bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulatory VCAN Polymorphism is Associated with Shoulder Pain and Disability in Breast Cancer Survivors
Authors: Mafu Trevor Shepherd , September Alison V , Shamley Delva 
Published: February 2021

Abstract
Background and PurposeShoulder morbidity following breast cancer treatment is multifactorial. Despite several treatment- and patient-related factors implicated, unexplained inter-individual variability exists in the development of such morbidity. Given the paucity of relavant genetic studies, we investigate the role of polymorphisms in candidate proteoglycan genes.Patients and methodsWe conducted a cross-sectional study on 254 South African breast cancer survivors, to evaluate associations between shoulder pain/disability and ten single nucleotide polymorphisms (SNPs) within four proteoglycan genes: ACAN (rs1126823 G>A, rs1516797 G>T, rs2882676 A>C); BGN (rs1042103 G>A, rs743641 A>T, rs743642 G>T); DCN rs516115 C>T; and VCAN (rs11726 A>G, rs2287926 G>A, rs309559). Participants were grouped into no–low and moderate–high shoulder pain/disability based on total pain/disability scores: <30 and ≥30, respectively using the shoulder pain and disability index (SPADI).ResultsThe GG genotype of VCAN rs11726 was independently associated with an increased risk of being in the moderate to high shoulder pain (P=0.005, OR=2.326, 95% CI=1.259 - 4.348) or disability (P=0.011, OR=2.439, 95% CI=1.235 - 4.762) categories, after adjusting for participants’ age. In addition, the T-T-G inferred allele combination of BGN (rs74364 - rs743642) - VCAN rs11726 was associated with an increased risk of being in the moderate to high shoulder disability category (0=0.002, OR=2.347, 95% CI=1.215 - 4.534).ConclusionOur study is first to report that VCAN rs11726, independently or interacting with BGN polymorphisms, is associated with shoulder pain or disability in breast cancer survivors. Whereas our findings suggest an involvement of proteoglycans in the etiology of shoulder pain/disability, further studies are recommended.
The Predicting Role of the Safety Climate in the Professional Behavior of Nurses: A Cross-Sectional Study
Authors: Bagherzadeh Razieh , Salemipoor Yasmin , Hajinejad Fatemeh , Heidari Fatemeh , Vahedparast Hakimeh 
Published: February 2021

Abstract
Background: Safety climate which is a subset of organizational climate in the field of safety mirrors the attitude of people in care centers towards safety. In addition to being in connection with diverse parts of the organization’s function, safety climate can also affect the nurse’s performance. Aim: The present study aimed at determining the predicting role of the safety climate in the professional behavior of nurses working in hospitals affiliated to Bushehr University of Medical Sciences. Methods: In this cross sectional study, which is of descriptive analytical type, 595 expert nurses currently working with more than 6 months of working experience in the medical wards of hospitals affiliated to Bushehr University of Medical Sciences entered the study. Data was gathered from the viewpoint of the nurse and professional behavior through a demographic information form and two safety climate questionnaires. Then the data were analyzed by V.19 SPSS which is statistical software. The data analysis was conducted using univariate regression and multivariate linear regression at the significance level of 0.05. Results: Among the diverse domains of the safety climate, the field of nursing education (P= 0.027, ß=0.104), communicating with other nurses (P= 0.027, ß=0.101) and error reporting (P= <0.001, ß= 0.191) were the direct prediction of professional nursing behavior. Also, apart from safety climate, satisfaction of the nursing job had a direct, statistic and significant relationship with professional behavior (P= <0.001, ß= 0.142). Conclusion: Attempts to create a ward, in which the nurses receive the necessary education in an appropriate time and ameliorate their expertise, as well as a climate in which nurses have such a good relationship with their collages that they can easily talk about the possible mistakes and errors, can upgrade nurse’s professional behavior.
Identification of cis-regulatory motifs in first introns and the prediction of intron-mediated enhancement of gene expression in the plant Arabidopsis thaliana
Authors: Back Georg , Walther Dirk 
Published: February 2021

