bluuebunny/crossref_metadata_2025_split huggingface dataset:
Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation
Authors: Noh Jungsik , Isogai Tadamoto , Chi Joseph , Bhatt Kushal , Danuser Gaudenz 
Published: May 2021

AbstractMany cell regulatory systems implicate nonlinearity and redundancy among components. The regulatory network governing lamellipodial and lamellar actin structures is prototypical of such a system, containing tens of actin-nucleating and -modulating molecules with functional overlap and feedback loops. Due to instantaneous and long-term compensation, phenotyping the system response to perturbation provides limited information on the roles the targeted component plays in the unperturbed system. Accordingly, how individual actin regulators contribute to lamellipodial dynamics remains ambiguous. Here, we present a perturbation-free reconstruction of cause-effect relations among actin regulators by applying Granger-causal inference to constitutive image fluctuations that indicate regulator recruitment as a proxy for activity. Our analysis identifies distinct zones of actin regulator activation and of causal effects on filament assembly and delineates actin-dependent and actin-independent regulator roles in controlling edge motion. We propose that edge motion is driven by assembly of two independently operating actin filament systems. A record of this paper’s Transparent Peer Review process is included in the Supplemental Information.
Syn3 Gene Knockout Negatively Impacts Aspects of Reversal Learning Performance
Authors: Moore Alyssa , Linden Jérôme , Jentsch James D. 
Published: May 2021

AbstractBehavioral flexibility enables the ability to adaptively respond to changes in contingency requirements to maintain access to desired outcomes, and deficits in behavioral flexibility have been documented in many psychiatric disorders. Previous research has shown a correlation between behavioral flexibility measured in a reversal learning test and Syn3, the gene encoding synapsin III, which negatively regulates phasic dopamine release. Syn3 expression in the hippocampus, striatum, and neocortex is reported to be negatively correlated with reversal learning performance, so here, we utilized a global knockout line to investigate reversal learning in mice homozygous wildtype, heterozygous null, and homozygous null for the Syn3 gene. Compared to wildtype animals, we found a reversal specific effect of genetic Syn3 deficiency that resulted in a greater proportional increase in trials required to reach a preset performance criteria during contingency reversal, despite no observed genotype effects on the ability to acquire the initial discrimination. Behavioral flexibility scores, which quantified the likelihood of switching subsequent choice behavior following positive or negative feedback, became significantly more negative in reversal only for Syn3 homozygous null mice, suggesting a substantial increase in perseverative behavior in the reversal phase. Syn3 ablation reduced the number of anticipatory responses made per trial, often interpreted as a measure of waiting impulsivity. Overall, Syn3 expression negatively affected behavioral flexibility in a reversal specific manner but may have reduced waiting impulsivity.
Comparison of the neuraminidase antigenicity in recently circulating influenza A and vaccine viruses
Authors: Gao Jin , Li Xing , Wan Hongquan , Ye Zhiping , Daniels Robert 
Published: May 2021

ABSTRACTNeuraminidase (NA or N) antigens in circulating influenza viruses are not extensively evaluated for vaccine strain selection like hemagglutinin (HA or H) even though viral-based influenza vaccines include the recommended strain NA in varying amounts. As NA can also elicit a protective response, we assessed the antigenic similarity of the NAs from human H1N1 and H3N2 viruses that were prevalent between September 2019 to December 2020 to NAs from several recently recommended vaccine strains. To eliminate the dependence on isolates, the enzyme-linked lectin assay for analyzing NA antigenicity was performed with reverse genetic viruses carrying the same HA. Our results show that ferret antisera against NAs from the recommended H1N1 and H3N2 vaccine strains for the 2020-21 northern hemisphere influenza season recognize and inhibit the most prevalent circulating N1s and N2s, suggesting the NAs from the influenza A vaccine and circulating strains are antigenically similar. Comparisons of the recent N2s also revealed a bias in the reactivity of NA antisera from the egg and cell-based H3N2 vaccine strains due to a C-terminal substitution, indicating the C-terminus can influence N2 antigenicity and should receive consideration during the H3N2 strain selection.
Multiview super-resolution microscopy
Authors: Wu Yicong , Han Xiaofei , Su Yijun , Glidewell Melissa , Daniels Jonathan S. , Liu Jiamin , Sengupta Titas , Rey-Suarez Ivan , Fischer Robert , Patel Akshay , Combs Christian , Sun Junhui , Wu Xufeng , Christensen Ryan , Smith Corey , Bao Lingyu , Sun Yilun , Duncan Leighton H. , Chen Jiji , Pommier Yves , Shi Yun-Bo , Murphy Elizabeth , Roy Sougata , Upadhyaya Arpita , Colón-Ramos Daniel , La Riviere Patrick , Shroff Hari 
Published: May 2021

