bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic and Functional Impact of Perioperative LAＭA/LABA Inhaled Therapy In Patients With Lung Cancer and Chronic Obstructive Pulmonary Disease&nbsp;
Authors: Azuma Yoko , Sano Atsushi , Sakai Takashi , Koezuka Satoshi , Otsuka Hajime , Tochigi Naobumi , Isobe Kazutoshi , Sakamoto Susumu , Takai Yujiro , Iyoda Akira 
Published: February 2021

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is an important risk factor for postoperative complications and mortality. The utility of several perioperative bronchodilators in patients with COPD requiring surgery for lung cancer has been reported, but the most suitable agent and its specific effect on postoperative long-term prognosis remain unclear. To determine the effects of perioperative combination therapy, using a long-acting muscarinic antagonist (LAMA) and a long-acting β2 agonist (LABA), on preoperative lung function, postoperative morbidity and mortality, and long-term outcome in COPD patients.Methods: Between January 2005 and October 2019, 130 consecutive patients with newly diagnosed COPD underwent surgery for lung cancer. We conducted a retrospective review of their medical records. Patients were divided into 3 groups according to perioperative management: LAMA/LABA (n=64), LAMA (n=23) and rehabilitation only (no bronchodilator) (n=43). Results: Patients who received preoperative LAMA/LABA therapy showed significant improvement in lung function before surgery (p<0.001 for both forced expiratory volume in 1 second (FEV1) and percentage of predicted forced expiratory volume in 1 second (FEV1 %pred). Compared with patients who received preoperative LAMA therapy, patients with LAMA/LABA therapy had significantly improved lung function (ΔFEV1, 223.1 mL vs 130.0 mL, ΔFEV1 %pred, 10.8% vs 6.8%; both p<0.05). There was a trend toward a lower incidence of postoperative complications in the LAMA/LABA group compared with the LAMA and rehabilitation-only groups. In patients with moderate to severe air flow limitation (n=61), those who received LAMA/LABA therapy had significantly longer overall survival and disease-free survival compared with patients in the other groups. Perioperative LAMA/LABA therapy was also associated with lower recurrence rates. Conclusions: Patients who receive perioperative LAMA/LABA for moderate to severe COPD have improved prognosis and better pulmonary function with surgery for lung cancer. We believe this treatment combination is optimal for patients with lung cancer and COPD.
The Role of Vitamin D on the Prognosis and Incidence of Lung Cancer: A Systematic Review and Meta-Analysis
Authors: Qian Mingxia , Guo Dina , Fu Rongrong , Qi Shuping , Fu Xiaojun , Yuan Lingling , Qian Lilin 
Published: February 2021

Abstract
Background: The correlation between vitamin D intake and lung cancer development is controversial. This meta-analysis aims to evaluate the relationship between vitamin D and the prognosis and incidence of lung cancer. Methods: A comprehensive database search on Pubmed, Web of Science, EBSCO, and Cochrane Library was carried out from the beginning to November 2020. Long-term survival and the incidence rate of patients with lung cancer were the primary outcomes of the study. Results: Ten eligible studies were selected for the meta-analysis following specific inclusion and exclusion criteria. Four included studies, covering 5007 patients, compared the overall survival (OS) and relapse-free survival (RFS) of lung cancer patients among total vitamin D users with non-users. Significantly, the estimated pooled hazard ratio (HR) revealed that vitamin D could improve OS and RFS of lung cancer patients [HR=0.83, 95% CI (0.72-0.95); HR=0.79, 95% CI (0.61-0.97), respectively]. Vitamin D intake was inversely associated with lung cancer incidence in six studies [OR=0.90, 95% CI (0.83-0.97)]. Conclusions: The present meta-analysis shows vitamin D not only improves the long-term survival of lung cancer patients but has a beneficial effect on the incidence of lung cancer. Notwithstanding, more studies are needed to confirm the study results.
Mapping the functional brain state of a world champion freediver in static dry apnea
Authors: Annen Jitka , Panda Rajanikant , Martial Charlotte , Piarulli Andrea , Nery Guillaume , Sanz Leandro R. D. , Valdivia-Valdivia Juan M. , Ledoux Didier , Gosseries Olivia , Laureys Steven 
Published: February 2021

