bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathological Prognostic Factors Influencing Survival Outcomes of Vulvar Cancer
Authors: Muangchang Monwanee , Suprasert Prapaporn , Khunamornpong Surapan 
Published: February 2021

Abstract
Backgroud: Squamous cell carcinoma (SCCA) is the most common vulva cancer. This study purpose to evaluate the clinicopathological prognostic factors for survival outcomes of this disease after treated with surgery. Methods: All SCCA vulva cancer patients who underwent surgery between January 2006 and December 2017 were reviewed. The clinicopathological factors were analyzed to identify the prognostic factors for the progression-free survival (PFS) and overall survival (OS) using the Kaplan- Meier method and Cox-Proportional Hazard model.Results: One hundred twenty-five patients were recruited with a median age of 57 years. The recurrence rate was 35.2%. Patients with recurrence revealed a significant poorer five-year OS rate than those who did not recur (23.7% vs. 79.4%, P < 0.001). About 58.1% of palpable groin nodes revealed metastasis. The independent poor prognostic factors for PFS were groin node-positive and a tumor diameter more than 25 mm. whereas postmenopausal status, preoperative tumor area more than 11 cm2, and groin node enlargement were independent poor prognostic factors for OS. Conclusion: Groin node-positive and tumor diameter longer than 25 mm. were independent poor prognostic factors for PFS whereas postmenopausal status, large tumor area than 11 cm2, and enlargement of groin nodes were independent poor prognostic factors for OS. Patients with these factors should be closely followed.
Identification of a three-gene signature associated with immune infiltration to distinguish follicular thyroid cancer and adenoma: an integrated bioinformatic analysis
Authors: Zhang Yun , Huan WU , Wei XIA , Wei WEI , Huijuan YUAN 
Published: February 2021

Abstract
Background

Follicular thyroid cancer (FTC), accounting for about 15% of all thyroid cancers, was characterized by a more invasive nature and earlier hematogenous spread. The distinction between FTC and follicular thyroid adenoma (FTA) remained challenging for clinical scientists due to their highly similar cytological features at present, and frequently led to potentially unnecessary surgical procedures. The present study aimed to identify potential new biomarkers to improve diagnosis.
Methods

Three GEO datasets (GSE82208, GSE15045, and GSE29315) were downloaded to screen differentially expressed genes (DEGs) between FTC and FTA. GO and KEGG enrichment analysis were explored by ClusterProfiler package. A protein-protein interaction (PPI) network was established and hub genes were selected. Multivariate stepwise logistic regression analysis and receiver operating characteristic (ROC) analysis were used to construct and evaluate a gene signature model for differential diagnosis. Diagnostic efficacy of the model was further confirmed in an independent dataset GSE27155. CIBERSORT was used to characterize 22 infiltrating immune cell fractions of FTC and FTA samples.
Results

After gene integration, a total of 210 DEGs (55 upregulated and 155 downregulated) were identified, which were enriched in immune or cancer-related biological processes and pathways, such as cytokine-cytokine receptor interaction, fluid shear stress and atherosclerosis, and IL-17 signaling pathway. Seven hub genes were selected from PPI analysis. A three-gene signature (FOS, IL1B, and TOP2A) was constructed to distinguish FTC and FTA, with an area under the curve (AUC) of 0.839. Diagnostic efficacy of the signature was further conformed in an independent dataset GSE27155 with an AUC of 0.862. Moreover, the gene signature was associated with CD8 T-cell, which was significant different in FTC compared to FTA.
Conclusions

This study identified potential crucial genes and pathways that might be involved in the pathophysiology of FTC. Especially, a three-gene signature with diagnostic value was development. Furthermore, the landscape of immune infiltration in FTC was explored for the first time. The results would promote our understanding about the molecular mechanism of FTC development and provide potential target genes for differential diagnosis.
Identification and Characterization of the Roles of CircCASP9 in Gastric Cancer Based on a CircRNA–miRNA–mRNA Regulatory Network
Authors: Wang Ziwei , Qin Chuan , Zhang Han , Guo Xiong , Cheng Anqi 
Published: February 2021

