bluuebunny/crossref_metadata_2025_split huggingface dataset:
EPAC2 acts as a negative regulator in Matrigel-driven tubulogenesis of human microvascular endothelial cells
Authors: Ikeda Takayuki , Yoshitake Yoshino , Yoshitomi Yasuo , Saito-Takatsuji Hidehito , Ishigaki Yasuhito , Yonekura Hideto 
Published: September 2021

AbstractAngiogenesis is physiologically essential for embryogenesis and development and reinitiated in adult animals during tissue growth and repair. Forming new vessels from the walls of existing vessels occurs as a multistep process coordinated by sprouting, branching, and a new lumenized network formation. However, little is known regarding the molecular mechanisms that form new tubular structures, especially molecules regulating the proper network density of newly formed capillaries. This study conducted microarray analyses in human primary microvascular endothelial cells (HMVECs) plated on Matrigel. TheRAPGEF4gene that encodes exchange proteins directly activated by cAMP 2 (EPAC2) proteins was increased in Matrigel-driven tubulogenesis. Tube formation was suppressed by the overexpression of EPAC2 and enhanced by EPAC2 knockdown in endothelial cells. Endothelial cell morphology was changed to round cell morphology by EPAC2 overexpression, while EPAC2 knockdown showed an elongated cell shape with filopodia-like protrusions. Furthermore, increased EPAC2 inhibited endothelial cell migration, and ablation of EPAC2 inversely enhanced cell mobility. These results suggest that EPAC2 affects the morphology and migration of microvascular endothelial cells and is involved in the termination and proper network formation of vascular tubes.
Functional characterization and structural bases of two class I diterpene synthases in pimarane-type diterpene biosynthesis
Authors: Xing Baiying , Yu Jiahui , Chi Changbiao , Ma Xueyang , Xu Qingxia , Li Annan , Ge Yuanjie , Wang Zhengdong , Liu Tan , Jia Hongli , Yin Fuling , Guo Juan , Huang Luqi , Yang Donghui , Ma Ming 
Published: September 2021

AbstractPimarane-type diterpenoids are widely distributed in all domains of life, but no structures or catalytic mechanisms of pimarane-type diterpene synthases (DTSs) have been characterized. Here, we report that two class I DTSs, Sat1646 and Stt4548, each accept copalyl diphosphate (CPP) as the substrate to produce isopimara-8,15-diene (1). Sat1646 can also accept syn-CPP and produce syn-isopimaradiene/pimaradiene analogues (2–7), among which 2 possesses a previously unreported "6/6/7" ring skeleton. We solve the crystal structures of Sat1646, Sat1646 complexed with magnesium ions, and Stt4548, thereby revealing the active sites of these pimarane-type DTSs. Substrate modeling and subsequent site-directed mutagenesis experiments demonstrate different structural bases of Sat1646 and Stt4548 for 1 production. Comparisons with previously reported DTSs reveal their distinct carbocation intermediate stabilization mechanisms, which control the conversion of a single substrate CPP into structurally diverse diterpene products. These results illustrate the structural bases for enzymatic catalyses of pimarane-type DTSs, potentially facilitating future DTS engineering and combinatorial biosynthesis.
Pervasive detachment faults within the slow spreading oceanic crust at the poorly coupled Antilles subduction zone
Authors: Marcaillou Boris , Klingelhoefer Frauke , Laurencin Muriel , Lebrun Jean-Frédéric , Laigle Mireille , Lallemand Serge , Schenini Laure , Gay Aurélien , Boucard Milton , Ezenwaka Kingsley , Graindorge David 
Published: September 2021

