bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sediment resuspension and transport from a glider integrated Laser In Situ Scattering and Transmissometry (LISST) particle analyzer
Authors: Miles Travis , Slade Wayne , Glenn Scott 
Published: May 2021

AbstractSuspended particle size and concentration are critical parameters necessary to understand water quality, sediment dynamics, carbon flux, and ecosystem dynamics among other ocean processes. In this study we detail the integration of a Sequoia Scientific, Inc., Laser In situ Scattering and Transmissometry (LISST) sensor into a Teledyne Webb Research Slocum autonomous underwater glider. These sensors are capable of measuring particle size, concentration, and beam attenuation by particles in size ranges from 1.00 to 500 μm at a resolution of 1 Hz. The combination of these two technologies provides the unique opportunity to measure particle characteristics persistently at specific locations, or survey regional domains from a single profiling sensor. In this study we present the sensor integration framework, detail quality assurance and control (QAQC) procedures, as well as provide a case study of storm driven sediment resuspension and transport. Specifically, Rutgers glider RU28 was deployed with an integrated LISST-Glider for 18 days in September of 2017. During this time period it sampled the nearshore environment off of coastal New Jersey, capturing full water column sediment resuspension during a coastal storm event. A novel method for in situ background corrections is demonstrated and used to mitigate long-term bio-fouling of the sensor windows. Additionally, we present a method for removing Schlieren contaminated time periods utilizing coincident conductivity temperature and depth, fluorometer, and optical backscatter data. The combination of LISST sensors and autonomous platforms has the potential to revolutionize our ability to capture suspended particle characteristics throughout the world’s oceans.
Who, the people? Rethinking constituent power as praxis
Authors: van Asseldonk Maxim 
Published: May 2021

Modern thinking about democracy is largely governed by the concept of constituent power. Some versions of the concept of constituent power, however, remain haunted by the spectre of totalitarianism. In this article, I outline an alternative view of the identity of the people whose constituent power generates democratic authority. Broadly speaking, constituent power signifies the idea that all political authority, including that of the constitution, must find its source in some idea of ‘the people’, whose authority is never exhausted by constituted power. The deficiency I seek to address is that of asking who the people is to whom any claim of authority refers, while avoiding the pitfalls of totalitarianism. I show the most famous totalitarian view of constituent power – advanced by Carl Schmitt – to be not only politically unsavoury but also ontologically unjustified. To outline my alternative view, I draw on Jacques Derrida’s concept of just decisions to argue that the undecidable inaugurates collective responsibility by demanding a response. This suggests a view of ‘the people’ as a doing rather than a being. I conclude by showing how this avoids totalitarian views of popular sovereignty by demonstrating its congruency with Claude Lefort’s democratic theory as opposed to totalitarianism.
Social impact scaling strategies in social enterprises: A systematic review and research agenda
Authors: Islam Syrus M 
Published: May 2021

Social enterprises have attracted increased attention from both researchers and practitioners around the world. In the social enterprise context, scaling social impact is considered the main currency or key performance metric. Two overarching social impact scaling strategies are organizational growth strategy and ecosystem growth strategy. However, to date, little cumulative knowledge exists on these two social impact scaling strategies. To address this issue, this article conducts a systematic review of 111 peer-reviewed articles. It identifies and discusses key insights into organizational growth strategy and ecosystem growth strategy as a means to scale social impact in social enterprises. Based on these findings, the current article also develops a framework to facilitate a comprehensive understanding of social impact scaling strategies in social enterprises. Finally, the review identifies gaps in the existing literature and discusses a comprehensive agenda for future research. JEL Classification: L26, L31, O35, M13
Exploring Mechanisms of Mental Illness Stigma Reduction in Asian Canadian Men
Authors: Fung Kenneth Po-Lun , Liu Jenny Jing-Wen , Wong Josephine Pui-Hing 
Published: May 2021

