bluuebunny/crossref_metadata_2025_split huggingface dataset:
100% renewable energy with pumped-hydro-energy storage in Nepal
Authors: Lohani Sunil Prasad , Blakers Andrew 
Published: May 2021

Abstract
A radical transformation of the global energy system is underway. Solar photovoltaics and wind now comprise three-quarters of the global net new electricity-generation-capacity additions because they are cheap. The deep renewable electrification of energy services including transport, heating and industry will allow solar and wind to largely eliminate fossil fuels over the next few decades. This paper demonstrates that Nepal will be able to achieve energy self-sufficiency during the twenty-first century. Nepal has good solar and moderate hydroelectric potential but has negligible wind- and fossil-energy resources. The solar potential is about 100 times larger than that required to support a 100% solar-energy system in which all Nepalese citizens enjoy a similar per-person energy consumption to developed countries, without the use of fossil fuels and without the environmental degradation resulting from damming Nepal’s Himalayan rivers. Nepal has vast low-cost off-river pumped hydro-energy-storage potential, thus eliminating the need for on-river hydro storage and moderating the need for large-scale batteries. Solar, with support from hydro and battery storage, is likely to be the primary route for renewable electrification and rapid growth of the Nepalese energy system.
Structural Connectivity Gradients of the Temporal Lobe Serve as Multiscale Axes of Brain Organization and Cortical Evolution
Authors: Vos de Wael Reinder , Royer Jessica , Tavakol Shahin , Wang Yezhou , Paquola Casey , Benkarim Oualid , Eichert Nicole , Larivière Sara , Xu Ting , Misic Bratislav , Smallwood Jonathan , Valk Sofie L , Bernhardt Boris C 
Published: May 2021

AbstractThe temporal lobe is implicated in higher cognitive processes and is one of the regions that underwent substantial reorganization during primate evolution. Its functions are instantiated, in part, by the complex layout of its structural connections. Here, we identified low-dimensional representations of structural connectivity variations in human temporal cortex and explored their microstructural underpinnings and associations to macroscale function. We identified three eigenmodes which described gradients in structural connectivity. These gradients reflected inter-regional variations in cortical microstructure derived from quantitative magnetic resonance imaging and postmortem histology. Gradient-informed models accurately predicted macroscale measures of temporal lobe function. Furthermore, the identified gradients aligned closely with established measures of functional reconfiguration and areal expansion between macaques and humans, highlighting their potential role in shaping temporal lobe function throughout primate evolution. Findings were replicated in several datasets. Our results provide robust evidence for three axes of structural connectivity in human temporal cortex with consistent microstructural underpinnings and contributions to large-scale brain network function.
Novel Canine Coronavirus Isolated from a Hospitalized Patient With Pneumonia in East Malaysia
Authors: Vlasova Anastasia N , Diaz Annika , Damtie Debasu , Xiu Leshan , Toh Teck-Hock , Lee Jeffrey Soon-Yit , Saif Linda J , Gray Gregory C 
Published: May 2021

AbstractBackgroundDuring the validation of a highly sensitive panspecies coronavirus (CoV) seminested reverse-transcription polymerase chain reaction (RT-PCR) assay, we found canine CoV (CCoV) RNA in nasopharyngeal swab samples from 8 of 301 patients (2.5%) hospitalized with pneumonia during 2017–2018 in Sarawak, Malaysia. Most patients were children living in rural areas with frequent exposure to domesticated animals and wildlife.MethodsSpecimens were further studied with universal and species-specific CoV and CCoV 1-step RT-PCR assays, and viral isolation was performed in A72 canine cells. Complete genome sequencing was conducted using the Sanger method.ResultsTwo of 8 specimens contained sufficient amounts of CCoVs as confirmed by less-sensitive single-step RT-PCR assays, and 1 specimen demonstrated cytopathic effects in A72 cells. Complete genome sequencing of the virus causing cytopathic effects identified it as a novel canine-feline recombinant alphacoronavirus (genotype II) that we named CCoV–human pneumonia (HuPn)–2018. Most of the CCoV-HuPn-2018 genome is more closely related to a CCoV TN-449, while its S gene shared significantly higher sequence identity with CCoV-UCD-1 (S1 domain) and a feline CoV WSU 79-1683 (S2 domain). CCoV-HuPn-2018 is unique for a 36-nucleotide (12–amino acid) deletion in the N protein and the presence of full-length and truncated 7b nonstructural protein, which may have clinical relevance.ConclusionsThis is the first report of a novel canine-feline recombinant alphacoronavirus isolated from a human patient with pneumonia. If confirmed as a pathogen, it may represent the eighth unique coronavirus known to cause disease in humans. Our findings underscore the public health threat of animal CoVs and a need to conduct better surveillance for them.
Neuroscientific therapies for atrial fibrillation
Authors: Hanna Peter , Buch Eric , Stavrakis Stavros , Meyer Christian , Tompkins John D , Ardell Jeffrey L , Shivkumar Kalyanam 
Published: May 2021

