bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Antibiotic Resistance in Vibrio cholerae: A Meta-analysis
Authors: Bagheri-Josheghani Sareh , Bakhshi Bita , Mousavi Maryam 
Published: February 2021

Abstract
Background:Cholera could cause severe watery diarrhea and even death, but in patients diagnosed with severe dehydration, treatment with an appropriate antimicrobial agent could decrease the volume of diarrhea and shorten the duration of V. cholerae shedding. Unfortunately, due to the high antibiotic’s consumption, antibiotic resistance in V. cholerae strains is progressively increasing worldwide. The present systematic review and meta-analysis study aimed to investigate the incidence of antibiotic resistance in V. cholerae strains worldwide.Methods: The incidence of antibiotic resistance in V. cholerae strains was assessed by conducting a systematic review and searching international databases including PubMed and Google Scholar for articles published from Jan 1, 1990 to Sept 30, 2016 using related keywords. Studies were selected to be included in this systematic review according to a predefined eligibility criteria. International databases were searched for articles evaluating V. cholerae strains resistance rates to antibiotics. Pooled estimates of antibiotics resistance and 95% confidence intervals (CIs) were categorized based on WHO regions. Results:V. cholerae was the most common bacterium showing high resistance rates to various antibiotics including furazolidone (83%), trimethoprim-sulfamethoxazole (67%), nitrofurantoin (66%), streptomycin (64%), and nalidixic acid (58%).Conclusions: According to this meta-analysis results, the high resistance of V. cholerae strains to antibiotics could be considered as a global public health threat. Therefore, it is recommended to select appropriate antibiotic treatment regimens for each region based on antibiotic resistance patterns of local strains in that region.
Treatment Outcome With Fosravuconazole L-lysine Ethanolate (F-RVCZ) for Onychomycosis
Authors: Shimoyama Harunari , Yo Ayaka , Sei Yoshihiro , Kuwano Yoshihiro 
Published: February 2021

Abstract
Fosravuconazole L-lysine ethanolate (F-RVCZ), a ravuconazole prodrug, is a newly available agent with high expectations for efficacy in the treatment of onychomycosis. However, clinical data regarding the efficacy of F-RVCZ are limited because the drug was launched only in Japan in 2018. Therefore, we analyzed the outcome of F-RVCZ therapy in the treatment of onychomycosis at outpatient dermatology clinics in Japan. We examined data for 109 patients (68 male, 41 female) with varying clinical type, including total dystrophic onychomycosis and dermatophytoma, and a wide range of age groups, including the elderly. The complete cure rate at 12 weeks was 6.4% (7/109) and 67.9% (74/109) at the last visit (mean time to last visit: 32±14.2 weeks). Mean rate of improvement in the affected nail area was 49.1±23.3% at 12 weeks and 86.8±22.4% at the last visit. Efficacy at 12 weeks and the last visit, respectively, was as follows: none, 4 cases and 1 case; slight, 35 cases and 4 cases; moderate, 51 cases and 21 cases; significant, 12 cases and 9 cases; complete cure, 7 cases and 74 cases. There were no serious adverse events. This retrospective survey was the first large-scale analysis of actual clinical practice outcomes and had minimal exclusions. Compared to previous reports, our results demonstrated excellent efficacy of F-RVCZ therapy in a variety of patients. Considering our results and the ease of oral administration (1 capsule/day for 12 weeks) and few adverse events, F-RVCZ therapy appears to be a useful option for the treatment of onychomycosis.
Tribological interaction of&nbsp;plasma functionalized CaCO3 nanoparticles with zinc and ashless dithiophosphate additives
Authors: Vyavhare Kimaya P , Timmons Richard B. , Erdemir Ali , Aswath Pranesh B. 
Published: February 2021

