bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling the spatial distribution of mycetoma in Sudan
Authors: Hassan Rowa , Simpson Hope , Cano Jorge , Bakhiet Sahar , Ganawa Eltayeb , Argaw Daniel , Newport Melanie J , Deribe Kebede , Fahal Ahmed Hassan 
Published: April 2021

Abstract

Background
Mycetoma is a neglected tropical disease that is reported worldwide and Sudan has the highest reported number of mycetoma infections across the globe. The incidence, prevalence and burden of mycetoma globally are not precisely known and its risk factors remain largely unelucidated.

Methods
This study aimed to identify the environmental predictors of fungal and bacterial mycetoma in Sudan and to identify areas of the country where these niche predictors are met. Demographic and clinical data from confirmed mycetoma patients seen at the Mycetoma Research Centre from 1991 to 2018 were included in this study. Regression and machine learning techniques were used to model the relationships between mycetoma occurrence in Sudan and environmental predictors.

Results
The strongest predictors of mycetoma occurrence were aridity, proximity to water, low soil calcium and sodium concentrations and the distribution of various species of thorny trees. The models predicted the occurrence of eumycetoma and actinomycetoma in the central and southeastern states of Sudan and along the Nile river valley and its tributaries.

Conclusion
Our results showed that the risk of mycetoma in Sudan varies geographically and is linked to identifiable environmental risk factors. Suitability maps are intended to guide health authorities, academic institutes and organisations involved in planning national scale surveys for early case detection and management, leading to better patient treatment, prevention and control of mycetoma.
Genetic, bioacoustic and morphological analyses reveal cryptic speciation in the warbling vireo complex (Vireo gilvus: Vireonidae: Passeriformes)
Authors: Carpenter Amanda M , Graham Brendan A , Spellman Garth M , Klicka John , Burg Theresa M 
Published: April 2021

AbstractCryptic species are closely related taxa that are difficult to separate morphologically, but are reproductively isolated. Here we examine the warbling vireo complex (Vireo gilvus), a widespread songbird speculated to be comprised of more than one cryptic species. We included three taxa within the complex: two of the western (Vireo gilvus swainsonii and Vireo gilvus brewsteri) subspecies and the single eastern (Vireo gilvus gilvus) subspecies. We used mtDNA and microsatellite loci to assess the congruence of genetic data to the current subspecies boundaries. We then incorporated bioacoustic, morphometric and ecological niche modelling analyses to further examine differences. We found two genetic groups with mtDNA analysis, splitting eastern and western warbling vireos. Microsatellite analyses revealed four genetic groups: an eastern group, a Black Hills group and two western groups that do not agree with current western subspecies boundaries based on phenotypic data. Our results suggest that eastern and western warbling vireos have been reproductively isolated for a long period of time and therefore may be best treated as separate species. However, more research into areas of contact to examine the presence of hybridization is advised before making a taxonomic revision. Differences between the two western genetic groups appear less clear, requiring additional research.
Escaping the Classroom: Replacing Traditional Lecture With Interactive Learning Stations in Nursing Education
Authors: Stringfellow Elizabeth Joy 
Published: April 2021

Abstract
The daily evolution of the nursing profession has charged nurse educators to be innovative and creative in their teaching strategies, with the goal of engaging and motivating students and promoting lifelong learning. Educational escape rooms use multiple teaching/learning strategies, such as problem-based learning, flipping the classroom, and gamification, to engage learners and promote active learning. The educational escape room experience provides a way to engage and empower nursing students and assist in meeting learning objectives. This article describes an interactive educational escape activity for hematologic disorders.
Interaction Between Primary Tumor Resection, Primary Tumor Location, and Survival in Synchronous Metastatic Colorectal Cancer
Authors: van der Kruijssen Dave E.W. , Brouwer Nelleke P.M. , van der Kuil Auke J.S. , Verhoeven Rob H.A. , Elias Sjoerd G. , Vink Geraldine R. , Punt Cornelis J.A. , de Wilt Johannes H.W. , Koopman Miriam 
Published: April 2021

Objectives:
Location of the primary tumor has prognostic value and predicts the effect of certain therapeutics in synchronous metastatic colorectal cancer. We investigated whether the association between primary tumor resection (PTR) and overall survival (OS) also depends on tumor location.

