bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue
Authors: Chen Sian , Chen Chenbin , Hu Yuanbo , Zhu Ce , Luo Xiaozhi , Wang Lizhu , Wang Xiang , Sun Xiangwei , Chen Xiaodong , Xie Wangkai , Lou Han , Huang Xielin , Li Chao , Xu Jun , Xue Xiangyang , Shen Xian 
Published: February 2021

Gastric cancer (GC) is one of the most common malignancies with high mortality and substantial morbidity. Although the traditional treatment strategies for GC revolve around surgery, radiotherapy, and chemotherapy, none have been able to optimally treat most affected patients. To improve clinical outcomes and overcome potential GC resistance, we established a three-dimensional (3D) culturing platform that accurately predicts drug responses in a time- and cost-effective manner. We collected tumor tissues from patients following surgeries and cultured them for 3 days using our protocol. We first evaluated cell proliferation, viability, and apoptosis using the following markers: Ki67 and cleaved caspase 3 (Cas3). We demonstrated that cell viability was maintained for 72 h in culture and that the tumor microenvironments and vascular integrities of the tissues were intact throughout the culture period. We then administered chemotherapeutics to assess drug responses and found differential sensitivity across different patient-derived tissues, enabling us to determine individualized medication plans. Overall, our study validated this rapid, cost-effective, scalable, and reproducible protocol for GC tissue culture that can be employed for drug response assessments. Our 3D culture platform paves a new way for personalized medication in GC and other tumors and can greatly impact future oncological research.
A Novel DNA Methylation Signature as an Independent Prognostic Factor in Muscle-Invasive Bladder Cancer
Authors: Xu Zhijie , Gujar Hemant , Fu Guanghou , Ahmadi Hamed , Bhanvadia Sumeet , Weisenberger Daniel J. , Jin Baiye , Gill Parkash S. , Gill Inderbir , Daneshmand Siamak , Siegmund Kimberly D. , Liang Gangning 
Published: February 2021

BackgroundMuscle-invasive bladder cancer (MIBC) accounts for approximately 20% of all urothelial bladder carcinomas (UBC) at time of diagnosis, and up to 30% of patients with non-muscle invasive UBC will progress to MIBC over time. An increasing body of evidence has revealed a strong correlation between aberrant DNA methylation and tumorigenesis in MIBC.ResultsUsing The Cancer Genome Atlas (TCGA) molecular data for 413 patients, we described a DNA methylation-based signature as a prognostic factor for overall survival (OS) in MIBC patients. By using a least absolute shrinkage and selection operator (LASSO) model, differentially methylated regions were first identified using multiple criteria followed by survival and LASSO analyses to identify DNA methylation probes related to OS and build a classifier to stratify patients with MIBC. The prognostic value of the classifier, referred to as risk score (RS), was validated in a held-out testing set from the TCGA MIBC cohort. Finally, receiver operating characteristic (ROC) analysis was used to compare the prognostic accuracy of the models built with RS alone, RS plus clinicopathologic features, and clinicopathologic features alone. We found that our seven-probe classifier-based RS stratifies patients into high- and low-risk groups for overall survival (OS) in the testing set (n = 137) (AUC at 3 years, 0.65; AUC at 5 years, 0.65). In addition, RS significantly improved the prognostic model when it was combined with clinical information including age, smoking status, Tumor (T) stage, and Lymph node metastasis (N) stage.ConclusionsThe DNA methylation-based RS can be a useful tool to predict the accuracy of preoperative and/or post-cystectomy models of OS in MIBC patients.
Development and Validation of a Predictive Model of Pain Modulation Profile to Guide Chronic Pain Treatment: A Study Protocol
Authors: Vincenot Matthieu , Coulombe-Lévêque Alexia , Sean Monica , Camirand Lemyre Félix , Gendron Louis , Marchand Serge , Léonard Guillaume 
Published: February 2021

