bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apolipoprotein E genotyping variations and their influence on cognitive outcome among idiopathic generalized epileptic patients
Authors: El-Salhy Nancy , Eissa Noura R. , Ragab Salma , Abd-Allah Gamil M. , Sayed Sara M. 
Published: December 2024

Background/aim
Epilepsy is a brain disorder affecting individuals of all ages. It is one of the most common neurological disorders, affecting about 50 million people globally. The study objective was to study the different genotypes of Apolipoprotein E (APOE) gene among adult idiopathic generalized epileptic patients and to assess their influence on cognitive functions of those patients.

Patients and methods
Forty adult patients with idiopathic generalized epilepsy (IGE) and 20 healthy controls were enrolled in this study from Kafrelsheikh Governorate, Egypt. Patients were recruited from the Neurology Department at Kafrelsheikh University Hospital. Neuropsychological evaluation was conducted to all participants. Genotyping of the APOE gene polymorphisms (rs429358 and rs7412) was then carried out using both restriction fragments length polymorphism and Sanger sequencing to evaluate the correlation between the genotype and cognitive functions of patients with IGE.

Results
Most of the patients and controls showed the wild type genotype ε3/ε3 of both polymorphisms of APOE gene; rs429358 and rs7412. Only six patients and one control had different genotypes other than the wild type. The study showed that a highly significant association (P<0.001) between IGE and cognitive impairment especially attention, memory, and fluency. Meanwhile, no statistically significant differences were found in distribution of Addenbrooke score and parameters according to APOE genotype distribution in patients’ group, while in controls there was no significant difference in distribution of attention and visuospatial processing parameters according to genotype distribution. Yet, there was a significant increase in Addenbrooke, memory, fluency, and language processing in ε3/ε3 genotype compared to ε3/ε4 genotype, (P=0.000, 0.007, 0.016, and 0.000, respectively).

Conclusion
This study supports the evidence of a distinct cognitive profile in patients with epilepsy regardless the APOE gene genotype.
In silico study of Bacillus spB201 amylase through protein sequence inspection
Authors: Ben Mabrouk Sameh , Ben Hadj Hmida Bouthaina , Boukthir Ikram , Cherif Slim 
Published: December 2024

Background/aim
Bioinformatics is the automatic processing of biological information, and it is a helpful tool to study the structure-function relationship of various proteins. Here, it was used in the in silico study of an AmyB201 from Bacillus strain, named AmyB201 to describe its origin and properties, which could help us to improve the performance of this enzyme through mutagenesis.

Materials and methods
The protein sequence of AmyB201 was purchased from NCBI data Base. The Signal P and the Protparam were used to determine the properties of AmyB201. In addition, programs like Swiss-model, Phyre2 and PyMOL were used to generate and manipulate the AmyB201 models.

Results
Comparaison study of Amy B201using Blast program showed an identity of 94% and 91% with amylases from Bacillus spUS586 and Bacillus spUS572, respectively. Subsequently, the analysis of the sequence by the Signal P. program revealed the presence of a signal peptide, which confirms the extracellular nature of the enzyme. Furthermore, the examination of the AmyB201 sequence by Espript showed that it has the same secondary structure with amylases from Bacillus spUS586 and Bacillus spUS572, with the exception of a few differences that could explain the specificity of each enzyme. In addition, the inspection of the 3D models showed the presence of three typical domains of amylases, namely the domains: A, B, C. Using these structures, we have been able to explain some properties of AmyB201.

Conclusion
This study was able to describe the origin of some properties of AmyB201, and could help us to improve the performance of this enzyme through mutagenesis.
Evaluation of Predictors of Response to Ovulation Induction Using Letrozole in Women with Polycystic Ovary Syndrome: A Prospective Cohort Study
Authors: Tholiya Shanti , Kumari Archana , Mahey Reeta , Kalaivani Mani , Sati Hem Chandra , Sharma Mona , Jayraj Aarthi K. , Malhotra Neena 
Published: December 2024

ABSTRACT

Background:
Ovulation induction (OI) in patients with polycystic ovary syndrome (PCOS) remains challenging, and several biomarkers have been evaluated for their ability to predict ovulation. The predictive ability of candidate biomarkers, particularly with letrozole-based therapy in infertile PCOS women, remains inconclusive as it is yet to be evaluated in a prospective study.

