bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-498 targets UBE2T to Inhibit Proliferation and Metastasis in Malignant Melanoma
Authors: Dang Ningning , Cao Wen , Li Pin , Huang Shuhong 
Published: June 2021

Abstract
BackgroundMalignant melanoma (MM) is a common malignant tumor and also one of the fastest growing malignancies. Considering that UBE2T could be a direct target of miR-498, we explore the ability of miR-498 to regulate MM cell progression by targeting UBE2T.MethodsWe detected the expression of miR-498 by quantitative real-time PCR assay. The cell proliferation, invasion and migration in MM cells were measured by cell counting kit-8, clone formation and transwell assays. Flow cytometry assay was used to detect the percentage of apoptotic cells. We explored the correction of miR-498 and UBE2T by dual-luciferase assays.ResultsDuring the study, we found that the overexpression of miR-498 inhibited proliferation, invasion, migration and induced cell apoptosis of M14 and A375 cells. In addition, the expression of epithelial-mesenchymal transition (EMT)-related factors was altered by overexpression of miR-498. Furthermore, UBE2T was differentially expressed in MM and normal tissues and high expression of UBE2T was strongly correlated with poor overall survival in patients with MM. We highlighted that overexpression of UBE2T reversed the inhibitory effects of miR-498 on MM cell progression.ConclusionsAltogether, our findings demonstrated that miR-498 significantly inhibited cell proliferation, invasion, migration and induced apoptosis, whilst confirming that miR-498 regulated MM cell progression by targeting UBE2T.
Critical Issue On The Extinction and Inattention Subtest of NIHSS Scale. An Analysis on Post-Acute Stroke Patients Attending Inpatient Rehabilitation
Authors: Basagni Benedetta , Hakiki Bahia , Campagnini Silvia , Salvadori Emilia , Grippo Antonello , Paperini Anita , Castagnoli Chiara , Hochleitner Ines , Politi Angela Maria , Gemignani Paola , Mosca Irene Eleonora , Franceschini Azzurra , Bonotti Enrico Bacci , Sodero Alessandro , Mannini Andrea , Pellicciari Leonardo , Poggesi Anna , Macchi Claudio , Carrozza Maria Chiara , Cecchi Francesca 
Published: June 2021

Abstract
Objectives
This study aims to evaluate the diagnostic performance of NIHSS extinction and inattention item, compared to the results of the Oxford Cognitive Screen (OCS) heart subtest. Additionally, the possible role of the NIHSS visual field subtest on the NIHSS extinction and inattention subtest performance is explored and discussed.
Methods
We analysed scores on NIHSS extinction and inattention subtest, NIHSS visual field subtest, and OCS heart subtest on a sample of 118 post-stroke patients.
Results
Compared to OCS heart subtest, the results on NIHSS extinction and inattention subtest showed an accuracy of 77.1% and a moderate agreement level (Cohen’s kappa = 0.455). Furthermore, a decrease in NIHSS accuracy detecting neglect (55.6%) was observed in patients with pathological scores in NIHSS visual field item.
Conclusions
Extreme caution is recommended for the diagnostic performance of extinction and inattention item of NIHSS. Signs of neglect may not be detected by NIHSS, and may be confused with visual field impairment.
Trial registration:
This study refers to an observational study protocol submitted to ClinicalTrials.gov with identifier: NCT03968627 (URL: https://www.clinicaltrials.gov/ct2/show/NCT03968627?term=Cecchi&cond=Stroke&draw=2&rank =2). The name of the registry is “Development of a National Protocol for Stroke Rehabilitation in a
A High Urinary Urobilinogen / Serum Total Bilirubin Ratio Reported in Abdominal Pain Patients Can Indicate Acute Hepatic Porphyria
Authors: Song Chengyuan , Sang Shaowei , Liu Yuan 
Published: June 2021

