bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dysfunction of apoptosis and autophagy correlates with local recurrence in esophageal squamous cell carcinoma after definitive chemoradiation
Authors: Qiu Hu , Song Haixia , Luo Man , Ke Shaobo , Shi Wei , Chen Jiamei , Zhao Wensi , Luo Hesheng , Chen Yongshun 
Published: September 2021

Abstract
Objective
Definitive chemoradiotherapy (dCRT) is one of the standard treatments for esophageal squamous cell carcinoma (ESCC), but local recurrence is the main cause of treatment failure. The changes in apoptosis and autophagy in recurrent tumors of patients with ESCC following dCRT have been poorly estimated. Thus, this study aimed to investigate the expressions of key regulators of apoptosis and autophagy in matched paired samples of primary and recurrent ESCC.

Methods
The medical records of patients with locally advanced ESCC who developed local recurrence after dCRT were reviewed, and the expression profiling of apoptosis-related genes, cell apoptosis, autophagy and autophagy-related proteins were detected in normal esophageal squamous epithelium and paired samples of primary and recurrent ESCC.

Results
A total of 126 patients were enrolled, and 52.4% of them had stage III disease. The 1-, 3- and 5-year local recurrence-free survival (LRFS) rates were 54.8, 19.8 and 14.3%, respectively, with a median LRFS of 13.0 months. Patients with T2 tumor or stage II disease showed a significantly prolonged LRFS compared with that of patients with T3-4 tumor or stage III disease. The Apoptotic Machinery key genes expression profiling identified 5 upregulated and 7 downregulated apoptosis-related genes in recurrent tumors compared with their expression levels in the matched primary ESCC tumors. High expression of CD40, TRAF4 and BCL2A1, and low expression of CARD6 and TNFRSF21 were associated with increased risk of early local recurrence after dCRT. No differences in apoptotic index between primary and recurrent samples were detected. However, typical morphological features of autophagosomes and elevated LC3-II protein expression were detected in recurrent tumor samples, and positive LC3-II expression was correlated with increased risk of early local recurrence.

Conclusion
Our findings indicated that apoptosis and autophagy dysfunction correlated with early local recurrence in patients with locally advanced ESCC receiving dCRT. Further studies are necessary to understand the biology of tumor recurrence in esophageal cancer.
Immune classifier-based signatures provide good prognostic stratification and predict the clinical benefits of immune-based therapies for hepatocellular carcinoma
Authors: Xue Chen , Gu Xinyu , Li Lanjuan 
Published: September 2021

Abstract
Background
Hepatocellular carcinoma (HCC) is an aggressive cancer with a high rate of death globally. The use of bioinformatics may help to identify immune cell-related genes both as targets for potential immunotherapies and for their value associated with predicting therapy responses.

Methods
In this study, mRNA expression profiles of HCC samples from The Cancer Genome Atlas (TCGA) database were subjected to gene enrichment, cell type abundance, immune cell infiltration, and pathway enrichment analyses to determine immune cell gene features, cell type abundance, and functional annotation characteristics. We also evaluated their prognostic values using Cox regression and Kaplan–Meier analyses and assessed potential responses to chemotherapy. Four subgroups (Groups 1–4) were identified. Group 4 was associated with advanced clinical characteristics, high immune cell enrichment scores, and the poorest outcomes.

Results
Differentially expressed genes (DEGs) in the HCC samples were enriched in the following pathways: antigen binding, cell surface receptor signal transduction of the immune response, and cell surface activated receptor signal transduction of the immune response. Highly expressed genes in Group 4 were enriched in elements of the WNT signalling pathway. We identified five immune-related genes (SEMA3A, TNFRSF11B, GUCA2A, SAA1, and CALCR) that were significantly related to HCC prognosis. A prognostic model based on these five genes exhibited good predictive value, with 1-year and 5-year area under the curve (AUC) values of  >  0.66. Group 4 was also potentially more sensitive to EHT 1864, FH535, and lapatinib chemotherapies than the other groups.

