bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional expression and localisation of HOPS/TMUB1 in mouse lens
Authors: Bartoli Daniela , Piobbico Danilo , Castelli Marilena , Pieroni Stefania , Scopetti Damiano , Ferracchiato Simona , Di-Iacovo Nicola , Cagini Carlo , Servillo Giuseppe , Della-Fazia Maria Agnese 
Published: February 2021

Abstract
Transparency represents the functional phenotype of eye lens. A number of defined steps including quiescence, proliferation, migration and cell differentiation culminates in cell elongation and organelle degradation, allowing the light to reach the retina. HOPS (Hepatocyte Odd Protein Shuttling)/TMUB1 (Trans Membrane Ubiquitin-like containing protein 1) is a nucleo-cytoplasmic shuttling protein, highly expressed both in vivo and in vitro proliferating systems, bearing a ubiquitin-like domain. The present study shows HOPS expression during the phases of lens cell proliferation and fiber differentiation, and its localisation in lens compartments. In lens, HOPS localises mainly in the nucleus of central epithelial cells. During mitosis, HOPS/TMUB1 shuttles to the cytoplasm and returns to the nucleus at the end of mitosis. The differentiating cells share distinct HOPS/TMUB1 localisation in transitional zone depending on the differentiation phases. HOPS/TMUB1 is observed in lens cortex and nucleus. Here, it is attached to fibers, having a structural function with crystallin proteins, probably acting in the ubiquitin–proteasome system.
KLHDC7B-DT aggravates pancreatic ductal adenocarcinoma development via inducing cross-talk between cancer cells and macrophages
Authors: Li Mu-xing , Wang Hang-yan , Yuan Chun-hui , Ma Zhao-lai , Jiang Bin , Li Lei , Zhang Li , Xiu Dian-rong 
Published: February 2021

Abstract
Tumor microenvironment (TME) exerts key roles in pancreatic ductal adenocarcinoma (PDAC) development. However, the factors regulating the cross-talk between PDAC cells and TME are largely unknown. In the present study, we identified a long noncoding RNA (lncRNA) KLHDC7B divergent transcript (KLHDC7B-DT), which was up-regulated in PDAC and correlated with poor survival of PDAC patients. Functional assays demonstrated that KLHDC7B-DT enhanced PDAC cell proliferation, migration, and invasion. Mechanistically, KLHDC7B-DT was found to directly bind IL-6 promoter, induce open chromatin structure at IL-6 promoter region, activate IL-6 transcription, and up-regulate IL-6 expression and secretion. The expression of KLHDC7B-DT was positively correlated with IL-6 in PDAC tissues. Via inducing IL-6 secretion, KLHDC7B-DT activated STAT3 signaling in PDAC cells in an autocrine manner. Furthermore, KLHDC7B-DT also activated STAT3 signaling in macrophages in a paracrine manner, which induced macrophage M2 polarization. KLHDC7B-DT overexpressed PDAC cells-primed macrophages promoted PDAC cell proliferation, migration, and invasion. Blocking IL-6/STAT3 signaling reversed the effects of KLHDC7B-DT on macrophage M2 polarization and PDAC cell proliferation, migration, and invasion. In conclusion, KLHDC7B-DT enhanced malignant behaviors of PDAC cells via IL-6-induced macrophage M2 polarization and IL-6-activated STAT3 signaling in PDAC cells. The cross-talk between PDAC cells and macrophages induced by KLHDC7B-DT represents potential therapeutic target for PDAC.
Optimization strategy of price‐based demand response considering the bidirectional feedback effect
Authors: Yang Hejun , Wang Lei , Ma Yinghao , Zhang Dabo , WU Hongbin 
Published: February 2021

AbstractTime of use pricing strategy can not only reduce the load fluctuation but also improve the power system reliability. Generally, before carrying out a time of use pricing optimization, it needs to perform a period partition optimization for dividing peak‐valley periods. In existing researches, however, the period partition optimization and the time of use pricing optimization are implemented independently. Although the output of period partition optimization is used as the input of time of use pricing optimization, the bidirectional feedback effect between period partition optimization and time of use pricing optimization is not considered, which will lead to the existing time of use pricing optimization may not be the globally optimal solution. Therefore, this paper investigates a new optimization method for time of use pricing optimization with the consideration of bidirectional feedback effect, establishes a comprehensive customer satisfaction degree model considering the customer load proportion coefficient, and defines an elastic cofactor to describe the electricity price response difference of multiple types of customers. The Roy Billinton test system is used to verify the correctness and effectiveness of the proposed method in this paper.
VVV survey near-infrared colour catalogue of known variable stars
Authors: Herpich F. R. , Ferreira Lopes C. E. , Saito R. K. , Minniti D. , Ederoclite A. , Ferreira T. S. , Catelan M. 
Published: February 2021

