bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Zirconium Metal-Organic Framework with SOC Topological Net for Catalytic Peptide Bond Hydrolysis
Authors: Wang Sujing , Ly Hong Giang T , Wahiduzzaman Mohammad , Dovgaliuk Iurii , Tissot Antoine , Maurin Guillaume , Parac-Vogt Tatjana , Serre Christian 
Published: June 2021

Abstract
The discovery of nanozymes for selective fragmentation of proteins would boost the emerging areas of modern proteomics, however, the development of efficient and reusable artificial catalysts for peptide bond hydrolysis is challenging. Here we report the detailed catalytic properties of a microporous zirconium carboxylate metal-organic framework, MIP-201, in promoting peptide bond hydrolysis in a simple dipeptide, as well as in horse-heart myoglobin (Mb) protein that consists of 153 amino acids. We demonstrate that MIP-201 features an excellent catalytic activity and selectivity, a good tolerance toward reaction conditions covering a wide range of different pH values, and importantly, an exceptional recycling ability associated with easy regeneration process. Taking into account the excellent catalytic performance of MIP-201 and its other advantages such as 6-connected Zr6 cluster active sites, the green, scalable and cost-effective synthesis, and an outstanding chemical and architectural stability, our finding suggests that MIP-201 may be a promising and practical alternative to the current commercially available catalysts for peptide bond hydrolysis.
Using Multi-Fractal and Joint Multi-Fractal Theories to Characterise the Spatial Variability of Soil Particle Size Distribution in an Underground Coalmine Area
Authors: Li Sijia , Wang Jinman , Zhang Jiarui , Zhang Min 
Published: June 2021

Abstract
Underground coal mining leads to serious surface deformation, which negatively affects the physical properties of soils Soil particle size distribution (PSD) is one of the most basic soil physical characteristic that influences other important properties such as soil hydraulics and thermodynamics. Understanding the spatial variability of the soil PSD in subsided land can provide targeted guidance for land reclamation. In this study, we conducted a quantitative study on the spatial variability of the soil PSD in the Pingshuo mining area on the Loess plateau, Shanxi Province in China, and explored the effects of subsidence and reclamation on the soil PSD. A plot experiment, including one unmined plot (UMP), one subsided plot (SUP), and one reclaimed plot (RCP), was performed in Anjialing No.3 underground coal mine in the, Pingshuo mining area. Four multi-fractal parameters of the soil PSD—D(0), D(1), Δα(q), and Δf(α)—were analyzed at the three sample sites. The joint multi-fractal method was carried out to analyze the spatial correlation of the soil PSD to further reveal the impacts of coal mining subsidence and land reclamation on the soil PSD. The multi-fractal method can reflect the local non-uniformity and heterogeneity of the soil PSD, while the joint multi-fractal approach can illustrate the correlation of the soil PSD between different soil depths. The range and spatial variability of the soil PSD increased due to coal mining subsidence and the impact of subsidence on the spatial disturbance of the surface soil PSD was greater than that of the deeper layers. The spatial correlation of clay in subsided land was larger than those of unmined land and reclaimed land, whereas, for silt and sand, the correlation was smaller. Land reclamation decreased the spatial variability of the soil PSD, which was near that of the unmined land after reclamation.
Physical Activity Levels, Exercise Preferences, and Exercise Barriers in Children and Adolescents after Cancer Treatment
Authors: Kim Ji Young , Yoo Samuel , Yeon Su Jin , Min Ji Hee , Kim Dong-il , Lee Ji Won , Han Jung Woo , Lyu Chuhl Joo , Jeon Justin 
Published: June 2021

