bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating Outcome in Perforated Peptic Ulcer by Boey and POMPP Score
Authors: Nuzulistina Elvin , Putro Mamiek Dwi , Fauziah Dyah 
Published: February 2021

Introduction: Perforated peptic ulcer (PPU) is able to increase the risk of mortality and morbidity. This study used Boey and practical scoring system of mortality in patients with perforated peptic ulcer (POMPP) scoring systems to assess risk mortality of the patients. Every parameter has a value to add up 1 point in Boey and POMPP score. Methods: This observational study used medical records of PPU patients who came to Dr. Soetomo General Hospital in emergency state and being operated and treated at surgical inpatient care facility in 2016. The data were analyzed retrospectively. The sampling technique in this study was done by total sampling. Results: Most of PPU patients had the average age of 59.56 years old and 71.79% of the patients were male. Both analyzing results of Boey and POMPP scoring systems were not statistically significant to predict mortality risk of the patients. Even so, the results of Boey scoring system tended to have a positive correlation with mortality risk (0%, 37.50%, 52.94%, and 100%) with 17 patients (43.59%) had mortality. Conclusion: While Boey and POMPP score are most commonly used to predict outcome for PPU patients in Dr. Soetomo General Hospital, considerable variations in risk of mortality were shown. Therefore, both Boey and POMPP score had its own advantages and disadvantages. Further prospective research is needed to test the validity of Boey and POMPP scoring systems, thus the scoring systems can be used in daily hospital practice in patients with PPU.
Correlation between Maternal Infection and Infant Cholestasis
Authors: Mulia Liofelita Christi Adhi , Ranuh I Gusti Made Reza Gunadi , Ernawati Ernawati 
Published: February 2021

Introduction: Infant cholestasis has been associated to viral infection. Cytomegalovirus in the pathogenesis of neonatal hepatitis has been already known and it is one of the possible etiological of biliary atresia. The aim of this study was to outline the correlation between maternal infection and infant cholestasis (aged 0-1 year old) in Department of Pediatrics Dr. Soetomo General Hospital, Surabaya. Methods: A case-control study of pregnant women who gave birth in Dr. Soetomo General Hospital from June 2016 to June 2017. The data were taken from medical records. The cases were defined as pregnant women with babies who had cholestasis, and the control was pregnant women who gave birth to a baby without cholestasis within the age of 0-1 year old. Sample cases in this study were drawn from the total population, with the inclusion criteria babies who had cholestasis, and exclusion criteria that include complications of pregnancy and a history of infant’s parent with genetic disorders. The control variable studied was maternal infection history and sex. Samples were taken by purposive control. The data were obtained then summarized and analyzed by univariate and bivariate, using the chi-square table analysis. Results: The number of cases that met the inclusion criteria were 68 patients, and the amount of control taken was 110 patients. The total number of patients studied was 178 patients. Univariate analysis found the cholestasis was more common in mother with infection during pregnancy (73.53% from the total of cholestasis). Bivariate analyzes found relationship between pregnancy infection and cholestasis (p = <0.001, or = 20.726). Conclusion:The maternal infection is significantly associated with cholestasis in infants.
Autoeficacia de los estudiantes de la carrera traducción inglés-español en instituciones de educación superior chilenas
Authors: Ramirez Solange 
Published: February 2021

Resumen: El presente trabajo corresponde a un estudio exploratorio cuantitativo-cualitativo, de diseño secuencial-explicativo, cuyo objetivo es comprender a nivel a macro y micro la autoeficacia de los estudiantes de la carrera de Traducción inglés-español de instituciones de educación superior chilenas. Para el logro de dicho objetivo, en una primera fase cuantitativa, se aplicó la Escala de Autoeficacia General a 160 estudiantes entre todas las instituciones de educación superior chilenas que imparten la carrera profesional de traducción inglés-español y, para la fase cualitativa subsecuente, se recurrió a una triangulación de las técnicas de protocolo verbal retrospectivo y entrevista en profundidad a 3 participantes de distintas universidades de la V región de Chile. Lo resultados indican que la Escala de Autoeficacia General, a pesar de su frecuente uso en el campo de los estudios de traducción, presenta muy poca especificidad para medir la autoeficacia en estudiantes de traducción inglés-español y, en cuanto a los resultados la etapa cualitativa, los factores que tienen mayor influencia en la autoeficacia de los estudiantes de traducción inglés-español corresponden al manejo disciplinar que estos presentan en las distintas áreas al momento de traducir, al rol que tienen sus docentes y compañeros de curso, y a los distintos estados fisiológicos y emocionales que experimentan.
Palabras clave: Autoeficacia, Estudiantes de traducción, Escala de Autoeficacia General, Traducción, Educación superior.
Biomarkers-based personalized follow-up in chronic heart failure improves patient’s outcomes and reduces care associate cost.
Authors: Justel Antonio Leon , Garcia-Polavieja Jose Ignacio Morgado , Rios Ana Isabel Alvarez , Fernandez Francisco Jose Caro , Merino Pedro Agustin Pajaro , Rios Elena Galvez , Rico Ignacio Vazquez , Fernandez Jose Francisco Diaz 
Published: February 2021

