bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomarker and Isotopic Composition of Seep Carbonates Record Environmental Conditions in Two Arctic Methane Seeps
Authors: Yao Haoyi , Panieri Giuliana , Lehmann Moritz F. , Himmler Tobias , Niemann Helge 
Published: February 2021

Present-day activity of cold seeps in the ocean is evident from direct observations of methane emanating from the seafloor, the presence of chemosynthetic organisms, or the quantification of high gas concentrations in sediment pore waters and the water column. Verifying past cold seep activity and biogeochemical characteristics is more challenging but may be reconstructed from proxy records of authigenic seep carbonates. Here, we investigated the lipid-biomarker inventory, carbonate mineralogy, and stable carbon and oxygen isotope compositions of seep-associated carbonates from two active Arctic methane seeps, located to the northwest (Vestnesa Ridge; ∼1,200 m water depth) and south (Storfjordrenna; ∼380 m water depth) offshore Svalbard. The aragonite-dominated mineralogy of all but one carbonate sample indicate precipitation close to the seafloor in an environment characterized by high rates of sulfate-dependent anaerobic oxidation of methane (AOM). In contrast, Mg-calcite rich nodules sampled in sediments of Storfjordrenna appear to have formed at the sulfate-methane-transition zone deeper within the sediment at lower rates of AOM. AOM activity at the time of carbonate precipitation is indicated by the 13C-depleted isotope signature of the carbonates [−20 to −30‰ Vienna Pee Dee Belemnite (VPDB)], as well as high concentrations of 13C-depleted lipid biomarkers diagnostic for anaerobic methanotrophic archaea (archaeol and sn2-hydroxyarchaeol) and sulfate-reducing bacteria (iso and anteiso-C15:0 fatty acids) in the carbonates. We also found 13C-depleted lipid biomarkers (diploptene and a 4α-methyl sterol) that are diagnostic for bacteria mediating aerobic oxidation of methane (MOx). This suggests that the spatial separation between AOM and MOx zones was relatively narrow at the time of carbonate formation, as is typical for high methane-flux regimes. The seep-associated carbonates also displayed relatively high δ18O values (4.5–5‰ VPDB), indicating the presence of 18O-enriched fluids during precipitation, possibly derived from destabilized methane gas hydrates. Based on the combined isotopic evidence, we suggest that all the seep carbonates resulted from the anaerobic oxidation of methane during intense methane seepage. The seepage likely was associated to gas hydrates destabilization, which led to the methane ebullition from the seafloor into the water column.
Modelling the Effect of Electrification on Volcanic Ash Aggregation
Authors: Pollastri Stefano , Rossi Eduardo , Bonadonna Costanza , Merrison Jonathan P. 
Published: February 2021

The fine ash released into the atmosphere (particles &lt;63 μm) during explosive volcanic eruptions represents a significant threat for both the ecosystem and many sectors of society. In order to mitigate the associated impact, ash dispersal models need to accurately estimate ash concentration through time and space. Since most fine ash sediments in the form of aggregates, ash dispersal models require a quantitative description of ash aggregation. The physical and chemical processes involved in the collision and sticking of volcanic ash have been extensively studied in the last few decades. Among the different factors affecting volcanic particle aggregation (e.g., turbulence, particle-particle adhesion, presence of liquid and solid water), the charge carried by volcanic particles has been found to play a crucial role. However, Coulomb interactions are not yet taken into account in existing models. In order to fill this gap, we propose a strategy to take charge into account. In particular, we introduce a quantitative model for aggregation of oppositely charged micron—to millimetre-sized objects settling in still air. Our results show that the presence of charge considerably enhances the collision efficiency when one of the colliding objects is very small (&lt;20 µm), and that the sticking efficiency is not affected by particle charge if colliding objects are either small enough (&lt;20 µm) or large enough (&gt;200 µm). Besides providing a theoretical framework to quantify the effect of charge, our findings demonstrate that aggregation models that do not account for electrification significantly underestimate the amount of fine ash that sediments in the form of aggregates, leading to an overestimation of the residence time of fine ash in the atmosphere after explosive volcanic eruptions.
A Knowledge-Based Smart Trainer System for Transferring Knowledge From Coaches to Long Jump Students
Authors: Kamnardsiri Teerawat , Khuwuthyakorn Pattaraporn , Boripuntakul Sirinun , Janchai Worawit 
Published: February 2021