Introduction: Quantitative sensory testing is frequently used in research to assess endogenous pain modulation mechanisms, such as Temporal Summation (TS) and Conditioned Pain Modulation (CPM), reflecting excitatory and inhibitory mechanisms, respectively. Numerous studies found that a dysregulation of these mechanisms is associated with chronic pain conditions. In turn, such a patient's “profile” (increased TS and/or weakened CPM) could be used to recommend different pharmacological treatments. However, the procedure to evaluate these mechanisms is time-consuming and requires expensive equipment that is not available in the clinical setting. In this study, we aim to identify psychological, physiological and socio-demographic markers that could serve as proxies to allow healthcare professionals to identify these pain phenotypes in clinic, and consequently optimize pharmacological treatments.Method: We aim to recruit a healthy participant cohort (n = 360) and a chronic pain patient cohort (n = 108). Independent variables will include psychological questionnaires, pain measurements, physiological measures and sociodemographic characteristics. Dependent variables will include TS and CPM, which will be measured using quantitative sensory testing in a single session. We will evaluate one prediction model and two validation models (for healthy and chronic pain participants) using multiple regression analysis between TS/CPM and our independent variables. The significance thresholds will be set at p = 0.05, respectively.Perspectives: This study will allow us to develop a predictive model to compute the pain modulation profile of individual patients based on their biopsychosocial characteristics. The development of the predictive model is the first step toward the overarching goal of providing clinicians with a set of quick and cheap tests, easily applicable in clinical practice to orient pharmacological treatments.
Minimally-Invasive Surgical Approach to Congenital Dacryostenosis: Proposal for a New Protocol
Authors: Pensiero Stefano , Diplotti Laura , Visalli Gianluca , Ronfani Luca , Giangreco Manuela , Barbi Egidio 
Published: February 2021

Background:Congenital dacryostenosis is one of the most common ophthalmological disorders in infants, with a high spontaneous resolution rate. In patients unresponsive to conservative treatment, the first-line approach is lacrimal drainage system probing, thought there is no clear consensus on optimal timing of surgery. The optimal treatment of patients unresponsive to primary probing is also controversial.Objectives:The aim of this study is to assess the optimal timing of probing in children with congenital dacryostenosis. Other purposes are to evaluate the efficacy of repeated probing and dacryointubation in patients unresponsive to the initial surgery without evident lacrimal outflow dysgenesis, and to determine the epidemiology of these maldevelopments.Methods:A retrospective consecutive cohort study was conducted in 625 eyes of 457 patients aged 7–48 months who underwent surgery for dacryostenosis. Patients were divided into 4 cohorts according to the timing of surgery. Data were analyzed using Fisher's test.Results:The success rate of primary probing was high, without significant differences between cohorts. One-third of recurrences were related to maldevelopments, the other two-thirds were treated with a second probing or dacryointubation, with high success rates, that did not significantly differ between the procedures. All cases unresponsive to the second surgery were resolved with dacryointubation.Conclusions:Probing is highly effective and its outcome is not affected by timing of surgery. Nevertheless, we advocate for early intervention, in order to identify possible maldevelopments, which require more invasive management. In patients unresponsive to primary probing, without evident maldevelopments, repeated probing should still be considered as the first-line approach, since it's less invasive but similarly effective to dacryointubation.
Estimating COVID-19 Pneumonia Extent and Severity From Chest Computed Tomography
Authors: Carvalho Alysson Roncally Silva , Guimarães Alan , Garcia Thiego de Souza Oliveira , Madeira Werberich Gabriel , Ceotto Victor Fraga , Bozza Fernando Augusto , Rodrigues Rosana Souza , Pinto Joana Sofia F. , Schmitt Willian Rebouças , Zin Walter Araujo , França Manuela 
Published: February 2021

