bluuebunny/crossref_metadata_2025_split huggingface dataset:
Air pollutants are negatively associated with vitamin D-synthesizing UVB radiation intensity on the ground
Authors: Rahman Abdur , Elmi Abdirashid 
Published: November 2021

AbstractAtmospheric levels of pollutants may reduce the UVB intensity at the earth’s surface, with a subsequent reduction in cutaneous vitamin D synthesis. We investigated the association of various pollutants with UVB intensity on the ground. Four-year data obtained from four weather stations from across Kuwait were analyzed by median regression. Pollutants that were negatively associated with UVB were [β (95% CI)]: benzene [− 2.61 (− 4.13, − 1.09)], ethyl-benzene [− 2.20 (− 3.15, − 1.25)], ozone [− 0.23 (− 0.28, − 0.17)], nitric oxide [− 0.11 (− 0.15, − 0.06)], sulfur dioxide [− 0.10 (− 0.17, − 0.04)] and particulate matter PM10 [− 0.002 (− 0.003, − 0.002)]. Pollutants that were negatively associated with the UVB/UVA ratio were [β (95% CI)]: benzene [− 15.57 (− 24.94, − 6.20)], nitric oxide [− 0.53 (− 0.81, − 0.25)], ozone [− 0.38 (− 0.70, − 0.06)], and total hydrocarbon [− 0.02 (− 0.04, − 0.01)]. Furthermore, benzene and nitric oxide levels were higher in the morning and evening hours, which are the times of most solar exposure in this region due to high temperature during midday. In addition to other known factors, attenuation of UVB by these pollutants may contribute to lower vitamin D levels in populations. In addition to direct public health hazard, these pollutants may contribute to the very high prevalence of VDD in this region.
Differential urine proteome analysis of a ventilator-induced lung injury rat model by label-free quantitative and parallel reaction monitoring proteomics
Authors: Qin Weiwei , Zhang Xiao , Chen Lingnan , Li Qiujie , Zhang Benwang , Sun Lixin , Han Wei 
Published: November 2021

AbstractUrine is a promising resource for biomarker research. Therefore, the purpose of this study was to investigate potential urinary biomarkers to monitor the disease activity of ventilator-induced lung injury (VILI). In the discovery phase, a label-free data-dependent acquisition (DDA) quantitative proteomics method was used to profile the urinary proteomes of VILI rats. For further validation, the differential proteins were verified by parallel reaction monitoring (PRM)-targeted quantitative proteomics. In total, 727 high-confidence proteins were identified with at least 1 unique peptide (FDR ≤ 1%). Compared to the control group, 110 proteins (65 upregulated, 45 downregulated) were significantly changed in the VILI group (1.5-fold change, P < 0.05). The canonical pathways and protein–protein interaction analyses revealed that the differentially expressed proteins were enriched in multiple functions, including oxidative stress and inflammatory responses. Finally, thirteen proteins were identified as candidate biomarkers for VILI by PRM validation. Among these PRM-validated proteins, AMPN, MEP1B, LYSC1, DPP4 and CYC were previously reported as lung-associated disease biomarkers. SLC31, MEP1A, S15A2, NHRF1, XPP2, GGT1, HEXA, and ATPB were newly discovered in this study. Our results suggest that the urinary proteome might reflect the pathophysiological changes associated with VILI. These differential proteins are potential urinary biomarkers for the activity of VILI.
Simultaneous expression of MMB-FOXM1 complex components enables efficient bypass of senescence
Authors: Kumari Ruchi , Hummerich Holger , Shen Xu , Fischer Martin , Litovchick Larisa , Mittnacht Sibylle , DeCaprio James A. , Jat Parmjit S. 
Published: November 2021

AbstractCellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21WAF1/CIP1 and pRB/p16INK4A tumour suppressor pathways, the downstream targets responsible for the stability of the growth arrest are not known. We have employed a stable senescence bypass assay in conditionally immortalised human breast fibroblasts (CL3EcoR) to investigate the role of the DREAM complex and its associated components in senescence. DREAM is a multi-subunit complex comprised of the MuvB core, containing LIN9, LIN37, LIN52, LIN54, and RBBP4, that when bound to p130, an RB1 like protein, and E2F4 inhibits cell cycle-dependent gene expression thereby arresting cell division. Phosphorylation of LIN52 at Serine 28 is required for DREAM assembly. Re-entry into the cell cycle upon phosphorylation of p130 leads to disruption of the DREAM complex and the MuvB core, associating initially to B-MYB and later to FOXM1 to form MMB and MMB-FOXM1 complexes respectively. Here we report that simultaneous expression of MMB-FOXM1 complex components efficiently bypasses senescence with LIN52, B-MYB, and FOXM1 as the crucial components. Moreover, bypass of senescence requires non-phosphorylated LIN52 that disrupts the DREAM complex, thereby indicating a central role for assembly of the DREAM complex in senescence.
Measurement of chest wall motion using a motion capture system with the one-pitch phase analysis method
Authors: Tamiya Hiroyuki , Mitani Akihisa , Isago Hideaki , Ishimori Taro , Saito Minako , Jo Taisuke , Tanaka Goh , Yanagimoto Shintaro , Nagase Takahide 
Published: November 2021

AbstractSpirometry is a standard method for assessing lung function. However, its use is challenging in some patients, and it has limitations such as risk of infection and inability to assess regional chest wall motion. A three-dimensional motion capture system using the one-pitch phase analysis (MCO) method can facilitate high precision measurement of moving objects in real-time in a non-contacting manner. In this study, the MCO method was applied to examine thoraco-abdominal (TA) wall motion for assessing pulmonary function. We recruited 48 male participants, and all underwent spirometry and chest wall motion measurement with the MCO method. A significant positive correlation was observed between the vital capacity (Spearman’s ρ = 0.68, p < 0.0001), forced vital capacity (Spearman’s ρ = 0.62, p < 0.0001), and tidal volume (Spearman’s ρ = 0.61, p < 0.0001) of spirometry and the counterpart parameters of MCO method. Moreover, the MCO method could detect regional rib cage and abdomen compartment contributions and could assess TA asynchrony, indicating almost complete synchronous movement (phase angle for each compartment: − 5.05° to 3.86°). These findings suggest that this technique could examine chest wall motion, and may be effective in analyzing chest wall volume changes and pulmonary function.
A complete sequence of mitochondrial genome of Neolamarckia cadamba and its use for systematic analysis
Authors: Wang Xi , Li Ling-Ling , Xiao Yu , Chen Xiao-Yang , Chen Jie-Hu , Hu Xin-Sheng 
Published: November 2021

AbstractNeolamarckia cadamba is an important tropical and subtropical tree for timber industry in southern China and is also a medicinal plant because of the secondary product cadambine. N. cadamba belongs to Rubiaceae family and its taxonomic relationships with other species are not fully evaluated based on genome sequences. Here, we report the complete sequences of mitochondrial genome of N. cadamba, which is 414,980 bp in length and successfully assembled in two genome circles (109,836 bp and 305,144 bp). The mtDNA harbors 83 genes in total, including 40 protein-coding genes (PCGs), 31 transfer RNA genes, 6 ribosomal RNA genes, and 6 other genes. The base composition of the whole genome is estimated as 27.26% for base A, 22.63% for C, 22.53% for G, and 27.56% for T, with the A + T content of 54.82% (54.45% in the small circle and 54.79% in the large circle). Repetitive sequences account for ~ 0.14% of the whole genome. A maximum likelihood (ML) tree based on DNA sequences of 24 PCGs supports that N. cadamba belongs to order Gentianales. A ML tree based on rps3 gene of 60 species in family Rubiaceae shows that N. cadamba is more related to Cephalanthus accidentalis and Hymenodictyon parvifolium and belongs to the Cinchonoideae subfamily. The result indicates that N. cadamba is genetically distant from the species and genera of Rubiaceae in systematic position. As the first sequence of mitochondrial genome of N. cadamba, it will provide a useful resource to investigate genetic variation and develop molecular markers for genetic breeding in the future.
Evolution of floral characters and biogeography of Heloniadeae (Melanthiaceae): an example of breeding system shifts with inflorescence change
Authors: Chao Chien-Ti , Kuo Chu-Chia , Chang Jui-Tse , Chai Min-Wei , Liao Pei-Chun 
Published: November 2021