Methods:
Data on synchronous metastatic colorectal cancer patients from the Netherlands Cancer Registry (n=16,106) and Surveillance, Epidemiology, and End Results (SEER) registry (n=19,584) were extracted. Cox models using time-varying covariates were implemented. Median OS for right-sided colon cancer (RCC), left-sided colon cancer, and rectal cancer was calculated using inverse probability weighting and a landmark point of 6 months after diagnosis as reference.

Results:
The association between PTR and OS was dependent on tumor location (P<0.05), with a higher median OS of upfront PTR versus upfront systemic therapy in Netherlands Cancer Registry (NCR) of 1.9 (95% confidence interval: 0.9-2.8), 4.3 (3.3-5.6), and 3.4 (0.6-7.6) months in RCC, left-sided colon cancer and rectal cancer, respectively. In SEER data, the difference was 6.0 (4.0-8.0), 8.0 (5.0-10.0), and 10.0 (7.0-13.0) months, respectively. Hazard plots indicate a higher hazard of death 2 to 3 months after PTR in RCC.

Conclusion:
Upfront PTR is associated with improved survival regardless of primary tumor location. Patients with RCC appear to have less benefit because of higher mortality during 2 to 3 months after PTR.
Generalized Multinucleate Cell Angiohistiocytoma: Histopathological and Immunohistochemical Analyses of 10 Lesions
Authors: Ross Casey L. , Chung Jina , Ross Nicholas A. , Lee Jason B. 
Published: April 2021

Abstract:
Multinucleate cell angiohistiocytoma (MCAH) is an uncommon fibrohistiocytic disorder that usually presents as a localized solitary papule or multiple grouped papules. Generalized presentation is very rare with less than 20 cases reported in the literature. In this article, we present histopathological and immunohistochemical studies of 10 lesions from a patient with generalized MCAH. In all lesions, the histopathological changes were confined to a discrete zone of the superficial dermis that consisted of (1) an increase in the number of capillary-sized vessels with thickened walls, (2) presence of oval to dendritic spindle cells and stellate hyperchromatic multinucleated cells, (3) fibrosis marked by compact collagen, (4) hypertrophy and hyperplasia of small nerve fibers, and (5) a moderately dense lymphocytic infiltrate. The entire population of the cellular component including the multinucleated cells stained for CD10, whereas a subpopulation of the mononuclear spindle cells stained for factor XIIIa and CD68. CD34 highlighted only the blood vessels. The results confirm that the multinucleated cells lack expression of CD68 and factor XIIIa and that CD10 may be used to highlight the entire cellular component. The rarely reported hypertrophy and hyperplasia of nerve fibers in MCAH may be a common finding as it was observed in all 10 lesions.
Visual Field Endpoints Based on Subgroups of Points May Be Useful in Glaucoma Clinical Trials: A Study With the Humphrey Field Analyzer and Compass Perimeter
Authors: Barkana Yaniv , Leshno Ari , Stern Ori , Singer Reut , Russ Hermann , Oddone Francesco , Lanzetta Paolo , Perdicchi Andrea , Johnson Chris A. , Garway-Heath David F. , Rossetti Luca M. , Skaat Alon 
Published: April 2021

Precis:
Visual field (VF) endpoints based on average deviation of specific subsets of points rather than all points may offer a more homogeneous data set without necessarily worsening test-retest variability and so may be useful in clinical trials.

Purpose:
The purpose of this study was to characterize the outcome measures encompassing particular subsets of VF points and compare them as obtained with Humphrey [Humphrey visual field analyser (HVF)] and Compass perimeters.

