bluuebunny/crossref_metadata_2025_split huggingface dataset:
The phycobilisome core‐membrane linkers from Synechocystis sp. PCC 6803 and red‐algae assemble in the same topology
Authors: Niu Nan‐Nan , Lu Lu , Peng Pan‐Pan , Fu Zhi‐Juan , Miao Dan , Zhou Ming , Noy Dror , Zhao Kai‐Hong 
Published: June 2021

SUMMARYThe phycobilisomes (PBSs) of cyanobacteria and red‐algae are unique megadaltons light‐harvesting protein‐pigment complexes that utilize bilin derivatives for light absorption and energy transfer. Recently, the high‐resolution molecular structures of red‐algal PBSs revealed how the multi‐domain core‐membrane linker (LCM) specifically organizes the allophycocyanin subunits in the PBS’s core. But, the topology of LCM in these structures was different than that suggested for cyanobacterial PBSs based on lower‐resolution structures. Particularly, the model for cyanobacteria assumed that the Arm2 domain of LCM connects the two basal allophycocyanin cylinders, whereas the red‐algal PBS structures revealed that Arm2 is partly buried in the core of one basal cylinder and connects it to the top cylinder. Here, we show by biochemical analysis of mutations in the apcE gene that encodes LCM, that the cyanobacterial and red‐algal LCM topologies are actually the same. We found that removing the top cylinder linker domain in LCM splits the PBS core longitudinally into two separate basal cylinders. Deleting either all or part of the helix‐loop‐helix domain at the N‐terminal end of Arm2, disassembled the basal cylinders and resulted in degradation of the part containing the terminal emitter, ApcD. Deleting the following 30 amino‐acids loop severely affected the assembly of the basal cylinders, but further deletion of the amino‐acids at the C‐terminal half of Arm2 had only minor effects on this assembly. Altogether, the biochemical data are consistent with the red‐algal LCM topology, suggesting that the PBS cores in cyanobacteria and red‐algae assemble in the same way.
Pericentromeric chromatin reorganisation follows the initiation of recombination and coincides with early events of synapsis in cereals
Authors: Lenykó‐Thegze Andrea , Fábián Attila , Mihók Edit , Makai Diána , Cseh András , Sepsi Adél 
Published: June 2021

SummaryThe reciprocal exchange of genetic information between homologous chromosomes during meiotic recombination is essential to secure balanced chromosome segregation and to promote genetic diversity. The chromosomal position and frequency of reciprocal genetic exchange shapes the efficiency of breeding programmes and influences crop improvement under a changing climate. In large genome cereals, such as wheat and barley, crossovers are consistently restricted to subtelomeric chromosomal regions, thus preventing favourable allele combinations being formed within a considerable proportion of the genome, including interstitial and pericentromeric chromatin. Understanding the key elements driving crossover designation is therefore essential to broaden the regions available for crossovers. Here, we followed early meiotic chromatin dynamism in cereals through the visualisation of a homologous barley chromosome arm pair stably transferred into the wheat genetic background. By capturing the dynamics of a single chromosome arm at the same time as detecting the undergoing events of meiotic recombination and synapsis, we showed that subtelomeric chromatin of homologues synchronously transitions to an open chromatin structure during recombination initiation. By contrast, pericentromeric and interstitial regions preserved their closed chromatin organisation and become unpackaged only later, concomitant with initiation of recombinatorial repair and the initial assembly of the synaptonemal complex. Our results raise the possibility that the closed pericentromeric chromatin structure in cereals may influence the fate decision during recombination initiation, as well as the spatial development of synapsis, and may also explain the suppression of crossover events in the proximity of the centromeres.
Arabidopsis non‐host resistance PSS30 gene enhances broad‐spectrum disease resistance in the soybean cultivar Williams 82
Authors: Kambakam Sekhar , Ngaki Micheline N. , Sahu Binod B. , Kandel Devi R. , Singh Prashant , Sumit Rishi , Swaminathan Sivakumar , Muliyar‐Krishna Rajesh , Bhattacharyya Madan K. 
Published: June 2021

