bluuebunny/crossref_metadata_2025_split huggingface dataset:
Floral ontogeny ofDelphinium anthriscifolium(Ranunculaceae) and development of intrafloral and symmetrical resupinated organs
Authors: Zhang Wen-Gen , Liu Yu-Guang , Nie Tang-Jie , Guo Chun-Ce , Qiu Li-Hong , Yang Guang-Yao , Jabbour Florian , Wang Wei 
Published: May 2021

AbstractDelphinieae (Ranunculaceae) are characterized by zygomorphic spiral flowers and show a remarkable diversity of perianth organization. Floral structure and development have been investigated in most lineages of the tribe, mainly focusing on the establishment of symmetry and on perianth development. In this study, floral organogenesis and morphogenesis in Delphinium anthriscifolium, a member of the recently erected Delphinium subgenus Anthriscifolium, were investigated and compared with those of other species of Delphinieae in a phylogenetic context. In D. anthriscifolium, zygomorphy of the spiral flower is established soon after the initiation of sepal primordia. Among the four fully developed petals of the dorsoventralized corolla, the two dorsalmost ones become spurred, and the lateral petals resupinate before anthesis and are mirror images of each other. Resupination of petals, long overlooked in morphological studies of Delphinieae, is here reported in Ranunculaceae for the first time. The role of resupinated petals, possibly acting as a landing platform for nectar foragers, may be of high adaptive significance. Based on our new findings regarding floral ontogeny and morphology in D. anthriscifolium, we provide an updated picture of floral evolution in Delphinieae.
Ginsenoside Contents in Ginseng: Quality by Design-Coupled Two-Dimensional Liquid Chromatography Technique
Authors: Yang Yuangui , Zhang Yanhai , Zhang Xiaoqun , Yang Li , Wang Zhengtao 
Published: May 2021

Abstract
Red ginseng and white ginseng, with different chemical constituents, exhibit different antioxidative, anticancer, antiasthmatic and immunomodulatory properties. The aim of this study was to determine the amount of ginsenoside contents (Rg1, Re, Rb1, Rb2, Rc, Rd and Ro) in red and white ginseng. A rapid and comprehensive method was developed using the quality-by-design (QbD) and heart-cutting two-dimensional liquid chromatography (2D-LC) techniques. The temperature (25°C), mobile phase constituent (0.1%H3PO4), flow rate (0.35 mL/min) and concentrations of the final (45%) and initial (19.5%) organic solvents were optimized to efficient chromatography-based isolation method. The gradient program was optimized by QbD Fusion AE system. A selective column (Thermo Acclaim RSLC Polar Advantage II 2.2 μm, 100 × 2.1 mm) was used for the studies. The ginsenoside Rb1, Rc and Ro exhibiting poor separation resolution were separated using the heart-cutting 2D-LC technique. The average Rb1, Rb2 and Rc contents in red ginseng were significantly higher than the average Rb1, Rb2 and Rc contents in white ginseng. Ginsenoside Ro can be potentially used as a marker to evaluate the qualities of white and red ginseng. This comprehensive and rapid method can be potentially used to screen the quality of the markers in the future.
Acoustic Features of Cry of Deaf and Hard-of-Hearing Infants
Authors: Zayed Ahmed Mohamed , Afsah Omayma , Ismail Elshahat Ibrahem , Baz Hemmat 
Published: May 2021

Abstract
Previous research has demonstrated differences in the acoustic features of crying signals between deaf and typical hearing (TH) infants. This study aims at comparing the acoustic parameters of cries of infants with different degrees of deafness versus TH infants. About 110 infants aged 6–12 months (61 TH infants, 34 infants with bilateral deafness of variable degrees and 15 infants with unilateral deafness) were enrolled in the study. Results indicated that the most important acoustic parameters to demonstrate the difference between the crying of TH infants and deaf and hard-of-hearing (DHH) infants as well as between the crying of infants with different degrees of deafness are F0, cry duration, intensity, F2, and F4. In terms of accuracy, the parameter that showed the highest accuracy to differentiate between TH infants and DHH infants was F0 (74.5%). The accuracy of the combined acoustic parameters (F0, cry duration, intensity, and F4) was 70%. F0 was considered the best predictor and F4 the second best predictor of severity of deafness.
18F-Fluorodeoxyglucose positron emission tomography/computed tomography as a diagnostic and follow-up tool in Coxiella burnetii endocarditis of prosthetic valve and aortic valved tube: a case report
Authors: Ricciardi Elisa , Moscatelli Alessandro , Berruti Marco , Donegani Maria Isabella , Mikulska Malgorzata Karolina , Pende Aldo 
Published: May 2021

Abstract

Background
European Society of Cardiology 2015 guidelines approved 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) as a useful diagnostic imaging technique in prosthetic valve endocarditis (PVE) and recent evidence seems to suggest a role of nuclear imaging in the follow-up of cardiovascular infections, but nowadays there are no sufficient data available.

