bluuebunny/crossref_metadata_2025_split huggingface dataset:
Major Regularities of Transformation of the Small Mammal Ovaries in Unfavourable Environmental Conditions: Ecological and Morphological Aspects
Authors: Blinova E. V. , Shevlyuk N. N. 
Published: December 2021

The aim of the study was to determine the patterns of structural and functional organization of the ovaries of female small mammals inhabiting technogenically altered ecosystems.Material and methods. We studied the ovaries of small mammal species belonging to the insectivore and rodent families (common shrew, field and pygmy wood mice, common and bank voles, mole vole, steppe pied) that live in anthropogenically altered ecosystems (zones of influence of ferrous and nonferrous metallurgy, as well as gas processing factory). The resulting material was processed using observational histological, histochemical, immunohistochemical and morphometric tests.Results. The results obtained demonstrated that in technogenically altered ecosystems the intensified reproduction results in a complex of morphofunctional reactive and adaptive changes in the ovaries of females of the studied species. The size of the ovaries was reduced; the area of the cortical substance was reduced. In the cortex, there was revealed a decrease in the number of follicles varying over a wide range - from a moderate decrease to their almost complete absence. There was found a decrease in the area of the vessels of the microvasculature; this was one of the major reasons for the increased follicular atresia. In follicles of various types, there was an increase in the proportion of cells expressing the proapoptotic protein P53. A decrease in the number of follicles resulted in the connective tissue overgrowth. The presence of cysts lined with epithelium of various heights was revealed in the cortex and medulla. Conclusion. The results obtained evidence that in technogenically altered ecosystems a decreased ovarian reserve is observed in the ovaries of female small mammals; it is associated with a more rapid depletion of the follicle reserve in the cortex due to both - intensification of reproduction and more rapid death of follicles in unfavourable environmental conditions.
Applying Self-Directed Groupwork and Social Action Research Methodology with francophone parents of trans children and youth in Rural Quebec
Authors: Pullen Sansfaçon Annie , Pineault Dominique , Davis Jennifer , Dyer Jennifer , Julie James , Ens Manning Kimberley , Temple-Newhook Julia , Pickett Sarah 
Published: December 2021

Parental support is an important protective factor in preventing suicide among trans youth. However, parents often struggle to accept their child’s gender transition. Having access to a group and to meeting other parents facilitates the acceptance process, but support groups are rare and even more so outside urban areas. The research project discussed in this article was developed to understand specifically the experience of parents of francophone trans youth living in rural and remote communities in Quebec. A Self-Directed Group approach (Mullender, Ward and Fleming 2013), combined with Social Action Research (Author et al. 2015) was initiated to offer a platform for parents to explore the specificities of parenting a trans child in rural francophone Quebec. Parents were recruited through social and traditional media and a series of group meetings was planned to collect data and as well as to facilitate the parents' mobilization. Despite applying the Self-Directed Groupwork principles and process, many challenges were encountered, stimulating a number of reflections on the use of self-directed group work and social action in remote geographical areas. This paper explores some of those challenges and discusses a possible way forward for research.
PREDIKSI BERAT TUBUH SAPI PERAH FRIESIAN-HOLSTEIN MENGGUNAKAN MODEL VON BERTALANFFY
Authors: Larasati Niken , Sulistyoningrum Tri Puji , Estri Mutia Nur , Sihwaningrum Idha , Reorita Rina 
Published: December 2021

Pada makalah ini dibahas mengenai prediksi berat tubuh sapi perah Friesian-Holstein menggunakan model Von Bertalanffy. Laju metabolisme pada model terdiri dari anabolisme dan katabolisme. Prediksi berat tubuh sapi perah ini penting karena dapat digunakan untuk menentukan usia kawin pertama kali sapi perah FH. Usia kawin pertama yang tidak tepat dapat menyebabkan produksi susu yang rendah dan tidak tercapainya berat tubuh pedet yang ideal. Dari hasil simulasi diperoleh konstanta anabolisme sebesar 0,3854 dan konstanta katabolisme sebesar 0,0438. Dengan konstanta tersebut, diperoleh rata-rata kesalahan absolut sebesar 4,9708% (6,5358 kg). Selanjutnya, diperoleh hasil bahwa sapi dapat dikawinkan pada saat memiliki berat tubuh 273,9152 kg sampai 303,2340 kg dengan umur 59-66 minggu (14-16 bulan).
Temas de investigación relevantes en ciencias administrativas: una mirada desde el análisis de redes
Authors: Del Río-Cortina Abel Aníbal , Amador-Moncada Jorge Armando , Rentería-Ramos Rafael Ricardo , Urrea-Ospina Iván Darío 
Published: December 2021

