bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Disordered Gut Microbiota on Serum Metabolome Alterations in Colorectal Tumor-Bearing Mice Under The Intervention of Fufangchangtai
Authors: Cai Mengmeng , Lin Zhibing , Xiao Ya , Lu Jinmiao , Wang Xiaoyu , Zhu Shilan , Chen Xiaoyu , Gu Jialin , Ma Yuzhu , Chen Zhaoguo , Huo Jiege 
Published: April 2021

Abstract
Background: The occurrence and development of colorectal cancer is related to the compositional and functional variation of gut microbiota. Compared with healthy people, gut microbiota of patients with colorectal cancer is in disorder. Most traditional Chinese Medicine is effective by oral administration and has both anti-tumor effect and enteric microecological regulative effect. However, whether the dysbiosis of gut microbiota under tumor burden affects the serum metabolome of human body that related to traditional Chinese medicine is unclear. In this study, Fufangchangtai (FFCT) was chosen to be the model prescription to explore the correlation between gut microbiota and the serum metabolism related to FFCT in anti colorectal cancer treatment. Results: The gut microbiota between colorectal tumor-bearing mice and healthy mice were determined by 16S rRNA gene sequencing, showing quite differences between the two groups and suggesting that Firmicutes , Deferribacteres , Bacteroidetes and Proteobacteria were marked differential intestinal bacteria. The alternations in serum metabolome in the FFCT-treating tumor-bearing mice and simple FFCT-treating mice were detected using Liquid chromatogragh-mass spectrometer (LC/MS), showing significant differences between the two groups as well. Metabolites of FFCT like Citric acid, （±）12-HEPE, Cycloartanyl ferulate were much more in simple FFCT-treating mice, indicating that the present of tumor could affect the absorption and metabolism of FFCT. Additionally, these differential metabolites of FFCT involved in multiple pathways including the Alanine, aspartate and glutamate metabolism, Central carbon metabolism in cancer, Biosynthesis of amino acids. Different doses of FFCT were given to the tumor-bearing mice through oral administration, and the results of gut microbiota 16S rRNA gene sequencing showing that FFCT-treating groups has higher abundance of Firmicutes , Turicibacter and Roseburia than tumor-bearing group, moreover, the abundance of these bacteria was positively correlated to the drug concentration. Firmicutes and Bacteroidetes in FFCT-treating groups showed a similar trend with Healthy group, indicating the modulation of FFCT on gut microbiota of colorectal tumor-bearing mice.Conclusions: Collectively, we concluded that the dysbiosis of gut microbiota in tumor-bearing mice could affect the serum metabolome of human body that related to FFCT, and FFCT could correct the gut microbiota of colorectal tumor-bearing mice. It was pointed out that the GM should be concerned during the therapy of FFCT. The more healthier intestinal microenvironment was conductive to the better clinical curative effect.
COVID-19 vaccine acceptance, barriers and facilitators among healthcare workers in Pakistan
Authors: Rehman Khalid , Hakim Muhammad , Arif Nauman , Islam Siraj Ul , Saboor Abdul , Asif Muhammad , Ramharter Michael 
Published: April 2021

Abstract
BackgroundVaccines are one of the main cornerstones in the global initiatives to control the COVID-19 pandemic. National vaccination programs largely depend on vaccine availability and acceptance by a large proportion of the population to confer a measurable impact on SARS-CoV-2 transmission. In this study we determine the acceptance, barriers and facilitators of COVID-19 vaccination amongst healthcare workers (HCWs) in PakistanMethodsA structured and validated questionnaire was used in an online cross-sectional study. Health care workers residing in Pakistan were invited between 31st January to 9th February to participate in this survey ResultsA total of 436 health-care workers took part in this survey and among those 327 (75%) were doctors. 260 (60%) health-care workers indicated that they would accept vaccination against COVID-19. 308 (71%) were completely confident or confident in using USA, UK manufactured COVID-19 vaccine. The acceptance concerning a potential Chinese vaccine was 267 (61%). 292 (67%) strongly agreed or agreed that they are concerned about the efficacy of COVID-19 vaccination while the concerns for safety were 293 (67%) ConclusionsAbout a third of HCWs, a group generally biased towards early acceptance of health interventions – expressed concerns or refusal of vaccination against COVID-19 in this survey. A dedicated communication plan and information campaign addressing the issues of efficacy, quality, logistics and religious concerns is needed to address these hurdles to allow for a successful national vaccination campaign.
Mapping and Identification of Genetic Loci Affecting Earliness of Bolting and Flowering in Lettuce
Authors: Rosental Leah , Still David W. , You Youngsook , Hayes Ryan J. , Simko Ivan 
Published: April 2021

