bluuebunny/crossref_metadata_2025_split huggingface dataset:
Roles of HDAC3-orchestrated circadian clock gene oscillations in diabetic rats following myocardial ischaemia/reperfusion injury
Authors: Qiu Zhen , Ming Hao , Lei Shaoqing , Zhou Bin , Zhao Bo , Yu Yanli , Xue Rui , Xia Zhongyuan 
Published: January 2021

AbstractThe circadian clock is closely related to the development of diabetes mellitus and cardiovascular disease, and disruption of the circadian clock exacerbates myocardial ischaemia/reperfusion injury (MI/RI). HDAC3 is a key component of the circadian negative feedback loop that controls the expression pattern of the circadian nuclear receptor Rev-erbα to maintain the stability of circadian genes such as BMAL1. However, the mechanism by which the HDAC3-orchestrated Rev-erbα/BMAL1 pathway increases MI/RI in diabetes and its relationship with mitophagy have yet to be elucidated. Here, we observed that the clock genes Rev-erbα, BMAL1, and C/EBPβ oscillations were altered in the hearts of rats with streptozotocin (STZ)-induced diabetes, with upregulated HDAC3 expression. Oscillations of Rev-erbα and BMAL1 were rapidly attenuated in diabetic MI/R hearts versus non-diabetic I/RI hearts, in accordance with impaired and rhythm-disordered circadian-dependent mitophagy that increased injury. Genetic knockdown of HDAC3 significantly attenuated diabetic MI/RI by mediating the Rev-erbα/BMAL1 circadian pathway to recover mitophagy. Primary cardiomyocytes with or without HDAC3 siRNA and Rev-erbα siRNA were exposed to hypoxia/reoxygenation (H/R) in vitro. The expression of HDAC3 and Rev-erbα in cardiomyocytes was increased under high-glucose conditions compared with low-glucose conditions, with decreased BMAL1 expression and mitophagy levels. After H/R stimulation, high glucose aggravated H/R injury, with upregulated HDAC3 and Rev-erbα expression and decreased BMAL1 and mitophagy levels. HDAC3 and Rev-erbα siRNA can alleviate high glucose-induced and H/R-induced injury by upregulating BMAL1 to increase mitophagy. Collectively, these findings suggest that disruption of HDAC3-mediated circadian gene expression oscillations induces mitophagy dysfunction, aggravating diabetic MI/RI. Cardiac-specific HDAC3 knockdown could alleviate diabetic MI/RI by regulating the Rev-erbα/BMAL1 pathway to restore the activation of mitophagy.
ATF4-mediated histone deacetylase HDAC1 promotes the progression of acute pancreatitis
Authors: Deng Xiaofeng , He Yu , Miao Xiongying , Yu Bo 
Published: January 2021

AbstractAcute pancreatitis (AP), an acute inflammatory process, can be difficult to diagnose. Activating transcription factor 4 (ATF4) has been reported to participate in the pathogenesis of AP. Additionally, histone deacetylases (HDACs) are shown to be closely related to the development of a variety of diseases, including inflammation disease. In our study, we tried to highlight the role of ATF4 in AP through regulation of HDAC1. Firstly, we validated the effect of ATF4 on pancreatic acinar cell proliferation, apoptosis, and inflammation through in vitro experiments on cellular models of caerulein-induced AP. Next, we examined the correlation between ATF4 and HDAC1, and between HDAC1 with neutral endopeptidase (NEP) and kruppel-like factor 4 (KLF4). Finally, the regulatory role of ATF4 in AP was further assessed by determination of pathological conditions, biochemical indicators and inflammation through in vivo experiments on caerulein-induced AP mouse models. After AP induction, highly expressed ATF4 was observed, and silencing ATF4 could promote pancreatic acinar cell proliferation and inhibit apoptosis. ATF4 could bind to the HDAC1 promoter and upregulate its expression in AP. Moreover, HDAC1 could increase KLF4 expression by inhibiting NEP expression. Functionally, silencing ATF4 could suppress AP through regulation of NEP-mediated KLF4 via downregulation of HDAC1. Above all, our study uncovered the promotive role of ATF4 in AP through upregulation of HDAC1.
Long noncoding RNA LINC00941 promotes pancreatic cancer progression by competitively binding miR-335-5p to regulate ROCK1-mediated LIMK1/Cofilin-1 signaling
Authors: Wang Jie , He Zhiwei , Xu Jian , Chen Peng , Jiang Jianxin 
Published: January 2021

