bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forkhead transcription factor FKH-8 is a master regulator of primary cilia in C. elegans
Authors: Brocal-Ruiz Rebeca , Esteve-Serrano Ainara , Mora-Martinez Carlos , Swoboda Peter , Tena Juan , Flames Nuria 
Published: September 2021

SUMMARYCilia, either motile or non-motile (a.k.a primary or sensory), are complex evolutionary conserved eukaryotic structures composed of hundreds of proteins required for their assembly, structure and function that are collectively known as the ciliome. Ciliome mutations underlie a group of pleiotropic genetic diseases known as ciliopathies. Proper cilium function requires the tight coregulation of ciliome gene transcription, which is only fragmentarily understood. RFX transcription factors (TF) have an evolutionarily conserved role in the direct activation of ciliome genes both in motile and non-motile cilia cell types. In vertebrates, FoxJ1 and FoxN4 Forkhead (FKH) TFs work with RFX in the direct activation of ciliome genes, exclusively in motile cilia cell-types. No additional TFs have been described to act together with RFX in primary cilia cell-types in any organism. Here we describe FKH-8, a FKH TF, as master regulator of the primary ciliome in Caenorhabditis elegans. fkh-8 is expressed in all ciliated neurons in C. elegans, binds the regulatory regions of ciliome genes, regulates ciliome gene expression, cilium morphology and a wide range of behaviours mediated by sensory cilia. Importantly, we find FKH-8 function can be replaced by mouse FOXJ1 and FOXN4 but not by members of other mouse FKH subfamilies. In conclusion, our results show that RFX and FKH TF families act as master regulators of ciliogenesis also in sensory ciliated cell types and suggest that this regulatory logic could be an ancient trait predating functional cilia sub-specialization.
The snoGloBe interaction predictor reveals a broad spectrum of C/D snoRNA RNA targets
Authors: Deschamps-Francoeur Gabrielle , Couture Sonia , Abou-Elela Sherif , Scott Michelle S. 
Published: September 2021

ABSTRACTBox C/D small nucleolar RNAs (snoRNAs) are a conserved class of RNA known for their role in guiding ribosomal RNA 2’-O-ribose methylation. Recently, C/D snoRNAs were also implicated in regulating the expression of non-ribosomal genes through different modes of binding. Large scale RNA-RNA interaction datasets detect many snoRNAs binding messenger RNA, but are limited by specific experimental conditions. To enable a more comprehensive study of C/D snoRNA interactions, we created snoGloBe, a human C/D snoRNA interaction predictor based on a gradient boosting classifier. SnoGloBe considers the target type, position and sequence of the interactions, enabling it to outperform existing predictors. Interestingly, for specific snoRNAs, snoGloBe identifies strong enrichment of interactions near gene expression regulatory elements including splice sites. Abundance and splicing of predicted targets were altered upon the knockdown of their associated snoRNA. Strikingly, the predicted snoRNA interactions often overlap with the binding sites of functionally related RNA binding proteins, reinforcing their role in gene expression regulation. SnoGloBe is also an excellent tool for discovering viral RNA targets, as shown by its capacity to identify snoRNAs targeting the heavily methylated SARS-CoV-2 RNA. Overall, snoGloBe is capable of identifying experimentally validated binding sites and predicting novel sites with shared regulatory function.
Theta oscillations in anterior cingulate cortex and orbitofrontal cortex differentially modulate accuracy and speed in flexible reward learning
Authors: Ye Tony , Romero-Sosa Juan Luis , Rickard Anne , Aguirre Claudia G. , Wikenheiser Andrew M. , Blair Hugh T. , Izquierdo Alicia 
Published: September 2021

