bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic Effect of Saikosaponin a and Albiflorin Against Corticosterone-Induced Neuro-Injury in PC12&nbsp;Cells via Regulation of TCA&nbsp;Cycle, Purine Metabolism, and Glutamate Metabolism
Authors: Li Xiao , Hou Ruihong , Shi Hao , Qin Xuemei , Wu Yanfei , Wu Xingkang , Tian Junsheng , Gao Xiaoxia , Du Guanhua , Zhou Yuzhi 
Published: February 2021

Abstract
The combination of Radix Bupleuri - Radix Paeoniae alba is one of the most approbated herb pairs in traditional Chinese medicine (TCM) formula for curing depression. Saikosaponin A (SSA) and albiflorin (AF) are major bioactive compounds of Radix Bupleuri and Radix Paeoniae alba respectively, which possess antidepressant effects in pharmacological experiments. However, whether SSA and AF have synergistic neuroprotective effects and the synergistic mechanism is still unknown. The present study employed the corticosterone-induced PC12 cells neuro-injury model to assess the protective effect of SSA and AF, alone and in combination, and the synergistic effect was analyzed using three mathematical models. Meanwhile, the cell metabolomics was used to detect the effects on metabolite regulation of SSA and AF, alone and in combination. According to the results of cell metabolomics, the vital cell transduction pathway related to the crucial cell metabolic pathways were selected. Furthermore, the key metabolites, metabolic enzymes, and cellular markers were verified by ELISA and Western blotting. The results showed that the combination of SSA and AF has a synergistic neuroprotective effect. Besides, the combination could regulate more metabolites (16) than a single agent (12 & 10) and possessed a stronger adjustment effect on metabolites. Correspondingly, the combination regulated more metabolism pathways (7) than alone agents (3 & 5). Furthermore, the TCA cycle, purine metabolism, and glutamate metabolism were selected as crucial metabolism pathways. The results showed that the TCA cycle disorder was regulated by SSA and AF via improving mitochondrial function. The purine metabolism disorder was regulated by SSA via inhibition xanthine oxidase (XOD) activity and the glutamate metabolism disorder was regulated by AF via inhibition glutaminase (GLS) activity. Moreover, the oxidative stress induced by the purine metabolism disorder was attenuated by SSA via a reduction in the ROS level. Additionally, the inflammation induced by the oxidative stress was attenuated by the SSA and AF via inhibition of the NLRP3 protein expression. This study for the first time demonstrated the synergistic neuroprotective effect of SSA and AF, and the synergistic mechanism was involved in metabolic disorders regulation.
Identification of Differentially Expressed Genes and Signalling Pathways Related to Ovarian Endometriosis Based on Integrated Bioinformatic Analysis
Authors: Lin Kainan , Pan Zhenyan , He Renke , Wang Hanchu , Zhou Kai , Mu Liangshan 
Published: February 2021

Abstract
Purpose: Endometriosis is a common gynaecological disease; however, the specific mechanism and the key molecules involved in endometriosis have not been elucidated. This study aimed to identify key genes associated with poor prognosis and further uncover underlying mechanisms.Methods: Data regarding mRNA expression profiles used in this study were retrieved from the Gene Expression Omnibus (GEO) database, and a total of three mRNA expression profiles were included in subsequent analyses (GSE31515, GSE58178 and GSE120103). We divided all differentially expressed genes (DEGs) into up-regulated and down-regulated groups. Then, we conducted Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and protein-protein interaction (PPI) analysis using R software.Results: A total of 304 DEGs were identified between endometriosis tissues and normal endometrium tissues using integrated analysis, including 185 up-regulated genes and 119 down-regulated genes. GO analysis revealed that the up-regulated DEGs of endometriosis were closely associated with voltage-gated calcium channel activity, whereas the down-regulated DEGs were enriched in uterus development. KEGG pathway enrichment analysis indicated that the up-regulated DEGs were mainly involved in cytokine-cytokine receptor interaction, whereas down-regulated DEGs were enriched in protein processing in the endoplasmic reticulum. In addition, PPIs of these DEGs were visualized using the Cytoscape platform and the Search Tool for the Retrieval of Interacting Genes (STRING). PPI analysis identified 10 potential DEG-related protein targets, including CCND1, IL6, CCL2, COL1A2, PTGS2, VCAM1, COL3A1, ELN, SERPINE1, and HSP90B1. Conclusion: In conclusion, the present study reveals that voltage-gated calcium channel activity, uterus development, cytokine-cytokine receptor interaction and protein processing in the endoplasmic reticulum may be involved in the development of endometriosis. In addition, these identified DEGs may exhibit clinical significance for the diagnosis and treatment of endometriosis.
Weathering effects on engineering properties of Schist of Menderes Massif, West of Turkey
Authors: Geçkin Berna Şengöçmen , Turk Necdet , Moses Dyson 
Published: February 2021

