bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single target acuity in the common sunfish (Lepomis gibbosus)
Authors: Spratte Caroline , Sandow Laura-Marie , Schnermann Julia S. , Hanke Frederike D. 
Published: September 2021

ABSTRACTVision contributes to foraging, territorial and reproductive behavior in sunfish. In these contexts, sunfish need to perceive single targets, such as prey items or body markings from either conspecifics or individuals of other sunfish species, from some distance. We determined the single target acuity of six common sunfish in a behavioral experiment to assess whether the visual abilities of sunfish correspond with behavioral observations or reactive distance measures, and thus assessed the limits of vision for the mentioned behaviors. Single target acuity for full-contrast single targets amounted to 0.17 deg (0.13–0.32 deg). When contrast was reduced to Weber contrasts of 0.67 and 0.41, single target acuity dropped to 0.34 deg (0.31–0.37 deg), and finally to 0.42 deg (0.34–0.54 deg). Single target acuity would thus allow common sunfish to perceive biologically relevant stimuli at reasonable distances even when contrast is reduced.
Effectiveness of interventions to support the transition home after acute stroke: a protocol for a systematic review
Authors: O'Callaghan Geraldine , Fahy Martin , Murphy Paul , Langhorne Peter , Galvin Rose , Horgan Frances 
Published: September 2021

Background Despite advances in the quality of acute stroke management, there are gaps in knowledge about effective support interventions to better manage the transition of care to home for patients with this complex condition. The goal of this systematic review is to explore the literature around support interventions available for patients as they navigate from acute hospital, rehabilitation or early supported discharge (ESD) services to independent living at home; and to establish if, in comparison with usual care or other comparative active interventions, support services offered to patients as they transition from acute hospital, inpatient rehabilitation/ESD to home, can achieve better patient and / or process outcomes. Protocol In September 2021, we will carry out, on electronic peer-reviewed databases, a comprehensive literature search based on a pre-defined search strategy, developed and conducted in collaboration with an Information Specialist. In an effort to identify all published trials we will perform citation tracking of included studies, check reference lists of relevant articles, review grey literature, and extend our search to google scholar. We will include randomised controlled trials (including cluster and quasi-randomisation) recruiting stroke patients transitioning to home, to receive either usual care or any support intervention designed to improve outcomes after stroke. The primary clinical outcome will be functional status. Two review authors will scrutinise trials, categorise them on their eligibility, and extract data. We will analyse the results for all trials and perform meta-analyses where possible. We will assess risk of bias for the included trials and use GRADE to assess the quality of the body of evidence. Patient and public involvement (PPI) engaged in the development of the research questions, and will participate in co-design of a strategy for dissemination of findings. Conclusions: The findings from this review will be used to identify knowledge gaps to direct future research.
Whole genome sequencing of two human rhinovirus A types (A101 and A15) detected in Kenya, 2016-2018
Authors: Luka Martha M. , Kamau Everlyn , de Laurent Zaydah R. , Morobe John Mwita , Alii Leonard K. , Nokes D. James , Agoti Charles N. 
Published: September 2021

Background: Virus genome sequencing is increasingly utilized in epidemiological surveillance. Genomic data allows comprehensive evaluation of underlying viral diversity and epidemiology to inform control. For human rhinovirus (HRV), genomic amplification and sequencing is challenging due to numerous types, high genetic diversity and inadequate reference sequences. Methods: We developed a tiled amplicon type-specific protocol for genome amplification and sequencing on the Illumina MiSeq platform of two HRV types, A15 and A101. We then assessed added value in analyzing whole genomes relative to the VP4/2 region only in the investigation of HRV molecular epidemiology within the community in Kilifi, coastal Kenya. Results: We processed 73 nasopharyngeal swabs collected between 2016-2018, and 48 yielded at least 70% HRV genome coverage. These included all A101 samples (n=10) and 38 (60.3%) A15 samples. Phylogenetic analysis revealed that the Kilifi A101 sequences interspersed with global A101 genomes available in GenBank collected between 1999-2016. On the other hand, our A15 sequences formed a monophyletic group separate from the global genomes collected in 2008 and 2019. An improved phylogenetic resolution was observed with the genome phylogenies compared to the VP4/2 phylogenies. Conclusions: We present a type-specific full genome sequencing approach for obtaining HRV genomic data and characterizing infections.
Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses
Authors: König Katja Marie Kjara , Jahun Aminu S. , Nayak Komal , Drumright Lydia N. , Zilbauer Matthias , Goodfellow Ian , Hosmillo Myra 
Published: September 2021

