bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in polysomnographic, nocturnal penile tumescence and penile doppler ultrasound findings in men with stuttering priapism and sleep-related painful erections
Authors: Johnson Mark , McNeillis Venkata , Gutbier Julia , Eaton Andy , Royston Robert , Johnson Thomas , Chiriaco Giovanni , Walkden Miles , Ralph David 
Published: August 2021

AbstractMen with Stuttering Priapism (SP) and sleep-related painful erections (SRPE) experience bothersome nocturnal painful erections resulting in poor sleep. The aim of this study is to observe common features and differences between men with SP and SRPE based on polysomnography, nocturnal penile tumescence (NPT), and penile doppler ultrasound (PDU). This is a prospective cohort study of 20 participants divided into two groups (Group 1 = SP [n = 12]; Group 2 = SRPE [n = 8]) with bothersome painful nocturnal erections. All participants were referred to the sleep disorder clinic to be assessed and consented for overnight polysomnography with simultaneous NPT recording and to complete validated sleep, sexual dysfunction and health-related quality of life questionnaires. Unstimulated PDU was also performed. Abnormal Polysomnographic findings (reduced sleep efficiency, total sleep time, and awake after sleep onset) were identified in both groups suggesting poor sleep. Men with SP had significantly longer erections (60.0 vs 18.5; p = 0.002) and took longer to detumesce once awake (25.7 vs 5.4 min; p = 0.001) than men with SRPE. They also had significantly higher peak systolic and end diastolic velocities on unstimulated PDU with an abnormal low resistance waveform identified. No sleep pathology was identified in men with SP. This implies a local (penile) etiology in men with SP. Men with SRPE had a normal resting PDU and abnormal sleep architecture with REM awakenings and significantly more Periodic limb movements (p = 0.04) than men with SP suggesting a central (sleep-related) cause in men with SRPE. Sexual dysfunction and poor HR-QoL was identified on validated questionnaires in both groups. SP and SRPE are rare entities that share similar symptoms (painful nocturnal erections and poor sleep) but dissimilar features of nocturnal erection onset, duration and resolution with different polysomnographic features which may allude to a different pathophysiology.
Dzyaloshinskii–Moriya interaction in noncentrosymmetric superlattices
Authors: Ham Woo Seung , Pradipto Abdul-Muizz , Yakushiji Kay , Kim Kwangsu , Rhim Sonny H. , Nakamura Kohji , Shiota Yoichi , Kim Sanghoon , Ono Teruo 
Published: August 2021

AbstractDzyaloshinskii–Moriya interaction (DMI) is considered as one of the most important energies for specific chiral textures such as magnetic skyrmions. The keys of generating DMI are the absence of structural inversion symmetry and exchange energy with spin–orbit coupling. Therefore, a vast majority of research activities about DMI are mainly limited to heavy metal/ferromagnet bilayer systems, only focusing on their interfaces. Here, we report an asymmetric band formation in a superlattices (SL) which arises from inversion symmetry breaking in stacking order of atomic layers, implying the role of bulk-like contribution. Such bulk DMI is more than 300% larger than simple sum of interfacial contribution. Moreover, the asymmetric band is largely affected by strong spin–orbit coupling, showing crucial role of a heavy metal even in the non-interfacial origin of DMI. Our work provides more degrees of freedom to design chiral magnets for spintronics applications.
Allele-specific RT-PCR for the rapid detection of recurrent SLC12A3 mutations for Gitelman syndrome
Authors: Yan Ming-Tso , Yang Sung-Sen , Tseng Min-Hua , Cheng Chih-Jen , Tsai Jeng-Daw , Sung Chih-Chien , Hsu Yu-Juei , Lin Shih-Hua 
Published: August 2021

