bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide association study reveals the genes associated with the leaf inclusion contents in Chinese medical treeEucommia ulmoides
Authors: Liu Yong-Cheng , Peng Xiao-Xi , Lu Yan-Bing , Wu Xue-Xian , Chen Lin-Wu , Feng Hong 
Published: January 2021

ABSTRACTEucommia ulmoides is an economic tree that can biosynthesize secondary metabolites with pharmacological functions. Genetic basis of biosynthesis of these compounds is almost unknown. Therefore, genomic-wide association study was performed to exploit the genetic loci maybe involved in biosynthetic pathways of 5 leaf inclusions (aucubin, chlorogenic acid, gutta-percha, polyphenols, total flavonoids). It was shown that contents of the 5 leaf metabolites have a wide variation following normal distribution. A total of 2 013 102 single nucleotide polymorphism (SNP) markers were identified in a population containing 62 individual clones. Through genome-wide association study analysis, many SNP loci were identified perhaps associated with phenotypes of the leaf inclusions. Higher transcriptional levels of the candidate genes denoted by significant SNPs in leaves suggested they may be involved in biosynthesis of the leaf inclusions. These genetic loci provide with invaluable information for further studies on the gene functions in biosynthesis of the leaf inclusions and selective breeding of the plus trees.
The vacuolar amino acid transport system is a novel, direct target of GATA transcription factors
Authors: Sato Akane , Kimura Takumi , Hondo Kana , Kawano-Kawada Miyuki , Sekito Takayuki 
Published: January 2021

ABSTRACT
In Saccharomyces cerevisiae, Avt4 exports neutral and basic amino acids from vacuoles. Previous studies have suggested that the GATA transcription factors, Gln3 and Gat1, which are key regulators that adapt cells in response to changes in amino acid status, are involved in the AVT4 transcription. Here, we show that mutations in the putative GATA-binding sites of the AVT4 promoter reduced AVT4 expression. Consistently, a chromatin immunoprecipitation (ChIP) assay revealed that Gat1-Myc13 binds to the AVT4 promoter. Previous microarray results were confirmed that gln3∆gat1∆ cells showed a decrease in expression of AVT1 and AVT7, which also encode vacuolar amino acid transporters. Additionally, ChIP analysis revealed that the AVT6 encoding vacuolar acidic amino acid exporter represents a new direct target of the GATA transcription factor. The broad effect of the GATA transcription factors on the expression of AVT transporters suggests that vacuolar amino acid transport is integrated into cellular amino acid homeostasis.
Hyaluronidase and degranulation inhibitors from the edible roots of Oenanthe javanica including seric acids F and G that were obtained by heating
Authors: Murata Toshihiro , Katagiri Tatsuo , Osaka Masaaki , Yamauchi Shohei , Yoshimura Kenshi , Kawada Manami , Fujii Yu , Suzuki Yuka , Sasaki Kenroh 
Published: January 2021

ABSTRACT
Oenanthe javanica is a vegetable grown in East Asia and Australia in which the roots and aerial parts are boiled together to make certain traditional dishes. Nineteen compounds (1-19) were isolated from O. javanica roots and the chemical structures of 2 new norlignans were determined. The inhibitory effects of the compounds on hyaluronidase and degranulation in RBL-2H3 cells were evaluated to determine antiallergic and antiinflammation activities. Saponins (2-4) and the new norlignan seric acid G (12) were among the active compounds identified. Seric acid G (12), a methoxy derivative of seric acid F (11), was obtained as an interconverting mixture of 3:1 trans-cis isomers. Seric acids F and G (11, 12) were derived from seric acids C (10) and E, respectively, by decarboxylation and dehydration reactions that occurred during heating. It was confirmed by HPLC analysis that all eleven of the O. javanica cultivars contained seric acid C (10).
MetW regulates the enzymatic activity of MetX in Pseudomonas
Authors: Hasebe Fumihito 
Published: January 2021