Abstract
Surface-modified CaCO3 nanoparticles, synthesized through plasma-enhanced chemical vapor deposition (PECVD), were employed to improve lubricant additive technology for internal combustion engines via reduction and/or replacement of additives, such as zinc dialkyl dithiophosphate (ZDDP), in engine oil. Various oil formulations were prepared with functionalized CaCO3 nanoparticles, in combination with ashless dialkyl dithiophosphate (DDP) and ZDDP at low concentrations of phosphorus. Tribological test results indicate synergistic interaction of functionalized CaCO3 nanoparticles with ZDDP and DDP, providing enhanced friction and wear performance under boundary lubrication. A comparative study of the tribo-surfaces morphology and chemistry was assessed via atomic force microscopy and X-ray absorption near-edge spectroscopy. Improved wear protection by functionalized CaCO3BM (borate and methacrylate coated) nanoparticles under boundary lubrication was attributed to the formation of calcium and boron-rich 50–80 nm thick tribofilms on the worn surfaces. XANES results revealed that plasma functionalized CaCO3 nanoparticles interact with ZDDP and DDP and participate in tribofilm formation through tribo-chemical reactions and metal cation supply to form stable and wear-resistant tribofilms. These results provide strong support for the potential application of plasma functionalized CaCO3 nano-additives to reduce the concentration of harmful P-based additives in automotive lubricants.
Identification of a nine ferroptosis-related lncRNA prognostic signature for lung adenocarcinoma
Authors: Tong Xiwen , Zhang Yujiao , Yang Guodong , Yi Guanghui 
Published: February 2021

Abstract
Background

Recently, mounting of studies has shown that lncRNA affects tumor progression through the regulation of ferroptosis. The current study aims to construct a robust ferroptosis-related lncRNAs signature to increase the predicted value of lung adenocarcinoma (LUAD) by bioinformatics analysis.
Methods

The transcriptome data were abstracted from The Cancer Genome Atlas (TCGA). Differentially expressed lncRNAs were screened by comparing 535 LAUD tissues with 59 adjacent non-LAUD tissues. Univariate Cox regression, lasso regression, multivariate Cox regression were conducted to design a ferroptosis-related lncRNA signature. This signature’s prognosis was verified by the log-rank test of Kaplan-Meier curve and the area under curve (AUC) of receiver operating characteristic (ROC) in train set, test set, and entire set. Furthermore, univariate and multivariate Cox regression were used to analyze its independent prognostic ability. The relationship of the ferroptosis-linked lncRNAs' expression and clinical variables was demonstrated by Wilcoxon rank-sum test and Kruskal-Wallis test. Gene set enrichment analysis (GSEA) was performed to signaling pathways it may involve.
Results

1224 differentially expressed lncRNAs were idendified, of which 195 are ferroptosis-related lncRNAs. A nine ferroptosis-related lncRNAs (AC099850.3, NAALADL2-AS2, AL844908.1, AL365181.2, SMIM25, FAM83A-AS1, LINC01116, AL049836.1, C20orf197) prognostic signature was constucted. This model's prognosis in the high-risk group is obviously worse than that of the low-risk group in train set, test set, and entire set. The AUC of ROC predicting the three years survival in the train set, test set, and entire set was 0.754, 0.716, and 0.738, respectively. Moreover, the designed molecular signature was found to be an independent prognostic variable. The expression of these lncRNAs and the lncRNA signature are related to clinical stage, T stage, Lymph-node status, distant metastasis. Finally, GSEA analysis results show that the signature is involved in eight tumor-related and metabolism-related signaling pathways
Conclusion

The current study constructed, validated, and evaluated a nine ferroptosis-related lncRNA signature which can independently be used to predict the prognosis of LAUD patients, and may become a new therapeutic target.
Immediate and lasting impact of combining restrictive and enabling interventions to reduce aztreonam consumption in a community hospital
Authors: Mody Dviti , Burke Christopher , Minson Quentin 
Published: February 2021

