bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spinal Cord Edema in Atypical Cervical Spondylotic Myelopathy--the Facts Behind the Unusual MR Findings: Case Report and Review of the Literature
Authors: Zhu Qingrun , Zhou Xin , Ren Shiwei , Wang Dachuan , Wang Feng 
Published: October 2021

Abstract
Purpose We report the case of a young man with a slow progression of cervical spondyloticmyelopathy (CSM). Cervical magnetic resonance imaging (MRI) revealed a mild cervical discbulgingat C5–C6 and an area of atypically enlarged intramedullary high signal intensity extending from C4–C7 (T2-weighted) with contrast enhancement at C5–C6 (T1-weighted). Therefore, neurologic and radiologic diagnoses favored demyelinating diseases. Interference occurred to the diagnosis of CSM.Methods This was a retrospective case study with follow-up examination and MRI at 3 months, 1 year and 2 years postoperatively.Results Thepatient’s symptoms improved immediately postoperatively. The functional result according to the modified Japanese Orthopedic Association (JOA) score improved from 10 to 13 within 3 months. He continued to improve neurologically over the first postoperative year. Two years postoperatively, a T2-weighted MRI showed that the edema signal had disappeared completely , and a fat-saturated T2-weighted MRI showed only slight abnormal signal. The numbness and weakness of the extremities had improved, and his JOA score was 16. Conclusions Spinal cord edema is occasionally seen with CSM. In the case presented, the contrast enhancement was localized at the site of the greatest narrowing of the spinal canal and compression of the spinal cord. This coincidence was the best indicator of a mechanical cause of the spinal cord changes. This feature should increase physician’s confidence in distinguishing CSM from intramedullary tumors and myelitis. Surgical decompression may be beneficial in improving neurologic outcomes.
Ryanodine Receptor 1-mediated Ca2+ signaling and mitochondrial reprogramming modulate uterine serous cancer malignant phenotypes
Authors: Zhang Li , Huang Chunxian , Yeung Tsz-Lun , Ferri-Borgogno Sammy , AuYeung Chilam , Lawson Barrett C. , Kwan Suet-Ying , Thomas Vienna , Lu Karen H. , Yip Kay-Pong , Sham James S.K. , Mok Samuel 
Published: October 2021

Abstract
Background

Uterine serous cancer (USC) is the most common non-endometrioid subtype of uterine cancer, and is also the most aggressive. Most patients will die of progressively chemotherapy-resistant disease, and the development of new therapies that can target USC remains a major unmet clinical need. This study sought to determine the molecular mechanism by which a novel unfavorable prognostic biomarker RYR1 identified in advanced USC confers their malignant phenotypes, and demonstrated the efficacy of targeting RYR1 by repositioned FDA-approved compounds in USC treatment.
Methods

TCGA USC dataset was analyzed to identify top genes that are associated with patient survival and can be targeted by FDA-approved compounds. The top gene RYR1 was selected and the functional role of RYR1 in USC progression was determined by silencing and over-expressing RYR1 in USC cells in vitro and in vivo. The molecular mechanism and signaling networks associated with the functional role of RYR1 in USC progression were determined by reverse phase protein arrays (RPPA), Western blot, and transcriptomic profiling analyses. The efficacy of the repositioned compound dantrolene on USC progression was determined using both in vitro and in vivo models.
Results

High expression level of ryanodine receptor 1 (RYR1) in the tumors is associated with shortened overall survival. Inhibition of RYR1 suppressed proliferation, migration and enhanced apoptosis through the Ca2+-dependent AKT/CREB/PGC-1α and AKT/HK1/2 signaling pathways, which modulate mitochondrial bioenergetics properties, including oxidative phosphorylation, ATP production, mitochondrial membrane potential, ROS production and TCA metabolites, and glycolytic activities in USC cells. Repositioned compound dantrolene suppressed USC progression in both in vitro and mouse models.
Conclusions

These findings provide insight into the mechanism by which RYR1 modulates the malignant phenotypes of USC and could aid in the development of dantrolene as a repurposed therapeutic agent for the treatment of USC to improve patient survival.
Application of Gaofen-5 Hyperspectral Data in Uranium Exploration: A Case Study of Weijing in Inner Mongolia, China
Authors: Zhang Yuantao , Pan Wei , Yu Zhangfa 
Published: October 2021

