bluuebunny/crossref_metadata_2025_split huggingface dataset:
Projected shifts in loggerhead sea turtle habitat in the Northwest Atlantic Ocean due to climate change
Authors: Patel Samir , Winton Megan , Hatch Joshua , Haas Heather , Saba Vincent , Fay Gavin , Smolowitz Ronald 
Published: January 2021

Abstract
It is well established that sea turtles are vulnerable to atmospheric and oceanographic shifts associated with climate change. However, few studies have formally projected how their seasonal marine habitat may shift in response to warming ocean temperatures. Here we used a high-resolution global climate model and a large satellite tagging dataset to project changes in the future distribution of suitable thermal habitat for loggerheads along the northeastern continental shelf of the United States. Between 2009 and 2018, we deployed 196 satellite tags on loggerheads within the Middle Atlantic Bight (MAB) of the Northwest Atlantic continental shelf region, a seasonal foraging area. Tag location data combined with depth and remotely sensed sea surface temperature (SST) were used to characterize the species’ current thermal range in the MAB. The best-fitting model indicated that the habitat envelope for tagged loggerheads consisted of SST ranging from 11.0° - 29.7° C and depths between 0 – 105.0 m. The calculated core habitat consisted of temperatures between 15.0° – 28.0° C and at depths between 8.0 – 92.0 m and the highest probability of presence occurred in regions with SST between 17.7° – 25.3° C and at depths between 26.1 – 74.2 m. The habitat suitability model was then forced by a high-resolution global climate model under a doubling of atmospheric CO2 to project loggerhead probability of presence over the next 80 years. Our results suggest that loggerhead thermal habitat and seasonal duration will likely increase in northern regions of the NW Atlantic shelf. This change in spatiotemporal range for sea turtles in a region of high anthropogenic use may prompt adjustments to the localized protected species conservation measures.
Qualitative Interviews on The Diagnosis Process of Hepatitis B Among 50 Patients in Chongqing, China
Authors: zhou xiangxi , zhang fan , Ao yong , lu chun , li ting , xu xiang , zeng huan 
Published: January 2021

Abstract
Objective: To identify the obstacles preventing and controlling hepatitis B and put forward suggestions for its prevention and treatment by describing the discovery process of hepatitis B patients. Methods: We conducted face-to-face interviews with 50 hepatitis B patients in Chongqing, China from July to August 2019 by constructing a thematic analysis framework, which included interviewees’ social demographic characteristics, diagnosis approach, signs and symptoms at the time of discovery, feelings after diagnosis, and doctor’s instructions.Results: Most of the patients were firstly found hepatitis B via physical examination when patients were asymptomatic or had no obvious symptoms. The majority of them were shocked, frightened, and at a loss when they were diagnosed. The patients could remember doctor’s instructions on keeping healthy lifestyle, but they had very little impression about doctors’ reminding about regular liver function test. Failure of regular follow-up to liver function test brought irreversible loss for certain patients.Conclusions: Most of the patients were found hepatitis B passively. The patients need professional mental help after the diagnosis to overcome the negative emotion. The doctors’ instruction should emphasize more on the importance of regular follow-up to liver function test besides health lifestyle.
A Bibliometric Analysis of Researches on Flap Endonuclease 1 From 2005 to 2019
Authors: Wei Qiaochu , Shen Jiming , Wang Dongni , Han Xu , Shi Jing , Zhao Lei , Teng Yuee 
Published: January 2021

