bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark
Authors: Anokwah Daniel , Asante-Kwatia Evelyn , Mensah Abraham Y. , Danquah Cynthia Amaning , Harley Benjamin K. , Amponsah Isaac Kingsley , Oberer Lukas 
Published: March 2021

AbstractBackgroundAntimicrobial resistance is a global health challenge. The involvement of bacterial biofilms and efflux pumps in the development of multidrug resistance (MDR) is well established. Medicinal plants have been proposed as alternatives for combating MDR focusing on their bioactive constituents with resistance modulatory activities. This study was aimed at investigating the stem bark ofAidia genipiflorafor bioactive constituents with anti-biofilm, efflux pump inhibition and resistance modulatory activities.MethodThe crude methanol extract was purified by column chromatography and isolated compounds characterized by mass and nuclear magnetic resonance spectrometry. Antibacterial activity was determined by the High-throughput spot culture growth inhibition and the broth micro-dilution assay. The ethidium bromide accumulation assay was used to determine efflux pump inhibition property. Biofilm inhibition was determined in a microplate crystal violet retention assay.ResultsPurification of the ethyl acetate fraction led to the isolation of oleanonic acid (1), 4-hydroxy cinnamic acid docosyl ester (2), β-stigmasterol/β-sitosterol (mixture3a/b) and D-mannitol (4). The minimum inhibitory concentrations (MICs) ranged from 250 to > 500 μg/mL for extracts and fractions and from 15 to 250 μg/mL for compounds. In the presence of sub-inhibitory concentrations of the compounds, the MIC of amoxicillin againstE. coli(20 μg/mL) andP. aeruginosa(320 μg/mL) was reduced by 32 and 10 folds respectively. The whole extract demonstrated anti-biofilm formation and efflux pump inhibition inE. coli,S. aureusandP. aeruginosa. The sterol mixture (3a/b) at concentration of 100 μg/mL caused the highest inhibition (73%) of biofilm formation inS. aureus. Oleanonic acid (1) demonstrated remarkable efflux pump inhibition at MIC of 7.8 μg/mL inE. colibetter than the standard drugs verapamil and chlorpromazine.ConclusionThis study confirms the prospects ofA. genipifloraas a source of new antibacterial agents and adjuvants that could interact with some resistance mechanisms in bacteria to enhance the activity of hitherto ineffective antibiotics. “A small portion of the study has been presented in a conference in the form of poster”.
Next Generation Sequencing Should Be Proposed to Every Woman With “Idiopathic” Primary Ovarian Insufficiency
Authors: Eskenazi Sarah , Bachelot Anne , Hugon-Rodin Justine , Plu-Bureau Genevieve , Gompel Anne , Catteau-Jonard Sophie , Molina-Gomes Denise , Dewailly Didier , Dodé Catherine , Christin-Maitre Sophie , Touraine Philippe 
Published: March 2021

Abstract

Context
Primary ovarian insufficiency (POI) affects 1% of women under 40 years of age. POI is idiopathic in more than 70% of cases. Though many candidate genes have been identified in recent years, the prevalence and pathogenicity of abnormalities are still difficult to establish.

Objective
Our primary objective was to evaluate the prevalence of gene variations in a large prospective multicentric POI cohort. Our secondary objective was to evaluate the correlation between phenotype and genotype.

Methods
Two hundred and sixty-nine well-phenotyped POI patients were screened for variants of 18 known POI genes (BMP15, DMC1, EIF2S2, FIGLA, FOXL2, FSHR, GDF9, GPR3, HFM1, LHX8, MSH5, NOBOX, NR5A1, PGRMC1, STAG3, XPNPEP2, BHLB, and FSHB) by next generation sequencing (NGS). Abnormalities were classified as “variant” or “variant of unknown signification” (VUS) according to available functional tests or algorithms (SIFT, Polyphen-2, MutationTaster).

Results
One hundred and two patients (38%) were identified as having at least 1 genetic abnormality. Sixty-seven patients (25%) presented at least 1 variant. Forty-eight patients presented at least 1 VUS (18%). Thirteen patients (5%) had combined abnormalities. NOBOX variants were the most common gene variants involved in POI (9%). Interestingly, we saw no significant differences in the previous family history of POI, ethnic origin, age at onset of POI, primary amenorrhea, or secondary menstrual disturbances between the different genotypes.

