bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Environmental Selection and Transfer Learning Based Dynamic Multiobjective Optimization Evolutionary Algorithm
Authors: He Qiang , Xiang Zheng , Ren Peng 
Published: March 2021

Abstract
In recent years, the dynamic multiobjective optimization problems (DMOPs), whose major strategy is to track the varying PS (Pareto Optimal Solution, PS) and/or PF (Pareto Optimal Frontier), caused a great deal of attention worldwide. As a promising solution, reusing of “experiences” to establish a prediction model is proved to be very useful and widely used in practice. However, most existing methods overlook the importance of environmental selection in the evolutionary processes. In this paper, we propose a dynamic multiobjective optimal evolutionary algorithm which is based on environmental selection and transfer learning (DMOEA-ESTL). This approach makes full use of the environmental selection and transfer learning technique to generate individuals for a new environment by reusing experience to maintain the diversity of the population and speed up the evolutionary process. As experimental validation, we embed this new scheme in the NSGA-II (non-dominated sorting genetic algorithm). We test the proposed algorithm with the help of six benchmark functions as well as compare it with the other two prediction based strategies FPS (Forward-looking Prediction Strategy, FPS) and PPS (Population Prediction Strategy, PPS). The experimental results testify that the proposed strategy can deal with the DMOPs effectively.
Analysis of Long Intergenic Non-Coding RNAs Transcriptomic Profiling in Skeletal Muscle Growth During Porcine Embryonic Development
Authors: Zhao Wenjuan , Li Zijing , Liu Quan , Xie Su , Li Mengxun , Wang Yuan , Li Changchun 
Published: March 2021

Abstract
Skeletal muscle growth plays a critical role during porcine muscle development stages. Genome-wide transcriptome analysis reveals that thousands of long intergenic non-coding RNAs (lincRNAs) have been identified in various species and implicated as crucial regulator involving in epigenetic regulation. However, comprehensive analysis of lincRNAs in embryonic muscle development stages remain still elusive. Here, we investigated the transcriptome profiles of duroc embryonic muscle tissues from days 33, 65, and 90 of gestation using RNA-seq, there were 228 putative lincRNAs identified. Moreover, these lincRNAs exhibit the characteristics of shorter transcripts length, longer exons, less exon numbers and lower expression level compared with protein-coding transcripts. Differential expression analysis showed that a total of 91 lincRNAs and 2638 mRNAs were differentially expressed. In addition, we also performed quantitative trait locus (QTL) mapping analysis for DE lincRNAs, 113 of 120 DE lincRNAs were localized on 2200 QTLs, we observed many QTLs involved in growth and meat quality traits. Furthermore, we predicted potential target genes of DE lincRNAs in cis or trans regulation. Gene ontology and pathway analysis reveals that potential targets of DE lincRNAs mostly were enriched in the processes and pathways related to tissue development, MAPK signaling pathway, Wnt signaling pathway, TGF-beta signaling pathway and insulin signaling pathway, which involved in skeletal muscle physiological functions. Based on cluster analysis, a co-expression network analysis of DE lincRNAs and their potential target genes indicated that DE lincRNAs highly regulated protein-coding genes associated with skeletal muscle development. In this study, many of the DE lincRNAs identified may play essential roles in pig muscle growth and muscle mass. Our study provides crucial information for exploring further the molecular mechanisms of lincRNAs during skeletal muscle development.
Identification of Molecular Mechanisms Underlying Sex-Associated Differences in the Chronic Obstructive Pulmonary Disease through Bioinformatics Analysis
Authors: Chen Fengshou , Hou Haijia , Han Jie , Tang Bing 
Published: March 2021

