bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment Of Ecotoxicity Of Thallium By Biological Indicator Of Soil Condition
Authors: Kolesnikov Sergey , Kazeev Kamil , Akimenko Yuliya , Ter-Misakyants Tigran , Nevedomaya Elena 
Published: February 2021

Abstract
Thallium is a rare and highly toxic heavy metal. The scale and degree of soil contamination with thallium are increasing every year, and the associated environmental risks have been insufficiently investigated. Model laboratory experiments on the ordinary chernozem pollution with thallium oxide (III) have been carried out. It has been found that the ordinary chernozem pollution with thallium worsened its biological activity. The total bacterial count, the abundance of the Azotobacter bacteria, the activity of catalase, the activity of dehydrogenases, and the germination of radish seeds decreased. The degree of deterioration of the chernozem biological activity depends on the thallium concentrations in soil and the period from the moment of contamination. In the vast majority of cases there is a direct correlation between the thallium content in the soil and the level of soil biological activity reduction. The greatest negative impact of thallium pollution on chernozem biological activity has manifested itself 30 days after contamination. On the 90th day, there was a pronounced tendency to restore the chernozem biological activity. However, the chernozem biological activity did not restore to the initial uncontaminated soil values. At contamination of chernozem with thallium, microbiological indices (the bacterial count and the abundance of the Azotobacter bacteria) were more sensitive, and the enzyme activity indicators (catalase, dehydrogenase) and phytotoxicity (germination of plant seeds) were more informative. All used indicators of biological activity demonstrated high sensitivity to soil contamination with thallium and high correlation coefficients with the thallium content in the soil. Therefore, these biological indicators should be used for monitoring, diagnosis, indication and regulation of soil contamination with thallium.
Discovery and Validation of Novel Protein Markers in Mucosa of Portal Hypertensive Gastropathy
Authors: Zhu Ying , Xu Wen , Hu Wei , Wang Fang , Zhou Yan , Xu Jianguo , Gong Wei 
Published: February 2021

Abstract
Background: Portal hypertension induced esophageal and gastric varix bleeding is the main cause of death among patients of decompensated liver cirrhosis. Therefore, a standardized, biomarker-based test, to make an early-stage non-invasive risk assessment of portal hypertension, is highly desirable. However, no fit-for-purpose biomarkers have yet been identified. Methods: We conducted a pilot study consisting of 5 portal hypertensive gastropathy (PHG) patients and 5 normal controls, sampling the gastric mucosa of normal controls and PHG patients before and after endoscopic cyanoacrylate injection, using label-free quantitative (LFQ) mass spectrometry, to identify potential biomarker candidates in gastric mucosa from PHG patients and normal controls. Then we further used parallel reaction monitoring (PRM) to verify the abundance of the targeted protein. Results: LFQ analyses identified 423 significantly differentially expressed proteins. 17 protein that significantly elevated in the gastric mucosa of PHG patients were further validated using PRM. Conclusions: This is the first application of an LFQ-PRM workflow to identify and validate PHG–specific biomarkers in patient gastric mucosa samples. Our findings lay the foundation for comprehending the molecular mechanisms of PHG pathogenesis, and provide potential applications for useful biomarkers in early diagnosis and treatment.Trial Registration: Trial registration was completed (ChiCTR2000029840) on February 25, 2020.
Distribution of Human Papillomavirus in Precancerous and Cancerous Cervical Neoplasia in Tunisian Women
Authors: Rhouma Rahima Bel Haj , Ardhaoui Monia , Fehri Emna , Marzougui Asma , Assili Thalja , Guizani Ikram , Boubaker Med Samir , Ennaifer Emna 
Published: February 2021

