bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Nuclear Histomorphometry for distinguishing Undifferentiated Carcinoma from Non-Hodgkin’s Malignant Lymphoma and Benign Mimics
Authors: Hermin Aminah Usman  , Okky Husain  , Hasrayati Agustina  , Widyawardana Adiprawita  
Published: January 2025

Background: Undifferentiated carcinomas visually cannot be discriminated from Non-Hodgkin’s Malignant Lymphomas (NHMLs) and need additional immunohistochemistry (IHC) examination. Some benign lesions are also mimicking the case above. Not every anatomical pathology laboratory is capable of performing IHC exams. Quantitative Nuclear Histomorphometry (QNH) is a computer-aided image analysis of digitized pathology image analysis. The aim of this study is to measure the accuracy of QNH to distinguish undifferentiated neoplasms and their benign as a potential for triagning IHC exams.
Methods: Histopathological slides of undifferentiated carcinomas, NHMLs, and benign lesions that IHC has confirmed are collected. A series of images from selected regions of interest from Hematoxylin and Eosin (HE) slides are acquired under 100X and 400X magnifications. Hematoxylin-only images are generated from HE photomicrograph, further segmented, and its nuclear features are extracted. Features are further detailed in its five number summaries. One-vs-rest (OvR) lasso regression was performed to select relevant features. Other OvR binary logistical regressions are performed to create models for predicting diagnosis. The sensitivity, specificity, and accuracy of each prediction model are measured. Cohen’s kappa score measures interobserver reliabilities between model.
Results: A sum of 31 cases were collected. A total of 344 and 670 PNG formatted of 1376 x 1038 pixels microphotographs from 100x and 400x magnification are acquired. Up to 300 thousand objects under 400x magnification and >1 million objects under 100x magnification are extracted. Benign lesions significantly (p<0.01) higher number of objects per image compared to both carcinomas and NHMLs. A total of 20 features for 100X magnification and 23 features for 400X magnification were selected based on Lasso Regressions. At image level, the accuracy of the model prediction under 100X and 400X magnification, each are 77.6% (p<0.01) and 83.6% (p<0.01). At slide levels in all magnification, model prediction accuracy of each magnification are (100X: 71.09%, 400X: 87.1%) compared to HE diagnosis accuracy (83.9%).
Conclusion: The QNH can be used to predict and triaging undifferentiated carcinomas, NHMLs, and benign lesions before further examination. The QNH assessment also showed high reproducibility and more objective assessment in diagnosis.
Effect of low-intensity resistance training with blood flow restriction on isometric quadriceps peak torque in knee osteoarthritis
Authors: Sari Alvita , Andriana Meisy , Pawana I Putu Alit , Tinduh Damayanti , Utomo Dwikora Novembri , Melaniani Soenarnatalina 
Published: January 2025

Link of Video Abstract: https://youtu.be/hs2pfwEtKIk

Background: Knee osteoarthritis (OA) is one of the most common chronic progressive diseases impacting people worldwide. Quadriceps muscle weakness contributes to knee-OA-related functional decline. Traditional interventions aimed at increasing muscle strength in knee-OA commonly involve high-intensity resistance training but may result in joint pain due to high compressive forces. An isometric test may be better performed with a lower level of fatigue and obtain greater tolerance for knee-OA subjects due to their articular manifestations. Current recommendations for knee-OA include low-intensity resistance training (LI-RT) performed with blood flow restriction (BFR), a potential intervention for a patient who does not tolerate high loads due to lower mechanical stress. The aim of this study is to determine the effects of blood flow restriction with a low-intensity resistance training program for six weeks on quadriceps muscle strength using an isokinetic dynamometer in patients with knee OA.
Methods: Twenty-eight patients aged 50-70 years old with knee-OA in Physical Medicine and Rehabilitation Outpatient Clinic Soetomo Hospital Surabaya were randomized into the control group (CG) and intervention group (IG). Both groups underwent a 6-week supervised training program consisting of LI-RT (30% 1-RM) two sessions/week. The intervention group received an additional application of blood flow restriction on 1/3 of the upper thigh with 50 mmHg occlusion pressure. Both groups were assessed for muscle strength by isometric quadriceps peak torque using an isokinetic dynamometer before and after the protocol.
Results: Significant increases were found for isometric peak torque of quadriceps between pre and post-evaluations for LI-RT + BFR group / IG (p-value 0.000) and LI-RT group / CG (p-value 0.002). There was a significant difference in isometric peak torque improvement between the two groups (p-value 0.043). The efficiency of training time in the blood flow restriction group had a larger effect size value.
Conclusion: Low-intensity resistance training with BFR was more effective in enhancing the isometric peak torque of quadriceps compared with the low-intensity resistance training only, so it could be added as an alternative intervention for knee-OA patients.
The autoinoculation approach for treating viral warts: a literature review
Authors: Kusumaputra Bagus Haryo , Listiawan Muhammad Yulianto , Tariza Ferina Oktariani , Sari Maylita , Citrashanty Irmadita 
Published: January 2025

