bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peritraumatic Plasma Omega-3 Fatty Acid Concentration Predicts Chronic Pain Severity Following Thermal Burn Injury
Authors: Mauck Matthew C , Barton Chloe E , Tungate Andrew S , Shupp Jeffrey W , Karlnoski Rachel , Smith David J , Williams Felicia N , Jones Samuel W , Sefton Christopher , McGrath Kyle , Cairns Bruce A , McLean Samuel A 
Published: April 2021

Abstract
Chronic pain is a significant comorbidity of burn injury affecting up to 60% of survivors. Currently, no treatments are available to prevent chronic pain after burn injury. Accumulating evidence suggests that omega-3 fatty acids (O3FAs) improve symptoms across a range of painful conditions. In this study, we evaluated whether low peritraumatic levels of O3FA predict greater pain severity during the year after burn injury. Burn survivors undergoing skin autograft were recruited from three participating burn centers. Plasma O3FA (n = 77) levels were assessed in the early aftermath of burn injury using liquid chromatography/mass spectrometry, and pain severity was assessed via the 0 to 10 numeric rating scale for 1 year following burn injury. Repeated-measures linear regression analyses were used to evaluate the association between peritraumatic O3FA concentrations and pain severity during the year following burn injury. Peritraumatic O3FA concentration and chronic pain severity were inversely related; lower levels of peritraumatic O3FAs predicted worse pain outcomes (β = −0.002, P = .020). Future studies are needed to evaluate biological mechanisms mediating this association and to assess the ability of O3FAs to prevent chronic pain following burn injury.
Essentialism and Exclusion: Racism in Cancer Risk Prediction Models
Authors: Waters Erika A , Colditz Graham A , Davis Kia L 
Published: April 2021

AbstractCancer risk prediction models have the potential to revolutionize the science and practice of cancer prevention and control by identifying the likelihood that a patient will develop cancer at some point in the future, likely experience more benefit than harm from a given intervention, and survive their cancer for a certain number of years. The ability of risk prediction models to produce estimates that are valid and reliable for people from diverse socio-demographic backgrounds—and consequently their utility for broadening the reach of precision medicine to marginalized populations—depends on ensuring that the risk factors included in the model are represented as thoroughly and as accurately as possible. However, cancer risk prediction models created in the United States have a critical limitation, the origins of which stem from the country’s earliest days: they either erroneously treat the social construct of race as an immutable biological factor (ie, they “essentialize” race), or they exclude from the model those socio-contextual factors that are associated with both race and health outcomes. Models that essentialize race and/or exclude socio-contextual factors sometimes incorporate “race corrections” that adjust a patient’s risk estimate up or down based on their race. This commentary discusses the origins of race corrections, potential flaws with such corrections, and strategies for developing cohorts for developing risk prediction models that do not essentialize race or exclude key socio-contextual factors. Such models will help move the science of cancer prevention and control towards its goal of eliminating cancer disparities and achieving health equity.
Safe laparoscopic appendectomy in pregnant patient during active labor
Authors: Austin Charlotte S , Jaronczyk Michael 
Published: April 2021

AbstractAppendectomy is the standard of care in pregnant patients with acute appendicitis. The use of laparoscopy in pregnant patients with acute appendicitis is still debated, especially for patients in their third trimester. We present a case of a patient who safely underwent a laparoscopic appendectomy during early labor and subsequently delivered a healthy baby. In the correct situations and hands, laparoscopy can likely be safely used throughout pregnancy.
ArabidopsisSMC6AandSMC6Bhave redundant function in seed and gametophyte development
Authors: Zou Wenxuan , Li Gang , Jian Liufang , Qian Jie , Liu Yantong , Zhao Jie 
Published: April 2021

