bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ablation of Hypoxia-induced mitogenic factor promotes cardiac repair after myocardial infarction by downregulating matrix metalloproteinase-9 expression in macrophage
Authors: Feng Li , Wang Qing , Li Yanjiao , Cheng Wanwen , Liu Jie , Liu Wenjuan 
Published: September 2021

Abstract
Objectives

Matrix metalloproteinase-9 (MMP9) functions as a collagenase, it promotes the infarct expansion and cardiac rupture after myocardial infarction (MI). This study sought to identify the mechanism mediating MI-induced MMP9 expression, and explore the new therapeutic target improving healing process after MI.
Materials and Methods

HIMF expression is manipulated by genetic ablation of HIMF in mice (Himf−/−), and adenoviral overexpression of HIMF by recombinant adenovirus encoding a green fluorescent protein (GFP)-tagged mouse HIMF in cultured neonatal rat ventricular myocytes (NRVMs), cardiac fibroblasts (CFs) and the murine macrophage RAW264.7 cells. The mouse model of left descending coronary artery ligation was established to investigate the expression of MMP9 and HIMF.
Results

MMP9 was predominantly expressed in macrophages of MI hearts, and positively correlated to the expression of HIMF, a marker for anti-inflammatory macrophage polarization. HIMF deficiency (Himf−/−) results in significantly decreased MMP9 expression, increased collagen deposition, reduced infarction size and improved cardiac contraction. Mechanistic analysis reveals that HIMF increases MMP9 expression by activating STAT3 signaling in murine macrophage RAW264.7. This was verified by the inhibited STAT3 phosphorylation in the MI heart of Himf−/− mouse.
Conclusions

This study identified a novel HIMF-STAT3-MMP9 regulation module in macrophages that influences collagen deposition in infarct heart, providing a new sight for MI treatment.
From education to welfare – A historical-exploratory approach for examining the role of madrassas
Authors: Mumtaz zahid 
Published: September 2021

Abstract
Much is written on the history of madrassas and their contributions to scholarship. In addition, several scholars have done considerable research to identify the causes of madrassas' growth, determine the linkage of madrassa education with breeding extremist ideologies and suggested possible areas for reforms. This particular aspect has been the topic of scholarly debates and a policy problem faced by many governments and international agencies, i.e., how madrassas can be reformed. However, not much literature could be located that draws parallels and dissimilarities between the madrassas of the golden age and the current era. This is important because any reform or policy is unlikely to succeed without considering a problem's historical and contextual factors. Hence this study adopts a historical-exploratory approach to identify the similarities and differences between the madrassas of the golden age of Islam and present times and explore the causes of such differences by utlising the case study of madrassas in Pakistan. Addressing these issues will make the following contributions: First, identifying the similarities and dissimilarities between the madrassas of the golden age and modern times requires a detailed exploration of madrassas' current state and their role, of which little is known. Second, identifying the factors responsible for the differences in madrassas' role from the golden age to modern times will help understand the implications of such differences on madrassas and society. Lastly, such implications will answer the repeated calls in the literature of formulating policies for reforming the madrassas that have been a significant policy problem faced by many countries.
Changes in cortical auditory evoked potentials in response to auditory training in elderly hearing aid users
Authors: ALCÂNTARA YARA BAGALI , TOLEDO WILLIANS WALACE FANTE , DE LIMA KAROLINE RIBEIRO , CARNAÚBA ALINE TENÓRIO LINS , CHAGAS EDUARDO FEDERIGHI BAISI , FRIZZO ANA CLAUDIA FIGUEIREDO 
Published: September 2021