Existen fuertes críticas a la baja relación que existe entre los procesos de investigación y el contexto empresarial en las facultades, los programas de administración y las escuelas de negocios poco posicionados. Este documento tiene como objetivo determinar los temas relevantes en ciencias administrativas mediante la aplicación de análisis de redes para identificar las instituciones que manejan temas recurrentes, aquellas que abordan temas especializados y los territorios con investigación en el campo administrativo. Se identificaron las mejores facultades, programas de administración y escuelas de negocios a nivel mundial y nacional y a partir de esta selección, se construyó un modelo relacional de las interacciones entre las instituciones y las temáticas utilizando la teoría de redes. Se implementó este tipo de análisis dadoque permite la revisión sistemática y sus resultados se convierten en insumos que contribuyen a la posterior toma de decisiones por parte de las instituciones inmersas en este campo. Entre los resultados se encuentran como nodos institucionales notables la Escuela de Negocios de Columbia, la Escuela de Negocios Insead, la Universidad de Negocios Cornell, la Escuela de Negocios de Harvard y, finalmente, la Escuela de Gestión SLOAN del Massachusetts Institute of Technology. Finalmente, se identificaron como nodos temáticos relevantes aquellos relacionados con finanzas, emprendimiento, globalización y liderazgo.
Laboratory Investigation of Impact of Slickwater Composition on Multiphase Permeability Evolution in Tight Sandstones
Authors: Abaa Kelvin , Wang John , Elsworth Derek , Ityokumbul Mku 
Published: December 2021

Summary
Fracturing fluid filtrate that leaks off during injection is imbibed by strong capillary forces present in low-permeability sandstones and may severely reduce the effective gas permeability during cleanup and post-fracture production. This work aims to investigate the role fracturing fluid filtrate from slickwater has on rock-fluid and fluid-fluid interactions and to quantify the resulting multiphase permeability evolution during imbibition and drainage of the filtrate by means of specialized core laboratory techniques. Three suites of experiments were conducted. In the first suite of experiments, a fluid leakoff test was conducted on selected core samples to determine the extent of polymer invasion and leakoff characteristics. In the second suite, multigas relative permeability measurements were conducted on sandstone plugs saturated with fracturing fluid filtrate. A combination of controlled fluid evaporation and pulse decay permeability technique was used to measure liquid and gas effective permeabilities for both drainage and imbibition cycles. These experiments aim to capture dynamic permeability evolution during invasion and cleanup of fracturing fluid (slickwater). The final suite of experiments consists of adsorption flow tests to investigate, identify, and quantify possible mechanisms for adsorption of the polymeric molecules of friction reducers present in the fluid filtrate to the pore walls of the rock sample. Imbibition tests and observations of contact angles were conducted to validate possible wettability changes.
Results from multiphase permeability flow tests show an irreversible reduction in endpoint brine permeability and relative permeability with increasing concentration of friction reducer. Our results also show that effective gas permeability during drainage/cleanup of the imbibed slickwater fluid is controlled to a large degree by trapped gas saturation than by changes in interfacial tension. Adsorption flow tests identified adsorption of polymeric molecules of the friction reducer present in the fluid to the pore walls of the rock. The adsorption friction reducer increases the wettability of the rock surface and results in the reduction of liquid relative permeability. The originality of this work is to diagnose formation damage mechanisms from laboratory experiments that adequately capture multiphase permeability evolution specific to a slickwater fluid system, during imbibition and cleanup. This will be useful in optimizing fracturing fluid selection.
The Nuclear Factor-Kappa B Signaling Pathway is Involved in Transient Receptor Potential Vanilloid 4-Mediated Inflammatory Responses and Neuronal Death in Mice with Pilocarpine-Induced Status Epilepticus
Authors: An Dong , Qi Xiuting , Li Kunpeng , Xu Weixing , Wang Yue , Chen Xi , Sha Sha , Wu Chunfeng , Du Yimei , Chen L 
Published: December 2021