AbstractReproductive development is a crucial process during plant growth. The structural maintenance of chromosome (SMC) 5/6 complex has been studied in various species. However, there are few studies on the biological function of SMC6 in plant development, especially during reproduction. In this study, knocking out of both AtSMC6A and AtSMC6B led to severe defects in Arabidopsis seed development, and expression of AtSMC6A or AtSMC6B could completely restore seed abortion in the smc6a-/-smc6b-/-double mutant. Knocking down AtSMC6A in the smc6b-/- mutant led to defects in female and male development and decreased fertility. The double mutation also resulted in loss of cell viability, and caused embryo and endosperm cell death through vacuolar cell death and necrosis. Furthermore, the expression of genes involved in embryo patterning, endosperm cellularisation, DNA damage repair, cell cycle regulation, and DNA replication were significantly changed in the albino seeds of the double mutant. Moreover, we found that the SMC5/6 complex may participate in the SOG1 (SUPPRESSOR OF GAMMA RESPONSE1)-dependent DNA damage repair pathway. These findings suggest that both AtSMC6A and AtSMC6B are functionally redundant and play important roles in seed and gametophyte development through maintaining chromosome stability in Arabidopsis.
Orchestration of plant development and defense by indirect crosstalk of salicylic acid and brassinosteorid signaling via transcription factor GhTINY2
Authors: Xiao Shenghua , Hu Qin , Zhang Xiaojun , Si Huan , Liu Shiming , Chen Lin , Chen Kun , Berne Sabina , Yuan Daojun , Lindsey Keith , Zhang Xianlong , Zhu Longfu 
Published: April 2021

Abstract
Salicylic acid (SA) and brassinosteroids (BRs) are well known to regulate diverse processes of plant development and stress responses, but the mechanisms by which these phytohormones mediate the growth and defense trade-off are largely unclear. In addition, little is known about the roles of DEHYDRATION RESPONSIVE ELEMENT BINDING transcription factors, especially in biotic stress and plant growth. Here, we identified a cotton (Gossypium hirsutum) APETALA2/ETHYLENE RESPONSIVE FACTOR gene GhTINY2 that is strongly induced by Verticillium dahliae. Overexpression of GhTINY2 in cotton and Arabidopsis enhanced tolerance to V. dahliae, while knockdown of expression increased the susceptibility of cotton to the pathogen. GhTINY2 was found to promote SA accumulation and SA signaling transduction by directly activating expression of WRKY51. Moreover, GhTINY2-overexpressing cotton and Arabidopsis showed retardation of growth, increased sensitivity to inhibitors of BR biosynthesis, down-regulation of several BR-induced genes, and up-regulation of BR-repressed genes, while GhTINY2-RNAi cotton showed the opposite effects. We further determined that GhTINY2 negatively regulates BR signaling by interacting with BRASSINAZOLE-RESISTANT 1 (BZR1) and restraining its transcriptional activation of the expression of INDOLE-3-ACETIC ACID INDUCIBLE 19 (IAA19). These findings indicate that GhTINY2 fine-tunes the trade-off between immunity and growth via indirect crosstalk between WRKY51-mediated SA biosynthesis and BZR1-IAA19-regulated BR signaling.
PERCEPTRON: an open-source GPU-accelerated proteoform identification pipeline for top-down proteomics
Authors: Khalid Muhammad Farhan , Iman Kanzal , Ghafoor Amna , Saboor Mujtaba , Ali Ahsan , Muaz Urwa , Basharat Abdul Rehman , Tahir Taha , Abubakar Muhammad , Akhter Momina Amer , Nabi Waqar , Vanderbauwhede Wim , Ahmad Fayyaz , Wajid Bilal , Chaudhary Safee Ullah 
Published: April 2021

AbstractPERCEPTRON is a next-generation freely available web-based proteoform identification and characterization platform for top-down proteomics (TDP). PERCEPTRON search pipeline brings together algorithms for (i) intact protein mass tuning, (ii) de novo sequence tags-based filtering, (iii) characterization of terminal as well as post-translational modifications, (iv) identification of truncated proteoforms, (v) in silico spectral comparison, and (vi) weight-based candidate protein scoring. High-throughput performance is achieved through the execution of optimized code via multiple threads in parallel, on graphics processing units (GPUs) using NVidia Compute Unified Device Architecture (CUDA) framework. An intuitive graphical web interface allows for setting up of search parameters as well as for visualization of results. The accuracy and performance of the tool have been validated on several TDP datasets and against available TDP software. Specifically, results obtained from searching two published TDP datasets demonstrate that PERCEPTRON outperforms all other tools by up to 135% in terms of reported proteins and 10-fold in terms of runtime. In conclusion, the proposed tool significantly enhances the state-of-the-art in TDP search software and is publicly available at https://perceptron.lums.edu.pk. Users can also create in-house deployments of the tool by building code available on the GitHub repository (http://github.com/BIRL/Perceptron).
Sleep deprivation reduces vagal tone during an inspiratory endurance task in humans
Authors: Westphal Willy-Paul , Rault Christophe , Robert René , Ragot Stéphanie , Neau Jean-Philippe , Fernagut Pierre-Olivier , Drouot Xavier 
Published: April 2021

