bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and antimicrobial activity test of endophytic fungi from water hyacinth petiole (Eichhornia crassipes) against Escherichia coli and Staphylococcus aureus
Authors: Fanani A Z , Triastuti J , Sulmartiwi L 
Published: April 2021

Abstract
Endophytic fungi have bioactive compounds in the form of secondary metabolites that come from reciprocal relationships with their host plants. Microorganisms such as endophytic fungi has important role in the production of bioactive compounds in the field of biotechnology. The main goal of this study was to find out the antimicrobial activity of endophytic fungi from water hyacinth petiole against E. coli and S. aureus. The endophytic fungi were cultured from petiole of water hyacinth on PDA for 4-5 days at 30ºC. The fungi isolates that have grown were purified and used to test of antimicrobial activity. The method used for antimicrobial test is overlay and disc diffusion agar test. There were 8 endophytic fungi isolated, a total of 4 endophytic fungi identified. Overlay test of the results showed only three isolates had antimicrobial activity i.e Aspergillus sp. Trichophyton sp. and Penicillium sp. Disk diffusion test of the results showed that Penicillium sp. had strong antimicrobial activity with inhibition zones 13.6 ± 1.4 mm (E. coli) and 7.5 ± 1.1 mm (S. aureus). Meanwhile, Aspergillus sp. produced antimicrobial activity with inhibition zones 2.5 ± 0.2 mm and Trichophyton sp. of 2.8 ± 0.7 mm against E. coli bacteria.
Prevalence and intensity of endoparasitic helminth on swamp eel (Monopterus albus) from natural caught and cultivation
Authors: Januarista Y T , Kismiyati  , Ulkhaq M F 
Published: April 2021

Abstract
Swamp eel (Monopterus albus) is one of the highly prospect freshwater fisheries commodities that are consumed by broad community. Marketed swamp eels are generally derived from natural caught and cultivated eels. Natural caught swamp eel has high risk of infected parasites from a natural feed that contaminates with larvae of the parasite of cultured swamp eel could be infected with a parasite from poor pond biosecurity. Information about the types of parasites that infect swamp eels from natural caught and culture has never been reported before. The aims of these studies were to identify the endoparasite and calculate the prevalence and intensity of endoparasite in swamp eel from natural caught and cultivation. A total of one hundred and twenty swamp eel (37.7±2.5 cm height) collected from natural caught and pond culture in Banyuwangi. Two endoparasites helminth were infected to eels from natural caught and cultivation i.e Eustrongylides ignotus and Pingus sinensis. The higher prevalence and intensity of endoparasites helminth were found in swamp eel from natural caught than cultivation. Further studies were needed to molecular identified of endoparasite in swamp eel with a scanning electron microscope or 16rDNA.
The technology of striped catfish broodstock (Pangasius hypophthalmus) in high-quality maintenance
Authors: Wardani A I , Sari L A , Sari P D W , Nindarwi D D , Arsad S 
Published: April 2021

AbstractStriped catfish(P. hypophthalmus)is a high economic value and is a determining factor at the hatchery stage. Quality catfish brooders that can produce superior seeds can be obtained through good handling. The purpose of this study is to gain knowledge in the maintenance techniques of catfish broodstock. The working methods used in the study is a descriptive method with primary and secondary data collection. Striped catfish broodstock uses a recirculation system which includes preparation of pond maintenance, broodstock maintenance, feed management, water quality management, broodstock selection, and spawning. The results of the average water quality parameters for 25 days are turbidity 17.85 cm, temperature 29.51°C, DO 4.23 mg/L, ammonium 0.005 mg/L, and pH 7.54. The average weight measurements in the first and second sampling are 3.11 kg and 3.13 kg, while the average length measurements in the first and second sampling are 55 cm and 56.05 cm. The growth rate in the maintenance of catfish broodstock for 21 days is 9.52 grams/day, and the specific growth rate is 0.028%. Catfish spawning uses a ratio of ten males and three females.
The effect of feed management technology (life and pellet feed) on the maintenance of mutiara catfish (Clarias sp.) in freshwater cultivation
Authors: Dewanggani A P , Sari L A , Sari P D W , Nindarwi D D , Arsad S 
Published: April 2021

