bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional anatomy of the Denonvilliers’ fascia after micro-CT reconstruction
Authors: Yang Seung Yoon , Kim Ho Seung , Cho Min Soo , Kim Nam Kyu 
Published: November 2021

AbstractAn understanding of the anatomy of the Denonvilliers’ fascia is essential for successful surgical outcomes for patients with rectal cancer in the mid- to lower regions, especially near the seminal vesicles and prostate in males. Whether the correct surgical plane during a total mesorectal excision should be anterior or posterior to the Denonvilliers’ fascia is currently under debate. This study aimed to investigate the Denonvilliers’ fascia using micro-computed tomography (micro-CT) to acquire three-dimensional images nondestructively for assessments of the relationship between the Denonvilliers’ fascia, the mesorectal fascia, and neurovascular bundles to elucidate the correct anterior total mesorectal excision plane. Eight specimens were obtained bilaterally from four fresh human cadavers. Four specimens were stained with phosphotungstic acid to visualize the soft tissue, and micro-CT images were obtained; the other four specimens were stained with Masson’s trichrome to visualize connective tissue. Micro-CT images corroborate that the Denonvilliers’ fascia consists of a multilayered structure that separates the rectum from the seminal vesicles and the prostate. Specimens stained with Masson’s trichrome showed that the urogenital neurovascular bundle located at the posterolateral corner of the prostate is separated from the mesorectum by the Denonvilliers’ fascia. For the preservation of autonomic nerves necessary for urogenital function and optimal oncologic outcomes in patients with rectal cancer, a successful mesorectal excision requires a dissection plane posterior to the Denonvilliers’ fascia.
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
Authors: Kubota Masaaki , Yoshida Yoichi , Kobayashi Eiichi , Matsutani Tomoo , Li Shu-Yang , Zhang Bo-Shi , Mine Seiichiro , Machida Toshio , Takizawa Hirotaka , Hiwasa Takaki , Iwadate Yasuo 
Published: November 2021

AbstractThe presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction.
Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion
Authors: Poeta Marco , Cioffi Valentina , Buccigrossi Vittoria , Nanayakkara Merlin , Baggieri Melissa , Peltrini Roberto , Amoresano Angela , Magurano Fabio , Guarino Alfredo 
Published: November 2021

AbstractSARS-CoV-2 enters the intestine by the spike protein binding to angiotensin-converting enzyme 2 (ACE2) receptors in enterocyte apical membranes, leading to diarrhea in some patients. Early treatment of COVID-19-associated diarrhea could relieve symptoms and limit viral spread within the gastrointestinal (GI) tract. Diosmectite, an aluminomagnesium silicate adsorbent clay with antidiarrheal effects, is recommended in some COVID-19 management protocols. In rotavirus models, diosmectite prevents pathogenic effects by binding the virus and its enterotoxin. We tested the trapping and anti-inflammatory properties of diosmectite in a SARS-CoV-2 model. Trapping effects were tested in Caco-2 cells using spike protein receptor-binding domain (RBD) and heat-inactivated SARS-CoV-2 preparations. Trapping was assessed by immunofluorescence, alone or in the presence of cells. The effect of diosmectite on nuclear factor kappa B (NF-kappaB) activation and CXCL10 secretion induced by the spike protein RBD and heat-inactivated SARS-CoV-2 were analyzed by Western blot and ELISA, respectively. Diosmectite bound the spike protein RBD and SARS-CoV-2 preparation, and inhibited interaction of the spike protein RBD with ACE2 receptors on the Caco-2 cell surface. Diosmectite exposure also inhibited NF-kappaB activation and CXCL10 secretion. These data provide direct evidence that diosmectite can bind SARS-CoV-2 components and inhibit downstream inflammation, supporting a mechanistic rationale for consideration of diosmectite as a management option for COVID-19-associated diarrhea.
A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein
Authors: Wolfe Monica , Webb Sean , Chushak Yaroslav , Krabacher Rachel , Liu Yi , Swami Nathan , Harbaugh Svetlana , Chávez Jorge 
Published: November 2021

