bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical evaluation of direct anterior approach total hip arthroplasty for severe developmental dysplasia of the hip
Authors: Liu Zaiyang , Bell Courtney D. , Ong Alvin C. , Zhang Jun , Li Jie , Zhang Yuan 
Published: April 2021

AbstractIt is challenging to treat developmental dysplasia of the hip (DDH) classified Crowe III-IV using direct anterior approach (DAA) total hip arthroplasty (THA), and very little is known on its outcome. This study aimed to investigate the clinical result in this defined disorder with DAA versus posterolateral approach. Twenty-three consecutive hips with Crowe III-IV DDH who underwent DAA were retrospectively evaluated from 2016 through 2018. Outcomes were primarily assessed by HHS, WOMAC, and SF-12 physical scales. The second evaluations included leg length discrepancy, hip muscle strength, radiographic review, complications, and limp recovery. Results were compared to a control cohort of 50 hips underwent posterolateral THA concurrently within the observational period. At last follow-up (DAA 28.5 months; PLA 39.0 months), the mean increase of the HHS for DAA was 48.2 and 30.3 for PLA (p = 0.003). The improvement in WOMAC score in DAA cohort was 15.89 higher that of the PLA cohort after adjusting preoperative difference [R2 = 0.532, P = 0.000, 95% CI (10.037, 21.735)]. DAA had more rapid recovery of hip abductor strength at 1-month (p = 0.03) and hip flexor strength at 3 months (p = 0.007) compared to PLA. No significant differences were found in the radiographic analysis with the exception of increased acetabular anteversion in the DAA cohort (p = 0.036). Satisfactory improvement in limp, indicated by the percentage of limp graded as none and mild to the total, was much higher in DAA cohort (97.6%), compared to that of PLA cohort (90.0%, p = 0.032). DAA for high-dislocated dysplasia demonstrate a significant improvement in clinical result comparable to posterolateral approach. Improved clinical outcome in terms of increased HHS and WOMAC scores, rapid recovery of hip abductor and flexor strength, and enhanced limp recovery without an increased risk in complications, could be acquired when the surgeons were specialized in this approach.
RETRACTED ARTICLE: Carvacrol and PPARγ agonist, pioglitazone, affects inhaled paraquat-induced lung injury in rats
Authors: Amin Fatemeh , Memarzia Arghavan , Rad Hamideh Kazemi , Kazerani Hamid Reza , Boskabady Mohammad Hossein 
Published: April 2021

AbstractExposed rats to normal saline and paraquat (PQ) aerosol as control and PQ group, rats exposed to PQ and treated with 20 and 80 mg/kg/day carvacrol, 5 and 10 mg/kg/day pioglitazone, low dose of pioglitazone + carvacrol and 0.03 mg/kg/day dexamethasone (Dexa) for 16 days after the end of PQ exposure were studied (n = 6 in each group). Lung pathological changes, tracheal responsiveness to methacholine and ovalbumin (OVA) as well as transforming growth factor beta (TGF-β) and interleukin (IL)-6 level in the lung tissue homogenize as well as TGF-β, IL-6, oxidant and antioxidant levels oxidant and antioxidants were increased in PQ group (p < 0.01 to p < 0.001). Lung pathological changes, tracheal responsiveness to methacholine and OVA as well as TGF-β, IL-6 oxidant and antioxidant levels were improved in all treated groups except lung pathological changes in treated group with low dose of pioglitazone (p < 0.05 to p < 0.001). The effects of low dose of pioglitazone and carvacrol alone were significantly lower than in the combination group of low dose of pioglitazone + carvacrol (p < 0.05 to p < 0.001). Carvacrol treatment improved inhaled PQ-induced lug injury similar to the effects of dexamethasone. The synergic effect of carvacrol and pioglitazone suggests PPAR-γ receptor mediated effects of carvacrol on inhaled PQ-induced lung injury.
3D cell sheet structure augments mesenchymal stem cell cytokine production
Authors: Bou-Ghannam Sophia , Kim Kyungsook , Grainger David W. , Okano Teruo 
Published: April 2021

AbstractMesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation in three-dimensional (3D) tissue-like microenvironments. Toward this end, this study explored scaffold-free cell sheet technology as a new 3D platform. MSCs cultivated on temperature-responsive culture dishes to a confluent 2D monolayer were harvested by temperature reduction from 37 to 20 °C that induces a surface wettability transition from hydrophobic to hydrophilic. Release of culture-adherent tension induced spontaneous cell sheet contraction, reducing the diameter 2.4-fold, and increasing the thickness 8.0-fold to render a 3D tissue-like construct with a 36% increase in tissue volume. This 2D-to-3D transition reorganized MSC actin cytoskeleton from aligned to multidirectional, corresponding to a cell morphological change from elongated in 2D monolayers to rounded in 3D cell sheets. 3D culture increased MSC gene expression of cell interaction proteins, β-catenin, integrin β1, and connexin 43, and of pro-tissue regenerative cytokines, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10), and increased VEGF secretion per MSC 2.1-fold relative to 2D cultures. Together, these findings demonstrate that MSC therapeutic potency can be enhanced by 3D cell sheet tissue structure.
Long-term patterns of cave-exiting activity of hibernating bats in western North America
Authors: Whiting Jericho C. , Doering Bill , Aho Ken , Rich Jason 
Published: April 2021

AbstractUnderstanding frequency and variation of cave-exiting activity after arousal from torpor of hibernating bats is important for bat ecology and conservation, especially considering white-nose syndrome. In winter from 2011 to 2018, we acoustically monitored, and counted in hibernacula, two species of conservation concern—western small-footed myotis (Myotis ciliolabrum) and Townsend’s big-eared bats (Corynorhinus townsendii)—in 9 caves located in important habitat for these species in western North America. We investigated if cave-exiting activity differed by species, cave, number of hibernating bats, moon phase, and weather variables. Both species exited hibernacula during all winter months, but most activity occurred in March followed by November. Although we counted almost 15 times more Townsend’s big-eared bats during hibernacula surveys, we documented western small-footed myotis exiting caves 3 times more than Townsend’s big-eared bats. Cave-exiting activity increased with increasing number of hibernating bats, but more so for western small-footed myotis. Both species of bats were active during warm weather and low wind speeds. Western small-footed myotis were more active during colder temperatures, higher wind speeds, and greater change in barometric pressure than Townsend’s big-eared bats. Our results provide a long-term dataset of cave-exiting activity after arousal from torpor during hibernation for these species before the arrival of white-nose syndrome.
Adrenergic inhibition facilitates normalization of extracellular potassium after cortical spreading depolarization
Authors: Monai Hiromu , Koketsu Shinnosuke , Shinohara Yoshiaki , Ueki Takatoshi , Kusk Peter , Hauglund Natalie L. , Samson Andrew J. , Nedergaard Maiken , Hirase Hajime 
Published: April 2021

AbstractCortical spreading depolarization (CSD) is a propagating wave of tissue depolarization characterized by a large increase of extracellular potassium concentration and prolonged subsequent electrical silencing of neurons. Waves of CSD arise spontaneously in various acute neurological settings, including migraine aura and ischemic stroke. Recently, we have reported that pan-inhibition of adrenergic receptors (AdRs) facilitates the normalization of extracellular potassium after acute photothrombotic stroke in mice. Here, we have extended that mechanistic study to ask whether AdR antagonists also modify the dynamics of KCl-induced CSD and post-CSD recovery in vivo. Spontaneous neural activity and KCl-induced CSD were visualized by cortex-wide transcranial Ca2+ imaging in G-CaMP7 transgenic mice. AdR antagonism decreased the recurrence of CSD waves and accelerated the post-CSD recovery of neural activity. Two-photon imaging revealed that astrocytes exhibited aberrant Ca2+ signaling after passage of the CSD wave. This astrocytic Ca2+ activity was diminished by the AdR antagonists. Furthermore, AdR pan-antagonism facilitated the normalization of the extracellular potassium level after CSD, which paralleled the recovery of neural activity. These observations add support to the proposal that neuroprotective effects of AdR pan-antagonism arise from accelerated normalization of extracellular K+ levels in the setting of acute brain injury.
Influence of physical activity before and during pregnancy on infant’s sleep and neurodevelopment at 1-year-old
Authors: Nakahara Kazushige , Michikawa Takehiro , Morokuma Seiichi , Ogawa Masanobu , Kato Kiyoko , Sanefuji Masafumi , Shibata Eiji , Tsuji Mayumi , Shimono Masayuki , Kawamoto Toshihiro , Ohga Shouichi , Kusuhara Koichi ,   the Japan Environment and Children’s Study Group, Kamijima Michihiro , Yamazaki Shin , Ohya Yukihiro , Kishi Reiko , Yaegashi Nobuo , Hashimoto Koichi , Mori Chisato , Ito Shuichi , Yamagata Zentaro , Inadera Hidekuni , Nakayama Takeo , Iso Hiroyasu , Shima Masayuki , Kurozawa Youichi , Suganuma Narufumi , Katoh Takahiko 
Published: April 2021

