bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acid Tolerant Bacterium Bacillus Amyloliquefaciens MBNC Retains Biocontrol Efficiency Against Fungal Phytopathogens in Low pH
Authors: Chowdhury Naimisha , Hazarika Dibya Jyoti , Goswami Gunajit , Sarmah Unmona , Borah Shrutirupa , Boro Robin Chandra , Barooah Madhumita 
Published: August 2021

Abstract
Soil pH conditions have important consequences for microbial community structure, their dynamics, ecosystem processes, and interactions with plants. Low soil pH affects the growth and functional activity of bacterial biocontrol agents which may experience a paradigm shift in their ability to act antagonistically against fungal phytopathogens. In this study, the antifungal activity of an acid tolerant soil bacterium Bacillus amyloliquefaciens MBNC was evaluated under low pH and compared to its activity in neutral pH conditions. Bacterial supernatant from three-day old culture grown in low pH conditions were more effective against fungal pathogens. B. amyloliquefaciens MBNC harboured genes involved in the synthesis of secondary metabolites of which surfactin homologues, with varying chain length (C11 – C15) were identified through High-Resolution Mass Spectroscopy. The pH of the medium influenced the production of these metabolites. Surfactin C15 was exclusive to the extract of pH 4.5; production of iturinA and surfactin C11 was detected only in pH 7.0 while, surfactin C12, C13 and C14 were detected in extracts of both the pH conditions. The secretion of phytohormones viz. Indole Acetic Acid (IAA) and Gibberellic Acid (GA) by B. amyloliquefaciens MBNC were detected in higher amount in neutral condition compared to acidic condition. Although, secretion of metabolites and phytohormones in B. amyloliquefaciens MBNC was influenced by the pH condition of the medium, the isolate retained its antagonistic efficiency against several fungal phyto-pathogens under acidic condition.
In Silico Protein- Protein Interaction of Pteoris Volitans Venom With Cancer Inducers of Helicobacter Pylori
Authors: Ravi Guru Nivetha , Unnikrishnan Sneha , Navabshan Irfan , Ramalingam Karthikeyan 
Published: August 2021

Abstract
Gastric cancer is a pathological condition induced by bacteria Helicobacter pylori. Targeting the key virulence factors of H.pylori causing gastric cancer is one such promising method for treating gastric cancer. Recently research has been focussed on analysing the adrenergeric, cholinergeric and anticancer properties of their venom proteins. Testing the anticancer activity of the lethal proteins in the venom of P.volitans not only provides a bioactive compound for cancer treatment but is also helpful to eliminate the ecological imbalance caused by this fish in marine environment. This study is focused on an in silico approach using Z-dock for analysing the bioactive prospective of the venom proteins of P.volitans against the key virulence proteins of H.pylori responsible for inducing cancer. Our in silico docking study using computational model of the venom proteins and H.pylori proteins has displayed the possible interactions between these proteins. The results revealed that the venom proteins of P.volitans hyaluronidase and PV toxin a effectively interacts with H.pylori proteins Cag A, Cag L, GGT, Cag D and Urease and may be promising proteins in cancer therapy.
The Role of Transfer Layer on the Sliding Wear Behavior of a Cu-15Ni-8Sn Alloy Under Different Loads
Authors: Li Daoxi , Wang Zhi , Zhao Chao , Luo Zongqiang , Zhang wengwen 
Published: August 2021

Abstract
We studied the microstructure of the transfer layer and its effect on the wear mechanism and wear property of an aged Cu-15Ni-8Sn alloy against GCr15 bearing steel during dry sliding by changing the applied load. The results indicate that the aged Cu-15Ni-8Sn alloy shows different wear behavior and wear properties when the applied load changed, where the average friction coefficient and specific wear rate decrease quickly with increasing applied load in steady wear condition. The sample tested under relatively high applied load shows the best wear performance owing the thickest oxide layer exists in the transfer layer. The main wear mechanisms were found changing with varied applied. The metallic nanocrystalline particles and the relative ductile copper oxides promotes the formation of a thick and densified oxide layer. The change of the thickness and morphology of the oxide layer under different load can significantly affect the wear mechanisms.
Social-Emotional Development of Children in Asia: A Systematic Review
Authors: Yong Geok Har , Lin Mei-Hua , Toh Teck Hock , Marsh Nigel V. 
Published: August 2021

