bluuebunny/crossref_metadata_2025_split huggingface dataset:
КОМП’ЮТЕРНО ОРІЄНТОВАНИЙ МЕТОД П’ЯТИ МОМЕНТІВ У РОЗРАХУНКАХ ЗГИНАННЯ СУДНОВИХ ПЕРЕКРИТТІВ
Authors: Luginin O.Ye. , Terlych S.V. , Korshykov R.Yu. 
Published: August 2021

Вступ. На ранніх стадіях проєктування суден, їх спуску зі стапеля, постанов- ці в док та в навчальному процесі можуть бути використані наближені методи оцінки загальної та місцевої міцності суден. Запропоновано нову версію удоско- наленого та універсалізованого методу п’яти моментів (М5М). Метою дослі- дження є розробка нової версії М5М для стержневої моделі згинання суднових перекриттів із вирішенням таких завдань: врахування в балочній моделі судно- вих перекриттів нерегулярності розміщення балок головного напряму (БГН) та перехресних балок (ПБ) при їх непризматичності, довільності граничних умов, розподіленні навантаження на розглядувані балки; врахування деформації зсуву в стінках балок перекриття; врахування полосового навантаження від спускових доріжок та докового опорного пристрою (ДОП) під час докування суден. Резуль- тати. Розроблено комп’ютерно орієнтований метод розрахунку згинання судно- вих перекриттів з урахуванням їх особливостей у проєктуванні суден, спуску зі стапелю та докуванні. Висновки. Розроблено алгоритм і розрахункову методику з розгляду задач розрахунку згинання перекриттів у процесі проєктування суден, їх спуску з поздовжнього похилого стапелю та постановки в сухий або плавучий доки. Реалізована удосконалена балочна модель оцінки згинання суднових пере- криттів на основі М5М, який поширений та універсалізований для практичних розрахунків з урахуванням таких факторів: нерегулярність розстановки балок перекриття, довільності їх граничних умов; розподіл навантаження між балка- ми обох напрямів і видів навантаження на перекриття; врахування деформації зсуву в стінках балок; врахування особливостей деформування днищових пере- криттів суден під час їх спуску з похилого поздовжнього стапелю і докування за рахунок розгляду полосового навантаження балок.
Relación entre la carga externa y las concentraciones de cortisol durante el microciclo competitivo en jugadores internacionales de fútbol
Authors: Aceña Angel , Pombal David Fombella , Alvarado Erick Sánchez , Golubev Denis Vyacheslavovich 
Published: August 2021

Objetivo: La hormona cortisol participa en situaciones de respuestas fisiológicas y se ve afectada por la demanda de carga de entrenamiento sometida al organismo, de esta manera se pretende conocer la correlación entre las concentraciones de cortisol medidas al final de microciclo competitivo con las demandas de carga externa en 20 jugadores internacionales.
Método: Durante la Copa Mundial de la Fédération Internationale de Football Association 2018 en Rusia, un equipo nacional participante optó por analizar las concentraciones de cortisol de los jugadores para poder establecer relaciones entre la carga externa acumulada en cada microciclo (distancia total, distancia entre 14-19 km/h, distancia entre 19-24 km/h, Distancia > 24 km/h, promedio metros/min y promedio máxima velocidad ) medida con dispositivos Global Positioning System durante cuatro microciclos completos y los niveles de cortisol el día antes de partido y en el día de partido.
Resultados: Entre los resultados obtenidos, se observa una correlación negativa moderada entre las concentraciones de cortisol medidos el el día antes de partido y el día de partido con distancia total acumulada en el microciclo (r= -0.44 y -0.36 respectivamente), así como correlaciones negativas moderadas entre el cortisol en el día antes de partido con la distancia acumulada cubierta entre 14-19 km/h. (r= -0.32)
Conclusiones: A la luz de los resultados, se observa como la carga externa de entrenamiento cubierta durante el microciclo, puede influir en las concentraciones de cortisol acumuladas al final del mismo, con especial énfasis en la distancia total acumulada.
Fuerza muscular inspiratoria dinámica y aptitud cardiorrespiratoria en función de edad y sexo
Authors: Roldán Ainoa , Blasco-Lafarga Nieves María , Cordellat Ana , Monteagudo Pablo , Gómez-Cabrera Mª Carmen , Blasco-Lafarga Cristina 
Published: August 2021

