bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Density of Calretinin Striatal Interneurons Is Decreased in 6-OHDA-Lesioned Mice
Authors: Petryszyn Sarah , Saidi Lydia , Gagnon Dave , Parent André , Parent Martin 
Published: April 2021

Abstract
Interneurons play a significant role in the functional organization of the striatum and some of them display marked plastic changes in dopamine-depleted conditions. Here, we applied immunohistochemistry on brain sections from 6-hydroxydopamine (6-OHDA) mouse model of Parkinson’s disease and sham animals to characterize the regional distribution and the morphological and neurochemical changes of striatal interneurons expressing the calcium binding protein calretinin (CR). Two morphological subtypes of calretinin-immunostained (CR+) interneurons referred respectively as small and medium-sized CR+ interneurons were detected in 6-OHDA and sham-lesioned animals. The small cells (9-12 µm) prevail in the anterior and dorsal striatal regions; they stain intensely for CR and display a single slightly varicose and moderately arborized process. The medium-sized CR+ interneurons (15-20 µm) are slightly more numerous than the small CR+ cells and rather uniformly distributed within the striatum; they stain weakly for CR and display 2-3 long, slightly varicose and poorly branched dendrites. The density of medium CR+ interneurons is significantly decreased in the dopamine-depleted striatum (158 ± 15 neurons/mm3), when compared to sham animals (370 ± 41 neurons/mm3), whereas that of the small-sized CR+ interneurons is unchanged (174 ± 46 neurons/mm3 in 6-OHDA-lesioned striatum and 164 ± 22 neurons/mm3 in sham-lesioned striatum). The nucleus accumbens is populated only by medium-sized CR+ interneurons, which are distributed equally among the core and shell compartments and whose density is unaltered after dopamine denervation. Our results provide the first evidence that the medium-sized striatal interneurons expressing low level of CR are specifically targeted by dopamine denervation, while the small and intensely immunoreactive CR+ cells remain unaffected. These findings suggest that high expression of the calcium binding protein CR might protect striatal interneurons against an increase in intracellular calcium level that is believed to arise from altered glutamate corticostriatal transmission in Parkinson’s disease.
CT Image Analysis Using Grayscale Statistics to Categorise Severity of Lung Abnormalities of COVID-19 Patients
Authors: Ghashghaei Sara , Wood David A. , Sadatshojaei Erfan , Jalilpoor Mansooreh 
Published: April 2021

Abstract
Grayscale image attributes from 456 images extracted from CT scan slices of 53 patients (49 with COVID-19 and 4 without) are used to establish a visual scale of severity of lung abnormalities (five classes: 0 to 4). The complex trends of these easy-to-derive image attributes can be used graphically to discern the visual scale of lung abnormalities in broad terms. With the aid of machine learning algorithms, the visual classes can be distinguished with close to 95% accuracy using combinations of selected grayscale attributes. Confusion matrices reveal that the best-performing machine learning models are able to distinguish more accurately between certain classes than visual inspection of CT images by experts. The adaboost, decision tree and random forest models confused on average less than 25 of the 456 CT-scan image extracts evaluated between the visual classes of lung abnormalities.
The 5-Factor Modified Frailty Index Predicts Complications and Worsening After Meningioma Surgery in Non-elderly Rather Than Elderly Patients: A Nationwide Registry Study
Authors: Ikawa Fusao , Michihata Nobuaki , Oya Soichi , Hidaka Toshikazu , Matsuda Shingo , Ozono Iori , Ohata Kenji , Saito Kiyoshi , Yoshida Kazunari , Fushimi Kiyohide , Yasunaga Hideo , Tominaga Teiji , Kurisu Kaoru 
Published: April 2021

