosed as wart
Authors: Kwon Bo Ram , Kim Rosa , Byun Ji Yeon , Choi You Won , Choi Hae Young , Lee Min Young 
Published: January 2021

AbstractPorokeratotic adnexal ostial nevus may be misdiagnosed as wart based on clinical appearance and morphology. An accurate diagnosis through skin biopsy is crucial, as is the inclusion of rare disorders such as porokeratotic adnexal ostial nevus in the differential diagnosis.
Reversible hypoxic‐ischemic encephalopathy post prolonged out‐of‐hospital cardiac arrest: A case series
Authors: Ata Fateen , Bint I Bilal Ammara , Tajelsir Abdalla Osman Omnia , Arif Muhammad Awais , Elhassan Mawahib , hamid Tahir , Al Suwaidi Jassim , Choudry Hassan , Abushahba Galal 
Published: January 2021

AbstractThis article highlights the possibility of positive outcomes associated with prolonged CPR and anoxic brain injury contesting the idea that such patients will invariably end up in a persistent vegetative state.
Novel Ferrocene‐Appended β‐Ketoimines and Related BF2 Derivatives with Significant Aggregation‐Induced Emission and Second‐Order Nonlinear Optical Properties
Authors: David Ezhumalai , Colombo Alessia , Dragonetti Claudia , Palanisami Nallasamy 
Published: January 2021

AbstractA series of new β‐ketoimines containing a ferrocene moiety and their BF2 complexes have been synthesized and structurally characterized. The solvatochromism of the β‐ketoimines was studied, putting in evidence a redshift with increasing solvent polarity. This positive solvatochromism can be attributed to a more polarized excited state compared with the ground state, due to intramolecular charge transfer (ICT) transitions. The β‐ketoimines exhibited weak emission, attributable to the excited‐state intramolecular proton transfer (ESIPT) phenomenon. This ESIPT effect is suppressed upon restriction of the keto‐enamine tautomerism, induced upon addition of BF3⋅OEt2, which afforded the related BF2 complexes, characterized by an enhancement of the fluorescence through the ICT effect. Both the β‐ketoimines and BF2 complexes exhibited significant aggregation‐induced emission behavior in mixtures of CH3CN/H2O, due to restriction of intramolecular rotation in the aggregated state. The frontier molecular orbital levels, ground‐ and excited‐state dipole moments (μg and μe), and the origin of electronic absorption spectra were studied by time‐dependent DFT calculations. The second‐order nonlinear optical (NLO) properties were determined by the electric‐field‐induced second‐harmonic generation technique. The μβ1907 values of the β‐ketoimines increased upon the formation of the related BF2 complexes, mainly due to an enhancement of the ground‐state dipole moment. The results presented here reveal that some of these novel compounds are excellent multifunctional candidates for NLO and luminescence applications.
Twisting versus Delocalization in CAAC‐ and NHC‐Stabilized Boron‐Based Biradicals: The Roles of Sterics and Electronics
Authors: Schmid Paul , Fantuzzi Felipe , Klopf Jonas , Schröder Niklas B. , Dewhurst Rian D. , Braunschweig Holger , Engel Volker , Engels Bernd 
Published: January 2021

AbstractInvited for the cover of this issue is Bernd Engels, Holger Braunschweig, Volker Engel and their coworkers at University of Würzburg. The image depicts bridged boron compounds which possess fascinating relationships between their composition and their geometrical and electronic structures, the latter ranging from closed‐shell to biradical triplet or singlet ground state. Read the full text of the article at 10.1002/chem.202004619.
Transition Metal‐Free N‐Arylation of Amino Acid Esters with Diaryliodonium Salts
Authors: Kervefors Gabriella , Kersting Leonard , Olofsson Berit 
Published: January 2021

AbstractA transition metal‐free approach for the N‐arylation of amino acid derivatives has been developed. Key to this method is the use of unsymmetric diaryliodonium salts with anisyl ligands, which proved important to obtain high chemoselectivity and yields. The scope includes the transfer of both electron deficient, electron rich and sterically hindered aryl groups with a variety of different functional groups. Furthermore, a cyclic diaryliodonium salt was successfully employed in the arylation. The N‐arylated products were obtained with retained enantiomeric excess.
Direct Synthesis of Unsymmetrical Dithioacetals
Authors: Bognar Sabine , van Gemmeren Manuel 
Published: January 2021

AbstractSabine Bognar and Manuel van Gemmeren from the WWU Münster have been invited to contribute the cover of this issue. The image depicts a volcano to symbolize the general theme of sulfur chemistry, whereas the piles of rubble reflect the selectivity of the reaction. Read the full text of the article at 10.1002/chem.202004835.
2‐Fluoro‐5‐nitrophenyldiazonium: A Novel Sanger‐Type Reagent for the Versatile Functionalization of Alcohols
Authors: Fischer Oliver , Heinrich Markus R. 
Published: January 2021

AbstractAs a novel Sanger‐type reagent, 2‐fluoro‐5‐nitrophenyldiazonium tetrafluoroborate enabled the versatile functionalization of primary and secondary aliphatic alcohols. Based on a mild nucleophilic aromatic substitution of the fluorine atom under unprecedented, base‐free conditions, the diazonium unit on the aromatic core of the resulting aryl‐alkyl ether could be employed for such diverse transformations as radical C−H activation and cyclization, as well as palladium catalyzed cross‐coupling reactions.
Battery‐Buffered Alkaline Water Electrolysis Powered by Photovoltaics
Authors: Becker Maik , Brauns Jörn , Turek Thomas 
Published: January 2021

AbstractThe combination of an alkaline water electrolyzer (AWE) with a battery system powered by photovoltaics (PV) for the production of green hydrogen is investigated. A model describes the power distribution between these three subsystems (AWE, battery and PV). Variation of AWE and battery power and capacity is carried out for two locations, to identify the most appropriate setup, where the highest energy usage and operating time can be reached. The battery helps to reduce the power level of the AWE. However, an estimation of the costs indicates that further optimization is necessary.
Cu‐Catalyzed Cascade Cyclization of Ketoxime Acetates and Alkynals Enabling Synthesis of Acylpyrroles
Authors: Xu Zhenhua , Xian Ning , Chen Hongbiao , Deng Guo‐Jun , Huang Huawen 
Published: January 2021

Main observation and conclusionA facile copper‐based catalytic system has been developed to enable efficient cyclization of methylketoximes and alkynals. This protocol provides a viable entry to synthetically and pharmaceutically useful 2‐acylpyrroles with a broad range of compatible functionalities. Mechanistically, a key acyl migration is probably involved that leads to the formation of N‐acyl pyrroles or otherwise NH pyrroles by further hydrolysis. More importantly, the present reaction system also gives an opportunity to realize three‐component pyrrole assembly by simple addition of carboxylic acid.