bluuebunny/crossref_metadata_2025_split huggingface dataset:
PAFAH1B3 Predicts Poor Prognosis and Promotes Progression in Lung Adenocarcinoma
Authors: Tang Suping , Ni Jun , Chen Bohua , Sun Fei , Ni Songshi , Tang Zhiyuan 
Published: April 2021

Abstract
Background

Recently, increasing evidence has indicated that platelet-activating factor acetylhydrolase 1b catalytic subunit 3 (PAFAH1B3) plays an important role in several cancers. However, the role in lung adenocarcinoma (LUAD) has not been reported until now.
Methods

Expression of PAFAH1B3 in LUAD was determined by Gene Expression Profiling Interactive Analysis (GEPIA), real-time PCR (RT-PCR), Western blot and Immunohistochemical (IHC) analysis. LUAD datasets with clinical information were obtained from The Cancer Genome Atlas Program (TCGA). Chi-square test was used to investigate the correlation between PAFAH1B3 expression and clinical parameters. Cox regression and Kaplan-Meier analysis were performed to analyzed the prognostic value of PAFAH1B3. CCK-8 assay, clone formation assay, transwell invasion assay and flow cytometry were conducted to detect cell proliferation, clone formation, invasion and cell cycle. Western blot was performed to detect epithelial-to-mesenchymal transition (EMT)-related markers. Immune Cell Abundance Identifier (ImmuneCellAI) was used to analyze the effect of PAFAH1B3 on immune cell infiltration.
Results

Our study showed that PAFAH1B3 was upregulated in LUAD, and silencing PAFAH1B3 suppressed cell proliferation, colony formation, invasion and increased the cell population in G0-G1 phases in vitro. In addition, tissue microarray IHC analysis showed that the PAFAH1B3 protein level was remarkably correlated with distant metastasis, TNM stage and clinical outcome. Furthermore, multivariate cox regression analysis based on TCGA-LUAD datasets and tissue microarray indicated that PAFAH1B3 was an independent prognostic risk factor for LUAD patients. Moreover, knockdown of PAFAH1B3 inhibited EMT in LUAD cells and PAFAH1B3 mRNA expression was correlated with immune infiltrates.
Conclusion

Our studies indicate that PAFAH1B3, a prognostic risk factor, promotes proliferation, invasion and EMT and affects immune infiltrates in LUAD.
Molecular Structure, Hirshfeld Surface Analysis, Optical and Electrochemical Studies of Stable Solid Diazonium Salt of p-Sulphophenyl-3-methyl-5-pyrazolone
Authors: Shabir Ghulam , Hussain Ghulam , Saeed Aamer , Hussain Hafiz Tasawwar , Hokelek Tuncer , Erben Mauricio , Flörke Ulrich 
Published: April 2021

Abstract
The title compound is a new pyrazolone derivative which was synthesized starting from p-sulphophenyl-3-methyl-5-pyrazolone (1) by nitrosation at low temperature to afford the corresponding p-sulphophenyl-3-methyl-4-nitroso-5-pyrazolone which can exist both in nitroso (2a) and oxime tautomeric forms (2b). Reduction of the latter using zinc with hydrochloric acid furnished the 4-amino-p-sulphophenyl-3-methyl-5-pyrazolone (3). The diazotization of (3) under careful control of temperature and pH afforded the p-Sulphophenyl − 3-methyl-5-pyrazolone diazonium salt (4) which was re-crystallized from acidified ethanol to afford crystal suitable for X-ray studies. UV-visible spectrum and cyclic voltammetric studies were also carried out indicating λmax at 420 nm and HOMO-LUMMO energy gap was also calculated (Eg) of 2.95 eV. The molecular and crystal structures of the compound were clarified by single crystal X-ray diffraction indicated that it crystallizes as the sodium salt in the triclinic space group P -1, with the 4-azo-pyrazolone and the sulphophenyl groups being nearly coplanar. To get an insight to the intermolecular interactions in the crystal a Hirshfeld surface analysis was also carried out.
Do biochar and polyacrylamide have synergistic effect on net denitrification and ammonia volatilization in saline soils?
Authors: Pan Yongchun , She Dongli , Shi Zhenqi , Chen Xinyi , Xia Yongqiu 
Published: April 2021

