bluuebunny/crossref_metadata_2025_split huggingface dataset:
Higher rate of covid-19 mortality in patients with type 1 than type 2 diabetes: a nationwide study
Authors: Demirci Ibrahim , Haymana Cem , Tasci Ilker , Satman Ilhan , Atmaca Aysegul , Sahin Mustafa , Ata Naim , Unluturk Ugur , Cakal Erman , Dagdelen Selcuk , Sahin Ibrahim , Celik Osman , Ertugrul Derun , Demir Tevfik , Emral Rifat , Caglayan Murat , Salman Serpil , Birinci Suayip , Sonmez Alper 
Published: August 2021

Abstract
PurposeCOVID-19 disease has a worse prognosis in patients with diabetes, but the comparative data about the course of COVID-19 in patients with type 1 (T1DM) and type 2 diabetes (T2DM) are lacking. The purpose of this study was to find out the relative clinical severity and mortality of COVID-19 patients with T1DM and T2DM.MethodsA nationwide retrospective cohort of patients with conﬁrmed (PCR positive) COVID-19 infection (n=149,671) was investigated. After exclusion of individuals with unspecified diabetes status, the adverse outcomes between patients with T1DM (n=163), T2DM (n=33,478) and the non-diabetics (n=115,108) were compared by using the propensity score matching method. The outcomes were hospitalization, the composite of intensive care unit (ICU) admission and/or mechanical ventilation and mortality.ResultsThe patients with T1DM had higher mortality than the age and gender matched patients with T2DM (n=489) and the non-diabetics (n=489) (p<0.001). After further adjustment for the A1c, microvascular and macrovascular complications, the odds of mortality (OR:3.35, 95% CI:1.41–7.96, p=0.006) and ICU admission and/or mechanical ventilation (OR: 2.95, 95% CI:1.28–6.77, p=0.011) were significantly higher in patients with T1DM compared to those with T2DM. Older age (OR:1.06, 95% CI:1.01–1.12, p=0.028) and lymphopenia (OR:5.13, 95% CI:1.04–25.5, p=0.045) were independently associated with mortality in patients with T1DM.ConclusionAccording to the results, patients with T1DM had poorer COVID-19 prognosis than those with T2DM or the non-diabetics. These patients should be cared diligently until more data will be available about the causes of increased COVID-19 mortality in T1DM.
Complete Home Smoking Ban Survey Analysis: An Opportunity to Improve Health Equity Among Sexual Minority Adults in California, USA
Authors: Boman-Davis Marie , Irvin Veronica L. , Westling Erika 
Published: August 2021

Abstract
Introduction: Increasing the proportion of adults living in smoke-free homes is a US Healthy People 2020 objective. Complete home smoking bans are associated with higher odds of smoking cessation attempts and cessation duration. Sexual minority adults have disproportionality higher rates of smoking. This study investigates correlates of having a complete home smoking ban among sexual minority adults in California. Methods: Secondary data analyses of the California Behavioral Risk Factor Surveillance System (CA BRFSS), 2014-2016. The CA BRFSS telephone survey of adults was conducted in English and Spanish and used random digit dial for landline and cell numbers. Weighted descriptive, bivariate, and multivariable logistic regression analyses were stratified by sexual orientation and biological sex.Results: Sexual minority adults in California had a lower prevalence of complete home smoking bans (Female 76.2%; Male 75.7%), higher prevalence of current cigarette smoking (Female 23.3%; Male 17.4%) and of e-cigarette use (Female 5.8%; Male 6.4%) than their straight counterparts. Sexual minorities that currently smoke “everyday” (Female AOR 0.26, 95% CI 0.11-0.63; Male AOR 0.24, 95% CI 0.01-0.56) or “somedays” (Female AOR 0.28, 95% CI 0.090.90) had lower adjusted odds of having a complete home smoking compared to those who “never smoked”. Conclusions: Currently smoking everyday was the strongest predictor of not having a complete home smoking ban among sexual minority adults. Focused efforts to increase prevalence of complete home smoking bans should address smoking status to improve health equity among sexual minority adults.
Application of deep metric learning to molecular similarity
Authors: Coupry Damien , Pogany Peter 
Published: August 2021

