bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complication Rates of Percutaneous Brachial Artery Puncture: Effect of Live Ultrasound Guidance
Authors: Appelt Konrad , Takes Martin , Zech Christoph J , Schubert Tilman 
Published: August 2021

Abstract
PurposeThe current literature on the use of brachial artery access is controversial. Some studies found increased puncture site complications. Others found no higher complication rates than in patients with femoral or radial access. The purpose of this study was to determine the impact of ultrasound (US)-guidance on access site complications.Materials and MethodsThis is a single-center retrospective study of all consecutive patients with brachial arterial access for interventional procedures. Complications were classified into minor complications (conservative treatment only) and major complications (requiring surgical intervention). The brachial artery was cannulated in the antecubital fossa under US-guidance. After the intervention, manual compression or closure devices, both followed by a compression bandage for 3 hours, either achieved hemostasis.Results75 procedures in 71 patients were performed in the study period using brachial access. Access was successful in all cases (100%). Procedures in different vascular territories were performed: neurovascular (11/14.7%), upper extremity (36/48%), visceral (20/26.7%), and lower extremity (12/16%). Sheath size ranged from 3.2F to 8F (mean: 5F). Closure devices were used in 17 cases (22.7%). In total, six complications were observed (8.0%), four minor complications (5.3%, mostly puncture site hematomas), and two major complications, that needed surgical treatment (2.7%). No brachial artery thrombosis or upper extremity ischemia occurred.ConclusionExclusive use of US-guidance resulted in a low risk of brachial artery access site complications in our study compared to the literature. US-guidance has been proven to reduce the risk of access site complications in several studies in femoral access. In addition, brachial artery access yields a high technical success rate and requires no additional injection of spasmolytic medication. Sheath size was the single significant predictor for complications.
Optimized Channel Decision &amp; Secure Data Transmission for Health Monitoring Using Wireless Body Area Network Through Cognitive Controller
Authors: Rajaguru R. , Devi K. Vimala , Marichamy P. 
Published: August 2021

Abstract
Remote monitoring system has been applied in different applications such as agriculture, industrial automation, defence, telecommunication and health care. In health care applications, wireless networks get the impact with Wireless Body Area Network (WBAN). WBAN is helpful in monitoring patients’ health and it also possesses secure transmission and access control mechanism with different sensors. WBAN monitors the patient’s health and transfers the information to data pool without influencing patient’s daily routine activities. Further, the health report data are sent to the doctor over the network from the place of the patient without any data loss and delay. Due to increasing usage of wireless services, the available networks have been congested with heavy traffic which leads to miscommunication and delay. To overcome this scenario, solution has been proposed with help of Cognitive Radio Networks (CRN). Collected information are transferred to cognitive controller which acts as central node. Cognitive controller selects the channel to transfer the information with QoS as well as without any delay. Based on the input parameters, the channel selection process is optimized and it will also improve the system performance with secure transmissions. Using Fuzzy Inference System (FIS) optimizing, the channel selection process has been carried out and it also provides more accurate solution to choose the channel. For the optimization of the proposed approach Mamdani and Sugeno methods have been used. These methods yield the best results with minimum error probability of 0.9 compared to the existing methods and these methods have achieved efficiencies of 98% and 99%, respectively.
Occurrence and health risk assessment of perfluorinated substances from water in residential areas around fluorine chemical industry, China
Authors: Tang Jiaxi , Zhu Yongle , Li Yu , Xiang Biao , Tan Ting , Lv Linyou , Luo Qing 
Published: August 2021

