bluuebunny/crossref_metadata_2025_split huggingface dataset:
Umbilical Cord Mesenchymal Stem Cells-derived Exosomes Deliver Mir-21 to Accelerate Corneal Epithelial Wound Healing Through PTEN/PI3K/Akt pathway
Authors: Li Xuran , Liu Xiaolong , Zhang Yanyan , Liu Zhiyu , Li Xinyue , Yu Yu , Ye Xiangmei , Li Yang , Yang Dongguang , Teng Yueqiu , Shi Ce , Jin Xin , Qi Sen , Liu Yuting , Wang Shudan , Liu Ying , Cao Fenglin , Kong Qingran , Wang Zhenkun , Zhang Hong 
Published: June 2021

Abstract
BackgroundRapid restoration of corneal epithelium integrity after injury is particularly important for preserving corneal transparency and vision. Mesenchymal stem cells (MSCs) can be taken into account as the promising regenerative therapeutics for improvement of wound healing processes based on the variety of the effective components. The extracellular vesicles form MSCs, especially exosomes, has been considered as important paracrine mediators though transferring microRNAs into recipient cell. This study investigated the mechanism of human umbilical cord MSC-derived exosomes (HUMSC-exosomes) on corneal epithelial wound healing.MethodsExosomes extracted from HUMSCs were identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Corneal fluorescein staining and histological staining were evaluated in a corneal mechanical wound model. Changes in HCECs proliferation after HUMSC-exosomes or miR-21 mimic treatment were evaluated by CCK-8 and EdU assays, while migration was assessed by in vitro scratch wound assay. Full-length transcriptome sequencing was performed to identify the differentially expressed genes associated with HUMSC-exosomes treatment, followed by validation via real-time PCR and Western blot.ResultsThe exosomes derived from HUMSCs can significantly promote corneal epithelial cells proliferation, migration in vitro and accelerate corneal epithelial wound healing in vivo. Similar effects were obtained after miR-21 transfection, while the beneficial effects of HUMSC-exosomes were partially negated by miR-21 knockdown. Results also show that the benefits are associated with decreased PTEN level and activated the PI3K/Akt signaling pathway in HCECs.Conclusions﻿HUMSC-exosomes could accelerate the recovery of corneal epithelial wounds though restraining PTEN by transferring miR-21, and may represent a promising novel therapeutic agent for corneal wound repair.
Tetraspanin 3, as a Novel Regulator of Intracellular β1 Integrin Recycling, Promotes Non-Small Cell Lung Cancer Cell Proliferation
Authors: Su Hongbo , Xu Yitong , Zhang Yao , Jiang Xizi , Zhang Qingfu , Ren Hongjiu , Zou Zifang , Qiu Xueshan 
Published: June 2021

Abstract
Background: The role of tetraspanins in cancer development has been widely reported. However, the expression and roles of tetraspanin 3 (TSPAN3) in solid tumors, including non-small cell lung cancer (NSCLC), have not yet been extensively investigated. In this study, we explored the role of TSPAN3 in NSCLC and its potential role in the trafficking of integrin β1, which is highly expressed in this type of cancer.Methods: The expression of TSPAN3 in NSCLC tissue and NSCLC cell lines was evaluated, and the correlation between TSPAN3 expression and disease progression was assessed. Furthermore, loss- and gain-of-expression studies were conducted to determine the biological function of TSPAN3 both in vivo and vitro. The regulatory role of TSPAN3 in β1 integrin expression and intracellular recycling was studied through western blot, RT-PCR, and immunofluorescence analyses.Results: TSPAN3 was found to be highly expressed in lung cancer cells and tissues. Moreover, high levels of TSPAN3 positively correlated with poor differentiation, lymph node involvement, advanced pathological tumor-node-metastasis stage, and poor prognosis in NSCLC patients. TSPAN3 showed the potential to promote the proliferation of NSCLC cells in vitro and in vivo. Specifically, TSPAN3 was found to interact with β1 integrin and Rab11a, thereby facilitating the sorting of β1 integrin into Rab11a endosomes and promoting β1 integrin recycling and upregulation. Conclusions: These findings reveal a novel role for TSPAN3 in the regulation of intracellular recycling of β1 integrin. Hence, TSPAN3 may represent a potentially valuable therapeutic target for NSCLC.
The Impact of Big Data on Banking Operations
Authors: Hasan Morshadul , Le Thi , Hoque Ariful 
Published: June 2021