Abstract
Gaofen-5 (GF-5) satellite is the world's first full-spectrum hyperspectral satellite to achieve comprehensive observations of the atmosphere and land. The Advanced Hyperspectral Imager (AHSI) carried by GF-5 can acquire 330-chanel imagery covering 390 - 2500 nm. However, the application of GF-5 AHSI imagery in uranium exploration is currently unknown. In this paper, the AHSI imagery was used for prospecting uranium mineralization in the Weijing, Inner Mongolia, China. The matched filter (MF) and threshold segmentation were used for mapping goethite, Al-high, Al-medium and Al-poor sericite. And the principal component analysis (PCA) and gray-level co-occurrence matrix (GLCM) were used to extract the texture information of the study area. Subsequently, combined with geological information, the relationship between alteration information, texture complexity and uranium mineralization was discussed, and it was pointed out that goethite, Al-medium, Al-poor sericite and texture complexity in this area can be used as indicators of uranium mineralization. Finally, three prospects were delineated, which will guide the follow-up uranium exploration in this area and promote the application of GF-5 AHSI data.
Adaptive Contrast Test For Dose-Response Studies
Authors: Kojima Masahiro 
Published: October 2021

Abstract
Background: A confirmation of dose-response is complicated by the need to adjust for multiplicity. We propose a simple and powerful adaptive contrast test with ordinal constraint contrast coefficients determined by observed responses.Methods: The adaptive contrast test can perform using easily calculated contrast coefficients and existing statistical software. We provide the sample SAS program codes of analysis and calculation of power for the adaptive contrast test. After the adaptive contrast test shows the statistically significant dose-response, we consider to select the best dose-response model from multiple dose-response models. Based on the best model, we identify a recommended dose. We demonstrate the adaptive contrast test for sample data. In addition, we show the calculation of coefficient, test statistic, and recommended dose for the actual study. We perform the simulation study with eleven scenarios to evaluate the performance of the adaptive contrast test.Results: We confirmed the statistically significant dose-response for the sample data and the actual study. In the simulation study, we confirmed that the adaptive contrast test has higher power in most scenarios compared to the conventional method. In addition, we confirmed that the type 1 error rate of the adaptive contrast test was maintained at a significance level when there was no difference between the treatment groups.Conclusions: We conclude that the adaptive contrast test can be applied unproblematically to the dose-response study.
Trajectory Tracking For Quadrotors: An Optimization-Based Planning Followed By Controlling Approach
Authors: Kulathunga Geesara , Devitt Dmitry , Klimchik Alexandr 
Published: October 2021

Abstract
We present an optimization-based reference trajectory tracking method for quadrotor robots for slow-speed maneuvers. The proposed method uses planning followed by the controlling paradigm. The basic concept of the proposed method is an analogy to Linear Quadratic Gaussian (LQG) in which Nonlinear Model Predictive Control (NMPC) is employed for predicting optimal control policy in each iteration. Multiple-shooting (MS) is suggested over Direct-collocation (DC) for imposing constraints when modelling the NMPC. Incremental Euclidean Distance Transformation Map (EDTM) is constructed for obtaining the closest free distances relative to the predicted trajectory; these distances are considered obstacle constraints. The reference trajectory is generated, ensuring dynamic feasibility. The objective is to minimize the error between the quadrotor’s current pose and the desired reference trajectory pose in each iteration. Finally, we evaluated the proposed method with two other approaches and showed that our proposal is better than those two in terms of reaching the goal without any collision. Additionally, we published a new dataset, which can be used for evaluating the performance of trajectory tracking algorithms.
Nonlinear Vibration Analysis of Functionally Graded Carbon Nanotube Reinforced Fluid-Conveying Tube in Thermal Environment
Authors: Chen Xu , Zhao Jing-Lei , She Gui-Lin , Jing Yan , Pu Huayan , Luo Jun 
Published: October 2021

Abstract
In this paper, the nonlinear free vibration responses of functionally graded nanocomposite fluid-conveying tube reinforced by single-walled carbon nanotubes (SWNTs) in thermal environment is investigated. The SWCNTs gradient distributed in the thickness direction of the tube forms different reinforcement patterns. The materials properties of the functionally graded carbon nanotube-reinforced composites (FG-CNTRC) are estimated by rule of mixture. A higher-order shear deformation theory and Hamilton’s variational principle are employed to derive the motion equations incorporating the thermal and fluid effects. A two-step perturbation method is implemented to obtain the closed-form asymptotic solution for these nonlinear partial differential equations. The nonlinear frequency under several patterns of reinforcement are presented and discussed. We conducted a series of studies aimed at revealing the effects of the flow velocity, environment temperature, geometrical ratios and carbon nanotube volume fraction on the nature frequency.
The m6A Methyltransferase METTL3 Promotes Cisplatin Resistance and Invasion in Testicular Seminoma via BCL2
Authors: peng jingxuan , Xiang Yali , Lian Wenfei , Chen Hanfei , Peng Dongyi , Yin Yinghao , Chen Qiang , Tang Yuxin 
Published: October 2021

