bluuebunny/crossref_metadata_2025_split huggingface dataset:
Late Quaternary Tectonics along the Peri-Adriatic Sector of the Apenninic Chain (Central-Southern Italy): Inspecting Active Shortening through Topographic Relief and Fluvial Network Analyses
Authors: Ferrarini Federica , Arrowsmith J. Ramón , Brozzetti Francesco , de Nardis Rita , Cirillo Daniele , Whipple Kelin X. , Lavecchia Giusy 
Published: November 2021

Abstract
Active compressional tectonics along the outer front of the Apenninic-Maghrebian chain (Italy) is well documented along the northern and central segments and in Sicily. On the other hand, the Southern Apenninic Outer Front (SAOF) orogenic activity is well established only until the Lower-Middle Pleistocene. We address the hypothesis of its subsequent late Quaternary activity in central-southern Italy (Abruzzo and Molise regions). We integrated topographic and fluvial network analyses along with morphotectonic investigation of fluvial terraces to identify evidence of differential rock uplift. We compared the results with the main geolithological units, known structural elements, and long-term deformation history from seismic line interpretation. We found variable evidence suggesting localized rock uplift in the Abruzzo region along the SAOF (Abruzzo Citeriore Basal Thrust segment) and inward structures on its hanging wall (Casoli-Bomba high), as well as along part of the Struttura Costiera thrust. Middle-to-Late Pleistocene deformation is constrained by terrace tilting and disruption along the Pescara river. Localized shortening along segments of the Apenninic Outer Front could explain the observed pattern of anomalies which is difficult to explain with long-wavelength regional uplift alone. Our reconstruction is consistent with the long-term deformation of the area and agrees with its seismotectonic setting. Despite the low deformation rate context and the peculiar geological setting which challenges the interpretation of the topographic and geomorphic signals, this study compels reconsideration, in terms of seismic hazard assessment, of the existence of late Quaternary active thrusting in central-southern Italy.
The predictive value of red blood cell distribution width and platelet-to -lymphocyte ratio for acute kidney injury in critically ill patients
Authors: Tang Yan , Jiang Fen , Zhang Li , Xiang Jiaxuan , Lei Jie , Feng Jingsheng , Xu Wenhe , Yang Bo , Tan Yiqing 
Published: November 2021

Abstract
Background

Red blood cell distribution width (RDW) and the platelet-to-lymphocyte ratio (PLR) are associated with different types of prognoses in critically ill patients. But, the value of RDW and PLR in predicting the occurrence of acute kidney injury (AKI) in critically ill patients are unknown. The purpose of the study was to explore the associations of RDW and PLR with AKI incidence.
Methods

Among 1500 adult patients in the intensive care unit (ICU) between January 2016 and December 2019 were enrolled, we examined the associations of baseline RDW and PLR with the risk of AKI development using logistical analysis. In addition, we explored the value of RDW and PLR in predicting in-hospital mortality.
Results

The study participants included 951 men and 549 women, aged 60.1±16.14 years. The subjects had a mean RDW of 14.65±2.14% and a mean PLR of 188.16±129.2. Overall, 615 (41%) patients were diagnosed with AKI. There were remarkable differences in RDW and the PLR between the AKI and non-AKI groups (P<0.001). After adjustment, the association of RDW with AKI development risk strengthened (OR: 1.28, 95% CI: 1.19-1.36). Moreover, we divided the groups into two subgroups each; the high-RDW (≥14.045%) group had a high risk of developing AKI (OR=5.189, 95% CI: 4.088-6.588), while the high-PLR(≥172.067)group had a risk of developing AKI (OR=9.11,95% CI:7.09-11.71). The areas under the receiver operating characteristic curves (AUCs) for the prediction of AKI incidence based on RDW and PLR were 0.780 (95% CI: 0.755-0.804) and 0.728 (95% CI:0.702-0.754) (all P< 0.001), with cut-off values of 14.045 and 172.067, respectively. Moreover, a higher RDW was associated with a higher rate of hospital mortality (OR: 2.907, 2.190-3.858), and the risk of in-hospital mortality related to PLR was 1.534 (95%CI: 1.179-1.995). The AUC for in-hospital mortality based on RDW was 0.663 (95%CI:0.628-0.698), while the AUC for in-hospital mortality based on the PLR was 0.552 (0.514-0.589).
Conclusions

