bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sirt1 Protects Against Hippocampal Atrophy and its Induced Cognitive Impairment in Middle-aged Mice
Authors: Sun Zuhao , Zhao Shuang , Suo Xinjun , Dou Yan 
Published: December 2021

Abstract
Background: Sirtuin 1 (Sirt1) is a recognized longevity gene and has been shown to be associated with aging and its related diseases. Hippocampal volume is considered to be the most sensitive brain imaging phenotype for cognition, but the effect of Sirt1 on hippocampal morphology during aging has not been reported. Results: Herein, we investigated the effect of conditional Sirt1 knockdown on hippocampal volume in middle-aged mice, as well as its cognitive function and the underlying molecular mechanisms. Brain structural magnetic resonance imaging (MRI) showed that adeno-associated virus (AAV) mediated hippocampal Sirt1 knockdown caused hippocampal atrophy in 8-month-old mice. Open field test (OFT) and Morris Water Maze (MWM) test revealed that hippocampal Sirt1 knockdown significantly weakened spatial learning and memory of mice without effect on anxiety and exploratory behavior. Western blotting analysis showed that p-tau levels were significantly increased while PSD95 levels were obviously reduced, indicating that hippocampal Sirt1 knockdown could activate tau pathology and synaptic damage.Conclusions: This work revealed that Sirt1 is an important protective gene against hippocampal atrophy and its induced cognitive impairment during aging, providing potential therapeutic targets for the prevention and intervention of aging-related neuropsychic diseases.
Disconnections in Personal Neglect
Authors: Bertagnoli Sara , Pacella Valentina , Rossato Elena , Jenkinson Paul M , Fotopoulou Akaterini , Scandola Michele , Moro Valentina 
Published: December 2021

Abstract
Personal neglect is a disorder in the perception and representation of the body that causes the patients to behave as if the contralesional side of their body does not exist. This clinical condition has not been adequately investigated in the past as it has been considered a symptom of unilateral spatial neglect, which has mainly been studied with reference to extrapersonal space. Only a few studies with small samples have investigated the neuroanatomical correlates of personal neglect, and these have mainly focused on discrete cortical lesions and modular accounts, as well as being based on the hypothesis that this disorder is associated with somatosensory and spatial deficits. In the present study, we tested the novel hypothesis that personal neglect may be associated not only with discrete cortical and subcortical lesions, but also with disconnections of white matter tracts. We performed an advanced lesion analyses in a large sample of 104 right hemisphere damaged patients, 68 of whom were suffering from personal neglect. Results from the analyses of the grey and white matter were controlled for co-occurrent clinical variables such as extrapersonal neglect, anosognosia for hemiplegia and motor deficits, along with other lesion-related variables such as lesion size, the interval from the lesion onset to neuroimaging recordings. Our results reveal that personal neglect is associated with lesions in a medial network which involves the temporal cortex (Heschl’s gyrus), the ventro-lateral nuclei of the thalamus, and the fornix. This suggests that personal neglect involves a convergence between sensorimotor processes, spatial representation and the processing of self-referred information (episodic memory).
FLT3 Inhibitors as Maintenance Therapy Post Hematopoietic Cell Transplantation for Acute Myeloid Leukemia (AML): A Systematic Review and Meta-Analysis
Authors: Zheng Xinhui , Li Xiangjun , Jiang Er-Lie , Lv Liwei 
Published: December 2021

