bluuebunny/crossref_metadata_2025_split huggingface dataset:
The LINC01410/miR‐122‐5p/NDRG3 axis is involved in the proliferation and migration of osteosarcoma cells
Authors: Ma Weiguo , Zhao Xin , Xue Ning , Gao Yun , Xu Qingxia 
Published: February 2021

AbstractPurposeIt is generally accepted that long noncoding RNAs (lncRNAs) function as vital regulators of tumor development and progression. Long intergenic non‐coding RNA 1410 (LINC01410) is a newly discovered lncRNA, and its role in osteosarcoma (OS) is yet to be determined.Materials and methodsThe expression of LINC01410, microRNA‐122‐5p (miR‐122‐5p), and N‐myc downstream‐regulated gene 3 (NDRG3) in OS tissues was determined using reverse transcription‐quantitative PCR. Interactions between LINC01410, miR‐122‐5p, and NDRG3 were predicted and verified using bioinformatics tools and luciferase assays. Cell proliferation, migration, and invasion were detected using cell counting Kit‐8 and Transwell assays.ResultsLINC01410 was overexpressed in OS tissues. Furthermore, it was confirmed that LINC01410 facilitated OS cell proliferation and migration. Our studies also showed that LINC01410 binds to miR‐122‐5p, and miR‐122‐5p binds to NDRG3. Finally, we observed that LINC01410 knockdown inhibited the proliferation, invasion, and migration of OS cells. Knockdown of LINC01410 resulted in the upregulation of miR‐122‐5p and downregulation of NDRG3.ConclusionOur results demonstrated that the LINC01410/miR‐122‐5p/NDRG3 axis is involved in the progression of OS.
Grappling With Ideas: Adolescent Writers in a Rural African American Community
Authors: Spence Lucy K. , Walker Robert M. 
Published: February 2021

AbstractAn African American teacher and students engaged in meaningful reading and writing that included African American literature. These literacy experiences allowed the development of cultural models through exploring identity, questioning dominant narratives, and building capacity for literary analysis. This study shows how meaningful literacy pedagogy for African American students can be implemented during the school day despite the pressures of a heavily constrained curriculum.
Double‐flow convolutional neural network for rapid large field of view Fourier ptychographic reconstruction
Authors: Sun Minglu , Shao Lina , Zhu Youqiang , Zhang Yuxi , Wang Shaoxin , Wang Yukun , Diao Zhihui , Li Dayu , Mu Quanquan , Xuan Li 
Published: February 2021

AbstractFourier ptychographic microscopy is a promising imaging technique which can circumvent the space‐bandwidth product of the system and achieve a reconstruction result with wide field‐of‐view (FOV), high‐resolution and quantitative phase information. However, traditional iterative‐based methods typically require multiple times to get convergence, and due to the wave vector deviation in different areas, the millimeter‐level full‐FOV cannot be well reconstructed once and typically required to be separated into several portions with sufficient overlaps and reconstructed separately, which makes traditional methods suffer from long reconstruction time for a large‐FOV (of the order of minutes) and limits the application in real‐time large‐FOV monitoring of live sample in vitro. Here we propose a novel deep‐learning based method called DFNN which can be used in place of traditional iterative‐based methods to increase the quality of single large‐FOV reconstruction and reducing the processing time from 167.5 to 0.1125 second. In addition, we demonstrate that by training based on the simulation dataset with high‐entropy property (Opt. Express 28, 24 152 [2020]), DFNN could has fine generalizability and little dependence on the morphological features of samples. The superior robustness of DFNN against noise is also demonstrated in both simulation and experiment. Furthermore, our model shows more robustness against the wave vector deviation. Therefore, we could achieve better results at the edge areas of a single large‐FOV reconstruction. Our method demonstrates a promising way to perform real‐time single large‐FOV reconstructions and provides further possibilities for real‐time large‐FOV monitoring of live samples with sub‐cellular resolution.
Line field confocal optical coherence tomography: An adjunctive tool in the diagnosis of autoimmune bullous diseases
Authors: Tognetti Linda , Cinotti Elisa , Suppa Mariano , Guazzo Raffaella , Habougit Cyril , Santi Francesco , Diet Gwendoline , Fontaine Margot , Berot Vincent , Monnier Jilliana , Pianigiani Elisa , del Marmol Véronique , Malvehy Joseph , Perrot Jean‐Luc , Rubegni Pietro 
Published: February 2021

