bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the hub gene BUB1B in hepatocellular carcinoma via bioinformatic analysis and in vitro experiments
Authors: Fu Jie , Zhang Xiao , Yan Likun , Shao Yaoli , Liu Xinxu , Chu Yuan , Xu Ge , Xu Xundi 
Published: February 2021

Background
Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers and the fourth leading cause of cancer-related deaths in the world. Although the treatment of HCC has made great progress in recent years, the therapeutic effects on HCC are still unsatisfactory due to difficulty in early diagnosis, chemoresistance and high recurrence rate post-surgery.

Methods
In this study, we identified differentially expressed genes (DEGs) based on four Gene Expression Omnibus (GEO) datasets (GSE45267, GSE98383, GSE101685 and GSE112790) between HCC and normal hepatic tissues. A protein–protein interaction (PPI) network was established to identify the central nodes associated with HCC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the central nodes were conducted to find the hub genes. The expression levels of the hub genes were validated based on the ONCOMINE and Gene Expression Profiling Interactive Analysis (GEPIA) databases. Additionally, the genetic alterations of the hub genes were evaluated by cBioPortal. The role of the hub genes on the overall survival (OS) and relapse survival (RFS) of HCC patients was evaluated by Kaplan-Meier plotter. At last, the mechanistic role of the hub genes was illustrated by in vitro experiments.

Results
We found the following seven hub genes: BUB1B, CCNB1, CCNB2, CDC20, CDK1, MAD2L1 and RRM2 using integrated bioinformatics analysis. All of the hub genes were significantly upregulated in HCC tissues. And the seven hub genes were associated with the OS and RFS of HCC patients. Finally, in vitro experiments indicated that BUB1B played roles in HCC cell proliferation, migration, invasion, apoptosis and cell cycle by partially affecting mitochondrial functions.

Conclusions
In summary, we identified seven hub genes that were associated with the expression and prognosis of HCC. The mechanistic oncogenic role of BUB1B in HCC was first illustrated. BUB1B might play an important role in HCC and could be potential therapeutic targets for HCC.
Identifying the Activity Origin of a Cobalt Single‐Atom Catalyst for Hydrogen Evolution Using Supervised Learning
Authors: Liu Xinghui , Zheng Lirong , Han Chenxu , Zong Hongxiang , Yang Guang , Lin Shiru , Kumar Ashwani , Jadhav Amol R. , Tran Ngoc Quang , Hwang Yosep , Lee Jinsun , Vasimalla Suresh , Chen Zhongfang , Kim Seong‐Gon , Lee Hyoyoung 
Published: February 2021

AbstractSingle‐atom catalysts (SACs) have become the forefront of energy conversion studies, but unfortunately, the origin of their activity and the interpretation of the synchrotron spectrograms of these materials remain ambiguous. Here, systematic density functional theory computations reveal that the edge sites—zigzag and armchair—are responsible for the activity of the graphene‐based Co (cobalt) SACs toward hydrogen evolution reaction (HER). Then, edge‐rich (E)‐Co single atoms (SAs) were rationally synthesized guided by theoretical results. Supervised learning techniques are applied to interpret the measured synchrotron spectrum of E‐Co SAs. The obtained local environments of Co SAs, 65.49% of Co‐4N‐plane, 13.64% in Co‐2N‐armchair, and 20.86% in Co‐2N‐zigzag, are consistent with Athena fitting. Remarkably, E‐Co SAs show even better HER electrocatalytic performance than commercial Pt/C at high current density. Using the joint effort of theoretical modeling, thorough characterization of the catalysts aided by supervised learning, and catalytic performance evaluations, this study not only uncovers the activity origin of Co SACs for HER but also lays the cornerstone for the rational design and structural analysis of nanocatalysts.
Silk‐Inspired In Situ Hydrogel with Anti‐Tumor Immunity Enhanced Photodynamic Therapy for Melanoma and Infected Wound Healing
Authors: Tang Xiaoxuan , Chen Xiaoli , Zhang Siming , Gu Xinyi , Wu Ronghua , Huang Tingting , Zhou Zhihao , Sun Cheng , Ling Jue , Liu Mei , Yang Yumin 
Published: February 2021

