bluuebunny/crossref_metadata_2025_split huggingface dataset:
An expert consensus statement on biomarkers of ageing for use in intervention studies
Authors: Perri Giorgia , French Chloe , Agostinis-Sobrinho César , Anand Atul , Antarianto Radiana Dhewayani , Arai Yasumichi , Baur Joseph A , Cauli Omar , Clivaz-Duc Morgane , Colloca Giuseppe , Demetriades Constantinos , de Lucia Chiara , Di Gessa Giorgio , Diniz Breno S , Dotchin Catherine L , Eaglestone Gillian , Elliott Bradley T , Espeland Mark A , Ferrucci Luigi , Fisher James , Grammatopoulos Dimitris K , Hardiany Novi S , Hassan-Smith Zaki , Hastings Waylon J , Jain Swati , Joshi Peter K , Katsila Theodora , Kemp Graham J , Khaiyat Omid A , Lamming Dudley W , Gallegos Jose Lara , Madeo Frank , Maier Andrea B , Martin-Ruiz Carmen , Martins Ian J , Mathers John C , Mattin Lewis R , Merchant Reshma A , Moskalev Alexey , Neytchev Ognian , Ni Lochlainn Mary , Owen Claire M , Phillips Stuart M , Pratt Jedd , Prokopidis Konstantinos , Rattray Nicholas J W , Rúa-Alonso María , Schomburg Lutz , Scott David , Shyam Sangeetha , Sillanpää Elina , Tan Michelle M C , Teh Ruth , Tobin Stephanie W , Vila-Chã Carolina J , Vorluni Luigi , Weber Daniela , Welch Ailsa , Wilson Daisy , Wilson Thomas , Zhao Tongbiao , Philippou Elena , Korolchuk Viktor I , Shannon Oliver M 
Published: December 2024

Abstract
Biomarkers of ageing serve as important outcome measures in longevity-promoting interventions. However, there is limited consensus on which specific biomarkers are most appropriate for human intervention studies. This work aimed to address this need by establishing an expert consensus on biomarkers of ageing for use in intervention studies via the Delphi method.
A three-round Delphi study was conducted using an online platform. In Round 1, expert panel members provided suggestions for candidate biomarkers of ageing. In Rounds 2 and 3, they voted on 500 initial statements (yes/no) relating to 20 biomarkers of ageing. Panel members could abstain from voting on biomarkers outside their expertise. Consensus was reached when there was ≥70% agreement on a statement/biomarker.
Of the 460 international panel members invited to participate, 116 completed Round 1, 87 completed Round 2, and 60 completed Round 3. Across the 3 rounds, 14 biomarkers met consensus that spanned physiological (e.g., insulin-like growth factor 1, growth-differentiating factor-15), inflammatory (e.g., high sensitivity c-reactive protein, interleukin-6), functional (e.g., muscle mass, muscle strength, hand grip strength, Timed-Up-and-Go, gait speed, standing balance test, frailty index, cognitive health, blood pressure), and epigenetic (e.g., DNA methylation/epigenetic clocks) domains.
Expert consensus identified 14 potential biomarkers of ageing which may be used as outcome measures in intervention studies. Future ageing research should identify which combination of these biomarkers has the greatest utility.
Neuroprotective Effect of 1α,25-Dihydroxyvitamin D3 Against Cognitive Impairment in d-Galactose-Induced Aging Mice
Authors: Cai Ming , Wang Yiting , Lu Jingjing , Liang Yongchao , Yi Wenjie , Jiang Fei 
Published: December 2024