Human noroviruses (HuNoV) are the major cause of viral gastroenteritis worldwide. Similar to other positive-sense single-stranded RNA viruses, norovirus RNA replication requires the formation of a negative strand RNA intermediate. Methods for detecting and quantifying the viral positive or negative sense RNA in infected cells and tissues can be used as important tools in dissecting virus replication. In this study, we have established a sensitive and strand-specific Taqman-based quantitative polymerase chain reaction (qPCR) assay for both genogroups GI and GII HuNoV. This assay shows good reproducibility, has a broad dynamic range and is able to detect a diverse range of isolates. We used tagged primers containing a non-viral sequence for the reverse transcription (RT) reaction and targeted this tag in the succeeding qPCR reaction to achieve strand specificity. The specificity of the assay was confirmed by the detection of specific viral RNA strands in the presence of high levels of the opposing strands, in both RT and qPCR reactions. Finally, we further validated the assay in norovirus replicon-bearing cell lines and norovirus-infected human small intestinal organoids, in the presence or absence of small-molecule inhibitors. Overall, we have established a strand-specific qPCR assay that can be used as a reliable method to understand the molecular details of the human norovirus life cycle.
Concepções de enfermeiras egressas da residência acerca da interdisciplinaridade em Centros de Atenção Psicossocial
Authors: Alves Caroline Silva Batista , Espirito Santo Tiago Braga do , Casanova Edna Gurgel 
Published: September 2021

Objetivo: analisar as concepções das egressas da Residência de Enfermagem Psiquiátrica e Saúde Mental sobre o trabalho interdisciplinar em Centros de Atenção Psicossocial. Método: qualitativa-exploratória. Os dados foram coletados por entrevistas semiestruturadas com 04 participantes, entre julho a agosto de 2017. Utilizou-se a análise temática de Bardin. Aprovado pelo Comitê de Ética em Pesquisa. Resultados: Evidenciou-se o valor da integralidade do cuidado, produzida pelo compartilhamento de saberes. Destacam-se, contudo, os desafios atrelados à hierarquização, à valorização das competências profissionais específicas e à necessária revisão do processo formativo. Conclusão: A interdisciplinaridade é compreendida como fundamental para a integralidade do cuidado, mas vem encontrando dificuldades nos serviços e em seu desenvolvimento na formação lato sensu, devido a preservação do modelo biomédico. Aponta-se para a necessidade de implementação de espaços de educação permanente e revisão curricular dos cursos da saúde, produzindo reflexões que extrapolem a especificidade profissional.
O dever fundamental de não atentar contra o Estado democrático de direito: uma aproximação entre Jürgen Habermas e Thomas S. Kuhn e a extinção da Política Nacional de Participação Social
Authors: Viola Thiago , Pedra Adriano Sant’Ana 
Published: September 2021

O presente estudo tem como objetivo investigar se a extinção da Política Nacional de Participação Popular descumpre o dever fundamental de não atentar contra o Estado Democrático de Direito. No primeiro capítulo é apresentada a base teórica do artigo, que utiliza a epistemologia e o conceito de paradigma de Thomas S. Kuhn, reformulado como matriz disciplinar em suas obras pós-críticas, em diálogo com o paradigma procedimental da democracia discursiva habermasiana. No segundo capítulo, realiza-se a revisão de artigos científicos e da base normativo-política do dever fundamental e humano de respeitar a ordem democrática, não praticando atos que atentem contra ela. No terceiro capítulo, por meio de uma metodologia lógico-dedutiva das teorias sedimentadas, estuda-se o ato normativo que extinguiu a política de participação social em Conselhos Públicos e a Adin 6.121-DF, ajuizada contra o referido ato. O artigo identifica no acórdão matrizes jurídicas ainda em conflito no nosso ordenamento jurídico, em que pese a totalidade dos juízes do STF ressaltarem as virtudes da democracia participativa. Por fim, conclui-se que a extinção dos conselhos de forma irrestrita afronta o dever fundamental de não atentar contra o Estado Democrático de Direito, à luz da teoria discursiva da democracia de Habermas.
Single use negative pressure wound therapy in vascular patients: clinical and economic outcomes
Authors: Wikkeling Marald , Mans Judith , Styche Timothy 
Published: September 2021

