bluuebunny/crossref_metadata_2025_split huggingface dataset:
lncRNA XIST knockdown suppresses hypoxia/reoxygenation (H/R)‐induced apoptosis of H9C2 cells by regulating miR‐545‐3p/G3BP2
Authors: Xiao Xiaohong , He Zhenzhen , Tong Suiyang , Dai Lixia , Xiao Qiuling , Qin Zhongxin , Lin Tao 
Published: June 2021

AbstractThis study was aimed at determining the roles and functions of lncRNA XIST/miR‐545‐3p/G3BP2 axis during hypoxia/reoxygenation (H/R)‐induced H9C2 cell apoptosis. H9C2 cells were distributed into two groups, the H/R injury and control groups. High‐throughput lncRNA sequencing was applied in the determination of differentially expressed lncRNAs between H/R‐induced H9C2 cells and normal H9C2 cells. Real‐time polymerase chain reactions (RT‐PCR) were used to confirm the expression levels of lncRNA XIST in H/R‐induced H9C2 cells. H9C2 cells were then transfected with lncRNA XIST recombinant plasmid (lncRNA XIST), sh‐LINC XIST, agomiR‐545‐3p, antagomiR‐545‐3p, pcDNA‐G3BP2, sh‐G3BP2, and a corresponding negative control (NC). Bioinformatic analyses revealed that MiR‐545‐3p was a target for lncRNA XIST. This finding was confirmed by dual‐luciferase reporter assay. The degree of cell apoptosis was evaluated by a flow cytometer. RT‐PCR and western blot were performed to assess the apoptotic‐related proteins in each group. A total of 859 differentially expressed lncRNAs (up‐regulated = 502, down‐regulated = 357) were identified. LncRNA XIST was found to be down‐regulated in H/R‐induced H9C2 cells while miR‐545‐3p was distinctly up‐regulated. miR‐545‐3p was established to be a direct target for LncRNA XIST. LncRNA XIST significantly enhanced the apoptotic rate, while its inhibition suppressed the apoptotic rate. AgomiR‐545‐3p partially blocked the lncRNA XIST and enhanced the apoptosis of H/R‐induced H9C2 cells. Moreover, miR‐545‐3p was shown to be a direct target for G3BP2. The overexpression of G3BP2 partially reversed the apoptotic effects of miR‐545‐3p on H/R‐induced H9C2 cells. lncRNA XIST/miR‐545‐3p/GBP2 was found to be an apoptotic regulator in H/R‐induced H9C2 cells.
Decellularized kidney scaffold alters the healing response in chronic renal failure
Authors: Wang Fangfang , Liu Xiaolin , Yu Yaling , Zhang Rui , Li Miaozhong , Ding Yuqiang , Xi Shanshan , Jiang Junqun , Chen Chuan , Wang Zhibin , Wei Peng , Mei Jin 
Published: June 2021

AbstractDecellularized (DC) kidney scaffold shows great potential for renal recovering. Our study explored the effect of the DC kidney scaffolds treating on chronic renal failure (CRF) through grafting them on 5/6 nephrectomized (5/6 Nx) rat kidneys compared with gelatin sponges covered the incision edges. Blood urea nitrogen and angiotensin II were significantly lower in most time in scaffold‐grafted groups. Remnant kidney tumor necrosis factor‐α and fibroblast growth factor in scaffold‐grafted groups significantly reduced in majority of time points compared with controls. But platelet‐derived growth factor‐BB showed a different varied tendency, first higher in scaffold groups on week 2, 4, 6, but lower on week 8, finally no difference on week 12 compared with gelatin‐sponge groups. In addition, the index of glomerular sclerosis was significantly lesser in scaffold‐grafted groups, and, the accumulation of collagen III and collagen IV decreased in scaffold‐grafted groups on week 6, 8, 12 compared with gelatin‐sponge groups. Moreover, DC scaffolds enhanced the expression of CD133 on week 2, 6, 8, 12. In conclusion, DC kidney scaffold altered the healing response after 5/6 nephrectomy and ameliorated renal injury to some degree. Therefore, DC kidney scaffold could be a promising therapeutic method on CRF.
Relative expression of proinflammatory molecules in COVID‐19 patients who manifested disease severities
Authors: Nigar Shireen , Shah SM Tanjil , Setu Md Ali Ahasan , Dip Sourav Dutta , Ibnat Habiba , Islam M. Touhidul , Akter Selina , Jahid Iqbal Kabir , Hossain M. Anwar 
Published: June 2021

