bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)
Authors: Kashinskaya E.N. , Simonov E.P. , Vlasenko P.G. , Markevich G.N. , Shokurova A.V. , Andree K.B. , Solovyev M.M. 
Published: December 2021

Abstract
Using the approach of sequencing the V3–V4 region of the 16S rRNA gene, we have analyzed the bacterial diversity associated with the gut and “body” (other parts of nematode after dissection: cuticle, epidermis and longitudinal muscles, etc) of Cystidicola farionis parasitizing the swim bladder of different morphotypes of the nosed charr. Comparisons of the gut microbiota of nematodes with their “body” has revealed that the associated microbiota are closely related to each other. Taxonomic analysis indicated that the relative abundances of the dominant nematode-associated bacteria varied with individual fish. The common dominant microbiota of the gut and “body” of nematodes were represented by Aeromonas, Pseudomonas, Shewanella, and Yersinia, while the associated microbiota of the swim bladder of the nosed charr was dominated by Acinetobacter, Cetobacterium, Pajaroellobacter, Paracoccus, Pseudomonas, Shewanella. By comparing the associated microbiota of nematode parasitizing the different morphotypes of the nosed charr the difference in richness estimates (number of OTU’s and Chao1) were revealed between the N1g and N2 morphs.
Perencanaan Transformasi Ternak Sapi Perah ke Wisata Edukasi Susu Segar di Jatirejo Semarang
Authors: Sitinjak Elizabeth Lucky Maretha , Hartajanie Laksmi , Lindayani  , Setiawan Florentinus Budi , Matitaputty Shandy Jannifer 
Published: December 2021

Tujuan Pengabdian Kepada Masyarakat Kelurahan Jatirejo, Kecamatan Gunungpati Kota Semarang adalah membantu Kelompok Ternak Sapi Perah (KTSP) khususnya, untuk dapat bertransformasi dari konsep peternakan sederhana ke area wisata edukasi ternak sapi perah. Pada masa awal pandemi Covid-19 di tahun 2020, populasi sapi perah naik sebesar 0,53% secara nasional, namun populasi sapi perah di Jawa tengah menurun sebesar 0,65% dan produksinya pun menurun sebesar 2,94%. Tahun 2021, permintaan semakin banyak untuk Susu Sapi Perah, oleh karena olahan susu perah menjadi susu segar yang memiliki kandungan gizi sangat lengkap, kaya protein, lemak, karbohidrat, mineral, dan vitamin. Kandungan nutrisi susu yaitu kalsium, fosfor, zinc, vitamin A, D, B12, B2, asam amino dan asam pantotenat. Manfaat susu sapi perah menjadi susu sapi segar dengan beberapa pengolahan yang sesuai dengan kebutuhan masyarakat sekitar Jatirejo dan konsumen. Ada beberapa teknologi pengolahan sederhana yang dapat diterapkan di lingkungan peternak untuk mengawetkan susu, di antaranya pasteurisasi dan fermentasi (yoghurt, yakult, dan kefir). Metode pengolahan tersebut membuat umur simpan dapat diperpanjang dan dihasilkan susu yang memiliki flavor dan tekstur yang berbeda sehingga dapat meningkatkan nilai jual produk tersebut. Selain itu, tempat pemerahan susu sapi dan toko tempat penjualan susu segar serta olahannya didesain instagramable. Transformasi dari susu sapi perah menjadi susu segar dengan aneka pengolahan, disertai konsep pemasaran wisata edukasi ternak sapi. Studi kelayakan finansial mendukung usaha transformasi ternak sapi perah, dengan konsep tempat pemerahan sapi yang higienis, ramah lingkungan, dan menarik para milenial untuk minum susu segar.
ConvChrome: Predicting Gene Expression Based on Histone Modifications
Using Deep Learning Techniques
Authors: Hamdy Rania , Maghraby Fahima A. , Omar Yasser M.K. 
Published: December 2021

Background:
Gene regulation is a complex and dynamic process that not only depends on
the DNA sequence of genes but is also influenced by a key factor called epigenetic mechanisms. This
factor, along with other factors, contributes to changing the behavior of DNA. While these factors cannot
affect the structure of DNA, they can control the behavior of DNA by turning genes "on" or "off,"
which determines which proteins are transcribed.