Conclusions
We identified and validated four HCC subgroups based on immune system-related genes and identified five genes that may be used for an immune-based prognostic model for HCC treatment.
Evaluation of Xpert GBS assay and Xpert GBS LB assay for detection of Streptococcus agalactiae
Authors: Han Meng-Yi , Xie Chen , Huang Qing-Qing , Wu Qiao-Hua , Deng Qing-Yun , Xie Tian-Ao , Liu Ye-Ling , Li Zhuo-Lei , Zhong Jing-Hua , Wang Yan-Chao , Guo Xu-Guang 
Published: September 2021

Abstract
Background
Group B Streptococcal (GBS) infection is the primary agent of neonatal morbidity and mortality. Rapid and simple methods to detect GBS are Xpert GBS and GBS LB assays based on real-time polymerase chain reaction (PCR). However, since the diagnostic accuracy of the two techniques in diagnosing GBS remains unclear, we designed this study to appraise the diagnostic accuracy of the aforementioned.

Methods
A systematic search of all literature published before July 16, 2020 was conducted using Embase, PubMed, Web of Science, and Cochrane Library. The study quality was evaluated through Review Manager 5.3. Accordingly, data extracted in the included studies were analyzed using Meta-DiSc 1.4 and Stata 12.0 software. The diagnosis odds ratio (DOR) and bivariate boxplot were utilized to evaluate the heterogeneity. Publication bias was appraised by using Deeks’ funnel plot.

Results
A total of 13 studies were adopted and only 19 sets of data met the criteria. The sensitivity and specificity of Xpert GBS were 0.91 (95% CI 0.89–0.92) and 0.93 (95% CI 0.92–0.94). The area under the curve (AUC) was 0.9806. The sensitivity and specificity results of Xpert GBS LB were 0.96 (95% CI 0.95–0.98) and 0.94 (95% CI 0.92–0.95), respectively. The AUC was 0.9950. No publication bias was found.

Conclusions
The Xpert GBS and GBS LB assays are valuable alternative methods with high sensitivity and specificity. However, determining whether they can be used as clinical diagnostic standards for GBS is essential for the future.
Comparison of therapy with β-lactam/β-lactamase inhibitor combinations or carbapenems for bacteraemia of nonurinary source caused by ESBL-producing Escherichia coli or Klebsiella pneumoniae
Authors: Luo Hong , Xiao Yanping , Hang Yaping , Chen Yanhui , Zhu Hongying , Fang Xueyao , Cao Xingwei , Zou Shan , Hu Xiaoyan , Xiong Jianqiu , Zhong Qiaoshi , Hu Longhua 
Published: September 2021

Abstract
Background
Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae has become a public health concern. This study aimed to compare the clinical outcomes of patients with nonurinary source bacteraemia caused by ESBL-producing Escherichia coli (E. coli) or Klebsiella pneumoniae (ESBL-producing EK) receiving β-lactam/β-lactamase inhibitor combinations (BLICs) versus carbapenem treatment and assess the risk factors of mortality with these two drugs.

Methods
We conducted a retrospective single-centre study of adult hospitalised patients with ESBL-producing EK bloodstream infection (BSI) from nonurinary source at our centre over a 4-year period. One hundred and eighty patients who received BLICs or carbapenems were included in the analysis. The outcome variables were 14-day treatment failure and 30-day mortality. For more reliable results, propensity score analysis was performed to compare the efficacy of the two drugs and analyse their risk factors for 30-day mortality.

Results
Out of 180 patients, 114 received BLICs, and 66 received carbapenem therapy. Compared to carbapenem-treated patients, those treated with BLICs were older and had higher age-adjusted Charlson comorbidity index, but they had shorter stay in the hospital. Additionally, their Pitt bacteraemia score, SOFA score, rate of leukaemia, and immune compromise were lower. After propensity score matching (PSM), the baseline characteristics of patients in the two treatment groups were balanced. BLICs were associated with a higher 14-day treatment failure rate (20.6%, 13/63) than carbapenems (16.3%, 7/43), although the difference was not significant in either univariate analysis (P = 0.429) or multivariate analysis (P = 0.122). And the 30-day mortality rate in BTG (11.1%, 7/63) and CTG (11.6%, 5/43) did not significantly differ (univariate analysis, P = 0.926; multivariate analysis, P = 0.420). In the multivariate analysis, after PSM, leukaemia was the only independent predictor of mortality in both BTG and CTG.

