bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ulinastatin Inhibits Nasopharyngeal Carcinoma Metastasis by Suppressing uPA/uPAR&nbsp;Signaling
Authors: Li Chang-Zhi , Qiang Yuan-Yuan , Liu Zhi-Jie , Zheng Li-Sheng , Peng Li-Xia , Mei Yan , Meng Dong-Fang , Wei Wen-Wen , Chen Dong-Wen , Xu Liang , Lang Yan-Hong , Xie Ping , Peng Xing-Si , Wang Ming-Dian , Guo Ling-Ling , Xie De-Huan , Shu Di-Tian , Ding Liu-Yan , Lin Si-Ting , Luo Fei-Fei , Wang Jing , Li Sha-Sha , Huang Bi-Jun , Chen Jin-Dong , Qian Chao-Nan 
Published: April 2021

Abstract
Background: Distant metastasis is the main reason for treatment failure in patients with nasopharyngeal carcinoma (NPC). In this study, we evaluated the effect of ulinastatin (UTI) on NPC metastasis and investigated its underlying mechanism.Methods: Highly-metastatic NPC cell lines S18 and 5-8F were treated with UTI and the effect on cellular growth, migration, and invasion of NPC cells was determined using MTS and transwell assays. The luciferase-expressing S18 cells (S18-1C3) were injected into the left hind footpad of nude mice to establish a spontaneous footpad to popliteal lymph node (LN) metastasis model. The luciferase mRNA was measured by qPCR to calculate the metastatic inhibition rate. The UTI-related uPA, uPAR and the main molecular members of the JAT/STAT3 pathway were detected by qPCR and immunoblotting.Results: UTI inhibited the migration and invasion of S18 and 5-8F cells with no effect on cellular growth in vitro, and suppressed metastasis of S18 cells in vivo. uPAR expression was reduced from 24 to 48 hours after UTI treatment. The anti-metastasis effect of UTI partially relies on the suppression of uPA and uPAR.Conclusion: UTI partially inhibits NPC metastasis via downregulating the expression of uPA and uPAR.
Zoning forest fire risk at a county-level based on geographically weighted logistic regression: a case study of Wuyishan, China
Authors: You Weibin , Liu Wan , Yu Yunqin , He Dongjin 
Published: April 2021

Abstract
Identification of the fire risk area at the county level is the key spatial unit for local forest resource protection and fire prevention. However, current methods and standards focus on the rating of risk level at a national scale which are not necessarily applicable at a local level. Wuyishan is a county-level city in southeastern China, which is rich in forest resources and is important for biodiversity conservation. We used a binary logistic regression (BLR) model and a geographically weighted logistic regression (GWLR) model to examine the indicators of forest fire occurrence and map the forest risk zones in the study area based on historical fire survey data from 1999 to 2013. The results showed that the BLR model simulation found that four indicators (daily average relative humidity, daily sunshine hours, elevation, and distance to the closest railway) had a significant impact on the risk of forest fires in Wuyishan City. Daily sunshine hours had a positive correlation with forest fire risk, and the other three factors were negatively correlated. The GWLR model incorporated the spatial heterogeneity of indicators into the simulation and further demonstrated that only daily average relative humidity was correlated over the entire study area. In contrast, daily sunshine hours, elevation, and distance to the closest railway were effective indicators of fire risk at a local level. The prediction accuracy of the GWLR model (85.3%) was slightly higher than that of the BLR model (84.4%). Around 19.9% of the study area was in a high fire risk zone, 34.0% was in a medium-risk zone, and 46.1% was in a low-risk zone. The high-risk zones were mainly concentrated in the central and southern areas. Our results indicate that, during the fire prevention period, the forest fire management department needs to increase the frequency of daily inspections of the forest edge areas in the high- and medium-risk areas based on the fire risk zoning map. Our approach may improve the identification of forest fire risk and fire prevention and suppression management at a county-level in mountainous and hilly areas.
The Effect of Health Education via Mobile Apps in Promoting Asthma Health Related Quality of Life Among Asthmatic School Children in Urban Malaysia.
Authors: Raimi Abdulaziz Mansoor Al , Chong Chan Mei , Tang Li Yoong , Chua Yan Piaw , Ajeel Latifa Yahya Al 
Published: April 2021