Abstract
Background: Flap endonuclease 1 (FEN1) is a structure-specific nuclease that plays a role in a variety of DNA metabolism processes. FEN1 is important for maintaining genomic stability and regulating cell growth and development. It is associated with the occurrence and development of several diseases, especially cancers. There is a lack of systematic bibliometric analyses focusing on research trends and knowledge structures related to FEN1.Purpose: To analyze hotspots, the current state and research frontiers performed for FEN1 over the past 15 years. Methods: Publications were retrieved from the Web of Science Core Collection (WoSCC) database, analyzing publication dates ranging from 2005 to 2019. VOSviewer1.6.15 and Citespace5.7 R1 were used to perform a bibliometric analysis in terms of countries, institutions, authors, journals and research areas related to FEN1. A total of 421 publications were included in this analysis. Results: Our findings indicated that FEN1 has received more attention and interest from researchers in the past 15 years. Institutes in the United States, specifically the Beckman Research Institute of City of Hope published the most research related to FEN1. SHEN BH，ZHENG L and BAMBARA RA were the most active researchers investigating this endonuclease and most of this research was published in the Journal of Biological Chemistry. The main scientific areas of FEN1 were related to biochemistry, molecular biology，cell biology，genetics and oncology. Research hotspots included biological activities, DNA metabolism mechanisms, protein-protein interactions and gene mutations. Research frontiers included oxidative stress, phosphorylation and tumor progression and treatment. Conclusion: This bibliometric study may aid researchers in the understanding of the knowledge base and research frontiers associated with FEN1. In addition, emerging hotspots for research can be used as the subjects of future studies.
Treatment of Atrophic Rhinitis with Manuka Honey–a non-randomized control trial, and a new theory of etiopathogenesis for the disease.
Authors: Sarkar Saurav , Purkait Suvendu , Maiti Rituparna , Sharma Prity , Venugopal Giriprasad , Mutha Naresh , Ramadass Balamurugan 
Published: January 2021

Abstract
Exact etiology of Atrophic rhinitis (AtR) is yet unknown. Polygenic and polybacterial causes have been implicated in the onset and progression of this disease. AtR doesn’t respond to any particular modality of treatment that targets specific etiology, this describes its multifactorial nature. In this study, we report on a non-randomized control trial on the use of a nasal spray of 10% Manuka honey in patients with AtR attending the out-patient unit of the Department of Otorhinolaryngology and Head Neck Surgery. In this study, we show significant observations: 1. Decreased fetid smell, 2. Thickening of the mucosa, 3. Decreased inflammation with healed mucosal ulcers, 4. Increased concentration of the mucosal glands, 5. Alteration in Nasal microbiome, and 6. Increased expression of SCFA receptors. These changes occurred in response to honey therapy, and are consequent to the resetting of the Nasal microbiome.
Predicting High Energy Density Materials using Abinitio Evolutionary Algorithms: The Results for N5AsF6
Authors: Benchafia El Mostafa , Wang Xianqin , Iqbal Zafar , Abedrabbo Sufian 
Published: January 2021

Abstract
N5AsF6 is the first successfully synthesized salt that has a polymeric nitrogen moeity (N5+). Although 12 other N5+ salts followed, with N5SbF6 and N5Sb2F11 being the most stable, the crystal structure of N5AsF6 remains unknown. Currently, it is impossible to experimentally determine the structures of N5AsF6 due to its marginal stability and explosive nature. Here, following an ab initio evolutionary prediction and using only the stoichiometry of N5AsF6 as a starting point, we were able to reveal the crystal structure of this high energy density material (HEDM). The C2V symmetry of the N5+ cation, as suggested from earlier investigations, is confirmed to be the symmetry adopted by this polymeric nitrogen within the crystal. This result gave full confidence in the validity of this crystal prediction approach. While stability of the N5+ within the crystal is found to be driven by electronic considerations, the marginal stability of this HEDM is found to be related to a partial softening of its phonon modes.
Cholinesterase activity in serum during general anesthesia in health and disease
Authors: Sinai Yitzhak Brzezinski , Zwang Ester , Plotnikova Elena , Halizov Esti , Shapira Itzhak , Zeltser David , Rogowski Ori , Berliner Shlomo , Matot Idit , Shenhar-Tsarfaty Shani 
Published: January 2021

