bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sólo Se Vive Una Vez: Evaluation of a Social Marketing Campaign Promoting HIV Screening and Prevention for Immigrant Latinxs
Authors: Shah Harita S. , Grieb Suzanne M. Dolwick , Flores-Miller Alejandra , Yenokyan Karine , Castellanos-Aguirre Jimena , Greenbaum Adena , Page Kathleen R. 
Published: February 2021

AbstractLatinxs in the U.S. are disproportionately affected by HIV and more likely to have delayed diagnosis than their non-Latinx peers. We developed and implemented Sólo Se Vive Una Vez (You Only Live Once), the first Spanish-language campaign aimed at improving HIV testing and prevention among Latinx immigrants in Baltimore, Maryland. Sólo Se Vive Una Vez featured a website (www.solovive.org) and social marketing campaign promoting free HIV testing through the Baltimore City Health Department (BCHD) clinic and Latinx outreach team. The campaign was not associated with a change in the overall number of Latinxs obtaining HIV testing. However, Latinx HIV testers who reported being exposed to the campaign had significantly higher rates of high-risk sexual behaviors, mean number of sexual partners, and substance use. The campaign was also associated with increased PrEP referrals through the BCHD Latinx outreach team.
How land-use intensity affects sexual and parthenogenetic oribatid mites in temperate forests and grasslands in Germany
Authors: Wehner Katja , Schuster Romina , Simons Nadja K. , Norton Roy A. , Blüthgen Nico , Heethoff Michael 
Published: February 2021

AbstractIntensive land use has been shown to alter the composition and functioning of soil communities. Due to their low dispersal ability, oribatid mites are particularly vulnerable to land-use intensification and species which are not adjusted to management-related disturbances become less abundant. We investigated how different land-use parameters in forests and grasslands affect oribatid mite diversity and abundance, with a focus on: (1) species-level impacts, by classifying species as increasing (‘winners’) or decreasing (‘losers’) in abundance with higher land-use intensity, and (2) reproductive impact, by investigating whether sexual and parthenogenetic species react differently. We collected 32,542 adult oribatid mites in 60 forests and grasslands of known land-use intensity in two regions of Germany. Diversity and total abundance as well as the proportion of sexual species were higher in forests than in grasslands. Diversity declined with higher land-use intensity in forests, but increased with higher mowing and fertilization in grasslands. Depending on land-use parameter and region, abundance either declined or remained unaffected by increasing intensity. Gravidity was higher in sexual than in parthenogenetic species and sexuals had 1.6× more eggs per gravid female. Proportions of sexual species and gravid females decreased with land-use intensity in forests, but increased with mowing in grasslands. At the species level, 75% of sexuals and 87.5% of parthenogens were ‘losers’ of higher percentages of dead wood originating from management-related disturbances. Across land-use parameters and habitats, a similar proportion of sexual and parthenogenetic oribatid mite species were ‘losers’ of high land-use intensity. However, ‘winner’ species were more common among sexuals.
Sum of High-Risk Gene Mutation (SHGM): A Novel Attempt to Assist Differential Diagnosis for Adrenocortical Carcinoma with Benign Adenoma, Based on Detection of Mutations of Nine Target Genes
Authors: Zheng Guo-Yang , Zhang Xue-Bin , Li Han-Zhong , Zhang Yu-Shi , Deng Jian-Hua , Wu Xing-Cheng 
Published: February 2021

