bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing perioperative outcomes in video-assisted thoracic surgery and robot-assisted thoracic surgery in lung cancer surgeries: a single-centre experience
Authors: Otorkpa Michael Joseph , Arif Salman , Gooseman Michael , Tentzeris Vasileios , Qadri Syed 
Published: February 2025

Abstract

Background
Lung cancer continues to be the primary cause of cancer-related deaths worldwide, with surgical resection recognised as the gold standard for non-small cell lung cancer. Recent innovations in thoracic surgery, including video-assisted thoracic surgery and robotic-assisted thoracic surgery, have revolutionised surgical methodologies. This study seeks to evaluate the perioperative outcomes of video-assisted thoracic surgery and robotic-assisted thoracic surgery in lung cancer procedures, concentrating on postoperative complications, length of hospital stay, and overall efficacy based on a single-centre experience.

Results
A total of 269 lung resections were conducted, with 176 (65.4%) completed using video-assisted thoracic surgery and 93 (34.6%) utilising robotic-assisted thoracic surgery. No notable disparities were observed in demographics or preoperative characteristics between the groups. The overall complication rates were 13.1% for video-assisted thoracic surgery and 16.1% for robotic-assisted thoracic surgery (p = 0.507). No substantial changes were observed in specific problems, such as air leaks and infections. The average postoperative duration was 3.26 days for video-assisted thoracic surgery and 3.70 days for robotic-assisted thoracic surgery (p = 0.252), with a 30-day mortality rate of 0.6% for video-assisted thoracic surgery and 0% for robotic-assisted thoracic surgery.

Conclusions
Video-assisted thoracic surgery and robotic-assisted thoracic surgery produce similar perioperative results in lung cancer procedures, showing no statistically significant differences in complications, length of hospital stay, or fatality rates. These findings correspond with the current literature, indicating that although robotic-assisted thoracic surgery may present specific benefits, the overall efficacy of both strategies is comparable. Future research using larger cohorts is crucial to gain a more in-depth understanding of these techniques and their long-term effects on patient outcomes.
Lentinula edodes mycelia extract abrogates chemotherapy-evoked cold and mechanical allodynia in mice
Authors: Tsubaki Masanobu , Kato Natsuki , Tateishi Keisuke , Yoshida Kengo , Matsuo Taira , Komori Rie , Morikawa Toshio , Nishida Shozo 
Published: February 2025

Abstract

Background
Chemotherapy-induced peripheral neuropathy (CIPN) is a detrimental outcome of various antineoplastic drugs, such as paclitaxel (PTX), vincristine (VCR), oxaliplatin (L-OHP), and bortezomib (BOR). CIPN results in pain and disability, thereby reducing quality of life and discontinuation of chemotherapy. Currently, the only effective treatment for CIPN is using duloxetine. Therefore, development of new treatments is necessary. Extract of Lentinula edodes mycelia (LEM) improves the quality of life for individuals undergoing chemotherapy treatment. As treatment with LEM may attenuate CIPN after chemotherapy, this study was conducted to determine whether treatment with LEM abrogates L-OHP-, PTX-, VCR-, and BOR-evoked cold and mechanical allodynia in mice.

Results
We found that LEM exhibits protective effects against cold and mechanical allodynia in mice treated with L-OHP, PTX, VCR, or BOR. We also found that the administration of L-OHP, PTX, VCR, and BOR elevated mRNA expression of Cav3.2, Cav3.3, and NR2A in the DRG of mice, whereas treatment with LEM abrogated L-OHP-, PTX-, VCR-, and BOR-induced Cav3.2 and NR2A mRNA expression. In addition, LEM treatment abrogated L-OHP-, PTX-, VCR-, and BOR-induced ERK1/2 phosphorylation in the DRG and spinal cord of mice. Furthermore, treatment with LEM reversed symptoms in mice that developed cold and mechanical allodynia after receiving L-OHP, PTX, VCR, or BOR.

