bluuebunny/crossref_metadata_2025_split huggingface dataset:
The RNA-binding protein LUC7L2 mediates MITA/STING intron retention to negatively regulate innate antiviral response
Authors: Li Chen , Feng Lu , Luo Wei-Wei , Lei Cao-Qi , Li Mi , Shu Hong-Bing 
Published: June 2021

AbstractMITA (also known as STING) is an ER-located adaptor protein, which mediates DNA-triggered innate immune response and is critically involved in autoimmune diseases and tumorigenesis. MITA is regulated by post-translational modifications, but how post-transcriptional mechanisms are involved in the regulation of MITA is still largely unknown. Here, we identified the RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. LUC7L2-deficient mice exhibited resistance to lethal herpes simplex virus 1 (HSV-1) infection and reduced HSV-1 loads in the brain. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing and promoted its nonsense-mediated decay, leading to its downregulation at protein level. LUC7L2-deficient cells had markedly increased MITA level, leading to heightened innate antiviral response. Finally, LUC7L2 was induced following HSV-1 infection. Our findings reveal a feedback negative post-transcriptional regulatory mechanism for regulation of MITA-mediated innate immune response to viral and aberrant cellular DNA.
The immune cell landscape of peripheral blood mononuclear cells from PNS patients
Authors: Ye Qing , Zhou Chao , Li Sisi , Wang Jingjing , Liu Fei , Liu Zhixia , Mao Jianhua , Fu Haidong 
Published: June 2021

AbstractExisting research suggests that the human immune system and immune cells are involved in the pathogenesis of nephrotic syndrome, but there is still a lack of direct evidence. This study tried to analyze the profiling of immune cells in the peripheral blood of steroid-sensitive nephrotic syndrome (SSNS) patients and steroid-resistant nephrotic syndrome (SRNS) patients before and after standard steroid treatment to clarify the immunological mechanism of nephrotic syndrome patients. The number and proportion of CD4 + T cells in patients with nephrotic syndrome remained unchanged. However, there is an imbalance of Th1 and Th2 and an excessive increase of Th17 cells. The number of CD8 + T cells and the number of effector CD8 + T cells in them increased significantly, but only in SSNS, the number of activated CD8 + T cells increased, and the number of activated Tregcells decreased significantly. Nephrotic syndrome patients also have B cell disorder, and it is more prominent in SSNS patients. Compared with the normal control, only the number of B cells and plasmablast in SSNS patients increased significantly (Z = − 2.20,P = 0.028). This study also observed that transitional B cells decreased in both SSNS and SRNS patients, but SSNS patients' decrease was lower than in SRNS patients. Compared with normal controls, monocytes in patients with nephrotic syndrome decreased significantly. The main reason was that Non-classical Monocyte decreased, while Classical Monocyte increased slightly. The total number of NK cells did not change, but the internal cell subgroups' composition occurred. Changes, realized as CD56hi NK cells increased, CD56low NK cells decreased; and the above trend is more evident in SSNS patients. Patients with nephrotic syndrome have immune disorders, including T cells, B cells, Monocytes, and NK cells. It can be confirmed that immune factors are involved in the pathogenesis of the nephrotic syndrome.
The effect of the flavonol rutin on serum and liver iron content in a genetic mouse model of iron overload
Authors: Hawula Zachary J. , Secondes Eriza S. , Wallace Daniel F. , Rishi Gautam , Subramaniam V. Nathan 
Published: June 2021