Abstract
In the current technological advances with the rise of the information revolution through mobile internet, cloud computing, big data, and the Internet of Things (IoT), the banking industry is receiving new opportunities and facing critical challenges. It motivates us to develop the proposed research concept to examine how data innovation influences banking operations. We employ the systematic qualitative research methodology based on the existing literature from Web of Science and SCOPUS database to accomplish our research objectives. The findings of this study include the positive implications of big data, challenges, and banking security as essential data-driven banking issues. This research will have a significant implication in the banking industry that big data operation is critical for data-driven banking decisions.
The Cadillac Risk Score Accurately Identifies Patients at Low Risk for In-Hospital Mortality and Adverse Cardiovascularevents Following St Elevation Myocardial Infarction.
Authors: Wilson Ryan S. , Malamas Peter , Dembo Brent , Lall Sumeet K , DO Ninad Zaman , Peterson Brandon R 
Published: June 2021

Abstract
Background: The CADILLAC risk score was developed to identify patients at low risk for adverse cardiovascular events following ST elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention (PPCI). Methods: We performed a single center retrospective review of STEMI hospitalizations treated with PPCI from 2014 to 2018. Patients were stratified using the CADILLAC risk score into low risk group versus intermediate to high risk group. Patients presenting with cardiac arrest or cardiogenic shock were excluded from the study. The primary outcome was adverse clinical events during initial hospitalization. Secondary outcomes were adverse clinical events at 30 days and 1 year following index hospitalization. Results: The study included 314 patients. It was found that patients with a low CADILLAC score had significantly lower adverse clinical events compared to the intermediate-high CADILLAC score group (10/213 (4.7%) vs. 15/128 (11.7%), odds ratio = 0.37, 95% CI 0.16-0.85, p= 0.028). Additionally, patients with a low CADILLAC score had significantly lower adverse clinical event rates at 30 day and 1 year follow up. The mortality rate was 0% for patients defined at low risk by CADILLAC score during hospitalization, as well up to 1 year follow up. ROC curve predicting in hospital event rate showed CADILLAC (C=0.66, odds ratio 1.18; 95% CI 1.04 - 1.33; p=0.0064). Conclusion: Patients defined as low risk by the CADILLAC score following a STEMI were associated with lower event rates when compared to those with an intermediate-high CADILLAC score.
Influence of Particle Distribution on Macroscopic Properties of Particle Flow Model
Authors: Gou Bin , Zhang Mengqi , Mo Jiliang 
Published: June 2021

Abstract
The particle flow discrete element models for uniaxial compression and tensile tests of rocks are established to study the influence of the particle distribution randomness on the macroscopic mechanical properties of such model. The results of macroscopic mechanical properties show strong discreteness due to the variance of particle distributions, in which compressive strength, tensile strength and Poisson's ratio follow the normal distribution and the Young's modulus follows the negative skewness distribution. The average values of the macroscopic strength obtained based on multiple calculations with different particle distributions should be used for the calibration of microscopic parameters. According to the relationship between sample size and deviation of macro strength averages, the minimum calculation number required to obtain high-precision macro strength with different confidence levels is given.
LncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived From ADSCs for The Treatment of Osteoporosis
Authors: Wang Shanzheng , Jia Jun , Chen Chang-hong 
Published: June 2021

Abstract
Background

Osteoporosis is a worldwide medical and socioeconomic threat characterized by systemic impairment to bone strength and microstructure. Exosomes derived from adipose-derived stem cells (ADSCs-Exos) have been confirmed to play effective roles in the repair of various tissues and organs. This study aimed to investigate the role of ADSCs-Exos and a noval long none coding RNAKCNQ1OT1 (lnc-KCNQ1OT1) played in osteoporosis as well as the underlying mechanism.
Methods

MC3T3-E1 cells were treated with different doses of TNF-α (0, 1, 2.5, 5, 10 ng/ml) and then co-cultured with ADSCs-Exos or exosomes-derived from lnc-KCNQ1OT1-modified ADSCs (KCNQ1OT1-Exos). The expression of microRNA (miRNA)-141-5p (miR-141-5p) and lnc-KCNQ1OT1 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression of cleaved-caspase-3, caspase-3 and Bax was determined by Western blot. Cell viability and apoptosis were assessed by cell counting kit 8 (CCK-8) and flow cytometry analysis, respectively. The binding sites between KCNQ1OT1 and miR-141-5p were validated by dual-luciferase reporter assay.
Results

