bluuebunny/crossref_metadata_2025_split huggingface dataset:
Publication Trends on Halal Tourism: A Bibliometric Review
Authors: Abdullah Khairul Hafezad 
Published: July 2021

Halal tourism has grown in popularity due to the rise of the Muslim population; howbeit it is still a relatively new field. This study aimed to evaluate halal tourism’s publication trends, research areas, prolific sources, most cited documents, co-authorship concerning authors, institutions and countries, and keywords co-occurrence. Using the Scopus database, 118 halal tourism publications were disseminated between 2010 and 2020. This study found an increase in halal tourism in 2018, and the rate increased to 42 in 2020. It can be construed that halal tourism draws in a variety of research areas. Indonesian scholars have been contributed to 26.42% of the overall publications, which leading 34 other countries. Authors’ keywords of ‘Islamic tourism’, ‘Muslim tourists’, ‘halal hospitality’, ‘customer satisfaction’, ‘halal certification’ and ‘religiosity’ have substantially impacted the online search for information. This bibliometric study offers a comprehensive and in-depth glance at halal tourism, which could aid industry and academia.
Implant infections after breast reconstruction surgery following mastectomy: Experience from a Greek breast unit
Authors: Michalopoulos Nikolaos V. , Frountzas Maximos , Karathanasis Panagiotis , Theodoropoulos Charalampos , Kolia Aikaterini , Zografos Constantinos G. , Triantafyllou Tania , Larentzakis Andreas , Danias Nikolaos , Zografos George C. 
Published: July 2021

BACKGROUND: Despite the dominance of implant-based breast reconstruction after mastectomy, during recent years, it has been correlated to some complications. The aim of this study is to present the Greek experience about management of implant infections after breast reconstructions and to investigate the relationship between possible risk factors and breast pocket fluid cultures. METHODS: In total, 260 patients underwent implant-based breast reconstruction due to breast cancer in our center from 2016 until 2020. 46 patients, that underwent implant or expander replacement after breast reconstruction due to mastectomy were included in the present study. RESULTS: 260 patients underwent breast reconstruction in our center and in 46 (18%) of them an implant replacement was required. 21 patients (8%) presented clinically with an implant infection, but 12 of them (5%) had positive cultures from the breast pocket fluid. On the contrary, 25 patients (10%) presented no clinical signs of implant infection, but 5 of them (2%) had a positive culture. In addition, we demonstrated a correlation between implant infection and positive cultures (p = 0.009), along with an association between chemotherapy before implant placements and negative cultures (p = 0.035). Finally, the most common pathogen was Staphylococcus epidermidis (29%), followed by Escherichia coli (24%) and Staphylococcus aureus (18%). CONCLUSION: Implant infection still remains a very serious complication after breast reconstruction surgery. The establishment of a therapeutic protocol, with specific antimicrobial and surgical targets seems as an effective strategy against implant infections.
CD26 expression on donor harvest as a risk predictive biomarker for developing graft-versus-host disease post-allogeneic hematopoietic stem cell transplantation: A ten-year follow-up study
Authors: Punatar Sachin , Kandekar Shruti , Khattry Navin , Gokarn Anant , Prabhash Kumar , Bakshi Ashish , Rane Pallavi , Mathew Libin , Chiplunkar Shubhada , Kode Jyoti 
Published: July 2021

