bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of platelet-rich and platelet-poor plasma on 3D bone-to-implant contact: a preclinical micro-CT study
Authors: Song Dandan , Shujaat Sohaib , Huang Yan , Van Dessel Jeroen , Politis Constantinus , Lambrichts Ivo , Jacobs Reinhilde 
Published: February 2021

Abstract
Background
Bone-to-implant contact ratio (BIC%) plays a critical role in secondary stability of osseointegrated dental implants. The aim of this study was to identify the correlation of 2D/3D micro-CT images with histology as a gold standard for evaluating BIC% and to investigate the influence of the platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on 3D BIC% following delayed implant placement with delayed loading (DIP+DL).

Methods
Nine beagle dogs were recruited. Following bilateral extraction of mandibular 3rd premolar, 4th premolar, and 1st molar, 54 screw-type titanium implants were inserted and randomly divided into one control and two test groups based on a split-mouth design. The control group involved DIP+DL (n = 18) and both test groups included DIP+DL with local application of PRP (n = 18) and PPP (n = 18). A BIC analysis was performed utilizing 2D histomorphometry and 2D/3D micro-CT. Following identification of correlation between histology and 2D/3D micro-CT images, a 3D micro-CT assessment of the 3D BIC% at three follow-up time-points (1, 3, and 6 months) was carried out for observing the influence of PRP and PPP on BIC.

Results
The 2D micro-CT BIC% values revealed a strong positive correlation with histology (r = 0.98, p < 0.001) and a moderate correlation existed with 3D micro-CT (r = 0. 67, p = 0.005). BIC levels at 1 month and combined influence of PPP and PRP irrespective of time-points revealed significantly higher 3D BIC% compared to the control. However, a reduction in 3D BIC% was observed at the 3rd and 6th month. No significant difference was observed between both PRP and PPP.

Conclusions
Both 2D and 3D micro-CT demonstrated a potential to be utilized as a complimentary method for assessing BIC compared to the histological gold standard. Overall, both PRP and PPP significantly facilitated bone healing and osseointegration with a higher 3D BIC at follow-up. However, their influence was reduced as the observation period was increased.
Simulated learning in rural community environment: pushing the boundary
Authors: Akber Burhanuddin Ali , Rajani Mehak Ismail , Khalid Farah , Docherty Charles 
Published: February 2021

AbstractExperiential learning through simulation can play a very significant role, not only in hospital settings but also in community contexts (Lubbers and Rossman, Nurse Educ. Today 48:140-144; Wheeler and McNelis, Nurs. Educ. Perspect 35:259-261). This paper discusses the concept of creating a novel simulated village set-up within a modern simulation center, to effectively deliver contemporary learning outcomes. It also highlights the challenges and risks of developing a simulated village set-up and strategies to counteract them. Furthermore, it describes the role of simulation specialists as innovators and explicates the gamut of expertise in education, management, and technologies that are required to deliver excellence in simulation-based education.
Hyperferritinemia in children hospitalized with scrub typhus
Authors: Williams Vijai , Menon Nisha , Bhatia Prateek , Biswal Manisha , Sreedharanunni Sreejesh , Jayashree Muralidharan , Nallasamy Karthi 
Published: February 2021

Abstract
Background
Hyperferritinemia is increasingly associated with mortality in sepsis. Studies estimating the prevalence of hyperferritinemia in pediatric scrub typhus are limited.

Methods
This was a secondary analysis of a prospective observational study (FERRIS) from a tertiary care teaching hospital in North India where 72 children with confirmed scrub typhus, 4 (5.5%) PCR positive, 55 (76.4%)-IgM ELISA positive, and 13 (18.1%)-both PCR and ELISA positive, were analyzed. Serum ferritin was measured in 62 children to identify the prevalence of hyperferritinemia and determine its association with mortality.

