bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly-potent, synthetic APOBEC3s restrict HIV-1 through deamination-independent mechanisms
Authors: McDonnell Mollie M. , Karvonen Suzanne C. , Gaba Amit , Flath Ben , Chelico Linda , Emerman Michael 
Published: April 2021

AbstractTheAPOBEC3(A3) genes encode cytidine deaminase proteins with potent antiviral and anti-retroelement activity. This locus is characterized by duplication, recombination, and deletion events that gave rise to the sevenA3s found in primates. These include three single deaminase domainA3s(A3A,A3C, andA3H) and four double deaminase domainA3s(A3B,A3D,A3F, andA3G). The most potent of the A3 proteins against HIV-1 is A3G. However, it is not clear if double deaminase domain A3s have a generalized functional advantage to restrict HIV-1. In order to test whether superior restriction factors could be created by genetically linking single A3 domains into synthetic double domains, we combined A3C and A3H single domains in novel combinations. We found that A3C/A3H double domains acquired enhanced antiviral activity that is at least as potent, if not better than, A3G. These synthetic double domain A3s have more efficiency of packaging into budding virions than their respective single domains, but this does not fully explain their gain of antiviral potency. The antiviral activity is conferred both by cytidine-deaminase dependent and independent mechanisms, with the latter correlating to an increase in RNA binding affinity. T cell lines expressing this A3C-A3H super restriction factor are able to control replicating HIV-1ΔVif infection to similar levels as A3G. Together, these data show that novel combinations of A3 domains are capable of gaining potent antiviral activity to levels similar to the most potent genome-encoded A3s, via a primarily non-catalytic mechanism.Author SummaryAntiviral genes are encoded by all organisms to help protect them from viral infections, including proteins encoded by primates to protect them from viruses similar to HIV-1. These antiviral proteins are also called “restriction factors”. Some restriction factors are broadly acting, while others are very specific. During the course of evolution, some of these genes have expanded into multiple copies and rearranged in different versions to give them new activities. However, not all versions of these genes have been sampled in nature. In this paper, we validated the hypothesis that one particular antiviral gene family, called theAPOBEC3family, has the capability of making novel combinations of antiviral genes with as great, or greater, potency against HIV-1 as the most potent natural member of this family. By combining parts of the APOBEC3 proteins into novel combinations, we created potent antiviral versions that act through a mechanism distinct from existing APOBEC3 proteins.
Limits to resilience of Afroalpine vegetation to grazing and burning: a case study of grasses from the Drakensberg Mountain Centre, southern Africa
Authors: Sylvester Steven P. , Soreng Robert J. , Mashau Aluoneswi C. , Sylvester Mitsy D.P.V. , Mapaura Anthony , Clark Vincent Ralph 
Published: April 2021

AbstractHigh-elevation Afroalpine ecosystems of the Drakensberg Mountain Centre (DMC) of Lesotho and South Africa, renowned for their high endemism and key ecosystem services, are socio-ecological systems that have seen human activity for millennia. However, their responses to land management practices are understudied. Controversy over their natural state has also led to conflicting policies and management emphases.Focusing on the crucial ecosystem-modulating component, grasses (Poaceae), we evaluate the response of DMC Afroalpine vegetation to human impact through grazing and burning. Grass species associations were recorded from grassland, shrubland and wetland-riparian-seep ecotypes across a range of grazing and fire regimes to document relationships between abiotic conditions, disturbance, and taxonomic diversity and composition.CCA of grass community composition retrieved a large cluster of plots of mixed grazing and burning regimes with no particular environmental vector correlated with them. Other smaller groups of plots separated from these were associated to heavy grazing, bioclimatic variables, slope gradient, and aspect. Indicator species analyses found DMC endemic grasses were associated to low grazing, while alien grasses were associated to heavy grazing. GLMs found little difference between ecotype-disturbance categories with regards plant species richness, mean alpha hull=2 range-size of native and sub-Saharan endemic grasses, and site-level Sørensen beta diversity (βsor). Some differences were noted, including the highest cover and proportion of DMC endemics being found in low-grazed grassland, and highest cover and proportion of alien grasses and highest plot-level βsor being found in heavily grazed ecotypes. Relative importance analyses found grazing regime to be the main influence on cover and proportion of DMC endemic and alien grasses. Partial Mantel tests found mean annual temperature and grazing regime to be the main influence on plot-level βsor.Synthesis: Taxonomic diversity and composition of DMC Afroalpine grasslands was relatively unaffected by moderate grazing and intense burning, although heavy grazing had a largely detrimental impact, with its ubiquity across the DMC a major cause for concern. High levels of endemism, coupled with the above data emphasizing the robustness of DMC grasslands to disturbance, also supports Afroalpine grasslands as a natural component of the DMC. This research reinforces the natural grass-dominated nature of the DMC as a social-ecological system where sustainable management is possible thanks to its resilience to grazing and burning, although current widespread overgrazing requires urgent attention.
TheDrosophilaTNF Eiger contributes to Myc super-competition independent of JNK activity
Authors: Kodra Albana L. , Singh Aditi Sharma , de la Cova Claire , Ziosi Marcello , Johnston Laura A. 
Published: April 2021

