bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autophagy-Sirtuin1(SIRT1) Modulated The Endothelial Progenitor Cells (EPCs) Proliferation and Migration Via wnt/β-Catenin/GSK3β Signaling Pathway and Alleviated The Coronary Atherosclerosis (CAS) in Mice
Authors: Li Yanwei , Cui Wei , Song Bing , Ye Xuying , Li Zhuqing , Lu Chengzhi 
Published: February 2021

Abstract
Background and purpose: SIRT1 exerted its link to CAS risk in humans and EPCs presented its reparative role in CAS. In this study, we explored the role of SIRT1 in CAS mice and also its modulation in EPCs.Methods and materials: ApoE-/- mice were fed with high-fat and high-glucose food to establish the CAS animal model with the normally-raised C57BL/6 mice as a healthy control group. 5 ApoE mice were intravenously injected with SIRT1 activator, SRT 2104 and another 5 were injected with inhibitor Nicotinamide in tail. Weight changes were recorded per week. Blood samples were taken from posterior orbital venous plexus and detected by automatic biochemical analyzer for lipid concentrations. Coronary artery tissues were observed. HE staining displayed the pathological condition while Immunohistochemistry (IHC) evaluated the CD34+/VEGFR2+ relative density. In vitro, EPCs were isolated from bone marrow each group and then purified, cultured and verified using immunofluorescence staining (IFS). Thereafter, we examined the modulatory mechanism of SIRT1 in EPCs by RT-PCR, MTT, Western Blot (WB) and colony formation, scratch methods, connecting the wnt/β-catenin/GSK3β signaling pathway.Results：SIRT1 activation negatively regulated the weight and TC, TG and LDL. SIRT1 activator alleviated the lesion area and decreased the CD34+/VEGFR2+ density which was higher in coronary artery tissues in CAS and SIRT1 inhibitor groups. In vitro, SIRT1 activator promoted the bone marrow-derived EPCs proliferation, migration and activated wnt/β-catenin/GSK3β signaling pathway while inhibited the autophagy biomarkers ATG1 and LC3II. Furthermore, inhibition of autophagy led to the upregulation of SIRT1 and increase in cell proliferation, migration and wnt/β-catenin/GSK3β activity. The suppression of the pathway in turn lowered SIRT1 expression in EPCs, attenuated the cell proliferation and migration and promoted autophagy. Conclusion: Taken all the findings together, this research disclosed that SIRT1 activator might perform its protective role in CAS through autophagy inhibition via wnt/β-catenin/GSK3β signaling pathway in EPCs.
Contamination of Water, Sediment and Fish With Residues of Pesticides Used in Cotton Production in Northern Benin
Authors: Douny Caroline , Zoumenou Y.M. , Aïna Martin , Toko Ibrahim , Igout Ahmed , Guedegba Léa , Chabi Sika , Kestemont Patrick , Scippo Marie-Louise 
Published: February 2021

Abstract
In Northern Benin, insecticides are used for cotton production. These insecticides can be easily transferred to water ponds close to cotton fields. To monitor insecticides levels in water, sediments and fish samples from water ponds, a GC-MS analytical method was developed to detect residues of endosulfan, DDT and its parent compounds, isomers of HCH, pyrethroids and chlorpyrifos. In addition, the influence of storage conditions of water sample on pesticides determination performance has been studied. The limits of quantification were between 0.16 and 0.32 µg/L in water, 0.5 and 1 μg/kg in sediment and 1 and 2 μg/kg in fish. Twenty samples of water, twenty of sediments and forty of fish were taken in four different water reservoirs at five different times. Alpha-endosulfan, lambda-cyhalothrin and permethrin were the identified in sediment while p,p’-DDE, α- and β-HCH, chlorpyrifos, lambda-cyhalothrin and permethrin were detected in fish. Only organochlorines were determined in water because of the lack of recovery of pyrethroids from water stored in glass. Concentrations of insecticide residues in sediment for all water ponds ranged from non-detected to 101 µg/kg and from non-detected to 36 µg/kg in fish. Preliminary risk assessment for consumers of the North of Benin showed that the Estimated Daily Intakes were lower than the Acceptable Daily Intake sand Acute Reference Doses for all consumers. However, as one fish can be contaminated by 5 pesticide residues at the same time, it is not possible to exclude a risk for the consumer due to his exposure to mixtures of pesticides.
AC Conductivity and Conduction Mechanism of Iron (II) Chloride (FeCl2) /Poly (vinyl alcohol) (PVA)/Poly (vinylpyrroldone) (PVP) Polymer Blend Electrolyte
Authors: Alfannakh Huda 
Published: February 2021

