bluuebunny/crossref_metadata_2025_split huggingface dataset:
A belt for the cell: cellulosic wall thickenings and their role in morphogenesis of the 3D puzzle cells in walnut shells
Authors: Antreich Sebastian J , Xiao Nannan , Huss Jessica C , Gierlinger Notburga 
Published: May 2021

AbstractWalnut (Juglans regia) kernels are protected by a tough shell consisting of polylobate sclereids that interlock into a 3D puzzle. The shape transformations from isodiametric to lobed cells is well documented for 2D pavement cells, but not for 3D puzzle sclereids. Here, we study the morphogenesis of these cells by using a combination of different imaging techniques. Serial face-microtomy enabled us to reconstruct tissue growth of whole walnut fruits in 3D, and serial block face-scanning electron microscopy exposed cell shapes and their transformation in 3D during shell tissue development. In combination with Raman and fluorescence microscopy, we revealed multiple loops of cellulosic thickenings in cell walls, acting as stiff restrictions during cell growth and leading to the lobed cell shape. Our findings contribute to a better understanding of the 3D shape transformation of polylobate sclereids and the role of pectin and cellulose within this process.
Training Program for Ultrasound-Guided Intravenous Catheter Insertion
Authors: Filipovich Samantha J , Dilgard John W , Conrad Simon P , Moore Chad B , Hefley Justin B 
Published: May 2021

ABSTRACT

Introduction
Ultrasound guidance is now widely available in military treatment facilities and civilian hospitals alike, both in the USA and in forward-deployed military environments. Technical mastery of ultrasound-guided peripheral intravenous (USGPIV) catheter insertion can be easily achieved through a short training course. Mastery can be achieved even when trainees have a limited medical background before course attendance. An evidence-based practice project team sought to improve the knowledge, confidence, and skills in the placement of USGPIV catheters by clinicians at Naval Hospital Jacksonville. Completion of an USGPIV training program can equip healthcare providers with knowledge and confidence for placement of peripheral access necessary in critical situations such as those requiring medications or blood products.

Materials and Methods
The project team conducted a literature review to evaluate the appropriateness of USGPIV training for the nurses and military medical technicians in this setting. The team developed and delivered a USGPIV training program based on adaptations from the literature. During the training period, knowledge and confidence scores were reported by each trainee to evaluate the perceptions of the quality of training. The number of attempted catheter placements and ultrasound utilization was recorded in the pre- and post-implementation periods to evaluate the project’s effect on the delivery of patient care. Statistical analysis was conducted to evaluate project outcomes.

Results
In the pre-intervention period, none of the 252 intravenous catheters were placed with the USGPIV technique, compared to 50 of 267 in the post-intervention period. These results demonstrate an 18.7% increase in the USGPIV access approach by nursing staff. Mean knowledge scores significantly increased following the delivery of the training, 60% versus 80% in the pre- and post-training assessments, respectively (P &lt; .001). Mean self-reported skill confidence scores also significantly improved (P &lt; .001).

Conclusion
Knowledge and self-reported confidence in USGPIV access improved for the trainees. Mean knowledge improved from 60% to 80%, while mean confidence scores increased from 2.74 to 3.79 for corpsman and from 3.0 to 3.88 for nurses. Utilization of the USGPIV technique increased by 18.7% in the post-intervention period. These results demonstrate that implementing this training program can improve knowledge, confidence, and use of ultrasound during the placement of PIV catheters.
Chromospheric activity in 55 Cancri: II. Theoretical wave studies versus observations
Authors: Cuntz Manfred , Schröder Klaus-Peter , Fawzy Diaa E , Ridden-Harper Andrew R 
Published: May 2021

