bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients with COPD: Exploring patients’ coping ability during an interdisciplinary pulmonary rehabilitation programme: A qualitative focus group study
Authors: Abrahamsen Caroline Steen , Lang‐Ree Heidi Mandt , Halvorsen Kristin , Stenbakken Cesilie Meling 
Published: February 2021

AbstractAims and objectivesTo investigate experiences that contribute to enhancing patients’ ability to cope with COPD during interdisciplinary in‐hospital pulmonary rehabilitation programmes.BackgroundPatients with COPD often experience difficulties accepting their life situation as well as reduced levels of activity and social interaction. Despite the large body of research conducted on interdisciplinary collaboration and coping, few studies have examined patient perspectives of why and how pulmonary rehabilitation actually contributes to helping patients with COPD to cope with their illness.DesignQualitative design.MethodsThe data consist of qualitative focus group interviews with 17 participants, divided into three focus groups. The data were analysed using a thematic analysis approach. The Consolidated Criteria for Reporting Qualitative Research (COREQ) checklist was used.ResultsThe patients described a lack of knowledge and understanding of their illness. In addition, they felt that their illness imposed limitations on their lifestyle and social interaction. Support from healthcare professionals during pulmonary rehabilitation proved to be vital, yet the lectures and consultations they received were described as general and sometimes rushed. The patients reported a positive effect from undergoing pulmonary rehabilitation, but a loss of motivation after returning home.ConclusionThe findings indicate that gaining better health competence during pulmonary rehabilitation contributes to enhancing patients’ coping ability. Moreover, patients found it valuable to meet people in a comparable situation and to feel respected by healthcare professionals. Nevertheless, taking an individual empowerment‐oriented approach and focusing on the return home seem to be significant aspects of the patients’ improved and lasting ability to cope after pulmonary rehabilitation.Relevance to clinical practicePatients with COPD should be offered pulmonary rehabilitation because it has proven to have a positive effect on coping ability. Different follow‐up measures are vital after pulmonary rehabilitation to maintain motivation after returning home.
Accuracy of Intraoral Scanners for Recording the Denture Bearing Areas: A Systematic Review
Authors: Rasaie Vanya , Abduo Jaafar , Hashemi Saloumeh 
Published: February 2021

AbstractPurposeTo systematically review clinical and laboratory studies that investigated the accuracy of intraoral scanners in recording denture bearing areas.Materials and methodsElectronic and manual searches were conducted to identify all the available clinical and laboratory studies reporting the accuracy of digital impressions for recording denture related soft tissues. After the application of predetermined inclusion and exclusion criteria, the final list of articles was reviewed to meet the objective of this study.ResultsThe inclusion criteria were met by 18 studies out of which 8 were clinical and the rest were laboratory investigations. The eligible studies assessed the accuracy of intraoral scanners in recording both the denture supporting structures and the peripheral mobile tissues. The accuracy results were different among the various intraoral scanners. Likewise, the effect of several influencing factors, such as artificial markers, scanner head size, scanning strategy, and the operator's experience, were evaluated.ConclusionWhile the accuracy of intraoral scanners was comparable to the conventional techniques in recording bony structures with attached mucosa, they were not capable of accurately registering the mobile tissues. In addition, factors such as presence of a marker, larger scanner head size and specific scanning techniques appeared to improve the accuracy of the digital impression.
A reverse transcription loop‐mediated isothermal amplification assay for the detection of strawberry mottle virus
Authors: Ren Junda , Wang Jia , Zhu Yadong , Han Chenggui , Shang Qiaoxia 
Published: February 2021

AbstractStrawberry mottle virus (SMoV) is one of the most important viruses infecting strawberry all over the world, seriously affecting the yield and quality of strawberry especially in the occasion of compound infection. Loop‐mediated isothermal amplification (LAMP) as a new method of nucleic acid amplification has the advantages of rapid detection, simple operation and high sensitivity. Therefore, four specific LAMP primers using the 3′ end conservative coat protein (CP) gene were selected and designed according to the RNA2 sequence of SMoV obtained in NCBI GenBank. Results showed that the optimized RT‐LAMP reaction conditions including 1.0 µmol/L1 primers FIP/BIP, 0.1 µmol/L1 primers F3/B3, 2 mmol/L1 Mg2+, 1.2 mmol/L1 dNTPs, 1.0 mol/L1 Betaine and 2 mol/L1 DTT, with an optimal temperature set at 62°C for 45 min. The amplicons were successfully inspected by visual assessment using SYBR Green I as well as electrophoresis on agarose gel. The sensitivity of the LAMP was 100 times higher than the RT‐PCR method. Furthermore, it was highly specific for SMoV and showed no cross‐reaction with several other strawberry viruses. This assay will provide a practical method for rapid detection of large quantities of field samples and be useful in reducing the virus incidence through production of virus‐free planting material.
The Vietnamese Version of the Gratitude Questionnaire (GQ) and the Revised Short Gratitude, Resentment, and Appreciation Test (S‐GRAT): Psychometric Properties Among Adolescents
Authors: Tran Tu Anh Thi , Nguyen Phuoc Cat Tuong , Dinh Hong Van Thi , Nguyen Vinh Tuan 
Published: February 2021

