bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel hydrocarbon-utilizing soil mycobacteria synthesize unique mycocerosic acids at a Sicilian everlasting fire
Authors: Smit Nadine T. , Villanueva Laura , Rush Darci , Grassa Fausto , Witkowski Caitlyn R. , Holzheimer Mira , Minnaard Adriaan J. , Sinninghe Damsté Jaap S. , Schouten Stefan 
Published: March 2021

Abstract. Soil bacteria rank among the most diverse groups of organisms on
Earth and actively impact global processes of carbon cycling, especially in
the emission of greenhouse gases like methane, CO2 and higher gaseous hydrocarbons. An abundant group of soil bacteria are the mycobacteria, which
colonize various terrestrial, marine and anthropogenic environments due to
their impermeable cell envelope that contains remarkable lipids. These
bacteria have been found to be highly abundant at petroleum and gas seep
areas, where they might utilize the released hydrocarbons. However, the
function and the lipid biomarker inventory of these soil mycobacteria are
poorly studied. Here, soils from the Fuoco di Censo seep, an everlasting
fire (gas seep) in Sicily, Italy, were investigated for the presence of
mycobacteria via 16S rRNA gene sequencing and fatty acid profiling. The
soils contained high relative abundances (up to 34 % of reads assigned)
of mycobacteria, phylogenetically close to the Mycobacterium simiae complex and more distant from the well-studied M. tuberculosis and hydrocarbon-utilizing M. paraffinicum. The soils showed decreasing
abundances of mycocerosic acids (MAs), fatty acids unique for mycobacteria,
with increasing distance from the seep. The major MAs at this seep were
tentatively identified as 2,4,6,8-tetramethyl tetracosanoic acid and
2,4,6,8,10-pentamethyl hexacosanoic acid. Unusual MAs with mid-chain methyl
branches at positions C-12 and C-16 (i.e., 2,12-dimethyl eicosanoic acid and
2,4,6,8,16-pentamethyl tetracosanoic acid) were also present. The molecular
structures of the Fuoco di Censo MAs are different from those of the
well-studied mycobacteria like M. tuberculosis or M. bovis and have relatively δ13C-depleted values (−38 ‰ to −48 ‰), suggesting a direct or indirect utilization of the released seep gases like methane or
ethane. The structurally unique MAs in combination with their depleted
δ13C values identified at the Fuoco di Censo seep offer a new
tool to study the role of soil mycobacteria as hydrocarbon gas consumers in
the carbon cycle.
Utilizing physics-based input features within a machine learning model to predict wind speed forecasting error
Authors: Vassallo Daniel , Krishnamurthy Raghavendra , Fernando Harindra J. S. 
Published: March 2021

Abstract. Machine learning is quickly becoming a commonly used technique for wind speed and power forecasting. Many machine learning methods utilize exogenous variables as input features, but there remains the question of which atmospheric variables are most beneficial for forecasting, especially in handling non-linearities that lead to forecasting error. This question is addressed via creation of a hybrid model that utilizes an autoregressive integrated moving-average (ARIMA) model to make an initial wind speed forecast followed by a random forest model that attempts to predict the ARIMA forecasting error using knowledge of exogenous atmospheric variables.
Variables conveying information about atmospheric stability and turbulence as well as inertial forcing are found to be useful in dealing with non-linear error prediction. Streamwise wind speed, time of day, turbulence intensity, turbulent heat flux, vertical velocity, and wind direction are found to be particularly useful when used in unison for hourly and 3 h timescales. The prediction accuracy of the developed ARIMA–random forest hybrid model is compared to that of the persistence and bias-corrected ARIMA models. The ARIMA–random forest model is shown to improve upon the latter commonly employed modeling methods, reducing hourly forecasting error by up to 5 % below that of the bias-corrected ARIMA model and achieving an R2 value of 0.84 with true wind speed.
AS PARTES E O TODO: A INSUFICIÊNCIA DOS DISCURSOS PRÉ-SOCRÁTICOS NO BANQUETE DE PLATÃO
Authors: Da Paz Andre , Pais Nunes Lopes Rodolfo 
Published: March 2021

