bluuebunny/crossref_metadata_2025_split huggingface dataset:
CONTRIBUIÇÕES DE UM PROGRAMA DE MENTORIA PARA O DESENVOLVIMENTO PROFISSIONAL DE PROFESSORAS INICIANTES DO ENSINO FUNDAMENTAL – ANOS INICIAIS
Authors: Pinheiro Tarciana dos Santos , De Souza Ana Paula Gestoso 
Published: July 2021

Esta pesquisa é de caráter qualitativo descritivo-analítico tendo um ambiente natural como fonte direta de dados, estes são descritivos e a maior preocupação se concentra no processo, com a complexidade das trajetórias dos participantes, ou seja, o significado que as pessoas dão às coisas e à sua vida é objeto de atenção do pesquisador. Analisa a trajetória formativa percorrida por duas professoras iniciantes, atuantes no ensino fundamental –anos iniciais, ao se inserirem em um programa de acompanhamento e apoio ao iniciante, intitulado mentoria. No programa em questão um professor experiente (mentor) auxilia professores iniciantes a desenvolver competências profissionais mais rapidamente e na fundamentação de novas práticas profissionais. A partir da análise das trajetórias profissionais dessas duas iniciantes compartilhadas em um ambiente virtual de aprendizagem foi possível delinear tensões e aprendizagens que envolvem o percurso inicial de seu desenvolvimento profissional. A pesquisa evidenciou a importância de uma formação contínua, e, assim, traduz a essencial relevância dos programas de indução, uma vez que percebe-se um dos fatores indispensáveis ao desenvolvimento profissional docente: o acompanhamento desses profissionais no início da carreira ameniza as tensões, condiciona novas perspectivas, providencia quebra de paradigmas, amplia os repertórios teóricos/metodológicos dos(as) professores(as). Assim, o programa de mentoria pode ser visto como uma oportunidade e experiência planejada de forma sistemática com o intuito de propiciar que o iniciante se envolva em um movimento de questionamento das situações que vivencia na prática docente e a construção de planos de ação para superação das tensões.
Surface Mechanism of Fe3+ Ions on the Improvement of Fine Monazite Flotation With Octyl Hydroxamate as the Collector
Authors: Zheng Qingzhu , Qian Yunlou , Zou Dan , Wang Zhen , Bai Yang , Dai Haidong 
Published: July 2021

Froth flotation of fine minerals has always been an important research direction in terms of theory and practice. In this paper, the effect and mechanism of Fe3+ on improving surface hydrophobicity and flotation of fine monazite using sodium octyl hydroxamate (SOH) as a collector were investigated through a series of laboratory tests and detection measurements including microflotation, fluorescence spectrum, zeta potential, and X-ray photoelectron spectroscopy (XPS). Flotation tests have shown that fine monazite particles (−26 + 15 μm) cannot be floated well with the SOH collector compared to the coarse fraction (−74 + 38 μm). However, adding a small amount of Fe3+ to the pulp before SOH can significantly improve the flotation of fine monazite. This is because the addition of Fe3+ promotes the adsorption of SOH and greatly improves the hydrophobicity of the monazite surface. This can result in the formation of a more uniform and dense hydrophobic adsorption layer, as shown by the fluorescence spectrum and zeta potential results. From the XPS results, Fe3+ reacts with surface O atoms on the surface of monazite to form a monazite–Osurf–Fe group that acts as a new additional active site for SOH adsorption. A schematic model was also proposed to explain the mechanism of Fe3+ for improving surface hydrophobicity and flotation of fine monazite using octyl hydroxamate as a collector. The innovative point of this study is using a simple reagent scheme to float fine mineral particles rather than traditional complex processes.
DNA Condensation Triggered by the Synergistic Self-Assembly of Tetraphenylethylene-Viologen Aggregates and CT-DNA
Authors: Saraswathi Sajena Kanangat , Karunakaran Varsha , Maiti Kaustabh Kumar , Joseph Joshy 
Published: July 2021