Objective: Although there is evidence of effective stigma reduction by various psychological and educational interventions, the mechanisms of change remain unclear. In this article, we examine hypothesized processes that might have mediated reductions in stigma observed among Asian men who had received in mental health promotion interventions in Greater Toronto Area, Canada. Method: Our sample consisted of 495 Asian men, who received either acceptance and commitment therapy (ACT; n = 133), contact-based empowerment education (CEE; n = 149), combination of ACT and CEE ( n = 152), or psychoeducation ( n = 61). Group differences on intervention outcomes, including stigmatizing attitudes (Community Attitudes toward the Mentally Ill), internalized stigma (Internalized Stigma of Mental Illness), valued living (Valued Living Questionnaire), and attitudes to engage in social change (Social Justice Scale) were hypothesized to be due to the impact of the different interventions and mediated by changes in specific underlying psychological processes. These process-related changes were modelled using measures of mindfulness (Freiburg Mindfulness Inventory), psychological flexibility (Acceptance and Action Questionnaire version II), and empowerment (Empowerment Scale [ES]). Their pre- and post changes were analyzed with repeated measures analysis of variance, and mediational analyses were performed. Results: Findings from mediational analyses suggest that empowerment (ES) mediated a significant portion of the effects observed in reduction in stigmatizing attitudes and internalized stigma across intervention groups ( t = 3.67 to 3.78 for CEE groups, and t = 4.32 to 4.56 for ACT groups). For the ACT groups, reduction in internalized stigma might also have been partly mediated by psychological flexibility, an intervention-specific psychological process. Conclusions: Results from the current study suggest that different stigma reduction interventions may be mediated by increased empowerment as a common mechanism of change, while intervention-specific mechanism of change, improved psychological flexibility through ACT, may also contribute to improvement in internalized stigma.
An ultrasound method for characterization of viscoelastic properties in frequency domain at small deformations
Authors: Sakhnevych A , Genovese A , Maiorano A , Timpone F , Farroni F 
Published: May 2021

Background The ultrasound technique, usually based on the transmission mode, is capable of providing the viscoelastic properties of polymers. Further techniques involving pulse-echo methods were also described in literature, but they still exhibit inaccuracies in the evaluation of the acoustic properties. Objective The manuscript focuses on an innovative approach for the characterization of the viscoelastic behavior of polymers employing the ultrasound methodology. The proposed procedure is based on the pulse-echo method in order to overcome possible inaccuracies in acoustic properties evaluation and in issues related to transmitter mode applications. Methods Starting from the pulse-echo method adopted for the acquisition, a novel formulation for data processing has been developed and described, allowing to determine the wave attenuation coefficient, in comparison to the commonly employed procedures involving ultrasound in polymers characterization, based on transmitter mode inspections. To carry out the study, a specifically designed ultrasound bench has been set up and three different polymers have been tested in the temperature range of interest. Results According to the proposed methodology, the loss factor towards the temperature is determined starting from the data acquired considering the identified attenuation coefficient and the measured sound velocities. The trustworthiness of the novel procedure has been proved comparing the obtained viscoelastic loss factor quantities to the reference master curves obtained by the standard Dynamic Mechanical Analysis characterizations carried out on the same polymer specimens. Conclusions A novel methodology involving ultrasound technology aiming to evaluate the viscoelasticity of the polymers using non-destructive approach has been developed. The results obtained are agreement with the standard viscoelastic master curves determined through the DMA.
‘The Jigsaw Culture of Care’: A qualitative analysis of Montessori-Based programming for dementia care in the United Kingdom
Authors: Raghuraman Shruti , Tischler Victoria 
Published: May 2021

