bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of SGOL2 Predicts Poor Prognosis and Facilitates Hepatocellular Carcinoma Progression via Dysregulating the Cell Cycle by Directly Activating MAD2
Authors: Hu Qingqing , Hu Xiaochu , Zhao Yalei , Zhang Lingjian , Yang Ya , li lanjuan 
Published: June 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Shugoshin-like protein 2 (SGOL2) is a centromeric protein that ensures the correct and orderly process of mitosis by protecting and maintaining centripetal adhesions during meiosis and mitosis. However, the role of SGOL2 in cancer is not well understood. Methods: The mRNA and protein levels of SGOL2 and survival analysis were conducted in The Cancer Genome Atlas (TCGA) and further validated in 2 independent cohorts. Differential genes correlated with SGOL2 and mitotic arrest deficient 2 like 1 (MAD2) were obtained using LinkedOmics. Subsequently, loss-of-function and rescue assays were carried out in vitro and in vivo to assess the functions of SGOL2 in hepatic tumorigenisis. Findings: We found that SGOL2 was significantly overexpressed in HCC and predicted unfavorable overall survival in HCC patients. Next, we identified 47 differentially expressed genes positively correlated with both SGOL2 and MAD2 to be mainly involved in the cell cycle. In addition, SGOL2 downregulation suppressed the migration, invasion, proliferation, stemness and EMT of HCC cells and inhibited tumorigenesis in vivo. Furthermore, SGOL2 promoted tumor proliferation by activating MAD2-induced cell cycle dysregulation, which could be reversed by the MAD2 inhibitor M2I-1. We also proved that SGOL2 activated MAD2 by directly binding with MAD2. Conclusions: The results of this study showed that SGOL2 acts as an oncogene in HCC cells by directly activating MAD2 and then dysregulating the cell cycle, thereby providing a potential target for HCC patients in the future.
Decreasing Formaldehyde Emission from Particleboard Panels Fabricated by Adding Newly Phenolic Compounds as a Scavenger to Urea Formaldehyde Resin
Authors: kord behzad , Movahedi Farnaz , Adlnasab Laleh , Ayrilmis Nadir 
Published: June 2021

Abstract
This study focused on the influence of some novel scavengers on the physical, mechanical, and formaldehyde emission of particleboard produced with urea-formaldehyde resin. Three different scavengers, alizarin red sulfonate, alizarin yellow-GG, and chromotropic acid, were incorporated to the UF resin at 1, 3, 5, and 7% (by weight) loading levels based on the oven-dried weight of the resin. Other manufacturing parameters were kept constant in the producrion of particleboards. The results indicated that the formaldehyde emission of the particleboards significantly reduced with increasing amount of the scavengers. The lowest formaldehyde emission was found in the specimens containing alizarin red sulfonate (0.38 mg/l), followed by chromotropic acid (0.43 mg/l), and alizarin yellow-GG (0.49 mg/l), respectively, at 7 wt% loading level of the scavengers. Although the physical and mechanical properties of the particleboards decreased with the increasing content of the scavengers, they met the requirements of particleboards for interior fitments (including furniture) for use in dry conditions (P2 grade) of EN 312 standard. According to the results of technological properties and formaldehyde emission of the particleboards, it can be said that alizarin red sulfonate is the best scavenger among the investigated scavengers.
Neuropsychiatric Functioning in CDLS: A Detailed Phenotype and Genotype Correlation
Authors: Ajmone Paola Francesca , Allegri Beatrice , Cereda Anna , Michelini Giovanni , Dall'Ara Francesca , Mariani Milena , Rigamonti Claudia , Selicorni Angelo , Vizziello Paola , Costantino Maria Antonella 
Published: June 2021

