bluuebunny/crossref_metadata_2025_split huggingface dataset:
Follow-up and symptom persistence after esophageal food impaction
Authors: Rosenwald Kent , Pan Zhaoxing , Andrews Rachel , Menard-Katcher Calies 
Published: May 2021

AbstractEsophageal food impactions (EFI) are associated with esophageal pathology, most commonly eosinophilic esophagitis (EoE). Obtaining biopsies provides opportunity for diagnosis, which is important since treatment of EoE decreases the risk for future EFI. Outpatient follow-up rates remain suboptimal and outcomes of patients without timely follow-up are unknown. We aimed to identify the factors associated with pediatric subspecialty follow-up post-EFI and to determine the symptom burden in patients without follow-up. We performed a retrospective review of patients presenting with EFI at a tertiary children’s hospital between 2010 and 2018. Patients without subspecialty follow-up within 1 year of EFI were included in a prospective telephone survey investigating the barriers to care, outcomes, and symptoms. Clinical characteristics were compared between groups. Multivariate analysis was used to control for multiple variables. There were 127 EFI identified in 123 individuals (73% male, mean age: 12.2 years). Esophageal biopsies were collected in 76% of cases, and 49% of patients had follow-up. Individuals with follow-up were more likely (P ≤ 0.05) to have had biopsies. In a multivariate analysis, written recommendation for follow-up (Odds Ratio: 6.9 [2.4–19.5], P = 0.001) as well as atopic history and identified stricture were associated with a higher likelihood of follow-up. Those without follow-up had subsequent stricture (35%), dilation (44%), or EFI (39%), and 55% (12/22) described ongoing esophageal symptoms. Identification of treatable findings at time of EFI and ongoing symptom burden after EFI support an imperative for follow-up after EFI. Clear recommendations are a modifiable factor that may improve follow-up in this population.
Percutaneous management strategies for STEMI with coexisting aneurysmal coronary- to-pulmonary artery fistula: a case report
Authors: Frampton Jennifer , Rothstein Eric , Iribarne Alexander , Powell Richard , Young Michael N 
Published: May 2021

Abstract

Background
Coexistence of coronary artery fistulas and atherosclerotic coronary artery disease (CAD) is rare.

Case summary
We present a unique case of a patient initially presenting with an anterior ST-elevation myocardial infarction, subsequently found to have two-vessel CAD and an aneurysmal left coronary-to-right pulmonary artery fistula.

Discussion
After discussion with the patient and a multidisciplinary discussion with the heart team, consisting of cardiovascular surgery, interventional cardiology, and vascular surgery, a percutaneous approach was chosen. He underwent successful multivessel percutaneous coronary intervention followed by fistula embolization.
Modified double guiding catheter ‘Ping Pong’ technique to treat large coronary perforation: a case report
Authors: Gupta Himanshu , Kaur Navjyot , Sharma Yashpaul , Lim Soo Teik 
Published: May 2021

Abstract

Background
Despite improvement in available tools and techniques, procedural complications like coronary perforation can occur during percutaneous coronary intervention (PCI). Severe proximal coronary perforations are usually caused by balloon and vessel size mismatch but can also occur with appropriately sized balloons or stents if the coronary vessel has very eccentric calcification or if there is negative remodelling of the vessel.

Case summary
A 74-year-old man with a history of type II diabetes mellitus, hypertension, and chronic coronary syndrome (previous PCI 10 years before) presented with unstable angina of 2 weeks of duration. Coronary angiography revealed a patent stent in left anterior descending artery, significant disease in left circumflex artery and diffuse calcified lesion in dominant right coronary artery (RCA). During angioplasty of RCA, the patient developed severe Ellis grade III perforation, which was successfully managed with modified double guiding catheter ‘Ping Pong’ technique. In this technique, the already engaged 7 French (F) Amplatz Left 1 guide catheter was used to deliver the bulky covered stent in highly tortuous and calcified RCA while a second 6F guide catheter (Judkin Right) introduced through contralateral femoral access was used for introducing the balloon, which initially sealed the perforation and subsequently acted as a distal anchor to provide strong support to deliver the covered stent.

