bluuebunny/crossref_metadata_2025_split huggingface dataset:
Words Matter: An Analysis of the Content and Readability of COVID-19 Information on Clinic Websites
Authors: Sakhuja Mayank , Yelton Brooks , Arent Michelle A. , Noblet Samuel , Macauda Mark M. , Fedrick Delores , Friedman Daniela B. 
Published: September 2021

Objective: To examine content and readability of COVID-19 information on outpatient clinic websites in South Carolina.Participants: Thirty-three outpatient clinic websites.Methods: Using a multi-step search strategy, we located three COVID-19 information content sections from each website. Descriptive statistics were calculated for content section characteristics (focus, information source, target population/race, presence of graphics, mobilizing information). Flesch Reading Ease (FRE), Flesch Kincaid Grade Level (FKGL), and Simple Measure of Gobbledygook (SMOG) were used to calculate reading levels. Mann Whitney U and Kruskal Wallis tests were performed to examine readability levels by clinic type (primary care vs. specialty) and content section characteristics.Results: Twenty-six clinics offered COVID-19 information; 70 content sections across all 26 clinics were analyzed. Sections focused on COVID-19 clinic policies (48.4%), prevention (22.6%), testing (19.4%), and symptoms (9.7%). 93.5% lacked target population, 41.9% provided no information source, 38.7% had no graphics, and none mentioned racial/ethnic groups. MFRE = 54.3, MFKGL = 9.9, MSMOG = 9.5.Conclusion: COVID-19 information focused mainly on clinic policy and was written at a ninth-grade skill level. Findings suggest there is opportunity for clinics to update their online content to convey more plain language and sourced information, especially for high-risk groups.
Heterogeneous Genetic Diversity Estimation of a Promising Domestication Medicinal Motherwort Leonurus Cardiaca Based on Chloroplast Genome Resources
Authors: Sun Jiahui , Wang Yiheng , Garran Thomas Avery , Qiao Ping , Wang Mengli , Yuan Qingjun , Guo Lanping , Huang Luqi 
Published: September 2021

Leonurus cardiaca has a long history of use in western herbal medicine and is applied for the treatment of gynaecological conditions, anxiety, and heart diseases. Because of its botanical relationship to the primary Chinese species, L. japonicus, and extensive medical indications that go beyond the traditional indications for the Chinese species, it is a promising medicinal resource. Therefore, the features of genetic diversity and variability in the species have been prioritized. To explore these issues, we sequenced the chloroplast genomes of 22 accessions of L. cardiaca from different geographical locations worldwide using high-throughput sequencing. The results indicate that L. cardiaca has a typical quadripartite structure and range from 1,51,236 bp to 1,51,831 bp in size, forming eight haplotypes. The genomes all contain 114 distinct genes, including 80 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Comparative analysis showed abundant diversity of single nucleotide polymorphisms (SNPs), indels, simple sequence repeats (SSRs) in 22 accessions. Codon usage showed highly similar results for L. cardiaca species. The phylogenetic and network analysis indicated 22 accessions forming four clades that were partly related to the geographical distribution. In summary, our study highlights the advantage of chloroplast genome with large data sets in intraspecific diversity evaluation and provides a new tool to facilitate medicinal plant conservation and domestication.
Inhibition of IP3R/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways
Authors: Ye Liu , Zeng Qi , Ling Maoyao , Ma Riliang , Chen Haishao , Lin Fei , Li Zhao , Pan Linghui 
Published: September 2021