Conclusions
Our study showed that BLICs had higher 14-day treatment failure rate compared with carbapenems, although there were no statistically significant differences because of the small number of patients, therefore, further evaluation of the efficacy of BLICs is needed.
The role of dosimetry and biological effects in metastatic castration–resistant prostate cancer (mCRPC) patients treated with 223Ra: first in human study
Authors: Sciuto Rosa , Rea Sandra , Ungania Sara , Testa Antonella , Dini Valentina , Tabocchini Maria Antonella , Patrono Clarice , Soriani Antonella , Palma Valentina , Marconi Raffaella , Strigari Lidia 
Published: September 2021

Abstract
Background
223Ra is currently used for treatment of metastatic castration resistant prostate cancer patients (mCRPC) bone metastases with fixed standard activity. Individualized treatments, based on adsorbed dose (AD) in target and non-target tissue, are absolutely needed to optimize efficacy while reducing toxicity of α-emitter targeted therapy. This is a pilot first in human clinical trial aimed to correlate dosimetry, clinical response and biological side effects to personalize 223Ra treatment.

Methods
Out of 20 mCRPC patients who underwent standard 223Ra treatment and dosimetry, in a subset of 5 patients the AD to target and non-target tissues was correlated with clinical effects and radiation-induced chromosome damages. Before each 223Ra administrations, haematological parameters, PSA and ALP values were evaluated. Additional blood samples were obtained baseline (T0), at 7 days (T7), 30 days (T30) and 180 days (T180) to evaluate chromosome damage. After administration WB planar 223Ra images were obtained at 2–4 and 18–24 h. Treatment response and toxicity were monitored with clinical evaluation, bone scan, 18F-choline-PET/CT, PSA value and ALP while haematological parameters were evaluated weekly after 223Ra injection and 2 months after last cycle.

Results
1. a correlation between AD to target and clinical response was evidenced with threshold of 20 Gy as a cut-off to obtain tumor control; 2. the AD to red marrow was lower than 2 Gy in all the patients with no apparently correlation between dosimetry and clinical toxicity. 3. a high dose dependent increase of the number of dicentrics and micronuclei during the course of 223Ra therapy was observed and a linear correlation has been found between blood AD (BAD) and number of dicentrics.

Conclusions
This study provides some interesting preliminary evidence to be further investigated: dosimetry may be useful to identify a more appropriate 223Ra administered activity predicting AD to target tissue; a dose dependent complex chromosome damage occurs during 223Ra administration and this injury is more evident in heavily pre-treated patients; dosimetry could be used for radioprotection purpose.

Trial registration
The pilot study has been approved from the Ethics Committee of Regina Elena National Cancer Institute (N:RS1083/18–2111).
Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma
Authors: Kang Hyunkoo , Lee Sungmin , Kim Kyeongmin , Jeon Jaewan , Kang Seok-Gu , Youn HyeSook , Kim Hae Yu , Youn BuHyun 
Published: September 2021

Abstract
Background
Glioblastoma Multiforme (GBM) is a malignant primary brain tumor in which the standard treatment, ionizing radiation (IR), achieves a median survival of about 15 months. GBM harbors glioblastoma stem-like cells (GSCs), which play a crucial role in therapeutic resistance and recurrence.

Methods
Patient-derived GSCs, GBM cell lines, intracranial GBM xenografts, and GBM sections were used to measure mRNA and protein expression and determine the related molecular mechanisms by qRT-PCR, immunoblot, immunoprecipitation, immunofluorescence, OCR, ECAR, live-cell imaging, and immunohistochemistry. Orthotopic GBM xenograft models were applied to investigate tumor inhibitory effects of glimepiride combined with radiotherapy.

Results
We report that GSCs that survive standard treatment radiation upregulate Speedy/RINGO cell cycle regulator family member A (Spy1) and downregulate CAP-Gly domain containing linker protein 3 (CLIP3, also known as CLIPR-59). We discovered that Spy1 activation and CLIP3 inhibition coordinately shift GBM cell glucose metabolism to favor glycolysis via two cellular processes: transcriptional regulation of CLIP3 and facilitating Glucose transporter 3 (GLUT3) trafficking to cellular membranes in GBM cells. Importantly, in combination with IR, glimepiride, an FDA-approved medication used to treat type 2 diabetes mellitus, disrupts GSCs maintenance and suppresses glycolytic activity by restoring CLIP3 function. In addition, combining radiotherapy and glimepiride significantly reduced GBM growth and improved survival in a GBM orthotopic xenograft mouse model.

