bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosome length genome assembly of the redbanded stink bug, Piezodorus guildinii (Westwood).
Authors: Perera Omaththage P. 
Published: September 2021

Abstract
Objective: The redbanded stink bug, Piezodorus guildinii (Hemiptera: Pentatomidae), is native to Caribbean Basin and is currently considered an invasive pest in Florida, Louisiana, Mississippi, and Texas in the southern United States. Although P. guildinii is an economically important invasive pest in the USA, a relatively few studies have been conducted to understand genetic, population genetic structure, and genetic basis of resistance to insecticides. The objective of this work was to obtain a high-quality genome assembly to develop genetic resources to conduct genetic, population genetic, and physiological studies of the RBSB. Results: The genome of RBSB was sequenced with Pacific Biosciences technology followed by two rounds of scaffolding using Chicago libraries and HiC proximity ligation to obtain a high-quality assembly. The genome assembly contained 800 scaffolds larger than 1 kbp and the N50 was 170.84 Mbp. The largest scaffold was 222.22 Mbp and 90% of the genome was included in the 7 scaffolds larger than 1 Mbp. The number of megabase scaffolds also matched the number of chromosomes in this insect. The genome sequence will facilitate the development of resources to conduct studies on genetics, transcriptomics, and physiology of RBSB.
Effectiveness of standardized peri-operative protocol with combination of gentamicin and levofloxacin as prophylactic antibiotics on preventing infections after transrectal prostate biopsy: A retrospective study of 577 patients
Authors: Chiang Heng-Chieh , Lin Jesun , Yan Meng-Yi , Chen Chun-Chi , Chen Jian-Ting , Chen Pao-Hwa 
Published: September 2021

Abstract
PurposeThis retrospective study to determine the effectiveness standardized peri-operative protocol in combination with prophylactic gentamicin and levofloxacin in preventing infectious complications after trans-rectal prostate biopsy.Material and MethodPatients were screened for prostate cancer at our out-patient department, either due to abnormal digital examination or elevated PSA level. Patients who underwent transrectal ultrasound (TRUS) guided prostate biopsy from January 2008 to December 2012 was included in this study. After using surgical code to identify the patients, charts were reviewed, and complications were recorded. Infectious complications were defined as any patient who experienced any signs systemic inflammatory response syndrome (SIRS) within 14 days after undergoing TRUS biopsy. Complications were graded according to Clavien-Dindo classification.ResultsOut of the 577 patients there were 20 patients (3.47 %) with infectious complications. Of the 20 patients with infectious complications, only four patients (0.7%) needed hospitalization due to bacteremia. Minor complications (i.e., hematuria, acute urine retention, hematospermia, etc.) were self-resolving. There were 9 positive urine culture and 4 positive blood culture with Escherichia coli (E. coli) as the predominant species. Three patients had positive urine culture for ciprofloxacin resistant strain, which was susceptible to 2nd an 3rd generation cephalosporin or amikacin. One patient had blood culture positive for extended-spectrum beta-lactamase (ESBL) E. coli infection, which was sensitive to amikacin. The infectious complication rate and number decrease each year without increase in resistant strain.ConclusionOur current peri-operative and post-operative protocol appears to be feasible in reducing infection complications after prostate biopsy; our complication and sepsis rate were similar compared to other English literatures. A prospective randomized controlled trial would be needed to determine if a single factor or a combination of several factors are responsible for the reduction in post-biopsy infections.
Major Mistakes and Errors in the Use of Trial Sequential Analysis in Systematic Reviews or Meta-analyses – Protocol for a Systematic Review
Authors: Riberholt Christian Gunge , Olsen Markus Harboe , Milan Joachim Birch , Gluud Christian 
Published: September 2021

