bluuebunny/crossref_metadata_2025_split huggingface dataset:
PELATIHAN JIWA KEPEMIMPINAN KEPADA KOMUNITAS PENCINTA ALAM KELURAHAN PONDOK PUCUNG
Authors: Oktavianti Nopi , Nariah Nariah , Imbron Imbron , Safiih Abdul Rahman , Nuraldy Hafis Laksmana 
Published: June 2021

Kegiatan Pengabdian ini bertujuan untuk memberikan bekal kepemimpinan dan manajemen organisasi kepada seluruh anggota organisasi dalam bentuk penyuluhan mental kepemimpinan, pembinaan kepemimpinan, kekompakan, tanggung jawab dalam menjalankan kegiatan organisasi melatih dan mengembangkan jiwa kepemimpinan kepada seluruh anggota Komunitas Pecinta Alam ARIMBIN yang berada di Kelurahan Pondok Pucung, kelak siapa saja yang nantinya menjadi pemimpin organisasi tersebut sudah mempunyai bekal karakter pemimpin yang layak agar mampu memegang peran penting didalam organisasi dengan baik. Seorang pemimpin harus mempunyai karakter yang tegas, disegani, wibawa, bisa mengajak anggota lain untuk bekerja dalam tim, bisa memotivasi anggota, bisa mengambil keputusan dengan pertimbangan yang matang dan lain sebagainya. Hasil kegiatan ini menunjukan bahwa sebelum pelaksanaan PKM ini seluruh anggota masih kurang menguasai materi tentang kepemimpinan, namun setelah pelaksanaan kegiatan dilakukan peserta kegiatan PKM ini sudah dapat memahami aspek-aspek kepemimpinan yang sebenarnya sangat luas, ini semua dapat terlihat dari hasil sesi tanya jawab yang diberikan kepada peserta saat kegiatan PKM ini berlangsung. Metode yang digunakan adalah memberikan pelatihan melalui pembekalan materi kepemimpinan kepada seluruh anggota Komunitas Pecinta Alam ARIMBIN kemudian berdiskusi bersama terkait permasalahan yang ada didalam organisasi tersebut sehingga dapat memberikan solusi yang tepat.
Pengaruh Penambahan Kacang Hijau (Vigna Radiata) Terhadap Karakteristik Organoleptik, Nilai Gizi dan Antioksidan Sari Biji Nangka (Artocarpus Heterophyllus) Sebagai Minuman Fungsional
Authors: Nurlin Nurlin , Tamrin Tamrin , Hermanto Hermanto 
Published: June 2021

Penelitian ini bertujuan untuk mempelajari pengaruh penambahan sari kacang hijau terhadap organoleptik, nilai gizi dan aktivitas antioksidan minuman sari biji nangka. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), yaitu terdiri dari 5 perlakuan perlakuan KO (biji nangka 100% : sari kacang hijau 0%), K1 (biji nangka 90% : sari kacang hijau 10%), K2 (biji nangka 80% : sari kacang hijau20%), K3 (biji nangka 70% : 30%), K4 (biji nangka 60% : sari kacang hijau 40%). Data dianalisis menggunakan Analysis of Variances (ANOVA). Hasil penelitian menunjukkan bahwa perlakuan penambahan sari kacang hijau berpengaruh sangat nyata terhadap organoleptik aroma, warna, dan rasa berpengaruh nyata terhadap organoleptik rasa pada perlakuan K0 dan K4 diperoleh nilai untuk aroma 3,56 (suka), rasa 4,39(suka), dan warna 4,61 (sangat suka). Serta analisis kandungan gizi meliputi Kadar protein 2,53 %, glukosa 19,82 %, viskositas 6,60 cp dan aktivitas antioksidan IC50219,45. Berdasarkan SNI bahwa produk minuman sari biji nangka dengan penambahan sari kacang hijau belum memenuhi standar untuk kadar glukosa maksimum 5%.
DAYA TERIMA CUP CAKE SUBSTITUSI IKAN BANDENG DAN UBI JALAR KUNING
Authors: Hartati Yuli , Novelia Anita , Terati Terati , Purnamasari Fajriah 
Published: June 2021