AbstractThe aim of this study was to investigate the association between maternal physical activity (PA) before and during pregnancy and sleep and developmental problems in 1-year-old infants. We used data from a nationwide cohort study in Japan that registered 103,062 pregnancies between 2011 and 2014. Participants were asked about their PA before and during pregnancy, and the sleep and development of their children at the age of 1 year. Maternal PA was estimated using the International Physical Activity Questionnaire and was expressed in METs per week. We defined scores below the cut-off points of the Ages and Stages Questionnaire (ASQ) as abnormal for infant development. Based on the levels of PA before or during pregnancy, the participants were divided into five groups. In mothers with higher PA levels, the risk ratio for bedtime after 22:00 or abnormal ASQ scores in their 1-years-old infants were lower. These associations were observed for PA before and during pregnancy. Higher levels of maternal PA, both before and during pregnancy, may reduce sleep and developmental problems in infants.
Diabetes is associated with greater leg pain and worse patient-reported outcomes at 1 year after lumbar spine surgery
Authors: Nagata Kosei , Nakamoto Hideki , Sumitani Masahiko , Kato So , Yoshida Yuichi , Kawamura Naohiro , Tozawa Keiichiro , Takeshita Yujiro , Nakarai Hiroyuki , Higashikawa Akiro , Iizuka Masaaki , Ono Takashi , Fukushima Masayoshi , Sasaki Katsuyuki , Okazaki Rentaro , Ito Yusuke , Hara Nobuhiro , Doi Toru , Taniguchi Yuki , Matsubayashi Yoshitaka , Tanaka Sakae , Oshima Yasushi 
Published: April 2021

AbstractAlthough patients with diabetes reportedly have more back pain and worse patient-reported outcomes than those without diabetes after lumbar spine surgery, the impact of diabetes on postoperative recovery in pain or numbness in other regions is not well characterized. In this study, the authors aimed to elucidate the impact of diabetes on postoperative recovery in pain/numbness in four areas (back, buttock, leg, and sole) after lumbar spine surgery. The authors retrospectively reviewed 993 patients (152 with diabetes and 841 without) who underwent decompression and/or fixation within three levels of the lumbar spine at eight hospitals during April 2017–June 2018. Preoperative Numerical Rating Scale (NRS) scores in all four areas, Oswestry Disability Index (ODI), and Euro quality of life 5-dimension (EQ-5D) were comparable between the groups. The diabetic group showed worse ODI/EQ-5D and greater NRS scores for leg pain 1 year after surgery than the non-diabetic group. Although other postoperative NRS scores tended to be higher in the diabetic group, the between-group differences were not significant. Diabetic neuropathy caused by microvascular changes may induce irreversible nerve damage especially in leg area. Providers can use this information when counseling patients with diabetes about the expected outcomes of spine surgery.
Data suggest COVID-19 affected numbers greatly exceeded detected numbers, in four European countries, as per a delayed SEIQR model
Authors: Tiwari Sankalp , Vyasarayani C. P. , Chatterjee Anindya 
Published: April 2021

AbstractPeople in many countries are now infected with COVID-19. By now, it is clear that the number of people infected is much greater than the number of reported cases. To estimate the infected but undetected/unreported cases using a mathematical model, we can use a parameter called the probability of quarantining an infected individual. This parameter exists in the time-delayed SEIQR model (Scientific Reports, article number: 3505). Here, two limiting cases of a network of such models are used to estimate the undetected population. The first limit corresponds to the network collapsing onto a single node and is referred to as the mean-$$\beta$$
β
model. In the second case, the number of nodes in the network is infinite and results in a continuum model wherein the infectivity is statistically distributed. We use a generalized Pareto distribution to model the infectivity. This distribution has a fat tail and models the presence of super-spreaders that contribute to the disease progression. While both models capture the detected numbers well, the predictions of affected numbers from the continuum model are more realistic. Our results suggest that affected people outnumber detected people by one to two orders of magnitude in Spain, the UK, Italy, and Germany. Our results are consistent with corresponding trends obtained from published serological studies in Spain, the UK and Italy. The match with limited studies in Germany is poor, possibly because Germany’s partial lockdown approach requires different modeling.
Development of a novel heterologous gene expression system using earthworms
Authors: Akazawa Shin-ichi , Machida Yu , Takeuchi Aya , Wakatsuki Yuka , Kanda Naoki , Kashima Norito , Murayama Hayato 
Published: April 2021