Results
Hyperferritinemia (> 500 μg/L) was seen in 72.6% [n = 45] children; 26 (41.9%) were mild (500–2000 μg/L), 13 (21%) were moderate (2000–10,000 μg/L), and 6 (9.7%) were severe (> 10,000 μg/L). Early presentation to hospital (≤ 7 days of febrile illness) had more survivors than late presentation (> 7 days). Non-survivors had significantly higher PRISM III, PELOD-2, hyperlactatemia, hypoalbuminemia, organ dysfunction, need for mechanical ventilation, and need of RRT. Ferritin had poor sensitivity and specificity in predicting survival with AUC of 0.56. Organ dysfunction and risk scores as PRISM III, PELOD 2, and VIS at admission were better predictors with AUC (95% CI) of 0.72 (0.56, 0.89), 0.77 (0.63, 0.92), and 0.90 (0.78, 1.0) respectively.

Conclusions
Hyperferritinemia is common in scrub typhus but it did not predict survival. Organ dysfunction and risk scores were better predictors of mortality than ferritin.
When more is more: redundant modifiers can facilitate visual search
Authors: Rehrig Gwendolyn , Cullimore Reese A. , Henderson John M. , Ferreira Fernanda 
Published: February 2021

Abstract
According to the Gricean Maxim of Quantity, speakers provide the amount of information listeners require to correctly interpret an utterance, and no more (Grice in Logic and conversation, 1975). However, speakers do tend to violate the Maxim of Quantity often, especially when the redundant information improves reference precision (Degen et al. in Psychol Rev 127(4):591–621, 2020). Redundant (non-contrastive) information may facilitate real-world search if it narrows the spatial scope under consideration, or improves target template specificity. The current study investigated whether non-contrastive modifiers that improve reference precision facilitate visual search in real-world scenes. In two visual search experiments, we compared search performance when perceptually relevant, but non-contrastive modifiers were included in the search instruction. Participants (NExp. 1 = 48, NExp. 2 = 48) searched for a unique target object following a search instruction that contained either no modifier, a location modifier (Experiment 1: on the top left, Experiment 2: on the shelf), or a color modifier (the black lamp). In Experiment 1 only, the target was located faster when the verbal instruction included either modifier, and there was an overall benefit of color modifiers in a combined analysis for scenes and conditions common to both experiments. The results suggest that violations of the Maxim of Quantity can facilitate search when the violations include task-relevant information that either augments the target template or constrains the search space, and when at least one modifier provides a highly reliable cue. Consistent with Degen et al. (2020), we conclude that listeners benefit from non-contrastive information that improves reference precision, and engage in rational reference comprehension.

Significance statement
This study investigated whether providing more information than someone needs to find an object in a photograph helps them to find that object more easily, even though it means they need to interpret a more complicated sentence. Before searching a scene, participants were either given information about where the object would be located in the scene, what color the object was, or were only told what object to search for. The results showed that providing additional information helped participants locate an object in an image more easily only when at least one piece of information communicated what part of the scene the object was in, which suggests that more information can be beneficial as long as that information is specific and helps the recipient achieve a goal. We conclude that people will pay attention to redundant information when it supports their task. In practice, our results suggest that instructions in other contexts (e.g., real-world navigation, using a smartphone app, prescription instructions, etc.) can benefit from the inclusion of what appears to be redundant information.
Assessment of China’s contributions to the Regional Network for Asian Schistosomiasis and Other Helminth Zoonoses: a questionnaire survey
Authors: Li Hong-Mei , Qian Ying-Jun , Yang Kun , Ding Wei , Huang Lu-Lu , Ma Xue-Jiao , Duan Lei , Wang Duo-Quan , Guan Ya-Yi , Xiao Ning , Zhou Xiao-Nong 
Published: February 2021

Abstract
Background
The Regional Network for Asian Schistosomiasis and Other Helminth Zoonoses (RNAS+) was established in 1998, which has developed close partnerships with Asian countries endemic for schistosomiasis and other helminthiasis in Asia. RNAS+ has provided an ideal regional platform for policy-makers, practitioners and researchers on the prevention, control and research of parasitic diseases in Asian countries. China, one of the initiating countries, has provided significant technical and financial support to the regional network. However, its roles and contributions have not been explored so far. The purpose of this study was to assess China's contributions on the supporting of RNAS+ development.

Methods
An assessment research framework was developed to evaluate China’s contributions to RNAS+ in four aspects, including capacity building, funding support, coordination, and cooperation. An anonymous web-based questionnaire was designed to acquire respondents’ basic information, and information on China’s contributions, challenges and recommendations for RNAS+development. Each participant scored from 0 to 10 to assess China’s contribution: “0” represents no contribution, and “10” represents 100% contribution. Participants who included their e-mail address in the 2017–2019 RNAS+ annual workshops were invited to participate in the assessment.

