bluuebunny/crossref_metadata_2025_split huggingface dataset:
The lncRNA TEX41 is upregulated in pediatric B-Cells Acute Lymphoblastic Leukemia and it is necessary for leukemic cell growth
Authors: Orlandella Francesca Maria , Smaldone Giovanni , Salvatore Giuliana , Vitagliano Luigi , Cianflone Alessandra , Parasole Rosanna , Beneduce Giuliana , Menna Giuseppe , Salvatore Marco , Mirabelli Peppino 
Published: July 2021

Abstract
Background
Long non-coding RNAs (lncRNAs) represent a diverse class of RNAs involved in the regulation of various physiological and pathological cellular processes, including transcription, intracellular trafficking, and chromosome remodeling. LncRNAs deregulation was linked to the development and progression of various cancer types, such as acute leukemias. In this context, lncRNAs were also evaluated as a novel class of biomarkers for cancer diagnosis and prognosis. Here, we analyzed TEX41 in childhood B cell acute lymphoid leukemia (B-ALL).

Methods
Total RNA was extracted from pediatric B-ALL patients (at diagnosis and after induction of therapy) and from healthy subjects. Total RNA was also extracted from different leukemia cell line models. The expression level of TEX41 was evaluated by q-RT-PCR. Also, the dataset deposited by St. Jude Children’s Research Hospital was consulted. Furthermore, the silencing of TEX41 in RS4;11 cell line was obtained by 2′-Deoxy, 2′Fluroarabino Nucleic Acids (2′F-ANAs) Oligonucleotides, and the effect on cell proliferation was evaluated. Cell cycle progression and its regulators were analyzed by flow cytometry and immunoblotting.

Results
We exploited the St Jude Cloud database and found that TEX41 is a lncRNA primarily expressed in the case of B-ALL (n = 79) while its expression levels are low/absent for T-cell ALL (n = 25) and acute myeloid leukemia (n = 38). The association of TEX41 with B-ALL was confirmed by real-time PCR assays. TEX41 disclosed increased expression levels in bone marrow from patients with B-ALL at diagnosis, while its expression levels became low or absent when retested in Bone Marrow cells of the same patient after 1 month of induction therapy. Also, silencing experiments performed on RS4;11 cells showed that TEX41 downregulation impaired in vitro leukemic cell growth determining their arrest in the G2-M phase and the deregulation of cell cycle proteins.

Conclusions
Our findings highlight that TEX41 is an upregulated lncRNA in the case of B-ALL and this feature makes it a novel potential biomarker for the diagnosis of this leukemia subtype in pediatric patients. Finally, TEX41 expression seems to be critical for leukemic proliferation, indeed, silencing experiments targeting TEX41 mRNA in the RS4;11 cell line hampered in vitro cell growth and cell cycle progression, by inducing G2-M arrest as confirmed propidium iodide staining and by the upregulation of p53 and p21 proteins.
Integration of tumor inflammation, cell proliferation, and traditional biomarkers improves prediction of immunotherapy resistance and response
Authors: Pabla Sarabjot , Seager R. J. , Van Roey Erik , Gao Shuang , Hoefer Carrie , Nesline Mary K. , DePietro Paul , Burgher Blake , Andreas Jonathan , Giamo Vincent , Wang Yirong , Lenzo Felicia L. , Schoenborn Margot , Zhang Shengle , Klein Roger , Glenn Sean T. , Conroy Jeffrey M. 
Published: July 2021

Abstract
Background
Contemporary to the rapidly evolving landscape of cancer immunotherapy is the equally changing understanding of immune tumor microenvironments (TMEs) which is crucial to the success of these therapies. Their reliance on a robust host immune response necessitates clinical grade measurements of immune TMEs at diagnosis. In this study, we describe a stable tumor immunogenic profile describing immune TMEs in multiple tumor types with ability to predict clinical benefit from immune checkpoint inhibitors (ICIs).

Methods
A tumor immunogenic signature (TIGS) was derived from targeted RNA-sequencing (RNA-seq) and gene expression analysis of 1323 clinical solid tumor cases spanning 35 histologies using unsupervised analysis. TIGS correlation with ICI response and survival was assessed in a retrospective cohort of NSCLC, melanoma and RCC tumor blocks, alone and combined with TMB, PD-L1 IHC and cell proliferation biomarkers.