Neste artigo analisaremos a analogia do uso dos nomes erōs-poiēsis realizada pela personagem Diotima em seu diálogo com o jovem Sócrates (205a-206a) no Banquete de Platão. Nesta analogia, Diotima analisa o uso do nome erōs tanto enquanto uma espécie particular de ‘desejo’ quanto enquanto o gênero geral que envolve todas as espécies particulares de ‘desejo’. Nosso estudo tem por objetivo compreender as consequências de lermos essa passagem em relação aos discursos dos cinco primeiro simposiastas, de modo a considerá-los como ao mesmo tempo discursos sobre aquilo que é e não é, ou seja, discursos simultaneamente verdadeiros, por falarem sobre características de Erōs no âmbito da espécie particular, e falsos, por tomarem tal parte (espécie) pelo todo (gênero). Neste momento do diálogo (205a-206a) haveria uma guinada no nível discursivo levada a efeito pela personagem Diotima, cuja preocupação metodológica em visar o mais geral leva-a a conduzir o jovem Sócrates em direção de formulações cada vez mais gerais no discurso, em detrimento às formulações particulares realizadas até então pelos demais simposiastas. Esses discursos levaram a efeito opiniões contraditórias não fundamentadas por um discurso adequado, sendo, portanto, aspectos particulares e insuficientes à apreensão do objeto buscado pela atividade dialética. Para ilustrar essa possibilidade de leitura, buscaremos evidenciar como os momentos na fala de Diotima posteriores à analogia erōs-poiēsis poderiam ser lidos como a identificação das insuficiências dos discursos precedentes, de modo a negá-los por meio de suprassunções, nomeadamente, negações que metodologicamente mantêm elementos a serem ressignificado num discurso mais generalizante, cuja finalidade seria a verdade.

PALAVRAS-CHAVE: Diotima, discursos, dialética, Banquete
Giften en woonplaats, een Belgische federale wetswijziging
Authors: van de Ven K.M.L.L. 
Published: March 2021

Giften en woonplaats, een Belgische federale wetswijziging
In deze bijdrage wordt ingegaan op de Belgische federale wetswijziging van 15 december 2020 die ervoor moet zorgen dat in meer gevallen dan voorheen in België over zogenoemde roerende schenkingen schenkbelasting wordt voldaan. Door de in beginsel verplichte registratie van roerende schenkingen in België wordt de invulling van het fiscale woonplaatsbeginsel meer van belang.
Mandatory family protection
Authors: Beuker M.R. 
Published: March 2021

Mandatory family protection
In deze boekbespreking analyseert de auteur het boek Mandatory family protection. Dit derde boek in een serie over rechtsvergelijkend erfrechtelijk onderzoek handelt over de dwingende bescherming die verschillende landen toekennen aan familieleden van erflaters. Diverse auteurs beschrijven de nationale regelingen van Europese landen, landen met een common law-stelsel, China en islamitische landen. Daarnaast is een aantal hoofdstukken gewijd aan historische en rechtsvergelijkende aspecten van dwingende erfrechtelijke bescherming. Duidelijk wordt dat het erfrecht constant in beweging is; stelsels met een legitieme portie zoeken flexibiliteit, terwijl rechtszekerheid gezocht wordt door stelsels met aanspraken gebaseerd op behoeften van familieleden.
Perfil de recém-nascidos de risco relacionado à termorregulação em Unidade de Terapia Intensiva Neonatal
Authors: Rodrigues Guimarães de Aquino Alana , Coeli Oliveira da Silva Bárbara , Barreto Vanessa Pinheiro , Rodrigues Guimarães de Aquino Alyne , Vasconcelos Trigueiro Elizabeth , Rodrigues Feijão Alexsandra 
Published: March 2021

