bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE REGULATION OF WHITE LOCUS EXPRESSION: A DOMINANT MUTANT ALLELE AT THE WHITE LOCUS OF DROSOPHILA MELANOGASTER
Authors: Bingham Paul M 
Published: April 2021

ABSTRACT
A new mutant allele (wDZL)at the white locus of Drosophila melanogaster is dominant to the wild-type allele, but apparently only when the two alleles are synapsed. When chromosomal rearrangements prevent somatic pairing between the two white alleles, wDZL is rendered recessive to wild type. This observation suggests that the dominance of wDZL is sensitive to a synapsis (transvection) effect. On the basis of this and other properties, it is proposed that wDZL causes the repression of transcription of a synapsed w+ allele, but not of a w+ allele elsewhere in the same nucleus. One model to account for this supposes that wDzL produces a repressor of white-locus transcription. This repressor is presumed to be so unstable that other white genes, removed from wDZL but in the same nucleus, are not detectably repressed. These properties may be simply understood if it is assumed that the repressor produced by the wDZL allele is an RNA molecule.
GENETIC EVIDENCE FOR A SILENT SUC GENE IN YEAST
Authors: Carlson Marian , Osmond Barbara C , Botstein David 
Published: April 2021

ABSTRACT
The SUC genes (SUC1-SUC7) of Saccharomyces are a family of genes that are dispersed in the yeast genome. A SUC + allele at any locus confers the ability to produce the enzyme invertase and, thus, to ferment sucrose. Most yeast strains do not carry SUC + alleles at all possible SUC loci. We have investigated the naturally occurring negative (suc0) alleles present at SUC loci with the aim of distinguishing between two possible models for the structure of suc0 alleles: (1) suc0 alleles correspond to a simple absence of SUC genetic information; (2) suc0 alleles are "silent" SUC genes that either produce a defective product or are not expressed. To facilitate these studies, sucrose-nonfermenting strains were constructed that are congenic to S. cerevisiae strain S288C (SUC2 +), but carry at the SUC2 locus the naturally occurring negative allele, suc20, of strain FL100 (Lacroute 1968). These strains were used to study the genetic properties of the suc20 allele of FL100 and the suc0 alleles (suc10, suc30, etc.) of S288C. The suc20 allele was shown to revert to an active Suc+ state and to provide functional information at three points in the SUC2 gene in recombination experiments; this suc20 gene thus appears to be a "silent" gene. Similar tests for silent SUC genes in S288C (corresponding to loci other than SUC2) failed to reveal any additional silent genes.
Nationwide audit and feedback on implementation of antibiotic stewardship programmes in Norwegian hospitals
Authors: Skodvin Brita , Høgli June U , Gravningen Kirsten , Neteland Marion I , Harthug Stig , Akselsen Per E 
Published: April 2021

Abstract

Background
Implementation of antibiotic stewardship programmes (ASPs) in hospitals is challenging and there is a knowledge gap on how to pursue this process efficiently.

Objectives
To evaluate whether audit and feedback (A&F) is a feasible and useful methodology to assess and support the implementation of ASPs in hospitals.

Methods
A multidisciplinary team performed document reviews and on-site interviews with professionals involved in the implementation of ASPs. Oral feedback on preliminary findings and areas of improvement were provided on-site, followed by feedback reports summarizing major findings and recommendations. Descriptive statistics were used to present number of hospital trusts, interviewees, professions, disciplines, workload and costs.

Results
All 22 hospital trusts in Norway participated in the A&F conducted October 2017 to April 2019. Altogether, 446 leaders and healthcare workers were interviewed: 110 leaders, 336 health professionals of whom 89 were antimicrobial stewardship team members. Median number of days from audits were performed till reporting were 36 (IQR 30–49). Median workload for auditors per visit was 7 days (6–8). Total costs were €133 952. Main audit findings were that ASP structures were established in most hospital trusts, but leadership commitment and implementation of interventions were often lacking. The hospital trusts received feedback on establishing governance structures, setting local targets, implementing interventions and increased involvement of nurses.

