bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of TNFAIP8 on the immune function of Th17 cells via p53/ p21/ MDM2 pathway after acute insult
Authors: Cheng Xiaobin , Wang Min , Li Jing , Li Gang 
Published: March 2021

Abstract
Background: Th17 cells induced immunosuppression plays a vital role during sepsis. Belonging to the tumor necrosis factor α induced protein 8 (TNFAIP8) family, TNFAIP8 associates with different immune cell physiopathological processes, thus its underlying regulatory mechanisms on Th17 cells in the acute insult processes have not been fully elucidated. Result: Sepsis was induced by cecal ligation and puncture (CLP) in the male adult C57BL/6 mice. The stable TNFAIP8 knockdown Th17 cells were established via lentiviral transfection with TNFAIP8-specific SiRNA . CCK-8 assay was conducted for measuring Th17 cell proliferation. Flow cytometric analysis was adopted for examining by flow cytometry. The p53/ p21/ MDM2 pathway was measured through western blot. As a result, high TNFAIP8 expression was related with acute insult and survival rate in septic mice. TNFAIP8 SiRNA reduced Th17 cell proliferation as well as cytokines production in vivo and in vitro. In addition, TNFAIP8 KD increased the Th17 cells apoptosis in WT and septic mice. Further, TNFAIP8 influences immune function of Th17 cell involving the p53/ p21/ MDM2 signaling. Actually, TNFAIP8 KD was suggested to regulate the up-regulation of P21 and MDM2, thereby increasing p53 protein expression during sepsis. P53 gene silencing contributed to reversing cell proliferation and apoptosis regulated by TNFAIP8 KD. Conclusion: Our work concluded that TNFAIP8 affected the immune function of Th17 cells, which is mediated via the p53/ p21/ MDM2 pathway after acute insult.
Dye adsorption revisited: Application of the cationic dye adsorption method for the quantitative determination of the acidic surface groups of nanocellulose materials
Authors: Araki Jun 
Published: March 2021

Abstract
The surface carboxyl and sulfate groups on cellulose nanowhiskers were quantified via the adsorption of toluidine blue O (TBO), a cationic dye. Here, simple and rapid protocols, such as mixing the nanowhisker suspensions with a dye solution, separating the supernatants via centrifugation, and determining the excess dye concentration via visible light absorbance techniques, were used to obtain reproducible results comparable with those obtained via titration. In addition to facilitating the discrete quantification of the sulfate and carboxyl groups, the TBO adsorption method enabled carboxyl quantification in the presence of mercapto groups, which was difficult to achieve via titration. The adsorption of TBO onto the carboxyl groups was completed within 30 min, enabling the rapid treatment of many samples within a short period.
Environmental DNA Reveals Arboreal Cityscapes at the Ancient Maya Center of Tikal
Authors: Lentz David L. , Hamilton Trinity L. , Dunning Nicholas P. , Tepe Eric J. , Scarborough Vernon L. , Meyers Stephanie A. , Grazioso Liwy , Weiss Alison A. 
Published: March 2021

Abstract
The city of Tikal, a major center of the ancient Maya world, has been the focus of archaeological research for over a century, yet the interactions between the Maya and the surrounding Neotropical forests remain largely enigmatic. To help fill that void, our study used a powerful new technology, environmental DNA analysis, which enabled us to characterize the site core vegetation associated with the artificial reservoirs that served as the city water supply. Because the area has no permanent water sources, these reservoirs were key to the survival of the city. In the absence of specific evidence, the nature of the vegetation surrounding the reservoirs has been the subject of scientific hypotheses for decades. To address these hypotheses we proceeded to capture homologous sequences of vascular plant DNA extracted from reservoir sediments using 120-bp genetic probes in a targeted enrichment approach. Samples date from the Early Preclassic period (1780 − 1620 BCE) to the Early Postclassic period (900–1100 CE), encompassing the time before, during and after the occupation of Tikal (1000 BCE-900 CE). Results indicate that during the Maya occupation the banks of the ancient reservoirs were primarily fringed with native tropical forest vegetation rather than domesticated species.
C1QTNF6 promotes Oral squamous cell carcinoma tumorigenesis though enhancing proliferation and inhibiting apoptosis of OSCC cells
Authors: Song Xiaobin , Li Longjie , Shi Liang , Liu Xinyu , Qu Xun , Wei Fengcai , Wang Ketao 
Published: March 2021