Case summary
A 67-year-old male presented with fever, weight loss, and fatigue. His medical history included ulcerative colitis and a previous Bentall-De Bono surgical procedure in 2014. A previous recent hospitalization to a small community hospital did not reveal a clear aetiology for the fever: transeosophageal echocardiography showed dubious peri-prosthetic tissue alterations, interpreted as post-surgical fibrosis; consequently, the patient was discharged with steroid therapy. At admission in our ward, we repeated transoesophageal echocardiography that confirmed the peri-prosthetic alterations. Moreover, 18F-FDG PET/CT showed two hypermetabolic areas, one around the prosthetic tube in the aortic bulb and the other in relation with the prosthetic aortic valve. Serological test was positive for Coxiella burnetii infection with consequent beginning of a targeted antimicrobial therapy with oral doxicycline and hydroxychloroquine. Echocardiography, serology, and 18F-FDG PET/CT follow-up demonstrated a progressive response to treatment and clinical conditions of the patient gradually improved.

Discussion
According to guidelines, 18F-FDG PET/CT can be used in ambiguous PVE to improve diagnostic accuracy of standard techniques. In this case, 18F-FDG PET/CT combined with echocardiography and serological tests is used not only to better define diagnosis but also for treatment response monitoring during follow-up.
IBD Systems Biology Is Here to Stay
Authors: Fiocchi Claudio , Iliopoulos Dimitrios 
Published: May 2021

Abstract

Background
Systems biology is a rapidly advancing field of science that allows us to look into disease mechanisms, patient diagnosis and stratification, and drug development in a completely new light. It is based on the utilization of unbiased computational systems free of the traditional experimental approaches based on personal choices of what is important and what select experiments should be performed to obtain the expected results.

Methods
Systems biology can be applied to inflammatory bowel disease (IBD) by learning basic concepts of omes and omics and how omics-derived “big data” can be integrated to discover the biological networks underlying highly complex diseases like IBD. Once these biological networks (interactomes) are identified, then the molecules controlling the disease network can be singled out and specific blockers developed.

Results
The field of systems biology in IBD is just emerging, and there is still limited information on how to best utilize its power to advance our understanding of Crohn disease and ulcerative colitis to develop novel therapeutic strategies. Few centers have embraced systems biology in IBD, but the creation of international consortia and large biobanks will make biosamples available to basic and clinical IBD investigators for further research studies.

Conclusions
The implementation of systems biology is indispensable and unavoidable, and the patient and medical communities will both benefit immensely from what it will offer in the near future.
Anti-TNF Therapy and the Risk of Herpes Zoster Among Patients With Inflammatory Bowel Disease
Authors: Santella Christina , Bitton Alain , Filliter Christopher , Bessissow Talat , Vutcovici Maria , Lakatos Peter L , Brassard Paul 
Published: May 2021

AbstractBackgroundThe specific contribution of anti-TNF therapy to the onset of herpes zoster (HZ) in patients with inflammatory bowel disease (IBD) remains uncertain. Thus, the purpose of this nested case-control study was to explore whether the use of anti-TNF therapy is associated with an increased risk of HZ.MethodsUsing the Regie de l’Assurance Maladie du Québec, we identified incident cases of IBD between 1998 and 2015. We matched IBD cases of HZ with up to 10 IBD HZ-free controls on year of cohort entry and follow-up. Current use was defined as a prescription for anti-TNF therapy 60 days before the index date, with nonuse as the comparator. We conducted conditional logistic regression to estimate odds ratios (ORs) with 95% confidence intervals (CIs), adjusting for potential confounders.ResultsThe cohort consisted of 15,454 incident IBD patients. Over an average follow-up of 5.0 years, 824 patients were diagnosed with HZ (incidence of 9.3 per 1000 person-years). Relative to nonuse, current use of anti-TNF therapy was associated with an overall increased risk of HZ (OR, 1.5; 95% CI, 1.1–2.1). The risk was increased among those older than 50 years (OR, 2.1; 95% CI, 1.2–3.6) and those additionally using steroids and immunosuppressants (OR, 4.1; 95% CI, 2.3–7.2).ConclusionsUse of anti-TNF therapy was associated with an increased risk of HZ among patients with IBD, particularly among those older than 50 years and those on combination therapy. Prevention strategies for HZ ought to be considered for younger IBD patients commencing treatment.
Is a hospital quality policy based on a triad of accreditation, public reporting and inspection evidence-based? A narrative review
Authors: Van Wilder Astrid , Bruyneel Luk , De Ridder Dirk , Seys Deborah , Brouwers Jonas , Claessens Fien , Cox Bianca , Vanhaecht Kris 
Published: May 2021