Abstract
Background: Adequately conducted systematic reviews with meta-analyses are considered the highest level of evidence and thus directly defines many clinical guidelines. However, the risk of type I and II errors in meta-analyses are substantial. Trial Sequential Analysis is a method for controlling these risks. Erroneous use of the method might lead to research waste or misleading conclusions. Methods: The current protocol describes a systematic review aimed to identify common and major mistakes and errors in the use of Trial Sequential Analysis by evaluating published systematic reviews and meta-analyses that include this method. We plan to include all studies using Trial Sequential Analysis published from 2018 to 2021, an estimated 400 to 600 publications. We will search Medical Literature Analysis and Retrieval System Online (MEDLINE) and the Cochrane Database of Systematic Reviews (CDSR), including studies with all types of participants, interventions, and outcomes. The search will begin in July 2021. Two independent reviewers will screen titles and abstracts, include relevant full text articles, extract data from the studies into a predefined checklist, and evaluate the methodological quality of the study using the AMSTAR 2 (Assessing the methodological quality of systematic reviews). Discussion: This protocol follows the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P). The identified mistakes and errors will form the basis of a reviewed guideline for the use of Trial Sequential Analysis. Appropriately controlling for type I and II errors might reduce research waste and improve quality and precision of the evidence that clinical guidelines are based upon.
RIT1 Promotes Glioma Proliferation and Invasion via the NF-ĸB/p65 pathway
Authors: Che Minggang , Lan Qing 
Published: September 2021

Abstract
RIT1, a member of the Ras family, has been identified as an oncogene in several malignancies. However, the expression and function of RIT1 in glioma remains to be addressed. In this study, we found RIT1 was upregulated in glioma and was associated with poor prognosis of glioma patients. Manipulating RIT1 levels in glioma cells via RNA interference significantly inhibited glioma cell proliferation and invasion in vitro whereas RIT1 overexpression exhibited the opposite effects. Mechanistically, we demonstrate that RIT1 engaged in the activation of the NF-ĸB pathway in vitro and in vivo. Furthermore, treating RIT1-overexpressing glioma cells with the p65 siRNA partially restrained their proliferation and invasion. Together these results indicate RIT1 contributes to the development and metastasis of glioma via the NF-ĸB pathway and suggest that targeting RIT1 may be a treatment strategy for this disease.
Intelligent Fault Diagnosis Based on Dynamic Convolutional Depth Domain Adaptive Network
Authors: Zhou Huafeng , Cheng Pei-Yuan , Shao Si-Yu , Zhao Yu-Wei , Yang Xin-Yu 
Published: September 2021

Abstract
Deep learning-based mechanical fault diagnosis method has made great achievements. A high-performance neural network model requires sufficient labelled data for training to obtain accurate classification results. Desired results mainly depends on assumption that training and testing data are collected under the same working conditions, environment and operating conditions, where the data have the same probability distribution. However, in the practical scenarios, training data and the testing data follow different distributions to some degree, and the newly collected testing data are usually unlabeled. In order to solve the problems above, a model based on transfer learning and domain adaptation is proposed to achieve efficient fault diagnosis under different data distributions. The proposed framework adapts the features extracted by multiple dynamic convolutional layers, and creatively utilizes correlation alignment(CORAL) to perform a non-linear transformation to align the second-order statistics of the two distributions for fault diagnosis, which greatly improves the accuracy of fault classification in the target domain under unlabeled data. Finally, experimental verifications have been carried out among two different datasets.
Ailanthone decreases cell viability in tongue squamous cell carcinoma via PI3K/AKT pathway
Authors: wang shuhan , Chen Xiaoyu , Zhu Xuejie , Lin Kehao , Cui Qixiao , Zheng Qiusheng , Li Defang , Wang Yuliang 
Published: September 2021

