bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dipeptidyl Peptidase IV as a Novel Prognostic Marker and Important Therapeutic Target in Melanoma
Authors: Piatkowska-Chmiel Iwona , Gawronska-Grzywacz Monika , Iwan Magdalena , Natorska-Chomicka Dorota , Herbet Mariola , Korga-Plewko Agnieszka , Dudka Jaroslaw 
Published: July 2021

Abstract
BackgroundThere is a lot of evidence which suggests that DPP IV level may correlate with a type of tumor cells, metastatic potential and prognosis for the patient. Bearing in mind that the melanomas are characterized by high heterogeneity and identification of specific phenotypes of cells allows for early and more effective therapy, the aim of our study was to check whether there is a correlation between the DPPIV and the metastatic potential of melanoma cell lines. Additionally, the aim of our research was to evaluate the anti-tumor potential of linagliptin and saxagliptin in melanoma cell lines as well as determining correlation between cytotoxicity of the drugs and DPP IV level. MethodsThe inhibitory effect of tested drugs on the cancer cell growth was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) while cell cycle analysis and apoptosis were performed using the NucleoCounter® NC-3000™ system (ChemoMetec, Denmark), following the instructions provided by the manufacturer. DPPIV release by cancer cells was measured by DPP4/CD26 ELISA assay kit for biological samples (Cloud-Clone Corp.,Wuhan,China). ResultsOur results showed that DPPIV overexpression promoted cell proliferation of melanoma cells. Our data showed that especially short term treatment with linagliptin is associated with not only decreased expression of DPPIV and inhibition of cell proliferation but also induction of cell cycle disruption and apoptosis in melanoma. Conclusions﻿The routine identification of this glycoprotein in melanoma would be fundamental to assessing not only the risk of metastasis/disease progression but also selection of therapy and evaluation of its effectiveness.
Without Moths or Bats, Cacti form Friendships with Bees: Reproductive Biology of the Queen of Night Selenicereus Setaceus (Cactaceae) in São Thomé Das Letras, Brazil
Authors: Sampaio Paulo S.P. , de Almeida Odair J.G. 
Published: July 2021

Abstract
Epiphyte, hemiepiphyte and scandent cacti comprise about 10% of the total species of Cactaceae and are the least studied group in the family. Despite the close relationship of Selenicereus setaceus with the well-known and internationally traded pitahayas / dragon-fruit crops, researchers have only recently increased their study of this species. Selenicereus setaceus is the only species of the genus that occurs in the southern American continent; the genus has its center of diversity in Central America and Mexico. Experiments using exclusion methods were carried out to monitor reproductive success and determine which floral visitors were effective pollinators of S. setaceus in southeastern Brazil. Pollination treatments were carried out to determine the reproductive system of the species in addition to observations to unveil the phenology and quantify the volume and concentration of sugars of the nectar produced during anthesis. The Apis mellifera bee was the most frequent visitor and the main pollinator of the S. setaceus flowers. The studied population is self-compatible and fruits with manual or spontaneous self-pollination through physical contact between the anthers and the stigma lobes during flower closing. The nectar had an average sugar concentration of 28.3% and an average volume of 133µl. Although the floral characteristics fit into the sphingophily and chiropterophily syndromes, natural pollination occurred mainly from daytime visitation by A. mellifera, which promotes both self-pollination and cross-pollination mainly during the flower closing period between 5:00 am and 10:00 am. In addition, spontaneous self-pollination also contributed to increasing the rate of fruiting, since visits by nocturnal pollinators were rare.
Synergistic Inhibitory Effect of Maleic Anhydride Derivatives and Quercetin on Cervical Cancer Cells Mediated by Attenuating Cell Survival through Modification of the GSH System
Authors: TORRES GABRIELA CARRASCO , Millán-Casarrubias Elmer Joel , Monroy-Ramírez Hugo Christian , Trujillo-Ferrara José Guadalupe , Vásquez-Garzón Verónica Rocío , Villa-Treviño Saúl 
Published: July 2021