Results
Unsupervised clustering of RNA-seq profiles uncovered a 161 gene signature where T cell and B cell activation, IFNg, chemokine, cytokine and interleukin pathways are over-represented. Mean expression of these genes produced three distinct TIGS score categories: strong (n = 384/1323; 29.02%), moderate (n = 354/1323; 26.76%), and weak (n = 585/1323; 44.22%). Strong TIGS tumors presented an improved ICI response rate of 37% (30/81); with highest response rate advantage occurring in NSCLC (ORR = 36.6%; 16/44; p = 0.051). Similarly, overall survival for strong TIGS tumors trended upward (median = 25 months; p = 0.19). Integrating the TIGS score categories with neoplastic influence quantified via cell proliferation showed highly proliferative and strong TIGS tumors correlate with significantly higher ICI ORR than poorly proliferative and weak TIGS tumors [14.28%; p = 0.0006]. Importantly, we noted that strong TIGS and highly [median = not achieved; p = 0.025] or moderately [median = 16.2 months; p = 0.025] proliferative tumors had significantly better survival compared to weak TIGS, highly proliferative tumors [median = 7.03 months]. Importantly, TIGS discriminates subpopulations of potential ICI responders that were considered negative for response by TMB and PD-L1.

Conclusions
TIGS is a comprehensive and informative measurement of immune TME that effectively characterizes host immune response to ICIs in multiple tumors. The results indicate that when combined with PD-L1, TMB and cell proliferation, TIGS provides greater context of both immune and neoplastic influences on the TME for implementation into clinical practice.
Forest canopy scattering properties with signal of opportunity reflectometry: theoretical simulations
Authors: Wu Xuerui , Calabia Andrés , Xu Jin , Bai Weihua , Guo Peng 
Published: July 2021

AbstractIn recent years, signal of opportunity reflectometry (SoOp-R) has become a promising remote sensing technique. This emerging technique employs the reflected signals from existing Global Navigation Satellite System (GNSS) or communication satellites to estimate geophysical parameters for Earth observation, such as wind speed, altimetry, significant wave height, soil moisture, etc. While its application for forest canopy monitoring is still in the initial stage, there are still many unknown relations between vegetation parameters and actual observations, and a proper theoretical basis needs to be established for simulation and analysis of the different observation geometries. In this paper, we develop a bistatic scattering model with various polarizations at different frequency bands. Our improved model is based on the first-order radiative transfer equation, and is developed based on the wave synthesis technique, after which it can be used for circular polarization signals in bistatic radar systems, i.e. the typical configuration of SoOp-R. We analyze the simulations of the P (0.25–0.5 GHz), L (0.5–1.5 GHz), C (4–8 GHz), and X (8–12 GHz) bands at the backscattering, specular cone, bistatic scattering, and perpendicular planes. The contributions of the different components to the total scattering are also analyzed. The results show that the coherent scattering at the specular cone is larger than the non-coherent scattering, while trunk-dominated forest canopy has strong scattering at the aforementioned different directions. Variations of canopy parameters such as trunk and branch diameters, tree density, and vegetation water content are also simulated at the specular cone plane, showing strong dependence on the final bistatic scattering observation. The simulation results show that the SoOp-R technique has a great potential for monitoring of canopy parameters.
Zonal aspects of the influence of forest cover change on runoff in northern river basins of Central Siberia
Authors: Onuchin A. , Burenina Т. , Shvidenko А. , Prysov D. , Musokhranova A. 
Published: July 2021

Abstract
Background
Assessment of the reasons for the ambiguous influence of forests on the structure of the water balance is the subject of heated debate among forest hydrologists. Influencing the components of total evaporation, forest vegetation makes a significant contribution to the process of runoff formation, but this process has specific features in different geographical zones. The issues of the influence of forest vegetation on river runoff in the zonal aspect have not been sufficiently studied.

