bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shen-Yuan-Dan Capsule Attenuates Verapamil-Induced Zebrafish Heart Failure and Exerts Antiapoptotic and Anti-Inflammatory Effects via Reactive Oxygen Species–Induced NF-κB Pathway
Authors: Li Sinai , Liu Hongxu , Li Yue , Qin Xiaomei , Li Mengjie , Shang Juju , Xing Wenlong , Gong Yanbing , Liu Weihong , Zhou Mingxue 
Published: March 2021

Background:Heart failure (HF) is the end stage of ischemic cardiovascular diseases; nonetheless, safe and effective therapeutic agents for HF are still lacking, and their discovery remains challenging. Our previous studies demonstrated that Shen-Yuan-Dan Capsule (SYDC), a hospital preparation of traditional Chinese herbal, effectively protected ischemic injury in cardiovascular diseases. However, its therapeutic effects and possible mechanisms on HF remain unclear.Methods: A zebrafish HF model treated with verapamil was developed to assess the therapeutic effect of SYDC on HF zebrafish. Zebrafish were administered with SYDC and digoxin (positive control) by direct soaking. After drug treatment, zebrafish were randomly assigned to the visual observation and image acquisition using a Zebralab Blood Flow System. The reactive oxygen species (ROS), MDA, and SOD levels were determined by fluorescence signal detection, TBA, and WST-8 methods. RT-PCR determined the mRNA expressions of Caspase-3, Caspase-1, Bcl-2, Bax, IL-1β, NF-κB, and TNF-α.Results:SYDC significantly inhibited the levels of heart dilatation and venous congestion and markedly increased the levels of cardiac output, blood flow dynamics, and heart rates in HF zebrafish (p&lt; 0.05,p&lt; 0.01, andp&lt; 0.001). Moreover, SYDC also significantly decreased the levels of MDA and ROS and increased the level of SOD in HF zebrafish. The RT-PCR results revealed that SYDC decreased the expression of Caspase-1, Caspase-3, Bax, IL-1β, NF-κB, and TNF-α but increased the expression of Bcl-2 in HF zebrafish (p&lt; 0.05,p&lt; 0.01, andp&lt; 0.001).Conclusions:SYDC improved the heart function in verapamil-induced HF zebrafish and alleviated inflammation and apoptosis by inhibiting the ROS-mediated NF-κB pathway.
Inhibition of Fibroblast Activation in Uterine Leiomyoma by Components of Rhizoma Curcumae and Rhizoma Sparganii
Authors: Feng Yewen , Zhao Yumin , Li Yao , Peng Teng , Kuang Yu , Shi Xingming , Wang Gang , Peng Fu , Yu Chenghao 
Published: March 2021

Background: The herbs Rhizoma Curcumae and Rhizoma Sparganii (RCRS) are often used in traditional Chinese medicine for the treatment of uterine leiomyoma (UL). The effectiveness of RCRS for the treatment of UL has been confirmed in our previous studies.Purpose: This study aimed to investigate the molecular mechanism by which RCRS inhibits the activation of fibroblast activation protein (FAP) and prevents UL in rats.Study Design and Methods: A Sprague Dawley (SD) rat model of UL was established via estrogen and progesterone load combined with external stimulation. Histological analyses, enzyme-linked immunosorbent assays, and western blotting were performed to evaluate the effect of RCRS on UL and elucidate its mechanism of action.Results: Our data showed that the treatment of SD rats with RCRS significantly reduced the expression of extracellular matrix component collagen, FAP, and transforming growth factor beta (a FAP-activating factor) and the phosphorylation of the cell proliferation pathway-related signaling factors AKT/MEK/ERK.Conclusion: Our results suggest that RCRS is effective in the prevention and treatment of UL in rats, and RCRS may exert its functions by inhibiting the activation of tumor-associated fibroblasts and cell proliferation and by improving the tumor extracellular matrix.
Excavating the Role of Relationship in Deshpande’s through the Lens of Psychoanalysis
Authors: Shelonitta P 
Published: March 2021

The main objective of this paper entitled Shashi Deshpande’s Strangers to Ourselves: A Psychoanalytical Perspective analyzes the impact of intricate web of family and the complexities of relationships in the making of a happy family. The work also tries to capture the Man-Women Relationship and further discusses the lack of understanding in their conjugal life.
Pensar la autonomía puigiana en tiempo de un orden mundial en transición
Authors: Simonoff Alejandro 
Published: March 2021

