bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
Authors: Li Lian-Di , Naveed Muhammad , Du Zi-Wei , Ding Huachen , Gu Kai , Wei Lu-Lu , Zhou Ya-Ping , Meng Fan , Wang Chun , Han Feng , Zhou Qi-Gang , Zhang Jing 
Published: August 2021

AbstractWhether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD.
A study protocol of the photo-supported conversations about the well-being intervention (Be Well™) for people with stress related disorders
Authors: Gunnarsson A. Birgitta , Wagman Petra , Sternudd Hans T. , Holmberg Sara , Holmgren Kristina , Hörberg Ulrica 
Published: August 2021

Abstract
Background
Stress-related illnesses constitute a huge problem in society. The primary care services in Sweden form the first line of care whose role is to coordinate interventions for reducing symptoms, as well as health-promoting interventions. There is lack of knowledge concerning health-promoting interventions for these illnesses. The aim of this study is to evaluate whether photo-supported conversations about well-being (Be Well™) as an intervention, in addition to care as usual within the primary care services, improves health and well-being for patients with stress-related illnesses. The intervention will be compared to a control group, who receive care as usual. A further aim is to conduct a process evaluation.

Methods/Design
This ongoing project has a quasi-experimental design, using quantitative and qualitative methods, and includes patients from primary care centres in two Swedish counties. Seventy patients, 20–67 years, with stress-related illnesses will be recruited. They constitute an intervention group, which receive the intervention together with care as usual, and a control group, which receive care as usual. The intervention, photo-supported conversations about well-being, involves 12 sessions. Care as usual entails medication, occupational therapy, physiotherapy and/or psychotherapy. Data collection is carried out at baseline, and outcomes are assessed directly after the intervention, as well as six months after completion of the intervention. The outcomes are evaluated based on factors related to health, well-being and everyday occupations. Furthermore, data concerning experiences of well-being and perceptions of the intervention will be collected in interviews. The therapists will also be interviewed about their experiences of performing the intervention. Data will be analysed with non-parametric statistics, and qualitative methodology.

Discussion
The project is based on the concept that focusing on well-being despite living with stress-related illness may positively impact health and well-being as well as activity-related aspects, and that photo-supported conversations about well-being can contribute a complement to other treatment and rehabilitation. A strength is the use of a wide range of methods: such as quantitative measures, photographs, and qualitative interviews with participants and therapists. The results will thus provide knowledge about potential effects of this health-promoting intervention.
Trial registration Clinical Trials.gov: NCT04832295; retrospectively registered 2nd April 2021 https://clinicaltrials.gov/ct2/show/NCT04832295
The effect of 12 weeks of mechanical vibration on root resorption: a micro-CT study
Authors: Yilmaz Hakan , Ozlu Fethiye Cakmak , Turk Tamer , Darendeliler Mehmet Ali 
Published: August 2021

AbstractObjectiveThe aim was to investigate the effect of mechanical vibration on root resorption with or without orthodontic force application.Material and methodsTwenty patients who required maxillary premolar extractions as part of orthodontic treatment were randomly divided into two groups of 10: no-force group and force group. Using a split-mouth procedure, each patient’s maxillary first premolar teeth were randomly assigned as either vibration or control side for both groups. A buccally directed vibration of 50 Hz, with an Oral-B HummingBird device, was applied to the maxillary first premolar for 10 min/day for 12 weeks. After the force application period, the maxillary first premolars were extracted and scanned with micro-computed tomography. Fiji (ImageJ), performing slice-by-slice quantitative volumetric measurements, was used for resorption crater calculation. Total crater volumes were compared with the Wilcoxon and Mann–Whitney U tests.ResultsThe total crater volumes in the force and no-force groups were 0.476 mm3and 0.017 mm3on the vibration side and 0.462 mm3and 0.031 mm3on the control side, respectively. There was no statistical difference between the vibration and control sides (P> 0.05). There was more resorption by volume in the force group when compared to the no-force group (P< 0.05).ConclusionMechanical vibration did not have a beneficial effect on reducing root resorption; however, force application caused significant root resorption.
A novel time efficient learning-based approach for smart intrusion detection system
Authors: Seth Sugandh , Singh Gurvinder , Kaur Chahal Kuljit 
Published: August 2021