Abstract
Objective

To compare the cortical auditory evoked potential responses pre-and post-Auditory Musical Training associated with hearing aid adaptation in elderly people with presbycusis. Design: this is a prospective, randomized, single-blind study. Study Sample: eight presbiacusis elderly people between 65 and 80 years, new hearing aid users, divided into two groups participated in the study: Hearing Aid Group: use of hearing aid; and Auditory Training Group: use of hearing aid in addition to musical auditory training for 16 sessions. All participants were submitted to cortical auditory evoked potential tests with verbal stimulation in two different moments: Initial assessment, carried out before hearing aid adaptation and auditory training, and after three months, final assessment at the end of the auditory training sessions. All participants were adapted bilaterally with digital mini hearing aids. Results: There was a decrease in the P3a latency component for the Auditory Training Group when initial and final assessment were compared. Conclusion: There was a change in the CAEP in elderly people with presbycusis in response to the Musical Auditory Training associated with the use of hearing aids in elderly people with presbycusis.
Peptides derived from gp43, the most antigenic protein from Paracoccidioides brasiliensis, form amyloid fibrils in vitro: implications for vaccine development
Authors: Cardim-Pires Thyago R. , Sant’Anna Ricardo , Foguel Debora 
Published: September 2021

Abstract
Fungal infection is an important health problem in Latin America, and in Brazil in particular. Paracoccidioides (P. brasiliensis and P. lutzii) is responsible for paracoccidioidomycosis, a disease that affects mainly the lungs. The glycoprotein gp43 is involved in fungi adhesion to epithelial cells, which makes this protein an interesting target of study. A specific stretch of 15 amino acids that spans the region 181-195 (named P10) of gp43 is an important epitope of gp43 that is being envisioned as a vaccine candidate. Here we show that synthetic P10 forms typical amyloid aggregates in solution in very short times, a property that could hamper vaccine development. Seeds obtained by fragmentation of P10 fibrils were able to induce the aggregation of P4, but not P23, two other peptides derived from gp43. In silico analysis revealed several regions within the P10 sequence that are capable of forming amyloid with steric zipper architecture. Besides, in-silico proteolysis studies with gp43 revealed that aggregation-prone, P10-like peptides could be generated by several proteases, which suggests that P10 could be formed under physiological conditions. Considering our data in the context of a potential vaccine development, we redesigned the sequence of P10, maintaining the antigenic region (HTLAIR), but drastically reducing its aggregation propensity.
Efficacy and safety of Dabigatran vs. Rivaroxaban among Asians with non-valvular atrial fibrillation：Protocol for a systematic review and meta-analysis
Authors: Jia Li , Zhou Mingming , Sun Li , Yu Luhai , He Xiangyan 
Published: September 2021

Abstract
Atrial fibrillation(AF) increases the risk of ischemic stroke and systemic embolism in patients. Moreover, Asian patients with AF are more likely to have ischemic stroke than non-Asian patients. Oral anticoagulants could effectively prevent thrombotic events. Dabigatran and Rivaroxaban are two most commonly used novel oral anticoagulants (NOACs) in Asia, but those clinicial studies in relation with them are mostly in American and European countries. Therefore, whether there are differences between Dabigatran and Rivaroxaban among Asian patients with AF in terms of efficacy and safety is still unknown. This systematic review and meta-analysis will mainly assess clinical efficacy and safety of Dabigatran versus Rivaroxaban in Asian patients with AF by a pooled analysis. We will follow the PRISMA (preferred reporting items for systematic reviews and meta-analyses) and the reporting MOOSE (Meta-analyses of Observational Studies in Epidemiology) when performing this study. Then Cochrane Library,Web of Science, PubMed and China national knowledge infrastructure will be searched for eligible retrospective investigation that report the efficacy and safety outcomes of AF patients who utilised Dabigatran or Rivaroxaban for stroke prevention in Asian countries. The abovementioned database will be comprehensively searched from inception to September 30, 2019 to locate all potentially eligible studies. Outcome measures will include safety and efficacy indicators. Safety indicators include intracranial hemorrhage, major bleeding and gastrointestinal bleeding. Efficacy indicators include systemic embolism and stroke. New evidence for clinical profile of Dabigatran versus Rivaroxaban in AF patients will be provided for decision-making of Asian patients.PROSPERO registration number: CRD42020156197
Formative Evaluation Prior to Implementation of a Brief Treatment for Posttraumatic Stress Disorder in Primary Care
Authors: Valentine Sarah E. , Fuchs Cara , Sarkisova Natalya , Olesinski Elyse A. , Elwy A. Rani 
Published: September 2021