Abstract
To identify the contamination status and assess the health risk of per- and polyfluoroalkyl substances (PFASs) in surface and ground water are of great significance recently. Eighteen individual PFASs were analyzed in thirty-three surface/ground water samples from fluorine industry in one period of park-A (park A) and two periods of park B. All the short chain PFASs (C < 8) and chlorinated polyfluorinated ether sulfonate acid (F-53B) in the park A and B were detected with 100%. The first three detected substances of perfluorobutane sulfonate (PFBS), perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOA) were the predominant substances. The contamination status in wet season (WS) is stronger than in dry season (DS) in park B. Total concentration of PFASs(∑PFASs) in park A presented the increasing tendency following the groundwater flow direction whereas this rule was limited to reflect in all periods of park B. Applying different relative source contribution (RSC) of 100% and 20% to assess this risk of different aged group bodies from PFASs, the result was that all PFASs (4 ≤ C ≤ 7 or 9 ≤ C ≤ 12) while perfluorooctane sulfonate (PFOS) and PFOA (C = 8) were identified to the low risk quotient (RQ) contribution. Mixed RQmix value mainly relies on the PFOA and PFOS with a larger contribution rate of 80–90%. All assessed cases (Case 1, Case2, Case3 and Case4) to all aged groups reveal the infants were to be vulnerable to PFASs influence, followed by the children, teenagers and adults, respectively.
Effect of Transdermal Microneedle Patch with NSAID in Osteoarthritic Knee: A Randomized Controlled Trial
Authors: Khuangsirikul Saradej , pisuttanawat Mongkon , Heebthamai Danai , Khanchaitit Paisan , Chotanaphuti Thanainit 
Published: August 2021

Abstract
Background: In early osteoarthritis (OA) knee without structural damage, synovial hypertrophy is a good biomarker to monitor disease progression and response to any treatments. NSAID has been proved to reduce inflammation or synovitis effectively. Transdermal microneedle is a non-invasive device to deliver medication by transdermal route. However, no recent study shows the effectiveness of transdermal NSAID via microneedle.Objective: To determine the effect of transdermal microneedle patch (TDM) with NSAID in synovial hypertrophy, knee pain and WOMAC score of osteoarthritic knees. Methods: Two-group, randomized, double-blinded, controlled trial was done. 100 participants, age 40-70 years, with painful knee OA and non-structural change on radiography will be randomly allocated to underwent either TDM (placebo) or TDM with NSAID (ketorolac 30 mg) at medial joint line of the knee once weekly for 2 times. Synovial thickness was measured by ultrasonography at pre-treatment, 1, 2, and 4 weeks. Pain score (VAS), WOMAC score and adverse events (AEs) were also recorded.Results: One hundred patients were enrolled into 2 groups. Comparing to TDM with NSAID and Placebo group demonstrated significant reduction of synovial thickness and VAS at 2, 4 weeks (P<0.05). Mean synovial thickness reduction were 1.12 and 0.27 mm. at 4 weeks and mean VAS reduction were 3.22 and 1.7 at 4 weeks (TDM with NSAID and placebo, respectively), but mean WOMAC score reduction statistically significant difference between groups at 4th weeks. Mean WOMAC score reductions were 5.71 and 0.96 at 4 weeks (TDM with NSAID and placebo, respectively). Treatment related adverse events in total 4 weeks, i.e. skin irritation, injection site pain, superficial skin infection were similar in all groups and no report of any complicationConclusion: Transdermal microneedle patch with NSAID significantly decreased synovitis and improved pain score in OA knee after 2 weeks and improve WOMAC score over 4th week without any adverse events. Trial registration: We enroll 100 the primary OA knee patients for their willingness to participate in this study. This research was enrolled on 22 April 2020, Research Ethic committee approved on 21 April 2020. During October, 22 2020 research were completed.
Pharmacokinetics of the Couple of Radix Aconiti Lateralis Preparata and Cinnamomum Cassia in Rats with Osteoporosis with Kidney-yang Deficiency by UPLC and Microdialysis Method
Authors: Meng Yuxiao , Guan Jiaqi , Yao Liping , Li Xiaojun , Qin Luping , Zhang Lu 
Published: August 2021

