bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Key Histone Modifications and Hub Genes for Colorectal Cancer Metastasis
Authors: Zhai Yuan-Yuan , Li Qian-Zhong , Chen Ying-Li , Zhang Lu-Qiang 
Published: August 2021

Background:
Epithelial-Mesenchymal Transition (EMT) and its reverse Mesenchymal-
Epithelial Transition (MET) are essential for tumor cells metastasis. However, the effect of epigenetic
modifications on this transition is unclear.

Objective:
We aimed to explore the key histone modifications and hub genes of EMT/MET during Colorectal
Cancer (CRC) metastasis.

Method:
The differentially expressed genes and differentially histone modified genes were identified.
Based on the histone modification features, the up- and down-regulated genes were predicted by Random
Forest algorithm. Through protein-protein interaction network and Cytoscape analysis, the hub
genes with histone modification changes were selected. GO, KEGG and survival analyses were performed
to confirm the importance of the hub genes.

Results:
It was found that H3K79me3 plays an important role in EMT/MET. And the 200-300bp and
400-500bp downstream of TSS may be the key regulatory regions of H3K79me3. Moreover, we found
that the expression of the hub genes was down-regulated in EMT and then up-regulated in MET. And
the changes of the hub genes expression were consistent with the changes of H3K79me3 signal in the
specific regions of the genome. Finally, the hub genes KRT8 and KRT18 were involved in the metastasis
process and were significantly related to the survival time.

Conclusion:
H3K79me3 may be crucial for EMT/MET, and the hub genes KRT8 and KRT18 may be
the key genes in this process.
Dehydrocostus Lactone Induces Apoptosis and Cell Cycle Arrest through Regulation
of JAK2/STAT3/PLK1 Signaling Pathway in Human Esophageal Squamous Cell
Carcinoma Cells
Authors: Yang Ganghua , Sheng Binwu , Li Ruixiang , Xu Qinhong , Zhang Lei , Lu Zhengyang 
Published: August 2021

Background:
Dehydrocostus lactone (DEH), one of the sesquiterpene lactones, has shown extensive pharmaceutical
activities, including anti-cancer activity. However, its effects on human esophageal squamous cell carcinoma
(ESCC) cells are still unknown.

Objective:
To investigate the effect of DEH on ESCC cells and the underling molecular mechanisms.

Method:
The cell proliferation was tested using CCK-8 and colony formation assay. Apoptosis was analyzed by flow
cytometry, hoechst staining and caspase-3 activity assay. Cell cycle was analyzed by flow cytometry. IL-6 (STAT3
activator) was used to activate JAK2/STAT3 pathway. Immunofluorescence assay was performed to detect intracellular
location of STAT3. SiRNA transfection was performed to knock down the expression of PLK1. The protein expression
was analyzed by western blotting assay.

Result:
DHE treatment significantly reduced the viability of ESCC cells through apoptosis induction and cell cycle
arrest. Furthermore, DHE treatment significantly inhibited the phosphorylation of JAK2 and STAT3. IF assay showed
that the distribution of STAT3 in the nucleus was decreased by DHE treatment. In addition, coculture with IL-6 significantly
prevented the inhibition of phosphorylation of JAK2 and STAT3 by DHE treatment and partly reversed the
effect of DHE on ESCC cells. Moreover, DHE treatment significantly down-regulated the expression of PLK1, which
was partly reversed by IL-6 coculture. Finally, knock down of PLK1 using siRNA reduced the viability of ESCC cells
and induced apoptosis and cell cycle arrest

Conclusion:
Our study demonstrated that DHE has a potent anti-cancer effect on ESCC cells through apoptosis induction
and cell cycle arrest via JAK2/STAT3/PLK signaling pathway.
Advancements in the Use of Platinum Complexes as Anticancer Agents
Authors: Chawla Pooja A. , Sharma Rajiv , Singh Vikram Jeet 
Published: August 2021

Background:
The platinum (II) complexes as anticancer agents have been well explored for the development
of novel analogs. Yet, none of them achieved clinical importance in oncology. At present, anticancer compounds
containing platinum (II) complexes have been employed in the treatment of colorectal, lung, and genitourinary tumors.
Among the platinum-based anticancer drugs, Cisplatin (cis-diamine dichloroplatinum (II), cis-[Pt(NH3)2Cl2]) is one of
the most potent components of cancer chemotherapy. The nephrotoxicity, neurotoxicity and ototoxicity, and platinum
compounds associated resistant cancer are some major disadvantages.

