bluuebunny/crossref_metadata_2025_split huggingface dataset:
Job Transitions and Mental Health Outcomes Among U.S. Adults Aged 55 and Older During the COVID-19 Pandemic
Authors: Abrams Leah R , Finlay Jessica M , Kobayashi Lindsay C 
Published: April 2021

Abstract

Objectives
Adults around retirement age are especially vulnerable to the effects of the recent economic downturn associated with coronavirus disease 2019 (COVID-19). This study investigated disturbances to working life and mental health among Americans aged at least 55 during the early months of the pandemic.

Methods
Using data from the nationwide COVID-19 Coping Study (N = 6,264), we examined rates of job loss, furloughs, hour/income reductions, and work from home, along with unchanged work status, by age, gender, race/ethnicity, educational attainment, and occupation. We next described sources of worry by job transition group and tested the adjusted associations of COVID-19-related job transitions with life satisfaction, loneliness, depressive symptoms, and anxiety symptoms.

Results
Most job losses occurred among respondents younger than age 65 and those without college degrees. Job loss and reduced hours/income were more common among Hispanics compared to other racial/ethnic groups, and work from home transitions were most common among respondents with high educational attainment and jobs in government- and education-related occupations. Workers who lost their jobs had the lowest life satisfaction and the highest loneliness and depressive symptoms, followed by workers who were furloughed and workers with reduced hours/income. Work from home was associated with more anxiety than unchanged work.

Discussion
COVID-19-related job transitions are detrimental to mental health, even when they might keep workers safe. These results enhance our understanding of the potentially long-term mental health effects of social and economic aspects of the COVID-19 pandemic and highlight the need for economic and mental health support for aging Americans.
Sex Differences in the Association of Cigarette Craving With Insula Structure
Authors: Perez Diaz Maylen , Pochon Jean-Baptiste , Ghahremani Dara G , Dean Andy C , Faulkner Paul , Petersen Nicole , Tyndale Rachel F , Donis Andrea , Paez Diana , Cahuantzi Citlaly , Hellemann Gerhard S , London Edythe D 
Published: April 2021

Abstract

Background
Cigarette craving, which can negatively impact smoking cessation, is reportedly stronger in women than in men when they initiate abstinence from smoking. Identifying approaches to counteract craving in people of different sexes may facilitate the development of personalized treatments for Tobacco Use Disorder, which disproportionately affects women. Because cigarette craving is associated with nicotine dependence and structure of the insula, this study addressed whether a person’s sex influences these associations.

Methods
The research participants (n = 99, 48 women) reported daily cigarette smoking and provided self-reports of nicotine dependence. After overnight abstinence from smoking, they underwent structural magnetic resonance imaging scanning to determine cortical thickness of the left and right anterior circular insular sulcus, and self-rated their cigarette craving before and after their first cigarette of the day.

Results
Women reported stronger craving than men irrespective of smoking condition (i.e., pre- and post-smoking) (P = .048), and smoking reduced craving irrespective of sex (P &lt; .001). A 3-way interaction of sex, smoking condition, and right anterior circular insular sulcus thickness on craving (P = .033) reflected a negative association of cortical thickness with pre-smoking craving in women only (P = .012). No effects of cortical thickness in the left anterior circular insular sulcus were detected. Nicotine dependence was positively associated with craving (P &lt; .001) across groups and sessions, with no sex differences in this association.

Conclusions
A negative association of right anterior insula thickness with craving in women only suggests that this region may be a relevant therapeutic target for brain-based smoking cessation interventions in women.
Detection of Variants With Reduced Baloxavir Marboxil and Oseltamivir Susceptibility in Children With Influenza A During the 2019–2020 Influenza Season
Authors: Sato Masatoki , Takashita Emi , Katayose Masahiko , Nemoto Kenji , Sakai Nobuko , Fujisaki Seiichiro , Hashimoto Koichi , Hosoya Mitsuaki 
Published: April 2021

Abstract

Background
We aimed to detect influenza variants with reduced susceptibility to baloxavir marboxil (baloxavir) and oseltamivir and identify differences in the clinical course between children with and without these variants after antiviral treatment.