Conclusion
In our study, a high percentage of patients presented gene variants detected by NGS analysis (38%). Every POI patient should undergo NGS analysis to improve medical cares of the patients.
La función ecológica de la propiedad y participación ambiental: dos promesas de la Constitución Ecológica de 1991
Authors: Jácome Parada Eliana María , Clavijo Mora Juliana Paola 
Published: March 2021

Con la Constitución de 1991, fueron introducidas una serie de normas protectoras en materia ambiental que han conducido a que se le denomine “constitución ecológica”. Dentro de estas, se identifican como preceptos de importancia significativa en el desarrollo de estos ideales, la función ecológica de la propiedad y la participación de la ciudadanía en materia ambiental.
En el artículo se estudiarán algunos casos conocidos por la Corte Constitucional, con el objetivo de analizar la forma en que en la práctica estas disposiciones ambientales constitucionales han sido aplicadas, tomando o no forma y dando cumplimiento a esa promesa de una constitución ambiental. Dentro de las altas cortes, fue escogida la Corte Constitucional debido a que es la autoridad encargada de proteger y velar por el cumplimiento de las disposiciones constitucionales, del mismo modo que es la corporación con mayor incidencia social, dada la repercusión de sus debates. Mediante su análisis, se demostrará que existe una marcada tendencia de esta corte a proteger los derechos a la participación ambiental de las comunidades y la función ecológica de la propiedad.
Assessment of the effect of therapy in a rat model of glioblastoma using [18F]FDG and [18F]FCho PET compared to contrast-enhanced MRI
Authors: Bolcaen Julie , Descamps Benedicte , Deblaere Karel , De Vos Filip , Boterberg Tom , Hallaert Giorgio , Van den Broecke Caroline , Vanhove Christian , Goethals Ingeborg 
Published: March 2021

Objective
We investigated the potential of [18F]fluorodeoxyglucose ([18F]FDG) and [18F]Fluoromethylcholine ([18F]FCho) PET, compared to contrast-enhanced MRI, for the early detection of treatment response in F98 glioblastoma (GB) rats.

Methods
When GB was confirmed on T2- and contrast-enhanced T1-weighted MRI, animals were randomized into a treatment group (n = 5) receiving MRI-guided 3D conformal arc micro-irradiation (20 Gy) with concomitant temozolomide, and a sham group (n = 5). Effect of treatment was evaluated by MRI and [18F]FDG PET on day 2, 5, 9 and 12 post-treatment and [18F]FCho PET on day 1, 6, 8 and 13 post-treatment. The metabolic tumor volume (MTV) was calculated using a semi-automatic thresholding method and the average tracer uptake within the MTV was converted to a standard uptake value (SUV).

Results
To detect treatment response, we found that for [18F]FDG PET (SUVmean x MTV) is superior to MTV only. Using (SUVmean x MTV), [18F]FDG PET detects treatment effect starting as soon as day 5 post-therapy, comparable to contrast-enhanced MRI. Importantly, [18F]FDG PET at delayed time intervals (240 min p.i.) was able to detect the treatment effect earlier, starting at day 2 post-irradiation. No significant differences were found at any time point for both the MTV and (SUVmean x MTV) of [18F]FCho PET.

Conclusions
Both MRI and particularly delayed [18F]FDG PET were able to detect early treatment responses in GB rats, whereas, in this study this was not possible using [18F]FCho PET. Further comparative studies should corroborate these results and should also include (different) amino acid PET tracers.
Impact of Industry 4.0 Concept on the Levers of Lean Manufacturing Approach in Manufacturing Industries
Authors: Ghouat M , Haddout A. , Benhadou M. 
Published: March 2021

Industrial companies looking for permanent performance are facing challenges of reducing production costs, reducing customer delivery delays and improving their quality products, this lead them to improve their responsiveness and flexibility to meet the varying needs of customers. To cope with these constraints, several industrial companies have adopted the Lean Manufacturing (LM) concept, based on the Toyota production system, to reduce wastage according to a methodical and structured approach that has given this proof for several years, this approach currently finds its limits, since it is based on static data, while a dynamic approach, with real-time data on customer needs and production performance, will readjust the levers of Lean Manufacturing to improve its efficiency. This paper has been aimed to show that the concept Industry 4.0 incarnates the lean Manufacturing approach by feeding it by real-time data and a real-time analysis of Big Data in a Cyber Physical Production System (CPPS), in order to improve decision-making and readjust in real time the levers of the Lean Manufacturing approach
PENGETAHUAN SANITASI HYGIENE KARYAWAN F&B PRODUCT DAN F&B SERVICE DI ISOLA RESORT BANDUNG
Authors: Prameswardani Indira , Lasmanawati Elly , Juawedah Ade 
Published: March 2021