Abstract
Background: Circular RNAs (circRNAs) play crucial roles in gastric cancer (GC). This study aimed to construct a new circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network for GC and validate a specific circRNA in GC tissues and cell lines.Methods: We obtained circRNA profiles from an in-house circRNA microarray and the Gene Expression Omnibus (GEO) database, and microRNA (miRNA) profiles from StarBase and CircBank. Messenger RNA profiles were downloaded from GEO. We then constructed a ceRNA network using the extracted circRNA (circCASP9), miRNA (miR-589-5p) and 52 mRNAs. The expression of circCASP9 was confirmed in GC tissues and cell lines, using quantitative reverse transcription polymerase chain reaction. The function of circCASP9 was validated in GC cell lines, using the cell counting kit-8, ethynyl deoxyuridine, transwell, and colon assays. The sponge function of circCASP9 for miR-589-5p was verified using dual luciferase assays. Results: A novel ceRNA network was established with one downregulated circRNA, one upregulated miRNA, and 52 downregulated mRNAs. CircularCASP9 was downregulated in GC tissues and cell lines and sponged miR-589-5p. Furthermore, circCASP9 inhibited GC proliferation, migration, and invasion. Conclusions: We identified the expression and anticancer effects of the core circRNA circCASP9 in a novel circRNA-miRNA-mRNA ceRNA regulatory network of GC. This present study may provide a novel therapeutic target and insight understanding of the mechanism of gastric cancer.
Longitudinal Profiling Of The Burn Patient Cutaneous And Gastrointestinal Microbiota
Authors: Lima Kelly , Davis Ryan , Liu Stephenie , Greenhalgh David , Tran Nam 
Published: February 2021

Abstract
Sepsis is a leading cause of morbidity and mortality in patients that have sustained a severe burn injury. Early detection and treatment of infections improves outcomes and understanding changes in the host microbiome following injury and during treatment may aid in burn care. The loss of functional barriers, systemic inflammation, and commensal community dysbiosis all contribute to a burn patient’s increased risk of infection. We sampled 10 burn patients to evaluate cutaneous microbial populations on the burn wound and corresponding spared skin on days 0, 3, 7, 14, 21, and 28 post-intensive care unit admission. In addition, skin samples were paired with perianal and rectal locations to evaluate changes in the burn patient gut microbiome following injury and treatment. We found significant (P = 0.011) reduction in alpha diversity on the burn wound compared to spared skin throughout the sampling period as well as reduction in common skin commensal bacteria such as Propionibacterium acnes and Staphylococcus epidermitis. Compared to healthy volunteers (n = 18), the burn patient spared skin also exhibited a significant reduction in diversity (P = 0.001). Treatments such as systemic or topical antibiotic administration, skin grafting, and nutritional formulations also impact diversity and community composition at the sampling locations. When evaluating each subject individually, an increase in relative abundance of a clinically isolated bacteria could be seen in 5/9 infections detected among the burn patient cohort.
The Different Outcomes Between Breast-Conserving Surgery plus Radiotherapy and Mastectomy in Metaplastic Breast Cancer: A Population-Based Study
Authors: Xia Lin-Yu , Xu Wei-Yun , Hu Qing-Lin 
Published: February 2021

Abstract
Background: Metaplastic breast cancer (MBC) are rare. The survival outcomes of MBC patients after breast conserving surgery plus radiotherapy (BCS+RT) or mastectomy have not been established. The aim of this study was to compare survival outcomes of MBC patients subjected to BCS+RT or mastectomy therapeutic options.Methods: Patients who were subjected to BCS+RT or mastectomy between 2004 and 2014 were enrolled in this study through the Surveillance, Epidemiology and End Results (SEER) database. Breast cancer-specific survival (BCSS) and the overall survival (OS) of the participants were determined. Cox proportional hazard model and Kaplan Meier method were used to determine the correlation between the two surgical methods and survival outcomes. Results: A total of 1197 patients were enrolled in this study. Among them, 439 patients were subjected to BCS+RT, while 758 patients were subjected to mastectomy. Multivariate analysis showed a significantly high OS for MBC patients who were subjected to BCS+RT compared to those subjected to mastectomy (HR = 0.697, 95% CI = 0.527- 0.922, P=0.012). However, the BCSS for the two groups were statistically comparable (HR = 0.806,95% CI = 0.561-1.158, P= 0.244). After PSM, the BCS+RT and mastectomy groups consisted of 321 patients, respectively. The univariate and multivariate analysis with a 6-month landmark all indicate that patients receiving BCS+RT has higher OS than patients receiving mastectomy (HR = 0.701,95% CI = 0.496-0.990, P=0.044; HR = 0.684,95% CI = 0.479-0.977, P=0.037) while the BCSS was no difference between the two groups(HR = 0.739,95% CI = 0.474-1.153, P = 0.183; HR = 0.741,95% CI = 0.468-1.173, P = 0.200). Conclusion: The BCS+RT therapeutic option exhibits a high OS in MBC patients compared to the mastectomy approach.
Manipulating electron redistribution to achieve exotic electronic pyroelectricity in dynamic [FeCo] crystals
Authors: Sadhukhan Pritam , Wu Shu-Qi , Long Jeremy , Nakanishi Takumi , Kanegawa Shinji , Gao Kaige , Yamamoto Kaoru , Okajima Hajime , Sakamoto Akira , Baker Michael , Kroll Thomas , Sokaras Dimosthenis , Okazawa Atsushi , Kojima Norimichi , Shiota Yoshihito , Yoshizawa Kazunari , Sato Osamu 
Published: February 2021