Cup cake merupakan salah satu makanan selingan yang disukai oleh anak-anak sampai dewasa. Sebagai makanan yang cukup digemari, maka cup cake dapat dijadikan sebagai makanan tambahan untuk anak gizi kurang. Penelitian ini bertujuan untuk mengetahui daya terima cup cake dengan dengan substitusi ikan bandeng dan ubi jalar kuning.. Penelitian ini menggunakan rancangan acak lengkap non faktorial. Formula yang dibuat ada 3 dengan komposisi ikan bandeng dan ubi jalar kuning Formula 1 (F1) : 50 % : 50 %; Formula 2 (F2) : 60 % : 40 %; Formula 3 (F3) : 70 % : 30 %. Panelis yang digunakan untuk menilai daya terima adalah panelis setengah terlatih. Hasil penelitian menunjukkan bahwa formula yag paling disukai oleh panelis adalah formula 3 yang memiliki skor tertinggi pada rasa, aroma, warna dan tekstur. Komposisi ikan bandeng dan ubi jalar kuning sebanyak 70 % : 30 % mempengaruhi rasa, aroma, warna dan tekstur cup cake.
EVALUATION OF LYMPHATIC FILARIASIS AFTER TWO ROUNDS OF MASS DRUG ADMINISTRATION IN LAU LOCAL GOVERNMENT AREA OF TARABA STATE NIGERIA
Authors: Akafyi D. E. , Ndams I. S. , Luka S. A. , Ojeleye F. S. , Elkanah S. O. , Kamba B. 
Published: June 2021

This study was undertaken to evaluate the effects of Mass Drug Administration (MDA) on Wuchereria bancrofti (microfilariae) after two rounds of combined Ivermectin and Albendazole distribution. A total of 221 participants were recruited in three communities in Lau Local Government Area of Taraba State by convenience sampling method. Questionnaires and physical examinations were used to assess clinical manifestations associated with the infection. Blood samples were collected by finger prick method and stained with Giemsa stain for examination to establish the presence of W. bancrofti while immunochromatographic card test was performed to determine the presence of filarial antigen in serum. Previous data were used to determine the pre-drug prevalence of the parasite. The results showed that the drug did not significantly reduce the clinical manifestations reported among the patients. The microfilariae prevalence and microfilaria mean density after two rounds of drug administration was 19.5% and 1.49%, while the pre- MDA prevalence and microfilaria mean density was 27.8% and 2.44% respectively. There was a statistically significant decrease of microfilaria prevalence (P<0.05) after two rounds of MDA. There was no significant effect of MDA by age, sex and occupation-related microfilariae prevalence in the study area. In conclusion, the study reveals that microfilaria prevalence and load decreased after two rounds of MDA of combined Ivermectin and Albendazole distribution amongst the studied populations. Routine evaluation of the MDA is required to assess the impact of the drug for the eventual elimination of the infection.
Integrated Transcriptome Profiling Revealed That Elevated Long Non-Coding RNA-AC007278.2 Expression Repressed CCR7 Transcription in Systemic Lupus Erythematosus
Authors: You Yi , Zhao Xingwang , Wu Yaguang , Mao Jiangming , Ge Lan , Guo Junkai , Zhao Chenglei , Chen Dong , Song Zhiqiang 
Published: June 2021

