bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra-articular injection of orthobiologics in patients undergoing high tibial osteotomy for knee osteoarthritis is safe and effective – a systematic review
Authors: Betzler Brjan Kaiji , Bin Muhammad Ridzwan Chew Aiman Haziq , Bin Abd Razak Hamid Rahmatullah 
Published: September 2021

AbstractPurposeTo qualitatively evaluate the current evidence reporting outcomes of intra-articular injection of orthobiologics in patients undergoing high tibial osteotomy (HTO) for osteoarthritis of the knee.MethodsA systematic search methodology of the PUBMED, EMBASE, and CINAHL databases was conducted in July 2021. The search workflow was in adherence to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The following inclusion criteria were adopted: clinical trials of any level of evidence, reporting outcomes following intra-articular injection of orthobiologics during high tibial osteotomy for knee osteoarthritis, with a minimum number of 10 patients treated. Duplicate data, studies on implanted orthobiologics and articles not written in English were excluded from this review.ResultsEight studies were included in this review, with a total of 585 patients. Outcomes were discussed based on the types of orthobiologics used: (i) Platelet-Rich Plasma (PRP), (ii) Bone Marrow Aspirate Concentrate (BMAC), and (iii) Injected Mesenchymal Stem Cells (MSCs). Two studies utilised PRP, 4 studies utilised BMAC and 4 studies utilised injected MSCs.. Three studies provided Level II evidence and five studies provided Level III evidence. Statistically significant improvements in outcomes were documented in multiple trials, with few patients experiencing adverse events.ConclusionIntra-articular injection of orthobiologics in patients undergoing HTO is safe and effective with good outcomes reported. Due to the lack of high-level evidence, further research is required before this can be considered standard of care.Level of evidenceIII
Biomechanical study of a newly developed continuous double knots technique compared with the 4-strand double-modified Kessler technique for flexor tendon repair
Authors: Wongsiri Sunton , Liawrungrueang Wongthawat 
Published: September 2021

Abstract
Purpose
In this study we compare the biomechanical properties of a novel suture technique that we developed called the continuous double knots technique for repairing flexor tendon injuries with the standard 4-strand double-modified Kessler technique.

Methods
This was an experimental study. Eighty porcine flexor digitorum profundus tendons were harvested and divided randomly into two groups of 40. The first group (N = 40) was repaired using the 4-strand double modified Kessler technique and the second group (N = 40) was repaired using our new continuous double knots technique. The two groups were randomly divided and the ultimate failure load (n = 20) and cyclic testing to failure (n = 20) were compared.

Results
The mean ultimate failure load was 25.90 ± 7.11 (N) and cyclic testing to failure 88 ± 47.87 (cycles) for the 4-strand double modified Kessler technique and 34.56 ± 6.60 (N) and 189 ± 66.36 (cycles) for our new continuous double knots technique. The T-test revealed a significant difference between the 2 techniques (p < 0.05). In terms of biomechanical properties in tendon repair, the continuous double knots technique group had a higher tensile strength than the 4-strand double-modified Kessler technique group. There were also significant differences between the ultimate failure load and cyclic testing to failure for the flexor tendon sutures.

Conclusions
The continuous double knots technique suture technique had significantly higher maximum tensile strength and cyclic testing than the 4-strand double modified Kessler technique in an in vitro study, and in thus an optional technique for flexor tendon repair.
A novel decrystallizing protein CxEXL22 from Arthrobotrys sp. CX1 capable of synergistically hydrolyzing cellulose with cellulases
Authors: Li Rong , Sun Yunze , Zhou Yihao , Gai Jiawei , You Linlu , Yang Fan , Tang Wenzhu , Li Xianzhen 
Published: September 2021