Abstract
Salt-affected soils have poor structure and physicochemical properties, which affect soil nitrogen cycling process closely related to the environment, such as denitrification and ammonia volatilization. Biochar and polyacrylamide (PAM) have been widely used as soil amendments to improve soil physicochemical properties. However, how they affect denitrification and ammonia volatilization in saline soils is unclear. In this study, the denitrification and ammonia volatilization rates were measured in a saline soil field ameliorated with three biochar application rates (0%, 2% and 5%, w/w) and three PAM application rates (0‰, 0.4‰ and 1‰, w/w) over three years. The results showed that denitrification rates decreased by 23.63%-39.60% with biochar application, whereas ammonia volatilization rates increased by 9.82%-25.58%. The denitrification and ammonia volatilization rates decreased by 9.87%-29.08% and 11.39%-19.42% respectively, following PAM addition. However, there was no significant synergistic effect of biochar and PAM amendments on the denitrification and ammonia volatilization rates. The addition of biochar mainly reduced the denitrification rate by regulating the dissolved oxygen and electrical conductivity of overlying water and absorbing soil nitrate nitrogen. Meanwhile, biochar application increased pH and stimulated the transfer of NH4+-N from soil to overlying water, thus increasing NH3 volatilization rates. Hence, there was a tradeoff between denitrification and NH3 volatilization in the saline soils induced by biochar application. PAM reduced the denitrification rate by increasing the infiltration inorganic nitrogen and slowing the conversion of ammonium to nitrate. Moreover, PAM reduced the concentration of NH4+-N in the overlying water through absorbing soil ammonium and inhibiting urea hydrolysis, thereby decreasing NH3 volatilization rate.
The Split Friction Model - Introducing Mixed Lubrication to Curling
Authors: Ziegler Martin 
Published: April 2021

Abstract
In general, the curling stone is subject to mixed lubrication, resulting in the characteristic Stribeck -curve. As velocity increases, the friction force falls quadratically just to rise linearly yet almost flat after the minimum. In the case of a rotating curling stone this results in a torque. Due to isotropy , the lateral force arises as a delta of asymmetric friction forces opposite to the centripetal forces.
\par This in turn allows a split friction model that splits up the quadratic curve into two rather constant values for the friction force on the advancing and the retreating side below a critical velocity difference of these sides: the flee force on the advancing side must not exceed the normal force of the retreating side. Only then a curl can happen. This explains why a stone curls towards the end of the throw. \par Following basic static considerations, the stone may theoretically rest on up to three points during a throw. Each single static case is investigated. These results are discussed with additional heuristic calculations that involve Scratch-Theory. Lastly, the influence of gyroscopic precession yields a graph that reflects established experimental observations: A desired flat curve within deviations ranging from 0.80 to 1.02 meter for up to 20 rotations just to rise linearly up to 2 meters for 80 rotations.
Income Distribution of Forest Rights Transfer Cooperation Based on Game Theory: A Case of China
Authors: Zhang Wei , Qin Xian 
Published: April 2021

Abstract
Forest resources primarily characterize the terrestrial ecosystem and forest ecosystem is an important part of the global carbon cycle. Multiple participants to complete the large-scale production of forestland can effectively achieve carbon neutrality and realize the economic benefits of the forest industry, requiring far-reaching changes in the way forestland is managed. It is imminent to promote forest rights transfer between forest farmers and social capital. Based on the evolutionary game theory, we find that income distribution is a significant factor affecting the stable cooperation between business enterprises and forest farmers. To distribute income effectively within this cooperative framework, whereby farmers transfer their forest rights in different ways, we use a trapezoidal fuzzy number weighting method. This method improves the traditional Shapley value based on the investment and risk level of each participant in the cooperative alliance. Using a case study of forest rights transfer in Xin’tai city of China, we show that the improved Shapley value method can optimize the income distribution of forest rights transfer and ensure that all parties obtain long-term and stable benefits.
Subdivision of Superior Longitudinal Fasciculus in Human Neonatal Brain
Authors: Liang Wenjia , Yu Qiaowen , Wang Wenjun , Dhollander Thijs , Suluba Emmanuel , Li Zhuoran , Xu Feifei , Hu Yang , Tang Yuchun , Liu Shuwei 
Published: April 2021

