bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of response to bDMARDs and tsDMARDs in psoriatic arthritis: a pilot study on the role of musculoskeletal ultrasound
Authors: Cozzi Giacomo , Scagnellato Laura , Lorenzin Mariagrazia , Collesei Antonio , Oliviero Francesca , Damasco Amelia , Cosma Chiara , Basso Daniela , Doria Andrea , Ramonda Roberta 
Published: December 2024

ObjectivesThis pilot study aimed to identify early predictors of drug retention in patients with clinically active peripheral psoriatic arthritis who initiated or switched to therapy with biologic and targeted synthetic disease-modifying antirheumatic drugs (bDMARDs and tsDMARDs).MethodsClinical and ultrasound assessments were conducted at baseline (t0) and subsequently at 1 (t1), 3 (t3), and 6 (t6) months. Ultrasound evaluations targeted joints/entheses according to PsASon-Score13 and the most clinically involved joint/enthesis/tendon or the two most clinically involved joints/entheses/tendons (MIJET and 2MIJET). After 6 months of follow-up, patients were divided into two groups based on drug retention, determined by the clinician's assessment of treatment efficacy (cResponder vs. non-cResponder). Main endpoints were ultrasound changes in MIJET, 2MIJET, and GUIS (Global US Inflammation Subscore) derived from PsASon-13.ResultsTwenty-nine patients were enrolled, 22 cResponders and 7 non-cResponders at t6. In the comparison between cResponders and non-cResponders, GUIS variation significantly differed in Δt6-t0, while MIJET and 2MIJET variations were significant as early as Δt3-t0 and confirmed in Δt6-t0. The ultrasound response of MIJET and 2MIJET was faster in cResponders treated with JAKi vs. those treated with TNFi and IL-17/12-23i, significant in Δt1-t0.ConclusionsUltrasound imaging of clinically involved joint sites may be a valuable early predictor of therapy response for predicting drug retention at 6 months in patients with psoriatic arthritis.
Overcoming tet(X)-harboring tigecycline resistance: a study on the efficacy of tigecycline-apramycin combinations
Authors: Liu Juan , Zheng Si-Lin , Wu Jing-Jing , Zheng Mei , Cai Da-Tong , Zhang Yan , Sun Jian , Liao Xiao-Ping , Yu Yang 
Published: December 2024

IntroductionThe emergence of the wide variety of novel tigecycline resistance tet(X) variants, including tet(X3), tet(X4), tet(X5), and tet(X6), has raised a serious threat to global public health and posed a significant challenge to the clinical treatment of multidrug-resistant bacterial infections.MethodsIn this study, we evaluated the synergism of tigecycline combining with other antibiotics as a means of overcoming the tet(X)-mediated resistance in Acinetobacter spp. Antibiotic synergistic efficacy was evaluated through in vitro chequerboard experiments, time-kill assays and dose–response curves. The in vivo synergistic effect of the combination was confirmed in a mouse model of thigh with neutrophilic granulocyte reduction. Additionally, combinations were tested for their ability to prevent high-level tigecycline-resistant mutants.ResultsWe found that the combinations of tigecycline with apramycin exhibited synergistic activity against tet(X)-harboring Acinetobacter spp. with FICI of 0.088. The MICTGC decreased more than 5 times in the presence of subinhibitory levels of apramycin. The combination showed in vitro synergism in time-kill assays and in vivo therapeutic effectiveness in the mouse thigh infection model.DiscussionThis study shed light on the synergism of tigecycline in combination with apramycin which offers a viable therapeutic alternative for infections caused by tet(X)-harboring Acinetobacter spp.
A human monoclonal antibody isolated from Japanese encephalitis virus vaccine-vaccinated volunteer neutralizing various flaviviruses
Authors: Chen Dong , Zhang Jiayi , Liu Yusha , Zhu Jiayang , Chen Jie , Ni Hongxia , Wen Jinsheng 
Published: December 2024