Abstract
Background: To confirm the metabolites of the Aconite and Cinnamon herb couple in rat, and characterize its pharmacokinetics.Methods: Microdialysis probes were inserted into the jugular vein and knee joint of Sprague Dawley rat, and dialysates of different administration groups were collected. Target analytes were separated on a hydrophilic interaction liquid chromatography column (ACQUITY UPLC BEH C8 2.1×100 mm, 1.7 μm) and analyzed with an ultra-performance liquid chromatography (UPLC) under multiple reaction monitoring modes.Results: The experiment shows that the concentrations of quality of the three metabolites in mixing extraction of the herb couple were 0.3701% for trans-cinnamic acid, 0.1249 % for mesaconitine, and 0.0469 % for hypoaconitine, and Cinnamon group was 0.1731 % for trans-cinnamic acid, Aconite group were 0.0017 % for mesaconitine, and 0.0300 % for hypoaconitine. The concentrations of quality of the metabolites in rat plasma of the herb couple group were 0.0028% for trans-cinnamic acid, 0.0947% for mesaconitine, and 0.1124% for hypoaconitine. And the concentrations of quality of Aconite group and Cinnamon group were 0.0019% for trans-cinnamic acid, 0.0307% for mesaconitine, and 0.0220% for hypoaconitine. Pharmacokinetic results showed that the mean half-lives of the microdialysis samples of blood of Cinnamon group was 492.18 min for trans-cinnamic acid, and Aconite group were 102.48 min for mesaconitine and 93.27 min for hypoaconitine, and the herb couple ware 181.36 min for trans-cinnamic acid, 103.9 min for mesaconitine and 116.01 min for hypoaconitine, and the microdialysis samples of joint of Cinnamon group was 190.85 min for trans-cinnamic acid, and Aconite group were 48.51 min for mesaconitine and 46.01 min for hypoaconitine, and the herb couple was 131.19 min for trans-cinnamic acid, 49.36 min for mesaconitine and 146.68 min for hypoaconitine.Conclusions: The mixed extraction of aconite and cinnamon can promote the dissolution of the active ingredients. The herb couple can promote the absorption of the active ingredients, improve the distribution of the active ingredients in the joint and blood, prolong the half-lives of the active ingredients. It shows that the compatibility of aconite and cinnamon can increase the bioavailability of the drug and improve the clinical efficacy.
Altered Spontaneous Brain Activity Patterns in Patients with Age-related Macular Degeneration: A Resting-State fMRI Study Running head: Functional Connectivity Density alterations in age-related macular degeneration patients
Authors: Huang Rong , Zhang Li-Juan , Chen Min-Jie , Ge Qian-Min , Shu Hui-Ye , Pan Yi-Cong , Liang Rong-Bin , Li Qiu-Yu , Shao Yi 
Published: August 2021

Abstract
Background

Many neuroimaging studies have confirmed that age-related macular degeneration (AMD) is closely related to the changes of brain functional areas. However, the value of spontaneous brain activity changes induced by AMD in early diagnosis of diseases has not been confirmed.
Purpose

To explore potential functional network brain activity in patients with AMD using the voxel-wise degree centrality (DC) method and reveal the functional changes of specific brain regions in patients with AMD.
Material and Methods

Eighteen patients with AMD (10 males, 8 females) and eighteen healthy controls (HCs) (10 males, 8 females) matched by gender, age, and education level were enrolled. The resting-state fMRI scanning was applied to measure changes in initiative brain activities. We displayed the differences between the AMD group and the healthy group with the receiver operating characteristic curves. Using correlation analysis, the average DC values in specific cerebrum regions were analyzed to explore changes of behavioral performance in AMD patients. The Hamilton Rating Scale for Depression and Anxiety (Ham-D/A) was used to evaluate the relationship between emotional states and DC values of the subcallosal gyrus in AMD patients.
Results

In AMD patients, DC values were significantly lower in the bilateral lingual gyrus but were higher in the bilateral inferior temporal gyrus, bilateral subcallosal gyrus, bilateral fusiform gyrus, bilateral middle frontal gyrus, and left orbital middle frontal gyrus/insular gyrus/inferior frontal gyrus. There was no direct relationship between the behavioral performance and the average DC values of some areas.
Conclusion

The DC values changed with the fluctuation of spontaneous brain activities in AMD patients, which may be beneficial for clinical diagnosis.
Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairments
Authors: Zhang Manman , Gong Wenliang , Chen Beina , Ji Ming , Chen Binjie , Zhang Dianjun , Li Xinyu , Zhou Yuefei , Dong Chengyi , Wen Gehua , Zhan Xiaoni , Wu Xiafang , Xia Maosheng , Li Baoman , Verkhratsky Alexei 
Published: August 2021