Objetivo: Analizar la relación entre fuerza inspiratoria y aptitud cardiorrespiratoria en un grupo de adultos mayores sanos y entrenados, atendiendo a edad y sexo.
Método: Estudio descriptivo, de corte transversal y prospectivo. De los 153 participantes en el programa de entrenamiento multicomponente EFAM-UV© y mayores de 60 años, 87 fueron evaluados de composición corporal, presión arterial, saturación de oxígeno, fuerza inspiratoria máxima y test de 6 minutos marcha. Como criterios de exclusión: fumador, padecer/haber padecido enfermedad respiratoria, cardíaca, neuromuscular o de la caja torácica, padecer/haber padecido deterioro cognitivo y/o llevar prótesis dental. 67 adultos mayores (73.02 ± 4.54 años) se consideraron para el análisis final. Los test se realizaron en tres días no consecutivos con una separación de entre 48 y 72 horas.
Resultados: Se confirman las diferencias atendiendo al sexo en ambas variables. La asociación moderada entre fuerza inspiratoria máxima y aptitud cardiorrespiratoria, medida a través del test de 6 minutos marcha, (r=0.468; p<0.005) se reduce al tener en cuenta las covariables edad (re=0.397; p<0.005) y sexo (rs=0.360; p<0.005), con menor R2 en las mujeres (0.113 vs. 0.188).
Conclusión: Estos resultados justifican la necesidad de evaluar y entrenar la musculatura inspiratoria en los adultos mayores, especialmente en las mujeres, con independencia de su nivel de aptitud física. Futuros estudios deben analizar qué factores pueden influir en la asociación entre la fuerza inspiratoria máxima y la aptitud cardiorrespiratoria (concretamente test de 6 minutos marcha) más allá de la edad y el sexo.
La socialización mejora la adherencia, pero no la fuerza, en programas de ejercicio físico con componente de fuerza en personas mayores
Authors: Cancela José María , Vila María Helena , Sánchez-Lastra Miguel Adriano , Varela Marínez Silvia 
Published: August 2021

Objetivo: Evaluar los efectos de realizar dos programas de ejercicio con componente de fuerza, uno de ejercicios individuales y otro por parejas, sobre la adherencia a los mismos y sus efectos en la independencia funcional.
Método: Sesenta y cuatro hombres y mujeres (60-90 años) participaron en dos programas de ejercicio, uno de entrenamiento de fuerza y otro de entrenamiento multicompontente. En uno, se realizaron ejercicios de forma individual y en el otro por parejas. Se evaluó la adherencia a los programas y la fuerza de tren superior e inferior, la movilidad funcional y la capacidad cardiorrespiratoria antes y después de los programas.
Resultados: La adherencia fue menor en al programa de fuerza individual (57.6%; 7 pérdidas) frente al multicomponente (83.7%; 2 pérdidas). Se hallaron mejoras significativas en el programa de ejercicio individual en la fuerza y movilidad funcional y empeoramientos significativos en el de ejercicio por parejas.
Conclusión: La realización de ejercicios de fuerza colectiva favoreció la adherencia al programa pero no se tradujo en beneficios significativos, en comparación con el grupo de fuerza individual.
Efectos del ejercicio en cirugía bariátrica
Authors: Cobos-Fernández Mar , González González Yoana , Alonso Calvete Alejandra , Da Cuña Carrera Iria 
Published: August 2021