Abstract
Purpose: The purpose of the current study was to explore physical activity (PA) and exercise participation, exercise preferences, and barriers to PA in childhood cancer survivors.Methods: We surveyed 120 childhood cancer survivors in childhood and adolescence from the pediatric oncology center in South Korea between March and August 2017. The modified Exercise & Quality of Life questionnaire, Korea Youth Risk Behavior Web-based Survey, and Godin Leisure-Time Questionnaire were used to assess PA levels, preferences, and exercise barriers.Results: Among 120 participants (72 boys, 48 girls) whose average age at the time of the survey was 14.57 ± 3 years and average age at diagnosis was 8.22 years, only 6.9% of male participants and 2.1% of female participants met the PA recommendations for children (at least 60 min of moderate PA per day). The most preferred sporting activities included soccer, basketball, strengthening exercises, badminton, dance, and taekwondo. They generally had positive attitudes toward exercise, and more than 63% of participants intended to exercise the following month. The four most prevalent perceived barriers to exercise were lack of time, poor health, reluctance to sweat, and lack of exercise skills. Conclusions: While most childhood cancer survivors did not meet the PA recommendation, most of them agreed that exercise was beneficial, and they intended to participate in exercise. Exercise and PA programs should be tailored to the personal health and preferences of childhood cancer survivors to increase PA and exercise participation in this group.
Wind Intensity and Power Density in the Brazilian Dry Tropical Forests (Caatinga)
Authors: Silva Madson Tavares , de Sousa Welinagila Grangeiro , Cavalcanti Enilson Palmeira , Silva Vicente de Paulo Rodrigues da , Serrão Edivaldo Afonso de Oliveira 
Published: June 2021

Abstract
Wind speed has been widely used for energy purposes. Therefore, studies focused on its knowledge are extremely relevant to better benefit from this resource. The aim of this study is to analyze wind behavior and estimate wind power density (WPD) in the interior of northeastern Brazil, a region with predominance of the semi-arid climate, based on the data made available by the automatic station installed at the Experimental Farm in the municipality of São João do Cariri-PB, which come from the SONDA project and refer to the year 2007. Descriptive analysis techniques were used to identify the periods in which the wind behavior is more favorable to wind harnessing. From the results obtained, there was a predominance of southeast in the wind direction component. However, the values of both the observed wind speed (2, 25 and 50 m) and the wind speed estimated for the levels of 100 and 150 m, as well as the estimates of power density (50, 100 and 150 m) showed that the lowest records are present mainly in the first hours of the day, as well as in the first half of the year, while the highest values occur from 10 a.m. extending to the beginning of the night and prevail in the last six months of the year. These determinations denoted higher values of wind power density available for the second half of the year (mainly from August to December).
Olive Landscape Affects Bactrocera Oleae Abundance, Movement and Damage
Authors: Ortega Marta Quero , Moreno Natalia , Fernández Cristina E. , Pascual Susana 
Published: June 2021

Abstract
The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is a key pest of olive groves. Because of its economic importance and problems associated with chemical control, new approaches to reduce the damage caused by this pest and a deeper knowledge of the biology of the insect and the relationship of landscape structure to different biological parameters are needed. B. oleae can fly long distances and its ability to move within the landscape can determine the damage caused to olive groves. This work evaluates the effect of landscape structure on olive fruit fly abundance, movements and damage at three times of year—spring, early autumn and late autumn—in central Spain. This area is less dominated by olive groves than southern Spain, where the relationship between olive grove area and B. oleae abundance is already known. A cost-distance analysis is used to evaluate the landscape effect on the movement of the fly along the crop cycle. The olive grove area is the landscape composition factor with the greatest effect on the parameters studied, with a decrease in B. oleae abundance in a more complex landscape during spring and early autumn. The cost-distance analysis shows that the olive fruit fly moves mainly in spring, and amongst olive groves. There is no evidence that land uses other than olive groves serve as a summer refuge for B.oleae in the studied landscape context. Olive grove area and land use diversity index had significant effects on olive damage in more than one year.
Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA
Authors: Ranjbary Ali Ghorbani , Saleh Golnaz Karbalaei , Azimi Mohammadreza 
Published: June 2021

Abstract
Today, research into nanoparticles for diagnostic and therapeutic use in cancer is on the rise. In the present study, the effect of superparamagnetic iron oxide nanoparticles on the formation of apoptosis in HT-29 cells is investigated.In this study, we investigated the mechanisms of apoptosis induced by superparamagnetic iron oxide nanoparticles after MTT assay and determining the appropriate dose of 2.5 µg / mL to induce apoptosis in HT-29 cells.Superparamagnetic iron oxide nanoparticles increased the levels of ROS, Ca2 +, and DNA damage in HT-29 cells. Also, at the level of protein and mRNA, they increased the expression of Caspes 3 and 9 and significantly decreased Bcl-2 compared to the control group (P <0.0001).Fe3O4 causes apoptosis in cancer cells by increasing the level of ROS and intracellular calcium, followed by increasing the expression of caspases 3 and 9 and decreasing the expression of Bcl-2, as well as direct DNA damage.
Order Reduction based on Coulomb’s and Franklin’s laws Algorithm
Authors: Kumar Ram , Sikander Afzal 
Published: June 2021