Abstract
Background. Intron mediated enhancement (IME) is the potential of introns to enhance expression of its respective gene. This essential function of introns has been observed in a wide range of species, including fungi, plants, and animals. Studies in the plant ​ Arabidopsis thaliana have shown that enhancing introns exhibit a distinct base composition and are generally the first intron located close to the transcription start site. However, the mechanisms underlying the enhancement are as of yet poorly understood. The goal of the study was to identify potential IME-related sequence motifs and genomic features found in first introns of genes in the plant Arabidopsis thaliana. Results. Based on the rationale that functionale sequence motifs are evolutionarily conserved, we exploited the deep sequencing information available for Arabidopsis thaliana, covering more than one thousand Arabidopsis accessions, and identified 81 candidate hexamer motifs with increased conservation across all accessions, and which also exhibited positional occurrence preferences. Of those, 71 were found associated with increased correlation of gene expression of genes harboring them, suggesting a cis-regulatory role. Filtering further for effect on gene expression correlation yielded a set of 16 hexamer motifs, corresponding to five consensus motifs. While all five motifs represent new motif definitions, two are similar to the two previously reported IME-motifs, whereas three are altogether novel. To identify additional IME-related genomic features, Random Forest models were trained for classification of gene expression level based on an array of different sequence-related features. The results indicate that introns harbor information with regard to gene expression level and suggest sequence-compositional features as most informative, while position-related features, that were thought to be of central importance before, were found with lower than expected relevance. Conclusions. ​ Exploiting deep sequencing and broad gene expression information and on a genome-wide scale, this study confirmed the regulatory role on first-introns, characterized their intra-species conservation, and identified a set of novel sequence motifs located in first introns of genes in the genome of the plant ​ Arabidopsis thalian​ a that may play a role in inducing high and correlated gene expression of the genes harboring them.
&nbsp;Time To Death Among HIV-Infected Under-Five Children After Initiation Of Anti-Retroviral Therapy And Its Predictors In Oromiya Liyu Zone, Amhara Region, Ethiopia: A Retrospective Chohort
Authors: Arega Weldemariam Sintayehu , Marie Bereka Tefera , Bazezew Bitewa Yibelu 
Published: February 2021

Abstract
Background: Human Immunodeficiency Virus (HIV) is infection which mainly attacks immune system of an individual. Its disease progress is rapid in children and if treatment is not initiated nearly half of infected children will die by the second year of infection. In Ethiopia, nearly twenty four percent of HIV related death is happen to under-five children; however studies done in this specific age group are limited are with poor evidence of predictors. Objectives: To determine time to death and identify predictors of death in HIV infected under-five children on antiretroviral therapy in Amhara regional state, Oromia ‘liyu’ zone, Northeast Ethiopia, from 2014 to 2019. Methods: Institution based retrospective follow up study was conducted in 376 under-five HIV-infected children on antiretroviral therapy from January 2014 to December 2019 in health institutions in Oromia Liyu Zone, Amhara region, Ethiopia. Multivariable Cox-proportional hazard regression model was used to identify independent predictors of Mortality in HIV-infected under-five children on antiretroviral therapy. Results: At the end of follow up, 285 (80.28%) of HIV-infected children were alive, 39 (10.99%) were lost to follow up, 11 (3.94%) were transferred out and 21 (5.59%) were reported dead due to HIV/AIDS. The cumulative survival probabilities of children after 3, 6, 12, 24 and 36 months were 0.99, 0.98, 0.97, 0.89 and 0.87 respectively. The overall mean time to death was 19.7 months (95%CI= 18.74-20.67) with incidence of 5.9 deaths per 100 child-months (95%CI: 3.89-9.09). Children with severe malnutrition at baseline (AHR=4.9; 95 CI: 1.04, 23.50), advanced WHO clinical stage at enrolment (AHR=3.9; CI: 1.37, 10.88), poor adherence to ART (AHR=6.56; CI: 3.33, 10.14) and with no history of Isoniazide prophylaxis were significantly associated to higher mortality events (AHR=3.6; CI: 1.24, 10.18). Conclusion: Death of HIV-infected under-five children on ART is high within the first one year after enrolment. The risk of death increased if the child was malnourished at begging of treatment, had poor ART adherence, with advanced WHO clinical stages and lack of Isoniazide prophylaxis during their age of infancy.
Geo-Spatially Based Analysis And Economic Feasiblity Evaluation Of Waste-To-Energy Facilities: A Case Study Of Local Government Areas Of Anambra State Of Nigeria
Authors: Chukwuma Emmanuel , Ojediran John , Anizoba Daniel Azikiwe , Ubah Joseph Azikiwe , Nwachukwu Pius 
Published: February 2021