AbstractAutoimmune bullous diseases (AIBDs) still represent a considerable a source of morbidity and mortality: early identification of a specific AIBD is often difficult due to overlapping clinical and/or laboratory features and time‐consuming invasive laboratory tests. We aimed to investigate the potential role of a new imaging technology, line‐field confocal optical coherence tomography (LC‐OCT), in the non‐invasive diagnosis of AIBDs. LC‐OCT was performed at lesional, perilesional and contralateral healthy sites in 30 patients, before histology and direct immunofluorescence. LC‐OCT examination was able to identify the level of split (subcorneal/suprabasal/subepidermal/sublamina densa), to provide detailed images of the bulla roof morphology and content (eg, erythrocytes/acantholytic cells/polymorphonucleates). Areas of intra/subepidermal detachment were also detected also at clinically normal perilesional skin sites. LC‐OCT can support physicians, real time and at bed‐site, in the differential diagnosis of various AIBDs and their mimickers. Moreover, it can be used for the identification of subclinical lesions and therapy tapering.
Investigation of the effect of clinically relevant interferents on glucose monitoring using near‐infrared spectroscopy
Authors: Fuglerud Silje Skeide , Ellingsen Reinold , Aksnes Astrid , Hjelme Dag Roar 
Published: February 2021

AbstractNear infrared spectroscopy (NIR) is a promising technique for continuous blood glucose monitoring for diabetic patients. Four interferents, at physiological concentrations, were introduced to study how the glucose predictions varied with a standard multivariate calibration model. Lactate and ethanol were found to interfere strongly with the glucose predictions unless they were included in the calibration models. Lactate was mistaken for glucose and gave erroneously high glucose predictions, with a dose response of 0.46 mM/mM. The presence of ethanol resulted in too low glucose predictions, with a dose response of −0.43 mM/mM. Acetaminophen, a known interferent in the glucose monitoring devices used for diabetes management today, was not found to be an interferent in NIR spectroscopy, nor was caffeine. Thus, interferents that may appear in high concentrations, such as ethanol and lactate, must be included in the calibration or model building of future NIR‐based glucose measurement devices for diabetes monitoring.
Understanding the binding mechanism of succinic acid against phospholipase A2 from bee venom
Authors: Nidamarthi H. V. Kutumbarao , Choudhury Manisha , Velmurugan Devadasan 
Published: February 2021

AbstractPhospholipase A2 (PLA2) is responsible for the release of fatty acids from glycerophospholipids. PLA2 is commonly found in mammalian tissues. It is also found in venom from different animals ranging from insects, arachnid, and snakes. The release of arachidonic acid in large amount results in inflammation and pain. Identification of compounds that can inhibit the activity of PLA2 is of large scientific and medicinal interest as these compounds can act as antidotes toward snake bites and bee stings. Among the different compounds that have been tested for inhibition of PLA2, a secondary metabolite succinic acid is identified to inhibit PLA2 activity. The inhibition was analyzed using an in vitro PLA2 inhibition assay and isothermal titration calorimetry (ITC) studies. The molecular mechanism of the mode of inhibition was studied using molecular docking and simulation studies.
Modulating NF‐κB, MAPK, and PI3K/AKT signaling by ergothioneine attenuates iron overload‐induced hepatocellular injury in rats
Authors: Salama Samir A. , Omar Hany A. 
Published: February 2021