Juan Carlos Puig pensó la factibilidad de márgenes de maniobra en un entorno internacional relativamente estable.
Sin embargo, desde el fin de la guerra fría, venimos observando la falta de conformación de un orden mundial y cómo éste afecta a todos los países, y en particular para el caso de Argentina.
Por ello a partir de esa situación y teniendo en cuentan los lineamientos que este pensador del Cono Sur dio al concepto de autonomía, nos proponemos hacer una lectura de ellos, para establecer algunos parámetros de certitud en nuestro análisis.
Abordagem histórica da assistência de enfermagem em tempos de pandemias durante o século XIX ao XXI
Authors: Pinheiro Evangelista Brenda , Pinheiro Evangelista Breno , Camilo Pereira Michelly , Ferreira Silva Luiza Maria , Germano de Lima Solange Maria , De Freitas Kerma Márcia 
Published: March 2021

Objetivo: analisar, por meio da literatura, as contribuições da assistência de enfermagem no contexto das pandemias. Método: revisão integrativa da literatura realizada na Biblioteca Virtual em Saúde (BVS) e Scientific Eletronic Library Online (SciELO) e PubMed com os Descritores em Ciências da Saúde (DeCS) "assistência integral í saúde"; "cuidados de Enfermagem" e "pandemias". Os critérios de inclusão foram: texto em português e inglês e de exclusão foram desconsiderados artigos em formatos de textos, que não passaram por processos rigorosos de avaliação. Resultados: A primeira categoria mostra os profissionais da enfermagem superam desafios em saúde por intermédio da assistência qualificada, o cuidado holí­stico e o plano de cuidados desses profissionais refletiram na recuperação. A segunda categoria enfatiza que as teorias de enfermagem implementadas no contexto histórico da enfermagem moderna no cenário atual. Conclusão: As contribuição da assistência de enfermagem quanto as pandemias vivenciadas, coloca a enfermagem como protagonista.
Influência da equoterapia na qualidade de vida de idosos caracterizados com a classificação internacional de funcionalidade – CIF
Authors: Dubiela Julik Angela , Miri Andressa Leticia , Dorigoni Bini Ana Carolina , Pacheco Tyski Suckow Patricia , Gonçalves de Jesus Fonseca Eliane , Figueiredo Moreira Felipe , Carraro Emerson 
Published: March 2021

Objetivo: Caracterizar idosos ativos através de Core Set da Classificação Internacional de Funcionalidade (CIF) para saúde de idosos e avaliar a qualidade de vida por meio do The 36-Item Short Form Health Survey- SF-36 na terapia assistida com equinos. Métodos: Trata-se de um estudo observacional analí­tico transversal, onde 10 idosos ativos entre 60 í 80 anos realizaram 16 sessões de terapia assistida com equinos, com duração de 30 minutos cada. Para avaliação foi utilizado o questionário da CIF, o SF-36 e o Mini Exame do Estado Mental – MEEM. Foi realizado análise estatí­stica descritiva e também foi utilizado o teste t pareado. Resultados: O MEEM pré intervenção não demonstrou declí­nio cognitivo. Na CIF, os domí­nios I, II e III ficaram com qualificadores predominantemente em 0 e 2, já o domí­nio IV as pontuações ficaram com qualificadores entre 3 e 4. Conclusão: A terapia assistida com equinos em idosos é uma alternativa terapêutica efetiva na melhora da qualidade de vida.
THE EFFECT OF USING REAP (READ, ENCODE, ANNOTATE PONDER) METHOD ON STUDENTS’ READING COMPREHENSION IN ANALYTICAL EXPOSITIONTEXT OF THE ELEVENTH GRADEOF SMK SWASTA HARAPAN STABAT IN ACADEMIC YEAR 2019/2020
Authors: Riansyah Deden , Yultisa Nudia 
Published: March 2021

The objective of this research was to find out whether there was a significant effect of using REAP (Read, Encode, Annotate, Ponder) on students’ reading comprehension in analitycal exposition text of the eleventh grade of SMK Swasta Harapan Stabat in academic year 2019/2020. In this research, the researcher took 80 students as the sample. As the experimental class, the researcher used class XI.1 ADP which consisted of 40 students and for the control class, the researcher used class XI.4 ADP that consist of 40 students. The, the researcher gave pre-test and post-test to both classes.The researcher then statistically analyzed the data by using t-test. After analyzing the data, the researcher concluded that teaching analytical exposition text using REAP method was effective. As the fact, the result of t-test was 2,86 with df = 78. Because there was not df 78 in the t-table, so the researcher decided to use df 60 since it is closer to 78 than df 120.The table at df 60 showed that the value of t-table ( was 2,00 for 5% and 2,66 for 1%. Theoritically, the Alternative Hypothesis ( ) was accepted if the value of t-test was greater than the value of t-table ( > ( . The finding showed that t-test was greater than t-table (2,86 > 2,00 and 2,66). The researcher meant the hypothesis ( ) was accepted. It was stated that “There is a significant effect of using REAP method on students’ reading comprehension in analytical exposition text of the eleventh grade students of SMK Swasta Harapan Stabat in academic year 2019/2020” was accepted.
DAMPAK PEMBELAJARAN DARING BAGI MAHASISWA DI MASA PANDEMI COVID-19
Authors: Afni Khairina 
Published: March 2021

