bluuebunny/crossref_metadata_2025_split huggingface dataset:
CIUDADES INTELIGENTES, MARCO REGULATORIO EN COLOMBIA
Authors: Ortega Sanchez  Paola Astrid , Salazar Hugo Felipe , Fernández González Herman Antonio 
Published: August 2021

En este trabajo se presenta una revisión de literatura de los aspectos regulatorios y normativos que se han venido desarrollando bajo el concepto de ciudades inteligentes, smart Cities, en Colombia. A través del Ministerio de Tecnologías de la Información y las Comunicaciones (MinTIC) se ha propuesto elaborar una política pública con el objeto de estandarizar la implementación de tecnología en los diversos sectores de la sociedad colombiana durante los próximos cinco años. En este sentido, se han sintetizado los aspectos a regular de acuerdo con los siete componentes propuestos por MinTIC: competitividad, convivencia, cultura, hábitat, infraestructura, recursos y servicios, haciendo una breve descripción del marco normativo que podría aplicarse a cada uno de los componentes en particular.
Aesop's fables adaptation: an alternative for fostering values, oral production and listening comprehension
Authors: Pedraza Hernández Blanca Ximena , Castiblanco Gil Heidy Liliana 
Published: August 2021

This article aims at presenting the experiences acquired from the project titled “Aesop’s Fables Adaptation: An Alternative for Fostering Values, Oral Production and Listening Comprehension”, carried out at a public school in Tunja with fourth graders. The project related to the adaptation of Aesop´s fables, which were included in the school´s curriculum topics and implemented through different workshops. This was done in order to develop values awareness and English skills. Throughout this project, most of the students participated, were very attentive class after class, and enjoyed the activities. The students began to use the vocabulary they learnt from the fables for communicative purposes. In the same way, students were aware of moral aspects, the implicit values in each story, and the fables’ importance in their own lives
Experimental Verification of General Validity of Non-Force (Information) Interaction Formula
Authors: Teslia Iurii 
Published: August 2021

Abstract
The work is devoted to the demonstration of the possibility of applying the formulas of information handling obtained in the theory of non-force interaction for the natural language processing. These formulas were obtained in computer experiments in modelling the movement and interaction of material objects by changing the amount of information that triggers this movement. The hypothesis, objective and tasks of the experimental research were defined. The methods and software tools were developed to conduct the experiments. To compare different results of the simulation of the processes in a human brain during speech production, there was a range of methods proposed to calculate the estimate of sequence of fragments of natural language texts including the methods based on linear approximation. The experiments confirmed that the formulas of information handling obtained in the theory of non-force interaction reflect the processes of language formation. It is shown that the offered approach can successfully be used to create systems of reactive artificial intelligence machines. Experimental and, presented in this work, practical results constitute that the non-force (informational) interaction formulae are generally valid.
Purification and Characterization of lipase enzyme from endophytic Bacillus pumilus WSS5 for application in detergent industry.
Authors: Khan Sofia Sharief , Verma vijeshwar , Rasool Shafaq 
Published: August 2021

Abstract
Lipolytic enzymes from endophytic microbes have been the focus of intense and growing research in view of the applicability of such enzymes in various industrial applications and endophytic microorganisms may emerge as a potential source of lipases with distinct characteristics. The current work involved purification and characterization of the lipase from an endophytic-bacteria isolated from the stem of Withania somnifera L. Dunal. Based on the analysis of morphology and 16S rDNA sequence, the endophytic bacteria was identified as Bacillus pumilus and designated as B. pumilus WSS5. Purified lipase from the B. pumilus WSS5 was found to be of 28 KDa as revealed by SDS-PAGE with optimum activity at 37°C and pH 8. The enzyme was found to be stable over a broad pH range of 6.0–9.0 and temperature stability was in the range of 10°C to 60°C. The enzyme activity was enhanced in the presence of organic solvents like Ethyl acetate, Methanol, 2-propanol, acetone, and ethanol. The stability of the enzyme in presence of various metal ions, surfactants, inhibitors, and kinetic parameters value suggested that this enzyme could be exploited in leather and detergent industries.In addition, the stain removal potential of the crude and purified lipase was determined on cotton fabric pieces stained with vegetable oil. Lipase added to water along with detergent significantly enhanced the stain removal efficiency of a commercially available detergent. The current findings suggest the potential of B. pumilus WSS5 lipase owing to promising properties such as the alkali-thermostability, organic solvent-tolerance, and broad substrate specificity that makes it a strong candidate for application in detergent formulations, biotransformation, industries, and medicine. To our knowledge, this is the first report on the purification and characterization of lipase from bacterial endophyte isolated from the stem of Withania somnifera L. Dunal.
Prognostic Value of Immune-Related IncRNA pairs in Patients with Bladder Cancer
Authors: Gao zhenzhen , Wu Dongjuan , Zheng Wenwen , Zhu taohong , Sun Ting , Yuan Lianhong , Fei Faming , Fu Peng 
Published: August 2021