AbstractCatfish is currently the flagship commodity to increase economic value in Indonesia. Catfish farming is very profitable because good broodstock maintenance will produce quality tillers. A good catfish broodstock should have a feed management system, thereby improving the quality of mains. The purpose is to learn about feed management technology (life and pellet feed) on the maintenance of Mutiara catfish (Clariassp.). The method is with a descriptive method with immediate data retrieval and secondary data. Life feed is observed by taking water samples and then observed using a microscope. Life feed that is found using a microscope isSpirulinasp. andChlorellasp. Feed management on the maintenance of the broodstock of the Mutiara catfish (Clariassp.) is by feeding one time a day at a dose of 1.5 kg, the feed used is pellets PL 1 size 2 mm which has 33% protein and 5% fat, FCR which can be 2.43, absolute growth 15.42 kg, SGR 0.147 %, GR 2.4 gram/day, and SR 100%. Fish enlargement is a business activity maintenance fish up to the size of consumption. The feed is the largest component on the business of catfish enlargement.
The relationship with N/P ratio to phytoplankton abundance in mangrove Wonorejo waters, Rungkut, Surabaya, East Java
Authors: Arofah S , Sari L A , Kusdarwati R 
Published: April 2021

AbstractThe abundance of phytoplankton in water depends on the availability of nutrients. Nitrogen and phosphorus are two parameters that affect water. Phytoplankton require the elements of nitrate and phosphate in the production of fats and proteins. Nitrate and phosphate in water can be influenced by household waste discharges originating from residential areas around mangroves. The Wonorejo mangrove waters area is the estuary of an industrial waste journey. This study was conducted to determine the relationship of the N/P ratio to the abundance of phytoplankton in the Mangrove Waters of Wonorejo, Rungkut, Surabaya, East Java. Sampling was done at three stations with two replications. Identification or observation using a microscope. The results found that the most dominant class is the Bacillariophyceae consisted of two genus. Whereas the other classes found consisted of two genus Dynophyceae, one Clorophyceae, Coscinodiscophyceae, Fragilariophyceae and Cynophyceae. The results of the regression-correlation analysis showed a correlation coefficient of 0.885, meaning that the relationship obtained with an N / P ratio with an abundance of phytoplankton was very strong. The abundance of phytoplankton is influenced by the concentration of nitrogen and phosphate at 78.3% while the other 21.7% is influenced by physical and chemical factors of other waters.
The impact of COVID-19 on Indonesian fisheries conditions: opinion of current status and recommendations
Authors: Wiradana P A , Widhiantara I G , Pradisty N A , Mukti A T 
Published: April 2021

Abstract
The COVID-19 outbreak has spread rapidly across the world and has affected health, social, and economic impacts in many countries, including Indonesia. This editorial focuses on the implications of the COVID-19 pandemic on the fisheries sector in Indonesia along with recommendations on how we should deal with the current status. The pandemic has resulted in several negative impacts on the Indonesian community that is directly related to a significant decline in the demand for fisheries products. A range of problems has been reported, including marketing disturbances, issues related to post-harvest and cultivation cycles, and the livelihoods of small-scale fishermen. To overcome these issues, we provide recommendations for various stakeholders to rapidly mobilize the fisheries sector by assisting MSMEs and by helping to market fishery products online, thus facilitating the export of fishery products. These recommendations are expected to stimulate the fisheries sector to mitigate the long-term effects of the COVID-19 outbreak.
Prevalence and intensity of endoparasites in cutlassfish (Trichiurus sp.) at fish auction landing Panarukan Situbondo District and Muncar Banyuwangi District, East Java Province
Authors: Agustin S I , Subekti S , Ulkhaq M F , Amiin M K 
Published: April 2021

