bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustained Delivery of MMP-9 siRNA&nbsp;via Thermosensitive Hydrogel Accelerates Diabetic Wound Healing
Authors: Lan Biyun , Zhang Liming , Yang Liqun , Wu Junfeng , Li Na , Pan Chenglin , Wang Xiaoyi , Zeng Lexiang , Yan Li , Yang Chuan , Ren Meng 
Published: February 2021

Abstract
Excessive expression of matrix metalloproteinase 9 (MMP-9) impedes healing of diabetic chronic wounds, thus wound dressing that could effectively inhibit the expression of MMP-9 offers significant clinical translation for diabetic wound healing. Herein, a hybrid hydrogel dressing was developed for localized and sustained delivery of MMP-9 siRNA (siMMP-9). siMMP-9 was complexed with Gly-TETA (GT), the GT/siMMP9 complex was then loaded into a thermosensitive hydrogel based on Pluronic F-127 (PF) and methylcellulose (MC). In vitro, this hybrid hydrogel dressing exhibited negligible cytotoxicity, prolonged the release of GT/siMMP-9 for up to 7 days, and significantly reduced MMP-9 expression. In vivo assessment in diabetic rats demonstrated that hydrogel provided localized and sustained delivery via the thermosensitive controlled release of entrapped GT/siMMP-9 into wound tissues for 7 days, resulting in dramatic MMP-9 silencing which significantly improved diabetic wound closure. This hybrid hydrogel dressing exhibited excellent biocompatibility, with no observed systemic toxicity in rats. Taken together, the hybrid hydrogel dressing may constitute an effective and biocompatible means of enhancing diabetic wound healing through effective silencing of the MMP-9 gene, and this hydrogel delivery system also offers a platform for in vivo delivery of siRNA for the treatment of other diseases.
Unravelling the Anticancer Potential of a Square Planar Copper Complex: Toward Non-platinum Chemotherapy
Authors: Malik Manzoor Ahmad , Raza Md Kausar , Mohammed Arif , Wani Mohmmad Younus , Hashmi Athar Adil 
Published: February 2021

Abstract
Coordination compounds from simple transition metals are robust substitutes for platinumbased complexes due to their remarkable anticancer properties. In a quest to find new metal complexes that could substitute or augment the platinum based chemotherapy we synthesized three transition metal complexes C1-C3 with Cu(II), Ni(II), and Co(II) as the central metal ions, respectively, and evaluated them for their anticancer activity against human keratinocyte (HaCaT) cell line and human cervical cancer (HeLa) cell lines. These complexes showed different activity profiles with the square planar copper complex C1 being the most active with IC50 values lower than the widely used anticancer drug-cisplatin. Assessment of the morphological changes by DAPI staining and ROS generation by DCFH-DA assay exposed that the cell death occurred by caspase-3 mediated apoptosis. C1 displayed interesting interactions with Ct-DNA, evidenced by absorption spectroscopy and validated by docking studies. Together, our results suggest that binding of the ligand to the DNA-binding domain of p53 tumor suppressor (p53DBD) protein and the induction of apoptotic hallmark protein, caspase-3 upon treatment with the metal complex could be positively attributed to a higherlevel of ROS and the subsequent DNA damage (oxidation), generated by compound C1, that could well explain the interesting anticancer activity observed for this complex.
Relationships between social withdrawal and facial emotion recognition in neuropsychiatric disorders
Authors: Torre-Luque Alejandro , Viera-Campos Alba , Bilderbeck Amy C. , Carreras Maria Teresa , Vivancos Jose , Diaz-Caneja Covadonga M. , Aghajani Moji , Saris Ilja M. J. , Raslescu Andreea , Malik Asad , Clark Jenna , Penninx Brenda W. J. H. , Wee Nic Van der , Rossum Inge Winter-van , Sommer Bernd , Marston Hugh , Dawson Gerard R. , Kas Martien J. , Ayuso-Mateos Jose Luis , Arango Celso 
Published: February 2021