ABSTRACT
Methionine is a canonical amino acid. The protein MetX is a homoserine O-acyltransferase utilized in the methionine biosynthetic pathway. The metW gene is found adjacent to the metX gene in some bacteria, but its functions are unclear. In this study, I focused on the function of MetW and MetX from Pseudomonas aeruginosa (PaMetW and PaMetX). I demonstrated that PaMetW interacted with and activated the homoserine O-succinyltransferase (HST) activity of PaMetX. Furthermore, I elucidated that the HST activity of PaMetX in complex with PaMetW was inhibited by the addition of S-adenosyl-l-homocysteine (SAH), although PaMetX alone showed no feedback inhibition. Since PaMetW possesses a glycine-rich sequence annotated as a SAM/SAH binding site, I also investigated the relationship between this glycine-rich sequence and the inhibition caused by SAH. I revealed that alanine mutation of PaMetW Gly24 reduced the inhibitory effect of SAH. These results suggest that MetW is a regulatory protein of MetX.
Long noncoding RNA ZFAS1 suppresses chondrocytes apoptosis via miR-302d-3p/SMAD2 in osteoarthritis
Authors: Li Jian , Liu Mingting , Li Xianrang , Shi Hui , Sun Shui 
Published: January 2021

ABSTRACT
Osteoarthritis (OA) seriously affects people's quality of life due to joint pain, stiffness, disability, and dyskinesia worldwide. Long noncoding RNA zinc finger antisense 1 (ZFAS1) is downregulated and tightly associated with proliferation, migration, apoptosis, and matrix synthesis of chondrocyte in OA. However, the molecular mechanisms of ZFAS1 in OA remain unknown. The expression correlation between ZFAS1, miR-302d-3p, and SMAD2 in OA tissues was analyzed by Pearson correlation analysis. ZFAS1 was a lower expression, and expedited proliferation and repressed apoptosis of chondrocytes. MiR-302d-3p was a direct target of ZFAS1. MiR-302d-3p hindered proliferation and facilitated apoptosis of chondrocytes. MiR-302d-3p partially reversed the effect of ZFAS1 on proliferation and apoptosis of chondrocytes. SMAD2 was positively regulated by the ZFAS1/miR-302d-3p. MiR-302d-3p-mediated proliferation and apoptosis were partly abrogated by targeting SMAD2. ZFAS1 promoted chondrocytes proliferation and repressed apoptosis possibly by regulating miR-302d-3p/SMAD2 axis, providing a potential target for OA treatment.
Consistency in supplemental food availability affects the space use of wintering birds
Authors: Mady Rachael P , Hochachka Wesley M , Bonter David N 
Published: January 2021

AbstractAcross the globe, millions of people feed wild birds and this practice has profound implications for many bird species. To better understand the effects of this supplemental feeding, many researchers have compared birds with access to inexhaustible supplemental food to those without access. However, the consistency of supplemental food availability varies with peoples’ provisioning habits because some people fill their feeders daily, while others do so sporadically. As the consistency of food availability changes, a bird’s foraging strategy, including its use of space, should change. To determine how space use varies with the consistency of supplemental food availability, we surveyed three species with access to experimental feeders that provided constant, pulsed, or no access to food. We conducted these surveys at two locations—near and far from the feeder—within nine sites to determine differences in space use among and within sites. Access to supplemental food, regardless of feeding regime, anchored the movements of each species near the feeders. However, the different feeding regimes had different effects on space use. Birds with constant access to supplemental food were continually anchored near the feeders, while the birds with pulsed access were temporarily anchored near the feeders. In one of three species (black-capped chickadee), birds concentrated in larger numbers near feeders with pulsed access when food was available compared to near feeders with constant access. Supplemental feeders act as spatial anchors but do so in different ways across species and feeding regimes with potentially varying implications for survival and population dynamics.
Determinants of extortion compliance: Empirical evidence from a victimization survey
Authors: Estévez-Soto Patricio R 
Published: January 2021

AbstractThis article focuses on the situational-, victim- and area-level determinants of extortion compliance. Extortion, a quintessential organized crime, is one of the most common crimes in Mexico. However, compliance with extortion demands is relatively rare. Previous research suggests that compliance with extortion depends on the perceived risk of punishment for non-compliance. However, most research has been theoretical or experimental. The article offers empirical evidence of patterns of extortion compliance based on data from a large commercial victimization survey conducted in Mexico. Findings suggest that situational factors (extortion type, the presence of weapons and number of offenders) are the main determinants of extortion compliance. Victim- and area-level variables have comparatively smaller effects. Implications for research and practice are discussed.
A Thorough HPLC Assay of Quaternary Veterinary Formulation coupled with Environmental Assessment Tool
Authors: Michael Adel M , Fayez Yasmin M , Monir Hany H , Nessim Christine K 
Published: January 2021

