bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex evaluation of calendula officinalis flowers by main biologically active substances content
Authors: Malankina E. L. , Kozlovskaya L. N. , Biktimyrova L. V. , Komarova E. L. 
Published: March 2021

Relevance. The demand for medicinal raw materials of Calendula officinalis L. is determined by the high content of pharmacologically significant compounds. Comparison of the most common ornamental cultivars of Calendula officinalis L.) in terms of yield and content of biologically active substances in the raw material helps to identify the most promising cultivars in terms of a set of indicators.Materials and methods.The decorative cultivars Apricot, Art Shads, Fiesta Gitana, Kabluna golden-yellow, Orange King, Orange Balls, Yellow Gitana and Golden Balls, characterized by large inflorescences and terry, were used as prototypes. The cultivar Kalta, widely used for the production of medicinal raw materials, was chosen as a control. The experiments were laid on the experimental plots of the Fruit Growing Laboratory of the Russian State Agricultural University – Moscow Timiryazev Agricultural Academy. Productivity was determined as the total weight of inflorescences harvested during the entire season per unit area. The amount of flavonoids was determined by spectrophotometry. The amount of polysaccharides was determined according to the modified Draywood method. The lutein content was determined by HPLC.Results.To obtain inflorescences we can recommend to use the variety Orange King, which, with a satisfactory yield (0.363 kg/m2), was characterized by a high content of flavonoids (1.2%) and satisfactory (35.9%) extractives and corresponds to the quality of raw materials required by GF XIV. In addition, this variety was characterized by an increased content of polysaccharides (20.3%). The Varieties Apricot and Yellow Gitana were characterized by high productivity (562±49 and 507±41 g/m2, respectively). Their raw materials can be used by production of herbal teas, where the content of flavonoids is not regulated. As sources of lutein, one should pay attention to the ligulate flowers of the varieties Apricot, Fiesta Gitana, Golden balls. In the variety Kabluna, with the maximum lutein content, the proportion of reed flowers in the inflorescence was only 38%. In varieties with large inflorescences, the content of lutein in reed flowers was higher (R=0.685). Large non-double inflorescences will be most productive in this indicator.
Description of Lujinxingia Vulgaris Sp. Nov., Isolated From Coastal Sediment via Prey-traps
Authors: Wang Shuo , Mu Da-Shuai , Li Guang-Yu , Du Zong-Jun 
Published: March 2021

Abstract
Two Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacilli, designated strain TMQ4T and TMQ2, were isolated from Xiaoshi Island in Weihai, PR China. The two strains had the abilities of predating other bacteria and were lured out with the prey Acinetobacter baumannii ATCC 19606T. Growth was observed within the ranges 25–45 oC (optimally at 37 oC), pH 6.5–9.0 (optimally at pH 7.5–8.0) and 1–8 % NaCl (optimally at 3–4 %, w/v). The draft genome sequences of strains TMQ4T and TMQ2 contained 184 contigs of 5,609,735 bp with a G+C content of 64.4 mol% and 148 contigs of 5,589,985 bp with a G+C content of 65.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed two strains belonged to the genus Lujinxingia, and their closest neighbour was Lujinxingia sediminis SEH01T, both with the similarity of 98.9 %. The phylogenomic topologies and analyses also demonstrated the taxonomic status of the two strains. Based on genomic analyses, the two strains lost biosynthesis pathway of several kinds of important chemical compounds and possessed the predatory indices of 2. Iso-C15:0 was contained in the predominant cellular fatty acids for strains TMQ4T and TMQ2. The major polar lipids of both strains consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids, and the respiratory quinone was menaquinone MK-7 for both strains. The physiological, biochemical and phylogenetic properties suggest that two strains represent a novel species within the genus Lujinxingia. The name Lujinxingia vulgaris sp. nov. is proposed, with strain TMQ4T (= KCTC 62851T = MCCC 1H00392T) as type strain and strain TMQ2 (=KCTC 72079 = MCCC 1H00381) as reference strain.
Cardiac Events in Kawasaki Disease With Coronary Aneurysms Revisited, A Two-center Retrospective Cohort Study in Thailand
Authors: Santimahakullert Kanokvalee , Vijarnsorn Chodchanok , Wongswadiwat Yuttapong , Chanthong Prakul , Khrongsrattha Sappaya , Panamonta Manat , Chan-on Paradorn , Durongpisitkul Kritvikrom , Chungsomprasong Paweena , Kanjanauthai Supaluck , Soongswang Jarupim 
Published: March 2021