AbstractThe liver is highly susceptible to iron overload‐evoked oxidative injury. Ergothioneine is a thio‐histidine amino acid that has exhibited strong antioxidant and metal chelating activities. This study aimed at exploring the potential modulating effects of ergothioneine on iron‐triggered liver injury. The results showed that ergothioneine inhibited iron‐evoked inflammation and apoptosis as demonstrated by a significant reduction in tumor necrosis factor‐α and interleukin‐6 levels and in caspase‐3 activity. Ergothioneine significantly improved liver cell survival as indicated by modulating phosphatidylinositol 3‑kinase/protein kinase B signaling. Consistent with reduced necrotic cell death, ergothioneine diminished the iron‐evoked histopathological changes and decreased serum activity of the liver enzymes. Mechanistically, ergothioneine reduced nuclear translocation of nuclear factor kappa B p65 and modulated p38 mitogen‐activated protein kinase/c‐Fos signaling. In addition, it enhanced the liver tissue antioxidant potential and curbed hepatic iron load. Together, these results point out the modulatory effects of ergothioneine on iron‐evoked liver cell injury that are possibly mediated via anti‐inflammatory, antioxidant, and possible iron chelation capabilities.
Cytotoxicity, anti‐microbial studies of M(II)‐dithiocarbamate complexes, and molecular docking study against SARS COV2 RNA‐dependent RNA polymerase
Authors: Al‐Janabi Ahmed S. M. , Saleh Abdulrahman M. , Hatshan Mohammad R. 
Published: February 2021

AbstractTen transition metal dithiocarbamate (DTC)complexes of the type [M(κ2‐Et2DT)2] (1–5), and [M(κ2‐PyDT)2] (6–10) (where M = Co, Ni, Cu, Pd, and Pt; Et2DT = diethyl dithiocarbamate; PyDT = pyrrolidine dithiocarbamate) were synthesized and characterized by different methods. The dithiocarbamate acted as bidentate chelating ligands to afford a tetrahedral complexes with Co(II) ion and square planner with other transition metal ions. The dithiocarbamate complexes showed good activity against the pathogen bacteria species. The results showed the Pt‐dithiocarbamate complexes are more active against all the tested bacteria than the Pd‐dithiocarbamate complex. The dithiocarbamate complexes displayed the maximum inhibition zone against E. coli bacteria, whereas the lowest activity of the dithiocarbamate against Salmonella typhimurium bacteria. The cytotoxicity of the Pd(II) and Pt(II) complexes was screened against the MCF‐7 breast cancer cell line and the complexes showed moderate activity compared with the cis‐platin. The results indicated that the MCF7 cells treated with 500 μg\ml of ligands and Pd(II) and Pt(II) complexes after 24 hr exposure showed intercellular space and dead cells. Finally, molecular docking studies were carried out to examine the binding mode of the synthesized compounds against the proposed target; SARS COV2 RNA‐dependent RNA polymerase.
Reverse culture shock, distress symptoms and psychological well‐being of fresh foreign degree holders in Pakistan
Authors: Akhtar Mubeen , Kamal Anila , Imtiaz Sara , Hayee Aliya A. 
Published: February 2021

AbstractThis study explored to what extent does reverse culture shock predict the level of anxiety, depression, and psychological well‐being among fresh foreign degree holders in Pakistan, in addition to finding out the relationship between the study variables. Participants consisted of 124 fresh foreign degree holders (109 men and 15 women) who were recruited through purposive and snowball sampling techniques. Reverse Culture Shock Scale, Major Depression Inventory, Beck Anxiety Inventory, and WHO‐5 Well‐being Index were used to measure study variables. Results show that reverse culture shock is a strong predictor of psychological well‐being explaining 23% variance in well‐being scores of fresh foreign degree holders. As hypothesized, it has positive associations with distress symptoms including anxiety and depression, whereas, a negative correlation was found with psychological well‐being and the age of fresh foreign degree holders. However, contrary to the hypothesis, reverse culture shock has no association with time passed since arrival back home and duration of stay abroad.
Identification of small extracellular vesicle subtypes in follicular fluid: Insights into the function and miRNA profiles
Authors: Wang Xiaomei , Meng Kai , Wang Hengqin , Wang Ying , Zhao Yunqi , Kang Jian , Zhang Yong , Quan Fusheng 
Published: February 2021