AbstractA novel expansin-like protein (CxEXL22) has been identified and characterized from newly isolated Arthrobotrys sp. CX1 that can cause cellulose decrystallization. Unlike previously reported expansin-like proteins from microbes, CxEXL22 has a parallel β-sheet domain at the N terminal, containing many hydrophobic residues to form the hydrophobic surface as part of the groove. The direct phylogenetic relationship implied the genetic transfers occurred from nematode to nematicidal fungal Arthrobotrys sp. CX1. CxEXL22 showed strong activity for the hydrolysis of hydrogen bonds between cellulose molecules, especially when highly crystalline cellulose was used as substrate. The hydrolysis efficiency of Avicel was increased 7.9-fold after pretreating with CxEXL22. The rupture characterization of crystalline region indicated that CxEXL22 strongly binds cellulose and breaks up hydrogen bonds in the crystalline regions of cellulose to split cellulose chains, causing significant depolymerization to expose much more microfibrils and enhances cellulose accessibility.
Differentiating prostate cancer from benign prostatic hyperplasia using whole-lesion histogram and texture analysis of diffusion- and T2-weighted imaging
Authors: Xing Pengyi , Chen Luguang , Yang Qingsong , Song Tao , Ma Chao , Grimm Robert , Fu Caixia , Wang Tiegong , Peng Wenjia , Lu Jianping 
Published: September 2021

AbstractBackgroundTo explore the usefulness of analyzing histograms and textures of apparent diffusion coefficient (ADC) maps and T2-weighted (T2W) images to differentiate prostatic cancer (PCa) from benign prostatic hyperplasia (BPH) using histopathology as the reference.MethodsNinety patients with PCa and 112 patients with BPH were included in this retrospective study. Differences in whole-lesion histograms and texture parameters of ADC maps and T2W images between PCa and BPH patients were evaluated using the independent samples t-test. The diagnostic performance of ADC maps and T2W images in being able to differentiate PCa from BPH was assessed using receiver operating characteristic (ROC) curves.Results The mean, median, 5th, and 95thpercentiles of ADC values in images from PCa patients were significantly lower than those from BPH patients (p < 0.05). Significant differences were observed in the means, standard deviations, medians, kurtosis, skewness, and 5thpercentile values of T2W image between PCa and BPH patients (p < 0.05). The ADC5thshowed the largest AUC (0.906) with a sensitivity of 83.3 % and specificity of 89.3 %. The diagnostic performance of the T2W image histogram and texture analysis was moderate and had the largest AUC of 0.634 for T2WKurtosiswith a sensitivity and specificity of 48.9% and 79.5 %, respectively. The diagnostic performance of the combined ADC5th& T2WKurtosisparameters was also similar to that of the ADC5th& ADCDiff−Variance.ConclusionsHistogram and texture parameters derived from the ADC maps and T2W images for entire prostatic lesions could be used as imaging biomarkers to differentiate PCa and BPH biologic characteristics, however, histogram parameters outperformed texture parameters in the diagnostic performance.
The “Fracking” technique: a novel approach to crack deep calcified plaque in the common femoral artery with hydraulic pressure
Authors: Haraguchi Takuya , Fujita Tsutomu , Kashima Yoshifumi , Tsujimoto Masanaga , Takeuchi Tsuyoshi , Tadano Yutaka , Hachinohe Daisuke , Kaneko Umihiko , Kobayashi Ken , Kanno Daitaro , Sato Katsuhiko 
Published: September 2021

Abstract
Background
The patency achieved by conventional peripheral interventions for atherosclerotic lesions in the common femoral artery (CFA), called the “no stenting zone”, is not superior to that achieved by surgical endarterectomy due to calcified plaque occupying the area. Plaque modification strategies to obtain acute gain in CFA patency provide the better clinical outcomes than standard balloon angioplasty. Atherectomy devices, which focus on the modification of superficial calcifications, contribute to the improvement of clinical outcomes. However, deep calcifications resist vessel expansion such that luminal gain is not easily achieved.