Abstract
The superior longitudinal fasciculus (SLF) is a complex associative tract comprising of three distinct subdivisions in the fronto-parietal cortex, each presenting different anatomical connectivity and different functional roles. However, the subdivision of SLF was hampered by limitations of data quality and tractography methods, and many studies on white matter development often consider the SLF as a single entity. The exact anatomical trajectory and developmental status of each sub-bundle of the human SLF in neonatal period remain poorly understood. In this work, we investigated the morphological characteristics and maturation degree of SLF each branch using the diffusion MRI (dMRI) data of 40 healthy term neonates from the Developing Human Connectome Project (dHCP) database. An advanced single shell 3-tissue constrained spherical deconvolution (SS3T-CSD) algorithm was used to ensure the successful separation of the SLF three branches (SLF I, SLF II and SLF III). In addition, diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) parameters values of the three subcomponents of SLF were measured quantitatively. The fiber morphology and connectivity of SLF sub-bundles in neonatal brain were nicely revealed in our study. Furthermore, the comparison results of parameters values supported that the maturation of SLF three segments was heterochronic. Further multiple comparison results indicated that the dorsal SLF II was less mature than the ventral SLF III and the former may involve in higher-level cognitive functions. Our findings can provide new anatomical basis for early diagnosis and treatment of related diseases caused by aberrant development of SLF.
High Expression of ECM1 At Diagnosis Predicated Poor Prognosis in Adult B-Cell Acute Lymphoblastic Leukemia
Authors: Wang Weimin , Wu Feifei , Wang Fei , Wang Congcong , Lyu Chunhui , Han Lijie , Gan Silin , Ma Ping , Jiang Zhongxing , Guo Feng 
Published: April 2021

Abstract
Identifying new prognostic biomarkers and selecting treatment regimens based on them can improve outcomes of adult patients with B-cell acute lymphoblastic leukemia (B-ALL). Extracellular matrix protein 1(ECM1) is a glycoprotein involved in many biologic processes. Here we aimed to determine the expression and the prognostic value of ECM1 in B-ALL. One hundred and six newly-diagnosed B-ALL patients (seventy-one adult patients and thirty-five children patients) were enrolled from March 2013 to December 2018. The ECM1 transcript levels were significantly different among different disease state. Among 71 adult B-ALL patients, ECM1-high group has a higher initial platelet count( p=0.004)，more BCR-ABL fusion positive patients (p=0.004) and poor risk patients (p=0.021) than ECM1-low group, whereas no significant relationship with any other clinical characteristics was observed. ECM1-high group had significantly lower 3-year OS (11.4% vs. 36.1%, p=0.015) and 3-year RFS (9.7% vs. 29.4%, p=0.047) compared to ECM1-low group. In the multivariate Cox regression model, high ECM1 expression was an independent adverse prognostic factor for OS(HR=3.0(1.4-6.6), p=0.007). Our data demonstrated that the expression of ECM1 could serve as a novel prognostic biomarker for adult patients with B-ALL.
Title: Barriers and facilitators experienced by osteopaths in implementing a biopsychosocial (BPS) framework of care when managing people with musculoskeletal pain – A mixed methods systematic review
Authors: Sampath¹ Kesava Kovanur , Darlow² Ben , Tumilty³ Steve , Shillito Warwick , Hanses Melissa , Devan⁴ Hemakumar , Thomson Oliver P. 
Published: April 2021