Abstract
Voluntary apnea showcases extreme human adaptability in trained individuals like professional free divers. We evaluated the physiological and psychological adaptation and the functional cerebral changes using EEG and fMRI to 6.5 minutes of dry static apnea performed by a world champion free diver. Compared to resting state at baseline, apnea was characterized by increased EEG power and functional connectivity in the alpha band, along with decreased delta band connectivity. fMRI connectivity was increased within the DMN and visual areas but decreased in pre- and postcentral cortices. While these changes occurred in regions overlapping with cerebral signatures of several meditation practices, they also display some unique features that suggest an altered somatosensory integration. As suggested by the self-reported phenomenology, these findings could reflect the ability of elite free divers to create a (functional) dissociation between the body and the mind when performing prolonged apnea.
Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts
Authors: Shabana Hatem Ahmed , Mahmoud Tamer , Gairola Sanjay , Ketbi Asma Al , Aljasmi Mariam , Sallani Mariam Al 
Published: February 2021

Abstract
Background

This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness.
Results

The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination.
Conclusions

Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.
Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy
Authors: Zhou Xinhai , Zhou Dawei , Bao Xinhui , Zhou Jie , Xin Fengxue , Zhang Wenming , Qian Xiujuan , Dong Weiliang , Jiang Min , Ochsenreither Katrin 
Published: February 2021

Abstract
Background: Recently, a newly isolated oleaginous yeast strain Scheffersomyces segobiensis DSM 27193 was identified with the capability of accumulating high contents of palmitoleic acid (POA) in its intracellular lipid (13.8%). However, high amounts of ethanol as the main by-product were also produced in this system. To eliminate ethanol formation and to further improve POA production, process optimization focused on dissolved oxygen (DO) regulation was conducted in this study.Results: As a result, agitation control was found to be highly beneficial for cell growth and lipid production. When keeping pO2>40% through dynamic agitation control, significant improvements toward lipid (70.12% increase) and POA (1.44 fold) production have been obtained, and the accumulation of ethanol was decreased to 2.3 g/L. While increasing the aeration rate to 2 vvm, no significant decrease has been found in ethanol accumulation. Further application of a two-stage agitation regulation (600 rpm in the first 48 h fermentation then adjusted the agitation speed to 1000 rpm) gave 10.23 g/L of lipid (50% increase) with 2.02 g/L of POA (1.15 fold) production. At last, under the best condition of 1000 rpm of agitation and 1 vvm of aeration, no ethanol was detected during the whole fermentation process, while a dry biomass concentration of 44.8 g/L with 13.43 g/L of lipid and 2.93 g/L of POA was achieved. Transcription analysis revealed that ethanol synthetic pathway was down regulated under the condition of high agitation, while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.Conclusion: This study introduces the oleaginous yeast chassis S. segobiensis DSM 27193 and an effective pO2 regulation strategy for efficient biological production of POA.
Methylobrevis albus sp. nov., isolated from freshwater lake sediment
Authors: Zou Qi-Hang , Feng Xi , Wang Tao-Jie , Du Zong-Jun 
Published: February 2021

Abstract
An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from freshwater of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30 ℃ in medium containing 0-0.75%(w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C16:1ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main ubiquinone was Q-10. The G + C content of genomic DNA was 69.8 mol%. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to M. pamukkalensis JCM 30229T with 95.9% sequences similarity. Based on taxonomic results in this study, we proposed that strain L22T was placed into a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (= KCTC 72858T = MCCC 1H00432T).
Internet Gaming Disorder: A Preliminary Exploration of Cultural Orientation Profiles
Authors: Stavropoulos Vasileios , Frost Tyler Michael John , Brown Taylor , Gill Peter , Kannis Lee Dymand 
Published: February 2021

Abstract
Background

Internet Gaming Disorder (IGD) involves excessive use of Internet games to the extent that one’s everyday life is compromised. It has been suggested that IGD symptoms are dependent on a person’s cultural orientation. However, the range of potential cultural orientation effects on IGD presentations remains largely unknown. The current study aims to further understanding of the role of cultural orientation in IGD, allowing us to develop more culturally responsive and gamer centred IGD prevention and intervention strategies.
Methods

One thousand and thirty-two participants with internet gaming experience were measured for IGD symptom’s severity using the Internet Gaming Disorder Scale – Short Form (IGDS9-SF) and cultural orientation behaviours via the Individualism & Collectivism Scale (ICS). Latent Class Analysis (LCA) and T-Tests were performed in relation to their responses.
Results

