bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Activated Factor VII in Aortic Surgery for Patients With Coagulation Disorders and Hypothermic Circulatory Arrest: a Single-center Retrospective Study
Authors: Ise Hayato , Ushioda Ryohei , Kanda Hirotsugu , Kimura Fumiaki , Saijo Yasuaki , Akhyari Payam , Lichtenberg Artur , Kamiya Hiroyuki 
Published: October 2021

Abstract
Background: We aimed to identify the risk factors of uncontrollable bleeding and investigate the safety of recombinant activated factor VII (rFVIIa) in aortic surgery under hypothermic circulatory arrest (ASHCA).Methods: The present single-center retrospective study compared the baseline characteristics of 144 patients who underwent ASHCA at our institute the total cohort. Of the 144 patients, 42 received rFVIIa (group rFVIIa), while the remaining 102 patients did not (group non-rFVIIa). Perioperative bleeding and transfusions, postoperative 30-day mortality, and adverse events (AEs) were analyzed in 29 propensity score-matched pairs.Results: Before surgery, the rFVIIa group demonstrated a greater number of shocks (p=0.019), higher JapanSCOREx II mortality rate (p=0.033), low platelet count (p=0.015), fibrinogen (p<0.001), prolonged activated partial thromboplastin time (p=0.005), prothrombin time international normalized ratio (p=0.006), and longer aortic cross cramp (ACC) time (p=0.049). Postoperative bleeding, transfusion, 30-day mortality, and AEs were comparable between the groups.Conclusions: Preoperative shock, higher JapanSCORE II mortality rates, low platelet and fibrinogen levels, prolonged aPTT and PT-INR, and longer ACC time might be risk factors for excessive bleeding and indicate the need for rFVIIa treatment. The present study suggests that rFVIIa can be safely used to address uncontrollable bleeding in ASHCA without inducing an increase in 30-day mortality and AEs.
Yeasts Associated With The Worker Caste of The Leaf-Cutting Ant Atta Cephalotes Under Experimental Conditions In Colombia
Authors: Giraldo Carolina , Chaves-López Clemencia , Tofalo Rosanna , Angrisani Roberto , Rodrigues Andre , Montoya-Lerma James 
Published: October 2021

Abstract
Yeasts isolated from the worker caste of the Colombian leaf-cutting ant, Atta cephalotes (Hymenoptera: Myrmicinae) were cultured and identified by molecular methods. Abundant, persistent and omnipresent species were classified as “prevalent”. Experimental data were compared with information gathered from published reports on the yeast species composition in other leaf-cutting ant species. Diversity analysis was conducted using diversity values (q0, q1, and q2) to compare richness and abundance of yeasts present in different leaf-cutting ant species Clustering analysis was carried out to assess similarity of yeast community according to ant species. The yeast species composition was highly variable among the ant species. Atta laevigata and A. capiguara showed the highest degree of similarity and differed from the group composed by A. cephalotes, A. sexdens, A. sexdens rubropilosa, and A. texana. The isolation of dominant yeasts in different ant castes within the different compartments of a colony strongly suggests that the identified microorganisms are not transient but are native to the soil surrounding ant colonies and the substrates used by the ants to grow their fungal cultivars. It is apparent that the ant-fungus mutualism does not operate in an environment devoid of other microbes, but rather that the association must be seen within the context of a background of other microorganisms, particularly the dominant yeasts.
P4HA1 Acts as a Potential Target in Renal Cell Carcinoma and Pan-cancer
Authors: Li Yang , Ge Yu-zheng , Qian Yi-guan , Chen Ke , Zhao Feng , Qin Zhiqiang , Zhou Liuhua , Xu Luwei , Xu Zheng , Dou Quanliang , Jia Ruipeng 
Published: October 2021