SummaryNon‐host resistance (NHR), which protects all members of a plant species from non‐adapted or non‐host plant pathogens, is the most common form of plant immunity. NHR provides the most durable and robust form of broad‐spectrum immunity against non‐adaptive pathogens pathogenic to other crop species. In a mutant screen for loss of Arabidopsis (Arabidopsis thaliana) NHR against the soybean (Glycine max (L.) Merr.) pathogen Phytophthora sojae, the Phytophthora sojae‐susceptible 30 (pss30) mutant was identified. The pss30 mutant is also susceptible to the soybean pathogen Fusarium virguliforme. PSS30 encodes a folate transporter, AtFOLT1, which was previously localized to chloroplasts and implicated in the transport of folate from the cytosol to plastids. We show that two Arabidopsis folate biosynthesis mutants with reduced folate levels exhibit a loss of non‐host immunity against P. sojae. As compared to the wild‐type Col‐0 ecotype, the steady‐state folate levels are reduced in the pss1, atfolt1 and two folate biosynthesis mutants, suggesting that folate is required for non‐host immunity. Overexpression of AtFOLT1 enhances immunity of transgenic soybean lines against two serious soybean pathogens, the fungal pathogen F. virguliforme and the soybean cyst nematode (SCN) Heterodera glycines. Transgenic lines showing enhanced SCN resistance also showed increased levels of folate accumulation. This study thus suggests that folate contributes to non‐host plant immunity and that overexpression of a non‐host resistance gene could be a suitable strategy for generating broad‐spectrum disease resistance in crop plants.
Should I include studies from “predatory” journals in a systematic review? Interim guidance for systematic reviewers
Authors: Munn Zachary , Barker Timothy , Stern Cindy , Pollock Danielle , Ross-White Amanda , Klugar Miloslav , Wiechula Rick , Aromataris Edoardo , Shamseer Larissa 
Published: June 2021

ABSTRACT
A systematic review involves the identification, evaluation, and synthesis of the best-available evidence to provide an answer to a specific question. The “best-available evidence” is, in many cases, a peer-reviewed scientific article published in an academic journal that details the conduct and results of a scientific study. Any potential threat to the validity of these individual studies (and hence the resultant synthesis) must be evaluated and critiqued.
In science, the number of predatory journals continue to rise. Studies published in predatory journals may be of lower quality and more likely to be impacted by fraud and error compared to studies published in traditional journals. This poses a threat to the validity of systematic reviews that include these studies and, therefore, the translation of evidence into guidance for policy and practice. Despite the challenges predatory journals present to systematic reviewers, there is currently little guidance regarding how they should be managed.
In 2020, a subgroup of the JBI Scientific Committee was formed to investigate this issue. In this overview paper, we introduce predatory journals to systematic reviewers, outline the problems they present and their potential impact on systematic reviews, and provide some alternative strategies for consideration of studies from predatory journals in systematic reviews. Options for systematic reviewers could include excluding all studies from suspected predatory journals, applying additional strategies to forensically examine the results of studies published in suspected predatory journals, setting stringent search limits, and applying analytical techniques (such as subgroup or sensitivity analyses) to investigate the impact of suspected predatory journals in a synthesis.
Stress Distribution in a Reentrant Corner
Authors: Brahtz J. H. A. 
Published: June 2021

Abstract
This paper deals with the stress distribution under plain strain in a corner of any angular magnitude, i.e., a plane with an angular incision or notch.
It begins with a brief statement of the method employed by Dr. Th. von Karman in his exact treatment of a beam in bending (Aachen Abhandlungen, Heft 7, 1927).
A generalization of this method is outlined which is applicable to the corner for any force distribution over the straight boundaries, and the solution is found for a concentrated load at any point of the boundary. The stresses are determined and shown to be infinite at the vertex of the corner.
The discussion points out the very interesting paradox that stresses may be finite for certain continuous loadings, but become infinite if a portion of the load is removed.
Estimation of Room-Level Cooling Energy in Hot/Arid Climate by Machine Learning-Based Approaches
Authors: Jia Bingyan , Hou Danlin , Wang Liangzhu (Leon) , Hassan Ibrahim Galal 
Published: June 2021

