bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of the COVID‐19 pandemic on unmet healthcare needs after hospital discharge in acute respiratory failure survivors: A multicenter prospective cohort study
Authors: Carmichael Harris L. , Groat Danielle , Dinglas Victor D. , Bose Somnath , Kasimov Mustafa Mir , Jackson James C. , Kadiri Naga Preethi , Trejo‐Zambrano Maria Isabel , Sevin Carla M. , Green Tom , Needham Dale M. , Brown Samuel M. ,   for the APICS‐COVID Study Team
Published: December 2024

AbstractSurvivors of acute respiratory failure (ARF) have complex healthcare needs postdischarge, frequently resulting in unmet needs. This prospective multicenter study explores the association between COVID‐19 status and unmet healthcare needs in survivors of ARF following hospital discharge. We analyzed patient characteristics and unmet healthcare needs by COVID‐19 status using a multivariable regression model with propensity weights. Among 195 patients in the final analysis, 54% were COVID‐19+, 53% were female, 42% were non‐White, and the median (interquartile range [IQR]) age was 54 (42–63). The median (IQR) percentage of unmet healthcare needs was 26% (17%–36%), with follow‐up appointments accounting for most unmet needs. Unmet medication needs were found to be very low at 6%. Despite the challenges in healthcare delivery during the COVID‐19 pandemic, our data suggest COVID status had no observable effect on unmet healthcare needs for ARF survivors, after controlling for patient baseline and clinical status.
One‐Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point‐of‐Care Testing
Authors: Fan Zihao , Xu Ling , Cao Yaling , Liu Tianxu , Tian Yuan , Pan Zhenzhen , Mo Yinkang , Wang Xinyu , Zhu Xianru , Gao Yao , Zhang Xiangying , Pan Calvin Q. , Wang Lin , Ren Feng 
Published: December 2024

ABSTRACTHepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, and easy‐to‐use detection. This study aimed to develop a One‐Pot assay using CRISPR/Cas13a for detecting HEV RNA, suitable for point‐of‐care testing (POCT) in resource‐limited settings. CRISPR/Cas13a combined with reverse transcription polymerase chain reaction (RT‐PCR) and reverse transcription recombinase‐aided amplification (RT‐RAA) was applied to a One‐Pot assay device. Additionally, a large cohort of HEV‐infected patient (154) and animal (104) specimens was utilized for validation. The RT‐PCR/RT‐RAA + CRISPR/Cas13a assays for HEV RNA detection (genotypes: HEV‐1, HEV‐3, and HEV‐4) were established, optimized, and validated, achieving a limit of detection (LoD) of 1 copy/μL and 100% specificity. In the application validation for HEV infection, the positive rates of the RT‐PCR + CRISPR and RT‐RAA + CRISPR assays were 98.6% and 89.6% for patients, and 96.6% and 88.8% for animals, respectively, which were superior to those of RT‐qPCR. Furthermore, sample rapid lysis, reagent lyophilization, and the One‐Pot device were integrated to construct a One‐Pot assay with an LoD of 102 copies/μL. Despite slight decreases in sensitivity, the One‐Pot assay significantly reduces the assay time to 35 min, making it easy to perform, minimizing contamination, and meeting the requirements for screening. We developed a One‐Pot assay of HEV RNA using the CRISPR/Cas13a which effectively realizes a POCT test and maximizes the impetus for POCT implementation and shows potential as a valuable tool for detecting and monitoring HEV infection.
Long Noncoding RNA LINC02453 Inhibits HIV‐1 Replication by Binding With SEC13 to Regulate the Viral Productive Cycle
Authors: Chen Xiu , Chen Rongfeng , Wen Liufang , Qin Tongxue , Liao Yinlu , Tao Xing , Yuan Zongxiang , Wei Wudi , Li Jinmiao , Huang Youjin , Wei Wenfei , Liu Jie , Su Jinming , Ye Li , Liang Hao , Jiang Junjun 
Published: December 2024