Abstract
Aging and age-related cognitive impairment have emerged as a growing global public health concern, yet there are no effective preventive strategies. Excessive oxidative stress and neuroinflammation have been proven to contribute to cognitive decline. Vitamin D maintains the redox balance and exerts immunomodulatory effects, but the specific role of vitamin D in aging and age-related cognitive impairment remains elusive. This study explored the neuroprotective effects and the potential molecular mechanisms of 1α,25-dihydroxyvitamin D3 in the aging model. An aging model was established by the treatment of d-galactose for 14 weeks in male KM mice. 0.1, 0.5, or 1 μg/kg 1α,25-dihydroxyvitamin D3 were used in the intervention group for 8 weeks. Cognitive performance was evaluated using the Morris water maze test, and the levels of oxidative stress and neuroinflammation in the hippocampus were further analyzed. d-galactose induced memory impairment, whereas 1α,25-dihydroxyvitamin D3 intervention prevented cognitive decline, accompanied by a reduction in neuronal apoptosis, an enhancement of synaptic plasticity, and a decrease in Aβ deposition. Meanwhile, 1α,25-dihydroxyvitamin D3 dramatically attenuated oxidative stress, mitigated microglial cell activation, and ameliorated neuroinflammation by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (AREs) axis and inhibiting the NF-κB signaling pathway. This study provides evidence that 1α,25-dihydroxyvitamin D3 might be a promising nutritional strategy for preventing cognitive decline in aging, thereby facilitating the clinical application and expanding the insight of vitamin D.
Key Predictors of Generativity in Adulthood: A Machine Learning Analysis
Authors: Joshanloo Mohsen 
Published: December 2024

Abstract

Objectives
This study aimed to explore a broad range of predictors of generativity in older adults. The study encompassed over 60 predictors across multiple domains, including personality, daily functioning, socioeconomic factors, health status, and mental well-being.

Methods
This study employed a machine learning algorithm known as Random Forest. Data from the Midlife in the United States survey was used.

Results
Results revealed that social potency, openness, social integration, personal growth, and achievement orientation were the strongest predictors of generativity. Notably, many demographic (e.g., income) and health-related variables (e.g., chronic health conditions) were found to be much less predictive.

Discussion
This study provides new data-driven insights into the nature of generativity. The findings suggest that generativity is more closely associated with eudaimonic and plasticity-related variables (e.g., personal growth and social potency) rather than hedonic and homeostasis-oriented ones (e.g., life satisfaction and emotional stability). This indicates that generativity is an inherently dynamic construct, driven by a desire for exploration, social contribution, and personal growth.
Characterizing broad-band seismic dispersion and attenuation in carbonates with fractures and cavities: a numerical approach
Authors: Wang Yirong , Zhao Luanxiao , Quintal Beatriz , Geng Jianhua 
Published: December 2024

SUMMARY
Carbonates are highly heterogeneous and commonly develop large-scale fracture-cavity systems due to the strong diagenesis effect of dissolution, faulting and karstification. Understanding the seismic wave velocity and attenuation signatures is crucial for hydrocarbon exploration and recovery, carbon capture, geothermal exploitation and groundwater management using seismic methods. Nevertheless, due to the sample size limitation, traditional core-scale experiments and sonic logs fail to effectively unravel the essential factors controlling elastic and anelastic responses of those carbonates with large-scale heterogeneity. We developed a 2-D geology-constrained method for constructing digital rock models of carbonates with fracture-cavity systems, integrating outcrop data and statistical analyses. Using finite element simulations based on Biot's quasi-static equations of poroelastic consolidation, we examine the effects of cavity shape, size and number, as well as fracture number, length, width and angle, on wave dispersion and attenuation characteristics. Deep carbonate rocks with fracture-cavity systems exhibit wideband attenuation spanning over three orders of magnitude (from &lt; 0.01 to &gt;10 Hz), driven by coupled pore pressure relaxation mechanisms in the heterogeneous system. Horizontal attenuation was found to be roughly five times greater than vertical attenuation (as fractures are predominantly in a semi-vertical direction), highlighting significant attenuation anisotropy. While cavities have minimal effects on attenuation, fracture density and geometry—especially longer and narrower fractures—significantly influence wave dispersion and attenuation behaviour. These results underscore the importance of integrating both seismic velocity and attenuation data to enhance reservoir characterization reliability, and highlight the potential of using comprehensive broad-band seismic data and large-offset seismic data to improve geophysical interpretations of complex deep carbonate reservoirs.
Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose
Authors: Paredes Franco Jose Carlos , Güther Maria Lucia Sampaio , Ferguson Michael A J 
Published: December 2024

Abstract
For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.
Expression variation of long noncoding RNAs in dopaminergic cells-derived from stem cells and their MPP+ induced PD models
Authors: Behrouzi Abady Pamsary Setareh , Esmaeili Fariba , Dehghanian Fariba , Bahadori Mohammad Hadi 
Published: December 2024

