bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cisplatin +/− rucaparib after preoperative chemotherapy in patients with triple-negative or BRCA mutated breast cancer
Authors: Kalra Maitri , Tong Yan , Jones David R. , Walsh Tom , Danso Michael A. , Ma Cynthia X. , Silverman Paula , King Mary-Claire , Badve Sunil S. , Perkins Susan M. , Miller Kathy D. 
Published: March 2021

AbstractPatients with triple-negative breast cancer (TNBC) who have residual disease after neoadjuvant therapy have a high risk of recurrence. We tested the impact of DNA-damaging chemotherapy alone or with PARP inhibition in this high-risk population. Patients with TNBC or deleterious BRCA mutation (TNBC/BRCAmut) who had >2 cm of invasive disease in the breast or persistent lymph node (LN) involvement after neoadjuvant therapy were assigned 1:1 to cisplatin alone or with rucaparib. Germline mutations were identified with BROCA analysis. The primary endpoint was 2-year disease-free survival (DFS) with 80% power to detect an HR 0.5. From Feb 2010 to May 2013, 128 patients were enrolled. Median tumor size at surgery was 1.9 cm (0–11.5 cm) with 1 (0–38) involved LN; median Residual Cancer Burden (RCB) score was 2.6. Six patients had known deleterious BRCA1 or BRCA2 mutations at study entry, but BROCA identified deleterious mutations in 22% of patients with available samples. Toxicity was similar in both arms. Despite frequent dose reductions (21% of patients) and delays (43.8% of patients), 73% of patients completed planned cisplatin. Rucaparib exposure was limited with median concentration 275 (82–4694) ng/mL post-infusion on day 3. The addition of rucaparib to cisplatin did not increase 2-year DFS (54.2% cisplatin vs. 64.1% cisplatin + rucaparib; P = 0.29). In the high-risk post preoperative TNBC/BRCAmut setting, the addition of low-dose rucaparib did not improve 2-year DFS or increase the toxicity of cisplatin. Genetic testing was underutilized in this high-risk population.
Fault2SHA Central Apennines database and structuring active fault data for seismic hazard assessment
Authors: Faure Walker Joanna , Boncio Paolo , Pace Bruno , Roberts Gerald , Benedetti Lucilla , Scotti Oona , Visini Francesco , Peruzza Laura 
Published: March 2021

AbstractWe present a database of field data for active faults in the central Apennines, Italy, including trace, fault and main fault locations with activity and location certainties, and slip-rate, slip-vector and surface geometry data. As advances occur in our capability to create more detailed fault-based hazard models, depending on the availability of primary data and observations, it is desirable that such data can be organized in a way that is easily understood and incorporated into present and future models. The database structure presented herein aims to assist this process. We recommend stating what observations have led to different location and activity certainty and presenting slip-rate data with point location coordinates of where the data were collected with the time periods over which they were calculated. Such data reporting allows more complete uncertainty analyses in hazard and risk modelling. The data and maps are available as kmz, kml, and geopackage files with the data presented in spreadsheet files and the map coordinates as txt files. The files are available at:https://doi.org/10.1594/PANGAEA.922582.
The influence of environmental and core temperature on cyclooxygenase and PGE2 in healthy humans
Authors: Esh Christopher J. , Chrismas Bryna C. R. , Mauger Alexis R. , Cherif Anissa , Molphy John , Taylor Lee 
Published: March 2021

AbstractWhether cyclooxygenase (COX)/prostaglandin E2 (PGE2) thermoregulatory pathways, observed in rodents, present in humans? Participants (n = 9) were exposed to three environments; cold (20 °C), thermoneutral (30 °C) and hot (40 °C) for 120 min. Core (Tc)/skin temperature and thermal perception were recorded every 15 min, with COX/PGE2 concentrations determined at baseline, 60 and 120 min. Linear mixed models identified differences between and within subjects/conditions. Random coefficient models determined relationships between Tc and COX/PGE2. Tc [mean (range)] increased in hot [+ 0.8 (0.4–1.2) °C; p < 0.0001; effect size (ES): 2.9], decreased in cold [− 0.5 (− 0.8 to − 0.2) °C; p < 0.0001; ES 2.6] and was unchanged in thermoneutral [+ 0.1 (− 0.2 to 0.4) °C; p = 0.3502]. A relationship between COX2/PGE2 in cold (p = 0.0012) and cold/thermoneutral [collapsed, condition and time (p = 0.0243)] was seen, with higher PGE2 associated with higher Tc. A within condition relationship between Tc/PGE2 was observed in thermoneutral (p = 0.0202) and cold/thermoneutral [collapsed, condition and time (p = 0.0079)] but not cold (p = 0.0631). The data suggests a thermogenic response of the COX/PGE2 pathway insufficient to defend Tc in cold. Further human in vivo research which manipulates COX/PGE2 bioavailability and participant acclimation/acclimatization are warranted to elucidate the influence of COX/PGE2 on Tc.
Response of the chemical structure of soil organic carbon to modes of maize straw return
Authors: Zheng Shuqing , Zhang Jiuming , Chi Fengqin , Zhou Baoku , Wei Dan , Kuang Enjun , Jiang Yu , Mi Gang , Chen Yu ping 
Published: March 2021