ABSTRACT
In this study, we consider chromospheric heating models for 55 Cancri in conjunction with observations. The theoretical models, previously discussed in Paper I, are self-consistent, non-linear, and time-dependent ab-initio computations encompassing the generation, propagation, and dissipation of waves. Our focus is the consideration of both acoustic waves and longitudinal flux tube waves amounting to two-component chromosphere models. 55 Cancri, a K-type orange dwarf, is a star of low activity, as expected by its age, which also implies a relatively small magnetic filling factor. The Ca ii K fluxes are computed (multi-ray treatment) assuming partial redistribution and time-dependent ionization. The theoretical Ca ii H+K fluxes are subsequently compared with observations. It is found that for stages of lowest chromospheric activity the observed Ca ii fluxes are akin, though not identical, to those obtained by acoustic heating, but agreement can be obtained if low levels of magnetic heating – consistent with the expected photospheric magnetic filling factor – are considered as an additional component; this idea is in alignment with previous proposals conveyed in the literature.
Wavelet Screening identifies regions highly enriched for differentially methylated loci for orofacial clefts
Authors: Denault William R P , Romanowska Julia , Haaland Øystein A , Lyle Robert , Taylor Jack A , Xu Zongli , Lie Rolv T , Gjessing Håkon K , Jugessur Astanand 
Published: May 2021

AbstractDNA methylation is the most widely studied epigenetic mark in humans and plays an essential role in normal biological processes as well as in disease development. More focus has recently been placed on understanding functional aspects of methylation, prompting the development of methods to investigate the relationship between heterogeneity in methylation patterns and disease risk. However, most of these methods are limited in that they use simplified models that may rely on arbitrarily chosen parameters, they can only detect differentially methylated regions (DMRs) one at a time, or they are computationally intensive. To address these shortcomings, we present a wavelet-based method called ‘Wavelet Screening’ (WS) that can perform an epigenome-wide association study (EWAS) of thousands of individuals on a single CPU in only a matter of hours. By detecting multiple DMRs located near each other, WS identifies more complex patterns that can differentiate between different methylation profiles. We performed an extensive set of simulations to demonstrate the robustness and high power of WS, before applying it to a previously published EWAS dataset of orofacial clefts (OFCs). WS identified 82 associated regions containing several known genes and loci for OFCs, while other findings are novel and warrant replication in other OFCs cohorts.
The 2021 WHO Classification of Tumors of the Central Nervous System: a summary
Authors: Louis David N , Perry Arie , Wesseling Pieter , Brat Daniel J , Cree Ian A , Figarella-Branger Dominique , Hawkins Cynthia , Ng H K , Pfister Stefan M , Reifenberger Guido , Soffietti Riccardo , von Deimling Andreas , Ellison David W 
Published: May 2021

AbstractThe fifth edition of the WHO Classification of Tumors of the Central Nervous System (CNS), published in 2021, is the sixth version of the international standard for the classification of brain and spinal cord tumors. Building on the 2016 updated fourth edition and the work of the Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy, the 2021 fifth edition introduces major changes that advance the role of molecular diagnostics in CNS tumor classification. At the same time, it remains wedded to other established approaches to tumor diagnosis such as histology and immunohistochemistry. In doing so, the fifth edition establishes some different approaches to both CNS tumor nomenclature and grading and it emphasizes the importance of integrated diagnoses and layered reports. New tumor types and subtypes are introduced, some based on novel diagnostic technologies such as DNA methylome profiling. The present review summarizes the major general changes in the 2021 fifth edition classification and the specific changes in each taxonomic category. It is hoped that this summary provides an overview to facilitate more in-depth exploration of the entire fifth edition of the WHO Classification of Tumors of the Central Nervous System.
Pipeline Embolization Devices for the Treatment of Intracranial Aneurysms, Single-Center Registry: Long-Term Angiographic and Clinical Outcomes from 1000 Aneurysms
Authors: Lylyk Ivan , Scrivano Esteban , Lundquist Javier , Ferrario Angel , Bleise Carlos , Perez Nicolas , Lylyk Pedro Nicolas , Viso Rene , Nella-Castro Rodolfo , Lylyk Pedro 
Published: May 2021

Abstract

BACKGROUND
Prospective studies have established the safety and efficacy of the PipelineTM Embolization Device (PED; Medtronic) for treatment of intracranial aneurysms (IA).

OBJECTIVE
To investigate long-term outcomes from the Pipeline Embolization Devices for the Treatment of Intracranial Aneurysms (PEDESTRIAN) Registry.

METHODS
The PEDESTRIAN Registry data were retrospectively reviewed, which included patients (March 2006 to July 2019) with complex IAs treated with PED. Patients with unfavorable anatomy and/or recurrence following previous treatment were included and excluded those with acute subarachnoid hemorrhage. The primary angiographic endpoint was complete occlusion and long-term stability. Clinical and radiological follow-up was performed at 3 to 6 mo, 12 mo, and yearly thereafter.