Abstract
BACKGROUND Heart failure (HF) is a major and growing medical and economic problem, with high prevalence and incidence rates worldwide. Cardiac Biomarker is emerging as a novel tool for improving management of patients with HF with a reduced left ventricular ejection fraction (HFrEF).METHODS This is a real-world, before and after interventional study, that assesses the impact of a personalized follow-up procedure for HF on patient’s outcomes and care associated cost, based on a clinical model of risk stratification and personalized management according to that risk. A total of 192 patients were enrolled and studied before the intervention and again after the intervention. The primary objective was the rate of readmissions, due to a HF. Secondary outcome compared the rate of ED visits and quality of life improvement assessed by the number of patients who had reduced NYHA score. A cost- analysis was also performed on these data. RESULTS Admission rates significantly decreased by 65,6% after the intervention, the total hospital admissions were reduced by 41% and the total length of stay was reduced by 46%. The rate of ED visits was reduced by 55%. Thirty-one percent of patients had an improved functional class score after the intervention, whereas only 7.8% got worse. The overall cost saving associated with the intervention was €139,717.65 for the whole group over 1 year.CONCLUSIONS A personalized follow-up of HF patients led to important outcome benefits and resulted in cost savings, mainly due to the reduction of patient hospitalization readmissions and a significant reduction of care- associated costs, suggesting that greater attention should be given to this high-risk cohort to minimize the risk of hospitalization readmissions.
Pressure Change Of Fixed Rotational Deformities In The Femur In Human Cadaver Knees-A Biomechanical Study
Authors: Yuwen Peizhi , Lv Hongzhi , Zhu Yanbin , Chang Wenli , Wei Ning , Guo Jialiang , Wang Haicheng , Ding Kai , Zhang Yingze , Chen Wei 
Published: February 2021

Abstract
Objective: To reveal the contact pressure change on tibial plateau in malalignment femur. Methods: Fourteen cadaveric Lower limbs were selected and autopsied, rotatory fixation model with different angles were then made. Connect each model on the biomechanical machine and apply a vertical load to 400N. The contact pressure was quantitatively measured using ultra-low-pressure sensitive film technology. FPD-305E density meter and FPD-306E pressure converter were used to read relative pressure values. Contact pressure on medial and lateral tibial plateau in different femoral rotational deformities were compared. Analysis were done using SPSS software.Results: The medial group show a significant difference on tibial plateau (F=92.114, P<0.01), further test showed statistically significant differences of pairwise comparisons between 0°, 5°, 10°, 15° internal rotation deformity (P<0.05). There is no significant difference in lateral group (c2=9.967, P<0.01). The medial contact pressure is 0.940±0.177 MPa and the lateral is 1.008±0.219 MPa at neutral position, no statistically significant was found, so is 5° of internal rotational deformity. But the medial contact pressure are all higher than the lateral side at 5°, 10°, 15° of external rotation, and 10°, 15° of internal rotation. Conclusion: Obvious contact pressure changes on tibial plateau were observed in rotatory deformity femur, which is closely related to the occurrence of knee osteoarthritis. Doctors should detect rotational deformity as much as possible during operation and perform anatomical reduction, for patients with residual rotational deformities, indication of osteotomy should not be too broad.
Flow Radiocytometry Using Droplet Microfluidics
Authors: Ha Byunghang , Kim Tae Jin , Moon Eui Jung , Giaccia Amato , Pratx Guillem 
Published: February 2021