Abstract
The blockage of transient receptor potential vanilloid 4 (TRPV4) greatly reduces hippocampal neuronal injury in mice with temporal lobe epilepsy through inhibiting inflammation. NF-κB signaling pathway is activated during epilepsy, leading to enhanced inflammation and neuronal injury. Here, we explored whether TRPV4 blockage could affect the NF-κB pathway in mice with pilocarpine-induced status epilepticus (PISE). Application of a TRPV4 antagonist markedly attenuated the PISE-induced increase in hippocampal HMGB1, TLR4, phospho (p)-IκK (p-IκK), and p-IκBα protein levels, as well as those of cytoplasmic p-NF-κB p65 (p-p65) and nuclear NF-κB p65 and p50; in contrast, the application of GSK1016790A, a TRPV4 agonist, showed similar changes to PISE mice. Administration of the TLR4 antagonist TAK-242 or the NF-κB pathway inhibitor BAY 11-7082 led to a noticeable reduction in the hippocampal protein levels of cleaved IL-1β, IL-6 and TNF, as well as those of cytoplasmic p-p65 and nuclear p65 and p50 in GSK1016790A-injected mice. Finally, administration of either TAK-242 or BAY 11-7082 greatly increased neuronal survival in hippocampal CA1 and CA2/3 regions in GSK1016790A-injected mice. We conclude that TRPV4 activation increases HMGB1 and TLR4 expression, leading to IκK and IκBα phosphorylation and, consequently, NF-κB activation and nuclear translocation. The resulting increase in pro-inflammatory cytokine production is responsible for TRPV4 activation-induced neuronal injury. Meanwhile, blocking TRPV4 can downregulate HMGB1/TLR4/IκK/κBα/NF-κB signaling following PISE onset, an effect that may underlie the neuroprotective ability of TRPV4 blockage in mice with PISE.
S-Ketamine As An Adjuvant in Patient-controlled Intravenous Analgesia for Prevention of Postpartum Depression: A Randomized Controlled Trial
Authors: Han Yaqian , Li Pule , Miao Mengrong , Tao Yuan , Xia Kang , Zhang Jiaqiang 
Published: December 2021

Abstract
(1) Background: Postpartum depression (PPD) is a common complication after cesarean section. Recent studies have shown that intravenous S-ketamine during surgery could be effective in preventing PPD. However, no evidence shows S-ketamine used in patient-controlled intravenous analgesia (PCIA) could reduce the incidence of PPD. Our aim was to explore the effect of S-ketamine as an adjuvant in patient-controlled intravenous analgesia (PCIA) for prevention of postpartum depression. (2) Methods: In a single center, 375 parturients scheduled to undergo cesarean section were recruited and randomly assigned to two groups: Control group (C group) or S-ketamine group (S group). Parturients in both groups received PCIA, the formula of Group C: sufentanil 2 μg/kg + tropisetron 10 mg, S group was the same as C group except 0.5mg/kg S-ketamine. The primary outcome was the incidence of PPD measured by the Edinburgh Postnatal Depression Scale (EPDS) after surgery. The secondary outcomes were EPDS scores, Visual analogue scale (VAS) scores, and Ramsay Sedation Scale (RSS) scores, and incidence of related adverse events. (3) Results: A total of 275 puerpera were finally included and randomly assigned to two groups. The incidence of depression at postoperative 3, 14, 28days in C group and S group were 17.6%vs8.2%, P<0.05;24.2%vs9.8%, P<0.05, and 19.0%vs 17.2%, P=0.76, respectively). As for EPDS scores, C group and S group at 3,14, and 28 days after cesarean were 7.65±3.14vs6.00±2.47, p<0.05,7.62±3.14vs6.38±2.67, p<0.05, and 7.35±3.17vs6.90±2.78, p=0.15, respectively. Adverse events such as headache and nausea were 3.3% vs 4.1%, p=0.755; 5.9%vs8.2%, p=0.481. (4) Conclusions: 0.01mg/kg/h S-ketamine as an adjuvant in patient-controlled intravenous analgesia can significantly reduce the incidence of postpartum depression within 14 days and relieve postoperative pain within 48h after cesarean section, without increasing the incidence of adverse reactions.Trial registration: ChiCTR, ChiCTR2100050263，retrospectively registered. Registered 24/08/2021.
Prescreening For Osteoporosis With Forearm Bone Densitometry In Health Examination Population
Authors: Yue Chun , Ding Na , Xu Lu-Lu , Fu Ya-Qian , Guo Yuan-Wei , Yang Yan-Yi , Zhao Xian-Mei , Sheng Zhi-Feng 
Published: December 2021

