bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences Between Effectiveness Of Inmas Acupuncture And Local Points In Back And Shoulder Pain
Authors: Purwanto Purwanto , Wahyu Jatmiko Rinto 
Published: May 2021

Background: Shoulder pain and low back pain usually arise because of overuse or excessive use. Acupuncture can relax back muscles, waist and shoulders through cutaneosomatic reflex; and increase the release of endorphins so that it can reduce inflammation in back, waist, and shoulders so that it can help relieve pain and restore the function of back, waist, and shoulders. This study aims to determine differences betwen effectiveness of INMAS and local points acupuncture on pain and quality of life in workers with back and shoulder pain.
Methods: This study is quantitative quasi experimental design with control group with pre and post test, conducted in July-September 2020 at Verta Medika Acupuncture, Klaten. The population was all patients with shoulder and back pain who underwent acupuncture therapy and the sample was determined by inclusion and exclusion criteria and divided into two groups using simple random sampling. Each group consisted of 15 patients. Data analysis focused on pain scale (NRS) and quality of life (WHO-QoL).
Results: INMAS technique was better at reducing pain scale (ρ = 0.001), quality of physical health (ρ = 0.083), quality of psychological health (ρ = 0,000), quality of social (ρ = 0,000), and quality of environmental health (ρ = 0.045).
Conclusion: INMAS technique is more effective than local points acupuncture for reducing pain levels and it improves the quality of life. Research using larger population and sample is needed to increase validity and reliability of the study.
Implementasi Sistem Pakar dengan Algortima Naïve Bayes dengan Laplace Correction untuk Diagnosis Tuberkulosis Paru
Authors: Yanti Apriyani  , Iskandar Iqbal Dzulfiqar Iskandar , Mira Kusmira  , Melisa Winda Pertiwi  , Imam Amirulloh  , Taufik Wibisono  
Published: May 2021

Tuberkulosis atau tuberculosis (TBC) merupakan salah satu penyakit menular berbahaya yang disebabkan oleh kuman dari kelompok Mycobacterium Tuberculosis. Sebagian besar kuman tuberkulosis menyerang organ paru-paru dan menyerang organ yang lainnya. Menurut WHO Indonesia menjadi salah satu negara dengan beban tuberkulosis tinggi dengan jumlah kasus terbaru Tuberkulosis di Indonesia mencapai 420.994 kasus pada tahun 2017, hal tersebut dipengaruhi oleh kurangnya pengetahuan masyarakat akan tuberculosis dan enggan untuk memeriksakan gejala-gejala awal yang diderita kepada dokter.
Dari permasalahan tersebut perlu dibangun sebuah sistem pakar yang berbasis perangkat lunak bertujuan untuk membantu dan memudahkan masyarakat dalam mendiagnosis gejala awal tuberculosis sebelum berkonsultasi kepada tenaga Kesehatan lebih lanjut. Diagnosis penyakit dengan menggunakan sistem pakar memerlukan sebuah metode algoritma dalam penyelesaiannya. Pada riset ini metode yang digunakan untuk membangun perangkat lunak sistem pakar diagnosis tuberculosis paru menggunakan waterfall, sedangakan proses klasifikasi untuk menentukan Diagnosis menggunakan algortima Naïve Bayes dengan Laplace Correction. Tujuan dari riset ini adalah untuk mendiagnosis gejala-gejala yang dialami pasien dan dapat menyimpulkan layaknya seorang pakar atau dokter saat mendiagnosis pasiennya serta memberikan pengetahuan gangguan apa yang diderita oleh pasien.
Terkait hasil riset ini adalah berupa aplikasi sistem pakar Diagnosis penyakit tuberculosis paru berbasis desktop, dan telah dilakukan Pengujian sistem. Pengujian sistem ini dilakukan menggunakan black box testing yang berfokus pada proses masukan dan keluaran dari aplikasi terhadap perangkat lunak Diagnosis penyakit tuberkulosis. Sedangkan data hasil uji aplikasi Diagnosis menunjukan Kelas Positif Tuberkulosis lebih besar dengan nilai 0.000129803, dibandingkan dengan nilai kelas Negatif Tuberkulosis dengan nilai 0.0000000018. Maka dapat dinyatakan bahwa pasien tersebut Positif Tuberkulosis atau Pasien Mengalami Tuberkulosis Paru. Dampak dari riset ini masyarakat dapat mendiagnosis secara mandiri sehingga dapat mengetahui gejala awal dari tuberculosis.
Examining Ghana’s Cash Transfer Programme Outcomes in the Ejisu-Juaben Municipality from Conversation and Membership Categorisation Analyses Perspectives
Authors: Puorideme Dennis 
Published: May 2021

