bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial and Temporal Variability of the Gulf Stream Near Cape Hatteras
Authors: Andres M. 
Published: August 2021

AbstractIn situ observations from a 19‐month deployment of current‐ and pressure‐sensor equipped inverted echo sounders (CPIESs) along and across the Gulf Stream near Cape Hatteras capture spatial and temporal variability where this western boundary current separates from the continental margin. Regional hydrographic casts and two temperature cross‐sections spanning the Gulf Stream southeast of Cape Hatteras are used with the CPIESs' records of acoustic travel time to infer changes in thermocline depthDTand Gulf Stream position. Wave‐like Gulf Stream meanders are observed where the Stream approaches the separation location with periods less than 15 days, wavelengths less than 500‐km, and phase speeds between 40 and 70 km d−1. Though meander amplitudes typically decrease by ∼30% on the final approach to Cape Hatteras, some signals are still coherent across the Gulf Stream separation location. Temporal variability in meander intensity may be related to the Loop Current ∼1,400 km upstream. Mesoscale variability is strongest downstream of the separation location where Gulf Stream position is no longer constrained by the steep continental slope. Low frequency transport changes in the Florida Straits are correlated with sea‐surface height gradients along the entire South Atlantic Bight (SAB) and withDTinferred at the CPIES sites. The correlations withDTare likely due to coherent transport anomalies in the Gulf Stream approaching the separation location, which then drive Gulf Stream position changes downstream of the separation location. The patterns of coherent transport anomalies may reflect large‐scale atmospheric forcing patterns or rapid equatorward propagation of barotropic signals along the SAB.
STAT3/LINC00671 axis regulates papillary thyroid tumor growth and metastasis via LDHA-mediated glycolysis
Authors: Huo Nan , Cong Rui , Sun Zhi-jia , Li Wen-chao , Zhu Xiang , Xue Chun-yuan , Chen Zhao , Ma Lu-yuan , Chu Zhong , Han Yu-chen , Kang Xiao-feng , Jia Song-hao , Du Nan , Kang Lei , Xu Xiao-jie 
Published: August 2021

AbstractLactate dehydrogenase A (LDHA), a critical component of the glycolytic pathway, relates to the development of various cancers, including thyroid cancer. However, the regulatory mechanism of LDHA inhibition and the physiological significance of the LDHA inhibitors in papillary thyroid cancer (PTC) are unknown. Long non-coding RNA (lncRNA) plays a vital role in tumor growth and progression. Here, we identified a novel lncRNA LINC00671 negatively correlated with LDHA, downregulating LDHA expression and predicting good clinical outcome in thyroid cancer. Moreover, hypoxia inhibits LINC00671 expression and activates LDHA expression largely through transcriptional factor STAT3. STAT3/LINC00671/LDHA axis regulates thyroid cancer glycolysis, growth, and lung metastasis both in vitro and in vivo. In thyroid cancer patients, LINC00671 expression is negatively correlated with LDHA and STAT3 expression. Our work established STAT3/LINC00671/LDHA as a critical axis to regulate PTC growth and progression. Inhibition of LDHA or STAT3 or supplement of LINC00671 could be potential therapeutic strategies in thyroid cancer.
Human iPS-derived pre-epicardial cells direct cardiomyocyte aggregation expansion and organization in vitro
Authors: Tan Jun Jie , Guyette Jacques P. , Miki Kenji , Xiao Ling , Kaur Gurbani , Wu Tong , Zhu Liye , Hansen Katrina J. , Ling King-Hwa , Milan David J. , Ott Harald C. 
Published: August 2021

