bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term single-cell imaging and simulations of microtubules reveal principles behind wall patterning during proto-xylem development
Authors: Schneider René , Klooster Kris van’t , Picard Kelsey L. , van der Gucht Jasper , Demura Taku , Janson Marcel , Sampathkumar Arun , Deinum Eva E. , Ketelaar Tijs , Persson Staffan 
Published: January 2021

AbstractPlants are the tallest organisms on Earth; a feature sustained by solute-transporting xylem vessels in the plant vasculature. The xylem vessels are supported by strong cell walls that are assembled in intricate patterns. Cortical microtubules direct wall deposition and need to rapidly re-organize during xylem cell development. Here, we establish long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans-differentiation, resulting in spiral wall patterns, to understand microtubule re-organization. We find that the re-organization requires local microtubule de-stabilization in band-interspersing gaps. Using microtubule simulations, we recapitulate the process in silico and predict that spatio-temporal control of microtubule nucleation is critical for pattern formation, which we confirm in vivo. By combining simulations and live-cell imaging we further explain how the xylem wall-deficient and microtubule-severing KATANIN contributes to microtubule and wall patterning. Hence, by combining quantitative microscopy and modelling we devise a framework to understand how microtubule re-organization supports wall patterning.
Proliferative polyploid cells give rise to tumors via ploidy reduction
Authors: Matsumoto Tomonori , Wakefield Leslie , Peters Alexander , Peto Myron , Spellman Paul , Grompe Markus 
Published: January 2021

AbstractPolyploidy is a hallmark of cancer, and closely related to chromosomal instability involved in cancer progression. Importantly, polyploid cells also exist in some normal tissues. Polyploid hepatocytes proliferate and dynamically reduce their ploidy during liver regeneration. This raises the question whether proliferating polyploids are prone to cancer via chromosome missegregation during mitosis and/or ploidy reduction. Conversely polyploids could be resistant to tumor development due to their redundant genomes. Therefore, the tumor-initiation risk of physiologic polyploidy and ploidy reduction is still unclear. Using in vivo lineage tracing we here show that polyploid hepatocytes readily form liver tumors via frequent ploidy reduction. Polyploid hepatocytes give rise to regenerative nodules with chromosome aberrations, which are enhanced by ploidy reduction. Although polyploidy should theoretically prevent tumor suppressor loss, the high frequency of ploidy reduction negates this protection. Importantly, polyploid hepatocytes that undergo multiple rounds of cell division become predominantly mononucleated and are resistant to ploidy reduction. Our results suggest that ploidy reduction is an early step in the initiation of carcinogenesis from polyploid hepatocytes.
Genome-wide meta-analysis of muscle weakness identifies 15 susceptibility loci in older men and women
Authors: Jones Garan , Trajanoska Katerina , Santanasto Adam J. , Stringa Najada , Kuo Chia-Ling , Atkins Janice L. , Lewis Joshua R. , Duong ThuyVy , Hong Shengjun , Biggs Mary L. , Luan Jian’an , Sarnowski Chloe , Lunetta Kathryn L. , Tanaka Toshiko , Wojczynski Mary K. , Cvejkus Ryan , Nethander Maria , Ghasemi Sahar , Yang Jingyun , Zillikens M. Carola , Walter Stefan , Sicinski Kamil , Kague Erika , Ackert-Bicknell Cheryl L. , Arking Dan E. , Windham B. Gwen , Boerwinkle Eric , Grove Megan L. , Graff Misa , Spira Dominik , Demuth Ilja , van der Velde Nathalie , de Groot Lisette C. P. G. M. , Psaty Bruce M. , Odden Michelle C. , Fohner Alison E. , Langenberg Claudia , Wareham Nicholas J. , Bandinelli Stefania , van Schoor Natasja M. , Huisman Martijn , Tan Qihua , Zmuda Joseph , Mellström Dan , Karlsson Magnus , Bennett David A. , Buchman Aron S. , De Jager Philip L. , Uitterlinden Andre G. , Völker Uwe , Kocher Thomas , Teumer Alexander , Rodriguéz-Mañas Leocadio , García Francisco J. , Carnicero José A. , Herd Pamela , Bertram Lars , Ohlsson Claes , Murabito Joanne M. , Melzer David , Kuchel George A. , Ferrucci Luigi , Karasik David , Rivadeneira Fernando , Kiel Douglas P. , Pilling Luke C. 
Published: January 2021

