bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary observations on abundance and distribution of fish fauna in a canyon of the Bay of Biscay (ICES Division 8c)
Authors: Diez G. , Arregi L. , Basterretxea M. , Cuende E. , Oyarzabal I. 
Published: February 2021

AbstractThe changes in abundance and biodiversity of deep-sea fish fauna are described based on an annual deep-water longline survey with data collected during the period 2015–2019 in the Basque Country continental Slope (ICES Division 8c). The sampling scheme included hauls in four 400 m strata, from 650–2250 m deep. The DST sensors installed in the main line have allowed us to set an accurate soak time for each haul, and they were used to calculate fishing effort and CPUE by haul. The catchability of the fishing gear indicated that 15% of the total hooks deployed in the five-year period were able to fish, and that the bottom longline was very effective in fishing a wide number of different species in all depth ranges. The fishing gear caught 14 different species of sharks (13 deepwater and one pelagic), two chimaeras and nine teleosts. The abundance and biomass registered on the hooks attached to the bottom were between three and four times higher than in the floating sections, and the highest CPUE and biomass were recorded between 1051–1450 m, from 2015 to 2017, and in the 1451–1850 m strata, but they do not show any clear trend throughout the five years of the series.
Genetic contributions to anxiety disorders: where we are and where we are heading
Authors: Ask Helga , Cheesman Rosa , Jami Eshim S. , Levey Daniel F. , Purves Kirstin L. , Weber Heike 
Published: February 2021

AbstractAnxiety disorders are among the most common psychiatric disorders worldwide. They often onset early in life, with symptoms and consequences that can persist for decades. This makes anxiety disorders some of the most debilitating and costly disorders of our time. Although much is known about the synaptic and circuit mechanisms of fear and anxiety, research on the underlying genetics has lagged behind that of other psychiatric disorders. However, alongside the formation of the Psychiatric Genomic Consortium Anxiety workgroup, progress is rapidly advancing, offering opportunities for future research.Here we review current knowledge about the genetics of anxiety across the lifespan from genetically informative designs (i.e. twin studies and molecular genetics). We include studies of specific anxiety disorders (e.g. panic disorder, generalised anxiety disorder) as well as those using dimensional measures of trait anxiety. We particularly address findings from large-scale genome-wide association studies and show how such discoveries may provide opportunities for translation into improved or new therapeutics for affected individuals. Finally, we describe how discoveries in anxiety genetics open the door to numerous new research possibilities, such as the investigation of specific gene–environment interactions and the disentangling of causal associations with related traits and disorders.We discuss how the field of anxiety genetics is expected to move forward. In addition to the obvious need for larger sample sizes in genome-wide studies, we highlight the need for studies among young people, focusing on specific underlying dimensional traits or components of anxiety.
Feminist Mobilization and the Abortion Debate in Latin America: Lessons from Argentina
Authors: Daby Mariela , Moseley Mason W. 
Published: February 2021

AbstractWhen Argentine president Mauricio Macri announced in March 2018 that he supported a “responsible and mature” national debate regarding the decriminalization of abortion, it took many by surprise. In a Catholic country with a center-right government, where public opinion regarding abortion had hardly moved in decades—why would the abortion debate surface in Argentina when it did? Our answer is grounded in the social movements literature, as we argue that the organizational framework necessary for growing the decriminalization movement had already been built by an emergent feminist movement of unprecedented scope and influence: Ni Una Menos. By expanding the movement's social justice frame from gender violence to encompass abortion rights, feminist activists were able to change public opinion and expand the scope of debate, making salient an issue that had long been politically untouchable. We marshal evidence from multiple surveys carried out before, during, and after the abortion debate and in-depth interviews to shed light on the sources of abortion rights movements in unlikely contexts.
Tort reform: do details matter?
Authors: Childers Rachel G. 
Published: February 2021

AbstractOne explanation for increases in health care costs has been malpractice lawsuits. States have introduced several types of tort reforms to control increases in health care costs. This paper adds to the literature by examining how the differences in joint and several liability (JSL) reforms affect the state-specific growth rate in health care expenditures. Additionally, the paper addresses the potential for a fundamental difference between states that pass different types of liability reforms. The results show that JSL reforms that limit joint liability based on percentage of blame have statistically and economically significant impacts on health care expenditure growth rates.
The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury
Authors: Bao Yi-Ni , Dai Wen-Ling , Fan Ji-Fa , Ma Bin , Li Shan-Shan , Zhao Wan-Li , Yu Bo-Yang , Liu Ji-Hua 
Published: February 2021