ABSTRACTFlexible reward learning relies on frontal cortex, with substantial evidence indicating that anterior cingulate cortex (ACC) and orbitofrontal cortex (OFC) subregions play important roles. Recent studies in both rat and macaque suggest theta oscillations (5-10 Hz) may be a spectral signature that coordinates this learning. However, network-level interactions between ACC and OFC in flexible learning remain unclear. We investigated the learning of stimulus-reward associations using a combination of simultaneousin-vivoelectrophysiology in dorsal ACC and ventral OFC, partnered with bilateral inhibitory DREADDs in ACC. In freely-behaving male and female rats and using a within-subject design, we examined accuracy and speed of response across distinct and precisely-defined trial epochs during initial visual discrimination learning and subsequent reversal of stimulus-reward contingencies. Following ACC inhibition there was a propensity for random responding in early reversal learning, with correct vs. incorrect trials distinguished only from OFC, not ACC, theta power differences in the reversal phase. ACC inhibition also hastened incorrect choices during reversal. This same pattern of change in accuracy and speed was not observed in viral control animals. Thus, characteristics of impaired reversal learning following ACC inhibition are poor deliberation and weak theta signaling of accuracy in this region. The present results also point to OFC theta oscillations as a prominent feature of reversal learning, unperturbed by ACC inhibition.
Endothelial-specific Gata3 expression is required for haematopoietic stem cell generation
Authors: Zaidan Nada , Diamanti Evangelia , Nitsche Leslie , Fidanza Antonella , Wilson Nicola K. , Forrester Lesley M. , Göttgens Berthold , Ottersbach Katrin 
Published: September 2021

AbstractTo generate sufficient numbers of transplantable haematopoietic stem cells (HSCs) in vitro, a detailed understanding of how this process takes place in vivo is essential. The endothelial-to-haematopoietic transition (EHT), which culminates in the production of the first HSCs, is a highly complex process during which key regulators are switched on and off at precise moments and which is embedded into a myriad of microenvironmental signals from surrounding cells and tissues. We have previously demonstrated an HSC-supportive function for Gata3 within the sympathetic nervous system and the sub-aortic mesenchyme, but show here that it also plays a cell-intrinsic role during the EHT. It is expressed in haemogenic endothelial cells and early HSC precursors, where its expression correlates with a more quiescent state. Importantly, endothelial-specific deletion of Gata3 shows that it is functionally required for these cells to mature into HSCs, placing Gata3 at the core of the EHT regulatory network.
Ovicidal Activity of 2-Hydroxy-4-Methoxybenzaldehyde, Derivatives and Structural Analogues on Anopheles gambiae eggs
Authors: Andati R. E , Omolo M. O. , Ndiege I.O. 
Published: September 2021

AbstractBackgroundEffective remedies for disrupting Anopheles gambiae metamorphosis at the egg stage are crucial in suppression of the malaria vector populations that result in the reduction of disease burden. 2-Hydroxy-4-methoxybenzaldehyde (the major component of Mondia whytei roots), its derivatives, structural analogues and their blends were evaluated against the eggs of An. gambiae in the search for ovicidal compounds with potential use in mosquito control programs.MethodsMature roots were harvested from Mondia whytei plants grown in the Center for African Medicinal & Nutritional Flora and Fauna (CAMNFF) herbal medicinal garden and cleaned with distilled water. 2-Hydroxy-4-methoxybenzaldehyde (1) was isolated by steam distillation of the chopped roots. The selected derivatives and/or analogues were prepared using established chemical procedures and their structures confirmed by NMR spectroscopy and ESI-MS. Ovicidal activity of the pure compounds, derivatives, structural analogues and/or formulated blends was tested at 1, 10, 25 and 50 ppm on An. gambiae eggs..ResultsEleven mono-substituted (3-7), di-substituted (8-10), tri-substituted (1-2) aromatic compounds were assayed for ovicidal activity against Anopheles gambiae eggs singly or as blends. Benzaldehyde (4) and 4-methoxybenzaldehyde (9) were further converted into 2-hydroxy-1, 2-diphenylethanone (11), 1, 5-diphenylpenta-1, 4-diene-3-one (12) and 1, 5-bis (4-methoxyphenyl) penta-1, 4-diene-3-one (13) and evaluated for ovicidal activity individually or as blends. Of the thirteen compounds evaluated individually, 2-hydroxy-4-methoxybenzaldehyde (1) exhibited the highest ovicidal activity at LC50 0.7075 ppm while anisole had the lowest activity at LC50 40.342 ppm. The derivatives exhibited moderate activity: 2-hydroxy-1, 2-diphenylethanone (LC50 10.599 ppm), 1, 5-diphenylpenta-1, 4-diene-3-one (LC50 9.019 ppm) and 1, 5-bis (4-methoxyphenyl) penta-1, 4-diene-3-one (LC50 15.642 ppm). The blends exhibited intriguingly high ovicidal efficacy with the mixture of benzaldehyde and phenol showing the highest (LC50 0.332 ppm) while phenol and anisole exhibited the lowest activity (LC50 9.9909 ppm).ConclusionFrom the activity of the blends, it is evident that anisole is antagonistic to the efficacy of phenol and benzaldehyde. It is also apparent that aldehyde and hydroxyl groups, when directly attached to the phenyl ring, provide the critical structural characteristics that contribute to the ovicidal activity of the aromatic compounds.
Bi-slant submanifolds of an S manifold
Authors: Akgün Aykut , Gülbahar Mehmet 
Published: September 2021