Abstract
Background: Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) plays a vital role in providing the major part of the catalytic site of the active enzyme. Emerging evidence has revealed that P4HA1 participates in the initiation and development of several malignant tumors, nevertheless, the pan-analysis of P4HA1 remains unspecified. Methods: In this study, we carried out an in-depth analysis of the expression patterns and prognostic value of P4HA1 across the datasets of The Cancer Genome Atlas (TCGA ) and Kaplan-Meier Plotter. The genomic and epigenetic alterations, correlation with immune infiltration and DNA methylation of P4HA1 in different cancers were also analyzed across multiple databases. Moreover, we further explored the biological function and mechanism of P4HA1 in renal cell carcinoma (RCC). Results: The results characterized the different expression profile and prognostic values of P4HA1 in pan-cancer. Remarkably, P4HA1 expression was increased in kidney renal clear cell carcinoma (KIRC) and positively correlated with the OS and DFS of patients with kidney renal papillary cell carcinoma (KIRP). In addition, a positive correlation between P4HA1 expression and immune cell infiltration in KIRC was observed. We also identified a strong correlation between P4HA1 expression and immune checkpoint gene expression, microsatellite instability and tumor mutation burden in kidney chromophobe (KICH). Finally, the results of in vitro experiments verified that overexpression of P4HA1 promoted the proliferation, migration, invasion and the epithelial–mesenchymal transition (EMT) of RCC cells. Conclusion: Overall, our study suggested that P4HA1 might play a significant role in tumorigenesis and have the potential to be the target for the prognosis and therapy for several malignant tumors, especially for RCC.
A Death-Related Gene Signature for Prognosis with Osteosarcoma
Authors: Xie Bin , Tan Shiyong , Li Chao , Liang Junyang 
Published: October 2021

Abstract
Purpose: Osteosarcoma is one of the most prevalent malignancies, and despite significant advances in its treatment, patient prognosis remains poor and survival rates are low. It is undoubtedly important to explore the possible reasons for the low survival rates of patients and to reveal the differences.Methods and Results: We obtained RNA-Seq (HT seq) and clinical characteristics of osteosarcoma patients from the TCGA database and divided them into survival group and death group. We defined the differentially expressed genes (DEGs) between the two groups as death-related genes (DRGs) and used them to construct a prognostic signature for overall survival of patients with osteosarcoma. The results of the validation demonstrated satisfactory accuracy and predictive prognostic value of the model. In addition, we performed a series of bioinformatic analyses that identified two key genes and the regulatory networks they constituted that may play a role in the progression of osteosarcoma.Conclusion: Our DRGs signature represents a novel and clinically useful prognostic biomarker for patients with osteosarcoma, helping to aid clinical decision-making.
Effects of an Early Mass-flowering Crop on Wild Bee Communities and Traits in Power Line Corridors Vary With Floral Resources and Landscape Context
Authors: Du Clos Brianne , Drummond Francis A. , Loftin Cynthia S. 
Published: October 2021

Abstract
ContextPower line corridors have been repeatedly assessed as habitat for wild bees; however, few studies have examined them as bee habitat relative to nearby crop fields and surrounding landscape context.ObjectivesWe surveyed bee communities in power line corridors near (within 150-300 m) to and isolated (>1 km) from lowbush blueberry fields in both a simple and a complex landscape context. Additionally, we examined the influences of floral resources and bee life-history traits. MethodsWe surveyed wild bees and floral resources in power line corridors in Maine, USA, during 2013-2015. We calculated landscape composition surrounding sites at four spatial scales and gathered bee trait information from the literature. We assessed differences in bee communities across growing regions and site types with generalized linear models.ResultsWe collected many of Maine’s wild bee species and observed a rare plant-pollinator relationship within power line corridors. We found greater bee abundance and species richness throughout the more complex Midcoast landscape, while our expected enhancement from mass-flowering lowbush blueberry fields was evident only with bee species richness in the simpler Downeast landscape. Landscape composition and floral diversity varied between the two growing regions, though landscape composition had more influence over bee communities than floral diversity. Solitary species and ground-nesting species were more sensitive to landscape context than social or cavity-nesting species. ConclusionsPower line corridors provide crucial refugia for crop pollinating wild bees in resource-poor landscapes. Further exploration of population dynamics and pollination efficiency may clarify this relationship.
Fabrication Mechanism of Asymmetric Block Copolymer Membrane: Combination of Self-Assembly and Phase Separation Process
Authors: Hamta Afshin , Ashtiani Farzin Zokaee , Karimi Mohammad , Moayedfard Sareh 
Published: October 2021

