bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactylation fuels nucleotide biosynthesis and facilitates deuterium metabolic imaging of tumor proliferation in H3K27M-mutant gliomas
Authors: Batsios Georgios , Taglang Céline , Udutha Suresh , Gillespie Anne Marie , Robinson Simon P , Phoenix Timothy , Mueller Sabine , Venneti Sriram , Koschmann Carl , Viswanath Pavithra 
Published: January 2025

ABSTRACTOncogenes hyperactive lactate production, but the mechanisms by which lactate facilitates tumor growth are unclear. Here, we demonstrate that lactate is essential for nucleotide biosynthesis in pediatric diffuse midline gliomas (DMGs). The oncogenic histone H3K27M mutation upregulates phosphoglycerate kinase 1 (PGK1) and drives lactate production from [U-13C]-glucose in DMGs. Lactate activates the nucleoside diphosphate kinase NME1 via lactylation and promotes the synthesis of nucleoside triphosphates essential for tumor proliferation. Importantly, we show that this mechanistic link between glycolysis and nucleotide biosynthesis provides a unique opportunity for deuterium metabolic imaging of DMGs. Spatially mapping2H-lactate production from [6,6-2H]-glucose allows visualization of the metabolically active tumor lesion and provides an early readout of response to standard-of-care radiation and targeted therapy that precedes extended survival and reflects pharmacodynamic alterations at the tissue level in preclinical DMG modelsin vivoat clinical field strength (3T). In essence, we have identified an H3K27M-lactate-NME1 axis that promotes DMG proliferation and facilitates non-invasive metabolic imaging of DMGs.STATEMENT OF SIGNIFICANCEThis study establishes a role for lactate in driving nucleotide biosynthesis in DMGs. Importantly, imaging lactate production from glucose using DMI provides a readout of tumor proliferation and early response to therapy in clinically relevant DMG models. Our studies lay the foundation for precision metabolic imaging of DMG patients.
1,3- and 1,4-linked Polysaccharides Uptake in Intestinal Cells Relies on Clathrin/Dynamin 1/Rab5-dependent Endocytosis
Authors: Liao Wenfeng , Cao Dianxiu , Wang Ying , Du Zhenyun , Yao Jian , Dou Pengfei , Zheng Yuandong , Wang Zhiming , Chen Xia , Wang Peipei , Chen Hao , Diao Xingxing , Wang Kaiping , Ding Kan 
Published: January 2025

AbstractNatural polysaccharides are considered to be a promising feedstock for manufacturing sustainable materials. However, polysaccharides are believed by most biologists that they could not be absorbed by intestinal mucosa to enter circulation system owing to their high hydrophilicity and large molecule size. Herein, β-1,3-linked glucan (GFPBW1) and α-1,4-linked glucan (WGE) is used as the model polysaccharide and the transport experiments show that different linked and charged polysaccharides can permeate Caco-2 cell monolayers. Thein vivoexperiments demonstrate that GFPBW1 and WGE are detectable in rodent plasma and liver after oral administration the isotope (99mTc,3H) and fluorescein labeled polysaccharides. Further study show that polysaccharide uptake requires clathrin heavy chain (CLTC) and its associated factors Rab5 and dynamin1 in intestinal cells using gene-knockdown strategies and inhibitors. Strikingly, polysaccharide absorption is attenuated in bothCLTCintestine deficient mice and Rab5, dynamin1 conventional-knockout mice. Importantly, membrane receptors bone morphogenetic protein receptor type IA (BMPRIA), Dectin-1 and epidermal growth factor receptor (EGFR) are also critical for specific structural polysaccharide internalization. These findings provide novel insight to understand polysaccharide absorption mechanism and lay foundation for oral polysaccharide- based new drugs development.
Increased sensorimotor noise in Tourette syndrome
Authors: Gialopsou Aikaterini , Houlgreave Mairi , Jackson Stephen R. 
Published: January 2025

