bluuebunny/crossref_metadata_2025_split huggingface dataset:
IoT Big Data provenance scheme using blockchain on Hadoop ecosystem
Authors: Honar Pajooh Houshyar , Rashid Mohammed A. , Alam Fakhrul , Demidenko Serge 
Published: August 2021

AbstractThe diversity and sheer increase in the number of connected Internet of Things (IoT) devices have brought significant concerns associated with storing and protecting a large volume of IoT data. Storage volume requirements and computational costs are continuously rising in the conventional cloud-centric IoT structures. Besides, dependencies of the centralized server solution impose significant trust issues and make it vulnerable to security risks. In this paper, a layer-based distributed data storage design and implementation of a blockchain-enabled large-scale IoT system are proposed. It has been developed to mitigate the above-mentioned challenges by using the Hyperledger Fabric (HLF) platform for distributed ledger solutions. The need for a centralized server and a third-party auditor was eliminated by leveraging HLF peers performing transaction verifications and records audits in a big data system with the help of blockchain technology. The HLF blockchain facilitates storing the lightweight verification tags on the blockchain ledger. In contrast, the actual metadata are stored in the off-chain big data system to reduce the communication overheads and enhance data integrity. Additionally, a prototype has been implemented on embedded hardware showing the feasibility of deploying the proposed solution in IoT edge computing and big data ecosystems. Finally, experiments have been conducted to evaluate the performance of the proposed scheme in terms of its throughput, latency, communication, and computation costs. The obtained results have indicated the feasibility of the proposed solution to retrieve and store the provenance of large-scale IoT data within the Big Data ecosystem using the HLF blockchain. The experimental results show the throughput of about 600 transactions, 500 ms average response time, about 2–3% of the CPU consumption at the peer process and approximately 10–20% at the client node. The minimum latency remained below 1 s however, there is an increase in the maximum latency when the sending rate reached around 200 transactions per second (TPS).
Enhancement of co-production of lutein and protein in Chlorella sorokiniana FZU60 using different bioprocess operation strategies
Authors: Ma Ruijuan , Zhang Zhen , Tang Zhuzhen , Ho Shih-Hsin , Shi Xinguo , Liu Lemian , Xie Youping , Chen Jianfeng 
Published: August 2021

AbstractCo-production of multiple compounds is an efficient approach to enhance the economic feasibility of microalgae-based metabolites production. In this study, Chlorella sorokiniana FZU60 was cultivated under different bioprocess strategies to enhance the co-production of lutein and protein. Results showed that both lutein and protein content (7.72 and 538.06 mg/g, respectively) were highest at the onset of nitrogen deficiency under batch cultivation. Semi-batch III strategy, with 75% microalgal culture replacement by fresh medium, obtained similar content, productivity, and yield of lutein and protein as batch cultivation, demonstrating that it can be used for stable and continuous production. Fed-batch II strategy, feeding with 1/3 modified BG11 medium, achieved super-high lutein and protein yield (28.81 and 1592.77 mg/L, respectively), thus can be used for high-output production. Besides, two-stage strategy, combining light intensity shift and semi-batch cultivation, gained extremely high lutein and protein productivity (15.31 and 1080.41 mg/L/day, respectively), thereby is a good option for high-efficiency production. Moreover, the fed-batch II and two-stage strategy achieved high-quality lutein and protein, thus are promising for the co-production of lutein and protein in C. sorokiniana FZU60 for commercial application.
The effects of the molecular weights of hyaluronic acid on the immune responses
Authors: Lee Bo Mi , Park Sang Jun , Noh Insup , Kim Chun-Ho 
Published: August 2021

Abstract

Background
The molecular weight of hyaluronic acid (HyA) depends on the type of organ in the body. When HyA of the desired molecular weight is implanted into the human body for regeneration of damaged tissue, it is degraded by hyaluronidase in associated with an inflammatory response. This study sought to evaluate the effects of HyA molecular weight and concentration on pro- and anti-inflammatory responses in murine macrophages.

