bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of 1-naphthylacetic and indol-3-acetics acid on the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro
Authors: Mishchenko S. V. 
Published: October 2021

Aim. Investigate the effect of auxins of exogenous origin in nutrient medium in vitro on the germination and organogenesis intensity in Linum usitatissimum L. convar. elongatum (‘Hlinum’ variety) at the constant concentration of 6-benzylaminopurine (BAP). Methods. Hypocotyl segments were cultured on Murashige and Skoog nutrient medium supplemented with sucrose (30 g/l) and phytohormones at various concentrations. Other conditions: photoperiod 16 hours, relative humidity 60–80%, air temperature 22–24°C. Results. Common flax has a great capacity to form callus and shoots under the effect of the following factors: 1) only auxins, 2) only cytokines, 3) combinations of auxins and cytokines. Somatic embryogenesis is also possible on a nonhormonal nutrient medium. Conclusions. For somatic embryogenesis in vitro, the optimal concentrations of BAP can be expressed as 1.0 ≤ BAP ≤ 1.75, the optimal concentrations of BAP for the medium supplemented with 1-naphthylacetic (NAA, 0.05 mg/l) 0.5 ≤ BAP ≤ 2.0, the optimal concentration of NAA for the medium supplemented with BAP (1.0 mg/l) 0.025 ≤ NAA ≤ 0.150, and the optimal concentrations of indol-3-acetics acid (IAA) for the medium supplemented with BAP (1.0 mg/l) 0.05 ≤ IAA ≤ 0.50. Keywords: Linum usitatissimum L., in vitro, phytohormones, callus, organogenesis.
Application of nanomaterial for maturation in vitro of cattle embryos
Authors: Trotskyi P. A. , Shcherbak O. V. , Kovtun S. I. 
Published: October 2021

Aim. To evaluate the effectiveness of the use of nanomaterial in the environment for the further development of in vitro embryos derived from frozen-thawed oocytes in the system of conservation of genetic resources of animals at the cellular level. Methods. Biotechnological, cryobiological, morphological, cytogenetic, and statistical methods, as well as methods of statistical data processing were used in the research. Results. Oocyte-cumulus complexes (OCC) of cows were divided into four groups: three experimental, in which the maturation was performed in a medium containing 0.1, 0.01 and 0.001% UFS/sucrose and control - without the addition of nanobiomaterial. In vitro fertilization of pre-mature frozen-thawed ova of cows and subsequent maturation of embryos in the medium with the addition of UFS/sucrose (0.001%) showed an increase in the number of embryos by 16.7-22.1% compared with the addition of 0.1; 0.01% and 13.1% compared to the control group. It was found that the fragmentation rate of 2-cell cattle embryos decreased from 65.0 to 39.8% with a decrease in the concentration of UFS/sucrose from 0.1 to 0.001%. The most stable indicators of the fragmentation index from 78.4 to 50.0% were observed on the fourth day of embryo cultivation in experimental group B. Conclusions. Reducing the concentration of UFS/sucrose from 0.1 to 0.001% in the composition of the medium for in vitro maturation of cattle embryos leads to an increase of 16.7-22.1% in the number of embryos obtained.
Keywords: oocyte-cumulus complex, cryopreservation, nanomaterial, in vitro maturation, embryo.
The application of biostimulants Regoplant and Stimpo to increase wheat resistance to salinity conditions
Authors: Tsygankova V. A. , Spivak S. I. , Shysha O. M. , Pastukhova N. L. , Yemets A. I. , Blume Ya. B. 
Published: October 2021