Results
Based on the analysis of the dependence of river runoff on forest cover, using the example of nine catchments located in the forest-tundra, northern and middle taiga of Northern Eurasia, it is shown that the share of forest cover in the total catchment area (percentage of forest cover, FCP) has different effects on runoff formation. Numerical experiments with the developed empirical models have shown that an increase in forest cover in the catchment area in northern latitudes contributes to an increase in runoff, while in the southern direction (in the middle taiga) extensive woody cover of catchments “works” to reduce runoff. The effectiveness of geographical zonality in regards to the influence of forests on runoff is more pronounced in the forest-tundra zone than in the zones of northern and middle taiga.

Conclusion
The study of this problem allowed us to analyze various aspects of the hydrological role of forests, and to show that forest ecosystems, depending on environmental conditions and the spatial distribution of forest cover, can transform water regimes in different ways. Despite the fact that the process of river runoff formation is controlled by many factors, such as temperature conditions, precipitation regime, geomorphology and the presence of permafrost, the models obtained allow us to reveal general trends in the dependence of the annual river runoff on the percentage of forest cover, at the level of catchments. The results obtained are consistent with the concept of geographic determinism, which explains the contradictions that exist in assessing the hydrological role of forests in various geographical and climatic conditions. The results of the study may serve as the basis for regulation of the forest cover of northern Eurasian river basins in order to obtain the desired hydrological effect depending on environmental and economic conditions.
Effectiveness of a continuous interactive communication system for home care nursing assistance of peritoneal dialysis
Authors: Terada Kohsuke , Yanagida Yorito , Yan Tomohiro , Funakoshi Toshiki , Hirama Akio , Kashiwagi Tetsuya , Sakai Yukinao 
Published: July 2021

Abstract
Background
The Japanese population is aging quickly. New methods of supporting peritoneal dialysis (PD) for elderly patients are essential if we are to increase the number of such patients.

Methods
We established a two-way communication system between a central hospital and stations for visiting nurses. Home care nurses provided physicians at the central hospital with clinical findings for patients undergoing assisted PD. We compared 11 patients undergoing PD assisted by home care nurses in continuous interactive communication with the central hospital (cases) with 11 patients undergoing unassisted PD who were matched by sex, primary disease, estimated glomerular filtration rate at the start of PD, and age at the start of PD (controls). The variables compared were hospitalization due to heart failure, exit-site infection, peritonitis, and the composite of these causes during a 1-year period. In addition, we compared patient clinical factors between groups.

Results
Although differences in the number of hospitalizations attributable to exit-site infection, peritonitis, and heart failure were not significant, the difference in the number of hospitalizations attributable to a composite of these causes was significant. Exit-site scores were significantly better in the case group than in the control group.

Conclusions
PD assisted by home care nurses using an established continuous interactive communication system was associated with significantly better exit-site scores. Fewer cases than controls were hospitalized.
Researchers’ perspectives on methodological challenges and outcomes selection in interventional studies targeting medication adherence in rheumatic diseases: an OMERACT-adherence study
Authors: Salmasi Shahrzad , Kelly Ayano , Bartlett Susan J. , de Wit Maarten , March Lyn , Tong Allison , Tugwell Peter , Tymms Kathleen , Verstappen Suzanne , De Vera Mary A. ,   On behalf of the OMERACT-Adherence working group
Published: July 2021

Abstract
Background
Research on adherence interventions in rheumatology is limited by methodological issues, particularly heterogeneous outcomes. We aimed to describe researchers’ experiences with conducting interventional studies targeting medication adherence in rheumatology and their perspectives on establishing core outcomes.

Methods
Semi-structured interviews using audio conference were conducted with researchers who had conducted an adherence study of any design in the past 10 years. Data collection and thematic analysis were performed iteratively, until saturation.

Results
We interviewed 13 researchers, most of whom worked in academia and specialized in epidemiology and/or health services research. We identified three themes: 1) improving measurement of adherence (considering all phases of adherence, using appropriate and relevant measures, and establishing clinically meaningful thresholds); 2) challenges in designing and appraising adherence intervention studies (considering the confusion over a plethora of outcomes, difficulties with powering studies to demonstrate meaningful changes, and suboptimal descriptions of adherence interventions in published studies); and 3) advancing outcome assessment in adherence intervention studies (capturing rationale for developing a core domain set as well as recommendations and anticipated challenges by participants).