Abstract
Background
Successful implementation of evidence-based treatments for posttraumatic stress disorder (PTSD) in primary care may address treatment access and quality gaps by providing care in novel and less stigmatized settings. Yet, PTSD treatments are largely unavailable safety net primary care settings. We aimed to collect data on four potential influences on implementation, including the degree of less-than-best practices, determinants of the current practice, potential barriers and facilitators of implementation, and the feasibility of a proposed strategy for implementing a brief treatment for PTSD.
Methods
Our mixed-methods developmental formative evaluation (Stetler et al., 2006) was guided by the Consolidated Framework for Implementation Research (CFIR), including a) surveys assessing implementation climate and attitudes towards evidence-based treatments and behavioral health integration and b) semi-structured interviews to identify barriers and facilitators to implementation and need for intervention and system augmentation. Participants were hospital employee stakeholders (N = 22), including primary care physicians, integrated behavioral health clinicians, community wellness advocates, and clinic leadership. We examined frequency and descriptive data from surveys and conducted directed content analysis of interviews. We used a concurrent mixed-methods approach, integrating survey and interview data collected simultaneously using a joint display approach to inform implementation efforts. We utilized a primary care community advisory board (CAB) comprised of employee stakeholders to refine interview guides, and apply findings to the specification of a revised implementation plan.
Results
Stakeholders described strong attitudinal support, yet therapist time and capacity restraints are major PTSD treatment implementation barriers. Patient engagement barriers such as stigma, mistrust, and care preferences were also noted. Recommendations based on findings included tailoring the intervention to meet existing workflows, system alignment efforts focused on improving detection, referral, and care coordination processes, protecting clinician time for training and consultation, and embedding a researcher in the practice.
Conclusions
Our evaluation identified key factors to be considered when preparing for implementation of PTSD treatments in safety net integrated primary care settings. Our project also demonstrated that successful implementation of EBTs for PTSD in safety net hospitals necessitates strong stakeholder engagement to identify and mitigate barriers to implementation.
Malaria Care-Seeking and Treatment Ideation among Gold Miners in Guyana
Authors: Olapeju Bolanle , Adams Camille , Wilson Sean , Simpson Joann , Hunter Gabrielle , Davis TrishAnn , Mitchum Lindsey , Cox Horace , James Kashana , Orkis Jennifer , Storey Douglas 
Published: September 2021

Abstract
Background
Although miners are a priority population in malaria elimination in Guyana, scant literature exists on the drivers of malaria-related behaviour. This study explores the relationship between gold miners’ malaria-related ideation and the adoption of malaria care-seeking and treatment behaviours including prompt care-seeking, malaria testing, and self-medication.
Methods
Data are from a cross-sectional quantitative survey of 1685 adult miners between the ages of 18–59 years who live in mining camps in Regions 1, 7, and 8. The analysis focused on miners who reported an episode of fever in the past year (n = 745). Malaria care-seeking and treatment ideation was defined as a composite additive score consisting of the following variables: general malaria knowledge, perceived severity, perceived susceptibility, beliefs, perceived self-efficacy, perceived norms, interpersonal communication, and perceived response efficacy. Multivariable logistic regressions explored the relationship between ideation on care-seeking/treatment behaviours, controlling for confounding variables.
Results
Most miners with a recent episode of fever had perceived risk (92%), self-efficacy (67%), susceptibility (53%) and high malaria knowledge (53%). Overall, miners' care-seeking/treatment ideation score ranged from 0 to 8 with a mean of 4.1. Ideation scores were associated with higher odds of care-seeking for fever (aOR:1.19; 95% CI: 1.04–1.36), getting tested for malaria (aOR: 1.22; 95% CI: 1.07–1.38) and lower odds of self-medication (aOR: 0.87; 95% CI: 0.77–0.99).
Conclusions
A national community case management initiative is using study findings as part of its scale-up, using volunteers to make testing and treatment services more accessible to miners. This is complemented by a multi-channel mass media campaign to improve miners’ ideation. Communication messages focus on increasing miners’ knowledge of malaria transmission and symptoms, encourage positive beliefs about malaria testing and volunteer testers, promote evidence about the effectiveness of testing, and reminders of how quick and easy it is to get a malaria test with the community case management initiative. Study findings also have implications for efforts to eliminate malaria across the Guiana Shield.
Comprehensive genome-wide identification, characterization, and expression analysis of CCHC zinc finger gene family in wheat (Triticum aestivum L.)
Authors: Sun Aolong , Li Yongliang , Zou Xiaoxiao , Chen Fenglin , Cai Ruqiong , Xie Hai , Xiao Wenjun , Guo Xinhong 
Published: September 2021