Abstract
Building energy models (BEM) are developed for understanding a building’s energy performance. A meta-model of the whole building energy analysis is often used for the BEM calibration and energy prediction. The literature review shows that studies with a focus on the development of room-level meta-models are missing. This study aims to address this research gap through a case study of a residential building with 138 apartments in Doha, Qatar. Five parameters, including cooling setpoint, number of occupants, lighting power density, equipment power density, and interior solar reflectance, are selected as input parameters to create ninety-six different scenarios. Three machine-learning models are used as meta-models to generalize the relationship between cooling energy and the model parameters, including Multiple Linear Regression, Support Vector Regression, and Artificial Neural Networks. The three meta-models’ prediction accuracies are evaluated by the Normalized Mean Bias Error (NMBE), Coefficient of Variation of the Root Mean Squared Error CV (RMSE), and R square (R2). The results show that the ANN model performs best. A new generic BEM is then established to validate the meta-model. The results indicate that the proposed meta-model is accurate and efficient in predicting the cooling energy in summer and transitional months for a building with a similar floor configuration.
High School Coaches’ Knowledge and Behaviors for Emergency Preparedness
Authors: Dierickx Erin E. , Scarneo-Miller Samantha E. , Casa Douglas J. 
Published: June 2021

Emergency action plans (EAPs) are considered best practice for the preparation of catastrophic events in sports and, when adequate, can effectively improve patient outcomes. The EAPs should include a collaboration of all personnel involved in the sporting activities, with high school coaches being well-positioned to implement these plans. Therefore, coaches can have a direct influence on the health and safety of their athletes through their involvement in an emergency response. The purpose of this study was to evaluate the understanding and adoption of EAPs as reported by coaches. A web-based questionnaire assessed adoption of an EAP; decisional stages for adoption of components of an EAP; and various characteristics (e.g., access to an athletic trainer facilitators and barriers). A total of 720 responses were included in this study. Nearly 25% of coaches reported being unaware if their school had an EAP, and coaches were more likely to adopt a comprehensive EAP if their school had access to an athletic trainer. A majority of coaches perceived financial assistance as a facilitator and financial limitations as a barrier for EAP implementation. The findings of this investigation illustrate the need for improved coaches’ educational awareness of emergency preparedness strategies within the high school setting.
Is the Polar M430 a Valid Tool for Estimating Maximal Oxygen Consumption in Adult Females?
Authors: Miller Kevin E. , Kempf Timothy R. , Rider Brian C. , Conger Scott A. 
Published: June 2021

Background: Previous research studies have found that heart rate monitors that predict maximal oxygen consumption () are valid for males but overestimate in females. Inaccurate self-reported physical activity (PA) levels may affect the validity of the prediction algorithm used to predict . Purpose: To investigate the validity of the Polar M430 in predicting among females with varying PA levels. Methods: Polar M430 was used to predict () for 43 healthy female study participants (26.9 ± 1.3 years), under three conditions: the participant’s self-selected PA category (sPA), one PA category below the sPA (sPA − 1), and one category above the sPA (sPA + 1). Indirect calorimetry was utilized to measure () via a modified Astrand treadmill protocol. Repeated-measures analyses of covariance using age and percentage of body fat as covariates were used to detect differences between groups. Bland–Altman plots were used to assess the precision of the measurement. Results: was significantly correlated with (r = .695, p < .001). The mean values for and were 44.58 ± 9.29 and 43.98 ± 8.76, respectively. No significant differences were found between , , sPA – 1, and sPA + 1 (p = .492). However, the Bland–Altman plots indicated a low level of precision with the estimate. Conclusions: The Polar M430 was a valid method to predict across different sPA levels in females. Moreover, an under/overestimation in sPA had little effect on the predicted .
Evaluation of two population screening programmes forBRCA1/2founder mutations in the Australian Jewish community: a protocol paper
Authors: Cousens Nicole E , Tiller Jane , Meiser Bettina , Barlow-Stewart Kristine , Rowley Simone , Ko Yi-An , Mahale Sakshi , Campbell Ian G , Kaur Rajneesh , Bankier Agnes , Burnett Leslie , Jacobs Chris , James Paul A , Trainer Alison , Neil Suzanne , Delatycki Martin B , Andrews Lesley 
Published: June 2021

