bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow Manipulation and the Association with Myocardial Systolic Function During Extracorporeal Life Support
Authors: NG Pauline Yeung , MA Tammy Sin Kwan , IP April , FANG Shu , LI Andy Chak Cheung , WONG Alfred Sai Kuen , NGAI Chun Wai , CHAN Wai Ming , SIN Wai Ching 
Published: December 2021

Abstract
Background:Peripheral veno-arterial extracorporeal membrane oxygenation (V-A ECMO) is used to support circulatory failure refractory to conventional therapy. However, data on the heart-ECMO interaction at different levels of ECMO blood flow during the immediate period after ECMO initiation are sparse. We evaluated the effects of varying ECMO blood flow rate on left ventricular systolic function.Methods:Adult patients who were supported by peripheral V-A ECMO in a tertiary referral center were recruited. Serial hemodynamic and cardiac performance parameters were measured by transthoracic echocardiogram within the first 48 hours after implementation of V-A ECMO. Measurements at 100%, 120%, and 50% of target blood flow (TBF) were compared.Results:A total of 45 patients were included, 32 (71.1%) were male, and the median age was 57 (50-64) years. The main indications for V-A ECMO were myocardial infarction 25 (55.6%) and myocarditis 6 (13.3%). With a decrease in extracorporeal blood flow from 100% to 50% of TBF, mean arterial pressure dropped from 75±18 to 67±20 mmHg (p<0.001), but stroke volume increased from 15 (8-25) to 21 (13-34) mL (p<0.001), and cardiac index increased from 0.8 (0.5-1.3) to 1.2 (0.7-1.7) L/min/m2 (p<0.001). All indices of left ventricular contractility improved at 50% compared with 100% TBF: the global longitudinal strain improved from -2.8 (-5.4-0) to -4.7 (-8.2- -1.1)% (p<0.001); left ventricular ejection fraction increased from 16.8 (10.0-28.5) to 28.2 (18.0-35.5)% (p<0.001); and left ventricular outflow tract velocity time integral increased from 4.7 (2.7-7.8) to 7.7 (3.9-11.3) cm (p<0.001). The addition of echocardiographic parameters improved the discrimination of the SAVE score in predicting hospital mortality (AUROC 0.71 vs 0.58).Conclusions:In the initial period of V-A ECMO support, left ventricular systolic function quantified bedside echocardiography was inversely related to ECMO blood flow rate. The heart-ECMO interaction should be considered when determining goals of ECMO flow after initiation.
The Clinical and Prognostic Factors for Biliary Neuroendocrine Neoplasm: A Study Based on the SEER Database
Authors: Zheng Bohao , Zhang Cheng , Wan Wenze , Sun Wentao , Cheng Xi , Ni Xiaojian , Ni Xiaoling , Suo Tao , Liu Han , Shen Sheng , Liu Houbao 
Published: December 2021

Abstract
Background: In this study, we aimed at elucidating the postoperative survival and prognostic factors in patients with biliary neuroendocrine neoplasm (NEN).Methods: Cases of biliary system NEN and adenocarcinoma were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. A propensity score matching (PSM) method was used to adjust baseline differences in clinicopathological characteristics in our analysis. The Kaplan-Meier analysis was carried out for survival analysis. Results：A total of 243 patients with biliary system NEN were enrolled in this study, of which 119 patients’ lesions located in gallbladder, while the others’ located in bile duct. The postoperative overall survival of bile duct NEN is significantly longer than that of gallbladder NEN (P<0.001). For GB-NENs, surgery method (P=0.020) and lymph node metastasis (P=0.018) were identified as independent prognostic factors. In terms of AOV NENs, age (P=0.017) and lymph node metastasis (P=0.006) were identified was independent prognostic factor, while grade (P=0.002) and lymph node metastasis (P=0.036) were identified as independent prognostic factors for EBD NENs. PSM analysis indicated that patients with biliary duct NENs have better postoperative prognosis than biliary duct adenocarcinoma.Conclusions: Patients with NEN have better overall survival than patients with adenocarcinoma. Gallbladder NEN has adverse prognosis than that of biliary tract NEN. Pathological subtype, differentiation, lymph node metastasis, surgery method and lymph node resection could affect postoperative prognosis of gallbladder and biliary tract NEN.
‘It’s the Same Thing as Giving Them CPR Training’: Rural Emergency Responders Perspectives on Naloxone
Authors: Filteau Matthew , Green Brandn , Kim Frances , McBride Ki_Ai 
Published: December 2021