Conclusion
In a case of severe coronary perforation, modified Ping Pong technique using a small-sized second guide catheter complimentary to the first guide catheter, can be used to deploy bulky covered stent.
Staged endovascular treatment of aneurysmal progressive development of an intercostal arteries island reimplanted with the loop-graft technique
Authors: Bossi Matteo , De Freitas Dhaniel Morgado , Chiesa Roberto , Kahlberg Andrea 
Published: May 2021

Abstract
A staged endovascular strategy was used to treat the aneurysmal evolution of the aortic island including intercostal arteries reimplanted in a loop-graft, following thoraco-abdominal aortic open repair in a young patient diagnosed with Marfan syndrome. First, selective coil embolization of patent intercostal arteries was performed in 2 separate sessions, to minimize the risk of spinal cord ischaemia. Then, the aneurysm was successfully excluded occluding the loop graft with 2 PTFE-membrane vascular plugs.
Endocardial/endothelial angiocrines regulate cardiomyocyte development and maturation and induce features of ventricular non-compaction
Authors: Rhee Siyeon , Paik David T , Yang Johnson Y , Nagelberg Danielle , Williams Ian , Tian Lei , Roth Robert , Chandy Mark , Ban Jiyeon , Belbachir Nadjet , Kim Seokho , Zhang Hao , Phansalkar Ragini , Wong Ka Man , King Devin A , Valdez Caroline , Winn Virginia D , Morrison Ashby J , Wu Joseph C , Red-Horse Kristy 
Published: May 2021

Abstract

Aims
Non-compaction cardiomyopathy is a devastating genetic disease caused by insufficient consolidation of ventricular wall muscle that can result in inadequate cardiac performance. Despite being the third most common cardiomyopathy, the mechanisms underlying the disease, including the cell types involved, are poorly understood. We have previously shown that endothelial cell-specific deletion of the chromatin remodeller gene Ino80 results in defective coronary vessel development that leads to ventricular non-compaction in embryonic mouse hearts. We aimed to identify candidate angiocrines expressed by endocardial and endothelial cells (ECs) in wildtype and LVNC conditions in Tie2Cre;Ino80 fl/fltransgenic embryonic mouse hearts, and test the effect of these candidates on cardiomyocyte proliferation and maturation.

Methods and results
We used single-cell RNA-sequencing to characterize endothelial and endocardial defects in Ino80-deficient hearts. We observed a pathological endocardial cell population in the non-compacted hearts and identified multiple dysregulated angiocrine factors that dramatically affected cardiomyocyte behaviour. We identified Col15a1 as a coronary vessel-secreted angiocrine factor, downregulated by Ino80-deficiency, that functioned to promote cardiomyocyte proliferation. Furthermore, mutant endocardial and endothelial cells up-regulated expression of secreted factors, such as Tgfbi, Igfbp3, Isg15, and Adm, which decreased cardiomyocyte proliferation and increased maturation.

Conclusions
These findings support a model where coronary endothelial cells normally promote myocardial compaction through secreted factors, but that endocardial and endothelial cells can secrete factors that contribute to non-compaction under pathological conditions.
Comparative phylogenetics of repetitive elements in a diverse order of flowering plants (Brassicales)
Authors: Beric Aleksandra , Mabry Makenzie E , Harkess Alex E , Brose Julia , Schranz M Eric , Conant Gavin C , Edger Patrick P , Meyers Blake C , Pires J Chris 
Published: May 2021

Abstract
Genome sizes of plants have long piqued the interest of researchers due to the vast differences among organisms. However, the mechanisms that drive size differences have yet to be fully understood. Two important contributing factors to genome size are expansions of repetitive elements, such as transposable elements (TEs), and whole-genome duplications (WGD). Although studies have found correlations between genome size and both TE abundance and polyploidy, these studies typically test for these patterns within a genus or species. The plant order Brassicales provides an excellent system to further test if genome size evolution patterns are consistent across larger time scales, as there are numerous WGDs. This order is also home to one of the smallest plant genomes, Arabidopsis thaliana—chosen as the model plant system for this reason—as well as to species with very large genomes. With new methods that allow for TE characterization from low-coverage genome shotgun data and 71 taxa across the Brassicales, we confirm the correlation between genome size and TE content, however, we are unable to reconstruct phylogenetic relationships and do not detect any shift in TE abundance associated with WGD.
A genetic screen for regulators of muscle morphogenesis inDrosophila
Authors: Ou Tiffany , Huang Gary , Wilson Beth , Gontarz Paul , Skeath James B , Johnson Aaron N 
Published: May 2021