Abstract
To investigate the anti-tumor effect and mechanisms of ailanthone (AIL) in tongue squamous cell carcinoma (TSCC). The viability and apoptotic cell number of TCA8113 and Cal-27 cells declined and increased considerably following AIL. Hoechst 33258 staining revealed chromatin aggregation after exposure to AIL. Along with an accumulation of cleaved caspase-9, caspase-3, and PARP1, Bcl-2/Bax ratio and the levels of caspase-3, caspase-9 and PARP1 reduced after exposure to AIL treatment. Subjecting Cal-27 cells to AIL treatment led to the arrestment of the cell cycle at the G2/M phase. Nevertheless, the cell cycle of AIL-treated TCA8113 cells did not change significantly. Following AIL treatment, a decline in the expression of CDK1 and cyclin B1 was manifested by Western Blot. The p-AKT as well as the expression level of p-PI3K underwent a significant downregulation by AIL in both cells. The outcome furnishes a valuable understanding of the potential applications of AIL in treating TSCC.
Bacterial community shift and effects of the inclusion of a mixed psychrotrophic bacteria strain for sewage treatment in constructed wetland in winter seasons
Authors: xu xiaoyan , Jiang Jie , Guo Zhinan , Sun Lianglun , Tang Meizhen 
Published: September 2021

Abstract
The mechanism of wastewater treatment based on psychrophilic strains to improve the denitrification efficiency of constructed wetlands at low temperatures has already become a new hotspot. In this study, three mixed psychrophilic strains (Psychrobacter TM-1, Sphingobacterium TM-2 and Pseudomonas TM-3) with high capacity of denitrification were added into a vertical-flow constructed wetlands (CWs), and the effect of the mixed strains on CWs sewage treatment was evaluated. The removal efficiency of chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), total nitrogen (TN) and total phosphorus (TP) was quantified to establish the degradation kinetic model and determine the best dosage of the mixed strains. The effect mechanism of the mixed strains on indigenous microbial community and the change of sewage treatment performance in low temperature constructed wetlands was clarified by high-throughput sequencing technology. The results showed that the mixed strains can effectively remove the organic pollutants (nitrogen and phosphorus) and the optimum dosage of the mixed strain was 2.5%,with average removal rates of 1.52, 2.12, 2.07 and 1.29 times than those of the control. Meanwhile, the dominant strains in the CWs were Proteobacteria (31.23–44.34%), Chloroflexi (12.04–19.05%), Actinobacteria (10.6-20.62%), Acidobacteria (8.23–11.65%), Firmicutes (2.23–15.95%) and Bacteroidetes (4.01–18.9%). These findings provide a basis for the removal of pollutants in constructed wetlands at low temperature.
Synthesis and characterization poly (2- formylpyrrole) (PFPy) by Acids catalysis and study of its particles size
Authors: Al-Hamdan ahmad , Al-Falah Ahmad , Al-Deric Fawaz , Al-ghoraibi Ibrahim 
Published: September 2021

Abstract
In this paper Poly (2-formyl pyrrole) (PFPy) was synthesized using hydrochloric acid as catalysis in alcohol. PFPy is green dark very fine powder. Then the polymer forms glass substrate in the reaction mixture. The resulting polymer was characterized by (FT/IR, EDX and XPS) to determine the polymer structure. The polymer was scanned by scanning electron microscope (SEM) and its film by AFM for its morphological properties. We found the polymer consisting of spherical particles with a rough surface (average diameters of 430 nm) and they formed clusters. We proposed a method for calculated particles size depending on the crystals size (by Scherrer equation) and percentage of crystallization of polymer from XRD. The average particles size is 336.7 nm. The particles size in this method may be closer to reality because the XRD includes a large number of particles and it not optional as SEM and AFM.
Eardrum perforation treatment blood plasma enriched with platelet growth factors
Authors: Kriukov A. I. , Gurov A. V. , Ermolaev A. G. , Murzakhanova Z. V. , Dubovaya T. K. , Bakhtin A. A. 
Published: September 2021