Abstract
Kawasaki disease (KD) is a common vasculitis in children, which may be complicated with coronary artery aneurysms (CAAs). We aimed to report the rates of major adverse cardiac events (MACE) and determine the risks of MACE in children diagnosed with KD and CAAs in Thailand. Data of 170 children diagnosed with KD and CAAs in two centers of Thailand between 1994 and 2019 was retrospectively reviewed. The risks of MACE were analyzed using multivariate analysis. Of 170 patients, forty-nine patients (28.8%) had giant CAAs. During the median time of follow-up (5.4 years; ranging from 22 days to 23 years), 19 patients (11.1%) experienced MACE including 12 coronary artery bypass grafting, 2 percutaneous coronary intervention and 5 patients with evidence of coronary occlusion. Coronary interventions were performed at 4 years (ranging from 0.01 to 9.5 years) after the KD diagnosis. Independent risks of MACE in KD with CAAs were from the presence of giant aneurysms (HR 16.55; 95% CI 2.52 to 108.63; p=0.003) and lack of intravenous immunoglobulin (IVIG) treatment (HR 11.43; 95% CI 2.8 to 46.62; p=0.001). The intervention-free rate at 5 and 10 years in patients with giant CAAs was 78.7% and 52.2%, respectively.Trial registration: TCTR20190125004
Production of Indole-3-Acetic Acid: A White Biotechnology for Weed Biocontrol
Authors: Bunsangiam Sakaoduoen , Thongpae Nutnaree , Limtong Savitree , Sri Nantana 
Published: March 2021

Abstract
Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade L-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3,569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.
Echocardiographic and Clinical Features in Diagnosis of Rare Cardiac Myxomas
Authors: yanhua qi , Ma miaoyan , Wei Yajuan , wu jinfang , gao peirong , li xiaopeng , liu baomin 
Published: March 2021

Abstract
Background: Cardiac myxoma(CM) is a rare cardiac disease, with the majority located in the left atrium and less commonly in the right atrium, right ventricular, biatrial, left ventricular, right ventricular outflow tract or heart valves. These rare CMs need to be distinguished from other masses. The purses of our study is to provide important value in finding the extra-atrial tumor and making the correct diagnosis by reviewed echocardiographic and clinical features of five rare CMs. Methods: We reviewed medical records and echocardiographic data in all patients with surgery diagnosis of rare CMs from January 2019 to June 2020 in the second affiliated hospital, Xi’an Jiaotong University. Results: Five patients were enrolled in our study. The sites of CMs were rare and located separately on RV, PV, LV, LA and LVOT, LA. Four cases was myxomas and one was lipoma. Five patients recovered well after operation. Conclusions: Cardiac tumors developed in unusual locations should be considered to exclude the possibility of myxoma. ﻿Subheadings: Echocardiographic of rare Cardiac Myxomas
Validity of the ACTS Intimate Partner Violence Screen in Antenatal Care: A Cross Sectional Study
Authors: Hegarty K , Spangaro J , Kyei-Onanjiri M , Valpied J , Walsh J , Chapman J , Koziol-McLain J 
Published: March 2021