AbstractRapid design, screening, and characterization of biorecognition elements (BREs) is essential for the development of diagnostic tests and antiviral therapeutics needed to combat the spread of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address this need, we developed a high-throughput pipeline combining in silico design of a peptide library specific for SARS-CoV-2 spike (S) protein and microarray screening to identify binding sequences. Our optimized microarray platform allowed the simultaneous screening of ~ 2.5 k peptides and rapid identification of binding sequences resulting in selection of four peptides with nanomolar affinity to the SARS-CoV-2 S protein. Finally, we demonstrated the successful integration of one of the top peptides into an electrochemical sensor with a clinically relevant limit of detection for S protein in spiked saliva. Our results demonstrate the utility of this novel pipeline for the selection of peptide BREs in response to the SARS-CoV-2 pandemic, and the broader application of such a platform in response to future viral threats.
Narrow-band random Raman lasing from Rhodamine 6G assisted by cascaded stimulated Raman scattering effect
Authors: Hosseini Mandana Sadat , Yazdani Elnaz , Sajad Batool 
Published: November 2021

AbstractThis study reports the first experimental observation of cascaded stimulated Raman scattering (SRS) generation in a colloidal disordered medium. Generation of the cascaded effect requires both a high Raman gain and pump power in the disordered medium. Here, to extend effective path lengths of photons into the Raman gain medium for producing additional SRS processes, ZnO microspheres with abundant nano-protrusions as suitable scattering centers are proposed. It is explained that nano-protrusions on the surface of the spheres can act as nano reflectors and significantly provide potent feedback in the disordered system. This provided feedback via nano-protrusions boosts cascaded SRS generation to allow the appearance of higher Raman signals of Rhodamine 6G dye solution at a low scatterer concentration of 5 mg/ml. The threshold for the formation of the first Raman signal is measured at about 60 mJ/pulse. Also, the evolution of Raman signals under several fixed pump pulses is examined to investigate the stability from pulse to pulse. Our findings provide promising perspectives for achieving the single-frequency laser sources and generate desirable wavelengths for specific applications.
A traumatic injury mortality prediction (TRIMP) based on a comprehensive assessment of abbreviated injury scale 2005 predot codes
Authors: Wang Muding , Zhang Guohu , Cong Degang , Zeng Yunji , Fan Wenhui , Shen Yi 
Published: November 2021

AbstractAbbreviated Injury Scale (AIS)-based systems such as injury severity score (ISS), exponential injury severity score (EISS), trauma mortality prediction model (TMPM), and injury mortality prediction (IMP), classify anatomical injuries with limited accuracy. The widely accepted alternative, trauma and injury severity score (TRISS), improves the prediction rate by combining an anatomical index of ISS, physiological index (the Revised Trauma Score, RTS), and the age of patients. The study introduced the traumatic injury mortality prediction (TRIMP) with the inclusion of extra clinical information and aimed to compare the ability against the TRISS as predictors of survival. The hypothesis was that TRIMP would outperform TRISS in prediction power by incorporating clinically available data. This was a retrospective cohort study where a total of 1,198,885 injured patients hospitalized between 2012 and 2014 were subset from the National Trauma Data Bank (NTDB) in the United States. A TRIMP model was computed that uses AIS 2005 (AIS_05), physiological reserve and physiological response indicators. The results were analysed by examining the area under the receiver operating characteristic curve (AUC), the Hosmer–Lemeshow (HL) statistic, and the Akaike information criterion. TRIMP gave both significantly better discrimination (AUCTRIMP, 0.964; 95% confidence interval (CI), 0.962 to 0.966 and AUCTRISS, 0.923; 95% CI, 0.919 to 0.926) and calibration (HLTRIMP, 14.0; 95% CI, 7.7 to 18.8 and HLTRISS, 411; 95% CI, 332 to 492) than TRISS. Similar results were found in statistical comparisons among different body regions. TRIMP was superior to TRISS in terms of accurate of mortality prediction, TRIMP is a new and feasible scoring method in trauma research and should replace the TRISS.
Teaching and Learning in the School Chemistry Laboratory
Authors: Hofstein Avi , Hugerat Muhamad 
Published: November 2021

