bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ropivacaine Inhibits Wound Healing by Suppressing the Proliferation and Migration of Keratinocytes via the PI3K/Akt/mTOR Pathway
Authors: Wu Xiaoyang , Sun Quanyu , He Simeng , Wu Ya , Du Shihan , Gong Lirong , Yu Jianbo , Guo Haifeng 
Published: December 2021

Abstract
Background
After surgery, millions of people suffer from delayed healing or wound dehiscence with subsequent severe complications, even death. Previous studies have reported that ropivacaine exhibits anti-proliferative and anti-migratory activities on numerous cells. Whether ropivacaine is able to influence the proliferation and migration of keratinocytes is still unclear. This study aimed to investigate the effect of ropivacaine and migration on keratinocytes and its underlying molecular mechanism.
Methods
Adult male Sprague-Dawley rats were allocated to establish wound healing models with or without 0.75% ropivacaine treatment and assessed the epidermal thickness by HE staining. HaCaT cells were cultured to evaluate the effect of ropivacaine on wound healing. The cell proliferation, apoptosis status and migration were detected in vitro. Moreover, western blotting was used to examine expression related with PI3K/AKT/mTOR signaling pathways for molecular studies and the changes in inflammatory factors (IL-6, IL-10, TNF-α) were detected by ELISA.
Results
In the present study, we found that ropivacaine delayed wound closure in vivo. In vitro experiments, it was demonstrated that ropivacaine significantly inhibited the proliferation and migration of HaCaT cells via the suppression of PI3K/Akt/mTOR signaling pathway. Activation of PI3K/Akt/mTOR signaling pathway reversed the effects of ropivacaine on the proliferation and migration of HaCaT cells. Furthermore, ropivacaine contributed to the release of pro-inflammatory cytokines (IL-6 and TNF-α) and inhibited the secretion of anti-inflammatory cytokines of keratinocytes (IL-10).
Conclusions
Our research demonstrated that ropivacaine treatment showed a more decreased wound closure rate. Mechanistically, we found that ropivacaine suppressed the proliferation and migration of keratinocytes and altered the expression of cytokines by inhibiting PI3K/AKT/mTOR pathway.
Robust Probabilistic Discriminative Model Prediction Tracker via Improved Model Update Strategy
Authors: Chen Shaolong , Qiu Changzhen , Huang Yurong , Zhang Zhiyong 
Published: December 2021

Abstract
In the visual object tracking, the tracking algorithm based on discriminative model prediction have shown favorable performance in recent years. Probabilistic discriminative model prediction (PrDiMP) is a typical tracker based on discriminative model prediction. The PrDiMP evaluates tracking results through output of the tracker to guide online update of the model. However, the tracker output is not always reliable, especially in the case of fast motion, occlusion or background clutter. Simply using the output of the tracker to guide the model update can easily lead to drift. In this paper, we present a robust model update strategy which can effectively integrate maximum response, multi-peaks and detector cues to guide model update of PrDiMP. Furthermore, we have analyzed the impact of different model update strategies on the performance of PrDiMP. Extensive experiments and comparisons with state-of-the-art trackers on the four benchmarks of VOT2018, VOT2019, NFS and OTB100 have proved the effectiveness and advancement of our algorithm.
Improving the Plasmid Stability by a Hok/Sok System for L-Homoserine Production in Escherichia Coli
Authors: Sun Bing-Yao , Tao Xin-Yi , Sui Hua-Lu , Wang Feng-Qing , Liu Qing-Hai , Gao Bei , Zhao Jian , Ma Yu-Shu , Liu Min , Wei Dong-Zhi 
Published: December 2021

