bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of citrulline supplementation on meta-inflammation and insulin sensitivity in type 2 diabetes: a randomized, double-blind, placebo-controlled trial
Authors: Abbaszadeh Fatemeh , Azizi Samaneh , Mobasseri Majid , Ebrahimi-Mameghani Mehrangiz 
Published: May 2021

Abstract
Background
This study aimed to examine the effects of l-citrulline (l-CIT) on low-grade inflammation (meta-inflammation) and insulin sensitivity in type 2 diabetes (T2D) patients since it has exhibited hypoglycemic and anti-inflammatory effects in most animal studies.

Methods
In this double-blind, placebo-controlled randomized clinical trial, 54 patients with T2D referred to specialized clinics of Tabriz University of Medical Sciences were assigned to l-CIT group (receiving orally one 3 g sachet of l-CIT daily before breakfast) or placebo group (receiving orally one 3 g sachet of microcrystalline cellulose daily before breakfast) for eight weeks. Serum levels of fasting blood glucose, hemoglobin A1c (HbA1c), CIT, monocyte chemoattractant protein 1 (MCP-1), interleukin-6 (IL-6), and toll-like receptor 4 (TLR-4) were determined. The quantitative insulin sensitivity check index (QUICKI) and homeostatic model assessment of β-cell function (HOMA-B) index were estimated at the baseline and post-intervention.

Results
No significant difference was observed between the studied parameters at the baseline. l-CIT supplementation significantly reduced not only serum concentrations of fasting blood glucose but also HbA1c, serum IL-6 and TLR-4 levels in the l-CIT group (p < 0.05). Additionally, at the end of the study serum levels of CIT increased significantly in l-CIT group compared to the baseline and placebo group. Fasting blood glucose concentrations and HbA1c significantly decreased after the intervention compared to the placebo. There was no significant difference in serum IL-6, TLR-4, MCP-1 levels, as well as QUICKI and HOMA-B index between the two groups, even after adjusting for baseline variables and confounders.

Conclusions
Our findings revealed that, although l-CIT supplementation significantly reduced fasting blood glucose concentrations, HbA1c and increased serum levels of CIT. It seems it could not significantly improve insulin sensitivity and meta-inflammation biomarkers. Additional studies with longer duration and different doses of l-CIT are required.
Trial registration The protocol of this clinical trial is registered at the Iranian Registry of Clinical Trials (registration no: IRCT20100209003320N16 at www.irct.ir)
Primary splenic anaplastic variant of diffuse large B-cell lymphoma: a case report
Authors: Ismail Sawsan , Ali Filip , Ajamieh Hussein , Kanaan Samir , Issa Rana , Daoud Ali , Alshehabi Zuheir 
Published: May 2021

Abstract
Background
Primary splenic lymphoma represents a rare entity that constitutes less than 1% of non-Hodgkin lymphomas, and less than 2% of all lymphomas. Diffuse large B-cell lymphoma (DLBCL) is the most common histological subtype of primary splenic lymphomas. DLBCL encompasses a heterogeneous entity with distinct morphological variants. The anaplastic variant of DLBCL was first defined in the 2017 World Health Organization classification as a rare histological subtype that constitutes less than 3.4% of DLBCL cases.

Case presentation
A 65-year-old Syrian man presented to our hospital with constant dull localized left upper quadrant abdominal pain for about 20 days accompanied by general weakness, loss of appetite, and rapid weight loss. Clinical examination revealed isolated splenomegaly and left upper abdominal tenderness. Following physical, laboratory, and radiologic examinations, the patient underwent splenectomy. Interestingly, pathological and immunohistochemical examinations of the resected spleen confirmed the diagnosis of a primary anaplastic variant of DLBCL.

Conclusions
Herein, we aimed to present an unusual combination of a rare splenic neoplasm and a unique lymphoma subtype. Furthermore, we aimed to highlight the difficulties in differential diagnosis and the importance of histological and immunohistochemical examinations with clinical correlation.
Nanopattern surface improves cultured human myotube maturation
Authors: Brunetti Jessica , Koenig Stéphane , Monnier Arthur , Frieden Maud 
Published: May 2021

Abstract
Background
In vitro maturation of human primary myoblasts using 2D culture remains a challenging process and leads to immature fibers with poor internal organization and function. This would however represent a valuable system to study muscle physiology or pathophysiology from patient myoblasts, at a single-cell level.

