bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Ten-LncRNA Signatures Predict Disease-Free and Overall Survival for Hepatocellular Carcinoma
Authors: Lu Jiong , Yang Sishu , xiong xianze 
Published: June 2021

Abstract
Background: The prognosis of hepatocellular carcinoma (HCC) is bleak though it has been improved over recent years. Early diagnosis could improve the survival. Plenty of researches indicate that long non-coding RNAs (lncRNAs) could play an important role in prognostic prediction of cancer as a kind of biomarker. Methods: We downloaded clinicopathological characteristics and lncRNA expression data of HCC patients from The Cancer Genome Atlas (TCGA) database. The ratio of training sets to validation sets was 2:1. Significant differentially expressed lncRNAs were identified by log-rank test and cox regression. All the significant lncRNAs were selected into the least absolute shrinkage and selection operator regression (LASSO) analysis and constructed risk-score formula by linear combination. Performance of the signatures were validated by receiver operating characteristics (ROC) curves and Kaplan-Meier survival curves. The correlated messenger RNAs (mRNA) were evaluated by functional enrichment analysis. Results: We identified and validated ten-lncRNAs based signatures to predict disease-free survival (DFS) and overall survival (OS) of HCC respectively. Stratified survival analysis showed that the performance of lncRNAs related signatures was better than tumor, node, metastasis(TNM) staging system. Functional enrichment analysis showed that organelle fission and regulation of mRNA metabolic process were significantly enriched in differentially expressed lncRNAs (DElncRNAs). Transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway were significantly enriched pathways in the pathway enrichment analysis. Conclusion: we constructed two lncRNAs based signatures which could predict prognosis of HCC more accurate than the traditional ways.
Phreatic Surface Estimation In MSE Wall With Geocomposite Back Drainage
Authors: Chinkulkijniwat Avirut , Horpibulsuk Suksun , Duong Hai La , Quang Thien Do 
Published: June 2021

Abstract
This study proposes a simple mathematic model for approximating the level of phreatic surface inside the protected zone in mechanical stabilized earth wall with back drain installation though the position of phreatic surface at the drainage interface (ho) which reflects the maximum level of phreatic surface in the protected zone. The proposed model was established based on dataset taken from 180 simulation cases caried out in Plaxis environment. Regression results present a combination of significant effects and major role to maximum water level in the protected zone (ho) of a ratio of length from upstream water to the drainage face to the wall height (L/H), a soil permeabilities coefficient (k) and a transmissivity of the drainage material (Tnet). The proposed model can facilitate design of drainage material to achieve desired level of phreatic surface in the protected zone.
WITHDRAWN: Decellularized Lung Extracellular Matrix Scaffold Promotes Human Embryonic Stem Cell Differentiation Towards Alveolar Progenitors
Authors: Noori Afshin , Dezfouli Mohammad Reza Mokhber , Rajabi Sarah , Ganji Fatemeh , Ghezelayagh Zahra , Agha Elie El , Baharvand Hossein , Chaleshtori Sirous Sadeghian , Tahamtani Yaser 
Published: June 2021

Abstract
Efficient production of alveolar epithelial cells and achieving optimal functionality and maturity has presented a serious challenge in recent years. The extracellular matrix (ECM) provides a dynamic environment and mediates cellular responses during both development and tissue repair. Decellularized ECM (dECM) which retains its native-like structure and biochemical composition provides the signals needed to induce differentiation into tissue-specific lineages in vitro. Here, we repopulated lung ECM-derived scaffold (scaffold) with human embryonic stem cells (hESCs) derived lung progenitor cells and compared with the cells grown on either lung ECM-derived hydrogel (hydrogel) or fibronectin-coated plates in 2D cultures. We found that scaffold preserved its composition and native structures. All groups displayed progenitor cell differentiation as revealed by the expression of NKX2.1, P63, and CK5. Although no significant differences in cell viability between groups, cells differentiated on scaffold and hydrogel showed significant upregulation of SOX9 (a marker of the distal airway epithelium), cells differentiated on scaffold showed enhanced expression of SFTPC (AT2 marker), FOXJ1 (ciliated cell marker) and MUC5A (secretory cell marker). Overall, our results suggest that scaffold improves differentiation of progenitors into alveolar type 2 cells in comparison with hydrogel and fibronectin as well as efficient retention and homing to the alveolar region.
Lactobacillus Plantarum LRCC5314 Includes a Gene for a Substance that Stimulates the Secretion of Serotonin
Authors: Jeong Jiseon , Yoon Seokmin , Kim Jong-Hwa , Kim Wonyong 
Published: June 2021

