bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stress reactivity as a putative mechanism linking childhood trauma with clinical outcomes in individuals at ultra-high-risk for psychosis: Findings from the EU-GEI High Risk Study
Authors: Paetzold I. , Myin-Germeys I. , Schick A. , Nelson B. , Velthorst E. , Schirmbeck F. ,   EU-GEI High Risk Study, van Os J. , Morgan C. , Hartmann J. , van der Gaag M. , de Haan L. , Valmaggia L. , McGuire P. , Kempton M. , Reininghaus U. 
Published: May 2021

Abstract

Aims
Childhood trauma is associated with an elevated risk for psychosis, but the psychological mechanisms involved remain largely unclear. This study aimed to investigate emotional and psychotic stress reactivity in daily life as a putative mechanism linking childhood trauma and clinical outcomes in individuals at ultra-high-risk (UHR) for psychosis.

Methods
Experience sampling methodology was used to measure momentary stress, affect and psychotic experiences in the daily life of N = 79 UHR individuals in the EU-GEI High Risk Study. The Childhood Trauma Questionnaire was used to assess self-reported childhood trauma. Clinical outcomes were assessed at baseline, 1- and 2-year follow-up.

Results
The association of stress with positive (β = −0.14, p = 0.010) and negative affect (β = 0.11, p = 0.020) was modified by transition status such that stress reactivity was greater in individuals who transitioned to psychosis. Moreover, the association of stress with negative affect (β = 0.06, p = 0.019) and psychotic experiences (β = 0.05, p = 0.037) was greater in individuals exposed to high v. low levels of childhood trauma. We also found evidence that decreased positive affect in response to stress was associated with reduced functioning at 1-year follow-up (B = 6.29, p = 0.034). In addition, there was evidence that the association of childhood trauma with poor functional outcomes was mediated by stress reactivity (e.g. indirect effect: B = −2.13, p = 0.026), but no evidence that stress reactivity mediated the association between childhood trauma and transition (e.g. indirect effect: B = 0.14, p = 0.506).

Conclusions
Emotional and psychotic stress reactivity may be potential mechanisms linking childhood trauma with clinical outcomes in UHR individuals.
Labor Market Volatility, Gender, and Trade Preferences
Authors: Brutger Ryan , Guisinger Alexandra 
Published: May 2021

AbstractWhat explains divides in the public’s support for trade protection? Traditional economic arguments primarily focus on individuals’ expectations for increased or decreased wages in the face of greater economic openness, yet studies testing such wage-based concerns identify a different divide as well: even after accounting for wage effects, women are typically more supportive of trade protection. We argue that trade-induced employment volatility and the resulting concerns for employment stability are overlooked factors that help explain the gender divide in attitudes. Due to both structural discrimination and societal norms, we theorize that working women are more responsive to the threat of trade-related employment instability than male counterparts. Using an experiment fielded on national samples in the USA and Canada, we find that most respondents have weak reactions to volatility, but volatility has a significant effect on women who are the most vulnerable to trade’s disruptive effects – those working in import-competing industries and those with limited education.
Hate Speech as an Indicator for the State of the	Society
Authors: Schäfer Svenja , Sülflow Michael , Reiners Liane 
Published: May 2021

Abstract. Previous research indicates that user comments serve as exemplars and thus have an effect on perceived public opinion. Moreover, they also shape the attitudes of their readers. However, studies almost exclusively focus on controversial issues if they explore the consequences of user comments for attitudes and perceived public opinion. The current study wants to find out if hate speech attacking social groups due to characteristics such as religion or sexual orientation also has an effect on the way people think about these groups and how they think society perceives them. Moreover, we also investigated the effects of hate speech on prejudiced attitudes. To explore the hypotheses and research questions, we preregistered and conducted a 3 × 2 experimental study varying the amount of hate speech (none/few/many hateful comments) and the group that was attacked (Muslims/homosexuals). Results show no effects of the amount of hate speech on perceived public opinion for both groups. However, if homosexuals are attacked, hate speech negatively affects perceived social cohesion. Moreover, for both groups, we find interaction effects between preexisting attitudes and hate speech for discriminating demands. This indicates that hate speech can increase polarization in society.
Subgrain Boundaries as Sites of Melt Generation and Pathways for Melt Flow: Implications for Rheology From Combined Chemical and Quantitative Orientation Analysis
Authors: Levine J. S. F. , Rahl J. M. 
Published: May 2021

