bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZBTB38 in Porcine Alveolar Macrophages Positively Regulates the Proliferation of Japanese Encephalitis Virus
Authors: Zheng Yi , Zhou Yu-Yong , Chai Chun-Xia , Cao San-Jie , Yan Qi-Gui , Huang Xiao-Bo , Wen Yi-Ping , Zhao Qin , Du Sen-Yan , Wu Rui 
Published: September 2021

Abstract
Background Japanese encephalitis (JE) is an important zoonotic disease caused by Japanese encephalitis virus (JEV), and pigs are intermediate host of this disease. Previous studies have confirmed that JEV can proliferate in the respiratory tract of mice and spread through it. Therefore, this study aimed to screen the proteins interacting with JEV on porcine alveolar macrophage cell and verify its role in the proliferation of JEV.Methods and results Porcine alveolar macrophages cell line 3D4/21 were infected with JEV, and obvious cytopathic effect (CPE) was observed. Zinc finger and BTB domain containing 38 (ZBTB38) was screened out as an interacting protein using co-immunoprecipitation assay and validated through knockout and overexpression of ZBTB38 in 3D4/21 cells. The results demonstrated that loss of ZBTB38 function basically had no effect on the attachment and entry processes of JEV, while the transcription level of JEV envelope gene, the expression level of NS3 protein and the number of virions were all significantly down-regulated in the subsequent infection stage. Conclusion Overall, one core conclusion was drawn in this paper that ZBTB38 promotes the proliferation of JEV especially in the middle and late stages of infection. This study provides new information for understanding the pathogenic mechanism of JEV, especially the respiratory transmission caused by JEV infection.
Dissolving Pulp From Eucalyptus Sawdust For Regenerated Cellulose Products
Authors: Olmos Graciela Viviana , Taleb María Claudia , Felissia Fernando Esteban , Ehman Nanci Vanesa , Peresin Maria Soledad , Area Maria Cristina , Vallejos Maria Evangelina , Maximino Mirtha Graciela 
Published: September 2021

Abstract
This study assesses the possibility of obtaining regenerated cellulose products (beads and films) from eucalyptus sawdust dissolving pulps produced by non-conventional processes, compared with a commercial dissolving pulp as a reference. Eucalyptus sawdust dissolving pulps were obtained by soda pulping followed by two different TCF sequential bleaching processes OOpZ and OOp (where O is oxygen, Op is oxygen reinforced with hydrogen peroxide, and Z is ozone), followed by a cold soda extraction. The characterization of dissolving pulps involved alpha-, beta- and gamma-cellulose content, alkali solubility with 10 wt% (S10), and 18 wt% NaOH (S18) aqueous solutions, and degree of polymerization. Fock´s method was used to measure cellulose reactivity and the alkali solubility in a 9 wt% NaOH aqueous solution at -5 °C to evaluate the pulps dissolving capacity. Dissolving pulps presented high cellulose content (> 93 %, expressed as a-cellulose) and good reactivity (almost 84 %). The dissolving pulps were adequate raw materials for regenerated cellulose products (beads and films) from two cellulose dissolution methods: direct dissolution in NaOH/urea and cellulose carbamate solution. The sequence OOpE (where E is an alkaline extraction) was determined to be a more economically feasible and straightforward process to produce dissolving pulp than OOpZE. The experimental pulps showed the expected characteristics of the dissolving pulp to obtain regenerated cellulose products. However, it is necessary to deepen the study of producing regenerated cellulose films with enhanced mechanical properties from experimental dissolving pulps, solvents, coagulation, and regeneration conditions.
Identification and Validation of Novel Biomarkers for HBV-Associated Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis: A Case Control Study
Authors: Zhao Dandan , Zhang Xiaoxiao , Tang Yuhui , Guo Peilin , Ai Rong , Hou Mengmeng , Wang Yiqi , Yuan Xiwei , Cui Luyao , Zhang Yuguo , Zhao Suxian , Li Wencong , Wang Yang , Sun Xiaoye , Liu Lingdi , Dong Shiming , Li Lu , Zhao Wen , Nan Yuemin 
Published: September 2021