SummaryWe enhance the performance of confocal microscopy over imaging scales spanning tens of nanometers to millimeters in space and milliseconds to hours in time, improving volumetric resolution more than 10-fold while simultaneously reducing phototoxicity. We achieve these gains via an integrated, four-pronged approach: 1) developing compact line-scanners that enable sensitive, rapid, diffraction-limited imaging over large areas; 2) combining line-scanning with multiview imaging, developing reconstruction algorithms that improve resolution isotropy and recover signal otherwise lost to scattering; 3) adapting techniques from structured illumination microscopy, achieving super-resolution imaging in densely labeled, thick samples; 4) synergizing deep learning with these advances, further improving imaging speed, resolution and duration. We demonstrate these capabilities on more than twenty distinct fixed and live samples, including protein distributions in single cells; nuclei and developing neurons inCaenorhabditis elegansembryos, larvae, and adults; myoblasts inDrosophilawing imaginal disks; and mouse renal, esophageal, cardiac, and brain tissues.
PRDM proteins control Wnt/β-catenin activity to regulate craniofacial chondrocyte differentiation
Authors: Shull Lomeli C. , Kim Hyun Min , Lencer Ezra S. , Goyama Susumu , Kurokawa Mineo , Costello James C. , Jones Kenneth , Artinger Kristin B. 
Published: May 2021

SummaryCranial neural crest (NCC)-derived chondrocyte precursors undergo a dynamic differentiation and maturation process to establish a scaffold for subsequent bone formation, alterations in which contribute to congenital birth defects. Here, we demonstrate that transcription factor and histone methyltransferase proteins Prdm3 and Prdm16 control the differentiation switch of cranial NCCs to craniofacial cartilage. Loss of either results in hypoplastic and unorganized chondrocytes due to impaired cellular orientation and polarity. We show that PRDMs regulate cartilage differentiation by controlling the timing of Wnt/β-catenin activity in strikingly different ways: prdm3 represses while prdm16 activates global gene expression, though both by regulating Wnt enhanceosome activity and chromatin accessibility. Finally, we show that manipulating Wnt/β-catenin signaling pharmacologically or generating prdm3-/-;prdm16-/- double mutants rescues craniofacial cartilage defects. Our findings reveal upstream regulatory roles for Prdm3 and Prdm16 in cranial NCCs to control Wnt/β-catenin transcriptional activity during chondrocyte differentiation to ensure proper development of the craniofacial skeleton.HighlightsPrdm3 and Prdm16 are required for chondrocyte organization in vertebrate craniofacial cartilageLoss of Prdm3 and Prdm16 alters expression of Wnt/β-catenin signaling componentsPrdm3 and Prdm16 oppositely control global chromatin accessibilityPrdm3 and Prdm16 cartilage defects can be rescued pharmacologically or genetically
Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical
Authors: Alderley Carrie Louise , Edwards Greenrod Samuel Terrence , Friman Ville-Petri 
Published: May 2021