Sanitasi Hygiene merupakan salah satu bentuk upaya untuk menciptakan lingkungan yang aman, sehat, dan bebas dari pencemaran lingkungan, sehingga dapat mengurangi penyakit. Penelitian ini dilatar belakangi berdasarkan kesadaran sanitasi hygiene pada karyawan pada saat bekerja. Penjamah makanan disarankan memiliki pengetahuan praktis sanitasi hygiene termasuk pekerja FB di hotel. Pengetahuan sanitasi hygiene di dapat dari SOP yang ada pada perusahaan, sebagai acuan dalam melaksanakan kerja karyawan sesuai standar. Penelitian ini bertujuan untuk memperoleh informasi dan gambaran tentang Pengetahuan Sanitasi dan Hygiene karyawan FB Product dan FB Service di Isola Resort. Metode yang digunakan yaitu metode kuantitatif, dengan populasi seluruh karyawan FB Product dan FB Service, teknik yang digunakan adalah sensus sejumlah 9 responden, dan instrumen yang digunakan menggunakan tes. Temuan penelitian menunjukan bahwa tingkat pengetahuan sanitasi hygiene berada pada kategori baik dengan persentase sebesar 76%. Rekomendasi untuk responden diharapkan dapat mengaplikasikan sanitasi hygiene pada saat bekerja agar dapat menciptakan lingkungan bersih dan sehat. Penelitian selanjutya diharapkan dapat meneliti lebih dari satu sampel hotel yang ada di Kota Bandung
Pyrolysis-GC/MS Analysis of Fast Growing Wood Macaranga Species
Authors: Subagyono R.R. Dirgarini J.N. , Qi Ying , Chaffee Alan L. , Amirta Rudianto , Marshall Marc 
Published: March 2021

Py-GC/MS analysis of six different species of fast growing Macaranga wood has been studied. Flash pyrolysis was conducted at different temperatures (250-850 oC) under a flow of helium followed by GC/MS analysis of the products. The total pyrolysis yields of the six different species of Macaranga were mostly between 40 and 90% within the range of pyrolysis temperature applied. Pyrolysis of the woody biomass produced compounds which are mostly derived from thermal degradation or volatilization of lignin and cellulose/hemicellulose, the original major constituents of the biomass. The Py-GC/MS technique indicated that M. gigantea was the most potential species for biofuel production and the optimum pyrolysis temperature to produce high yields of bio-oil was 450 oC.
Features of the phenological phases of introduced strawberry cultivars in the conditions of the Orel region
Authors: Zubkova M. I. , Knyazev S. D. , Evtikhova I. E. 
Published: March 2021

Relevance.The study of the timing and duration of individual phases of the development of strawberry cultivars of different ecological and geographical origin in these climatic conditions is of great practical importance. The purpose of the study was the determination of the timing of the main phenophases of introduced strawberry cultivars in the Orel region, the compliance of the phenorhythms of the studied cultivars with climatic conditions, as well as the ranking of the cultivars by the terms of flowering and maturation.Methods and materials.The article presents the results of phenological observations for the period 2016-2019. 34 strawberry cultivars of domestic and foreign selection were studied. The research was carried out at the VNIISPK site of primary variety study.Results. Based on long - term observations, on the time of entry into the flowering and fruiting phase, the cultivars were ranked into early, medium and late-maturing. The influence of effective temperatures on the onset of phenophases was analyzed. The sum of effective temperatures necessary for the beginning of flowering of strawberry cultivars of different maturation periods in the conditions of the Orel region was determined. For the onset of strawberry flowering, it takes from126.68 to 260.37°effective temperatures. Early cultivars need the sum of effective temperatures of 126.68-197.55°, cultivars of middle maturation need 146.31-225.44°, late cultivars - 159.93-260.37 depending on the year of study. Fruiting occurs at the sum of effective temperatures in early cultivars from 351.73°C to 465.43°C, in middle-maturing cultivars from 390.96 to 535.44°C, in late-maturing cultivars from 450°C to 649°C.
Comparative characteristics of the biochemical composition of VIR eggplant collection in a greenhouse
Authors: Gashkova I. V. , Solovieva A. E. , Kurina A. B. 
Published: March 2021