Abstract
Access to affordable clean energy source as stipulated in UN SDG goal number 7 is important for the development and socio-economic well-being of people, the need for proper assessment of resources to achieve this goal is indisputable. The goal of this study is to assess the economic feasibility of utilizing organic fraction of Municipal Solid Wastes (MSWof) using a case study of 21 Local Government Areas (LGA) or Authority for Anambra State of Nigeria for waste to energy project. The quantity of organic fraction of MSW, energy recovery and optimum number of plants at the various LGAs was estimated. The result of the study indicates that about 198 tons maximum value of MSWof can be generated daily in Aguata LGA, with electric energy potential value of 545MW. The number of plants for the LGAs ranged from 10 to 50 and from 3 to 12 for small and medium scale plants. A large scale plant of about 50m3 can possibly be installed at all the LGAs, with a maximum of 4 plants in Idemili and Aguata LGA. The economic assessment based on Net Present Value (NPV) criteria shows poor economic feasibility for small scale plant, while NPV was positive for medium and large scale plants. The Internal Rate of Return (IRR) ranged from 0.32 to 0.94, with a general increase from small scale to large scale economic feasibility. It is suggested that the autonomy of the various LGAs in the country should serve as a major motivation in adopting bio-energy projects independently, and this study will serve as a decision toolkit in the appropriate scale to be adopted.
Phase IB Study of Sorafenib and Evofosfamide in Patients with Advanced Hepatocellular and Renal Cell Carcinomas (NCCTG N1135, Alliance)
Authors: Tran Nguyen , Foster Nathan , Mahipal Amit , Byrne Thomas , Hubbard Joleen , Silva Alvin , Mody Kabir , Alberts Steven , Borad Mitesh 
Published: February 2021

Abstract
Background: Sorafenib (Sor) remains a first-line option for hepatocellular carcinoma (HCC) or refractory renal cell carcinomas (RCC). PLC/PRF/5 HCC model showed upregulation of hypoxia with enhanced efficacy when Sor is combined with hypoxia-activated prodrug evofosfamide (Evo). Methods: This phase IB 3 + 3 design investigated 3 Evo dose levels (240, 340, 480 mg/m2 on days 8, 15, 22), combined with Sor 200 mg orally twice daily (po bid) on days 1-28 of a 28-day cycle. Primary objectives included determining maximum tolerated dose (MTD) and recommended phase II dose (RP2D) of Sor + Evo. Results: Eighteen patients were enrolled (median age 62.5 years; 17 male /1 female; 12 HCC/6 RCC) across three dose levels (DL0: Sor 200 mg bid/Evo 240 mg/m2 [n=6], DL1:Sor 200 mg bid/Evo 480 mg/m2 [n=5], DL1a: Sor 200 mg bid/Evo 340 mg/m2 [n=7]). Two dose-limiting toxicities (DLTs) were reported with Evo 480 mg/m2 (grade 3 mucositis, grade 4 hepatic failure). Grade 3 rash DLT was observed in one patient at Evo 240 mg/m2. No DLTs were observed at Evo 340 mg/m2. MTD and RP2D were established as Sor 200 mg/Evo 340 mg/m2 and Sor 200/Evo 240 mg/m2, respectively. The most common treatment-related adverse events included fatigue, hand-foot syndrome, hypertension, and nausea/vomiting. Two partial responses were observed, one each at DL0 and DL1a.; disease control rate was 55%.Conclusions: RP2D was established as sorafenib 200 mg bid + Evo 240 mg/m2. While preliminary anti-tumor activity was observed, future development must account for advances in immunotherapy in HCC/RCC. NCT01497444Date of registration December 20, 2011
A distance-based paper sensor for rapid detection of blood lactate concentration using gold nanoparticles synthesized by Satureja hortensis
Authors: Mirzaei Younes , Gholami Ali , Bordbar Mohammad Mahdi 
Published: February 2021

