bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of metal ions on lipopeptide secretion from Bacillus subtilis strain FJAT-4: Negative regulation by Ca2+
Authors: Chen Meichun , Zheng Meixia , Chen Yanping , Xiao Rongfeng , Zheng Xuefang , Liu Bo , Wang Jieping , Zhu Yujing 
Published: October 2021

Abstract

Aims
This study aimed to investigate the effect of metal ions on lipopeptide production by Bacillus subtilis strain FJAT-4 and the mechanism of negative regulation by Ca2+.

Methods and Results
The quantitative measurement of lipopeptides in response to K+, Na+, Mg2+ and Ca2+ addition was carried out by LC-MS. The contents of fengycin and surfactin varied within the range of 116.24–129.80 mg/L and 34.03–63.11 mg/L in the culture media containing K+, Na+ and Mg2+, while the levels were 0.86 and 0.63 mg/L in the media containing Ca2+. Ca2+ at a high concentration (45 mM) did not adversely affect the growth of strain FJAT-4, but caused significant downregulation of lipopeptide synthesis-related gene expression, corresponding to a decrease in lipopeptide production. This inhibition by Ca2+ was further investigated by proteomic analysis. In total, 112 proteins were upregulated and 524 proteins were downregulated in the presence of additional Ca2+ (45 mM). Among these differentially expressed proteins (DEPs), 28 were related to phosphotransferase activity, and 42 were related to kinase activity. The proteomics results suggested that altered levels of three two-component signal-transduction systems (ResD/ResE, PhoP/PhoR and DegU/DegS) might be involved in the control of expression of the fen and srfA operons of FJAT-4 under high calcium stress.

Conclusions
The Ca2+ at the high concentration (45 mM) triggers a decrease in lipopeptide production, which might be attributed to the regulation of three two-component signal-transduction systems ResD/ResE, PhoP/PhoR and DegU/DegS.

Significance and Impact of the Study
The regulatory effect of calcium on the expression of genes encoding lipopeptide synthetases can be applied to optimize the production of lipopeptides.
Lived experience of neuropsychiatric symptoms among females with mild cognitive impairment: A phenomenological study
Authors: Lin Rose S. Y. , Yu Doris S. F. , Li Polly W. C. , Chau Pui Hing , Lee Jung Jae 
Published: October 2021

AbstractAimsTo explore the lived experience of neuropsychiatric symptoms (NPS) among females with mild cognitive impairment (MCI).DesignA phenomenological study using individual, semi‐structured, telephone‐based interviews was conducted to explore how the NPS are aroused, evolve, and affect the overall well‐being, illness perception and the corresponding coping responses adopted by females with MCI.MethodsTwenty‐nine participants with MCI were recruited from the community setting in Hong Kong between March and October 2020. Interviews were audio‐recorded, transcribed verbatim, and analysed using an interpretative phenomenological analysis approach and constant comparison strategy.ResultsThree themes were identified: (1) living vigilant lives with threats and uncertainty, (2) snowballing into further negative emotions and (3) seeking outlets for negative emotions. Our findings suggested that the participants' cognitive, functional and social challenges aroused intense emotional responses such as depression, agitation and anxiety. These noting negative emotions were further perpetuated by maladaptive coping responses, unrealistic expectations from coping strategies and overwhelming disease burden. Various internal and external strategies were adopted to enhance emotional adaptation, of which adopting a positive attitude appeared to be the most promising strategy.ConclusionThis study shed light on the challenging experience of MCI. The cognitive afflictions and the resultant impacts on various life domains evoked a cluster of NPS. Support services need to enhance emotional adjustment through alleviating the various life stressors and strengthening the coping resources.ImpactElucidating the lived experience of NPS provides important insights into the development of a more effective, comprehensive and person‐centred care planning for the population with MCI. Holistic care planning should extend beyond cognitive health optimization into enhancing disease knowledge, improve emotional coping, rebuild self‐identity and bolstering social supports among this preclinical cohort.
Novel dental composite resin derived from rice husk natural biowaste: A systematic review and recommendation for future advancement
Authors: Lin Galvin Sim Siang , Cher Chia Yee , Cheah Kah Kei , Noorani Tahir Yusuf , Ismail Noor Huda , Ghani Nik Rozainah Nik Abdul 
Published: October 2021