ABSTRACTEmerging evidence underscores the pivotal role of long noncoding RNAs (lncRNAs) as crucial regulators within the HIV life cycle. However, the precise functions and detailed mechanisms by which lncRNAs operate in HIV‐1 highly exposed but persistently seronegative (HESN) individuals remain currently unknown. Through RNA sequencing analysis of the HESN individual and the matched control, we identified potential lncRNAs. Then, we conducted validation experiments at the population level, while cellular models of HIV‐1 infection were constructed for functional experimental investigations in vitro. Subcellular localization of the identified lncRNA was determined, followed by an exploration of the specific regulatory mechanism underlying HIV resistance through some experiments, such as RNA pull‐down, western blot and Hirt assays. LncRNA LINC02453 is highly expressed in HESN. Moreover, LINC02453 is identified as a novel lncRNA associated with heightened resistance to HIV‐1. LINC02453 is predominantly localized in the nucleus and binds to SEC13, a component of the nuclear pore complex, leading to the inhibition of HIV‐1 replication by regulating key processes such as late reverse transcription, nuclear import, and DNA integration. Our findings suggest that LINC02453 may serve as a prospective target for the development of innovative anti‐HIV therapeutics.
Preprocedural imaging guiding ventricular tachycardia ablation in structural heart disease
Authors: Nunes‐Ferreira Afonso , Brito Joana , Cortez‐Dias Nuno , da Lima da Silva Gustavo , Pinto Fausto J. , de Sousa João 
Published: December 2024

AbstractBackgroundIntegration of preprocedural imaging techniques in ventricular tachycardia (VT) ablation may improve the identification of arrhythmogenic substrates, particularly relevant for patients with nonischemic cardiomyopathy (NICM) with sub‐optimal outcomes. We assessed the impact of advanced preprocedural imaging on the safety and long‐term efficacy of radiofrequency catheter ablation (RCA) for VT, comparing patients with NICM and ischemic cardiomyopathy (ICM).MethodsIn this prospective, single‐center study, consecutive patients referred for scar‐related VT ablation underwent multidetector computed tomography (MDCT) and late gadolinium enhancement cardiac magnetic resonance (LGE‐CMR). Images were segmented with ADAS 3D software and integrated into mapping systems. Substrate map collection targeted the imaging‐predicted area of interest and the ablation aimed at eliminating all local abnormal ventricular activities. Procedural safety was evaluated with 30‐day mortality. Long‐term efficacy was assessed by survival free from appropriate ICD shocks at 36 months.Results102 patients were included (67 ± 11 years, 94% male; 75 ICM, 27 NICM). All patients underwent MDCT and 35% also underwent LGE‐CMR. Procedural safety (4% 30‐day mortality, p = .95) and 36‐month efficacy were similar in both groups (88.0% vs. 74.1%, HR 2.09; p = .13 in ICM and NICM). Efficacy was higher in patients when VT activation mapping with VT isthmus ablation complemented substrate ablation compared to substrate‐based ablation alone (94.5% vs. 80.6%, HR 4.00; p < .05).ConclusionA preprocedural imaging protocol integrated into the invasive mapping system may improve safety and long‐term efficacy, with NICM patients exhibiting outcomes comparable to those with ICM. Activation mapping of the VT on top of substrate ablation may improve prognosis.
Malevolent Creativity Graffiti Test—A New Measurement Method for Malevolent Creative Behavior
Authors: Tang Ruiyi , Li Qiangang , Gong Zhe 
Published: December 2024