BACKGROUND: Allogeneic hematopoietic stem cell transplantation (ASCT) is the preferred treatment option for patients with several hematologic disorders and immunodeficiency syndromes. Graft-versus-host disease (GVHD) is an immune mediated post-transplant complication which has a major impact on long-term transplant outcomes. OBJECTIVE: Current efforts are focused on identification of new markers that serve as potential predictors of GVHD and other post-transplant clinical outcomes. METHODS: This study includes donor harvests collected from twenty-three allogeneic donors during period 2008–2009 and respective transplant recipients followed for clinical outcomes till March 2019. Percent CD26+ and CD34+ cells in donor harvest were analyzed using flow cytometry. Percent expression and infused dose of CD26+ and CD34+ cells were evaluated for association with various clinical outcomes. RESULTS: Total 23 healthy donors with median age of 28 years (13 males), and transplant recipients with median age of 24 years (17 males) formed the study cohort. The diagnosis included malignant (n= 13) and non-malignant (n= 10) hematological disorders. Median CD34brCD45lo HSC expression was 0.57% (IQR 0.24–1.03) while median CD26 expression was 19.64% (IQR 8.96–33.56) of all nucleated cells. CD26 expression was associated with donor age (P= 0.037). CD26 percent expression correlated with WBC engraftment (P= 0.015) and with acute GVHD (P= 0.023) whereas infused CD26 cell dose correlated with WBC engraftment (P= 0.004) and risk of CMV reactivation (P= 0.020). There was no statistically significant correlation of either CD26 expression or cell dose with chronic GVHD, EFS or OS. CONCLUSIONS: Our findings suggest a role of CD26 expression on human donor harvest as a potential predictor of acute GVHD. This association warrants further exploration.
Correlation based malicious traffic analysis system
Authors: Arivudainambi D. , Varun Kumar K.A. , Satapathy Suresh Chandra 
Published: July 2021

Artificial intelligence methods have often been applied to carry out specific functions or errands in the cyber-defense realm. However, as adversary methods become more complex and difficult to divine, piecemeal efforts to understand cyber-attacks, and malware-based attacks in particular, are not providing sufficient means for malware analysts to understand the past, present and future distinctiveness of malware. Because, most of the malware communications take place-utilizing services. These services are completely anonymous and monitoring such services is a hard task. To address this issue, this paper proposes a novel traffic analysis scheme using correlation methods (non-parametric approach). Experiments are performed to validate the proposed approach on the real time traffic data collected over the period of 1 week. The experimental results confirm that the proposed method outperforms the existing state of the art traffic analysis schemes. The result also exhibits the traffic classification performance, which is analyzed by the decade old nearest neighbor method.
Enseñar y aprender en la no presencialidad: percepciones de estudiantes de posgrado-docentes
Authors: Cariaga Romina 
Published: July 2021

Este trabajo tiene por objetivo hacer un análisis contextualizado de los relatos de experiencia manifiestos en un foro sobre las prácticas educativas en modalidad no presencial durante la pandemia de docentes-estudiantes de posgrado. Se apoya en herramientas de la investigación cualitativa interpretativa y propone un análisis de los relatos desde dos dimensiones: dificultades encontradas y cambios implementados. Los resultados muestran que si bien los recursos tecnológicos adoptados en este contexto excepcional no reemplazan la potencialidad de los vínculos ni los métodos de la presencialidad se constituyen en recursos necesarios y enriquecedores de todas las propuestas áulicas. Asimismo, se concluye que es necesario pensar en nuevas formas de escolaridad, híbridas, donde lo virtual y lo real coexistan y donde las instituciones procuren la formación de sujetos con capacidades para desenvolverse en entornos cambiantes, dispuestos a aprender de otros y con otros a través de una adopción crítica de la tecnológica.
Teknik pembelajaran bahasa Inggris yang menyenangkan untuk taman kanak-kanak
Authors: Fitriani Somariah , Ilyas Hamzah Puadi 
Published: July 2021

The variety of learning by applying teaching techniques in a fun, effective and efficient manner provides an important role in the learning process of children. Therefore, this community service activity aims to train the participants' abilities in teaching techniques that are specialized in games, songs and storytelling in English. This activity was carried out by using Google Meeting for two days. In addition, the uses of learning media and class routine activities are the goal of this community service. 18 participants participated in this activity, most of whom were kindergarten teachers. The implementation was carried out using the methods of lecturing, brainstorming and discussion, demonstration and mentoring. The results show that the participants' knowledge and understanding of the given themes increased. Participants are also able to answer several questions at the end of the session and be involved in online game activities. The results of the evaluation show that this activity is very useful and beneficial in increasing their ability to implement more varied teaching techniques that are in accordance with the abilities of kindergarten children.
IL-10 and IL-12 (P70) Levels Predict the Risk of Covid-19 Progression in Hypertensive Patients: Insights From the BRACE-CORONA Trial
Authors: Moll-Bernardes Renata , de Sousa Andrea Silvestre , Macedo Ariane V. S. , Lopes Renato D. , Vera Narendra , Maia Luciana C. R. , Feldman André , Arruda Guilherme D. A. S. , Castro Mauro J. C. , Pimentel-Coelho Pedro M. , de Albuquerque Denílson C. , de Paula Thiago Ceccatto , Furquim Thyago A. B. , Loures Vitor A. , Giusti Karla G. D. , de Oliveira Nathália M. , De Luca Fábio A. , Kotsugai Marisol D. M. , Domiciano Rafael A. M. , Santos Mayara Fraga , de Souza Olga Ferreira , Bozza Fernando A. , Luiz Ronir Raggio , Medei Emiliano 
Published: July 2021

