bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electroencephalographic assessment of infant spinal anesthesia: A pilot prospective observational study
Authors: Whitaker Emmett E. , Chao Jerry Y. , Holmes Gregory L. , Legatt Alan D. , Yozawitz Elissa G. , Purdon Patrick L. , Shinnar Shlomo , Williams Robert K. 
Published: September 2021

AbstractIntroductionSpinal anesthesia is utilized as an alternative to general anesthesia in infants for some surgeries. After spinal anesthesia, infants often become less conscious without administration of sedative medications. The aim of this study was to assess electroencephalographic (EEG) correlates after spinal anesthesia in a cohort of infants.Patients and MethodsThis pilot study included 12 infants who underwent spinal anesthesia. Unprocessed electroencephalography was recorded. The electroencephalogram was interpreted by four neurologists. Processed analyses compared electroencephalogram changes 30 min after spinal anesthesia to baseline.ResultsFollowing spinal anesthesia, all 12 infants became sedated. Electroencephalography in all 12 demonstrated Stage 2 sleep with the appearance of sleep spindles (12–14 Hz) in the frontal and central leads in 8/12 (67%) of subjects. The median time to onset of sleep spindles was 24.7 interquartile range (21.2, 29.9) min. The duration of sleep spindles was 25.1 interquartile range (5.8, 99.8) min. Voltage attenuation and background slowing were the most common initial changes. Compared to baseline, the electroencephalogram 30 min after spinal anesthesia showed significantly increased absolute delta power (p = 0.02) and gamma power (p < 0.0001); decreases in beta (p = 0.0006) and higher beta (p < 0.0001) were also observed. The Fast Fourier Transform power ratio difference for delta/beta was increased (p = 0.03). Increased coherence was noted in the delta (p = 0.02) and theta (p = 0.04) bandwidths.DiscussionSpinal anesthesia in infants is associated with increased electroencephalographic slow wave activity and decreased beta activity compared to the awake state, with appearance of sleep spindles suggestive of normal sleep. The etiology and significance of the observed voltage attenuation and background slowing remains unclear.ConclusionsThe EEG signature of infant spinal anesthesia is distinct from that seen with general anesthesia and is consistent with normal sleep. Further investigation is required to better understand the etiology of these findings. Our preliminary findings contribute to the understanding of the brain effects of spinal anesthesia in early development.
Characterization of epidermal bladder cells in Chenopodium quinoa
Authors: Otterbach Sophie L. , Khoury Holly , Rupasinghe Thusitha , Mendis Himasha , Kwan Kim H. , Lui Veronica , Natera Siria H. A. , Klaiber Iris , Allen Nathaniel M. , Jarvis David E. , Tester Mark , Roessner Ute , Schmöckel Sandra M. 
Published: September 2021

AbstractChenopodium quinoa (quinoa) is considered a superfood with its favourable nutrient composition and being gluten free. Quinoa has high tolerance to abiotic stresses, such as salinity, water deficit (drought) and cold. The tolerance mechanisms are yet to be elucidated. Quinoa has epidermal bladder cells (EBCs) that densely cover the shoot surface, particularly the younger parts of the plant. Here, we report on the EBC's primary and secondary metabolomes, as well as the lipidome in control conditions and in response to abiotic stresses. EBCs were isolated from plants after cold, heat, high‐light, water deficit and salt treatments. We used untargeted gas chromatography–mass spectrometry (GC–MS) to analyse metabolites and untargeted and targeted liquid chromatography‐MS (LC–MS) for lipids and secondary metabolite analyses. We identified 64 primary metabolites, including sugars, organic acids and amino acids, 19 secondary metabolites, including phenolic compounds, betanin and saponins and 240 lipids categorized in five groups including glycerolipids and phospholipids. We found only few changes in the metabolic composition of EBCs in response to abiotic stresses; these were metabolites related with heat, cold and high‐light treatments but not salt stress. Na+ concentrations were low in EBCs with all treatments and approximately two orders of magnitude lower than K+ concentrations.
Risk aversion and elite‐group ignorance
Authors: Kinney David , Bright Liam Kofi 
Published: September 2021