Penelitian ini merupakan studi literatur tentang dampak pembelajaran daring bagi mahasiswa di masa pendidikan pandemi Covid-19 yang bertujuan mengidentifikasi dampak dalam melakukan pembelajaran secara daring bagi mahasiswa di masa pandemi Covid-19 ini. Metode yang dipakai dalam penelitian ini adalah metode studi kepustakaan dengan mencari literatur yang terkait dengan fokus apa yang akan dilteliti atau menggunakan data skunder. Hasilnya menunjukkan bahwa selama melakukan pembelajaran daring di masa pandemi Covid-19 yang telah berjalan hampir setahun ini menunjukkan bahwa mahasiswa memiliki masalah atau dampak terhadap poembelajaran daring yang telah mereka lalui. dampak tersebut dapat mengarah pada perubahan belajar yang positif maupun negatif, untuk itu perlu peninjauan kembali terhadap pembelajaran daring yang dipakai baik penggunaan jenis medianya ataupun metode pembelajarannya agar dampak tersebut tidak terlalu monoton diterima siswa.
Urethral Injury Treatment Challenge. Comparison of Surgical Treatments for Acquired or Iatrogenic Urethral Stenosis and Predictive Values for Failure of Each Surgical Method
Authors: Güler Yavuz 
Published: March 2021

Introduction: Minimal invasive endo-urologic interventions and prostate surgeries performed through the urethral route are the most commonly used surgeries in the urology field at present. One of the unwanted complications weeks after some of these surgeries is urethral stenosis.
Aim: In this study, we aimed to review our treatment methods for patients with urethral stricture occurring after urologic interventions retrospectively to evaluate our success rates and data predicting recurrence and to determine the most feasible method.
Materials and methods: From 2008 to 2017, we evaluated retrospectively 234, 85, and 51 patients with urethral stenosis due to iatrogenic causes treated with direct visual internal urethrotomy (DVIU), anastomotic excision urethroplasty, and buccal graft urethro-plasty, respectively. Postoperative Qmax above 15 mL/s and >17 CH cystoscope easily passing the urethra was accepted as success for patients. Group variables were compared using ANOVA. Multivariate logistic regression analysis was performed to determine variables with predictive values. Predictive values significant in regression analysis (p<0.005) were evaluated with ROC analysis.
Results: Patients were followed up for mean 48 (16-56) months, 48 (23-60) months, and 50 (32-65) months after DVIU, anastomotic and graft urethroplasty, respectively, and success was obtained for 75 (32.1%), 71 (83.5%) and 42 patients (82.4%). Multivariate logistic regression analysis found age, DM, BMI and stenosis length were independent predictive factors for DVIU success, while stenosis length was the independent predictive factor for excision anastomosis and buccal graft urethroplasty success. Cut-off values were determined as 56.5 years of age, 30.2 kg/m2 BMI and 1.85 mm stenosis length for DVIU patients, while 2.15 mm and 5.9 mm stenosis lengths, &nbsp;respectively, were determined for excision anastomosis and buccal graft urethroplasty with ROC analysis.
Conclusions: DVIU is a failed method for urethral stenosis in the long term. In contrast, resection / anastomosis and graft urethro-plasty are very successful methods.
The Mechanical Effects of Suture Anchor Insertion Angle for Rotator Cuff Repair
Authors: Liporace Frank A , Bono Christopher M , Caruso Steven A , Weiner Bradley , Penny Keith , Feldman Andrew J , Grossman Mark G , Haher Thomas R 
Published: March 2021