Abstract
Background: The characteristics of immune-related long non-coding ribonucleic acids (ir-lncRNAs), regardless of their specific levels, have important implications for the prognosis of patients with bladder cancer. Methods: Based on The Cancer Genome Atlas database, original transcript data were analyzed, the ir-lncRNAs were obtained using a coexpression method, and the differentially expressed pairs of ir-lncRNAs (DE-ir-lncRNAs) were identified by univariate analysis. The lncRNA pairs were verified using a Lasso regression test. Thereafter, receiver operating characteristic curves (ROC) were generated, area under the curve was calculated, Akaike information criterion of the 5-year ROC was determined, optimal cutoff value of the high- and low-risk populations of patients with bladder cancer was confirmed, and optimal risk model was established. The clinical value of the model was verified through the analysis of patient survival rates, clinicopathological characteristics, presence of tumor-infiltrating immune cells, and chemotherapy efficacy evaluation. Results: In total, 49 pairs of DE-ir-lncRNAs were identified, of which 21 were included in the Cox regression model. A risk regression model was established on the premise of not involving the specific expression value of the transcripts.Conclusions: The method and model used in this study have important clinical predictive value for bladder cancer and other malignant tumors.
Geophysical and Geotechnical Investigation of Building Failure in A Typical Basement Complex Environment – A Case Study in Ile-Ife, Nigeria
Authors: Afolabi Olaolu , Oladeji Ayoola Temiloluwa , Salami Babatunde Moruf , Lawal Sadiq Akorede 
Published: August 2021

Abstract
A building located within the Basement Complex of the ancient town of Ile – Ife, Osun State, Southwestern Nigeria was observed to have failed due to excessive total and differential settlement. The failure was investigated using the electrical resistivity and geotechnical methods The electrical resistivity method involved the 2-D electrical resistivity imaging (ERI) technique using the dipole-dipole array along four traverses of 30 – 60 m in lengths. The geotechnical method involved the cone penetration test (CPT) using the 2.5-ton static penetrometer machine. Quantitative and qualitative analysis of the ERI data were made using the DIPROfWIN software for the pseudo-inversion while the CPT data were interpreted for lithology using standard chart. The results show that the topsoil, about 1.0 m thick, is composed of sandy clay/clay that is characterized by cone resistance (qc) of 0.2 – 2.0 MPa and resistivity of 75 - 200 Ωmm. The underlying clayey weathered layer, which constitute the shallow foundation soil is characterized by thickness of 4 - >10 m, qc of 0.2 – 1.0 MPa, resistivity of 25 - 75 Ωmm and estimated consolidation settlement of 200 – 500 mm. The basal layer is the saprock/fresh bedrock characterized by qc of > 8.0 MPa and resistivity of 100 - 1000 Ωmm. The subsoil is thus characterized by variably thick incompetent clayey weathered layer within which the shallow foundation was placed; hence the excessive total and differential settlements.
Assessment of Heavy Metal Pollution of the Snow Cover of the Severodvinsk Industrial District (Russia, the Arkhangelsk Region).
Authors: Yakovlev E. Yu. , Druzhinina A.S. , Zykova E.N. , Zykov S.B. , Ivanchenko N.L. 
Published: August 2021

