bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Impact of Pneumothorax in Patients With Pneumocystis Jirovecii Pneumonia and Respiratory Failure in An HIV-Negative Cohort
Authors: Choi Ji Soo , Kwak Se Hyun , Kim Min Chul , Seol Chang Hwan , Kim Sung Ryeol , Park Byung Hoon , Lee Eun Hye , Yong Seung Hyun , Leem Ah Young , Kim Song Yee , Lee Sang Hoon , Chung Kyungsoo , Kim Eun Young , Jung Ji Ye , Kang Young Ae , Park Moo Suk , Kim Young Sam , Lee Su Hwan 
Published: October 2021

Abstract
Background: Pneumocystis jirovecii pneumonia (PCP) with acute respiratory failure can result in development of pneumothorax during treatment. This study aimed to identify the incidence and related factors of pneumothorax in patients with PCP and acute respiratory failure and to analyze their prognosis.Methods: We retrospectively reviewed the occurrence of pneumothorax, including clinical characteristics and results of other examinations, in 119 non-human immunodeficiency virus patients with PCP and respiratory failure requiring mechanical ventilator treatment in a medical intensive care unit (ICU) at a tertiary-care center between July 2016 and April 2019.Results: The 28-day survival rate was 62.2% (N=74). Twenty-two patients (18.5%) developed pneumothorax during ventilator treatment, with 45 (37.8%) eventually requiring a tracheostomy due to weaning failure. Cytomegalovirus co-infection (odds ratio 13.9; p=0.013) was related with occurrence of pneumothorax. Of 74 patients with 28-day survival data, 15 (20.3%) developed pneumothorax. In those patients, five survived (p=0.048) and only two were successfully weaned from mechanical ventilation (p=0.037).Conclusions: Patients with PCP and acute respiratory failure who developed pneumothorax did not have increased 28-day mortality, however pneumothorax increased in patient with cytomegalovirus co-infection, pneumothorax might have difficulty in and prolonged weaning from mechanical ventilators, which clinicians should be aware of when planning treatment for such patients.
Bioactivity of Recombinant Humanized Monoclonal Antibody Against HER2 and Its Mechanism of Action on Ovarian Cancer
Authors: Zeng Jinfeng , Zhang Rui , Guan Rui 
Published: October 2021

Abstract
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) protein is overexpressed on the surface of various epithelial ovarian cancer tissues, which mediates the proliferation, differentiation, metastasis and signal transduction of tumor cells and is a potential cancer therapeutic target.METHODS: In this paper, the recombinant anti-HER2 humanized IgG1 monoclonal antibody (rhHer2-mAb) was expressed in HEK293F cells by constructing mammalian cell expression vector and optimizing transfection conditions. The antibody was purified by rProtein A affinity chromatography, and its mediated antibody dependent cytotoxicity (ADCC) was identified by lactate dehydrogenase(LDH) lactate dehydrogenase release assay. The anti-tumor activity of rhHer2-mAb was evaluated in NOD/SCID mice. RESULTS: The expression of rhHer2-mAb in HEK293F cells was at the highest level (100.5 mg/L) when the ratios of DNA/PEI and light chain/heavy chain was 1:4 and 1:2, respectively. The IC50 on ADCC of antibodies against SK-OV-3, OVCAR-3 and A-2780 cells were 12.36, 5.43 and 102.90ng/ml, respectively. Animal experiments in mice showed that rhHer2-mAb could effectively inhibit the growth of SK-OV-3 tumor at the dose of 10 mg/kg.CONCLUSIONS: The recombinant monoclonal antibody was obtained by transient gene expression technology and its bioactivity was studied in vitro and in vivo , providing a novel insight for the development and production of future biotechnology-based drugs using transient gene expression technology of HEK293F.
A Rapid Antibody Screening Haemagglutination Test for Predicting Immunity to Sars CoV-2 Variants of Concern
Authors: Ertesvåg Nina Urke , Xiao Julie , Zhou Fan , Ljostveit Sonja , Sandnes Helene , Jalloh Sarah Lartey , Saevik Marianne , Hansen Lena , Madsen Anders , Mohn Kristin , Fjelltveit Elisabeth , Olofsson Jan Stefan , Tan Tiong Kit , Rijal Pramila , Schimanski Lisa , Øyen Sir , Dunachie Susanna , James William , Harding Adam , Harvala Heli , Jämsén Anni , Roberts David , Zambon Maria , group PHE Virology , Broksatd Karl , group Oxford Collaborative , Townsend Alain , Langeland Nina ,   Bergen COVID-19 Research Group, Cox Rebecca 
Published: October 2021

