bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microfluidic deep mutational scanning of the human executioner caspases reveals differences in structure and regulation
Authors: Roychowdury Hridindu , Romero Philip A. 
Published: June 2021

AbstractThe human caspase family comprises 12 cysteine proteases that are centrally involved in cell death and inflammation responses. The members of this family have conserved sequences and structures, highly similar enzymatic activities and substrate preferences, and overlapping physiological roles. In this paper, we present a deep mutational scan of the executioner caspases CASP3 and CASP7 to dissect differences in their structure, function, and regulation. Our approach leverages high-throughput microfluidic screening to analyze hundreds of thousands of caspase variants in tightly controlled in vitro reactions. The resulting data provides a large-scale and unbiased view of the impact of amino acid substitutions on the proteolytic activity of CASP3 and CASP7. We use this data to pinpoint key functional differences between CASP3 and CASP7, including a secondary internal cleavage site, CASP7 Q196 that is not present in CASP3. Our results will open avenues for inquiry in caspase function and regulation that could potentially inform the development of future caspasespecific therapeutics.
Intranasal administration of a monoclonal neutralizing antibody protects mice against SARS-CoV-2 infection
Authors: Halwe Sandro , Kupke Alexandra , Vanshylla Kanika , Liberta Falk , Gruell Henning , Zehner Matthias , Rohde Cornelius , Krähling Verena , Gellhorn-Serra Michelle , Kreer Christoph , Klüver Michael , Sauerhering Lucie , Schmidt Jörg , Cai Zheng , Han Fei , Young David , Yang Guangwei , Widera Marek , Koch Manuel , Werner Anke , Kämper Lennart , Becker Nico , Marlow Michael S , Eickmann Markus , Ciesek Sandra , Schiele Felix , Klein Florian , Becker Stephan 
Published: June 2021

AbstractDespite recent availability of vaccines against severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), there is an urgent need for specific anti-SARS-CoV-2 drugs. Monoclonal neutralizing antibodies are an important drug class in the global fight against the SARS-CoV-2 pandemic due to their ability to convey immediate protection and their potential to be used as both, prophylactic and therapeutic drugs. Clinically used neutralizing antibodies against respiratory viruses are currently injected intravenously, which can lead to suboptimal pulmonary bioavailability and thus to a lower effectiveness.Here we describe DZIF-10c, a fully human monoclonal neutralizing antibody that binds the receptor-binding domain of SARS-CoV-2 spike protein. DZIF-10c displays an exceptionally high neutralizing potency against SARS-CoV-2 and retains activity against the variants of concern B.1.1.7 and B.1.351. Importantly, not only systemic but also intranasal application of DZIF-10c abolished presence of infectious particles in the lungs of SARS-CoV-2 infected mice and mitigated lung pathology. Along with a favorable pharmacokinetic profile, these results highlight DZIF-10c as a novel human SARS-CoV-2 neutralizing antibody with high in vitro and in vivo antiviral potency. The successful intranasal application of DZIF-10c paves the way for clinical trials investigating topical delivery of anti-SARS-CoV-2 antibodies.Significance StatementMonoclonal neutralizing antibodies are important in the global fight against the SARS-CoV-2 pandemic due to their ability to convey immediate protection. However, their intravenous application might lead to suboptimal bioavailability in the lung. We here precisely characterize a new monoclonal neutralizing antibody (DZIF-10c) that binds to the receptor binding domain of the spike protein of SARS-CoV-2. DZIF-10c neutralizes SARS-CoV-2 with exceptionally high potency and maintains activity against circulating variants of concern. The antibody has a favorable pharmacokinetic profile and protects mice from SARS-CoV-2 infection. Importantly, we show that intranasal administration of DZIF-10c generates protective efficacy. These results not only identify DZIF-10c as a novel highly potent neutralizing antibody, but further pave the way for a topical application of anti-SARS-CoV-2 antibodies.
Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
Authors: Wang Jiarui , Han Mengting , Roy Anish R. , Wang Haifeng , Möckl Leonhard , Zeng Leiping , Moerner W. E. , Qi Lei S. 
Published: June 2021

SUMMARYThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects.
Corrosion behavior of steel bar embedded in high-ductility cementitious composites under the coupled action of dry–wet cycles and chloride attack
Authors: Guo Li-Ping , Chai Li-Juan , Xu Yan-Hui , Ding Cong , Cao Yuan-Zhang 
Published: June 2021

Purpose
High-ductility cementitious composites (HDCC) have an excellent crack controlled capacity and corrosion resistance capacity, which has a promising application in structure engineering under harsh environment. The purpose of this study is to explore the corrosion mechanism of steel bar in HDCC.