AbstractIn recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-generation animal protein production hosts. These earthworms have been approved as model animals for acute toxicity tests by the Organization for Economic Co-operation and Development, and they have post-translational modification systems. However, so far, none of the studies have used earthworm transfection techniques. Thus, we developed a transfection method for E. fetida and E. andrei using microinjection and electroporation systems. The maximum survival rates and transfection efficiencies were 79.2% and 29.2% for E. fetida, and 95.8% and 50.0% for E. andrei, respectively. Furthermore, human erythropoietin was detected in the transformed earthworm tail fragments using an enzyme-linked immunosorbent assay. These results contribute to the development of a potential earthworm-based novel animal protein production system.
Rifted margins classification and forcing parameters
Authors: Sapin F. , Ringenbach J.-C. , Clerc C. 
Published: April 2021

AbstractRifted margins are the result of the successful process of thinning and breakup of the continental lithosphere leading to the formation of new oceanic lithosphere. Observations on rifted margins are now integrating an increasing amount of multi-channel seismic data and drilling of several Continent-Ocean Transitions. Based on large scale geometries and domains observed on high-quality multi-channel seismic data, this article proposes a classification reflecting the mechanical behavior of the crust from localized to diffuse deformation (strong/coupled to weak/decoupled mechanical behaviors) and magmatic intensity leading to breakup from magma-rich to magma-poor margins. We illustrate a simple classification based on mechanical behavior and magmatic production with examples of rifted margins. We propose a non-exhaustive list of forcing parameters that can control the initial rifting conditions but also their evolution through time. Therefore, rifted margins are not divided into opposing types, but described as a combination and continuum that can evolve through time and space.
Performance of a cardiac lipid panel compared to four prognostic scores in chronic heart failure
Authors: McGranaghan Peter , Saxena Anshul , Düngen Hans-Dirk , Rubens Muni , Appunni Sandeep , Salami Joseph , Veledar Emir , Lacour Philipp , Blaschke Florian , Obradovic Danilo , Loncar Goran , Tahirovic Elvis , Edelmann Frank , Pieske Burkert , Trippel Tobias Daniel 
Published: April 2021

AbstractThe cardiac lipid panel (CLP) is a novel panel of metabolomic biomarkers that has previously shown to improve the diagnostic and prognostic value for CHF patients. Several prognostic scores have been developed for cardiovascular disease risk, but their use is limited to specific populations and precision is still inadequate. We compared a risk score using the CLP plus NT-proBNP to four commonly used risk scores: The Seattle Heart Failure Model (SHFM), Framingham risk score (FRS), Barcelona bio-HF (BCN Bio-HF) and Meta-Analysis Global Group in Chronic Heart Failure (MAGGIC) score. We included 280 elderly CHF patients from the Cardiac Insufficiency Bisoprolol Study in Elderly trial. Cox Regression and hierarchical cluster analysis was performed. Integrated area under the curves (IAUC) was used as criterium for comparison. The mean (SD) follow-up period was 81 (33) months, and 95 (34%) subjects met the primary endpoint. The IAUC for FRS was 0.53, SHFM 0.61, BCN Bio-HF 0.72, MAGGIC 0.68, and CLP 0.78. Subjects were partitioned into three risk clusters: low, moderate, high with the CLP score showing the best ability to group patients into their respective risk cluster. A risk score composed of a novel panel of metabolite biomarkers plus NT-proBNP outperformed other common prognostic scores in predicting 10-year cardiovascular death in elderly ambulatory CHF patients. This approach could improve the clinical risk assessment of CHF patients.
Ancient and present surface evolution processes in the Ash region of comet 67P/Churyumov-Gerasimenko
Authors: Bouquety A. , Jorda L. , Groussin O. , Sejourné A. , Bouley S. , Costard F. 
Published: April 2021