Abstract
Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder characterized by the progressive loss of nigrostriatal dopaminergic neurons (DA) which can be caused by environmental and genetic factors. lncRNAs have emerged as an important regulatory layer in neurodegenerative disorders, including PD. In this study, we investigated and validated lncRNAs that may serve as diagnostic or therapeutic targets for PD. Key genes associated with midbrain and DA cells were screened by differential gene expression analysis on GSE213100 dataset and candidate lncRNAs were selected for further examination. P19 cells were differentiated into DA cells and received treatment with MPP+ to induce PD-like cytotoxic events, which were confirmed by light microscopy, RT-qPCR, immunofluorescence and flow cytometry. Then, the cells were used to investigate the changes of lncRNAs Malat1, Norad, Snhg1 and Meg3. Here we found that the neuronal phenotype was mainly observed on the 12th day of differentiation and the number of DA markers significantly decreased in PD model cells compared with the control group. Moreover, the expression levels of Meg3, Norad, and Snhg1 were decreased by MPP+ whereas Malat1 level was noticeably higher in MPP+ cells compared to DA cells and the control group. In conclusion, the expression level of lncRNAs was able to show a significant difference between differentiated dopaminergic cells and their Parkinsonian model, thereby improving our understanding of the molecular pathogenesis of PD.
Escherichia coli Nissle Improves Short-Chain Fatty Acid Absorption and Barrier Function in a Mouse Model for Chronic Inflammatory Diarrhea
Authors: Ye Zhenghao , Tan Qinghai , Woltemate Sabrina , Tan Xinjie , Römermann Dorothee , Grassl Guntram A , Vital Marius , Seidler Ursula , Kini Archana 
Published: December 2024

Abstract

Background
Defects in SLC26A3, the major colonic Cl−/HCO3− exchanger, result in chloride-rich diarrhea, a reduction in short-chain fatty acid (SCFA)-producing bacteria, and a high incidence of inflammatory bowel disease in humans and in mice. Slc26a3−/− mice are, therefore, an interesting animal model for spontaneous but mild colonic inflammation and for testing strategies to reverse or prevent the inflammation. This study investigates the effect of Escherichia coli Nissle (EcN) application on the microbiome, SCFA production, barrier integrity, and mucosal inflammation in slc26a3−/− mice.

Methods
In vivo fluid absorption and bicarbonate secretion were assessed in the gut of slc26a3+/+ and slc26a3−/− mice before and during luminal perfusion with 100 mM sodium acetate. Age-matched slc26a3+/+ and slc26a3−/− mice were intragastrically gavaged twice daily with 2 × 108 CFU/100 µL of EcN for 21 days. Body weight and stool water content were assessed daily, and stool and tissues were collected for further analysis.

Results
Addition of sodium acetate to the lumen of the proximal colon significantly increased fluid absorption and luminal alkalinization in the slc26a3−/− mice. Gavage with EcN resulted in a significant increase in SCFA levels and the expression of SCFA transporters in the slc26a3−/− cecum, the predominant habitat of EcN in mice. This was accompanied by an increase in mucus-producing goblet cells and a decrease in the expression of inflammatory markers as well as host defense antimicrobial peptides. EcN did not improve the overall diversity of the luminal microbiome but resulted in a significant increase in SCFA producers Lachnospiraceae and Ruminococcaceae in the slc26a3−/− feces.

Conclusions
These findings suggest that EcN is able to proliferate in the inflamed cecum, resulting in increased microbial SCFA production, decreased inflammation, and improved gut barrier properties. In sufficient dosage, probiotics may thus be an effective anti-inflammatory strategy in the diseased gut.
Validity and reliability of a method to estimate the potential harm of medication errors by considering both the likelihood and degree of harm
Authors: Al Atassi Rached , Chan Wing Shun , Jurjonaite Zivile , Kahsay Rahel , Samson Emily , Jones Matthew D 
Published: December 2024

Abstract

Objectives
To develop a tool to ‘risk score’ the potential harm of a medication error by estimating the probability of a range of potential consequences, and assess its feasibility, validity, and reliability.

