bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor-driven invasion profiles in diffuse intrinsic pontine glioma
Authors: Karki Anju , Berlow Noah E , Kim Jin-Ah , Hulleman Esther , Liu Qianqian , Michalek Joel E , Keller Charles 
Published: March 2021

Abstract

Background
Diffuse intrinsic pontine glioma (DIPG) is a devastating pediatric cancer with unmet clinical need. DIPG is invasive in nature, where tumor cells interweave into the fiber nerve tracts of the pons making the tumor unresectable. Accordingly, novel approaches in combating the disease are of utmost importance and receptor-driven cell invasion in the context of DIPG is under-researched area. Here, we investigated the impact on cell invasion mediated by PLEXINB1, PLEXINB2, platelet growth factor receptor (PDGFR)α, PDGFRβ, epithelial growth factor receptor (EGFR), activin receptor 1 (ACVR1), chemokine receptor 4 (CXCR4), and NOTCH1.

Methods
We used previously published RNA-sequencing data to measure gene expression of selected receptors in DIPG tumor tissue versus matched normal tissue controls (n = 18). We assessed protein expression of the corresponding genes using DIPG cell culture models. Then, we performed cell viability and cell invasion assays of DIPG cells stimulated with chemoattractants/ligands.

Results
RNA-sequencing data showed increased gene expression of receptor genes such as PLEXINB2, PDGFRα, EGFR, ACVR1, CXCR4, and NOTCH1 in DIPG tumors compared to the control tissues. Representative DIPG cell lines demonstrated correspondingly increased protein expression levels of these genes. Cell viability assays showed minimal effects of growth factors/chemokines on tumor cell growth in most instances. Recombinant SEMA4C, SEM4D, PDGF-AA, PDGF-BB, ACVA, CXCL12, and DLL4 ligand stimulation altered invasion in DIPG cells.

Conclusions
We show that no single growth factor-ligand pair universally induces DIPG cell invasion. However, our results reveal a potential to create a composite of cytokines or anti-cytokines to modulate DIPG cell invasion.
Gender Differences in Methamphetamine Use Initiation and Trajectory of Use Among People Who Use Methamphetamine in a Mexico-US Border City
Authors: Loza Oralia , Guevara Priscilla , Hernandez Amir 
Published: March 2021

Introduction:
Methamphetamine (meth) is a stimulant increasing in use and its prevalence has not yet been determined on the Mexico-US border. Few studies highlight gender differences in meth use, trajectory, and initiation by gender. Ciudad Juárez, Mexico, across the border from El Paso, TX, has an established stimulant using population and lies on drug trafficking route. This study assessed gender differences in drug and meth use patterns among people meth people use meth.

Materials and Methods:
This cross-sectional study included 150 people with recent meth use, age 21 years or older, and living in Ciudad Juárez. Measures collected included sociodemographic characteristics, cross-border mobility, drug, and meth use characteristics. Statistically (P<0.05) and marginally (P<0.10) significant gender differences were determined using appropriate bivariate tests.

Results:
The sample included 45 cisgender women, 5 transgender women, and 100 cisgender men with a mean age of 30.9 years. Men had significantly higher rates of snorting meth by nose in powder or rock forms, while trans/women more frequently using meth in pill form. Men had higher rates of crossing the border for work, with meth, and to obtain meth. Trans/women reported higher rates of acquiring meth from their main sexual partner and initiating meth use in Mexico.

Discussion:
There are gender differences in patterns of meth use and initiation.

Conclusions:
A sample of people who use meth in the general population can be achieved. These findings point to a need for evidence-based meth use reduction treatment programs that are culturally appropriate and tailored for gender.
Acquisition and Interpretation of Digital Information in Forensic Assessments
Authors: Burton Barbara , Drogin Eric Y. 
Published: March 2021

Abstract
Despite the now ubiquitous presence of technology in everyday life, there is little written on the subject of personal digital data as it pertains to forensic mental health evaluations. Ethical concerns and limited consensus guidance on this issue have previously made it challenging to use this information in a responsible way. This Perspectives article elaborates on the different types of personal data that exist and the practical aspects of acquiring such information, including data location and legal considerations. Incorporation of personal digital information into the forensic assessment as a form of collateral information is discussed, along with the current state of the literature on technology use and its relationship to psychopathology and risk. Given the important role of technology in the life of the modern individual, deeper research into this topic will be necessary in the immediate future to establish practice standards for the safe and ethical use of digital information in both forensic and non-forensic mental health assessments.
New Approach to the Surveillance of Pediatric Infectious Diseases From Ambulatory Pediatricians in the Digital Era
Authors: Cohen Robert , Béchet Stéphane , Gelbert Nathalie , Frandji Bruno , Vie Le Sage François , Thiebault Georges , Kochert Fabienne , Cahn-Sellem Fabienne , Werner Andreas , Ouldali Naim , Levy Corinne 
Published: March 2021