BackgroundCOVID-19 pneumonia extension is assessed by computed tomography (CT) with the ratio between the volume of abnormal pulmonary opacities (PO) and CT-estimated lung volume (CTLV). CT-estimated lung weight (CTLW) also correlates with pneumonia severity. However, both CTLV and CTLW depend on demographic and anthropometric variables.PurposesTo estimate the extent and severity of COVID-19 pneumonia adjusting the volume and weight of abnormal PO to the predicted CTLV (pCTLV) and CTLW (pCTLW), respectively, and to evaluate their possible association with clinical and radiological outcomes.MethodsChest CT from 103 COVID-19 and 86 healthy subjects were examined retrospectively. In controls, predictive equations for estimating pCTLV and pCTLW were assessed. COVID-19 pneumonia extent and severity were then defined as the ratio between the volume and the weight of abnormal PO expressed as a percentage of the pCTLV and pCTLW, respectively. A ROC analysis was used to test differential diagnosis ability of the proposed method in COVID-19 and controls. The degree of pneumonia extent and severity was assessed with Z-scores relative to the average volume and weight of PO in controls. Accordingly, COVID-19 patients were classified as with limited, moderate and diffuse pneumonia extent and as with mild, moderate and severe pneumonia severity.ResultsIn controls, CTLV could be predicted by sex and height (adjusted R2 = 0.57; P &lt; 0.001) while CTLW by age, sex, and height (adjusted R2 = 0.6; P &lt; 0.001). The cutoff of 20% (AUC = 0.91, 95%CI 0.88–0.93) for pneumonia extent and of 50% (AUC = 0.91, 95%CI 0.89–0.92) for pneumonia severity were obtained. Pneumonia extent were better correlated when expressed as a percentage of the pCTLV and pCTLW (r = 0.85, P &lt; 0.001), respectively. COVID-19 patients with diffuse and severe pneumonia at admission presented significantly higher CRP concentration, intra-hospital mortality, ICU stay and ventilatory support necessity, than those with moderate and limited/mild pneumonia. Moreover, pneumonia severity, but not extent, was positively and moderately correlated with age (r = 0.46) and CRP concentration (r = 0.44).ConclusionThe proposed estimation of COVID-19 pneumonia extent and severity might be useful for clinical and radiological patient stratification.
Interactions Between Glycogen Synthase Kinase-3β Gene Polymorphisms, Negative Life Events, and Susceptibility to Major Depressive Disorder in a Chinese Population
Authors: Yang Jiarun , Ke Siyuan , Qiao Zhengxue , Yang Xiuxian , Qiu Xiaohui , Song Xuejia , Zhao Erying , Zhou Jiawei , Zhao Mingzhe , Yang Yanjie , Fang Deyu , Cao Depin 
Published: February 2021

Background: Recent studies suggest that glycogen synthase kinase (GSK)-3β is involved in the development of major depressive disorder (MDD). The aim of this study was to investigate the interaction between GSK-3β polymorphism (rs6438552, rs334558, and rs2199503) and negative life events in the pathogenesis of major depressive disorder (MDD).Methods: DNA genotyping was performed on peripheral blood leukocytes in 550 patients with MDD and 552 age- and gender-matched controls. The frequency and severity of negative life events were assessed by the Life Events Scale (LES). A chi-square method was employed to assess the gene-environment interaction (G × E).Results: Differences in rs6438552, rs334558, and rs2199503 genotype distributions were observed between MDD patients and controls. Significant G × E interactions between allelic variation of rs6438552, rs334558, and rs2199503 and negative life events were observed. Individuals with negative life events and carrying genotypes of rs6438552 A+, rs334558 A+, and rs2199503G+ have increased the risk of depression.Conclusions: These results indicate that interactions between the GSK-3β rs6438552, rs334558, and rs2199503 polymorphisms and environment increases the risk of developing MDD.
Combined Associations of Serum Ferritin and Body Size Phenotypes With Cardiovascular Risk Profiles: A Chinese Population-Based Study
Authors: Zhou Bowen , Liu Siyue , Yuan Gang 
Published: February 2021

