bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Delayed Ionospheric Response to the 27‐day Solar Rotation Period Analyzed With GOLD and IGS TEC Data
Authors: Schmölter Erik , Berdermann Jens , Codrescu Mihail 
Published: January 2021

AbstractThe delayed ionospheric response is analyzed for two well‐defined 27‐day solar rotation periods in the year 2019 with solar radio flux index F10.7 and Global‐scale Observations of the Limb and Disk (GOLD) data, like solar extreme ultraviolet (EUV) flux proxy, O/N2 column density ratio and peak electron density, as well as International Global Navigation Satellite System Service rapid high‐rate total electron content (TEC) map data. Although the correlation between GOLD solar EUV flux proxy and TEC is similar to the correlation between F10.7 and TEC, it is shown that the estimated delays based on GOLD data are in much better agreement with recent studies using EUV measurements compared to the delays based on F10.7 data. The GOLD peak electron density correlates well with TEC and allows insight to a local time interval when the ionosphere is not controlled by solar activity changes (17:00 LT to 21:00 LT). The present study investigates the impact of the solar activity (F10.7, GOLD EUV flux proxy) and O/N2 column density ratio on the ionospheric delay for two representative solar rotation periods. The capabilities of GOLD data for future research on the ionospheric response to the 27‐day solar rotation period are demonstrated and discussed. These results are crucial information for precise ionospheric models and forecasts.
A Lagrangian Perspective on Tropical Anvil Cloud Lifecycle in Present and Future Climate
Authors: Gasparini Blaž , Rasch Philip J. , Hartmann Dennis L. , Wall Casey J. , Dütsch Marina 
Published: January 2021

AbstractThe evolution of tropical anvil clouds from their origin in deep convective cores to their slow decay determines the climatic effects of clouds in tropical convective regions. Despite the relevance of anvil clouds for climate and responses of clouds to global warming, processes dominating their evolution are not well understood. Currently available observational data reveal instantaneous snapshots of anvil cloud properties, but cannot provide a process‐based perspective on anvil evolution. We therefore conduct simulations with the high resolution version of the exascale earth system model in which we track mesoscale convective systems over the tropical Western Pacific and compute trajectories that follow air parcels detrained from peaks of convective activity. With this approach we gain new insight into the anvil cloud evolution both in present day and future climate.Comparison with geostationary satellite data shows that the model is able to simulate maritime mesoscale convective systems reasonably well. Trajectory results indicate that anvil cloud lifetime is about 15 h with no significant change in a warmer climate. The anvil ice mixing ratio is larger in a warmer climate due to a larger source of ice by detrainment and larger depositional growth leading to a more negative net cloud radiative effect along detrained trajectories. However, the increases in sources are counteracted by increases in sinks of ice, particularly snow formation and sedimentation. Furthermore, we find that the mean anvil cloud feedback along trajectories is positive and consistent with results from more traditional cloud feedback calculation methods.
Regional Exhumation and Tectonic History of the Shanxi Rift and Taihangshan, North China
Authors: Clinkscales Christopher , Kapp Paul , Thomson Stuart , Wang Houqi , Laskowski Andrew , Orme Devon A. , Pullen Alex 
Published: January 2021

AbstractThis study presents a comprehensive low‐temperature thermochronometric data set from the Shanxi Rift, Taihangshan, and eastern Ordos block in North China, including new apatite fission track and apatite (U‐Th‐Sm)/He data and published apatite and zircon fission track and (U‐Th‐Sm)/He data. We use these data and new thermal history inversion models to reveal that the Shanxi Rift and Taihangshan experienced an increase in cooling rates between ca. 110–70 Ma and ca. 50–30 Ma. A preceding ca. 160–135 Ma cooling event is generally restricted to the western rift margin in the Lüliangshan and Hengshan. In contrast, the ca. 50–30 Ma cooling event was widespread and occurred coevally with the opening of the Bohai Basin and slip across the NNE‐striking Eastern Taihangshan fault. In the southern rift zone, however, exhumation beginning ca. 50 Ma was likely associated with fault block uplift across the ESE–striking Qinling and Huashan faults, which accompanied the extensional opening of the Weihe Graben. Coeval fault slip along the NNE–striking Eastern Taihangshan faults and ESE–striking Qinling and Huashan faults was associated with NW‐SE extension in North China related to oblique subduction of the Pacific plate under Eastern Asia and slow convergence rates. The Shanxi Rift is commonly attributed to Late Miocene and younger extension, but our new thermochronologic data do not precisely record the onset of rifting. However, our inversion models do suggest ≤∼50°C of Neogene–Quaternary cooling, consistent with ≤∼2 km of footwall uplift across most range‐bounding faults.
Enhanced third-harmonic generation by manipulating the twist angle of bilayer graphene
Authors: Ha Seongju , Park Nam Hun , Kim Hyeonkyeong , Shin Jiseon , Choi Jungseok , Park Sungmin , Moon Ji-Yun , Chae Kwanbyung , Jung Jeil , Lee Jae-Hyun , Yoo Youngdong , Park Ji-Yong , Ahn Kwang Jun , Yeom Dong-Il 
Published: January 2021

