bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity in olfactory receptor repertoires is associated with dietary specialization in a genus of frugivorous bat
Authors: Yohe Laurel R , Leiser-Miller Leith B , Kaliszewska Zofia A , Donat Paul , Santana Sharlene E , Dávalos Liliana M 
Published: July 2021

Abstract
Mammalian olfactory receptor genes (ORs) are a diverse family of genes encoding proteins that directly interact with environmental chemical cues. ORs evolve via gene duplication in a birth-death fashion, neofunctionalizing and pseudogenizing over time. Olfaction is a primary sense used for food detection in plant-visiting bats, but the relationship between dietary specialization and OR repertoire diversity is unclear. Within neotropical Leaf-nosed bats (Phyllostomidae), many lineages are plant specialists, and some have a distinct OR repertoire compared to insectivorous species. Yet, whether specialization on particular plant genera is associated with the evolution of specialized, less diverse OR repertoires has never been tested. Using targeted sequence capture, we sequenced the OR repertoires of three sympatric species of short-tailed fruit bats (Carollia), which vary in their degree of specialization on the fruits of Piper plants. We characterized orthologous vs duplicated receptors among Carollia species, and explored the diversity and redundancy of the receptor gene repertoire. At the species level, the most dedicated Piper specialist, Carollia castanea, had lower OR diversity compared to the two generalists (C. sowelli and C. perspicillata), but we discovered a few unique sets of ORs within C. castanea with high redundancy of similar gene duplicates. These unique receptors potentially enable C. castanea to detect Piper fruit odorants better than its two congeners. Carollia perspicillata, the species with the most generalist diet, had a higher diversity of intact receptors, suggesting the ability to detect a wider range of odorant molecules. Variation among ORs may be a factor in the coexistence of these sympatric species, facilitating the exploitation of different plant resources. Our study sheds light on how gene duplication and changes in OR diversity may play a role in dietary adaptations and underlie ecological interactions between bats and plants.
Loss of muscleblind splicing factor shortens Caenorhabditis elegans lifespan by reducing the activity of p38 MAPK/PMK-1 and transcription factors ATF-7 and Nrf/SKN-1
Authors: Matilainen Olli , Ribeiro Ana R S , Verbeeren Jens , Cetinbas Murat , Sood Heini , Sadreyev Ruslan I , Garcia Susana M D A 
Published: July 2021

Abstract
Muscleblind-like splicing regulators (MBNLs) are RNA-binding factors that have an important role in developmental processes. Dysfunction of these factors is a key contributor of different neuromuscular degenerative disorders, including Myotonic Dystrophy type 1 (DM1). Since DM1 is a multisystemic disease characterized by symptoms resembling accelerated aging, we asked which cellular processes do MBNLs regulate that make them necessary for normal lifespan. By utilizing the model organism Caenorhabditis elegans, we found that loss of MBL-1 (the sole ortholog of mammalian MBNLs), which is known to be required for normal lifespan, shortens lifespan by decreasing the activity of p38 MAPK/PMK-1 as well as the function of transcription factors ATF-7 and SKN-1. Furthermore, we show that mitochondrial stress caused by the knockdown of mitochondrial electron transport chain components promotes the longevity of mbl-1 mutants in a partially PMK-1-dependent manner. Together, the data establish a mechanism of how DM1-associated loss of muscleblind affects lifespan. Furthermore, this study suggests that mitochondrial stress could alleviate symptoms caused by the dysfunction of muscleblind splicing factor, creating a potential approach to investigate for therapy.
ANNORE: genetic fine-mapping with functional annotation
Authors: Fisher Virginia , Sebastiani Paola , Cupples L Adrienne , Liu Ching-Ti 
Published: July 2021

Abstract
Genome-wide association studies (GWASs) have successfully identified loci of the human genome implicated in numerous complex traits. However, the limitations of this study design make it difficult to identify specific causal variants or biological mechanisms of association. We propose a novel method, AnnoRE, which uses GWAS summary statistics, local correlation structure among genotypes and functional annotation from external databases to prioritize the most plausible causal single-nucleotide polymorphisms (SNPs) in each trait-associated locus. Our proposed method improves upon previous fine-mapping approaches by estimating the effects of functional annotation from genome-wide summary statistics, allowing for the inclusion of many annotation categories. By implementing a multiple regression model with differential shrinkage via random effects, we avoid reductive assumptions on the number of causal SNPs per locus. Application of this method to a large GWAS meta-analysis of body mass index identified six loci with significant evidence in favor of one or more variants. In an additional 24 loci, one or two variants were strongly prioritized over others in the region. The use of functional annotation in genetic fine-mapping studies helps to distinguish between variants in high LD and to identify promising targets for follow-up studies.
Linking genome variants to disease: scalable approaches to test the functional impact of human mutations
Authors: Findlay Gregory M 
Published: July 2021