Abstract
BackgroundAt present, there are no accurate diagnostic biomarkers for distinguishing among hepatitis B virus (HBV)-related liver diseases. This research aimed to identify and validate a novel biomarker panel in patients with HBV-related hepatocellular carcinoma (HCC), liver fibrosis/liver cirrhosis (LF/LC) and chronic hepatitis B (CHB).MethodsTranscriptomics sequencing was conducted on a total of 19 liver tissues of the four groups. The hub mRNAs were identified using weighted gene co-expression network analysis (WGCNA) and short time-series expression miner (STEM) method. The expression levels of selected mRNAs and proteins were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) using both liver tissues and peripheral blood mononuclear cells (PBMCs) and immunohistochemical (IHC) staining in liver tissues, respectively. Then, the expression activities of the screened targets were detected by enzyme-linked immunosorbent assay (ELISA) using the plasma samples from 200 and 400 subjects in the validation and testing sets, respectively. Finally, receiver operating characteristic (ROC) curve analysis was carried out to evaluate the diagnostic performance of each mRNA biomarker.ResultsSTEM analysis revealed 25 mRNAs exhibited an increasing trend in the four groups; while 9 hub mRNAs were identified by using WGCNA. Of note, the results of qRT-PCR and immunohistochemical analyses demonstrated that SHC adaptor protein 1 (SHC1), SLAM family member 8 (SLAMF8), and interleukin-32 (IL-32) exhibited a gradually increasing trend in the four groups. Subsequent ELISA tests on the validation cohort demonstrated that the plasma levels of SHC1, SLAMF8 and IL-32 were remarkably elevated in HCC group compared to the remaining three groups. Furthermore, a diagnostic model APFSSI (age, PLT, ferritin, SHC1, SLAMF8 and IL-32) was established to distinguish HCC from LF/LC (AUC=0.904), LF/LC from CHB (AUC=0.924), and CHB from healthy controls (AUC=0.966). Finally, the results in the test set were consistent with those in the validation set. ConclusionsSHC1, SLAMF8 and IL-32 can differentiate among HCC patients, LF/LC patients, CHB patients and healthy controls. More importantly, the combination of age, PLT, ferritin, SHC1, SLAMF8, and IL-32 (APFSSI model) greater improves the diagnostic accuracy of HBV-associated liver diseases.
Prevalence of Selected Cardiometabolic Risk Factors in The Global ART-Naïve HIV Infected Population: A Protocol for a Systematic Review and Meta-Analysis
Authors: Ebasone Peter Vanes , Peer Nasheeta , Dzudie Anastase , Kengne Andre Pascal 
Published: September 2021

Abstract
Background
People living with HIV/AIDS (PLHIV) are at increased risk of cardiometabolic diseases attributable to the effects of the virus, antiretroviral therapy (ART) and traditional risk factors found in the general population. Most studies have focused on assessing the effect of ART on cardiometabolic disease in PLHIV with fewer studies assessing the cardiometabolic risk profile prior to any exposure to ART. Therefore, this protocol is for a systematic review and meta-analysis to estimate the global prevalence of selected cardiometabolic risk factors in ART-naïve PLHIV and their association with HIV specific factors.
Methods
We shall conduct a systematic search of published literature for observational studies on the prevalence of obesity, hypertension, diabetes and dyslipidaemia (high Low-Density Lipoprotein Cholesterol, high Total Cholesterol, high Triglyceride, low High-Density Lipoprotein Cholesterol) in ART-naïve PLHIV and their association with HIV specific characteristics. We will search PubMed-MEDLINE, CINAHL, SCOPUS, Academic Search Premier, Africa-Wide Information and Africa Journals Online databases to identify relevant studies published before March 2021. Two authors will independently screen, select studies, extract data and conduct risk of bias assessments. Disagreements between the two authors will be resolved by consensus or consulting a third reviewer. Data consistently reported across studies will be pooled using random-effects meta-analysis. Heterogeneity will be evaluated using Cochrane’s Q statistic and quantified using I2 statistics. The Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines are used for the reporting of this systematic review protocol.
Discussion
This review will help determine the burden of selected cardiometabolic diseases in ART-naïve HIV-infected populations and the contribution of HIV infection, independent of ART, to cardiometabolic diseases in PLHIV. It will provide new information that can help orientate future research and potentially guide healthcare policy making. This is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine with protocol ethical clearance number (UCT HREC 350/2021).
Registration
PROSPERO: CRD42021226001
Neuropsychological Outcomes After Awake Resections of Right Frontal IDH-Mutated Glioma: Insights From a Consecutive Series of 20 Cases.
Authors: Barberis Marion , Poisson Isabelle , Facque Valentine , Letrange Sophie , Prévost-Tarabon Cécile , Houdart Emmanuel , Froelich Sébastien , Levy Richard , MANDONNET Emmanuel 
Published: September 2021

