bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health Problems Among Forcibly Displaced Myanmar Nationals (FDMNs) Admitted To The Medicine Ward of Cox’s Bazar Medical College Hospital
Authors: Ismail Mohammad , Hussain Mohammad Farhad , Hasan Mohammad Abdullah Al , Kamal AHM Mustafa , Rahman Monjur , Hassan Mohammad Jahid 
Published: December 2021

Abstract

Background
Forcibly displaced Myanmar nationals (FDMNs) or Rohingya refugees are one of the vulnerable groups suffering from different kinds of health problems but have been less reported yet. Therefore, the study was designed to delineate the health problems among FDMNs admitted to Cox’s Bazar Medical College Hospital.
Methods
This hospital-based cross-sectional study was conducted at the Medicine ward, Cox’s Bazar Medical College Hospital, for a six-month period following approval. Rohingya refugees who were admitted during the study period were approached for inclusion. Informed written consent was ensured prior to participation. A structured questionnaire was used during data collection. Collected information was recorded in case record form. A total of 290 subjects were interviewed. Analysis was performed using the statistical package for social science (SPSS) version 20.
Results
The mean age of the participants was 48.76±18.67 years (range: 16-91), with a clear male predominance (60.7%). Family size ranged 6-8. All of the participants reported at least one of the illnesses. Of all, 29.66% patients reported disease of the respiratory system, and 26.9% reported disease of the gastrointestinal disease and hepatobiliary system. Accidental injury or injury due to electrocution or thin falls or snake bites was present in 10.4% of the cases. Among the single most common diseases, COPD (20%) was the most frequently observed, and the rest of them were chronic liver disease (13.1%), pulmonary TB (5.5%), ischemic stroke (5.5%), CAP (4.1%), acute coronary syndrome (3.4%), thalassaemia (3.4%) and hepatocellular carcinoma (3.4%). Among the top 6 reported diseases, PTB was more common in elderly individuals (p=0.29). The disease pattern was similar across the sexes among the refugees except community acquisition pneumonia (CAP), which was commonly observed among males (p=.004). Considering different age groups, genitourinary problems were more common in males aged >60 years, and rheumatology and musculoskeletal problems were equally affected in females aged between 40-60 years.
Conclusion
COPD, CLD and CAP were the most prevalent diseases in FDMN patients who attended the medicine ward of Cox’s Bazar Medical College Hospital. Further exploration is warranted before any policy making and comprehensive plan.
Metformin Combined with Local Irradiation Provokes Abscopal Effects in a Murine Rectal Cancer Model
Authors: Tojo Mineyuki , Miyato Hedeyo , Koinuma Koji , Horie Hisanaga , Tsukui Hidenori , Kimura Yuki , Kaneko Yuki , Ohzawa Hideyuki , Yamaguchi Hironori , Yoshimura Kotaro , Lefor Alan Kawarai , Sata Naohiro , Kitayama Joji 
Published: December 2021

Abstract
BackgroundAlthough preoperative chemoradiation therapy can down-stage locally advanced rectal cancer (LARC), it has little effect on distant metastases. Metformin exerts an anti-cancer effect partly through the activation of host immunity. MethodLuM1, a highly lung metastatic subclone of colon 26, was injected subcutaneously in BALB/c mice and treated with metformin and/or local radiation (RT). Lung metastases and the primary tumor were evaluated and the phenotypes of immune cells in the spleen and lung metastases were examined with flowcytometry and immunohistochemistry.ResultsLocal RT, but not metformin, partially delayed the growth of sc tumor which was augmented with metformin. Lung metastases was unchanged in metformin or RT alone, but significantly reduce in the combined therapy. The ratios of splenic T cells tended to be low in the RT group, which were increased by the addition of metformin. IFN-gamma production of the splenic CD4(+) and CD8(+) T cells was enhanced and CD49b (+) CD335(+) activated NK cells was increased after combined treatment group. Density of NK cells infiltrating in lung metastases was increased after combination treatment. ConclusionMetformin effectively enhances local and abscopal effects of RT though the activation of cell-mediated immunity and might be clinically useful for LARC.
Impact of Post Traumatic Stress Disorder on Employee Job Performance: Moderating Role of Spirituality
Authors: Baloch Qadar Bakhsh , Baloch Verdah Qadar , Maher Sourath , Iqbal Nadeem , Shah Syed Naseeb , Hussain Mujahid 
Published: December 2021

