bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic review and meta-analysis of prognostic microRNA biomarkers for survival outcome in laryngeal squamous cell cancer
Authors: Huang Yan , Gu Min , Tang Yiting , Sun Zhiqiang , Luo Judong , Li Zhe 
Published: June 2021

Abstract
Background
Laryngeal carcinoma is a primary malignant tumor originating from the laryngeal mucosa, and its pathogenesis is not fully understood. It is a rare type of cancer that shows a downward trend in the 5-year survival rate. In clinical practice, dysregulated microRNAs are often observed in patients with laryngeal cancer. In recent years, an increasing number of studies have confirmed that the strong biomarker potential of microRNAs. We conducted a systematic review and meta-analysis to identify and highlight multiple microRNAs as biomarkers for disease prognosis in patients with laryngeal cancer.

Methods
We actively searched the systematic reviews in PubMed, Embase, Web of Science and The Cochrane Library to select the studies that met the proposed guidelines. A total of 5307 patients with laryngeal cancer were included in this study to evaluate the association between microRNAs expression levels and patient outcomes. For overall survival in the clinical stage, a hazard ratio (HR) and corresponding 95% confidence interval (CI) are calculated to assess the effect of survival.

Results
A total of 36 studies on microRNAs and laryngeal cancer recovery were included in this meta-analysis. The selected endpoints for these studies included overall survival (OS) and disease-free survival (DFS).The comorbidities of overexpression and underexpression of microRNAs were 1.13 (95% CI 1.06–1.20, P < 0.05) and 1.10 (95% CI 1.00–1.20, P < 0.05), respectively.

Conclusion
MiRNA-100, miRNA-155, miRNA-21, miRNA-34a, miRNA-195 and miR-let-7 are expected to be potential noninvasive and simple markers for laryngeal cancer.
Ex vivo and in vivo fluorescence detection and imaging of adenosine triphosphate
Authors: Chu Binbin , Wang Ajun , Cheng Liang , Chen Runzhi , Shi Huayi , Song Bin , Dong Fenglin , Wang Houyu , He Yao 
Published: June 2021

Abstract
Background
Ex vivo and in vivo detection and imaging of adenosine triphosphate (ATP) is critically important for the diagnosis and treatment of diseases, which still remains challenges up to present.

Results
We herein demonstrate that ATP could be fluorescently detected and imaged ex vivo and in vivo. In particular, we fabricate a kind of fluorescent ATP probes, which are made of titanium carbide (TC) nanosheets modified with the ROX-tagged ATP-aptamer (TC/Apt). In the constructed TC/Apt, TC shows superior quenching efficiency against ROX (e.g., ~ 97%). While in the presence of ATP, ROX-tagged aptamer is released from TC surface, leading to the recovery of fluorescence of ROX under the 545-nm excitation. Consequently, a wide dynamic range from 1 μM to 1.5 mM ATP and a high sensitivity with a limit of detection (LOD) down to 0.2 μM ATP can be readily achieved by the prepared TC/Apt. We further demonstrate that the as-prepared TC/Apt probe is feasible for accurate discrimination of ATP in different samples including living cells, body fluids (e.g., mouse serum, mouse urine and human serum) and mouse tumor models.

Conclusions
Fluorescence detection and imaging of ATP could be readily achieved in living cells, body fluids (e.g., urine and serum), as well as mouse tumor model through a new kind of fluorescent ATP nanoprobes, offering new powerful tools for the treatment of diseases related to abnormal fluctuation of ATP concentration.
Therapeutic analysis of Herbert screw fixation for capitellar fractures via the anterior approach in adolescent patients
Authors: Ju Lingpeng , Jiang Linjun , Zhang Yuan , Wu Jun , Li Ming , Liu Xing , Qu Xiangyang 
Published: June 2021

Abstract
Objective
The aim of this study is to analyze the efficacy of open reduction and Herbert screw fixation for coronal fractures of the capitellum via the anterior approach in adolescents.

Methods
We retrospectively analyzed the clinical and imaging data of 15 adolescents with capitellar fractures who were admitted to our hospital from May 2014 to May 2019. The fracture was reduced through the cubital crease incision via the anterior approach and was internally fixated with Herbert screws. A follow-up was conducted after the operation to examine fracture healing and elbow function. The postoperative functional recovery of patients was evaluated with the Mayo Elbow Performance index (MEPI) and the Broberg-Morrey rating system.

