bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phage display and other peptide display technologies
Authors: Jaroszewicz Weronika , Morcinek-Orłowska Joanna , Pierzynowska Karolina , Gaffke Lidia , Węgrzyn Grzegorz 
Published: October 2021

ABSTRACT
Phage display technology, which is based on the presentation of peptide sequences on the surface of bacteriophage virions, was developed over 30 years ago. Improvements in phage display systems have allowed us to employ this method in numerous fields of biotechnology, as diverse as immunological and biomedical applications, the formation of novel materials and many others. The importance of phage display platforms was recognized by awarding the Nobel Prize in 2018 ‘for the phage display of peptides and antibodies’. In contrast to many review articles concerning specific applications of phage display systems published in recent years, we present an overview of this technology, including a comparison of various display systems, their advantages and disadvantages, and examples of applications in various fields of science, medicine and the broad sense of biotechnology. Other peptide display technologies, which employ bacterial, yeast and mammalian cells, as well as eukaryotic viruses and cell-free systems, are also discussed. These powerful methods are still being developed and improved; thus, novel sophisticated tools based on phage display and other peptide display systems are constantly emerging, and new opportunities to solve various scientific, medical and technological problems can be expected to become available in the near future.
Experiencing and Forecasting COVID-19 Daily Stress on Mental Health Reactivity Across Age and Race
Authors: Pearman Ann , Hughes MacKenzie L , Coblenz Clara W , Smith Emily L , Neupert Shevaun D 
Published: October 2021

Abstract

Objectives
This study systematically evaluates age and race differences in mental health symptoms as they unfold microlongitudinally during the coronavirus disease 2019 (COVID-19) pandemic with a focus on within-person reactivity to forecasting and experiencing COVID-19 stress.

Method
A daily diary approach was used to examine predictors of daily anxiety and depressive symptoms among 526 adults (White [54%] and Black American [46%]) aged 21–79. A total of 3,605 online diaries were collected for 21 consecutive days between October and November, 2020. In addition to mental health symptoms, participants reported forecasted (next 24 h) stress as well as experienced (past 24 h) stress related to COVID-19.

Results
Patterns of reactivity to forecasted and experienced COVID-19 stress depended on age and race. White older adults displayed greater reactivity to COVID-19-related stress than White younger adults, but the effects of COVID-19-related stress were consistently detrimental for the daily anxiety of Black Americans, regardless of age. For Black Americans, age was less negatively associated with depressive symptoms than for White Americans. Increases in experienced COVID-19 stress were also more strongly associated with increases in depressive symptoms for Black Americans relative to White participants.

Discussion
This study moves beyond cross-sectional, descriptive work within the context of the COVID-19 pandemic and emphasizes the dynamic nature of within-person reactivity patterns that differ by age and race. Although White older adults experienced an increase in daily anxiety when forecasting COVID-19 stress, the co-occurring pandemic of systemic racism may be more powerful than age-related vulnerabilities for Black adults.
Autoridad y disenso en la Corte Interamericana de Derechos HumanosAuthority and dissent in the Inter-American Court of Human Rights
Authors: Contesse Jorge 
Published: October 2021

Abstract
Legal scholars have paid significant attention to backlash and resistance against international tribunals. In general, the phenomenon is examined as coming from the outside, that is, from states dissatisfied with certain judgments. Resistance, however, may also come “from within,” when members of the tribunal themselves not only have interpretive disagreements, but challenge the way in which the Court shapes its authority. In this short work, I briefly examine two areas in which this interaction occurs and connect it to the more recent context of resistance against the Inter-American human rights system in order to begin a discussion about how the Court’s expansive interpretations may expand its jurisprudence, but ultimately at the cost of exposing the Court’s authority.
The Effect of Gain-versus-Loss Framing of Economic and Health Prospects of Different COVID-19 Interventions: An Experiment Integrating Equivalence and Emphasis Framing
Authors: Hameleers Michael , Boukes Mark 
Published: October 2021

AbstractA survey experiment was conducted that exposed Dutch citizens to different scenarios that either emphasized the gains or the losses regarding the number of victims or the economic damage caused by SARS-CoV-2. Replicating prospect theory in an ecologically valid crisis context, we found that gain frames promoted risk-aversive preferences, whereas loss frames increased support for risk-seeking alternatives. We further demonstrate the effect’s conditionality: Framing effects are strongest for health compared to economic scenarios and most pronounced when the type of intervention entails the highest risk associated with the respective domain. Theoretically, we show that the strongest media effects occur as an interplay between emphasis and equivalence framing effects, which underlines the need for media effects research to integrate both framing elements rather than studying them separately.
Almost Sure Large Fluctuations of Random Multiplicative Functions
Authors: Harper Adam J 
Published: October 2021