AbstractAggressive immune response, due to overexpressed proinflammatory molecules, has been characterized in coronavirus disease 2019 (COVID‐19) patients. Some of those mediators have a dual and opposite role on immune systems at play behind differential disease severities. We investigated the expression of some cytokines and chemokines in COVID‐19 patients in Bangladesh. We diagnosed the patients by detecting severe acute respiratory syndrome coronavirus 2 RNA in nasal swab samples by the real‐time RT‐PCR method. Thirty adult patients were preselected based on their disease severities and grouped into mild, moderate, and severe cases. Nine healthy volunteers participated in this study as a control. Relative expression of nine cytokines/chemokine in total leukocytes was semi‐quantified in SYBRgreen‐based real‐time quantitative reverse‐transcriptase polymerase chain reaction. We performed statistical tests on transformed log data using SPSS 24.0. At the onset of symptoms (Day 1), angiotensin‐converting enzyme 2 (ACE2) (p < 0.05) and interleukin (IL)‐6 (p > 0.05) were upregulated in all COVID‐19 groups, although the expression levels did not significantly correlate with disease severities. However, expressions of IL‐6, monocyte chemotactic protein‐1, macrophage inflammatory protein‐1α, tumor necrosis factor‐α (TNF‐α), RANTES (regulated upon activation, normal T cell expressed and secreted), and ACE2, on Day 14, were positively correlated with disease severities. Relative viral load at Day 1 showed no significant correlation with cytokine expression but had a significant positive correlation with RANTES and ACE2 expression on Day 14 (p < 0.05). Male patients had a higher level of IL‐6 than female patients on Day 1 (p < 0.05). All COVID‐19 patients showed upregulated cytokines and chemokines on Day 14 compared to Day 1 except TNF‐α. Female patients had a higher expression of ACE2 and IL‐12 on Day 14. Upregulated cytokines/chemokines at the convalescent stage, especially IL‐6, may help in targeting anticytokine therapy in post‐COVID‐19 patients' management.
Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods
Authors: Emmerich Petra , von Possel Ronald , Hemmer Christoph Josef , Fritzsche Carlos , Geerdes‐Fenge Hilte , Menge Babett , Messing Claudia , Borchardt‐Lohölter Viola , Deschermeier Christina , Steinhagen Katja 
Published: June 2021

AbstractSerological testing for anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antibodies is used to detect ongoing or past SARS‐CoV‐2 infections. To study the kinetics of anti‐SARS‐CoV‐2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction‐confirmed coronavirus disease 2019 (COVID‐19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS‐CoV‐2‐infected cells. They were further tested for antibodies against the S1 domain of the SARS‐CoV‐2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme‐linked immunosorbent assays. The assay specificities were 94.4%–100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS‐CoV‐2 as substrate and provided information on whether or not the individual has been infected with SARS‐CoV‐2. Enzyme‐linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS‐CoV‐2.
Inhibitory effect on SARS‐CoV‐2 infection of neferine by blocking Ca2+‐dependent membrane fusion
Authors: Yang Yang , Yang Peng , Huang Cong , Wu Yuming , Zhou Zhe , Wang Xuejun , Wang Shengqi 
Published: June 2021

AbstractThe coronavirus disease 2019 (COVID‐19) pandemic has focused attention on the need to develop effective therapeutics against the causative pathogen, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), and also against other pathogenic coronaviruses. In this study, we report on a kind of bisbenzylisoquinoline alkaloid, neferine, as a pan‐coronavirus entry inhibitor. Neferine effectively protected HEK293/hACE2 and HuH7 cell lines from infection by different coronaviruses pseudovirus particles (SARS‐CoV‐2, SARS‐CoV‐2 [D614G, N501Y/D614G, 501Y.V1, 501Y.V2, 501Y.V3 variants], SARS‐CoV, MERS‐CoV) in vitro, with median effect concentration (EC50) of 0.13–0.41 μM. Neferine blocked host calcium channels, thus inhibiting Ca2+‐dependent membrane fusion and suppressing virus entry. This study provides experimental data to support the fact that neferine may be a promising lead for pan‐coronaviruses therapeutic drug development.
Sophorolipid Reduces Bitter Taste in Humans In Vivo and In Vitro
Authors: Ozdener Mehmet Hakan , Spielman Andrew I. , Wise Paul M. 
Published: June 2021