Abstract
Background: Cornelia de Lange Syndrome (CdLS) is a rare genetic disorder and its phenotype clinical expression is widely variable. Despite the growing interest on genotype-phenotype correlations and on behaviour phenotype of genetic syndromes, specific studies in CdLS cohorts evaluating the correlations between genotype and neurodevelopmental characteristics, Behaviour and communicative aspects are limited, most of these studies are descriptive and there is a lack of specific assessment protocols.Methods: Neurodevelopmental and Behavioural phenotype of all the patients (N = 38) was assessed using a specific neuropsychiatric protocol, concerning Intellectual Quotient (IQ), General Quotient of Development (GQ), communicative skills, behavioural aspects and adaptive behaviour based on direct and indirect evaluation. Subsequently, we searched for possible genotype–phenotype correlations comparing individuals with NIPBL variants (CdLS NIPBL mutated group) and patients with negative molecular results (CdLS clinical diagnosis group). Results: The first part of the study showed a higher percentage of subjects with normal Intellectual Quotient (IQ) and Borderline Intellectual Functioning if compared with previous data; adaptive skills were lower than expected for age in all participants and the weakest areas were Socialization, Motor Skills, and Communication. Expressive language was more compromised than receptive language, nevertheless receptive abilities were also impaired. 39.5% of the sample presented with Autism Spectrum Disorder (ASD). By stratifying CdLS phenotypes by genetic, NIPBL mutated individuals demonstrated a worse trend in cognitive functioning, in communication expressive skills, in motor skills and in ASD symptoms in comparison with the Clinical Diagnosis Group. Individuals with non-Truncating mutation (mostly missense) displayed better abilities in Communication and in relational aspects with no ASD while Truncating individuals presented with worse abilities in Daily Living Skills, Socialization, Motor Skills, and Communicative abilities. Conclusions: These findings should increase our awareness of the strengths and weaknesses points in CdLS individuals to guide appropriate targeted management; interventions addressing communicative impairments represent a clinical priority in CdLS patients.
Categorization of Patients With Systemic Lupus Erythematosus Using Disease Activity, Patient-Reported Outcomes and Transcriptomic Signatures
Authors: Arcani Robin , Jouve Elisabeth , Chiche Laurent , Jourde-Chiche Noemie 
Published: June 2021

Abstract
Objective

Patients with systemic lupus erythematosus (SLE) display symptoms that are not always related to disease activity and may distort clinical trial results. Recently, a clinical categorization based on the presence of type 1 (inflammatory manifestations) and/or type 2 (widespread pain, fatigue, depression) symptoms has been proposed in SLE. Our aim was to develop a type 2 score derived from the Short-Form health survey (SF-36) to categorize SLE patients and to compare immunological and transcriptomic profiles between groups.
Methods

Seventeen items from the SF-36 were selected to build a type 2 score for 50 SLE patients (100 visits; LUPUCE cohort) and the SLEDAI was used to define type 1 symptoms. Patients were categorized in four groups: minimal (no symptoms), type 1, type 2 and mixed (both type 1 and type 2 symptoms). Clinical, immunological and transcriptomic profiles were compared between the groups.
Results

Type 2 scores ranged from 0 to 31, with a cut-off value of 14 (75th percentile). The sample categorization was: minimal in 39%, type 1 in 37%, type 2 in 9% and mixed in 15%. Type 2 patients were older than minimal patients and had a longer disease duration than type 1 and mixed patients. Immunological data and modular interferon signatures did not differ between the groups.
Conclusion

Patients with SLE can be categorized into four clinical groups using the SLEDAI score and our SF-36-derived type 2 score. This categorization is non-redundant with immunological or transcriptomic profiles and could prove useful to stratify patients in clinical trials.
Reference Gene Selection for RT-qPCR Assays in Different Tissues of Huperzia Serrata Based on Full-Length Transcriptome Sequencing
Authors: Fu Yanping , Niu Fei , Jia Hui , Wang Yanli , Feng Yana , Guo Bin , He Wei , Wei Yahui 
Published: June 2021

Abstract
Background

Huperzia serrata (H. serrata) produces various types of effectively lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer’s disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA, however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes.
Results

Based on the full-length transcriptome datasets and previous studies, thirteen candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by various analysis softwares. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA-biosynthetic related genes (lysine decarboxylas, RS-norcoclaurine 6-O-methyltransferase, cytochrome P45072A1 and copper amine oxidase). The data suggested that the expression trends of HupA-biosynthetic related genes were consistent with them in transcriptome sequencing in different tissue of H. serrata.
Conclusions