PurposeSystemic lupus erythematosus (SLE) is a serious autoimmune disease. Its molecular pathogenesis, especially the long non-coding RNA (lncRNA) function, remains unclear. We want to investigate the lncRNA dysregulation profile and their molecular mechanisms in SLE.MethodsIn this study, we analyzed the transcriptome profiles (RNA-seq) of peripheral blood mononuclear cells (PBMCs) from SLE patients and two published transcriptome datasets to explore lncRNA profiles. The differentially expressed lncRNAs were confirmed by quantitative real-time PCR in another set of female patients. We constructed the lncRNA-mRNA regulatory networks by performing weighted gene co-expression network analysis (WGCNA). Dysregulated lncRNA AC007278.2 was repressed by short hairpin RNA (shRNA) in Jurkat cells. Dual-luciferase reporter gene assay was performed to investigate the regulatory mechanism of AC007278.2 on target gene CCR7.ResultsWe observed dominant up-regulation of transcripts, including mRNAs and lncRNAs, in SLE patients. By WGCNA method, we identified three modules that were highly related to SLE. We then focused on one lncRNA, AC007278.2, with a T-helper 1 lineage-specific expression pattern. We observed consistently higher AC007278.2 expression in SLE patients. Co-expression network revealed that AC007278.2 participated in the innate immune response and inflammatory bowel disease pathways. By knocking down AC007278.2 expression, we found that AC007278.2 could regulate the expression of inflammatory and cytokine stimulus response-related genes, including CCR7, AZU1, and TNIP3. AC007278.2 inhibits the functional CCR7 promoter to repress its transcription, thereby regulating autoimmunity and follicular T-helper cell differentiation.ConclusionIn summary, our study indicated the important regulatory role of lncRNAs in SLE. AC007278.2 may be treated as a novel biomarker for SLE diagnosis and treatment.
High Level of Soluble CD146 In Cerebrospinal Fluid Might be a Biomarker of Severity of Anti-N-Methyl-D-Aspartate Receptor Encephalitis
Authors: Li Qing , Chen Jinglong , Yin Mengzhuo , Zhao Jun , Lu Fuchang , Wang Zhanhang , Yu Xiaoqi , Wang Shuangyan , Zheng Dong , Wang Honghao 
Published: June 2021

BackgroundDisruption of the blood–brain barrier (BBB) is an important pathophysiological process of anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis. A recent multi-center study showed that soluble (s) CD146 is a potential biomarker for monitoring early BBB damage and central nervous system inflammation, but little is known about sCD146 in anti-NMDAR encephalitis.MethodTwenty-three anti-NMDAR encephalitis patients and seventeen controls with non-inflammatory neurological diseases were recruited. sCD146 and inflammatory cytokines in cerebrospinal fluid (CSF) and serum were detected by ELISA. Modified Rankin scale (mRS) scores were used to assess the neurological status of each patient. A follow-up review was completed three months after discharge.ResultssCD146 levels in the CSF of patients with the acute stage anti-NMDAR encephalitis were significantly increased compared with controls and accompanied by increases in TNF-α, IL-6 and IL-10. CSF sCD146 was positively correlated with neuroinflammatory factors in the CSF and with mRS score. Three months after effective treatment, CSF sCD146 in patients was significantly decreased but remained significantly different compared with the controls.ConclusionOur data suggested that higher expression of CSF sCD146 correlated with more serious neurological damage. Therefore, levels of CSF sCD146 may represent a promising indicator for monitoring disease and optimizing clinical treatment decisions in the early stages of anti-NMDAR encephalitis.
Non-Typhoidal Salmonella and the Risk of Kawasaki Disease: A Nationwide Population-Based Cohort Study
Authors: Chen Thomas Yen-Ting , Chou Mei-Chia , Lai Jung-Nien , Chiu Lu-Ting , Chang Renin , Hung Yao-Min , Wei James Cheng-Chung 
Published: June 2021