Background:The constructive and specific feedback in guiding long jump athletes to improve their performance in each phase is part of the critical process for achieving desired long jump distance. However, to date, the potential approach for assisting a coach in capturing long jump movement and transferring their knowledge to long jump students is not well-established.Objectives:To investigate the performance of long jump students and evaluate transferring knowledge from coaches to long jump students using a Knowledge-Based Smart Trainer (KBST) System.Methods:Twenty-two participants (fifteen males, mean age = 15.33 ± 1.95 years; seven females, mean age = 14.57 ± 2.07 years) participated in the study. All participants were recruited from eleven sports schools in Thailand. Each participant was instructed to perform the long jump movement, including running, take-off, and landing, for three attempts (Test 1,Test 2,andTest 3).Test 1was the conventional approach (coaches provided the feedback based on their experience).Test 2andTest 3were the KBST system approach (coaches provided the feedback based on the results from KBST system). Two cameras were used to record the participant movement from the starting position to the landing position. The capture data were analyzed by KBST system program. The outcome measures were starting position, maximum velocity, maximum velocity position, and take-off angle. Repeated-Measures ANOVA was conducted to compare the long jump performance across the three trials. The statistical significance was set atp-value &lt; 0.05.Results:There was a statistically significant difference betweenTest 1andTest 3for long jump distance (mean difference = 0.292; Std. Error = 0.129; Sig. = 0.34). However, the mean of take-off angle was similar across the three trials (Test 1 =12.16°,Test 2= 12.71°, andTest 3= 12.95°, respectively).Conclusion:TheKBSTsystem was effective in improving long jump students’ performance and also transferring knowledge from the coach to long jump students.
Preparation and Electrochemical Performance of V2O5 @N-CNT/S Composite Cathode Materials
Authors: Liu Cheng , Xiang Meng , Zhang Haiyang , Feng Shuaiqiang , Xiao Jianrong , Ma Songshan , Li Xinyu 
Published: February 2021

Lithium–sulfur battery hasreceived widespread attention because of its high energy density, low cost, environmental friendliness, and nontoxicity. However, the insulating properties of elemental sulfur, huge volume changes, and dissolution of polysulfides in electrolytes that result in the shuttle effect, low sulfur utilization, and low rate performance seriously hinder the commercialization of lithium–sulfur batteries. In this work, a composite material of nitrogen-doped multiwalled carbon nanotubes and V2O5 was designed and fabricated to serve as the positive electrode of lithium–sulfur battery via the hydrothermal method. The positive electrode of the V2O5@N-CNTs composite material could reach an initial discharge specific capacity of 1,453 mAh g−1at a rate of 0.1C. Moreover, the composite material could maintain a discharge ratio of 538 mAh g−1 at a rate of 0.5C even after 200 charge and discharge cycles. After 400 cycles, the composite had a specific discharge capacity of 439 mAh g−1 at a rate of 1.0C. The excellent electrochemical performance of the V2O5@N-CNT/S composite cathode material was due to the fact that V2O5 contains oxygen ions and has a strong polarized surface. Furthermore, nitrogen doping changed the hybrid structure of carbon atoms and provided additional active sites, thereby improving the conductivity of the material itself and effectively inhibiting the dissolution and diffusion of polysulfides.
Human Mesenchymal Stem Cell-Derived Exosomal microRNA-143 Promotes Apoptosis and Suppresses Cell Growth in Pancreatic Cancer via Target Gene Regulation
Authors: Wang Bingyi , Xu Yan , Wei Yuhua , Lv Lixin , Liu Nanbin , Lin Rui , Wang Xiuyan , Shi Baomin 
Published: February 2021