Objective: Surgical site complications (SSCs) such as dehiscence and infection are a common issue within vascular surgeries and are a key concern to payers and clinicians. It is estimated that occurrence of complications in vascular surgery can increment length of stay by 9.72 days and costs by £3776 per episode. The objective of this research was to determine the clinical and economic impact of the prophylactic use of single use negative pressure wound therapy (sNWPT) in postoperative femoral endarterectomy incisions within a single centre in the Netherlands. Method: Data were extracted retrospectively from the medical notes of patients consecutively treated for femoral endarterectomy between January 2013 and December 2019 in a single centre in the Netherlands. Since August 2016, patients were treated with the sNPWT device and their data were compared with that of patients treated before the introduction of the device. Data were extracted on SSCs and associated healthcare resource use, with comparisons made between the two patient groups. Results: The study included a cohort of 108 patients. Data of patients treated by standard care (n=64) showed 32 (50%) patients developed complications. This reduced significantly in patients treated with the sNPWT device (n=44) of whom eight (18.2%) developed a postoperative complication (p=0.0011). Average postoperative costs per patient were €3119 for those in the standard care group and €2630 where the sNPWT device was used. Conclusion: sNPWT provided clinical and economic benefits over standard care in the treatment of femoral endarterectomy patients, significantly reducing rate of complication and their associated costs.
Community setting survey evaluating AQUACEL dressings
Authors: Carrere Caroline , Nghi Jérôme , Duchier Amandine , Hachette-Gustin Hélène , Vasseur Nathalie , Charvet Vincent , Tahar-Chaouch Fouzia , Zaoui Amaria , Deltour Nicolas , Yvon Claude 
Published: September 2021

Objective: This study aimed to collect and analyse real-life data to characterise the initial use of Hydrofiber Technology dressings for the management of exuding wounds in France. Method: An online survey of nurses provided data from patients managed with two dressings—AQUACEL Extra or AQUACEL Ribbon—as the primary dressing. At baseline, sociodemographic data, relevant medical histories and wound characteristics were recorded. The status of the wounds was then examined on days seven and 14 of management, together with scores of both clinician and patient satisfaction. Results: The survey included 1093 patients with a mean age of 65.9 years, comprising 53.3% women; 615 (56.3%) patients presented with acute wounds and 478 with hard-to-heal wounds. Wounds were reported to have healed or improved in 79.4% and 88.1% of the patients after 7 and 14 days, respectively. After 14 days, the wounds were smaller (p<0.001), and the percentage of sloughy wound bed tissue had decreased (p<0.001), while the percentage of granulation tissue and epithelialisation increased significantly (p=0.024 and p=0.047, respectively). Tolerance of the dressing was good, with low levels of pain reported, both while wearing the dressing and on removal. On day 14, nurses reported a high level of satisfaction, while 70% and 42.7% of patients with acute and hard-to-heal wounds, respectively, were ‘very satisfied’. Conclusion: The Hydrofiber Technology dressings aided wound healing when used in the management of a wide range of acute and hard-to-heal wounds in medical and surgical indications. User satisfaction was high from both healthcare professionals and patients.
Looking for more reliable biomarkers in breast cancer: Comparison between routine methods and RT-qPCR
Authors: Caselli Emanuele , Pelliccia Cristina , Teti Valeria , Bellezza Guido , Mandarano Martina , Ferri Ivana , Hartmann Kerstin , Laible Mark , Sahin Ugur , Varga Zsuzsanna , Lupi Chiara , Stracci Fabrizio , Sidoni Angelo 
Published: September 2021

Purpose
Decades of quality control efforts have raised the standards of immunohistochemistry (IHC), the principle method used for biomarker testing in breast cancer; however, computational pathology and reverse transcription quantitative PCR (RT-qPCR) may also hold promise for additional substantial improvements.

