bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promiscuous Behavior of HPV16E6 Specific T Cell Receptor Beta Chains Hampers Functional Expression in TCR Transgenic T Cells, Which Can Be Restored in Part by Genetic Modification
Authors: Scholten Kirsten B. J. , Ruizendaal Janneke J. , Graf Marcus , Schoedl Thomas , Kramer Duco , Meijer Chris J. L. M. , Man Stephen , Hooijberg Erik 
Published: January 2021

Background: T cell receptor gene transfer is a promising strategy to treat patients suffering from HPV induced malignancies. Therefore we isolated the TCRαβ open reading frames of an HPV16E6 specific CTL clone and generated TCR transgenic T cells. In general low level expression of the transgenic TCR in recipient human T cells is observed as well as the formation of mixed TCRs dimers. Here we addressed both issues employing three different expression platforms.Methods: We isolated the HVP16E6 specific TCRα and TCRβ open reading frames and retrovirally transduced human T cells with either wild-type (wt), or codon-modified (cm) chains to achieve enhanced TCR expression levels, or used codon-modification in combination with cysteinization (cmCys) of TCRs to facilitate preferential pairing of the introduced TCRα and TCRβ chains.Results: Careful analysis of recipient T cells carrying the HPV16E6 TCRβ and endogenous TCR chains revealed the transgenic TCRβ chain to behave very promiscuously. Further analysis showed that the percentage of tetramer positive T cells in codon-modified/cysteinized TCR transgenic T cells was four-fold higher compared to wild-type and two-fold higher compared to codon-modification only. Functional activity, as determined by IFN-γ production, was high in cmCysTCR transgenic T cells, where it was low in cm and wt TCR transgenic T cells. Recognition of endogenously processed HPV16E6 antigen by cmCysTCR transgenic T cells was confirmed in a cytotoxicity assay.Conclusions: Promiscuous behavior of the HPV16E6 specific TCRβ chain can in part be forced back into specific action in TCR transgenic T cells by codon modification in combination with the inclusion of an extra cysteine in the TCR chains.
Association of Vitamin D Deficiency and Newly Diagnosed Pulmonary Tuberculosis
Authors: Jaimni Vijay , Shasty Barkur Ananthakrishna , Madhyastha Sharath P. , Shetty Ganesh V. , Acharya Raviraja V. , Bekur Ragini , Doddamani Akhila 
Published: January 2021

Introduction. Vitamin D has a significant role in host immune defense against Mycobacterium tuberculosis. It has been suggested that pulmonary tuberculosis may be associated with lower levels of vitamin D. Present study was therefore undertaken to identify the association between vitamin D deficiency and pulmonary tuberculosis. Methods. A case-control study was conducted in a tertiary care hospital from 2014 to 2016, including 50 adult newly diagnosed sputum positive pulmonary tuberculosis patients as cases and 50 age and sex-matched healthy participants as control groups. All participants in the study group had undergone detailed clinical examination and routine laboratory investigations, including vitamin D, calcium, and sputum for AFB. The clinical characteristics, X-ray findings, sputum AFB, and vitamin D levels were analyzed and compared with data obtained from healthy controls. Results. In both groups, the majority were men (88%). BMI was significantly (<0.0001

∗

) lower in the tuberculosis group (19.40 (17.20, 22.0) vs. 24.00 (22.50, 25.47)). Serum vitamin D levels were significantly lower (

P
=
0.012

) in the tuberculosis group (19 (7.75, 27.25) ng/dl) as compared to the control group (25 (19.75, 32.00) ng/dl). Out of 50 TB patients, 27 (54%) had vitamin D deficiency, while among healthy controls, only 13 (26%) had vitamin D deficiency. Among vitamin D deficient PTB patients, 44% had 3+/hpf AFB in sputum smear examination. Conclusion. The prevalence of vitamin D deficiency in pulmonary tuberculosis cases is very high. Hypovitaminosis D was associated with more severe clinical symptoms, higher sputum smear positivity, and extensive lesions in chest radiograph among pulmonary tuberculosis patients.
Effects of Heat Addition on Wave Drag Reduction of a Spiked Blunt Body
Authors: Wang Hongyu , Yang Yanguang , Li Langquan , Wang Gang , Zhang Qinghu 
Published: January 2021

