bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivodirected evolution of an ultra-fast RuBisCO from a semi-anaerobic environment imparts oxygen resistance
Authors: McDonald Julie L. , Shapiro Nathan P. , Whitney Spencer M. , Wilson Robert H. , Shoulders Matthew D. 
Published: February 2025

AbstractCarbon dioxide assimilation by the enzyme Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (RuBisCO) underpins biomass accumulation in photosynthetic bacteria and eukaryotes. Despite its pivotal role, RuBisCO has a slow carboxylation rate () and is competitively inhibited by oxygen. These traits impose limitations on photosynthetic efficiency, making RuBisCO a compelling target for improvement. Interest in RuBisCO fromGallionellaceaebacteria, which comprise a dimer or hexamer of large subunits, arises from their nearly 5-fold higherthan the average RuBisCO enzyme. Here, a comprehensive kinetic analysis of aGallionellaceaeRuBisCO (GWS1B) reveals the fastest CO2fixation rate measured for this enzyme (25.8 s−1), and exposes an extreme sensitivity to oxygen inhibition, consistent with its evolution under semi-anaerobic environments. We used a novelin vivomutagenesis-mediated screening pipeline to evolve GWS1B over six rounds under oxygenic selection, identifying three catalytic point mutants with improved ambient carboxylation efficiency; Thr-29-Ala (T29A), Glu-40-Lys (E40K) and Arg-337-Cys (R337C). Full kinetic characterization showed that each substitution enhanced the CO2affinity of GWS1B under oxygenic conditions while simultaneously subduing oxygen affinity, leading to 24% (E40K), 9% (T29A) and 7% (R337C) enhancements in carboxylation efficiency under ambient O2. By contrast, under the near anaerobic natural environment ofGallionellaceae, the carboxylation efficiency of each mutant was impaired ∼16%. These findings demonstrate the efficacy of artificial directed evolution to access novel regions of catalytic space in RuBisCO, and a capacity for rapid evolution of kinetic traits in response to environmental change.SignificanceGiven RuBisCO’s crucial role in carbon dioxide assimilation, addressing its slow carboxylation rate and oxygen inhibition is a significant challenge. Utilizing the fastest known, yet highly oxygen-sensitive, RuBisCO from the bacteriaGallionellaceae, we applied a novelin vivodirected evolution pipeline inEscherichia colito discover mutations that specifically enhance carboxylation efficiency under ambient oxygen, a condition distinct fromGallionellaceae’snatural semi-anaerobic environment. Our findings underscore the potential of directed evolution to unlock new catalytic capabilities for RuBisCO, with implications for both fundamental research and practical agricultural applications.
Biological function and mechanism offlpLgene in regulating pathogenicity ofAeromonas hydrophila
Authors: Jiao Hanyang , Liu Kemei , Liao Ziyi , Naseer Adeeba , Ye Hua , Xu Hao , Li Yun , Yu Yongyao , Mei HuiQing , Wu Ronghua 
Published: February 2025

ABSTRACTAeromonas hydrophilais human-animal commensal bacterium that seriously threatens the development of aquaculture and human health. Type IV pili (T4P) are essential for bacterial physiological function. The relationship between the molecular mechanism offlpLgene affiliated to T4P and the pathogenicity is still unclear. In the research,A. hydrophilawith a genetically stable deletion offlpLgene (ΔflpL) was constructed. The median lethal dose of ΔflpLfor Crucian carp was 4.87 times higher compared to wild-type strain, suggesting that ΔflpLsignificantly reduced pathogenicity. The attenuation may be attributed to a reduced capacity for biofilm formation and downregulation of the expression levels of virulence-related genes in ΔflpL. Meanwhile, the significant increase in swimming capacity and adhesion can be related to the upregulation offlpC, tapM, tapBandtapPgenes. In conclusion,flpLgene plays essential role in the pathogenicity, adhesion, motility, and capacity for biofilm formation inA. hydrophila. The research aims to clarify the pathogenesis ofA. hydrophila, and lay the foundation for developing live attenuated vaccines through the targeted modificationflpLgene.
A comparison of short- and long-read whole genome sequencing for microbial pathogen epidemiology
Authors: Schiffer Andrea M. , Rahman Arafat , Sutton Wendy , Putnam Melodie L. , Weisberg Alexandra J. 
Published: February 2025