Methods
Herein, we investigated discrepancies in the assessment of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and marker of proliferation Ki67 comparing routinely obtained IHC (and FISH) data (ORI) with the results of manual (REV) and semi-automated (DIA) re-evaluation of the original IHC slides and then with RNA expression data from the same tissue block using the MammaTyper® (MT) gene expression assay.

Results
Correlation for ER and PR was high between ORI IHC and the other three study methods (REV, DIA and RT-qPCR). For HER2, 10 out of 96 discrepant cases can be detected between ORI and REV that involved at least one call in the equivocal category (except for one case). For Ki67, 22 (29.1%) cases were categorized differently by either REV alone (n = 17), DIA alone (n = 15) or both (n = 10) and 28 cases (29.2%) for RT-qPCR. Most of the discrepant Ki67 cases changed from low to high between the original and following assessment and belonged to the intermediate Ki67 expression range (between 9 and 30%).

Conclusions
Determination of the breast cancer biomarkers ER, PR, HER2 and Ki67 at the mRNA level shows high degree of correlation with IHC and compares well with correlations between original with subsequent independent manual or semi-automated IHC assessments. The use of methods with wider dynamic range and higher reproducibility such as RT-qPCR may offer more precise assessment of endocrine responsiveness, improve Ki67 standardization and help resolve HER2 cases that remain equivocal or ambiguous by IHC/FISH. In summary, our findings seem to configure RT-qPCR as a complementary method to be used in cases of either equivocal results or presenting, at the traditional determination assays, biomarkers expressions close to the cut-off values.
Writing for the Screen: Creative and Critical Approaches, 2nd ed., Craig Batty and Zara Waldeback (2019)
Authors: Williford Andrew Steven 
Published: September 2021

Review of: Writing for the Screen: Creative and Critical Approaches, 2nd ed., Craig Batty and Zara Waldeback (2019)
London: Red Globe Press, 310pp.,
ISBN: 9781352006087 (pbk), $27.99,
ISBN: 9781352006025 (hbk), $84.99
American Eccentric Cinema, Kim Wilkins (2019)
Authors: Lawler A. G. 
Published: September 2021

Review of: American Eccentric Cinema, Kim Wilkins (2019)
New York: Bloomsbury Academic, 224pp.,
ISBN: 9781501336928 (ebk), $35.95,
ISBN: 9781501336935 (epdf), $35.95,
ISBN: 9781501368110 (pbk), $39.95,
ISBN: 9781501336911 (hbk), $120.00
Phase relationships in the Fe-rich region of the Ce–Nd–B–Fe quaternary system at 773 K
Authors: Luo Ketong , Liang Jianlie , Zhu Jinming , Cui Xuehong 
Published: September 2021

Abstract
The Fe-rich corner of the Ce–Nd–B–Fe quaternary system at 773 K has been experimentally investigated by means of X-ray powder diffraction and scanning electron microscopy equipped with energy dispersive X-ray spectroscopy techniques. No quaternary compound was observed in this system. Ce2Fe14B and Nd2Fe14B were found to form the continuous solid solution (Ce,Nd)2Fe14B. Ce-Fe4B4 and NdFe4B4 also form the solid solution (Ce,Nd)-Fe4B4. The isothermal section consists of 8 three-phase regions and 2 four-phase regions.
La localisation de témoins oïliques médiévaux. Le DRFM comme levier méthodologique
Authors: Robecchi Marco 
Published: September 2021