Aim:
To assess the role of anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), luteinising hormone (LH)/FSH ratio, testosterone and free androgen index (FAI) as predictors of ovarian response to letrozole-based OI therapy during OI cycles in infertile women with PCOS from North India.

Settings and Design:
A prospective cohort study was conducted in a tertiary care hospital in north India.

Materials and Methods:
The study enrolled 80 infertile women with PCOS, diagnosed according to the Rotterdam criteria. OI was conducted using letrozole with or without human menopausal gonadotropin. Baseline endocrine and metabolic parameters, including serum AMH, FSH, LH, testosterone and FAI levels, were measured using ELISA or chemiluminescence methods on day 2 of the menstrual cycle. Follicular response to OI was monitored by transvaginal ultrasonography.

Statistical Analysis Used:
Descriptive and inferential statistical analyses were conducted, including Mann–Whitney, Kruskal–Wallis, Independent t-test, analysis of variance, Fisher’s exact test and receiver operating characteristic curve analysis. Data were processed using Microsoft Excel and analysed with SPSS software, version 25.0. P < 0.05 was considered statistically significant.

Results:
Of 80 women enrolled, 74 responded to letrozole-based OI, while six were non-responders. Body mass index (BMI), serum testosterone and pre-treatment AMH levels significantly correlated with follicular response, with higher values linked to reduced responsiveness. The likelihood ratio+ (95% confidence interval) was 3.32 (2.45–5.06) for AMH, 1.97 (1.03–3.78) for BMI and 1.93 (1.22–3.08) for testosterone. The odds ratio for AMH was 2.88 (1.01–8.21) and 1.25 (1.02–1.53) for BMI. An AMH cut-off of ≤16.43 ng/mL predicted ovarian response with an AUC of 0.88.

Conclusions:
Pre-treatment AMH levels, along with BMI and serum testosterone, are significant predictors of ovarian response to letrozole-based OI in infertile women with PCOS.
Eosinophilic Granuloma of Occipital Bone with Extradural Hematoma: A Case Report
Authors: Munnangi Veda , Jacob Sruthi , Rangasami Rajeswaran 
Published: December 2024

Abstract

Background:
Eosinophilic granuloma (EG) is a benign localized form of Langerhans cell histiocytosis (LCH), which commonly occurs in bones. Although EG can occur in skull bones, the occurrence of extradural hemorrhage (EDH) in a case of EG is very rare.

Objective:
We present a case of 8-year-old girl who was diagnosed to have EG in occipital bone with EDH based on radiological and histopathological investigations.

Materials and Methods:
We describe a case of 8-year-old girl who presented with swelling over the back of her head since 4 months with no other complaints. Computed tomography (CT) and magnetic resonance imaging (MRI) studies showed a lytic lesion in occipital bone with soft-tissue component and posterior fossa EDH. The patient underwent surgical excision of the lesion and a biopsy confirmed LCH.

Conclusions:
EDH is a neurosurgical emergency usually secondary to trauma. However, EG is an extremely rare nontraumatic cause of EDH.
Avançar o ensino de História das Américas: dados, práticas e desafios contemporâneos
Authors: Cerri Luis , Dalmás Carine , Conceição Juliana Pirola da 
Published: December 2024

