bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proximate Composition, Microbiological Quality and Safety of Raw and Open Sun‐Dried Fish Products in Lake Tana, Ethiopia
Authors: Birie Solomon , Mingist Minwyelet , Kibret Mulugeta , Atlog Tadlo Yitayew , Geremew Hirut , Getnet Banchiamlak 
Published: December 2024

ABSTRACTThe quality and safety of fish products are crucial because poorly handled fish products can result in foodborne illnesses, spoilage, and economic losses. Data on the nutritional and microbiological quality of fish products in Ethiopia, especially in Lake Tana, is limited. This study assessed the proximate composition and microbial quality of raw and open sun‐dried fish products in Lake Tana. Using the standard procedures, 60 fish tissue samples were collected and analyzed for proximate composition and microbial quality. The open sun‐dried fish had significantly higher ash (10.08% ± 1.98%), fat (6.01% ± 1.59%), protein (48.76% ± 8.55%), water activity (0.7358 ± 0.0368) and salt contents (5.89% ± 3.17%) compared to the raw fish (p < 0.05). A significant difference was observed between raw and dried fish in aerobic mesophilic bacteria, Staphylococcus aureus, yeast and mold counts (p < 0.05). The mean total and fecal coliform counts were not significantly different between the raw and dried fish (p > 0.05). In raw fish, 16.6% and 83.3% of samples had total coliforms and fecal coliforms exceeding the recommended limits, respectively. Both raw and dried fish samples tested positive for Salmonella and Shigella spp. There was a strong positive correlation between water activity and microbial load (r = 0.756, p < 0.05) and also between moisture content and microbial load (r = 0.786, p < 0.05). Most of the assessed raw and sun‐dried fish samples were above the international microbial specification limits, which need attention to ensure the safety of fish consumers.
Comprehensive lung microbial gene and genome catalogs assist the mechanism survey of Mesomycoplasma hyopneumoniae strains causing pig lung lesions
Authors: Li Jingquan , Huang Fei , Zhou Yunyan , Huang Tao , Tong Xinkai , Zhang Mingpeng , Chen Jiaqi , Zhang Zhou , Du Huipeng , Liu Zifeng , Zhou Meng , Xiahou Yiwen , Ai Huashui , Chen Congying , Huang Lusheng 
Published: December 2024

AbstractUnderstanding the community structure of the lower respiratory tract microbiome is crucial for elucidating its roles in respiratory tract diseases. However, there are few studies about this topic due to the difficulty in obtaining microbial samples from both healthy and disease individuals. Here, using 744 high‐depth metagenomic sequencing data of lower respiratory tract microbial samples from 675 well‐phenotyped pigs, we constructed a lung microbial gene catalog containing the largest scale of 10,031,593 nonredundant genes to date, 44.8% of which are novel. We obtained 356 metagenome‐assembled genomes (MAGs) which were further clustered into 256 species‐level genome bins with 41.8% being first reported in the current databases. Based on these data sets and through integrated analysis of the isolation of the related bacterial strains, in vitro infection, and RNA sequencing, we identified and confirmed that Mesomycoplasma hyopneumoniae (M. hyopneumoniae) MAG_47 and its adhesion‐related virulence factors (VFs) were associated with lung lesions in pigs. Differential expression levels of adhesion‐ and immunomodulation‐related VFs likely determined the heterogenicity of adhesion and pathogenicity among M. hyopneumoniae strains. M. hyopneumoniae adhesion activated several pathways, including nuclear factor kappa‐light‐chain‐enhancer of activated B, mitogen‐activated protein kinase, cell apoptosis, T helper 1 and T helper 2 cell differentiation, tumor necrosis factor signaling, interleukin‐6/janus kinase 2/signal transducer and activator of transcription signaling, and response to reactive oxygen species, leading to cilium loss, epithelial cell‒cell barrier disruption, and lung tissue lesions. Finally, we observed the similar phylogenetic compositions of the lung microbiome between humans with Mycoplasma pneumoniae and pigs infected with M. hyopneumoniae. The results provided important insights into pig lower respiratory tract microbiome and its relationship with lung health.
Predictive Maintenance for UWSNs: A Practical Implementation of Fault Detection Using NDN and Deep Learning
Authors: Benarfa Abdelmadjid , Dahmane Sofiane , Brik Bouziane , Yagoubi Mohamed Bachir 
Published: December 2024