Tumor necrosis factor-α (TNF-α) dose dependently increased miR-141-5p expression, inhibited viability and promoted apoptosis of MC3T3-E1 cells. However, miR-141-5p silencing or co-culture with ADSCs-Exos attenuated these effects. In addition, KCNQ1OT1-Exos could more significantly attenuate the induced cytotoxicity and apoptosis compared to ADSCs-Exos. Moreover, miR-141-5p was confirmed as the target of lnc-KCNQ1OT1 by luciferase reporter assay.
Conclusions

ADSCs-Exos attenuated cytotoxicity and apoptosis of TNF-α-induced MC3T3-E1 cells. KCNQ1OT1-Exos had a more significant inhibitory effect compared to ADSCs-Exos by the function of sponging miR-141-5p, suggesting that KCNQ1OT1-Exos could be promising agents in osteoporosis treatment.
Determinant of Early Age Sexual Initiation: Associated Factors Among Rural Women in Ethiopia: Evidence From EDHS 2016.
Authors: Mulugeta Solomon Sisay , Gebremichael Shewayiref Geremew 
Published: June 2021

Abstract
Background: The aim of this study was to examine the determinant factors on early age sexual intercourse among rural women in Ethiopia.Methods: This study contains further analysis of the Ethiopian Demographic and Health Survey (EDHS) 2016 dataset. A total of 8001 participants of under reproductive age (15–49 years) women, who had at least one set of sexual intercourse, were involved in the analysis. The binary logistic multivariable analysis result was obtained by STATA-14 software. Results: Among the rural women in Ethiopia, more than half (59.6%) of women was experienced sexual intercourse at early age. The higher proportion of experiencing early age sexual initiation among rural women was found in Amhara (18.63%) region. Age of women [(OR= 0.374;95%CI:0.301-0.465) of 20-24 years, (OR= 0.44;95%CI:0.36-0.54) of 25-49 years], geopolitical region [Amhara (OR=1.514; 95%CI:1.25–1.83), Oromia (OR=0.74; 95%CI:0.64-0.905), Somali(OR=0.47; 95% CI: 0.364-0.658), SNNPR (OR= 0.5; 95% CI: 1.13–1.81), and Dire Dawa (OR=0.68; 95% CI: 0.49-0.941)]; women age at first birth (OR=0.3; 95% CI: 0.11-0.5) of greater than 18 years old; HIV/AIDS awareness (OR=0.4; 95% CI: 0.183-1.604, had awareness); chat chewing(OR=1.23; 95% CI:1.03-1.47); willingness at first sex (OR=2.51; 95% CI: 1.22-3.8, by force); marital status [married women (OR=2.52; 95% CI:1.69-3.75); divorced/separated women(OR=2.39; 95% CI: 1.58-3.63)]; and educational attainment [secondary school and above completed (OR= 0.231; 95% CI: 0.183-0.291) and primary level of education (OR= 0.67; 95% CI: 0.06-0.76)] were examined as statistically significant factors.Conclusion: The commencement of early age sexual initiation among reproductive-age women was high. This study suggests that continued programmatic and policy initiatives should be directed to improve adolescent’s overall situation as a means of improving their sexual health.
Uniportal Versus Needlescopic Video-Assisted Thoracoscopic Surgery for Primary Spontaneous Pneumothorax: A Comparable Study
Authors: Chuang Jen-Hao , Hsu Hsao-Hsun , Lin Mong-Wei , Huang Pei-Ming , Kuo Shuenn-Wen , Chen Ke-Cheng , Chen Jin-Shing 
Published: June 2021

Abstract
Objectives:Primary spontaneous pneumothorax (PSP) is a common disease in young and thin male. Operation has been regarded as definitive treatment for it. However, the operative methods for those patients are under dispute. Our study is aimed to compare the uniportal VATS versus needlescopic VATS for PSP patients in our institute.Methods:From July 2013 to December 2017, the patients who underwent video-assisted thoracic surgery for pneumothorax in National Taiwan University Hospital were retrospectively collected. The preoperative condition, surgical results, and postoperative outcomes was analyzed.Results:There were 60 patients undergoing needlescopic VATS and 91 undergoing uniportal VATS during the study period. There was no significant difference between the patients who underwent needlescopic VATS and those who underwent uniportal VATS in their demorgraphic and clinical characteristics. The post-operative pain score was significantly lower in the uniportal VATS group compared to the mini VATS group at day 1 (2.65 ± 1.59 vs. 1.74 ± 1.35, p = 0.001).Conclusion:We have shown that uniportal VATS is an effective and a safe treatment for PSP patients, and it is comparable to needlescopic VATS. Based on these results, we believe that uniportal VATS using a wound protector can be used as an alternative approach for PSP.
Bacterial Predation on T4 Phages
Authors: godon jean-jacques , bize ariane , ngo hoang , cauquil laurent , almeida mathieu , petit marie-agnes , zemb olivier nicolas 
Published: June 2021

