bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hoary Squash Bees (Eucera pruinosa: Hymenoptera: Apidae) Provide Abundant and Reliable Pollination Services to Cucurbita Crops in Ontario (Canada)
Authors: Willis Chan D Susan , Raine Nigel E 
Published: April 2021

Abstract
The increasing demand for insect-pollinated crops highlights the need for crop pollination paradigms that include all available pollinators. In North America, Cucurbita crops (pumpkin, squash) depend on both wild (solitary and Bombus spp.: Hymenoptera: Apidae) and managed honey bees (Apis mellifera L. 1758: Hymenoptera: Apidae) for pollination. Temporal and spatial differences in abundance may determine which bee taxa are the most important pollinators of Cucurbita crops. We surveyed bees visiting Cucurbita crop flowers on 19 farms over four years (2015–2018) during the crop flowering period (July 1–August 30 from 06:00–12:00). All the farms surveyed had hoary squash bees (Eucera pruinosa (Say, 1867), and most also had some combination of honey bees, bumble bees (Bombus spp.), or other wild bees present on their Cucurbita crop flowers. All four bee taxa were present on about two-thirds of farms. Spatially and temporally, wild bees were more abundant on Cucurbita crop flowers than managed honey bees. Hoary squash bees were the most abundant wild bees, maintaining their abundance relative to other wild bee taxa year-over-year. Male hoary squash bees were both more frequently and consistently seen visiting crop flowers than females in all years. Peak activity of hoary squash bees and bumble bees coincided with the daily crop pollination window, whereas peak activity of honey bees and other wild bees occurred after that window. In addition to elucidating the ecological interactions among wild and managed pollinators on Cucurbita crops, our work provides a novel practical way to evaluate pollinator abundance using a crop-centered benchmark framework.
Does Democratisation Promote Competition? Evidence from Indonesia*
Authors: Hallward-Driemeier Mary , Kochanova Anna , Rijkers Bob 
Published: April 2021

AbstractDoes democratisation promote economic competition? This paper documents that the disruption of political connections associated with Suharto's fall had a modest pro-competitive effect on Indonesian manufacturing industries. Firms with connections to Suharto lost substantial market share following his resignation. Industries in which Suharto family firms had larger market share during his tenure exhibited weak improvements in broader measures of competition in the post-Suharto era relative to industries in which Suharto firms had not been important players.
Neonatal Death in India: Birth Order in a Context of Maternal Undernutrition
Authors: Coffey Diane , Spears Dean 
Published: April 2021

AbstractWe document a novel fact about neonatal death, or death in the first month of life. Globally, neonatal mortality is disproportionately concentrated in India. We identify a large effect of birth order on neonatal mortality that is unique to India: later-born siblings have a steep survival advantage relative to the birth-order gradient in other developing countries. We show that India’s high prevalence of maternal undernutrition and its correlation with age and childbearing can explain this pattern. We find that Indian mothers exit the underweight body mass range at an internationally comparatively high rate as they progress through childbearing careers.
Factors associated with emergency-related diagnosis, time to treatment and type of treatment in 5713 lung cancer patients
Authors: Nilssen Yngvar , Brustugun Odd T , Møller Bjørn 
Published: April 2021

Abstract

Background
International and national differences exist in survival among lung cancer patients. Possible explanations include varying proportions of emergency presentations (EPs), unwanted differences in waiting time to treatment and unequal access to treatment.

Methods
Case-mix-adjusted multivariable logistic regressions the odds of EP and access to surgery, radiotherapy and systemic anticancer treatment (SACT). Multivariable quantile regression analyzed time from diagnosis to first treatment.