AbstractTwisted bilayer graphene (tBLG) has received substantial attention in various research fields due to its unconventional physical properties originating from Moiré superlattices. The electronic band structure in tBLG modified by interlayer interactions enables the emergence of low-energy van Hove singularities in the density of states, allowing the observation of intriguing features such as increased optical conductivity and photocurrent at visible or near-infrared wavelengths. Here, we show that the third-order optical nonlinearity can be considerably modified depending on the stacking angle in tBLG. The third-harmonic generation (THG) efficiency is found to significantly increase when the energy gap at the van Hove singularity matches the three-photon resonance of incident light. Further study on electrically tuneable optical nonlinearity reveals that the gate-controlled THG enhancement varies with the twist angle in tBLG, resulting in a THG enhanced up to 60 times compared to neutral monolayer graphene. Our results prove that the twist angle opens up a new way to control and increase the optical nonlinearity of tBLG, suggesting rotation-induced tuneable nonlinear optics in stacked two-dimensional material systems.
Interplay between Yersinia pestis and its flea vector in lipoate metabolism
Authors: Bouvenot Typhanie , Dewitte Amélie , Bennaceur Nadia , Pradel Elizabeth , Pierre François , Bontemps-Gallo Sébastien , Sebbane Florent 
Published: January 2021

Abstract
To thrive, vector-borne pathogens must survive in the vector’s gut. How these pathogens successfully exploit this environment in time and space has not been extensively characterized. Using Yersinia pestis (the plague bacillus) and its flea vector, we developed a bioluminescence-based approach and employed it to investigate the mechanisms of pathogenesis at an unprecedented level of detail. Remarkably, lipoylation of metabolic enzymes, via the biosynthesis and salvage of lipoate, increases the Y. pestis transmission rate by fleas. Interestingly, the salvage pathway’s lipoate/octanoate ligase LplA enhances the first step in lipoate biosynthesis during foregut colonization but not during midgut colonization. Lastly, Y. pestis primarily uses lipoate provided by digestive proteolysis (presumably as lipoyl peptides) rather than free lipoate in blood, which is quickly depleted by the vector. Thus, spatial and temporal factors dictate the bacterium’s lipoylation strategies during an infection, and replenishment of lipoate by digestive proteolysis in the vector might constitute an Achilles’ heel that is exploited by pathogens.
Cable bacteria extend the impacts of elevated dissolved oxygen into anoxic sediments
Authors: Liu Feifei , Wang Zhenyu , Wu Bo , Bjerg Jesper T , Hu Wenzhe , Guo Xue , Guo Jun , Nielsen Lars Peter , Qiu Rongliang , Xu Meiying 
Published: January 2021

Abstract
Profound biogeochemical responses of anoxic sediments to the fluctuation of dissolved oxygen (DO) concentration in overlaying water are often observed, despite oxygen having a limited permeability in sediments. This contradiction is indicative of previously unrecognized mechanism that bridges the oxic and anoxic sediment layers. Using sediments from an urban river suffering from long-term polycyclic aromatic hydrocarbons (PAHs) contamination, we analyzed the physicochemical and microbial responses to artificially elevated DO (eDO) in the overlying water over 9 weeks of incubation. Significant changes in key environmental parameters and microbial diversity were detected over the 0–6 cm sediment depth, along with accelerated degradation of PAHs, despite that eDO only increased the porewater DO in the millimeter subfacial layer. The dynamics of physicochemical and microbial properties coincided well with significantly increased presence of centimeter-long sulfide-oxidizing cable bacteria filaments under eDO, and were predominantly driven by cable bacteria metabolic activities. Phylogenetic ecological network analyses further revealed that eDO reinforced cable bacteria associated interspecific interactions with functional microorganisms such as sulfate reducers, PAHs degraders, and electroactive microbes, suggesting enhanced microbial syntrophy taking advantage of cable bacteria metabolism for the regeneration of SO42− and long-distance electron transfer. Together, our results suggest cable bacteria may mediate the impacts of eDO in anaerobic sediments by altering sediment physiochemical properties and by reinforcing community interactions. Our findings highlight the ecological importance of cable bacteria in sediments.
Pilot validation of blood-based biomarkers during pregnancy and postpartum in women with prior or current depression
Authors: Redei E. E. , Ciolino J. D. , Wert S. L. , Yang A. , Kim S. , Clark C. , Zumpf K. B. , Wisner K. L. 
Published: January 2021