AbstractElucidating the chemical structure of soil organic matter (SOM) is important for accurately evaluating the stability and function of SOM. Aboveground vegetation directly affects the quantity and quality of exogenous organic matter input into the soil through plant residues and root exudates, which in turn affects soil microbial species, community structure, and activity, and ultimately impacts the chemical structure of SOM. In this study, a 13C nuclear magnetic resonance technique was used to analyze the chemical structure characteristics of soil organic carbon (SOC) under various rates of straw returning combined with rotary tillage and under full straw mulching. The results showed that full straw returning with rotary tillage and full straw mulching more effectively increased the SOC content than reduced rate of straw returning (1/2 and 1/3 of full straw) with rotary tillage. The contents of alkyl C and alkoxy C in the functional groups of SOC under various straw returning treatments were increased compared with those under the treatment of maize stubble remaining in soil (CK). Furthermore, the contents of aromatic C and carboxyl C were decreased, which were consistent with the chemical shift changes of SOC. Compared with CK treatment, straw returning decreased the content of aromatic C in the functional groups of SOC, but increased the content of alkoxy C, which could be associated with the change in integral areas of absorption peaks of alkyl C and alkoxy C moving toward left and right, respectively. The content of total SOC was significantly positively (P < 0.05) correlated with that of alkoxy C and significantly negatively (P < 0.01) correlated with that of aromatic C. The molecular structure of SOC tends to be simplified due to the decreasing in refractory C and the increasing in easily decomposed C after straw returning to the field.
Addressing indirect frequency coupling via partial generalized coherence
Authors: Young Joseph , Homma Ryota , Aazhang Behnaam 
Published: March 2021

AbstractDistinguishing between direct and indirect frequency coupling is an important aspect of functional connectivity analyses because this distinction can determine if two brain regions are directly connected. Although partial coherence quantifies partial frequency coupling in the linear Gaussian case, we introduce a general framework that can address even the nonlinear and non-Gaussian case. Our technique, partial generalized coherence (PGC), expands prior work by allowing pairwise frequency coupling analyses to be conditioned on other processes, enabling model-free partial frequency coupling results. By taking advantage of recent advances in conditional mutual information estimation, we are able to implement our technique in a way that scales well with dimensionality, making it possible to condition on many processes and produce a partial frequency coupling graph. We analyzed both linear Gaussian and nonlinear simulated networks. We then performed PGC analysis of calcium recordings from mouse olfactory bulb glomeruli under anesthesia and quantified the dominant influence of breathing-related activity on the pairwise relationships between glomeruli for breathing-related frequencies. Overall, we introduce a technique capable of eliminating indirect frequency coupling in a model-free way, empowering future research to correct for potentially misleading frequency interactions in functional connectivity analyses.
Triple negative aggressive phenotype controlled by miR-135b and miR-365: new theranostics candidates
Authors: Bertoli Gloria , Cava Claudia , Corsi Fabio , Piccotti Francesca , Martelli Cristina , Ottobrini Luisa , Vaira Valentina , Castiglioni Isabella 
Published: March 2021