Abstract
The simplified 5-factor modified frailty index (mFI-5) is a useful indicator of outcome for patients undergoing surgeries and considered as an important risk factor in elderly patients. However, its usefulness has not been validated based on age groups. We aimed to investigate the risk factors including the mFI-5 across age groups for complications and worse outcomes in meningioma surgery using data obtained from the nationwide database in Japan. We extracted data from the nationwide registry database in Japan between 2010 and 2015. Age (< 65, 65–74, and ≥ 75 years), sex, Barthel Index (BI), mFI-5 scores, and complications were evaluated. Multivariate logistic regression analyses identified risk factors across all age groups for worsening BI scores and complications after surgery. In 8,138 included cases, an mFI-5 score ≥ 2 items was a significant risk factor for worsening BI scores in patients aged < 65 years (odds ratio: 2.00; 95% confidence interval: 1.31-3.06), but not in patients aged 65-74 years and those aged ≥ 75 years. Similar results were noted for complications in patients aged < 65 years (2.40; 1.67–3.44), but not in patients aged 65-74 years and those aged ≥ 75 years. In conclusion, the mFI-5 scores can predict the risk of worsening outcome and complications in non-elderly patients aged < 65 years rather than in elderly patients aged ≥ 65 years. In meningioma surgeries, care must be taken when making decisions using the mFI-5 scores based on the patients’ age.Trial RegistrationName: Study on treatment method, age group, complications, and outcome of meningiomas and hemangioblastomas using DPC, URL: http://www.umin.ac.jp/ctr/index-j.htmID: UMIN000038486, No.: R000043856
Growth and physio-biochemical responses of Azolla filiculoides subjected to short span of phenol exposure
Authors: Paul Bittu , Sarkar Ashis , Roy Swarnendu 
Published: April 2021

Abstract
Phenol and phenolic compounds are one of the major water pollutants increasing at an alarming rate due to uncontrolled anthropogenic activities. Aquatic plants are in the frontline to face the toxic effects of these pollutants. The present study attempts to study the effect of external phenols on a floating fern - Azolla filiculoides. In this context, the effect of four concentrations of crystalline phenol (0.5, 1, 2 and 3 mM) along with a control set (0 mM) were used to evaluate the changes in the responses of A. filiculoides plants in terms of the major morphological, physiological and biochemical markers, after being exposed for 3 and 6 days. In this study, relative growth rate, relative frond number, relative frond surface area, relative water content, chlorophyll a and carotenoids decreased significantly both with increasing phenol concentration and also with the duration of treatment. Frond necrotic area, electrolyte leakage and malondialdehyde content increased significantly which demonstrated the negative effects of phenol. However, increase in proline content, total sugars, activities of antioxidative enzymes like superoxide dismutase, catalase and peroxidase indicated the presence of an inherent tolerance mechanism in this plant. Accumulation of H2O2 also supported by the in situ localization of H2O2 along with the changes in growth parameters and physio-biochemical indicators demonstrated that the extent of phenol toxicity increased with the increasing concentrations of phenol. The overall findings also suggest that the A. filiculoides plants can maintain their growth and physiology up to a concentration of 1 mM phenol.
An Exploration of Changes in Plantar Pressure Distributions During Walking With Standalone and Supported Lateral Wedge Insole Designs&nbsp;
Authors: Tse Calvin , Ryan Michael , Dien Jason , Scott Alex , Hunt Michael 
Published: April 2021

Abstract
Background: Lateral wedge insoles (LWI), standalone or with medial arch support (supported-LWI), have been thoroughly investigated for their effects on modifying gait biomechanics for people with knee osteoarthritis. However, plantar pressure distribution between these insole types has not been investigated and could provide insight towards insole prescription with concomitant foot symptoms taken into consideration.Methods: In a sample of healthy individuals (n = 40), in-shoe plantar pressure was measured during walking with LWI, with or without medial arch support (variable- and uniform-stiffness designs), and a flat control insole condition. Pressure data from the plantar surface of the foot was divided into seven regions: medial/lateral rearfoot, midfoot, medial/central/lateral forefoot, hallux. Plantar pressure outcomes assessed were the medial-lateral pressure index (MLPI) for the whole foot, and the peak pressure, pressure-time integral (PTI), and contact area in each plantar region. Comfort in each insole condition was rated as a change relative to the flat control insole condition. Repeated-measures analyses of variance were calculated to compare the plantar pressure outcomes between insole conditions. Results: Regionally, medial rearfoot and forefoot pressure were reduced by all wedged insoles, with the variable-stiffness supported-wedge showing greater reductions than the standalone wedge. Lateral rearfoot and forefoot pressure were reduced by both supported-LWI, but unchanged by the standalone wedge. In the midfoot, the standalone wedge maintained pressure but reduced regional contact area, while both supported-LWI increased midfoot pressure and contact area. All LWI increased the MLPI, indicating a lateral shift in plantar pressure distribution throughout the weightbearing phase of gait. Comfort ratings were not significantly different between insole conditions. ﻿Conclusions: Regional differences in plantar pressure may help determine an appropriate lateral wedge insole variation to avoid exacerbation of concomitant foot symptoms by minimizing pressure in symptomatic regions. Lateral shifts in plantar pressure distribution were observed in all laterally wedged conditions, including one supported-LWI that was previously shown to be biomechanically ineffective for modifying knee joint load distribution. Thus, shifts in foot centre of pressure may not be a primary mechanism by which LWI can modify knee joint load distribution for people with knee osteoarthritis.
Clinical and molecular epidemic characterization of rotavirus infection among children under five years old in Shandong province, China.
Authors: Dong Shixiao , Huang Deyu , Wang Zheng , Zhang Guanyou , Zhang Fengjuan , Sai Lintao 
Published: April 2021

