bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contents of lobetyolin, syringin, and atractylolide III in Codonopsis pilosula are related to dynamic changes of endophytes under drought stress
Authors: Liang Yichuan , Wei Guangfei , Ning Kang , Zhang Guozhuang , Liu Youping , Dong Linlin , Chen Shilin 
Published: November 2021

Abstract
Background
Codonopsis pilosula, an important medicinal plant, can accumulate certain metabolites under moderate drought stress. Endophytes are involved in the metabolite accumulations within medicinal plants. It is still unknown that the endophytes of C. pilosula are associated with the accumulations of metabolites. This study aims to investigate the promoting effect of endophytes on the accumulations of active substances in C. pilosula under drought stress.

Methods
High–performance liquid chromatography and high–throughput sequencing technology were performed to investigate changes in the contents of secondary metabolite and endophyte abundances of C. pilosula under drought stress, respectively. Spearman’s correlation analysis was further conducted to identify the endophytic biomarkers related to accumulations of pharmacodynamic compounds. Culture-dependent experiments were performed to confirm the functions of endophytes in metabolite accumulations.

Results
The distribution of pharmacological components and diversity and composition of endophytes showed tissue specificity within C. pilosula. The contents of lobetyolin, syringin, and atractylolide III in C. pilosula under drought stress were increased by 8.47%‒86.47%, 28.78%‒230.98%, and 32.17%‒177.86%, respectively, in comparison with those in untreated groups. The Chao 1 and Shannon indices in different parts of drought–stressed C. pilosula increased compared with those in untreated parts. The composition of endophytic communities in drought treatment parts of C. pilosula was different from that in control parts. A total of 226 microbial taxa were identified as potential biomarkers, of which the abundances of 42 taxa were significantly and positively correlated to the pharmacodynamic contents. Culture-dependent experiments confirmed that the contents of lobetyolin and atractylolide III were increased by the application of Epicoccum thailandicum, Filobasidium magnum, and Paraphoma rhaphiolepidis at the rates of 11.12%‒46.02%, and that the content of syringin was increased by Pseudomonas nitroreducens at the rates of 118.61%‒119.36%.

Conclusions
Certain endophytes participated in the accumulations of bioactive metabolites, which provided a scientific evidence for the development and application of microorganisms to improve the quality of traditional Chinese medicine.
Assessment of catatonia and inter-rater reliability of three instruments: a descriptive study
Authors: Zingela Zukiswa , Stroud Louise , Cronje Johan , Fink Max , van Wyk Stephan 
Published: November 2021

Abstract
Background
Clinical assessment of catatonia includes the use of diagnostic systems, such as the Diagnostic and Statistical Manual, Fifth Edition (DSM-5) and the International Classification of Disease, Tenth Revision (ICD-10), or screening tools such as the Bush Francis Catatonia Screening Instrument (BFCSI)/Bush Francis Catatonia Rating Scale (BFCRS) and the Braunig Catatonia Rating Scale. In this study, we describe the inter-rater reliability (IRR), utilizing the BFCSI, BFCRS, and DSM-5 to screen for catatonia.

Methods
Data from 10 participants recruited as part of a larger prevalence study (of 135 participants) were used to determine the IRR by five assessors after they were trained in the application of the 14-item BFCSI, 23-item BFCRS, and DSM-5 to assess catatonia in new admissions. Krippendorff’s α was used to compute the IRR, and Spearman’s correlation was used to determine the concordance between screening tools. The study site was a 35-bed acute mental health unit in Dora Nginza Hospital, Nelson Mandela Bay Metro. Participants were mostly involuntary admissions under the Mental Health Care Act of 2002 and between the ages of 13 and 65 years.

Results
Of the 135 participants, 16 (11.9%) had catatonia. The majority (92 [68.1%]) were between 16 and 35 years old, with 126 (93.3%) of them being Black and 89 (66.4%) being male. The BFCRS (complete 23-item scale) had the greatest level of inter-rater agreement with α = 0.798, while the DSM-5 had the lowest level of inter-rater agreement with α = 0.565. The highest correlation coefficients were observed between the BFCRS and the BFCSI.