Abstract
The Coulomb and Franklin laws (CFL) algorithm is used to construct a lower order model of higher-order continuous time linear time-invariant (LTI) systems in this study. CFL is quite easy to implement in obtaining reduced order model of large scale system in control engineering problem as it employs the combined effect of Coulomb’s and Franklin’s laws to find the best values in search space. The unknown coefficients are obtained using the CFLA methodology, which minimises the integral square error (ISE) between the original and proposed ROMs. To achieve the reduced order model, five practical systems of different orders are considered. Finally, multiple performance indicators such as the ISE, integral of absolute error (IAE), and integral of time multiplied by absolute error were calculated to determine the efficacy of the proposed methodology. The simulation results were compared to previously published well-known research.
Comprehensive Analysis of Expression, Prognosis and Immune Infiltrates for Kinesin Superfamily Members in Human Pancreatic Adenocarcinoma
Authors: Chen Zehao , Wang Jie , Xu Jian , Zhu Wenjie , Jiang Jianxin 
Published: June 2021

Abstract
BackgroundKinesin superfamily (KIFs) has a long-reported significant influence on the initiation, development, and progress of pancreatic adenocarcinoma (PAAD). However, the expression level of different KIFs in PAAD and its relationship with the prognosis and immune infiltration in patients with PAAD have not been fully elucidated.MethodsComprehensive bioinformatics analyses were done using data from UCSC XENA data hubs, TCGA database，Oncomine databases, GEO datasets, GEPIA, GTEx, The human protein atlas, Kaplan Meier plotter, cBioPortal, STRING and KEGG database. Then, the relationship between KIFs expression and tumor immune infiltrates was studied by using the TIMER database. ResultsA total of 24 differentially expressed KIFs at transcriptional levels were identified between tumor tissues and normal tissues with 1 (KIF1A) downregulated and 23 (KIF2A, KIF2C, KIF3A, KIF3B, KIF3C, KIF4A, KIF5B, KIF7, KIF9, KIF10, KIF11, KIF13A, KIF13B, KIF15, KIF16B, KIF18B, KIF20A, KIF20B, KIF21B, KIF22, KIF23, KIF26B, KIFC1) overexpressed by GEPIA. The protein expression level of KIF3A, KIF3B, KIF4A, KIF5B, KIF7, KIF9, CENPE, KIF11, KIF13A, KIF13B, KIF15, KIF16B, KIF18B, KIF20A, KIF20B, KIF21B, KIF22, KIF23, KIF26B, KIFC1 are higher in PAAD tissues than in the adjacent tissues, which is almost the same as the results of KIFs at transcriptional levels in PAAD. In addition, the expression levels of KIF1A, KIF1C, KIF3A, KIF5A, KIF5C, KIF6, KIF9, KIF13B, KIF19A, KIF21A, KIF24, KIF26B, KIFC1 are significantly correlated with the tumor stage of PAAD patients. As for prognosis value, the abnormal expression of KIF2C, KIF4A, KIF11, KIF15, KIF18B, KIF20A, KIF20B, KIF21B and KIF23 in PAAD patients is significantly related to the worse overall survival (OS) and relapse-free survival (RFS), and based on this, a 5-KIFs-based (KIF15, KIF20A, KIF20B, KIF21B, KIF23) risk score was generated by LASSO regression with a nomogram validated an accurate predictive efficacy. GO and KEGG enrichments revealed functions and pathways affected in PAAD. We also found that the expression of the 5-KIFs was significantly correlated with immune infiltrates, including B cells, CD8+ T cells, CD4+T cells, neutrophils, macrophages, and dendritic cells.ConclusionOur study may provide new insights into the choice of prognostic biomarkers and immunotherapy targets in PAAD patients.
Combinatorial Bioinformatics Analysis Reveals Novel Biomarkers for Improved Ovarian Cancer Prognosis
Authors: Zhang Xiaoyi , Zhu Shihong , Peng Min , Ma Hongbo 
Published: June 2021