Results
Patients underwent surgery 3.7 days after injury on average. Intraoperative fracture reduction was satisfactory. No vascular injury or nerve injury occurred. Bony union occurred in an average of 6 weeks after the operation. All adolescents completed a 12- to 36-month follow-up. At the last follow-up, the Mayo Elbow Performance index was considered excellent in 12 patients and good in three patients. The Broberg-Morrey score was considered excellent in 12 patients, good in two patients, and fair in one patient.

Conclusion
Open reduction with Herbert screw fixation via the anterior approach is a feasible surgical method for the treatment of coronal fractures of the capitellum in adolescents.

Levels of evidence
Therapeutic, retrospective study-Level IV
Re-thinking treatment strategies for febrile neutropenia in paediatric oncology population: the perspective from a developing country
Authors: James Vinson , Prakash Anand , Mehta Kayur , Durugappa Tarangini 
Published: June 2021

Abstract
Background
This study was conducted to evaluate the microbiological profile of bacterial isolates in febrile neutropenia in a pediatric oncology unit, thereby, reviewing the use of restricted antibiotics and need for aggressive medical treatment accordingly.

Methods
A prospective observational study was conducted in a paediatric haemat-oncology division of a tertiary care teaching hospital in southern India from September 2014 to August 2016. One hundred and thirty children with febrile neutropenia were enrolled in the study. Blood cultures were performed using automated system. Cultures from other sites were obtained if needed, based on the clinical profile. Standard antibiotic susceptibility testing was done. Statistical analysis was done using SPSS.

Results
One hundred and thirty children were enrolled for the study. Two hundred and fifty episodes of febrile neutropenia were studied. Three hundred and eighty four cultures were sent and 92 (24%) cultures were positive. There were 48 (52.2%) Gram negative isolates followed by 33 (35.8%) Gram positive isolates, six (6.5%) fungal isolates and five (5.5%) poly-microbial cultures. Lactose fermenting Gram negative bacilli (20 isolates, 31.5%) were the most frequently isolated in the Gram negative group, with Escherichia coli being the most common organism (19 isolates, 20.6%). Amongst the Gram positive coagulase negative staphylococcus was the most common (twenty seven isolates, 29%). Escherichia coli and Non lactose fermenting gram negative bacteria (NFGNB) had only 36, 25% sensitivity to ceftazidime, respectively. Most Gram negative bacilli were found to have better sensitivity to amikacin (mean: 57%). There was a higher prevalence of extended spectrum beta lactamase producing organisms. Pan drug resistance, Extreme drug resistance and Multi drug resistance was found in three, twenty and thirteen Gram negative isolates respectively.Escherichia coli and Klebsiella were often drug resistant. Significantly higher mortality was associated with Gram negative isolates (eight deaths out of the thirteen deaths, 61.5%).

Conclusions
Our results show the importance of surveillance, monitoring resistance frequencies and identifying risk factors specific to each region. Given that significant mortality is attributed to drug resistant Gram negative bacilli, early initiation of appropriate antibiotics to cover for drug resistance is required while formulating empirical antibiotic policies for febrile neutropenia in the oncology units in the developing world.
Effect of Baduanjin exercise intervention on cognitive function and quality of life in women with breast cancer receiving chemotherapy: study protocol of a randomized controlled trial
Authors: Wei Xiao-Lin , Yuan Ru-Zhen , Jin Yong-Mei , Li Shu , Wang Ming-Yue , Jiang Jie-Ting , Wu Cai-Qin , Li Kun-Peng 
Published: June 2021

Abstract
Background
More than 50% cognitive impairment was reported by cancer patients before and after medical treatment. However, there are no effective interventions to manage the cognitive problem in women with breast cancer. This pilot study was designed to evaluate the protective effect of Baduanjin exercise on cognitive function and cancer-related symptoms in women with early-stage breast cancer undergoing chemotherapy.

Method
A single-blinded, randomized control trial was designed. The trial will recruit 70 patients with early-stage breast cancer scheduled to receive chemotherapy from Shanghai in China. All participants will be randomly assigned to (1:1) the supervised Baduanjin group (5 times/week, 30 min each time) or the wait-list control group for 3 months. The effect of Baduanjin exercise intervention will be evaluated by outcome measures including subjective and objective cognitive function, symptoms (fatigue, depression, and anxiety), and health-related quality of life at pre-intervention (T0), 8 weeks (T1), and 12 weeks (T2). The PCI score in the FACT-Cog as the primary cognitive outcome will be reported descriptively, while effect sizes and 95% confidence intervals (CIs) will be calculated. The collected data will be analyzed by using an intention-to-treat principle and linear mixed-effects modeling.