AbstractThere has been no research on applying gene detection to differential diagnosis of adrenocortical carcinoma (ACC). We attempted to explore a novel auxiliary method for differential diagnosis between ACC with benign adrenocortical adenoma (ACA), based on mutations of target genes in tissues. Nine genes were chosen as target genes, including TP53, CTNNB1, ARMC5, PRKAR1A, ZNRF3, RB1, APC, MEN1, and RPL22. Exons sequencing of target genes were performed in 98 cases of tissue samples by FastTarget technology, including 41 ACC tissues, 32 ACA tissues, and 25 normal adrenal gland tissues. Significant mutations were detected and identified, and the clinical information was collected, for further comparative analysis and application to assist differential diagnosis of ACC. We identified 132 significant gene mutations and 227 significant mutation sites in 37 ACC tissues, much more than ACA and normal adrenal gland tissues. Mutation rates of 6 genes in ACC tissues were obviously higher than ACA tissues, including ZNRF3, ARMC5, TP53, APC, RB1, and PRKAR1A, regarded as high-risk genes. The sum of mutated high-risk genes detected in each sample was denominated sum of high-risk gene mutation (SHGM), and the rates of SHGM > 0 and SHGM > 1 in ACC tissues were 73.0% and 62.2%, respectively, both obviously higher than those in ACA tissues, with significant statistic differences. Especially for 8 cases of ACC with diameter < 5 cm, SHGM > 0 and SHGM > 1 were found in 6 samples (75%) and 4 samples (50%), respectively. However, no relevance was found between SHGM and clinical characteristics of ACC. We identified 6 high-risk genes in ACC tissues, with significantly higher mutation rates than ACA or normal adrenal gland tissues. The sum of mutated high-risk genes detected in ACC tissues was denominated SHGM, which was potential to assist the differential diagnosis of ACC with ACA, especially for the small-size ACC.
What mediates the racial/ethnic disparity in psychosocial stress among breast cancer patients?
Authors: Sánchez-Díaz C. T. , Strayhorn S. , Tejeda S. , Vijayasiri G. , Rauscher G. H. , Molina Y. 
Published: February 2021

Abstract
Background
Prior studies have observed greater levels of psychosocial stress (PSS) among non-Hispanic (nH) African American and Hispanic women when compared to nH White patients after a breast cancer diagnosis. We aimed to determine the independent and interdependent roles of socioeconomic position (SEP) and unmet support in the racial disparity in PSS among breast cancer patients.

Methods
Participants were recruited from the Breast Cancer Care in Chicago study (n = 989). For all recently diagnosed breast cancer patients, aged 25–79, income, education, and tract-level disadvantage and affluence were summed to create a standardized socioeconomic position (SEP) score. Three measures of PSS related to loneliness, perceived stress, and psychological consequences of a breast cancer diagnosis were defined based on previously validated scales. Five domains of unmet social support needs (emotional, spiritual, informational, financial, and practical) were defined from interviews. We conducted path models in MPlus to estimate the extent to which PSS disparities were mediated by SEP and unmet social support needs.

Results
Black and Hispanic patients reported greater PSS compared to white patients and greater unmet social support needs (p = 0.001 for all domains). Virtually all of the disparity in PSS could be explained by SEP. A substantial portion of the mediating influence of SEP was further transmitted by unmet financial and practical needs among Black patients and by unmet emotional needs for Hispanic patients.

Conclusions
SEP appeared to be a root cause of the racial/ethnic disparities in PSS within our sample. Our findings further suggest that different interventions may be necessary to alleviate the burden of SEP for nH AA (i.e., more financial support) and Hispanic patients (i.e., more emotional support).
ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer
Authors: Bhat Samatha , Kabekkodu Shama Prasada , Adiga Divya , Fernandes Rayzel , Shukla Vaibhav , Bhandari Poonam , Pandey Deeksha , Sharan Krishna , Satyamoorthy Kapaettu 
Published: February 2021

AbstractCervical cancer (CC) is a leading cause of cancer-related death among women in developing countries. However, the underlying mechanisms and molecular targets for therapy remain to be fully understood. We investigated the epigenetic regulation, biological functions, and clinical utility of zinc-finger protein 471 (ZNF471) in CC. Analysis of cervical tissues and five independent public datasets of CC showed significant hypermethylation of the ZNF471 gene promoter. In CC cell lines, promoter DNA methylation was inversely correlated with ZNF471 expression. The sensitivity and specificity of the ZNF471 hypermethylation for squamous intraepithelial lesion (SIL) vs tumor and normal vs tumor was above 85% with AUC of 0.937. High methylation and low ZNF471 expression predicted poor overall and recurrence-free survival. We identified −686 to +114 bp as ZNF471 promoter, regulated by methylation using transient transfection and luciferase assays. The promoter CpG site methylation of ZNF471 was significantly different among cancer types and tumor grades. Gal4-based heterologous luciferase reporter gene assays revealed that ZNF471 acts as a transcriptional repressor. The retroviral mediated overexpression of ZNF471 in SiHa and CaSki cells inhibited growth, proliferation, cell migration, invasion; delayed cell cycle progression in vitro by increasing cell doubling time; and reduced tumor growth in vivo in nude mice. ZNF471 overexpression inhibited key members of epithelial-mesenchymal transition (EMT), Wnt, and PI3K-AKT signaling pathways. ZNF471 inhibited EMT by directly targeting vimentin as analyzed by bioinformatic analysis, ChIP-PCR, and western blotting. Thus, ZNF471 CpG specific promoter methylation may determine the prognosis of CC and could function as a potential tumor suppressor by targeting EMT signaling.
Sensitivity of projected climate impacts to climate model weighting: multi-sector analysis in eastern Africa
Authors: Kolusu Seshagiri Rao , Siderius Christian , Todd Martin C. , Bhave Ajay , Conway Declan , James Rachel , Washington Richard , Geressu Robel , Harou Julien J. , Kashaigili Japhet J. 
Published: February 2021