Results
Of 71 participants enrolled, 41 responded to the survey. 37 (37/41, 90.24%) of them were from RNAS+ member countries, while the other 4 (4/41, 9.76%) were international observers. Most of the respondents (38/41, 92.68%) were familiar with RNAS+. Respondents reported that China’s contributions mainly focused on improving capacity building, providing funding support, coordination responsibility, and joint application of cooperation programs on RNAS+ development. The average scores of China’s contributions in the above four fields were 8.92, 8.64, 8.75, and 8.67, respectively, with an overall assessment score of 8.81 (10 for a maximum score). The challenge of RNAS+ included the lack of sustainable funding, skills, etc. and most participants expressed their continual need of China’s support.

Conclusions
This survey showed that China has played an important role in the development of RNAS+ since its establishment. This network-type organization for disease control and research can yet be regarded as a great potential pattern for China to enhance regional cooperation. These findings can be used to promote future cooperation between China and other RNAS+ member countries.
Validity of neuron-specific enolase as a prognostic tool in acute ischemic stroke in adults at Suez Canal University Hospital
Authors: Shash Mohammed H. , Abdelrazek Reda , Abdelgeleel Nashwa M. , Ahmed Rasha M. , El-baih Adel H. 
Published: February 2021

Abstract
Background
Biological markers of acute nerve cell damage can assist in the outcome of acute ischemic stroke, such as neuron-specific enolase (NSE) that have been tested for association with initial severity of stroke, extent of infarction, and functional outcome.

Objective
To determine short-term prognostic value of the biochemical marker neuron-specific enolase (NSE) in acute ischemic stroke.

Methods
A cohort study carried out on 37 patients with acute ischemic stroke. Data were gathered in a prepared data sheet. Initial serum NSE level was measured to the patients in the Emergency department within 6 h of the onset of stroke and another measurement after 48 h. National Institute of Health Stroke Scale (NIHSS) was held to the patients at presentation and after 28 days of stroke to determine short-term morbidity and mortality.

Results
Out of the 37 patients, 31 patients survived (no-death group) and 6 patients died (death group). The mean serum level of neuron-specific enolase at presentation and after 48 h was significantly higher in the death group than in the no-death group. There was a statistically significant positive correlation between neuron-specific enolase (NSE) serum level and clinical severity of stroke (NIHSS) among the patients at presentation (r = 0.737, p = 0.000).

Conclusion
Neuron-specific enolase (NSE) can be applied as single independent marker for prediction of mortality and short-term morbidity in ischemic stroke patients.
Genetic variability and association between oil and economic traits for some new Egyptian cotton genotypes
Authors: Eldessouky Sara E. I. , El-Fesheikawy A. B. A. , Baker K. M. A. 
Published: February 2021

Abstract
Background
Cotton breeding programs mainly focus on improving cotton fibers, but less attention has been paid by breeders to cottonseed oil improvement. Breeding cottonseed for oil content has mainly depended on phenotypic information used to select varieties with high seed oil content. The purpose of this study was to evaluate some cotton genotypes regarding their oil content and other characters related to fiber yield, in order to select genotypes with high oil content and acceptable levels of the other characters. Seventeen different genotypes of cotton were used in this study. A two-year experiment was carried out at Sids Agricultural Research Station, Beni Suef, Egypt, during 2017 and 2018, using a randomized complete block design with six replications. Seed cotton yield, lint cotton yield, boll weight, seed index, embryo index, oil % and embryo oil index characters were recorded.

Results
The analysis of variance results showed highly significant differences among the genotypes for all the studied traits except boll weight in season 2017. The overall cotton embryo oil % ranged from 33.40 to 40.28 among the genotypes, while the overall cotton embryo oil index ranged from 1.79 to 2.47. Maximum cotton embryo oil % was noted by the genotypes 15 [{(G83 × G80) × G89} × Australian] and 8 [(G 91 × G90) × (G 85 × G 83)], while maximum cotton embryo oil index was noted by the genotype 13 [(G 91 × G 90) × G80]. The results showed positive correlations between seed cotton yield and lint cotton yield, seed index and embryo index, seed index and embryo index oil %, embryo index and embryo index oil %, and oil % and embryo index oil %.