Context. The Vista Variables in the Via Lactea (VVV) near-infrared variability survey explores some of the most complex regions of the Milky Way bulge and disk in terms of high extinction and high crowding.
Aims. We add a new wavelength dimension to the optical information available at the American Association of Variable Star Observers International Variable Star Index (VSX-AAVSO) catalogue to test the VVV survey near-infrared photometry to better characterise these objects.
Methods. We cross-matched the VVV and the VSX-AAVSO catalogues along with Gaia Data Release 2 photometry and parallax.
Results. We present a catalogue that includes accurate individual coordinates, near-infrared magnitudes (ZYJHKs), extinctions AKs, and distances based on Gaia parallaxes. We also show the near-infrared CMDs and spatial distributions for the different VSX types of variable stars, including important distance indicators, such as RR Lyrae, Cepheids, and Miras. By analysing the photometric flags in our catalogue, we found that about 20% of the stars with measured and verified variability are flagged as non-stellar sources, even when they are outside of the saturation and/or noise regimes. Additionally, we pair-matched our sample with the VIVA catalogue and found that more than half of our sources are missing from the VVV variability list, mostly due to observations with low signal-to-noise ratio or photometric problems with a low percentage due to failures in the selection process.
Conclusions. Our results suggest that the current knowledge of the variability in the Galaxy is biased to nearby stars with low extinction. The present catalogue also provides the groundwork for characterising the results of future large variability surveys such as the Vera C. Rubin Observatory Legacy Survey of Space and Time in the highly crowded and reddened regions of the Galactic plane, as well as follow-up campaigns for characterising specific types of variables. The analysis of the incorrectly flagged stars can be used to improve the photometric classification of the VVV data, allowing us to expand the amount of data considered useful for science purposes. In addition, we provide a list of stars that are missed by the VIVA procedures for which the observations are good and which were missed due to some failure in the VIVA selection process.
Memories of past close encounters in extreme trans-Neptunian space: Finding unseen planets using pure random searches
Authors: de la Fuente Marcos C. , de la Fuente Marcos R. 
Published: February 2021

Context.The paths followed by the known extreme trans-Neptunian objects (ETNOs) effectively avoid direct gravitational perturbations from the four giant planets, yet their orbital eccentricities are in the range between 0.69−0.97. Solar system dynamics studies show that such high values of the eccentricity can be produced via close encounters or secular perturbations. In both cases, the presence of yet-to-be-discovered trans-Plutonian planets is required. Recent observational evidence cannot exclude the existence, at 600 AU from the Sun, of a planet of five Earth masses.Aims.If the high eccentricities of the known ETNOs are the result of relatively recent close encounters with putative planets, the mutual nodal distances of sizeable groups of ETNOs with their assumed perturber may still be small enough to be identifiable geometrically. In order to confirm or reject this possibility, we used Monte Carlo random search techniques.Methods.Two arbitrary orbits may lead to close encounters when their mutual nodal distance is sufficiently small. We generated billions of random planetary orbits with parameters within the relevant ranges and computed the mutual nodal distances with a set of randomly generated orbits with parameters consistent with those of the known ETNOs and their uncertainties. We monitored which planetary orbits had the maximum number of potential close encounters with synthetic ETNOs and we studied the resulting distributions.Results.We provide narrow ranges for the orbital parameters of putative planets that may have experienced orbit-changing encounters with known ETNOs. Some sections of the available orbital parameter space are strongly disfavored by our analysis.Conclusions.Our calculations suggest that more than one perturber is required if scattering is the main source of orbital modification for the known ETNOs. Perturbers might not be located farther than 600 AU and they have to follow moderately eccentric and inclined orbits to be capable of experiencing close encounters with multiple known ETNOs.
Dependency in a financialised global economy
Authors: Musthaq Fathimath 
Published: February 2021