Abstract
Background: Methyltransferase-like 3 (METTL3) involves in promoting tumor progression through tumor-related genes N6-methyladenosine (m6A) modification. Our previous study found that METTL3 plays an important role in seminoma chemosensitivity. BCL-2 is a key cause of cisplatin resistance in many tumors. Therefore, we want to explore whether METTL3 affects cisplatin resistance of seminoma by regulating BCL-2.Methods: In this study, we downregulated and overexpressed METTL3 in TCam-2 cisplatin resistant cells (TCam-2/CDDP). Then, m6A RNA methylation quantification of BCL-2 and cell viability assay, cell apoptosis analysis and cell invasion assay were investigated under the condition of with or without cis‐dichlorodiammine platinum (CDDP) treatment. Results: Consistent with our previous results, METTL3 significantly affects the chemosensitivity of TCam-2/CDDP. After METTL3 downregulated, the proliferation and anti-apoptosis ability of TCam-2/CDDP cells significantly weakened. Correspondingly, overexpression of METTL3 could promote the chemoresistance. However, the phenotype could be partly reversed by decreasing the expression of BCL-2. Moreover, we found that the m6A modification of BCL-2 is more abundant in cisplatin-resistant strains. Knockdown and overexpression of METTL3 significantly affected the m6A modification and the protein level of BCL-2 in TCam-2/CDDP. Finally, we found that METTL3 also promoted the invasion ability of TCam-2/CDDP cells via BCL2.Conclusion: This study revealed that METTL3 promotes anti-apoptosis and invasion of TCam-2/CDDP through BCL-2. And it indicated that METTL3 and BCL-2 may be an effective treatment target for CDDP-resistance seminoma.
The Burden of Coronary Heart Disease and Cancer From Dietary Exposure To Inorganic Arsenic in Adults in China, 2016
Authors: Liu Jialin , Song Wenjing , Li Yiling , Wang Yibaina , Cui Yuan , Huang Jiao , Wang Qi , Wei Sheng 
Published: October 2021

Abstract
Objective: The inorganic arsenic (iAs) could cause a wide range of health damage, including coronary heart disease (CHD) and lung, bladder, and skin cancer. Although dietary iAs intake is the primary source of iAs, the burden of CHD and cancers from dietary iAs exposure in Chinese adults has not been well known. Methods: To estimate the iAs exposure level in Chinese adults’ dietary, we systematically collected food-specific iAs concentrations in China from Chinese and English literature databases during 2000-2020. Food consumption was extracted from two nationwide food and nutrition surveys in China. The population attributable fraction (PAF) was calculated based on the dose-response relationship between iAs and CHD risk. Combining the 2016 Chinese tumor registry data, we calculated the annual incidence of cancer from dietary iAs exposure to measure the disability-adjusted life year (DALY). Results: The total amount of daily foodborne iAs intake was 0.55 μg/kg bw/d among Chinese adults. The DALY of foodborne iAs-associated CHD was 3,017,510 DALYs, which accounted for 10.18% of total CHD DALYs in Chinese adults. Moreover, the carcinogenic DALYs for lung cancer, bladder cancer, and skin cancer of Chinese residents related to dietary iAs were 314.24, 9.89, and 167.32 thousand, accounting for 2.05%, 1.70%, and 35.5% of the respective total cancer burden. Conclusions: Our findings suggested that dietary iAs exposure causes a substantial disease burden in Chinese adults. More efforts for foodborne iAs control are critical to reducing the disease burden of CHD and cancer in China and other countries with similar dietary patterns.
A Pilot Study to Assess the Salivary Gland Regenerative Potential of Bone Marrow Mesenchymal Stromal Cells from Treated Head and Neck Cancer Patients
Authors: Blitzer Grace C , Paz Cristina , Glassey Annemarie , Ganz Olga , Giri Jayeeta , Pennati Andrea , Meyers Ross , Lunga Tadeas , Robbins Daniel J , Thibeault Susan L , Bates Amber M , Nickel Kwangok P , Morris Zachary S , Mattison Ryan J , Chappell Richard , Glazer Tiffany A , Rogus-Pulia Nicole M , Galipeau Jacques , Kimple Randall J 
Published: October 2021