Abstract
The purpose of this paper is to systematically analyze the outcome of FLT3 inhibitors maintenance treatment following hematopoietic stem cell transplantation (HSCT) for patients suffering from FLT3-ITD-mutated acute myeloid leukemia (AML). Pubmed, Embase, and Cochrane Library databases were retrieved before November 2021. Fifteen studies were included eventually containing six without control and nine with control. Thirteen studies evaluated sorafenib, and two assessed quizartinib and midostaurin, separately. Via survival analysis, the main outcomes in the FLT3 inhibitors group were improved greatly with the hazard ratio(HR) of overall survival of 0.38 (95% confidence interval [CI], 0.29-0.49; P < 0.001), HR of leukemia-free survival of 0.35 (95%CI, 0.27-0.47; P < 0.001) and HR of cumulative incidence of relapse of 0.32 (95%CI, 0.23-0.45; P < 0.001). Moreover, the TKI use didn’t seem to increase the incidence of graft-versus-host disease (GVHD) and adverse effects in statistics. Through subgroup analysis, MRD-positive patients before and after HCT, and MRD-negative patients before HCT might benefit a lot from sorafenib maintenance.
Impact of Intradialytic Fiber Clotting on Dialyzer Extraction and Solute Removal: A Randomized Cross-Over Study
Authors: Vanommeslaeghe Floris , Josipovic Iván , Boone Matthieu , Biesen Wim Van , Eloot Sunny 
Published: December 2021

Abstract
Previous studies revealed the importance of biocompatibility, anticoagulation strategy, and dialysis mode and duration on fiber blocking at the end of a hemodialysis session. The present study was set up in ten hemodialysis patients to relate fiber patency to dialyzer extraction and removal of small and middle molecules. With only 1/4th of the regular anticoagulation dose, and using a Solacea™ 19H and FX800 CorDiax dialyzer, fiber patency was quantified using 3D micro-CT scanning for different dialysis durations (i.e. 60, 120 and 240min). While Solacea™ showed good performance in all test sessions, fiber blocking in the FX800 CorDiax did not follow a linear process during dialysis, but was rather accelerated near the end of dialysis. Dialyzer extraction ratios were correlated with the percentages of open fibers. While the fiber blocking process affected extraction ratios (i.e. for phosphorus and myoglobin in the FX800 CorDiax), it had only minor impact on the removal of toxins up to at least 12kDa.
Cultural Adaptation and Validation of the Chinese Version of the Systemic Lupus Erythematosus Activity Questionnaire in a Cohort of Chinese Patients with Lupus
Authors: Pan Lei , Zhang Chenyu , Huang Yan , Xi Huiqin 
Published: December 2021

Abstract
Objectives:To examine the responsiveness, reliability, and validity of Chinese version of Systemic Lupus Erythematosus Questionnaire (SLAQ). Methods:A cross-sectional observational study was designed for this research. The principal attributes evaluated were linguistic and cultural adaption, reliability, and validity.Standardized response means and acceptability were calculated to estimate the responsiveness of the SLAQ. Cronbach’s alpha was used to evaluate its reliability. The Spearman correlation coefficient was used to assess the criterion validity by calculating the association with measurement of other lupus disease activity.ResultsThe response rate for the pilot study was 99.9%. The mean time to complete the questionnaire was 6.7±2.8 min. The Chinese version of SLAQ had good reliability, with a Cronbach’s alpha of 0.857. Cronbach’s alpha for the test-retest reliability was 0.878. For validity, the SLAQ was correlated with SLAM, SLEDAI-2000 and SLEDAI-no lab. The correlation was good at 0.832, 0.506 and 0.668 respectively.Conclusion:The Chinese version of the SLAQ questionnaire has adequate responsiveness, reliability, and validity in our cohort, and it is a helpful and meaningful tool for assessing disease activity in lupus patients without laboratory results.
Gonadal Hormone Trigger Traf6-mediated Dynamic Microglial Alterations That Correlate With Depressive Symptomatology
Authors: Peng Rui , Dai Wen , Li Di , Li Yan 
Published: December 2021