AbstractNumerous factors have been implicated in the cell-cell interactions that lead to elimination of cells via cell competition, a context-dependent process of cell selection in somatic tissues that is based on comparisons of cellular fitness. Here we use a series of genetic tests inDrosophilato explore the relative contribution of the pleiotropic cytokine Tumor Necrosis Factor ⍺ (TNF⍺) in Myc-mediated cell competition (also known as Myc super-competition or Myc cell competition). We find that the soleDrosophilaTNF, Eiger (Egr), its receptor Grindelwald (Grnd/TNFR), and the adaptor proteins Traf4 and Traf6 are required to eliminate wild-type “loser” cells during Myc cell competition. Although typically the interaction between Egr and Grnd leads to cell death by activating the Jun N-terminal Kinase (JNK) stress signaling pathway, our experiments reveal that many components of canonical JNK signaling are dispensable for cell death in Myc cell competition, including the JNKKK Tak1, the JNKK Hemipterous (Hep) and the JNK Basket (BSK). Our results suggest that Egr/Grnd signaling participates in Myc cell competition, but functions in a role that is independent of JNK activation.
Designing, optimizing, and assessing modular functional near-infrared brain imaging probes using an automated software workflow
Authors: Vanegas Morris , Mireles Miguel , Fang Qianqian 
Published: April 2021

AbstractSignificanceThe exponential growth of research utilizing functional near-infrared spectroscopy (fNIRS) systems has led to the emergence of modular fNIRS systems composed of repeating optical source/detector modules. Compared to conventional fNIRS systems, modular fNIRS systems are more compact and flexible, making wearable and long-time monitoring possible. However, the large number of design parameters makes designing a modular probe a daunting task.AimWe aim to create a systematic software platform to facilitate the design, characterization, and comparison of modular fNIRS probes.ApproachOur algorithm automatically tessellates any region-of-interest using user-specified module design parameters and outputs performance metrics such as spatial channel distributions, average brain sensitivity, and sampling rate estimates of the resulting probe. Automated algorithms for spatial coverage, orientation, and routing of repeated modules are also developed.ResultsWe developed a software platform to help explore a wide range of modular probe features and quantify their performances. We compare full-head probes using three different module shapes and highlight the trade-offs resulting from various module settings. Additionally, we show that one can apply this workflow to improve existing modular probes without needing to re-design or re-manufacture them.ConclusionOur flexible modular probe design platform shows promise in optimizing existing modular probes and investigating future modular designs.
Association of Shared Care Networks with Heart Failure Excessive Hospital Readmissions
Authors: Pinheiro Diego , Hartman Ryan , Mai Jing , Romero Erick , Soroya Saad , Bastos-Filho Carmelo , Lima Ricardo , Gibson Michael , Ebong Imo , Bidwell Julie T. , Nuño Miriam , Cadeiras Martin 
Published: April 2021

STRUCTURED ABSTRACTObjectivesThis study aimed to evaluate the impact of shared care networks on heart failure readmission rates.BackgroundHigher-than-expected heart failure (HF) readmissions affect half of US hospitals every year. The Hospital Reduction Readmission Program (HRRP) has reduced risk-adjusted readmissions, but it has also produced unintended consequences. Shared care models have been advocated for HF care, but the association of shared care networks with HF readmissions has never been investigated.MethodsWe curated publicly available data on hospital discharges and HF excessive readmission ratios (ERRs) from hospitals in California between 2012 and 2017. Shared Care Areas (SCAs) were delineated as data-driven units of care coordination emerging from discharge networks. The localization index (LI), the proportion of patients who reside in the same SCA in which they are admitted, were calculated by year. Generalized estimating equations (GEE) were used to evaluate the association between the LI and the ERR of hospitals controlling for race/ethnicity and socioeconomics factors.ResultsA total of 300 hospitals in California in a 6-yr period were included. The HF excessive readmission ratio (ERR) was negatively associated with the localization index (beta: -0.0474; 95% CI: -0.082 to -0.013). The percentage of Black residents within the SCAs was the only statistically significant covariate (beta: 0.4128; 95% CI: 0.302 to 0.524).ConclusionsHigher-than-expected HF readmissions were associated with shared care networks. Control mechanisms such as the HRRP may need to characterize and reward shared care to guide hospitals towards a more organized HF care system.
Cells of the human intestinal tract mapped across space and time
Authors: Elmentaite R , Kumasaka N , King HW , Roberts K , Dabrowska M , Pritchard S , Bolt L , Vieira SF , Mamanova L , Huang N , Goh Kai’En I , Stephenson E , Engelbert J , Botting RA , Fleming A , Dann E , Lisgo SN , Katan M , Leonard S , Oliver TRW , Hook CE , Nayak K , Perrone F , Campos LS , Dominguez-Conde C , Polanski K , Van Dongen S , Patel M , Morgan MD , Marioni JC , Bayraktar OA , Meyer KB , Zilbauer M , Uhlig H , Clatworthy MR , Mahbubani KT , Saeb Parsy K , Haniffa M , James KR , Teichmann SA 
Published: April 2021

