bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of mesozooplankton community composition and vertical fluxes in the global ocean
Authors: Soviadan Yawouvi Dodji , Benedetti Fabio , Brandão Manoela C. , Ayata Sakina-Dorothée , Irisson Jean-Olivier , Jamet Jean Louis , Kiko Rainer , Lombard Fabien , Gnandi Kissao , Stemmann Lars 
Published: July 2021

AbstractVertical variations in physical and chemical conditions drive changes in marine zooplankton community diversity and composition. In turn, zooplankton communities play a critical role in regulating the transfer of organic matter produced in the surface ocean to deeper layers. Yet, the links between zooplankton community composition and the strength of vertical fluxes of particles remain elusive, especially on a global scale. Here, we provide a comprehensive analysis of variations in zooplankton community composition and vertical particle flux in the upper kilometer of the global ocean. Zooplankton samples were collected across five depth layers and vertical particle fluxes were assessed using continuous profiles of the Underwater Vision Profiler (UVP5) at 57 stations covering seven ocean basins. Zooplankton samples were analysed using a Zooscan and individual organisms were classified into 19 groups for the quantitative analyses. Zooplankton abundance, biomass and vertical particle flux decreased from the surface to 1000m depth at all latitudes. The zooplankton abundance decrease rate was stronger at sites characterised by oxygen minima (< 5µmol O2.kg−1) where most zooplankton groups showed a marked decline in abundance, except the jellyfishes, molluscs, annelids, large protists and a few copepod families. The attenuation rate of vertical particle fluxes was weaker at such oxygen-depleted sites. Canonical redundancy analyses showed that the epipelagic zooplankton community composition depended on the community structure of surface phytoplankton and the quantity and the quality of the produced particulate organic matter. We provide a consistent baseline of plankton community structure together with estimates of vertical flux and a contribution to our understanding of global zooplankton dynamics in the upper kilometer of the global ocean. Our results further suggest that future changes in surface phytoplankton composition and mesopelagic oxygen loss might lead to profound changes in zooplankton abundance and community structure in both the euphotic and mesopelagic ocean. These changes may affect the vertical export and hereby the strength of the biological carbon pump
Dendrite regeneration in C. elegansis controlled by the RAC GTPase CED-10 and the RhoGEF TIAM-1
Authors: Brar Harjot Kaur , Dey Swagata , Bhardwaj Smriti , Pande Devashish , Singh Pallavi , Dey Shirshendu , Ghosh-Roy Anindya 
Published: July 2021

AbstractNeurons are vulnerable to physical insults which compromise the integrity of both dendrites and axons. Although several molecular pathways of axon regeneration are identified, our knowledge of dendrite regeneration is limited. To understand the mechanisms of dendrite regeneration, we used PVD neurons in C. elegans having stereotyped branched dendrites. Using femtosecond laser, we severed the primary dendrites and axon of this neuron. After severing the primary dendrites near the cell body, we observed sprouting of new branches from the proximal site within 6 hours, which regrew further with timein an unstereotyped manner.This was accompanied by reconnection between the proximal and distal dendrites as well as the fusion among the higher-order branches as reported before. We quantified the regeneration pattern in threeaspects –territory length, number of branchesand fusion phenomena.Axonal injury causes a retraction of the severed end followed by a Dual leucine zipper kinase-1 (DLK-1) dependent regrowth from the severed end.We tested the roles of the major axon regenerationsignaling hubs such as DLK-1-RPM-1, cAMP elevation, let-7 miRNA, AKT-1, Phosphatidyl serine exposure/PS in dendrite regeneration. We found that neither regrowth nor fusionis affected by the axon injury pathway molecules. Surprisingly, we found that the RAC GTPase CED-10and its upstream GEF TIAM-1 play a cell-autonomous role in dendrite regeneration. Additionally, function of CED-10 in epidermal cell is critical for post-dendrotomy fusion phenomena. This work describes a novel regulatory mechanism of dendrite regeneration andprovides a framework for understanding the cellular mechanism of dendrite regeneration using PVD neuron as a model system.
FISH-quant v2: a scalable and modular analysis tool for smFISH image analysis
Authors: Imbert Arthur , Ouyang Wei , Safieddine Adham , Coleno Emeline , Zimmer Christophe , Bertrand Edouard , Walter Thomas , Mueller Florian 
Published: July 2021