Design/methodology/approach
Intact and the pre-cracked HDCC specimens under the coupled action of different dry–wet cycles and chloride attack were designed, and intact normal concrete (NC) was also considered for comparison. Corrosion behavior of a steel bar embedded in HDCC was analyzed by an electrochemical method, a chloride permeability test and X-ray computed tomography.

Findings
Steel corrosion probability is related to the chloride permeability of the HDCC cover, and the chloride permeability resistance of HDCC is better than that of NC. Besides, crack is the key factor affecting the corrosion of steel bars, and the HDCC with narrower cracks have a lower corrosion rate. Slight pitting occurs at the crack tips. In addition, the self-healing products and corrosion products fill up the cracks in HDCC, preventing the external aggressive ions from entering and thereby decreasing the steel corrosion rate.

Originality/value
HDCC has a superior corrosion resistance than that of NC, effects of variable crack width on corrosion behavior of steel bar in HDCC under the coupled actions of different dry–wet cycles and chloride attack are investigated, which can provide the guide for the design application of HDCC material in structure engineering exposed to marine environment.
Impact of thriving at work on eustress and distress: career growth as mediator
Authors: Um-e-Rubbab Um-e-Rubbab , Faiz Shazia , Safdar Samyia , Mubarak Namra 
Published: June 2021

Purpose
Thriving at work can affect eustress and distress differently, so the main purpose of this study is to determine the impact of thriving at work on stress and to extend the existing literature on stress by examining the mediating mechanism of career growth, which is one of the functions of human resource development, in the relationship between thriving at work and stress. Person environment fit theory is used to explain the framework.

Design/methodology/approach
Data were collected from 179 employees of the hospitality sector of Pakistan to assess the impact of thriving at work on eustress and distress through the mediation of career growth. Structural equation modeling was used to test the hypotheses.

Findings
Results revealed thriving at work is positively related to eustress whereas it is negatively related to distress, and there is a positive association between thriving and career growth. Furthermore, career growth appeared as an effective explanatory mechanism for relationships between thriving at work and stress. Implications for managers are also discussed.

Originality/value
This study encompasses both positive and negative stressors. There are lots of studies available that examine thriving at work and stress, but the present study aims to examine the impact of thriving on both aspects of stress in the presence of career growth as the mediator in the hospitality sector of Pakistan. It also opens new avenues for research on P-E fit theory to gain benefit from the eustress of employees.
Humanitarian supply chain management: modeling the pre and post-disaster relief operations
Authors: Agarwal Sachin , Kant Ravi , Shankar Ravi 
Published: June 2021

Purpose
This study proposed a mathematical model for decision-making in the pre- and post-disaster phases. This research aims to develop a mathematical model for three important fields in the context of humanitarian logistics; stock prepositioning, facility location and evacuation planning in the humanitarian supply chain (HSC) network design.

Design/methodology/approach
This study applied three optimization techniques; classical approach (CA), pattern search algorithm (PSA) and Genetic Algorithm (GA) to solve the proposed mathematical model. The proposed mathematical model attempts to minimize the total relief items supply chain cost and evacuation chain cost of the HSC. A real case study of cyclone Fani, 2019 in Orissa, India is applied to validate the proposed mathematical model and to show the performance of the model.

Findings
The results demonstrate that heuristic approach; PSA performs better and optimal solutions are obtained in almost all the cases as compared to the GA and CA.

Research limitations/implications
This study is limited to deterministic demands in the affected regions, and different scenarios of the disaster events are not considered.

Social implications
The finding reveals that the proposed model can help the humanitarian stakeholders in making decisions on facility location, relief distribution and evacuation planning in disaster relief operations.

Originality/value
The results of this study may offer managerial insights to practitioners and humanitarian logisticians who are engaged in HSC implementation.
The exploration of effect of financial performance to the public welfare
Authors: Habbe Abdul Hamid 
Published: June 2021