Link of Video Abstract: https://youtu.be/J0tXHVPgwM8

Viral warts are infections caused by the human papillomavirus (HPV) that commonly appear worldwide and at all ages. The autoinoculation treatment method of inducing the body's immune response seems to be a therapeutic option for recalcitrant viral warts. Several autoinoculation therapy techniques can be used for viral warts, which will be discussed in this review. This study aims to evaluate the autoinoculation approach for treating viral warts as a literature review. This study compiles previous eligible studies from authoritative articles published in several online databases. Autoinoculation therapy is easy to perform and does not require sophisticated equipment. It has good efficacy, where complete remission occurs in 60–75% of cases. However, there was no response in the range of 2.5 to 31% of patients. There were no harmful side effects from this treatment. Autoinoculation therapy is an inexpensive and simple technique that may be considered in multiple warts, high recurrence, and hard-to-reach areas.
Univentricular repair: a literature review
Authors: Jufri Agil Al , Soebroto Heroe , Hakim Arief Rakhman 
Published: January 2025

Link of Video Abstract: https://youtu.be/_dex85zVVSc

Univentricular repair is a surgical procedure in patients with congenital heart defects in one of the two ventricles/single ventricles (hypoplasia) or not formed (absent). The univentricular repair procedure is often called the Fontan procedure in that most of these procedures are performed in stages. There are 3 stages of this procedure which in the first stage includes a systemic-to-pulmonary shunt, pulmonary artery (PA) banding, atrial septectomy, and reconstructive procedures such as the Damus-Kaye-Stansel (DKS) and Norwood procedure; the second stage includes classic Glenn shunt, bidirectional cavopulmonary shunt (BCPS), and Hemi-Fontan surgery; and stage three includes atriopulmonary connection Fontan, extracardiac Fontan, and lateral tunnel Fontan operation. Most patients require some form of palliative therapy to regulate systemic and pulmonary arterial blood flow, such as establishing a systemic-to-pulmonary shunt (Blalock-Taussig shunt) or performing a PA bandage. Pulmonary artery reconstruction, DKS, and the Norwood procedure may be required. After 3 months, patients with a single ventricle can undergo the Bidirectional Glenn Shunt procedure, and after 18 months, these patients can undergo the Fontan procedure. Strategies for managing congenital heart disease have increased, so mortality and quality of life have also increased.
Administration of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) and Chitosan Scaffold in calvarial bone defects in Wistar rats
Authors: Sanjaya I Gusti Putu Hendra , Maliawan Sri , Sudarsa I Wayan , Mahadewa Tjokorda Gde Bagus 
Published: January 2025