A higher RDW related to a higher risk of the occurrence of AKI and in-hospital mortality in ICU.The PLR also showed predictive value for the occurrence of AKI but did not show any clear prediction value of in-hospital mortality.
Pan-cancer Analysis Reveals the Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC) Its Significance in Human Cancers
Authors: chen yujie , xu kuang , fei qiuwen , qin yongwei , deng zilong , chen jinliang , Hua minhui 
Published: November 2021

Abstract
BackgroundSNRPC is cloned on human chromosome 6p21.31, which encodes a special protein component of U1 snRNP granules. Although SNRPC played an important role in the pre-mRNA splicing starting and adjustment, but in the tumor biological function is still unknown.MethodThrough the pan-cancer analysis of SNRPC, and our data sets, phosphorylation and functional network analysis based on TCGA (Cancer Genome Map) and GEO (Integrated Gene Expression Database), also from the western blot, qRT-PCR and CCK-8, cloning-forming experiment, scratch experiment to prove SNRPC biological function.ResultsSNRPC is related to the regulation of RNA, shear, and protease signals and has an important effect on ovarian cancer prognosis. Through a series of biological information data mining and basic experiments, we found that SNRPC plays an important role in the proliferation and migration of ovarian cancer. SNRPC expression is positively correlated with the immersion of CD4+T cells, macrophages, and neutrophils (p< 0.05), as obtained through the TIMER database (Tumor Immunological Assessment Resources) database. ConclusionOur pan-cancer research provides SNRPC in different tumors, especially the relatively comprehensive understanding of the carcinogenic potential of ovarian cancer.
Influence of the First-wave COVID-19 Pandemic on Emergency Acute Coronary Syndrome: a Multicenter Retrospective Study From a Non-epicenter Region
Authors: Mao Qi , Zhao Jian-Hua , Chen Jian-Fei , Xu Qiang , Xu Zhong-Lin , Yu Yang , Zhang Ying , Li Qiang , Pang Xiao-Hua , Li Zheng-Gong , Ran Bo-Li , Zhang Zhi-Hui , Li Zhi-Feng , Zeng Chun-Yu , Tong Shi-Fei , Jin Jun , Huang Lan , Zhao Xiao-Hui 
Published: November 2021

Abstract
Background: The COVID-19 pandemic presented severe challenges to emergency practice of acute coronary syndrome (ACS). However, poor evidence was shown on ACS in a non-hot-spot region. We sought to clarify the influence of the first-wave COVID-19 pandemic on emergency ACS from a non-epicenter region.Methods: This retrospective multicenter study was conducted in emergency ACS patients during the pandemic (from 2020-01-23 to 2020-03-29) and the ones during the same period in 2019. Clinical characteristics, timeline parameters and treatment strategies were compared between different groups. Association of the pandemic with non-invasive therapy was further assessed.Results: Compared with 2019, ACS had a drop in admission (267 cases vs. 475 cases) and invasive therapy (140 cases vs. 318 cases). Also, process delays were detected including the period from symptom onset to first medical contact (S-to-FMC, 5h vs. 2.5h), the period from FMC to electrocardiogram (ECG) completed (8min vs. 4min) and the period from FMC to dual antiplatelet therapy (FMC-to-DAPT, 25min vs. 19min). Primary percutaneous coronary intervention (PPCI) decreased by 54.9% in STEMI and early invasive therapy decreased by 59.2% in NSTE-ACS. The proportion of invasive therapy in NSTE-ACS decreased more than in STEMI (16.9% vs. 10.1%) with longer process delay. The pandemic was associated with increased non-PPCI in STEMI (OR=1.707, 95%CI 1.082-2.692, P=0.021) and elevated medication in NSTE-ACS (OR=2.029, 95%CI 1.268-3.247, P=0.003), respectively. Conclusion: Even in a non-epicenter region, the first-wave COVID-19 pandemic caused a significant reduction of invasive therapy and evident process delays in emergency ACS.
Nanozyme-Natural Enzymes Cascade Catalyze Cholesterol Consumption and Reverse Cancer Multidrug Resistance
Authors: Du Bin , Zheng Mei , Huang Jingshu , Jiao Qingqing , Bai Yimeng , Zhao Mengmeng , Zhou Jie 
Published: November 2021