ABSTRACTThis study introduces the malevolent creativity Graffiti test (MCGT), based on the Experimental Graffiti paradigm, as a measure of malevolent creative (MC) behavior. The malevolent creativity test (MCT) and the Malevolent Creativity Behavior Scale (MCBS) were used in two separate studies to assess the validity of the MCGT. Study 1 examined graffiti with a target of MC under two conditions: an imagined (MCGT1) and a recall (MCGT2) condition, involving 111 valid participants. Results revealed a significant positive correlation between MCGT1 and MCT, but no significant correlation with MCBS. MCGT2 showed no significant correlation with either MCT or MCBS. Study 2 explored graffiti without an MC target in a real competitive context (MCGT3) and graffiti with an MC target in a real provocative context (MCGT4), involving 126 valid participants. Both MCGT3 and MCGT4 demonstrated a significant positive correlation with MCBS. The associations between different types of MCGTs and established MC measures (i.e., MCT and MCBS) suggest that the MCGT holds promise as an effective tool for assessing MC behavior. This development of the MCGT addresses some limitations of current MC measurement methods and opens new avenues for research in malevolent creativity.
Successful use of transcutaneous parasacral stimulation in a patient with myelomeningocele and fecal incontinence: A case report
Authors: Bigliardi Roman , Riera‐Canales Claudia , Oviedo Adriana , Ortiz Gonzalo , Fernández Julian , Meduri Santiago , Birsa Patricio , Messere Gabriela , Saps Miguel 
Published: December 2024

AbstractMyelomeningocele is a complex congenital defect that affects the spinal cord and structures associated with it. These patients often have neurogenic bowel and bladder dysfunction with constipation, fecal and urinary incontinence. In addition to dietary modifications, laxatives, and enemas, therapies such as biofeedback and neurostimulation (invasive and noninvasive) are sometimes used. These techniques are rarely available, may require surgical procedures, and are not devoid of complications. We present the case of a 13‐year‐old boy with myelomeningocele at the L4 level, with constipation, dyssynergic defecation, and fecal incontinence (FI). He was treated with biofeedback achieving partial improvement of FI, and then with transcutaneous electrical neurostimulation (TENS) with subsequent resolution of constipation and FI. To our knowledge, this is the first case report using neurostimulation with TENS applied to the S2–S4 dermatomes in a patient with myelomeningocele. This represents a safe, noninvasive, and low‐cost treatment for these patients.
Polymeric Microneedles for Transdermal Delivery of Human Placental Tissue for the Treatment of Osteoarthritis
Authors: James Jithu Jerin , Pahal Suman , Jayaraman Anbu , Nayak A Damodar , Koteshwar Narasimhachar Sridhar , Sundarrajan Sudarson , Basappa Veerabhadraiah Basavaraj , Srinivasan Bharath , Vemula Praveen Kumar , Nguyen Thanh Duc , Kadamboor Veethil Sandhya 
Published: December 2024

AbstractBiologics targeting matrix‐degrading proteases, cartilage repair, and inflammation are emerging as promising approaches for osteoarthritis (OA) treatment. Recent research highlights biologic‐human placental tissue (HPT) as a potential OA therapy due to its biocompatibility, abundant protein biofactors, and ability to reduce cartilage degradation by suppressing protease expression. Microneedles (MNs) are receiving growing attention for enhancing transdermal delivery of biologics as an alternative to conventional subcutaneous injections. The lyophilized human placental extract (LHP) loaded polymeric MNs are fabricated using a micromolding technique for transdermal delivery. Ex vivo release studies reveal that MNs exhibit a gradual and consistent release of LHP, indicating a sustained delivery profile. LHP‐MNs are nontoxic and anti‐inflammatory in nature against human skin cells and interleukin (IL‐1β) induced synovial cells. Furthermore, the in vivo study shows that LHP‐MNs substantially improve behavioral parameters in OA rat models and lower serum concentrations of tumor necrosis factor‐ α (TNF‐α) and cartilage oligomeric matrix protein (COMP) biomarkers, thereby alleviating knee and ankle joint injuries. Histopathological analysis indicates that LHP‐MNs significantly preserve cartilage integrity. The study results suggest that employing polymeric MNs for transdermal delivery of LHP can be a promising treatment approach for OA, with the added benefit of excellent patient compliance.
Two‐parameter B‐valued martingale Hardy–Lorentz–Karamata spaces: Inequalities and dualities
Authors: Hao Zhiwei , Wang Lin , Weisz Ferenc 
Published: December 2024

