bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATF2-Induced Overexpression of lncRNA LINC00882, as a Novel Therapeutic Target, Accelerates Hepatocellular Carcinoma Progression via Sponging miR-214-3p to Upregulate CENPM
Authors: Ren Hua , Wei Zhi-cheng , Sun Yan-xia , Qiu Chun-yan , Zhang Wen-jue , Zhang Wei , Liu Tao , Che Xu 
Published: September 2021

BackgroundLong intergenic non-protein coding RNA 882 (LINC00882) are abnormally expressed in several tumors. Our research aimed to uncover the functions and the potential mechanisms of LINC00882 in hepatocellular carcinoma (HCC) progression.MethodsRT-qPCR was applied to identify LINC00882 and miR-214-3p levels in HCC specimens and cells. Luciferase reporter was applied for the exploration of whether activating transcription factor 2 (ATF2) could bind to the promoter region of LINC00882. Cell proliferation, invasion, and migration were evaluated. In vivo tumor xenograft models were constructed to assess tumorigenicity. RT-PCR, Western blot and Luciferase reporter assays were conducted to examine the regulatory relationships among LINC00882, miR-214-3p and ATF2.ResultsLINC00882 was markedly upregulated in HCC cells and clinical specimens. Additionally, ATF2 could bind directly to the LINC00882 promoter region and activate its transcription. Loss-of-function studies further demonstrated that LINC00882 knockdown inhibited proliferation, invasion, and migration of HCC cells. Mechanistically, LINC00882 adsorbed miR-214-3p, thus promoting the expressions of CENPM. Rescue assays demonstrated that functions of LINC00882 deficiency in HCC cells were reversed through suppressing miR-214-3p.ConclusionOur group identified a novel regulatory axis of ATF2/LINC00882/miR-214-3p/CENPM, which may provide potential therapeutic targets for HCC.
Ruxolitinib in Patients With Chronic Active Epstein-Barr Virus Infection: A Retrospective, Single-Center Study
Authors: Song Yue , Wang Jingshi , Wang Yini , Wang Zhao 
Published: September 2021

Background: Chronic active Epstein-Barr virus (CAEBV) infection is one of the EBV-positive T- or NK-cell lymphoproliferative diseases. There is no safe and effective treatment currently and the only proven curable therapy is allogeneic hematopoietic stem cell transplantation (allo-HSCT). The JAK1/2 inhibitor, ruxolitinib, is now considered a novel therapy in inflammatory disease, and hypercytokinemia is an important feature of CAEBV.Method: All patients who suffered active CAEBV and were treated with ruxolitinib as compassionate use in our center from Sep 1, 2017, and Apr 30, 2019, were retrospectively analyzed.Results: In general, seven out of nine patients responded to ruxolitinib. Six out of seven patients became afebrile within 48 h. The AST/ALT level of three out of four patients decreased after ruxolitinib treatment. Two patients with cytopenia recovered. No significant decrease in the EBV-DNA copy number was observed (p = 0.161). For those seven patients who responded to ruxolitinib, the median continuing period in remission was 7.1 weeks (range, 3.4–101.0 weeks). Two patients achieved long-term stable remission with ruxolitinib monotherapy. None of these patients discontinued ruxolitinib due to the possible toxicity.Conclusion: Ruxolitinib is an effective and rather safe option for controlling the inflammatory symptoms of active CAEBV, especially in patients with CAEBV who have failed previous treatments or have relapsed. It can also play a promising role in improving the quality of daily life of patients and successfully bridging to allo-HSCT.
Designing Short Peptides to Block the Interaction of SARS-CoV-2 and Human ACE2 for COVID-19 Therapeutics
Authors: Basit Abdul , Karim Asad Mustafa , Asif Muhammad , Ali Tanveer , Lee Jung Hun , Jeon Jeong Ho , Rehman Shafiq ur , Lee Sang Hee 
Published: September 2021