Development of small organic chromophores as DNA condensing agents, which explore supramolecular interactions and absorbance or fluorescence-based tracking of condensation and gene delivery processes, is in the initial stages. Herein, we report the synthesis and electrostatic/groove binding interaction–directed synergistic self-assembly of the aggregates of two viologen-functionalized tetraphenylethylene (TPE-V) molecules with CT-DNA and subsequent concentration-dependent DNA condensation process. TPE-V molecules differ in their chemical structure according to the number of viologen units. Photophysical and morphological studies have revealed the interaction of the aggregates of TPE-V in Tris buffer with CT-DNA, which transforms the fibrous network structure of CT-DNA to partially condensed beads-on-a-string-like arrangement with TPE-V aggregates as beads via electrostatic and groove binding interactions. Upon further increasing the concentration of TPE-V, the “beads-on-a-string”-type assembly of TPE-V/CT-DNA complex changes to completely condensed compact structures with 40–50 nm in diameter through the effective charge neutralization process. Enhancement in the melting temperature of CT-DNA, quenching of the fluorescence emission of ethidium bromide/CT-DNA complex, and the formation of induced CD signal in the presence of TPE-V molecules support the observed morphological changes and thereby verify the DNA condensation abilities of TPE-V molecules. Decrease in the hydrodynamic size, increase in the zeta potential value with the addition of TPE-V molecules to CT-DNA, failure of TPE-V/cucurbit(8)uril complex to condense CT-DNA, and the enhanced DNA condensation ability of TPE-V2 with two viologen units compared to TPE-V1 with a single viologen unit confirm the importance of positively charged viologen units in the DNA condensation process. Initial cytotoxicity analysis on A549 cancer and WI-38 normal cells revealed that these DNA condensing agents are non-toxic in nature and hence could be utilized in further cellular delivery studies.
Margin-to-Margin Seafloor Spreading in the Eastern Gulf of Aden: A 16 Ma-Long History of Deformation and Magmatism from Seismic Reflection, Gravity and Magnetic Data
Authors: Gillard Morgane , Leroy Sylvie , Cannat Mathilde , Sloan Heather 
Published: July 2021

In this paper we present and analyze spreading-parallel seismic transects that image the oceanic crust in the eastern Gulf of Aden, from the Oman to the Socotra margins, across the active Sheba mid-oceanic ridge and between the Socotra-Hadbeen and Eastern Gulf of Aden Fracture Zones. The correlation of potential field data sets and gravity modelling allow us to document the spreading history of this oceanic basin from the onset of seafloor spreading ∼16 Ma-ago to the present. Two main oceanic sub-domains display distinct structural characteristics associated with different magmatic budgets at this mid-ocean ridge. In addition, we document the occurrence of a magmatic pulse at the Sheba Ridge around 11 Ma leading to the construction of a magmatic plateau in the western part of the studied area. This event led to substantial deformation and additional magmatism in previously formed oceanic crust. It could be related to an off-axis magmatic event previously identified in the adjacent Sheba segment, itself possibly related to the Afar plume.
Preoperatively Predicting the Central Lymph Node Metastasis for Papillary Thyroid Cancer Patients With Hashimoto’s Thyroiditis
Authors: Min Yu , Huang Yizhou , Wei Minjie , Wei Xiaoyuan , Chen Hang , Wang Xing , Chen Jialin , Xiang Ke , Feng Yang , Yin Guobing 
Published: July 2021

BackgroundThe preoperative distinguishment of lymph nodes with reactive hyperplasia or tumor metastasis plays a pivotal role in guiding the surgical extension for papillary thyroid carcinoma (PTC) with Hashimoto’s thyroiditis (HT), especially in terms of the central lymph node (CLN) dissection. We aim to identify the preparative risk factors for CLN metastasis in PTC patients concurrent with HT.Materials and MethodsWe retrospectively reviewed and analyzed the data including the basic information, preoperative sonographic characteristics, and thyroid function of consecutive PTC patients with HT in our medical center between Jan 2019 and Apr 2021. The Chi-square and Fisher’s exact tests were used for comparison of qualitative variables among patients with or without CLN metastasis. Univariate and multivariate logistic regression analyses were used to determine the risk factors for CLN metastasis. The nomogram was constructed and further evaluated by two cohorts produced by 1,000 resampling bootstrap analysis.ResultsA total of 98 in 214 (45.8%) PTC patients were identified with CLN metastasis. In multivariate analysis, four variables including high serum thyroglobulin antibody (TgAb) level (&gt;1,150 IU/ml), lower tumor location, irregular margin of CLN, and micro-calcification in the CLN were determined to be significantly associated with the CLN metastasis in PTC patients with HT. An individualized nomogram was consequently established with a favorable C-index of 0.815 and verified via two internal validation cohorts.ConclusionsOur results indicated that preoperatively sonographic characteristics of the tumor and lymph node condition combined with serum TgAb level can significantly predict the CLN in PTC patients with HT and the novel nomogram may further help surgeons to manage the CLN in this subpopulation.
The Added Value of Whole-Exome Sequencing for Anomalous Fetuses With Detailed Prenatal Ultrasound and Postnatal Phenotype
Authors: He Miao , Du Liu , Xie Hongning , Zhang Lihe , Gu Yujun , Lei Ting , Zheng Ju , Chen Dan 
Published: July 2021