Conclusion
The prevalence rate of catatonia was 11.9%, with the BFCSI and BFCRS showing the highest pick-up rate and a high IRR with high correlation coefficients, while the DSM-5 had deficiencies in screening for catatonia with low IRR and the lowest correlation with the other two tools.
Clinical practice during the COVID-19 pandemic: a qualitative study among child and adolescent psychiatrists across the world
Authors: Sibeoni Jordan , Manolios Emilie , Costa-Drolon Emmanuel , Meunier Jean-Pierre , Verneuil Laurence , Revah-Levy Anne 
Published: November 2021

AbstractBackgroundThe COVID-19 pandemic has directly impacted the field of child and adolescent psychiatry, affecting all aspects of the lives of children and their families and increasing their risk of distress and mental health issues, especially among children with preexisting psychiatric disorders. Child and adolescent psychiatrists (CAPs) across the world have had to adapt their practice, due to lockdown and social distancing measures. This study aimed to explore how CAPs experienced their clinical practice in these singular conditions.MethodsThis exploratory international qualitative study used the Inductive Process to analyse the Structure of lived Experience (IPSE) approach, which is a five-stage inductive process used to explore the lived experience of participants in depth and to analyze their structure of lived experience. This study took place from March through July 2020 through individual in-depth video interviews. The sample size was determined according to the principles oftheoretical sufficiency.Results39 CAPs from 26 countries participated (age range 32–70 years; 23 women). Data analysis produced a structure of lived experience comprising three central axes of experience: (1) lost in space, lost in time, describing CAPs’ experience of disorganization of their clinical practice in the dimensions of lived time and lived space, (2) the body—of CAPs and patients—underlining their disconcerting experience of both sensory aspects and the non-embodied encounter during clinical practice, and (3) unpleasant emotions, with angst and loneliness the two main feelings coloring their clinical practice experience.ConclusionsThis analysis of the structure of lived experience of CAPs went beyond the sole context of the pandemic and revealed key aspects of what usually organizes CAP clinical practice. It identified two blind spots or conceptual voids within the child and adolescent psychiatry field: first, the intrinsic therapeutic function of a CAP clinical practice and, second, the important diagnostic and therapeutic function of the embodied encounter during CAP consultations. Beyond the context of COVID-19, further research should investigate these aspects to better define what a CAP does in practice and to increase both attractiveness and recruitment in this specialty.
Undescended ovary without abnormal development of uterus and urinary system: a report of four cases
Authors: Wei Weixia , Luo Wenji , Hu Qicai , Zeng Liping , Wu Ruifang 
Published: November 2021

Abstract
Background
Congenital anatomic abnormalities of fallopian tubes and ovaries are rarely reported. Herein, we describe four cases of undescended ovary during laparoscopic surgery with abnormal anatomy of fallopian tube, yet without abnormal uterine development and urinary system abnormalities, which are analyzed by their clinical features and effects on reproductive function.

Case presentation
For the patients with undescended ovary, the location of unilateral or bilateral upper poles of the ovaries were usually much higher than that of the bifurcation of the common iliac vessel, and the fallopian tubes at the same side opened in the para-colonic sulcus. Among these four patients, two patients had primary infertility, one patient had tubal pregnancy rupture and bleeding, and one patient had uterine leiomyoma. The development of uterus was normal in all cases, and there was no abnormal development of urinary system. During the infertility examination, the fact that fallopian tubes lifted up in hysterosalpingography (HSG) might be regarded as an indicator of possible undescended ovary. The pelvic ultrasonography examination was of limited use in diagnosing undescended ovary.

Conclusion
Laparoscopy is the gold standard for the diagnosis of undescended ovary. When there is periodic post-sacral spinal pain, MRI or HSG can be used for diagnosis of undescended ovary.
Impacts of different intensities of exercise on inflammation and hypoxia markers in low altitude
Authors: Baygutalp Fatih , Buzdağlı Yusuf , Ozan Murat , Koz Mitat , Kılıç Baygutalp Nurcan , Atasever Gökhan 
Published: November 2021