Methods
Human primary myoblasts were cultured on 800-nm wide striated surface between two layers of Matrigel, and in a media supplemented with an inhibitor of TGFβ receptor. Gene expression, immunofluorescence, and Ca2+ measurements upon electrical stimulations were performed at various time points during maturation to assess the organization and function of the myotubes.

Results
We show that after 10 days in culture, myotubes display numerous functional acetylcholine receptor clusters and express the adult isoforms of myosin heavy chain and dihydropyridine receptor. In addition, the myotubes are internally well organized with striations of α-actinin and STIM1, and occasionally ryanodine receptor 1. We also demonstrate that the myotubes present robust Ca2+ responses to repetitive electrical stimulations.

Conclusion
The present method describes a fast and efficient system to obtain well matured and functional myotubes in 2D culture allowing thorough analysis of single-cell Ca2+ signals.
National genotype prevalence and age distribution of human papillomavirus from infection to cervical cancer in Japanese women: a systematic review and meta-analysis protocol
Authors: Palmer Matthew , Katanoda Kota , Saito Eiko , Martellucci Cecilia Acuti , Ostuki Shiori , Nomura Shuhei , Ota Erika , Brotherton Julia M. L. , Hocking Jane 
Published: May 2021

Abstract
Background
Despite prophylactic human papillomavirus (HPV) vaccination being a safe, effective and cost-effective public health intervention for the prevention of cervical cancer, the HPV vaccine is not actively recommended or promoted by the Ministry of Health Labour and Welfare in Japan. With already very low levels of cervical screening below 30%, and vaccination levels that are below levels that award any population effect at 0.3% of the eligible population, cervical cancer mortality is higher than other similar high-income countries at 4.4/100,000 (2900) deaths per year in 2015. There is limited population-based or nationally representative data for HPV genotype distribution in Japan, thus making an assessment of the burden of vaccine-preventable cervical cancer difficult. Therefore, this systematic review and meta-analysis aims to determine the HPV genotype prevalence and age distribution of HPV infection in women with a cytological or histological diagnosis of normal through cervical cancer in Japan. We anticipate this information will guide and enhance programme interventions to reduce vaccine-preventable cervical cancer mortality in Japan.

Methods
PubMed, Embase and the Japan Medical Abstract Society Database will be searched from the date of establishment to March 2021 to identify original research articles that report the prevalence of HPV genotypes in Japanese women with normal cervical cytology, low grade, high grade and cancerous cervical lesions. No exclusion criteria relating to language or publication date will be applied. The quality of the studies will be assessed using the Joanna Briggs checklist for prevalence studies. Randomised control trials, cohort studies, cross-sectional and prevalence studies will be considered eligible. Study findings will be combined using a traditional random-effects or fixed-effects meta-analysis to summarise pooled prevalence and 95% confidence intervals depending on heterogeneity. Subgroup analyses and meta-regression will be used to investigate heterogeneity, and sensitivity analyses will be conducted to assess the robustness of the findings.

Discussion
To our knowledge, this is the first systematic review protocol that includes both Japanese and English peer-reviewed articles for the determination of genotype-specific HPV prevalence in cytological or histological confirmed normal cervical specimens, low- and high-grade intraepithelial lesions and cervical cancers by age in Japan. We anticipate this information will guide and enhance programme interventions to reduce vaccine-preventable cervical cancer mortality in Japan.

Systematic review registration
PROSPERO CRD42018117596
Diet, obesity, and the gut microbiome as determinants modulating metabolic outcomes in a non-human primate model
Authors: Newman Tiffany M. , Shively Carol A. , Register Thomas C. , Appt Susan E. , Yadav Hariom , Colwell Rita R. , Fanelli Brian , Dadlani Manoj , Graubics Karlis , Nguyen Uyen Thao , Ramamoorthy Sivapriya , Uberseder Beth , Clear Kenysha Y. J. , Wilson Adam S. , Reeves Kimberly D. , Chappell Mark C. , Tooze Janet A. , Cook Katherine L. 
Published: May 2021

Abstract

Background
The objective of this study was to increase understanding of the complex interactions between diet, obesity, and the gut microbiome of adult female non-human primates (NHPs). Subjects consumed either a Western (n=15) or Mediterranean (n=14) diet designed to represent human dietary patterns for 31 months. Body composition was determined using CT, fecal samples were collected, and shotgun metagenomic sequencing was performed. Gut microbiome results were grouped by diet and adiposity.

