bluuebunny/crossref_metadata_2025_split huggingface dataset:
AMTSILATI METHOD IMPLEMENTATION IN PESANTREN AL-AQOBAH KWARON JOMBANG
Authors: Wicaksono Arif 
Published: September 2021

The purpose of this study was to determine the implementation of the amtsilati method at the Al-Aqobah Kwaron Islamic Boarding School, Jombang. This study concludes several points. First, the implementation of the Amtsilati Method at the Al-Aqobah Kwaron Diwek Jombang hut begins with mapping, namely: a) The first year of memorizing the rules of nahwu and sharaf science and understanding the five volumes of the Amtsilati book; b) The second year of implementing the Amtsilati method when reading a book that has been determined by the ustadz; c) The third year of reading, understanding the content of the yellow book with reference to the Amtsilati method. Second, the obstacle and solution in implementing the amtsilati method to accelerate the ability to read the yellow book at the Al-Aqobah Kwaron Diwek Jombang boarding school is the obstacle that students experience boredom when memorizing and understanding the Amtsilati method because of the different experiences of students before boarding and studying the yellow book. The solution is to add training so that those who are still left behind in the material can catch up with those who already understand giving lessons to those who cannot.
Keywords: amtsilati method, turath, pesantren.
IMPLEMENTASI METODE AMTSILATI DI PONDOK PESANTREN AL-AQOBAH KWARON JOMBANG
Abstrak
Tujuan penelitian ini adalah untuk mengetahui implementasi metode amtsilati di pondok pesantren al-aqobah kwaron jombang. Penelitian ini menyimpulkan beberapa poin. Pertama, Implementasi Metode Amtsilati di pondok Al-Aqobah Kwaron Diwek Jombang diawali dengan pemetaan yaitu: a) Tahun pertama menghafalkan qaidah ilmu nahwu dan ilmu sharaf dan memahami lima jilid kitab Amtsilati; b) Tahun kedua mengimplementasikan metode Amtsilati ketika membaca kitab yang telah ditentukan oleh ustadz; c) Tahun ketiga membaca, memahami kandungan kitab kuning dengan acuan metode Amtsilati. Kedua, Kendala dan solusi dalam implementasi metode amtsilati untuk mempercepat kemampuan membaca kitab kuning di pondok Al-Aqobah Kwaron Diwek Jombang adalah Kendala para santri mengalami kejenuhan ketika menghafal dan memahami metode Amtsilati karena pengalaman santri yang berbeda sebelum mondok dan belajar kitab kuning. Solusinya adalah penambahan latihan supaya yang masih tertinggal materi bisa menyusul yang sudah faham memberi pengajaran kepada yang belum bisa.
Kata kunci: metode amtsilati, turath, pesantren.
Low Molar Mass Dextran: One-Step Synthesis With Dextransucrase by Site-Directed Mutagenesis and its Potential of Iron-Loading
Authors: Wang Tingting , Jiang Zhiming , Wang Yiya , Wu Hao , Fang Yan , Dong Weiliang , Wu Bin , Ma Jiangfeng , Jiang Min 
Published: September 2021

Iron dextran is a common anti-anemia drug, and it requires low molar mass dextran as substrate. In this work, we selected 11 amino acid residues in domain A/B of DSR-MΔ2 within a 5-angstrom distance from sucrose for site-directed mutagenesis by molecular docking. Mutation of Q634 did not affect the enzyme catalytic activity, but showed an obvious impact on the ratio of low molecular weight dextran (L-dextran, 3,000–5,000 Da) and relatively higher molecular weight dextran (H-dextran, around 10,000 Da). L-dextran was the main product synthesized by DSR-MΔ2 Q634A, and its average molecular weight was 3,951 Da with a polydispersity index &lt;1.3. The structural characterization of this homopolysaccharide revealed that it was a dextran, with 86.0% α(1→6) and 14.0% α(1→4) glycosidic linkages. Moreover, L-dextran was oxidized with NaOH and chelated with ferric trichloride, and an OL-dextran-iron complex was synthesized with a high iron-loading potential of 33.5% (w/w). Altogether, mutation of amino acids near the sucrose binding site of dextransucrase can affect the chain elongation process, making it possible to modulate dextran size.
Rituximab and Abatacept Are Effective in Differential Treatment of Interstitial Lymphocytic Lung Disease in Children With Primary Immunodeficiencies
Authors: Rodina Yulia , Deripapa E. , Shvets O. , Mukhina A. , Roppelt A. , Yuhacheva D. , Laberko A. , Burlakov V. , Abramov D. , Tereshchenko G. , Novichkova G. , Shcherbina Anna 
Published: September 2021