Introducción: El objetivo de esta revisión es analizar la literatura científica más actual acerca de los efectos del ejercicio en pacientes obesos o sometidos a cirugía bariátrica.
Desarrollo: Se ha llevado a cabo una revisión de la literatura científica actual mediante una búsqueda sistematizada en PubMed, Scopus, Cinhal, Web Of Science y SPORTDiscus con los descriptores “bariatric surgery”, “exercise therapy” y “therapeutic exercise”, obteniendo finalmente 13 artículos.
Se observó que el ejercicio produce beneficios en cuanto a composición corporal como pérdida de peso, aumento de masa magra, masa muscular e insulina en sangre y favorece la actividad fibrinolítica. Además, produce un aumento de la capacidad aeróbica y, por tanto, una mejora de la condición física.
Conclusiones: Podemos concluir que el ejercicio es una herramienta efectiva en el tratamiento de pacientes con obesidad tanto antes como después de la cirugía.
Partial Inhibition of the 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase-3 (PFKFB3) Enzyme in Myeloid Cells Does Not Affect Atherosclerosis
Authors: Tillie Renée J. H. A. , De Bruijn Jenny , Perales-Patón Javier , Temmerman Lieve , Ghosheh Yanal , Van Kuijk Kim , Gijbels Marion J. , Carmeliet Peter , Ley Klaus , Saez-Rodriguez Julio , Sluimer Judith C. 
Published: August 2021

BackgroundThe protein 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) is a key stimulator of glycolytic flux. Systemic, partial PFKFB3 inhibition previously decreased total plaque burden and increased plaque stability. However, it is unclear which cell type conferred these positive effects. Myeloid cells play an important role in atherogenesis, and mainly rely on glycolysis for energy supply. Thus, we studied whether myeloid inhibition of PFKFB3-mediated glycolysis in Ldlr–/–LysMCre+/–Pfkfb3fl/fl (Pfkfb3fl/fl) mice confers beneficial effects on plaque stability and alleviates cardiovascular disease burden compared to Ldlr–/–LysMCre+/–Pfkfb3wt/wt control mice (Pfkfb3wt/wt).Methods and ResultsAnalysis of atherosclerotic human and murine single-cell populations confirmed PFKFB3/Pfkfb3 expression in myeloid cells, but also in lymphocytes, endothelial cells, fibroblasts and smooth muscle cells. Pfkfb3wt/wt and Pfkfb3fl/fl mice were fed a 0.25% cholesterol diet for 12 weeks. Pfkfb3fl/fl bone marrow-derived macrophages (BMDMs) showed 50% knockdown of Pfkfb3 mRNA. As expected based on partial glycolysis inhibition, extracellular acidification rate as a measure of glycolysis was partially reduced in Pfkfb3fl/fl compared to Pfkfb3wt/wt BMDMs. Unexpectedly, plaque and necrotic core size, as well as macrophage (MAC3), neutrophil (Ly6G) and collagen (Sirius Red) content were unchanged in advanced Pfkfb3fl/fl lesions. Similarly, early lesion plaque and necrotic core size and total plaque burden were unaffected.ConclusionPartial myeloid knockdown of PFKFB3 did not affect atherosclerosis development in advanced or early lesions. Previously reported positive effects of systemic, partial PFKFB3 inhibition on lesion stabilization, do not seem conferred by monocytes, macrophages or neutrophils. Instead, other Pfkfb3-expressing cells in atherosclerosis might be responsible, such as DCs, smooth muscle cells or fibroblasts.
Isolation and Identification of Isocoumarin Derivatives With Specific Inhibitory Activity Against Wnt Pathway and Metabolome Characterization of Lasiodiplodia venezuelensis
Authors: Pellissier Léonie , Koval Alexey , Marcourt Laurence , Ferreira Queiroz Emerson , Lecoultre Nicole , Leoni Sara , Quiros-Guerrero Luis-Manuel , Barthélémy Morgane , Duivelshof Bastiaan L. , Guillarme Davy , Tardy Sébastien , Eparvier Véronique , Perron Karl , Chave Jérôme , Stien Didier , Gindro Katia , Katanaev Vladimir , Wolfender Jean-Luc 
Published: August 2021