Abstract
Background: Intimate partner violence (IPV) is a major public health problem with harmful consequences. In Australia, there is no national standard screening tool and screening practice is variable across states. The objectives of this study were to assess in the antenatal healthcare setting: i) the validity of a new IPV brief screening tool and ii) women’s preference for screening response format, screening frequency and comfort level.Methods: 1,067 antenatal patients in a major metropolitan Victorian hospital in Australia completed a paper-based, self-administered survey. The survey included four screening items about whether they were Afraid/Controlled/Threatened/ Slapped or physically hurt (ACTS) by a partner or ex-partner in the last 12 months; and the Composite Abuse Scale (reference standard). The ACTS screen was presented firstly with a binary yes/no response format and then with a five-point ordinal frequency format from ‘never’ (0) to ‘very frequently’ (4). The main outcome measures were test statistics of the four-item ACTS screening tool (sensitivity, specificity, predictive values, and area under the curve) against the reference standard and women’s screening preferences.Results: Twelve-month IPV prevalence varied depending on the ACTS response format with 8% (83) positive on ACTS yes/no format, 12.8% (133) positive on ACTS ordinal frequency format and 10.5% (108) on the reference Composite Abuse Scale. Overall, the ACTS screening tool demonstrated clinical utility for the ordinal frequency format (AUC, 0.80; 95% CI = 0.76 to 0.85) and the binary yes/no format (AUC, 0.74, 95% CI = 0.69 to 0.79). The frequency scale (66%) had greater sensitivity than the yes/no scale (51%). The positive and negative predictive values were 56% and 96% for the frequency scale and 68% and 95% for the yes/no scale. Specificity was high regardless of screening question response options. Half (53%) of the women categorised as abused preferred the yes/no scale. Around half of the women (48%, 472) thought health care providers should ask pregnant women about IPV at every visit.Conclusions: The four-item ACTS tool (using the frequency scale and a cut-off of one on any item) is recommended for written self-administered screening of women to identify those experiencing IPV to enable first-line response and follow-up.
Interactive Effects of Sediments and Urchins on Tropical Macroalgal Forests
Authors: Harris Rosalie , Wilson Shaun , Fulton Christopher 
Published: March 2021

Abstract
Macroalgal forests are a prominent component of tropical seascapes providing food and shelter for many species whilst subsidising secondary productivity in coastal ecosystems. Monitoring and managing macroalgae requires an understanding of key processes that alter these assemblages, especially at the local scale. Here we consider how sediment depth/cover and abundance of the macroalgal grazing urchin Tripneustes gratilla (Linnaeus, 1758), influence variation in community composition and physical structure of macroalgal patches within the World Heritage listed Ningaloo Reef, Western Australia. Our study found high urchin densities in sites with lower sediment loads and that sediments, urchin abundance and seascape position combined, explained 32% of the spatial variation in macroalgal forest community composition. We also found a greater occurrence of Sargassopsis, Caulerpa, Sirophysalis and Hormophysa in the lagoon where sediment cover/depth was high. Canopy height and cover of canopy forming macroalgae was also greater where sediment loads were high and urchin abundance low. Macroalgal genera with a strong and robust thallus were found in sediment depths up to 6 cm as opposed to those with softer, fragile thalli that were found in sediments < 2 cm. Our results demonstrate that high densities of urchins and mobile sediments can have profound effects on the composition and structure of tropical macroalgal forests. Changes to macroalgal forest community composition and physical structure will have flow-on effects for ecological processes such as productivity and habitat use which can alter the ecosystem goods and services macroalgal forests provide.
A Randomized Controlled Trial of Intranasal Oxytocin in Phelan-McDermid Syndrome
Authors: Fastman Jarrett , Foss-Feig Jennifer , Frank Yitzchak , Halpern Danielle , Harony-Nicolas Hala , Layton Christina , Sandin Sven , Siper Paige , Tang Lara , Trelles Pilar , Zweifach Jessica , Buxbaum Joseph D. , Kolevzon Alexander 
Published: March 2021

Abstract
BackgroundPhelan-McDermid syndrome (PMS) is a rare neurodevelopmental disorder caused by haploinsufficiency of the SHANK3 gene and characterized by global developmental delays, deficits in speech and motor function, and autism spectrum disorder (ASD). Monogenic causes of ASD such as PMS are well suited to investigations with novel therapeutics, as interventions can be targeted based on established genetic etiology. While preclinical studies have demonstrated that the neuropeptide oxytocin can reverse electrophysiological, attentional, and social recognition memory deficits in Shank3-deficient rats, there have been no trials in individuals with PMS. The purpose of this study is to assess the efficacy and safety of intranasal oxytocin as a treatment for the core symptoms of ASD in a cohort of children with PMS.MethodsEighteen children aged 5-17 with PMS were enrolled. Participants were randomized to receive intranasal oxytocin or placebo (intranasal saline) and underwent treatment during a 12-week double-blind, parallel group phase followed by a 12-week open-label extension phase during which all participants received intranasal oxytocin. Safety was monitored throughout the study period and efficacy was assessed using the primary outcome of the Aberrant Behavior Checklist – Social Withdrawal (ABC-SW) subscale as well as a number of secondary outcome measures related to the core symptoms of ASD.ResultsThere was no statistically significant improvement with oxytocin as compared to placebo on the ABC-SW (Mann-Whitney U=50, p=0.055), or on any secondary outcome measures, during either the double-blind or open-label phases. Oxytocin was generally well tolerated and there were no serious adverse events.Limitations: The small sample size, potential challenges with drug administration, and expectancy bias due to relying on parent reported outcome measures may all contribute to limitations in interpreting results.ConclusionOur results suggest that intranasal oxytocin is not efficacious in improving the core symptoms of ASD in children with PMS.Trial registration: NCT02710084
Wildfire Severity Zoning Through Google Earth Engine and Fire Risk Assessment: Application of Data Mining and Fuzzy Multi-Criteria Evaluation in Zagros Forests, Iran
Authors: Heidari Hamed , Keshtkar Mostafa , Moazzeni Niloofar , Jafari Meisam , Azadi Hossein 
Published: March 2021