AbstractCrop losses to plant pathogens are a growing threat to global food security and more effective control strategies are urgently required. Biofumigation, an agricultural technique where Brassica plant tissues are mulched into soils to release antimicrobial plant allelochemicals called isothiocyanates (ITCs), has been proposed as an environmentally friendly alternative to agrochemicals. While biofumigation has been shown to suppress a range of plant pathogens, its effects on plant pathogenic bacteria remain largely unexplored. Here we used a laboratory model system to compare the efficacy of different types of ITCs against Ralstonia solanacearum plant bacterial pathogen. Additionally, we evaluated the potential for ITC-tolerance evolution under high, intermediate and low transfer frequency ITC exposure treatments. We found that allyl-ITC was the most efficient compound at suppressing R. solanacearum growth, and its efficacy was not improved when combined with other types of ITCs. Despite consistent pathogen growth suppression, ITC tolerance evolution was observed in the low transfer frequency exposure treatment, leading to cross-tolerance to ampicillin beta-lactam antibiotic. Mechanistically, tolerance was linked to insertion sequence movement at four positions in genes that were potentially associated with stress responses (H-NS histone like protein), cell growth and competitiveness (acyltransferase), iron storage ((2-Fe-2S)-binding protein) and calcium ion sequestration (calcium-binding protein). Interestingly, pathogen adaptation to the growth media also indirectly selected for increased ITC tolerance through potential adaptations linked with metabolism and antibiotic resistance (dehydrogenase-like protein) and transmembrane protein movement (Tat pathway signal protein). Together, our results suggest that R. solanacearum can rapidly evolve tolerance to allyl-ITC plant allelochemical which could constrain the long-term efficiency of biofumigation biocontrol and potentially shape pathogen evolution with plants.
Building information modelling (BIM) penetration in quantity surveying (QS) practice
Authors: Olatunji Oluwole Alfred , Lee Jane Jia Suen , Chong Heap-Yih , Akanmu Abiola Abosede 
Published: May 2021

PurposeThis study investigates building information modelling (BIM) penetration in quantity surveying (QS) practice by examining the significance attached to the benefits and barriers of BIM adoption, BIM capabilities and future directions of BIM in QS processes. A popular opinion amongst construction researchers is that BIM has the capacity to revolutionize the industry. The study draws-out information in the literature regarding discipline-specific penetration of BIM.Design/methodology/approachSnowball sampling method was used to obtain information through a survey questionnaire. A total of 73 participants, largely quantity surveyors in Western Australia, took part in the study. Reductionist methodology was used to identify key variables of QS-BIM competencies that are most significant statistically.FindingsBIM does not impose additional difficulties to traditional QS processes. Adherence to standard method of measurement and limited market demand do not hinder BIM deployment significantly. Quantity surveyors are able to use BIM to support their professional services once definitive design models are involved. In addition, the study identifies BIM penetration barriers to include constraints caused by centralised database management and interoperability issues, limitations imposed by market drivers, lack of in-house expertise to manage modelling needs and limited capability in software management.Practical implicationsFuture opportunities for skill development are in the areas noted in the findings. Whilst many studies have reported resistance and widespread scepticism amongst some construction disciplines regarding BIM adoption, this study finds BIM penetration in QS practice is considerable, a direction that could trigger further novel innovations.Originality/valueThe methodology reported in the study is novel. In addition, findings from the study inspires other discipline-specific studies to articulate their BIM-penetration trends so that t broad areas of construction can develop a balanced strategy around BIM and innovation development.
Towards dynamic processes-of-business processes: a new understanding
Authors: Cagnin Maria Istela , Nakagawa Elisa Yumi 
Published: May 2021