Abstract
Background: There has been growing interest in the social-emotional development of children. However, the social-emotional development of children in Asia remains a knowledge gap. This systematic review identifies and summarises existing studies on the social-emotional development of children in Asia. Method: We conducted a systematic review using the Guidelines for Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA). This review included primary social-emotional development studies conducted in Asia and published in English. The study findings were entered into Microsoft Excel, and data were summarized quantitatively and thematically. Results: We reviewed 45 studies that met the inclusion criteria, and they were from 12 Asian countries, mostly the East Asia region (China and Hong Kong). Most of the studies were cross-sectional in design (n = 23, 51.1%). The majority of the studies focused on overall social-emotional development (n = 24, 53.3%), followed by social competence (n = 7, 15.6%), emotional development (n = 5, 11.1%), social-emotional learning (n = 3, 6.7%), problem behaviour (n = 3, 6.7%), self-regulation (n = 2, 4.4%), and 1 study (2.2%) focused on both social-emotional learning and problem behaviour. We did not perform the meta-analysis as the study findings were of heterogeneity.Conclusions: Studies on children’s social-emotional development in Asia are limited and mainly from the East Asia region. More diverse cultural studies on the social-emotional functioning of children in Asia are needed to understand children’s social-emotional development in Asia. Finally, parent and teacher knowledge on children’s social-emotional development should also be examined more closely. Systematic review registration: The protocol for this review was registered in PROSPERO (CRD42021238826).
Dendrimer Functionalized Metal Oxide Nanoparticle mediated Self-Assembled Collagen Scaffold for Skin Regenerative Application: Function of Metal in Metal oxides
Authors: Sree KJ , Vedhanayagam Mohan , Nair Balachandran Unni , kumar Anandasadagopan Suresh 
Published: August 2021

Abstract
Functionalized metal oxide nanoparticles cross-linked collagen scaffolds are widely used in skin regenerative applications because of their enhanced physico-chemical and biocompatibility properties. From the safety clinical trials point of view, there are no reports that have compared the effects of functionalized metal oxide nanoparticles mediated collagen scaffolds for in-vivo skin regenerative applications. In this work, Triethoxysilane - Poly (amido amine) dendrimer generation 3 (TES-PAMAM -G3 or G3) functionalized spherical shape metal oxide nanoparticles (MO NPs: ZnO, TiO2, Fe3O4, CeO2, and SiO2, Size: 12 -25 nm) cross-linked collagen scaffolds were prepared by using a self-assembly method. Triple helical conformation, pore size, mechanical strength and in-vitro cell viability of MO-TES-PAMAM-G3- collagen scaffolds were studied through different methods. The in-vivo skin regenerative proficiency of MO-TES-PAMAM-G3- collagen scaffolds were analysed by implanting the scaffold on wounds in Wistar Albino rats. The results demonstrated that MO-TES-PAMAM-G3- collagen scaffold showed superior skin regeneration properties than other scaffolds. The skin regenerative efficiency of MO NPs followed order: ZnO> TiO2> CeO2> SiO2> Fe3O4 NPs. This result can be attributed to higher mechanical strength, cell –viability and better antibacterial activity of ZnO-TES-PAMAM-G3-collagen scaffold lead to accelerate the skin regenerative properties in comparison to other metal oxide based collagen scaffolds.
Impact of COVID-19 infection on short-term outcome in patients referred to stress myocardial perfusion imaging
Authors: Assante Roberta , D’Antonio Adriana , Mannarino Teresa , Gaudieri Valeria , Zampella Emilia , Mainolfi Ciro , Cantoni Valeria , Green Roberta , Caiazzo Elisa , Nappi Carmela , Criscuolo Emanuele , Bologna Roberto , Zumbo Giulia , Petretta Mario , Cuocolo Alberto , Acampa Wanda 
Published: August 2021