Results
Of 5713 lung cancer patients diagnosed in Norway in 2015–16, 37.9% (n = 2164) had an EP before diagnosis. Higher age, more advanced stage and more comorbidities were associated with increasing odds of having an EP (P &lt; 0.001) and a lower odds of receiving any treatment (P &lt; 0.001). After adjusting for case-mix, waiting times to curative radiotherapy and SACT were 12.1 days longer [95% confidence interval (CI): 10.2, 14.0] and 5.6 days shorter (95% CI: −7.3, −3.9), respectively, compared with waiting time to surgery. Patients with regional disease experienced a 4.7-day shorter (Coeff: −4.7, 95% CI:−9.4, 0.0) waiting time to curative radiotherapy when compared with patients with localized disease. Patients with a high income had a 22% reduced odds [odds ratio (OR) = 0.78, 95% CI: 0.63, 0.97] of having an EP, and a 63% (OR = 1.63, 95% CI: 1.20, 2.21) and a 40% (OR = 1.40, 95% CI: 1.12, 1.76) increased odds of receiving surgery and SACT, respectively.

Conclusion
Patients who were older, had advanced disease or increased comorbidities were more likely to have an EP and less likely to receive treatment. While income did not affect the waiting time for lung cancer treatment in Norway, it did affect the likelihood of receiving surgery and SACT.
Acidic pH enhances butyrate production from pectin by faecal microbiota
Authors: Raba Grete , Adamberg Signe , Adamberg Kaarel 
Published: April 2021

ABSTRACT
Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota. In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h. The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0–8.0 at both dilution rates. The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH &gt; 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH &gt;7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species. Increased gas formation was observed at pH &lt; 6.5. Substantially more CO2 was produced at Dlow than at Dhigh (18-29 vs 12–23 mmol per L medium, respectively). Methane was produced only at Dlow and pH &gt; 7, consistent with the simultaneous increased abundance of Methanobrevibacter smithii. Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium. Fermentation of other dietary fibres can be studied using the same approach. The significance of pH should be more emphasized in gut research and diagnostics.
Interacting impact of maternal inflammatory response and stress on the amygdala transcriptome of pigs
Authors: Keever-Keigher Marissa R , Zhang Pan , Bolt Courtni R , Rymut Haley E , Antonson Adrienne M , Caputo Megan P , Houser Alexandra K , Hernandez Alvaro G , Southey Bruce R , Rund Laurie A , Johnson Rodney W , Rodriguez-Zas Sandra L 
Published: April 2021

Abstract
Changes at the molecular level capacitate the plasticity displayed by the brain in response to stress stimuli. Weaning stress can trigger molecular changes that influence the physiology of the offspring. Likewise, maternal immune activation (MIA) during gestation has been associated with behavior disorders and molecular changes in the amygdala of the offspring. This study advances the understanding of the effects of pre- and postnatal stressors in amygdala gene networks. The amygdala transcriptome was profiled on female and male pigs that were either exposed to viral-elicited MIA or not and were weaned or nursed. Overall, 111 genes presented interacting or independent effects of weaning, MIA, or sex (FDR-adjusted P-value &lt;0.05). PIGY upstream reading frame and orthodenticle homeobox 2 are genes associated with MIA-related neurological disorders, and presented significant under-expression in weaned relative to nursed pigs exposed to MIA, with a moderate pattern observed in non-MIA pigs. Enriched among the genes presenting highly over- or under-expression profiles were 24 Kyoto Encyclopedia of Genes and Genomes pathways including inflammation, and neurological disorders. Our results indicate that MIA and sex can modulate the effect of weaning stress on the molecular mechanisms in the developing brain. Our findings can help identify molecular targets to ameliorate the effects of pre- and postnatal stressors on behaviors regulated by the amygdala such as aggression and feeding.
Developmental Transcriptomics Reveals a Gene Network Driving Mimetic Color Variation in a Bumble Bee
Authors: Rahman Sarthok Rasique , Terranova Tatiana , Tian Li , Hines Heather M 
Published: April 2021