Abstract
A new and accurate reversed phase HPLC method with UV detection has been established for any veterinarian analyst for simultaneous determination of a veterinary quaternary mixture of sulphadimidine sodium (SDS), sulphaquinoxaline sodium (SQS), diaveridine (DVD) and vitamin K3 (VTK3) in their formulation. The stationary phase was SEA C18 column (250 × 4.6 mm i.d., 5 μm particle size) at 25°C with an isocratic mode, using a mobile phase containing a mixture of methanol:acetonitrile:distilled water in the ratio of (20:20:60, by volume). The flow rate was 0.8 mL min−1, and UV detection was performed at 230 nm. The HPLC assay was coupled with Environmental Assessment Tool (EAT), which represents a simple and proficient approach for profiling the greenness of the method. This takes into consideration the environmental, health and safety issues for all solvents that involved in the chromatographic method and calculates a total score that can be used for comparison of the greenness of different methods. The method was found to be linear over (0.5–30) μg/mL for all cited drugs with mean percentage recoveries (99.56 ± 1.141) for VTK3, (99.56 ± 1.056) for DVD, (99.62 ± 1.482) for SDS and (99.52 ± 1.205) for SQS. The results were statistically compared with those of the official and reported methods; using Student’s t-test and F-test, showing no significant difference with respect to accuracy. Specificity of the applied method was assessed by analyzing the laboratory-prepared mixtures. The developed method was validated according to ICH guidelines. The proposed methodology can be applied for rapid routine assay of this combination.
Chemical Profiling and Antioxidant Evaluation ofPaeonia lactifloraPall. “Zhongjiang” by HPLC–ESI–MS Combined with DPPH Assay
Authors: Wu Yichao , Jiang Yuanyuan , Zhang Li , Zhou Jing , Yu Yan , Zhou Yonghong , Kang Tairan 
Published: January 2021

AbstractPaeonia lactiflora Pall. “Zhongjiang” is one of the four major medicinal P. lactiflora plants in China. In this research, a high-performance liquid chromatography (HPLC)–diode array detector (DAD)–electrospray ionization–mass spectrometry method was established to identify various components in the extracts of P. lactiflora “Zhongjiang” (root extract or RE, stem and leaf extract or SLE and flower extract or FE). A total of 40 compounds, including 19 monoterpenoid glycosides, five tannins, 10 phenolic acids and their esters, and six other compounds, were determined or temporarily inferred from RE (35 species), SLE (20 species) and FE (15 species). Antioxidant evaluation indicates among the monomer compounds, catechin, gallic acid and ethyl gallate showed strong antioxidant activity close to vitamin C, ascorbic acid (Vc). Paeoniflorin, albiflorin, benzoylpaeoniflorin and 6′-O-benzoylalbiflorin had certain antioxidant activities, which were much lower than Vc. Furthermore, 19, 15 and 15 antioxidant-reactive components were screened from RE, SLE and FE by using the 1,1-diphenyl-2- picrylhydrazyl (DPPH)–HPLC test results. Results indicated that the ethanol extracts of P. lactiflora “Zhongjiang” had strong antioxidant activity, and the antioxidant active material basis was mainly composed of phenolic acids and gallic acid tannins. The main components of P. lactiflora “Zhongjiang”, monoterpenoid glycosides, had weak antioxidant capacity. Paeonia lactiflora stems, leaves and flowers were good sources of antioxidants.
Enhancing the appeal of cardiac rehabilitation for women: development and pilot testing of a women-only yoga cardiac rehabilitation programme
Authors: Murphy Barbara M , Zaman Sarah , Tucker Kim , Alvarenga Marlies , Morrison-Jack Jenni , Higgins Rosemary , Le Grande Michael , Nasis Arthur , Jackson Alun C 
Published: January 2021

Abstract

Aims
Despite its demonstrated benefits, cardiac rehabilitation (CR) attendance, and completion is suboptimal, particularly in women. The aims of this study were (i) to develop and pilot test a women-only yoga-based CR programme (the Women’s Yoga CR Programme; WYCRP); (ii) to investigate programme acceptability; and (iii) to investigate attendance and completion of the WYCRP and continuation to Phase III community-based CR.