AbstractRegulation of RNA abundance and localization is a key step in gene expression control. Single-molecule RNA fluorescence in-situ hybridization (smFISH) is a widely used single-cell-single-molecule imaging technique enabling a quantitative understanding of gene expression and its regulatory mechanisms. Recent progress in experimental techniques provides larger data-sets, requiring adequate tools for data analysis and exploration. Here, we present FISH-quant v2, a highly modular analysis tool accessible both for non-experts and experts, which we validated and applied on large-scale smFISH image datasets. Our package allows the user to detect isolated and clustered mRNA spots, segment nuclei and cells, quantify RNA localization patterns and visualize these results at the single-cell level.
The SARS-CoV-2 reproduction number R0 in cats
Authors: Gonzales Jose L. , de Jong Mart C.M. , Gerhards Nora M. , Van der Poel Wim H. M. 
Published: July 2021

ABSTRACTDomestic cats are susceptible to SARS-CoV-2 virus infection and given that they are in close contact with people, assessing the potential risk cats represent for the transmission and maintenance of SARS-CoV-2 is important. Assessing this risk implies quantifying transmission from humans-to-cats, from cats-to-cats and from cats-to-humans. Here we quantified the risk of cat-to-cat transmission by reviewing published literature describing transmission either experimentally or under natural conditions in infected households. Data from these studies were collated to quantify the SARS-CoV-2 reproduction number R0 among cats. The estimated R0 was significantly higher than 1, hence cats could play a role in the transmission and maintenance of SARS-CoV-2. Questions that remain to be addressed are the risk of transmission from humans-to-cats and cats-to-humans. Further data on household transmission and data on virus levels in both the environment around infected cats and their exhaled air could be a step towards assessing these risks.
Imaging biological samples by integrated differential phase contrast (iDPC) STEM technique
Authors: Li Xujing , Lazić Ivan , Huang Xiaojun , Wang Li , Deng Yuchen , Niu Tongxin , Wu Dongchang , Wirix Maarten , Yu Lingbo , Sun Fei 
Published: July 2021

ABSTRACTScanning transmission electron microscopy (STEM) is a powerful imaging technique and has been widely used in current material science research. The attempts of applying STEM into biological research have been going on for decades while applications have still been limited because of the existing bottlenecks in dose efficiency and non-linearity in contrast. Recently, integrated differential phase contrast (iDPC) STEM technique emerged and achieved a linear phase contrast imaging condition, while resolving signals of light elements next to heavy ones even at low electron dose. This enables successful investigation of beam sensitive materials. Here, we investigate iDPC-STEM advantages in biology, in particular, chemically fixed and resin embedded biological tissues. By comparing results to the conventional TEM, we have found that iDPC-STEM not only shows better contrast but also resolves more structural details at molecular level, including conditions of extremely low dose and minimal heavy-atom staining. For thick sample sections, iDPC-STEM is particularly advantageous. Unlike TEM, it avoids contrast inversion canceling effects, and by adjusting the depth of focus, fully preserves the contrast of relevant features along with the sample. In addition, using depth-sectioning, iDPC-STEM enables resolving in-depth structural variation. Our work suggests that promising, wide and attractive applications of iDPC-STEM in biological research are opening.
Protein kinase Cδ is essential for the IgG response against T cell-independent type 2 antigens and commensal bacteria
Authors: Fukao Saori , Haniuda Kei , Tamaki Hiromasa , Kitamura Daisuke 
Published: July 2021

AbstractAntigens (Ags) with multivalent and repetitive structure elicit IgG production in a T cell-independent manner. However, the mechanisms by which such T cell-independent type-2 (TI-2) Ags induce IgG responses remain obscure. Here we report that BCR engagement with a TI-2 Ag but not with a T cell-dependent (TD) Ag was able to induce the transcription ofactivation-induced cytidine deaminase(AID) and efficient class switching to IgG3 upon co-stimulation with IL-1 or IFN-α. TI-2 Ags strongly induced the phosphorylation of protein kinase C (PKC)δ and PKCδ mediated theAIDtranscription through the induction of BATF, the key transcriptional regulator of AID expression. In PKCδ-deficient mice, production of IgG was intact against TD Ag but abrogated against typical TI-2 Ags as well as commensal bacteria, and experimental disruption of the gut epithelial barrier resulted in fatal bacteremia. Thus, our results revealed novel molecular requirements for class-switching in the TI-2 response and highlighted its importance in homeostatic commensal-specific IgG production.
The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules
Authors: McAlear Thomas S. , Bechstedt Susanne 
Published: July 2021

AbstractCells increase microtubule dynamics to make large rearrangements to their microtubule cytoskeleton during cell division. Changes in microtubule dynamics are essential for the formation and function of the mitotic spindle, and misregulation can lead to aneuploidy and cancer. Using in vitro reconstitution assays we show that the mitotic spindle protein Cytoskeleton-Associated Protein 2 (CKAP2) has a strong effect on nucleation of microtubules by lowering the critical tubulin concentration 100-fold. CKAP2 increases the apparent rate constant ka of microtubule growth by 50-fold and increases microtubule growth rates. In addition, CKAP2 strongly suppresses catastrophes. Our results identify CKAP2 as the most potent microtubule growth factor to date. These finding help explain CKAP2s role as an important spindle protein, proliferation marker, and oncogene.
Pancho’s Burritos
Authors: Dorronsoro Daniel 
Published: July 2021