Abstract
Graph based methods are increasingly important in chemistry and drug discovery, with applications ranging from QSAR to molecular generation. Combining graph neural networks and deep metric learning concepts, we expose a framework for quantifying molecular similarity based on learned embeddings separate from any endpoint. Using a minimal definition of similarity, and data from the ZINC database of public compounds, this work demonstrate the properties of the embedding and its suitability for a range of applications, among them a novel reconstruction loss method for training deep molecular auto-encoders. We also compare the performance of the embedding to standard practices, with a focus on known failure points and edge cases.
Induced effect of Ca2+ and Al3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation
Authors: Liu Changqing , Chen Tianwen , Chen Jijie , Zhou Yuxuan , Deng Lina , Feng Gongneng , Gao Jian , Liang Huixing 
Published: August 2021

Abstract
Chaetominine (CHA), an alkaloid with biological activity produced by Aspergillus fumigatus CY018, has strong anticancer activity against the human leukemia cell. However, its further research for pharmacokinetics is limited by CHA production in submerged fermentation, which is a problem urgently awaiting solutions by effective biological strategies. In this work, Ca2+ and Al3+ exhibited a strong promoting effect on CHA production after multiple metal ions screening. Then, the concentration of Ca2+ and Al3+ and the addition time was optimized. The suitable conditions were 0.5 mM Ca2+ and 0.5 mM Al3+ at 120 h, in which the CHA production reached 58.5 and 49.45 mg/L with optimized condition of Ca2+ and Al3+ respectively. The intermediate metabolites, such as DAHP, chorismate and tryptophan, were increased obviously with addition of Ca2+ and Al3+. The activities of key enzymes of DAHPs, AroAs and TrpCs in CHA biosynthesis pathway were improved by 3.58-, 3.60- and 3.34-folds respectively. Meanwhile, the transcription levels of laeA, dahp, cs, and trpC were upregulated by 3.22-, 12.65-, 5.58- and 6.99-fold respectively by addition of Ca2+ and Al3+. Additionally, the fermentation strategy was successfully scaled up to 5 L reactor, in which CHA production could attain 75.6 mg/L at 336 h. This work demonstrated that Ca2+ and Al3+ co-addition was an effective strategy for increasing CHA production, and the information obtained might be useful to the further improvement of CHA fermentation on large scale.
18F-NOTA-FAPI-04 uptake in metastatic lesions on PET/CT imaging can distinguish different pathological types of lung cancer
Authors: Wei Yuchun , Cheng Kai , Fu Zheng , Zheng Jinsong , Mu Zhengshuai , Zhao Chenglong , Liu Xiaoli , Wang Shijie , Yu Jinming , Yuan Shuanghu 
Published: August 2021

Abstract
Purpose

Radionuclide-labeled fibroblast-activation-protein inhibitor 04 (FAPI-04) as a positron emission tomography (PET) imaging tracer can reveal the localized expression of fibroblast activation protein (FAP), a cell-surface serine protease that is highly upregulated in more than 90% of epithelial carcinomas. In this study, we quantified the 18F-NOTA-FAPI-04 uptake on PET/computed tomography (CT) imaging in different pathological types of lung cancer and metastatic tumors.
Methods

We prospectively enrolled 61 patients with histopathologically proven primary lung cancer with metastases.PET/CT scanning was performed before any antitumor therapy and 1hour after injection of 235.10 ± 3.89 MBq of 18F-NOTA-FAPI-04. Maximum standard uptake values (SUVmax) were calculated for comparison among primary and metastatic lesions.
Results

Sixty-one patients with adenocarcinoma (ADC, n = 30), squamous cell carcinoma (SCC, n = 17), and small cell lung cancer (SCLC, n = 14) were enrolled in this study, and 61 primary tumors and 199 metastases were evaluated. No difference in18F-NOTA-FAPI-04 uptake was observed among primary ADC, SCC, and SCLC tumors (P = 0.198).Additionally, no difference in uptake was found between primary and metastatic lesions of lung cancer with the same pathological type (P > 0.05).However, uptake did differ among metastases of differing pathological type (P < 0.001). The SUVmax of metastatic lymph nodes was highest for SCC, followed by ADC and then SCLC (P < 0.001).The SUVmax of bone metastases also was highest for SCC, followed by ADC and SCLC (P < 0.05), but no difference was observed between ADC and SCLC. The SUVmax of metastases in other organs was higher in SCC cases than in ADC cases, but did not differ between SCC and SCLC or ADC and SCLC. Bone metastases exhibited higher uptake than those of lymph nodes and other organs in SCC and ADC (P < 0.05), but not in SCLC.
Conclusion