AbstractWe report a detailed petrographic, compositional, structural, and crystallographic investigation into the partial melting process. Migmatitic samples that record biotite‐dehydration melting in the Wet Mountains of central Colorado preserve textural evidence for partial melting locations and an incipient former melt network. Detailed analysis of partial melting locations was conducted using a combined energy dispersive X‐ray spectroscopy (EDS) and electron backscatter diffraction (EBSD) approach. Cuspate‐shaped films of former melt, now plagioclase, with low apparent dihedral angles and adjacent to rounded and corroded reactant minerals are interpreted as former locations of partial melting. We identified former melt films infiltrating and melting along quartz grains using optical microscopy and EDS and determined crystallographic misorientation across these transects using EBSD. Forty‐two percent of these transects are associated with subgrain boundaries, and the remaining transects are between adjacent quartz grains with various misorientations. Although previous work has emphasized grain boundaries and triple junctions as sites for melting, the presence of over 40% of the former melt films developed along subgrain boundaries suggests that these are favorable and potentially preferred locations for partial melting. Consequently, melting along subgrain boundaries must be integral to the evolution of partial melt networks and will increase melt network interconnectedness and generate a finer grain size, enhancing permeability and leading to focused melt flow. Given that these factors exert a fundamental control on crustal rheology, the role of melting and migration along subgrain boundaries should be investigated within diverse crustal and mantle lithologies and incorporated into models of partial melting.
Convergent Hydraulic Redistribution and Groundwater Access Supported Facilitative Dependency Between Trees and Grasses in a Semi‐Arid Environment
Authors: Lee E. , Kumar P. , Knowles J. F. , Minor R. L. , Tran N. , Barron‐Gafford G. A. , Scott R. L. 
Published: May 2021

AbstractHydraulic redistribution is the transport of water from wet to dry soil layers, upward or downward, through plant roots. Often in savanna and woodland ecosystems, deep‐rooted trees, and shallow‐rooted grasses coexist. The degree to which these different species compete for or share soil‐water derived from precipitation or groundwater, as well as how these interactions are altered by hydraulic redistribution, is unknown. We use a multilayer canopy model and field observations to examine how the presence of deep, but tree‐root accessible, groundwater impacts seasonal patterns of hydraulic redistribution, and interaction between coexisting vegetation species in a semiarid riparian woodland (US‐CMW). Based on the simulation, trees absorb moisture at the water table (∼10 m depth) and release it in the shallow soil depth (0–3 m) during the dry pre‐monsoon season. We observed the occurrence of a new convergent hydraulic redistribution pattern during the monsoon season, where moisture is transported from both the near‐surface (0–0.5 m) and the water table to intermediate soil layers (1–5 m) through tree roots. We found that hydraulic redistribution demonstrates a growth facilitation effect at this site, supporting 49% of growing season tree transpiration and 14% of the grass transpiration. Compared to a similarly structured upland savanna without accessible groundwater, the riparian site shows an increased amount of hydraulically redistributed water and more facilitative water use between coexisting grasses and trees. These results shed light on the linkage between accessible groundwater and the role of hydraulic redistribution on the interaction between deep‐rooted and shallow‐rooted vegetation.
Low‐Temperature Hydrogen Formation During Aqueous Alteration of Serpentinized Peridotite in the Samail Ophiolite
Authors: Ellison Eric T. , Templeton Alexis S. , Zeigler Spencer D. , Mayhew Lisa E. , Kelemen Peter B. , Matter Juerg M. ,   The Oman Drilling Project Science Party
Published: May 2021