SummaryTourette syndrome (TS) is a neurological disorder characterised by the occurrence of vocal and motor tics1. The pathophysiology of TS has been linked to a substantial reduction in the number of inhibitory GABAergic interneurons found within the striatum2, leading to increased neural ‘noise’ within the cortical-striatal-thalamic-cortical [CSTC] circuit implicated in movement production3. In the current study, we used electroencephalography (EEG) to investigate increased neural noise in a group of 19 adults with TS compared to a matched neurotypical control group. We operationalised neural noise in this study as increased trial-by-trial variability in the magnitude and/or the timing of responses to a discrete somatosensory stimulation event. Specifically, we examined trial-by-trial variability in responses to a single pulse of median nerve electrical stimulation [MNS]. Our results demonstrate that the P100 somatosensory evoked potential (SEP), which has been associated with conscious perception of tactile stimuli11, was significantly increased in the TS group. Importantly, however, while the timing, temporal variability, and spatial topography of early- and mid-latency SEP components (e.g., N20, P45, N60, P100) did not differ in the TS group, when compared to matched controls, trial-by-trial variability was substantially increased in the TS group, but this was normalised in response to stimulation. These findings may indicate that the trial-by-trial recruitment of neuronal sensorimotor populations is less stable at rest in individuals with TS compared to controls but may normalise in response to stimulation.
Selective attention network in naturalistic auditory scenes is object and scene specific
Authors: Wikman Patrik , Muukkonen Ilkka , Kauramäki Jaakko , Laaksonen Ville , Varis Onnipekka , Petkov Christopher , Rauschecker Josef 
Published: January 2025

AbstractEveryday auditory scenes often contain overlapping sound objects, requiring selective attention to isolate relevant objects from irrelevant background objects. This study examined how selective attention shapes neural representations of naturalistic sound scenes in the auditory cortex (AC). Using functional magnetic resonance imaging, we recorded brain activity from participants (n = 20) as they attended to a designated object in scenes comprising three overlapping sounds. Scenes were constructed in two manners: one where each object belonged to a different category (speech, animal, instrument) and another where all objects were from the same category. Attending to speech consistently enhanced activations in lateral AC subfields, while attention to animal and instrument sounds preferentially modulated medial subfields, supporting models where attention modulates feature-selective neural gain in AC. Remarkably, however, spatial pattern analysis revealed that the attended object dominated the AC activation patterns of the entire scene in a manner depending on both object type and scene composition: When the objects of the scene belonged to different categories, attended objects dominated fields processing higher-level category-specific features. In contrast, when all scene objects shared the same category, dominance shifted to fields processing low-level acoustic features. Thus, attention seems to dynamically prioritize the features offering maximal contrast within a given context, emphasizing object-specific patterns in feature-similar scenes and category-level patterns in feature-diverse scenes. Our results support models where top-down signals not only modulate gain but also affect several steps of auditory scene decomposition and analysis – influencing stream segregation and gating of higher-level processing in a contextual manner, adapting to specific auditory environments.
GABABreceptor-mediated potentiation of ventral medial habenula glutamatergic transmission in GABAergic and glutamatergic interpeduncular nucleus neurons
Authors: Stinson Hannah E. , Ninan Ipe 
Published: January 2025

AbstractThe medial habenula (MHb)-interpeduncular nucleus (IPN) pathway plays an important role in information transferring between the forebrain and the midbrain. The MHb-IPN pathway has been implicated in the regulation of fear behavior and nicotine addiction. The synapses between the ventral MHb and the IPN show a unique property, i.e., an enhancement of synaptic transmission upon activation of GABABreceptors. This GABABreceptor-mediated potentiation of ventral MHb-IPN synaptic transmission has been implicated in regulating fear memory. Although IPN is known to contain parvalbumin (PV) and somatostatin (SST) GABAergic neurons and vesicular glutamate transporter 3 (VGLUT3)-expressing neurons, it is unknown how GABABreceptor activation affects ventral MHb-mediated glutamatergic transmission onto these three subtypes of IPN neurons. Our studies show robust glutamatergic connectivity from ventral MHb to PV and SST neurons in the IPN, while the ventral MHb-mediated glutamatergic transmission in IPN VGLUT3 neurons is weak. Although activation of GABABreceptors produces a robust potentiation of ventral MHb-mediated glutamatergic transmission in PV neurons, we observed a modest effect in IPN SST neurons. Despite the diminished basal synaptic transmission between ventral MHb and IPN VGLUT3 neurons, activation of GABABreceptors causes transient conversion of non-responding ventral MHb synapses into active synapses in some IPN VGLUT3 neurons. Thus, our results show strong ventral MHb connectivity to GABAergic IPN neurons compared to VGLUT3-expressing IPN neurons. Furthermore, GABABreceptor activation produces a differential effect on ventral MHb-mediated glutamatergic transmission onto PV, SST, and VGLUT3 neurons in the IPN.
Innovations in skin aging: biomimetic exosomesversusnatural exosomes
Authors: Salvaggio Antonio , Privitera Anna , Iannello Giulia , Brundo Maria Violetta 
Published: January 2025