Abstract
Background: Due to its variable symptoms and nonspecific laboratory test results during routine examinations, acute hepatic porphyria (AHP) has always been a diagnostic dilemma for physicians. Misdiagnoses, missed diagnoses, and inappropriate treatments are very common. Correct diagnosis mainly depends on the detection of a high urinary porphobilinogen (PBG) level, which is not a routine test performed in the clinic and highly relies on the physician’s awareness of AHP. Therefore, identifying a more convenient indicator for use during routine examinations is required to improve the diagnosis of AHP.Results: In the present study, we retrospectively analyzed laboratory examinations in 12 AHP patients and 100 patients with abdominal pain of other causes as the control groups between 2015 and 2021. Compared with the control groups, AHP patients showed a significantly higher urinary urobilinogen level during the urinalysis (P < 0.05). However, we showed that the higher urobilinogen level was caused by a false-positive result due to a higher level of urine PBG in the AHP patients. Hence, we used serum total bilirubin, an upstream substance of urinary urobilinogen synthesis, for calibration. A remarkable increase in the urinary urobilinogen/serum total bilirubin ratio was observed in AHP patients when compared to the control groups. The area under the ROC curve of this ratio for AHP was 1.000 (95% confidence interval, 1.000–1.000, P < 0.01). A cutoff value of 3.22 for the urinary urobilinogen/serum total bilirubin ratio yielded a sensitivity of 100% and a specificity of 100% to distinguish AHP patients from the controls.Conclusion: A reported high urinary urobilinogen level that was adjusted by the serum total bilirubin level (urinary urobilinogen/serum total bilirubin ratio) could be used as a sensitive and specific screening marker for AHP in patients with abdominal pain.
Regulatory Network of miRNA, lncRNA, Transcription Factor and Target Immune Response Genes in Bovine Mastitis
Authors: Morenikeji Olanrewaju , Tucker Ashley , Salazar Nicole , Ayoola Adeola , Memili Erdogan , Thomas Bolaji 
Published: June 2021

Abstract
Pre- and post-transcriptional modifications of gene expression are emerging as foci of disease studies, with some studies revealing the importance of non-coding transcripts, like long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). We hypothesize that TFs, lncRNAs and miRNAs can modulate the immune response in bovine mastitis and potentially serve as disease biomarkers and/or drug targets. With computational analyses, we identified candidate genes potentially regulated by miRNAs and lncRNAs base pair complementation and thermodynamic stability of binding regions. Remarkably, we found six miRNAs, two being bta-miR-223 and bta-miR-24-3p, to bind to several targets. NONBTAT027932.1 and XR_003029725.1, were identified to target several genes. Functional and pathway analyses revealed lipopolysaccharide-mediated signaling pathway, regulation of chemokine (C-X-C motif) ligand 2 production and regulation of IL-23 production among others. The overarching interactome deserves further in vitro/in vivo explication for specific molecular regulatory mechanisms during bovine mastitis immune response and could lay the foundation for development of disease markers and therapeutic intervention.
Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level
Authors: Fan Rong , Deng Yanxiang , Bartosovic Marek , Ma Sai , Zhang Di , Liu Yang , Qin Xiaoyu , Su Graham , Xu Mina , Halene Stephanie , Craft Joseph , Castelo-Branco Goncalo 
Published: June 2021

Abstract
Cellular function in tissue is dependent upon the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping, but it remains elusive to capture spatial epigenetic information of tissue at cellular level and genome scale. Here we report on spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissue section via next-generation sequencing by combining in situ Tn5 transposition chemistry and microfluidic deterministic barcoding. Spatial chromatin accessibility profiling of mouse embryos delineated tissue region-specific epigenetic landscapes and identified gene regulators implicated in the central nerve system development. Mapping the accessible genome in human tonsil tissue with 20μm pixel size revealed spatially distinct organization of immune cell types and states in lymphoid follicles and extrafollicular zones. This technology takes spatial biology to a new realm by enabling spatially resolved epigenomics to improve our understanding of cell identity, state, and fate decision in relation to epigenetic underpinnings in development and disease.
Usefulness of the MADIT-ICD Benefit Score in a S-ICD Patient Cohort
Authors: Willy Kevin , Köbe Julia , Reinke Florian , Rath Benjamin , Ellermann Christian , Wolfes Julian , Lange Philipp S , Eckardt Lars , Frommeyer Gerrit 
Published: June 2021