Abstract
The cardiac autonomic nervous system (ANS) plays an integral role in normal cardiac physiology as well as in disease states that cause cardiac arrhythmias. The cardiac ANS, comprised of a complex neural hierarchy in a nested series of interacting feedback loops, regulates atrial electrophysiology and is itself susceptible to remodelling by atrial rhythm. In light of the challenges of treating atrial fibrillation (AF) with conventional pharmacologic and myoablative techniques, increasingly interest has begun to focus on targeting the cardiac neuraxis for AF. Strong evidence from animal models and clinical patients demonstrates that parasympathetic and sympathetic activity within this neuraxis may trigger AF, and the ANS may either induce atrial remodelling or undergo remodelling itself to serve as a substrate for AF. Multiple nexus points within the cardiac neuraxis are therapeutic targets, and neuroablative and neuromodulatory therapies for AF include ganglionated plexus ablation, epicardial botulinum toxin injection, vagal nerve (tragus) stimulation, renal denervation, stellate ganglion block/resection, baroreceptor activation therapy, and spinal cord stimulation. Pre-clinical and clinical studies on these modalities have had promising results and are reviewed here.
An exploration of the relationship between recruitment communication and foraging in stingless bees
Authors: I’Anson Price Robbie , Segers Francisca , Berger Amelia , Nascimento Fabio S , Grüter Christoph 
Published: May 2021

Abstract
Social information is widely used in the animal kingdom and can be highly adaptive. In social insects, foragers can use social information to find food, avoid danger, or choose a new nest site. Copying others allows individuals to obtain information without having to sample the environment. When foragers communicate information they will often only advertise high-quality food sources, thereby filtering out less adaptive information. Stingless bees, a large pantropical group of highly eusocial bees, face intense inter- and intra-specific competition for limited resources, yet display disparate foraging strategies. Within the same environment there are species that communicate the location of food resources to nest-mates and species that do not. Our current understanding of why some species communicate foraging sites while others do not is limited. Studying freely foraging colonies of several co-existing stingless bee species in Brazil, we investigated if recruitment to specific food locations is linked to 1) the sugar content of forage, 2) the duration of foraging trips, and 3) the variation in activity of a colony from 1 day to another and the variation in activity in a species over a day. We found that, contrary to our expectations, species with recruitment communication did not return with higher quality forage than species that do not recruit nestmates. Furthermore, foragers from recruiting species did not have shorter foraging trip durations than those from weakly recruiting species. Given the intense inter- and intraspecific competition for resources in these environments, it may be that recruiting species favor food resources that can be monopolized by the colony rather than food sources that offer high-quality rewards.
More in, better out? Successful valve-in-valve procedure of an iatrogenic ventricular septal defect following transcatheter aortic valve replacement: a case report
Authors: Hönemann Klaus-Dieter , Hofmann Steffen , Ritter Frank , Mönnig Gerold 
Published: May 2021

Abstract

Background
A rare, but serious, complication following transcatheter aortic valve replacement (TAVR) is the occurrence of an iatrogenic ventricular septal defect (VSD).