AbstractObjectivesThis review aimed to summarize contemporary evidence related to dental composite resin derived from rice husk biowaste and its potential future advancement. This review paper included the techniques for synthesis, characterization, and preparation of rice husk composite resin. Focus was also given to the flexural strength and modulus, compressive strength, wear rate, hardness, surface roughness, color stability, polymerization shrinkage, degree of conversion, and their application onto root canal treated teeth.Material and MethodsA search of English peer‐reviewed literature (January 1960–February 2021) was conducted from electronic databases (PubMed Central, Cochrane, LILACS, Science Direct, Web of Science, SIGLE, EMBASE, EBSCO, Medline, and Google Scholar).Results11 articles and a book section were finally selected for qualitative analysis. Studies concluded that the physicomechanical properties and the color stability of rice husk dental composites showed comparable results to conventional dental composites. Incorporation of zirconia nanopowder into rice husk dental composite increased the compressive strength and hardness values, associated with lower shrinkage, a high degree of conversion, and improved fracture strength when applied on root canal treated teeth.ConclusionsDue to its low cost, eco‐friendliness, and acceptable clinical performances, rice husk dental composite resin can be considered as an alternative to conventional composites.Clinical SignificanceDental composite resin derived from rice husk silica demonstrated excellent performance, which could potentially substitute currently available composite resins. This review will give new insight to clinicians and researchers on the usage of natural biowaste mass in the field of dental restorative materials.
A digital workflow with the virtual enamel evaluation and stereolithographic template for accurate tooth preparation to conservatively manage a case of complex exogenous dental erosion
Authors: Luo Tian , Zhang Junjing , Fan Lin , Huang Yutian , Yu Jiayi , Yu Haiyang 
Published: October 2021

AbstractObjectiveThis article describes a digital workflow using virtual enamel evaluation and a stereolithographic template for accurate tooth preparation for a complex exogenous dental erosion.Clinical ConsiderationsA 22‐year‐old man with different degrees of defects on the labial surface in esthetic area was diagnosed as exogenous dental erosion. The residual undamaged enamel area and depth of defect were measured and analyzed accurately by creating a digital virtual patient based on the pretreatment data. According to the different conditions of residual enamel and tooth defect, the treatment plans of porcelain veneer, crown and composite resin were chosen for corresponding involved teeth. Based on the virtual wax‐up and the suggested material thickness, a template for tooth preparation was designed and three‐dimensional printed. This template together with a special bur indicating the reduction depth accurately guided the teeth preparation and achieved a long‐term effect.ConclusionsThe virtual enamel evaluation contributes to obtaining the appropriate corresponding treatment plan objectively. The stereolithographic template effectively meets the accuracy of tooth preparation, preserving the tooth hard tissue to the greatest extent.Clinical SignificanceThe digital workflow described here may provide a quantifiable evaluation method and an accurate tooth preparation method for exogenous dental erosion.
Diet and gut microbiome in gastrointestinal disease
Authors: Trakman Gina L. , Fehily Sasha , Basnayake Chamara , Hamilton Amy L. , Russell Erin , Wilson‐O'Brien Amy , Kamm Michael A. 
Published: October 2021

AbstractThe composition and function of the dynamic microbial community that constitutes the gut microbiome is continuously shaped by the host genome, mode of birth delivery, geography, life stage, antibiotic consumption, and diet. Diet is one of the most potent factors in determining microbiome integrity. Dietary factors in early life appear to substantially determine the risk of later health or disease; for example, exposure to ultra‐processed foods in childhood or adolescence may increase the risk of the later development of inflammatory bowel disease or colorectal cancer, thought to be mediated by modulation of the gut microbiota. Dietary factors when gut diseases are established influence symptoms and disease activity, can form a risk factor for ongoing disease, or can be used as therapy to decrease disease activity. The characterization of dietary content is currently complex and imperfect, but tools are emerging to define precisely the nature of dietary composition. Similarly, the revolution in microbial analysis allows greater understanding of how diet influences microbial composition and function. Defining the interaction between diet, the gut microbiome, and gastrointestinal disease is leading to radical changes in our clinical approach to these disorders.
Awareness, acceptability, and satisfaction of teledermatology consultation among social‐media users in Nepal
Authors: Pokharel Sushan , Poudel Sishir , Agrawal Sudha , Marahatta Suchana 
Published: October 2021

