bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary breast CD20-positive extranodal NK/T cell lymphoma with stomach involvement: a case report and literature review
Authors: Zhang Ying , Wang Kuansong , Tan Qian , Yang Keda , Wu Dengshu , Xu Yajing , Zhao Xielan , Jiang Zhiping 
Published: November 2021

Abstract
Background
We present a unique case of primary breast CD20-positive extranodal NK/T cell lymphoma with stomach involvement in a young Chinese female patient.

Case presentation
The patient presented with a mass in her right breast that rapidly increased in size over approximately 2 months. Upper gastrointestinal endoscopy showed a giant serpentine ulcer in the stomach. Biopsy was performed, and microscopic inspection revealed that the fibrous tissue was diffusely involved by medium to large abnormal lymphocytes. The cytoplasm was low to moderate. The tumor cells had irregular nuclei and inconspicuous nucleoli. The lymphoid cells were strongly immunoreactive to CD20, CD3, CD4, CD56, TIA-1, EBER, and Ki-67 (90%). Epstein-Barr virus genomes were also found in tumor cells by in situ hybridization. A whole-body positron emission tomography (PET)-CT scan revealed intense FDG uptake in the right breast and greater curvature of the stomach. Monoclonal rearrangements of the T cell receptor (TCR-γ) and immunoglobulin heavy chain (IgH) were identified by genetic analysis. Whole-genome next-generation sequencing was performed, and up to 12 gene mutations, including a frameshift mutation in exon 4 of the BCOR (G97Rfs*87; 44.3%) gene and a base substitution mutation (Q61H 17.6%) in exon 3 of the KRAS gene, were detected. Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed using the database for annotation, visualization, and integrated discovery, which showed that rare primary breast CD20-positive extranodal NK/T cell lymphoma had a unique genetic background compared with diffuse large B cell lymphoma and extranodal NK/T cell lymphoma without CD20 expression. The patient received four cycles of the modified SMILE regimen. The second whole-body PET-CT scan revealed that the right breast mass was significantly smaller than before; additionally, FDG uptake in the stomach wall disappeared.

Conclusions
Systemic examination, extensive immunohistochemistry, and molecular profiling are essential for an accurate diagnosis. More similar cases are required to clarify the biological pathways and even the potential molecular mechanisms of rare lymphomas, which may help direct further treatment.
Impact of low-dose calcipotriol ointment on wound healing, pruritus and pain in patients with dystrophic epidermolysis bullosa: A randomized, double-blind, placebo-controlled trial
Authors: Guttmann-Gruber Christina , Piñón Hofbauer Josefina , Tockner Birgit , Reichl Victoria , Klausegger Alfred , Hofbauer Peter , Wolkersdorfer Martin , Tham Khek-Chian , Lim Seong Soo , Common John E. , Diem Anja , Ude-Schoder Katharina , Hitzl Wolfgang , Lagler Florian , Reichelt Julia , Bauer Johann W. , Lang Roland , Laimer Martin 
Published: November 2021

Abstract
Background
Wound management is a critical factor when treating patients with the inherited skin fragility disease dystrophic epidermolysis bullosa (DEB). Due to genetic defects in structural proteins, skin and mucous epithelia are prone to blistering and chronic wounding upon minor trauma. Furthermore, these wounds are commonly associated with excessive pruritus and predispose to the development of life-threatening squamous cell carcinomas, underscoring the unmet need for new therapeutic options to improve wound healing in this patient cohort. Vitamin D3 is acknowledged to play an important role in wound healing by modulating different cellular processes that impact epidermal homeostasis and immune responses. In this study, we evaluate the safety and efficacy of low-dose calcipotriol, a vitamin D3 analogue, in promoting wound healing and reducing itch and pain in patients with DEB.

Methods
Eligible DEB patients, aged ≥ 6 years and with a known mutation in the COL7A1 gene, were recruited to a placebo-controlled, randomized, double blind, cross-over phase II monocentric clinical trial. Patients were required to have at least two wounds with a minimum size of 6 cm2 per wound. The primary objective was to evaluate efficacy of daily topical application of a 0.05 µg/g calcipotriol ointment in reducing wound size within a 4-week treatment regimen. Secondary objectives were to assess safety, as well as the impact of treatment on pruritus, pain, and bacterial wound colonization in these patients.