Abstract
Oxidative stress is considered apromotive factor in cervical cancer progression. Agents that decrease cellular GSH levels have been reported to modulate the proliferation rate of cancer cells. To explore a new therapeutic candidate for cervical cancer, we evaluated the effects of combined administration of a maleic anhydride derivative and quercetin on a human cervical cancer-derived (HeLa) cell line and human epithelial cell lines (HaCaT, THLE-3 and cultured primary cells). The combinatorial treatment sensitizes tumor cells through significant decreases in ROS and GSH levels, inducing apoptotic cell death, which was probably induced by disruption of cellular redox homeostasis. In addition, S-phase cell cycle arrest, decreased cell migration and clear evidence of changes in cytoskeletal actin and core morphology were observed in HeLa cells at 24 and 48 h after treatment. In an in vivo model, we observed an increase in the survival time of animals given the combination treatment, as well as tumor inhibition. Therefore, current data suggest that this alteration in the cellular redox state mediated through coadministration of established agentsmay be a promising chemotherapeutic approach.
Glucocorticoid and Antibiotic Treatment-induced Recapitulated Hematological Remissions in Acute Myeloid Leukemia: Implications for Ligand-dependent Growth and Survival Advantage
Authors: Sun Xiao-Yun , Xiao Shu-Xin , Yang Xiao-Qiu , Yang Xiao-Dong , Meng Fan-Jun , Zhao Xi-Chen 
Published: July 2021

Abstract
Leukemia-transformed multipotential hematopoietic cells have acquired the increased self-renewal capacity and impaired myeloid differentiation to mature blood cells. The emergence of symptomatic leukemia also critically requires the acquisition of growth and survival advantage in leukemic cells. Untreated leukemia patients usually demonstrated a progressive process. However, spontaneous remission occasionally occurred in a very small number of leukemia patients, which frequently followed a febrile episode and antibiotic treatment and was generally attributed to the activation of anti-neoplastic activities. Here we report a 63-year-old Chinese man who presented with the main complaints of abdominal pain, febrile episode and urticaria-like skin lesions. He was diagnosed with acute myeloid leukemia (AML-M4) with t(8;21)(q22;q22)/RUNX1-RUNX1T1 on the basis of morphological, immunological, cytogenetic and molecular analysis. He also had a mutated FLT3-TKD gene. He was treated with antibiotics and glucocorticoid for the gastrointestinal infection and the urticaria-like skin lesions. The infection and skin lesions were quickly resolved. Unexpectedly, along with the relief of the febrile episode, abdominal symptoms and skin lesions, he achieved a hematological remission. After relapse, repeating this treatment resulted in the second hematological remission. These recapitulated treatment responses strongly suggested that inflammatory stresses arising from the gut inflammatory lesions, which could be largely mitigated by antibiotic and glucocorticoid treatment, sustained the growth and survival advantage of the leukemic cells.
The Diagnostic Value of Adenosine Deaminase in Children With Infectious Mononucleosis by EBV
Authors: Shi Ting , Li Jungen , Miao Yuzhu , Huang Linlin , Tian Jianmei 
Published: July 2021

Abstract
Background

Infectious mononucleosis (IM) which was a common disease in children was often accompanied by elevated transaminase and occasionally liver failure. This study aimed to explore the diagnostic value of adenosine deaminase in IM and its concurrent elevated alanine aminotransferase (ALT).
Methods

104 children with IM and 50 controls with acute infection, of whom main symptoms were fever, tonsillitis or lymphadenitis, from Children’s Hospital of Soochow University were enrolled in this study. Among 104 children with IM, 54 had normal ALT (IM1 group) and 50 had elevated ALT (IM2 group). Then the clinical and laboratory data of the children were collected to explore the diagnostic value of ADA in the three groups.
Results

ADA in the IM group was significantly higher compared with it in the control group (P < 0.001). ADA had a high correlation with lymphocyte count, CD3 + CD8 + T cell (%), CD4+/CD8 + and CD3-CD19+(%) (|r| >0.7, p < 0.01). ADA had great diagnostic value in pediatric patients with IM. The sensitivity and specificity of ADA in predicting pediatric IM patients were 97.1% and 94.0%, respectively. Furthermore, multivariate regression analysis indicated that ADA was risk factor for elevated ALT in children with IM.
Conclusions

ADA may be a novel diagnostic indicator for pediatric IM and indirectly predict elevated ALT.
Seasonal heat stress alters the milk yield, antioxidative levels and serum metabolites in primiparous and 3 multiparous dairy cows
Authors: Chen Xue , Dong Jia Nan , Rong Ji Ye , Xiao Jun , Zhao Wei , Aschalew Natnael D. , Zhang Xue Feng , Wang Tao , Qin Guixin , Sun Zhe , Zhen Yuguo 
Published: July 2021