Abstract
The aim of this paper was to determine the correlation amid post-traumatic stress disorder (PTSD) and employee job performance with moderating role of spirituality among Police officials who experienced during the war on terror. Ninety seven personnel from Pakistan Security Forces, hospitalized as result of injuries in war against terror, were selected as study respondents and personally administered with the seventeen items questionnaire to measure PTSD symptoms. All the instruments administered to measure PTSD symptoms, employees’ performance and spiritualty were adopted. Andrew F. Hayes Process model was used to test the hypotheses derived from the study’s conceptual model as it was identical with the Andrew model (Hayes 2013) in which moderation is tested with one moderator that is spirituality. Correlation and Regression analysis were carried out by using SPSS. Moderation analysis checked the moderating effect of spirituality upon the relationship between PTSD and employees performance. The research concluded that PTSD negatively affects the job performance, however, spirituality reduces the negative outcomes of PTSD. The research found that the spirituality contributes positively to the psychological well-being of a person, thereby making one’s self more robust to tremor and harrowing engagement and subsiding the negative impacts of PTSD. Managerial implications and future direction were also discussed.
Recognizing relationship between groundwater level and hydrological time series (Case study: Ardabil plain)
Authors: Vousoughi Farnaz Daneshvar 
Published: December 2021

Abstract
Two approaches to identify the relation between hydrological time series (rainfall and runoff) and groundwater level (GWL) were used in the Ardabil plain. In this way, Wavelet-entropy measure (WEM) and wavelet transform coherence (WTC) as two approaches of wavelet transform (WT) were used. WEM have been considered as a criterion for the degree of time series fluctuations and WTC present common time-frequency space. In WEM calculation, monthly rainfall, runoff and GWL time series were divided into three different time periods and decomposed to multiple frequent time series and then, the energies of wavelet were calculated for each sub-series. The result showed WEM reduction in rainfall, runoff and GWL. The reduction of WEM presents the natural fluctuations decrease of time series. The reduction of entropy for runoff, rainfall and GWL time series were about 1.58, 1.36 and 29% respectively, it is concluded that fluctuation reduction of hydrological time series has relatively not more effect on the oscillation patterns of GWL signal. In this regard, it could be concluded that the human activities such as water driving from wells can be played main role in the reduction of GWL in Ardabil plain. WTC findings showed that runoff had most coherence (0.9-1) among the hydrological variables with GWL time series in the frequency bands of 4-8 and 8-16 months.
Evaluation of the Virulence Factor Profile of Avian Pathogenic Escherichia Coli in Clinical Isolates of Avian Samples in Caloto, Cauca, Colombia
Authors: Durango Harold , Morales Hernando , Murillo Enderson , Echeverri Laura , Quiroz Natalia , Villarreal Rafael 
Published: December 2021

Abstract
Avian pathogenic E. coli (APEC), produces an extraintestinal infection in chickens, turkeys, and other types of birds, called colibacillosis, and is considered one of the main causes of economic losses due to morbidity, mortality, and the disposal of poultry carcasses. The objective of the present study was to characterize the genetic profile of the virulence factors of different isolates of avian E. coli in Caloto, Cauca, Colombia. Materials and methods: E. coli was isolated and identified by biochemical tests, from 47 clinical isolates. Subsequently, the DNA was extracted using Chelex. Three multiplex PCRs were designed to amplify 13 virulence factors (iroN, hlyF, iss, iutA, frz, vat, sitA, KpsM, sitD, fimH, pstB, sopB, and uvrY), using primers previously reported for each. The amplification products were verified on agarose gels. Each isolate was classified according to the number of virulence factors: group A (between 10 and 13), group B (between 5 and 9), and group C (4 or less). Conclusion: we were able to identify the presence of a group of virulence factors in clinical isolates of APEC, which allows us to demonstrate that both the frequency and the profile of virulence factors in the isolated strains showed a different profile than the reported by other authors. The virulence genes pstB and fimH were detected in all of our samples, and the iss gene was the one with the lowest frequency. And according to the number of virulence factors, the group A was the most frequent.
The Biological Behavior of High-Grade Glioma in H3K27M Mutant Circumscribed Midline Gliomas
Authors: Li Hainan , Tan Jieyun , Lai Mingyao , Chen Wendan , Liu Minting , Zhou Zhaoming , Deng Dabiao , Cheng Baijie , Fan Chongzuo , Cai Linbo , Li Zhi 
Published: December 2021