Relevance.The presence of valuable biologically active substances, carbohydrates, organic acids and others in the eggplant fruits makes the culture one of the vegetables with the highest antioxidant activity. The VIR collection of eggplant includes 830 accessions from 70 countries of the world. The study of new acquisitions in the VIR collection presupposes a comprehensive assessment of the received material according to morphological, biological and economically valuable characteristics. The main objective of the study was to assess the variability of the biochemical parameters of egg-plant fruits in technical ripeness; as well as determination of the best accessions by the chemical composition of fruits and the content of biologically active substances.Materials and methods.The study of 19 accessions of eggplant accessions was carried out in 2020 in a winter greenhouse in Pushkin and Pavlovsk Laboratories of VIR (St. Petersburg). The morphological description of the accessions and the assessment for biological and economically valuable traits were carried out in accordance with the methodological guidelines and the VIR classifier. Biochemical analysis was carried out in the Department of Biochemistry and Molecular Biology of VIR in the phase of technical ripeness of fruits in terms of: dry matter content, sugars, total acidity, ascorbic acid, pigments and anthocyanins.Results.As a result of this study, the amplitude of variability in the content of dry matter (6.44- 8.68%), sugars (1.78-3.72%), ascorbic acid (5.92-21.08 mg/100 g), titrated acidity (0.10-0.31%), chlorophylls (0.52-15.13 mg/100 g), carotenoids (1.19-6.99 mg/100 g), β-carotene (0.11-0.52 mg/100 g) and anthocyanins (12.94-1031.40 mg/100 g) in eggplant fruits. Accessions with a high content of biologically active substances in fruits in technical ripeness were identified: Russian hybrids Bourgeois F1, Azhur F1; local accessions from Armenia: k-3156, k-3159, k-3161.
Content of biologically active substances in seedlings of cabbage of the genus Brassica L.
Authors: Ushakova O V. , Molchanova A. V. , Bondareva L. L. 
Published: March 2021

Relevance. Photosynthetic seedlings, as a model object, are interesting both in the study of nutritional value and the species response of a plant organism to the cultivation.Material and methods. The study was carried out on the basis of the laboratory analytical department of the FSBSI FSVC. The material of the research was sprouts of Brassica chinensis L., cv. Vesnyanka; Brassica oleraceae L. var. italica Plenck, cv. Tonus; Brassica oleracea L. convar. acephala DC., cv. Malinovka; Brassica oleracea var. gongylodes L., hybrid F1 Sonata and cv. Venskaya Belaya 1350; Brassica oleracea L. convar. capitata (L.) Alef. var. capitata L. f. rubra (L.) Thell., cv. Gako 741. The seeds of the 2018 harvest were germinated in filter paper cassettes using distilled water. The cassettes with seeds were placed in a thermostat under constant illumination and a temperature of 25°C. They have been germinated for 8 days, after which a biochemical analysis of the samples was carried out.Results. It was found out that the total content of water-soluble antioxidants in the studied of cabbage crops was in the range of concentrations from 3.58-4.57 mg-eq HA/g (wet weight). According to this indicator of 8-day-old seedlings, red cabbage variety Gako 741 was distinguished by the maximum values. The total content of antioxidants in the alcoholic extract ranged from 21.24 to 28.23 mg-eq HA/g (dry weight). The content of polyphenols – in the concentration range of 16.39-24.94 mg-eq of HA/g (dry weight). As a result of the research, direct correlations of the studied characteristics were revealed. A high correlation was shown between the content of water-soluble antioxidants and dry matter (r=0.72...0.98) and between the content of carotenoids and chlorophylls (r=0.98...0. 99).
Lipid Clinic is an Efficacious Model of Preventive Medicine
Authors: Blokhina A. V. , Ershova A. I. , Meshkov A. N. , Limonova A. S. , Mikhailina V. I. , Drapkina O. M. 
Published: March 2021