Abstract
Background: Early detection and timely prophylaxis can retard the progression of osteoporosis. The purpose of this study was to determine the usefulness of peripheral Dual Energy X-ray Absorptiometry(DXA) for osteoporosis screening. We utilized AKDX-09W-I, a domestic brand instrument to perform peripheral DXA. Briefly, we acquired bone mineral density (BMD) data from manufacturer-supplied density-gradient phantoms and 30 volunteers to investigate its accuracy and precision, then we measured BMD from 150 volunteers using both AKDX (left forearm) and Hologic Discovery Wi (left forearm, left hip and L1 - L4 vertebrae) simultaneously. Correlation analysis of BMD results from two instruments was assessed by simple linear regression, the Receiver Operating Characteristic (ROC) curves and Areas Under the Curves (AUCs) were evaluated for the diagnostic value of left forearm BMD from AKDX in detecting osteoporosis.Results: In vitro precision errors of AKDX BMD were 0.40%, 0.20%, 0.19%, respectively, on low-, medium-, and high-density phantom; in vivo precision was 1.65%. Positive correlation was observed between AKDX BMD and Hologic BMD at the forearm (r = 0.670), L1–L4 (r = 0.430, femoral neck (r = 0.449), and total hip (r = 0.559). With Hologic DXA T-score as the gold standard, the sensitivity of AKDX T-score < -1 for identifying suboptimal bone health was 63.0% and 76.1%, respectively, at the distal one-third radius and at any site, and the specificity was 73.9% and 90.0%, respectively; the AUCs were 0.708 and 0.879. The sensitivity of AKDX T-score ≤ -2.5 for identifying osteoporosis at the distal one-third radius and at any site was 76.9% and70.4%, respectively, and the specificity was 80.4% and 78.0%, respectively; the AUCs were 0.823 and 0.778.Conclusions: Peripheral DXA appears to be a reliable tool for prescreening for osteoporosis.
What lies underneath: Precise classification of brain states using time-dependent topological structure of dynamics
Authors: Soler-Toscano Fernando , Galadí Javier , Escrichs Anira , Perl Yonatan , López-González Ane , Sitt Jacobo , Annen Jitka , Gosseries Olivia , Thibaut Aurore , Panda Rajanikant , Esteban Francisco J. , Laureys Steven , Kringelbach Morten , Langa Jose , Deco Gustavo 
Published: December 2021

Abstract
The self-organising global dynamics underlying brain states emerge from complex recursive nonlinear interactions between interconnected brain regions. Until now, all efforts of capturing the causal mechanistic generating principles have proven elusive, since they have been unable to describe the non-stationarity of brain dynamics, i.e. time-dependent changes. Here, we present a novel framework able to characterise brain states with high specificity, precisely by modelling the time-dependent dynamics. Through describing the topological structure of the brain at each moment in time (its ‘information structure’), we are able to classify different brain states by using the statistics across time of these exact ‘information structures’ hitherto hidden in the neuroimaging dynamics. Proving the strong potential of this framework, we were able to classify the neuroimaging data from two classes of comatose patients (minimally conscious state and unresponsive wakefulness syndrome) compared with healthy controls with very high precision.
MicroRNA-155 Expression With Brucella Infection in vitro and in vivo and Decreased Serum Levels of MicroRNA-155 in Patients With Brucellosis
Authors: Zhang Xi , Chen Jingjing , Cheng Huimin , Zhu Jinying , Dong Qiao , Zhang Huan , Chen Zeliang 
Published: December 2021

Abstract
Infection by Brucella is characterized by the inhibition of host immune responses. MicroRNA-155 (miR-155) has been implicated in the immune response to many diseases. In this study, miR-155 expression during Brucella 16M infection of macrophages and mice were analyzed. Expression of miR-155 was significantly induced in macrophages at 24 hours post infection. Analysis of infected mice showed that miR-155 was inhibited at 7 and 14 days, but induced at 28 days. Very interestingly, the induction or inhibition trend was reversed at 7 and 14 days in 16M△virB-infected mice. This suggested that decreased expression of miR-155 at an early stage of infection was dependent on intracellular replication. In humans with brucellosis, serum levels of miR-155 were significantly decreased compared to those without brucellosis and healthy volunteers. Significant correlations were observed between serum level of miR-155 and serum anti-Brucella antibody titers and symptom of sweat. The decrease in miR-155 with Brucella infection contrasts with the increase in miR-155 observed in Mycobacterium tuberculosis infection. This contrasting effect suggests that Brucella interferes with miR-155-regulated immune responses through a unique mechanism. Taken together, data from this study indicate that Brucella infection affects miR-155 expression, and that human brucellosis patients show decreased serum levels of miR-155.
Towards a structurally resolved human protein interaction network
Authors: Elofsson Arne , Burke David , Bryant Patrick , Barrio-Hernandez Inigo , Memon Danish , Pozzati Gabriele , Shenoy Aditi , Zhu Wensi , Dunham Alistair , Albanese Pascal , Keller Andrew , Scheltema Richard , Bruce James , Leitner Alexander , Kundrotas Petras , Beltrao Pedro 
Published: December 2021