AbstractThe cellular landscape of the human intestinal tract is dynamic throughout life, developingin uteroand changing in response to functional requirements and environmental exposures. To comprehensively map cell lineages in the healthy developing, pediatric and adult human gut from ten distinct anatomical regions, as well as draining lymph nodes, we used singlecell RNA-seq and VDJ analysis of roughly one third of a million cells. This reveals the presence of BEST4+ absorptive cells throughout the human intestinal tract, demonstrating the existence of this cell type beyond the colon for the first time. Furthermore, we implicate IgG sensing as a novel function of intestinal tuft cells, and link these cells to the pathogenesis of inflammatory bowel disease. We define novel glial and neuronal cell populations in the developing enteric nervous system, and predict cell-type specific expression of Hirschsprung’s disease-associated genes. Finally, using a systems approach, we identify key cell players across multiple cell lineages driving secondary lymphoid tissue formation in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. These data provide an unprecedented catalogue of intestinal cells, and new insights into cellular programs in development, homeostasis and disease.
Status-striving orientation, creative deviance engagement and employee creativity: perspective of structural strain
Authors: Liu Zhiqiang , Pan Xiaoqing , Zhu Tingting 
Published: April 2021

Purpose
This study aims to examine why and when employees engage in creative deviance to develop creativity in China. Drawing on strain theory, the authors examined creative deviance engagement as a mediator and transformational leadership as a moderator of the distinct relationships between emotional and rational status-striving orientations and radical and incremental creativity.

Design/methodology/approach
Multisource survey data were collected from 126 team leaders and 446 employees in Chinese firms. Multilevel path analysis was used to test the hypotheses.

Findings
The results show that emotional status-striving orientation relates to creative deviance engagement, which, in turn, has a stronger relationship with radical creativity than with incremental creativity. Furthermore, creative deviance engagement mediates the indirect relationships between emotional status-striving orientation and radical and incremental creativity. Moreover, transformational leadership moderates the above indirect relationships.

Originality/value
This study is among the first attempts to empirically test the distinct relationships between creative deviance engagement and radical and incremental creativity and further examine how creative deviance engagement mediates the indirect relationships between status-striving orientations and radical and incremental creativity. In addition, the boundary condition of the indirect relationships is investigated. The findings provide valuable insights for the extant literature on status and employee creativity.
Tri-optimization of building shape and envelope properties using Taguchi and constraint limit method
Authors: Lin Yaolin , Yang Wei 
Published: April 2021

Purpose
The purpose of this paper is to present a tri-optimization approach to optimize design solutions regarding the building shape and envelope properties considering their implications on thermal comfort, visual comfort and building energy consumption (EN). The optimization approach has been applied to obtain the optimal design solutions in five typical cities across all climatic regions of China.

Design/methodology/approach
The method comprises a tri-optimization process with nine main steps to optimize the three objectives (thermal comfort, visual comfort and building EN). The design variables considered are four types of building shape (pyramid, rectangular, cylindrical and dome shape) and different envelope properties (insulation thickness [INS] of external walls/roof, window type [WT] and window-to-envelop surface area ratio [WESR]). The optimization is performed by using the Taguchi and constraint limit method.

Findings
The results show that the optimal design solutions for all climatic regions favor cylindrical shape and triple-layer low-E glazing window. The highest insulation level of 150 mm is preferred in three climatic regions, and the INS of 90 mm is preferred in the other two climate regions. In total, 10% WESR is preferred in all climatic regions, except the mild region. When the constraint limit of lighting intensity requirement by Leadership in Energy and Environmental Design (LEED) is applied, the rectangular shape building is the optimal solution for those with 10% WESR.

Research limitations/implications
The method proposed in the paper is innovative in that it optimizes three different objectives simultaneously in building design with better accuracy and calculation speed.

Practical implications
Building designers can easily follow the proposed design guide in their practice which effectively bridges the gap between theory and practice. The optimal design solutions can provide a more comfortable living environment and yet less EN, which can help achieve the sustainability requirement of green buildings.

Social implications
The solutions presented in the paper can serve as a useful guide for practical building designers which creates economic and commercial impact. In addition, the theory and practical examples of the study can be used by building regulators to improve the energy-efficient building design standard in China.