Conclusions
Nationwide A&F provides a unique and comprehensive insight into the implementation of ASPs in hospitals and is feasible with a reasonable amount of resources. This approach can identify targets for improved implementation of ASPs in hospitals.
Volvulus of the transverse colon in an octogenarian veteran
Authors: Pickett Maryanne L , Mottershaw Ann M , Gupta Pramod , Huerta Sergio 
Published: April 2021

Abstract
In the USA, the third leading cause of a large bowel obstruction in adults is volvulus with torsion occurring commonly in the sigmoid and the cecum. Transverse colonic volvulus is exceedingly rare and specific involvement of the splenic flexure is even more uncommon. In the present report, we discuss a Veteran octogenarian who presented with a long-standing history of constipation, but then developed an acute abdomen from a large bowel obstruction. At laparotomy, he had a double closed loop obstruction with volvulus of the splenic flexure. The colon at the splenic flexure was ischemic with patchy areas of necrosis, but no perforation. He underwent a subtotal colectomy with an ileostomy. This case illustrates the need for prompt intervention of this unusual entity. Current trends in the incidence, management, morbidity and mortality are discussed.
Primary posterior mediastinal angiolipoma: a case report
Authors: Nakamura Daisuke , Kobayashi Nobutaka , Miyazawa Masahisa , Satomi Hidetoshi , Ihara Nobumaro , Tsunoda Mayu 
Published: April 2021

Abstract
An angiolipoma is a benign tumor, and a primary mediastinal angiolipoma is extremely rare. Herein, we describe the presentation and management of a posterior mediastinal angiolipoma in a woman with loss of consciousness. Chest computed tomography (CT) revealed a contrast-enhancing mass in the right posterior mediastinum, with intercostal arterial blood supply identified on three-dimensional reconstruction CT (3D-CT). Magnetic resonance imaging revealed a fatty component. Pre-operative embolization of the supplying intercostal artery was performed to reduce intraoperative bleeding. Mass resection was performed using video-assisted thoracic surgery. Histopathology confirmed angiolipoma diagnosis. Although rare, a posterior mediastinum angiolipoma should be considered a possibility; 3D-CT and pre-operative embolization may be useful in the surgical treatment of hypervascular mediastinal tumors, such as angiolipomas.
Urban green spaces in Dhaka, Bangladesh, harbour nearly half the country’s butterfly diversity
Authors: Chowdhury Shawan , Shahriar Shihab A , Böhm Monika , Jain Anuj , Aich Upama , Zalucki Myron P , Hesselberg Thomas , Morelli Federico , Benedetti Yanina , Persson Anna S , Roy Deponkor K , Rahman Saima , Ahmed Sultan , Fuller Richard A 
Published: April 2021

AbstractCities currently harbour more than half of the world’s human population and continued urban expansion replaces natural landscapes and increases habitat fragmentation. The impacts of urbanisation on biodiversity have been extensively studied in some parts of the world, but there is limited information from South Asia, despite the rapid expansion of cities in the region. Here, we present the results of monthly surveys of butterflies in three urban parks in Dhaka city, Bangladesh, over a 3-year period (January 2014 to December 2016). We recorded 45% (137 of the 305 species) of the country’s butterfly richness, and 40% of the species detected are listed as nationally threatened. However, butterfly species richness declined rapidly in the three study areas over the 3-year period, and the decline appeared to be more severe among threatened species. We developed linear mixed effect models to assess the relationship between climatic variables and butterfly species richness. Overall, species richness was positively associated with maximum temperature and negatively with mean relative humidity and saturation deficit. Our results demonstrate the importance of urban green spaces for nationally threatened butterflies. With rapidly declining urban green spaces in Dhaka and other South Asian cities, we are likely to lose refuges for threatened fauna. There is an urgent need to understand urban biodiversity dynamics in the region, and for proactive management of urban green spaces to protect butterflies in South Asia.
Prolonged Sars-CoV-2 Viral Culture Detected in a Mild COVID-19 Infection
Authors: Vaught Eric S , Rogers Greg A , Ryan Keith E , Averitt Andrew W , Rice Jeffrey M 
Published: April 2021

