bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Adaptation and Rehabilitation Potential of A Family That Bring Up A Child With Health Limitations in The Conditions of A Parent Club
Authors: Chemerilova Irina , Kirilova Olga , Gavrilova Irina , Akyeva Nurgozel 
Published: June 2021

Abstract
Family adaptation to parenting and accompanying a child with disabilities is currently a serious social and psychological problem. This study is aimed at studying the development, adaptation and rehabilitation potential of a family raising a child with disabilities in an inclusive education. This study is also aimed at theoretical substantiation, experimental verification and confirmation of the possibilities to adapt and rehabilitate such families with the help of a parent support program based on the parent club of the Center for Psychological, Medical and Social Assistance. The methodological basis of the study was the systematic and active support to families in inclusive education. The research was carried out by analyzing scientific literature and using psychological techniques. The results of the study deepened the scientific understanding of the principles, mechanisms, methods of measuring the effectiveness and improving the process of accompanying a family raising a child with disabilities in inclusive practice. The article defines the essence and content of the adaptive and rehabilitation potential of a family raising children with disabilities, identifies the structural components; and provides diagnostic tools. The results of the experimental study prove that the actual problem of improving adaptive and rehabilitative potential of a family raising children with disabilities can be successfully solved with the help of a parental support program in a parent club.
α-Lipoic Acid Induces Intrinsic Apoptosis in Human Oral Squamous Carcinoma Cells
Authors: Duraisamy Revathi , Devaraj Ezhilarasan , Perumal Elumalai 
Published: June 2021

Abstract
Oral squamous cell carcinoma is one of the leading cancers in India and it is responsible for significant morbidity and mortality. α -lipoic acid, a co-factor for several metabolic enzymes, suppresses the tumor growth. In this study, we investigated the α-lipoic acid-induced cytotoxicity and apoptosis in human oral squamous carcinoma (SCC-25) cells. α-lipoic acid treatments were given to SCC-25 cells for 24 h and cell proliferation was evaluated by MTT assay. The reactive oxygen species expression was examined by dichloro-dihydro-fluorescein diacetate assay. Apoptosis-related morphological changes were detected by dual staining. Cytochrome c and RAS (H-Ras) expression was measured by dual staining and RT-PCR respectively. Intrinsic apoptosis-related markers are analyzed using qPCR.α-lipoic acid inhibited SCC-25 cell proliferation in a concentration-dependent manner. This treatment also increased intracellular reactive oxygen species expression and the percentage of apoptotic cells (up to 70% of the cell population). Dual staining further confirms cytochrome c cytosolic expression. The oncogene H-Ras protein and gene expression was also down-regulated upon α-lipoic acid treatment in SCC-25 cells. qPCR analysis further confirms α-lipoic acid-induced an upregulation of bax, Apaf-1, caspase 3 and − 9, pro-apoptotic gene expressions and downregulation of bcl-2, an anti-apoptotic gene expression. The present results suggest that α-lipoic acid has cytotoxic and pro-apoptotic potential and it also downregulates H-Ras oncogene expression in human oral squamous carcinoma cells. α-lipoic acid may have promising role in the treatment of human oral squamous carcinoma.
Control of Intramolecular Electron Transport Pathways by Varying Localized Electron Distribution
Authors: Zhou Dahai , Duan Ping , Zhou Yu , Li Chuan , Wang Yaping , Chen Lichuan , Wang Jin-Yun , Qu Kai , Tang Chun , Shi Jia , Liu Junyang , Zhang Qian-Chong , Chen Zhong-Ning , Hong Wenjing 
Published: June 2021

Abstract
The control of different electron transport pathways by quantum interference (QI) effects offers a unique opportunity for the modulation of electrical properties in molecular electronic devices and materials. In this work, we propose a chemical way to control the intramolecular electron transport pathways by the localization of the highest occupied molecular orbital (HOMO) distribution. The negative charge injection in para-carbazole by deprotonation exhibited a fourfold suppression of single-molecule conductance, while the conductance is almost the same for meta-carbazole before and after deprotonation. The flicker noise analyses and theoretical simulations revealed the localized distribution of HOMO on the para-carbazole center, leading to the appearance of destructive quantum interference (DQI) effect for the control of electron transport pathway. This strategy of reaction-induced orbital localization offers a new strategy for the control of charge transport through molecular devices and materials.
Mapping the pre and post seismic crustal stress heterogeneity analogous to 2016, Mw 7.8, Kaikoura quake, New Zealand
Authors: M. Anupama , P.S. Sunil 
Published: June 2021