Abstract
Background: The production of bioactive compounds using microbial hosts is considered a safe, cost competitive and scalable approach. However, the efficient engineering of cell factories with well stability, such as for the production of L-aspartate family amino acids and derivatives, remains an outstanding challenge.Results: In the work, the toxin/antitoxin system and genome modification strategy were used to construct a stable Escherichia coli strain for L-homoserine production. The metabolic engineering strategies were focused on the enhancement of precursors for L-homoserine synthesis, reinforcement of the NADPH generation and efflux transporters using CRISPR-Cas9 system at the genome level. To improve the plasmid stability, two strategies were explored, including construction of the aspartate-auxotrophic and hok/sok systems. Constructing the auxotrophic complementation system to maintain plasmid stability was failed herein. The plasmid stability was improved by introducing the hok/sok system, resulting in 6.1 g/L (shake flask) and 44.4 g/L (5 L fermenter) L-homoserine production of the final engineered strain SHL19 without antibiotics addition. Moreover, the hok/sok system was also used to improve the plasmid stability for ectoine production, resulting in 36.7% and 46.5% higher titer of ectoine at shake flask and 5L fermenter without antibiotics addition, respectively. Conclusion: This work provides valuable strategies to improve plasmid stability for producing L-aspartate family amino acids and derivatives and eliminate environmental concerns associated with the application of antibiotics.
Measuring adulterants in street drugs: Implementation of drug residue testing using portable mass spectrometry in a Northeastern U.S. harm reduction program, 2019-2020
Authors: Boulad Mathieu , Matsumoto Atsushi , Santelices Claudia , Stopka Thomas J. 
Published: December 2021

Abstract
Background: Fatal opioid overdose deaths involving illicitly manufactured fentanyl continue to escalate in the U.S. Drug checking services, as a harm reduction intervention for people who use drugs, has gained support as an effective strategy to reduce fatal overdoses. We examined implementation of drug checking services using portable devices in a syringe services program in the Northeastern U.S. Methods: Trained staff collected trace drug specimens from used paraphernalia provided by participants who requested drug checking services. All the specimens were tested using a portable mass spectrometer and sub-samples were tested for the detection of fentanyl using fentanyl testing strips. We assessed characteristics of participants who used drug checking services, self-reported types of trace specimens of substances that participants reported providing for testing, the actual mass spectrometer test results of these specimens, and agreement of the mass spectrometer and fentanyl testing strips results in detection of fentanyl and fentanyl analogues. Results: Of 155 unique participants who provided demographic information, 59% identified as male and 74.1% as White, with a mean age of 37.7 years. Based on analysis of 396 specimens tested with the portable mass spectrometer, the most common single substance detected was fentanyl (37.7%), without a trace of heroin or other adulterants, followed by methamphetamine (18.2%), and cocaine (13.6%). Fentanyl and fentanyl analogues were detected in specimens provided by participants that were reported as heroin (60.8%), cocaine (11.1%), and methamphetamine (6.7%). We found modest agreement of testing results between the mass spectrometer and fentanyl testing strips. Conclusions: Use of drug checking services within syringe services programs is in its initial test stages. Knowledge about the contents of substances purchased, and conversations between syringe services program participants and staff, have the potential to facilitate informed decisions to decrease overdose risks through engagement in harm reduction strategies. Through analysis of newly implemented drug checking services, we noted participant characteristics and dissonance between participants’ reports of the trace drug specimens submitted for testing and the actual drugs and adulterants detected by mass spectrometer results, which has implications for overdose risk, highlighting opportunities for harm reduction responses.
Low dose dexamethasone in combination with Remdesivir does not cause immune dysregulation
Authors: Kannan Kalyan Reddy , Damerla Kshiraja , Mitnala Sasikala , Vemula Venkata Krishna , P Naveen Reddy , Vishnubhotla Ravikanth , Duvvur Nageshwar Reddy 
Published: December 2021

Abstract
BackgroundThe administration of Remdesivir/Dexamethasone combination on T and B cell responses in patients with COVID-19 is sparse. To compare cell mediated immune response in patients treated with only Remdesivir and Remdesivir/dexamethasone combinationStudy DesignProspective, cohort studyMethodsRT-PCR positive SARS-CoV-2 patients (n=49) were enrolled. Patients not requiring O2-supplementation were on remdesivir and those requiring were on remdesivir/dexamethasone. Baseline parameters (complete blood picture, inflammatory markers), T and B cells (flow cytometry: day 5 and 30) and neutralizing antibodies (chemiluminescence: day 30) were estimated. Students “t’’ test was used to evaluate the differences.ResultsOf the 49 patients, 26 (Mean age-44.68±13.45) were treated with remdesivir and 23 (Mean age-48.33±14.76) with remdesivir/dexamethasone combination. While blood counts and immune cells increased, inflammatory markers decreased post treatment in both the groups. A significant increase in WBC (6357±981Vs10700±1363; p=0.01), absolute neutrophil counts (4578±711Vs8256±1323; p=0.01) with decrease in levels of CRP (31±7.1Vs10±4.2; p=0.01), D-Dimer (172.3±8Vs118.1±12; p=0.0005), IL6 (17.6±3.8Vs1.72±0.2; p=0.0001), CD4 cells (2681±86Vs1256±369; p=0.0003), CD8 cells (1749±88 Vs 1256±221; p=0.01) and neutralizing antibodies were seen post treatment in the combination group. Correction of hypoxia and maintenance of oxygen >95% was also noted. ConclusionRemdesivir/dexamethasone combination given to patients requiring oxygen supplementation is safe at 4-6mg/day and showed a marked decrease in inflammation and no immune dysregulation.
Parents’ and teachers’ perceptions of emotional and behavioural problems of school-going adolescents
Authors: Harikrishnan U. , Sailo Grace Lalhlupuii 
Published: December 2021