AbstractIn this paper, we introduce five two‐parameter ‐valued martingale Hardy–Lorentz–Karamata spaces and establish some atomic decomposition theorems via atomic martingales as well as atomic functions. With the aid of atomic decompositions, we obtain some martingale inequalities and characterize the duals of these spaces. Our conclusions strongly depend on the geometric properties of the underlying Banach spaces and remove the restrictive condition that the slowly varying function is nondecreasing as in the previous literature.
Modeling of arcs with binary gas mixtures in a multi‐arc plasma generator
Authors: Bobzin K. , Heinemann H. , Erck M. , Warkentin S. , Mokrov O. , Sharma R. , Reisgen U. , Jasutyn K. 
Published: December 2024

AbstractUsing plasma as an energy source in thermal spraying allows the processing of feedstock materials with high melting temperatures. In plasma spraying, hydrogen or nitrogen is commonly injected into the argon plasma, which increases the specific enthalpy and thermal conductivity. The objective of this study is to improve the understanding of this process through numerical simulation and to investigate the impact on the plasma properties. A comprehensive modelling approach for the electrodes in the three‐cathode plasma generator is built upon the previous work of the authors. The plasma is described as an electromagnetically reactive fluid in local thermodynamic equilibrium using temperature‐dependent thermodynamic and transport properties. The increase of flow temperature and velocity as well as the temperature distribution at the electrodes can be successfully simulated. The simulation model could accelerate parameter development for new coating systems in the future. This study completes the simulation model for multi‐arc plasma spraying using binary gas mixtures.
Optimization of vacuum isothermal quenching process and microstructure analysis of die steel Cr12MoV
Authors: Hu S. D. , Meng Y. K. , Tang X. F. , Shao G. B. 
Published: December 2024

AbstractThis study first employed a vacuum isothermal gas quenching process to heat‐treat Cr12MoV in an attempt to achieve a mixed structure of martensite and lower bainite. Based on an analysis of the impact of different isothermal gas quenching process parameters on the properties and microstructure of Cr12MoV steel, with a focus on material impact toughness and Rockwell hardness, the particle swarm optimization method was used to optimize the vacuum gas quenching process parameters for Cr12MoV steel. Using the optimized process parameters, Cr12MoV steel was subjected to vacuum isothermal gas quenching treatment. Microstructure observations and phase analysis revealed that the post‐quenching structure consisted of an excellent combination of martensite and lower bainite dual‐phase structures, with reduced carbide content and even distribution. After two tempering processes, it was observed that the residual austenite content significantly decreased. The vacuum isothermal gas quenching‐tempering process effectively reduced material anisotropy and tool deformation.
Unlocking the Chemical Diversity of Plant Catharanthus roseus: Nuclear Magnetic Resonance Spectroscopy Approach
Authors: Gupta Upasna , Kavya M. P. , Jayalakshmi K. , Sinha Neeraj 
Published: December 2024

ABSTRACTCatharanthus roseus, also known as Madagascar periwinkle, is a perennial plant renowned for its extensive pharmacological properties. It produces vital chemotherapeutic compounds, including vinblastine and vincristine, and exhibits anti‐inflammatory, antidiabetic, and antioxidant activities. In this study, we utilized a range of two‐dimensional (2D) nuclear magnetic resonance (NMR) techniques, such as 1H–1H correlation spectroscopy (COSY), 1H–1H J‐resolved NMR, and 1H–13C heteronuclear single quantum coherence (HSQC) sensitivity‐enhanced NMR spectroscopy, to identify key metabolites in C. roseus leaf extracts. Given the presence of numerous metabolites with closely spaced multiplet resonances, the 1H NMR spectra often exhibit significant signal overlap, making metabolite identification difficult or even impossible. However, the use of 2D NMR techniques effectively overcame this challenge, allowing for the precise identification of important alkaloids, such as vindoline, vinblastine, serpentine, and ajmalicine, along with essential metabolites like organic acids, amino acids, and carbohydrates. The extract contained a variety of bioactive compounds, including organic acids crucial for the tricarboxylic acid (TCA) cycle, branched‐chain amino acids vital for metabolic functions, and alkaloids with substantial therapeutic potential. This comprehensive study underscores the continued significance of C. roseus in both traditional and modern medicine, emphasizing its intricate metabolic network and its potential in the development of novel therapeutics.
Deciphering Lobbying as a Public Affairs Strategy: Insights From Canadian Ministers and Political Advisors
Authors: Chan Ken 
Published: December 2024