AbstractA major goal of evolutionary genetics and evo-devo is to understand how changes in genotype manifest as changes in phenotype. Bumble bees display remarkable color pattern diversity while converging onto numerous regional Müllerian mimicry patterns, thus enabling exploration of the genetic mechanisms underlying convergent phenotypic evolution. In western North America, multiple bumble bee species converge onto local mimicry patterns through parallel shifts of midabdominal segments from red to black. It was previously demonstrated that a Hox gene, Abd-B, is the key regulator of the phenotypic switch in one of these species, Bombus melanopygus, however, the mechanism by which Abd-B regulates color differentiation remains unclear. Using tissue/stage-specific transcriptomic analysis followed by qRT–PCR validation, this study reveals a suite of genes potentially involved downstream of Abd-B during color pattern differentiation. The data support differential genes expression of not only the first switch gene Abd-B, but also an intermediate developmental gene nubbin, and a whole suite of downstream melanin and redox genes that together reinforce the observed eumelanin (black)-pheomelanin (red) ratios. These include potential genes involved in the production of insect pheomelanins, a pigment until recently not thought to occur in insects and thus lacking known regulatory enzymes. The results enhance understanding of pigmentation gene networks involved in bumble bee color pattern development and diversification, while providing insights into how upstream regulators such as Hox genes interact with downstream morphogenic players to facilitate this adaptive phenotypic radiation.
Seismic anisotropy and implications for lithospheric deformation beneath the Ordos Block and surrounding regions
Authors: Liu Jing , Wu Jianping , Wang Weilai , Cai Yan , Fang Lihua 
Published: April 2021

SUMMARYThe Ordos Block is located at the intersection of the Tibetan Plateau, the South China Plate and the North China Craton. The deep deformation of its surrounding areas is geologically complicated. Seismic anisotropy provides information about past and present deformation of the upper mantle and helps to better understand deep deformation processes. We obtained the anisotropic pattern at high resolution within and surrounding the Ordos Block by analysing teleseismic data from ∼710 newly deployed temporary seismic stations based on SKS phases from ∼86 earthquakes. The central and eastern Ordos Block with a relatively thick and rigid lithosphere is characterized by a weak anisotropy. In the western part of the Ordos Block, the fast-wave polarization direction is dominantly NW–SE. We believe the lithosphere in the western part of the Ordos Block may have undergone significant deformation caused by expansion and compression of the Tibetan Plateau. Near the Datong Basin, the delay time is 0.92 s and the fast-wave polarization direction is mainly oriented NW–SE, perpendicular to the NE-directed compression of the Tibetan Plateau and parallel to the basin extension direction. We speculate that this anisotropy is related to the long-range effect of NE compression of the northeastern Tibetan Plateau on the low-velocity upper mantle in this area. The weak anisotropy in the central part of the Shanxi Rift indicates that the thickness and mechanical strength of the high-velocity lithosphere are higher than in the southern and northern regions.
Piezomagnetic fields associated with a dislocation source in a layered elastic medium
Authors: Yamazaki Ken'ichi 
Published: April 2021

SUMMARYThe piezomagnetic effect is defined as a change in magnetization with applied stress. Changes in the geomagnetic field caused by the piezomagnetic effect, referred to as the piezomagnetic field, have been theoretically estimated and compared by previous studies to interpret observed variations in the geomagnetic field. However, the piezomagnetic field estimated in previous studies may not provide an accurate estimation because they ignored spatial variations in elasticity, leading to only a rough approximation of the properties of Earth's crust. In this paper, a semi-analytical procedure for calculating the piezomagnetic field arising from a point dislocation source embedded in a layered elastic medium is derived. Following a well-established method of the vector surface harmonic expansion, all of the governing equations written in partial differential equations in a real domain, together with the linear constitutive law of the piezomagnetic effect, are converted to a set of ordinary differential equations in a wavenumber domain. Equations in the wavenumber domain are solved analytically, and each component of the piezomagnetic field in the real domain is obtained after applying the Hankel transform. By using the derived procedure, the piezomagnetic and displacement fields due to a finite fault with strike-slip, dip-slip, and tensile-opening mechanisms are estimated for media with layered elasticity structures. Results for a finite fault are obtained by integrating the point source solution over the fault plane. The results of the numerical analysis allow the effect of heterogeneities in rigidity on the piezomagnetic effect to be examined and implications for the findings of previous investigations to be drawn. In cases where the moment-release at the dislocation source is fixed, the effect of the rigidity differences between upper and lower layers on the piezomagnetic field is minor even in the case where the Curie point depth is near the source of dislocation. This result is in contrast to a previous study that assumed the Mogi model and suggested that heterogeneities in the horizontal direction may be of importance when combined with layered rigidity structures. A contrast is seen between the piezomagnetic and displacement fields corresponding to models with layered rigidity structures: the piezomagnetic field is roughly proportional to the moment-release on a source fault, whereas the displacement field is proportional to slip or opening of the fault. Provided that the rigidity of the crust increases with increasing depth, the calculated piezomagnetic field is likely to have been underestimated in many earlier studies, which assumed uniform rigidity and a geodetically inverted size of slip.
Health gains and financial protection from human papillomavirus vaccination in Ethiopia: findings from a modelling study
Authors: Portnoy Allison , Sweet Steven , Desalegn Dawit , Memirie Solomon Tessema , Kim Jane J , Verguet Stéphane 
Published: April 2021

