bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein acetylation regulates xylose metabolism during adaptation of Saccharomyces cerevisiae
Authors: Tan Yong-Shui , Wang Li , Wang Ying-Ying , He Qi-En , Zhu Zhen , Song Kai , Liu Zhi-Hua , Li Bing-Zhi , Yuan Ying-Jin 
Published: September 2021

Abstract
Background
Lignocellulosic biomass upgrading has become a promising alternative route to produce transportation fuels in response to energy security and environmental concerns. As the second most abundant polysaccharide in nature, hemicellulose mainly containing xylose is an important carbon source that can be used for the bioconversion to fuels and chemicals. However, the adaptation phenomena could appear and influence the bioconversion performance of xylose when Saccharomyces cerevisiae strain was transferred from the glucose to the xylose environment. Therefore, it is crucial to elucidate the mechanism of this adaptation phenomena, which can guide the strategy exploration to improve the efficiency of xylose utilization.
Results
In this study, xylose-utilizing strains had been constructed to effectively consume xylose. It is found that the second incubation of yYST218 strain in synthetic complete-xylose medium resulted in a 1.24-fold increase in xylose consumption ability as compared with the first incubation in synthetic complete-xylose medium. The results clearly showed that growing S. cerevisiae again in synthetic complete-xylose medium can significantly reduce the stagnation time and thus achieved a faster growth rate, by comparing the growth status of the strain in synthetic complete-xylose medium for the first and second time at the single-cell level through Microfluidic technology. Although these xylose-utilizing strains possessed different xylose metabolism pathways, they exhibited the “transient memory” phenomenon of xylose metabolism after changing the culture environment to synthetic complete-xylose medium, which named ‘xylose consumption memory (XCM)’ of S. cerevisiae in this study. According to the identification of protein acetylation, partial least squares analysis and the confirmatory test had verified that H4K5Ac affected the state of “XCM” in S. cerevisiae. Knockout of the acetylase-encoding genes GCN5 and HPA2 enhanced the “XCM” of the strain. Protein acetylation analysis suggested that xylose induced perturbation in S. cerevisiae stimulated the rapid adaptation of strains to xylose environment by regulating the level of acetylation.
Conclusions
All these results indicated protein acetylation modification is an important aspect that protein acetylation regulated the state of “XCM” in S. cerevisiae and thus determine the environmental adaptation of S. cerevisiae. Systematically exploiting the regulation approach of protein acetylation in S. cerevisiae could provide valuable insights into the adaptation phenomena of microorganisms in complex industrial environments.
Aging-related variation of cuticular hydrocarbons in wild type and variant Drosophila melanogaster
Authors: Cortot Jérôme , Farine Jean-Pierre , Ferveur Jean-François , Everaerts Claude 
Published: September 2021

Abstract
The cuticle of all insects is covered with hydrocarbons which have multiple functions. Cuticular hydrocarbons (CHCs) basically serve to protect insects against environmental harm and to reduce dehydration. In many species, some CHCs also act as pheromones. CHCs have been intensively studied in Drosophila species and more specially in D. melanogaster. In this species, flies produce about 40 CHCs forming a complex sex- and species-specific bouquet. The quantitative and qualitative pattern of the CHC bouquet was characterized during the first days of adult life but remains unexplored in aging flies. Here, we characterized CHCs during the whole—or a large period of—adult life in males and females of several wild type and transgenic lines. Both types of lines included standard and variant CHC profiles. Some of the genotypes tested here showed very dramatic and unexpected aging-related variation based on their early days profile. This study provides a concrete dataset to better understand the mechanisms underlying the establishment and maintenance of CHCs on the fly cuticle. It could be useful to determine physiological parameters, including age and response to climate variation, in insects collected in the wild.
Semen parameters in men recovered from COVID-19: a systematic review and meta-analysis
Authors: Tiwari Sagar , KC Niranjan , Thapa Sajan , Ghimire Anuja , Bijukchhe Sanjeev M , Sah Guru Sharan , Isnuwardana Ronny 
Published: September 2021