AbstractIn this study, we compared the effectiveness of two commercial products contain biomimetic and natural exosomes on skin aging used two cells line, keratinocytes and fibroblasts. MTT assay and scratch-wound assay were employed to evaluate theirin vitroeffects. The treatment with product containing natural exosomes purified from bovine colostrum and loaded with growth factors and cytokines purified from bovine colostrum (AMPLEX PLUS technology), was able to significantly enhance cell proliferation of fibroblasts and keratinocytes at 24 hours compared to cells treatment with product containing biomimetic exosomes and compared to untreated cells (control). Product containing natural exosomes was also able to close the wound scratch after 24 hours. The obtained results suggest that the product containing exosomes derived from bovine colostrum has excellent structural and functional stability offer great potential as natural product for skin aging damage and wound healing.
Modeling of GyrA, MexB, FtsI, AtpD Protein Variants In Multidrug ResistantAcinetobacter baumannii
Authors: Nik Mohd Nazri Nik Zakuan Hakim , Jusoh Siti Azma , Singh Kirnpal Kaur Banga , Ismail Mohamad Izwan 
Published: January 2025

ABSTRACTAcinetobacter baumanniiis a Gram-negative nosocomial pathogen known to manifest numerous drug resistances against major antibiotic classes. Compounded by its pathogenicity and virulence, it is considered globally as a top priority threat among the ESKAPE pathogens. The GyrA, MexB, FtsI and AtpD proteins inA. baumanniistrain PR07 have been proven to mutate under exposure to ciprofloxacin, meropenem, imipenem and erythromycin, respectively. While the genomic data is useful, the impact of the mutations on the protein structure and function is not well understood. To obtain a deeper understanding, the protein structures were analyzed using structural bioinformatics tools. Here, the PR07 GyrA, MexB, FtsI and AtpD protein sequence data from NCBI were compared between ESKAPE pathogens and otherA. baumanniispecies. MexB and AtpD structures were retrieved from the PDB database, while AlphaFold was used to construct protein structure predictions for GyrA and FtsI. MSA analyses identified mutations GyrA S81L, MexB S181L, FtsI P508, FtsI A515V, FtsI A579T and AtpD A166V mutations to be unique among the selected bacteria species. The mutation sites for all four target proteins were found to be within proximity to the potential binding sites. GyrA S81L, MexB S181L, FtsI A515V and FtsI A579T rigid protein models have shown loss of inter-residue polar hydrogen bonds, while AtpD A166V caused no observable changes. The mutations reported in PR07 therefore may potentially be significant contributors to its acquired resistance towards the target antibiotics.
Buildability-led procurement: a new model for future construction projects delivery
Authors: Osuizugbo Innocent Chigozie 
Published: January 2025

Purpose
The construction projects tends to perform poorly. Non-integration of buildability into the project procurement process is one of the reasons for the observed poor performance. To address this issue and close the gap in the existing knowledge, this paper proposes buildability-led procurement (BLP), a new procurement method for future construction projects in view to enhance buildability of complex private and public projects delivery as well as improving both organisation and project performance.

Design/methodology/approach
This paper adopts qualitative research approach and uses an integrated literature review method to synthesise literature from diverse domain so as to propose a five-stage BLP plan for implementation in construction industry.

