bluuebunny/crossref_metadata_2025_split huggingface dataset:
Convection Initiation Associated With Ambient Winds and Local Circulations Over a Tropical Island in South China
Authors: Zhu Lei , Bai Lanqiang , Chen Guixing , Sun Y. Qiang , Meng Zhiyong 
Published: August 2021

AbstractConvection is highly active on tropical islands. Based on a 3‐year radar climatology over Hainan Island, a mountainous tropical island in South China, we identified a distinct spatial dependence of convection initiation (CI) on the speed of the large‐scale low‐level flow. Convection initiates most prominently over the lee‐side plains in high‐wind conditions while over mountains in low‐wind conditions. Quasi‐idealized numerical simulations suggest that mountain ridges stimulate the formation and growth of horizontal convective rolls (HCRs) over the lee‐side plains in high‐wind conditions. These HCRs subsequently intersect with sea‐breeze fronts, which enhances the low‐level convergence favoring CI on the lee‐side plains of the island. During low‐wind conditions, thermally driven circulations control and favor CI over the mountains during the day. The findings highlight that the response of local circulations to the lower‐troposphere ambient flow and diurnal thermal heating is an important aspect of convection initiation on the island.
Oceanic Supply of Ice‐Nucleating Particles and Its Effect on Ice Cloud Formation: A Case Study in the Arctic Ocean During a Cold‐Air Outbreak in Early Winter
Authors: Inoue Jun , Tobo Yutaka , Taketani Fumikazu , Sato Kazutoshi 
Published: August 2021

AbstractThe relationship between cloud phase, ice‐nucleating particles (INPs), and ocean state is vital in climate system modeling. High wave conditions induced by the Arctic sea‐ice decline and intensified atmospheric forcing over the ice‐free ocean increase dynamical geochemical oceanic mixing and sea spray supply containing organic substances, which could act as INPs promoting formation of lower tropospheric ice‐containing clouds. Here, we show that the surface ocean state regulates INP concentration and ice cloud presence in the boundary layer. Arctic research cruise data from the marginal ice zone in the Chukchi Sea in November 2018 revealed high number concentrations of INPs active greater than °C and ice‐containing clouds under high wave conditions. Chemical analysis revealed high contents of organic carbon and sea salt that coincided with the increased oceanic turbidity. The findings suggest that elevated levels of marine organics will contribute to modification of cloud phase from liquid droplets to ice crystals.
Light People: Tarik Bourouina
Authors: Wang Hui , Liu Xiaoyi 
Published: August 2021

EditorialIn 2015, 195 countries of the United Nations proposed Sustainable Development Goals so as to alleviate the problems of climate change and global pollution. In France, there is a scientist dedicated to contribute providing solutions for above issues by virtue of MEMS, Lab-On-Chip and metamaterials. This expert is Prof. Tarik Bourouina, a Professor of Physics at ESIEE Paris, Université Gustave Eiffel. He devoted himself to the investigations on micro sensors and metamaterials, and kept seeking their applications in the future blueprint of “Sustainable” and “Smart” cities. On the other hand, he has formed an indissoluble bond with Light: Science and Applications (LIGHT) since the very beginning of the journal. He also set up the LIGHT’s Paris office, which is the first LIGHT’s overseas office in Europe. We are much honored to have an opportunity to exclusively communicate with Prof. Tarik Bourouina, who will share his research experience and stories with LIGHT in this interview.
Weak immunogenicity of SARS-CoV-2 vaccine in patients with hematologic malignancies
Authors: Malard Florent , Gaugler Béatrice , Gozlan Joel , Bouquet Lucie , Fofana Djeneba , Siblany Lama , Eshagh Deborah , Adotevi Olivier , Laheurte Caroline , Ricard Laure , Dulery Rémy , Stocker Nicolas , van de Wyngaert Zoe , Genthon Alexis , Banet Anne , Memoli Mara , Ikhlef Souhila , Sestilli Simona , Vekhof Anne , Brissot Eolia , Marjanovic Zora , Chantran Yannick , Cuervo Nancy , Ballot Eric , Morand-Joubert Laurence , Mohty Mohamad 
Published: August 2021

