bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial phylogeny and taxonomic revision of Italian and Slovenian fluvio-lacustrine barbels, Barbus sp. (Cypriniformes, Cyprinidae)
Authors: Rossi Giovanni , Plazzi Federico , Zuffi Gianluca , Marchi Andrea , De Bonis Salvatore , Valli Marco , Marinšek Petra , Falconi Rosanna 
Published: April 2021

Abstract
Background
Barbels are ray finned cyprinid fishes of the Old-World with partially unresolved, intricate taxonomy. Within the Barbus sensu lato paraphyletic assemblage, Barbus sensu stricto is a monophyletic tetraploid lineage of Europe, northern Africa and Middle East, including two monophyletic sibling genera: Barbus and Luciobarbus. Italy, Slovenia and northern Croatia are natively inhabited by several entities of the genus Barbus, whose relationships and taxonomic ranks are still unclear. Aim of the present work is to focus on phylogeography of Italian and Slovenian barbels, with an appraisal of their current taxonomy.

Results
One hundred fifty specimens were collected in 78 sampling sites from 33 main watersheds, widely distributed along Italian and Slovenian ichthyogeographic districts. We amplified two mitochondrial markers, cytochrome b (cytb) and control region (D-loop), to infer a robust phylogeny for our sample and investigate on species delimitation.
Our results strongly indicate all Italian and Adriatic Slovenian fluvio-lacustrine barbels to be comprised into at least three distinct species. We provide a proposal of taxonomic revision and a list of synonymies for two of them and a new description under the International Code of Zoological Nomenclature rules for the third one.

Conclusions
If nuclear data will confirm our findings, at least three specific entities should be acknowledged across our sampling area. Namely, the three species are (i) Barbus plebejus, in the Padano-Venetian district; (ii) Barbus tyberinus, in the Tuscany-Latium district; (iii) Barbus oscensis Rossi & Plazzi sp. nov., in the Tyrrhenian and southernmost-Adriatic parts of Apulia-Campania district. Finally, we briefly discuss the implications of such a taxonomic scenario on conservation policies.
Design and development of semi-automatic concept map authoring support tool
Authors: Pinandito Aryo , Prasetya Didik D. , Hayashi Yusuke , Hirashima Tsukasa 
Published: April 2021

AbstractThis research, to design and develop a concept map authoring support tool, adopts a semi-automatic concept mapping approach to help teachers create concept maps from English readings. A concept map is widely regarded as a useful teaching and learning tool. It offers many potential advantages apart from representing the students’ knowledge and understanding during learning. Students’ engagement in using and creating concept maps with a computer-enabled concept mapping tool raises concept maps’ potential benefits. It contributes to the learning process and improves the students’ meaningful learning. The Kit-Build concept map framework, which incorporates a technology-enabled concept mapping tool, uses concept map recomposition as its essential learning activity. In learning with Kit-Build, teachers compose concept maps that they want the students to achieve. The teachers’ maps are then decomposed into components from which the students recompose and reflect deeply on their understanding. The difference between teacher’s and students’ concept maps depicts the gap between teachers’ expected understanding and students’ actual understanding. Hence, the teachers’ concept map becomes an essential part of learning with Kit-Build. For some teachers, creating a good concept map for learning is difficult and time-consuming. Hence, support to improve teachers’ productivity in creating concept maps is essential. The findings suggest that the support tool yields better concept mapping efficiency while maintaining concept maps of similar quality. Teachers also found that the support tool was useful. Therefore, semi-automatic concept mapping with the supported Kit-Build concept map authoring tool has been shown to be a better approach.
Post-stroke fatigue: an exploratory study with patients and health professionals to develop a patient-reported outcome measure
Authors: Skogestad Ingrid Johansen , Kirkevold Marit , Larsson Petra , Borge Christine Råheim , Indredavik Bent , Gay Caryl L. , Lerdal Anners 
Published: April 2021

Abstract
Background
Post-stroke fatigue (PSF) is commonly reported and described as disabling by patients recovering from stroke. However, a major challenge is how to accurately diagnose and assess PSF. Therefore, the aim of this study was to explore PSF as it is experienced by stroke survivors and described by health professionals to guide future development of a PSF-specific PROM.