AbstractBackgroundTeledermatology refers to the use of telecommunication in consulting one's skin problem to a dermatologist at distance.AimsThis study aims to identify the awareness, acceptability, preferred technical tools, willingness to use, comfortability, and satisfaction of teledermatology among social‐media users in Nepal.MethodsIt was an exploratory cross‐sectional study performed on 360 social‐media users, subjected to web‐based interview based on pretested proforma containing questions to address: awareness of telemedicine, type and tools preferred for teledermatology, willingness to use, comfortability, and satisfaction of teledermatology services.ResultsMost of the participants (60.8%) were initially unaware of telemedicine. Despite this, the majority (81.9%) were willing to use the services after being aware, mostly during the follow‐up (36.6%). Most of the participants (61.1%) preferred the hybrid model for teleconsultation, while mobile was the preferred technical tool (68.1%) mostly because of its compatibility (84.1%) and portability (56.3%). The majority of the participants felt that teledermatology consultation would be beneficial (77.2%) and they mostly preferred it directly from a dermatologist (85.6%). About two‐thirds (64.2%) also believed that teledermatology consultation would eliminate the need for a face‐to‐face consultation. The majority would be comfortable (68.1%) if their skin lesions are photographed and sent to a dermatologist and would be satisfied (62.5%) with the consultations, through Wi‐Fi and mobile data both (55.6%), and via Facebook (97.2%) and Viber (70.8%).ConclusionsDespite being unaware of telemedicine, the participants were willing to use teledermatology consultation and thought that they would be comfortable and satisfied with it.
Morphometric study of lymphangiogenesis in different lesions of psoriasis vulgaris with correlation to disease activity
Authors: Anis Nourhan , Assaf Magda , Diab Nagwa , Soliman Ahmed , Salah Eman 
Published: October 2021

AbstractBackground and objectivesIn the last decades, attention to the role of lymphangiogenesis in psoriasis has been paid. Our study was conducted to evaluate podoplanin‐stained lymphatic vessels and the level of lymphangiogenesis in papular psoriatic lesions and psoriatic plaques and ascertain if podoplanin provides any additional prognostic information.Materials and methodsNumber of lymphatic vessels and total lymphatic vessel area were morphometrically analyzed in podoplanin‐stained sections, using anti‐D2‐40, together with the immunohistochemical study of epidermal Ki‐67 in psoriasis vulgaris (n = 20) (papules = 7 and plaques = 13) and control skin specimens (n = 20).ResultsThe number of lymphatic vessels and total lymphatic vessel area were higher in psoriasis cases compared with normal skin (p = 0.01, p = 0.01 respectively). In psoriatic plaques, the number of lymphatic vessels, total lymphatic vessel area, and epidermal Ki‐67 immunoreactivity were higher than in papular lesions (p = 0.002, p = 0.008, and p = 0.01, respectively).ConclusionsPsoriasis vulgaris is found to be a lymphangiogenesis‐dependent disease, and the lymphatic vascular network is in remodeling and expanding process. Podoplanin may be implicated in the pathogenesis of psoriasis and could be used as a prognostic biomarker for disease severity and progression.
Determinants of seasonal influenza vaccination hesitancy among healthcare personnel: An integrative review
Authors: Hall Caroline M. , Northam Holly , Webster Adrian , Strickland Karen 
Published: October 2021