AbstractWhole genome sequencing provides the highest resolution for characterizing pathogen evolution, epidemiology, and diagnostics. Genome assemblies contain information on the identity and potential phenotypes of a pathogen. Likewise, variant calling can inform on transmission patterns and evolutionary relationships. Recent improvements in Oxford Nanopore long-read sequencing have made its use attractive for genomic epidemiology. However, the accuracy and optimal strategy for analysis of Nanopore reads remains to be determined. We compared the use of Illumina short reads and Oxford Nanopore long reads for genome assembly and variant calling of phytopathogenic bacteria. We generated short- and long-read datasets for diverse phytopathogenicAgrobacteriumstrains. We then analyzed these data using multiple pipelines designed for either short or long reads and compared the results. We found that assemblies made from long reads were more complete than those made from short-read data and contained few sequence errors. Variant calling pipelines differed in their ability to accurately call variants and infer genotypes from long reads. Results suggest that computationally fragmenting long reads can improve the accuracy of variant calling in population-level studies. Using fragmented long reads, pipelines designed for short reads were more accurate at recovering genotypes than pipelines designed for long reads. Further, short- and long-read datasets can be analyzed together with the same pipelines. These findings show that Oxford Nanopore sequencing is accurate and can be sufficient for microbial pathogen genomics and epidemiology. Ultimately, this enhances the ability of researchers and clinicians to understand and mitigate the spread of pathogens.
Key drivers affecting safety intervention success: evidence from Thai construction industry
Authors: Kurakaew Nuanan , Hadikusumo Bonaventura 
Published: February 2025

PurposeSafety interventions are a crucial component of organizational strategy, which are aimed at mitigating factors that compromise workplace safety. In the construction industry, these interventions are vital owing to the high-risk nature of construction activities. For developing effective safety interventions, the underlying factors that drive the need for these interventions must be understood. This study identifies and analyzes the critical factors driving safety interventions in Thai construction projects, focusing on both situational and organizational factors.Design/methodology/approachA survey conducted with a questionnaire among medium- to large-sized building contractors in Bangkok and nearby areas garnered 98 project responses. To validate the constructs of reasons for safety interventions and identify critical safety practices, the data were analyzed using confirmatory factor analysis and structural equation modeling.FindingsThe results indicate that organizational factors, positively influenced by situational factors, have a direct effect on safety interventions. Successful safety interventions can be obtained by focusing on five key organizational factors: safety as a core value, continuous improvement plans, leadership and management support, safety strategies and policies and safety committees.Originality/valueThese results provide valuable insights for construction site management, providing guidance on improving safety interventions via the effective utilization of these critical factors.
Exploring the dynamics of student learning behavior in higher education: an expanded theory of reasoned action approach
Authors: Bhojak Nimesh P. , Momin Mohammadali , Joshi Brijeshkumar H. 
Published: February 2025

PurposeThis research aims to enhance the model of student learning behavior by expanding the traditional theory of reasoned action (TRA) to incorporate variables such as student mental well-being (SMW), attitude, subjective norms (SNB), perceived behavioral control (PBC) and climate change concerns. The study examines students’ intentions as a mediator variable and student area as a moderator variable in student learning behaviors.Design/methodology/approachA structured questionnaire was administered to 610 higher education students in Gujarat, exploring variables related to student characteristics, mental well-being, attitudes, SNB, PBC, Climate change concerns (CML), change concerns and intentions to study. Data analysis involved descriptive statistics, exploratory factor analysis and structural equation modeling with mediation and moderation analyses using SPSS 22 and AMOS 26.FindingsThe empirical findings support the hypothesized relationships within the expanded model, indicating significant contributions of SMW, attitude, SNB, PBC and CML to forming solid intentions to pursue education. Additionally, the study reveals a positive association between intention and subsequent learning behavior moderated by the student residence area.Research limitations/implicationsThe study confirms the proposed relationships within the expanded model, with SMW significantly influencing students’ academic intentions. Factors like attitude, SNB and climate change are crucial in shaping educational pursuits. The research enriches educational psychology by detailing the complex influences on student learning behavior, offering insights for enhancing educational strategies and outcomes as the academic environment evolves.Originality/valueThis research offers an innovative approach by expanding the traditional TRA to explore the influences on student learning behavior in education comprehensively. Integrating variables such as SMW, attitude and CML provides a nuanced understanding, contributing to theoretical development and practical educational implications.
Smart living services’ factors and influences on subjective well-being and intention to use
Authors: Lu Hsiao-Han , Huang Wei-Jen 
Published: February 2025