AbstractBitter taste warns us against ingesting toxic chemicals but can also discourage consumption of healthful nutrients and prescribed medications. Thus, discovery of ingredients to reduce bitterness is an important research priority. The potential bitter‐taste‐blocking effect of sophorolipids has recently been reported. Sophorolipids are biosurfactant glycolipids normally synthesized via yeast fermentation. In the current experiments, the effect of sophorolipid on bitter taste was evaluated using both cultured human taste papillae (HBO) cells and in vivo sensory experiments with human tasters. Sophorolipids significantly reduced responses of HBO cells to a mixture of diverse bitter compounds. Human participants rated the bitterness of a mixture of diverse bitter compounds as less intensely bitter both after prerinsing with a sophorolipid solution and when sophorolipids were added to the mixture. Taken together, these results suggest that sophorolipids may reduce perceived bitterness in humans, at least in part by acting on peripheral mechanisms. Although further work is needed to confirm these findings and determine the exact mechanism(s) of action, thus far sophorolipids show promise as candidate ingredients to reduce bitterness.
Use of the abrasion technique in minimal processing as an alternative to increase purchase acceptability and minimize browning in yam
Authors: dos Santos Morais Maria Aparecida , Fonseca Kelem Silva , de Medeiros Rosilene Alves , de Andrada Lucas Vinícius Pierre , Saraiva Rogério de Aquino , Ferreira‐Silva Sérgio Luiz , Alves de Lima André Luiz , do Nascimento Simões Adriano 
Published: June 2021

AbstractBACKGROUNDThe present study investigated the sensory acceptance, oxidative damage and protection, and possible anatomical‐structural damage of cells from the surface of shapes of minimally processed yam. The tubers were minimally processed into thepeeled rondelle,diceand ‘chateau cut’ (chateau) shapes, the latter of which was obtained after performing the abrasion technique. Control treatment corresponded to the rondelle shape with the periderm. The pieces were kept packed at 5 ± 2 °C for 14 days.RESULTSPeeled rondelleandchateauwere sensorially the most well‐accepted yam shapes and achieved the highest purchase intention. The enzymes were partially modulated by the detected H2O2levels. Oxidative burst lasted longer in the minimally processed tissues than in the control. Polyphenol oxidase activity showed a clear difference in behavior between the minimally processed pieces and the control. Minimal processing induced transient increases in phenolic compounds, for which the expression was lowest in the abraded pieces. On the other hand, these pieces exhibited greater cell collapse on the surface of the amyliferous parenchyma.CONCLUSIONBased on the results of the trained panel, the abrasion technique is an alternative to provide shapes that are better accepted and marketable, more resistant to browning, and can be stored for up to 12 days. Resistance to browning may be related to a more efficient modulation of enzymatic antioxidant systems and intense deposition of cell debris on the surface of the amyliferous parenchyma. © 2021 Society of Chemical Industry.
Propionate and Butyrate Produced by Gut Microbiota after Probiotic Supplementation Attenuate Lung Metastasis of Melanoma Cells in Mice
Authors: Chen Lili , Zhou Xinyu , Wang Yawei , Wang Dake , Ke Yueshuang , Zeng Xianlu 
Published: June 2021

ScopeThe beneficial effects of probiotics in reducing gastrointestinal inflammation and in preventing colorectal cancer have been reported, but the mechanism underlying the immunomodulatory effect of probiotics in inhibiting extra‐intestinal tumor progression remains unclear.Methods and ResultsThis study shows that probiotic supplementation attenuate lung metastasis of melanoma cells in mice. Feeding mice with VSL#3 probiotics change the composition and proportion of gut microbiota. The changes in gut bacteria composition, such as in the abundance of Lachnospiraceae, Streptococcus, and Lachnoclostridium, are associated with the production of short‐chain fatty acids in the gut. The concentrations of propionate and butyrate are upregulated in gut and blood after feeding VSL#3, and the increase in propionate and butyrate levels promotes the expression of chemokine (C‐C motif) ligand 20 (CCL20) in lung endothelial cells and the recruitment of T helper 17 (Th17) cells to the lungs via the CCL20/chemokine receptor 6 axis. The recruitment of Th17 cells decreases the number of tumor foci in lungs and attenuates the lung metastasis of melanoma cells in mice.ConclusionsThe results provide new information on the role and mechanisms of action of probiotics in attenuating extra‐intestinal tumor metastasis.
Trendbericht Technische Chemie 2021
Authors: de Haart Bert , Fantz Ursel , Hecimovic Ante , Schulz Andreas , Navarrete Munoz Alexander , Klumpp Michael 
Published: June 2021