AbstractThere is a lack of validated instruments to measure gratitude in Vietnamese adolescents; this issue is regarded as a significant impediment to progress in implementing empirical research on the gratitude of Vietnamese adolescents. The aim of this study, therefore, was to validate the Vietnamese translation of the Gratitude Questionnaire (GQ) and the Revised Short Gratitude, Resentment, and Appreciation Test (S‐GRAT), using two independent samples of Vietnamese adolescents in two studies. In Study 1, Sample 1 (N = 365, female n = 173) completed the Vietnamese GQ (GQ‐VN) and S‐GRAT (S‐GRAT‐VN). Confirmatory factor analysis showed that the 5‐item version of the GQ‐VN and the 15‐item version of the S‐GRAT‐VN fitted the data adequately. Both instruments showed satisfactory internal consistency. In Study 2, Sample 2 (N = 202, female n = 93) completed the GQ‐VN and S‐GRAT‐VN, along with the Satisfaction With Life Scale. The GQ‐VN and S‐GRAT‐VN were found to be positively correlated with each other and with the construct of life satisfaction, confirming the convergent and predictive validity of the two questionnaires. All the findings support the validity and reliability of the Vietnamese GQ and S‐GRAT in a sample of Vietnamese adolescents. These two instruments, therefore, may facilitate the evaluation of factors associated with adolescent dispositional gratitude in Vietnamese settings.
Use of healthcare resources and drug expenditure before and after treatment of chronic hepatitis C with direct antiviral agents
Authors: Vergara Mercedes , Miquel Mireia , Vela Emili , Cleries Montserrat , Pontes Caridad , Prat Alba , Rué Montse 
Published: February 2021

AbstractThe aim of this study was to analyse the impact of treating chronic hepatitis C (CHC) with direct‐acting agents (DAA) on the use of healthcare resources. We included all patients treated with DAA for CHC from January 2015 to December 2017 in Catalonia whose medical records from 12 months before to 24 months after treatment were available. Data were obtained from the Catalan Health Surveillance System. A total of 12,199 patients in Catalonia were treated with DAA for CHC. Of these, 11.3% had no‐minimal fibrosis (F0‐F1), 24.0% had moderate fibrosis (F2), 50.3% had significant fibrosis or cirrhosis (F3‐F4), and 14.4% had decompensated cirrhosis. Use of healthcare resources decreased from the pre‐treatment period to the post‐treatment period for the following: hospital admissions due to complications of cirrhosis, from 0.19 to 0.12 per month per 100 patients (RR 0.57; 95% CI 0.47–0.68); length of hospital stay, from 12.9 to 12.2 days (RR 0.93; 95% CI 0.91–0.94); outpatient visits, from 65.0 to 49.2 (RR 0.75; 95% CI 0.74–0.75); and number of medication containers per patient per month, from 13.9 to 12.5 (RR 0.837; 95% CI 0.835–0.838). However, the number of invoices for antineoplastic treatment increased after DAA treatment, especially for patients with high morbidity or advanced fibrosis stage. In conclusion, a decrease in health resource use was seen in CHC patients treated with DAA, as measured by length of hospital stay, number of admissions due to cirrhosis complications, outpatient visits and overall drug invoicing. However, use of antineoplastic drugs increased significantly, especially in patients with cirrhosis and high morbidity.
Clinical outcomes of systemic therapy in patients with unresectable or metastatic combined hepatocellular‐cholangiocarcinoma
Authors: Kim Eo Jin , Yoo Changhoon , Kang Hyo Jeong , Kim Kyu‐Pyo , Ryu Min‐Hee , Park Sook Ryun , Lee Danbi , Choi Jonggi , Shim Ju Hyun , Kim Kang Mo , Lim Young‐Suk , Lee Han Chu , Ryoo Baek‐Yeol 
Published: February 2021