Abstract
BackgroundHigh-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC), that is a major health problem worldwide and the second most prevalent gynecological cancer among Tunisian women. Preventive tools against CC are based on screening and prophylactic vaccines. Improving the preventive strategies as well as the therapeutic algorithms needs the understanding of HPV distribution in cervical lesions in Tunisian women. MethodsA total of 200 formalin-fixed paraffine embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. ResultsThe mean age of participants was 38.97 years and 54.5% were over 40 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed in women over 30 years old. All samples were positive for Beta Globin PCR. Global HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% in CINI, 82% in CINII/CINIII and 85% in CC. HR-HPV was statically associated with cervical intraepithelial neoplasia (p<10-3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (33,33%) was the most detected type, followed by HPV18 (28,6%), HPV 45 (25%) and HPV 16 (21,7%). HPV 45(62%), HPV 18 (47%) were the most detected in CC. HPV58, 59, 66 were only detected in CC and associated with HPV45,18 and HPV16. HPV39, 31, 33, 52, 56, 68 and HPV70 was associated only with CINI. ConclusionsThese findings show that HR-HPV represents 54,6 % of all infections in all cervical lesions. Five HR-HPV (35, 18, 45,16, 51) were detected in CINII/CINIII with a high incidence of HPV 35, 18, 45, and 16 that are included in the proposed vaccines. These findings give useful information for vaccine implementation consideration as well as personalized decision algorithms of intra-epithelial cervical lesions in Tunisian women.
WITHDRAWN: Model of Dependence Between the Parameters of Surface Roughness and the Parameters of Form and Location During the Machining by Drills with the use of Artificial Neural Networks
Authors: Krivokapić Zdravko , Vučurević Radoslav , Dasic Predrag V. , Ivanković Petar 
Published: February 2021

Abstract
This paper presents a model of dependence between the parameters of surface roughness and the parameters of cylindricity and eccentricity in drilling operation for the enhancing steel EN 42CrMo4, hardness 28 HRC, with twist drills DIN 338 made of high-speed steel EN HS6-5-2, with normal blade. The quality of machining, besides the accuracy of measures, completely determined with the values of the parameters of the surface roughness and the parameters of form and location, hence this paper is oriented to the creating models between parameters of the quality of a machined surface and parameters of deviations from form and position. By the developing models based on artificial neural networks and using experimental results, it is possible to analyse the quality of machining on the basis of parameters of a surface roughness.
Predictors of Congenital Anomalies Among Newborns in Arsi Zone Public Hospitals, Southeast Ethiopia: a Case-control Study
Authors: Jemal Sudi , Fentahun Engidaw , Oumer Mohammed , Muche Abebe 
Published: February 2021

Abstract
Background: Congenital anomaly is a partial or complete structural and/or functional defect during intrauterine life. Globally, major congenital anomalies account for 6% of all newborns among which about 94% of cases occurred in developing countries. In spite of its public health importance, very limited studies are reported in Ethiopia, and hardly any study in Arsi Zone.Objectives: To determine the predictors of congenital anomalies among newborns in Arsi Zone Public Hospitals, Southeast Ethiopia.Methods: A multi-center institutional-based case-control study was conducted in 418 (105 cases and 313 controls) of newborns in Arsi Zone Public Hospitals. Descriptive analysis, binary and multivariable logistic regressions were implemented.Results: In this study, women who have been drinking alcohol during pregnancy were 3.48 times more prone to have newborns with congenital anomalies than their counterparts (AOR=3.48; 95% CI: 1.38, 8.74). The likelihood of having a newborn with congenital anomalies was six and four times higher for women who had a maternal illness (AOR=6.10; 95%CI: 2.39, 15.57) and chewing khat during pregnancy (AOR=4; 95%CI: 1.49, 10.65), respectively. Moreover, the lack of folic acid supplementation and pesticides during pregnancy were 3.25 and 4.76 times more likely to experience a newborn with congenital anomalies, respectively. Conclusion: Alcohol drinking, maternal illness, khat chewing, and chemical exposure during pregnancy had a significant association with the occurrence of congenital anomalies. While, taking folic acid supplements had a protective effect. Health experts and the community should take these factors into consideration and act accordingly.
Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae&nbsp;before Infection
Authors: Zhang Zhuoqun , Zhao Yifan , An Tai , Yu Han , Bi Xiangqi , Liu Haixu , Xu Ying , Yang Zhiyue , Chen Yufei , Wen Jingzhi 
Published: February 2021