Upon inspection of the LCA output, two cultural profiles of internet gamers were identified. These included the Collectivism Aversive gamers (CA; 11%) and the Collectivism Neutral gamers (CN; 89%). The CA gamers displayed significantly higher IGD behaviours overall, and, were higher in preoccupation, withdrawal symptoms, tolerance, relapse, deception, escapism/mood modification, and functional impairment compared to CN gamers. There were no differences between CA and CN gamers in loss of interest and conflicts with others.
Conclusions

The findings suggest that cultural orientation can influence the presentation of IGD. Specifically, those who are less collectivist or less influenced by social groups display greater IGD symptoms and present a profile that requires a different intervention from gamers who are more collectivistic. Researchers and clinicians should emphasize the value of belonging in a collective and experiencing equality with others in relation to mental health and gaming patterns.
Multi-Objective Clustering Protocol for Heterogeneous Wireless Sensor Network
Authors: purkar san Vish , Deshpande Rajkumar S 
Published: February 2021

Abstract
As we know the capabilities of communication system seems to be extended to greater level with the integration of wireless sensor network (WSN).Seamless control of real time application is been possible. But, an energy constraint of sensor node limits the lifecycle of the activity. Thus, it is necessary to utilize the available energy efficiently to prolong the lifetime of sensory system. Secondly by adding Heterogeneity in WSN and by controlling the communication activity it is possible to extend the lifetime of the network multifold. Thus, it is possible to improve energy efficiency of WSN without constraints of deployments, responsibility and heterogeneity level. So, design perceptive based on multilevel clustering scheme for varied objectives is the better choice for proposed scheme. As clustering scheme reduces internal traffic, efforts of communication and improve the load balancing over the network. Here, we propose Multi-objective (MO)clustering protocol for Heterogeneous WSN to enhance the energy efficiency of deployed network. We hereby found that proposed scheme work better for varied deployment condition and for various performance parameters. Simulation results of proposed design outperform the well-known published protocols by more than 1.97 times.
Characterization of B-cell Receptor Heavy Chain Complementarity-determining Region 3 Repertoire Based on the Differences of Symbiotic Microorganisms in the Gut of Mice
Authors: Li Jun , Pan Yurong , Ma Qingqing , Ma Long , Shi Bin , Wang Xiaomei , He Xiaoyan , Yao Xinsheng 
Published: February 2021

Abstract
Background

Colonization of gut microorganism is related to maturation of B cells in peripheral immune organs. This study aims to investigate the effect of intestinal microflora in Germ-free (GF), Specific Pathogen-free (SPF) and Clean (CL) BALB/C mice to small intestine total B-cell and memory B-cell receptor (BCR) complementary-determining region 3 (CDR3) repertoire.
Results

The composition and characteristics of intestinal microflora were analyzed by 16S rDNA sequencing. Genomic DNA extracted from small intestine tissue and memory B-cells of GF, SPF and CL mice were conducted via high-throughput DNA sequencing methods. As expected, significant differences of gut microflora diversity were observed in the three mice groups. CL group showed the most diversity, followed by SPF group, and GF group had the lowest diversity. Moreover, anormogenesis of intestinal lymphoid tissue were observed in GF mice. Diversity of the BCR heavy chain CDR3 repertoire in memory B cells were significant difference among three groups, but not in total B cells. The nucleotide polymorphism, usage frequency of gene segments (V, D, J, V–J gene segments) and amino acid of total B cells and memory B cells CDR3 were comparable among three mice groups, and there was significant difference between CL and GF mice groups.
Conclusions

The results of this study advocate that the colonization of intestinal microorganisms affect the diversity of B cells CDR3 repertoire. Elucidating mechanism of microbiome participated in the function of intestinal mucosal immune system may have positive effects on human health, and it requires further investigation.
Structure, Electrical and Optical Properties of ITO Thin Films and Their influence on Performance of CdS/CdTe Thin-Film Solar Cells
Authors: Ahmed Moustafa , Bakry Ahmed , Shaaban Essam R , Dalir Hamed 
Published: February 2021