Case summary
We describe a case of an 80-year-old female who was referred with severe aortic stenosis for TAVR. Following thorough evaluation, the heart team consensus was to proceed with implantation via a transapical approach of an ACURATE neo M 25 mm valve (Boston Scientific, Natick, MA, USA). The valve was deployed harnessing transoesophageal echocardiographic (TOE) guidance under rapid pacing with post-dilation. Directly afterwards a very high VSD close to the aortic annulus was detected. As the patient was haemodynamically stable, the procedure was ended. The next day another TOE revealed a shunt volume (left-to-right ventricle) between 50% and 60%. Because the defect was partly located between the stent struts of the ACURATE valve decision was made to fix this leakage with implantation of a further valve and we chose an EVOLUT Pro 29 mm (Medtronic Inc., Minneapolis, MN, USA). The valve-in-valve was implanted 2–3 mm below the lower edge of the first valve, more towards the left ventricular outflow tract (LVOT) with excellent result: VSD was reduced to a very small residual shunt without any hemodynamic relevance.

Discussion
We suggest that an iatrogenic VSD located near the annulus may be treated percutaneously in a bail-out situation with implantation of a second valve that should be implanted slightly more into the LVOT to cover the VSD.
A suicide attempt on a left ventricular assist device patient during COVID-19 pandemic: can we only blame the virus? A case report
Authors: Jiménez-Blanco Bravo Marta , Zamorano Gómez José Luis , del Prado Díaz Susana , Alonso Salinas Gonzalo Luis 
Published: May 2021

Abstract

Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has emerged as a new threat, not only to Health Care systems but also to citizen’s freedom of movement in many developed countries.

Case summary
We report a suicidal attempt in a destination therapy left ventricular assist device patient, potentially triggered by coronavirus disease 2019 (COVID-19) lockdown, highlighting the importance of regular and long-term psychological support for this vulnerable population.

Discussion
The psychological consequences of this pandemic, particularly in chronically ill patients, are yet to be defined.
Late diagnosed DiGeorge syndrome in a 44-year-old female: a rare cause for recurrent syncopes in adulthood—a case report
Authors: Isgandarova Khuraman , Molatta Stephan , Sommer Philipp 
Published: May 2021

Abstract

Background
DiGeorge syndrome, also known as ‘CATCH 22’, is the most common deletion in humans and is one of the velocardiofacial syndromes. It is characterized by a specific facial phenotype, and structural and functional abnormalities in the cardiac and endocrine systems. One form of endocrine system dysfunction is hypocalcaemia, which causes arrhythmic events and can result in a transient loss of consciousness. We present a case highlighting the late diagnosis of DiGeorge syndrome in a patient with recurrent episodes of syncope due to suspected arrhythmic events secondary to hypocalcaemia.

Case summary
A 44-year-old woman was referred for further investigation of recurrent syncope episodes and documented transient QT-prolongation with hypocalcaemia. Previous detailed cardiological examination, including invasive procedures such as coronary angiography and cardiac magnetic resonance tomography, was unremarkable. Slight characteristic facial dysmorphia and transient hypocalcaemia were strongly suggestive of DiGeorge syndrome. The diagnosis was confirmed by genetic testing. Calcium substitution was initiated and no recurrent episodes of syncope or arrhythmic events were reported during 12 months of follow-up.

Discussion
Clinical presentation and time of manifestation of the DiGeorge syndrome varies widely depending on the mutation expression extent. An atypical disease course may delay the diagnosis and appropriate management of affected patients. In this case, confirmation of the diagnosis allowed the initiation of appropriate treatment, reducing the risk for further events. Given that syncope and arrhythmia can be the first and only manifestation of late-onset DiGeorge syndrome, specialists in adult cardiology need to be aware of this presentation.
Current trends in reduction or elimination of the aortic impulse during stent-graft deployment and balloon moulding during thoracic endovascular aortic repair
Authors: Gottardi Roman , Wyss Thomas R , van den Berg Jos C , Rylski Bartosz , Berger Tim , Schmidli Juerg , Czerny Martin ,   Aortic Impulse in TEVAR Group, Luca Bertoglio , Arminder Jassar , Oliver Bernecker , Eric Verhoeven , Santi Trimarchi , Luca Di Marco , Theodoros Kratimenos , Patrick Ruchhat , Chris Probst , Mario Lescan , Yutaka Okita , Manfred Cejna , Christian Reeps , Nikolaos Tsilimparis , Maximiliam Luehr , Christoph Nienaber , Gabriele Piffaretti , Øyvind Jakobsen , Alessandro Della Corte , Kenji Minatoya , Florian Wolf , Toru Kuratani , Martina Fink , Robert Ma , Marco Di Eusanio , Gabriel Weiss , Ourania Preventza , Kay-Hyun Park , Ahmed Koshty , Michael Jacobs , Ernst Weigang , Martin Storck , Robin Heijmen , Peter-Lukas Haldenwang , Rolf Alexander Janosi , Robert Seelos , Alexander Hyhlik-Dürr , Luís Mendes Pedro , Alexander Zimmermann , Stephen Cheng , Joseph Shalhoub , Inge Fourneau , Joost van Herwaarden , Yehuda Wolf , Zoltan Szeberin , Berard Xavier , Julia Dumfarth 
Published: May 2021