Abstract
Purpose: Phytophthora sojae does not infect the nonhost maize (Zea mays L.) but could infect the nonhost common bean (Phaseolus vulgaris L.). Soybean seed exudates participate in mediating host resistance to P. sojae before infection. This study aimed to elucidate the role of nonhost seed exudates in mediating nonhost resistance to P. sojae before infection. Methods: The response behavior of P. sojae zoospores to the seed exudates was determined using an assay chamber and a concave slide, and the proteomes of P. sojae zoospores treated with the seed exudates were analysed with the tandem mass tag (TMT) method. The key proteins were quantified by parallel reaction monitoring (PRM).Results: Maize seed exudates exerted a repellent effect on zoospores, whereas common bean seed exudates did not exhibit any attraction to zoospores but could dissolve the cysts. The key proteins related to zoospores chemotaxis showed no significant changes in response to maize seed exudates, but the key proteins in arachidonic acid pathway were downregulated and controlled the repellent behavior of zoospores. Proteins protecting the cell membrane structure were significantly downregulated in zoospores responding to common bean seed exudates, which confirmed the bacteriolytic effect of common bean seed exudates on cysts. Conclusion: Maize and common bean seed exudates mediate part of the nonhost resistance via different mechanisms prior to P. sojae infection. The immune of maize to P. sojae is due to the repellent effect of maize seed exudates on zoospores. Common bean seed exudates participate in mediating nonhost resistance by dissolving cysts.
The Difference of Acetabular Cup Orientation Between Two Surgical Sides After Primary Total Hip Arthroplasty by Right-handed Surgeons
Authors: Xiong Ao , liu su , Li Guoqing , Weng Jian , Wang Deli , Zeng Hui 
Published: February 2021

Abstract
Background: We performed the retrospective cohort study to compare the acetabular cup orientation, including anteversion angle (AA) and inclination angle (IA), of dominant hand side and non-dominant hand side after primary total hip arthroplasty (THA) by right-handed orthopedic surgeons. Methods: Between January 2018 and December 2018, 290 patients who aged below 60 years and underwent primary THA were retrospective screened. Patients who had hemiarthroplasty, previous hip surgery, ankylosing spondylitis, developmental dysplasia of hip (DDH, Crowe type-Ⅲ and type-Ⅳ), severe comorbidity, missing information, inferior quality radiographs were excluded. According to the surgery side, all patients were divided into left group and right group. Postoperative plain radiographs were analyzed to compare the AA and IA between left and right side. Univariate and stepwise multivariable linear regression to control included confounding factors. Stratified analysis was performed to identify whether the operation approach can affect the result, including anterolateral (ALA) and posterolateral approach (PLA). Results: The mean AA was 17.7° (range 6.0° to 30.0°) and 21.0° (range 9.5° to 35.0°) for the left and right side respectively. The mean difference was 3.28° (95% CI: 1.92 – 4.64; P<0.001). The mean IA was 41.1° (range 24.0° to 59.0°) and 40.1° (range 20.5° to 56.0°) for the left and right side respectively (P=0.314). 113 patients' AA within the “safe zone” in the left (93.4 %), while the right was 93 patients (82.3 %) (P=0.009). 95 patients' IA within the “safe zone” in the left (78.5 %), while the right was 97 patients (85.8 %) (P=0.144). The IA of ALA group was smaller than PLA group in both sides. The mean difference was 3.98° (95% CI: 1.22 - 6.74; P=0.005). Conclusions: We concluded that AA in left side may be more accurate than right side after primary THA by right-handed surgeons. The IA was no difference between the two sides, while it was smaller in ALA than in PLA. The results are still needed to be verified in future.
PUMILIO Proteins in Colorectal Cancer: Tumor Growth Promoting Function and Potential as Therapeutic Targets
Authors: Gong Yuanyuan , Liu Zukai , Yuan Yihang , Yang Zhenzhen , Zhang Jiawei , Lu Qin , Wang Wei , Fang Chao , Lin Haifan , Liu Sanhong 
Published: February 2021

Abstract
PUMILIO (PUM) proteins belong to the highly conserved PUF family post-transcriptional regulators involved in diverse biological processes. However, their function in carcinogenesis remains under explored. Here, we found that the expression of Pum1 and Pum2 are increased in clinical colorectal cancer (CRC). Intestine-specific knockout of Pum1 and Pum2 significantly inhibited the progression of colitis associated cancer in the AOM/DSS model. Knockout or knockdown of Pum1 and/or Pum2 resulted in a significant decrease in the tumorigenicity. In addition, delayed G1/S transition was observed. We identified p21/Cdkn1a as direct target of PUM1, and abrogation of the PUM1 binding site in p21 resulted in decreased tumor cell growth as well as delayed G1/S transition. Furthermore, intravenous injection of nanoparticle-encapsulated anti-Pum1 and Pum2 siRNAs reduced colorectal tumor growth in murine orthotopic colon cancer models. These findings reveal a tumor growth promoting role of PUM proteins in CRC and its potential as therapeutic targets.
A Ferroptosis-Related Gene Signature for Lung Function and Quality of Life in Patients with Idiopathic Pulmonary Fibrosis
Authors: Li Yupeng , Ning Shangwei , Yang Yi , Chen Hong , Wang Chen 
Published: February 2021