Abstract
Atherosclerotic patients challenge the anesthesiologist as they display a significant instability between the two parts of the autonomic nervous system. We aimed to assess the effect of general anesthesia (GA) and surgery on serum cholinesterase activity. Prospective study of 57 patients undergoing ambulatory or vascular surgery under GA. Cholinesterase levels were measured before induction of anesthesia, 15 minutes thereafter and at the end of surgery by measuring the capacity of serum Acetylcholinesterase (AChE) and Butyrylcholinesterase to hydrolyze AcetylThioCholine. Data of atherosclerotic disease, anesthesia management were analyzed.Both AChE and total Cholinergic Status (CS) decreased significantly after GA induction at 15 minutes and furthermore by the end of surgery. Vascular surgery patients, presented lower baseline cholinesterase activity compared to patients for ambulatory surgery. In patients requiring intraoperative administration of phenylephrine for hemodynamic support (21.1%), a significant lower level of AChE and CS was observed compared to untreated patients. A positive correlation was found between the lowest temperatures measured and the AChE and CS change from the baseline values. Our findings serve as a mirror to the sympathetic/parasympathetic disbalance during GA with a marked decrease in the parasympathetic tone. Our data show that low cholinesterase activity increase the need for hemodynamic support.
A Novel External Fixator to Facilitate Accurate and Efficient Fracture Treatment
Authors: Shen Mingkui , Ai Zizheng , Li Jingna , Liang Huaping , Dong Xieping 
Published: January 2021

Abstract
Background: The rapid popularization and application of bone external fixation technology has led to external fixators emerging as remarkable methods to treat various complex fractures. Because of their instability, assembly complexity and poor controllability, however, new external fixators are challenging to design in terms of facilitating accurate and efficient fracture treatment.Methods: We designed a novel external fixator that could feasibly treat clinical complex fractures. A prospective study was conducted on patients with various complex fractures from September 2017 to September 2019. During this period, we treated 24 patients with a universal joint external fixator and then evaluated its therapeutic effects. In this study, adult cadaver femur fracture fixations were modelled by using universal joint (UJ) and AO external fixators, and the mechanical stability was assessed by applying a biomechanical testing device.Results: The designed device not only fits the repair site but could also be conveniently installed and implanted. All patients achieved good fixation and fracture healing with the universal joint fracture external fixators. In in vitro biomechanical testing, constructs were loaded under axial compression, lateral compression and torsion. Overall axial stiffness showed no significant differences between the two groups. The lateral stiffness was 1.554 ± 0.017 mm for the UJ group and 1.342 ± 0.020 mm for the AO group (p<0.001) under a 600 N load, while the torsional stiffness was 15.727 ± 0.141° for the UJ group and 14.472 ± 0.292° for the AO group under a 40 Nm load.Conclusion: In this small and preliminary study, the universal joint external fixator, which achieved a stable mechanical structure, was capable of intensive and efficient treatment for comprehensive fractures. If these findings can be confirmed in a larger study, this external fixator may be widely used in certain situations where traditional external fixators are not a convenient option.
Predictors of Treatment Failure Among Patients With Gunshot Wounds and Post-Traumatic Stress Disorder
Authors: Kuchyn Iurii , Horoshko Vasyl' 
Published: January 2021

Abstract
Background: The 82.1% treatment failure of post-traumatic stress disorder(PTSD), associated with gunshot wounds, is related to high incidence of chronic pain syndrome as well as resistance to the PTSD treatment. Defining treatment failure predictors among the PTSD patients with gunshot extremity wounds and the following therapy would improve treatment outcomes. Methods: A total of 218 patients completed the study. The Mississippi Scale for Combat-Related PTSD (M-PTSD) was used for assessment of the treatment outcome rate. The risk relation between treatment failure and factors was assessed by a univariate or multivariate logistic regression method, with the model accuracy measured by the AUC – Area under the ROC curve. The odds ratio (OR) was considered for the qualitative factor assessment. Results: The predictors of the PTSD treatment failure among the patients with gunshot wounds to the extremities are: 1) anesthesia type: the risk of failure is higher with the general anesthesia compared to the regional (p=0.002), OR=0.30 (95% CI 0.13-0.69) and the regional one with sedation (p=0.004), OR=0.30 (95% CI 0,14-0.65); 2) severe postoperative pain: the risk of treatment failure rises with increased pain intensity assessed by the visual analogue scale (p=0.02), OR=3.2 (95% CI 1.2-8.3). Conclusions: The analysis showed that administration of general anesthesia compared to the regional one (regardless of the sedation) and high postoperative pain intensity are associated with higher risk of the PTSD treatment failure among patients with gunshot wounds to the extremities. The preference of regional anesthesia and postoperative pain control may potentially improve the treatment outcomes. Trial registration ClinicalTrials.gov: Retrospectively registered on December 28, 2020, NCT04689022
Improvement and Prediction of Secondary Metabolites Biosynthesis Under Yeast Extract Elicitation of Neem Cell Suspension Culture Using Response Surface Methodology
Authors: Farjaminezhad Reza , Garoosi Ghasemali 
Published: January 2021