Abstract
Background: Salivary dysfunction is a significant side effect of radiation therapy for head and neck cancer (HNC). Preliminary data suggests that mesenchymal stromal cells (MSCs) can improve salivary function. Whether MSCs from HNC patients who have completed chemoradiation are functionally similar to those from healthy patients is unknown. We performed a pilot clinical study to determine whether bone marrow-derived MSCs (BM-MSCs) from HNC patients could be used for the treatment of RT-induced salivary dysfunction.Methods: An IRB-approved pilot clinical study was untaken on HNC patients with xerostomia who had completed treatment two or more years prior. Patients underwent iliac crest bone marrow aspirate and BM-MSCs were isolated and cultured. Culture expanded BM-MSCs were stimulated with IFNγ and cryopreserved prior to reanimation and profiling for functional markers by flow cytometry and ELISA. BM-MSCs were additionally injected into mice with radiation-induced xerostomia and the changes in salivary gland histology were examined. Results: A total of six subjects were enrolled. BM-MSCs from all subjects were culture expanded to >20 million cells in a median of 15.5 days (range 8-20 days). Flow cytometry confirmed that cultured cells from HNC patients were BM-MSCs. Functional flow cytometry demonstrated that these IFNγ-stimulated BM-MSCs acquired an immunosuppressive phenotype. IFNγ-stimulated BM-MSCs from HNC patients were found to express GDNF, WNT1, and R-spondin 1 as well as pro-angiogenesis and immunomodulatory cytokines. In mice, IFNγ-stimulated BM-MSCs decreased the loss of acinar cells decreased the formation of fibrosis. Conclusions: BM-MSCs from previously treated HNC patients can be expanded for auto-transplantation and are functionally active. Furthermore, IFNγ-stimulated BM-MSCs express proteins implicated in salivary gland regeneration. This study provides preliminary data supporting the feasibility of using autologous BM-MSCs from HNC patients to treat RT-induced salivary dysfunction.Trial Registration: NCT04007081, Registered July 5, 2019, https://clinicaltrials.gov/ct2/show/NCT04007081
Comparative Proteomic Analysis on Chloroplast Proteins Provides New Insights Into the Effects of Low Temperature in Sugar Beet
Authors: Long Jiali , Xing Wang , Wang Yuguang , Wu Zedong , Li Wenjing , Zou Yi , Sun Jiaping , Pi Zhi , Zhang Fushun 
Published: October 2021

Abstract
Background: Low temperature, which is one of the main environmental factors that limits geographical distribution and sucrose yield, is a common abiotic stress during the growth and development of sugar beet. As a regulatory hub of plant response to abiotic stress, activity in the chloroplasts is related to many molecular and physiological processes, particularly in response to low temperature stress. Results: The contents of chlorophyll (Chl) and malondialdehyde (MDA), relative electrical conductivity (REL), and superoxide dismutase (SOD) activity were measured. The results showed that sugar beet could manage low temperature stress by regulating the levels of Chl, REL and MDA, and the activity of SOD. The physiological responses indicated that sugar beets respond positively to low temperature treatments and are not significantly damaged. Moreover, to determine the precise time to response low temperature in sugar beet, well-known abiotic stresses-responsive transcript factor family, namely DEHYDRATION RESPONSIVE ELEMENT BINDING PROTEIN (DREB), was selected as the marker gene. The results of phylogenetic analyses showed that BvDREBA1 and BvDREBA4 were in the same branch as the cold- and drought-responsive AtDREB gene. In addition, the expression of BvDREBs reached its maximum level at 24 h after low temperature by RNA-Seq and qRT-PCR analysis. Furthermore, the changes in chloroplast proteome after low temperature at 24 h were detected using a label-free technique. A total of 416 differentially expressed proteins were identified. GO enrichment analysis showed that 16 GO terms were significantly enriched, particularly chloroplast stroma, chloroplast envelope, and chloroplast thylakoid membrane. It is notable that the transport of photosynthetic proteins (BvLTD, BvTOC100, and Toc-Tic complex), the formation of starch granules (BvPU1, BvISA3, and BvGWD3) and the scavenging of reactive oxygen species (BvCu/Zn-SOD, BvCAT, BvPrx, and BvTrx) were the pathways used by sugar beets to respond to low temperatures at an early stage.Conclusions: These results provide a preliminarily analysis of how chloroplasts of sugar beet respond to low temperature stress at the translational level and provide a theoretical basis for breeding low temperature resistant varieties of sugar beet.
Current Practice of Dutch Hospital-Based Palliative Care Teams: Advance Care Planning in Advanced Stages of Disease
Authors: Doorne Iris , Willems Dick L. , Baks Nadine , Kuijper Jelle , Buurman Bianca. M. , Rijn Marjon 
Published: October 2021