Abstract
The application of genomics to medicine has accelerated the discovery of mutations underlying disease and has enhanced our knowledge of the molecular underpinnings of diverse pathologies. As the amount of human genetic material queried via sequencing has grown exponentially in recent years, so too has the number of rare variants observed. Despite progress, our ability to distinguish which rare variants have clinical significance remains limited. Over the last decade, however, powerful experimental approaches have emerged to characterize variant effects orders of magnitude faster than before. Fueled by improved DNA synthesis and sequencing and, more recently, by CRISPR/Cas9 genome editing, multiplex functional assays provide a means of generating variant effect data in wide-ranging experimental systems. Here, I review recent applications of multiplex assays that link human variants to disease phenotypes and I describe emerging strategies that will enhance their clinical utility in coming years.
‘To Destroy’: Anna Freud and Dorothy Burlingham in the Freud Archive
Authors: Meadows Agnes 
Published: July 2021

Abstract
In a 1960 letter to her lifelong collaborator Dorothy Burlingham, Anna Freud described the ambiguities of existing as a woman and as a member of the Freud family within psychoanalytic institutions. Burlingham later marked this letter with the words ‘To destroy’ on the grounds that it was ‘too personal’. This article draws on the newly catalogued Dorothy Burlingham collection in the Freud Museum archive to consider both Anna Freud’s letter and Burlingham’s attempt to remove it. One context is a debate around the nature and function of the archive, fought out in letters between Anna Freud, Kurt Eissler and Dorothy Burlingham from 1960 to 1972. In that correspondence Eissler pressured Burlingham to convince Anna Freud to include her manuscripts in the archive. The letters held there also raise the question of Burlingham’s own absence from psychoanalytic histories.
Advanced Regenerative Medicine Strategies for Treatment of Perianal Fistula in Crohn’s Disease
Authors: Nazari Hojjatollah , Naei Vahid Yaghoubi , Tabasi Asieh Heirani , Badripour Abolfazl , Akbari Asbagh Reza , Keramati Mohammad Reza , Sharifi Amirsina , Behboudi Behnam , Kazemeini Alireza , Abbasi Maryam , Keshvari Amir , Ahmadi Tafti Seyed Mohsen 
Published: July 2021

Abstract
Regenerative medicine is an emerging therapeutic method that aims to reconstruct tissues and organs. This advanced therapeutic approach has demonstrated great potential in addressing the limitations of medical and surgical procedures for treating perineal fistula in patients with Crohn’s disease. Recent developments in stem cell technology have led to a massive good manufacturing practices (GMPs) production of various stem cells, including mesenchymal and embryonic cells, along with induction of pluripotent stem cells to repair damaged tissues in the fistula. The recent advances in separation and purification of exosomes, as biologic nanovesicles carrying anti-inflammatory and regenerative agents, have made them powerful tools to treat this inflammatory disease. Further, tremendous advances in nanotechnology, biomaterials, and scaffold fabrication methods enable tissue engineering methods to synthesize tissue-like structures to assist surgical techniques. This review focuses on advanced regenerative-based methods including stem cell therapy, exosome therapy, and tissue engineering used in the treatment of perianal fistula. Relevant in vitro and in vivo studies and the latest innovations in implementation of regenerative medicine for this disease are also separately reviewed. Additionally, current challenges regarding implementation of g stem cells, exosomes, and tissue engineering methods for bridging the gaps between laboratory findings and clinic application will be discussed.
Outsourcing Hotspot Governance within the EU: Cultural Mediators as Humanitarian–Border Workers in Greece
Authors: Spathopoulou Aila , Pauliina Kallio Kirsi , Hakli Jouni 
Published: July 2021