Abstract
Background

Awake surgery for low-grade gliomas is currently considered the best procedure to improve the extent of resection and guarantee a "worth living life" for patients, meaning avoiding not only motor but also cognitive deficits. However, tumors located in the right hemisphere, especially in the right frontal lobe, are still rarely operated on in awake condition; one of the reasons possibly being that there is little information in the literature describing the rates and nature of long-lasting neuropsychological deficits following resection of right frontal glioma.
Objective

To investigate long-term cognitive deficits after awake surgery in right frontal IDH-mutated glioma.
Methods

We retrospectively analyzed a consecutive series of awake surgical resections between 2012 and 2020 for right frontal IDH-mutated glioma. We studied the patients' subjective complaints and objective neuropsychological evaluations, both before and after surgery. Our results were then put in perspective with the literature.
Results

Twenty surgical cases (including 5 cases of redo surgery) in eighteen patients (medium age: 42.5 [range 26–58]) were included in the study. The median preoperative volume was 37 cc; WHO grading was II, III and IV in 70%, 20%, and 10% of cases, respectively. Preoperatively, few patients had related subjective cognitive or behavioral impairment, while evaluations revealed mild deficits in 45% of cases, most often concerning executive functions, attention, working memory and speed processing. Immediate postoperative evaluations showed severe dysexecutive syndrome in 75% of cases but also attentional deficits (65%), spatial neglect (60%) and behavioral disturbances (apathy, aprosodia/amimia, emotional sensitivity, anosognosia). Four months after surgery, although psychometric z-scores were unchanged at the group level, individual evaluations showed a slight decrease in performance in 9/20 cases (dysexecutive syndrome, speed processing, attention, semantic cognition, social cognition).
Conclusion

Our results are generally consistent with those of the literature, confirming that the right frontal lobe is a highly eloquent area and highlighting the importance of operating these patients in awake conditions.
The Effects of Subthreshold Autistic Traits On Time Perception: The Regulation of Interpersonal Information Association
Authors: Xuan Bin , Li Shuo , Yang Lu 
Published: September 2021

Abstract
Background

People with high subthreshold autistic traits usually show structural impairments like those with autism spectrum disorder, but with less social and cognitive impairments. The effect of autistic traits on time perception and the role of interpersonal information on this effect remains unexplored.
Methods

This study used a temporal bisection task between 400–1600 ms to compare the time perception of individuals with higher and lower autistic traits, and to explore the influence of interpersonal information on their time perception by establishing associations between interpersonal information and geometric figures. A total of 32 participants with high autistic traits and 31 participants with low autistic traits took part in the study.
Results

In the absence of identity information, people with high autistic traits tended to judge short durations as longer, and their Weber ratio was higher than those with low autistic traits, suggesting that their overestimation of short duration is due to decreased temporal sensitivity and increased internal clock frequency. With the addition of interpersonal information, individuals with high and low autistic traits had faster responses to self in the identity-figure association, and the subjective bisection point was shorter. However, it took longer for individuals with high autistic traits to build the self-association, and there was no difference in the proportion of long response and Weber ratio between individuals with high and low autistic traits when identity was involved.
Conclusion

These results suggest that individuals with high autistic traits have a higher central tendency, and this change is related to the decline of perceptual sensitivity. Actively guiding attention to interpersonal information can improve the time perception sensitivity of individuals with high autistic traits.
Submitted as a Research Report to Biology of Sex Differences
Authors: Grimm Jeffrey , North Katherine , Hopkins Madeleine , Jiganti Kyle , McCoy Alex , Šulc Josef , MacDougall Derek , Sauter Frances 
Published: September 2021