Abstract
Neem is the medicinal plant used as antimalarial, antibacterial, antiviral, insecticide and antimicrobial. The aim of this study was investigate effect of yeast extract and sampling time on cell growth, secondary metabolites synthesis, SQS1 and MOF1 genes expression and prediction of secondary metabolites synthesis by response surface methodology. Highest fresh and dry cell weight were 580.25 g/L and 21.01 g/L obtained 6 days after addition 100 mg/L yeast extract. Highest azadirachtin accumulation and production were 16.08 mg/g DW and 219.78 mg/L obtained at 25 mg/L yeast extract for 2 and 4 days. Maximum mevalonic acid accumulation and production were 1.746 mg/g DW and 23.77 mg/L observed 2 days after addition 50 mg/L yeast extract addition. Highest amount of squalene accumulation (0.215 mg/g DW) and production (4.526 mg/L) were achieved 4 days after application of 50 mg/L yeast extract. Prediction results showed that highest azadirachtin accumulation (13.607 mg/g DW) and production (190.50 mg/L), mevalonic acid accumulation (0.500 mg/g DW) and production (5.57 mg/L) and squalene accumulation (0.301 mg/g DW) obtained by using 245 mg/L yeast extract for 2 days, 71 mg/L yeast extract for 2 days, 200 mg/L yeast extract for 4.96 days, whitout yeast extract for 6.54 days and 4 days, respectively and predicted that highest squalene production is achieved by long-term exposure to high concentrations of yeast extract. qRT-PCR analysis showed the maximum relative gene expression of SQS1 and MOF1 by addition 150 mg/L yeast extract for 4 days and 25 mg/L yeast extract for 2 days, respectively.
Spin Structure Factors of Doped Monolayer Germanene in the Presence of Spin-Orbit Coupling
Authors: rezania hamed , azizi farshad 
Published: January 2021

Abstract
In this paper, we present a Kane-Mele model in the presence of magnetic field and next nearest neighbors hopping amplitudes for investigations of the spin susceptibilities of Germanene layer.Green's function approach has been implemented to find the behavior of dynamical spin susceptibilities of Germanene layer within linear response theory and in the presence of magnetic field and spin-orbit coupling at finite temperature.Our results show the magnetic excitation mode for both longitudinal and transverse components of spin tends to higher frequencies with spin-orbit coupling strength.Moreover the frequency positions of sharp peaks in longitudinal dynamical spin susceptibility are not affected by variation of magnetic field while the peaks in transverse dynamical susceptibility moves to lower frequencies with magnetic field.The effects of electron doping on frequency behaviors of spin susceptibilities have been addressed in details.Finally the temperature dependence of static spin structure factors due to the effects of spin-orbit coupling, magnetic field and chemical potential has been studied.
Safety and Effectiveness of High-Dose Vitamin C in Patients with COVID-19; A Randomized Controlled open-label Clinical Trial ‎
Authors: JamaliMoghadamSiahkali Saeidreza , Zarezade Besharat , Koolaji Sogol , Alinaghi Seyed Ahmad Seyed , Zendehdel Abolfazl , Tabarestani Mohammad , Moghadam Ehsan Sekhavati , Abbasian Ladan , Manshavi Sayed Ali Dehghan , Salehi Mohamadreza , Hasannezhad Malihe , Ghaderkhani Sara , Meidani Mohsen , Salahshour Faeze , Jafari Fatemeh , Manafi Navid , Ghiasvand Fereshteh 
Published: January 2021