PurposeThe purpose of this paper is to test the effects of the financial performance of local governments to the level of welfare in 25 city/regency in South Sulawesi during 2009–2014.Design/methodology/approachThe financial performance is measured by the rate of local autonomy, the effectiveness of local own-source revenue, budget harmony and budget absorption, while the welfare society measured by the Human Development Index (HDI), unemployment and poverty level.FindingsThe regression analysis showed that the performance of region autonomy proved to increase the HDI over the next year and to reduce the poverty rate in two and three years ahead, however, has no correlation with the unemployment. The effectiveness of local own-source revenue can lower unemployment at two and three years ahead but failed to increase the HDI and to reduce poverty. Harmony of spending also neglected to raise the HDI and to reduce the level of unemployment although it can alleviate poverty. The level of budget absorption can improve HDI and reduce the unemployment at two and three years ahead, but failed to lower poverty. Expenditure harmony and budget absorption failed to moderate the relationship between local autonomy, the effectiveness of local own-source revenue and all measurement of welfare, while the expenditure harmony able to moderate the relationship between the effectiveness of local own-source revenue and HDI.Originality/valueTo the best of authors’ knowledge, no previous study has comprehensively studied the effects of level of regionality autonomy and effectivity of local own-source revenue to public welfare, and the moderation effect of Expenditure harmony, budget absorption in relationship between financial performance of local government to public welfare, especially in Indonesia.
Organization cross domain interventions managing strategies and employee outcomes: a role of work–family balance
Authors: Bashir Mohsin , Bhutta M. Khurrum S. , Bari Muhammad Waseem , Saleem Ammara , Tanveer Yasir 
Published: June 2021

Purpose
Although an emerging field in work and family literature, organization cross domain intervention managing strategies (CDIMS) is an under-researched area. This study aims to investigate whether organization non-monetary CDIMS (control over work hours and supervisor support) have an impact on employee outcomes via the mediating effect work–family balance (WFB).

Design/methodology/approach
Data for the study have been gathered in two distinct surveys from 300 employees working at the managerial level in various manufacturing companies of Pakistan with a seven-week time interval to reduce common method variance. Data were collected during January and March 2020. Confirmatory factor analysis has been performed before testing the mediated model.

Findings
Organization non-monetary CDIMS i.e. control over work hours and supervisor support has been associated positively with WFB, which has further mediated the relationship between workplace non-monetary CDIMS and employee professional outcomes.

Research limitations/implications
Both supervisor support and control over work hours improve employee outcomes by experiencing the satisfaction with WFB. It has been recommended that organizations embed cross-domain interventions in their job design to benefit all employees impartially. Thus, all employees can enjoy better WFB and show positive work behaviors.

Originality/value
This study demonstrates the effectiveness of non-monetary organization CDIMS in enhancing the experience of employee’s WFB and improving their professional outcomes.
Improving professional service operations: action research in a law firm
Authors: Balthu Krishna Chaitanya , Clegg Ben 
Published: June 2021

PurposeThe purpose of this paper is to demonstrate how action research-based interventions can effect change in a complex and challenging professional service environment (Lewis and Brown, 2012). This paper presents a successful way to do this. First, by eliciting factors for change driven by deregulation in the United Kingdom’s (UK) legal service sector (Falconer, 2005). Second, by designing and implementing context-sensitive change in a selected legal service firm.Design/methodology/approachThis research adopts a participatory action research methodology involving the use of systems thinking (namely the PrOH modelling methodology) to design suitable interventions and catalyse change.FindingsThis study has generated new knowledge on three fronts–to the legal service operations, to methodology and to the intellectual framework used for abductive reasoning (Checkland and Poulter, 2006). Lessons are transferable to wider professional service operations research. Findings indicate, despite traditional challenges of delivering typical professional services, there is potential for rationalising processes and service delivery commodification, mainly in the low volume, high variety legal service typology (Silvestroet al., 1992).Research limitations/implicationsThis research uses data from an in-depth study of a single organisation.Practical implicationsThis research helped legal service professionals to improve overall efficiency and effectiveness and create new management tools.Social implicationsThis research could help improve legal service operations and make them more accessible.Originality/valueThis research applies a novel, systems thinking based methodology for the first time in a complex professional service operations environment leading to three-fold contributions in the areas of practice, theory and methodology. The paper uses a change management framework (the Change Kaleidoscope), a soft systems methodology (PrOH modelling) and applies these to legal services.
Applying SDGs as a systematic approach for incorporating sustainability in higher education
Authors: Rajabifard Abbas , Kahalimoghadam Masoud , Lumantarna Elisa , Herath Nilupa , Hui Felix Kin Peng , Assarkhaniki Zahra 
Published: June 2021

Purpose
The achievement of the sustainable development goals (SDGs) for all communities and jurisdictions require a comprehensive roadmap that encompasses all dimensions of data infrastructure, social, economic, environmental and governance ecosystems. With this in mind, this paper aims to establish the link between the curriculum and intended learning outcomes of undergraduate and postgraduate subjects offered by the University and sustainability. This study is a part of a wider university strategy to embed sustainability knowledge and values in the university curricula. The 17 SDGs developed as a part of the 2030 Agenda for Sustainability Development was used as tool to measure and map how the subjects are linked with sustainability.

