bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility Study and Direct Extraction of Endogenous Free Metallic Cations Combining Hemodialysis and Chelating Polymer
Authors: Natuzzi Marco , Grange Coralie , Gréa Thomas , Brichart Thomas , Aigle Axel , Bechet Denise , Hautefeuille Benoit , Thomas Eloise , Ayoub Jean-Yves , Bonnet Jeanne-Marie , Louzier Vanessa , Allaouchiche Bernard , Couturier Aymeric , Montembault Alexandra , Oliveira Paula Nunes , David Laurent , Lux François , Tillement Olivier 
Published: July 2021

Abstract
In this article, we report the conception and the use of dialysis based medical device for extraction of metals. The medical device is obtained by addition in the dialysate of a functionnalized chitosan that can chelate endogenous metals like iron or copper. The water soluble functionnalized chitosan is obtained after controlled reacetylation and addition of DOTAGA. Due to the high mass of chitosan, the polymer cannot cross through the membrane and the metals are trapped in the dialysate during hemodialysis. Copper captation has been evaluated in vitro using an hemodialysis protocol. Feasibility study has been performed on healthy sheep showing no acute toxicity of the whole procedure and first insights of metallic extraction even on healthy animals.
Arsenic Induced Free Radicals Mediated Genotoxicity and Protective Features of Ocimum Gratissium L.
Authors: Mishra Nikhil , Tripathi Seema , KUMAR ANIL 
Published: July 2021

Abstract
Arsenic toxicity is a worldwide geogenic problem for large number of populations. The major problem of arsenicosis is generation of free radicals and related genotoxicity, In the present study three plants species Ocimum gratissimum L., Ocimum sanctum, Ocimum basilicum, were examined by both in vivo and in vitro methods in Oryctolagus cuniculus L to know the free radical scavenging property in Sodium Arsenite induced oxidative stress and related genotoxicity. Oxidative stress was evaluated considering MDA, GSH, SOD, CAT & GPx, and genotoxicity was examined by comet assay and RAPD test. In the present study, Na3AsO3 was found to generate free radicals in lymphocytes of O. cuniculus and best recovery was reported (MDA-4.78 ± 0.05; GSH-2.87 ± 0.50;SOD-1.78 ± 0.03;CAT-2.72 ± 0.02;GPx-7.43 ± 0.01) under exposure of ethanolic leaf extract of O. gratissimum. Na3AsO3 was also found to cause polymorphic bands and increased comet tail in lymphocytes which was found significantly recovered by exposure of ethanolic leaf extract of O. gratissimum.
Multiple Outbreaks Resulting from Asymptomatic Infection in Spreading of COVID-19
Authors: Li Jun , Ma Mingju 
Published: July 2021

Abstract
In this paper, we establish a compartmental model for the transmission of COVID-19 with both symptomatic and asymptomatic infections. The difference is that in our model, we consider the infection caused by multiple contacts with asymptomatic infected population. Through the dynamic analysis of the model, we found that when there is only one contact with an asymptomatic infected people, the system has a unique threshold to control the disease. When there is two contact with asymptomatic infected population, the system will undergo forward bifurcation, backward bifurcation, saddle-node bifurcation, supercritical Hopf bifurcation, subcritical Hopf bifurcation, and Bogdanov-Takens bifurcation with codimension 2. Finally, we give The complete bifurcation diagram and global phase diagram of the system, and the biological significance of our results are also given.
Characteristics and Prognostic Factors of Patients With Chondrosarcoma Older Than 60 Years ：an Analysis of 610 Cases from the SEER Database
Authors: Chen Tailong , Zhou Nan , Tang Zhongxin , Guo Xing , Wang Wengang 
Published: July 2021

Abstract
Background

Chondrosarcoma is a rare type of bone tumor which more commonly found in adults range from 40 to 60 years old. Few studies has described the characteristic and prognostic factors of patients older than 60 years. This study aimed to study this feature and identify the prognostic factors based on SEER database.
Methods

Thus, we collected clinicopathological data of chondrosarcoma patients in the Surveillance, Epidemiology, and End Results registry database from 1975 to 2018, and then use the Kaplan-Meier to analyze the patients’ survival. We also utilize Cox proportional hazard model to explore the prognostic factors and relevant characteristic including patients’ baseline demographics (age, race, and gender), tumor characteristics (tumor extension, histologic subtype, therapy, primary site, stage and grade.
Results