Abstract
Multidrug resistance is still a major obstacle to cancer treatment. The most studies are to inhibit the activity of the drug transporter P-glycoprotein (P-gp), but the effect is not ideal. Herein, a nanosystem was built based on cascade catalytic consumption of cholesterol. Cholesterol oxidase (natural enzyme, COD) was immobilized on the carrier (NH2-MIL-88B, MOF) through amide reaction, COD catalyzed the consumption of cholesterol, the reaction product H2O2 was further produced by the MOF with its peroxidase-like activity to produce hydroxyl radicals (•OH) with killing effect. Due to the high expression of CD44 receptor on the surface of tumor cells, we encapsulated chondroitin sulfate gel shell (CS-shell) with CD44 targeting and apoptosis promoting effect on the surface of DOX@MOF-COD nanoparticles, which can accurately and efficiently deliver the drugs to the tumor site and improve the effect of reversing drug resistance. Taking drug-resistant cell membrane as "breakthrough", this paper will provide a new idea for reversing multidrug resistance of tumor.
Experiencing Eight Psychotherapy Approaches Devoted to Eating Disorders in a Single Day Workshop Increases Insight and Motivation to Engage in Care
Authors: Scanferla Elisabetta , Pachoud Bernard , Gorwood Philip 
Published: November 2021

Abstract
PurposeFor patients with eating disorders (EDs), early engagement in care is usually considered as a positive prognostic factor. The aim of the present study is to investigate how a single-day intervention devoted to an early experiential exposure to a variety of psychotherapy approaches, supports transition to specialised care and commitment to change in patients with EDs.MethodsOne hundred and sixty-nine outpatients newly diagnosed with an ED took part in a single-day workshop for groups of up to 10 patients, where they discovered and experienced eight psychotherapeutic approaches. Motivation to change care, and level of insight were assessed at baseline and 10 days after the intervention.ResultsMotivation and commitment to take active steps toward change (expressed by the “Committed Action” composite score) significantly improved after intervention (p<0.001), and a significant number of patients specifically moved from “contemplation” to “action” stage (p<0.001). The improvement of motivation to change is significantly associated to an increase of insight capacity (p<0.001), and this for almost all dimensions.ConclusionA single-day session devoted to experiencing a range of group psychotherapies increased patients’ insight and motivation to actively engage in care. Further studies including different factors and long-term outcomes evaluation may be necessary to better establish which aspects are specifically involved in patients' increased motivation for care and confirm potential longer-term benefits of this intervention.Level of evidenceLevel V: Opinions of respected authorities, based on descriptive studies, narrative reviews, clinical experience, or reports of expert committees.
Chronic Pain Alters Somatosensory Evoked Potentials and Paired-pulse Inhibition in Athlete
Authors: Yamashiro Koya , Siiya Kanako , Ikarashi Koyuki , Baba Yasuhiro , Fujimoto Tomomi , Ochi Genta , Otsuru Naofumi , Onishi Hideaki , Sato Daisuke 
Published: November 2021

Abstract
Injuries are inevitable for athletes, and when injuries end up causing chronic pain, they usually force athletes to withdraw from training. Chronic pain is known to be caused by plastic changes in the brain; thus, the purpose of this study was to assess the somatosensory evoked potential (SEP) and the paired-pulse inhibition (PPI) in athletes suffering from chronic pain as compared to pain-free athletes. Twenty track and field (T&F) athletes, that were also undergraduate students, were recruited for this study. These athletes (12 men; 8 women) were divided into two groups of 10 based on their self-reporting of actively experiencing chronic pain (defined as pain that persisted for more than 3 months) or not. Both SEP and PPI in the primary somatosensory cortex (SI) were elicited by constant current square-wave pulses (of 0.2 ms duration) that were delivered to the right median nerve by an electrical stimulator through a surface bar electrode with a cathode proximal. Paired-pulse stimulation was set at interstimulus intervals of 30 and 100 ms. Subjects were randomly presented with 1,500 single- and paired-pulse stimuli at 2 Hz. Our measurements demonstrated a trend toward a lower N20 and P25 amplitude as well as a disinhibition of the PPI_30 ms in the athletes suffering from chronic pain. These findings suggest that chronic pain may modulate excitatory and inhibitory function of the SI in athletes as well as in patients suffering from complex regional pain syndrome or fibromyalgia.
Chronic Alcohol Reduces Bone Formation Through Inhibiting Proliferation and Promoting Aging of Endothelial Cells in Type-H Vessels
Authors: Chen Ao , Li Xiaoting , Zhao Jingyu , Zhou Jiawen , Xie Chunfeng , Chen Haiyun , Wang Qiuyi , Wang Rong , Miao Dengshun , Li Jie , Jin Jianliang 
Published: November 2021

