bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hoja de servicios y futuro de la sociolingüística catalana: una exploración epistemológica (y glotopolítica)
Authors: Boyer Henri 
Published: March 2021

Este texto pretende ser una exploración de la historia reciente de la sociolingüística en Cataluña desde su constitución como grupo en los años 70 y de su impacto glotopolítico hasta las controversias actuales frente a la perspectiva independentista.De tipo claramente nacionalista en sus inicios, fue más que una simple ciencia social: la inspiradora de la reconquista sociolingüística post-franquista a través de sus recomendaciones y de sus acciones que el poder autónomo de Cataluña ha sabido tener en cuenta.Actualmente parece desgarrarse en lo que se refiere al diagnóstico sociolingüístico y al objetivo glotopolítico entre una opción que permanece firme en cuanto a las bases otriginales: el catalán es la única lengua nacional de Catalunya y debe tener la por lo tanto exclusividad en una eventual República catalana; y otra opción que considera que el bilingüismo catalán-castellano tiene toda su razón de ser en una Cataluña independiente.
The impact of LED lightning on the content of photosynthetic pigments in tomato leaves
Authors: Nikanovich T. V. , Trofimov Yu. V. , Barkun M. I. 
Published: March 2021

Relevance and methods. We studied the influence of LED lighting of different spectral composition on the content of photosynthetic pigments in tomato leaves to identify the most optimal lighting option suitable for obtaining high-quality seedlings under controlled conditions. We used LED lamps in which the ratio of the photon flux density (PFD) of the orange-red band (607–694 nm) to the PFD of the blue band (400–495 nm) varied from 1 to 20. In this case, the proportion of the PFD in the range 580-607 nm ( yellow) ranged from 13 to 22%, and the fraction of photons in the range 495–580 nm (green) ranged from 18 to 38%. The research was carried out with two varieties of Belarusian tomato varieties, which differed in a number of morphobiological characteristics.Results. It was found that the use of LED lighting of different spectral composition had mainly an inhibitory effect on the biosynthesis of chlorophylls and carotenoids in the leaf tissue of plants. The decrease in the amount of pigments, in comparison with the control variant, reached 47-57%. It was revealed that under all studied lighting options, with the exception of conditions where the spectral ratio R / B ("red/blue") was 0.8, the value of the total inhibitory effect in the Cherry Coral variety was 1.2-1.7 times lower than that of the Zorka variety, which indicated a significantly lower susceptibility of the pigment fund of the former to LED lighting. The smallest inhibitory effect of the latter on the biosynthesis of photosynthetic pigments in both tomato varieties was established at a photon flux of 69.1 μmol/s, while the greatest, exceeding it by 3.0-3.1 times in the Zorka variety and 4.5-5.3 times for the Cherry Coral variety with a photon flux of 73.9 μmol/s.
Germination and Initial Growth of Sunflower Irrigated With Untreated and Treated (by Distillation and Electro-fenton) Landfill Leachate
Authors: Rigo Michelle Machado , Souza Paulo Sérgio , Cerqueira Alexandre Andrade , Paiva Julieta Laudelina de , Perez Daniel Vidal , Marques Monica Regina da Costa 
Published: March 2021

Abstract
Currently, water scarcity, especially in irrigated agriculture, has led researchers to study reuse to study industrial and sewage reuse. Landfill leachates deserve special attention due to its difficulty of tratment they are effluents that are difficult to treat and because it contains essential susbstances for plant nutrition, such as nitrogen, phosphorus and potassium and its application in agriculture can be considered a noble use if it is properly handled and does not cause phytotoxicity. This study investigated the effects of different dilutions of treated and non-treated landfill leachate on germination and vitality of sunflower seed (cv. BRS 321), its effects in growth,and vitality and soil atributes under sunflower culture in greenhouse. The leachate was treated by two electro-Fenton process or distillation. The results showed that sunflower cv. BRS 321 seeds, germinated vigorously up to 25% effluent dilution, showing resistance to salinity and phytotoxicity at 50% raw leachate dilution. 15% dillution showed satisfactory results in sunflower growth and nutrition. However the leachate use of higher concentrations showed some changes in soil characteristics. This study suggests that landfill leachate can be used in seed germination under proper use and application, in their lowest leachate concentrations and always based on the chemical analyzes of the leachate.
Microbiomes of Clownfish and Their Symbiotic Host Anemone Converge Before Their First Physical Contact
Authors: Audet-Gilbert Émie , Sylvain François-Étienne , Bouslama Sidki , Derome Nicolas 
Published: March 2021