PurposeBi-slant submanifolds of S-manifolds are introduced, and some examples of these submanifolds are presented.Design/methodology/approachSome properties of Di-geodesic and Di-umbilical bi-slant submanifolds are examined.FindingsThe Riemannian curvature invariants of these submanifolds are computed, and some results are discussed with the help of these invariants.Originality/valueThe topic is original, and the manuscript has not been submitted to any other journal.
The effect of consumer group breadth and depth on movie sales: the mediating effect of eWOM-to-viewing ratio
Authors: Lee Jungwon , Lee Yunhye , Park Cheol 
Published: September 2021

PurposeThe purpose of this study is to analyze the effect on movie performance of the breadth and depth of consumer groups targeted by movies and to analyze the ways in which electronic word-of-mouth (eWOM) mediates these relationships.Design/methodology/approachFor empirical analysis, 45 days of sales and eWOM data for 63 movies released in Korea in 2017 were collected, and a panel regression analysis was conducted on a total of 2,835 data items. In addition, the analysis was rigorously verified through three robustness tests.FindingsThe breadth and depth of consumer groups targeted by movies have a non-linear relationship with sales, and this relationship is mediated by the eWOM performance of social media websites. In addition, eWOM performance has a non-linear relationship with sales, and these effects differ depending on the type of eWOM platform involved.Originality/valueThe effects of the breadth and depth of the consumer groups targeted by movies on eWOM performance and movie performance have not been sufficiently investigated. This paper expands on previous studies that reported a linear relationship between eWOM and sales by finding that the effects of consumer group breadth and depth on sales are not linear in terms of the mediation of eWOM performance. In addition, a new research direction is suggested by conceptualizing consumer group breadth and depth using eWOM data, on which basis the new concept of eWOM-to-viewing ratio (eWOM ratio) is proposed.
Setting the conditions for open innovation in the food industry: unravelling the human dimension of open innovation
Authors: Palumbo Rocco , Manesh Mohammad Fakhar , Pellegrini Massimiliano Matteo , Flamini Giulia 
Published: September 2021