Objective:
With the rapidly growing interest in platinum (II) complexes in tumor chemotherapy, researchers have
synthesized many new platinum analogs as anticancer agents that show better cytotoxicity, and less off-target effects
with less cellular resistance. This follows the introduction of oxaliplatin, water-soluble carboplatin, multinuclear platinum
and newly synthesized complexes, etc.

Method:
This review emphasizes recent advancements in drug design and development, the mechanism of platinum
(II) complexes, their stereochemistry, current updates, and biomedical applications of platinum-based anticancer
agents.

Conclusion:
In the last few decades, the popularity of platinum complexes as potent anti-cancer agents has risen as
scientists have synthesized many new platinum complexes that exhibit better cytotoxicity coupled with less off-target
effects.
Electrochemotherapy in the Treatment of Kaposi’s Sarcoma: A Single
Centre Cohort Study and a Comparison with the Literature Data
Authors: Savoia Paola , Cammarata Edoardo , Giorgione Roberto , Andreassi Marco , Airoldi Chiara 
Published: August 2021

Background:
Kaposi's sarcoma (KS) is a tumor of endothelial derivation, which primarily affects the skin and is mainly related to the type 8 Human Herpesvirus (HHV8). Its onset is favored by immunosuppression, although the most common form is the classic or spo-radic KS mainly developing in elderly men of Mediterranean and Eastern European origin. Dif-ferent therapeutic options are available, depending on the clinical variant, progression pattern, and comorbidities. The treatment of localized forms includes surgical excision, laser treatment, cryosurgery, radiotherapy, imiquimod 5%, and intra-lesion injection of cytotoxic drugs; on the other hand, the treatment of widespread disease encompasses radiotherapy and chemotherapy.

Methods:
In this scenario, Electrochemotherapy (ECT), has shown to be an effective alternative to traditional treatment for disseminated KS skin lesions. The rationale of ECT relies on the lo-cal application of short, high-voltage electric pulses, able to open transient pores in the cell membrane (reversible electroporation that increases the delivery of some poorly permeant cyto-toxic agents into the cytosol.

Results:
Herein, we performed a retrospective analysis on 9 KS patients treated with ECT at our center between June 2016 and January 2020. The rate of Complete Response (CR) was 77.8% after the first cycle of treatment and 88.9% after the second course, with an overall response (OR) of 100%.

Conclusion:
Sustained local control of treated lesions was present in 77.8% of patients 6 months after the treatment and all of them reported only mild local toxicity, together with an excellent functional and cosmetic outcome, in agreement with data obtained from the compari-son with the recent literature.
Metabolite Biomarkers of CKD Progression in Children
Authors: Denburg Michelle R. , Xu Yunwen , Abraham Alison G. , Coresh Josef , Chen Jingsha , Grams Morgan E. , Feldman Harold I. , Kimmel Paul L. , Rebholz Casey M. , Rhee Eugene P. , Vasan Ramachandran S. , Warady Bradley A. , Furth Susan L. ,   for the CKD Biomarkers Consortium
Published: August 2021

Background and objectives
Metabolomics facilitates the discovery of biomarkers and potential therapeutic targets for CKD progression.

Design, setting, participants, & measurements
We evaluated an untargeted metabolomics quantification of stored plasma samples from 645 Chronic Kidney Disease in Children (CKiD) participants. Metabolites were standardized and logarithmically transformed. Cox proportional hazards regression examined the association between 825 nondrug metabolites and progression to the composite outcome of KRT or 50% reduction of eGFR, adjusting for age, sex, race, body mass index, hypertension, glomerular versus nonglomerular diagnosis, proteinuria, and baseline eGFR. Stratified analyses were performed within subgroups of glomerular/nonglomerular diagnosis and baseline eGFR.