Abstract
Purpose Due to the prognosis of circumscribed middle gliomas（CMGs）with H3K27M mutation is still unclear. This study explored the prognostic stratification of （CMGs） with H3K27M mutation.Methods: One hundred and sixty middle gliomas（MGs）were identified over 10 years. Immunohistochemistry was done for H3K27M, ATRX, IDH1, and P53, and Sanger sequencing performed for IDH and H3 genes. The clinicopathological characteristics were reviewed and survival analysis performed.Results: 1. H3K27M mutation was associated with a poor prognosis (p = 0.00017) for brainstem gliomas, but not for thalamic gliomas (p = 0.3). In the elder adults (≥40 years), there was no correlation between H3K27M mutation and the prognosis for MGs (p = 0.49).2. For H3K27M mutant MGs, there was no difference in prognosis between diffuse midline gliomas (DMGs) and CMGs (p = 0.211), also between the CNS WHO grades.3. The prognosis of H3K27M mutant CMGs of CNS WHO grade 1 was worse than that of H3K27M wild-type CMGs (p = 0.0024), even worse than of DMGs without H3K27M mutation of CNS WHO grade 2(p = 0.0066). There was no significant difference in prognosis from DMGs with histological grades CNS WHO grade 3 and 4 (p = 0.46).Conclusions: The weight value of H3K27M affecting prognosis is affected by location and age. CMGs with H3K27M mutation have biological behavior similar to high-grade gliomas. It is recommended that Treatment management was referenced to high-grade glioma for CMGs with H3K27 mutation.
The Mammalian Kalrn Locus Gives Rise to Several Novel Long Non-coding RNAs
Authors: Pal Muneesh , Chaubey Divya , Tanwar Mohit , Pillai Beena 
Published: December 2021

Abstract
The Kalrn gene encodes several multi-domain protein isoforms that localise to neuronal synapses, and play dynamic roles in shaping axonal outgrowth, dendrite morphology and dendritic spine re-modelling. The genomic locus is implicated in several neurodevelopmental and neuropsychiatric diseases including autism, schizophrenia and bipolar disease. Mutations in the coding regions, inherited in a classical Mendelian manner, have also been implicated in certain forms of autism and intellectual disability. At the molecular level, the protein isoforms, encoded by reported transcript isoforms, share some core domains arising from the central exons, while other domains, especially towards the C terminal may be selectively incorporated. This heterogeneity seems to confer the ability to grow and retract dendritic spines, thus making Kalirin a critical and dynamic player in dendritogenesis. We have previously shown that in the zebrafish genome, a novel brain specific non-coding RNA arising from the 5’ end of the Kalirin gene, durga regulates neuronal morphology. In search of the mammalian equivalent, we characterized the mammalian Kalrn loci in detail, annotating multiple novel non-coding RNAs, including linear and circular variants, through analysis of transcriptomics data and experimental approaches. By comparing the mouse and human loci and studying the expression of the novel lncRNAs arising from the locus during differentiation of primary cortical neurons in culture, we show that certain non-coding RNAs arising from the locus show a temporal expression profile that coincides with a subset of Kalirin protein coding isoforms. In humans, mouse and zebrafish the 5’end of the Kalrn locus gives rise to a chromatin associated lncRNA that is present in adult ovaries besides being expressed during brain development and in certain regions of the adult brain. Besides correcting some of the annotations available in public databases, we propose that this lncRNA arising from the 5’end of the Kalrn locus is the mammalian ortholog of zebrafish lncRNA durga.
Long-term Changes In Aberrant DNA Methylation And Gastritis After Helicobacter Pylori Eradication Focused On Metachronous Gastric Cancer
Authors: Tanaka Ikko , Ono Shoko , Watanabe Yoshiyuki , Yamamoto Hiroyuki , Oikawa Ritsuko , Matsumoto Shogo , Kubo Marina , Nishimura Yusuke , Shimoda Yoshihiko , Ono Masayoshi , Yamamoto Keiko , Sakamoto Naoya 
Published: December 2021