AbstractAn accumulation of evidence indicates that long noncoding RNAs are involved in the tumorigenesis and progression of pancreatic cancer (PC). In this study, we investigated the functions and molecular mechanism of action of LINC00941 in PC. Quantitative PCR was used to examine the expression of LINC00941 and miR-335-5p in PC tissues and cell lines, and to investigate the correlation between LINC00941 expression and clinicopathological features. Plasmid vectors or lentiviruses were used to manipulate the expression of LINC00941, miR-335-5p, and ROCK1 in PC cell lines. Gain or loss-of-function assays and mechanistic assays were employed to verify the roles of LINC00941, miR-335-5p, and ROCK1 in PC cell growth and metastasis, both in vivo and in vitro. LINC00941 and ROCK1 were found to be highly expressed in PC, while miR-335-5p exhibited low expression. High LINC00941 expression was strongly associated with larger tumor size, lymph node metastasis, and poor prognosis. Functional experiments revealed that LINC00941 silencing significantly suppressed PC cell growth, metastasis and epithelial–mesenchymal transition. LINC00941 functioned as a molecular sponge for miR-335-5p, and a competitive endogenous RNA (ceRNA) for ROCK1, promoting ROCK1 upregulation, and LIMK1/Cofilin-1 pathway activation. Our observations lead us to conclude that LINC00941 functions as an oncogene in PC progression, behaving as a ceRNA for miR-335-5p binding. LINC00941 may therefore have potential utility as a diagnostic and treatment target in this disease.
Snai1-induced partial epithelial–mesenchymal transition orchestrates p53–p21-mediated G2/M arrest in the progression of renal fibrosis via NF-κB-mediated inflammation
Authors: Qi Ruochen , Wang Jiyan , Jiang Yamei , Qiu Yue , Xu Ming , Rong Ruiming , Zhu Tongyu 
Published: January 2021

AbstractRenal fibrosis is the common feature of all progressive kidney diseases and exerts great burden on public health worldwide. The maladaptive repair mechanism of tubular epithelial cells, an important mediator of renal fibrogenesis, manifests with partial epithelial–mesenchymal transition (EMT) and cell cycle arrest. The aim of this study is to investigate the possible correlation between partial EMT and cell cycle arrest, and elucidate the underlying mechanism. We examined human kidney allograft samples with interstitial fibrosis and three mice renal fibrosis models, unilateral ureter obstruction (UUO), ischemia–reperfusion injury, and Adriamycin nephropathy. The partial EMT process and p53–p21 axis were elevated in both human allograft with interstitial fibrosis, as well as three mice renal fibrosis models, and showed a time-dependent increase as fibrosis progressed in the UUO model. Snai1 controlled the partial EMT process, and led to parallel changes in renal fibrosis, G2/M arrest, and inflammation. p53–p21 axis arrested cell cycle at G2/M, and prompted partial EMT and fibrosis together with inflammation. NF-κB inhibitor Bay11-7082 disrupted the reciprocal loop between Snai1-induced partial EMT and p53–p21-mediated G2/M arrest. We demonstrated the reciprocal loop between partial EMT and G2/M arrest of TECs during renal fibrogenesis and revealed NF-κB-mediated inflammatory response as the underlying mechanism. This study suggests that targeting NF-κB might be a plausible therapeutic strategy to disrupt the reciprocal loop between partial EMT and G2/M arrest, therefore alleviating renal fibrosis.
Gridded fossil CO2 emissions and related O2 combustion consistent with national inventories 1959–2018
Authors: Jones Matthew W. , Andrew Robbie M. , Peters Glen P. , Janssens-Maenhout Greet , De-Gol Anthony J. , Ciais Philippe , Patra Prabir K. , Chevallier Frederic , Le Quéré Corinne 
Published: January 2021

AbstractQuantification of CO2 fluxes at the Earth’s surface is required to evaluate the causes and drivers of observed increases in atmospheric CO2 concentrations. Atmospheric inversion models disaggregate observed variations in atmospheric CO2 concentration to variability in CO2 emissions and sinks. They require prior constraints fossil CO2 emissions. Here we describe GCP-GridFED (version 2019.1), a gridded fossil emissions dataset that is consistent with the national CO2 emissions reported by the Global Carbon Project (GCP). GCP-GridFEDv2019.1 provides monthly fossil CO2 emissions estimates for the period 1959–2018 at a spatial resolution of 0.1°. Estimates are provided separately for oil, coal and natural gas, for mixed international bunker fuels, and for the calcination of limestone during cement production. GCP-GridFED also includes gridded estimates of O2 uptake based on oxidative ratios for oil, coal and natural gas. It will be updated annually and made available for atmospheric inversions contributing to GCP global carbon budget assessments, thus aligning the prior constraints on top-down fossil CO2 emissions with the bottom-up estimates compiled by the GCP.
Galaxy and Mass Assembly: Group and field galaxy morphologies in the star-formation rate – stellar mass plane
Authors: Pearson W. J. , Wang L. , Brough S. , Holwerda B. W. , Hopkins A. M. , Loveday J. 
Published: January 2021

