bluuebunny/crossref_metadata_2025_split huggingface dataset:
AEBP1 is Upregulated in Diabetic Nephropathy Biopsies
Authors: Freije Benjamin , Ferreira Ricardo Melo , Cheng Ying-Hua , Parikh Samir , Eadon Michael 
Published: December 2021

Background: Worldwide, one in eleven adults have diabetes mellitus and 30% to 40% will develop diabetic kidney disease (DKD). A mechanistic understanding of DKD is crucial to develop treatment strategies. To unravel DKD’s pathogenesis, single cell (scRNA) sequencing has proven a powerful tool, but is limited by a lack of localization. Spatial transcriptomics allows the mapping of scRNA sequencing data back to histology.
Methods: Frozen human nephrectomy and biopsy samples were processed according to Visium spatial gene expression protocols, stained with H&E, and imaged. Samples were permeabilized for RNA capture, reverse transcribed and sequenced on an Illumina NovaSeq 6000. Mapping and counting were completed in Space Ranger and data was processed in Seurat. Samples were laser microdissected, protein was isolated, and protein was quantified by HPLC-MS.
Results: Clusters from scRNAseq were mapped upon reference and DKD spatial transcriptomic images (N=4 reference, 2 DKD). Differentially expressed genes were identified in diabetic kidneys, including the upregulation of Adipocyte Enhancer Binding Protein (AEBP1). Pathway analysis revealed enrichment of extracellular matrix organization and immune process pathways. To increase the confidence of these findings, glomeruli and the tubulointerstitium were laser microdissected (N=7 diseased, 4 reference) for proteomic analysis. AEBP1 was upregulated in the tubular interstitium of diseased kidneys and selectively upregulated in the glomeruli of Diabetic Nephropathy samples (N=2). AEBP1 localized to the interstitium by spatial transcriptomics and was expressed in highly fibrotic regions. Glomerular expression was not observed due to glomerulosclerosis.
Conclusion: AEBP1 upregulation is a marker of interstitial fibrosis, with specific expression in the glomeruli of diabetic nephropathy specimens with glomerulosclerosis.
Impact: This is the first study utilizing spatial transcriptomics to define and localize markers of human kidney disease. Confirmatory studies are required in larger sample sizes. AEBP1 is a previously unidentified marker of DKD previously associated with fibrosis in other organ-specific diseases.
We’re in This Together: Community Coalition Approaches to Treating Depression
Authors: Jeong Naari , Savaiano Dennis 
Published: December 2021

Background and Objective: Depression is the leading cause of disability worldwide, yet approximately one-third of adults diagnosed with major depressive disorder do not receive treatment. Health coalitions are one strategy towards addressing this issue through collaborative, multi-sector interventions implemented within community organizations. In this narrative review, we examine what evidence exists that coalitions may improve outcomes of depression.

Methods: A search for peer-reviewed literature was conducted using PubMed, CINHAL, MEDLINE, and PsychINFO databases. The search was limited to studies published in the English language. No limitations were placed on location or date of publication. Initial search produced a total of 236 articles. Of these, 34 met inclusion criteria. Papers that did not address depression interventions in the context of community health coalitions were excluded.

Results: Community Partners in Care, a double-blinded randomized control trial, is the primary model in literature that describes outcomes of coalitions in addressing depression. 6-month outcomes revealed that participants treated through a coalition model (n=504) as opposed to those in a non-coalition approach (n=512) had significantly improved self-reported mental health related quality of life and a reduced number of behavioral health hospitalizations. 3-year and 4-year outcomes revealed participants in the coalition model had increased odds of clinical depression remission. While many other studies documented coalition-based interventions, there was little to no report of treatment effectiveness.

Project Impact: Current literature suggests that coalitions may be an effective method of addressing depression, particularly in under-resourced areas where community members are more likely to access care through social services. In addition, churches and schools were identified as key partners for coalitions, as trusted informal networks for mental health support. While results support coalition utility for mental health intervention, more research is needed to determine what, if any, unique attributes of coalitions are necessary to insure effective mental health interventions.
Rates of Ischemia Amongst Asymptomatic Patients with High Coronary Artery Calcium Scores
Authors: Jones Aaron , Thomas Dustin 
Published: December 2021