Abstract
Heterogeneity of pre and post seismic stress states associated to any earthquake play a primary role in understanding the earthquake mechanism and hazard assessment of a seismically dynamic region. The Mw 7.8, November 14, 2016 Kaikoura, New Zealand earthquake offer an unprecedented possibility to observe the heterogeneity in stress field over a very complex fault system wherein subduction zone converges with the strike slip faults system. Here we report the pre and post seismic stress field asperity first time in terms of spatial and temporal variations of b-values associated to the Kaikoura main-shock. Pre seismic spatial disparity of b-value indicates the existence of two prominent low b-value clusters, one towards southwest closer to the epicenter and other to the north of the rupture zone. During co seismic period, owing to the stress release near the epicentral area, the pattern of prominent low b-value pattern has become negligible in the post seismic period. However, the pattern of low b-value in the north of the rupture zone remains similar in the post seismic period indicates the unreleased strain energy in the province. The temporal evaluation of the earthquakes frequency magnitude distributions over a period of two decades also showed an analogous pattern that the b-values were decreased considerably before the large earthquakes in the expanse, which could spawn a larger future earthquakes in the vicinity.
Prevalence of Placental Infection with Plasmodium Falciparum Detected by Polymerase Chain Reaction and Associated Risk Factors in Women After Delivered Ouagadougou (Burkina Faso)
Authors: Haffsatou Sawadogo , Zida Adama , ZONGO Cheikna , Soulama Issiaka , Sawadogp Marcel Patindoilba , Guiguemde Thierry , Sangaré Ibrahim , Sangaré Lassana , Traoré Yves , Ouedraogo-Traore Rasmata , Guiguememde Robert T. , Savadogo Aly 
Published: June 2021

Abstract
Background: Malaria is known to have a negative impact on pregnant women and their foetuses. This infection during pregnancy represents a major public health problem in tropical and subtropical regions. The aim of this study was to determine the prevalence and risk factor of Plasmodium falciparum in pregnant women the city of Ouagadougou (Burkina Faso). Methods: A cross-sectional study was conducted from April 2019 to March 2020 in four health districts within Ouagadougou, capital city. Samples were collected from the placenta from 531 women after delivered Plasmodium falciparum then by PCR. Results: The prevalence placental malaria with of Plasmodium falciparum was estimated at 7.53%. The status of unemployment and/ or the status of residence around the city of Ouagadougou represent risk of malaria infection.Conclusion: Malaria in pregnancy is responsible for several complications so emphasis should be placed on communication about malaria control in pregnancy and, the behavior of pregnant women and health workers as well.
Functional Modules Analysis and Hub Gene Prognostic Values Evaluation Based on Co-Expression Network in Gastric Cancer
Authors: Liu Yujia , Hu Xiaoping , Pan Zongfu , Jiang Yuchen , Guo Dandan , Xu Tong , Sun Jiao , Huang Ping , Zhang Yiwen 
Published: June 2021

Abstract
Background: Gastric cancer is one of the most common fatal disease worldwide, but its mechanism and therapeutic targets are still unclear. In this study, we have analyzed the differences in gene modules and key pathways in gastric cancer patients, then elaborated the mechanism and effective treatment of gastric cancer with microarray data from the gene expression omnibus(GEO) database.Methods: GEO2R tools were used to identify differential expression genes (DEGs), String database was employed to construct a protein-protein interaction (PPI) network. We imported the PPI network into the Cytoscape software to find key nodes, and employed statistical approach of MCODE to cluster genes. After that the ClueGO was used to enrich and annotate the pathways of key modules. To investigate the relationship between the upstream regulator and hub genes, the transcriptional regulatory network was built based on TFCAT database.Results: 63 characteristic genes of gastric cancer are involved in regulation of ECM-receptor interaction, focal adhesion and protein digestion and absorption. SPARC, FN1, BGN and COL1A2 are four key nodes relating to tumor proliferation and metastasis, and their expression were strongly associated with poor survival (p<0.05). 13 transcription factors including PRRX1 have remarkable changes in gastric cancer, which may play a key role in hub gene regulation.Conclusions: The present study defined the gene expression characteristics and transcriptional regulatory network that promote our understanding of the molecular mechanisms underlying the development of gastric cancer, and might provide new insights into targeted therapy and prognostic markers for the personalized treatment of gastric cancer.
Tetrahydropalmatinee alleviates diabetic neuropathic pain by inhibiting the inflammation via p38-MAPK signaling pathway in microglia
Authors: Cheng Lianzhi , Ma Junlong , Jiang Aijuan , Cheng Kai , Wang Fanjing , Chen Qian 
Published: June 2021