Since the start of cash transfer programmes in developing countries in the late 1990s and its spread, studies have demonstrated a variety of outcomes comprising education, health, and nutrition for the poorest households. These studies focused on macro analysis of programmes’ outcomes but paid little attention to an indepth micro study of the everyday intersubjective accounts and actions of local community focal persons and caregivers, which construct programme outcomes. The objective of this study is to highlight the everyday concrete outcomes of a cash transfer programme in Ejisu-Juaben Municipality in Ghana. This study draws on Foucault’s notion of subjectivation and discourse to construct a conversation and membership categorisation analyses framework to explore community focal persons’ and female caregivers’ conversations from focus group discussions. The Livelihood Empowerment against Poverty cash transfer programme in Ghana is the empirical case. This article demonstrates that caregivers and poor households arehappier, practice joint decision-making, and have cohesive social relations in poor households. Thus, localised programme outcomes improved participation in the decision-making, happiness, and social cohesion of beneficiary poor households. Evaluation mechanisms for programme outcomes could consider the everyday intersubjective accounts, practices of focal persons, caregivers/beneficiaries in poor households at the micro-level.
Keywords: Social Protection, Ethnography, Discourse, Subjectivation, Governmentality
Optimized models of xenobiotic-induced oxidative stress in HepG2 cells
Authors: Sriset Yollada , Chatuphonprasert Waranya , Jarukamjorn Kanokwan 
Published: May 2021

Purpose: To evaluate the molecular impact of ethanol, sodium selenite, and tert-butyl hydroperoxide (TBHP) on oxidant-antioxidant balance in HepG2 cells to establish an optimized oxidative stress model of HepG2 cells.
Methods: HepG2 cells were treated with ethanol (10 - 500 mM) and sodium selenite (1 - 10 µM) for 24 and 48 h and with TBHP (50 - 200 µM) for 3 and 24 h, respectively. Biomarkers for cellular injury, ie, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and for antioxidant system, i.e., superoxide dismutase (SOD), catalase (CAT), and total glutathione content, were determined.
Results: All treatments increased the levels of LDH, AST, ALT, and MDA but decreased SOD and CAT activities and the total glutathione content in HepG2 cells. Oxidative stress was induced by these oxidative stressors in HepG2 cells via oxidant-antioxidant imbalance, with TBHP (100 µM, 3 h) acting as a powerful oxidant based on the minimal time to induce oxidative stress. The antioxidants, ascorbic acid and gallic acid, improved oxidant-antioxidant imbalance against xenobiotic-induced oxidative stress in HepG2 cells.
Conclusion: These oxidative stress models are suitable for investigating the antioxidant and/or hepatoprotective potential of chemicals, including natural compounds.
Effect of solvent fractions of crude extract of Liushenqu on gastrointestinal motility in guinea pigs, and the underlying mechanism(s)
Authors: Zhang Hongling , Zeng Hairong , Wang Ling , Qin Yi , Huang Qinwan , Sun Jilin , Yao Renchuan 
Published: May 2021