Abstract
The flavonol rutin has been shown to possess antioxidant and iron chelating properties in vitro and in vivo. These dual properties are beneficial as therapeutic options to reduce iron accumulation and the generation of reactive oxygen species (ROS) resultant from excess free iron. The effect of rutin on iron metabolism has been limited to studies performed in wildtype mice either injected or fed high-iron diets. The effect of rutin on iron overload caused by genetic dysregulation of iron homoeostasis has not yet been investigated. In the present study we examined the effect of rutin treatment on tissue iron loading in a genetic mouse model of iron overload, which mirrors the iron loading associated with Type 3 hereditary haemochromatosis patients who have a defect in Transferrin Receptor 2 (TFR2). Male TFR2 knockout (KO) mice were administered rutin via oral gavage for 21 continuous days. Following treatment, iron levels in serum, liver, duodenum and spleen were assessed. In addition, hepatic ferritin protein levels were determined by Western blotting, and expression of iron homoeostasis genes by quantitative real-time PCR. Rutin treatment resulted in a significant reduction in hepatic ferritin protein expression and serum transferrin saturation. In addition, trends towards decreased iron levels in the liver and serum, and increased serum unsaturated iron binding capacity were observed. This is the first study to explore the utility of rutin as a potential iron chelator and therapeutic in an animal model of genetic iron overload.
A Novel Protocol Designed to Treat Spastic Dysarthria Secondary to Traumatic Brain Injury: A Case Study
Authors: Galek Kristine , Bice Ed M. , Allen Katie 
Published: June 2021

IntroductionSpastic dysarthria is a motor speech disorder produced by bilateral damage to the activation pathways of the central nervous system. Its speech characteristics reflect the effects of hypertonicity and weakness of the bulbar musculature in a way that slows movement and reduces range of motion and force. Perceptually, speech has a high-pitched, strained, hypernasal vocal quality with decreased intelligibility.PurposeThe purpose is to present a case illustration describing the use of a novel treatment protocol to improve speech intelligibility in the presence of spastic dysarthria.MethodAn underlying framework, including principles of exercise, neuroplasticity, and motor learning with adjunctive biofeedback, is described. The protocol consisted of four sessions per week for 4 weeks with daily homework.ResultsThe participant exhibited improvements in intelligibility, patient satisfaction, lingual, and jaw range of motion, nasality, and tongue strength.ConclusionThe use of a novel protocol using biofeedback and incorporating principles of exercise science, neuroplasticity, and motor learning for the treatment of spastic dysarthria demonstrated positive outcomes.
Effects of Repeated Exposure to Synthetic and Digitized Natural Speech by Individuals With Aphasia
Authors: Wollersheim Madeline , Brown Jessica A. , Hux Karen , Knollman-Porter Kelly , Wallace Sarah E. 
Published: June 2021

Purpose
Technological supports provide multimodal presentation of text, which may increase understandability of written materials by people with aphasia (PWA). Extant literature reveals that digitized natural speech and synthetic computer-generated speech within these supports are comprehensible by PWA at discrete time points; however, understandability following repeated exposure is unexplored. This pilot study evaluated understanding of, and preference regarding, synthetic and digitized natural speech during and following repeated exposures by PWA.

Method
This multiple–case-study project included four adults with aphasia. Participants independently completed various listening tasks in a single digitized natural speech condition and one of two synthetic speech conditions across a 2-week period. Participants completed sessions to evaluate maintenance effects and generalization to novel stimuli and an untrained synthetic voice condition 1 week and 1 month following daily practice.

Results
Study participants demonstrated understandability evident by at- or near-ceiling performance during baseline, practice, and follow-up sessions in the digitized natural condition and one synthetic speech condition. Individuals with mild aphasia achieved relatively consistent performance during independent practice, whereas performance of individuals with moderate aphasia fluctuated in both conditions. All four participants demonstrated variable understanding during generalization and maintenance tasks at follow-up time points. Participants most frequently indicated preference for digitized natural speech over either synthetic speech condition.

Conclusions
Findings hold important clinical implications when considering feasibility of utilizing these speech forms as compensatory strategies for PWA. Further research is needed to more fully understand the effects of repeated exposure to synthetic speech on comprehension among PWA.
A compact broadband ESD protection circuit using multi‐layer helical inductor
Authors: Zou Wang‐Hui , Tang Peng , Zhu Zhi‐Hua 
Published: June 2021