Results
Baseline characteristics were 391 (61%) male; median age 12 years; median eGFR 54 ml/min per 1.73 m2; 448 (69%) nonglomerular diagnosis. Over a median follow-up of 4.8 years, 209 (32%) participants developed the composite outcome. Unique association signals were identified in subgroups of baseline eGFR. Among participants with baseline eGFR ≥60 ml/min per 1.73 m2, two-fold higher levels of seven metabolites were significantly associated with higher hazards of KRT/halving of eGFR events: three involved in purine and pyrimidine metabolism (N6-carbamoylthreonyladenosine, hazard ratio, 16; 95% confidence interval, 4 to 60; 5,6-dihydrouridine, hazard ratio, 17; 95% confidence interval, 5 to 55; pseudouridine, hazard ratio, 39; 95% confidence interval, 8 to 200); two amino acids, C-glycosyltryptophan, hazard ratio, 24; 95% confidence interval 6 to 95 and lanthionine, hazard ratio, 3; 95% confidence interval, 2 to 5; the tricarboxylic acid cycle intermediate 2-methylcitrate/homocitrate, hazard ratio, 4; 95% confidence interval, 2 to 7; and gulonate, hazard ratio, 10; 95% confidence interval, 3 to 29. Among those with baseline eGFR <60 ml/min per 1.73 m2, a higher level of tetrahydrocortisol sulfate was associated with lower risk of progression (hazard ratio, 0.8; 95% confidence interval, 0.7 to 0.9).

Conclusions
Untargeted plasma metabolomic profiling facilitated discovery of novel metabolite associations with CKD progression in children that were independent of established clinical predictors and highlight the role of select biologic pathways.
Regolith Excavation Performance of a Screw-propelled Vehicle
Authors: Green Marko , McBryan Teresa , Mick Darwin , Nelson David , Marvi Hamid 
Published: August 2021

Excavation of regolith is the enabling process for many of the in-situ
resource utilization (ISRU) efforts that are being considered to aid in
the human exploration of the moon and Mars. Most proposed planetary
excavation systems are integrated with a wheeled vehicle, but none yet
have used a screw-propelled vehicle which can significantly enhance the
excavation performance. Therefore, CASPER, a novel screw-propelled
excavation rover is developed and analyzed to determine its
effectiveness as a planetary excavator. The excavation rate, power,
velocity, cost of transport, and a new parameter, excavation transport
rate, are analyzed for various configurations of the vehicle through
mobility and excavation tests performed in silica sand. The optimal
configuration yielded a 30 kg/hr excavation rate and 10.2 m/min traverse
rate with an overall system mass of 3.4 kg and power draw of less than
30 W. These results indicate that this architecture shows promise as a
planetary excavation because it provides significant excavation
capability with low mass and power requirements.

Corresponding author(s) Email: hmarvi@asu.edu
Dependence of the velocity changes of secular variations on the position of observatory and time
Authors: Sumaruk T. P. , Sumaruk P. V. 
Published: August 2021

According to the data of world observatories net secular variations of geomagnetic fields from internal and outer sources have been studied. Averaged 3-year data have been used for this purpose. Procedure of calculations of secular variations from internal and outer sources according to observatories data has been submitted. 1979 has been chosen as a zero level for accounting secular variations from outer sources because the sign of the large-scale magnetic field has changed this year. It has been shown that the value of secular variations from outer sources is different for different regions and increases with the growth of the latitude of magnetic observatory. Maximal values of secular variations are observed in the northern polar cap as well as at the longitudes of the eastern focus of secular variation. It has been shown that at the DIK, CSS, TIK observatories secular variations have maximal values.
Groups of observatories have been segregated with symmetric and asymmetric changes of secular variation comparing to 1979. Symmetric changes of secular variation during two Hail’s cycles are observed at the observatories in circumpolar area (ALE, NAL, BJN), in auroral and middle latitudes. Maximal asymmetry of secular variation is observed at the observatories GDH, BLC, FCC, as well as at certain subauroral observatories and the regions with raised seismic activity.
Secular variation from outer sources depends on the value of the large scale magnetic field of the Sun. The value of secular variation from the inner sources has been modulated by the outer sources and depends on special features of underlying surfaces of the observatories, induction currents in particular.
Legal status of minors held in educational colonies in the Russian Federation
Authors: Alontseva Dina Viktorovna , Lavrishcheva Olga Anatolyevna 
Published: August 2021