ABSTRACTUnderwater Wireless Sensor Networks (UWSNs) play a critical role in collecting data for various marine applications, but their deployment and maintenance in harsh underwater environments pose significant challenges. Building upon our prior conceptual work, this article presents a practical implementation of an AI‐driven fault detection system for UWSNs, a key component of a future predictive maintenance framework incorporating Named Data Networking (NDN). Using the “Underwater Sensor Dataset,” we implemented a feed‐forward neural network to classify sensor readings as healthy or faulty. The model achieved high accuracy (99.9%), precision (100%), recall (99.1%), and F1‐score (99.6%) on the test set, demonstrating its effectiveness in fault detection. Our findings highlight the feasibility and potential of AI‐based predictive maintenance in UWSNs, even with a simpler neural network model. The framework promises reduced downtime, minimized maintenance costs, and an extended lifespan for UWSNs.
Lysosphingolipid Quantitation in Plasma and Dried‐Blood Spots Using Targeted High‐Resolution Mass Spectrometry
Authors: Ducatez Franklin , Mauhin Wladimir , Ottaviani Jules , Plichet Thomas , Pilon Carine , Lidove Olivier , Barbey Fréderic , Perrichot Régine , Vergnaud Sabrina , Berger Marc G. , Berger Juliette , Belmatoug Nadia , Nadjar Yann , Lamari Foudil , Noel Esther , Marret Stéphane , Bekri Soumeya , Tebani Abdellah 
Published: December 2024

ABSTRACTBackgroundSphingolipidoses are rare inherited metabolic diseases belonging to lysosomal diseases. Early and accurate diagnosis is crucial for effective management and treatment. In this study, we aimed to develop a robust method to accelerate the diagnosis of these sphingolipidoses using dried blood spots and plasma.MethodWe employed high‐resolution mass spectrometry coupled with liquid chromatography (LC‐HRMS) to analyze 6 lysosphingolipids (GlcSph/Psychosine, LysoGb3, LysoSM, LysoSM509, LysoGM1, and LysoGM2) on dried blood spots and plasma samples. The method was used to measure the lysosphingolipid levels in a group of 30 control subjects and 204 samples from patients with sphingolipidoses (61 dB and 143 plasma) including Fabry, Gaucher, GM2 Gangliodosis, Niemann‐Pick type A/B, and Niemann‐Pick type C.ResultsThe developed multiplex LC‐HRMS method demonstrated linearity, precision, and quantification performances particularly for GlcSph/Psychosine and LysoGb3 on samples including controls and patients with sphingolipidoses. LysoSM showed recovery variability, wherease LysoGM1 and LysoGM2 showed higher matrix effect.ConclusionOur study presents a high‐resolution mass spectrometry method along with the established cutoff values, providing a valuable tool for targeted screening, accurate diagnosis, and monitoring sphingolipidoses. Furthermore, DBS showed reliable results that lay the path to a broader adoption for screening these diseases.
Severe Impact of Omeprazole Timing on pH‐Sensitive Dasatinib Absorption: Unveiling Substantial Drug–Drug Interaction
Authors: Andersson Per , Brisander Magnus , Liljebris Charlotta , Jesson Gérald , Lennernäs Hans 
Published: December 2024

AbstractThe absorption and bioavailability of most tyrosine kinase inhibitors are affected by gastrointestinal pH as they are weak basic lipophilic drugs. Hence, concomitant use of acid reducing agents (ARAs) is frequently restricted. Particularly comedication of crystalline dasatinib (Sprycel) and proton‐pump inhibitors (PPIs) should be avoided. Drug–drug interaction (DDI) studies with PPIs report approximately 40%‐80% bioavailability reduction of dasatinib. Limitations in the design of these studies do not allow for assessing the near maximum DDI as timing of PPI dosing was either not reported or 22 h prior to dasatinib intake. We conducted a DDI study of crystalline dasatinib and omeprazole in healthy, fasted participants, investigating the impact of PPI comedication on dasatinib plasma exposure at a time point when the near maximum DDI effect is expected. Participants were administered omeprazole (day 2‐5) to reach steady state. On day 6, a single dose of crystalline dasatinib was given. Crystalline dasatinib dosing alone on day 1 served as control (single dose). The dosing interval between omeprazole administration and crystalline dasatinib was 10 h (median [range: 9‐10 h]). Dasatinib Cmax and AUC0‐24 were reduced by 96% and 89% by omeprazole comedication. Cmax was 224.6 ± 104.7 ng/mL (mean ± SD) and 8.0 ± 4.5 ng/mL (P < .0001) and AUC0‐24 was 797.6 ± 274.5 and 90.6 ± 38.1 h·ng/mL (P < .0001) without and with omeprazole. T1/2 was 5.7 ± 1.5 h (mean ± SD) with crystalline dasatinib dosing alone and could not be reliably calculated with comedication. To ensure optimal patient outcome, it is vital to investigate bioavailability of pH‐sensitive drugs at the maximal DDI effect of ARAs to understand the worst‐case influence for efficient clinical management.
Microscopic Observation and Physicochemical Analysis Reveal the Microstructure and Chemical Composition of Abelmoschus manihot Flower and Its Potential Value in Anti‐Caries Applications
Authors: Sun Zenghong , Wang Linyang , Chen Pin , Tian Shuge , Chen Yan 
Published: December 2024