Abstract
Pyroelectricity plays a crucial role in modern sensors and energy conversion devices. However, obtaining materials with large and nearly constant pyroelectric coefficients over a wide temperature range for the practical uses remains a formidable challenge, because in conventional ferroelectric materials the pyroelectric effect promptly declines upon cooling from the transition temperature. Attempting to discover a solution to this obstacle, we combined molecular design of labile electronic structure with the crystal engineering of the molecular orientation in lattice resulting in the electronic pyroelectricity of purely molecular origin. Here, we report a polar crystal of an [FeCo] dinuclear complex exhibiting a peculiar pyroelectric behavior (a substantial sharp pyroelectric current peak and an unusual continuous pyroelectric current at higher temperatures) which is caused by a combination of Fe spin transition and redistribution of electron density between redox isomers of high-spin Fe through a charge transfer between the Fe atom and redox active ligand. As a result, temperature dependence of the pyroelectric behavior reported here is opposite and originates from the interconversion between three distinct electronic states. The obtained pyroelectric coefficient is comparable to that of polyvinylidene difluoride at room temperature.
Phosphorus coordinated Rh single-atom sites on nanodiamond as highly regioselective catalyst for hydroformylation of olefins
Authors: Gao Peng , Liang Guanfeng , Ru Tong , Liu Xiao Yan , Qi Haifeng , Wang Aiqin , Chen Fener 
Published: February 2021

Abstract
Single-atom Rh catalysts present superior activity relative to homogeneous catalyst in olefins hydroformylation, yet with limited success in regioselectivity control. In the present work, we develop a novel phosphorus coordinated Rh1 single-atom catalyst with nanodiamond as support. Benefiting from this unique structure, the catalyst exhibits excellent activity and regioselectivity for the hydroformylation of arylethylenes with wide substrate generality, i.e., with high conversion (>99%) and high regioselectivity (>90%), which is comparable with the homogeneous counterparts. The coordination interaction between Rh1 and surface phosphorus species is clarified by 31P solid-state NMR and X-ray absorption spectroscopy (XAS). Rh single atoms are firmly anchored over nanodiamond through Rh-P bonds, guaranteeing good stability in the hydroformation of styrene even after six runs. Finally, by using this new catalyst, two kinds of pharmaceutical molecules, Ibuprofen and Fendiline, are synthesized efficiently with high yields, demonstrating a new prospect of single-atom catalyst in pharmaceutical synthesis.
Photoresponse properties of Au/(CoFe2O4-PVP)/n-Si/Au (MPS) diode
Authors: Ulusan A. Buyukbas , Tataroglu Adem , altindal S. , Azizian-Kalandaragh Y. 
Published: February 2021

Abstract
Photo-response properties of the Au/(CoFe2O4-PVP)/n-Si (MPS) diode were investigated using current-voltage (I-V) measurements achieved under dark and various illumination conditions. The experimental results showed that the MPS diode has a good response to the illumination. Especially, in reverse-bias region, photocurrent (Iph) increases with increasing illumination intensity (P) due to the formation of electron–hole pairs. The double-logarithmic Iph-P plot has a good relation with 1.27 slope and such high value of slope indicates a lower density of the unoccupied trap level. This indicates that the diode exhibits a good photoconductive and photovoltaic behavior. The photo-to-dark current ratio confirms the photo-sensitivity of the diode. Thermionic emission (TE) theory was used to determine the diode electronic parameters such as saturation current (I0), ideality factor (n) and barrier height (ΦB0) and their values were calculated from the measured I-V data. Moreover, the ΦB0 and series resistance (Rs) were extracted from an alternative method suggested by Norde. All these parameters (ΦB0, n, Rs, and I0) decrease with increasing illumination intensity and there is a good linear correlation between ΦB0 and n as ΦB0 (n) = 4.72x10− 2n + 0.5464 eV. As a results, the fabricated MPS diode due to the excellent photo-response can be used for photovoltaic applications.
The Impact of The [TIMP-2] × [IGFBP7]-Guided Checklist for Early Recognition and Treatment of Acute Illness on The Prevention of Acute Kidney Injury In Patients With Septic Shock
Authors: Wang Jingjing , Li Jing , Wang Dongqiang , Wang Yongqiang , Dou Lin , Gao Hongmei 
Published: February 2021

