bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of temperature patterns during storage of Scots pine and Norway spruce seeds on their germination and fungal infection rates
Authors: Nikolaeva M. A. , Varentsova E. Yu. , Safina G. F. 
Published: April 2021

Relevance of the study. One of the ways to maintain the genetic diversity of forest-forming species is to preserve highquality seed material ex situ. However, the relationship between the diversity of pathogenic mycobiota and the duration and methods of forest tree seed storage remains underexplored. The results of research into this problem can be used in forest seed production and forest phytopathology.Materials and methods. For our study we used seeds ofРinus sylvestris L. and Picea abies (L.) Karst. of the orthodox type, harvested in the period of 1996–2011 and stored under different temperatures: +20°С, +4°С, –18°С, and in liquid nitrogen vapor (–182°С) since 2011. Prior to their storage, seed samples were dried to a moisture content of 4.2–4.4% and hermetically packed. Seed germination was tested before and after three, five and eight years of storage, following GOST 13056.6-97 standards. The level of seed infection and the composition of pathogenic fungi were assessed.Results. After eight years of storage at +20°С, pine and spruce seed germination capacity decreased by 13–60%, depending on the year of harvesting. Seed storage at –18°С and –182°С allowed us to prevent seed infection and preserve seed viability. In most cases, the germination energy and germination capacity were negatively correlated with the level of seed infection. The diversity of pathogenic (mold) fungi on the surface of seeds was represented by ten genera; the most common were saprotrophs: Aspergillus P. Micheli, Penicillium Link, Rhizopus Ehrenb., Scopulariopsis Bainier.Conclusion. At the present stage of research, the success of seed storage at low and ultralow temperatures was demonstrated. We recommend cryopreservation for the long-term storage of improved and valuable seeds.
High resolution seismic refraction data interpretation: an example from Xiakou landslide, Sichuan, China
Authors: Pant Surendra Raj , Li T. , Wagner A. , Yi Fu Wei , Jiaman Cao 
Published: April 2021

Among several data processing and interpretation techniques available in seismic refraction, the Generalised Reciprocal Method (GRM) is now widely used. In the thick landslide zone of Xiakou, Sichuan, China, the depths to bedrock obtained from the conventional refraction interpretation by the Plus-Minus Method (Method t0) differed considerably from the results of electrical soundings and drilling. The conventional methods were not able to accommodate to the more likely geological situations (i.e. undetected layers and velocity inversion) in a thick landslide zone. Thicker the overburden more the variation occurred between the results of the GRM and conventional methods. The velocity of seismic waves in the refractor calculated by the Plus-Minus Method was also affected significantly from the bedrock topography. After applying the GRM, the calculated depths were found to be very near to those of the drilling and electrical sounding. The GRM also revealed that the changes in refractor velocity (recorded by conventional methods) were fictitious. The fictitious changes in refractor velocity were caused by the target refractor topography and surface topography.
In this paper two profiles are taken for discussion. The profile B-B' represents a case of thick overburden (more than 30 m) whereas the profile D-D' is taken from a thin overburden (less than 15 m) above the target refractor.
TOPICAL CHEMOTHERAPEUTIC ADJUNCTS IN NON-SURGICAL PERIODONTAL THERAPY – A RANDOMISED CONTROLLED DOUBLE BLINDED TRIAL.
Authors: Prasad Upendra , Deshpande Abhinav , Kamble Praneeta , Singh Abhishek , Apon Adiya , Prabhakar Geetika 
Published: April 2021

Introduction: Topical chemotherapeutic agents used in treatment of chronic periodontitis are antimicrobial agents which help in plaque control. The present study evaluates the efficacy of ornidazole gel with gold standard chlorhexidine gel when used as adjunct to scaling and root planing.
Material and Methods: 90 patients diagnosed as chronic periodontitis, having pocket depth ?3 mm and in good systemic health were selected by systematic sampling method and divided in 3 groups. In all 3 groups scaling and root planing was done. In group A, Ornidazole gel application was done while in Group B Chlorhexidine gel application was done while Group C was control group. Plaque Index (PI) and Gingival Index (GI) were recorded at baseline, 1 and 3 months while Probing pocket depth (PPD) and Clinical attachment level (CAL) were recorded at baseline and 3 months.
Results: PI and GI showed significant reduction in all three groups after 1 and 3 months compared to baseline. On inter group comparison, both PI and GI showed no significant difference in reduction of scores after 1 month and 3 months (p>0.05). PPD and CAL values showed statistically significant reduction after 3 months when compared to baseline values. The intergroup comparison revealed statistically significant reduction in group A and B compared to control group C (p<0.05).
Conclusion: Ornidazole showed comparable efficacy as Chlorhexidine when used as adjunct to scaling and root planing for treatment of chronic periodontitis.
Aprendizagem colaborativa como ferramenta no ensino de Anatomia Humana
Authors: Campos Sarah Lima , Borges Ana Kleiber Pessoa , Aversi-Ferreira Tales Alexandre , Silva Andressa Diúlia Dantas da , Campos Aline Lima , Abreu Tainá de , Silva Walesca Rodrigues 
Published: April 2021