Methods
The risk score tool described five levels of potential harm developed from an existing risk matrix. Judges estimated the likelihood of harm matching each level, from which a risk score (0–10) was calculated. Thirty judges (doctors, nurses, and pharmacists) used this risk score and the existing Dean and Barber scale to estimate the potential harm of 50 medication errors, 15 with a known outcome. Two weeks later, the judges re-scored ten of the errors. Reliability was investigated using generalizability theory.

Key findings
Fifty medication errors were judged in a mean of 49 minutes with ≤0.7% missing data. There were no significant differences in the judges’ rating of the realism and ease of use of the two tools. Fifty-nine per cent of judges preferred the risk score tool. For both tools, there was a clear relationship between mean score and known outcomes, with no overlap between outcome categories, confirming discriminative validity. The correlation between scores from both tools (R2 = 0.99) confirmed the concurrent criterion validity of the risk score. For both tools, at least three judges would need to score an error to obtain a generalizability coefficient of ≥0.8 using the mean score as an indicator of potential harm.

Conclusions
The risk score was feasible, valid, and reliable. Its performance was comparable with, but did not exceed, the Dean and Barber scale.
Coevolution of marine phytoplankton and Alteromonas bacteria in response to pCO2 and coculture
Authors: Lu Zhiying , Entwistle Elizabeth , Kuhl Matthew D , Durrant Alexander R , Barreto Filho Marcelo Malisano , Goswami Anuradha , Morris J Jeffrey 
Published: December 2024

Abstract
As a result of human activity, Earth’s atmosphere and climate are changing at an unprecedented pace. Models based on short-term experiments predict major changes will occur in marine phytoplankton communities in the future ocean, but rarely consider how evolution or interactions with other microbes may influence these changes. Here, we experimentally evolved several phytoplankton in coculture with a heterotrophic bacterium, Alteromonas sp. EZ55, under either present-day or predicted future pCO2 conditions. Growth rates of phytoplankton generally increased over time under both conditions, but only Thalassiosira oceanica had evidence of a growth rate tradeoff in the ancestral environment after evolution at elevated pCO2. The growth defects observed in ancestral Prochlorococcus cultures at elevated pCO2 and in axenic culture were diminished after evolution, possibly due to regulatory mutations in antioxidant genes. Except for Prochlorococcus, mutational profiles suggested phytoplankton experienced primarily purifying selection, but most Alteromonas lineages showed evidence of directional selection, where evolution appeared to favor a metabolic switch between growth on small organic acids with cyanobacteria versus catabolism of more complex carbon substrates with eukaryotic phytoplankton. Evolved Alteromonas were also poorer “helpers” for Prochlorococcus, consistent with that interaction being a competitive Black Queen process rather than a true mutualism. This work provides new insights on how phytoplankton will respond to increased pCO2 and on the evolutionary mechanisms governing phytoplankton:bacteria interactions. It also clearly demonstrates that both evolution and interspecies interactions must be considered to predict future marine biogeochemistry.
Biochemical and molecular mechanisms of Rhodococcus rhodochrous IITR131 for polyethylene terephthalate degradation
Authors: Srivastava Pallavi , Subramanian Srikrishna , Manickam Natesan 
Published: December 2024

Abstract

Aims
To isolate polyethylene terephthalate (PET)-degrading bacteria and elucidate the underlying mechanisms of PET biodegradation through biochemical and genome analysis.

Methods and results
Rhodococcus rhodochrous IITR131 was found to degrade PET. Strain IITR131 genome revealed metabolic versatility of the bacterium and had the ability to form biofilm on PET sheet, resulting in the cracks, abrasions, and degradation. IITR131 showed a reduction of 19.7%, exhibiting a half-life of 189.9 d of 0.1 mm PET film in 60 d and formed metabolites bis(2-hydroxyethyl) terephthalate (BHET), terephthalic acid (TPA), and benzoic acid (BA). The draft genome of 5.9 Mb of IITR131 revealed that this bacterium has plethora of genes such as terephthalate 1, 2 dioxygenase, carboxylesterase that together constituted a complete pathway for PET degradation. Moreover, strain IITR131 was found to have a variety of genes encoding for enzymes for the metabolism of several plastic polymers, xenobiotics including chloroalkanes, and polycyclic aromatic hydrocarbons.