Objetivo: Analizar el patrón de temperatura de recién nacidos de bajo peso al nacer ingresados en una Unidad de Cuidados Intensivos Neonatales.Metodología: Este es un estudio transversal y cuantitativo realizado en una Unidad de Cuidados Intensivos Neonatales en un hospital de maternidad en el noreste de Brasil. La muestra consistió en 45 niños de bajo peso al nacer, muy bajo peso al nacer o extremadamente bajo peso al nacer ingresados en la unidad.Resultados: Al ingreso se obtuvo una temperatura axilar promedio de 34.98ºC con una desviación estándar de 1.12. La tasa de hipotermia al ingreso fue considerablemente severa, por lo que en la primera hora, con seis horas y con 12 horas de hospitalización, el porcentaje de recién nacidos hipotérmicos (<36.5ºC) fue respectivamente: 93.33%, 73.33 % y 57.78%.Conclusión: Hubo fallas en los procesos cuando se trata de termorregulación, dado que casi toda la muestra llega con potencial estrés por frío.
Objective: To analyze the temperature pattern of low birth weight newborns admitted to a Neonatal Intensive Care Unit Methodology: This is a cross-sectional and quantitative study conducted in a Neonatal Intensive Care Unit of a maternity hospital in Northeastern Brazil. The sample consisted of 45 low birth weight, very low birth weight or extremely low birth weight newborns admitted to the unit.Results: At admission, we obtained an axillary temperature average of 34.98ºC with a standard deviation of 1.12. The hypothermia rate at the time of admission was considerably severe, so that in the first hour, with 06 hours and with 12 hours of hospitalization, the percentages of hypothermic newborns (<36.5ºC) were respectively: 93.33%, 73.33 % and 57.78%.Conclusion: We found flaws in the processes when it comes to thermoregulation, given that almost the entire sample arrives with potential cold stress.
Objetivo: Analisar o padrão de temperatura dos recém-nascidos de baixo peso internados em uma Unidade de Terapia Intensiva Neonatal.Metodologia: Trata-se de um estudo transversal e quantitativo realizado numa Unidade de Terapia Intensiva Neonatal de uma maternidade no Nordeste do Brasil. A amostra foi constituída de 45 recém-nascidos de baixo peso, muito baixo peso ou extremo baixo peso admitidos na unidade. Resultados: Na admissão obteve-se uma média de temperatura axilar de 34,98ºC com desvio padrão de 1,12. A taxa de hipotermia na admissão foi consideravelmente grave, de forma que na primeira hora, com seis horas e com 12 horas de internação a porcentagem de recém-nascidos hipotérmicos (<36,5ºC) foi respectivamente: 93,33%, 73,33% e 57,78%. Conclusão: Verificou-se falhas nos processos quando se trata de termorregulação, haja vista que quase a totalidade da amostra chega com potencial estresse ao frio.
La concentración de fluoruro en las aguas consumidas en la Región de Murcia no es suficiente para prevenir la caries dental
Authors: Pérez Silva Amparo , Aparecido Cury Jaime , Serna Muñoz Clara , Cabello Malagon Inmaculada , Martínez Beneyto Yolanda , Ortiz Ruiz Antonio José 
Published: March 2021

Introducción: La caries sigue siendo la enfermedad crónica más frecuente en el niño y es considerada un problema de salud pública. El flúor es la principal medida protectora contra la caries dental y el agua de consumo es la principal fuente de ingesta de flúor.Objetivo: El objetivo de nuestro trabajo fue calcular la concentración de ión fluoruro en el agua de abastecimiento de 10 zonas y en 10 aguas embotelladas comercializadas en la Región de Murcia.Material y Métodos: La concentración de fluoruro en las aguas se determinó con un electrodo ion–selectivo para flúor previamente calibrado con patrones de fluoruro preparados con TISAB II.Resultados: En el agua de abastecimiento, se detectaron concentraciones de fluoruro entre 0.09 y0.18 mgF/L (ppm) en las aguas embotelladas la concentración de fluoruro varió desde 0.04 a 0.50 ppm.Conclusiones: El fluoruro está presente en aguas consumidas en la Región de Murcia pero enconcentraciones que no alcanzan niveles preventivos para la caries dental. Es necesario prescribir suplementos de flúor en niños con alto riesgo de caries y para ello se deben contabilizar todas las fuentes externas de flúor, incluido el agua.
Introduction: Dental caries remains the most frequent chronic disease in childhood and is considered a public health problem. Fluoride has proven effectiveness in the prevention of caries and drinking water is the main source of fluoride intake. The objective of this study was to determine fluoride concentrations in tap water from 10 areas and in 10 bottled waters sold in the Region of Murcia.Materials and Methods: The concentration of fluoride in water was determined using a fluoride ion-selective electrode with a direct technique previously calibrated with standard fluoride concentrations prepared with TISAB II.Results: In tap water, fluoride concentrations from 0.09 to 0.18 mgF/L (ppm) were detected; in bottled waters the concentration of fluoride varied from 0.04 to 0.50 ppm.Conclusion: Fluoride is present in the water consumed in the Region of Murcia but in concentrations that do not prevent dental caries. Fluoride supplements should be prescribed in children at high risk of caries, and for this, all external sources of fluoride intake, including water, must be accounted for.
Influence de la consommation de substances psychoactives sur le fonctionnement adaptatif des étudiants du postsecondaire présentant un TDAH
Authors: Lagacé Leblanc Jeanne , Massé Line , Plourde Chantal 
Published: March 2021