Abstract
Bolting, defined as stem elongation, marks the plant life cycle transition from vegetative to reproductive stage. Lettuce is grown for its leaf rosettes, and premature bolting may reduce crop quality resulting in economic losses. The transition to reproductive stage is a complex process that involves many genetic and environmental factors. In this study the effects of photoperiod and ambient temperature on bolting and flowering regulation was studied by utilizing a lettuce mapping population to identify quantitative trait loci (QTL) and by gene expression analyses of genotypes with contrasting phenotypes. A recombinant inbred line (RIL) population, derived from a cross between PI251246 (early bolting) and cv. Salinas (late bolting), was grown in four combinations of short (8 hours) and long (16 hours) days and low (20°C) and high (35°C) temperature. QTL models revealed both genetic (G) and environmental (E) effects, and GxE interactions. A major QTL for bolting and flowering time was found on chromosome 7 (qFLT7.2) and two candidate genes were identified by fine mapping, homology and gene expression studies. In short days and high temperature conditions qFLT7.2 had no effect on plant development, while several small-effect loci on chromosomes 2, 3, 6, 8 and 9 were associated with bolting and flowering. Of these, the QTL on chromosome 2, qBFr2.1, co-located with the Flowering Locus T (LsFT) gene. Polymorphisms between parent genotypes in the promotor region may explain identified gene expression differences, and were used to design a genetic marker which may be used to identify the late bolting trait.
Internet Addiction and its Associated Factors Among Dilla University Undergraduate Students, Dilla, Ethiopia, 2019: A Cross-Sectional Study
Authors: Mengistu Nebiyu , Tarekegn Desalegn , Molla Wondwosen , Shumye Seid 
Published: April 2021

Abstract
Background: Internet addiction is characterized by excessive or poorly controlled preoccupation, urges or behavior regarding computer use and internet access that lead to impairment or distress. Globally, it has been found that the occurrence rate of internet addiction among university students ranges from 0.8% to 47.7%. Despite, there are multiple challenges that related with internet addiction which remains under-recognized and largely ignored by stakeholders and also not well known especially in low income countries including Ethiopia. Therefore, this study was conducted aimed to assess the magnitude of the internet addiction and its associated factors among undergraduate students. Methods: Institutional based cross-sectional study was employed from May 1st to June 1st 2019. Multi-stage sampling technique was used to get a total of 846 undergraduate Students. Data was collected by using self-administered structured questionnaires of Young’s Internet Addiction Test. The collected data was coded and entered into EPIDATA 3.1 and analyzed by using SPSS version 22, Bivariate and multivariate logistic regression analysis was conducted to identify factors associated with internet addiction and statistically significant was considered at P-value <0.05. Results: A total of 846 study participants, giving a response rate were 761 (90%) and the prevalence of internet addiction was 19.4%. Multiple logistic regression model revealed that being male [AOR=1.69, 95% CI: (1.80, 6.41)], probable depression [AOR =3.61, 95% CI (2.40, 5.43)] and khat or caffeinated drinks [AOR=1.86, 95%CI: (1.21, 2.87)] were significantly associated with internet addiction.Conclusion: This study revealed that a high prevalence of internet addiction among Dilla university students and there were various factors associated with increased prevalence of problematic internet use. Therefore, students need to be educated about safe, valuable, and healthy practice of internet use and better to counsel on substance use and its consequences and also psychiatric evaluation (referring for mental health services) to overcome the anticipated increase in internet addiction
Clinical Analysis of Gut Barrier Dysfunction in Patients with Advanced Colorectal Cancer Undergoing Cytoreductive Surgery and HIPEC
Authors: Ping Le lai , Mian Jiang xu , Wei Chen 
Published: April 2021