AbstractBackground & AimsThe optimal systemic chemotherapy for combined hepatocellular‐cholangiocarcinoma (cHCC‐CCA) has not yet been defined. The definition and classification of cHCC‐CCA has changed recently in the 5th edition of WHO classification. We reviewed the pathological findings with the new classification and analysed the efficacy of systemic chemotherapy in patients with unresectable/metastatic cHCC‐CCA.MethodsAmong 254 patients with histologically confirmed cHCC‐CCA from 1999 to 2015 in Asan Medical Center, Seoul, Korea, 99 patients who received systemic chemotherapy for unresectable/metastatic disease were included. Overall response rate (ORR), progression‐free survival (PFS) and overall survival (OS) were retrospectively evaluated.ResultsSorafenib (n = 62) and cytotoxic chemotherapy (n = 37) were administered as first‐line chemotherapies; the ORR was 14.1%, and the median PFS and OS were 3.8 and 10.6 months, respectively, with a median follow‐up duration of 39.6 months. The efficacy outcomes were not significantly different between patients who received sorafenib and those who received cytotoxic chemotherapy (ORR, 9.7% vs 21.6%, P = .14; median PFS, 4.2 vs 2.9 months, P = .52; median OS, 10.7 vs 10.6 months, P = .34). In multivariate analysis, large intrahepatic tumour burden (≥30% of liver volume), elevated serum bilirubin and non‐platinum containing first‐line chemotherapy remained as significant prognostic factors for poorer OS.ConclusionsThe efficacy outcomes according to first‐line treatment were not significantly different between sorafenib and cytotoxic chemotherapy, and pathological findings were not found to help for determining appropriate therapeutic agent or assessing the prognosis. To overcome the poor treatment outcomes, further studies are needed to find proper treatment targets, biomarkers and the best treatment strategies.
Twelve‐month post‐treatment parameters are superior in predicting hepatocellular carcinoma in patients with chronic hepatitis B
Authors: Ahn Sang Bong , Choi Jun , Jun Dae Won , Oh Hyunwoo , Yoon Eileen L. , Kim Hyoung Su , Jeong Soung Won , Kim Sung Eun , Shim Jae‐Jun , Cho Yong Kyun , Lee Hyo Young , Han Sung Won , Nguyen Mindie H. ,   SAINT cohort
Published: February 2021

AbstractBackground & AimsThere are currently several prediction models for hepatocellular carcinoma (HCC) in chronic hepatitis B (CHB) receiving oral antiviral therapy. However, most models are based on pre‐treatment clinical parameters. The current study aimed to develop a novel and practical prediction model for HCC by using both pre‐ and post‐treatment parameters in this population.MethodsWe included two treatment‐naïve CHB cohorts who were initiated on oral antiviral therapies: the derivation cohort (n = 1480, Korea prospective SAINT cohort) and the validation cohort (n = 426, the US retrospective Stanford Bay cohort). We employed logistic regression, decision tree, lasso regression, support vector machine and random forest algorithms to develop the HCC prediction model and selected the most optimal method.ResultsWe evaluated both pre‐treatment and the 12‐month clinical parameters on‐treatment and found the 12‐month on‐treatment values to have superior HCC prediction performance. The lasso logistic regression algorithm using the presence of cirrhosis at baseline and alpha‐foetoprotein and platelet at 12 months showed the best performance (AUROC = 0.843 in the derivation cohort. The model performed well in the external validation cohort (AUROC = 0.844) and better than other existing prediction models including the APA, PAGE‐B and GAG models (AUROC = 0.769 to 0.818).ConclusionsWe provided a simple‐to‐use HCC prediction model based on presence of cirrhosis at baseline and two objective laboratory markers (AFP and platelets) measured 12 months after antiviral initiation. The model is highly accurate with excellent validation in an external cohort from a different country (AUROC 0.844) (Clinical trial number: KCT0003487).
Virus‐induced phytohormone dynamics and their effects on plant–insect interactions
Authors: Pan Li‐Long , Miao Huiying , Wang Qiaomei , Walling Linda L. , Liu Shu‐Sheng 
Published: February 2021