Conclusions
Uniquely gathering perspectives from international adherence researchers, our findings led to researcher-informed recommendations for improving adherence research including specifying the targeted adherence phase in designing interventions and studies and providing a glossary of terms to promote consistency in reporting. We also identified recommendations for developing a core domain set for interventional studies targeting medication adherence including involvement of patients, clinicians, and other stakeholders and methodological and practical considerations to establish rigor and support uptake.
The diagnostic yield of whole exome sequencing as a first approach in consanguineous Omani renal ciliopathy syndrome patients
Authors: Al Alawi Intisar , Al Riyami Mohammed , Barroso-Gil Miguel , Powell Laura , Olinger Eric , Al Salmi Issa , Sayer John A. 
Published: July 2021

Background: Whole exome sequencing (WES) is becoming part of routine clinical and diagnostic practice. In the investigation of inherited cystic kidney disease and renal ciliopathy syndromes, WES has been extensively applied in research studies as well as for diagnostic utility to detect various novel genes and variants. The yield of WES critically depends on the characteristics of the patient population. Methods: In this study, we selected 8 unrelated Omani children, presenting with renal ciliopathy syndromes with a positive family history and originating from consanguineous families. We performed WES in affected children to determine the genetic cause of disease and to test the yield of this approach, coupled with homozygosity mapping, in this highly selected population. DNA library construction and WES was carried out using SureSelect Human All Exon V6 Enrichment Kit and Illumina HiSeq platform. For variants filtering and annotation Qiagen Variant Ingenuity tool was used. Nexus copy number software from BioDiscovery was used for evaluation of copy number variants and whole gene deletions. Patient and parental DNA was used to confirm mutations and the segregation of alleles using Sanger sequencing. Results: Genetic analysis identified 4 potential causative homozygous variants each confirmed by Sanger sequencing in 4 clinically relevant ciliopathy syndrome genes, (TMEM231, TMEM138, WDR19 and BBS9), leading to an overall diagnostic yield of 50%. Conclusions: WES coupled with homozygosity mapping provided a diagnostic yield of 50% in this selected population. This genetic approach needs to be embedded into clinical practise to allow confirmation of clinical diagnosis, to inform genetic screening as well as family planning decisions. Half of the patients remain without diagnosis highlighting the technical and interpretational hurdles that need to be overcome in the future.
CovidLife: a resource to understand mental health, well-being and behaviour during the COVID-19 pandemic in the UK
Authors: Fawns-Ritchie Chloe , Altschul Drew M. , Campbell Archie , Huggins Charlotte , Nangle Clifford , Dawson Rebecca , Edwards Rachel , Flaig Robin , Hartley Louise , Levein Christie , McCartney Daniel L. , Bell David , Douglas Elaine , Deary Ian J. , Hayward Caroline , Marioni Riccardo E. , McIntosh Andrew M. , Sudlow Cathie , Porteous David J. 
Published: July 2021

CovidLife is a longitudinal observational study designed to investigate the impact of the COVID-19 pandemic on mental health, well-being and behaviour in adults living in the UK. In total, 18,518 participants (mean age = 56.43, SD = 14.35) completed the first CovidLife questionnaire (CovidLife1) between April and June 2020. To date, participants have completed two follow-up assessments. CovidLife2 took place between July and August 2020 (n = 11,319), and CovidLife3 took place in February 2021 (n = 10,386). A range of social and psychological measures were administered at each wave including assessments of anxiety, depression, well-being, loneliness and isolation. Information on sociodemographic, health, and economic circumstances was also collected. Questions also assessed information on COVID-19 infections and symptoms, compliance to COVID-19 restrictions, and opinions on the UK and Scottish Governments’ handling of the pandemic. CovidLife includes a subsample of 4,847 participants from the Generation Scotland cohort (N~24,000, collected 2006-2011); a well-characterised cohort of families in Scotland with pre-pandemic data on mental health, physical health, lifestyle, and socioeconomic factors, along with biochemical and genomic data derived from biological samples. These participants also consented to their study data being linked to Scottish health records. CovidLife and Generation Scotland data can be accessed and used by external researchers following approval from the Generation Scotland Access Committee. CovidLife can be used to investigate mental health, well-being and behaviour during COVID-19; how these vary according to sociodemographic, health and economic circumstances; and how these change over time. The Generation Scotland subsample with pre-pandemic data and linkage to health records can be used to investigate the predictors of health and well-being during COVID-19 and the future health consequences of the COVID-19 pandemic.
Pregnancy related cultural food practices among Pakistani women in the UK: a qualitative study
Authors: Hussain Basharat , Bardi Josephine NwaAmaka , Fatima Tehniyat 
Published: July 2021