ABSTRACTAbelmoschus manihot flower (AMF), commonly cultivated in Southern China, is homology of medicine and food. In this study, microscopic observation revealed the microstructure of AMF, including upper epidermal cells and nonglandular hairs that play roles in defense and water management. Physicochemical analyses indicated that AMF powder exhibits weak acidity and low moisture content, suggesting its stability. Further fluorescence and spectroscopic analyses identified a diverse array of chemical constituents in AMF, including various flavonoids. Using ultra high performance liquid chromatography_quadrupole exactive hybrid mass spectrometer (UHPLC‐Q Exactive HFX) technology, a total of 407 compounds were identified in AMF, 79 of which were found to have a relative content exceeding 0.1%. A network pharmacological analysis identified 31 potential active components and delineated 10 core targets associated with dental caries. Molecular docking studies demonstrated that the selected active components exhibited strong binding affinities with the aforementioned core targets, particularly flavonoids such as quercetin and morin. In conclusion, this study provides a comprehensive analysis of AMF's microstructure and chemical composition, establishing a scientific foundation for its applications in the pharmaceutical and food industries, as well as in oral health for caries prevention, while also exploring its potential mechanisms of action.
A Comparative Study of Near‐Surface Wind Speed Observations and Reanalysis Datasets in China Over the Past 38 Years
Authors: Mi Xiyuan , Liu Peng 
Published: December 2024

ABSTRACTNear‐surface wind speed (NSWS) reanalysis products have been widely used in NSWS research, yet there is a lack of evaluation for NSWS reanalysis products. Based on the 10 m wind speed observations data from stations during 1980–2017, this article evaluated the climatic characteristics of NSWS over mainland China at various spatiotemporal scales using four reanalysis datasets: ERA5, MERRA‐2, NCEP‐2 and JRA‐3Q. Compared with station observations, ERA5 and NCEP‐2 showed positive biases in the climatology of NSWS, MERRA‐2 showed negative biases and JRA‐3Q showed significantly negative biases. Regarding the spatial distribution of the climatology, ERA5 performed the best with a spatial correlation coefficient of 0.55 with stations. At the interannual scale, ERA5 showed the best performance with a correlation coefficient of 0.68 with stations, significantly better than the other three reanalysis datasets. At the interdecadal scale, MERRA‐2, NCEP‐2 and JRA‐3Q can generally reproduce the decreasing trend in NSWS from 1980 to 2011. However, none of the four reanalysis datasets can capture the increasing trend from 2011 to 2017. In terms of the annual cycle, only ERA5 can well reproduce the seasonal characteristics in different regions, including the maximum NSWS in the north and the Qinghai–Tibet Plateau in spring and the south of the Yangtze River in summer. Considering the evaluation results at different spatiotemporal scales, ERA5 exhibited good performance regarding the spatial distribution of climatology, interannual variability and annual cycle, but failed to reproduce observational features at the interdecadal scale. JRA‐3Q showed significant advantages in terms of interdecadal variability, whereas neither MERRA‐2 nor NCEP‐2 showed prominent advantages in various aspects.
Increasing Safe Harbor Agreement enrollment: a choice experiment approach to investigate preferred contract attributes
Authors: Rubino Elena C. , Messick Jared A. , Serenari Christopher 
Published: December 2024