BackgroundInterstitial lymphocytic lung disease (ILLD), a recently recognized complication of primary immunodeficiencies (PID), is caused by immune dysregulation, abnormal bronchus-associated lymphoid tissue (BALT) hyperplasia, with subsequent progressive loss of pulmonary function. Various modes of standard immunosuppressive therapy for ILLD have been shown as only partially effective.ObjectivesTo retrospectively evaluate the safety and efficacy of abatacept or rituximab in treatment of ILLD in children with PID.Methods29 children (median age 11 years) with various forms of PID received one of the two therapy regimens predominantly based on the lesions’ immunohistopathology: children with prevalent B-cell lung infiltration received rituximab (n = 16), and those with predominantly T-cell infiltration received abatacept (n = 17). Clinical and radiological symptoms were assessed using a severity scale developed for the study.ResultsThe targeted therapy with abatacept (A) or rituximab (R) enabled long-term control of clinical (A 3.4 ± 1.3 vs. 0.6 ± 0.1; R 2.8 ± 1 vs. 0.7 ± 0.05, p &lt; 0.01) and radiological (A 18.4 ± 3.1 vs. 6.0 ± 2.0; R 30 ± 7.1 vs. 10 ± 1.7, p &lt; 0.01) symptoms of ILLD in both groups and significantly improved patients’ quality of life, as measured by the total scale (TS) score of 57 ± 2.1 in treatment recipients vs. 31.2 ± 1.9 before therapy (p &lt; 0.01).ConclusionsILLD histopathology should be considered when selecting treatment. Abatacept and rituximab are effective and safe in differential treatment of ILLD in children.
Association Between Oxygen Partial Pressure Trajectories and Short-Term Outcomes in Patients With Hemorrhagic Brain Injury
Authors: Cai Guolong , Ru Weizhe , Xu Qianghong , Wu Jiong , Gong Shijin , Yan Jing , Shen Yanfei 
Published: September 2021

Objectives: Arterial hyperoxia is reportedly a risk factor for poor outcomes in patients with hemorrhagic brain injury (HBI). However, most previous studies have only evaluated the effects of hyperoxia using static oxygen partial pressure (PaO2) values. This study aimed to investigate the association between overall dynamic oxygenation status and HBI outcomes, using longitudinal PaO2 data.Methods: Data were extracted from the Medical Information Mart for Intensive Care III database. Longitudinal PaO2 data obtained within 72 h of admission to an intensive care unit were analyzed, using a group-based trajectory approach. In-hospital mortality was used as the primary outcomes. Multivariable logistic models were used to explore the association between PaO2 trajectory and outcomes.Results: Data of 2,028 patients with HBI were analyzed. Three PaO2 trajectory types were identified: Traj-1 (mild hyperoxia), Traj-2 (transient severe hyperoxia), and Traj-3 (persistent severe hyperoxia). The initial and maximum PaO2 of patients with Traj-2 and Traj-3 were similar and significantly higher than those of patients with Traj-1. However, PaO2 in patients with Traj-2 decreased more rapidly than in patients with Traj-3. The crude in-hospital mortality was the lowest for patients with Traj-1 and highest for patients with Traj-3 (365/1,303, 209/640, and 43/85 for Traj-1, Traj-2, and Traj-3, respectively; p &lt; 0.001), and the mean Glasgow Coma Scale score at discharge (GCSdis) was highest for patients with Traj-1 and lowest in patients with Traj-3 (13 [7–15], 11 [6–15], and 7 [3–14] for Traj-1, Traj-2, and Traj-3, respectively; p &lt; 0.001). The multivariable model revealed that the risk of death was higher in patients with Traj-3 than in patients with Traj-1 (odds ratio [OR]: 3.3, 95% confidence interval [CI]: 1.9–5.8) but similar for patients with Traj-1 and Traj-2. Similarly, the logistic analysis indicated the worst neurological outcomes in patients with Traj-3 (OR: 3.6, 95% CI: 2.0–6.4, relative to Traj-1), but similar neurological outcomes for patients in Traj-1 and Traj-2.Conclusion: Persistent, but not transient severe arterial hyperoxia, was associated with poor outcome in patients with HBI.
LncRNA KASRT Serves as a Potential Treatment Target by Regulating SRSF1-Related KLF6 Alternative Splicing and the P21/CCND1 Pathway in Osteosarcoma: An In Vitro and In Vivo Study
Authors: Chen Kai , Li Cheng , Huang Shuai , Chen Yu , Zhu Xiaodong 
Published: September 2021