Abstract
Purpose
We assessed the impact of COVID-19 infection on cardiovascular events in patients with suspected or known coronary artery disease (CAD) referred to stress myocardial perfusion single-photon emission computed tomography (MPS).
Methods
A total of 950 consecutive patients with suspected or known CAD were submitted by referring physicians to stress MPS for assessment of myocardial ischemia between January 2018 and June 2019. All patients underwent stress-optional rest MPS. Perfusion defects were quantitated as % of LV myocardium and expressed as total perfusion defect (TPD), representing the defect extent and severity. A TPD ≥ 5% was considered abnormal.
Results
During a mean follow-up of 27 months (range 4–38) 31 events occurred. Moreover, 55 (6%) patients had a COVID-19 infection. The median time from index MPS to COVID-19 infection was 16 months (range 6–24). At Cox multivariable analysis, abnormal MPS and COVID-19 infection resulted as independent predictors of events. There were no significant differences in annualized event rate in COVID-19 patients with or without abnormal MPS (p = 0.56). Differently, in patients without COVID-19 the presence of abnormal MPS was associated with higher event rate (p < .001). Patients with infection compared to those without had a higher event rate in the presence of both normal and abnormal TPD.
Conclusion
In patients with suspected and known CAD the presence of COVID-19 infection during a short-term follow-up was associated with a higher rate of cardiovascular events.
The TDP43 CFTS Affect Brain Endothelial Cell Functions by Regulating YAP and Tight Junction Proteins in Cerebral Ischemic Injury.
Authors: Xu Xiaotian , Zhang Changwen , Jiang Jianxiong , Xin Mei , Hao Jiukuan 
Published: August 2021

Abstract
Background: As important components of the blood-brain barrier (BBB), brain endothelial cells (ECs) interact with pericytes, astrocytes, neurons, microglia and extracellular matrix in the neurovascular unit to maintain central nerve system (CNS) homeostasis and regulate neurological functions. Pathological changes in brain endothelium plays an important role in progression of ischemic stroke. The compromised BBB under ischemic stroke condition causes neuronal damage. However, the pathophysiological mechanisms of BBB in normal and under ischemic stroke condition has not been fully elucidated.Methods: C57bl/6 mice were subjected to 1-hour transient middle cerebral occlusion (tMCAO) model, and collected the brain samples after reperfusion for 24hours, 72hours and 1week. Lentivirus (YAP/TAZ shRNA), adenovirus (YAPS112A), TDP43 siRNA, TDP43-CTFS35 overexpression plasmid, Oxygen-glucose deprivation (OGD), and lipopolysaccharides (LPS) were applied to brain endothelial cells in vitro experiments. Brain endothelial cells (ECs) functions were tested by cell proliferation, migration and cell viability. Hippo signaling pathway was examined by immunofluorescence and western blotting.Results: The present study demonstrates that TDP43 is an essential gene to regulate brain ECs normal function, and knockdown of TDP43 reduces tight junction protein expression and inhibits brain ECs migration. Furthermore, ischemic injury and inflammation induce cytoplasmic TDP43-CTFS35 aggregation brain ECs, which weaken TDP43 full-length’s function leading to impairing tight junction (TJ) protein expression and cell migration. The expression of cytoplasmic TDP43-CTFS35 in brain ECs increased at 24 hours and 72 hours after MCAO, but disappeared at 1 week after MCAO. The expressions of TJ proteins, ZO-1 and claudin-5, and expression of P-YAP are associated with the dynamic changes of TDP43-CTFS35 expression in brain ECs after MCAO. The underlying mechanism of TDP43-CTFS35’s effects on brain ECs is that TDP43-CTFS35 turns off the Hippo signaling pathway by inhibition of PMST1/2 phosphorylation leading to de-phosphorylate YAP, and subsequently causes brain ECs functional changes.Conclusions: The present study provides new insight knowledge regarding the mechanisms of brain vascular ECs regulation and pathological change in the BBB after cerebral ischemic injury.
Seasonal Ranging Pattern of Asian Elephants in the Protected Area Landscape of Bangladesh
Authors: Motaleb Mohammad Abdul , Ahmed Mohammad Sultan , Mahmud Md. Abdullah Al , Khan M. Monirul H. , Mamun Abdullah-Al , Huraira Abu , Rahman Md. Ashikur , Saha Samir , Chakma Sheeladitya , Hoque Mohammad Rashedul , Asma Roksana Akhter , Foisal Md. Zia Uddin , Rahman Md. Anisur , Arafat Mohammad Easin 
Published: August 2021

Abstract
Background

Seasonal ranging pattern of the Asian Elephants was assessed within two major Protected Areas, namely Chunati Wildlife Sanctuary and Teknaf Wildlife Sanctuary situated in the southern Bangladesh, for the period between November 2016 and May 2018. The objective of this study was to see how do the elephants move across the Protected Area landscapes, what was the linkage between the water sources and elephant’s seasonal movement patterns and how does vegetation cover link to elephant movement.
Methods

The ranging pattern was analyzed using the elephant dung distribution data. Normalized Difference Vegetation Index and Normalized Difference Water Index were estimated through satellite image analysis.
Results