Abstract
Cutlassfish (Trichiurus sp.) is a kind of marine fishes has become an important economic commodity from several countries in Asia. The research was done to analyze the prevalence and intensity of endoparasite that infect cutlassfish (Trichiurus sp.), and to compare the prevalence and intensity of them at fish auction landing in Panarukan Situbondo and Muncar Banyuwangi, East Java Province. The method used in this research was an observation. The total of samples taken was 162 fishes. The average size of the examined cutlassfish is 56.47 ± 12.80 cm. The results showed the endoparasite that infects cutlassfish were Anisakis simplex and Camallanus carangis. The prevalence rate of endoparasites at fish auction landing respectively in Panarukan and Muncar were 23.46% and 13.58%, both of them were in the same category, often. The value of the intensity of endoparasite worms respectively in Panarukan and Muncar were 1.53 and 1.1 and both of them are also in the same category, low. Based on the Chi-square test stated that there was no significant difference (p>0.05) in the prevalence and intensity of endoparasite in both places.
Distribution of water turbidity concentrations and phosphate content in tanah merah beach and semujur island waters, central bangka regency
Authors: Yusuf M , Hudatwi M , Adi W , Robin  
Published: April 2021

Abstract
A large number of tin (Sn) mining activities carried out in the coastal area of Bangka Belitung has a direct impact on water quality, especially water turbidity. Turbid waters can produce large amounts of phosphate in local waters, and its distribution is influenced by the direction and speed of ocean currents. This study aims to determine the value of water turbidity concerning the amount of phosphate content in two different locations with tin (Sn) mining activities as a source of pollution. This research was conducted in July 2018, in the coastal waters of Tanah Merah Island and Semujur Island. The results of the water turbidity value in the Tanah Merah coastal waters were 12.50-22.50 NTU greater than that of Semujur Island waters, namely 4.14-7.22 NTU. The phosphate value in Tanah Merah coastal waters is 0.02-0.05 mg / L, greater than the Semujur Island waters, which is 0.01-0.02 mg / L. This indicates that Tanah Merah coastal waters are more turbid with a value higher phosphate. The existence of tin (Sn) mining activities in Tanah Merah waters has an impact on the amount of water turbidity that can bind phosphate elements compared to Semujur waters.
Experimental infection of Streptocccus agalactiae in silver rasbora (Rasbora argyrotaenia): Effect to hematological profile from infected fish
Authors: Nugrahani W A , Kusdarwati R , Ulkhaq M F 
Published: April 2021

Abstract
Silver rasbora (Rasbora argyrotaenia) is one of Indonesian local freshwater fishes species that have high economic value. One of bacterial diseases that probably infected to silver rasbora cultivation is Streptococcus agalactiae that caused streptococcosis. There was limited information about effect of Streptococcus agalactiae infection to hematological profile in silver rasbora has never been reported before. The aims of these studies were to determine the hematological profile of silver rasbora that experimentally infection with Streptococcus agalactiae. A total of four hundreds silver rasbora (6 ± 0.1 cm length and 3.6 ± 0.2 grams weight) were intramuscularly infection with 0,1 mL Streptococcus agalactiae in different density, include 104, 106, 108, 1010 and NaCl physiological solution as negative control. Observation of hematological profile was carried out by blood examination post infection which included total erythrocytes, total leukocytes, differential leukocytes (percentage of lymphocyte, neutrophil, and monocyte) and hemoglobin levels. Hematological profile in silver rasbora (Rasbora argyrotaenia) infected by Streptococcus agalactiae showed an increase in neutrophil and monocyte percentage and showed a decrease in total erythrocytes, hemoglobin, total leukocytes and lymphocyte percentage. Further studies were needed to prevent the Streptococcus agalactiae infection using herbal medicine.
Increased Immune Response of Carp (Cyprinus carpio L) by Giving Garlic (A. sativum) powder extract
Authors: Isroni W , Bahri A S , Maulida N 
Published: April 2021

Abstract
Cultivation by utilizing the stocking density and feed used is a driving force for the emergence of diseases due to decreased water quality due to the deposition of organic material from leftover food or fish excretion. Meanwhile, fish become stressed so they are vulnerable to disease attacks, especially infectious diseases such as those caused by bacteria or viruses. One of the natural ingredients that can enhance non-specific immune responses and fish growth is garlic powder. garlic is one of the natural plants which contains active ingredients such as sulfur compounds: alliin, allicin, disulfide, trisulfide; Enzymes such as Alinase, perinase; amino acids like arginine, and minerals like selenium. Allicin is one of the active substances that can kill pathogens (antibacterial) such as Aeromonas bacteria. While alliin contained in the white bottom can significantly improve the fish’s immune system so that garlic can be used as an efficient immunostimulant. The study was conducted using the experimental method. The addition of garlic in this study was: treatment A (0 g/kg of feed), treatment B (5 g/kg of feed), treatment C (10 g/kg of feed), treatment D (15 g/kg of feed), Treatment E (20 g/kg of feed) and Treatment F (25 g/kg of feed). The design used was a completely randomized design (CRD) with 6 treatments and 3 replications. The main parameters observed were the increase in non-specific immune responses including total leukocytes and phagocytic activity and added with absolute growth parameters. As supporting data water quality measurements are also carried out.
Study of the weather parameters effect on the maduris salt production
Authors: Mahabror D 
Published: April 2021

