bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D Chromatin Remodeling Potentiates Transcriptional Programs Driving Cell Invasion
Authors: Lebeau Benjamin , Jangal Maika , Zhao Tiejun , Wong Cheng Kit , Wong Nolan , Canedo Eduardo Cepeda , Hebert Steven , Aguilar-Mahecha Adriana , Chabot Catherine , Buchanan Marguerite , Catterall Rachel , McCaffrey Luke , Deblois Genevieve , Kleiman Claudia , Park Morag , Basik Mark , Witcher Michael 
Published: October 2021

Abstract
The contribution of deregulated chromatin architecture, including topologically associated domains (TADS), to cancer progression remains ambiguous. CTCF is a central regulator of higher-order chromatin structure that undergoes copy number loss in over half of all breast cancers, but the impact of this defect on epigenetic programming and chromatin architecture remains unclear. We find that under physiological conditions, CTCF organizes sub-TADs to limit the expression of oncogenic pathways, including PI3K and cell adhesion networks. Loss of a single CTCF allele potentiates cell invasion through compromised chromatin insulation and a reorganization of chromatin architecture and histone programming that facilitates de novo promoter-enhancer contacts. However, this change in the higher-order chromatin landscape leads to a vulnerability to inhibitors of mTOR. These data support a model whereby sub-TAD reorganization drives both the modification of histones at de novo enhancer promoter-contacts and transcriptional upregulation of oncogenic transcriptional networks.
Effect of Electron-Withdrawing Fluorine and Cyano Substituents on Photovoltaic Properties of Two-Dimensional Quinoxaline-Based Polymers
Authors: Lee Seok Woo , Hussain MD Waseem , Shome Sanchari , Ha Su Ryong , Oh Jae Taek , Whang Dong Ryeol , Kim Yunseul , Kim Dong-Yu , Choi Hyosung , Chang Dong Wook 
Published: October 2021

Abstract
In this study, strong electron-withdrawing fluorine (F) and cyano (CN) substituents are selectively incorporated into the quinoxaline unit of two-dimensional (2D) D–A-type polymers to investigate their effects on the photovoltaic properties of the polymers. To construct the 2D polymeric structure, electron-donating benzodithiophene and methoxy-substituted triphenylamine are directly linked to the horizontal and vertical directions of the quinoxaline acceptor, respectively. After analyzing the structural, optical, and electrochemical properties of the resultant F- and CN-substituted polymers, labeled as PBCl-MTQF and PBCl-MTQCN, respectively, inverted-type polymer solar cells with a non-fullerene Y6 acceptor are fabricated to investigate the photovoltaic performances of the polymers. It is discovered that the maximum power conversion efficiency of PBCl-MTQF is 7.48%, whereas that of PBCl-MTQCN is limited to 3.10%. This significantly reduced PCE of the device based on PBCl-MTQCN is ascribed to the formation of irregular, large aggregates in the active layer, which can readily aggravate the charge recombination and charge transport kinetics of the device. Therefore, the photovoltaic performance of 2D quinoxaline-based D–A-type polymers is significantly affected by the type of electron-withdrawing substituent.
Prevalence and Antimicrobial Susceptibility Pattern of Urinary Tract Infection among Pregnant Women Attending Hargeisa Group Hospital, Hargeisa, Somaliland
Authors: Ali Abdikhaliq Hussein , Reda Dawit Yihdego , Ormago Moges Desta 
Published: October 2021

Abstract
The aim of this study was to determine the prevalence, antimicrobial susceptibility pattern and associated factors of urinary tract infection (UTI) among pregnant women attending Hargeisa Group Hospital (HGH), Hargeisa, Somaliland. A cross-sectional study was conducted at HGH, Hargeisa, Somaliland and participants were selected by systematic random sampling technique. Clean catch midstream urine samples were collected from 422 participants and cultured and antimicrobial susceptibility pattern was determined for the isolates. Univariable and multivariable logistic regression analyses were utilized to identify the independent risk factors for UTI The prevalence of UTI was 16.4 % (95% CI: 13.3–19.9). The predominant bacteria isolate was E. coli (43.5%). Gram negative bacteria were resistant to ampicillin (96%) and tetracycline (71.4%) and Gram-positive bacteria were also resistant to ampicillin (90%), tetracycline (55%). Multidrug resistance was observed in 89.9% of bacterial isolated. No formal education participants were 3.18 times, previous history of catheterization had 3.22 times and previous history of UTI had 3.73 times more likely to develop UTI than their counterparts respectively. Culture and susceptibility test is vital for appropriate management of UTI in the study area.
Effective Biomarkers and Therapeutic Targets of Nerve-Immunity Interaction in The Treatment of Depression: A Integrated Analysis of miRNA-mRNA Regulatory Networks
Authors: Wu Zi-xuan , Huang Xuyan , Cai Min-jie , Huang Peidong , Guan Zunhui 
Published: October 2021