Abstract
Schist is a metamorphic rock type that is widely exposed in the Western Anatolia. Schist of Menderes Massif is located in different locations and geological levels. The rock is mainly used as a filling and building material in the present and in foreseeable future. Usage of schist as filling materials in dams is related with their weathering degree which affects their geomechanical properties. To determinate the petrographic, chemical, index and engineering properties of the schist used as filler material in the dams/small dam crest, fresh and weathered samples of rock material were collected from quarries. The relationship between the weathering degree index and engineering properties were determined by simple regression analyses’. The results show that the studied parameters of physical and mechanical properties of the studied schist have a positive correction with weathering grade. Thus, increasing weathering grade affects the durability of the schist thereby compromising its applicability.
An antimicrobial&nbsp;Staphylococcus sciuri&nbsp;with broad temperature and salt spectrum isolated from the surface of the African social spider,&nbsp;Stegodyphus dumicola
Authors: Nazipi Seven , Vangkilde-Pedersen Sofie Gerdes , Busck Mette Marie , Lund Dorthe Kirstine , Marshall Ian P.G. , Bilde Trine , Lund Marie Braad , Schramm Andreas 
Published: February 2021

Abstract
Some social arthropods engage in mutualistic symbiosis with antimicrobial compound-producing microorganisms that provide protection against pathogens. Social spiders live in communal nests and contain specific endosymbionts with unknown function. Bacteria are also found on the spiders' surface, including prevalent staphylococci, which may have protective potential. Here we present the genomic and phenotypic characterization of strain i1, isolated from the surface of the social spider Stegodyphus dumicola. Phylogenomic analysis identified i1 as novel strain of Staphylococcus sciuri within subgroup 2 of three newly defined genomic subgroups. Further phenotypic investigations showed that S. sciuri i1 is an extremophile that can grow at a broad range of temperatures (4°C-45°C), high salt concentrations (up to 27%), and has antimicrobial activity against closely related species. We identified a lactococcin 972-like bacteriocin gene cluster, likely responsible for the antimicrobial activity, and found it conserved in two of the three subgroups of S. sciuri. These features indicate that S. sciuri i1, though not a specific symbiont, is well-adapted to survive on the surface of social spiders and may gain a competitive advantage by inhibiting closely related species.
COVID-19 remote consultation services and population in health inequity-concentrating territories: a scoping review protocol
Authors: Silva Angélica Baptista , Síndico Sérgio , Gomes Joyker Peçanha , Carneiro Ana Carolina , Fernandes Arthur Gustavo , Henrique Sávio Mourão , Marinho Thaysa Pereira , Fernandes Valcler Rangel 
Published: February 2021

Abstract
Objective: This scoping review aims to map initiatives of measuring the vulnerable populations' satisfaction with teleconsultation and remote consultation services offered by public and private healthcare providers in their respective territories during the COVID-19 pandemic.Introduction: New teleconsultation-based services were implemented during the pandemic with their monitoring and evaluation processes. It is necessary to know these assessments if services worsen the disparities between vulnerable and economically disadvantaged populations. Understanding whether the services meet the population's expectations concerning the care to contain the pandemic in their territory can support inclusive policies.Inclusion criteria: This review will include studies on evaluations of teleconsultation-based services offered to the socioeconomically disadvantaged population of a given territory focused on the surveillance, treatment, and prevention of COVID-19. The assessments should describe the process of determining the satisfaction of the population served.Methods: A comprehensive search will be performed in MEDLINE, EMBASE, Web of Science databases, and other documents in Scopus to identify gray literature. Documents published in English, Portuguese and Spanish will be included from December 2019. The strategy combines descriptors and keywords related to telehealth, Covid-19, patient satisfaction, and vulnerable populations. According to the inclusion criteria, two reviewers will screen titles and abstracts and select the articles for evaluation. The articles selected for full-text screening will be submitted to data extraction, using a tool developed by the third reviewer and approved by the other authors. The data will be presented in semantic networks and narrative summary, aligning with the reviews' objectives and scope.
Prevalence of Depression Symptoms and Associated Sociodemographic and Clinical Correlates Among Syrian Refugees in Lebanon
Authors: Naal Hady , Nabulsi Dana , Arnaout Nour El , Abdouni Lina , Dimassi Hani , Harb Ranime , Saleh Shadi 
Published: January 2021