Abstract
Background: Sepsis is the main aetiology of acute kidney injury (AKI) in critically ill patients, with high morbidity and mortality. The early identification of septic patients at high risk for AKI, followed by the timely implementation of appropriate interventions, is crucial for improving patient outcomes. Tissue inhibitor of metalloproteinase-2 and insulin-like growth factor-binding protein 7 ([TIMP-2] × [IGFBP7]) are promising biomarkers for AKI. Furthermore, the Checklist for Early Recognition and Treatment of Acute Illness (CERTAIN) is a tool for evaluating and treating acute illness in a timely manner based on best practices. We hypothesized that the use of this biomarker-guided point-of-care tool would improve the prognosis of patients with sepsis-associated acute kidney injury (SA-AKI).Methods: This was a single-centre prospective before-and-after study in the ICU of a comprehensive tertiary hospital. From June 2015 to December 2020, we assessed and managed patients with septic shock based on the biomarker-guided CERTAIN checklist. A checklist based on the Sepsis 3.0 bundle and urinary [TIMP-2] × [IGFBP7]>0.3(ng/mL)2/1000 within 12 hours were used as the inclusion criteria. The CERTAIN checklist includes the care bundles recommended in the Sepsis 3.0 bundle and Kidney Disease: Improving Global Outcomes (KDIGO) 2012 guidelines. The primary outcome was the incidence of moderate-to-severe AKI on the first day and within seven days after enrolment and mortality at 90 days after discharge from the hospital. The secondary outcomes were the ICU length of stay, non-mechanical ventilation duration, and the proportions of patients with the recovery of renal function and major adverse kidney events (MAKEs) at 90 days after exposure to events initiating AKI.Results: At the end of the study, 124 patients had been treated based on the checklist. When compared to a cohort of 112 patients matched for disease, the CERTAIN group had a lower proportion of patients needing mechanical ventilation, a lower proportion of patients needing vasoactive agents, a shorter ICU length of stay, and a higher proportion of patients with recovered kidney function. Furthermore, there was a trend towards a higher 90-day survival rate in the CERTAIN group.Conclusions: Implementation of the CERTAIN checklist was associated with improvements in the short-term recovery of kidney function, airway and haemodynamic management and mortality in patients with SA-AKI.Trial registration: NCT01973829; Date of registration: November 1, 2013.
Effect of Cu&nbsp;Substitution on Magnetic and DC&nbsp;Electrical Resistivity Properties of Ni-Zn&nbsp;Nano Ferrites
Authors: Chandramouli K. , Rao P. Anantha , Suryanarayana B. , Raghavendra Vemuri , Parajuli D. , Taddesse Paulos , Mammo Tulu Wegayehu , Raju M. K. , Nandigam Murali 
Published: February 2021

Abstract
Cu substituted Ni0.5Zn0.5-xCuxFe2O4 (x = 0, 0.1, 0.2, 0.3 and 0.4) samples is synthesized using the sol-gel auto-combustion process. X-ray diffraction shows its cubic spinel structure. The lattice constant decreases as the Cu content increases. The sizes of the crystallites are also decreasing in the range of 42.68 nm to 21.75 nm. The wavenumbers of tetrahedral and octahedral sites sighted in the FTIR spectra are similar to that of the precursor. The increment in the copper content increases the DC conductivity. The electrical resistivity decrease with increase in the temperature, i.e. it has a negative temperature coefficient with resistance similar to semiconductors. The remnant ratios R obtained from VSM show their isotropic nature forming single domain ferrimagnetic particles. The resultant material is widely significant, as indicated by its result.
Evaluation of COVID-19 Pandemic in Six Asia-Pacific Countries using Data-Driven and Machine Learning Method Based on the Current Management and Interventions
Authors: Ao Shuang , Huang Wenbo , Han Dan , Liu Yuming , Liu Shuang , huang yaojiang 
Published: February 2021