Abstract
Background: As the functions of probiotics within the same species may not be shared, it is important to analyze the genetic characteristics of strains to determine their safety and usefulness before industrial applications. Results: The complete genome of Lactobacillus plantarum LRCC5314 isolated from kimchi was acquired through PacBio sequencing to confirm probiotic functions and genetic characteristics. Phylogenetic and comparative genomic analyses were performed between L. plantarum LRCC5314 and L. plantarum ATCC 14917T, and the results showed that two strains were highly similar. However, there were some genetic differences. The characteristics of carbon source metabolism were also determined based on the KEGG database and physiological properties. L. plantarum LRCC5314 could metabolize hexoses through homofermentation, which produces only lactic acid. According to gene annotation, L. plantarum LRCC5314 could encode almost complete biosynthetic pathway to produce tryptophan, which can be used as a precursor of serotonin. The serotonin ELISA test using Caco-2 and HT-22 cells treated with a supernatant of the bacterial culture showed that more serotonin was produced by treated cells than by untreated cells.Conclusion: L. plantarum LRCC5314 could provide a source for serotonin production, and L. plantarum LRCC5314 could be used as a functional probiotic for stress regulation.
Discovery of a “Cocktail” of Potential SARS-COV-2 Main Protease Inhibitors through Virtual Screening of Known Chemical Components of Vitex negundo L. (“Lagundi”)
Authors: Cayona Ruel , Creencia Evelyn 
Published: June 2021

Aim:
The prevailing crisis caused by the COVID-19 pandemic demands the development of effective therapeutic agents that can be implemented with minimal to zero adverse effects.

Background:
Vitex negundo L. (VNL) is a medicinal plant with reported efficacy against respiratory diseases and some of the COVID-19 symptoms. Funded by the Department of Science and Technology (DOST), the University of the Philippines – Philippine General Hospital (UP-PGH) is currently conducting clinical trials of VNL and other medicinal plants as adjuvant therapeutic agents against mild cases of COVID-19. The basis for the clinical trials is primarily the pharmacological efficacy of the medicinal plants against respiratory disorders and associated COVID-19 symptoms.

Objective:
This study assessed the in silico potential of VNL components against SARS-CoV-2 main protease (Mpro), an enzyme that plays an important role in COVID-19, the disease caused by the SARS-CoV-2.

Objective:
This study assessed the in silico potential of VNL components against SARS-CoV-2 main protease (Mpro), an enzyme that plays an important role in COVID-19, the disease caused by the SARS-CoV-2.

Method:
Phytochemical mining of VNL components from the literature was conducted. A database consisting of 250 known compounds from different parts of VNL was created and screened against SARS-CoV-2 Mpro using the PyRx virtual screening tool. The most promising components were further subjected to in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) analyses using the SwissADME web server and Toxtree software.

Results:
Virtual screening revealed that 102 VNL components in the database had comparable to or better binding affinities toward SARS-COV-2 Mpro than known chemical inhibitors (e.g. N3 and carmofur). It was determined that the active site of SARS-CoV-2 Mpro receptor consists of multiple H-donor and acceptor sites; hence, the most stable receptor-ligand complexes are generally formed by VNL ligands that establish effective H-bonding with the SARS-CoV-2 Mpro. The promising components, representing a “cocktail” of potential inhibitors also revealed interesting ADMET properties.

Conclusion:
This in silico study identified VNL as a potential single source of a cocktail of SARS-CoV-2 Mpro inhibitors and a promising adjuvant therapeutic agent against COVID-19 or its symptoms. Furthermore, the study offers a rationale on phytochemical mining from medicinal plants as a means that can be implemented in the early stage of a drug discovery and development program.
Optimization and In vitro Evaluation of Famotidine Loaded Effervescent Orally Disintegrating Tablets Using Central Composite Design
Authors: Kumar Ramesh , Verma Ravinder , Kaushik Ritu , Kaushik Prerna , Pandey Parijat , Purohit Deepika , Mittal Vineet , Kaushik Deepak 
Published: June 2021

Background:
Over the years, effervescent orally disintegrating tablets (ODTs) have proved
their worth over conventional tablets in overcoming the swallowing problems associated with the geriatric
and pediatric population. The addition of effervescent agents in ODT provides a rapid disintegration
along with masking of the slight bitter taste of drugs and is worth exploring.