Abstract
Background: Rapid advances in genetic and genomic technologies have begun to reshape our understanding of idiopathic pulmonary fibrosis (IPF). Ferroptosis, an iron-dependent form of regulated cell death, play an important role in the development of IPF. Therefore, our study aimed to explore the role of ferroptosis-related genes (FRGs) and their correlation with lung dysfunction and quality of life in patients with IPF. Methods: Datasets were acquired by researching the Gene Expression Omnibus. FRGs were acquired by researching GeneCard database and PubMed. Ferroptosis-related differentially expressed genes (FRDEGs) were identified according to integrating FRGs and the DEGs identified in the GSE110147 dataset. Candidate key genes were identified from the miRNA-target FRDEGs network and protein-protein interactions (PPI) network. The relationship between key genes and lung function or quality of life was calculated using the GSE32537 datasets.Results: 293 FRGs were obtained, and 71 FRDEGs were identified. According to enrichment analysis, cell growth and death and pathways associated cancer were the important pathways, and significant biological processes were mainly consisted of cellular responses to stimulus and various situations. In addition, this study constructed an PPI network and a miRNA-target network based on the 71 FRDEGs, determined 19 candidate key genes. Furthermore, acyl-CoA synthetase long chain family member 1 (ACSL1), integrin subunit beta 8 (ITGB8) and ceruloplasmin (CP) were identified as the key genes. The expression level of ACSL1 was the strongest predictor for lung function (negatively) including percent predicted forced vital capacity (FVC% predicted) and percent predicted diffusion capacity of the lung for carbon monoxide (Dlco% predicted) and quality of life (negatively). In addition, ITGB8 and CP were negatively associated with FVC% predicted. According to DrugBank and PubMed, 4 drugs and 16 drugs have been found to act on ACSL1 and CP, respectively. Conclusion: These results imply that FRGs may shed new understanding on disease mechanism and provide potential biomarkers and therapy target to predict IPF progression.
WITHDRAWN: N6-Methyladenosine Modification of PTTG3P Contributes to Colorectal Cancer Progression via IGF2BP2
Authors: zheng yang , yu guilin , xie longfei , wang yue , zhao guohua 
Published: February 2021

Abstract
Background N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) emerged as crucial players in colorectal cancer (CRC) progression, but the m6A modified lncRNA PTTG3P in CRC are still need to be systematically defined. Methods qRT-PCR was adopted to measure the PTTG3P expression. Survival analysis was used to explore the correlation between the expression of PTTG3P and CRC patients prognosis. Receiver operating curve (ROC) was tested to evaluate the PTTG3P predictive ability. Functional studies were examined by CCK-8, glucose uptake, lactate assay, ATP assay, ECAR assay and xenograft mice model. Mechanistic studies were explored by GSEA, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) and RNA immunoprecipitation (RIP).Results PTTG3P was upregulated in CRC and closely related to poor prognosis. Through gain and loss of function approaches, PTTG3P facilitated proliferation and glycolysis through Hippo pathway, and glycolysis inhibitor (2-DG ,3-BG) and LDHA knockdown could rescue cell proliferation. Mechanically, m6A methylation induced the elevation of PTTG3P by increasing its stability , and insulin like growth factor-2 mRNA binding proteins 2 (IGF2BP2) involved in the progression. Finally, rescue assays validated the effect of METTL3/PTTG3P/YAP1 axis in CRC progression. Conclusions m6A-induced PTTG3P could facilitates CRC development via interacting with IGF2BP2, which provides a predictive biomarker and theraperutic target for CRC.
CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway
Authors: Wang Le , Ren Bingcheng , Zhong Yue , NAN Yang 
Published: February 2021