AbstractUm das Energiesystem nachhaltig zu machen, ist mehr erneuerbarer elektrischer Strom nötig. Er muss zudem für bislang stromentkoppelte Energiesektoren technisch nutzbar werden. Dementsprechend gibt es immer mehr Verfahren, die elektrische Energie in andere Energieformen umwandeln. Beispiele sind Hochtemperatur‐Ko‐Elektrolyse, Plasmaprozesse zur Aktivierung von H2O und CO2 sowie daran anschließende Synthesen von CH‐basierten Energieträgern.
Transvesical versus extravesical approach to laparoscopic posthysterectomy vesicovaginal fistula repair: A retrospective study from two medical centers
Authors: Zhou Peng , Deng Wen , Li Junhua , Pan Huixian , Wang Yanbin , Song Chen , Liu Weipeng , Fu Bin 
Published: June 2021

AbstractBackgroundThe controversy on the best surgical approach for vesicovaginal fistula (VVF) repair remains due to the scarcity of high‐level evidences. We aim to analyze the efficacy and safety of the laparoscopic transvesical (LT) and laparoscopic extravesical (LE) approaches to posthysterectomy VVF (PH‐VVF).MethodsData of 64 patients with PH‐VVFs who were laparoscopicly treated in the First Affiliated Hospital of Nanchang University and the Hangzhou Third Hospital from January 2011 to November 2019 were retrospectively collected. The operative time (OT), estimated bleeding volume (EBV), postoperative bladder function and complications, hospital stay length (HSL), surgery success rate, and recurrence were compared between the two groups.ResultsIn all, there were no significant differences in baseline characteristics between those two arms. Both approaches were successfully performed without open conversion in either group. The LT group was significantly corrected with a shorter mean OT than the LE group (p < 0.001), regardless of the fistula's position. No significant differences existed in the mean EBV and HSL between the two interventions (p = 0.136 and p = 0.210, respectively). The tendency of postoperative complications and success rates of surgery were also comparable in both groups. The patients in the LT group had similar bladder functions to those in LE group. The recurrence occurred in one patient in each arm during the follow‐up periods of 12–36 months.ConclusionsThe LT approach is significantly related to a shorter OT than the LE approach without compromising the safety and success rates in repairing PH‐VVF.
Synthesis, structure, Hirshfeld surface, DFT, and molecular docking studies of a new organic cocrystal: creatinine:2,3‐pyridinedicarboxylic acid
Authors: Matheswari P. Pon , Asha R. Nandini , Bhuvanesh Nattamai , Nayagam B. Ravindran Durai 
Published: June 2021

AbstractA new cocrystal (CR:2,3PDCA) of creatinine (CR) with 2,3‐pyridine dicarboxylic acid (2,3PDCA) was grown by slow evaporation method, and its crystal structure was characterized by single‐crystal X‐ray diffraction (SCXRD) analysis. The cocrystal was found to be triclinic with space group P‐1 and lattice parameters a = 7.3610(7) Å, b = 8.7813(9) Å, and C = 9.4173(10) Å. The intermolecular hydrogen bonds, namely, N‐H…N and C‐O…H‐N interactions, together play a major role in stabilizing the cocrystal. Further, the molecular interactions in the crystal structure were analyzed by considering short contacts and intermolecular contactsusing Hirshfeld surface methods. Furthermore, the coordinates of cocrystal were obtained by DFT calculations using the B3LYP method with a 6‐311++G (d,p) basis set. Besides this, other molecular properties such as frontier molecular orbital, HOMO‐LUMO energies, and Mulliken charge analysis were also reported. Furthermore, a molecular docking study was performed to study the anti‐inflammatory property of the cocrystal using the Auto‐Dock vina program by investigating the binding patterns of the synthesized cocrystal into a 1R35 inhibitor. In vitro anti‐microbial activities against some bacterial and fungal strains were also investigated.
Quality retention and shelf‐life prolongation of cucumbers (Cucumis sativus L.) under different cool storage systems with passive modified atmosphere bulk packaging
Authors: Kaur Preetinder , Devgan Kirandeep , Kumar Nitin , Kaur Amrit , Kumar Mahesh , Sandhu Kanchan 
Published: June 2021