Objective:
The present research investigation deals with the preparation of effervescent ODTs by direct
compression with rapid disintegration and adequate hardness using the central composite design
response surface methodology.

Methods:
Central composite design was used to study the effect of concentration of crospovidone (X1)
and concentration of citric acid and sodium bicarbonate (X2) as independent factors on the two responses:
disintegration time (Y1) and drug release (Y2). The tablets were prepared by direct compression
approach using directly compressible mannitol.

Results:
The optimization results obtained after application of ANOVA revealed that increase in the
concentration of X1 and X2 resulted in the increase in disintegration time and % CDR. Following the
statistical analysis of the results, the formulation batch (F-7) with a desirability index of 0.95 was chosen
as optimized batch and exhibited 99±0.8 % release of the drug after 5 min, and rapid disintegrating
time of 31±3 sec. All other tablet attributes were well within the official limits.

Conclusion:
The results obtained in the present investigation clearly revealed a successful development
of famotidine effervescent ODTs with a better release profile in comparison to marketed formulation.
Wpływ infrastruktury na zachowania kierowców na przejściach dla pieszych na przykładzie Warszawy i Radomia
Authors: Sulmicki Maciej 
Published: June 2021

W 2019 r. przeprowadzone zostały badania wpływu rozwiązań infrastrukturalnych na przejściach dla pieszych i przejazdach dla rowerzystów na zachowania kierowców. Celem ramowym przeprowadzonych badań była weryfikacja trafności zapisów zawartych w dokumentach strategicznych i planistycznych województwa mazowieckiego odnoszących się do bezpieczeństwa ruchu drogowego. Lokalizacje w Warszawie i Radomiu zostały dobrane w taki sposób, aby uwzględnione zostały wszystkie wymienione w Planie zagospodarowania przestrzennego województwa mazowieckiego rodzaje uspokojenia ruchu mające służyć poprawie bezpieczeństwa i dotyczące bezpośrednio przejść dla pieszych. Jednocześnie pod uwagę brane były takie czynniki jak przekrój jezdni, układ skrzyżowania i sygnalizacja świetlna. Podczas badań obserwowano zachowania kierowców względem niezmotoryzowanych oraz te, które determinowały możliwość szybkiego zareagowania na pojawienie się niezmotoryzowanego uczestnika ruchu. Badania były prowadzone z punktów obserwacyjnych oddalonych od skrzyżowania, by nie wpływać na zachowanie uczestników ruchu. Na podstawie co najmniej trzydziestominutowych obserwacji w terenie oraz późniejszej analizy nagrań notowano, czy: kierowca zatrzymuje się przed przejściem, wjeżdża przed lub za niezmotoryzowanego, zatrzymuje się na przejściu, wjeżdża szybko na przejście. W wybranych lokalizacjach analizowano również, czy kierowca rozgląda się przed wjazdem na przejście, jednak w większości miejsc nie było to możliwe ze względu na duże natężenie ruchu i/lub nieodpowiednią widoczność. Obserwacje w Radomiu zostały przeprowadzone przez Sebastiana Pawłowskiego i Łukasza Zaborowskiego z Mazowieckiego Biura Planowania Regionalnego, Oddziału Terenowego w Radomiu. Wyniki badań wskazują, że na niebezpieczne zachowania kierowców mają wpływ: przekrój jezdni na przejściu dla pieszych, obecność przejazdów dla rowerzystów i zasady pierwszeństwa, fizyczne środki uspokojenia ruchu oraz sygnalizacja świetlna. Wykazano, że nieskutecznym rozwiązaniem jest powierzchnia wyłączona z ruchu jedynie za pomocą oznakowania poziomego oraz czerwone światło z dopuszczonym warunkowym prawoskrętem (zieloną strzałką), które traktowane jest jako zielone światło. Badania terenowe potwierdziły słuszność zapisów zawartych w dokumentach strategicznych i planistycznych województwa mazowieckiego, jak też konieczność ich szerszego stosowania. Analiza wyników pozwoliła wykazać konkretne zależności pomiędzy różnymi parametrami rozwiązań a ich wpływem na zachowania kierowców, co stanowi uzupełnienie prowadzonych wcześniej badań lokalnych oraz na poziomie krajowym, przeprowadzonych w 2018 r.
Kluby jeździeckie – oferta elitarna czy dostępna dla wszystkich?
Authors: Pawlikowska-Piechotka Anna , Łukasik Natalia 
Published: June 2021