Abstract
Background: The novel beta-coronavirus disease (COVID-19) has infected millions of people globally with high risk among male then female. However, the effect of COVID-19 andrology is still a subject of dispute. We planned to analyze the overall consequences of COVID-19 on semen parameters and male sex hormones.Main text: Systematic search was performed on MEDLINE and Scopus database until June 11 2021. We included observational studies, which reported mean ± standard deviation of the semen parameters and serum sex hormones of those reproductive-aged male recovered from COVID-19 and controls who did not suffered from COVID-19. We used Random-effect model to pool the studies, as heterogeneity was present. Heterogeneity was evaluated by Q test and I2. All studies were assessed with their quality and publication bias.We assessed 966 articles for eligibility and found 7 eligible studies meeting PICO criteria. This include 934 participants with mean age 37.34 ± 10.5. Random-effect model meta-analysis showed that men recovered form COVID-19 had semen parameters less than those who had not suffered from COVID-19. The overall mean difference (MD) [95% confidence interval (CI)] in semen volume, sperm concentration, sperm number, and progressive sperm motility was -0.20 (-0.45, 0.05), -16.59 (-34.82, 1.65), -45.44 (-84.56, -6.31), -1.73(-8.20, 4.75) respectively. Considering sex hormones; Luteinizing hormone and prolactin was found more among those recovered with the significant MD (95% CI) of 3.47 (1.59, 5.35) and 3.21 (1.71, 4.72) respectively.Conclusion: Both the semen parameters and sexual hormones were found to be affected after infected with COVID-19. However, the mechanism for testicular involvement remains doubtful.This systematic review and meta-analysis has been registered in PROSPERO (ID: CRD42021259445)
Pilimiction, A Rare Manifestation of Ovarian Teratoma: A Case Report
Authors: Issack Feysel Hassen , Hassen Seid Mohammed , Hassen Seid Kedir , Gebreselassie Kaleab Habtemichael , Mummed Ferid Ousman , Hassen Ibsa Kedir 
Published: September 2021

Abstract
Introduction: Adnexal teratoma involving the urinary bladder is a very rare condition. Presentation is variable ranging from irritative LUTS (lower urinary tract symptoms) to pilimiction or trichiuria (passage of hair in the urine).Case presentation: We report a case of 42-year-old woman who presented with pilimiction and lower abdominal pain. Contrast enhanced computed tomography scan (CECT) and Cystoscopy were used for the diagnosis. Tumor markers were negative. Right side salpingo-oophorectomy and partial bladder wall excision were performed. Histopathology of the specimen showed features consistent with mature teratoma. The Patient reported improvement of symptoms in the subsequent follow up visits.Conclusion: Pilimiction is a pathognomonic sign of bladder teratomas. Therefore, it is wise to think of this pathology in patients who report passage of hair through the urine (trichiuria or pilimiction), as in our case. Cystoscopy and cross-sectional imaging aided in the initial diagnosis. However, definitive diagnosis was provided by histopathology.
Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study
Authors: Fendler Annika , Au Lewis , Shepherd Scott , Byrne Fiona , Cerrone Maddalena , Boos Laura , Rzeniewicz Karolina , Gordon William , Shum Ben , Gerard Camille , Ward Barry , Xie Wenyi , Schmitt Andreas , Joharatnam-Hogan Nalinie , Cornish Georgina , Pule Martin , Mekkaoui Leila , Ng Kevin , Carlyle Eleanor , Edmonds Kim , Rosario Lyra Del , Sarker Sarah , Lingard Karla , Mangwende Mary , Holt Lucy , Ahmod Hamid , Stone Richard , Gomes Camila , Flynn Helen , Agua-Doce Ana , Hobson Philip , Caidan Simon , Howell Michael , Wu Mary , Goldstone Robert , Crawford Margaret , Cubitt Laura , Patel Harshil , Gavrielides Mike , Nye Emma , Snijders Ambrosius , MacRae James , Nicod Jerome , Gronthoud Firza , Shea Robyn , Messiou Christina , Cunningham David , Chau Ian , Starling Naureen , Turner Nicholas , Welsh Liam , As Nicholas van , Jones Robin , Droney Joanne , Banerjee Susana , Tatham Kate , Jhanji Shaman , O'Brien Mary , Curtis Oliva , Harrington Kevin , Bhide Shreerang , Bazin Jessica , Robinson Anna , Stephenson Clemency , Slattery Tim , Khan Yasir , Tippu Zayd , Leslie Isla , Gennatas Spyridon , Okines Alicia , Reid Alison , Young Kate , Furness Andrew , Pickering Lisa , Gandhi Sonia , Gamblin Steve , Swanton Charles , Nicholson Emma , Kumar Sacheen , Yousaf Nadia , Wilkinson Katalin , Swerdlow Anthony , Harvey Ruth , Kassiotis George , Larkin James , Wilkinson Robert , Turajlic Samra 
Published: September 2021

