bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of factors influencing tick bites and tick-borne infections: a longitudinal study
Authors: Jensen Bo Bødker , Bruun Mie Topholm , Jensen Per Moestrup , Pedersen Andreas Kristian , Fournier Pierre-Edouard , Skarphedinsson Sigurdur , Chen Ming 
Published: May 2021

Abstract
Background
Various tick-borne infections like borreliosis and rickettsiosis pose a health risk to humans in many parts of the world. We investigated seroprevalence of and seroconversion to Borrelia burgdorferi and Rickettsia spp. and relation to tick-bites, weather and clinical manifestations in Denmark.

Methods
Blood donors were enrolled at the Hospital of Southern Jutland in June–July with follow-up November–February of 2018 and 2019. Blood samples were collected, and a questionnaire regarding tick bites, potential exposures and symptoms was completed at each visit. Samples were tested for presence of IgM and IgG antibodies directed against B. burgdorferi and Rickettsia spp. using R. helvetica and R. felis as antigens. Data were examined for correlation between tick bites, serological results, potential exposures and symptoms.

Results
Two-hundred and fourteen (93 follow-ups) and 130 (38 follow-ups) blood donors were included in 2018 and 2019, respectively. The total borrelia seroconversion rate was 6.3% (CI 2.1–10.5), while the prevalence of IgM and IgG antibodies was 7.8% (CI 4.9–10.6) and 6.7% (CI 4–9.3), respectively. Seroconversion to Rickettsia spp. was detected in one participant. Tick bites and seroconversion were not significantly associated with the reported unspecific symptoms, but unspecific symptoms were common in the study population. There was no significant difference in number of tick bites or seroconversion/prevalence between seasons with highly alternating weather.

Conclusions
Results suggest that weather conditions in an individual year have a limited impact. Anti-Borrelia-antibodies do not seem to persist in serum for several years. Rickettsiosis is of limited concern in Denmark.

Graphical Abstract
IgG and IgA autoantibodies against L1 ORF1p expressed in granulocytes correlate with granulocyte consumption and disease activity in pediatric systemic lupus erythematosus
Authors: Ukadike Kennedy C. , Ni Kathryn , Wang Xiaoxing , Taylor Martin S. , LaCava John , Pachman Lauren M. , Eckert Mary , Stevens Anne , Lood Christian , Mustelin Tomas 
Published: May 2021

Abstract
Background
Most patients with systemic lupus erythematosus (SLE) have IgG autoantibodies against the RNA-binding p40 (ORF1p) protein encoded by the L1 retroelement. This study tested if these autoantibodies are also present in children with pediatric SLE (pSLE) and if the p40 protein itself could be detected in immune cells.

Methods
Autoantibodies in the plasma of pSLE patients (n = 30), healthy children (n = 37), and disease controls juvenile idiopathic arthritis (JIA) (n = 32) and juvenile dermatomyositis (JDM) (n = 60), were measured by ELISA. Expression of p40 in immune cells was assessed by flow cytometry. Markers of neutrophil activation and death were quantitated by ELISA.

Results
IgG and IgA autoantibodies reactive with p40 were detected in the pSLE patients, but were low in healthy controls and in JIA or JDM. pSLE patients with active disease (13 of them newly diagnosed) had higher titers than the same patients after effective therapy (p = 0.0003). IgG titers correlated with SLEDAI (r = 0.65, p = 0.0001), ESR (r = 0.43, p = 0.02), and anti-dsDNA antibodies (r = 0.49, p < 0.03), and inversely with complement C3 (r = -0.55, p = 0.002) and C4 (r = -0.51, p = 0.006). p40 protein was detected in a subpopulation of CD66b+ granulocytes in pSLE, as well as in adult SLE patients. Myeloperoxidase and neutrophil elastase complexed with DNA and the neutrophil-derived S100A8/A9 were elevated in plasma from pSLE patients with active disease and correlated with anti-p40 autoantibodies and disease activity.