PurposeThe main purpose of this work is to overcome the difficulty to manage large, dynamic business processes formed from the complicated interplay of business processes of distinct organizations, which take part of strategic alliances of organizations (joint ventures and mergers and acquisitions) and have their software systems as part of complex, large software-intensive systems called Systems of Systems (SoS). Hence, the authors introduce the novel concept of Processes-of-Business Processes (PoP), deepening on their characteristics and related key terms.Design/methodology/approachBased on the systematically retrieved and analyzed literature and on the authors’ experience from research/industrial projects, the authors defined PoP and have applied them in the Brazilian public health SoS, which involve several organizations and their business processes and their independent software systems as well.FindingsProvided by PoP, a new understanding of the large, dynamic business processes of alliances of organizations is fundamental to advance both the state of the art and the state of the practice on business process management.Research limitations/implicationsThe adoption of PoP in other real-world scenarios is still required to get quantitative evidence.Practical implicationsThe adoption of PoP can certainly contribute to the success of new businesses and even promoting sustainability and longevity of SoS, which are in fact deeply impacted by business processes.Originality/valueThe concept of PoP can change the mindset of the research community and practitioners about how large, dynamic business processes should be seen and further managed.
Impact of Chinese firms’ political connections on EVA performance: the moderating roles of connection heterogeneity and product diversification
Authors: He Xiaoming , Chen Ao 
Published: May 2021

PurposeThis paper aims to explore the impact of a firm’s political connections on its economic value added (EVA) performance while treating connection heterogeneity and product diversification as moderators.Design/methodology/approachBased on data collected from 1,143 Chinese manufacturing listed firms in China’s A-share market from 2012 to 2017, this study conducted panel data analysis to investigate proposed relationships.FindingsThe findings provide evidence that political connections promote EVA performance of enterprises and both connection heterogeneity and product diversification negatively moderate the political connections – EVA performance relationship.Originality/valueDrawing sights from the resource-based view, this study investigates the influence of corporate political connections on EVA performance, considering contingent factors of connection heterogeneity and corporate strategy (i.e. product diversification). It, thus, contributes to the literature on political connections by providing additional evidence to explaining the inconclusive findings on the political connections–firm performance relationship and extending prior research by emphasizing the moderating roles of connection heterogeneity and corporate strategy. It also complements prior research on EVA performance by exploring its antecedents.
An analytical review of construction law research
Authors: Khademi Adel Tahereh , Modir Mohsen , Ravanshadnia Mehdi 
Published: May 2021

PurposeThis article investigates recent studies of construction law in different areas, including civil engineering; construction building technology; transportation; multidisciplinary studies and the environment, as well as their changing trends in the years between 2000 and 2019.Design/methodology/approachThe transformation trend of construction law is investigated based on collecting main keywords from the Web of Science (WoS) database selectively from different viewpoints and using Scientometric Analysis by CiteSpace and HistCite software. The top journals, top universities, and the most active countries in publishing and expanding construction law, keyword co-occurrence network, top keywords with the strongest citation bursts, cluster analysis, the most cited articles are determined both generally and yearly.FindingsBy interpreting the Scientometric results, focal points of legal issues and their changing trends during the last two decades are reviewed. Scholars’ data concerning interesting topics, construction law industry future needs, knowledge gaps, and speculation about future views and direction are obtained.Research limitations/implicationsRestrictions on data search, limiting the category of studies to a specific domain, and limiting research time to 20 years are some limitations of this article.Social implicationsAll these results address legal issues, comprehensive laws, plenary contracts, and efficient dispute resolution based on cultures and themes.Originality/valueGiven the importance of legal issues at all stages of the construction cycle, a review of this multidisciplinary and new science over the past two decades can provide hot issues, knowledge gaps, and a better view of the future.
Diamond in the rough? An “empty review” of research into “neurodiversity” and a road map for developing the inclusion agenda
Authors: Doyle Nancy , McDowall Almuth 
Published: May 2021