Background:
Many ambulatory networks in several countries have established syndromic surveillance systems to detect outbreaks of different illnesses. Here, we describe a new Pediatric and Ambulatory Research in Infectious diseases network that combined automated data extraction from the computers of primary care pediatricians.

Methods:
Pediatricians who used the same software, AxiSanté 5-Infansoft for electronic medical records were specially trained in infectious diseases, encouraged to comply with French treatments’ recommendations, use of point-of-care tests and vaccination guidelines. Infectious disease diagnoses in children <16 years old in the records triggered automatic data extraction of complete records. A quality control process and external validation were developed.

Results:
From September 2017 to February 2020, 107 pediatricians enrolled 57,806 children (mean age 2.9 ± 2.6 years at diagnosis) with at least one infectious disease diagnosis among those followed by the network. Among the 118,193 diagnoses, the most frequent were acute otitis media (n = 44,924, 38.0%), tonsillopharyngitis (n = 13,334, 11.3%), gastroenteritis (n = 12,367, 10.5%), influenza (n = 11,062, 9.4%), bronchiolitis (n = 10,531, 8.9%), enteroviral infections (n = 8474, 7.2%) and chickenpox (n = 6857, 5.8%). A rapid diagnostic test was performed in 84.7% of cases of tonsillopharyngitis and was positive in 44%. The antibiotic recommendations from French guidelines were strictly followed: amoxicillin was the most prescribed antibiotic and less than 10% of presumed viral infections were treated.

Conclusions:
This “tailor-made” network set up with quality controls and external validation represents a new approach to the surveillance of pediatric infectious diseases in the digital era and could highly optimize pediatric practices.
Impact of Simultaneous Pancreas-kidney Transplantation on Cardiovascular Risk in Patients With Diabetes
Authors: Montagud-Marrahi Enrique , Molina-Andújar Alicia , Pané Adriana , Ruiz Sabina , Amor Antonio J. , Esmatjes Enric , Ferrer Joana , Banon-Maneus Elisenda , Hermida Evelyn , Musquera Mireia , Fondevila Constantino , Diekmann Fritz , Ventura-Aguiar Pedro 
Published: March 2021

Background.
Cardiovascular disease is the major cause of death in patients with type 1 diabetes. Of the available risk predictors for this population, the Steno Type 1 Risk Engine (STENO T1) is the only one that includes kidney function as a risk factor, which is a well-described independent risk factor for cardiovascular disease.

Methods.
We explore how simultaneous pancreas-kidney transplantation (SPKT) modifies the predicted cardiovascular risk by the STENO T1 through a retrospective study including recipients of a first SPKT between 2000 and 2016.

Results.
Two hundred sixty-eight SPKT recipients with a mean age of 40 y old and a median follow-up of 10 y were included. Before transplantation, the expected incidence of cardiovascular events (CVEs) at 5 and 10 y according to STENO T1 would have been 31% and 50%, respectively, contrasting with an actual incidence of 9.3% and 16% for the same timepoints, respectively (P < 0.05). These differences were attenuated when STENO T1 was recalculated assuming 12th-mo glomerular filtration rate (at 5 and 10 y predicted CVE incidence was 10.5% and 19.4%, respectively). Early pancreas graft failure (hazard ratio [HR] 3.00, 95% confidence interval [CI], 1.14-7.88; P = 0.02) was an independent risk factor for post-SPKT CVE, alongside kidney graft failure (HR 2.90, 95% CI, 1.53-5.48; P = 0.001), and diabetes duration (HR 1.04, 95% CI, 1.00-1.09, P = 0.04).