Abstract
Background
The ever increasing sophistication of intrusion approaches has led to the dire necessity for developing Intrusion Detection Systems with optimal efficacy. However, existing Intrusion Detection Systems have been developed using outdated attack datasets, with more focus on prediction accuracy and less on prediction latency. The smart Intrusion Detection System framework evolution looks forward to designing and deploying security systems that use various parameters for analyzing current and dynamic traffic trends and are highly time-efficient in predicting intrusions.

Aims
This paper proposes a novel approach for a time-efficient and smart Intrusion Detection System.

Method
Herein, we propose a Hybrid Feature Selection approach that aims to reduce the prediction latency without affecting attack prediction performance by lowering the model's complexity. Light Gradient Boosting Machine (LightGBM), a fast gradient boosting framework, is used to build the model on the latest CIC-IDS 2018 dataset.

Results
The proposed feature selection reduces the prediction latency ranging from 44.52% to 2.25% and the model building time ranging from 52.68% to 17.94% in various algorithms on the CIC-IDS 2018 dataset. The proposed model with hybrid feature selection and LightGBM gives 97.73% accuracy, 96% sensitivity, 99.3% precision rate, and comparatively low prediction latency. The proposed model successfully achieved a raise of 1.5% in accuracy rate and 3% precision rate over the existing model. An in-depth analysis of network parameters is also performed, which gives a deep insight into the variation of network parameters during the benign and malicious sessions.
Missing values compensation in duplicates detection using hot deck method
Authors: Ali Abdulrazzak , Emran Nurul A. , Asmai Siti A. 
Published: August 2021

AbstractDuplicate record is a common problem within data sets especially in huge volume databases. The accuracy of duplicate detection determines the efficiency of duplicate removal process. However, duplicate detection has become more challenging due to the presence of missing values within the records where during the clustering and matching process, missing values can cause records deemed similar to be inserted into the wrong group, hence, leading to undetected duplicates. In this paper, duplicate detection improvement was proposed despite the presence of missing values within a data set through Duplicate Detection within the Incomplete Data set (DDID) method. The missing values were hypothetically added to the key attributes of three data sets under study, using an arbitrary pattern to simulate both complete and incomplete data sets. The results were analyzed, then, the performance of duplicate detection was evaluated by using the Hot Deck method to compensate for the missing values in the key attributes. It was hypothesized that by using Hot Deck, duplicate detection performance would be improved. Furthermore, the DDID performance was compared to an early duplicate detection method namely DuDe, in terms of its accuracy and speed. The findings yielded that even though the data sets were incomplete, DDID was able to offer a better accuracy and faster duplicate detection as compared to DuDe. The results of this study offer insights into constraints of duplicate detection within incomplete data sets.
The Ichikado CT score as a prognostic tool for coronavirus disease 2019 pneumonia: a retrospective cohort study
Authors: Araiza Alan , Duran Melanie , Patiño Cesar , Marik Paul E. , Varon Joseph 
Published: August 2021

Abstract
Background
The relationship between computed tomography (CT) and prognosis of patients with COVID-19 pneumonia remains unclear. We hypothesized that the Ichikado CT score, obtained in the first 24 h of hospital admission, is an independent predictor for all-cause mortality during hospitalization in patients with COVID-19 pneumonia.

Methods
Single-center retrospective cohort study of patients with confirmed COVID-19 pneumonia admitted at our institution between March 20th, 2020 and October 31st, 2020. Patients were enrolled if, within 24 h of admission, a chest CT scan, an arterial blood gas, a complete blood count, and a basic metabolic panel were performed. Two independent radiologists, who were blinded to clinical data, retrospectively evaluated the chest CT scans following a previously described qualitative and quantitative CT scoring system. The primary outcome was all-cause in-hospital mortality or survival to hospital discharge. Secondary outcomes were new requirements for invasive mechanical ventilation and hospital length of stay. Cox regression models were used to test the association between potential independent predictors and all-cause mortality.