AbstractEpicardial formation is necessary for normal myocardial morphogenesis. Here, we show that differentiating hiPSC-derived lateral plate mesoderm with BMP4, RA and VEGF (BVR) can generate a premature form of epicardial cells (termed pre-epicardial cells, PECs) expressing WT1, TBX18, SEMA3D, and SCX within 7 days. BVR stimulation after Wnt inhibition of LPM demonstrates co-differentiation and spatial organization of PECs and cardiomyocytes (CMs) in a single 2D culture. Co-culture consolidates CMs into dense aggregates, which then form a connected beating syncytium with enhanced contractility and calcium handling; while PECs become more mature with significant upregulation of UPK1B, ITGA4, and ALDH1A2 expressions. Our study also demonstrates that PECs secrete IGF2 and stimulate CM proliferation in co-culture. Three-dimensional PEC-CM spheroid co-cultures form outer smooth muscle cell layers on cardiac micro-tissues with organized internal luminal structures. These characteristics suggest PECs could play a key role in enhancing tissue organization within engineered cardiac constructs in vitro.
ZNF768 links oncogenic RAS to cellular senescence
Authors: Villot Romain , Poirier Audrey , Bakan Inan , Boulay Karine , Fernández Erlinda , Devillers Romain , Gama-Braga Luciano , Tribouillard Laura , Gagné Andréanne , Duchesne Éma , Caron Danielle , Bérubé Jean-Sébastien , Bérubé Jean-Christophe , Coulombe Yan , Orain Michèle , Gélinas Yves , Gobeil Stéphane , Bossé Yohan , Masson Jean-Yves , Elowe Sabine , Bilodeau Steve , Manem Venkata , Joubert Philippe , Mallette Frédérick A. , Laplante Mathieu 
Published: August 2021

AbstractRAS proteins are GTPases that lie upstream of a signaling network impacting cell fate determination. How cells integrate RAS activity to balance proliferation and cellular senescence is still incompletely characterized. Here, we identify ZNF768 as a phosphoprotein destabilized upon RAS activation. We report that ZNF768 depletion impairs proliferation and induces senescence by modulating the expression of key cell cycle effectors and established p53 targets. ZNF768 levels decrease in response to replicative-, stress- and oncogene-induced senescence. Interestingly, ZNF768 overexpression contributes to bypass RAS-induced senescence by repressing the p53 pathway. Furthermore, we show that ZNF768 interacts with and represses p53 phosphorylation and activity. Cancer genomics and immunohistochemical analyses reveal that ZNF768 is often amplified and/or overexpressed in tumors, suggesting that cells could use ZNF768 to bypass senescence, sustain proliferation and promote malignant transformation. Thus, we identify ZNF768 as a protein linking oncogenic signaling to the control of cell fate decision and proliferation.
Parallel adaptation in autopolyploid Arabidopsis arenosa is dominated by repeated recruitment of shared alleles
Authors: Konečná Veronika , Bray Sian , Vlček Jakub , Bohutínská Magdalena , Požárová Doubravka , Choudhury Rimjhim Roy , Bollmann-Giolai Anita , Flis Paulina , Salt David E. , Parisod Christian , Yant Levi , Kolář Filip 
Published: August 2021

AbstractRelative contributions of pre-existing vs de novo genomic variation to adaptation are poorly understood, especially in polyploid organisms. We assess this in high resolution using autotetraploidArabidopsis arenosa, which repeatedly adapted to toxic serpentine soils that exhibit skewed elemental profiles. Leveraging a fivefold replicated serpentine invasion, we assess selection on SNPs and structural variants (TEs) in 78 resequenced individuals and discover significant parallelism in candidate genes involved in ion homeostasis. We further model parallel selection and infer repeated sweeps on a shared pool of variants in nearly all these loci, supporting theoretical expectations. A single striking exception is represented byTWO PORE CHANNEL 1, which exhibits convergent evolution from independent de novo mutations at an identical, otherwise conserved site at the calcium channel selectivity gate. Taken together, this suggests that polyploid populations can rapidly adapt to environmental extremes, calling on both pre-existing variation and novel polymorphisms.
Light-fueled transient supramolecular assemblies in water as fluorescence modulators
Authors: Chen Xu-Man , Hou Xiao-Fang , Bisoyi Hari Krishna , Feng Wei-Jie , Cao Qin , Huang Shuai , Yang Hong , Chen Dongzhong , Li Quan 
Published: August 2021