Methods:
Thirty patients with imaging-based glaucomatous neuropathy performed a pair of 24-2 tests with each of 2 perimeters. Nonweighted mean deviation (MD) was calculated for the whole field and separate vertical hemifields, and again after censoring of points with low sensitivity (MDc) and subsequently including only “abnormal” points with a total deviation probability of <5% (MDc5%) or <2% (MDc2%). Test-retest variability was assessed using Bland-Altman 95% limits of agreement (95%LoA).

Results:
For the whole field, using HVF, MD was −7.5±6.9 dB, MDc −3.6±2.8 dB, MDc5% −6.4±1.7 dB, and MDc2% −7.3±1.5 dB. With Compass the MD was −7.5±6.6, MDc −2.9±1.7 dB, MDc5% −6.3±1.5, and MDC2% −7.9±1.6. The respective 95%LoA were 5.5, 5.3, 4.6, and 5.6 with HVF, and 4.8, 3.7, 7.1, and 7.1 with Compass. The respective number of eligible points were 52, 42±12, 20±11, and 15±9 with HVF, and 52, 41.2±12.6, 10±7, and 7±5 with Compass. With both machines, SD and 95%LoA increased in hemifields compared with the total field, but this increase was mitigated after censoring.

Conclusion:
Restricting analysis to particular subsets of points of interest in the VF after censoring points with low sensitivity, as compared with using the familiar total field MD, can provide outcome measures with a broader range of MD, a markedly reduced SD and therefore more homogeneous data set, without necessarily worsening test-retest variability.
Diaphragm function in acute respiratory failure and the potential role of phrenic nerve stimulation
Authors: Reardon Peter M. , Wong Jenna , Fitzpatrick Aisling , Goligher Ewan C. 
Published: April 2021

Purpose of review
The aim of this review was to describe the risk factors for developing diaphragm dysfunction, discuss the monitoring techniques for diaphragm activity and function, and introduce potential strategies to incorporate diaphragm protection into conventional lung-protective mechanical ventilation strategies.

Recent findings
It is increasingly apparent that an approach that addresses diaphragm-protective ventilations goals is needed to optimize ventilator management and improve patient outcomes. Ventilator-induced diaphragm dysfunction (VIDD) is common and is associated with increased ICU length of stay, prolonged weaning and increased mortality. Over-assistance, under-assistance and patient-ventilator dyssynchrony may have important downstream clinical consequences related to VIDD. Numerous monitoring techniques are available to assess diaphragm function, including respiratory system pressures, oesophageal manometry, diaphragm ultrasound and electromyography. Novel techniques including phrenic nerve stimulation may facilitate the achievement of lung and diaphragm-protective goals for mechanical ventilation.

Summary
Diaphragm protection is an important consideration in optimizing ventilator management in patients with acute respiratory failure. The delicate balance between lung and diaphragm-protective goals is challenging. Phrenic nerve stimulation may be uniquely situated to achieve and balance these two commonly conflicting goals.
Latinx Family Perspectives on Social Needs Screening and Referral during Well-Child Visits
Authors: Spain Angeline K. , Monahan Emma K. , Alvarez Kiara , Finno-Velasquez Megan 
Published: April 2021

Abstract

Objective:
To understand Latinx parent perspectives on screening and referral approaches to identify social determinants of health and address social and material needs during well-child visits and to identify techniques that promote their engagement with these approaches.

Study Design:
We investigated parent perspectives and engagement with social needs screening and referral practices during well-child visits using focus group methodology.

Results:
We conducted 17 focus groups (n = 134 parents and other primary caregivers) with families receiving care at sites operated by eight pediatric primary care clinics. Adding social needs screening and referral shifted the social context of well-child visits for Latinx parents. Participants reported greater engagement with the practices where they perceived an emotional connection with nurses and other clinicians and cultural brokering to ensure their screening responses accurately reflected family needs and priorities. Participants highlighted the importance of having a personal patient–provider relationship as many preferred to discuss social needs outside of the exam room.

