bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis
Authors: Wei Xian , Xu Man , Wang Chao , Fang Shengjian , Zhang Yu , Wang Weihua 
Published: April 2021

Abstract
Background
Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRNA regulatory relationship was examined among patients with or without AR.

Methods
Microarray assays were performed and the differential expressions of lncRNAs or mRNA were verified through RT-PCR. The lncRNA functions were annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The potential regulatory relationships between lncRNAs and the co-expressed mRNAs were analyzed using Cytoscape software. The expressions of specific lncRNAs and mRNAs were examined using an in vitro cell model.

Results
A total of 57 lncRNAs and 127 mRNAs were dysregulated in the nasal mucosa tissue of patients with AR, compared to those of patients without AR (fold change > 2.0 and P < 0.05). GO and pathway analysis indicated that the lncRNA–co-expressed mRNAs were enriched in several biological processes and cellular signaling pathways related to AR, such as positive regulation of the integrin biosynthetic process, cell adhesion, and leukocyte transendothelial migration. Some lncRNAs regulated the co-expressed genes in a cis- and/or trans-regulatory manner. Furthermore, allergen exposure significantly increased the expression of lnc-CXCL12-4, CXCL12, and CXCR4 in BEAS-2B cells compared to untreated cells (P < 0.01).

Conclusion
The results of the present study suggest that lncRNAs participate in the biological pathways related to AR. Leukocyte transepithelial migration may be a potential target for lncRNAs to regulate allergic inflammation and CXCL12/CXCR4 axis plays an important role in the inflammatory process of AR.
Using organization theory to position middle-level managers as agents of evidence-based practice implementation
Authors: Birken Sarah A. , Currie Graeme 
Published: April 2021

AbstractMiddle-level managers (MLMs; i.e., healthcare professionals who may fill roles including obtaining and diffusing information, adapting information and the intervention, mediating between strategy and day-to-day activities, and selling intervention implementation) have been identified as having significant influence on evidence-based practice (EBP) implementation. We argue that understanding whether and how MLMs influence EBP implementation is aided by drawing upon organization theory. Organization theories propose strategies for increasing MLMs’ opportunities to facilitate implementation by optimizing their appreciation of constructs which we argue have heretofore been treated separately to the detriment of understanding and facilitating implementation: EBPs, context, and implementation strategies. Specifically, organization theory encourages us to delineate different types of MLMs and consider how generalist and hybrid MLMs make different contributions to EBP implementation. Organization theories also suggest that MLMs’ understanding of context allows them to adapt EBPs to promote implementation and effectiveness; MLMs’ potential vertical linking pin role may be supported by increasing MLMs’ interactions with external environment, helping them to understand strategic pressures and opportunities; and how lateral connections among MLMs have the potential to optimize their contribution to EBP implementation as a collective force. We end with recommendations for practice and future research.
Pectus carinatum as the key to early diagnosis of Morquio A syndrome: a case report
Authors: Yamauchi Kento , Hirano Daishi , Wada Miho , Ida Hiroyuki 
Published: April 2021

Abstract
Background
A 20-month-old Asian boy with normal growth presented with genu valgum, kyphosis, and pectus carinatum, with no neurological symptoms. No other symptoms suggestive of mucopolysaccharidoses, for example joint contracture and peculiar facies, were present.

Case presentation
As part of our differential diagnosis we found elevated urine glycosaminoglycans, which triggered further investigation. Detailed examination showed flattening of the ribs, kyphoscoliosis and ovalization of the thoracolumbar vertebral body, strikingly short metacarpals, and very slight cardiac regurgitation. N-Acetylgalactosamine-6-sulfatase levels in the blood and dermal fibroblasts were very low, thus confirming diagnosis of Morquio A within 2 months of presentation. The patient was placed on elosulfase alfa enzyme replacement therapy and followed for 3 years.