Background: Cardiovascular comorbidities such as hypertension and inflammatory response dysregulation are associated with worse COVID-19 prognoses. Different cytokines have been proposed to play vital pathophysiological roles in COVID-19 progression, but appropriate prognostic biomarkers remain lacking. We hypothesized that the combination of immunological and clinical variables at admission could predict the clinical progression of COVID-19 in hypertensive patients.Methods: The levels of biomarkers, including C-reactive protein, lymphocytes, monocytes, and a panel of 29 cytokines, were measured in blood samples from 167 hypertensive patients included in the BRACE-CORONA trial. The primary outcome was the highest score during hospitalization on the modified WHO Ordinal Scale for Clinical Improvement. The probability of progression to severe disease was estimated using a logistic regression model that included clinical variables and biomarkers associated significantly with the primary outcome.Results: During hospitalization, 13 (7.8%) patients showed progression to more severe forms of COVID-19, including three deaths. Obesity, diabetes, oxygen saturation, lung involvement on computed tomography examination, the C-reactive protein level, levels of 15 cytokines, and lymphopenia on admission were associated with progression to severe COVID-19. Elevated levels of interleukin-10 and interleukin-12 (p70) combined with two or three of the abovementioned clinical comorbidities were associated strongly with progression to severe COVID-19. The risk of progression to severe disease reached 97.5% in the presence of the five variables included in our model.Conclusions: This study demonstrated that interleukin-10 and interleukin-12 (p70) levels, in combination with clinical variables, at hospital admission are key biomarkers associated with an increased risk of disease progression in hypertensive patients with COVID-19.
Optical Stimulation Luminescence Dating of Deltas Revealed the Early to Mid-Holocene Lake-level Fluctuations of Daihai, Inner Mongolia, Northern China
Authors: Huang Shixin , Chun Xi 
Published: July 2021

Lake-level reconstruction of inland enclosed lakes especially for monsoon-sensitive areas is of great significance to reveal regional climate changes. Daihai, a typical enclosed lake at the marginal of the East Asian summer monsoon (EASM) area in north China, is sensitive to climate changes due to its unique regional characteristics. There were a series of lakeshore terraces, highstand lacustrine sediments, and braided river deltas, providing sufficient geomorphologic and stratigraphic evidence for the reconstruction of lake-level fluctuations of Daihai. Reconstructed lake-level variations during the early and mid-Holocene were constructed based on 22 quartz optical stimulated luminescence (OSL) ages from six well-preserved profiles around Daihai Basin. Our results indicated Daihai showed a relatively low level at 10.2 ka, and a gradually increasing lake level following the enhanced monsoon precipitation during the mid-Holocene. Specifically, the high lake level began to develop at 8.1 ka and reached the maximum at 5.2 ka, with ∼40 m higher than present. At this time, the lake area expanded to ∼400 km2, approximately six times as large as that of present, corresponding to the maximum monsoon precipitation and intensity of EASM during the mid-Holocene. However, our stratigraphic records showed a part of the depositional records in the north and east of the Daihai was missed after 5.2 ka, probably indicating a sudden drop of the Daihai lake level. These rapid level fluctuations were likely to be interpreted by some local scenarios and need to be further investigated in the future. Overall, the lake-level fluctuation of Daihai during the early and mid-Holocene was slightly different from that observed in the previously published regional records. Possibly, the interaction of the EASM and regional feedback from topography, and hydrology factors might have contributed to the spatial complexity and distinction.
Congenital Deficiency of Conventional Dendritic Cells Promotes the Development of Atopic Dermatitis-Like Inflammation
Authors: Nishikawa Yotaro , Fukaya Tomohiro , Fukui Takehito , Uto Tomofumi , Takagi Hideaki , Nasu Junta , Miyanaga Noriaki , Riethmacher Dieter , Choijookhuu Narantsog , Hishikawa Yoshitaka , Amano Masahiro , Sato Katsuaki 
Published: July 2021