AbstractEasy cancer recurrence and wound infections have been clinical challenges after surgical treatment of melanoma. Herein, a silk‐inspired in situ gelation system containing methacrylated silk fibroin (SF) and chlorine e6 for improved cancer therapy with enhanced wound healing is developed. Favored by the macrophage recruitment capacity of the SF hydrogel, promising antitumor immune responses can be turned “on” via near infrared irradiation in a controllable manner to achieve combination therapy with photodynamic therapy to significantly suppress melanoma recurrence. Moreover, the effective photodynamic antibacterial activity of this bioactive system with the capacity of light‐controllable modulating macrophage phenotype promotes remarkable tissue ingrowth with hair follicle regeneration for Staphylococcus aureus infected wound healing. Thus, this multifunctional silk‐based hydrogel system, as a desirable wound dressing, provides a new platform for promising melanoma therapy and skin regeneration.
An Affinity‐Based, Cysteine‐Specific ATP Analog for Kinase‐Catalyzed Crosslinking
Authors: Fouda Ahmed E. , Gamage Aparni K. , Pflum Mary Kay H. 
Published: February 2021

AbstractKinases mediate cell signaling pathways by catalyzing protein phosphorylation. Irregularities in kinase activity are directly associated with disease conditions. Therefore, methods to identify substrates of a particular kinase are needed to understand signaling cascades in normal and diseased states. Photocrosslinking ATP analogs provide powerful tools to study kinases by covalently linking kinases with substrates. However, the involvement of UV light and nonspecific reactivity of current ATP‐photocrosslinkers challenge kinase–substrate identification. We report here an affinity‐based crosslinking ATP analog, ATP‐methylacrylamide (ATP‐MAc), that contains a cysteine‐reactive acrylamide crosslinking group, which avoids the UV irradiation and non‐specific reactivity of prior analogs. Using in vitro kinase assays, ATP‐MAc acts as a kinase co‐substrate and covalently crosslinks only kinases containing cysteines in the active site. ATP‐MAc was also able to crosslink cellular proteins in lysates, documenting compatibility with cell‐based studies.
Steric Hindrance in Metal Coordination Drives the Separation of Pyridine Regioisomers Using Rhodium(II)‐Based Metal–Organic Polyhedra
Authors: Hernández‐López Laura , Martínez‐Esaín Jordi , Carné‐Sánchez Arnau , Grancha Thais , Faraudo Jordi , Maspoch Daniel 
Published: February 2021

AbstractThe physicochemical similarity of isomers makes their chemical separation through conventional techniques energy intensive. Herein, we report that, instead of using traditional encapsulation‐driven processes, steric hindrance in metal coordination on the outer surface of RhII‐based metal–organic polyhedra (Rh‐MOPs) can be used to separate pyridine‐based regioisomers via liquid–liquid extraction. Through molecular dynamics simulations and wet experiments, we discovered that the capacity of pyridines to coordinatively bind to Rh‐MOPs is determined by the positions of the pyridine substituents relative to the pyridine nitrogen and is influenced by steric hindrance. Thus, we exploited the differential solubility of bound and non‐bound pyridine regioisomers to engineer liquid–liquid self‐sorting systems. As a proof of concept, we separated four different equimolecular mixtures of regioisomers, including a mixture of the industrially relevant compounds 2‐chloropyridine and 3‐chloropyridine, isolating highly pure compounds in all cases.
ITQ‐69: A Germanium‐Containing Zeolite and its Synthesis, Structure Determination, and Adsorption Properties
Authors: Sala Andrés , Pérez‐Botella Eduardo , Jordá Jose L. , Cantín Angel , Rey Fernando , Valencia Susana 
Published: February 2021