Abstract
Background: Major depressive disorder (MDD) is an emotional disorder that has a negative effect on patients' studies and daily lives. A great number of studies have found that miRNAs play an important role in the development of MDD and that they can be used as a biomarker for the diagnosis and treatment of MDD. However, there have been few investigations on nerve-immunity interaction therapy for MMD patients' brains.Methods: We attempted to evaluate MDD in the gene expression matrix database and miRNAs in plasma samples from healthy controls using bioinformatics methods. Four plasma miRNAs (DE-miRNAs) samples were found from MDD patients. Funrich planned the transcription factors and target genes of miRNAs, and the enrichment of TF and GO was examined. The intersecting mRNAs were discovered by comparing the various expressions of the projected target genes and 5 mRNAs (DE-mRNAs) samples. In the end, 34 DE-miRNAs, 386 DE-mRNAs, and 17 intersecting mRNAs were detected. Intersecting core genes were then investigated using GO and KEGG enrichment analysis to find the intersecting mRNA. Identify particular candidate genes and pathways in neurology and immunology that may be associated with MDD for further investigation.Results: We discovered 17 important HUB genes by the advance of a miRNA-mRNA network, and 5 HUB DE-MRNAs were derived following CytoNCA topology.Conclusion: Our findings from a comprehensive bioinformatics analysis of miRNAs and mRNAs in MDD show that DE-miRNAs like miR-338-3P and miR-206 may be excellent biomarkers and potential therapeutic targets for the treatment of MDD via nerve-immunity interaction.
Perioperative Supplemental Oxygen and Plasma Catecholamine Concentrations After Major Abdominal Surgery – a Substudy of a Randomized Clinical Trial
Authors: Taschner Alexander , Kabon Barbara , von Sonnenburg Markus Falkner , Graf Alexandra , Adamowitsch Nikolas , Fraunschiel Melanie , Fleischmann Edith , Reiterer Christian 
Published: October 2021

Abstract
BackgroundIncreased sympathetic nerve activity due to perioperative stress is associated with higher plasma catecholamine concentrations that lead to an increase in heart rate and blood pressure. This fact plays a pivotal role in the development of perioperative myocardial ischemia. A previous study in healthy volunteers has shown that the administration of supplemental oxygen attenuated sympathetic nerve activity, which was associated with lower plasma catecholamine concentrations. However, in patients undergoing surgery evidence is still lacking. We therefore tested the hypothesis that perioperative supplemental oxygen attenuates sympathetic nerve activity estimated by plasma catecholamines in patients at risk for cardiovascular complications undergoing major abdominal surgery.MethodsWe randomly assigned 81 patients to receive either 80% versus 30% inspired oxygen concentration throughout surgery and for the first two postoperative hours. We assessed noradrenaline, adrenaline and dopamine plasma concentrations as surrogate parameters for sympathetic nerve activity before induction of anesthesia, two hours after surgery and on the third postoperative day.Results41 patients received 80% oxygen and 40 patients received 30% oxygen. There was no significant difference in postoperative noradrenaline (effect estimated:-41.5 ng.L-1, 95%CI -134.3, 51.2; p=0.38), adrenaline (effect estimated:11.2 ng.L-1, 95%CI -7.6, 30.1; p=0.24) and dopamine (effect estimated:-1.61 ng.L-1, 95%CI -7.2, 3.9; p=0.57) concentrations between both groups. ConclusionsWe found no significant difference in postoperative plasma catecholamine concentration between the 80% and 30% oxygen group in patients at risk for cardiovascular complications undergoing major abdominal surgery. Based on our results, it seems likely that supplemental oxygen did not influence sympathetic nerve activity in the perioperative setting.Trial RegistrationClinicalTrials.gov (NCT 03366857)European Clinical Trial Database (EudraCT 2017-003714-68)
The Application of Different Breathing Training Methods in Elderly Patients With Femoral Neck Fracture: A Prospective, Randomized, and Single-Blind Study
Authors: Zhang Yan , Wang Hong , Fan Mei Jing , Fan Lin , Wang Min 
Published: October 2021