Drag reduction technology plays a significant role in extending the flight range for a high-speed vehicle. A wave drag reduction strategy via heat addition to a blunt body with a spike was proposed and numerically validated. The heat addition is simulated with continuous heating in a confined area upstream of the blunt body. The effects of heat addition on drag reduction in three flow conditions (

M
=
3.98
,
5
,
6

) were compared, and the influence of power density

q

h

(

q

1

=
2.0
×

10

8

W
/

m

3

,

q

2

=
5.0
×

10

8

W
/

m

3

, and

q

3

=
1.0
×

10

9

W
/

m

3

) of heating was evaluated. Results show that the heat addition has a positive way to reduce the drag of the body with a spike alone, and more satisfactory drag reduction effectiveness can be achieved at a higher Mach number. The drag reduction coefficient increases with

q

h

in the same flow condition, with a maximum of 38.9% (

M
=
6

) as

q

3

=
1.0
×

10

9

W
/

m

3

. The wave drag reduction principle was discussed by a transient calculation, which indicates that the separation region has entrainment of the heated air and expanded with its sonic line away from the blunt cone, which results in an alleviation of the pressure load caused by shock/shock interaction.
Quantitation of Apremilast in Beagle Dogs Plasma by UPLC-MS-MS and Its Application to Pharmacokinetic Studies
Authors: Xiong Wei , Wang Ling , Zhang Haiyan , Tao Xiaoqiu , Jiang Xuehua , Liu Zejuan , Zhao Jiajia , Xu Wenwen 
Published: January 2021

A sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method for the determination of apremilast in beagle dog plasma has been developed and successfully validated in the current study. Clopidogrel was employed as an internal standard (IS), and liquid-liquid extraction by tert-butylmethyl ether was used for sample preparation. Chromatographic separation was achieved on a UPLC BEH Shield RP18 column (50 mm × 2.1 mm, 1.7 μm) with 5 mM ammonium formate water and 5 mM ammonium formate methanol as the mobile phase with gradient elution. Calibration plots were linear in the range of 2–3000 ng/mL for apremilast in beagle dog plasma. Mean recoveries of apremilast in beagle dogs plasma ranged from 87.4% to 97.4%. The intrarun and interrun precision was less than 6% and 9%, respectively, with the accuracy between 92.4% and 101.1%. The method has also been successfully applied in the pharmacokinetics study of apremilast. The mean t1/2Z was 5.41 h for 30 mg·day−1 for beagle dogs after oral administration. The AUC0-t increased linearly from 3.51 to 1802.13

μ
g

L

−
1

∗
h

after administration of single doses.
Habitation Altitude and Left Ventricular Diastolic Function: A Population‐Based Study
Authors: Zheng Congyi , Wang Xin , Tang Haosu , Chen Zuo , Zhang Linfeng , Wang Su , Kang Yuting , Yang Ying , Jiang Linlin , Huang Gang , Wang Zengwu 
Published: January 2021

Background
Although numerous studies have been published evaluating the positive or negative effects of altitude on cardiovascular disease, many of them are conflicting.