Research into the educational effectiveness of chemistry practical work has shown that the laboratory offers a unique mode of instruction, assessment and evaluation. Laboratory work is an integral and important part of the learning process, used to encourage the development of high order thinking and learning alongside high order learning and thinking skills such as argumentation and metacognition.
Authored by renowned experts in the field of chemistry education, this book provides a holistic approach to cover all issues related to learning and teaching in the chemistry laboratory. With sections focused on developing the skill sets of teachers, as well as approaches to supporting students in the laboratory, the book offers a comprehensive look at vicarious instruction methods, teacher and students' roles, and the blend with ICT, simulations, and other effective approaches to practical work. The book concludes with a focus on retrospective issues, followed-up with a look to the future of laboratory learning.
A product of nearly fifty years of research, this book will be useful for chemistry teachers, curriculum developers, researchers in chemistry education, and professional development providers.
The HD 137496 system: A dense, hot super-Mercury and a cold Jupiter
Authors: Azevedo Silva T. , Demangeon O. D. S. , Barros S. C. C. , Armstrong D. J. , Otegi J. F. , Bossini D. , Delgado Mena E. , Sousa S. G. , Adibekyan V. , Nielsen L. D. , Dorn C. , Lillo-Box J. , Santos N. C. , Hoyer S. , Stassun K. G. , Almenara J. M. , Bayliss D. , Barrado D. , Boisse I. , Brown D. J. A. , Díaz R. F. , Dumusque X. , Figueira P. , Hadjigeorghiou A. , Hojjatpanah S. , Mousis O. , Osborn A. , Santerne A. , Strøm P. A. , Udry S. , Wheatley P. J. 
Published: November 2021

Context. Most of the currently known planets are small worlds with radii between that of the Earth and that of Neptune. The characterization of planets in this regime shows a large diversity in compositions and system architectures, with distributions hinting at a multitude of formation and evolution scenarios. However, many planetary populations, such as high-density planets, are significantly under-sampled, limiting our understanding of planet formation and evolution.
Aims. NCORES is a large observing program conducted on the HARPS high-resolution spectrograph that aims to confirm the planetary status and to measure the masses of small transiting planetary candidates detected by transit photometry surveys in order to constrain their internal composition.
Methods. Using photometry from the K2 satellite and radial velocities measured with the HARPS and CORALIE spectrographs, we searched for planets around the bright (Vmag = 10) and slightly evolved Sun-like star HD 137496.
Results. We precisely estimated the stellar parameters, M* = 1.035 ± 0.022 M⊙, R* = 1.587 ± 0.028 R⊙, Teff = 5799 ± 61 K, together with the chemical composition (e.g. [Fe/H] = −0.027 ± 0.040 dex) of the slightly evolved star. We detect two planets orbiting HD 137496. The inner planet, HD 137496 b, is a super-Mercury (an Earth-sized planet with the density of Mercury) with a mass of Mb = 4.04 ± 0.55 M⊕, a radius of Rb = 1.31−0.05+0.06 R⊕, and a density of ρb = 10.49−1.82+2.08 g cm-3. With an interior modeling analysis, we find that the planet is composed mainly of iron, with the core representing over 70% of the planet’s mass (Mcore / Mtotal = 0.73−0.12+0.11). The outer planet, HD 137496 c, is an eccentric (e = 0.477 ± 0.004), long period (P = 479.9−1.1+1.0 days) giant planet (Mc sinic = 7.66 ± 0.11 MJup) for which we do not detect a transit.
Conclusions. HD 137496 b is one of the few super-Mercuries detected to date. The accurate characterization reported here enhances its role as a key target to better understand the formation and evolution of planetary systems. The detection of an eccentric long period giant companion also reinforces the link between the presence of small transiting inner planets and long period gas giants.
A polarimetric study of asteroids in comet-like orbits
Authors: Geem Jooyeon , Ishiguro Masateru , Bach Yoonsoo P. , Kuroda Daisuke , Naito Hiroyuki , Hanayama Hidekazu , Kim Yoonyoung , Kwon Yuna G. , Jin Sunho , Sekiguchi Tomohiko , Okazaki Ryo , Vaubaillon Jeremie J. , Imai Masataka , Oono Tatsuharu , Futamura Yuki , Takagi Seiko , Sato Mitsuteru , Kuramoto Kiyoshi , Watanabe Makoto 
Published: November 2021

