bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lanthanide Tag in Search For Paramagnetically Induced Residual Dipolar Couplings in Small Molecules
Authors: Yassin Ali , Kantar Sally El , Obeid Sandy , Akoury Elias 
Published: October 2021

Abstract
The structure elucidation of molecules and determination of their characteristic interactions and thermodynamic processes have become routine measurements while using Nuclear Magnetic Resonance (NMR) spectroscopy as an indispensable technique. The theoretical description of NMR spectroscopy and treatment of the dynamics of nuclear spin systems is highly dependent on quantum mechanics. More precisely, Residual Dipolar Coupling (RDC) provides global orientation information of magnetic dipole-dipole interaction in a reference frame and distance-independent angular insights to local relaxation. RDC measurements necessitates the use of an anisotropic orientation medium for the partial alignment of the target molecule with respect to the magnetic field. Such media include liquid crystals or paramagnetic centers with a lanthanide ion. Anisotropic paramagnetic tags have been successful in biomolecular NMR applications of large proteins but their use in small organic molecules remains imperfect due to challenges in designing functional lanthanide-complexes with varying degrees of nondirectional bonding in the Ln(III) inner coordination sphere. In this study, we propose a strategy for the synthesis of a lanthanide tag and the measurement of RDCs in a target organic molecule using several paramagnetic lanthanide complexes.
Activation of Gαq sequesters specific transcripts into Ago2 particles
Authors: Jackson Lela , Poussaint Alison , Scarlata Suzanne 
Published: October 2021

Abstract
Hormones and neurotransmitters can activate the Gαq / phospholipase Cβ1 (PLCβ1) signaling system eliciting cellular calcium responses. PLCβ1 also prevents the aggregation of ribosomal and RNA proteins into stress granules, which are halted translation complexes that form in response to cellular stress. Activation of Gαq promotes PLCβ1association releasing bound proteins and promoting the formation of stress granules. However, the cellular impact of stress granules formed from routine Gαq protein signaling is unknown. Here, we have characterized Ago2 stress granules formed in response to Gαq activation in a neuronal-like cell line. We find these stress granule have a distinct protein composition, and unlike stress granules formed under heat stress, contain only two mRNA transcripts, chromogranin B, which is involved in secretory function, and ATP synthase 5f1b, which is required for ATP synthesis. Our studies show an unexpected pathway where Gαq/PLCβ regulates the translation of specific proteins.
Weight-Loss Behaviors of Avoiding Between Meal and Midnight Snack in Teenagers Associated with Gestational Diabetes: The Japan Environment and Children’s Study
Authors: Minami Marina , Watanabe Takafumi , Eitoku Masamitsu , Maeda Nagamasa , Fujieda Mikiya , Suganuma Narufumi 
Published: October 2021

Abstract
Background: Dietary habits and weight control before pregnancy are important in preventing gestational diabetes. This study aims to examine whether the weight-loss behavior of avoiding between meal and midnight snacks in teenagers is associated with subsequent gestational diabetes mellitus (GDM).Methods: A total of 89,227 (85.7% of the total) mother-infant pairs of live births were included in our study of the Japan Environment and Children's Study (JECS). In the second or third trimesters, participants were asked to report their weight-loss behaviors during their teenage years. Response items included avoidance of meals and midnight snacking. The main outcome of our study was the prevalence of GDM.Results: Overall, 2,066 (2.3%) participants had GDM. Relative to those without GDM, women with GDM were older, were smokers, had a higher prevalence of hypertension, previous cesarean delivery, mental illness, and higher body mass index (BMI). Weight-loss behavior in their teenage years was associated with a decreased risk of GDM (unadjusted crude odds ratio, 0.83; 95% confidence interval [CI]: 0.76–0.91), model 1 (adjusted odds ratio [aOR], 0.86; 95% CI: 0.79–0.94), and model 2 (aOR, 0.80; 95% CI: 0.73–0.88). Weight-loss behavior in teens was associated with a decreased risk of GDM in the normal weight [aOR, 0.79; 95% CI: 0.70–0.89) and overweight (aOR, 0.82; 95% CI: 0.69–0.98) groups.Conclusions: The results suggest that weight-loss behaviors of avoiding in-between meals and midnight snacking as teenagers are associated with a decreased risk of developing GDM.
DAXX-ATRX-H3.3 Regulation of p53 Chromatin Binding and DNA Damage Response
Authors: Gulve Nitish , Deng Zhong , Soldan Samantha , Vladimirova Olga , Wickramasinghe Jayamanna , Zheng Hongwu , Kossenkov Andrew , Lieberman Paul 
Published: October 2021