AbstractStudy ObjectivesSleep deprivation alters inspiratory endurance by reducing inspiratory motor output. Vagal tone is involved in exercise endurance. This study aimed to investigate the effect of sleep deprivation on vagal tone adaptation in healthy subjects performing an inspiratory effort.MethodsVagal tone was assessed using Heart Rate Variability normalized units of frequency domain component HF (high frequency) before, at the start, and the end of an inspiratory loading trial performed until exhaustion by 16 volunteers after one night of sleep deprivation and one night of normal sleep, where sleep deprivation reduced the inspiratory endurance by half compared to the normal sleep condition (30 min vs 60 min).ResultsAt rest, heart rate was similar in sleep deprivation and normal sleep conditions. In normal sleep condition, heart rate increased during inspiratory loading task; this increase was greater in sleep deprivation condition. In normal sleep condition, vagal tone increased at the beginning of the trial. This vagal tone increase was absent in sleep deprivation condition.ConclusionsSleep deprivation abolished vagal tone response to inspiratory load, possibly contributing to a higher heart rate during the trial and to a reduced inspiratory endurance.Clinical Trial RegistrationNCT02725190.
Developmental and Intergenerational Landscape of Human Circulatory Lipidome and its Association with Obesity Risk
Authors: Mir Sartaj Ahmad , Chen Li , Burugupalli Satvika , Burla Bo , Ji Shanshan , Smith Adam Alexander T. , Narasimhan Kothandaraman , Ramasamy Adaikalavan , Tan Karen Mei-Ling , Huynh Kevin , Giles Corey , Mei Ding , Wong Gerard , Yap Fabian , Tan Kok Hian , Collier Fiona , Saffery Richard , Vuillermin Peter , Bendt Anne K. , Burgner David , Ponsonby Anne-Louise , Lee Yung Seng , Chong Yap Seng , Gluckman Peter D , Eriksson Johan G. , Meikle Peter J. , Wenk Markus R. , Karnani Neerja 
Published: April 2021

AbstractLipids play a vital role in human health and development, but changes to their circulatory levels during gestation and in early life are poorly understood. Here we present the first developmental and intergenerational landscape of the human circulatory lipidome, derived by profiling of 480 lipid species representing 25 lipid classes, in mothers and their offspring (n=2491). Levels of 66% of the profiled lipids increased in maternal circulation during gestation, while cord blood had higher concentrations of acylcarnitines and lysophospholipids. The offspring lipidome at age six years revealed striking similarities with postnatal maternal lipidome (adult) in its lipid composition and concentrations. Comparison of lipids associated with child and maternal adiposity identified a 92% overlap, implying intergenerational similarities in the lipid signatures of obesity risk. We also catalogued lipid signatures linked with maternal adiposity during gestation and offspring birthweight, and validated (>70% overlap) the findings in an independent birth-cohort (n=1935).
Convergent, functionally independent signaling by mu and delta opioid receptors in hippocampal parvalbumin interneurons
Authors: He Xinyi Jenny , Patel Janki , Weiss Connor E. , Ma Xiang , Bloodgood Brenda L. , Banghart Matthew R. 
Published: April 2021