Conclusions
Children with active SLE have elevated IgG and IgA autoantibodies against L1 p40, and this protein can be detected in circulating granulocytes in both pediatric and adult SLE patients. P40 expression and autoantibody levels correlate with disease activity. Markers of neutrophil activation and death also correlate with these autoantibodies and with disease activity, suggesting that neutrophils express L1 and are a source of p40.
Leptospirosis in febrile patients with suspected diagnosis of dengue fever
Authors: del Valle-Mendoza Juana , Palomares-Reyes Carlos , Carrillo-Ng Hugo , Tarazona-Castro Yordi , Kym Sungmin , Aguilar-Luis Miguel Angel , del Valle Luis J. , Aquino-Ortega Ronald , Martins-Luna Johanna , Peña-Tuesta Isaac , Verne Eduardo , Silva-Caso Wilmer 
Published: May 2021

Abstract
Objective
This study was carried out to determine the prevalence of leptospirosis among febrile patients with a suspicious clinical diagnosis of dengue fever in northern Peru.

Results
A total of 276 serum samples from patients with acute febrile illness (AFI) and suspected diagnosis for dengue virus (DENV) were analyzed. We identified an etiological agent in 121 (47.5%) patients, DENV was detected in 30.4% of the cases, leptospirosis in 11.2% and co-infection by both pathogens was observed in 5.9% of the patients. In this study the most common clinical symptoms reported by the patients were: headache 89.1%, myalgias 86.9% and arthralgias 82.9%. No differences in symptomatology was observed among the different study groups.
A dual cardiomyocyte reporter model derived from human pluripotent stem cells
Authors: Jiang Yuqian , Bao Xiaoping , Lian Xiaojun Lance 
Published: May 2021

AbstractCardiovascular diseases (CVD) remain the leading cause of death in the USA. Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. Combined with a fluorescent voltage indicator, ASAP2f, we achieved a dual reporter CM platform, which enables purification and characterization of CM subtypes and holds great potential for disease modeling and drug discovery of CVD.
Single-cell RNA sequencing of cultured human endometrial CD140b+CD146+ perivascular cells highlights the importance of in vivo microenvironment
Authors: Cao Dandan , Chan Rachel W. S. , Ng Ernest H. Y. , Gemzell-Danielsson Kristina , Yeung William S. B. 
Published: May 2021

Abstract
Background
Endometrial mesenchymal-like stromal/stem cells (eMSCs) have been proposed as adult stem cells contributing to endometrial regeneration. One set of perivascular markers (CD140b&CD146) has been widely used to enrich eMSCs. Although eMSCs are easily accessible for regenerative medicine and have long been studied, their cellular heterogeneity, relationship to primary counterpart, remains largely unclear.

Methods
In this study, we applied 10X genomics single-cell RNA sequencing (scRNA-seq) to cultured human CD140b+CD146+ endometrial perivascular cells (ePCs) from menstrual and secretory endometrium. We also analyzed publicly available scRNA-seq data of primary endometrium and performed transcriptome comparison between cultured ePCs and primary ePCs at single-cell level.

Results
Transcriptomic expression-based clustering revealed limited heterogeneity within cultured menstrual and secretory ePCs. A main subpopulation and a small stress-induced subpopulation were identified in secretory and menstrual ePCs. Cell identity analysis demonstrated the similar cellular composition in secretory and menstrual ePCs. Marker gene expression analysis showed that the main subpopulations identified from cultured secretory and menstrual ePCs simultaneously expressed genes marking mesenchymal stem cell (MSC), perivascular cell, smooth muscle cell, and stromal fibroblast. GO enrichment analysis revealed that genes upregulated in the main subpopulation enriched in actin filament organization, cellular division, etc., while genes upregulated in the small subpopulation enriched in extracellular matrix disassembly, stress response, etc. By comparing subpopulations of cultured ePCs to the publicly available primary endometrial cells, it was found that the main subpopulation identified from cultured ePCs was culture-unique which was unlike primary ePCs or primary endometrial stromal fibroblast cells.

Conclusion
In summary, these data for the first time provides a single-cell atlas of the cultured human CD140b+CD146+ ePCs. The identification of culture-unique relatively homogenous cell population of CD140b+CD146+ ePCs underscores the importance of in vivo microenvironment in maintaining cellular identity.
Senescence of donor cells impairs fat graft regeneration by suppressing adipogenesis and increasing expression of senescence-associated secretory phenotype factors
Authors: Chen Xihang , Feng Jingwei , Chang Qiang , Lu Feng , Yuan Yi 
Published: May 2021

Abstract
Background
Fat grafting has been regarded as a promising approach for regenerative therapy. Given the rapidly aging population, better understanding of the effect of age on fat graft outcomes and the underlying mechanisms is urgently needed.