AbstractHere, a broadband electrostatic discharge (ESD) protection circuit using area‐efficient multi‐layer helical inductors is presented. The proposed concept was verified in a 0.18 μm 1P6M CMOS process, and the circuit area is only 54 × 63 μm2. The measurement results show that a bandwidth of around 30 GHz is achieved, and the impedance matching is kept under –20 dB up to 40 GHz. The measured TLP and VF‐TLP currents reach 2.19 and 5.80 A, respectively, which indicates a good ESD robustness.
A parallel multi‐block alternating direction method of multipliers for tensor completion
Authors: Zhu Hu , Wang Zhongyang , Yan Taiyu , Yu Yu‐Feng , Deng Lizhen , Bao Bing‐Kun 
Published: June 2021

AbstractThis paper proposes an algorithm for the tensor completion problem of estimating multi‐linear data under the limitation of observation rate. Many tensor completion methods are based on nuclear norm minimization, they may fail to achieve the global solution for solving nuclear norm minimization in tensor completion problem with high missing ratio. To tackle this issue, an adaptive tensor completion method based on parallel multi‐block alternating direction method of multipliers (ADMM) algorithm is proposed, it can derive the model from the initial estimate and compute the next estimate from the current solution. The parallel multi‐block ADMM with global convergence is adopted to solve the dual problem, which greatly improves the processing power and reliability of the algorithm.
An improved hysteresis current control scheme during grid voltage zero‐crossing for grid‐connected three‐level inverters
Authors: Wang Haifeng , Wu Xinzhen , Zheng Xiaoqin , Yuan Xibo 
Published: June 2021

AbstractFor three‐level inverters, hysteresis current control (HCC) has been widely used in applications such as active power filters due to its fast‐dynamic response, and it does not need to control each frequency separately with multiple controllers, which makes it simpler. However, the large fluctuation range of switching frequency is a serious disadvantage of hysteresis current control. Especially, the switching frequency decreases significantly during grid voltage zero‐crossing. This frequency fluctuation will cause low‐frequency harmonics. The purpose of this paper is to propose a sampling compensation scheme, which can solve the problem of switching frequency fluctuation near the zero‐crossing of grid voltage. Based on the variable hysteresis band strategy, the virtual sampling and switching time prediction algorithm is designed, which does not need to increase the sampling frequency or add additional controller. Therefore, nearly fixed switching frequency can be achieved while maintaining the control performance. Both simulation and experimental tests have verified the efficacy of the proposed scheme.
Tracing the magnetic field morphology of the LDN 1172/1174 cloud complex
Authors: Saha Piyali , Gopinathan Maheswar , Sharma Ekta , Won Lee Chang , Ghosh Tuhin , Kim Shinyoung 
Published: June 2021

Context. The LDN 1172/1174 cloud complex in the Cepheus Flare region presents a hub-filament structure with the reflection nebula, NGC 7023, illuminated by a Herbig Be star, HD 200775, which consists of the hub with a ~5 pc long narrow filament attached to it. Formation of a sparse cluster of low- and intermediate-mass stars is presently taking place in the hub.
Aims. The aim of this work is to map the magnetic field geometry of LDN 1172/1174 to understand the role played by the field lines in the formation of the molecular cloud.
Methods. Unpolarized background stars can be used to measure dichroically polarized light produced by the magnetically aligned grains present in molecular clouds. As these dust grains get aligned with their minor axes parallel to the ambient magnetic field, the polarization measurements can provide the plane-of-sky component of the magnetic field. We made R-band polarization measurements of 249 stars projected on the entire LDN 1172/1174 cloud complex to map the geometry of the magnetic field of this region.
Results. The magnetic field geometry constructed from our R-band polarization measurements is found to be parallel to the elongated structure inferred from the column density distribution of the cloud produced using the Herschel images. Our R-band polarization measurements are found to be in good agreement with those obtained from Planck. There is evidence of a possible distortion of the magnetic fields toward the northwestern part of the cloud by HD 200775. The magnetic field strength is estimated as ~30 μG. The estimated star formation rate (SFR)/mass of 2.0 ± 1.3% Myr−1 and 0.4 ± 0.3% Myr−1 for LDN 1172/1174 and the neighboring cloud complex, LDN 1147/1158, respectively, are found to be consistent with the mean SFR/mass found for the clouds with magnetic field orientations parallel and perpendicular to their elongated structures, respectively. These results support earlier findings that the clouds with magnetic field lines parallel to their long axes seem to have higher SFRs compared to those with the magnetic field orientation perpendicular to the cloud elongation.
White technical variety of Platovsky grapes for quality ecological winemaking
Authors: Maistrenko Alexander , Maistrenko Lyudmila , Duran Nadezhda , Matveeva Natalia 
Published: June 2021