Abstract
Most states in the US have implemented Good Samaritan Laws (GSLs) that provide legal protections for anyone calling law enforcement and first responders trained to administer naloxone and reverse overdoses. Despite these laws, some bystanders are reluctant to call the authorities, prompting requests to increase naloxone access and administration training among lay persons. This study examines the perceptions of emergency first responders in a frontier and remote (FAR) state to understand their job responsibilities and perceptions of layperson naloxone administration training. This study includes 22 interviews with law enforcement, EMS and/or fire personnel, members of community organizations responsible for responding to opioid overdoses. The study finds widespread support for layperson naloxone training and administration throughout Montana due to rural first responders’ inability to meet the needs of residents and an overall lack of resources to address substance use. This study adds to the literature because of it focuses on first responders in a frontier and remote area (FAR) that would benefit from layperson naloxone education and administration training due to its geographic expansiveness and the area’s overall lack of resources. A harm reduction approach that trains laypeople to administer naloxone might be FAR residents’ best chance for survival after an opioid overdose.
Efﬁcacy and Safety of Radiotherapy Plus Anti-PD1 Versus Transcatheter Arterial Chemoembolization Plus Sorafenib For Advanced Hepatocellular Carcinoma: a Real-World Study
Authors: Li Jian-Xu , Deng Wen-Xiang , Huang Shi-Ting , Lin Xiao-Feng , Long Mei-Ying , Zhang Jie , Su Ting-Shi , Li Li-Qing , Pang Ya-Dan , Liang Chun-Feng , Zhou Hong-Mei , Lu Hai-Yan , Liang Shi-Xiong , Xiang Bang-De 
Published: December 2021

Abstract
Background: The combination of transcatheter arterial chemoembolization (TACE) plus sorafenib prolonged progression-free survival (PFS) and overall survival (OS) than sorafenib or TACE monotherapy for patients with hepatocellular carcinoma (HCC). This study assessed the efficacy and safety of radiotherapy (RT) plus monoclonal antibody against programmed cell death 1 (anti-PD1) versus TACE plus sorafenib for patients with advanced HCC.Methods: Patients with advanced HCC who treated with RT plus anti-PD1 and TACE plus sorafenib were enrolled. Objective response rate (ORR), PFS, disease control rate (DCR) and OS were calculated to assess the antitumor response and the treatment-related adverse events to the safety.Results: Between January 2018 to March 2021, 37 patients underwent RT plus anti-PD1 and 41 patients underwent TACE plus sorafenib. The baseline characteristics between the two groups were comparable. The ORR and DCR were significantly higher in the RT+PD1 group than the TACE plus sorafenib group according to RECIST 1.1 (54.05% vs 12.20%, P < 0.001; 70.27% vs 46.37%, P = 0.041; respectively) and according to mRECIST (56.76% vs 31.71%, P = 0.039; 70.27% vs 46.37%, P = 0.041; respectively). RT plus anti-PD1 provided significantly better PFS (HR, 0.51; 95% CI 0.30-0.86; p=0.017) than TACE plus sorafenib. Moreover, patients with RT plus anti-PD1 had significantly higher 3-, 6-, and 9-month OS rates than those with TACE plus sorafenib(97.3% vs 92.30%, P < 0.001; 91.89% vs 68.60%, P < 0.001; 75.5% vs 60.60%, P < 0.001; respectively). The median OS was more favorable 17.4 months for the RT+PD1 group and 11.9 months for the TACE plus sorafenib group. No treatment-related death was observed. Grade 3 or more treatment-related adverse events (TRAEs) occurred significantly less in patients in the RT+PD1 group than the TACE plus sorafenib group (29.7% vs 75.6%, p < 0.001), and all TRAEs were manageable.Conclusions: In this real-world study, RT plus anti-PD1 showed significantly promising efficacy and manageable safety than TACE plus sorafenib in patients with advanced HCC. Toxicities were manageable, with no unexpected safety signals. The study provides evidence on a new therapeutic method in the treatment of advanced HCC.
The Effects of Antidepressants Fluoxetine, Sertraline, and Amitriptyline on the Development of Antibiotic Resistance in Acinetobacter Baumannii
Authors: Gürpınar Suna Sibel , Kart Didem , Eryı Müjde 
Published: December 2021