Aims Montessori-Based Programming (MBP) in dementia care refers to a growing body of research and practice that has developed Montessori methods to facilitate self-paced learning, independence and engagement for people living with dementia. A number of research gaps have been identified in the existing literature such as a lack of cross-cultural studies and well-powered, robustly designed outcome studies. The current study investigated the use of MBP with a focus on provision in the United Kingdom. It aimed to identify MBP implementation approaches, challenges and barriers, and research gaps. Design and Methods A qualitative design was implemented to analyse data from in-depth, semi-structured interviews with key stakeholders ( N = 8) with experience of MBP in the UK. Participants included care home management and staff, MBP trainers and independent dementia experts with a background in Montessori methods. Thematic analysis identified 4 main themes and 12 sub-themes. The study took place between April 2019 and October 2019. Findings A framework describing knowledge and understanding of MBP in the UK, implementation considerations, challenges and barriers, evidence of outcomes and research gaps was developed to provide guidance for researchers and practitioners. Implementation considerations included using a whole-home approach and changing the culture of care through management support. Barriers to implementation included conservative attitudes to care, perceived lack of time and resources, health and safety issues, and issues of sustainability. Conclusion The benefits of MBP in dementia care are promising but require further empirical investigation. There is a need to design, execute and publish evidence to secure the support of key stakeholders in dementia care research, policy and commissioning in the UK.
Taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism
Authors: Méndez-Salazar Eder Orlando , Vázquez-Mellado Janitzia , Casimiro-Soriguer Carlos S. , Dopazo Joaquin , Çubuk Cankut , Zamudio-Cuevas Yessica , Francisco-Balderas Adriana , Martínez-Flores Karina , Fernández-Torres Javier , Lozada-Pérez Carlos , Pineda Carlos , Sánchez-González Austreberto , Silveira Luis H. , Burguete-García Ana I. , Orbe-Orihuela Citlalli , Lagunas-Martínez Alfredo , Vazquez-Gomez Alonso , López-Reyes Alberto , Palacios-González Berenice , Martínez-Nava Gabriela Angélica 
Published: May 2021

Abstract
Objective
To evaluate the taxonomic composition of the gut microbiome in gout patients with and without tophi formation, and predict bacterial functions that might have an impact on urate metabolism.

Methods
Hypervariable V3–V4 regions of the bacterial 16S rRNA gene from fecal samples of gout patients with and without tophi (n = 33 and n = 25, respectively) were sequenced and compared to fecal samples from 53 healthy controls. We explored predictive functional profiles using bioinformatics in order to identify differences in taxonomy and metabolic pathways.

Results
We identified a microbiome characterized by the lowest richness and a higher abundance of Phascolarctobacterium, Bacteroides, Akkermansia, and Ruminococcus_gnavus_group genera in patients with gout without tophi when compared to controls. The Proteobacteria phylum and the Escherichia-Shigella genus were more abundant in patients with tophaceous gout than in controls. Fold change analysis detected nine genera enriched in healthy controls compared to gout groups (Bifidobacterium, Butyricicoccus, Oscillobacter, Ruminococcaceae_UCG_010, Lachnospiraceae_ND2007_group, Haemophilus, Ruminococcus_1, Clostridium_sensu_stricto_1, and Ruminococcaceae_UGC_013). We found that the core microbiota of both gout groups shared Bacteroides caccae, Bacteroides stercoris ATCC 43183, and Bacteroides coprocola DSM 17136. These bacteria might perform functions linked to one-carbon metabolism, nucleotide binding, amino acid biosynthesis, and purine biosynthesis. Finally, we observed differences in key bacterial enzymes involved in urate synthesis, degradation, and elimination.

Conclusion
Our findings revealed that taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism.
InterMiG: international differences in the therapeutic approach to migraine patients in specialized headache centers
Authors: Gago-Veiga AB , Huhn J-I , Latysheva N , Vieira Campos A , Torres-Ferrus M , Alpuente Ruiz A , Sacco S , Frattale I , Ornello R , Ruscheweyh R , Marques IB , Gryglas-Dworak A , Stark C , Gallardo VJ , Pozo-Rosich P 
Published: May 2021