Findings
Based on the information gleaned from the literature review, a BLP model was conceptualised. Adoption of the BLP model would lead to significant improvement in project outcomes. The model’s main innovation lies in the possibility of providing an optimised design solution for the project at the design stage.

Originality/value
This study proposed an innovative procurement method with practical plan for implementation and the possibility for improved project performance in the construction industry. BLP model implementation may lead to reduced waste, rework and removal of obstacles experienced during the construction phase. The proposed model marks a new strategy in procurement which enables the delivery of best quality project within a reasonable and justifiable cost and time in complex private and public projects. Hence, it makes a valuable contribution towards the buildability enhancement of complex projects as well as organisation and project performance improvement.
Delays to distributed ledgers: examining the rationale for leveraging blockchain technology in construction sector
Authors: Shah Mushtaq Ahmad , Ganesh Udaya Bhaskara 
Published: January 2025

Purpose
India’s construction sector faces substantial challenges with project delays and cost overruns. According to the Ministry of Statistics and Programme Implementation, an astounding 431 out of 1,820 projects reported cost overruns, whereas 848 projects experienced delays in 2023. These delays and overruns often push costs 30%–40% beyond initial estimates. Blockchain technology (BCT) offers a promising solution by enhancing transparency, streamlining processes through automation and optimising supply chains in the construction industry. This study aims to explore the adoption landscape of BCT within the construction sector, the drivers behind its acceptance and future directions for its implementation.

Design/methodology/approach
This study uses a mixed-methods’ research design that combines both quantitative and qualitative data collection and analysis techniques to investigate the current use of blockchain, the perceived rationale and the future direction of blockchain in construction. A survey of construction professionals with substantial blockchain experience was conducted. In addition, an exhaustive literature review and content analysis were conducted using reputable electronic databases such as Scopus, Web of Science and IEEE Xplore, reinforcing the study’s empirical foundation. The survey data was analysed using descriptive and inferential statistics to determine relative importance items using MS-Excel and SPSS.

Findings
Despite regulatory challenges, blockchain adoption is on the rise, with 90% of respondents expressing strong interest. Stakeholders see improved transparency and accountability as key drivers for adoption, emphasising blockchain’s capacity to support veracity and trust in construction projects. Notable benefits include enhanced communication, cost reduction, supply chain management and precise execution and monitoring. Overcoming regulatory constraints and bridging knowledge gaps will be essential to fully realising blockchain’s benefits for all players in the construction industry.

Originality/value
The study’s findings provide valuable insights for construction professionals, researchers and policymakers interested in adopting BCT. By clarifying the transformative potential of blockchain within the construction sector, this research study emphasises the imperatives of further study, innovation and collaborative endeavours aimed at addressing construction industry challenges and facilitating digital integration.
Wicked problems and value co-destruction in service ecosystems: navigating the tension between public and private value
Authors: Naveed Shabana , Farooqi Madiha Rehman , Salman Yaamina 
Published: January 2025

PurposeThis study explores the phenomenon of service value co-destruction within public service ecosystems (PSEs), focusing on the causes of value co-destruction and the inherent tension between public and private value creation/destruction.Design/methodology/approachQualitative data was collected through 36 semi-structured interviews with diverse stakeholders within the ecosystem of electricity services in Pakistan, including electricity suppliers, users and connected agencies involved in electricity generation, transmission, regulation, monitoring and coordination tasks.FindingsThe study identified various causes of service value co-destruction in PSEs, involving multiple actors responsible for value co-destruction. The research revealed the co-existence and intersection of public and private values within PSEs, highlighting the complex dynamics between value creation and destruction resulting in value imbalance and value erosion.Research limitations/implicationsValue co-destruction is a plausible outcome of dynamic interactions among various stakeholders in public service delivery, and it can have significant consequences on service quality and public value. Multiple stakeholders, in the governance network, can actively contribute to the co-destruction of service value.Practical implicationsPractitioners should consider the broader ecosystem where not only service providers but also service users and other connected actors, such as public, private and nonprofit organizations, collectively contribute to either co-creating or co-destroying service value.Originality/valueGrounded in PSL perspective, the findings deepen our understanding of the complexities surrounding public service delivery in PSEs. It provides a fresh perspective on how various actors collectively shape the value of these services.
When invisible eyes undermine proactive efforts: the effect of electronic performance monitoring on job crafting
Authors: Liu Wenxing , Zhou Kong , Ouyang Xi , Chen Siyuan , Gao Kai 
Published: January 2025