Context. Asteroids in comet-like orbits (ACOs) consist of asteroids and dormant comets. Due to their similar appearance, it is challenging to distinguish dormant comets from ACOs via general telescopic observations. Surveys for discriminating dormant comets from the ACO population have been conducted via spectroscopy or optical and mid-infrared photometry. However, they have not been conducted through polarimetry.
Aims. We conducted the first polarimetric research of ACOs.
Methods. We conducted a linear polarimetric pilot survey for three ACOs: (944) Hidalgo, (3552) Don Quixote, and (331471) 1984 QY1. These objects are unambiguously classified into ACOs in terms of their orbital elements (i.e., the Tisserand parameters with respect to Jupiter TJ significantly less than 3). Three ACOs were observed by the 1.6 m Pirka Telescope from UT 2016 May 25 to UT 2019 July 22 (13 nights).
Results. We found that Don Quixote and Hidalgo have polarimetric properties similar to comet nuclei and D-type asteroids (optical analogs of comet nuclei). However, 1984 QY1 exhibited a polarimetric property consistent with S-type asteroids. We conducted a backward orbital integration to determine the origin of 1984 QY1, and found that this object was transported from the main belt into the current comet-like orbit via the 3:1 mean motion resonance with Jupiter.
Conclusions. We conclude that the origins of ACOs can be more reliably identified by adding polarimetric data to the color and spectral information. This study would be valuable for investigating how the ice-bearing small bodies distribute in the inner Solar System.
The Development of Local Culture as a Model for the Development of Maritime Tourism: A Study on Ritual Sedekah Laut in Gempolsewu Weleri
Authors: Ken Widyatwati , Mahfudz  
Published: November 2021

The Sedekah Laut in Gempolsewu ritual is a traditional ritual of fishermen in Weleri which is held every year. Philosophically,	Sedekah Laut is an activity as a form of gratitude to God for the gifts and salvation that has been given. The Weleri Kendal area is included in the Regional Tourism Corridor development plan aimed at developing maritime tourism. This paper focuses on maritime tourism modeling for creative sustainable regional development by developing a unique identity or characteristic in the Weleri area with the aim of increasing community empowerment and income. This article describes the basic concept of developing local culture as a form of maritime tourism development, explaining the relationship between the maritime tourism product model and the development of local culture in the context of empowerment and increasing community income. The basic for the development of local culture includes: Publication or introducing Gempolsewu as a maritime area in Central Java. Developing the diversity of cultural arts of the Gempolsewu community in the form of cultural attractions and making collaborations between cultural attractions and the availability of distinctive culinary delights. Advancing the folk handicraft industry to support the development of maritime tourism areas in Gempolsewu.
Nano-polycrystalline Ag-doped ZnO layer for steep-slope threshold switching selectors
Authors: Sahota Akshay , Kim Harrison Sejoon , Mohan Jaidah , Le Dan N. , Jung Yong Chan , Kim Si Joon , Lee Jang-Sik , Ahn Jinho , Hernandez-Arriaga Heber , Kim Jiyoung 
Published: November 2021

In this work, a nano-polycrystalline Ag-doped ZnO-based threshold switching (TS) selector via a facile co-sputtering technique is investigated without using an Ag active metal layer. The effects of the Ag concentration with respect to OFF-state leakage current (Ioff) were studied, and the results demonstrate that by regulating the Ag doping concentration in the switching layer (SL), an electroforming-free switching with an Ion/Ioff ratio of ∼108 could be achieved, having an extremely low Ioff value of ∼10−13 A. Furthermore, cycling endurance can also be improved as the formation of a laterally thick and stable filament does not happen promptly with consequent measurements when there is a limited amount of Ag in the SL. The selector device performance enhancement is attributed to the doping-based polycrystalline structure that facilitates enhanced control on filament formation due to the restricted availability and anisotropic diffusion of Ag ions in the polycrystalline ZnO SL, thereby trimming down the overall stochasticity during metallic filament growth. The present study demonstrates that a doping-based polycrystalline SL structure can be implemented in a selector device to augment TS characteristics, i.e., device variances and cycling endurance for adoption in ultra-high density memory applications.
The role of melatonin implantation and the associated effect of insulin-like growth factor-1 on milk composition of Barki ewes
Authors: El-Mokadem M. Y. , Nour El-Din A. N. M. , Ramadan T. A. , Taha T. A. , Samak M. A. , Sharaby M. A. , Salem M. H. , Rashad A. M. 
Published: November 2021

