bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irrelevant task suppresses the N170 of automatic attention allocation to fearful faces
Authors: Dou Haoran , Liang Limei , Ma Jie , Lu Jiachen , Zhang Wenhai , Li Yang 
Published: June 2021

AbstractRecent researches have provided evidence that stimulus-driven attentional bias for threats can be modulated by top-down goals. However, it is highlight essential to indicate whether and to what extent the top-down goals can affect the early stage of attention processing and its early neural mechanism. In this study, we collected electroencephalographic data from 28 healthy volunteers with a modified spatial cueing task. The results revealed that in the irrelevant task, there was no significant difference between the reaction time (RT) of the fearful and neutral faces. In the relevant task, we found that RT of fearful faces was faster than that of neutral faces in the valid cue condition, whereas the RT of fearful faces was slower than that of neutral faces in the invalid cue condition. The N170 component in our study showed a similar result compared with RT. Specifically, we noted that in the relevant task, fearful faces in the cue position of the target evoked a larger N170 amplitude than neutral faces, whereas this effect was suppressed in the irrelevant task. These results suggest that the irrelevant task may inhibit the early attention allocation to the fearful faces. Furthermore, the top-down goals can modulate the early attentional bias for threatening facial expressions.
Huge magnetoresistance in topological insulator spin-valves at room temperature
Authors: Tseng Peng , Chen Jyun-Wei , Hsueh Wen-Jeng 
Published: June 2021

AbstractTopological insulators (TI) have extremely high potential in spintronic applications. Here, a topological insulators thin-film (TITF) spin valve with the use of the segment gate-controlled potential exhibits a huge magnetoresistance (MR) value higher than 1000% at room temperature which is more than 50 times the MR of typical topological insulators (TI) spin-valves. A high spin-polarized current is provided by the band structure generated by the tunable segment potential. The results reveal a very large resistance difference between the parallel and antiparallel configurations. The MR effect is strongly influenced by the thin-film thickness, the gate potential, the gate size, and the distribution. The proposed results will help to not only improve the room-temperature performance of the spin-valves but also enhance the applications of magnetic memories and spintronic devices.
A mouse monoclonal antibody against influenza C virus attenuates acetaminophen-induced liver injury in mice
Authors: Sasaki Yutaka , Yoshino Naoto , Okuwa Takako , Odagiri Takashi , Satoh Takashi , Muraki Yasushi 
Published: June 2021

AbstractMolecular mimicry is one of the main processes for producing autoantibodies during infections. Although some autoantibodies are associated with autoimmune diseases, the functions of many autoantibodies remain unknown. Previously, we reported that S16, a mouse (BALB/c) monoclonal antibody against the hemagglutinin-esterase fusion glycoprotein of influenza C virus, recognizes host proteins in some species of animals, but we could not succeed in identifying the proteins. In the present study, we found that S16 cross-reacted with acetyl-CoA acyltransferase 2 (ACAA2), which is expressed in the livers of BALB/c mice. ACAA2 was released into the serum after acetaminophen (APAP) administration, and its serum level correlated with serum alanine aminotransferase (ALT) activity. Furthermore, we observed that S16 injected into mice with APAP-induced hepatic injury prompted the formation of an immune complex between S16 and ACAA2 in the serum. The levels of serum ALT (p < 0.01) and necrotic areas in the liver (p < 0.01) were reduced in the S16-injected mice. These results suggest that S16 may have a mitigation function in response to APAP-induced hepatotoxicity. This study shows the therapeutic function of an autoantibody and suggests that an antibody against extracellular ACAA2 might be a candidate for treating APAP-induced hepatic injury.
Quantitative analysis of in-vivo microbubble distribution in the human brain
Authors: Prada Francesco , Gennari Antonio G. , Linville Ian M. , Mutersbaugh Michael E. , Chen Zhihang , Sheybani Natasha , DiMeco Francesco , Padilla Frederic , Hossack John A. 
Published: June 2021