AbstractDissipative self-assembly, which requires a continuous supply of fuel to maintain the assembled states far from equilibrium, is the foundation of biological systems. Among a variety of fuels, light, the original fuel of natural dissipative self-assembly, is fundamentally important but remains a challenge to introduce into artificial dissipative self-assemblies. Here, we report an artificial dissipative self-assembly system that is constructed from light-induced amphiphiles. Such dissipative supramolecular assembly is easily performed using protonated sulfonato-merocyanine and chitosan based molecular and macromolecular components in water. Light irradiation induces the assembly of supramolecular nanoparticles, which spontaneously disassemble in the dark due to thermal back relaxation of the molecular switch. Owing to the presence of light-induced amphiphiles and the thermal dissociation mechanism, the lifetimes of these transient supramolecular nanoparticles are highly sensitive to temperature and light power and range from several minutes to hours. By incorporating various fluorophores into transient supramolecular nanoparticles, the processes of aggregation-induced emission and aggregation-caused quenching, along with periodic variations in fluorescent color over time, have been demonstrated. Transient supramolecular assemblies, which act as fluorescence modulators, can also function in human hepatocellular cancer cells.
Protective humoral and cellular immune responses to SARS-CoV-2 persist up to 1 year after recovery
Authors: Feng Chengqian , Shi Jingrong , Fan Qinghong , Wang Yaping , Huang Huang , Chen Fengjuan , Tang Guofang , Li Youxia , Li Pingchao , Li Jiaojiao , Cui Jianping , Guo Liliangzi , Chen Sisi , Jiang Mengling , Feng Liqiang , Chen Ling , Lei Chunliang , Ke Changwen , Deng Xilong , Hu Fengyu , Tang Xiaoping , Li Feng 
Published: August 2021

AbstractSARS-CoV-2 vaccination has been launched worldwide to build effective population-level immunity to curb the spread of this virus. The effectiveness and duration of protective immunity is a critical factor for public health. Here, we report the kinetics of the SARS-CoV-2 specific immune response in 204 individuals up to 1-year after recovery from COVID-19. RBD-IgG and full-length spike-IgG concentrations and serum neutralizing capacity decreases during the first 6-months, but is maintained stably up to 1-year after hospital discharge. Even individuals who had generated high IgG levels during early convalescent stages had IgG levels that had decreased to a similar level one year later. Notably, the RBD-IgG level positively correlates with serum neutralizing capacity, suggesting the representative role of RBD-IgG in predicting serum protection. Moreover, viral-specific cellular immune protection, including spike and nucleoprotein specific, persisted between 6 months and 12 months. Altogether, our study supports the persistence of viral-specific protective immunity over 1 year.
Kinetic photovoltage along semiconductor-water interfaces
Authors: Li Jidong , Long Yuyang , Hu Zhili , Niu Jiyuan , Xu Tiezhu , Yu Maolin , Li Baowen , Li Xuemei , Zhou Jianxin , Liu Yanpeng , Wang Cheng , Shen Laifa , Guo Wanlin , Yin Jun 
Published: August 2021

AbstractExternal photo-stimuli on heterojunctions commonly induce an electric potential gradient across the interface therein, such as photovoltaic effect, giving rise to various present-day technical devices. In contrast, in-plane potential gradient along the interface has been rarely observed. Here we show that scanning a light beam can induce a persistent in-plane photoelectric voltage along, instead of across, silicon-water interfaces. It is attributed to the following movement of a charge packet in the vicinity of the silicon surface, whose formation is driven by the light-induced potential change across the capacitive interface and a high permittivity of water with large polarity. Other polar liquids and hydrogel on silicon also allow the generation of the in-plane photovoltage, which is, however, negligible for nonpolar liquids. Based on the finding, a portable silicon-hydrogel array has been constructed for detecting the shadow path of a moving Cubaris. Our study opens a window for silicon-based photoelectronics through introducing semiconductor-water interfaces.
Spin–orbit torque driven magnetization switching in W/CoFeB/MgO-based type-Y three terminal magnetic tunnel junctions
Authors: Isogami Shinji , Shiokawa Yohei , Tsumita Atsushi , Komura Eiji , Ishitani Yugo , Hamanaka Kosuke , Taniguchi Tomohiro , Mitani Seiji , Sasaki Tomoyuki , Hayashi Masamitsu 
Published: August 2021

AbstractWe have studied current induced magnetization switching in W/CoFeB/MgO based three terminal magnetic tunnel junctions. The switching driven by spin—orbit torque (SOT) is evaluated in the so-called type-Y structure, in which the magnetic easy-axis of the CoFeB layer lies in the film plane and is orthogonal to the current flow. The effective spin Hall angle estimated from the bias field dependence of critical current (Ic) is ~ 0.07. The field and current dependence of the switching probability are studied. The field and DC current induced switching can be described using a model based on thermally assisted magnetization switching. In contrast, the 50 ns long pulse current dependence of the switching probability shows significant deviation from the model, even if contribution from the field-like torque is included. The deviation is particularly evident when the threshold switching current is larger. These results show that conventional thermally assisted magnetization switching model cannot be used to describe SOT induced switching using short current pulses.
A meta-analysis on the prevalence and characteristics of severe malaria in patients with Plasmodium spp. and HIV co-infection
Authors: Mahittikorn Aongart , Kotepui Kwuntida Uthaisar , De Jesus Milanez Giovanni , Masangkay Frederick Ramirez , Kotepui Manas 
Published: August 2021

