bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of Diabetes Self-Management Behaviors in Type 2 Diabetes Patients
Authors: Azami Golnaz , Kim Lam Soh , Shariff-Ghazali Sazlina , Mohd Said Salmiah , Aazami Sanaz , Mozafari Mosayeb 
Published: January 2021

Objective: Type 2 diabetes mellitus (T2DM) is a chronic condition that requires consistent medical care to help control glycemic indices. Diabetes self-management is found to be essential for optimal glycemic control. This study aimed to investigate the predictors of diabetes self-management in adult with T2DM.
Materials and Methods: A cross-sectional study was conducted. A purposive sample of 142 adults with T2DM attended an outpatient endocrine clinic in an academic hospital in Ilam, Iran was invited to participate in this study from September to October 2016. The data were collected using a combination of validated questionnaires and the blood sample. IBM SPSS software version 22 used to conduct the analysis. Hierarchical linear regression analysis with the stepwise method was used to explore the predictors of diabetes self-management.
Results: The mean age of participants was 54.2 ± (11.8) years. The mean duration of diabetes was 8.9 ± (7.4). Hierarchical linear regression analysis determined that self-management behaviors had positive relationship with efficacy expectation (B= 0.445, P-value< 0.01), quality of life (B= 0.222, P-value= 0.02), and has a negative relationship with HbA1c (B= -0.194, P-value= 0.01).
Conclusion: The result of our study indicate that better diabetes self-management behaviors can be predicted by higher efficacy expectation, the better quality of life and lower HbA1c levels. Future interventions should focus on enhancing efficacy expectation, quality of life and optimizing glycemic control to improve self-management of diabetes.
The Effect of High Intensity Intermittent Training (HIIT) on GLP-1R Expression in Pancreas Tissue and Serum Insulin of Male Wistar Rats with Type 2 Diabetes Mellitus
Authors: Karimi Mohammad , Eizadi Mojtaba 
Published: January 2021

Objective: Insulin secretion and insulin resistance are associated with incidence and severity of type 2 diabetes mellitus (T2DM). This study aimed to assess the effect of high intensity intermittent training (HIIT) on GLP-1R expression in pancreas tissue and serum insulin of T2DM rats.
Materials and Methods: In this quasi-experimental study, T2DM induced by intraperitoneal injection of streptozotocin-Nicotinamide in 16 male wistar rats aged 10 weeks (220 – 20 g). The rats were divided into control (no-training, n= 8) and HIIT (5 times/ week/12-week, n= 8) groups. GLP-1R expression in pancreas tissue, fasting glucose and serum insulin were measured 48 h after lasted exercise of exercise and control groups. Independent and paired T-test was used to compare variables between 2 groups.
Results: HIIT resulted in significant increase in GLP-1R expression compared to control subjects (P-value: 0.023). Fasting glucose decreased (P-value< 0.0001) and serum insulin increased (P-value< 0.0001) in response to HIIT when compared to control subjects.
Conclusion: HIIT can improve insulin secretion in male rats with T2DM and this improvement can be attributed to increased GLP-1R expression in pancreas tissue in response to training protocol.
Genetic Diversity Analysis among 27 Indian Goat Populations using Microsatellite Markers
Authors: Sah Rajkumar , Dixit Santpal 
Published: January 2021

Background: Livestock genetic diversity studies focus on their within and diversity, breed history, adaptive variations, ancestral information, site of domestication and parentage testing and assess the genetic uniformity, admixture or subdivision, inbreeding, or introgression in the population which is helpful in breed formation and their sustainable utilization. Methods: The present research work was conducted during the year 2016-17 at National Bureau of Animal Genetics Resources, Karnal-132001. STR data of 25 markers on 1237 random samples of 27 goat populations was used for analysis. The genetic diversity analysis of new population viz: Narayanpatna, Raighar, Kalahandi, Malkangiri of Odisha state and Rohilkhandi (UK) and their association studies with other Indian goat breeds was performed. Result: It was found that used markers are highly polymorphic- and the studied breeds/population showed great diversity and distributed mostly on the basis of physio-geographical condition and type of production but among new populations diversity was least which might be due to exchange of animal for breeding purposes. The studied new goat populations were well differentiated from other goat breeds which might be due to physio-geographical condition and breeding practices, so these may be considered as separate breeds/populations. In conclusion, the results showed high level of conserved genetic diversity in the Indian goat breeds. The smaller and isolated new population showed less diversity and a higher inbreeding level as compared to registered breeds.
Genetic Enhancement and Genetic Analysis of Resistance to Asian Soybean Rust in India
Authors: Immadi Shobha , Basavaraja G.T. , Suresh P.G. 
Published: January 2021