PurposeThe aims of the paper were to highlight the dearth of applied practitioner research concerning the expression of neurodiversity at work and develop an epistemological framework for a future research agenda.Design/methodology/approachA systematic empty review protocol was employed, with threea prioriresearch questions, inquiring as to the extent of neurodiversity research within mainstream work psychology, psychology in general and lastly within cross-disciplinary academic research. The results of the final search were quality checked and categorized to illustrate where studies relevant to practice are currently located.FindingsThe academic literature was found to be lacking in contextualized, practical advice for employers or employees. The location and foci of extracted studies highlighted a growing science-practitioner gap.Research limitations/implicationsThe research focused on common neurominority conditions such as autism and dyslexia; it is acknowledged that the neurodiversity definition itself is broader and more anthropological in nature. A need for a comprehensive research agenda is articulated, and research questions and frameworks are proposed.Practical implicationsGuidance is given on applying disability accommodation to both individual and organizational targets.Social implicationsThe disability employment gap is unchanged since legislation was introduced. The neurodiversity concept is no longer new, and it is time for multi-disciplinary collaborations across science and practice to address the questions raised in this paper.Originality/valueThis paper offers an original analysis of the neurodiversity paradox, combining systematic inquiry with a narrative synthesis of the extant literature. The conceptual clarification offers clear directions for researchers and practitioners.
Physical health symptoms among Ethiopian returnees who were trafficked aboard
Authors: Gezie Lemma Derseh , Atinafu Asmamaw 
Published: May 2021

Purpose
There is a growing evidence of considering human trafficking as a severe form of violence which usually results in various health outcomes including symptoms of physical health problems. This study aims to examine the magnitude of physical health symptoms during the trafficking period and compare it with that of the period prior to it.

Design/methodology/approach
A total of 1,387 trafficking returnees from abroad via three trafficking corridors of Ethiopia were recruited consecutively. Among socio-demographic and other characteristics, data on various physical health symptoms that were experienced during the trafficking period and in the two years prior to trafficking were collected. The proportions of physical health symptoms experienced during the two periods were determined and compared using chi-square test.

Findings
Among all participants, 598 (46.79%) of them experienced weight loss during the trafficking period and 106 (8.28%) before the trafficking period. The extra 38.50% which was experienced during the trafficking period was statistically significant (p < 0.0001). Similar significant differences were observed for symptoms such as forgetfulness (p < 0.0001), stomachache (p = 0.0039), gynecological problems (p = 0.041), bone fracture (p < 0.0001), back pain (p < 0.0001), and wound (p < 0.0001); but not significantly different for symptoms such as skin disease (p-value = 0.1944), tooth pain (p-value =0.6587) and sight problem (p-value = 0.1306).

Originality/value
Comparing the frequencies of physical health symptoms before and during the trafficking period among victims of trafficking is relatively a new approach to measure the nexus between Human trafficking and health problems.
Effect of material thickness and reduction ratio on roughness transfer in skin-pass rolling to DC04 grade sheet materials
Authors: Özakın Batuhan , Çolak Bilal , Kurgan Naci 
Published: May 2021

Purpose
The last stage of the cold rolling process is skin-pass rolling and one of its most significant goals is to obtain appropriate topography on the surface of the sheet steel used extensively such as in automotive industry. The purpose of this paper is to investigate the effect of thickness change and various reduction ratios on roughness transfer of DC04 grade sheet material.

Design/methodology/approach
DC04 grade sheet materials with different reduction ratios and several thicknesses were subjected to skin-pass rolling process in the rolling equipment with a two-high roll. Some roughness parameters were determined as a result of roughness measurements from the surfaces of roughened sheet materials.

Findings
While the roughness transfer is higher in 1-mm thick material in reduction ratios up to 430 micrometers; in reduction ratios above 430 micrometers, it is higher for 1.5-mm thick materials. As the reduction ratio increases in DC04 grade sheet materials, the homogeneity of the roughness distribution in 1-mm thickness sheet material deteriorates, while the roughness distribution in 1.5-mm thickness sheet material is more homogeneous.

Originality/value
This paper demonstrates how material thickness and reduction ratio affect the roughness transfer in skin-pass rolling. The results obtained can be used by optimizing in manufacturing processes.