Background:Serum ferritin (SF) has been correlated with one or more metabolic syndrome features associated with an increased risk for cardiovascular disease (CVD). This study explored the associations between SF and CVD risk factors among different body size phenotypes that were based on metabolic status and body mass index (BMI) categories.Methods:A cross-sectional study was performed using a cohort of 7,549 Chinese adults from the China Health and Nutrition Survey. Participants did not exhibit acute inflammation, were not underweight and were stratified based on their metabolic status and BMI categories. The metabolically at-risk status was defined as having two or more criteria of the Adult Treatment Panel-III metabolic syndrome definition, excluding waist circumference.Results:Compared with individuals without high SF, subjects with high SF had an increased risk of diabetes in the metabolically at-risk normal-weight (MANW) and metabolically at-risk overweight/obesity (MAO) groups. The multivariate-adjusted odds ratios (ORs) were 1.52 [95% confidence interval (Cls): 1.02, 2.28] and 1.63 (95% Cls: 1.27, 2.09), respectively. Adjusted ORs for hyperuricemia from high SF in metabolically healthy normal-weight (MHNW), metabolically healthy overweight/obesity (MHO), MANW, and MAO phenotypes were 1.78 (95% Cls: 1.26, 2.53), 1.42 (95% Cls: 1.03, 1.95), 1.66 (95% Cls: 1.17, 2.36), and 1.42 (95% Cls: 1.17, 1.73), respectively. Similarly, positive correlations of high SF with triglycerides, non-high-density lipoprotein cholesterol, and apolipoprotein B100 were observed in all phenotypes. No association between high SF and elevated low-density lipoprotein cholesterol were observed among participants who were metabolically at-risk, regardless of their BMI categories. However, the ORs for elevated low-density lipoprotein cholesterol from high SF were 1.64 (95% Cls: 1.29, 2.08) in the MHNW group and 1.52 (95% Cls:1.22, 1.91) in the MHO group, significantly. This study demonstrated that the highest ORs were in MAO with a high SF group for all unfavorable CVD risk factors except low-density lipoprotein cholesterol (allp&lt; 0.001).Conclusions:The associations of high SF with the prevalence of CVD risk factors, including diabetes, dyslipidemia, and hyperuricemia, vary in individuals among different body size phenotypes. In the MAO group, subjects with high SF levels exhibited worse CVD risk profiles than individuals without high SF.
Development of a Balance Recovery Performance Measure for Gait Perturbation Training Based on the Center of Pressure
Authors: Rieger Markus M. , Papegaaij Selma , Steenbrink Frans , Pijnappels Mirjam , van Dieën Jaap H. 
Published: February 2021

Background: The availability of instrumented treadmills that can apply unexpected perturbations during walking has made gait perturbation training more popular in clinical practice. To quantify and monitor balance recovery while training, easy to use measures are needed and may be based on integrated force plate data. Therefore, we aimed to quantify and evaluate different implementations of the recovery performance measure based on center of pressure data.Methods: Recovery performance was calculated based on differences in center of pressure trajectories between unperturbed walking and balance recovery after a perturbation. Five methodological choices leading to 36 different implementations were evaluated. Test-retest reliability, effect sizes, and concurrent validity were evaluated against trunk velocity measures.Results: Differences in measures of (dis-)similarity, time normalization and reference data affected reliability, sensitivity and validity and none of the performance measure implementations based on center of pressure trajectories was superior on all criteria. Measures assessing perturbation effects on trunk velocities provided more reliable and sensitive recovery outcomes.Discussion: Different implementations of the recovery performance measure can be chosen dependent on constraints imposed in the clinical setting.Conclusion: Quantifying recovery performance based on center of pressure data is possible and may be suitable to monitor improvement in recovery performance after gait perturbations in specific clinical setups. Validity of performance measures in general requires further attention.
Vocabulaire et mécanismes de la soumission dans les mythes du Kojiki. Le problème du koto-muke
Authors: Quiros Ignacio 
Published: February 2021

One possible way to summarize the guiding thread of the Kojiki’s myths could be to describe them as the narration of a “heavenly” lineage establishing an “earthly” realm. In the process of taking possession of that earth, the heavenly representatives, first deities, and then human emperors, had to face opposition from some wild deities and disobedient people. Victory went to the heavenly side, and the text depicts the overthrowing of the savage entities by means of a specific archaic term called koto-muke, which can be roughly translated as “submission,” and that is occasionally followed by the verb yahasu (“pacify”). Even though long historical research about this mysterious koto-muke precedes our work, we believe in the importance of thinking about this word anew in order to grasp the specific features of the notion of submission in the Kojiki. Indeed, as the narration leans heavily on this compound to epitomize the whole process of subjugation of the savages, one reasonably suspects the presence of some deeper layers of meaning below its surface. This is mainly due to the presence of the morpheme koto, which infuses its polysemous qualities into the compound koto-muke. Therefore, this work aims to redefine the archaic word koto-muke not only by means of a thorough narrative analysis but also by a meticulous philological approach to both the invariable word koto
and the verb muke.
Test operation of a FOPS®-S filter separator in the process of surface runoff purification from the car parking area
Authors: Греков М.А. , Елагин С.В. , Козинец Г.Л. , Леонов Л.В. , Чечевичкин А.В. , Якунин Л.А. 
Published: February 2021