Atopic dermatitis (AD) is a common pruritic inflammatory skin disease characterized by impaired epidermal barrier function and dysregulation of Thelper-2 (TH2)-biased immune responses. While the lineage of conventional dendritic cells (cDCs) are implicated to play decisive roles in T-cell immune responses, their requirement for the development of AD remains elusive. Here, we describe the impact of the constitutive loss of cDCs on the progression of AD-like inflammation by using binary transgenic (Tg) mice that constitutively lacked CD11chi cDCs. Unexpectedly, the congenital deficiency of cDCs not only exacerbates the pathogenesis of AD-like inflammation but also elicits immune abnormalities with the increased composition and function of granulocytes and group 2 innate lymphoid cells (ILC2) as well as B cells possibly mediated through the breakdown of the Fms-related tyrosine kinase 3 ligand (Flt3L)-mediated homeostatic feedback loop. Furthermore, the constitutive loss of cDCs accelerates skin colonization of Staphylococcus aureus (S. aureus), that associated with disease flare. Thus, cDCs maintains immune homeostasis to prevent the occurrence of immune abnormalities to maintain the functional skin barrier for mitigating AD flare.
Characteristics of Peripheral Lymphocyte Subsets in Patients With Acute-On-Chronic Liver Failure Associated With Hepatitis B
Authors: Li Juan , Hu Chun-Hua , Chen Yi , Zhou Mi-Mi , Gao Zhi-Jie , Fu Meng-Jun , Wang Jing , Li Jian-Zhou , Chen Tian-Yan , Zhao Ying-Ren , He Ying-Li 
Published: July 2021

Background and Aims: Acute-on-chronic liver failure (ACLF) is a rare, but dramatic clinical syndrome. There is substantial evidence suggesting that immunity-mediated inflammation plays an important role in HBV-ACLF. Our aim was to characterize the proportion and cell counts of peripheral blood lymphocyte subsets in acute-on-chronic liver failure patients caused by HBV infection.Methods: One hundred and seventeen patients were enrolled in this study, including those with HBV-related ACLF (HBV-ACLF; n = 70), and HBV related non-ACLF patients (HBV non-ACLF; n = 47). Demographics, clinical and laboratory data at hospital admission were retrospectively analyzed. The percentage and cell count of peripheral lymphocyte subsets were evaluated by flow cytometry. Comparison analysis was performed by t-test or non-parametric Mann–Whitney U-test. Actuarial probabilities of death were calculated by the Kaplan-Meier method.Results: Both circulating lymphocyte count and lymphocyte percentage were significantly reduced in patients with HBV-ACLF (P &lt; 0.001). The CD8+ T cell, CD4+ T cell, and CD16+CD56+ NK cell counts were significantly decreased in HBV-ACLF. Consistently, flow cytometric analysis showed that CD8+ T cell counts were significantly decreased in non-survivors, while no significant differences were found in CD4+ T cell, CD19+ B cell, or CD56+CD16+ NK cell counts. Furthermore, the group with the lower CD8+ T cell count displayed a significantly higher mortality rate compared with the group with the higher CD8+ T cell count.Conclusions: The abnormal prevalence of lymphocyte subsets may be important in the pathogenesis of HBV-ACLF. The decrease in CD8+ T cell counts may be related to poor survival in HBV-ACLF patients.
Regulation of Tumor Necrosis Factor-α by Peptide Lv in Bone Marrow Macrophages and Synovium
Authors: Mukai Manabu , Uchida Kentaro , Okubo Tadashi , Takano Shotaro , Matsumoto Toshihide , Satoh Masashi , Inoue Gen , Takaso Masashi 
Published: July 2021