ObjectivesThe objective of the study was to explore the added value of whole-exome sequencing (WES) in abnormal fetuses with detailed prenatal ultrasound and postnatal phenotype with normal karyotype and chromosomal microarray analysis (CMA).MethodsParents of fetuses with structural abnormalities by prenatal ultrasound who consented to provide fetal samples were prospectively recruited from January 2017 to December 2019. With aneuploidies or cases with copy number variations (CNVs) excluded, WES was performed for cases with normal karyotype and CMA results. Detailed prenatal ultrasound and postnatal imaging or pathology features were recommended for further interpretation of genetic variants.ResultsWES was performed for 94 eligible fetuses, DNA samples of which were extracted from 53 parent–fetus trios and 41 proband-only fetal tissues. A diagnostic genetic variant was identified in 37 (39.4%) of 94 fetuses, and 34 (64.2%) were detected in 53 trios, which was significantly greater than 3 (7.3%) in 41 proband-only cases (p &lt; 0.001). In 34 trios with diagnostic genetic variants, 23 (67.6%) were de novo and 11 (32.4%) were inherited with two homozygous and nine heterozygous variants. Fourteen (14.9%) of 94 fetuses had a variant of uncertain significance (VUS). Among 94 cases, six affected pregnancies continued and 88 terminated, and 57 of 88 terminated cases underwent postmortem examinations. With accurate phenotypes demonstrated by prenatal ultrasound and postnatal autopsies, the clinical phenotypes were correlated in 33 (89.2%) of 37 cases with specific genotypes, with the highest matching ratio in skeletal diseases (20/33, 60.6%).ConclusionWES has added value in the genetic diagnosis of abnormal fetuses with normal karyotypes and CMA, particularly in skeletal diseases. Using WES in various anomalous fetuses can broaden the understanding of prenatal phenotypes and genetic variants.
A Novel Model Based on Serum Biomarkers to Predict Primary Non-Response to Infliximab in Crohn’s Disease
Authors: Li Li , Chen Rirong , Zhang Yingfan , Zhou Gaoshi , Chen Baili , Zeng Zhirong , Chen Minhu , Zhang Shenghong 
Published: July 2021

BackgroundInfliximab is effective in inducing and maintaining remission in patients with Crohn’s disease (CD), but primary non-response (PNR) occurs in 10-30% of cases. We investigated whether serum biomarkers are effective in predicting PNR in patients with CD.MethodsFrom January 2016 to April 2020, a total of 260 patients were recruited to this prospective and retrospective cohort study. Serum samples were collected at baseline and week 2 of infliximab treatment. Serum levels of 35 cytokines were assessed in 18 patients from the discovery cohort and were further evaluated in the 60-patient cohort 1. Then, candidate cytokines and other serological biomarkers were used to construct a predictive model by logistic regression in a 182-patient cohort 2. PNR was defined based on the change of CD activity index or clinical symptoms.ResultsAmong the 35 cytokines, matrix metalloproteinase 3(MMP3) and C-C motif ligand 2 (CCL2) were two effective serum biomarkers associated with PNR in both the discovery cohort and cohort 1. In cohort 2, serum level of MMP3, CCL2 and C-reactive protein (CRP) at 2 weeks after infliximab injection were independent predictors of PNR, with odds ratios (95% confidence interval) of 1.108(1.059-1.159), 0.940(0.920-0.965) and 1.102(1.031-1.117), respectively. A PNR classifier combining these three indicators had a large area under the curve [0.896(95% CI:0.895-0.897)] and negative predictive value [0.918(95%CI:0.917-0.919)] to predict PNR to infliximab.ConclusionsMMP3, CCL2, and CRP are promising biomarkers in prediction of PNR to infliximab, and PNR classifier could accurately predict PNR and may be useful in clinical practice for therapy selection.
Fecal Bacteria Implicated in Biofilm Production Are Enriched and Associate to Gastrointestinal Symptoms in Patients With APECED – A Pilot Study
Authors: Hetemäki Iivo , Jian Ching , Laakso Saila , Mäkitie Outi , Pajari Anne-Maria , de Vos Willem M. , Arstila T. Petteri , Salonen Anne 
Published: July 2021