Methods

The structures and molecular weights of HyAs (LMW-10, MMW-100, MMW-500, and HMW-1,500) were confirmed by
1
H NMR and gel permeation chromatography (GPC), respectively. After treatment of murine macrophages with a low (10 µg/mL) or high (100 µg/mL) concentration of each molecular weight HyA, cells were stimulated with lipopolysaccharide (LPS) and changes in immune response in both LPS-stimulated and untreated macrophages were evaluated by assessing nitric oxide (NO) production, and analyzing expression of pro- and anti-inflammatory genes including by RT-PCR.

Results
Molecular weights of LMW-10, MMW-100, MMW-500, and HMW-1,500 were 13,241 ± 161, 96,531 ± 1,167, 512,657 ± 8,545, and 1,249,500 ± 37,477 Da, respectively. NO production by LPS-stimulated macrophages was decreased by increasing concentrations and molecular weights of HyA. At a high concentration of 100 µg/mL, HMW-1,500 reduced NO production in LPS-stimulated macrophages to about 45 %. Using NanoString technology, we also found that the immune-related genes TNF-α, IL-6, IL-1β, TGF-β1, IL-10, IL-11, CCL2, and Arg1 were specifically over-expressed in LPS-stimulated macrophages treated with various molecular weights of HyA. An RT-PCR analysis of gene expression showed that HMW-1,500 decreased expression of classically activated (M1) macrophage genes, such as TNF‐α, IL-6, CCL2, and IL-1β, in LPS-stimulated macrophages, whereas medium molecular-weight HyA (MMW-100 and MMW-500) instead increased expression levels of these genes. HMW-1,500 at a high concentration (100 µg/mL) significantly decreased expression of pro-inflammatory genes in LPS-stimulated macrophages. Expression of genes associated with anti-inflammatory responses (M2 phenotype), such as TGF-β1, IL-10, IL-11, and Arg1, were increased by high concentrations of MMW-500 and HMW-1,500 in LPS-stimulated macrophages.

Conclusions
High molecular-weight HyA (i.e., > 1,250 kDa) inhibits pro-inflammatory responses in LPS-stimulated macrophages and induces anti-inflammatory responses in a concentration dependent manner.
Tissue-specific systemic responses of the wild tobacco Nicotiana attenuata against stem-boring herbivore attack
Authors: Lee Gisuk , Joo Youngsung , Baldwin Ian T. , Kim Sang-Gyu 
Published: August 2021

Abstract
Background
Plants are able to optimize defense responses induced by various herbivores, which have different feeding strategies. Local and systemic responses within a plant after herbivory are essential to modulate herbivore-specific plant responses. For instance, leaf-chewing herbivores elicit jasmonic acid signaling, which result in the inductions of toxic chemicals in the attacked leaf (tissue-specific responses) and also in the other unattacked parts of the plant (systemic responses). Root herbivory induces toxic metabolites in the attacked root and alters the levels of transcripts and metabolites in the unattacked shoot. However, we have little knowledge of the local and systemic responses against stem-boring herbivores. In this study, we examined the systemic changes in metabolites in the wild tobacco Nicotiana attenuata, when the stem-boring herbivore Trichobaris mucorea attacks.

Results
To investigate the systemic responses of T. mucorea attacks, we measured the levels of jasmonic acid (JA), JA-dependent secondary metabolites, soluble sugars, and free amino acids in 7 distinct tissues of N. attenuata: leaf lamina with epidermis (LLE), leaf midrib (LM), stem epidermis (SE), stem pith (SP), stem vascular bundle (SV), root cortex with epidermis (RCE), and root vascular bundle (RV). The levels of JA were increased in all root tissues and in LM by T. mucorea attacks. The levels of chlorogenic acids (CGAs) and nicotine were increased in all stem tissues by T. mucorea. However, CGA was systematically induced in LM, and nicotine was systematically induced in LM and RCE. We further tested the resource allocation by measuring soluble sugars and free amino acids in plant tissues. T. mucorea attacks increased the level of free amino acids in all tissues except in LLE. The levels of soluble sugars were significantly decreased in SE and SP, but increased in RV.