AbstractCo-infection with malaria and human immunodeficiency virus (HIV) increases the severity and mortality rates of both diseases. A better understanding of the effects of co-infections could help in the diagnosis, prompt treatment, prevention, and control of malarial parasites among HIV-infected patients. In this systematic review and meta-analysis, we estimated the prevalence and characteristics of severe malaria (SM) caused by co-infection with HIV. We included relevant studies that were conducted between the years 1991 and 2018 and reporting on SM. We pooled the prevalence of SM in patients with co-infection, pooled odds ratios of SM in patients with co-infection and Plasmodium mono-infection, and differences in laboratory parameters such as parasite density and leucocyte counts, between co-infected and Plasmodium mono-infected patients. The meta-analysis included 29 studies (1126 SM cases). The pooled prevalence of SM in co-infected patients using the data of 23 studies (SM = 795 cases, all co-infection cases = 2534 cases) was 43.0% (95% confidence interval [CI] 31.0–56.0%; I2, 98.0%). Overall, the odds of SM from 18 studies were pooled. The odds of SM were significantly higher in co-infected patients than in Plasmodium mono-infected patients (OR 2.41; 95% CI 1.43–4.08; I2 = 85%; P = 0.001) and also significantly higher in children (OR 9.69; 95% CI 5.14–18.3; I2, 0%; P < 0.0001; two studies) than in adults (OR 2.68; 95% CI 1.52–4.73; I2, 79.0%; P = 0.0007; 12 studies). Co-infected patients with SM had a higher parasite density than those with Plasmodium mono-infection when the data of seven studies were analysed (SMD, 1.25; 95% CI 0.14–2.36; I2, 98.0%; P = 0.03) and higher leukocyte counts when the data of four studies were analysed (MD, 1570 cells/µL; 95% CI 850–2300 cells/µL; I2, 21.0%; P < 0.0001). Thus, the prevalence of SM among patients co-infected with Plasmodium spp. and HIV is high. Because co-infections could lead to SM, patients with Plasmodium spp. and HIV co-infection should be identified and treated to reduce the prevalence of SM and the number of deaths.
Genetic diversity analysis of Korean peanut germplasm using 48 K SNPs ‘Axiom_Arachis’ Array and its application for cultivar differentiation
Authors: Nabi Rizwana Begum Syed , Cho Kwang-Soo , Tayade Rupesh , Oh Ki Won , Lee Myoung Hee , Kim Jung In , Kim Sungup , Pae Suk-Bok , Oh Eunyoung 
Published: August 2021

AbstractCultivated peanut (Arachis hypogaea) is one of the important legume oilseed crops. Cultivated peanut has a narrow genetic base. Therefore, it is necessary to widen its genetic base and diversity for additional use. The objective of the present study was to assess the genetic diversity and population structure of 96 peanut genotypes with 9478 high-resolution SNPs identified from a 48 K ‘Axiom_Arachis’ SNP array. Korean set genotypes were also compared with a mini-core of US genotypes. These sets of genotypes were used for genetic diversity analysis. Model-based structure analysis at K = 2 indicated the presence of two subpopulations in both sets of genotypes. Phylogenetic and PCA analysis clustered these genotypes into two major groups. However, clear genotype distribution was not observed for categories of subspecies, botanical variety, or origin. The analysis also revealed that current Korean genetic resources lacked variability compared to US mini-core genotypes. These results suggest that Korean genetic resources need to be expanded by creating new allele combinations and widening the genetic pool to offer new genetic variations for Korean peanut improvement programs. High-quality SNP data generated in this study could be used for identifying varietal contaminant, QTL, and genes associated with desirable traits by performing mapping, genome-wide association studies.
Role of anterior segment optical coherence tomography angiography in the assessment of acute chemical ocular injury: a pilot animal model study
Authors: Tey Kai Yuan , Gan Jinyuan , Foo Valencia , Tan Bingyao , Ke Meng Yuan , Schmetterer Leopold , Mehta Jodhbir S. , Ang Marcus 
Published: August 2021