AbstractMicrobubbles (MB) are widely used as contrast agents to perform contrast-enhanced ultrasound (CEUS) imaging and as acoustic amplifiers of mechanical bioeffects incited by therapeutic-level ultrasound. The distribution of MBs in the brain is not yet fully understood, thereby limiting intra-operative CEUS guidance or MB-based FUS treatments. In this paper we describe a robust platform for quantification of MB distribution in the human brain, allowing to quantitatively discriminate between tumoral and normal brain tissues and we provide new information regarding real-time cerebral MBs distribution. Intraoperative CEUS imaging was performed during surgical tumor resection using an ultrasound machine (MyLab Twice, Esaote, Italy) equipped with a multifrequency (3–11 MHz) linear array probe (LA332) and a specific low mechanical index (MI < 0.4) CEUS algorithm (CnTi, Esaote, Italy; section thickness, 0.245 cm) for non-destructive continuous MBs imaging. CEUS acquisition is started by enabling the CnTI PEN-M algorithm automatically setting the MI at 0.4 with a center frequency of 2.94 MHz–10 Hz frame rate at 80 mm—allowing for continuous non-destructive MBs imaging. 19 ultrasound image sets of adequate length were selected and retrospectively analyzed using a custom image processing software for quantitative analysis of echo power. Regions of interest (ROIs) were drawn on key structures (artery–tumor–white matter) by a blinded neurosurgeon, following which peak enhancement and time intensity curves (TICs) were quantified. CEUS images revealed clear qualitative differences in MB distribution: arteries showed the earliest and highest enhancement among all structures, followed by tumor and white matter regions, respectively. The custom software built for quantitative analysis effectively captured these differences. Quantified peak intensities showed regions containing artery, tumor or white matter structures having an average MB intensity of 0.584, 0.436 and 0.175 units, respectively. Moreover, the normalized area under TICs revealed the time of flight for MB to be significantly lower in brain tissue as compared with tumor tissue. Significant heterogeneities in TICs were also observed within different regions of the same brain lesion. In this study, we provide the most comprehensive strategy for accurate quantitative analysis of MBs distribution in the human brain by means of CEUS intraoperative imaging. Furthermore our results demonstrate that CEUS imaging quantitative analysis enables discernment between different types of brain tumors as well as regions and structures within the brain. Similar considerations will be important for the planning and implementation of MB-based imaging or treatments in the future.
Anti-demineralizing protective effects on enamel identified in experimental and commercial restorative materials with functional fillers
Authors: Par Matej , Gubler Andrea , Attin Thomas , Tarle Zrinka , Tauböck Tobias T. 
Published: June 2021

AbstractThe aim of this study was to investigate whether experimental and commercial dental restorative materials with functional fillers can exert a protective anti-demineralizing effect on enamel that is not immediately adjacent to the restoration. Four experimental resin composites with bioactive glass and three commercial restorative materials were investigated. Enamel blocks were incubated in a lactic acid solution (pH = 4.0) at a standardized distance (5 mm) from cured specimens of restorative materials. The lactic acid solution was replenished every 4 days up to a total of 32 days. Surfaces of enamel blocks were periodically evaluated by Knoop microhardness measurements and scanning electron microscopy. The protective effect of restorative materials against acid was identified as enamel microhardness remaining unchanged for a certain number of 4-day acid addition cycles. Additionally, the pH of the immersion medium was measured. While enamel microhardness in the control group was maintained for 1 acid addition cycle (4 days), restorative materials postponed enamel softening for 2–5 cycles (8–20 days). The materials capable of exerting a stronger alkalizing effect provided longer-lasting enamel protection. The protective and alkalizing effects of experimental composites improved with higher amounts of bioactive glass and were better for conventional bioactive glass 45S5 compared to a fluoride-containing bioactive glass. Scanning electron micrographs evidenced the protective effect of restorative materials by showing a delayed appearance of an etching pattern on the enamel surface. A remotely-acting anti-demineralizing protective effect on enamel was identified in experimental composites functionalized with two types of bioactive glass, as well as in three commercial ion-releasing restorative materials.
Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9
Authors: Jarrell Justin A. , Sytsma Brandon J. , Wilson Leah H. , Pan Fong L. , Lau Katherine H. W. J. , Kirby Giles T. S. , Lievano Adrian A. , Pawell Ryan S. 
Published: June 2021