Abstract
Patients with cancer have higher COVID-19 morbidity and mortality. Here we present the prospective CAPTURE study (NCT03226886) integrating longitudinal immune profiling with clinical annotation. Of 357 patients with cancer, 118 were SARS-CoV-2-positive, 94 were symptomatic and 2 patients died of COVID-19. In this cohort, 83% patients had S1-reactive antibodies, 82% had neutralizing antibodies against WT, whereas neutralizing antibody titers (NAbT) against the Alpha, Beta, and Delta variants were substantially reduced. Whereas S1-reactive antibody levels decreased in 13% of patients, NAbT remained stable up to 329 days. Patients also had detectable SARS-CoV-2-specific T cells and CD4+ responses correlating with S1-reactive antibody levels, although patients with hematological malignancies had impaired immune responses that were disease and treatment-specific, but presented compensatory cellular responses, further supported by clinical. Overall, these findings advance the understanding of the nature and duration of immune response to SARS-CoV-2 in patients with cancer.
Investigating Fracture Failure in Origami-based Sheet Metal Bending
Authors: Ablat Muhammad Ali , Alafaghani Ala’aldin , Sun Jian-Qiao , Nikhare Chetan , Qattawi Ala 
Published: September 2021

Abstract
Origami-based sheet metal (OSM) bending is a promising new die-free folding technique for sheet metal. OSM bending principle is based on deforming the material along a pre-defined fold line, which is determined using material discontinuity (MD) produced by laser or waterjet cutting. The objective of this work is to study and evaluate the fracture in OSM bending under the influence of various MD types, kerf-to-thickness (k/t) ratios, and sheet thicknesses. The research goal is to provide information on selecting an optimized k/t ratio and type of MD that allows for fracture-free bending. Four different ductile fracture criteria (DFC) are used and calibrated from experimental data to forecast fracture. The DFC calibration is used to produce a set of critical damage values (CDV) for assessing the possibility of fracture in the OSM bending. In addition, the study provides fracture evaluation using finite element analysis (FEA) integrated with experimental cases for a broader range of OSM bending parameters and MDs. The results demonstrated that an MD with a higher k/t ratio is less likely to fracture during the OSM bending, whereas a higher sheet thickness increases the possibility of fracture. Furthermore, the study identifies the k/t ratio limit that ensures successful bending without fracture and categorizes MD types into two groups based on fracture likelihood. The fracture in the first group is dependent on the limiting k/t ratio, whereas the possibility of fracture in the second group is independent of the k/t ratio due to its topology.
Comorbid patient with chronic obstructive pulmonary disease and coronary heart disease: opportunities for early diagnosis of pulmonary hypertension in outpatient settings
Authors: Kalashnik D. N. , Korolchuk I. S. 
Published: September 2021

Introduction. A comorbid patient with the chronic obstructive pulmonary disease (COPD) in combination with cardiovascular diseases (CHD) has a poor prognosis due to the early progression of the pulmonary hypertension (PH). The study surveyed an opportunity of the PH detection using an active PH verification strategy in outpatients with COPD and stable angina pectoris.Goal. To evaluate the frequency of РH in patients with mild and moderate COPD in combination with CHD and the possibility of using the echocardiographic criterion «right atrial area» to prove РH.Materials and methods. The study included 52 outpatient patients with an average age of 62.8 ± 8.14 years. A comprehensive assessment of the Borg dyspnea scale, echocardiography, pulse oximetry at rest and after the 6-minute walk test (6MWT) were carried out. Two groups of patients were compared depending on the development of РH after T6MX.Results and discussion. It was shown that in patients with COPD (GOLD I-II) and angina pectoris, PH was initially detected in 3.3% of cases, and after the 6MWT in 63.3% of patients. In this group, after T6MX, an increase in pulmonary artery pressure was determined from 18.5 ± 10.6 mmHg to 41.2 ± 12.5 mmHg (p < 0.05). After physical activity with increased pressure in the pulmonary artery, there was a significant increase in the area of the right atrium. Only 1/3 of patients with PH had hypoxemia after the 6MWT.Conclusions. The T6M test makes it possible to detect РH in more than half of patients with COPD (GOLD I-II) and CHD in an outpatient setting. An enlargement in the area of the right atrium according to echocardiography, along with the other indicators of morphological and functional changes in the right heart, can be an additional diagnostic criterion for PH in comorbid patients with COPD and cardiovascular diseases.
Analysis of the thyroid status of pregnant women in the region of mild iodine deficiency
Authors: Suplotova L. A. , Makarova O. B. , Maksyukova E. N. , Smolina L. N. 
Published: September 2021