AbstractIn this work, a new zeolite named as ITQ‐69, has been synthesized, characterized and its application as selective adsorbent for industrially relevant light olefins/paraffins separations has been assessed. This material has been obtained as pure germania as well as silica‐germania zeolites with different Si/Ge ratios using a diquaternary ammonium cation as organic structure directing agent. Its structure was determined by single‐crystal X‐Ray diffraction showing a triclinic unit cell forming a tridirectional small pore channel system (8×8×8R). Also, it has been found that Si preferentially occupies some special T sites of the structure as deduced from Rietveld analysis of the powder X‐ray diffraction patterns. In addition, the new zeolite ITQ‐69 has been found to be stable upon calcination and thus, its adsorption properties were evaluated, showing a promising kinetic selectivity for light olefin separations in the C3 fraction.
Doubling the Stakes: The Promise of Halide Double Perovskites
Authors: Wolf Nathan R. , Connor Bridget A. , Slavney Adam H. , Karunadasa Hemamala I. 
Published: February 2021

AbstractWhen the stakes are doubled in a wager, a player must correctly place two consecutive bets to win, but the payout is larger. Similarly, two B sites in combination dictate the properties of A2BB′X6 (A=monocation, X=halide) double perovskites. Correctly picking two B sites is more challenging than picking just one, as in the AIBIIX3 single perovskites, but the options are greater and, we believe, the rewards are higher when the stakes are doubled. In this Minireview, we emphasize fundamental aspects of halide double perovskites to provide a foundation for interested readers to explore this extraordinary class of materials. In particular, we highlight the differences and similarities between double and single perovskites and describe how the double perovskite structure potentially offers greater control over photophysical properties.
Metavalent Bonding in GeSe Leads to High Thermoelectric Performance
Authors: Sarkar Debattam , Roychowdhury Subhajit , Arora Raagya , Ghosh Tanmoy , Vasdev Aastha , Joseph Boby , Sheet Goutam , Waghmare Umesh V. , Biswas Kanishka 
Published: February 2021

AbstractOrthorhombic GeSe is a promising thermoelectric material. However, large band gap and strong covalent bonding result in a low thermoelectric figure of merit, zT≈0.2. Here, we demonstrate a maximum zT≈1.35 at 627 K in p‐type polycrystalline rhombohedral (GeSe)0.9(AgBiTe2)0.1 , which is the highest value reported among GeSe based materials. The rhombohedral phase is stable in ambient conditions for x=0.8–0.29 in (GeSe)1−x(AgBiTe2)x . The structural transformation accompanies change from covalent bonding in orthorhombic GeSe to metavalent bonding in rhombohedral (GeSe)1−x(AgBiTe2)x . (GeSe)0.9(AgBiTe2)0.1 has closely lying primary and secondary valence bands (within 0.25–0.30 eV), which results in high power factor 12.8 μW cm−1 K−2 at 627 K. It also exhibits intrinsically low lattice thermal conductivity (0.38 Wm−1 K−1 at 578 K). Theoretical phonon dispersion calculations reveal vicinity of a ferroelectric instability, with large anomalous Born effective charges and high optical dielectric constant, which, in concurrence with high effective coordination number, low band gap and moderate electrical conductivity, corroborate metavalent bonding in (GeSe)0.9(AgBiTe2)0.1. We confirmed the presence of low energy phonon modes and local ferroelectric domains using heat capacity measurement (3–30 K) and switching spectroscopy in piezoresponse force microscopy, respectively.
Cage‐Like Porous Materials with Simultaneous High C2H2Storage and Excellent C2H2/CO2Separation Performance
Authors: Di Zhengyi , Liu Caiping , Pang Jiandong , Chen Cheng , Hu Falu , Yuan Daqiang , Wu Mingyan , Hong Maochun 
Published: February 2021

AbstractAdsorption‐based separation is an important technology for C2H2purification due to the environmentally friendly and energy‐efficient advantage. In addition to the high selectivity of C2H2/CO2, the high uptake of C2H2also plays an important role in the separation progress. However, the trade‐off between adsorption capacity and separation performance is still in a dilemma. Herein, we report a series of cage‐like porous materials named FJI‐H8‐R (R=Me, Et,nPr andiPr) which all have high C2H2uptakes at 1 bar and 298 K. Dynamic breakthrough studies show that they all exhibit excellent C2H2/CO2separation performance. Particularly, FJI‐H8‐Me possesses a long breakthrough time up to 90 min g−1. Additionally, Grand Canonical Monte Carlo (GCMC) simulation reveals that the suitable pore space and geometry contribute much to the excellent separation performance.
Developmental timetables and gradients of neurogenesis in cerebellar Purkinje cells and deep glutamatergic neurons: A comparative study between the mouse and the rat
Authors: Martí‐Clúa Joaquín 
Published: February 2021