PurposeLong non-coding RNA KLF6 alternative splicing regulating transcript (lnc-KASRT) locates within the intronic region of SRSF1, possessing the potential to regulate KLF6 alternative splicing to promote carcinogenicity. Then, the current in vitro and in vivo study aimed to investigate the effect of lnc-KASRT on regulating tumor malignant behaviors, and the implication of its interaction with KLF6 alternative splicing in osteosarcoma.MethodsLnc-KASRT overexpression or knockdown plasmid was transfected into U-2OS and Saos-2 cells. Then, KLF6-SV1 knockdown plasmid with or without lnc-KASRT overexpression plasmid was transfected into these cells for compensative experiments. In vivo, lnc-KASRT overexpression or knockdown Saos-2 cells were injected in mice for tumor xenograft construction.ResultsLnc-KASRT expression was increased in most osteosarcoma cell lines compared to control cell line. Lnc-KASRT overexpression promoted cell viability, mobility, and anti-apoptotic marker expression, while reducing apoptosis rate and pro-apoptotic marker expression; meanwhile, it regulated SRSF1, KLF6 alternative splicing (increased KLF6-splice variant 1 (KLF6-SV1), decreased KLF6-wild type (KLF6-WT)), and followed P21/CCND1 pathway in U-2OS/Saos-2 cells. The lnc-KASRT knockdown exhibited opposite trends. Subsequent compensative experiments disclosed that KLF6-SV1 knockdown attenuated most of the tumor-promoting effects of lnc-KASRT overexpression in U-2OS/Saos-2 cells. In vivo experiments further validated that lnc-KASRT enhanced tumor growth and reduced tumor apoptosis; meanwhile, it also increased tumor KLF6-SV1, MMP-1, and MMP-9 expressions but decreased tumor SRSF1 and KLF6-WT expressions in xenograft mice.ConclusionLnc-KASRT serves as a potential treatment target via regulating SRSF1-related KLF6 alternative splicing and following P21/CCND1 pathway in osteosarcoma.
Pre- and Early Post-treatment With Arthrospira platensis (Spirulina) Extract Impedes Lipopolysaccharide-triggered Neuroinflammation in Microglia
Authors: Piovan Anna , Battaglia Jessica , Filippini Raffaella , Dalla Costa Vanessa , Facci Laura , Argentini Carla , Pagetta Andrea , Giusti Pietro , Zusso Morena 
Published: September 2021

Background: Uncontrolled neuroinflammation and microglia activation lead to cellular and tissue damage contributing to neurodegenerative and neurological disorders. Spirulina (Arthrospira platensis (Nordstedt) Gomont, or Spirulina platensis), a blue-green microalga, which belongs to the class of cyanobacteria, has been studied for its numerous health benefits, which include anti-inflammatory properties, among others. Furthermore, in vivo studies have highlighted neuroprotective effects of Spirulina from neuroinflammatory insults in different brain areas. However, the mechanisms underlying the anti-inflammatory effect of the microalga are not completely understood. In this study we examined the effect of pre- and post-treatment with an acetone extract of Spirulina (E1) in an in vitro model of LPS-induced microglia activation.Methods: The effect of E1 on the release of IL-1β and TNF-α, expression of iNOS, nuclear factor erythroid 2–related factor 2 (Nrf2), and heme oxygenase-1 (HO-1), and the activation of NF-κB was investigated in primary microglia by ELISA, real-time PCR, and immunofluorescence.Results: Pre- and early post-treatment with non-cytotoxic concentrations of E1 down-regulated the release of IL-1β and TNF-α, and the over-expression of iNOS induced by LPS. E1 also significantly blocked the LPS-induced nuclear translocation of NF-κB p65 subunit, and upregulated gene and protein levels of Nrf2, as well as gene expression of HO-1.Conclusions: These results indicate that the extract of Spirulina can be useful in the control of microglia activation and neuroinflammatory processes. This evidence can support future in vivo studies to test pre- and post-treatment effects of the acetone extract from Spirulina.
Transoesophageal Ultrasound Assessment of Lung Aeration in Patients With Acute Respiratory Distress Syndrome
Authors: Brault Clément , Zerbib Yoann , Kontar Loay , Maizel Julien , Slama Michel 
Published: September 2021