AbstractSerpentinized peridotite is reacting with groundwater in the subsurface of the Samail ophiolite in Oman. Although these rocks are partially to completely serpentinized, they interact with a groundwater aquifer containing hyperalkaline fluids rich in H2 and CH4. Since the mechanisms by which H2 production may continue at low temperatures (<50°C) are not fully understood, core recovered during the Oman Drilling Project provides an excellent opportunity to study the mineralogy and Fe speciation in highly serpentinized harzburgite recording multiple stages of water/rock interaction. In Hole BA3A, early hydration of olivine and pyroxene, which likely occurred at temperatures of ∼100°C–200°C, produced serpentine, Fe‐rich brucite, awaruite, and little magnetite. Notably, Fe‐rich brucite is only preserved at >∼100 m depth in the core. Fe‐rich brucite is nearly absent within two near‐surface reaction zones where later stages of reaction are recorded, which include replacement of Fe‐rich brucite by Fe(III)‐bearing serpentine, and increases in the proportion of other Fe(III)‐bearing phases such as magnetite and hydroandradite. Thus, Fe‐rich brucite at depth represents substantial stored capacity for H2 production that can continue at low temperature, even after primary olivine and pyroxene are exhausted, thereby sustaining habitable conditions for microbial life.
Evaluation of CMIP6 Global Climate Models for Simulating Land Surface Energy and Water Fluxes During 1979–2014
Authors: Li Jianduo , Miao Chiyuan , Wei Wei , Zhang Guo , Hua Lijuan , Chen Yueli , Wang Xiaoxiao 
Published: May 2021

AbstractThis study examined the overall performance of the climate models in Phase 6 of the Coupled Model Intercomparison Project (CMIP6) in simulating the key energy and water fluxes over land. For this purpose, this study selected multiple land flux products as reference data sets and assessed the global spatial means, patterns, trends, seasonal cycles, and regional mean estimates of the sensible heat (SH), latent heat (LH), net radiation (RN), runoff (RF), and precipitation (PR) simulated by 32 CMIP6 models in recent decades. The global (Antarctica, Greenland, and hot deserts are not included) mean SH, LH, RN, RF, and PR simulated by the CMIP6 models are 37.55 ± 4.81 W m−2, 49.88 ± 5.31 W m−2, 89.10 ± 4.45 W m−2, 351.31 ± 95.28 mm yr−1, and 948.35 ± 88.77 mm yr−1, respectively. The ensemble median of CMIP6 simulations (CMIP6‐MED) can provide robust estimates of global and regional land fluxes, which are within the ranges given by the reference data sets, and highly consistent spatiotemporal patterns of these fluxes. The comparison of CMIP6‐MED with the first preferred reference data sets shows that CMIP6‐MED generally overestimates the water and energy fluxes over land, except for the simulated RF and PR in the Amazon region. The most disagreements between CMIP6‐MED and the reference data sets occur in South America (particularly the Amazon region) and the Tibetan Plateau. Finally, the sources of model biases are discussed. It is suggested that current land flux products should be widely used to optimize the structures and parameters of climate models in future work.
Cyst(e)ine in nutrition formulation promotes colon cancer growth and chemoresistance by activating mTORC1 and scavenging ROS
Authors: Wu Jiao , Yeung Sai-Ching Jim , Liu Sicheng , Qdaisat Aiham , Jiang Dewei , Liu Wenli , Cheng Zhuo , Liu Wenjing , Wang Haixia , Li Lu , Zhou Zhongmei , Liu Rong , Yang Chuanyu , Chen Ceshi , Yang Runxiang 
Published: May 2021

AbstractWeight loss and cachexia are common problems in colorectal cancer patients; thus, parenteral and enteral nutrition support play important roles in cancer care. However, the impact of nonessential amino acid components of nutritional intake on cancer progression has not been fully studied. In this study, we discovered that gastrointestinal cancer patients who received cysteine as part of the parenteral nutrition had shorter overall survival (P < 0.001) than those who did not. Cystine indeed robustly promotes colon cancer cell growth in vitro and in immunodeficient mice, predominately by inhibiting SESN2 transcription via the GCN2-ATF4 axis, resulting in mTORC1 activation. mTORC1 inhibitors Rapamycin and Everolimus block cystine-induced cancer cell proliferation. In addition, cystine confers resistance to oxaliplatin and irinotecan chemotherapy by quenching chemotherapy-induced reactive oxygen species via synthesizing glutathione. We demonstrated that dietary deprivation of cystine suppressed colon cancer xenograft growth without weight loss in mice and boosted the antitumor effect of oxaliplatin. These findings indicate that cyst(e)ine, as part of supplemental nutrition, plays an important role in colorectal cancer and manipulation of cyst(e)ine content in nutritional formulations may optimize colorectal cancer patient survival.
Enzyme promiscuity in natural environments: alkaline phosphatase in the ocean
Authors: Srivastava Abhishek , Saavedra Daniel E M , Thomson Blair , García Juan A L , Zhao Zihao , Patrick Wayne M , Herndl Gerhard J , Baltar Federico 
Published: May 2021