Abstract
Background Glioma is the most common primary intracranial tumor. Abnormal expression of CBX2(ChromoBox2) is associated with tumorigenesis and tumor development. Methods TCGA data in UALCAN showed that CBX2 was overexpressed in glioma tissue. To confirm the role of CBX2 in glioma, we regulated the level of CBX2 and conducted colony formation, Transwell, and CCK-8 assays to verify the effect of CBX2. Results The results showed that CBX2 knockdown reduced glioma cell proliferation and invasion and that the cells were less tumorigenic. CBX2 overexpression induced glioma cell proliferation and invasion and glioma stem cell self-renewal. The animal experiments showed that CBX2 knockdown inhibited glioma growth and improved survival time. CBX2 knockdown inhibited activation of the Akt/PI3K pathway. EGF rescued the effects of CBX2. Conclusion We hypothesized that CBX2 induced the growth and invasion of glioma cells via the Akt/PI3K pathway.
Design, implementation and Evaluation of Self-Care Program in the Prevention of Breast Cancer Among Women in Isfahan: A Community-Based Participatory Action Research Protocol
Authors: kianpour Maryam , Taleghani Fariba , Noroozi Mahnaz , Savabi-Esfahani Mitra , boroumandfar Zahra , Hashemi Maryam , Changiz Tahereh , Javanmard Shaghayegh Haghjooy 
Published: February 2021

Abstract
Background: Breast cancer is one of the most prevalent cancers among women in Isfahan, Iran but unfortunately its prevention is not desirable in this city. This disease poses several healths, social and economic burdens upon women. In order to promote women's self-care in breast cancer prevention, this study aims to design, implement and evaluate a self-care program in the prevention of breast cancer among women in Isfahan through using a community-based participatory action research method.Methods: The present study will be conducted based on a community-based participatory action research in Isfahan, Iran. The participatory action research in this study will include four general phases of organizing, action planning, action and rethinking. The mentioned phases will be as follows: In the organizing phase, the needs of the participants and the action research settings are examined. This means that the current situation is identified and the views of the process owners are evaluated. In the action planning phase, using the results of the first phase, some strategies are designed in order to promote self-care behaviors in the prevention of breast cancer among women in Isfahan. In the implementation phase, the selected strategies are implemented with the help of the process owners. Finally, in the rethinking phase, the results of the implementation of the strategies are monitored and evaluated, and this cycle continues until the intended results are achieved. Discussion: Changing the role of individuals from a passive state in the care process to an aware and active state requires motivation, responsibility and active participation of people in the disease control process. Moreover, there are many cultural and social factors which affect the active participation of Iranian women. As such, using a community-based participatory action research, people can be involved in promoting their health.
Enhancing the Performance and Recyclability of Polyaniline/TiO2 Hybrid Nanocomposite by Immobilizing with Zein/Hydroxyethyl cellulose Composites for Removal of Anionic dyes
Authors: Gelaw Tadesse Bassie , Sarojini Balladka Kunhanna 
Published: February 2021

Abstract
Fabricating a stable, recyclable, and eco-friendly photocatalyst for dye treatment is vital in sustaining a clean ecosystem. In this regard, polyaniline/TiO2 (PANI/TiO2) photocatalyst was immobilized by zein/hydroxyethyl cellulose (zein/HEC) adhesive to enhance recyclability and catalytic activity. The blending of zein/HEC/PANI/TiO2 photocatalyst involves insitu oxidative polymerization, followed by immobilization with zein/HEC functionalized composites. The PANI/TiO2 composite was successfully grafted with the adhesive through physicochemical interaction, as evidenced by field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), and Powder X-ray diffractometer (XRD). The simultaneous thermal analysis (STA) results show that the photocatalyst has the best thermal stability relative to PANI and PANI/TiO2 in the recommended range of dye degradation temperature. The effect of external factors like TiO2 nanoparticle proportion, pH of the solution, and catalyst dosage was studied in response to dye degradation capacity. The synthesized catalyst is efficient to degrade methyl orange in a wide range of pH. The kinetics of the catalysis reaction obeys first order kinetics. The maximum degradation efficiency achieved was 97.9 and 84.3% in the presence and absence of light, respectively. The catalyst was easily recovered by decantation, and its catalytic efficacy was more than 94% after five cycles. Hence, it is a promising alternative for decolorizing anionic dyes from wastewater.