Introduction: The effect of positive end-expiratory pressure (PEEP) depends closely on the potential for lung recruitment. Bedside assessment of lung recruitability is crucial for personalized lung-protective mechanical ventilation in acute respiratory distress syndrome (ARDS) patients.Methods: We developed a transoesophageal lung ultrasound (TE-LUS) method in which a quantitative (computer-assisted) grayscale determination served as a guide to PEEP-induced lung recruitment. The method is based on the following hypothesis: when the PEEP increases, inflation of the recruited alveoli leads to significant changes in the air/water ratio. Normally ventilated areas are hypoechoic because the ultrasound waves are weakly reflected while poorly aerated areas or non-aerated areas are hyperechoic. We calculated the TE-LUS re-aeration score (RAS) as the ratio of the mean gray scale level at low PEEP to that value at high PEEP for the lower and upper lobes. A RAS &gt; 1 indicated an increase in ventilated area. We used this new method to detect changes in ventilation in patients with a low (&lt;0.5) vs. high (≥0.5) recruitment-to-inflation (R/I) ratio (i.e., the ratio between the recruited lung compliance and the respiratory system compliance at low PEEP).Results: We included 30 patients with moderate-to-severe ARDS. In patients with a high R/I ratio, the TE-LUS RAS was significantly higher in the lower lobes than in the upper lobes (1.20 [1.12–1.63] vs. 1.05 [0.89–1.38]; p = 0.05). Likewise, the TE-LUS RAS in the lower lobes was significantly higher in the high R/I group than in the low R/I group (1.20 [1.12–1.63] vs. 1.07 [1.00–1.20]; p = 0.04).Conclusion: The increase in PEEP induces a substantial gain in the ventilation detected by TE-LUS of poorly or non-aerated lower lobes (dependent lung regions), especially in patients with a high R/I ratio.
Impact of Executive Functions and Parental Anxiety on the Development of Social Cognition in Premature Children: A Cross-Sectional Case-Control Protocol
Authors: Eutrope Julien , Novo Alexandre , Barbe Coralie , Loron Gauthier , Rolland Anne-Catherine , Caillies Stéphanie 
Published: September 2021

Background: Recent research has identified neuropsychological disorders, specifically executive function disorders, in premature children. Executive functions support goal-oriented mental activity and play a role in the development of social cognition. This underlies the social and emotional behavior of individuals. Parental anxiety is also an important environmental factor that can influence the psycho-emotional development of children.Objectives: The present protocol aims to compare the development of social cognition in school-age children born prematurely to that of school-age children born full-term, and to determine the impact of executive (dys)function and parental anxiety on such development.Methods/Design: In this cross-sectional protocol, 28 prematurely born children aged 7-10 years (“preterm”) and 28 full-term born children aged 7-10 years (“control”) will be included. The “preterm” and “control” groups will be matched for sex and age. The neuropsychological evaluation will include that of non-verbal intellectual efficiency (Raven's colored progressive matrices), verbal level (WISC-IV subtests), and executive functions (NEPSY II subtests and the opposite worlds of TEA-CH). The evaluation of social cognition will be conducted via tests of the theory of cognitive and affective mind. Several dimensions of the level of parental anxiety will be collected through the Spielberg Trait Anxiety Inventory Form Y, Beck Depression Inventory, Social Support Questionnaire-6, Parental Stress Index and, specifically for mothers, the Modified Perinatal Post-Traumatic Stress Disorder Questionnaire.Discussion: The results of this protocol will aid our understanding of the development of social cognition in premature children and to determine the factors that influence such development. This clinical research project, although following a fundamental approach, will have clinical implications because a more precise description of the development of social cognition in this school-age population will make it possible to better determine the cognitive targets of therapeutic actions and to search for predictive indices of the efficacy of practices.Trial Registration:https://clinicaltrials.gov/ct2/show/NCT03007095, identifier: NCT03007095.
Ten-Year Poverty Alleviation Effect of the Medical Insurance System on Families With Members Who Have a Non-communicable Disease: Evidence From Heilongjiang Province in China
Authors: Xia Qi , Wu Lichun , Tian Wanxin , Miao Wenqing , Zhang Xiyu , Xu Jing , Li Yuze , Shi Baoguo , Wang Nianshi , Yang Huiying , Huang Zhipeng , Yang Huiqi , Li Ye , Shan Linghan , Wu Qunhong 
Published: September 2021