AbstractThe mechanisms that determine the final topology of skeletal muscles remain largely unknown. We have been developing Drosophila body wall musculature as a model to identify and characterize the pathways that control muscle size, shape, and orientation during embryogenesis. Our working model argues muscle morphogenesis is regulated by (1) extracellular guidance cues that direct muscle cells toward muscle attachment sites, and (2) contact-dependent interactions between muscles and tendon cells. While we have identified several pathways that regulate muscle morphogenesis, our understanding is far from complete. Here, we report the results of a recent EMS-based forward genetic screen that identified a myriad of loci not previously associated with muscle morphogenesis. We recovered new alleles of known muscle morphogenesis genes, including back seat driver, kon-tiki, thisbe, and tumbleweed, arguing our screen had the depth and precision to uncover myogenic genes. We also identified new alleles of spalt-major, barren, and patched that presumably disrupt independent muscle morphogenesis pathways. Equally as important, our screen shows that at least 11 morphogenetic loci remain to be mapped and characterized. Our screen has developed exciting new tools to study muscle morphogenesis, which may provide future insights into the mechanisms that regulate skeletal muscle topology.
Plasma C-Terminal Agrin Fragment as an Early Biomarker for Sarcopenia: Results From the GenoFit Study
Authors: Pratt Jedd , De Vito Giuseppe , Narici Marco , Segurado Ricardo , Pessanha Ludmilla , Dolan Jackie , Conroy Judith , Boreham Colin 
Published: May 2021

Abstract
Barriers associated with direct muscle quantification have prevented a consistent implementation of therapeutic measures for sarcopenia. Recently, the relevance of circulating C-terminal agrin fragment (CAF) as an accessible screening method alternative for sarcopenia has gained credence. Accordingly, this study aimed to verify the pertinence of plasma CAF as a biomarker for sarcopenia. Three hundred healthy adults aged between 50 and 83 years took part in this study. Sarcopenia was diagnosed according to the European Working Group on Sarcopenia in Older People criteria. Body composition was assessed using dual-energy x-ray absorptiometry, while muscle strength was examined using hand dynamometry. Plasma CAF concentrations were determined using a commercially available ELISA kit. CAF concentrations were significantly associated with appendicular lean mass (ALM), but not grip strength (p = .028, p = .575, respectively). Plasma CAF concentrations were significantly elevated in sarcopenic individuals compared to nonsarcopenic (p &lt; .001). Overall, individuals with low grip strength or low ALM displayed significantly higher CAF levels compared to healthy controls, after adjusting for age and body mass index (p = .027, p = .003, respectively). In males, those with low grip strength or low ALM had significantly elevated CAF levels (p = .039, p = .027, respectively), while in females, only those with low ALM had significantly raised CAF concentrations, compared to healthy controls (p = .035). Our findings illuminate the potential relevance of CAF as an accessible biomarker for skeletal muscle health. CAF determination may enhance clinical practice by facilitating more widespread treatment strategies for sarcopenia. Nevertheless, future research is needed to confirm the diagnostic pertinence of CAF concentrations in screening for sarcopenia.
Impact of Cytomegalovirus and Age on T-Cell Subsets Defined by CD161, CD300a, and/or CD57 Expression in Healthy Andalusians
Authors: Formentini Martina , Navas Ana , Hassouneh Fakhri , Lopez-Sejas Nelson , Alonso Corona , Tarazona Raquel , Solana Rafael , Pera Alejandra 
Published: May 2021

AbstractImmunosenescence affects innate and adaptive immunity impairing the response to pathogens and vaccines. Chronic infection with cytomegalovirus (CMV) has been shown to drive “early immunosenescence” and can considerably affect both the function and phenotype of immune cells, especially T cells. We have previously shown that the expression of CD57, CD300a, and CD161 was differentially affected by age and chronic CMV infection, indicating that these markers are a hallmark of CMV infection and T-cell aging. The aim of this present study was to clarify whether these 3 markers define distinct T-cell subpopulations with a specific functional and molecular signature. Specifically, we analyzed the effect of age and chronic CMV infection on the functionality of T cells according to CD161, CD300a, and CD57 expression. We found that these markers defined different T-cell subsets, both at the phenotypic and functional levels. CD57 was the best biomarker for CD4+ T-cell cytotoxicity and was a hallmark of CMV infection. CD300a+ T cells were heterogeneous and included different cell subsets. The population of CD161+ T cells dramatically decreased with age, independently of CMV infection, and represented a sign of age-associated immune system alterations. The latter could contribute to an increased risk of autoimmune disease and infection in older adults. Our results underline the importance of better understanding the factors involved in the immunosenescence process to be able to uncover new biomarkers and open new avenues for the investigation and development of novel age-related disease therapies.
Dynamic Modeling, Analysis, and Design Synthesis of a Reduced Complexity Quadruped with a Serpentine Robotic Tail
Authors: Liu Yujiong , Ben-Tzvi Pinhas 
Published: May 2021