AbstractDopamine D1 receptor (D1DR) and D2 receptor (D2DR) are closely associated with pain modulation, but their exact effects on neuropathic pain and the underlying mechanisms remain to be identified. Our research revealed that intrathecal administration of D1DR and D2DR antagonists inhibited D1–D2DR complex formation and ameliorated mechanical and thermal hypersensitivity in chronic constriction injury (CCI) rats. The D1–D2DR complex was formed in the rat spinal cord, and the antinociceptive effects of D1DR and D2DR antagonists could be reversed by D1DR, D2DR, and D1–D2DR agonists. Gαq, PLC, and IP3 inhibitors also alleviated CCI-induced neuropathic pain. D1DR, D2DR, and D1–D2DR complex agonists all increased the intracellular calcium concentration in primary cultured spinal neurons, and this increase could be reversed by D1DR, D2DR antagonists and Gαq, IP3, PLC inhibitors. D1DR and D2DR antagonists significantly reduced the expression of p-PKC γ, p-CaMKII, p-CREB, and p-MAPKs. Levo-corydalmine (l-CDL), a monomeric compound in Corydalis yanhusuo W.T. Wang, was found to obviously suppress the formation of the spinal D1–D2DR complex to alleviate neuropathic pain in CCI rats and to decrease the intracellular calcium concentration in spinal neurons. l-CDL-induced inhibition of p-PKC γ, p-MAPKs, p-CREB, and p-CaMKII was also reversed by D1DR, D2DR, and D1–D2DR complex agonists. In conclusion, these results indicate that D1DR and D2DR form a complex and in turn couple with the Gαq protein to increase neuronal excitability via PKC γ, CaMKII, MAPK, and CREB signaling in the spinal cords of CCI rats; thus, they may serve as potential drug targets for neuropathic pain therapy.
Antioxidant treatment ameliorates prefrontal hypomyelination and cognitive deficits in a rat model of schizophrenia
Authors: Maas D. A. , Eijsink V. D. , van Hulten J. A. , Panic R. , De Weerd P. , Homberg J. R. , Vallès A. , Nait-Oumesmar B. , Martens G. J. M. 
Published: February 2021

AbstractCognitive dysfunction in schizophrenia (SZ) is thought to arise from neurodevelopmental abnormalities that include interneuron hypomyelination in the prefrontal cortex (PFC). Here we report that RNA-sequencing of the medial (m)PFC of the APO-SUS rat model with SZ-relevant cognitive inflexibility revealed antioxidant metabolism as the most-enriched differentially expressed pathway. Antioxidant-related gene expression was altered throughout postnatal development and preceded hypomyelination. Furthermore, reduced glutathione levels and increased mitochondria numbers were observed in the mPFC. Strikingly, chronic treatment with the glutathione precursor N-acetylcysteine (NAC) from postnatal days 5–90 restored not only antioxidant-related mRNA expression and mitochondria numbers, but also myelin-related mRNA expression and mPFC-dependent cognitive dysfunction, while blood glutathione levels remained unaffected. The promyelinating effect of NAC was at least partly due to a positive effect on oligodendrocyte lineage progression. Together, our findings highlight that oxidative stress may contribute to cognitive symptoms in the APO-SUS rat model of SZ and encourage antioxidant therapy in early phases of SZ.
Late-life depression accentuates cognitive weaknesses in older adults with small vessel disease
Authors: Oberlin Lauren E. , Respino Matteo , Victoria Lindsay , Abreu Lila , Hoptman Matthew J. , Alexopoulos George S. , Gunning Faith M. 
Published: February 2021