ABSTRACT
We describe a case of prolonged shedding of live Severe Acute Respiratory Syndrome Coronavirus, 2 in the setting of a mild novel Coronavirus Disease 2019 (COVID-19) infection in a healthy young adult male. The patient did not require hospitalization or any supplemental oxygen. The viral cultures remained positive 15 days from symptom onset. The extended duration of positive viral culture with his concurrent mild symptomatology is at odds with current CDC and DOD isolation policies for positive COVID-19 patients. This case is important as health agencies around the world grapple with how to advise patients and create protocols for isolation precautions after positive COVID-19 results.
Analytical solutions for radiation-driven winds in massive stars – II. The δ-slow regime
Authors: Araya I , Christen A , Curé M , Cidale L S , Venero R O J , Arcos C , Gormaz-Matamala A C , Haucke M , Escárate P , Clavería H 
Published: April 2021

ABSTRACT
Accurate mass-loss rates and terminal velocities from massive stars winds are essential to obtain synthetic spectra from radiative transfer calculations and to determine the evolutionary path of massive stars. From a theoretical point of view, analytical expressions for the wind parameters and velocity profile would have many advantages over numerical calculations that solve the complex non-linear set of hydrodynamic equations. In a previous work, we obtained an analytical description for the fast wind regime. Now, we propose an approximate expression for the line-force in terms of new parameters and obtain a velocity profile closed-form solution (in terms of the Lambert W function) for the δ-slow regime. Using this analytical velocity profile, we were able to obtain the mass-loss rates based on the m-CAK theory. Moreover, we established a relation between this new set of line-force parameters with the known stellar and m-CAK line-force parameters. To this purpose, we calculated a grid of numerical hydrodynamical models and performed a multivariate multiple regression. The numerical and our descriptions lead to good agreement between their values.
Conspecific attraction for conservation and management of terrestrial breeding birds: Current knowledge and future research directions
Authors: Valente Jonathon J , LeGrande-Rolls Christa L , Rivers James W , Tucker Anna M , Fischer Richard A , Betts Matthew G 
Published: April 2021

Abstract
Conspecific presence can indicate the location or quality of resources, and animals settling near conspecifics often gain fitness benefits. This can result in adaptive conspecific attraction during breeding habitat selection as demonstrated in numerous terrestrial, territorial birds. There is growing interest in using simulated conspecific social cues (e.g., decoys, broadcasted vocalizations) to manage bird distributions, yet it remains unclear when this approach is likely to succeed. We reviewed published studies to evaluate whether the strength of conspecific attraction in terrestrial birds is mediated by characteristics of species (life history traits), simulated cues (e.g., timing and duration), sites (e.g., quality), and how conspecific attraction was measured. We identified 31 experiments that simulated social cues and compared conspecific settlement between treatment and control sites. We then used phylogenetically controlled meta-regression to assess the impacts of 19 moderators on settlement. Nearly all species included in these experiments were migratory passerines, and social cues generally had a strong, positive influence on their settlement decisions, as the odds of site occupancy were 3.12× (95% CI: 0.81–11.69) greater in treatment sites relative to control sites. Within this group, conspecific attraction was evolutionarily conserved with ≥25.5% (95% CI: 5.1%–65.4%) of the variance in treatment effects explained by phylogenetic relatedness. However, we found no evidence that any covariates influenced the response to social cues, and we posit this stems from limited research specifically designed to identify the mechanisms mediating conspecific attraction. We therefore developed a research agenda that provides a framework for testing mechanistic hypotheses regarding how cue characteristics, species traits, and spatial contexts may mediate attraction to conspecifics. Evaluating these hypotheses will greatly advance the field by helping managers understand when, where, and why simulating social cues can be used to enhance populations of species that are of conservation concern.
Should Robots Be Taxed?
Authors: Guerreiro Joao , Rebelo Sergio , Teles Pedro 
Published: April 2021

Abstract
Using a quantitative model that features technical progress in automation and endogenous skill choice, we show that, given the current U.S. tax system, a sustained fall in automation costs can lead to a massive rise in income inequality. We characterize the optimal tax system in this model. We find that it is optimal to tax robots while the current generations of routine workers, who can no longer move to non-routine occupations, are active in the labour force. Once these workers retire, optimal robot taxes are zero.
Joint anatomy in axial spondyloarthritis: strong associations between sacroiliac joint form variation and symptomatic disease
Authors: Ziegeler Katharina , Kreutzinger Virginie , Proft Fabian , Poddubnyy Denis , Hermann Kay Geert A , Diekhoff Torsten 
Published: April 2021

Abstract

Objectives
The aim of this investigation was to determine the prevalence of variant SI joint forms in symptomatic patients with mechanical joint disease (MJD) and axial SpA (axSpA) compared with control patients.