Abstract
Objectives: The objective of study to assess the effect of health education via mobile applications in promoting health-related quality of life among schoolchildren with asthma in urban Malaysia. Methods: A Quasi-experimental with two-group pre- and post-intervention design was used in this study involving a total of 214 students. The students were randomly assigned into two groups (intervention group and control group) in a pre and post intervention approach. The control group received face to face education and the experimental group had health education via mobile apps.Results: The findings showed that the total score of health related quality of life has improvement in the mean total score of health-related quality of life from pre-intervention (5.31±1.27) to post-intervention (5.66±1.28) for the control group, compared with the experimental group with a mean total score of HRQoL at pre-intervention (5.01±1.36) and post-intervention (5.85±1.29). A comparison between the experimental and control groups using an independent t-test showed statistically significant differences in the mean HRQoL scores of asthma between the experimental and control groups. The effect of health education via mobile applications showed statistically significant improvement pre and post intervention in HRQoL score [F (1,288) = 57.46, p = <0.01].Conclusion: The use of mobile technology in health education improved HRQoL as compared of traditional method of face-to-face lecture or handbooks among school children with asthma. Thus, educational module using mobile apps improves HRQoL. Trial registration: This study was registered under the Medical Research Committee, University Malaya Medical Centre, Malaysia under Trial MRECID. NO: 2016112-4501, Also, this study is registered with the Australian New Zealand Clinical Trials Registry (ANZCTR) under Trial ID: ACTRN12614300582550
High expression of RRM2 promotes the pathogenesis of malignant ovarian endometriosis.
Authors: Yang Binkai , Hu Yuanjing , Wang Tian , Li Na , Zhang Wenwen 
Published: April 2021

Abstract
Objective: Our objective was to investigate the upregulated expression of ribonucleotide reductase M2 (RRM2) in the ectopic endometrium (EC) of ovarian endometriosis (OE) patients that may indicate malignant transformation. RRM2 may be used as a marker of OE, which contribute to the research of the mechanism of the malignant transformation of OE.Methods: The gene expression profiles of ovarian cancer and OE were downloaded from Gene Expression Omnibus (GEO), and a common hub gene, RRM2, was identified. The expression of RRM2 was low in OE and high in ovarian cancer. A total of 44 patients with endometriosis-associated ovarian cancers (EAOC) and 44 with OE were enrolled in this study. Immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of RRM2, while the relationship between RRM2 and Ki-67 was analyzed by IHC co-localization. Results: There was no significant difference in the expression of RRM2 in the eutopic endometrium (EU), EC, and cancer tissues of EAOC patients. Compared with OE patients, the mRNA and protein expression levels of RRM2 were higher in the EC of EAOC patients (p＜0.01). Moreover, the high expression of RRM2 was consistent with the expression of Ki-67 in EC of EAOC patients.Conclusions: The upregulated expression of RRM2 in the EC of OE patients may indicate malignant transformation. RRM2 may be used as a marker of OE, which allows the investigation of the mechanism of the malignant transformation of OE.
The IRF family can influence tumor immunity and the prognosis of patients with colorectal cancer
Authors: Chen Yan-Jie , Luo Shu-Neng , Dong Ling , Liu Tao-Tao , Shen Xi-Zhong , Zhang Ning-Ping , Liang Li 
Published: April 2021