AbstractUncertainty in long-term projections of future climate can be substantial and presents a major challenge to climate change adaptation planning. This is especially so for projections of future precipitation in most tropical regions, at the spatial scale of many adaptation decisions in water-related sectors. Attempts have been made to constrain the uncertainty in climate projections, based on the recognised premise that not all of the climate models openly available perform equally well. However, there is no agreed ‘good practice’ on how to weight climate models. Nor is it clear to what extent model weighting can constrain uncertainty in decision-relevant climate quantities. We address this challenge, for climate projection information relevant to ‘high stakes’ investment decisions across the ‘water-energy-food’ sectors, using two case-study river basins in Tanzania and Malawi. We compare future climate risk profiles of simple decision-relevant indicators for water-related sectors, derived using hydrological and water resources models, which are driven by an ensemble of future climate model projections. In generating these ensembles, we implement a range of climate model weighting approaches, based on context-relevant climate model performance metrics and assessment. Our case-specific results show the various model weighting approaches have limited systematic effect on the spread of risk profiles. Sensitivity to climate model weighting is lower than overall uncertainty and is considerably less than the uncertainty resulting from bias correction methodologies. However, some of the more subtle effects on sectoral risk profiles from the more ‘aggressive’ model weighting approaches could be important to investment decisions depending on the decision context. For application, model weighting is justified in principle, but a credible approach should be very carefully designed and rooted in robust understanding of relevant physical processes to formulate appropriate metrics.
Virulence tests of Neofusicoccum parvum, Lasiodiplodia theobromae, and Phytophthora palmivora on Theobroma cacao
Authors: Puig A. S. , Keith L. M. , Matsumoto T. K. , Gutierrez O. A. , Marelli J. P. 
Published: February 2021

AbstractNeofusicoccum parvum is a recently reported pathogen affecting Theobroma cacao L., and has been isolated from symptomatic pods on Oahu and Hawaii Islands. Determining infection routes and virulence are essential for assessing the impact of N. parvum on cacao production and developing effective disease management strategies. Infection routes were determined by inoculating unwounded stems and pods with six isolates of N. parvum alongside Lasiodiplodia theobromae and Phytophthora palmivora. Fifty percent of unwounded stems inoculated with P. palmivora developed lesions, but only a single lesion developed following inoculation with N. parvum (isolate H44). L. theobromae and the remaining N. parvum isolates did not induce lesion development on unwounded stems. In contrast, all N. parvum and L. theobromae isolates produced lesions on 40–100% of unwounded pods of GNV 164 and GNV 360. Low incidences of infection were observed in unwounded pods of ICS 95 (0–66.7%), SHRS 21 (0–75%), and SHRS 33 (0–20%). On wounded pods, all pathogen species produced similar size lesions, ranging from 1.90 to 7.60 cm four days after inoculation. Results from this study show that all three species can produce high rates of pod infection on some clones in the absence of wounds, but stem infection is less likely. In addition, this is the first report of L. theobromae infecting cacao pods and P. palmivora infecting stems in the absence of wounds.
Potential adaptation strategies for climate change impact among flood-prone fish farmers in climate hotspot Uganda
Authors: Oyebola Oyediran O. , Efitre Jackson , Musinguzi Laban , Falaye Augustine E. 
Published: February 2021