Background:Bone marrow-derived monocytes/macrophages are recruited into synovial tissue, where they contribute to synovial inflammation in osteoarthritis through inflammatory cytokine production. Recent studies have suggested that V-Set and transmembrane domain-containing 4 (VSTM4) and its fragment, peptide Lv, exhibit immunosuppressive activity on T cells and vascular endothelial growth factor (VEGF)-like activity, respectively. Given that evidence suggests that VEGF may play a role in macrophage function, we investigated peptide Lv-mediated regulation of inflammatory cytokines in bone marrow macrophages (BMMs) and synovial inflammation.Method:To investigate the effects of peptide Lv, BMMs were stimulated with vehicle, LPS, or LPS + peptide Lv, andTnfa, Il1b, Il6, andIfngexpression were evaluated using quantitative PCR (qPCR). TNF-α and IFN-γ production was measured using ELISA. To examine the effect of peptide Lv deficiency on macrophages and synovitis,peptide Lv-deficient mice were generated using genome editing. LPS-inducedTnfaandIfngexpression and TNF-α and IFN-γ production were evaluated in BMM isolated from wild-type andpeptide Lv-deficient mice. Additionally,TnfaandIfngexpression levels were compared between wild-type andpeptide Lv-deficient mice before and after knee injury.Results:Peptide Lv suppressed the LPS-mediated elevation in TNF-α and IFN-γ. LPS stimulation significantly increased TNF-α and IFN-γ production in BMM derived frompeptide Lv-deficient mice compared to wild-type mice. Synovial TNF-α expression in the injured knee was elevated inpeptide Lv-deficient compared to wild-type mice.Conclusion:Peptide Lv suppressed TNF-α in macrophages and plays a role in synovial inflammation. Thus, peptide Lv may be a useful therapeutic target for synovitis.
Antimicrobial Resistance Profiling and Phylogenetic Analysis of Neisseria gonorrhoeae Clinical Isolates From Kenya in a Resource-Limited Setting
Authors: Juma Meshack , Sankaradoss Arun , Ndombi Redcliff , Mwaura Patrick , Damodar Tina , Nazir Junaid , Pandit Awadhesh , Khurana Rupsy , Masika Moses , Chirchir Ruth , Gachie John , Krishna Sudhir , Sowdhamini Ramanathan , Anzala Omu , Meenakshi Iyer S. 
Published: July 2021

BackgroundAfrica has one of the highest incidences of gonorrhea. Neisseria gonorrhoeae is gaining resistance to most of the available antibiotics, compromising treatment across the world. Whole-genome sequencing (WGS) is an efficient way of predicting AMR determinants and their spread in the population. Recent advances in next-generation sequencing technologies like Oxford Nanopore Technology (ONT) have helped in the generation of longer reads of DNA in a shorter duration with lower cost. Increasing accuracy of base-calling algorithms, high throughput, error-correction strategies, and ease of using the mobile sequencer MinION in remote areas lead to its adoption for routine microbial genome sequencing. To investigate whether MinION-only sequencing is sufficient for WGS and downstream analysis in resource-limited settings, we sequenced the genomes of 14 suspected N. gonorrhoeae isolates from Nairobi, Kenya.MethodsUsing WGS, the isolates were confirmed to be cases of N. gonorrhoeae (n = 9), and there were three co-occurrences of N. gonorrhoeae with Moraxella osloensis and N. meningitidis (n = 2). N. meningitidis has been implicated in sexually transmitted infections in recent years. The near-complete N. gonorrhoeae genomes (n = 10) were analyzed further for mutations/factors causing AMR using an in-house database of mutations curated from the literature.ResultsWe observe that ciprofloxacin resistance is associated with multiple mutations in both gyrA and parC. Mutations conferring tetracycline (rpsJ) and sulfonamide (folP) resistance and plasmids encoding beta-lactamase were seen in all the strains, and tet(M)-containing plasmids were identified in nine strains. Phylogenetic analysis clustered the 10 isolates into clades containing previously sequenced genomes from Kenya and countries across the world. Based on homology modeling of AMR targets, we see that the mutations in GyrA and ParC disrupt the hydrogen bonding with quinolone drugs and mutations in FolP may affect interaction with the antibiotic.ConclusionHere, we demonstrate the utility of mobile DNA sequencing technology in producing a consensus genome for sequence typing and detection of genetic determinants of AMR. The workflow followed in the study, including AMR mutation dataset creation and the genome identification, assembly, and analysis, can be used for any clinical isolate. Further studies are required to determine the utility of real-time sequencing in outbreak investigations, diagnosis, and management of infections, especially in resource-limited settings.