AbstractHigh out-of-pocket (OOP) medical expenses for cervical cancer (CC) can lead to catastrophic health expenditures (CHEs) and medical impoverishment in many low-resource settings. There are 32 million women at risk for CC in Ethiopia, where CC screening is extremely limited. An evaluation of the population health and financial risk protection benefits, and their distributional consequences across socioeconomic groups, from human papillomavirus (HPV) vaccination will be critical to support CC prevention efforts in this setting. We used a static cohort model that captures the main features of HPV vaccines and population demographics to project health and economic outcomes associated with routine HPV vaccination in Ethiopia. Health outcomes included the number of CC cases, and costs included vaccination and operational costs in 2015 US dollars over the years 2019–2118 and CC treatment costs over the lifetimes of cohorts eligible for vaccination in Ethiopia. We estimated the household OOP medical expenditures averted (assuming 68% of direct medical expenditures were financed OOP) and cases of CHE averted. A case of CHE was defined as 40% of household consumption expenditures, and the cases of CHE averted depended on wealth quintile, disease incidence, healthcare use and OOP payments. Our analysis shows that, assuming 100% vaccine efficacy against HPV-16/18 and 50% vaccination coverage, routine HPV vaccination could avert up to 970 000 cases of CC between 2019 and 2118, which translates to ∼932 000 lives saved. Additionally, routine HPV vaccination could avert 33 900 cases of CHE. Approximately one-third of health benefits would accrue to the poorest wealth quintile, whereas 50% of financial risk protection benefits would accrue to this quintile. HPV vaccination can reduce disparities in CC incidence, mortality and household health expenditures. This understanding and our findings can help policymakers in decisions regarding targeted CC control efforts and investment in a routine HPV vaccination programme following an initial catch-up programme.
Assessing single-nucleotide polymorphism selection methods for the development of a low-density panel optimized for imputation in South African Drakensberger beef cattle
Authors: Lashmar Simon F , Berry Donagh P , Pierneef Rian , Muchadeyi Farai C , Visser Carina 
Published: April 2021

Abstract
A major obstacle in applying genomic selection (GS) to uniquely adapted local breeds in less-developed countries has been the cost of genotyping at high densities of single-nucleotide polymorphisms (SNP). Cost reduction can be achieved by imputing genotypes from lower to higher densities. Locally adapted breeds tend to be admixed and exhibit a high degree of genomic heterogeneity thus necessitating the optimization of SNP selection for downstream imputation. The aim of this study was to quantify the achievable imputation accuracy for a sample of 1,135 South African (SA) Drakensberger cattle using several custom-derived lower-density panels varying in both SNP density and how the SNP were selected. From a pool of 120,608 genotyped SNP, subsets of SNP were chosen (1) at random, (2) with even genomic dispersion, (3) by maximizing the mean minor allele frequency (MAF), (4) using a combined score of MAF and linkage disequilibrium (LD), (5) using a partitioning-around-medoids (PAM) algorithm, and finally (6) using a hierarchical LD-based clustering algorithm. Imputation accuracy to higher density improved as SNP density increased; animal-wise imputation accuracy defined as the within-animal correlation between the imputed and actual alleles ranged from 0.625 to 0.990 when 2,500 randomly selected SNP were chosen vs. a range of 0.918 to 0.999 when 50,000 randomly selected SNP were used. At a panel density of 10,000 SNP, the mean (standard deviation) animal-wise allele concordance rate was 0.976 (0.018) vs. 0.982 (0.014) when the worst (i.e., random) as opposed to the best (i.e., combination of MAF and LD) SNP selection strategy was employed. A difference of 0.071 units was observed between the mean correlation-based accuracy of imputed SNP categorized as low (0.01 &lt; MAF ≤ 0.1) vs. high MAF (0.4 &lt; MAF ≤ 0.5). Greater mean imputation accuracy was achieved for SNP located on autosomal extremes when these regions were populated with more SNP. The presented results suggested that genotype imputation can be a practical cost-saving strategy for indigenous breeds such as the SA Drakensberger. Based on the results, a genotyping panel consisting of ~10,000 SNP selected based on a combination of MAF and LD would suffice in achieving a &lt;3% imputation error rate for a breed characterized by genomic admixture on the condition that these SNP are selected based on breed-specific selection criteria.
Determination of Free Solanesol Levels in Cigarette Filters by Liquid Chromatography–Mass Spectrometry
Authors: Bravo Cardenas Roberto , Ngac Phuong , Watson Clifford , Valentin-Blasini Liza 
Published: April 2021