Study clearly indicated that the ranging pattern of elephants fully depends on the vegetation and water sources. The areas where water sources and vegetation coverage were good, elephant movement was higher. The study identified few areas of these two Protected Areas where both vegetation coverage and water sources were visible and those areas as hot-spots for elephants.
Conclusions

The findings of this study can be used in conservation and management of elephants, particularly through the protection of preferred water sources and vegetation covered areas. In future these areas need to be protected and take proper management interventions.
Establishing Laboratory-Specific Reference Intervals for TSH and fT4 by use of the Indirect Hoffman Method
Authors: Płaczkowska Sylwia , Terpińska Małgorzata , Piwowar Agnieszka 
Published: August 2021

Abstract
Background: The results of examinations of laboratory parameters are the basis of appropriate medical decisions. The availability of reliable and accurate reference intervals (RIs) for each laboratory parameter is an integral part of its appropriate interpretation. Each medical laboratory should confirm their RIs. Up to date reference intervals for thyroid function hormones are still a matter of ongoing controversy. The aim of the study was application of the indirect Hoffman method to determine RIs for TSH and fT4 based on the large data pools stored in laboratory information systems, and the comparison of these RIs to generally used RIs.Material and methods: The TSH and fT4 routine examination results of a hospitalized and outpatients population over five years (2015-2019) were collected, and reference limits were established by the improved Hoffmann method after the exclusion of outliers. Comparative verification of established RIs was conducted with the RIs values provided by test manufacturers and literature data.Results: The various RIs in different age groups in examined populations were observed. For TSH RIs varied between different age groups, with a narrower range of RIs in the studied adult population, while for children, a shift of both reference boundaries toward higher values in comparison to manufacturers' data was observed. RIs estimated for fT4 were very similar to the manufacturer and literature data.Conclusion: Thyroid hormone levels change during a person's lifetime and vary between sexes, but this difference does not always influence the clinical interpretation of laboratory results in the context of RIs. The use of indirect methods is justified due to the ease and low cost of their application.
Selective Targeting of Angiopoietin-like 3 (ANGPTL3) with Vupanorsen for the Treatment of Patients with Familial Partial Lipodystrophy (FPLD): Results of a Proof-of-Concept Study
Authors: Foss-Freitas Maria Cristina , Akinci Baris , Neidert Adam , Bartlett Victoria J , Hurh Eunju , Karwatowska-Prokopczuk Ewa , Oral Elif 
Published: August 2021

Abstract
Background: Familial partial lipodystrophy (FPLD) is a rare disease characterized by selective loss of peripheral subcutaneous fat associated with dyslipidemia and diabetes mellitus. Reductions in circulating levels of ANGPTL3 are associated with lower triglyceride and other atherogenic lipids, making it an attractive target for treatment of FPLD patients. This proof-of-concept study was conducted to assess the efficacy and safety of targeting ANGPTL3 with vupanorsen in patients with FPLD. Methods: This was an open-label study. Four patients with FPLD (two with pathogenic variants in LMNA gene, and two with no causative genetic variant), diabetes (HbA1c≥7.0% and ≤12%), hypertriglyceridemia (≥500 mg/dL), and hepatic steatosis (hepatic fat fraction, HFF≥6.4%) were included. Patients received vupanorsen subcutaneously at a dose of 20 mg weekly for 26 weeks. The primary endpoint was the percent change from baseline in fasting triglycerides at Week 27. Other endpoints analyzed at the same time point included changes in ANGPTL3, fasting lipids and lipoproteins, insulin secretion/sensitivity, postprandial lipid and glycemic changes in response to a mixed meal test, HFF measured by MRI, and body composition measured by dual energy absorptiometry (DEXA). Results: Baseline mean ± SD fasting triglyceride level was 9.24 ± 4.9 mmol/L (817.8 ± 431.9 mg/dL). Treatment resulted in reduction in fasting levels of triglyceride by 59.9%, ANGPTL3 by 54.7%, and in several other lipoproteins/lipids including very low- density lipoprotein cholesterol by 53.5%, non-high-density lipoprotein cholesterol by 20.9%, and free fatty acids (FFA) by 41.7%. The area under the curve for postprandial triglycerides, FFA, and glucose were reduced by 60%, 32%, and 14%, respectively. Treatment with vupanorsen also resulted in 55% reduction in adipose tissue insulin resistance index, while other insulin sensitivity indices and HbA1c levels were not changed. Additional investigations in HFF and in DEXA parameters suggested dynamic changes in fat partitioning during treatment. Adverse events observed were related to common serious complications associated with diabetes and FPLD, vupanorsen was well tolerated, and there was no effect on platelet count. Conclusions: Although limited, these results suggest that targeting ANGPTL3 with vupanorsen could address several metabolic abnormalities in patients with FPLD.Clinical Trial: NCT03514420
Experimental research on the evaluation of left ventricular systolic function by layered speckle tracking before and after berberine treatment in a cardiac hypertrophy rat model
Authors: Luo haorou , Kou Tuli , Su Ye , Yin Lixue 
Published: August 2021

