bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Urinary Kallidinogenase (HUK) Alleviates Myocardial Injury in Rats with Coronary Microembolism Through PI3K/Akt/FoxO1 Signaling Pathway.
Authors: Xie Jian , Lin Yunhua , Liu Guoqing , Nong Qingqing , Wu Bingling , Xie Yuqian , Li Tao , Li Lang 
Published: November 2021

Abstract
PurposeCoronary artery microembolization (CME) is a severe clinical complication that can cause myocardial infarction, induce myocardial inflammation and apoptosis, ultimately lead to cardiac dysfunction. Human Urinary Kallidinogenase (HUK), a glycoprotein found in human urine, its role and underlying mechanism in CME are still unclear. Therefore, our goal is to explore the effect of HUK on the PI3K/Akt/FoxO1 axis of CME in rats, determine whether it can restrain myocardial inflammation and apoptosis, and alleviate CME-induced myocardial injury.MethodsWe split 40 Sprague-Dawley (SD) rats into CME, CME + HUK, CME + HUK + LY294002 (CME + HUK + LY), and sham operation groups randomly (10 animals in each group). The dosage of HUK was 0.016 PNA/kg/day. Also, 42μm inert plastic microspheres were injected into the left ventricle of rats to establish the CME model. Notably, rats in the CME+HUK+LY group were injected 10 mg/kg of LY294002 (a particular inhibitor of PI3K) intraperitoneally 30 minutes before modeling. We measured cardiac function 12 hours after the operation. Besides, the serum of myocardial injury biomarkers and myocardial inflammation, as well as apoptosis-related genes were measured, the myocardial histopathological examination was also performed.ResultsOur results indicated that CME induced myocardial inflammation and apoptosis, also caused myocardial infarction. HUK mainly activated PI3K/Akt/FoxO1 signal transduction, effectively reducing myocardial inflammation, apoptosis, and myocardial infarction area and improving CME-induced myocardial injury. In addition, these cardioprotective effects can be reduced by PI3K specific inhibitor LY294002, suggesting that the above protective effects may be related to the process of activation of the PI3K/ Akt /FoxO1 axis.ConclusionOur findings revealed that HUK might restrain myocardial inflammation and apoptosis by activating the PI3K/Akt/FoxO1 axis, thus ameliorating CME-induced myocardial injury.
Transcriptome Analysis of Genes Involved in Aluminum Stress Responses in Peanut (Arachis Hypogaea L.)
Authors: Bao Gegen , Li Shengyu , Zhou Qi , Ashraf Umair , Qiao Jingxuan , Wan Xiaorong , Zheng Yixiong 
Published: November 2021

Abstract
Background

Aluminum (Al) contamination inhibits plant growth and development, however, mechanisms involved in Al stress tolerance in peanut (Arachis hypogaea L.) were rarely studied. The present study was comprised of four Al levels i.e., 0, 1.25, 2.5 and 5 mmol l−1 AlCl3.18H2O regarded as Al0, Al1, Al2, and Al3. The respective concentrations were added in Hoagland nutrient solution and replaced every three days.
Result

Results revealed that seeding length low Al concentration (Al1) treatment had no noticeable effect on seeding lenght, while higher Al concentration (Al2 and Al3) treatment significantly inhibited seeding lenght. The differentially expressed genes (DEGs) of plant hormone metabolism pathway were significantly enriched whereas the contents of salicylic acid (SA) and abscisic acid (ABA) were up-regulated, and jasmonic acid (JA) were down-regulated to different levels. Moreover, transcription factors (TFs) and ALMT9 and FRDL1 genes were up-regulated at higher Al concentration and down-regulated at the lowest Al concentration (Al1).
Conclusions

Overall, Higher Al concentrations up-regulated the expression of transcription factors (TFs), and ALMT9 and FRDL1 genes to resist the stress of high Al concentrations whereas transcriptome analysis revealed that Al stress tolerance is closely related to endogenous hormone contents i.e., salicylic acid (SA), abscisic acid (ABA), and jasmonic acid (JA). This study preliminarily analyzed the molecular mechanism of Al tolerance in peanut and provided a theoretical rationale for developing new Al-tolerant peanut cultivars.
Comparison of Laparoscopic and Open Emergency Surgery for Colorectal Perforation: A Retrospective Study
Authors: Kudou Kensuke , Kusumoto Tetsuya , Nambara Sho , Tsuda Yasuo , Kusumoto Eiji , Yoshida Rintaro , Sakaguchi Yoshihisa , Ikejiri Koji 
Published: November 2021