AbstractOceanic crust formed at slow-spreading ridges is currently subducted in only a few places on Earth and the tectonic and seismogenic imprint of the slow-spreading process is poorly understood. Here we present seismic and bathymetric data from the Northeastern Lesser Antilles Subduction Zone where thick sediments enable seismic imaging to greater depths than in the ocean basins. This dataset highlights a pervasive tectonic fabric characterized by closely spaced sequences of convex-up Ridgeward-Dipping Reflectors, which extend down to about 15 km depth with a 15-to-40° angle. We interpret these reflectors as discrete shear planes formed during the early stages of exhumation of magma-poor mantle rocks at an inside corner of a Mid-Atlantic Ridge fracture zone. Closer to the trench, plate bending could have reactivated this tectonic fabric and enabled deep fluid circulation and serpentinization of the basement rocks. This weak serpentinized basement likely explains the very low interplate seismic activity associated with the Barbuda-Anegada margin segment above.
Multi‐view facial action unit detection via deep feature enhancement
Authors: Tang Chuangao , Lu Cheng , Zheng Wenming , Zong Yuan , Li Sunan 
Published: September 2021

AbstractMulti‐view facial action unit (AU) analysis has been a challenging research topic due to multiple disturbing variables, including subject identity biases, variational facial action unit intensities, facial occlusions and non‐frontal head‐poses. A deep feature enhancement (DFE) framework is presented to tackle some of these coupled complex disturbing variables for multi‐view facial action unit detection. The authors' DFE framework is a novel end‐to‐end three‐stage feature learning model with taking subject identity biases, dynamic facial changes and head‐pose into consideration. It contains three feature enhancement modules, including coarse‐grained local and holistic spatial feature learning (LHSF), spatio‐temporal feature learning (STF) and head‐pose feature disentanglement (FD). Experimental results show that the proposed method achieved state‐of‐the‐art recognition performance on the FERA2017 dataset. The code is released at http://aip.seu.edu.cn/cgtang/.
Detecting exoplanets with the false inclusion probability
Authors: Hara Nathan C. , Unger Nicolas , Delisle Jean-Baptiste , Díaz Rodrigo F. , Ségransan Damien 
Published: September 2021

Context.It is common practice to claim the detection of a signal if, for a certain statistical significance metric, the signal significance exceeds a certain threshold fixed in advance. In the context of exoplanet searches in radial velocity data, the most common statistical significance metrics are the Bayes factor and the false alarm probability (FAP). Both criteria have proved useful, but do not directly address whether an exoplanet detection should be claimed. Furthermore, it is unclear which detection threshold should be taken and how robust the detections are to model misspecification.Aims.The aim of the present work is to define a detection criterion that conveys as precisely as possible the information needed to claim an exoplanet detection, as well as efficient numerical methods to compute it. We compare this new criterion to existing ones in terms of sensitivity and robustness to a change in the model.Methods.We define a general detection criterion called the false inclusion probability (FIP). In the context of exoplanet detections it provides the posterior probability of presence of a planet with a period in a certain interval. Posterior distributions are computed with the nested sampling packagePOLYCHORD. We show that for FIP and Bayes factor calculations, defining priors on linear parameters as Gaussian mixture models can significantly speed up computations. The performance of the FAP, Bayes factor, and FIP are studied via simulations and analytical arguments. We compare the methods assuming the model is correct, then evaluate their sensitivity to the prior and likelihood choices.Results.Among other properties, the FIP offers ways to test the reliability of the significance levels; it is a particularly efficient way to account for aliasing, and it allows the presence of planets to be excluded with a certain confidence. In our simulations, we find that the FIP outperforms existing detection metrics. We show that low amplitude planet detections are sensitive to priors on period and semi-amplitude, which will require further attention for the detection of Earth-like planets. We recommend to let the parameters of the noise model free in the analysis, rather than fixing a noise model based on a fit to ancillary indicators.
Einsatz eines Wendelappens zur Wiederherstellung des Volumens im Achselbereich – ein neuer Behandlungsansatz für die operative Versorgung von sekundären Lymphödemen nach Brustkrebstherapie
Authors: Marx Mario , Geßner Michael , Florek Alexander , Morgenstern Susanne , Passin Stefan , Brucker Sara Y. , Wallwiener Diethelm , Hahn Markus 
Published: September 2021