Abstract
Background: Specialist palliative care teams (PCT) are consulted during hospital admission for advice on complex palliative care. These consultations need to be timely to prevent symptom burden and maintain quality of life. Insight into specialist PCTs may help improve the outcomes of palliative care. Methods: In this retrospective observational study, we analyzed qualitative and quantitative data of palliative care consultations in a six-month period in four general hospitals in the northwestern part of the Netherlands. Data were obtained from electronic medical records. Results: We extracted data from 336 consultations. The most common diagnoses were cancer (54.8%) and organ failure (26.8%). 40.2% of patients were restricted to a chair or bed and 52.3% had an estimated life expectancy of less than three months. Within two weeks after consultation, 53.2% of the patients died, and the median time until death was 11 days (range 191) after consultation. Most patients died in hospital (49.4%) but only 7.5% preferred to die in hospital. Consultations were mostly requested for advance care planning (31.6%). End-of-life preferences focused on last wishes and maintaining quality of life. Conclusion: This study shows that palliative care consultations focus on terminal care and are more crisis-oriented than prevention-oriented. Death often occurs too quickly after consultation for end-of-life preferences to be met and these preferences tend to focus on dying. Educating healthcare professionals on when to initiate palliative care would promote a more prevention-oriented approach. Factors that indicate the need for timely PCT consultation should be defined.
Predictive Value of Dosimetric Parameters and Absolute Monocyte Count (AMC) for Acute Hematologic Toxicity in Cervical Cancer Patients Undergoing Concurrent Chemotherapy and Volumetric-Modulated Arc Therapy (VMAT)
Authors: Xiang Xiaoyong , Ding Zhen , Zeng Qi , Feng Lingling , Qiu Chunyan , Chen Dongjie , Lu Jiawei , Li Ning 
Published: October 2021

Abstract
Purpose: To identify clinical/dosimetric predictors of acute hematologic toxicity (HT) in cervical cancer patients undergoing concurrent chemotherapy and volumetric-modulated arc therapy (VMAT).Methods and Materials: We retrospectively analyzed 184 cervical cancer patients receiving concurrent chemotherapy and VMAT. Hematological parameters were collected during the treatment period. The total pelvic bone(TPB) was retrospectively delineated for each patient, and the volume of TPB receiving 10, 20, 30, 40, and 50 Gy (V10, V20, V30, V40, and V50, respectively) was calculated. We assessed the correlations between variables by the Spearman rank correlation test and compared the differences between groups by the Wilcoxon signed-rank test. Binary logistic regression analysis was used to analyze associations between HT and clinical/dosimetric parameters. The receiver operating characteristic curve(ROC) was used to determine the best cut-off values for dosimetric planning constraints.Results: The nadir of absolute monocyte count (AMC) was positively correlated with the nadir of absolute white blood cells (WBC) count (r＝0.5378, 95%CI ＝ 0.4227 to 0.6357, P<0.0001) and the nadir of absolute neutrophil count(ANC) (r＝0.5000，95%CI ＝ 0.3794 to 0.6039, P<0.0001). The decrease and increase of AMC usually occurred before the ANC and WBC. In multivariate logistic regression analysis, the chemotherapy regimen and the TPB_V20 were independent risk factors for developing Grade ≥3 hematologic toxicity. The optimal TPB_V20 cut-off value identified by ROC curves followed by Youden test was 71% (AUC = 0.788; 95%CI, 0.722–0.845; p-value＜0.001).Conclusions: The changing trend of AMC can be used as an effective predictor for the timing and severity of the ANC/WBC nadirs. Maintain TPB_V20 < 71 % and selecting single-agent cisplatin or carboplatin could significantly reduce Grade ≥3 HT in cervical cancer patients undergoing concurrent chemoradiotherapy.
Aquapteridospora Jiangxiensis sp. Nov., A New Aquatic Hyphomycetes From Freshwater Habitats in China and A. Bambusinum Comb. Nov.
Authors: Peng Su-Qin , Liu Yu-Lin , Huang Jun-En , Li Xiao-Hong , Yan Xin-Yi , Song Hai-Yan , Gao Yang , Zhai Zhi-Jun , Hu Dian-Ming 
Published: October 2021

Abstract
During an investigation of freshwater fungi in Jiangxi province, China, a new hyphomycetous species, Aquapteridospora jiangxiensis, was collected and isolated. A. jiangxiensis is characterized by its unbranched and guttulate conidiophores with multi-septa and swollen at the base, polyblastic conidiogenous cells with sympodial proliferations and denticles, guttulate conidia with a sheath. The new species was illustrated and a muti-loci (ITS, LSU, SSU, TEF1 and RPB2) phylogenetic tree was constructed. Pleurophragmium bambusinum was is transferred to Aquapteridospora based on molecular and morphological data. A key to the species of Aquapteridospora is presented in this paper.