Abstract
Background

This study aimed to clarify the safety and efficacy of laparoscopic surgery for colorectal perforation by comparing the clinical outcomes between laparoscopic and open emergency surgery for colorectal perforation.
Methods

We retrospectively reviewed the data of 100 patients who underwent surgery for colorectal perforation. The patients were categorized into two groups: the open group included patients who underwent laparotomy, and the laparoscopic group included those who underwent laparoscopic surgery. Clinical and operative characteristics and postoperative outcomes were evaluated.
Results

The open and laparoscopic groups included 58 and 42 patients, respectively. More than half of the patients in both groups developed perforation in the sigmoid colon (open, 55.2%; laparoscopic, 59.5%). The most common cause of perforation was diverticulum, followed by colorectal cancer. The mean intraoperative blood loss tended to be lower in the laparoscopic group than in the open group (78.8 mL versus 160.1 mL; P=0.0756). Hospital stay tended to be shorter in the laparoscopic group than in the open group (42.5 versus 55.7 days; P=0.0965). There were no significant differences in either the short- or long-term outcomes between the two groups. Univariate and multivariate analyses showed that the choice of surgical approach (open versus laparoscopic) did not affect overall survival in patients with colorectal perforation.
Conclusions

The laparoscopic approach for colorectal perforation in an emergency setting is a safe procedure compared with the open approach. The laparoscopic approach was associated with a decrease in intraoperative blood loss and a shorter length of hospital stay.
Evaluation of level of expression of microRNA-21 and let-7g in serum and stool of patients with colorectal cancer
Authors: Rahmanpour Dara , Dolatkhah Roya , Navidizadeh Soheil , Virani Peyman , Hasanzadeh Nastran , Hashemzadeh Shahryar 
Published: November 2021

Abstract
Background

MicroRNAs (miRs) are involved in the pathogenesis of various malignancies such as colorectal cancer through regulating multiple cellular processes, including cell proliferation, cell cycle, apoptosis and migrationMiR-21 and let-7 are two important genes that have confirmed in this pathway. The role of the let-7 gene as a gene tumor process in various cancers and the role of miR-21 in the development and progression of cancer has been conclusively identified also this gene has an oncogenic role in various cancers. In this study, the expression patterns of miR-21 and let-7 in serum and stool samples of colorectal cancer patients were evaluated.
Materials and Methods

During the present study, 120 samples including 40 serum samples of CRC and 40 stool samples from the same patients and 40 healthy samples were collected. After total RNA extraction, real-time PCR was used to measure changes in genes expression. Statistical analysis of data was performed with GraphPad Prism statistical software (Version 6.0) with a significance level of 5%.
Results

The relative expression level of miR-21 in the serum samples of CRC increased compared to the healthy group, which was statistically significant. On the other hand, the relative expression level of let-7g in the serum samples of CRC showed a significant decrease compared to the healthy sample. In stool samples, the expression changes of either of the two genes were not significant.
Conclusion

Our findings indicate that the relative expression of miR-21 and let-7g genes can be used as a diagnostic or predictive biomarker in colorectal cancer serum samples. While, this is not the case in stool samples. Moreover, further investigations at the protein level should be performed.
Factors Associated with Non-Use of modern Contraceptives among sexually active women in Ethiopia: A multi-level mixed effect analysis
Authors: Mulugeta Solomon Sisay , Fenta Setegn Muche , Fentaw Kenaw Derebe , Biresaw Hailegebrael Birhan 
Published: November 2021