To date, the current COVID-19 pandemic caused by SARS-CoV-2 has infected 99.2 million while killed 2.2 million people throughout the world and is still spreading widely. The unavailability of potential therapeutics against this virus urges to search and develop new drugs. SARS-CoV-2 enters human cells by interacting with human angiotensin-converting enzyme 2 (ACE2) receptor expressed on human cell surface through utilizing receptor-binding domain (RBD) of its spike glycoprotein. The RBD is highly conserved and is also a potential target for blocking its interaction with human cell surface receptor. We designed short peptides on the basis of our previously reported truncated ACE2 (tACE2) for increasing the binding affinity as well as the binding interaction network with RBD. These peptides can selectively bind to RBD with much higher affinities than the cell surface receptor. Thus, these can block all the binding residues required for binding to cell surface receptor. We used selected amino acid regions (21–40 and 65–75) of ACE2 as scaffold for the de novo peptide design. Our designed peptide Pep1 showed interactions with RBD covering almost all of its binding residues with significantly higher binding affinity (−13.2 kcal mol−1) than the cell surface receptor. The molecular dynamics (MD) simulation results showed that designed peptides form a stabilized complex with RBD. We suggest that blocking the RBD through de novo designed peptides can serve as a potential candidate for COVID-19 treatment after further clinical investigations.
Cardiovascular Indicators of Systemic Circulation and Acute Mountain Sickness: An Observational Cohort Study
Authors: Chen Renzheng , Sun Mengjia , Yang Jie , Liu Chuan , Zhang Jihang , Ke Jingbin , Deng Yuhan , He Chunyan , Yang Yuanqi , Cheng Ran , Yuan Fangzhengyuan , Tan Hu , Gao Xubin , Huang Lan 
Published: September 2021

Background: Acute high-altitude (HA) exposure results in blood pressure (BP) and cardiac function variations in most subjects, some of whom suffer from acute mountain sickness (AMS). Several previous studies have found that cardiovascular function indicators are potentially correlated with AMS.Objectives: This study aims to examine HA-induced cardiovascular adaptations in AMS patients and compare them with healthy subjects. It also aims to investigate the relationship between cardiovascular function indicators and AMS, as well as to provide some insightful information about the prevention and treatment of AMS.Methods: Seventy-two subjects were enrolled in this cohort study. All the subjects ascended Litang (4,100 m above sea level). They were monitored by a 24-h ambulatory blood pressure (ABP) device and underwent echocardiography examination within 24 h of altitude exposure. The 2018 Lake Louise questionnaire was used to evaluate AMS.Results: Acute mountain sickness group consisted of more women (17 [60.7%] vs. 10 [22.7%], p = 0.001) and fewer smokers (5 [17.9%] vs. 23 [52.3%], p = 0.003). Compared with subjects without AMS, subjects with AMS had lower pulse pressure (PP) (daytime PP, 45.23 ± 7.88 vs. 52.14 ± 4.75, p &lt; 0.001; nighttime PP, 42.81 ± 5.92 vs. 49.39 ± 7.67, p &lt; 0.001) and lower effective arterial elastance (Ea) (1.53 ± 0.24 vs. 1.73 ± 0.39, p = 0.023). Multivariate regression indicated that female sex (OR = 0.23, p = 0.024), lower daytime PP (OR = 0.86, p = 0.004), and lower Ea (OR = 0.03, p = 0.015) at low altitude (LA) were independent risk factors for AMS. Combined daytime PP and Ea at LA had a high predictive value for AMS (AUC = 0.873; 95% CI: 0.789–0.956). Correlation analysis showed that AMS-induced headache correlated with daytime PP (R = −0.401, p &lt; 0.001) and nighttime PP at LA (R = −0.401, p &lt; 0.001).Conclusion: Our study demonstrated that AMS patients had a lower PP and Ea at LA. These baseline indicators of vasodilation at LA were closely associated with AMS, which may explain the higher headache severity in subjects with higher PP at LA.
Efficacy of Intrauterine Perfusion of Cyclosporin A for Intractable Recurrent Spontaneous Abortion Patients With Endometrial Alloimmune Disorders: A Randomized Controlled Trial
Authors: Zhao Long , Qi Lijuan , Fu Jinhua , Bi Shuqin , Li Lin , Fu Yinghui 
Published: September 2021

ObjectiveTo explore the therapeutic efficacy of intrauterine perfusion of cyclosporin A (CsA) in intractable recurrent spontaneous abortion (RSA) patients with endometrial alloimmune dysfunction.MethodsThis is a randomized controlled trial (RCT) of patients with intractable RSA with endometrial alloimmune disorders. A total of 201 women were enrolled, all of whom had at least 3 serial abortions and endometrial alloimmune dysfunction. Participants were randomly assigned to two groups. The CsA group (n= 101) received intrauterine infusion of 250 mg CsA on the 3rd and 7th days after menstruation for 2 menstrual cycles, while the placebo group (n= 100) received placebo. The birth of healthy, deformity-free babies was the main study outcome.ResultsIn total, 75 (74.26%) women in the CsA group and 59 (59.00%) women in the placebo group gave birth to healthy babies [P= 0.01,OR= 2.01; 95%CI(1.10∼3.65)]. Compared to the placebo group, the CsA group had dramatically lower endometrial CD56+cell and CD57+cell concentrations at the luteal phase of the second menstrual cycle (P&lt; 0.05).ConclusionIntrauterine perfusion of CsA was confirmed to be a promising approach for the treatment of intractable alloimmune RSA.
A Randomized Waitlist-Controlled Trial of an Intergenerational Arts and Heritage-Based Intervention in Singapore: Project ARTISAN
Authors: Ho Andy Hau Yan , Ma Stephanie Hilary Xinyi , Tan Michael Koon Boon , Bajpai Ram Chandra 
Published: September 2021