Abstract
Background Accumulating evidence suggests the existence sex associated differences in the Chronic Obstructive Pulmonary Disease (COPD). However, limited knowledge exists on the molecular mechanisms underlying sex associated differences in COPD patients. Methods The GSE8581 dataset obtained from the GEO database was used to analyze differentially expressed genes (DEGs). Then enrichment analysis for DEGs were conducted through Metascape. PPI and the hub genes-pathway networks were constructed using the STRING database and Cytoscape software. Finally, the CTD was used to examine the relationships between the hub DEGs and COPD. Results The results revealed that different subsets of DEGs had different characteristics in GO functions and KEGG pathways. Different subsets of hub genes were obtained based on PPI network. The study then constructed the hub genes-pathway network for different subsets to explore the key signaling pathways and hub genes involved. The findings showed that NRAS and RAC1 functioned through “Rap1 signaling pathway” and “PI3K-Akt signaling pathway”, in male COPD patients. On the other hand, “Cholesterol metabolism” was among the important pathways in female COPD patients while the hub genes, APOE and APOC3 functioned through “Cholesterol metabolism”. Moreover, “Ubiquitin mediated proteolysis” and the “p53 signaling pathway” were shown to play more important roles in male COPD patients compared to their female counterparts. Furthermore, CDK2 and UBE2N were the hub genes involved in “p53 signaling pathway” and “Ubiquitin mediated proteolysis”, respectively. Finally the study identified the relationship between the hub genes and COPD in CTD. Conclusions The present study uncovered different molecular mechanisms in COPD patients based on sex. Additionally, distinct pathways and hub genes including NRAS, RAC1, APOE, APOC3, CDK2 and UBE2N were identified in the two genders of COPD patients. Further studies are needed to explore individualized treatment for COPD based on the findings.
Ferulic Acid production by metabolically engineered Escherichia coli
Authors: Lv Huajun , Zhang Ying , Shao Jie , Liu Haili , Wang Yong 
Published: March 2021

Abstract
Ferulic acid (p-hydroxy-3-methoxycinnamic acid, FA) is a natural active substance present in plant cell walls, with antioxidant, anticancer, antithrombotic and other properties; it is widely used in medicine, food, and cosmetics areas. Production of FA by eco-friendly bioprocess is of great potential. In this study, FA was biosynthesized by metabolically engineered Escherichia coli. As the first step, the genes tal (encoding Tyrosine ammonia-lyase, RsTAL) from Rhodobacter sphaeroides, sam5 (encoding p - coumarate 3-hydroxylase, SeSAM5) from Saccharothrix espanaensis and comt (encoding Caffeic acid O-methytransferase, TaCM) from Triticum aestivum were cloned in an operon on the pET plasmid backbone, E. coli strain containing this construction was proved to produce FA from L-tyrosine successfully, and confirmed the function of TaCM as Caffeic acid O-methytransferase. Fermentation results revealed JM109(DE3) as more suitable host cell for FA production than BL21(DE3). After that the genes expression strength of FA pathway were optimized by tuning of promoter strength (T7 promoter or T5 promoter) and copy number (pBR322 ori or p15a ori), and the combination p15a-T5 works best. To further improve FA production, E.coli native pntAB, encoding pyridine nucleotide transhydrogenase, was selected from five NADPH regeneration genes to supplement redox cofactor NADPH for converting p-coumaric acid into caffeic acid in FA biosynthesis process. Sequentially, to further convert caffeic acid into FA, a non-native methionine kinase (MetK from Streptomyces spectabilis) was also over expressed. Based on the flask fermentation data which shows that the engineered E. coli strain produced 212 mg/L of FA with 11.8 mg/L caffeic acid residue, it could be concluded that it is the highest yield of FA achieved by E.coli K-12 strains reported to the best of our knowledge.
Fabrication of Gelatin Microspheres 1 Containing Ammonium Hydrogen Carbonate for the Tunable Release of Herbicide
Authors: Liu He , Zhang Zheng , yang jun , Zang Wanyu , Yang Qing 
Published: March 2021