Abstract
Background: Appropriate contraceptive use prevents unintended pregnancy, protects the health of mother and child, and promotes women's well-being. Use of modern Family planning in Ethiopia was still very low. The purpose of this study was to assess the factors that are associated with non-use of modern family planning services among women of reproductive age. Method: A nationally representative 2016 EDHS women data were used for analysis. Descriptive and multilevel multivariable binary logistic regression models were used to summarize descriptive data and measure statistical association between the dependent and the individual and community level variable, respectively. Adjusted odds ratio and confidence interval were respectively used to measure association and its statistical significance. Result: Among women in the reproductive age group 79.49% did not use a modern contraceptive method. Of this, 65.9 %, were from rural areas. Women age between 25-34 years (AOR=0.54, 95% CI: 0.5-0.61) and age between 34-49 year (AOR=0.62, 95% CI: 0.55-0.71), Women with a primary level of education (AOR=0.8, 95% CI: 0.68-0.87), husband’s education level was secondary and above were 0.83(AOR = 0.83, 95% CI: 0.72-0.96), middle income women (AOR=0.66, 95% CI: 0.58-0.76) , rich women (AOR=0.74,95%CI:0.65-0.85), women delivered at the health facility were lower by 0.84 (AOR = 0.84, 95%CI: 0.73-0.0.98), watched family planning related information from TV (AOR=0.74, 95% CI: 0.65-0.85) had 1-2 and 3 or more children in the previous five years (AOR = 0.21, 95% CI: 0.19-0.23) and (AOR = 0.4, 95% CI: 0.28-0.56), respectively, are individual-level factors that are less likely to not use contraception were identified. Furthermore, Muslim women (AOR=1.4, 95% CI: 1.23-1.62), women living in rural area (AOR=3.43; 95% CI: 2.72-4.32), and ANC visit 1.3(1.07-1.5) were more likely to not use contraception. Further, Women in Afar, Somali, Gambela, Harari, and Dire Dawa were less likely to use modern contraception methods than women in Tigray, but Amhara region had a lower rate of non-use. Conclusion: Family planning interventions should target younger women, women living in rural areas, the poor, and Muslim women. Moreover, initiatives to empower women associated to family planning programs would be beneficial in increasing contraceptive uptake among sexually active women in Ethiopia.
Design and Development of a Micro-LDH Apparatus to Determine Formability of Sheet Metal Under Hot Stamping Conditions Using Gleeble
Authors: Singh Amarjeet Kumar , Narasimhan Krishnaiyengar 
Published: November 2021

Abstract
In the last few years, demand for hot stamped components has increased in the automotive industry. Determination of formability under hot stamping is challenging due to elevated temperature, fast cooling and high punch velocity. Although there was various strive for formability determination but had limitations with experiments like non-uniform heating of specimen, non-uniform strain and temperature distribution. Therefore, in this work, an experimental apparatus was developed to determine formability at room temperature, high temperature, hot stamping conditions, and any complex process cycle involving heating and cooling. New specimen was designed to produce different strain paths, uniform and homogenous temperature distribution with the help of FEM software using thermomechanical and thermoelectrical simulation. A micro hemispherical dome based experimental apparatus was designed using Solidworks. The designed apparatus was used in conjunction with the thermo-mechanical simulator (Gleeble-3800). Thermomechanical analysis was done in PAM STAMP software to optimize specimen size and shape to get uniform strain distribution and different strain paths. A thermoelectric FEM model was developed using Abaqus 6.14 to optimize the temperature distribution in the specimen. The developed model enables choosing the appropriate polarity of the electrical cable connection to achieve uniform temperature distribution in the specimen. Strain path and temperature profiles for experiment and simulation were compared. Further, a forming limit curve was developed using the designed apparatus to verify the feasibility of the apparatus. For feasibility test of apparatus, hot stamping process was selected. This new design apparatus can be used for a range of temperatures up to 1000 °C, hot stamping conditions, and for different materials (aluminium, magnesium alloys, different grade of steel, etc.). It concludes that the connection of different polarities of electrical cable was critical for homogenous and uniform temperature distribution in specimens.
Potential of two indigenous strains of entomopathogenic nematodes, (Steinernema feltiae and Heterorhabditis bacteriophora) against the tomato leafminer (TLM), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), under laboratory and greenhouse environmental bioassay
Authors: Kasi Indra Kumar , Singh Mohinder , Waiba Kanchhi Maya , Sharma P L Lal 
Published: November 2021