Conclusions
This case exemplifies the importance of considering mucopolysaccharidoses as part of the initial differential diagnosis of pediatric patients with skeletal deformities; urine glycosaminoglycan levels and a blood enzyme mucopolysaccharidoses panel are simple screening tests that could lead to early definitive diagnosis.
A line-of-sight channel model for the 100–450 gigahertz frequency band
Authors: Kokkoniemi Joonas , Lehtomäki Janne , Juntti Markku 
Published: April 2021

AbstractThis paper documents a simple parametric polynomial line-of-sight channel model for 100–450 GHz band. The band comprises two popular beyond fifth generation (B5G) frequency bands, namely, the D band (110–170 GHz) and the low-THz band (around 275–325 GHz). The main focus herein is to derive a simple, compact, and accurate molecular absorption loss model for the 100–450 GHz band. The derived model relies on simple absorption line shape functions that are fitted to the actual response given by complex but exact database approach. The model is also reducible for particular sub-bands within the full range of 100–450 GHz, further simplifying the absorption loss estimate. The proposed model is shown to be very accurate by benchmarking it against the exact response and the similar models given by International Telecommunication Union Radio Communication Sector. The loss is shown to be within ±2 dBs from the exact response for one kilometer link in highly humid environment. Therefore, its accuracy is even much better in the case of usually considered shorter range future B5G wireless systems.
On the qualitative analysis of the fractional boundary value problem describing thermostat control model via ψ-Hilfer fractional operator
Authors: Thaiprayoon Chatthai , Sudsutad Weerawat , Alzabut Jehad , Etemad Sina , Rezapour Shahram 
Published: April 2021

AbstractIn this research study, we are concerned with the existence and stability of solutions of a boundary value problem (BVP) of the fractional thermostat control model withψ-Hilfer fractional operator. We verify the uniqueness criterion via the Banach fixed-point principle and establish the existence by using the Schaefer and Krasnoselskii fixed-point results. Moreover, we apply the arguments related to the nonlinear functional analysis to discuss various types of stability in the format of Ulam. Finally, by several examples we demonstrate applications of the main findings.
Ghost guns: spookier than you think they are
Authors: Wintemute Garen J. 
Published: April 2021

AbstractOff-the-books, untraceable “ghost guns” can now be manufactured at home, easily, and in large numbers; they contribute ever more frequently to firearm violence, including hate violence and domestic terrorism. The Bureau of Alcohol, Tobacco, Firearms and Explosives estimates that in 2019 alone, law enforcement agencies recovered more than 10,000 ghost guns. The manuscript describes the current situation and suggests specific actions that state and federal governments can take to avert disaster.
Inter-individual variation in provisioning rate, prey size and number, and links to total prey biomass delivered to nestlings in the Collared Flycatcher (Ficedula albicollis)
Authors: Cauchard Laure , Macqueen Elise Isabella , Lilley Rhona , Bize Pierre , Doligez Blandine 
Published: April 2021

Abstract
Background
In bird species where offspring growth and survival rely on parents’ food provisioning, parents can maximise their fitness by increasing the quantity and/or the quality of preys delivered to their offspring. Many studies have focused on inter-individual variation in feeding rate, yet this measure may not accurately reflect the total amount of food (i.e. energy) provided by parents if there is large variation in the quantity and quality of preys at each feeding. Here, we explored the relative role of individual (sex, age, body condition), breeding (hatching date, brood size) and environmental (temperature) factors on feeding rate, prey number, size and quality, and their contribution to total prey biomass delivered to the nestlings of 164 Collared Flycatcher (Ficedula albicollis) parents in 98 nests.

Results
Preys delivered to the nest were mainly larvae (53.6%) and flying insects (45.6%). Feeding rate increased with brood size and age, and was higher in males than females. Mean prey number decreased, but mean prey size increased, as the season progressed and parents feeding their brood with primary larvae brought more preys per visit. Relationships between feeding rate, mean prey number and size remained when taking into account the provisioning quality: parents brought either a large number of small prey or a small number of larger items, and the force of the trade-offs between feeding rate and mean prey number and size depended on the quality of the provisioning of the parents. Whatever the percentage of larvae among preys in the provisioning, the variance in total prey biomass was foremost explained by feeding rate (65.1% to 76.6%) compared to mean prey number (16.4% to 26%) and prey size (2.7% to 4%).