Abstract
Introduction: Hyperthermic intraperitoneal chemotherapy combinedwith cytoreductive surgery is a preferred treatment option for advanced colorectal cancer patients. However, little is known whether the HIPEC can cause the damage of gut barrier function.Methods: A total of 123 patients underwent surgical resection for advanced CRC. Sixty-five patients were treated HIPEC after cytoreductive surgery whereas 58 patients underwent surgery only. Gut barrier function were evaluated using the expression of serum DAO/D-la/ET on D1/D5/D10 after surgery. Both groups were compared for patient characteristics, perioperative data and gut barrier function. Moreover, rats received intraperitoneal injection of retetrexed to observe possible changes of colonic structure under optical microscope.Results: Both groups were comparable with respect to general patient characteristics and post-operative complications. The HIPEC+CRS group was associated with a higher postoperative serum level of DAO/D-la on D1/D5 (p < 0.05) and ET on D5 after surgery (p < 0.05) than that of the surgery only group. Ten days after surgery showed no statistical difference between the 2 groups (p > 0.05).A large area structure disorder, epithelial necrosis, glandular deformation and a large number of lymphocytes infiltration was found in the lamina propria in animals received intraperitoneal injection of retetrexed.Conclusion: In this study, CRS combined with HIPEC does have but only an irreversible impact on gut barrier for advanced CRC patients.
Acute Organ Failure and Risk of Admission to Intensive Medical Care in Cancer Patients, A Single Centre Inception Cohort Study
Authors: Coelho Sara , Ribeiro Teresa , Pereira Isabel , Duarte Delfim , Afonso Ana , Meneses Iolanda , Pinelas Sofia , Pereira Brigitte , Coelho Fernando , Martins Anabela , Sousa Nuno , Faria Filomena 
Published: April 2021

Abstract
Background: Risk of acute organ failure (AOF) in cancer patients on systemic antineoplastic treatment is unknown. However, up to 5% of non-hematologic and 15% of hematologic cancer patients will need to be admitted to an intensive care unit (ICU). IPOPSCI-2017/01 is a prospective cohort study designed to ascertain the cumulative incidence of AOF and ICU admission in adult cancer patients. Methods: Single centre prospective cohort study with consecutive sampling of adult cancer patients admitted for unscheduled inpatient care while on, or up to 8 weeks after, systemic cancer treatment. Primary endpoints were cumulative risk of developing AOF and of ICU admission. Six months accrual expected an accrual of 400 patients to infer a population risk of ICU admission with a precision error of 2% and type 1 error of 5%. Results: Between August 2018 and February 2019, 10392 patients were on systemic anti-neoplastic treatment, 358 had unscheduled inpatient care and were eligible for inclusion and 285 were included. Mean age was 60.9 years-old, 50.9% were male, 52.3% had adjusted Charlson Comorbidity Index ≥3 and hematologic cancers accounted for 17.9% of patients. The cumulative risk of AOF on hospital admission was 29.5% (95%CI: 26-33) and during hospital stay was 39.6% (95%CI: 35-44). Cumulative risk of ICU admission of the patients with AOF was 15.0% (95%CI: 12-18) and if artificial life support criteria and AOF, cumulative risk of ICU admission was 31.5% (95%CI:CI: 23-40). On admission, 62.1% of patients were considered not eligible for artificial organ replacement therapy (no-AORT) and 34.3% of patients who developed AOF while in-hospital were judged no-AORT. Overall, 17 (15%) patients with AOF were admitted to ICU, 31.5% for AORT. Median follow up was 9.5 months. Inpatient mortality was 17.5%, with ICU mortality rate of 58.8%, with median cohort survival 134 days (95%CI: 106-162). On multivariate analysis, AOF was an independent poor prognostic factor (HR 1.6; 95%CI: 1.2-2.2). Conclusions: Risk of AOF in cancer patients admitted for unscheduled inpatient care while on systemic treatment is 39.6%, and risk of ICU admission is 15.0%. AOF in cancer patients was an independent poor prognostic factor for inpatient hospital stay and 6-months survival.
Minimally-invasive Tubular Surgery Versus Conventional Surgery in the Treatment of Spinal Metastasis: a Retrospective Case-control Study
Authors: Shi Xuedong , Cui Yunpeng , Mi Chuan , Wang Bing , Li Chunwei , Pan Yuanxing , Lin Yunfei 
Published: April 2021