Abstract
The recent Dictionnaire des régionalismes du français médiéval de l’Est (DRFM) constitutes an analysis of 389 lexemes contained in the corpus Documents linguistiques galloromans (DocLing, including about 2,350 documentary texts). These words represent concepts linked mainly to administration, commerce, and agriculture in the Eastern part of the Galloromance area (Champagne, Lorraine, Bourgogne, and Franche-Comté). More than half of these words are also attested in over 500 non-documentary texts of religious, literary, and practical nature. Such texts are notoriously difficult to localize, however, the regional words they contain aid this process considerably. In the present article, we distinguish three types of lexical regionalisms: « formal », « semantic », and « integral ». In the first section, we clarify the theoretical and phenomenological aspects of this distinction. We discuss the various roles of lexical regionalisms in the localization of non-documentary texts and, more specifically, the relative usefulness of formal regionalisms in this process. In the following sections, we demonstrate two methodological uses of regionalisms in non-documentary texts: 1. the localization of medieval texts or manuscripts (we provide 24 examples) and 2. the clarification or the resolution of ecdotical problems during the preparation of critical editions.
Die -ra/-se-Alternation beimsubjuntivo imperfectoim europäischen Spanisch: Eine soziolinguistische Korpusstudie
Authors: Feldhausen Ingo , Christ Christina 
Published: September 2021

AbstractWith the-ra/-se-alternation of thesubjuntivo imperfecto, the present study takes up a classic topic of Hispanic studies. The focus here, however, lies in the little-researched regional variation of this alternation in Spain, combined with an investigation of the sociolinguistic variables of gender, age and level of education, and their role in that alternation. For this purpose, we analyzed interview data from four cities (Madrid, Málaga, Santiago de Compostela, Valencia) taken from theCorpus del Proyecto para el estudio sociolingüístico del español de España y de América(PRESEEA). The results show significant differences between the locations with Madrid showing the highest use of-se-forms and a large variation between the interviewees, while in Santiago de Compostela the-ra-form predominates; intermediate values are attested for the rest. Regarding the sociolinguistic factors, a higher use of the generally rarer-se-form occurs among men, in the youngest age group and in the middle educational level. The study thus not only shows clear diatopic differences in the-ra/-se-alternation within Spain, but also provides indications of a trend towards a more frequent use of the-se-form among the younger generation.
Les anthroponymes italiens délexicaux formés sur des issues de protoroman régional */karne-lakˈsare/ ‘mardi gras’ ou : comment PatRom complète le LEI
Authors: Chambon Jean-Pierre , Germain Jean 
Published: September 2021

Abstract
The comparison of the lexical data gathered by the LEI and the medieval and contemporary anthroponymic data gathered by PatRom makes it possible to measure, on a concrete example (the delexical personal names formed on continuators of regional Proto-Romance */karne-lakˈsare/ ‘Shrove Tuesday’), the contributions, modest but not negligible, of anthroponymy to historical lexicology.
Reduced Dopamine Signaling Impacts Pyramidal Neuron Excitability in Mouse Motor Cortex
Authors: Swanson Olivia K. , Semaan Rosa , Maffei Arianna 
Published: September 2021

AbstractDopaminergic modulation is essential for the control of voluntary movement; however, the role of dopamine in regulating the neural excitability of the primary motor cortex (M1) is not well understood. Here, we investigated two modes by which dopamine influences the input/output function of M1 neurons. To test the direct regulation of M1 neurons by dopamine, we performed whole-cell recordings of excitatory neurons and measured excitability before and after local, acute dopamine receptor blockade. We then determined whether chronic depletion of dopaminergic input to the entire motor circuit, via a mouse model of Parkinson’s disease, was sufficient to shift M1 neuron excitability. We show that D1 receptor (D1R) and D2R antagonism altered subthreshold and suprathreshold properties of M1 pyramidal neurons in a layer-specific fashion. The effects of D1R antagonism were primarily driven by changes to intrinsic properties, while the excitability shifts following D2R antagonism relied on synaptic transmission. In contrast, chronic depletion of dopamine to the motor circuit with 6-hydroxydopamine induced layer-specific synaptic transmission-dependent shifts in M1 neuron excitability that only partially overlapped with the effects of acute D1R antagonism. These results suggest that while acute and chronic changes in dopamine modulate the input/output function of M1 neurons, the mechanisms engaged are distinct depending on the duration and origin of the manipulation. Our study highlights the broad influence of dopamine on M1 excitability by demonstrating the consequences of local and global dopamine depletion on neuronal input/output function.