Abstract
Flow-based cytometry methods are widely used to analyze heterogeneous cell populations. However, their use for small molecule studies remains limited due to bulky fluorescent labels that often interfere with biochemical activity in cells. In contrast, radiotracers require minimal modification of their target molecules and can track biochemical processes with minimal interference and high specificity. Here, we introduce flow radiocytometry (FRCM) that broadens the scope of current cytometry methods to include radiotracers as probes for single cell studies. FRCM uses droplet microfluidics and radiofluorogenesis to translate the radioactivity of single cells into a fluorescent signal that is then read out using a high-throughput optofluidic device. As a proof of concept, we quantitated [18F]fluorodeoxyglucose radiotracer uptake in single human breast cancer cells and successfully assessed the metabolic flux of glucose and its heterogeneity at the cellular level. We believe FRCM has potential applications ranging from analytical assays for cancer and other diseases to development of small-molecule drugs.
Effects of perioperative managements on ocular surface microbiota in intravitreal injection patients
Authors: Hu Yaguang , Wu Qiong , Sui Fang , Zhang Ming , Zhang Zhen , Shi Rui , Hui Na , Qin Li , Li Li 
Published: February 2021

Abstract
Background: We investigated the effects of perioperative managements on ocular surface microbiota in patients who received intravitreal injections.Methods: Samples of ocular surface microbiota were obtained from 41 eyes of 41 patients who received intravitreal injections at the First Affiliated Hospital of Xi'an Jiaotong University and People’s Hospital in Shaanxi Province. The microbial 16s rDNA from samples were analyzed using an Illumina MiSeq high-throughput sequencing platform. Operating classification units (OTU) clustering and alpha/beta diversity analysis was performed for species classification.Results: High-throughput sequencing yielded 1697337 sequences and 1396, indicating an equivalent number of ocular surface microbiota. OTU was significantly lower in the eyes that received intravitreal injections, compared with those who did not received injections (P<0.05). The number of OTUs was inversely correlated with the administered of perioperative managements and the times of intravitreal injections. Beta diversity showed a significant difference among each groups (P<0.05). With the increased perioperative managements, the prevalence of gram-positive bacteria (Clostridium) had decreased and the prevalence of gram-negative bacteria (Neisseria) had increased.Conclusions: Microbiota on the ocular surface were significantly different between treated and control eyes. And the composition of microbiota on the ocular surface was dramatically changed over time in eyes that received intravitreal injections. Perioperative managements might disrupt the balance of the ocular surface microbiota, increasing risk of infectious disease. Therefore, perioperative managements including local antibiotic eye drops should be applied cautiously before intraocular surgery.
High Efficiency Microwave Absorption Performance of Cobalt Ferrite Microspheres/multi-walled Carbon Nanotube Composites
Authors: Liu Hongmei , Zhang Min , Hu Kang , Kong Xiangkai , Li Qiang , Liu Qiangchun 
Published: February 2021

Abstract
In this article, spinel ferrite CoFe2O4 and multi-walled carbon nanotubes (MWCNTs) composites are constructed by a facile one-step solvothermal method. The pure phase of CoFe2O4 particles is confirmed by X-ray diffraction patterns. Microstructure analysis demonstrates that monodisperse CoFe2O4 microspheres are wound by MWCNTs. By the introduction of CNTs, there is a significant enhancement in the imaginary part of permittivity (ε″) with the composites. The champion microwave absorption performance can be achieved in the composites by the balance of complex permittivity and permeability. When the mass fraction of CNTs is 3%, a minimum reflection loss (RLmin) of the composites is as high as -46.65 dB at 14.4 GHz at a thin thickness of 1.5 mm, and the corresponding effective absorption bandwidth below − 10 dB reaches 4.91 GHz ranging from 12.41 to 17.32 GHz which covers almost the whole Ku band (12.0–18.0 GHz). In other words, this as-synthesized composites show the most outstanding specific RLmin of -31.1 dB•mm− 1. Such superior microwave absorption behaviors of CoFe2O4/CNTs originate mainly from multiple dielectric relaxation processes, enhanced impedance matching and magnetic loss, as well as the considerable interface between mesoporous CoFe2O4 hollow microspheres and CNTs, and thereby promoting microwave reflection and scattering within the samples. Our results indicate that as-fabricated CoFe2O4/CNTs composites can be a promising microwave absorbent integrating with thin thickness, strong absorption ability, and broad bandwidth absorption.
Synbiotic Goat Milk Kefir Lowered Peroxisome Proliferator Activated Receptor Gamma (PPARγ) Gene Expression in Rat Adipose and Liver Tissue
Authors: Nurliyani Nurliyani , Harmayani Eni , Sunarti Sunarti 
Published: February 2021