Abstract
Background: This study aims to evaluate the perioperative safety and efficacy of minimally-invasive tubular surgery for spinal metastasis with different blood supply.Methods: 72 patients with spinal metastasis between January 2011 to June 2020 were retrospectively reviewed. 14 patients underwent minimally-invasive tubular surgery (Mini-invasive group), and 58 patients underwent conventional surgery (Conventional group). T-test and Mann–Whitney U test was used to evaluate the difference in demographic and perioperative data between the two groups.Results: Baseline characteristics did not differ significantly between the two groups except for the Tokuhashi score (p=0.036). Overall, conventional group had significantly more blood loss (P=0.001), blood transfused(P=0.027), drainage(P<0.001), and longer time of drainage tube(P<0.001), postoperative hospitalization(P=0.002) compared with the mini-invasive group. Sub-analysis showed that for patients with hypo-vascular tumor, trans-channel decompression surgery had all advantages mentioned above. For patients with hyper-vascular tumor, trans-channel decompression surgery only had advantages on the drainage related events. Patients with hyper-vascular tumor had significantly more blood loss compared with patients with hypo-vascular tumor among mini-invasive group.Conclusion: In selected cases with spinal metastasis, minimally-invasive tubular surgery is safe and effective for patients with spinal metastasis. Patients with hypo-vascular tumor were more suitable for this technique with less blood loss, less blood transfused, less drainage and shorter postoperative hospitalization.
The Invasive Seaweed Asparagopsis Taxiformis Erodes the Primary Productivity and Biodiversity of Native Algal Forests in the Mediterranean Sea
Authors: Mancusoa F. Paolo , D’Agostaro Riccardo , Milazzo Marco , Badalamenti Fabio , Musco Luigi , Mikac Barbara , Brutto Sabrina Lo , Chemello Renato 
Published: April 2021

Abstract
Invasive seaweeds are listed among the most relevant threats to marine ecosystems worldwide. Biodiversity hotspots, such as the Mediterranean Sea, are facing multiple invasions and are expected to be severely affected by the introduction of new non-native seaweeds in the near future. In this study, we evaluated the consequences of the shift from the native Ericaria brachycarpa to the invasive Asparagopsis taxiformis habitat at shallow rocky shores of Favignana Island (Egadi Islands, MPA, Sicily, Italy). We compared algal biomass and species composition and structure of the associated epifaunal assemblages in homogenous and mixed stands of E. brachycarpa and A. taxiformis. Results showed that the biomass of primary producers is reduced by 90% in the A. taxiformis invaded habitat compared to the E. brachycarpa native habitat. The structure of the epifaunal assemblages displayed significant variations among homogenous and mixed stands. The abundance, species richness and Shannon-Wiener diversity index of the epifaunal assemblages decreased by 89%, 78% and 40%, respectively from homogenous stands of the native E. brachycarpa to the invasive A. taxiformis. Seaweed biomass was the structural attribute better explaining the variation of epifaunal abundance, species richness and diversity. Overall, our results suggest that the shift from E. brachycarpa to A. taxiformis habitat would drastically erode the biomass of primary producers and the associated biodiversity. We hypothesize that a complete shift from native to invasive seaweeds can ultimately lead to bottom-up effects in rocky shore habitats, with negative consequences for the ecosystem structure, functioning and the services provided.
Paclitaxel and Mortality in Patients With Claudication and De Novo Femoropopliteal Lesions: A Historical Cohort Study
Authors: Gahide Gerald , Phaneuf Samuel C. , Cossette Mathilde , Banine Amine , Budimir Martina , Maghsoudloo Kourosh , Fei Phillip , Dou Bo Yi , Bouthillier Maxime , Alain Charles , Bradette Simon , Noel-Lamy Maxime , Belzile Francois , Bui Bao The , Despatis Marc Antoine , Vendrell Jean Francois 
Published: April 2021

Abstract
Objective: To compare the mortality rates of patients with claudication and de novo femoropopliteal lesions treated with and without paclitaxel coated devices (PCD).Background: A recent meta-analysis, mostly including patients with claudication and de novo femoropopliteal lesions but also with recurrent stenoses and critical limb ischemia, has shown a significant excess mortality in patients treated with PCD.Methods: Comparison of two historical cohorts of patients presenting with claudication and de novo femoropopliteal lesions treated with and without PCD at our institution between 2008 and 2018.Results: After review of 5219 arteriograms in patients presenting with peripheral artery disease, 700 consecutive patients were included consisting in 72.6% of male (n=508). Mean age was 68.1±8.5 years. 45.7% of the patients (n=320) had a treatment including a PCD. Mean femoropopliteal lesion length was 123±91mm including 44.6% of occlusions. Patients of the control group were censored at crossover to paclitaxel when applicable. Mortality rates at 1, 2 and 5 years were 4.6%, 7.5%, 19.4% and 1.6%, 6.2%, 16.6% in the non-PCD and PCD groups respectively. The relative risks of death when using PCD were 0.35 (p=0.03), 0.83 (p= NS) and 0.86 (p= NS) at 1, 2 and 5 years respectively. Conclusion: There was no excess mortality in patients with claudication and de novo femoropopliteal lesions treated with paclitaxel coated devices at 1, 2 and 5 years of follow-up. The current study suggests that additional prospective randomized studies properly powered to study mortality are necessary.
Optic Nerve Head Elasticity and Corneal Biomechanics in Patients with Optical Disc Drusen and Optic Disc Edema
Authors: SARAC Ozge , TASCI Yelda Y , YESILIRMAK Nilufer , UNAL Ozlem , ONER Semih , CAGIL Nurullah 
Published: April 2021