Abstract
Background: Chronic alcohol is one of the leading risk factors for male osteoporosis . Angiogenesis and osteogenesis coupled by type-H vessels coordinate the biological process of bone homeostasis to prevent osteopenia. It is unknown whether alcohol inhibits type-H-vessel-dependent bone formation. Aims: This study aimed to determine whether alcohol hampers proliferation and promotes aging of endothelial cells of type-H vessels, and whether alcohol inhibits the differentiation of bone marrow-mesenchymal stem cells (BM-MSCs) into osteoblasts through reducing the number and secretion of endothelial cells in type-H vessels. Materials and Methods: Two-month-old mice fed with alcohol liquid diet (28% of calories) or normal liquid diet for two months. The tibias were isolated and detected with X-ray and micro-CT. Paraffin-embedded or frozen tibial sections were prepared and used for immunohistochemical or immunofluorescence staining respectively in vivo . Human Umbilical Vein Endothelial Cells (HUVECs) were treated with different-concentrated alcohol for 12 hours. The conditioned medium of the above HUVECs cells was collected to culture human BM-MSCs, which were induced to differentiate into osteoblasts in vitro . Results: The alcoholic diet retarded the bone growth and lead to osteoporosis, impaired bone formation of osteoblasts, and decreased CD31 hi EMCN hi type-H-vessel formation through inhibiting proliferation and promoting aging of endothelial cells in mice. Alcohol treatment obviously increased the expression of p16, while significantly decreased the expression of Bmi-1, CDK6, Cyclin D, E2F1 and BMP2 compared to vehicle. Alcohol inhibited the differentiation of BM-MSCs into osteoblasts through reducing the BMP2 secretion of endothelial cells in type-H vessels. Conclusions: Alcoholic diet impaired CD31 hi EMCN hi type-H-vessel formation through inhibiting proliferation and promoting aging of endothelial cells via Bmi-1/p16 signaling, and inhibited the differentiation of BM-MSCs into osteoblasts through reducing the BMP2 secretion of endothelial cells in type-H vessels. It provides a basis for developing a new treatment strategy targeting aging endothelial cells of type-H-vessel to prevent alcoholic osteopenia.
CXCL13 Expressed on Inflamed Cerebral Blood Vessels Recruit IL-21 Producing TFH Cells to Damage Neurons Following Stroke.
Authors: Rayasam Aditya , Kijak Julie A. , Kissel Lee , Kim Taehee , Hsu Martin , Joshi Dinesh , Laaker Collin J. , Cismaru Peter , Lindstedt Anders , Kovacs Krisztian , Vemuganti Raghu , Chiu Shing Yan , Priyathilaka Thanthrige Thiunuwan , Sandor Matyas , Fabry Zsuzsanna 
Published: November 2021

Abstract
Background: Ischemic stroke is a leading cause of mortality worldwide, largely due to the inflammatory response to brain ischemia during post-stroke reperfusion. Despite ongoing intensive research, there have not been any clinically approved drugs targeting the inflammatory component to stroke. Preclinical studies have identified T cells as pro-inflammatory mediators of ischemic brain damage, yet mechanisms that regulate the infiltration and phenotype of these cells are lacking. Further understanding of how T cells migrate to the ischemic brain and facilitate neuronal death during brain ischemia can reveal novel targets for post-stroke intervention.Methods: To identify the population of T cells that produce IL-21 and contribute to stroke, we performed transient middle cerebral artery occlusion (tMCAO) in mice and performed flow cytometry on brain tissue. We also utilized immunohistochemistry in both mouse and human brain sections to identify cell types and inflammatory mediators related to stroke-induced IL-21 signaling. To mechanistically demonstrate our findings, we employed pharmacological inhibitor anti-CXCL13 and performed histological analyses with Cresyl violet to evaluate its effects on brain infarct damage. Finally, to evaluate cellular mechanisms of stroke, we exposed mouse primary neurons to oxygen glucose deprivation (OGD) conditions with or without IL-21 and measured cell viability, caspase activity and JAK/STAT signaling.Results: Flow cytometry on brains from mice following tMCAO identified a novel population of cells IL-21 producing CXCR5+ CD4+ ICOS-1+ T follicular helper cells (TFH) in the ischemic brain early after injury. We observed augmented expression of CXCL13 on inflamed brain vascular cells and demonstrated that inhibition of CXCL13 protects mice from tMCAO by restricting the migration and influence of IL-21 producing TFH cells in the ischemic brain. We also illustrate that neurons express IL-21R in the peri-infarct regions of both mice and human stroke tissue in vivo. Lastly, we found that IL-21 acts on mouse primary ischemic neurons to activate the JAK/STAT pathway and induce Caspase 3/7 mediated apoptosis in vitro. Conclusion: These findings identify a novel mechanism for how pro-inflammatory T cells are recruited to the ischemic brain to propagate stroke damage and provide a potential novel therapeutic target for stroke.
The Impact of Catheter Removal Time on Urinary Continence and Overactive Bladder Symptoms After Robot-Assisted Radical Prostatectomy: A Retrospective Analysis of Consecutive 250 Cases From a Single Institution
Authors: Hao Han , Chen Xu , Liu Yue , Si Longmei , Chen Yuke , Zhang Meng , Yu Wei , Song Yi , Wu Shiliang , Zhang Zhongyuan , Zhao Zheng , Shen Cheng , Han Wenke 
Published: November 2021