Abstract
Self-reported studies alone cannot be used to deduce the significance of adolescent problems. Therefore, the current study focuses on parents’ and class teachers’ perspectives of school-going adolescents’ emotional and behavioural problems. The objectives are to apprehend the prevalence of adolescent problems, comparisons on gender, school-area; and its association with socio-demographic details. A cross-sectional questionnaire-based study was conducted among 19 schools from government-private and rural-urban schools across Kollam District, Kerala. Malayalam/English version of the Strength and Difficulties Questionnaire (SDQ) was administered among a sample of 600 parents and 60 class teachers of school-going adolescents. Gender correlation with parents’ report found emotional problems (P<0.01), conduct problems (P<0.01), hyperactivity (P<0.001), peer problems (P<0.001) whereas in teachers’ report found relationship with emotional problems (P<0.05) and hyperactivity (P<0.001). Multivariate test results reveal that there is a statistically significant difference in emotional and behavioural problems of school-going adolescents based on school area (government-urban, government-rural, private-urban and private-rural). Multiple linear regression analysis on parents reports is significantly predicted with gender (P<0.01), urban-rural settings (P<0.001) and socioeconomic status (P<0.01). Teachers’ report has significantly been predicted with urban-rural settings (P<0.01) and socioeconomic status (P<0.001). Parents’ reports revealed a less overall prevalence of emotional and behavioural problems than teachers’ reports. Female adolescents tend to have more emotional problems and males were found to be more hyperactive. A significant difference was found with the school-area settings and significant association with socio-demographic details of adolescents. More attention is needed for the protection of adolescents’ mental health and fills mental health gaps in services.
Within-subjects Effects of Standardized Prosthetic Socket Modifications on Physical Function and Patient-Reported Outcomes
Authors: Anderst William , Fiedler Goeran , Onishi Kentaro , McKernan Gina , Gale Tom , Paulus Paige 
Published: December 2021

Abstract
• Background: Among the challenges of living with lower limb loss is the increased risk of long-term health problems that can be either attributed directly to the amputation surgery and/or prosthetic rehabilitation or indirectly to a disability-induced sedentary lifestyle. These problems are exacerbated by poorly fit prosthetic sockets. There is a knowledge gap regarding how the socket design affects in-socket mechanics, and how in-socket mechanics affect patient-reported comfort and function. The objectives of this study are: 1) to gain a better understanding of how in-socket mechanics of the residual limb in transfemoral amputees are related to patient-reported comfort and function, 2) to identify clinical tests that can streamline the socket design process, and 3) to evaluate the efficacy and cost of a novel, quantitatively informed socket optimization process.• Methods: Users of transfemoral prostheses will be asked to walk on a treadmill wearing their current socket plus 8 different check sockets with designed changes in different structural measurements that are likely to induce changes in residual limb motion, skin strain, and pressure distribution within the socket. Dynamic biplane radiography and pressure sensors will be used to measure in-socket residual limb mechanics. Patient-reported outcomes will also be collected after wearing each socket. The effects of in-socket mechanics on both physical function and patient-reported outcomes (aim 1) will be assessed using a generalized linear model. Partial correlation analysis will be used to examine the association between research grade measurements and readily available clinical measurements (aim 2). In order to compare the new quantitative design method to the Standard of Care, patient reported outcomes and cost will be compared between the two methods, utilizing the Wilcoxon Mann-Whitney non-parametric test (aim 3).• Discussion: Knowledge on how prosthetic socket modifications affect residual bone and skin biomechanics itself can be applied to devise future socket designs, and the methodology can be used to investigate and improve such designs, past and present. Apart from saving time and costs, this may result in better prosthetic socket fit for a large patient population, thus increasing their mobility, participation, and overall health-related quality of life. • Trial registration: clinicaltrials.gov: NCT05041998
Salt-Induced Damage Alleviation in Tanacetum Balsamita L. by Foliar Application of Dobogen Biofertilizer, Glucose and KNO3
Authors: Hassanpouraghdam Mohammad Bagher , Mehrabani Lamia Vojodi , Kheiri Mehdi , Chrysargyris Antonios , Tzortzakis Nikolaos 
Published: December 2021

