bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concepts of responsibility in the German media debate on sugar taxation: a qualitative framing analysis
Authors: Moerschel Katharina S , von Philipsborn Peter , Hawkins Benjamin , McGill Elizabeth 
Published: November 2021

Abstract

Background
Framing plays an important role in health-policy processes. Responsibility for health is a salient and contested concept in the framing around food policies, such as sugar taxes. To deepen the understanding of the sugar tax process in Germany and contribute to a better understanding of how responsibility frames are used in debates on health policies, this study investigated responsibility concepts underlying the German media debate on sugar taxation.

Methods
We analyzed 114 national German newspaper articles, published between January 2018 and March 2019, following an inductive thematic analysis approach with an additional deductive focus on responsibility. We identified important contested concepts around sugar taxation, analyzed their combination into narrative frames, and scrutinized those narrative frames for underlying responsibility concepts.

Results
First, we identified important contested concepts regarding problems, actors and solutions (i.e. sugar tax and its potential alternatives). Those laid the basis for 13 narrative frames, of which the ‘unscrupulous industry’, ‘government failure’, ‘vulnerable youth’ and the ‘oversimplification’, ‘responsible industry’ and ‘nanny state’ frames were most salient. Within the narrative frames, we found a dominance of societal responsibility framing with a conflict between binding, legislative measures and voluntary solutions in cooperation with the food and beverages industry.

Conclusions
Questions around societal responsibility for health and corporate social responsibility framing become more salient in sugar tax debates. Future research should, therefore, investigate how public health advocates can successfully engage with corporate social responsibility narratives, and how legislative measures can be framed in ways that engender trust in governmental actions.
Development and validation of prognostic and diagnostic model for pancreatic ductal adenocarcinoma based on scRNA-seq and bulk-seq datasets
Authors: Chen Kai , Liu Xinxin , Liu Weikang , Wang Feng , Tian Xiaodong , Yang Yinmo 
Published: November 2021

Abstract
The 5-year overall survival (OS) of pancreatic ductal adenocarcinoma (PDAC) is only 10%, partly owing to the lack of reliable diagnostic and prognostic biomarkers. The raw gene-cell matrix for single-cell RNA-seq (scRNA-seq) analysis was downloaded from the GSA database. We drew cell atlas for PDAC and normal pancreatic tissues. The inferCNV analysis was used to distinguish tumor cells from normal ductal cells. We identified differential expression genes (DEGs) by comparing tumor cells and normal ductal cells. The common DEGs were used to conduct prognostic and diagnostic model using univariate and multivariate Cox or logistic regression analysis. Four genes, MET, KLK10, PSMB9 and ITGB6, were utilized to create risk score formula to predict OS and to establish diagnostic model for PDAC. Finally, we drew an easy-to-use nomogram to predict 2-year and 3-year OSs. In conclusion, we developed and validated the prognostic and diagnostic model for PDAC based on scRNA-seq and bulk-seq datasets.
Decentered? ASEAN's Struggle to Accommodate Great Power Competition
Authors: Beeson Mark 
Published: November 2021

AbstractThe Association of Southeast Asian Nations (ASEAN) currently faces a series of major, historically unprecedented, challenges. Perhaps the most consequential of these new threats is the intensifying strategic, economic, and even institutional competition between the United States and China. ASEAN's rather predictable response to this geopolitical contest has been to “hedge” and avoid choosing between the two great powers. While this strategy may be understandable, it threatens to undermine ASEAN's much vaunted “centrality” and the geopolitical and diplomatic relevance of the organization as a whole. This article explores the background to these developments and Southeast Asia's relationship with both the United States and China. I argue that the limited impact of ASEAN-style multilateralism helps to explain why great powers are creating alternative forums or simply paying lip service to the notion of ASEAN centrality.
Banking Regulatory Constraints and Personal Bankruptcy Filings in the US
Authors: Desai Chintal A , Downs David H 
Published: November 2021