Originality/value
The research is the first attempt that adopts tri-optimization approach to generate the optimal solutions for building shape and envelope design. The tri-optimization approach can be used by building designers to generate satisfactory design solutions from the architectural viewpoint and meanwhile to find combinations of the building shape and envelope properties that lead to design solutions with optimal building performance.
Collaboration in BIM-based construction networks: a qualitative model of influential factors
Authors: Oraee Mehran , Hosseini M. Reza , Edwards David , Papadonikolaki Eleni 
Published: April 2021

Purpose
The purpose of this paper is to present a modified model for collaboration in BIM-based construction networks (BbCNs). Though BIM is increasingly adopted and implemented across the construction industry, the problems associated with the lack of collaboration among teams in BbCNs remain a major hindrance to reaping the full potential of BIM. Previous studies have been conceptual in nature. This paper, therefore, attempts to modify and validate existing conceptual models that describe collaboration in BbCNs.

Design/methodology/approach
To modify the conceptual model for collaboration in BbCNs, qualitative data through semi-structured interviews with BIM experts in the industry were collected and analysed using qualitative methods including the use of NVivo software.

Findings
The proposed model includes influential factors and their sub-factors to collaboration in BbCNs, as well as considering their indicators. Findings reveal that several overlooked concepts, particularly unfavourable BIM contractual arrangements, act as the root causes of the unwillingness of team members in BbCNs to engage in collaborative efforts.

Research limitations/implications
The study's findings must be viewed in light of several limitations. First, the interviewees in this study were based in Australia hence their perceptions of BIM collaboration are reflective of the sociotechnical setting of BIM-enabled projects in this country. Also, the findings are based on the perception of experts in the field, rather than analysis of performance measures or quantitative assessment of associations among collaboration outcomes and various factors. This, however, provides the field with fertile grounds for future research.

Practical implications
The study benefits researchers by shifting the collaboration discourse in BIM-enabled projects from technology-related issues to the people and contractual-related domains. Moreover, the developed qualitative model provides industry professionals with a point of reference to improve collaboration on BIM-enabled projects.

Social implications
The study benefits researchers by shifting the collaboration discourse in BIM-enabled projects from technology-related issues to the people and contractual-related domains. Moreover, the developed qualitative model provides industry professionals with a point of reference to improve collaboration on BIM-enabled projects.

Originality/value
Arguments provided in this study highlight the necessity of considering the contractual arrangement of BIM-related projects and foster the willingness of team members to collaborate. This can be addressed using clear and comprehensive BIM execution plans and clearly explaining the role of BIM managers in the process.
Can TMT vertical pay disparity promote firm innovation performance? The moderating role of CEO power and board characteristics
Authors: Zhong Xi , Wan He , Ren Ge 
Published: April 2021

PurposeBased on the tournament theory and the principal agent theory, this study aims to empirically investigate how top management team (TMT) vertical pay disparity (the pay disparity between the CEO and non-CEO executives) influences firm innovation performance.Design/methodology/approachThis study empirically tested the hypotheses based on a sample of listed high-tech companies in China during the period between 2007 and 2018.FindingsTMT vertical pay disparity promotes innovation performance; CEO power undermines the positive effect of TMT vertical pay disparity on innovation performance; the negative moderating effect of CEO power is mitigated by board age and gender and educational levels, whereas the proportion of female directors has no such effect at any significant level.Originality/valueThis study uniquely contributes to the theoretical and empirical development of tournament theory and the principal agent theory.
Exploring antecedents of entrepreneurial intentions among females in an emerging economy
Authors: Ali Jabir , Shabir Sana , Shaikh Ateeque 
Published: April 2021

PurposeThis paper aims at identifying the antecedents of entrepreneurial intentions among females in India using the theory of planned behaviour.Design/methodology/approachThis study is based on the Adult Population Survey (APS) of the Global Entrepreneurship Monitor (GEM), which has covered 1,683 female respondents from India. The data has been analysed using simple techniques such as chi-square statistics and logistics regression. The antecedents of entrepreneurial intentions have been identified using the theory of planned behaviour.FindingsAbout 20% of the adult females have reported intention for starting an entrepreneurial venture in the country. A significant relationship emerged between the demographics of females with and without intention towards entrepreneurship. The results showed that there is a positive and significant effect of attitudes towards the behaviour, subjective norms and perceived behavioural control to the entrepreneurial intentions among females.Practical implicationsThis paper provides insights on factors affecting entrepreneurial intention among females and helps in developing a policy framework for promoting new ventures among female entrepreneurs. This also explores the possibility of future research on entrepreneurial intention in the Indian context.Originality/valueConsidering the current focus of the government in India for promoting new ventures, this piece of research can be valuable for different stakeholders in adopting a gender-based approach in implementing inclusive entrepreneurial initiatives.