CACS may be used to recommend lifestyle changes or other treatment recommendations to reduce the risk of heart disease. The incidence of asymptomatic ischemia in patients with elevated CACS is poorly defined. Furthermore, the CACS cut-off above which it is clinically important and cost effective to look for and diagnose ischemia is also not well defined. The purpose of this study is to better define the incidence of asymptomatic ischemia in Parkview Heart Institute (PHI) patients with elevated CACS, including an examination of demographic data that may also influence the rate of ischemia. 118 Patients who were screened for CAD, received CACS ≥ 400, and subsequently had stress testing or invasive coronary angiogram within 6 months were admitted into the study. Through statistical analysis it was determined there was no statistical difference in rates of ischemia between patients with CACS of 400-999 and those >1000. However, due to a small sample size this study will be continued to strengthen its results and investigate if demographics play a role in rates of ischemia.
Treatment of FRI after intramedullary nailing of tibia fractures: assessment of nail retention and nail removal
Authors: Jones Jenna , Mullis Brian , Weaver Bree , Natoli Roman 
Published: December 2021

Objectives: Fracture related infection (FRI) is a severe, potentially limb-threatening complication after fracture fixation. Dilemma exists with regard to removing or retaining implants while treating the infection. The purpose of this study was to compare primary bone union and infection clearance in patients who had an infection following intramedullary nailing of the tibia treated either by retaining the implant or by removing the implant.

Methods: Patients from two level-I trauma centers were identified through billing registries and retrospectively reviewed between January 2013 and December 2020. We identified 44 patients who had a diagnosis of FRI within 90 days of their initial fixation and returned to the OR for operative treatment of the infection. The incidences of both primary union and infection clearance were calculated for both groups and multiple parameters that may be associated with success or failure were assessed.

Results: Four patients did not have complete records and were excluded. Of the remaining patients, 20 (50%) achieved infection clearance. Twenty-three (59%) patients achieved primary union whereas 16 (41%) had a primary outcome of either delayed union, nonunion, or amputation (one additional patient excluded as healing status unknown). Further analysis showed no significant difference (X2 (39) = 1.13, p < .29) in infection clearance between patients treated with nail retention (64%) versus nail removal (68%). No significant difference was seen in primary bone union (X2 (39) = 3.24, p < .07) with 36% of patients treated with nail retention and 68% of patients treated with nail removal reaching primary union; however, this does trend toward an association. Fewer surgeries performed for infection and complication after initial fixation was positively associated with infection clearance (p < .04, M=4.6, SD=2.13, df=39) and primary union (p < .001, M=4, SD=2, df=38).

Conclusion: Infection clearance seems similarly possible with both nail retention and nail removal strategies, with fewer number of surgeries performed for infection and complication improving the likelihood of infection clearance and bone union. This may suggest that more severe FRI’s are less likely to unite and clear infection. Nail removal may play a role in increasing primary bone union; however, a larger sample size is needed for more definitive assessment.
Periviable Breech Delivery Decision Analysis
Authors: Kirksey Makayla , Tucker Edmonds Brownsyne 
Published: December 2021

Background/Objective: The optimal mode of delivery (MOD) for malpresentation in periviable deliveries (22-24 weeks), remains a source of debate. Neonatal and maternal complications can arise from both vaginal (VD) and cesarean delivery (CD), and the threat of maternal morbidity extends to subsequent pregnancies. It has been difficult to compare these risks while counseling patients about MOD options, so we sought to create a decision tree that maps probable outcomes associated with breech deliveries at 23- and 24-weeks’ gestation, as well as complications posed for subsequent pregnancies.

Methods: An extensive literature review was conducted to identify risk estimates of periviable maternal and neonatal outcomes, along with elective repeat CD (ERCD) and trial of labor after cesarean (TOLAC) for subsequent pregnancies. Probabilities were inputted into TreeAge software, starting with primary maternal health states that may result from CD and VD – “death”, “hysterectomy”, or “no hysterectomy”, followed by the probability of neonatal health states– “death”, “severe morbidity”, or “no severe morbidity”. The likelihood of placenta previa or normal placenta was considered for subsequent pregnancies. We factored in the possibility of ERCD or TOLAC and the associated maternal and neonatal risks for each.

Results: Final design of the tree is complete and risk estimates have been inputted. Primary analysis and sensitivity analyses are planned for August 2021. Ultimately, we will also be able to use measured utility values to calculate quality adjusted life years (QALYs) for each health state.

Conclusion and Clinical Impact: Whether CD or VD is optimal for breech presentation in periviable delivery is influenced by a complex array of factors, including future reproductive plans and maternal values related to potential neonatal and maternal morbidity and mortality. Quantifying risks associated with each MOD will aid providers in their efforts to help families make informed decisions and reduce morbidity across the reproductive lifespan.
The Roll of Toll-like Receptors in the Stem Cell Inflammatory Response
Authors: Lawson Katelyn , Markel Troy 
Published: December 2021

Background: Toll-like receptors serve as ligands for LPS and other inflammatory mediators. Previous studies have shown that TLR4 is deleterious, while TLR9 is beneficial in the setting of inflammation and ischemia. Umbilical stem cells (USCs) have shown promise in the acute treatment of inflammation but their response to inflammatory mediators has not been fully elucidated. We hypothesized that knockdown of TLR4 in USCs would result in lower levels of IL-6 and VEGF production, while knockdown of TLR9 would produce higher levels of these cytokines when cells were exposed to LPS or hypoxia.