Abstract
BackgroundC1QTNF6 (CTRP6), a member of the CTRP family, has been recently implied to play a role in tumorigenesis. However, the expression status and the role of C1QTNF6 in oral squamous cell carcinoma (OSCC) remains unclear. MethodsImmunohistochemistry of OSCC tissue and data from TCGA both implied that C1QTNF6 was closely related to OSCC. We constructed lentivirus to knockdown C1QTNF6 in CAL27 cells and SCC-9 cells. Then the change of C1QTNF6 mRNA expression was detected with qRT-PCR, and the Western blot analysis was performed to detect changes in protein expression. Furthermore, Cell Cycle Analysis and Cell apoptosis analysis was measured. 4-week-old female BALB/c nude mice were purchased to observe the In vivo tumorigenicity. Finally, Pathway Analysis was performed.ResultsIn this study, we found that C1QTNF6 was overexpressed in OSCC tissues and cell lines, and the cellular proliferation was significantly decreased in C1QTNF6 knockdown OSCC cells. Knockdown of C1QTNF6 resulted in cell cycle arrest at the G2/M phase and enhanced apoptosis in OSCC cell lines. Further assays showed that C1QTNF6 silencing inhibits tumor growth of OSCC in vivo. Moreover, microarray analysis revealed that C1QTNF6 silencing results in significant alteration of many genes. Ingenuity Pathway Analysis (IPA) revealed that the Acute Phase Response signaling pathway was significantly activated following C1QTNF6 silencing. ConclusionsThese results suggested that C1QTNF6 play a promoting role in OSCC tumorigenesis, which may be a promising therapeutic target for OSCC treatment.
Hypoglossal Schwannoma Masquerading as a Submandibular Gland Tumor: a Case Report
Authors: Weng yanming , Li Sainan , Wu Hanjiang , Wu Kun 
Published: March 2021

Abstract
Background: Hypoglossal nerve schwannoma in the submandibular space is rare. This case report presents the treatment of a young patient affected by an unusual hypoglossal nerve schwannoma at the right side of the submandibular region. Case representation: A 31-year-old female presented to our department with complaints of a right-sided submandibular region mass. None calculi was observed by CBCT. An MRI of the neck demonstrating a 18×12mm mass located at the submandibular region. Based on clinical presentation and imaging, a diagnosis of a submandibular gland tumor was conferred and the patient scheduled for excision. Intraoperatively, the mass was noted to arise from the hypoglossal nerve, remaining independent of the submandibular gland. On histopathologic analysis, the mass was determined to be consistent with hypoglossal schwannoma. Conclusion: Though rare, the hypoglossal schwannoma should remain a consideration in the evaluation of a submandibular space mass. During operation, it might be better to explore the mass before managing the submandibular gland.
Assessment Of The Knowledge Of Palliative Medicine Among General Practitioners
Authors: Osowicka Magdalena , Wyszadko Anna , Mróz Piotr , Gorzewska Agnieszka 
Published: March 2021

Abstract
Background: Palliative patients who stay at home require exceptional medical care provided by General Practitioners (GPs) in conjunction with specialists in palliative medicine. To ensure effective treatment, proper cooperation between them is essential. Aim: An assessment of the knowledge and attitudes of GPs towards palliative medicine Design: The research was based on a survey questionnaire, consisting of 8 multiple choice questions. A total of participated anonymously in the survey. The data analysis included descriptive statistics and logistic regression. Participants: 83 GPs from the Pomeranian Region, 58 female and 25 male practitioners. Results: 59% of respondents defined palliative care as an activity that improves the quality of life of patients and their families facing problems related to a life-threatening illness. The coordination of the work of the palliative team was the most important task for the specialist in palliative medicine (58% of respondents). The majority stated that the most important activity was the prescription of painkillers (86%) and 84% found that being available during business hours was the most important. The next most important activity was delivering bad news (42% of respondents). Conclusions: Most of the GPs knew the correct definition of palliative care and the basics of pain management. Experienced physicians declared a lower willingness to be available during working hours and less often delivered bad news to terminal patients, compared to their younger colleagues. Individual treatment planning (98% of respondents) and reducing antibiotic therapy seem to be the most important aspects in pharmacotherapy.
Preparation and study of Z-type heterojunction composite photocatalytic material with Ag2MoO4&nbsp;modified rosette-like Bi2WO6 with Ag-SPR promoting effect
Authors: Gu Yufen , Bobo Guo 
Published: March 2021