Abstract
In this study, we investigated the effects of antidepressants fluoxetine, sertraline, and amitriptyline on the development of antibiotic resistance in Acinetobacter baumannii. Susceptible clinical A. baumannii isolates were exposed to fluoxetine, sertraline, amitriptyline for 30 days, respectively. After exposure, the bacteria that developed resistance to gentamicin, imipenem, colistin, and ciprofloxacin were isolated and expression levels of some antibiotic resistance genes were compared with test bacteria in initial cultures using the quantitative Reverse-Transcriptase PCR method. The data obtained were analyzed using Student's t-test method. Increases in the MIC values of test bacteria were also determined after the exposure. The number of test bacteria that developed resistance and the MIC values of some bacteria were increased with the extension of exposure time. After exposure to fluoxetine and sertraline, decreases were observed for efflux and outer membrane porin genes in isolates that developed colistin resistance, and increases were observed in isolates that developed ciprofloxacin resistance. These observations suggest that these antidepressants have similar effects on the development of resistance. While the exposure to fluoxetine didn’t result in the development of resistance to imipenem, it was observed after exposure to sertraline and amitriptyline, and a common decrease in ompA gene expression was determined in these isolates. This study is one of the preliminary investigations that demonstrates the role of non-antibiotic drugs on the development of antibiotic resistance. To the best of our knowledge, our findings report the comparative effects of selected antidepressants on the development of antibiotic resistance in A. baumanni for the first time in the literature.
Comprehensive Analysis of the Functions, Prognostic and Diagnostic Values of RNA Binding Proteins in Head and Neck Squamous Cell Carcinoma
Authors: Ke Xueping , Fu Zhen , Yang Jingjing , Yu Shijin , Yan Tingyuan , Li Xin , Li Zhongwu , Wu Xiang 
Published: December 2021

Abstract
Background: Increasing evidence has suggested that RNA binding protein (RBP) dysregulation plays an important part in tumorigenesis. Here, we sought to explore the potential molecular functions and clinical significance of RBP and develop diagnostic and prognostic signatures based on RBP in patients with head and neck squamous cell carcinoma (HNSCC). Methods: The Limma package was applied to identify the differently expressed RBPs between HNSCC and normal samples with |log2 fold change (FC)|≥1 and false discovery rate (FDR)<0.05. the immunohistochemistry images from the Human Protein Atlas database The diagnostic signature based on RBP was built by LASSO-logistic regression and random forest and the prognostic signature based on RBP was constructed by LASSO and stepwise Cox regression analysis in training cohort and validated in validation cohort. All these analyses were performed using the R software.Results: A total of 84 aberrantly expressed RBPs were obtained, comprising 41 up-regulated and 43 down-regulated RBPs. Seven RBP genes (CPEB3, PDCD4, ENDOU, PARP12, DNMT3B, IGF2BP1, EXO1) were identified as diagnostic related hub gene and were used to establish a diagnostic RBP signature risk score (DRBPS) model by the coefficients in LASSO-logistic regression analysis and shown high specificity and sensitivity in the training (area under the receiver operating characteristic curve [AUC] = 0.998), and in all validation cohorts (AUC > 0.95 for all). Similarly, seven RBP genes (MKRN3, ZC3H12D, EIF5A2, AFF3, SIDT1, RBM24 and NR0B1) were identified as prognosis associated hub genes by least absolute shrinkage and selection operator (LASSO) and stepwise multiple Cox regression analyses and were used to construct the prognostic model named as PRBPS. The area under the curve of the time-dependent receiver operator characteristic curve of the prognostic model were 0.664 at 3 years and 0.635 at 5 years in training cohort and 0.720, 0.777 in the validation cohort, showing a favorable predictive effificacy for prognosis in HNSCC.Conclusions: Our results demonstrate the values of consideration of RBP in the diagnosis and prognosis for HNSCC and provide a novel insights to understand potential role of dysregulated RBP in HNSCC.
Recursive State and Parameter Estimation of COVID-19 Circulating Variants Dynamics
Authors: Silva Daniel Martins , Secchi Argimiro Resende 
Published: December 2021