Methods: USCs were cultured in polystyrene flasks in Mesenpro media at 37C in 5% CO2 in air. Cells were plated into 12-well plates at a concentration of 100,000 cells/well. Cells were transfected for 24h with siRNA to knockdown TLR4 and TLR9, respectively. Knockdown was confirmed by PCR. Experimental groups were: 1) Control, 2) Scramble siRNA for negative control, 3) TLR4 siRNA and 4) TLR9 siRNA. After 24 hours the media was changed and cells were exposed to either LPS (200ng/ml) or 5% oxygen for 24 hours. The supernatant was then collected and analyzed with ELISA for VEGF and IL-6. Data were analyzed by Mann Whitney test and p<0.05 was significant.

Results: TLR4 and TLR9 were effectively knocked down by the transfection process. However, no significant levels of VEGF or IL-6 were detected from any of the experimental groups.

Conclusion and Implications: Although no significant levels of VEGF or IL-6 were detected in the ELISA after exposure to inflammatory agents, there is still indication that TLR4 and TLR9 play critical roles in inflammation. The experiment should be run again and tested for more inflammatory cytokines. Positive results from this study can translate to an in vivo model, in which engineered TLR4KD stem cells have the potential to minimize tissue inflammation and beyond standard cell therapy.

Acknowledgement: This project was funded, in part, with support from the Indiana Clinical and Translational Sciences Institute funded, in part by UL1TR002529 from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The PUMS Database: A Corpus of Previously-Used Musical Stimuli in 306 Studies of Music and Emotion
Authors: Warrenburg Lindsay 
Published: December 2021

A corpus of Previously-Used Musical Stimuli (PUMS) is presented. The PUMS database is an online, publicly-available database where researchers can find a list of 22,417 musical stimuli that have been previously used in the literature on how music can convey or evoke emotions in listeners. A total of 306 studies on music and emotion are included in the database. Each musical stimulus used in these studies was coded according to various criteria: its designated emotion and how it was operationalized, its length, whether it is an excerpt from a longer work, and its style or genre. In the PUMS corpus, there is also information regarding the familiarity of the original participants with each musical sample, as well as information regarding whether each passage was used in a study about perceived or induced emotion. The name of the passage, composer, track number, and specific measure numbers or track location were noted when they were identified in the original paper. The database offers insight into how music has been used in psychological studies over a period of 90 years and provides a resource for scholars wishing to use music in future behavioral or psychophysical research. The PUMS database can be accessed online at https://osf.io/p4ta9.
MeloSol Corpus
Authors: Baker David John 
Published: December 2021

This data report introduces the MeloSol corpus, a collection of 783 Western, tonal monophonic melodies. I first begin by describing the overall structure of the corpus, then proceed to detail its contents as they would be helpful for researchers working in the field of computational musicology or music psychology. In order to contextualize the MeloSol corpus in relation to other corpora in the literature, I present descriptive statistics of the MeloSol corpus alongside the The Densmore Collection of Native American Song and The Essen Folk Song Collection. I suggest possible future uses of this corpus including extending research investigating Western tonality, perceptual experiments needing novel ecological stimuli, or work involving the musical generation of monophonic melodies in the style of Western tonal music.
Como e o que escrever em um relatório científico de pesquisa empírica?
Authors: Faleiros Pedro Bordini , Ferreira André Luíz 
Published: December 2021

Objetivo. O objetivo do presente artigo é fornecer ao leitor instruções, derivadas da literatura científica, sobre o que e como escrever cada seção de um relatório científico de uma pesquisa empírica. Desenvolvimento. Este trabalho está dividido de acordo com a estrutura de um relatório científico (introdução, objetivo, método, resultados e discussão e considerações finais), no qual em cada um dos tópicos são explorados o que deve ser escrito, como e a sequência de tal descrição. Implicações. Acreditamos que este tipo de artigo pode contribuir com alunas e alunos de cursos de graduação que precisam lidar com as dificuldades relacionadas ao bloqueio de escrita acadêmica. How and what to write in a scientific report of empirical research?Abstract:Objective. The purpose of this article is providing the reader with instructions, based on the scientific literature, on how and what to write in each section and topic of a scientific report of empirical research. Development. The article is divided according to the structure of a scientific report (introduction, objective, methods, results and discussion and final considerations), in which each topic explores what should be written, how and the sequence for such description. Implications. We believe that this type of article can contribute to undergraduate students who need to deal with difficulties related to academic writing block.Keywords. Writing block; Scientific report model; Scientific writing.
Krwawy sejmik w Szadku przed sejmem 1623 roku
Authors: Opaliński Edward 
Published: December 2021