Abstract

OBJECTIVES
A survey was performed to evaluate the methods used for reduction or elimination of the aortic impulse (REAI) to facilitate precise stent graft placement and balloon moulding during thoracic endovascular aortic repair (TEVAR).

METHODS
A total of 127 physicians (1 per hospital) were contacted and asked to fill out a short, comprehensive questionnaire on an internet-based platform.

RESULTS
Fifty physicians (39.4%) responded and completed the survey. Routine use of REAI for stent graft deployment is most frequently used in the ascending aorta and less frequently in the aortic arch and the descending aorta (86.4% vs 69.4% vs 56%). Some physicians based the decision of whether to use REAI on the type of stent graft in the respective location (13.6% vs 24.5% vs 24.0%). Stent-graft deployment without REAI, irrespective of the type of stent graft used, was never done in the ascending aorta (0.0%), in 3 centres in the aortic arch (6.1%) and in 10 centres in the descending aorta (20%). The REAI method most frequently used was dependent on the aortic segment (ascending aorta vs aortic arch vs descending aorta) rapid right ventricular pacing (90.9% vs 59.2% vs 28.0%), followed by pharmacological blood pressure reduction (13.6% vs 53.1% vs 64.0%) and venous inflow occlusion (13.6% vs 14.3% vs 4.0%), respectively. Tip capture and non-occlusive deployment systems were frequently quoted as reasons for not using REAI.

CONCLUSIONS
REAI is the fundament for TEVAR in all thoracic aortic segments, with a decline in usage from proximal (ascending) to distal (descending). Rapid right ventricular pacing is the preferred REAI method used in TEVAR. Most procedures are performed with the patient under general anaesthesia. The types of stent grafts and moulding balloons used have an impact on the use or non-use of REAI.
Treatment of a massive pseudoaneurysm originating from the ascending aorta with an off-the-shelf stent graft
Authors: Gottardi Roman , Buchholz Stefan , Hegazy Yasser , Sodian Ralf 
Published: May 2021

Abstract
A 73-year-old patient who underwent an emergency coronary bypass surgery in our institution and who required postoperative extracorporeal membrane oxygenation support in December 2019 presented in August 2020 with a rapidly growing subxiphoidal, pulsating swelling. A computed tomography scan revealed a massive mediastinal pseudoaneurysm originating from an 8-mm Dacron graft that was sutured to the ascending aorta during the index surgery for arterial extracorporeal membrane oxygenation cannulation. Due to the location and extent of the pseudoaneurysm, an open surgical revision was deemed high risk. Because no bypass conduit originated from the ascending aorta, we decided to occlude the entry of the pseudoaneurysm with a stent graft. Also, urgency did not allow for the manufacturing of a custom-made device, so an off-the-shelf stent graft had to be implanted. Currently, the only off-the-shelf thoracic stent graft with a length suitable for the ascending aorta (&lt;7 cm) is the Medtronic Valiant Navion prosthesis, of which 2 prostheses (37 mm × 52 mm; covered seal) were successfully implanted to exclude the pseudoaneurysm. A follow-up computed tomography scan performed 4 months postoperatively showed no perfusion and regression of the pseudoaneurysm. With the growing number of reports describing stent graft placement in the ascending aorta, more off-the-shelf stent grafts suitable for the ascending aorta are desirable.
Insect High Five™ cell line development using site-specific flipase recombination technology
Authors: Dias Mafalda M , Vidigal João , Sequeira Daniela P , Alves Paula M , Teixeira Ana P , Roldão António 
Published: May 2021