Discussion
This is the first randomized clinical trial to investigate whether Baduanjin exercise will have a positive role in improving cognitive function in women with breast cancer receiving chemotherapy. If possible, Baduanjin exercise will be a potential non-pharmacological intervention to manage cognitive dysfunction and promote survivorship care among breast cancer survivors.

Trial registration
Chinese Clinical Trial Registry (ChiCTR) ChiCTR2000033152. Registered on 22 May 2020
Joint Doppler shift and time delay estimation by deconvolution of generalized matched filter
Authors: Li Xuan , Ma Xiaochuan 
Published: June 2021

AbstractResolution probability is the most important indicator for signal parameter estimator, including estimating time delay, and joint Doppler shift and time delay. In order to get high-resolution probability, some procedures have been suggested such as compressed sensing. Based on the signal’s sparsity, compressed sensing has been used to estimate signal parameters in recent research. After solving ℓ0 norm Optimization problem, the methods would achieve high resolution. These methods all require high SNR. In order to improve the performance in low SNR, a novel implementation is proposed in this paper. We give a sparsity representation for the generalized matched filter output, or ambiguity function, while the former methods utilized the sparsity representation for channel response in time domain. By deconvolving the generalized matched filter output, 2-dimension estimation for Doppler shift and time delay would be gotten by greedy method, optimization method based on relaxation, or Bayesian method. Simulation demonstrates our method has better performance in low SNR than the method by the channel sparsity representation.
A novel fault section locating method based on distance matching degree in distribution network
Authors: Li Zhenxing , Wan Jialing , Wang Pengfei , Weng Hanli , Li Zhenhua 
Published: June 2021

AbstractFault section location of a single-phase grounding fault is affected by the neutral grounding mode of the system, transition resistance, and the blind zone. A fault section locating method based on an amplitude feature and an intelligent distance algorithm is proposed to eliminate the influence of the above factors. By analyzing and comparing the amplitude characteristics of the zero-sequence current transient components at both ends of the healthy section and the faulty section, a distance algorithm with strong abnormal data immune capability is introduced in this paper. The matching degree of the amplitude characteristics at both ends of the feeder section are used as the criterion and by comparing with the set threshold, the faulty section is effectively determined. Finally, simulations using Matlab/Simulink and PSCAD/EMTDC show that the proposed section locating method can locate the faulty section accurately, and is not affected by grounding mode, grounding resistance, or the blind zone.
Benchmark for scale-resolving simulation with curved walls: the Taylor Couette flow
Authors: Wang Z. J. , Jourdan E. 
Published: June 2021

AbstractFlow between rotating concentric cylinders, or the Taylor Couette flow, has been studied extensively because of its rich physics, ranging from axisymmetric steady laminar flow, to fully developed turbulent flow. In the present study, we advocate the use of this problem as a benchmark case for scale-resolving simulation, such as large eddy simulation (LES) and direct numerical simulation (DNS). The problem is attractive because of its simple geometry, simple boundary conditions, and complex physics involving wall-shear induced and centrifugal instability. Unlike the well-known fully developed channel flow, this problem has a curved wall boundary, and it is unnecessary to add a source term to the governing equations to sustain the fully developed turbulent flow. A p-refinement study for Re = 4000 is performed first to establish DNS data, including the time history of enstrophy, which can be used as an accuracy and resolution indicator to evaluate numerical methods, and is orders of magnitude faster than using the mean flow quantities and Reynolds stresses to evaluate solution quality. Finally, an hp-refinement study is performed to establish the relative accuracy and efficiency of high-order schemes of various accuracy.
Efficacy and Safety of Intravenous Pamidronate for Parathyroid Hormone-dependent Hypercalcemia in Hospitalized Patients
Authors: Rosenblum Rachel Chava , Twito Orit , Barzilay-Yoseph Liat , Ramaty Erez , Klein Noa , Rotman-Pikielny Pnina 
Published: June 2021

Abstract

Context
Bisphosphonates are effective for hypercalcemia of malignancy (HOM). Efficacy and safety data for bisphosphonates in parathyroid hormone–related hypercalcemia (PTHRH) are rare, including pamidronate (Pam), which is not indicated for this condition.

Objective
This work aims to evaluate the efficacy and safety of Pam for moderate-to-severe PTHRH.