Main text
We propose a novel calcified plaque modification technique, named the “fracking technique” (FT). The term fracking refers to how a rock is fractured by the high hydraulic pressure. In this technique, deep calcifications are cracked with hydraulic pressure via a balloon indeflator through an 18-gauge needle, which punctures calcifications to achieve greater acute luminal gain. Case 1 involved an 81-year-old male with eccentric calcified plaque in the right CFA. Conventional balloon angioplasty for the lesion yielded a suboptimal minimal lumen area (MLA), which increased from 6.2 to 10.7-mm2 on intravascular ultrasound (IVUS). The FT was implemented to obtain a larger MLA. After the FT was repeated at three locations at up to 8-atm, a greater MLA of 27.1-mm2 was achieved without complications. Case 2 involved a 72-year-old male undergoing hemodialysis due to diabetes mellitus who presented with ischemic pain in his right limbs at rest due to severe stenosis with eccentric calcification in the distal CFA. The MLA on IVUS before and after balloon angioplasty was 10.0-mm2 and 13.1-mm2, respectively, and this result was still suboptimal. The FT was attempted and successfully yielded a greater MLA of 28.9-mm2 without complications. Restenosis has not been detected for 2 years follow-up period.

Conclusions
The FT is an effective option for treating calcified CFA lesions to achieve a larger lumen area. Long-term follow-up studies are necessary.
Agent based coordination protocol for system of cyber-physical systems
Authors: Umair Aisha , Clausen Anders , Demazeau Yves , Jørgensen Bo Nørregaard 
Published: September 2021

AbstractNowadays, society and business rely heavily on Information and Communication Technology (ICT) systems, which are progressing faster than ever. To stay on pace with them, focus is shifted towards integration of individual ICT systems into complex systems, which offers more functionality than simply the sum of individual systems. In this regard, Cyber-Physical Systems (CPSs) have gained significant importance and System-of-Systems (SoS) approach has been suggested for modeling complex CPSs to achieve a higher level goal, by dynamically building a large system with existing autonomous, and heterogeneous constituent systems (CSs). An important challenge in a system of Cyber-Physical Systems (SoCPSs) is to develop seamless collaboration between autonomous constituent-CPSs (CCPSs) to coordinate their operations. In this paper, we propose an agent based coordination mechanism to coordinate resource allocation and demand in SoCPSs. The approach models each CCPS as an agent and describes how multiple autonomous CCPSs, i.e., Virtual Power Plant (VPP), Commercial Greenhouse Growers (CGGs), communicate and collaborate with each other asynchronously through negotiation and how potential conflicts between CCPSs with conflicting goals are resolved. The efficacy of the proposed mechanism is validated through simulation of different real-world acyclic SoCPSs topologies. The results show that proposed approach is able to balance the individual requirements of multiple connected CPSs while achieving SoCPSs’ mission.
The Fertility of Immigrants From Low-Fertility Settings: Adaptation in the Quantum and Tempo of Childbearing?
Authors: Mussino Eleonora , Wilson Ben , Andersson Gunnar 
Published: September 2021

Abstract
Immigrant women who have lived longer in a destination often have relatively low levels of fertility, which is sometimes taken as evidence of the adaptation of behavior. This evidence is almost exclusively based on studies of immigrants from high-fertility settings, while the fertility of immigrants from low-fertility settings has been largely overlooked. Research has also rarely studied the fertility of immigrants who migrated as children, despite the methodological advantages of applying such an approach. This study focuses on women who grew up in Sweden with a migration background from low-fertility origins. We expect that Sweden's welfare regime makes it easier for women to combine childbearing and working life, regardless of migration background, thereby facilitating an adaptation of fertility behavior toward that prevailing in Sweden. We find evidence of adaptation in terms of birth timing for at least half of the country-origin groups that we study, but very little evidence of adaptation in terms of completed fertility. Further, we find that, in comparison with ancestral Swedes, completed fertility differentials are larger for second-generation individuals than for immigrants who arrived during childhood. This is evidence against the notion of “straight-line” adaptation for immigrants and the children of immigrants who are born in Sweden.
The Effect of EITC Exposure in Childhood on Marriage and Early Childbearing
Authors: Michelmore Katherine , Lopoo Leonard M. 
Published: September 2021