Aims. The Rosetta mission provided us with detailed data of the surface of the nucleus of comet 67P/Churyumov-Gerasimenko. In order to better understand the physical processes associated with the comet activity and the surface evolution of its nucleus, we performed a detailed comparative morphometrical analysis of two depressions located in the Ash region.
Methods. To detect morphological temporal changes, we compared pre- and post-perihelion high-resolution (pixel scale of 0.07–1.75 m) OSIRIS images of the two depressions. We quantified the changes using the dynamic heights and the gravitational slopes calculated from the digital terrain model of the studied area. In particular, we measured seven geometric parameters associated with the two depressions (length, three width values, height, area, and volume) using the ArcGIS software before and after perihelion.
Results. Our comparative morphometrical analysis allowed us to detect and quantify the temporal changes that occurred in two depressions of the Ash region during the last perihelion passage. We find that the two depressions grew by several meters. The area of the smallest depression (structure I) increased by 90 ± 20%, with two preferential growths: one close to the cliff associated with the apparition of new boulders at its foot, and a second one on the opposite side of the cliff. The largest depression (structure II) grew in all directions, increasing in area by 20 ± 5%, and no new deposits have been detected. We interpreted these two depression changes as being driven by the sublimation of ices, which explains their global growth and which can also trigger landslides. The deposits associated with depression II reveal a stair-like topography, indicating that they have accumulated during several successive landslides from different perihelion passages. Overall, these observations bring additional evidence of complex active processes and reshaping events occurring on short timescales (months to years), such as depression growth and landslides, and on longer timescales (decades to millenniums), such as cliff retreat.
Assessment of Retinal Neurodegeneration and Choroidal Thickness in COVID-19 Patients Using Swept-Source OCT Technology
Authors: Cetinkaya Tugba , Kurt Muhammed M. , Akpolat Cetin 
Published: April 2021

Abstract
Purpose To analyze the central macular thickness (CMT), retinal nerve fiber layer thickness (RNFLT), ganglion cell layer thickness (GCLT), and choroidal thickness (ChT) measurements in patients with coronavirus disease 2019 (COVID-19).
Methods The study was conducted cross-sectionally 4 weeks after the completed treatment of COVID-19. The diagnosis of COVID-19 was based on the polymerase chain reaction test and/or clinical and radiological findings. The patients with treated COVID-19 were enrolled in the COVID-19 group; age- and sex-matched healthy participants served as the control group. All patients in the COVID-19 group were hospitalized and treated with favipiravir, moxifloxacin, and heparin without the requirement for intubation. The measurements of CMT, RNFLT (in four quadrants), GCLT (in six sectors of two different boundaries), and ChT (in five locations) were performed by swept-source optical coherence tomography (SS-OCT).
Results Similar visual acuity (p = 0.582) and intraocular pressure (p = 0.766) values were observed between the COVID-19 and control groups. Regarding SS-OCT measurements, all mean CMT, RNFLT (in four quadrants), GCLT (in six sectors of two different boundaries), and ChT (in five locations) values were similar in the COVID-19 and control groups (p > 0.05 for all). In the COVID-19 group, a statistically significant negative correlation was noted between the mean ferritin level and temporal RNFLT (r = − 0.378, p = 0.014) and a positive correlation was observed between the mean ferritin level and nasal RNFLT (r = + 0.371, p = 0.016).
Conclusion SS-OCT measurements showed no retinal neurodegenerative and choroidal thickness alterations in COVID-19 patients. Nonsignificant results might be due to the examination of the patients in the early period of the COVID-19 after the treatment. Therefore, late period OCT measurements should be reviewed with new studies in the future.
Zwanzig-Nagel-Dystrophie der Kindheit
Authors: Makris N. , Megahed M. 
Published: April 2021

ZusammenfassungZwanzig-Nagel-Dystrophie (ZND) der Kindheit ist eine seltene gutartige Nagelkrankheit, bei der alle 20 Nägel betroffen sind. Sie tritt normalerweise idiopathisch auf, kann sich aber mit einer Vielzahl von dermatologischen und nicht dermatologischen Erkrankungen entwickeln. Histopathologisch handelt es sich u. a. um eine Entzündung der Matrix der Nägel, deren Ausmaß zu dem Schweregrad des klinischen Bilds beiträgt. ZND der Kindheit ist i. d. R. eine selbstlimitierende Erkrankung, sodass die Beratung und Beruhigung des Kindes und der Eltern als vernünftige Behandlungsmöglichkeiten gelten. Wir berichten über einen Fall eines 7-jährigen Jungen mit einer 1-jährigen Vorgeschichte von 20-Nagel-Dystrophie ohne eigene Krankheiten oder Haut- oder Nagelerkrankungen in der Familienanamnese.