bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global, regional, and national quality of care of ischaemic heart disease from 1990 to 2017: a systematic analysis for the Global Burden of Disease Study 2017
Authors: Aminorroaya Arya , Yoosefi Moein , Rezaei Negar , Shabani Mahsima , Mohammadi Esmaeil , Fattahi Nima , Azadnajafabad Sina , Nasserinejad Maryam , Rezaei Nazila , Naderimagham Shohreh , Ahmadi Naser , Ebrahimi Hooman , Mirbolouk Mohammadhassan , Blaha Michael J , Larijani Bagher , Farzadfar Farshad 
Published: April 2021

AbstractAimsBy 2030, we seek to reduce premature deaths from non-communicable diseases, including ischaemic heart disease (IHD), by one-third to reach the sustainable development goal (SDG) target 3.4. We aimed to investigate the quality of care of IHD across countries, genders, age groups, and time using the Global Burden of Diseases Study (GBD) 2017 estimates.Methods and resultsWe did a principal component analysis on IHD mortality to incidence ratio, disability-adjusted life-years (DALYs) to prevalence ratio, and years of life lost to years lived with disability ratio using the results of the GBD 2017. The first principal component was scaled from 0 to 100 and designated as the quality of care index (QCI). We evaluated gender inequity by the gender disparity ratio (GDR), defined as female to male QCI. From 1990 to 2017, the QCI and GDR increased from 71.2 to 76.4 and from 1.04 to 1.08, respectively, worldwide. In the study period, countries of Western Europe, Scandinavia, and Australasia had the highest QCIs and a GDR of 1 to 1.2; however, African and South Asian countries had the lowest QCIs and a GDR of 0.8 to 1. Moreover, the young population experienced more significant improvements in the QCI compared to the elderly in 2017.ConclusionFrom 1990 to 2017, the QCI of IHD has improved; nonetheless, there are remarkable disparities between countries, genders, and age groups that should be addressed. These findings may guide policymakers in monitoring and modifying our path to achieve SDGs.
dpy-18 Encodes an α-Subunit of Prolyl-4-Hydroxylase in Caenorhabditis elegans
Authors: Hill Katherine L , Harfe Brian D , Dobbins Carey A , L'Hernault Steven W 
Published: April 2021

Abstract
Collagen is an extracellular matrix (ECM) component encoded by a large multigene family in multicellular animals. Procollagen is post-translationally modified by prolyl-4-hydroxylase (EC 1.14.11.2) before secretion and participation in ECM formation. Therefore, collagen processing and regulation can be studied by examining this required interaction of prolyl-4-hydroxylase with procollagen. High-resolution polymorphism mapping was used to place the Caenorhabditis elegans dpy-18 gene on the physical map, and we show that it encodes a prolyl-4-hydroxylase α catalytic subunit. The Dpy phenotype of dpy-18(e364) amber mutants is more severe when this mutation is in trans to the noncomplementing deficiency tDf7, while the dpy-18(e499) deletion mutant exhibits the same phenotype as dpy-18(e499)/tDf7. Furthermore, dpy-18 RNA interference (RNAi) in wild-type worms results in Dpy progeny, while dpy-18 (RNAi) in dpy-18(e499) mutants does not alter the Dpy phenotype of their progeny. These observations suggest that the dpy-18 null phenotype is Dpy. A dpy-18::gfp promoter fusion construct is expressed throughout the hypodermis within the cells that abundantly produce the cuticle collagens, as well as in certain head and posterior neurons. While prolyl-4-hydroxylase has been studied extensively by biochemical techniques, this is the first report of a mutationally defined prolyl-4-hydroxylase in any animal.
kurtz, a Novel Nonvisual Arrestin, Is an Essential Neural Gene in Drosophila
Authors: Roman Gregg , He Jin , Davis Ronald L 
Published: April 2021

Abstract
The kurtz gene encodes a novel nonvisual arrestin. krz is located at the most-distal end of the chromosome 3R, the third gene in from the telomere. krz is expressed throughout development. During early embryogenesis, krz is expressed ubiquitously and later is localized to the central nervous system, maxillary cirri, and antennal sensory organs. In late third instar larvae, krz message is detected in the fat bodies, the ventral portion of the thoracic-abdominal ganglia, the deuterocerebrum, the eye-antennal imaginal disc, and the wing imaginal disc. The krz1 mutation contains a P-element insertion within the only intron of this gene and results in a severe reduction of function. Mutations in krz have a broad lethal phase extending from late embryogenesis to the third larval instar. The fat bodies of krz1 larva precociously dissociate during the midthird instar. krz1 is a type 1 melanotic tumor gene; the fat body is the primary site of melanotic tumor formation during the third instar. We have functionally rescued these phenotypes with both genomic and cDNA transgenes. Importantly, the expression of a full-length krz cDNA within the CNS rescues the krz1 lethality. These experiments establish the krz nonvisual arrestin as an essential neural gene in Drosophila.
Laboratory and Field Evaluations of Food-Based Attractants for Monitoring German Cockroaches
Authors: Abbar Salehe , Wang Changlu 
Published: April 2021