Zusammenfassung
Einleitung Narbige Verengungen und ein Volumendefizit im Bereich der Axilla können zu einem anhaltenden Lymphödem führen und zählen zu den häufigsten Nebenwirkungen nach einer Brustkrebstherapie im Achselbereich. Ziel dieser Arbeit ist die Vorstellung einer Operationstechnik zur Reduzierung von Lymphödemen mithilfe eines Turn-Over Flaps.
Material und Methoden Zwischen Oktober 2016 und Mai 2018 wurden 5 Patientinnen mit einem Lymphödem Grad 2 nach Mammakarzinomtherapie mit dieser neuen Methode behandelt. Dabei erfolgte die vollständige Entfernung der kompromittierenden Narbenstränge der Axilla, gefolgt von einer Reaugmentation der Axilla mittels Turn-Over Flap. Danach wurde bei allen Patientinnen eine Brustrekonstruktion mit einer an die thorakodorsalen Gefäße angeschlossenen Fernlappenplastik durchgeführt. Prä- und postoperativ wurden der Umfang beider Arme, die Lebensqualität und die Schmerzen gemessen.
Ergebnisse Im Durchschnitt sanken die Schmerzen im ipsilateralen Arm von 6 auf 1 auf der numerischen Schmerzskala, und die Lebensqualität verbesserte sich merklich (2,8 vs. 7,0). Es zeigte sich eine deutliche Verringerung des Lymphödems, vor allem im Oberarm. Komplikationen wie sekundäre Blutungen, Infektionen oder Lappenverlust sind nicht aufgetreten.
Schlussfolgerung Eine vollständige Entfernung der nach Brustkrebsbehandlungen entstandenen Vernarbungen im Bereich der Axilla und eine Reaugmentation der Axilla mittels Turn-Over Flap führten zu einer Verringerung des ipsilateralen Lymphödems und zu einer Verbesserung der Schmerzen und der Lebensqualität. Wir deuten dieses Outcome als Ergebnis der physiologischen Ausrichtung der Lymphgefäße und eines lymphknotenhaltigen Transpositionslappens. Die Reaugmentation der Axilla mittels Turn-Over Flap könnte eine wirksame, nicht mikrochirurgische Alternative zur Lymphknotentransplantation darstellen.
Update Mammakarzinom 2020 Teil 5 – Einführung von Substanzen aus der metastasierten Therapiesituation in die frühe Therapiesituation
Authors: Lux Michael P. , Schneeweiss Andreas , Hartkopf Andreas D. , Müller Volkmar , Janni Wolfgang , Belleville Erik , Stickeler Elmar , Thill Marc , Fasching Peter A. , Kolberg Hans Christian , Untch Michael , Harbeck Nadia , Wöckel Achim , Thomssen Christoph , Schulmeyer Carla E. , Welslau Manfred , Overkamp Friedrich , Schütz Florian , Lüftner Diana , Ditsch Nina 
Published: September 2021