ObjectiveThe aim of this study was to investigate the relationship between non-typhoidalSalmonella(NTS) infection and the risk of Kawasaki disease (KD) by using a nationwide population-based data set in Taiwan.MethodsIn this retrospective cohort study, we enrolled 69,116 patients under 18 years of age, with NTS from January 1st, 2000, to December 31st, 2013, using the population-based National Health Insurance Research Database of Taiwan. A comparison group without NTS was matched (at a 1:4 ratio) by propensity score. The two cohorts were followed from the initial diagnosis of NTS until the date of KD development or December 31st, 2013. Cox proportional hazard regression analysis was conducted to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) after adjusting for covariates. Also, we conducted sensitivity analyses to examine our findings.ResultsAfter adjusting for covariates, the risk of KD for the children with NTS was significantly higher than that of the comparison group (hazard ratio = 1.31; 95% confidence interval = 1.03-1.66; p &lt; 0.01). Stratified analysis showed that the associated risk of the investigated outcome was significant in children aged ≤2 years (aHR= 1.31, 95% C.I. 1.02-1.69), in female patients (aHR= 1.46, 95% C.I. 1.03-2.08), and in those without allergic diseases.ConclusionsNTS is associated with an increased risk of KD in Taiwanese children.
Epidemiology Profile of Viral Meningitis Infections Among Patients in Qatar (2015–2018)
Authors: Mathew Shilu , Al Khatib Hebah A. , Al Ansari Khalid , Nader Joanne , Nasrallah Gheyath K. , Younes Nadin N. , Coyle Peter V. , Al Thani Asmaa A. , Al Maslamani Muna A. , Yassine Hadi M. 
Published: June 2021

Background:Little is known about the etiology of meningitis in the MENA region, including Qatar. Viral agents are considered the major cause for meningitis worldwide. Here, we present primary data about the etiology and clinical and demographic characteristics of viral meningitis (VM) in Qatar between 2015 and 2018.Methods:We retrospectively collected data from Hamad Medical Corporation (HMC), which provides about 80% of healthcare services in Qatar. Data were collected for the period between 2015 and 2018. During this time period, 6,705 specimens were collected from patients with suspected meningitis attending HMC and primary healthcare centers. These specimens were tested for a panel of viruses using the “FTD Viral meningitis” multiplex real-time PCR kit that detects Adenovirus (ADV), Human herpesvirus 1&amp;2 (HSV1 and HSV2), Epstein–Barr virus (EBV), Enteroviruses (EV), Cytomegalovirus (CMV), Varicella zoster virus (VZV), and Parechovirus (PV).Results:Only 10.9% (732/6,705) of all suspected meningitis cases were caused by viral agents. 60.9% of the reported cases were males, compared to 39.1% in females. Most of the infections (73.9%) were reported in children younger than 10 years of age. EV were identified as the main causative agent (68.7%), followed by EBV (7.5%) and ADV (6.8%). Other viral agents including VZV, PV, HSV-1, and HSV-2 were also detected with a lower frequency. Confirmed VM were more prevalent among Qatari subjects compared to other nationalities. We observed no specific seasonality of viral agents, but a slight rise was recorded during the spring seasons (March to June). Fever (59.4%, 435/732) and acute central nervous system (CNS) infection (15.6%, 114/732) were initial symptoms of most cases.Conclusion:This is the first report about the molecular epidemiology of VM in Qatar. In line with the international records, our data showed that EV is responsible for 68.7% of Qatar's VM cases. Further studies are needed to genotype and serotype the identified viruses.
Late Gastrointestinal Tolerance After Prostate Radiotherapy: Is the Anal Canal the Culprit? A Narrative Critical Review
Authors: Sargos Paul , Faye Mame Daro , Bacci Manon , Supiot Stéphane , Latorzeff Igor , Azria David , Niazi Tamim M. , Vuong Te , Vendrely Véronique , de Crevoisier Renaud 
Published: June 2021