Results
Two hundred thirty-five patients, 197 survivors and 38 nonsurvivors, were studied. The median Ichikado CT score for nonsurvivors was significantly higher than survivors (P < 0.001). An Ichikado CT score of more than 172 enabled prediction of mortality, with a sensitivity of 84.2% and a specificity of 79.7%. Multivariate analysis identified Ichikado CT score (HR, 7.772; 95% CI, 3.164–19.095; P < 0.001), together with age (HR, 1.030; 95% CI, 1.030–1.060; P = 0.043), as independent predictors of all-cause in-hospital mortality.

Conclusions
Ichikado CT score is an independent predictor of both requiring invasive mechanical ventilation and all-cause mortality in patients hospitalized with COVID-19 pneumonia. Further prospective evaluation is necessary to confirm these findings.
Trial registration: The WCG institutional review board approved this retrospective study and patient consent was waived due to its non-interventional nature (Identifier: 20210799).
Inflammatory markers assessment in an animal model of intracranial hypertension: a randomized trial
Authors: do Espírito Santo Marcelo Prudente , Faria Caroline Silvério , Solla Davi Jorge Fontoura , Pipek Leonardo Zumerkorn , Belon Alessandro Rodrigo , Jeng Brasil Ping , de Andrade Almir Ferreira , Teixeira Manoel Jacobsen , Paiva Wellingson Silva 
Published: August 2021

AbstractBackgroundIntracranial hypertension (ICH) is a common final pathway of most neurosurgical pathologies and leads to poor prognosis if not detected and treated properly. Inflammatory markers have been assessed in clinical scenarios of neurological injuries, in which systemic and brain tissue aggressions may introduce bias. There is a lack of studies under controlled settings to isolate the ICH effect on inflammation. This study aims to evaluate the effects of ICH on the serum concentration of cytokines as biomarkers of neuroinflammation in an experimental model which isolates ICH from potential confounding variables.MethodsAn established model of ICH using an intracerebral pediatric bladder catheter and a multisensor intraparenchymal catheter was used in adult pigs (Sus domesticus). The animals were randomly allocated to 2 groups based on the catheter balloon volume used to simulate the ICP increase (4 ml or 7 ml). Cytokines were measured in 4 timepoints during the experiment: (1) 15 min before balloon insufflation; (2) 5 min after insufflation; (3) 125 min after insufflation; (4) 60 min after deflation. The following cytokines were measured IL-1α; IL-1β; IL–1ra; IL-2; IL-4; IL-6; IL-8; IL-10; IL-12; IL-18; TNFα. Generalized estimating equations were modeled to compare the ICP and cytokines values between the groups along the experiment. The study sample size was powered to detect interactions between the groups and the study moments with an effect size (f) of at least 0.3. The ARRIVE checklist was followed.ResultsA total of 20 animals were studied (10 in each group, 4 ml or 7 ml balloon volume insufflation). The animal model was successful in increasing the ICP along the moments of the experiment (p < 0,001) and in creating an ICP gradient between the groups (p = 0,004). The interaction term (moment × group) was also significant (p < 0,001). There was a significant association between ICP elevation and most cytokines variation. The cytokines IL-1α, IL-1β, IL1-ra, IL-6, IL-12, and IL-18 increased, whereas IL-2, IL-4, and TNF-α decreased. IL-10 did not vary significantly in response to the ICP elevation.ConclusionThe serum concentration of cytokines varied in response to intracranial hypertension. The study demonstrated the specific changes in each cytokine after intracranial hypertension and provides key information to guide neuroinflammation clinical research. The proposed experiment was successful as an animal model to the study of neuroinflammation biomarkers
Meeting report of the fourth annual Tri-Service Microbiome Consortium symposium
Authors: Goodson Michael S. , Barbato Robyn A. , Karl J. Philip , Indest Karl , Kelley-Loughnane Nancy , Kokoska Robert , Mauzy Camilla , Racicot Kenneth , Varaljay Vanessa , Soares Jason 
Published: August 2021