Backgrounds and AimsAPECED is a rare autoimmune disease caused by mutations in the Autoimmune Regulator gene. A significant proportion of patients also have gastrointestinal symptoms, including malabsorption, chronic diarrhea, and obstipation. The pathological background of the gastrointestinal symptoms remains incompletely understood and involves multiple factors, with autoimmunity being the most common underlying cause. Patients with APECED have increased immune responses against gut commensals. Our objective was to evaluate whether the intestinal microbiota composition, predicted functions or fungal abundance differ between Finnish patients with APECED and healthy controls, and whether these associate to the patients’ clinical phenotype and gastrointestinal symptoms.MethodsDNA was isolated from fecal samples from 15 patients with APECED (median age 46.4 years) together with 15 samples from body mass index matched healthy controls. DNA samples were subjected to analysis of the gut microbiota using 16S rRNA gene amplicon sequencing, imputed metagenomics using the PICRUSt2 algorithm, and quantitative PCR for fungi. Extensive correlations of the microbiota with patient characteristics were determined.ResultsAnalysis of gut microbiota indicated that both alpha- and beta-diversity were altered in patients with APECED compared to healthy controls. The fraction of Faecalibacterium was reduced in patients with APECED while that of Atopobium spp. and several gram-negative genera previously implicated in biofilm formation, e.g. Veillonella, Prevotella, Megasphaera and Heamophilus, were increased in parallel to lipopolysaccharide (LPS) synthesis in imputed metagenomics. The differences in gut microbiota were linked to patient characteristics, especially the presence of anti-Saccharomyces cerevisiae antibodies (ASCA) and severity of gastrointestinal symptoms.ConclusionsGut microbiota of patients with APECED is altered and enriched with predominantly gram-negative bacterial taxa that may promote biofilm formation and lead to increased exposure to LPS in the patients. The most pronounced alterations in the microbiota were associated with more severe gastrointestinal symptoms.
Systematic Review of the Effect of a Zero-Markup Policy for Essential Drugs on Healthcare Costs and Utilization in China, 2015–2021
Authors: Liu Wen-Yi , Hsu Chia-Hsien , Liu Ting-Jun , Chen Pei-En , Zheng Boyuan , Chien Ching-Wen , Tung Tao-Hsin 
Published: July 2021

Objective: This systematic review aimed to discuss the effects of a zero-markup policy for essential drugs (ZPED) on healthcare costs and utilization in China in the years 2015–2021.Methods: We searched the PubMed, Embase, Scopus, and CINAHL databases for all associated studies carried out from January 1, 2015, to May 31, 2021, without any limitations regarding the language the studies were written in. To prevent selection bias, gray documents were tackled by other means. The methodological approaches were assessed by applying the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and the Newcastle-Ottawa Scale (NOS) collaboration tool.Results: Forty studies were selected at first and then 15 studies that met the inclusion criterion. Most of the studies showed a considerable decrease in total medical spending and drug spending in both outpatient and inpatient services. After the implementation of ZPED, studies showed that the medical services increased and total hospital income sustained, despite a decrease in drug revenue. Minimal or no government subsidy is required from a financial perspective.Conclusions: Although, the government could implement ZEPD with lower medical cost and drug cost to patients, and sustained income for health facilities, we have limited understanding of whether the increase in medical services was induced by the provider or was a response to unmet needs in the population. Further, studies using rigorous and advanced methods to study health policy, patient behaviors, provider behaviors, and government decisions are warranted.
Exploration of the Relationship Among Key Risk Factors of Acute Kidney Injury for Elderly Patients Considering Covid-19
Authors: Chuang Yen-Ching , Tung Tao-Hsin , Chen Jau-Yuan , Chien Ching-Wen , Shen Kao-Yi 
Published: July 2021