Abstract

Evaluation of susceptibility to emerging SARS-CoV-2 variants of concern (VOC) requires rapid screening tests for neutralising antibodies which provide protection. We developed a receptor-binding domain specific hemagglutination test (HAT) which correlated with neutralising antibodies (R=0.74-0.82) in two independent cohorts from 798 convalescents. Home-dwelling older individuals (80-99 years, n=89) had significantly lower antibodies after one dose of BNT162b2 vaccine than younger adult vaccinees (n=310) and naturally infected individuals (n=307). The second vaccine dose boosted and broadened the antibody repertoire to VOC in naïve but not previously infected, older and younger adults. >75% of older adults responded after two vaccinations to alpha and delta, but only 59-62% to beta and gamma, compared to 96-97% of younger vaccinees and 68-76% of infected individuals. Overall, the HAT provides a surrogate marker for neutralising antibodies, could be used as a simple inexpensive, rapid test, rapidly adaptable to emerging VOC for large-scale evaluation of potentially diminishing vaccine effectiveness.
Application of An Exponential Mathematical Law of Cardiac Dynamics In 16 Hours
Authors: Velásquez Javier Oswaldo Rodríguez , Herrera Sandra Catalina Correra , Gómez Yesica Tatiana Beltrán , Rojas Jorge Gómez , Bohórquez Signed Esperanza Prieto , Correa Dharma Rodríguez , Rodríguez Sefirot , Guardiola Guillermo Trout , Cruz Ediltrudis Ramos de la , Guezguan Alexander 
Published: October 2021

Abstract
Introduction and objectives: nonlinear dynamics and fractal geometry have allowed the advent of an exponential mathematical law applicable to diagnose cardiac dynamics in 21 hours, however, it would be beneficial to reduce the time required to diagnose cardiac dynamics with this method in critical scenarios, in order to detect earlier complications that may require medical attention. The objective of this research is to confirm the clinical applicability of the mathematical law in 16 hours, with a comparative study against the Gold Standard. Methods: There were taken 450 electrocardiographic records of healthy patients and with cardiac diseases. A physical-mathematical diagnosis was applied to study cardiac dynamics, which consists of generating cardiac chaotic attractors based on the sequence of heart rate values during 16 hours, which were then measured with two overlapping grids according to the Box-Counting method to quantify the spatial occupation and the fractal dimension of each cardiac dynamic, with its respective statistical validation. Results: The occupation spaces of normal dynamics calculated in 16 hours were compatible with previous parameters established, evidencing the precision of the methodology to differentiate normality from abnormality. Sensitivity and specificity values of 100% were found, as well as a Kappa coefficient of 1. Conclusions: it was possible to establish differences between cardiac dynamics for 16 hours, suggesting that this method could be clinically applicable to analyze and diagnose cardiac dynamics in real time.
NLRP3 is a Novel Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma and Skin Cutaneous Melanoma
Authors: Yuan Chenxi , Wang Qingwei , Dai Xueting , Song Yipeng , Yu Jinming 
Published: October 2021

Abstract
Background: Lung adenocarcinoma (LUAD) and skin cutaneous melanoma (SKCM) are common tumors around the world. However, the prognosis in advanced patients is poor. Because NLRP3 was not extensively studied in cancers, so that we aimed to identify the impact of NLRP3 on LUAD and SKCM through bioinformatics analyses. Methods: TCGA and TIMER database were utilized in this study. We compared the expression of NLRP3 in different cancers and evaluated its influence on survival of LUAD and SKCM patients. The correlations between clinical information and NLRP3 expression were analyzed using logistic regression. Clinicopathologic characteristics associated with overall survival in were analyzed by Cox regression. In addition, we explored the correlation between NLRP3 and immune infiltrates. GSEA and co-expressed gene with NLRP3 were also done in this study. Results: NLRP3 expressed disparately in tumor tissues and normal tissues. Cox regression analysis indicated that up-regulated NLRP3 was an independent prognostic factor for good prognosis in LUAD and SKCM. Logistic regression analysis showed increased NLRP3 expression was significantly correlated with favorable clinicopathologic parameters such as no lymph node invasion and no distant metastasis. Specifically, a positive correlation between increased NLRP3 expression and immune infiltrating level of various immune cells was observed. Conclusion: Together with all these findings, increased NLRP3 expression correlates with favorable prognosis and increased proportion of immune cells in LUAD and SKCM. These conclusions indicate that NLRP3 can serve as a potential biomarker for evaluating prognosis and immune infiltration level.
3D Mapping of Intra-articular Calcaneal Fractures
Authors: Shi Guang , Zhan Hongrui , Liu Wei , Zhang Tao , Lin Zhao , Liao Xun , Cai Xiyu 
Published: October 2021