AbstractFunctional interactions between G protein-coupled receptors are poised to enhance neuronal sensitivity to neuromodulators and therapeutic drugs. Mu and Delta opioid receptors (MORs and DORs) can interact when overexpressed in the same cells, but whether co-expression of endogenous MORs and DORs in neurons leads to functional interactions is unclear. Here, we show that both MORs and DORs inhibit parvalbumin-expressing basket cells (PV-BCs) in hippocampal CA1 through partially occlusive signaling pathways that terminate on somato-dendritic potassium channels and presynaptic calcium channels. Using photoactivatable opioid neuropeptides, we find that DORs dominate the response to enkephalin in terms of both ligand-sensitivity and kinetics, which may be due to relatively low expression levels of MOR. Opioid-activated potassium channels do not show heterologous desensitization, indicating that MORs and DORs signal independently. In a direct test for heteromeric functional interactions, the DOR antagonist TIPP-Psi does not alter the kinetics or potency of either the potassium channel or synaptic responses to photorelease of the MOR agonist DAMGO. Thus, despite largely redundant and convergent signaling, MORs and DORs do not functionally interact in PV-BCs. These findings imply that crosstalk between MORs and DORs, either in the form of physical interactions or synergistic intracellular signaling, is not a preordained outcome of co-expression in neurons.
A Panel of DiversePseudomonas aeruginosaClinical Isolates for Research and Development
Authors: Lebreton Francois , Snesrud Erik , Hall Lindsey , Mills Emma , Galac Madeline , Stam Jason , Ong Ana , Maybank Rosslyn , Kwak Yoon I. , Johnson Sheila , Julius Michael , Swierczewski Brett , Waterman Paige E. , Hinkle Mary , Jones Anthony , Lesho Emil , Bennett Jason W. , McGann Patrick 
Published: April 2021

AbstractPseudomonas aeruginosais a leading cause of community-acquired and hospital-acquired infections. Successful treatment is hampered by its remarkable ability to rapidly develop resistance to antimicrobial agents, mostly through mutation. In response, the World Health Organization listed carbapenem-resistantP. aeruginosaas a Priority 1 (Critical) pathogen for research and development of new treatments. A key resource in developing effective countermeasures is access to diverse and clinically relevant strains for testing. Herein we describe a panel of 100 diverseP. aeruginosastrains to support this endeavor.Whole genome sequencing was performed on 3,785P. aeruginosahoused in our repository. Isolates were cultured from clinical samples collected from healthcare facilities around the world between 2003 and 2017. Core-genome multi-locus sequence typing and high-resolution SNP-based phylogenetic analyses were used to select a panel of 100 strains that captured the genetic diversity of this collection. Comprehensive antibiotic susceptibility testing was also performed using 14 clinically relevant antibiotics.This 100-strain diversity panel contained representative strains from 91 different sequence-types, including genetically distinct isolates from major epidemic clones ST-111, ST-235, ST-244, and ST-253. Seventy-one distinct antibiotic susceptibility profiles were identified ranging from pan-sensitive to pan-resistant. Known resistance alleles as well as the most prevalent mutations underlying the antibiotic susceptibilities were characterized for all isolates.This panel provides a diverse and comprehensive set ofP. aeruginosastrains for use in developing solutions to antibiotic resistance. The isolates, and all available meta-data including genome sequences, are available to industry, academic institutions, federal and other laboratories at no additional cost.ImportancePseudomonas aeruginosais one of the most important human pathogens and a leading target in the development of new drugs and therapeutics. This species displays a remarkable level of diversity and any potential therapeutic must contend with this characteristic to ensure it retains efficacy across different strains. To date, only limited panels ofP. aeruginosaare available for testing, and none have been designed to capture the genetic diversity of the species. The panel described herein has been designed to address this shortcoming by providing a set of 100 distinct strains that greatly captures the diversity of the species. This panel will be of significant value to research groups working on this important pathogen, both for research purposes and in the development of new diagnostics and countermeasures.
Multiple drug resistance in hookworms infecting greyhound dogs in the USA
Authors: Castro Pablo D. Jimenez , Venkatesan Abhinaya , Redman Elizabeth , Chen Rebecca , Malatesta Abigail , Huff Hannah , Zuluaga Salazar Daniel A. , Avramenko Russell , Gilleard John. S. , Kaplan Ray M. 
Published: April 2021