PurposeThe human dimension of open innovation is paramount for organisational excellence. However, there is scant evidence of the implications of human resource management practices on employees' orientation towards open innovation. The article shows how such practices facilitate the development of an open innovation climate among food companies.Design/methodology/approachAn empirical study was designed to obtain insights into the approach to open innovation of a large sample of food companies (n = 2,458). Secondary data were collected from the sixth European Working Condition Survey. A parallel mediation analysis allowed us to investigate the human resource management practices' implications on individual perceptions of an open innovation-oriented organisational climate through the mediating effect of employees' involvement and engagement.FindingsHuman resource management practices have an impact on employees' skills, motivation and interpersonal relationships, but they do not have direct implications on the employees' perception of an open innovation-oriented organizational climate. As they solicit employees' involvement and engagement, human resource management practices indirectly nurture a favourable perception of an open innovation-oriented organisational climate.Practical implicationsTailored human resource management practices should be crafted to increase employees' capabilities and motivation and, therefore, to sustain open innovation in the food sector. Human resource management practices foster employees' involvement and engagement, which pave the way for a greater proclivity to open innovation at the individual and collective levels.Originality/valueThe article discusses the implications of human resource management practices on the perception of an organisational climate conducive to open innovation, envisioning aspects to focus on and avenues for future research.
Impact of women authentic leadership on their own mental wellbeing through ego depletion: moderating role of leader's sense of belongingness
Authors: Khan Naseer Abbas , Hui Zhang , Khan Ali Nawaz , Soomro Mohsin Ali 
Published: September 2021

PurposeLeadership research is of interest to academics and practitioners in the construction industry. Based on the ego-depletion theory and authentic leadership theory, the current study aims to investigate the impact of women authentic leadership on leaders' emotional exhaustion and job engagement in the construction industry.Design/methodology/approachThe data were gathered in two waves using a time lag approach. The responses of 276 women leaders-follower dyads from construction firms in China's eastern provinces were analyzed using a moderated mediation model.FindingsMost of the proposed hypotheses were supported by the findings of this study, which showed that authentic leadership can reduce emotional exhaustion in women leaders and increase work engagement through ego depletion. Furthermore, the leader's sense of belonging, according to this study, moderates the mediating effect of ego depletion.Research limitations/implicationsThis study can help managers, policymakers and human resource professionals think about authentic leadership and its impact on women leaders. Furthermore, ego depletion has an impact on the psychological well-being of authentic women leaders. The sense of belongingness of a leader is critical in buffering the negative effects of ego depletion for women in authentic leadership. Thus, women leaders in construction sector should be encouraged to express a sense of belonging to their followers, since this will improve their work engagement and lessen their emotional exhaustion.Originality/valueThis study is unique in that it examines the authentic behavior of women leaders in the Chinese construction sector, which is a challenging profession for women to work in as site managers. This study contributes to the literature on women in leadership by demonstrating how authentic leadership behavior influences the wellbeing and engagement of leaders. In addition, the study indicated that the effect of the mediator (ego depletion) and moderator (leader sense of belongingness) on the relationship between women's authentic leadership and the leader's own psychological wellbeing and job engagement was significant.
Soft system methodology for maintenance and treatment budgeting for public-rented flats
Authors: Arumsari Putri , Sulistio Hendrik 
Published: September 2021

Purpose
Public-rented flats in Jakarta Province operated by the Management Unit of Public-Rented Flats (MUPRF) experienced budget cuts for the maintenance and treatment activities during the COVID-19 pandemic that hit Indonesia in the early 2020. Currently, the budgeting scheme of the MUPRF uses the local government’s budget in determining the expenditures of public-rented flat. This papers aims to propose an alternative budgeting scheme for the MUPRF.

Design/methodology/approach
Soft system methodology (SSM) was adopted to understand the public-rented flats as a whole system, so an alternative budgeting scheme for the MUPRF can be identified and developed. Interviews with an employee of the Department of Community Housing and Settlement of Daerah Khusus Ibukota Jakarta Province were conducted. A rich picture, customer, action, transformation, worldview, owner and environment analyses, conceptual model and a proposed model were developed during the process.

Findings
Based on the SSM, it is found that becoming a local public service agency, the MUPRF can be more independent and flexible in managing their budget. The income generated by the public-rented flats can be used directly for their expenditure.

Research limitations/implications
Through the SSM, only a conceptual model is developed, which has not yet been implemented in practice. Future studies need to be carried out to evaluate the feasibility of the conceptual model.