Abstract
DAXX and ATRX are tumor suppressor proteins that form a histone H3.3 chaperone complex and are frequently mutated in cancers with the alternative lengthening of telomeres (ALT), such as pediatric glioblastoma. Rapid loss of function of either DAXX or ATRX are not by themselves sufficient to induce the ALT phenotype. However, cells lacking DAXX or ATRX can be readily selected for ALT-like features. Here, we show that DAXX and ATRX null glioblastoma cells with ALT-like features have defects in p53 chromatin binding and DNA damage response regulation. RNA-seq analysis of DAXX or ATRX null U87 glioblastoma cells with ALT-like features revealed that p53 pathway is among perturbed. ALT-selected DAXX and ATRX-null cells had aberrant response to DNA damaging agent etoposide. Both DAXX and ATRX-null ALT cells showed a loss of p53 binding at a subset of response elements. Complementation of DAXX null cells with a wild-type DAXX transgene rescued p53 binding and transcription, while the tumor associated mutation L130R that disrupts ATRX binding was incapable of rescuing p53 chromatin binding. We show that histone H3.3 binding is reduced in DAXX-null cells especially at subtelomeric p53 binding sites and telomere repeats. These findings indicate that DAXX and ATRX function to enable p53 chromatin binding through modulation of histone H3.3 binding, especially at sub-telomeric sites.
A phylogenetically-restricted essential cell cycle progression factor in the human pathogen Candida albicans
Authors: Jaitly Priya , Legrand Melanie , Das Abhijit , Patel Tejas , Chauvel Murielle , d'Enfert Christophe , Sanyal Kaustuv 
Published: October 2021

Abstract
Chromosomal instability in fungal pathogens caused by cell division errors is associated with antifungal drug resistance. To identify mechanisms underlying such instability and to uncover new potential antifungal targets, we conducted an overexpression screen monitoring chromosomal stability in the human fungal pathogen Candida albicans. Analysis of ~1000 genes uncovered six chromosomal stability (CSA) genes, five of which are related to cell division genes in other organisms. The sixth gene, CSA6, is selectively present in the CUG-Ser clade species that includes C. albicans and other human fungal pathogens. The protein encoded by CSA6 localizes to the spindle pole bodies, is required for exit from mitosis, and induces a checkpoint-dependent metaphase arrest upon overexpression. Together, Csa6 defines an essential CUG-Ser fungal clade-specific cell cycle progression factor, highlighting the existence of phylogenetically-restricted cell division genes which may serve as potential unique therapeutic targets.
Protein Partitioning In A Droplet-Based Aqueous-Two Phase System Microfluidic Device
Authors: Cid David Hernández , Gallo-Villanueva Roberto Carlos , González-Valdez José , González Victor Hugo Pérez , Mata-Gómez Marco Arnulfo 
Published: October 2021

Abstract
Aqueous-Two Phase Systems (ATPS) is an important tool for the separation of biological entities as proteins, membranes, enzymes, among others. On the other hand, microfluidics is an emerging technology that studies and manipulates liquids either one single phase or dispersed fluids such as droplets at the micro or smaller scales. Applications of microfluidics in different areas such as molecular biology, biochemical analysis and bioprocess have increased in the last years. In this work, we proposed a droplet-based microfluidic approach to generate ATPS systems and to observe how two model proteins, native ribonuclease A (RNase A) and its PEGylated form (PEG-RNase A), behave and partition on these systems. Using polyethylenglycol (PEG) and potasium phosphate salts as the phase-forming chemicals, we were able to form ATPS systems inside the microfluidic device as commonly performed in conventional ATPS macrosystems. Even more, formation of ATPS systems in which one of the fluids was present as a droplet was also achieved. As expected, model proteins exhibited the same behavior as they do in a macrosystem, that is, they displaced to a particular phase according to their affinity for them. When native RNase A was placed in the salt-rich phase, it remained there, and migrated from the PEG-rich phase to the former. On its part, PEGylated RNase A remained in the PEGrich phase or migrated from salt-rich phase to the PEG-rich phase. These results open the possibility for a prospect of micro bioprocess to separate interest biomolecules.
The differential diagnosis of IgG4-related disease based on machine learning
Authors: Yamamoto Motohisa , Nojima Masanori , Kamekura Ryuta , Kuribara-Souta Akiko , Uehara Masaaki , Yamazaki Hiroki , Yoshikawa Noritada , Aochi Satsuki , Mizushima Ichiro , Watanabe Takayuki , Nishiwaki Aya , Komai Toshihiko , Shoda Hirofumi , Kitagori Koji , Yoshifuji Hajime , Hamano Hideaki , Kawano Mitsuhiro , Takano Ken-ichi , Fujio Keishi , Tanaka Hirotoshi 
Published: October 2021