Abstract
In this paper, the concept of functional mechanism of copolymer membrane formation is explained and analyzed from theoretical and experimental point of view. To understand the phase inversion process and control the final membrane morphology, styrene-acrylonitrile copolymer (SAN) membrane morphology through the self-assembly phenomena is investigated. Since the analysis of the membrane morphology requires the study of both thermodynamic and kinetic parameters, the effect of different membrane formation conditions is investigated experimentally; In order to perceive the formation mechanism of the extraordinary structure membrane, a thermodynamic hypothesis is also developed based on the hydrophilic coil migration to the membrane surface. This hypothesis is analyzed according to Hansen Solubility Parameters and proved using EDX, SAXS and contact angle analysis of SAN25. Moreover, the SAN30 membrane is fabricated under different operating conditions to evaluate the possibility of morphological prediction based on the developed hypothesis.
Implementing Food Bank and Health Care Partnerships: A Pilot Study of Perspectives From Charitable Food Systems in Texas
Authors: Poulos Natalie , Nehme Eileen , O'Neil Molly , Mandell Dorothy 
Published: October 2021

Abstract
Background

Partnerships between charitable food systems and healthcare systems have been forming across the country to support individuals and families experiencing food insecurity, yet little research has focused on these partnerships, particularly from a food bank perspective. The objective of this exploratory pilot study was to identify implementation challenges and facilitators of charitable food system and healthcare partnerships from the food bank perspective.
Method

Texas food banks with existing food bank/healthcare partnerships were identify through website review and support from Feeding Texas. Interview questions were tailored to each interview, but all focused on identify program components of the food bank/healthcare partnership and implementation barriers/facilitators of the partnership. In total, six interviews were conducted with food bank/healthcare partnership leaders (n=4) and charitable food system experts (n=2) about their experiences of working with food bank/healthcare partnerships. All interviews were completed via Zoom and took between 30-60 minutes to completed. Detailed notes were taking during each interview, and immediately discussed with the complete research time to formulate broad implementation themes.
Results

Interviews suggest unique implementation challenges exist at all levels of food bank/healthcare partnerships including the partnership, program, and system levels. Partnership-level implementation challenges focused on issues of partnership scale and data collection, sharing, and analysis. Program-level implementation challenges focused on food and produce expectations. Structural-level implementation challenges included issues of food safety, subsidized food regulations, and patient privacy. Implementation facilitators included leadership support, mission compatibility/organizational readiness, food insecurity training, and identify of partnership champions.
Conclusions

This study adds to the growing interest in food bank/healthcare partnership as it highlights unique implementation challenges and facilitators for cross-sector partnerships between healthcare systems and community-based charitable food systems. Ultimately, we believe that collaborative discussion among leaders of charitable food systems and healthcare systems is needed to overcome outlined implementation challenges to better facilitate sustainable, equitable implementation of food bank/healthcare partnerships.
Imaging the Alternatively Spliced D Domain of Tenascin C in Preclinical Models of Inflammatory Bowel Disease
Authors: Zhang Liang , Pohl Calvin S. , Homan Kristoff T. , Sun Victor Z. , Gaudette Stephanie M. , Nelson Christine M. , Erickson Jamie E. , Knight Heather L. , Bose Sahana , Lynch Grace R. , McRae Bradford L. , Sterman Annette J. Schwartz , Mitra Soumya 
Published: October 2021

Abstract
Purpose To image colon-expressed alternatively spliced D domain of tenascin C in preclinical colitis models using near infrared (NIR) labeled targeted molecular imaging agents.Methods Human IgG and scFv fusion proteins specific to the alternatively spliced D domain of tenascin C were generated. Immunohistochemistry identified disease-specific expression of this extracellular matrix in mouse colitis models. Proteins were labeled with the NIR fluorophore IRDye 800CW via amine chemistry and intravenously dosed to evaluate targeting specificity in preclinical rodent and primate colitis models.Results The NIR labeled proteins successfully targeted colonic lesions in a murine model of colitis and appeared as distinct punctate spots. Co-administration of a blocking dose reduced the whole colon standardized uptake of the fluorescent dose >7-fold in mouse models. Estimates suggest local expression at >100 nM in diseased mouse colon. Macroscopic targeting specificity was not observed in diseased primate colon. Cellular level specificity was assessed via microscopy and immunohistochemistry.Conclusion Our imaging data suggest the alternatively spliced D domain of tenascin C is a promising target for delivery-based applications in inflammatory bowel diseases.
Association Studies of Different Genetic Variants of αS2-casein Gene (CSN1S2) With Milk Production Traits (Lactose, SNF&amp;Density) in Malvi, Nimari, Sahiwal &amp; HF Crossbred Cattle
Authors: Pandey Akhilesh , Khan Zeeshan 
Published: October 2021