Tematem artykułu jest rozważenie uwarunkowań rozwoju jeździectwa w aglomeracji warszawskiej. Omówiono w nim uwarunkowania rozwoju, potencjały i najistotniejsze bariery, a także możliwości znoszenia ograniczeń oraz działania na rzecz upowszechnienia sportu i rekreacji jeździeckiej. Jest to jeden z wątków tematycznych badań przeprowadzonych w wybranych ośrodkach jeździeckich w aglomeracji warszawskiej, w ramach dwóch kolejnych statutowych projektów badawczych, finansowanych grantem Ministerstwa Nauki i Szkolnictwa Wyższego ds-114 oraz ds-144. Na podstawie zgromadzonego materiału została przygotowana i obroniona na Akademii Wychowania Fizycznego Józefa Piłsudskiego w Warszawie rozprawa doktorska dr Natalii Łukasik Społeczne i kulturowe uwarunkowania rozwoju jeździectwa w Warszawie i gminach sąsiednich (promotor: prof. dr hab. inż. arch. Anna Pawlikowska-Piechotka). Niniejszy artykuł prezentuje zarys założeń badań, uzyskane rezultaty na podstawie analizy uporządkowanego materiału badań oraz wysunięte syntetyczne wnioski końcowe.
Сomparative epidemiological and demographic characteristics of TB and HIV infections among military personnel of the Russian Federation and USA armed forces and armed forces of National liberation army of people’s Republic of Сhina
Authors: Rusev I. T. , Kuzin A. A. , Malikova E. A. , Kozlov K. V. , Zhdanov K. V. , Beznosik R. V. , Karpushchenko V. G. , Sechin A. A. , Bulygin M. A. , Shinkareva O. G. , Orlova E. S. , Nechaeva Yu. V. 
Published: June 2021

More than 1,7 million people, 770 thousand people from HIV infection, died from tuberculosis worldwide in 2018. In accordance with the United Nations Sustainable Development Goals and the World Health Organization’s strategy to fight tuberculosis, all countries need to reduce the incidence of tuberculosis by 80% and reduce the death rate from tuberculosis by 90% by 2030. According to the Joint United Nations programme on HIV/AIDS (UNAIDS) goals of «90–90–90», countries should achieve the following indicators by 2020: «90% of people living with HIV infection should know their HIV status; 90% of those who know about their HIV infection should receive treatment, and 90% of those who are being treated should not have their viral load determined». Socially significant diseases are a serious problem for the Russian Federation (RF). The RF is one of 30 countries with a high incidence of tuberculosis and ranks 3rd place in multidrug-resistant tuberculosis and 6th place in the prevalence of HIV infection. Socially significant diseases present a serious problem for the Armed Forces of the RF. 150 new cases of tuberculosis, over 50 cases of HIV infection are diagnosed every year among military personnel of the RF Armed Forces.The purpose of the studyis to analyze the current epidemiological situation of tuberculosis and HIV infection in the armed forces of the Russian Federation and foreign armies, to generalize domestic and foreign experience in the organization of medical care for military personnel with these diseases.Data from reports of the medical service and a review of domestic Russian and foreign medical literature allowed us to assess TB and HIV sickness rate among military personnel in the RF and foreign states, as well as to characterize the current system of medical care for this category of patients.
Сlinical and morphological case of cervical cancer due to HIV-associated infection
Authors: Vorobeva O. V. , Lastochkin A. V. 
Published: June 2021