AbstractCritical race theorists and standpoint epistemologists argue that agents who are members of dominant social groups are often in a state of ignorance about the extent of their social dominance, where this ignorance is explained by these agents' membership in a socially dominant group (e.g., Mills 2007). To illustrate this claim bluntly, it is argued: 1) that many white men do not know the extent of their social dominance, 2) that they remain ignorant as to the extent of their dominant social position even where this information is freely attainable, and 3) that this ignorance is due in part to the fact that they are white men. We argue that on Buchak's (2010, 2013) model of risk averse instrumental rationality, ignorance of one's privileges can be rational. This argument yields a new account of elite‐group ignorance, why it may occur, and how it might be alleviated.
Multi‐infections, competitive interactions, and pathogen coexistence
Authors: Dutt Agathe , Andrivon Didier , Le May Christophe 
Published: September 2021

AbstractMultiple infections, either simultaneous or sequential, affecting a single plant or crop are now recognized to be common in plant disease epidemics. These multiple infections thus generate a range of competitive interactions (exploitation competition, apparent competition, or interference competition) that directly impact competitors’ life history traits, and hence fitness. While a theoretical framework, based primarily on three ecological theories—niche exclusion, the “tragedy of the commons”, and the virulence–transmission trade‐off, provides insights into potential outcomes of multiple infections on pathogen evolution and virulence and on disease dynamics, experimental observations, although rather rare so far, strongly suggest that unexpected outputs can also occur. This review therefore attempts to provide a comprehensive overview of both theoretical and empirical knowledge about multiple infection in plants. It highlights the need for a detailed assessment of pathogen life history traits and their modulation according to competition types, host receptivity, and pathogen life strategies for a better understanding and prediction of the evolutionary and demographic outcomes of multiple infections, as well as research questions still open in this emerging field of plant pathology.
Monitoring of Brazilian wheat blast field populations reveals resistance to QoI, DMI, and SDHI fungicides
Authors: Vicentini Samara N. C. , Casado Priscila S. , de Carvalho Giselle , Moreira Silvino I. , Dorigan Adriano F. , Silva Tatiane C. , Silva Abimael G. , Custódio Adriano A. P. , Gomes Ana Carolina S. , Nunes Maciel João L. , Hawkins Nichola , McDonald Bruce A. , Fraaije Bart A. , Ceresini Paulo C. 
Published: September 2021

AbstractWheat blast is one of the most important and devastating fungal diseases of wheat in South America, South‐east Asia, and now in southern Africa. The disease can reduce grain yield by up to 70% and is best controlled using integrated disease management strategies. The difficulty in disease management is compounded by the lack of durable host resistance and the ineffectiveness of fungicide sprays. New succinate dehydrogenase inhibitor (SDHI) fungicides were recently introduced for the management of wheat diseases. Brazilian field populations of the wheat blast pathogen Pyricularia oryzae Triticum lineage (PoTl) sampled from different geographical regions in 2012 and 2018 were shown to be resistant to both QoI (strobilurin) and DMI (azole) fungicides. The main objective of the current study was to determine the SDHI baseline sensitivity in these populations. Moderate levels of SDHI resistance were detected in five out of the six field populations sampled in 2012 and in most of the strains isolated in 2018. No association was found between target site mutations in the sdhB, sdhC, and sdhD genes and the levels of SDHI resistance, indicating that a pre‐existing resistance mechanism not associated with target site mutations is probably present in Brazilian wheat blast populations.
Effects of UV radiation on transcript and metabolite accumulation are dependent on monochromatic light background in cucumber
Authors: Palma Carolina Falcato Fialho , Castro‐Alves Victor , Rosenqvist Eva , Ottosen Carl‐Otto , Strid Åke , Morales Luis Orlando 
Published: September 2021