Abstract
Responding to the self-declared “Mediterranean migration crisis” in 2015, the European Commission launched a Hotspot Approach to speed up the handling of incoming migrants in the “frontline states” of Greece and Italy. A key element in this operation is the identification of those eligible for asylum, which requires effective communication across cultural and linguistic difference between the asylum system and the migrants, facilitated by officially designated “cultural mediators.” We assess the hotspot governance as a form of outsourcing border control within the EU territory. Beyond sorting out and separating migrants into the categories of deservingness and undeservingness, we propose that the hotspot mechanism represents “governing by communication,” with cultural mediators as key players in this humanitarian–bordering strategy. A focus on how cultural mediators provide the precarious human labor for this governance, offers, we argue, a productive inroad into the ways in which the hotspot economies of deterrence, containment, and care sustain inequalities embedded in race, socioeconomic status, and citizenship.
A meta-analytical evaluation of the effects of high-salt water intake on beef cattle
Authors: López Agustín , Arroquy Jose I , Hernández Olegario , Nasca Jose A , Juárez Sequeira Ana V , DiLorenzo Nicolas , Distel Roberto A 
Published: July 2021

AbstractAdequate drinking water is essential to maintain acceptable production levels in beef cattle operations. In the context of global climate change, the water scarcity forecasted for the future is a growing concern and it would determine an increase in the use of poorer quality water by the agricultural sector in many parts of the world. However, consumption of high-salt water by cattle has consequences often overlooked. A meta-analysis was carried out to assess the impact of utilizing high-salt water on dry matter (DMI) and water intake (WI), and performance in beef cattle. The dataset was collected from 25 studies, which were conducted between 1960 and 2020. Within the dataset, the water quality was divided into three categories according to the ratio of sulfates (SO4) or sodium chloride (NaCl) to total dissolved solids (TDS): 1) TDS = all studies included (average SO4:TDS = 0.4); 2) NaCl = considered studies in which water salinity was dominated by NaCl (average SO4:TDS = 0.1); and 3) SO4 = considered studies in which water salinity was dominated by SO4 (average SO4:TDS = 0.8). Results showed that DMI and WI were negatively affected by high-salt water consumption, although the magnitude of the effect is dependent on the type of salt dissolved in the water. There was a quadratic effect (P &lt; 0.01) for the WI vs. TDS, WI vs. NaCl, DMI vs. TDS, and DMI vs. NaCl, and a linear effect (P &lt; 0.01) for WI vs. SO4 and WI vs. SO4. Average daily gain (ADG) and feed efficiency (FE) were quadratically (P &lt; 0.01) affected by high-salt water, respectively. This study revealed significant negative effects of high-salt water drinking on beef cattle WI, DMI, and performance. However, the negative effects are exacerbated when cattle drink high-sulfate water when compared with high-chloride water. To the best of our knowledge, this is the first approach to evaluate animal response to high-salt water consumption and could be included in the development of future beef cattle models to account for the impact of water quality on intake and performance. In addition, this meta-analysis highlights the need for research on management strategies to mitigate the negative effects of high-salt water in cattle.
Policing the Digital Divide: Institutional Gate-keeping & Criminalizing Digital Inclusion
Authors: Yang Tian , Ticona Julia , Lelkes Yphtach 
Published: July 2021

AbstractPrevious studies generally assume that barriers to internet access are largely passive. That is, exclusion from the Internet is a consequence of poorly resourced individuals, communities, and institutions. This study complicates that assumption by focusing on the active policing and gatekeeping of internet access. Specifically, we estimate the causal effect of free Wi-Fi at chain restaurants on quality-of-life crime reporting by leveraging a staggered difference-in-differences design which compares geo-located crime reports near chain restaurants in Chicago before and after those restaurants introduced free Wi-Fi. We find that free Wi-Fi led to a substantive and significant increase in quality-of-life policing when restaurants were located in wealthier and Whiter areas, but not in other areas. Our findings suggest that internet access itself may be actively policed by social institutions, in our case, national chain restaurants and the police, to protect access for some at the expense of others.
Forester Preferences on RevisingSilvics of North America: A Survey of Registered Foresters in Four States
Authors: Merry Krista , Bettinger Pete , Siry Jacek , McNulty Steve , Gavazzi Michael 
Published: July 2021