AbstractStorage plays an important role in maintaining the post‐harvest shelf life of fresh fruits and vegetables. Cool storage of perishables is costly due to higher energy input. So, to reduce the operating cost and minimize the postharvest loss, a low‐cost on‐farm storage structure is required, especially in tropical developing nations. In this study, the effectiveness of different cool storage systems was validated by conducting shelf‐life studies on freshly harvested seedless cucumbers with (LDPE bags; 37.5‐μm thickness) and without packaging (open plastic crates) under ambient (25.0 ± 8°C; 55 ± 14% RH), cool storage (10.0°C; 90 ± 5% RH), and evaporative cool storage (ECS) systems (19 ± 3°C; 82.0 ± 9% RH). The shelf life and quality of cucumbers were determined based on weight loss, titratable acidity, total soluble solids, ascorbic acid, firmness, and total colour difference. Analysis of the data revealed that the effect of storage conditions and packaging on shelf life and quality was significant (p ≤ 0.05). It was found that the samples packaged in LDPE films had better firmness and lower weight loss as compared with unpackaged samples. Adoption of the evaporative cooling system helped in achieving temperatures of 8–10°C lower than the ambient depending on the environmental parameters. The relative humidity of the evaporative cooling system ranged from 75% to 90%, which is highly suited for the storage of fruits and vegetables. Although the shelf life of cucumbers was longer under cool storage (24 days), ECS could be used as an alternative for low‐cost on‐farm short‐term storage of fresh fruits and vegetables before marketing.
Growth, crystal structure, thermal, spectral studies and density functional theory computational analysis of an organic nonlinear optical crystal: 2‐{3‐[2‐(4‐dimethylaminophenyl)vinyl]‐5,5‐dimethylcyclohex‐2‐enylidene}‐malonitrile
Authors: Boomadevi Shanmugam , Ramachandran Mohanraj , Balamurugan Karuppannan , Nayak Dipali , Thangavel Rajalingam , Pandiyan Krishnamoorthy , Anandan Sambandam , Sastikumar Dilibabu 
Published: June 2021

AbstractWe report on synthesis, growth and characterization of an organic nonlinear optical (NLO) crystal 2‐{3‐[2‐(4‐dimethylaminophenylvinyl]‐5,5‐dimethylcyclohex‐2‐enylidene}‐malonitrile (DAT2). The functional groups are identified by 1H‐NMR. Large size single crystal of DAT2 of size 1.2 × 0.5 × 0.2 cm3 is grown for the first time using solution growth technique. The physicochemical properties of DAT2 crystal such as crystal structure, melting point, optical reflectance and photoluminescence are studied experimentally. Density functional theory (DFT) analysis is employed to study the band structure, optical and electronic properties of DAT2. The theoretical bandgap was compared with the experimental one. LDA approximation has been used to compare the GGA results.
Synthesis and In Vitro Anticancer Activities of New 1,4‐Disubstituted‐1,2,3‐triazoles Derivatives through Click Approach
Authors: Nipate Amol S. , Jadhav Chetan K. , Chate Asha V. , Deshmukh Tejshri R. , Sarkate Aniket P. , Gill Charansingh. H. 
Published: June 2021

AbstractThe synthesis of a new series 1‐(3‐methoxy‐4‐((1‐phenyl‐1H‐1,2,3‐triazole‐4‐yl)methoxy) phenyl)ethanone via click chemistry approach utilizing azide‐alkyne cycloaddition reactions is reported in this study. The structures of all newly synthesized compounds were analyzed by IR, NMR, and Mass spectral techniques. All the newly synthesized compounds were subjected to cytotoxicity assay against a panel of three human cancer cell lines (HepG2, A549, and MCF‐7) using 3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), to check in vitro anticancer activity. Compound 5 b was found to be the most potent anticancer agents with IC50 value 3.42 μM, 1.26 μM, and 5.96 μM against HepG2, A549, and MCF‐7 respectively. Among the tested compounds, 5 a and 5 q have shown notable anticancer activity as compared to the reference drug, Adriamycin
Ag@TiO2 as an Efficient Electrocatalyst for N2 Fixation to NH3 under Ambient Conditions
Authors: Li Xue , Cao Yang , Xu Tong , Luo Yonglan , Li Tingshuai , Zhao Haitao , Lu Siyu , Shi Xifeng , Asiri Abdullah M. , Hu Jianming , Liu Qian , Sun Xuping 
Published: June 2021

AbstractWith the use of traditional Haber‐Bosch N2 fixation, a huge amount of CO2 emission bring a huge burden to the environment. Electrochemical N2 reduction provides an environmental‐friendly and sustainable alternative to NH3 synthesis at ambient conditions. Herein, we report our recent experimental finding that Ag composited with TiO2 can greatly improve the N2 reduction reaction performance of TiO2 toward ambient conversion. In 0.1 M Na2SO4 (pH=7), such electrocatalyst achieves a large NH3 yield rate of 14.88 μg h−1 mg−1cat. with a Faradaic efficiency of 6.2 % at −0.70 V vs. reversible hydrogen electrode. Besides, it also demonstrates high electrochemical and structural stability for NH3 generation.