Link of Video Abstract: https://youtu.be/6pIvxV9ytZs

Introduction: Autogenous bone grafting is often required in the reconstruction of extensive calvarial bone defects; however, it comes with limitations that may potentially cause patient morbidity. Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) has been widely developed for clinical use as a potent osteoblast inductors. The study aimed to prove using a chitosan scaffold combination to accelerate craniofacial bone regeneration, measured by increased osteocalcin expression, number of osteoblasts, formation of woven bone, and PDGF expression.
Method: The study used an experimental post-test-only control group design. Twenty-four male Wistar rats with calvarial bone defects were randomized into four treatment groups (control, rhBMP-2, chitosan scaffold, combination of rhBMP-2, and chitosan scaffold) and observed for four weeks. The resulting data was analyzed using an analytical Package for the Social Sciences (SPSS) software to test the hypothesis.
Result: The results showed the administration of chitosan scaffold, rhBMP-2, and a combination of rhBMP-2 and chitosan scaffold significantly increased osteocalcin expression, the number of osteoblasts, woven bone area formation, and PDGF expression compared to the control group (p<0.05). Significantly higher values (p<0.05) in all parameters were shown in the rhBMP-2 and chitosan scaffold combination group compared to those using chitosan scaffold or rhBMP-2 alone.
Conclusion: This study concluded that administering rhBMP-2 and chitosan scaffold combination accelerated the regeneration of calvarial bone defects in Wistar rats.
The immediate effect of gastrocsoleus muscle stretching with proprioceptive neuromuscular facilitation method on plantar pressure-time integral in patients with diabetes mellitus type 2
Authors: Aritonang Ricky Shubhan , Pawana I Putu Alit , Wardani Nurul Kusuma , Budi Budi 
Published: January 2025

Link of Video Abstract: https://youtu.be/5x5OcSCZI0w

Background: The purpose of this study is to look at the immediate effect of gastrocsoleus muscle stretching with the proprioceptive neuromuscular facilitation (PNF) method on plantar pressure-time integral (PTI) in patients with type 2 diabetes mellitus.
Methods: PNF stretching was used using hold relax agonist contraction (HR-AC) to stretch the gastrocsoleus muscle of seventeen diabetes mellitus patients who took part in this study. Stretching was done three times, with a 10-second hold, 6-second contraction, and 10-second hold. Hindfoot, midfoot, forefoot, big toe, and toes pressure-time integral during gait were measured and computed both before and after stretching.
Results: The pressure-time integral in the midfoot, big toe, and toes (p<0.05) was decreased, with a large effect size (r =0.53) in the big toe area. However, the pressure-time integral in the hindfoot and forefoot was increased (p>0.05).
Conclusion: PNF method stretching of gastrocsoleus muscle reduced pressure-time integral in the midfoot, big toe, and toes, and also may be an effective exercise to prevent and treat diabetic foot ulcers
Artifacts of analysis in cell line identification by short tandem repeat profiling
Authors: Malchenkova A. A. , Kosobokova E. N. 
Published: January 2025

Aim. To study and describe the most common types of artifacts in detection of short tandem repeat (STR) amplicons by capillary electrophoresis and cause difficulties in interpreting the obtained STR profiles.Material and methods. Cell lines were obtained from the bioresource collection of cell lines of the Blokhin National Medical Research Center of Oncology. DNA was isolated according to the manufacturer’s instructions of the DNeasy Blood & Tissue (QIAGEN, Germany) and ExtractDNA Blood & Cells (Evrogen, Russia) kits. DNA concentration was measured using a Qubit 4.0 device (Thermo Fisher Scientific, USA) and a Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, USA). Multiplex PCR was performed using a COrDIS EXPERT26 reagent kit (Gordiz, Russia). Capillary electrophoresis of PCR products was performed on a 3500xL Genetic Analyzer (Applied Biosystems, USA). GeneMapper Software v6.0 (Thermo Fisher Scientific, USA) was used to process electrophoresis data.Results. The most well-known artifacts associated with the STR profiling and subsequent capillary electrophoretic separation of amplicons were studied. Cases of detection of these artifacts from personal practice are given. Recommendations for improving the electrophoresis pattern are given.Conclusion. The paper studies the artifacts of analysis in cell line STR profiling by capillary electrophoresis (STR-CE), which researchers encounter in laboratory practice. Common types of analysis artifacts that cause difficulties in interpreting the results obtained during STR profiling, as well as possible reasons for their occurrence, are described in detail and illustrated with examples from our own practice. Recommendations are given for reducing the number of non-specific fluorescent signals and their intensity.
Creation of a plasma collection for the search of diagnostic biomarkers of glial tumors
Authors: Omelchuk E. P. , Timoshkina N. N. , Gvaldin D. Yu. , Petrusenko N. A. , Rostorguev E. E. , Novikova I. A. , Kit O. I. 
Published: January 2025