AbstractSilvics of North America (SNA) is a widely used silvicultural manual. However, the last SNA was published in 1990. Therefore, 2,589 registered foresters were surveyed across four US states to answer two broad questions: (1) Should SNA be updated? And (2) would an updated SNA still be a useful tool for forest management? Most respondents indicated that the type and extent of content contained in the previous version of SNA was still desirable today. Aside from updating all sections with recent research findings, expansion was suggested for ecosystem services provided by species. An update to maps provided in SNA, through changes to natural ranges of tree species and other aesthetic improvement, was suggested by most survey respondents. These findings support the conclusion that the SNA would still be an essential source of information if 21st-century issues such as climate change impacts and adaptation measures were included in the update.
Investigation of fluid flow and heat transfer characteristics for a thermal anti-icing system of a high-altitude and long-endurance UAV
Authors: Cheng Jen-Chieh , Chen You-Ming 
Published: July 2021

ABSTRACT
This research performs a three-dimensional simulation to investigate the fluid flow and heat transfer characteristics for hot-air jets impinging on the wing leading-edge surface. Both the periodic model and the whole model are proposed to examine the thermal anti-icing performance for hot air ejecting from a piccolo tube onto the impinging surface. The results show that, for the periodic model, the enhancement of the average Nusselt number can be up to 94.4%, and the enhancement of the average heat flux is up to 29.7% for 100 ≦ uj ≦ 350 m/s and 300 ≦ Tj ≦ 550 K when compared with the results of the basic case of uj = 200 m/s and Tj = 450 K. The maximum enhancement of the $\overline {Nu} $ is 62.3% as the spacing decreases from Sn = 8 to Sn = 4 and the optimum Numax and $\overline {Nu} $ occur at Si = 5 and Si = 6 for the single-array holes with 3 ≦ Si ≦ 7 and 4 ≦ Sn ≦ 8. In addition, the θh for maximum $\overline {{{Nu}}} $ is 10° and the maximum enhancement of the $\overline {{{Nu}}} $ is ∼15.7% for double-array holes and staggered-array holes as compared with single-array holes. In addition, the nonuniformity of Nusselt number and heat flux distributions are significantly improved. For the whole model, the maximum enhancement of the average Nusselt number is ∼7.5% and the optimum configuration is θh = 40°, for cases with La = 60, Dp = 8, $\dot{m}$ = 0.15 kg/s, Si = 6, 1 ≦ Nh ≦ 5, 10 ≦ Sn ≦ 30 and 10° ≦ θh ≦ 60°.
Use of Selective Arterial Calcium Stimulation Testing in Identification of Insulinoma in a Patient After Bariatric Surgery
Authors: Szczepanski Julianne M , Hissong Erika , Manthei David M 
Published: July 2021

Abstract

Objective
Insulinomas are rare in the post-bariatric surgery setting. The differential diagnosis for hypoglycemia is broad, requiring laboratory testing to verify endogenous hyperinsulinemic hypoglycemia. Selective arterial calcium stimulation testing (SACST) can help localize abnormal insulin production. We describe a patient with histologically confirmed insulinoma after bariatric surgery diagnosed with the aid of SACST.

Methods
We present a 67 year old woman with a history of Roux-en-Y bypass surgery who presented with endogenous hyperinsulinemic hypoglycemia. Initially, no pancreatic lesion was identified radiologically. We pursued SACST to localize the source of insulin production.

Results
The SACST successfully localized the source of hyperfunctioning islet cells to the pancreatic tail with absolute insulin values in a range consistent with insulinoma. Additional radiologic studies showed a small tumor in the pancreatic tail. Pathology showed a well-differentiated neuroendocrine tumor, compatible with insulinoma.

Conclusion
This case study illustrates the usefulness of SACST for the diagnosis and localization of insulinoma.
Radio signals from early direct collapse black holes
Authors: Yue B , Ferrara A 
Published: July 2021