Methods and Results

Data come from 2 cross‐sectional surveys using a similar method in China; and a total of 34 215 residents, aged ≥35 years, were eligible and recruited in the study. Left ventricular diastolic dysfunction (LVDD), according to the 2009 American Society of Echocardiography guidelines, was defined and evaluated. Altitude was divided into low (<1500 m), middle (1500–3500 m), and high (≥3500 m) level groups. Among the 34 215 participants (aged 55.87 years; men, 45.92%; altitude ranging from 3.1 ~ 4507 m), 15 099 (crude prevalence, 44.13%), 517 (crude prevalence, 1.51%), and 272 (crude prevalence, 0.79%) were diagnosed as having grades I, II, and LVDD, respectively. Compared with low‐level group, the odds ratios (ORs) (95% CIs) of LVDD for middle‐ and high‐level groups were 1.65 (1.49–1.82) and 1.89 (1.63–2.19), respectively (
P
trend
<0.001). The ORs (95% CI) were 1.43 (1.31–1.56) and 2.03 (1.67–2.47) per 500‐m increment for middle‐ and high‐level groups. There was a nonlinear relationship (upward‐sloping “W” shape) between altitude and the risk of LVDD, assessed by the restricted cubic spline. For each LVDD grade, ORs (95% CIs) of grade I LVDD for middle‐ and high‐level groups were 1.75 (1.59–1.92) and 1.95 (1.69–2.25), respectively; for grade II, ORs (95% CIs) for middle‐ and high‐level groups were 6.19 (3.67–10.42) and 5.27 (2.18–12.74), respectively. The stratified analyses indicated that LVDD was much more remarkably influenced by elevated altitude in men (
P
interaction
=0.0019).

Conclusions
Higher altitude is associated with increased risk of LVDD among people living over 1500 m, especially for men.
Are tyrosine kinase inhibitors fit for purpose in the treatment of metastatic papillary renal cell carcinoma?
Authors: Hemmant Joshua , Chow Karyee 
Published: January 2021

Renal cell carcinoma encompasses a range of histological subtypes. The treatment of metastatic disease in this context remains challenging. Papillary renal cell carcinoma is the second most common subtype and forms a significant subsection of non-clear cell renal cell carcinoma. Tyrosine kinase inhibitors form a significant part of the treatment of this largely incurable disease; however, outcomes tend to be poor. The aim of this article is to scrutinise whether these treatments are evidence-based in their use for metastatic papillary renal cell carcinoma and if good outcomes are reported. A literature review was made using PubMed of major prospective and retrospective studies. The European Association of Urology and European Society of Medical Oncology have both published guidance suggesting sunitinib should be offered first line in the treatment of metastatic papillary renal cell carcinoma. This, however, is based upon weak evidence produced by the ASPEN trial, although this did not discriminate between non-clear cell subtypes and results in further studies were modest. The National Institute for Care and Health Excellence has recently published new guidelines for the use of cabozantinib, which has shown evidence of improved progression-free survival and overall response rates compared with sunitinib. Unfortunately, many of the relevant studies did not specifically assess these treatments in patients solely with papillary renal cell carcinoma and had modest overall success. There is weak evidence that tyrosine kinase inhibitors give significant benefit in those patients with metastatic papillary renal cell carcinoma. Level of evidence: 2a
Synovial sarcoma of the hand-wrist: a case report and review of the literature
Authors: Serinelli Serenella , Gitto Lorenzo , Zaccarini Daniel J. 
Published: January 2021

Abstract
Background
Synovial sarcomas are infrequent malignant tumors occurring mostly in adolescents and young adults. The occurrence of synovial sarcoma in the hand-wrist area is rare (4 to 8.5% of all synovial sarcomas in different studies).

Case presentation
This report documents an uncommon case of synovial sarcoma occurring in the hand-wrist of a 69-year-old Caucasian woman. She was subsequently treated with surgical excision and radiotherapy without recurrence after follow up.