This study screened the best reference genes GAPDHB and HisH2A in different tissues of H. serrata, which provides suitable normalization for analyzing the expression of HupA-biosynthetic gene in transcriptional level in H. serrata.
Lung Ultrasonography Decreases Radiation Exposure in Newborns with Respiratory Distress
Authors: Tandircioglu Umit Ayse , Yigit Sule , Oguz Berna , Kayki Gozdem , Celik Hasan Tolga , Yurdakok Murat 
Published: June 2021

Abstract
Chest X-ray(CXR) is commonly used as a first line imaging method to diagnose the reason of respiratory distress in NICUs.Lung ultrasound is a new diagnostic tool for lung imaging. We aimed to determine the decrease in the number of CXRs on the first day of life in newborns with respiratory distress,with the use of lung ultrasonography. From January 2019 to June 2020,104 newborn infants hospitalized in the NICU with respiratory distress on the first day of life enrolled in this study(ClinicalTrials.govIdentifier NCT04722016).We used ultrasound as the first line technique for lung imaging.CXR was taken to determine endotracheal tube and umbilical catheter position or if considered necessary by the physician in charge of the infant.We calculated decreased number of CXR for every patient and evaluated the estimated decrease in radiation exposure. 104 neonates with median 36 weeks(25–40)gestational age and birth weight 2410gr(600–4100) enrolled in the study.Seventy(67,3%)of these babies were male.In the study group,24(23,1 %)patients were diagnosed with respiratory distress syndrome(RDS),49(47,1 %) patients with transient tachypnea of newborn(TTN),27(26 %) with pneumonia,4(3,8 %)with congenital heart diseases.Lung ultrasonography were performed 210 times for all infants,but CXRs were performed a total of only 107 times.CXR wasnot taken in 27 of the patients with a diagnosis of TTN,in 2 of the patients with a diagnosis of congenital pneumonia,and in one of the patients with congenital heart disease.The rate of patients who have never had a chest x-ray was 28,8%.Conclusions:We observed that usage of lung ultrasonography decreased the number of chest X-ray and radiation exposure in newborns with respiratory distress.
Effect of Amylolytic and Cellulolytic Enzymes on Whole Plant Corn Silage: Characteristics of Silage and Animal Digestion
Authors: GANDRA JEFFERSON RODRIGUES , Nunes Alanne T. , Oliveira Euclides R. , Silva Mávio S. J. , Pedrini Cibeli A. , Machado Fabio S. , Silva Geleice K. R. , Gandra Erika R. S. , Mendes Paulo V. C. , Pause Alzira G. S. 
Published: June 2021

Abstract
Enzyme additives have been added to forage at ensiling to improve nutritive value. The aim of this study was to evaluate effects of adding exogenous enzymes to silage on fermentative losses and profile, aerobic stability, chemical composition, in vitro degradation, microbial quality, and nutrients intake and digestibility. Treatments were control (CON); addition of exo-1,4-α-glycosidase glucoamylase (GLU; Kerazyme 3035, Kera Nutrição Animal, Bento Gonçalves, Brazil); addition of β-glucan 4-glucanhydrolase (CEL); and GLU + CEL. Data from the silo experiment were analyzed using PROC MIXED of SAS, with fixed effects of glucoamylase and cellulase, and interaction effect between them. In vivo experiment analyses also included fixed effect of Latin Square and period, and random effect of animal within Latin Square. CEL increased (P ≤ 0.038) gas losses and effluents production, CEL and GLU decreased (P = 0.039) DM recovery compared to control but not differ from GLU+CEL. CEL silage had higher (P ≤ 0.021) starch and crude protein and in vitro digestibility of DM and NDF (P ≤ 0.032), while GLU had higher (P = 0.001) ADF. CEL showed lower (P = 0.012) ethanol content and higher (P = 0.02) anaerobic bacteria counts, while GLU showed higher (P = 0.012) lactate concentration and lower (P ≤ 0.002) counts of bacteria and fungi. Lambs fed with CEL presented higher (P ≤ 0.012) digestibility coefficients for DM, OM, CP and NDF. Decrease on DM recovery indicates no improvements on the nutritive value of silage. On the other hand, cellulolytic enzyme positively affected animal digestion.
Primary Pericardial Angiosarcoma: A Case Report and Literature Review
Authors: sun hong , zhao min 
Published: June 2021