Introduction. The problem of the development of acute post-traumatic otitis media has not lost its relevance. Long-term preservation of the defect of the tympanic membrane contributes to the development of chronic inflammation in the middle ear and a deterioration in the quality of life of patients.Objective. To study the effect of blood plasma enriched with platelet growth factors on the regeneration of the tympanic membrane tissues in patients with acute post-traumatic perforation.Materials and methods. Patients with acute post-traumatic perforation of the tympanic membrane were divided into the main (24 people) and control (19 people) groups. Patients of the main group in the area of perforation of the tympanic membrane were injected once by application with a clot of autoplasma, enriched with platelet growth factors. The control group patients underwent dynamic observation of the processes of natural regeneration of the tympanic membrane tissues. On the 5, 10 and 15th days, the tissue regeneration of the tympanic membranes was assessed subjectively (patient complaints, audiometry) and objectively (otoscopy with the calculation of the dynamics of the relative perforation area, otoacoustic emission).Results. A single application of blood plasma enriched with platelet growth factors to the perforation area was significantly more often accompanied by closure of the tympanic membrane perforation (p ≤ 0.01) and a decrease in the average relative area of the tympanic membrane perforation on days 10 and 15 of the study (p ≤ 0.01). The dynamics of the average relative area of the tympanic membrane perforation reflected a high rate of closure of the tympanic membrane defect in the main group. Better healing of the tympanic membrane in patients of the main group was accompanied by an improvement in the auditory function of the affected ear. The quality of hearing was, on average, significantly better in the patients of the main group than in the patients of the control group, both on the 10th and 15th days of observation (p ≤ 0.01).Conclusion. Clinical use of blood plasma enriched with platelet growth factors has a beneficial effect on the rate, intensity of closure of the tympanic membrane perforation and hearing.
Assessment of population health losses due to nasal obstruction based on the concept of the global burden of disease: general approaches and research directions
Authors: Pavlova S. S. , Korneenkov A. A. , Dvorianchikov V. V. , Ryazantsev S. V. , Ryazantseva E. S. , Donskaya O. S. 
Published: September 2021

The current impact of disease on society and the health care system is increasingly driven by disability rather than premature mortality. In otorhinolaryngology, there are many diseases that have a high prevalence and cause significant health losses: hearing loss, chronic tonsillitis and pharyngitis, and more. Nasal obstruction is a common symptom in clinical practice. Given the high prevalence of nasal congestion, a significant decline in quality of life, and a significant socio-economic burden in society, this symptom is often key in the treatment of patients with rhinological diseases and a significant factor in assessing the medical need for effective treatment options for this condition. The resolution of the Council of experts of the National Medical Association of Otorhinolaryngologists on problems of nasal obstruction (April 17, 2021, Sochi) states that «it is necessary to conduct a medical and statistical study of public health losses associated with diseases that cause nasal obstruction, using methods for assessing the global burden of the disease and risk factors for the development of nasal obstruction». As you know, the World Health Organization measures the global burden of disease in the number of years of life lost as a result of disability. This indicator combines years of life lost due to premature mortality and years of life lost due to health conditions that do not meet the criteria for full health. The assessment of the disease burden in our country is used quite actively, most often for diseases that lead to death. The assessment of the disease burden indicator for diseases accompanied by nasal obstruction in our country has not been carried out, although the problem of treating nasal obstruction is of high relevance. The aim of the study is to discuss issues related to the study of public health losses due to diseases that cause nasal obstruction, using methods for assessing the global burden of the disease. The article provides an overview of the causes of nasal obstruction, information about its prevalence in various diseases, and discusses the calculation of the burden of nasal obstruction and possible approaches to calculating the global burden of disease, taking into account current methodological problems in this area.
Pathogenetic bases of the use of antifibrotic therapy with Bovhyaluronidazum azoximerum in patients with new coronavirus infection COVID-19
Authors: Chernyavskaya O. A. , Osipov A. V. 
Published: September 2021