The article is devoted to the analysis of the theoretical and legal bases regulating the legal status of minors who are in places of deprivation of liberty. The methodological basis of the research is made up of general scientific, private and special methods of cognition. The general scientific dialectical method allowed us to consider the problem of legal relations arising in the sphere of securing the legal status of minors who have been sentenced to imprisonment; to conduct a comparative analysis of international and domestic legislation in regulating the designated topic. Using the methods of formal logic, the transformations in the sphere of regulating the legal status of juvenile convicts at the present stage are characterized, defects in regulatory regulation are identified. The methods of scientific knowledge will help to conduct a study of the current legislation for its compliance with the problem under consideration.
Патологічна деформація та тромбоз лівої внутрішньої сонної артерії. Випадок із практики
Authors: Кобза I.I. , Кобза Т.І. , Мота Ю.С. , Кирилич І.В. 
Published: August 2021

Ділянки патологічних вигинів сонних артерій небезпечні виникненням тромбоутворення з подальшим перекриттям кровоплину. У даному клінічному спостереженні у хворої віком 54 роки, з діагнозом пристінкового тромбозу сонних артерій патологічна звивистість внутрішньої сонної артерії перешкодила подальшому просуванню тромбоембола через кровоносне русло, що дозволило уникнути небезпечних ускладнень при своєчасному проведенні хірургічного втручання.
Ключові слова: патологічна звивистість, тромбоз, ішемічний інсульт, ретроградна каротидна тромбендартеректомія.
Досвід використання малоінвазивних методів лікування хворих із варикозною хворобою нижніх кінцівок, ускладненою гострим тромбофлебітом підшкірних вен
Authors: Перцов В.І. , Мамунчак О.В. , Перцов І.В. 
Published: August 2021

У роботі наведені результати амбулаторного малоінвазивного лікування пацієнтів із варикозною хворобою нижніх кінцівок, ускладненою тромбофлебітом. Встановлено, що радіочастотна абляція в поєднанні з мініфлебектомією є ефективним методом лікування за рахунок радикального видалення патологічного вогнища, одночасної ліквідації загрози рецидивуючого плину захворювання, прискорення термінів реабілітації, має гарний косметичний ефект та може бути альтернативою відкритих хірургічних втручань.Ключові слова: варикозна хвороба, тромбофлебіт, радіочастотна абляція, флебектомія.
Improvement of Weeds Management System and Fertilisers Application in Winter Wheat (Triticum aestivum L.) Cultivation Technologies
Authors: Rebouh Nazih Yacer , Latati Morad , Polityko Peter , Meisam Zargar , Kavhiza Nyasha John , Garmasch Nina , Pakina Elena , Lyshko Marina , Engeribo Albert , Orujov Elchin , Vvedenskiy Valentin 
Published: August 2021

Abstract
Wheat production plays a central role in the Russian agricultural system and significant land area is dedicated to this strategic crop. However, the wheat enterprise is highly constrained by weed interference which cause serious yield losses hence minimizing production income. The main objective of the study was to assess the efficacy of three various cultivation technologies as basic, intensive, and highly intensive systems on wheat biological efficiency. Three weed species Echinochloa crus-galli, Stellaria media, and Viola arvensis, and three winter wheat (Triticum aestivum L.) varieties Moscovskaya 40 (V1), Nemchinovskaya 17 (V2) and Nemchinovskaya 85 (V3) were studied. The data was analysed as a randomized complete block design with three replicates. Weed density, biological efficiency, yield performances, and selected qualitative parameters (measured through protein and gluten contents) were determined as affected by different cultivation technologies. The results showed that the high intensive cultivation technology (T3) was the most effective in reducing weed infestation levels as follows (0.3 plant/m2
Echinochloa crus-galli, 0.5 plant/m2
Stellaria media and 0.4 plant/m2
Viola arvensis) with biological efficiency of 96%, while 81% and 90% were recorded with basic and intensive cultivation system respectively. Moreover, the highest wheat yield 10.6 t/ha was obtained by T3, with the greatest grain quality 5% higher than basic cultivation technology designated in T1. The results were variety-dependent revealing the intrinsic genetic performances and the different patterns of high competitive ability. The current results open real opportunities concerning the implementation of potent wheat production systems.
Examples of non connective C
*-algebras
Authors: Gąsior Anna , Szczepański Andrzej 
Published: August 2021