Objective:
This paper will focus on the histone modification mechanism; histones are the group of proteins
that bundle the DNA into a structural form called nucleosomes (coils); The way these histone proteins
wrap DNA determines whether or not a gene can be accessed for expression. When histones are
tightly bound to DNA, the gene is unable to be expressed, and vice versa. It is important to know histone
modifications’ combinatorial patterns and how these combinatorial patterns can affect and work
together to control the process of gene expression.

Methods:
In this paper, ConvChrome deep learning methodologies are proposed for predicting the gene
expression behavior from histone modifications data as an input to use more than one convolutional
network model; this happens in order to recognize patterns of histones signals and interpret their spatial
relationship on chromatin structure to give insights into regulatory signatures of histone modifications.

Results and Conclusion:
The results show that ConvChrome achieved an Area under the Curve (AUC)
score of 88.741%, which is an outstanding improvement over the baseline for gene expression classification
prediction task from combinatorial interactions among five histone modifications on 56 different
cell types.
Vitamin C Inhibits Angiotensin-Converting Enzyme-2 in Isolated Rat Aortic
Ring
Authors: Amssayef Ayoub , Bouadid Ismail , Eddouks Mohamed 
Published: December 2021

Aims:
The study aimed to assess the inhibitory effect of Vitamin C on angiotensin-converting
enzyme 2.

Background:
Coronavirus disease 2019 (COVID-19) is a disease caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2), which uses angiotensin-converting enzyme 2 (ACE-II)
as the first route to infect human cells. Accordingly, agents with potential inhibition of ACE-II receptors
might be effective in the prevention and management of COVID-19.

Objective:
The goal of this work was to assess the possible inhibitory effect of ACE-II on ascorbic
acid using an ex vivo approach based on the inhibition of diminazene-induced vasorelaxation.

Material and Methods:
In the present study, diminazene was used as a known specific inhibitor
of ACE-II. Then, the vasorelaxant effect of ascorbic acid on diminazene-induced relaxation was examined
using isolated aortic rings. All experiments of this study were evaluated on isolated aortic
rings precontracted by epinephrine.

Results:
The results confirmed that diminazene-induced vasorelaxation in a dose-dependent manner.
More interestingly, ascorbic acid inhibited diminazene-induced vasorelaxation in a dose-dependent
manner.

Conclusion:
This investigation provides valuable experimental proof of the efficacy of ascorbic
acid (Vitamin C) on inhibiting ex vivo vascular angiotensin-converting enzyme II, which is known
among the pharmacological targets of anti-COVID-19 drugs.
A Scoping Review of Men's Health Situation in Primary Health Care
Authors: Leal Borges Cléa Conceição , de Sousa Anderson Reis , Araújo Isabella Félix Meira , Ortiz Rodríguez José Edward , Escobar Oscar Javier Vergara , Martins Ridalva Dias , Pereira Álvaro , Fernandes Fernando Lannes 
Published: December 2021

Introduction:
There has been an increasing number of studies that have evidenced the profile of male morbidity and mortality, their causes and related consequences, and their association with the low demand and men's permanence in the services offered in Primary Health Care (PHC).

Objective:

The objective of the study was to map the literature on men's health situation in Primary Health Care.

Methods:
A scoping review, according to the Joanna Briggs Institute protocol, with the PCC acronym (P – men, C – health situation, C – in primary care), was conducted in the Medline/Pubmed, COCHRANE, Web of Science, SCOPUS, Science Direct, LILACS, BDENF, MEDCARIBE, and SciELO databases, using the descriptors “Men”, “Men's Health”, “Health Services Needs and Demand”, “Primary Health Care” in three information sources (EBSCO, PubMed and SciELO).

Results:
Men's health situation in Primary Health Care is defined by the influence of the social construction of the masculinities and cultural aspects in the adoption of health care behaviors and practices, in the way in which men present their health complaints, demands, and needs, and establish bonds with the health professionals and the services.

Conclusion:
There is a number of sociocultural factors inherent to masculinities, institutional factors of the services' organization, and relational factors of men with the health professional that intervene together with the health situation.
Determining Priorities in the Aboriginal and Islander Mental Health Initiative for Youth App Second Phase Participatory Design Project: Qualitative Study and Narrative Literature Review
Authors: Povey Josie , Sweet Michelle , Nagel Tricia , Lowell Anne , Shand Fiona , Vigona Jahdai , Dingwall Kylie M 
Published: December 2021

Background
Digital mental health tools can promote access to culturally safe early intervention mental health services for Aboriginal and Torres Strait Islander young people. Participatory design methodology facilitates user engagement in the co-design of digital resources. However, several challenges have been identified that limit the methodological rigor of this approach.