O texto aborda o ensino de História nas Américas, destacando a diversidade curricular e os desafios enfrentados nas instituições de ensino. Na primeira parte, é enfatizada a importância do conhecimento histórico sobre a América Latina e a necessidade de transformar a situação atual do ensino, por meio de atividades de pesquisa que conectem a realidade local e regional a outros contextos latino-americanos. Na segunda parte, analisa-se a diversidade curricular da História nos distintos países da América Latina, evidenciando a necessidade de uma abordagem mais contextualizada e inclusiva. Por fim, a terceira avalia comparativamente a aprendizagem histórica e o interesse no estudo da História dos outros países por parte dos estudantes do Brasil, Argentina, México e Chile. As principais conclusões incluem a interconexão entre a luta pelo ensino de História e a defesa da escola pública, a necessidade de inclusão de diversas perspectivas, a crítica às diretrizes curriculares que enfatizam rivalidades, a importância da pesquisa para entender e melhorar o ensino, e as intervenções em políticas públicas educacionais para avançar o ensino de História das Américas.
Interpretando os sujeitos históricos: o uso do RPG no Ensino de História das Américas e suas possibilidades didáticas
Authors: Firmo dos Santos Alexandre 
Published: December 2024

A proposta deste artigo é apresentar e explicar a viabilidade do uso do RPG nas aulas de História, mais precisamente, nos conteúdos que envolvem as Américas. Para tanto, nos deteremos no caso em que os conceitos do RPG foram “adaptados” ao conteúdo específico desse campo de estudo, a saber: A colonização das Américas, com ênfase na primeira viagem de Cristóvão Colombo (1492). Lançamos mão de uma bibliografia específica sobre o tema a fim de explanar melhor o objeto pesquisado. Dessa forma, observou-se, que o RPG permite uma interação entre aqueles que participam e contribuem com a jogabilidade.
Self-Translation and the Translator’s (In)visibility in Nabokov’s Pnin (1957)
Authors: Russo Michele 
Published: December 2024

Nabokovian scholarship has consistently emphasised the plurilingual aspects in Nabokov’s oeuvre. His American novels frequently include numerous sentences in Russian and French, which are often translated or discussed from a metalinguistic angle by the author. A remarkable example in this regard is the autobiography Speak, Memory. An Autobiography Revisited (1966), which conjures up various places where Nabokov spent different periods of his life, along with the languages overlapping in his European memoirs.
Considering that the coexistence of foreign terms and expressions is common in Nabokov’s works, this article aims to analyse the linguistic architecture and aspects of self-translation in one of his American novels, Pnin. While this study dwells on the numerous grammatical and phonetic inaccuracies that feature in the protagonist’s dialogues, it also means to investigate the issue pertaining to the translator’s (in)visibility in the text. The analysis will be carried out in light of Venuti’s (2018) theories, which foreground the crucial yet often overlooked role of the translator, particularly in contexts where translation is domesticated. By quoting various examples of self-translated expressions from the novel, as well as grammatical and phonetic mistakes, the article traces the linguistic borders in the story, shedding light on the work of the translator, whose presence becomes discernible through the foreignizing effect generated by the numerous non-English words, mistakes and inaccuracies. The work thus engages with the issue of Nabokov’s (in)visibility in the story, since the translator’s presence is not always detectable as the storyline progresses.
The alternation of domestication and foreignization, linguistic adaptation and estrangement, is infused with the writer’s investigation into his linguistic past which, in Cronin’s (2013, 19) words, sheds light on the “historical sense” of the languages employed. The exploration of Nabokov’s linguistic past, expressed through numerous foreignisms, paves the way for an analysis of the writer’s double, thereby providing a more nuanced illustration of Nabokov’s linguistic transition.
OVERVIEW OF CLASS-I TRIPARTITE MOTIF (TRIM) PROTEINS SPECIFICALLY TRIM67
Authors: Panhwar S. N. , Huang C. , Luo Q. , Yu X. , Jia L. , Liu W. , Chen Z. 
Published: December 2024