Abstract
This study aimed to explore the seasonal heat stress affects the milk yield, antioxidative levels and serum metabolites in primiparous and multiparous dairy cows during early lactation. A total of two hundred dairy cows were selected according to their calving months (June, temperature humidity index (THI) =66.72; July, THI=70.30; August, THI=69.32; September, THI=67.20; October, THI=59.45). Blood samples were collected on day 0, 21, 50, 80, 100 after calving for serum oxidative status analysis and milk yield was recorded every day. The lower average daily milk yield in cows that calved in June and July (P<0.05), and average daily milk yield of multiparous cows was higher than that of primiparous cows that calved in the same month (P<0.05), suggesting that seasonal (June) heat stress negatively affected milk yield in both primiparous and multiparous cows at early lactation. Besides, 15 and 11 serum metabolites were changed in heat stress (average THI = 70.30) group compared with non heat stress (average THI = 59.45) group in primiparous cows and multiparous cows, respectively. These metabolites were mainly involved in the pathways of aminoacyl-tRNA biosynthesis, glyoxylate and dicarboxylate metabolism, and the metabolism of glycine, serine and threonine. These data suggested that heat stress negatively affected the milk yield and thus caused the the elevation of 2 the serum oxidative and antioxidative index Metabolic biomarkers associated with the heat stress 29 in serum were found 0 which providing the basement of evaluating indicator between heat stress and non heat stress groups in primiparous and multiparous cows.
Exclusive Breastfeeding Practice Among Lactating Mothers Infected with HIV in Southern Highlands of Tanzania, Assessing the Prevalence and Factors Associated with the Practice; An Analytical Cross-Sectional Study
Authors: Moshi Fabiola Vincent , Faustine Rose 
Published: July 2021

Abstract
Background: There is no other better way proven to safeguard an infant's health in the first six months of life than Exclusive Breast Feeding (EBF). Mother's breast milk is valuable in all aspects of an infant's physical and mental growth as well as immune development. The study aimed at assessing the prevalence and factors associated with EBF practice among lactating HIV-infected mothers in the Southern Highlands of Tanzania. Method: A hospital-based analytical cross-sectional study was conducted among lactating HIV-infected mothers. A random sampling procedure was used to obtain 372 HIV-infected mothers of infants of 6 to 12 months who were still breastfeeding at the time of data collection. An interviewer-administered structured questionnaire was used for data collection. Bivariate and multivariate logistic regression was used to assess factors associated with EBF practice. Statistical package for social science (SPSS v.20) software was used for data entry and analysis. Results: The prevalence of EBF practice was 58.1% at 95% Confidence Interval of 52.9% to 63.1%. More than half of the respondents 199(53.5%) had adequate knowledge while 173(46.5%) had inadequate knowledge on EBF. After adjusted for confounders, factors associated with EBF practice were knowledge on EBF [Adequate knowledge (AOR=5.114 at 95% CI= 3.2-8.172, p<0.001)], ANC visit [ Adequate (AOR=1.756 at 95% CI=1.094-2.817, p=0.002)], Income per day [1 0r more USD (AOR=1.828 at 95% CI=1.136-2.943, p=0.013)], perception towards EBF [ positive perception (AOR=3.506 at 95% CI=2.248-5.468, p<0.001) and ever experienced breast problem AOR=3.908 at 95% CI=1.891-8.075, p<0.001 Conclusion: More than half of interviewed mothers practiced EBF. The EBF practice among HIV lactating mothers was significant influenced by adequate knowledge on EBF, positive perception toward EBF, adequate ANC visits, and having never experienced breast problems. Strengthening adherence to ANC routine visits, counseling on breastfeeding, and improving mother's knowledge of exclusive breastfeeding would contribute to the enhancement of EBF practice in this region. An innovative interventional study is recommended to come up with an effective strategy to improve EBF knowledge among HIV-infected mothers.
Realization of Twenty Monochromatic Beams using Photonic Structure via Principle of Filtering
Authors: Swain K.P. , Das Subhankar , Samal Soumya Ranjan , Sahu Sanjay Kumar , Palai Gopinath 
Published: July 2021

Abstract
The current work employs silicon-based one dimensional photonic structure which delivers ‘20’ different types of monochromatic beams (wavelengths) via filtering action. The I/P signals are essentially varies from visible to short infrared range to justify the work. Though similar type of works related to filtering application are found in the literature, the present research deals with an output signal which could be deployed in different purposes vis-à-vis dentistry, dermatology, spectroscopy, printing, holography, barcode scanning etc. The physicality of this work incorporates 68 layers of silicon monoxide and silicon based one-dimensional optical waveguide along with their configuration where the plane wave expansion method does fulfill the nitty-gritty of required mathematics to solve out electromagnetic wave equations. Reflectance and transmittance characteristics along with the absorbance are the critical parameters that substantiate the said application.
Icosahedron [Cl12]12- Templated Gigantic Gd158Co38 Nanocluster with the Largest Ln158 Core for Magnetic Cooling
Authors: Liningfang Li , Luo Ximing , Wang Jia , Wang Jilei , Song You , Mei Hua , Xu Yan 
Published: July 2021