AbstractTo examine the use of anterior segment-optical coherence tomography angiography (AS-OCTA) in the assessment of limbal ischemia in an animal model chemical ocular injury. We conducted a prospective study using an established chemical ocular injury model in 6 rabbits (12 eyes), dividing the cornea limbus into 4 quadrants. Chemical injury grade was induced based on extent of limbal injury (0 to 360 degrees) and all eyes underwent serial slit-lamp with AS-OCTA imaging up to one month. Main outcome measure was changes in AS-OCTA vessel density (VD) comparing injured and control cornea limbal quadrants within 24 h and at one month. AS-OCTA was able to detect differences in limbal VD reduction comparing injured (3.3 ± 2.4%) and control quadrants (7.6 ± 2.3%; p < 0.001) within 24 h of ocular chemical injury. We also observed that AS-OCTA VD reduction was highly correlated with the number of quadrants injured (r = − 0.89; p < 0.001; 95% CI − 5.65 to − 1.87). Corneal vascularization was detected by AS-OCTA in injured compared to control quadrants (10.1 ± 4.3% vs 7.0 ± 1.2%; p = 0.025) at 1 month. Our animal pilot study suggests that AS-OCTA was able to detect limbal vessel disruption from various severities of acute chemical insult, and in the future, could potentially serve as an adjunct in providing objective grading of acute ocular chemical injury once validated in a clinical trial.
Predicting corneal refractive power changes after orthokeratology
Authors: Kang Pauline , Maseedupally Vinod , Gifford Paul , Swarbrick Helen 
Published: August 2021

AbstractThis study aimed to characterise corneal refractive power (CRP) changes along the principal corneal meridians during orthokeratology (OK). Nineteen myopes (mean age 28 ± 7 years) were fitted with OK lenses in both eyes. Corneal topography was captured before and after 14 nights of OK lens wear. CRP was calculated for the central 8 mm cornea along the horizontal and vertical meridians. The central-paracentral (CPC) power ratio was calculated as the ratio between maximum central and paracentral CRP change from individual data. There was a significant reduction in CRP at all locations in the central 4 mm of the cornea (all p < 0.001) except at 2 mm on the superior cornea (p = 0.071). A significant increase in CRP was evident in the paracentral zone at 2.5, 3 and 3.5 mm on the nasal and superior cornea and at 3.5 and 4 mm on the temporal cornea (all p < 0.05). No significant change in CRP was measured in the inferior cornea except decreased CRP at 2.5 mm (p < 0.001). CPC power ratio in the nasal and temporal paracentral regions was 2.49 and 2.23, respectively, and 2.09 for both the inferior and superior paracentral corneal regions. Our results demonstrates that OK induced significant changes in CRP along the horizontal and vertical corneal meridians. If peripheral defocus changes are inferred from corneal topography, this study suggests that the amount of myopia experienced on the peripheral retina was greater than twice the amount of central corneal power reduction achieved after OK. However, this relationship may be dependent on lens design and vary with pupil size. CPC power ratios may provide an alternative method to estimate peripheral defocus experienced after OK.
Optimizing electrode structure of carbon nanotube gas sensors for sensitivity improvement based on electric field enhancement effect of fractal geometry
Authors: Yang Taicong 
Published: August 2021

AbstractWith the rapid development of carbon nanotubes gas sensor, the sensitivity of the sensing response is becoming more and more demanding. Different from the traditional studies on gas-sensitive materials, this paper combines the microscopic dimensional effects and physical properties of fractal geometry theory from the structure and morphology of sensor devices. The electrode structures of carbon nanotubes gas sensor is designed and optimized by Hilbert–Piano curve. Simulation experiments demonstrate that the electric field intensity and hot spot distribution of the fractal electrode are superior to those of the traditional interdigital electrode. Moreover, a novel chemiresistive gas sensor is fabricated combining the characteristics of carbon nanotubes and fractal geometry, and a test with exposure to nitric oxide showed that the sensors with fractal electrode structures improved the gas sensing sensitivity over sensors with traditional geometrical structures. It provides a new idea for the exploration of gas sensing technology.