AbstractThe aim of this report is to determine whether the times of neuron origin and neurogenetic gradients of PCs and Deep cerebellar nucli (DCN) glutamatergic neurons are different between mice and rats. Purkinje cells (PCs) were analyzed in each compartment of the cerebellar cortex (vermis, paravermis, medial, and lateral hemispheres), and deep glutamatergic neurons at the level of the medialis, interpositus, and lateralis nuclei. Tritiated thymidine ([3H]TdR) autoradiography was applied on sections. The experimental rodents were the offspring of pregnant dams injected with [3H]TdR on embryonic days (E) 11–12, E12–13, E13–14, E14–15, E15–16, and E16–17. Our results indicate that systematic differences exist in the pattern of neurogenesis and the spatial location of cerebellar PCs and deep glutamatergic neurons between mice and rats. In mice, PCs and deep glutamatergic neurons neurogenesis extend from E10 to E14, with a predominance of neurogenesis on E12 for PCs, and on E12, E11, and E10 for the medialis, interpositus, and lateralis neurons, respectively. When neurogenesis in rats was considered, the data reveal that PCs and deep glutamatergic neurons production extends from E12 to E16, with a peak of production on E14 for PCs, and on E14, E13, and E12 for the medialis, interpositus, and lateralis neurons, respectively. Current data also indicate that, both in mice and rats, both types of macroneurons are generated according to a lateral‐to‐medial gradient. Thus, the lateral hemisphere and the lateralis nucleus present more early‐generated neurons than the vermis and the medialis nucleus, which in their turn have more late‐produced neurons.
A Hierarchically Porous ZIF@LDH Core‐Shell Structure for High‐Performance Supercapacitors
Authors: Zhao Zhimin , Duan Huiyu , Pang Huan , Zhu Rongmei 
Published: February 2021

AbstractDesigning nanocomposites with good electrochemical properties is one of the challenges in constructing supercapacitors. Adjustable metal‐organic frameworks (MOFs) have potential research value in improving charge storage and transfer due to their multi‐porosity. Moreover, MOFs can serve as a precursor to various derivatives. Herein, a series of core‐shell structures with macro‐microporous ZIF‐67 (M‐ZIF‐67) as the core and layered double hydroxide (LDH) as the shell were synthesized based on polystyrene spheres (PSs) template via a simple ion etching method. As a result, the sample of M‐ZIF‐67@LDH4 shows a specific capacitance of 597.6 F g−1 at 0.5 A g−1 and a high rate retention of 92% at 3 A g−1.
Through the Maze of Multivalent‐Ion Batteries: A Critical Review on the Status of the Research on Cathode Materials for Mg2+ and Ca2+ Ions Insertion
Authors: Maroni Fabio , Dongmo Saustin , Gauckler Cornelius , Marinaro Mario , Wohlfahrt‐Mehrens Margret 
Published: February 2021

AbstractThis review aims to address the status of transition metal‐based cathode materials for Mg2+ and Ca2+‐based multivalent‐ion batteries on a critical standpoint, providing a comprehensive overview. Multivalent‐based ions battery (MIB) technologies are among the most promising post‐lithium electrochemical energy storage devices currently studied, but they still fall short in several aspects due to their early stage of research. In addition, difficult experimental conditions related to the electrolyte systems and the cathode materials require an additional quote of care when performing experiments. In this review, a global approach is undertaken, from an introduction to electrolytes to the studied insertion parameters that allow a fast (de)insertion of multivalent ions. Then, the currently studied structural classes of cathode materials and a critical comment on data reporting, which are among the focal points of the actual state‐of‐the‐art research, are thoroughly discussed.
A forgotten diagnosis in right heart failure: A case report and literature review
Authors: Alizadehasl Azin , Galeshi Bahar , Peighambari Mehdi , Pouraliakbar Hamidreza , Moradian Maryam , Hashemi Arash 
Published: February 2021