Results
Diet was the main contributor to gut microbiome bacterial diversity. Adiposity within each diet was associated with subtle shifts in the proportional abundance of several taxa. Mediterranean diet-fed NHPs with lower body fat had a greater proportion of Lactobacillus animalis than their higher body fat counterparts. Higher body fat Western diet-fed NHPs had more Ruminococcus champaneliensis and less Bacteroides uniformis than their low body fat counterparts. Western diet-fed NHPs had significantly higher levels of Prevotella copri than Mediterranean diet NHPs. Western diet-fed subjects were stratified by P. copri abundance (P. copriHIGH versus P. copriLOW), which was not associated with adiposity. Overall, Western diet-fed animals in the P. copriHIGH group showed greater proportional abundance of B. ovatus, B. faecis, P. stercorea, P. brevis, and Faecalibacterium prausnitzii than those in the Western P. copriLOW group. Western diet P. copriLOW subjects had a greater proportion of Eubacterium siraeum. E. siraeum negatively correlated with P. copri proportional abundance regardless of dietary consumption. In the Western diet group, Shannon diversity was significantly higher in P. copriLOW when compared to P. copriHIGH subjects. Furthermore, gut E. siraeum abundance positively correlated with HDL plasma cholesterol indicating that those in the P. copriLOW population may represent a more metabolically healthy population. Untargeted metabolomics on urine and plasma from Western diet-fed P. copriHIGH and P. copriLOW subjects suggest early kidney dysfunction in Western diet-fed P. copriHIGH subjects.

Conclusions
In summary, the data indicate diet to be the major influencer of gut bacterial diversity. However, diet and adiposity must be considered together when analyzing changes in abundance of specific bacterial taxa. Interestingly, P. copri appears to mediate metabolic dysfunction in Western diet-fed NHPs.
The precision of epidemiological investigation of COVID-19 transmission in Shanghai, China
Authors: Shi Ying , Jiang Hong-Lin , Yang Mei-Xia , Dong Lin-Juan , Chen Yue , Zhou Yi-Biao , Jiang Qing-Wu 
Published: May 2021

Abstract
Background
Shanghai had a local outbreak of COVID-19 from January 21 to 24. Timely and precise strategies were taken to prevent further spread of the disease. We discussed and shared the experience of COVID-19 containment in Shanghai.

Process
The first two patients worked at two hospitals but no staff from the two hospitals were infected. The suspected case and his two close contacts were confirmed to be infected within 12 h. The testing rate of individuals was low. The scope of screening was minimized to two related districts and the close contact tracing was completed within 12 h, which were precise and cost-effective.

Conclusions
Active monitoring, precise epidemiological investigation and timely nucleic acid testing help discover new cases, minimize the scope of screening, and interrupt the transmission.

Graphic abstract
Comparison of different methods for post-therapeutic dosimetry in [177Lu]Lu-PSMA-617 radioligand therapy
Authors: Rosar Florian , Schön Niklas , Bohnenberger Hendrik , Bartholomä Mark , Stemler Tobias , Maus Stephan , Khreish Fadi , Ezziddin Samer , Schaefer-Schuler Andrea 
Published: May 2021

Abstract
Background
Dosimetry is of high importance for optimization of patient-individual PSMA-targeted radioligand therapy (PSMA-RLT). The aim of our study was to evaluate and compare the feasibility of different approaches of image-based absorbed dose estimation in terms of accuracy and effort in clinical routine.

Methods
Whole-body planar images and SPECT/CT images were acquired from 24 patients and 65 cycles at 24h, 48h, and ≥96h after administration of a mean activity of 6.4 GBq [177Lu]Lu-PSMA-617 (range 3–10.9 GBq). Dosimetry was performed by use of the following approaches: 2D planar-based dosimetry, 3D SPECT/CT-based dosimetry, and hybrid dosimetry combining 2D and 3D data. Absorbed doses were calculated according to IDAC 2.1 for the kidneys, the liver, the salivary glands, and bone metastases.