Conclusions
Our data suggest that radioresistant GBM cells exhibit enhanced stemness and glycolytic activity mediated by the Spy1-CLIP3 axis. Thus, glimepiride could be an attractive strategy for overcoming radioresistance and recurrence by rescuing CLIP3 expression.
A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia
Authors: Shumuye Nigus Abebe , Ohiolei John Asekhaen , Gebremedhin Mebrahtu Berhe , Yan Hong-Bin , Li Li , Li Wen-Hui , Zhang Nian-Zhang , Fu Bao-Quan , Jia Wan-Zhong 
Published: September 2021

AbstractBackgroundTapeworm infections are among the tropical neglected parasitic diseases endemically occurring in Ethiopia. This systematic review and meta-analysis aims at estimating the pooled prevalence and distribution ofTaeniaandEchinococcusinfections in humans and animals from reports from Ethiopia.MethodsThe systematic search was conducted in four bibliographic databases (PubMed, Google Scholar, Africa Journal Online and Science Direct). Additional data were retrieved from grey literature. Studies that met the inclusion criteria were considered for the systematic review and meta-analysis. The meta-analysis was conducted using MetaXL add-in for Microsoft Excel. Heterogeneity and inconsistency were evaluated using Cochran’sQandI2statistics, respectively.ResultsThe study provides a country-based database ofTaeniaandEchinococcusinfections consisting of 311 datasets from 201 publications which were mostly abattoir surveys; of these, 251 datasets were subjected to meta-analysis. Most of the studies were from Oromia (32.8%) followed by Amhara (22.9%) regional states. The pooled prevalence of cystic echinococcosis in intermediate and accidental hosts was calculated as 22% (95% CI 18–26%) and high study variability (Q = 24,420.65,I2 = 100%,P = 0.000). Moreover, a pooled prevalence ofEchinococcusinfections in final hosts was calculated as 33% (95% CI 20–48%) and low study variability (Q = 17.24,I2 = 65%,P = 0.001). Similarly, study subjects (human, cattle, sheep, goat and wolf) were infected byTaeniaspp. with pooled prevalence of 3% (95% CI 2–4%) and moderate study variability (Q = 279.07,I2 = 89,P = 0.000). Meanwhile, the pooled prevalence ofTaenia hydatigena,T. ovisandT. multicepsinfections in intermediate hosts were calculated as 38%, 14% and 5%, respectively. The random effect meta-analysis of bovine cysticercosis showed a pooled prevalence of 7% (95% CI 5–9%) and high study variability was of (Q = 4458.76;I2 = 99%,P = 0.000). Significant differences in prevalence ofTaeniaandEchinococcusinfections between study sites or different livestock origins have been reported.ConclusionThe study evidenced a comprehensive dataset on the prevalence and distribution ofTaeniaandEchinococcusinfections at different interfaces by regions and hosts and hence can aid in the design of more effective control strategies.Graphical Abstract
The pan-PPAR agonist lanifibranor reduces development of lung fibrosis and attenuates cardiorespiratory manifestations in a transgenic mouse model of systemic sclerosis
Authors: Derrett-Smith Emma , Clark Kristina E. N. , Shiwen Xu , Abraham David J. , Hoyles Rachel K. , Lacombe Olivier , Broqua Pierre , Junien Jean Louis , Konstantinova Irena , Ong Voon H. , Denton Christopher P. 
Published: September 2021

Abstract
Background
The TβRII∆k-fib transgenic (TG) mouse model of scleroderma replicates key fibrotic and vasculopathic complications of systemic sclerosis through fibroblast-directed upregulation of TGFβ signalling. We have examined peroxisome proliferator-activated receptor (PPAR) pathway perturbation in this model and explored the impact of the pan-PPAR agonist lanifibranor on the cardiorespiratory phenotype.

Methods
PPAR pathway gene and protein expression differences from TG and WT sex-matched littermate mice were determined at baseline and following administration of one of two doses of lanifibranor (30 mg/kg or 100 mg/kg) or vehicle administered by daily oral gavage up to 4 weeks. The prevention of bleomycin-induced lung fibrosis and SU5416-induced pulmonary hypertension by lanifibranor was explored.