Abstract

Background
Since 2009, hospital quality policy in Flanders, Belgium, is built around a quality-of-care triad, which encompasses accreditation, public reporting (PR) and inspection. Policy makers are currently reflecting on the added value of this triad.

Methods
We performed a narrative review of the literature published between 2009 and 2020 to examine the evidence base of the impact accreditation, PR and inspection, both individually and combined, has on patient processes and outcomes. The following patient outcomes were examined: mortality, length of stay, readmissions, patient satisfaction, adverse outcomes, failure to rescue, adherence to process measures and risk aversion. The impact of accreditation, PR and inspection on these outcomes was evaluated as either positive, neutral (i.e. no impact observed or mixed results reported) or negative.

Objectives
To assess the current evidence base on the impact of accreditation, PR and inspection on patient processes and outcomes.

Results
We identified 69 studies, of which 40 were on accreditation, 24 on PR, three on inspection and two on accreditation and PR concomitantly. Identified studies reported primarily low-level evidence (level IV, n = 53) and were heterogeneous in terms of implemented programmes and patient populations (often narrow in PR research). Overall, a neutral categorization was determined in 30 articles for accreditation, 23 for PR and four for inspection. Ten of these recounted mixed results. For accreditation, a high number (n = 12) of positive research on adherence to process measures was discovered.

Conclusion
The individual impact of accreditation, PR and inspection, the core of Flemish hospital quality, was found to be limited on patient outcomes. Future studies should investigate the combined effect of multiple quality improvement strategies.
Transcriptome Analysis ofAedes albopictus(Diptera: Culicidae) Larvae Exposed With a Sublethal Dose of Haedoxan A
Authors: Hao Huanhuan , Zuo Yayun , Fang Jiameng , Sun Anqi , Aioub Ahmed A A , Hu Zhaonong 
Published: May 2021

AbstractAedes albopictus is the vector of arbovirus diseases including yellow fever, dengue, Zika virus, and chikungunya fever, and it poses an enormous threat to human health worldwide. Previous studies have revealed that haedoxan A (HA), which is an insecticidal sesquilignan from Phryma leptostachya L., is a highly effective natural insecticide for managing mosquitoes and houseflies; however, the mechanisms underlying the response of Ae. albopictus after treatment with sublethal concentrations of HA is not clear. Here, high-throughput sequencing was used to analyze the gene expression changes in Ae. albopictus larvae after treatment with the LC30 of HA. In total, 416 differentially expressed genes (DEGs) were identified, including 328 upregulated genes and 88 downregulated genes. Identification and verification of related DEGs were performed by RT-qPCR. The results showed that two P450 unigenes (CYP4C21 and CYP304A1), one carboxylesterase, and one ABC transporter (ABCG1) were induced by HA, which indicated that these detoxifying enzyme genes might play a major role in the metabolic and detoxification processes of HA. Additionally, acetylcholine receptor subunit ɑ2 (AChRα2), AChRα5, AChRα9, and the glutamate receptor ionotropic kainate 2 (GRIK2) were found to be upregulated in HA-treated larvae, suggesting that HA affected the conduction of action potentials and synaptic transmission by disrupting the function of neural receptors. These results provide a foundation for further elucidating the target of HA and the mechanism of detoxification metabolism in Ae. albopictus.
Nonlinear free vibration of size-dependent microbeams with nonlinear elasticity under various boundary conditions
Authors: Lin F , Peng J S , Xue S F , Yang L , Yang J 
Published: May 2021