ABSTRACT
The economic well-being of a household depends on its access to credit and also its access to a legal system for managing over-indebtedness. Our hypothesis is that the removal of regulatory constraints on a bank’s ability to expand in new geographic markets increases households’ access to credit, which in turn, contributes to a rise in consumer defaults. In the US, we find a net increase in Chapter 13 bankruptcies following a loosening of a state’s restrictions on multi-branch banking, compared to the increase in Chapter 13 bankruptcies in states that did not change their banking rules. The increased mortgage lending after branch deregulation helps explain this rise in Chapter 13 filings, suggesting that homeowners use the Chapter 13 code to save their houses. Further, the effects of the mortgage supply channel are greater in areas with low bank concentration. Overall, our findings are relevant to policymakers in their efforts to either set up a new personal insolvency regime or modify the existing bankruptcy process.
Long-Term Outcomes With Pharmacological Ovarian Suppression During Chemotherapy in Premenopausal Early Breast Cancer Patients
Authors: Lambertini Matteo , Boni Luca , Michelotti Andrea , Magnolfi Emanuela , Cogoni Alessio Aligi , Mosconi Anna Maria , Giordano Monica , Garrone Ornella , Arpino Grazia , Poggio Francesca , Cinacchi Paola , Bighin Claudia , Fregatti Piero , Pronzato Paolo , Blondeaux Eva , Del Mastro Lucia ,   the GIM study group
Published: November 2021

Abstract

Background
Although use of gonadotropin-releasing hormone agonist (GnRHa) during chemotherapy is an established strategy to protect ovarian function in premenopausal breast cancer patients, no long-term safety data are available, raising some concerns in women with hormone receptor–positive disease. There are controversial data on its fertility preservation potential.

Methods
The Prevention of Menopause Induced by Chemotherapy: a Study in Early Breast Cancer Patients—Gruppo Italiano Mammella 6 (PROMISE-GIM6) trial is a multicenter, randomized, open-label, phase III superiority trial conducted at 16 Italian centers from October 2003 to January 2008. Eligible patients were randomly assigned to (neo)adjuvant chemotherapy alone (control arm) or combined with the GnRHa triptorelin (GnRHa arm). The primary planned endpoint was incidence of chemotherapy-induced premature ovarian insufficiency. Post hoc endpoints were disease-free survival (DFS), overall survival (OS), and post-treatment pregnancies. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated.

Results
Of 281 randomly assigned patients, 80.4% had hormone receptor–positive breast cancer. Median follow-up was 12.4 years (interquartile range = 11.3-13.2 years). No differences in 12-year DFS (65.7% [95% CI = 57.0% to 73.1%] in the GnRHa arm vs 69.2% [95% CI = 60.3% to 76.5%] in the control arm; HR = 1.16, 95% CI = 0.76 to 1.77) or in 12-year OS (81.2% [95% CI = 73.6% to 86.8%] in the GnRHa arm vs 81.3% [95% CI = 73.1% to 87.2%] in the control arm; HR = 1.17, 95% CI = 0.67 to 2.03) were observed. In patients with hormone receptor–positive disease, the hazard ratio was 1.02 (95% CI = 0.63 to 1.63) for DFS and 1.12 (95% CI = 0.59 to 2.11) for OS. In the GnRHa and control arms, 9 and 4 patients had a posttreatment pregnancy, respectively (HR = 2.14, 95% CI = 0.66 to 6.92).

Conclusions
Final analysis of the PROMISE-GIM6 trial provides reassuring results on the safety of GnRHa use during chemotherapy as a strategy to preserve ovarian function in premenopausal patients with early breast cancer, including those with hormone receptor–positive disease.
Recurrent Gastrointestinal Bleeding in a Middle-Aged Man
Authors: Gowani Faaria , Phillips Bonnie , Leveque Christopher , Castillo Brian , Chen Jian , Chandler Wayne , Rice Lawrence , Salazar Eric 
Published: November 2021

Abstract
Acquired von Willebrand disease (avWD) arises because of mechanisms that destroy, decrease, absorb, or clear von Willebrand factor (vWF). A 59-year-old man presented with a 3-year history of recurrent gastrointestinal bleeding. Laboratory workup revealed a prolonged platelet function assay-100. The vWF antigen was decreased, and a low vWF immunofunctional activity/antigen ratio, low collagen binding/antigen ratio, and decreased intermediate and high molecular weight multimers were noted. The patient had no high-shear stress conditions, and an antibody-mediated process was suspected. A vWF mixing study showed complete correction of vWF activity, suggesting no direct functional inhibitor. The patient was given a bolus of vWF concentrate with serial measurements of vWF; the vWF half-life was 2.5 hours. The vWF propeptide/antigen ratio was 4:1, supporting a diagnosis of aVWD resulting from increased antibody-mediated vWF clearance. This case study emphasizes the laboratory’s role in the diagnosis and treatment of rare, overlooked acquired bleeding disorders.
Unique properties of spacer acquisition by the type III-A CRISPR-Cas system
Authors: Zhang Xinfu , Garrett Sandra , Graveley Brenton R , Terns Michael P 
Published: November 2021