Methods
This retrospective case-control study was conducted at a tertiary care medical center. Patients included adults hospitalized with serum calcium levels greater than 12 mg/dL, from October 29, 2013 to December 17, 2019. Etiology was categorized as PTHRH or PTH-independent. Clinical and laboratory data of PTHRH patients treated with Pam (PTHRH-Pam+) were compared to Pam-untreated counterparts (PTHRH-Pam–).

Results
Thirty-four patients with 37 hospitalizations for PTHRH (Pam-treated and -untreated) met the inclusion criteria. Pam was given in 24 of 37 cases (64.8%). Admission serum calcium levels for the PTHRH-Pam+ group were higher than for PTHRH-Pam– group (14.4 mg/dL vs 13.0 mg/dL, P = .005). Median total Pam dose was 60 mg (range, 30-180 mg) in the treated group. Serum calcium decreased 3.5 mg/dL for PTHRH-Pam+ vs 1.6 mg/dL for PTHRH-Pam– (P = .003). No PTHRH-Pam+ patients developed hypocalcemia or acute kidney injury. Nadir serum phosphorus levels were lower in the PTHRH-Pam+ vs PTHRH-Pam– group (1.7 mg/dL vs 2.4 mg/dL, respectively, P = .004). Three PTHRH-Pam+ patients developed severe hypophosphatemia; all resolved with intravenous and oral supplementation. Seventeen patients underwent parathyroidectomy, of whom 10 received Pam within 28 days preoperatively. Postoperatively, 4 developed hypocalcemia and 3 hypophosphatemia.

Conclusion
This study demonstrates that Pam is effective and safe for treating PTHRH, while ensuring close laboratory monitoring of calcium and phosphorus metabolism. Larger, prospective studies are needed to establish the role of Pam and other potent bisphosphonates in moderate-to-severe PTHRH.
Personalized Management of Pheochromocytoma and Paraganglioma
Authors: Nölting Svenja , Bechmann Nicole , Taieb David , Beuschlein Felix , Fassnacht Martin , Kroiss Matthias , Eisenhofer Graeme , Grossman Ashley , Pacak Karel 
Published: June 2021

AbstractPheochromocytomas/paragangliomas are characterized by a unique molecular landscape that allows their assignment to clusters based on underlying genetic alterations. With around 30% to 35% of Caucasian patients (a lower percentage in the Chinese population) showing germline mutations in susceptibility genes, pheochromocytomas/paragangliomas have the highest rate of heritability among all tumors. A further 35% to 40% of Caucasian patients (a higher percentage in the Chinese population) are affected by somatic driver mutations. Thus, around 70% of all patients with pheochromocytoma/paraganglioma can be assigned to 1 of 3 main molecular clusters with different phenotypes and clinical behavior. Krebs cycle/VHL/EPAS1-related cluster 1 tumors tend to a noradrenergic biochemical phenotype and require very close follow-up due to the risk of metastasis and recurrence. In contrast, kinase signaling–related cluster 2 tumors are characterized by an adrenergic phenotype and episodic symptoms, with generally a less aggressive course. The clinical correlates of patients with Wnt signaling–related cluster 3 tumors are currently poorly described, but aggressive behavior seems likely. In this review, we explore and explain why cluster-specific (personalized) management of pheochromocytoma/paraganglioma is essential to ascertain clinical behavior and prognosis, guide individual diagnostic procedures (biochemical interpretation, choice of the most sensitive imaging modalities), and provide personalized management and follow-up. Although cluster-specific therapy of inoperable/metastatic disease has not yet entered routine clinical practice, we suggest that informed personalized genetic-driven treatment should be implemented as a logical next step. This review amalgamates published guidelines and expert views within each cluster for a coherent individualized patient management plan.
Embodying Autonomous Trans Health Care in Zines
Authors: Raha Nat 
Published: June 2021

Abstract
This short article discusses recent trans health-care zines that have emerged from collectives rooted in radical care practices and mutual aid in the United Kingdom and Europe. This includes the publications Dysphoria, Power Makes us Sick, Radical Transfeminism, and Wages for Transition. It considers the embodied politics that emerge through the manifestos, writing, illustrations, and poems included within these zines, and the forms of bodily being they elaborate. In the context of the second half of a decade defined by fiscal austerity in Europe and the ongoing underresourcing of trans-specific health-care services in the United Kingdom, it details the practices and imaginaries of trans social reproduction, autonomy, and liberation that have emerged through these publications.
Altered germline cyst formation and oogenesis in Tex14 mutant mice
Authors: Ikami Kanako , Nuzhat Nafisa , Abbott Haley , Pandoy Ronald , Haky Lauren , Spradling Allan C. , Tanner Heather , Lei Lei 
Published: June 2021