Purpose: To study the effect of solvent fractions of the crude extract of liushenqu on gastrointestinal motility in guinea pigs, and the mechanism of action.
Methods: The effects of solvent fractions of crude extract of liushenqu (LSQ) on receptors in guinea pig isolated small intestinal cells were determined by treatment with different receptor blockers, including diphenhydramine (0.067 mg/mL), atropine sulfate (0.064 mg/mL), propranolol hydrochloride (0.033mg/mL), phentolamine mesylate (0.04mg/mL) and ondansetron hydrochloride (0.048mg/mL), to investigate the possible pharmacological mechanism of action.
Results: There was no significant change in the maximum amplitude of muscle tension before and after administration in the control group, petroleum ether fraction group, and dichlormethane fraction group, while muscle tension in the 95 % ethanol and n-butanol fractions significantly increased (p < 0.01). The mean changes in tension were significantly different from that of control group (p < 0.01), but ethyl acetate fraction showed significant intestinal muscle inhibition (p < 0.01). Addition of LSQ did not alleviate the inhibition caused by diphenhydramine, but it significantly reversed the inhibition caused by blockers of cholinergic muscarinic receptor, adrenergic alpha- and beta- receptors, and 5-HT receptor (p < 0.01).
Conclusion: These results indicate that n-butanol fraction is the most effective bioactive fraction of LSQ, while ethyl acetate fraction has the opposite effect. In addition, its mechanism of action is related to increase in the amplitude of small intestine smooth muscle contraction and acceleration of small intestine peristalsis.
Biological screening and docking studies of unique hybrids synthesized by conventional versus microwave assisted techniques
Authors: Virk N.A. , Aziz-ur-Rehman  , Abbasi M.A. , Siddiqui S.Z. , Ashraf A. , Iqbal J. , Rasoo S. , Khalid H. , Laulloo S.J. , Khan S.U. , Shah S.A.A. 
Published: May 2021

Purpose: To carry out the synthesis of various hybrids of 1,2,4-triazole in search of potential therapeutic enzyme inhibitory agents, and carry out docking and bovine serum albumin (BSA) binding studies on docking and bovine serum albumin (BSA) binding studies on the hybrids.
Methods: The target compounds were synthesized by following a multistep protocol. Compound 1 was synthesized from 4-methoxybenzenesulfonyl chloride (a) and ethyl isonipecotate (b). Compound 1 was refluxed with hydrazine to synthesize compound 2, which was converted to compound 3 through two consecutive steps. Compound 4 and different amines (5a-5i), were utilized to synthesize an array of electrophiles (6a-6i). A series of 1,2,4-triazole hybrids (7a-7i) were synthesized at room temperature by stirring together 3 and 6a-6i. The final structures of 7a-7i were elucidated through 1H-NMR, 13C-NMR and EI-MS spectroscopy. The BSA binding studies were performed by fluorometric titration. Furthermore, antioxidant and enzyme inhibition activities were determined colorimetrically.
Results: Compound 7d was the most active antioxidant agent, compared to butylated hydroxyanisole (BHA), while compounds 7d, 7e, 7f, 7g and 7i proved to be potent urease inhibitors with half-maximal inhibitory concentration (IC50) values of 19.5 ± 0.12, 21.1 ± 0.68, 18.2 ± 0.78, 19.9 ± 0.77 and 17.9 ± 0.10 µM, respectively, compared to thiourea with an IC50 of 24.3 ± 0.24 µM. Compounds 7a, 7b, 7d, and 7e exhibited high butyrylcholinesterase inhibition potential, compared to eserine.
Conclusion: The synthesized compounds require studies further as potential therapeutic enzyme inhibitory agents in view of their urease inhibition as well as antioxidant activity.
Synergistic effect of radiotherapy and dendritic-cell-based immunotherapy via miR-15b-5p regulation in a colon carcinoma mouse model
Authors: Zhang Xiaolian , Wei Wei , Xia LiHong , Zheng Ruzhen 
Published: May 2021