AbstractBackgroundThis study aims to determine and compare the effects of exercise modalities with different intensities on the secretion of key inflammation and hypoxia markers in amateur athletes.MethodsTwenty-three athletes with a mean age of 20.1 years, living at low altitude (1850 m) participated in this study. The participants' maximal oxygen consumption values (VO2max) were determined with an incremental cycle exercise test as 54.15 ± 6.14 mL kg min−1. Athletes performed four protocols: at rest, 50% VO2max, 75% VO2max and 100% VO2max (until exhaustion) with one-week intervals. 50% VO2max, 75% VO2max sessions were performed continuously for 30 min on a bicycle ergometer and 100% VO2max session was performed by cycling until exhaustion. Blood samples were obtained at rest and immediately after each exercise session. Serum tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP), interleukin-10 (IL-10), and hypoxia inducible factor-1 alpha (HIF-1α) levels were measured.ResultsThere were significant differences in serum TNF-α levels in 75% VO2max and 100% VO2max sessions (489.03 ± 368.37 and 472.70 ± 365.21 ng/L, respectively) compared to rest conditions (331.65 ± 293.52 ng/L). Serum CRP levels of 50% VO2max and 75% VO2max sessions (1.19 ± 0.50; 1.07 ± 0.52 mg/L) were significantly higher than the rest condition (0.74 ± 0.35 mg/L). There were significant differences in serum IL-10 levels of rest condition and 50% VO2max; 50% VO2max, and 100% VO2max sessions (328.09 ± 128.87; 446.36 ± 142.84; 347.44 ± 135.69; 324.88 ± 168.06 pg/mL). Serum HIF-1α levels were significantly higher in 75% VO2 max session compared to rest (1.26 ± 0.16; 1.08 ± 0.19 ng/mL) (P < 0.05 for all comparisons).ConclusionsBoth inflammatory and anti-inflammatory pathway is induced on different exercise intensities. Exercise protocols performed until exhaustion may lead to activation of inflammatory pathways and hypoxia-induced damage.
Afatinib for the treatment of advanced non-small-cell lung cancer harboring an epidermal growth factor receptor exon 18 E709_T710delinsD mutation: a case report
Authors: Van Acker Lander , Stevens Dieter , Vermaelen Karim , Surmont Veerle 
Published: November 2021

Abstract
Background
The discovery of epidermal growth factor receptor oncogenic driver mutations has changed the therapeutic landscape of advanced non-small cell lung cancer in the past decade. Since the introduction of next-generation sequencing, uncommon epidermal growth factor receptor mutations are more frequently discovered. Because seldom evaluated in clinical trials, their clinical significance and response on tyrosine kinase inhibitors are less well known.

Case presentation
A 58-year-old Caucasian woman with no smoking history presented with advanced non-small cell lung cancer. Liver biopsy revealed an adenocarcinoma with a programmed death ligand-1 tumor proportion score of 30% and no common oncogenic driver mutations. A combination of chemotherapy and immunotherapy was started as first-line treatment. However, treatment was ceased after 18 weeks because of immune-related renal failure and disease progression. In the meantime, the next-generation sequencing results of the liver biopsy had revealed an exon 18 E709_T710delinsD mutation. Therefore, afatinib was administered, which was moderately tolerated with grade 2 paronychia and acneiform skin eruption. After 6 months, a partial response with ongoing decrease of the liver metastasis was retained.

Conclusion
Because of the lack of clinical trials, tumor heterogeneity, and a tyrosine kinase inhibitor affinity related to the different mutation types, it is difficult to predict the clinical outcome of tyrosine kinase inhibitor in uncommon mutations. Therefore, a therapeutic trial with tyrosine kinase inhibitor has to be considered, but the expected clinical response is lower than for common mutations.
Six1 promotes skeletal muscle thyroid hormone response through regulation of the MCT10 transporter
Authors: Girgis John , Yang Dabo , Chakroun Imane , Liu Yubing , Blais Alexandre 
Published: November 2021

Abstract
Background
The Six1 transcription factor is implicated in controlling the development of several tissue types, notably skeletal muscle. Six1 also contributes to muscle metabolism and its activity is associated with the fast-twitch, glycolytic phenotype. Six1 regulates the expression of certain genes of the fast muscle program by directly stimulating their transcription or indirectly acting through a long non-coding RNA. We hypothesized that additional mechanisms of action of Six1 might be at play.