AbstractNeuroimaging features of small vessel disease (SVD) are highly prevalent in older adulthood and associated with significant variability in clinical symptoms, yet the factors predicting these symptom disparities are poorly understood. We employed a novel metric of SVD, peak width of skeletonized mean diffusivity (PSMD), to elucidate the relationship of late-life depression (LLD) to the cognitive presentation of vascular pathology. A total of 109 older adults without a diagnosis of a neurocognitive disorder were enrolled in the study; 44 with major depressive disorder and 65 age-matched controls. Subjects completed neuropsychological testing and magnetic resonance imaging including FLAIR and diffusion tensor imaging sequences, from which white matter hyperintensity volume and diffusion metrics (fractional anisotropy, mean diffusivity, PSMD) were quantified. In hierarchical models, the relationship between vascular burden and cognitive performance varied as a function of diagnostic status, such that the negative association between PSMD and processing speed was significantly stronger in participants with LLD compared to controls. Greater PSMD also predicted poorer performance on delayed memory and executive function tasks specifically among those with LLD, while there were no associations between PSMD and task performance among controls. PSMD outperformed conventional SVD and diffusion markers in predicting cognitive performance and dysexecutive behaviors in participants with LLD. These data suggest that LLD may confer a vulnerability to the cognitive manifestations of white matter abnormalities in older adulthood. PSMD, a novel biomarker of diffuse microstructural changes in SVD, may be a more sensitive marker of subtle cognitive deficits stemming from vascular pathology in LLD.
Early diet in preterm infants and later cognition: 10-year follow-up of a randomized controlled trial
Authors: Embleton Nicholas D. , Wood Claire L. , Pearce Mark S. , Brunskill Greta , Grahame Victoria 
Published: February 2021

AbstractBackgroundAchieving adequate nutrition in preterm infants is challenging. The post-discharge period may be critical for influencing growth and cognitive outcomes. We studied the effects of post-discharge nutrition on childhood cognition.MethodsPreterm-born children were randomized at ~36 weeks corrected age (CGA) to either preterm formula (PTF) or term formula (TF) until 6 months, or PTF until 40 weeks CGA, then TF until 6 months (crossover group). Childhood cognition was assessed using the short form Wechsler Intelligence Scale for Children III, allowing computation of full-scale intelligence quotient (FSIQ) and four-factor index scores; verbal comprehension, freedom from distractibility (FDI), perceptual organization (POI), and processing speed (PSI).ResultsNinety-two children were recruited (mean 10.1 years). FSIQ did not differ by group. PTF-fed children had 10-point higher PSI (p = 0.03) compared to crossover. Faster weight gain from term to 12 weeks CGA was associated with 5-point higher FSIQ (p = 0.02) and four-point higher POI (p = 0.04). Infant head growth was positively associated with FSIQ (+3.8 points,p = 0.04) and FDI (+6 points,p = 0.003).ConclusionsWhile there is no long-term impact of post-discharge macronutrient enrichment on childhood cognition, greater weight and head growth in specific epochs is associated with better outcomes. Further studies are needed to determine optimal early diet in preterm infants.ImpactAchieving adequate nutrient intakes in preterm infants before and after hospital discharge is challenging.Nutrient intakes prior to discharge affect later cognitive and metabolic outcomes.Follow-up of a randomized controlled trial shows no cognitive benefit in later childhood from a more nutrient-dense formula compared to standard formula after hospital discharge.Growth in the first year of life is strongly correlated with childhood cognition and emphasizes the importance of nutrition in early life.
Contribution of horizontal gene transfer to the functionality of microbial biofilm on a macroalgae
Authors: Song Weizhi , Wemheuer Bernd , Steinberg Peter D , Marzinelli Ezequiel M , Thomas Torsten 
Published: February 2021