AbstractLow muscle strength is an important heritable indicator of poor health linked to morbidity and mortality in older people. In a genome-wide association study meta-analysis of 256,523 Europeans aged 60 years and over from 22 cohorts we identify 15 loci associated with muscle weakness (European Working Group on Sarcopenia in Older People definition: n = 48,596 cases, 18.9% of total), including 12 loci not implicated in previous analyses of continuous measures of grip strength. Loci include genes reportedly involved in autoimmune disease (HLA-DQA1p = 4 × 10−17), arthritis (GDF5p = 4 × 10−13), cell cycle control and cancer protection, regulation of transcription, and others involved in the development and maintenance of the musculoskeletal system. Using Mendelian randomization we report possible overlapping causal pathways, including diabetes susceptibility, haematological parameters, and the immune system. We conclude that muscle weakness in older adults has distinct mechanisms from continuous strength, including several pathways considered to be hallmarks of ageing.
An antibonding valence band maximum enables defect-tolerant and stable GeSe photovoltaics
Authors: Liu Shun-Chang , Dai Chen-Min , Min Yimeng , Hou Yi , Proppe Andrew H. , Zhou Ying , Chen Chao , Chen Shiyou , Tang Jiang , Xue Ding-Jiang , Sargent Edward H. , Hu Jin-Song 
Published: January 2021

AbstractIn lead–halide perovskites, antibonding states at the valence band maximum (VBM)—the result of Pb 6s-I 5p coupling—enable defect-tolerant properties; however, questions surrounding stability, and a reliance on lead, remain challenges for perovskite solar cells. Here, we report that binary GeSe has a perovskite-like antibonding VBM arising from Ge 4s-Se 4p coupling; and that it exhibits similarly shallow bulk defects combined with high stability. We find that the deep defect density in bulk GeSe is ~1012 cm−3. We devise therefore a surface passivation strategy, and find that the resulting GeSe solar cells achieve a certified power conversion efficiency of 5.2%, 3.7 times higher than the best previously-reported GeSe photovoltaics. Unencapsulated devices show no efficiency loss after 12 months of storage in ambient conditions; 1100 hours under maximum power point tracking; a total ultraviolet irradiation dosage of 15 kWh m−2; and 60 thermal cycles from −40 to 85 °C.
Simulating the fabrication of aluminium oxide tunnel junctions
Authors: Cyster M. J. , Smith J. S. , Vogt N. , Opletal G. , Russo S. P. , Cole J. H. 
Published: January 2021

AbstractAluminium oxide (AlOx) tunnel junctions are important components in a range of nanoelectric devices including superconducting qubits where they can be used as Josephson junctions. While many improvements in the reproducibility and reliability of qubits have been made possible through new circuit designs, there are still knowledge gaps in the relevant materials science. A better understanding of how fabrication conditions affect the density, uniformity, and elemental composition of the oxide barrier may lead to the development of lower noise and more reliable nanoelectronics and quantum computers. In this paper, we use molecular dynamics to develop models of Al–AlOx–Al junctions by iteratively growing the structures with sequential calculations. With this approach, we can see how the surface oxide grows and changes during the oxidation simulation. Dynamic processes such as the evolution of a charge gradient across the oxide, the formation of holes in the oxide layer, and changes between amorphous and semi-crystalline phases are observed. Our results are widely in agreement with previous work including reported oxide densities, self-limiting of the oxidation, and increased crystallinity as the simulation temperature is raised. The encapsulation of the oxide with metal evaporation is also studied atom by atom. Low density regions at the metal–oxide interfaces are a common feature in the final junction structures which persists for different oxidation parameters, empirical potentials, and crystal orientations of the aluminium substrate.
Ti3+ in corundum traces crystal growth in a highly reduced magma
Authors: Oliveira Beñat , Griffin William L. , Gain Sarah E. M. , Saunders Martin , Shaw Jeremy , Toledo Vered , Afonso Juan Carlos , O’Reilly Suzanne Y. 
Published: January 2021