The Wnt signaling pathway controls multiple events during embryonic development of multicellular animals and is carcinogenic when aberrantly activated in adults. Breast cancers are dependent on Wnt pathway overactivation mostly through dysregulation of pathway component protein expression, which necessitates the search for therapeutically relevant compounds targeting them. Highly diverse microorganisms as endophytes represent an underexplored field in the therapeutic natural products research. In the present work, the objective was to explore the chemical diversity and presence of selective Wnt inhibitors within a unique collection of fungi isolated as foliar endophytes from the long-lived tropical palmAstrocaryum sciophilum. The fungi were cultured, extracted with ethyl acetate, and screened for their effects on the Wnt pathway and cell proliferation. The endophytic strainLasiodiplodia venezuelensiswas prioritized for scaled-up fractionation based on its selective activity. Application of geometric transfer from analytical HPLC conditions to semi-preparative scale and use of dry load sample introduction enabled the isolation of 15 pure compounds in a single step. Among the molecules identified, five are original natural products described for the first time, and six are new to this species. An active fraction obtained by semi-preparative HPLC was re-purified by UHPLC-PDA using a 1.7 µm phenyl column. 75 injections of 8 µg were necessary to obtain sufficient amounts of each compound for structure elucidation and bioassays. Using this original approach, in addition to the two major compounds, a third minor compound identified as (R)-(-)-5-hydroxymellein (18) was obtained, which was found to be responsible for the significant Wnt inhibition activity recorded. Further studies of this compound and its structural analogs showed that only 18 acts in a highly specific manner, with no acute cytotoxicity. This compound is notably selective for upstream components of the Wnt pathway and is able to inhibit the proliferation of three triple negative breast cancer cell lines. In addition to the discovery of Wnt inhibitors of interest, this study contributes to better characterize the biosynthetic potential ofL. venezuelensis.
Dynamics of SARS-CoV2 Infection and Multi-Drug Resistant Bacteria Superinfection in Patients With Assisted Mechanical Ventilation
Authors: Mazzariol Annarita , Benini Anna , Unali Ilaria , Nocini Riccardo , Smania Marcello , Bertoncelli Anna , De Sanctis Francesco , Ugel Stefano , Donadello Katia , Polati Enrico , Gibellini Davide 
Published: August 2021

ObjectiveTo investigate the presence of bacteria and fungi in bronchial aspirate (BA) samples from 43 mechanically ventilated patients with severe COVID-19 disease.MethodsDetection of SARS-CoV-2 was performed using Allplex 2019-nCoV assay kits. Isolation and characterisation of bacteria and fungi were carried out in BA specimens treated with 1X dithiothreitol 1% for 30 min at room temperature, using standard culture procedures.ResultsBacterial and/or fungal superinfection was detected in 25 out of 43 mechanically ventilated patients, generally after 7 days of hospitalisation in an intensive care unit (ICU). Microbial colonisation (colony forming units (CFU) &lt;1000 colonies/ml) in BA samples was observed in 11 out of 43 patients, whereas only 7 patients did not show any signs of bacterial or fungal growth. Pseudomonas aeruginosa was identified in 17 patients. Interestingly, 11 out of these 17 isolates also showed carbapenem resistance. The molecular analysis demonstrated that resistance to carbapenems was primarily related to OprD mutation or deletion. Klebsiella pneumoniae was the second most isolated pathogen found in 13 samples, of which 8 were carbapenemase-producer strains.ConclusionThese data demonstrate the detection of bacterial superinfection and antimicrobial resistance in severe SARS-CoV-2-infected patients and suggest that bacteria may play an important role in COVID-19 evolution. A prospective study is needed to verify the incidence of bacterial and fungal infections and their influence on the health outcomes of COVID-19 patients.
Identification of Natural Inhibitors Against SARS-CoV-2 Drugable Targets Using Molecular Docking, Molecular Dynamics Simulation, and MM-PBSA Approach
Authors: Kushwaha Prem Prakash , Singh Atul Kumar , Bansal Tanya , Yadav Akansha , Prajapati Kumari Sunita , Shuaib Mohd , Kumar Shashank 
Published: August 2021