AbstractSticky traps are standard tools for monitoring German cockroaches (Blattella germanica L.); however, because they lack an attractant, their ability to catch cockroaches is by chance and largely dependent upon the location of placement and length of time they are left in place. Currently, highly effective and economical cockroach attractants are not available. Food-based attractants have the potential to be employed in sticky traps and enhance their effectiveness on trapping German cockroaches. We conducted laboratory and field experiments to evaluate the effectiveness of novel food-based attractants. Laboratory results showed that sticky traps containing the following attractants: apple oil, blueberry oil, orange oil, or their combination, fish oil, peanut butter, Roach lure tablet, and bacon extract had significantly higher trap catch compared to those traps that do not contain an attractant. Apple plus blueberry oil mixture and Roach lure tablet enhanced the trap catch by ≥ 103%, and bacon extract enhanced the trap catch by 92% in the field assays. Apple plus blueberry oil mixture and Roach lure tablet were the most promising attractants based on laboratory and field experiments. Apple plus blueberry oil mixture aged for 2 wk was as attractive to German cockroaches as fresh mixture.
Victorian Faddishness: The Dolly Varden from Dickens to Patience
Authors: Mitchell Rebecca N 
Published: April 2021

Abstract
This article addresses the origins and nature of the fad through a case study of the ‘Dolly Varden’ dress. The gown originated in the 1770s as a ‘polonaise’ among continental aristocracy, and experienced a minor revival in the 1840s when Dolly Varden, a character in Charles Dickens’s novel Barnaby Rudge (1841) was depicted wearing it. But it did not become a fad until the 1870s, after a Dickens-owned painting of the character was sold and the gown re-entered the public eye. Having shed its historical or literary connections, the gown was revived again in Gilbert and Sullivan’s comic opera Patience (1881), a well-known send-up of Aesthetic foibles. Often overlooked is the fact that dairy maid Patience – who figures in the opera as a counterpoint to the lovesick maidens wearing high Aesthetic garb – wears a Dolly Varden gown, as faddish as any Aesthetic gown, only slightly outdated. The Aesthetic afterlife of the dress demonstrates the mutability of Victorian fads and the gleeful ignorance of antecedents that typifies fad culture. Facilitated by the material and commercial innovations of the period and fuelled by an insistent presentism, fads are – this article argues – a truly Victorian phenomenon.
Length variation in short tandem repeats affects gene expression in natural populations of Arabidopsis thaliana
Authors: Reinar William B. , Lalun Vilde O. , Reitan Trond , Jakobsen Kjetill S. , Butenko Melinka A. 
Published: April 2021

Abstract
The genetic basis for the fine-tuned regulation of gene expression is complex and ultimately influences the phenotype and thus the local adaptation of natural populations. Short tandem repeats (STRs) consisting of repetitive DNA motifs have been shown to regulate gene expression. STRs are variable in length within a population and serve as a heritable, but semi-reversible, reservoir of standing genetic variation. For sessile organisms, such as plants, STRs could be of major importance in fine-tuning gene expression as a response to a shifting local environment. Here, we used a transcriptome dataset from natural accessions of Arabidopsis thaliana to investigate population-wide gene expression patterns in light of genome-wide STR variation. We empirically modeled gene expression as a response to the STR length within and around the gene and demonstrated that an association between gene expression and STR length variation is unequivocally present in the sampled population. To support our model, we explored the promoter activity in a transcriptional regulator involved in root hair formation and provided experimentally determined causality between coding sequence length variation and promoter activity. Our results support a general link between gene expression variation and STR length variation in A. thaliana.
Future directions of the National Institutes of Health Science of Behavior Change Program
Authors: Keller Chandra , Ferrer Rebecca A , King Rosalind B , Collier Elaine 
Published: April 2021

Abstract

Background
The National Institutes of Health Science of Behavior Change Common Fund Program has accelerated the investigation of mechanisms of behavior change applicable to multiple health behaviors and outcomes and facilitated the use of the experimental medicine approach to behavior change research.