RationaleDisruption of intracellular calcium (Ca2+) homeostasis is implicated in inflammatory responses. Here we investigated endoplasmic reticulum (ER) Ca2+ efflux through the Inositol 1,4,5-trisphosphate receptor (IP3R) as a potential mechanism of inflammatory pathophysiology in a ventilator-induced lung injury (VILI) mouse model.MethodsC57BL/6 mice were exposed to mechanical ventilation using high tidal volume (HTV). Mice were pretreated with the IP3R agonist carbachol, IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB) or the Ca2+ chelator BAPTA-AM. Lung tissues and bronchoalveolar lavage fluid (BALF) were collected to measure Ca2+ concentrations, inflammatory responses and mRNA/protein expression associated with ER stress, NLRP3 inflammasome activation and inflammation. Analyses were conducted in concert with cultured murine lung cell lines.ResultsLungs from mice subjected to HTV displayed upregulated IP3R expression in ER and mitochondrial-associated-membranes (MAMs), with enhanced formation of MAMs. Moreover, HTV disrupted Ca2+ homeostasis, with increased flux from the ER to the cytoplasm and mitochondria. Administration of carbachol aggravated HTV-induced lung injury and inflammation while pretreatment with 2-APB or BAPTA-AM largely prevented these effects. HTV activated the IRE1α and PERK arms of the ER stress signaling response and induced mitochondrial dysfunction-NLRP3 inflammasome activation in an IP3R-dependent manner. Similarly, disruption of IP3R/Ca2+ in MLE12 and RAW264.7 cells using carbachol lead to inflammatory responses, and stimulated ER stress and mitochondrial dysfunction.ConclusionIncrease in IP3R-mediated Ca2+ release is involved in the inflammatory pathophysiology of VILI via ER stress and mitochondrial dysfunction. Antagonizing IP3R/Ca2+ and/or maintaining Ca2+ homeostasis in lung tissue represents a prospective treatment approach for VILI.
Association Between Alcohol Consumption and Risk of Bladder Cancer: A Dose-Response Meta-Analysis of Prospective Cohort Studies
Authors: Lao Yongfeng , Li Xiaolong , He Lijuan , Guan Xin , Li Rongxin , Wang Yanan , Li Yanyou , Wang Yunchang , Li Xu , Liu Shuai , Dong Zhilong 
Published: September 2021

BackgroundControversial results of the association between alcohol consumption and risk of bladder cancer were reported by the previous meta-analyses.ObjectiveTo quantitatively investigate the association between alcohol consumption and risk of bladder cancer based on prospective cohort studies, and explore whether there is potential dose-response relation.MethodPubMed, EMBASE, the Cochrane Library databases, China Biology Medicine disc (CBM), and Chinese National Knowledge Infrastructure (CNKI) were searched for relevant studies. Categorical meta-analysis was performed for risk estimates of any alcohol consumers versus non-drinkers as well as different drinking degrees (light, moderate, and heavy) versus none. And two-stage generalized least-squares regression and restricted cubic spline, as well as fixed-effects dose-response models, were used for linear and nonlinear dose-response relation exploration.Results9 prospective cohort studies including 1,971,396 individuals were finally included. We did not observe a significant association between alcohol intake and the risk of bladder cancer in the entire population. Linear association was detected in those who consumed alcohol from liquor or spirits (P linear=0.02). One drink increment each day of alcohol could elevate the risk of bladder cancer by 9% (RR=1.09; 95%CI: 1.01-1.17). Alcohol was a risk factor of bladder cancer for male drinkers (RR=1.23; 95%CI: 1.13-1.35; I2=3.7%), while none linear or nonlinear relation was found.ConclusionNo significant association between alcohol consumption and bladder cancer risk was found in the entire population, but there was a linear dose-response relation in those who consume alcohol from liquor or spirits. Alcohol may elevate the risk of bladder cancer in males in a dose-independent way.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, PROSPERO (CRD42020216195).
A Novel Nine-lncRNA Risk Signature Correlates With Immunotherapy in Hepatocellular Carcinoma
Authors: Nie Ye , Li Jianhui , Wu Wenlong , Guo Dongnan , Lei Xinjun , Zhang Tianchen , Wang Yanfang , Mao Zhenzhen , Zhang Xuan , Song Wenjie 
Published: September 2021