Results
Six patients completed the clinical trial and were included into the final analysis. Topical low-dose calcipotriol treatment led to a significant reduction in wound area at day 14 compared to placebo (88.4% vs. 65.5%, P < 0.05). Patients also reported a significant reduction of pruritus with calcipotriol ointment compared to placebo over the entire course of the treatment as shown by itch scores of 3.16 vs 4.83 (P < 0.05) and 1.83 vs 5.52 (P < 0.0001) at days 14 and 28, respectively. Treatment with low-dose calcipotriol did not affect serum calcium levels and improved the species richness of the wound microbiome, albeit with no statistical significance.

Conclusions
Our results show that topical treatment with low-dose calcipotriol can accelerate wound closure and significantly reduces itch, and can be considered a safe and readily-available option to improve local wound care in DEB patients.
TrialRegistration EudraCT: 2016–001,967-35. Registered 28 June 2016, https://www.clinicaltrialsregister.eu/ctr-search/trial/2016-001967-35/AT
Molecular motor KIF3B in the prelimbic cortex constrains the consolidation of contextual fear memory
Authors: Joseph Nadine F. , Zucca Aya , Wingfield Jenna L. , Espadas Isabel , Page Damon , Puthanveettil Sathyanarayanan V. 
Published: November 2021

AbstractMolecular and cellular mechanisms underlying the role of the prelimbic cortex in contextual fear memory remain elusive. Here we examined the kinesin family of molecular motor proteins (KIFs) in the prelimbic cortex for their role in mediating contextual fear, a form of associative memory. KIFs function as critical mediators of synaptic transmission and plasticity by their ability to modulate microtubule function and transport of gene products. However, the regulation and function of KIFs in the prelimbic cortex insofar as mediating memory consolidation is not known. We find that within one hour of contextual fear conditioning, the expression of KIF3B is upregulated in the prelimbic but not the infralimbic cortex. Importantly, lentiviral-mediated knockdown of KIF3B in the prelimbic cortex produces deficits in consolidation while reducing freezing behavior during extinction of contextual fear. We also find that the depletion of KIF3B increases spine density within prelimbic neurons. Taken together, these results illuminate a key role for KIF3B in the prelimbic cortex as far as mediating contextual fear memory.
Measuring the effects of nurse practitioner (NP)-led care on depression and anxiety levels in people with multiple sclerosis: a study protocol for a randomized controlled trial
Authors: Smyth Penelope , Watson Kaitlyn E. , Tsuyuki Ross T. 
Published: November 2021

Abstract
Background
Canada has one of the highest rates of multiple sclerosis (MS) in the world. Treatments and supports for people with MS (PwMS) have become increasingly complex, requiring individualized and adaptive care. Specialized NPs provide advanced skills to those with complex medical conditions, with potential to enhance the health, functioning, and quality of life for PwMS. This study aims to determine the effect of a Nurse Practitioner (NP) on depression and anxiety levels in PwMS.

Methods
We will perform a parallel randomized controlled trial. PwMS who are followed by general private-practice neurologists will be randomly assigned to the intervention group (NP-led care) or the ‘usual care’ control group (general neurologist or family physician and registered nurse support). In the intervention group, the NP will assess and provide care to the MS patient and their caregiver at a baseline visit, with 3-month and 6-month follow-up visits. PwMS in the control group will receive usual care provided by their community neurologists or family physicians with the standard assistance provided by registered nurses experienced in MS care. The primary outcome will be the difference in change in the patient’s anxiety and depression scores as measured by the validated Hospital Anxiety and Depression Scale (HADS) questionnaire at 3 months. Secondary outcomes will include difference in change in HADS at 6 months; Modified Fatigue Impact Scale scores (MSIF) at 3 and 6 months; EQ-5D scores at 3 and 6 months; caregiver health-related quality of life in MS measures (CAREQOL-MS) at 3 and 6 months; number of visits and phone calls to healthcare professionals recorded by patient, and satisfaction with NP-led care vs usual care measured by the validated Consultant Satisfaction Questionnaire.

Discussion
Findings from this study will contribute to exploring benefits of advanced nursing practitioner interventions for PwMS followed by general neurologists and family physicians in a community setting. It will provide evidence of the benefits of NP-led care for PwMS and offer an alternative healthcare resource for management of MS.