AbstractBackgroundThere is currently a wide therapeutic arsenal for migraine patients, without a single first-line preventive drug and we choose the different available alternatives taking into account comorbidities, national guidelines, previous treatments and personal experiences.Our objective was to evaluate the differences in the use of migraine treatments between neurologists from different countries.MethodsThis is a multi-centre observational study carried out by neurologists from specialized headache units in seven countries, retrospective with consecutive inclusion of all patients presenting with a migraine diagnosis, over a period of three months.ResultsA total of 734 patients were recruited but only 600 were considered in the analysis in order to homogenize the patient cohorts from countries: 200 Spain (ES), 100 Italy (IT), 85 Russia (RUS), 80 Germany (DE), 60 Portugal (PT), 45 Poland (PL) and 30 Australia (AU). 85.4 % of patients were women with a mean age of 42.6 ± 11.8 years. Considering previous and current preventive treatment, the order of use was: antidepressants (69.3 %), antiepileptic drugs (54.7 %), beta-blockers and antihypertensive drugs (49.7 %), OnabotulinumtoxinA (44.0 %) and others (36.2 %).Statistically significant differences were found between all pharmacological classes: antidepressants were commonly used in all countries, with the exception of Poland (AU: 76.7 %, IT: 71.0 %, DE: 60.0 %, PL: 31.1 %, PT: 71.7 %, RUS: 70.6 %, ES: 78.5 %;p < 0.0001); antiepileptic drugs were more frequently prescribed in Portugal, Australia and Spain (AU: 73.3 %, IT: 40.0 %, DE: 37.5 %, PL: 48.9 %, PT: 85.0 %, RUS: 29.4 % and ES: 69.0 %;p < 0.0001); beta-blockers and antihypertensive drugs were frequently used in all countries except Italy (AU: 60.0 %, IT: 14.0 %, DE: 53.8 %, PL: 48.9 %, PT: 68.3 %, RUS: 49.4 % and ES: 59.0 %;p < 0.0001); BTX-A were predominately used in Spain, Italy and Australia (AU:56.7 %, IT:58.0 %, DE:20.0 %, PL: 42.2 %, PT: 26.7 %, RUS: 24.7 % and ES: 58.5 %;p < 0.0001) and others were most frequently used in Poland (AU: 0.0 %, IT: 19.0 %, DE: 42.5 %, PL: 95.6 %, PT: 31.7 %, RUS: 3.5 % and ES: 49.5 %;p < 0.0001). If only patients without comorbidities are considered (200/600), statistically differences between countries persist in all preventive treatments.ConclusionsThere is heterogeneity in the choice of preventive treatment between different countries. Prospective comparative studies of the different oral and subcutaneous alternatives would help to create a global therapeutic algorithm that would guarantee the best option for our patients.
Moonlighting protein prediction using physico-chemical and evolutional properties via machine learning methods
Authors: Shirafkan Farshid , Gharaghani Sajjad , Rahimian Karim , Sajedi Reza Hasan , Zahiri Javad 
Published: May 2021

AbstractBackgroundMoonlighting proteins (MPs) are a subclass of multifunctional proteins in which more than one independent or usually distinct function occurs in a single polypeptide chain. Identification of unknown cellular processes, understanding novel protein mechanisms, improving the prediction of protein functions, and gaining information about protein evolution are the main reasons to study MPs. They also play an important role in disease pathways and drug-target discovery. Since detecting MPs experimentally is quite a challenge, most of them are detected randomly. Therefore, introducing an appropriate computational approach to predict MPs seems reasonable.ResultsIn this study, we introduced a competent model for detecting moonlighting and non-MPs through extracted features from protein sequences. We attempted to set up a well-judged scheme for detecting outlier proteins. Consequently, 37 distinct feature vectors were utilized to study each protein’s impact on detecting MPs. Furthermore, 8 different classification methods were assessed to find the best performance. To detect outliers, each one of the classifications was executed 100 times by tenfold cross-validation on feature vectors; proteins which misclassified 90 times or more were grouped. This process was applied to every single feature vector and eventually the intersection of these groups was determined as the outlier proteins. The results of tenfold cross-validation on a dataset of 351 samples (containing 215 moonlighting and 136 non-moonlighting proteins) reveal that the SVM method on all feature vectors has the highest performance among all methods in this study and other available methods. Besides, the study of outliers showed that 57 of 351 proteins in the dataset could be an appropriate candidate for the outlier. Among the outlier proteins, there were non-MPs (such as P69797) that have been misclassified in 8 different classification methods with 16 different feature vectors. Because these proteins have been obtained by computational methods, the results of this study could reduce the likelihood of hypothesizing whether these proteins are non-moonlighting at all.ConclusionsMPs are difficult to be identified through experimentation. Using distinct feature vectors, our method enabled identification of novel moonlighting proteins. The study also pinpointed that a number of non-MPs are likely to be moonlighting.
Re-examination of two diatom reference genomes using long-read sequencing
Authors: Filloramo Gina V. , Curtis Bruce A. , Blanche Emma , Archibald John M. 
Published: May 2021