Pancho’s Burritos is a high-end fast-food restaurant located in Charlottesville, Virginia. Due to a recent surge in customers, the lines at the restaurant during peak hours are becoming very long and causing excessive customer waiting. To improve current customer wait times and plan for future growth requirements, Francisco “Pancho” Escoba, the proprietor, wants to get a better understanding of the current operational capacity. The key decision Escoba must make is how to redesign his burrito-making process to increase the capacity and reduce customer wait time.Suitable for an undergraduate or MBA course, the case can be used in an introductory operations course to teach capacity analysis and queuing. The case provides a relatable and understandable setting for students without an operations background to gain a better grasp of basic course concepts and illustrates how interactions between capacity and queuing affect one another. As an exam or review case it works well because it contains standard process calculations that all students should know how to perform.
A prospective policy transfer of learning from abroad in funding long-term care in England
Authors: Powell Martin 
Published: July 2021

PurposeThis article explores to what extent the long debate in England over the funding of long-term care (LTC) has involved learning from abroad.Design/methodology/approachIt draws on Mossberger and Wolman’s (2003) framework which proposes criteria for assessing policy transfer as a form of prospective policy evaluation: awareness; assessment and application. The documents examined are the sources cited by the reports that examined funding LTC in England since 1999. The study uses interpretive content analysis in a deductive approach (applying the framework) that focuses on both manifest and latent content.FindingsIt finds that both the reports and the cited studies tend to focus on a fairly narrow range of nations, with most attention on Germany, Japan and Scotland. Most studies broadly do not provide much in the way of a clear rationale, and the level of details provided varies. There is relatively little focus on problems. Aims, objectives and goals are little mentioned in some studies, but they tend to be fairly abstract or “high level.” Similarly, there is limited detail on settings. Finally, only a few studies provide a clear recommendation.Originality/valueIt focuses on the neglected topic of the evidence behind reports which are intended to provide recommendations for policy change. The Mossberger and Wolman’s (2003) framework has been used in a small-scale but appears to be well-suited for this purpose.
Relationships among lean, service quality expectation and performance in hospitals
Authors: Vanichchinchai Assadej 
Published: July 2021

Purpose
The purpose of this study is to investigate the links between lean, service quality expectation (SQE) and service quality performance (SQP) in outpatient departments (OPD).

Design/methodology/approach
The measurement instruments were developed from a comprehensive literature review, then verified by experts and confirmatory factor analysis. Multiple responses were applied to improve the reliability of data obtained from 220 Thai hospitals. The hypotheses were tested with linear regression and path analysis of structural equation modeling.

Findings
It was found that lean has significant positive impacts on all SQP dimensions (i.e. tangibility, reliability, responsiveness, assurance and empathy) and on overall SQP. SQE has significant positive direct effects on lean and SQP, and has a significant positive indirect effect on SQP through lean.

Originality/value
This is one of the first examples of research to present insights into the relationships between lean, SQE and SQP in OPD.
Linking knowledge development with sustainable supply chain performance: mediating effects of innovativeness, proactiveness and risk taking
Authors: Tipu Syed Awais Ahmad , Fantazy Kamel 
Published: July 2021

PurposeThe current study provides new insights into the relationships between knowledge development (KD) and sustainable supply chain performance (SSCP) by exploring the mediating effects of entrepreneurial orientation (EO) in terms of innovativeness, proactiveness and risk taking.Design/methodology/approachData were collected by questionnaire survey from 242 manufacturing organizations. Structural equation modeling (SEM) was used to test the hypotheses.FindingsThe results reveal that innovativeness and proactiveness have full mediating effects on the relationship between KD and SSCP. Though KD is negatively related to risk taking and has insignificant indirect effect on SSCP via risk taking, the mediating effect of risk taking remains moderate positive on the relationship between KD and SSCP.Research limitations/implicationsGiven that the current study focuses on manufacturing sector, future research is needed for more comparative studies conducted in different sectors and cultural contexts. The negative link between KD and risk taking also warrants future investigation.Practical implicationsOrganizations may reduce their level of risk taking due to the increase in KD. However, in order to enhance SSCP, risk taking is still needed as it mediates the relationship between KD and SSCP.Originality/valueThe mediating effects of innovativeness, proactiveness and risk taking on the relationship between KD and SSCP are unknown. Current study aims to address this gap.
Inferences on enacted understanding: using immersive technologies to assess intuitive physical science knowledge
Authors: Tscholl Michael , Morphew Jason , Lindgren Robb 
Published: July 2021