Trial registration
ClinicalTrials.govPro00069595. Retrospectively registered on June 26, 2020. Protocol version: January 2017, version 1.
Definitions and elements of endpoints in phase III randomized trials for the treatment of COVID-19: a cross-sectional analysis of trials registered in ClinicalTrials.gov
Authors: Sakamaki Kentaro , Uemura Yukari , Shimizu Yosuke 
Published: November 2021

Abstract
Background
There are several challenges in designing clinical trials for the treatment of novel infectious diseases, such as COVID-19. In particular, the definition of endpoints related to the severity, time frame, and clinical course remains unclear. Therefore, we conducted a cross-sectional analysis of phase III randomized trials for COVID-19 registered at ClinicalTrials.gov.

Methods
We collected the data from ClinicalTrials.gov on March 31, 2021, by specifying the following search conditions under Advanced Search: Condition or disease: (COVID-19) OR (SARS-CoV-2); Study type: Interventional Studies; Study Results: All Studies; Recruitment: Not yet recruiting, Recruiting, Enrolling by invitation, Active, Not recruiting, Suspended, Completed; Sex: All; and Phase: Phase 3. From the downloaded search results, we selected trials that met the following criteria: Primary Purpose: Treatment; Allocation: Randomized. We manually transcribed information not included in the downloaded file, such as Primary Outcome Measures, Secondary Outcome Measures, Time Frame, and Inclusion Criteria. In the analysis, we examined primary and secondary endpoints in trials with severe and non-severe patients, including the types of endpoints, time frame, clinical course, and sample size.

Results
A total of 406 trials were included in the analysis. The median numbers of endpoints in trials with severe and non-severe patients were 9 and 7, respectively. Approximately 25% of the trials used multiple primary endpoints. Regardless of the type of endpoint, the time frames were longer in the trials with severe patients than in the trials with non-severe patients. In the evaluation of the clinical course, worsening was often considered in binary endpoints, and improvement was considered in time-to-event endpoints. The sample size was the largest in clinical trials using binary endpoints.

Conclusions
Endpoints can differ with respect to severity, and the clinical course and time frame are important for defining endpoints. This study provides information that can facilitate the achievement of a consensus for the endpoints in evaluating COVID-19 treatments.
Integrating hazard, exposure, vulnerability and resilience for risk and emergency management in a volcanic context: the ADVISE model
Authors: Bonadonna Costanza , Frischknecht Corine , Menoni Scira , Romerio Franco , Gregg Chris E. , Rosi Mauro , Biass Sebastien , Asgary Ali , Pistolesi Marco , Guobadia Dehrick , Gattuso Alessandro , Ricciardi Antonio , Cristiani Chiara 
Published: November 2021

AbstractRisk assessments in volcanic contexts are complicated by the multi-hazard nature of both unrest and eruption phases, which frequently occur over a wide range of spatial and temporal scales. As an attempt to capture the multi-dimensional and dynamic nature of volcanic risk, we developed an integrAteD VolcanIc risk asSEssment (ADVISE) model that focuses on two temporal dimensions that authorities have to address in a volcanic context: short-term emergency management and long-term risk management. The output of risk assessment in the ADVISE model is expressed in terms of potential physical, functional, and systemic damage, determined by combining the available information on hazard, exposed systems and vulnerability. The ADVISE model permits qualitative, semi-quantitative and quantitative risk assessment depending on the final objective and on the available information. The proposed approach has evolved over a decade of study on the volcanic island of Vulcano (Italy), where recent signs of unrest combined with uncontrolled urban development and significant seasonal variations of exposed population result in highly dynamic volcanic risk. For the sake of illustration of all the steps of the ADVISE model, we focus here on the risk assessment of the transport system in relation to the tephra fallout associated with a long-lasting Vulcanian cycle.
Dynamics model analysis of bacteriophage infection of bacteria
Authors: Li Xiaoping , Huang Rong , He Minyuan 
Published: November 2021