Abstract
BackgroundOne of the most charismatic, and yet not completely resolved example of mutualistic interaction is the partnership of clownfish and its symbiotic sea anemone. The mechanism explaining this tolerance currently relies on the molecular mimicry of clownfish epithelial mucus, which could serve as camouflage, preventing the anemone's nematocysts' discharge. Resident bacteria are known as key drivers of epithelial mucus chemical signature in vertebrates. A recent study has proposed a restructuration of the skin microbiota in a generalist clown fish when first contacting its symbiotic anemone. We explored a novel hypothesis by testing the effect of remote interaction on epithelial microbiota restructuration in both partners. MethodsWith metataxonomics, we investigated the epithelial microbiota dynamic of 18 pairs of percula clownfish (Amphiprion percula) and their symbiotic anemone Heteractis magnifica in remote interaction, physical interaction and control groups for both partners during a four weeks trial.ResultsPhysical and Remote Interaction groups’ results evidence gradual epithelial microbiota convergence between both partners when fish and anemone were placed in the same water system. This convergence occurred preceding any physical contact between partners, and was maintained during the two-weeks interaction period in both contact groups. After the interaction period, community structure of both fish and anemone’s epthelial community structures maintained the interaction signature two weeks after fish-anemone pairs separation. Furthermore, the interaction signature persistence was observed both in Physical and Remote Interaction groups, thus suggesting that water-mediated chemical communication between symbiotic partners was strong enough to shift the skin microbiota durably, even after the separation of fish-anemone pairs. Finally, our results suggest that fish-anemone convergent microbiota restructuration was increasingly associated with the parallel recruitment of three Flavobacteriaceae strains closely related to a tyrosinase-producing Cellulophaga tyrosinoxydans. ConclusionsOur study shows that bacterial community restructuration, in the acclimation process, does not only rely on direct physical contact. Furthermore, our results challenge, for the first time, the traditional unidirectional chemical camouflage hypothesis, as we argue that convergence of the epithelial microbiota of both partners may play essential roles in establishing mutual acceptance.
Combined Effect of White Blood Cell and Platelet Count for Predicting In-Hospital Mortality and Pneumonia in Acute Ischemic Stroke
Authors: You Shoujiang , Xu Guoli , Zhou Yi , Zhong Chongke , Cheng Juping , Zhai Wanqing , Cao Yongjun , Liu Chun-Feng 
Published: March 2021

Abstract
Background: High white blood cell (WBC) count was the risk factors for mortality and pneumonia after acute ischemic stroke (AIS). Low platelet count increased the risk of mortality. We investigated the combined effect of WBC count and platelet count on hospital admission and in-hospital mortality and pneumonia in acute AIS patients.Methods: A total of 3,265 AIS patients enrolled from December 2013 to May 2014 across 22 hospitals in Suzhou city were included in the present study. We divided patients into four groups according to their level of WBC count and platelet count: LWHP (low WBC and high platelet), LWLP (low WBC and low platelet), HWHP (high WBC and high platelet) and HWLP (high WBC and low platelet). Cox proportional logistic regression model were used to estimate the combined effect of WBC count and platelet count on all cause in-hospital mortality and pneumonia in AIS patients. Results: HWLP was associated with a 2.07-fold increase in the risk of in-hospital mortality in comparison to LWHP (adjusted odds ratio [OR] 2.07; 95% confidence interval [CI], 1.02-4.18; P-trend =0.020). The risk of pneumonia was significantly higher in patients with HWLP compared to those with LWHP (adjusted OR 2.29; 95% CI, 1.57-3.35; P-trend <0.001). The C-statistic for the combined WBC count and platelet count was higher than WBC count or platelet count alone for prediction of in-hospital mortality and pneumonia (all p < 0.01). Conclusions: High WBC count combined with low platelet count level at admission was independently associated with in-hospital mortality and pneumonia in AIS patients. Moreover, the combination of WBC count and platelet count level appeared to be a better predictor than WBC count or platelet count alone.
Status of Exclusive Breast Feeding Among Children Born to Human Immunodeficiency Virus&nbsp;(HIV) Positive Mothers Attending Public Health Facilities in Western Ethiopia. Cross-Sectional Study.
Authors: Bultum Ejigayehu Tolessa , Roro Elias Merdassa , Regassa Ilili Feyesa 
Published: March 2021