Abstract
Background: The subcutaneous ICD (S-ICD) is used in an increasing number of patients for primary and secondary prevention of sudden cardiac death. Decision-making in primary prevention is not always trivial and many clinical scenarios are not reflected in current ICD guidelines. To help evaluating the patient’s individual risk, a new score trying to predict the benefit of an ICD implantation for primary prevention, the MADIT-ICD benefit score, which tries to predict occurrence of ventricular arrhythmias and non-arrhythmic death, has been proposed. We therefore aimed at examining its usefulness in a large single centre register of S-ICD patients Methods and results: All S-ICD patients with a primary preventive indication for ICD implantation from our large single centre database were included in the analysis (n=173). During a follow-up of 1227±978 days 27 patients developed sustained ventricular arrhythmias, while 6 patients died for non-arrhythmic reasons. Occurrence of ventricular arrhythmias could not sufficiently be predicted by the MADIT-ICD VT/VF score (p=0.3) in patients with (n=142, p=0.19) as well as patients without structural heart disease (n=31, p=0.88). However, there was a significant correlation for patients with ischemic cardiomyopathy (ICM) (n=29, p=0.04). Only one parameter (non-sustained ventricular tachycardia) was significantly associated with sustained ventricular arrhythmias (p=0.02). Of note, non-arrhythmic death could effectively be predicted by the proposed non-arrhythmic mortality score as part of the benefit score (p=0.001, r=0.3) also mainly driven by ICM patients. Age, diabetes mellitus, and a BMI <23 kg/m2 were key predictors of non-arrhythmic death implemented in the score.Conclusion: The MADIT-ICD benefit score adds a new option to evaluate expected benefit of ICD implantation for primary prevention. However, in our S-ICD cohort, the only group in which the score worked properly for prediction of sudden and non-sudden death were ICM patients, so that a larger validation in more heterogeneous cohorts in mandatory to claim general validity for this score in risk stratification for primary preventive ICD implantation.
Percutaneous Endoscopic Lumbar Discectomy for Treating Cauda Equina Syndrome Due To Lumbar Disc Herniation
Authors: He Hai-Chao , LV Xiao-qiang , Zhang Yong-Jin 
Published: June 2021

Abstract
Background

In recent decades, endoscopic techniques to treat lumbar disc herniation (LDH) have gained popularity in clinical practice. However, there is little literature on the use of percutaneous endoscopic lumbar discectomy (PELD) to treat cauda equina syndrome (CES) due to LDH. This study aims to evaluate the feasibility and clinical efficacy of PELD for treating CES caused by disc herniation, and as well as to report some technical strategies.
Methods

Between October 2012 and April 2018, 15 patients with CES caused by LDH at the early and intermediate stages of Shi’s classification were selected as the subjects of study, and underwent PELD. All patients were followed up for at least two years. The patients’ back pain and leg pain were evaluated using visual analogue scale (VAS) scores and the Oswestry Disability Index (ODI). Patient satisfaction was evaluated using the MacNab outcome scale. Clinical outcomes were measured preoperatively and at 3 days, 3 months, 6 months and the last follow-up.
Results

The VAS score for back pain, leg pain and ODI score significantly decreased from preoperatively scores of 6.67 ± 1.05, 7.13 ± 1.19 and 62.0 ± 6.85 respectively, to postoperatively cores of 1.80 ± 0.41, 1.47 ± 0.52 and 12.93 ± 1.03 at the last follow-up postoperatively. These postoperative scores were all significantly different compared with preoperative scores (P < 0.01). According to the modified MacNab outcome scale, 86.67% of these patients had excellent and good outcomes at the final follow-up. Complications included one patient with cerebrospinal fluid leakage and one patient who developed recurrent herniation; the latter patient finally achieved satisfactory results after reoperation.
Conclusion

PELD could be used as an alternative surgical method for the treatment of CES due to LDH in properly selected cases and appropriate patient selection. However, the operator should pay attention to foraminoplasty to enlarge the working space.
Aldosterone and Renin Concentration Abnormally Elevated in a Cohort of Normotensive Pregnant Women
Authors: Pasten Valentina , TAPIA-CASTILLO ALEJANDRA , Fardella Carlos E. , Leiva Andrea , Carvajal Cristian A. 
Published: June 2021