Abstract
Background: The CCHC zinc finger proteins (CCHC-ZFPs) are transcription factors that play versatile roles in plant growth, development, and responses to biotic/abiotic stress. However, little is known about the CCHC-ZF genes in bread wheat (Triticum aestivum), an important food crop.
Results: In this study, 50 TaCCHC-ZF genes were identified and distributed unevenly on 21 wheat chromosomes. According to the phylogenetic features, the 50 TaCCHC-ZF genes were classified into eight groups with specific motifs and gene structures. 43 TaCCHC-ZF genes were identified as segmentally duplicated genes that formed 36 segmental duplication gene pairs. Additionally, the collinearity analyses between wheat and eight other representative plant species showed that wheat had closer phylogenetic relationships with monocots compared to dicots. A total of 636 cis-elements related to environmental stress and phytohormone responsiveness were identified in the promoter of TaCCHC-ZF genes. Moreover, GO enrichment results revealed that all 50 TaCCHC-ZF genes were annotated under metal ion binding and nucleic acid binding. 91 miRNA binding sites within the 34 TaCCHC-ZF genes were identified by miRNA targets analyses, indicating that the expression of TaCCHC-ZF genes could be regulated by the miRNAs. Based on published transcriptome data, 38 TaCCHC-ZF genes were identified as DEGs, and 15 TaCCHC-ZF genes among them were verified by qRT-PCR assays, which showed response to drought, heat, or simultaneous response of them.
Conclusions: This study systematically explored the gene structures, evolutionary characteristics, and potential roles during environmental responses of TaCCHC-ZF genes, providing a foundation for further investigation and application of TaCCHC-ZF genes in the molecular breeding of T. aestivum.
Keywords: Wheat, CCHC-ZFP genes, Structural analysis, Evolution, Abiotic stress, Expression patterns
Internal Friction of Angle Model of Particles
Authors: Zegzulka Jiri , Necas Jan , Rozbroj Jiri , Gelnar Daniel , Gomes Alvaro , Jezerska Lucie 
Published: September 2021

Abstract
Currently, pressure from industry to streamline processes by creating their simulation models, and thus to gradual digitization is increasing. The essence of representative simulation models of bulk materials is to understand the principles and laws of the real behavior of particles. The aim of this study is therefore to find and quantify the possibilities and principles of how particles can change their position relative to other particles. The possibilities of particle displacements were expressed using their specific trajectories and work ratios, or internal friction angle values. This created a new comprehensive model of the internal friction angle of particles independent of particle size. It enables the interpretation of the determined values of the angles of internal friction of particles and its application in the field of simulations of mass and process models. The model can be used to determine the basic composition of particles in volume and the dominant ways of their mutual displacements.
Evaluation of ATAD2 As a Potential Target in Hepatocellular Carcinoma
Authors: Ekin Umut , Yuzugullu Haluk , Ozen Cigdem , Korhan Peyda , Bagirsakci Ezgi , Yilmaz Funda , Yuzugullu Ozge Gursoy , Uzuner Hamdiye , Alotaibi Hani , Kirmizibayrak Petek Ballar , Atabey Nese , Karakülah Gökhan , Ozturk Mehmet 
Published: September 2021