Abstract
Background

Kefir is a fermented milk product containing bacteria and yeast, whereas glucomannan from porang (Amorphophallus oncophyllus) tuber is known as a prebiotic in vivo. Diets with a high fat and high sugar will stimulate metabolic syndrome associated with changes in gene expression including peroxisome proliferator activated receptor gamma (PPARγ). The purpose of this study was to determine the effect of goat milk kefir enriched with porang glucomannan (synbiotic kefir) and goat milk kefir without glucomannan (probiotic kefir) on blood glucose, hemoglobin A1c (HbA1c), free fatty acid (FFA), tumor necrosis factor alpha (TNF-α), gene expression of peroxisome proliferator activated receptor gamma (PPARγ), and insulin-producing cells in rats fed a high-fat and high-fructose (HFHF) diet.
Methods

Male Sprague Dawley rats 8–12 weeks old (n = 30) treated with HFHF diets for two weeks, and then divided into five dietary groups (each group consisted of 6 rats): 1) normal control (received a standard diet only); 2) rats fed HFHF; 3) rats fed HFHF + probiotic kefir; 4) rats fed HFHF + synbiotic kefir; and 5) rats fed HFHF + simvastatin. The dose of kefir was 3.6 mL/200 g body weight/day and simvastatin was 0.72 mg/day. All of these treatments were carried out for 4 weeks.
Results

There were no significant differences in plasma blood glucose in HFHF rats after and before treatment, but decreased in plasma HbA1c and TNFα (p < 0.05) and inhibited the increase of FFA in rats after synbiotic kefir treatment (paired-samples t-test). Probiotic and synbiotic kefir decreased the gene expression of PPARγ2 (p < 0.05) in both of adipose and liver tissue in HFHF rats but had no effect on the total number of Langerhans islets and insulin-producing cells (one way ANOVA).
Conclusions

Synbiotic kefir could ameliorate the health of rats fed HFHF diet through decreasing HbA1c, TNFα, and PPARγ2 gene expression and preventing an increase in FFA. The results indicate that goat milk synbiotic kefir potentially improve metabolic syndrome.
Rapid Pyrosequencing Method for FMO3 Non-Synonymous Genetic Variant Evaluation in A Korean Population
Authors: Park Jin-woo , Park In-Hwan , Kim Jong-Min , Kim Kyoung-Ah , Park Ji-Young 
Published: February 2021

Abstract
Background: The aim of this study was to develop a feasible pyrosequencing method to detect non-synonymous single nucleotide polymorphisms (SNPs) of the flavin-containing monooxygenase 3 (FMO3) gene and compare the ethnic differences in the frequencies of these alleles. Methods and Results: This pyrosequencing method was used to identify four non-synonymous FMO3 SNPs, including c.855C>T (rs909530), c.441C>T (rs1800822), c.923A>G (rs2266782), and c.472G>A (rs2266782). The allele frequencies of these SNPs in 122 unrelated Korean subjects were analyzed, and were as follows: 44.7% for c.855C>T, 23.4% for c.441C>T, 23.0% for c.923A>G, and 27.1% for c.472G>A. Linkage disequilibrium (LD) analysis showed that c.923A>G and c.472G>A were in strong LD (D′ = 0.8289, r2 = 0.5332). Conclusions: The designed pyrosequencing method was successfully applied to identify the c.855C>T, c.441C>T, c.923A>G, and c.472G>A SNPs. The frequencies were similar to those reported previously in a Japanese population. However, in general, large differences between ethnicities were found.
ZDHHC22-Mediated mTOR Palmitoylation Determines Breast Tumor Growth and Endocrine Therapy Sensitivity
Authors: Huang Jiefeng , Li Jie , Tang Jun , Wu Yushen , Yi Ziying , Wang Yan , Li Yunhai , Tan Yiqing , Wu Yue , Ren Guosheng , Xiang Tingxiu 
Published: February 2021