Abstract
Primary angiosarcoma is extremely rare malignant tumor that has no typical symptoms and progress rapidly with poor prognosis. It is mesenchymal in origin and observed most frequently in the right atrium, cases in the pericardium is much more rare. Only few can detected in the early-stage allowing complete radical resection with a mean survival of 3 months to 1 year. There is few pericardial angiosarcoma reported among these years. The present study reports a case of a 44-year-old woman with primary pericardial angiosarcoma, who underwent a wide range of imaging methods, including transthoracic echocardiography, contrast-enhanced computed tomography (CT) and positron emission tomography-magnetic resonance imaging (PET-MRI). The patient recovered well after operation in two years and died due to the recrudescence and pulmonary metastases in April, 2020. We report the case for its rarity and revealing the early detection of primary pericardial angiosarcoma with imaging examinations is critical for prognosis. Finally a literature review is done.
MiR-323a-3p Inhibits Tumor Growth and Gefitinib Resistance Acquisition by Targeting EGFR/ErbB3 in Colorectal Cancer
Authors: Zhang Yuanzhou , Liang Shunshun , Xiao Bowen , Hu Jingying , Pang Yechun , Liu Yuling , Yang Juan , Ao Junpin , Wei Lin , Luo Xiaoying 
Published: June 2021

Abstract
Background: EGFR-TKIs are prone to develop acquired drug resistance in colorectal cancer and are only applicable in Kras wild type colorectal cancer patients. This study aimed to determine the reasons for the poor treatment efficacy of TKIs in Kras mutant CRC and to improve the treatment effect. Method: The RTK Phosphorylation Membrane array was used to detect and screen changes in phosphorylated protein levels in KRAS mutant-resistant CRC cells. qRT-PCR, western blot and TCGA database were applied for reporting the expression of ERGR and ErbB3 in CRC. Luciferase reporter and western blot examined the network of miR-323a-3p. RTCA, colony formation, CCK-8, caspase-3/7 activity and Flow cytometry probed the impacts of miR-323a-3p on CRC cell growth.Results: We illustrated that ErbB3 and EGFR were activated in gefitinib-resistant Kras mutant colorectal cancer cell lines. ErbB3 is highly expressed in Kras mutant patient tissues, and patients with ErbB3high/EGFRhigh had a poorer prognosis. Mechanically, We found and verified that the tumor suppressor miR-323a-3p simultaneously directly targeted EGFR/ErbB3 and inhibited tumor cell growth by activating the apoptosis pathway. Further functionality studies identified miR-323a-3p synergized with gefitinib to inhibit tumor growth, and this synergy prevented the development of acquired resistance to gefitinib in CRC cell lines.Conclusion: Accordingly, these data indicate that Kras mutant CRC TKI resistance occurs due to the activation of EGFR and ErbB3. Thus, miR-323a-3p has the potential to treat Kras-mutated colorectal cancer by targeting ErbB3/EGFR.
Influence Of Geometric Attribute On Friction Coefficient In Sheet Metal Stamping
Authors: Zhao Dewang , Zhao Kunmin , Song Huaihao , Ren Daxin , Chang Ying 
Published: June 2021

Abstract
This paper presents a test device to explore the influence of geometric attribute of the contact surface on friction coefficient along with sliding speed and contact pressure. Friction tests were conducted on a third-generation high strength steel QP980. The friction coefficients for different surface curvatures, contact pressures and sliding speeds were calculated and the influences of these factors were analyzed. The formula for calculating the friction coefficients between curved contact surfaces was derived. The relationship between bending induced surface roughness increase and friction coefficient was established. An enhanced friction coefficient model with pressure, velocity and curvature dependence was proposed. The enhanced friction model was applied to simulate the stamping of an automotive part and a better correlation was achieved.
The Correlation Between IgM and IgG Antibodies With Blood Profile In Patients Infected With COVID 19
Authors: Alibolandi Zahra , Ostadian Amirreza , Sayyah Saeed , Kashani Hamed Haddad , Ehteram Hassan , Banafshe Hamid Reza , Hajijafari Mohammad , Sepehrnejad Mahdi , Kashani Narjes Riahi , Azadchehr Mohammd-Javad , Nikzad Hossein , Hosseini Elahe Seyed 
Published: June 2021