IntroductionJapanese encephalitis virus (JEV) and Zika virus (ZIKV) are prevalent in over 80 countries or territories worldwide, causing hundreds of thousands of cases annually. But currently there is a lack of specific antiviral agents and effective vaccines.MethodsIn the present study, to identify human neutralizing monoclonal antibody (mAb) against JEV or/and ZIKV, we isolated ZIKV-E protein-binding B cells from the peripheral venous blood of a healthy volunteer who had received the JEV live-attenuated vaccine and performed 10× Genomics transcriptome sequencing and BCR sequencing analysis, we then obtained the V region amino acid sequences of a novel mAb LZY3412. We expressed mAb LZY3412 and evaluated its ability to bind to E proteins of dengue virus, JEV and ZIKV, neutralize JEV and ZIKV infections in vitro, protect mice against lethal JEV or ZIKV attack. The epitopes on E proteins of JEV/ZIKV recognized by mAb LZY3412 were analyzed using molecular docking and constructing E protein mutants.ResultsOur results show that recombinant mAb LZY3412 has high-affinity with the E proteins of three viruses, with the kinetically derived binding affinity (KD) values of 440 and 482.5 nM against JEV-E protein and ZIKV-E protein, respectively; recombinant mAb LZY3412 can efficiently neutralize JEV and ZIKV infections in vitro, with the NT50 values of 19.9 ng/mL and 631 ng/mL, respectively; application of recombinant mAb LZY3412 can significantly improve the percentage survival and reduce the serum viral loads of neonatal mice infected with JEV or ZIKV. Finally, two amino acid residues (Ala399 and Gly400) located in EDIII of JEV-E protein were potentially recognized by LZY3412 whereas two amino acid residues (Met15 and Thr406) out of EDIII of ZIKV-E proteins were recognized by LZY3412.DiscussionConsidering mAb LZY3412 has neutralizing effects on various flaviviruses, this mAb is expected to be developed as therapeutic or preventive drug for these flaviviruses, and can also be used to guide the development of vaccines against these flaviviruses.
Clinical profile and molecular genetic analysis of alport syndrome in children: a single center experience
Authors: Ahmad Aqsa , Lijun Liang , Yan Zhang , Yan Ma , Shuai Zhao , Wangnan Du 
Published: December 2024

BackgroundAlport syndrome (AS) is a multifaceted condition that primarily affects the basement membranes of the kidneys, ears, and eyes. AS is considered the second most common cause of hereditary renal failure, exhibiting varied clinical manifestations across different lifespans. The aim of this study is to investigate the clinical features and genetic profile of AS and to elucidate the genotype-phenotype correlation of AS.MethodThe clinical and genetic data of ten children with AS treated at the General Hospital of Ningxia Medical University between January 2021 and May 2024 were retrospectively analyzed.ResultsTen children with AS, six male and four female patients, with a mean age of 9 years (ranging from 3 to 15 years) were reported. Hematuria was observed in all individuals, with six cases exhibiting microscopic hematuria and four cases exhibiting macroscopic hematuria. Furthermore, extra-renal manifestations were noted in five cases, encompassing ocular abnormalities (n = 2) and hearing impairment (n = 3). In total, eight cases displayed mutations in COL4A5 indicating XLAS, while two cases manifested mutations in COL4A4 indicating ADAS. Nine different variants were detected, with 3 mutations identified as novel. Two cases underwent histopathological analysis, revealing a thin basement membrane and mild to moderate mesangial proliferation. Three cases were lost to follow-up, while the remaining seven maintained regular visits to our hospital. As of August 1st, 2024, the median follow-up time was 30 (range 24–36) months, and the renal function of the children under observation remained within normal parameters.ConclusionIn this study, the most commonly observed mutation was glycine substitution. Additionally, patients exhibiting severe mutations showed an increased vulnerability to complications, including proteinuria, ocular lesions, and hearing impairment. Genetic testing emerged as a critical resource for diagnosing AS. Furthermore, early diagnosis is crucial for implementing an appropriate management plan and assessing the prognosis.
Biocompatible nanoparticles self-assembled by PEGylated polyphosphoesters for combination of photodynamic therapy and hypoxia-activated chemotherapy against breast cancer
Authors: Wang Fengyu , Zou Xiaojing , Sun Chunyang 
Published: December 2024