AbstractType III CRISPR-Cas systems have a unique mode of interference, involving crRNA-guided recognition of nascent RNA and leading to DNA and RNA degradation. How type III systems acquire new CRISPR spacers is currently not well understood. Here, we characterize CRISPR spacer uptake by a type III-A system within its native host, Streptococcus thermophilus. Adaptation by the type II-A system in the same host provided a basis for comparison. Cas1 and Cas2 proteins were critical for type III adaptation but deletion of genes responsible for crRNA biogenesis or interference did not detectably change spacer uptake patterns, except those related to host counter-selection. Unlike the type II-A system, type III spacers are acquired in a PAM- and orientation-independent manner. Interestingly, certain regions of plasmids and the host genome were particularly well-sampled during type III-A, but not type II-A, spacer uptake. These regions included the single-stranded origins of rolling-circle replicating plasmids, rRNA and tRNA encoding gene clusters, promoter regions of expressed genes and 5′ UTR regions involved in transcription attenuation. These features share the potential to form DNA secondary structures, suggesting a preferred substrate for type III adaptation. Lastly, the type III-A system adapted to and protected host cells from lytic phage infection.
Chondroprotective effects of CDK4/6 inhibition via enhanced ubiquitin-dependent degradation of JUN in synovial fibroblasts
Authors: Hosoya Tadashi , Saito Tetsuya , Baba Hiroyuki , Tanaka Nao , Noda Seiji , Komiya Youji , Tagawa Yasuhiro , Yamamoto Akio , Mizoguchi Fumitaka , Kawahata Kimito , Miyasaka Nobuyuki , Kohsaka Hitoshi , Yasuda Shinsuke 
Published: November 2021

Abstract

Objective
Targeting synovial fibroblasts (SF) using a cyclin-dependent kinase (CDK) 4/6 inhibitor (CDKI) could be a potent therapy for RA via inhibition of proliferation and MMP-3 production. This study was designed to elucidate the mechanism of chondroprotective effects on SFs by CDK 4/6 inhibition.

Methods
CDK4/6 activity was inhibited using CDKI treatment or enhanced by adenoviral gene transduction. Chondroprotective effects were evaluated using a collagen-induced arthritis model (CIA). Gene and protein expression were evaluated with quantitative PCR, ELISA and Western blotting. The binding of nuclear extracts to DNA was assessed with an electrophoresis mobility shift assay. RNA-Seq was performed to identify gene sets affected by CDKI treatment.

Results
CDKI attenuated cartilage destruction and MMP-3 production in CIA. In RASFs, CDKI impaired the binding of AP-1 components to DNA and inhibited the production of MMP-1 and MMP-3, which contain the AP-1 binding sequence in their promoter. CDK4/6 protected JUN from proteasome-dependent degradation by inhibiting ubiquitination. The RNA-Seq analysis identified CDKI-sensitive inflammatory genes, which were associated with the pathway of RA-associated genes, cytokine-cytokine receptor interaction and IL-17 signalling. Notably, the AP-1 motif was enriched in these genes.

Conclusion
The mechanism of chondroprotective effects by CDK4/6 inhibition was achieved by the attenuation of AP-1 transcriptional activity via the impaired stability of JUN. Because the pharmacologic inhibition of CDK4/6 has been established as tolerable in cancer treatment, it could also be beneficial in patients with RA due to its chondroprotective and anti-inflammatory effects.
Rheumatoid arthritis patients treated with Janus kinase inhibitors show reduced humoral immune responses following BNT162b2 vaccination
Authors: Iancovici Liat , Khateeb Dina , Harel Ofer , Peri Regina , Slobodin Gleb , Hazan Yoav , Melamed Doron , Kessel Aharon , Bar-On Yotam 
Published: November 2021

Abstract

Objectives
The mRNA-based COVID-19 vaccines are now employed globally and have shown high efficacy in preventing SARS-CoV-2 infection. However, less is known about the vaccine efficacy in immune-suppressed individuals. This study sought to explore whether humoral immunity to the COVID-19 vaccine BNT162b2 is altered in RA patients treated with Janus kinase inhibitors by analysing their antibodies titre, neutralization activity and B cell responses.