Os conhecimentos anatômicos são imprescindíveis para o profissional da área de saúde, não obstante essa disciplina apresenta baixos desempenhos em relação ao processo de ensino e aprendizagem. Com isso esta pesquisa teve por objetivo propor o uso da aprendizagem colaborativa como ferramenta de auxílio ao aprendizado de anatomia. O método foi aplicado na forma de monitorias para os discentes dos cursos de Nutrição e Enfermagem da Universidade Federal do Tocantins (UFT), campus de Palmas-TO. Os discentes foram divididos em dois grupos, A e B, um grupo era responsável por abordar o conteúdo teórico e o outro pela explanação e condução do conteúdo prático, havendo rodízio destas atribuições. A eficácia do método foi avaliada por meio de questionário que empregou a escala de Likert e análise do rendimento dos discentes, através do diário de notas da disciplina. Para síntese dos resultados foi aplicado o Teste de Tukey. Observou-se que 95% dos participantes após a participação da metodologia afirmaram ter obtitido uma evolução positiva em relação ao aprendizado da anatomia humana e recomendaram a aplicação do método em outras disciplinas. Tendo o grupo de discentes participantes da metodologia colaborativa apresentado resultados superiores à média e ao grupo de discentes não participantes, com HSD (Diferença Honestamente Significativa) de 5%. Diante dos grandes desafios enfrentados no ensino de anatomia, o estímulo para execução da monitoria na forma colaborativa pode ser uma ferramenta para auxiliar nas estratégias de ensino.
Eventos adversos em pacientes cirúrgicos: Revisão integrativa
Authors: Santos Andressa dos , Pierobon Natielen , Zarichen Fernanda Aparecida , Wibbelt Gabriela Luísa , Bertoni Ana Paula Moro , Mota Camila do Carmo , Pulcini Luzia Souza Evangelista , Teixeira Silvelly Peres , Lenhani Bruna Eloise , Marcondes Larissa , Batista Josemar 
Published: April 2021

Objetivo: analisar as evidências científicas acerca dos eventos adversos ocorridos em pacientes cirúrgicos. Método: revisão integrativa de literatura, com coleta de dados de setembro a outubro de 2020, em três bases de dados: Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), Medical Literature Analysis and Retrieval System Online (MEDLINE) e Base de Dados em Enfermagem (BDENF). Foram selecionados artigos primários, publicados nos idiomas português, inglês e espanhol, sem recorte temporal. Resultados: foram elegíveis 18 artigos; 44,4% (n=8) foram conduzidos no Brasil. A amostra dos estudos variou de 19 a 11.949 registros/prontuários, com incidência/prevalência entre 3,5% a 21,8% e taxa de mortalidade estimada entre 1,82% a 18,4%. A prevenção dos eventos variou de 7,7% para casos relacionados à medicação a 90% para eventos gerais. Casos de eventos de origem infecciosa e de medicação/fluídos endovenosos foram prevalentes. Não seguir normas, falhas em habilidades técnicas, excesso de confiança e distrações foram fatores contribuintes para a ocorrência dos eventos adversos. Conclusão: a qualidade e a segurança do cuidado precisam ser aprimoradas em unidades de atendimento perioperatório, com destaque para os casos de infecção e de eventos adversos a medicamentos.
Percepção de tutores quanto à resposta clínica de cães braquicefálicos portadores de estenose de narinas submetidos a rinoplastia com o uso do laser de diodo
Authors: Mendes Junior Aguinaldo Francisco , Santos Gabriela Souza Leão Bezerra dos , Duarte Pâmela Cristina de Souza , Hotz Marina Ribeiro , Marques Juliana Letícia Rosseto , Almeida Vivian Gomes Ferreira de , Mothé Gabriele Barros , Soares Ana Maria Barros , Almosny Nádia  Regina Pereira 
Published: April 2021