Abstract
In this study, nonlinear couple stress–strain constitutive relationships in the modified couple stress theory (MCST) are derived on the basis of previous classical stress–strain constitutive relationships of nonlinear elasticity materials. Hamilton's principle is employed to obtain higher-order nonlinear governing equations within the framework of the updated MCST, von Kármán geometric nonlinearity and Bernoulli–Euler beam theory. These mathematical formulations are solved numerically by the differential quadrature method together with an iterative algorithm to determine the nonlinear dynamic features of microbeams with four groups of boundary conditions. A detailed parametric study is conducted to analyze the influences of nonlinear elasticity properties on the nonlinear free vibration characteristics of the microbeams. Results show that these microbeams exhibiting nonlinear couple constitutive relationships have lower frequencies than their approximately simplified linear couple constitutive relationships. In addition, the frequencies of microbeams with nonlinear elasticity properties decrease as the vibration amplitude increases.
Remimazolam alleviates neuropathic pain via regulating bradykinin receptor B1 and autophagy
Authors: Xie Haiyu , Lu Feng , Liu Weilian , Wang Enfu , Wang Lifeng , Zhong Maolin 
Published: May 2021

Abstract

Objectives
Neuropathic pain (NP) represents a broad scope of various pathological ramifications of the nervous system. Remimazolam is a proved sedative in treating neuropathic pain. Considering the Bradykinin receptor’s vital role and the potentials of Bradykinin receptor B1 (BDKRB1) in the neuropathic pain-signalling pathway, we nominated them as a primary target for remimazolam.

Methods
In this study, rats were injected with complete freund’s adjuvant (CFA) to construct NP models in vivo. BV2 microglia cells were treated with LPS to establish NP model in vitro. qRT-PCR, ELISA, western blot and immunofluorescence were applied to determine gene expression.

Key findings
Our findings revealed that BDKRB1 was overexpressed in NP models in vivo, while R715 (an antagonist of BDKRB1) suppressed the levels of BDKRB1 and inhibited the hyperpathia induced by spinal nerve litigation surgery. Moreover, remimazolam inactivated BDKRB1 signalling via suppressing NF-κB translocation and decreased the release of pro-inflammatory cytokines. Additionally, remimazolam suppressed the translocation of NF-κB, and inhibited autophagic lysosome formation in vivo and in vitro. However, R838 (an agonist of BDKRB1) reversed the effects of remimazolam.

Conclusions
Remimazolam downregulated BDKRB1, inhibited BDKRB1/RAS/MEK signalling pathway and regulated the autophagic lysosome induction, exhibiting a better outcome in the NP.
A novel survey for young substellar objects with the W-band filter III: Searching for very low-mass brown dwarfs in Serpens South and Serpens Core
Authors: Dubber Sophie , Biller Beth , Allers Katelyn , Jose Jessy , Albert Loïc , Pantoja Blake , Fontanive Clémence , Liu Michael , Zhang Zhoujian , Chen Wen-Ping , Lalchand Bhavana , Damian Belinda , Sharma Tanvi 
Published: May 2021

ABSTRACT
We present CFHT photometry and IRTF spectroscopy of low-mass candidate members of Serpens South and Serpens Core (∼430 pc, ∼0.5 Myr), identified using a novel combination of photometric filters, known as the W-band method. We report SC182952+011618, SS182959-020335, and SS183032-021028 as young, low-mass Serpens candidate members, with spectral types in the range M7-M8, M5-L0, and M5-M6.5, respectively. Best-fitting effective temperatures and luminosities imply masses of &lt; 0.12M⊙ for all three candidate cluster members. We also present Hubble Space Telescope imaging data (F127M, F139M, and F850LP) for six targets in Serpens South. We report the discovery of the binary system SS183044-020918AB. The binary components are separated by ≈45 AU, with spectral types of M7-M8 and M8-M9, and masses of 0.08–0.1 and 0.05–0.07 M⊙. We discuss the effects of high dust attenuation on the reliability of our analysis, as well as the presence of reddened background stars in our photometric sample.
Accuracy versus complexity: calibrating radio interferometer arrays with non-homogeneous element patterns
Authors: Jones Jake L , Wayth Randall B 
Published: May 2021