Abstract
Object: Exploring the effect of Tetrahydropalmatine (THP) on diabetic neuropathic pain (DNP) and its possible mechanism. Methods: The type 2 diabetic (T2DM) rat models were prepared by high-fat and high-sugar feeding combined with a single small-dose intraperitoneal injection of streptozotocin (STZ). When the mechanical withdrawal threshold (MWT) and the thermal withdrawal latency (TWL) of T2DM model rats decreased to less than 85% which were judged as DNP-bearing rats. After treatment with or without THP, the protein expression of hypertonic glycerol reactive kinase (p38), phosphorylated hypertonic glycerol-responsive kinase (p-p38) and OX42 (a specific marker of microglia) were detected by Western Blot and and the mRNA content of p38 and OX42 were detected by qRT-PCR. The expression of pro-inflammatory factors IL-1β, IL-6, TNF-α, as well as chemotactic factors and their receptors including CXCL1, CXCR2, CCL2 and CCR2 in spinal tissues were detected by ELISA. Serum FINS and GSP content were also detected by ELISA. Double-label immunofluorescence were used to observe the expression of OX42 and p-p38 in the spinal dorsal horn. Results: Results showed that THP inhibited microglial activation of spinal in DNP rats. And after THP intervention, the MWT and TWL of DNP rats decreased, the expression of p38, p-p38 and OX42 in the spinal cord tissues of rats was significantly reduced while the mRNA of p38 and OX42 also reduced. The expression of IL-1β, IL-6, TNF-α, CXCL1, CXCR2, CCL2 and CCR2 in the spinal cord tissues of rats was significantly reduced (P < 0.01). At the same time, THP significantly proved FINS, but did not affect FBG and GSP in DNP rats. Conclusions: THP significantly alleviates pain symptoms in DNP rats, and this effect may be achieved by inhibiting the inflammatory response caused by the activation of microglia mediated by the p38-MAPK signaling pathway.
Acute Cerebral Infarction in a patient of Myelodysplastic Syndrome: A Case Report and literature review
Authors: Chen Ke-Yang , Tao Lin-Shuang , Sun Ni , Chen Song-Fang , Hu Bei-Lei 
Published: June 2021

Abstract
Myelodysplastic syndrome (MDS) is a bone marrow failure syndrome characterized by cytopenia that results in infection and bleeding. However, there are few reports of cerebral infarction in MDS. In this case report we reported a Chinese female patient diagnosed MDS without drugs and an onset of acute cerebral infarction. Imaging examinations showed an ischemic stroke and further bone marrow aspiration identified MDS in the patient. Low dose aspirin and rehydration were used to improve symptom, as well as anti-epileptic drugs and rehabilitation. We also reviewed acute cerebral infarction associated with MDS from a total of three reported cases without drugs for the treatment of MDS. Our data provide further evidence that acute ischemic stroke might be associated with MDS, which may be due to complex chromosomal abnormality and inflammatory processes.
MRI-Based Demonstration of The Normal Glymphatic System in a Human Population: A Systematic Review
Authors: Lee Min Kyoung , Cho Se Jin , Bae Yun Jung , Kim Jong-Min 
Published: June 2021