Abstract
Backgrounds: Gonadal hormone deficiency is associated with the development of depression, but what mediates this association is unclear. To test the possibility that it reflects neuroimmune and neuroinflammatory processes, we analyzed how gonadal hormone deficiency and replacement affect microglial activation and inflammatory response during the development of depressive symptomatology in gonadectomized male mice. Methods: Adult male ICR mice received gonadectomy. Gonadal hormone levels, neuroinflammation, mciroglial activation and depressive behaviors were evaluated 7 days, 14 days, and 30 days later. Furthermore, the neuroprotective mechanism of treatment with testosterone and estradiol on depressive symptomatology were also observed.Results: Testosterone level and the ratio of testosterone to estradiol in the serum and brain tissue of mice exposed to 3-35 days of chronic unpredictable stress were much lower than in control animals. Gonadal hormone sustained deficiency in gonadectomized mice and subsequent led to acute inflammation at day 7 following castration. Activating microglia in mice exposed to 7 days of castration subsequently suppressed the proliferation of microglia, such that their numbers in hippocampus and cortex were lower than the numbers in sham-operated mice after 30 days of castration. Here, we showed that gonadal hormone deficiency induces Traf6-mediated microglia activation, a type of inflammatory mediator. Microglia treated in this way for long time showed down-regulation of activation markers, abnormal morphology and depressive-like behaviors. Restoration and maintenance of a fixed ratio of testosterone to estradiol significantly suppressed microglial activation, neuronal necroptosis, dramatically inducing hippocampal neurogenesis and reducing depressive behaviors via the suppression of Traf6/TAK1 pathway. Conclusions: These findings suggest that activated or immunoreactive microglia contribute to gonadal hormone deficiency-induced depression, as well as testosterone and estradiol exert synergistic anti-depressant effects via suppressing microglial activaton in gonadectomized male mice, possibly through Traf6 signaling.
Horizontal Grid Spacing Comparison Among Random Forest Algorithms to Nowcast Cloud-to-Ground Lightning Occurrence
Authors: FATA ALICE LA , Amato Federico , Bernardi Marina , D'Andrea Mirko , Procopio Renato , Fiori Elisabetta 
Published: December 2021

Abstract
This paper discusses the use of Random Forest (RF), a popular Machine Learning (ML) algorithm, to perform spatially explicit nowcasting of cloud-to-ground lightning occurrence. An application to the Italian territory and the surrounding seas is presented. Specifically, 1-hour ahead lightning occurrences over the months of August, September and October from 2017 to 2019 have been modelled using a dataset including geo-environmental features. Results obtained with three different spatial resolutions have been compared, for nowcasting both positive and negative strokes. The features’ importance resulting from the best RF models showed how datadriven models are able to identify the relationships between meteorological variables, in agreement with previous physically based knowledge of the phenomenon. The encouraging results obtained in terms of forecasting accuracy support the idea to use ML-based algorithms in early warning procedures for disaster risk management.
Cutaneous Nerve Fibers Participate in the Progression of Psoriasis by Linking Epidermal Keratinocytes and Immunocytes
Authors: Chen Si-Qi , Chen Xue-Yan , Cui Ying-Zhe , Yan Bing-Xi , Zhou Yuan , Wang Zhao-Yuan , Xu Fan , Huang Yan-Zhou , Zheng Yu-Xin , Man Xiao-Yong 
Published: December 2021

Abstract
Recent studies have illustrated that psoriatic lesions are innervated by dense sensory nerve fibers. Psoriatic plaques appeared to improve after central or peripheral nerve injury. Therefore, the nervous system may play a vital role in psoriasis. We aimed to clarify the expression of nerve fibers in psoriasis and their relationship with immune cells and keratinocytes, and to explore the effect of skin nerve impairment. Our results illustrated that nerve fibers in psoriatic lesions increased and were closely innervated around immune cells and keratinocytes. RNA-seq analysis showed that peripheral sensory nerve-related genes were disrupted in psoriasis. In spinal cord hemi-section mice, sensory impairment improved psoriasiform dermatitis and inhibited the abnormal proliferation of keratinocytes. Botulinum toxin A alleviated psoriasiform dermatitis by inhibiting the secretion of calcitonin gene-related peptide. Collectively, cutaneous nerve fibers participate in the progression of psoriasis by linking epidermal keratinocytes and immunocytes. Neurological intervention may be a new treatment strategy for psoriasis.
Global Core Indicators for Measuring WHO’s Paediatric Quality- of-care Standards in Health Facilities: Development and Expert Consensus
Authors: Muzigaba Moise , Chitashvili Tamar , Choudhury Allysha , Were Wilson M , Diaz Theresa , Strong Kathleen L , Jackson Debra , Requejo Jennifer , Detjen Anne , Sacks Emma 
Published: December 2021