aim. Investigation of bioprotective action of polycomponent biostimulants of natural origin Regoplant and Stimpo according to physiological and molecular genetic parameters of resistance of winter wheat plants (Triticum aestivum L.) varieties Antonivka ta Hoduval'nytsya odes'ka to the salinity conditions. Methods. The physiological parameters of resistance of experimental wheat plants obtained from seeds treated and untreated with solutions of Regoplant and Stimpo biostimulants used at concentrations of 50 μl/L and 75 μl/L grown under salt stress on 0,1 M sodium chloride solution were studied. Using Dot blotting method the degree of hybridization between cytoplasmic si/miRNA isolated from cells of experimental wheat plants grown under salinity conditions and mRNA of control plants grown in the absence of salinity conditions was determined. In the wheat embryo cell-free system of protein synthesis in vitro the silencing activity of si/miRNA on the template of mRNA isolated from cells of experimental wheat plants grown under salt stress conditions and control plants was studied. Results. It was found that the use of biostimulants Regoplant and Stimpo by soaking the seeds of wheat plants for 24 hours at concentrations of 50 μl/L and 75 μl/L improved the physiological parameters of wheat plants grown under salt stress conditions, which increased on average: seed germination – up to 69-75%, the length of seedlings – up to 28-37%, the length of roots – up to 41-42%, leaf area – up to 25-38%, the raw weight of seedlings – up to 48-53%, the dry weight of seedlings – up to 37-46 %, respectively, compared to control. The Dot-blot hybridization method showed that the largest decrease in the percentage of hybridized mRNA and si/miRNA molecules was observed in experimental wheat plants grown from seeds treated with Regoplant and Stimpo biostimulants under salt stress conditions - up to 66-75%, respectively, compared to control. The increase of silencing activity of si/miRNA on the template of mRNA isolated from experimental wheat plants obtained from seeds treated with Regoplant and Stimpo biostimulants and grown under salt stress conditions was shown to be 39-42%, respectively, compared to control. Conclusions. The use of biostimulants Regoplant and Stimpo in concentrations of 50 μl/L and 75 μl/L for presowing seed treatment contributes to the increase of the resistance of winter wheat plants to salinity conditions.
Keywords: Triticum aestivum L., polycomponent biostimulants, resistance of wheat to salinity conditions, RNA interference.
Structure-based virtual screening for new lead compounds targeted Plasmodium α-tubulin
Authors: Rayevsky O. V. , Demchyk O. M. , Karpov P. A. , Ozheredov S. P. , Spivak S. I. , Yemets A. I. , Blume Ya. B. 
Published: October 2021

Aim. Search for new dinitroaniline and phosphorothioamide compounds, capable of selective binding with Plasmodium α-tubulin, affecting its mitotic apparatus. Methods. Structural biology methods of computational prediction of protein-ligand interaction: molecular docking, molecular dynamics and pharmacophore analysis. Selection of compounds based on pharmacophore characteristics and virtual screening results. Results. The protocol and required structural conditions for target (α-tubulin of P. falciparum) preparation and correct modeling of the ligand-protein interaction (docking and virtual screening) were developed. The generalized pharmacophore model of ligand-protein interaction and key functional groups of ligands responsible for specific binding were identified. Conclusions. Based on results of virtual screening, 22 commercial compounds were selected. Identified compounds proposed as potential inhibitors of Plasmodium mitotic machinery and the base of new antimalarial drugs.
Keywords: malaria, Plasmodium, intermolecular interaction, dinitroaniline derived, phosphorothioamidate derived.
Analysis of interaction mechanisms between main objects of tuberculosis-target therapy and corresponding selective inhibitors
Authors: Samofalova D. O. , Rayevsky O. V. , Ozheredov S. P. , Spivak S. I. , Stykhylias m. m. , Ozheredov D. S. , Karpov P. A. 
Published: October 2021

Aim. Search for new inhibitors of the mitotic apparatus of mycobacterium and a number of enzymatic targets. Methods. 3D models of key targets reconstruction and geometry optimization and analysis of biologically active conformations of inhibitors were performed according to a previously developed technique. Results. A revision of mycobacterial inhibitors, which exhibit antimicrobial action against representatives of the genus Mycobacterium, was carried out, which made it possible to create an appropriate reference library of compounds. The complete spatial structure of a number of the main targets of targeted therapy for tuberculosis was reconstructed and verified, and the features of their interaction with selective inhibitors were established. Chemogenomic profiling was performed, which made it possible to draw conclusions regarding the uniqueness of the studied sites and the potential toxicity of compounds related to these sites for humans. Conclusions. A well-developed search algorithm for known inhibitors of proteins with M. tuberculosis allows further study of the features of their interaction with the corresponding homologues of M. bovis and the development of new, more selective compounds using molecular dynamics and docking methods.
Keywords: tuberculosis, in silico, anti-tuberculosis drugs.
Alexander Fleming (to the 140th anniversary of his birth)
Authors: Voloshyn O. S. , Humeniuk H. B. , Chen I. B. 
Published: October 2021