18F-NOTA-FAPI-04 PET/CT imaging revealed differences in FAP expression in metastases of lung cancer, with the highest expression specifically in bone metastases, and thus, may be valuable for distinguishing different pathological types of lung cancer.
Hepatitis B Virus Demethylates PD-1 and Modulates the Changes of the Tumor Microenvironment in Hepatocellular Carcinoma Patients
Authors: Lian Weiguang , Lai Ruixue , Wu Jianhua , Zhang Jingjing , Li Shengchao , Peng Li , Guo Zhanjun 
Published: August 2021

Abstract
Background: Hepatitis B virus (HBV) constitutes a major global health burden. Previously study has found the expression of programmed cell death 1 (PD-1) is up-regulated during the chronic HBV infection. However, the mechanism of how HBV infection modulates the expression of PD-1 on CD8+T cells are not well understood. In the present study, we aimed to analyzed the role of DNA methylation in regulating the expression of PD-1 on CD8+T cells during HBV infection, we also aimed to evaluate the HBV induced changes of tumor microenvironment (TME).Methods: The methylation microarray was used to assess the profile changes of gene methylation upon chronic HBV infection. CD8+T cells were separated from peripheral blood of health volunteers and HCC patients including HBV related HCC (HBV-HCC) and non-viral HCC. The immune microenvironments of hepatocellular carcinoma (HCC) was interrogated by using Immunofluorescence staining. The PD-1 expression of CD8+T cells from peripheral blood were examined by western blot and flow cytometry. T cells function was determined by cytokine measurement. Sequenom MassARRAY platform was used to detected the DNA methylation status of PD-1 promoter.Results: HBV could drive 413 genes methylated while 3,023 genes including PD-1 demethylated, the reduced PD-1 expression and increased PD-1 demethylation was proved in HBV-HCC tissue. The subsequent analysis indicate that the expression of PD-1 differed by stages of HBV infection, it reduced on the cell membrane of HBV-transfected CD8+T cells upon transient HBV plasmid transfection which might mimic acute HBV infection, while upregulated on the HBV specific CD8+T cells by demethylation during chronic HBV infection. Interferon-gamma increased in the medium of HBV transfected CD8+T cells and interleukin-10 increased in the blood of chronic HBV infection patients. Increased tumor associated macrophage (TAM) cells and T regulatory cells (Tregs) invasion was identified in HBV-HCC tissue when compared with those from non-viral HCC tissue Conclusion: Our data suggested that the expression of PD-1 varies upon the stage of HBV infection, chronic HBV infection could drove the TME more immunosuppressive through PD-1 demethylation related CD8+T cells exhaustion as well as promotion of invaded Treg and TAM cells thereby to attenuating therapy of immune checkpoint inhibitors.
Molecular Docking Prediction and in Vitro Studies Elucidate Anti-inflammatory Effect of Garcinia Extract Against Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Targets
Authors: Kalita Anuradha , DAS MANAS , Das Bhabajyoti , Baro Momita Rani 
Published: August 2021

Abstract
Garcinia is a tropical plant that has been traditionally used in medicinal folklore for its potential antioxidant, antibacterial, anti-hyperlipidemic, anti-diabetic, hepatoprotective, etc. In this study, Garcinia herbal extract (GHE) and one of its important phytocompound (garcinol) were evaluated for their inhibitory action against important inflammatory markers inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. iNOS and COX-2 plays an major role in the process of inflammation and inhibition of these molecules will help to alleviate the inflammatory process. The cells were pre-treated with two doses of Garcinia (230µg/ml and 115µg/ml); garcinol (12µM and 6µM) followed by stimulation with 1µg/ml of LPS for 24h. The results of the study demonstrated that GHE and garcinol plays an important role in suppressing LPS- induced relative mRNA expression of iNOS, COX-2 and subsequent reduction in the levels of nitric oxide and prostaglandin E 2 . Molecular docking analysis of garcinol and hydroxycitric acid, the major active components of GHE with iNOS and COX-2 proteins showed potent interaction with low binding energies. This study suggests that GHE (containing high percentage of HCA) and garcinol may possess anti-inflammatory activity thus providing a possibility for drug designing as iNOS and COX-2 inhibitors.
Characterization of Al-Mg-Si Alloy Reinforced with Optimum Palm Kernel Shell Ash (PKSA) Particle and its Consequence on the Dynamic Properties for Aerospace Application
Authors: Oyedeji Oyewusi Elijah , Dauda Muhammed , Yaro Shehu Aliyu , Abdulwahab Malik 
Published: August 2021

