bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal genetics influences fetal neurodevelopment and postnatal autism spectrum disorder-like phenotype by modulating in-utero immunosuppression
Authors: Jaini Ritika , Wolf Matthew R. , Yu Qi , King Alexander T. , Frazier Thomas W. , Eng Charis 
Published: June 2021

AbstractGenetic studies in ASD have mostly focused on the proband, with no clear understanding of parental genetic contributions to fetal neurodevelopment. Among parental etiological factors, perinatal maternal inflammation secondary to autoimmunity, infections, and toxins is associated with ASD. However, the inherent impact of maternal genetics on in-utero inflammation and fetal neurodevelopment in the absence of strong external inflammatory exposures is not known. We used the PtenWT/m3m4 mouse model for ASD to demonstrate the impact of maternal genetics on the penetrance of ASD-like phenotypes in the offspring. PtenWT/m3m4 (Momm3m4) or PtenWT/WT (MomWT) females, their offspring, and placental interface were analyzed for inflammatory markers, gene expression, and cellular phenotypes at E17.5. Postnatal behavior was tested by comparing pups from Momm3m4 vs. MomWT. Mothers of the PtenWT/m3m4 genotype (Momm3m4) showed inadequate induction of IL-10 mediated immunosuppression during pregnancy. Low IL-10 in the mother was directly correlated with decreased complement expression in the fetal liver. Fetuses from Momm3m4 had increased breakdown of the blood–brain–barrier, neuronal loss, and lack of glial cell maturation during in-utero stages. This impact of maternal genotype translated to a postnatal increase in the risk of newborn mortality, visible macrocephaly and ASD-like repetitive and social behaviors. Depending on maternal genotype, non-predisposed (wildtype) offspring showed ASD-like phenotypes, and phenotypic penetrance was decreased in predisposed pups from MomWT. Our study introduces the concept that maternal genetics alone, without any added external inflammatory insults, can modulate fetal neurodevelopment and ASD-related phenotypes in the offspring via alteration of IL-10 mediated materno-fetal immunosuppression.
LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion
Authors: Li Junjun , Qian Yuan , Zhang Chao , Wang Wei , Qiao Yisheng , Song Hao , Li Liyan , Guo Jiazhi , Lu Di , Deng Xingli 
Published: June 2021

AbstractLong noncoding RNAs (lncRNAs) and their crosstalks with other RNAs have been revealed to be closely related to tumorigenesis and development, but their role in invasive pituitary adenoma (IPA) remains largely unclear. In our study, LINC00473 was identified as the most upregulated lncRNA in IPA by whole transcriptome RNA sequencing (RNA-Seq). Further, its related signaling pathway LINC00473/miR-502-3p/KMT5A was obtained by constructing a competing endogenous RNA (ceRNA) regulatory network. Their expression in IPA and non-invasive pituitary adenoma (NIPA) tissues was verified by qRT-PCR. Then the effects and mechanisms of LINC00473 and its ceRNA network on the proliferation of pituitary adenoma (PA) cells were confirmed by gene overexpression or silencing techniques combined with CCK-8 assay, EdU staining, flow cytometry assay, and double luciferase reporter gene assay in PA cell lines AtT-20 and GT1-1 in vitro and in a xenograft model in vivo. LINC00473 is overexpressed in IPA and can promote PA cells proliferation. Mechanistically, overexpression of LINC00473 restricts miR-502-3p through the ceRNA mechanism, upregulates KMT5A expression, and promotes the expression of cyclin D1 and CDK2, which is conducive to the cell cycle process, thereby promoting the proliferation of PA cells, involving IPA progression.
The RNA-binding protein HuR is a novel target of Pirh2 E3 ubiquitin ligase
Authors: Daks Alexandra , Petukhov Alexey , Fedorova Olga , Shuvalov Oleg , Kizenko Alena , Tananykina Elizaveta , Vasileva Elena , Semenov Oleg , Bottrill Andrew , Barlev Nickolai 
Published: June 2021