Abstract
Background: Clinical practice guidelines commonly recommend adopting a biopsychosocial (BPS) framework by practitioners managing musculoskeletal (MSK) pain. However, it remains unclear how osteopaths implement a BPS framework in the management of MSK pain. Hence, the objective of this review was to systematically appraise the literature on the current practices, barriers and facilitators experienced by osteopaths in implementing a BPS framework of care when managing people with MSK pain.Methods: The following electronic databases from January 2005 to August 2020 were searched: PubMed, CINAHL, Science Direct, Google Scholar, ProQuest Central and SCOPUS. Two independent reviewers reviewed the articles retrieved from the databases to assess for eligibility. Any studies (quantitative, qualitative and mixed methods) that investigated the use or application of the BPS approach in osteopathic practice were included in the review. The critical appraisal skills program (CASP) checklist was used to appraise the qualitative studies and the Mixed Methods Appraisal Tool (MMAT) was used to appraise quantitative or mixed methods studies. Advanced convergent meta-integration was used to synthesise data from quantitative, qualitative and mixed methods studies.Results: a total of 6 articles (two quantitative, three qualitative and one mixed methods) were included in the final review. While two key concepts (current practice and embracing a BPS approach) were generated using advanced meta-integration synthesis, two concepts (barriers and enablers) were informed from qualitative only data.Discussion: Our review finding showed that current osteopathic practice occurs within in the biomedical model of care. Although, osteopaths are aware of the theoretical underpinnings of the BPS model and identified the need to embrace the BPS model, various barriers exist that stop osteopaths BPS model use in osteopathic clinical practice. Ongoing education and/or workshops may be necessary to enable osteopaths to implement a BPS approach.
Effective electrocatalytic elimination of chloramphenicol: Mechanism, degradation pathway, and toxicity assessment
Authors: Liu Haiyang , Lv Yihan , Zhang Ya-nan , Zhang Yushu , Qu Jiao , Dong Deming , Wang Zhaojun , Hua Xiuyi 
Published: April 2021

Abstract
The residual antibiotics in different environmental media pose a serious threat to human health and the ecosystem. The high-efficient elimination of antibiotics is one of the foremost works. In this study, chloramphenicol (CAP) was eliminated efficiently by electrocatalytic advanced oxidation process with carbon nanotubes/agarose/indium tin oxide (CNTs/AG/ITO) electrode. The influences of different experimental parameters on the degradation efficiency were systematically studied. Under the optimal conditions (4 V potential, 10 wt% CNTs dosage, and pH = 10), the maximum degradation efficiency of CAP (20 mg L− 1) achieved 88% within 180 min. Besides, the electrocatalytic degradation pathway and mechanism for CAP were also investigated, •O2− played a major role in the process of electrocatalytic degradation. Based on the QSAR (quantitative structure-activity relationship) model, the toxicities of CAP and identified intermediates were analyzed. Compared with the parent compound, the maximal chronic toxicity of intermediate ((E)-3-(4-nitrophenyl)prop-1-ene-1,3-diol) for daphnid increased 197-fold. Besides, the hybrid toxicity of the degradation system was further confirmed via disk agar biocidal tests with Escherichia coli ATCC25922, which changed slightly during the degradation process. Based on the above results, it is worth noting that the degradation pathway and toxicity assessment should be paid more attention to the treatment of antibiotic wastewater.
Monterey Excursion Events in the Middle Miocene Carbonate Rock Succession, Eastern Iraq
Authors: Ali Mustafa A. , Al-Mashaikie Sa'ad Z. , Othman Arsalan Ahmed 
Published: April 2021

Abstract
The global Monterey excursions are recognized in many deep-sea, and recently in shallow water depth records during the Miocene epoch. We analyzed the δ 13C and δ 18O to investigate the relationship between the lithostratigraphic units and the deviation of the stable isotopes. The results show that there is a significant relationship between the depositional energy changes and both of δ 13C and δ 18O. The present paper is deals with variability of the primary isotopic composition of seawater in the shallow water zone. Sedimentary successions of middle Miocene outcropping in the eastern part of Iraq provide an excellent archive of the oxygen isotope events (Mi‐events), additional details about Mi 2 and Mi 3 zones of glaciations and refine the Monterey Excursion, carbonate isotope maxima (CM‐events). Facies analysis revealed several microfacies could be distinguished, reflecting depositional environments including; lagoon, back reef-reef, shallow open marine, and shoal environments. The Jeribe Formation is a transgression unit belonging to the stage Ap11 tectonostratigraphy Megasequence. The Formation was deposited in the outer platform margin of unstable shelf of the Arabian Plate and significant of this formation, which is deposited during the final phase of Tethys sea closure. The stable isotope (18O/16O and 13C/12C) provide an excellent archive of the paleoceanography for the Neogene (middle Miocene Transgression) of the Neo-Teythys. Oxygen and carbon isotopic stratigraphy for bulk-rock were analyzed in order to distinguished Monterey Event and climate optima.
Comparison of the Inhibitory Effect of PEG-Liposomal and Conventional Doxorubicin on Migration and Extravasation Efficacy on Canine Osteosarcoma Cell Line- in Vitro and Ex Ovo Studies.
Authors: Walewska Magdalena Jolanta , Małek Anna , Taciak Bartłomiej , Wojtalewicz Anna , Wilk Sylwia , Wojtkowska Agata , Koczywąs Katarzyna Zabielska- , Lechowski Roman 
Published: April 2021