IntroductionLate gastro-intestinal toxicities (LGIT) secondary to pelvic radiotherapy (RT) are well described in the literature. LGIT are mainly related to rectal or ano-rectal irradiation; however, involvement of the anal canal (AC) in the occurrence of LGIT remains poorly described and understood.Materials and MethodsThe aim of this work was to explore the potential role of the AC in the development of LGIT after prostate irradiation and identify predictive factors that could be optimized in order to limit these toxicities. This narrative literature review was realized using the Pubmed database. We identified original articles published between June 1997 and July 2019, relating to LGIT after RT for localized prostate cancer and for which AC was identified independently. Articles defining the AC as part of an anorectal or rectal volume only were excluded.ResultsA history of abdominal surgery or cardio-vascular risk, anticoagulant or tobacco use, and the occurrence of acute GIT during RT increases the risk of LGIT. A dose-effect relationship was identified between dose to the AC and development of LGIT. Identification and contouring of the AC and adjacent anatomical structures (muscles or nerves) are justified to apply specific dose constraints. As a limitation, our review mainly considered on 3DCRT which is no longer the standard of care nowadays; we did not identify any reports in the literature using moderately hypofractionated RT for the prostate and AC specific dosimetry.ConclusionThese results suggest that the AC may have an important role in the development of LGIT after pelvic RT for prostate cancer. The individualization of the AC during planning should be recommended in prospective studies.
Establishment of a Prognostic Nomogram for Patients With Locoregionally Advanced Nasopharyngeal Carcinoma Incorporating TNM Stage, Post-Induction Chemotherapy Tumor Volume and Epstein-Barr Virus DNA Load
Authors: Jiang Yu-Ting , Chen Kai-Hua , Yang Jie , Liang Zhong-Guo , Qu Song , Li Ling , Zhu Xiao-Dong 
Published: June 2021

ObjectivesTo establish and validate an effective nomogram to predict clinical outcomes for patients with locoregionally advanced nasopharyngeal carcinoma (LA-NPC).Materials and MethodsThe clinicopathological parameters and follow-up information of 402 locoregionally advanced NPC patients (training cohort, n = 302; validation cohort, n = 100) were retrospectively enrolled. The nomogram was built with the important prognostic variables identified by Cox regression analysis. Overall survival (OS) and progression-free survival (PFS) were the primary and secondary endpoints, respectively. The predictive power and clinical utility of the nomogram were assessed using the Harrell concordance index (C-index), calibration curve, and decision curve analysis. We compared the eighth staging system model with the nomogram to analyze whether the model could improve the accuracy of prognosisResultsEpstein–Barr virus (EBV) DNA load, the gross tumor volume (GTVnx), and cervical lymph node tumor volume (GTVnd) after induction chemotherapy were the independent predictors of OS and PFS. The calibration curves indicated superb agreement between the nomogram-predicted probabilities and observed actual probabilities of survival. The C-index and area under the receiver operator characteristic curve (AUC) of the nomogram integrating these significant factors and N stage, and TNM stage were higher than those of the eighth TNM system alone. In addition, the decision curve analyses demonstrated the clinical value and higher overall net benefit of the nomogram. High-risk groups identified by the nomogram had significantly poorer OS and PFS than the low-risk group (p &lt; 0.05).ConclusionsThe multidimensional nomogram incorporating TNM stage, EBV DNA load, and tumor volume after induction chemotherapy led to a more precise prognostic prediction and could be helpful for stratifying risk and guiding treatment decisions in locoregionally advanced NPC patients who have undergone induction chemotherapy and concurrent chemoradiation.
Racial Differences in Clinical Outcomes for Metastatic Renal Cell Carcinoma Patients Treated With Immune-Checkpoint Blockade
Authors: Olsen T. Anders , Martini Dylan J. , Goyal Subir , Liu Yuan , Evans Sean T. , Magod Benjamin , Brown Jacqueline T. , Yantorni Lauren , Russler Greta Anne , Caulfield Sarah , Goldman Jamie M. , Harris Wayne B. , Kucuk Omer , Carthon Bradley C. , Master Viraj A. , Nazha Bassel , Bilen Mehmet Asim 
Published: June 2021