ABSTRACT
Drawing on Samir Amin’s writings, this article proposes a contemporary form of dependency that manifests in the subordinate integration of developing countries into a financialised global economy. Using insights from the emergent financialisation literature, the article updates two themes in Amin’s work: imperialist rent and the role of the peripheral state in perpetuating dependency in the global economy. In contemporary capitalism, imperialist rent is not limited to labour arbitrage but also includes financial arbitrage, and the peripheral state, rather than retreating, now actively manages the financial sphere. The article advances an updated understanding of dependency in the context of financialisation.
Consideration of differences in drug usage between young-onset and elderly-onset rheumatoid arthritis with target of low disease activity
Authors: Kumagai Kosuke , Okumura Noriaki , Amano Yasutaka , Yayama Takafumi , Mimura Tomohiro , Maeda Tsutomu , Kubo Mitsuhiko , Mori Kanji , Barrett-Jolley Richard , Imai Shinji 
Published: February 2021

Abstract

Objectives
Elderly-onset rheumatoid arthritis (EORA) is reported to differ from young-onset rheumatoid arthritis (YORA) with regard to patient background and drug treatment. We examined the amount of drug administered to patients who achieved low disease activity (LDA) for rheumatoid arthritis at our hospital.

Methods
Demographics, clinical history, and treatments were compared between patients with EORA (n = 70) and YORA (n = 190).

Results
There was a significant difference in the average age (73.8 vs. 57.8 years), disease duration (6.66 vs. 14.7 years), and sex (62.9% males vs. 83.7% females), but no difference in rheumatoid factor positivity (85.3% vs. 80.7%), anti-citrullinated peptide antibody positivity (86.5% vs. 87.7%), simplified disease activity index (4.28 vs. 4.59), or disease activity score 28-CRP (1.99 vs. 2.04) in the EORA and YORA groups, respectively. There were also no significant differences in prednisolone use (37.1% vs. 36.3%), amount of methotrexate administered (MTX) (1.45 vs. 1.41 mg), and MTX use (55.7% vs. 65.3%). However, the MTX dose (2.89 vs. 4.09 mg/week, p = .011) and overall biologics use (32.9% vs. 56.3%, p = .0012) were significantly lower in patients with EORA than in those with YORA.

Conclusion
Patients with EORA may be able to achieve LDA with lower drug dosage than those with YORA.
Differences in knee joint degeneration between primary hip osteoarthritis and hip osteoarthritis secondary to hip developmental dysplasia: A propensity score-based analysis
Authors: Sato Ryuichi , Ando Wataru , Hamada Hidetoshi , Takao Masaki , Saito Mitsuru , Sugano Nobuhiko 
Published: February 2021

AbstractObjectivesThis study aimed to investigate differences in lower limb alignment and the prevalence of knee osteoarthritis (OA) among patients with primary hip osteoarthritis (PHOA) versus those with hip osteoarthritis secondary to developmental dysplasia of the hip (DDH-OA).MethodsWe compared 83 patients who underwent primary total hip arthroplasty for unilateral PHOA or DDH-OA after performing propensity score matching. The prevalence of knee OA and lower limb alignment were evaluated on preoperative plain radiographs.ResultsThe prevalence of knee OA on the ipsilateral side was significantly higher in the PHOA group than in the DDH-OA group (p =.019), whereas there was no difference between the groups on the contralateral side (p = .631). Lower-limb alignment was more valgus on the ipsilateral side in the DDH-OA group than the PHOA group, whereas it was not significantly different on the contralateral side between groups.ConclusionThe prevalence of knee OA and lower-limb malalignment on the ipsilateral side of hip OA were different for PHOA and DDH-OA patients. Shifting the mechanical axis of lower limbs might be associated with the prevalence of knee OA and lower limb malalignment in the presence of unilateral hip OA.
Application of comprehensive geophysical prospecting method in advance geological prediction of limestone tunnel
Authors: Chen Wu , Li Junjie , Zheng Huihua 
Published: February 2021

Abstract
Tunnel seismic prediction(TSP) and ground penetrating radar(GPR) are used in the advance geological prediction of a limestone tunnel. The working principle of two geophysical methods is briefly described. The additional detection work of GPR is carried out in the abnormal area of TSP prediction. The predicted dissolution distribution area of the two methods is relatively consistent. The actual excavation results of the tunnel verify the reliability of the geophysical prediction method. The results show that the velocity of TSP P-wave or S-wave corresponding to the dissolution zone is relatively low, which corresponds to the electromagnetic anomaly with strong amplitude of GPR with hyperbolic event.
Intraindividual Variability of Sleep Opportunity Attitudes, Perceived Norms, Perceived Behavioral Control, and Intentions
Authors: Mead Michael P , Irish Leah A 
Published: February 2021