Abstract
Blend sample based on Poly (vinyl alcohol) (PVA)/Poly (vinylpyrroldone) (PVP), (50:50), and their blend electrolyte with different weigh ratio of Iron (II) Chloride (FeCl2), (0.5, 1 and 2 wt.%), have been prepared using casting method. The AC electrical properties of these samples were investigated in the frequency range 1 kHz to 1 MHz and in temperature ranging from 30 to 150 °C. The real and imaginary impedance, frequency and temperature dependence, indicate the enhancement of the electrical conductivity of the blend due to addition of FeCl2. Pure and electrolyte blend samples were found to be characterized by a plateau region at low frequency and high temperature, and this plateau region increases with increase in temperature and /or FeCl2 weight ratio. Nyquist plots for pure and blend electrolyte samples presented depressed semicircles at all temperatures. For pure blend sample, the ac conductivity was attributed to hopping mechanism while for blend electrolyte sample ionic conduction represents the predominant conduction mechanism. Jonscher universal power law was used to study the conduction mechanism for pure and electrolyte blend sample. The activation energy for blend and blend electrolyte samples was calculated and two activation regions were appeared for blend electrolyte samples.
Tapping Into the Aging Brain: in Vivo Microdialysis Reveals Mirroring Pathology Between Preclinical Models and Patients With Alzheimer’s Disease
Authors: Bjorkli Christiana , Louet Claire , Flo Trude Helen , Hemler Mary , Sandvig Axel , Sandvig Ioanna 
Published: February 2021

Abstract
BackgroundPreclinical models of Alzheimer’s disease (AD) can provide valuable insights into the onset and progression of the disease, such as changes in concentrations of amyloid-β (Aβ) and tau in cerebrospinal fluid (CSF). However, such models are currently underutilized due to limited advancement in techniques that allow for longitudinal monitoring of CSF akin to methods employed in AD patients. An elegant way to understand the biochemical environment in the diseased brain is intracerebral microdialysis, a method that has until now been limited to short-term observations, or snapshots, of the brain microenvironment. ResultsOur novel push-pull microdialysis method in AD mice permits in vivo longitudinal monitoring of dynamic changes of Aβ and tau in CSF and allows for better translational understanding of CSF biomarkers. Specifically, we demonstrate that CSF concentrations of Aβ and tau along disease progression in transgenic mice mirror what is observed in patients, with a decrease in CSF Aβ observed when plaques are deposited, and an increase in CSF tau once tau pathology is present in the brain. We found that a high molecular weight cut-off membrane allowed for simultaneous sampling of Aβ and tau, comparable to lumbar puncture CSF collection in patients. We furthermore provide specific recommendations for optimal application of our novel microdialysis method, such as achieving optimal recovery of analytes, which depends heavily on the flow rate of perfusion, probe properties and perfusate composition. ConclusionsOur approach can further advance AD research by following evolving neuropathology along the disease cascade via consecutive sampling from the same animal, and can additionally be used to administer pharmaceutical compounds and assess their efficacy in treating AD.
Determination of key-thermodynamic parameters using a kinetic modeling approach to describe the post-consumer poly(ethylene terephthalate) hydrolysis catalyzed by cutinase from Humicola insolens
Authors: Eugenio Erika de Queiros , Campisano Ivone Sampaio Pereira , Castro Aline Machado de , Coelho Maria Alice Zarur , Langone Marta Antunes Pereira 
Published: February 2021

Abstract
The search for a straightforward technology for post-consumer poly(ethylene terephthalate) (PC-PET) degradation is essential to develop a circular economy. In this context, PET hydrolases such as cutinases can be used as bioplatforms for this purpose. Humicola insolens cutinase (HiC) is a promising biocatalyst for PC-PET hydrolysis. Therefore, this work evaluated a kinetic model, and it was observed that the HiC seems not to be inhibited by any of the main PET hydrolysis products such as terephthalic acid (TPA), mono-(2-hydroxyethyl) terephthalate (MHET), and bis-(2-hydroxyethyl) terephthalate (BHET). The excellent fitting of the experimental data to a kinetic model based on enzyme-limiting conditions validates its employment for describing the enzymatic PC-PET hydrolysis using two-particle size ranges (0.075-0.250, and 0.250-0.600 mm) and temperatures (40, 50, 55, 60, 70, and 80 ºC). The Arrhenius law provided a reliable parameter (activation energy of 98.9 ± 2.6 kJ mol −1 ) for enzymatic hydrolysis, which compares well with reported values for chemical PET hydrolysis. The thermodynamic parameters of PC-PET hydrolysis corresponded to activation enthalpy of 96.1 ± 3.6 kJ mol -1 and activation entropy of 10.8 ± 9.8 J mol -1 K -1 . Thus, the observed rate enhancement with temperature was attributed to the enthalpic contribution, and this understanding is helpful to the comprehension of enzymatic behavior on hydrolysis reaction.
Co-morbidity of attention deficit hyper activity disorder and associated factors among children with seizure disorder attending pediatric seizure clinic a cross-sectional study at Gondar University hospital (2016), northwest, Ethiopia
Authors: Sebhat Haregewoin Mulat , Muluneh Niguse , Eyasu Tewodros 
Published: February 2021