Abstract
All cellular functions are governed by complex molecular machines that assemble through protein-protein interactions. Their atomic details are critical to the study of their molecular mechanisms but fewer than 5% of hundreds of thousands of human interactions have been structurally characterized. Here, we test the potential and limitations of recent progress in deep-learning methods using AlphaFold2 to predict structures for 65,484 human interactions. We show that higher confidence models are enriched in interactions supported by affinity or structure based methods and can be orthogonally confirmed by spatial constraints defined by cross-link data. We identify 3,137 high confidence models, of which 1,371 have no homology to a known structure, from which we identify interface residues harbouring disease mutations, suggesting potential mechanisms for pathogenic variants. We find groups of interface phosphorylation sites that show patterns of co-regulation across conditions, suggestive of coordinated tuning of multiple interactions as signalling responses. Finally, we provide examples of how the predicted binary complexes can be used to build larger assemblies. Accurate prediction of protein complexes promises to greatly expand our understanding of the atomic details of human cell biology in health and disease.
Response of Sorghum (Sorghum Bicolor L. Moench) To Potassium, Zinc, and Boron Fertilizers in Wag-Lasta, Northern, Ethiopia
Authors: Esubalew Tilahun , Sebnie Workat 
Published: December 2021

Abstract
Background

In Ethiopia using fertilizer started in the early 1960. But it still depended solely on urea and DAP. Today, according to ATA and Ministry of Agriculture and Natural Resources K, Zn, B, S, and Cu are in deficit in Ethiopia and Amhara Region as well as in Wag-Lasta areas. But some studies conducted in Wag-Lasta and throughout the region in different crops indicate that these nutrients had no response on crop yields. So, this experiment was conducted in 2017 rain feed cropping season on five farmers’ parcels per location to verify the response of sorghum to potassium, zinc, and boron fertilizers.
Methods

The treatments were: NPS, NPSK, NPSZnB, and NPSZnBK, with the recommended rate of 46 and 23 kg ha −1 N and P2O5 respectively for Sekota, 23 kg ha−1 N, and P2O5 for Lasta. Moreover, 150, 1.47, and 0.07 kg ha−1 KCl, Zn, and B respectively used uniformly for both locations. The experiment was laid out in randomized complete block design (RCBD) with three replications. The collected data were analyzed by SAS software version 9.0.
Results

Analysis of variance revealed that the application of potassium, zinc, and boron had no significant effect (P ≤0.05) on sorghum yield and yield components at all sites. The exchangeable potassium content in the soil of the study sites is high and very high. The result disagrees with the soil fertility map which showed more than 98% of potassium deficiency and more than 80% NPSZnB deficiency. Hence, the application of K, Zn, and B fertilizers had no yield advantage over the recommended N and P fertilizers in the Wag-Lasta areas.
Conclusions

Therefore, currently to increase production and productivity of crops in Wag-Lasta areas using of recommended rate of nitrogen and phosphorous with organic fertilizer sources for each district is the best option rather than using K, Zn, and B fertilizers.
Ca2+ Participates in Programmed Cell Death by Modulating ROS During Pollen Cryopreservation
Authors: Ren Ruifen , Zhou Hao , Zhang Lingling , Jiang Xueru , Liu Yan 
Published: December 2021

Abstract
Programmed cell death (PCD) is one of the reasons for the decline in pollen viability after cryopreservation. However, the role of calcium ions (Ca2+) in PCD during pollen cryopreservation has not been revealed in the existing studies. In this study, Paeonia lactiflora 'Fen Yu Nu' pollen was used as the research material for investigating the effects of Ca2+ changes on PCD indices and reactive oxygen species (ROS) during pollen cryopreservation. The results showed that after cryopreservation, with the decrease of pollen viability, the Ca2+ content significantly increased. The regulation of Ca2+ content had a significant effect on PCD indices, which showed that the Ca2+ carrier A23187 accelerated the decrease of mitochondrial membrane potential level and increased the activity of caspase-3-like and caspase-9-like proteases and the apoptosis rate. The expression levels of partial pro-PCD genes were upregulated, the anti-PCD gene BI-1 was downregulated, and the addition of Ca2+ chelating agent EGTA had the opposite effect. The addition of the Ca2+ carrier A23187 after cryopreservation significantly increased the ROS content of pollen, the addition of the Ca2+ chelating agent EGTA had the opposite effect, and Ca2+ regulators also had significant effects on the contents of ROS production and clearance-related substances. Ca2+ affected intracellular ROS content by acting on the ROS production and clearance system during the cryopreservation of pollen and is thus involved in the occurrence of PCD.