Objective
This paper aims to present an in-depth account of the second phase of participatory design in the development of the Aboriginal and Islander Mental Health Initiative for Youth (AIMhi-Y) app.

Methods
A first idea storyboard, generated from a formative phase of the AIMhi-Y project, was refined through a series of youth co-design workshops and meetings. A narrative review of the literature, 6 service provider interviews, and engagement with an expert reference group also informed the design process. Generative design activities, storyboarding, discussions, and voting strategies were used.

Results
The participatory design process identified the app features preferred by young people and service providers and assessed their alignment with current recommendations from the scientific literature. Findings from the co-design process are presented across 9 app characteristic domains. Integration of findings into app design proved complex. Although most preferred features identified by young people were included to some degree, other inclusions were restricted by budget, time, and the need to integrate best practice recommendations. A process of prioritization was required.

Conclusions
Participatory design is often cited in the development of digital mental health resources; however, methods are diverse and often lack detailed descriptions. This study reports the outcomes and strategies used to determine priorities in the second phase of the development of the AIMhi-Y app. We provide an example and the key learnings to inform others seeking to use participatory design with a similar cohort.
Collaborative Annotation as a Teaching Tool
Authors: Bauer Matthias , Lahrsow Miriam 
Published: December 2021

The guidelines presented here were developed in a seminar aimed at M.A. and advanced B.A. students. They are based on narratological theories by Marie-Laure Ryan, Gérard Genette, William Nelles, Ansgar Nünning, John Pier, and Viveca Füredy. Our contribution focuses on how collaborative annotation tasks can be used in university seminars, especially in the context of teaching students how to critically assess and compare theoretical frameworks and definitions. We also highlight the students’ impression that developing and using annotating guidelines improved their close-reading skills and that the task sensitised them to some of the core challenges of distant reading (e.g. questions of ambiguity and interpretation).
El yo situado y relacional en Nishida y Watsuji: una aproximación desde el paradigma de la intimidad de Kasulis
Authors: Bouso Raquel 
Published: December 2021

En su obra Intimacy or Integrity: Philosophy and Cultural Difference (originalmente 1988 Gilbert Ryle Lectures, publicada en 2002), Thomas P. Kasulis identifica dos tipos de orientaciones “intimidad” e “integridad” que condicionan tanto las relaciones interpersonales como nuestra relación con el mundo. Si bien ambas orientaciones se pueden hallar tanto en las tradiciones filosóficas occidentales como en las asiáticas, puede decirse que la primera predomina en buena parte de la filosofía japonesa. A partir del paradigma de la intimidad descrito por Kasulis, examinaremos dos creaciones conceptuales de Nishida Kitarō (1870-1945) y Watsuji Tetsurō (1889-1960) respectivamente, el “yo como lugar” y el “ambiente geocultural”. De este modo, mostraremos cómo emerge un modo de comprender el “yo” como algo situado y relacional que nos permite pensar un modo de ser ecológico-comunitario.
Accounting Firm Association Membership and Small Audit Firm Growth
Authors: Ahn Jaehan , Akamah Herita , Bills Kenneth L. , Saunders K. Kelli 
Published: December 2021

SUMMARY
In this study, we explore a topic of primary concern to small audit firms—attracting public audit clients. A potential avenue available to small audit firms to enhance their visibility and legitimacy among potential public audit clients is to join an association of accounting firms (ACAP 2008; GAO 2008). We examine whether small audit firms with accounting association membership have greater public clientele growth than their peer audit firms without association membership. We find that member firms experience public clientele growth as measured by number of clients and revenues audited. We find that this growth is impacted by both gaining new entrants to the audit market and winning over clients from competitors. Further, we find that the reputation of associations positively affects the clientele growth seen by member firms. In additional analyses, we also find some evidence of private client growth of association members using LexisNexis® Company Dossier data.
Is There a Dark Side to Societal Trust in Auditors' Going Concern Assessments?
Authors: Ahn Jaehan , Akamah Herita 
Published: December 2021