AbstractSafe Harbor Agreements (SHAs) are one of the voluntary incentive programs designed to neutralize some of the costs and burdens associated with endangered species protection on private lands under the Endangered Species Act. They have demonstrated mixed success in terms of landowner participation, indicating that merely offering an SHA is not enough to persuade landowners to enter into agreements with government entities to protect endangered species. Yet little research has investigated landowner decision‐making and the programmatic structures that influence participation in SHAs. In this study, we employed hypothetical choice experiment offerings of Houston toad (Bufo houstonensis) SHAs to explore the reasoning behind Texas landowners' (n = 956) willingness to participate in the program, and to reveal programmatic characteristics most appealing to landowners. Respondents were most frequently willing to enroll in an SHA because of beliefs about and a sense of responsibility towards conserving nature, and were unwilling to potentially enroll in an SHA because they did not know enough about SHAs, did not want the government involved in their land management, and did not want to enter a binding agreement regarding endangered species. Additionally, respondents were more likely to enroll in hypothetical SHAs if the agreements included 10‐year contract durations and full future assurances, and less likely to enroll if they included 30‐year contract durations. This study provides program administrators with insights into how to improve the design and delivery of SHAs to increase participation among private landowners.
Influence of the Sex of Translocation Carrier on Clinical Outcomes of Couples Undergoing Preimplantation Genetic Testing
Authors: Zhang Zhiping , Chen Jiayao , Zhang Lei , Wei Ruiyang , Liu Zhen , Zhao Dunmei , Bi Xingyu , Liang Lixia , Zhang Xueluo , Su Dan , Wu Xueqing 
Published: December 2024

ABSTRACTIntroductionThis study investigated the impact of the carrier on transferable blastocyst rate and live birth outcomes in couples with structural chromosomal abnormalities.MethodsCouples were grouped into reciprocal translocation, Robertsonian translocation, or inversions groups, and clinical data were retrospectively analyzed. Preimplantation genetic testing for chromosomal structural rearrangements (PGT‐SR) was conducted, and pregnancy outcomes were compared.ResultsEmbryo euploidy rates between male and female carriers differed nonsignificantly in the reciprocal translocation and inversion groups but significantly among the three groups. In the Robertsonian translocation group, male carriers had a higher embryo euploidy rate than that of female carriers. Sperm density of male carriers with reciprocal and Robertsonian translocation was significantly lower than that of the female carrier in the same group, whereas the difference was non‐significant in the inversion group. The clinical outcomes of embryo transfer post‐PGT were similar regardless of the sex of carriers with structural chromosomal abnormalities. Female carriers with Robertsonian translocations displayed a higher risk of producing embryos with chromosomal abnormalities than male carriers. Pregnancy, live birth, and cumulative live birth rates following a PGT‐SR cycle were similar, irrespective of chromosomal structural rearrangement type, and carrier sex.ConclusionOur findings offer valuable information for genetic counseling.
Copper, Zinc, and Titanium‐Based Semiconductor Nanomaterials for Antimicrobial Coatings and Their Mechanisms
Authors: Manisekaran Ravichandran , Chettiar Aruna‐Devi Rasu , Marasamy Latha , Arthikala Manoj‐Kumar , Kandasamy Ganeshlenin , Ibarra Verónica Campos , Rathore Hanumant Singh 
Published: December 2024

ABSTRACTThroughout the course of human civilization, numerous disease outbreaks have caused major impacts by affecting the lives of mankind. Currently, excessive and improper use of antimicrobials has led to the emergence of drug‐resistant pathogens, making Antimicrobial Resistance (AMR) a critical global threat, as recognized by the World Health Organization (WHO). If left unaddressed, it will cost approximately USD 100 trillion to the world economy by 2050. Therefore, nanotechnology has emerged as a promising field, offering a wide range of nanomaterials that can be effectively utilized to combat microorganisms. Among these materials, semiconductor nanomaterials (SNMs) have attracted significant attention owing to their exceptional inherent properties that can be effectively employed. Currently, hospital‐acquired infections and infections associated with medical devices pose substantial risks to patients. Consequently, researchers have explored the application of SNMs in the form of antimicrobial coatings or surfaces. This review presents a comprehensive and recent investigation of widely studied SNMs, such as titanium, zinc, and copper‐based, for coating various surfaces (glass, stainless steel, medical devices, textiles, and polymers) and their antimicrobial effects. By preventing or eradicating microbial growth, SNMs have the potential to exert a positive impact, offering a viable solution for coating essential and industrial surfaces.
Efficacy of antiobesity medications for weight reduction in older adults: a systematic review
Authors: Chen Alissa S. , Hajduk Alexandra M. , Grimshaw Alyssa A. , Fried Terri R. , Jastreboff Ania M. , Lipska Kasia J. 
Published: December 2024