Alexander Fleming is a prominent Scottish scientist, Nobel laureate, and bacteriologist who discovered penicillin. He was born near the small town Darwell in Scotland. He studied at St. Mary’s Hospital Medical School and received his master’s and bachelor’s degrees from London University. The research of the young scientist began with the study of the etiology, diagnosis and vaccine therapy of infectious diseases, he soon began working on finding treatment options for patients with bacterial infections with a talented military doctor Almroth Wright. Working in the Royal Military Medical Service during the First World War, A. Wright and O. Fleming showed that the use of antiseptics after injury does not destroy bacterial pathogens effectively. After the war, O. Fleming made an extremely important discovery of a lytic enzyme with a pronounced antibacterial effect - lysozyme. This discovery prompted him to search for antibacterial drugs actively. In 1928, Alexander Fleming discovered the first antibiotic – penicillin. He found that penicillin is an effective antibacterial substance that can affect pyogenic cocci and diphtheria bacilli. The widespread introduction of penicillin into clinical practice has saved hundreds millions of lives.
Keywords: penicillin, lysozyme, antibiotics, bacterial infections.
Educational and scientific aspects of history of genetics and selection at The Sumy State Pedagogical University named after A.S. Makarenko (1960–1990)
Authors: Torianyk V. M. , Mironets L. P. 
Published: October 2021

The article is dedicated to the 90th anniversary of the foundation of the Faculty of Natural Geography at the Sumy State Pedagogical University named after A.S. Makarenko. The article focuses on the primary aspects of educational and scientific work on genetics and selection performed by the lecturers of the Botany Department – Professor Nina Ivanivna Dehtiarova and Professor Mykhailo Ivanovych Steblianko, docent Klavdia Demianivna Honcharova and docent Maiia Havrylivna Shulha during the 1960s–1990s. Their contribution to the establishment and development of genetics and selection at higher pedagogical and secondary education institutions in Ukraine was analysed. The importance of Ukrainian genetics and selection for historiography as well as popularisation of scientific and pedagogical accomplishments of lecturers in higher education institutions, who are engaged in basic training of future genetics and selection specialists, was denoted.
Keywords: genetics, selection, history of the Faculty of Natural Geography, Sumy State Pedagogical University named after A.S. Makarenko.
Manifestation and variability of biometric characteristics in line-parental components and hybrids of maize using different genetic peninsular
Authors: Vozhegova R. A. , Lavrynenko Yu.O. , Marchenko T.Y. , Bazalii V.V. 
Published: October 2021

Aim. To establish the manifestation and variability of the trait "plant height" in the lines-parent components and hybrids of corn using different genetic plasmas, to determine the level of heterosis in newly created test crosses and to determine the influence of morphometric parameters on grain yield under irrigation. Methods. General scientific, special, comparative-analytical, regression, information-logical analysis and mathematical modeling. Results. The manifestation and variability of traits "plant height" and "height of cob attachment" in the lines - parent components and hybrids of corn with the use of different genetic plasmas were established. The level of heterosis in newly created test crosses under irrigation conditions was determined. The dependences of biometric parameters and their correlation with grain yield in the test of hybrids obtained with the participation of newly created initial forms of different embryonic plasmas were established. Conclusions. The ratio of plant height of hybrids by maturity groups and yield level showed that for the middle-early group, in the phase of cessation of linear growth, the optimal plant height is 235–265 cm, grain yield is 12.98–13.81 t/ha; for the middle-ripe group - 255–257 cm with grain yield at the level of 15.17–15.82 t/ha. For medium-late hybrids, the optimal plant height to ensure the highest grain yield (over 15 t/ha) is in the range from 270 to 280 cm.
Keywords: parental lines, genetic plasmas, hybrids, maize.
Combining ability of corn inbreds – carriers of mutations Su1 and Su2 on the content of oleic acid glycerides
Authors: Tymchuk D. S. , Potapenko G.S. , Tymchuk N.F. , Kuznetsova I.K. 
Published: October 2021