Abstract
We prove that if $f(n)$ is a Steinhaus or Rademacher random multiplicative function, there almost surely exist arbitrarily large values of $x$ for which $|\sum _{n \leq x} f(n)| \geq \sqrt{x} (\log \log x)^{1/4+o(1)}$. This is the first such bound that grows faster than $\sqrt{x}$, answering a question of Halász and proving a conjecture of Erd̋s. It is plausible that the exponent $1/4$ is sharp in this problem. The proofs work by establishing a multivariate Gaussian approximation for the sums $\sum _{n \leq x} f(n)$ at a sequence of $x$, conditional on the behaviour of $f(p)$ for all except the largest primes $p$. The most difficult aspect is showing that the conditional covariances of the sums are usually small, so the corresponding Gaussians are usually roughly independent. These covariances are related to a Euler product (or multiplicative chaos) type integral twisted by additive characters, which we study using various tools including mean value estimates for Dirichlet polynomials, high mixed moment estimates for random Euler products, and barrier arguments with random walks.
Public Debt Accumulation in SSA: A Looming Debt Crisis
Authors: Atingi-Ego Michael , Timuno Sayed , Makuve Tiviniton 
Published: October 2021

AbstractThis paper discusses recent debt trends and evaluates performance of debt sustainability analysis (DSA) conducted in a sample of Sub-Saharan African (SSA) countries over the period 2008–16. Based on qualitative and quantitative analyses, the findings suggest the existence of systematic optimism bias in past DSA vintages resulting from optimistic macro-economic projections that underpin the DSAs. As a result, the DSAs for the sample countries analysed projected higher debt carrying capacities, which in most cases led to a faster pace of debt accumulation during this period. Moreover, this was not helped by the fact that average interest rates on new debt commitments were rising faster relative to gross domestic product growth rates, while the necessary fiscal adjustment to counter this development remained insufficient. Countercyclical policies supported by fiscal buffers that were used to address the impact of the 2008 global financial crisis have largely not been reversed despite the erosion of the buffers and a pick-up in growth in some countries. As a result, the overall risk of debt distress in the region has deteriorated in the past decade. Going forward, strengthening analytical/research work on country macroeconomic projections underlying their DSAs, relationship between investment and growth, impact of natural disasters on DSA frameworks and quantifying and monitoring fiscal risks would be important pieces of work in leveraging DSA results for financing and policy decisions. Other supporting issues to embrace include capacity building to enhance quality of policies, transparency and accountability at institutional level; managing roll over risk; developing and implementing sound debt management strategies.
Outpatient portal use in prenatal care: differential use by race, risk, and area social determinants of health
Authors: Singh Priti , Jonnalagadda Pallavi , Morgan Evan , Fareed Naleef 
Published: October 2021

Abstract
To report the relationship of outpatient portal (OPP) use with clinical risk, area social determinants of health (SDoH), and race/ethnicity among pregnant women. Regression models predicting overall and individual portal feature use (main effects and interactions) based on key variables were specified using log files and clinical data. Overall OPP use among non-Hispanic Black women or patients who lived in lower SDoH neighborhoods were significantly less. High-risk pregnancy patients were likely to use the OPP more than those with normal-risk pregnancy. We found similar associations with individual OPP features, like Visit (scheduling) and My Record (test results). We also found significant interactive associations between race/ethnicity, clinical risk, and SDoH. Non-Hispanic Black women and those living in lower SDoH areas used OPP less than non-Hispanic White women from similar or affluent areas. More research must be conducted to learn of OPP use implications for pregnant women with specific clinical diagnoses.
Growth performance, visceral organ weights, and gut health of weaned pigs fed diets with different dietary fiber solubility and lipid sources
Authors: Hong Jinsu , Ndou Saymore Petros , Adams Seidu , Scaria Joy , Woyengo Tofuko Awori 
Published: October 2021