AbstractHeloniadeae (Melanthiaceae) presents an East Asia–North America disjunct distribution. Different molecular and morphological data nevertheless support the tribe as a monophyletic group. However, their phylogenetic relationships and biogeographic history, together with the character evolution, are not clear. Therefore, we constructed a Bayesian phylogenetic tree for Heloniadeae using cpDNA and inferred the historical biogeography and floral character evolution. The results revealed that Heloniadeae was distributed in high-latitudes of East Asia and North America, originating since 22.2 mya. The East Asia clade migrated into southwest China, and subsequently colonized the Korean Peninsula, Taiwan, the Ryukyus, and spread northward to Japan and southern Sakhalin. The evolution of the inflorescence and number of flowers were phylogenetically conserved, associated with the historical biogeography of Heloniadeae. The inflorescences transferred from raceme to sub-umbel, and the number of flowers decreased during the dispersal process, which may be accompanied by changes in the breeding system. Besides, the anthesis period was more affected by the habitat environment than phylogenetic constraints. The flowering temperature of Heloniadeae was below 20 °C in most species, except H. kawanoi. Such a low temperature might not be conductive to pollinator activities, but it could be compensated by sustaining seed production with long-lasting flowers.
Hemin as a novel candidate for treating COVID-19 via heme oxygenase-1 induction
Authors: Kim Dong-Hwi , Ahn Hee-Seop , Go Hyeon-Jeong , Kim Da-Yoon , Kim Jae-Hyeong , Lee Joong-Bok , Park Seung-Yong , Song Chang-Seon , Lee Sang-Won , Ha Sang-Do , Choi Changsun , Choi In-Soo 
Published: November 2021

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 (COVID-19). More than 143 million cases of COVID-19 have been reported to date, with the global death rate at 2.13%. Currently, there are no licensed therapeutics for controlling SARS-CoV-2 infection. The antiviral effects of heme oxygenase-1 (HO-1), a cytoprotective enzyme that inhibits the inflammatory response and reduces oxidative stress, have been investigated in several viral infections. To confirm whether HO-1 suppresses SARS-CoV-2 infection, we assessed the antiviral activity of hemin, an effective and safe HO-1 inducer, in SARS-CoV-2 infection. We found that treatment with hemin efficiently suppressed SARS-CoV-2 replication (selectivity index: 249.7012). Besides, the transient expression of HO-1 using an expression vector also suppressed the growth of the virus in cells. Free iron and biliverdin, which are metabolic byproducts of heme catalysis by HO-1, also suppressed the viral infection. Additionally, hemin indirectly increased the expression of interferon-stimulated proteins known to restrict SARS-CoV-2 replication. Overall, the findings suggested that HO-1, induced by hemin, effectively suppressed SARS-CoV-2 in vitro. Therefore, HO-1 could be potential therapeutic candidate for COVID-19.
Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
Authors: Rodrigues Aline Q. , Picolo Victor L. , Goulart Jair T. , Silva Isabella M. G. , Ribeiro Rayane B. , Aguiar Beatriz A. , Ferreira Yasmin B. , Oliveira Daniela M. , Lucci Carolina M. , de Bem Andreza F. , Paulini Fernanda 
Published: November 2021

AbstractCryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.
A pre-formulation study of tetracaine loaded in optimized nanostructured lipid carriers
Authors: Castro Simone R. , Ribeiro Lígia N. M. , Breitkreitz Márcia C. , Guilherme Viviane A. , Rodrigues da Silva Gustavo H. , Mitsutake Hery , Alcântara Ana C. S. , Yokaichiya Fabiano , Franco Margareth K. K. D. , Clemens Daniel , Kent Ben , Lancellotti Marcelo , de Araújo Daniele R. , de Paula Eneida 
Published: November 2021

AbstractTetracaine (TTC) is a local anesthetic broadly used for topical and spinal blockade, despite its systemic toxicity. Encapsulation in nanostructured lipid carriers (NLC) may prolong TTC delivery at the site of injection, reducing such toxicity. This work reports the development of NLC loading 4% TTC. Structural properties and encapsulation efficiency (%EE > 63%) guided the selection of three pre-formulations of different lipid composition, through a 23 factorial design of experiments (DOE). DLS and TEM analyses revealed average sizes (193–220 nm), polydispersity (< 0.2), zeta potential |− 21.8 to − 30.1 mV| and spherical shape of the nanoparticles, while FTIR-ATR, NTA, DSC, XRD and SANS provided details on their structure and physicochemical stability over time. Interestingly, one optimized pre-formulation (CP-TRANS/TTC) showed phase-separation after 4 months, as predicted by Raman imaging that detected lack of miscibility between its solid (cetyl palmitate) and liquid (Transcutol) lipids. SANS analyses identified lamellar arrangements inside such nanoparticles, the thickness of the lamellae been decreased by TTC. As a result of this combined approach (DOE and biophysical techniques) two optimized pre-formulations were rationally selected, both with great potential as drug delivery systems, extending the release of the anesthetic (> 48 h) and reducing TTC cytotoxicity against Balb/c 3T3 cells.
Close binary evolution based on Gaia DR2
Authors: Peng Weiguo , Song Hanfeng , Meynet Georges , Maeder Andre , Barblan Fabio , Zhang Ruiyu , Ekströmt Sylvia , Georgy Cyril , Long Gang , Zhao Liuyan , Qin Ying 
Published: November 2021