Abstract
Rapid detection of lactate as the major fuel source and material to support the level of blood sugar can open up a new class of analysis for nutritionists and sports medicine practitioners. Here, a rapid and user-friendly method for monitoring changes in the blood lactate concentration is reported, involving the use of a paper strip coated with gold nanoparticles (AuNPs). The aqueous extract of Satureja hortensis is used as the reduction agent in a green synthesis of the AuNPs. Due to the interaction between the NPs and lactate flowing across the paper strip, they change color from red to purple, which can be detected by the naked eye. It is possible to create a distance-based paper sensor, representing these changes as a column of colored bars on the paper substrate whose length has a good linear relationship with the lactate concentration ranged between 1.0 and 30.0 mM. By recording the color changes with a scanner, the sensor response is also obtained using an image analysis method, leading to a linear calibration curve in the lactate concentration range of 0.5 to 30.0 mM. The resulting detection limits of the lactate measurements are found to be 0.6 and 0.4 using the distance measurement and image analysis methods, respectively. Interestingly, in the presence of interfering species, the sensor is selective for lactate, while also showing good performance in measuring the lactate concentration in the real blood samples.
Transferability and Characterization of Microsatellite Markers from Byrsonima Cydoniifolia A. Juss. (MALPIGHIACEAE) in Seven Related Taxa from Cerrado Biome Reveal Genetic Relationships
Authors: Bernardes Vanessa , Murakami Devanir M. , Bizão Nair , Souza Tamara N. , Silva Marcos J. da , Telles Mariana P.C. 
Published: February 2021

Abstract
Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.
Eosinophilic Gastroenteritis With Ascites During Pregnancy
Authors: Rhyou Hyo-In , Oh So Ra , Nam Young-Hee 
Published: February 2021

Abstract
Background: Eosinophilic gastroenteritis (EGE), a rare disorder of unknown etiology, is characterized by eosinophilic infiltration of the gastrointestinal tract in the absence of secondary causes for eosinophilia. EGE has nonspecific symptoms, but abdominal pain, nausea, and vomiting are the most common presenting symptoms. Eosinophilic ascites is a very unusual presentation. Case presentation: A 38-year-old women at 11 weeks of gestation presented with nausea, diarrhea, and abdominal pain. Magnetic resonance imaging revealed diffuse bowel wall thickening and a small amount of ascites. Despite antibiotic treatment, her symptoms were persistent and she developed marked abdominal distension and weight gain despite antibiotic treatment. Her blood eosinophil count was high, and ultrasonography showed large ascites. Diagnostic paracentesis revealed significant eosinophilia. She completely recovered with steroid treatment and delivered a healthy baby without any complications.Conclusions: To our knowledge, this is the first case of EGE presenting with ascites during pregnancy. The serosal EGE is very rare type of EGE, but it can occur during pregnancy. The augmented Th2 immunity during pregnancy may be related with EGE.
High Light and High Temperature Reduce Photosynthesis
via Different Mechanisms in the C4 Model Setaria viridis
Authors: Anderson Cheyenne , Mattoon Erin , Zhang Ningning , Becker Eric , McHargue William , Yang Jiani , Patel Dhruv , Dautermann Oliver , McAdam Scott , Tarin Tonantzin , Pathak Sunita , Avenson Tom , Berry Jeffrey , Braud Maxwell , Niyogi Krishna , Wilson Margaret , Nusinow Dmitri , Vargas Rodrigo , Czymmek Kirk , Eveland Andrea , Zhang Ru 
Published: February 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Antimicrobial-Associated Anaphylaxis Consequences on Infection Related Mortality and Prolonged Hospitalization
Authors: Esba Laila Carolina Abu , Sehli Faisal Aqeel 
Published: February 2021