Abstract
Nowadays, the demand for naturally and locally sourced materials for usage in automobile and aerospace have been on the increase due to high usage and population increase. In this view, this research is focused on investigating aluminium metal matrix composite materials with the aim of studying the optimum composition effect of Al-Mg-Si alloy reinforced with Palm Kernel Shell Ash (PKSA) particle on static and dynamic properties. Powder methodology was used to manufacture the developed composites. The SEM result showed that the recrystallization during ball milling of the mixture powders characterized the accumulation of higher dislocation density and the presence of harder phases in the PKSA which contributed to their improved thermal properties. Furthermore, the modulus of rupture of the developed Al-Mg-Si-PKSA matrix composites was effectively increased, and it can be drawn that sample C4 (6:94 wt%) has optimized properties. In this study, the storage modulus (E’), loss modulus (E'), damping factor (tan δ) were determined based on the Dynamic Mechanical Thermal Analysis (DMTA) of the developed composites. It was established that the glass transition temperature (Tg) increased with frequency and amplitude. The result of this research also revealed the optimum percentage composition of 6 wt% PKSA on Al-Mg-Si powder is suitable for research aerospace applications.
Expression of TLR10 in B cells Correlates With Primary Sjögren’s Syndrome Progression
Authors: Lai Nannan , Qian YiChao , Wu Yilin , Jiang Xi , Sun Honghua , Luo Zhaofan , Zhao Yanli , Zeng Changchun , Kang Yang , Zhan Xiao-Yong , Wang Qingwen , Tang Chun , Huang Bihui 
Published: August 2021

Abstract
Primary Sjögren’s Syndrome (pSS) is considered a B cell-mediated disease, yet the precise role of B cells in the pathogenesis is not fully understood. Toll-like receptor 10 (TLR10) is highly expressed in human B cells, indicating that TLR10 probably plays a vital role in regulating B cell function as well as B cell-related diseases. However, the biology of TLR10 in pSS was rarely researched. Here, we examined the TLR10 expression in peripheral B cell subsets isolated from both pSS patients and healthy controls (HCs) and further analyzed the correlations between TLR10 expression and disease activity. We observed that TLR10 expression in peripheral total CD19+ B cells, naïve B cells (CD19+CD27-IgD+) and switched memory B cells (CD19+CD27+IgD-) was significantly increased in low-activity pSS patients as compared with HCs and high-activity pSS patients. TLR10 expression in total and switched memory B cells in pSS patients was significantly negatively correlated with serum levels of anti-SSA antibody and B cell activating factor of TNF family (BAFF). As compared with the TLR10 low-, the TLR10 high-expressed pSS patients presented with reduced switched memory B cells. Moreover, a much lower proportion of high-activity pSS patients was observed in TLR10 high- as compared to low-expressed patients. Our study concluded that TLR10 expression in peripheral total and switched memory B is negatively correlated with pSS disease activity, suggesting that TLR10 might suppress pSS progression via inhibiting the B cell class switch recombination. These results should contribute to the diagnosis and treatment of pSS.
Association Between the Alterations in the Blood Routine Check Parameters and Cognitive Impairment in the Shenzhen Ageing-related Disorder Cohort in China
Authors: Wu Kaiyu , Xu Chunyan , Tian Xiaoping , Qiu Guozhen , Liu Wei , Guo Qiwen , Meng Xueya , Chen Chunchun , Liu Jianjun , Guo Zhiwei , Chen Haifei , Liu Kangding , Zhu Feiqi 
Published: August 2021