Conclusions
This paper aims to characterize the demographic, pathologic, and clinical features with a literature review. The present literature review confirms that hand-wrist synovial sarcomas are more frequent among males and subjects 10 to 40 years old. Most cases in this location are usually not larger than 5 cm in size. The five-year survival rate is higher than that reported in a previous review on hand synovial sarcomas, and this suggests an improved survival in recent decades.
3D Printing as a Promising Tool in Personalized Medicine
Authors: Vaz Vanessa Marcia , Kumar Lalit 
Published: January 2021

AbstractPersonalized medicine has the potential to revolutionize the healthcare sector, its goal being to tailor medication to a particular individual by taking into consideration the physiology, drug response, and genetic profile of that individual. There are many technologies emerging to cause this paradigm shift from the conventional “one size fits all” to personalized medicine, the major one being three-dimensional (3D) printing. 3D printing involves the establishment of a three-dimensional object, in a layer upon layer manner using various computer software. 3D printing can be used to construct a wide variety of pharmaceutical dosage forms varying in shape, release profile, and drug combination. The major technological platforms of 3D printing researched on in the pharmaceutical sector include inkjet printing, binder jetting, fused filament fabrication, selective laser sintering, stereolithography, and pressure-assisted microsyringe. A possible future application of this technology could be in a clinical setting, where prescriptions could be dispensed based on individual needs. This manuscript points out the various 3D printing technologies and their applications in research for fabricating pharmaceutical products, along with their pros and cons. It also presents its potential in personalized medicine by individualizing the dose, release profiles, and incorporating multiple drugs in a polypill. An insight on how it tends to various populations is also provided. An approach of how it can be used in a clinical setting is also highlighted. Also, various challenges faced are pointed out, which must be overcome for the success of this technology in personalized medicine.
Gerardo CAETANO y Fernando MAYORGA (comps.). Giros políticos y desafíos democráticos en América Latina: enfoques de casos nacionales y perspectivas de análisis. Buenos Aires: CLACSO, 2020. 245 pp. ISBN: 978-987-722-579-2.
Authors: Silva Marcos Antonio da 
Published: January 2021

És una reseña de la obra: Caetano, Gerardo y Mayorga, Fernando (comp.). Giros políticos y desafíos democráticos en América Latina: enfoques de casos nacionales y perspectivas de análisis. Buenos Aires: CLACSO, 2020.
PENDEKATAN GROUP WORK DALAM PRAKTIK PEKERJAAN SOSIAL: PENGALAMAN PEKERJA SOSIAL DI LEMBAGA KESEJAHTERAAN SOSIAL (LKS) DI INDONESIA
Authors: Napsiyah Siti , Zaky Ahmad 
Published: January 2021

Abstract. Group work in social work practice is one of methods for social workers’ intervention to help the client to cope with their social problems. This research aims to discuss the importance of group work method in social work practices: How they use group work methods, what are the steps of group development process; what are ethical and cultural issues occur during the process? Research method used is qualitative method. Data collection of the research is throughout interview as well as online survey. This research uses also document analysis throughout search engine such as google scholar and google form. The result of the study shows that group work becomes strategic method for social workers to help the client to solve their problems. This study also confirms social workers in social welfare institutions (LKS) in Indonesia are mostly use group work method. Interestingly, this research discuss the issue of cultural and religious ethics in the context of group process in Muslim group and community. Abstrak. Pendekatan group work dalam praktik pekerjaan sosial merupakan salah satu pendekatan yang menjadi andalan praktik profesi pekerja sosial dalam memberikan pertolongan kepada klien (individu, kelompok, komunitas, dan organisasi) dalam menyelesaikan masalah. Penelitian ini berusaha untuk menjelaskan tentang metode group work dalam praktik pekerjaan sosial, bagaimana pekerja sosial menggunakan metode group work dalam menyelesaikan permasalahan klien, bagaimana dinamika kelompok muncul dalam proses group work, dan mendiskusikan tentang bagaimana isu etik dan sensitivitas budaya muncul saat proses pelaksanaan group work. Jenis penelitian yang dipilih adalah penelitian kualitatif deskriptif. Studi dokumentasi dilakukan dengan menelusuri sumber-sumber pada mesin pengindeks dan data base yang mendukung open access, seperti google cendekia (google scholar), academia.edu, dan lain-lain. Penelitian juga dilakukan dengan menggunakan survey kepada pekerja sosial melalui google form. Hasil penelitian menunjukkan bahwa metode group work dinilai sebagai pendekatan yang sangat penting bagi pekerja sosial dalam menyelesaikan permasalah sosial yang bersifat kelompok. Hasil penelitian ini juga menunjukkan bahwa dalam proses pelasaksanaan metode group work terdapat dinamika kelompok dan sensitivitas sosial dan agama. Sehingga isu integrasi keilmuan, keislaman dan keindonesiaan menjadi menarik dalam diskusi paper ini.
KEHIDUPAN ANAK TERLANTAR DI LKSA YUDA MANDIRI SOMBA OPU
Authors: Syamsudin Syamsudin 
Published: January 2021