Synopsis
Serpentine tail structures are widely observed in the animal kingdom and are thought to help animals to handle various motion tasks. Developing serpentine robotic tails and using them on legged robots has been an attractive idea for robotics. This article presents the theoretical analysis for such a robotic system that consists of a reduced complexity quadruped and a serpentine robotic tail. Dynamic model and motion controller are formulated first. Simulations are then conducted to analyze the tail’s performance on the airborne righting and maneuvering tasks of the quadruped. Using the established simulation environment, systematic analyses on critical design parameters, namely, the tail mounting point, tail length, torso center of mass (COM) location, tail–torso mass ratio, and the power consumption distribution, are performed. The results show that the tail length and the mass ratio influence the maneuvering angle the most while the COM location affects the landing stability the most. Based on these design guidelines, for the current robot design, the optimal tail parameters are determined as a length of two times as long as the torso length and a weight of 0.09 times as heavy as the torso weight.
Acclimation to Future Climate Exposes Vulnerability to Cold Extremes in Intertidal Sea Hares
Authors: McAlpine-Bellis Elizabeth , Stillman Jonathon H , Tanner Richelle L 
Published: May 2021

Synopsis
Highly dynamic environments like estuaries will undergo unpredictable shifts in thermal and salinity regimes with ongoing climate change. These interactive stressors fluctuate predictably and seasonally over historical periods, which has facilitated the evolution of wide environmental tolerance in some estuarine inhabitants. However, physiological and behavioral acclimatization is seasonally based for many estuarine species, meaning that a shift in the unpredictability of climate events and trends will disrupt the effectiveness of evolved tolerance mechanisms. Of particular concern are extreme cold events and high-volume precipitation events, which will acutely and unpredictably alter an estuarine habitat. The eelgrass sea hare, Phyllaplysia taylori, has documented euryhaline and eurythermal tolerance to summer conditions, but the winter environment may pose a greater challenge to seasonally relevant acclimatization scenarios. Here, we characterized lower critical thermal limits, and behavioral responses to stimuli leading up to these limits, in two central California P. taylori populations under four temperature–salinity scenarios in a laboratory acclimation experiment. Acclimation to warmer conditions significantly increased critical thermal minima, while fresher conditions resulted in high mortality. However, the surviving individuals in the fresher conditions were able to respond to stimuli more quickly overall, despite their shortest response time being at a higher temperature than the saltier-acclimated individuals. Within the environmental context of their natural habitats, we find that acclimation to climate change-induced warming will hinder sea hares’ ability to weather existing and future cold extremes and precipitation events.
Baseline Asymptomatic Malaria Infection and Immunogenicity of Recombinant Vesicular Stomatitis Virus–Zaire Ebola Virus Envelope Glycoprotein
Authors: Mahon Barbara E , Simon Jakub , Widdowson Marc-Alain , Samai Mohamed , Rogier Eric , Legardy-Williams Jennifer , Liu Kenneth , Schiffer Jarad , Lange James , DeByle Carolynn , Pinner Robert , Schuchat Anne , Slutsker Laurence , Goldstein Susan 
Published: May 2021

Abstract

Background
The effect of malaria infection on the immunogenicity of the recombinant vesicular stomatitis virus–Zaire Ebola virus envelope glycoprotein (GP) vaccine (rVSVΔG-ZEBOV-GP) (ERVEBO) is unknown.

Methods
The Sierra Leone Trial to Introduce a Vaccine Against Ebola (STRIVE) vaccinated 7998 asymptomatic adults with rVSVΔG-ZEBOV-GP during the 2014–2016 Ebola epidemic. In STRIVE’s immunogenicity substudy, participants provided blood samples at baseline and at 1, 6, and 9–12 months. Anti-GP binding and neutralizing antibodies were measured using validated assays. Baseline samples were tested for malaria parasites by polymerase chain reaction.