Abstract
Horizontal gene transfer (HGT) is thought to be an important driving force for microbial evolution and niche adaptation and has been show in vitro to occur frequently in biofilm communities. However, the extent to which HGT takes place and what functions are being transferred in more complex and natural biofilm systems remains largely unknown. To address this issue, we investigated here HGT and enrichment of gene functions in the biofilm community of the common kelp (macroalgae) Ecklonia radiata in comparison to microbial communities in the surrounding seawater. We found that HGTs in the macroalgal biofilms were dominated by transfers between bacterial members of the same class or order and frequently involved genes for nutrient transport, sugar and phlorotannin degradation as well as stress responses, all functions that would be considered beneficial for bacteria living in this particular niche. HGT did not appear to be driven by mobile gene elements, indicating rather an involvement of unspecific DNA uptake (e.g. natural transformation). There was also a low overlap between the gene functions subject to HGT and those enriched in the biofilm community in comparison to planktonic community members. This indicates that much of the functionality required for bacteria to live in an E. radiata biofilm might be derived from vertical or environmental transmissions of symbionts. This study enhances our understanding of the relative role of evolutionary and ecological processes in driving community assembly and genomic diversity of biofilm communities.
Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs
Authors: Maire Justin , Girvan Sam K , Barkla Sophie E , Perez-Gonzalez Alexis , Suggett David J , Blackall Linda L , van Oppen Madeleine J H 
Published: February 2021

Abstract
Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian–microorganism interactions are widely studied, Symbiodiniaceae–bacteria interactions are only just beginning to receive attention. Here, we describe the localization and composition of the bacterial communities associated with cultures of 11 Symbiodiniaceae strains from nine species and six genera. Three-dimensional confocal laser scanning and electron microscopy revealed bacteria are present inside the Symbiodiniaceae cells as well as closely associated with their external cell surface. Bacterial pure cultures and 16S rRNA gene metabarcoding from Symbiodiniaceae cultures highlighted distinct and highly diverse bacterial communities occur intracellularly, closely associated with the Symbiodiniaceae outer cell surface and loosely associated (i.e., in the surrounding culture media). The intracellular bacteria are highly conserved across Symbiodiniaceae species, suggesting they may be involved in Symbiodiniaceae physiology. Our findings provide unique new insights into the biology of Symbiodiniaceae.
Spatial patterns in phage-Rhizobium coevolutionary interactions across regions of common bean domestication
Authors: Van Cauwenberghe Jannick , Santamaría Rosa I , Bustos Patricia , Juárez Soledad , Ducci Maria Antonella , Figueroa Fleming Trinidad , Etcheverry Angela Virginia , González Víctor 
Published: February 2021

Abstract
Bacteriophages play significant roles in the composition, diversity, and evolution of bacterial communities. Despite their importance, it remains unclear how phage diversity and phage-host interactions are spatially structured. Local adaptation may play a key role. Nitrogen-fixing symbiotic bacteria, known as rhizobia, have been shown to locally adapt to domesticated common bean at its Mesoamerican and Andean sites of origin. This may affect phage-rhizobium interactions. However, knowledge about the diversity and coevolution of phages with their respective Rhizobium populations is lacking. Here, through the study of four phage-Rhizobium communities in Mexico and Argentina, we show that both phage and host diversity is spatially structured. Cross-infection experiments demonstrated that phage infection rates were higher overall in sympatric rhizobia than in allopatric rhizobia except for one Argentinean community, indicating phage local adaptation and host maladaptation. Phage-host interactions were shaped by the genetic identity and geographic origin of both the phage and the host. The phages ranged from specialists to generalists, revealing a nested network of interactions. Our results suggest a key role of local adaptation to resident host bacterial communities in shaping the phage genetic and phenotypic composition, following a similar spatial pattern of diversity and coevolution to that in the host.
p53 dynamics vary between tissues and are linked with radiation sensitivity
Authors: Stewart-Ornstein Jacob , Iwamoto Yoshiko , Miller Miles A. , Prytyskach Mark A. , Ferretti Stephane , Holzer Philipp , Kallen Joerg , Furet Pascal , Jambhekar Ashwini , Forrester William C. , Weissleder Ralph , Lahav Galit 
Published: February 2021

AbstractRadiation sensitivity varies greatly between tissues. The transcription factor p53 mediates the response to radiation; however, the abundance of p53 protein does not correlate well with the extent of radiosensitivity across tissues. Given recent studies showing that the temporal dynamics of p53 influence the fate of cultured cells in response to irradiation, we set out to determine the dynamic behavior of p53 and its impact on radiation sensitivity in vivo. We find that radiosensitive tissues show prolonged p53 signaling after radiation, while more resistant tissues show transient p53 activation. Sustaining p53 using a small molecule (NMI801) that inhibits Mdm2, a negative regulator of p53, reduced viability in cell culture and suppressed tumor growth. Our work proposes a mechanism for the control of radiation sensitivity and suggests tools to alter the dynamics of p53 to enhance tumor clearance. Similar approaches can be used to enhance killing of cancer cells or reduce toxicity in normal tissues following genotoxic therapies.
Colloidal CdSe nanocrystals are inherently defective
Authors: Goldzak Tamar , McIsaac Alexandra R. , Van Voorhis Troy 
Published: February 2021