AbstractA bacteriophage (in short, phage) is a virus that can infect and replicate within bacteria. Assuming that uninfected and infected bacteria are capable of reproducing with logistic law, we investigate a model of bacteriophage infection that resembles simple SI-models widely used in epidemiology. The dynamics of host-parasite co-extinctions may exhibit four scenarios: hosts and parasites go extinct, parasites go extinct, hosts go extinct, and hosts and parasites coexist. By using the Jacobian matrix and Bendixson–Dulac theory, local and global stability analysis of uninfected and infected steady states is provided; the basic reproduction number of the model is given; general results are supported by numerical simulations. We show that bacteriophages can reduce a host density. This provides a theoretical framework for studying the problem of whether phages can effectively prevent, control, and treat infectious diseases.
Measuring mental wellness among adolescents living with a physical chronic condition: a systematic review of the mental health and mental well-being instruments
Authors: Orth Zaida , van Wyk Brian 
Published: November 2021

Abstract
Background
Globally, promoting mental health and well-being among adolescents has become a public health priority, especially for adolescents living with a physical chronic condition (CC), as research suggests they may be more at risk of developing mental health co-morbidities. Valid and reliable instruments are needed to measure and better understand mental health and mental well-being among adolescents living with a CC. To this end, we reviewed studies reporting on mental health and well-being instruments used in adolescent populations living with a chronic physical condition/disease globally.

Methods
We used a systematic review method guided by PRISMA to identify assess mental health and mental well-being instruments used in adolescents living with a CC. In this instance, mental health instruments were defined as those representing negative domains of mental health (i.e. depression and anxiety) while mental well-being instruments included positive aspects of mental health (i.e. self-concept and resilience).

Results
We identified 22 articles, which included 31 instruments that were used to measure either mental health (n = 8) or mental well-being (n = 15) or both (n = 8) in adolescents living with a CC. Of these, thirteen studies used a Health-Related Quality of Life (HRQoL) scale to measure mental health and/or mental well-being. The KIDSCREEN questionnaires and the Strengths and Difficulties Questionnaire were identified as being frequently used across the 22 studies. Additionally, 7 out of the 31 instruments were disease specific, with 3 focusing on adolescents with diabetes. All the instruments were developed in high income countries and adapted for use in lower- and middle-income countries (LMICs). Adolescents with Type 1 Diabetes (n = 7) and HIV (n = 4) were researched in 11 out of 22 studies. Only eight studies were conducted in LMIC, of which four were in Africa.

Conclusions
HRQoL instruments are useful in measuring mental health and well-being in adolescents living with a CC. However, relatively few valid measures of mental health and mental well-being for adolescents living with a CC exist, which accentuates the paucity of research on mental health and mental well-being of adolescents who are living with a CC. Specific measures need to be developed in and for LMICs where cultural contexts affect mental well-being in unique ways.
Systematic review registration: PROSPERO CRD42020186707.
The affectability of writing assessment scores: a G-theory analysis of rater, task, and scoring method contribution
Authors: Khodi Ali 
Published: November 2021

AbstractThe present study attempted to to investigate factors which affect EFL writing scores through using generalizability theory (G-theory). To this purpose, one hundred and twenty students participated in one independent and one integrated writing tasks. Proceeding, their performances were scored by six raters: one self-rating, three peers,-rating and two instructors-rating. The main purpose of the sudy was to determine the relative and absolute contributions of different facets such as student, rater, task, method of scoring, and background of education to the validity of writing assessment scores. The results indicated three major sources of variance: (a) the student by task by method of scoring (nested in background of education) interaction (STM:B) with 31.8% contribution to the total variance, (b) the student by rater by task by method of scoring (nested in background of education) interaction (SRTM:B) with 26.5% of contribution to the total variance, and (c) the student by rater by method of scoring (nested in background of education) interaction (SRM:B) with 17.6% of the contribution. With regard to the G-coefficients in G-study (relative G-coefficient ≥ 0.86), it was also found that the result of the assessment was highly valid and reliable. The sources of error variance were detected as the student by rater (nested in background of education) (SR:B) and rater by background of education with 99.2% and 0.8% contribution to the error variance, respectively. Additionally, ten separate G-studies were conducted to investigate the contribution of different facets across rater, task, and methods of scoring as differentiation facet. These studies suggested that peer rating, analytical scoring method, and integrated writing tasks were the most reliable and generalizable designs of the writing assessments. Finally, five decision-making studies (D-studies) in optimization level were conducted and it was indicated that at least four raters (with G-coefficient = 0.80) are necessary for a valid and reliable assessment. Based on these results, to achieve the greatest gain in generalizability, teachers should have their students take two writing assessments and their performance should be rated on at least two scoring methods by at least four raters.
Three-dimensional assessment of airway volumes in patients with unilateral cleft lip and palate
Authors: Kiaee Bita , Nucci Ludovica , Sarkarat Farzin , Talaeipour Ahmad Reza , Eslami Sara , Amiri Faezeh , Jamilian Abdolreza 
Published: November 2021