IntroductionPeople of Ashkenazi Jewish (AJ) ancestry are more likely than unselected populations to have aBRCA1/2pathogenic variant, which cause a significantly increased risk of breast, ovarian and prostate cancer. Three specificBRCA1/2pathogenic variants, referred to asBRCA-Jewish founder mutations (B-JFM), account for >90% ofBRCA1/2pathogenic variants in people of AJ ancestry. Current practice of identifying eligible individuals forBRCAtesting based on personal and/or family history has been shown to miss at least 50% of people who have one of these variants. Here we describe the protocol of the JeneScreen study—a study established to develop and evaluate two different population-based B-JFM screening programmes, offered to people of Jewish ancestry in Sydney and Melbourne, Australia.Methods and analysisTo rmeasure the acceptability of population-based B-JFM screening in Australia, two screening programmes using different methodologies have been developed. The Sydney JeneScreen programme provides information and obtains informed consent by way of an online tool. The Melbourne JeneScreen programme does this by way of community sessions attended in person. Participants complete questionnaires to measure clinical and psychosocial outcomes at baseline, and for those who have testing, 2 weeks postresult. Participants who decline testing are sent a questionnaire regarding reasons for declining. Participants with a B-JFM are sent questionnaires 12-month and 24-month post-testing. The questionnaires incorporate validated scales, which measure anxiety, decisional conflict and regret, and test-related distress and positive experiences, and other items specifically developed or adapted for the study. These measures will be assessed for each programme and the two population-based B-JFM screening methods will be compared.Ethics and disseminationInstitutional Human Research Ethics Committee approval was obtained from the South Eastern Area Health Service Human Research Ethics Committee: HREC Ref 16/125.Following the analysis of the study results, the findings will be disseminated widely through conferences and publications, and directly to participants in writing.
Gender-stratified analyses of symptoms associated with acute coronary syndrome in telephone triage: a cross-sectional study
Authors: Wouters Loes T C M , Zwart Dorien L M , Erkelens Daphne C A , De Groot Esther , van Smeden Maarten , Hoes Arno W , Damoiseaux Roger A M J , Rutten Frans H 
Published: June 2021

ObjectivesTo identify clinical variables that are associated with the diagnosis acute coronary syndrome (ACS) in women and men with chest discomfort who contact out-of-hours primary care (OHS-PC) by telephone, and to explore whether there are indications whether these variables differ among women and men.DesignCross-sectional study in which we compared patient and call characteristics of triage call recordings between women with and without ACS, and men with and without ACS.SettingNine OHS-PC in the Netherlands.Participants993 women and 802 men who called OHS-PC for acute chest discomfort (pain, pressure, tightness or discomfort) between 2014 and 2016.Primary outcome measureDiagnosis of ACS retrieved from the patient’s medical record in general practice, including hospital specialists’ discharge letters.ResultsAmong 1795 patients (mean age 58.8 (SD 19.5) years, 55.3% women), 15.0% of men and 8.6% of women had an ACS. In both sexes, retrosternal chest pain was associated with ACS (women with ACS vs without 62.3% vs 40.3%, p=0.002; men with ACS vs without 52.5% vs 39.7%, p=0.032; gender interaction, p=0.323), as was pressing/heavy/tightening pain (women 78.6% vs 61.5%, p=0.011; men 82.1% vs 57.4%, p=<0.001; gender interaction, p=0.368) and radiation to the arm (women 75.6% vs 45.9%, p<0.001; men 56.0% vs 34.8%, p<0.001; gender interaction, p=0.339). Results indicate that only in women were severe pain (65.4% vs 38.1%, p=0.006; gender interaction p=0.007) and radiation to jaw (50.0% vs 22.9%, p=0.007; gender interaction p=0.015) associated with ACS.Ambulances were dispatched equally in women (72.9%) and men with ACS (70.0%).ConclusionOur results indicate there were more similarities than differences in symptoms associated with the diagnosis ACS for women and men. Important exceptions were pain severity and radiation of pain in women. Whether these differences have an impact on predicting ACS needs to be further investigated with multivariable analyses.Trial registration numberNTR7331.
High-intensity strength training in patients with idiopathic inflammatory myopathies: a randomised controlled trial protocol
Authors: Jensen Kasper Yde , Aagaard Per , Schrøder Henrik Daa , Suetta Charlotte , Nielsen Jakob Lindberg , Boyle Eleanor , Diederichsen Louise Pyndt 
Published: June 2021