Abstract
Background: Previous studies have suggested a correlation between alterations in the routine blood check markers and cognitive impairment. Objective: We aimed to explore the relationship between routine blood parameters and cognitive impairment. Methods: In this retrospective case-control study, 8,025 people over 60 years of age were enrolled from 51 community health centers in Luohu District of Shenzhen City from 2017 to 2018. According to the mini-mental state examination (MMSE) score and educational level, participants were divided into a cognitive impairment group (n=385) and normal cognitive function group (n=7,640). Nonparametric test, chi-square test, and binary logistic regression were applied to analyze the data. Results: Compared to the normal cognitive function group, the lymphocyte-to-monocyte ratio (LMR), packed-cell volume (PCV), and platelet count (PLT) in the cognitive impairment group were significantly lower (7.26 [5.50,8.81] vs. 7.45 [5.80,9.35]; 40.1 [36.65,42.95] vs. 40.5 [37.7,43.1]; 216 [190,258] vs. 228 [196,261], p<0.05, respectively), while the monocyte count and mean corpuscular hemoglobin concentration (MCHC) were higher (0.31 [0.25, 0.38] vs. 0.3 [0.24, 0.37]; 336 [330, 343] vs. 335 [328, 342], p<0.05, respectively). Additionally, MCHC [odds ratio (OR):1.011, 95% conﬁdence interval (CI) :1.001–1.021, p = 0.033] and PCV [OR: 0.993, 95% CI: 0.987–0.999, p = 0.033) were independently associated with cognitive impairment. Conclusion: The alterations in some routine blood check parameters, including reduced LMR, PCV, and PLT, and elevated monocyte count and MCHC, may be associated with cognitive impairment.
Temporal Trend of Comorbidity and Increasing Impacts on Mortality, Length of Stay, and Hospital Costs of First Stroke in Tianjin, North of China
Authors: Hao Ruixiao , Qi Xuemei , Xia Xiaoshuang , Wang Lin , Li Xin 
Published: August 2021

Abstract
Purpose: Stroke patients have a high incidence of comorbidity. Our study aimed to explore the trend of comorbidity among patients with first stroke from 2010 to 2020, and the influence of comorbidity on admission mortality, length of stay and hospitalization costs.5988 eligible patients were enrolled in our study, and divided into 4 comorbidity burden groups according to Charlson comorbidity index (CCI): none, moderate, severe, very severe. Survival analysis was expressed by Kaplan - Meier curve. Cox regression model was used to analyze the effect of comorbidity on 7-day and in-hospital mortality. Generalized linear model (GLM) was used to analyze the association between comorbidity and hospitalization days and cost. Results: Compared to patients without comorbidity, those with very severe comorbidity were more likely to be male (342, 57.7%), suffer from ischemic stroke (565, 95.3%), afford higher expense (Midian, 19339.3RMB, IQR13020.7-27485.9RMB), and have a higher in-hospital mortality (60, 10.1%). From 2010 to 2020, proportion of patients with severe and very severe comorbidity increased 12.9%. The heaviest comorbidity burden increased the risk of 7-day mortality (adjusted hazard ratio, 3.51, 95% CI, 2.22-5.53) and in-hospital mortality (adjusted hazard ratio, 3.83, 95% CI, 2.70-5.45). Patients with very severe comorbidity had a 12% longer LOS and extra 27% expense than those without comorbidity.Conclusion: Comorbidity burden showed an increasing trend year in past eleven years. The heavy comorbidity burden increased in-hospital mortality, LOS, and hospitalization cost, especially in patients aged 55 years or more.
Altered Glycolytic Capacity, Not Mitochondrial Common Deletion, May Be Responsible For Age-Related Increases in Rat Intestinal Permeability
Authors: Kundaktepe Berrin Papila , KARIMOVA AYLA , Onaran Ilhan 
Published: August 2021

Abstract
Some evidence points to a link between aging-related increased intestinal permeability and mitochondrial dysfunction in in-vivo models. Several studies have also demonstrated age-related accumulation of the of specific deletion 4834-bp of “common” mitochondrial DNA (mtDNA) in various rat tissues and suggest that this deletion may disrupt mitochondrial metabolism. The present study aimed to investigate possible associations among the mtDNA common deletion, mitochondrial function, intestinal permeability, and aging in rats. Our data showed were no significant differences in the abundance of mtDNA4834 deletions in intestinal tissue between young adult ((4-month-old) and aged (24-month-old) rats). In addition, Spearman's correlation coefficients of measured parameters in serum samples for intestinal permeability did not correlate with deletion frequency and measured levels of mitochondrial energy function parameters. However, the tissue lactate/pyruvate ratio (L/P) was three times lower in old rats than in young rats. Additionally, there were significant negative correlations between intestinal permeability parameters and L/P ratios. Considering our findings showing that the intestinal tissues of aged rats are not prone to accumulate mtDNA common deletion, we suggest that this mutation does not explain the age-related increase in intestinal permeability. Some data support the thought that altered glycolytic capacity could be a possible mechanism linked to increased intestinal permeability with age.