Methods and results
Women eligible for CR at a tertiary hospital in Melbourne, Australia were recruited. Over a 6-month period in 2019, they were offered the WYCRP or usual CR. The WYCRP involved attendance at a 1-h yoga session following the usual 1-h mixed-sex education session, over a 7-week period. Participants completed pre- and post-programme questionnaires and attended focus groups to assess programme acceptability. Rates of attendance, completion, and continuation for the WYCRP were recorded and compared to those for the standard CR programme offered in 2018. In total, 27 women were eligible for the study and attended the initial CR assessment. Of those, 22 (81%) attended the WYCRP, 1 (4%) attended usual CR, and 4 (15%) did not attend CR. Ratings of programme acceptability were consistently positive; qualitative comments highlighted both physical and emotional benefits. While attendance at the WYCRP was not significantly higher than for usual CR the previous year (81% vs. 76%; P = 0.40), CR completion, and continuation were (95% vs. 56%; P = 0.02, and 72% vs. 12%; P &lt; 0.001, respectively).

Conclusion
This pilot study has demonstrated that women-only yoga-based CR is appealing to women and can improve women’s CR completion and continuation. These preliminary findings support further development of women-only and yoga-based CR options.
Feature Selection Algorithms Enhance the Accuracy of Frailty Indexes as Measures of Biological Age
Authors: Kim Sangkyu , Fuselier Jessica , Welsh David A , Cherry Katie E , Myers Leann , Jazwinski S Michal 
Published: January 2021

Abstract
Biological age captures some of the variance in life expectancy for which chronological age is not accountable, and it quantifies the heterogeneity in the presentation of the aging phenotype in various individuals. Among the many quantitative measures of biological age, the mathematically uncomplicated frailty/deficit index is simply the proportion of the total health deficits in various health items surveyed in different individuals. We used 3 different statistical methods that are popular in machine learning to select 17–28 health items that together are highly predictive of survival/mortality, from independent study cohorts. From the selected sets, we calculated frailty indexes and Klemera–Doubal’s biological age estimates, and then compared their mortality prediction performance using Cox proportional hazards regression models. Our results indicate that the frailty index outperforms age and Klemera–Doubal’s biological age estimates, especially among the oldest old who are most prone to biological aging-caused mortality. We also showed that a DNA methylation index, which was generated by applying the frailty/deficit index calculation method to 38 CpG sites that were selected using the same machine learning algorithms, can predict mortality even better than the best performing frailty index constructed from health, function, and blood chemistry.
Protecting private collections of paintings in France in the nineteenth century
Authors: Jouves Barbara 
Published: January 2021

Abstract
Between 1789 and 1870, the ways in which works of art and collections as a whole were to be preserved were debated at length in France by collectors and by a wide array of those with a professional interest. They had a common aim: to prevent works from alteration and degradation owing to their treatment and method of display in private collections. The promotion of such protective measures at a time when private apartments were becoming increasingly accessible to visitors confirms that an awareness of the idea of common heritage was beginning to emerge. The environment in which paintings were kept was a source of major concern for the amateur, especially when it came to protecting works of art within his private apartments or in the course of travel. In attempting to address these problems, several patents were filed in France that sought to promote treatments aimed at preserving important works in private ownership, and a number of strategies were put forward for the restoration and protection of works of art, the treatment of varnishes and the hanging of paintings in domestic spaces. Patents registered for a number of inventions enable some of these preservation processes to be identified in detail.
Communication About Medication by Providers-Adolescent and Young Adult Version: Confirmatory Factor Analyses
Authors: Feldman Estée C H , Durkin Lindsay K , Bugno Lindsey , Balistreri Kathryn A , Davies W. Hobart , Miller Steven A , Greenley Rachel Neff 
Published: January 2021

Abstract

Objective
To replicate the factor structure of a patient-report measure of provider communication about key medication prescription information, the Communication about Medication by Providers-Adolescent and Young Adult (CAMP-AYA) Version. We evaluated whether the 15-item, two-factor structure identified previously could be replicated via confirmatory factor analysis, and we also examined fit of unidimensional and bifactor models. Associations of CAMP-AYA Total and Factor Scores with provider satisfaction and select patient and medication characteristics were also examined.