Purpose
This study aims to examine the effects of network externalities and diffusion of innovation on users’ perceived usefulness and perceived enjoyment, and in turn subjective well-being and intention to use in the smart living context.

Design/methodology/approach
To explore different types of smart living services, the data collected come from surveying 512 users of smart home services and 570 users of smart living apps. Structural equation modeling is used to analyze the data, and post hoc interviews are conducted to provide insights into our conceptual model of smart living services.

Findings
Compatibility and perceived service complementarity are the most influential determinants of users’ perceived usefulness and perceived enjoyment toward smart living services. Perceived usefulness has a greater impact on users’ intention to use and their subjective well-being than perceived enjoyment. Interestingly, perceived enjoyment exerts a stronger influence on subjective well-being than on intention to use.

Originality/value
This study is one of the first to provide empirical evidence in the context of smart living services, contributing to transformative service literature by extending the understanding of technology use and its influence on user well-being into a less explored service context. This study also advances users’ intention to use and subjective well-being of technology adoption in the service context by integrating perspectives from network externalities and diffusion of innovation.
Stakeholder activities in PCAOB audit standard setting: a manual content analysis of comment letter submissions
Authors: Yang Xiaoshuai , Zhang Joseph H. 
Published: February 2025

Purpose
The purpose of this study is to analyze comment letters on Docket 034, which proposes reforms to audit reports, to shed light on the actions of various stakeholder groups in the Public Company Accounting Oversight Board (PCAOB)’s standard-setting process. The study identifies stakeholder groups, examines the timing of their involvement, and evaluates their arguments and lobbying efforts to influence regulatory outcomes.

Design/methodology/approach
This paper examines stakeholders’ behaviors in submitting comment letters during the PCAOB’s public consultation period for its rulemaking and their influence on regulatory outcomes. Stakeholder participation in the PCAOB’s rulemaking process is analyzed through a content analysis of 489 comment letters submitted to PCAOB’s Docket 034, using manual content analysis. Stakeholder groups are identified, including accounting professionals, financial statement preparers, financial statement users, regulators and academics. Argument strategies, such as economic, conceptual or a combination of both approaches, are also identified.

Findings
This paper finds that accounting professionals and preparers submit more comment letters than the other stakeholder groups. There is a significant association between stakeholder groups and their lobbying position across issues. Preparers take negative positions on all issues except independence, whereas users support all issues. There is also a significant association between stakeholder groups and their argument strategies, and the significant differences lie in preparers and accounting professionals. Preparers primarily rely on economic arguments, such as costs or time. At the same time, accounting professionals use economic and conceptual arguments (such as violating the FASB conceptual framework) to justify their positions.

