Inonotus obliquus Aqueous Extract Suppresses Carbon Tetrachloride-induced Hepatic Injury through Modulation of Antioxidant Enzyme System and Anti-inflammatory Mechanism
Authors: Ishfaq Pir Mohammad , Mishra Anjali , Mishra Shivani , Ahmad Zaved , Gayen Shovanlal , Jain Subodh Kumar , Tripathi Swati , Mishra Siddhartha Kumar 
Published: December 2021

Background:
Chaga mushroom (Inonotus obliquus) is an edible macrofungus used in
traditional and folk medicine for the treatment of various gastrointestinal disorders. It has shown
potent anti-inflammatory, antioxidant and anticancer effects in several experimental studies, including
anti-inflammatory and anticancer effects in colorectal cancer and intestinal inflammation.
Whole extract or purified compound ergosterol peroxide from chaga mushroom showed anti-inflammatory
mechanism via suppression of NF-κB/iNOS-COX-2 and growth inhibitory mechanism
via regulation of apoptosis activation and β-catenin suppression. The diverse inflammatory and carcinogenic
agents, like carbon tetrachloride (CCl4), a potent hepatotoxic chemical, cause liver damage
by inducing lipid peroxidation and other oxidative damages.

Aims:
The study aimed to analyze the biochemical, cellular and molecular mechanisms of CCl4 induced
chronic liver inflammation and carcinoma, and to analyze the effect of the extract of chaga
mushroom on liver inflammation and cancer by virtue of anti-inflammatory mechanisms.

Method:
Physiological, histological and immunohistochemical analyses of the physiological functions
and cellular functions were performed. Biochemical assays were conducted for assessing enzymatic
changes in tissues. Molecular simulation and docking studies were performed for the evaluation
of the molecular interaction.

Results:
CCl4-exposed mice exhibited a significant decrease in body weight followed by altered histopathological
signatures in the liver. Supplementation of IOAE showed that treatment restored the
normal structure of the tissues with large round nuclei in most of the cells. CCl4 caused a steep elevation
in the levels of SGOT and SGPT to 2.32- and 1.8-fold as compared to control. The LDH level
increased to 447 IU/L in CCl4 treated mice as compared to control (236 IU/L). Analysis of the
oxidant enzyme pathway showed that CCl4 reduced the GSH level to 16.5 μM as compared to control
(52 μM), and induced the catalase enzyme activity up to 259 U/mL as compared to control
(124 U/L). These physiological and biochemical alterations were restored towards normal levels by
IOAE administration. Immunohistochemical staining for caspase-3 and p53 showed that CCl4
notably increased their expressions, which were subsequently suppressed by administration of
IOAE. The molecular simulation and docking studies using ergosterol peroxide from chaga mushroom
with iNOS, COX-2 and TNF-α showed binding energy of -10.5, -8.9 and -9.1 Kcal/mol, respectively.
These proteins interacting with ergosterol peroxide exerted an inhibitory effect on these
critical proinflammatory signaling proteins.

Conclusions:
The results point out that IOAE is able to prevent damage of hepatic cells caused by
CCl4 in mouse models through anti-inflammatory and growth inhibitory mechanisms, which can be
utilized for natural prevention of liver toxicity.