Abstract
Purpose: Femoral neck fractures in elderly patients lead to reduced mobility and increased risk of pulmonary complications. The main purpose of this study is to observe the forced expiratory volume in the first second(FEV1), forced vital capacity(FVC), blood oxygen saturation(SPO2), pulmonary complications, and hospitalization days in elderly patients with femoral neck fractures, and to evaluate the use of lung function trainers to train elderly patients’ lung function effect and feasibility.Methods: This is a prospective, randomized and single-blind study. 108 elderly patients with femoral neck fractures over 70 years old were randomly divided into a conventional group and a lung function trainer group. The conventional group received effective cough training and lip-reducing breathing training, and the lung function trainer group received effective cough training and lung function training. Until 2 weeks after surgery. The main outcomes are forced expiratory volume in the first second, forced vital capacity, and blood oxygen saturation. The secondary outcomes are pulmonary complications and the hospitalization days.Results: 50 subjects in the conventional group and 51 subjects in the lung function trainer group completed the study. On the 3rd and 7th day of training, the FVC of the lung function trainer group was higher than that of the conventional group (2.94±0.40vs.2.72±0.34,P<0.05；3.02±0.40vs.2.86±0.34,P<0.05), and the FVE1 of the the lung function trainer group was higher than that of the conventional group (1.99±0.37vs.1.80±0.42，P＜0.05；2.14±0.39vs.1.94±0.43,＜0.05), and the blood oxygen saturation of the lung function trainer group was higher than that of the conventional group ( 97.10±1.35vs.96.28±1.21；97.47±1.35vs.96.86±1.36,P＜0.05), 4 cases of pneumonia in the conventional group, no one in the lung function trainer group was diagnosed with pneumonia, and the hospitalization days in the lung function trainer group was less than that in the conventional group (15.55±1.45VS.17.2±2.58,P<0.05).Conclusion: For elderly patients with femoral neck fracture, the use of lung function training device to strengthen respiratory muscle function training during perioperative period can improve the patient's lung function in a short period of time, reduce the complications of respiratory system infection, and shorten the hospitalization days. It is worthy of clinical application.
The single-cell transcriptional landscape of the axolotl
Authors: Ye Fang , Zhang Guodong , E Weigao , Chen Haide , Yu Chengxuan , Li Jiaqi , Fu Yuting , Sun Zhongyi , Fei Lijiang , Guo Qile , Yang Lei , Wang Jingjing , Xiao Yanyu , Wang Xinru , Zhang Peijing , Ma Li Feng , Fu Sulei , Fei Jifeng , Ge Dapeng , Xu Suhong , Han Xiaoping , Guo Guoji 
Published: October 2021

Abstract
The Mexican axolotl (Ambystoma mexicanum) is a promising tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, during which their regeneration capacity and lifespan gradually decline. However, a system-level single-cell analysis of molecular characteristics in neotenic and metamorphosed axolotls is still lacking. Here, we developed a single-cell RNA-seq method based on combinatorial hybridization to generate a tissue-based transcriptomic atlas of the adult axolotl. We performed gene expression profiling of over 1 million single cells across 19 tissues to construct the first adult axolotl cell atlas. Comparison of single-cell transcriptomes between the tissues of neotenic and metamorphosed axolotls revealed the heterogeneity of structural cells in different tissues and established their regulatory network. Furthermore, we described dynamic gene expression patterns during limb development in neotenic axolotls. These data serve as a resource to explore the molecular identity of the axolotl as well as its metamorphosis.
­TREM2-H157Y Increases Soluble TREM2 Production and Reduces Amyloid Pathology
Authors: Qiao Wenhui , Chen Yixing , Martens Yuka A. , Liu Chia-Chen , Knight Joshua A. , Li Fuyao , Chen Kai , Aishe Kurti I. , Shue Francis , Dacquel Maxwell V. , Fryer John , Zhao Na , Bu Guojun 
Published: October 2021