Abstract. The life of an abandoned child certainly needs protection so that he can be independent. The article aims to analyze the living conditions of neglected children who have been coached by social workers and supervisors at the Yuda Mandiri Somba Opu Child Welfare Institution. The type of research is descriptive qualitative, obtaining data from informants through in-depth interviews and observations, to analyze data by way of data reduction, data presentation and drawing conclusions. The results obtained regarding the life of street children are the pre-Karangtina situation through assessment, the availability of dormitories, in school, mental guidance and moral guidance, group and individual recitation in the afternoon. Abstrak. Kehidupan anak terlantar tentu membutuhkan perlindungan agar ia bisa mandiri. Artikel bertujuan untuk menganalisi kondisi kehidupan anak terlantar yang selama ini di bina para pekerja sosial dan pembina Lembaga Kesejahteraan Sosial Anak Yuda Mandiri Somba Opu. Adapun jenis penelitian adalah deskriptif kualitatif, perolehan data dari informan melalui wawancara mendalam maupun observasi, untuk menganalisis data dengan cara reduksi data, penyajian data dan penarikan kesimpulan. Hasil yang diperoleh tentang kehidupan anak jalanan yaitu situasi pra karantina melalui assessment, tersedianya asrama tempat tinggal, di sekolahkan, pendampingan bimbingan mental dan pembinaan akhlak, pengajian di sore hari secara berkelompok maupun individu.
PROGRAM PENDIDIKAN PENGEMBANGAN KEPEMIMPINAN SEBAGAI UPAYA PEMBERDAYAAN PADA PEMUDA
Authors: Arief Aqil Wilda , Rahayu Ety 
Published: January 2021

Abstrak. This study attempts to see the implementation of leadership development education programs as an effort to empower youth as an effort to empower youth. The leadership development education program that was highlighted was a leadership development program implemented by the non-profit organization named Rumah Kepemimpinan. This study will discuss how the implementation of leadership development education programs is one of the steps to empower youth, especially in capacity building related to aspects of knowledge, skills and attitudes. This study is taken with a qualitative approach that will involve a number of informants from internal organizations and beneficiaries. From the results obtained, the beneficiaries felt significant results, especially in the aspect of capacity building in the aspects of knowledge, skills and attitudes that were more optimal, targeted and measured. Abstrak. Studi ini mencoba untuk melihat pelaksanaan program pendidikan pengembangan kepemimpinan sebagai upaya pemberdayaan pemuda sebagai salah satu upaya dalam pemberdayaan pemuda. Program pendidikan pengembangan kepemimpinan yang disoroti adalah program pengembangan kepemimpinan yang dilaksanakan oleh organisasi non-profit Rumah Kepemimpinan. Studi ini akan membahas bagaimana pelaksanaan program pendidikan pengembangan kepemimpinan menjadi salah satu langkah pemberdayaan pemuda terutama pada peningkatan kapasitas yang berhubungan dengan aspek pengetahuan, keterampilan dan juga sikap. Studi ini diambil dengan pendekatan kualitatif yang akan melibatkan sejumlah informan dari internal organisasi dan peserta program. Dari hasil yang didapat, peserta program merasakan hasil yang signifikan terutama dalam aspek peningkatan kapasitas pada aspek pengetahuan, keterampilan, dan sikap yang lebih optimal, terarah dan terukur.
PEMBANGUNAN PARTISIPATIF DI KOTA TANGERANG SELATAN MELALUI PROGRAM TANGSEL YOUTH PLANNER
Authors: Novian Muhammad Nurman , Machdum Sari Viciawati 
Published: January 2021