Abstract
Background South-east Asia and Western Pacific countries have large populations and underreporting of Covid19, which pose challenges to the large-scale response. Methods Data-driven methods are used to evaluate the Government or society’s interventions and the situation of the COVID-19 pandemic, and machine learning method are used to forecast the trend of COVID-19 pandemic based on the current management and interventions. Results The results show that. India received low government response index scores in February, and the number of confirmed cases and active cases in September became quite high with large stock and the overall growth rate is higher than 1. The number of daily confirmed cases in Bangladesh, Japan and Philippines is low and on the decline, it is rising in Malaysia and Indonesia. The number of active cases in Bangladesh, Japan, India and Bangladesh has begun to decline, Malaysia and Indonesia is no sign of decline. Bangladesh, Japan and Philippines will be flat or moderating, while Malaysia and Indonesia will still have no slowdown momentum and the situation will be severe. Conclusions The results show that the existing management and interventions responses are effective, although they have room for improvement, and Malaysia and Indonesia need to be improved and strengthened.
Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo
Authors: Cai Lihui , Gao Nan , Sun Tingyu , Bi Ke , Chen Xin , Zhao Xia 
Published: February 2021

Abstract
Background: Research on the degradation of silk fibroin (SF) scaffolds in vivo lacks uniform and effective standards and experimental evaluation methods. This study aims to evaluate the application of ultrasound in assessing the degradation of SF scaffolds.Methods: Two groups of three-dimensional regenerated SF scaffolds (3D RSFs) were implanted subcutaneously into the backs of Sprague-Dawley rats. B-mode ultrasound and hematoxylin and eosin (HE) staining were performed on days 3, 7, 14, 28, 56, 84, 112, 140, and 196. The cross-sectional areas for two groups of 3D RSFs that were obtained using these methods were semi-quantitatively analyzed and compared to evaluate the biodegradation of the implanted RSFs.Results: Semi-quantitative analysis of the cross-sectional areas detected using B-mode ultrasound revealed that the degradations of the two 3D RSF groups were significantly different. The degradation rate of the SF-B group was found to be higher than that of the SF-A group. This was consistent with the semi-quantitative detection results for HE staining. Regression analysis showed that the results of the B-mode ultrasound and HE staining were correlated in both groups, indicating that B-mode ultrasound is a reliable method to evaluate the SF scaffold degradation in vivo. As the SF scaffold degraded, its echo gradually decreased. In the early stages of degradation, the echo of the SF scaffold was higher than that of the muscle. In the middle stage of degradation, the echo was equal to that of the muscle. In the later stage, the echo was lower than that of the muscle.Conclusions: This study suggests that B-mode ultrasound can clearly display the implanted SF scaffolds non-invasively and monitor the degradation of the different SF scaffolds after implantation in living organisms in real-time.
First-line pembrolizumab, trastuzumab, and chemotherapy in advanced HER2-positive gastric cancer with sequential genomic profiling
Authors: Chung Hyun Cheol , Lee Choong-kun , Rha Sun Young , Kim Hyo Song , Jung Minkyu , Kang Beodeul , Che Jingmin , Kwon Woo Sun , Bae Woo Kyun , Koo Dong-Hoe , Shin Su-Jin , Kim Hyunki , Jeung Hei-Cheul , Zang Dae Young , Lee Sang Kil , Nam Chung Mo 
Published: February 2021

Abstract
Combining programmed cell death 1 (PD-1) and human epidermal receptor 2 (HER2) targeting agents has shown synergy in HER2-positive preclinical cancer models. Here, we describe a single-arm multi-institutional phase Ib/II trial combining pembrolizumab, trastuzumab, and chemotherapy (capecitabine plus cisplatin) as first-line therapy for HER2-positive advanced gastric cancer (AGC). With a median follow-up of 18.2 months, 3 out of 43 enrolled patients remained in the treatment and 7 finished 2-year treatment without progression. Objective response rate was 76.7% (complete response 16.3%, partial response 60.5%, conversion surgery 4.6%), with 26 patients (56.6%) showing tumor reduction of more than 50%. Median progression-free survival was 8.6 months (95% confidence interval [CI] 7.2–16.4) and median overall survival was 19.3 months (95% CI 16.5–not reached). There was no patient with microsatellite instability-high or Epstein–Barr virus-positive tumor. No patient discontinued pembrolizumab because of immune-related adverse events. Programmed death ligand-1 (PD-L1) status, metastatic organ, or baseline tumor burden were not related to survival. Molecular analyses of pre-treatment tumor samples using next-generation panel sequencing (NGS) showed that HER2 amplification, RTK/RAS pathway alteration, and neoantigen load corrected by HLA-B were related to survival. Comparison analyses of sequentially biopsied samples identified sensitive and resistant sub-clones with spatial and longitudinal tumor heterogeneity. This study provided insight into potentially relevant NGS-based genomic changes to identify patients who may benefit from quadruplet regimen (pembrolizumab, trastuzumab, capecitabine, and cisplatin), which was active and safe for HER2-positive AGC.