IntroductionAlthough photodynamic therapy (PDT) shows considerable potential for cancer treatment due to its precise spatial control and reduced toxicity, effectively eliminating residual cells under hypoxic conditions remains challenging because of the resistance conferred by these cells.MethodsHerein, we synthesize an amphiphilic PEGylated polyphosphoester and present a nanocarrier (NPCT) specifically designed for the codelivery of hydrophobic photosensitizer (chlorin e6, Ce6) and hypoxia-activated prodrugs (tirapazamine, TPZ). We investigate the antitumor effect of NPCT on both cellular and animal level.ResultsThe efficient encapsulation of Ce6 and TPZ by NPCT enables the prolonged blood circulation and improved tumor distribution of both agents. Upon internalization by tumoral cells, 660 nm laser irradiation activates Ce6, leading to the generation of reactive oxygen species (ROS) that effectively kill murine 4T1 breast cancer cells. Meanwhile, the PDT process consumes a large amount of oxygen to generate the hypoxic microenvironment that activates the liberated TPZ from NPCT. The resulting highly cytotoxic radicals specifically target and induce cytotoxicity in remaining hypoxic cancer cells. Compared to other groups, the combination of NPCT and 660 nm laser irradiation resulted in the most substantial tumor growth inhibition.DiscussionThis innovative approach provides new avenues for the development of advanced delivery systems based on polyphosphoesters and combination therapeutic strategies.
The effects of aerobic exercise on 24-hour mean blood glucose levels measured by continuous glucose monitoring in type 2 diabetes: a meta-analysis
Authors: Wang Chou , Tang Shaokai 
Published: December 2024

PurposeTo examine the effects of structured aerobic exercise on 24-hour mean blood glucose outcomes assessed by continuous glucose monitors in adults with type 2 diabetes.MethodsThe study established specific inclusion and exclusion criteria and conducted a comprehensive search across five databases, including PubMed, Web of Science, Embase, Cochrane Library, and EBSCOhost from the start year of each database’s coverage to 22 July 2024. The quality of the included studies was evaluated using the Cochrane Handbook 5.1 guidelines. Data analysis was performed using Review Manager 5.4 to determine effect sizes, conduct sensitivity analyses, assess potential biases, and perform subgroup analyses.ResultsA total of 1,034 articles were retrieved, and after 4 rounds of screening, 13 articles were finally selected for meta-analysis. The study included 626 participants (30% female; mean ± SD: age, 59.4 ± 6.4 years; BMI, 29.61 ± 2.24 kg/m2), including 330 in the experimental group and 296 in the control group. The results of the meta-analysis showed that aerobic exercise can improve the 24-hour mean blood glucose in patients with T2DM (d = −0.65, 95% CI: −0.75 to −0.55, p &lt; 0.05). Subgroup analysis showed that moderate-intensity and high-intensity aerobic exercise can improve the 24-hour mean blood glucose in patients with T2DM (d = −0.71, 95% CI: −0.81 to −0.60, p &lt; 0.05), (d = −0.60, 95% CI: −0.98 to −0.22, p &lt; 0.05). Also, 20–40 min and 40–60 min of aerobic exercise per session can improve the 24-hour average blood glucose in patients with T2DM (d = −0.75, 95% CI: −0.91 to −0.59, p &lt; 0.05), (d = −0.59, 95% CI: −0.71 to −0.46, p &lt; 0.05). Aerobic exercise can improve the 24-hour mean blood glucose in patients with T2DM who have a body mass index (BMI) between 29 and 30 kg/m2, as well as those with a BMI greater than 30 kg/m2 (d = −0.65, 95% CI: −0.94 to −0.36, p &lt; 0.05), (d = −0.76, 95% CI: −0.87 to −0.64, p &lt; 0.05).ConclusionAerobic exercise can improve the 24-hour mean blood glucose in patients with T2DM. Additionally, 20–60 min of aerobic exercise with moderate intensity, and high intensity can improve the 24-hour mean blood glucose in patients with T2DM who have a BMI greater than 29 kg/m2.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifer PROSPERO CRD42024590812
Fine mapping and identification of the bright green leaf gene BoBGL in Chinese kale (Brassica oleracea var. alboglabra)
Authors: Zhang Qi , Wang Chenchen , Song Jialu , Gao Liwei , Shen Wenjie , Liu Yi , Chen Daozong , Tan Chen 
Published: December 2024