After the implementation of exclusion criteria, there were 610 patients with chondrosarcoma older than 60 years. Our data showed that the incidence of chondrosarcoma is slightly higher in men than in women (52.3% vs 47.7%). In general, 90.8% of tumor had metastasized to distant sites. Meanwhile, 41.8% of tumors occurred in axial location (pelvis, spine, and ribs), 50.8% of tumors occurred in extremity (long or short bones of the upper or lower extremity), and 7.4% in other location (mandible, skull, and other atypical locations). Dedifferentiated chondrosarcoma (hazard ratio [HR] = 2.553; 95% confidence interval [CI] = 1.754–3.716), grade (g2:HR:=1.299; 95% CI:=0.888-1.900, g3:HR = 1.839;95% CI = 1.174–2.881, g4:HR = 3.284,95%CI = 2.053–5.253), distant metastasis (HR = 3.264; 95% CI= (2.288–4.058), non-surgery perform (HR = 2.854; 95% CI = 2.022–4.028) were independent risk factors for 5-year overall survival.
Conclusion

In conclusion, higher grade, non-surgery perform, dedifferentiated chondrosarcoma and distant metastasis indicated worse prognosis survival. Surgery can significantly improve the survival time of patients.
Trends in Veteran Population Use to Veterans Affairs Chiropractic Care
Authors: Burdick Ryan , Corcoran Kelsey , Zhao Xiwen , Lisi Anthony 
Published: July 2021

Abstract
BackgroundThe US Department of Veterans Affairs (VA) has initiated various approaches to provide chiropractic care to Veterans. Prior work has shown substantial increase in use of VA chiropractic care between fiscal years (FY) 2005-2016. However, the extent of the availability of these services to the Veteran population remains unclear. The purpose of this study was to analyze the rate of Veteran use of VA chiropractic services, both from on-site care at VA facilities and VA purchased care from community care providers. This study analyzed facility characteristics associated with chiropractic use by both care delivery mechanisms (on-site and in the community).MethodsCross-sectional analyses of administrative data were conducted for FY 2014-19. Data were obtained from VA’s Corporate Data Warehouse. The variables extracted included number of unique Veterans receiving VA chiropractic care on-site and in the community, total Veteran population of the VA facilities, size of the VA chiropractic workforce (measured as Full-Time Equivalent, FTE), and facility characteristics (geographic region and the facility complexity). Descriptive statistics, mixed model, and multivariant models were used to analyze data.ResultsUse of VA chiropractic care increased over the six-year period for both on-site and community care. National average for on-site use of the population was 1.27% in FY14 and 1.48% in FY19. Community care use was 0.29% and 1.76% for the same years. Use at individual facilities varied widely in each FY. Factors such as chiropractor FTE, geographic locations, and the complexity of the VA facility are associated with use of chiropractic services.ConclusionThe VA has expanded the non-pharmacologic treatments available to Veterans by providing chiropractic services, yet chiropractic use remains low compared to other US populations. As Veterans have a high prevalence of pain and musculoskeletal conditions, continued work to assess and achieve the optimal levels of chiropractic use in this population is warranted.
Preoperative Fasting Blood Glucose Levels and the Risk of Contrast-induced Nephropathy in Patients With Diabetes and Pre-diabetes Receiving Coronary Arteriography or Percutaneous Coronary Intervention: a Cross-sectional Study
Authors: Zhang Peng , Fu Han , Yang Shicheng , Guo Zhigang , Fu Naikuan 
Published: July 2021