Objetivando avaliar a resposta clínica de cães braquicefálicos portadores estenose de narinas submetidos a rinoplastia com o uso do laser de diodo, 62 tutores de cães braquicefálicos portadores de estenose de narinas foram entrevistados por meio de questionários aplicados presencialmente. Trinta e dois (51,6%) cães eram da raça Buldogue francês, 19 (30,6%) da raça Pug, 6 (9,7%) eram Buldogues ingleses e 5 (8,1%) Shih tzus. Quarenta cães (64,5%) eram machos e 22 (35,5%) fêmeas, com idade média de 29 meses. Ronco dormindo foi o sinal clínico mais relatado, 62/62 (100%), seguido de ronco acordado, 57/62 (92%), intolerância ao exercício, 50 (80%), apneia do sono, 30/62 (48%), e cianose 21/62 (34%). Espirro reverso, espirro e tosse foram citados por 49 (79%), 38 (61%) e 27 (43%) dos responsáveis, respectivamente. Foi constatado ainda que 82% (51/62) dos animais apresentavam flatulência, 80% (50/62) engasgo, 74% (46/62) vômito, 71% (44/62) regurgitação e 29% (18/62) disfagia. Uma redução acentuada dos sinais clínicos comuns à síndrome braquicefálica foi averiguada 30 dias após rinoplastia com laser de diodo, observando-se diminuição de 15% na ocorrência de ronco dormindo, 23% de ronco acordado, 22% de intolerância ao exercício, 30% de apneia, 48% de espirro reverso, 39% de espirro, 28% de tosse e 28% de cianose. Do mesmo modo, após a cirurgia, observou-se decréscimo de 14% na ocorrência de flatulência, 41% de engasgo, 45% de vômito, 39% nos casos de regurgitação e 19% de disfagia. O uso do laser de diodo mostrou-se eficaz na realização da rinoplastia em cães braquicefálicos portadores de estenose de narinas, promovendo redução dos sinais clínicos, com bons resultados estéticos e funcionais.
Effect of preoperative pneumococcal immunization on the incidence of respiratory infection in the postoperative period of cardiac surgery
Authors: Galli Stéfani , Costa Mário Augusto Cray da , Souza Ana Carolina Mello Fontoura de , Scomparin Dionizia Xavier 
Published: April 2021

Objective: To analyze whether the incidence of respiratory infections in the postoperative period of cardiac surgery is lower in patients immunized with the 13-valent pneumococcal conjugate vaccine. To study the main risk factors related to the development of infections in patients undergoing cardiac surgery. Methodology: 40 patients were included in the study, randomized 1: 1 to receive pneumococcal vaccine or placebo, 14 days before undergoing elective cardiac surgery. The incidence of infections in the 30 postoperative days was compared between groups. Results: There were 6 respiratory infections in the vaccine group and 2 in the placebo group (p=0.2024) and one death in the placebo group and none in the vaccine group (p=0.3173). Chronic obstructive pulmonary disease (p=0.003), diabetes mellitus (p=0.047) and peripheral arterial disease (p=0.0001) were listed as risk factors for the development of infections, which generated an increase in hospital stay (95% CI : 1.33- 7.13; p =0.0147) and ICU stay in the postoperative period (95% CI: 1.87- 33.45; p=0.0025). Conclusion: The application of pneumococcal vaccine 13v did not influence the development of postoperative pulmonary infections. Chronic obstructive pulmonary disease, diabetes mellitus and peripheral arterial disease were identified as risk factors for the development of infections, increasing the length of total hospital stay and time in the ICU.
Enteropatógenos associados a enterocolite em potros: Aspectos epidemiológicos, clínicos e métodos de diagnóstico
Authors: Souza Rafaela Pinto de , Mousquer Mariana Andrade , Müller Vitória , Curcio Bruna da Rosa , Nogueira Carlos Eduardo Wayne 
Published: April 2021

As enterocolites estão entre as afecções que mais acometem potros em sistemas de criações de equinos em todo o mundo. É estimado que até 80% dos potros nos primeiros seis meses de vida são acometidos por pelo menos um episódio de diarreia, em sua grande maioria episódios leves e transitórios, entretanto, os custos com o tratamento e a taxa de mortalidade que esta afecção pode gerar são impactantes para criadores e proprietários. O objetivo desta revisão é descrever os aspectos epidemiológicos, clínicos e métodos de diagnóstico dos principais enteropatógenos bacterianos, virais e parasitários envolvidos em episódios de diarreia em potros do nascimento aos 8 meses de vida. Foram utilizados artigos disponíveis nas plataformas Mendeley, MEDLINE, pubMed, SciELO. Entre os principais enteropatógenos causadores de diarreia em potros, podemos destacar as infecções causadas por Salmonella spp., Escherichia coli, Rhodococcus equi, Clostridium perfringens, Clostridium difficile, Lawsonia intracellularis, Rotavírus, Coronavírus e helmintos. A identificação de potros de risco e a implementação de protocolos terapêuticos com base nos exames complementares e identificação do agente etiológico são de grande importância para o sucesso no tratamento, controle e implementação de métodos de profilaxia nos sistemas de criação.
Secondary prevention of thrombosis with direct oral anticoagulants for hereditary hematogenous thrombophilia
Authors: Voytsekhovskiy V. V. 
Published: April 2021