Abstract
BackgroundWhen attention deficit Hyperactivity Disorder (ADHD) presents in epileptic children, it makes the treatment complicated and the prognosis poor. Hence, understanding the magnitude of ADHD and associated factors would be important to have a policy intention towards these people and to design appropriate interventions. Therefore, the current study was conducted to determine co-morbidity of attention deficit hyperactivity disorder (ADHD) and associated factors in children with seizure disorders.Methods A hospital based cross-sectional study was conducted by taking 260children who had follow ups in the pediatric seizure clinic. The systematic random sampling technique was used to recruit participants. A structured, pretested and interviewer administered questionnaire which included questions on associated factors and standard disruptive behavioural disorder (DBD) rating scale was used to collect data. Data were coded, entered and cleaned by using the Epi-Data version 3.1 and exported to SPSS version 20 for further analysis. The multivariate binary logistic regression was used to cheek the association between independent and dependent variables. Variables with significant associations were identified on the basis of OR, with a 95%CI and p-value of < 0.05 and considered as statistically significant.ResultThe prevalence of ADHD among epileptic children was found to be 115(44.2%),with a confidence interval of (38.1-50.5),out of which only 3(2.6%) were detected as having mental health problems by the clinician. The predominant subtype was inattentive type 96(61.1%). Factors significantly associated with ADHD were male sex (AOR = 2.70 CI: 1.46-4.97), family history of seizure disorder (AOR=2.42CI:1.26-4.65), family history mental illnesses (AOR=4.14 CI: 1.76-9.68), sudden onset of seizure (AOR=2.37 CI: 1.32-4.27) and uncontrolled seizure (AOR=2.55 CI: 1.41-4.61). Conclusion Attention deficit hyperactivity disorder was common among children with seizure disorders in the study area. Therefore, interventions that would address such factors would help to overcome further complications.
Moderately Hypofractionated Intensity Modulated Radiotherapy (IMRT) With a Simultaneous Integrated Boost for Locally Advanced Head and Cancer – Do Modern Techniques Held Their Promise?
Authors: Wichmann Jörn , Durisin Martin , Hermann Robert Michael , Merten Roland , Christiansen Hans 
Published: February 2021

Abstract
PurposeIntensity-modulated-radiotherapy (IMRT) is still a standard of care for radiotherapy in locally advanced head and neck cancer (LA-HNSCC). Simultaneous-integrated-boost (SIB) and moderately hypofractionation offer an opportunity of individual dose painting and reduction of overall treatment time. We present retrospective data on toxicity and local-regional-control of a patient cohort with LA-HNSCC treated with an IMRT-SIB-concept in comparison to normofractionated 3D-conformal radiotherapy (3D-RT) after a long-term follow-up.MethodsBetween 2012 and 2014, n=67 patients with HNSCC (stages III/IV without distant metastases) were treated with IMRT-SIB either definitive (single/total doses: 2.2/66Gy, 2.08/62.4Gy, 1.8/54Gy in 30 fractions) or in the postoperative setting (2.08/62.4Gy, 1.92/57.6Gy, 1.8/54Gy). These patients' clinical course was matched (for gender, primary, and treatment concept) as part of a matched-pair-analysis with patients treated before mid-2012 with normofractionated 3D-CRT (definitive: 2Gy/50Gy followed by a sequential boost up to 70Gy; postoperative: 2Gy/60-64Gy). Chemotherapy/immunotherapy was given concomitantly in both groups in the definitive situation (postoperative dependent on risk factors). Primary endpoints were acute and late toxicity; secondary endpoint was loco-regional-control (LRC).Results67 patients treated with IMRT-SIB (n = 20 definitive, n = 47 adjuvant) were matched with 67 patients treated with 3D-RT. There were minor imbalances between the groups concerning non-matching-variables like extracapsular extension (ECE) and chemotherapy in IMRT-SIB.Significantly less toxicity was found in favor of IMRT-SIB concerning dysphagia, radiation dermatitis, xerostomia, fibrosis, and lymphoedema. After a median follow-up of 63 months, median LRC was not reached (IMRT-SIB) vs. 69.5m (3D-RT) (p=0.63).ConclusionThis moderately hypofractionated IMRT-SIB-concept showed to be feasible with less toxicity compared to conventional 3D-RT in this long-term follow-up observation.
Electron Irradiation Eﬀects in Au thin Flms
Authors: Oliveira Franciele Silva Mendes de , Nogueira Maurício Jesuíno , Fabrim Zacarias Eduardo , Fichtner Paulo F P 
Published: February 2021