Purpose
This commentary provides a brief background of the program, plans for its next phase, and thoughts about how the experimental medicine approach to behavior change research can inform future directions in two areas of science—reproductive health and COVID-19 vaccine uptake.

Conclusions
The incorporation of a mechanisms-based approach into behavior intervention research offers new opportunities for improving health.
Integrative taxonomy reveals a new genus from Central Chile and suggests a systematic rearrangement in Stenochiinae (Coleoptera: Tenebrionidae)
Authors: Guerrero Marcelo , Vidal G-H Pedro , Zúñiga-Reinoso Álvaro 
Published: April 2021

AbstractStenochiinae is a subfamily of Tenebrionidae with a mainly pantropical distribution, but with a few species distributed at higher latitudes. In general, Stenochiinae has been little studied, and the taxonomic effort been insufficient to understand all its diversity. Recently, a new taxon of Stenochiinae from Central Chile with remarkable morphological characters was collected. Based on phylogenetic analyses of concatenated 28S, 18S, 16S and COI genes and on morphological analyses, a new genus and two species of Stenochiinae are described from Central Chile. Our results suggest that Nestorinus gen. nov. (with the species Nestorinus roitmani sp. nov. and Nestorinus yanettae sp. nov.) has a basal position in Stenochiinae, related to Homocyrtus. The set of morphological characters shared between Stenochiini and Cnodalonini plus their phylogenetic position make it difficult to classify them at tribal level.
Is more better? Do statewide increases in trauma centers reduce injury-related mortality?
Authors: Truong Evelyn I. , Ho Vanessa P. , Tseng Esther S. , Ngana Colette , Curtis Jacqueline , Curfman Eric T. , Claridge Jeffrey A. 
Published: April 2021

OBJECTIVES
Trauma centers are inconsistently distributed throughout the United States. It is unclear if new trauma centers improve care and decrease mortality. We tested the hypothesis that increases in trauma centers are associated with decreases in injury-related mortality (IRM) at the state level.

METHODS
We used data from the American Trauma Society to geolocate every state-designated or American College of Surgeons–verified trauma center in all 50 states and the District of Columbia from 2014 to 2018. These data were merged with publicly available IRM data from the Centers for Disease Control and Prevention. We used geographic information systems methods to map and study the relationships between trauma center locations and state-level IRM over time. Regression analysis, accounting for state-level fixed effects, was used to calculate the effect of total statewide number of trauma center on IRM and year-to-year changes in statewide trauma center with the IRM (shown as deaths per additional trauma center per 100,000 population, p value).

RESULTS
Nationwide between 2014 and 2018, the number of trauma center increased from 2,039 to 2,153. Injury-related mortality also increased over time. There was notable interstate variation, from 1 to 284 trauma centers. Four patterns in statewide trauma center changes emerged: static (12), increased (29), decreased (5), or variable (4). Of states with trauma center increases, 26 (90%) had increased IRM between 2014 and 2017, while the remaining 3 saw a decline. Regression analysis demonstrated that having more trauma centers in a state was associated with a significantly higher IRM rate (0.38, p = 0.03); adding new trauma centers was not associated with changes in IRM (0.02, p = 0.8).

CONCLUSION
Having more trauma centers and increasing the number of trauma center within a state are not associated with decreases in state-level IRM. In this case, more is not better. However, more work is needed to identify the optimal number and location of trauma centers to improve IRM.

LEVEL OF EVIDENCE
Epidemiologic, level III; Care management, level III.
Four-factor prothrombin complex concentrate in adjunct to whole blood in trauma-related hemorrhage: Does whole blood replace the need for factors?
Authors: Khurrum Muhammad , Ditillo Michael , Obaid Omar , Anand Tanya , Nelson Adam , Chehab Mohamad , Kitts Daniel James , Douglas Molly , Bible Letitia , Joseph Bellal 
Published: April 2021

BACKGROUND
The use of whole blood (WB) for the treatment of hemorrhagic shock and coagulopathy is increasing in civilian trauma patients. Four-factor prothrombin complex concentrate (4-PCC) in adjunct to component therapy showed improved outcomes in trauma patients. Our study aims to evaluate the outcomes of trauma patients who received 4-PCC and WB (4-PCC–WB) compared with WB alone.