AbstractMicrofluidic vortex shedding (µVS) can rapidly deliver mRNA to T cells with high yield and minimal perturbation of the cell state. The mechanistic underpinning of µVS intracellular delivery remains undefined and µVS-Cas9 genome editing requires further studies. Herein, we evaluated a series of µVS devices containing splitter plates to attenuate vortex shedding and understand the contribution of computed force and frequency on efficiency and viability. We then selected a µVS design to knockout the expression of the endogenous T cell receptor in primary human T cells via delivery of Cas9 ribonucleoprotein (RNP) with and without brief exposure to an electric field (eµVS). µVS alone resulted in an equivalent yield of genome-edited T cells relative to electroporation with improved cell quality. A 1.8-fold increase in editing efficiency was demonstrated with eµVS with negligible impact on cell viability. Herein, we demonstrate efficient processing of 5 × 106 cells suspend in 100 µl of cGMP OptiMEM in under 5 s, with the capacity of a single device to process between 106 to 108 in 1 to 30 s. Cumulatively, these results demonstrate the rapid and robust utility of µVS and eµVS for genome editing human primary T cells with Cas9 RNPs.
Evaluation of hydrophobically associating cationic starch-based flocculants in sludge dewatering
Authors: Hu Pan , Shen Shaohang , Yang Hu 
Published: June 2021

AbstractTwo series of binary graft cationic starch-based flocculants (CS-DMCs and CS-DMLs) with different hydrophilicity and charge density (CD) were prepared by graft copolymerization of acrylamide with 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride and methacrylic acid 2-(benzyldimethylaminio) ethyl chloride, respectively, on the starch (St) backbone. The sludge dewatering performance of CS-DMCs and CS-DMLs were evaluated and compared based on the changes in filter cake moisture content (FCMC), specific resistance of filtration (SRF), fractions and components of extracellular polymeric substances, and various physiochemical characteristics of sludge flocs and cakes. Increase in CD of the St-based flocculants caused improved sludge dewaterability. Under the similar CD, CS-DML with relatively high hydrophobicity exhibited lower FCMC and SRF, larger and denser sludge flocs, and better permeability of sludge cakes than CS-DMCs due to the synergistic effects of charge neutralization, bridging flocculation and hydrophobic association. Furthermore, a second-order polynomial model on the basis of phenomenological theory was successfully applied to quantitatively evaluate the influences of the two important structural factors of these St-based flocculants, i.e., hydrophobicity and CD, on the sludge dewaterability. The structure–activity relationship of the St-based flocculants in sludge dewatering was obtained according to the theoretic simulation. The dewatering mechanisms was discussed in depth on the basis of the experimental and simulated results; besides, the FCMC and optimal dose can be predicted by the established structure–activity relationship. This current work offered a novel and valuable way to exploit and design of low-cost and high-performance graft natural polymeric flocculants applied in efficient conditioning of sludge.
Microbial dysbiosis reflects disease resistance in diverse coral species
Authors: MacKnight Nicholas J. , Cobleigh Kathryn , Lasseigne Danielle , Chaves-Fonnegra Andia , Gutting Alexandra , Dimos Bradford , Antoine Jendahye , Fuess Lauren , Ricci Contessa , Butler Caleb , Muller Erinn M. , Mydlarz Laura D. , Brandt Marilyn 
Published: June 2021