AbstractThis study evaluated the safety and immunogenicity of BNT162b2 vaccine in patients with hematological malignancies. Antibodies blocking spike binding to immobilized ACE-2 (NAb) correlated with anti-Spike (S) IgG d42 titers (Spearman r = 0.865, p < 0.0001), and an anti-S IgG d42 level ≥3100 UA/mL was predictive of NAb ≥ 30%, the positivity cutoff for NAb (p < 0.0001). Only 47% of the patients achieved an anti-S IgG d42 level ≥3100 UA/mL after the two BNT162b2 inocula, compared to 87% of healthy controls. In multivariable analysis, male patients, use of B-cell targeting treatment within the last 12 months prior to vaccination, and CD19+ B-cell level <120/uL, were associated with a significantly decreased probability of achieving a protective anti-S IgG level after the second BNT162b2 inoculum. Finally, using the IFN-γ ELISPOT assay, we found a significant increase in T-cell response against the S protein, with 53% of patients having an anti-S IgG-positive ELISPOT after the second BNT162b2 inoculum. There was a correlation between the anti-S ELISPOT response and IgG d42 level (Spearman r = 0.3026, p = 0.012). These findings suggest that vaccination with two BNT162b2 inocula translates into a significant increase in humoral and cellular response in patients with hematological malignancies, but only around half of the patients can likely achieve effective immune protection against COVID-19.
The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium
Authors: Maurizy Chloé , Abeza Claire , Lemmers Bénédicte , Gabola Monica , Longobardi Ciro , Pinet Valérie , Ferrand Marina , Paul Conception , Bremond Julie , Langa Francina , Gerbe François , Jay Philippe , Verheggen Céline , Tinari Nicola , Helmlinger Dominique , Lattanzio Rossano , Bertrand Edouard , Hahne Michael , Pradet-Balade Bérengère 
Published: August 2021

AbstractThe R2TP chaperone cooperates with HSP90 to integrate newly synthesized proteins into multi-subunit complexes, yet its role in tissue homeostasis is unknown. Here, we generated conditional, inducible knock-out mice for Rpap3 to inactivate this core component of R2TP in the intestinal epithelium. In adult mice, Rpap3 invalidation caused destruction of the small intestinal epithelium and death within 10 days. Levels of R2TP substrates decreased, with strong effects on mTOR, ATM and ATR. Proliferative stem cells and progenitors deficient for Rpap3 failed to import RNA polymerase II into the nucleus and they induced p53, cell cycle arrest and apoptosis. Post-mitotic, differentiated cells did not display these alterations, suggesting that R2TP clients are preferentially built in actively proliferating cells. In addition, high RPAP3 levels in colorectal tumors from patients correlate with bad prognosis. Here, we show that, in the intestine, the R2TP chaperone plays essential roles in normal and tumoral proliferation.
A synthetic building operation dataset
Authors: Li Han , Wang Zhe , Hong Tianzhen 
Published: August 2021

AbstractThis paper presents a synthetic building operation dataset which includes HVAC, lighting, miscellaneous electric loads (MELs) system operating conditions, occupant counts, environmental parameters, end-use and whole-building energy consumptions at 10-minute intervals. The data is created with 1395 annual simulations using the U.S. DOE detailed medium-sized reference office building, and 30 years’ historical weather data in three typical climates including Miami, San Francisco, and Chicago. Three energy efficiency levels of the building and systems are considered. Assumptions regarding occupant movements, occupants’ diverse temperature preferences, lighting, and MELs are adopted to reflect realistic building operations. A semantic building metadata schema - BRICK, is used to store the building metadata. The dataset is saved in a 1.2 TB of compressed HDF5 file. This dataset can be used in various applications, including building energy and load shape benchmarking, energy model calibration, evaluation of occupant and weather variability and their influences on building performance, algorithm development and testing for thermal and energy load prediction, model predictive control, policy development for reinforcement learning based building controls.
A global database of diversified farming effects on biodiversity and yield
Authors: Jones Sarah K. , Sánchez Andrea C. , Juventia Stella D. , Estrada-Carmona Natalia 
Published: August 2021