Abstract
Here, the novel Ag 2 MoO 4 /Bi 2 WO 6 ( AMO/BWO ) heterojunction composite photocatalysts were synthesized by a two-step method (co-precipitation method + hydrothermal method) and characterized by a variety of characterization techniques including XRD, XPS, SEM, TEM, UV-vis DR spectroscopy, PL spectroscopy and FTIR spectroscopy, as well as EIS analyses, and photocurrent response for confirming the formation of Z-type heterojunctions. The generation and transfer of electron/holes in the AMO/BWO heterojunctions excited by simulated sunlight were analyzed. Under simulated sunlight, the photocatalytic performance of AMO/BWO composites were studied by degradation of organic pollutants. The experimental results show that the 30wt%AMO@BWO is the best photocatalytic composite material among all samples. Due to the prolonging of lifetime of the photoexcited electrons in the CB of AMO and holes in the VB of BWO, these carriers are more effective to participate in photodegradation reactions. An electron/hole transfer mechanism of the Z-scheme heterojunctions is proposed to illustrate the enhanced photodegradation performance of the AMO/BWO composite samples.
The static preload associated myogenic spontaneous fasciculation response in lengthening cardiac muscle
Authors: Fan Shouyan , Gao Lingfeng , Bell Annie Christel , Azure Joseph Akparibila , Wang Yang 
Published: March 2021

Abstract
The passive tension force enhancement is one kind of myogenic spontaneous fasciculation in muscles. However, its physiological properties in cardiac fibres are not well known. In this study, mice cardiac papillary muscle spontaneous force enhancement was evaluated by micro stepping stretch method. The occurrence of spontaneous force and real time cardiac fibre Ca2+ redistribution was tranced by Flou-3 (2mM) indicator. Force enhancement amplitude, enhancement prolonging time, and tension–time integral were analysis by myograph analyser. The results indicated that the spontaneous force occurred immediately after the active stretch, rapidly enhanced during tolerating the sustained static stretch. The force occurrence and amplitude enhance synchronized with the Ca2+ recruitment and lightning transmitted to adjacent fibres. In high preload fibres, the enhancement was forceful to over its maximum passive tension (6.20 ± 0.51 N/mm2 to 4.49 ± 0.43 N/mm2). The force occurrences were unsteadiness in each stretch. The increased enhancement amplitude combining with the shortening prolonging time induced reduction of tension–time integral. We concluded that the intracellular Ca2+ synchronized force enhancement is one kind of interruption event in overloading cardiac fibres. This interruption occurred during the relaxation processing in cardiac muscle, therefore affect the rhythmic stability of cardiac relaxation-contraction cycle.
Predictors of Knowledge on Kangaroo Mother Care Practice among Postnatal Mothers with Preterm Babies in Tanzania: A Hospital-Based Cross-Sectional Study
Authors: Chamhene Naomi , Moshi Fabiola Vincent 
Published: March 2021

Abstract
Background: There is slower progress on decreasing preterm deaths despite of essential newborn care intervention in Tanzania. About 24% of neonatal deaths in Tanzania occur due to prematurity related complications. However, there are limited studies which explain the knowledge level of postnatal mothers on KMC practice in Tanzania, especially in Central zone. Therefore, the purpose of this study was to determine predictors of knowledge on Kangaroo mother care among postnatal mothers of preterm babies in Central zone, Tanzania.Methods: The study design was an analytical cross sectional involved 363 mothers of preterm babies as participants from three Referral Regional Hospitals in Central zone. Convenience sampling technique was used to get participants in the study where by all postnatal mothers with premature babies who met the inclusion criteria were enrolled until the sample size was attained.Face to face interview using structured questionnaire was used to collect data. Data was analyzed by using SPSS v23. Both univariate and multiple regression analyses were used to determine predictors of knowledge Results: Majority of postnatal mothers 225(62%) had inadequate knowledge towards KMC. After controlling for confounders, predictors of knowledge on KMC were region a postnatal mother was residing [Singida (AOR=1.990 at 95% CI=1.058-3.741, p=0.033)], age of the mother [women aged ≥30 years(AOR=3.913 at 95% CI=1.038-14.75, p=0.044)], level of education of the mother [women who had secondary education and above (AOR=6.309 at 95% CI=2.305-17.26, p<0.001)] and the family type of a postnatal mother living in (nuclear family AOR=0.521 at 95%CI=0.313-0.866,p=0.012).Conclusion: Vast majority of postnatal mothers had inadequate knowledge on KMC practice which threatens the health of preterm babies who are discharged home before full development. Postnatal women who were more likely to have inadequate knowledge were those of young age, with low level of education and living in nuclear families. The study recommends care of preterm babies (including KMC) to be included in the antenatal training package in order to prepare them for the care if they gave birth to a preterm baby.
ALDH2 Polymorphism rs671 is A Predictor of Immune Checkpoint Inhibitor Efficacy Against Chest Malignancies
Authors: Matsumoto Akiko , Nakashima Chiho , Kimura Shinya , Sueoka Eizaburo , Aragane Naoko 
Published: March 2021