ABSTRACT
Radio interferometer arrays with non-homogeneous element patterns are more difficult to calibrate compared to the more common homogeneous array. In particular, the non-homogeneity of the patterns has significant implications on the computational tractability of evaluating the calibration solutions. We apply the A-stacking technique to this problem and explore the trade-off to be made between the calibration accuracy and computational complexity. Through simulations, we show that this technique can be favourably applied in the context of an SKA-Low station. We show that the minimum accuracy requirements can be met at a significantly reduced computational cost, and this cost can be reduced even further if the station calibration time-scale is relaxed from 10 min to several hours. We demonstrate the impact antenna designs with differing levels of non-homogeneity have on the overall computational complexity in addition to some cases where calibration performs poorly.
Three-dimensional structure of a flavivirus dumbbell RNA reveals molecular details of an RNA regulator of replication
Authors: Akiyama Benjamin M , Graham Monica E , O′Donoghue Zoe , Beckham J David , Kieft Jeffrey S 
Published: May 2021

AbstractMosquito-borne flaviviruses (MBFVs) including dengue, West Nile, yellow fever, and Zika viruses have an RNA genome encoding one open reading frame flanked by 5′ and 3′ untranslated regions (UTRs). The 3′ UTRs of MBFVs contain regions of high sequence conservation in structured RNA elements known as dumbbells (DBs). DBs regulate translation and replication of the viral RNA genome, functions proposed to depend on the formation of an RNA pseudoknot. To understand how DB structure provides this function, we solved the x-ray crystal structure of the Donggang virus DB to 2.1Å resolution and used structural modeling to reveal the details of its three-dimensional fold. The structure confirmed the predicted pseudoknot and molecular modeling revealed how conserved sequences form a four-way junction that appears to stabilize the pseudoknot. Single-molecule FRET suggests that the DB pseudoknot is a stable element that can regulate the switch between translation and replication during the viral lifecycle by modulating long-range RNA conformational changes.
Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase
Authors: Marsin Stéphanie , Adam Yazid , Cargemel Claire , Andreani Jessica , Baconnais Sonia , Legrand Pierre , Li de la Sierra-Gallay Ines , Humbert Adeline , Aumont-Nicaise Magali , Velours Christophe , Ochsenbein Françoise , Durand Dominique , Le Cam Eric , Walbott Hélène , Possoz Christophe , Quevillon-Cheruel Sophie , Ferat Jean-Luc 
Published: May 2021

Abstract
Replicative helicases are essential proteins that unwind DNA in front of replication forks. Their loading depends on accessory proteins and in bacteria, DnaC and DnaI are well characterized loaders. However, most bacteria do not express either of these two proteins. Instead, they are proposed to rely on DciA, an ancestral protein unrelated to DnaC/I. While the DciA structure from Vibrio cholerae shares no homology with DnaC, it reveals similarities with DnaA and DnaX, two proteins involved during replication initiation. As other bacterial replicative helicases, VcDnaB adopts a toroid-shaped homo-hexameric structure, but with a slightly open dynamic conformation in the free state. We show that VcDnaB can load itself on DNA in vitro and that VcDciA stimulates this function, resulting in an increased DNA unwinding. VcDciA interacts with VcDnaB with a 3/6 stoichiometry and we show that a determinant residue, which discriminates DciA- and DnaC/I-helicases, is critical in vivo. Our work is the first step toward the understanding of the ancestral mode of loading of bacterial replicative helicases on DNA. It sheds light on the strategy employed by phage helicase loaders to hijack bacterial replicative helicases and may explain the recurrent domestication of dnaC/I through evolution in bacteria.
Identification of combinations of somatic mutations that predict cancer survival and immunotherapy benefit
Authors: Gussow Ayal B , Koonin Eugene V , Auslander Noam 
Published: May 2021

Abstract
Cancer evolves through the accumulation of somatic mutations over time. Although several methods have been developed to characterize mutational processes in cancers, these have not been specifically designed to identify mutational patterns that predict patient prognosis. Here we present CLICnet, a method that utilizes mutational data to cluster patients by survival rate. CLICnet employs Restricted Boltzmann Machines, a type of generative neural network, which allows for the capture of complex mutational patterns associated with patient survival in different cancer types. For some cancer types, clustering produced by CLICnet also predicts benefit from anti-PD1 immune checkpoint blockade therapy, whereas for other cancer types, the mutational processes associated with survival are different from those associated with the improved anti-PD1 survival benefit. Thus, CLICnet has the ability to systematically identify and catalogue combinations of mutations that predict cancer survival, unveiling intricate associations between mutations, survival, and immunotherapy benefit.