Introduction. Thyroid hormones play an important role during pregnancy, providing organogenesis, growth and development of the fetus, participate in the formation and development of the brain and its structures, affecting cognitive abilities. Thyroid dysfunction is associated with the pathological course of pregnancy and childbirth, as well as a negative effect on the fetus. Iodine deficiency, which persists throughout Russia, creates the preconditions for the more frequent development of subclinical hypothyroidism and hypothyroxinemia in pregnant women. Therefore, early detection of thyroid dysfunction during gestation in the region of iodine deficiency is relevant.Aim. To assess the thyroid status in pregnant women in the region of iodine deficiency.Materials and methods. A single-center, prospective, one-sample study was performed in a population of pregnant women registered from June 2019 to December 2019 to the antenatal clinic in Tyumen.Results. According to the results of biomedical monitoring of iodine deficiency in the population of pregnant women in Tyumen, iodine supply improved, as evidenced by the median concentration of ioduria 154.4 μg / l, the frequency of endemic goiter was 0.37%. Continuous screening of women in the 1st trimester of pregnancy without thyroid pathology revealed subclinical hypothyroidism in 21.5% of pregnant women, carriage of antibodies to thyroperoxidase was found in 10.5% of women.Conclusions. Given the persisting problem of iodine deficiency, the widespread occurrence of subclinical hypothyroidism in the population of pregnant women, it is necessary to include the study of thyroid status in the standard of examination of pregnant women when registering in an antenatal clinic for early diagnosis and timely treatment of hypothyroidism. It is also recommended to continue preventive measures aimed at replenishing the iodine deficiency starting from the stage of pregravid preparation.
Assessment of vitamin D supply at different age
Authors: Kondratyeva E. I. , Loshkova E. V. , Zakharova I. N. , Shubina Yu. F. , Saprina T. V. , Nikonova V. S. , Melyanovskaya Yu. L. , Zodbinova A. E. , Zhekayte E. K. , Odinaeva N. D. 
Published: September 2021

Introduction. Carrying out studies on the supply of vitamin D in the Russian population is an important task in order to demonstrate the global nature of the problem of vitamin D deficiency.Objective of the study. To conduct a study of the level of vitamin D at different age periods and to assess the relationship of the 25(OH)D supply with the seasons of the year.Materials and methods. The study included 10707 people, 78.8% (n = 2323) women and 21.2% (n = 2266) men, mean age 49.86 ± 21.925 years (Me 56.00).Results. Only 17.1% (n = 1833) of the examined had an adequate level of 25(OH)D, 31.4% (n = 3362) people had an insufficient level of 25 (OH) D, 43.7% (n = 4681) of the examined – a deficiency of 25(OH)D and among 7.8% (n = 831) a severe deficiency of 25(OH)D was revealed. An adequate level of 25(OH)D was found only in the group of children under 3 years of age (mean value 40.55 ng/ml). From 4 to 7 years of age, there is a pronounced 2-fold decrease in the concentration of 25(OH)D in all seasons of the year. A consistently low content of 25(OH)D is observed in the period 8-10 years – 20.91 ng / ml, and in the period 11-18 years – 18.30 ng/ml. The lowest average values of 25(OH)D were found at the age of 11-18 years (18.30 ng/ml) and from 19 to 22 years (19.15 ng/ml), as well as in senile (76 years and above) age (19.05 ng/ml). The lowest median concentration of 25(OH)D is recorded in spring (17.70 ng/ml) and winter (18.80 ng/ml), with a slight positive trend in summer (20.40 ng/ml) and autumn (22.00 ng/ml) season.Conclusions. The optimal level of 25(OH)D was found in every fifth (17.1%) subject, vitamin D deficiency (20.01–30.00 ng/ml) was observed in every third (3362 people out of 10707 examined, 31.4%), a moderate calcidiol deficiency is recorded among 4681 people (43.7%), a severe deficiency of 25 (OH) D among the surveyed is 7.8%.
Results of migraine treatment depending on its menstrual association
Authors: Kiryanova E. A. , Kovalchuk N. A. , Tabeeva G. R. 
Published: September 2021