Abstract
Background: Diabetes mellitus is an independent risk factor for contrast-induced nephropathy in patients undergoing coronary arteriography /percutaneous coronary intervention. In this study, we evaluated whether preoperative fasting blood glucose levels in diabetic and prediabetic patients who received CAG/PCI impacted the occurrence of postoperative CIN.Methods: We reviewed the incidence of preoperative FBG and postoperative CIN in 3437 patients with CAG/PCI from January 1, 2019, to May 28, 2021 in Tianjin Chest Hospital. Patients were divided into 5 groups based on their preoperative FBG levels. Blood samples were collected at admission and at 48 h and 72 h after operation to determine the serum creatinine levels of patients. P for trend was used to analyze the trend between preoperative FBG levels and the increased risk of CIN. Univariable and multivariable logistic regression analysis were used to exclude the influence of the confounding factors, and some high-risk confounders were selected for subgroup analysis.Results: We found that patients with elevated preoperative FBG levels had higher body mass index (BMI), received PCI more often, and had higher basal SCr levels. The incidence of CIN in the 5 groups of patients was 6.7%, 10.5%, 17.5%, 18.4%, and 23.2%. The trend test was used to determine the odds ratio (OR) (1.000, 1.425, 2.489, 2.704, 3.234). Multivariate logistic regression analysis confirmed that in the main high-risk subgroups including BMI, PCI, hypertension, and age>65, patients with elevated preoperative FBG levels (≥150 mg/dL) had a higher risk of CIN. There was a significant relationship between higher preoperative FBG levels and greater risk for CIN in patients with HbA1c 6.5%-8.0% (P=0.007) and HbA1c 8.0%-9.5% (P=0.011). Conclusions: Results of our cross-sectional study shows that elevated preoperative FBG levels are independently associated with the risk of CIN in diabetic and prediabetic patients undergoing CAG/PCI, and that the incidence of CIN gradually increases with an increase in preoperative FBG levels. Therefore, patients with elevated preoperative FBG at admission should be carefully monitored and more active measures should be taken to prevent CIN.
Development and validation of a prognostic model to predict clinical deterioration in hospitalized patients with COVID-19
Authors: Azadbakht Javid , Rashedi Sina , Kooraki Soheil , Kowsari Hamed , Tabibian Elnaz 
Published: July 2021

Abstract
Objectives We aimed to develop and validate a prognostic model to predict clinical deterioration defined as either death or intensive care unit admission of hospitalized COVID-19 patients.Methods This prospective, multicenter study investigated 172 consecutive hospitalized COVID-19 patients who underwent a chest computed tomography (CT) scan between March 20 and April 30, 2020 (development cohort), as well as an independent sample of 40 consecutive patients for external validation (validation cohort). The clinical, laboratory, and radiologic data were gathered, and logistic regression along with receiver operating characteristic (ROC) curve analysis was performed.Results The overall clinical deterioration rates of the development and validation cohorts were 28.4% (49 of 172) and 30% (12 of 40), respectively. Seven predictors were included in the scoring system with a total score of 15: CT severity score\(\ge\)15 (Odds Ratio (OR)=6.34, 4 points), pleural effusion (OR = 6.80, 2 points), symptom onset to admission ≤ 6 days (OR = 2.44, 2 points), age\(\ge\)70 years (OR = 2.44, 2 points), diabetes mellitus (OR = 2.24, 2 points), dyspnea (OR = 2.17, 1.5 points), and abnormal leukocyte count (OR = 1.89, 1.5 points). The area under the ROC curve for the scoring system in the development and validation cohorts was 0.823 (CI [0.751–0.895]) and 0.558 (CI [0.340–0.775]), respectively.Conclusion This study provided a new easy-to-calculate scoring system with external validation for hospitalized COVID-19 patients to predict clinical deterioration based on a combination of seven clinical, laboratory, and radiologic parameters.
Low-temperature Plasma Jet Activated Media Induces Apoptosis in Bladder Cancer Cells via the Cytochrome c/caspase 3 Pathway
Authors: Fukuhara Hideo , Szili Endre J. , Oh Jun-Seok , Chiaki Kawada , Yamamoto Shinkuro , Kurabayashi Atsushi , Furihata Mutsuo , Tsuda Masayuki , Furuta Hiroshi , Lindsay Howard D. , Short Robert D. , Hatta Akimitsu , Inoue Keiji 
Published: July 2021

Abstract
Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pre-treatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma activated media (PAM) in bladder cancer cells. PAM treatment increases up regulation of p21 and down regulation of Cyclin D and CdK4 leading to cell cycle arrest, and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production induce apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo via the pro-apoptotic caspase 3/cytochrome c pathway. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.
Correlations obtained from optical spectra of Fe-pnictides using an extended Drude-Lorentz model
Authors: Lee Seokbae , Seo Yu-Seong , Roh Seulki , Song Dongjoon , Eisaki Hirosh , Hwang Jungseek 
Published: July 2021