Abstract
Background: The management of intra-articular calcaneal fractures (ICFs) still faces a challenge. Available research about the anatomic patterns of ICFs is lacking. We aimed to define the pattern of ICFs by a three-dimensional (3D) mapping and determine whether there are consistent fracture patterns and comminution zones. Methods: 67 patients of ICFs with available computed tomographic (CT) scans were identified. The calcaneal fractures fragments on CT were multiplanar reconstructed and virtually reduced. 3D heat mapping was subsequently created by graphically superimposing all fracture lines onto a standard calcaneal template. Mapping of fracture lines and comminution zones in both the axial and sagittal planes were performed. Results: The cohort included 26 (38.8%) left calcaneal fractures, 27 (40.30%) right calcaneal fractures, and 14 (20.9%) cases with bilateral fractures. Comminuted fractures accounted for 92.5% of all fractures. Sagittal 3D mapping demonstrated that fracture lines were concentrated in the critical angle of Gissane and extended posteriorly to the rear of the tuberosity of the lateral wall and the anterior of the medial process of the calcaneus tuberosity but with more significant variation in the medial wall. The mean angle of fracture lines concerning the long axis of the calcaneal (LAC) was 29.1°and 19.2° in the lateral wall and medial wall, respectively. Axial 3D mapping demonstrated that fracture lines were primarily concentrated in the area anterior to the posterior joint facet and extended along the posterior joint facet and calcaneus sulcus to the posteriorly of the tuberosity. The mean angle of fracture lines concerning the LAC was 11° in the axial wall. 3D mapping demonstrated that the comminution zones are closely related to the internal structure and fracture mechanism. Conclusion: The data presented have elucidated there are consistent characteristic fracture patterns and comminution zones for ICFs. This study provides visual guidelines to understand fracture morphology, which may assist with fracture classification, preoperative planning, fixation concepts development, and internal structure analysis.
Synthesis, and Biological Evaluations of Novel Pyrazinoic Acid Derivatives as Anticancer Agents
Authors: Akhlaghi Shiva , mostoufi Azar , kalantar Hadi , Fereidoonnezhad Masood 
Published: October 2021

Abstract
Pyrazinoic acid or pyrazine-2-carboxylic acid (PA), due to its nitrogenous heteroaromatic ring, can be explored as an anticancer agent. Here, a series of twenty novels PA derivatives have been synthesized and characterized using IR, NMR, and mass spectrums. Their cytotoxic activity was evaluated against three different cancer cell lines, including lung (A549), breast (MCF-7), and colon (HT-29). P16, the most potent compound, showed moderate cytotoxicity with IC50 of 6.11 μM, 10.64 μM, and 14.92 μM, against the A549, MCF-7, and HT-29 cell lines, respectively. Furthermore, the effect of this compound against MRC5 as a non-tumoral lung cell line, exhibited a selectivity index of 9.02. The apoptotic induction activity of P16 was also performed on the A549 cell line. The results showed that as the concentration of the compound increases (from 3 to 6 μM), the percentage of induction of apoptosis increases from 8.54% to 72.4%. Electrophoretic gel mobility shift assays showed that P16 was also able to ROS induce DNA cleavage in the presents of H2O2 (1.0 mM) in dose-dependent manner. Molecular docking was also applied to anticipate the binding locations and the binding of the synthesized compound with Bcl-2 apoptosis regulator and DNA as their proposed targets.
Encoding and retrieval of associative recognition memory engage different sub-networks within a hippocampal-thalamo-cortical memory circuit
Authors: Barker Gareth , Tran Stephanie , Gilroy Kerry , Bashir Zafar , Warburton Elizabeth 
Published: October 2021

Abstract
Recognition of previously encountered stimuli and their associated spatial and temporal information depends on neural activity within a brain-wide network in which the CA1 region of the hippocampus, nucleus reuniens of the thalamus (NRe) and medial prefrontal cortex (mPFC) are key nodes. However, the pathways crucial for coordinating activity during memory encoding and/or retrieval phases have been little explored. Here we opto- or chemo associative recognition memory. We discovered that encoding, but not retrieval depended on the CA1 to mPFC and NRe to mPFC projections. In contrast, retrieval depended on the mPFC to NRe projection. Interestingly the NRe to CA1 pathway was required for both memory phases. Our findings therefore reveal that encoding and retrieval engage dissociable sub-networks within a hippocampal-thalamo-cortical recognition memory circuit in order to enable binding of recent and related information, whilst ensuring a separation of processing.
Assessing The Long- and Short-Run Asymmetrical Effects of Climate Change On Rice Production: Empirical Evidence From India
Authors: Baig Imran Ali , Chandio Abbas Ali , Ozturk Ilhan , Kumar Pushp , Khan Zeeshan Anis , Salam Md. Abdus 
Published: October 2021