AbstractDuring recent years, we have advanced our understanding of plant molecular responses to ultraviolet radiation (UV, 280–400 nm); however, how plants respond to UV radiation under different spectral light qualities is poorly understood. In this study, cucumber plants (Cucumis sativus “Lausanna RZ F1”) were grown under monochromatic blue, green, red, and broadband white light in combination with UV radiation. The effects of light quality and UV radiation on acclimatory responses were assessed by measuring transcript accumulation of ELONGATED HYPOCOTYL 5 (HY5), CHALCONE SYNTHASE 2 (CHS2), and LIGHT HARVESTING COMPLEX II (LHCII), and the accumulation of flavonoids and hydroxycinnamic acids in the leaves. The growth light backgrounds differentially regulated gene expression and metabolite accumulation. While HY5 and CHS2 transcripts were induced by blue and white light, LHCII was induced by white and red light. Furthermore, UV radiation antagonized the effects of blue, red, green, and white light on transcript accumulation in a gene‐dependent manner. Plants grown under blue light with supplementary UV radiation increased phenylalanine, flavonol disaccharide I and caffeic acid contents compared to those exposed only to blue light. UV radiation also induced the accumulation of flavonol disaccharide I and II, ferulic acid hexose and coumaric acid hexose in plants grown under green light. Our findings provide a further understanding of plant responses to UV radiation in combination with different light spectra and contribute to the design of light recipes for horticultural practices that aim to modify plant metabolism and ultimately improve crop quality.
Comparative transcriptome analysis of root types in salt tolerant and sensitive rice varieties in response to salinity stress
Authors: Cartagena Joyce A. , Yao Yao , Mitsuya Shiro , Tsuge Takashi 
Published: September 2021

AbstractSalinity tolerance in rice is a very important trait, especially in areas that are affected by soil salinity, such as tsunami‐devastated areas and coastal regions in rice‐producing countries. The roots are the key organs that first detect and respond to salinity stress; thus, it is important to have an understanding of how roots contribute to salinity tolerance in agricultural crops. After salinity treatment of the salt tolerant (Mulai) and sensitive (IR29) rice varieties, it appeared that among the three types of roots, the L‐type lateral roots (LLR) were the most sensitive to salinity stress in Mulai and the most tolerant in IR29. The nodal roots (NR) and the S‐type lateral roots (SLR) were all negatively affected by salinity treatment in both rice varieties. In order to elucidate the molecular mechanism of the difference in stress response among rice root types, the RNA‐seq transcriptome profiles of NR, LLR, and SLR were analyzed in Mulai and IR29. Between the two rice varieties, more transporters were found to participate in the regulation of salt tolerance in Mulai roots, such as those involved in ion and sugar transport. In IR29, many of the genes detected were associated with transcription regulation, including stress‐inducible genes such as NAC, WRKY and MYB. Among the different root types, gene expression in LLR and SLR were significantly regulated in both rice varieties. Taken together, the genes identified in this study may be utilized in the varietal improvement of rice with very specific root traits that can enhance tolerance to salinity stress.
Metabolomics in salmonid aquaculture research: Applications and future perspectives
Authors: Lulijwa Ronald , Alfaro Andrea C. , Young Tim 
Published: September 2021

AbstractSalmonids are the third major farmed finfish species after carps and tilapines, and thus contribute to global food fish production. However, as the aquaculture industry continues to grow, several challenges have emerged. Left unchecked, current problems will hinder aquaculture development. Metabolomics is one of the powerful biotechnological tools that will contribute to solving some of the pressing problems. This review aims to summarise findings and identify gaps from studies that used metabolomics in farmed salmon research. We extracted methodological information for comparison, highlighted analytical platform usages and identified most studied salmonids and sample types. We reviewed key articles to highlight the latest research themes. From the identified research gaps, future perspectives regarding potential use of metabolomics to solve issues in ecotoxicology, thermotolerance, nutrition, post‐harvest quality, health and disease, and husbandry practices are provided. The survey also highlighted improvements in execution of metabolomics protocols in aquaculture.
On the silver jubilee of crustacean antimicrobial peptides
Authors: Matos Gabriel Machado , Rosa Rafael Diego 
Published: September 2021

AbstractAntimicrobial peptides (AMPs) are widely distributed effector molecules that provide a first line of defence for organisms that lack adaptive immunity, such as crustaceans. They also represent bioactive molecules with great potential as therapeutic agents in aquaculture and biomedicine. Over the last years, an intensive research effort has been devoted to the identification and characterisation of antimicrobials in crustaceans resulting in the recognition of twelve gene‐encoded AMP families to date. In addition to classical linear and multi‐domain AMPs, crustaceans also rely on the production of unconventional ones, such as lysozymes and AMPs encrypted in large proteins carrying nonimmune functions. Besides their molecular and functional diversity, crustaceans have a particular AMP repertoire usually composed of diverse multigene families of taxonomically restricted peptides/polypeptides. In fact, only a few families of AMPs are shared between crustaceans and their close arthropod relatives. This review celebrates the silver jubilee of the discovery of the first crustacean AMPs and presents an updated and comprehensive overview in terms of structure, diversity and biological properties, emphasising their role in immune responses and in host‐microbe interactions. We also discuss the main progress achieved so far and major challenges remaining.
Clinical endometritis with Trueperella pyogenes reduces reproductive performance and milk production in dairy cows
Authors: Paiano Renan B. , Bonilla Jeannine , Moreno Andrea M. , Baruselli Pietro S. 
Published: September 2021