Conclusions
Rhodococcus rhodochrous IITR131 demonstrated a significant potential in the biodegradation of PET. The comprehensive genomic and metabolic analyses further elucidated the molecular pathway involved in PET degradation, enhancing our understanding of the mechanisms underlying microbial PET biodegradation. These findings underscore the applicability of R. rhodochrous IITR131 in biotechnological approaches for mitigating plastic pollution.
Impact hazard analysis of islanding face mining based on micro-seismic monitoring and shock wave CT inversion
Authors: Wang Tao , Liu Xu , Liu Liyuan , Tang Jingxiao , Zhang Xipeng , Cao Fangshan 
Published: December 2024

Abstract
Considering the susceptibility of typical isolated working faces to intense ore pressure and the potential for rock burst disasters, this study employs micro-seismic monitoring and shock wave computed tomography (CT) inversion data from the 10304 isolated working face at Xinglongzhuang Coal Mine. The analysis focuses on the structural characteristics of the overburden rock and the evolutionary patterns of impact hazards within the isolated working face. The findings indicate that during mining operations in the vicinity of the cutting eye, the irregular coal pillar and the NF6 fault were subjected to mining-induced disturbances, which altered the stress conditions. A clustering of micro-seismic events was observed at the incision location, with events exhibiting energies surpassing 5×103 J. The reactivation of the NF6 fault within the 10303 goaf led to the rock fragmentation, resulting in a stress anomaly area within the same goaf. As the working face approached the F3 fault, the fault was influenced by the working face's disturbance, triggering fault activation. This results in extensive roof collapse, compromise of the surrounding rock's integrity, and an increase in the frequency of high-energy micro-seismic events to 756. Concurrently, a high-stress area emerged near the coal pillars on both adjacent sides of the goaf. By integrating micro-seismic monitoring, shock wave CT inversion data, and 3DEC numerical simulation outcomes, the regions of high stress concentration are precisely identified. This integrated approach allows for the assessment of impact risks and provides experience in preventing and controlling rock burst disasters.
High pyrethroid resistance is associated with high frequencies of 1014F and 1014S kdr mutations in Anopheles arabiensis (Diptera: Culicidae) from Ouagadougou, Burkina Faso
Authors: Somda Zephirin , Zanré Nicolas , Wangrawa Dimitri W , Toé Hyacinthe K , Sombié Aboubacar , Saiki Erisha , Fukumoto Shinya , Sakurai Tatsuya , Sanon Antoine , McCall Philip J , Kanuka Hirotaka , Weetman David , Badolo Athanase 
Published: December 2024

Abstract
Malaria remains a major public health threat in Burkina Faso, as in most sub-Saharan Africa countries. Malaria control relies mainly on long-lasting insecticide-treated nets (LLINs) and indoor residual spraying. In Burkina Faso, an escalating of insecticide resistance has been observed over the last decades. This study aimed to investigate insecticide resistance and the underlying mechanisms in Anopheles gambiae complex in Ouagadougou. Anopheles gambiae s.l. larvae were collected from gutters and ponds, in Zogona, Tampouy and Tanghin, 3 localities in Ouagadougou from July to October 2018. The larvae were reared in the laboratory to adults stage and susceptibility profile to pyrethroid, carbamate, and organophosphate insecticides was assessed using WHO tube assays. Mosquito species and mutations linked with insecticide resistance, were identified through PCR. More than 95% of the collected An. gambiae s.l. were An. arabiensis. An. arabiensis displayed high resistance to permethrin and deltamethrin, with mortalities below 30%, but was fully susceptible to bendiocarb, fenitrothion, and malathion. A high-frequency of the pyrethroid resistance-associated kdr mutation 1014F (0.81) was recorded, while the frequency of 1014S mutation (0.18) was lower. However, the carbamate and organophosphate-associated Ace-1 119S mutation was not detected. Localities and breeding site type appear to influence pyrethroid resistance in the An. arabiensis population of Ouagadougou. The high resistance to pyrethroids in An. arabiensis of urban Ouagadougou is underpinned, at least in part by high-frequency kdr mutations. This result supports the switch to next-generation LLINs, in well-established pyrethroid resistance zones of Burkina Faso including Ouagadougout