Conclusions
Our study shows that variation in feeding rate, prey number, size, but not quality (i.e. percentage of larvae), were influenced by individual factors (sex and age) and breeding decisions (brood size and timing of breeding) and that, whatever the provisioning strategy adopted, feeding rate was the best proxy of the total biomass delivered to the nestlings.
Long non‐coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson’s disease cell model
Authors: Lv Kefeng , Liu Yuhua , Zheng Yanbing , Dai Shaowen , Yin Peifeng , Miao Haifeng 
Published: April 2021

Abstract
Backgrounds
Parkinson’s disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in PD.

Methods
SK-N-SH and SK-N-BE cells were treated with MPP+ to establish the MPP+-stimulated cell model of PD, and MALAT1 expression was determined. Then, the effects of MALAT1 depletion on cell proliferation and apoptosis were determined in the MPP+-stimulated cell model of PD. Besides, the correlations between microRNA-135b-5p (miR-135b-5p) and MALAT1 or glycoprotein nonmetastatic melanoma protein B (GPNMB) in MPP+-stimulated cell model of PD were explored.

Results
MALAT1 was increasingly expressed and downregulation of MALAT1 promoted cell proliferation while inhibited apoptosis in MPP+-stimulated cells. Besides, miR-135b-5p was a target of MALAT1 and directly targeted to GPNMB. Further investigation indicated that suppression of MALAT1 regulated cell proliferation and apoptosis by miR-135b-5p/GPNMB axis.

Conclusion
Our findings reveal that MALAT1/miR-135b-5p/GPNMB axis regulated cell proliferation and apoptosis in MPP+-stimulated cell model of PD, providing a potential biomarker and therapeutic target for PD.
The comparison of STA-MCA bypass and BMT for symptomatic internal carotid artery occlusion disease: a systematic review and meta-analysis of long-term outcome
Authors: Cai Shifei , Fan Hao , Peng Chao , Wu Yuzhang , Yang Xinyu 
Published: April 2021

Abstract
Background
Superficial temporal artery (STA)-middle cerebral artery (MCA) bypass surgery is now being widely used in moyamoya disease, and its therapeutic value in SICAO remains divergent.

Methods
A systematic search was performed in PubMed, EMBASE, and Cochrane Databases in Feb. 2020 and updated in Jun. 2019. We have strict inclusion and exclusion criteria. Cochrane Bias Risk Assessment Tool was used to assess the quality of included RCTs. Review Manager 5.3 was used for analysis results in terms of comparing the STA-MCA bypass and BMT. For dichotomous variable outcomes, risk ratios (RRs) and 95% confidence intervals (95%CIs) were calculated for the assessment.

Results
The total patient cohort consisted of 2419 patients, of whom 1188 (49.1%) patients had been grouped in STA-MCA bypass and 1231 (50.9%) patients had been divided into the BMT group. Mean follow-up of included patients was 29 months. The RR of the seven studies was 1.01, and the 95% confidence interval was .89–1.15, with statistical significance, Z = .13, P = .89, sustaining that STA-MCA bypass was not superior to BMT in symptomatic carotid artery occlusion disease.

Conclusions
STA-MCA bypass and BMT were associated with similar rates of a composite of long-term stroke. And the risk of long-term overall stroke was mildly higher with BMT. At present, each patient should receive more precise treatment, by reasonably assessing the individual differences of each patient to reduce the recurrence rate of stroke.
Serum level of brain-derived neurotrophic factor in patients with relapsing–remitting multiple sclerosis: a potential biomarker for disease activity
Authors: Oraby Mohammed I. , El Masry Hatem A. , Abd El Shafy Sanaa S. , Abdul Galil Esraa M. 
Published: April 2021

Abstract
Background
Brain-derived neurotrophic factor (BDNF) is secreted by immune cells in response to neuroimmune and inflammatory cascades as an act to prevent axonal and neuronal damage after various pathological insults. The serum level of BDNF is altered in a diversity of neurological diseases. The aim of this work was to investigate the serum level of BDNF in patients with relapsing–remitting multiple sclerosis and the relation between BDNF and disease activity and severity.