AbstractClimate-induced floods are increasing in Africa. The need to evolve framework for adaptation to climate change impact (CCI) among flood-prone fish farmers necessitated this study. Based on availability, 60 farming active flood-experienced fish farmers were purposively selected from flood-prone (Gulu and Kibuku) regions in climate hotspot Uganda. These were assessed for CCI adaptation tendencies in their socioeconomics and farming operation/techniques (FOI) indices, CCI awareness, CCI adaptation strategies, and perceived required interventions (RIs) using structured questionnaire. Data were analyzed through descriptive and multivariate regression tools. Respondents were mostly male, adults, educated, Christian, married, medium-sized family, social group members, 1–5 years’ experience, medium-sized farms, and government trained. Income was 1.0–6.0 million Ugx/year. In FOI, semi-intensive culture system, use of rainwater plus groundwater, small-sized (< 0.5 ha) ponds, ponds possession of inlets and outlets, seasonal farming and mixed culture/farming dominated. Most respondents were aware of CCI, while erratic rainfall with floods and prolonged drought mostly impacted farming. AS-Adaptation Strategy were changed stocking time and livelihood diversification. Communication technology and social group’s membership enhanced adaptation, while inadequate awareness constrains adaptation. Respondents’ RI(s) were tree planting, irrigation and pond perimeter trenching. Gender, government training, farm size, water sources and presence of inlet and outlets in ponds (FOI) predicted adaptation (R = 0.802, R2 = 0.64, P < 0.05). Some socioeconomic, technical and awareness indices could assist CCI flexibility. However, the statistically significant predictors of adaptation, identified adaptive strategies, constraints to adaptation and required interventions could be integrated into a framework for effective CCI adaptation for sustainable fish farming in flood-prone scenarios.
Illegal logging, governance effectiveness and carbon dioxide emission in the timber-producing countries of Congo Basin and Asia
Authors: Piabuo Serge Mandiefe , Minang Peter A. , Tieguhong Chupezi Julius , Foundjem-Tita Divine , Nghobuoche Frankline 
Published: February 2021

AbstractThe empirical link between governance and illegal logging is widely accepted amongst scientist, although a minority still purports that illegal logging does not necessarily prevail because of poor governance. However, the nexus linking governance, illegal logging and carbon emission is not well enshrined in scientific literature. This paper seeks to review the literature on illegal logging and governance and empirically investigate the effect of illegal logging and governance effectiveness on carbon emission. Using panel dynamic ordinary least square method on data covering three Congo Basin timber-producing countries and three Asian timber-producing countries, this paper further investigates disaggregated effects between these two groups of countries. The empirical evidence underscores that Congo Basin timber-producing countries are characterised by increasing trend of illegal logging, poor governance effectiveness and corruption. Panel regression reveals a positive and significant impact of illegal logging, governance effectiveness and corruption on carbon emission. Asian producing countries depict a reducing trend in illegal logging and improvements in governance and corruption. There is a positive but not significant impact of illegal logging on carbon emission, and governance effectiveness reduces carbon emission. Thus, the dynamics of governance, illegal logging and carbon emission is not the same between timber-producing countries in Asia and Congo producing counties, thus suggesting the ability of institutions to curb illegal logging and enforce laws to reduce the effects of carbon emission. Multi-stakeholder consultations, government engagement, partnerships and training of control staff can help curb corruption. Legality checks should go beyond having legal documents to effectively check and control of timber concessions and small-scale logging.
Moral Progress and Evolution: Knowledge Versus Understanding
Authors: Severini Eleonora 
Published: February 2021

AbstractThe paper explores the interplay among moral progress, evolution and moral realism. Although it is nearly uncontroversial to note that morality makes progress of one sort or another, it is far from uncontroversial to define what constitutes moral progress. In a minimal sense, moral progress occurs when a subsequent state of affairs is better than a preceding one. Moral realists conceive “it is better than” as something like “it more adequately reflects moral facts”; therefore, on a realist view, moral progress can be associated with accumulations of moral knowledge. From an evolutionary perspective, on the contrary, since there cannot be something like moral knowledge, one might conclude there cannot even be such a thing as moral progress. More precisely, evolutionism urges us to ask whether we can acknowledge the existence of moral progress without being committed to moral realism. A promising strategy, I will argue, is to develop an account of moral progress based on moral understanding rather than moral knowledge. On this view, moral progress follows increases in moral understanding rather than accumulations of moral knowledge. Whether an understanding-based account of moral progress is feasible and what its implications for the notion itself of moral progress are, will be discussed.
On the Role of a Conserved Methionine in the Na+-Coupling Mechanism of a Neurotransmitter Transporter Homolog
Authors: Zhou Wenchang , Trinco Gianluca , Slotboom Dirk J. , Forrest Lucy R. , Faraldo-Gómez José D. 
Published: February 2021