Conclusions.
SPKT decreases in more than two-thirds of the predicted cardiovascular risk by the STENO T1. A functioning pancreas graft further reduces CVE risk, independently of kidney graft function.
Effect of wall type, delayed mortality and mosquito age on the residual efficacy of a clothianidin-based indoor residual spray formulation (SumiShield™ 50WG) in southern Mozambique
Authors: Marti-Soler Helena , Maquina Mara , Opiyo Mercy , Alafo Celso , Sherrard-Smith Ellie , Malheia Arlindo , Cuamba Nelson , Sacoor Charfudin , Rabinovich Regina , Aide Pedro , Saúte Francisco , Paaijmans Krijn 
Published: March 2021

AbstractBackgroundIndoor residual spraying (IRS) is one of the main malaria vector control strategies in Mozambique alongside the distribution of insecticide treated nets. As part of the national insecticide resistance management strategy, Mozambique introduced SumiShield™ 50WG, a third generation IRS product, in 2018. Its residual efficacy was assessed in southern Mozambique during the 2018-2019 malaria season.MethodsTwo different wall surfaces, cement and mud-plastered surfaces, daily mosquito mortality up to 120 hours post-exposure, and older mosquitoes (13-26d old) were included in standard WHO (World Health Organization) cone bioassay tests. Lethal times (LT) 90, LT50 and LT10 were estimated using Bayesian models.ResultsMortality 24h post exposure was consistently below 80%, the current WHO threshold value for effective IRS, in both young and old mosquitoes, regardless of wall surface type. Considering delayed mortality, residual efficacies (mosquito mortality equal or greater than 80%) ranged from 1 to ≥12 months, with the duration depending on mortality time post exposure, wall type and mosquito age. Looking at mortality 72h after exposure, residual efficacy was between 6 and 9 months, depending on wall type and mosquito age. Mortality of older mosquitoes was significantly higher on mud-surfaces compared to cemented-surfaces 24h post exposure, but this difference was not significant for the delayed mortalities. The LT50and LT10(i.e. 90% of the mosquitoes survive exposure to the insecticides) values were consistently higher for older mosquitoes using the 24h post-exposure observations and ranged from 0.2 to 5.7 months and 0.2 to 7.2 months for LT50 and LT10, respectively.ConclusionsThe present study highlights the need for assessing mosquito mortality beyond the currently recommended 24h post exposure. Failure to do so may lead to underestimation of the residual efficacy of IRS products, as delayed mortality will lead to a further reduction in mosquito vector populations and potentially negatively impact disease transmission. Monitoring residual efficacy on relevant wall surfaces, including old mosquitoes that are ultimately responsible for malaria transmission, and assessing delayed mortalities are critical to provide accurate and actionable data to guide vector control programmes.
A comparison between CFD and thermal-structural analysis of structural steel members subjected to fire
Authors: Alasiri Muhannad R. , Mahamid Mustafa 
Published: March 2021

Purpose
Standard fire resistance curves such as ASTM E119 have been used for so long in structural fire practice. The issue with use of these curves that they do not represent real fire scenarios. As a result, the alternatives have been to either conduct experiments or find other tools to represent a real fire scenario. Therefore, the purpose of this paper is to understand the temperature effects resulted from a designed fire on steel beams and whether the standard fire curves represent a designed fire scenario.

Design/methodology/approach
Computational fluid dynamics (CFD) models were developed to simulate a designed fire scenario and to understand the structural responses on the beams under elevated temperatures. Consequently, the results obtained from the CFD models were compared with the results of three-dimensional (3D) non-linear finite element (FE) models developed by other researchers. The developed FE models were executed using a standard fire curve (ASTM E119). A parametric study including two case studies was conducted.

Findings
Results obtained from performing this study showed the importance of considering fire parameters such as fuel type and flame height during the thermal analysis compared to the standard fire curves, and this might lead to a non-conservative design as compared to the designed fire scenario. The studied cases showed that the steel beams experienced more degradation in their fire resistance at higher load levels under designed fires. Additionally, the models used the standard fire curves underestimated the temperatures at the early stages.

Originality/value
This paper shows results obtained by performing a comparison study of models used ASTM E119 curve and a designed fire scenario. The value of this study is to show the variability of using different fire scenarios; thus, more studies are required to see how temperature history curves can be used to represent real fire scenarios.
Innovation orientation and its relationship with business performance: moderating role of firm size
Authors: Farooq Rayees , Vij Sandeep , Kaur Jaspreet 
Published: March 2021