Abstract
Background

Aldehyde dehydrogenase 2 (ALDH2) plays an important role in the endogenous aldehyde detoxification of various types of cells. ALDH2*2, a variant allele of the ALDH2 polymorphism rs671, leads to decreased enzymatic activity. ALDH2*2 may enhance tumor antigen presentation due to aldehyde-induced DNA damage while suppressing peripheral blood T cell counts and T cell activation.
Methods

On the basis of our hypothesis that rs671 affects the sensitivity of immune checkpoint inhibitors (ICIs), we evaluated the effects of rs671 on patients with chest malignancies who started ICI therapy in 2016–2019. The cohort consisted of 105 cases, including 64 adenocarcinoma and 30 squamous cell carcinoma patients, 49 of whom were ALDH2*2 carriers. Nivolumab, Pembrolizumab, or Atezolizumab was used for the first ICI treatment.
Results

The best response to ICI therapy (partial response/stable disease/progressive disease) was 36%/50%/14% in the rs671(−) cases; however, the response was relatively poor in the rs671(+) cases (27%/29%/45%, respectively) (p = 0.002). The hazard ratio (95% confidence interval) of disease progression within the observation period of 6 months for the rs671(+) cases was estimated to be 5.0 (2.5–10) after the adjustment for covariates, including sex, Brinkman index, treatment line, tumor tissue programmed death-ligand 1 positivity rate, and tumor tissue EGFR mutation. This association was also maintained in a stratified analysis, suggesting that ALDH2*2 is an independent negative predictive factor for the short-term prognosis of ICI therapy. Thus, the progression-free survival (PFS) ratio of the rs671(+) cases decreased rapidly after ICI initiation but was eventually higher than that of the rs671(−) cases (restricted mean survival time in 12 months from 2 to 3 years afterward was 1.3 times that of the rs671(−) cases). Moreover, the highest PFS ratio after 2 years among sub-groups was found in the first-line treatment group of rs671(+) patients (40%).
Conclusions

Our study suggests that rs671 may be an accurate and cost-effective predictor of ICI treatment, in which optimal case selection is an important issue.
Influence of Angle Alpha on Visual Quality after Implantation of Extended Depth of Focus Intraocular Lenses
Authors: Qin Miaomiao , Ji Min , Zhou Tianqiu , Yuan Yurong , Luo Jiawei , Li Pengfei , Wang Ying , Chen Xiaojuan , Chen Wei , Guan Huaijin 
Published: March 2021

Abstract
Background: To assess postoperative changes in angle alpha, and to evaluate the postoperative visual quality of patients with different angle alpha values after implantation of extended depth of focus (EDOF) intraocular lenses (IOLs).Methods: Seventy-nine eyes of 79 patients who had phacoemulsification with EDOF IOLs implantation were enrolled. A cut-off value of 0.3 mm, 0.4 mm, and 0.5 mm in preoperative angle alpha was chosen to divide eyes into groups. Distance, intermediate, and near visual acuities, modulation transfer function (MTF), and aberrations were recorded during a 6-month follow-up. A patient questionnaire was completed. Results: There were no significant differences in angle alpha postoperatively compared to preoperatively. No significant differences were found in visual acuity and MTF between all groups. With 5mm pupil diameter, there were significant differences of higher-order aberrations and spherical aberration in ocular aberration and internal aberration between angle alpha＜0.4 mm and angle alpha≥ 0.4 mm. Additionally, significant differences of coma were also added in cut-off value of 0.5 mm. When the value of angle alpha is 0.4 mm or higher, there were significant differences in the score of halos and glare.Conclusions: Angle alpha did not affect visual acuity, but the value of 0.4 mm or higher in angle alpha affected the visual quality under scotopic conditions and occurrence of halos and glare. For patients with 0.4 mm or higher in angle alpha, the decision to implant a EDOF IOL should be carefully considered.
ATG7 Polymorphisms rs7625184 and rs2606750 are Not Associated with Parkinson’s Disease: A Case Control Study
Authors: Zou Ming , Wang Jian-Yong , Lv Ren-Jun , Nie Shan-Jing , Zhu Lan-Bing , Liu Xue-Ping 
Published: March 2021