Abstract
Background: Since the outbreak of the Syrian war in 2011, close to 6 million Syrian refugees have escaped to Syria’s neighbouring countries, including Lebanon. Evidence suggests rising levels of mental health disorders among Syrian refugee populations. Yet, to the best of our knowledge, large-scale studies addressing the mental health of adult Syrian refugees in Lebanon are lacking. Aims: We examined the prevalence of depression symptoms, which represent a common and debilitating mental health disorder among Syrian refugee populations in Lebanon, along with their sociodemographic and clinical correlates.Methods: A cross-sectional survey design was conducted as part of a collaborative project-“Sijilli”- led by the Global Health Institute at the American University of Beirut (Beirut, Lebanon) across 4 informal tented settlements for refugees (Beirut, Bekaa, North, South) in Lebanon among adult Syrian refugees (≥18), over a period extending from 2018 to 2020. The survey inquired about participants’ sociodemographic and clinical characteristics, and screened participants for symptoms of depression through sequential methodology using the Patient Health Questionnaire (PHQ-2 and PHQ-9). Results: A total of 3255 adult Syrian refugees were enrolled in the study. Of those refugees, only 51.6% (n=1678) screened positive on the PHQ-2 and were therefore eligible to complete the PHQ-9. The PHQ-9 analysis revealed high prevalence (25%) of moderate to severe depression in the total sample, suggestive of high probability for major depression disorder (MDD). Further analyses indicate that being ≥45 years of age (OR 1.70, 95% CI 1.22-2.36), a woman (OR 1.35, 95% CI 1.07-1.69), divorced/separated (OR 3.32, 95% CI 1.57-7.01), reporting a neurological (OR 1.77, 95% CI 1.20-2.61) or a mental health condition (OR 5.30, 95% CI 2.40-11.66) are major risk factors for MDD.Conclusion: Our study suggests that one in four Syrian refugees in Lebanon have probable MDD, and our findings have important public health and clinical implications on refugee health. There is a need to enhance screening efforts, to improve access and referral to mental health services, and to improve post-migration factors among Syrian refugees in Lebanon.
Dinámica de paquetes de ondas de huecos bajo acoplamiento espín-órbita tipo Rashba
Authors: Cuan R. , Diago-Cisneros L. 
Published: February 2021

Se estudia la dinámica de un paquete de ondas gaussiano de huecos pesados, en presencia de interacción espín-órbita tipo Rashba, para un sistema semiconductor cuasi-unidimensional. Se utiliza un esquema en diferencias finitas, basado en la aproximación de Cayley, y se extiende a la solución de la ecuación de Schrödinger dependiente del tiempo para huecos. Se muestra el fenómeno de la precesión del espín de los huecos, a través de una simulación numérica de la evolución temporal de las componentes del paquete. Es posible evaluar parámetros relevantes, e. g. dimensiones y tiempos de inversión de la polarización, en un dispositivo modelado como transistor de efecto campo de espín, que utilice en calidad de portadores a los huecos.
Simultaneous Determination of Amlodipine and Irbesartan in their
Pharmaceutical Formulations by Square-Wave Voltammetry
Authors: Palabıyık İsmail Murat , Dogan Aysegul , Süslü İncilay 
Published: January 2021

Background:
Hypertension is one of the most important health problems in the world and irbesartan and amlodipine are used in combination in various dosages for the treatment of high blood pressure.

Objective:
The aim of this study is to develop a fast, easy, sensitive, accurate, and precise square-wave voltammetry method for simultaneous determination of irbesartan and amlodipine besylate from pharmaceutical formulations at a hanging mercury drop electrode.

Methods:
In the applied method, since both active substances gave a peak at different potentials, no interference occurred between them. In optimization studies Britton-Robinson buffer of pH 8.0 was chosen, in which the most appropriate peak shape and maximum peak current were observed. At the same time, as a result of instrumental parameter optimization to obtain reproducible results, 6 mV for scan increment, 30 mV for pulse amplitude, and 50 Hz for frequency were found suitable.

Results:
As a result of the calibration studies of the optimized method, linear working ranges were determined as 1.00-13.08 µg mL-1 for irbesartan and 5.83-16.51 µg mL-1 for amlodipine besylate. Limit of detection and limit of quantitation values were respectively calculated as 0.63 and 1.00 µg mL-1 for irbesartan and 0.50 and 1.98 µg mL-1 for amlodipine besylate. The results of precision values (RSD) ranged from 0.67% to 2.31% for irbesartan and 0.65% to 1.49% for amlodipine besylate. Accuracy values were calculated as -0.15% to 1.63% for irbesartan and -0.07% to 3.78% for amlodipine besylate. The results obtained from the recovery studies ranged from 101.05% to 102.78% and from 98.88% to 102.20% for amlodipine besylate and irbesartan, respectively.