Abstract
The objective of this study was to determine the interactive effects of dietary fiber solubility and lipid source on growth performance, visceral organ weights, gut histology, and gut microbiota composition of weaned pigs. A total of 280 nursery pigs [initial body weight (BW) = 6.84 kg] weaned at 21 d were housed in 40 pens (7 pigs/pen). The pigs were fed four diets (10 pens/diet) in a randomized complete block design in two phases: Phase 1 from 0 to 2 wk and Phase 2 from 2 to 5 wk. The diets were corn-soybean meal-based with either sugar beet pulp (SBP) or soybean hulls (SBH) as a fiber source and either soybean oil (SBO) or choice white grease (CWG) as a lipid source in a 2 × 2 factorial arrangement. The BW and feed intake were determined by phase, whereas visceral organ weights, intestinal histology, and gut microbial composition were determined at the end of the trial. Dietary fiber solubility and lipid source did not interact (P &gt; 0.05) on average daily feed intake and average daily gain across all phases. However, the gain to feed ratio (G:F) for CWG-containing diets was lower (P &lt; 0.05) than that for SBO-containing diets for Phase 1. Also, G:F for SBP-containing diets was lower (P &lt; 0.05) than that for SBH-containing diets for Phase 1 and for the entire study period. Pigs fed SBP-containing diets had greater (P &lt; 0.05) stomach weight, and tended to have greater (P &lt; 0.10) small and large intestine weights relative to BW than those fed SBH-containing diets. Duodenal villous height to crypt depth ratio for CWG-based diets tended to be greater (P = 0.09) than that for SBO-based diets. Fiber solubility and lipid source interacted (P &lt; 0.05) on relative abundance of Bacteroides in the colon such that the relative abundance of the Bacteroides for CWG was greater (P &lt; 0.05) than that for the SBO in SBP-based diet, but not in SBH-based diet. Relative abundance of Butyricicoccus in the colon for SBH-based diet was greater (P &lt; 0.05) than that for SBP-based diet. In conclusion, inclusion of SBH instead of SBP in corn-soybean meal-based diets for weaned pigs can result in increased feed efficiency and relative abundance of Butyricicoccus in the colon, which is associated with improved gut health. Also, inclusion of SBO instead of CWG in the diets for weaned pigs can result in improved feed efficiency during Phase 1 feeding; however, the pigs may recover from the low feed efficiency induced by dietary inclusion of CWG instead of SBO after Phase 1 feeding.
Different Depths May Not Determine the Fluid Resuscitation Volume in Early-stage Management of Severe Burns: A Model-comparison Retrospective Analysis of Fluid Volume Determining Factors
Authors: Ning Fanggang , Jiang Huihao , Qiu Jiaming , Wang Lifang 
Published: October 2021

Abstract
Large-volume fluid resuscitation remains irreplaceable in the early-stage management of severe burns. We aimed to explore the relationship between fluid volume and other indicators. Data of severe burn patients with successful resuscitation in the early stage was collected. Correlation and linear regression analyses were performed. Multiple linear regression models, related goodness-of-fit assessment (adjusted R-square and Akaike Information Criterion), scatter plots, and paired t-test for two models, and a likelihood ratio test were performed. 96 patients were included. The median of total burn area (TBA) was 70%TBSA, with full thickness burn area (FTBA)/TBA of 0.4, a resuscitation volume of 1.93 mL/kg/%TBSA. Among volume-correlated indicators, two linear regression models were established (Model 1: TBA × weight and tracheotomy; and Model 2: FTBA × weight, partial thickness burn area (PTBA) × weight, and tracheotomy). For these models, close values of Akaike Information Criterion, adjusted R-squares, outliers of the prediction range, and the result of paired t-test, all suggest similarity between two models estimations, while the likelihood ratio test for coefficients of FTBA × weight and PTBA × weight showed a statistical difference. Inhalational injury and decompression surgery only correlated with volume, while Tracheotomy, TBA × weight, FTBA × weight, and PTBA × weight correlated with and were accepted in linear models of volume. Although FTBA and PTBA differed statistically, there may be no need to distinguish them when estimating the resuscitation volume requirements in this patient set. Further study about different depths fluid should be conducted.
Malnutrition in Burns: A Prospective, Single-Center Study
Authors: Caldis-Coutris Nancy , Gawaziuk Justin P , Magnusson Saul , Logsetty Sarvesh 
Published: October 2021