Purpose: To determine the combined effect of radiotherapy (RT) and dendritic cell (DC)-based immunotherapy on tumor regression, and the influence of miR-15b-5p on the sensitivity of the tumor to RT/DC.
Methods: Colon carcinoma cell line (T-26) was injected subcutaneously into BALB/c mice to generate xenograft tumors. The animals were exposed to radiation therapy (RT) only or DC only, or RT plus DC (RT/DC). Tumor progression was measured and analyzed followed by quantification of miR-15b-5p using qPCR in each group 21 days after tumor inoculation. Overexpressed xenograft tumors were generated by transfecting MiR-15b-5p mimic to CT-26 cell lines and subcutaneously administered to mice. The mice were then subjected to RT/DC treatment and assessed for tumor growth, TUNEL staining, as well as mRNA and protein expressions of B-cell lymphoma-2 (BCL-2), caspase-3 (casp-3), and X-linked inhibitor of apoptosis protein (XIAP).
Results: RT/DC combination treatment resulted in tumor regression and increased miR-15b-5p expression, when compared with other groups treated with RT and DC alone (p < 0.001). There was a marked reduction in tumor growth following RT/DC combination therapy in miR-15b-5p overexpressed xenograft tumor (p < 0.001). Moreover, miR-15b-5p/(RT/DC) treatment significantly increased cancer cell apoptosis. These results were substantiated by increased mRNA and protein expressions of casp-3, reduced BCL-2 and XIAP (p < 0.001).
Conclusion: These findings demonstrate that RT/DC combination therapy increases miR-15b-5p levels in mice, and that miR-15b-5p upregulation enhances the sensitivity of tumors to RT/DC combination therapy. Therefore, the combination of RT and dendritic cell-based immunotherapy offers significant therapeutic advantage in controlling cancers, including metastatic tumors.
Peristiwa Ketidakpastian Pada Pelaksanaan Tol Layang A.P.Pettarani Seksi III Kota Makassar
Authors: Tambunan Rebecca S , Sopacua Helen A.I. , Latupeirissa Josefine E 
Published: May 2021

Jalan Tol Layang A. P. Pettarani merupakan proyek kontruksi yang memakan waktu cukup lama dan kompleks sehingga dapat menimbulkan ketidakpastian yang akhirnya akan memunculkan berbagai macam risiko. Dampak risiko tersebut dapat mempengaruhi produktivitas, kinerja/performance, kualitas dan anggaran biaya proyek. Kesuksesan proyek konstruksi sangat tergantung dari kemampuan manager proyek dalam mengelola risiko yang terjadi. Manajemen risiko meliputi langkah- langkah yang terkait usaha pelaksanaan perencanaan manajemen risiko, identifikasi, tanggapan, dan monitoring serta pengawasan pada suatu proyek. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengidentifikasi, menganalisis, serta mengetahui respon risiko-risiko yang dominan terjadi, dengan menggunakan metode pengumpulan data primer berupa kuisioner dengan menggunakan skala likert, matriks Probability x Impact, Risk Map dan wawancara, dimana pada penelitian ini didapatkan sebelas variabel risiko yang dominan terhadap proyek ini.
Evaluation and Insilico Analysis of Genes Involved in Hypothyroidism
Authors: Masood Ammara , Mubeen Hira , Iqbal Nadia 
Published: May 2021

To evaluate the expression of genes that involved in hypothyroidism by multiple alignment of nucleotide sequences of genes study the functional unit of genes that involve in production of thyroid hormones. The thyroid gland is part of endocrine gland and regulates much vital body function (breathing, heart rate, body weight, body temperature also involve in development, growth, protein production and it also control the central and peripheral nervous system, muscle strength and menstrual cycle). Hypothyroidism is a condition in which thyroid gland does not produce enough thyroid hormone. To find the genetics information than by using bioinformatics tools several genes that were involved in hypothyroidism were identified but six genes TSHR, TPO, THRB, TG, IYD, DUOX2 and their motifs were studied in detail to understand the basic domains involved in this disease.
Synergistic Effect of the Combination of Commercial Essential Oils with Standard Antibiotics: In vitro Evaluation
Authors: Mehboob Aneela , Abbas Tanveer 
Published: May 2021