Abstract
Rotavirus was the an important causative agent of acute gastroenteritis in children. In China, rotavirus was positive in approximately 30% of the diarrhea children and become a serious public problem. This study was carried out to investigate the clinical and molecular epidemic characterization of rotavirus infection among children under 5 years old with acute diarrhea in Shandong province, China. From July 2017 to June 2018, a total of 1211 fecal specimens were detected and the prevalence of rotavirus infection was 32.12%. The mean age of positive children was 12.2 ± 10.9 months and the highest infection rate was observed in children aged 7–12 months with a rate of 41.64%. G9P[8] (76.61%) was the most prevalent combinations followed by G2P[4] (7.20%), G3P[8] (3.60%) and G9P[4] (2.06%). In addition to diarrhea, vomiting, fever and dehydration were the most common accompanied symptoms. In general, there was no significant difference in clinical manifestations among different age groups. However, the clinical manifestations between vaccinated and unvaccinated children were significantly different. Vaccinated children showed lower incidence and frequency of vomiting, lower incidence and degree of dehydration, lower incidence of severe cases than unvaccinated children. The findings suggested necessary to continue rotavirus strains surveillance in order to monitor the change of prevalent genotype. Moreover, introducing vaccine into national immunization program to prevent and control rotavirus infections is needed in China.
The Immunolocalization of Cadherins and Beta-Catenin in the Cervix and Vagina of Cycling Cows
Authors: Liman Narin , Sağsöz Hakan 
Published: April 2021

Abstract
The adherens junction (AJ) maintains the structural integrity and barrier function of the epithelial cell layers. AJs also play a key role in a variety of biological and pathological processes, from morphogenesis to tumor progression. AJs perform these functions through the cadherin-catenin adhesion complex. In this study, we investigated the presence, cell-specific localization, and temporal distribution of AJ components such as cadherins and beta-catenin in the cow cervix and vagina during the oestrous cycle using immunohistochemistry. The cow genitalia (n = 30) were collected from an abattoir and the cervix and vagina were categorized into the follicular and luteal groups based on cyclicity. Results demonstrated constitutive expression of beta-catenin and placental (P)- and epithelial (E)-cadherins, but not neural (N)-cadherin, in ciliated and non-ciliated columnar cervical cells, the luminal, parabasal, intermediate, and basal layers of the stratified vaginal epithelium of the bovine cervix and vagina throughout the oestrous cycle. The honeycomb-like membrane staining pattern for selected junctional molecules was observed in the epithelial cells. While there were no noticeable variations in the immunostaining intensity of P- and E-cadherin and beta-catenin proteins in the cervical and vaginal epithelium between the oestrous phases, the immunolocalization patterns altered by structural changes that occurred in response to oestrogen and progesterone hormone levels during the oestrous cycle. These results may indicate that P- and E-cadherin and beta-catenin participate in maintaining the integrity and barrier function of the cervical and vaginal epithelium throughout the oestrous cycle, thus helping to maintain the sterility of the uterine cavity.
Title: Initial results of reoperative bariatric surgery: a retrospective feasibility and safety study at a low-volume unit
Authors: Wu Sheng Mao , Lo Hung Chieh 
Published: April 2021