Abstract
Background: There are sex differences in addiction behaviors. To develop a pre-clinical animal model to investigate this, the present study examined sex differences in sucrose taking and seeking using LongEvans rats. Methods: Five experiments were conducted using separate groups of subjects. The first two examined sucrose or saccharin preference in two-bottle home cage choice tests. Experiment three assessed sucrose intake in a binge model with sucrose available in home cage bottles. Experiments four and five utilized operant-based procedures. In Experiment four rats responded for sucrose on fixed and progressive ratio (FR, PR) schedules of reinforcement over a range of concentrations of sucrose. A final component of experiment four was measuring seeking in the absence of sucrose challenged with the dopamine D1 receptor antagonist SCH23390. Experiment five assessed responding for water on FR and PR schedules of reinforcement. Results: When accounting for body weight, female rats consumed more sucrose than water; but there was no sex difference in saccharin preference over a range of saccharin concentrations. When accounting for body weight, females consumed more sucrose than males in the binge model, and only females increased binge intake over the 14 days of the study. Females responded at higher rates for sucrose under both FR and PR schedules of reinforcement. Females responded at higher rates in extinction (seeking); SCH23390 reduced sucrose seeking of both females and males. Females responded at higher rates for water on FR and PR schedules than males, although rates of responding were low and decreased over sessions. Conclusions: Across bottle-choice, binge intake, and operant procedures, female Long-Evans rats consumed more sucrose and responded at higher rates for sucrose. Although females also responded more for water, the vigor of responding did not explain the consistent sex difference in sucrose taking and seeking. The sex difference in sucrose taking was also not explained by sweet preference, as there was no sex difference in saccharin preference. These data corroborate with findings of sex differences in addiction behaviors in humans, providing a pre-clinical model to further evaluate sex differences in these behaviors and manipulations designed to reduce them.
Determinants of Intention To Use Maternity Waiting Homes in Tanzania: A Study Based on Women Using MWH in Dodoma, Iringa and Morogoro
Authors: Luvanda Habiel Benjamin , Mbogoro Erick Justin 
Published: September 2021

Abstract
Background
Maternal mortality rate (MMR) in Tanzania is still persistently high. The data from Tanzania has revealed that maternal mortality ratio (MMR) has increased from 454 per 100,000 live births in 2010 to 556 per 100,000 live births by the year 2015. Establishment of Maternity Waiting Homes (MWH’s) in a few regions has been one of the efforts for trying to serve lives of pregnant women from pregnancy related complication.
Methods
The study adopted a cross-sectional study design and a convenience sampling technique for recruiting the respondents.
Results
The women aged 20–34 are 10 times more likely to use the MWHs in case of future pregnancy (OR = 10.906, p = 0.019) whereas those aged 35 to 49 are 54 times more likely to use MWHs in case of future pregnancy (OR = 54.629, p = 0.006) as compared to those aged 18 to 24 years old. Women who lived 3 to 6 hours of travel to MWH are 11 times more likely to use MWH in case of future pregnancy as compared to those living at the distance of 1 to 3 hours to the MWH (OR = 11.451, p = 0.032).
Conclusion
The results of this study have revealed some interesting findings in the issues of distance by traveling time and age of the respondent to be highly significant factors in predicting intention to use MWHs in case of future pregnancy.
SN 2018bsz: significant dust formation in a nearby superluminous supernova
Authors: Chen Ting-Wan , Brennan Seán , Wesson Roger , Fraser Morgan , Schweyer Tassilo , Inserra Cosimo , Schulze Steve , Nicholl Matt , Anderson Joseph , Hsiao Eric , Jerkstrand Anders , Kankare Erkki , Kool Erik , Kravtsov Timo , Kuncarayakti Hanindyo , Leloudas Giorgos , Li Chuan-Jui , Matsuura Mikako , Pursiainen M. , Roy Rupak , Ruiter Ashley , Schady Patricia , Seitenzahl Ivo , Sollerman Jesper , Tartaglia Leonardo , Wang Lifan , Yates Rob , Yang Sheng , Baade Dietrich , Carini Roberta , Gal-Yam Avishay , Galbany Lluís , González-Gaitán Santiago , Gromadzki Mariusz , Gutierrez Claudia , Kotak Rubina , Maguire Kate , Mazzali P P.A. , Müller-Bravo Tomas , Paraskeva Emmy , Pessi Priscila , Pignata Giuliano , Rau Arne , Young David 
Published: September 2021