AbstractTriple negative breast cancer (TNBC) accounts for about a fifth of all breast cancers and includes a diverse group of cancers. The heterogeneity of TNBC and the lack of target receptors on the cell surface make it difficult to develop specific therapeutic treatments. These aspects cause the high negative prognosis of patients with this type of tumor. The analysis of the molecular profiles of TNBC samples has allowed a better characterization of this tumor, supporting the search for new reliable diagnostic markers. To this end, we have developed a bioinformatic approach to integrate networks of genes differentially expressed in basal breast cancer compared to healthy tissues, with miRNAs able to regulate their expression. We studied the role of these miRNAs in TNBC subtype cell lines. We therefore identified two miRNAs, namely miR-135b and miR-365, with a central role in regulating the altered functional pathways in basal breast cancer. These two miRNAs are differentially expressed in human TNBC immunohistochemistry-selected tissues, and their modulation has been shown to play a role in the proliferation of tumor control and its migratory and invasive capacity in TNBC subtype cell lines. From the perspective of personalized medicine, we managed to modulate the expression of the two miRNAs in organotypic cultures, suggesting their possible use as diagnostic and therapeutic molecules. miR-135b and miR-365 have a key role in TNBC, controlling proliferation and invasion. Their detection could be helpful in TNBC diagnosis, while their modulation could become a new therapeutic tool for TNBC.
Egoistic punishment outcompetes altruistic punishment in the spatial public goods game
Authors: Li Juan , Liu Yi , Wang Zhen , Xia Haoxiang 
Published: March 2021

AbstractThe evolution of costly punishment is a puzzle due to cooperators’ second-order free-riding. Previous studies have proposed many solutions mainly focused on reducing the punishment cost or punishing second-order free riders directly or indirectly. We attempt to explain this confusion from the perspective of punishment motivation, which is why the punisher is willing to pay the cost. The answer is that the punisher is egoistic. Egoistic punishment aims to protect punishers’ own cooperative benefits shared by the defectors. In such case, egoistic punishers would pay a cost in punishing defectors and retrieve lost payoffs from defectors. Here, we examined the evolution and performance of egoistic punishment and compared it with typical altruistic punishment using classic peer-punishment and pool-punishment modes. Results showed egoistic punishment can evolve and effectively promote cooperation within a large parameter range, whether in a well-mixed or structured population, or through peer-punishment or pool-punishment modes. This result is also robust to different strategy-updating rules. The evolution under the pool-punishment mechanism is more complicated. The influence of parameters is counter-intuitive because of cycle dominance; namely, the cost is the key factor to control the level of cooperation and the fine determines the ratio of the punishers and cooperators. Compared with altruistic punishment, egoistic punishment can promote cooperation in a lower-fine and higher-cost area, especially in the pool punishment mode, and the egoistic punishers have stronger survivability. Egoistic punishers represent the natural fairness in a social system. Results revealed that focusing on individual equity can significantly promote collective cooperation. This study provides another explanation for the evolution of costly punishment.
Clustering of neuropsychological traits of preschoolers
Authors: Treviño Mario , Beltrán-Navarro Beatriz , León Ricardo Medina-Coss y , Matute Esmeralda 
Published: March 2021

AbstractNeuropsychological tests (targeting cognitive, linguistic, motor, and executive abilities) are grouped in neuropsychological domains that are thought to be stable through adulthood. However, this assumption does not always hold true, particularly during young children’s early developmental phase. Here, we explored how the neuropsychological profile of typical Spanish-speaking preschoolers varied and consolidated with age. We recruited 643 monolingual Latin-American children from Mexico, Colombia, and Guatemala, with ages spanning from 30 to 71 months of age, and applied a novel neuropsychological examination which combined a total of 52 tests covering five classical neuropsychological domains: receptive, expressive, attention/memory, processing, and executive functions. These tests’ scores uncovered a correlational structure across neuropsychological functions that could not be explained by chance. Notably, these correlations’ overall strength, but not their interdependence across domains, dramatically increased with age. Moreover, by applying conventional clustering techniques to classify the experimental data, we found a stable representation of two clusters of children with distinctive traits, with cultural factors contributing to this classification scheme. We also found that the tasks were well organized in a network of abilities, where nodes with highest highest interconnectedness were those that required multimodal processing. These results contribute to our understanding of children’s ‘normal’ development and could help identify how failure in particular functions forecasts the emergence of neurodevelopmental disorders. Our analytic methods might become useful to characterize individual differences and improve educational practices and interventions.
Analysis of false-negative rapid diagnostic tests for symptomatic malaria in the Democratic Republic of the Congo
Authors: Parr Jonathan B. , Kieto Eddy , Phanzu Fernandine , Mansiangi Paul , Mwandagalirwa Kashamuka , Mvuama Nono , Landela Ange , Atibu Joseph , Efundu Solange Umesumbu , Olenga Jean W. , Thwai Kyaw Lay , Morgan Camille E. , Denton Madeline , Poffley Alison , Juliano Jonathan J. , Mungala Pomie , Likwela Joris L. , Sompwe Eric M. , Rogier Eric , Tshefu Antoinette K. , N’Siala Adrien , Kalonji Albert 
Published: March 2021