Abstract
The arid and semi-arid regions of Zagros forests in the Middle East are constantly exposed to wildfire due to ecological conditions, and support systems are inefficient in controlling wildfires due to managerial and social weaknesses. Remote sensing and assessment tools are suitable for rapid prevention and action to identify the severity and location of a wildfire. This study investigated the natural resource management of Zagros Forestry in terms of protecting wildfire and combating forest wildfires using the NASA fire spatial data and the wildfire severity in the Google Earth Engine (GEE) platform. The land-use of the study area is produced by applying the Random Forest (RF) classification method and data from the Sentinel 2 satellite imagery for 2019. To separate the types of cultivation and vegetation of the region, the method of extracting the average vegetation index of the seasons is extracted from GEE. To evaluate fire risk, eleven human and ecological factors and two assessment models are applied to classify the probability fire risk therein. Furthermore, the outcome of AUC confirmed the Logistic Regression (LR) model; the accuracy of the LR (AUC=0.875049) model is satisfactory and is suitable for fire risk mapping in Zagros Forestry. Six high-risk areas of the wildfire were identified by MOLA, which overlap with protected areas. Out of a total of 20469.17 Ha of wildfire, 10426.41 Ha belong to these protected areas. 3826 Ha of this area were in the forests of Amygdalus spp, Quercus brant ii, pistacia Atlantica, and Quercus Infectoria, and 6600.41 Ha of it were in rangelands. Accordingly, an executive order was developed for the decision support system that reduces the risk of wildfire and helps extinguish the wildfire.
Causal Path of COPD Progression-Associated Genes in Different Biological Samples
Authors: Mostafaei Shayan , Borna Hojat , Emamvirdizadeh Alireza , Arabfard Masoud , Ahmadi Ali , Salimian Jafar , Salesi Mahmoud , jamalkandi Sadegh Azimzadeh 
Published: March 2021

Abstract
Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory disease with pulmonary and extra-pulmonary complications. Due to the disease's systemic nature, many investigations investigated the genetic alterations in various biological samples. We aimed to infer causal genes in COPD's pathogenesis in different biological samples using elastic-net logistic regression and the Structural Equation Model. Samples of small airway epithelial cells, bronchoalveolar lavage macrophages, lung tissue biopsy, sputum, and blood samples were selected (135, 70, 235, 143, and 226 samples, respectively). Elastic-net Logistic Regression analysis was implemented to identify the most important genes involved in COPD progression. Thirty-three candidate genes were identified as essential factors in the pathogenesis of COPD and regulation of lung function. Recognized candidate genes in SAE cells have the highest area under the ROC curve (AUC=97%, SD= 3.9%). Our analysis indicates that macrophages and epithelial cells are more influential in COPD progression at the transcriptome level.
Abiraterone versus bicalutamide in combination with gonadotropin releasing hormone antagonist therapy for high risk metastatic hormone sensitive prostate cancer.
Authors: Ueda Takashi , Shiraishi Takumi , Ueda Saya , Ohashi Motoharu , Matsugasumi Toru , Yamada Yasuhiro , Fujihara Atsuko , Hongo Fumiya , Okihara Koji , Ukimura Osamu 
Published: March 2021