Abstract
The glymphatic system has been described as the exchange between cerebrospinal fluid and interstitial fluid. Recently, many studies have demonstrated the presence of the glymphatic system based on MRI. The purpose of our study is to systematically review the studies demonstrating normal glymphatic system in a human population using MRI, and to find a detailed imaging protocol for the glymphatic system. We searched the MEDLINE and EMBASE databases to identify studies with human participants that contained MRI-based demonstrations of the normal glymphatic system. We extracted data on the imaging sequence, imaging protocol, and the targeted anatomical structures on each study. According to studies using contrast-enhanced MRI, peak enhancement was sequentially detected first in the CSF space, followed by the brain parenchyma, the meningeal lymphatic vessel (MLV), and finally the cervical lymph nodes, which corresponds to the glymphatic flow. Non-contrast flow-sensitive MRI showed similar results of glymphatic inflow from the CSF space to the brain parenchyma and efflux from the brain parenchyma to the MLV. Based on current systematic review, we recommended T1-weighted MRI using contrast material to visualize the glymphatic system. Our result can increase understanding of the glymphatic system and may lay the groundwork for future development of a standard imaging protocol.
Comparative transcriptome analysis uncovers cell wall reorganization and repressed cell division during cotton fiber initiation
Authors: Liu Wenyuan , Lv Yanjia , Li Xiaoyue , Feng Zongqin , Wang Lichen 
Published: June 2021

Abstract
Background

Tetraploid cotton plants serve as prime natural fiber source for the textile industry. Although various omics studies have revealed molecular basis for fiber development, a better understanding of transcriptional regulation mechanism regulating lint fiber initiation is necessary to meet global natural fiber demand.
Conclusions

Here, we aimed to perform transcriptome sequencing to identify DEGs (differentially expressed genes) in ovules of the cotton variety Xu142 and its fibreless mutant Xu142fl during early lint fiber initiation period. Totally, 5516 DEGs including 1840 upregulated and 3676 downregulated were identified. GO enrichment analysis revealed that the downregulated DEGs mainly associated with biological processes such as transcription related biosynthesis and metabolism, organic cyclic compound biosynthesis and metabolism, photosynthesis, and plant cell wall organization, with molecular functions involving transcription related binding, organic cyclic compound binding, and dioxygenase activity, while the upregulated DEGs were associated with DNA replication and phospholipid biosynthetic related processes. Among the 490 DEGs annotated as transcription factor genes 86.5% were downregulated in the mutant including the Malvaceae-specific MMLs, expression patterns of which were confirmed during the central period of lint fiber initiation. Investigation of the 20 genes enriched in the cell wall organization revealed that 17 were EXPA coding genes. Overall, we suggest that lint fiber initiation is a complicated process involving cooperation of multiple transcription factor families, which might ultimately lead to the reorganization of the cell wall and terminated cell division of the differentiating fiber initials.
Biodegradable Antimicrobial Agent/Analgesic/Bone Morphogenetic Protein-Loaded Nanofibrous Fixators for Bone Fracture Repair
Authors: Yu Yi-Hsun , Lin Yu-Ting , Hsu Yung-Heng , Chou Ying-Chao , Ueng Wen-Neng W. N. , Liu Shih-Jung 
Published: June 2021

Abstract
Background

Post-operative infection and pain management are two critical aspects that are of great concern to orthopedic surgeons. Although there are several protocols available to deal with these issues, they are fraught with complications such as cartilage damage, cardiovascular and neurological intoxication, and systemic adverse responses. Therefore, it is necessary to develop safe and effective perioperative protocols. In the present study, antimicrobial agents/analgesics/growth factor-embedded biodegradable hybrid fixators (polycaprolactone fixator + poly[lactide-co-glycolide] sheath-core structured nanofibers) for bone fracture repair were designed. These fixators were fabricated using solution-extrusion three-dimensional printing and electrospinning. The in vitro and in vivo release of the incorporated vancomycin, ceftazidime, lidocaine, and bone morphogenetic protein-2 (BMP-2) was evaluated. The in vivo efficacy of the biomolecule-loaded nanofibrous fixators was investigated in rabbit rib-fracture models.
Results

The nanofibrous fixators were shown to release vancomycin, ceftazidime, and lidocaine in a sustained manner in both in vitro and in vivo conditions and protected BMP-2 from burst release. The implantation of these hybrid fixators around the fractured rib significantly improved animal activities and bone union indicating that the inclusion of analgesic in the fixator effectively reduced the post-surgical pain and thereby helped in recovery.
Conclusions