AbstractThe study of small extracellular vesicles (sEVs) heterogeneity is one of the main problems that must be solved, and the different sEV subtypes in follicular fluid are still unclear, limiting our understanding of their function. This study first separated sEV subtypes from follicular fluid using differential ultracentrifugation combined with iodixanol density gradient flotation and then evaluated their miRNA profile and effects on the proliferation and apoptosis of granulosa cells (GCs). We also performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of potential target genes of differentially expressed miRNAs (DEMs) and KEGG analysis of potential target genes of non‐DEMs. Low‐density sEVs (sEV_F6) were enriched in TSG101, while high‐density sEVs (sEV_F8) were enriched in CD63. The miRNA profiles of sEV_F6 and sEV_F8 were heterogeneous, and the differential signaling pathways were mainly related to the adhesion and hypoxic stress pathways, while the same signaling pathways were mainly related to cell proliferation, apoptosis, cell cycle, and autophagy pathways. In addition, the highly expressed miRNAs in both subtypes were mainly related to cell proliferation and apoptosis. Both subtypes transferred their miRNAs into GCs and promoted the proliferation ability of the GCs and inhibited their apoptosis. The results showed for the first time that there are different subtypes of sEVs in follicular fluid and that the miRNA profiles of subtypes are heterogeneous.
ATAD3A stabilizes GRP78 to suppress ER stress for acquired chemoresistance in colorectal cancer
Authors: Huang Kevin Chih‐Yang , Chiang Shu‐Fen , Yang Pei‐Chen , Ke Tao‐Wei , Chen Tsung‐Wei , Lin Chen‐Yu , Chang Hsin‐Yu , Chen William Tzu‐Liang , Chao Kun‐San Clifford 
Published: February 2021

AbstractAAA domain containing 3A (ATAD3A) is a nucleus‐encoded mitochondrial protein with vital function in communication between endoplasmic reticulum (ER) and mitochondria which is participated in cancer metastasis. Here we show that elevated ATAD3A expression is clinically associated with poor 5‐year disease‐free survival in patients with colorectal cancer (CRC), especially high‐risk CRC patients who received adjuvant chemotherapy. Our results indicated ATAD3A is significantly upregulated to reduce chemotherapy‐induced cancer cell death. We found that knockdown of ATAD3A leads to dysregulation in protein processing for inducing ER stress by RNA sequencing (RNA‐seq). In response to chemotherapy‐induced ER stress, ATAD3A interacts with elevated GRP78 protein to assist protein folding and alleviate ER stress for cancer cell survival. This reduction of ER stress leads to reduce the surface exposure of calreticulin, which is the initiator of immunogenic cell death and antitumor immunity. However, silencing of ATAD3A enhances cell death, triggers the feasibility of chemotherapy‐induced ER stress for antitumor immunity, increases infiltration of T lymphocytes and delays tumor regrowth in vitro and in vivo. Clinically, CRC patients with less ATAD3A have high density of CD45+ intratumoral infiltrating lymphocytes (TILs) and memory CD45RO+ TILs. Taken together, our results suggest that pharmacologic targeting to ATAD3A might be a potential therapeutic strategy to enhance antitumor immunity for CRC patients who received adjuvant chemotherapy.
Characterization of the microbial community in a biotrickling filter treating a complex mixture of gaseous compounds causing odor nuisance
Authors: Aguirre Alberto , Gentina Juan C , Malhautier Luc , Fanlo Jean‐Louis , Vergara‐Fernandez Alberto , Scott Felipe , Aroca Germán 
Published: February 2021

AbstractBACKGROUNDIndustrial gaseous emissions causing odor nuisance has become a major environmental issue in many countries. Biotrickling filters are suitable for treating mixtures of gaseous compounds with high removal efficiencies. The aim of this work was to determine the vertical distribution of microbial communities established in a biotrickling filter treating a mixture of gaseous compounds causing odor nuisance, and to correlate its performance with the spatial and temporal distribution of inoculated bacteria.RESULTSIn the long‐term operation, at day 538, the removal efficiencies were 100% for ammonia and H2S, 73% for methanol, 63% for trimethylamine, 41% for dimethylsulphide, and 32% for 2,5‐dimethylpirazyne; the elimination capacities were 1.5 [g NH3 m−3bed h−1], 0.7 [g H2S m−3bed h−1], 0.7 [g MeOH m−3bed h−1], 3.3 [g TMA m−3bed h−1], 0.21 [g DMS m−3bed h−1], and 0.6 [g 2,5‐DMP m−3bed h−1]. A stratification pattern of pollutants degradation along the biotrickling filter (BTF) was identified, where most of the removal of NH3, TMA, and H2S occurred at the lower level of the column, the removal of methanol and 2,5‐dimethylpyrazine occurred at low and medium levels, and the removal of dimethylsulphide occurred mainly at the upper levels. High‐throughput DNA sequencing analysis showed a colonization by bacteria genera different from the inoculated and a stratification of these bacteria along the BTF.CONCLUSIONThere is stratification in the biofilm of a biotrickling filter during its long‐term operation, and a relationship between the stratification of the microbial communities and the removal of the complex mixture of gases was identified. © 2021 Society of Chemical Industry
A case of immune checkpoint inhibitor‐associated gastroenteritis detected by ultrasonography
Authors: Omotehara Satomi , Nishida Mutsumi , Yamanashi Kana , Sakurai Kensuke , Katsurada Takehiko , Komatsu Yoshito , Shimizu Ai , Shibuya Hitoshi , Shinagawa Naofumi , Sugita Junichi , Teshima Takanori 
Published: February 2021