Abstract
Background
The marine diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum are valuable model organisms for exploring the evolution, diversity and ecology of this important algal group. Their reference genomes, published in 2004 and 2008, respectively, were the product of traditional Sanger sequencing. In the case of T. pseudonana, optical restriction site mapping was employed to further clarify and contextualize chromosome-level scaffolds. While both genomes are considered highly accurate and reasonably contiguous, they still contain many unresolved regions and unordered/unlinked scaffolds.

Results
We have used Oxford Nanopore Technologies long-read sequencing to update and validate the quality and contiguity of the T. pseudonana and P. tricornutum genomes. Fine-scale assessment of our long-read derived genome assemblies allowed us to resolve previously uncertain genomic regions, further characterize complex structural variation, and re-evaluate the repetitive DNA content of both genomes. We also identified 1862 previously undescribed genes in T. pseudonana. In P. tricornutum, we used transposable element detection software to identify 33 novel copia-type LTR-RT insertions, indicating ongoing activity and rapid expansion of this superfamily as the organism continues to be maintained in culture. Finally, Bionano optical mapping of P. tricornutum chromosomes was combined with long-read sequence data to explore the potential of long-read sequencing and optical mapping for resolving haplotypes.

Conclusion
Despite its potential to yield highly contiguous scaffolds, long-read sequencing is not a panacea. Even for relatively small nuclear genomes such as those investigated herein, repetitive DNA sequences cause problems for current genome assembly algorithms. Determining whether a long-read derived genomic assembly is ‘better’ than one produced using traditional sequence data is not straightforward. Our revised reference genomes for P. tricornutum and T. pseudonana nevertheless provide additional insight into the structure and evolution of both genomes, thereby providing a more robust foundation for future diatom research.
Brain natriuretic peptide and cardiac troponin I for prediction of the prognosis in cancer patients with sepsis
Authors: Yang Yong , Leng Jiahua , Tian Xiuyun , Wang Hongzhi , Hao Chunyi 
Published: May 2021

Abstract
Background
This article aimed to study the value of brain natriuretic peptide (BNP) and cardiac troponin I(cTnI) for predicting the prognosis in cancer patients with sepsis.

Methods
A cohort of 233 cancer patients with sepsis admitted to our ICU from January 2017 to October 2020 was included in this retrospective study. The data of BNP and cTnI on the first day (d1) and the third day(d3) after entering ICU, blood lactate (Lac), procalcitonin (PCT), Leucocyte and Sequential Organ failure assessment (SOFA) scores within 24 hr of entering ICU, the incidence of septic shock, acute kidney injury(AKI), acute respiratory failure (ARF) or sepsis-induced myocardial dysfunction(SIMD) in ICU, fluid balance in 24 hr and 72 hr after entering ICU, time of mechanical ventilation(MV), length of stay, emergency surgery were collected. According to 28-day mortality, patients were divided into survival group (190 cases) and death group (43 cases). All the above variables were compared.

Results
BNP was an independent predictor for the mortality in these patients (P < 0.05).While cTnI was not. BNP on d3 in 681.5 pg/ml predicted the mortality with a sensitivity of 91.5 % and a specificity of 88.7 %. All patients were divided into the new two groups following the cutoff value of BNP on d3(681.5pg/ml), and the survival curve showed a significant difference with Kaplan-Meier analysis (P < 0.05). BNP had statistical differences between four groups based on the comorbidities(septic shock, AKI, ARF or SIMD), but cTnI was not.

Conclusions
BNP was a great predictor for the prognosis of cancer patients with sepsis, while cTnI was not.