Abstract
The effects of NaCl salinity stress and foliar application of KNO3, glucose and Dobogen were tested on Tanacetum balsamita. The results showed the significant interaction effects of salinity and foliar sprays on chlorophyll a, K+, Na+, Mg2+, Fe2+, Zn2+, Mn2+ and Si content, K/Na ratio and total phenolic and flavonoid contents. The highest phenolic content was acquired with 100 mM salinity and foliar spray of Dobogen and glucose, 50 mM NaCl × KNO3 application and 50 mM salinity× nonfoliar application. The highest K/Na ratio was observed in control plants and controls × KNO3 and/or Dobogen application. The greatest Si content was recorded with controls × Dobogen and KNO3 applications and no saline × no foliar control plants. Malondialdehyde, flavonoid and proline contents as well as catalase activity were influenced by the independent effects of treatments. Chlorophyll b and superoxide dismutase were affected by salinity. Total soluble solids and Ca2+ were influenced by foliar applications. Malondialdehyde and proline were the highest at 150 mM salinity. Salinity adversely affected the physiological responses of costmary. However, foliar treatments partially ameliorated the salinity effect, and the results would be advisable to the extension section and pioneer farmers.
Variation entry of sediment, organic matter and different forms of phosphorus and nitrogen in flood and normal events in the Anzali wetland
Authors: Ebrahimi Eisa , Asadi Hossein , Joudi Mehrnosh , Rashti Mehran Rezaei , Farhangi Mohammad Bagher , Ashrafzadeh Afshin , Khodadadi Maral 
Published: December 2021

Abstract
Phosphorus (P), nitrogen (N) and organic component are released from the soil into surface bodies by runoff and erosion, causing the pollution and eutrophication of water resources. This study aimed to investigate the seasonal changes in loads of N and P components, and organic matter (OM) in the Pasikhan River in flood and normal conditions. Sampling was performed monthly during normal flow conditions and for five flood events. The concentrations of P and N components were measured in the samples. The OM of the suspended sediments was determined by the combustion method. Under the normal flows, the maximum loads of total P and OM were about 0.553 mg l−1 (STD = 0.11) (November) and 3.41% (STD = 1.17) (November) in the autumn, respectively. The total N concentration of 0.533 mg l−1 (STD = 0.12) was observed in the winter. In the flood events, the TP, TN and OM ranged from 0.48 to 3.5 mg l−1, 0.28 to 0.79 mg l−1 and 1.24 to 4.11%, respectively. The results indicated a high risk of eutrophication in the Pasikhan River. Also, the study revealed that in a severe flood event, some 113.9 tons h−1 of OM can be released from the river watershed. Furthermore, there was a high correlation between the amount of P and OM losses with the concentration of suspended sediments in different flood events. Finally, it is concluded that if the floods are not controlled, they not only cause a rapid loss of soil nutrients and OM but also lead to severe eutrophication in the Anzali wetland.
Ks. dziekan Franciszek Pogrzeba (1873-1941) - duszpasterz, społecznik i górnośląski działacz plebiscytowy 1921 r.
Authors: Patelski Mariusz 
Published: December 2021

Prezentowany artykuł jest przyczynkiem do biografii duchownego katolickiego, proboszcza parafii w Łubnianach na Górnym Śląsku – ks. Franciszka Pogrzeby. W 1907 r. ks. Pogrzeba, jako jeden z czternastu kapłanów, podpisał tzw. „Odezwę księży”, w myśl której zerwali oni współpracę z katolicką Niemiecką Partią Centrum oraz wsparli posłów „Koła Polskiego” w parlamencie niemieckim. Po I wojnie światowej ks. Pogrzeba działał na rzecz przyłączenia Górnego Śląska do Polski. Po podziale tego regonu, zagrożony przez niemieckich nacjonalistów, musiał uciekać do Polski. Resztę życia spędził jako proboszcz parafii w Przyszowicach, położonych w należącej do Polski części Górnego Śląska.
Evaluation of Repeated Web-Based Screening for Predicting Postpartum Depression: Prospective Cohort Study
Authors: Haßdenteufel Kathrin , Lingenfelder Katrin , Schwarze Cornelia E , Feisst Manuel , Brusniak Katharina , Matthies Lina Maria , Goetz Maren , Wallwiener Markus , Wallwiener Stephanie 
Published: December 2021