Methods
A case–control study was conducted on 90 subjects: 60 patients with relapsing–remitting multiple sclerosis (30 in relapse and 30 in remission) on different lines of medical treatment and 30 healthy volunteers as a control. Clinical, functional, and radiological evaluation was done for the patients, and all the patients and controls were subjected to assessment of the serum level of BDNF by sandwich-ELISA technique.

Results
The BDNF level was significantly higher in MS patients in relapse than in patients in remission (P value = 0.006). In the remission group, there was no significant linear correlation between different MS patients’ characteristics and BDNF level, while in the relapse group, a positive linear correlation was found between the number of T2 infratentorial lesions and BDNF level (r = 0.402, P = 0.028). There was no statistically significant difference between the BDNF level in patients administered different drugs for MS in both remission and relapse groups (P value > 0.05).

Conclusion
BDNF was significantly higher in relapsing–remitting multiple sclerosis patients in the relapse phase. Attention should be paid to the link between serum BDNF level as a neuroprotective factor and multiple sclerosis; it can be a biomarker for MS activity in the near future.
Intracranial collateral circulation and its role in neurovascular pathology
Authors: Vasquez Harold E. , Murlimanju B. V. , Shrivastava Adesh , Durango-Espinosa Yeider A. , Joaquim Andrei F. , Garcia-Ballestas Ezequiel , Moscote-Salazar Luis Rafael , Agrawal Amit 
Published: April 2021

Abstract
Background
Collateral circulation is a vascular network which maintains the blood flow after the partial blockage of primary vascular pathways. This acts as potential vascular supplementary system and plays important role in the cerebral ischemia.

Main body
Collateral circulation has implications in the management especially related to cerebral endovascular treatment and thrombolytic therapy. It is considered as subsidiary network of vascular channels, which is highly variable. Insufficient arterial supply is due to the hemodynamic compromise because of thromboembolism. Apart from the collaterals, there is additional existence of a group of vessels known as venous collaterals. Their function is variable and they contribute to the augmentation of venous drainage in venous ischemias. Various pharmacological interventions are used to modulate the collaterals, these can prove to be a complementary alternative to the invasive intracerebral interventions.

Conclusions
The aim of this review article is to highlight the importance of cerebral collateral circulation and to discuss the various available pharmacological alternatives available and their current relevance in the management of various neurovascular pathologies.
Endovascular retrieval of a dislocated pushable coil in the common hepatic artery using a cerebral stent retriever
Authors: Shamseldin Mohammed , Stier Albrecht , Hosten Norbert , Puls Ralf 
Published: April 2021

Abstract
Background
This is case of removing a dislocated pushable coil from the common hepatic artery (CHA) as a possible complication of using pushable coils in the embolization of an upper gastrointestinal bleeding (UGIB) from the gastroduodenal artery (GDA) by using a pRESET stent retriever (Phenox, Bochum, Germany) which is utilized mainly for treatment of endovascular stroke.

Case presentation
An 88-year-old female patient was referred to our hospital to get an emergency embolization of the GDA causing an UGIB with a relevant drop of the hemoglobin level. During the routine embolization of the GDA using pushable coils, a complete dislocation of the last coil into the CHA took place leading to a relevant slowing down of the arterial blood flow to the liver. A decision was thereby made to remove the dislocated coil to avoid further possible complications which was successfully achieved.

Conclusions
Various stent retrievers have been proven to be effective in removing dislocated coils during intracerebral coiling of different pathologies. This case report is to our knowledge the first case report proving the high efficacy and safety of using yet another stent retriever, namely a pRESET stent retriever in removing a fully dislocated coil in the abdominal vessels, namely in this case the CHA.
Single-epoch RTK performance assessment of tightly combined BDS-2 and newly complete BDS-3
Authors: Liu Wanke , Wu Mingkui , Zhang Xiaohong , Wang Wang , Ke Wei , Zhu Zhiqin 
Published: April 2021