Abstract
During pregnancy the renin-angiotensin-aldosterone system (RAAS) undergo major changes to preserve normal blood pressure (BP), placenta blood flow and ensure a good pregnancy outcome. Abnormal aldosterone-renin metabolism is a risk factor for arterial hypertension and cardiovascular risk, but its association to pathological conditions in pregnancy remains unknown. Hence, clinical and subclinical novel biomarkers associated to these pathological conditions are encouraged to be identified. Aim: To study a cohort of normotensive pregnant women according to their serum aldosterone and plasma renin levels and assay their small extracellular vesicles (sEV) and a specific protein-cargo (LCN2, AT1R). Methods: A cohort of 54 normotensive pregnant women at term gestation. We determined the BP, serum aldosterone and plasma renin concentration. In a subgroup, we isolated their plasma sEVs and semi-quantify two EV-proteins (AT1R, LCN2). Results: We set a normal range of aldosterone and renin based in the interquartile range. We identified 5/54(9%) pregnant women with elevated aldosterone and low renin levels, and 5/54(9%) other pregnant women with low aldosterone and elevated renin levels. No differences were found in sEV-LCN2 nor sEV-AT1R. Conclusion: We found 18% normotensive pregnant women having either high aldosterone or high renin levels, suggesting a subclinical status similar to a primary aldosteronism or a hyperreninemia. Both would evolve to pathological conditions in presence a second challenge affecting the maternal vascular physiology or the BP. sEVs and its specific cargo are encouraged to be further studied in order to clarify its role as potential biomarkers of RAAS alterations in pregnant women.
Identifying the Chemical Profile and Pharmacological Mechanism of Xinbao Pill against Myocardial Ischemia-Reperfusion Injury Using Ultra-Fast Liquid Chromatography-Quadrupole-Time-Of-Flight Tandem Mass Spectrometry Coupled with Network Pharmacology-Based Investigation
Authors: Yang Ying , Chen Ting , Liu Jiaming , Chen Sixuan , Cai Rongqing , Wu Liqiong , Hu Jiexiong , Lin Qiongying , Qi Xiaoxiao , Liu Zhongqiu , Cheng Yuanyuan 
Published: June 2021

Abstract
Background: Xinbao Pill (Xin-Bao-Wan, XBW) is a traditional Chinese herbal formula, which is widely used in clinical treatment for cardiovascular diseases; however, the therapeutic effect of XBW on myocardial ischemia-reperfusion injury (MI/RI) is unclear. In this study, we evaluated the cardioprotective effect and molecular mechanism of XBW against MI/RI.Methods: A phytochemistry-based network pharmacology analysis was used to uncover the mechanism of XBW against MI/RI. Firstly, ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method was used to identify chemicals in XBW. MI/RI-related targets of XBW were predicted using TargetNet database, OMIC database and reported literatures. GO and KEGG pathway enrichment analyses were performed using String database. Left anterior descending artery (LAD) ligation-induced MI/RI rat model and oxygen glucose deprivation-reperfusion (OGD/R)-induced H9c2 cell model were used to evaluate the cardioprotective effect and potential mechanism of XBW.Results: 37 chemicals were identified in XBW by using UHPLC-QTOF-MS; 50 MI/RI-related targets of XBW were predicted using TargetNet database, OMIC database and reported literatures. In vivo study, XBW (180 mg/kg) significantly reduced myocardial infarct size and creatine kinase MB (CK-MB) level induced by MI/RI in rat model; in vitro study, XBW protected H9c2 cells against OGD/R injury in a dose-dependent manner. GO and KEGG pathway enrichment analyses showed that the cardioprotective effect of XBW was associated with 5 significant pathways including autophagy, apoptosis, HIF-1 signaling pathway, PI3K-Akt signaling pathway, FoxO signaling pathway. Experimental investigation also verified that XBW could suppress apoptosis, autophagy and endoplasmic reticulum (ER) stress.Conclusion: XBW showed therapeutic effects against MI/RI mainly via attenuating apoptosis though suppressing excessive autophagy and ER stress.
A Retrospective Study on Incidence, Diagnosis, and Clinical Outcome of Gastric-type Endocervical Adenocarcinoma in a Single Institution
Authors: Radomska Anna , Lee Daniel , Neufeld Heather , Korte Nancy , Torlakovic Emina , Agrawal Anita , Chibbar Rajni 
Published: June 2021