Abstract
This paper investigates the problem of the existence and uniqueness of solutions under the generalized self-similar forms to the space-fractional diffusion equation. Therefore, through applying the properties of Schauder’s and Banach’s fixed point theorems; we establish several results on the global existence and blow-up of generalized self-similar solutions to this equation.
Die Czernowitzer Sprachkonferenz von 1908 und ihr Nachklang in der Presse am Beispiel des Periodikums Czernowitzer Tagblatt
Authors: Solomon Francisca 
Published: August 2021

Abstract
The first Conference for the Yiddish Language of 1908 was a highly significant event in the history of Yiddish language and culture, which became known in the literature as the Czernowitz Language Conference [Yidd. „di konferents far der yidisher shprakh“]. This conference was held in the city of Czernowitz from August 30 to September 3 and united prominent representatives of the worldwide Yiddish movement and, thus, triggered a significant impulse to the development of an energetic Yiddish-speaking constellation. The conference manifested awareness of the importance of Yiddish language and culture as a breeding ground for the survival of traditional “(Eastern) Jewish” values. Within this framework the debates regarding the cultivation of the Yiddish language have been intensified through reflective and resolute actions with the aim of releasing it from the stigma of jargon.
Lucian Blaga und das deutsche Gymnasium im siebenbürgischen Mühlbach (1902–1906)
Authors: Nistor Adina-Lucia 
Published: August 2021

Abstract
The present study plans on highlighting aspects of completing the 4-year middle school term, which was in German, by the poet, writer, philosopher and playwright Lucian Blaga; more specifically aspects on following German schools in Blaga’s family, his early school days, stories about the educator Roth and the teacher Hans Wolf, on the location of the building and the atmosphere in the school, on the contact with the German culture and language which subsequently marked his personality and his work. At the heart of this presentation lies the autobiographical writing “The chronicle and the song of the ages”, published posthumously by “Editura Tineretului din Bucureşti” (“The Youth’s Publishing House from Bucureşti”) in 1965.
Die Hebamme und ihre Benennungen im Siebenbürgisch-Sächsischen
Authors: Haldenwang Sigrid 
Published: August 2021

Abstract
This article covers midwives as such and their designations in the Transylvanian-Saxon vernaculars in detail with emphasis on the early documentary evidence in the first half of the 16th century as well as from the 18th century. The lexemes correlate their respective categories of word formation and show descriptive series of synonyms depicting at the same time the composition of the Transylvanian-Saxon vocabulary. Comments on the etymology of the word formations as well as on the midwife‘s profession are also included. The terms are taken from the Transylvanian­Saxon Dictionary and the NorthTransyl vanian Saxon Dictionary as well as the specialist literature on vernacular.
Ontische1 und pragmatische Fragen zur mittelalterlichen Textsorte Testament aus textlinguistischer Perspektive
Authors: Smereka Joanna 
Published: August 2021

Abstract
In my contribution it’s about testaments made by german citizen of Cracow. The material is based on texts of last wills of people representing a minority of citizen of Cracow, the patrician families. The tastators where drawn dealer and trademans, which also pushing the development of the region. The texttype testament often marginalized by linguistics. I aske in opposite of this from the perspective of ontology and pragmatism: What is the deeper linguistic structure of this relative formalized text-type. I show in my analysis the texts of a last will include not only declarative (resp. indirect declarative) acts of speech but also a variety of assertive and directive elements. I don’t want to decide the justice of an act of speech but I have a catalogue of repetitive facultative phrases and combine this with particular narrations of certain paragraphs of testaments. This element not only refers to the private sphere of the tastators (conflicts or emotions) but uncover the social, educational and in part religious attitude of the testators. And so at least fully developed testaments let understood as selfreports.