AbstractColloidal CdSe nanocrystals (NCs) have shown promise in applications ranging from LED displays to medical imaging. Their unique photophysics depend sensitively on the presence or absence of surface defects. Using simulations, we show that CdSe NCs are inherently defective; even for stoichiometric NCs with perfect ligand passivation and no vacancies or defects, we still observe that the low energy spectrum is dominated by dark, surface-associated excitations, which are more numerous in larger NCs. Surface structure analysis shows that the majority of these states involve holes that are localized on two-coordinate Se atoms. As chalcogenide atoms are not passivated by any Lewis base ligand, varying the ligand should not dramatically change the number of dark states, which we confirm by simulating three passivation schemes. Our results have significant implications for understanding CdSe NC photophysics, and suggest that photochemistry and short-range photoinduced charge transfer should be much more facile than previously anticipated.
Lasting antibody and T cell responses to SARS-CoV-2 in COVID-19 patients three months after infection
Authors: Jiang Xiao-Lin , Wang Guo-Lin , Zhao Xiang-Na , Yan Fei-Hu , Yao Lin , Kou Zeng-Qiang , Ji Sheng-Xiang , Zhang Xiao-Li , Li Cun-Bao , Duan Li-Jun , Li Yan , Zhang Yu-Wen , Duan Qing , Wang Tie-Cheng , Li En-Tao , Wei Xiao , Wang Qing-Yang , Wang Xue-Feng , Sun Wei-Yang , Gao Yu-Wei , Kang Dian-Min , Zhang Ji-Yan , Ma Mai-Juan 
Published: February 2021

AbstractThe dynamics, duration, and nature of immunity produced during SARS-CoV-2 infection are still unclear. Here, we longitudinally measured virus-neutralising antibody, specific antibodies against the spike (S) protein, receptor-binding domain (RBD), and the nucleoprotein (N) of SARS-CoV-2, as well as T cell responses, in 25 SARS-CoV-2-infected patients up to 121 days post-symptom onset (PSO). All patients seroconvert for IgG against N, S, or RBD, as well as IgM against RBD, and produce neutralising antibodies (NAb) by 14 days PSO, with the peak levels attained by 15–30 days PSO. Anti-SARS-CoV-2 IgG and NAb remain detectable and relatively stable 3–4 months PSO, whereas IgM antibody rapidly decay. Approximately 65% of patients have detectable SARS-CoV-2-specific CD4+ or CD8+ T cell responses 3–4 months PSO. Our results thus provide critical evidence that IgG, NAb, and T cell responses persist in the majority of patients for at least 3–4 months after infection.
Melting and density of MgSiO3 determined by shock compression of bridgmanite to 1254GPa
Authors: Fei Yingwei , Seagle Christopher T. , Townsend Joshua P. , McCoy Chad A. , Boujibar Asmaa , Driscoll Peter , Shulenburger Luke , Furnish Michael D. 
Published: February 2021

AbstractThe essential data for interior and thermal evolution models of the Earth and super-Earths are the density and melting of mantle silicate under extreme conditions. Here, we report an unprecedently high melting temperature of MgSiO3at 500 GPa by direct shockwave loading of pre-synthesized dense MgSiO3(bridgmanite) using the Z Pulsed Power Facility. We also present the first high-precision density data of crystalline MgSiO3to 422 GPa and 7200 K and of silicate melt to 1254 GPa. The experimental density measurements support our density functional theory based molecular dynamics calculations, providing benchmarks for theoretical calculations under extreme conditions. The excellent agreement between experiment and theory provides a reliable reference density profile for super-Earth mantles. Furthermore, the observed upper bound of melting temperature, 9430 K at 500 GPa, provides a critical constraint on the accretion energy required to melt the mantle and the prospect of driving a dynamo in massive rocky planets.