Abstract
In the present work, the content of heavy metals in soluble and insoluble forms of snow was investigated, as well as in waters from wells near the Severodvinsk industrial centre (Russia, the Arkhangelsk region). The content of heavy metals (Al, Fe, Ti, Mn, Zn, Cr, Cu, Ni, Pb, Co, V, As, and Cd) in the melt snow filtrate and solid residue was measured using the ICP-MS method. On the territory of the city, there are high concentrations of metals, high values ​​of electrical conductivity and low pH values ​​in objects most susceptible to anthropogenic pollution. It was determined that in urban conditions the share of the solid-phase component increases, which indicates an increase in dust fallout. Statistical analysis showed that most of the metals in the snow cover are linked with each other by a strong correlation (r> 0.9), and also made it possible to identify sources of pollution, namely thermal power plants and shipbuilding enterprises. The calculation of various indices for assessing the snow quality and natural waters determined the level of heavy metal pollution in the Severodvinsk industrial district as extremely high and dangerous for public health. The radiation parameters of water from wells were studied and a satisfactory radioecological characterisation of the research area was established.
Physiological and Proteomic Analysis Revealed the Response Mechanisms of two Different Grought-resistant Maize Varieties
Authors: Li Hongjie , Yang Mei , Zhao Chengfeng , Wang Yifan , Zhang Renhe 
Published: August 2021

Abstract
Background: Drought stress seriously limits the seedling growth and yield of maize. Despite previous studies on drought resistance mechanisms by which maize cope with water deficient, the link between physiological and molecular variations are largely unknown. To reveal the complex regulatory mechanisms, comparative physiology and proteomic analyses were conducted to investigate the stress responses of two maize cultivars with contrasting tolerance to drought stress. Results: Physiological results showed that SD609 (drought-tolerant) maintains higher photochemical efficiency by enhancing CEF (cyclic electron flow) protective mechanism and antioxidative enzymes activities. Proteomics analysis revealed a total of 198 and 102 proteins were differentially expressed in SD609 and SD902, respectively. Further enrichment analysis indicated that drought-tolerant ‘SD609’ increased the expression of proteins related to photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones and metabolic enzymes. The up-regulation proteins related to PSII repair and photoprotection mechanisms resulted in more efficient photochemical capacity in tolerant variety under moderate drought. However, the drought-sensitive ‘SD902’ only induced molecular chaperones and sucrose synthesis pathways, and failed to protect the impaired photosystem. Further analysis indicated that proteins related to the electron transport chain, redox homeostasis and heat shock proteins (HSPs) could be important in protecting plants from drought stress. Conclusions: Our experiments explored the mechanism of drought tolerance, and obtained detailed information about the interconnection of physiological research and protein research. In summary, our findings could provide new clues into further understanding of drought tolerance mechanisms in maize.
A Comparative Study On The Physical Properties of Natural Sedimentary Loess And Manual Filling Compacted Loess
Authors: Zhang Linxin , Qi Shengwen , Yu Yongtang , Zhang Yaguo , Li Zhiqing , Hou Xiaokun , Ma Lina , Zou Yu , Guo Songfeng , Peng Jianbing 
Published: August 2021

Abstract
With the development of human society, mega engineering projects of removing the tops of hills to infill valleys began to appear in the loess region. The thickness of the manual filling compacted loess can reach tens of meters. For such large-scale construction projects, studying the properties of compacted loess is essential to ensure the safety and reliability of land creation and artificial infrastructure. In this paper, the specimens from two exploration well profiles were carried out to study the physical properties of natural loess and compacted loess from the Loess Plateau. Here the natural loess selected was deposited in old ages (Q2 and Q1) and had strong stability. The natural water content, dry density, specific gravity, liquid limit, plastic limit, plasticity index, clay fraction, silt fraction, sand fraction, compression modulus, and permeability coefficient have been determined. Statistical theories such as t-test and correlation coefficient checks were used to describe the difference between the two kinds of loess, and the degree of correlation among various indicators. Besides, 14 groups of exploration well data in 8 studies were collected. The variation of natural water content and dry density with well depth was analyzed to supplement the existing data. Results have shown that the manual filling compacted loess is significantly different from the natural loess. On the whole, the liquid limit, plastic limit, plasticity index, clay fraction, silt fraction, sand fraction and compression modulus of the compacted loess are smaller. And compared with the natural sedimentary loess with strong stability, it deforms more easily. The difference of compression modulus between the compacted loess and natural loess is mainly controlled by the dry density and the particle composition. Moreover, the heterogeneous level of the manual filling compacted loess is greater than that of the natural loess in the horizontal direction and smaller than that of the natural loess in the vertical direction. Under a combination of external hydrologic conditions and dead weight, the compacted loess will become more stable.
Assessing The Impacts of Wet Treatment On Leaching Toxicity of Municipal Solid Waste Incineration Bottom Ash
Authors: Zhu Yonghao , Hu Yanjun , Guo Qianqian , Zhao Lingqin , Xu Bin 
Published: August 2021