ZusammenfassungBei neuen Therapieansätzen des Mammakarzinoms sind insbesondere bei Patientinnen mit HER2-positivem und HER2-negativem/hormonrezeptorpositivem (HR+) Mammakarzinom in den letzten Jahren deutliche Fortschritte gesehen worden. Bei HER2-positiven Tumoren müssen in dem Zusammenhang Pertuzumab, T‑DM1, Neratinib und bald auch Tucatinib und Trastuzumab-Deruxtecan (beide in Europa noch nicht zugelassen) genannt werden. Bei den Patientinnen mit HER2−/HR+-Mammakarzinom sind insbesondere die CDK4/6-Inhibitoren und der PIK3CA-Inhibitor Alpelisib zu nennen. Weitere, neue Therapien wie Akt-Kinase-Inhibitoren und orale SERDs (selective estrogen receptor down-regulators) werden bereits in laufenden klinischen Studien untersucht. Nicht nur bei den HER2-positiven Tumoren halten die Therapien Einzug in die kurative, (neo-) adjuvante Therapiesituation, sondern es wurde nun eine erste positive Studie mit Abemaciclib in der adjuvanten Situation vorgestellt. Bei Patientinnen mit einem triple-negativen Mammakarzinom ist nach vielen Jahren mit negativen Studienergebnissen mit dem Trop-2-Antikörper-Wirkstoff-Konjugat (antibody drug conjugate, ADC) Sacituzumab-Govitecan eine randomisierte Studie veröffentlicht worden, die einen deutlichen Therapiefortschritt bedeuten kann. Diese Übersichtsarbeit beschreibt die neuesten Entwicklungen beim Mammakarzinom nach dem ESMO-Kongress 2020.
Uretroplastia bulbar con injerto: Una actualización sobre las diferentes técnicas quirúrgicas
Authors: Rodríguez Eduar S. , Serna Laura A. , Agressot Wilmer A. 
Published: September 2021

ResumenEn pacientes con estrechez uretral bulbar de una longitud mayor a 2 cm, que no sean candidatos a otras técnicas, se realiza uretroplastia con injerto. Actualmente se emplean diversas técnicas, cada una con ventajas y desventajas propias.Describir las ventajas y desventajas de las técnicas quirúrgicas empleadas actualmente en la uretroplastia con injerto, así como sus tasas de éxito.Se hizo una búsqueda en PubMed, ClinicalKey y en ScienceDirect, utilizando las palabras claves: “urethral stricture,” “urethroplasty,” “oral graft” y “flap.” Se utilizaron los estudios más relevantes, tanto originales como revisiones sistemáticas y meta-análisis, en inglés y en español.Las diferentes técnicas quirúrgicas ofrecen ventajas y desventajas teóricas frente a las otras, aunque las tasas de éxito en todas es cercana al 90%, sin ser una francamente superior frente a las demás.La elección de la técnica quirúrgica a realizar depende de las preferencias y experiencia del cirujano, dado que la tasa de éxito para todas las técnicas es similar.
Urological Cancer Treatment by a Multidisciplinary Team throughout the Covid-19 Pandemic: What Have We Learned?
Authors: Chavarriaga Julián , Cataño Juan-Guillermo , Villanueva Juliana , Sáenz Daniel , Suso-Palau Daniel , Rodríguez Santiago , Villaquiran Catalina , Galvis Juan , Pinilla Paola , Morales Angélica , Patiño German 
Published: September 2021

Abstract
Introduction It is known that cancer care is best approached by a multidisciplinary team (MDT). This became specifically true in the Covid-19 pandemic in which choices for urological cancer treatment are influenced by many factors. In some cases, delayed treatment may have consequences regarding the patient's oncological outcomes. The aim of the present article is to report our experience throughout the Covid-19 pandemic treating patients with urological neoplasms at a high-volume center.
Methods We used a convenience sampling method. Cases were evaluated and discussed on an individual basis at the MDT meetings, and, after a consensus regarding delaying or scheduling treatment, patients were scheduled according to the risk of postponing the procedures. The Medically Necessary, Time-Sensitive (MeNTS) scoring system was measured in each patient; all patients answered the Centers for Disease Control and Prevention (CDC) Covid-19 self-screening questionnaire prior to surgery. The Covid-19-free survival rate was estimated.
Results A total of 194 patients were assessed by the multidisciplinary team and finally treated, with median follow-up of 4 (interquartile range [IQR]: 2.75 to 6) months. Only two patients had Covid-19 confirmed by real-time polymerase chain reaction (RT-PCR). In total, 54 patients underwent oncological surgery, 129 were treated with radiotherapy, and 11 were treated with intravenous chemotherapy. The median age was 66 years (IQR: 59 to 94 years), and the median MeNTS score in the surgically-treated cohort was 35 points (IQR: 31 to 47 points).
Conclusions The evaluation and treatment of urological cancer should be conducted by an MDT; this is of utmost importance, especially during the Covid-19 pandemic. The data collected in our institution showed that most patients could be safely treated by taking all necessary precautions and discussing each case individually in the MDT meetings and performing a close follow-up.
Síndrome compartimental bilateral de pierna tras posición de litotomía prolongada. Presentación de un caso clínico
Authors: Díez-Albero Luis , Moril-Peñalver Lorena , Lapeña-Martínez Andrés , López-Prats Fernando 
Published: September 2021