Methods
A combined analysis of gene expression profiling and genome-wide location analysis data was performed. Results were validated using in vivo RNA interference loss-of-function assays followed by measurement of gene expression by RT-PCR and transcriptional reporter assays.

Results
The Slc16a10 gene, encoding the thyroid hormone transmembrane transporter MCT10, was identified as a gene with a transcriptional enhancer directly bound by Six1 and requiring Six1 activity for full expression in adult mouse tibialis anterior, a predominantly fast-twitch muscle. Of the various thyroid hormone transporters, MCT10 mRNA was found to be the most abundant in skeletal muscle, and to have a stronger expression in fast-twitch compared to slow-twitch muscle groups. Loss-of-function of MCT10 in the tibialis anterior recapitulated the effect of Six1 on the expression of fast-twitch muscle genes and led to lower activity of a thyroid hormone receptor-dependent reporter gene.

Conclusions
These results shed light on the molecular mechanisms controlling the tissue expression profile of MCT10 and identify modulation of the thyroid hormone signaling pathway as an additional mechanism by which Six1 influences skeletal muscle metabolism.
Comparison of [18F] NaF PET/CT dynamic analysis methods and a static analysis method including derivation of a semi-population input function for site-specific measurements of bone formation in a population with chronic kidney disease-mineral and bone disorder
Authors: Vrist M. H. , Bech J. N. , Lauridsen T. G. , Fynbo C. A. , Theil J. 
Published: November 2021

Abstract
Purpose
The purpose of this study is to compare dynamic and static whole-body (WB) [18F]NaF PET/CT scan methods used for analysis of bone plasma clearance in patients with chronic kidney disease-mineral and bone disorder (CKD-MBD).

Methods
Seventeen patients with CKD-MBD underwent a 60-min dynamic scan followed by a 30-min static WB scan. Tracer kinetics in four thoracic vertebrae were analysed using nonlinear regression and Patlak analysis using image-derived arterial input functions. The static WB scan was analysed using a simplified Patlak method requiring only a single data point in combination with a fixed y-intercept value (V0), both obtained using a semi-population function. The semi-population function was constructed by combining a previously derived population input function in combination with data from venous blood samples. Static WB scan analysis data, obtained from the semi-population input functions, was compared with paired data obtained using dynamic input functions.

Results
Bone plasma clearance (Ki) from Patlak analyses correlated well with nonlinear regression analysis, but Ki results using Patlak analysis were lower than Ki results using nonlinear regression analysis. However, no significant difference was found between Ki obtained by static WB scans and Ki obtained by dynamic scans using nonlinear regression analysis (p = 0.29).

Conclusion
Bone plasma clearance measured from static WB scans correlates with clearance data measured by dynamic analysis. Static [18F]NaF PET/CT scans can be applied in future studies to measure Ki in patients with CKD-MBD, but the results should not be compared uncritically with results obtained by dynamic scan analysis.
Adult stroke survivor’s reintegration to normal living: a scoping review protocol
Authors: Ogunlana Michael Opeoluwa , Govender Pragashnie , Oyewole Olufemi Oyeleye , Nwosu Ifeoma Blessing 
Published: November 2021

Abstract
Background
To the best of our knowledge, a scoping review of the published literature investigating the determinants of adult stroke survivors’ reintegration to normal living has not been conducted. This scoping review aims to critically review the evidence investigating reintegration to normal living following a stroke. The following questions on reintegration to normal living after stroke will pivot this review: (i) what factors are associated with returning to normal living of stroke survivors? (ii) what are the overall determinants of reintegration to normal living of stroke survivors? To fully understand these questions, we also ask, how is reintegration to normal living assessed throughout stroke literature?

Methods
A scoping review will be conducted based on the methodology presented by Arksey and O’Malley and extended by Levac and colleagues. The Preferred Reporting Items for Systematic Review and Meta-Analysis extension for scoping reviews (PRISMA-ScR) was adopted to develop the protocol. This study will include studies involving participants ≥ 18 years old, who are stroke survivors reintegrating to normal living in the community. With no time limitations, English language publications and all study designs reporting on reintegration to normal living of stroke survivors’ will be sourced. The abstract and full-text screening will be conducted by two independent reviewers, including data charting. Thematic analysis will be used to align relevant themes and will be presented in a narrative.