Abstract
Background: The roles of interferon-regulatory factors (IRFs) in colorectal cancer (CRC) have not been studied through bioinformatics analysis. Methods: We used gene- and microRNA-expression data for patients with somatic mutations and colon adenocarcinoma/rectum adenocarcinoma from The Cancer Genome Atlas Genomic Data Commons as a training dataset. Gene-expression data (accessions GSE17536 and GSE39582) were downloaded from the Gene Expression Omnibus database as the validation dataset. Expressional differences, clinical correlations, disease prognosis, functional enrichment, and immune correlations of IRF genes were analyzed. The results were validated via immunohistochemistry. Results: The mRNA-expression levels of IRF3 and IRF7 differed between tumor and normal tissues and were correlated with patient prognosis. The IRF score was an independent risk factor for overall survival. IRFs recruited inflammatory cells; however, the immune and stromal scores showed inflammatory-cell recruitment only in the tumor stroma; therefore, they did not help eliminate tumor cells. Functional-enrichment analysis and pan-cancer expression analysis revealed that IRFs were differentially expressed in tumor tissues and associated with patient prognosis. Conclusions: IRFs were differentially expressed in tumor tissues and were associated with prognosis in CRC patients. Although IRFs can promote the infiltration of immune cells, the immune and stromal score showed that the infiltrated immune cells mostly stayed in the tumor stroma and cannot directly eliminate the tumor. Our findings can help to improve CRC prognosis and treatment strategies.
The impact of visiting operational departments on improving public health students’ learning about Rabies preventions, coetaneous Leishmaniasis, and serological tests of Brucellosis: a learning trip
Authors: Charkazi Abdurrahman 
Published: April 2021

Abstract
Background: Conventional teaching through lectures cannot be of much use and efficiency for achieving the educational goals of this course. Designing community-based teachings could be influential in students’ learning quality. Hence, the present study was conducted aiming to evaluate the impact of visiting the operational departments of Gonbad-e Kavous county healthcare center on improving public health students’ learning of educational objectives of the national infections disease combat program. Methods: Over a cross-sectional study, 97 students of continues and discontinuous bachelor’s majoring in public health visited the department of rabies prevention and control, coetaneous Leishmaniasis(CL) control, and the central laboratory of Gonbad-e Kavous county healthcare center over the first semester of the 2017-19 academic year. These students were introduced to the methods of treating Leishmaniasis patients and those bitten by animals as well as brucellosis diagnosis tests. The students were provided with the study's questionnaire after the visit so that they could fill it in. collected data entered SPSS v.15 statistical software and were analyzed using descriptive statistics and an independent t-test. Results: 61 (62.9%) of the students were female and 50 (51.5%) people were discontinuous bachelor’s students. The average satisfaction of students with the CL, rabies control and prevention, and Brucellosis test departments was 93.38%, 89.25%, and 82.69%, respectively. Besides, the total satisfaction of the mentioned visits was 92.61%. Discontinuous bachelor's students' satisfaction with rabies control and prevention and Brucellosis departments was significantly higher compared to continuous bachelor's students (P<0.05). Conclusion: results indicated that students’ satisfaction with visiting the operational departments of Gonbad-e Kavous county healthcare center was high. Hence, further such visits are recommended for other public health students.
You Gotta Walk the Walk to Talk the Talk: Happy Older Latino Adults (HOLA) as a pilot health promotion and prevention program for older HIV-positive Latino men
Authors: Jimenez Daniel , Weinstein Elliott R. , Batsis John 
Published: April 2021

