bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute measures of upper thermal and hypoxia tolerance are not reliable predictors of mortality following environmental challenges in rainbow trout (Oncorhynchus mykiss)
Authors: Strowbridge Nicholas , Northrup Sara L , Earhart Madison L , Blanchard Tessa S , Schulte Patricia M 
Published: December 2021

Abstract
Anthropogenic climate change threatens freshwater biodiversity and poses a challenge for fisheries management, as fish will increasingly be exposed to episodes of high temperature and low oxygen (hypoxia). Here, we examine the extent of variation in tolerance of acute exposure to these stressors within and among five strains of rainbow trout (Oncorhynchus mykiss) currently being used or under consideration for use in stocking programmes in British Columbia, Canada. We used incipient lethal oxygen saturation (ILOS) as an index of acute hypoxia tolerance, critical thermal maximum (CTmax) as an index of acute upper thermal tolerance and mortality following these two acute exposure trials to assess the relative resilience of individuals and strains to climate change-relevant stressors. We measured tolerance across two brood years and two life stages (fry and yearling), using a highly replicated design with hundreds of individuals per strain and life stage. There was substantial within-strain variation in CTmax and ILOS, but differences among strains, although statistically significant, were small. In contrast, there were large differences in post-trial mortality among strains, ranging from less than 2% mortality in the most resilient strain to 55% mortality in the least resilient. There was a statistically significant, but weak, correlation between CTmax and ILOS at both life stages for some strains, with thermally tolerant individuals tending to be hypoxia tolerant. These data indicate that alternative metrics of tolerance may result in different conclusions regarding resilience to climate change stressors, which has important implications for stocking and management decisions for fish conservation in a changing climate.
Does Higher Education Have Liberalizing or Inoculating Effects? A Panel Study of Anti-Immigrant Sentiment before, during, and after the European Migration Crisis
Authors: Velásquez Paolo , Eger Maureen A 
Published: December 2021

Abstract
Previous research has consistently shown a negative correlation between education and anti-immigrant sentiment. This association is most pronounced when distinguishing between adults with higher education and those without a tertiary degree. Yet it remains unclear whether educational attainment actually matters for attitudes, mainly due to a lack of longitudinal studies. This article investigates the so-called liberalizing effect of education on adults’ attitudes towards immigrants by taking into account individual, regional, and period effects. Using 12 waves of the Norwegian Citizen Panel (2013–2020) combined with contextual data from Statistics Norway, we assess the effects of: (i) educational attainment at the individual level; (ii) the expansion of higher education at the regional level; and (iii) higher education during a time of social upheaval. Results from multilevel cross-classified, repeated measurement models show that within-individual and within-county changes in educational attainment have a small but liberalizing effect on attitudes. Furthermore, individuals with at least 3–4 years of university education do not react as strongly to the highly salient European migration crisis than those with lower levels of education. This finding suggests that higher education inhibits perceptions of threat that may manifest during ‘big events’ such as a dramatic increase in asylum seeking. We interpret these novel results as evidence of an inoculating effect, in that higher education protects individuals against whatever instinct exists to react strongly during such crises.
Stimulation of Dissimilatory Sulfate Reduction in Response to Sulfate in Microcosm Incubations from Two Contrasting Temperate Peatlands near Ithaca, NY, USA
Authors: St James Andrew R , Richardson Ruth E 
Published: December 2021

Abstract
Peatlands are responsible for over half of wetland methane emissions, yet major uncertainties remain regarding carbon flow, especially when increased availability of electron acceptors stimulate competing physiologies. We used microcosm incubations to study the effects of sulfate on microorganisms in two temperate peatlands, one bog and one fen. Three different electron donor treatments were used (13C-acetate, 13C-formate, and a mixture of 12C short-chain fatty acids) to elucidate the responses of sulfate-reducing bacteria (SRB) and methanogens to sulfate stimulation. Methane production was measured and metagenomic sequencing was performed, with only the heavy DNA fraction sequenced from treatments receiving 13C electron donors. Our data demonstrate stimulation of dissimilatory sulfate reduction in both sites, with contrasting community responses. In McLean Bog (MB), hydrogenotrophic Deltaproteobacteria and acetotrophic Peptococcaceae lineages of SRB were stimulated, as were lineages with unclassified dissimilatory sulfite reductases. In Michigan Hollow Fen (MHF), there was little stimulation of Peptococcaceae populations, and a small stimulation of Deltaproteobacteria SRB populations only in the presence of formate as electron donor. Sulfate stimulated an increase in relative abundance of reads for both oxidative and reductive sulfite reductases, suggesting stimulation of an internal sulfur cycle. Together, these data indicate a stimulation of SRB activity in response to sulfate in both sites, with a stronger growth response in MB than MHF. This study provides valuable insights into microbial community responses to sulfate in temperate peatlands and is an important first step to understanding how SRB and methanogens compete to regulate carbon flow in these systems.
Nonconventional yeasts to produce aroma compounds by using agri-food waste materials
Authors: Karaalioğlu Onur , Yüceer Yonca Karagül 
Published: December 2021