Abstract
Wet-treatment is usually employed to recover metals from bottom ash (BA). However, its effectiveness on regulating the leaching behavior of BA and minimizing environmental impact is still unquantified when BA is used as engineering materials. This study investigated the leaching behavior of targeted pollutants including Cu, Zn, Ni, SO42- and Cl- in fresh bottom ash (FBA) and treated bottom ash (TBA) using batch, standard column up-flow leaching and simulated rainfall down-flow leaching tests. It was firstly noted by the batch leaching that the potential ecological risk of MSWI bottom ash could not be ignore during its reutilization, and wet treatment enabled reduce the leaching concentration of Cu, Zn, Cl- and SO42- by 7.1%, 33.8%, 46.3% and 18.9%, respectively. The leaching concentrations of the targeted ions in FBA are generally higher than those of TBA. Furthermore, when BA was applied in water dynamic system such as rainfall environment, its toxicity leaching should be paid more attention onto the early leaching stage with a very high water dissolved salts release, such as Cl- and SO42-. The leaching concentration of Cu particularly exceeded the limit value before L/S=1. Meanwhile, it was also found that the leaching of SO42-, Ni, Cu and Zn in water dynamic system were directly proportionate to the liquid-to-solid (L/S) ratio. The leaching concentration of the pollutants obtained from the simulate rainfall down-flow tests was usually 4-6 times higher than those from the standard up-flow column when at the same ratio of liquid and solid.
Potential Phytoremediation of Pampa Biome Native And Invasive Grass Species Cohabiting Vineyards Contaminated With Cu In Southern Brazil
Authors: Silva Isley Cristiellem Bicalho da , Somavilla André , Soares Vanessa , Tarouco Camila , Schwalbert Raíssa , Trentin Edicarla , Quadros Fernando , Nicoloso Fernando , Ferreira Paulo , Brunetto Gustavo 
Published: August 2021

Abstract
The aims of the present study are to evaluate whether the growth of Pampa biome native grass species Axonopus affinis, Paspalum notatum and Paspalum plicatulum, as well as of invasive grass species Cynodon dactylon, is compromised by excessive Cu availability in the soil (0, 35 and 70 mg of Cu kg-1), to determine the impact of excessive Cu on the physiological responses of the investigated species and to assess whether these species have the potential to enable the phytoremediation of soils contaminated with Cu. C. dactylon presented the best performance in soil contaminated with 35 mg of Cu kg-1. In C. dactylon, the concentrations of chlorophyll b and carotenoids increased, as did the photosynthetic rate and plant growth. Phytotoxic effects of Cu in soil contaminated with 70 mg of Cu kg-1 were more severe on A. affinis and led to plant death. The other species presented reduced photosynthetic and growth rates, as well as increased activity of antioxidant enzymes such as SOD and POD. This very same Cu level has decreased photosynthetic pigment concentrations in P. notatum and P. plicatulum. On the other hand, it did not change chlorophyll a and b concentrations in C. dactylon and increased carotenoid concentrations in it. High values recorded for Cu bioaccumulation-in-grass-root factor, mainly in P. plicatulum, have indicated that the investigated plants are potential phytostabilizers. High C. dactylon biomass production - in comparison to other species - compensates for the relatively low metal concentration in its tissues by increasing metal extraction from the soil. This makes C. dactylon more efficient in the phytoremediation process than other species.
The Value of West Nile Virus RNA Detection by Real-time PCR in Urine Samples From Patients With Neuroinvasive Forms
Authors: gdoura Mariem , Fares Wasfi , Bougatef Souha , Inoubli Amine , Touzi Henda , Hogga Nahed , Dhifallah Imen Ben , Hannachi Naila , Argoubi Aida , Kacem Saoussen , Karray Hela , Alaya Nissaf Ben , Triki Henda 
Published: August 2021