AbstractMajor depressive disorder (MDD) is more common in women than in men, and evidence of gender-related subtypes of depression is emerging. Previously identified blood-based transcriptomic biomarkers distinguished male and female subjects with MDD from those without the disorder. In the present pilot study, we investigated the performance of these biomarkers in pregnant and postpartum women with prior major depressive episodes, some of whom had current symptomatology. The symptom scores of 13 pregnant and 15 postpartum women were identified by the Inventory of Depressive Symptoms (IDS-SR-30) at the time of blood sampling. Blood levels of the 20 transcriptomic biomarkers and that of estrogen receptor 2 (ESR2), membrane progesterone receptor alpha and beta (mPRα, mPRβ) were measured. In pregnant women, transcript levels of ADCY3, ASAH1, ATP11C, CDR2, ESR2, FAM46A, mPRβ, NAGA, RAPH1, TLR7, and ZNF291/SCAPER showed significant association with IDS-SR-30 scores, of which ADCY3, FAM46A, RAPH1, and TLR7 were identified in previous studies for their diagnostic potential for major depression. ASAH1 and ATP11C were previously also identified as potential markers of treatment efficacy. In postpartum women, transcript levels of CAT, CD59, and RAPH1 demonstrated a trend of association with IDS-SR-30 scores. Transcript levels of ADCY3, ATP11C, FAM46A, RAPH1, and ZNF291/SCAPER correlated with ESR2 and mPRβ expressions in pregnant women, whereas these associations only existed for mPRβ in postpartum women. These results suggest that a blood biomarker panel can identify depression symptomatology in pregnant women and that expression of these biomarker genes are affected by estrogen and/or progesterone binding differently during pregnancy and postpartum.
Maternal acute and chronic inflammation in pregnancy is associated with common neurodevelopmental disorders: a systematic review
Authors: Han Velda X. , Patel Shrujna , Jones Hannah F. , Nielsen Timothy C. , Mohammad Shekeeb S. , Hofer Markus J. , Gold Wendy , Brilot Fabienne , Lain Samantha J. , Nassar Natasha , Dale Russell C. 
Published: January 2021

AbstractInflammation is increasingly recognized as a cause or consequence of common problems of humanity including obesity, stress, depression, pollution and disease states such as autoimmunity, asthma, and infection. Maternal immune activation (MIA), triggered by both acute and systemic chronic inflammation, is hypothesized to be one of the mechanisms implicated in the pathogenesis of neurodevelopmental disorders (NDD). Although there is substantial preclinical evidence to support the MIA hypothesis, the human evidence is disparate. We performed a systematic review on human studies examining associations between maternal inflammatory states and offspring NDDs (autism spectrum disorder- ASD, attention deficit hyperactivity disorder-ADHD, Tourette syndrome-TS). 32 meta-analyses and 26 additional individual studies were identified. Maternal states associated with ASD include obesity, gestational diabetes mellitus, pre-eclampsia, pollution, stress, depression, autoimmune diseases, and infection. Maternal states associated with ADHD include obesity, pre-eclampsia, smoking, low socioeconomic status (SES), stress, autoimmune disease, and asthma. Maternal states associated with TS include low SES, depression, and autoimmune diseases. Diverse maternal inflammatory states in pregnancy are associated with common offspring NDDs. Given the increased prevalence of NDDs, there is urgent need to explore relative and cumulative maternal risk factors and disease mechanisms. Defining preventable risk factors in high-risk pregnancies could mitigate the expression and severity of NDDs.
EEF1A2 interacts with HSP90AB1 to promote lung adenocarcinoma metastasis via enhancing TGF-β/SMAD signalling
Authors: Jia Liqing , Ge Xiaolu , Du Chao , Chen Linna , Zhou Yanhong , Xiong Wei , Xiang Juanjuan , Li Guiyuan , Xiao Gaoming , Fang Li , Li Zheng 
Published: January 2021

Abstract
Background
Eukaryotic protein translation elongation factor 1α2 (EEF1A2) is an oncogene that promotes the progression of breast and pancreatic cancer. In this study, we aimed to elucidate the oncogenic function of EEF1A2 in the metastasis of lung adenocarcinoma (LUAD).