Abstract
Twelve matched pairs of humerii were instrumented with suture anchor at 90°, 75°, 45°, and 30° relative to the cortical border at the junction of the greater tuberosity and articular surface. Two fixtures were inserted into each specimen at different angles and loaded to failure. Suture anchors failed at an average of 171, 219, 169, and 192 N with 90°, 75°, 45°, and 30° insertion angles, respectively. No statistical difference was detected between groups (P=.08). Although previous authors have prescribed angles ^45° to improve pull-out strength, the current in vitro data does not support these recommendations.
Pomen negradbenih ukrepov za poplavno varnost // The importance of non-structural flood protection measures
Authors: Komac Blaž , Zorn Matija 
Published: March 2021

Članek prinaša pregled glavnih tipov, temeljnih lastnosti in učinkov negradbenih protipoplavnih ukrepov. Ugotavljajmo, da uporaba negradbenih ukrepov izhaja iz geografskih raziskav in da so v zadnjih desetletjih vse pomembnejši tudi v drugih strokah, ki se ukvarjajo s poplavami. Izkazalo se je namreč, da samo gradbeni protipoplavni ukrepi ne zadoščajo več za tako kompleksno sodobno družbo, ki jo sicer zaznamujejo tehnizacija, individualizacija in informacijske tehnologije, po drugi strani pa pogrešamo poznavanje nevarnih naravnih procesov, kot so poplave. Negradbeni ukrepi obsegajo širok spekter dejavnosti, ki segajo vse od ukrepov upravljanja porečij in coniranja oziroma vzpostavitve evidenc poplavne ogroženosti, do monitoringa poplav in opozarjanja pred njimi ter ozaveščanja in nenazadnje tudi gospodarskih dejavnosti, kot je zavarovalništvo. Izziv ostaja, kako voditi in povezati negradbene ukrepe, da bi dolgoročno prispevali k poplavni varnosti – kot najbolj učinkovita se je izkazala kombinacija različnih vrst ukrepov, kjer nekateri vplivajo dolgoročno, drugi pa prispevajo kratkoročno.

The article provides an overview of the main types, basic properties and effects of non-structural flood protection measures. The authors note that the use of non-structural measures stems from geographical research and that they have become increasingly important in other flood-dealing disciplines in recent decades. It turned out that structural flood protection measures alone are no longer enough for a complex modern society, which is characterized by technicalization, individualization and information technology, but on the other hand we lack knowledge of natural hazards, such as floods. Non-structural measures cover a wide range of activities, ranging from river basin management and zoning measures or the establishment of flood risk records to flood monitoring and warning, as well as awareness-raising and, last but not least, economic activities such as insurance. The challenge remains how to manage and integrate non-structural measures to contribute to flood safety in the long term – a combination of different types of measures has proven to be most effective, with some having long-term effects and others contribute to short-term effects.
Building Moral Resiliency Through the Nurse Education and Support Team Initiative
Authors: DeGrazia Michele , Porter Courtney , Sheehan Anne , Whitamore Stephanie , White Deborah , Nuttall Penny Winter , Blanchard Tyler , Davis Norah , Steadman Jennifer , Hickey Patricia 
Published: March 2021

Background
Findings from 3 nurse-led research studies conducted in a large pediatric institution resulted in a call to action to support intensive and progressive care nurses experiencing moral and ethical challenges.

Objective
To evaluate the feasibility of and satisfaction with implementation of a Nurse Education and Support Team (NEST) coach role.

Methods
An interdisciplinary work group identified solutions for just-in-time support, including a new NEST coach role. This role was implemented in January 2017 to provide peer-to-peer support for nurses. The NEST coaches provide coverage 5 days per week in 4 intensive care units and 1 progressive care unit. Feasibility of the role was evaluated by assessing the number, type, length, and outcome of NEST coach consultations. Staff satisfaction was evaluated 6 months and 1.5 years after implementation.

Results
A total of 6262 NEST coach consultations occurred across the units from January 2017 through November 2019. At both evaluation periods, more than 85% of respondents indicated that they were satisfied with their interactions with the NEST coach and nearly 80% indicated that they would seek consultation again.

Conclusions
Pediatric intensive and progressive care nurses experience many challenges in their practice environments. The innovative NEST coach role enabled access to just-in-time support and guidance through morally and ethically challenging situations. As evidenced by the number of consultations and the positive staff response, intensive and progressive care nurses have embraced and integrated the NEST coach role into their culture and practice.
Statistical optimization of culture conditions for expression of recombinant humanized anti-EpCAM single-chain antibody using response surface methodology
Authors: Behravan Aidin , Hashemi Atieh 
Published: March 2021

Background and purpose:
The epithelial cell adhesion molecule (EpCAM), is one of the first cancer- associated markers discovered. Its overexpression in cancer stem cells, epithelial tumors, and circulating tumor cells makes this molecule interesting for targeted cancer therapy. So, in recent years scFv fragments have been developed for EpCAM targeting.