Abstract
Introduction Routine laboratory screening is based on the detection of WNV specific IgM and IgG in blood and cerebrospinal fluid. Confirmation is then classically applied RT-PCR in CSF, which often give negative results due to too short virorachia and late sampling.RT-PCR was applied-for the first time for routine diagnosis purpose-on urine samples.Methods During 2018 outbreak in Tunisia, 107 patients presented WNV neurologic symptoms and were positive for WNV serology. Of them, 95 patients were sampled for urine and 35 were sampled for CSF. Qualitative RT-PCR was performed on both type of samples. Results WNV RNA was detected in 50,5% of urine samples (48/95) and in 2,8% of CSF samples (1/35). WNV RNA was detectable from 1 to 41 day from symptom onset, however, positive urine rate was 53,1% during the first 10 days from symptom onset. The proportions of urine-positive and urine-negative samples, based on day of collection, showed no statistical difference ( p >0,005). Cycle threshold values ranged from 12 to 39, with no correlation with the day of collection. The lowest Ct value was detected for urine sampled on day 5 after symptom onset. A statistically significant difference was found between age groups of confirmed and non confirmed cases ( p <0,001). Discussion/Conclusion Our study reports the use of RT-PCR on urine samples as a confirmatory diagnostic tool for WNV probable cases during an outbreak. Our findings underline the reliability, and rapidity of this confirmatory tool, even late and show its superiority on CSF investigation.
Inhibition of Neuropilin-1 improves non-alcoholic fatty liver disease via PI3K/AKT/mTOR signaling in high-fat-diet induced obese mouse
Authors: Zhou Jian , Xu Sihuan , Zhu Yue , Li Xin , Wang Ao , Liu Kun , Hu Junfang , Li Li , Liu Yan 
Published: August 2021

Abstract
Background

Research findings indicate Neuropilin-1 plays a critical role in lipid metabolism and obesity-associated insulin resistance, on such a basis, this study aims to explore the effects and working mechanism of Neuropilin-1 inhibition on the non-alcoholic fatty liver disease in high-fat-diet induced obese mice.
Methods

Firstly, the pcDNA3.1-NRP-1 recombinant plasmid containing Neuropilin-1 gene and the Neuropilin-1 gene RNA interference plasmid shRNA-NRP1 were successfully constructed. A total of 36 C57BL/6 mice were randomly assigned to 6 groups, blank group, control group, pcDNA3.1 injection group, pcDNA3.1-NRP-1 injection group, pGenesil-1.1 injection group and shRNA-NRP1 injection group. Expression of phospho-PI3K, phospho-AKT, phospho-mTOR and Neuropilin-1 in liver was measured as well as body and liver weight, blood glucose, serum transaminases and lipid levels of the mice.
Results

The weight and liver mass of high-fat-diet fed mice injected with pcDNA3.1-NRP-1 were significantly higher than those from the control group, but their body weight and liver mass decreased significantly after shRNA-NRP1 injection. The results also showed that Neuropilin-1 expression can significantly influence the severity of hepatic steatosis in high-fat-diet fed mice, decreased serum FPG, LDL, AST, ALT levels and the expression of TNF-α, IL-1β, and IL-6 mRNA. In addition, the Neuropilin-1 expression will also influence the p-PI3K, p-AKT and p-mTOR in mice.
Conclusions

This study concluded that the inhibition of Neuropilin-1 could improve Non-alcoholic fatty liver disease by decreasing body weight and reduce inflammation in high-fat-diet induced obese mice by modulating the PI3K/AKT/mTOR signaling pathway.