AbstractThe BeiDou global navigation satellite system (BDS-3) constellation deployment has been completed on June 23, 2020, with a full constellation comprising 30 satellites. In this study, we present the performance assessment of single-epoch Real-Time Kinematic (RTK) positioning with tightly combined BeiDou regional navigation satellite system (BDS-2) and BDS-3. We first investigate whether code and phase Differential Inter-System Biases (DISBs) exist between the legacy B1I/B3I signals of BDS-3/BDS-2. It is discovered that the DISBs are in fact about zero for the baselines with the same or different receiver types at their endpoints. These results imply that BDS-3 and BDS-2 are fully interoperable and can be regarded as one constellation without additional DISBs when the legacy B1I/B3I signals are used for precise relative positioning. Then we preliminarily evaluate the single-epoch short baseline RTK performance of tightly combined BDS-2 and the newly completed BDS-3. The performance is evaluated through ambiguity resolution success rate, ambiguity dilution of precision, as well as positioning accuracy in kinematic and static modes using the datasets collected in Wuhan. Experimental results demonstrate that the current BDS-3 only solutions can deliver comparable ambiguity resolution performance and much better positioning accuracy with respect to BDS-2 only solutions. Moreover, the RTK performance is much improved with tightly combined BDS-3/BDS-2, particularly in challenging or harsh conditions. The single-frequency single-epoch tightly combined BDS-3/BDS-2 solution could deliver an ambiguity resolution success rate of 96.9% even with an elevation cut-off angle of 40°, indicating that the tightly combined BDS-3/BDS-2 could achieve superior RTK positioning performance in the Asia–Pacific region. Meanwhile, the three-dimensional (East/North/Up) positioning accuracy of BDS-3 only solution (0.52 cm/0.39 cm/2.14 cm) in the kinematic test is significantly better than that of the BDS-2 only solution (0.85 cm/1.02 cm/3.01 cm) due to the better geometry of the current BDS-3 constellation. The tightly combined BDS-3/BDS-2 solution can provide the positioning accuracy of 0.52 cm, 0.22 cm, and 1.80 cm, respectively.
Are Danish vocational schools ready to implement “smoke-free school hours”? A qualitative study informed by the theory of organizational readiness for change
Authors: Hjort Anneke Vang , Schreuders Michael , Rasmussen Kathrine Højlund , Klinker Charlotte Demant 
Published: April 2021

Abstract
Background
The smoking prevalence is high among students enrolled in vocational education and training, which is considered a lower level of education. The school tobacco policy regarding smoke-free school hours stipulates that students and staff are not allowed to smoke during school hours—inside or outside school premises—and it might be an effective intervention for reducing smoking in vocational schools. For school tobacco policies to be effective, they must be appropriately implemented. A primary predictor for successful implementation is organizational readiness for change. This study seeks to identify and understand the barriers to and facilitators for developing organizational readiness to implement smoke-free school hours in Danish vocational schools.

Methods
Semi-structured interviews and focus groups were carried out with managers and teachers (n = 22 participants) from six vocational schools. The interview guides were informed by “A theory of organizational readiness for change” developed by Weiner, which was also used as a framework to analyze the data.

Results
We identified 13 facilitators and barriers. Nine factors acted as facilitators, including the following: believing that health promotion is a school role and duty; believing that society and workplaces are becoming more smoke-free, and believing that smoke-free school hours is a beneficial strategy to achieve fewer educational interruptions. Additional facilitators include establishing clear rules for sanctioning and enforcement, developing a joint understanding about smoke-free school hours, developing skills to deal with student responses to smoke-free school hours, establishing social alternatives to smoking, offering smoking cessation help, and mandating smoke-free school hours by law. Four organizational norms, practices, or discourses acted as barriers: believing that smoke-free school hours violate personal freedom, believing that students have more important problems than smoking, believing that it is difficult to administer the level of enforcement, and believing that the enforcement of smoke-free school hours negatively influences student-teacher relations.

Discussion
Our results suggest that developing organizational readiness before adopting a comprehensive tobacco policy such as smoke-free school hours is important for successful implementation. Further research should investigate how to strengthen the facilitators for and counter the barriers to developing readiness for implementing smoke-free school hours.