BackgroundThis study aimed to explore the regulatory mechanism of hsa-miR-143-3p and lncRNA RP11-363N22.3–functioning upstream ofKRAS–in exosomes derived from human mesenchymal stem cells (hMSCs) in pancreatic cancer.MethodsWestern blotting and quantitative PCR were used to determine gene expression.In vitro, cell proliferation, apoptosis, and cell cycle and invasion were evaluated using CCK-8 assay, flow cytometry, and transwell assays, respectively.In vivo, the effect of hsa-miR143-3p was investigated using a tumorigenesis test in nude mice. The association between hsa-miR-143-3p and lncRNA RP11-363N22.3 was investigated using the dual-luciferase assay.Resultshsa-miR-143-3p expression significantly increased in hMSC exosomes than in those in human pancreatic cancer cell line (CFPAC-1) exosomes.In vitro, compared to the MOCK (CFPAC-1 only) group, cell proliferation and invasion were inhibited and apoptosis was induced in the inhibitor NC (CFPAC-1 + MSC-hsa-miR-3p inhibitor NC) group, while these changes were reversed in the inhibitor (CFPAC-1 + MSC-hsa-miR-3p inhibitor) group. The expression of lncRNA RP11-363N22.3 and genes related to miR-143 significantly decreased in the inhibitor NC group compared to the MOCK group, and increased in the inhibitor group compared to inhibitor NC group. A targeted combinatorial effect was observed between lncRNA RP11-363N22.3 and hsa-miR-143-3p.In vivo, the tumor volume of the mimics (CFPAC-1 + MSC-hsa-miR-143-3p mimics) group was smaller than that of the mimics NC (CFPAC-1 + MSC-hsa-miR-143-3p mimics NC) and MOCK groups. H&amp;E staining showed that there were no obvious pathological changes in MOCK and mimic NC groups, while cell necrosis was seen in some regions in mimic groups.Conclusionhsa-miR-143-3p may promote apoptosis and suppress cell growth and invasion in pancreatic cancer.
Mobilized Multipotent Hematopoietic Progenitors Promote Expansion and Survival of Allogeneic Tregs and Protect Against Graft Versus Host Disease
Authors: D’Aveni Maud , Notarantonio Anne-Béatrice , Agbogan Viviane A. , Bertrand Allan , Fouquet Guillemette , Gastineau Pauline , Garfa-Traoré Meriem , De Carvalho Marcelo , Hermine Olivier , Rubio Marie-Thérèse , Zavala Flora 
Published: February 2021

Allogeneic Hematopoietic Stem Cell Transplantation (Allo-HSCT) is routinely performed with peripheral blood stem cells (PBSCs) mobilized by injection of G-CSF, a growth factor which not only modulates normal hematopoiesis but also induces diverse immature regulatory cells. Based on our previous evidence that G-CSF-mobilized multipotent hematopoietic progenitors (MPP) can increase survival and proliferation of natural regulatory T cells (Tregs) in autoimmune disorders, we addressed the question how these cells come into play in mice and humans in an alloimmune setting. Using a C57BL/6 mouse model, we demonstrate that mobilized MPP enhance the immunosuppressant effect exerted by Tregs, against alloreactive T lymphocytes, both in vitro and in vivo. They do so by migrating to sites of allopriming, interacting with donor Tregs and increasing their numbers, thus reducing the lethality of graft-versus-host disease (GVHD). Protection correlates likewise with increased allospecific Treg counts. Furthermore, we provide evidence for a phenotypically similar MPP population in humans, where it shares the capacity to promote selective Treg expansion in vitro. We postulate that G-CSF-mobilized MPPs might become a valuable cellular therapy to expand donor Tregs in vivo and prevent GVHD, thereby making allo-HSCT safer for the treatment of leukemia patients.
Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children
Authors: Badolato-Corrêa Jessica , Carvalho Fabiana Rabe , Paiva Iury Amancio , Familiar-Macedo Débora , Dias Helver Gonçalves , Pauvolid-Corrêa Alex , Fernandes-Santos Caroline , Lima Monique da Rocha Queiroz , Gandini Mariana , Silva Andréa Alice , Baeta Cavalcanti Silvia Maria , de Oliveira Solange Artimos , de Oliveira Vianna Renata Artimos , de Azeredo Elzinandes Leal , Cardoso Claudete Aparecida Araújo , Grifoni Alba , Sette Alessandro , Weiskopf Daniela , de-Oliveira-Pinto Luzia Maria 
Published: February 2021