Aim. Evaluation of donors’ properties of corn inbreds - carriers of endospermic mutations su1 and su2 on the oleates content. Methods. A series of hybrids obtained in top- crosses of 10 inbreds of the common type with four testers – low-oleic and high-oleic inbreds of the common type and inbreds – carriers of the su1 and su2 mutations were analyzed. Oleates’s content was determined by the gas chromatographic method. Results. The highest effects of the general combining ability were shown by the inbreds obtained from high-oil synthetics and the inbreds - carriers the su1 and su2 mutations. Hybrids of low-oleic inbreds with the sources of su1 and su2 mutations had an increased content of oleates in comparison with maternal forms, and hybrids of high-oleic inbreds with the sources of these mutations inherited the oleates content in an intermediate type. In F2 hybrids from crosses of inbreds of the common type with the inbreds - carriers of the su1 and su2 mutations, transgressions were observed. Conclusions. The increased content of oleates in corn inbreds inbreds and hybrids is most likely controlled by oleate - coding loci of chromosome 4 and 6 linked to mutant genes su1 and su2. The carriers of these genes can be used as sources of increased oleate content in the corn breeding for oil quality.
Key words: Zea mays L., endospermic mutants, oleates content, top-crosses
Impact of NaCl on physiological and biochemical characteristics of maize (Zea mays L.) plants
Authors: Tagieva K. R. , Azizov I. V. 
Published: October 2021

Aim. The effect of 50 and 100 mmol NaCl concentrations on seed germination, the content of green pigments in seedlings, and on the activity of photosystem II in seedlings of maize varieties Zagatala 420, Zagatala 514, Zagatala 68 and Gurur and hybrid Gurur x Zagatala 68 was studied. Methods. Plant seeds were germinated under laboratory conditions in Petri dishes and pots with soil using 50 and 100 mmol of sodium chloride solutions. Germination, seed germination energy had been determined. In two-week old seedlings determined the number of photosynthetic pigments and the activity of photosystem II. Results. High concentrations (100 mmol) of NaCl reduced seed germination, pigments number, and photosystem II activity. At a salt concentration of 100 mmol, an increase in the carbohydrate content was noted. Conclusions. Varieties Gurur and Zagatala 68 and hybrid Gurur x Zagatala 68 were more tolerant compared to varieties Zagatala 420, Zagatala 514.
Keywords: maize, NaCl, photosynthetic pigments, photosystem II.
Estimation of genotypic features of effects of plant treatment with growth regulators on canopy parameters and yield of winter wheat
Authors: Priadkina G. A. , Stasik O.O. , Makharinska N.M. , Kondratskaya I.P. 
Published: October 2021

Aim. A study of the effects of growth regulators derived from natural raw materials on the photosynthetic apparatus surface and the yield of winter wheat. Methods. Morphometric, spectrophotometric, statistical. Results. An increase in the value of canopy chlorophyll index (ChlI) of Smuhlianka and Astarta varieties under the treatment of plants with Stimpo growth stimulator was established at anthesis, a positive effect of the regulator Regoplant – only for Smuhlianka variety at milk-wax ripeness. Chlorophyll photosynthetic potential (ChlP) during the period anthesis – milk-wax ripeness increased significantly under the use of growth regulators in both varieties, while the efficiency of treatments had varietal differences: growth was greater (11–18% compared to control) in the variety Smuhlianka than in the variety Astarta (6–7%). A positive correlation was established between the yield and ChlP during the period anthesis – milk-wax ripeness. Under the action of treatment of plants with growth regulators, the increase in grain yield was significant (0.29–0.45 t/ha) only in the variety Smuhlianka. Conclusions. The positive effect of treatments of plants with growth regulators, obtained from natural raw materials, on the value of canopy ChlI at anthesis and ChlP during period anthesis – milk-wax ripeness was established. A positive correlation between the winter wheat yield and ChlP during the period anthesis – milk-wax ripeness was found. Varietal differences in the effect of growth regulators treatment of plants on ChlP and yield were found.
Keywords: Triticum aestivum L., grain productivity, chlorophyll index, chlorophyll photosynthetic potential, growth regulator.
Initial developmental rate delay in modern highly productive cultivars of winter bread wheat from West Europe
Authors: Stelmakh A. F. , Litvinenko N.A. , Fait V.I. 
Published: October 2021