Aims. We study the environment in which a galaxy lies (i.e. field or group) and its connection with the morphology of the galaxy. This is done by examining the distribution of parametric and non-parametric statistics across the star-formation rate (SFR) – stellar mass (M⋆) plane and studying how these distributions change with the environment in the local universe (z < 0.15).
Methods. We determine the concentration (C), Gini, M20, asymmetry, Gini-M20 bulge statistic (GMB), 50% light radius (r50), total Sérsic index, and bulge Sérsic index (nBulge) for galaxies from the Galaxy and Mass Assembly (GAMA) survey using optical images from the Kilo Degree Survey. We determine the galaxy environment using the GAMA group catalogue and split the galaxies into field or group galaxies. The group galaxies are further divided by the group halo mass (Mh)−11 ≤ log(Mh/M⊙) < 12, 12 ≤ log(Mh/M⊙) < 13, and 13 ≤ log(Mh/M⊙) < 14 – and into central and satellite galaxies. The galaxies in each of these samples are then placed onto the SFR-M⋆ plane, and each parameter is used as a third dimension. We fit the resulting distributions for each parameter in each sample using two two-dimensional Gaussian distributions: one for star-forming galaxies and one for quiescent galaxies. The coefficients of these Gaussian fits are then compared between environments.
Results. Using C and r50, we find that galaxies typically become larger as the group mass increases. This change is greater for larger galaxies. There is no indication that galaxies are typically more or less clumpy as the environment changes. Using GMB and nBulge, we see that the star-forming galaxies do not become more bulge or disk dominated as the group mass changes. Asymmetry does not appear to be greatly influenced by environment.
Comparison of Value Added within EU in Terms of Corporate Investment in Research and Development
Authors: Kostiuk Yaroslava , Korená Kristína 
Published: January 2021

Globalization has accelerated the growth of innovation in many countries outside the European Union. In order to remain competitive, companies and individual EU economies therefore support the development of innovations through investments in research and development.
This contribution is focused on the specification of value added in terms of investments in research and development in the construction and manufacturing sectors. Using statistics of the European Commission for the period 2013 – 2017, a dataset of 269,892 companies in the EU28 countries was generated.
The purpose of this contribution is to determine to what extent investment in research and development participates in the creation of value added. A research question was formulated to determine the ideal ratio between value added and investment.
Methods: To achieve the objectives set, regression analysis of neural networks was applied using Statistica software.
The results of the research indicate that the optimal values in the construction sector were achieved by Great Britain (~EUR 11.3 billion.) and France (~EUR 16.1 billion), and in the manufacturing sector by France (~EUR 11.42 billion), Italy (~EUR 11.41 billion) and Great Britain (~EUR 10.6 billion).
The authors consider the method of regression analysis and neural networks to be appropriate for examining the optimal ratio between investment in R&D and value added.
The NF-kB and Collagen Type 1 Expression in Dental Pulp after Treated Calcium Hydroxide Combined with Propolis
Authors: Pribadi Nirawati , Budiarti Dwita , Kurniawan Hendy Jaya , Widjiastuti Ira 
Published: January 2021

AbstractObjective To analyze the expression of nuclear factor kappa B (NF-kB) and collagen type 1 on dental pulp after a treated combination of calcium hydroxide and propolis.Materials and Methods The first maxillary molars of 30 rats were mechanically perforated. Teeth were divided into three groups of 10 for two separate extraction time frames, giving a total of 60 rats. The control groups were treated with Cention, the second treatment groups were treated with calcium hydroxide, and the third treatment groups were treated with a combination of calcium hydroxide and propolis. Final restoration was done with Cention. The teeth were extracted on days 7 and 14, and the expression of NF-kB and collagen type I was analyzed using immunohistochemistry.Results There is lowest NF-kB expression and highest collagen type 1 expression on dental pulp after treated with a combination of calcium hydroxide and propolis on days 7 and 14 (p < 0.05).Conclusion The combination of calcium hydroxide and propolis inhibits pulp inflammation and stimulates regeneration through decreasing the NF-kB expression and increasing collagen type 1.
Inhibitor Index in the Clot Waveform Analysis-Based Mixing Test Differentiates among Hemophilia A without and with Inhibitors, and Lupus Anticoagulant
Authors: Shimonishi Naruto , Ogiwara Kenichi , Oda Yukio , Kawabe Toshiki , Emmi Mari , Shima Midori , Nogami Keiji 
Published: January 2021