Abstract
Background This study assessed the feasibility and results of revisional bariatric surgery at a low-volume unit.Methods This retrospective study was conducted from January 2017 to August 2020; the revision group comprised patients treated for weight regain (WR, n = 6), insufficient weight loss (IWL, n =3), and various complications (n=6). Clinical characteristics and 30-day outcomes were assessed and compared with those of primary bariatric procedures (control, n=173). Results A total of 8.0% (15/188) of patients underwent revisional procedures and tended to be elderly (40.1 vs. 38.2 years), be female (73.3% vs. 54.9%), and have a significantly lower body mass index (33.1 vs. 39.9 kg/m2) and fewer comorbidities than controls. Gastric bypass was the most prevalent revisional procedure. A significantly longer operative duration (155 and 96 min; p < 0.001), longer length of stay (3.7 and 2.4 days) and higher 30-day complication rate (20.0% vs. 4.6%) were found in the revision group, including one case of leakage and another of jejunojejunostomy obstruction. There were no cases of conversion to open surgery or mortality. A total of 5/6 WR patients achieved excessive weight loss >50%, versus only one IWL patient who reached this goal. Complications, including marginal ulcer, fistula and post-sleeve gastrectomy stenosis, were alleviated after revision.Conclusion Revisional surgery appears to be feasible and effective in a low-volume practice in patients with WR and complications after primary bariatric procedures; however, the benefits should be weighed against the risks. More robust evidence is required to support ongoing practice.
Comparison of Hidden Blood Loss Between Cortical Bone Trajectory Screw Fixation and Traditional Pedicle Screw Fixation
Authors: Hu Xinli , Wu Chenyu , Xu Cong , Zhou Kailiang , Li Xiaobin , Jin Chenqiang , Chen Yanlin , Zhang Haojie , Su Haohan , Ni Wenfei 
Published: April 2021

Abstract
Objective: This research aimed at examining the volume of hidden blood loss (HBL) in lumbar fusion surgery with two kinds of screw implanting techniques and evaluating HBL-related factors in the patient population receiving lumbar fusion surgeryMethods: A retrospective study was conducted targeting 104 patients with lumbar degenerative diseases and treated with TLIF from January 2017 to December 2018, and the CBT (cortical bone trajectory) technique and conventional PS (pedicle screw) technique were applied to 45 and 59 patients, respectively. The collected data covered patients’ weight, height, BMI index, and operation time. It was followed by the recording of preoperative and postoperative hematocrit (HCT) of patients, based on which the blood loss was calculated by Gross’s formula. Results: The CBT and PS groups presented no significant difference in demographic characteristics (p > 0. 05), but the average HBL of 280±227 mL and 298±232 mL as well as the average TBL of 603±232 mL and 728±321 mL, respectively. It indicated the difference of the two groups in TBL, but no significant difference in HBL. In addition, an association of HBL of two screw techniques with operation time, concomitant disease and age was found from the data analysis.Conclusion: Large total blood loss incurred in lumbar fusion surgery, specifically with a lower value by the CBT technique than by the PS technique. Nonetheless, HBL occupied a large part of the total blood loss and it was identified in both groups. Thus, a good understanding of HBL will improve postoperative rehabilitation and guarantee patients’ safety.
Catastrophic Failure and Metallosis of the Acetabular Component in Total Hip Arthroplasty
Authors: OZAN FIRAT , Kahraman Murat , Baktır Ali , Gençer Kürşat 
Published: April 2021

Abstract
Background: To evaluate the clinical features and results of the revision total hip arthroplasties (THA) in patients with catastrophic failures and metallosis. Methods: Fifteen hips of 14 patients with catastrophic failure and metallosis in hip arthroplasties were evaluated. They were followed for at least 4.2 years after the revision THA. Clinical evaluation was performed using Harris hip score. Osteolysis, loosening or presence of metallosis was evaluated with standard radiographs. Metallosis was evaluated intraoperatively according to the metallosis severity classification. Results: The mean time from failure until revision surgery was 9.4 years. It was observed that in the primary THA, metal-on-ceramic (MoC), ceramic-on-ceramic (CoC) and metal-on-conventional polyethylene (MoCPE) bearings were used in 1, 3 and 11 hips, respectively. Grade III metallosis was observed in all patients during revision surgeries. The mean Harris hip score increased from 55 points before revision THA to 75 points at the final follow-up. In revision arthroplasty, MoCPE and CoC bearings were used in 13 and 2 hips, respectively. The femoral stem was replaced in 5 hips. All acetabular cups, except that of one hip, were revised. Conclusions: Revisions of THAs with catastrophic failures and metallosis are quite challenging. Routine follow-up of arthroplasty patients is beneficial to examine for osteolysis, loosening, and asymmetric wear.
Peritoneal inclusion cyst in a patient with glycogen storage disease type Ib and Crohn’s like disease
Authors: Grünert Sarah Catharina , Teufel-Schäfer Ulrike , Berg Sebastian , Schumann Anke , Grimm Juliane , Rosenbaum-Fabian Stefanie , Spiekerkoetter Ute 
Published: April 2021