Поверхностный сток с территорий крупных мегаполисов интенсивно загрязняется. Особую роль в загрязнении играют автопарковки, на территории которых формируются сточные воды с высокими концентрациями нефтепродуктов и взвешенных веществ. Целью работы была тестовая многосезонная эксплуатация фильтра-сепаратора ФОПС®-С при очистке поверхностного стока, поступающего в систему канализации с территории автопарковки. В ходе наблюдений установлено, что на протяжении 21 месяца испытаний фильтр ФОПС®-С обеспечивал эффективность очистки сильнозагрязненного поверхностного стока от взвешенных веществ 82–99% и нефтепродуктов 55–76% (при их максимальной концентрации в стоке 3000 и 10,5 мг/дм3 соответственно). Снижения эффективности очистки в течение 21 месяца не наблюдалось, в том числе в периоды отрицательных температур (два сезона). За время испытаний из фильтра ФОПС®-С дважды производилась гидровыгрузка задержанных загрязнений: взвешенных веществ (~ 130 кг) и нефтепродуктов (~ 2,1 дм3).
Surface runoff from large metropolitan areas is heavily polluted. Car parking areas are of overriding concern in pollution since the surface runoff generated on them contains high concentrations of oil products and suspended solids. The aim of the project was multi-season test operation of a FOPS®-S filter-separator for the purification of the surface runoff disposed into the sewer system from the parking area. In the course of observations, it was found that over 21 months of testing, the FOPS®-S filter provided for removing 82–99% suspended solids and 55–76% of oil products from heavily contaminated surface runoff (at their maximum concentration in the surface runoff 3000 and 10.5 mg/dm3, respectively). No decrease in the treatment efficiency was observed for 21 months, including the periods of negative temperatures (two seasons). During the tests the hydraulic discharge of the trapped pollutants from the FOPS®-S filter was carried out twice: suspended solids (~ 130 kg) and oil products (~ 2.1 dm3).
Architecture as the art of creating human-friendly places
Authors: Chęć-Małyszek Agnieszka 
Published: February 2021

The public space of a city plays a special role in the life of every human being, as it meets basic and at the same time most important needs related to safety and comfort of life. It is a combination of an idea and a technique, which for centuries has reflected the changes taking place in people's social and cultural life. While the city is a multi-layered structure with a clearly separated private and public zone, creating mutual relations between the buildings. Camillo Sitte saw the city urban spaces as a work of art, które should be designed in such a way that the inhabitants feel safe and happy, as it is not just a show-off of technical skill, but an artistic undertaking. [1] The art of designing architecture does not exist for itself, but is created for the target audience. It provides a harmony that satisfies human needs and guarantees survival. It is an important factor influencing the development of an individual through the organization of a social living space. Urban spaces are primarily people and their needs that change over time. The first part of the article is devoted to the role of public spaces and the idea of the city as a work of art. The second part, in turn, is an attempt to define architecture as a kind of fine arts, taking into account the role it plays in the social life of Lublin's residents. The article is an attempts to emphasize the importance of architecture in designing a human-friendly environment as an art design that meets social expectations with the use of selected examples urban space of the city of Lublin.
Urban design and therapeutic landscapes. Evolving theme.
Authors: Trojanowska Monika 
Published: February 2021

The global pandemic of COVID-19, which enforced strict isolation, demonstrated the responsibility of architects and planners for public health promotion. While the concept of therapeutic landscapes is rather new, the quest for designing health-promoting human environment is at least as old as urban planning. The garden suburbs of Ebenezer Howard and Patrick Geddes were planned to promote health and well-being. The ultimate goal of Le Corbusier was to provide daylight and fresh air to each apartment. However, towers in open green space, which were built according to his visions, turned into unfriendly large suburbs. Today, the definition of therapeutic landscapes encompasses not only physical aspects of environment but also social and psychological factors. This study focuses on attempts to create health-promoting places. The interesting question is what are the architectural features linked to health promotion? Although the individual perception of health-promoting places is subjective and may vary, there are some objective qualities of eco-neighbourhoods that are conducive to human health and longevity. This study combines literature review with field observation and resultes in the development of the conceptual framework which can be consolidated into the universal standards for health-promoting places.