Method
A total of 973 patients were included in this cross-sectional study (95 axSpA; 61 MJD; 817 controls); clinical diagnosis, age and gender were noted. An established scoring system was used to classify joint forms on CT scans of the pelvis. Frequencies of joint forms were compared between groups (axSpA; MJD; controls).

Results
Patients with MJD exhibited the highest proportion of atypical joints (80.3% in MJD vs 44.1% in axSpA and 37.5% in controls; P &lt; 0.001). Overall, females had a significantly higher proportion of atypical joints than men (65.0 vs 17.8%; P &lt; 0.001); proportions of atypical joints were significantly higher in males with symptomatic joint disease than in male controls: 32.2% in axSpA, 55.0% in MJD and 13.9% in controls; P ≤ 0.001. Two specific joint forms were significantly more prevalent in symptomatic patients than in controls: the iliosacral complex (16.2 vs 4.2%; P &lt; 0.001) and the crescent-shaped ilium (9.1 vs 2.8%; P &lt; 0.05).

Conclusions
Our data demonstrate a strong association between atypical joint forms and symptomatic joint disease.
Scoring of large muscle group movements during sleep: an International Restless Legs Syndrome Study Group position statement
Authors: Ferri Raffaele , DelRosso Lourdes M , Provini Federica , Stefani Ambra , Walters Arthur S , Picchietti Daniel L 
Published: April 2021

Abstract
There is a gap in the manuals for scoring sleep-related movements because of the absence of rules for scoring large movements. A taskforce of the International Restless Legs Syndrome Study Group (IRLSSG) elaborated rules that define the detection and quantification of movements involving large muscle groups. Consensus on each of the criteria in this article was reached by testing the presence of consensus on a first proposal; if no consensus was achieved, the concerns were considered and used to modify the proposal. This process was iterated until consensus was reached. A preliminary analysis of the duration of movements involving large muscle groups was also carried out on data from two previous studies, which, however, used a visual analysis of video-polysomnographic (PSG) recordings obtained from children or adults. Technical specifications and scoring rules were designed for the detection and quantification of large muscle group movements during sleep with a duration between 3 and 45 seconds in adults or 3 and 30 seconds in children, characterized by an increase in electromyographic activity and/or the occurrence of movement artifact in any combination of at least two recommended channels and not meeting the criteria for any other type of movement. Large muscle group movements are often accompanied by sleep stage changes, arousals, awakenings, and heart rate rises. The absence of clear and detailed rules defining them has likely impeded the development of studies that might disclose their clinical relevance; these new rules fill this gap.
Aerobic fitness and the sleeping brain of adolescents—a pilot study
Authors: Neikrug Ariel B , Mander Bryce A , Radom-Aizik Shlomit , Chen Ivy Y , Stehli Annamarie , Lui Kitty K , Chappel-Farley Miranda G , Dave Abhishek , Benca Ruth M 
Published: April 2021