Abstract
We introduce an analysis model, an extended Drude–Lorentz model, and apply it to Fe-pnictide systems to extract their electron–boson spectral density functions (or correlation spectra). The extended Drude–Lorentz model consists of an extended Drude mode for describing correlated charge carriers and Lorentz modes for interband transitions. The extended Drude mode can be obtained by a reverse process starting from the electron–boson spectral density function and extending to the optical self-energy, and eventually, to the optical conductivity. Using the extended Drude–Lorentz model, we obtained the electron–boson spectral density functions of K-doped BaFe 2 As 2 (Ba-122) at four different doping levels. We discuss the doping-dependent properties of the electron–boson spectral density function of K-doped Ba-122. We also can include pseudogap effects in the model using this new approach. Therefore, this new approach is very helpful for understanding and analyzing measured optical spectra of strongly correlation electron systems, including high-temperature superconductors (cuprates and Fe-pnictides).
EGF signaling promotes the lineage conversion of astrocytes into oligodendroglias
Authors: Liu Xinyu , Xie Lesi , Li Jiao , Li Conghui , Zheng Kang , Zhao Xiaofeng , Yang Aifen , Xu Xiaofeng , Tao Huaping , Qiu Mengsheng , Yang Junlin 
Published: July 2021

Abstract
Background

The conversion of astrocytes activated by nerve injuries to oligodendrocytes is not only beneficial to axonal remyelination, but also helpful for reversal of glial scar. Recent studies have shown that Sox10 transcription factor can achieve this transdifferentiation process in collaboration with some unknown factors in the pathological microenvironment. The extracellular factors underlying the cell fate switching are not known.
Methods

Astrocytes were obtained from mouse cortical dissociation culture and purified by differential adherent properties. The lineage conversion of astrocytes into oligodendrocyte lineage cells was carried out by Sox10-expressing virus infection both in vitro and in vivo, meanwhile, epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) inhibitor Gefitinib were adopted to investigate the function of EGF signaling in this fate transition process. Pharmacological inhibition analyses were performed to examine the pathway connecting the EGF with the expression of oligodendrogenic genes and cell fate transdifferentiation.
Results

EGF treatment facilitated the Sox10-induced transformation of astrocytes to O4+ induced oligodendrocyte precursor cells (iOPCs) in vitro. The transdifferentiation of astrocytes to iOPCs went through two distinct but interconnected processes: (1) dedifferentiation of astrocytes to astrocyte precursor cells (APCs); (2) transformation of APCs to iOPCs, EGF signaling was involved in both processes. And EGF triggered astrocytes to express oligodendrogenic genes Olig1 and Olig2 by activating extracellular signal-regulated kinase 1 and 2 (Erk1/2) pathway. In addition, we discovered that EGF can enhance astrocyte transdifferentiation in injured spinal cord tissues.
Conclusions

These findings provide strong evidence that EGF facilitates the transdifferentiation of astrocytes to oligodendroglias, and suggest that targeting the EGF-EGFR-Erk1/2 signaling axis may represent a novel therapeutic strategy for myelin repair in injured central nervous system (CNS) tissues.
Transcriptome analysis revealed the effect of selenium yeast on improving eggshell quality in aged laying hens
Authors: Liu Zhexi , Cao Yutao , Ai Yue , Yin Xiaonan , Wang Lingli , Wang Mengyao , Zhang Bingkun , Wu Keliang , Guo Yuming , han hongbing 
Published: July 2021

Abstract
Background

Egg internal and eggshell quality were deteriorated in aging laying hens. Improving egg and shell quality can prolong the laying cycle. Although, selenium yeast (SY) has been found with the potential to enhance the laying performance and egg quality, the underlying mechanisms have not been investigated. Therefore, we investigated the effect and molecule mechanism of selenium yeast on egg and shell quality in aged laying hens.
Methods