Abstract
Purpose
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide with. lack of effective systemic chemotherapy. In this study, we aimed to evaluate the value of ATPase family AAA domain-containing protein 2 (ATAD2) as a biomarker and potential therapeutic target for HCC.
Methods
The expression of ATAD2 was tested in different HCC patient cohorts by immunohistochemistry and comparative transcriptional analysis. The co-expression of ATAD2 and proliferation markers was compared during liver regeneration and malignancy with different bioinformatics tools. The cellular effects of ATAD2 inactivation in liver malignancy was tested on cell cycle, apoptosis and colony formation ability as well as tumor formation using RNA interference. The genes affected by ATAD2 inactivation in three different HCC cell lines were identified by global gene expression profiling and bioinformatics tools.
Results
ATAD2 is overexpression is closely correlated with HCC tumor stage. There was gradual increase from dysplasia, well differentiated and poorly differentiated HCC, respectively. We also observed transient upregulation of ATAD2 expression during rat liver regeneration in parallel to changes in Ki-67 expression. ATAD2 knockdown resulted in apoptosis and decreased cell survival in vitro and decreased tumor formation in some HCC cell lines. However, three other HCC cell lines tested where not affected. Similarly, gene expression response to ATAD2 inactivation in different HCC cell lines was highly heterogeneous.
Conclusions
ATAD2 is a potential proliferation marker for liver regeneration and HCC. It may also serve as a therapeutic target despite heterogeneous response of malignant cells.
Combined effect of Ilizarov technique, low-intensity pulsed ultrasound, and teriparatide for limb reconstruction of Gustilo–Anderson type IIIc open tibial fracture with a large bone defect in elderly patients with severe osteoporosis: A case report
Authors: Nozaka Koji , Miyakoshi Naohisa , Mita Motoki , Shimada Yoichi 
Published: September 2021

Abstract
Background Gustilo–Anderson type IIIc tibial open fracture with large bone defects in severely osteoporotic elderly patients is a rare injury that may be a challenging clinical scenario.Case presentation This study presents the case of a 68-year-old man who sustained a Gustilo–Anderson type IIIc open tibial fracture with a large bone defect. The patient had severe osteoporosis and the bone was contaminated; therefore, we determined that the bone could not be returned to the tibia. The patient underwent acute limb shortening and gradual lengthening with an Ilizarov external fixator combined with low-intensity pulsed ultrasound and teriparatide administration for limb reconstruction, which allowed immediate full weight-bearing capacity. The fixator was removed at 12 months postoperatively, and by this time, the fracture had completely healed. At the most recent 5-year follow-up after the injury, the patient reported fully weight-bearing capacity without walking aids and had full knee and ankle range of motion.Conclusions To the best of our knowledge, this is the first study to report the use of combined Ilizarov technique, low-intensity pulsed ultrasound, and teriparatide for limb reconstruction of Gustilo–Anderson type IIIc open tibial fractures with large bone defects in elderly patients with severe osteoporosis.
Quantitative assessment of ecological operation effects based on flood pulses and ecology-economic coupling model: a case study of Three Gorges Reservoir, China
Authors: Li Jiqing , Huang Jing , Xue Zhiming , Liang Pengteng , Wu Yueqiu 
Published: September 2021

Abstract
Flood pulses are closely related to river ecosystem health. Reservoirs bring many benefits to flood control, power generation, shipping etc., but their attenuation effects on runoff flood pulses should not be ignored. Ecological operation can effectively reduce some negative ecological impacts brought by the reservoir. However, the inability to quantitatively assess ecological effects hinders the promotion of ecological operation in reservoir management. To solve this problem, we proposed 11 flood pulse indicators (FPI), a random simulation method and an ecology-economy coupling model in this study. In addition, we used four major Chinese carps as indicator species and the Three Gorges Reservoir as a case study to test the role of flood pulses in improving the ecological operation effects of the reservoir from the fish protection perspective. The results show that: (1) FPI can be controlled by the reservoir and reflect the flood pulse characteristics of runoff. (2) Random simulation method guides managers to optimize the discharge and formulate eco-friendly operation schemes. (3) Ecology-economy coupling model helps managers analyze the relationship between ecological operation effects and economic benefits. A comprehensive assessment can improve the acceptance of ecological operation, which is conducive to the sustainable development of river ecosystem.