Abstract
The major challenge in utilizing pesticides lies in identifying the precise application that would improve the efficiency of these pesticides and reduce their environmental and health hazards at the same time. Such application requires the development of specific formulations that enable controlled, stimuli-responsive release of the pesticides. Gelatin is a relatively cheap material characterized by temperature-sensitivity and abundant amino acid groups, which makes it suitable for the storage and controlled release of pesticides. In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyses, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy proposed herein can be used to develop novel, effective herbicide formulations.
A Qualitative Study of the Development and Utilization of Health Facility-based Immunization Microplans in Uganda.
Authors: Kaawa-Mafigiri David , Iradukunda Constance , Atumanya Catherine , Odie Michael , Mancuso Arielle , Tran Nhan , McGrath Janet , Luzze Henry 
Published: March 2021

Abstract
Background: In 2006, Uganda adopted the Reaching Every District strategy with the goal of attaining at least 80% coverage for routine immunizations in every district. The development and utilization of health facility/district immunization microplans is the key to the strategy. A number of reports have shown sub-optimal development and use of microplans in Uganda. This study explores factors associated with sub-optimal development and use of microplans in two districts in Uganda to pinpoint challenges encountered during the microplanning process.Methods: A qualitative study was conducted comparing two districts, Kapchorwa with low immunization coverage and Luwero with high immunization coverage. Data were collected through multilevel observation of health facilities, planning sessions and planning meetings; records review of microplans, micromaps, and meeting minutes; 57 interviews with health workers at the Ministry level and lower cadre health facility workers. Data were analyzed using NVivo 8 qualitative text analysis software. Transcripts were coded, memos and display matrices were developed to examine the process of developing and utilization of microplans, including experiences of health workers (implementers). Results: Three key findings emerged from this study. First, there are significant knowledge gaps about the microplanning process among health workers at all levels (community and district health facility and nationally). Limited knowledge about communities and program catchment areas greatly hinders the planning process by limiting the ability to identify hard-to-reach areas as well as prioritize areas according to need. Secondly, the microplanning tool is bulky and complex. Finally, microplanning is being implemented in the context of already over-tasked health personnel who have to conduct several other activities as part of their daily routines.Conclusions: In order to achieve quality improvement of the Reaching Every District campaign, the microplanning process should be revised. Health workers’ misunderstanding and limited knowledge about the microplanning process, especially at peripheral health facilities, coupled with the complex, bulky nature of the microplanning tool reduces the effectiveness of microplanning in improving routine immunization in Uganda. The study reveals the need to reduce the complexity of the tool and identify ways to train and support workers in the use of the revised tool, including support in incorporating the microplanning process into their busy schedules.
Integrated Genomics Analysis Identifies Recessive Ciliopathy Mutations in Primary Endocardial Fibroelastosis: a Rare Neonatal Cardiomyopathy
Authors: Zhao Yan , Lee-Kai Wang , Eskin Ascia , Kang Xuedong , Fajardo Viviana M. , Mehta Zubin , Pineles Stacy , Schmidt Ryan J. , Nagiel Aaron , Satou Gary , Garg Meena , Federman Myke , Reardon Leigh C. , Lee Steven L. , Biniwale Reshma , Grody Wayne W. , Halnon Nancy , Khanlou Negar , Quintero-Rivera Fabiola , Alejos Juan C. , Nakano Atsushi , Fishbein Gregory A. , Arsdell Glen S. Van , Nelson Stanley F. , Touma Marlin 
Published: March 2021