PurposeThe study aims to test the relationship between innovation orientation (INO) and business performance. It also explores the moderating effect of firm size on the relationship between INO and business performance.Design/methodology/approachA purposive sample of 278 firms (manufacturing and service) was taken from the National Capital Region and the Punjab state of India. The survey questionnaire was administered to two to three managerial-level employees from each of the 278 firms. Exploratory and confirmatory factor analyses were used to validate the INO and business performance scales. The hypotheses were tested using multi-group moderation analysis and structural equation modeling.FindingsThe study shows that INO has a significant positive effect on business performance. The results have indicated that firm size (based on the number of employees) moderates the relationship between INO and business performance. However, firm size (based on investment) does not moderate the relationship between INO and business performance.Originality/valueThe study is an attempt to synthesize the fragmented results testing innovation–performance relationship using firm size as a moderator on the relationship between INO and business performance and provides insights for both academicians and practitioners.
Malignant pleural mesothelioma in a kidney transplant recipient
Authors: Traipipitsiriwat Wasinee , Songsomboon Chayanon , Junhasavasdikul Detajin , Boonsarngsuk Viboon , Klaisuban Wipawi 
Published: March 2021

AbstractPost‐transplantation malignancy is one of the most common complication‐related mortality in transplant recipients. Here, we report the case of a kidney transplant patient for 2 years with malignant pleural effusion that was subsequently diagnosed as malignant pleural mesothelioma.
Comparison of three different dose regimens of methylprednisolone as initial therapy for pemphigus vulgaris of moderate severity: A single‐center retrospective study from Turkey
Authors: Polat Ekinci Algün , Kiliç Sayar Sıla , Babuna Kobaner Goncagül , Küçükoğlu Rıfkiye 
Published: March 2021

AbstractBackgroundCurrently, no consensus exists on the dosing regimen of corticosteroids in relation to disease severity in pemphigus vulgaris.ObjectiveTo evaluate the efficacy of three initial dose regimens of methylprednisolone in the treatment of moderate pemphigus.MethodsWe retrospectively analysed the data of 46 patients with moderate pemphigus vulgaris, who initially received either low (0.60–0.80 mg/kg/day), moderate (0.81–1.20 mg/kg/day) or high (1.21–2.0 mg/kg/day) doses of methylprednisolone.ResultsThe median time to disease control and hospital stay was shortest in the high‐dose group (8.5 days and 27.5 days, respectively). The low‐dose group had the highest rate of adjuvant therapy (88.8%, P = 0.035). Cumulative methylprednisolone doses and corticosteroid‐related adverse events were similar in the moderate‐ and high‐dose groups. The initial methylprednisolone dose showed a significant inverse correlation with time to disease control (r = −0.319, P = 0.031) and a weak inverse correlation with length of hospital stay (r = −0.282, P = 0.058).LimitationsRetrospective design and small patient size are major limitations.ConclusionIn the treatment of moderate pemphigus vulgaris, high initial doses of corticosteroid seem to provide early disease control and shortened hospital stay without notable increases in cumulative corticosteroid doses. This treatment strategy may lower the risk for nosocomial infections and reduce the economic burden of pemphigus.
Prolactin modifies the in vitro LPS‐induced chemotactic capabilities in human fetal membranes at the term of gestation
Authors: Núñez‐Sánchez Estefanía , Flores‐Espinosa María Del Pilar , Mancilla‐Herrera Ismael , González Leticia , Cisneros José , Olmos‐Ortiz Andrea , Quesada‐Reyna Braulio , Granados‐Cepeda Martha , Zaga‐Clavellina Veronica 
Published: March 2021

AbstractProblemImmune responses of fetal membranes involve the production of chemoattractant mediators causing infiltration of maternal and fetal leukocytes, intrauterine inflammation and potentially the disruption of maternal‐fetal tolerance. Prolactin (PRL) has deep immunoregulatory effects in the fetal‐maternal interface. We aimed to test the in vitro PRL effect upon chemotactic capacities of human fetal membranes.Method of StudyFetal membranes and umbilical cord blood were collected from healthy non‐laboring caesarean deliveries at term. Fetal membranes were cultured in Transwell® frames to mimic the barrier function between choriodecidual and amniotic sides. Tissues were treated with PRL, Lipopolysaccharide (LPS), or both simultaneously. Then, RANTES, MCP‐1, MIP‐1α, IP‐10, and PECAM‐1 were quantified in a conditioned medium by choriodecidual or amniotic sides. The chemotaxis of subsets of migrating mononuclear cells from umbilical cord blood was evaluated in a Boyden Chamber in response to the conditioned medium by both sides.ResultsLipopolysaccharide stimulates the production of RANTES, MCP‐1, MIP‐1α, and PECAM‐1 in choriodecidua, while MIP‐1α and PECAM‐1 only increase in amnion. PRL decrease RANTES, MCP‐1, and MIP‐1 only in choriodecidua, but PECAM‐1 was decreased mainly in amnion. The leukocyte migration was regulated significantly in response to the conditioned medium by the amnion, increase in the conditioned medium after LPS treatment, contrary with, the leukocyte migration decreased in a significant manner in response to conditioned medium after PRL and LPS‐PRL co‐treatment. Finally, T cells were the most responsive subset of cells.ConclusionsProlactin modified in a tissue‐specific manner the chemotactic factor and the leukocyte migration differentially in fetal membranes.
A 3′ tRNA‐derived fragment produced by tRNALeuAAG and tRNALeuTAG is associated with poor prognosis in B‐cell chronic lymphocytic leukemia, independently of classical prognostic factors
Authors: Katsaraki Katerina , Adamopoulos Panagiotis G. , Papageorgiou Sotirios G. , Pappa Vasiliki , Scorilas Andreas , Kontos Christos K. 
Published: March 2021