Abstract
Background: Antimicrobial-associated anaphylaxis occurs at different rates and can lead to worsening infection-related outcomes, we sought to describe the incidence and complications of such episodes at a tertiary care hospital.Method: A retrospective cohort study was conducted between January 2016 and December 2019. Cases of antimicrobial-associated anaphylaxis were identified using the hospital’s electronic healthcare records. Outcomes included: mortality related to anaphylaxis, infection-related mortality, prolonged hospitalization and impact on antimicrobial prescribing. Results: The estimated rate of antimicrobial-associated anaphylaxis was 18.6 (95% CI: 11.8 –29.5) cases per 100,000 exposures. Prolonged hospitalization was seen in 52.4% of the cases, and major predictors for prolonged hospitalization were a switch to a more toxic antimicrobial (P 0.029) and switch to a broader spectrum antimicrobial (P 0.033). Conclusion: Implications from antimicrobial-associated anaphylaxis is beyond the episode itself, and can be associated with poor clinical outcomes such as infection-related mortality and prolonged hospitalization.
Identity and Chemical Composition of an Albino Mutant Chanterelle
Authors: Thorn R. Greg , Banwell Alicia , Pham Thu Hong (Nicole) , Vidal Natalia P. , Manful Charles F. , Nadeem Muhammad , Ivanov Alexander G. , Mroz Beth Szyszka , Bonneville Michael B. , Hüner Norman Peter Andrew , Piercey-Normore Michele D. , Thomas Raymond 
Published: February 2021

Abstract
White chanterelles (Basidiomycota), lacking the orange pigments and apricot-like odour of typical chanterelles, were found recently in the Canadian provinces of Québec (QC) and Newfoundland & Labrador (NL). Phylogenetic analyses confirmed all samples of white chanterelles from NL and QC as Cantharellus enelensis; we name these forma acolodorus. We characterized carotenoid pigments, lipids, phenolics, and volatile compounds in these and related chanterelles. White mutants of C. enelensis lacked detectable β-carotene, confirmed to be the primary pigment of wild-type, golden-orange individuals, and could also be distinguished by their profiles of fatty acids and phenolic acids, and by the ketone and terpene composition of their volatiles. We detected single base substitutions in the phytoene desaturase (Al-1) and phytoene synthase (Al-2) genes of the white mutant, which are predicted to result in altered amino acids in their gene products and may be responsible for the loss of β-carotene synthesis in that form.
GRP75-mediated upregulation of HMGA1 stimulates stage I lung adenocarcinoma progression by activating the JNK/c-JUN signaling
Authors: Qiao Guo-Bing , Wang Ren-Tao , Wang Shu-Nan , Tao Shao-Lin , Tan Qun-You , Jin Hua 
Published: February 2021

Abstract
Background

Recurrence is a major challenge in early-stage lung adenocarcinoma (LUAD) treatment. But the mechanism is largely unknown. Here, we investigated the role and mechanism of high-mobility group AT-hook 1 (HMGA1) and glucose-regulated protein 75-kDa (GRP75) in stage I LUAD and evaluated their potential as biomarkers for predicting the recurrence and prognosis of stage I LUAD.

Methods

The Cancer Genome Atlas (TCGA) dataset was used to investigate the clinical significance of HMGA1 and GRP75 in early-stage LUAD. Western blotting and immunohistochemistry were used to measure protein expression levels. The biological functions of HMGA1 and GRP75 in LUAD were investigated both in vitro and in vivo through overexpression and knockdown experiments. The interaction and regulation between HMGA1 and GRP75 were evaluated with coimmunoprecipitation and ubiquitination assays. The downstream signaling pathway of the GRP75/HMGA1 axis was investigated by mRNA-sequencing analysis.

Results

Both HMGA1 expression levels and GRP75 expression levels were associated with recurrence in stage I LUAD patients. In particular, HMGA1 had potential as an independent prognostic factor in stage I LUAD patients. Overexpression of GRP75 or HMGA1 significantly stimulated LUAD cell growth and metastasis, while silencing GRP75 or HMGA1 inhibited LUAD cell growth and metastasis in vitro and in vivo. Importantly, GRP75 inhibited ubiquitination-mediated HMGA1 degradation by directly binding to HMGA1, thereby causes HMGA1 upregulation in LUAD. In addition, the GRP75/HMGA1 axis played its role by activating JNK/c-JUN signaling in LUAD.

Conclusions

The activation of GRP75/HMGA1/JNK/c-JUN signaling is an important mechanism that promotes the progression of stage I LUAD, and a high level of HMGA1 is a novel biomarker for predicting recurrence and a poor prognosis in stage I LUAD patients.