Conclusions
It could be concluded that using seed or embryo oil percentages as criteria for differentiating genotypes according to their oil content is misleading and the best efficient measure is using the seed or embryo oil indices because they depend on weight basis. Also, Genotypes 13, 15 and 8 were the best genotypes regarding oil % and they could be used in breeding programs for cotton oil improvement.
Population dynamics of Fall Army Worm [(Spodoptera frugiperda) J.E. Smith] (Lepidoptera: Nuctuidae) in maize-cassava intercrop using pheromone traps in Niger Delta Region
Authors: Nwanze J. A. C. , Bob-Manuel R. B. , Zakka U. , Kingsley-Umana E. B. 
Published: February 2021

AbstractBackgroundThis study was conducted to generate baseline information on population dynamics of Fall Army Worm (FAW) in cassava-maize intercrop for management technique. Maize (Zea mays) is Africa’s most staple food crop with pest complex as major constraints to its production. The study was carried out at the Abuja Campus of the University of Port Harcourt, Faculty of Agriculture Teaching and Research Farm. A plot size of 3298 m2was cleared and ploughed; afterward, thirty six (36) ridges were made for the planting. Three varieties of maize grains (a hybrid Oba Super 98, white and yellow locals) were used for the study. Two cropping patterns (Sole maize and Cassava-Maize.-Intercrop) as main factor with a total of 18 sole and 18 intercrop plots and pheromone trap heights (at 1 m and 1.5 m) as sub-factor were used. The traps were mounted 18 days after planting and insect collection commenced at dawn the following day. FAW data in each trap were collected daily between 06.00 and 07.00 h. Maize cobs, fresh and dry weights, numbers of FAW exit holes, tunnels and tunnel lengths were recorded for both cropping patterns in each maize variety. Data were subjected to two-way analysis of variance.ResultsThe results show higher mean value of FAW count in pheromone trap height placed at 1.5 m, and Oba super 98 maize variety intercropped with cassava had higher FAW count. There were significantly higher (P < 0.05) FAW exit holes in maize with pheromone trap height placed at 1.5 m, and maize-cassava intercrops had higher mean values of FAW exit holes. Number of tunnels and tunnel lengths (cm) due to FAW infestation were higher in maize varieties where pheromone traps were placed at 1.5 m.ConclusionIntercropping maize with cassava may suggest increase in FAW bionomics and the presence of abundant host which might increase a spike in its peak period of infestation. The presence of cassava in maize-cassava cropping pattern encourages feeding and/or oviposition of FAW on maize plant; therefore, an alternative cropping pattern should be encouraged in the region.
Using Google Trends to assess the impact of Global Public Health Days on online health information-seeking behaviour in Arabian Peninsula
Authors: Ajbar Aymane , Shepherd Thomas A. , Robinson Michelle , Mallen Christian D. , Prior James A. 
Published: February 2021

Abstract
Background
Global Public Health Days (GPHD) are public health interventions which serve to improve public awareness of specific health conditions. Google Trends is a publicly available tool that allows the user to view the popularity of a searched keyword during a specified time period and across a predetermined region. Our objective was to use Google Trends to assess the impact of four GPHD (World Heart Day, World Mental Health Day, World Diabetes Day and World Hypertension Day) on online health information-seeking behaviour (OHISB), 4 weeks before and a week after the GPHD, across six countries of the Arabian Peninsula (Bahrain, Kuwait, Oman, Qatar, Saudi Arabia and United Arab Emirates).

Methods
Relative Search Volume (RSV) was extracted for the aforementioned countries from 28 days before the GPHD and 7 days afterwards. Statistical analysis, undertaken using joinpoint regression software, showed that GPHD have significant changes for Saudi Arabia (Diabetes, Mental Health and Heart day) and UAE (Mental Health day) but were short-lived with a fall in RSV of up to 80% after peak interest.