ABSTRACT
During oocyte differentiation in mouse fetal ovaries, sister germ cells are connected by intercellular bridges, forming germline cysts. Within the cyst, primary oocytes form via gaining cytoplasm and organelles from sister germ cells through germ cell connectivity. To uncover the role of intercellular bridges in oocyte differentiation, we analyzed mutant female mice lacking testis-expressed 14 (TEX14), a protein involved in intercellular bridge formation and stabilization. In Tex14 homozygous mutant fetal ovaries, germ cells divide to form a reduced number of cysts in which germ cells remained connected via syncytia or fragmented cell membranes, rather than normal intercellular bridges. Compared with wild-type cysts, homozygous mutant cysts fragmented at a higher frequency and produced a greatly reduced number of primary oocytes with precocious cytoplasmic enrichment and enlarged volume. By contrast, Tex14 heterozygous mutant germline cysts were less fragmented and generate primary oocytes at a reduced size. Moreover, enlarged primary oocytes in homozygous mutants were used more efficiently to sustain folliculogenesis than undersized heterozygous mutant primary oocytes. Our observations directly link the nature of fetal germline cysts to oocyte differentiation and development.
High-throughput detection of eukaryotic parasites and arboviruses in mosquitoes
Authors: Cannon Matthew V. , Bogale Haikel N. , Bhalerao Devika , Keita Kalil , Camara Denka , Barry Yaya , Keita Moussa , Coulibaly Drissa , Kone Abdoulaye K. , Doumbo Ogobara K. , Thera Mahamadou A. , Plowe Christopher V. , Travassos Mark A. , Irish Seth R. , Yeroshefsky Joshua , Dorothy Jeannine , Prendergast Brian , St. Laurent Brandyce , Fritz Megan L. , Serre David 
Published: June 2021

ABSTRACT
Vector-borne pathogens cause many human infectious diseases and are responsible for high mortality and morbidity throughout the world. They can also cause livestock epidemics with dramatic social and economic consequences. Due to its high costs, vector-borne disease surveillance is often limited to current threats, and the investigation of emerging pathogens typically occurs after the reports of clinical cases. Here, we use high-throughput sequencing to detect and identify a wide range of parasites and viruses carried by mosquitoes from Cambodia, Guinea, Mali and the USA. We apply this approach to individual Anopheles mosquitoes as well as pools of mosquitoes captured in traps; and compare the outcomes of this assay when applied to DNA or RNA. We identified known human and animal pathogens and mosquito parasites belonging to a wide range of taxa, as well as DNA sequences from previously uncharacterized organisms. Our results also revealed that analysis of the content of an entire trap could be an efficient approach to monitor and identify rare vector-borne pathogens in large surveillance studies. Overall, we describe a high-throughput and easy-to-customize assay to screen for a wide range of pathogens and efficiently complement current vector-borne disease surveillance approaches.
S-acylation of Orai1 regulates store-operated Ca2+ entry
Authors: West Savannah J. , Kodakandla Goutham , Wang Qioachu , Tewari Ritika , Zhu Michael X. , Boehning Darren , Akimzhanov Askar M. 
Published: June 2021

ABSTRACT
Store-operated Ca2+ entry is a central component of intracellular Ca2+ signaling pathways. The Ca2+ release-activated channel (CRAC) mediates store-operated Ca2+ entry in many different cell types. The CRAC channel is composed of the plasma membrane (PM)-localized Orai1 channel and endoplasmic reticulum (ER)-localized STIM1 Ca2+ sensor. Upon ER Ca2+ store depletion, Orai1 and STIM1 form complexes at ER–PM junctions, leading to the formation of activated CRAC channels. Although the importance of CRAC channels is well described, the underlying mechanisms that regulate the recruitment of Orai1 to ER–PM junctions are not fully understood. Here, we describe the rapid and transient S-acylation of Orai1. Using biochemical approaches, we show that Orai1 is rapidly S-acylated at cysteine 143 upon ER Ca2+ store depletion. Importantly, S-acylation of cysteine 143 is required for Orai1-mediated Ca2+ entry and recruitment to STIM1 puncta. We conclude that store depletion-induced S-acylation of Orai1 is necessary for recruitment to ER–PM junctions, subsequent binding to STIM1 and channel activation.