AbstractThe aim of this study was to identify the impact of Trueperella pyogenes in cows with clinical endometritis (CE) on reproductive performance and milk production in affected cows. In total, 230 lactating Holstein dairy cows from six commercial dairy herds were sampled once between 28 and 33 days post‐partum. Cows included in the present study did not receive antibiotic or anti‐inflammatory treatments prior to the experimental period. Clinical endometritis (CE) was characterized as cow with vaginal mucus score = 3 (>50% of purulent vaginal discharge) and >18% polymorphonuclear neutrophilic leukocyte (PMNL). The body condition scores (BCS) and milk production were evaluated at the time of enrolment. The identification of isolated bacteria was carried out through the analysis of MALDI‐TOF MS (matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry). According to uterine health, three groups of dairy cows were formed: healthy control cows without T. pyogenes (n = 147), CE cows with T. pyogenes (n = 22) and CE cows without T. pyogenes (n = 61). CE cows with T. pyogenes had lower BCS, milk production and conception at first AI (p < .01) than CE cows without T. pyogenes and control cows. Furthermore, CE cows with T. pyogenes had higher (p < .01) service per pregnancy and had greater (p < .01) days to get pregnant than CE cows without T. pyogenes and control cows. This study demonstrates that CE cows with T. pyogenes had impaired reproductive performance and milk production when compared to cows without CE and CE cows without T. pyogenes. This information can contribute to a strategic treatment in cows affected by clinical endometritis, favouring the rational use of antibiotics on dairy farms.
Feminist ethnography in the study of religion
Authors: Gillson Gwendolyn 
Published: September 2021

AbstractWhat does it mean to be a feminist ethnographer of religion? As a methodological, epistemological, and theoretical framework, feminist ethnography prioritizes participant observation that recognizes copresence, power, and reciprocity between the researcher and her interlocutors and is essential to the study of religion. However, the nature of religious studies as a discipline contributes to a fundamental misunderstanding about the place and importance of feminist ethnography. The normative white, patriarchal, Protestant narratives that form the basis of the field work against its widespread acceptance within the academy. This article brings together scholarship in religious studies with that of anthropology, sociology, and other disciplines to illustrate the significance of feminist ethnography in the study of religion in order to challenge these unspoken and problematic assumptions. By appreciating the outlined “building blocks,” scholars of religion can come to value and potentially employ feminist ethnography in their own work.
Going with the flow: Experimental simulation of sediment transport from a foraminifera perspective
Authors: Ash‐Mor Anat , Almogi‐Labin Ahuva , Bouchet Vincent M. P. , Seuront Laurent , Guy‐Haim Tamar , Ben‐Avraham Zvi , Bookman Revital 
Published: September 2021