One of the most likely and serious complications of the novel coronavirus infection (COVID-19) is pneumofibrosis, which can negatively affect the duration and quality of life of patients who have suffered from this disease. The appearance of fibrotic changes in COVID-19 is due to a number of pathological processes that occur in the lungs after the pathogen, the SARS- CoV-2 virus, enters there. First of all, an inflammatory response is triggered, which is mediated by macrophages and granulocytes, due to which the synthesis of pro-inflammatory cytokines, incl. IL-1, TNF, which are potent inducers of hyaluronic acid synthetase. There is a decrease in the content of fibrinolysis activators in the pulmonary endothelium, which contributes to the accumulation of fibrin in the vessels of the lungs. Fibrin can escape into the interstitial space and cause the formation of sclerosing alveolitis. The increasing defeat of pneumocytes favors the release of fibrin into the lumen of the alveoli, which causes the formation of hyaline membranes. The regulation of the fibrotic process involves immunocompetent cells, primarily CD4 + T-lymphocytes, which are capable of producing cytokines, chemokines and growth factors, and these, in turn, stimulate the proliferation and differentiation of fibroblasts, as well as their production of collagen. The more severe forms of infectious process can lead to the greater risk of developing fibrotic changes. Risk factors are a large area of lung damage, the use of artificial pulmonary ventilation, ARDS, fibrosis in anamnesis. An additional role in the pathomorphogenesis of pneumofibrosis is played by smoking, external inhalation effects (inhalation of organic and inorganic dust), gastroesophageal reflux, type II diabetes mellitus, genetic factors (familial idiopathic pulmonary fibrosis). The pathogenetic features of COVID-19 require administration of anti-fibrotic treatment. Bovhyaluronidazum azoximerum, a drug that is a conjugate of the proteolytic enzyme hyaluronidase, is considered as an antifibrotic agent. The treatment regimens with this drug recommended in the period of convalescence for patients who have undergone COVID-19 are given.
Study of the medicine MestaMidin®-nos virucidal activity of against influenza and ARD pathogens
Authors: Shtro A. A. , Galochkina A. V. , Nikolaeva Yu. V. , Petukhova G. D. , Fedorova V. A. , Lioznov D. A. 
Published: September 2021

Introduction. Respiratory diseases have always been a serious threat to public health, but in 2020 the situation deteriorated significantly due to the rapid development of the coronavirus infection COVID-19. Due to the lack of available means of ethiotropic therapy and the still insufficient coverage of the general population with vaccination, disinfectants, as well as topical preparations that prevent the penetration of the virus into the body, play an important role in preventing the spread of infection.Aim of the study. This work is devoted to the study of the virucidal activity of the medicine Mestamidin-nos against respiratory viruses, namely influenza A and B viruses, parainfluenza, respiratory syncytial, adenovirus and seasonal coronavirus.Materials and methods. This study was carried out by the suspension method according to MU 3.5.2431-08 “Study and assessment of the virucidal activity of disinfectants”.Results. It was shown that for all studied viruses the use of the compound for 60 minutes led to a complete (up to 0 lgTID50) or sufficient (4 lgTID50) decrease in the viral titer. In the case of a study of a compound with a protein load (imitating strong organic pollution), the effectiveness of the compound was significantly reduced against influenza A viruses, one of the strains of influenza B virus, adenovirus and seasonal coronavirus.Conclusion. Based on the results of the study, it can be concluded that the effective inhibition of pathogens of influenza and acute respiratory infections using the compound MesaMidin®-nos in the absence of strong organic pollution.
Glycemic control as an important tool in the prevention of cardiovascular aging in patients with diabetes mellitus
Authors: Shatskaya O. A. , Bondarenko I. Z. , Kukharenko S. S. 
Published: September 2021

In recent decades, there has been a significant increase in the incidence and prevalence of diabetes mellitus. Diabetes mellitus is characterized by the development of vascular complications leading to early disability and a decrease in the life expectancy of patients. Aging of the body inevitably leads to the occurrence of age-related diseases, including the cardiovascular system, and increases the risk of death. Metabolic and structural and functional disorders of the cardiovascular system arising in diabetes mellitus have common pathophysiological mechanisms with aging of the body. Chronic hyperglycemia can accelerate the aging process and play a decisive role in the occurrence and prognosis of cardiovascular events in patients with diabetes mellitus. Achieving target glycemic values is an important step towards preventing vascular complications in patients with diabetes mellitus. Improved models of glucometers, equipped with a number of additional functions, allow for structured self-control of glycemia, analyze the data obtained and carry out timely correction of therapy, actively involve patients in the process of diabetes management, which will significantly increase the efficiency of disease management, reduce the risk of complications in patients and improve the quality of life.