Abstract
Background: The p.H157Y variant of TREM2 (Triggering Receptor Expressed on Myeloid Cells 2) has been reported to increase Alzheimer’s disease (AD) risk. This mutation in the extracellular domain of TREM2 localizes at the cleavage site and was shown to enhance shedding and impair phagocytosis in vitro by ectopic expression of TREM2-H157Y in HEK293 cells. However, the physiological and AD-related outcomes of TREM2 H157Y mutation in vivo remain unknown.Methods: We generated a novel Trem2 H157Y knock-in mouse model through CRISPR-Cas9 technology and investigated how Trem2 H157Y mutation impacts TREM2 proteolytic processing, synaptic function, and AD-related amyloid pathology by conducting biochemical assays, immunofluorescent staining, hippocampal electrophysiology, and in vivo micro-dialysis in awake, free-moving animals.Results: Consistent with previous in vitro findings, TREM2-H157Y increases the amount of soluble TREM2 (sTREM2) in the cortex and serum of mutant mice compared to the wild type controls. Interestingly, the Trem2 H157Y variant enhances synaptic plasticity without affecting microglial density and morphology. In the presence of amyloid pathology, TREM2-H157Y surprisingly accelerates Aβ clearance and reduces amyloid burden and microgliosis. Conclusion: Taken together, our findings support a beneficial effect of the Trem2 H157Y mutation in synaptic function and in mitigating amyloid pathology. Considering the genetic association of TREM2 p.H157Y with AD, we speculate TREM2-H157Y might increase AD risk through an amyloid-independent pathway, as such its effects on tauopathy and neurodegeneration merit further investigation.
Retrospective Analysis of Risk Factors for Postoperative Perineal Hernia After Endoscopic Abdominoperineal Excision for Rectal Cancer
Authors: Manabe Tatsuya , Mizuuchi Yusuke , Tsuru Yasuhiro , Kitagawa Hiroshi , Fujimoto Takaaki , Koga Yasuo , Nakamura Masafumi , Noshiro Hirokazu 
Published: October 2021

Abstract
Background: In contrast to open-surgery abdominoperineal excision (APE) for rectal cancer, postoperative perineal hernia (PPH) is reported to increase after extralevator APE and endoscopic surgery. In this study, therefore, we aimed to determine the risk factors for PPH after endoscopic APE.Methods: A total 73 patients who underwent endoscopic APE for lower rectal cancer were collected from January 2009 to March 2020, and the risk factors for PPH were analyzed retrospectively.Results: Nineteen patients (26%) developed PPH after endoscopic APE, and the diagnosis of PPH was made at 9–393 days (median: 183 days) after initial surgery. Logistic regression analysis showed that absence of pelvic peritoneal closure alone increased the incidence of PPH significantly (odds ratio; 13.76, 95% confidence interval; 1.48–1884.84, p = 0.004).Conclusions: Pelvic peritoneal closure should be performed when possible after endoscopic APE to prevent PPH.
Donor-Acceptor Pair Quantum Emitters in Hexagonal Boron Nitride
Authors: Tan Qinghai , Lai Jia-Min , Liu Xue-Lu , Guo Dan , Xue Yong-Zhou , Dou Xiu-Ming , Sun Bao-Quan , Deng Hui-Xiong , Tan Ping-Heng , Aharonovich Igor , Gao Weibo , Zhang Jun 
Published: October 2021

Abstract
Quantum emitters are needed for a myriad of applications ranging from quantum sensing to quantum computing. Hexagonal boron nitride (hBN) quantum emitters are the most promising solid-state platform to date due to its high brightness, stability, and the possibility of spin photon interface. However, the understanding of the physical origins of the single-photon emitters (SPEs) is still limited. Here, we present concrete and conclusive evidence that the dense SPEs in hBN, across entire visible spectrum, can be well explained by donor-acceptor pairs (DAPs). Based on the DAP transition generation mechanism, we have calculated their wavelength fingerprint, matching well with the experimentally observed photoluminescence spectrum. Our work serves as a step forward for the physical understanding of SPEs in hBN and their applications in quantum technologies.
Petrology of the opening eruptive phase of the 2021 Cumbre Vieja eruption, La Palma, Canary Islands
Authors: Pankhurst Matthew J. , Scarrow Jane H. , Barbee Olivia A. , Hickey James , Coldwell Beverley C. , Rollinson Gavyn , Rodriguez-Losada Jose A. , Martín-Lorenzo Alba , Rodríguez Fátima , Hernández William , Hernández Pedro A. , Pérez Nemesio M. 
Published: October 2021

Abstract
The first products of the current Cumbre Vieja eruption comprise simultaneous tephra fall from near-continuous, gas-rich eruption plumes and lava flows. From combined field, petrographic and geochemical analyses we identify: low percentage mantle melts with a variably-equilibrated multimineralic crystal-cargo and compositional fractionation by eruptive processes. Hence petrology can untangle complex magmatic and volcanic processes for this eruption, which through further study can assist in active decision making.
Influence of Pregnancy on Neuromyelitis Optica From an Ophthalmological Point of View: a Case Report and Literature Review
Authors: Aslan Fatih , Tuğba-Çimşir Meral , Özdemir-Öktem Ece 
Published: October 2021