AbstractThe RING-finger protein Pirh2 is a p53 family-specific E3 ubiquitin ligase. Pirh2 also ubiquitinates several other important cellular factors and is involved in carcinogenesis. However, its functional role in other cellular processes is poorly understood. To address this question, we performed a proteomic search for novel interacting partners of Pirh2. Using the GST-pulldown approach combined with LC-MS/MS, we revealed 225 proteins that interacted with Pirh2. We found that, according to the GO description, a large group of Pirh2-associated proteins belonged to the RNA metabolism group. Importantly, one of the identified proteins from that group was an RNA-binding protein ELAVL1 (HuR), which is involved in the regulation of splicing and protein stability of several oncogenic proteins. We demonstrated that Pirh2 ubiquitinated the HuR protein facilitating its proteasome-mediated degradation in cells. Importantly, the Pirh2-mediated degradation of HuR occurred in response to heat shock, thereby affecting the survival rate of HeLa cells under elevated temperature. Functionally, Pirh2-mediated degradation of HuR augmented the level of c-Myc expression, whose RNA level is otherwise attenuated by HuR. Taken together, our data indicate that HuR is a new target of Pirh2 and this functional interaction contributes to the heat-shock response of cancer cells affecting their survival.
Thermally induced transformation of a Cu4I4-based cluster to a Cu2I2-based cluster under mild conditions
Authors: Jiang Cheng-gang , Yuan Yan-fang , Meng Xiao-yu , Zhou Ke-Ke , Zhuang Gui-lin , Li Bao , Zhang Tianle 
Published: June 2021

Two isomeric cuprous-iodide complexes from a tetranuclear step-cubane Cu4I4 cluster to two butterfly-like Cu2I2 units have been synthesized at different temperatures, followed by a clear color change from yellow-orange to deep red.
Optimization research on hybrid energy storage system of high‐speed railway
Authors: Qu Kai , Yuan Jiaxin 
Published: June 2021

AbstractThe regenerative braking energy generated during the braking of high‐speed trains affects the power quality of the power grid. Recovery of regenerative braking energy is problem that needs to be solved urgently. The regenerative braking energy of high‐speed railway features high power and high energy. It is difficult to recover it only by using high power density supercapacitors or high energy density batteries. In this paper, a hybrid energy storage system (HESS) composed of supercapacitors and lithium‐ion batteries and its optimal configuration method are proposed for the purpose of obtaining maximum economic benefits for railroad systems. Then the economic benefits when using the HESS and the single energy storage system are compared from the perspective of whether the regenerative braking energy is fully recycled or not. Finally, a simulation analysis with actual load of a high‐speed railway station is performed. The highest benefit is achieved when the regenerative braking energy is partially recovered by the HESS, which can save 3% of the total cost per day and pay back the cost in eight years.
Machine learning and whale optimization algorithm based design of energy management strategy for plug‐in hybrid electric vehicle
Authors: Hou Zhuoran , Guo Jianhua , Xing Jiaming , Guo Chong , Zhang Yuanjian 
Published: June 2021

AbstractIn this paper, a novel energy management strategy with the improved adaptability to various conditions for plug‐in hybrid electric vehicle (PHEV) is proposed. The control parameters, derived from the benchmark test, are optimized offline for different driving conditions. The optimized parameters are implemented according to different driving behaviours identified online. The offline and online cooperation improves performance of energy management strategy in different driving conditions. Three main efforts have been made: Firstly, the valuable features that describe different driving conditions are extracted by random forest (RF) and the features are used for determining driving condition categories, utilized for online driving condition identification by support vector machine (SVM). Secondly, the control thresholds in the developed control strategy are optimized by whale optimization algorithm (WOA) under different driving conditions. The optimal control thresholds for different driving conditions will be called online after certain traffic condition is categorized. At last, simulation‐based evaluation is performed, validating the enhanced performance of the proposed methods in energy‐saving in different driving conditions.
A gradient descend method‐based none‐line‐of‐sight‐induced code delay searching in global navigation satellite system
Authors: Liu Di , Jiang Changhui , Zhu Jianliang 
Published: June 2021