Methods
Participants were 739 AYA (ages 18–25) who completed the CAMP-AYA, a provider satisfaction rating, and provided demographic and medication information.

Results
The bifactor model was best fitting (χ2 [75] = 689.60, p &lt; .0001; root mean squared error of approximation = 0.11, 90% CI [0.10, 0.11]; Comparative Fit Index = 0.98; Tucker–Lewis Index = 0.98; Standardized Root Mean Square Residual Index = 0.02). Internal consistency reliabilities for Total and Factor Scores were high (αs &gt; .89) and Total and Factor Scores were associated with provider satisfaction (ps &lt; .001). CAMP-AYA scores varied as a function of type of prescription (short vs. long term; new vs. refill), with higher scores reported in the context of long term (&gt;30-day course) or refilled prescriptions (ps &lt; .007) in most cases.

Conclusions
This study provides additional support for the reliability of the CAMP-AYA as a tool to assess AYA perceptions of provider key information coverage about medication prescriptions.
A natural antisense transcript acts as a negative regulator for the maize drought stress response gene ZmNAC48
Authors: Mao Yan , Xu Jie , Wang Qi , Li Guobang , Tang Xin , Liu Tianhong , Feng Xuanjun , Wu Fengkai , Li Menglu , Xie Wubing , Lu Yanli 
Published: January 2021

Abstract
Although plant-specific NAC transcription factors play crucial roles in response to abiotic stress, few reports describe the regulation of NAC genes in maize (Zea mays) by the cis-natural antisense transcripts (cis-NATs). In this study, 521 NAC genes from Gramineae were classified, of which 51 NAC genes contained cis-NATs. ZmNAC48 and cis-NATZmNAC48 co-localized to the same cell nucleus, and both transcripts responded to drought stress. Arabidopsis plants overexpressing ZmNAC48 had improved drought tolerance, lower rate of water loss, enhanced stomatal closure, and higher rates of survival. Transient expression in both maize protoplasts and tobacco leaves indicated that cis-NATZmNAC48 reduced ZmNAC48 expression. Western blotting and ribosome profiling analyses confirmed that cis-NATZmNAC48 lacked protein coding potential. Furthermore, the cis-NAT-derived small-interfering RNAs (nat-siRNAs) generated from the overlapping regions of ZmNAC48 and cis-NATZmNAC48 were detected in maize and transgenic Arabidopsis. Cis-NATZmNAC48 overexpressing maize showed higher water loss rate, increased stomatal opening, and had more dead leaves. Expression of ZmNAC48 and nat-siRNA was decreased in these plants. Taken together, our study indicates that both ZmNAC48 and cis-NATZmNAC48 are involved in plant drought stress responses, and that the double-stranded RNA-dependent mechanism is involved in the interaction between cis-NATZmNAC48 and ZmNAC48. Additionally, cis-NATZmNAC48 may negatively regulate ZmNAC48 to affect stomatal closure of maize.
Completeness theorems for first-order logic analysed in constructive type theory
Authors: Forster Yannick , Kirst Dominik , Wehr Dominik 
Published: January 2021

AbstractWe study various formulations of the completeness of first-order logic phrased in constructive type theory and mechanised in the Coq proof assistant. Specifically, we examine the completeness of variants of classical and intuitionistic natural deduction and sequent calculi with respect to model-theoretic, algebraic, and game-theoretic semantics. As completeness with respect to the standard model-theoretic semantics à la Tarski and Kripke is not readily constructive, we analyse connections of completeness theorems to Markov’s Principle and Weak Kőnig’s Lemma and discuss non-standard semantics admitting assumption-free completeness. We contribute a reusable Coq library for first-order logic containing all results covered in this paper.