Abstract

The tomato leafminer (Tuta Absoluta) is an important pest of tomato crops which have features like; high reproductive potential, Invasive species are major threats to agronomic and natural ecosystems. Based on the pathogenicity of strains, only two isolates effectively show larvicidal activity. The native isolate was obtained from soil samples, collected from Rajgarh, Hamachi Pradesh, India. Petri dish bioassay use nematodes S. feltiae HR1 and H. bacteriophora HR2 species dose (0, 10, 20, 40, 80, 160/ IJs/cm2). The (%) 1st instar larval mortality was ranged from 24.15 to 85.38 %. 2nd instar from 29.87 to 90.00 %. 3rd instar from 24.15 to 90.00 %. 4th instar from 31.53 to 90.00 % and pupae stage mortality was ranged from 31.53 to 85.38 %. Larvicidal activity after 48- and 72-hours exposure, the S. feltiae and H. bacteriophora (1.0, 1.30, 1.60, 1.90, 2.20/IJs/cm2) showed potent larvicidal activity with LC50, LC75 and LC90 of all instars and pupa show high mortality. The strain inhibits the larval and pupal development 48 to 72 hr exposer time with LC50 range from 05.42 to 23.67, LC75 20.29 to 83.12, LC90 16.52 to 98.89. Green house test is using the seam isolate of EPNs (HR1, HR2) on foliar application it caused by significant mortality results. These studies demonstrate the challenge for invasive species. The local indigenous strains of EPNs (S. feltiae HR1, H. bacteriophora HR2) as a good biocontrol agent against invasive pest of T. absoluta.
Distributed Multi-Agent Learning is More Effectively than Single-Agent
Authors: Ke Shuya , Liu Wenqi 
Published: November 2021

Abstract
Interpretable distributed group intelligence techniques have emerged as an essential topic in artificial intelligence. The mathematical interpretability of prediction outcomes is critical for improving the reliability of machine learning, especially in random scenes. Although some experimental results published so far show that the prediction of group intelligence is better than individual intelligence, establishing a mathematical foundation for the superiority of distributed group intelligence is still a challenging problem for enhancing the interpretability of learning systems. Through the Radermacher complexity principle, we proved mathematically that the learning quality of group machine intelligence is better than its subset machine intelligence with a high probability, significantly better than any individual among them if the number of individuals in the group is large enough. We proposed a multi-agent distributed learning method for time series forecasting by incorporating multi-agent cooperation in cognitive processes into machine learning. In addition, since the way of cooperative interaction between multi-agent affects the training effect of the model, we provide a generalized interaction approach and prove its convergence. We conduct sufficient experiments on predicting time series for classically chaotic systems, and the results indicate that distributed group intelligence significantly improves the prediction accuracy of individual intelligence. The experiments result shows that the prediction error reduces substantially as the number of agents increases, confirming the theoretical accuracy and the model's validity. This work provides new ideas for theoretically exploring how group intelligence emerges.
Optical Coherence Tomography Angiography Measurements in Eyes with Vitreoretinal Surgery
Authors: Demir Mehmet 
Published: November 2021

Abstract
PurposeTo study the retinal detachment (RD) results with the aid of OCTAMethodsIn the present corss-sectionally designed study 11 patients were enrolled. Eyes with retinal detachment surgical procedures served as study group and the fellow eyes without any retinal detachment surgical procedures served as control group. ResultsThe mean age of 11 patients was 59.72±9.94 years. The mean BCVA of the study was 0.43±0.31 and mean BCVA of the control group was 0.86±0.23 in decimal (P=0.01). The mean IOP of the study group 15.72±1.55 mmHg and the mean IOP of the control group was 16.09±2.17 mmHg (P=0.656). ConclusionOptical coherence tomography angiography is an important imaging tool to evaluate retinal superficial and deep layer density and area measurements
Characterization and Sequencing of a Novel Phage Abp95 Against Multi-genotypes of Carbapenem-resistant Acinetobacter Baumannii
Authors: Huang Li , Huang Siyi , Jiang Lingli , Tan Jingjie , Yan Xueping , Gou Chunmei , Chen Xinchong , Xiang Lijuan , Wang Dali , Huang Gaungtao , Zhang Yixin 
Published: November 2021

Abstract
Acinetobacter baumannii has become a challenging conditional pathogen. A. baumannii can lead to different infections, such as wound or urinary tract infections and pneumonia. As an alternative strategy for antibiotic-resistant A. baumannii infections, phage therapy had been used and approved by several governments. Previously we had reported two potential phage therapy candidates named Abp1 and Abp9. In this study, a wide host range lytic phage Abp95 were isolated and sequenced. The biological characteristics of Abp95 are also stuied. Abp95 belongs to the myoviridae family, containing a G+C content of 38.07% with a genome of 43,176 bp. Abp95 genome encodes 77 hypothetical genes, without any known virulence genes. With a diabetic wound infection model, Abp95 could accelerate wound healing though clearing local infections of multidrug-resistant A. baumannii. In conclusion, wide host range lytic phage Abp95 shows the potential as phage therapy candidate against multi-genotypes of Carbapenem-Resistant Acinetobacter baumannii.
Variability of Climate-Induced Rice Yields in Northwest Bangladesh Using Multiple Statistical Modeling
Authors: I Abu Reza Md. Towfiqul , Nabila Irtifa Alam , Hasanuzzaman Md. , Rahman Md. Bozlar , Elbeltegi Ahmed , Mallick Javed , Techato Kuaanan 
Published: November 2021