AbstractBackgroundRecent research has used the theory of planned behavior (TPB) to predict sleep. However, this research has focused on between-person effects and has failed to capture the intraindividual variability of sleep-related attitudes, subjective norms, perceived behavioral control, and intentions.PurposeThe current study sought to characterize the between- and within-day patterns of these constructs.MethodsParticipants (N = 79) completed a 1 week ecological momentary assessment protocol in which they reported their attitudes, subjective norms, perceived behavioral control, and intentions toward nightly sleep opportunity four times per day.ResultsAnalyses revealed both between- and within-day variability of these constructs, with perceived behavioral control and intentions demonstrating greater variability than attitudes and subjective norms. Mixed linear models revealed that attitudes and subjective norms significantly increased throughout the week, while perceived behavioral control and intentions significantly decreased throughout the day.ConclusionsThe between- and within-day patterns of the TPB constructs highlight important methodological considerations and provide insight into the potential refinement of sleep promotion efforts.
Multicenter point prevalence evaluation of the utilization and safety of drug therapies for COVID-19 at the onset of the pandemic timeline in the United States
Authors: Rhodes Nathaniel J , Dairem Atheer , Moore William J , Shah Anooj , Postelnick Michael J , Badowski Melissa E , Michienzi Sarah M , Borkowski Jaime L , Polisetty Radhika S , Fong Karen , Spivak Emily S , Beardsley James R , Hale Cory M , Pallotta Andrea M , Srinivas Pavithra , Schulz Lucas T 
Published: February 2021

Abstract

Key points
In a multicenter point-prevalence study, we found that the rate of supportive care was high; among those receiving COVID-19 drug therapies, adverse reactions occurred in 12% of patients.

Purpose
There are currently no FDA-approved medications for the treatment of coronavirus disease 2019 (COVID-19). At the onset of the pandemic, off-label medication use was supported by limited or no clinical data. We sought to characterize experimental COVID-19 therapies and identify safety signals during this period.

Methods
We conducted a noninterventional, multicenter, point prevalence study of patients hospitalized with suspected/confirmed COVID-19. Clinical and treatment characteristics within a 24-hour window were evaluated in a random sample of up to 30 patients per site. The primary objective was to describe COVID-19–targeted therapies. The secondary objective was to describe adverse drug reactions (ADRs).

Results
A total of 352 patients treated for COVID-19 at 15 US hospitals From April 18 to May 8, 2020, were included in the study. Most patients were treated at academic medical centers (53.4%) or community hospitals (42.6%). Sixty-seven patients (19%) were receiving drug therapy in addition to supportive care. Drug therapies used included hydroxychloroquine (69%), remdesivir (10%), and interleukin-6 antagonists (9%). Five patients (7.5%) were receiving combination therapy. The rate of use of COVID-19–directed drug therapy was higher in patients with vs patients without a history of asthma (14.9% vs 7%, P = 0.037) and in patients enrolled in clinical trials (26.9% vs 3.2%, P &lt; 0.001). Among those receiving drug therapy, 8 patients (12%) experienced an ADR, and ADRs were recognized at a higher rate in patients enrolled in clinical trials (62.5% vs 22%; odds ratio, 5.9; P = 0.028).

Conclusion
While we observed high rates of supportive care for patients with COVID-19, we also found that ADRs were common among patients receiving drug therapy, including those enrolled in clinical trials. Comprehensive systems are needed to identify and mitigate ADRs associated with experimental COVID-19 treatments.
Acorus calamus extract and its component α-asarone attenuate murine hippocampal neuronal cell death induced by l-glutamate and tunicamycin
Authors: Mikami Masashi , Takuya Ohba , Yoshino Yuta , Nakamura Shinsuke , Ito Kenichi , Kojima Hiroyuki , Takahashi Tatsuji , Iddamalgoda Arunasiri , Inoue Shintaro , Shimazawa Masamitsu , Hara Hideaki 
Published: February 2021