SUMMARY
Amidst heightened concern among U.S. and international regulators is the need to examine reasons why auditors are not issuing going-concern opinions (GCOs) to financially distressed clients who seem to warrant such opinions. We examine societal trust as one such reason, finding a lower incidence of GCOs with high societal trust. Moreover, we find that high societal trust is associated with fewer GCO Type I misclassifications, but more GCO Type II misclassifications. In addition, the association between societal trust and GCOs does not disappear for severely distressed clients, suggesting that auditors do not adequately perceive clients that warrant GCOs when the clients are in high trust countries, and illuminating a dark side to societal trust. Moreover, low litigation risk and auditor-management relationship longevity exacerbate this dark side of societal trust. Our study highlights how societal trust can have beneficial effects across multiple economic contexts while posing problems in the auditing context.
Data Availability: All data used in the study are available from the sources cited in the text.
JEL Classifications: D91; M41; M42.
Effect of Intrapapillary Local Anesthetic on Postoperative Pain Following Dental Treatment Under General Anesthesia in Pediatric Patients
Authors: Lipp Kelly , Casamassimo Paul , Griffen Ann , Smiley Megann , Bryant Jason , Draper John , Kumar Ashok 
Published: December 2021

Objective:
This randomized, prospective, blinded study compared pain in children following dental treatment under general anesthesia (GA) using 1 of 2 established analgesia methods.

Methods:
Patients age 4 to 7 years were randomly assigned to a control group (intravenous [IV] analgesics) or experimental group (IV analgesics and intrapapillary local anesthetic infiltrations) between July 2017 and February 2018. During recovery from surgery, Faces, Legs, Activity, Cry, and Consolability (FLACC) scores were recorded upon regaining consciousness and reassessed every 15 minutes until discharge. Overall pain occurrence (FLACC ≥1) and moderate/severe pain occurrence (FLACC ≥4) were analyzed using mixed effects logistic regression (N = 88).

Results:
The experimental group had a 17% lower overall pain occurrence than the control group (16 vs 33%; p = .02). Moderate/severe pain occurrence between the groups was not significant (9 vs 22%; p = .23). The dental treatment subjects received (number of completed stainless steel crowns, extractions, and/or pulpotomies) did not significantly affect pain occurrence.

Conclusion:
Local anesthesia intrapapillary infiltrations around stainless steel crowns decrease overall pain occurrence but not moderate/severe pain occurrence following dental treatment under GA in pediatric patients.
Programs and interventions promoting health equity in LGBTQ2+ populations in Canada through action on social determinants of health
Authors: Higgins Robert , Hansen Brian , Jackson Beth E. , Shaw Ashley , Lachowsky Nathan J. 
Published: December 2021

Abstract

Sexual and gender minorities (SGM) experience a number of health inequities. That social determinants of health drive these inequities is well-documented, but there is little evidence on the number and types of interventions across Canada that address these determinants for these populations. We conducted an environmental scan of programs in Canada that target SGM, and classified the programs based on their level of intervention (individual/interpersonal, institutional and structural). We found that few programs target women, mid-life adults, Indigenous people or ethnoracial minorities, recent immigrants and refugees, and minority language speakers, and few interventions operate at a structural level.
Comparative assessment of amateur fishing influence on water bioresources of Lake Tsatsa in Volgograd region
Authors: Kutsenko Nikolai Vladimirovich , Grozesku Yulia Nikolaevna , Filipenko Anton  Alexeyevich 
Published: December 2021