Abstract
Background: Only about 39% of infants in the developing countries are exclusively breast-fed for the first six months. Human immunodeficiency virus (HIV) positive women were confused about feeding methods. Exclusive Breastfeeding (EBF) practice of Human immunodeficiency virus (HIV) positive mothers is sub-optimal in Ethiopia. Hence, we want to identify the main factors influencing exclusive breast-feeding among HIV positive breast-feeding mothers. Main aims of the study were to assess the level and factors influencing Exclusive Breastfeeding (EBF) among children born to Human Immunodeficiency Virus (HIV) positive mothers attending public health facilities in western Ethiopia.Methods: A facility based cross sectional study was carried out from September 2017 to June 30, 2018 among Human Immunodeficiency virus (HIV) positive mothers with infants aged 6-23 months. Among public health facilities found in three districts from of West, East and Kellem Wollega Zones; thirteen facilities (i.e. 7 health centers and 6 hospitals) were randomly selected from among proving ART and PMTCT Services. Respondents were recruited by systematic random sampling techniques from these facilities using clients registers as sampling frame. Data were collected using face to face interviewer administered pre-tested questionnaire. The data were entered into computers using EPI info Version 3.5.1 and analyzed with SPSS Version 20 for windows. Candidate variables for the final multi-variable model were selected considering P< 0.05 at bivariable analysis. Associations were declared at P< 0.05 by assuming Confidence Intervals did not crossed number ‘1’with corresponding 95%. Results were presented using standard data presentation tools. Results: A total of 218 Human Immune Virus positive mothers were included in this study. Of these, only 122 (56.0%) practiced Exclusive Breast Feeding. The proportion of respondents who initiated Exclusive Breast Feeding within the first hours of delivery were 134 (61.8%). Mean age of the study participants were 28.68 with SD + 4.2. Mothers’ advices on child exclusive breast-feeding [AOR 3, 95% CI (1.2-6.7)], disclosure of HIV status to close friends [AOR 6, 95% CI (1.18-29.64)] and believing HIV can be transmitted during delivery [AOR 5.2, 95% CI (1.10-24.00)] were found to increase the change of exclusive breast-feeding practices among the study participants (P-value < 0.05).Conclusion Slightly more than half of the mothers practiced exclusive breast feeding for the first six months. Care providers should encourage mothers to practice exclusive breast feeding in the first six months and to disclose their test results to their husbands. Efforts should be in place to curb the risk of HIV/AIDS transmission during delivery.
Optimization of Protocols for Pre-Embedding Immunogold Electron Microscopy of Neurons in Cell Cultures and Brains
Authors: Tao-Cheng Jung-Hwa , Crocker Virginia , Moreira Sandra Lara , Azzam Rita 
Published: March 2021

Abstract
Immunogold labeling allows localization of proteins at the electron microscopy (EM) level of resolution, and quantification of signals. The present paper summarizes methodological issues and experiences gained from studies on the distribution of synaptic and other neuron-specific proteins in cell cultures and brain tissues via a pre-embedding method. An optimal protocol includes careful determination of a fixation condition for any particular antibody, a well-planned tissue processing procedure, and a strict evaluation of the credibility of the labeling. Here, tips and caveats on different steps of the sample preparation protocol are illustrated with examples. A good starting condition for EM-compatible fixation and permeabilization is 4% paraformaldehyde in PBS for 30 min at room temperature, followed by 30 min incubation with 0.1% saponin. An optimal condition can then be readjusted for each particular antibody. Each lot of the secondary antibody (conjugated with a 1.4 nm small gold particle) needs to be evaluated against known standards for labeling efficiency. Silver enhancement is required to make the small gold visible, and quality of the silver-enhanced signals can be affected by subsequent steps of osmium tetroxide treatment, uranyl acetate en bloc staining, and by detergent or ethanol used to clean the diamond knife for cutting thin sections. Most importantly, verification of signals requires understanding of the protein of interest in order to validate for correct localization of antibodies at expected epitopes on particular organelles, and quantification of signals needs to take into consideration the penetration gradient of reagents and clumping of secondary antibodies.
Functional annotation of creeping bentgrass protein sequences based on convolutional neural network
Authors: Jiang Han-Yu , He Jun 
Published: March 2021