Methods
C57/BL6 mice [old (O, 18–20-month-old) and young (Y, 4-month-old)] were randomized to four fat graft groups [old-to-old (O-O), young-to-young (Y-Y), old-to-young (O-Y), and young-to-old (Y-O)]. Detailed cellular events before and after grafting were investigated by histological staining, RNA sequencing, and real-time polymerase chain reaction. The adipogenic differentiation potential of adipose-derived mesenchymal stem cells (AD-MSCs) from old or young donors was investigated in vitro. Additionally, adipogenesis of AD-MSCs derived from old recipients was evaluated in the culture supernatant of old or young donor fat tissue.

Results
After 12 weeks, the volume of fat grafts did not significantly differ between the O-O and O-Y groups or between the Y-Y and Y-O groups, but was significantly smaller in the O-O group than in the Y-O group and in the O-Y group than in the Y-Y group. Compared with fat tissue from young mice, senescence-associated secretory phenotype (SASP) factors were upregulated in fat tissue from old mice. Compared with the Y-O group, adipogenesis markers were downregulated in the O-O group, while SASP factors including interleukin (IL)-6, tumor necrosis factor-α, and IL-1β were upregulated. In vitro, AD-MSCs from old donors showed impaired adipogenesis compared with AD-MSCs from young donors. Additionally, compared with the culture supernatant of young donor fat tissue, the culture supernatant of old donor fat tissue significantly decreased adipogenesis of AD-MSCs derived from old recipients, which might be attributable to increased levels of SASP factors.

Conclusions
Age has detrimental effects on fat graft outcomes by suppressing adipogenesis of AD-MSCs and upregulating expression of SASP factors, and fat graft outcomes are more dependent on donor age than on recipient age. Thus, rejuvenating fat grafts from old donors or banking younger adipose tissue for later use may be potential approaches to improve fat graft outcomes in older adults.
Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis
Authors: Kurosaka Mitsutoshi , Ogura Yuji , Sato Shuichi , Kohda Kazuhisa , Funabashi Toshiya 
Published: May 2021

AbstractBackgroundThe signal transducer and activator of transcription 6 (STAT6) transcription factor plays a vitally important role in immune cells, where it is activated mainly by interleukin-4 (IL-4). Because IL-4 is an essential cytokine for myotube formation, STAT6 might also be involved in myogenesis as part of IL-4 signaling. This study was conducted to elucidate the role of STAT6 in adult myogenesis in vitro and in vivo.MethodsMyoblasts were isolated from male mice and were differentiated on a culture dish to evaluate the change in STAT6 during myotube formation. Then, the effects of STAT6 overexpression and inhibition on proliferation, differentiation, and fusion in those cells were studied. Additionally, to elucidate the myogenic role of STAT6 in vivo, muscle regeneration after injury was evaluated in STAT6 knockout mice.ResultsIL-4 can increase STAT6 phosphorylation, but STAT6 phosphorylation decreased during myotube formation in culture. STAT6 overexpression decreased, but STAT6 knockdown increased the differentiation index and the fusion index. Results indicate that STAT6 inhibited myogenin protein expression. Results of in vivo experiments show that STAT6 knockout mice exhibited better regeneration than wild-type mice 5 days after cardiotoxin-induced injury. It is particularly interesting that results obtained using cells from STAT6 knockout mice suggest that this STAT6 inhibitory action for myogenesis was not mediated by IL-4 but might instead be associated with p38 mitogen-activated protein kinase phosphorylation. However, STAT6 was not involved in the proliferation of myogenic cells in vitro and in vivo.ConclusionResults suggest that STAT6 functions as an inhibitor of adult myogenesis. Moreover, results suggest that the IL-4-STAT6 signaling axis is unlikely to be responsible for myotube formation.
Anti-epileptic drug exposure during pregnancy and neonatal birth weight outcomes: protocol for a systematic review and meta-analysis
Authors: Lavu Alekhya , Vaccaro Christine , Shouman Walid , Severini Silvia Alessi , Eltonsy Sherif 
Published: May 2021

Abstract
Background
The prevalence of epilepsy in pregnant women is estimated at 0.3-1%. Anti-epileptic drug (AED) exposure in-utero has been associated with various adverse health outcomes in neonates, including adverse birth weight outcomes.