Background: Asian soybean rust caused by Phakopsorapachyrhizi Syd. is a major threat for production and expansion of soybean in India. Understanding the nature of inheritance of rust resistance in DSb 21 will help in estimating the number of genes involved in governing the resistance mechanism and thus will be helpful in designing a suitable breeding method. Methods: Utilizing JS 335 and EC 241778, in a long term breeding programme, a highly rust resistant variety, DSb 21 has been released from University of Agricultural Sciences Dharwad, Karnataka. To study the genetics of rust resistance in DSb 21, F2 and F3 generation of two crosses involving susceptible x resistant (JS 335 x EC 241778) and resistant x susceptible (DSb 21 x JS 335) were used. Result: The F2 segregation analysis in both the crosses revealed the same segregation ratio of three resistant to one susceptible phenotype based on chi square test indicating rust resistance is controlled by a single dominant gene. In both the crosses the F2 results were confirmed by studying the F3 progenies for reaction to rust.
Pengaruh ukuran perusahaan dan leverage terhadap nilai perusahaan dengan profitabilitas sebagai variabel moderasi
Authors: Khotimah Siwi Nur , Mustikowati Rita Indah , Sari Ati Retna 
Published: January 2021

This study aims to examine and explain the effect of company size and leverage on firm value with profitability as a moderating variable in Real Estate and Property companies listed on the Indonesia Stock Exchange in the period 2016-2018. This type of research is explanatory research, testing classical assumptions, and analyzed using a moderated regression analysis, and using the t test. The number of samples is 32 companies, and the sampling method used is purposive sampling. This research variable consists of company size and leverage as an independent variable, company value as the dependent variable, and profitability as a moderating variable. The analysis showed that partially company size and leverage had no significant effect on firm value, profitability had a negative effect on firm value and profitability weakened the effect of company size on firm value and profitability strengthened the effect of leverage on firm value. In this study, it can provide implications for a company to consider factors of company size, leverage, and profitability, and can also be used as a reference by other companies in business strategy, understand aspects of the industry they are involved in, and pay more attention to the development of the environment that can affect the company's business so that it can increase the value of the company.
Development of a Gene Signature Associated with Iron Metabolism in Lung Adenocarcinoma
Authors: Qin Junqi , Xu Zhanyu , Qin Fanglu , Wei Jiangbo , Yuan Liqiang , Sun Yu , Deng Kun , Zheng Tiaozhan , Li Shikang 
Published: January 2021