METHODS
We performed a 3-year (2015–2017) analysis of the American College of Surgeons—Trauma Quality Improvement Program database. All adult (age, ≥18 years) trauma patients who received WB were included. We excluded patients who were on preinjury anticoagulants. Patients were stratified into two groups, 4-PCC–WB versus WB alone, and matched in a 1:2 ratio using propensity score matching. Outcome measures were packed red blood cells, plasma, platelets, and cryoprecipitate transfused, in-hospital complications, hospital and intensive care unit (ICU) length of stay (LOS) among survivors, and mortality.

RESULTS
A total of 252 patients (4-PCC–WB, 84; WB alone, 168) were matched. The mean ± SD age was 47 ± 21 years, 63% were males, median Injury Severity Score was 30 (21–40), and 87% had blunt injuries. Patients who received 4-PCC–WB had decreased requirement for packed red blood cell (8 U vs. 10 U, p = 0.04) and fresh frozen plasma (6 U vs. 8 U, p = 0.01) transfusion, lower rates of acute kidney injury (p = 0.03), and ICU LOS (5 days vs. 8 days, p = 0.01) compared with WB alone. There was no difference in the platelet transfusion (p = 0.19), cryoprecipitate transfusion (p = 0.37), hospital LOS (p = 0.72), and in-hospital mortality (p = 0.72) between the two groups.

CONCLUSION
Our study demonstrates that the use of 4-PCC as an adjunct to WB is associated with a reduction in transfusion requirements and ICU LOS compared with WB alone in the resuscitation of trauma patients. Further studies are required to evaluate the role of PCC with WB in the resuscitation of trauma patients.

LEVEL OF EVIDENCE
Therapeutic, level III.
Milestone Development in Genetic Conditions from SFARI Registries
Authors: Wickstrom Jordan , Farmer Cristan , Snyder LeeAnne Green , Mitz Andrew R. , Sanders Stephan J. , Bishop Somer , Thurm Audrey 
Published: April 2021

AbstractBackgroundSeveral recent initiatives have sought to better understand characteristic behaviors of rare genetic conditions associated with autism spectrum disorder (ASD). The onset of developmentally expected skills, such as walking and talking, serve as readily quantifiable aspects of the behavioral phenotype. The goals of this study were to describe attainment of major motor and language milestones in genetic conditions implicated in the etiology of ASD and other neurodevelopmental disorders and to compare those phenotypes to idiopathic ASD.MethodsParticipants ages 3 years and older were drawn from two Simons Foundation Autism Research Initiative-funded registries with consistent phenotyping protocols. Inclusion criteria consisted of a confirmed genetic diagnosis for one of 16 specific genetic conditions (Simons Searchlight), and absence of pathogenic genetic findings in idiopathic ASD controls (SPARK). Parent-reported age of acquisition of three motor and two language milestones was described and quantified as on-time or delayed relative to normative expectations.ResultsDelay was more common among participants with rare genetic conditions than idiopathic ASD for all milestones. Compared to the idiopathic ASD group, the median odds of delay among the genetic groups were 8.3 times (IQR 5.8-16.3) higher for sitting, 12.4 times (IQR 5.3-19.5) higher for crawling, 26.8 times (IQR 7.7-41.1) higher for walking, 2.7 times (IQR 1.7-5.5) higher for single words, and 5.7 times (IQR 2.8-18.3) higher for combined words.ConclusionDelays in major developmental milestones, particularly in motor skills, may be among the earliest clues that developmental processes may be differentially affected in specific genetically defined conditions versus a behaviorally defined disorder such as idiopathic ASD.
Cardiac Surgery during the Covid-19 Pandemic: Evidence from the first wave
Authors: Vandoros Sotiris , Schizas Nikolaos , Apostolopoulos Alkis , Patris Vasilios , Argiriou Mihalis 
Published: April 2021

AbstractBackgroundThe Covid-19 pandemic has affected human behaviour and burdened health systems and has thus had an impact on other health outcomes.ObjectiveThis paper studies whether there was a decrease in cardiac surgery operations in Greece during the first wave of the Covid-19 pandemic.Data and MethodsWe used data from 7 major hospitals that geographically cover about half the country and more than half the population, including a mix of public, private, military and children’s hospitals. We used a difference-in-differences econometric approach to compare trends in cardiac surgery before and after the pandemic in 2020, to the same months in 2019, controlling for seasonality and unemployment, and using hospital fixed effects.ResultsWe found that during the first wave of the pandemic and the associated lockdown, there were 35-56% fewer cardiac surgery operations compared to what we would have expected in the absence of the pandemic.ConclusionsThere was a steep decline in Cardiac surgery operations in Greece during the first wave of the Covid-19 pandemic. Possible reasons may include people not seeking medical attention to avoid the risk of catching Covid-19; fewer referrals; and working from home, thus not being exposed to a stressful work environment or commute.
Hematopoietic stem cell requirement for macrophage regeneration is tissue-specific
Authors: Eddins Devon J. , Kosters Astrid , Waters Jeffrey , Sosa Jasmine , Phillips Megan , Yadava Koshika , Herzenberg Leonore A. , Kuipers Hedwich F. , Ghosn Eliver Eid Bou 
Published: April 2021