Objective
This review aims to summarize the published evidence on the association between AED exposure in pregnancy and adverse birth weight outcomes

Methods
Studies assessing AED exposure in pregnancy and neonatal birth weight outcomes, including small for gestational age (SGA), low birth weight (LBW), birth weight (BW), length, head circumference, and cephalization index will be identified in MEDLINE®, EMBASE, Cochrane Library, Scopus, Cumulative Index of Nursing and Allied Health Literature (CINAHL), International Pharmaceutical Abstracts (IPA), and Global Health. Open grey, Theses Canada, and ProQuest Dissertations will be used to locate gray literature. Eligible study designs will include both intervention and non-interventional studies. We will not impose any time limit in the review. We will use the Newcastle-Ottawa Scale to assess the methodological quality of observational studies and quasi-experimental studies included in the review. The risk of bias of experimental studies will be appraised using the Cochrane risk-of-bias tool for randomized trials (RoB 2). A meta-analysis will be conducted using a random-effects model.

Discussion
The results from this review could improve clinicians’ prescribing decisions by highlighting the safest AEDs for women who are pregnant or planning to conceive based on the evidence currently available.

Systematic review registration
PROSPERO CRD42020192713
On paranormed Ideal convergent sequence spaces defined by Jordan totient function
Authors: Khan Vakeel A. , Tuba Umme 
Published: May 2021

AbstractThe study of sequence spaces and summability theory has been an important aspect in defining new notions of convergence for the sequences that do not converge in the usual sense. Paving the way into the applications of law of large numbers and theory of functions, it has proved to be an essential tool. In this paper we generalise the classical Maddox sequence spaces $c_{0}(p)$

c
0

(
p
)
, $c(p)$
c
(
p
)
, $\ell (p)$
ℓ
(
p
)
and $\ell _{\infty }(p)$

ℓ
∞

(
p
)
and define new ideal paranormed sequence spaces $c^{I}_{0}(\Upsilon ^{r}, p)$

c
0
I

(

ϒ
r

,
p
)
, $c^{I}(\Upsilon ^{r}, p)$

c
I

(

ϒ
r

,
p
)
, $\ell ^{I}_{ \infty }(\Upsilon ^{r}, p)$

ℓ
∞
I

(

ϒ
r

,
p
)
and $\ell _{\infty }(\Upsilon ^{r}, p)$

ℓ
∞

(

ϒ
r

,
p
)
defined with the aid of Jordan’s totient function and a bounded sequence of positive real numbers. We develop isomorphism between certain maps and also find their α-, β- and γ-duals. We examine algebraic and topological properties of these corresponding spaces. Further we study some standard inclusion relations and prove the decomposition theorem.
Therapeutic effects of sesamolin on leukemia induced by WEHI-3B in model mice
Authors: Nagarajan Senthil , Lee Jae Kwon 
Published: May 2021

AbstractSesamolin is one of the lignans derived from sesame oil. It has demonstrated significant antioxidant, anti-aging, and anti-mutagenic properties. It also reportedly augments natural killer (NK) cell lysis activity. We previously reported that sesamolin also exerts anticancer effects in vitro and induces enhanced NK cell cytolytic activity against tumor cells. Herein, we aimed to determine the mechanism by which sesamolin prevents and retards tumorigenesis in BALB/c mouse models of leukemia induced by murine (BALB/c) myelomonocytic leukemia WEHI-3B cells. Banded neutrophils, myeloblasts, and monocytic leukemic cells were more abundant in the leukemia model than in normal mice. Sesamolin decreased the number of leukemic cells by almost 60% in the leukemia model mice in vivo; additionally, sesamolin and the positive control drug, vinblastine, similarly hindered neoplastic cell proliferation. Spleen samples were ~ 4.5-fold heavier in leukemic mice than those obtained from normal mice, whereas spleen samples obtained from leukemic mice treated with sesamolin had a similar weight to those of normal mice. Moreover, sesamolin induced a twofold increase in the cytotoxic activity of leukemic mouse NK cells against WEHI-3B cells. These results indicated that sesamolin exerts anti-leukemic effects in vivo.
Driving contradictions: behaviors and attitudes regarding handheld and hands-free cellphone use while driving among young drivers
Authors: Neuroth Lucas M. , Galos Dylan , Li Li , Zhao Songzhu , Zhu Motao 
Published: May 2021

Abstract
Background
Cellphone use while driving (CUWD) is a frequent source of distraction for young drivers. These distractions commonly lead to motor vehicle crashes and, in some cases, death. Crash risk differs depending on if the driver is engaging in handheld or hands-free CUWD. This pilot study sought to investigate the differences between handheld versus hands-free CUWD behaviors in younger drivers and the attitudes and social norms that inform them.