ABSTRACTLobbying is a core element of a firm's public affairs strategy and can help it gain strategic advantages and improve performance. This study examines political actors' perceptions of lobbying's role in shaping public policy and influencing government decisions. Through elite interviews with 30 former ministers and senior political staff, this study seeks to understand why political actors engage with corporate lobbyists. The interviewees observed that businesses can help governments strengthen policymaking and test the real‐world applicability of proposed initiatives. These findings offer insights into how companies can effectively deploy lobbying resources, while scientifically validating commonly known best practices.
Rhabdoid Tumor of the Kidney and Soft Tissues: Results from National Wilms Tumor Study‐5 and Children's Oncology Group Study AREN0321
Authors: Geller James I. , Renfro Lindsay A. , Grundy Paul E. , Perlman Elizabeth J. , Kalapurakal John A. , Ehrlich Peter F. , Biegel Jackie , Huff Vicki , Warwick Anne B. , Paulino Arnold , Mullen Elizabeth A. , Daw Najat C. , Hoffer Fredric A. , Tochner Zelig , Gow Kenneth , Gratias Eric , Ward Deborah A. , Anderson James R. , Fernandez Conrad V. , Dome Jeffrey S. 
Published: December 2024

ABSTRACTPurposeNational Wilms Tumor Study‐5 (NWTS‐5) and AREN0321 evaluated the outcomes of children with rhabdoid tumor of the kidney (RTK) and malignant rhabdoid tumor of soft tissues (MRT).Patients and MethodsEligible patients with RTK were enrolled prospectively on NWTS‐5 (1995–2002) and treated with carboplatin and etoposide alternating with cyclophosphamide (Regimen RTK). Patients with RTK or MRT were enrolled on AREN0321 (2005–2012) and received vincristine, doxorubicin, and cyclophosphamide alternating with carboplatin, cyclophosphamide, and etoposide (Regimens UH‐1 or dose‐reduced Revised UH‐1). We report event‐free survival (EFS) and overall survival (OS) from each study.ResultsThirty patients received Regimen RTK on NWTS‐5; on AREN0321, 20 received UH‐1 and 19 received Revised UH‐1. Patient and disease characteristics were statistically similar between studies. Patients on AREN0321 had significantly improved EFS and OS compared to those on NWTS‐5 (4‐year EFS = 23.1% vs. 16.7%; p = 0.020; 4‐year OS = 30.6% vs. 20.0%; p = 0.014), mostly driven by patients with Stage I/II disease (p = 0.05). Median time to an event was 3.6 months on NWTS‐5 compared to 7.2 months on AREN0321. There were no differences in EFS or OS by revised versus original Regimen UH‐1 on AREN0321, or by renal versus extra‐renal primary disease when the studies were pooled.ConclusionsThe more intensive treatment regimen used on AREN0321 improved EFS and OS overall, a result driven by patients with Stage I/II disease. Despite this improvement, outcomes for patients with rhabdoid tumor remain unsatisfactory and there is a need for novel therapeutic strategies.
Modified G‐CSF/ATG‐Based Haploidentical Transplantation Protocol in Pediatric Primary Hemophagocytic Lymphohistiocytosis: A Long‐Term Follow‐Up Single‐Center Experience
Authors: Xiao Juan , Yang Xingcheng , Wu Nanhai , Fan Shifen , Liu Zhouyang , Jiang Fan , Chen Jiao , Wei Jia , Sun Yuan 
Published: December 2024