Abstract
Background
Older Latinos with HIV have been disproportionately affected by the epidemic and experience compounded health disparities that have deepened over time. These health disparities are largely related to lifestyle and are either preventable or amenable to early detection or intervention. Despite existing resources to deliver an intervention to reduce this compounded health disparity, there is little information on the effects of health promotion interventions on indices of cardiometabolic risk in midlife and older Latinos with HIV. The Happy Older Latinos are Active (HOLA) intervention is an innovative health promotion program that is uniquely tailored to meet the diverse needs and circumstances of older Latinos with HIV. The goal of this pilot study is to evaluate the feasibility and acceptability of HOLA among Latinos aged 50 and older with HIV.
Methods/Design:
HOLA, which is informed by Behavioral Activation and Social Learning theory is a community health worker (CHW)-led, multicomponent, health promotion intervention consisting of: (1) a social and physical activation session; (2) a moderately intense group walk led by a CHW for 45 minutes, 3x/week for 16 weeks; (3) pleasant events scheduling. The proposed pilot study will recruit and enroll 18 community dwelling Latinos with HIV aged 50+. Participants will be assessed at three time points (baseline, post intervention, and 3 months post intervention) on measures of cardiometabolic risk factors (waist circumference, dyslipidemia, hypertension, and glucose), psychosocial functioning, and health-related quality of life.
Conclusions
If HOLA can be delivered successfully by CHWs, then the scalability, accessibility, and potential for dissemination is increased. Additionally, the results of this pilot study have far reaching implications in regard to the health and welfare of other at-risk, under resourced populations. This pilot study will inform feasibility and identify modifications needed in the design of a larger hypothesis testing study.
Combination of dabrafenib with irinotecan might trigger off a higher frequency of adverse events by pharmacokinetic interaction
Authors: Wang Zhe , Wang Xiaoyu , Wang Zhen , Fan Xiaoyu , Yan Mingrui , Jiang Lili , Xia Yangliu , Cao Jun , Liu Yong 
Published: April 2021

Abstract
Dabrafenib and irinotecan are two drugs that can be utilized to treat melanoma. A previous in vivo study has shown that dabrafenib enhances the antitumor activity of irinotecan in a xenograft model with unclear mechanism. This study aims to investigate the inhibition of dabrafenib on SN-38 (the active metabolite of irinotecan) glucuronidation using human liver microsomes (HLMs) and recombinant human UGT1A1, trying to elucidate the possible mechanism underlying the synergistic effect. Our data indicated that dabrafenib noncompetitively inhibited SN-38 glucuronidation in pooled HLMs and recombinant UGT1A1 with a K i,u value was 12.43 ± 0.28 and 2.64 ± 0.27 μM, respectively. Based on the in vitro K i,u value and estimation of kinetic parameters, dabrafenib administered at 150 mg twice daily may result in about 17%-81.9% increase in the area under the curve (AUC) of SN-38 in vivo . Moreover, the ratios of [ I ] gut / K i,u are 0.70 and 3.32 in HLMs and recombinant UGT1A1, respectively, indicating a high risk of drug-drug interactions (DDIs) when dabrafenib was used in combination with irinotecan. Our study provides a basis for further development and optimization of this combination in clinical research.
Techno-Economic and Sensitivity Analysis of Biodiesel Production Using Co-Solvent
Authors: El-Galad M. , El-Khatib kamel , El-Sheltawy Shakinaz 
Published: April 2021

Abstract
Three different continuous homogeneous alkali-catalyzed, heterogeneous alkali-catalyzed and supercritical methanolysis processes with adding tetrahydrofuran as a co-solvent to produce biodiesel from virgin vegetable oil were designed and simulated. An economic assessment was also performed based on the results of the process simulations. Technical assessment of the proposed processes showed that the homogeneous and heterogeneous alkali-catalyzed processes were the simplest with the least amount of process equipment, while for the supercritical methanolysis process; it was more complex with larger sizes of transesterification and separation units. The homogeneous alkali-catalyzed process using tetrahydrofuran was found to have the lowest total capital cost ($2.32 million), the highest aftertax rate of return (513%) and the payback period (0.19 year). However, total manufacturing cost and aftertax net profit, the heterogeneous alkali-catalyzed process using tetrahydrofuran had the lowest operating cost ($82.20 million) and the highest aftertax net profit ($18.20 million). The homogeneous alkali-catalyzed process I is not only technically feasible but also economically viable. Results from the sensitivity analyses indicated that methanol recovery percent, biodiesel purification tower vacuum pressure, price of feedstock oil, price of methanol, price of biodiesel and price of glycerin by-product were the factors that most significantly affected the economic feasibility of biodiesel production.
Investigating degradation metabolites and underlying pathway of azo dye “Reactive Black 5” in bioaugmented floating treatment wetlands
Authors: Tara Nain , Iqbal Mazhar , Habib Fazale , Khan Qaiser Mahmood , Iqbal Samina , Afzal Muhammad , Brix Hans 
Published: April 2021