Experimental approach:
In this study, an scFv against EpCAM extracellular domain (EpEX) derived from 4D5MOC-B humanized mAb was expressed in Escherichia coli k12 strain, and in order to obtain the optimum culture conditions in chemically defined minimal medium, response surface methodology (RSM) was employed. According to the RSM-CCD method, a total of 30 experiments were designed to investigate the effects of various parameters including isopropyl-b-D-thiogalactopyranoside (IPTG) concentration, cell density before induction, post-induction time, and post-induction temperature on anti EpEX-scFv expression level.

Findings/Results:
At the optimum conditions (induction at cell density 0.8 with 0.8 mM IPTG for 24 h at 37 °C), the recombinant anti EpEX-scFv was produced at a titer of 197.33 μg/mL that was significantly consistent with the prediction of the model.

Conclusion and implication:
The optimized-culture conditions obtained here for efficient production of anti EpEX-scFv in shake flask cultivation on a chemically defined minimal medium could be applied to large- scale fermentation for the anti EpEX-scFv production.
Cytokine Profiles in Periodontitis and COVID-19
Authors: Hajizadeh Farhad , Houshmand Behzad , Ekhlasmandkermani Mehdi , Khazaei Saber , Kheiri Aida 
Published: March 2021

Introduction:
SARS-CoV-2 is a novel coronavirus that causes an infectious disease named COVID-19. Respiratory distress syndrome and multiple organ failure are the common outcomes of COVID-19 that may finally lead to death. During COVID-19, cytokine storm takes place that is known by the release of notable amounts of pro-inflammatory cytokines including IL-1, IL-6, and TNF-α.

The Hypothesis:
Periodontal disease are a group of inflammatory diseases in which elevated levels of some cytokine such as IL-1, IL-2, IL-5, IL-6, IL-10, and TNF-α are observed in patients’ serums. Due to the similarity of cytokine expressions in these two diseases, there might be a possible association between COVID-19 and periodontitis, especially the chronic type. Also, genotype polymorphisms of IL-1, IL-6, IL-10, and TNF-α have been proposed to be in association with chronic periodontitis susceptibility.

Evaluation of the Hypothesis:
assessment of these polymorphisms may also play a significant role in detection of these diseases.
Relaparotomy after Cesarean Section in a Tertiary Center
Authors: Poudel Sandesh , Adhikari Shree Prasad , Rai Snigdha , Mishra Sangeeta , Tamrakar Renuka 
Published: March 2021

Background: Relaparotomy after cesarean section is associated with significant maternal morbidity and mortality which can be prevented with proper precaution during the primary surgery. The objective of the study was to determine factors associated with relaparotomy after cesarean section.
Methods: A hospital based descriptive cross sectional study conducted by analyzing the records of 3 years from 1st July 2017 to 30th June 2020, among the women who underwent relaparotomy after cesarean section at Paropakar Maternity and Women’s Hospital, Kathmandu.
Results: There were 21,270 cesarean sections among 64,475 deliveries with the mean incidence of 32.6%. A total of 22 patients underwent relaparotomy out of which 18 had their primary surgery performed at our hospital i.e. 0.08% of the total caesarean section, and 4 cases were referred from peripheries. 60% of primary surgery were done by residents and the commonest indications of primary surgery were previous CS (36.3%) and obstructed labor (27.2%). Primary PPH was the major indication for laparotomy (31.8%) with mean interval from primary surgery of 4.7 hours followed by burst abdomen (22.7%) and secondary PPH (18.1%). Hysterectomy was the commonest operative intervention done during relaparotomy accounting for 45.4% followed by tension suture for burst abdomen (18%). There were 2 maternal death among them which were due to aspiration pneumonia following eclapmsia and DIC.
Conclusion: Relaparotomy has increased risk of adverse maternal outcome but still is a lifesaving intervention. Early recognition, timely intervention and multidisciplinary teamwork helps to decrease the complications associated with it.
Landscape Below Ground IV: Introduction to a Special Issue
Authors: Watson Gary 
Published: March 2021

AbstractThis special issue is dedicated to research presented at the 2018 Landscape Below Ground IV conference. We describe highlights from the ten original research articles included in this issue. With these articles, we aim to support the advancement of tree care practices and inform managers and policymakers to choose, site, plant, and care for healthier, longer-lived trees by focusing on their critical and vulnerable root systems and the soil that surrounds them.