Abstract
Background: Given the known lethality of highly frequent ovarian cancer (OC) among females, it is imperative to investigate potential biomarkers of prognostic and therapeutic significance. The objective of this study was to identify significant differentially expressed genes (DEGs) with poor prognosis and to explore their underlying mechanisms. Methods: We acquired three microarray datasets (GSE14407, GSE36668 and GSE18520), available from the public database GEO. We compared a total of 72 cancerous and 26 normal samples originating from ovarian tissues. GEO2R and Venn diagram tools were used to obtain DEGs, followed by the gene ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) analysis via Database for Annotation, Visualization and Integrated Discovery (DAVID). Subsequently, protein-protein interaction (PPI) network was constructed and visualized in Cytoscape. Results: Among three analyzed datasets, a total of 232 DEGs were common. The upregulated 108 genes were significantly enriched in the cell adhesion, cellular response to interleukin-1, positive regulation of transcription from DNA/RNA, and transcription, extracellular matrix/region, anchored membrane component, cell junction and golgi membrane, sequence-specific DNA binding, transcription factor activity, RNA polymerase II regulatory region, and DNA binding. The PPI network analysis via MCODE plug-in revealed a total of 14 upregulated genes. Kaplan-Meier plotter analysis revealed that 9 genes were associated with significantly worse survival among OC patients while 4 genes exhibited no significant effect. Gene Expression Profiling Interactive Analysis (GEPIA) showed that 13 DEGs had significantly higher expression in the ovarian cancerous tissues compared to the normal ones. Repeated KEGG analysis showed that 11 genes (CDC6, CCNE1, BUB1B, CCNB2, BUB1, SFN, TTK, CDC20, PTTG1, CDK1 and CDKN2A)) were mainly associated with cell cycle while 2 genes (SFN and RRM2) were related to p53 signaling pathway. Conclusion: Our findings identify potential upregulated DEGs of prognostic value among OC patients. This will facilitate to understand the underlying OC mechanisms and to implement targeted therapeutic measures.
Risk Factor Analysis of Clinical Outcomes of Total Aortic Arch Replacement and Frozen Elephant Trunk With Aortic Balloon Occlusion
Authors: Wang Luchen , Li Yunfeng , Dun Yaojun , Sun Xiaogang 
Published: June 2021

Abstract
Background: Total aortic arch replacement (TAR) with frozen elephant trunk (FET) requires hypothermic circulatory arrest (HCA) for 20 minutes, which increases the surgical risk. We invented an aortic balloon occlusion technique that requires 5 minutes of HCA on average to perform TAR with FET and investigated the possible merit of this new method in this study. Methods: This retrospective study included consecutive patients who underwent TAR and FET (consisting of 130 cases of aortic balloon occlusion group and 230 cases of conventional group) in Fuwai Hospital between August 2017 and February 2019. In addition to the postoperative complications, the alterations of blood routine tests, alanine transaminase (ALT) and aspartate transaminase (AST) during the in-hospital stay were also recorded. Results: The 30-day mortality rates were similar between the aortic balloon occlusion group (4.6%) and the conventional group (7.8%, P = 0.241). Multivariate analysis showed aortic balloon occlusion reduced postoperative acute kidney injury (23.1% vs 35.7%, P = 0.013) and hepatic injury (12.3% vs 27.8%, P = 0.001), and maintained similar cost to patients (25.5 vs 24.9 kUSD, P = 0.298). We also found that AST was high during intensive care unit (ICU) stay and recovered to normal before discharge, while ALT was not as high as AST in ICU but showed a rising tendency before discharge. The platelet count showed a rising tendency on postoperative day 3 and may exceed the preoperative value before discharge. Conclusions: The aortic balloon occlusion achieved the surgical goal of TAR with FET with an improved recovery process during the in-hospital stay.
Pseudoaneurysm With A Fistula To The Right Ventricle Late After Surgical Repair of Type A Aortic Dissection in A Patient with Systemic Lupus Erythematosus: A Case Report
Authors: Shimada Akie , Yamamoto Taira , Endo Daisuke , Nishida Kousuke , Matsushita Satoshi , Asai Tohru , Amano Atsushi 
Published: June 2021