Abstract
Neuromyelitis Optica (NMO, Devic’s disease) is a rare demyelinating disease of the central nervous system leading to optic neuritis and transverse myelitis. The clinical course of the disease and ocular changes in pregnancy is still not well known. Here, we present the subclinical ophthalmological changes which are evaluated by spectral domain optical coherence tomography (SD-OCT) during the 39 weeks gestation and postpartum period in a pregnant woman with a diagnosis of NMO. Additionally, we present the obstetric and neurologic course of our patient and review the literature. A 30-year-old female with a history of NMO had been ophthalmologically examined and performed SD-OCT every trimester periodically to observe the effects of pregnancy on the disease course. No ophthalmological changes were observed during the pregnancy and postpartum period. Cesarean delivery was preferred due to obstetric indication. The patient was discharged on the second day of postpartum period.
Formula Feeding and Associated Factors among Mothers with Infants 0-6 Months Old in Mettu Town, South West Ethiopia
Authors: Mitiku Abeza 
Published: October 2021

Abstract
Background

Adequate nutrition during infancy is essential for the normal development and future wellbeing of the child. Despite the fact that breast milk is optimal nutrition for infants during the first six months of life, the duration of breastfeeding has been declining and being replaced by formula feeding particularly in urban communities of developing countries including Ethiopia. However, relatively little information is available regarding formula feeding in Ethiopia; particularly in Mettu town.
Methods

Community-based cross-sectional study was conducted in Mettu town from May, 17 to July 1, 2021, among 366 mothers with infants 0-6 months old. A simple random sampling technique was employed. Pre-tested semi-structured questionnaires were used to collect data. Descriptive statics and multivariable logistic regression were performed, and variables with a p-value <0.05 in the final model were declared statistically significant with formula feeding.
Result

The prevalence of formula feeding among the study participants was 28.4%, 95% [CI: 24.0-33.0]. In multivariable logistic regression analysis primparity [AOR=3.3, 95% CI:(1.71-6.26)], cesarean delivery [AOR=2.6, 95% CI:(1.28-5.35)], initiation of breastfeeding after 24hrs [AOR=3.7,95% CI:(1.24-10.05)] employed mothers [AOR=2.4, 95% CI:(1.29-4.49] poor knowledge [AOR=2.6, 95%CI:(1.49-4.73)] and positive attitude toward formula feeding [AOR= 2.4, 95% CI: (1.27-4.84)] were significantly associated with formula feeding.
Conclusion

About one-third of mothers were formula feeding their infant. Primi parity, initiation of breast milk after 24hrs, cesarean delivery, and poor maternal knowledge and positive attitude were significantly associated with formula feeding. Hence, education on infant feeding for pregnant and lactating mothers, and supporting primipara mothers and as well as mothers who deliver by cesarean section should be implemented at health facility and community level.
LA FORMACIÓN DE LOS EDUCADORES MUSICALES EN MÉXICO: RETOS Y PROPUESTAS
Authors: Pliego Carrasco Gabriel 
Published: October 2021

En México, las reformas curriculares vigentes para la educación musical en los niveles básicos obligatorios, presentan dilemas que desfavorecen la consecución de los propósitos planteados por ellas mismas. Las principales razones son tres: los programas de educación musical no están realizados sobre un sistema de pensamiento o análisis profundo de la naturaleza de la música y su educación; en estas reformas no se precisaelperfil deseado para el educador musical, y existe una limitada cantidad de opciones para la formación profesional del educador musical.
ad de los programas actuales de educación musical, y una gama de opciones serias de formación universitaria del profesorado de educación musical, en ámbitos de creación musical y prácticas didácticas reales. El objetivo de esta investigación es considerar a la filosofía praxial de la educación musical, junto con el análisis del programa universitario de educación musical de la Escuela Nacional de Música (ENM) de la Universidad Nacional Autónoma de México (UNAM), como fundamento que ofrece bases y lineamientos para el desarrollo de un currículo universitario de educación musical en la Ciudad de México. Estos fundamentos definen el perfil de un educador musical y brindan, a su vez, los pilares para el desarrollo de los programas de música para la educación básica en México.