Abstract
The most widely used salt production technique is the process of evaporating seawater into salt by utilizing sunlight. This study conducted to measure the weather parameter and salt production during the salt season in 2019. The method used to measure the rate of evaporation was with a impermeable pan to prevent leakage of water samples. The results show that the evaporation rate of freshwater (0°Be) is greater than saltwater (20°Be) and the evaporation in July (0.62 cm/day) is smaller than October (1 cm/day). The correlation coefficient during July until October 2019 for air temperature (r) = 0.964, humidity (r) = -0.985, wind speed (r) = 0.622, and sunshine duration (r) = 0.987. The total salt production was from July until October 2019 reached 37395 kg on a demonstration plot area of 0.43 hectares. It is estimated the total of production per hectare will reach 87 tons /season. And for 2020 based on in-situ data it is estimated that production will decrease which is salt production will begin in July 2020, unlike 2019 which began in June, this is due to the influence of changes from the West wind (Asian monsoon) to East wind (Australian monsoon).
The potency of Pediococcus pentosaceus incubated with Moringa oleifera in fermentation process to increase nutrient content of rice bran
Authors: Lokapirnasari W P , Maslachah L , Sahidu A M , Yulianto A B 
Published: April 2021

AbstractThis study aimed to improve the nutritional quality of rice bran by usingPediococcus pentosaceusincubated withMoringa oleiferaextract (MOE) extract in rice bran fermentation. The treatments were divided into 7 treatments and 3 replications, as follows: P0: control; P1: 1%P. pentosaceus); P2: (1%P. pentosaceus+ 1%MOE); P3: (1%P. pentosaceus+ 2%MOE); P4: (2%P. pentosaceus; P5: (2%P. pentosaceus+ 1%MOE); P6: (2%P. pentosaceus+ 2%MOE).MOEwas added to probiotics according to the dose of treatment and then incubated at 37ºC for 24 hours. Furthermore, synbiotic had been used to ferment rice bran for 5 days under anaerobic conditions. The results indicate that there was a significantly different (p <0.05) in the group treatments and group control on the content of extract ether, crude protein, nitrogen-free extract, crude fiber, and metabolizable energy. The use of synbiotics in the treatment showed an increase in crude protein by 16.76%, a decrease in crude fiber by 23.35, a decrease in extract ether by 19.86%, NFE 9.47%, and an increase in metabolizable energy from 3057.41 to 3153.08 kcal/kg. Based on the results of this study, it could be concluded that the use ofP. pentosaceusincubated withM. oleiferaextract could be used to improve the nutritional quality of rice bran.
Hatching and harvesting techniques for Artemia cysts with different effects of salinity in the district of Situbondo, East Java
Authors: Bahr A S , Isroni W , Maulida N 
Published: April 2021

Abstract

Artemia sp. is a natural food that is very important in the hatchery of marine fish, crustaceans, freshwater fish consumption and ornamental fish. This happens because Artemia has a high nutritional value and the size that matches the mouth opening of almost all types of fish larvae. Artemia can be applied in a variety of fish and shrimp hatcheries. Good seawater, brackish and fresh. The advantage of Artemia compared to other natural foods in aquaculture is that they are traded in the form of cysts, so they can be used at any time if needed. The specialty of Artemia sp. as plankton is to have tolerance (the ability to adapt and defend themselves) in a very wide range of salt levels. At very high salt levels where no organism can survive, it turns out that Artemia sp. can tolerate it. The method used is an experimental method with the experimental design used is a Completely Randomized Design (CRD) with 3 replications and 5 treatments namely 15 ppt, 45 ppt, 60 ppt, 75 ppt 90 ppt and 30 ppt as a control. While the supporting parameters are water quality in culture media which includes temperature, pH, and oxygen.
A sustainable aquaculture model in Indonesia: multi-biotechnical approach in Clarias farming
Authors: Gustiano R , Prakoso V A , Radona D , S Dewi R R S P , Saputra A , Nurhidayat  
Published: April 2021