Conclusion
These findings suggest that the attenuation of expression of phosphorylated ERK1/2, Cav3.2, and NR2A upon LEM treatment may be an effective prophylactic and therapeutic strategy against L-OHP-, PTX-, VCR-, and BOR-induced cold and mechanical allodynia.
Addressing Clinical Challenges in Aberrant Pharmacokinetics of Biologic Therapeutic Drugs: Investigating Sample Processing Procedure in the Immunoassays
Authors: Chen Yih-Wen , Davenport Olinda , Yu Nancy , Melendez Rachel , Nugteren James , Nijem Ihsan , Yan Weili , Hendricks Robert , Song Yuan 
Published: February 2025

Abstract
Bioanalytical Pharmacokinetics (PK) methods are designed for robust performance under rigorous regulatory compliance requirements to ensure the generated data is reliable and maintains integrity. In a phase 1 dose-finding clinical study, aberrant PK profiles of two co-administered biologics drugs were observed. Unexpectedly, we discovered high fill levels in collection tubes from the majority of samples. This led to the hypothesis that the highly filled tubes might cause difficulty in achieving complete sample thaw and thorough mixing at the time of sample analysis, potentially contributing to the abnormalities observed in the PK dataset. Evaluation of the impact of sample fill levels and processing procedure can be challenging since PK concentrations of study samples were unknown. Therefore, a systematic approach was employed to conduct a thorough examination using mock samples. The data illustrate a correlation between sample thawing and mixing process and the variability in the PK data. The concentrations from properly filled mock samples that underwent complete thawing and mixing showed 100% data reproducibility. In contrast, the concentrations from fully filled mock samples that did not follow the proper procedure showed sample recovery deviating by ± 30% from nominal value and exhibited considerable lack of precision. This data identified the root cause of aberrant PK, justifying revised sample preparation guidance and sample re-assay. Improved sample handling and subsequent reassay resolved the aberrant PK profile issues. In conclusion, this study reiterated that sample handling plays a crucial role in quality and reproducibility of PK data with immunoassays.

Graphical Abstract
Materials Compatibility Considerations for the Transition to Low Global Warming Potential Propellants for Pressurized Metered Dose Inhalers
Authors: Dohmeier Dan , Sen Atish , Cavecchi Alessandro , Matos João , Lostritto Richard , Nagao Lee 
Published: February 2025

Abstract
Pressurized metered dose inhalers (pMDI) are a vital therapy for the treatment of lung diseases such as asthma and chronic obstructive pulmonary disease (COPD). In pMDI, the propellants used to deliver the drug to the lungs are hydrofluorocarbons (HFC). However, the current HFCs in use have large global warming potential (GWP). In order to reduce or eliminate the use of propellants with large global warming potential, efforts are underway within the pharmaceutical industry to transition to the use of low GWP propellants in pMDI, while maintaining their effectiveness in treating disease. The current switch from higher GWP propellants mirrors the switch from chlorofluorocarbon (CFC) propellants to HFCs undertaken in the 1990’s, which was driven by ozone depletion concerns. In this paper, the International Pharmaceutical Aerosol Consortium on Regulation and Science (IPAC-RS) discusses aspects of the switch to low GWP propellants from the perspective of materials compatibility of pMDI components with low GWP propellants. Leveraging the learnings and advances in pMDI component materials implemented following the switch from CFCs, industry is well positioned to make the change. This paper describes the utility of the low GWP propellants being developed for use in pMDI and the learnings from the previous transition that are being leveraged by industry. The current state of development will be described, including a review of available literature supporting the transition to low GWP propellants.
Good Hair, Bad Math
Authors: Rand Erica 
Published: February 2025

Abstract
While most policing of gender categorization in sport concerns a human unit of one, I've been maneuvering, since 2019, within a human unit of two, as part of a white, queer, gender-nonconforming pairs team with my figure skating partner Anna Kellar. Situating our training and activism within the growing movement for change in the sport, this piece considers some of our experiences, the challenges, and deep pleasures as we work to advance the skills and rule changes required to test and compete; to navigate racialized gender binarism and cis-heteronormitivity sedimented in training, rule, and custom; and to develop performance vocabularies to legibly represent queer gender.
The Influence of Tumor Burden Score and Lymph Node Metastasis on the Survival Benefit of Adjuvant Chemotherapy in Intrahepatic Cholangiocarcinoma
Authors: Kawashima Jun , Endo Yutaka , Woldesenbet Selamawit , Khalil Mujtaba , Akabane Miho , Cauchy François , Shen Feng , Maithel Shishir , Popescu Irinel , Kitago Minoru , Weiss Matthew J. , Martel Guillaume , Pulitano Carlo , Aldrighetti Luca , Poultsides George , Ruzzente Andrea , Bauer Todd W. , Gleisner Ana , Marques Hugo , Groot Koerkamp Bas , Endo Itaru , Pawlik Timothy M. 
Published: February 2025

Abstract

Introduction
While postoperative adjuvant chemotherapy (AC) is generally recommended for intrahepatic cholangiocarcinoma (ICC), its benefit remains debated. This study aimed to identify patients that may benefit from AC following liver resection of ICC.