Abstract
Background: Bacterial consumption of viruses has never yet been reported, even though bacteria feed on almost anything. Viruses are omnipresent predators for all organisms, but have no acknowledged active biocontrol. The viral biomass undoubtedly reintegrates the trophic cycles, however the mechanisms of this phase still remain unknown.Methods: Here, we used stable isotope probing with 13C labelled T4 phages to monitor the increase of density of the bacterial DNA concomitant with the decrease of plaque forming units. We used 12C T4 phages as control.Results: T4 phage disappearance in wastewater sludge was found to occur mainly through predation by Aeromonadacea. Phage consumption also favours significant in situ bacterial growth. Furthermore, an isolated strain of Aeromonas was observed to grow on T4 phages as sole source of carbon, nitrogen and phosphorus.Conclusions: bacterial species are capable of consuming bacteriophages in situ, which is likely a widespread and underestimated type of biocontrol. This assay is anticipated as a starting point for harnessing the bacterial potential in limiting the diffusion of harmful viruses within environments such as gut or water.
Health-related Quality of Life Among People Who Inject Drugs on Methadone Assisted Treatment in Zanzibar, Tanzania
Authors: Haji Shaaban Hassan , Damian Damian Jeremia , Rajab Nahir Ali , Ali Othman Mkubwa , Khamis Asha Ussi , Khalid Farhat Jowhar , Sunguya Bruno Fokas 
Published: June 2021

Abstract
Background: The burden of drug use is growing rapidly in Low- and Middle-Income countries (LMICs) with access to coastlines and most major cities;Tanzania is not exception. Methadone Assisted Treatment (MAT) has a potential to reduce addiction and therefore health and quality of lives. MAT is known to improve health related quality of life and it is essential in addressing a number of drug use related challenges among People Who Inject Drugs (PWIDS). Evidence on the profiles of Health Related Quality of Life (HRQOL) among PWIDS who are in treatment programmes particualry MAT services, is scanty in Zanzibar, Tanzania, Objectives: To determine HRQOL and associated factors among PWIDS receiving MAT Services in Zanzibar, Tanzania. Methods: This was a cross-sectional study conducted in Unguja, Zanzibar from July to August 2019. A total of 376 PWIDS attending Kidongo Chekundu MAT clinic were randomly selected and enrolled in the study. Data were electronically collected using Open Data Kit (ODK) programme during face-to-face interviews with PWIDS HRQOL was measured using the WHO's Quality of Life questionnaire Descriptive and binary logistic regression analyses were used to describe and determine factors independently associated with HRQOL. Results: Of the 376 PWIDS enrolled in this study, Vast majority (92.6%) were male. The mean age of participants at enrollment was 39 (± standard deviation (SD) 8.9). Slightly more than one third (45.5%) were single, 56.4% have a secondary school education level. Slightly more than two third (82.7%) were employed, 42% reported living at Magharibi B districts. High proportion were on MAT services for more than one year (70.2%); had good adherence to MAT services (79%), and were receving 50 – 99 mg of methadodone dose per day (51.1%).The majority of PWIDS (82%) had of high HRQOL. Employed PWIDS [AOR=5.36; 95%CI (1.51, 19.04); p=0.009], and PWIDS who were in the middle wealth quintile [OR=24.15; 95%CI (2.01, 290.30); p=0.012] were more likely to have better HRQOL. Conclusion: In general, PWIDS on MAT services have good HRQOL in Zanzibar. Being employed or having better economic status among were associated with better HRQOL. To effect this there is a need to strengthen multisectoral collaborations, integrated and comprehensive MAT services within the clinic and other related areas with all relevant stakeholders so as to improve health related quality of life among PWIDS.
Effect of targeted fluid minimization and diuretic therapy on hospital- acquired heart failure and adverse outcomes in patients with no preexisting cardiac dysfunction
Authors: Intarawongchot Kwannate , Gonlachanvit Sutep , Puwanant Sarinya 
Published: June 2021