Abstract

Clarias catfish is the second rank of Indonesian commodity after tilapia in the production of freshwater aquaculture. It is due to the biotechnical innovations in aquaculture for breeding and grow-out, as well as selective breeding of superior fish. Nowadays, Clarias farming is a promising business because of a large market available in line with food security, fish farmers’ welfare, and sustainable aquaculture. The objectives of this paper are to review the latest status of Clarias catfish, improvement technologies used, challenges, constraints, and strategies to increase and maintain national production. In terms of seedlings production, the existing technology is relatively well-established with available segmentation in fingerling production. As for grow-out, many innovations such as the use of probiotics, biofloc, aquaponics, RAS, and IMTA provide opportunities for various business scales. The availability of technology based on a biotechnical approach enables to improve and maintain the sustainability of national Clarias production. The progress of Clarias farming in the last decade should be suggested as a model for other potential species. It is recommended that genetically improved Clarias by utilizing the potential of local catfish should be applied to produce specific advantages for development in certain areas and to increase business productivity.
Phagocytes response to dietary probiotics SEAL and Aeromonas hydrophila infection in red tilapia (Oreochromis sp.)
Authors: Purwanto A K R , Larasati B S , Istiqomah I , Isnansetyo A 
Published: April 2021

Abstract
This study focusses on the effect of the probiotic application on the phagocytosis activity, phagocytic index, and respiratory burst in red tilapia nilasa strain infected with Aeromonas
hydrophila. Yeast and bacterial strains of different origins (Sacharomyces sp. FNCC3015, Enterobacter sp. JC10, Aeromonas sp. JC33, and Lactococcus sp. JAL12) were administered to fish in daily, and three-day intervals at a dose of 5x104 CFU/gram feed. The control groups were only administered with fish pellets with saline buffer. Fish were infected with A. hydrophila in the LD70 dose on 23rd day of treatment. Phagocytic activity of blood leukocytes and blood respiratory burst were examined at one and two months of probiotic application, and 7 days after pathogenic bacteria infection. Administration of yeast and bacterial strains to fish before A. hydrophila infection elevated and prolonged phagocytic activity of blood leukocytes at the first and second months of application. No significant improvement of the phagocytic index and blood respiratory burst was found in the present study. It is therefore concluded that probiotics application at three-day interval is sufficient to stimulate and enhance the phagocytes performance of red tilapia nilasa strain before and after A. hydrophila infection.
Complement system activation in red tilapia (Oreochromis sp.) orally administered with probiotics SEAL
Authors: Dhanarso P , Yunissa H , Istiqomah I , Isnansetyo A 
Published: April 2021

Abstract
The purposes of this study were to determine the effect of probiotic application on the non-specific humoral immune system in red tilapia infected with Aeromonas hydrophila in a laboratory and field trial. Yeast and bacterial strains of different origin (Sacharomyces sp., Enterobacter sp. JC10, Aeromonas sp. JC33, and Lactococcus sp. JAL12) were administered to fish in daily and three day intervals at a dose of 5x104 cfu/gram feed. Fish were infected with A. hydrophila in the LD70 dose on 23rd day of treatment. All parameters were examined at one and two months of probiotic application, and 7 days of pathogenic bacteria infection. The results showed that dietary probiotics enhanced and prolonged serum antibacterial activity and total plasma protein. All probiotic application intervals induced higher bactericidal complement activity than the controls. Data on total plasma protein also indicated an increased in the humoral factor of probiotics treated fish. In the case of A. hydrohila infection, only the daily treatment of probiotics was able to keep the complement bactericidal activity of higher than the control. Field application of the probiotics required the probiotic doses of at least 105 cfu/gram feed to obtained an improve complement bactericidal activity.