Methods
We collected plasma samples from 12 RA patients who were treated with Janus kinase inhibitors and received two doses of the BNT162b2 vaccine, as well as 26 healthy individuals who were vaccinated with the same vaccine. We analysed the quantity of the anti-spike IgG and IgA antibodies that were elicited following the BNT162b2 vaccination, the plasma neutralization capacity and the responsiveness of the B-lymphocytes. We used ELISA to quantify the antibody titres, and a plasma neutralization assay was used to determine the virus neutralization capacity. Alteration in expression of the genes that are associated with B cell activation and the germinal centre response were analysed by quantitative PCR.

Results
Reduced levels of anti-spike IgG antibodies and neutralization capacity were seen in the RA patients who were treated with JAK inhibitors in comparison with healthy individuals. Furthermore, B cell responsiveness to the SARS-CoV-2 spike protein was reduced in the RA patients.

Conclusion
RA patients who are treated with JAK inhibitors show a suppressed humoral response following BNT162b2 vaccination, as revealed by the quantity and quality of the anti-spike antibodies.
Women, Mobility, and Education in Twentieth-century England and Wales: A New Analytical Approach
Authors: de Bellaigue Christina , Worth Eve , Bennett Charlotte , Eli Karin , Ulijaszek Stanley 
Published: November 2021

Abstract
The twentieth century saw substantial changes in the educational and occupational opportunities available to women in Britain. These may have been supposed to foster new patterns of female mobility. Yet studies of women’s intergenerational mobility are rare and tend not to focus on education. This article develops a historically informed gauge of educational attainment—the Educational Cohort Code (ECC). Applying that gauge to the experiences of women in twentieth-century UK, we make two key claims: first, that despite the prevalence of narratives of progress and mobility in individual and collective accounts of women’s education, there were considerable intergenerational continuities in women’s educational status across the period. Second, that the expansion of educational opportunities across the twentieth century had a differential impact for women and for men and that this differentiation destabilizes categorizations of class solely based on male occupational hierarchies. By applying the ECC method to family data, rather than focusing only on individuals, the article identifies trends within families and the possible influence of family cultures of education and employment on intergenerational mobility.
Characterizing the development of photosynthetic capacity in relation to chloroplast structure and mineral nutrition in leaves of three woody fruit species
Authors: Fu Xinyu , Zhang Jingyi , Zhou Linyao , Mo Weiping , Wang Huicong , Huang Xuming 
Published: November 2021

AbstractPlants have evolved different developmental patterns of photosynthetic capacity to better adapt to changing environmental conditions. Natural variation in photosynthetic development offers great potential for improving crop productivity. In this study, leaf developmental patterns were characterized in three woody fruit tree species with distinct photosynthetic capacity and growth habits. Changes in the photosynthetic rate, photosystem II (PSII) efficiency, chloroplast ultrastructure, activities of photosynthetic enzymes, and contents of carbohydrates and mineral nutrients were examined at five developmental stages to explore the interspecific variation in photosynthetic development. Rapid development of photosynthetic machinery and high photosynthetic capacity were found in Indian jujube (Ziziphus mauritiana) and apple (Malus domestica), whose net CO2 assimilation rate (A) peaked at full leaf expansion (FLE). Litchi (Litchi chinensis), a delayed-greening species, showed slow development of photosynthetic competence, with A peaked after FLE. The low photosynthetic capacity of litchi during early leaf expansion was associated with its delayed chloroplast development, low accumulation of starch, and low activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and NADP-glyceraldehyde-3-phosphate dehydrogenase. Correlations between mineral contents and A across leaf stages and species identified manganese as the rate-limiting nutrients in photosynthetic development in new leaves. Foliar spray of MnSO4 solution (1 g l−1) induced a short-term increase in photosynthesis in young leaves of litchi. These findings suggest that a better understanding of interspecific variation in photosynthetic development facilitates the development of new strategies for improving the photosynthetic efficiency of woody fruit trees.
How the discovery of oligochaete regeneration during the Enlightenment was pivotal to the advancement of annelid research
Authors: Rota Emilia 
Published: November 2021