Abstract
Background

We aimed to detect the cardiac dysfunction in the early myocardial hypertrophy rat model by layered strain and to evaluate the effect of berberine on the left ventricular systolic function of myocardial hypertrophy rats.
Methods

80 healthy SD rats were randomly divided into 4 groups. The last three groups(ISO group) were injected with isoproterenol intraperitoneally to establish a model of myocardial hypertrophy;After successful modeling, group A and group B were given berberine hydrochloride intragastric administration (5mg/kg; 10mg/kg), and group C was given the same amount of normal saline by intragastric administration. The left ventricular systolic function was performed at the end of each trial using the layered strain technique. The myocardial tissue of rats was taken for pathological examination, and Masson trichrome staining and hematoxylin-eosin staining were performed. The myocardial interstitial fibrosis was evaluated and the correlation analysis was performed.
Results

The GCS, GSCendo and GSCmid of the ISO group were lower than those of the control group, and the difference was statistically significant (− 20.46% −29.57% −19.39% vs. −15.71% −20.11% −15.35%; P < 0.05). After berberine treatment, the GSCendo in group A (5mg/kg) and group B (10mg/kg) was higher than that in group C (− 24.49% vs.−26.87% vs.−20.09%; P < 0.001); The improvement in group B was more significant (− 24.49% vs.−26.87%; P < 0.001). Pathological results showed that, compared with the control group, fibrosis was significantly increased in the ISO group. After 7 days of berberine intragastric intervention, compared with group C without intervention, the fibrosis deposition in group A and B was reduced, and the fibrosis deposition in group B was more obvious. Spearman rank correlation analysis showed that Gscendo was positively correlated with the percentage of myocardial interstitial fiber deposition.
Conclusion

Persistent cardiac hypertrophy leads to marked myocardial fibrosis and necrosis. Berberine can inhibit the progression of myocardial fibrosis after the formation of cardiac hypertrophy. The stratified strain technique can identify early myocardial systolic dysfunction in patients with cardiac hypertrophy and evaluate the efficacy of short-term drug intervention, which is expected to be a new method for clinical quantitative evaluation of left ventricular systolic dysfunction.
Software-Defined-Radio Based HF Doppler Receiving System
Authors: Nakata Hiroyuki , Nozaki Kenro , Oki Yuhei , Hosokawa Keisuke , Hashimoto Kumiko , Kikuchi Takashi , Sakai Jun , Tomizawa Ichiro , Saita Satoko 
Published: August 2021

Abstract
High-frequency Doppler (HFD) sounding is a major remote sensing technique for monitoring the ionosphere. Conventional systems for HFDs mainly utilize analog circuits. However, existing analog systems have become difficult to maintain as the number of individuals adept at working with analog circuits has declined. To solve this problem, we developed an alternate HFD receiver system based on digital signal processing. The software-defined radio (SDR) technique enables the receiver to be set up without the knowledge of analog circuit devices. This approach also downsizes the system and reduces costs. A highly stabilized radio system for both the transmitter and receiver is necessary for stable long-term observations of various phenomena in the ionosphere. The global positioning system disciplined oscillator with an accuracy of H compensates for the frequency stability required by the new receiving system. In the new system, four frequencies are received and signal-processed simultaneously. The dynamic range of the new system is wider (> 130 dB) than that of the conventional system. The signal-to-noise ratio significantly improved by 20 dB. The new digital system enables radio waves to be received with much smaller amplitudes at four different frequencies. New digital receivers have been installed at some of the stations in the HFD observation network in Japan and have already captured various ionospheric phenomena, including medium-scale traveling ionospheric disturbances and sudden commencement induced electric field fluctuations, which indicates the feasibility of SDR for actual ionospheric observations. The new digital receiver is simple, inexpensive, and small in size, which makes it easy to deploy new receiving stations in Japan and elsewhere. These advantages of the new system will help drive the construction of a wide HFD observation network.