Conclusion
GPHD appears to be effective in some countries while further research is needed to investigate the reason of its limitations.
The dysbiosis of ovine foot microbiome during the development and treatment of contagious ovine digital dermatitis
Authors: Duncan J. S. , Angell J. W. , Richards P. , Lenzi L. , Staton G. J. , Grove-White D. , Clegg S. , Oikonomou G. , Carter S. D. , Evans N. J. 
Published: February 2021

AbstractBackgroundContagious Ovine Digital Dermatitis (CODD) is an emerging and common infectious foot disease of sheep which causes severe welfare and economic problems for the sheep industry. The aetiology of the disease is not fully understood and control of the disease is problematic. The aim of this study was to investigate the polybacterial aetiopathogenesis of CODD and the effects of antibiotic treatment, in a longitudinal study of an experimentally induced disease outbreak using a 16S rRNA gene amplicon sequencing approach.ResultsCODD was induced in 15/30 experimental sheep. During the development of CODD three distinct phenotypic lesion stages were observed. These were an initial interdigital dermatitis (ID) lesion, followed by a footrot (FR) lesion, then finally a CODD lesion. Distinct microbiota were observed for each lesion in terms of microbial diversity, clustering and composition.Porphyromonadaceae, Family XI, VeillonellaceaeandFusobacteriaceaewere significantly associated with the diseased feet.VeillonellaceaeandFusobacteriaceaewere most associated with the earlier stages of ID and footrot rather than CODD. Following antibiotic treatment of the sheep, the foot microbiota showed a strong tendency to return to the composition of the healthy state. The microbiota composition of CODD lesions collected by swab and biopsy methods were different. In particular, theSpirochaetaceaefamily were more abundant in samples collected by the biopsy method, suggesting that these bacteria are present in deeper tissues of the diseased foot.ConclusionIn this study, CODD presented as part of a spectrum of poly-bacterial foot disease strongly associated with bacterial familiesPorphyromonadaceae, Family XI(a family in Clostridiales also known as Clostridium cluster XI), VeillonellaceaeandFusobacteriaceaewhich are predominately Gram-negative anaerobes. Following antibiotic treatment, the microbiome showed a strong tendency to return to the composition of the healthy state. The composition of the healthy foot microbiome does not influence susceptibility to CODD.Based on the data presented here and that CODD appears to be the severest end stage of sheep infectious foot disease lesions, better control of the initial ID and FR lesions would enable better control of CODD and enable better animal welfare.
Analysis of the androgen receptor (AR) gene in a cohort of Indonesian undermasculinized 46, XY DSD patients
Authors: Listyasari Nurin Aisyiyah , Juniarto Achmad Zulfa , Robevska Gorjana , Ayers Katie L. , Sinclair Andrew H. , Faradz Sultana M. H. 
Published: February 2021

Abstract
Background
Pathogenic variants in the androgen receptor (AR) gene located on chromosome Xq11-12, are known to cause varying degrees of undermasculinization in 46, XY individuals. The aim of this study was to investigate the frequency of pathogenic variants in the AR gene in a cohort of 46, XY undermasculinized individuals from Indonesia who were suspected of having androgen insensitivity syndrome (AIS). All patients with 46, XY DSD referred to our center between 1994 and 2019 were collected from our clinical database. All 46, XY DSD patients without a prior molecular diagnosis with an external masculinization score (EMS) ≤ 9 were included in this study. All exons and intron–exon boundaries of AR gene were analyzed using Sanger sequencing to identify pathogenic variants of the AR gene.

Results
A cohort of 75 undermasculinized patients were selected for the study. Direct Sanger sequencing of all eight exons of the AR gene led to a genetic diagnosis in 11 patients (14.67%). All of the variants identified (p.Arg841His; p.Ile604Asn; p.Val731Met; p.Pro672Ser; p.Gln739Arg; p.Ser302Glufs*3) have been previously reported in patients with AIS.

Conclusions
This is the first study in Indonesia that highlights the significance of molecular analysis in providing a definitive diagnosis of AIS for patients with 46, XY DSD undermasculinization. This is an uncommon finding in the Indonesian population presenting with 46, XY DSD undermasculinization. A genetic diagnosis allows optimal clinical management and genetic counseling for patients and their families. As 46, XY DSD can be caused by pathogenic variants in other genes involved in gonadal development and differentiation, further genetic analysis, such as whole exome sequencing, should be carried out on those patients that did not carry an AR variant.