Methods
Patients who underwent liver resection for ICC between 2000 and 2023 were identified from an international multi-institutional database. Individual multivariable Cox models were used to evaluate the interaction between each prognostic factor and the effect of AC on survival.

Results
Among 1412 patients, 431 (30.5%) received AC. Both higher tumor burden score (TBS; hazard ratio [HR] 0.95, 95% confidence interval [CI] 0.91–1.00; p = 0.033) and metastatic lymph node status (HR 0.58, 95% CI 0.38–0.89; p = 0.014) demonstrated interactions with the survival benefit from receipt of AC. Interaction plots highlighted how AC was associated with improved survival beyond a TBS of approximately 6. Notably, among 555 (39.3%) patients with TBS <6 and N0 or Nx status, 5-year overall survival (OS) was no different between patients who received AC versus individuals who did not (55.1% [95% CI 48.9–62.1] vs. 58.7% [95% CI 49.8–69.2]; p = 0.900). In contrast, among 857 (60.7%) patients with TBS ≥6 or N1 status, AC was associated with improved 5-year OS (30.7% [95% CI 26.2–36.0] vs. 33.0% [95% CI 26.9–40.5]; p = 0.018).

Conclusions
TBS and lymph node status may be useful in a multidisciplinary setting to inform decisions about AC planning for ICC patients following curative-intent resection.
Ketamine in the Treatment of Depression
Authors: Kaczmarska Urszula Agnieszka , Cioch Michał Jakub , Nowak Julia , Woźniak Aleksandra , Hermanowicz Kamil , Najdek Agnieszka , Oleksy Daria , Komada Dawid , Doman Katarzyna , Mycyk Marcin 
Published: February 2025

Introduction
Depression is one of the most common mental illnesses. Over the course of a lifetime, several percent of the adult population suffers from depression. There are many treatments for depression like pharmacotherapy and psychotherapy. In this article, we wanted to focus on the effectiveness of ketamine in treating depression.
Aim of study
The study aimed to summarize the available knowledge on the use of ketamine in the treatment of depression. The epidemiology, etiology, side effects, and treatment methods, were summarized and described.
Materials and method
The literature available in the PubMed database was reviewed using the following keywords: “Ketamine”,” Depression”, and “Antidepressants”.
Conclusion
Without a doubt, ketamine has proven to be a new advocate for mental health research and therapeutics. After over half a century without novel targets for MDD (Major Depressive Disorder) treatment, the observation of ketamine’s rapid antidepressant effects in treatment-resistant depression has become a promising field that could represent a breakthrough in the understanding of MDD, and the possibility of reducing some of the major personal and global burden that depression is responsible for. It seems imminent then that a new era of different acting antidepressant strategies is upon us, and it is our responsibility to make a critical analysis of the potential benefits and harm inherent to novel therapeutics.
Impact of war stress on oral healt
Authors: Boitsaniuk Svitlana , Levkiv Mariana , Zaliznyak Marta , Manashchuk Nadiia , Patskan Liudmyla , Pohoretska Khrystyna , Chornii Nataliia 
Published: February 2025

Wartime greatly elevate the frequency, intensity, and severity of oral health issues among military personnel. This research outlines the complex effects of combat on oral health. It was revealed a high prevalence of periodontal problems and varying levels of dental caries in those affected. The conditions of war significantly impact the rise and seriousness of oral diseases among professional soldiers and those who are internally displaced.
Aim. The research aims to determine the relationship between stress and oral health in individuals exposed to chronic stress in wartime.
Materials and methods. The study was conducted on 68 professional soldiers in the Ukraine Army and 42 internally displaced persons. The control group consisted of 39 patients. The study was based on clinical examination and a questionnaire.
Results. Notable variations exist between the groups in wartime and peacetime based on how often they brush their teeth daily and their diet. A group of soldiers had significantly poorer oral health than civilians. According to the plaque index, аmong the military, only 7.1% have good oral hygiene (plaque index 0.0), 53.6% have fair oral hygiene (plaque index 0.1-1.9), and 39.3% have bad oral hygiene (plaque index 2.0-3.0).
Conclusion. Participation in war and time spent on battlefields have had a significant influence on the increased prevalence and severity of periodontal disease, decay, and diet.
Review of the Literature on the Impact of Teriparatide Use on Bone Healing
Authors: Bielecka Larysa 
Published: February 2025