Results
Gene expression data were consistent with the downregulation of the PPAR pathway in the TβRII∆k-fib mouse model. TG mice treated with high-dose lanifibranor demonstrated significant protection from lung fibrosis after bleomycin and from right ventricular hypertrophy following induction of pulmonary hypertension by SU5416, despite no significant change in right ventricular systolic pressure.

Conclusions
In the TβRII∆k-fib mouse strain, treatment with 100 mg/kg lanifibranor reduces the development of lung fibrosis and right ventricular hypertrophy induced by bleomycin or SU5416, respectively. Reduced PPAR activity may contribute to the exaggerated fibroproliferative response to tissue injury in this transgenic model of scleroderma and its pulmonary complications.
Chronic wasting disease: a cervid prion infection looming to spillover
Authors: Otero Alicia , Velásquez Camilo Duque , Aiken Judd , McKenzie Debbie 
Published: September 2021

AbstractThe spread of chronic wasting disease (CWD) during the last six decades has resulted in cervid populations of North America where CWD has become enzootic. This insidious disease has also been reported in wild and captive cervids from other continents, threatening ecosystems, livestock and public health. These CWD “hot zones” are particularly complex given the interplay between cervid PRNP genetics, the infection biology, the strain diversity of infectious prions and the long-term environmental persistence of infectivity, which hinder eradication efforts. Here, we review different aspects of CWD including transmission mechanisms, pathogenesis, epidemiology and assessment of interspecies infection. Further understanding of these aspects could help identify “control points” that could help reduce exposure for humans and livestock and decrease CWD spread between cervids.
RETRACTED ARTICLE: Implementation of MapReduce parallel computing framework based on multi-data fusion sensors and GPU cluster
Authors: Chang Dajun , Li Li , Chang Ying , Qiao Zhangquan 
Published: September 2021

AbstractNowadays, with the rapid growth of data volume, massive data has become one of the factors that plague the development of enterprises. How to effectively process data and reduce the concurrency pressure of data access has become the driving force for the continuous development of big data solutions. This article mainly studies the MapReduce parallel computing framework based on multiple data fusion sensors and GPU clusters. This experimental environment uses a Hadoop fully distributed cluster environment, and the entire programming of the single-source shortest path algorithm based on MapReduce is implemented in Java language. 8 ordinary physical machines are used to build a fully distributed cluster, and the configuration environment of each node is basically the same. The MapReduce framework divides the request job into several mapping tasks and assigns them to different computing nodes. After the mapping process, a certain intermediate file that is consistent with the final file format is generated. At this time, the system will generate several reduction tasks and distribute these files to different cluster nodes for execution. This experiment will verify the changes in the running time of the PSON algorithm when the size of the test data set gradually increases while keeping the hardware level and software configuration of the Hadoop platform unchanged. When the number of computing nodes increases from 2 to 4, the running time is significantly reduced. When the number of computing nodes continues to increase, the reduction in running time will become less and less significant. The results show that NESTOR can complete the basic workflow of MapReduce, and simplifies the process of user development of GPU positive tree order, which has a significant speedup for applications with large amounts of calculations.
Designated server-aided revocable identity-based keyword search on lattice
Authors: Guo Ying , Meng Fei , Cheng Leixiao , Dong Xiaolei , Cao Zhenfu 
Published: September 2021

AbstractPublic key encryption scheme with keyword search is a promising technique supporting search on encrypted data without leaking any information about the keyword. In real applications, it’s critical to find an effective revocation method to revoke users in multi-user cryptosystems, when user’s secret keys are exposed. In this paper, we propose the first designated server-aided revocable identity-based encryption scheme with keyword search (dSR-IBKS) from lattice. The dSR-IBKS model requires each user to keep just one private key corresponding with his identity and does not need to communicate with the key generation center or the server during key updating. We have proved that our scheme can achieve chosen keyword indistinguishability in the standard model. In particular, our scheme can designate a unique tester to test and return the search results, therefore no other entity can guess the keyword embedded in the ciphertext by generating search queries and doing the test by itself. We provide a formal security proof of our scheme assuming the hardness of the learning with errors problem on the standard model.