Abstract
Background: Creeping bentgrass (Agrostis soionifera) is a perennial grass of Gramineae, belonging to cold season turfgrass, but has shallow adventitious roots, poor disease-resistance. Little is known about the ISR mechanism of turfgrass and the signal transduction involved in disease-resistance induction, especially the function of a large number of disease-resistance related proteins are urgent to be explored.Results: In this work, the protein sequences of creeping bentgrass were measured and annotated by a functional prediction model based on convolutional neural network. Creeping bentgrass seedlings were grown with BDO treatment, and the ISR response was induced by infecting Rhizoctonia solani. We preformed the transcriptome analysis by Illumina Sequencing and high-quality unigenes were obtained. A minority of assembled unigenes were functionally annotated according to the database alignment while a large part of the obtained amino acid sequences was left non-annotated. To treat the non-annotated sequences, a prediction model was established by training the data set from GO families in three domains to acquire good performance, especially the higher false positive control rate. With such model, we analyzed the non-annotated protein sequences of creeping bentgrass transcriptome, and annotated the disease-resistance response and signal transduction related proteins. Conclusions: The results provide good candidates of the proteins with certain functions. With the results in this work, the waste of transcriptome sequencing data of creeping bentgrass can be avoided, and research time and labor for the analysis of ISR characteristics of creeping bentgrass will be saved in further research. It also provides reference for the sequence analysis of turfgrass disease-resistance research.
Convalescent plasma for adults with acute COVID-19 respiratory illness (CONCOR-1): Study protocol for an international, multicenter, randomized, open-label trial
Authors: Bégin Philippe , Callum Jeannie , Heddle Nancy , Cook Richard , Zeller Michelle P , Tinmouth Alan , Fergusson Dean , Cushing Melissa M , Glesby Marshall J , Chassé Michaël , Devine Dana V , Robitaille Nancy , Bazin Renée , Shehata Nadine , Finzi Andrés , McGeer Allison , Scales Damon , Schwartz Lisa , Turgeon Alexis F , Zarychanski Ryan , Daneman Nick , Carl Richard , Amorim Luiz , Gabe Caroline , Ellis Martin , Jamula Erin , Carruthers Julie , Duncan Joanne , Lucier Kayla , Armali Chantal , Kron Amie , Modi Dimpy , Auclair Marie-Christine , Avram Meda , Arnold Donald M 
Published: March 2021

Abstract
Background: Convalescent plasma has been used for numerous viral diseases including influenza, severe acute respiratory syndrome, Middle East respiratory syndrome and Ebola virus; however, evidence to support its use is weak. SARS-CoV-2 is a novel coronavirus responsible for the 2019 global pandemic of COVID-19 community acquired pneumonia. We have undertaken a randomized controlled trial to assess the efficacy and safety of COVID-19 convalescent plasma (CCP) in patients with SARS-CoV-2 infection.Methods: CONCOR-1 is an open-label, multicenter, randomized trial. Inclusion criteria include: patients >16 years; admitted to hospital with COVID-19 infection; receiving supplemental oxygen for respiratory complications of COVID-19; and, availability of blood group compatible CCP. Exclusion criteria are: onset of respiratory symptoms more than 12 days prior to randomization; intubated or planned for intubation; and previous severe reactions to plasma. Consenting patients will be randomized 2:1 to receive either approximately 500 mL of CCP or standard of care. CCP will be collected from donors who have recovered from COVID-19 and who have detectable anti-SARS-CoV-2 antibodies quantified serologically. The primary outcome is intubation or death at Day 30. Secondary outcomes include ventilator free days, length of stay in intensive care or hospital, transfusion reactions, serious adverse events, and reduction in SARS-CoV-2 viral load. Exploratory analyses include patients who received CCP containing high titre antibodies. A sample size of 1200 patients gives 80% power to detect a 25% relative risk reduction assuming a 30% baseline risk of intubation or death at 30 days (two-sided test; α =0.05). An interim analysis and sample size re-estimation will be done by an unblinded independent biostatistician after primary outcome data are available for 50% of the target recruitment (n= 600). Discussion: This trial will determine whether CCP will reduce intubation or death non-intubated adults with COVID-19. The trial will also provide information on the role of and thresholds for SARS-CoV-2 antibody titers and neutralization assays for donor qualification.Trial registration: Clinicaltrials.gov NCT04348656; registered 16 April 2020; https://clinicaltrials.gov/ct2/show/NCT04348656?term=NCT04348656&draw=2&rank=1
The Micro-Tribological Behavior and Friction Mechanism of the Graphite/Cu Composites and Copper-coated Graphite-graphite/Cu Composites
Authors: Yi Mao-Zhong , Zhu Linying , Zhang Bei , Xie Aolin 
Published: March 2021

