bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of seven circRNAs that are repeatedly down-regulated in gastric cancer as a biomarker and their potential miRNA targets
Authors: Guo Qijing , Luo Yushaung , Zhao Linglin , Li Yan , Guo Cuiping , Yang Jie , Zhou Xiaofeng , Ren Dengfeng , Zhao Fuxing , Chen Xiaoqian , Zhang Xin , Dang Shengyan , Shen Xianliang , Chen Siai 
Published: September 2021

Abstract
Objective: This study aimed to screen and identify the differentially-expressed circRNAs (DE-circRNAs) in multiple microarray data sets of gastric cancer (GC), predict their corresponding miRNAs, and explore their regulatory relationship, thereby identifying potential molecular markers for early diagnosis of GC. Results : Seven DE-circRNAs were screened and identified: hsa_circ_0007991, hsa_circ_0013048, hsa_circ_0048607, hsa_circ_0050745, hsa_circ_0054971, hsa_circ_0059802 and hsa_circ_0067934. The host genes of these DE-circRNAs are enriched in cancer-related signaling pathways, including ERb signaling, mTOR signaling, chemokine signaling, and B cell receptor signaling. Moreover, these seven DE-circRNAs were all down-regulated in GC tissues. The 7 miRNAs predicted by DE-circRNAs were expressed in GC tissues, namely hsa-miR-1182, hsa-miR-1225-3p, hsa-miR-1275, hsa-miR-193a-5p, hsa-miR-543, hsa-miR-622 and hsa-miR-630. Among them, miR-193a targets were abundant and highly enriched in cancer-related pathways (ERb signaling and mTOR signaling), which was highly consistent with the enrichment of the host gene where the DEcircRNA was located. These results suggested that circRNA may regulate the occurrence and development of GC through the regulation of the expression of the host genes and miRNA. In clinical samples, DE-circRNAs were less expressed in GC tissues when compared to normal tissues. Conclusion : Seven down-regulated DE-circRNAs and 7 targeted miRNAs were identified in GC, which contributed to the understand of the underlying molecular mechanism of GC and provided a basis for the early diagnosis of GC and the development of targeted drugs. Methods: Four sets of circRNA microarray data and one set of miRNA microarray data in GC and adjacent tissues were downloaded from the Gene Expression Omnibus (GEO) and analyzed using GEO2R tool to identify DE-circRNAs and differentially-expressed miRNAs (DE-miRNAs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the enrichment pathway of host mRNAs of DE-circRNAs. Miranda and TargetScan were used to predict miRNA-binding DE-circRNAs. The predicted DE-circRNA interacting miRNAs were intersected with DE-miRNAs, and KEGG and Reactome analyzed the targeted mRNAs corresponding to the obtained miRNAs. DE-circRNAs in GC tissues and adjacent tissues was verified in clinical samples.
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum
Authors: Foulquier Celine , Rivière Anntoine , Heulot Mathieu , Reis Suzana Dos , Perdu Caroline , Girbal Laurence , Pinault Mailys , Dusséaux Simon , Soucaille Philippe , Meynial-Salles Isabelle 
Published: September 2021

Abstract
Clostridium acetobutylicum is a promising biocatalyst for the production of n-butanol at high yield from renewable resources. Several metabolic strategies have already been developed to increase butanol yields, most often based on carbon pathway redirection. However, it was previously demonstrated that the activities of both ferredoxin-NADP+ reductase and ferredoxin-NAD+ reductase, whose encoding genes remained unknown until this study, were necessary to produce the NADPH and the extra NADH needed for butanol synthesis under solventogenic conditions. Here, we purified, identified and characterized the proteins responsible for both ferredoxin-NADP+ reductase and ferredoxin-NAD+ reductase activities and demonstrated the involvement of the identified enzymes in butanol synthesis through a reverse genetic approach. We further demonstrated the yield of butanol formation was limited by the level of expression of CAC_0764, the ferredoxin-NADP+ reductase encoding gene. The integration of these enzymes into metabolic engineering strategies introduces new opportunities for developing a homobutanologenic C. acetobutylicum strain.
Dysregulation of Grainyhead-like 3 expression causes widespread developmental defects
Authors: Deng Zihao , Butt Tariq , Arhatari Benedicta D. , Darido Charbel , Auden Alana , Swaroop Dijina , Partridge Darren D. , Haigh Katharina , Nguyen Thao , Haigh Jody J. , Carpinelli Marina R. , Jane Stephen 
Published: September 2021