The aim of this investigation was to determine the antibacterial activity of essential oils and to assess the outcomes produced by the combinations of antibiotics and essential oils. To execute this research, gold standard and conventional methods were used. Antibacterial potency of five essential oils namely Citrus limon, Elettaria cardamomum, Lavandula angustifolia, Nigella sativa and Prunus dulcis were tested against Escherichia coli, Serratia fonticola, Serratia liquefaciens, Citrobacter freundii and Staphylococcus aureus recouped from street foods of Karachi. Among five of them, Citrus limon and Lavandula angustifolia were the most potent essential oils showing highest antibacterial activity in their undiluted form with the exception of Staphylococcus aureus but their mix at different concentrations successfully inhibited the growth of Staphylococcus aureus and Serratia fonticola. Synergistic outcomes were achieved against all the tested bacterial strains from the mix of essential oils and antibiotics, however antagonistic results were also obtained. This exploration underpins the application of essential oils alone and in combinations with antimicrobial agents to improve the affectability of ineffective drugs and aides in the advancement of new antimicrobial drugs to treat bacterial infections utilizing therapeutic plants.
Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii
Authors: Yoneda Kazunari , Sakuraba Haruhiko , Araki Tomohiro , Ohshima Toshihisa 
Published: May 2021

In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed 1H‐NMR spectroscopy using deuterium‐labeled NADH (4R‐2H‐NADH) and molecular docking simulations to reveal that the pro‐S hydrogen at the C4 position of the nicotinamide moiety in NADH was specifically transferred to the flavin‐N5 atom of FNM. Altogether, our findings may aid in the improvement of the indigo dyeing (Aizome) process.
Fabrication of CuO/TiO2/Ti Monolithic Catalyst for Efficient and Stable CO Oxidation
Authors: Tang Xinyue , Wang Junchao , Liu Xiaojue , Yang Liu , Liu Baodan , Jiang Xin 
Published: May 2021

AbstractThis work reports a novel strategy to in situ grow TiO2 nanosheets on flexible Ti mesh as ideal support, and to modify CuO active components through multi‐step methods. The CuO/TiO2/Ti monolithic catalyst can realize the complete CO oxidation at 110 °C without the assistance of any noble metal and the outstanding long‐term stability can be also achieved. When using urea as deposition precipitant, CuO particle size will increase obviously with the reaction time longer than 6 h, which is adverse to the catalytic performance, showing the significant impact of CuO particle size on the CO oxidation capability. The deposition reaction carried out in different solutions is also investigated and the results show that CuO/TiO2/Ti catalysts synthesized in acid and alkaline condition exhibit superior catalytic performance owing to the high content of Cu1+/0 and adsorbed oxygen species (Oα) on surface, large surface area, and excellent redox capacity compared with that in reductive solution. More importantly, the as‐synthesized CuO/TiO2/Ti monolithic catalysts show outstanding low‐temperature performance and excellent long‐term stability, indicating the overwhelming advantage of in situ grown catalysts. It is expected that the peculiar in situ growth technology is capable of extending to monolithic catalysts for multiple applications in the future.
A Practical Synthetic Route to Artepillin C and Drupanin
Authors: Zhao Xue‐Zheng , Wang Lu , Li Xiao‐Ya , Zhang Qing‐Qing , Li Wei , Lin Ai‐Jun , Xu Jin‐Yi , Wu Xiao‐Ming , Xie Wei‐Jia 
Published: May 2021

AbstractSynthesis of Artepillin C, a natural product with diverse biological activities, as well as Drupanin were accomplished. A two‐step continuous CuCN‐meditated prenylation of protected 3,5‐dibromo‐4‐hydroxybenzaldehyde, followed by HWE reaction efficiently provided desired diprenylated p‐cinnamic acid backbone structure.
State‐of‐the‐art of lumbar puncture and its place in the journey of patients with Alzheimer's disease
Authors: Hampel Harald , Shaw Leslie M. , Aisen Paul , Chen Christopher , Lleó Alberto , Iwatsubo Takeshi , Iwata Atsushi , Yamada Masahito , Ikeuchi Takeshi , Jia Jianping , Wang Huali , Teunissen Charlotte E. , Peskind Elaine , Blennow Kaj , Cummings Jeffrey , Vergallo Andrea 
Published: May 2021