Abstract
ObjectivesTo compare the efficacy of abiraterone with that of bicalutamide in combination with gonadotropin-releasing hormone antagonist treatment for high risk metastatic hormone-sensitive prostate cancer patients.MethodsOne hundred and forty-nine patients with high risk metastatic hormone-sensitive prostate cancer at our hospital and affiliated hospitals between December 2013 and July 2020 were retrospectively identified. Fifty patients were administered abiraterone (1000mg/day) plus prednisolone (5mg/day) with gonadotropin-releasing hormone antagonist (degarelix) (group A) and 99 patients were administered bicalutamide (80mg/day) with gonadotropin-releasing hormone antagonist (group B). ResultsPSA- progression-free survival of group A was significantly longer than that of group B. Abiraterone therapy and Gleason score were significant independent factors for PSA-progression-free survival. By propensity score matching, total 56 matched patients were obtained. PSA-PFS (p<0.001) and OS (p=0.0071) of high risk mHNPC patients were significantly longer in abiraterone group of matched patients. Abiraterone therapy and Gleason score were still shown to be significant independent factors for PSA-PFS in matched patients.ConclusionsPSA-progression-free survival and overall survival in patients who were treated with abiraterone in combination with gonadotropin releasing hormone antagonist were significantly better than those of bicalutamide.
Virtual screening, molecular dynamics and binding energy-MM-PBSA studies of natural compounds to identify potential EcR inhibitors against Bemisia tabaci Gennadius
Authors: Mangat Harmilan Kaur , Rani Manisha , Pathak Rajesh Kumar , Yadav Inderjit Singh , Utreja Divya , Chhuneja Pardeep Kumar , Chhuneja Parveen 
Published: March 2021

Abstract
Whitefly (Bemisia tabaci Gennadius) is a hmpteran phyto polyphagous sucking insect pest which is an important pest of cotton that causes economic losses to the crop by reducing its yield and quality. Ecdysteroids like 20-hydroxy ecdysone (20-E), have significant role in larval moulting, development, and reproduction in pterygota insects. Intending to obstruct these fundamental, developmental physiological processes, the receptor of 20-E, the Ecdysone Receptor (BtEcR) of Bemisia tabaci has been targeted. To identify potent inhibitors of BtEcr, 98,072 natural compounds were retrieved from ZINC database. A structure-based virtual screening of these compounds was conducted for their binding to BtEcR and the top two compounds (ZINC08952607 and ZINC04264850) were selected based on minimum binding energy. 50 ns molecular dynamics simulation (MDS) study was then performed for the prediction of dynamics and stability of BtEcR and top-scoring ligand-BtEcR complexes. Besides, g_mmpbsa tool was used to calculate and analyse binding free energy of BtEcR-ligand complexes. The results revealed that ZINC08952607 and ZINC04264850 had binding free energy of −170.156 kJ/mol and −200.349 kJ/mol, respectively. Thus, these ligands can be utilized as lead compounds for the development of environmentally safe insecticides against the whitefly.
The Excitatory/Inhibitory Balance of Synaptic Transmission Is Impaired at Higher Extracellular Ca2+ Concentrations in Scn1a+/− Mouse Model of Dravet Syndrome
Authors: Uchino Kouya , Kawano Hiroyuki , Tanaka Yasuyoshi , Adaniya Yuna , Asahara Ai , Deshimaru Masanobu , Kubota Kaori , Watanabe Takuya , Katsurabayashi Shutaro , Iwasaki Katsunori , Hirose Shinichi 
Published: March 2021

Abstract
Dravet syndrome (DS) is an intractable form of childhood epilepsy that occurs in infancy. More than 80% of all patients have a heterozygous abnormality in the SCN1A gene, which encodes a subunit of Na+ channels in the brain. However, the detailed pathogenesis of DS remains unclear. This study investigated the synaptic pathogenesis of this disease in terms of excitatory/inhibitory balance using a mouse model of DS. We show that excitatory postsynaptic currents were similar between Scn1a knock-in neurons (Scn1a+/− neurons) and wild-type neurons, but inhibitory postsynaptic currents were significantly lower in Scn1a+/− neurons. Moreover, both the vesicular release probability and the number of inhibitory synapses were significantly lower in Scn1a+/− neurons compared with wild-type neurons. There was no proportional increase in inhibitory postsynaptic current amplitude in response to increased extracellular Ca2+ concentrations. Our study revealed that the number of inhibitory synapses is significantly reduced in Scn1a+/− neurons, while the sensitivity of inhibitory synapses to extracellular Ca2+ concentrations is markedly increased. These data suggest that Ca2+ tethering in inhibitory nerve terminals may be disturbed following the synaptic burst, likely leading to epileptic symptoms.