Methods
During the 2019–2020 influenza season, we enrolled children with confirmed influenza A (20 treated with baloxavir and 16 with oseltamivir). We analyzed patients’ sequential viral RNA loads and infectious virus titers, the drug susceptibilities of clinical isolates, and amino acid substitutions in the viral polymerase acidic protein subunits or neuraminidase. We assessed patients’ clinical information using questionnaires.

Results
All viral RNA loads and virus titers were significantly decreased after treatment, but we detected baloxavir-resistant and oseltamivir-resistant variants in 5 of 20 and 3 of 16 patients, respectively. The duration of fever was similar between patients with and without the variants, but infectious viral shedding lasted 3 days longer in patients with baloxavir-resistant variants. In addition, the duration to improvement of clinical symptoms was longer in these patients (75.0 vs 29.5 hours; P = .106).

Conclusions
After antiviral treatment, the emergence of baloxavir-resistant variants may affect the patients’ clinical course, but oseltamivir-resistant variants had no clinical impact.
1′-Acetoxychavicol acetate inhibits NLRP3-dependent inflammasome activation via mitochondrial ROS suppression
Authors: Sok Sophia P M , Ori Daisuke , Wada Ayana , Okude Haruna , Kawasaki Takumi , Momota Masatoshi , Nagoor Noor Hasima , Kawai Taro 
Published: April 2021

Abstract
The nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing (NLRP) 3 inflammasome is a multiprotein complex that triggers Caspase-1-mediated IL-1β production and pyroptosis, and its dysregulation is associated with the pathogenesis of inflammatory diseases. 1′-Acetoxychavicol acetate (ACA) is a natural compound in the rhizome of tropical ginger Alpinia species with anti-microbial, anti-allergic and anti-cancer properties. In this study, we found that ACA suppressed NLRP3 inflammasome activation in mouse bone marrow-derived macrophages and human THP-1 monocytes. ACA inhibited Caspase-1 activation and IL-1β production by NLRP3 agonists such as nigericin, monosodium urate (MSU) crystals, and ATP. Moreover, it suppressed oligomerization of the adapter molecule, apoptosis-associated speck-like protein containing a CARD (ASC), and Caspase-1-mediated cleavage of pyroptosis executor Gasdermin D. Mechanistically, ACA inhibited generation of mitochondrial reactive oxygen species (ROS) and prevented release of oxidized mitochondrial DNA, which trigger NLRP3 inflammasome activation. ACA also prevented NLRP3 inflammasome activation in vivo, as evidenced in the MSU crystal-induced peritonitis and dextran sodium sulfate-induced colitis mouse models accompanied by decreased Caspase-1 activation. Thus, ACA is a potent inhibitor of the NLRP3 inflammasome for prevention of NLRP3-associated inflammatory diseases.
Facebook’s exploitative and exclusionary abuses in the two-sided market for social networks and display advertising
Authors: Lodvdahl Gormsen Liza , Llanos Jose Tomas 
Published: April 2021

Abstract
The German Facebook case has directly addressed the contentious interplay between data protection and competition law for the first time. The Bundeskartellamt’s theory of harm, which directly linked privacy violations to the strengthening of Facebook’s market power, proved controversial: it elicited strong criticism from the appeals court, but then was partially endorsed by the Bundesgerichtshof (German Federal Supreme Court). This article shows that an enforcement action against Facebook under Article 102 TFEU need not be controversial. We present empirical evidence confirming that Facebook’s ‘envelopment by privacy policy tying’ strategy exploits consumers, as it causes clear consumer harm on the market for social networks in the form of lack of choice and degradation of quality. In turn, such consumer harm on the ‘free’ side leads to a weakening of the competitive market structure and foreclosure of competitors on the ‘paid’ market for display advertising and other adjacent markets. This strategy falls neatly within the scope of Article 102 TFEU, irrespective of whether it also violates EU data protection law. In addition, the enveloping by privacy policy tying forms part of an overall anti-competitive strategy over which the Commission can assert jurisdiction and prosecute as a single and continuous infringement.
Heat stress during late pregnancy and postpartum influences genetic parameter estimates for birth weight and weight gain in dual-purpose cattle offspring generations
Authors: Halli Kathrin , Brügemann Kerstin , Bohlouli Mehdi , Yin Tong , König Sven 
Published: April 2021