ABSTRACT
The Asian traditional medicinal plant Acorus calamus and its component α-asarone exhibited various biological activities, such as antiinflammation and antioxidant effects. In the present study, we investigated the in vitro effects of A. calamus extract and α-asarone on oxidative stress- and endoplasmic reticulum (ER) stress–induced cell death in hippocampal HT22 cells. A. calamus extract and α-asarone both significantly suppressed cell death induced by the oxidative stress inducer l-glutamate and ER stress inducer tunicamycin. A. calamus extract and α-asarone also significantly reduced reactive oxygen species (ROS) production induced by l-glutamate. Moreover, A. calamus extract and α-asarone suppressed the phosphorylation of protein kinase RNA-like ER kinase (PERK) induced by tunicamycin. These results suggest that A. calamus extract and α-asarone protect hippocampal cells from oxidative stress and ER stress by decreasing ROS production and suppressing PERK signaling, respectively. α-Asarone has potential as a potent therapeutic candidate for neurodegenerative diseases, including Alzheimer's disease.
Skin-related enzyme inhibitory activity by hydrolyzable polyphenols in water chestnut (Trapa natans) husk
Authors: Yasuda Midori , Ikeoka Masaki , Kondo Shin-ichi 
Published: February 2021

ABSTRACTWater chestnut is a floating leaf plant native to Asia and Europe. Its fruit has long been used as an edible and herbal medicine. Water chestnut contains many polyphenols and its consumption can prevent lifestyle-related diseases because it has a suppressive effect on postprandial blood glucose elevation; however, its suitability as a cosmetic material is unknown. Therefore, this study aimed at investigating the antiaging effect of polyphenols contained in the husk of the devil water chestnut (Trapa natans). Six hydrolyzable polyphenols—1,6-di-O-galloyl-β-d-glucopyranose, 1,2,6-tri-O-galloyl-β-d-glucopyranose, 1,6-di-O-galloyl-2,3-O-(S)-hexahydroxydiphenoyl-β-d-glucopyranose (nobotanin D), eugeniin, 1,2,3,6-tetra-O-galloyl-β-d-glucopyranose, and trapain—were collected and isolated from the water chestnut husk. These polyphenols showed high antioxidant and antiglycation activities. In addition, inhibitory activities against hyaluronidase, elastase, and collagenase were observed. Especially, eugeniin and trapain, which have many gallic acids and a hexahydroxy-biphenyl group, showed high inhibitory activities. Thus, the polyphenols in water chestnut are beneficial for antiaging effects.
Quantitative analysis of auxin metabolites in lychee flowers
Authors: Kim Ryunhee , Osako Yutaro , Yamane Hisayo , Tao Ryutaro , Miyagawa Hisashi 
Published: February 2021

ABSTRACT
To investigate the modulation of endogenous indole-3-acetic acid (IAA) level by biosynthesis and inactivation during floral development, IAA and its metabolites were analyzed by LC-ESI/MS/MS in Lychee (Litchi chinensis Sonn.) flowers. In the bloomed flowers, the level of free IAA was higher in males than in females. In contrast, the total sum level of IAA metabolites was higher in females than in males, suggesting a higher biosynthetic activity of IAA in the females before the bloom. A detailed time-course analysis from the bud stage to the developing flower stage showed higher levels of IAA in females than males. The major metabolites were oxidized IAA in both sexes. The results suggest that IAA is involved in the maturation of female floral tissues in lychee, and oxidative metabolism plays an essential role in controlling the free IAA levels therein.
Thrombin-deficient mutant of medaka, a model fish, displays serious retardation in blood coagulation
Authors: Watanabe Yuko , Oguri Rina , Suzuki Risa , Meng Qi , Ishikawa Yuta , Tatsukawa Hideki , Hashimoto Hisashi , Hitomi Kiyotaka 
Published: February 2021

ABSTRACT
At the last stage of the blood coagulation cascade, thrombin plays a central role in the processing of fibrinogen for the polymerization and in the additional activation of Factor XIII for the stable cross-linking of fibrin. In addition, thrombin carries out possible multiple roles via processing or interaction with various functional proteins. Several studies conducted in order to elucidate additional physiological significance are ongoing. To clarify further significance of thrombin and to establish an associated disease model, we characterized the orthologue gene for medaka (Oryzias latipes), a research model fish. Tissue distribution of medaka prothrombin has been immunotechnically analyzed. Furthermore, thrombin-deficient medaka mutants were viably established by utilizing a genome-editing method. The established gene-deficient mutants exhibited retarded blood coagulation even in the heterozygous fish. Taking advantage of their ease of handling, this specific model is useful for further investigation in medical research areas on human coagulation diseases.