Abstract
Background: There are few studies on the role of iron metabolism genes in predicting the prognosis of lung adenocarcinoma (LUAD). Our research aims to screen key genes and to establish a prognostic signature that can predict the overall survival rate of lung adenocarcinoma patients. Methods: Genes related to iron metabolism were downloaded from the GeneCards database; in addition, RNA-Seq data and corresponding clinical materials of 594 adenocarcinoma patients from The Cancer Genome Atlas(TCGA) were downloaded. GSE42127 of Gene Expression Omnibus (GEO) database was also further verified. The multi-gene prognostic signature was constructed by the Cox regression model of the Least Absolute Shrinkage and Selection Operator (LASSO). The clinical applicability of the model and its connection with immune cell infiltration was then analyzed. Results: We constructed a prediction signature with 12 genes (HAVCR1, SPN, GAPDH, ANGPTL4, PRSS3, KRT8, LDHA, HMMR, SLC2A1, CYP24A1, LOXL2, TIMP1) in the TCGA test set, and counted the patient's risk value based on this 12-gene signature; patients were split into high and low-risk groups. The survival graph results revealed that the survival prognosis between the high and low-risk groups was significantly different (TCGA: P <0.001, GEO: P = 0.001). Univariate and multivariate Cox regression analysis confirmed that the risk value is a predictor of patient OS (P<0.001). The area under the time-dependent ROC curve (AUC) indicated that our signature had a relatively high true positive rate when predicting the 1-year, 3-year, and 5-year OS of the TCGA cohort, which was 0.735, 0.711, and 0.601, respectively. The analysis of the nomogram and calibration curve showed the predictive ability of the gene model. In addition, immune-related pathways were highlighted in the functional enrichment analysis, and immune response between the two risk groups was observed to be significantly different. All of the results proved the reliability of our iron metabolism-related gene risk prognostic model. Conclusion: We developed and verified a 12-gene prognostic signature, which can help predict the prognosis of lung adenocarcinoma and offer a variety of targeted options for the precise treatment of lung cancer.
Substituent-induced electronic localization of nickel phthalocyanine with enhanced electrocatalytic CO2 reduction
Authors: Chen Kejun , Cao Maoqi , Lin Yiyang , Fu Junwei , Liao Hanxiao , Xie Feng , Zhou Yajiao , Yang Baopeng , Liu Kang , Chen Guozhu , Li HongMei , Wu Dawang , Qiu Xiaoqing , Hu Junhua , Zheng Xu-Sheng , Shakouri Mohsen , Xiao Qunfeng , Hu Yongfeng , Li Jun , Liu Min 
Published: January 2021

Abstract
Designing efficient catalysts with high activity and selectivity is desirable and challenging for CO2 reduction reaction (CO2RR). Nickel phthalocyanine (NiPc) is a promising molecule catalyst for CO2RR. However, the pristine NiPc suffers from poor CO2 adsorption and activation due to its electron deficiency of Ni–N4 site, which leads to inferior activity and stability during CO2RR. Here, we develop a substituent-induced electronic localization strategy to improve CO2 adsorption and activation, and thus catalytic performance. Theoretic calculations and experimental results indicate that the electronic localization on the Ni site induced by electron-donating substituents (hydroxyl or amino) of NiPc greatly enhances the CO2 adsorption and activation, which is positively associated with the electron-donating abilities of substituents. Employing the optimal catalyst of amino-substituted NiPc to catalyze CO2 into CO in flow cell can achieve an ultrahigh activity and selectivity of 99.8% at the current densities up to 400 mA cm-2. This work offers a novel strategy to regulate the electronic structure of the active site by introducing substituents for highly efficient CO2RR.
Prospects of Shotcrete Shaft Lining for Underground Sustainable Mining
Authors: Samanta Binay Kumar , Singh Upendra Kumar 
Published: January 2021

Abstract
Investment in underground mining is coming down all over the world, but deep reserves are available for extraction. Conventional sinking and lining of mine shaft projets or pits for winding men and materials from deep underground mines are getting very costly and being deferred. We found Polish technology of Shaft sinking with monolithic mining very efficient in Sudamdih, Monidih and Satgram projects in India. Russian collaboration for Jhanjra for shaft sinking and mine construction for large mine has been successful. Underground production is declining world over, while remaining reserves are at greater depths. Designing and coding original model programs by collecting actual field data to run the programs to determine cost benefit at different depths with standard diameters has been done. Most companies are avoiding deep mining projects, because of exorbitant cost of shaft sinking. As per experience of the researcher, pre-split blasting and shotcrete lining can be much faster and cheaper. So model programs have been designed and run with practical field cost and technical data, as exemplified in this paper. Future of deep pit mining will be assured with successful adoption of the method in projects, especially through hard rock strata
Disruption of gastric mucous granule exocytosis by Helicobacter pylori virulence factor CagA
Authors: Ninomiya Ryo , Kubo Shuichi , Kajiwara Tooru , Koizumi Hiroko , Tokunaga Akinori , Uchida Tomohisa , Nabeka Hiroaki , Doihara Takuya , Li Cheng , Shimokawa Tetsuya , Matsuda Seiji , Kobayashi Naoto , Murakami Kazunari , Aigaki Toshiro , Fukuda Mitsunori , yamaoka Yoshio , Hamada Fumihiko 
Published: January 2021