The TRIM family is a group of genes expressed during embryogenesis. This review emphasized data on Class-I TRIM genes, including TRIM1, TRIM9, TRIM36, TRIM46, TRIM67, TRIM18, and TRIM76. This article will discuss the structure and function of these genes and their roles in various physiological processes such as embryonic development, and immune regulation. Class-I TRIM proteins develop as E3 ubiquitin ligases and work with E2 ubiquitin-conjugating enzymes. TRIM9 is expressed in the stem cells of mice and linked to various neuronal functions and neurological diseases. TRIM18 is found in microtubules and cellular filaments. TRIM36 is associated with neuro-pathological mutations, while TRIM46 controls microtubule organisation during axon formation. TRIM67 plays a significant role in neuritis outgrowth, lipid phosphate phosphatase, and colorectal and lung carcinomas. It also involves cell proliferation and induces morphological changes like neuronal differentiation. TRIM67 is important in Plasticity-related gene-1 (PRG-1). Deletion of prog-1 in mice results in epileptic seizures, indicating the significance of TRIM67 in brain development and growth. TRIM76 is associated with cardiac diseases. Further identification and characterisation of novel TRIM members will provide new insights into the functions of these essential proteins in health and disease.
A Dynamic Trapdoor Redactable Blockchain Schemes
Authors: Li Shuqiao , Hu Xiaoming , Liu Yan , Bai Shuangjie 
Published: December 2024

The permanent storage of blockchain data exposes certain security risks in practical applications, necessitating the ability to edit data in specific scenarios. The chameleon hash function, as a trapdoor one-way hash function, has emerged as a key technology for achieving redactable blockchains. However, existing redactable blockchain schemes based on chameleon hash function face security vulnerabilities related to trapdoor misuse and leakage. This paper proposes a dynamic trapdoor redactable blockchain schemes, utilizing a dynamic trapdoor chameleon hash (DTCH) that allows for the dynamic update of trapdoors while overcoming the inherent immutability of blockchain, thus reducing the risk of malicious nodes retaining the trapdoor long-term. The proposed scheme employs controllable randomness, where the trapdoor key automatically updates after a certain period or upon use. In this scenario, authorized nodes cannot successfully find hash collisions with old keys, effectively revoking their authorization. Consequently, only users with valid trapdoor keys can modify the data on the chain. Relevant security tests and experimental results indicate that the proposed scheme offers advantages in security and maintains good efficiency at various stages, achieving higher security guarantees with minimal computational cost.

Received: 27 September 2024 | Revised: 5 November 2024 | Accepted: 4 December 2024

Conflicts of Interest
The authors declare that they have no conflicts of interest to this work.

Data Availability Statement
Data sharing is not applicable to this article as no new data were created or analyzed in this study.

Author Contribution Statement
Shuqiao Li: Conceptualization, Methodology, Software, Validation, Formal analysis, Resources, Data curation, Writing – original draft, Writing – review & editing, Visualization, Supervision, Project administration. Xiaoming Hu: Conceptualization, Methodology, Resources, Writing – original draft, Writing – review & editing, Visualization, Supervision, Project administration. Yan Liu: Formal analysis, Resources, Writing – review & editing, Visualization, Supervision. Shuangjie Bai: Validation, Formal analysis, Writing – review & editing, Visualization.
In vitro Insights into Prospect of Cnestis ferruginea Pulp Extract as an Antimicrobial Agent
Authors: Ajala Lawrence , Ogunlowo Oladejo , Okafor Mathew , Ezem Stella , Ogunlowo Olayemi , Adeleke Adebayo 
Published: December 2024

Sensitivity study of Cnestis ferruginea pulp extract with respect to the reference commercial antibiotics was tested against four bacteria (Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis, and Klebsiella pneumoniae) and three fungi (Trichoderum rubrum, Aspergillus niger, and Candida albicans) strains isolated in man with a view to elucidating the inhibitory potency of the plant pulp’ extract using an agar well diffusion assay. The investigation discovered that the extract had 71% inhibitory activities against the microbial isolates tested either in sterilized or non-sterilized form and in the two different concentrations examined, which compared favourably with reference antibiotics used. The optimal performance of the pulp extract against the test organisms could be due to the presence of the metabolites such as alkaloids, flavonoids, steroids, saponins, and polyphenols found in ample form in the extract. This study has revealed the possible utilization of Cnestis ferrugineain the treatment of wound, urinary infections and in the management of oral-related infections.
Исследование различных типов переменных объектов с помощью данных TESS
Authors: Тарасенков А.Н. 
Published: December 2024