AbstractAggregates of corundum crystals with skeletal to hopper morphology occur in pyroclastic rocks erupted from Cretaceous basaltic volcanoes on Mt Carmel, N. Israel. The rapid growth of the crystals trapped volumes of the parental Al2O3-supersaturated melt; phenocrysts of tistarite (Ti2O3) in the trapped melts indicate crystallization at oxygen fugacities 6–7 log units below the Iron-Wüstite buffer (fO2 = ΔIW − 6 to − 7), induced by fluxes of mantle-derived CH4-H2 fluids. Cathodoluminescence images reveal growth zoning within the individual crystals of the aggregates, related to the substitution of Ti3+ in the corundum structure. Ti contents are < 0.3 wt% initially, then increase first linearly, then exponentially, toward adjacent melt pockets to reach values > 2 wt%. Numerical modelling indicates that the first skeletal crystals grew in an open system, from a moving magma. The subsequent linear increase in Ti reflects growth in a partially closed system, with decreasing porosity; the exponential increase in Ti close to melt pockets reflects closed-system growth, leading to dramatic increases in incompatible-element concentrations in the residual melts. We suggest that the corundum aggregates grew in melt/fluid conduits; diffusion modelling implies timescales of days to years before crystallization was terminated by explosive eruption. These processes probably operate in explosive volcanic systems in several tectonic settings.
A room-temperature ultrasonic hydrogen sensor based on a sensitive layer of reduced graphene oxide
Authors: Zhang Xue-Yu , Ma Ren-Hao , Li Ling-Sheng , Fan Li , Yang Yue-Tao , Zhang Shu-Yi 
Published: January 2021

AbstractIt is challenging to increase the sensitivity of a hydrogen sensor operating at room temperature due to weak sorption and tiny mass of hydrogen. In this work, an ultrasonic sensor is presented for detecting hydrogen, which is composed of a 128° YX-LiNbO3 substrate and a reduced graphene oxide (RGO) sensitive layer with a platinum catalyzer. By optimizing the depositing parameters of RGO and platinum, a considerably high sensitivity is achieved at room temperature. A frequency shift of 308.9 kHz is obtained in 100 ppm hydrogen mixed with argon, and a frequency shift of 24.4 kHz is obtained in 1000 ppm hydrogen mixed in synthetic air. It is demonstrated that in addition to strong sorption of the sensitive layer, the coaction of mass load and conductivity variation is key to high sensitivity of the sensor. By establishing the original conductivity of the sensitive layer within the “conductivity window” for enhancing electrical response, we improve the sensitivity of the ultrasonic sensor, which is available for detecting hydrogen with an extremely low concentration of 5 ppm.
Clinical manifestations of focal segmental glomerulosclerosis in Japan from the Japan Renal Biopsy Registry: age stratification and comparison with minimal change disease
Authors: Ozeki Takaya , Maruyama Shoichi , Imasawa Toshiyuki , Kawaguchi Takehiko , Kitamura Hiroshi , Kadomura Moritoshi , Katafuchi Ritsuko , Oka Kazumasa , Yokoyama Hitoshi , Sugiyama Hitoshi , Sato Hiroshi 
Published: January 2021

AbstractFocal segmental glomerulosclerosis (FSGS) is a serious condition leading to kidney failure. We aimed to investigate the clinical characteristics of FSGS and its differences compared with minimal change disease (MCD) using cross-sectional data from the Japan Renal Biopsy Registry. In Analysis 1, primary FSGS (n = 996) were stratified by age into three groups: pediatric (< 18 years), adult (18–64 years), and elderly (≥ 65 years), and clinical characteristics were compared. Clinical diagnosis of nephrotic syndrome (NS) was given to 73.5% (97/132) of the pediatric, 41.2% (256/622) of the adult, and 65.7% (159/242) of the elderly group. In Analysis 2, primary FSGS (n = 306) and MCD (n = 1303) whose clinical diagnosis was nephrotic syndrome (NS) and laboratory data were consistent with NS, were enrolled. Logistic regression analysis was conducted to elucidate the variables which can distinguish FSGS from MCD. On multivariable analysis, higher systolic blood pressure, higher serum albumin, lower eGFR, and presence of hematuria associated with FSGS. In Japanese nationwide registry, primary FSGS patients aged 18–64 years showed lower rate of NS than those in other ages. Among primary nephrotic cases, FSGS showed distinct clinical features from MCD.
Extreme miniaturization of a new amniote vertebrate and insights into the evolution of genital size in chameleons
Authors: Glaw Frank , Köhler Jörn , Hawlitschek Oliver , Ratsoavina Fanomezana M. , Rakotoarison Andolalao , Scherz Mark D. , Vences Miguel 
Published: January 2021