BackgroundImmune-checkpoint-inhibitors (ICIs) have become the cornerstone of metastatic renal-cell-carcinoma (mRCC) therapy. However, data are limited regarding clinical outcomes by race. In this study, we compared the real-world outcomes between African American (AA) and Caucasian mRCC patients treated with ICIs.MethodsWe performed a retrospective study of 198 patients with mRCC who received ICI at the Emory Winship Cancer Institute from 2015-2020. Clinical outcomes were measured by overall survival (OS), progression-free survival (PFS), and overall response rate (ORR) defined as a complete or partial response maintained for at least 6 months per response evaluation criteria in solid tumors version 1.1. Univariate and multivariable analyses were carried out for OS and PFS by Cox proportional-hazard model and ORR by logistical-regression model. Descriptive statistics compared rates of immune-related adverse events (irAEs) and non-clear-cell-RCC (nccRCC) histology were assessed using Chi-square test.ResultsOur cohort was comprised of 38 AA and 160 Caucasian patients. Most were diagnosed with clear-cell-RCC (ccRCC) (78%) and more than half received (57%) PD-1/PD-L1 monotherapy. Most patients were intermediate or poor-risk groups (83%). Comparing to Caucasians, our AA cohort contained more females and nccRCC cases. Kaplan-Meier method showed AAs had no statistically different median OS (17 vs 25 months, p=0.368) and PFS (3.1 vs 4.4 months, p=0.068) relative to Caucasian patients. On multivariable analysis, AA patients had significantly shorter PFS (HR=1.52, 95% CI: 1.01-2.3, p=0.045), similar ORR (OR=1.04, 95% CI: 0.42-2.57, p=0.936) and comparable OS (HR=1.09, 95% CI: 0.61-1.95, p=0.778) relative to Caucasians.ConclusionsOur real-world analysis of ICI-treated mRCC patients showed that AAs experienced shorter PFS but similar OS relative to Caucasians. This similarity in survival outcomes is reassuring for the use of ICI amongst real-world patient populations, however, the difference in treatment response is poorly represented in early outcomes data from clinical trials. Thus, the literature requires larger prospective studies to validate these findings.
Lactoferrin Against SARS-CoV-2: In Vitro and In Silico Evidences
Authors: Campione Elena , Lanna Caterina , Cosio Terenzio , Rosa Luigi , Conte Maria Pia , Iacovelli Federico , Romeo Alice , Falconi Mattia , Del Vecchio Claudia , Franchin Elisa , Lia Maria Stella , Minieri Marilena , Chiaramonte Carlo , Ciotti Marco , Nuccetelli Marzia , Terrinoni Alessandro , Iannuzzi Ilaria , Coppeda Luca , Magrini Andrea , Bernardini Sergio , Sabatini Stefano , Rosapepe Felice , Bartoletti Pier Luigi , Moricca Nicola , Di Lorenzo Andrea , Andreoni Massimo , Sarmati Loredana , Miani Alessandro , Piscitelli Prisco , Valenti Piera , Bianchi Luca 
Published: June 2021

Lactoferrin (Lf) is a cationic glycoprotein synthetized by exocrine glands and is present in all human secretions. It is also secreted by neutrophils in infection and inflammation sites. This glycoprotein possesses antimicrobial activity due to its capability to chelate two ferric ions per molecule, as well as to interact with bacterial and viral anionic surface components. The cationic features of Lf bind to cells, protecting the host from bacterial and viral injuries. Its anti-inflammatory activity is mediated by the ability to enter inside the nucleus of host cells, thus inhibiting the synthesis of proinflammatory cytokine genes. In particular, Lf down-regulates the synthesis of IL-6, which is involved in iron homeostasis disorders and leads to intracellular iron overload, favoring viral replication and infection. The well-known antiviral activity of Lf has been demonstrated against DNA, RNA, and enveloped and naked viruses and, therefore, Lf could be efficient in counteracting also SARS-CoV-2 infection. For this purpose, we performed in vitro assays, proving that Lf exerts an antiviral activity against SARS-COV-2 through direct attachment to both SARS-CoV-2 and cell surface components. This activity varied according to concentration (100/500 μg/ml), multiplicity of infection (0.1/0.01), and cell type (Vero E6/Caco-2 cells). Interestingly, the in silico results strongly supported the hypothesis of a direct recognition between Lf and the spike S glycoprotein, which can thus hinder viral entry into the cells. These in vitro observations led us to speculate a potential supplementary role of Lf in the management of COVID-19 patients.