Abstract
Background
Considering the adverse consequences of respiratory insufficiency in cleft lip and palate (CLP) patients, this study aimed to assess the pharyngeal airway dimensions in 9–12-year-old patients with unilateral CLP. This historical cohort evaluated the cone-beam computed tomography (CBCT) scans of 30 patients with non-syndromic unilateral CLP between 9 and 12 years and 30 age- and sex-matched non-cleft controls. Three-dimensional (3D) images were reconstructed by the Mimics software, and the nasopharyngeal, oropharyngeal, and total airway volumes, as well as the minimal cross-sectional area of the airway (minAx), and posterior airway length (PAL) were all measured in the sagittal plane. Data were analyzed by the Student’s t test.

Results
The oropharyngeal and the total airway volumes, as well as the minAx and PAL in CLP patients, were significantly smaller than the corresponding values in the control group (P < 0.05). Despite smaller nasopharyngeal airway volume in CLP patients than controls, this difference was not statistically significant (P > 0.05).

Conclusions
Nine- to twelve-year-old non-syndromic unilateral CLP patients have smaller pharyngeal airway dimensions than non-cleft controls, and are therefore at higher risk of respiratory insufficiency.
Five-year outcomes of EVO implantable collamer lens implantation for the correction of high myopia and super high myopia
Authors: Chen Xun , Wang Xuanqi , Xu Yilin , Cheng Mingrui , Han Tian , Niu LingLing , Wang Xiaoying , Zhou Xingtao 
Published: November 2021

Abstract
Background
To evaluate the long-term safety, efficacy, predictability, and stability of implantable collamer lens with a central hole (EVO ICL) implantation for correcting high myopia (HM) and super high myopia (SHM).

Methods
This prospective study evaluated 83 eyes of 46 patients who were divided into groups based on their spherical equivalent refractive error (SE): HM group (− 12 D ≤ SE < − 6 D) and SHM group (SE < − 12 D). They were followed up for 5 years after ICL implantation; assessments of uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), manifest refractive error, axial length, intraocular pressure, corneal endothelial cell density, and vault were conducted, and a questionnaire was administered.

ResuIts
At 5 years postoperatively, the safety indices of the HM and SHM groups were 1.03 ± 0.10 and 1.32 ± 0.39, and the efficacy indices were 0.83 ± 0.25 and 0.86 ± 0.32, respectively. In the HM group, 60.47% and 79.07% of the eyes were within ± 0.50 D and ± 1.00 D of the attempted correction, while it was achieved for 22.50% and 47.50% of the eyes in the SHM group, respectively. The SE of the HM group decreased from  − 9.72 ± 1.41 D preoperatively to 0.04 ± 0.39 D 1 month postoperatively and − 0.67 ± 0.57 D 5 years postoperatively, while in the SHM group, it decreased from − 15.78 ± 3.06 D preoperatively to  − 0.69 ± 0.97 D 1 month postoperatively and − 1.74 ± 1.19 D 5 years postoperatively.

Conclusion
EVO ICL implantation is safe, effective, and predictable for correcting HM and SHM. CDVA improved more after surgery for SHM, but the growth of axial length still needs attention.
The roles of exosomal immune checkpoint proteins in tumors
Authors: Xing Cheng , Li Heng , Li Rui-Juan , Yin Le , Zhang Hui-Fang , Huang Zi-Neng , Cheng Zhao , Li Ji , Wang Zhi-Hua , Peng Hong-Ling 
Published: November 2021

AbstractTargeting immune checkpoints has achieved great therapeutic effects in the treatment of early-stage tumors. However, most patients develop adaptive resistance to this therapy. The latest evidence demonstrates that tumor-derived exosomes may play a key role in systemic immune suppression and tumor progression. In this article, we highlight the role of exosomal immune checkpoint proteins in tumor immunity, with an emphasis on programmed death ligand 1 (PD-L1) and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), as well as emerging evidence on roles of T cell immunoglobulin-3 (TIM-3), arginase 1 (ARG1), and estrogen receptor binding fragment-associated antigen 9 (EBAG9) expressed by exosomes.