Conclusions
The results reveal that plants have local- and systemic-specific responses in response to attack from a stem-boring herbivore. Interestingly, the level of induced secondary metabolites was not consistent with the systemic inductions of JA. Spatiotemporal resolution of plant defense responses against stem herbivory will be required to understand how a plant copes with attack from herbivores from different feeding guilds.
A volumetric magnetic resonance imaging study in migraine
Authors: Naguib Laila Elmously , Abdel Azim Ghada Saed , Abdellatif Mohammed Abdelrazek 
Published: August 2021

Abstract
Background
Although migraine phenotype has been widely described, the explanation of migraine pathophysiology still has a gap that might be partly bridged by neuroimaging investigations. The aim of the study is to assess volumetric brain changes in migraineurs compared with controls, and in episodic migraine in comparison to chronic type. Structural brain changes in migraineurs (with and without aura) were assessed by an automated segmentation method (Free Surfer). T1-weighted MRIs of 25 migraineurs (14 diagnosed as episodic type and 11 diagnosed as chronic migraine) and 25 headache-free controls were evaluated and processed.

Results
Migraine patients had significant reduction of the volume of total brain, grey matter, brain stem, cerebellum, basal ganglia, thalamus, hippocampus and amygdala in comparison to control subjects. Patients with chronic migraine had significant reduction in volume of total brain, grey matter, cerebellum and frontal lobe thickness in comparison to those with episodic migraine.

Conclusion
Migraineurs showed volumetric brain changes mainly in areas related to central processing of pain and in areas specific for migraine (such as brain stem) when compared to healthy controls. Chronic migraineurs showed significant reduction in grey matter, in areas involved in processing of pain, cognition and multisensory integration versus patients with episodic migraine, which adds insight into the pathophysiology of migraine as a progressive disorder that may have long-term impacts on the brain as regards structure and function.
Species and genetic variability of sweet potato viruses in China
Authors: Wang Yongjiang , Qin Yanhong , Wang Shuang , Zhang Desheng , Tian Yuting , Zhao Fumei , Wang Yingzhi , Lv Hui , Qiao Qi , Zhang Zhenchen 
Published: August 2021

AbstractChina is the world’s largest producer of sweet potato (Ipomoea batatas (L.) Lam.). Considering that there are numerous sweet potato-producing regions in China and sweet potato is a vegetatively propagated crop, the genetic diversity of sweet potato viruses could be high in the country. However, studies on species and genetic variabilities of sweet potato viruses in China are limited, making it difficult to prevent and control viral diseases in this crop. During 2014–2019, sweet potato samples with viral disease-like symptoms were randomly collected from sweet potato fields in 25 provinces in China. Twenty-one virus species, including 12 DNA and 9 RNA viruses, were identified in the samples using next-generation sequencing, polymerase chain reaction and rolling-circle amplification methods. One novel sweepovirus species, Sweet potato leaf curl Hubei virus (SPLCHbV), was identified. Two species, Sweet potato collusive virus and Tobacco mosaic virus, were identified for the first time in sweet potato in China. Full-length or nearly full-length genomic sequences of 111 isolates belonging to 18 viral species were obtained. Genome sequence comparisons of potyvirus isolates obtained in this study indicate that the genome of sweet potato virus 2 is highly conserved, whereas the other four potyviruses, sweet potato feathery mottle virus, sweet potato virus G, sweet potato latent virus and sweet potato virus C, exhibited a high genetic variability. The similarities among the 40 sweepovirus genomic sequences obtained from eight sweepovirus species are 67.0–99.8%. The eight sweepoviruses include 14 strains, of which 4 novel strains were identified from SPLCHbV and 1 from sweet potato leaf curl Guangxi virus. Five sweet potato chlorotic stunt virus (SPCSV) isolates obtained belong to the WA strain, and the genome sequences of SPCSV are highly conserved. Together, this study for the first time comprehensively reports the variability of sweet potato viruses in China.
First record of stable isotopes (δ13C, δ18O) and element ratios (Mg/Ca, Sr/Ca) of Middle to Late Jurassic belemnites from the Indian Himalayas and their potential for palaeoenvironmental reconstructions
Authors: Alberti Matthias , Fürsich Franz T. , Pandey Dhirendra K. , Andersen Nils , Garbe-Schönberg Dieter , Bhosale Suraj , Chaskar Ketan , Habermann Jörg M. 
Published: August 2021