Abstract
Within a few months of the year 1741, Lyonet and Trembley in Holland, Bonnet in Geneva and Réaumur in Paris all experimented industriously with the regeneration of various invertebrates, including the still unknown microdrile oligochaetes. Some of these worms were prone to spontaneous fragmentation as a natural mode of asexual multiplication, preceded (paratomy) or followed (architomy) by regenerative processes. Bonnet and Réaumur raised the stakes and began to study regeneration in earthworms, a challenge that, especially in Italy, inspired scholars and educated people among the clergy and aristocracy. Spallanzani conducted the most rigorous experiments and was the first to obtain consecutive regenerations of the head in one and the same megadrile. He saw detached mid-body sections becoming complete with a new head and a new tail, and he determined the points of the body where regeneration does not take place and how much length to leave for each section so that it could reintegrate. Above all, Spallanzani obtained regeneration of the gonadic segments, both in terrestrial and in freshwater megadriles, the latter today identified as Criodrilus lacuum. This and other important anatomical and physiological findings by Spallanzani, largely unpublished during his lifetime and for a long time after, preceded the discoveries of later authors by almost 50 years.
Effects of therapeutic plasma exchange on survival in patients with postoperative liver failure: a retrospective single-center study
Authors: Kobe Yoshiro , Tateishi Yoshihisa , Oda Shigeto 
Published: November 2021

Abstract

Background:
Recent decreases in the incidence of postoperative liver failure (POLF) have been attributed to advances in surgical techniques, critical care, and postoperative management. However, POLF management remains a challenge, and worsening POLF is a significant cause of morbidity and mortality. Therapeutic plasma exchange (TPE) is used as a salvage strategy for POLF in some countries, and several studies conducted between 1980 and 1990 have reported the use of TPE for POLF. We conducted this retrospective single-center study to investigate the outcomes of patients with POLF treated with TPE.

Methods:
We retrospectively reviewed the charts of patients with POLF admitted to the intensive care unit who underwent TPE between November 2010 and March 2017. POLF was defined as the presence of persistent hyperbilirubinemia. Data on patient demographics, Glasgow Coma Scale score, platelet count, prothrombin time, and serum total bilirubin level were collected, and their Sequential Organ Failure Assessment scores were calculated. The lengths of postoperative hospital stays, lengths of post-TPE hospital stays, and patient outcomes were also analyzed.

Results:
TPE was performed in 20 patients with POLF during the study period. TPE was initiated on the 49th postoperative day and was performed for a median of five sessions. TPE improved hyperbilirubinemia and coagulopathy but had no effect on neurological and clinical symptoms. All 20 patients treated with TPE died after the 36th day (median) from the initial TPE.

Conclusions:
Although TPE may improve laboratory values in patients with POLF, the current study suggests that it has no survival benefit.
The PIEZO1 gene mutation (c.2005G>T) causes iron overload cardiomyopathy: a case report
Authors: Cui Sumei , Lu Huixia , Wei Shujian , Li Chuanbao , Xu Feng , Chen Yuguo 
Published: November 2021

Abstract

Background:
Cardiomyopathy has a variety of etiologies. Here, we report a case of iron overload cardiomyopathy (IOC) in combination with hereditary stomatocytosis (HST) due to a rare mutation in the PIEZO1 gene.

Case summary:
A 31-year-old man presented to the clinic with a new onset of fatigue and abdominal distension. He had a history of 9-year cholelithiasis, 4-year hemolytic anemia, 7-month diabetes mellitus (DM), and 6-month low sex drive. The specific features of bronze skin, liver palms, yellow eyes, DM, and cardiomyopathy raised our suspicion of hemochromatosis, which was confirmed by an elevated serum ferritin concentration and high transferrin saturation. Echocardiography and cardiovascular magnetic resonance (CMR) imaging demonstrated dilation of all cardiac cavities with a left ventricular ejection fraction of 30%. CMR T2∗ mapping showed myocardial, hepatic, and pancreatic siderosis. Next-generation sequencing identified one missense variant in the PIEZO1 gene (c.2005G>T), which conferred HST and hyperferritinemia. We screened his close family members and identified his son as a heterozygous carrier of this variant, who had intermittent jaundice.

Conclusion:
In this case, the PIEZO1 c.2005G>T mutation conferred HST and IOC, complicated with cholelithiasis, DM, and low sex drive. Bronze skin, liver cirrhosis, cardiomyopathy, and DM are red flags, while magnetic resonance imaging T2∗ mapping, blood iron metabolism markers, and gene testing are valuable in the diagnosis.