Abstract
The ring-block tribological behavior of the graphite/Cu(G/Cu) composites and copper-coated graphite-graphite/Cu(CCG-G/Cu) were studied by observing the friction coefficient,wear rate,microstructure and morphogoly of the composites after friction experiments.SEM and TEM were used to character the micro-morphology and micro-structure of debris,friction surface and friction cross section of the composites.The results show that adding 20wt% copper-coated graphite could reduced the friction coefficient and wear rate of the composites.The micro-morphology and micro-structure show that the copper phase are undergo oxidation and plastic deformation under cyclic stress,results in abundant deformation area in copper-rich zones.Interlaminar shedding and intramolecular tearing occurs in graphite phase,and then laid flat on the friction surface,forming a friction film with higher integrity and reducing the friction coefficient of the composites.The TEM images of the friction cross section show that deformation zone is mainly composed of accumulation zone,drag zone and carbon film.The simulation of the friction process shows that the initial stage is mainly dominated by abrasive wear and adhesive wear.With the progress of the experiment,the exposed copper phase is oxidized and oxidative wear occurs,graphite is shed and transferred to the contact surface.In the later stage of the experiment,a complete friction film with high graphite content is formed on the contact surface,which is mainly dominated by fatigue wear.
Pulmonary Function Changes in the Older adults with and without Metabolic Syndrome
Authors: Rangel Maysa Brandao , Moraes-Ferreira Renilson , Oliveira-Junior Manoel , Santos-Dias Alana , Bachi André , Gabriela-Pereira Giovana , Freitas Simone de Oliveira , Araújo-Rosa Amanda , Oliveira Luis , Frison Claudio , Raju Raghavan , Balagopal P , Vieira Rodolfo 
Published: March 2021

Abstract
Low-grade inflammation associated with metabolic syndrome (MS) triggers alterations in several organs, but its effects on pulmonary functional and immunological response in older adults are unknown. This cross-sectional study investigated such responses in older adults with and without MS. The study consisted of 77 older adults with MS (68 ± 3 years old) and without MS (67 ± 3 years old). Impulse oscillometry (IOS) was used to evaluate airway and tissue resistance and reactance. Biomarkers of systemic and pulmonary inflammation and fibrosis were studied. Total resistance of respiratory system (R5Hz; p < 0.009), and resistance of proximal (R20Hz; p < 0.001) and distal (R5Hz-R20Hz; p < 0.004) airways were impaired in MS individuals compared to those without MS. The levels of pro-inflammatory (leptin; IL-1beta; IL-8, p < 0.001; TNF-alpha, p < 0.04) and pro-fibrotic (VEGF, p < 0.001) factor increased in MS, while reduced levels of anti-inflammatory cytokines (adiponectin; IL-1ra; IL-10; p < 0.001), and anti-fibrotic (relaxin 1; relaxin 3; Klotho, p < 0.001) factors were found. We conclude that MS accelerates lung function and mechanics impairment in older adults in detriment of an imbalance between pro and anti-inflammatory and fibrotic mediators. Furthermore, this study shows that the lungs also are a target organ in MS, deserving clinical assessment in older adults’ population.
Designing a Statistical&nbsp;Procedure for Monitoring Global Carbon Dioxide Emissions
Authors: Bennedsen Mikkel 
Published: March 2021

Abstract
Following the Paris Agreement of 2015, most countries have agreed to reduce their carbon dioxide (CO2) emissions according to individually set Nationally Determined Contributions. However, national CO2 emissions are reported by individual countries and cannot be directly measured or verified by third parties. Inherent weaknesses in the reporting methodology may misrepresent, typically an under-reporting of, the total national emissions. This paper applies the theory of sequential testing to design a statistical monitoring procedure that can be used to detect systematic under-reportings of CO2 emissions. Using simulations, we investigate how the proposed sequential testing procedure can be expected to work in practice. We find that, if emissions are reported faithfully, the test is correctly sized, while, if emissions are under-reported, detection time can be sufficiently fast to help inform the 5 yearly global "stocktake" of the Paris Agreement. We recommend the monitoring procedure be applied going forward as part of a larger portfolio of methods designed to verify future global CO2 emissions.