SummaryAttacks on plants by both viruses and their vectors is common in nature. Yet the dynamics of the plant–virus–vector tripartite system, in particular the effects of viral infection on plant–insect interactions, have only begun to emerge in the last decade. Viruses can modulate the interactions between insect vectors and plants via the jasmonate, salicylic acid and ethylene phytohormone pathways, resulting in changes in fitness and viral transmission capacity of their insect vectors. Virus infection of plants may also modulate other phytohormones, such as auxin, gibberellins, cytokinins, brassinosteroids and abscisic acid, with yet undefined consequences on plant–insect interactions. Moreover, virus infection in plants may incur changes to other plant traits, such as nutrition and secondary metabolites, that potentially contribute to virus‐associated, phytohormone‐mediated manipulation of plant–insect interactions. In this article, we review the research progress, discuss issues related to the complexity and variability of the viral modulation of plant interactions with insect vectors, and suggest future directions of research in this field.
The impact of surgical cancellations on children, families, and the health system in an Australian paediatric tertiary referral hospital
Authors: Drake‐Brockman Thomas F. E. , Chambers Neil A. , Sommerfield David , von Ungern‐Sternberg Britta S. 
Published: February 2021

AbstractBackgroundReasons for elective surgery cancelations and their impact vary from one institution to another. Cancelations have emotional and financial implications for patients and their families. Our service has a particularly broad and geographically diverse patient population; hence, we sought to examine these impacts in our service.MethodsWe identified families with procedural cancelations and administered a telephone questionnaire. Survey items included the reason for and timing of cancelation, how the family was informed, the mode of transport and distance traveled to the hospital, associated leave from work, expenses, whether the child was required to fast, missed school, as well as the child's and parent's emotional responses to the cancelation, along with overall parental satisfaction with how the cancelation was handled.ResultsDuring our study period, a total of 7870 procedures were booked. 6734 (86%) of these were completed and 1136 (14%) were canceled, with 6% canceled on the day of surgery. In 750 (66%) of these cancelations, families were successfully contacted by telephone and agreed to participate. Of these 305 (41%) cancelations were family‐initiated and 444 (59%) were hospital‐initiated, with these hospital‐initiated cancelations occurring closer to scheduled surgery. The most common cause of cancelation was that the child could not undergo the procedure due to illness (22%) or being unable attend the hospital (14%). The greatest disruption to families and children occurred when procedures were canceled late, particularly when the cancelation occurred on the day of the planned procedure.
Potential of rutabaga (Brassica napus var. napobrassica) gene pool for use in the breeding of hybrid spring Brassica napus canola
Authors: Shiranifar Bijan , Hobson Neil , Kebede Berisso , Yang Rong‐Cai , Rahman Habibur 
Published: February 2021

AbstractThe use of rutabaga for increasing the level of heterosis in spring canola was investigated. For this, test hybrids were produced by crossing four inbred populations, derived from F2 and BC1 of two rutabaga × spring canola crosses, to their spring canola parent, and were tested in field‐plots for agronomic and seed quality traits. Average mid‐parent heterosis (MPH) in these test hybrid populations was about 5%‐15% for yield. Compared to the F2‐derived population, the BC1‐derived population exhibited 1.8‐times greater MPH for yield. No correlation was found between inbred and test hybrid for yield; however, a positive correlation of genetic diversity of the inbred lines was found with MPH (r = .58) and hybrid yield (r = .36) suggesting that non‐additive effect of the genes may play an important role for high yield in hybrids. About 2% negative MPH was found for days to flowering, while almost no heterosis was found for seed oil and protein contents. Thus, our study demonstrates the potential of the rutabaga gene pool for use in hybrid canola breeding.
Inherited genetic late‐onset erythropoietic protoporphyria: A systematic review of the literature
Authors: Noyman Yehonatan , Edel Yonatan , Snast Igor , Sherman Shany , Kaftory Ran , Lapidoth Moshe , Mimouni Daniel , Hodak Emmilia , Levi Assi 
Published: February 2021