BackgroundHepatocellular carcinoma is one of the most common malignant tumors with a very high mortality rate. The emergence of immunotherapy has brought hope for the cure of hepatocellular carcinoma. Only a small number of patients respond to immune checkpoint inhibitors, and ferroptosis and tertiary lymphoid structure contribute to the increased response rate of immune checkpoint inhibitors; thus, we first need to identify those who are sensitive to immunotherapy and then develop different methods to improve sensitivity for different groups.MethodsThe sequencing data of hepatocellular carcinoma from The Cancer Genome Atlas and Gene Expression Omnibus was downloaded to identify the immune-related long non-coding RNAs (lncRNAs). LncRNAs related to survival data were screened out, and a risk signature was established using Cox proportional hazard regression model. R software was used to calculate the riskScore of each patient, and the patients were divided into high- and low-risk groups. The prognostic value of riskScore and its application in clinical chemotherapeutic drugs were confirmed. The relationship between riskScore and immune checkpoint genes, ferroptosis genes, and genes related to tertiary lymphoid structure formation was analyzed by Spearman method. TIMER, CIBERSORT, ssGSEA, and ImmuCellAI were used to evaluate the relative number of lymphocytes in tumor. The Wilcoxon signed-rank test confirmed differences in immunophenoscore between the high- and low-risk groups.ResultsData analysis revealed that our signature could well predict the 1-, 2-, 3-, and 5-year survival rates of hepatocellular carcinoma and to predict susceptible populations with Sorafenib. The risk signature were significantly correlated with immune checkpoint genes, ferroptosis genes, and tertiary lymphoid structure-forming genes, and predicted tumor-infiltrating lymphocyte status. There was a significant difference in IPS scores between the low-risk group and the high-risk group, while the low-risk group had higher scores.ConclusionThe riskScore obtained from an immune-related lncRNA signature could successfully predict the survival time and reflect the efficacy of immune checkpoint inhibitors. More importantly, it is possible to select different treatments for different hepatocellular carcinoma patients that increase the response rate of immune checkpoint inhibitors and will help improve the individualized treatment of hepatocellular carcinoma.
Genetic Variants of CLPP and M1AP Are Associated With Risk of Non-Small Cell Lung Cancer
Authors: Li Xianghan , Zou Yiran , Li Teng , Wong Thomas K. F. , Bushey Ryan T. , Campa Michael J. , Gottlin Elizabeth B. , Liu Hongliang , Wei Qingyi , Rodrigo Allen , Patz Edward F. 
Published: September 2021

BackgroundSingle nucleotide polymorphisms (SNPs) are often associated with distinct phenotypes in cancer. The present study investigated associations of cancer risk and outcomes with SNPs discovered by whole exome sequencing of normal lung tissue DNA of 15 non-small cell lung cancer (NSCLC) patients, 10 early stage and 5 advanced stage.MethodsDNA extracted from normal lung tissue of the 15 NSCLC patients was subjected to whole genome amplification and sequencing and analyzed for the occurrence of SNPs. The association of SNPs with the risk of lung cancer and survival was surveyed using the OncoArray study dataset of 85,716 patients (29,266 cases and 56,450 cancer-free controls) and the Prostate, Lung, Colorectal and Ovarian study subset of 1,175 lung cancer patients.ResultsWe identified 4 SNPs exclusive to the 5 patients with advanced stage NSCLC: rs10420388 and rs10418574 in the CLPP gene, and rs11126435 and rs2021725 in the M1AP gene. The variant alleles G of SNP rs10420388 and A of SNP rs10418574 in the CLPP gene were associated with increased risk of squamous cell carcinoma (OR = 1.07 and 1.07; P = 0.013 and 0.016, respectively). The variant allele T of SNP rs11126435 in the M1AP gene was associated with decreased risk of adenocarcinoma (OR = 0.95; P = 0.027). There was no significant association of these SNPs with the overall survival of lung cancer patients (P &gt; 0.05).ConclusionsSNPs identified in the CLPP and M1AP genes may be useful in risk prediction models for lung cancer. The previously established association of the CLPP gene with cancer progression lends relevance to our findings.
Predicting Response to Systemic Chemotherapy for Advanced Gastric Cancer Using Pre-Treatment Dual-Energy CT Radiomics: A Pilot Study
Authors: Liu Yi-yang , Zhang Huan , Wang Lan , Lin Shu-shen , Lu Hao , Liang He-jun , Liang Pan , Li Jun , Lv Pei-jie , Gao Jian-bo 
Published: September 2021