ABSTRACT
Nowadays, biotechnological applications are emphasized to ensure sustainable development by reutilizing waste materials to prevent ecological problems and to produce or recover compounds that may have positive effects on health. Yeasts are fascinating microorganisms that play a key role in several traditional and innovative processes. Although Saccharomyces is the most important genus of yeasts, and they are major producers of biotechnological products worldwide, a variety of other yeast genera and species than Saccharomyces that are called ‘non-Saccharomyces’ or ‘nonconventional’ yeasts also have important potential for use in biotechnological applications. Some of the nonconventional yeast strains offer a unique potential for biotechnological applications to produce valuable secondary metabolites due to their characteristics of surviving and growing in such extreme conditions, e.g. wide substrate range, rapid growth, thermotolerance, etc. In this review, we aimed to summarize potential biotechnological applications of some nonconventional yeasts (Kluyveromyces spp., Yarrowia spp., Pichia spp., Candida spp., etc.) to produce industrially important aroma compounds (phenylethyl alcohol, phenylethyl acetate, isobutyl acetate, diacetyl, etc.) by reutilizing agri-food waste materials in order to prevent ecological problems and to produce or recover compounds that may have positive effects on health.
The Myth of Responsibility: Colonial Cruelties and Silence in German Political Discourse
Authors: Augsten Pauline , Glassner Sebastian , Rall Jenni 
Published: December 2021

Abstract
Germany is considered a role model for dealing with past mass atrocities. In particular, the social reappraisal of the Holocaust is emblematic of this. However, when considering the genocide on the Herero and Nama in present-day Namibia, it is puzzling that an official recognition was only pronounced after almost 120 years, in May 2021. For a long time, silence surrounded this colonial cruelty in German political discourse. Although the discourse on German responsibility toward Namibia emerged after the end of World War II, it initially appeared detached from the genocide. That silence on colonial atrocities is to be considered a cruelty itself. Studies on silence have been expanding and becoming richer. Building on these works, the paper sets two goals: First, it advances the theorization of silence by producing a new typology, which is then integrated into discourse-bound identity theory. Second, it applies this theory to the analysis of the silencing and later acknowledging of the genocide on the Herero and Nama by German political elites. To this end, Bundestag debates, official documents, and statements by relevant political actors are analyzed in the period from 1980 to 2021. The results reveal the dynamics between hegemonic and counter-hegemonic discursive formations, how those are shifting in a period of 40 years, and what role silence plays in it. Beyond our emphasis on the genocide on the Herero and Nama, our findings might benefit future studies as the approach proposed in this paper can make silence a tangible research object for global studies.
Mitochondria metabolomics reveals a role of β-nicotinamide mononucleotide metabolism in mitochondrial DNA replication
Authors: Nomiyama Tomoko , Setoyama Daiki , Yasukawa Takehiro , Kang Dongchon 
Published: December 2021

Abstract
Mitochondrial DNA (mtDNA) replication is tightly regulated and necessary for cellular homeostasis; however, its relationship with mitochondrial metabolism remains unclear. Advances in metabolomics integrated with the rapid isolation of mitochondria will allow for remarkable progress in analyzing mitochondrial metabolism. Here, we propose a novel methodology for mitochondria-targeted metabolomics, which employs a quick isolation procedure using a hemolytic toxin from Streptococcus pyogenes streptolysin O (SLO). SLO isolation of mitochondria from cultured HEK293 cells is time- and labor-saving for simultaneous multi-sample processing and has been applied to various other cell lines in this study. Furthermore, our method can detect the time-dependent reduction in mitochondrial ATP in response to a glycolytic inhibitor 2-deoxyglucose, indicating the suitability to prepare metabolite analysis–competent mitochondria. Using this methodology, we searched for specific mitochondrial metabolites associated with mtDNA replication activation, and nucleotides and NAD+ were identified to be prominently altered. Most notably, treatment of β-nicotinamide mononucleotide (β-NMN), a precursor of NAD+, to HEK293 cells activated and improved the rate of mtDNA replication by increasing nucleotides in mitochondria and decreasing their degradation products: nucleosides. Our results suggest that β-NMN metabolism plays a role in supporting mtDNA replication by maintaining the nucleotide pool balance in the mitochondria.
On the professional authority of quality engineers and the gaps in their epistemic and organizational authority
Authors: Anker Sharon , Lurie Yotam 
Published: December 2021