Abstract
Helicobacter pylori infection is the strongest known risk factor of stomach cancer. Strains harboring the virulence factor CagA (cytotoxin-associated gene A) significantly stimulate host inflammatory response, which increases the risk of ulceration and cancer. However, the mechanisms by which CagA triggers prolonged inflammation with mucosal damage remain elusive. Based on a large-scale genetic screen using Drosophila, we identified a novel CagA target Synaptotagmin-like protein 2-a, Slp2-a, an effector of small GTPase Rab27. Using gastric organoid-derived monolayers of polarized mucous cells, we demonstrated that CagA inhibited Slp2-a-mediated docking of mucous granules to the plasma membrane by direct binding to Slp2-a. We further observed aberrant cytoplasmic retention of mucus in human gastric mucosa infected with CagA-expressing strains. These results suggest that CagA could be disrupting the protective mucous barrier by inhibiting Slp2-a-mediated mucous granule exocytosis, which may lead to mucosal damage from luminal acid and pepsin to promote inflammation leading to cancer.
Metabolomic Profiling of Brain Protective Effect of Edaravine on Cerebral Ischemia-reperfusion Injury in Mice
Authors: Ma Hui-fen , Su Lin-jie , Zhang Da-wei , Gong Shuai-shuai , Liu Yi-ning , Zhang Yuan-yuan , Li Fang , Kou Junping 
Published: January 2021

Abstract
Edaravine (EDA) injection has seen extensively applied in clinic for treating stroke. Nevertheless, the metabolite signatures and underlying mechanisms associated with EDA remains unclear, which deserves further elucidation for improving the accuracy of EDA usage. Ischemia stroke was simulated by intraluminal occlusion of the right middle cerebral artery for 1 h followed by reperfusion for 24 h in mice. Brain infarct size, neurogical deficits and lactate dehydrogenase (LDH) level were improved by EDA. Significantly differential metabolites were screened with untargeted metabolomics by cross-comparisons with pre- and post-treatment of EDA under cerebral ischemia/reperfusion (I/R) injury. The possibly involved pathways, such as valine, leucine and isoleucine biosynthesis, phenylalanine metabolism, taurine and hypotaurine metabolism, were enriched with differential metabolites and relatively regulatory enzymes, respectively. The network of differential metabolites was constructed for integral exhibition of metabolic characteristics. Targeted analysis of taurine, an important metabolic marker, was performed for further validating. The decreased level of taurine was confirmed in MCAO/R mice and increased by EDA. The inhibition of EDA on cerebral endothelial cell apoptosis was confirmed by TdT-mediated dUTP nick-end labeling (TUNEL) stain. Cysteine sulfinic acid decarboxylase (CSAD), the rate-limiting enzyme of taurine generation, significantly increased along with inhibiting endothelial cells apoptosis after treatment of EDA. Thus, CSAD, as the possible new therapeutic target of EDA was selected and validated by western blotting and immunofluorescence. Together, this study provided the metabolite signatures and identified CSAD as an unrecognized therapeutic intervention for EDA in treatment of ischaemic stroke via inhibiting brain endothelial cells apoptosis.
Antibiotics-induced Intestinal Dysbacteriosis Caused Behavioral Alternations and Neuronal Activation in Different Brain Regions in Mice
Authors: Wang Pan , Tu Ke , Cao Peng , Yang Yue-Fan , Zhang Hao , Qiu Xin-Tong , Zhang Ming-Ming , Wu Xiao-Jun , Yang Hui , Chen Tao 
Published: January 2021