Clinical Implications:
Nurses and other clinicians play a critical role in establishing systems needed to systematically screen and refer families for social needs. Future policy development should consider implications for nursing practice and leadership in adopting these approaches.
Super-resolution structured illumination microscopy: past, present and future
Authors: Prakash Kirti , Diederich Benedict , Reichelt Stefanie , Heintzmann Rainer , Schermelleh Lothar 
Published: April 2021

Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware and software development and the use of commercial instruments to biological applications. This special issue aims to recap recent developments as well as outline future trends. In addition to SIM, we cover related topics such as complementary super-resolution microscopy techniques, computational imaging, visualization and image processing methods.This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.
Extended mechanical force measurements using structured illumination microscopy
Authors: Korobchevskaya Kseniya , Colin-York Huw , Barbieri Liliana , Fritzsche Marco 
Published: April 2021

Quantifying cell generated mechanical forces is key to furthering our understanding of mechanobiology. Traction force microscopy (TFM) is one of the most broadly applied force probing technologies, but its sensitivity is strictly dependent on the spatio-temporal resolution of the underlying imaging system. In previous works, it was demonstrated that increased sampling densities of cell derived forces permitted by super-resolution fluorescence imaging enhanced the sensitivity of the TFM method. However, these recent advances to TFM based on super-resolution techniques were limited to slow acquisition speeds and high illumination powers. Here, we present three novel TFM approaches that, in combination with total internal reflection, structured illumination microscopy and astigmatism, improve the spatial and temporal performance in either two-dimensional or three-dimensional mechanical force quantification, while maintaining low illumination powers. These three techniques can be straightforwardly implemented on a single optical set-up offering a powerful platform to provide new insights into the physiological force generation in a wide range of biological studies.
This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)'.
Attenuated alpha oscillations as an index of cortical hyperexcitability: Evidence from migraine patients
Authors: Fong Chun Yuen , Law Wai Him Crystal , Fahrenfort Johannes Jacobus , Braithwaite Jason J. , Mazaheri Ali 
Published: April 2021

AbstractAnomalous phantom visual perceptions coupled to an aversion to some visual patterns has been associated with aberrant cortical hyperexcitability in migraine patients. Previous literature has found fluctuations of alpha oscillation (8-14 Hz) over the visual cortex to be associated with the gating of the visual stream. In the current study, we examined whether alpha activity was differentially modulated in migraineurs in anticipation of an upcoming stimulus as well as post-stimulus periods. We used EEG to examine the brain activity in a group of 28 migraineurs (17 with aura/11 without) and 29 non-migraineurs and compared the modulations of alpha power in the pre/post-stimulus period relative to onset of stripped gratings of 3 spatial frequencies 0.5, 3, and 13 cycles per degree (cpd). Overall, we found that that migraineurs had significantly less alpha power prior to the onset of the stimulus relative to controls. Moreover, relative to the control group, migraineurs had significantly greater post-stimulus alpha suppression (i.e event-related desynchronization) induced by the 3 cpd grating at the 2nd half of the experiment, the stimulus most often reported to induce visual disturbances. These findings taken together provide strong support of the presence of elevated cortical excitability in the visual cortex of migraine sufferers. We speculate that cortical hyperexcitation could be the consequence of impaired perceptual learning driven by the dysfunction of GABAergic inhibitory mechanism.
Predicting a diagnosis of ankylosing spondylitis using primary care health records – a machine learning approach
Authors: Kennedy Jonathan , Kennedy Natasha , Cooksey Roxanne , Choy Ernest , Siebert Stefan , Rahman Muhammad , Brophy Sinead 
Published: April 2021