ObjectiveTo build and assess a pre-treatment dual-energy CT-based clinical-radiomics nomogram for the individualized prediction of clinical response to systemic chemotherapy in advanced gastric cancer (AGC).MethodsA total of 69 pathologically confirmed AGC patients who underwent dual-energy CT before systemic chemotherapy were enrolled from two centers in this retrospective study. Treatment response was determined with follow-up CT according to the RECIST standard. Quantitative radiomics metrics of the primary lesion were extracted from three sets of monochromatic images (40, 70, and 100 keV) at venous phase. Univariate analysis and least absolute shrinkage and selection operator (LASSO) were used to select the most relevant radiomics features. Multivariable logistic regression was performed to establish a clinical model, three monochromatic radiomics models, and a combined multi-energy model. ROC analysis and DeLong test were used to evaluate and compare the predictive performance among models. A clinical-radiomics nomogram was developed; moreover, its discrimination, calibration, and clinical usefulness were assessed.ResultAmong the included patients, 24 responded to the systemic chemotherapy. Clinical stage and the iodine concentration (IC) of the tumor were significant clinical predictors of chemotherapy response (all p &lt; 0.05). The multi-energy radiomics model showed a higher predictive capability (AUC = 0.914) than two monochromatic radiomics models and the clinical model (AUC: 40 keV = 0.747, 70 keV = 0.793, clinical = 0.775); however, the predictive accuracy of the 100-keV model (AUC: 0.881) was not statistically different (p = 0.221). The clinical-radiomics nomogram integrating the multi-energy radiomics signature with IC value and clinical stage showed good calibration and discrimination with an AUC of 0.934. Decision curve analysis proved the clinical usefulness of the nomogram and multi-energy radiomics model.ConclusionThe pre-treatment DECT-based clinical-radiomics nomogram showed good performance in predicting clinical response to systemic chemotherapy in AGC, which may contribute to clinical decision-making and improving patient survival.
The Impact of Dabigatran and Rivaroxaban on Variation of Platelet Activation Biomarkers and DRT Following Percutaneous Left Atrial Appendage Closure
Authors: Li Xiaoye , Zhang Xiaochun , Jin Qinchun , Li Yanli , Zhou Daxin , Lv Qianzhou , Ge Junbo 
Published: September 2021

Background: The current post-procedure antithrombotic recommendation for left atrial appendage closure (LAAC) remains empiric. This study was designed to compare variations in platelet activation biomarkers and device-related thrombosis (DRT) under different antithrombotic regimens following LAAC.Methods: This study enrolled 105 consecutive patients with atrial fibrillation who underwent LAAC successfully and received post-procedure anticoagulation with either dabigatran (N = 33) or rivaroxaban (N = 72). After 3 months of anticoagulation treatment, thromboelastogram was used to evaluate thrombin receptor–activating peptide (TRAP)–induced platelet aggregation (PA). Measurements of platelet activation biomarkers, including thrombin–antithrombin complex (TAT), P-selectin, von Willebrand disease (vWF), and CD40L, were performed immediately before the LAAC procedure and after 3 months of post-procedure anticoagulation. Repeated transesophageal echocardiography was performed to evaluate DRT during follow-ups.Results: Three (4.2%) patients in the rivaroxaban and 4 (12.1%) patients in the dabigatran group experienced DRT events (odds ratio (OR) = 0.315, 95% confidence interval (95%CI): 0.066–1.489, p = 0.129) during follow-ups. The TRAP-induced PA was statistically significantly higher in the dabigatran group (62.9% vs 59.7%, p = 0.028*). Statistically significant increases in plasma concentration of TAT, P-selectin, and vWF were observed after 3 months of exposure to dabigatran when compared with rivaroxaban. An increased expression of platelet activation biomarkers was observed in DRT subjects compared with non–DRT subjects in terms of P-selectin and vWF (65.28 ± 13.93 ng/L vs 32.14 ± 12.11 ng/L, p = 0.037; 501.92 ± 106.48 U/L vs 280.98 ± 54.10 U/L, p = 0.045; respectively). Multivariate regression analysis indicated that the use of dabigatran might be an independent predictor of DRT (p = 0.022; OR = 4.366, 95%CI: 0.434–10.839). Furthermore, the CHA2DS2-VASc score (OR = 2.076, p = 0.016) and CD40L levels (OR = 1.015, p = 0.021) were independent predictors of increased D-dimer levels.Conclusions: Post-LAAC anticoagulation with dabigatran may increase the risk of DRT by enhancing platelet reactivity. In light of this potential increased risk in DRT, the authors recommend against using dabigatran for post-procedural anticoagulation in patients who have undergone LAAC.
Tree Species Composition in Mixed Plantations Influences Plant Growth, Intrinsic Water Use Efficiency and Soil Carbon Stock
Authors: Niccoli Francesco , Danise Tiziana , Innangi Michele , Pelleri Francesco , Manetti Maria Chiara , Mastrolonardo Giovanni , Certini Giacomo , Fioretto Antonietta , Battipaglia Giovanna 
Published: September 2021