Abstract
Among neonatal cardiomyopathies, primary endocardial fibroelastosis (pEFE) remains a mysterious disease of the endomyocardium, affecting 1/5000 live births and accounting for 25% of the entire pediatric dilated cardiomyopathy with a devastating course and grave prognosis. To investigate the potential genetic cause that underlies pEFE, we performed integrative genomic analysis, using whole exome sequencing (WES) and RNA-seq in a female infant with confirmed pathological diagnosis of pEFE. Within regions of homozygosity in the proband genome, WES analysis revealed novel parent-transmitted homozygous mutations affecting three genes with known roles in cilia assembly or function. Among them, a novel homozygous variant [c.1938delA] of uncertain significance in ALMS1 was prioritized for functional genomic and mechanistic analysis. Loss of function mutations of ALMS1 have been implicated in Alstrom syndrome (AS) [OMIM 203800], a rare recessive ciliopathy that has been associated with cardiomyopathy. The variant of interest results in a frameshift introducing a premature stop codon. RNA-seq of the proband’s dermal fibroblasts confirmed the impact of novel ALMS1 variant on RNA-seq reads and revealed dysregulated cellular signaling and function, including the induction of epithelial mesenchymal transition (EMT) and activation of TGFβ signaling. ALMS1 loss enhanced cellular migration in patient fibroblasts as well as neonatal cardiac fibroblasts, while ALMS1-depleted cardiomyocytes exhibited enhanced proliferation activity. Herein we present the unique pathological features of pEFE compared to DCM and utilize integrated genomic analysis to elucidate the molecular impact of a novel mutation in ALMS1 gene in an AS case. Our report provides insights into pEFE etiology and suggests, for the first time to our knowledge, ciliopathy as a potential underlying mechanism for this poorly understood and incurable form of neonatal cardiomyopathy.
Data-driven optimization algorithm for economic operation of microgrid based on dynamic Kriging model and two-stage search strategy
Authors: Fan Chaodong , Bo Hou , Qu Xilong , Xiao Leyi , Cheng Fanyong 
Published: March 2021

Abstract
The economic operation optimization of microgrid is an important research topic in the power system. Therefore, this paper proposes a surrogate model particle swarm optimization algorithm based on the global-local search mechanism. Firstly, aiming at the problem that the statistical information of Kriging model is difficult to guarantee the prediction accuracy, the dynamic transformation is carried out to enhance the robustness of the model; secondly, the global-local search mechanism is introduced to make the algorithm fully explore the fitness landscape near the Kriging model after quickly locating the current optimal particle position, so as to achieve the balance of convergence quality and convergence efficiency. The proposed method has been tested on numerous benchmark test functions from two test suites, and the results show that the proposed algorithm has more advantages than other comparison algorithms in optimization accuracy. Finally, simulations are carried out in two operating modes of microgrid islanding and grid-connected, which has verified the effectiveness of the proposed method.
Using Pharmacist-Led Tele-Consultation to Review Patients with Chronic Obstructive Pulmonary Disease
Authors: Tatari Wisam , Sowter Julie , Assi Khaled 
Published: March 2021

Abstract
Background: Remote tele-consultation (TC) is seen as one possible solution to circumvent accessibility issues and the feeling of isolation when individuals suffering with Chronic obstructive pulmonary disease (COPD) are at home. Since COVID-19, shifting clinical care to TC has been accelerated. Objective: A feasibility study was conducted with patients randomized to the intervention, a tele-consultation, or a conventional in-person consultation. The study aimed to inform a future definitive trial to test the impact of pharmacist interventions via TC amongst patients with COPD. Setting: Conducted in a single community pharmacy by one specialist respiratory pharmacist. Method: Participants were >35 years, with clinician diagnosed COPD and able to communicate in English. Participants were randomized (1:1) to receive an in-person consultation (IPC) or TC after spirometry, oximetry, and BMI measurements in-person. Consultations comprised of COPD review, education and medication optimization. After 6-months, all participants were reassessed in-person. Main Outcome Measure: Determining the feasibility of the rate of patient recruitment, retention, acceptability of TC, practicalities and completeness of data collection for outcome measures used to assess COPD control and management. Results : Forty-eight patients from 16 GP practices were recruited over 4-months; 41 (85%) completed the study. At follow-up, 29% of participants in the TC group declared a preference for IPC. Collection of data on all outcomes were completed with no adverse events.Conclusion: The recruitment target was met by employing additional strategies and achieved a satisfactory retention rate. Acceptability of the process of conducting TCs and the completeness of data collection was confirmed.
Water repellent Bi2MoO6 cotton fabric for implementing water-source purification
Authors: Yue Jie , Tang Shaowang , Ge Bo , Wang Min , Ren Guina , Shao Xin 
Published: March 2021