The novel biomolecule-loaded nanofibrous hybrid fixators resulted in excellent therapeutic outcomes. They may be effective in the repair of rib fractures in clinical settings and may help deal with surgical complications such as infection, non-union, and intolerable post-operative pain.
METTL3 Facilitates Colorectal Carcinoma Progression via Regulating m6A-CRB3-Hippo Axis
Authors: Liu Feng , Xiao Xiaoli , Pan Jiashu , Xu Ruohui , Dai Liang , Zhu Mingzhe , Xu Hanchen , Xu Yangxian , Zhao Aiguang , Zhou Wenjun , Dang Yanqi , Ji Guang 
Published: June 2021

Abstract
Background

Colorectal carcinoma (CRC) is the third most common cancer and the second most common cause of cancer-related death worldwide. RNA N6-methyladnosine (m6A) and methyltransferase-like 3 (METTL3) play an important role in cancer. However, the roles of m6A and METTL3 in CRC progression are still elusive.
Methods

Adenoma and CRC samples were applied to detect m6A and METTL3 levels, and tissue microarrays were performed to evaluate their associations with survival of CRC patients. The biological functions of METTL3 were investigated by CCK8, wound healing and transwell assays. M6A epitranscriptomic microarray, RNA stability and luciferase reporter assays were performed to explore the mechanism of METTL3 in CRC.
Results

m6A and METTL3 levels were significantly upregulated in both adenoma and CRC tissues, and the CRC patients with high m6A or METTL3 level had both shorter overall survival. METTL3 knockdown markedly inhibited the proliferation, migration and invasion of CRC cells. M6A epitranscriptomic microarray revealed that the cell polarity regulator Crumbs3 (CRB3) was the downstream target of METTL3. METTL3 knockdown markedly inhibited the degradation of CRB3 mRNA to increase the CRB3 expression. In addition, CRB3 level was also markedly reduced in both adenoma and CRC tissues, and the CRC patients with high CRB3 level had higher overall survival and disease free survival. CRB3 knockdown significantly promoted the proliferation, migration and invasion of CRC cells. Finally, CRB3 knockdown inhibited Hippo pathway, and increased nuclear localization of YAP.
Conclusions

The m6A and METTL3 levels were significantly increased in both adenoma and CRC tissues. The CRC patients with high m6A or METTL3 levels had shorter overall survival. Mechanistically, METTL3 regulated the initiation and progression of CRC via regulating m6A-CRB3-Hippo pathway.
Relationship of Objectively Measuring Physical Activity and Sitting Time on Plasma Lipid Metabolism During Pregnancy
Authors: Chen Hanqing , Zhang Casper J. P. , Fang Xuanbi , Tan Zijian , Yan Ni , Ming Wai-Kit , Wang Zilian 
Published: June 2021

Abstract
Background: Physical activity (PA) was commonly associated with pregnancy outcomes, including plasma lipids. We hypothesized that women with more PA would improve plasma lipid status than those with less PA during pregnancy. This study aimed to investigate the association of PA and sitting time with blood lipids in the first two trimesters. Methods: A prospective study was performed among 197 cases aged 18 to 40 recruited from a regional university affiliated hospital in south China. accelerometer were used to measure maternal PA in two trimesters (first measurement within 10th to 14th week of gestation and second measurement within 20th to 24th weeks of gestation; 7 consecutive days for each measurement). Maternal fasting venous blood was collected to test plasma lipids (cholesterol, triglyceride, low- and high-density lipoprotein [LDL and HDL]) in each of two trimesters.Results: A total of 197 cases completed this study. After adjusting for age and pre-pregnancy BMI, moderate PA of the first trimester was inversely linear associated with triglycerides in early pregnancy (β=-0.231, P=0.002). And it was also inversely significant linear association with triglycerides of the second trimester (β=-0.196, P=0.006). Moderate PA in early pregnancy showed a positively non-significant linear association with HDL levels of the first trimester (β=0.134, P=0.068). However, sitting time of the second trimester were positively non-significant associated with total cholesterol (β=0.126, P=0.080）and LDL levels (β=0.124, P=0.082）of the second trimester.Conclusions: Sitting time of the second trimester would positively associated with total cholesterol and LDL levels of the mid pregnancy. Higher moderate PA of early pregnancy was associated with lower triglycerides, and this effect lasting to the mid pregnancy. Therefore, pregnant women should be encourage to increase moderate PA and decrease sitting time from early pregnancy to improve lipid status.