Abstract
For a couple of decades, environmental change has arisen as a ubiquitous problem and gained environmentalist's attention across the globe due to its long-term harmful effect on agricultural production, food supply, water supply and livelihoods of rural poor. The primary objective of this study is to explore the asymmetrical dynamic relationship between climate change and production of rice and controlled variables covering 1991–2018 by employing the nonlinear autoregressive distributed lag (NARDL) model and Granger causality approach.in India. The NARDL findings demonstrate a significant negative relationship between mean temperature and production of rice in the long run while positively influencing rice production in the short run. Moreover, positive shocks in rainfall and carbon emission have a negative and significant effect on India's rice production in the long and short run. In comparison, negative shock in rainfall has a significant positive impact on rice production in the long and short run. Wald test confirms the asymmetrical relationship between climate change and rice production. The Granger causality test shows feedback effect among mean temperature, decreasing rainfall, increasing carbon emission, and rice production. While no causal relationship between increasing temperature and decreasing carbon emission. Based on our empirical investigations, some critical policy implications emerged. To sustain rice production, improve irrigation infrastructure through increasing public investment and develop climate-resilient seeds varieties to cope with climate change. Along with, at the district level government should provide proper training to farmers regarding the usage of pesticides, proper amount of fertiliser and irrigation systems.
Effect of Total Anthocyanin-base Standardized Cornus Mas L. Fruit Extract on Hepatic Steatosis and Visceral Adiposity Index in Patients With Non-alcoholic Fatty Liver Disease: a Double-blind Randomized Controlled Trial
Authors: Sangouni Abbas Ali , Hosseinzadeh Mahdieh , Sangsefidi Zohreh Sadat , Yarhosseini Faezeh , Akhondi-Meybodi Mohsen , Ranjbar Alimohammad , Madadizadeh Farzan , Mozaffari-Khosravi Hassan 
Published: October 2021

Abstract
Background:Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide. Experimental evidence has proposed the beneficial effects of Cornus mas L. (cornelian cherry) extract, as a rich source of anthocyanins, on steatosis and central obesity. However, very few clinical trials were conducted in this regard. We investigated the effect of total anthocyanin-base standardized cornelian cherry fruit extract on hepatic steatosis index (HSI) and NAFLD liver fat score (NAFLD-LFS), and visceral adiposity index (VAI) in patients with NAFLD.Methods:The present study was conducted as a double-blind, randomized controlled clinical trial among 50 subjects suffering from NAFLD. Subjects were randomly assigned to receive cornelian cherry extract (20 cc/d, which provides 320 mg/d total anthocyanin) or placebo for 12 weeks.Results:There was no difference between the two groups in HSI, NAFLD-LFS and VAI at the baseline. After 12-week intervention, cornelian cherry extract compared to the placebo demonstrated no significant effect on HSI (change: -0.9 ± 3.5 vs. -1.2 ± 3.2; P = 0.72), NAFLD-LFS (change: 0.2 ± 1.3 vs. 0.1 ± 0.7; P = 0.62), and VAI (change: 0.05 ± 0.7 vs. 0.07 ± 1.0; P = 0.94).Conclusion:Cornelian cherry extract (20 cc/d) for 12 weeks has no effect on steatosis and visceral obesity. To better conclude, further trials with longer intervention durations are required. Trial registration: The study protocol was registered on 30 September 2018 at Iranian Registry of Clinical Trials under code IRCT20180419039359N1 (https://www.irct.ir/trial/30707).
Plate motion drives variability in ocean oxygenation through the Phanerozoic
Authors: Pohl Alexandre , Ridgwell Andy , Stockey Richard , Thomazo Christophe , Keane Andrew , Vennin Emmanuelle , Scotese Christopher 
Published: October 2021