Background
Postpartum depression (PPD) is a severe mental disorder that often results in poor maternal-infant attachment and negatively impacts infant development. Universal screening has recently been recommended to identify women at risk, but the optimal screening time during pregnancy has not been defined so far. Thus, web-based technologies with widespread use among women of childbearing age create new opportunities to detect pregnancies with a high risk for adverse mental health outcomes at an early stage.

Objective
The aim of this study was to stratify the risk for PPD and to determine the optimal screening time during pregnancy by using a web-based screening tool collecting electronic patient-reported outcomes (ePROs) as the basis for a screening algorithm.

Methods
In total, 214 women were repeatedly tested for depressive symptoms 5 times during and 3 times after pregnancy by using the Edinburgh Postnatal Depression Scale (EPDS), accessible on a web-based pregnancy platform, developed by the authors of this study. For each prenatal assessment, the area under the curve (AUC), sensitivity, specificity, and predictive values for PPD were calculated. Multivariate logistic regression analyses were applied to identify further potential predictors, such as age, education, parity, relationship quality, and anxiety, to increase predictive accuracy.

Results
Digitally collected data from 214 pregnant women were analyzed. The predictive accuracy of depressive symptoms 3 and 6 months postpartum was reasonable to good regarding the screening in the second (AUC=0.85) and third (AUC=0.75) trimester. The multivariate logistic regression analyses resulted in an excellent AUC of 0.93 at 3 months and a good AUC of 0.87 at 6 months postpartum.

Conclusions
The best predictive accuracy for PPD has been shown for screening between the 24th and the 28th gestational week (GW) and seems to be beneficial for identifying women at risk. In combination with the aforementioned predictive factors, the discriminatory power improved, particularly at 3 months postpartum. Screening for depression during pregnancy, combined with the women’s personal risk profile, can be used as a starting point for developing a digital screening algorithm. Thereby, web-based assessment tools constitute feasible, efficient, and cost-effective approaches. Thus, they seem to be beneficial in detecting high-risk pregnancies in order to improve maternal and infant birth outcomes in the long term.
The Effect of Digital Health Interventions on Parents’ Mental Health Literacy and Help Seeking for Their Child’s Mental Health Problem: Systematic Review
Authors: Peyton Daniel , Goods Marquelle , Hiscock Harriet 
Published: December 2021

Background
Many children with mental health problems do not receive professional help. Despite the frequent use of digital health interventions (DHIs) such as websites or web-based service navigation platforms, their effects on parents’ mental health literacy, help seeking, or uptake of professional services are unclear.

Objective
This study aims to provide a systematic review and narrative synthesis to describe whether DHIs improve the aforementioned parental outcomes.

Methods
Databases, including CINAHL, Embase, MEDLINE OVID, PsycINFO, and PubMed (2000-2020), were accessed. Studies were included if they evaluated quantitative changes in mental health literacy, help seeking, or the uptake of services by parents of children with mental health problems. Theoretical frameworks, sample sizes, participant demographics, recruitment, interventions, DHI use, results, and health economic measures were used for data extraction.

Results
Of the 11,379 search results, 5 (0.04%) studies met the inclusion criteria. One randomized controlled trial found the reduced uptake of services after using a DHI coupled with a telephone coach for a child’s behavioral problem. Of 3 studies, 2 (66.7%) found statistically significant improvement in mental health literacy for attention-deficit/hyperactivity disorder but had no control group. One study found nonsignificant improvement in mental health literacy and help-seeking attitudes toward anxiety and depression compared with those in active controls. All studies were rated as having a high or serious risk of bias. Search results were affected because of a single reviewer screening articles, overall low-quality studies, and a lack of consistent nomenclature.

Conclusions
There is no high-quality evidence that DHIs can improve parents’ mental health literacy, help seeking, or uptake of services. More research is needed to evaluate DHIs by using rigorous study designs and consistent measures.

Trial Registration
PROSPERO International Prospective Register of Systematic Reviews CRD42020130074; https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020130074