AbstractIn urban areas with narrow streets and tall buildings, none‐line‐of‐sight (NLOS) or multipath (MP) signals severely attenuate the positioning accuracy of the global navigation satellite system (GNSS). vector tracking loop (VT) has been employed in NLOS/MP signal detection and correction; its feasibility and superior performance have been assessed and demonstrated in previous research studies. In VT, the navigation solutions are fed back to calculate the signal tacking parameters and complete the carrier and code tracking. The NLOS/MP reception induces code delay to the code tracking. The generated local signal replicas are mixed with the received signal; the code and carrier discriminators’ outputs are employed as the measurements to estimate the navigation solutions in the VT navigation filter. In order to extract the code delay induced by the NLOS or MP, multiple correlators (MCs) are usually utilised to search for the code delay. However, more correlators will bring much heavier computation load and a real position is necessary for calculating the signal tracking parameters. The strap‐down inertial navigation system (SINS) can generate short‐term accurate navigation solutions. In this work, a gradient descend (GD) method is designed for extracting the NLOS/MP‐induced delay in the correlators’ measurements level. The VT/SINS ultra‐tightly coupled navigation system (VT‐UTC) is developed to obtain mutual aiding between these individual systems. In VT‐UTC, the corrected SINS navigation solutions are employed for calculating the signal tracking parameters, and reliable navigation solutions from the SINS can help to extract the code delays induced by the NLOS. Two field tests with global positioning system L1 signal are carried out for assessing the performance of the GD method in the VT‐UTC framework. Comparisons between the MC and GD method show that the correlators’ computation reduces a lot since much fewer correlators are calculated in the code delay searching. Also, reliable navigation solutions from the SINS could help to calculate the signal tracking parameter while in‐view GNSS satellites are limited.
Synthesis of α-Deuterioalcohols by Single-Electron Umpolung Reductive Deuteration of Carbonyls Using D2O as Deuterium Source
Authors: Hou Yuxia , Lai Zemin , Ning Lei , Li Yixuan , An Jie , Li Hengzhao , Li Ailing 
Published: June 2021

AbstractDeuterium incorporation can effectively stabilize the chiral centers of drug and agrochemical candidates that hampered by rapid in vivo racemization. In this work, the synthetically challenging chiral-center deuteration of alcohols has been achieved via a single-electron umpolung reductive-deuteration protocol using benign D2O as deuterium source and mild SmI2 as electron donor. The broad scope and excellent functional group tolerance of this method has been showcased by the synthesis of 43 respective α-deuterioalcohols in high yields and ≥98% deuterium incorporations. The potential application of this versatile method has been exemplified in the synthesis of 6 deuterated drug derivatives, 1 deuterated human hormone, and 3 deuterated natural products. This method using D2O is greener and more efficient compared to traditional pyrophoric-metal-deuteride-mediated reductive deuterations.
Aproceros leucopoda (elm zigzag sawfly).
Authors: Blake Max 
Published: June 2021

Abstract

Elm zigzag sawfly is considered a minor pest within its native range in East Asia, but since first arriving in Hungary and Poland in 2003 the sawfly has spread rapidly through Europe and is continuing to expand its range. Severe localized defoliation has been recorded by the species throughout Europe on elms in a variety of situations. Most elm species are utilised as host plants, which combined with it being both parthenogenic and multivoltine means populations can build up rapidly in suitable areas. No specific predators are known, and whilst parasitoids have been described on the species in Europe no studies have investigated their efficacy at controlling elm zigzag sawfly. This species was removed from the EPPO Alert List in 2015 following no international action on the species being requested by the EPPO member countries.
Clover yellow mosaic virus.
Authors: Hammond Rosemarie 
Published: June 2021

Abstract

Clover yellow mosaic virus is not recommended for regulation as a quarantine pest by EPPO and is not included in the EPPO Alert List. A comprehensive pest risk analysis was performed by the UK Food and Environment Agency (FERA): https://secure.fera.defra.gov.uk/phiw/riskRegister/downloadExternalPra.cfm?id=3804.
Novel Application of Nutritional Biomarkers From a Controlled Feeding Study and an Observational Study to Characterization of Dietary Patterns in Postmenopausal Women
Authors: Neuhouser Marian L , Pettinger Mary , Lampe Johanna W , Tinker Lesley F , George Stephanie M , Reedy Jill , Song Xiaoling , Thyagarajan Bharat , Beresford Shirley A , Prentice Ross L 
Published: June 2021