AbstractTransport of continental shelf sediments to the deep ocean can be studied from displaced symbiont‐bearing larger benthic foraminifera found in turbidity current deposits. The larger benthic foraminifera habitat depth, physical characteristics and preservation serve as indicators for understanding sediment transport dynamics near the seabed and in the water column. Here, an experiment was designed to explore sediment transport in a closed flume system using simulated high current velocities. Shelf sediments from the Gulf of Eilat/Aqaba, dominated by Amphistegina papillosa and Operculina ammonoides, were subjected to 60 cm s−1 and 80 cm s−1 current velocities while collected in a 10 cm vertical sediment trap. Larger benthic foraminifera abundance, shell physical properties and preservation were analyzed and compared with the original bulk sediments. The experiment results showed that at 80 cm s−1 velocity, larger benthic foraminifera shells of all sizes and preservations are efficiently resuspended and transported in large quantities throughout the water column, as opposed to their transport as bedload by the lower velocity current. Larger benthic foraminifera shape also has a role in the transport distances and accumulation depths. Operculina ammonoides shells were found to be more portable, compared to Amphistegina papillosa, due to their flatter discoid shape. The results suggest that a threshold velocity of ca 80 cm s−1 was needed to generate the thick coarse deposits found in the Gulf of Eilat/Aqaba slope sedimentary record, which were previously suggested to be triggered by large magnitude seismic events. Lower velocities probably winnowed minor amounts of larger benthic foraminifera shells (with little or no coarser sediments) that were deposited as a thin sand layer may point to lower magnitude seismic triggers. In conclusion, larger benthic foraminifera shells are transported and deposited in accordance with their hydrodynamic properties, resulting in assemblage differentiation along the transport pathway. This study shows that the fossil biogenic composition in slope sediments includes valuable information on current velocities, transport dynamics and possible triggers in the geological record.
Perforin, COVID‐19 and a possible pathogenic auto‐inflammatory feedback loop
Authors: Cunningham Louise , Kimber Ian , Basketter David , Simmonds Peter , McSweeney Sheila , Tziotzios Christos , McFadden John P. 
Published: September 2021

AbstractDuring COVID‐19 infection, reduced function of natural killer (NK) cells can lead to both compromised viral clearance and dysregulation of the immune response. Such dysregulation leads to overproduction of cytokines, a raised neutrophil/lymphocyte ratio and monocytosis. This in turn increases IL‐6 expression, which promotes scar and thrombus formation. Excess IL‐6 also leads to a further reduction in NK function through downregulation of perforin expression, therefore forming a pathogenic auto‐inflammatory feedback loop. The perforin/granzyme system of cytotoxicity is the main mechanism through which NK cells and cytotoxic T lymphocytes eliminate virally infected host cells, as well as being central to their role in regulating immune responses to microbial infection. Here, we present epidemiological evidence suggesting an association between perforin expression and resistance to COVID‐19. In addition, we outline the manner in which a pathogenic auto‐inflammatory feedback loop could operate and the relationship of this loop to genes associated with severe COVID‐19. Such an auto‐inflammatory loop may be amenable to synergistic multimodal therapy.
Evaluation of immunodiagnostic tests for human gnathostomiasis using different antigen preparations of Gnathostoma spinigerum larvae against IgE, IgM, IgG, IgG1‐4 and IgG1 patterns of post‐treated patients
Authors: Ieamsuwan Issariya , Watthanakulpanich Dorn , Chaisri Urai , Adisakwattana Poom , Dekumyoy Paron 
Published: September 2021

AbstractObjectivesThe aims of the study were two‐fold: (1) antigen (Ag) preparation and evaluation of three antigens of Gnathostoma spinigerum infective larvae (GsL3), crude somatic antigen (CSAg), excretory‐secretory antigen (ESAg) and partially purified antigens (namely P1Ag, P2Ag and P3Ag) to differentiate IgE, IgG, IgG1–4 and IgM for human gnathostomiasis diagnosis; and (2) application of the selected ELISA for following up stored sera of patients treated with ivermectin (IVM) and albendazole (ABZ).MethodsDifferent antigens were analysed by antibodies of gnathostomiasis cases, other parasite infections and healthy controls using indirect ELISA to differentiate IgE, IgG, IgG1–4 and IgM. Then, prominent antigen and immunoglobulin were used in antibody predictions of gnathostomiasis cases treated with albendazole or ivermectin.ResultsSensitivity of all evaluated ELISAs: IgM‐, IgG‐, IgG1‐ and IgG4‐ELISA, was 100%. IgM‐ELISA with CSAg and P3Ag exhibited the highest specificity of 99%. IgG‐ELISA with P2Ag resulted in the highest specificity of 92.3%. IgG1‐ELISA with P2Ag and P3Ag showed excellent results with 100% specificity. Finally, P2Ag evaluated IgG1 of the followed‐up cases with ABZ and IVM. Decreasing antibody IgG1 levels were mostly found in both treatments at Month 9 and long follow‐up was over 12 months. A Gnathostoma worm was extracted from each two treated patients.ConclusionsUsing IgG1‐ELISA against P2Ag and P3Ag gave excellent results with 100% sensitivity and specificity. These tests can be an alternative to immunoblotting for gnathostomiasis. IgG1 decreased at least 9 months in most cases, so long‐term treatment should be performed over 1 year.