Abstract
BackgroundA persistently high methylation level in gastric mucosa after Helicobacter pylori (H. pylori) eradication is presumed to be a risk for metachronous gastric cancer (MGC); however, long-term changes in aberrant DNA methylation and histological gastritis have been unclear. Our aim was to examine changes in DNA methylation and histological gastritis according to the occurrence of MGC.MethodsSubjects were classified into 3 groups: 25 patients in whom metachronous gastric cancers occurred after the initial endoscopic resection (ER) for early gastric cancer and H. pylori eradication (MGC group), 17 patients in whom MGC did not occur for more than 5 years after initial ER and H. pylori eradication (non-MGC group) and 29 patients without a history of gastric cancer who succeeded in eradication more than 5 years ago (HP group). Aberrance of DNA methylation in 3 genes (miR-124a-3, EMX1, NKX6-1) and histological score of atrophy and intestinal metaplasia (IM) were evaluated using biopsy samples before and more than a mean of 5 years after H. pylori eradication.ResultsThe methylation level of miR-124a-3 in the HP group and non-MGC group and that of EMX1 in the HP group significantly decreased in the long term after eradication. In the MGC group, H. pylori eradication did not improve aberrant methylation, and the Z-score significantly increased. There were significant positive correlations between methylation levels in miR-124a-3 and EMX1 and histological findings after eradication.ConclusionsA persistently high methylation level after H. pylori eradication reflected precancerous mucosal conditions and led to long-term MGC.
Compare the Similarity between Two Quantum Images
Authors: Liu You-hang , Qi Zai-dong , Liu Qiang 
Published: December 2021

Abstract
Comparing the similarity between digital images is an important subroutine in various image processing algorithms. In this study, we present three quantum algorithms for comparing the similarity between two quantum images. These algorithms apply to binary, grey and color images respectively. Without considering the image preparation, the proposed algorithms achieve exponential acceleration than the existing quantum and classical methods in complexity. At the end of this paper, an experiment based on the real quantum computer of IBMQ and simulations verify the effectiveness of the algorithms.
Knockdown of CDC20 Promotes Adipogenesis of Bone Marrow-derived Stem Cells
Authors: Du Yangge , Liu Yunsong , Zhou Yongsheng , Zhang Ping 
Published: December 2021

Abstract
Background: Bone is a rigid organ that provides support and physical protection to vital organs of the body. Several bone loss disorders are commonly associated with increased bone marrow adipose tissue. Bone marrow mesenchymal stromal/stem cells (BMSCs) are multipotent progenitors differentiating into osteoblasts, adipocytes, and chondrocytes. CDC20 is a co-activator of APC/C, required for full ubiquitin ligase activity. In our previous study, CDC20 promoted the osteogenic commitment of BMSCs and Cdc20 conditional knockout mice suggested a decline in bone mass. In this study, we investigated the function of CDC20 in the adipogenic differentiation of BMSCs and provided a new clue between adipogenesis and osteogenesis. Methods: Lentivirus containing CDC20 shRNA was used for CDC20 knockdown in hBMSCs. Primary mBMSCs were isolated from Cdc20f/f and Sp7-Cre;Cdc20f/f mice. Adipogenesis was examined by qRT-PCR and western blot analysis of adipogenic regulators, Oil Red O staining and transplantation into nude mice. The CDC20 knockout efficiency was determined through immunochemistry, qRT-PCR and western blot of bone marrow. Accumulation of adiposity was measured through histology and staining of bone sections. Results: CDC20 expression in hBMSCs was significantly decreased during adipogenic differentiation. Knockdown of CDC20 enhanced adipogenic differentiation of hBMSCs in vitro. CDC20-knockdown hBMSCs showed more adipose tissue–like constructs in H&E staining and Oil Red O staining. Sp7-Cre;Cdc20f/f mice presented increased adipocytes in bone marrow compared with control mice. mBMSCs from Sp7-Cre;Cdc20f/f mice exerted upregulated adipogenic differentiation. Conclusions: Our findings showed that knockdown of CDC20 enhanced adipogenesis of h(m)BMSCs in vitro and in vivo. Overall, CDC20 regulated both adipogenesis and osteogenesis of BMSCs, and may lead to the development of new therapeutic target for “fatty bone” and osteoporosis.
Activation of TRESK background potassium channels by cloxyquin exerts protective effects against excitotoxic-induced brain injury and neuroinflammation in neonatal rats
Authors: Dilek Mustafa , Kilinc Yasemin Baranoglu , Kilinc Erkan , Torun Ibrahim Ethem , Saylan Aslihan , Duzcu Selma Erdogan 
Published: December 2021