Abstract
Background: Gastric-type endocervical adenocarcinoma is rare but the most common subtype of cervical adenocarcinoma not associated with human papillomavirus. It is more aggressive with a shorter five-year survival rate compared to human papillomavirus-associated usual type endocervical adenocarcinoma. The objectives of our study were to determine the incidence and clinical-pathological characteristics of Gastric-type endocervical adenocarcinoma in a single institution. Methods: Twenty four cases of invasive cervical adenocarcinoma were identified between January 2000 and December 2015, from the Saskatoon Health Region pathology database using International Endocervical Adenocarcinoma Criteria and Classification to retrospectively classify endocervical adenocarcinoma. Immunohistochemistry was performed with antibodies for Gastric mucin-6 (MUC-6), p16INK4a, cyclin-dependent kinase inhibitor 2A (p16), p53 protein (p53), estrogen and progesterone receptors. Clinical and pathological data was retrieved from pathology reports and charts. Statistical analysis was performed using Mann-Whitney U test and Chi-Square test.Results: Using the International Endocervical Adenocarcinoma Criteria and Classification criteria, 19 cases (79.2%) were classified as human papillomavirus-associated usual type endocervical adenocarcinoma, and five cases (20.8%) as Gastric-type endocervical adenocarcinoma. In our study 40% of Gastric-type endocervical adenocarcinoma cases presented at stage III compared to none of the usual type endocervical carcinoma cases. All the Gastric-type endocervical adenocarcinoma cases were positive for MUC-6, and negative for p16. 60% Gastric-type endocervical adenocarcinoma cases demonstrated mutant type p53 staining. In contrast, 84.2% of human papillomavirus-associated usual type endocervical adenocarcinoma cases showed block like nuclear and cytoplasmic positivity with p16 antibodies. The Gastric-type endocervical adenocarcinoma group had significantly shorter median survival time than human papillomavirus-associated usual type endocervical adenocarcinoma group, Gastric-type endocervical adenocarcinoma is 22 months compared to human papillomavirus-associated usual type endocervical adenocarcinoma at 118 months (p = 0.043). Conclusions: In this study, Gastric-type endocervical adenocarcinoma accounted for 20.8% of all cervical adenocarcinoma with higher stage at presentation and shorter overall survival. Histomorphology and immunohistochemistry for MUC-6 and p16 could differentiate between Gastric-type endocervical adenocarcinoma and human papillomavirus-associated usual type endocervical adenocarcinoma.
Recurrent Pancreatic Follicular Dendritic Cell Sarcoma: A Case Report and Literature Review
Authors: Li Jia , Zhou Wenyang , Lv Ying , Hu Rong 
Published: June 2021

Abstract
Background: Follicular Dendritic Cell Sarcoma (FDCS) is a rare malignant tumor originated from follicular dendritic cells. Presently radical resection is the standard treatment; however there is no clear optimal chemotherapy for unresectable lesions. We presented the clinical data, pathological features, imaging features, diagnosis and treatment of a very rare patient with recurrence and metastasis after radical resection of pancreatic FDCS. Case presentation: A 64-year-old male patient was delivered to hospital due to intermittent chest tightness and pain. A pancreatic mass was found after thorough physical examination. Enhanced MRI showed a round solid mass in the head and neck of pancreas. The patient underwent radical resection. The pathological diagnosis was FDCS. CHP regimen was used for consolidation chemotherapy for 6 cycles. Two years later, due to a large amount of effusion, the patient was confirmed to have relapse and extensive abdominal metastasis. Both CHOP regimen and bendamustine were ineffective.Conclusions: FDCS of pancreas is very rare, and its imaging findings are not specific. If the disease relapses or metastases, though applies a variety of chemotherapy regimens, it is difficult to obtain satisfactory curative effect, and long-term prognosis is pessimistic. This is the first reported case that bendamustine was ineffective in the treatment of FDCS.
Metastatic Pattern of Truncal and Extremity Leiomyosarcoma: Retrospective Analysis of Predictors, Outcomes, and Detection
Authors: Tigchelaar Seth Stravers , Frey Christopher , Segovia Nicole A. , Mohler David G. , Steffner Robert J. , Avedian Raffi S. 
Published: June 2021

Abstract
BackgroundLeiomyosarcomas (LMS) are a heterogenous group of malignant mesenchymal neoplasms with smooth muscle origin and are classified as either non-uterine (NULMS) or uterine (ULMS). Metastatic pattern, prognostic factors, and ideal staging/surveillance studies for truncal and extremity LMS have not been defined.MethodsA retrospective analysis of patients diagnosed with histopathology-confirmed truncal or extremity LMS between 2009-2019 was conducted. Data collected included demographics, tumor characteristics, staging, surveillance, and survival endpoints. Primary site was defined as either 1. Extremity, 2. Flank/Pelvis, or 3. Chest wall/Spine.ResultsWe identified 73 patients, 23.3% of which had metastatic LMS at primary diagnosis, while 68.5% developed metastatic disease at any point. The mean metastatic-free survival from primary diagnosis of localized LMS was 3.0±2.8 years. Analysis of prognostic factors revealed that greater age (≥50 years) at initial diagnosis (OR=3.74, p=0.0003) and higher tumor grades II (OR=16, p=0.019) and III (OR=13, p=0.025) were significantly associated with metastases.ConclusionsTruncal and peripheral extremity LMS is an aggressive tumor with high metastatic potential. While there is a significant risk of metastases to lungs, extra-pulmonary tumors are relatively frequent. Older patients and patients with higher-grade (II/III) tumors carry an increased risk of developing metastatic disease.