Loneliness has become a global major public health concern, with detrimental effects to the young and old. ARTISAN (Aspiration and Resilience Through Intergenerational Storytelling and Art-based Narratives) is a 5-week, 15-h participatory art and group-based intervention that focuses on resilience building and loneliness alleviation among the young and old through a structured multimodal framework held at a museum space. Developed with a Participatory Action Research (PAR) approach, this intervention is evaluated using an open-label waitlist randomized controlled trial design (RCT) comprised of community-dwelling youth and older adults randomized into an intervention group (n = 35) or a waitlist-control group (n = 33). Participants were assessed on standardized self-reported psychometric measures including loneliness, resilience, quality of life, social support, life satisfaction and national identity at three time points. Qualitative data generated during each intervention session as well as acceptability focus groups were recorded and transcribed. Linear mixed modeling analyses revealed that participants in the intervention group experienced improvements in life satisfaction compared to participants in the waitlist-control group (95% CI: 0.22 to 0.77, p &lt; 0.001, Cohen's d = 0.53) immediately after the completion of ARTISAN. Subgroup analyses for youth participants indicated improvements in quality of life (95% CI: 0.16 to 0.52, p &lt; 0.001, d = 1.31) and national identity (95% CI: 0.18 to 0.80, p = 0.002, d = 0.43) in comparison to the waitlist-control group. At 5-weeks follow-up, the intervention group participants continued to experience high levels of life satisfaction (95% CI: 0.04 to 0.42, p = 0.017, d = 0.47), enhancements in resilience (95% CI: 0.07 to 0.55, p = 0.011, d = 0.46), as well as a significant reduction in loneliness (95% CI: −0.34 to −0.08, p = 0.001, d = 0.61) compared to baseline, reflecting the effectiveness and positive residual effects of the ARTISAN intervention. Similarly, the qualitative findings provided support for the intervention and additional insights to the quantitative findings. This holistic intervention framework that integrates stories, arts and heritage for bridging and empowering lives fills a critical gap in knowledge and practice between the arts, health and citizenship, paving the way for further research in creating a more caring and inclusive society with the arts.Clinical Trials Registration:www.ClinicalTrials.gov, identifier: NCT03048708.
Superlow Friction of a-C:H Coatings in Vacuum: Passivation Regimes and Structural Characterization of the Sliding Interfaces
Authors: Kuwahara Takuya , Long Yun , De Barros Bouchet Maria-Isabel , Martin Jean Michel , Moras Gianpietro , Moseler Michael 
Published: September 2021

A combination of atomistic simulations and vacuum tribometry allows atomic-scale insights into the chemical structure of superlubricious hydrogenated diamond-like carbon (a-C:H) interfaces in vacuum. Quantum molecular dynamics shearing simulations provide a structure-property map of the friction regimes that characterize the dry sliding of a-C:H. Shear stresses and structural properties at the sliding interfaces are crucially determined by the hydrogen content CH in the shear zone of the a-C:H coating. Extremely small CH (below 3 at.%) cause cold welding, mechanical mixing and high friction. At intermediate CH (ranging approximately from 3 to 20 at.%), cold welding in combination with mechanical mixing remains the dominant sliding mode, but some a-C:H samples undergo aromatization, resulting in a superlubricious sliding interface. A further increase in CH (above 20 at.%) prevents cold welding completely and changes the superlubricity mechanism from aromatic to hydrogen passivation. The hydrogen-passivated surfaces are composed of short hydrocarbon chains hinting at a tribo-induced oligomerization reaction. In the absence of cold welding, friction strongly correlates with nanoscale roughness, measured by the overlap of colliding protrusions at the sliding interface. Finally, the atomistic friction map is related to reciprocating friction experiments in ultrahigh vacuum. Accompanying X-ray photoelectron and Auger electron spectroscopy (XPS, XAES) analyses elucidate structural changes during vacuum sliding of a hydrogen-rich a-C:H with 36 at.% hydrogen. Initially, the a-C:H is covered by a nanometer-thick hydrogen-depleted surface layer. After a short running-in phase that results in hydrogen accumulation, superlubricity is established. XPS and XAES indicate a non-aromatic 1–2-nm-thick surface layer with polyethylene-like composition in agreement with our simulations.
PENERAPAN COST REDUCTION STRATEGIES DI MASA PANDEMI COVID-19 PADA USAHA KECIL MENENGAH (UMKM) KOTA BOGOR (Studi pada Anggota UMKM di Komunitas Kefir Bogor)
Authors: Hastuti Sri 
Published: September 2021