Abstract
The early evolutionary and much of the extinction history of marine animals, is thought to be driven by changes in dissolved oxygen concentrations ([O2]) in the ocean1–3. In turn, [O2] is widely assumed to be dominated by the geological history of atmospheric oxygen (pO2)4,5. Here, in contrast, we show via a series of Earth system model experiments how continental rearrangement during the Phanerozoic drives profound variations in ocean oxygenation and induces a fundamental decoupling in time between upper-ocean and benthic [O2]. We further identify the presence of (paleogeographical) state-dependent modes of circulation instability (oscillations), which allows for pO2 to already be close to modern by the beginning of the Phanerozoic despite the deep ocean remaining anoxic. The occurrence of extreme volatility in ocean oxygenation also provides a causal mechanism explaining elevated rates of metazoan radiation and extinction during the early Paleozoic6. The absence in our modelling of any simple correlation between global climate and ocean ventilation together with the occurrence of profound variations in ocean oxygenation independent of atmospheric pO2, presents both a challenge to the interpretation of marine redox proxies, but also points to a hitherto unrecognized role for continental configuration in the evolution of the biosphere.
Oocytes with smooth endoplasmic reticulum aggregates do not impact blastocyst euploidy rate
Authors: Xu Jian , Yang Li , Chen Zhi-Heng , Yin Min-Na , Chen Juan , Sun Ling 
Published: October 2021

Abstract
Objective: To investigate whether the euploidy rate of blastocysts derived from smooth endoplasmic reticulum (SERa) positive cycles and oocytes are impacted.Design: Retrospective cohort study.Setting: A tertiary hospital-based reproductive medicine center.Patient(s): A total of 601 preimplantation genetic testing (PGT) cycles with obtained oocytes in our center between April 2017 and May 2021 were included in the study. Intervention(s): Women＞35 years and PGT cycles with chromosomal structural rearrangements (PGT-SR) were excluded. Embryological and blastocyst ploidy outcomes were compared between SERa+ oocyte, sibling SERa- oocytes and oocytes in SERa- cycles.Main Outcome Measure(s): Embryological outcomes and blastocyst euploidy rate.Results: No significant difference was observed in the normal fertilization rate (82.1 % vs. 77.8 % vs. 83.1 %, respectively, P=0.061), blastocyst formation rate (71.0 % vs. 72.5 % vs. 68.4 %, respectively, P=0.393), good quality blastocyst formation rate (46.4 % vs. 48.3 % vs. 42.6 %, respectively, P=0.198) between the SERa+ oocyte group, sibling SERa- oocyte group and SERa- oocyte group. No significant difference was observed in the euploidy rate (50.0 % vs. 62.5 % vs. 63.3 %, respectively, P=0.324), mosaic rate (12.5 % vs. 9.7 % vs. 13.4 %, respectively, P=0.506) and aneuploidy rate (37.5 % vs. 27.8% vs. 23.2 %, respectively, P=0.137) between the three groups.Conclusion: Our results suggest that the euploidy rate of blastocysts derived from SERa+ cycles and oocytes are not impacted.
Metabolic Profiling and Inhibitory Properties of Different Parts of Salsola Vermiculata Towards Acetylcholinesterase and α-glucosidase
Authors: Mollaei Saeed , Farnia Poopak , Ghanavi Jalaledin 
Published: October 2021

Abstract
Background: Herbal and natural medicines play significant roles in treatment of diseases and development of novel drugs. Salsola vermiculata is an annual plant which is broadly distributed in southwest of Asia, and is used for treatment of stomach disorders. Results: This present study aimed at identifying and comparing the metabolic profiles of different parts of Salsola vermiculata and to evaluate the inhibitory potential of their extracts and fractions towards acetylcholinesterase and α-glucosidase. LC-ESI-MS, GC, and GC-MS analytical methods were employed for metabolite profiling of the extracts, and their fractions. The α-glucosidase and acetylcholinesterase inhibitory activities of the samples were determined by microplate colorimetric methods. Based on results, 44 metabolites were identified in different parts of S. vermiculata. In roots, vanillic acid, rutin, salsoline, salsoline A, palmitic acid, oleic acid, linoleic acid, cumin aldehyde, and carvone; in seeds, vanillic acid, salsoline A, palmitic acid, oleic acid, linoleic acid, carvone, and β-caryophyllene; in leaves, gallic acid, vanillic acid, caffeic acid, rosmaric acid, rutin, quercetin, limonene, and carvone, and in flowers, gallic acid, vanillic acid, cinnamic acid, rosmaric acid, rutin, kaempferol, limonene, linalool, and carvone were recorded as the main components. According to the inhibitory activities results, the ethyl acetate fractions of leaves and the aqueous-acid fraction of roots displayed highest inhibitory activity towards acetylcholinesterase (IC50: 17.24 µg/mL), and α-glucosidase (IC50: 62.37 µg/mL), respectively. Conclusion: Finally, the leaves and roots of S. vermiculata are rich of phenolic and alkaloids compounds and the findings of this study depict them as a promising acetylcholinesterase and α-glucosidase inhibitors, and therefore, can be utilized for the development of new drugs.