AbstractThe majority of Plasmodium falciparum malaria diagnoses in Africa are made using rapid diagnostic tests (RDTs) that detect histidine-rich protein 2. Increasing reports of false-negative RDT results due to parasites with deletions of the pfhrp2 and/or pfhrp3 genes (pfhrp2/3) raise concern about existing malaria diagnostic strategies. We previously identified pfhrp2-negative parasites among asymptomatic children in the Democratic Republic of the Congo (DRC), but their impact on diagnosis of symptomatic malaria is unknown. We performed a cross-sectional study of false-negative RDTs in symptomatic subjects in 2017. Parasites were characterized by microscopy; RDT; pfhrp2/3 genotyping and species-specific PCR assays; a bead-based immunoassay for Plasmodium antigens; and/or whole-genome sequencing. Among 3627 symptomatic subjects, 427 (11.8%) had RDT-/microscopy + results. Parasites from eight (0.2%) samples were initially classified as putative pfhrp2/3 deletions by PCR, but antigen testing and whole-genome sequencing confirmed the presence of intact genes. 56.8% of subjects had PCR-confirmed malaria. Non-falciparum co-infection with P. falciparum was common (13.2%). Agreement between PCR and HRP2-based RDTs was satisfactory (Cohen’s kappa = 0.66) and superior to microscopy (0.33). Symptomatic malaria due to pfhrp2/3-deleted P. falciparum was not observed. Ongoing HRP2-based RDT use is appropriate for the detection of falciparum malaria in the DRC.
Low soil moisture predisposes field-grown chickpea plants to dry root rot disease: evidence from simulation modeling and correlation analysis
Authors: Sinha Ranjita , Irulappan Vadivelmurugan , Patil Basavanagouda S. , Reddy Puli Chandra Obul , Ramegowda Venkategowda , Mohan-Raju Basavaiah , Rangappa Krishnappa , Singh Harvinder Kumar , Bhartiya Sharad , Senthil-Kumar Muthappa 
Published: March 2021

AbstractRhizoctonia bataticola causes dry root rot (DRR), a devastating disease in chickpea (Cicer arietinum). DRR incidence increases under water deficit stress and high temperature. However, the roles of other edaphic and environmental factors remain unclear. Here, we performed an artificial neural network (ANN)-based prediction of DRR incidence considering DRR incidence data from previous reports and weather factors. ANN-based prediction using the backpropagation algorithm showed that the combination of total rainfall from November to January of the chickpea-growing season and average maximum temperature of the months October and November is crucial in determining DRR occurrence in chickpea fields. The prediction accuracy of DRR incidence was 84.6% with the validation dataset. Field trials at seven different locations in India with combination of low soil moisture and pathogen stress treatments confirmed the impact of low soil moisture on DRR incidence under different agroclimatic zones and helped in determining the correlation of soil factors with DRR incidence. Soil phosphorus, potassium, organic carbon, and clay content were positively correlated with DRR incidence, while soil silt content was negatively correlated. Our results establish the role of edaphic and other weather factors in chickpea DRR disease incidence. Our ANN-based model will allow the location-specific prediction of DRR incidence, enabling efficient decision-making in chickpea cultivation to minimize yield loss.
TMAO: a potential mediator of clopidogrel resistance
Authors: Ma Ruisong , Fu Wenwen , Zhang Jing , Hu Xiaorong , Yang Jun , Jiang Hong 
Published: March 2021

AbstractTrimethylamine-N-oxide (TMAO) can activate platelets and increase thrombosis risk in clinical and experimental models. Meanwhile, the patients with coronary artery disease have higher serum TMAO level. However, it remains unknown whether Clopidogrel Resistance (CR) could be attributed to TMAO. The present study aimed investigate the effects of TMAO on clopidogrel in ischemia and reperfusion (IR) model in rats. Clopidogrel could (1) promote the production of platelets, induce an increase in the platelet-larger cell ratio; (2) prolong the tail bleeding time; (3) reduce platelet aggregation function, induced by ADP, and alleviate myocardial thrombus burden. TMAO could partially offset the effects of clopidogrel and induce CR. Thus, the present study demonstrated that circulating TMAO could reduce the inhibitory effects of clopidogrel on platelet aggregation. TMAO may be a potential mediator of clopidogrel resistance.
Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
Authors: Dey Sujan , Awata Takanori , Mitsushita Jumpei , Zhang Dongdong , Kasai Takuya , Matsuura Norihisa , Katayama Arata 
Published: March 2021