Abstract
Impact of direct heat stress (HS) on genetic parameter estimates, i.e., HS close to the trait recording date, was verified in several previous studies conducted in dairy and beef cattle populations. The aim of the present study was to analyze the impact of time-lagged HS at different recording periods during late pregnancy (a.p.) and postpartum (p.p.) on genetic parameter estimates for birth weight (BWT) and weight gain traits (200 d- and 365 d-weight gain (200dg, 365dg)) in offspring of the dual-purpose cattle breed “Rotes Höhenvieh” (RHV). Furthermore, we estimated genetic correlations within traits across time-lagged climatic indicators, in order to proof possible genotype by environment interactions (G×E). Trait recording included 5,434 observations for BWT, 3,679 observations for 200dg and 2,998 observations for 365dg. Time-lagged climatic descriptors were classes for the mean temperature humidity index (mTHI) and number of HS days (nHS) from the following periods: 7 d-period a.p. (BWT), 56 d-period a.p., and 56 d-period p.p. (200dg and 365dg). Genetic parameters were estimated via 2-trait animal models, i.e., defining the same trait in different climatic environments as different traits. Genetic variances and heritabilities for all traits increased with increasing mTHI- and nHS-classes for all recording periods, indicating pronounced genetic differentiation with regard to time-lagged in utero HS and HS directly after birth. Similarly, in low mTHI- and nHS-classes indicating cold stress, genetic variances, and heritabilities were larger than for temperate climates. Genetic correlations substantially smaller than 0.80 indicating G × E were observed when considering same traits from mTHI- and nHS-classes in greater distance. Estimated breeding values (EBV) of the 10 most influential sires with the largest number of offspring records fluctuated across mTHI- and nHS-classes. Correlations between sire EBV for same traits from distant climatic classes confirmed the genetic correlation estimates. Sires displaying stable EBV with climatic alterations were also identified. Selection of those sires might contribute to improved robustness in the RHV outdoor population genetically.
Species Composition, Parous Rate, and Infection Rate of Anopheles Mosquitoes (Diptera: Culicidae) in Bahir Dar City Administration, Northwest Ethiopia
Authors: Getaneh Abel , Yimer Mulat , Alemu Megbaru , Dejazmach Zelalem , Alehegn Michael , Tegegne Banchamlak 
Published: April 2021

Abstract
Anopheles mosquitoes are the main vectors of malaria. There is little information on the current entomological aspects of Anopheles mosquitoes in Amhara region of northwestern Ethiopia. Therefore, the aim of this study was to assess the prevailing species composition, parous rate, and infection rate of Anopheles mosquitoes in the Bahir Dar city administration. A community-based cross-sectional study was conducted from January through July 2020. For this, six Centers for Disease Control and Prevention light traps (three traps indoor and three traps outdoor) were used to collect adult female Anopheles mosquitoes. The species were morphologically identified, and the parous and infection rates were determined via dissection of ovaries and salivary gland, respectively. A total of 378 adult female Anopheles mosquitoes comprised of three species (Anopheles d’thali, Anopheles rhodesiensis, and Anopheles gambiae complex) were collected and identified at the study sites. Anopheles rhodesiensis was the predominant species accounting for 90% of all collections at the Zenzelima site, followed by An. gambiae complex (6.5%). In contrast, An. gambiae complex was the predominant species at the Tis Abay site, comprising 94% of captures. The overall parous and infection rates were 35 (62.5%) and 1 (2.9%), respectively.
A cross-sectional study of the quality of life of patients living with type 1 diabetes treated with insulin glargine and neutral protamine Hagedorn insulin and the implications
Authors: Almeida Paulo H R F , Godman Brian , de Lemos Lívia L P , Silva Thales B C , De Assis Acúrcio Francisco , Guerra‑Junior Augusto Afonso , De Araújo Vânia E , Almeida Alessandra M , Alvares-Teodoro Juliana 
Published: April 2021

Abstract

Objectives
The study aim was to identify key factors associated with the health-related quality of life (HRQOL) of patients with type 1 diabetes mellitus (T1DM) treated with neutral protamine Hagedorn (NPH) insulin or human insulin analog glargine (IGLA).

Methods
We conducted two cross-sectional studies in Minas Gerais State, Brazil. One with 401 patients treated with IGLA, and the other with 179 T1DM patients treated with NPH. HRQOL was measured by Euroqol (EQ-5D-3L).