AbstractAims and objectivesTo explore the determinants of and behaviour change models for seasonal influenza vaccination compliance among healthcare personnel.BackgroundCOVID‐19 vaccine hesitancy among healthcare personnel may be better understood by exploring determinants of seasonal influenza vaccine hesitancy.DesignIntegrative literature review.MethodsA systematic search was conducted in accordance with PRISMA guidelines. Six thousand and forty‐eight articles were screened. Seventy‐eight met inclusion criteria. Due to the heterogeneity of included articles, a narrative synthesis was conducted utilising a conceptual matrix to identify thematic categories.ResultsSix thematic categories were identified as influencing HCP SIV compliance: ‘perceived vulnerability’, ‘trust’, ‘past behaviour’, ‘professional duty’, ‘access and convenience’ and ‘knowledge and experience’. The Health Belief Model (HBM) was the most commonly utilised health behaviour change model within the seasonal influenza vaccination context. Few studies have examined seasonal influenza vaccine acceptance and uptake within the Australian HCP context, particularly involving community care and aged care.ConclusionsFactors that appear to relate to influenza vaccination compliance among HCP can be grouped according to several thematic categories, and they also appear influential in COVID‐19 vaccine uptake. In particular, an emerging focus on ‘trust’ or the more emotive considerations of decision‐making around health‐protective behaviours requires further exploration in the context of a pandemic. Efforts to influence these domains to increase compliance, however, are likely to be impeded by a lack of a well‐developed and tested behaviour change model.Relevance to clinical practiceHealthcare personnel (HCP) face high levels of occupational exposure to seasonal influenza every year. An emerging focus on ‘trust’ and the more emotive considerations of decision‐making around health‐protective behaviours requires further exploration in the context of a pandemic.
Understanding relationships between general self‐efficacy and the healthy ageing of older people: An integrative review
Authors: Remm Sarah , Halcomb Elizabeth , Hatcher Deborah , Frost Steve A. , Peters Kath 
Published: October 2021

AbstractAims and objectivesThe aim of this integrative review was to investigate current literature exploring relationships between general self‐efficacy and the healthy ageing of older people.BackgroundEnhancing the health and well‐being of older adults, while mitigating consequences of illness and frailty are important priorities in healthy ageing. General self‐efficacy is closely associated with human behaviour and has been linked with improved health and well‐being.DesignAn integrative review using the five‐stage method described by Whittemore and Knafl (Journal of Advanced Nursing, 2005, 52, 546).MethodsAcademic databases CINAHL, MEDLINE and APA PsycInfo were searched between 2010 and 2020 for original, peer‐reviewed papers, published in English that investigated general self‐efficacy and factors associated with the healthy ageing of older people. Included papers were critically appraised using the Appraisal tool for Cross‐Sectional Studies (AXIS tool) and Critical Appraisal Skills Programme, and underwent data abstraction and synthesis via a constant comparative method. This review was also evaluated using the PRISMA checklist.ResultsTwenty‐one papers were included in this review. Two main themes emerged. The first highlights positive relationships between general self‐efficacy and health and ageing perceptions, with subsequent influence on health behaviours. The second includes two sub‐themes, which explores general self‐efficacy’s role in maintaining well‐being through its effects onpsychological healthandovercoming physical declinethrough adaption to changing physical and health conditions.ConclusionsPromoting general self‐efficacy has potential benefits for the healthy ageing of older people through positive effects on ageing and health perceptions, health behaviours, psychological health and overcoming physical decline.Relevance to clinical practiceUnderstanding how general self‐efficacy facilitates healthy ageing can guide nursing practices that reduce or mitigate consequences of illness and physical decline on the health and well‐being of older people. Strategies aimed at increasing older people’s general self‐efficacy can help to facilitate subsequent positive effects on factors that promote healthy ageing.
Primary uterine broad ligament ependymoma with CHEK2p.H371Y germline mutation: A CARE‐compliant case report uterine broad ligament ependymoma
Authors: Yin Jiaxin , Yao Min , Lu Hongdi , Cong Xiaofeng , Cui Pengfei , Liu Ziling 
Published: October 2021