Methods
Immunohistochemistry and western blot were used to study EEF1A2 expression levels in LUAD tissues and cells, respectively. The role of EEF1A2 in LUAD progression were investigated in vitro and in vivo. We identified potential EEF1A2-binding proteins by liquid chromatography-electrospray mass spectrometry (LC-MS)/MS. Protein–protein interactions were determined by immunofluorescence and co-immunoprecipitation (Co-IP).

Results
In this study, we report that EEF1A2 mediates the epithelial–mesenchymal transformation (EMT), to promote the metastasis of LUAD cells in vitro and in vivo. Moreover, EEF1A2 interacts with HSP90AB1 to increase TGFβ Receptor (TβR)-I, and TβRII expression, followed by enhanced SMAD3 and pSMAD3 expression and nuclear localisation, which promotes the EMT of LUAD cells. Overexpression of EEF1A2 in cancer tissues is associated with poor prognosis and short survival of patients with LUAD.

Conclusions
These findings underscore the molecular functions of EEF1A2 in LUAD metastasis and indicate that EEF1A2 represents a promising target in the treatment of aggressive LUAD.
Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage
Authors: Li Rui , Wang Beini , Wu Chengbiao , Li Duohui , Wu Yanqing , Ye Libing , Ye Luxia , Chen Xiongjian , Li Peifeng , Yuan Yuan , Zhang Hongyu , Xie Ling , Li Xiaokun , Xiao Jian , Wang Jian 
Published: January 2021

AbstractProlonged type 2 diabetes mellitus (T2DM) produces a common complication, peripheral neuropathy, which is accompanied by nerve fiber disorder, axon atrophy, and demyelination. Growing evidence has characterized the beneficial effects of acidic fibroblast growth factor (aFGF) and shown that it relieves hyperglycemia, increases insulin sensitivity, and ameliorates neuropathic impairment. However, there is scarce evidence on the role of aFGF on remodeling of aberrant myelin under hyperglycemia condition. Presently, we observed that the expression of aFGF was rapidly decreased in a db/db T2DM mouse model. Administration of exogenous aFGF was sufficient to block acute demyelination and nerve fiber disorganization. Furthermore, this strong anti-demyelinating effect was most likely dominated by an aFGF-mediated increase of Schwann cell (SC) proliferation and migration as well as suppression of its apoptosis. Mechanistically, the beneficial biological effects of aFGF on SC behavior and abnormal myelin morphology were likely due to the inhibition of hyperglycemia-induced oxidative stress activation, which was most likely activated by kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid-derived-like 2 (Nrf2) signaling. Thus, this evidence indicates that aFGF is a promising protective agent for relieving myelin pathology through countering oxidative stress signaling cascades under diabetic conditions.
Nanohole-boosted electron transport between nanomaterials and bacteria as a concept for nano–bio interactions
Authors: Shi Tonglei , Hou Xuan , Guo Shuqing , Zhang Lei , Wei Changhong , Peng Ting , Hu Xiangang 
Published: January 2021

AbstractBiofilms contribute to bacterial infection and drug resistance and are a serious threat to global human health. Antibacterial nanomaterials have attracted considerable attention, but the inhibition of biofilms remains a major challenge. Herein, we propose a nanohole-boosted electron transport (NBET) antibiofilm concept. Unlike known antibacterial mechanisms (e.g., reactive oxygen species production and cell membrane damage), nanoholes with atomic vacancies and biofilms serve as electronic donors and receptors, respectively, and thus boost the high electron transport capacity between nanomaterials and biofilms. Electron transport effectively destroys the critical components (proteins, intercellularly adhered polysaccharides and extracellular DNA) of biofilms, and the nanoholes also significantly downregulate the expression of genes related to biofilm formation. The anti-infection capacity is thoroughly verified both in vitro (human cells) and in vivo (rat ocular and mouse intestinal infection models), and the nanohole-enabled nanomaterials are found to be highly biocompatible. Importantly, compared with typical antibiotics, nanomaterials are nonresistant and thereby exhibit high potential for use in various applications. As a proof-of-principle demonstration, these findings hold promise for the use of NBET in treatments for pathogenic bacterial infection and antibiotic drug resistance.
The rise of angiosperms strengthened fire feedbacks and improved the regulation of atmospheric oxygen
Authors: Belcher Claire M. , Mills Benjamin J. W. , Vitali Rayanne , Baker Sarah J. , Lenton Timothy M. , Watson Andrew J. 
Published: January 2021