Methods
Young drivers (mean age: 19.6 years, standard deviation: 0.8 years) were recruited from a large Midwestern city in the United States as part of a pilot study. The 62 enrolled drivers (51 females, 43 non-Hispanic white) completed an online survey measuring behavioral frequencies, attitudes, and social norms regarding talking on the phone, sending messages, and reading messages. These cross-sectional data were then categorized and used for a descriptive analysis.

Results
The majority of young drivers reported participating in some form of handheld CUWD, with reading messages being the most popular (95%). Only 43% of participants used hands-free technology for sending messages and 30% for reading messages, while half reported not using the technology at all. Whereas handheld messaging behaviors were viewed negatively by the participants, they were unsure of the impact on their driving ability and the legality surrounding hands-free messaging behaviors.

Conclusions
Handheld CUWD behaviors were more popular among young drivers compared to hands-free CUWD. Further, even though young drivers understood handheld cellphone use while driving is unsafe, they engaged in it anyway. The findings of this pilot study highlight the importance of better educational initiatives and optimizing hands-free interventions for young driver use cases.
Various far-field hydrological responses during 2015 Gorkha earthquake at two distant wells
Authors: Huang Xudong , Zhang Yu 
Published: May 2021

AbstractAquifer hydraulic parameter can change during earthquakes. Continuous monitoring of the response of water level to seismic waves or solid Earth tides provides an opportunity to document how earthquakes influence hydrological properties. Here, we use data of two groundwater wells, Dian-22 (D22) and Lijiang (LJ) well, in southeast Tibet Plateau in response to the 2015 Mw 7.8 Gorkha earthquake to illustrate hydrological implications. The coherences of water level and seismic wave before and after the far-field earthquake show systematic variations, which may confirm the coseismic dynamic shaking influence at high frequencies (f > 8 cpd). The tidal response of water levels in these wells shows abrupt coseismic changes of both phase shift and amplitude ratio after the earthquake, which may be interpreted as an occurrence in the vertical permeability of a switched semiconfined aquifer in the D22 well, or an enhancement unconfined aquifer in the LJ well. Using the continuous short-term transmissivity monitoring, we show that the possible coseismic response for about 10 days and instant healing after 10 days to the causal earthquake impact. Thus, the dynamic shaking during the Gorkha earthquake may have caused the short-term aquifer responses by reopening of preexisting vertical fractures and later healing at epicentral distances about 1500 km.
Kusatsu-Shirane volcano eruption on January 23, 2018, observed using JMA operational weather radars
Authors: Sato Eiichi 
Published: May 2021

AbstractA phreatic eruption suddenly occurred at Motoshirane (Kusatsu-Shirane volcano, Japan) at 10:02 JST on January 23, 2018. A member of the Japan Self-Defense Force was killed by volcanic blocks during training in Motoshirane, and 11 people were injured by volcanic blocks or fragments of broken glass. According to a field survey, ash fall was confirmed in Minakami, about 40 km east-northeast from Motoshirane. Although the eruption was not captured by a distant camera, the eruption plume/cloud was captured by three of the Japan Meteorological Agency’s operational weather radars. These radars observed the echo propagated to the northeast in the lower troposphere, and to the east in the middle troposphere. This is generally consistent with the observed ash fall distribution. Using the modified probabilistic estimation method, the maximum plume height was estimated to be about 5580 ± 506 m (1σ) above sea level. Estimates of the erupted mass based on the range of plume heights from radar observations and the duration of volcanic tremor during the eruption (about 8 min) do not match that obtained from a field survey (3.0–5.0 × 107 kg). This discrepancy confirms that estimates of erupted mass based on plume heights must account for eruption style parametrically, which can only be constrained by case studies of varied eruption styles.