AbstractThe Tri-Service Microbiome Consortium (TSMC) was founded to enhance collaboration, coordination, and communication of microbiome research among U.S. Department of Defense (DoD) organizations. The annual TSMC symposium is designed to enable information sharing between DoD scientists and leaders in the field of microbiome science, thereby keeping DoD consortium members informed of the latest advances within the microbiome community and facilitating the development of new collaborative research opportunities. The 2020 annual symposium was held virtually on 24–25 September 2020. Presentations and discussions centered on microbiome-related topics within four broad thematic areas: (1) Enabling Technologies; (2) Microbiome for Health and Performance; (3) Environmental Microbiome; and (4) Microbiome Analysis and Discovery. This report summarizes the presentations and outcomes of the 4th annual TSMC symposium.
Integration, cultural preservation and transnationalism through state supported immigrant organizations: a study of Sweden’s national ethnic associations
Authors: Frödin Olle , Fredholm Axel , Sandberg Johan 
Published: August 2021

AbstractThis paper explores the activities of Swedish state supported ethnic associations (most of which are immigrant organizations), thus shedding empirical light on how immigrants organize with the help of state support, both nationally and transnationally, in a welfare state context. The paper is based on a study of annual reports of 52 state supported national associations, representing more than a thousand (1046) local immigrant organizations, as well as 17 interviews with representatives of the said organizations. The findings indicate that the welfare state did not crowd out civil societal integration-promoting initiatives, but the state supported immigrant organizations came to occupy a niche in which they primarily offered complementary services with the aim of helping members to navigate the public welfare system. Neither did state support directly shape the content and direction of the political activities of the said organizations. However, the state support seems to have generated welfare channeling effects, in that more immigrant organizations came into existence than would have been the case in the absence of grants. The paper comes down in favor of the thesis that state support in some circumstances can promote political incorporation through immigrant organizations (with a few caveats). Finally, the paper proposes a hypothetical mechanism,homeland-oriented integration,for political incorporation through immigrant organization.
One-pot and one-step automated radio-synthesis of [18F]AlF-FAPI-74 using a multi purpose synthesizer: a proof-of-concept experiment
Authors: Naka Sadahiro , Watabe Tadashi , Lindner Thomas , Cardinale Jens , Kurimoto Kenta , Moore Melissa , Tatsumi Mitsuaki , Mori Yuriko , Shimosegawa Eku , Valla Frank , Kato Hiroki , Giesel Frederik L. 
Published: August 2021

Abstract
Background
Fibroblast activation protein (FAP) is overexpressed in the stroma of many types of cancer. [18F]AlF-FAPI-74 is a positron emission tomography tracer with high selectivity for FAP, which has already shown high accumulation within human tumors in clinical studies. However, [18F]AlF-FAPI-74 radiosynthesis has not been optimized using an automated synthesizer. Herein, we report a one-pot and one-step automated radiosynthesis method using a multi purpose synthesizer.