AbstractThe source of oxygen to Earth’s atmosphere is organic carbon burial, whilst the main sink is oxidative weathering of fossil carbon. However, this sink is to insensitive to counteract oxygen rising above its current level of about 21%. Biogeochemical models suggest that wildfires provide an additional regulatory feedback mechanism. However, none have considered how the evolution of different plant groups through time have interacted with this feedback. The Cretaceous Period saw not only super-ambient levels of atmospheric oxygen but also the evolution of the angiosperms, that then rose to dominate Earth’s ecosystems. Here we show, using the COPSE biogeochemical model, that angiosperm-driven alteration of fire feedbacks likely lowered atmospheric oxygen levels from ~30% to 25% by the end of the Cretaceous. This likely set the stage for the emergence of closed-canopy angiosperm tropical rainforests that we suggest would not have been possible without angiosperm enhancement of fire feedbacks.
Preclinical assessment of the efficacy and specificity of GD2-B7H3 SynNotch CAR-T in metastatic neuroblastoma
Authors: Moghimi Babak , Muthugounder Sakunthala , Jambon Samy , Tibbetts Rachelle , Hung Long , Bassiri Hamid , Hogarty Michael D. , Barrett David M. , Shimada Hiroyuki , Asgharzadeh Shahab 
Published: January 2021

AbstractThe ability to utilize preclinical models to predict the clinical toxicity of chimeric antigen receptor (CAR) T cells in solid tumors is tenuous, thereby necessitating the development and evaluation of gated systems. Here we found that murine GD2 CAR-T cells, specific for the tumor-associated antigen GD2, induce fatal neurotoxicity in a costimulatory domain-dependent manner. Meanwhile, human B7H3 CAR-T cells exhibit efficacy in preclinical models of neuroblastoma. Seeking a better CAR, we generated a SynNotch gated CAR-T, GD2-B7H3, recognizing GD2 as the gate and B7H3 as the target. GD2-B7H3 CAR-T cells control the growth of neuroblastoma in vitro and in metastatic xenograft mouse models, with high specificity and efficacy. These improvements come partly from the better metabolic fitness of GD2-B7H3 CAR-T cells, as evidenced by their naïve T-like post-cytotoxicity oxidative metabolism and lower exhaustion profile.
Inherited predisposition to breast cancer in the Carolina Breast Cancer Study
Authors: Walsh Tom , Gulsuner Suleyman , Lee Ming K. , Troester Melissa A. , Olshan Andrew F. , Earp H. Shelton , Perou Charles M. , King Mary-Claire 
Published: January 2021

AbstractThe Carolina Breast Cancer Study (CBCS) phases I–II was a case-control study of biological and social risk factors for invasive breast cancer that enrolled cases and controls between 1993 and 1999. Case selection was population-based and stratified by ancestry and age at diagnosis. Controls were matched to cases by age, self-identified race, and neighborhood of residence. Sequencing genomic DNA from 1370 cases and 1635 controls yielded odds ratios (with 95% confidence limits) for breast cancer of all subtypes of 26.7 (3.59, 189.1) for BRCA1, 8.8 (3.44, 22.48) for BRCA2, and 9.0 (2.06, 39.60) for PALB2; and for triple-negative breast cancer (TNBC) of 55.0 (7.01, 431.4) for BRCA1, 12.1 (4.18, 35.12) for BRCA2, and 10.8 (1.97, 59.11) for PALB2. Overall, 5.6% of patients carried a pathogenic variant in BRCA1, BRCA2, PALB2, or TP53, the four most highly penetrant breast cancer genes. Analysis of cases by tumor subtype revealed the expected association of TNBC versus other tumor subtypes with BRCA1, and suggested a significant association between TNBC versus other tumor subtypes with BRCA2 or PALB2 among African-American (AA) patients [2.95 (1.18, 7.37)], but not among European-American (EA) patients [0.62 (0.18, 2.09)]. AA patients with pathogenic variants in BRCA2 or PALB2 were 11 times more likely to be diagnosed with TNBC versus another tumor subtype than were EA patients with pathogenic variants in either of these genes (P = 0.001). If this pattern is confirmed in other comparisons of similarly ascertained AA and EA breast cancer patients, it could in part explain the higher prevalence of TNBC among AA breast cancer patients.