ABSTRACT
The authority of quality engineers as a profession is a contested issue that relates both to the occupation’s internal regulation and to the professional status of quality engineers within the organizations for which they work. In this article, we examine the professional authority of quality engineers from both these perspectives. The issue is addressed first through a conceptual framework, followed by a presentation of an empirical study in which seven experienced quality professionals were interviewed and completed questionnaires. We focus on the situation in Israel as a case study, but our insights are relevant to quality engineers throughout the world. We demonstrate limitations with regard to both (1) the epistemic authority (expertise) of quality engineers vis-à-vis their status as a professional association and (2) the organizational authority of quality engineers vis-à-vis their organizational role as quality managers. These limitations can be attributed to the occupation’s status as a semi-profession; the level of expertise required is not fixed or uniform; the authority of a quality engineer varies from one organizational structure to another. In addition, individual quality engineers are accorded different levels of influence, usually at the discretion of their employing organizations. For these reasons, the expertise of quality engineers as a professional group remains an open issue.
Ionizing radiation alters organoid forming potential and replenishment rate in a dose/dose-rate dependent manner
Authors: Fujimichi Yuki , Otsuka Kensuke , Tomita Masanori , Iwasaki Toshiyasu 
Published: December 2021

Abstract
Intestinal organoids are an in vitro cultured tissue model generated from intestinal stem cells, and they contain a mixture of epithelial cell types. We previously established an efficient ‘one cell/well’ sorting method, and defined organoid-forming potential (OFP) as a useful index to evaluate the stemness of individual cells. In this study, we assessed the response to radiation dose and dose-rate by measuring both OFP and the percentage of stem cells in the crypts. After high-dose-rate (HDR, 0.5 Gy/min) irradiation in vivo, the percentage of stem cells in the harvested crypt cells decreased, and the replenishment of cycling stem cells originating from dormant cells was enhanced, but OFP increased in cells irradiated with a total dose of &gt;1 Gy. In contrast, at a total dose of 0.1 Gy the percentage of stem cells reduced slightly, but neither replenishment rate nor OFP changed. Furthermore, the response to 1 Gy of low-dose-rate (LDR) irradiation was similar to the response to 0.1 Gy HDR irradiation. These results suggest that 0.1 Gy HDR irradiation or 1 Gy LDR irradiation does not alter stemness. Additionally, the OFP increase in the colon in response to irradiation was smaller than that in the duodenum, similar to the percentage of stem cells. Understanding the differences in the response of stem cells between the colon and the duodenum to radiation is important to clarify the mechanisms underlying the development of radiation-associated intestinal cancers.
Dynamics around non-spherical symmetric bodies – I. The case of a spherical body with mass anomaly
Authors: Madeira G , Giuliatti Winter S M , Ribeiro T , Winter O C 
Published: December 2021

ABSTRACT
The space missions designed to visit small bodies of the Solar system boosted the study of the dynamics around non-spherical bodies. In this vein, we study the dynamics around a class of objects classified by us as non-spherical symmetric bodies, including contact binaries, triaxial ellipsoids, and spherical bodies with a mass anomaly, among others. In this work, we address the results for a body with a mass anomaly. We apply the pendulum model to obtain the width of the spin–orbit resonances raised by non-asymmetric gravitational terms of the central object. The Poincaré surface of section technique is adopted to confront our analytical results and to study the system’s dynamics by varying the parameters of the central object. We verify the existence of two distinct regions around an object with a mass anomaly: a chaotic inner region that extends beyond the corotation radius and a stable outer region. In the latter, we identify structures remarkably similar to those of the classical restrict and planar three-body problem in the Poincaré surface of sections, including asymmetric periodic orbits associated with 1:1+p resonances. We apply our results to a Chariklo with a mass anomaly, obtaining that Chariklo rings are probably related to first kind periodic orbits and not with 1:3 spin–orbit resonance, as proposed in the literature. We believe that our work presents the first tools for studying mass anomaly systems.
Neuropsychiatric symptoms associated with the COVID-19 and its potential nervous system infection mechanism: the role of imaging in the study
Authors: Du Yanyao , Zhao Wei , Du Lei , Liu Jun 
Published: December 2021