Aim. To evaluate the vernalization requirement duration and sensitivity to photoperiod in the set of modern high yielding winter bread wheat cultivars from West Europe which are proposed for production in Ukraine. Methods. Planting in natural and shortened photoperiods followed by artificial vernalizing variants of 5-days green seedlings have been used for heading dates registration. Results. Many foreign cultivars revealed the durable vernalization requirement and strong photosensitivity. Few stocks were characterized by the week analogous physiological reactions, which are typical in most modern Ukrainian cultivars and breeding lines. Conclusions. Reveled data confirmed the adaptive value of discussed properties for specific environment which are similar to Ukrainian one in many zones of winter wheat cultivation. And they refute the assertion that strong photosensitivity and durable vernalization requirement limit the improvement of yield potential by selection.
Keywords: winter bread wheat, vernalization requirement, photosensitivity, modern cultivars from West Europe.
Differentiation of eiincorns by water retention capacity
Authors: Fu Hao , Lyutenko V.S. , Zhmurko V.V. , Bohuslavskyi R. L. 
Published: October 2021

Aim. On the basis of multivariate analysis, to differentiate einkorn wheat accessions by the water-holding capacity of leaves and ears and the parameters of these organs at the level of species and genotypes. Methods. The water-holding capacity was assessed by the moisture-yielding coefficient which was determined by the method of N.N. Kozhushko (Kozhushko, 1988). Differentiation of einkorn samples was carried out by the method of principal components in the interpretation of A.V. Korosov (Korosov, 1996). Results. Triticum monococcum UA0300113, Syria, and UA0300282, Hungary were characterized by the lowest specific moisture-yielding coefficients: for the second and flag leaves, respectively, (mg / cm2) 10.9, 7.0 and 11.4, 10.7; ears – the same samples as well as Triticum boeoticum UA0300401, Ukraine-Crimea: (mg / cm) 29.6, 28.2, 25.4. With an increase in the size of the lamina, there is a tendency to a decrease in its specific moisture yield. The samples of eincorn are differentiated into 4 clusters corresponding to the species. Conclusions. T. monococcum UA0300113, Syria, and UA0300282, Hungary are relatively drought tolerant. The complex of features characterizing leaf and spike size in combination with the moisture-yielding coefficients processed by the method of principal components makes it possible to differentiate einkorn oaccessions by species and genotype.
Keywords: einkorns, moisture yield, drought resistance, leaf, ear.
Evaluation of population frequency for age-dependent multifactorial disorders
Authors: Atramentova L. O. , Utevska O.M. 
Published: October 2021

Aim. Description of the method to calculate the population incidence of age- and sex-dependent multifactorial diseases. Methods. For the analysis, we used statistical material of psychiatric hospitals in the Kharkiv region for 2016. Calculation of the population frequency was carrying out according to the methodology used in demographic studies. Results. In medical genetics, population frequency is mainly used for prognostic purposes to assess the genetic load of a population or to calculate the probability to inherit a disease. Evaluation of the population frequency of multifactorial disease is complicated by varying age of onset, differential survival, different thresholds of hereditary predisposition for men and women. Prevalence, which is often used instead population frequency, is not a gene pool characteristic and is not useful for genetic analysis and risk assessment. The population frequency, calculated for affective disorders by the proposed method (0.184%), is 1.33 times higher than the prevalence rate (0.138%), that is, a third of cases when using the prevalence turns out to be lost that distorts the derived genetic indicators. Conclusions. For the correct evaluation of the population frequency, the age-specific incidence for two sexes separately must be estimated, followed by the calculation of the cumulative frequencies.
Keywords: multifactorial diseases, prevalence, morbidity, population frequency.