Abstract
Antibiotics affect gut microbial composition, leading to Gut-Brain-Axis imbalance and neurobehavioral changes. However, the intestinal dysbacteriosis associated behavior changes are not consistently reported. It is not clear whether these changes are transient or permanent. The neuroprotective effect of probiotics against intestinal dysbacteriosis induced alternations needs to be determined either. In the present study, oral antibiotic mixture including Ampicillin, Streptomycin, and Clindamycin was utilized to induce intestinal dysbacteriosis in mice. Antibiotics application triggered mechanical allodynia in von frey test and spontaneous pain in open field test. It also resulted in increased anxiety and depressive-like behaviors and damaged spatial memory performance. After application of probiotics, the mechanical allodynia and spontaneous pain were alleviated significantly. The anxiety behaviors, depressive-like behaviors and recognitive performance were ameliorative as well. By using Fos protein as a marker, it is found that the sensory, emotion and memory related brain regions were activated in mice with intestinal dysbacteriosis. Our study is not only helpful for enriching our basic knowledge for understanding the changed pain responses and related brain disorders in antibiotics-induced dysbacteriosis mice, but also beneficial for providing a more comprehensive mechanistic explanation for the regulation of antibiotics and probiotics on gut microbiota and relevant alternations in animal neurological behaviors.
Mystery of Reversible Cerebral Vasoconstriction Syndrome (RCVS): An Interesting Case Report
Authors: Togha Mansoureh , Babaei Mahsa , Ghelichi Parvin Ganji 
Published: January 2021

Abstract
BackgroundReversible Cerebral Vasoconstriction Syndrome is a condition of transient cerebral vascular spasms presenting with recurrent thunderclap headaches and recovery within three months. Several probable triggers and underlying factors, such as sex hormones, vasoactive drugs, head trauma or surgery, and tumors, have been implicated.Case presentationIn this paper, we present a 53-year-old woman with severe thunderclap headaches and normal lab tests who was radio-clinically diagnosed with reversible cerebral vasoconstriction syndrome and treated accordingly. Then, she experienced the recurrence of RCVS after about 2 years and headaches after one year in association with high blood pressure, high blood sugar, hypothyroidism, hyperlipidemia, and a urine metanephrine level of 5 times higher than the normal limit, suggesting a diagnosis of pheochromocytoma. After confirmation of the diagnosis with further imaging studies, surgical removal of the tumor resolved all the signs and symptoms. ConclusionOften underdiagnosed, pheochromocytoma could be an important condition associated with RCVS. It is important for clinicians to bear this diagnosis in mind while dealing with similar cases of recurrent thunderclap headaches.
Transcriptomic Analysis of Fertilization Effects on the Biosynthesis of Sesquiterpenes in Essential Oils from Phoebe Bournei Twigs and Leaves
Authors: Liu Li , He Gongxiu , Wang Xu , Zhang Dangquan 
Published: January 2021

Abstract
Background: Phoebe bournei is a potential medicinal plant whose essential oil (EO) from leaves has potential inhibitory activities against some bacterium, tumor, and has a certain potential for hypoglycemic activity. Fertilization is a common and effective method to increase plant biomass, which can increase the raw material of essential oil, but has a certain impact on the composition and biological activity of plant essential oil. Results: The main components are sesquiterpenes in the essential oils from leaves and twigs. The yield of the essential oils and the content of their main components can be modulated by compost and compound fertilizer, to different degrees, and minor differences were registered among the categories of the components in essential oils. However, changes were strongly mirrored in some main components of essential oils. The content of the primary (+) - calarene in the leaf EO were strongly increased by compost, but the opposite happened by compound fertilizer. On the contrary, the effect of compound fertilizer was more significant on the main components of twig essential oil than compost. The transcriptome sequencing results of P. bournei showed that the total number of DEGs in twigs and leaves treated with compost were significantly more than that with compound fertilizer. No change was found in the expression of genes regulating principal components. However, the expression of several key genes regulating the upstream substrates for the synthesis of the sesquiterpenes was significantly changed: the expression of two key speed limiting enzymes genes (DXS and HMGR) and two important branch-point enzyme genes (FPPS and GGPPS) was significantly down regulated, while the expression of gene (HMGS) was significantly up-regulated.Conclusion: The expression levels of genes (DXS2, HMGR, FPPS and GGPPS) were significantly down regulated in leaves treated with compost, resulting in the changes of the yield and main components of the leaf essential oil. The effect of compost was more significant on the synthesis of the essential oil from P. bournei leaves than that of compound fertilizer.