Aim. To create a collection of plasma samples of patients with brain tumors (BTs) for the development of a diagnostic microRNA (ribonucleic acid) panel of glial tumors.Material and methods. Plasma samples of patients with benign and malignant BTs were obtained by double centrifugation of whole blood and then frozen at -75оС.Fifty-nine RNA samples isolated from blood plasma were analyzed by next-generation sequencing (NGS).Results. Currently, the biobank contains samples from 339 patients with primary and secondary BTs and 10 control group individuals (698 samples — 2 plasma aliquots per individual), including 143 men and 206 women. The age of the patients ranged from 19 to 91 years (median — 56 years). Primary BTs (41%) included two following groups: benign (33,7%) and malignant (66,3%). Meningiomas constituted the bulk (91%) of the benign BTs. Among the malignant tumors, glioblastomas (46,7%) and astrocytomas (41,6%) prevailed, while oligodendrogliomas and ependymomas accounted for only 9,1 and 2,5%, respectively. Secondary BTs (59%) are represented by recurrent glial tumors (92,5%) and metastatic tumors (7,5%) of lung cancer (71,4%) and breast cancer (28.6%). A protocol for the primary preparation of liquid biopsy samples was implemented, which made it possible to obtain high-quality deoxyribonucleic acid libraries for the selected microRNA profiling platform.Conclusion. The creation of a plasma sample collection is the basis for searching circulating biomarkers of BTs.
Lipoprotein transport and metabolism markers in patients with myocardial infarction in the Biobank of Yugra blood sample collection
Authors: Vorobyov A. S. , Donnikov M. Yu. , Glotov O. S. , Kovalenko L. V. , Nikolaev K. Yu. , Urvantseva I. A. , Drenina Yu. A. , Morozkina A. V. , Chernysheva G. N. 
Published: January 2025

Aim. To evaluate clinical associations and predictive value of lipoprotein transport and metabolism markers determined in a collection of serum samples from patients with myocardial infarction (MI).Material and methods. Collection of blood samples from 88 patients with the acute MI was created in the Biobank of Yugra laboratory for subsequent biochemical assessment of serum levels of lipoprotein transport and metabolism markers. All patients were included in a clinical prospective study for 48 months with registration of medical events and remote tomographic assessment of coronary artery (CA) involvement upon follow-up completion.Results. Direct associations were established between proprotein convertase subtilisin/kexin type 9 (PCSK9) levels and low-density lipoprotein cholesterol levels, coronary atherosclerosis, Global Registry of Acute Coronary Events (GRACE) 2.0 risk score, and the risk of recurrent acute coronary syndrome. Lipoprotein (a) levels >50 mg/dl were detected in 28,4% of patients with MI and were associated with prior medical events, coronary and non-coronary artery atherosclerosis, comorbidity, recurrent coronary lesions, and the risk of major cardiac events at the end of follow-up. A relationship was established between a high (5,14) triglyceride-glucose index and comorbidity, recurrent coronary lesions, and the death risk at the end of follow-up.Conclusion. The Biobank of Yugra laboratory is an effective base for laboratory research. Lipoprotein transport and metabolism markers are associated with clinical factors, comorbidity, vascular atherosclerosis and a negative prognosis in patients with MI.
HEALTH PROMOTION IN IMPROVING HEALTHY LIVING BEHAVIORS IN THE COMMUNITIES OF SUCOPANGEPOK VILLAGE, JEMBER REGENCY
Authors: Wakit Saipul , Terry Ana Fauziyah  , Maylanda Osera 
Published: January 2025