Abstract
αs2-casein is a primary milk protein. However, there has been little research focused on the effects of αs2-casein variants on milk. The αs2-casein (CSN1S2) play a major protein found in ruminant’s milk, which is encoded by a highly polymorphic CSN1S2 gene present on chromosome 6. Research work was carried out on 50 animals each of Malvi, Nimari, Sahiwal and HF Crossbred cattle at Jabalpur, Madhya Pradesh, India. The αS2 casein gene (1267 bp) was digested by EcoRV restriction enzyme yielding two genotypes viz., AA (1267 bp) and BB (1267/1150/117 bp) in Malvi, Nimari and HF crossbred cattle, while only one genotype (AA) in Sahiwal. The restriction site was absent in Sahiwal resulting in only single compact band of 1267 bp. The tested animals of Sahiwal were found monomorphic at this locus. Association study of different genotypes with milk composition traits revealed that the mean lactose % was significantly higher in Malvi compared to others for AB genotype animals as compared to AA genotype. The mean SNF % between AA and AB was found non-significant in Malvi, Nimari and HF crossbred. The mean milk density (kg/L) was significantly higher for AB genotype than AA genotype of Nimari and HF crossbred. The mean milk density (kg/L) was observed maximum in Nimari for AB genotype and minimum in Malvi and Sahiwal for AA genotype. The genotypic and gene frequencies of αS2-casein gene (CSN1S2)/EcoRV locus pattern showed that the frequencies of AA, AB and BB genotypes were found to be 0.44, 0.56 and 0.00 in Malvi; 0.68, 0.32 and 0.00 in Nimari; 1.00, 0.00, and 0.00 in Sahiwal and 0.58, 0.42 and 0.00 in HF crossbred, respectively. The frequency of A allele was found to be highest as compared to B allele in all the four breeds of cattle under the study.
Assessment of Biosafety and Implantation Feasibility of Novel Phakic Refractive Lens
Authors: Zhang Shaohua , Huang Chang , Miao Huamao , Wu Junyao , Xing Chao , Dai Zhaoxing , Sun Jianguo 
Published: October 2021

Abstract
Purpose: To investigate the biosafety and implantation feasibility of newly developed phakic refractive lens (PRL) in rabbit eyes. Methods: The PRLs, including short PRL (S-PRL), large PRL (L-PRL), and large grooved PRL (LG-PRL), were prepared by molding liquid medical silicon. The in vitro cytotoxicity of the above PRLs was evaluated by incubating them with human lens epithelial cells (HLECs) and then measuring cell viability by CCK-8 assay. In vitro cell adhesion of PRLs was assessed by culturing HLECs on PRL film surface and calculating the cell number and average cell area after stained with Calcein-AM and fluorescent. The implantation feasibility was appraised by observing the relative positions of S-PRL, L-PRL or LG-PRL implanted in the posterior chamber of rabbit eyes by optical coherence tomography, and calculating their retention ratio postoperatively. The intraocular pressure (IOP) of S-PRL, L-PRL, LG-PRL and control groups of rabbit eyes was compared to evaluate the biosafety of implantation.Results: The results of in vitro cytotoxicity showed no significant difference of cell viability was observed in the S-PRL, L-PRL or LG-PRL groups compared to the control group throughout the whole experiment. The HLECs cultured on the PRL film surface presented similar cell number, but smaller average cell area (53.8% vs 100%) when compared to the control group, which implied obvious adhesion inhibition on HLECs caused by PRL film. After implantation of S-PRL, L-PRL or LG-PRL into the posterior chamber of rabbit eyes, no obvious inflammation and IOP elevation were observed at each time point in all sample groups compared to the control group, which indicated that PRL samples had good implantation biosafety. Most of the implanted L-PRL and LG-PRL kept in the correct location, while only less of the S-PRL was at the right site. That was, L-PRL and LG-PRL had proper relative position and high retention ratio in the posterior chamber of rabbit eyes. L-PRL and S-PRL samples tended to attach to iris surface, while LG-PRL sample constructed enough space on the iris surface by its grooves surrounding the central optical zone, which was conducive to circulation of aqueous humor.Conclusions: The newly developed LG-PRL sample presented good biosafety in terms of the negligible in vitro cytotoxicity, ocular inflammation and IOP fluctuations. The LG-PRL provided the best implantation feasibility due to the more proper relative position, available space for aqueous humor circulation, and high retention ratio in the posterior chamber of rabbit eyes among the three kinds of PRL samples. Thus, LG-PRL is a promising alternative with appropriate size and surface structure to more effectively correct refractive errors.
Investigation of Patient Care Management in Fangcang Shelter Hospital under COVID-19: a Simple Electric Management Mode
Authors: Li Xueyuan , Chong Wei 
Published: October 2021