Główny sejmik województwa sieradzkiego, który odbywał się w Szadku, uchodził za spokojny i wolny od zakłóceń. Niemniej 13 grudnia 1622 r. Szadek stał się miejscem krwawych wydarzeń. Podobno w przeddzień sejmu przybył do Szadku starosta piotrkowski Olbracht Starołęski w asyście ok. 300 mężczyzn, zajmując ze swoją świtą karczmy, które zwyczajowo służyły za nocleg innym uczestnikom sejmu. Następnego dnia wybuchł konflikt o wspomniane karczmy między częścią szlachty a starostą. Próby udobruchania obu stron podjęli się synowie i siostrzeńcy wojewody sieradzkiego Stanisława Bykowskiego. Strzały nieoczekiwanie oddane przez zwolenników Starołęskiego odebrały życie dwóm członkom rodziny Bykowskich. Rozeźlona szlachta szturmowała kwaterę starosty, zabijając dwóch jego ludzi. Starołęskiego uratowała interwencja posła królewskiego i marszałka sejmiku, przy czym wojewoda sieradzki zachował neutralne stanowisko. Krwawy incydent był konfliktem między zwolennikami króla, spowodowanym bardzo niefortunnym zbiegiem okoliczności.
Autor artykułu podjął próbę odtworzenia przebiegu krwawego zajścia w Szadku na podstawie materiałów przechowywanych w sztokholmskim Riksarkivet (korespondencji oraz dokumentów dotyczących podpisanego w 1623 pojednania), a także innych źródeł zgromadzonych w Archiwum Głównym Akt Dawnych.
Rating of the limiting state of 20K steel under low-cycle loading using the using the method of LM-hardness
Authors: Bulakh P. , Shvets Volodymyr , Maslo О. 
Published: December 2021

In the express diagnostics of the condition of the material of the elements of the structures in operation and the materials for the manufacture of the responsible elements of the equipment, it is necessary to take into account the current damage. At the same time, non-destructive methods of control are more relevant, which allow to assess the level of scattered damage in the material and the degree of degradation of its structure of the metal. Another important factor in controlling
the current state of the material is the assessment of the residual life of the structural element under operating loads. This paper presents the results of research on the application of the non-destructive method of LM-hardness in assessing the characteristics of the limiting state of the material of real structural elements operating under low-cycle load.
Metrological provision of measurement using NanoScan-3D microscopenanothercomer
Authors: Denysiuk Viktor , Symonyuk Volodymyr , Lapchenko Yurii , Tymoshchuk Anton , Cherniak Sofiia 
Published: December 2021

A review of methods and instrumentation for the study of tribological and mechanical properties of the surface. It is established that the most common methods for studying these properties in the micro and nano-ranges are contact methods based on the interaction of the hard tip with the material under study. In scanning probe microscopy, a bimorph piezoceramic probe with a diamond tip is the main element of the measurement modules used for nanoindentation and sclerometry to comprehensively study the tribological and mechanical properties of the surface. The original design of the sensor allows to implement more than ten measuring techniques on one device. The operation of these devices in the semi-contact scanning probe-microscopic mode allows to obtain images of the surface relief and a map of the distribution of elastic properties. The indentation mode allows to measure hardness and modulus of elasticity, to estimate elastic
recovery of material after indentation. The method allows to determine the abrasion resistance and hardness of the material, adhesion and thickness of thin coatings.
Implementation of the specialty "Materials Science" at Kherson National Technical University as an integrated educational and scientific program in the field of mechanical engineering
Authors: Dmitriev Dmytro , Rozov Yurii , Seliverstov Igor 
Published: December 2021

Kherson National University of Technology has introduced an educational and scientific program. Scientific program for the specialty of material sciences and accumulated experience in its provision, taking into account the peculiarities of educational sphere "Mechanical Engineering", educational activity at the previous levels of education (bachelor's degree, master's degree), the requirements of Ukrainian industry and the region in particular. The educational and scientific programs of educational and scientific institutions of Ukraine are analyzed according to their scope, subject area, teaching methods, tools and equipment used. The structure of general and personal competencies and their corresponding program learning outcomes with the necessary educational components of the normative and selective parts are given. It is shown how the value of this or that component is reevaluated by the maintenance group and the external influence on their content by stakeholders of different levels. It is noted that the subject area of educational and scientific programs of the specialty of material sciences covers several areas of different branches of education, which makes it possible to expand its scope to the study of unique phenomena, traditionally do not involve
purely structural studies of materials and goods.