AbstractRecurrent mutations in the SLC12A3 gene responsible for autosomal recessive Gitelman syndrome (GS) are frequently reported, but the exact prevalence is unknown. The rapid detection of recurrent SLC12A3 mutations may help in the early diagnosis of GS. This study was aimed to investigate the prevalence of recurrent SLC12A3 mutations in a Taiwan cohort of GS families and develop a simple and rapid method to detect recurrent SLC12A3 mutations. One hundred and thirty independent Taiwan families with genetically confirmed GS were consecutively enrolled to define recurrent SLC12A3 mutations and determine their prevalence. Using TaqMan probe-based real-time polymerase chain reaction, we designed a mutation detection plate with all recurrent mutations. We validated this mutation detection plate and tested its feasibility in newly diagnosed GS patients. A total of 57 mutations in the SLC12A3 gene were identified and 22 including 2 deep intronic mutations were recurrent mutations consisting of 87.1% (242/278, 18 triple) of all allelic mutations. The recurrent mutation-based TaqMan assays were fully validated with excellent sensitivity and specificity in genetically diagnosed GS patients and healthy subjects. In clinical validation, recurrent mutations were recognized in 92.0% of allelic mutations from 12 GS patients within 4 h and all were confirmed by direct sequencing. Recurrent SLC12A3 mutations are very common in Taiwan GS patients and can be rapidly identified by this recurrent mutation-based SLC12A3 mutation plate.
The role of genetics in neurodegenerative dementia: a large cohort study in South China
Authors: Jiao Bin , Liu Hui , Guo Lina , Xiao Xuewen , Liao Xinxin , Zhou Yafang , Weng Ling , Zhou Lu , Wang Xin , Jiang Yaling , Yang Qijie , Zhu Yuan , Zhou Lin , Zhang Weiwei , Wang Junling , Yan Xinxiang , Li Jinchen , Tang Beisha , Shen Lu 
Published: August 2021

AbstractNeurodegenerative dementias are a group of diseases with highly heterogeneous pathology and complicated etiology. There exist potential genetic component overlaps between different neurodegenerative dementias. Here, 1795 patients with neurodegenerative dementias from South China were enrolled, including 1592 with Alzheimer’s disease (AD), 110 with frontotemporal dementia (FTD), and 93 with dementia with Lewy bodies (DLB). Genes targeted sequencing analysis were performed. According to the American College of Medical Genetics (ACMG) guidelines, 39 pathogenic/likely pathogenic (P/LP) variants were identified in 47 unrelated patients in 14 different genes, including PSEN1, PSEN2, APP, MAPT, GRN, CHCHD10, TBK1, VCP, HTRA1, OPTN, SQSTM1, SIGMAR1, and abnormal repeat expansions in C9orf72 and HTT. Overall, 33.3% (13/39) of the variants were novel, the identified P/LP variants were seen in 2.2% (35/1592) and 10.9% (12/110) of AD and FTD cases, respectively. The overall molecular diagnostic rate was 2.6%. Among them, PSEN1 was the most frequently mutated gene (46.8%, 22/47), followed by PSEN2 and APP. Additionally, the age at onset of patients with P/LP variants (51.4 years), ranging from 30 to 83 years, was ~10 years earlier than those without P/LP variants (p < 0.05). This study sheds insight into the genetic spectrum and clinical manifestations of neurodegenerative dementias in South China, further expands the existing repertoire of P/LP variants involved in known dementia-associated genes. It provides a new perspective for basic research on genetic pathogenesis and novel guiding for clinical practice of neurodegenerative dementia.
Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
Authors: Lefevre Marie C. , Dijk Gerwin , Kaszas Attila , Baca Martin , Moreau David , O’Connor Rodney P. 
Published: August 2021

AbstractGlioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.
All-weather, natural silent speech recognition via machine-learning-assisted tattoo-like electronics
Authors: Wang Youhua , Tang Tianyi , Xu Yin , Bai Yunzhao , Yin Lang , Li Guang , Zhang Hongmiao , Liu Huicong , Huang YongAn 
Published: August 2021