AbstractNitrogen fertiliser is manufactured using the industrial Haber–Bosch process, although it is extremely energy-consuming. One sustainable alternative technology is the electrochemical promotion of biological nitrogen fixation (BNF). This study reports the promotion of BNF activity of anaerobic microbial consortia by humin, a solid-phase humic substance, at any pH, functioning as an extracellular electron mediator, to levels of 5.7–11.8 times under nitrogen-deficient conditions. This was evidenced by increased acetylene reduction activity and total nitrogen content of the consortia. Various humins from different origins promoted anaerobic BNF activity, although the degree of promotion differed. The promotion effected by humin differed from the effects of chemical reducing agents and the effects of supplemental micronutrients and vitamins. The promotion of anaerobic BNF activity by only reduced humin without any other electron donor suggested that humin did not serve as organic carbon source but as extracellular electron mediator, for electron donation to the nitrogen-fixing microorganisms. The next generation sequencing (NGS) of partial 16S rRNA genes showed the predominance of Clostridiales (Firmicutes) in the consortia. These findings suggest the effectiveness of humin as a solid-phase extracellular electron mediator for the promotion of anaerobic BNF activity, potentially to serve for the basis for a sustainable technology.
Clinical factors affecting the rate of exodrift after surgery in patients with basic intermittent exotropia
Authors: Kim Seungheon , Ha Suk-Gyu , Suh Young-Woo , Kim Seung-Hyun 
Published: March 2021

AbstractWe investigated the period of postoperative exodrift during follow-up and clinical factors that affect the rate of exodrift after surgery in the patients with intermittent exotropia (IXT). A retrospective review of medical records of patients with exodrift who underwent bilateral rectus recession for IXT was performed. Exodrift was defined as angle of deviation greater than 10 prism diopters (PD) at distance and near. The median survival period of postoperative exodrift was analyzed using Kaplan Meier survival analysis. The patients were divided into two groups according to the median period of postoperative exodrift (early and late group). The weighted Cox’s proportional hazards regression analysis to investigate the risk factors that affect rate of postoperative exodrift was performed. A total of 108 patients was included. The preoperative angle of deviation at distance and near were 30.3 ± 7.2 PD and 29.5 ± 8.6 PD, respectively. The median survival period of postoperative exodrift was 24 months (range, 6–48 months).The angle of deviation at postoperative day 1 in early and late group were − 3.8 ± 5.5 PD (range, − 16–8 PD) and − 7.7 ± 4.6 PD (range, − 16–4 PD) (p < 0.01). Minus value means esodeviation. In regression analysis, the angle of deviation at postoperative day 1 was the significantly related with rate of exodrift (p < 0.01). The median period of exodrift after surgery was 24 months, angle of deviation at postoperative day 1 could affect the rate of exodrift in patients with IXT.
Capric acid secreted by Saccharomyces boulardii influences the susceptibility of Candida albicans to fluconazole and amphotericin B
Authors: Suchodolski Jakub , Derkacz Daria , Bernat Przemysław , Krasowska Anna 
Published: March 2021

AbstractThe effect of capric acid, secreted by the probiotic yeasts Saccharomyces boulardii, was evaluated on the activities of fluconazole (FLC) and amphotericin B (AMB) against pathogenic Candida albicans fungus. The findings indicated that capric acid may be a promising additive for use in combination with FLC. A FLC-capric acid combination led to reduced efflux activity of multidrug resistance (MDR) transporter Cdr1p by causing it to relocalize from the plasma membrane (PM) to the interior of the cell. The above effect occurred due to inhibitory effect of FLC-capric acid combination of ergosterol biosynthesis. However, capric acid alone stimulated ergosterol production in C. albicans, which in turn generated cross resistance towards AMB and inhibited its action (PM permeabilization and cytoplasm leakage) against C. albicans cells. This concluded that AMB should not be administered among dietary supplements containing capric acid or S. boulardii cells.