AbstractStudy ObjectivesAerobic fitness (AF) and sleep are major determinants of health in adolescents and impact neurocognitive and psychological development. However, little is known about the interactions between AF and sleep during the developmental transition experienced across adolescence. This study aimed to consider the relationships between AF and habitual sleep patterns and sleep neurophysiology in healthy adolescents.MethodsSubjects (mean age = 14.6 ± 2.3 years old, range 11–17, 11 females) were evaluated for AF (peak VO2 assessed by ramp-type progressive cycle ergometry in the laboratory), habitual sleep duration and efficiency (7–14 days actigraphy), and topographic patterns of spectral power in slow wave, theta, and sleep spindle frequencies in non-rapid eye movement (NREM) sleep using overnight polysomnography (PSG) with high-density electroencephalography (hdEEG, 128 channels).ResultsSignificant relationships were observed between peak VO2 and habitual bedtime (r = −0.650, p = .009) and wake-up time (r = −0.603, p = .017), with greater fitness associated with going to bed and waking up earlier. Peak VO2 significantly predicted slow oscillations (0.5–1 Hz, p = .018) and theta activity (4.5–7.5 Hz, p = .002) over anterior frontal and central derivations (p &lt; .001 and p = .001, respectively) after adjusting for sex and pubertal development stage. Similar associations were detected for fast sleep spindle activity (13–16 Hz, p = .006), which was greater over temporo-parietal derivations.ConclusionsGreater AF was associated with a more mature pattern of topographically-specific features of sleep EEG known to support neuroplasticity and cognitive processes and which are dependent on prefrontal cortex and hippocampal function in adolescents and adults. AF was also correlated with a smaller behavioral sleep phase delay commonly seen during adolescence.
Metagenomic sequencing reveals a lack of virus exchange between native and invasive freshwater fish across the Murray–Darling Basin, Australia
Authors: Costa Vincenzo A , Mifsud Jonathon C O , Gilligan Dean , Williamson Jane E , Holmes Edward C , Geoghegan Jemma L 
Published: April 2021

Abstract
Biological invasions are among the biggest threats to freshwater biodiversity. This is increasingly relevant in the Murray–Darling Basin, Australia, particularly since the introduction of the common carp (Cyprinus carpio). This invasive species now occupies up to ninety per cent of fish biomass, with hugely detrimental impacts on native fauna and flora. To address the ongoing impacts of carp, cyprinid herpesvirus 3 (CyHV-3) has been proposed as a potentially effective biological control agent. Crucially, however, it is unknown whether CyHV-3 and other cyprinid herpesviruses already exist in the Murray–Darling. Further, little is known about those viruses that naturally occur in wild freshwater fauna, and the frequency with which these viruses jump species boundaries. To document the evolution and diversity of freshwater fish viromes and better understand the ecological context to the proposed introduction of CyHV-3, we performed a meta-transcriptomic viral survey of invasive and native fish across the Murray–Darling Basin, covering over 2,200 km of the river system. Across a total of thirty-six RNA libraries representing ten species, we failed to detect CyHV-3 nor any closely related viruses. Rather, meta-transcriptomic analysis identified eighteen vertebrate-associated viruses that could be assigned to the Arenaviridae, Astroviridae, Bornaviridae, Caliciviridae, Coronaviridae, Chuviridae, Flaviviridae, Hantaviridae, Hepeviridae, Paramyxoviridae, Picornaviridae, Poxviridae, Reoviridae and Rhabdoviridae families, and a further twenty-seven that were deemed to be associated with non-vertebrate hosts. Notably, we revealed a marked lack of viruses that are shared among invasive and native fish sampled here, suggesting that there is little virus transmission from common carp to native fish species, despite co-existing for over fifty years. Overall, this study provides the first data on the viruses naturally circulating in a major river system and supports the notion that fish harbour a large diversity of viruses with often deep evolutionary histories.
Assessing the Capacity to Work Among Older Workers: A Survival Analysis of Retirement Behavior
Authors: Boissonneault Michaël , de Beer Joop 
Published: April 2021

Abstract
Whether increases to statutory retirement ages will have the anticipated effect in countries of the Organization for Economic Co-operation and Development (OECD) depends on whether workers have the health capacity to postpone retirement. Methods that were proposed to measure the capacity to work at older age are ill-designed to assess inter-cohort trends, which is important for determining whether the amount of years spent retired and in good health is keeping up with increases in the statutory retirement age. We propose to measure the capacity to work as the amount of time that people would spend working if they kept working until poor health forces them to retire. We find that American workers born in 1936–1947 spent 9.4 years working between ages 55 and 69 but had the capacity to work an additional 3.3 years. We further find significant inter-cohort increases in the years spent working but insignificant ones in the years spent able to work, which might point toward a decrease in the years spent retired and in good health. Increases in the educational attainment of younger cohorts have had a positive effect on the capacity to work, but the expansion of obesity a negative one. Finally, we find similar trends among men and women as well as among Whites and non-Whites, although the capacity to work is much lower among non-Whites. Our results show the importance of considering inter-cohort changes in the capacity to work when designing policies that aim at inducing higher retirement ages.