Abstract
Objectives: This study aimed to determine the levels of IgM and IgG antibody response to the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 in coronavirus disease 2019 (COVID-19) patients with different disease severity.Methods: IgM and IgG antibody levels were evaluated via enzyme-linked immunosorbent assay (ELISA). In total, 100 patients with confirmed SARS-CoV-2 infection were enrolled in this study and viral RNA was detected by using Real-time PCR technique. Clinical and laboratory data were collected and analyzed after hospital admission for COVID-19 and two months post-admission. Results: The level of anti-SARS-CoV-2 antibody IgG was significantly higher in the severe patients than those in moderate and mild groups, 2 months after admission. Also, level of IgG was positively associated with increased WBC, NUT and LYM counts in sever than mild or moderate groups after admission to hospital.Conclusion: Our findings suggested that patients with severe illness might experience longer virus exposure times and have a stronger antibody response against viral infection. Thus, they have longer time immunity compared with other groups.
Lifetime Attributable Risk Estimates of Secondary Cancer after External Radiotherapy among Colorectal Cancer Survivors in Saudi Arabia
Authors: Abolaban Fouad , Djouider Fathi 
Published: June 2021

Abstract
Radiation-induced second cancer is one of the crucial late side effects of radiotherapy treatment of first cancer. Although the second cancer induction mechanism is not well understood yet, many factors are related to its occurrences, such as age at exposure, dose to the organ and surrounding tissues, treatment modalities, and family history of cancer. This study aims to provide long-term estimates of second cancer incidence amongst colon cancer survivors in Saudi Arabia. The lifetime attributable risk (LAR) after radiation treatment of the colon cancer was determined, between the age at exposure and up to 95 years, in a single-institution cohort of male and female cancer survivors whose age at treatment was in the range 43 to 85 years. Risk estimates varied significantly with age at exposure, gender, and organ dose.
Rice (Oryza Sativa L.) Whole Grain Genome-Wide Association Study for Neutral Sugar Content
Authors: Panahabadi Rahele , McKee Lauren S. , Ahmadikhah Asadollah , Ingvarsson Pär K. , Farrokhi Naser 
Published: June 2021

Abstract
Background Cell wall matrix polysaccharides are structurally complex and diverse, but our knowledge about their synthesis is limited. The building blocks (monosaccharides) of these polysaccharides have critical role in defining the number and the ultrastructure (size) of rice grains, and therefore would have great influence on seed vigor, yield and quality. Genome-wide association study (GWAS) is a method of choice for fine mapping of QTLs involved in defining plant cell wall structure and modifications. The monosaccharides that contribute to major cell wall matrix polysaccharides in whole grains of 197 rice accessions were quantified using acid hydrolysis and high-performance anion-exchange chromatography with pulsed amperometric detection. A GWAS of calculated monosaccharide content in rice whole grain (RWG) was carried out using 33,812 single-nucleotide polymorphisms (SNPs) to identify corresponding markers in 200 kbp flanking regions.Results In total, 49 significant SNPs contained in 19 genomic regions (QTLs) on eight chromosomes of rice were determined to be associated with monosaccharides content. The candidate genes in QTL regions included the following: arabinose content was associated with α-N-arabinofuranosidase, pectinesterase inhibitor, a glycoside hydrolase (GH) from family 16 (GH16 enzyme); xylose content was associated with ethylene-responsive element-binding protein and a pyruvate kinase; mannose content was associated with a MYB family transcription factor and S-domain receptor-like protein kinase; galactose content was associated with a glycocyltransferase (GT) from family 8 (GT8 enzyme), GRAS family transcription factor, glutathione S-transferase, GH16 and GH17 enzymes; fucose content was associated with a GH16 enzyme, lysine-rich arabinogalactan protein 19 and a receptor protein kinase; and finally rhamnose content was associated with OsFBX41, pectinesterase, COBRA-like protein, and OsSAUR13 (Auxin-responsive SAUR).Conclusion The results of this study should improve our understanding of the genetic basis of the factors that might be involved in the biosynthesis and turnover of major matrix polysaccharides present in RWG. Several QTLs were identified on different chromosomes, all are reported for first. Further, our data provide insight that will be useful in the design of future breeding programs, allowing breeders to use available genetic resources more effectively in meeting global food demand and supply.