Abstract
The synthesis of large nano-sized cluster-molecules is a goal that synthesists and structural scientists have been pursuing, as well as a huge challenge. Herein, the largest 3d-4f metal clusters Cl12@Gd158Co38 and Br12@Gd158Co38 until now are obtained through the “multi-anions-template” strategy, with a protein-sized metal frame (ca. 4.3 × 3.6 × 3.5 nm3). Different from the mixed distribution of 3d and 4f metals and the hollow structure in the previous giant 3d-4f clusters, for the dense core-shell structure Cl12@Gd158Co38 and Br12@Gd158Co38, the Ln158 core with the highest Ln nuclearity number is induced by icosahedra-shaped templates [Cl12]12- or [Br12]12-, while 3d metals (Co) are distributed on its periphery. Their appearances point out a new structure type of non-open giant Ln-based clusters (metal number > 100) and provide an ideal model for studying the multi-level assembly of complex macromolecules. Additionally, Cl12@Gd158Co38 shows the largest magnetic entropy change (-∆Smmax = 46.95 J kg-1 K-1 under 2.0 K and ΔH = 7 T) among reported high-nuclearity 3d-4f clusters.
High dose vitamin C in sepsis (HDVCIS): study protocol for a randomized controlled trial
Authors: Zhao Bing , Li Mengjiao , Li Jian , Liu Leshan , Wang Yihui , Sun Silei , Xu Lili , Qi Xing , Xie Mengqi , Zhou Yuhua , Ni Tongtian , Yao Yi , Chen Peili , Yu Meiling , Jiang Weisong , Wang Ruilan , Tong Chaoyang , Cao Yu , Sheng Huiqiu , Mao Enqiang , Chen Erzhen 
Published: July 2021

Abstract
Sepsis is an inflammatory syndrome with life-threatening organ dysfunction resulting from a dysregulated host response to infection. Although the treatment for sepsis has improved a lot in the last 30 years, sepsis related mortality remains high. In the recent 10 years, high dose intravenous injection of vitamin C, the first line antioxidant of human, has received more and more attention in the field of critical care, its beneficial effect has been demonstrated by several small scale clinical trial and animal studies, but the effect of high dose vitamin C on sepsis in a larger trial seems warranted in further study. Here we will conduct a prospective, multi-center, double-blind, adaptive sample size, randomized, placebo-controlled, clinical trial named as HDVCIS. The trial protocol has been approved by Clinical Trail Ethics Committee of Ruijin Hospital of Shanghai JiaoTong University School of Medicine. This trial has been registered in Chinese Clinical Trial Registry (ChiCTR1800017633) in August 7, 2018. Patients will be recruited from 4 medical centers in China. Its object is to testify the efficacy and safety of high dose vitamin C in the treatment of sepsis.
A New Measurement Technique for Location Before Osteotomy of The Distal Femur in Patients Undergoing Total Knee Arthroplasty
Authors: Zhao Xuequan , Zhao Bin , Yao Shuzhang , Cao Kanduo , Hu Xueliang , Ren Chongxi 
Published: July 2021

Abstract
Background

Many traditional methods are available to prevent unbalance of extension and flexion gap during total knee arthroplasty (TKA), but there are no reports on the use of measurement and positioning method before tibial osteotomy with self-made tools.We designed a self-made tool measuring the location before tibial osteotomy and determined the clinical effect.
Methods

The retrospective study included patients who received TKA at our hospital, between January 1, 2012 and December 31, 2015. A new method, named as the measurement and localization before osteotomy with self-made tools (MOST), was developed to measure the osteotomy position of the posterior femoral condyle during TKA. They were divided into two groups, one that received the new method (GroupⅠ), and the other that received the traditional method as a control (GroupⅡ). HSS score, Oxford score, VAS score and knee joint activity were evaluated in two groups.
Results

One hundred and eighty-seven of 210 eligible patients were included. The function of knee joint in all patients was improved and the pain was obviously relieved. Significant differences were found in the HSS score, Oxford score, VAS score, knee joint activity between two groups at 5-year follow-up (p < 0.05).
Conclusions

The use of MOST may contribute to improve the balance between flexion and extension gaps as well as the balance between internal and external gaps during TKA, and overcome knee flexion instability.
Oleanolic acid mediated the proliferation and invasion of U251 glioma cells and promoted their apoptosis through the IKK-β, MAPK3, and MAPK4 signaling pathway
Authors: Huang Jinxiang , Lin Shengnan , Zhu Feng , Chen Dengsheng , Huang Fang , xu luning 
Published: July 2021