RESULTS
A total of 835 patients (mean age 55.9 ± 14.7 yr; 80.0% female) with 1000 aneurysms were included. Aneurysms varied in size: 64.6% were small (≤10 mm), 25.6% were large (11-24 mm), and 9.8% were giant (≥25 mm). A total of 1214 PEDs were deployed. Follow-up angiography was available for 85.1% of patients with 776 aneurysms at 24.6 ± 25.0 mo (mean). Complete occlusion was demonstrated in 75.8% of aneurysms at 12 mo, 92.9% at 2 to 4 yr, and 96.4% at >5 yr. During the postprocedural period, modified Rankin Scale scores remained stable or improved in 96.2% of patients, with stability or improvement in 99.1% of patients >5 yr. The overall major morbidity and neurological mortality rate was 5.8%.

CONCLUSION
This study demonstrated high rates of long-term complete aneurysm occlusion, stable or improved functional outcomes, and low rates of complications and mortality. Clinical and angiographic outcomes improved over long-term follow-up, demonstrating that endovascular treatment of IA with PED is safe and effective.
Properties of the accretion ring in an X-ray binary, and accretion and excretion two-layer flows from it
Authors: Inoue Hajime 
Published: May 2021

Abstract
We study properties of an accretion ring in a steady mass flow from a companion star to a compact object in an X-ray binary. The accretion ring is a place where matter inflowing from a companion star sojourns for a while to bifurcate to accretion and excretion flows due to angular momentum transfer. The matter in the accretion ring rotates along the Keplerian circular orbit determined by the intrinsic specific angular momentum of the inflowing matter and forms a thick ring-envelope. Two internal flows are expected to appear in the thick envelope. One is a mass-spreading flow bifurcating to a thick accretion flow and a thick excretion flow, as a result of the angular momentum transfer within the ring-envelope. The other is a cooling flow toward the envelope center governed by radiative cooling under the effect of X-ray irradiation. This cooling flow eventually forms a core in the torus, from which a thin accretion disk and a thin excretion disk spread out, again as a result of the angular momentum transfer there. Evaluating and comparing the timescales for the two internal flows, the accretion ring is shown to generally originate a two-layer accretion flow in which a thin accretion disk is sandwiched by a thick accretion flow, unless the accretion rate is very low. Properties of the thin excretion disk and the thick excretion flow are also investigated. The thin excretion disk is expected to terminate at a distance of four times the accretion ring radius and to form another ring there, unless tidal effects from the companion star exist. The thick excretion flow is, on the other hand, likely to turn into a supersonic wind flow reaching infinity.
Revealing the architecture of the photosynthetic apparatus in the diatom Thalassiosira pseudonana
Authors: Arshad Rameez , Calvaruso Claudio , Boekema Egbert J , Büchel Claudia , Kouřil Roman 
Published: May 2021

Abstract
Diatoms are a large group of marine algae that are responsible for about one-quarter of global carbon fixation. Light-harvesting complexes of diatoms are formed by the fucoxanthin chlorophyll a/c proteins and their overall organization around core complexes of photosystems (PSs) I and II is unique in the plant kingdom. Using cryo-electron tomography, we have elucidated the structural organization of PSII and PSI supercomplexes and their spatial segregation in the thylakoid membrane of the model diatom species Thalassiosira pseudonana. 3D sub-volume averaging revealed that the PSII supercomplex of T. pseudonana incorporates a trimeric form of light-harvesting antenna, which differs from the tetrameric antenna observed previously in another diatom, Chaetoceros gracilis. Surprisingly, the organization of the PSI supercomplex is conserved in both diatom species. These results strongly suggest that different diatom classes have various architectures of PSII as an adaptation strategy, whilst a convergent evolution occurred concerning PSI and the overall plastid structure.
Advocating for Medicaid Expansion in Republican States: Overcoming “Fractious Federalism” in the Statehouse and Ballot Box
Authors: Haselswerdt Jake 
Published: May 2021