AbstractThe epidemic of coronavirus disease 2019 (COVID-19) has broken the normal spread mode of respiratory viruses, namely, mainly spread in winter, resulting in over 230 million confirmed cases of COVID-19. Many studies have shown that severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can affect the nervous system by varying degrees. In this review, we look at the acute neuropsychiatric impacts of COVID-19 patients, including acute ischemic stroke, encephalitis, acute necrotizing encephalopathy, dysosmia, and epilepsy, as well as the long-term neuropsychiatric sequelae of COVID-19 survivors: mental disorder and neurodegenerative diseases. In particular, this review discusses long-term changes in brain structure and function associated with COVID-19 infection. We believe that the traditional imaging sequences are important in the acute phase, while the nontraditional imaging sequences are more meaningful for the detection of long-term neuropsychiatric sequelae. These long-term follow-up changes in structure and function may also help us understand the causes of neuropsychiatric symptoms in COVID-19 survivors. Finally, we review previous studies and discuss some potential mechanisms of SARS-CoV-2 infection in the nervous system. Continuous focus on neuropsychiatric sequelae and a comprehensive understanding of the long-term impacts of the virus to the nervous system is significant for formulating effective sequelae prevention and management strategies, and may provide important clues for nervous system damage in future public health crises.
From Politics as Vocation to Politics as Business: Populist Social Performance and Narrative Fusion in Trump Rallies
Authors: Karakaya Yagmur , Edgell Penny 
Published: December 2021

Abstract
Populist movements seek to bolster the power of “the people” and undermine elites. In the United States a businessman, Donald Trump, has convinced a significant portion of the population that he is a man of the people. We answer three inter-related questions about what may be Trump’s biggest “win”: the transformation of populist discourse for a new century. How does Trump embed himself inside his followers’ own deep story? How does he fuse their story with a tale of American restoration? And how does he delegitimize politics as a vocation and valorize politics as business? Drawing on a systematic analysis of Trump/MAGA rallies held in four different regions from 2015 to 2021, we analyze how Trump used his performance to crystalize a distinctly American style of populism. We focus on the cultural accomplishment of his performance, particularly the creation of a business-friendly rhetoric that leverages popular cultural idioms to legitimate politics not as a vocation, but as a business. We find that Trump uses the popular idioms of standup comedy and competitive sports culture. This performance contributed to his 2016 win, yet framing politics as a game to be won runs the risk of reducing deliberative democratic process to election-night outcomes, makes political parties into opposing teams, and divides voters into winners and losers.
“I Don’t Have Much Money, but I Have a Lot of Friends”: How Poor Older Latinxs Find Social Support in Peer Friendship Networks
Authors: Plasencia Melanie Z 
Published: December 2021

AbstractEven though older Latinxs face some of the greatest economic precarity of any demographic group in the nation, little research has focused on this group and how they survive, despite having limited economic means and access to government support. Drawing on two years of ethnographic research in an urban, Latinx community on the East Coast and on 72 in-depth interviews, this study addresses this knowledge gap by focusing on the role of peer friendship networks in the lives of low-income, foreign-born Latinx older adults. I show that peer friendships have the unique ability to prioritize and affirm their identities as Latinxs and older adults and provide returns in the form of medical, economic, and emotional support. Peers often facilitate transportation to medical appointments, provide critical information about medicines and health insurance, and try to uplift one another emotionally, especially when family support is lacking. Understanding the role these networks play in the lives of our most vulnerable has implications for our understanding of aging, poverty, and policy.
The Exposome and Toxicology: A Win–Win Collaboration
Authors: Barouki Robert , Audouze Karine , Becker Christel , Blaha Ludek , Coumoul Xavier , Karakitsios Spyros , Klanova Jana , Miller Gary W , Price Elliott J , Sarigiannis Denis 
Published: December 2021

AbstractThe development of the exposome concept has been one of the hallmarks of environmental and health research for the last decade. The exposome encompasses the life course environmental exposures including lifestyle factors from the prenatal period onwards. It has inspired many research programs and is expected to influence environmental and health research, practices, and policies. Yet, the links bridging toxicology and the exposome concept have not been well developed. In this review, we describe how the exposome framework can interface with and influence the field of toxicology, as well as how the field of toxicology can help advance the exposome field by providing the needed mechanistic understanding of the exposome impacts on health. Indeed, exposome-informed toxicology is expected to emphasize several orientations including (1) developing approaches integrating multiple stressors, in particular chemical mixtures, as well as the interaction of chemicals with other stressors, (2) using mechanistic frameworks such as the adverse outcome pathways to link the different stressors with toxicity outcomes, (3) characterizing the mechanistic basis of long-term effects by distinguishing different patterns of exposures and further exploring the environment-DNA interface through genetic and epigenetic studies, and (4) improving the links between environmental and human health, in particular through a stronger connection between alterations in our ecosystems and human toxicology. The exposome concept provides the linkage between the complex environment and contemporary mechanistic toxicology. What toxicology can bring to exposome characterization is a needed framework for mechanistic understanding and regulatory outcomes in risk assessment.