<p><em>This research purpose to know the application of Cost Reduction Strategies on Micro Small and Medium Enterprises (MSME).</em> <em>Cost reduction strategies have the meaning of "change" even though it is better known as "cost reduction" but any company that dares to implement cost reduction means that it is ready to change. The problem in the application of Cost Reduction Strategies is the difficulty in making a priority scale to determine cost items that can be streamlined and not optimal in carrying out the cost reduction stage. The focus of this study is more on the application of business process change strategies and also the efficiency of human resources. This research uses a qualitative description method. The technique of collecting data was done by interviewing and surveying a number of respondents using a sampling technique. The survey results showed 67% applied the concept of efficiency in business processes and 25% implemented a human resource efficiency strategy (streamlining), 8% of respondents stated that they did not implement an efficiency strategy during a pandemic because their income has actually increased. From the survey results, it shows that the main focus of MSME entrepreneurs when there is a decline in turnover is to make improvements to the production process firsts, streamlining is the final choice.</em></p><p><strong>Key words:</strong> <em>Cost Reduction Strategies, UMKM Bogor, Covid-19</em></p>
PELAPORAN KEUANGAN UMKM BERDASARKAN SAK EMKM (Studi Kasus pada UMKM di PD Pasar Jaya Kramat Jati)
Authors: Setyaningsih Tina , Farina Khoirina 
Published: September 2021

<p class="Style4">The purpose of this research is to statistically examine the factors that influence the preparation of MSMEs financial reporting based on SAK EMKM at PD Pasar Jaya Kramat Jati. The data in this study are primary data, obtained through the distribution of questionnaires totaling 85. Due to special consideration in accordance with predetermined criteria, Therefore the sampling technique uses purposive sampling in this study. Processing in the study uses SmartPLS 3.3.2 software. The result of this study shows that there is evidence that the variables of educational background and business scale have a positive and significant impact on MSMEs financial reporting based on SAK EMKM. While other variables in this study namely accounting understanding and do not have significant influence on MSMEs financial reporting based on SAK EMKM.</p><p><strong><em>Key words:</em></strong><em> SAK EMKM, Financial reporting, UMKM</em></p>
Ciudad Educadora he ahí el problema
Authors: Castro Villarraga Jorge Orlando 
Published: September 2021

Este artículo da cuenta de los propósitos y de algunos aportes conceptuales generados en el Seminario de coordinadores de laCátedra de Pedagogía 2005 en torno a la idea y al modelo de Ciudad Educadora. Recoge las discusiones generadas a través de los protocolos y en las ponencias presentadas en la modalidad de simposio por sus integrantes, inscritas según tres directrices temáticas.
Reforming Local Government Administration: A Case from Jordan
Authors: Tbaishat Rami 
Published: September 2021

This study aims to analyze the attitude and vision of a random sample (400) of heads and individuals of local unit's staff, Mayors and employees of municipalities, leaders of provincial departments to the degree of difficulties confronting Local Administration Units (LAUs) in Jordan to identify the significant effective reform techniques to reform such problems. In this study, qualitative approaches were adopted. A total of thirty individuals were purposively sampled for in-depth and face-to-face consultations, with particular emphasis on identifying the major effective strategies of reform. The study outcome established that the local administration in Jordan is confronted, to a severe extent, with the accompanying difficulties: capacity building, budgetary problems, environmental protection responsiveness, and public participation. The study established that the absence of monetary resources and the lack of local units' sovereignty in managing resident funding is another major challenge. The last challenge was accounted for capacity building. The improvement of an environmental control framework to reduce pollution, creating an effective mechanism to bridge the relationship between municipalities and citizens, the abandonment of central pre-checking frameworks, expanded government budgetary support, and the strengthening of training and development of employees are the absolute most significant reform actions need to be addressed to solve the problems confronting LAUs.