Abstract
Background After radical prostatectomy, the optimal length of postoperative catheterization time remains to be determined. This study investigates the impact of catheter removal time on urinary continence and overactive bladder symptoms after robot-assisted radical prostatectomy (RARP).Methods Two hundred and fifty consecutive patients underwent RARP by a single surgeon between November 2020 and May 2021. Time to catheter removal was categorized into 7, 10, and ≥ 14 days. Continence was defined as no more than 1 pad used or no more than 20 grams of urine leakage per 24 hours. The patients' continence rates and overactive bladder symptom score (OABSS) were assessed at 48 hours, 1 week, 4 weeks, 12 weeks, and 24 weeks after catheter removal.Results Overall, continence rates were 36% 48 hours after catheter removal, 55.6% 1 week, 74.8% 4 weeks, 98.4% 12 weeks, and 100% 24 weeks after catheter removal. The median time to regain continence was 1 week. After stratification according to catheterization time, no significant difference in continence rates was found between different groups at each time point after catheter removal. Longer catheterization was not an independent predictor of continence recovery (10 days: OR 0.985, 95% CI 0.689-1.409, p = 0.936; ≥14 days: OR 1.194, 95% CI 0.869-1.642, p = 0.274). The presence of diabetes was associated with worse continence outcomes (OR 1.535, 95% CI 1.105-2.132, p = 0.011). The mean OABSS of patients in the continent group were significantly lower than the incontinent group at 48 hours, 1 week, and 4 weeks after catheter removal. No significant difference in OABSS was found between different catheterization time groups at each time point after catheter removal.Conclusions Our results demonstrated that different catheterization time (7 days, 10 days, ≥14 days) is not associated with short-, intermediate-, long-term continence outcomes or overactive bladder symptoms.
PALT (Partial Allogeneic Limbal Transplantation): A New Surgical Technique For Limbal Stem Cell Deficiency
Authors: Kesper Christiane , Viestenz Anja , Hammer Thomas , Heinzelmann Joana , Foja Sabine , Viestenz Arne 
Published: November 2021

Abstract
PurposeLimbal stem cell deficiency (LSCD) is a rare but extremely relevant disease of the eye. LSCD patients often require a variety of surgical procedures, including keratoplasty in some cases. However, the outcome of these surgeries, including opacification and revascularization, is often frustrating due to LSCD relapse.MethodsWe developed a new surgical technique for the treatment of LSCD in which partial allogenic limbal transplantation (PALT) is carried out as part of penetrating keratoplasty (PK). After the PK, 1-8 slices from the limbal tissue of the donor graft are prepared and placed under the double running sutures attaching the corneal graft. This procedure was performed on 14 patients with LSCD, caused by severe ocular burn in 5 cases and by infection in 9. Between one and eight limbal transplants were used depending on the extension of the LSCD. ResultsAll 14 patients showed stable or increased visual acuity after the PALT surgery compared to their preoperative visual acuity. All of the grafts were integrated into the superficial corneal layers without progression of corneal vascularization beyond the limbal grafts. The median follow-up period was 12 months on average.ConclusionThe PALT method seems to be a promising surgical procedure for the treatment of patients with LSCD. It can be properly carried out in the context of keratoplasty and does not require a separate donor tissue. The PALT grafts may offer the possibility of constructing a new limbal region, resulting in stable or even increased visual acuity and the absence of corneal vascularization.