Abstract
This study aimed to investigate the role of social withdrawal in emotion recognition of patients with schizophrenia (SZ) or probable Alzheimer’s disease (AD). A sample of 156 participants was recruited: SZ patients (n = 53), AD patients (n = 46), and two age-matched control groups (SZc, n = 29; ADc, n = 28). All participants provided self-report measures of loneliness and social functioning, and completed a facial emotion detection task. As a result, neuropsychiatric patients (both groups) showed poorer performance in detecting both positive and negative emotions compared with their healthy counterparts (p < .01). Social withdrawal was associated with higher accuracy in negative emotion detection, across all groups. Additionally, neuropsychiatric patients with higher social withdrawal showed lower positive emotion misclassification. Our findings help detail the similarities and differences in social function and facial emotion recognition in two disorders rarely studied in parallel, AD and SZ. Transdiagnostic patterns in these results suggest that social withdrawal is associated with heightened sensitivity to negative emotion expressions, potentially reflecting hypervigilance to social threat. Across the neuropsychiatric groups specifically, this hypervigilance associated with social withdrawal extended to positive emotion expressions, an emotional-cognitive bias that may impact social functioning in people with severe mental illness.
The Systematic Analysis of lncRNA Expression in Ebola-infected Human Macrophages
Authors: Muthaiyan Mathavan , Naorem Leimarembi Devi , Sinnarasan VigneshwarSuriya Prakash , Paul Dahrii , Venkatesan Amouda 
Published: February 2021

Abstract
Ebola is a dangerous virus which causes severe headache, muscle pain, hemorrhagic fever, and multi-organ failure. The underlying mechanism of Ebola-human interaction at the molecular level remains unexplored. The recently reported research revealed that lncRNA are known to be important in the pathogenesis of viruses. The study carried out the comprehensive analysis of lncRNA identification in EBOLA, LPS and RESTV infected MDMs. In EBOLA, the total number of known lncRNA is EBOLA 555 in, in RESTV 476, in LPS 550 and the novel lncRNAs in EBOLA 142, RESTV 127, in LPS 136 identified. Also, differentially expressed lncRNAs and neighbourhood genes of each lncRNA about 100kb identified for analysis.The study reported the known and novel lncRNA in EBOLA, LPS and RESTV infected cells of MDMs. The results of the study may help to understand the immune response further.
New Normal Weather Breaks a Traditional Clothing Retail Calendar
Authors: Oh Jungmi , Ha Kyung-Ja , Jo Young-Heon 
Published: February 2021

Abstract
Background: Clothing businesses have complained of sluggish sales because of new normal weather, an increased variation of temperature and precipitation and the higher occurrence of extreme weather events. Traditionally, the business runs tied to calendar dates or retailing events, and the previous year's sales draw up a sales plan. This study questioned whether the sales planning method of the clothing business is valid and reliable for today. Results: Using weather observation data and Google Trends for the past 11 years, consumers' responses to weather changes were analyzed through the decision tree to learn about consumer insights. The month is the most significant predictor of seasonal clothing demand during a season, and consumers' responses to weather vary from month to month. Minimum temperature and maximum temperature were significant predictors in a particular month. Conclusions: Our results have important managerial implications. Rapid weather changes affect consumers’ demand. Clothing retailers can apply the predictive model to quickly respond to unexpected weather changes, prepare products with rapidly increasing demand not to miss sales opportunities, and adjust quantities and prices for products with sharp declines in demand.
FGF23, A Novel Muscle Biomarker Detected in The Early Stages of ALS
Authors: Si Ying , Kazamel Mohamed , Benatar Michael , Wuu Joanne , Kwon Yuri , Kwan Thaddaeus , Jiang Nan , Kentrup Dominik , Faul Christian , Alesce Lyndsy , King Peter H. 
Published: February 2021

Abstract
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive muscle weakness. Skeletal muscle is a prime source for biomarker discovery since it is one of the earliest sites to manifest disease pathology. From a prior RNA sequencing project, we identified FGF23 as a potential muscle biomarker in ALS. Here, we validate this finding with a large collection of ALS muscle samples and found a 13-fold increase over normal controls. FGF23 was also increased in the SOD1G93A mouse, beginning at a very early stage and well before the onset of clinical symptoms. FGF23 levels progressively increased through end-stage in the mouse. Immunohistochemistry of ALS muscle showed prominent FGF23 immunoreactivity in the endomysial connective tissue and along the muscle membrane. ELISA of plasma samples from the SOD1G93A mouse showed an increase in FGF23 at end-stage whereas no increase was detected in a large cohort of ALS patients. In conclusion, FGF23 is a novel muscle biomarker in ALS and joins a molecular signature that emerges in very early preclinical stages. The early appearance of FGF23 and its progressive increase with disease advancement offers a new direction for exploring the molecular basis or response to the underlying pathology of ALS.
Carboplatin-induced Senescence in Ovarian Cancer Cells Is Reversed Through EGFR&nbsp;and NF-κB&nbsp;Signaling
Authors: Li Yue , Fu Mingxu , Guo Ling , Sun Xiaoxiao , Chen Yuhang , Zhang Tianran , Qin Jinlong , Shao Xiaowen 
Published: February 2021