Abstract
Background. Antimicrobial stewardship initiatives combining restrictive and enabling components may be an effective strategy to achieve short- and long-term objectives. Aztreonam, a relatively high-cost antipseudomonal antibiotic, is an appropriate target for stewardship initiatives based on propensity for overuse in penicillin allergy, an activity profile often warranting additional empiric gram-negative and gram-positive coverage, and a unique durability to Ambler class B metallo-beta-lactamases.Objective. Analyze the immediate and long-term impact on aztreonam prescribing of combining restrictive and enabling interventions.Setting. Single 233-bed community hospital with 45 adult intensive care unit beds in Nashville, Tennessee.Method. Retrospective, interrupted time series analysis comparing all patients receiving aztreonam prior to intervention between January 1, 2010 and September 30, 2011 and following intervention between October 1, 2011 and September 30, 2019. Quarterly defined daily doses/1000 adjusted patient days and microbiology laboratory annual surveillance data were utilized for analysis. Main outcome measure. Post-intervention change in trend of aztreonam consumption. Results. Following intervention, a significant decline in aztreonam consumption was observed (-1.97 defined daily doses/1000 adjusted patient days; p = 0.003) resulting in a sustained decrease in aztreonam consumption from 2011 (3rd quarter) to 2019 (3rd quarter) from 15.2 to 0.26 defined daily doses/1000 adjusted patient days. Short-term group 2 carbapenem consumption increased (p = 0.044). Pseudomonas aeruginosa susceptibility to aztreonam improved from 2011 to 2018 (72% vs. 84%; p = 0.0004) without deleterious effects to alternative antipseudomonal beta-lactams. Conclusion. Combining restrictive and enabling interventions had immediate and sustained impact on aztreonam consumption with Pseudomonas aeruginosa susceptibility improvement.Impacts on practice: ● A pharmacist-driven intervention combining restrictive and enabling strategies produced an immediate and sustained decrease in aztreonam prescribing at a community hospital.● Following the sustained reduction of aztreonam consumption, susceptibility rates for Pseudomonas aeruginosa improved without producing deleterious effects on alternative antipseudomonal beta-lactams.
Evaluation of Residual Stress of C Oriented AlN/Si&nbsp;(111) and Its Impact on Mushroom Shaped Piezoelectric Resonator&nbsp;
Authors: PANDEY AKHILESH , Dutta Shankar , Gupta Nidhi , Kaur Davinder , Raman R. 
Published: February 2021

Abstract
Aluminum nitride-based MEMS resonators are one of the interesting recent research topics for its tremendous potential in a wide variety of applications. This paper focuses on the detrimental effect of residual stress on the AlN based MEMS resonator design for acoustic applications. The residual stress in the sputtered c axis (<001>) preferred oriented AlN layers on Si (111) substrates are studied as a function of layer thickness. The films exhibited compressive residual stresses at different thickness values: -1050 MPa (700 nm), -500 MPa (900 nm), and -230 MPa (1200 nm). A mushroom-shaped AlN based piezoelectric MEMS resonator structure has been designed for the different AlN layer thicknesses. The effect of the residual stresses on the mode shapes, resonant frequencies, and quality factor (Q) of the resonator structures are studied. The resonant frequency of the structures are altered from 235 kHz, 280 kHz, and 344 kHz to 65 kHz, 75 kHz and 371 kHz due to the residual stress of -1050 MPa (thickness: 700 nm), -500 MPa (thickness: 900 nm) and -230 MPa (thickness: 1200 nm) respectively. At no residual stress, the quality factors of the resonator structures are 248, 227, 241 corresponding to the 700 nm, 900 nm, and 1200 nm thick AlN layers respectively. The presence of the residual stress reduced the Q values from 248 (thickness: 700 nm), 227 (thickness: 900 nm), 241 (thickness: 1200 nm) to 28, 53, and 261 respectively.
The effectiveness of non-pharmacological or non-surgical interventions on the Health Related Quality of Life of People with Parkinson's Disease: a protocol of systematic review of randomized controlled trials
Authors: Paula Tamara Cristine de , Alcântara-Silva Tereza Raquel de Melo , Citon Leonardo Francisco , Porto Celmo Celeno 
Published: February 2021

Abstract
Background: Parkinson's disease (PD) is the second most common neurodegenerative, progressive and disabling disease affecting the elderly population, with by motor and non-motor symptoms that interfere with the Health-Related Quality of Life (HRQoL) of this population. Currently, there are still no resources to cure the disease, so the main treatment method used to reduce symptoms is based initially on pharmacological therapy, and in some cases, surgery is recommended. However, some unconventional interventions have been used, showing good results for the improvement of symptoms that also include HRQoL. Therefore, this investigation aimed to identify non-pharmacological or non-surgical interventions, in randomized clinical studies (RCTs) and their effectiveness in improving the HRQoL of people with PD.Methods: Systematic review, guided by the PRISMA method, using the SciELO, MEDLINE / PubMed, Cochrane Library, Web of Science, Scopus, and Brazilian Digital Library of Theses and Dissertations databases - BDTD, through CAFe Access on the CAPES / MEC Journals portal. "Quality of Life" - "Parkinson’s Disease" - "Randomized Controlled Trial", were the keywords used. Articles were selected by 2 independent reviewers using a third for doubts / discrepancies. Articles published until October 2020, in languages English, Portuguese and Spanish, RCTs, with non-pharmacological or non-surgical interventions, population diagnosed with PD, at any age or stage of the disease will be included. A narrative synthesis will be used to summarize the results and discussion, as well as a methodological evaluation of the RCTs using Cochrane Collaboration's risk of bias tool.Discussion: This review will identify the effectiveness of non-pharmacological and non-surgical interventions aimed at improving the HRQoL of people with PD, based on assessment instruments related to the proposed theme, based on RCT. Therefore, the findings show the current panorama of studies performed, discussion about their clinical applicability, as well as suggestions for improvements of future trials.Registration of Systematic Review: CRD42021221383
The Predictive Value of CHA2DS2–VASc Score as a Predictor for Left Ventricular Thrombus After Acute Anterior ST-Elevation Myocardial Infarction: A Case-Control Retrospective Analysis
Authors: Zheng Nan , Zang Jun 
Published: February 2021