AbstractDietary guidance emphasizes healthy dietary patterns, but supporting evidence comes from self-reported dietary data, which are prone to measurement error. We explored whether nutritional biomarkers from the Women’s Health Initiative Nutrition and Physical Activity Assessment Study Feeding Study (NPAAS-FS) (n = 153; 2010–2014) and the Women’s Health Initiative Nutrition and Physical Activity Assessment Study Observational Study (NPAAS-OS) (n = 450; 2006–2009) could identify biomarker signatures of dietary patterns for development of corresponding regression calibration equations to help mitigate measurement error. Fasting blood samples were assayed for a specific panel of vitamins, carotenoids, and phospholipid fatty acids; 24-hour urine samples were assayed for nitrogen, sodium, and potassium levels. Intake records from the NPAAS-FS were used to calculate Healthy Eating Index 2010 (HEI-2010), Alternative Healthy Eating Index 2010 (AHEI-2010), alternative Mediterranean diet (aMED), and Dietary Approaches to Stop Hypertension (DASH) scores. Scores were regressed on blood and urine nutritional measures for discovery of dietary pattern biomarkers using a cross-validated model R2 ≥ 36% criterion (stage 1). Next, stepwise models (P ≤ 0.10 for entry/removal) using NPAAS-OS data were used to regress stage 1 dietary pattern biomarkers on NPAAS-OS self-reported dietary pattern scores using a food frequency questionnaire, a 4-day food record, and a 24-hour recall (stage 2). HEI-2010 and aMED analyses met the cross-validated R2 ≥ 36% criterion in stage 1, while AHEI-2010 and DASH analyses did not. The R2 values for HEI-2010 stage 2 calibration equations were as follows: food frequency questionnaire, 63.5%; 4-day food record, 83.1%; and 24-hour recall, 77.8%. Stage 2 aMED R2 values were 34.9%–46.8%. Dietary pattern biomarkers have potential for calibrating self-reports to enhance studies of diet-disease associations.
Invited Commentary: Childhood Adiposity and the Onset of Puberty—It Turns Out There Is More to Be Learned
Authors: Chavarro Jorge E 
Published: June 2021

Abstract
The literature on the relationship between adiposity and the onset of puberty is extensive, both in size and in the length of time this question has been alive in the biomedical literature. It is easy to wonder, then, whether there is anything new to be learned in this crowded field. In this issue of the Journal, Deardorff et al. (Am J Epidemiol. 2022;191(1):7–16) show that it is still possible to innovate in this area. The authors report on the relationship between body mass index at age 5 years and a variety of markers of the onset of puberty. Consistent with the current literature, they show no association between body mass index at age 5 and pubertal onset in boys. They also show an association between obesity at age 5 with earlier onset of secondary sexual characteristics in girls, making an important subject-matter contribution that addresses many of the concerns of the existing literature in assigning causality. At the same time, the authors make an important, although less evident contribution to the practice of epidemiology for studies where the assessment of pubertal status is of interest.
Unexpected acute pulmonary embolism in an old COVID-19 patient with warfarin overdose: a case report
Authors: Coutrot Maxime , Delrue Maxime , Joly Bérangère S , Siguret Virginie 
Published: June 2021

Abstract

Background
Severe acute respiratory syndrome coronavirus 2 disease is strongly associated with a high incidence of thrombotic events. Anticoagulation could be a cornerstone in successfully managing severe forms of coronavirus disease 2019 (COVID-19). However, optimal anticoagulant dosing in elderly patients is challenging because of high risk of both thrombosis and bleeding.