Abstract
Background: To assess the effectiveness of vitamin C treatment against coronavirus disease 2019 (COVID-19)Methods: An open-label, randomized, and controlled trial was conducted on patients with severe COVID-19 infection. The case and control treatment groups each consisted of 30 patients. The control group received lopinavir/ritonavir and hydroxychloroquine and the case group received high-dose of vitamin C (six gr daily) added to the same regimen. Results: There were no statistically significant differences between two groups with respect to age and gender, laboratory results, and underlying diseases. On the 3rd day of hospitalization, the mean core body temperatures was significantly lower and SpO2 was higher In the case group (p value = 0.001, and 0.014, respectively). The median length of hospitalization in case group which was significantly longer than the control group (8.5 days vs. 6.5 days) (p value = 0.0280). There was no significant difference in SpO2 levels at discharge time, the length of ICU stay, and mortality between the two groups.Conclusions: We did not find significantly better outcomes in the group who were treated with high-dose vitamin C in addition to the main treatment regimen at discharge.Trial registration: The project was registered by Iranian Registry of Clinical Trials.IRCT20200411047025N1
Diabetes Outbreak During COVID19 Lock-down in a Prediabetic Patient with Cystic Fibrosis Long Treated With Glargine
Authors: Rosanio Francesco Maria , Mozzillo Enza , Cimbalo Chiara , Casertano Alberto , Sepe Angela , Raia Valeria , Franzese Adriana , Tosco Antonella 
Published: January 2021

Abstract
Background: Cystic Fibrosis Related Diabetes (CFRD) is a frequent comorbidity of patients with Cystic Fibrosis (CF). A worsening of clinical conditions appears before CFRD. It has been demonstrated a decline in pulmonary function and nutritional status also in patients with prediabetes. Few trials show that insulin may be beneficial in prediabetic CF patients, to date guidelines do not recommended for this condition. Case presentation: we report a case of a patient treated with insulin glargine at 13, due to glycemic intolerance, and with Lumacaftor/Ivacaftor at 15. A reduction of pulmonary exacerbations was observed after glargine therapy, also confirmed after the starting of Lumacaftor/ Ivacaftor in this patient. Pulmonary function improved only after the first year of glargine therapy, then a deterioration appeared due to the natural history of CF lung damage. During the COVID-19 lockdown, poor adherence to care contributed to diabetes onset needing high insulin requirements. After two weeks the patient returned to prediabetic condition and his previous dose of glargine. Conclusions: our case highlights firstly that insulin glargine has contributed to preserve him from further clinical worsening due to prediabetes in the years before pandemic, secondly the negative impact of COVID-19 lockdown on the clinical course of a chronic disease as CF.
Comprehensive Analysis of Autophagy-related Genes in Hepatocellular Carcinoma
Authors: Wang Li , Qu Jialin , Zhou Na , Jiang Man , Zhang Xiaochun 
Published: January 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is the common type of cause of cancer-related death among human cancers. There are ample evidences to showing that autophagy-related genes (ARGs) may play a significant role in the biological process of HCC. Methods: In this study, we aim to identify survival model and nomogram that could effectively predict the prognosis of HCC based on ARGs. First, we download the data of HCC patients from TCGA database. Second, we analysis the function of ARGs by utilized GO and the KEGG analysis. Finally, we screen 5 ARGs (SQSTM1, CAPN10, EIF2S1, ATIC, RHEB) for survival model by performed the Cox regression and Lasso regression analysis. We further built and verified a prognostic nomogram base on prognostic ARGs. Moreover, its efficacy was validated by the ICGC database. The expressions level of 5 ARGs was performed using Oncomine database, the Human Protein Atlas and Kaplan-Meier plotter.Result: We found patients the survival of patients in the different groups was significantly different both in the TCGA cohort and ICGC cohort. The survival model showed good performance for predicting the prognosis of HCC patients by having a better area under the receiver operating characteristic curve than other clinicopathological parameters. Conclusion: our survival models and prognostic ARGs nomogram can be independent risk factors for hepatocellular carcinoma patients.