AbstractEpendymomas arise from ependymal cells lining the ventricles and central canal of the spinal cord and can occur throughout the whole neuraxis. The lesion rarely occurs in extracranial or extraspinal regions, particularly in the uterine broad ligament. Thus, for the pathogenesis of nonsacral extra‐central nervous system (CNS) ependymomas remains elusive. Here, we describe a rare case of primary uterine broad ligament. ependymoma with cell‐cycle‐checkpoint kinase 2 (CHEK2) p.H371Y germline mutation. A 45‐year‐old woman presented with a uterine mass. The transvaginal sonographic examination confirmed a 4.4 cm × 3.7 cm, cystic and solid, mass located on the right side uterine wall near isthmus. First, laparoscopy with the neoplasm resection was carried out. Based on morphological and immunohistochemical characteristics of tumor cells that expressed glial fibrillary acidic protein (GFAP), S‐100, and vimentin, the tumor was diagnosed as an ependymoma. After that, she underwent a laparotomic total hysterectomy, bilateral salpingo‐oophorectomy, and lymphadenectomy. Furthermore, we performed next‐generation sequencing (NGS) of the patient's resected tumor tissue and peripheral blood and identified a novel CHEK2 p.H371Y germline mutation. Following surgery, the patient received oral tamoxifen (10 mg 2/day) and followed by letrozole (2.5 mg/day) for 6 months. The patient remained disease‐free after 4 years of follow‐up. Conceivably, CHEK2 p.H371Y is a driving gene for the development of extra‐CNS ependymoma.
Topical clonazepam for burning mouth syndrome: Is it efficacious in patients with anxiety or depression?
Authors: Rossella Intini , Alessandro Villa , Naman Rao , Gary Klasser , Hervé Sroussi Y. 
Published: October 2021

AbstractBackgroundBurning mouth syndrome (BMS) is a chronic disorder characterised by pain in the oral cavity without clinically evident causative lesions. The aetiology of this condition is poorly understood, and treatment can be challenging.MethodA retrospective review of the medical records of 82 patients with BMS was performed. Data on demographics, BMS associated symptoms, symptoms’ intensity score (NRS: 0–10) and response to treatment with topical clonazepam were extracted from clinical notes based on a standardised clinical questionnaire. Differences in the symptoms’ intensity score between patients with or without concomitant anxiety/depression or systemic psychogenic medication use were analysed using the Wilcoxon signed rank test.ResultsAmong the entire cohort, the median symptoms’ intensity score at baseline was 4.5 and 3.0 at first follow‐up, a statistically significant improvement (p < .001; 95% CI). Among the subjects with anxiety/depression and those who were prescribed systemic psychogenic medications, the median symptoms’ intensity score at baseline was 5.0 and 3.0 at first follow‐up, a statistically significant improvement (p < .001; 95% CI). Among those without anxiety/depression, the symptoms’ intensity score at baseline was 4.0 and 2.0 at first follow‐up, a statistically significant improvement (p < .05; 95% CI). The median symptoms’ intensity score for those who were not on any psychogenic medications at baseline was 4.0 and 2.0 at first follow‐up, a statistically significant improvement (p < .001; 95% CI).ConclusionsClinicians are encouraged to prescribe topical clonazepam for BMS regardless of concomitant use of systemic psychogenic medications or comorbid mood disorders as it is an efficacious management approach in the presence or absence of these potentially complicating factors.
Children's acceptability profiles for biscuits with different fiber content
Authors: da Quinta Noelia , Alvarez‐Sabatel Saioa , Martinez de Marañón Iñigo , Alfaro Begoña 
Published: October 2021

AbstractDue to the widespread rejection by children of products with high‐fiber content, new approaches to meet the dietary recommendations on fiber intake are necessary. To understand which sensory properties influence this rejection, children's acceptability was examined in high‐fiber biscuits and drivers of liking were identified. One hundred and ten Spanish children (6–12 years old) evaluated the overall liking of eight commercial biscuits with variable fiber content and stated their preference. To study the drivers of liking, the samples were characterized through a quantitative descriptive analysis, the determination of the moisture and water activity as well as the instrumental evaluation of texture with a texture analyzer. It was suggested that the addition of fiber in biscuits reduced children's liking ratings. High‐fiber samples were sensory and instrumentally described as harder, crispier, and more chewing than the samples with medium and low fiber content. The main sensory driver of liking identified in this study was the soft texture. Despite their hard texture, high‐ and medium‐fiber samples were chosen as the preferred ones for 14% of the children that participated when they included chocolate taste. Drivers of disliking identified in this study were related to the addition of fruit as a filling or as dehydrated pieces. This knowledge about children's acceptability of high‐fiber products might be of interest for the food industry with the aim of developing well‐accepted products that supply nutritional deficiencies associated with the fiber intake.