Abstract
Using data from the Panel Study of Income Dynamics, this study analyzes the effect of exposure to the Earned Income Tax Credit (EITC) in childhood on marriage and childbearing in early adulthood. Results suggest that EITC exposure in childhood leads women to delay marriage and first births in early adulthood (ages 16–25), but has no effect on men. A $1,000 increase in EITC exposure in childhood leads to a 2%–3% decline in a woman's likelihood of having a first birth and a comparable decline in her likelihood of marrying by her early 20s. We find similar reductions in fertility among Black and White women, though marriage declines are concentrated among White women. Results are focused on children growing up in the bottom half of the income distribution and those who spent the majority of childhood residing with a single parent—two groups that are the primary beneficiaries of the EITC. These findings have important implications for the well-being of individuals exposed to the EITC in childhood, as well as their future children.
Phosphorylation-dependent routing of RLP44 towards brassinosteroid or phytosulfokine signalling
Authors: Garnelo Gómez Borja , Holzwart Eleonore , Shi Chaonan , Lozano-Durán Rosa , Wolf Sebastian 
Published: September 2021

ABSTRACT
Plants rely on cell surface receptors to integrate developmental and environmental cues into behaviour adapted to the conditions. The largest group of these receptors, leucine-rich repeat receptor-like kinases, form a complex interaction network that is modulated and extended by receptor-like proteins. This raises the question of how specific outputs can be generated when receptor proteins are engaged in a plethora of promiscuous interactions. RECEPTOR-LIKE PROTEIN 44 (RLP44) acts to promote both brassinosteroid and phytosulfokine signalling, which orchestrate diverse cellular responses. However, it is unclear how these activities are coordinated. Here, we show that RLP44 is phosphorylated in its highly conserved cytosolic tail and that this post-translational modification governs its subcellular localization. Whereas phosphorylation is essential for brassinosteroid-associated functions of RLP44, its role in phytosulfokine signalling is not affected by phospho-status. Detailed mutational analysis suggests that phospho-charge, rather than modification of individual amino acids determines routing of RLP44 to its target receptor complexes, providing a framework to understand how a common component of different receptor complexes can get specifically engaged in a particular signalling pathway.
A palmitoylation code controls PI4KIIIα complex formation and PI(4,5)P2 homeostasis at the plasma membrane
Authors: Batrouni Alex G. , Bag Nirmalya , Phan Henry T. , Baird Barbara A. , Baskin Jeremy M. 
Published: September 2021

ABSTRACT
Phosphatidylinositol 4-kinase IIIα (PI4KIIIα) is the major enzyme responsible for generating phosphatidylinositol (4)-phosphate [PI(4)P] at the plasma membrane. This lipid kinase forms two multicomponent complexes, both including a palmitoylated anchor, EFR3. Whereas both PI4KIIIα complexes support production of PI(4)P, the distinct functions of each complex and mechanisms underlying the interplay between them remain unknown. Here, we present roles for differential palmitoylation patterns within a tri-cysteine motif in EFR3B (Cys5, Cys7 and Cys8) in controlling the distribution of PI4KIIIα between these two complexes at the plasma membrane and corresponding functions in phosphoinositide homeostasis. Spacing of palmitoyl groups within three doubly palmitoylated EFR3B ‘lipoforms’ affects both interactions between EFR3B and TMEM150A, a transmembrane protein governing formation of a PI4KIIIα complex functioning in rapid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] resynthesis following phospholipase C signaling, and EFR3B partitioning within liquid-ordered and -disordered regions of the plasma membrane. This work identifies a palmitoylation code involved in controlling protein–protein and protein–lipid interactions that affect a plasma membrane-resident lipid biosynthetic pathway.
Electrochemical Sensing Applications Using Diamond Microelectrodes
Authors: Ivandini Tribidasari A , Einaga Yasuaki 
Published: September 2021