Resumen
Introducción El síndrome compartimental es una complicación infrecuente pero severa que puede aparecer ante una posición de litotomía prolongada y el uso de perneras.
Objetivo Presentar un caso de síndrome compartimental bilateral tras posición de litotomía prolongada asociada con el uso de perneras.
Material y Métodos Paciente varón de 43 años sometido a una cirugía urológica en posición habitual de litotomía durante un periodo de 6 horas. A las 2 horas de finalizar la cirugía, el paciente presentó dolor intenso y endurecimiento de compartimento anterior y lateral de ambas piernas, junto con una elevación de la creatina quinasa sérica (valor incial de 109 U/L que se elevó hasta 7.689 U/L a las 12 horas) y una imposibilidad para la flexión dorsal pasiva de ambos tobillos. El paciente no sentía dolor en el resto de los compartimentos.
Resultados Ante la sospecha de un síndrome compartimental anterolateral, se realizó fasciotomía bilateral urgente, y se observó una disminución de la perfusión muscular y mejoría de la misma tras apertura de la fascia. Se realizaron curas periódicas cada 48 horas, y se observó viabilidad del tejido hasta su cierre definitivo a los 4 días. A las 2 semanas, el paciente presentó fatiga ligera para la deambulación sin ayuda, con tumefacción en el compartimento lateral de ambas piernas. A los 10 meses de evolución, el paciente caminaba sin ayuda y con función muscular completa.
Conclusiones El conocimiento de la asociación del síndrome compartimental y la cirugía laparoscópica prolongada es esencial para un diagnóstico precoz y un tratamiento quirúrgico inmediato, para evitar graves secuelas. Los buenos resultados de nuestro paciente se deben a la rápida actuación, ya que normalmente se suele demorar. Para evitar su aparición o disminuir su incidencia, la posición de litotomía debería limitarse a aquellos momentos de la cirugía en los que sea imprescindible, modificando la posición de las piernas cada dos horas en caso de cirugías prolongadas, para prevenir dicha complicación.
Information Security Awareness and Behaviors of Health Care Professionals at Public Health Care Facilities
Authors: Alhuwail Dari , Al-Jafar Eiman , Abdulsalam Yousef , AlDuaij Shaikha 
Published: September 2021

Abstract
Objectives This study investigated information security behaviors of professionals working in the public health sector to guide policymakers toward focusing their investments in infrastructure and training on the most vulnerable segments. We sought to answer the following questions: (1) Are certain professional demographics more vulnerable to cybersecurity threats? (2) Do professionals in different institution types (i.e., hospitals vs. primary care clinics) exhibit different cybersecurity behaviors? (3) Can Internet usage behaviors by professionals be indicative of their cybersecurity awareness and the risk they introduce?
Methods A cross-sectional, anonymous, paper-based survey was distributed among professionals working in public health care organizations in Kuwait. Data were collected about each professional's role, experience, work environment, cybersecurity practices, and understanding to calculate a cybersecurity score which indicates their level of compliance to good cybersecurity practices. We also asked about respondents' internet usage and used K-means cluster analysis to segment respondents into three groups based on their internet activities at work. Ordinary least squares regression assessed the association between the collected independent variables in question on the overall cybersecurity behavior.
Results A total of 453/700 (64%) were responded to the survey. The results indicated that professionals with more work experience demonstrated higher compliance with good cybersecurity practices. Interestingly, nurses demonstrate higher cybersecurity aptitude relative to physicians. Professionals that were less inclined to use the internet for personal use during their work demonstrated higher cybersecurity aptitude.
Conclusion Our findings provide some guidance regarding how to target health care professional training to mitigate cybersecurity risks. There is a need for ensuring that physicians receive adequate cybersecurity training, despite the opportunity costs and other issues competing for their attention. Additionally, classifying professionals based on their internet browsing patterns may identify individuals vulnerable to cybersecurity incidents better than more discrete indicators such as age or gender.
Protrusio acetabular en artroplastía total de cadera: diez tácticas para un buen resultado quirúrgico
Authors: Bengoa Francisco , López Alberto , Dabed David , Rojas Nicolas , Diaz-Ledezma Claudio 
Published: September 2021