Abstract
Alkaline phosphatase (APase) is one of the marine enzymes used by oceanic microbes to obtain inorganic phosphorus (Pi) from dissolved organic phosphorus to overcome P-limitation. Marine APase is generally recognized to perform P-monoesterase activity. Here we integrated a biochemical characterization of a specific APase enzyme, examination of global ocean databases, and field measurements, to study the type and relevance of marine APase promiscuity. We performed an in silico mining of phoA homologs, followed by de novo synthesis and heterologous expression in E. coli of the full-length gene from Alteromonas mediterranea, resulting in a recombinant PhoA. A global analysis using the TARA Oceans, Malaspina and other metagenomic databases confirmed the predicted widespread distribution of the gene encoding the targeted PhoA in all oceanic basins throughout the water column. Kinetic assays with the purified PhoA enzyme revealed that this enzyme exhibits not only the predicted P-monoester activity, but also P-diesterase, P-triesterase and sulfatase activity as a result of a promiscuous behavior. Among all activities, P-monoester bond hydrolysis exhibited the highest catalytic activity of APase despite its lower affinity for phosphate monoesters. APase is highly efficient as a P-monoesterase at high substrate concentrations, whereas promiscuous activities of APase, like diesterase, triesterase, and sulfatase activities are more efficient at low substrate concentrations. Strong similarities were observed between the monoesterase:diesterase ratio of the purified PhoA protein in the laboratory and in natural seawater. Thus, our results reveal enzyme promiscuity of APase playing potentially an important role in the marine phosphorus cycle.
Theta burst stimulation for the acute treatment of major depressive disorder: A systematic review and meta-analysis
Authors: Voigt Jeffrey D. , Leuchter Andrew F. , Carpenter Linda L. 
Published: May 2021

AbstractPatients with major depressive disorder (MDD) may be refractory to or have contraindications that preclude treatment with antidepressant pharmacotherapies. Alternative therapies such as repetitive transcranial magnetic stimulation (rTMS) continue to evolve, and include theta burst stimulation (TBS), which has advantages over conventional rTMS. The aim of this study was to identify and meta-analyze efficacy data from all randomized controlled trials (RCTs) investigating TBS as a treatment for MDD. Published reports of RCTs (January 1, 2010 to October 23, 2020) were identified via systematic searches in computerized databases, followed by review of individual reports for inclusion. Inclusion criteria included primary diagnosis of MDD ≥ 1 week duration of therapy with ≥10 sessions, and treatment with any form of TBS. The Cochrane GRADE methodology and PRISMA criteria were used for evaluation of individual trials. Data from ten RCTs were included, representing 667 patients. Of these, 8 RCTs compared TBS to sham treatment and one compared TBS to standard rTMS (i.e., high frequency stimulation over left dorsolateral prefrontal cortex [HFL]). Quality of evidence assessment yielded high confidence in the finding of TBS being superior to sham on response measured by the Hamilton Depression Rating Scale (HRSD) (RR = 2.4; 95% CI: 1.27 to 4.55; P = 0.007; I2 = 40%). Comparison of HRSD response rates for TBS versus rTMS produced no statistically significant difference (RR = 1.02; 95% CI: 0.85 to 1.23; P = 0.80; I2 = 0%). The incidence of adverse events between TBS and rTMS was not statistically different. The findings of a positive effect of TBS vs. sham, and noninferiority of TBS vs. standard HFL rTMS support the continued development of TBS to treat depression.
A single serving of mixed spices alters gut microflora composition: a dose–response randomised trial
Authors: Khine Wei Wei Thwe , Haldar Sumanto , De Loi Shou , Lee Yuan-Kun 
Published: May 2021