Abstract
BackgroundThere are currently no global recommendations on a parsimonious and robust set of indicators that can be measured routinely or periodically to monitor quality of hospital care for children and young adolescents. We describe a systematic methodology used to prioritize and define a core set of such indicators and their metadata for progress tracking, accountability, learning and improvement, at facility, (sub) national, national, and global levels.MethodsWe used a deductive methodology which involved the use of the World Health Organization Standards for improving the quality-of-care for children and young adolescents in health facilities as the organizing framework for indicator development. The entire process involved 9 complementary steps which included: a rapid literature review of available evidence, the application of a peer-reviewed systematic algorithm for indicator systematization and prioritization, and multiple iterative expert consultations to establish consensus on the proposed indicators and their metadata. ResultsWe derived a robust set of 25 core indicators and their metadata, representing all 8 World Health Organization quality standards, 40 quality statements and 520 quality measures. Most of these indicators are process-related (64%) and 20% are outcome/impact indicators. A large proportion (84%) of indicators were proposed for measurement at both outpatient and inpatient levels. By virtue of being a parsimonious set and given the stringent criteria for prioritizing indicators with “quality measurement” attributes, the recommended set is not evenly distributed across the 8 quality standards. ConclusionsTo support ongoing global and national initiatives around paediatric quality-of-care programming at country level, the recommended indicators can be adopted using a tiered approach that considers indicator measurability in the short-, medium-, and long-terms, within the context of the country’s health information system readiness and maturity. However, there is a need for further research to assess the feasibility of implementing these indicators across contexts, and the need for their validation for global common reporting.
Recommend-Response and Complications Comparing Radiofrequency Ablation Versus Parathyroidectomy for Secondary Hyperparathyroidism in Dialysis Patients
Authors: Ren Mian , Zheng Danna , Wu Juan , Liu Yueming , Peng Chengzhong , Shen Wei , Lin Bo 
Published: December 2021

Abstract
Introduction

: To compare the efficacy and safety of ultrasound(US)-guided radiofrequency ablation (RFA) and parathyroidectomy (PTX) in the treatment of secondary hyperparathyroidism (SHPT).
Methods

In this retrospective study, we divided patients into PTX (n=53) and RFA (n=47) groups. The primary outcomes were the proportion of patients achieved the target range of iPTH concentrations(124-558pg/mL) and the long-term prognosis. The secondary outcomes were the differences in the changes of iPTH, calcium, and phosphorus over time and the incidence of adverse events.
Results

There was rarely difference in baseline characteristics between the two groups. Primary outcomes: The iPTH concentrations of 25.8% of patients in the PTX group and 51.3% of the RFA group were within the recommended range at the endpoint (P=0.031). Survival analysis revealed that the difference in all-cause mortality and cumulative response rate between the two groups was not statistically significant (P=0.902, P=0.141, respectively). Secondary outcomes: The iPTH concentrations in PTX group and RFA group dropped sharply after treatment and were 82.30±163.21pg/mL and 279.96±306.57pg/mL (P<0.001). There was no difference in the trend of iPTH, calcium, and phosphorus levels between the two groups over time(P>0.05). In addition, the incidence of infection and the hospital stay in the RFA group were significantly less. And preoperative bone-specific alkaline phosphatase concentration was a risk factor for postoperative hypocalcemia.
Conclusion

US-guided RFA was minimally invasive and no less than PTX in terms of cumulative response rate and complications in the treatment of severe SHPT in maintenance dialysis patients and may be used as an alternative technique to PTX, which need further studies to confirm.
On Outage Analysis in Overlay CCRN With RF Energy Harvesting and Co-Channel Interference
Authors: GHOSH SUTANU , Maity Santi P. , Acharya Tamaghna 
Published: December 2021