Three hundred 76-week-old Jing Hong laying hens were divided into four equal treatments and fed with Se-deficient diet (SD), 0.15, 0.30, 0.45 mg/kg selenium yeast diet (SY0.15, SY0.30 and SY0.45). At the end of the trial, we evaluated the plasma and tissue selenium content, plasma antioxidant capacity and egg quality. Transcriptomic analysis was performed to investigate the boosting effect of selenium yeast on eggshell quality. The weighted gene co-expression network analysis (WGCNA) analysis was performed to screen out the key candidate genes related with eggshell quality affected by selenium yeast.
Results

Dietary selenium yeast supplementation enhanced the eggshell strength (SY0.45) (P < 0.01) and reduced the shell translucence (SY0.15, SY0.30 and SY0.45) to ameliorate the eggshell quality. Moreover, body Se status and plasma antioxidant capacity were significantly enhanced by selenium yeast supplementation (P < 0.05), and showed a dose-dependent effect. Transcriptomic and WGCNA analysis identified some key candidate genes involved in eggshell quality including cell migration inducing hyaluronidase 1 (CEMIP), ovalbumin (OVAL), solute carrier family 6 member 17 (SLC6A17), solute carrier family 13 member 5 (SLC13A5), proopiomelanocortin (POMC) and proenkephalin (PENK). Functional enrichment analysis revealed that selenium may alter the biological process including eggshell mineralization, ion transport and laying period to affect eggshell formation.
Conclusion

Selenium yeast enhanced eggshell strength and reduced the shell translucence by improving antioxidant capacity, selenium status, and regulating the processes of shell mineralization, ion transport and laying period. These findings provide a novel molecule biomarker for affecting eggshell quality regulated by selenium.
Impact of white-mater mask selection on DTI histogram-based metrics as potential biomarkers in cerebral small vessel disease
Authors: Fouto Ana R , Nunes Rita G. , Pinto Joana , Alves Luísa , Calado Sofia , Gonçalves Carina , Rebolo Margarida , Viana-Baptista Miguel , Vilela Pedro , Figueiredo Patrícia 
Published: July 2021

Abstract
Purpose Histogram-based metrics extracted from diffusion-tensor imaging (DTI) have been suggested as potential biomarkers for cerebral small vessel disease (SVD), but methods and results have varied across studies. This work aims to assess the impact of mask selection for extracting histogram-based metrics of fractional anisotropy (FA) and mean diffusivity (MD) on their sensitivity as SVD biomarkers.Methods DTI data were collected from 17 SVD patients and 12 healthy controls. For each participant, FA and MD maps were estimated; from these, histograms were computed on two alternative whole-brain white-matter masks: normal-appearing white-matter (NAWM) and mean FA tract skeleton (TBSS). Histogram-based metrics (median, peak height, peak width, peak value) were extracted from the FA and MD maps. These were compared between patients and controls, and correlated with the patients’ cognitive scores (executive function and processing speed).Results White matter mask selection significantly impacted FA and MD histogram metrics and affected their ability to discriminate between groups. Moreover, we observed that the mask can influence the correlations with cognitive measures. Nevertheless, the MD peak height and MD peak width metrics remained significantly correlated with executive function, regardless of the mask.Conclusion Our results corroborate previous reports and further support the value of DTI histogram-based metrics as SVD biomarkers. However, they also highlight the importance of the processing methodology, in particular the choice of white matter mask, as hence the urgent need to mitigate the lack of standardized MRI data-processing pipelines.
Research on the Mechanisms of Internal Gear Honing by use of Cone-shape Honing Wheel with Tool Tilt Angle
Authors: Guo Zheng , Li Yan , Guo Wen-Chao , Zhang Wu-Gang , Gao Feng , Zhang Dong-Ya 
Published: July 2021

Abstract
Ring gear is an important transmission component, but restricted by its ring-shape structure, the tooth surfaces are pretty hard to be finished after heat treatment. Honing is the most commonly used finishing technique for external gear, but for ring gear, it is inconvenient. In this paper, a new type of cone-shape honing wheel is proposed and the honing mechanism is studied for improving the honing performance of ring gear. First of all, the mathematical model of the honing technology by use of cone-shape honing wheel is built, and the merits of the new honing method are discussed. Then, the contact conditions between the honing wheel and the work gear are analyzed, and it is found that the tool tilt angle of the honing wheel has good influences on the honing mark distribution of the work gear. Finally, the honing simulations for ring gear by use of cone-shape honing wheel are carried out, by which the feasibility of the new honing technique are verified.