Abstract
ITO was prepared by mixing gradient In2O3 and SnO2 powders using solid phase reaction manner. Using electron beam gun tool, ITO films with varied thicknesses were fabricated. The structure, electrical and optical parameters of the prepared films were studied. XRD patterns were used to establish the micro-structural parameters (lattice strain and crystallite size). The SEM shows improvement of grain size with the increase of the film thickness. The electrical parameters of ITO films were measured by means of the standard four-point probe method. It was found that when the film thickness increases from 75 nm to 375 nm, the resistivity decreases to lower value of 1.65×10-4 Ω.cm and slightly increases to 1.93 ×10-4 Ω.cm at thickness of 375 nm. The ITO films with lower electrical properties are appropriate for high-efficiency CdTe solar cells. In terms of spectral ellipsomeric, three optical layer models (adhesive layer of the substrate/B-spline layer of ITO film/surface roughness layer) were applied to estimate the film thickness with high accuracy. The absorption coefficient and energy gap were calculated from the transmission and reflection spectra in the strong absorption region. As the film thickness increases, the optical energy gap was found to increase from 3.56 eV to 3.69 eV. In terms of Hall-effect measurements, both carrier concentration and hall mobility were determined. In addition, influences of ITO layers with various thicknesses on the performance of CdS/CdTe solar cells were checked. When the ITO window layer thickness is 325 nm, Jsc = 17 mA/cm2, Voc = 0.82 V, and FF = 57.4%, the calculated highest power conversion efficiency (PCE) is 8.6%.
Network Pharmacology-Based Approach to Investigate the Mechanisms of Piwei-Peiyuan Decoction in the Treatment of Gastric Carcinoma
Authors: Pei Bei , Wen Zi'ang , Zheng Yanmin , Li Xuejun 
Published: February 2021

Abstract
Purpose

Piwei-Peiyuan Decoction is a traditional Chinese medicine decoction, which has shown promising treatment in gastric carcinoma. However, the molecular mechanisms of gastric carcinoma (GC) have not been systematically revealed yet. In this work, a network pharmacology research was conducted to analyze mechanisms of Piwei-Peiyuan Decoction, aiming to provide a basis for the development and application of new drugs in treatment of GC.
Methods

In this study, a network pharmacology approach was used to predict targets, construct network maps and analyze relevant signaling pathways. We discovered active chemical ingredients and their targets in the Piwei-Peiyuan Decoction by the TCMSP and ADME database. Then we found main targets of GC by GeneCards (https://www.genecards.org), OMIM (http://www.omim.org) and DRUGBANK (https://go.drugbank.com) databases. According to the information of ingredients and GC, a PPI network was performed by the String (https://string-db.org) database. And a targets-pathways interaction network was constructed by Cytoscape (version 3.7.2), a bioinformatics software used for data visualization and integration.
Results

Our result indicated that the main active ingredients of Piwei-Peiyuan Decoction in treating GC are Isorhamnetin, Kaempferol, Quercetin and Luteolin, and main active targets are CASP-3, MAPK1, MYC, AKT1, P53. We found the mechanism of Piwei-Peiyuan Decoction for treating GC is mainly to regulate PI3K-Akt signaling pathway, MAPK signaling pathway, Ras signaling pathway, IL-17 signaling pathway, HIF-1α signaling pathway to inhibit cell proliferation, promote apoptosis and then exert anti-cancer effects.
Conclusion

This study preliminarily analyzed the multi-component, multi-target and multi-path mechanism of Peiwei-Peiyuan Decoction in the treatment of gastric carcinoma, providing a theoretical basis for its clinical application.
Arbuscular mycorrhizal fungi increase Pb uptake of colonized and non-colonized Medicago truncatula root and deliver extra Pb to colonized root
Authors: Zhang Haoqiang , Ren Wei , Zheng Yaru , Zhao Fei , Tang Ming 
Published: February 2021

Abstract

Aims
Arbuscular mycorrhizal (AM) fungi form symbiosis with terrestrial plants and improve lead (Pb) tolerance of host plants. The AM plants accumulate more Pb in root than their non-mycorrhizal counterparts. However, the direct contribution of the mycorrhizal pathway to host plant Pb uptake was less reported.
Methods
In this study, the AM fungi colonized and non-colonized root of Medicago truncatula was separated by a split-root system, and their differences in responding to Pb application was compared.
Results
Inoculation of Rhizophagus irregularis increased shoot biomass accumulation and transpiration, and decreased both colonized and non-colonized root biomass accumulation. Application of Pb in the non-colonized root compartment increased the colonization rate of R. irregularis and up-regulated the relative expressions of MtPT4 and MtBCP1 in the colonized root compartment. Inoculation of R. irregularis increased the Pb uptake in both colonized and non-colonized plant root, while R. irregularis transferred Pb to the colonized root. The Pb transferred through the mycorrhizal pathway had low mobility move from root to shoot, and might be sequestrated and compartmented by R. irregularis.
Conclusions
The Pb uptake of plant root might follow water flow that facilitated by the aquaporin MtPIP2. The quantification of Pb transfer via mycorrhizal pathway and the involvement of MtPIP2 deserve further study.