AbstractMiddle to Late Jurassic belemnites from the Spiti and Zanskar valleys in the Indian Himalayas were used for stable isotope (δ13C, δ18O) and element (Mg/Ca, Sr/Ca) analyses. Although the Himalayan orogeny deformed and altered a large portion of the collected fossils, cathodoluminescence and scanning electron microscopy in combination with analyses of iron and manganese contents allowed the identification of belemnites believed to still retain their original chemical composition. Results indicate a long-term temperature decrease from the Middle Callovian–Oxfordian to the Tithonian, which is proposed to have been caused by a concomitant drift of eastern Gondwana into higher palaeolatitudes. Reconstructed absolute temperatures depend on the used equation and assumed δ18O value of seawater, but most likely varied between 17.6 °C to 27.6 °C in the Kimmeridgian and Tithonian with average values between 22 °C to 24 °C. This way, temperatures were similar to slightly warmer than today at comparable latitudes. The reconstruction of absolute temperatures for the Middle Callovian–Oxfordian was hindered by a larger number of poorly preserved belemnites representing this time interval.
Minimally invasive mitral valve repair for degenerative etiology: a comparative study
Authors: Fouly Mohamed Abdel Hafez , Mousa Tarek K. 
Published: August 2021

Abstract
Background
There is a paucity of data comparing the minimally invasive mitral valve repair (MiMVr) to the conventional approach in patients with degenerative disease. Our objective was to compare the outcomes of MiMVr to the traditional mitral valve repair through median sternotomy in patients with degenerative mitral valve disease. We conducted a retrospective study on 215 patients classified into two groups. Group 1 (n = 80) included those who had mitral valve repair through a right anterolateral video-assisted mini-thoracotomy, and group 2 (n = 135) was approached through a conventional median sternotomy. We compared the preoperative, operative, and postoperative data between groups. Both groups had echocardiographic follow-ups after 6 and 12 months.

Results
There was no difference in gender distribution between both groups, and patients who had median sternotomy were significantly older (median 37 (Q1-Q3, 29-44) vs. 54 (48-60) years; P < 0.001). Cardiopulmonary bypass (134.5 (130-138.5) vs. 99 (97-104) min; P < 0.001) and ischemic times (99 (95-105.5) vs. 78 (75-81) min; P < 0.001) were significantly shorter in patients who had median sternotomy. Patients with MiMVr had significantly lower blood loss (370 (315-390) vs. 550 (490-600) ml; P < 0.001) and ICU stay (5 (4.5-6) vs. 7 (7-8) days; P < 0.001). There was no difference between both groups regarding re-exploration for bleeding, postoperative stroke, wound infection, renal failure, and mortality. As regards postoperative echocardiography follow-up at 6 and 12 months after the operation, there were no significant changes in the mean mitral valve gradient within each group; however, the mean gradient was lower in the MiMVr group (3 (3-3.5) vs. 4 (3-5) mmHg; P < 0.001). There was no significant difference between both groups regarding mitral regurgitation severity during 6 and 12 months follow-up.

Conclusion
Minimally invasive mitral valve repair in patients with degenerative pathology could be an alternative to conventional mitral valve surgery with comparable short-term and long-term outcomes.
Stakeholder perspectives to inform adaptation of a hypertension treatment program in primary healthcare centers in the Federal Capital Territory, Nigeria: a qualitative study
Authors: Okoli Rosemary C. B. , Shedul Gabriel , Hirschhorn Lisa R. , Orji Ikechukwu A. , Ojo Tunde M. , Egenti Nonye , Omitiran Kasarachi , Akor Blessing , Baldridge Abigail S. , Huffman Mark D. , Ojji Dike , Kandula Namratha R. 
Published: August 2021

Abstract
Background
Implementing an evidence-based hypertension program in primary healthcare centers (PHCs) in the Federal Capital Territory, Nigeria is an opportunity to improve hypertension diagnosis, treatment, and control and reduce deaths from cardiovascular diseases. This qualitative research study was conducted in Nigerian PHCs with patients, non-physician health workers, administrators and primary care physicians to inform contextual adaptations of Kaiser Permanente Northern California's hypertension model and the World Health Organization’s HEARTS technical package for the system-level, Hypertension Treatment in Nigeria (HTN) Program.