Abstract
Background: Pseudoaneurysm with a shunt to the right ventricle after aortic repair for acute aortic dissection is an extremely rare and life-threatening condition. Surgical treatment is unavoidable, but surgery is complicated, and there are some pitfalls. Herein, we explain the reoperation procedure performed for a patient at high surgical risk by clarifying the shunt site using multi-modality imaging before surgery.Case presentation: A 69-year-old woman with a history of systemic lupus erythematosus (SLE) and Sjogren’s syndrome presented with a pseudoaneurysm 1 year after emergency surgery for acute type A aortic dissection. Eight years after the first operation, she experienced sudden chest pain and presented to the emergency department. Her dyspnea also worsened; therefore, echocardiography and three-dimensional computed tomography (3DCT) were performed, which revealed a pseudoaneurysm and a shunt to the right ventricle. The medical team attempted to close the shunt with a percutaneous catheter but was unsuccessful, and she was referred to our department for surgical treatment.The pseudoaneurysm originating from the proximal side of the aorta was large with a diameter of 55 mm, and echocardiography-gated 3DCT visualized the shunt from the pseudoaneurysm to the right ventricle. First, extracorporeal circulation was initiated, and a re-sternotomy was performed. We could not insert the left ventricular venting tube from the right side because of the size of the pseudoaneurysm; instead, the tube was inserted from the left atrial appendage. We found a half-circumferential disengaged anastomosis around the proximal anastomosis, which formed the large pseudoaneurysm leading to a fistula in the right ventricle. We closed the fistula and performed a Bentall operation.The patient had a good postoperative course and was discharged on postoperative day 21. She continued treatment for SLE and Sjogren’s syndrome and her inflammatory reaction improved.Conclusion: We performed a Bentall operation and closure of a fistula with a re-sternotomy in a patient with type A aortic dissection with systemic lupus erythematosus and Sjogren’s syndrome. Multimodal imaging is essential in defining the pseudoaneurysm and the fistula surrounding the anatomy while ensuring their resolution and guiding the approach for operation.
Association Between Cigarette Smoking Status And Voting Intentions: Cross Sectional Surveys In England 2015-2020
Authors: Cox Sharon , Brown Jamie , McQuire Cheryl , de Vocht Frank , Beard Emma , West Robert , Shahab Lion 
Published: June 2021

Abstract
Background and aims: Cigarette smoking takes place within a cultural and social context. Political views and practices are an important part of that context. To gain a better understanding of smoking, it may be helpful to understand its association with voting patterns as an expression of the political views and practices of the population who smoke. This study aimed to assess the association between cigarette smoking and voting intentions and to examine how far any association can be explained by sociodemographic factors and alcohol use.Methods: Pooled monthly representative repeat cross-sectional household surveys of adults (16+) in England (N = 55,482) between 2015-2020 were used to assess the association between cigarette smoking status and voting intentions, and whether this was accounted for by age, occupational grade, gender, region and alcohol use. Voting intention was measured by asking ‘How would you vote if there were a General Election tomorrow?’ Respondents chose from a list of the major English political parties or indicated their intention not to vote.Results: In adjusted multinomial regression, compared with intending to vote Conservative (majority party of government during the period), being undecided (aOR1.22 [1.13-1.33] <0.001), intending to vote Labour (aOR1.27 [1.16-1.36] <0.001), to vote “Other” (aOR1.54 [1.37-1.72] <0.001), or not to vote (aOR1.93 [1.77-2.11] <0.001) was associated with higher odds of current relative to never smoking rates. Intending to vote for the Liberal Democrats was associated with a significant lower odds of current smoking prevalence (aOR0.80 [0.70-0.91] <0.001) compared with intending to vote Conservative.Conclusions: Controlling for a range of other factors, current as compared with never-smokers appear more likely to intend not to vote, to be undecided, to vote for Labour or a non-mainstream party, and less likely to vote for the Liberal Democrats, compared with the Conservative party.