Introduction. Menstrual migraine attacks are considered more intense, prolonged, and resistant to treatment than non-menstrual ones. Currently, effect of preventive therapy on the course of menstrual-associated migraine has not been well studied; there are isolated studies on the beneficial effect of hormonal therapy on these attacks.The aim of the study was to compare the treatment results in the groups of menstrual-associated and non-menstrual migraine.Materials and methods. This comparative study included 91 patients aged 18 to 48 years (mean age 33,82 ± 8.4) with migraine and menstrual cycle. Depending on the menstrual association of migraine attacks, the patients were divided into 2 groups: group 1 – 54 patients (mean age 36.07 ± 7.37 years) with menstrual-associated migraine (MAM); group 2 – 37 patients (mean age 30.46 ± 8.81 years) with non-menstrual migraine (NMM).Results. Patients with MAM had more migraine days per month, more often received therapy associated with medication-overuse headache (MOH), more often had experience with triptans in comparison with NMM patients. After the treatment, the number of days with migraine per month in the MAM group remained more than in the NMM group: 8 or more days per month were noted in 36.4%, while among NMM patients – 10.8%. The transition to the new therapy with monoclonal antibodies to the CGRP receptor / ligand was positively assessed by 42.9% of MAM patients and 8.1% of NMM patients. Increased duration of hormonal therapy was characterized by a decrease in the number of days with migraines per month (cor = -0,28).Conclusion. In the MAM group, more severe diseases and frequent formation of MOH are observed, as well as a higher resistance to therapy than in NMM. In general, the use of standard therapy regimens for MAM is less effective than for NMM.
‘They will listen to women who speak but it ends there’: examining empowerment in the context of water and sanitation interventions in Ghana
Authors: Dery Florence , Bisung Elijah , Dickin Sarah , Atengdem Jeremiah 
Published: September 2021

Abstract
Gender plays a vital role in shaping access to and governance of water, sanitation, and hygiene (WASH) services in many contexts. Notably, women and men have different WASH-associated roles as well as varying access to resources and power. This study explores the meanings of women's empowerment in the WASH sector from the perspective of local stakeholders in the Asutifi North District, Ghana. A qualitative approach was employed, which involved 15 key informant interviews with community leaders, local government professionals, and WASH practitioners. Participants conceptualized empowerment in terms of four major themes: (1) availability of resources, (2) WASH information, (3) social and cultural structures, and (4) agency. Participants defined empowerment as a multifaceted process shaped by their physical, social, cultural, and political environments. The study provides researchers and practitioners with a greater understanding of the dimensions of empowerment that are relevant to strengthening WASH interventions and practices.
High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
Authors: Eftekhari-Kenzerki Raheleh , Solhjoo Kavous , Babaei Zahra , Rezanezhad Hassan , Abolghazi Ahmad , Taghipour Ali 
Published: September 2021

Abstract
Acanthamoeba spp. is a free-living amoeba that can cause major infections in humans, including keratitis and granulomatous encephalitis. Thus, water resources play an important role in transmitting Acanthamoeba spp. infection to humans. The purpose of this study was to investigate the presence of Acanthamoeba spp. in public swimming pools from three cities of Kerman Province, southeastern Iran. Eighty water samples of 20 public indoor swimming pools were taken from Kerman, Jiroft, and Kahnauj cities. Water temperature (°C), pH, and free chlorine concentration (ppm) were measured. Filtration and cultivation were applied on non-nutrient agar medium. The polymerase chain reaction was applied by using the genus-specific primers (JDP1 and JDP2) on positive samples; these primers can amplify the 423–551 bp fragment. Eighteen of the 20 swimming pools (including 32/80; 40% samples) were contaminated with Acanthamoeba spp. All swimming pools of Jiroft and Kahnauj and 88.2% of swimming pools in Kerman were contaminated. As such, all 32 Acanthamoeba isolates were amplified using the JDP primer pairs. Two genotypes, T3 and T4, were also identified. The present research is the first to report Acanthamoeba spp. in public swimming pools from Kerman Province. Due to high occurrence of this protozoan, it is recommended to use warning signs around swimming pools to create awareness of this infection.