AbstractPartisan and ideological polarization have been major barriers to the implementation of the Affordable Care Act’s Medicaid expansion in Republican-controlled states. Scholars have referred to this situation as “fractious federalism,” with Republican state policymakers toeing the national party line in refusing to cooperate with a major policy initiative. In some cases, however, diverse advocacy coalitions have overcome fractious federalism to pass expansion legislation in deeply Republican states. More recently, such coalitions have resorted to ballot initiative campaigns as another means of overcoming such polarization, and won impressive victories in a series of “deep red” states. Drawing on forty-four interviews with people involved in expansion advocacy in eleven states, I report important insights on the formation and activities of these coalitions in both the legislative and ballot initiative eras of Medicaid expansion politics.
Disproportionate multimorbidity among veterans in middle age
Authors: Bloeser Katharine , Lipkowitz-Eaton Jennifer 
Published: May 2021

Abstract

Background
Little is known about the prevalence of multimorbidity among middle-aged veterans. Multimorbidity holds implications for planning for a population with high health care utilization, poor quality of life and marked need for interdisciplinary care.

Methods
The current study used the US 2017 Behavior Risk Factor Surveillance System to measure multimorbidity in three ways: (1) reporting two or more health conditions, (2) reporting two or more conditions controlling for demographic characteristics (e.g. income) and health risk behaviors (e.g. smoking) and (3) a weighted index using health-related quality of life.

Results
After age 25, veterans’ risk for multimorbidity increased across all age groups. The increased odds of reporting multimorbidity was highest when comparing veterans aged 35–44 to non-veterans of the same ages. Veterans aged 35–44 are 50% (adjusted odds ratios (AOR) 1.50, 95% confidence interval (CI) 1.16, 1.94) to 80% (AOR 1.80, 95% CI 1.46, 2.23) more likely to report multimorbidity when compared with same aged non-veterans.

Conclusions
Younger veterans may benefit from comprehensive interdisciplinary services to aid in the treatment of multiple medical conditions. Failure to account for the impact of chronic conditions on quality of life may lead to an underestimate of the health care needs of veterans across the lifespan.
004 Samelisant (SUVN-G3031), Differentiating features over current treatments of narcolepsy
Authors: Shinde Anil , Subramanian Ramkumar , Palacharla Raghava , Benade Vijay , Abraham Renny , Kamuju Venkatesh , Pandey Santosh , Badange Rajesh , Achanta Pramod Kumar , Nirogi Ramakrishna 
Published: May 2021

Abstract

Introduction
Majority of pharmacological agents used in the treatment of narcolepsy have several limitations. Both nonclinical and clinical evidences suggest usefulness of the histamine H3 receptor (H3R) inverse agonists for the treatment of narcolepsy addressing several of the current limitations.

Methods
Extensive nonclinical studies were carried out for Samelisant (SUVN-G3031) and other pharmacological agents that are currently being used for the treatment of narcolepsy. The nonclinical parameters like inter-species binding affinity, selectivity profile, in vivo and in vitro ADME features, nonclinical efficacy, neurochemistry and safety were compared.

Results
Samelisant has no inter-species variation in binding affinity at H3R with less than 50% inhibition at 1 µM against 70 other targets. Unlike pitolisant, Samelisant has no significant binding affinity at sigma 1 and 2 receptor. Samelisant has no inhibition and induction liability towards major CYP enzymes and transporters. Pitolisant is reported to be a CYP3A4, CYP2B6, and CYP1A2 inducer and a CYP2D6 and OCT1 inhibitor. Samelisant has robust wake promoting effects. Samelisant showed negligible affinity towards hERG channel with IC50 &gt; 10 µM and had no effects on heart rate or ECG parameters in dog telemetry study. Samelisant did not show convulsion in rats up to the tested dose of 100 mg/kg, p.o. Most of the pharmacological agents used for the treatment of narcolepsy have abuse liability; Samelisant produced no change in the striatal and accumbal dopamine levels in rats suggesting no propensity to induce abuse liability. Unlike competing H3R inverse agonists, Samelisant has no effects on fertility and embryo-fetal development up to the highest tested doses.