Context Manipulation of milk composition in lactating ewes represents an important mechanism by which sheep breeders can improve profits through increased milk prices for cheese making and higher lamb growth rates. To this end, melatonin implantation may affect milk composition by alleviating reproductive seasonality and augmenting insulin like growth factor-1 (IGF-1). Aims This research aimed to evaluate the effects of melatonin administration on hormone levels and milk composition, and to determine its potential to manipulate milk composition of lactating Barki ewes. Methods Fifty lactating Barki ewes were divided into two groups of 25. One group received 18 mg melatonin in a single implant at 40 days after delivery, and the other group served as an untreated control. Over the following 8 weeks, milk components (fat, protein, lactose and total solids percentages), milk energy value, and blood serum hormones including IGF-1 were measured. Key results Melatonin implantation increased (P < 0.05) IGF-1 concentration, which showed continued elevation (P < 0.05) throughout the treatment period (from Day 14 to Day 56) relative to the control. Analysis of milk composition of implanted ewes revealed higher (P < 0.05) milk fat, protein, lactose and total solids percentages as well as milk energy value than in milk of control ewes. Conclusion An integrated effect of melatonin and IGF-1 successfully improved milk composition of lactating Barki ewes. Implications Melatonin implantation can be used to improve milk composition of lactating Barki ewes, potentially increasing profits from cheese making and lamb production.
Effects of gypsum on growth and nutrient status of forage grasses cultivated between the rows of organically grown Satsuma mandarin in an Oxisol from subtropical Brazil
Authors: Korzune Maciel , Ávila Fabrício William , Botelho Renato Vasconcelos , Muller Marcelo Marques Lopes , Petranski Poliana Horst , Pinto Eduardo Luiz Costa Tobias , Aksenen Talia , Jadoski Sidnei Osmar , Rampim Leandro 
Published: November 2021

Gypsum (CaSO4.2H2O) decreases the harmful effect of soil acidity on plants and provides calcium (Ca) and sulfur (S) for plant nutrition. Setaria grass (Setaria anceps Stapf) and black oat (Avena strigosa Schreb.) are forage grasses that are tolerant of low soil fertility and may be suitable as inter-row crops for Satsuma mandarin (Citrus unshiu Marc.) grown organically on Oxisol soils, which may suffer low native fertility and high acidity. We evaluated the effect of gypsum application to a low fertility soil from subtropical Brazil on soil chemical fertility, growth and plant nutrition of Satsuma mandarin, and plant biomass yield and soil nutrient extraction of setaria grass and black oat cultivated between the orchard rows, in the absence of soluble industrial (NPK) fertilisers. The entire experimental field was limed, and ground natural phosphate rock was applied. During planting of the Satsuma mandarin, organic manure and ground natural phosphate rock were mixed with soil and placed in each planting hole. Two agricultural gypsum treatments were applied: one without gypsum (control), and the other with gypsum at 6 t ha−1. After 12 months, gypsum reduced the level of aluminium (Al3+) and increased Ca2+ at soil depth 0–40 cm, and increased S-SO42− at soil depth 0–60 cm. Other soil chemical components (pH, phosphorus, potassium, magnesium and micronutrients) were not affected by gypsum application. Gypsum did not affect growth of Satsuma mandarin, but increased leaf S concentration. Between the rows, gypsum decreased nitrogen extraction by setaria grass and increased Ca and S extractions by black oat. Biomass yield and nutrient extraction by the studied forage grasses were satisfactory, considering the poor soil fertility conditions. Setaria grass exhibited higher efficiency in recycling nutrients from the chemically poor soils than black oat. In general, setaria grass and black oat can be used as inter-row forage grasses for organic orchards of Satsuma mandarin, where there are restrictions on use of soluble industrial fertilisers.