Abstract
The COVID-19 pandemic spreads all over the world for its high infectivity. This paper presents the practice in patient care management in a Fangcang shelter hospital(FSH) in Wuhan. Large numbers of mild patients were admitted to fangcang shelter hospitals at the beginning of 2020. The Liaoning National Medical Rescue Team (LNMRT) applied simple electronic management (SEM) mode in patient care management when working in the Wuchang Fangchang Shelter Hospital (WFSH). The medical efficiency was assessed by comparing the median hospitalization time between the patients receiving normal treatment and care (group A) and those receiving the SEM(group B). The median hospitalization time was 14 days for group A and 12 days for group B (p=0.001). As a result, It was more effective to apply SEM in fangcang shelter hospitals.
Mechanical Reliability and Modeling of Hydroxyapatite Scaffold
Authors: Abifarin Johnson Kehinde 
Published: October 2021

Abstract
Significant contributions on the improvement of the mechanical properties of hydroxyapatite (HAp) have been widely reported. However, failure analysis (mechanical reliability) and modeling are missing. This article filled the gap by conducting Two-parameter Weibull distribution assisted by modeling to investigate the mechanical reliability of HAp. The employed HAp was characterized under SEM/EDS analysis. The results revealed the characteristics of HAp and also the nature of the synthesis route employed through its irregular morphology. The Two-parameter Weibull distribution analysis was conducted on the hardness and compressive strength of HAp scaffold. The characteristic hardness and compressive strength, coupled with their corresponding bounds, failure rates, and correlation coefficients were been presented. The Weibull analysis with the assistance of modeling revealed HAp fabricated under 10 KN compaction load and sintered at 1100 oC as the most reliable sample under hardness condition, while HAp fabricated under 15 KN compaction load and sintered at 1000 oC gave the most reliable characteristic under compression. However, 15 KN compaction load and 1100 oC sintering temperature showed the best reliability on the overall mechanical (hardness and compressive strength) reliability. Future study is recommended on the reliability of HAp scaffolds considering other mechanical properties that are essential for biomedical application.
Changes in Left Ventricular Strain Parameters After Empagliflozin Treatment in Patients with Diabetes Mellitus and Normal Ejection Fraction
Authors: Işık Ferhat , Aslan Burhan , Bilge Önder , İnci Ümit , Taştan Ercan , Akçay Medet , Yıldız Halil , Demir Muhammed , Özbek Mehmet , Akın Halil , Araç Eşref 
Published: October 2021

Abstract
Objective: In recent years, sodium-glucose cotransporter-2 (SGLT-2) inhibitors are known to improve symptoms and reduce mortality in patients with heart failure (HF). Empagliflozin is an antidiabetic drug that SGLT-2 inhibitor. Empagliflozin has beneficial cardiac effects like other SGLT-2 inhibitors. However, data on how empagliflozin treatment affects echocardiographic parameters are limited. We aim to evaluate the changes in left ventricular myocardial strain parameters with 2-dimensional speckle tracking echocardiography (2D-STE) in patients with diabetes mellitus and normal ejection fraction after empagliflozin treatment.Material and Method: A total of 48 patients were included in our study. The left ventricular ejection fraction (LVEF) of the patients were normal and there were no HF symptoms and findings. Demographic, clinical, and echocardiographic parameters of study population were evaluated. Empagliflozin 10 mg once daily was given to all patients. Initial and at the end of 3rd month, the 2D-STE parameters of all patients were examined.Results: The median age of the study population was 55.0 (45.0 – 62.5, IQR) years and 27 (56.3 %) of them were female. There was a significant increase in the left ventricle global longutidunal strain (LV-GLS) and left ventricle global circumferential strain (LV-GCS) compared to baseline [respectively; -19.7(-17.5;-20.9) vs -19.2(-17.6;-20.2), p= 0.016 and -18.9(-16.0;-20.8) vs -17.1(-15.8;-18.7), p= 0.003]. Although left ventricular global radial strain (LV-GRS) was increased compared to baseline, it was not significant [37.0(31.0-41.6) vs 36.3(32.4-40.3), p= 0.776].Conclusion: In our study, left ventricular myocardial strain parameters such as LV-GLS and LV-GCS were improved in diabetic patients with normal ejection fraction after empagliflozin treatment.