Conclusion
Nonclinical studies demonstrated superiority of Samelisant over pharmacological agents used in the treatment of narcolepsy. Samelisant is currently being evaluated in a Phase 2 study as monotherapy for the treatment of narcolepsy with and without cataplexy (ClinicalTrials.gov Identifier: NCT04072380).
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011 Shiftwork, Functional Bowel Symptoms and the Microbiome
Authors: Rogers Ann , Hu Yijuan , Yue Ye , Wessel Emily , Christie Jennifer , Read Timothy 
Published: May 2021

Abstract

Introduction
There are about 15 million Americans working full-time on evening, night, or rotating shifts. Between 48% and 81.9% of those working rotating or night shifts report abdominal pain, constipation, diarrhea and other symptoms of functional bowel disorders. The basis for this high prevalence of functional bowel disorders, including irritable bowel syndrome (IBS), among shift workers is unknown. Animal studies, however, suggest that circadian disruption, similar to that in shift workers, may contribute to the development of GI complaints among shift workers by altering the composition and normal diurnal rhythmicity of the resident intestinal microbes.

Methods
Fifty-one full time staff nurses who worked either 12-hour day or night shifts completed demographic information, the Rome III IBS module, daily symptoms diaries for 14 days. They also collected two samples of gut microbiota before the beginning and at the end of their last work shift on day 14, using validated field-tested methods consistent with the Human Microbiome Project. After DNA extraction, 16S rRNA sequencing and assignment to the genus level was completed, samples were then compared to determine if there were 1) differences in the diversity and profile of the microbiome by shift type; 2) if there were differences in the microbiome by time of day for collection; and 3) whether there were differences in the diversity and profile of the microbiome of nurses with IBS and those without IBS.

Results
There were no differences in alpha or beta diversity of gut microbiota when specimens from day and night shift nurses were compared. There were however marginal differences in beta diversity when specimens collected at the beginning and end of the shifts were compared, with seven OTUs being differentially abundant when collected from day shift workers in the evening. There were also three OTUs to be differentially abundant in participants reporting IBS symptoms.

Conclusion
These data did not reveal strong effects of circadian alterations in gut microbiota related to shiftwork. Other factors, such as insufficient sleep and dietary intake during the data-gathering period, need to be explored

Support (if any)
Emory University Synergy Grant Program
018 The Role of Inflammatory Markers in Sleep in Individuals Post-Myocardial Infarction
Authors: Hirsch Sophie , Gaultney Jane , Crane Patricia 
Published: May 2021

Abstract

Introduction
Despite positive secondary prevention strategies post myocardial infarction (MI), including statin use and lifestyle changes, 32% of the annual MIs are recurrent (MIR). As coronary heart disease is related to atherosclerosis, a chronic inflammatory process, and sleep is associated with cardiovascular disease and innate immunity, understanding the role of sleep and inflammation and MIR is important in developing interventions to improve sleep, reduce inflammation, and delay or prevent MIR. This study aimed to explore the role of sleep quality and inflammatory markers on MIRs.

Methods
We conducted a secondary analysis of cross-sectional data of individuals (N=156) having at least one or more MIs within the last 3 to 7 years. Using the hypothalamus-pituitary-adrenal axis model (Irwin, 2019), we tested sleep quality (Pittsburgh Sleep Quality Index [PSQI]) predicting MIR, using inflammatory markers (hs C-Reactor Protein [CRP], Interleukin-1ß [IL-1ß] and Tumor Necrosis Factor alpha [TNFα]) as the simultaneous indirect paths. Race, sex and body mass index (BMI) were also examined using moderated mediation.

Results
The sample ranged in age from 34 to 92 (M = 65.37, SD = 12.13), BMI averaged 31.11 (SD = 7.34), and was comprised of mostly male (57.1%) and White adults (67.9%). PSQI predicted only IL-1ß (ß= .02; p &lt; .01). IL-1ß predicted MIR (ß = .80, p = .05). The direct effect of PSQI to MIR was not significant (p =.12), the indirect path via IL-1ß was. This relationship was not moderated by race, sex, nor BMI.

Conclusion
IL-1ß is an inflammatory marker elevated after acute MI which does not reflect our selected sample. Inflammation may be an important marker of risk for MIR in those with poor sleep quality. Future studies should examine other markers of inflammation and sleep in those with MIR.
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