Abstract
Introduction:
To eliminate the disparity and maldistribution of physicians and medical specialty services, the development of diagnostic support for rare diseases using artificial intelligence is being promoted. Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a rare disorder often requiring special knowledge and experience to diagnose. In this study, we investigated the possibility of differential diagnosis of IgG4-RD based on basic patient characteristics and blood test findings using machine learning.
Methods
Six-hundred and two patients with IgG4-RD and 212 patients with non-IgG4-RD that needed to be differentiated who visited the participating institutions were included in the study. Ten percent of the subjects were randomly excluded as a validation sample. Among the remaining cases, 80% were used as training samples, and the remaining 20% were used as test samples. Finally, validation was performed on the validation sample. The analysis was performed using a decision tree and a random forest model. Furthermore, a comparison was made between conditions with and without the serum IgG4 concentration. Accuracy was evaluated using the area under the receiver-operating characteristic (AUROC) curve.
Results
In diagnosing IgG4-RD, AUROC curve values of the decision tree and the random forest method were 0.905 and 0.970, respectively, when serum IgG4 levels were included in the analysis. Excluding serum IgG4 levels, the AUROC curve value of the analysis by the random forest method was 0.919.
Conclusion
Based on machine learning in a multicenter collaboration, with or without serum IgG4 data, basic patient characteristics and blood test findings alone were sufficient to differentiate IgG4-RD from non-IgG4-RD.
Clinical presentations and surgical outcomes of hemifacial spasm involving the vertebral artery
Authors: Li Jianguo , Lyu Liang , Chen Cheng , Yin Senlin , Jiang Shu , zhou peizhi 
Published: October 2021

Abstract
ObjectivesWe aimed to determine whether vertebral artery (VA) affects the success rate of microvascular decompression(MVD) in hemifacial spasm(HFS) patients. We compared the clinical presentations and surgical outcomes between VA-involved and non-VA HFS patients.MethodsThe study comprised 313 HFS patients who underwent MVD between January 2015 and December 2018. There were 59 patients in the VA-involved group and 254 patients in the non-VA group. The clinical results mainly included the rate of HFS remission and the complications. Postoperative neurosurgery-related problems and neurological issues were among the complications.ResultsAmong the 313 enrolled patients, 288 were spasem-free: 52 (88%) in VA-involved group and 236 (91.4%) in non-VA group. Delayed and partial remissions were frequent in VA-involved group. Furthermore, VA was significantly associated with partial remission (P=0.014). The total rate of complications was comparable between the two groups. However, motor or sensory impairments mainly occurred in the VA-involved group.ConclusionsThe rates of long-term remission and overall complication after MVD were not significantly different between the groups. VA-involved HFS may be at high risk for severe neurological complications and the partial spasem-free.
Unrelated Umbilical Cord Blood Transplantation For Children With Hereditary Leukodystrophy: Single Center Experience
Authors: Wang Ping , Du Xiaonan , Shen Quanli , Jiang Wenjin , Shen Chen , Wang Hongsheng , Zhou Shuizhen , Wang Yi , Qian Xiaowen , Zhai Xiaowen 
Published: October 2021

Abstract
Background

Hereditary leukodystrophies are diverse metabolic diseases caused by gene mutations that result in the abnormal development or degeneration of myelin. Transplantation of matched sibling donor umbilical cord blood (UCB) can slow progression and prolong survival in some cases. For patients without siblings, however, UCB transplantation from unrelated donors may be the only option.
Methods

This retrospective study assessed unrelated UCB transplantation (UCBT) efficacy following busulfan- and cyclophosphamide-based myeloablative chemotherapy.
Results

The study cohort included 12 pediatric patients (ten males), nine with adrenoleukodystrophy (ALD) and three with globoid cell leukodystrophy (GLD), treated between April 2015 and March 2020. All received HLA-matched or partially mismatched UCBT. Median age at UCBT was 7.2 years (range, [0.8–12.9 years]). There were no cases of graft rejection. Median neutrophil engraftment time was 20 days [12–33 days] and median platelet engraftment time was 29 days [14–65 days]. Median follow-up was 28 months [1–73 months], and overall survival rate was 83.3% (10/12). Seven patients had higher Loes scores post-transplantation, and two patients died of infection. Four patients with rapid neurological deterioration pre-UCBT exhibited worse neurological symptoms post-UCBT. In contrast, four patients with stable neurological symptoms pre-UCBT demonstrated symptom stability post-UCBT, and two with no neurological symptoms pre-UCBT were also symptom-free post-UCBT. Further, lipid profiles of surviving ALD patients were improved post-treatment.
Conclusions