IntroductionChinese kale (Brassica oleracea var. alboglabra), is an annual herb belonging to the Brassica genus of Cruciferae, and is one of the famous specialty vegetables of southern China. Some varieties show bright green leaf (BGL) traits and have better commerciality. However, the genes responsible for this trait remain unidentified.MethodsIn this study, gene mapping was measured by BSR-Seq and molecular marker analysis. Gene expression analysis was performed qRT-PCR. Cloning and sequence analysis of candidate genes were also performed.ResultsGenetic analysis revealed that the bright green leaf trait is a dominant trait governed by a single pair of genes. BSR-seq and molecular marker validation mapped the candidate interval to about 1.5 Mb on chromosome C8. After expanding the BC1 population and analyzing recombinant individuals, the interval was refined to approximately 102 kb on chromosome C8 (50,787,908- 50,890,279 bp). There are 24 genes in this region, and after annotation and expression analysis, BolC8t52930H (BoCER1.C8), associated with wax synthesis, emerged as a key candidate for BoBGL. We cloned this gene from both parents, revealing significant differences in their promoter regions. A co-segregation primer was subsequently developed and validated in a segregated population, with results consistent with expectations.DiscussionThe gene BoCER1.C8 is a potential candidate for controlling the bright green leaf trait in Chinese kale, and its function needs to be validated next. Mapping and cloning this gene is crucial to understanding wax synthesis regulation and developing new bright green leafy varieties of Chinese kale.
Can motor competence be influenced by the type of training interventions preschool children are exposed to? A randomized experimental study comparing sports games and psychomotricity activities
Authors: Guo Xiaodan , Li Chuangtao , Zhang Zhaoxiang , Silva Ana Filipa , Clemente Filipe Manuel 
Published: December 2024

IntroductionThis study aimed to compare the effects of structured sports games (SG) and psychomotricity activities (PCM) on the locomotor, stability, and manipulative motor competencies of preschool children.MethodsA randomized controlled trial was conducted over an 8-week period, involving two experimental groups (SG, n = 30 and PCM, n = 30) and one control group (CG, n = 28), with participants attending two intervention sessions per week. A total of 88 5-year-old children participated in the experiment (boys n = 48; girls n = 40). They were evaluated three times (at baseline, after 4 weeks, and after 8 weeks) using the Motor Competence Assessment test to measure their locomotor, stability, and manipulative motor competencies.ResultsThe scores were standardized to percentiles based on sex and age. However, significant differences were observed between groups post-intervention in the locomotor domain (p = 0.003; ηp2 = 0.128), with the SG showing significantly higher values compared to the CG (mean difference: 17.0%; p = 0.021; d = 0.783), and PCM (mean difference: 19.8%; p = 0.005; d = 0.947). Additionally, significant differences were found between groups post-intervention in the manipulative domain (p = 0.001; ηp2 = 0.142), with the SG showing significantly higher values compared to the CG (mean difference: 19.3%; p = 0.009; d = 0.845) and PCM (mean difference: 21.4%; p = 0.003; d = 0.998).DiscussionOur study highlights the significant benefits of increased practice in developing motor competence, particularly in children’s locomotor and manipulative skills. Additionally, at this age, fun, competition, and social interaction seem to play a crucial role, as the SG group demonstrated greater improvements compared to the PCM group.
The roles of psychological needs satisfaction and impulsivity to parent-child conflict and non-suicidal self-injury
Authors: Wei Chang , Liu Bao , Wang Yu , Wang Yaping , Xu Qian 
Published: December 2024