Abstract
The miniaturization of boron-doped diamond (BDD) electrodes is an important requirement for application to the study of electrochemical processes in living beings. In this work, we describe the fabrication and characterization of BDD electrodes with micrometer dimensions, with a particular emphasis on micro needle electrodes. As a result of the combination of the microelectrode size effect and the intrinsic properties of the diamond films, these electrodes showed not only a significantly lower background current than diamond macroelectrodes, but also an ability for use in analytical sensing in low conductive media. Accordingly, a wider range of experiments including in vivo measurements could be performed.
The effect of non-pharmacologic strategies on prevention or management of intensive care unit delirium: a systematic review
Authors: Cupka Julie S , Hashemighouchani Haleh , Lipori Jessica , Ruppert Matthew M. , Bhaskar Ria , Ozrazgat-Baslanti Tezcan , Rashidi Parisa , Bihorac Azra 
Published: September 2021

Background: Post-operative delirium is a common complication among adult patients in the intensive care unit. Current literature does not support the use of pharmacologic measures to manage this condition, and several studies explore the potential for the use of non-pharmacologic methods such as early mobility plans or environmental modifications. The aim of this systematic review is to examine and report on recently available literature evaluating the relationship between non-pharmacologic management strategies and the reduction of delirium in the intensive care unit. Methods: Six major research databases were systematically searched for articles analyzing the efficacy of non-pharmacologic delirium interventions in the past five years. Search results were restricted to adult human patients aged 18 years or older in the intensive care unit setting, excluding terminally ill subjects and withdrawal-related delirium. Following title, abstract, and full text review, 27 articles fulfilled the inclusion criteria and are included in this report. Results: The 27 reviewed articles consist of 12 interventions with a single-component investigational approach, and 15 with multi-component bundled protocols. Delirium incidence was the most commonly assessed outcome followed by duration. Family visitation was the most effective individual intervention while mobility interventions were the least effective. Two of the three family studies significantly reduced delirium incidence, while one in five mobility studies did the same. Multi-component bundle approaches were the most effective of all; of the reviewed studies, eight of 11 bundles significantly improved delirium incidence and seven of eight bundles decreased the duration of delirium. Conclusions: Multi-component, bundled interventions were more effective at managing intensive care unit delirium than those utilizing an approach with a single interventional element. Although better management of this condition suggests a decrease in resource burden and improvement in patient outcomes, comparative research should be performed to identify the importance of specific bundle elements.
Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus
Authors: Amrullah Amrullah , Wahidah Wahidah , Ardiansyah Ardiansyah , Indrayani Indrayani 
Published: September 2021

Background: Vaccination is an effective and alternative means of disease prevention, however, it cannot be conducted on the offspring of fish. For this process to take place, the transfer of maternal immunity must be implemented. This study aims to determine the effectiveness of transferring immunity from the broodstock to the offspring using a polyvalent vaccine against Aeromonas hydrophila, Streptococcus agalactiae, and Pseudomonas fluorescens in Nile tilapia, Oreochromis niloticus. Methods: Nile tilapia broodstock, with an average weight of 203g (±SD 23 g) was injected with a vaccine used as a treatment. Example include A. hydrophila monovalent (MA), S. agalactiae monovalent (MS), P. fluorescens monovalent (MP), A. hydrophila and S. agalactiae bivalent (BAS), A. hydrophila and P. fluorescens bivalent (BAP), P. fluorescens and S. agalactiae bivalent (BPS), and A. hydrophila, S. agalactiae, and P. fluorescens polyvalent vaccines (PAPS). While the control was fish that were injected with a PBS solution. The broodstock’s immune response was observed on the 7th, 14th, 21st, and 28th day, while the immune response and challenge test on the offspring was conducted on the 10th, 20th, 30th, and 40th day during the post-hatching period. Result: The application of PAPS in broodstock could significantly induce the best immune response and immunity to multiple diseases compared to other treatments. The RPS of the PAPS was also higher than the other types of vaccines. This showed that the transfer of immunity from the broodstock to the Nile tilapia offspring could protect it against bacterial diseases such as A. hydrophila, S. agalactiae, and P. fluorescens. Conclusion: The application of PAPS A. hydrophila, S. agalactiae, P. fluorescens vaccines increased the broodstock’s immune response and it was transferred to their offsprings. They were able to produce tilapia seeds that are immune to diseases caused by A. hydrophila, S. agalactiae, and P. fluorescens.