Hereditary leukodystrophy patients with mild neurological symptoms can benefit from UCBT, while UCBT cannot reverse advanced disease.
Conditional Knockout of PDK1 in Osteoclasts Suppressed Osteoclastogenesis and Ameliorated Prostate Cancer-Induced Osteolysis
Authors: Zhang Yanan , Nong Haibin , Bai Yiguang , Zhou Quan , Zhang Qiong , Liu Mingfu , Liu Pan , Zeng Gaofeng , Zong Shaohui 
Published: October 2021

Abstract
Background: The development and maintenance of normal bone tissue is supported by balanced communication between osteoblasts and osteoclasts. The invasion of cancer cells disrupts this balance, leading to osteolysis. As the only bone resorption cells in vivo, osteoclasts play important roles in cancer-induced osteolysis. However, the function of 3-phosphoinositide–dependent protein kinase-1 (PDK1) in osteoclast resorption remains unclear. Methods: In our study, we used a receptor activator of nuclear factor-kappa B (RANK) promoter‐driven Cre‐LoxP system to conditionally delete the PDK1 gene in osteoclasts in mice. We investigated the impact of Osteoclast‐specific knockout of PDK1 on prostate cancer-induced osteolysis. Bone marrow-derived macrophage cells (BMMs) were extracted and induced to differentiate osteoclasts in vitro to examine the function of PDK1 in osteoclasts.Results: In this study, we found that PDK1 conditional knockout (cKO) mice exhibited smaller body sizes when contrasted with the wild-type (WT) mice. Moreover, deletion of PDK1 in osteoclasts ameliorated osteolysis and reduced bone resorption markers in the murine model of prostate cancer-stimulated osteolysis. In vivo, we discovered that osteoclast‐specific knockout of PDK1 suppressed RANKL-stimulated bone resorption function, osteoclastogenesis, and osteoclast-specific gene expression (Ctsk, TRAP, MMP-9, NFATc1). Western blot analyses of RANKL-induced signaling pathways showed that conditional knockout of PDK1 in osteoclasts inhibited the early nuclear factor κB (NF-κB) activation, which consequently suppressed the downstream induction of NFATc1. Conclusion: These findings demonstrated that PDK1 performs an instrumental function in osteoclastogenesis and prostate cancer-induced osteolysis by modulating the PDK1/AKT/NF-κB signaling pathway.
Multi-station calibration strategy for evaluation and sensitivity analysis of the snowmelt runoff model using MODIS satellite images
Authors: Nourani Vahid , Afkhaminia Amin , Andaryani Soghra , Zhang Yongqiang 
Published: October 2021

Abstract
In this study, the snowmelt runoff model (SRM) was employed to estimate the effect of snow on the surface flow of Aji-Chay basin, northwest Iran. Two calibration techniques were adopted to enhance the calibration. The multi-station calibration (MSC) and single-station calibration (SSC) strategies applied to investigate their effects on the modeling accuracy. The runoff coefficients (cs and cr) were selected as calibration parameters because of their uncertainty in such an extended basin. To determine the most substantial input of the model which is the snow-covered area (SCA) from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor imagery, MOD10A2 images were collected with spatial and temporal resolutions of 500 meters and 8 days, respectively. The results show an average of 15% improvement in the model performance in the MSC strategy from the data period of 2008–2012. Also, an appropriate agreement with physical characteristics of the study area could be seen for the calibration parameters. The contribution of snowmelt in the river flow reaches its peak in April and May, then with increasing temperature, the contribution decreased gradually. Furthermore, analysis of parameters indicates that the SRM is sensitive to recession coefficient and runoff coefficients.
Une statue à Divicot
Authors: Roorda Henri 
Published: October 2021

Dans cette chronique de 1920, Roorda donne libre cours à ses convictions pacifistes à partir de la figure de Divico, personnage de l’ancienne Helvétie nimbé de légende, dont il suggère ironiquement qu’il soit statufié pour être déclaré héros national et alimenter ainsi les sentiments guerriers chez les Suisses.
Keywords : Henri Roorda, guerre, pacifisme, Suisse, Divico, Charles Gleyre, Giovanni Giolitti.
Max Stirner et Henri Roorda sur l’éducation : éloge de l’indocilité
Authors: L’Aminot Tanguy 
Published: October 2021