Abstract
COVID-19 pandemic response with non-pharmaceutical interventions is an intrinsic control problem. Governments balance social distancing policies to avoid overload on health system without major economic impact. A control strategy requires reliable predictions to be efficient on long-term. SARS-CoV-2 mutability, vaccination coverage and time-varying restrictive measures change virus evolution dynamics frequently. State and parameter estimations are an option do deal with these uncertainties. In this paper, a SIR-based model is proposed considering data available and feedback corrections over time. State and parameter estimations were done on state estimators with augmented states. Three observers were implemented: Constrained Extended Kalman Filter (CEKF), CEKF and Smoother (CEKF&S) and Moving Horizon Estimator (MHE). The parameters estimated therein are based on vaccine efficacy studies regarding transmissibility, severeness of disease and lethality. Social distancing is a measured disturbance calculated with Google mobility data. Six federative units from Brazil are used to evaluate proposed strategy: Amazonas, Mato Grosso do Sul, Rio Grande do Norte, Rio Grande do Sul, Rio de Janeiro and São Paulo. State and parameter estimations were realized from October 1 st 2020 to July 1 st 2021 during which Zeta and Gamma variants emerged. Results showed an efficient detection of circulating variants from proposed parameter estimation. In addition, it asserted dynamics related to virus mutations. Zeta mutations increase lethality between 19 and 45%, and increased transmissibility between 20 and 38%. Gamma mutations, on the other hand, increased lethality between 62 and 110% while increasing transmissibility between 52 and 107%. Furthermore, parameter estimation indicated existence and temporal change of subnotification on hospitalized and deceased individuals. Overall, dynamics estimated were within expectations and are applicable to control theory.
The Effects of Exercise Therapy Moderated by Sex in Rehabilitation of COVID-19
Authors: Rausch Linda Katharina , Puchner Bernhard , Fuchshuber Jürgen , Seebacher Barbara , Löffler-Ragg Judith , Pramsohler Stephan , Netzer Nikolaus C. , Faulhaber Martin 
Published: December 2021

Abstract
BackgroundPulmonary rehabilitation serves as a key component in the recovery of COVID-19 and standardized exercise therapy programs in pulmonary rehabilitation have been shown to significantly improve physical performance and lung function parameters in post-acute COVID-19 patients. However, it has not been investigated if these positive effects are equally beneficial for both sexes, especially considering a more severe physical impact of COVID-19 in men when compared to women. Therefore, the purpose of this study was to analyze outcomes of a pulmonary rehabilitation program with respect to sex differences, in order to identify sex-specific pulmonary rehabilitation requirements.MethodsData of 233 patients were analyzed before and after a three-week standardized pulmonary rehabilitation program. Patients were admitted to rehabilitation due to post-acute COVID-19 illness and staged using the COVID-19 Severity Scale by Huang et al. (2021). Lung function parameters were assessed as part of the clinical routine using spirometry (ICmax, maximal inspiratory capacity) and body plethysmography (FVC, forced vital capacity; FEV1, forced expiratory volume in the first second) and functional exercise capacity was measured by the Six-Minute Walk Test (6MWT). For the comparison of lung function and walking parameters by sex, Welch-ANOVA was used, as results of Levene's test suggested significant heteroscedasticity regarding the investigated parameters (p > 0.05). When comparing post-treatment 6MWT, FEV1 and FCV to corresponding reference values, paired t-tests were used.ResultsAt post-rehabilitation, ICmax, FVC, FEV1 and 6MWT has been improved in both sexes. Females showed a significantly smaller improvement in FEV1 and ICmax (F = 5.86, ω2 = .02; p < 0.05) than males. There was no statistically significant difference in FVC and 6MWT performance improvements between men and women. After the rehabilitation stay, females made greater progress towards reference values of 6MWT (T(231) = -3.04; p < 0.01) and FEV1 (T(231) = 2.83; p < 0.01) than males.ConclusionsSex differences in the improvement of lung function parameters seem to exist when completing a three-week pulmonary rehabilitation program and should be considered when personalizing standardized exercise therapies in pulmonary rehabilitation.Trial registrationthis study was registered in the German Clinical Trials Register (DRKS00026936) on 2021/10/19.
Tumor-Specific Catalysis-Mediated Enhanced Chemodynamic Therapy in Synergy with Mitophagy Inhibition Improves Therapeutic Efficacy in Endometrial Cancer
Authors: Gong Xiaodi , Wang Jing , Yang Linlin , Li Lijuan , Sun Xiao , Bai Jingfeng , Liu Jichang , Pu Xin , Wang Yudong 
Published: December 2021

Abstract
BackgroundChemodynamic therapy (CDT) relies on tumor microenvironment (e.g. high H2O2 level) responsive Fenton-like reactions to produce hydroxyl radicals (·OH) against tumors. However, endogenous H2O2 is insufficient for effective chemodynamic reactions.ResultsAn NAD(P)H: quinone oxidoreductase 1 (NQO1)highCatalase (CAT)low therapeutic window for the use of NQO1 bioactive drug β-lapachone (β-Lap) was firstly identified in endometrial cancer (EC). Accompanied by NADH depletion, β-Lap was catalyzed by NQO1 to produce excess H2O2 initiating oxidative stress, which selectively suppressed NQO1high EC cell proliferation, induced DNA double-strand breaks and promoted apoptosis. SiRNA-mediated NQO1 knockdown or dicoumarol rescued NQO1high EC cells from β-Lap-induced cytotoxicity. Arginine-glycine-aspartic acid (RGD)-functionalized iron-based metal organic frameworks-MOF(Fe) further promoted the conversion of accumulated H2O2 into highly oxidative ·OH, and in turn exacerbated the oxidative damage to RGD-positive target cells. Mitophagy inhibition by Mdivi-1 blocked a powerful antioxidant defense approach, ultimately ensuring the antitumor efficacy of stepwise amplified ROS signals. The tumor growth inhibition rate was about 85.92%.ConclusionsTumor specific chemotherapy in combination with CDT-triggered therapeutic modality presented unprecedented therapeutic advantages for the treatment of NQO1+ advanced type I or type II EC.
Evaluation of Plasma Nucleosome Concentrations In Healthy Dogs And Dogs With A Variety of Common Cancers.
Authors: Wilson-Robles Heather , Bygott Thomas , Kelly Theresa Kathleen , Miller Tasha , Miller Pamela , Matsushita Matthew , Terrell Jason , Bougoussa Mhammed , Butera Thomas 
Published: December 2021