Objectifs. Cette étude vise à étudier l’association entre la consommation de substances psychoactives (SPA) et le fonctionnement adaptatif des étudiants présentant un trouble de déficit de l’attention/hyperactivité (TDAH) à l’éducation postsecondaire. Les atteintes fonctionnelles liées au TDAH et les types de SPA privilégiée par cette population sont également examinés.
Méthode. L’échantillon est constitué de 243 étudiants de niveau collégial (56,7 %) et universitaire (43,6 %) de la province de Québec présentant un TDAH et âgés en moyenne de 24 ans.
Résultats. Une plus grande proportion d’étudiants universitaires présentent une consommation à risque d’alcool comparativement aux collégiens. Le cannabis et les speeds se révèlent comme les substances de choix pour ceux qui consomment des drogues (43,3 %). De plus, les analyses de la covariance multivariées montrent un effet principal significatif de la sévérité de la consommation d’alcool selon les domaines fonctionnels. La sévérité de la consommation d’alcool et de drogues est associée à la manifestation d’autres comportements à risque.
Conclusion. La consommation de SPA influence de façon importante la vie des étudiants ayant un TDAH.
Habitudes de consommation de substances psychoactives chez les adolescents et jeunes adultes autistes
Authors: Poulin Marie-Hélène , Laventure Myriam , Tremblay Kelly , Beuchot Emma 
Published: March 2021

La légalisation du cannabis étant récente au Canada, les intervenants en autisme sont préoccupés par la consommation des adultes autistes qui sont plus à risque de développer une dépendance. L’automédication de l’anxiété et les difficultés d’inclusion sociale pourraient encourager la consommation de cannabis. Cette étude vise à décrire les habitudes de consommation de substances psychoactives (SPA ; tabac, alcool, cannabis, autres drogues) et leurs conséquences chez les personnes ayant un diagnostic de trouble de spectre de l’autisme (TSA) âgées de 16 à 30 ans. Une enquête en ligne par questionnaire a été complétée par 65 adolescents et adultes (âge moyen = 21,4 ans) ayant un TSA (32 hommes et 33 femmes). La consommation de SPA a été mesurée à l’aide du DEP-ADO version 3.3 (Germain et al., 2016) adaptée. Fait marquant, 36,9 % des personnes autistes (12 hommes et 12 femmes) rapportent n’avoir rien consommé (alcool, tabac et drogues) au cours des 12 derniers mois. Les personnes autistes qui consomment rapportent une consommation accrue de cannabis (quotidienne) et d’autres drogues. Pourtant, la gravité de leur consommation est comparable à leurs pairs non autistes selon les données disponibles dans la littérature. Par ailleurs, la prévalence de consommation problématique est très faible dans la population autiste (7,7 %). Les principales conséquences rapportées concernent la perte d’argent, les difficultés de santé et les conduites à risque. Les filles ayant un TSA mentionnent vivre davantage de conséquences quant aux conduites à risque liées à leur consommation de SPA. Des recommandations cliniques pour l’accompagnement des personnes autistes et de leurs proches ainsi que des pistes de recherches futures sont proposées.
Effets sociaux, culturels et psychologiques du mariage chez les adultes tamouls de seconde génération en France. Étude de cas – Renuka : un mariage arrangé à l’occidentale
Authors: Vijayaratnam Mélanie , Simon Amalini , Radjack Rahmeth , Moro Marie Rose 
Published: March 2021

Objectif Cette étude se veut une approche compréhensive des conflits psychiques que rencontrent les jeunes femmes confrontées à des modèles culturels divergents. Elle relate des tensions traversées par Renuka, Tamoule née en France, dans ses choix matrimoniaux entre verticalité culturelle et métissage.
Méthode Le parcours matrimonial de Renuka est exploré à travers des entretiens semi-directifs approfondis analysés selon la méthode complémentariste de Devereux.
Résultats Dans un espace pluriculturel, il y a une souffrance résultant de la difficulté de positionnement. Les choix maritaux révèlent des tensions intrapsychiques qui peuvent être dépassées en mobilisant des stratégies, des processus et des mécanismes articulant les modèles identificatoires.
Conclusion Cette étude explore les enjeux du mariage en contexte migratoire et ses effets sur la vie psychique des individus et relate une des problématiques majeures de nos sociétés métissées.
Phenotype study of multifoliolate leaf formation inTrifolium alexandrinumL.
Authors: Malaviya Devendra Ram , Roy Ajoy Kumar , Kaushal Pankaj , Pathak Shalini , Kalendar Ruslan 
Published: March 2021