AbstractEvolutionary reduction of adult body size (miniaturization) has profound consequences for organismal biology and is an important subject of evolutionary research. Based on two individuals we describe a new, extremely miniaturized chameleon, which may be the world’s smallest reptile species. The male holotype ofBrookesia nanasp. nov. has a snout–vent length of 13.5 mm (total length 21.6 mm) and has large, apparently fully developed hemipenes, making it apparently the smallest mature male amniote ever recorded. The female paratype measures 19.2 mm snout–vent length (total length 28.9 mm) and a micro-CT scan revealed developing eggs in the body cavity, likewise indicating sexual maturity. The new chameleon is only known from a degraded montane rainforest in northern Madagascar and might be threatened by extinction. Molecular phylogenetic analyses place it as sister toB. karchei, the largest species in the clade of miniaturizedBrookesiaspecies, for which we resurrectEvoluticaudaAngel, 1942 as subgenus name. The genetic divergence ofB. nanasp. nov. is rather strong (9.9‒14.9% to all otherEvoluticaudaspecies in the 16S rRNA gene). A comparative study of genital length in Malagasy chameleons revealed a tendency for the smallest chameleons to have the relatively largest hemipenes, which might be a consequence of a reversed sexual size dimorphism with males substantially smaller than females in the smallest species. The miniaturized males may need larger hemipenes to enable a better mechanical fit with female genitals during copulation. Comprehensive studies of female genitalia are needed to test this hypothesis and to better understand the evolution of genitalia in reptiles.
Optically transparent and very thin structure against electromagnetic pulse (EMP) using metal mesh and saltwater for shielding windows
Authors: Phan Duy Tung , Jung Chang Won 
Published: January 2021

AbstractAn electromagnetic pulse (EMP) with high energy can damage electronic equipment instantly within a wide range of thousands of kilometers. Generally, a metal plate placed inside a thick concrete wall is used against an EMP, but it is not suitable for an EMP shielding window, which requires not only strong shielding effectiveness (SE) but also optical transparency (OT). In this paper, we propose a very thin and optically transparent structure with excellent SE for EMP shielding window application. The proposed structure consists of a saltwater layer held between two glass substrates and two metal mesh layers on the outside of the glass, with a total thickness of less than 1.5 cm. The SE and OT of the structure are above 80 dB and 45%, respectively, which not only meet the requirement of EMP shielding for military purposes but also retain the procedure of good observation. Moreover, the OT of the structure can be significantly improved using only one metal mesh film (MMF) layer, while the SE is still maintained high to satisfy the required SE for home applicants. With the major advantages of low cost, optical transparency, strong SE, and flexible performance, the proposed structure can be considered a good solution for transparent EMP shielding windows.
The impact of point defects in n-type GaN layers on thermal decomposition of InGaN/GaN QWs
Authors: Grabowski Mikolaj , Grzanka Ewa , Grzanka Szymon , Lachowski Artur , Smalc-Koziorowska Julita , Czernecki Robert , Hrytsak Roman , Moneta Joanna , Gawlik Grzegorz , Turos Andrzej , Leszczyński Mike 
Published: January 2021

AbstractThe aim of this paper is to give an experimental evidence that point defects (most probably gallium vacancies) induce decomposition of InGaN quantum wells (QWs) at high temperatures. In the experiment performed, we implanted GaN:Si/sapphire substrates with helium ions in order to introduce a high density of point defects. Then, we grew InGaN QWs on such substrates at temperature of 730 °C, what caused elimination of most (but not all) of the implantation-induced point defects expanding the crystal lattice. The InGaN QWs were almost identical to those grown on unimplanted GaN substrates. In the next step of the experiment, we annealed samples grown on unimplanted and implanted GaN at temperatures of 900 °C, 920 °C and 940 °C for half an hour. The samples were examined using Photoluminescence, X-ray Diffraction and Transmission Electron Microscopy. We found out that the decomposition of InGaN QWs started at lower temperatures for the samples grown on the implanted GaN substrates what provides a strong experimental support that point defects play important role in InGaN decomposition at high temperatures.
Dental microwear texture analysis as a tool for dietary discrimination in elasmobranchs
Authors: McLennan Laura J. , Purnell Mark A. 
Published: January 2021

AbstractAs abundant and widespread predators, elasmobranchs play influential roles in food-web dynamics of marine communities. Clearly, these trophic interactions have significant implications for fisheries management and marine conservation, yet elasmobranch diet is relatively understudied; for the majority of species little or no quantitative dietary data exist. This reflects the difficulties of direct observation of feeding and stomach contents analysis in wild elasmobranchs. Here, by quantifying the 3D surface textures that develop on tooth surfaces as a consequence of feeding, we show that tooth microwear varies with diet in elasmobranchs, providing a new tool for dietary analysis. The technique can be applied to small samples and individuals with no gut contents, and thus offers a way to reduce the impact on wild elasmobranch populations of analysing their dietary ecology, especially relevant in conservation of endangered species. Furthermore, because microwear accumulates over longer periods of time, analysis of texture overcomes the ‘snapshot bias’ of stomach contents analysis. Microwear texture analysis has the potential to be a powerful tool, complementing existing techniques such as stable isotope analysis, for dietary analysis in living and extinct elasmobranchs.
Morphological variables associated with ruptured basilar tip aneurysms
Authors: Zhang Jian , Can Anil , Lai Pui Man Rosalind , Mukundan Srinivasan , Castro Victor M. , Dligach Dmitriy , Finan Sean , Gainer Vivian S. , Shadick Nancy A. , Savova Guergana , Murphy Shawn N. , Cai Tianxi , Weiss Scott T. , Du Rose 
Published: January 2021