Abstract
The gene encoding the transcription factor, Grainyhead-like 3 (Grhl3), plays critical roles in mammalian development and homeostasis, and these have been uncovered through analysis of loss-of-function models. Grhl3-null embryos exhibit a range of gross phenotypes including a shortened longitudinal axis, thoraco-lumbo-sacral spina bifida and soft-tissue syndactyly. Additional studies reveal that these embryos also exhibit a proliferation/differentiation imbalance in the epidermis. This manifests as a failure in skin barrier formation resulting in peri-natal lethality, and defective wound repair. Conditional inactivation of Grhl3 in the squamous epithelium of adult skin, head and neck tissues, and oesophagus reproduces this proliferation/differentiation imbalance and leads to squamous cell carcinomas. These observations establish GRHL3 as a critical tumour suppressor. Despite these extensive analyses of Grhl3 loss-of-function models, the consequences of gain-of-function of this gene have been difficult to achieve. We have redressed this issue through the generation of a novel mouse model that expresses Grhl3 from a transgene integrated in the Rosa26 locus on an endogenous Grhl3-null background. Expression of the transgene rescues both the neurulation and skin barrier defects of the knockout mice, allowing survival into adulthood. Despite this, the mice are not normal, exhibiting a range of phenotypes attributable to dysregulated Grhl3 expression. In mice homozygous for the transgene, we observe a severe Shaker-Waltzer phenotype associated with hearing impairment. Micro-CT scanning of the cochleae and the vestibular apparatus revealed profound structural alterations underlying these phenotypes. In addition, these mice exhibit other developmental anomalies including hair loss, digit defects and epidermal dysmorphogenesis. These findings indicate that diverse developmental processes display low tolerance to dysregulation of Grhl3.
Major Dietary Patterns in Relation to Chronic Low Back Pain; a Cross- sectional Study from RaNCD Cohort
Authors: Pasdar Yahya , Hamzeh Behrooz , Karimi Sheno , Moradi Shima , Cheshmeh Sahar , Shamsi Mohammad Bagher , Najafi Farid 
Published: September 2021

Abstract
Background

Chronic low back pain (LBP) is the most common musculoskeletal pain that affects a person's daily activities. This present study aimed at evaluating the relationship between major dietary pattern and Chronic LBP.
Methods

This cross- sectional study was applied using data from Ravansar non- communicable diseases (RaNCD) cohort study. Chronic LBP was diagnosed by the RaNCD cohort study physician. Dietary patterns were evaluated by principal component analysis. The three identified dietary patterns included: 1) vegetarian dietary pattern which included vegetables, whole grain, legumes, nuts, olive, vegetable oil, fruits, and fruit juice; 2) high protein diet related to higher adherence to red and white meat, legumes, nuts, and egg; and 3) unhealthy dietary pattern characterized with higher intake of salt, sweet, dessert, hydrogenated fat, soft drink, refined grain, tea, and coffee.
Results

22.5% of participants had chronic LBP Higher following high protein dietary pattern was associated with lower risk of chronic LBP in crude (OR: 0.79, 95% CI: 0.69–0.9) and adjusted model (for age, sex, smoking, drinking, diabetes, physical activity, and body mass index) (OR: 0.84, 95% CI: 0.72–0.97). In addition, after controlling for the mentioned potential confounders, participants in the highest category of unhealthy dietary pattern were higher at risk of chronic LBP compared with those in the lowest category (OR: 1.13, 95% CI: 1.01–1.32).
Conclusions

Higher adherence to high protein diet significantly the decreased risk of chronic LBP prevalence. In addition, we found that following unhealthy dietary pattern was associated with higher risk of chronic LBP.
Gut Microbiota Profiles of Systemic Lupus Erythematosus Patients with Anxiety or Depression
Authors: widhani alvina , Gebrina Meutia , Putranto Rudi , Abdullah Murdani , Rinaldi Ikhwan , Djauzi Samsuridjal , Suyatna Fransiscus D. , Dewi Beti Ernawati , Yasmon Andi , Rahayu Susan 
Published: September 2021