Discussion
We anticipate that the scoping review will highlight the available resources and evidence on factors that determine reintegration to normal living of stroke survivors. This may contribute to informed empirical evidence for rehabilitation professionals to enhance the functional recovery of stroke survivors. It may also reveal other areas for research into reintegration to normal living for stroke survivors.

Scoping review registration
The protocol has been registered prospectively on the Open Science Framework (https://osf.io/36tuz/).
Feasibility-based fixed point networks
Authors: Heaton Howard , Wu Fung Samy , Gibali Aviv , Yin Wotao 
Published: November 2021

AbstractInverse problems consist of recovering a signal from a collection of noisy measurements. These problems can often be cast as feasibility problems; however, additional regularization is typically necessary to ensure accurate and stable recovery with respect to data perturbations. Hand-chosen analytic regularization can yield desirable theoretical guarantees, but such approaches have limited effectiveness recovering signals due to their inability to leverage large amounts of available data. To this end, this work fuses data-driven regularization and convex feasibility in a theoretically sound manner. This is accomplished using feasibility-based fixed point networks (F-FPNs). Each F-FPN defines a collection of nonexpansive operators, each of which is the composition of a projection-based operator and a data-driven regularization operator. Fixed point iteration is used to compute fixed points of these operators, and weights of the operators are tuned so that the fixed points closely represent available data. Numerical examples demonstrate performance increases by F-FPNs when compared to standard TV-based recovery methods for CT reconstruction and a comparable neural network based on algorithm unrolling. Codes are available on Github: github.com/howardheaton/feasibility_fixed_point_networks.
Complementary feeding practices and associated factors among mothers of children aged 6-23 months in Ethiopia: Secondary data analysis of Ethiopian mini demographic and health survey 2019
Authors: Shagaro Sewunet Sako , Mulugeta Be’emnet Tekabe , Kale Temesgen Dileba 
Published: November 2021

AbstractBackgroundOptimal nutrition in early child’s life plays a vital role in improving mental and motor development, reduces the possibility of contracting various infectious diseases and related deaths, decreases the risk of obesity, and fosters better overall development. However, 45% of deaths in children under five years of age that occur globally is attributed to nutrition-related factors and the majority of these deaths occur in low-and middle-income countries. Therefore, this study aims to assess complementary feeding practices and associated factors among mothers of children aged 6–23 months in Ethiopia.MethodThe study used the Ethiopian mini demographic and health survey 2019 data. A two-stage stratified cluster sampling technique was used to select 1465 mothers of children aged 6–23 months in Ethiopia. Two-level multilevel mixed-effects logistic regression model analysis was computed, and variables withp-value of less than 5% and an adjusted odds ratio with a 95% confidence interval in the final model were reported as statistically significant factors with appropriate complementary feeding practice.ResultThe overall prevalence of appropriate complementary feeding practice among mothers of children aged 6–23 months was 9.76%. In our study, mothers who attended primary[AOR = 2.72; 95%CI: 1.47–5.01], secondary[AOR = 2.64; 95%CI: 1.18–5.92] and higher school[AOR = 5.39; 95%CI: 2.29–12.64], being from medium income household[AOR = 2.89; 95%CI: 1.41–5.92], attended 1–3 times ANC visits in index pregnancy[AOR = 0.41; 95%CI: 0.18–0.89], mothers who have 12–17 months[AOR = 1.96; 95%CI: 1.16–3.33] and 18–23 months old children[AOR = 2.61; 95%CI: 1.49–4.54], currently breastfeeding mothers[AOR = 3.69; 95%CI: 1.73–7.91], mothers from pastoralist contextual regions[AOR = 0.29; 95%CI: 0.09–0.91], and mothers who have resided in rural areas[AOR = 0.49; 95%CI: 0.25–0.97] were factors significantly associated with appropriate complementary feeding practice.ConclusionThis study showed low prevalence of appropriate complementary feeding practice. Therefore, the concerned health authorities need to strengthen the existing approaches designed for provision of nutrition education particularly targeting mothers who are unschooled, who have 6–11 months old children, live in pastoralist regions and reside in rural parts of the country, and create strategies that improve maternal job opportunities.