Aims: Non-communicable diseases (NCD) drag the NCD patients' families to the abyss of poverty. Medical insurance due to weak control over medical expenses and low benefits levels, may have actually contributed to a higher burden of out-of-pocket payments. By making a multi-dimensional calculation on catastrophic health expenditure (CHE) in Heilongjiang Province over 10 years, it is significant to find the weak links in the implementation of medical insurance to achieve poverty alleviation.Methods: A logistic regression was undertaken to predict the determinants of catastrophic health expenditure.Results: The average CHE of households dropped from 18.9% in 2003 to 14.9% in 2013. 33.2% of the households with three or more NCD members suffered CHE in 2013, which was 7.2 times higher than the households without it (4.6%). The uninsured households with cardiovascular disease had CHE of 12.0%, which were nearly 10% points lower than insured households (20.4–22.4%). For Medical Insurance for Urban Employees Scheme enrolled households, the increasing number of NCD members raised the risk of impoverishment from 3.4 to 20.0% in 2003, and from 0.3 to 3.1% in 2008. Households with hospital in-patient members were at higher risk of CHE (OR: 3.10–3.56).Conclusions: Healthcare needs and utilization are one of the most significant determinants of CHE. Households with NCD and in-patient members are most vulnerable groups of falling into a poverty trap. The targeting of the NCD groups, the poorest groups, uninsured groups need to be primary considerations in prioritizing services that are contained in medical insurance and poverty alleviation.
Oral Spermine Supplementation in Gestated Rabbit: A Study on Villi Height of Immature Intestines
Authors: Tamba Riana Pauline , Moenadjat Yefta 
Published: September 2021

Introduction: Immature intestines are the major problem in prematurity. Postnatal oral spermine has been shown in studies to improve intestinal maturation in rats and piglets. This study aimed to find out the efficacy of spermine in rabbits during gestation.Method: An experimental study was done in an unblinded, randomized manner on those treated with and without spermine administration. A morphological examination of hematoxylin–eosin-stained villi was performed under a light microscope with a focus on villi height. Data were subjected to analysis.Results: The median of the spermine-treated group was found to be higher at 24, 26, and 28 days than the non-spermine group, but was not significantly different.Conclusion: Oral spermine supplementation during gestation might improve intestinal villi height in immature rabbit intestines.
EFEKTIFITAS MODUL BASIC AIRWAY MANAGEMENT BERBASIS GUIDED INQUIRY LEARNING MODEL TERHADAP KETERAMPILAN MAHASISWA KEPERAWATAN
Authors: Wisnu kanita Maria , Subekti Wulandari Ika , Suparmanto Gatot , Nur Kusumawati Heni 
Published: September 2021

Pembelajaran dalam praktik klinik merupakan komponen penting dalam pendidikan keperawatan mengingat keperawatan merupakan profesi berbasis praktik. Pembelajaran praktik klinik oleh mahasiswa keperawatan memerlukan adanya pengembangan metode yang dapat mempersiapkan mahasiswa untuk dapat mengasah keterampilannya. Tujuan penelitian ini adalah untuk mengetahui efektifitas modul basic airway management berbasis guided inquiry learning model terhadap keterampilan mahasiswa keperawatan. Penelitian ini adalah penelitian kuantitatif dengan quasi experimental dalam bentuk nonequivalent pretes-postest group design. 50 responden dipilih dengan teknik purposive sampling, 25 responden pada kelompok eksperimen, sedangkan 25 responden pada kelompok kontrol. Keterampilan sebelum perlakuan pada kelompok kontrol dinyatakan lulus sebanyak 3 responden (12%) dan kelompok intervensi sebanyak 6 responden (24%). Keterampilan setelah dilakukan perlakuan pada kelompok kontrol dinyatakan lulus sebanyak 11 responden (44%) dan kelompok intervensi sebanyak 15 responden (66%).
Data dalam penelitian dianalisis dengan uji Mann-Whitney. Hasil uji menunjukkan nilai p = 0,000 dimana hasil ini menyatakan bahwa Modul Berbasis Guided Inquiry Learning Model cukup efektif untuk meningkatkan keterampilan mahasiswa.
Learning in clinical practice is an important component in nursing education considering that it is a practice-based profession. Learning clinical practice by students requires the development of methods that can prepare students to hone their skills. The purpose of this study was to determine the effectiveness of the basic airway management module based on the guided inquiry learning model on the skills of students. This study was a quantitative study with a quasi-experimental form of nonequivalent pretest-posttest group design. 50 respondents were selected by purposive sampling technique, 25 respondents were in the experimental group, while 25 respondents were in the control group. Before receiving the treatments, 3 respondents (12%) in the control group and 6 respondents (24%) were passed the skills. After receiving the treatment, as many as 11 respondents (44%) in the control group and 15 respondents (66%) the intervention group could pass the skills. The data in the study were analyzed by the Mann-Whitney test. The test results showed the value of p = 0.000 which stated that the Guided Inquiry Learning Model-Based Module is quite effective for improving student skills.