Context. The observed late-type WC Wolf-Rayet stars (WC7-9) with low luminosity below log L/L⊙ < 5.4 in the HR diagram cannot be reproduced satisfactorily by the evolutionary track of single stars. The mass transfer due to Roche lobe overflow drastically modifies the internal structure and surface compositions of two components. Therefore, binaries provide a very promising evolutionary channel to produce these WC stars.
Aims. The Gaia satellite provides accurate distances to WC stars and confirms the luminosities of WC stars. Based on a small grid containing single stars and binaries, we aim to investigate the extent to which the evolution of a single or a close binary can reproduce the properties of these stars.
Methods. We considered single-star models with masses between 20 and 40 M⊙. We calculated the evolution for three binaries with a 30 M⊙ primary star with a 27 M⊙ companion star with initial orbital periods of 6.0, 20.0, 500.0, and 1000.0 days.
Results. The rotating single star can evolve into a late-type WC star but with high luminosity (i.e., log L/L⊙ > 5.4). Enhanced wind mass loss rates during RSG and WR stages, as proposed in the literature, can cause the star to approach the observational range of low-luminosity WC stars and favor the formation of low-luminosity WO stars. In a wide binary system with initial Porb = 1000 days, the primary star can evolve into a late-type WC star and be compatible with the observed properties of the low-luminosity WC stars. The result is almost insensitive to the adopted accretion efficiency 1 − β.
Conclusions. Compared with single stars, the low brightness is due to a smaller temperature gradient inside the star after the Case C Roche lobe overflow, while the low effective temperature is due to envelope expansion. There are four physical reasons for the formation of the expanding envelope. Firstly, less helium envelope can be transferred to the companion star in this system. Heavy helium envelopes can be heated by the helium burning shell and this creates the necessary conditions for the envelope expansion. Secondly, the expansion of the helium envelope can also be boosted by the sharp shrinkage of the larger carbon-oxygen core through the mirror effect. Thirdly, a more massive WC star can attain a higher Eddington factor because of its higher L/M ratio. The increase in L/M with mass is the primary cause for the extended envelopes in WC stars. Finally, the iron opacity bump at T ∼ 105.25 K may also trigger envelope inflation because it can lead to a larger Eddington factor.
Cationic Iridium/Chiral Bidentate Phosphoramidite Catalyzed Asymmetric Hydroarylation
Authors: Yamamoto Yasunori , Shirai Tomohiko 
Published: November 2021

AbstractIn this personal account, we summarize our investigations on the asymmetric direct addition of C(sp2)–H bonds to unsaturated bonds, such as C=O and C=C, using cationic iridium/chiral O-linked bidentate phosphoramidite (Me-BIPAM) and S-linked bidentate phosphoramidite (S-Me-BIPAM) catalyst systems.1	Introduction2	Highly Enantioselective Intramolecular Hydroarylation of α-Keto Amides3	Highly Enantioselective Intermolecular Hydroarylation of Bicyclo­alkenes4	Conclusion
Vacuum-Assisted Excision, Scarless Solution for Fibroadenoma Breast—A Single-Center Experience
Authors: Rupa R. , Kushvaha Suchana 
Published: November 2021