Abstract
Background: Metastases and recurrence of ovarian cancer after surgery and chemotherapy account for most cancer-related deaths, yet the mechanism underlying metastases and recurrence remains poorly understood. Recent evidence demonstrates that although long-lasting cells were considered tumor suppressors, senescent cancer cells, can induce the metastases and recurrence. In this study, we focused on the fate of ovarian cancer cells treated with carboplatin and explored the mechanism underlying ovarian cancer cell recovery from chemotherapy-induced senescence. Methods: SÁ-β-galactosidase staining was used to detect the impact of carboplatin on senescence of ovarian cancer cells. Cell proliferation was determined using direct cell counting, clone formation assay and 3D tumor spheroid formation assay. Lentivirus-mediated transduction was used to silence or upregulate EGFR expression. Quantitative real-time PCR and western blot analysis validated the efficacy of the knockdown or overexpression effect. Immunofluorescence staining and western blot analysis were used to examined the expression of EGFR and NF-KB. Cell death was determined using trypan blue staining assay. Results: Ovarian cancer cells treated by carboplatin exhibit a senescence-like phenotype indicated by SA-β-galactosidase positive staining. Importantly, carboplatin-induced senescence-like phenotype is reversible. In ovarian cancer cells, EGFR positively regulated cells proliferation, decreased carboplatin-induced senescence and upregulated the NF-κB1 protein level. EGFR/NF-κB1 upregulation promoted the recovery of ovarian cancer cells from senescence and chemoresistance to carboplatin. Conclusions: Ovarian cancer cells treated with carboplatin displayed a reversible senescence-like phenotype that could be combined with EGFR or NF-κB1 inhibitors to improve treatment effects.
High or Low Temperature Extraction, Which Is More Conducive to Triphala Aginst Chronic Pharyngitis?
Authors: Ran fei , Han xue , Deng xuan , Wu Zhenfeng , Huang Hanzhou , Qiu Min , Song Jao , Wei Xichuan , Zhang Dingkun , Wang Yu , Fan Sanhu , Lin Junzhi , Han Li 
Published: February 2021

Abstract
BackgroundTriphala is a world famous herbal formula, its therapeutic effect on chronic pharyngitis has been confirmed in the majority of patients in China. However, the effects of current industrial extraction methods on its anti chronic pharyngitis components and activities are still unclear Methods:The network pharmacology was used to analyze the material basis, targets and pathways of Triphala for chronic pharyngitis. HPLC were used to compare the fingerprint profile and content of components between the two extracts. The antioxidant and anti chronic pharyngitis activities of the two extracts were compared by DPPH assay and ammonia induced chronic pharyngitis model in rats. Results:The network pharmacology results showed that the active ingredients of Triphala for chronic pharyngitis are epigallocat echin 3 gallate catechin , epicatechin , epicatechin gallate , g allocatechin , quercetin luteolin leucodelphinidin and other flavonoids, phenolic acids such as gallic acid and ellagic acid, alkaloids such as ellipticine , cheilanthifoline, and hydrolyzed tannins such as corilagin and chebulic acid . The high temperature reflux extract and the low temperature decompressing inner ebullition extract have extremely significant differences in the fingerprint profile. Among them, the content of 8 active ingredients of gallic acid, ellagic acid, chebulic acid , catechin, epicatechin, corilagin, quercetin, and epicatechin gallate in the reflux extract is 1.1 to 5.3 times as much as decompressing inner ebullition extract. The free radical scavenging ability of reflux extract is significantly stronger than that of decompression extract (p<0.01), and it has a repairing effect on pharyngeal mucosal damage (reducing keratinization or hyperplasia of mucosal epithelium, reducing inflammatory cell infiltration and bleeding), and reducing IL 1β (P 0.05), IL 6 (p 0.05), TNF α overexpression ability is stronger than the decompressing inner ebullition extract. Conclusions:gallic acid, ellagic acid, chebulic acid , catechin, epicatechin, corilagin and epicatechin gallate are the basic aglycones or oligomers of tannin. and high temperature reflux extraction can significantly promote temperature reflux extraction can significantly promote tthe occurrence of the he occurrence of the hydrolysis of tannins significantly increases the content of hydrolysis of tannins significantly increases the content of these components, and the these components, and the antianti--chronic pharyngitis activity is enhanced. chronic pharyngitis activity is enhanced. It is suggested that high temperature It is suggested that high temperature reflux extraction should be used in the treatment of chronic phreflux extraction should be used in the treatment of chronic pharyngitis.aryngitis.
Ropivacaine Induce G0 / G1 Phase Cell Cycle Arrest and Apoptosis of PC12 Cells Via Inhibiting Mitochondrial STAT3 Translocation
Authors: Zeng Lian , Zhang Zhen , Zhang Fuyu , Liu Chenguang , He Jiafeng , Chen Huaxian , Wang Qingsong , Ding Xudong , Luo Huiyu 
Published: February 2021