PurposeIn recent years, organizations have progressively adopted electronic performance monitoring (EPM) to obtain accurate employee performance data and improve management efficiency in response to the growing complexity of the work environment. However, existing research has primarily focused on examining the effect of EPM on employee behaviors within established job designs, neglecting the consequential role of EPM in shaping employees’ bottom-up job redesign (i.e. job crafting). This study aims to explore whether and how EPM affects employee job crafting.Design/methodology/approachTo test proposed hypotheses, we conducted two time-lagged surveys across different cultural contexts and a scenario experiment on an online platform in China.FindingsThe results revealed the negative indirect relationship between EPM and employee job crafting via role breadth self-efficacy. This indirect relationship was moderated by constructive supervisor feedback and job complexity, with the above relationships being weak (versus strong) when constructive supervisor feedback was high (versus low) or job complexity was low (versus high).Practical implicationsThe results have crucial implications for organizational practices, suggesting that managers should provide constructive feedback to break the trade-off between EPM and job crafting. Additionally, managers may need to give employees with high job complexity more autonomy rather than intense monitoring.Originality/valueThis study is the first to clarify the effect of EPM on employee job crafting. As job crafting captures the important value of employees in organizational job design, our effort helps to enrich the understanding of EPM effectiveness.
Competition–risk nexus of Taiwanese banks: from a stability–inefficiency perspective
Authors: Liao Chang-Sheng , Zhao ManJie 
Published: January 2025

PurposeThis study investigates the competition–risk nexus for Taiwan’s banking industry from the stability–inefficiency perspective over the period 2002–2021.Design/methodology/approachThis study investigates the effect of competition on bank risk from the perspective of stability inefficiency. Following Koetter et al. (2012), we measured bank competition levels by adjusting the Efficiency Lerner index.FindingsThis finding supports the competition–stability hypothesis for the banking sector, which states that intensified competition reduces banks’ insolvency and credit risk, making the banking sector sounder. However, banks enjoy a “quiet life” environment and face less competition but still obtain high profits despite inefficiency.Originality/valueThis study considers the moderating effects of multiple factors and finds that bank size, the revenue growth rate and new business income moderate the relationship between risk and competition. In addition, compared with the Z-score, this study investigates the competition–risk relationship through stability inefficiency, which was evaluated using the stochastic frontier approach, offering more robust results than previous studies.
Gifting a warm-glow effect: how identity influences charitable gifting
Authors: Ranga Garima , Khandeparkar Kapil , Motiani Manoj , Sharma Purvendu 
Published: January 2025

PurposeThis work explores how recipients’ identity influences givers’ choice of charitable gifts, their propensity to deviate from gifting registries and how it impacts social ties.Design/methodology/approachWe conducted five experiments involving diverse charities to explore the above effect, investigate the mediating role of a warm-glow effect and examine the moderating effect of social closeness and anonymity on gift preferences.FindingsGivers deviate from the registry in favor of alternatives that are congruent with the recipient’s identity due to the givers’ desire to gift a warm-glow effect. Socially close givers are more likely to deviate from the gift registry to enhance social ties. Recipients receive a higher warm-glow effect when distant givers give unrequested identity-congruous gifts, thus improving social relationships.Practical implicationsThis study will help organizations determine which identities a specific charity should highlight in its campaigns. Marketers can encourage consumers to prefer charitable gifts by emphasizing the relational and warm-glow benefits.Originality/valueThis is the first study to examine the effect of the recipient’s identity on givers’ preference for charitable gifts. Specifically, while giving charitable gifts, givers are likelier to donate to a charity whose identity is congruent with the recipient.