Aim. To characterize patients accessing lipid clinic and assess the efficiency of treatment in a specialized medical center.Material and methods. A retrospective analysis of the surviving medical records of outpatients who visited the lipid clinic of the National Research Center for Therapy and Preventive Medicine (Moscow, Russia) in 2011-2019 (n=675) was carried out. Cardiovascular risk (CVR) and target lipoproteins levels were evaluated in accordance with actual guidelines for the diagnostics and correction of dyslipidemias.Results. The mediana of lipid clinic patients age was 57 [46;65] years. Female persons attend lipid clinic more often (61.5%). 48.5% of patients had low density lipoprotein cholesterol (LDL-c) >4.9 mmol/L, 7.7% had triglycerides level >5.5 mmol/L. Most of the patients were diagnosed with type IIa hyperlipidemia (44,1%) or type IIb (28,0%). Inherited impaired lipid metabolism was diagnosed in 27.7% individuals. 12.7% of the patients had familial hypercholesterolemia, 57.4% – had secondary causes of impaired lipid metabolism. More than half of the patients (52.4%) had low or moderate CVR, 28.1% had a very high CVR. High or very high CVR individuals revisited the lipid clinic more often than people with lower risk (68.2% vs. 35.4%). Revisiting patients (25.4%) reached LDL-c targets more often (33.3% of very high CVR patients; 45.5% of moderate-risk people) than in ordinary outpatient practice. High-intensity statin therapy was recommended for 32% of patients, and combined lipid-lowering therapy – for 14.8%. Among very high CVR individuals, combined lipid-lowering therapy was prescribed for 38.5%. Given the lipid-lowering therapy prescribed in the lipid clinic, LDL-с<1.8 mmol/L and<1.5 mmol/L will be achieved at 40.7% and 32.9% of patients with very high СVR.Conclusion. Lipid clinic is an important part of the medical care system for long-term follow-up of patients with impaired lipid metabolism, and it is more efficient in achieving target values of lipids and correcting risk factors in comparison with the primary medical service.
The Management of Multicultural Resolution on Post- Transnationalism of Indonesian Islamic Education: Challenges for the Future
Authors: Thoyib Muhammad 
Published: March 2021

This article discusses the urgency of the post-transnationalism paradigm of Indonesian Islamic education in the multicultural resolution management perspective that its substance discusses at least three main streams of discourse, namely: first, the substance of the post-transnationalism paradigm of Indonesian Islamic education that can be interpreted as an ideology of Islamic education that promotes the importance of moderation values that live within the multiculturalism of this nation, both on madrassas, pesantren and IslamicHigher Education. Secondly, the challenge of multiculturalism in Indonesian Islamic educationis this archipelago's problem with the dimensions of social conflict, radicalism, and extreme fundamentalism, which negates the importance of respect for diversity. Third, the multicultural resolution management design of Indonesian Islamic education for world peace must at least carry three main agendas that are very important in realizing the post-transnationalism paradigm in the future, which includes: development of a humanistic curriculum, strengthening moderation of institutional management, and strengthening institutional scientific partnering networks.Artikel ini mengkaji urgensivitas paradigma post-transnasionalisme pendidikan Islam Indonesia dalam perspektif manajemen resolusi multikulturalisme dengan nilai-nilai harmoni sosial, toleransi, kemajemukan, kerukunan, sekaligus saling menghargai sebagai kontribusi modal sosial bagi terbangunnya perdamaian dunia. Subtansi artikel ini setidaknya membincang tiga arus utama diskursus yaitu: pertama, subtansi paradigma post-transnasionalisme pendidikan Islam Indonesia yang dapat dimaknai secara interpretatif sebagai ideologi pendidikan Islam yang mempromosikan pentingnya nilai-nilai moderasi yang hidup dalam aras multikulturasme kebangsaan, baik pada etalase pendidikan madrasah, pesantren maupun perguruan tinggi Islam keagamaannya. Kedua, tantangan multikulturalisme pendidikan Islam Indonesia merupakan problematika nusantara yang bersifat akumulatif historis yang berdimensi konflik sosial, radikalisme, dan fundamentalisme ekstrim yang menafikan pentingnya penghargaan atas kebhinekaan (diversity). Ketiga, desain manajemen resolusi multikulturalisme pendidikan Islam Indonesia untuk perdamaian nusantara dan dunia setidaknya harus mengusung 3 agenda utama yang sangat penting dalam kerangka mewujudkan paradigma post-transnasionalisme pendidikan Islam Indonesia yang ekselen di masa depan, yang mencakup: pengembangan kurikulum pendidikan Islam humanis, penguatan moderasi manajemen kelembagaan pendidikan Islam, serta penguatan jejaring keilmuan yang ditunjang dengan komitmen dan kerjasama keilmuan (knowledge partnering) dalam skala nasional, regional dan internasional.