Abstract
Room temperature 200 keV electron irradiation eﬀects on the area retraction behavior presented by 6.75 nm thick Au thin ﬁlms deposited over a self-standing SiO 2 / silicon nitride membrane are investigated as a function of the irradiation ﬂuence Φ. The as-deposited ﬁlms already contain void discontinuities. The void growth behavior is investigated considering irradiation and thermally-induced surface atoms’ migration. The ﬁlm’s coverage area A(Φ) and void perimeter P(Φ), obtained via Transmission Electron Microscopy observations, allow for calculating the atomic displacement causing the area retraction. This data is compared with model calculations of irradiation and thermally-induced atomic ﬂuxes. The results demonstrate that the balance between the thermal and irradiation processes strongly depends on the choice of the surface thermal diﬀusivity values, which present large discrepancies in the literature. Our results suggest that irradiation-induced atomic displacements follow the same thermodynamic driving forces acting in thermal processes. The work also discloses a new method to investigate surface atoms’ behavior and promote microstructural modiﬁcations at room or lower temperatures.
Functionally Confirmed Compound Heterozygous ADAM17 Missense Loss-of-function Variants Cause Neonatal Inflammatory Skin and Bowel Disease 1
Authors: Imoto Issei , Saito Masako , Suga Kenichi , Kohmoto Tomohiro , Otsu Masanobu , Horiuchi Keisuke , Nakayama Hironao , Higashiyama Shigeki , Sugimoto Mayumi , Sasaki Ayumi , Homma Yukako , Shono Miki , Nakagawa Ryuji , Hayabuchi Yasunobu , Tange Shoichiro , Kagami Shoji , Masuda Kiyoshi 
Published: February 2021

Abstract
A disintegrin and metalloprotease 17 (ADAM17) is the major sheddase that processes more than 80 substrates, including tumour necrosis factor-α (TNFα). The homozygous genetic deficiency of ADAM17 causing a complete loss of ADAM17 expression was reported to be linked to neonatal inflammatory skin and bowel disease 1 (NISBD1). Here we report for the first time, a family with NISBD1 caused by functionally confirmed compound heterozygous missense variants of ADAM17, namely c.1699T > C (p.Cys567Arg) and c.1799G > A (p.Cys600Tyr). Both variants were detected in two siblings with clinical features of NISBD1, such as erythroderma with exudate in whole body, recurrent skin infection and sepsis and prolonged diarrhoea. In a cell-based assay using Adam10/17 double-knockout mouse embryonic fibroblasts (Adam10/17−/− mEFs) exogenously expressing each of these mutants, phorbol 12-myristate 13-acetate (PMA)-stimulated shedding was strongly reduced compared with wild-type ADAM17. Moreover, lysates from Adam10/17−/− mEFs exogenously expressing each mutant exhibited impaired basal and PMA-stimulated TNFα converting enzyme activity. Thus, in vitro functional assays demonstrated that both missense variants cause the complete loss-of-function of ADAM17, resulting in the development of NISBD1. Our study further expands the spectrum of genetic pathology underlying ADAM17 in NISBD1 and establishes functional assay systems for its missense variants.
The Effect of MAOA Gene × Gender on the Relationship between Negative Network News Browsing Preference and Aggression among College Students
Authors: Gao Fengqiang , Guo Zongxin , Zhan Xiangping , Wang Jun , Shi Huimin , Yan Yun , Sun Yu , Wang Peng 
Published: February 2021