Aim. The article describes the experience of using direct oral anticoagulants – drugs Dabigatran, Rivaroxaban and Apixaban for the secondary prevention of thrombosis in patients with the most common variants of hereditary hematogenous thrombophilia.Materials and methods. 86 patients were under observation: 53 men, 33 women. Only the registered fact of thrombosis, thromboembolism, ischemia or organ infarction was the basis for the diagnosis of hematogenous thrombophilia and further secondary prevention of thrombosis. In 80 cases, there was a combined form of thrombophilia. In addition to hereditary factors, there were acquired thrombogenic factors. Dabigatran etexilate (Pradaxa®) for the prevention of thrombus formation was prescribed to 41 patients aged 20 to 60 years; duration of admission from 12 months to 9 years, the dose of the drug was selected individually from 150 to 300 mg per day. Rivaroxaban (Xarelto®) for the prevention of thrombus formation was prescribed to 25 patients aged 18 to 54 years, duration of admission from 12 months to 7 years, the dose of the drug is 10-20 mg per day. Apixaban (Eliquis®) was prescribed to 10 patients, aged 30 to 50 years, the duration of admission was from 6 months up to 2 years, dosage 5-10 mg per day. For hyperhomocysteinemia, Angiovit® or Pentavit® was prescribed. Protein C and antithrombin III preparations with their congenital deficiency were used according to indications.Results. After Dabigatran was prescribed, only one patient had a recurrent pulmonary embolism due to low adherence to treatment. In other patients who were prescribed direct oral anticoagulants, no recurrence of thrombotic complications was recorded. No hemorrhagic complications were diagnosed with the use of Dabigatran and Apixaban. In 5 patients receiving Rivaroxaban, there were minor epistaxis; in three cases they stopped when the dose was reduced from 20 to 15-10 mg, two patients were transferred to dabigatran. No life-threatening bleeding has been reported.Conclusion. Dabigatran, Rivaroxaban and Apixaban are effective and safe drugs for antithrombotic therapy. The absence of the need for constant laboratory monitoring and extremely rare hemorrhagic complications makes it possible to use them in patients living in areas remote from large medical centers. Only 10 patients under our supervision with a diagnosis of “hematogenous thrombophilia” are currently taking warfarin. Timely diagnosis of the variant of hematogenous thrombophilia and the appointment of adequate anti-thrombotic therapy contributes to the relapse-free course of the disease.
Lung function in patients with lymphangioleiomyomatosis
Authors: Chernyak A. V. , Makarova M. A. , Avdeev S. N. 
Published: April 2021

Aim. To assess the functional parameters of the respiratory system and their impact on the survival of patients with lymphangioleiomyomatosis (LAM).Materials and methods. A study of lung function (spirometry, bodyplethysmography, diffusion test) was carried out in 131 patients with LAM (mean age 41±10 years), who were under observation and treatment in medical institutions in Moscow (Pulmonology Scientific Research Institute, Sechenov University, Moscow City Clinical Hospital named after D.D.Pletnev). The diagnosis of the disease was made based on the diagnostic criteria for LAM, formulated by a group of experts at the initiative of the European Respiratory Society in the guidelines for the diagnosis and treatment of LAM.Results. The mean VC value was 95±25% pred., FEV1 /VC − 56±20%, FEV1 − 64±31% pred., DLCO − 49±24% pred. DLCO, FEV1 /VC, FEV1 and VC were reduced in 87, 76, 70 and 27% of patients, respectively. The obstructive ventilatory defect was detected in the overwhelming majority of cases − in 75%, restrictive ventilatory defect − in 4 patients, mixed defect − in 1 patient. In 28 (21%) patients, there were no pulmonary ventilation disorders. The Cox regression model showed that baseline DLCO and FEV1 are the main factors determining the risk of death.Conclusion. In patients with LAM, the obstructive ventilatory defect predominates, the restrictive ventilatory defect is quite rare. The lung diffusion capacity is reduced in 87% of patients with LAM. Parameters of lung function are closely related to survival.
Epidemiological features of laryngeal cancer in the Far Eastern Federal District
Authors: Gordienko V. P. , Yanushevsky K. V. , Ekonia D. T. 
Published: April 2021