Abstract
Solanesol, a naturally occurring constituent of tobacco, has been utilized as a good marker for environmental tobacco smoke particulate and as a noninvasive predictor of mainstream cigarette smoke tar and nicotine intake under naturalistic smoking conditions. A fast and accurate method for measuring free solanesol to assess tobacco smoke exposure is highly desirable. We have developed and validated a new environmentally friendly, high-throughput method for measuring solanesol content in discarded cigarette filter butts. The solanesol deposited in the used filters can be correlated with mainstream smoke deliveries of nicotine and total particle matter to estimate constituent delivery to smokers. A portion of filter material is removed from cigarette butts after machine smoking, spiked with internal standard solution, extracted and quantitatively analyzed using reverse-phase liquid chromatography coupled to a triple-quadrupole mass spectrometer. The new method incorporates a 48-well plate format for automated sample preparation that reduces sample preparation time and solvent use and increases sample throughput 10-fold compared to our previous method. Accuracy and precision were evaluated by spiking known amounts of solanesol on both clean and smoked cigarette butts. Recoveries exceeded 93% at both low and high spiking levels. Linear solanesol calibration curves ranged from 1.9 to 367 µg/butt with a 0.05 µg/butt limit of detection.
Voices of Resettled Refugee Congolese Women: A Qualitative Exploration of Challenges Associated with Resettling in Ohio
Authors: Evans Na’Tasha M , Spates Kamesha , Kirkland Chelsey , Mubikayi Kabasele Cedric 
Published: April 2021

AbstractRefugees, specifically women, experience challenges associated with being resettled in Ohio. Existing research gives little attention to challenges associated with resettling, specifically among Congolese refugee women. Therefore, the purpose of this study is to provide Congolese refugee women an opportunity to narrate the challenges that they face resettling in Ohio. Translator-assisted, face-to-face interviews were conducted among resettled Congolese refugee women who were 18 years of age or older. All participants (n = 20) were receiving assistance from a local resettlement agency at the time of the study. Researchers applied a thematic analysis approach during the data collection and data analysis process. The three overarching themes were financial insufficiency and unemployment, family concerns, and daily experiences of health. Findings from this study provide an increased understanding of the many complex factors affecting resettled Congolese refugee women and provide resettlement agencies with the information to better support them.
Domestic trauma with penile and scrotum skin degloving and testicular avulsion
Authors: Iafrate M , Leone N , Mancini M , Prayer T , Bassetto F , Moro F Dal 
Published: April 2021

Abstract
Traumatic lesions of male external genitalia are certainly less frequent than the other body sites and in the majority of cases they are caused by work accidents in the metalworking environment or by gunshot wounds. We present a rare case of traumatic degloving lesion of the male external genitalia with avulsion of the left testis caused by an accidental fall from the ladder. Reconstructive surgery was carried out in a single procedure, obtaining an excellent esthetic and functional result.