AbstractWhile immune checkpoint inhibitors (ICIs) have antitumor effects, they also have characteristic side effects, including colitis. However, gastritis has rarely been reported. We report a case of a patient with lung adenocarcinoma who presented with epigastric pain and diarrhea following pembrolizumab administration. Sonography of the abdomen demonstrated diffuse, although mild, gastric wall thickening (mainly in the submucosa), as well as a slight decrease in echogenicity throughout the gastric wall. While the mucosal surface was relatively smooth, color Doppler examination showed increased vascularity. Esophagogastroduodenoscopy and pathological examination confirmed the diagnosis of ICI‐related gastroenteritis.
Reflective writing in dental education to improve critical thinking and learning: A systematic review
Authors: Woldt Janet L. , Nenad Monica W. 
Published: February 2021

AbstractThe Commission on Dental Accreditations states that “graduates must be competent in the use of critical thinking and problem‐solving.” With this in mind, dental education programs continually strive to enhance and deepen these skills by incorporating effective instructional strategies into the curriculum. To do this, predoctoral dental education has used a variety of techniques including problem‐based learning, OSCEs, and standardized patient activities. Another technique, while less popular but potentially more effective if done well, is reflective writing. This study aimed to assess the effect of reflective writing on students’ critical thinking skills and learning in dental education programs. Two database searches conducted between 2000 and 2019 resulted in 317 articles after the initial screening. Ultimately, 13 articles met inclusion criteria. The following recurring factors were identified for inclusion in dental education reflective writing initiative: reflective writing interventions, reflective writing instructions, writing prompts, evaluation frameworks, and negative perceptions of reflective writing. In answering the key aim of this review, 12 of the 13 studies determined that reflective writing had a positive impact on students’ critical thinking, judgment, and/or learning. Key findings included positive effects on student growth in reflection, learning through reflective writing, reflection skills, self‐assessment, critical thinking, clinical reasoning, problem solving, and motivation to change after evaluated experiences. The review indicated that dental education programs, which implemented reflective writing as an assessment tool within the curriculum as a means of developing and deepening critical thinking skills and learning were by and large successful in this effort.
Structural and ultrastructural characterization of the palatine epithelium of the Guinea pig: A new record of telocytes in the oral cavity
Authors: Tomiate André N. , Barbosa Gabriela K. , Rocha Lara C. , Vasconcelos Bruno G. , de Almeida Sonia R. Y. , Miglino Maria A. , Watanabe Ii‐sei , Ciena Adriano P. 
Published: February 2021

AbstractThe morphology of the oral cavity of mammals relates to diet, habitat, and function. The palate is an important region with adaptations for oral somatosensation and mechanical loads due to the pressure of the tongue with food. The research aimed to describe the structural and ultrastructural characteristics of the epithelium and the connective tissue cores of the guinea pig palate using macroscopic, light microscopy, scanning electron microscopy, and transmission electron microscopy analysis. The hard palate had conical and filiform papillae, and the soft palate had open salivary ducts. After the removal of the epithelium, the connective tissue cores revealed thin filaments and laminar projections in the hard palate, and opening ducts were evidenced in the soft palate. The palatine epithelium was keratinized and organized by layers, lamellated corpuscles were found in lamina propria of the hard palate. In contrast, the soft palate had glands clusters associated with nerve fibers, and in both regions were identified telocytes. We concluded that the hard palate presented conical and filiform papillae that differ from other mammals. Besides, it is a new description of the connective tissue cores morphology and the first record of the telocytes in this anatomical region for mammals.