Methods
Individual semi-structured interviews were conducted with stroke survivors experiencing PSF (n = 9) and three focus groups were conducted with health professionals (n = 16). Data were analyzed through inductive content analysis.

Results
The analysis revealed four themes illustrating the experience and descriptions of PSF: 1) PSF characteristics, 2) interfering and aggravating factors, 3) management, and 4) PSF awareness, which refers to stroke survivors first becoming aware of PSF after their initial hospital admission.

Conclusion
This study highlights the complexity and multidimensionality of PSF. The results from this study will guide future development of a PSF-PROM and support its content validity.
Dysregulation of CCAAT/enhancer binding protein-alpha (CEBPA) expression in the bone marrow of acute myeloid leukemia patients
Authors: Hassan Naglaa M. , Said Fadwa , Shafik Roxan E. , Abdellateif Mona S. 
Published: April 2021

Abstract
Background
Acute myeloid leukemia (AML) is a heterogeneous malignant disease characterized by accumulation of different types of mutations commonly the CCAAT/enhancer binding protein-alpha (CEBPA). However, the dysregulations of CEBPA expression in AML is still a debatable issue. The aim of the current study was to assess CEBPA gene expression in bone marrow (BM) aspiration specimens of 91 AML patients, compared to 20 control donors of bone marrow transplantation (BMT), using RT-PCR. Data were correlated with patients’ clinico-pathological features, response to treatment, progression-free survival (PFS), and overall survival (OS) rates.

Results
There was overexpression of CEBPA gene in AML patients compared to normal control [1.7 (0.04–25.6) versus 0.17 (0–4.78), respectively, P < 0.001]. Upregulation of CEBPA expression associated significantly with increased BM hypercellularity, total leucocyte counts, peripheral blood blast cell count, and poor PFS (P < 0.001, 0.002, 0.001, and 0.013, respectively). There was no significant association between CEBPA expression and any other relevant clinico-pathological features or OS rates (P = 0.610) of the patients. ROC analysis for biological relevance of CEBPA expression with AML showed that sensitivity and specificity of CEBPA expression at a cut-off value of 0.28 are 92.3% and 78.6%, respectively (P < 0.001). All patients who had CEBPA overexpression and mutant FLT3 showed BM hypercellularity, adverse cytogenetic risk, increased TLC, and PB blast cells count (P = 0.007, P < 0.001, 0.016, and 0.002, respectively).

Conclusion
CEBPA overexpression could be used as a genetic biological marker for AML diagnosis, as well as a poor prognostic factor for disease progression. It has no impact on OS rates of the patients.
Perceived parenting attitudes in relation to ADHD symptoms and aggressive behaviors in a sample of Egyptian children with ADHD
Authors: Azzam Hanan , Hasan Haytham , Elhabiby Mahmoud , El Ghamry Reem , Mansour Mona , Elhamshary Mohammed 
Published: April 2021

AbstractBackgroundExternalizing behaviors are common in children with attention-deficit hyperactivity disorder (ADHD). This study was aiming to investigate the perceived parenting attitudes in children with ADHD and their correlates to the severity of symptoms and associated aggressive behaviors in those children. Accordingly, 140 children with ADHD were assessed using Mini International Neuropsychiatric Interview for Children; Conners’ Parent Rating Scale-Revised, Long Version; the problem scale of the Child Behavior Checklist for ages 4–18; and the Perceived Parenting Attitude Scale.ResultsAssessment of associated behavioral problems in the participants revealed the presence of significant aggressive and externalizing problems. The participants perceived a similar attitude between their mothers and fathers. No significant association is found between severity of ADHD symptoms and perceived parenting attitudes, while consistency in parenting in both mothers and fathers was positively correlated to the aggressive behaviors of the participants.ConclusionsAggression is a common symptom associated with ADHD that should be identified and addressed. Although the current study has revealed that consistency in parenting is positively correlated to the aggression in ADHD children, yet children with ADHD might have a positive illusory bias in their social self-perceptions making it difficult to account for the reliability of their perceived parenting attitudes; hence, it remains to be clarified whether perceived parents’ attitudes are attributed to children’s aggression or to children’s perception caused by the previously mentioned illusory bias. For future research, it is highly recommended to assess parenting from the parents’ and their children’s points of view.
Stop! Go! What Can We Learn About Family Planning From Birth Timing in Settler South Africa, 1835–1950?
Authors: Cilliers Jeanne , Mariotti Martine 
Published: April 2021