Abstract

Alzheimer's disease (AD) is the prevalent cause of dementia in the ageing world population. Apolipoprotein E4 (ApoE4) allele is the key genetic risk factor for AD, although the mechanisms linking APOE4 with neurocognitive impairments and aberrant metabolism remains to be fully characterised. We discovered significantly increase in the ApoE4 contents of serum exosomes in old healthy subjects and AD patients carrying ApoE4 allele as compared with adult healthy subjects. Elevated exosomal ApoE4 demonstrated significant inverse correlation with serum thyroid hormones level and cognitive function. We analysed effects of ApoE4 in peripheral exosomes on the neurological disorders in aged or thyroidectomied young mice. Ageing-associated hypothyroidism as well as acute thyreoidectomy augmented transport of liver-derived ApoE4 reach exosomes into the brain, where ApoE4 activates NLRP3 inflammasome by increasing cholesterol level in neural cells. This, in turn, affects cognition, locomotion and mood. Our study reveals pathological potential of exosomes-mediated relocation of ApoE4 from the periphery to the brain, which process can represent potential therapeutic target.
Robust 3D modelling reveals spatiosyntenic properties of animal genomes
Authors: Clarence Tereza , Robert Nicolas , Sarigol Fatih , Fu Xiao , Bates Paul , Simakov Oleg 
Published: August 2021

Abstract
Animal genomes are organized into chromosomes that are remarkably conserved in their gene content, forming distinct evolutionary units (macrosynteny). We developed a novel three-dimensional chromosomal modelling approach to show that syntenic signals are reflected in conserved three-dimensional networks, encompassed within interaction spheres. We show evidence for evolutionary constraints that could not be surmised by genomic sequence alone, thereby underlining the importance of three-, rather than just two-, dimensional organization.
Trend analysis of seasonal rainfall and temperature pattern in Damota Gale districts of Wolaita Zone, Ethiopia
Authors: Bojago Elias , Yaya Dalga 
Published: August 2021

Abstract
Background: This paper investigated the recent trends of precipitation and temperature on Damota Gale districts of Wolaita Zone. This study used the observed historical meteorological data from 1987 to 2019 to analyze the trends. The magnitude of the variability or fluctuations of the factors varies according to locations. Hence, examining the spatiotemporal dynamics of meteorological variables in the context of changing climate, particularly in countries where rain fed agriculture is predominant, is vital to assess climate-induced changes and suggest feasible adaptation strategies. Results: Both rainfall and temperature data for period of 1987 to 2019 were analyzed in this study. Statistical trend analysis techniques namely Mann–Kendall test and Sen's slope estimator were used to examine and analyze the problems. The long-term trend of rainfall and temperature was evaluated by linear regression and Mann–Kendall test. The temperature was shown a positive trend for the both annual and seasonal periods and had a statistical significance at 95%.Conclusion: This study concluded that there were a declining rainfall in the three seasons; spring, summer and winter but in autumn it shows increasing trends and rapid warming, especially in the last 32 years. The detailed analysis of the data for 32 years indicate that the annual maximum temperature and annual minimum temperature have shown an increasing trend, whereas the Damota Gale seasonal maximum and minimum temperatures have shown an increasing trend. The findings of this study will serve as a reference for climate researchers, policy and decision makers.
ctDNA in Breast Milk for Early Detection of Pregnancy Associated Breast Cancer
Authors: Saura Cristina , Ortiz Carolina , Lahuerta Enrique Arenas , Matito Judit , Suñol Anna , Córdoba Octavi , Aliguer Alex Martínez-Sabadell , García-Ruiz Itziar , Miranda Ignacio , Morales-Comas Clara , Carrasco Estela , Viaplana Cristina , Peg Vicente , Nuciforo Paolo , Bayó-Puxan Neus , Miquel Josep , Gómez-Rey Marina , Villacampa Guillermo , Arévalo Silvia , Espinosa-Bravo Martin , Balmaña Judith , Dienstmann Rodrigo , Arribas Joaquín , Tabernero Josep , Sanso Miriam , Vivancos Ana 
Published: August 2021