Abstract
Signal transducer and activator of transcription 3 (STAT3) has been shown to have neuroprotective effects via the non-canonical activation and mitochondrial translocation, but its effect on ropivacaine induced neurotoxicity is unclear. Our previous studies have revealed that apoptosis was an important mechanism of ropivacaine induced neurotoxicity, so this study is to explore the relationship between STAT3 with ropivacaine induced apoptosis. Our results showed that ropivacaine decreased PC12 cell viability, arrested the cells in the G0 / G1 phase, induced cell apoptosis and oxidative stress, and damaged mitochondrial functions. In addition, the serine727 (Ser727) phosphate activation of STAT3 was inhibited by ropivacaine. Ropivacaine decreased the p-STAT3 (Ser727) expression in the mitochondria, and increased p-STAT3 (Ser727) expression in the cytoplasm, so that it inhibited mitochondrial STAT3 translocation. To explore the potential connection between STAT3 with ropivacaine, the autodock-vina was used to examine the interaction between STAT3 and ropivacaine, and results showed that ropivacaine could bind to STAT3. Furthermore, the apoptosis of the spinal cord was increased and the p-STAT3 (Ser727) expression in the spinal cord was reduced after ropivacaine treatment. our findings illustrated that ropivacaine induced neurotoxicity by increasing the apoptotic levels, the activation and mitochondrial translocation of STAT3 were inhibited by ropivacaine. There may be an internal connection between these phenomena.
Significant Benefit of Everolimus In a Patient With Urothelial Bladder Cancer Harboring A Rare M1043I Mutation of PIK3CA
Authors: Pan Shouhua , Li Si , Xiao Mingzhe , Chen Dongsheng , Li Junlong 
Published: February 2021

Abstract
Urothelial bladder cancer (UBC) is a common malignancy with significant mortality worldwide. However, treatment options of UBC were mainly chemotherapy and immunotherapy since few targeted agents had shown efficacy in UBC. In recent studies, everolimus has showed antitumor activity in patients harboring aberrations in PI3K/Akt/mTOR pathway in multiple tumor types. Here we report a patient with metastatic UBC harboring a rare M1043I mutation of PIK3CA detected by DNA based next-generation sequencing. The patient received everolimus as first-line therapy after palliative transurethral resection. Within one months, the residual lymph node metastases achieved complete response and no more ostealgia was reported. To our knowledge, this is the first case reporting a significant benefit from everolimus in PIK3CA mutant UBC, suggesting the rare M1043I mutation variant may be a potential biomarker of sensitivity to everolimus. Further mechanism insights and clinical studies are needed to clarify the effectiveness of everolimus in patients with PIK3CA M1043I mutation.
An Original Aspirin-Containing Carbonic Anhydrase 9 Inhibitor Overcomes Hypoxia-induced Drug Resistance to Enhance The Efficacy of Myocardial Protection
Authors: Zhou Wen , Zhang Bin , Fan Keyu , Yin Xiaojian , Liu Jinfeng , Gou Shaohua 
Published: February 2021