Abstract
Objective: To determine the predictive value of CHA2DS2–VASc score as a predictor for left ventricular thrombus after acute anterior ST-elevation myocardial infarction (STEMI).Methods: We performed a case–control retrospective study of 30 patients with left ventricular thrombus (median age: 60.6 years, range: 38–75 years old; 26 males, 74 females) and 60 age- and sex-matched controls without left ventricular thrombus. Correlation analysis was performed and receiver operating characteristic (ROC) curve was used to evaluate the predictive value of CHA2DS2–VASc score in detecting left ventricular thrombus.Result: Patients with left ventricular thrombus after acute anterior STEMI had higher CHA2DS2–VASc scores than controls (P < 0.001). Correlation analysis revealed that CHA2DS2–VASc scores were positively correlated with left ventricular thrombus after acute anterior STEMI (r = 0.413; P < 0.001). Multiple logistic regression analyses indicated that CHA2DS2–VASc scores (P = 0.001) and heart failure (P = 0.023) were risk factors of left ventricular thrombus. The ROC curve of CHA2DS2–VASc scores revealed that area under curve was 0.746 (95% confidence interval: 0.638–0.853, P < 0.001), sensibility was 86.7%, and specificity was 50.0%.Conclusion: Patients with heart failure have a high incidence of left ventricular thrombus after acute anterior STEMI. CHA2DS2–VASc scores contribute to the prediction for left ventricular thrombus after acute anterior STEMI.
Biodegradable and Transparent Films With Tunable UV-blocking Property From Xylose Residue by a Top-down Approach
Authors: Song Yijia , Chen Sheng , Chen Yanglei , Xu Yanglei , Xu Feng 
Published: February 2021

Abstract
As over exposure of the earth to ultraviolet (UV) light and increased amount of petroleum-based plastic waste, biodegradable UV-blocking materials are desired for diverse sustainable applications. Xylose residue, as the byproduct of xylitol production from corn cobs, is mainly composed of cellulose and lignin. Here, we develop a series of xylose residue films through a top-down approach (i.e., tunable delignification and regeneration) without any additional additives. The treated xylose residues with lignin content of 4.4-29.7% are used to prepare regenerated films, which exhibit excellent UV-blocking capability: 68.6-99.2% for UVB (290-320 nm) and 47.1-98.2% for UVA (320-400 nm). Moreover, these films remain a great optical transparency (50.6-86.6%) and show enhanced water vapor permeability (2.17-2.76 ×10-11 g·cm·cm-2·s-1·mmHg-1), surface hydrophobicity (water contact angle=72.3-86.4°), and thermal stability. Overall, our sustainable UV-blocking films have potential applications in the fields of electronics, food packaging, and windshields, etc. This study provides new insights into converting xylose residue directly to high value-added functional bioproducts.
Effects of Short-term Application of Moutai Lees Biochar on Nutrients and Fungal Community Structure in Yellow Soil of Guizhou
Authors: Zhang Meng , Liu Yanling , Wei Quanquan , Gou Jiulan 
Published: February 2021