Background Food practices are influenced by cultural traditions which continue to be important among immigrant groups in their new homeland, especially during significant life events, such as marriage, pregnancy and funerals, as well as for religious ceremonies and festivities. Aim This study aims to explore pregnancy related food practices among first generation Pakistani women living in the UK. Methods A total of 10 first-generation immigrant women were recruited through a voluntary organisation following a convenience sampling technique and were interviewed. Findings The data revealed that the women attached different symbolic meanings to their food practices, particularly relating to pregnancy. These findings were based on 1) their understanding of the changes their body experienced during pregnancy and 2) responding to these changes through the consumption and/or avoidance of certain foods. Conclusion Understanding the cultural contexts of Pakistani women in the UK is important in order to promote healthy food patterns for that cohort during pregnancy.
Intervention for preventing postpartum depression: a systematic review of studies in Indonesia
Authors: Widiasih Restuning , Rais Yulia , Fithriyah Noor , Damayanti Damayanti , Puspitasari Neneng Ratnanengsih 
Published: July 2021

Background Many researches in Indonesia have analysed various interventions to prevent postpartum depression (PPD) by health professionals. Aim This literature review aims to identify various health interventions to prevent PPD in Indonesia. Methods Searching articles used several electronic databases, namely Google Scholar and Indonesian database. The articles included in this review are full-text articles, published from 2008–2017. Results Three types of actions have been identified to prevent PPD, namely counselling, music therapy and aromatherapy. The research instrument such as Edinburgh Postnatal Depression Scale and Zung Self-Rate Anxiety Scale were effective to measure the risk of PPD. Conclusion Midwives can apply methods according to the patient's needs in order to prevent PPD.
Improving outcomes for older people with diabetes
Authors: Morris David 
Published: July 2021

Older people with diabetes have unique challenges. David Morris discusses the importance of individualising care for this group of people An individualised approach aiming to maximise safety, preserve autonomy and improve quality of life is needed when helping an older person to manage their diabetes. It is important to interpret the older person's diabetes in the context of their overall health concerns, including reference to co-morbidities, cognitive function, lifestyle, social setting, and life expectancy, and practice nurses are well placed to work in partnership with people with diabetes to achieve this. Pharmacological treatment goals must be realistic, acknowledging the metabolic consequences of old age, the risks of hypoglycaemia and the dangers of polypharmacy.
Hacia una nueva racionalidad política a partir de las relaciones sociedad-Estado
Authors: Balladares Burgos Jorge Antonio 
Published: July 2021

Hoy en día se percibe una crisis en torno a las relaciones de la sociedad con el estado producto del descrédito por no sentirse identificados con el gobierno de turno, los actos de corrupción, la ineficacia de las acciones en torno a desastres naturales y humanos como la pandemia del Covid-19. Esta realidad planetaria nos invita a pensar en una nueva racionalidad política que sea incluyente reconociendo las diferencias y diversidades del ciudadano común. Para este cometido, el presente artículo realiza una revisión de la literatura desde un recorrido histórico desde diferentes autores de la filosofía política sobre los sentidos y las tensiones en las relaciones entre la sociedad y el estado. Luego se plantea lo que es una nueva racionalidad política a partir de un sujeto político emergente que, ante las adversidades, las situaciones de marginalidad y pobreza, hace prevalecer el sentido de una conciencia colectiva. Desde un enfoque hermenéutico, se realiza una definición de lo político, como una forma alternativa de la praxis política que busca recuperar el sentido de organización, convivencia y toma de decisiones desde nuestra condición como seres políticos. De esta manera, las relaciones sociedad-estado serán resituadas para la búsqueda de un nuevo orden post-covid más justo, solidario, tolerante y resiliente.