Abstract
ObjectTo investigate the effects of Oleanolic acid (OA) on proliferation, apoptosis, migration, and invasion of human glioma cell U251, as well as IKK-β and MAPK signaling pathways.MethodsThe binding of OA to IKK-β and MAPK signaling pathway essential proteins IKK-β, MAPK3, and MAPK4 was analyzed by molecular docking technique. U251 cells were treated with different concentrations of OA. The proliferation and apoptosis rates of U251 cells were detected by CCK-8 assay, MTT assay, cell cloning assay, and AnnexinⅤ FITC/PI double staining assay. Transwell chamber assay was used to detect migration and invasion of U251 cells. Finally, Western blotting was used to detect the protein expression levels of IKK-β, MAPK3, and MAPK4 in U251 cells treated with OA.ResultsThe results of molecular docking showed that OA could stably bind to IKK-β, MAPK3, and MAPK4 proteins. OA could not only effectively inhibit the proliferation and induce apoptosis of U251 cells (P < 0.05), but also significantly inhibit the invasion of U251 cells (P < 0.05). Western blot assay confirmed that OA could dramatically inhibit the protein expression levels of IKK-β, MAPK3, and MAPK4 in U251 cells (P < 0.05).ConclusionsOA may inhibit the proliferation, migration, and invasion of glioma U251 cells by binding key molecules of the IKK-β signaling pathway and essential target proteins of MAPK3 and MAPK4 in the MAPK signaling pathway.
Gelidocalamus Fengkaiensis (Poaceae: Bambusoideae), A New Bamboo Species From Guangdong, China, With An Analysis of Branch Development in Relation To Flowering
Authors: Cai Zhuo-yu , Xin-Xin Zhou , Wong Khoon-Meng , Xia Nian-He 
Published: July 2021

Abstract
Background: Bamboos, widely distributed in temperate and tropical Asia, Africa and America, refer to a group of special plants in Poaceae, Bambusoideae. China is rich in bamboo species. However, due to long flowering cycle, the flowering habit and the flowering structure of many bamboo species are still unknown. Here, we report a new bamboo species from Guangdong, China and an analysis of its interesting branch development in relation to flowering.Results: This species is similar to G. stellatus, the generic type, but differs in the characteristics of its lemma and palea, mid-culm branch complement, and culm-sheath ligules. The initial branches at a culm node do not apically develop flowering structure during a flowering episode; instead, these form on what appears to be specialized flowering branches.Conclusions: The results of morphological comparison support Gelidocalamus fengkaiensis recognized as a new species. And during flowering episode, two branch types (‘foliage branch’ and ‘flowering branch’) can be distinguished in this species.
Selegiline Improves Excessive Daytime Sleepiness in Parkinson’s Disease: An Open Trial
Authors: Zhang Jinru , Chen Ju-ping , Li Jia , Li Jian , Miao Hong , zhu xiangyang , meng meng , han Yang , chen Jing , Cheng Xiaoyu , Xiong Kang-ping , Jin Hong , luo Wei-feng , Mao Cheng-jie , Liu Chun-Feng 
Published: July 2021

Abstract
Excessive daytime sleepiness (EDS) is common in Parkinson’s disease (PD) and may respond to dopaminergic therapies. The aim of this open-label trial was to assess the efficacy and safety of selegiline as a treatment for EDS in PD patients. In this multi-center study, 141 PD subjects were enrolled between December 2019 and October 2020, and 121 patients completed the 8-week study. Selegiline was administered at 5 mg/day in the first week and was increased to 10 mg per day during the following weeks. EDS was evaluated using the Epworth Sleepiness Scale (ESS) at baseline, week 3, and week 8. Changes in motor complications, sleep quality, and quality of life were also assessed in patients with PD. As primary outcomes, ESS scores decreased significantly in PD patients who received selegiline for 8 weeks (P < 0.001). As secondary outcomes, the scores of Parkinson's disease sleep scale (PDSS) and Parkinson’s disease quality of life questionnaire 39 (PDQ-39) also improved after the treatment. The severity of motor fluctuation improved (P=0.037), while the percentage of patients with dyskinesia and the severity of dyskinesia did not increase significantly compared with baseline. Participants treated with selegiline showed an improvement in EDS and good tolerance of the drug. This study demonstrated the efficacy of selegiline for the treatment of daytime sleepiness in PD patients.Trial registration: clinicaltrials, NCT04870372. Registered 5 May 2021 - Retrospectively registered, https://register.clinicaltrials.gov/prs/app/action/SelectProtocol?sid=S0009J06&selectaction=Edit&uid=U00026CP&ts=2&cx=ruz8uy.