Abstract. Participatory development has become one of the main keys in development in accordance with what the community wants and needs. Community in participatory development is also a major highlight in the context of success in development. In participatory development in the City of South Tangerang, it is organized by the Tangsel Youth Planner (TYP) Program, which is the result of a collaboration between the South Tangerang City Development Planning Agency and the local community, namely the Tangsel Creative Foundation, which contains South Tangerang youth or local millennials. In previous research, it was said that the barrier to community participation was due to the lack of participation forums such as the Tangsel Youth Planner Program. From the results of this study it was found that it is very important for local community participation in development such as the Tangsel Youth Planner program. It is also known that the Tangsel Youth Planner program has the characteristics of an interventionist social development and uses a social development approach by the government. Abstrak. Pembangunan partisipatif sudah menjadi salah satu kunci utama dalam pembangunan yang sesuai dengan apa yang di kehendaki dan dibutuhkan oleh masyarakat. Masyarakat dalam pembangunan partisipatif juga menjadi sorotan utama dalam konteks keberhasilan dalam pembangunan. Dalam pembangunan partisipatif di Kota Tangerang Selatan di wadahi oleh Program Tangsel Youth Planner (TYP) yang merupakan hasil kerjasama Bappeda Kota Tangerang Selatan dengan Komunitas Lokal yaitu Tangsel Creative Foundation, berisi anak-anak muda Tangerang Selatan atau milenial lokal. Pada penelitian sebelumnya dikatakan bahwa hambatan partisipasi masyarakat dikarenakan minimnya wadah partisipasi seperti Program Tangsel Youth Planner. Dari hasil penelitian ini didapati bahwa sangatlah penting wadah partisipasi masyarakat lokal dalam pembangunan seperti adanya program Tangsel Youth Planner. Serta diketahui bahwa program Tangsel Youth Planner mempunyai karakteristik pembangunan sosial yang bersifat intervensionis, serta menggunakan pendekatan pembangunan sosial oleh pemerintah.
Prevalence of Neonatal Jaundice in the Zone Hospital of Suru-Lere at Cotonou (Benin)
Authors: Nicolas AÏKOU , DRAME Sidiki Boubacar , LAPO Rock Allister , Théodora AHOYO Angèle , MOUTAWAKILOU Gominan , Amadou COULIBALY Founzégué , Mirèze AÏKOU Nadine Lucrèce , Emérentienne AÏKOU Arielle Nadège , ZANNOU Lydie , ALKOARE Ibrahim , Lamine BABAMOUSSA 
Published: January 2021

Objectives: Summarize the data for better management of jaundice in neonatology Patients and methods: This was a study on 842 Beninese newborns aged 0 to 7 days hospitalized over the period from June 2014 to June 2015. Results: On 842 newborns, 170 were neonatal jaundice franc is 20.19%. The ratio is in favor of boys (1.8); jaundice is early in 2.35% of cases. Etiologies are represented by physiological jaundice, infection, maternal-fetal incompatibility erythrocyte. The use of exchange transfusion was required in 5.29% of cases. The rest of the treatment was dominated by intensive phototherapy and / or associated with conventional etiological treatment. Prematurity and infection are the main risk factors identified in our series. Conclusion: Jaundice is a common condition of course, but we draw attention to the importance of the rate of neonatal jaundice in our context related primarily to the non-monitoring of pregnancies, early discharge of maternity and consultations late.
Tags: jaundice, prevalence, newborn, spectrophotometer, bilirubin.