Abstract
The present research aims to examine whether and how the negative network news browsing preference (NNNBP) affect individual’s aggression. Two studies were conducted in the current research: study 1 developed a new measurement scale—network news browsing preference questionnaire(NNBPQ). The results indicated it had appropriate reliability (Cronbach’s α = 0.93) and validity (χ 2 /df = 1.92, CFI = 0.94, NFI = 0.89, GFI = 0.89，TLI = 0.94，RMSEA = 0.05) , which was conformed to psychometrics standards, and could be used for further study. Study 2 explored the relationship between NNNBP and aggression, and the moderate effect of MAOA gene × gender among 352 college students. The results indicated that: (a) NNNBP could positively predict male college students’ hostility and total score. It also could positively predict female college students’ physical aggression, verbal aggression, hostility and total score. (b) MAOA Gene × gender had moderate effect on the relationship between NNNBP and aggression; more specially, NNNBP could positively predict female G allele carriers’ physical aggression and hostility, while didn’t show significant under other conditions. Finally, the limitations and future prospects of the present research were discussed.
The Rescue and the Selection of the Thermally Stabled Type O Vaccine Candidate Strains of Foot-and-mouth Disease Virus
Authors: Li Dong , Gao Ya , Li Pinghua , Ma Xueqing , Bai Xingwen , Sun Pu , Du Ping , Yuan Hong , Cao Yimei , Li Kun , Fu Yuanfang , Zhang Jing , Bao Huifang , Chen Yingli , Li Zhiyong , Lu Zengjun , Liu Zaixin 
Published: February 2021

Abstract
The inactivated vaccines of Foot-and-mouth disease virus (FMDV) have been used widely in the world to control Foot-and-mouth disease (FMD). But the virions (146S) of this virus are easily dissociated into pentamer subunits (12S), thus limits the immune protective efficacy of the inactivated vaccines when the temperature is higher than 30°C. The cold-chain system can maintain the quality of the vaccines, but that is usually not reliable in limited resource settings. Thus, it is imperative to improve the thermostability of vaccine strains to guarantee the vaccines’ quality. In this study, 4 recombinant FMDV strains containing single or multiple amino acids substitutions in the structural proteins (SP) were rescued by using a pre-constructed FMDV type O full-length infectious clone (pO/DY-VP1). The assays used here indicated that the single or multiple amino acids substitutions in SP may affect the viral replications to different degrees. Furthermore, the heat and acid stabilities of the recombinant viruses were significantly improved comparing with the parental virus. Three well thermally stable strains of recombinant viruses (rHN/DY-VP1Y2098F, rHN/DY-VP1V2090A-S2093H and rHN/DY-VP1V2090A-S2093H-Y2098F) were selected for inactivated vaccine to immunize pigs. Blood samples were collected every week to prepare sera. Meanwhile the effects of mutations in SP amino acids on the antigenicity were analyzed by viral neutralization test, which showed that the substitutions of S2093H, Y2098F and S2093H-Y2098F did not affect the immunogenicity. In addition, comparing to the parental virus, Y2098F mutation could increase the thermostability significantly (p<0.05). In conclusion, the rHN/DY-VP1Y2098F mutant is considered as a potential vaccine strain in the future.
Assessing the Usability of Complex Psychosocial Interventions: The Intervention Usability Scale
Authors: Lyon Aaron R , Pullmann Michael D. , Jacobson Jedediah , Osterhage Katie , Achkar Morhaf Al , Renn Brenna N. , Munson Sean A. , Areán Patricia A. 
Published: February 2021

Abstract
Background. Usability – the extent to which an intervention can be used by specified users to achieve specified goals with effectiveness, efficiency, and satisfaction – is a key determinant of implementation success. However, few instruments have been developed to measure the design quality of complex health interventions (i.e., those with several interacting components). This study evaluated the structural validity of the Intervention Usability Scale (IUS), an adapted version of the well-established System Usability Scale (SUS) for digital technologies, to measure the usability of a leading complex psychosocial intervention, Motivational Interviewing (MI), for behavioral health service delivery in primary care. Prior SUS studies have found both one- and two-factor solutions, both of which were examined in the current study of the IUS.Method. A survey administered to 136 medical professionals from 11 primary care sites collected demographic information and IUS ratings for MI, the evidence-based psychosocial intervention that primary care providers reported using most often for behavioral health service delivery. Factor analyses replicated procedures used in prior research on the SUS.Results. Analyses indicated that a two-factor solution (with “usable” and “learnable” subscales) best fit the data, accounting for 54.1% of the variance. Inter-item reliabilities for the total score, usable subscale, and learnable subscale were α = .83, α = .84, and α = .67, respectively.Conclusions. This study provides evidence for a two-factor IUS structure consistent with some prior research, as well as acceptable reliability. Implications for implementation research evaluating the usability of complex health interventions are discussed, including the potential for future comparisons across multiple interventions and provider types, as well as the use of the IUS to evaluate the relationship between usability and implementation outcomes such as feasibility.