Background:Zika virus (ZIKV) infection causes for mild and self-limiting disease in healthy adults. In newborns, it can occasionally lead to a spectrum of malformations, the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues, we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants affected by neurological complications or asymptomatic ones.Results:Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools (CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed an increased CD4 and CD8 T-cell responses in mothers compared to children. The degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers, and children, while in CD8 T-cells, low responses were detected in these study groups. The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4 ZIKV MP.Conclusion:Donors with a history of ZIKV infection demonstrated long-term CD4 T cell immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities of CD8 T cells are markedly demonstrated in the early stages of infection, but less detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers' T cells to ZIKV MPs do not appear to be related to their children's clinical outcome. There was also no marked difference in the T cell responses to ZIKV MP between children affected or not with CZS. These data still need to be investigated, including the evaluation of the response of CD8 T cells to other ZIKV peptides.
Influence of Coarse Mg3Bi2 Particles on Deformation Behaviors of Mg-Bi Alloys
Authors: Guo Tingting , Lu Xuerong , Varma Ramesh Kumar , Zhao Cheng , Wang Jun , You Jianwei , Chen Jian 
Published: February 2021

Mg-Bi binary alloys with concentrations of 1, 3, 6, and 9 wt% Bi and ternary Mg-9Bi-2.5Zn alloy were prepared by casting and hot extrusion. The results show that alloying with Bi refined as-cast grains of Mg alloys and the refinement efficiency of Bi is in between Al and Zr. It was also found that a critical value for the area fraction of constituent Mg3Bi2 particles seems to appear, which influences the dynamic recrystallization mechanism during extrusion. This influence results in either uniform or heterogenetic distribution of grain size and different extrusion texture intensities. Furthermore, the addition of Zn affected both the grain size and the area fraction/size of Mg3Bi2. Mechanical results and microstructure/fractography observation suggest that Mg3Bi2 particles do not impact the tensile nor compression yielding stress but act as fracture sources.
Prevalence and Characteristics of Hypoxic Hepatitis in COVID-19 Patients in the Intensive Care Unit: A First Retrospective Study
Authors: Huang Haijun , Li Hong , Chen Shanshan , Zhou Xianlong , Dai Xuan , Wu Jia , Zhang Jun , Shao Lina , Yan Rong , Wang Mingshan , Wang Jiafeng , Tu Yuexing , Ge Minghua 
Published: February 2021

Purpose: Coronavirus disease 2019 (COVID-19) has been associated with acute liver injury in reports worldwide. But no studies to date have described hypoxic hepatitis (HH) in patients with COVID-19. We aim to identify the prevalence of and possible mechanisms of HH in COVID-19 patients in the Intensive Care Unit (ICU).Methods: This retrospective study was conducted on 51 patients with confirmed SARS-CoV-2 infection in the ICU at Zhongnan Hospital of Wuhan University from December 21, 2019, to March 11, 2020. Information on clinical features of enrolled patients was collected for analysis.Results: HH was observed in 5.88% of the ICU patients with SARS-CoV-2 infection. All HH patients were progressing to respiratory failure and peak alanine aminotransferase (ALT) values were 1665, 1414, and 1140 U/L during hospitalization, respectively. All patients with HH died as a result of the deterioration of multiple organ failure (MOF). The dynamic changes of ALT, aspartate transaminase (AST), and total bilirubin (TBIL) levels were more dramatic in HH groups. Levels of TBIL, C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6(IL-6) showed statistically significant elevation in HH cases compared with that in non-HH cases (P &lt; 0.001). Besides, the median survival time of the HH group was significantly shorter than the non-HH group (P &lt; 0.05).Conclusions: In ICU, HH was not a rare condition in patients with severe COVID-19 and has a high mortality. The main causes of HH are respiratory and cardiac failure and may be associated with the immune-mediated inflammatory response. Clinicians should search for any underlying hemodynamic or respiratory instability even in patients with normal ALT levels on admission.
Molecular Analysis of IL-5 Receptor Subunit Alpha as a Possible Pharmacogenetic Biomarker in Asthma
Authors: Elena-Pérez Sandra , Heredero-Jung David Hansoe , García-Sánchez Asunción , Estravís Miguel , Martin Maria J. , Ramos-González Jacinto , Triviño Juan Carlos , Isidoro-García María , Sanz Catalina , Dávila Ignacio 
Published: February 2021