The present study explores the SARS-CoV-2 drugable target inhibition efficacy of phytochemicals from Indian medicinal plants using molecular docking, molecular dynamics (MD) simulation, and MM-PBSA analysis. A total of 130 phytochemicals were screened against SARS-CoV-2 Spike (S)-protein, RNA-dependent RNA polymerase (RdRp), and Main protease (Mpro). Result of molecular docking showed that Isoquercetin potentially binds with the active site/protein binding site of the Spike, RdRP, and Mpro targets with a docking score of -8.22, -6.86, and -9.73 kcal/mole, respectively. Further, MS 3, 7-Hydroxyaloin B, 10-Hydroxyaloin A, showed -9.57, -7.07, -8.57 kcal/mole docking score against Spike, RdRP, and Mpro targets respectively. The MD simulation was performed to study the favorable confirmation and energetically stable complex formation ability of Isoquercetin and 10-Hydroxyaloin A phytochemicals in Mpro-unbound/ligand bound/standard inhibitor bound system. The parameters such as RMSD, RMSF, Rg, SASA, Hydrogen-bond formation, energy landscape, principal component analysis showed that the lead phytochemicals form stable and energetically stabilized complex with the target protein. Further, MM-PBSA analysis was performed to compare the Gibbs free energy of the Mpro-ligand bound and standard inhibitor bound complexes. The analysis revealed that the His-41, Cys145, Met49, and Leu27 amino acid residues were majorly responsible for the lower free energy of the complex. Drug likeness and physiochemical properties of the test compounds showed satisfactory results. Taken together, the study concludes that that the Isoquercetin and 10-Hydroxyaloin A phytochemical possess significant efficacy to bind SARS-Cov-2 Mpro active site. The study necessitates further in vitro and in vivo experimental validation of these lead phytochemicals to assess their anti-SARS-CoV-2 potential.
The Association of Left Atrial Appendage Morphology to Atrial Fibrillation Recurrence After Radiofrequency Ablation
Authors: Gong Shiyu , Zhou Jian , Li Bingyu , Kang Sheng , Ma Xiaoye , Cai Ying , Guo Yang , Hu Rui , Zhang Xumin 
Published: August 2021

Objective: The probability of late recurrent atrial fibrillation (AF) after radiofrequency ablation (RFA) has not yet been fully clarified. This study aims to study the association of left atrial appendage (LAA) morphology with AF recurrence after RFA.Methods: We retrospectively enrolled 84 patients (24 patients had persistent AF, 60 patients had paroxysmal AF) who underwent RFA in Shanghai East Hospital from June 2014 to May 2018. The mean follow-up of these patients was 618.6 days. According to preoperative transesophageal echocardiography (TEE), the morphology feature of LAA was classified and evaluated by two classification methods. The first method was divided into chicken-wing, windsock, cactus, and cauliflower, and the second method was divided into one lobe, two lobes, and multiple lobes. The correlation between morphological feature of LAA and the recurrence rate of AF after RFA was analyzed.Results: During follow-up, 12 patients (50%) and 10 patients (16.7%) had AF recurrence in persistent and paroxysmal AF, respectively. The LAA morphology was associated with the recurrence of AF after RFA with the chicken-wing highest recurrence risk (68.2%). The structure type of LAA was also related to the AF recurrence rate (p &lt; 0.01). Compared with one lobe and multiple lobes, two lobes (recurrence, 47.6%) were more likely associated with the recurrence of AF (p &lt; 0.02). Logistic regression analysis showed that the chicken-wing group had a higher risk of recurrence after RFA (OR = 8.13, p = 0.004), and the windsock group had a lower risk of recurrence (OR = 0.17, p = 0.002).Conclusion: The morphological feature of LAA is related to the recurrence risk of AF after RFA. LAA morphology assessment can predict the risk of AF recurrence.
Blood Pressure Response in Miners Exposed to Chronic Intermittent Hypoxia in Chile
Authors: Lang Morin , Paéz Valeria , Maj Giacomo , Silva-Urra Juan , Labarca-Valenzuela Cristián , Caravita Sergio , Faini Andrea , Cantuarias Javier , Perez Oscar , Bilo Grzegorz , Parati Gianfranco 
Published: August 2021