Results
Radiosynthesis of [18F]AlF-FAPI-74 was performed using a cassette-type multi purpose synthesizer CFN-MPS200. After the recovery rate of trapped [18F]fluoride onto the anion-exchange cartridge using a small amount of eluent was investigated manually, a dedicated [18F]AlF-FAPI-74 synthesis cassette and synthesis program for one-pot and one-step fluorination was developed. The solutions for the formulation of [18F]AlF-FAPI-74 synthesized using this were evaluated to obtain stable radiochemical purity. The recovery rate of [18F]fluoride with only 300 µL of eluent ranged 90 ± 9% by introduction from the male side and elution from the female side of the cartridge. In automated synthesis, the eluted [18F]fluoride and precursor solution containing aluminum chloride were mixed; then, fluorination was performed in a one-pot and one-step process at room temperature for 5 min, followed by 15 min at 95 °C. As a result, the radioactivity of [18F]AlF-FAPI-74 was 11.3 ± 1.1 GBq at the end of synthesis from 32 to 40 GBq of [18F]fluoride, and its radiochemical yield was 37 ± 4% (n = 10). The radiochemical purity at the end of the synthesis was ≥ 97% for all formulation solutions. When the diluent was saline, the radiochemical purity markedly decreased after 4 h of synthesis. In contrast, with phosphate-buffered saline (pH 7.4) or 10 mM phosphate-buffered saline (pH 6.7) containing 100 mg of sodium ascorbate, the radiochemical purity was stable at 97%. Non-radioactive AlF-FAPI-74 and total impurities, including non-radioactive AlF-FAPI-74, were 0.3 ± 0.1 µg/mL and 2.8 ± 0.6 µg/mL. Ethanol concentration and residual DMSO were 5.5 ± 0.2% and 21 ± 6 ppm, respectively.

Conclusions
We established a one-pot one-step automated synthesis method using a CFN-MPS200 synthesizer that provided high radioactivity and stable radiochemical purity for possible clinical applications.
18F-labeled tracers targeting fibroblast activation protein
Authors: Lindner Thomas , Altmann Annette , Giesel Frederik , Kratochwil Clemens , Kleist Christian , Krämer Susanne , Mier Walter , Cardinale Jens , Kauczor Hans-Ulrich , Jäger Dirk , Debus Jürgen , Haberkorn Uwe 
Published: August 2021

Abstract
Background
Cancer-associated fibroblasts are found in the stroma of epithelial tumors. They are characterized by overexpression of the fibroblast activation protein (FAP), a serine protease which was already proven as attractive target for chelator-based theranostics. Unfortunately, the value of gallium-68 labeled tracers is limited by their batch size and the short nuclide half-life. To overcome this drawback, radiolabeling with aluminum fluoride complexes and 6-fluoronicotinamide derivatives of the longer-lived nuclide fluorine-18 was established. The novel compounds were tested for their FAP-specific binding affinity. Uptake and binding competition were studied in vitro using FAP expressing HT-1080 cells. HEK cells transfected with the closely related dipeptidyl peptidase-4 (HEK-CD26) were used as negative control. Small animal positron emission tomography imaging and biodistribution experiments were performed in HT-1080-FAP xenografted nude mice. [18F]AlF-FAPI-74 was selected for PET/CT imaging in a non-small cell lung cancer (NSCLC) patient.

Results
In vitro, 18F-labeled FAPI-derivatives demonstrated high affinity (EC50 = < 1 nm to 4.2 nm) and binding of up to 80% to the FAP-expressing HT1080 cells while no binding to HEK-CD26 cells was observed. While small animal PET imaging revealed unfavorable biliary excretion of most of the 18F-labeled compounds, the NOTA bearing compounds [18F]AlF-FAPI-74 and -75 achieved good tumor-to-background ratios, as a result of their preferred renal excretion. These two compounds showed the highest tumor accumulation in PET imaging. The organ distribution values of [18F]AlF-FAPI-74 were in accordance with the small animal PET imaging results. Due to its less complex synthesis, fast clearance and low background values, [18F]AlF-FAPI-74 was chosen for clinical imaging. PET/CT of a patient with metastasized non-small cell lung cancer (NSCLC), enabled visualization of the primary tumor and its metastases at the hepatic portal and in several bones. This was accompanied by a rapid clearance from the blood pool and low background in healthy organs.

Conclusion
[18F]AlF-labeled FAPI derivatives represent powerful tracers for PET. Owing to an excellent performance in PET imaging, FAPI-74 can be regarded as a promising precursor for [18F]AlF-based FAP-imaging.