Design/methodology/approach
To incorporate sustainability into the curriculum, this paper developed an interdisciplinary approach for analysing the interconnection between the SDGs, the expected subject learning points and the relevant aspects of sustainability (geospatial information, the legal, policies and institutional components). As part of the approach, in the first phase of the study, qualitative data were collected through a review of published information on the SDGs and the content of the subjects available in the subject handbook. Subject codes were assigned to the keywords and key phrases extracted from the SDGs and the subject content, and then compared and matched to establish the link between the subjects and the SDGs. Six university schools offering over 2,157 subjects were investigated. In the second phase, a survey was conducted involving subject coordinators with the purpose of validating the findings of the first phase and determining the strength of the linkages between the subjects and the SDGs. In the third phase, a plugin was designed to be used in the digital twin platform developed in the UoM, allowing visualisation of the research outcomes.

Findings
Based on the interim findings, it was found that some subjects within the schools are linked to more than one SDG. However, not all of the subjects within the schools can be linked to the SDGs. There is a scope of improvement for embedding sustainability in more subjects within the schools. Some of the schools were also found to have weak linkages with sustainability, which demonstrate the challenge in technical subjects in linking their subject contents with sustainability.

Originality/value
This study provides a methodology which enables the integration of sustainability into current state of the curricula at the university to be established. Further, with the advancement of geospatial technology and new visualisation opportunities through the use of the digital twin platform provides capabilities to communicate the outcomes of sustainability and involvement of each faculties and departments more effectively to the university community and wider stakeholders.
Factors affecting user acceptance for NFC mobile wallets in the U.S. and Korea
Authors: Shin Seungjae , Lee Won-Jun 
Published: June 2021

Purpose
The purpose of this paper is to identify the factors affecting user acceptance for NFC mobile wallets in both Korean and US markets.

Design/methodology/approach
The proposed model extends the UTAUT2 model with new constructs of credibility (CR) and service smartness (SS). This study was analyzed using partial least square structural equation modeling on data collected from 701 college students between the ages of 18 and 29.

Findings
The results of this study demonstrate that performance expectancy (PE), effort expectancy (EE), CR, SS and habit (HB) have strong positive relationships with a user’s behavioral intention to use NFC mobile wallets. Comparing the results of the USA and South Korea, there are different results regarding PE and CR.

Research limitations/implications
This study shows that all factors except social influence (SI) have significant positive relationships with the intention to adopt NFC mobile wallets: Among the original UTUAT2 factors, PE, EE and HB are important determinants of NFC mobile wallet adoption and the new constructs, CR and SS, are significant determinants that influence BI. However, the target respondents are limited to college students of South Korea and the USA Thus, caution should be used when applying the results of this study towards less ICT developed countries and towards different age groups.

Practical implications
This study provides multiple practical contributions. First, this study emphasizes HB as the strongest factor for adopting NFC mobile wallets in both South Korea and the USA Second, this study also highlights the importance of SS. Third, this study reveals that SI is not associated with the adoption of NFC mobile wallets. Fourth, nationality differences between the USA and South Korea account for the differences in consumer behaviors.

Originality/value
This study has two main contributions: First, this study introduces a modified UTAUT2 model with two new variables (CR and SS) useful for NFC mobile wallets. Second, this study compares the results of partial least square structured equation models (PLS-SEM) of the two nationality groups, South Korea and the USA
A framework model for AEC/FM knowledge in adaptive reuse projects
Authors: Hamida Mohammad B. , Hassanain Mohammad A. 
Published: June 2021

PurposeThis paper aims to present a generic lifecycle framework model for guiding architects, engineers, contractors and facilities managers (AEC/FM) practitioners on the effective implementation of adaptive reuse projects.Design/methodology/approachA mixed approach of qualitative and quantitative techniques was followed in the development of the framework model. A literature review was conducted to comprehend the processes involved in adaptive reuse projects. In total, 90 AEC/FM practitioners were surveyed to identify the current practices in these projects. A generic framework model was then developed to standardize the processes involved, using integration definition for function modeling process modeling methodology. Face-to-face interviews with a targeted group of 30 AEC/FM practitioners were conducted, to validate the developed framework model, by assessing the importance and the frequency of implementing each function in the developed framework model.FindingsThe framework model consisted of four sequential processes, namely, assess the feasibility of the adaptive reuse project, design the adaptive reuse project, construct the adaptive reuse project and operate and maintain the adaptive reuse project. The validation confirmed the importance of all the framework functions and the frequency of their implementation.Originality/valueThis research contributes to the literature and the AEC/FM professions, through developing a lifecycle and knowledge-oriented framework model for building adaptive reuse. The framework presents clear documentation of adaptive reuse processes. Thus, it holds the potential of endeavoring on adaptive reuse projects to be more efficient.