Abstract
This paper explores the impact of co-channel interference (CCI) on the link outage and radio frequency (RF) energy harvesting (EH). For analysis, co-operative cognitive radio network (CCRN) architecture is considered as system model that supports one-way primary user (PU) and two-way secondary user (SU) communications, using an overlay mode of spectrum sharing. Closed form outage expressions are derived for both PU and SU network in presence of multiple antennas at PUs and CCI at SUs. The effect of CCI on the system performance is studied with respect to interference-to-noise-ratio (INR), transmission power, number of antennas and number of CCI sources. Performance gains are found to achieve ~ 20% and ~ 15% for PU and SU outage in two antenna system over a single antenna one.
Longitudinal Multi-omic Phenotyping Reveals Host-microbe Responses to Bariatric Surgery, Glycaemic Control and Obesity
Authors: Penney Nicholas , Yeung Derek , Garcia-Perez Isabel , POSMA Joram , Kopytek Aleksandra , Garratt Bethany , Ashrafian Hutan , Frost Gary , Marchesi Julian , Purkayastha Sanjay , Hoyles Lesley , Darzi Ara , Holmes Elaine 
Published: December 2021

Abstract
Resolution of type-2 diabetes (T2D) is common following bariatric surgery, particularly Roux-en-Y gastric bypass (RYGB). However, the underlying mechanisms have not been fully elucidated. To address this we compared the integrated serum, urine and faecal metabolic profiles of obese participants with and without T2D (n=81, T2D=42) with participants who underwent RYGB or sleeve gastrectomy (pre and 3-months post-surgery; n=27), taking diet into account. We co-modelled these data with shotgun metagenomic profiles of the gut microbiota to provide a comprehensive atlas of host-gut microbe responses to bariatric surgery, weight-loss and glycaemic control at the systems level. Bariatric surgery reversed a number of disrupted pathways characteristic of T2D. The differential metabolite set representative of bariatric surgery overlapped with both diabetes (19.3% commonality) and BMI (18.6% commonality). However, the percentage overlap between diabetes and BMI was minimal (4.0% commonality), consistent with weight-independent mechanisms of T2D resolution. The gut microbiota was more strongly correlated to BMI than T2D, although we identified some pathways such as amino acid metabolism that correlated with changes to the gut microbiota and which influence glycaemic control. Improved understanding of GM-host co-metabolism may lead to novel therapies for weight-loss or diabetes.
Reverse Transcription Recombinase-aided Amplification Assay for H5 Subtype avian Influenza Virus
Authors: Wang Suchun , Li Yang , zhang fuyou , Jiang Nan , Zhuang Qingye , Hou Guangyu , Jiang Lijian , Yu Jianmin , Yu Xiaohui , Liu Hualei , Zhao Chenglong , Yuan Liping , Huang Baoxu , Wang Kaicheng 
Published: December 2021

Abstract
Background: The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. Methods: In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39°C), and the results were obtained within 20 min. Results: The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 103 RNA copies/μL at a 95% confidence interval according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the RT-RAA assay in 420 avian clinical samples was 0.983 (p < 0.001). The sensitivity for avian clinical sample detection was 97.26% (95% CI, 89.56–99.52%), and the specificity was 100% (95% CI, 98.64–100%). Conclusions: These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
Quercetin and Luteolin Are Single-digit Micromolar Inhibitors of the SARS-CoV-2 RNA-dependent RNA Polymerase
Authors: Munafò Federico , Donati Elisa , Brindani Nicoletta , Ottonello Giuliano , Armirotti Andrea , De Vivo Marco 
Published: December 2021

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly become a global health pandemic. Among the viral proteins, RNA-dependent RNA polymerase (RdRp) is responsible for viral genome replication and has emerged as one of the most promising targets for pharmacological intervention against SARS-CoV-2. To this end, we experimentally tested luteolin and quercetin for their ability to inhibit the RdRp enzyme. These two compounds are ancestors of flavonoid natural compounds known for a variety of basal pharmacological activities. Luteolin and quercetin returned a single-digit IC50 of 4.6 µM and 6.9 µM, respectively. Then, through dynamic docking simulations, we identified possible binding modes of these compounds to a recently published cryo-EM structure of RdRp. Collectively, these data indicate that these two compounds are a valid starting point for further optimization and development of a new class of RdRp inhibitors to treat SARS-CoV-2 and potentially other viral infections.