Abstract
Background The mixing test is used to identify the pathway to follow-up testing and is also useful for the investigation of lupus anticoagulant (LA) positivity. “To completely correct” indicates clotting factor deficiency, while “to not correct” indicates the presence of a clotting factor inhibitor including LA. “Index of circulation anticoagulant” and/or “percent correction” is used to interpret the results of mixing studies, but it does not accurately differentiate factor inhibitors from LA.
Aim To precisely differentiate hemophilia A (HA), HA with inhibitor (HA-inh), and LA using the clot waveform analysis (CWA)-based mixing test.
Methods Plasma samples from HA, LA, and HA-inh including acquired HA were incubated with normal plasma in 9:1, 1:1, and 1:9 mix ratios. From activated partial thromboplastin time CWA at 0-minute (immediately) and 12-minute incubation, the ratios of CWA parameters at 12 minutes/0 minute (inhibitor index) were assessed.
Results The inhibitor index values of CWA parameters obtained using the mixing test in a 1:1 ratio demonstrated a significant difference between HA-inh and LA but could not differentiate LA from HA-inh completely. Plasmas used for the mixing tests in 9:1 and 1:9 ratios were able to fully distinguish between HA-inh (>0.5 BU/mL) and LA. These indices significantly correlated with inhibitor titer below 40 BU/mL (r > 0.90), possibly estimating FVIII inhibitor titer from the inhibitor index. Plasmas in HA and LA could be distinguished by mixing in a 1:1 ratio at 0 minute (immediately).
Conclusion The inhibitor index from CWA-based mixing tests with a 12-minute incubation could differentiate among HA, HA-inh, and LA quickly.
A Case of Sudden Diffuse Thyroid Swelling after Fine Needle Aspiration Cytology where Changes over Time Were Confirmed by Ultrasonography
Authors: Takemoto Naoki , Ozawa Taijiro , Tamai Hitomi , Asaoka Tatsuhiro , Iwasaki Shinichi 
Published: January 2021

AbstractWe encountered a case of diffuse thyroid swelling after fine needle aspiration (dTSaFNA) cytology, and monitored the swelling by thyroid ultrasonography. The scan findings are reported. A 57-year-old female visited our hospital complaining of swelling in her anterior neck. She had a history of metal allergy and was not taking any medications. Because a solid cystic tumor was detected in the left thyroid lobe, we decided to perform fine-needle aspiration cytology (FNAC). When the second aspiration was performed with a 22G needle inserted into the nodule in the left lobe, the patient immediately complained of pain in her neck. Within 2 minutes, her anterior neck swelled up and ultrasonography showed vascular dilatation in the left thyroid lobe. Neck cooling with steroid injection was undertaken. However, over the next 5 minutes, the vascular dilatation also spread to the right lobe. About 15 minutes later, the vessels gradually became unclear, and the neck pain and swelling persisted, and the patient was hospitalized for observation. By the following day, all the symptoms and signs had resolved and the patient was discharged. FNA is associated with the risk of neck swelling caused by hemorrhage or dTSaFNA. To make a prompt diagnosis and provide appropriate treatment for this complication, ultrasonography could be useful.
News media representation on EU immigration before Brexit: the ‘Euro-Ripper’ case
Authors: Martins Marta 
Published: January 2021

AbstractA higher level of mobility of people has marked the European Union (EU), with immigrants moving from one place to another, every year, looking for a better quality of life, often fleeing from war and poverty. In the wake of enlargement of the European Union, the United Kingdom (UK) experienced high inward migration. One of the main focuses of UK media coverage was immigration from Eastern European countries. The UK referendum on Brexit on 23 June 2016, was followed by an increase in hate crimes linked to migration issues and, subsequently, a media apparatus of toxic discourse and fear of the criminal ‘Other’. This paper aims to reveal how newspaper articles and personal comments written in response to these articles, represented creative and media-driven anxieties about ‘opening’ borders in the EU. The empirical sample builds on news media coverage of the ‘Euro-Ripper’ case, published in two UK newspapers—the Daily Mail and The Independent. Based on critical surveillance studies and cultural media studies, I elaborate on the notion of moral panic, dramatised by the media, which mobilises specific compositions of ‘otherness’ by constructing suspicion and criminalising inequality by particular social and ethnic groups and nationalities. I argue that the media portrays the dramatisation of transnational narratives of risk and (in)security, which redraws territorial borders and (re)define Britain’s global identity. The analysis shows how the news media in the Brexit vote continually raised and legitimised awareness related to the migration as a vehicle that enables the ‘folk-devil’ to cross borders. This context postulates an ideology that converges on a relationship of intransigence and criminal convictions, in the context of a politics of inclusion and exclusion. I conclude by emphasising how the media intersects different social and geographical spaces in which migration takes place. Media-constructed categories of suspicion targets have been previously created and ‘suspect communities’ have already been socially accepted, thereby confirming and reshaping understandings of their identities and communities.
Drebrin regulates cytoskeleton dynamics in migrating neurons through interaction with CXCR4
Authors: Shan Yufei , Farmer Stephen Matthew , Wray Susan 
Published: January 2021