Аbstract.
The agrobiological characteristic of Platovsky grape varietyhas been given. The variety was bred in ARRIV&W - a branch of the FSBSI FRARC as a result of crossbreeding of varieties Zala dyondye (Villar Blank × Csaba dyondye )× Podarok Magarach (Rkatsiteli × Magarach 2-57-72). The variety was included in the State Register of Breeding Achievements for Use in 2003. The technical purpose of use, very early maturation, the duration of the production period is on average 110 days with the sum of active temperatures of 2499 °C. The variety is character-ized by high winter hardiness and frost resistance. At the sum of negative temperatures 586 °C the opening of the buds was 86%, the fruit shoots - from 70 to 97. m The harvest of 1 bush is on average for 11 years 5,7 kg with the planting scheme of 3 × 1,5. Platovsky is distinguished by a good sugar-accumulation (up to 300 g/dm3) with moderate acidity and used for high quality dry and liquor wines. Resistance to mildew and oidium is very high, which allows to cultivate the variety with minimal protection from fungal diseases and use grapes for the production of bio wine.
Information technologies and resources in the daily professional activities
Authors: Volkov Yuriy , Chikarova Galina 
Published: June 2021

Based on applied sociological research results (data collection technique -in-depth interview), the main directions of using information technologies and resources by young teachers in a big Russian city are examined.
The article states that the digital transformation of the educational environment influences everyday work of young teachers.
The positive and negative instances of the digital transformation of the educational environment are highlighted. The studies have defined that the effective use of information technologies and resources is capable of minimizing some difficulties and risks faced by modern teachers in their daily professional activities. The studies have indicated new courses for the introduction of information technologies into the daily practice of the professional activity of young teachers. After conducting empirical verification of the influence of the digital transformation of the Russian society and the education system on the formation of the professional identity of young teachers in a big Russian city the authors have concluded that information technologies and resources are a constitutive factor in building professional identity of young teachers in a big Russian city.
Maintenance treatment in relapsed canine lymphoma after a short L-CHOP protocol
Authors: Troedson Karin , Ignatenko Nataliia , Fejos Csilla , Zablotski Yury , Hirschberger Johannes 
Published: June 2021

Abstract
Objective A number of different rescue protocols for relapsed canine multicentric large-cell lymphoma have been described. The aim of this pilot study was to evaluate the efficacy of a maintenance treatment in dogs that experienced a second complete remission after a short L-CHOP-rescue protocol.
Material and methods Included in the study were dogs experiencing the first lymphoma relapse during a treatment-free period which were treated with a short L-CHOP protocol, achieved a complete remission and were afterwards treated with a continuous maintenance phase (MP) protocol. The L-CHOP protocol consisted of weekly treatments, with at least 3 additional treatments following complete remission. Thereafter the MP protocol with 2-week treatment intervals was conducted. It consisted of alternating oral home administration of different alkylating agents and one intravenously administered cytotoxic agent of a different mechanism of action. The dogs were presented either every 4 or 6 weeks for intravenous treatment and at this time a complete blood count was performed. The durations of the first remission, disease-free interval and overall survival time were evaluated.
Results A total of 20 dogs were included in the study. A median of 7 weekly applications were given before the treatment was switched to the MP protocol. During MP, 14 dogs were treated intravenously every 6 weeks and 6 dogs every 4 weeks. Haematological adverse events were mainly mild. During the L-CHOP-protocol, one septic event occurred, and 2 dogs were hospitalized due to gastrointestinal adverse events. No patient required hospitalization during the MP. Fifteen dogs completed at least one cycle in the MP and a median of 8.5 chemotherapeutic treatments were administered. The median disease-free interval was 264 days and the median overall survival time was 737 days.
Conclusion and clinical relevance The protocol was generally well tolerated. Since 5 patients showed disease progression during the first cycle of the MP, dogs should ideally be evaluated for minimal residual disease before being switched to the MP. The case number in the presented study was low and the treatment relatively heterogeneous. Therefore, more dogs have to be treated with the proposed protocol before general recommendations can be made.
Effects of Aging on Hemodynamic Kinetics in Different Intensities of Dynamic Exercise
Authors: Borghi-Ricardo Matheus , Simões Rodrigo Polaquini , Santos Daniel Augusto , Archiza Bruno , Borghi-Silva Audrey 
Published: June 2021