AbstractThe hookwormAncylostoma caninumis the most prevalent nematode parasite of dogs. Recently, we confirmed multiple-drug resistance (MDR) in severalA. caninumisolates to all anthelmintic drug classes approved for the treatment of hookworms in dogs in the United States (USA). Cases of MDR hookworms appear to be highly overrepresented in greyhounds, suggesting that the MDR worms evolved on racing greyhound farms/kennels. The aims of this study were to evaluate the range of drug-resistant phenotypes and genotypes of theA. caninuminfecting greyhounds. Fecal samples from recently retired greyhounds originating from geographically diverse areas of the USA were acquired from two greyhound adoption kennels, one active greyhound racing kennel, and three veterinary practices that work with adoption kennels. Fecal egg counts (FECs) were performed on fecal samples from 219 greyhounds, and despite almost all the dogs having been treated with one or more anthelmintics in the previous two to four weeks, the mean FEC was 822.4 eggs per gram (EPG). Resistance to benzimidazoles and macrocyclic lactones were measured using the egg hatch assay (EHA) and the larval development assay (LDA) respectively. We performed 23 EHA and 22 LDA on either individual or pooled feces, representing 81 animals. Mean and median IC50and IC95values for the EHA were 5.3 uM, 3.6 uM, and 24.5 uM, 23.4 uM respectively. For the LDA, mean and median IC50values were 749.8 nM, >1000 nM respectively. These values range from 62 to 68 times higher than those we measured in our susceptible laboratory isolates. Pre-treatment fecal samples could not be obtained, however, post-treatment samples representing 219 greyhounds were collected. For samples collected <10 days post-treatment with albendazole, moxidectin, or a combination of febantel-pyrantel-moxidectin, the mean FEC were 349, 333, and 835 EPG, respectively. Samples collected 10-21 days post-treatment with albendazole, moxidectin, or pyrantel, yielded mean FEC of 1874, 335, and 600 EPG, respectively. Samples collected >21 days post-treatment with albendazole or moxidectin yielded mean FEC of 1819 and 1117 EPG, respectively. We obtained DNA from hookworm eggs isolated from 70 fecal samples, comprised of 60 individual dogs and 10 pools from multiple dogs. Deep sequencing of the isotype 1 β-tubulin gene revealed the presence of the F167Y (TTC>TAC) resistance polymorphism in 99% of these samples, with 69% having ≥75% resistant allele frequency. No resistance-associated polymorphisms were seen at any of the other β-tubulin codons previously reported as associated with benzimidazole resistance in Strongylid nematodes. These clinical,in vitro, and genetic data provide strong evidence that racing and recently retired greyhound dogs in the USA are infected with MDRA. caninumat very high levels in terms of both prevalence and infection intensity.
Diet Potentially Drives the Differentiation of Eating Behaviours via Alterations to the Gut Microbiome in Infants
Authors: Yan Cathy , Zhao Helen , Nayar Navika , Rhee Kyung E. , Lumeng Julie C. 
Published: April 2021

ABSTRACTCertain infant eating behaviours are associated with adverse health outcomes such as obesity. While a diet consisting of infant formula has been linked to higher-risk eating behaviours and changes in the gut microbiome, little is known about what role the gut microbiome plays in mediating eating behaviours. Using 16S rRNA sequences extracted from 96 fecal samples collected from 58 infants, we identified a subset of bacterial taxa that were more abundant in formula-fed infants, primarily composed of the phylum Firmicutes. The presence of these taxa correlated with a lower drive to eat (i.e., lower food responsiveness). Furthermore, short-chain fatty acid production pathways were significantly more abundant in formula-fed infants, negatively correlated with food responsiveness, and positively associated with relative abundance of the Firmicutes subset. Our results suggest that higher abundances of Firmicutes in formula-fed infants may decrease their food responsiveness through short-chain fatty acid production in the first four months of life. Taken together, these findings suggest a potential role for the infant’s diet in impacting eating behaviour via changes to the gut microbiome, which may lead to the development of novel interventions for the prevention of childhood obesity.