Abstract
Because of evident climatic variations and significant contribution to national food production, Bangladesh is a climate extreme hotspot region of examination for climatic consequences for rice (Oryza Sativa) crop production. This study intends to explore the variability of climatic variables (e.g., variations in mean temperature, rainfall, relative humidity, and sunshine duration) with rice yields (e.g., Aus, Aman, and Boro rice varieties) in northwest Bangladesh. The modified Mann-Kendall test, Theil-Sen slope estimator, and multiple linear regression (MLR) modeling were used to estimate the association among these factors. Heteroskedasticity and autocorrelation constant standard error (HAC) and feasible generalized least square (FGLS) technique were adopted to measure the climate-rice crop nexus using the regional level dataset for 1976–2015. Furthermore, the spatiotemporal variation of rice yield trends with climatic variables was mapped and assessed by the coefficient of variation. The results show that observed temperature and humidity trends were beneficial for Aus and Aman yields but not Boro yields. In contrast, observed rainfall and sunshine trends were negative for all three rice seasons. The outcomes of the MLR model explained 67%, 92%, and 83% of the variability in Aus, Aman, and Boro rice yields in the study region. The model outcomes showed that humidity and rainfall have negatively affected Aus and Aman rice crops, while temperature and rainfall positively influence Boro rice yield. Regarding the climate change issues and safeguarding food safety at the regional level, the concerned authorities should provide substantial attention to improving heat and drought-tolerance high-yielding varieties against climate effects on Aus and Aman rice varieties.
Liver Transplantation Does Not Increase Morbidity or Mortality in Women Undergoing Surgery for Breast Cancer
Authors: Veillette Gregory , Castaldi Maria , Roberts Sacha , Parsikia Afshin , Choubey Ankur , Okumura Kenji , Latifi Rifat , Ortiz Jorge 
Published: November 2021

Abstract
Purpose

The incidence of breast cancer (BC) in solid organ transplant recipients is comparable to the age-matched general population. The rate of reported de novo breast cancer following liver transplantation (LT) varies. Further, there is limited information on the management and outcomes of breast cancer in liver transplant recipients (LTR). We aim to evaluate the impact of LT on breast cancer surgery outcomes. Further we compare the outcomes after breast cancer surgery in LTR in transplant versus non-transplant centers.
Methods

National Inpatient Sample (NIS) database was accessed to identify LTR with BC. Mortality, complications, hospital charges and total length of stay (LOS) were evaluated with multivariate logistic regression testing. Weighted multivariate regression models were employed to compare outcomes at transplant and non-transplant centers.
Results

Ninety-nine women met inclusion criteria for LT + BC and were compared against a cohort of women with BC without LT (n=736,527). LT + BC had lower performance status as confirmed via higher Elixhauser Comorbidity Index (20.5% vs.10.2%, p < 0001). There were significantly more complications in the LT cohort when compared to the non-LTR (15.0% vs. 8.2%, p=0.012). However, on multivariate analysis, LT was not an independent risk factor for post-operative complications following breast cancer surgery (OR 1.223, p=0.480). Cost associated with breast cancer care was significantly higher in those with LT (2.621, p<0.001). Breast conservation surgery in LT had shorter LOS as compared to BC alone (OR 0.568, p 0.027) in all hospitals.
Conclusion

LT does not increase short-term mortality when undergoing breast cancer surgery. Although there were significantly more complications in the LT cohort when compared to the non-LTR (15.0% vs. 8.2%, p=0.012), on multivariate analysis, LT was not an independent risk factor for postoperative complications following breast cancer surgery. Additionally, breast cancer treatment is more costly in LTR. Breast cancer management in LTR at non-transplant centers incurred higher charges but no difference in complication rate, nor LOS when compared to breast cancer management in LTR at transplant centers.