Космический аппарат Transiting Exoplanet Survey Satellite (TESS) получил большое количество высокоточных фото- метрических данных, которые можно использовать для решения огромного количества астрономических задач. Но точности и полноты данных, предоставляемых открытым архивом данных TESS, не всегда достаточно для их реше- ния. Поэтому, мы представляем простой, но мощный конвейер на основе пакетов Lightkurve и Astrobase для обработки и анализа сырых данных TESS. Мы также приводим краткий обзор наших исследований, в которых мы используем данные TESS, проанализированные с помощью этого конвейера. Мы проводим программу поиска и подтверждения переменных звезд с использованием архивов кадров малых фотометрических телескопов Астрономического инсти- тута им. П. К. Штернберга (ГАИШ) и Института астрономии РАН (ИНАСАН), в которой используем высокоточную фотометрию TESS и обзора ZTF. Мы также проводим картирование хромосферно-активных звезд, используя кривые блеска, полученные из необработанных данных TESS с помощью нашего конвейера. Также мы приводим фотометрию карликовых новых и других транзиентных астрономических объектов на основе нашей обработки данных TESS.
Transiting Exoplanet Survey Satellite (TESS) spacecraft obtained a large amount of high-precision photometric data, which can be used to solve a huge number of astronomical problems. But the accuracy and completeness of the data provided by the TESS open data archive is not always enough to solve them. So we present simple but powerful pipeline based on Lightkurve and astrobase, for raw TESS data reduction and analysis. We also present a short overview of our research in which we use TESS data, analysed using this pipeline. We are conducting a program to search and confirm variable stars using archives of frames from small photometric telescopes of Sternberg Astronomical Institute (SAI) and Institute of Astronomy of the Russian Academy of Sciences (INASAN), in which we use high-precision photometry of TESS and Zwicky Transient Facility (ZTF). We also conduct mapping chromospherically active stars using light curves extracted from raw TESS data by our pipeline. We also present photometry of dwarf novae and other transient astronomical objects based on our processing of TESS data.
Сегментированный гамма-спектрометр для регистрации транзиентных гамма-источников
Authors: Мкртчян А.А. , Позаненко А.С. , Минаев П.Ю. 
Published: December 2024

Гамма-всплески — это одни из самых ярких транзиентных электромагнитных явлений во Вселенной. Несмотря на многолетние исследования их природа до конца не выяснена. Предполагается, что измерения линейной поля- ризации гамма-всплесков позволят отличить разные модели излучений этих явлений. Для измерения поляризации гамма-излучения используются сегментированные сцинтилляционные детекторы, которые позволяют не только из- мерить поляризацию, но и уменьшить эффекты мертвого времени, а также определить положение источника на небе. В работе проведено моделирование сегментированного детектора с помощью программного пакета Geant4. Исследо- вана эффективность локализации источника таким детектором. Приведена методика экспериментальной калибровки детектора для измерения мертвого времени.
Gamma-ray bursts are among the brightest transient electromagnetic phenomena in the Universe. Despite years of research, their nature has not been fully clarified. It is assumed that measurements of the linear polarization of gamma-ray bursts will make it possible to distinguish different radiation models of these phenomena. Segmented scintillation detectors are used to measure the polarization of gamma radiation, which make it possible to measure polarization, reduce the effects of dead time and determine the position of the source in the sky. We performed a simulation of the segmented detector using the Geant4 software package. The effectiveness of source localization using such a detector has been investigated. The method of experimental calibration of the detector for measuring dead time is presented.