SignificanceProper neuronal migration is critical for the formation of correct neural circuits during development. Chemokines are known to facilitate neuronal migration. However, the underlying mechanism is still unclear. Here, we reveal a novel mechanism whereby the chemokine receptor (CXCR4), after being activated by its ligand, SDF-1, interacts directly with the cytoskeleton compartment via drebrin, an actin-binding protein that interacts with microtubule plus-end–binding proteins. The interaction between activated CXCR4/drebrin promotes neuronal migration. The study provides insights on the relationship between extracellular cues and intracellular components.
Ataluren and aminoglycosides stimulate read-through of nonsense codons by orthogonal mechanisms
Authors: Ng Martin Y. , Li Hong , Ghelfi Mikel D. , Goldman Yale E. , Cooperman Barry S. 
Published: January 2021

Significance
Nonsense mutations giving rise to premature stop codons (PSCs) cause many diseases, creating the need to develop safe and effective translational read-through–inducing drugs (TRIDs). The current best-characterized TRIDs are ataluren and aminoglycosides. Only ataluren has been approved for clinical use, albeit in a limited context. Here, we provide rate measurements of elementary steps in a single eukaryotic translation elongation cycle, allowing us to demonstrate that ataluren and the aminoglycoside G418 employ orthogonal mechanisms in stimulating PSC read-through: ataluren by inhibiting release factor-dependent termination of protein synthesis and G418 by increasing functional near-cognate transfer RNA mispairing, which permits continuation of synthesis. We conclude that development of new TRIDs combatting PSC diseases should prioritize those directed toward inhibiting termination.
Inner ear and the balance between thrombosis and bleeding
Authors: Costa Joana Raquel , Martins Hugo , Soares Teresa , Almeida e Sousa Cecília 
Published: January 2021

Introduction
This paper reports a case of spontaneous intralabyrinthine haemorrhage as a cause of sudden sensorineural hearing loss, and discussed the implications that abnormal intralabyrinthine findings may have in the treatment of these cases.

Case report
A 56-year-old woman with history of anticoagulants intake, was diagnosed with profound right-sided sensorineural hearing loss. Brain/inner ear Magnetic Resonance Imaging showed right-side labyrinthine haemorrhage. Blood tests were within the appropriate range. The patient began treatment with corticosteroids, and on the fifth day of treatment, because there was no improvement, suspend anticoagulation, for being possible and safe. The patient also started hyperbaric oxygen therapy, with mild improvement in hearing function.

Discussion
In fact, there is no consensus in literature that specifies the best management of a proven intralabyrinthine haemorrhage. Further studies with representative samples are needed in order to understand whether the suspension of anticoagulants, when possible, may have real prognostic implications, as noted in the reported case. Our case represents one of the few reported in the literature that shows the possible results of the suspension of oral anticoagulation in the recovery of the audiological and vestibular consequences of intra-labyrinthine haemorrhage.
ANALYSIS AND CHARGE CONTROL OF LITHIUM ION BATTERY WITH APPLICATION FOR OFF-GRID PV SYSTEM
Authors: Dash Smruti , Sarojini L 
Published: January 2021

Abstract
For the implementation of the PV micro grid, initially we designed the PV module. Once the PV module is designed we then decided to use P&O technique as an MPPT technique. The output pulse of the MPPT technique is then fed to the boost converter as gate pulse. The output of PV with dc to dc converter is then fed to DC grid from where the 3-phase Inverter with LC filter which converts the DC to 3-phase AC system and then is fed to load. Also the battery system with bidirectional controller is followed by a charge controller is also connected to DC micro grid so that the battery can charge or discharge as per the application.