IntroductionNon-suicidal self-injury is a serious health problem among adolescents. However, the association between parent–child conflict and adolescent non-suicidal self-injury and its underlying mechanisms have not been studied sufficiently. Based on the interpersonal model of non-suicidal self-injury, we tested the relationship between parent–child conflict and adolescent non-suicidal self-injury. Furthermore, based on self-determination theory and the diathesis-stress model, we examined whether psychological needs satisfaction mediated the link between parent–child conflict and adolescent non-suicidal self-injury, and if impulsivity moderated this mediating effect.MethodsUsing cross-sectional design, we recruited 656 adolescents (Mage = 13.43; 47% female) from two junior high schools in the Hubei province of China.ResultsThe results indicated a positive association between parent–child conflict and adolescent non-suicidal self-injury. Psychological needs satisfaction mediated the relationship between parent–child conflict and adolescent non-suicidal self-injury. High impulsivity strengthened the indirect effect of parent–child conflict on adolescent non-suicidal self-injury. Specifically, high impulsivity strengthened the direct relationship between psychological needs satisfaction and adolescent non-suicidal self-injury and further strengthened the indirect association between parent–child conflict and adolescent non-suicidal self-injury.ConclusionsOur findings highlight the potential mechanisms underlining the relationship between parent–child conflict and adolescent non-suicidal self-injury. Our findings can inspire educational practitioners to focus on the interaction of family risk factors and individual risk factors when developing intervention programs for adolescent non-suicidal self-injury.
Projecting temperature-related dengue burden in the Philippines under various socioeconomic pathway scenarios
Authors: Seposo Xerxes , Valenzuela Sary , Apostol Geminn Louis C. , Wangkay Keith Alexius , Lao Percival Ethan , Enriquez Anna Beatrice 
Published: December 2024

IntroductionAs climate change advances, the looming threat of dengue fever, intricately tied to rising temperatures, intensifies, posing a substantial and enduring public health challenge in the Philippines. This study aims to investigate the historical and projected excess dengue disease burden attributable to temperature to help inform climate change policies, and guide resource allocation for strategic climate change and dengue disease interventions.MethodsThe study utilized established temperature-dengue risk functions to estimate the historical dengue burden attributable to increased temperatures. Future projections were derived using Coupled Model Intercomparison Project Phase 6 (CMIP6) Shared Socioeconomic Pathway (SSP) scenarios to estimate the excess dengue burden on a national scale. Current health burden estimates were calculated by charting the attributable fraction per epidemiological week against the exponential risk function.ResultsProjections indicate a substantial increase in temperature-related dengue incidence across all SSP climate scenarios by 2100. Between 2010-2019, 72.1% of reported dengue cases in the Philippines were attributable to temperature, demonstrating that temperature is a significant driver in dengue transmission. The highest attributable fractions were observed between the warm-dry season to early rainy season (Epi Weeks 15–25). Southern, periequatorial areas, particularly those undergoing rapid urbanization, had the highest temperature-related dengue incidence.DiscussionThe findings emphasize the critical interplay between climate change and socioeconomic factors in shaping future dengue risk. By incorporating future climate scenarios and provincial-level projections, this study provides valuable insights for policy planning, early warning systems, and public health programming. Strengthening health infrastructure, promoting sustainable urban development, and implementing effective vector control measures are crucial to mitigating the future dengue burden in the Philippines.
Physical activity and anxiety during the COVID-19 pandemic in Tanzania: insights for public health policy in low-income contexts
Authors: Ndabi Joyce Sifa , Simwanza Alfa , Chukwuorji JohnBosco C. , Tladi Dawn , Muomah Rosemary C. , Nwonyi Sampson K. , Tay Doris Akosua , Joachim Dale , Malete Leapetswe , Adamba Clement , Nyawornota Vida Korleki , Nyanynofio Oscar Nyanyo , Donkor Samuel Kofi , Ocansey Reginald 
Published: December 2024

BackgroundThe COVID-19 pandemic heightened anxiety levels globally, disproportionately affecting low-and middle-income countries (LMICs). Physical activity (PA) has shown potential to alleviate mental health challenges, including anxiety. This study explores the relationship between PA and anxiety among Tanzanian adults during the pandemic, examining whether self-reported health status moderates this relationship and identifying demographic variations.MethodsData from 213 adults were collected using the International Physical Activity Questionnaire-Short Form (IPAQ-SF) and Generalized Anxiety Disorder-7 (GAD-7) scale. Self-reported health was also reported.ResultsVigorous physical activity was significantly associated with lower anxiety, while moderate physical activity showed weaker effects. Walking and sedentary behavior were not significantly associated with anxiety. Health status did not moderate these relationships, but subgroup analyses indicated stronger effects of vigorous physical activity among men and younger adults.ConclusionThese findings highlight the relevance of PA, particularly vigorous and moderate physical activity, in supporting mental health in LMIC contexts such as Tanzania. The findings further underscore the importance of targeted, culturally relevant physical activity interventions in LMICs to mitigate anxiety and enhance mental health resilience.