Abstract
The excitotoxicity is a common pathological mechanism of perinatal brain injuries (PBI), however neuroinflammation resulting in PBI is both a cause and a consequence of excitotoxicity. TRESK background potassium channels are an important regulator of neuronal excitability. We therefore investigated effects of activation of TRESK channels by selective activator cloxyquin on excitotoxic-induced brain injury and neuroinflammation involving brain mast cells and inflammatory cytokines in neonatal rats. An excitotoxic model mimicking human perinatal brain lesions was established via intracerebral injection of the glutamatergic agonist ibotenate to into newborn rats. P5 rat pups were intraperitoneally pretreated with vehicle, three different doses of cloxyquin (0.2, 1 and 5 mg/kg), or NMDA receptor antagonist MK-801 (positive control) 30 minutes prior to intracerebral injection of 10 µg ibotenate. Rat pups were sacrificed one or five days after the injury. Coronal brain sections were stained with cresyl-violet for histopathological examinations, and with toluidine-blue for brain mast cells assessments. Concentrations of activin A, IL-1β, IL-6 and IL-10 in brain homogenates were measured using ELISA. Cloxyquin dose-dependently ameliorated ibotenate-induced impairments in the cortical and white matter, and suppressed ibotenate-induced activation and number of brain mast cells. Moreover, cloxyquin dose-dependently reduced concentrations of activin A, IL-1β and IL-6 in the brain tissue induced by ibotenate while it elevated IL-10 level. Our findings reveal for the first time that cloxyquin, a selective activator of TRESK channels, dose-dependently exerted protective effects against excitotoxic-induced neonatal brain injury and neuroinflammation. TRESK channels may be a promising new target for the treatment of PBIs.
Identification of HOX Signatures Contributing to Oral Cancer Phenotype
Authors: Padam Kanaka , Morgan Richard , Hunter Keith , Chakrabarty Sanjiban , Kumar Naveena , Radhakrishnan Raghu 
Published: December 2021

Abstract
Purpose: Evolutionarily conserved homeobox-containing HOX genes as transcriptional regulators in the developmental specification of organisms is well known. The contribution of HOX genes involvement in oral cancer phenotype has yet to be fully ascertained.Methods: GEO datasets (GSE72627, GSE30784, GSE37991) were accessed and analyzed using GEO2R. TCGA-HNSC HTSeq-counts and clinical data were retrieved from the GDC portal for oral cavity neoplasms. Differential HOX gene expression was profiled using the DESeq2 R package with a log2 fold change cut-off (-1 and +1) and Benjamini-Hochberg p-adjusted value at <0.01. Gene set over-representation analysis and semantic analysis associated with the disease ontology were performed using ClusterProfiler R package and pathway over-representation analysis was performed using IMPaLa. HOX protein interaction network was constructed using the Pathfind R package. HOX phenotype associations were performed using Mammalian Phenotype Ontology, Human Phenotype Ontology, PhenGenI associations, Jensen tissues, and OMIM entries. Drug connectivity mapping was carried out with Dr. Insight R Package.Results: HOXB2 and HOXA5 genes were upregulated in oral dysplasia but silenced during tumor progression. Loss of HOXB2 expression was consistent through potentially malignant dysplastic oral lesions (PMOL) to primary tumor formation. HOXA10, HOXB7, HOXC6, HOXC10 and HOXD10 showed consistent upregulation from premalignancy to malignancy and were notably associated with risk factors. Overrepresentation analysis suggested HOXA10 was involved in the transcriptional misregulation leading to oral cancer phenotype. HOX subnetwork analysis showed crucial interactions with cell cycle regulators, growth responsive elements, and proto-oncogenes.Conclusion: Phenotype associations specific to the oral region involving HOX genes provide intrinsic cues to tumor development. The 5’ HOX genes were aberrantly deregulated which reflects their posterior prevalence during oral carcinogenesis.