AbstractDisease outbreaks have caused significant declines of keystone coral species. While forecasting disease outbreaks based on environmental factors has progressed, we still lack a comparative understanding of susceptibility among coral species that would help predict disease impacts on coral communities. The present study compared the phenotypic and microbial responses of seven Caribbean coral species with diverse life-history strategies after exposure to white plague disease. Disease incidence and lesion progression rates were evaluated over a seven-day exposure. Coral microbiomes were sampled after lesion appearance or at the end of the experiment if no disease signs appeared. A spectrum of disease susceptibility was observed among the coral species that corresponded to microbial dysbiosis. This dysbiosis promotes greater disease susceptiblity in coral perhaps through different tolerant thresholds for change in the microbiome. The different disease susceptibility can affect coral’s ecological function and ultimately shape reef ecosystems.
Loss of Gap Junction Delta-2 (GJD2) gene orthologs leads to refractive error in zebrafish
Authors: Quint Wim H. , Tadema Kirke C. D. , de Vrieze Erik , Lukowicz Rachel M. , Broekman Sanne , Winkelman Beerend H. J. , Hoevenaars Melanie , de Gruiter H. Martijn , van Wijk Erwin , Schaeffel Frank , Meester-Smoor Magda , Miller Adam C. , Willemsen Rob , Klaver Caroline C. W. , Iglesias Adriana I. 
Published: June 2021

AbstractMyopia is the most common developmental disorder of juvenile eyes, and it has become an increasing cause of severe visual impairment. TheGJD2locus has been consistently associated with myopia in multiple independent genome-wide association studies. However, despite the strong genetic evidence, little is known about the functional role ofGJD2in refractive error development. Here, we find that depletion ofgjd2a(Cx35.5) orgjd2b(Cx35.1) orthologs in zebrafish, cause changes in the biometry and refractive status of the eye. Our immunohistological and scRNA sequencing studies show that Cx35.5 (gjd2a) is a retinal connexin and its depletion leads to hyperopia and electrophysiological changes in the retina. These findings support a role for Cx35.5 (gjd2a) in the regulation of ocular biometry. Cx35.1 (gjd2b) has previously been identified in the retina, however, we found an additional lenticular role. Lack of Cx35.1 (gjd2b) led to a nuclear cataract that triggered axial elongation. Our results provide functional evidence of a link betweengjd2and refractive error.
A multiscale 3D chemotaxis assay reveals bacterial navigation mechanisms
Authors: Grognot Marianne , Taute Katja M. 
Published: June 2021

AbstractHow motile bacteria navigate environmental chemical gradients has implications ranging from health to climate science, but the underlying behavioral mechanisms are unknown for most species. The well-studied navigation strategy of Escherichia coli forms a powerful paradigm that is widely assumed to translate to other bacterial species. This assumption is rarely tested because of a lack of techniques capable of bridging scales from individual navigation behavior to the resulting population-level chemotactic performance. Here, we present such a multiscale 3D chemotaxis assay by combining high-throughput 3D bacterial tracking with microfluidically created chemical gradients. Large datasets of 3D trajectories yield the statistical power required to assess chemotactic performance at the population level, while simultaneously resolving the underlying 3D navigation behavior for every individual. We demonstrate that surface effects confound typical 2D chemotaxis assays, and reveal that, contrary to previous reports, Caulobacter crescentus breaks with the E. coli paradigm.
Volatile organic compound patterns predict fungal trophic mode and lifestyle
Authors: Guo Yuan , Jud Werner , Weikl Fabian , Ghirardo Andrea , Junker Robert R. , Polle Andrea , Benz J. Philipp , Pritsch Karin , Schnitzler Jörg-Peter , Rosenkranz Maaria 
Published: June 2021

AbstractFungi produce a wide variety of volatile organic compounds (VOCs), which play central roles in the initiation and regulation of fungal interactions. Here we introduce a global overview of fungal VOC patterns and chemical diversity across phylogenetic clades and trophic modes. The analysis is based on measurements of comprehensive VOC profiles of forty-three fungal species. Our data show that the VOC patterns can describe the phyla and the trophic mode of fungi. We show different levels of phenotypic integration (PI) for different chemical classes of VOCs within distinct functional guilds. Further computational analyses reveal that distinct VOC patterns can predict trophic modes, (non)symbiotic lifestyle, substrate-use and host-type of fungi. Thus, depending on trophic mode, either individual VOCs or more complex VOC patterns (i.e., chemical communication displays) may be ecologically important. Present results stress the ecological importance of VOCs and serve as prerequisite for more comprehensive VOCs-involving ecological studies.
Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins
Authors: McKitrick Tanya R. , Bernard Steffen M. , Noll Alexander J. , Collins Bernard C. , Goth Christoffer K. , McQuillan Alyssa M. , Heimburg-Molinaro Jamie , Herrin Brantley R. , Wilson Ian A. , Cooper Max D. , Cummings Richard D. 
Published: June 2021