AbstractThe internal availability of silent speech serves as a translator for people with aphasia and keeps human–machine/human interactions working under various disturbances. This paper develops a silent speech strategy to achieve all-weather, natural interactions. The strategy requires few usage specialized skills like sign language but accurately transfers high-capacity information in complicated and changeable daily environments. In the strategy, the tattoo-like electronics imperceptibly attached on facial skin record high-quality bio-data of various silent speech, and the machine-learning algorithm deployed on the cloud recognizes accurately the silent speech and reduces the weight of the wireless acquisition module. A series of experiments show that the silent speech recognition system (SSRS) can enduringly comply with large deformation (~45%) of faces by virtue of the electricity-preferred tattoo-like electrodes and recognize up to 110 words covering daily vocabularies with a high average accuracy of 92.64% simply by use of small-sample machine learning. We successfully apply the SSRS to 1-day routine life, including daily greeting, running, dining, manipulating industrial robots in deafening noise, and expressing in darkness, which shows great promotion in real-world applications.
Metal–insulator-like transition, superconducting dome and topological electronic structure in Ga-doped Re3Ge7
Authors: Cui Yanwei , Wu Siqi , Zhu Qinqing , Xiao Guorui , Liu Bin , Wu Jifeng , Cao Guanghan , Ren Zhi 
Published: August 2021

AbstractSuperconductivity frequently appears by doping compounds that show a collective phase transition. So far, however, this has not been observed in topological materials. Here we report the discovery of superconductivity induced by Ga doping in orthorhombic Re3Ge7, which undergoes a second-order metal–insulator-like transition at ~58 K and is predicted to have a nontrivial band topology. It is found that the substitution of Ga for Ge leads to hole doping in Re3Ge7−xGax. As a consequence, the phase transition is gradually suppressed and disappears above x = 0.2. At this x value, superconductivity emerges and Tc exhibits a dome-like doping dependence with a maximum value of 3.37 K at x = 0.25. First principles calculations suggest that the phase transition in Re3Ge7 is associated with an electronic instability driven by Fermi-surface nesting and the nontrival band topology is preserved after Ga doping. Our results indicate that Ga-doped Re3Ge7 provides a rare opportunity to study the interplay between superconductivity and competing electronic states in a topologically nontrivial system.
Optical, electrochemical and photocatalytic properties of cobalt doped CsPbCl3 nanostructures: a one-pot synthesis approach
Authors: Bhat Aadil Ahmad , Khandy Shakeel Ahmad , Islam Ishtihadah , Tomar Radha 
Published: August 2021

AbstractThe present manuscript aims at the synthesis of cesium based halide perovskite nanostructures and the effect of cobalt doping on the structural, optical, lumnisent, charge storage and photocatalytic properties. In a very first attempt, we report the solvothermal synthesis of Co doped CsPbCl3 nanostructures under subcritical conditions. The structural features were demonstrated by X-ray diffraction (XRD) Surface morphology determined cubic shape of the synthesized particles. Doping is an excellent way to modify the properties of host material in particular to the electronic structure or optical properties. Incorporation of Co2+ ions in the perovskite structure tunes the optical properties of the nanostructures making this perovskite a visible light active material (Eg = 1.6 eV). This modification in the optical behaviour is the result of size effect, the crystallite size of the doped nanostructures increases with cobalt doping concentration. Photolumniscance (PL) study indicated that CsPbCl3 exhibited Blue emission. Thermogravametric analysis (TGA) revealed that the nanostructures are quite stable at elavated temperatures. The electrochemical performance depicts the pseudocapacative nature of the synthesized nanostructures and can used for charge storage devices. The charge storage capability showed direct proportionality with cobalt ion concentration. And Finally the photocatalytic performance of synthesized material shows superior catalytic ability degrading 90% of methylene blue (MB) dye in 180 min under visible light conditions.
Flood disturbance affects morphology and reproduction of woody riparian plants
Authors: Fischer Sarah , Greet Joe , Walsh Christopher J. , Catford Jane A. 
Published: August 2021