Results
Mean absorbed doses estimated by 3D dosimetry (the reference method) were 0.54 ± 0.28 Gy/GBq for the kidneys, 0.10 ± 0.05 Gy/GBq for the liver, 0.81 ± 0.34 Gy/GBq for the parotid gland, 0.72 ± 0.39 Gy/GBq for the submandibular gland, and 1.68 ± 1.32 Gy/GBq for bone metastases. Absorbed doses of normal organs estimated by hybrid dosimetry showed small, non-significant differences (median up to 4.0%) to the results of 3D dosimetry. Using 2D dosimetry, in contrast, significant differences (median up to 10.9%) were observed. Regarding bone metastases, small, but significant differences (median up to 7.0%) of absorbed dose were found for both, 2D dosimetry and hybrid dosimetry. Bland-Altman analysis revealed high agreement between hybrid dosimetry and 3D dosimetry for normal organs and bone metastases, but substantial differences between 2D dosimetry and 3D dosimetry.

Conclusion
Hybrid dosimetry provides high accuracy in estimation of absorbed dose in comparison to 3D dosimetry for all important organs and is therefore feasible for use in individualized PSMA-RLT.
A comparison of food portion size estimation methods among 11–12 year olds: 3D food models vs an online tool using food portion photos (Intake24)
Authors: Bradley Jennifer , Rowland Maisie K. , Matthews John N. S. , Adamson Ashley J. , Spence Suzanne 
Published: May 2021

Abstract
Background
Technology has advanced bringing new cost-effective methods to measure food intake. The aim of the study was to compare food and drink portion estimates from a traditional portion estimation method using 3D food models with portion estimates using an online dietary recall tool, Intake24.

Methods
11-12 year old children were recruited from secondary schools in Newcastle upon Tyne. Each pupil completed a two-day food diary followed by an interview during which pupils estimated food portion sizes using a range of 3D food models. They also completed Intake24 for the same 2 days. Bland Altman analyses were used to compare mean intake for each method.

Results
Seventy pupils completed both portion estimation methods. There was good agreement in food weight estimations between the two methods (geometric mean ratio 1.00), with limits of agreement ranging from minus 35% to plus 53%. Intake24 provided estimates of energy intake that were 1% lower on average than estimates of energy intake using the food models. Mean intakes of all macro and micronutrients using Intake24 were within 6% of the food model estimates.

Conclusions
The findings suggest that there was little difference in portion estimations from the two methods, allowing comparisons to be made between Intake24 data and food diary data collected from same age pupils using 3D food models in previous years.
Accelerated transcranial magnetic stimulation (aTMS) to treat depression with treatment switching: study protocol of a pilot, randomized, delayed-start trial
Authors: Tan Xiao Wei , Abdin Edimansyah , Tor Phern Chern 
Published: May 2021

AbstractBackgroundRepetitive transcranial magnetic stimulation (rTMS) is a technique for stimulating brain activity using a transient magnetic field to induce an electrical current in the brain producing depolarization of focal groups of brain cells. TMS is a protocol approved by the U.S. Food and Drug Administration in routine clinical practice as a treatment for depression. A major limitation of rTMS is the large amount of time taken for a standard protocol (38 min a day for 20–30 working days). The optimal type and duration of TMS are still uncertain, as is the optimal strategy for continuing or changing the type of rTMS if there is a poor initial response.ObjectivesThe trial aims to assess whether a 1-week compressed course of left dorsolateral prefrontal (L DLPFC) 5 Hz accelerated rTMS (aTMS) treatment is as effective as an established 4-week course of non-accelerated rTMS and if additional 5 Hz L DLPFC aTMS treatments will be efficacious in non-responders as compared to 1 Hz right DLPFC aTMS treatment.MethodsA randomized, single-blind, delayed-start trial was planned to commence in Jan 2020. A total of 60 patients will be enrolled from the Institute of Mental Health Singapore within a 2-year period and randomized into the early or delayed-start phase of the trial. The primary outcome of the trial is the improvement of Montgomery-Asberg Depression Rating scale at the end of the active treatment phase.DiscussionIf this study protocol proves to be effective, the findings of this trial will be updated to the College of Psychiatrists, Academy of Medicine Singapore, as well as published in a peer-reviewed journal to enhance local and international TMS treatment guidelines.Trial registrationClinicalTrials.gov ID:NCT03941106
Quantitative characterization of siliceous contents of different origins by use of logging analysis
Authors: Wang Jianguo , Gu Daihong , Guo Wei , Zhang Haijie , Yang Daoyong 
Published: May 2021