Abstract
BackgroundPalmitoylation is essential for classic hallmarks of cancer, regulating protein stability and protein-protein interactions. Targeting palmitoylation or palmitoyltransferases has become a novel strategy for therapeutic intervention in cancer. However, few studies have reported the expression pattern, pathological functions and underlying mechanism of ZDHHC22 in breast cancer.MethodsPublic database analysis, quantitative reverse transcription PCR, and western blot were used to analyze ZDHHC22 expression. Methylation-specific PCR was employed to detect the promoter methylation status. Expression of mTOR was examined by immunofluorescence staining and western blots, and palmitoylation of mTOR was detected by acyl-biotin exchange assays. Cellular functions were evaluated via corresponding molecular biological and cellular approaches. Nude mice were used to generate a xenograft tumor model.ResultsWe investigated the expression profiles of 23 palmitoyltransferases using data from The Cancer Genome Atlas and found that ZDHHC22 is frequently expressed at minimal levels in multiple cancers. Downregulation of ZDHHC22 is associated with methylation of its promoter in breast cancer. Ectopic ZDHHC22 expression inhibits the malignant properties of breast cancer both in vitro and in vivo. The enzyme-active site-mutation ZDHHC22-(C111A) mutation reversed these effects, demonstrating that ZDHHC22 suppresses the malignant properties of breast cancer through palmitoylation. Bioinformatics analysis predicted mTOR as the substrate of ZDHHC22. mTOR palmitoylation for the first time was detected and we found ZDHHC22 reduced the stability of mTOR via regulating the palmitoylation of mTOR. Additionally, mTOR expression was frequently negatively correlated with ZDHHC22 in tamoxifen-resistant breast cancer cells and ectopic ZDHHC22 could restore endocrine sensitivity in tamoxifen-resistant breast cancer cell line.ConclusionOur results demonstrating the hypermethylated status and potential tumor suppressor function of ZDHHC22 in breast cancer for the first time established a regulatory mechanism between ZDHHC22 and mTOR palmitoylation, which may pave the way for the development of promising therapeutics that restore endocrine sensitivity.
Immobilization of β-Glucosidase on Different Supports and Evaluation of Its Activity in Cellobiose Hydrolysis
Authors: Silva Karolline Christiny Szeremeta da , Carvalho Yuri , Sousa-Aguiar Eduardo Falabella 
Published: February 2021

Abstract
β-glucosidase was used as catalyst for the hydrolysis of cellobiose, immobilized on different supports, including two silicas (MSNS and MCM-41) and three carbon supports (GAC, BDH and Norit). It was observed that mesoporous nanoparticles are better candidates as supports for the β-glucosidase, and that silica has a better performance, probably due to the presence of silanol groups. The MSNS showed better recovered activity compared to other supports, showing an optimum temperature at 70 0 C and an optimum pH of 5. The enzyme immobilized in MSNS showed results 80% to 98% better than those immobilized on other supports. However, for the recycling test, from the second cycle onwards, the MSNS showed a performance drop of around 40%, reaching a relative activity of 41.9% for both the fourth and the final cycle. MCM-41, on the other hand, did not show as much discrepancy in the recycling test. However, its relative activity in the first cycle was 20% of the activity achieved by MSNS, at its optimum temperature of 60 0 C. Such results indicate that the smaller particle size favours enzymatic activity. Notwithstanding, a larger available intra-pore surface area protects the enzyme from denaturation.
The Inactivation of SARS-CoV-2 in Critical Hospital Settings by Means of Ultraviolet C Lamps: Examples of Their Use and Some Practical Advice
Authors: Lualdi Manuela , Cavalleri Adalberto , Bianco Andrea , Biasin Mara , Cavatorta Claudia , Clerici Mario , Galli Paola , Pareschi Giovanni , Pignoli Emanuele 
Published: February 2021

Abstract
Background

UltraViolet-C (UV-C) lamps may be used to supplement current hospital cleaning and disinfection of surfaces contaminated by SARS-CoV-2. Our aim is to provide some practical indications for the correct use of UV-C lamps.
Methods

We studied three UV-C lamps, measuring their spatial irradiance and emission over time. We quantify the error that is committed by calculating the irradiation time based exclusively on the technical data of the lamps or by making direct irradiance measurements. Finally, we tested specific dosimeters for UV-C.
Results

Our results show that the spatial emission of UV-C lamps is strongly dependent on the power of the lamps and on the design of their reflectors. Only by optimizing the positioning and calculating the exposure time correctly, is it possible to dispense the dose necessary to obtain SARS-CoV-2 inactivation. In the absence of suitable equipment for measuring irradiance, the calculated irradiation time can be underestimated. We therefore consider it precautionary to increase the calculated times by at least 20%.
Conclusion

To use UV-C lamps effectively, it is necessary to follow a few simple precepts when choosing, positioning and verifying the lamps. In the absence of instruments dedicated to direct verification of irradiance, photochromic UV-C dosimeters may represent a useful tool for easily verifying that a proper UV-C dose has been delivered.