AbstractObjective3′ tRNA‐derived fragments (3′ tRFs) are important epigenetic regulators in normal and pathological conditions. In this study, we aimed to explore the potential value of a 3′ tRF as a prognostic and/or screening biomarker for B‐cell chronic lymphocytic leukemia (B‐CLL).MethodsPublicly available next‐generation sequencing data from 20 B‐CLL cases were analyzed, followed by prediction of targets of the most abundantly and ubiquitously expressed 3′ tRFs, leading to selection of tRF‐LeuAAG/TAG. PBMCs were isolated from blood samples of 91 B‐CLL patients and 43 non‐leukemic donors, followed by total RNA extraction, in‐vitro polyadenylation, and first‐strand cDNA synthesis. Next, a real‐time quantitative PCR (qPCR) assay was developed for the accurate quantification of tRF‐LeuAAG/TAG and applied in all samples, prior to biostatistical analysis.ResultsHigh tRF‐LeuAAG/TAG levels are associated with inferior overall survival (OS) of B‐CLL patients. The unfavorable significance of tRF‐LeuAAG/TAG was independent of established prognostic factors in B‐CLL. Stratified Kaplan‐Meier OS analysis uncovered the unfavorable prognostic role of high tRF‐LeuAAG/TAG levels for patients in Binet A or Rai I stage, negative CD38 expression, mutated, or unmutated IGHV genomic locus.ConclusionOur approach revealed the independent prognostic value of a particular 3′ tRF, derived from tRNALeuAAG and tRNALeuTAG (tRF‐LeuAAG/TAG) in B‐CLL.
Patients' experiences of behaviour change interventions delivered by general practitioners during routine consultations: A nationally representative survey
Authors: Keyworth Chris , Epton Tracy , Goldthorpe Joanna , Calam Rachel , Armitage Christopher J. 
Published: March 2021

AbstractBackgroundConsistent with the ‘Making Every Contact Count’ UK public health policy, general practitioners (GPs) are expected to provide patients with behaviour change interventions opportunistically. However, there is a belief widely held among GPs that patients neither want or need such interventions. We aimed to understand the following: (a) the characteristics of people attending GP appointments, (b) patients' needs for health behaviour change, (c) perceptions of appropriateness and helpfulness of interventions, and (d) factors associated with recall of receipt of interventions.MethodsCross‐sectional nationally representative online survey of UK adults who had attended GP clinics in the preceding four weeks (n = 3028). Data were analysed using descriptive statistics and binary logistic regression.Results94.5% (n = 2862) of patients breached at least one health behaviour guideline, and 55.1% reported never having had a conversation with their GP about health behaviours. The majority of patients perceived intervention as appropriate (range 84.2%‐87.4% across behaviours) and helpful (range 82.8%‐85.9% across behaviours). Being male (OR = 1.412, 95% CI 1.217, 1.639), having a long‐term condition (OR = 1.514, 95% CI 1.287, 1.782) and a higher number of repeat GP visits (OR = 1.016, 95% CI 1.010, 1.023) were among factors associated with recall of receipt of interventions.ConclusionsPatients perceived behaviour change intervention during routine GP consultations as appropriate and helpful, yet there are variations in the likelihood of receiving interventions according to sociodemographic factors. GPs could adopt a more proactive approach to behaviour change in patient consultations with the broad approval of patients.Patient or public contributionThe questionnaire was piloted among a convenience sample prior to distribution.