AbstractWith the Convention on Biological Diversity conference (COP15), United Nations Climate Change Conference (COP26), and United Nations Food Systems Summit, 2021 is a pivotal year for transitioning towards sustainable food systems. Diversified farming systems are key to more sustainable food production. Here we present a global dataset documenting outcomes of diversified farming practices for biodiversity and yields compiled following best standards for systematic review of primary studies and specifically designed for use in meta-analysis. The dataset includes 4076 comparisons of biodiversity outcomes and 1214 of yield in diversified farming systems compared to one of two reference systems. It contains evidence from 48 countries of effects on species from 33 taxonomic orders (spanning insects, plants, birds, mammals, eukaryotes, annelids, fungi, and bacteria) of diversified farming systems producing annual or perennial crops across 12 commodity groups. The dataset presented provides a resource for researchers and practitioners to easily access information on where diversified farming systems effectively contribute to biodiversity and food production outcomes.
Characterization of THSD7A-antibodies not binding to glomerular THSD7A in a patient with diabetes mellitus but no membranous nephropathy
Authors: Reinhard Linda , Thomas Cindy , Machalitza Maya , Lattwein Erik , Weiss Lothar S. , Vitu Jan , Wiech Thorsten , Stahl Rolf A. K. , Hoxha Elion 
Published: August 2021

AbstractMembranous nephropathy (MN) is an autoimmune disease caused by autoantibodies against the podocyte antigens phospholipase A2 receptor 1 (PLA2R1) and thrombospondin type 1 domain containing protein 7A (THSD7A) in 80% and 2–3% of patients, respectively. THSD7A antibodies are considered to be pathogenic and highly specific for MN patients. Using an indirect immunofluorescence test (IIFT) we detected THSD7A-antibodies (titre 1:10) in the serum of a patient with high proteinuria who, however, in the kidney biopsy was diagnosed with diabetic nephropathy and MN was excluded as a possible cause of proteinuria. Different immunofluorescence assays and Western blot techniques using recombinant THSD7A (rTHSD7A) or THSD7A from different human tissues revealed that the circulating THSD7A-autoantibodies were only of the IgG3 subclass. The patient serum reacted exclusively with rTHSD7A and only when the antigen was present in reducing Western blot conditions, or on formaldehyde-fixed cells for the IIFT. Our findings show for the first time the existence of circulating THSD7A-antibodies recognizing denatured/reduced rTHSD7A, which do not react with glomerular THSD7A in vivo and are thus presumptively non-pathogenic. As a consequence, kidney biopsy or Western blot analyses of THSD7A under non-reducing conditions should be performed to confirm the diagnosis of THSD7A-associated MN, especially in cases with low THSD7A-antibody levels in the IIFT.
Stretchable self-tuning MRI receive coils based on liquid metal technology (LiquiTune)
Authors: Motovilova Elizaveta , Tan Ek Tsoon , Taracila Victor , Vincent Jana M. , Grafendorfer Thomas , Shin James , Potter Hollis G. , Robb Fraser J. L. , Sneag Darryl B. , Winkler Simone A. 
Published: August 2021

AbstractMagnetic resonance imaging systems rely on signal detection via radiofrequency coil arrays which, ideally, need to provide both bendability and form-fitting stretchability to conform to the imaging volume. However, most commercial coils are rigid and of fixed size with a substantial mean offset distance of the coil from the anatomy, which compromises the spatial resolution and diagnostic image quality as well as patient comfort. Here, we propose a soft and stretchable receive coil concept based on liquid metal and ultra-stretchable polymer that conforms closely to a desired anatomy. Moreover, its smart geometry provides a self-tuning mechanism to maintain a stable resonance frequency over a wide range of elongation levels. Theoretical analysis and numerical simulations were experimentally confirmed and demonstrated that the proposed coil withstood the unwanted frequency detuning typically observed with other stretchable coils (0.4% for the proposed coil as compared to 4% for a comparable control coil). Moreover, the signal-to-noise ratio of the proposed coil increased by more than 60% as compared to a typical, rigid, commercial coil.
Improved procedure for electro-spinning and carbonisation of neat solvent-fractionated softwood Kraft lignin
Authors: Khan Inam , Hararak Bongkot , Fernando Gerard F. 
Published: August 2021