AbstractTissue-resident macrophages (TRMΦ) are important immune sentinels responsible for maintaining tissue and immune homeostasis within their specific niche. Recently, the origins of TRMΦ have undergone intense scrutiny where now most TRMΦ are thought to originate early during embryonic development independent of hematopoietic stem cells (HSCs). We previously characterized two distinct subsets of mouse peritoneal cavity macrophages (Large and Small Peritoneal Macrophages; LPM and SPM, respectively) whose origins and relationship to both fetal and adult long-term (LT)-HSCs have not been fully investigated. Here we employ highly purified LT-HSC transplantation and in vivo lineage tracing to show a dual ontogeny for LPM and SPM, where the initial wave of peritoneal macrophages is seeded from yolk sac-derived precursors, which later require LT-HSCs for regeneration. In contrast, transplanted fetal and adult LT-HSCs are not able to regenerate brain-resident microglia. Thus, we demonstrate that LT-HSCs retain the potential to develop into TRMΦ, but their requirement is tissue-specific.
Retinal ganglion cell survival after severe optic nerve injury is modulated by crosstalk between JAK/STAT signaling and innate immune responses in the zebrafish retina
Authors: Chen Si , Lathrop Kira L. , Kuwajima Takaaki , Gross Jeffrey M. 
Published: April 2021

ABSTRACTVisual information is transmitted from the eye to the brain along the optic nerve, a structure composed of retinal ganglion cell (RGC) axons. The optic nerve is highly vulnerable to damage in neurodegenerative diseases like glaucoma and there are currently no FDA-approved drugs or therapies to protect RGCs from death. Zebrafish possess remarkable neuroprotective and regenerative abilities and here, utilizing an optic nerve transection (ONT) injury and an RNA-seq-based approach, we identify genes and pathways active in RGCs that may modulate their survival. Through pharmacological perturbation, we demonstrate that JAK/STAT pathway activity is required for RGC survival after ONT. Furthermore, we show that immune responses directly contribute to RGC death after ONT; macrophages/microglia are recruited to the retina and blocking neuroinflammation or depleting these cells after ONT rescues survival of RGCs. Taken together, our results support a model in which pro-survival signals in RGCs, mediated by JAK/STAT signaling, counteract the activity of innate immune responses to modulate RGC vulnerability and resilience in the zebrafish retina after severe optic nerve damage.
Differential Interaction between RAC/ROP-GTPases and RIC-Effectors: A Network Hub for Broader Signal Transduction in Pollen Tubes
Authors: Stephan Octavian O. H. 
Published: April 2021

ABSTRACTTo date knowledge about plant RAC/ROP-GTPase effectors and downstream targets is still limited. This work aims on elucidation of related signal transduction networks involved in pollen tube growth. Yeast two-hybrid and Pull Down methodology were used to identify and characterize hitherto unknown components of RAC-related protein complexes fromNicotiana tabacum. Nt-RIC11pt specifically interacts with diverse active tobacco RAC-GTPases, and it is particularly significant, that their binding affinity is differential, thus implicating a multifaceted role in an interconnected RIC-RAC network. Moreover, Y2H-screening for Nt-RIC11pt targets identified Nt-CAR4, which is phylogenetically assigned to a multifaceted family of novel unusualGTPaseactivatingproteins (GAP). It is argued that scaffold Nt-RIC11pt connects active Nt-RAC3 with membrane-bound Nt-CAR4, thus relaying GAP-activity. Quantitative RT-PCR demonstrates Nt-RIC11pt is primarily expressed in pollen and YFP-fusion proteins show homogeneous cytoplasmic localization in growing tubes, what builds the prerequisite for a proposed role in broader signal transduction. By synoptically integrating experimental data, bioinformatic sequence comparison, phylogenetic analyses, and detailed literature review, this study hypothesizes a concept in which RIC-effectors collectively constitute a multifaceted network hub linking diverse GTPase-dependent processes.