Abstract
Background Fibroadenoma is one of the most common benign breast lesions that is frequently surgically excised in breast practice.
Aim The aim of this study was to determine the efficacy and safety of ultrasound-guided (USG) vacuum-assisted excision biopsy (VAEB) of fibroadenoma.
Settings and Design A retrospective observational study.
Methods and Materials A total of 113 patients with 163 fibroadenomas who underwent USG-guided VAEB under local anesthesia with 7G and 10G probes using an Encor Enspire equipment were included in the study. The patients were followed up after 1, 6, and 12 months. The fibroadenomas up to 4 cm were excised as per the U.S. Food and Drug Administration-approved guidelines of the American Society of Surgeons for percutaneous removal of benign breast lesions.
Results The percentage of complete excision rate was 98.8% and the most frequent complication encountered was hematoma including immediate and delayed accounting for 3.06%. There were no recurrent lesions in our study.
Conclusion USG-guided VAEB of benign breast lumps can be a safe and effective alternative to surgical excision and had better patient satisfaction in terms of efficacy and cosmesis.
Tumors of the Acromion Process—A Pictorial Review
Authors: Boo S.L. , Saad A. , Khan Z. , Davies A. M. , James S. L. , Botchu R. 
Published: November 2021

Abstract
Introduction Acromion is essential for stabilizing the shoulder complex. Tumors of the acromion are rare. We report the largest series of acromion tumor and tumor-like lesion.
Materials and Methods A retrospective review of the oncology and radiology database within our tertiary center for orthopaedic oncology was performed to identify all tumors of the acromion over the past 30 years and imaging was reviewed.
Results We identified a total of 31 lesions arising in the acromion and chondrosarcoma was the commonest.
Conclusion One needs to be aware of tumor and tumor-like lesions of acromion.
Pyomyositis of the Piriformis Muscle—A Case of Piriformis Syndrome
Authors: Shanmuga Jayanthan S , Rajkumar S. Senthil , Kumar V. Senthil , Shalini M. 
Published: November 2021

AbstractPiriformis syndrome is a rare cause of sciatica, which results in low backache due to sciatic nerve compression. This syndrome is associated with abnormalities in the piriformis muscle, which cause sciatic nerve entrapment, like anatomical variations, muscle hypertrophy, and inflammation. It can also result from the abnormal course of sciatic nerve itself through normal piriformis muscle. Piriformis syndrome due to pyomyositis of the piriformis muscle is extremely rare and only 23 cases are reported in literature. Herein, we report one such rare case of a patient, with pyomyositis of piriformis muscle, who presented with piriformis syndrome.
Dynamics of vertical-cavity surface-emitting lasers under AM and FM current modulations
Authors: Temgoua Loannis Pavel Gauss , Talla Mbé Jimmi Hervé , Essimbi Zobo Bernard , Woafo Paul 
Published: November 2021

Abstract
We numerically investigate the dynamics of Vertical-Cavity Surface-Emitting Lasers (VCSELs) described by the current-dependent gain model and subjected to current in the form of amplitude modulation (AM) and frequency modulation (FM). Since VCSELs have two polarization modes (PMs), the competition between them offers more complex dynamical behaviors. It is found that when operating close to the threshold current, the laser converts the AM and FM currents into a series of pulse packages (PPs) that are globally irregular and modulated either in amplitude or frequency. In the particular case of FM current, both modulations occur in a unique signal. Besides, chaotic light is recovered even at low frequencies (few MHz) where it is not usually expected when sinusoidal current modulation is used. But, if the AM and the FM currents evolve over the threshold value, the laser does not alter the form of the current. Therefore, those currents are linearly converted into light signals.
Theoretical description of pygmy and giant dipole resonances in 237Np
Authors: Yakut H , Tabar E , Kemah E , Hoşgör G 
Published: November 2021

Abstract
This study presents the descriptive characteristics of the electric dipole (E1) excitations for the first time in the 237Np nucleus, such as the reduced transition probability, the photo-absorption cross-section, the energy-weighted sum rule, and so on, as calculated by the Translational and Galilean Invariant Quasiparticle Phonon Nuclear Model (TGI-QPNM). To accurately determine the dipole properties of the collective pygmy and giant resonances, it was necessary to eliminate the spurious states mixed into the dipole spectrum because the mean-field Hamiltonian breaks the translational invariance symmetry. We therefore also investigated the effect of spurious states on the PDR and GDR spectra using the TGI-QPNM method. In addition, we consulted the available knowledge for the energy-weighted sum rule to test the TGI-QPNM model, and this resulted in 97% accuracy for E1 dipole excitations. Furthermore, the theoretical results were compared with experimental data for the photo-absorption cross-sections in the 5–20 MeV energy range for the 237Np nucleus, with a satisfactory agreement being found.