Abstract
Purpose Hypoxic microenvironment plays a vital role in myocardial ischemia injury, generally leading to the resistance of chemotherapeutic drugs. This induces an intriguing study on mechanism exploration and prodrug design to overcome the hypoxia induced drug resistance.Methods In this study, we hypothesized that the overexpression of carbonic anhydrase 9 (CAIX) in myocardial cells is closely related to the drug resistance. Herein, bioinformatics analysis, gene knockdown and overexpression assay certificated the correlation between CAIX overexpression and hypoxia. An original aspirin-containing CAIX inhibitor AcAs has been developed.Results Based on the downregulation of CAIX level, both in vitro and in vivo, AcAs can overcome the acquired resistance, and more effectively attenuate myocardial ischemia and hypoxia injury than that of aspirin. CAIX inhibitor is believed to recover the extracellular pH value so as to ensure the stable effect of aspirin.Conclusion Results indicate great potential of CAIX inhibitor for further application in myocardial hypoxia injury therapy.
The Impact of Ossification Spread on Cervical Spine Function in Patients With Ossification of The Posterior Longitudinal Ligament
Authors: Katsumi Keiichi , Hirai Takashi , Yoshii Toshitaka , Maki Satoshi , Mori Kanji , Nagoshi Narihito , Nishimura Soraya , Takeuchi Kazuhiro , Ushio Shuta , Furuya Takeo , Watanabe Kei , Nishida Norihiro , Watanabe Kota , Kaito Takashi , Kato Satoshi , Nagashima Katsuya , Koda Masao , Ito Kenyu , Imagama Shiro , Matsuoka Yuji , Wada Kanichiro , Kimura Atsushi , Ohba Tetsuro , Katoh Hiroyuki , Matsuyama Yukihiro , Ozawa Hiroshi , Haro Hirotaka , Takeshita Katsushi , Watanabe Masahiko , Matsumoto Morio , Nakamura Masaya , Yamazaki Masashi , Okawa Atsushi , Kawaguchi Yoshiharu 
Published: February 2021

Abstract
Ossification of the posterior longitudinal ligament (OPLL) is a progressive disease. The bridging of ossified lesions to the vertebral body gradually increases, thereby decreasing the mobility of the cervical spine; thus, cervical spine function may decrease over time. However, cervical spine function in patients with cervical OPLL has not been evaluated in large prospective studies. Therefore, we conducted a prospective multicenter study to clarify whether ossification spread can influence cervical spine function and quality of life (QOL) in patients with cervical OPLL. In total, 238 patients (162 men, 76 women; mean age, 63.9 years) were enrolled from 16 institutions. Each patient underwent whole spine computed tomography and was evaluated cervical spine function and QOL using the Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire (JOACMEQ). In the multivariate regression analysis, a higher neck VAS score and a larger number of bridge formations of OPLL in the whole spine were significant predictors of adverse outcomes related to cervical spine function. This is the first prospective multicenter study to reveal the impact of ossification spread on cervical spine function. These findings are important to understand the natural course of OPLL and can serve as controls when evaluating postoperative cervical spine function.
Faradarmani Consciousness Field Suppresses Alzheimer’s Disease Development in Both in Vitro and in Vivo Models of The Disease
Authors: Taheri Mohammad Ali , Torabi Sara , Nabavi Noushin , Semsarha Farid 
Published: February 2021

Abstract
Background: Alzheimer’s Disease (AD) is one of the most common causes of dementia, imposing large financial and psychological burdens on nations worldwide. Thus, we are in dire need for new treatment strategies or drugs for this disease. The aim of this study is to investigate the effects of a novel non-pharmacological method in the treatment of Alzheimer’s disease, based on Taheri's Consciousness Field approach. This approach works at the level of cellular and molecular processes and is tested in neuronal cells of AD mouse models. Methods: In this study, we established a neuron cell culture from an AD mouse model as well as a traumatic brain injury mouse model. We then measured changes in amyloidopathy, tau protein content, microtubule assembly, neuronal cell survival, and finally behavior of TBI mice in elevated Plus Maze under treatment of the Faradarmani Consciousness Field (FCF). Results: According to the results of this study, treatment of neural cell and mouse model of Alzheimer's disease by the FCF, leads to about complete survival of neural cell models and elimination of amyloidopathy and tau protein and remarkable behavioral improvement of the treated TBI mice model in the elevated plus maze. Conclusions: Based on the results, the FCF treatment suppresses AD development in the laboratory models. In this regard, conducting a human clinical study with the aim of introducing a new global complementary and alternative medicine in AD treatment is highly recommended.