AbstractIn general, the electro-spinning of lignin requires it to be functionalised and/or blended with synthetic or natural polymers. This paper reports on the use of solvent fractionated lignin-lignin blend to electro-spin BioChoice softwood Kraft lignin. The blend consisted of acetone-soluble and ethanol-soluble lignin in a binary solvent of acetone and DMSO. Solvent fractionation was used to purify lignin where the ash content was reduced in the soluble lignin fractions from 1.24 to ~ 0.1%. The corresponding value after conventional acid-washing in sulphuric acid was 0.34%. A custom-made electro-spinning apparatus was used to produce the nano-fibres. Heat treatment procedures were developed for drying the electro-spun fibres prior to oxidation and carbonisation; this was done to prevent fibre fusion. The lignin fibres were oxidised at 250 °C, carbonised at 1000 °C, 1200 °C and 1500 °C. The cross-section of the fibres was circular and they were observed to be void-free. The longitudinal sections showed that the fibres were not fused. Thus, this procedure demonstrated that solvent fractionated lignin can be electro-spun without using plasticisers or polymer blends using common laboratory solvents and subsequently carbonised to produce carbon fibres with a circular cross-section.
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
Authors: Park Jongjin , Yoon Jiyoung , Kwon Daekee , Han Mi-Jung , Choi Sunmee , Park Slki , Lee Junghyuk , Lee Kiwook , Lee Jaehwan , Lee Seunghee , Kang Kyung-Sun , Choe Sunghwa 
Published: August 2021

AbstractEfforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP and demonstrated that transfection of the pre-assembled ribonucleoprotein of the two chimeric proteins into human or plant cells resulted in greater targeted mutagenesis efficiency up to 600% without noticeable increase in off-target effects. Improved activity of the two fusion proteins should enable editing of the previously hard-to-edit genes and thus readily obtaining the cells with designer traits.
Improving magnetic resonance imaging with smart and thin metasurfaces
Authors: Stoja Endri , Konstandin Simon , Philipp Dennis , Wilke Robin N. , Betancourt Diego , Bertuch Thomas , Jenne Jürgen , Umathum Reiner , Günther Matthias 
Published: August 2021

AbstractOver almost five decades of development and improvement, Magnetic Resonance Imaging (MRI) has become a rich and powerful, non-invasive technique in medical imaging, yet not reaching its physical limits. Technical and physiological restrictions constrain physically feasible developments. A common solution to improve imaging speed and resolution is to use higher field strengths, which also has subtle and potentially harmful implications. However, patient safety is to be considered utterly important at all stages of research and clinical routine. Here we show that dynamic metamaterials are a promising solution to expand the potential of MRI and to overcome some limitations. A thin, smart, non-linear metamaterial is presented that enhances the imaging performance and increases the signal-to-noise ratio in 3T MRI significantly (up to eightfold), whilst the transmit field is not affected due to self-detuning and, thus, patient safety is also assured. This self-detuning works without introducing any additional overhead related to MRI-compatible electronic control components or active (de-)tuning mechanisms. The design paradigm, simulation results, on-bench characterization, and MRI experiments using homogeneous and structural phantoms are described. The suggested single-layer metasurface paves the way for conformal and patient-specific manufacturing, which was not possible before due to typically bulky and rigid metamaterial structures.
A distinct negative leader propagation mode
Authors: Scholten O. , Hare B. M. , Dwyer J. , Liu N. , Sterpka C. , Kolmašová I. , Santolík O. , Lán R. , Uhlíř L. , Buitink S. , Corstanje A. , Falcke H. , Huege T. , Hörandel J. R. , Krampah G. K. , Mitra P. , Mulrey K. , Nelles A. , Pandya H. , Rachen J. P. , Trinh T. N. G. , ter Veen S. , Thoudam S. , Winchen T. 
Published: August 2021