Introduction: Limited information is available on blood pressure (BP) behavior in workers exposed to chronic intermittent hypoxia (CIH), and even less is known regarding effects of CIH on 24-h ambulatory BP in those affected by arterial hypertension at sea level (SL). The aims of this study were to assess clinic and 24-h ambulatory BP at SL and at high altitude (HA; 3,870 m above SL) in workers exposed to CIH, and to compare BP response to HA exposure between normotensive and hypertensive workers.Methods: Nineteen normotensive and 18 pharmacologically treated hypertensive miners acclimatized to CIH were included, whose work was organized according to a “7 days-on−7 days-off” shift pattern between SL and HA. All measurements were performed on the second and seventh day of their HA shift and after the second day of SL sojourn.Results: Compared to SL, 24-h systolic BP (SBP) and diastolic BP (DBP) increased at HA [+14.7 ± 12.6 mmHg (p &lt; 0.001) and +8.7 ± 7.2 mmHg (p &lt; 0.001), respectively], and SBP nocturnal fall decreased consistently (−4.1 ± 9.8%; p &lt; 0.05) in all participants, with hypertensives showing higher nocturnal DBP than normotensives (p &lt; 0.05) despite the current therapy. Also, heart rate (HR) nocturnal fall tended to be reduced at HA. In addition, the 24-h SBP/DBP hypertension threshold of ≥130/80 mmHg was exceeded by 39% of workers at SL and by 89% at HA. Clinic HR, SBP, and DBP were significantly higher on the second day of work at HA compared with SL, the increase being more pronounced for SBP in hypertensives (p &lt; 0.05) and accompanied by, on average, mild altitude sickness in both groups. These symptoms and the values of all cardiovascular variables decreased on the seventh day at HA (p &lt; 0.05) regardless of CIH exposure duration.Conclusion: Long history of work at HA according to scheduled CIH did not prevent the occurrence of acute cardiovascular changes at HA during the first days of exposure. The BP response to HA tended to be more pronounced in hypertensive than in normotensive workers despite being already treated; the BP changes were more evident for 24-h ambulatory BP. Twenty-four-hour ABP monitoring is a useful tool for an appropriate evaluation of BP in CIH workers.
Effect of SYTL3-SLC22A3 Variants, Their Haplotypes, and G × E Interactions on Serum Lipid Levels and the Risk of Coronary Artery Disease and Ischaemic Stroke
Authors: Zheng Peng-Fei , Yin Rui-Xing , Cao Xiao-Li , Chen Wu-Xian , Wu Jin-Zhen , Huang Feng 
Published: August 2021

Background: The current study aimed to investigate the effects of synaptotagmin-like 3 (SYTL3) and solute carrier family 22 member 3 (SLC22A3) single nucleotide polymorphisms (SNPs) and gene-environment (G × E) interactions on blood lipid levels as well as the risk of coronary artery disease (CAD) and ischaemic stroke (IS) in the Southern Chinese Han population.Methods: The genetic makeup of 6 SYTL3-SLC22A3 SNPs in 2269 unrelated participants (controls, 755; CAD, 758 and IS, 756) of Chinese Han ethnicity was detected by the next-generation sequencing techniques.Results: The allele and genotype frequencies of the SYTL3 rs2129209 and SLC22A3 rs539298 SNPs were significantly different between the case and control groups. The SLC22A3 rs539298 SNP was correlated with total cholesterol (TC) levels in controls, the rs539298G allele carriers maintained lower TC levels than the rs539298G allele non-carriers. At the same time, the SLC22A3 rs539298 SNP interacted with alcohol consumption reduced the risk of CAD and IS. The SYTL3-SLC22A3 A-C-A-A-A-A, G-T-C-G-C-A and A-T-A-A-C-A haplotypes increased and the A-C-A-A-C-G haplotype reduced the risk of CAD, whereas the SYTL3-SLC22A3 A-C-A-A-A-A, G-T-C-G-A-G and A-T-A-A-C-A haplotypes increased and the A-C-A-A-A-G and A-C-A-A-C-G haplotypes reduced the risk of IS. In addition, several SNPs interacted with alcohol consumption, body mass index ≥ 24 kg/m2 and cigarette smoking to affect serum lipid parameters such as triglyceride, high-density lipoprotein cholesterol, TC, and apolipoprotein A1 levels.Conclusions: Several SYTL3-SLC22A3 variants, especially the rs539298 SNP, several haplotypes, and G × E interactions, were related to blood lipid parameters and the risk of CAD and IS in the Southern Chinese Han population.