Abstract
The chick chorioallantoic membrane (CAM) assay has long been used to study the effects of drugs on angiogenesis or evaluate cancer cell invasiveness by quantifying in vivo rates of cancer cell extravasation. Extravasation plays a crucial role in the metastatic cascade, whereby circulating cancer cells derived from the primary tumor cross the endothelial barrier to reach the target metastatic site. Accordingly, we adapted an ex ovo model to study the anti-extravasation efficiency of anticancer drugs.
The drugs investigated include conventional and PEG-liposomal doxorubicin. The conventional form is commonly used in chemotherapy protocols for canine appendicular osteosarcoma (OSA), although it has no specific biodistribution and a low therapeutic index. For this reason, this study compared the effects of conventional and PEG-liposomal doxorubicin on cytotoxicity and migration inhibition in the in vitro environment. Cytotoxicity was evaluated by the MTT assay, Annexin V staining and the Draq 7 test; the inhibition of migration was analyzed using the scratch assay test. Moreover the inhibitory effect of study drugs on cancer cell extravasation was analyzed in the in vivo conditions, on the ex ovo model.
The results of experiments performed showed that PEG-liposomal doxorubicin has a higher inhibitory effect on the in vitro migration of canine OSA (p ≤ 0.05). Ex ovo research revealed both drugs elicited a high efficiency for inhibiting the extravasation of canine OSA (p< 0.0001). Therefore PEG-liposomal doxorubicin may be considered as a potentially useful anti-metastatic agent in canine osteosarcoma due to its inhibitory effect on both the migration and extravasation of the D-17 cell line.
Cyanine-based Fluorescent Probe for Cyanide Ion Detection
Authors: Gosi Mahesh , Marepu Nagaraju , Sunandamma Yeturu 
Published: April 2021

Abstract
Cyanine-based probe-possessing indolium and indole unit was synthesized in two-step with easy available raw material: a potential probe for the cyanide ion detection. The detecting ability of the probe was investigated and confirmed by a visual and instrumental approach. A noticeable color change from orange to colorless obtained only for cyanide ion and other added ions does not impart any changes visually and through UV and Fluorescence technique. To confirm the mechanism of sensing 1H-NMR recorded. From the result, the peak belonging to N -methyl displayed an upfield shift from 4.01 δ ppm to 2.74 δ ppm due to the disappearance of indolium ion and the olefin protons peaks were shifted from 7.19 to 6.17 and 8.70 to 7.20 δ ppm confirms the nucleophilic addition of cyanide ion to the probe. Test kit from filter paper prepared for the real-time monitoring cyanide ion. The prepared strip is effective in detecting cyanide ion with a visual color change.
An Intelligent Modeling and Controller design for Clarifier process: A Metaheuristic Algorithm approach.
Authors: Rajalakshmi M , Karthik C , Arunprasad V , Saravanakumar G , Pandiyan Sanjeevi 
Published: April 2021

Abstract
This paper concentrates on the modeling and control of the sugar industry's nonlinear clarifier process. Since the sugar industry's clarification mechanism is complex and nonlinear, it is therefore important to obtain the exact model with identification methods. Using the normal modeling technique, the basic model of the complex process is obtained and further improved to make the model act like the actual system. The most accurate model from the algorithms is analysed using metaheuristic algorithms such as Genetic Algorithm (GA), Particle Swarm Optimization (PSO), and State Transition Algorithm (STA). The proposed STA design to the clarifier model provides the maximum fitness. The clarifier model derived from State Transition Algorithm (STA) behaves more similar to the actual clarifier process by capturing the principle dynamic qualities of the process. Simulations have demonstrated that STA is an optimum algorithm for the clarifier process than the other algorithms. From the results, it is inferred that the controllers introduced in this study, can be utilized to accomplish a better performance than the standard controller design, and during the control of any nonlinear procedure and STA is extremely helpful in modeling a nonlinear process.