AbstractShort-term changes in dietary intake can induce changes in gut microbiome. While various dietary polyphenols have been shown to modulate gut microflora, the acute influence of polyphenol-rich mixed spices has not been explored in a controlled setting. We investigated the effects of a single serving of mixed spices Indian curry consumption, in two separate doses, on the gut microbiome in 15 healthy, Singaporean Chinese males, with age and BMI of 23.5 ± 2.4 years and 22.9 ± 2.2 kg/m2 respectively. We found that a low-polyphenol, no spices Dose 0 Control (D0C) meal led to an increase in Bacteroides and a decrease in Bifidobacterium. In comparison to D0C, there was significant suppression of Bacteroides (p < 0.05) and an increase in Bifidobacterium (p < 0.05) with increasing doses of curry meal Dose 1 Curry (D1C) and Dose 2 Curry (D2C) containing 6 g and 12 g mixed spices respectively. Significant correlations were also found between bacterial changes and plasma phenolic acids. No differences between treatments were observed in the alpha-diversity of the gut microflora. This study has shown that a single serving of mixed spices can significantly modify/restore certain commensal microbes, particularly in people who do not regularly consume these spices.
Abnormal﻿ composition of microbiota in the gut and skin of imiquimod-treated mice
Authors: Shinno-Hashimoto Hiroyo , Hashimoto Yaeko , Wei Yan , Chang Lijia , Fujita Yuko , Ishima Tamaki , Matsue Hiroyuki , Hashimoto Kenji 
Published: May 2021

AbstractPsoriasis is a chronic, inflammatory skin disease. Although the precise etiology of psoriasis remains unclear, gut–microbiota axis might play a role in the pathogenesis of the disease. Here we investigated whether the composition of microbiota in the intestine and skin is altered in the imiquimod (IMQ)-treated mouse model of psoriasis. Topical application of IMQ to back skin caused significant changes in the composition of microbiota in the intestine and skin of IMQ-treated mice compared to control mice. The LEfSe algorithm identified the species Staphylococcus lentus as potential skin microbial marker for IMQ group. Furthermore, there were correlations for several microbes between the intestine and skin, suggesting a role of skin–gut–microbiota in IMQ-treated mice. Levels of succinic acid and lactic acid in feces from IMQ-treated mice were significantly higher than control mice. Moreover, the predictive functional analysis of the microbiota in the intestine and skin showed that IMQ caused alterations in several KEGG pathways. In conclusion, the current data indicated that topical application with IMQ to skin alters the composition of the microbiota in the gut and skin of host. It is likely that skin–gut microbiota axis plays a role in pathogenesis of psoriasis.
Genetic and antigenic evolution of H1 swine influenza A viruses isolated in Belgium and the Netherlands from 2014 through 2019
Authors: Chepkwony Sharon , Parys Anna , Vandoorn Elien , Stadejek Wojciech , Xie Jiexiong , King Jacqueline , Graaf Annika , Pohlmann Anne , Beer Martin , Harder Timm , Van Reeth Kristien 
Published: May 2021

AbstractSurveillance of swine influenza A viruses (swIAV) allows timely detection and identification of new variants with potential zoonotic risks. In this study, we aimed to identify swIAV subtypes that circulated in pigs in Belgium and the Netherlands between 2014 and 2019, and characterize their genetic and antigenic evolution. We subtyped all isolates and analyzed hemagglutinin sequences and hemagglutination inhibition assay data for H1 swIAV, which were the dominant HA subtype. We also analyzed whole genome sequences (WGS) of selected isolates. Out of 200 samples, 89 tested positive for swIAV. swIAV of H1N1, H1N2 and H3N2 subtypes were detected. Analysis of WGS of 18 H1 swIAV isolates revealed three newly emerged genotypes. The European avian-like H1 swIAV (lineage 1C) were predominant and accounted for 47.2% of the total isolates. They were shown to evolve faster than the European human-like H1 (1B lineage) swIAV, which represented 27% of the isolates. The 2009 pandemic H1 swIAV (lineage 1A) accounted for only 5.6% of the isolates and showed divergence from their precursor virus. These results point to the increasing divergence of swIAV and stress the need for continuous surveillance of swIAV.