ResumenLos pacientes candidatos a artroplastía total de cadera con protrusio acetabular asociada generan distintos desafíos en los equipos quirúrgicos. Múltiples estrategias han sido utilizadas a lo largo de los años para optimizar los resultados. Mediante una revisión de la evidencia actualizada disponible, proponemos diez tácticas a realizar en el manejo de estos pacientes que pueden mejorar y hacer predecible el tratamiento de un paciente con protrusio acetabular al que se le realiza una artroplastía total de cadera.
Nivel de Evidencia V.
Reconstrucción del ligamento patelofemoral medial mediante suturas transóseas patelares: estudio transversal de 34 pacientes
Authors: Pakuts Andrés , Martin Luis Fernando , Riquoir Christophe , Schadenberg Alex 
Published: September 2021

Resumen
Introducción La inestabilidad rotuliana recurrente puede conducir a malos resultados funcionales y daño articular progresivo a largo plazo, y con frecuencia se aborda mediante la reconstrucción del ligamento patelofemoral medial (RLPFM), con múltiples técnicas que suelen diferir en el método de fijación de la rótula.
Objetivo Evaluar los resultados de RLPFM utilizando una técnica novedosa de fijación rotuliana mediante suturas transóseas. El objetivo principal es determinar las tasas de reluxación y los resultados funcionales incluyendo retorno deportivo. Los objetivos secundarios incluyen la evaluación de las complicaciones y de otras variables relacionadas, y la comparación entre los resultados de RLPFM aislada y asociada a una osteotomía de la tuberosidad tibial.
Métodos Estudio transversal de 34 pacientes sometidos a RLPFM desde 2013 hasta 2019 con un seguimiento mínimo de 12 meses. La reconstrucción fue realizada por el mismo primer cirujano con autoinjerto de gracilis de doble banda en todos los casos. La fijación del injerto en la cara medial de la rótula se realizó utilizando dos puntos de fijación transóseos independientes con suturas de alta resistencia, y la fijación femoral anatómica, con un tornillo interferencial mediante referencias anatómicas y radiológicas. Hubo 27 pacientes con RLPFM aislada, y 7 con osteotomía de la tuberosidad tibial asociada.
Resultados La edad media fue de 22,8 años (desviación estándar [DE]: 9,1). El 50% eran hombres. El seguimiento desde la cirugía hasta el cuestionario fue de 30,4 meses (rango: 12 a 72 meses). La puntuación media de Kujala en el seguimiento fue de 89,4 (DE: 12,8; mediana: 93,5; rango: 51 a 100). No hubo casos de reluxación. No se identificaron otras complicaciones durante el seguimiento. El 81% de los pacientes regresó a los deportes, y el 47% regresó a su nivel previo de participación. No se encontraron diferencias significativas al comparar RLPFM aislada con grupos de osteotomía asociada.
Conclusión La RLPFM mediante sutura transósea para fijación en la rótula mostró que la estabilidad rotuliana fue restaurada en el corto y mediano plazo. Esta técnica es segura, tiene excelentes resultados funcionales, y evita posibles complicaciones de los túneles rotulianos o morbilidad asociada al uso de implantes.