ABSTRACTBackgroundPrimary hemophagocytic lymphohistiocytosis (HLH) is a hyperinflammatory syndrome caused by immune dysregulation. Hematopoietic stem cell transplantation (HSCT) represents the only option for long‐term cure for primary HLH. However, only around 25% of patients have a fully HLA‐matched donor.MethodsIn this retrospective study, we analyzed 42 pediatric patients with primary HLH who underwent haplo‐SCT using the modified granulocyte colony‐stimulating factor (G‐CSF)/antithymocyte globulin (ATG)‐based protocol. The conditioning regimen included 300–600 mg/m2 etoposide (VP16), along with low doses of busulfan (Bu) (0.8–1.2 mg/kg every 6 hours on Days −8 to −6), cyclophosphamide (Cy) (10 mg/kg/day on Days −4 to −3), fludarabine (Flu) (30 mg/m2/day on Days −5 to −3), and ATG (8–9 mg/kg total dose on Days −5 to −2) to reduce complications.ResultsAll 42 patients achieved successful engraftment. Following a median follow‐up period of 48.7 months, 32 of the 42 patients remained alive and disease free. The 2‐year overall survival (OS) rate was 78.4%, and the 5‐year OS rate was 73.7%. The 2‐year failure‐free survival (FFS) rate was 71.3%, and the 5‐year FFS rate was 66.5%. Patients who achieved complete remission at the time of HSCT showed better OS (p < 0.05). The incidence of Grade III–IV acute graft‐versus‐host disease (GVHD) was 26.2%, and severe chronic GVHD was observed in 11.9% of patients. Thrombotic microangiopathy occurred in 13 patients, and veno‐occlusive disease in two patients.ConclusionsThis modified G‐CSF/ATG‐based haploidentical protocol demonstrates significant potential for pediatric patients with primary HLH, exhibiting commendable effectiveness and safety.
Multidimensional architecture of BaTiO3@Al2O3 nanofibers and BNNSs enables high energy storage performance of PVDF‐based composites
Authors: Deng Wei , Wang Zhao‐Pan , Xiong Heng , Hu Si‐Yuan , Cui Wei‐Wei 
Published: December 2024

AbstractPolymer‐based composites with high energy storage density are essential for flexible dielectric capacitors. Herein, a series of poly(vinylidene fluoride) (PVDF)‐based nanocomposite films are prepared by incorporating multidimensional hybrid fillers composed of BaTiO3 nanoparticles (BT NPs), Al2O3 nanofibers (Al2O3 NFs) and hexagonal boron nitride nanosheets (BNNSs), where BT NPs are embedded and homogeneously dispersed in Al2O3 NFs using electrospinning technique. Benefiting from Al2O3 and BNNSs with wide‐band gap and high thermal conductivity as well as BT with high dielectric constant, a prominent enhancement of dielectric constant, breakdown strength and thermal conductivity are achieved in BT@Al2O3‐BNNSs/PVDF nanocomposite. As a consequence, the energy storage density of BT@Al2O3‐BNNSs/PVDF nanocomposite reaches 13.1 J/cm3 at 490.9 kV/mm, which is 3.2 times that of PVDF and approximately 2 times that of PVDF nanocomposites using only BT@Al2O3 or BNNSs as fillers. This work highlights the synergistic effects of nanofillers with different characteristics, and provides a promising strategy for flexible dielectric materials towards energy storage application.Highlights
Designed multidimensional fillers containing BaTiO3 NPs, Al2O3 NFs, and BNNSs.
Co‐enhanced dielectric and thermal conductivity properties of PVDF composites.
Excellent energy storage density of 13.1 J/cm3.