Case summary
We present here the case of an 89-year-old patient receiving warfarin for atrial fibrillation and valvular heart disease, admitted to the intensive care unit for respiratory failure due to COVID-19. The patient presented with a severe epistaxis associated with warfarin overdose [international normalized ratio (INR) &gt; 10]. After a successful initial reversal using vitamin K per os, INR values greatly fluctuated up to 10, requiring repeated administrations of vitamin K. Despite starting low-molecular-weight heparin therapy at therapeutic dose as soon as INR value was below 2.0, the patient further developed an acute bilateral and proximal pulmonary embolism concomitantly with a sharp D-dimer increase. The combination of azithromycin intake, a known inhibitor of CYP2C9, with the presence of CYP2C9*2 and −1639G&gt;A VKORC1, two variants associated with warfarin hypersensitivity, have likely contributed to explain the warfarin overdose and the difficulty to reverse warfarin effect in this patient.

Discussion
This case report illustrates the complexity of COVID-19 pathophysiology and its management for physicians, especially in patients receiving vitamin K antagonists (VKAs). Infection, concurrent medication use, and pharmacogenetic factors involved in VKA metabolism and pharmacodynamics may lead to a loss of control of anticoagulation. Pulmonary embolism should still be considered in COVID-19 patients even with effective or overdosed anticoagulant therapy.
Intimate partner violence: analysis of current screening practices in the primary care setting
Authors: Perone Hanna R , Dietz Noella A , Belkowitz Julia , Bland Sarah 
Published: June 2021

Abstract

Background
Intimate partner violence (IPV) is a largely undetected occurrence in the United States reported by 36–50% of women in their lifetime and associated with extensive physical and psychological implications. Currently, conflicting recommendations exist regarding screening practices with the United States Preventative Services Task Force (USPSTF) and the World Health Organization (WHO) advocating for and against universal screening, respectively. With research suggesting that women are seldom asked about IPV during clinic visits, further information is needed regarding current screening practices.

Objectives
To identify current IPV screening approaches in the primary care setting and factors that may impact screening completion.

Methods
We retrospectively examined patients presenting for annual examinations at four university-associated primary care clinics in southeast Florida (n = 400). Patient demographics, screener demographics, screening completion, and screening results were collected from the medical record. Results were compared to depression and anxiety screenings due to comparable prevalence and screening recommendations. Pearson chi square and Fisher exact tests were utilized to compare screening rates by demographic characteristics.

Results
IPV screening occurred at a much lower frequency (8.5%) compared to screenings for anxiety (37.3%) and depression (71.3%). Among documented IPV screenings, 64.7% of encounters resulted in patient refusal to be screened. Screening rates were found to be marginally impacted by patient ethnicity (P = 0.052).

Conclusions
Findings of both low screening rates and low screening success raise significant concerns for the shortcomings of advocating for universal IPV screening. Therefore, additional studies are necessary to identify covert barriers to screening completion before universal inquiry is advised.
Conditions for gatekeeping when GPs consider patient requests unreasonable: a focus group study
Authors: Breivold Jørgen , Rø Karin Isaksson , Hjörleifsson Stefán 
Published: June 2021

Abstract

Background
Requests from patients that are regarded by GPs as unreasonable are a source of conflict between GPs and patients. This makes gatekeeping challenging, as GPs negotiate a struggle between maintaining the doctor–patient relationship, protecting patients from the harms of medical overuse and acting as stewards of limited health care resources. More knowledge of how GPs can succeed in these difficult consultations is needed.

Objective
To explore Norwegian GPs’ perceptions of conditions that can promote their ability to act as gatekeepers when facing patient requests which they consider ‘unreasonable’.

Methods
A qualitative study based on three focus groups with Norwegian GPs conducted in 2019, exploring consultations in which the patient made a seemingly unreasonable request, but the GP was able to navigate the consultation in a clinically appropriate manner. Thematic cross-case analysis of verbatim transcripts from the focus groups was carried out using Systematic Text Condensation.

Results
The analysis revealed three major themes among the conditions that the GPs considered helpful when faced with an ‘unreasonable’ patient request: (i) professional communication skills; (ii) a long-term perspective; (iii) acknowledgement and support of GPs’ gatekeeping role among peers and from authorities.

Conclusion
Professional communication skills and relational continuity need to be prioritized for GPs to maintain their role as gatekeepers. However, support for the gatekeeping role within the profession as well as from society is also required.