AbstractHealthy aging hemodynamics is known to exhibit a time-dependent loss of function. We aimed at verifying whether older men would have a slowed cardiac output and stroke volume dynamics in response to the onset (“on”) and on recovery (“off”) of exercise in comparison to young men. Twenty healthy active men (10 young and 10 older) were recruited. Participants performed an incremental cardiopulmonary exercise testing on a cycle ergometer, and on another day, 3 constant workload tests in different intensities. Compared to younger, older men exhibited a slower cardiac output and stroke volume dynamics in both on and off transients for all exercise intensities (all P < 0.05). During higher intensities, both younger and older men had slower hemodynamic kinetics compared to lower intensities (all P < 0.05). There was strong negative relationship between the time constant of cardiac output on-kinetics during high-intensity with maximal exercise performance in both groups (r = –0.88, P < 0.01). We interpret these findings to mean that healthy older men have slowed hemodynamic kinetics compared to younger, but this difference becomes less evident in higher intensities of exercise.
Exploring Nanoemulsions for Prostate Cancer Therapy
Authors: Srivastava Sushmita , Haider Md. Faheem , Ahmad Afroz , Ahmad Usama , Arif Muhammad , Ali Asad 
Published: June 2021

AbstractProstate carcinoma is typical cancer. It is the second most common cancer globally. The estimated new cases in 2020 was 191 930 and estimated deaths was 33 330. Age, family history, & genetic factors are major factors that drive prostate cancer. Although, for treating metastatic disease, the major therapies available are radiation,bisphosphonate, and palliative chemotherapy. But the major drawback is therapy is disease-driven and later becomes metastatic and requires treatment. The ability to revolutionize cancer treatment by major targeting vehicles via the exploration of nanoemulsion suggests a potential for cancer treatment. The unique property of a biphasic liquid dosage form called nanoemulsion to reach leaky tumor vasculature is due to its nano-meter oil-droplet size of 20–200 nm. Recent reporting on nanoemulsions disclose their embracing and lay alternative for re-purposing herbal and synthetic drugs and their combination especially for targeting prostate cancer formulating an obtainable nanomedicine. So, this article emphasizes the use of nanoemulsions incorporating therapeutic agents for successful and targeted delivery for prostate cancer.
Dolor sacroilíaco: Revisión actualizada del enfrentamiento diagnóstico
Authors: Blanco Enrique , Frías Sebastián , Álvarez Facundo , Fleiderman José 
Published: June 2021

ResumenEl dolor sacroilíaco es responsable de 15% a 30% de los cuadros de dolor lumbar bajo. El diagnóstico de esta patología es un desafío para el médico, debido a su compleja anatomía, el amplio diagnóstico diferencial, y las diversas etiologías que pueden provocar dolor en la articulación sacroilíaca. Una anamnesis ordenada y dirigida, asociada a un examen físico preciso, ayuda a orientar el diagnóstico. Las pruebas sacroiliacas específicas deben realizarse en aquellos pacientes con sospecha de dolor sacroilíaco, y deben interpretarse en conjunto y no de manera aislada. La resonancia magnética sirve para descartar otras causas de dolor lumbar bajo o diagnosticar casos de sacroileítis inflamatoria. La infiltración de la articulación es el gold standard para el diagnóstico, y debe realizarse en pacientes con alta sospecha de dolor sacroilíaco, por la anamnesis, examen físico, y tres o más pruebas sacroilíacas específicas positivas.