Conclusion:
After the validation studies of the developed method were carried out, it was successfully applied to pharmaceutical formulations containing these active substances.
A Review on Pancreatic Lipase Inhibitors from Natural Sources: A Potential Target for Obesity
Authors: T Bency Baby , R Murali , TNK Suriyaprakash , N Srinivasan 
Published: January 2021

Background:
Obesity is a worldwide lifestyle disorder and its incidence is growing at an
alarming rate. Pancreatic lipase (PL) plays a key role in lipid metabolism and hence is a potential target
for obesity treatment. There have been reports that many bioactive compounds from natural sources constitute
a vast pool of PL inhibitors.

Objective:
In search of natural pancreatic lipase inhibitors, the review summarizes the potential of natural
products for their pancreatic lipase inhibitory activity.

Methods:
: In this review, the data obtained from literature search of various electronic databases and journal
article publications have been reviewed.

Results:
The pancreatic lipase inhibitory activities of 61 biological sources reviewed in this paper are
backed by preclinical experimental evidence, either in vivo or in vitro.

Conclusion:
This article can be used as a ready-to-use reference for therapeutic lead molecules from
plants, marine and insect-derived natural products with PL inhibitory activity. The research is more focused
on the discovery of more ingenious pancreatic lipase inhibitors with lesser consequences.
Can Probiotics Supplementation Improve Glycemic and Renal Status in
Diabetic Nephropathy? A Systematic Review and Meta-Analysis of Clinical
Trials
Authors: Tarrahi Mohammad Javad , Namjoo Iman , Borzoo-Isfahani Mohammad , Ebdali Hadiseh , Moravejolahkami Amir Reza 
Published: January 2021

Background & Aims:
This meta-analysis was performed to quantify the effects of probiotics on renal and
glycemic biomarkers among patients with Diabetic Nephropathy (DN).

Methods:
Electronic databases were searched through May 10, 2020. All trials that investigated the effect of probiotics on
serum glycemic markers (Fasting Plasma Glucose [FPG], Hemoglobin A1C, Insulin, Homeostatic Model AssessmentInsulin Resistance [HOMA-IR], and Quantitative Insulin Sensitivity Check Index [QUICKI]), and renal status markers
(Creatinine [Cr], Blood Urea Nitrogen [BUN], and Glomerular Filtration Rate [GFR]) were included.

Results:
Seven trials that included 340 patients were identified for analysis. The results indicated that probiotics
significantly reduced FPG (WMD= -19.08 mg/dl; 95% CI= -32.16, -5.99; P=0.004), HOMA-IR (WMD= -1.88; 95% CI= -
3.63, -0.12; P=0.036), and Cr (WMD= -0.18 mg/dl; 95% CI= -0.26, -0.09; P<0.001) levels in DN patients; however, there
was no statistically significant change in Hemoglobin A1C, Insulin, QUICKI, BUN, and GFR.

Conclusion:
This meta-analysis supports the potential use of probiotics in the improvement of some glycemic and renal
biomarkers in patients with DN.
Evaluación agronómica y nutricional de ocho líneas de fríjol Caupí (Vigna unguiculata [L.] Walp.)
Authors: Cardona Villadiego Carlos Enrique , Araméndiz-Tatis Hermes , Espitia-Camacho Miguel Mariano , Cardona-Ayala Carlos Enrique 
Published: February 2021