Abstract
Insect Trichoplusia ni High Five™ (Hi5) cells have been widely explored for production of heterologous proteins, traditionally mostly using the lytic baculovirus expression vector system (BEVS), and more recently using virus-free transient gene expression systems. Stable expression in such host cells would circumvent the drawbacks associated with both systems when it comes to scale-up and implementation of more efficient high-cell density process modes for the manufacturing of biologics. In this study, we combined Flipase (Flp) recombinase-mediated cassette exchange (RMCE) with fluorescence-activated cell sorting (FACS) for generating a stable master clonal Hi5 cell line with the flexibility to express single or multiple proteins of interest from a tagged genomic locus. The 3-step protocol herein implemented consisted of (i) introducing the RMCE docking cassette into the cell genome by random integration followed by selection in Hygromycin B and FACS (Hi5-tagging population), (ii) eliminating cells tagged in loci with low recombination efficiency by transfecting the tagging population with an eGFP-containing target cassette followed by selection in G418 and FACS (Hi5-RMCE population), and (iii) isolation of pure eGFP-expressing cells by FACS and expansion to suspension cultures (Hi5-RMCE master clone). Exchangeability of the locus in the master clone was demonstrated in small-scale suspension cultures by replacing the target cassette by one containing a single protein (i.e., iCherry, as an intracellular protein model) or two proteins (i.e., influenza HA and M1 for virus-like particles production, as an extracellular protein model). Overall, the stable insect Hi5 cell platform herein assembled has the potential to assist and accelerate biologics development.
Immigration Background and the Widowhood Effect on Mortality
Authors: Caputo Jennifer , Li Peng , Kühn Mine , Brønnum-Hansen Henrik , Oksuzyan Anna 
Published: May 2021

AbstractObjectivesWidowhood is a stressful life event with one of the most profound negative effects on health and longevity. Immigrant populations are growing and aging throughout Western nations, and marginalization and cultural differences may make some immigrants especially vulnerable to the stressors of widowhood. However, studies have yet to systematically explore whether the widowhood effect differs between immigrant and native-born individuals.MethodsUsing Danish population register data from 1980 to 2014, this study assesses whether the relationship between widowhood and mortality differs between immigrants from 10 countries and native-born Danes aged 50 and older at 0–2, 3–5, and 6 and more years post-widowhood.ResultsWe find that immigrant men are at higher risk of dying in the first 2 years after experiencing widowhood than Danish-born men, but these mortality differences dissipate over longer periods. Immigrant women have a higher risk of having died 3 and more years after a spouse’s death than Danish women. Patterns vary further by country of origin.DiscussionThe results suggest that some immigrants may suffer more from widowhood than native-born individuals, giving insight into how immigration background may influence the health effects of negative life events. They also underscore the potential vulnerabilities of aging immigrant populations to stressors encountered in older age.
Caudal Spine Morphology and Puncture Performance of Two Coastal Stingrays
Authors: Shea-Vantine Caitlin S , Galloway Katherine A , Ingle Danielle N , Porter Marianne E , Kajiura Stephen M 
Published: May 2021

Abstract
A diagnostic characteristic of stingrays in the family Dasyatidae is the presence of a defensive, partially serrated spine located on the tail. We assessed the contribution of caudal spine morphology on puncture and withdrawal performance from two congeneric, co-occurring stingrays, the Atlantic stingray, Hypanus sabinus, and the bluntnose stingray, Hypanus say. Spines exhibited a high degree of morphological variability. Stingray spines were serrated along 50.8% (H. sabinus) or 62.3% (H. say) of their length. Hypanus say had a greater number of serrations along each side of the spine (30.4) compared with H. sabinus (20.7) but the pitch did not differ between species. We quantified spine puncture and withdrawal forces using porcine skin as a model for human skin. Puncture and withdrawal forces did not differ significantly between species, or within H. say, but withdrawal force was greater than puncture force for H. sabinus. We incorporated micro-computed tomography scanning to quantify tissue mineral density and found that for both species, the shaft of the spine was more heavily mineralized than the base, and midway (50%) along the length of the spine was more heavily mineralized than the tip. The mineralization variability along the spine shaft may create a stiff structure that can fracture once embedded within the target tissue and act as an effective predator deterrent.