Abstract
Background

Patients with systemic lupus erythematosus (SLE) often experience anxiety and depression. Recent studies have shown involvement of intestinal dysbiosis in SLE and also psychosomatic disorders. However, there are no reports on the gut microbiota profile of patient with both conditions: SLE and anxiety or depression. We aimed to study gut microbiota profiles among SLE patients with gastrointestinal symptoms and anxiety or depression by sequencing V3–V4 region of the 16S rRNA gene from the stool samples.
Results

Of the 41 SLE patients who participated in the study, 53.66% had anxiety and 14.63% had depression. We found a higher proportion of Bacteroidetes and lower diversity indices in patients with anxiety than in those without anxiety. We also found a higher proportion of Bacteroidetes and lower Firmicutes/Bacteroidetes ratios and diversity indices in patients with depression than in those without depression. Moreover, compared to other groups, patients with symptoms of both anxiety and depression had the highest proportion of Bacteroidetes and lowest proportion of Firmicutes, Firmicutes/Bacteroidetes ratios, and diversity indices. Further analysis showed that there was a significant correlation between the proportion of Bacteroides and the anxiety score (r = 0.349; p = 0.03) as well as with lupus activity (r = 0.36; p = 0.02). There was also significant correlation between diversity indices and lupus activity (r= -0.34; p = 0.03 for Chao1 index, r= -0.38; p = 0.01 for Shannon index, and r= -0.33; p = 0.03 for richness index).
Conclusions

SLE patients with both anxiety and depression showed more unfavorable gut dysbiosis parameter compared to SLE patients with only anxiety or depression and SLE patients without anxiety or depression. There was positive correlation between proportion of Bacteroides and lupus disease activity and negative correlation between diversity indices and lupus disease activity.
Partitioning of REE between calcite and carbonatitic melt containing P, S, Si at 900-650 °C and 100 MPa
Authors: Chebotarev Dmitry A. , Wohlgemuth-Ueberwasser Cora 
Published: September 2021

Abstract
Many carbonatites host deposits of REE and HFSE, and fractional crystallization might be a potentially powerful mechanism controlling magma enrichment by these metals to economically significant values. At present, information about the control of fractional crystallization by partition coefficients of ore-forming elements at magmatic stage is incomplete. Here we present an experimental study of REE partitioning between carbonatite melt and calcite in the system CaCO3-Na2CO3 with varying amounts of P2O5, F, Cl, SiO2, SO3 at 650–900°C and 100 MPa using cold-seal pressure vessels and LA-ICP-MS. The presence of phosphorus in the system generally increases the distribution coefficients but its effect decreases with increasing concentration. The influence of temperature is great: at 900 − 770°C DREE ≥1, while at lower temperatures the values are below unity. Silicon also promotes the fractionation of REE into calcite, while sulfur contributes to the retention of REE in the melt. Our results imply that calcite may impose significant control upon REE fractionation at the early stages of crystallization of carbonatite magma and can be a closest proxy for monitoring the REE content in initial melt.
Functional IKK/NF-κB Signaling in Pancreatic Stellate Cells is Essential to Prevent Autoimmune Pancreatitis
Authors: Chan Lap Kwan , Tsesmelis Miltiadis , Gerstenlauer Melanie , Leithäuser Frank , Kleger Alexander , Maier Harald , Wirth Thomas 
Published: September 2021