BackgroundThe genusTrifoliumis characterized by typical trifoliolate leaves. Alterations in leaf formats from trifoliolate to multifoliolate, i.e., individual plants bearing trifoliolate, quadrifoliolate, pentafoliolate or more leaflets, were previously reported among many species of the genus. The study is an attempt to develop pure pentafoliolate plants ofT. alexandrinumand to understand its genetic control.MethodsThe experimental material consisted of two populations ofT. alexandrinumwith multifoliolate leaf expression, i.e.,interspecific hybrid progenies ofT. alexandrinumwithT. apertum, andT. alexandrinumgenotype Penta-1. Penetrance of the multifoliolate trait was observed among multifoliolate and trifoliolate plant progenies. In vitro culture and regeneration of plantlets from the axillary buds from different plant sources was also attempted.ResultsThe inheritance among a large number of plant progenies together with in vitro micro-propagation results did not establish a definite pattern. The multifoliolate leaf formation was of chimeric nature, i.e., more than one leaf format appearing on individual branches. Reversal to normal trifoliolate from multifoliolate was also quite common. Penetrance and expression of multifoliolate leaf formation was higher among the plants raised from multifoliolate plants. Multifoliolate and pure pentafoliolate plants were observed in the progenies of pure trifoliolate plants and vice-versa. There was an apparent increase in the pentafoliolate leaf formation frequency over the years due to targeted selection. A few progenies of the complete pentafoliolate plants in the first year were true breeding in the second year. Frequency of plantlets with multifoliolate leaf formation was also higher in in vitro axillary bud multiplication when the explant bud was excised from the multifoliolate leaf node.ConclusionNumber of leaflets being a discrete variable, occurrence of multifoliolate leaves on individual branches, reversal of leaf formats on branches and developing true breeding pentafoliolates were the factors leading to a hypothesis beyond normal Mendelian inheritance. Transposable elements (TEs) involved in leaf development in combination with epigenetics were probably responsible for alterations in the expression of leaflet number. Putative TE’s movement owing to chromosomal rearrangements possibly resulted in homozygous pentafoliolate trait with evolutionary significance. The hypothesis provides a new insight into understanding the genetic control of this trait inT. alexandrinumand may also be useful in otherTrifoliumspecies where such observations are reported.
Genotoxic Effect of Arsenate and Arsenite in Human HaCat Cells in Culture Using Comet Assay
Authors: Benhusein Ghazalla , Mutch Elaine , Williams Faith M. 
Published: March 2021

Arsenic is an environmental chemical of toxicological concern today since it is a human genotoxin and chronic exposure is associated with development of cancers, including skin. Inorganic arsenate is metabolically reduced to arsenite by glutathione (GSH) prior to methylation. The aim of this study was to determine the relative toxic effects of arsenate and arsenite in HaCat cells (immortalized human keratinocytes) in vitro by measuring cytotoxicity, DNA damage, depletion of glutathione and apoptotic and necrotic events. HaCat cells were treated with arsenate and arsenite (10 μM) for DNA damage detection using Comet assay and cytotoxicity (10, 60 and 100 μM) all measured at 24 hr. In some experiment arsenate or arsenite (10 μM) was added at the same time as BSO 10 μM for 24 hr, and GSH levels were measured by HPLC with fluorescence detection. Flow cytometry was used to investigate apoptotic and necrotic events following arsenate and arsenite (10 μM) treatment for 24 hr. Arsenate and arsenite at 60 and 100 μM, but not 10 μM, reduced the number of adherent viable cells with time. Therefore, DNA damage could only be measured at 10 μM as at higher concentrations the cells did not produce classical Comets but showed fragmentation. DNA damage was significantly (p < 0.001) increased in cells treated for 24 hr with 10 μM arsenate and arsenite compared to control. GSH levels were significantly increased in HaCat cells treated with10 μM arsenate and arsenite (p < 0.05, p < 0.001, respectively) compared to control. Cells treated with buthionine sulphoximine (BSO) at the same time as arsenate had increased GSH levels (p < 0.001), but arsenite and BSO did not increase cellular GSH. Arsenate and arsenite increased apoptosis, and arsenate increased necrosis, although none of the values reached statistical significance. Arsenite was more cytotoxic than arsenate. Arsenate and arsenite are known to produce oxidative stress involving ROS formation and depletion of glutathione. The increase in GSH levels at low doses of arsenate and arsenite, and by arsenate even in the presence of BSO.