Aim. A scientific assessment of the main indicators of morbidity and mortality of the population from laryngeal cancer in the Far Eastern Federal District over the past ten years (2009-2019).Materials and methods. In the process of work, indicators of morbidity and mortality, reporting forms of statistical registration of oncological patients and regulatory documents of higher organizations, information on ten-year observations on the dynamics of changes in digital values of the investigated pathology were used.Results. The main data on morbidity and mortality of patients with laryngeal cancer are given in the Far Eastern Federal District, where 2019 new cases of malignant neoplasms were registered in 34152, which is 8.5% more than ten years ago. There were 2171 patients with laryngeal cancer under outpatient observation, i.e. 26.5% per 100.000 population in the region. In the structure of primary morbidity and total mortality among all departments of the respiratory system, laryngeal cancer ranked second after malignant neoplasms of the trachea, bronchi and lung. During the study period, the number of patients with advanced forms of the disease increased with a simultaneous decrease in the number of patients with stages I and II of the oncological process. Over the past ten years, the incidence of disease has increased more significantly in the female population. The percentage of actively identified patients during this time increased by several orders of magnitude, with a simultaneous increase in the index of the accumulation of populations of patients with laryngeal cancer. Morphological confirmation of the diagnosis was lower than the Russian average. The proportion of patients registered for five years or more did not reach the average republican values. Lethality in the first year since the diagnosis was established was characterized by dynamically decreasing digital values, despite the increase in the number of neglected cases, which can be explained by the high level of modern methods of treatment.Conclusion. Against the background of the increasing absolute number of patients with malignancies in the Far Eastern Federal District in 2019, there was a decrease in standardized indicators (world standard) of morbidity and mortality from laryngeal cancer in the male population, with an increase in the number of women who fell ill and died from this pathology.
The study of efficacy of soy-pumpkin products in the diet of healthy people for prevention of acute respiratory infections
Authors: Prikhodko A. G. , Statsenko E. S. , Borodin E. A. , Oshur L. Yu. , Kolosov A. V. , Odireev A. N. , Perelman J. M. 
Published: April 2021

Introduction. Hypoalimentation with the formation of protein-energy malnutrition can reduce the body’s resistance to acute respiratory infections (ARI). Soy and pumpkin can be a promising source of normalization of the diet.Aim. To study the effectiveness of the use of soybean-pumpkin products in the diet of healthy people to increase the resistance of the human body to ARI.Materials and methods. An open, randomized, parallel group study was conducted. Group 1 included 18 people who took a soy-pumpkin drink and dessert for 10 weeks in addition to their usual diet. Group 2 included 17 people on a regular diet. The concentration of vitamin E, cholesterol, α- and β-lipoproteins was determined in blood serum. Diene conjugates and unoxidized lipids were determined in the expired air condensate (EAC) by means of spectrophotometry. Lung function was assessed using spirometry.Results. According to the questionnaire survey, 50% of the participants in group 1 registered a positive dynamics from the regular use of soybean-pumpkin products. Only one participant underwent ARI. In the 2nd group, 7 people (41%) applied for medical help (χ2=4.43; p˂0.05), of which 6 (35%) had ARIs. At the end of the observation, an increase in vitamin E (from 34.1±0.68 to 36.4±0.55 μg/mL, p=0.0036), a decrease in total cholesterol (from 4.85±0.23 to 4.32±0.14 mmol/L, p=0.0013) and β-lipoproteins (from 2.92±0.24 to 2.31±0.18 mmol/L, p=0.0003). At the beginning of the study, in individuals of group 1, there was a relationship between age and the concentration of total cholesterol (r=0.50; p=0.036) and β-lipoproteins (r=0.47; p=0.048), as well as the weight and level of α-lipoproteins (r=-0.49; p=0.041), the concentration of unoxidized lipids (r=0.55; p=0.02), diene conjugates (r=0.48; p=0.043) in EAC. After regular use of soybean-pumpkin products, this correlation dependence was lost.Conclusion. Regular use of soy-pumpkin products for 10 weeks has a beneficial effect on the lipid profile of the blood, increases antioxidant defence, and reduces the susceptibility of a healthy person to ARI.