Abstract
We investigate the thermal emission and extinction from dust associated with the nearby superluminous supernova (SLSN) 2018bsz. Our dataset has daily cadence and simultaneous optical and near-infrared coverage up to ~ 100 days, together with late time (+1.7 yr) MIR observations. At 230 days after light curve peak the SN is not detected in the optical, but shows a surprisingly strong near-infrared excess, with r - J > 3 mag and r - Ks > 5 mag. The time evolution of the infrared light curve enables us to investigate if the mid-infrared emission is from newly formed dust inside the SN ejecta, from a pre-existing circumstellar envelope, or interstellar material heated by the radiation from the SN. We find the latter two scenarios can be ruled out, and a scenario where new dust is forming in the SN ejecta at epochs > 200 days can self-consistently reproduce the evolution of the SN flux. We can fit the spectral energy distribution well at +230d with 5x10-4 solar mass of carbon dust, increasing over the following several hundred days to 10-2 solar mass by +535d. SN 2018bsz is the first SLSN showing evidence for dust formation within the SN ejecta, and appears to form ten times more dust than normal core-collapse SNe at similar epochs. Together with their preference for low mass, low metallicity host galaxies, we suggest that SLSNe may be a significant contributor to dust formation in the early Universe.
Strategies for Persistent Intracranial Infection Associated With Subcutaneous Effusion in the Posterior Fossa
Authors: Cai Qing , Wang Shoujie , Zheng Min , Qin Huaizhou , Feng Dayun 
Published: September 2021

Abstract
Objective: Due to the particularity of anatomy, there are many subcutaneous effusions after posterior fossa surgery. This paper discusses the characteristics and treatment strategies of persistent infection related to subcutaneous effusions in the posterior fossa. Methods: Seventeen patients with persistent intracranial infection after neurosurgical posterior fossa surgery from March 2015 to July 2020 were retrospectively analyzed. According to different stages of infection, the treatment process of intracranial infection was divided into the acute infection stage, clinical response stage and infection cure stage, and the measures taken in the different stages were summarized.Results: Compared with the acute infection stage, the indices of body temperature, blood and cerebrospinal fluid in the clinical response stage were improved, but there was no significant difference. There was a significant difference in each index between the acute infection stage and the infection cure stage. After the infection was cured, 17 patients were significantly relieved or cured of subcutaneous effusions by various methods.Conclusion: It is necessary to be alert to the existence of subcutaneous effusions in cases of poor effects or repeated infections after routine treatment. Multiple replacements and flushing of subcutaneous effusions are an important means of treating this kind of infection.
Transition Interventions for Adolescents on Antiretroviral Therapy on Transfer From Pediatric to Adult Healthcare: A Systematic Review Protocol.
Authors: Jegede Olubukola Esther , Wyk Brian Van 
Published: September 2021

Abstract
Background: Adolescents living with HIV (ALHIV) experience poor health outcomes such as low retention in care, suboptimal adherence to ART, insufficient viral suppression, high morbidity and mortality. These outcomes coincide with the period during and after their transition from pediatric to adult healthcare. While some studies on transition interventions have reduced poor health outcomes among ALHIV, some have reported no significant reduction. There is a need to systematically describe, assess the quality and make recommendations regarding the effects of the transition interventions' compendium. The proposed study aims to systematically describe interventions that guide the transition of adolescents on ART from pediatric to adult care and synthesize evidence of the effects on adherence, retention in care and viral load suppression. Methods: A systematic review of interventions that guide the transition of adolescents on ART and report on treatment outcomes (adherence, retention in care and viral load suppression) will be conducted. Studies will be sourced from electronic databases (PubMed, Scopus, Web of Science, Ebscohost, CINAHL, Science Direct, Google scholar and the World Health Organization's (WHO's) library database). All studies will be exported to Mendeley, and two independent reviewers will screen the titles and abstracts and the full text according to the eligibility criteria. The risk of bias will be determined using Cochrane Collaboration tool RoB 2, the Newcastle–Ottawa Scale and the Risk of Bias in Non-randomized Studies - of Interventions checklists. The strength of evidence will be evaluated using the Grading of Recommendations Assessment, Development and Evaluation approach. A narrative synthesis including the description of characteristics of studies, comparison of differences and similarities between studies and description of possible trends will be carried out. If possible, homogenous studies will be subjected to a meta-analysis.Discussion: the review findings will provide a robust knowledge base on transition interventions available to ALHIV. Assessing the strength and quality of studies will ascertain the magnitude of the effect of transition intervention outcomes and inform the development of protocols and treatment guidelines needed to achieve better health outcomes for ALHIV. Systematic review registration: Submitted to PROSPERO on the 12th of August 2021