Abstract
Background: Cell free DNA, in the form of nucleosomes, is released into circulation during apoptosis and necrosis in a variety of diseases. They are small fragments of chromosomes that are composed of DNA wrapped around a histone core made of four duplicate histone proteins forming an octamer. The nucleosome compartment is a relatively uninvestigated area of circulating tumor biomarkers in dogs. The objectives of this study were to quantify and better characterize nucleosomes in 528 dogs with various common malignancies and 134 healthy dogs. Results: The sensitivity of elevated circulating nucleosome concentrations for the detection of cancer in all dogs was 49.8% with a specificity of 97% with an area under the curve of 68.74%. The top 4 malignancies detected by the test included lymphoma, hemangiosarcoma, histiocytic sarcoma and malignant melanoma. The malignancies least likely to be detected were soft tissue sarcomas and mast cell tumors. Conclusions: A variety of tumor types may cause elevated nucleosome concentations in dogs. Tumors of hematopoietic origin are most likely to cause elevations and local tumors such as soft tissue sarcomas are least likely to cause elevations in plasma nucleosome concentrations.
Fluorescent Indolo[2,3-b]quinoxalin-2-yl)(Phenyl)methanone Dyes: Photophysical, Aie Activity, Electrochemical, and Theoretical Studies
Authors: Kanekar Deepali , Dhanawade Sudhakar , Jadhav Anand , Ghadiyali Mohmmed , Chacko Sajeev , Kamble Rajesh M 
Published: December 2021

Abstract
Herein, we have synthesized four indolo[2,3-b]quinoxalin-2-yl)(phenyl)methanone derivatives 1−4 by cyclocondensation. The photophysical studies of dyes in various solvents and neat solid film exhibit typical electronic spectra with inbuilt intramolecular charge transfer (ICT) (λmax: 397‒490 nm) confirming donor-acceptor architecture. Herein, dyes fluoresce in the blue-orange region (λEmax: 435–614) on excitation at their ICT maxima in toluene, ethyl acetate, chloroform, DMSO, and neat solid film. 1 and 2 which exhibit good emission intensity in all mediums, were studied for aggregation-induced emission (AIE) effect. Electrochemical studies indicate 1−4 possess relatively low lying LUMO (‒3.65 to ‒3.98 eV) comparable to reported n-type/electron-transporting materials. The HOMO and LUMO energy levels in 1−4 were evaluated by DFT and TD-DFT calculations. TGA analysis shows 1−4 exhibit good thermal stability. The characteristic optoelectronic properties and thermal stability signify these dyes are potential candidate for their application in optoelectronics.
Ultrahigh Permittivity, Ultralow Loss and Stability in (In0.5Ta0.5)0.1Ti0.9O2 Ceramics With Temperature Range From -100 to 235 °C
Authors: Xue Ying , Wang Zhuo , Li Yanxin , Yi Zhihui , Li Xin , Wu Dan 
Published: December 2021

Abstract
Dielectric materials with excellent dielectric properties are being promoted in requirements of microelectronic devices. In this study, (In0.5Ta0.5)0.1Ti0.9O2 ceramics were achieved by a solid-state reaction with reducing atmosphere of N2. Also, dense microstructure, ultrahigh permittivity (εr = 1.18 × 105) and ultralow dielectric loss (tanδ = 0.0072) were demonstrated at1 kHz. Interestingly enough, the temperature coefficient of permittivity which satisfies X9D (-100 °C - 235 °C, Δεr/ε25°C < ± 3.3 %) maintained stability at 1 kHz, and the dielectric mechanism could be connected to the electron-pinned defect dipoles (EPDD), which has favourable potential applications in electronic devices.