AbstractAnkylosing spondylitis is the second most common cause of inflammatory arthritis. However, a successful diagnosis can take a decade to confirm from symptom onset (via x-rays). The aim of this study was to use machine learning methods to develop a profile of the characteristics of people who are likely to be given a diagnosis of AS in future.The Secure Anonymised Information Linkage databank was used. Patients with ankylosing spondylitis were identified using their routine data and matched with controls who had no record of a diagnosis of ankylosing spondylitis or axial spondyloarthritis. Data was analysed separately for men and women. The model was developed using feature/variable selection and principal component analysis to develop decision trees. The decision tree with the highest average F value was selected and validated with a test dataset.The model for men indicated that lower back pain, uveitis, and NSAID use under age 20 is associated with AS development. The model for women showed an older age of symptom presentation compared to men with back pain and multiple pain relief medications. The models showed good prediction (positive predictive value 70%-80%) in test data but in the general population where prevalence is very low (0.09% of the population in this dataset) the positive predictive value would be very low (0.33%-0.25%).Machine learning can be used to help profile and understand the characteristics of people who will develop AS, and in test datasets with artificially high prevalence, will perform well. However, when applied to a general population with low prevalence rates, such as that in primary care, the positive predictive value for even the best model would be 1.4%. Multiple models may be needed to narrow down the population over time to improve the predictive value and therefore reduce the time to diagnosis of ankylosing spondylitis.
COVID-19 Vaccine Hesitancy among the Adult Population in Bangladesh: A Nationally Representative Cross-sectional Survey
Authors: Hossain Mohammad Bellal , Alam Md. Zakiul , Islam Md. Syful , Sultan Shafayat , Faysal Md. Mahir , Rima Sharmin , Hossain Md. Anwer , Mamun Abdullah Al 
Published: April 2021

ABSTRACTIntroductionThe study related to the COVID-19 vaccine hesitancy is scanty in the context of Bangladesh, despite the growing necessity of understanding the mass people’s vaccination-related behavior. Thus, the present study was conducted to assess the prevalence of the COVID-19 vaccine hesitancy and its associated factors in Bangladesh to fill the knowledge gap.MethodologyThis study adopted a cross-sectional study design to collect data from 1497 respondents using online (Google forms) and face-to-face interviews. We employed descriptive statistics and multiple hierarchical linear regression analysis.FindingsThe prevalence of vaccine hesitancy was 41.1%. Men had less hesitancy (β = -0.046, p = 0.030) than women. The Muslims (β = 0.057, p = 0.009) and the respondents living in the city corporation areas (β = 0.132, p <0.001) had more hesitancy. There was significant variation in vaccine hesitancy by administrative divisions (geographic regions). The vaccine hesitancy tended to decrease with increasing knowledge about the vaccine (β = -0.072, p=0.001) and the vaccination process (β= -0.058, p = 0.018). On the other hand, hesitancy increased with the increased negative attitudes towards vaccine (β = 0.291, p <0.001) and conspiracy beliefs towards the COVID-19 vaccine (β = 0.105, p=0.004). The perceived severity of the COVID-19 (β = -0.079, p=0.002) and perceived benefits of COVID-19 vaccination (β = -0.180, p=0.001) were negatively associated with hesitancy, while perceived barriers (β = 0.180, p <0.001) were positively associated. The participants were more hesitant to accept the vaccine from a specific manufacturer.ConclusionThis study emphasizes that negative attitudes and conspiracies towards the COVID-19 vaccine should be reduced through effective communications and contracting with additional vaccine manufacturers should be prioritized. The barriers like online registration for receiving the COVID-19 vaccination need to be removed, and initiatives like text message service using the mobile phone operator can be introduced.HighlightsAbout 41% of the respondents had had hesitancy to accept the COVID-19 vaccine.The hesitancy increased with negative attitudes about vaccines and conspiracy beliefs.Perceived barriers to receive the vaccine were increasing vaccine hesitancy.Perceived severity of the COVID-19 decreased the vaccine hesitancy.Perceived benefits of receiving the COVID-19 vaccine decreased the vaccine hesitancy.