Abstract
The hypermetabolic response from a burn injury is the highest of the critically ill patient population. When coupled with the hypermetabolic response, preexisting malnutrition may increase the hospital resources used. The goal of this study was to evaluate the rate of malnutrition in burn patients and the associated hospital resource utilization. We collected prospective data on burn patients 18 years or older with a burn at least 10% TBSA admitted to a regional burn center. Demographics, %TBSA, comorbidities, length of stay (LOS), and standardized LOS (LOS/%TBSA) were evaluated on 49 patients. A multivariable regression model was constructed. Nutrition assessment was completed within 24 to 48 hours of admission including an SGA (Subjective Global Assessment) classification. SGA A (well-nourished) was compared to SGA B and C (malnourished). Fourteen patients (28.6%) in this study were malnourished. Malnourished patients were not statistically different with respect to median age (50 vs 39; P = .08) and body mass index (22.9 vs 26.5; P = .08) compared to the well-nourished group. However, malnourished patients had significantly longer median LOS (21.0 vs 11.0 days, P = .01) and LOS/%TBSA (1.69 vs 0.83, P = .001) than the well-nourished group. Being malnourished was a significant independent predictor of above-median LOS/%TBSA (P = .027) with an odds ratio of 5.61 (95% CI 1.215–25.890). The rate of malnutrition is important given the high metabolic demands of these patients. Malnutrition increased the resource requirements via higher standardized LOS. This underscores the importance of completing SGA on admission to identify malnutrition early on to optimize nutrition intervention during the patients’ hospital stay.
Health-Care Professionals and Lethal Injection: An Ethical Inquiry
Authors: Sawicki Sarah K 
Published: October 2021

Abstract
The practice of health-care professional involvement in capital punishment has come under scrutiny since the implementation of lethal injection as a method of execution, raising questions of the goals of medicine and the ethics of medicalized procedures. The American Medical Association and other professional associations have issued statements prohibiting physician involvement in capital punishment because medicine is dedicated to preserving life. I address the three primary arguments against health-care professionals being involved in lethal injection (healing, trust, and nonmaleficence) and argue that they are not strong enough to prohibit physician involvement in the lethal injection process.
An application of p-sulfonatocalix[6]arenes to attenuate cardiotoxicity of mitoxantrone in vitro: preparation, characterization and evaluation
Authors: Yu Xuan , Wang Meng , Wang Huimin , Ren Xiaoliang , Jiang Miaomiao , Zhu Yan , Zhang Deqin 
Published: October 2021

Abstract

Objectives
In this study, p-sulfonatocalix[6]arenes (SCA6) was proposed to construct a host–guest complexation to carry mitoxantrone (MIT) to maintain its anti-proliferation effect on HepG2 cells as well as to attenuate cardiotoxicity on H9C2 cells as a nano-size drug delivery system.

Methods
SCA6 binding to MIT evidenced through competitive fluorescence titration method. The complex was characterized using UV–visible, Fourier transform infrared, and proton nuclear magnetic resonance (1H-NMR) spectroscopies and differential scanning calorimetry analysis. The cytotoxicity was examined by a cell counting kit-8 assay on six cells. High content analysis, cell apoptosis and cell cycle experiments were measured to investigate the mechanism of detoxification in H9C2.

Key findings
The host–guest complexation was formed with a stoichiometry ratio of 1:1. Cytotoxicity study demonstrated that MIT/SCA6 complex could improve the cell viability on H9C2, MCF-7, A549, Hek293 and L02 cells and remained cytotoxicity effect on HepG2 cells. High content analysis showed that MIT/SCA6 complex could enhance the cell viability, mitochondrial mass and mitochondrial membrane potential and ameliorate the nuclear swelling on H9C2 cells. Moreover, the complex were arrested in G0/G1 phase of the cell cycle and same with MIT, while the detoxication was attributed to reducing early apoptosis.

Conclusions
The host–guest complexation between SCA6 and MIT had the ability to attenuate cardiotoxicity and provided a potential strategy for the application of soluble calixarenes in chemotherapy.
The effect of free and encapsulated cisplatin into long-circulating and pH-sensitive liposomes on IEC-6 cells during wound healing in the presence of host–microbiota
Authors: Araújo Raquel Silva , Cristina Oliveira Mônica , Cardoso Valbert Nascimento , Keefe Dorothy M K , Stringer Andrea M 
Published: October 2021

Abstract

Objectives
To circumvent cisplatin (CDDP) toxic effects and improve the antitumoural effect, our research group developed long-circulating and pH-sensitive liposomes containing CDDP (SpHL-CDDP). This study aimed to evaluate whether SpHL-CDDP is associated with intestinal protection under in-vitro conditions in the presence of host–microbiota, compared with free CDDP.