Species interactions in mixed plantations can influence tree growth, resources capture and soil fertility of the stands. A combined approach of tree-ring analyses and carbon stable isotope was used to check tree growth and water use efficiency of two species, Populus alba L. and Juglans regia L., intercropped with each other and with N-fixing or competitive production species. Furthermore, soil analyses were performed to understand how the different intercropping systems can influence soil characteristics, in particular soil carbon stock. Dendrochronological data showed that during the first years, the growth of principal species was favored by intercropping. This positive effect decreased in the following years in most of intercropped stands, due to light competition with the crown of companion species. Carbon isotope data showed that P. alba and J. regia had the highest intrinsic water use efficiency when growing with Elaeagnus umbellata Thunb, a shrubby species with a shallow root system that favors a non-competitive exploitation of soil water resources. Finally, the intercropping of the principal species with Corylus avellana L. promoted the highest soil C stock. Our findings confirmed the importance to consider the plantation dynamics and wood formation in the long-run and to apply appropriate thinning and pruning interventions to counteract interspecific competition.
PENERAPAN PENGGUNAAN ALAT PELINDUNG DIRI DI LABORATORIUM TEKNIK GIGI KOTA PEKANBARU
Authors: Natassa Jihan , Ayuningsih Reynha , Syahwanda Wasis 
Published: September 2021

Personal protective equipment is a tool to protect workers from direct danger due to work accidents. Dental engineering services carried out in dental engineering laboratories have become an inseparable part of the efforts to provide dental health services with other related health workers. Work accidents that occur in dental technicians include various incidents such as body pain, stiffness, sore fingers, pierced by wires, irritated eyes and work stress and there are still workers who do not wear personal protective equipment while working. The purpose of this study was to determine the application of the use of personal protective equipment in the Dental Engineering Laboratory of Pekanbaru City in 2020. This research method is qualitative by conducting interviews and observations. This research was conducted at the Smile Dental, Riau Dental and Athallah Dental Laboratories in July-August 2020. The informants in this study were 9 employees of the dental engineering laboratory. The results of this study are most informants have good knowledge about personal protective equipment, good attitude of workers in using personal protective equipment, have never conducted training on personal protective equipment and there is no training, personal protective equipment facilities are already available in the laboratory but are not yet complete. The conclusion of this research is that employees' knowledge and attitude are good regarding personal protective equipment, training has never been carried out by employees and personal protective equipment facilities are available in the laboratory but are not yet complete. It is expected that the laboratory will conduct training and equip personal protective equipment so that employees can always apply the use of personal protective equipment while working.

Bibliography : 8 (1997-2012)
Keywords : Personal Protective Equipment, Laboratory
Tolerance of F6 Red Rice Lines against Iron (Fe) Stress
Authors: Mustikarini Eries D , Prayoga G Ibnu , Santi R , Khodijah Siti , Lestari T 
Published: September 2021

Dryland area in Indonesia reaches 108.8 millions ha (69.4%) of the total dry land with the potential to expand the area of food crops reaching 7.1 million ha, which is dominated by ultisol and oxisol soil types. Plant constraint cultivation on ultisol soil types is the acidic soil pH with high Al and Fe levels. One of the efforts that can be made to cultivate plants on ultisol soil is the selection of plants that are tolerant of Fe stress. This study aimed to determine a tolerance level and F6 red rice lines that were tolerant of iron (Fe) stress in ultisol soils. The experimental design used was factorial Completely Randomized Design (CRD) consisting of 2 factors, the first factor consisted of 12 treatments (10 test lines and 2 comparison varieties) and the second factor was iron stress with concentrations (0 ppm and 551,55 ppm). The stress was applied after 3 WAP. The stress resistance test to iron (Fe) should that all lines of red rice F6 on ultisol soils have a score of 1 which indicates the stress tolerance of Fe at a concentration of 551,55 ppm Fe. The 23A-56-30-25-12 and 23A-56-30-25-13 lines were the best lines that were tolerant of 551,55 ppm iron (Fe) stress with the highest growth and yield. The lines tested can be developed on ultisol soils because they are tolerant of Fe.