Cervical cancer ranks second in the structure of mortality of malignant tumors of the female reproductive system. Of particular interest is cervical cancer in patients with HIV infection, which develops more rapidly than in HIV-negative women. In this regard, the purpose of our study was to analyze the clinical and morphological case of the development of cervical cancer with HIV infection and to describe the features of metastasis in a young patient.Materials and methods. A postmortem examination was carried out, the material of the accompanying documentation (case history, outpatient card) was studied, a histological examination of the material was carried out (staining with hematoxylin and eosin, immunohistochemical examination for CK5/14, p16, p63).Research results. In a young patient (41 years old), HIV-infected, the cervix is revealed with destructive changes, ulceration, with signs of infiltrative growth, with the growth of the tumor structure into the surrounding tissues. Squamous cell non-keratinizing cancer of the cervix of a moderate degree of differentiation was histologically exposed. In the analyzes — CD4+ lymphocytes 18 cells/ml. The peculiarity and rarity of the presented clinical and morphological case is that against the background of the aggressive course of cervical cancer, there is an absence of characteristic metastases, there is a pronounced infiltrating growth and germination of atypical cells into the surrounding tissues, with the development of severe complications.
Evaluation of the Hydrochemical Characteristics of Groundwater and its Suitability for Desalination to Product Potable Water in Tolul Al-Baj, Salahaldin, Iraq
Authors: Amen Ehab Mohammad , A. Ektifa Taha , A. Mayada Ahmed 
Published: June 2021

The study area, Tlul Al-Baj, suffers from a shortage of fresh water and most people depend on groundwater for different uses (drinking, domestic, irrigation, etc.).
The present research aims to select the most suitable wells for desalination and production of potable water in Tlul Al-Baj area.
Twenty-two samples of groundwater were collected to evaluate the hydrochemical properties of groundwater in the shallow aquifer in the area and to determine their suitability for desalination purposes. The study included measuring the physicochemical characteristics of groundwater, such as total hardness (TH), total dissolved solids(TDS), sodium adsorption ratio (SAR), sodium ratio (Na%), turbidity (Tur), pHâ€¦etc. Chemical analyses for the main components of water samples were also conducted, including cations such as sodium (Na+), potassium (K+), calcium (Ca++), and magnesium (Mg++), as well as anions such as chloride (Cl)-, sulfate (SO4=), bicarbonates (HCO3-), and nitrate (NO3-). In addition, concentrations of trace elements such as iron (Fe), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni),cobalt (CO), chromium(Cr) and cadmium (Cd) were determined.
The results of the physical and chemical analyses for the groundwater of the study area were compared with the international and local standards to determine their suitability for drinking uses and to select the most suitable wells for the production of drinking water by desalination. The results indicated that the most suitable wells for desalination were wells numbered 4, 8, 9, 17, and 19.
Histological Changes in Kidney and Spleen of Albino Mice Treated with Brake Pad Particles
Authors: Al-Easawi Nada Abdulrahman F. , Ghaidan Hajer Qais , Salman Shahad Mansour 
Published: June 2021

In this study, the consequences of treatment with brake pad particles on kidney and spleen were evaluatedthrough microscopic anatomy sections for 60 male albino mice. The animals were divided into six groups, the first three groups (A,B,C) were exposed to brake pad particles depending on periods of exposure (4, 8, and 12weeks, respectively), while the other three groups were control groups,designated asF, which were exposed to laboratory fresh air only. A special locally-designed inhalation chamber was used to expose the animals. The exposure dose to brake pad particles (total suspended particles) was 2.228 Âµg/mÂ³ for 30 min/day, 5 days/week,4, 8 and 12 weeks.The statistical analysis showed that the weights of organs for both kidney and spleen of treated mice had highly significant differences (P< 0.01) compared with control groups.The histological sections examination of the kidney, when compared with the control group, showed subcapsular tubular vascular degeneration and mild cortical focal hemorrhage in group A that was exposedto brake pads particlesfor 4 weeks. In group B, the exposure for 8 weeks resulted in cortical vascular degeneration, cortico-medullary vascular congestion, focal interstitial nephritis, and thickening of interstitial tissue.Other effects included marked dilation of collecting ducts with tubular vascular degeneration, necrosis, glomerular degeneration and deterioration, and cast formation. Group C, exposed for 12 weeks , showed similar changes of the kidney to those of group B. As related to spleen sections,they demonstrated mild subcapsular vascular degeneration of lymphocytes in group A,while the exposure in group Bresulted in moderate amyloid deposits with hemosiderosis, along withmild subcapsular vascular degeneration of lymphocyteswithin the red pulp. In group C, which was treated for 12 months, severe splenomegaly with advanced secondary splenic amyloidosis was also observed within the red pulp.It was characterized by marked deposited acellular and amorphous pinkish homogenous material within red pulp.The continuous exposure to brake pad particles causes harmful effectson the tissues ofessential body organs in human health.These particles should be thought-about as a sort of additional air pollutants in several cities of Iraqin the future.