PurposeThis study aims to advance the proposal to use immersive virtual learning environments to stimulate and reveal deep-seated knowledge about science, giving instructors and researchers unique possibilities for assessing and identifying intuitive physical science knowledge. Aside from the ability to present rich and dynamic stimuli, these environments afford bodily enactment of people’s understanding, which draws less from declarative knowledge stores and more from everyday experiences with the physical world.Design/methodology/approachThe authors ground their proposal in a critical review of the impact of stimulus and task characteristics of traditional physics inventories. Using a grounded theory approach, the authors present classifications and interpretations of observed bodily enactments of physics understandings in a study where participants enacted their understanding of force and motion of space in an immersive, interactive mixed reality (MR) environment.FindingsThe authors find that instances of these action categories can be interpreted as relating to underlying knowledge, often identified by other studies. The authors thus replicate a number of prior findings, which provide evidence to establish validation for using MR simulation as a tool for identifying people’s physical intuitions.Research limitations/implicationsThis study targeted only a few specific physical science scenarios. Further, while a number of key insights about student knowledge came from the analysis, many of the observations are mere leads in need of further investigation and interpretation rather than core findings.Originality/valueImmersive digital learning environments are primarily used for instruction. The authors propose to use and design them for assessment as well. This paper should prompt more research and development in this direction.
When to choose market foreclosure and vertical mergers with substitutable final products
Authors: Zhang Huailiang , Zhou Yan , Jiang Minghui 
Published: July 2021

PurposeBased on the idea of part standardisation and product differentiation in lean management, this paper answers the question when integrate firms should choose market foreclosure to maximise profits by studying a two-tier supply chain, which contains three types of firms: suppliers, manufacturers and integrated firms. Moreover, the effect of the substitutability between final products and the competition among firms in the supply chain would be investigated from the perspective of dynamic analysis.Design/methodology/approachConsidering the decision order of integrated firms and manufacturers in the downstream of the supply chain, the authors build three competition models. In each model, integrated firms compete with manufacturers in Bertrand–Nash fashion. And, suppliers compete with each other in Cournot fashion, so do integrated firms and manufacturers. The authors further discuss how the competitive relationship between firms affect the equilibrium result.FindingsNumerical analysis reveals that under other conditions unchanged, the increased competition between downstream firms leads to the rise in the willingness of selling parts for integrated firms, while the increase in the number of suppliers has the opposite effect. In addition, due to the market change before and after the vertical merger, it may lead to the transition from profitable to unprofitable for the vertical merger.Originality/valueThis paper provides a theoretical analysis and managerial implication for integrated firms' market foreclosure decision. From the perspective of dynamic analysis, this paper demonstrates the result of vertical mergers and provides an explanation for the failure of vertical mergers.
The demoralization of society and the proliferation of organization
Authors: Rachlitz Kurt , Grossmann-Hensel Benjamin , Friedl Ronja 
Published: July 2021

PurposeIn this paper, the authors aim to clarify the relationship between organization and society. They argue that the proliferation of organization in modernity has not yet been properly understood in light of the absence of organization in premodern times. The authors therefore ask: Why do organizations proliferate? Why do they proliferate in such manifold organizational forms? And how can these heterogeneous forms nevertheless be related to a common problem to which organizations provide a solution? A comparative historical analysis based on the theory of social systems reveals that organizations fill a gap which the decline of morality as an integrative success medium created.Design/methodology/approachThe paper develops a conceptual framework focusing on the theory of media within Luhmann’s theory of social systems as a point of departure. The authors discuss the concept of “interpenetration” to assess the relation between morality and organization. They raise several follow-up questions for future empirical research, most prominently pertaining to the relationship between organization and digitalization.FindingsThe main finding is that morality can be conceptualized as a specific success medium (alongside religion and symbolically generalized communication media) which used to structure premodern societies by means of social and interhuman interpenetration at once. Modern society instead employs two differentiated forms of interpenetration: Social interpretation through organizations and interhuman interpenetration through love relationships. These centripetal counterforces help to mediate the centrifugal forces unleashed by the full development of modern success media. Modern society critically depends on the proliferation of organizations.Originality/valueThis paper examines the relationship between morality and organization not from the perspective of interaction or organization, but from the perspective of society. This approach provides novel insights in that it opens up promising avenues of comparison between organization and other social forms. Understanding the distinctively modern “success story” of organization as a social form makes it possible to ask about corresponding potentials and limitations, but also alternative possibilities. In doing so, the authors depart from most studies of organizations grounded in social systems theory as the authors primarily focus on Luhmann’s theory of media (as opposed to the theory of differentiation).