AbstractThe terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification. To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression. O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays. 3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase. O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3). The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.
Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
Authors: Soboleva Svetlana , Kurita Ryo , Ek Fredrik , Åkerstrand Hugo , Silvério-Alves Rita , Olsson Roger , Nakamura Yukio , Miharada Kenichi 
Published: June 2021

AbstractImmortalized erythroid cell lines are expected to be a promising source of ex vivo manufactured red blood cells (RBCs), however the induction of enucleation in these cell lines is inefficient at present. We utilized an imaging-based high-throughput system to identify chemical compounds that trigger enucleation of human erythroid cell lines. Among >3,300 compounds, we identified multiple histone deacetylase inhibitors (HDACi) inducing enucleated cells from the cell line, although an increase in membrane fragility of enucleated cells was observed. Gene expression profiling revealed that HDACi treatment increased the expression of cytoskeletal genes, while an erythroid-specific cell membrane protein, SPTA1, was significantly down-regulated. Restoration of SPTA1 expression using CRISPR-activation partially rescued the fragility of cells and thereby improved the enucleation efficiency. Our observations provide a potential solution for the generation of mature cells from erythroid cell lines, contributing to the future realization of the use of immortalized cell lines for transfusion therapies.
SARS-CoV-2 proteins and anti-COVID-19 drugs induce lytic reactivation of an oncogenic virus
Authors: Chen Jungang , Dai Lu , Barrett Lindsey , James Jennifer , Plaisance-Bonstaff Karlie , Post Steven R. , Qin Zhiqiang 
Published: June 2021

AbstractAn outbreak of the novel coronavirus SARS-CoV-2, the causative agent of Coronavirus Disease-2019 (COVID-19), a respiratory disease, has infected almost one hundred million people since the end of 2019, killed over two million, and caused worldwide social and economic disruption. Because the mechanisms of SARS-CoV-2 infection of host cells and its pathogenesis remain largely unclear, there are currently no antiviral drugs with proven efficacy. Besides severe respiratory and systematic symptoms, several comorbidities increase risk of fatal disease outcome. Therefore, it is required to investigate the impacts of COVID-19 on pre-existing diseases of patients, such as cancer and other infectious diseases. In the current study, we report that SARS-CoV-2 encoded proteins and some currently used anti-COVID-19 drugs are able to induce lytic reactivation of Kaposi’s sarcoma-associated herpesvirus (KSHV), one of major human oncogenic viruses, through manipulation of intracellular signaling pathways. Our data indicate that those KSHV + patients especially in endemic areas exposure to COVID-19 or undergoing the treatment may have increased risks to develop virus-associated cancers, even after they have fully recovered from COVID-19.
Single-nucleotide conservation state annotation of the SARS-CoV-2 genome
Authors: Kwon Soo Bin , Ernst Jason 
Published: June 2021

AbstractGiven the global impact and severity of COVID-19, there is a pressing need for a better understanding of the SARS-CoV-2 genome and mutations. Multi-strain sequence alignments of coronaviruses (CoV) provide important information for interpreting the genome and its variation. We apply a comparative genomics method, ConsHMM, to the multi-strain alignments of CoV to annotate every base of the SARS-CoV-2 genome with conservation states based on sequence alignment patterns among CoV. The learned conservation states show distinct enrichment patterns for genes, protein domains, and other regions of interest. Certain states are strongly enriched or depleted of SARS-CoV-2 mutations, which can be used to predict potentially consequential mutations. We expect the conservation states to be a resource for interpreting the SARS-CoV-2 genome and mutations.