The paper presents the results of research conducted in 2020 on the impact of amateur fishing on the aquatic bioresources of Lake Tsatsa, which is part of the Sarpin Lakes located in the Volgograd region. On the basis of field studies (direct accounting), the number of amateur fishermen who visited the lake, as well as the qualitative and quantitative composition of their catches, was established. According to the data obtained, the total catch of fish by amateur fishermen, as well as the average catch per fisherman, was estimated. The size and weight composition of amateur fishing catches was determined based on the analysis of 2912 fish species. During the observation period 9 fish species were marked as part of amateur fishermen’s catches. The assessment of compliance of aquatic biological resources catch by amateur fishermen with the norms of the current Fishing Regulations was carried out. An assessment of the scale of amateur fishing impact on the aquatic bioresources of Lake Tsatsa is given, according to the analysis of the collected data. The catch of amateur fishermen in 2020 in Lake Tsatsa is amounted to 8.8232 tons, which can
account for up to 15% (about 65-70 tons) of the total stock of commercial fish species in the lake. The forecast values of the production volume for industrial fisheries, for which the total allowable catch (TAC) is established and the species for which the recommended catch is determined, are 5.9 and 18.6 tons, respectively. Out of the forecast volumes amateur fishermen additionally catch fish species for which the total allowable catch (TAC) is established (carp, bream, pike)
in the amount of 2.858 tons, and species for which the recommended catch value is determined as 5.643 tons.
Premature death in dogs with nontraumatic hemoabdomen and splenectomy with benign histopathologic findings
Authors: Millar Stephen L. , Curley Taylor L. , Monnet Eric L. , Zersen Kristin M. 
Published: December 2021

Abstract

OBJECTIVE
To determine whether premature death occurred among dogs with nonmalignant splenic histopathologic findings after splenectomy for nontraumatic hemoabdomen.

ANIMALS
197 dogs with nontraumatic hemoabdomen that underwent splenectomy and histopathologic evaluation between 2005 and 2018.

PROCEDURES
Information was obtained from electronic medical records, dog owners, and referring veterinarians to determine patient characteristics, histopathologic findings, survival information, and cause of death. Dogs were grouped based on histopathological diagnosis and outcome, and median survival times (MSTs) and risk factors for death were determined.

RESULTS
Histopathologic findings indicated malignancy in 144 of the 197 (73.1%) dogs with nontraumatic hemoabdomen. Hemangiosarcoma was diagnosed in 126 dogs (87.5% of those with malignancies and 64.0% of all dogs). Nine of 53 (17%) dogs with nonmalignant histopathologic findings had an adverse outcome and premature death, with an MST of 49 days. Risk factors for this outcome included low plasma total solids concentration, an elevated hemangiosarcoma likelihood prediction score, and a medium or high hemangiosarcoma likelihood prediction score category.

CONCLUSIONS AND CLINICAL RELEVANCE
This study showed that there is a group of dogs with nontraumatic hemoabdomen due to splenic disease that have nonmalignant histopathologic findings after splenectomy, but nonetheless suffer an adverse outcome and die prematurely of a suspected malignancy. Further evaluation of potential at-risk populations may yield detection of otherwise overlooked malignancies.
Single-injection caudal thoracic paravertebral block improves pain control and recovery quality in female dogs undergoing unilateral radical mastectomy: a randomized controlled trial
Authors: Santoro Francesco , Debidda Pasquale , Franci Paolo 
Published: December 2021

Abstract

OBJECTIVE
To test clinical and analgesic effects of a single-injection caudal thoracic paravertebral block (TPVB) after localization of the thoracic paravertebral space with a loss-of-resistance to air injection technique in female dogs undergoing unilateral radical mastectomy.

ANIMALS
14 client-owned dogs.

PROCEDURES
Dogs were premedicated with methadone, anesthetized with propofol and sevoflurane, and randomly assigned to receive a TPVB or no block preoperatively. Rescue analgesia with fentanyl and methadone was provided on the basis of cardiovascular responses during surgery and postoperative pain scores assigned with a validated pain scale. Required dose of rescue opioids; mean end-tidal sevoflurane concentration; episodes of hypotension, bradycardia, and other complications; quality of recovery scores; and postoperative pain scores were compared between groups.

RESULTS
Median intraoperative fentanyl doses were 0 µg/kg (range, 0 to 2 µg/kg) and 4 µg/kg (range, 2 to 6 µg/kg) for the TPVB and control groups, respectively. Median postoperative methadone doses were 0 mg/kg (range, 0 to 0.2 mg/kg) and 0.6 mg/kg (range, 0.4 to 0.6 mg/kg) for the TPVB and control groups, respectively. Recovery scores and pain scores assigned at the time of and 1 hour after extubation were significantly lower in the TPVB group than in the control group.

CONCLUSIONS AND CLINICAL RELEVANCE
A single-injection caudal TPVB improved pain control and recovery quality in female dogs undergoing unilateral radical mastectomy. Because the TPVB involves only a single injection, does not take long to perform, and requires only readily available low-cost equipment, the technique may be a valuable option in both referral and first-opinion practice.