Introduction

Fractures, especially hip fractures, represent a growing health issue in aging societies. Recovery of mobility after such an injury can be prolonged and not always complete, affecting patients' quality of life. Modern medicine is exploring methods to support healing, including parathyroid hormone (PTH) therapy, which stimulates bone tissue formation, opening new perspectives in fracture treatment.

Aim of the study

The objective of this study is to review current scientific reports and summarize the existing knowledge on the impact of teriparatide supplementation on bone healing after fractures.

Materials and Methods

A review of randomized clinical trials (RCTs) published between 2016 and 2024 regarding the impact of teriparatide use on bone healing. Four studies meeting specific selection criteria were identified.

Results

The majority of analyzed studies demonstrated that the use of teriparatide improves bone mineral density. This effect was particularly pronounced among patients with prolonged teriparatide administration. In certain studies (e.g., those by Lee SY and Huang), a faster bone healing process and improved radiographic outcomes were observed in patients receiving teriparatide. Patients undergoing teriparatide supplementation reported reduced pain perception and improved mobility compared to control groups, suggesting a positive impact of the therapy on patients' quality of life during the recovery period.

Conclusions
The use of teriparatide shows potential as an effective therapy to enhance recovery after bone fractures.
INTELIGÊNCIA ARTIFICIAL GENERATIVA NA EDUCAÇÃO SUPERIOR: APORTES PARA UMA PRÁTICA PEDAGÓGICA CRÍTICO-REFLEXIVA
Authors: Santo Eniel do Espírito , Souza Sales Mary Valda , Carvalho Ottoni André Luiz 
Published: February 2025

A recente massificação da inteligência artificial generativa (IAG) vem causando um movimento crítico e reflexivo no campo da educação, tendo em vista as suas potencialidades que evidenciam desafiadoras questões. Considerando tal contexto, este estudo analisou os aportes crítico-reflexivos acerca da compreensão de IAG, expondo questões pedagógicas relacionadas com a formação e possibilidades didáticas, com foco no cenário da educação superior. Buscou-se, como indicação metodológica, desenvolver uma pesquisa exploratória e descritiva, de abordagem qualitativa, utilizando-se a pesquisa bibliográfica como suporte para o estudo de caso realizado no contexto do ensino superior, com 175 estudantes vinculados a um curso de formação continuada de professores, isto é, a pós-graduação lato sensu em Tecnologias e Educação Aberta e Digital, da Universidade Federal do Recôncavo da Bahia (UFRB). Em relação à inserção da IAG na prática pedagógica, 26 (16,2%) dos participantes consideraram indiferente, pouco oportuno ou não oportuno e 123 (76,4%) dos respondentes a consideram muito oportuna como dispositivo potencializador do processo educativo. As narrativas dos participantes revelam preocupação com a falta de conhecimento e lacunas formativas no desenvolvimento de competências e fluência digital. Contudo, as potencialidades de integração da IAG na prática pedagógica, evidenciadas nas narrativas dos participantes, remetem-nos a variadas possibilidades no contexto do processo de ensino e aprendizagem. O estudo conclui que a inserção da IAG na educação superior requer cuidadosa análise crítica e conhecimento das potencialidades dessa tecnologia, valorizando-se o contexto social, político e econômico e, sobretudo, estabelecendo-se um processo dialógico de reflexão crítica, alinhado com a promoção da formação emancipatória e cidadã.
Genetic evidence for amlodipine’s protective role in gastroesophageal reflux disease: A focus on CACNB2
Authors: Zhang Liuzhao , Chu Quanwang , Jiang Shuyue , Shao Bo 
Published: February 2025

Objective
This study aims to elucidate the causal relationship between genetically predicted amlodipine use and the risk of gastroesophageal reflux disease (GERD) using a bidirectional Mendelian Randomization (MR) approach and to explore the underlying genetic and molecular mechanisms through functional enrichment analysis and the construction of a competing endogenous RNA (ceRNA) network.