We have developed a new and pragmatic technique to quantitatively characterize the silica content of shale of various origins. By comparing the genesis of silica in shale (i.e., biogenic origin, terrigenous origin, and clay transformation), the corresponding silica content per unit volume is determined as a function of the total silica content, each of which is determined from core samples. Subsequently, we have developed a new inverse framework and successfully applied it to quantify different types of silica in the Silurian Longmaxi Formation shale in the Zhaotong area. The biogenic silica content per unit volume is found to be up to 5.61 wt%–5.67 wt%, accounting for 12.8–14.5 times of abiogenic silica, which is approximately 0.39 wt%–0.44 wt%, indicating that biogenic silica contributes more to the total silica than that of abiogenic silica in the bottom of the Longmaxi Formation. Using well-logging data, the biogenic silica content is found to follow the same pattern as the total silica content in the vertical direction. Such an identified pattern together with the silica content leads to more accurate determination of porosity and more appropriate placement of a horizontal well with multistage fractures in a shale gas reservoir.
Petrophysical and geomechanical characterization of the Late Cretaceous limestone reservoirs from the Southeastern Constantine Basin, Algeria
Authors: Baouche Rafik , Sen Souvik , Ganguli Shib Sankar , Boutaleb Khadidja 
Published: May 2021

We have characterized the petrophysical and geomechanical properties of the Late Cretaceous Turonian and Cenomanian carbonate reservoirs from the southeast Constantine Basin, northern Algeria. In general, Turonian carbonates exhibit a wide range of porosities (2%–15%) and permeabilities (0.001–10 mD), whereas the Cenomanian reservoir appears to be very tight (<6% porosity and <0.1 mD permeability). Based on their storage and hydraulic flow characteristics, these carbonates were classified into two distinct reservoir rock types (RRT): RRT-I is hosted by nano- to microporosities that displays poor reservoir qualities compared to the RRT-II, consisting of mesoporous Turonian intervals (>10% porosity and 0.5–10 mD permeability). The reservoir pore-pressure gradient is interpreted to be a little above the hydrostatic (0.51 psi/ft), whereas the minimum horizontal stress ([Formula: see text]) has a 0.72 psi/ft gradient. In situ stress analysis establishes a dominant strike-slip tectonic stress field in the basin. Shale intercalations associated with the carbonate facies are characterized by comparatively high failure pressure that can lead to wellbore failures, which may be avoided considering the recommended minimum drilling mud weight as obtained from the rock failure criterion. Extensive wellbore breakouts (C-quality) were observed in the acoustic image logs recorded in the studied reservoir intervals, inferring a mean maximum horizontal stress azimuth of 350°N. We recommend that deviated wells in the direction of the interpreted [Formula: see text] orientation (approximately east–west) using hydraulic fracturing can be useful to attain optimum wellbore stability and effective permeability enhancement. Our findings have significant implications for enhanced production within the tight carbonate reservoirs situated in a strike-slip domain.
Neural cell adhesion molecule is required for ventricular conduction system development
Authors: Delgado Camila , Bu Lei , Zhang Jie , Liu Fang-Yu , Sall Joseph , Liang Feng-Xia , Furley Andrew J. , Fishman Glenn I. 
Published: May 2021

ABSTRACT
The most distal portion of the ventricular conduction system (VCS) contains cardiac Purkinje cells (PCs), which are essential for synchronous activation of the ventricular myocardium. Contactin-2 (CNTN2), a member of the immunoglobulin superfamily of cell adhesion molecules (IgSF-CAMs), was previously identified as a marker of the VCS. Through differential transcriptional profiling, we discovered two additional highly enriched IgSF-CAMs in the VCS: NCAM-1 and ALCAM. Immunofluorescence staining showed dynamic expression patterns for each IgSF-CAM during embryonic and early postnatal stages, but ultimately all three proteins became highly enriched in mature PCs. Mice deficient in NCAM-1, but not CNTN2 or ALCAM, exhibited defects in PC gene expression and VCS patterning, as well as cardiac conduction disease. Moreover, using ST8sia2 and ST8sia4 knockout mice, we show that inhibition of post-translational modification of NCAM-1 by polysialic acid leads to disrupted trafficking of sarcolemmal intercalated disc proteins to junctional membranes and abnormal expansion of the extracellular space between apposing PCs. Taken together, our data provide insights into the complex developmental biology of the ventricular conduction system.