Methods
The cytotoxicity of CDDP and SpHL-CDDP were evaluated by colorimetric MTT and sulforhodamine B (SRB) assays. Epithelial proliferation was assessed by using an in-vitro wounding model in the presence of host–microbiota with intestinal epithelial cell line 6 (IEC-6) monolayers. Cytokines were determined by ELISA.

Key findings
Reduced cytotoxicity of SpHL-CDDP in IEC-6 cells (minimum of 1.3-fold according to the IC50 values) was observed when compared with CDDP. The presence of microbiota or CDDP reduced the wound healing. The association of microbiota and SpHL-CDDP improved the wound healing and cell number in IEC-6 cells when compared with control. These beneficial results can be associated with increased IL-6 and IL-10 levels induced by SpHL-CDDP which were affected by the presence of microbiota.

Conclusions
These results indicate that the presence of microbiota associated with SpHL-CDDP provided less intestinal cellular damages compared with CDDP and constitutes a promising candidate for clinical use.
An in-vitro comparative study of the binding of caspofungin and micafungin to plasma proteins
Authors: Yamasaki Keishi , Sakurama Keiki , Nishi Koji , Tsukigawa Kenji , Seo Hakaru , Otagiri Masaki , Taguchi Kazuaki 
Published: October 2021

Abstract

Objectives
Echinocandins are widely used for the treatment of invasive fungal diseases. While they bind strongly to plasma proteins, our knowledge of this process is not sufficient to permit their pharmacokinetics and pharmacodynamics targets to be discussed. In this study, we characterized the binding of two echinocandins, caspofungin and micafungin, to plasma proteins, human serum albumin (HSA) and human α 1-acid glycoprotein (AAG).

Methods
The binding parameters, number of binding sites (n) and association constant (K) for caspofungin and micafungin to HSA and AAG were determined by equilibrium dialysis. The binding site on HSA for these echinocandins was identified by conducting inhibition experiments.

Key findings
Caspofungin was found to bind strongly to a single site on HSA (n = 1.26, K = 0.45 × 106 M−1) and AAG (n = 0.99, K = 0.29 × 106 M−1). Micafungin was found to bind more strongly to HSA (n = 1.35, K = 1.44 × 106 M−1) and AAG (n = 1.32, K = 1.16 × 106 M−1). The binding site for these drugs on HSA appears to be within subdomain IA.

Conclusions
Free fraction of caspofungin and micafungin in patients may not be substantially affected due to the contribution of AAG to the overall protein binding and the binding to subdomain IA on HSA, which is different from the major drug-binding sites within subdomains IB, IIA and IIIA.
Contract Law in Practice
Authors: Andrews Neil 
Published: October 2021

Abstract
This book is a detailed examination of the general doctrines of English law of contract. Cases are analysed precisely, providing quick access to the major authoritative passages in the leading judgments. The coverage is comprehensive. It focuses on English law, but it also provides analysis of assistance throughout the Common Law family of legal systems. It provides up-to-date examination of case law developments. There are nearly fifty ‘evaluation’ sections which provide comment on controversial or unclear topics. Six major principles are identified: Freedom of Contract; Objectivity; the Contractual Bond Principle; Estoppel; Good Faith and Fair Dealing; the Compensation Principle.
Sources and General Features
Authors: Andrews Neil 
Published: October 2021

Abstract
This is an introductory chapter. The case law tradition of English contract law is explained, including the distinction between Common Law and Equity. The requirements of consensus are analysed. There is an overview of modern developments in the law. Doctrinal controversies are listed, with references to parts of the book where ‘Evaluation’ sections provide comment on difficult or uncertain developments or topics. There are forty-nine such ‘Evaluation’ sections, amongst which the author’s discussion of the following topics merits special attention: negotiation agreements [4.35] and [4.42]; the rule in Pinnel’s Case [6.82]; the ‘fiction of fraud’ and the Misrepresentation Act 1967 [11.113]; duress as to person [13.27]; illegitimate (but not unlawful) pressure [13.38]; good faith [19.27]; the White & Carter case [27.66].