Contextualización: el fríjol caupí es importante para la seguridad alimentaria de la región Caribe de Colombia, donde se cultivan genotipos criollos de bajo rendimiento por hectárea, susceptibles a factores bióticos y abióticos, y bajos contenidos de minerales. Vacío de conocimiento: se desconoce el comportamiento agronómico y la acumulación de minerales en el grano, de nuevos genotipos obtenidos por selección individual. Propósito del estudio: evaluar el comportamiento agronómico y la calidad nutricional de ocho líneas homocigotas avanzadas de fríjol caupí del programa de mejoramiento de la Universidad de Córdoba, Colombia. Metodología: el experimento se estableció entre abril a julio de 2014, bajo un diseño en bloques completos aleatorizados con nueve tratamientos incluido el testigo Criollo-Córdoba, con cuatro repeticiones. Se evaluaron las características longitud de la vaina, longitud del grano, ancho del grano, peso de la vaina, número de semilla por vaina, peso de cien semillas, rendimiento de grano por hectárea y contenidos de hierro, zinc, fósforo y proteína. Resultados y conclusiones: los genotipos presentaron diferencias significativas en número de semillas por vaina, longitud del grano, ancho de grano, rendimiento de grano por hectárea, contenido de hierro, fósforo y proteína en el grano. Las líneas L-042 y L-047 fueron las de mejor comportamiento agronómico y calidad nutricional. El análisis de componentes principales y el biplot permitieron identificar a los genotipos L-014 y L-047 asociados a longitud del grano, longitud de vaina y peso de vaina, mientras que L-006, L-042 y CR-CO, están asociados a ancho de grano, peso de cien semillas y rendimiento de grano por hectárea, lo cual es importante para los programas de mejoramiento genético.
Métodos de escarificación química y sus efectos en la germinación de semillas de Ochroma pyramidale Cav. ex Lam. Urb
Authors: Rios Geovo Virleydys , Córdoba-Tovar Leonomir , Ramírez Mosquera Pedro Luis , Copete Arroyo José Harry , Ramos Barón Pablo Andrés 
Published: February 2021

Introducción: la Ochroma pyramidale es una especie con alto potencial restaurador y se adapta fácilmente en áreas perturbadas; sin embargo, la germinación de la semilla se ve afectada por ser ortodoxa y experimentar latencia.
Vacío de conocimiento: variación de la capacidad germinativa entre distintos tratamientos y condiciones ambientales.
Propósito del estudio: evaluar el porcentaje de germinación de semillas de O. pyramidale por medio de diferentes métodos de escarificación química.
Metodología: se aplicó un diseño experimental de tres bloques, tres repeticiones y 15 unidades de ensayos. Los tratamientos fueron: T1 (agua caliente a 80°C), T2 (NaCIO al 0,5%), T3 (NaCIO al 1,0%), T4 (NaCIO al 2,0%), T5 (HCl al 1%).
Resultados y conclusiones: la germinación de semillas de O. pyramidale en todos los tratamientos inició al cuarto día después de la siembra con algunas diferencias de semillas germinadas entre tratamiento con respecto al primer día de germinación con algunas variaciones entre tratamientos. El mayor porcentaje de germinación ocurrió en el tratamiento 1 (T1) con 88 semillas (59%), seguido del tratamiento 2 (T2) con 65 (43%). El tratamiento 1 (agua 80°C) tuvo mayor influencia en la germinación de semillas de O. pyramidale. Por lo tanto, se concluyen que los tratamientos por ebullición no solo contribuyen en el incremento de la germinación, sino que además las semillas logran mayor velocidad germinativa.
Evaluación toxicológica del agua y los sedimentos en el embalse La Fe, Colombia
Authors: Amaringo Villa Fredy Alberto , Molina Perez Francisco Jose 
Published: February 2021

Contextualización del tema: la calidad del agua de los embalses destinados al tratamiento y suministro de agua potable se debe monitorear mediante análisis de toxicidad del agua y sedimentos, ya que los sedimentos pueden actuar como fuente de contaminantes orgánicos, de tal forma que durante procesos de mezcla y transporte estos contaminantes pueden quedar disponibles en los ecosistemas acuáticos. Vacío de investigación: debido a que estos contaminantes esta en el orden de trazas, se hace necesaria la utilización de metodos de muestreo pasivo que permitan la acumulación de compuestos hidrofóbicos en dispositivos de membranas semipermeables (SPMDs) para la posterior evaluación. Propósito del estudio: en este estudio, se evaluó la toxicidad de extractos dializados de muestreo pasivo tipo SPMD y de sedimentos, mediante ensayos Microtox®, usando la bacteria Vibrio fischeri. Metodología: los 24 extractos analizados se obtuvieron a partir de dispositivos de membranas semipermeables (SPMDs) previamente desplegadas durante 21 días en seis estaciones en el cuerpo de agua del embalse La Fe, Antioquia, durante cuatro campañas de muestreo. Los extractos se sometieron a ensayo mediante el Microtox Basic Test. La evaluación del potencial de toxicidad del sedimento del embalse se analizó de igual forma en 12 muestras, mediante ensayos de toxicidad aguda con Microtox®, aplicando el protocolo Solid Phase Test (SPT). Asimismo, se determinaron los parametros fisicoquímicos del agua pH, temperatura, conductividad eléctrica, oxígeno disuelto y turbidez. Resultados: los resultados de los ensayos mostraron que los extractos de SPMD recolectados en el cuerpo de agua no presentaron toxicidad, mientras que los elutriados de sedimentos presentaron una toxicidad moderada o baja.