AbstractRiparian forests are structured and maintained by their hydrology. Woody riparian plants typically adapt to the local flood regime to maximise their likelihood of survival and reproductive success. Understanding how extant trees form and reproduce in response to flood disturbance is crucial for predicting vegetation changes and informing restoration. Working in a temperate evergreen riparian forest, we aimed to determine whether disturbance-based responses of plants found in other ecosystems also typify woody plants in riparian forests where disturbances are often mild or chronic, non-lethal, annual events. Using plant surveys and 20-year modelled hydrological data, we examined whether (1) the morphology (main stem diameter, height, crown width, crown extent, stem leaning) and (2) reproduction type (sexual and asexual reproduction) and extent of three dominant woody species (Eucalyptus camphora,Leptospermum lanigerumandMelaleuca squarrosa) vary with flood regime (flood frequency and flood duration); and (3) whether different morphology is associated with different reproductive strategies. Increased flooding generally resulted in increased stem numbers and greater stem leaning—morphologies associated with asexual reproduction—of our study species. More frequent flooding also reduced plant size and sexual reproduction inE. camphora. Sexual reproduction in the studied species was more common in taller plants with single, more upright stems in good condition. Flexible morphology and plastic reproductive strategy may constitute an adaptation of trees to mild or chronic disturbance in floodplains. Our findings suggest that flood regime (i.e. variable frequency and duration of flooding events) is critical to the structural integrity and self-maintenance of species-diverse riparian forests.
Role of interferon regulatory factor 7 in corneal endothelial cells after HSV-1 infection
Authors: Ohtani Fumie , Miyazaki Dai , Shimizu Yumiko , Haruki Tomoko , Yamagami Satoru , Inoue Yoshitsugu 
Published: August 2021

AbstractViral infections of the cornea including herpes simplex virus 1 (HSV-1) cause visual morbidity, and the corneal endothelial cell damage leads to significant visual impairment. Interferon regulatory factor 7 (IRF7) has been identified as a significant regulator in corneal endothelial cells after an HSV-1 infection. To examine the role played by IRF7, the DNA binding domain (DBD) of IRF7 of human corneal endothelial cells (HCEn) was disrupted. An RNAi inhibition of IRF7 and IRF7 DBD disruption (IRF7 ∆DBD) led to an impairment of IFN-β production. Impaired IFN-β production by IRF7 ∆DBD was regained by IRF7 DNA transfection. Transcriptional network analysis indicated that IRF7 plays a role in antigen presentation function of corneal endothelial cells. When the antigen presentation activity of HCEn cells were examined for priming of memory CD8 T cells, IRF7 disruption abolished the anti-viral cytotoxic T lymphocyte (CTL) response which was dependent on the major histocompatibility complex (MHC) class I. To further examine the roles played by IRF7 in CTL induction as acquired immunity, the contribution of IRF7 to MHC class I-mediated antigen presentation was assessed. Analysis of IRF7 ∆DBD cells indicated that IRF7 played an unrecognized role in MHC class I induction, and the viral infection induced-MHC class I induction was abolished by IRF7 disruption. Collectively, the IRF7 in corneal endothelial cells not only contributed to type I IFN response, but also to the mediation of viral infection-induced MHC class I upregulation and priming of CD8 arm of acquired immunity.
Concordance of the spectral properties of dorsal wing scales with the phylogeographic structure of European male Polyommatus icarus butterflies
Authors: Piszter Gábor , Kertész Krisztián , Sramkó Gábor , Krízsik Virág , Bálint Zsolt , Biró László Péter 
Published: August 2021

AbstractThe males of more than 80% of the Lycaenidae species belonging to the tribe Polyommatini exhibit structural coloration on their dorsal wing surfaces. These colors have a role in reinforcement in prezygotic reproductive isolation. The species-specific colors are produced by the cellular self-assembly of chitin/air nanocomposites. The spectral position of the reflectance maximum of such photonic nanoarchitectures depends on the nanoscale geometric dimensions of the elements building up the nanostructure. Previous work showed that the coloration of male Polyommatus icarus butterflies in the Western and Eastern Palearctic exhibits a characteristic spectral difference (20 nm). We investigated the coloration and the de novo developed DNA microsatellites of 80 P. icarus specimens from Europe from four sampling locations, spanning a distance of 1621 km. Remarkably good concordance was found between the spectral properties of the blue sexual signaling color (coincident within 5 nm) and the population genetic structure as revealed by 10 microsatellites for the P. icarus species.
Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)
Authors: Huang Bin , Huang Zhinuo , Ma Ruifang , Chen Jialu , Zhang Zhijun , Yrjälä Kim 
Published: August 2021