IntroductionIdiopathic inflammatory myopathies (IIMs) are rare diseases characterised by non-suppurative inflammation of skeletal muscles and muscle weakness. Additionally, IIM is associated with a reduced quality of life. Strength training is known to promote muscle hypertrophy and increase muscle strength and physical performance in healthy young and old adults. In contrast, only a few studies have examined the effects of high intensity strength training in patients with IIM and none using a randomised controlled trial (RCT) set-up. Thus, the purpose of this study is to investigate the effects of high-intensity strength training in patients affected by the IIM subsets polymyositis (PM), dermatomyositis (DM) and immune-mediated necrotising myopathy (IMNM) using an RCT study design.Methods and analysis60 patients with PM, DM or IMNM will be included and randomised into (1) high-intensity strength training or (2) Care-as-Usual. The intervention period is 16 weeks comprising two whole-body strength exercise sessions per week. The primary outcome parameter will be the changes from pre training to post training in the Physical Component Summary measure in the Short Form-36 health questionnaire. Secondary outcome measures will include maximal lower limb muscle strength, skeletal muscle mass, functional capacity, disease status (International Myositis Assessment and Clinical Studies Group core set measures) and questionnaires assessing physical activity levels and cardiovascular comorbidities. Furthermore, blood samples and muscle biopsies will be collected for subsequent analyses.Ethics and disseminationThe study complies with the Helsinki Declaration II and is approved by The Danish Data Protection Agency (P-2020–553). The study is approved by The Danish National Committee on Health Research Ethics (H-20030409). The findings of this trial will be submitted to relevant peer-reviewed journals. Abstracts will be submitted to international conferences.Trial registration numberNCT04486261.
Identifying priority review questions for Cochrane Eyes and Vision: protocol for a priority setting exercise
Authors: Evans Jennifer R , Gordon Iris , Lawrenson John G , Parker Roses , Rowe Fiona J , Virgili Gianni , Li Tianjing , Ramke Jacqueline 
Published: June 2021

IntroductionCochrane Eyes and Vision (CEV) is an international network of individuals working to prepare, maintain and promote access to systematic reviews of interventions to treat, prevent or diagnose eye diseases or vision impairment. CEV plans to undertake a priority setting exercise to identify systematically research questions relevant to our scope, and to formally incorporate input from a wide range of stakeholders to set priorities for new and updated reviews.Methods and analysisThe scope of CEV is broad and our reviews include conditions that are common and have a high global disease burden, for example, cataract and dry eye disease, and conditions that are rare but have a high impact on quality of life and high individual cost such as eye cancer. We plan to focus on conditions prioritised by WHO during the development of the Package of Eye Care Interventions. These conditions were selected based on a combination of data on disease magnitude, healthcare use and expert opinion. We will identify priority review questions systematically by summarising relevant data on research in Eyes and Vision from a range of sources, and compiling a list of 10–15 potential review questions (new and/or updates) for each condition group. We will seek the views of external and internal stakeholders on this list by conducting an online survey. Equity will be a specific consideration.Ethics and disseminationThe study has been approved by the ethics committee of the London School of Hygiene & Tropical Medicine. We will disseminate the findings through Cochrane channels and prepare a summary of the work for publication in a peer-reviewed journal.