Le premier texte que publie Roorda sous son nom, en 1898, s’intitule « L’École ou l’apprentissage de la docilité ». Le thème s’inscrit dans la critique anarchiste du pouvoir et trouve chez Max Stirner un précurseur. Celui-ci, dans Le Faux Principe de notre éducation notamment, a critiqué fortement le système scolaire et s’oppose à la soumission de l’enfant qui anticipe celle de l’adulte, en appelant à l’insoumission des enfants contre le maître. L’article examine donc successivement ce que chacun des deux auteurs dit à ce propos, puis propose une illustration du thème à travers plusieurs livres et films des années 1950 à 1970 : La Guerre des boutons et La Foire aux cancres, St Trinian’s et if….
Keywords : Henri Roorda, Max Stirner, éducation, indocilité, Louis Pergaud, cinéma, St Trinian’s, if….
Tradición poética y variante humana en "El rayo que no cesa", de Miguel Hernández: metáforas de lo invisible
Authors: Ferris José Luis 
Published: October 2021

El rayo que no cesa, segunda obra publicada de Miguel Hernández, ha sido sometida durante décadas a numerosos estudios críticos que han tratado de desentrañar el carácter clásico y vanguardista de esta obra, sus precedentes en la poesía áurea y moderna, tanto en forma como en contenido. Sin embargo, el ingrediente biográfico, determinante en la poesía de Hernández –en él es imposible deslindar vida y obra–, se ha mantenido al margen de estas consideraciones. El presente trabajo trata de aportar a los tradicionales estudios la variante humana apoyándose en nuevos y contrastados datos biográficos que desde 2002 forman parte de una investigación personal. El objetivo es sumar, a los conocimientos filológicos, una visión iluminadora de las circunstancias que motivaron El rayo que no cesa.
Keywords: poesía, Hernández, soneto, amor, petrarquismo, símbolo.
Agrupamientos poblacionales en Uruguay inferidos en base a la distribución de apellidos
Authors: Rodríguez-Larralde Alvaro , Dipierri José Edgardo , Alfaro Emma Laura , Sans Mónica 
Published: October 2021

Recientemente publicamos el análisis de los apellidos del Padrón Electoral de 2010 de la República Oriental del Uruguay a través de distancias isonímicas, basado en sus 19 departamentos y las 171 comparaciones posibles entre estos, lo que mostró la forma-ción de dos grandes agrupamientos en el sur, y otros pocos vinculados en el norte y este. El objetivo del presente trabajo es detallar el análisis de las distancias isonímicas utilizando la distribución de apellidos, no a nivel departamental, sino de los 221 municipios en los cuales se divide el país. Se estimaron las distancias isonímicas entre ellos, tomados dos a dos (24.310 comparaciones), se construyeron las matrices de distancia correspondientes y se correlacionaron con las de distancias geográficas. La correlación fue mayor para la distancia euclídea (r=0,253±0,033), intermedia para la de Lasker (r=0,18±0,029) y menor para la de Nei (r=0,17±0,03), todas estadísticamente significativas (p<0,001). Se construyeron nuevos dendrogramas determinándose agrupamientos no identificados previamente. Se destaca cierta homogeneidad en el sur con las me-nores distancias, con 7 agrupamientos cercanos a Montevideo. Al norte, con mayores distancias que denotan mayor heterogeneidad de apellidos, se identifican 8 agrupamientos. Se corrobora que el río Negro actuó como barrera geográfica, pero también se observan límites marcados por otros accidentes orográficos, hidrográficos, históricos y factores sociodemográficos no identificados en el análisis anterior. Por último, se resalta la utilidad del uso de apellidos para analizar la estructura poblacional, permitiendo el análisis global de la población de un país, en este caso, Uruguay.
Role of the Patient‘s Viewpoint in Health Technologies Assessment in Bulgaria
Authors: Georgieva C. , Yanakieva A. 
Published: October 2021

Abstract

Objectives: The aim of this study was to determine the role of the patient’s point of view when assessing health technologies as well as the patients’ willingness to participate in this process in our country.

Methods: A pilot study included 165 patients across Bulgaria recruited from December 2019 to March 2020. Appropriate descriptive and statistical methods were used.

Results: The patient’s point of view was an essential element in HTA. Patients in Bulgaria have the desire and willingness to share their personal experience with institutions, but insufficient knowledge of the process hinders their full participation in HTA. Our results showed that the need for higher awareness in the field and familiarity with HTA methodology are critical factors for the inclusion of patients in the HTA procedures in Bulgaria.

Conclusion: This study was one of the few ones focused on the impact of patient participation in HTA in Bulgaria. The patient’s role in the development of medical technology must be recognized and considered a critical factor in the future of HTA.