Abstract
Background: Deregulation of autophagy is involved in the development and progression of Parkinson’s disease. ATG7, an E1 like enzyme, palys a key role in autophagy. This study aimed to investigating the association between ATG7 polymorphisms and PD susceptibility. Methods: Single nucleotide polymorphisms of ATG7, including rs7625184 and rs2606750, were identified by polymerase chain reaction-restriction fragment length polymorphism in a Han Chinese population consisting of 312 PD patients and 309 healthy controls. Results: Genotyping analyses showed that none of the 2 SNPs was significantly associated with PD risk.Conclusions: Our results suggest that rs7625184 and rs2606750 are not associated with PD susceptibility. Further studies are warranted in revealing the links between ATG7 and PD.
The Prognostic and Clinicopathological Significance of LASP1 Expression in Cancer Patients: Systematic Review and Meta-Analysis
Authors: Luo Jian , Zhu Yuanzhi , Long Ying , Huang Fei , Luo Xiaozou , Wang Jianhong , Hu Zhen , Miao Hongbin , Huang Yulan 
Published: March 2021

Abstract
Background

Many cancer researchers have investigated the prognostic significance of LASP1 for survival of patients with various types of cancer. Nevertheless, the role LASP1 palyed in cancer prognosis remains unknown. In consequence, we carried out this study in order to comprehensively analyze the prognostic value of LASP1 in cancer patients.
Methods

A systematical research was conducted in electronic databases, such as PubMed, Embase and Web of Science. Eighteen studies meeting the inclusion criteria were enrolled. Overall survival (OS), recurrence-free survival (RFS) and various clinicopathological parameters were used as the endpoints in this study.
Results

A total of 2023 cancer patients from eighteen studies were finally enrolled into our meta-analysis. The results revealed that the cancer patients with high expression of LASP1 exhibited shorter OS (HR = 2.04, 95%CI = 1.77–2.34, P < 0.01) and RFS (HR = 2.11, 95%CI = 1.51–2.95, P < 0.01) than those with low expression of LASP1, and patients whose tumors expressed high LASP1 had shorter OS in lung cancer (HR = 2.20, 95%CI = 1.45–3.36, P < 0.01) and gastric cancer (HR = 1.64, 95%CI = 1.14–2.36, P < 0.01) respectively. Furthermore, the cancer patients whose tumors expressed high LASP1 were apparently associated with advanced TNM stage (OR = 2.92, 2.27–3.76, P < 0.01), earlier lymph node metastasis (OR = 2.69, 1.62–4.45, P < 0.01), advanced T classification (OR = 2.17, 1.48–3.18, P < 0.01) and earlier distant metastasis (OR = 2.56, 1.03–6.35, P = 0.04) when compared to those whose tumors expressed low LASP1.
Conclusions

Our study showed that the high LASP1 expression might be an undesirable predictor for patients with various types of cancers in the aspect of OS, RFS, TNM stage, lymph node metastasis, T classification and distant metastasis, and the high LASP1 expression might be an undesirable predictor for lung cancer patients and gastric cancer patients. Therefore, the expression of LASP1 might be utilized as a novel indicator in judging the prognosis of cancer patients, especially in lung cancer and gastric cancer.
Nursing Management of Fatigue in Cancer Patients: Mixed Methods Study
Authors: Tolotti Angela , Bonetti Loris , Pedrazzani Carla , Bianchi Monica , Moser Laura , Pagnucci Nicola , Sari Davide , Valcarenghi Dario 
Published: March 2021

Abstract
BackgroundFatigue is a complex and frequent symptom in cancer patients, influencing their quality of life, but it is still underestimated in clinical practice and undertreated. The aims of this study were to detect the presence of fatigue in cancer patients, describe the perceptions of patients and nurses regarding it and describe the nursing management of fatigue.MethodsMixed methods study. Data were collected in two oncological wards using the Brief Fatigue Inventory (BFI), an ad hoc questionnaire, patient interviews, focus groups with nurses and the review of nursing records. Interviews and focus groups were analysed through thematic analysis. We used SPSS 22.0 for quantitative data and Nvivo 10 for qualitative data analysis.ResultsA total of 71 questionnaires were analysed (39 males, mean age 65,7(±14) years). Fatigue was reported 5 times (7%) in nursing records, while in 17 cases (23.9%) problems related to it were reported. Twelve patients were interviewed. Five themes were identified: feeling powerless and aggressive, my strategies or what helps me, feeling reassured by the presence of family members, feeling reassured by nurses’ gestures, being informed. Three themes were identified through the focus groups: objectivity and subjectivity in the assessment of fatigue, nursing contribution within the multidisciplinary management of fatigue, and difficulty in evaluating outcomes.DiscussionThe approach to the management of fatigue was unstructured. Patients were satisfied with the care received but needed more information and specific interventions. Useful aspects were identified that could be used to change the professional approach towards the management of fatigue.