Originality/value
This research analyses the public-rented flat as a whole system through SSM to identify factors and parties that are involved in the daily activities in public-rented flats to propose a suitable alternative for its budgeting scheme.
Physical–numerical parameters in laminar simulations of natural convection on three-dimensional square plates
Authors: Verdério Júnior Sílvio Aparecido , Scalon Vicente Luiz , Oliveira Santiago del Rio 
Published: September 2021

Purpose
The purpose of this study is to analyze the influence of the main physical–numerical parameters in the computational evaluation of natural convection heat transfer rates in isothermal flat square plates in the laminar regime. Moreover by experimentally validate the results of the numerical models and define the best parameter settings for the problem situation studied.

Design/methodology/approach
The present work is an extension of the study by Verderio Junior et al. (2021), differing in the modeling, results analysis and conclusions for the laminar flow regime with

Rade=1×105. The analysis of the influence and precision of the physical–numerical parameters: boundary conditions, degree of mesh refinement, refinement layers and κ – ω SST and κ – ε turbulence models, occurred from the results from 48 numerical models, which were simulated using the OpenFOAM® software. Comparing the experimental mean Nusselt number with the numerical values obtained in the simulations and the analysis of the relative errors were used in the evaluation of the advantages, restrictions and selection of the most adequate parameters to the studied problem situation.

Findings
The numerical results of the simulations were validated, with excellent precision, from the experimental reference by Kitamura et al. (2015). The application of the κ – ω SST and κ – ε turbulence models and the boundary conditions (with and without wall functions) were also physically validated. The use of the κ – ω SST and κ – ε turbulence models, in terms of cost-benefit and precision, proved to be inefficient in the problem situation studied. Simulations without turbulence models proved to be the best option for the physical model for the studies developed. The use of refinement layers, especially in applications with wall functions and turbulence models, proved unfeasible.

Practical implications
Use of the physical–numerical parameters studied and validated, and application of the modeling and analysis methodology developed in projects and optimizations of natural convection thermal systems in a laminar flow regime. Just like, reduce costs and the dependence on the construction of experimental apparatus to obtain experimental results and in the numerical-experimental validation process.

Social implications
Exclusive use of free and open-source computational tools as an alternative to feasible research in the computational fluid dynamics area in conditions of budget constraints and lack of higher value-added infrastructure, with applicability in the academic and industrial areas.

Originality/value
The results and discussions presented are original and new for the applied study of laminar natural convection in isothermal flat plate, with analysis and validation of the main physical and numerical influence parameters.
Social entrepreneurship finance: the gaps in an innovative discipline
Authors: Parekh Nadiya , Attuel-Mendès Laurence 
Published: September 2021

PurposeSocial entrepreneurship is gaining increased attention from academia and practitioners worldwide. Owing to its financing challenges, academic pedagogies are seeking methods to strengthen the social financing dimension of this emerging discipline. This paper bridges the gap in social entrepreneurship education by portraying diverse perspectives on this topic from multiple actors in two cross-cultural contexts.Design/methodology/approachA qualitative case analysis was conducted to explore financing aspects of social entrepreneurship in France and the United States. The authors interviewed academicians and practitioners to learn about their current experiments and thoughts on integrating finance into the curriculum for social entrepreneurship.FindingsThe authors found multiple facets of the social entrepreneurship finance construct, focused not only on specific financial skills but also on a general approach to venture designs. Multidisciplinary knowledge is sought not just on the topic of finance but also in other disciplines that can broaden its scope of financing to a larger investor domain. While in France, this came out as a need for integrating the financial communication skills to personify the social value creation process; in the US, it was pointed out as the need for having a contractual knowledge to differentiate investment opportunities and comprehend their risks levels.Originality/valueBy bringing perspectives from multiple actors who have had experience in social entrepreneurship financing in regions with the fastest development, this paper is seminal in bridging the financing skill gaps that exist in social entrepreneurship discipline. The main theoretical contribution of this article concerns the skills, financial and otherwise that are useful in social finance.