Abstract
Pregnancy associated breast cancer (PABC) is an overall poor prognosis group compared to non-PABC breast cancer (BC), mainly due to late diagnosis. Effective screening approaches for early detection may improve its outcome. For the first time, we demonstrate the presence of cell-free tumor DNA (ctDNA) in the breast milk serum (sBM) of PABC in patients with early-stage disease and its ability to recapitulate somatic mutations present in the primary tumor. Moreover, our results demonstrate that sBM robustly surpasses plasma for tumor genetic profiling due to a prevalent shedding of ctDNA into the sBM in localized disease, as well as increased total cell-free DNA (cfDNA) abundance and integrity. Thus, we propose sBM as a potential new non-invasive liquid biopsy for a prompt detection of PABC.
Non-covalent interactions of Cysteine onto C60, C59Si, and C59Ge: A DFT study
Authors: Mohammadi Mohsen Doust , abdullah hewa Y 
Published: August 2021

Abstract
The study of intermolecular interactions is of great importance. This study attempted to quantitatively examine the interactions between Cysteine (C3H7NO2S) and fullerene nanocages, C60, in a vacuum. As the frequent introduction of elements as impurities into the structure of nanomaterials can increase the intensity of intermolecular interactions, nanocages doped with silicon and germanium have also been studied as adsorbents C59Si and C59Ge. Quantum mechanical studies of such systems are possible in the density functional theory (DFT) framework. For this purpose, various functionals, such as B3LYP-D3, ωB97XD, and M062X, have been used. One of the most suitable basis functionals for the systems studied in this research is 6-311G (d), which has been used in both optimization calculations and calculations related to wave function analyses. The main part of this work is the study of various analyses that reveal the nature of the intermolecular interactions between the two components introduced above. The results of conceptual DFT, natural bond orbital, non-covalent interactions, and quantum theory of atoms in molecules were consistent and favored physical adsorption in all systems. Germanium had more adsorption energy than other dopants. The HOMO–LUMO energy gaps were as follows: C60: 5.996, C59Si: 5.309, and C59Ge: 5.188 eV at B3LYP-D3/6-311G (d) model chemistry. The adsorption sensitivity increased when an amino acid molecule interacted with doped C60, and this capability could be used to design a nanocarrier to detect Cysteine amino acids.
Combination therapy with Azithromycin, Pentoxifylline and Dexamethasone shows Complete Recovery in Severe COVID 19: Case Series of Patients in First and Second wave of Pandemic in India.
Authors: Kler Rupinder , Mukherjee Anirudh , Banerjee Ms Pratiti , Kaur Gurkeerat , Vaishnav Dilip 
Published: August 2021

Abstract
Background: COVID 19 infection caused by SARS-CoV2 virus is causing high mortality all over the world. This causes asymptomatic, mildly symptomatic , moderate, and severe illness. In severe illness, mortality is remarkably high as drugs are not effective enough to avert cytokine storm, stroke, myocarditis, or myocardial infarction. COVID-19 is a positive sense virus that directly enters the ACE2 cell and starts replication after encountering the host protein. Effective treatment has been limited to only mild and sometimes moderate cases but there is a high mortality in severe cases. Case Presentation: Severe COVID-19 cases with different comorbities were assessed and treated in the first wave as well as the second wave of the pandemic in North India.. A total number of 305 patients were assessed for severity with their symptoms and abnormal blood parameters , out of which 109 patients severe patients were given the drugs and their symptoms and vitals were monitored along with therapy. In both the single SARS CoV 2 and the double mutant virus strain in the second wave. We measured their severity through their blood parameters by calculating high Neutrophil lymphocyte ratio, Platelet lymphocyte ratio after getting their complete blood count, diabetes, and other inflammatory markers like high LDH, CRP, Serum Ferritin, D Dimer, or IL 6 in critically ill patients as studied in previous studies. Hence, we designed this combination therapy drug protocol which was home based oral therapy with hundred percent recovery rate if initiated early. Wherever required oxygen therapy had also been recommended along with diet modification, low fatty acid diet and low carbohydrates for more effectivity. The combination of Azithromycin , Pentoxifylline and Dexamethasone had shown to be highly effective in controlling the inflammation and viral replication with dose modification. Intravenous Ceftriaxone was given as infusion in patients having viral Pnemonia .Conclusions: This combination therapy of drugs has shown to decrease TLR mediated cytokine production. This therapy has shown full success in all high-risk patients especially in the elderly having comorbidities like Diabetes, heart diseases, cancer, autoimmune diseases.