Methods
Purposive sampling in 8 PHCs identified patients (n = 8), non-physician health workers (n = 12), administrators (n = 3), and primary care physicians (n = 6) for focus group discussions and interviews. The Primary Health Care Performance Initiative (PHCPI) conceptual framework and Consolidated Framework for Implementation Research (CFIR) domains were used to develop semi-structured interviews (Appendix 1, Supplemental Materials) and coding guides. Content analysis identified multilevel factors that would influence program implementation.

Results
Participants perceived the need to strengthen four major health system inputs across CFIR domains for successful adaptation of the HTN Program components: (1) reliable drug supply and blood pressure measurement equipment, (2) enable and empower community healthcare workers to participate in team-based care through training and education, (3) information systems to track patients and medication supply chain, and (4) a primary healthcare system that could offer a broader package of health services to meet patient needs. Specific features of the PHCPI framework considered important included: accessible and person-centered care, provider availability and competence, coordination of care, and proactive community outreach. Participants also identified patient-level factors, such as knowledge and beliefs about hypertension, and financial and transportation barriers that could be addressed with better communication, home visits, and drug financing. Participants recommended using existing community structures, such as village health committees and popular opinion leaders, to improve knowledge and demand for the HTN Program.

Conclusions
These results provide information on specific primary care and community contextual factors that can support or hinder implementation and sustainability of an evidence-based, system-level hypertension program in the Federal Capital Territory, Nigeria, with the ultimate aim of scaling it to other parts of the country.
Addressing the importance of microplastic particles as vectors for long-range transport of chemical contaminants: perspective in relation to prioritizing research and regulatory actions
Authors: Gouin Todd 
Published: August 2021

AbstractOver the last several years there has been increasing concern regarding the environmental fate and potential global transport of plastic debris, particularly in the form of microplastic particles (MPs). The global transport of MPs has also triggered concerns regarding the potential role that its mobility may represent towards influencing the long-range environmental transport (LRET) of particle-bound chemicals, particularly the large number of chemicals known to be added to plastic. This perspective considers the various lines-of-evidence that might be used towards understanding the LRET of persistent organic pollutants (POPs). For instance, it has been proposed that the LRET of POPs is facilitated by global fractionation processes that facilitate the mobility of chemicals from source regions towards remote locations, such as the polar regions, where they have the potential to accumulate. These processes are influenced by the physicochemical properties of POPs and can result in various transport mechanisms influencing environmental fate and transport. Here I suggest that there are similarities that can be drawn, whereby knowledge of how differences in the physicochemical properties of MPs relative to different emission scenarios, can influence the relative importance of sequestration processes that may result in global fractionation of MPs. Several challenges are identified throughout the perspective, with an urgent need towards the development and application of standard sampling and analytical methods being identified as critical for enabling datasets to be reliably compared for use in better understanding potential source-receptor relationships, as well as advancing the characterization and quantification of various environmental fate processes. In many instances, it is suggested that advances in our understanding can be facilitated based on knowledge obtained in other areas of research, such as in relation to studies developing tools to evaluate the mobility of particulate organic matter in aqueous environments or from studies investigating the fate and mobility of atmospheric particulates. Recognizing that not all MPs are equal, with respect to environmental fate and toxicological effects, knowledge regarding which types of MPs are likely to be subject to LRET can only strengthen our ability to evaluate their role as vectors of transport for plastic associated chemicals and the associated risks that their LRET may represent. Nevertheless, several outstanding issues remain that would benefit from constructive discussions between all stakeholders. It is anticipated that this perspective can play a role in initiating those discussions.
Cryptanalysis of quantum secure direct communication protocol with mutual authentication based on single photons and Bell states
Authors: Das Nayana , Paul Goutam 
Published: August 2021

Abstract
Recently, Yan et al. proposed a quantum secure direct communication (QSDC) protocol with authentication using single photons and Einstein-Podolsky-Rosen (EPR) pairs (Yan L. et al., Comput. Mater. Contin., 63 (2020) 1297). In this work, we show that the above QSDC protocol is secure neither against intercept-and-resend attack, nor against impersonation attack. With any of these two types of attacks, an eavesdropper can recover the full secret message. We also propose a suitable modification of this protocol, which not only defeats the above attacks, but also resists all other common attacks. Thus, our modified protocol provides an improvement over the existing one in terms of security.