Abstract
We revisit the discussion on family limitation through stopping and spacing behavior before and during the fertility transition with a sample of 12,800 settler women's birth histories in nineteenth- and twentieth-century South Africa. Using cure models that allow us to separate those who stop childbearing from those who continue, we find no evidence of parity-specific spacing before the transition. We do find evidence of non-parity-based birth postponement before the transition. Increased stopping and parity-independent postponement characterized the beginning of the fertility transition, with increased parity-specific spacing following later in the transition phase.
Phosphagen and Creatine in Frog’s Eggs
Authors: Andrzej Zielinski Mirosław 
Published: April 2021

ABSTRACT
In most papers on phosphagen published to date, its presence has been considered to be connected with the function of motion. This point of view is no longer tenable, since phosphocreatine has been found not only in every muscular tissue of the Vertebrata and in spermatozoa, but also in nerves, brain and tumours; labile phosphorus has recently been found in the eggs of Amphibia (Zieliński, 1935).
How Polarization-Sensitive Interneurones of Crickets Perform at Low Degrees of Polarization
Authors: Labhart T. 
Published: April 2021

ABSTRACT
In crickets, polarized-light information from the blue sky is processed by polarization-opponent interneurones (POL-neurones). These neurones receive input from the polarization-sensitive blue receptors found in the specialized dorsal rim area of the compound eye. Even under optimal conditions, the degree of polarization d does not exceed 0.75 in the blue region of the spectrum and it is normally much lower. The aim of this study is to assess how POL-neurones perform at low, physiologically relevant degrees of polarization. The spiking activity of POL-neurones is a sinusoidal function of e-vector orientation with a 180 ° period. The modulation amplitude of this function decreases strongly as the degree of polarization decreases. However, our data indicate that POL-neurones can signal e-vector information at d-values as low as 0.05, which would allow the polarization-sensitive system of crickets to exploit polarized light from the sky for orientation even under unfavourable meteorological conditions.
Design of the Oxygen and Substrate Pathways: VII. Different Structural Limits for Oxygen and Substrate Supply to Muscle Mitochondria
Authors: Weibel Ewald R. , Taylor C. Richard , Weber Jean-Michel , Vock Ruth , Roberts Thomas J. , Hoppeler Hans 
Published: April 2021

ABSTRACT
This paper integrates the results of a series of studies on the supply of O2 and substrates for oxidative muscle metabolism and draws conclusions on the role of structural design in partitioning and limiting substrate supply. The studies compared dogs and goats exercising at different intensities and combined physiological, biochemical and morphometric investigations. In both species, the rate of fatty acid oxidation reached an upper limit at low exercise intensities, and only glucose consumption was increased at higher exercise intensities. The supply of both glucose and fatty acids from the capillaries reached maximal rates at low exercise intensities; this limitation is related to the design of the sarcolemma as calculations suggest that the endothelium introduces only a small resistance to substrate flux. From these findings, it appears that the capillaries are designed to satisfy O2 supply up to maximal O2 demand. The increase in substrate supply to the mitochondria at higher exercise intensities is achieved by drawing on intracellular stores of glycogen and lipids. The size of these stores is larger in dogs than in goats, providing the athletic species with twice the fuel reserves. These findings are interpreted on the basis of a network model with fluxes partitioned between direct and indirect pathways and with some structures shared by more than one function. Whereas O2 is supplied through a direct pathway, the supply of both substrates is split temporally to allow, during exercise, immediate fuel supply to the mitochondria from intracellular stores; these are replaced from the vasculature, during periods of rest, to a size commensurate with high rates of combustion. Considering this complexity, we conclude that the results are compatible with the principle of symmorphosis applied to a network structure and that the adjustment of design to functional demand involves different structures for O2 and for substrates.
Vitamin E Administration Attenuates the Tri-Iodothyronine-Induced Modification of Heart Electrical Activity in The Rat
Authors: venditti Paola , Leo Teodoro De , Meo Sergio Di 
Published: April 2021