Abstract
Purpose: The purpose of the study is to evaluate the efficacy of ultrasound elastography (USE) in differentiating optic disc drusen (ODD) from optic disc edema (ODE) and to investigate the relationship between corneal biomechanics and optic nerve elasticity. Methods: This prospective, clinical study included 28 eyes of 16 ODD patients (Group 1), 23 eyes of 18 ODE patients (Group 2) and 30 eyes of 15 healthy controls (Group 3). Best corrected distance visual acuity (BDVA), corneal hysteresis (CH), corneal resistance factor (CRF), corneal-compensated intraocular pressure (IOPcc), Goldmann-correlated IOP (IOPg) and optic nerve head (ONH)-elasticity were measured. Results: The mean BDVA value was significantly lower in Group 2 compared to Groups 1 and 3 (p<0.001), there was no significant difference between Groups 1 and 3 (p=0.089). The mean intraconal fat to ONH ratio was significantly higher in Group 1 compared to Group 2 (p=0.008) and 3 (p=0.002), there was no significant difference between Group 2 and 3 (p=0.182). The receiver operating characteristic curve areas for ONH-elasticity in differentiating group 1 and group 2 was 0.728 (p=0.008) with 80% sensitivity and 60% specificity when the cut-off point was set at 2.29. Corneal biomechanics (CH, CRF, IOPcc, IOPg) were not different between the three groups (p>0.05), however there was a significant correlation between corneal and ONH biomechanics in drusen group (p<0.05). Conclusion: The evaluation of the ONH with USE seems to provide useful data in differentiating ODD from ODE. Significant correlation was revealed between corneal and ONH biomechanics in drusen group.
A Predictive Model for Hip Abductor Strength and Knee Extensor Strength 12 Months After Total Hip Arthroplasty With an Interaction Term
Authors: Sekita Junya , Takahira Naonobu , Iwamura Genki , Watanabe Hiroyuki , Kusaba Atsushi , Kondo Saiji 
Published: April 2021

Abstract
Background: Identifying populations with poor muscle recovery after total hip arthroplasty (THA) is important for postoperative physical therapy. Preoperative muscle strength is a strong factor that determines postoperative muscle strength. However, this effect may depend on other factors. Thus, predictive models with interaction terms are important for accurately predicting postoperative muscle strength. This study aimed to develop a predictive model for lower muscle strength 12 months after THA which incorporates interaction terms.Methods­: Subjects were female patients with hip osteoarthritis who underwent unilateral THA. Patients with locomotor disorders, neurological disorders, or postoperative complications were excluded. Hip abductor and knee extensor strength were measured, and a generalized linear model approach with preoperative muscle strength, age, body weight, height, disease duration, physical activity, and leg extension as explanatory variables was used to identify factors that determine muscle strength 12 months after THA. Models with interaction terms between preoperative muscle strength and other explanatory variables were also examined. Results: A total of 82 patients were analyzed. Preoperative muscle strength, age, body weight, physical activity, and disease duration were extracted as factors that significantly and independently determine hip abductor and knee extensor strength. The interaction term between preoperative muscle strength and age was identified as a factor that significantly determines knee extensor strength. Regression coefficients for preoperative knee extensor strength and postoperative muscle strength were significant when age was +1 SD, but not when age was -1 SD.Conclusions: The predictive model demonstrated that lower muscle strength 12 months after THA is determined by preoperative muscle strength, age, weight, physical activity, disease duration, and preoperative muscle strength, with the effect of preoperative muscle strength on knee extensor strength being dependent on age. When predicting postoperative knee extensor strength using preoperative muscle strength, it is important to consider the effect of age.