AbstractExcitatory amino acid transporters (EAAT) play a key role in glutamatergic synaptic communication. Driven by transmembrane cation gradients, these transporters catalyze the reuptake of glutamate from the synaptic cleft once this neurotransmitter has been utilized for signaling. Two decades ago, pioneering studies in the Kanner lab identified a conserved methionine within the transmembrane domain as key for substrate turnover rate and specificity; later structural work, particularly for the prokaryotic homologs GltPh and GltTk, revealed that this methionine is involved in the coordination of one of the three Na+ ions that are co-transported with the substrate. Albeit extremely atypical, the existence of this interaction is consistent with biophysical analyses of GltPh showing that mutations of this methionine diminish the binding cooperativity between substrates and Na+. It has been unclear, however, whether this intriguing methionine influences the thermodynamics of the transport reaction, i.e., its substrate:ion stoichiometry, or whether it simply fosters a specific kinetics in the binding reaction, which, while influential for the turnover rate, do not fundamentally explain the ion-coupling mechanism of this class of transporters. Here, studies of GltTk using experimental and computational methods independently arrive at the conclusion that the latter hypothesis is the most plausible, and lay the groundwork for future efforts to uncover the underlying mechanism.
Molecular insight into somaclonal variation phenomena from transcriptome profiling of cucumber (Cucumis sativus L.) lines
Authors: Pawełkowicz Magdalena Ewa , Skarzyńska Agnieszka , Mróz Tomasz , Bystrzycki Estelle , Pląder Wojciech 
Published: February 2021

AbstractSomaclonal variation during in vitro culture is often an undesirable phenomenon but may also be a source of genetic variation useful for breeders. The molecular mechanisms underlying this phenomenon remain uncertain. In this study, we analyzed the fruit transcriptome of three independent cucumber lines using RNA-sequencing technology and performed an extensive in silico study to determine how somaclonal variation altered gene expression. Comparison of the transcriptome profiles with the wild-type cucumber ‘Borszczagowski B10’ revealed 418, 364, and 273 genes that were differentially regulated. We performed bioinformatic functional analysis, gene ontology classification, molecular network analysis, and explored differentially expressed genes associated with processes such as protein and nucleic acid binding, enzyme activity, signaling, transport, sugar and lipid metabolism. We determined that the differential gene expression may be caused by polymorphism in the genic region and may also be a result of interaction among molecular networks, which triggers specific pathways.
Post-illumination activity of Bi2WO6 in the dark from the photocatalytic “memory” effect
Authors: Yang Weiyi , Chen Yan , Gao Shuang , Sang Licheng , Tao Ruoge , Sun Caixia , Shang Jian Ku , Li Qi 
Published: February 2021

AbstractPhotocatalysts with the photocatalytic “memory” effect could resolve the intrinsic activity loss of traditional photocatalysts when the light illumination is turned off. Due to the dual requirements of light absorption and energy storage/release functions, most previously reported photocatalysts with the photocatalytic “memory” effect were composite photocatalysts of two phase components, which may lose their performance due to gradually deteriorated interface conditions during their applications. In this work, a simple solvothermal process was developed to synthesize Bi2WO6 microspheres constructed by aggregated nanoflakes. The pure phase Bi2WO6 was found to possess the photocatalytic “memory” effect through the trapping and release of photogenerated electrons by the reversible chemical state change of W component in the (WO4)2− layers. When the illumination was switched off, Bi2WO6 microspheres continuously produced H2O2 in the dark as those trapped photogenerated electrons were gradually released to react with O2 through the two-electron O2 reduction process, resulting in the continuous disinfection of Escherichia coli bacteria in the dark through the photocatalytic “memory” effect. No deterioration of their cycling H2O2 production performance in the dark was observed, which verified their stable photocatalytic “memory” effect.