AbstractMorphological factors of intracranial aneurysms and the surrounding vasculature could affect aneurysm rupture risk in a location specific manner. Our goal was to identify image-based morphological parameters that correlated with ruptured basilar tip aneurysms. Three-dimensional morphological parameters obtained from CT-angiography (CTA) or digital subtraction angiography (DSA) from 200 patients with basilar tip aneurysms diagnosed at the Brigham and Women’s Hospital and Massachusetts General Hospital between 1990 and 2016 were evaluated. We examined aneurysm wall irregularity, the presence of daughter domes, hypoplastic, aplastic or fetal PCoAs, vertebral dominance, maximum height, perpendicular height, width, neck diameter, aspect and size ratio, height/width ratio, and diameters and angles of surrounding parent and daughter vessels. Univariable and multivariable statistical analyses were performed to determine statistical significance. In multivariable analysis, presence of a daughter dome, aspect ratio, and larger flow angle were significantly associated with rupture status. We also introduced two new variables, diameter size ratio and parent-daughter angle ratio, which were both significantly inversely associated with ruptured basilar tip aneurysms. Notably, multivariable analyses also showed that larger diameter size ratio was associated with higher Hunt-Hess score while smaller flow angle was associated with higher Fisher grade. These easily measurable parameters, including a new parameter that is unlikely to be affected by the formation of the aneurysm, could aid in screening strategies in high-risk patients with basilar tip aneurysms. One should note, however, that the changes in parameters related to aneurysm morphology may be secondary to aneurysm rupture rather than causal.
A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization
Authors: Uddin Md Imam , Kilburn Tyler C. , Jamal Sara Z. , Duvall Craig L. , Penn John S. 
Published: January 2021

AbstractDiabetic retinopathy, retinopathy of prematurity and retinal vein occlusion are potentially blinding conditions largely due to their respective neovascular components. The development of real-time in vivo molecular imaging methods, to assess levels of retinal neovascularization (NV), would greatly benefit patients afflicted with these conditions. mRNA hybridization techniques offer a potential method to image retinal NV. The success of these techniques hinges on the selection of a target mRNA whose tissue levels and spatial expression patterns correlate closely with disease burden. Using a model of oxygen-induced retinopathy (OIR), we previously observed dramatic increases in retinal endoglin that localized to neovascular structures (NV), directly correlating with levels of neovascular pathology. Based on these findings, we have investigated Endoglin mRNA as a potential marker for imaging retinal NV in OIR mice. Also of critical importance, is the application of innovative technologies capable of detecting mRNAs in living systems with high sensitivity and specificity. To detect and visualize endoglin mRNA in OIR mice, we have designed and synthesized a novel imaging probe composed of short-hairpin anti-sense (AS) endoglin RNA coupled to a fluorophore and black hole quencher (AS-Eng shRNA). This assembly allows highly sensitive fluorescence emission upon hybridization of the AS-Eng shRNA to cellular endoglin mRNA. The AS-Eng shRNA is further conjugated to a diacyl-lipid (AS-Eng shRNA–lipid referred to as probe). The lipid moiety binds to serum albumin facilitating enhanced systemic circulation of the probe. OIR mice received intraperitoneal injections of AS-Eng shRNA–lipid. Ex vivo imaging of their retinas revealed specific endoglin mRNA dependent fluorescence superimposed on neovascular structures. Room air mice receiving AS-Eng shRNA–lipid and OIR mice receiving a non-sense control probe showed little fluorescence activity. In addition, we found that cells in neovascular lesions labelled with endoglin mRNA dependent fluorescence, co-labelled with the macrophage/microglia-associated marker IBA1. Others have shown that cells expressing macrophage/microglia markers associate with retinal neovascular structures in proportion to disease burden. Hence we propose that our probe may be used to image and to estimate the levels of retinal neovascular disease in real-time in living systems.