Abstract
The direct discharge of azo dyes and/or their metabolites into the environment may exert toxic, mutagenic, and carcinogenic effects on exposed fauna and flora. In this study, we analyzed the metabolites produced during the degradation of an azo dye namely Reactive Black 5 (RB5) in the bacterial augmented-floating treatment wetlands (FTWs), followed by the investigation of their underlying toxicity. To this end, a FTWs system was developed by using a common wetland plant Phragmites australis in the presence of three dye-degrading bacteria (Acinetobacter junii strain NT-15, Pseudomonas indoloxydans strain NT-38, and Rhodococcus sp. strain NT-39). We found that the FTW system effectively degraded RB5 into at least 20 different metabolites with the successful removal of color (95.5%) from the water. The fish toxicity assay revealed the non-toxic characteristics of the metabolites produced after dye degradation. Our study suggests that bacterially aided FTWs could be a suitable option for the successful degradation of azo dyes, and the results presented in this study may help improve the overall textile effluent clean-up processes.
Giant Coronary Artery Aneurysms Involving More Than One Coronary Artery
Authors: Khouzam Matthew S , Khouzam Nayer 
Published: April 2021

Abstract
Background: Coronary artery aneurysms are rare findings in patients undergoing coronary angiography. The presence of multiple coronary artery aneurysms located in more than one coronary artery is even more uncommon. The pathophysiology of such aneurysms is unknown, but the majority are often due to atherosclerosis, congenital heart disease, or vasculitis. Case Presentation: We present a rare case of a 78-year-old female patient who presented with unstable angina and non-ST segment elevation myocardial infarction. On coronary angiography she was found to have three separate 1 cm saccular aneurysms involving the proximal left anterior descending coronary artery. The right coronary artery could not be visualized. Computed chest tomography revealed a 6.6 x 6.3 cm saccular aneurysm of the right coronary artery, and a 4.4 cm fusiform aneurysm of the ascending aorta. The patient gave no history of percutaneous coronary intervention or cardiac surgical procedures. She had a previous history of endovascular stenting of an abdominal aortic aneurysm. The sizable right coronary artery aneurysm showed extrinsic compression of both the right atrium and ventricle with right ventricular hypokinesis. Serological studies for vasculitis were all negative. Pathology of the aneurysm wall revealed calcific atherosclerosis without evidence of vasculitis. The patient underwent subtotal resection of the right coronary aneurysm with ligation of the proximal and distal ends of the right coronary artery and double bypass surgery to the left anterior descending and right posterior descending coronary arteries. Conclusion: The presence of multiple, large coronary artery aneurysms is very rare. Treatment can be challenging and should be individualized. Surgical treatment is recommended for giant coronary artery aneurysms to prevent potential complications. Keywords: coronary artery aneurysm, aortic aneurysm, atherosclerosis, non-ST segment 32 elevation myocardial infarction, case report
Fermi blockade of the strong electron-phonon interaction in optimally doped high temperature superconductors
Authors: Mishchenko Andrey , Nagaosa Naoto , Prokof'ev Nikolay , Tupitsyn Igor 
Published: April 2021

Abstract
We study how manifestations of strong electron-phonon interaction (EPI) depend on the carrier concentration by solving the two-dimensional Holstein model for the spin-polarized fermions using an approximation free bold-line diagrammatic Monte Carlo (BDMC) method. We show that the strong EPI, obviously present at very small Fermion concentration, is masked by the Fermi blockade effects and Migdal's theorem to the extent that it manifests itself as moderate one at large carriers densities. Suppression of strong EPI fingerprints is in agreement with experimental observations in doped high temperature superconductors.