Abstract
In order to realize the resource utilization of Guizhou sauce-flavor distiller's grains and the improvement of yellow soil fertility, a field experiment was carried out to study the effects of short-term application of vinasse biochar on soil nutrients and the diversity of fungal community structure by setting five biochar dosages of 0% (MB0), 0.5% (MB0.5), 1.0% (MB1.0), 2.0% (MB2.0), and 4.0% (MB4.0). The results showed that the application of lees biochar increased the pH, soil organic matter (SOM), total nitrogen (TN), ammonium N (AN), nitrate N (NN), available phosphorus and available potassium of the yellow soil to varying degrees, but decreased the microbial biomass carbon (MBC) and microbial biomass N (MBN) by 12.36%-26.49% and 34.10%-59.95% respectively. The application of lees biochar significantly reduced the number of fungal OTUs and community diversity. Compared with MB0 treatment, the application of lees biochar significantly changed the structure of the fungal community. The relative abundances of Mortierellomycota, Basidiomycota, Glomeromycota, and Chlorophyta were all increased in varying degrees, but the relative abundance of Ascomycota was significantly reduced by 23.86%-29.06%. At the same time, the application of lees biochar also increased the relative abundance of some functional bacteria, such as Mortierella and Chaetomium, and reduced the relative abundance of some pathogenic bacteria, such as Aspergillus and Fusarium. In addition, the results of redundant analysis showed that SOM, AN and NN were the main environmental factors that affect the change of yellow soil fungal community structure. In summary, the short-term application of lees biochar can increase the nutrient content of soil, change the structure and diversity of soil fungal communities, and also can reduce the relative abundance of some pathogenic bacteria, which can inhibit the growth and reproduction of harmful plant pathogens.
Optimization of whole-genome sequencing of&nbsp;Plasmodium falciparum&nbsp;from low-density dried blood spot samples
Authors: Teyssier Noam , Chen Anna , Duarte Elias , Sit Rene , Greenhouse Bryan , Tessema Sofonias 
Published: February 2021

Abstract
Background Whole-genome sequencing (WGS) is becoming increasingly useful to study the biology, epidemiology, and ecology of malaria parasites. Despite ease of sampling, DNA extracted from dried blood spots (DBS) has a high ratio of human DNA compared to parasite DNA, which poses a challenge for downstream genetic analyses. The effects of multiple methods for DNA extraction, digestion of methylated DNA, and amplification were evaluated on the quality and fidelity of WGS data recovered from DBS.MethodsLow parasite density mock DBS samples were created, extracted either with Tween-Chelex or QIAamp, treated with or without McrBC, and amplified with one of three different amplification techniques (two sWGA primer sets and one rWGA). Extraction conditions were evaluated on performance of sequencing depth, percentiles of coverage, and expected SNP concordance.ResultsAt 100 parasites/μL, Chelex-Tween-McrBC samples had higher coverage (5X depth = 93% genome) than QIAamp extracted samples (5X depth = 76% genome). The two evaluated sWGA primer sets showed minor differences in overall genome coverage and SNP concordance, with a newly proposed combination of 20 primers showing a modest improvement in coverage over those previously published.ConclusionsOverall, Tween-Chelex extracted samples that were treated with McrBC digestion and are amplified using 6A10AD sWGA conditions had minimal dropout rate, higher percentages of coverage at higher depth, and more accurate SNP concordance than QiaAMP extracted samples. These findings extend the results of previously reported methods, making whole genome sequencing accessible to a larger number of low density samples that are commonly encountered in cross-sectional surveys.
A high prevalence of NCD multimorbidity in South African Adolescents and Youth Living with HIV: Implications for Integrated Prevention
Authors: Kamkuemah Monika , Gausi Blessings , Oni Tolu 
Published: February 2021

Abstract
Background: Adolescents and youth living with HIV (AYLHIV) face an elevated NCD risk resulting from HIV, psychosocial challenges and the complications of antiretroviral therapy (ART). Methods: We conducted a cross-sectional study in six primary care facilities to investigate the prevalence of common NCDs and risk factors among AYLHIV in Cape Town, South Africa between March 2019 and January 2020. We collected socio-demographic information, ascertained behaviours and knowledge and screened for pre-existent and previously unidentified NCDs and risk factors in adolescents and youth enrolled for primary HIV care. Characteristics between sexes and age groups were compared using parametric or non-parametric statistical tests.Results: Three out of four participants were female, and the median age was 20.5 years (IQR 18.9- 22.9). More than a quarter were not in education, employment or training (NEET) and 44% were multidimensionally poor. Our results show an existent burden of hypertension (5%) and central obesity (37%) as well as high levels of depression (43%) and psychological distress symptoms (44%). AYLHIV further self-reported high levels of household food insecurity (70%), low fruit and vegetable consumption, high fast-food and sugar-sweetened beverage intake, low nutritional knowledge and insufficient physical activity. Beyond the NCD risk attributable to HIV and ART, these multiple risk factors further increase NCD risk. Conclusions: Our findings highlight the importance of integrated prevention with NCD risk screening as part of HIV care for AYLHIV and the need for early intervention on social, environmental and economic determinants of NCDs targeting adolescents and youth.