Hypertension is a symptom of high blood pressure in a person. This condition causes many cases that put sufferers at risk. In Indonesia, hypertension cases are quite high and many people suffer from them. So, to overcome and prevent, practical and preventive steps are needed, including providing early education and prevention for the community to avoid the impacts caused by hypertension. These efforts are carried out through community empowerment activities through health promotion. The activity was carried out in Sukogepok village, Jember district, with the target of the activity being housewives. The aim is to identify the prevalence of people suffering from hypertension. The method used in this activity includes three stages, namely through preparation, implementation and evaluation. The implementation technique is to provide material to increase knowledge about hypertension, symptoms and efforts to prevent it. Practically, this is done through lectures, discussions, questions and answers and carrying out blood pressure checks on people who attend the activity. Blood pressure checks are carried out before and after the activity. The results show an increase in knowledge about hypertension, causes and prevention efforts. The results of the examination showed that around 35 people had high blood pressure who were categorized as hypertension.
Automatic Garlic Peeling Machine Design and Performance Evaluation
Authors: Basori Achmad , Mulyadi  
Published: January 2025

General Background the increasing demand for automation in the food processing industry requires the development of efficient technologies that can enhance productivity and ensure food safety. Specific Background the peeling of garlic is a time-consuming and labor-intensive process, often leading to contamination, thus highlighting the need for innovative solutions in garlic processing. Knowledge Gap existing peeling methods are less automated, creating a gap in the availability of efficient technology for garlic peeling that maintains hygiene standards. Aims this research aims to design and develop an automatic garlic peeler that effectively integrates mechanical, pneumatic, and electrical components to optimize the peeling process. Results experimental findings show that at a minimum pressure of 1 bar, this tool can peel an average of 28.6 grams of garlic from 812 grams, while at a maximum pressure of 5 bars, it can peel 240.6 grams. PNovelty the use of stainless steel 316 in the design provides corrosion resistance, ensuring food safety during the peeling process, which is a new approach in the context of automatic food processing technology. Implications this research contributes to advancements in food processing automation, offering insights into the integration of pneumatic systems that can enhance efficiency in culinary practices and improve hygiene standards in food handling.
Highlights:

The design integrates mechanical, pneumatic, and electrical components for optimal performance.
Peak peeling efficiency achieved at 4 bar pressure, processing 241.3 grams of garlic in 15 seconds.
Use of stainless steel 316 ensures corrosion resistance and maintains food safety standards.

Keywords: Automatic Peeler, Garlic Processing, Pneumatic System, Food Safety, Efficiency
Lead and Cadmium Levels in Urine of Sanitation Workers
Authors: Hidayah Mochamad , Hanum Galuh Ratmana 
Published: January 2025

General Background the exposure of waste management workers to heavy metals, particularly lead (Pb) and cadmium (Cd), poses significant health risks, especially among active and passive smokers. Specific Background this study aims to assess the levels of Pb and Cd in the urine of cleaning personnel working in waste disposal areas, distinguishing between active and passive smokers. Knowledge Gap limited research exists on the impact of smoking status on heavy metal accumulation in waste management workers. Aims the research focuses on determining the concentration of Pb and Cd in urine samples and understanding the potential health implications. Results findings indicate that the highest Pb level was 0.113 mg/L in passive smokers, while the lowest was 0.031 mg/L in active smokers. Novelty the study highlights the lack of correlation between Pb and Cd levels among active and passive smokers in this population. Implications these results underscore the need for regular monitoring of heavy metal exposure among waste management workers to mitigate health risks associated with environmental contaminants.
Highlights:

The highest lead concentration found in urine samples of passive smokers was 0.113 mg/L.
All measured levels of lead and cadmium were below the established safety thresholds.
No significant correlation was observed between lead and cadmium levels in active and passive smokers.

Keywords: Lead, Cadmium, Waste Management, Urine Analysis, Heavy Metals