Originality/value
To the best of the authors’ knowledge, this is the first study to examine lobbying in the PCAOB’s due process, including all stakeholder groups. The PCAOB’s audit standard-setting process and public consultation via the online docket system promote transparency and the effectiveness of public participation. This study analyzes the nature and impact of stakeholder participation in this due process. Understanding lobbying in an audit standard setting enables the PCAOB and stakeholder groups to work more efficiently on regulatory reforms. This assessment of lobbying efforts in the USA complements and enriches the growing body of literature on lobbying efforts and audit policy reforms across countries. Second, by extending the current literature, this study examines the complexities of lobbying through a detailed content analysis of comment letters. This approach provides insights into the properties of lobbying about stakeholder groups, the scope of lobbying, argument strategies, opinions and consultation periods.
Adapting to the digital marketplace: manufacturer channel selection in the age of consumer migration
Authors: Zhu Jing , Nan Xingchen , Tan Adrian Chen Yang , Wu Fen 
Published: February 2025

Purpose
This study aims to examine manufacturers’ strategic responses to consumer migration from offline to online channels, focusing on how these shifts affect their channel selection and business strategies.

Design/methodology/approach
This research uses a theoretical framework using a Stackelberg game model to analyze manufacturers’ decision-making processes amid evolving consumer behaviors. It intricately explores the strategic implications across three distinct channel structures: manufacturer direct sales (MD), retailer resale (RR) and retailer agency (RA), focusing on their economic outcomes and market dynamics. This approach is instrumental in decoding the multifaceted nature of channel migration and its impact on manufacturer–retailer relationships in the digital marketplace.

Findings
The research reveals that in MD and RA scenarios, as channel migration intensifies, manufacturers tend to lower both wholesale and online retail prices. Conversely, in the RR scenario, the set wholesale price is intricately linked to the market share, with higher prices set for smaller offline market shares. From a strategic standpoint, MD emerges as the optimal choice for maximizing manufacturer profits, while RA takes precedence when considering the entire supply chain’s profitability, particularly under high commission costs.

Originality/value
This research illuminates the impact of channel migration on manufacturers’ pricing strategies and channel selection. It not only advances the understanding of consumer behavior in multichannel retail environments but also offers practical insights for businesses in effectively managing online and offline channels.
Mothers against the natural order: Gender representations and desertion of identities in the drama of disinheriting a son in eighteenth‐century Barcelona
Authors: Fargas Peñarrocha Mariela 
Published: February 2025

ABSTRACTThe disinheritance of a firstborn son accustomed to the privileges of exclusion has for centuries been a dramatic event for families, especially if the decision was taken by a woman, the son's own mother. Very few dared to do so, because it symbolised a break with the notion of virtuous, compassionate motherhood; it represented a failure to be ashamed of, as well as being a bold challenge against the male line. Through examination of litigation documents, this text describes a case of disinheritance by a mother that occurred in Barcelona in 1732. The analysis considers the different perspectives of those involved in such events, from the struggle over gender representations to small acts of self‐affirmation by women who became agents of their own lives.
Agonistic interactions between Vespula vulgaris (Linnaeus) and Vespula germanica (Fabricius) (Hymenoptera: Vespidae) during foraging
Authors: Manattini María Celeste , Lozada Mariana , Buteler Micaela 
Published: February 2025

AbstractForaging behavior is a key factor associated with the success of social insect invasions. Vespula wasps show complex behavioral patterns and social mechanisms associated with foraging, which are directly related to their invasive success in several countries worldwide. Vespula vulgaris (Linnaeus) and Vespula germanica (Fabricius) are invasive wasps, coexisting in Patagonia, showing temporal and dietary overlap. As generalist, opportunistic predators and scavengers with broad diets, these sympatric wasps share similar niches and foraging habits. We analyzed their foraging strategies and interaction, observing the behavior of each species in the presence of workers of the same species, and the other species at a given resource, directly and indirectly. Directly, from a continuous visual record, we observed and recorded the frequency of wasp's behavior at a feeder for 30 min. Indirectly, we conducted pairwise choice tests to compare wasp preference for treated and untreated feeders with visual and olfactory cues simulating the presence of wasps of the same or different species. We found consistent differences between species in aggressiveness. V. vulgaris showed a greater degree of intraspecific agonistic behavior than toward V. germanica, while V. germanica was less aggressive, and intraspecific and interspecific interactions were similar. Also, V. vulgaris preferred landing on baits without visual and olfactory cues simulating wasps presence, while V. germanica preferred baits with cues simulating its own species over baits with cues representing V. vulgaris. Our results suggest that V. germanica prioritize social facilitation as foraging strategy, while for V. vulgaris aggression is the predominant strategy.
Comparison of all types of loop‐diuretics for chronic heart failure: A protocol for a systematic review and network meta‐analysis
Authors: Taleghani Sami , Lukoschewitz Jasmin Dam , Tonning Sandra , Taraldsen Ida Arentz , Jakobsen Janus C. , Grand Johannes 
Published: February 2025