Abstract
Pancreatic stellate cells (PSCs) are resident cells in the exocrine pancreas which contribute to pancreatic fibrogenesis and inflammation. Studies on NF-κB in pancreatitis so far focused mainly on the parenchymal and myeloid compartments. Its function in PSCs during chronic pancreatitis has not been investigated. Here we show a protective immunomodulatory function of NF-κB in PSCs. Conditional deletion of NEMO(IKKγ) in PSCs leads to spontaneous pancreatitis with elevated circulating IgM, IgG and antinuclear autoantibodies (ANA) within 18 weeks. When further challenged with experimental chronic pancreatitis, NEMOΔCol1a2 mice show an exacerbated autoimmune phenotype characterized by increased infiltration of eosinophils, B and T lymphocytes with extremely reduced latency period. These mice fail to recover even 18 weeks after the induction of pancreatitis but show sustained fibrosis and elevated eosinophil infiltration. Transcriptomic profiling shows that NEMOΔCol1a2 mice display molecular signatures resembling autoimmune pancreatitis patients. Mechanistically, we show that PSCΔNEMO cells have higher fibrogenic activities (upregulated Col1a1 and Col3a1) and produce high levels of eotaxin-2 (CCL24) which contributes to eosinophil recruitment. Corticosteroid treatment strongly attenuates the autoimmune phenotype in NEMOΔCol1a2 mice. Our findings uncover an important and unexpected immunomodulatory role specifically of NF-κB in PSCs and its deregulation may be a trigger of autoimmune pancreatitis.
HPV vaccine hesitancy among medical students in China: A multicenter survey
Authors: zhou liangru , Wang Jian , Cheng Pengxin , Li Yue , Zhang Xin , Liu Guoxiang 
Published: September 2021

Abstract
Background: In order to examine HPV information sources, vaccine hesitancy, and the association between the two variables. An online survey of HPV information sources and vaccine hesitancy was conducted among Chinese medical students. Methods: The World Health Organization (WHO) Vaccine Hesitancy 3C model was used to evaluate reasons for respondents' vaccine hesitancy. A probit model was used to investigate the association between vaccine information sources and vaccine hesitancy. Results: The reported rate of vaccine hesitancy was 62.36%. Convenience was the primary factor for vaccine hesitancy in medical students, and 37.23% used a single source to obtain vaccine information. A multivariate analysis revealed that men were 9% more likely to be hesitant about the HPV vaccine than women. Respondents without partners were 15% less hesitant than those with a partner. Respondents receiving HPV information through the Internet were 12% less likely to report vaccine hesitancy than those receiving information from other information channels. Conclusions: HPV vaccine hesitancy requires more attention. Future studies could examine whether increasing vaccination locations and dissemination of information about the safety and effectiveness of HPV vaccines as well as using Internet media would help reduce medical students’ vaccine hesitancy and expand HPV vaccine coverage. Trial registration: The study was conducted according to the guidelines of the Declaration of Helsinki,and approved by the Harbin Medical University School of Health Management & nstitutional Research Board (reference No.HMUIRB20210006, approved on 30 June 2021).
1A Nationwide Analysis of U.S. Racial/Ethnic Disparities in Emergency Department Patients with Mental Health and Substance Use Disorders
Authors: Weinreb Joanne , Gavrilova Penina , Avery Jonathan , Murphy Sean M. , Pathak Jyotishman 
Published: September 2021

Abstract
BackgroundRacial and ethnic health disparities have been linked with inequalities in access to health care and outcomes. The present study considers whether inequalities persist between racial/ethnic groups among patients with mental health or substance use disorders who visit the emergency department (ED). MethodsWe analyzed data from the National Hospital Ambulatory Medical Care Survey (NHAMCS) from 2012-2018, assessing health disparities among patients diagnosed with mental health or substance use disorders by observing whether significant differences exist in ED wait time and length of visit (LOV) for patients of different races/ethnicities. Stratified models were performed to further understand the impact of regions across the U.S., year, and triage level on the association analysis. ResultsFrom 2012-2018, non-Hispanic Black and Hispanic patients experienced significantly longer ED wait times and LOV as compared to White patients. Patients with private insurance experienced significantly shorter wait times compared to patients with self-pay, and shorter LOV than those with Medicaid/ Children’s Health Insurance Program, or Medicare. Male patients had significantly longer LOV compared to female patients. We observed year by year differences in wait times of non-Hispanic Black patients with improvement appearing between the years 2013 to 2016, while LOV remained consistently longer. We observed both regional and triage level differences, with the U.S. Northeast presenting with the most disparities. Additionally, we noted a general upward trend of SUD diagnoses. Conclusion Our analysis suggests that while there has been an overall improvement in median ED wait time through the years, non-Hispanic Black and Hispanic patients experience significantly longer ED wait time compared to non-Hispanic White patients. Additionally, non-Hispanic Black and Hispanic patients have a significantly longer ED LOV compared to non-Hispanic White patients.
Variation in waterlogging response among ecotypes of Trifolium subterraneum ssp. yanninicum and their relationships with site of origin
Authors: Enkhbat Gereltsetseg , Ryan Megan H. , Nichols Phillip G. H. , Foster Kevin J. , Inukai Yoshiaki , Erskine William 
Published: September 2021