Received: 3 June 2021 / Accepted: 26 July 2021 / Published: 5 September 2021
LIFE QUALITY OF POPULATION OF THE REGION AND PREVENTION OF NON-ADULT FATALITIES AND INJURIES IN FIRES
Authors: Искалин Виктор Иосифович , Домрачев Константин Васильевич , Скибневская Тамара Гагиковна , Кондратьева Любовь Геннадьевна 
Published: September 2021

В статье рассмотрено предложение по использованию функции желательности Харрингтона для нормализации показателей, отражающих состояние регионов в контексте связи случаев гибели и травматизма несовершеннолетних на пожарах с качеством жизни населения регионов и профилактической работы территориальных органов МЧС России. Показана перспективность такого подхода.
The article considers the proposal to use the Harrington desirability function to normalize indicators reflecting the regions condition in the context of connection between fatalities/injuries of non-adults in fires and life quality of population of the regions and the preventive activities of EMERCOM of Russia territorial authorities. The prospects of such approach are shown.
Exploring the 100 au Scale Structure of the Protobinary System NGC 2264 CMM3 with ALMA
Authors: Shibayama Yoshiki , Watanabe Yoshimasa , Oya Yoko , Sakai Nami , López-Sepulcre Ana , Liu Sheng-Yuan , Su Yu-Nung , Zhang Yichen , Sakai Takeshi , Hirota Tomoya , Yamamoto Satoshi 
Published: September 2021

Abstract
We have observed the young protostellar system NGC 2264 CMM3 in the 1.3 mm and 2.0 mm bands at a resolution of about 0.″1 (70 au) with ALMA. The structures of two distinct components, CMM3A and CMM3B, are resolved in the continuum images of both bands. CMM3A has an elliptical structure extending along the direction almost perpendicular to the known outflow, while CMM3B reveals a round shape. We have fitted two 2D Gaussian components to the elliptical structure of CMM3A and CMM3B and have separated the disk and envelope components for each source. The spectral index α between 2.0 and 0.8 mm is derived to be 2.4–2.7 and 2.4–2.6 for CMM3A and CMM3B, respectively, indicating optically thick dust emission and/or grain growth. A velocity gradient in the disk/envelope direction is detected for CMM3A in the CH3CN, CH3OH, and 13CH3OH lines detected in the 1.3 mm band, which can be interpreted as the rotation of the disk/envelope system. From this result, the protostellar mass of CMM3A is roughly evaluated to be 0.1–0.5 M
⊙ by assuming Keplerian rotation. The mass accretion rate is thus estimated to be 5 × 10−5 − 4 × 10−3
M
⊙ yr−1, which is higher than the typical mass accretion rate of low-mass protostars. The OCS emission line shows a velocity gradient in both outflow direction and disk/envelope direction. A hint of outflow rotation is found, and the specific angular momentum of the outflow is estimated to be comparable to that of the disk. These results provide us with novel information on the initial stage of a binary/multiple system.
O “legado” monetarismo de milton Friedman Milton friedman’s “legacy”
Authors: Nascimento Pedro Henrique 
Published: September 2021

O presente estudo se propõe a discutir o legado teórico deixado por um dos maiores economistas do século XX, Milton Friedman, autor esse que revolucionou a teoria econômica ao questionar as proposições keynesianas e propor a moeda como a variável relevante a ser analisada e prescrições políticas que assegurassem a estabilidade econômica por meio de regras sobre esse agregado monetário. O roteiro da avaliação empreendida nesse trabalho foi, primeiramente, uma avaliação empírica da experiência monetarista implementada nos governos Margaret Thatcher (Inglaterra) e Paul Volcker (EUA) e, na sequência, uma discussão teórica sobre o que foi absorvido ou não pelas teorias econômicas que sucederam o monetarismo de Friedman. Com base nessas discussões, o estudo sustenta que a “herança” deixada pelo autor foi pequena quando comparada ao que não foi absorvido pelas demais teorias e a principal razão para esse fato repousa na baixa aderência verificada pela teoria de Friedman e os resultados empíricos observados durante sua experiência nos EUA e na Inglaterra.

This study aims to discuss the theoretical legacy left by one of the greatest economists of the 20th century, Milton Friedman, who revolutionized economic theory by questioning Keynesian propositions and proposing money as the relevant variable to be analyzed and political prescriptions that ensure economic stability through rules on this monetary aggregate. The evaluation tour undertaken in this work was, firstly, an empirical evaluation of the monetarist experience implemented in the Margaret Thatcher (England) and Paul Volcker (USA) governments, and then a theoretical discussion on what was or was not absorbed by the economic theories that succeeded Friedman's monetarism. Based on these discussions, the study argues that the legacy left by the author was small when compared to what was not absorbed by other theories and the main reason for this fact lies in the low adherence verified by Friedman's theory and the empirical results observed during his experiment. in the USA and England.