AbstractThe common phenomenon of lightning still harbors many secrets such as what are the conditions for lightning initiation and what is driving the discharge to propagate over several tens of kilometers through the atmosphere forming conducting ionized channels called leaders. Since lightning is an electric discharge phenomenon, there are positively and negatively charged leaders. In this work we report on measurements made with the LOFAR radio telescope, an instrument primarily build for radio-astronomy observations. It is observed that a negative leader rather suddenly changes, for a few milliseconds, into a mode where it radiates 100 times more VHF power than typical negative leaders after which it spawns a large number of more typical negative leaders. This mode occurs during the initial stage, soon after initiation, of all lightning flashes we have mapped (about 25). For some flashes this mode occurs also well after initiation and we show one case where it is triggered twice, some 100 ms apart. We postulate that this is indicative of a small (order of 5 km$$^2$$

2

) high charge pocket. Lightning thus appears to be initiated exclusively in the vicinity of such a small but dense charge pocket.
Evaluation of two highly effective lipid-lowering therapies in subjects with acute myocardial infarction
Authors: Klassen Aline , Faccio Andrea Tedesco , Picossi Carolina Raissa Costa , Derogis Priscilla Bento Matos Cruz , dos Santos Ferreira Carlos Eduardo , Lopes Aline Soriano , Sussulini Alessandra , Cruz Elisa Castañeda Santa , Bastos Rafaela Tudela , Fontoura Stefanie Caroline , Neto Antonio Martins Figueiredo , Tavares Marina Franco Maggi , Izar Maria Cristina , Fonseca Francisco Antonio Helfenstein 
Published: August 2021

AbstractFor cardiovascular disease prevention, statins alone or combined with ezetimibe have been recommended to achieve low-density lipoprotein cholesterol targets, but their effects on other lipids are less reported. This study was designed to examine lipid changes in subjects with ST-segment elevation myocardial infarction (STEMI) after two highly effective lipid-lowering therapies. Twenty patients with STEMI were randomized to be treated with rosuvastatin 20 mg QD or simvastatin 40 mg combined with ezetimibe 10 mg QD for 30 days. Fasting blood samples were collected on the first day (D1) and after 30 days (D30). Lipidomic analysis was performed using the Lipidyzer platform. Similar classic lipid profile was obtained in both groups of lipid-lowering therapies. However, differences with the lipidomic analysis were observed between D30 and D1 for most of the analyzed classes. Differences were noted with lipid-lowering therapies for lipids such as FA, LPC, PC, PE, CE, Cer, and SM, notably in patients treated with rosuvastatin. Correlation studies between classic lipid profiles and lipidomic results showed different information. These findings seem relevant, due to the involvement of these lipid classes in crucial mechanisms of atherosclerosis, and may account for residual cardiovascular risk.Randomized clinical trial: ClinicalTrials.gov, NCT02428374, registered on 28/09/2014.
Parallel LC shaped metamaterial resonator for C and X band satellite applications with wider bandwidth
Authors: Faruque Mohammad Rashed Iqbal , Siddiky Air Mohammad , Ahamed Eistiak , Islam Mohammad Tariqul , Abdullah Sabirin 
Published: August 2021

AbstractThe electromagnetic properties of the metal based dielectric in the field of millimeter and sub-millimeter technology attracts a new era for innovation. In this research work, we have introduced a parallel LC shaped metamaterial resonator with wider bandwidth. The negative refractive index for two resonant frequencies is located from the negative permittivity from 5.1 to 6.3, 10.4 to 12.9 GHz, where the negative refractive index is located from 5.4 to 6.3 and 10.5 to 13.5 GHz. The electromagnetic wave polarizing in the proposed structure with parallel LC shaped metallic structure shows a fascinating response of wider bandwidth for the external electric and magnetic field. This paper focuses on the design of conducting layer for the suggested design with the parallel metallic arm for analysing the mutual coupling effect of the scattering response where the sub-branch in metallic design is shown more resonant frequencies with the enhancement of the compactness. This proposed structure is analysed with different metallic arrangements and array structures for different boundary conditions.
CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)
Authors: Nanasato Yoshihiko , Mikami Masafumi , Futamura Norihiro , Endo Masaki , Nishiguchi Mitsuru , Ohmiya Yasunori , Konagaya Ken-ichi , Taniguchi Toru 
Published: August 2021

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.