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of theHSFgene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bambooHSFgenes could be divided into three major subfamilies;HSFsfrom the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. AllHSFgenes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associatedcis-regulatory elements were identified in theHSFupstream promoter sequences. Synteny analysis indicated that theHSFsin the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bambooHSFgene family. Transcriptome data indicated that the expression of a number ofPeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number ofHSFgenes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bambooHSFgene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.
Impact of water stress under ambient and elevated carbon dioxide across three temperature regimes on soybean canopy gas exchange and productivity
Authors: Singh Shardendu K. , Reddy Vangimalla R. , Devi Mura Jyostna , Timlin Dennis J. 
Published: August 2021

AbstractThe present study investigated the interactive effects of three environmental stress factors elevated CO2, temperature, and drought stress on soybean growth and yield. Experiments were conducted in the sunlit, controlled environment Soil–Plant–Atmosphere–Research chambers under two-level of irrigation (WW-well water and WS-water stress-35%WW) and CO2 (aCO2-ambient 400 µmol mol−1 and eCO2-elevated 800 µmol mol−1) and each at the three day/night temperature regimes of 24/18 °C (MLT-moderately low), 28/22 °C (OT-optimum), and 32/26 °C (MHT-moderately high). Results showed the greatest negative impact of WS on plant traits such as canopy photosynthesis (PCnet), total dry weight (TDwt), and seed yield. The decreases in these traits under WS ranged between 40 and 70% averaged across temperature regimes with a greater detrimental impact in plants grown under aCO2 than eCO2. The MHT had an increased PCnet, TDwt, and seed yield primarily under eCO2, with a greater increase under WW than WS conditions. The eCO2 stimulated PCnet, TDwt, and seed yield more under WS than WW. For instance, on average across T regimes, eCO2 stimulated around 25% and 90% dry mass under WW and WS, respectively, relative to aCO2. Overall, eCO2 appears to benefit soybean productivity, at least partially, under WS and the moderately warmer temperature of this study.
Natural phytoalexin stilbene compound resveratrol and its derivatives as anti-tobacco mosaic virus and anti-phytopathogenic fungus agents
Authors: Song Pengfei , Yu Xiuling , Yang Wenqiang , Wang Qingmin 
Published: August 2021

AbstractPlant diseases caused by plant viruses and pathogens seriously affect crop yield and quality, and it is very difficult to control them. The discovery of new leads based on natural products is an important way to innovate pesticides. Based on the resveratrol is a kind of natural phytoalexin, but it cannot be used as candidate for the development of new drug due to its poor druggability. The phenolic hydroxyl groups in the resveratrol structure are easily destroyed by oxidation, in order to improve its stability, ester formation is the most commonly used modification method in drug design. Their structures were characterized by1H NMR,13C NMR and HRMS. The activity against tobacco mosaic virus (TMV) of these ester derivatives has been tested for the first time. The bioassay results showed part of the target compounds exhibited good to excellent in vivo activities against TMV. The optimum compoundsIII-2(inhibitory rates of 50, 53, and 59% at 500 μg/mL for inactivation, curative, and protection activities in vivo, respectively),III-4(inhibitory rates of 57, 59, and 51% at 500 μg/mL, respectively), andII-5(inhibitory rates of 54, 52, and 51% at 500 μg/mL, respectively) displayed higher activity than commercial plant virucide ribavirin (inhibitory rates of 38, 37, and 40% at 500 μg/mL, respectively). CompoundsI-9andI-10also showed excellent activities. The systematic study provides strong evidence that these simple resveratrol derivatives could become potential TMV inhibitors. The novel concise structure provides another new template for antiviral studies.