Methods
Publicly available GWAS datasets from the Neale Lab consortium were used, including data on amlodipine (13,693 cases, 323,466 controls) and GERD (14,316 cases, 322,843 controls). Genome-wide significant SNPs were selected as instrumental variables and clustered by linkage disequilibrium. MR analysis was conducted using R software with all five methods. Sensitivity analyses assessed pleiotropy and heterogeneity. Drug target genes were analyzed using GO and KEGG pathways. GeneMANIA was used for network visualization, and a ceRNA network was constructed with Cytoscape. Differential gene expression analysis on GERD-related datasets from GEO validated the findings.

Results
The MR analysis indicated a significant negative association between genetically predicted amlodipine use and GERD risk (IVW OR = 0.872, 95% CI = 0.812–0.937, P = 0.0002). Sensitivity analyses confirmed the robustness of these findings, showing no evidence of pleiotropy or heterogeneity. The enrichment analysis identified key biological processes and pathways involving calcium ion transport and signaling. The ceRNA network highlighted core targets such as CACNB2, which were further validated by differential expression analysis intersecting drug target genes with GERD-related gene expression changes.

Conclusion
This study provides robust evidence of a protective effect of amlodipine against GERD, supported by genetic and molecular analyses. The findings suggest that calcium channel blockers like amlodipine could be repurposed for GERD treatment. The identification of CACNB2 and other core targets in the ceRNA network offers novel insights into the pathophysiology of GERD and potential therapeutic targets, paving the way for personalized medicine approaches to improve patient outcomes.
Active tuberculosis in household contacts of bacteriologically confirmed pulmonary tuberculosis patients: A multicenter study finding the ‘Missed One’ in Central Ethiopia
Authors: Seid Getachew , Alemu Ayinalem , Diriba Getu , Hailu Michael , Wondimu Amanuel , Tadesse Mengistu , Tadesse Gemechu , H Mariam Solomon , Gumi Balako 
Published: February 2025

Background
There was a ‘missing millions’ gap between the incidence of tuberculosis (TB) cases and the notified cases. In many TB high-burden countries, only about 25% of household contacts (HHCs) completing household TB evaluation and 20–89% of eligible contacts did not adhere to TB screening. The study was conducted to assess the yield of door-to-door TB household contact investigation among household contact of bacteriologically confirmed pulmonary TB cases in central Ethiopia.

Methods
This cross-sectional study was carried out in selected health facilities of central Ethiopia from January 1, 2023 to December 3, 2023.All sequential voluntary bacteriologically confirmed TB patients and their HHCs without discrimination by age were included in the study. Xpert Ultra assay and TB culture were used to investigate active TB from sputum sample. Spearman’s correlation analysis was used to determine the correlation between the index case cycle threshold value and the corresponding HHCs. Multivariable logistic regression analysis was done to investigate the associated risk factors for active TB in HHCs.

Results
Among 967 HHCs claimed by 303 index cases (259 drug susceptible TB (DS-TB) and 44 multi-drug resistance TB (MDR/RR-TB)), 902(93.07%) HHCs had received baseline symptom-based TB evaluation. Presumptive TB was identified in 20.17% (182) of the evaluated HHCs and 13(1.44%) were diagnosed with active TB. Eleven HHCs (7.24%; 95% CI: 3.85–12.9%) from DS-TB index case contacts and 2 (6.67%; 95% CI: 1.16–23.51) from MDR/RR-TB indexes HHCs were found to be MTB detected Rifampicin resistance not detected cases. The Xpert ultra assay results revealed an 84.62% (95% CI: 57.77–95.68) Rifampicin drug resistance concordance between the index case and the corresponding HHC. Active TB was significantly associated with night sweating and sharing a bed with the index patient, P-value < 0.05.

Conclusion
Home-to-home TB contact screening have high active TB yield and implementable in both rural and urban areas of the nation only by mentoring and motivating the health extension workers. Proximity to bacteriologically confirmed TB patient for long time exposes household contacts for active TB. Scheduling convenient times and last-mile service delivery to contacts is very important to address the missed active TB cases in the community.