AbstractObjectiveThe objective of this study was to examine weight reduction and adverse events associated with use of antiobesity medications (AOMs) in older adults ages ≥65 years.MethodsSeven databases were searched for studies evaluating weight reduction of Food and Drug Administration (FDA)‐approved AOMs. Studies had to include adults ages ≥65 years with obesity (BMI ≥ 30 kg/m2 or ≥27 kg/m2 with one weight‐related condition), with independent analysis of weight reduction for adults ages ≥65 years. Two coauthors extracted and evaluated studies for risk of bias using standardized forms.ResultsSix experimental studies (five secondary analyses of randomized clinical trial data and one single‐arm trial) and two observational studies met inclusion criteria. Seven medications were studied. Sample size of older adults ranged from 13 to 6728. Experimental studies predominantly included patients with concurrent prediabetes or cardiovascular disease. All studies found statistically significant weight reduction between intervention and placebo groups or compared with baseline weight. Few studies reported on adverse events.ConclusionsLimited evidence suggests weight reduction of AOMs in older adults, with the best current evidence for the use of semaglutide in older adults with obesity and cardiovascular disease. Larger, more inclusive studies of older adults are needed to guide clinical care and determine the tolerability of AOMs for older adults.
Analysis of the Serum MicroRNA Expression Profiles in Patients With Type 2 Diabetic Nephropathy After Simultaneous Pancreas‐Kidney Transplantation
Authors: Liu Luhao , Yu Chaoqun , Zhang Peng , Chen Rongxin , Guo Yuhe , Fang Jiali , Ma Junjie , Li Guanghui , Chen Zheng 
Published: December 2024

ABSTRACTBackgroundSimultaneous pancreas‐kidney (SPK) transplantation has become a standard therapy for end‐stage diabetic nephropathy patients. This study aims to investigate serum microRNA (miRNA) profiles in patients with type 2 diabetic nephropathy after SPK and explored the mechanism of insulin resistance induced by relevant miRNAs.MethodsPatients with type 2 diabetic nephropathy were recruited at our organ transplantation center from June 2018 to March 2019. Six patients were randomly selected, and miRNA microarray analysis was performed to assess serum miRNA expression levels. Target genes were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was conducted.ResultsThe gene chip results revealed significant upregulation of miR‐320d, miR‐4701‐3p, and miR‐940 and significant downregulation of miR‐1229‐5p, miR‐125a‐3p, miR‐3614‐5p, miR‐6090, and miR‐6893‐5p. Target gene prediction and pathway analysis revealed that four miRNAs (miR‐125a‐3p, miR‐320d, miR‐940, and miR‐1229‐5p) form distinct signal regulatory networks.ConclusionSpecific miRNA expression profiles are present in the serum of patients with diabetic nephropathy after simultaneous pancreas‐kidney transplantation. These specific miRNAs may be involved in the regulatory mechanism of restoring normoglycemia in diabetic nephropathy patients.
Cellulose‐Based Transparent Superhydrophobic Coatings With a Four‐Layer “Armor” Structure for Anti‐Fouling and Anti‐Icing Applications
Authors: Deng Li , Zhang Weiliang , Zhang Tiantian , Huang Yawen , Hu Chengyao 
Published: December 2024

ABSTRACTA superhydrophobic transparent coating effectively inhibits icing and promotes self‐cleaning, rendering it exceptionally valuable for practical applications in solar panels and car windows. However, attaining both hydrophobicity and transparency in superhydrophobic coatings continues to pose a significant challenge. Thus, this study introduces an innovative four–layered structure. The bottom layer was treated with hyperbranched epoxy–oligosiloxane nanocluster (HP) to serve as a binder. The intermediate layer was modified with amino–silane–modified nano–cellulose (APT–CNF) to develop a micro–scale skeletal structure. The granular layer incorporated fluorine‐modified dendritic silica nanochains (FNPs) to induce nano–structural roughness, while the surface layer employed a silicon elastomer polymer (BFVSE) as an armor‐protective coating featuring both micro– and nano–structural characteristics. The coating exhibits remarkable superhydrophobic properties, with a CA measured at approximately 155°. Furthermore, it demonstrates exceptional light transmittance, reaching approximately 93.1% at a wavelength of 545.5 nm, in contrast to around 91.4% for uncoated glass. The transparent superhydrophobic coating retains its superhydrophobic properties over extended periods following exposure to water impact, sand impact, mechanical bending, high‐temperature heat treatment, acid–base impregnation, and ultraviolet irradiation. Subsequent to dust accumulation testing, the coating continues to exhibit high optical transparency. Its superior performance in solar energy applications, among other fields, indicates significant potential for practical applications.