Abstract
Background This study aimed to investigate: 1) the incidence of hospital-acquired heart failure (HF) in patients with no preexisting cardiac disorder, and 2) whether the use of an intervention protocol comprised of targeted fluid minimization and diuretic therapy can reduce the incidence of hospital-acquired HF and adverse outcomes.Methods We conducted a single-center, open-labeled, prospective cohort study enrolling patients with no preexisting cardiac dysfunction who were admitted to the medical wards and had a positive intravenous fluid balance > 4 L within 3 days. We assigned patients in a 1:1 ratio to the intervention protocol (intervention group) or usual care. The primary outcome was hospital-acquired HF. The secondary outcomes included in-hospital mortality, intensive critical unit (ICU) admission, mechanical ventilator usage, or prolonged hospital stay > 30 days.Results A total of 98 patients (mean age 66; 52% male) were enrolled (intervention group, 49; usual care group, 49). The incidence of hospital-acquired HF among all patients was 21%. Patients with hospital-acquired had higher rates of in-hospital mortality (48% vs.13%; p = 0.001), ICU admission (33% vs.10%; p = 0.010) and mechanical ventilator usage (62% vs. 35%; p = 0.027). Prolonged hospital stay > 30 days rates were similar in patients with and without hospital-acquired HF. Hospital-acquired HF was not found statistically different between groups (intervention group 18% vs. usual care group 25%; p = 0.460). Patients in the intervention group did have lower rates of subsequent ICU admission (8%vs.23%; p = 0.049) and hospitalizattion > 30 days. (8%vs.27%; p = 0.018) compared with the usual care group. In-hospital mortality and mechanical ventilator usage were not different between groups.Conclusions The incidence of hospital-acquired HF in patients with no preexisting cardiac dysfunction who had a positive cumulative fluid balance of > 4 L within 3 days was not uncommon, about one in five patients. Hospital-acquired HF can lead to increased in-hospital mortality and co-morbidities. Targeted fluid minimization and diuretics did show a reduced rate of ICU admission and prolonged hospitalization. However, no statistical difference in rates of hospital-acquired HF and in-hospital mortality compared to the control group were found.
Updated Distribution of Anopheline Mosquitoes (Diptera: Culicidae) in Hokkaido, Japan, and The First Evidence of Anopheles (Anopheles) Belenrae in Japan
Authors: Sawabe Kyoko , Imanishi-Kobayashi Nozomi , Maekawa Yoshihide , Higa Yukiko , Kim Kyeong Soon , Hoshino Keita , Tsuda Yoshio , Hayashi Toshihiko , Nihei Naoko , Takai Kenji , Kurihara Takeshi , Kobayashi Mutsuo 
Published: June 2021

Abstract
Background: After World War II in Hokkaido, northern island of Japan, at least seven cases of falciparum malaria were reported by 1951. A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very limited information on their current status and distribution in Japan. Therefore, this study is an update on the current status and distribution of anopheline mosquitoes in Hokkaido based on a 15-year entomological surveillance between 2001 and 2015. Methods: A survey of mosquitoes was conducted at 22 sites in Hokkaido, Japan, from 2001 to 2015. Adult mosquitoes were collected from cowsheds, lakesides, shrubs, and habitats ranging from open grassland to coniferous forest using a CDC miniature light trap enhanced with dry ice, aspirators, and sweeping nets. Larvae were collected from lakes, ponds, swamps, stagnant and flowing rivers, and paddy fields. All specimens were morphologically identified and subjected to PCR-based sequence analysis of the ITS2 region of rDNA. Phylogenetic trees were reconstructed using the neighbor-joining method. Results: A total of 46 anopheline specimens were used for the phylogenetic analysis. During the survey, a new member of the Anopheles hyrcanus group, An. belenrae Rueda (2005), was discovered in eastern Hokkaido in 2004. A nopheles belenrae has since then been consistently found and confirmed to inhabit only this area of Japan. Four members of the An. h yrcanus group, A n . belenrae , A n . engarensis , A n . lesteri , and An. sineroides , have been found in Hokkaido. The results also suggest that An. sinensis , formerly a dominant species throughout Japan, has become a rarely found species, at least currently in Hokkaido. Conclusion: The updated distribution of anopheline mosquitoes in Hokkaido, Japan, showed considerable differences from that observed in previous surveys conducted from 1969 to 1984. In particular, areas where An. sinensis was previously distributed may have been greatly reduced in Hokkaido. The phylogenetic analysis revealed a novel An. hyrcanus group member identified as An. belenrae , described in South Korea in 2005. It is interesting that An. belenrae was confirmed to inhabit only eastern Hokkaido, Japan.