Abstract
Background

Glycogen storage disease type Ib (GSD Ib) due to biallelic mutations in SLC37A4 is often associated with inflammatory bowel disease. Peritoneal inclusion cysts (PICs) are variable sized, fluid-filled, mesothelial-lined cysts that usually occur in premenopausal woman. Risk factors for the development of PICS comprise prior abdominal surgeries and inflammatory diseases.
Results

We herein report on a female patient with GSD Ib and Crohn’s like disease who underwent recurrent abdominal surgery due to suspected intraabdominal abscesses. At the age of 36, abdominal pain and an increase in the abdominal girth was observed. MRI revealed a large PIC. Due to multiple previous complications conservative management was chosen, and the cyst remained stable in size during a 1-year follow-up.
Conclusions

PIC should be included in the differential diagnosis of abdominal cystic masses especially in premenopausal women with either IBD or previous abdominal surgery. Radiologist, gynecologist, and gastroenterologist should be aware of this rare condition to prevent inappropriate and aggressive treatments.
Early Treatment with Integrase Inhibitor Achieves a Functional Cure for Pediatric SIV Infection
Authors: Wang Xiaolei , Vincent Eunice , Shao Jiasheng , Lu Hong , Siddiqui Summer , Blair Robert , Wu Xueling , Doyle-Meyers Lara A , Russell-Lodrigue Kasi E. , Ziani Widade , Bohm Rudolf P. , Veazey Ronald S. , Xu Huanbin 
Published: April 2021

Abstract
Neonatal HIV infection causes rapid disease progression to end-stage of AIDS if untreated. To date, there are no reported cases of a genuine functional cure in Pediatric AIDS ClinicalTrial Group lacking integrase inhibitor regimen, even when initiated early. In this study, three infant rhesus macaques born on the same day were intravenously infected with SIV on the day of birth and received ART containing tenofovir, FTC, and dolutegravir (DTG) initiated 3 days post infection and continued daily for 9 months. The results showed early ART combination effectively suppressed viral replication to undetectable viremia throughout the treatment course. Remarkably, two of three infants showed complete virologic remission and intestinal CD4+ T cell preservation throughout the period of studyafter analytical treatment interruption (ATI), as well as undetectable cell-associated proviral DNA in PBMCs, axillary lymph nodes (LNs), and rectal biopsies. However, viral rebound was observed in one of the three infants at two months post ATI. Notably, very low levels of integrated proviral SIV DNA were detected in the lymph node of this animal under cART. Sustained virologic remission was achieved in the other two infants despite absence of protective MHC alleles or neutralizing antibody responses. These findings suggest that early integrase inhibitor-based ART, initiated before irreversible proviral reservoir seeding, can completely suppress viral replication and effectively block new viral genome integration, providing a proof of concept that HIV functional cure and possibly a cure is possible, at least in a proportion of infants postnatally infected with HIV.
Design of SiN waveguides integrated with graphene oxide films for nonlinear optics
Authors: Qu Yang , Wu Jiayang , Zhang Yuning , Jia Linnan , Liang Yao , Jia Baohua , Moss David 
Published: April 2021

Abstract
We theoretically investigate and optimize four-wave mixing (FWM) in silicon nitride (SiN) waveguides integrated with two-dimensional (2D) layered graphene oxide (GO) films. Based on extensive previous measurements of the material parameters of the GO films, we perform detailed analysis for the influence of device parameters including waveguide geometry, GO film thickness, length, and coating position on the FWM conversion efficiency (CE) and conversion bandwidth (CB). The influence of dispersion and photo-thermal changes in the GO films is also discussed. Owing to the strong mode overlap between the SiN waveguides and the highly nonlinear GO films, FWM in the hybrid waveguides can be significantly enhanced. We obtain good agreement with previous experimental results and show that by optimizing the device parameters to balance the trade-off between Kerr nonlinearity and loss, the FWM CE can be improved by as much as ~20.7 dB and the FWM CB can be increased by ~4.4 folds, relative to the uncoated waveguides. These results highlight the significantly enhanced FWM performance that can be achieved in SiN waveguides by integrating 2D layered GO films.