Abstract
As a low-budget, facile-availability and eco-friendly substrate material, cotton fabric after hydrophobic modification has been applied to sewage purification. While, a single superhydrophobic function is unable to decompose toxic water-soluble organic dye. In this paper, pathways are propounded for the simultaneous achievement of the removal of insoluble spilling oil and organic dye contaminants. Particularly, the hydrophobic ZnSnO3 after stearic acid modification and Bi2MoO6 catalysts are introduced into the cotton fabric substrate through solution dip-coating. The durability of the prepared fabric suffers from the acid-base corrosion, thermal treatment and mechanical wear, meanwhile, it still exhibits remarkable water repellent (WCA > 150°) property. Furthermore, the remarkable photocatalytic activity makes it possible for reusable degradation and the primary active species, namely the holes, to be verified by the radicals-capturing experiment.
Pathway of Suspected-COVID-19 Patients From French Emergency Departments During The First Outbreak: A Prospective Controlled Cohort Study
Authors: DOUPLAT marion , GAVOILLE Antoine , SUBTIL Fabien , HAESEBAERT Julie , JACQUIN Laurent , DURAND Guillaume , LEGA Jean- Christophe , PERPOINT Thomas , POTINET Véronique , SIGAL Alain , BERTHILLER Julien , PERRETON Nathalie , TAZAROURTE Karim 
Published: March 2021

Abstract
BackgroundTo describe the clinical characteristics, management, and outcome of patients admitted in French emergency departments (EDs) for COVID-19 suspicion.Methods A prospective, multicenter, observational study was conducted in 4 EDs. Adult patients (≥18 years) admitted in EDs between March 6, 2020 and May 10, 2020, hospitalized, and who were presenting symptoms evocative of COVID-19 were included. The clinical features, management, and prognosis of patients was compared according to their confirmed COVID-19 status.Results A total of 2,686 patients were included, among them 760 (28.3%) were COVID-19 positive. Among the latter, the mean ± SD age was 71.5 ± 16.5 years. A total of 364 (48.0%) positive patients had hypertension, 228 (30.0%) had chronic cardiac disease, 186 (24.5%) had diabetes, 126 (16.6%) were obese, and 114 (15.0%) had chronic respiratory disease. The proportion of patients admitted in intensive care units (ICU) was higher among COVID-19 positive patients (185, 24.3%) compared to COVID-19 negative patients (206, 10.7%; p<0.001) and they required mechanical ventilation more frequently (89, 11.9% vs 37, 1.9%; p<0.001). The median [IQR] length of hospital stay was longer among COVID-19-positive patients (10 [6-15] vs 6 [2-11] days; p<0.001). The in-hospital mortality was significantly higher among COVID-19 positive patients (139, 18.3% vs 149, 7.7%; p<0.001).Conclusions This study suggests that among the COVID-19 suspected patients admitted in EDs and requiring a hospitalization, the management was different according to the confirmed COVID-19 status and required different resources in terms of ICU beds and ventilator support.
Experiment Regarding Magnetic Fields with Gravity
Authors: LEE JONG-HOON 
Published: March 2021

Abstract
We observed the Earth's gravity to produce electricity in the magnetic sea. The gravity generator (GG) generated currents and voltages on the mesoscopic scale when we connected it in the vacuum. Gravity interacts to generate electricity in the Earth’s direction or the opposite direction by the repulsive magnetic force. The ground-based device simulates the potential energy (voltages) between gravity and magnetic seas. The GG increased simulations of gravity quantum effects using different physical set-ups. A trapped gravity was set to behave as free relativistic quantum particles or fluids, which made it possible to measure the particle position as a function of time and study the magnetic sea for different initial superpositions of positive- negative-gravity spinor state. It might explain the relativistic quantum equations, antigravity, and the heating mechanism of the Sun.