AbstractRecent advances in developing disease‐modifying therapies (DMT) for Alzheimer's disease (AD), and the recognition that AD pathophysiology emerges decades before clinical symptoms, necessitate a paradigm shift of health‐care systems toward biomarker‐guided early detection, diagnosis, and therapeutic decision‐making. Appropriate incorporation of cerebrospinal fluid biomarker analysis in clinical practice is an essential step toward system readiness for accommodating the demand of AD diagnosis and proper use of DMTs—once they become available. However, the use of lumbar puncture (LP) in individuals with suspected neurodegenerative diseases such as AD is inconsistent, and the perception of its utility and safety differs considerably among medical specialties as well as among regions and countries. This review describes the state‐of‐the‐art evidence concerning the safety profile of LP in older adults, discusses the risk factors for LP‐associated adverse events, and provides recommendations and an outlook for optimized use and global implementation of LP in individuals with suspected AD.
Undiagnosed tracheomalacia accompanied with accidental expiratory central airway collapse after tracheal intubation
Authors: Sonoda Seijiro , Sato Kozo , Takagi Yoshito , Sato Yumiko , Murao Fumi , Koide Yasuhiro , Oda Toshiyuki 
Published: May 2021

BackgroundA patient with undiagnosed tracheomalacia undergoing surgery experienced accidental expiratory central airway collapse after tracheal intubation. Here, we aimed to diagnose tracheomalacia from the preoperative data.Case PresentationA 73‐year‐old man, scheduled for abdominal surgery, had a clinical history of chronic obstructive pulmonary disease. Preoperative chest computed tomography revealed a lateral narrowing of the tracheal shape. After tracheal intubation, we could not manually ventilate the inflated lung. Emergent bronchoscopy findings, including severe expiratory tracheal collapse, indicated a diagnosis of tracheomalacia. We could fully ventilate the patient by moving the endotracheal tube near the tracheal carina and finally changing it to a double‐lumen tube. Airway collapse did not occur under spontaneous breathing.ConclusionAccidental expiratory central airway collapse could occur in patients with undiagnosed tracheomalacia during surgery. A diagnosis of tracheomalacia should be presumed from a deformed trachea on preoperative imaging and history of chronic obstructive pulmonary disease.
Coupling Aptamer‐based Protein Tagging with Metabolic Glycan Labeling for In Situ Visualization and Biological Function Study of Exosomal Protein‐Specific Glycosylation
Authors: Zhu Lin , Xu Yuanfeng , Wei Xinyu , Lin Haoting , Huang Mengjiao , Lin Bingqian , Song Yanling , Yang Chaoyong 
Published: May 2021

AbstractExosomal glycoproteins play important roles in many physiological and pathological functions. Herein, we developed a dual labeling strategy based on a protein‐specific aptamer tagging and metabolic glycan labeling for visualizing glycosylation of specific proteins on exosomes. The glycosylation of exosomal PD‐L1 (exoPD‐L1) was imaged in situ using intramolecular fluorescence resonance energy transfer (FRET) between fluorescent PD‐L1 aptamers bound on exoPD‐L1 and fluorescent tags on glycans introduced via metabolic glycan labeling. This method enables in situ visualization and biological function study of exosomal protein glycosylation. Exosomal PD‐L1 glycosylation was confirmed to be required in interaction with PD‐1 and participated in inhibiting of CD8+ T cell proliferation. This is an efficient and non‐destructive method to study the presence and function of exosomal protein‐specific glycosylation in situ, which provides a powerful tool for exosomal glycoproteomics research.