Key findings
Most participants were male (51%), aged between 18 and 40 years (47%), non-black (58%) and from the highest economic strata (A1-B2) (74%). Participants perceived their health as good/very good (51%), had one to three medical consultations in the previous year (51%), were not hospitalized in the previous year (74%), did not report angina (96%), diabetic neuropathy (90%), hearing loss (94%) or kidney disease (89%). Non-severe hypoglycaemia episodes in the last 30 days were reported by 17% of participants.

Conclusions
Higher HRQOL was associated with younger age (18–40 years), good/very good health self-perception, having had up to three medical consultations in the last year, not being hospitalized in the last year, having none to three comorbidities, not reporting angina, diabetic neuropathy, hearing loss or kidney disease and having had episodes of non-severe hypoglycaemia. In addition, the findings of our study demonstrated inequalities in access to treatment, which will be the subject of future research projects.
Clinical Practice Guideline by the Pediatric Infectious Diseases Society and the Infectious Diseases Society of America: 2021 Guideline on Diagnosis and Management of Acute Hematogenous Osteomyelitis in Pediatrics
Authors: Woods Charles R , Bradley John S , Chatterjee Archana , Copley Lawson A , Robinson Joan , Kronman Matthew P , Arrieta Antonio , Fowler Sandra L , Harrison Christopher , Carrillo-Marquez Maria A , Arnold Sandra R , Eppes Stephen C , Stadler Laura P , Allen Coburn H , Mazur Lynnette J , Creech C Buddy , Shah Samir S , Zaoutis Theoklis , Feldman David S , Lavergne Valéry 
Published: April 2021

AbstractThis clinical practice guideline for the diagnosis and treatment of acute hematogenous osteomyelitis (AHO) in children was developed by a multidisciplinary panel representing Pediatric Infectious Diseases Society (PIDS) and the Infectious Diseases Society of America (IDSA). This guideline is intended for use by healthcare professionals who care for children with AHO, including specialists in pediatric infectious diseases, orthopedics, emergency care physicians, hospitalists, and any clinicians and healthcare providers caring for these patients. The panel’s recommendations for the diagnosis and treatment of AHO are based upon evidence derived from topic-specific systematic literature reviews. Summarized below are the recommendations for the diagnosis and treatment of AHO in children. The panel followed a systematic process used in the development of other IDSA and PIDS clinical practice guidelines, which included a standardized methodology for rating the certainty of the evidence and strength of recommendation using the GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach. A detailed description of background, methods, evidence summary and rationale that support each recommendation, and knowledge gaps can be found online in the full text.
Tracking Antimicrobial Resistance in Neisseria gonorrhoeae from the Molecular Level Using Endocervical Swabs
Authors: Oree G , Naicker M , Maise H C , Tinarwo P , Ramsuran V , Abbai N S 
Published: April 2021

Abstract

Objective
The global emergence of drug resistance in Neisseria gonorrhoeae has resulted in the use of a range of antibiotics and is now a public health concern because this pathogen may become untreatable in the future. This study aimed to detect antimicrobial-resistant determinants in N. gonorrhoeae directly from endocervical specimens.

Methods
Three hundred seven pregnant women were enrolled in this study. Endocervical swabs were collected from consenting women and used for the detection of N. gonorrhoeae. Molecular indicators associated with penicillin, tetracycline, ciprofloxacin, azithromycin, spectinomycin, cefixime, and ceftriaxone resistance were detected by polymerase chain reaction.

Results
Of the 307 women, 24 (7.8%) tested positive for N. gonorrhoeae. The tetM gene carried on the American-type plasmid was shown to be present in all the specimens. Approximately 87.5% of the specimens carried the penicillinase-producing African-type plasmid, and the gyrase A gene carrying the Ser-91 mutation was shown to be present in 37.5% of the specimens. Mutations associated with azithromycin, spectinomycin, cefixime, and ceftriaxone resistance were not detected in the study specimens.