Results
Overall, 506 participants enrolled in the immunogenicity substudy and had ≥1 postvaccination antibody titer. Of 499 participants with a result, baseline malaria parasitemia was detected in 73 (14.6%). All GP enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization test (PRNT) geometric mean titers (GMTs) at 1, 6, and 9–12 months were above baseline, and 94.1% of participants showed seroresponse by GP-ELISA (≥2-fold rise and ≥200 ELISA units/mL), while 81.5% showed seroresponse by PRNT (≥4-fold rise) at ≥1 postvaccination assessment. In participants with baseline malaria parasitemia, the PRNT seroresponse proportion was lower, while PRNT GMTs and GP-ELISA seroresponse and GMTs showed a trend toward lower responses at 6 and 9–12 months.

Conclusion
Asymptomatic adults with or without malaria parasitemia had robust immune responses to rVSVΔG-ZEBOV-GP, persisting for 9–12 months. Responses in those with malaria parasitemia were somewhat lower.
High Cost and Resource Utilization of Frostbite Readmissions in the United States
Authors: Endorf Frederick W , Nygaard Rachel M 
Published: May 2021

Abstract
Frostbite is a high morbidity, high-cost injury that can lead to digit or limb necrosis requiring amputation. Our primary aim is to describe the rate of readmission following frostbite injury. Our secondary aims are to describe the overall burden of care, cost, and characteristics of repeat hospitalizations of frostbite-injured people. Hospitalizations following frostbite injury (index and readmissions) were identified in the 2016 and 2017 Nationwide Readmission Database. Multivariable logistic regression was clustered by hospital and additionally adjusted for severe frostbite injury, gender, year, payor group, severity, and comorbidity index. Population estimates were calculated and adjusted for by using survey weight, sampling clusters, and stratum. In the 2-year cohort, 1065 index hospitalizations resulted in 1907 total hospitalizations following frostbite injury. Most patients were male (80.3%), lived in metropolitan/urban areas (82.3%), and nearly half were insured with Medicaid (46.4%). Of the 842 readmissions, 53.7% were associated with complications typically associated with frostbite injury. Overall, 29% of frostbite injuries resulted in at least one amputation. The average total cost and total length of stay of readmissions were $236,872 and 34.7 days. Drug or alcohol abuse, homelessness, Medicaid insurance, and discharge against medical advice (AMA) were independent predictors of unplanned readmission. Factors associated with multiple readmissions include discharge AMA and Medicare insurance, but not drug or alcohol abuse or homelessness. The population-based estimated unplanned readmission rate following frostbite injury was 35.4% (95% confidence interval 32.2%–38.6%). This is the first study examining readmissions following frostbite injury on a national level. Drug or alcohol abuse, homelessness, Medicaid insurance, and discharge AMA were independent predictors of unplanned readmission, while only AMA discharge and Medicare insurance were associated with multiple readmissions. Supportive resources (community and hospital-based) may reduce unplanned readmissions of frostbite-injured patients with those additional risk factors.
Social Class Shapes Donation Allocation Preferences
Authors: Vieites Yan , Goldszmidt Rafael , Andrade Eduardo B 
Published: May 2021

Abstract
When considering a charitable act, consumers must often decide on how to allocate their resources across a multitude of possible causes. This article assesses how the relative “urgency” of the causes under consideration (i.e., how critical to human survival the causes are) shapes preferences for specific causes among higher and lower social class consumers. Across a series of studies in a highly unequal socioeconomic environment (Rio de Janeiro, Brazil), we demonstrate that lower-class consumers prefer to donate to urgent causes (e.g., alleviating hunger) compared to non-urgent causes (e.g., encouraging cultural activities), whereas the effect reverses among higher-class consumers. Contrasting experiences with scarcity across social classes vary the consumers’ intrinsic sympathy toward people’s unmet basic needs, which in turn shapes donation allocation preferences. Consistent with this theoretical rationale, class differences in charitable allocations decrease when (a) vivid contextual cues induce sympathy among both higher- and lower-class consumers or (b) the experience with scarcity is similar across social classes. Thus, although class differences in preferences for specific causes can be shifted with relative ease, our findings suggest that those who have the most to give do not spontaneously prioritize what is most urgently needed in society.