ABSTRACT
This work was designed to determine whether the thyroid-hormone-induced modifications of heart electrical activity are, at least in part, due to increased free radical production. For this study, 60-day-old euthyroid, hyperthyroid and hyperthyroid vitamin-E-treated rats were used. Hyperthyroidism, elicited by a 10 day treatment with tri-iodothyronine, induced an increase in lipid peroxidation without changing the level of antioxidants. Intraperitoneal vitamin administration to hyperthyroid rats led to a reduction in lipid peroxidation and a non-significant increase in antioxidant level.
The hyperthyroid state was also associated with an increase in heart rate measured in vivo and a decrease in the duration of the ventricular action potential recorded in vitro. Administration of vitamin E attenuated the thyroid-hormone-induced changes in heart rate and action potential duration, which were, however, significantly different from those of the control euthyroid rats. These results suggest that vitamin E protects hyperthyroid heart against lipid peroxidation by mechanisms that may be independent of the changes in antioxidant systems. Moreover, the reduction in the tri-iodothyronine effects on heart electrophysiological properties indicates that such effects are mediated, at least in part, through a membrane modification, probably related to increased lipid peroxidation, involving a free radical mechanism.
Effect of treatment planning system parameters on beam modulation complexity for treatment plans with single-layer multi-leaf collimator and dual-layer stacked multi-leaf collimator
Authors: Quintero Paulo , Cheng Yongqiang , Benoit David , Moore Craig , Beavis Andrew 
Published: April 2021

Objective:
High levels of beam modulation complexity (MC) and monitor units (MU) can compromise the plan deliverability of intensity-modulated radiotherapy treatments. Our study evaluates the effect of three treatment planning system (TPS) parameters on MC and MU using different multi-leaf collimator (MLC) architectures.

Methods:
192 volumetric modulated arc therapy plans were calculated using one virtual prostate phantom considering three main settings: (1) three TPS-parameters (Convergence; Aperture Shape Controller, ASC; and Dose Calculation Resolution, DCR) selected from Eclipse v15.6, (2) four levels of dose-sparing priority for organs at risk (OAR), and (3) two treatment units with same nominal conformity resolution and different MLC architectures (Halcyon-v2 dual-layer MLC, DL-MLC & TrueBeam single-layer MLC, SL-MLC). We use seven complexity metrics to evaluate the MC, including two new metrics for DL-MLC, assessed by their correlation with γ passing rate (GPR) analysis.

Results:
DL-MLC plans demonstrated lower dose-sparing values than SL-MLC plans (p&lt;0.05). TPS-parameters did not change significantly the complexity metrics for either MLC architectures. However, for SL-MLC, significant variations of MU, target volume dose-homogeneity, and dose spillage were associated with ASC and DCR (p&lt;0.05). MU were found to be correlated (highly or moderately) with all complexity metrics (p&lt;0.05) for both MLC plans. Additionally, our new complexity metrics presented a moderate correlation with GPR (r&lt;0.65). An important correlation was demonstrated between MC (plan deliverability) and dose-sparing priority level for DL-MLC.

Conclusions:
TPS-parameters selected do not change MC for DL-MLC architecture, but they might have a potential use to control the MU, PTV homogeneity or dose spillage for SL-MLC. Our new DL-MLC complexity metrics presented important information to be considered in future pre-treatment quality assurance programs. Finally, the prominent dependence between plan deliverability and priority applied to OAR dose sparing for DL-MLC needs to be analyzed and considered as an additional predictor of GPRs in further studies.