Abstract
Background and AimsIn the annual pasture legume Trifolium subterraneum, ssp. yanninicum exhibits higher waterlogging tolerance than ssp. brachycalycinum and ssp. subterraneum. This study investigates waterlogging tolerance within ssp. yanninicum ecotypes and explores correlations with seedling phenotypic traits and site of origin eco-geographic variables.MethodsTwenty eight diverse ssp. yanninicum ecotypes collected from the Mediterranean region and four cultivars were grown in a controlled environment glasshouse. After 14 days of growth seedling traits were measured. After 21 days of growth, free-drained (control) and waterlogged treatments were imposed for 28 days. Eco-geographic variables were generated from ‘WorldClim’ using collection site locations.ResultsUnder waterlogging, shoot relative growth rate (RGR) ranged from 87–108% and root RGR ranged from 80–116% of controls. Waterlogging reduced shoot dry weight (DW) in four of 32 genotypes, while root DW was reduced in 13 genotypes. Leaf size was maintained, or even increased, under waterlogging in 31 genotypes. However, petiole length was more affected by waterlogging and has value as a waterlogging tolerance indicator. Waterlogging tolerance was not significantly correlated with seedling DW, flowering time or precipitation at the site of origin, while shoot growth under waterlogging had a positive correlation with summer temperatures at origin.ConclusionsGenotypes of ssp. yanninicum tolerated transient waterlogging and greater tolerance was observed among ecotypes, rather than cultivars. An easy-to-measure indicator of tolerance was found in petiole length reduction. This study highlights untapped genotypic variability for breeders to improve the productivity and persistence of ssp. yanninicum under waterlogging.
Use of facemasks during the covid-19 pandemic in southeastern Nigeria: an observational study
Authors: Chukwuocha Uchechukwu Madukaku , Ogboeze Joshua Chisom , Bosede Ayoola Oluwaseun , Oduenyi Lilian Anulike , Chukwujekwu Amarachukwu Blessing , Okoye Sandra Chidubem , Madubuike Chigozirim Favour , Agu Amarachi Regina 
Published: September 2021

Abstract
Background

The use of facemask for the control of the spread of COVID-19 virus among the population has been recommended by the health authorities. This observational study was carried out to assess the use of facemask in Southeastern Nigeria.
Methods

Using a two-stage sampling technique, a total of 3100 participants were observed from both rural and urban settings. Frequency distribution tables were used to categories and describe the observed variables, Chi-square (X2) test was used to check for the association between categorical variables.
Results

Among the observed participants, 46.4% made use of facemask. The most common facemask used was cloth mask (28.6%). About 16.0% of the participants correctly used their facemasks. The highest usage was observed in the urban location (49.2%). A statistically significant association was found between facemask usage and study location (p < 0.001), also between the appropriateness of usage and age category (p < 0.001).
Conclusions

The observed rate of mask usage may not be able to protect the population against the spread of COVID-19, therefore adequate sensitization on the need for proper use of face masks by the public should be prioritized.
Chemical durability of Gd2Zr2O7 transparent ceramics under different pH conditions
Authors: Liu Kui , Zhang Kuibao , Li Weiwei , Yang Qian , Zhang Haibin 
Published: September 2021

Abstract
To study the influence of acidic and alkaline environments on the leaching behavior of Gd2Zr2O7 immobilization matrix in deep geological landfills, and to directly reflect the change of leaching behavior on its appearance, the chemical durability of Gd2Zr2O7 transparent ceramics was investigated in different pH environments in this study. The leaching results demonstrate that the normalized leaching rates in acidic environments are higher than those in alkaline and neutral environments. The transmittance of the leached transparent ceramics in the visible range decreases slightly at pH ≤ 9.0, and the macroscopic color of all samples fades after leaching. The leaching behavior leads to lattice contraction and increment of surface roughness, but it exhibits no significant effect on the surface elemental distribution. In conclusion, Gd2Zr2O7 transparent ceramics show excellent chemical durability in a weak alkaline environment, and the leaching behavior causes the discoloration of Gd2Zr2O7 transparent ceramics.