Background: Asthma is a heterogeneous syndrome with a broad clinical spectrum and high drug response variability. The inflammatory response in asthma involves multiple effector cells and mediator molecules. Based on asthma immunopathogenesis, precision medicine can be a promising strategy for identifying biomarkers. Biologic therapies acting on the IL-5/IL-5 receptor axis have been developed. IL-5 promotes proliferation, differentiation and activation of eosinophils by binding to the IL-5 receptor, located on the surface of eosinophils and basophils. This study aimed to investigate the expression of IL5RA in patients with several types of asthma and its expression after treatment with benralizumab, a biologic directed against IL-5 receptor subunit alpha.Methods: Sixty peripheral blood samples, 30 from healthy controls and 30 from asthmatic patients, were selected for a transcriptomic RNAseq study. Differential expression analysis was performed by statistical assessment of fold changes and P-values. A validation study of IL5RA expression was developed using qPCR in 100 controls and 187 asthmatic patients. The effect of benralizumab on IL5RA expression was evaluated in five patients by comparing expression levels between pretreatment and after 3 months of treatment. The IL5RA mRNA levels were normalized to GAPDH and TBP expression values for each sample. Calculations were made by the comparative ΔΔCt method. All procedures followed the MIQE guidelines.Results:IL5RA was one of the most differentially overexpressed coding transcripts in the peripheral blood of asthmatic patients (P = 8.63E-08 and fold change of 2.22). In the qPCR validation study, IL5RA expression levels were significantly higher in asthmatic patients than in controls (P &lt; 0.001). Significant expression differences were present in different asthmatic types. In the biological drug study, patients treated with benralizumab showed a significant decrease in IL5RA expression and blood eosinophil counts. A notable improvement in ACT and lung function was also observed in these patients.Conclusions: These results indicate that IL5RA is overexpressed in patients with different types of asthma. It could help identify which asthmatic patients will respond more efficiently to benralizumab, moving toward a more personalized asthma management. Although further studies are required, IL5RA could play a role as a biomarker and pharmacogenetic factor in asthma.
A New Software for Quantifying Motor Deficit After Stroke: A Case–Control Feasibility Pilot Study
Authors: Gutiérrez Zúñiga Raquel , Alonso de Leciñana María , Díez Alejandro , Torres Iglesias Gabriel , Pascual Alejandro , Higashi Ariaki , Rodríguez Pardo Jorge , Hernández Herrero David , Fuentes Blanca , Díez Tejedor Exuperio 
Published: February 2021

Introduction: The degree of disability after stroke needs to be objectively measured to implement adequate rehabilitation programs. Here, we evaluate the feasibility of a custom-built software to assess motor status after stroke.Methods: This is a prospective, case–control pilot study comparing stroke patients with healthy volunteers. A workout evaluation that included trunk and upper limb movement was captured with Kinect® and kinematic metrics were extracted with Akira®. Trunk and joint angles were analyzed and compared between cases and controls. Patients were evaluated within the first week from stroke onset using the National Institutes of Health Stroke Scale (NIHSS), Fulg-Meyer Assessment (FMA), and modified Rankin Scale (mRS) scales; the relationship with kinematic measurements was explored.Results: Thirty-seven patients and 33 controls were evaluated. Median (IQR) NIHSS of cases was 2 (0–4). The kinematic metrics that showed better discriminatory capacity were body sway during walking (less in cases than in controls, p = 0.01) and the drift in the forearm–trunk angle during shoulder abduction in supination (greater in cases than in controls, p = 0.01). The body sway during walking was moderately correlated with NIHSS score (Rho = −0.39; p = 0.01) but better correlated with mRS score (Rho = −0.52; p &lt; 0.001) and was associated with the absence of disability (mRS 0–1) (OR = 0.64; p = 0.02). The drift in the forearm–trunk angle in supination was associated with the presence of disability (mRS &gt;1) (OR = 1.27; p = 0.04).Conclusion: We present a new software that detects even mild motor impairment in stroke patients underestimated by clinical scales but with an impact on patient functionality.