Background: Previous systematic reviews and meta-analyses supported the relationship between frailty and risk of acute kidney injury (AKI) in elderly patients. However, few studies evaluated proactive management to wear down AKI risk in such frail populations.Purpose: To understand how AKI risk factors might influence each other and to identify the source factors for clinical decision aids.Methods: This study uses the decision-making trial and evaluation laboratory (DEMATEL) method to establish influential network-relationship diagrams (INRDs) to form the AKI risk assessment model for the elderly.Results: Based on the DEMATEL approach, the results of INRD identified the six key risk factors: comorbidity, malignancy, diabetes, creatinine, estimated glomerular filtration rate, and nutritional assessment. (The statistical significance confidence is 98.423%, which is higher than 95%; the gap error is 1.577%, which is lower than 5%). After considering COVID-19 as an additional risk factor in comorbidity, the INRD revealed a similar influential relationship among the essential aspects.Conclusion: While evaluating the geriatric population, physicians need to pay attention to patients' comorbidities and nutritional assessment; also, they should note patients' creatinine values and glomerular filtration rate. Physicians could establish a preliminary observation index and then design a series of preventive guidelines to reduce the incidence of AKI risk for the elderly.
Development of a Novel Simple Model to Predict Mortality in Patients With Systemic Lupus Erythematosus Admitted to the Intensive Care Unit
Authors: Guo Jinyan , Huang Zhen , Huang Maoxin , He Yujie , Han Bing , Ma Ning , Yu Zujiang , Liu Shengyun , Ren Zhigang 
Published: July 2021

Background: Patients with systemic lupus erythematosus (SLE) may sometimes require admission to the intensive care unit (ICU), and the outcome is poor. The aim of this study was to explore the clinical features of patients with SLE in the ICU, identify prognostic factors, and develop and evaluate a prognostic model to predict in-ICU mortality of patients with SLE.Patients and Methods: This was a single center retrospective study in a tertiary medical institution in China. A total of 480 SLE patients with 505 ICU admissions from 2010 to 2019 were screened, and 391 patients were enrolled. The clinical feature and outcomes of the patients were analyzed. According to the random number table, patients were divided into two mutually exclusively groups named derivation (n = 293) and validation (n = 98). Prognostic factors were identified by a Cox model with Markov Chain Monte Carlo simulation and evaluated by latent analysis. The risk score was developed based on the derivation group and evaluated using the validation group.Results: Among the 391 patients, 348 (89.0%) patients were females. The median age of patients was 34 years, and the median course of SLE was 6 months. The median APACHE II and SLEDAI were 17 and 10, respectively. The average in-ICU mortality was 53.4% (95% CI, 48.5–58.4%). A total of 186 patients were admitted to the ICU due to infection. Pneumonia (320/391, 81.8%) was the most common clinical manifestation, followed by renal disease (246/391, 62.9%). Nine prognostic factors were identified. The model had C statistic of 0.912 (95% CI, 0.889–0.948) and 0.807 (95% CI 0.703–0.889), with predictive range of 5.2–98.3% and 6.3–94.7% for the derivation and validation groups, respectively. Based on distribution of the risk score, 25.3, 49.5, and 25.2% of patients were stratified into the high, average, and low-risk groups, with corresponding in-ICU mortality of 0.937, 0.593, and 0.118, respectively.Conclusion: Nine prognostic factors including age, white blood cell count, alanine transaminase, uric acid, intracranial infection, shock, intracranial hemorrhage, respiratory failure, and cyclosporin A/tacrolimus usage were identified. A prognostic model was developed and evaluated to predict in-ICU mortality of patients with SLE. These findings may help clinicians to prognostically stratify patients into different risk groups of in-ICU mortality, and provide patients with intensive and targeted management.