AbstractBackgroundInherited genetic erythropoietic protoporphyria (EPP) is characterized by a photosensitive rash that emerges during infancy or early childhood. Acquired EPP can erupt at any age, even during adulthood, and is associated with hematological disorders. A third, less‐studied type of EPP is also inherited but appears later in life (during adulthood).PurposeTo evaluate the characteristics of inherited genetic late‐onset (IGLO) EPP.MethodsA systematic comprehensive search of the literature was conducted using PubMed, Google Scholar, ScienceDirect, and clinicaltrials.gov databases. Studies describing patients with IGLO EPP were included. Additionally, we present an index case of a patient, treated at our clinic in whom inherited genetic EPP was diagnosed at age 21 years.ResultsThe search yielded 1514 citations. Five publications were eligible for review. Along with our case, 7 patients (4 males) were included in the analysis. Mean age at disease onset was 34.2 years (range 18‐69, median 30). Most patients presented with mild pruritus and rash in a photosensitive distribution. Mean level of free erythrocyte protoporphyrin IX (FEP) was 8.6 μmol/L. A mutant ferrochelatase gene (FECH) in trans to a hypomorphic FECH allele was found in 3 of the 4 patients who underwent genetic testing.ConclusionWe describe the distinct features of IGLO EPP. This work emphasizes that a diagnosis of inherited genetic EPP should not be ruled out in adults with new‐onset photosensitive manifestations.
Treatment of dental caries in a patient with Joubert syndrome without the use of sedatives: A case study
Authors: Oda Yuki , Yoshida Keita , Furutani Chiaki , Wakita Atsuko , Hama Yoko , Miyauchi Miwa , Okada Yoshiyuki 
Published: February 2021

AbstractBackgroundJoubert syndrome (JS) is a rare autosomal recessive inherited ciliopathy caused by gene mutation. Manifestations can include intermittent dyspnea, apnea, ataxia, and other nervous system abnormalities.Case presentationThe patient was a 21‐year‐old female with JS, severe intellectual disability, cerebral palsy, and epilepsy. Dental caries in both mandibular lower first molars was diagnosed by a local dentist, and the patient was referred to us for further treatment. Although her oral hygiene was good, the dental caries had reached the vicinity of the dental pulp. The caries had developed symmetrically on both first molars and was completely covered with the gingiva. As she was hesitant to receive treatment without anxiolysis, we considered using general anesthesia or intravenous sedation. However, we were recommended against it by the patient's family doctor because of the risk of apneic episodes in JS. Accordingly, dental treatment was performed over multiple days through physical behavior adjustment, while providing oxygenation (3 L/min) in preparation for an apneic episode. The dental treatment was successfully completed with a good postoperative outcome.ConclusionsDental treatment can be safely performed in patients with JS through physical behavior adjustment, thereby minimizing the risk of an apnea attack.
DBA/1 mice display equivalent cardiac function to C57BL/6J mice
Authors: Wang Zhen , Zhou Zhengwei , Guo Paipai , Wang Manman , Sun Hanfei , Tai Yu , Xiao Feng , Han Yongsheng , Wei Wei , Wang Qingtong 
Published: February 2021

New Findings

What is the central question of this study?
Do normal adult DBA/1 mice have cardiac function and performance equal to those of C57BL/6J mice?

What is the main finding and its importance?
Male adult DBA/1 mice show equivalent cardiac function to C57BL/6J mice up to 8 months old. Therefore, cardiac dysfunction could be investigated in an autoimmune diseases model established with DBA/1 mice.

AbstractCardiovascular mortality has been increasing, and in particular, cardiovascular damage caused by some chronic autoimmune diseases accounts for a large proportion of this. C57BL/6J mice have been used mostly in studies of cardiovascular diseases. However, for purposes of modelling, this strain of mouse has a very low incidence of some chronic immune diseases such as rheumatoid arthritis, to which instead DBA/1 mice are more susceptible. Basic cardiac function differs between mice with different genetic backgrounds. Therefore, we monitored cardiac function and structure of normal male C57BL/6J and DBA/1 mice for six consecutive months. Echocardiography was used to monitor cardiac functions once a month and cardiac systolic function was measured upon isoproterenol challenge at the end of observation. The Excitation–contraction coupling‐related proteins were measured by western blotting. Heart tissue sections were subject to haematoxylin–eosin, TUNEL and Alizarin red staining. The results demonstrated that systolic and diastolic function did not vary significantly and both strains were indistinguishable in appearance and structure of hearts. DBA/1 mice showed a good cardiac β‐adrenergic response comparable to C57BL/6J mice with isoproterenol treatment. The phosphorylation of phospholamban at either its protein kinase A or its Ca2+/calmodulin‐dependent protein kinase II site, as well as the activation of troponin I showed no significant difference between strains. These findings suggested that there was no obvious difference in the heart structure and function of normal male DBA/1 mice compared with C57BL/6J mice. The DBA/1 mouse is a strain applicable to investigating autoimmune disease‐induced heart dysfunction and exploring potential interventions.