Conclusion
The detection of resistance determinants without the need for culture may prove to be more feasible for future epidemiological investigations focused on tracking antimicrobial susceptibility patterns in N. gonorrhoeae.
Transcriptional control of parturition: insights from gene regulation studies in the myometrium
Authors: Khader Nawrah , Shchuka Virlana M , Shynlova Oksana , Mitchell Jennifer A 
Published: April 2021

AbstractThe onset of labour is a culmination of a series of highly coordinated and preparatory physiological events that take place throughout the gestational period. In order to produce the associated contractions needed for foetal delivery, smooth muscle cells in the muscular layer of the uterus (i.e. myometrium) undergo a transition from quiescent to contractile phenotypes. Here, we present the current understanding of the roles transcription factors play in critical labour-associated gene expression changes as part of the molecular mechanistic basis for this transition. Consideration is given to both transcription factors that have been well-studied in a myometrial context, i.e. activator protein 1, progesterone receptors, oestrogen receptors, and nuclear factor kappa B, as well as additional transcription factors whose gestational event-driving contributions have been demonstrated more recently. These transcription factors may form pregnancy- and labour-associated transcriptional regulatory networks in the myometrium to modulate the timing of labour onset. A more thorough understanding of the transcription factor-mediated, labour-promoting regulatory pathways holds promise for the development of new therapeutic treatments that can be used for the prevention of preterm labour in at-risk women.
Topologies of synthetic gene circuit for optimal fold change activation
Authors: Litovco Phyana , Barger Natalia , Li Ximing , Daniel Ramez 
Published: April 2021

Abstract
Computations widely exist in biological systems for functional regulations. Recently, incoherent feedforward loop and integral feedback controller have been implemented into Escherichia coli to achieve a robust adaptation. Here, we demonstrate that an indirect coherent feedforward loop and mutual inhibition designs can experimentally improve the fold change of promoters, by reducing the basal level while keeping the maximum activity high. We applied both designs to six different promoters in E. coli, starting with synthetic inducible promoters as a proof-of-principle. Then, we examined native promoters that are either functionally specific or systemically involved in complex pathways such as oxidative stress and SOS response. Both designs include a cascade having a repressor and a construct of either transcriptional interference or antisense transcription. In all six promoters, an improvement of up to ten times in the fold change activation was observed. Theoretically, our unitless models show that when regulation strength matches promoter basal level, an optimal fold change can be achieved. We expect that this methodology can be applied in various biological systems for biotechnology and therapeutic applications.
Long-term renal survival of paediatric patients with lupus nephritis
Authors: Demir Selcan , Gülhan Bora , Özen Seza , Çeleğen Kübra , Batu Ezgi Deniz , Taş Nesrin , Orhan Diclehan , Bilginer Yelda , Düzova Ali , Ozaltin Fatih , Topaloğlu Rezan 
Published: April 2021

AbstractBackgroundChildhood-onset systemic lupus erythematosus (SLE) is more severe than adult-onset disease, including more frequent kidney involvement. This study aimed to investigate baseline clinical features, treatment modalities and short- and long-term renal outcomes of paediatric patients with lupus nephritis (LN).Materials and methodsThis study enrolled 53 LN patients out of 102 childhood-onset SLE patients followed at Hacettepe University between 2000 and 2020. The demographic and clinical data were reviewed retrospectively from the medical charts and electronic records. All SLE patients with renal involvement underwent renal biopsy either at the time of diagnosis or during follow-up.ResultsThe median age at onset of SLE was 13.3 years [interquartile range (IQR) 10.4–15.8]. The median follow-up duration was 43.1 months (IQR 24.3–69.3). Of the 102 SLE patients, 53 (52%) had LN. The most frequent histopathological class was Class IV LN (54.7%), followed by Class III (22.6%). The proportion of patients who achieved either complete or partial remission was 77.3% and 73% at 6 and 12 months, respectively. In the overall LN cohort, 5- and 10-year renal survival rates were 92% and 85.7%, respectively. The remission rate at Month 6 was significantly higher in mycophenolate mofetil (MMF)- and cyclophosphamide (CYC)-treated groups than other combination therapies (P = 0.02). Although no difference was found between the CYC and MMF response rates (P = 0.57) in proliferative LN (Classes III and IV), the majority of Class IV patients (79%) received CYC as induction therapy. There was no difference between the response rates in any treatment regimens at Month 12 (P = 0.56). In the multivariate analysis, male gender, requiring dialysis at the time of LN diagnosis and failure to achieve remission at 6 and 12 months were found to be associated with poor renal outcome.ConclusionsOur study demonstrated that male gender, failure to achieve remission at 6 and 12 months and requiring dialysis at the time of diagnosis were the best predictors of poor renal outcome. Therefore appropriate and aggressive management of paediatric LN is essential to achieve and maintain remission.