ABSTRACT
We explore the possibility to detect the continuum radio signal from direct collapse black holes (DCBHs) by upcoming radio telescopes such as the SKA and ngVLA, assuming that after formation they can launch and sustain powerful jets at the accretion stage. We assume that the high-z DCBHs have similar jet properties as the observed radio-loud AGNs, then use a jet model to predict their radio flux detectability. If the jet power Pjet ≳ 1042–1043 erg s−1, it can be detectable by SKA/ngVLA, depending on the jet inclination angle. Considering the relation between jet power and black hole mass and spin, generally, jetted DCBHs with mass $\gtrsim 10^5\, {\rm M}_\odot$ can be detected. For a total jetted DCBH number density of ∼2.5 × 10−3 Mpc−3 at z = 10, about 100 deg−2z−1 DCBHs are expected to be above the detection threshold of SKA1-mid (100-h integration). If the jet ‘blob’ emitting most of the radio signal is dense and highly relativistic, then the DCBH would only feebly emit in the SKA-low band, because of self-synchrotron absorption (SSA) and blueshift. Moreover, the free–free absorption in the DCBH envelope may further reduce the signal in the SKA-low band. Thus, combining SKA-low and SKA-mid observations might provide a potential tool to distinguish a DCBH from a normal star-forming galaxy.
HCN/HNC chemistry in shocks: a study of L1157-B1 with ASAI
Authors: Lefloch B , Busquet G , Viti S , Vastel C , Mendoza E , Benedettini M , Codella C , Podio L , Schutzer A , Rivera-Ortiz P R , Lépine J R D , Bachiller R 
Published: July 2021

ABSTRACT
Hydrogen cyanide (HCN) and its isomer hydrogen isocyanide (HNC) play an important role in molecular cloud chemistry and the formation of more complex molecules. We investigate here the impact of protostellar shocks on the HCN and HNC abundances from high-sensitivity IRAM 30 m observations of the prototypical shock region L1157-B1 and the envelope of the associated Class 0 protostar, as a proxy for the pre-shock gas. The isotopologues H12CN, HN12C, H13CN, HN13C, HC15N, H15NC, DCN, and DNC were all detected towards both regions. Abundances and excitation conditions were obtained from radiative transfer analysis of molecular line emission under the assumption of local thermodynamical equilibrium. In the pre-shock gas, the abundances of the HCN and HNC isotopologues are similar to those encountered in dark clouds, with an HCN/HNC abundance ratio ≈1 for all isotopologues. A strong D-enrichment (D/H ≈ 0.06) is measured in the pre-shock gas. There is no evidence of 15N fractionation neither in the quiescent nor in the shocked gas. At the passage of the shock, the HCN and HNC abundances increase in the gas phase in different manners so that the HCN/HNC relative abundance ratio increases by a factor 20. The gas-grain chemical and shock model uclchem allows us to reproduce the observed trends for a C-type shock with pre-shock density n(H) = $10^5\hbox{cm$^{-3}$}$ and shock velocity $V_\mathrm{ s}= 40\hbox{kms$^{-1}$}$. We conclude that the HCN/HNC variations across the shock are mainly caused by the sputtering of the grain mantle material in relation with the history of the grain ices.
The radial acceleration relation in a ΛCDM universe
Authors: Paranjape Aseem , Sheth Ravi K 
Published: July 2021

ABSTRACT
We study the radial acceleration relation (RAR) between the total (atot) and baryonic (abary) centripetal acceleration profiles of central galaxies in the cold dark matter (CDM) paradigm. We analytically show that the RAR is intimately connected with the physics of the quasi-adiabatic relaxation of dark matter in the presence of baryons in deep potential wells. This cleanly demonstrates how the mean RAR and its scatter emerge in the low-acceleration regime ($10^{-12}{\rm \, m\, s}^{-2}\lesssim a_{\rm bary}\lesssim 10^{-10}{\rm \, m\, s}^{-2}$) from an interplay between baryonic feedback processes and the distribution of CDM in dark haloes. Our framework allows us to go further and study both higher and lower accelerations in detail, using analytical approximations and a realistic mock catalogue of ${\sim}342\, 000$ low-redshift central galaxies with Mr ≤ −19. We show that, while the RAR in the baryon-dominated high-acceleration regime ($a_{\rm bary}\gtrsim 10^{-10}{\rm \, m\, s}^{-2}$) is very sensitive to details of the relaxation physics, a simple ‘baryonification’ prescription matching the relaxation results of hydrodynamical CDM simulations is remarkably successful in reproducing the observed RAR without any tuning. And in the (currently unobserved) ultra-low-acceleration regime ($a_{\rm bary}\lesssim 10^{-12}{\rm \, m\, s}^{-2}$), the RAR is sensitive to the abundance of diffuse gas in the halo outskirts, with our default model predicting a distinctive break from a simple power-law-like relation for H i-deficient, diffuse gas-rich centrals. Our mocks also show that the RAR provides more robust, testable predictions of the ΛCDM paradigm at galactic scales, with implications for alternative gravity theories than the baryonic Tully–Fisher relation.