Advances in knowledge:
Dose-sparing priority might influence in modulation complexity of DL-MLC.
Isothermal drying characteristics and kinetics of human faecal sludges
Authors: Somorin Tosin , Getahun Samuel , Septien Santiago , Mabbet Ian , Kolios Athanasios , Buckley Chris 
Published: April 2021

Background: Drying is an important step for the thermochemical conversion of solid fuels, but it is energy-intensive for treating highly moist materials. Methods: To inform the thermal treatment of faecal sludge (FS), this study investigated the drying characteristics and kinetics of various faecal wastes using thermogravimetric analysis and isothermal heating conditions. Results: The findings show that FS from the anaerobic baffled reactor (ABR) and ventilated improved pit latrines (VIP) exhibit similar drying characteristics, with maximum drying rates at 0.04 mg/min during a constant rate period that is followed by a distinct falling rate period. On the contrary, fresh human faeces (HF) and FS from urine diversion dry toilets (UDDT) exhibited a falling rate period regime with no prior or intermittent constant rate periods. The absence of a constant rate period in these samples suggested limited amounts of unbound water that can be removed by dewatering and vice versa for VIP and ABR faecal sludges. The activation energies and effective moisture diffusivity for the sludges varied from 28 to 36 kJ/mol and 1.7·10-7 to 10·10-7 m2/s at 55°C and sludge thickness of 3mm. The Page model was consistent in modelling the different sludges across all temperatures. Conclusions: These results presented in this study can inform the design and development of innovative drying methods for FS treatment.
The Profitability of Revenue-Based Quotas Under Price Negotiation
Authors: Jindal Pranav , Newberry Peter 
Published: April 2021

We study how the presence of a monthly revenue-based quota impacts a retailer’s profits when prices are negotiated by a salesperson. Using transaction level data for refrigerators, we first provide reduced-form evidence that prices are impacted by the quota: the negotiated discounts are approximately 3.8% higher if the salesperson is 10% closer to reaching the quota in the final week of the month. Guided by this result, we specify and estimate a demand model that identifies the impact of the quota through two forces: the effort salespeople expend in order to sell the product and their bargaining position. Results indicate that, as salespeople get closer to reaching their quota, their effort increases regardless of the week, and their bargaining position weakens (i.e., they offer lower prices), but only in the final week of the month. We use these results to analyze the impact of the quota and find that, holding salespeoples’ total compensation fixed, eliminating quotas results in 8% lower profit for the retailer. This decrease stems primarily from the reduction in effort that outweighs any benefit from strengthening the salespeoples’ bargaining position. The change in profit is economically meaningful because eliminating both price negotiation (i.e., moving to fixed pricing) and the quota results in an up to 36% reduction in profit. This paper was accepted by Matthew Shum, marketing.
Electron dynamics in laser-driven atoms near the continuum threshold
Authors: Liu Mingqing , Xu Songpo , Hu Shilin , Becker Wilhelm , Quan Wei , Liu Xiaojun , Chen Jing 
Published: April 2021

Strong-field ionization and Rydberg-state excitation (RSE) near the continuum threshold exhibit two phenomena that have attracted a lot of recent attention: the low-energy structure (LES) just above and frustrated tunneling ionization just below the threshold. The former becomes apparent for longer laser wavelengths, while the latter has been especially investigated in the near infrared; both have been treated as separate phenomena so far. Here we present a unified perspective based on electron trajectories, which emphasizes the very important role of the electron-ion Coulomb interaction as expected in this energy region. Namely, those trajectories that generate the LES can also be recaptured into a Rydberg state. The coherent superposition of the contributions of such trajectories with different travel times (each generating one of the various LES peaks) causes an oscillation in the intensity dependence of the RSE yield, which is especially noticeable for longer wavelengths. The theory is illustrated by RSE experiments at 1800 nm, which agree very well with the theory with respect to position and period of the oscillation. The wavelength scaling of the RSE oscillation is also discussed. Our work establishes a solid relationship between processes below and above the threshold and sheds new light on atomic dynamics driven by intense laser fields in this critical energy region.