AbstractBackgroundPatients with chronic heart failure (CHF) experience congestion‐related symptoms such as dyspnoea and oedema, both of which are associated with poor outcomes. Loop diuretics are the cornerstone to treat congestion and to maintain euvolemia. While furosemide is the most used loop diuretic, other loop diuretics may exhibit theoretical advantages. We aim to compare all types of loop diuretics for patients with CHF.MethodsThis protocol for a systematic review is conducted with guidance from the Cochrane Handbook and the Preferred Reporting Items for Systematic Reviews and Meta‐Analysis Protocols. We will include randomised clinical trials (RCTs) of loop diuretics with other loop diuretics, placebo, or standard of care in CHF patients. The search will be conducted across the major medical databases (including Medline, Embase, and Cochrane Central Register of Controlled Trials). The searches will begin in February 2025. The primary outcome will be all‐cause mortality. Secondary outcomes will be all‐cause hospitalization, serious adverse events (SAEs), and changes in body weight (kg). Data will be analysed by traditional meta‐analyses, Trial Sequential Analyses (TSA), and network meta analysis. The risk of bias will be assessed using the Cochrane Risk of Bias tool—version 2. The certainty of the evidence will be assessed using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) and Confidence in Network Meta‐Analysis (CINEMA) approach.
Gastrointestinal function outcomes following radical and conservative colorectal surgery for deep endometriosis: A systematic review and meta‐analysis
Authors: Darici Ezgi , Bokor Attila , Pashkunova Daria , Senft Birgit , Cimşit Nilüfer , Hudelist Gernot 
Published: February 2025

AbstractIntroductionPatients who have undergone colorectal surgery for symptomatic deep endometriosis may still encounter persistent or worsening digestive complaints. The aim of the present work was to analyze gastrointestinal function outcomes after radical and conservative colorectal surgery to further elucidate the effect of surgery on postoperative bowel function.Material and MethodsPubMed, EMBASE, Web of Science, Clinical Trials.gov and the Cochrane Database databases were searched from January 1, 2010 until April 1, 2024. The quality of included studies was assessed by the Downs and Black quality checklist. Studies including patients with colorectal endometriosis who either underwent segmental resection (SR) or conservative approaches and reported data on bowel function were included.ResultsFrom the initial pool of 55 studies, 14 reported patient reported outcome measures eligible to be pooled in the meta‐analysis. Conservative surgery was less associated with constipation and increased number of daily stool (>3/day) when compared to SR (p = 0.02 and p = 0,0004, respectively). No difference was found in the occurrence of gas and stool incontinence (p = 0.72), postsurgical defecation pain (p = 0.44) and time to defer defecation (≤ 15 min; p = 0.64). Patients in the conservative surgery group reported higher postoperative Gastrointestinal Quality of Life Index (GIQLI) when compared to SR (p = 0.01). However, when comparing changes between pre‐ and postsurgical patient reported outcome measures within the respective groups, rather than evaluating postsurgical outcomes alone, none of the intervention groups showed significant changes between pre‐ and postsurgical GIQLI, Knowles Eccersley Scott Symptom Score(KESS) and Wexner scores (p = 0.28, p = 0.94 and p = 0.78, respectively).ConclusionsSegmental resection seems to be associated with higher rates of post‐operative constipation and lower GIQLI scores when compared to conservative surgery. However, when comparing the change of gastrointestinal function symptoms reflected by changes of gastrointestinal function parameters from pre‐ to postoperative rather than focusing on purely postoperative parameters alone, no significant difference of these parameters was observed between surgical techniques.