AbstractCarcinoid heart disease is a well‐known complication of carcinoid syndrome that affects morbidity and mortality. Carcinoid heart disease may be asymptomatic in the early stages; therefore, patients with carcinoid syndrome should be screened to prevent misdiagnosis.
Novel Facile One‐Pot Synthesis of Bi2S3−BiOCl Ultrathin Hetero‐nanosheets for Selective Alcohol Oxidation
Authors: Yang Shouning , Liu Xiaoyang , Lu Sijia , Li Zhuo , Zhang Yanmin , Yu Shaoning , Song Jian , Ding Chuanfan , Yang Huayan 
Published: February 2021

AbstractThe development of eco‐friendly and low cost catalysts is challenging. Here, heterostructures of Bi2S3 quantum dots (QDs) doped onto ultrathin BiOCl nanosheets were synthesized by a facile hydrothermal method to exploit efficient photosensitizers with appropriate electronic states to enhance the transfer of electrons. The obtained Bi2S3−BiOCl showed highly efficient photocatalytic ability for selective oxidation of aromatic alcohols to aldehydes. More oxygen vacancies are formed on the exposed 001 facet of the ultrathin BiOCl, which can effectively trap electrons and form .O2− radicals. The cooperation between the BiOCl and the Bi2S3 QDs effectively separates photogenerated electron‐hole pairs at the heterointerface and facilitates the cooperative actions of .O2− radicals and holes, which brings about a desirable photocatalytic eﬃciency for the selective oxidation of aromatic alcohols. This work highlights the synergistic effect of semiconductor QDs and two‐dimensional materials on selective conversion processes and provides a new design paradigm using noble metal‐free heteromaterials with high photocatalytic activity. This opens new possibilities for photocatalyst design using heteromaterials with high photocatalytic activity.
Source Apportionment of Marine Microplastics: First Step Towards Managing Microplastic Pollution
Authors: Kumar Ashwini Suresh , Varghese George Kuttiparichel 
Published: February 2021

AbstractMonthly sampling from August 2018 to December 2019 was carried out in the beach sediments of Calicut to understand the monthly accumulation and deposition pattern of microplastics (MP). The average abundance in terms of items per kg dry sediment during monsoon months was higher than that of non‐monsoon months. The sources and pathways of the sampled microplastics were identified using an existing forensic framework. The pathways of pellets and fibers were seen as drainage outlet and harbor, respectively. Exactly identified sources include polycarbonate urethane (PCU) fragments from foam, polypropylene (PP) and polyethylene (PE)+PP films from paints of boats, and PE and PE+PP from fishing nets. The MP concentration in recreational areas was consistently higher except during flood periods.
IL‐22‐mediated renal metabolic reprogramming via PFKFB3 to treat kidney injury
Authors: Chen Wei , Shen Yilan , Fan Jiajun , Zeng Xian , Zhang Xuyao , Luan Jingyun , Wang Yichen , Zhang Jinghui , Fang Si , Mei Xiaobin , Zhao Zhen , Ju Dianwen 
Published: February 2021

AbstractKidney damage initiates the deteriorating metabolic states in tubule cells that lead to the development of end‐stage renal disease (ESTD). Interleukin‐22 (IL‐22) is an effective therapeutic antidote for kidney injury via promoting kidney recovery, but little is known about the underlying molecular mechanisms. Here, we first provide evidence that IL‐22 attenuates kidney injury via metabolic reprogramming of renal tubular epithelial cells (TECs). Specifically, our data suggest that IL‐22 regulates mitochondrial function and glycolysis in damaged TECs. Further observations indicate that IL‐22 alleviates the accumulation of mitochondrial reactive oxygen species (ROS) and dysfunctional mitochondria via the induction of AMPK/AKT signaling and PFBFK3 activities. In mice, amelioration of kidney injury and necrosis and improvement of kidney functions via regulation of these metabolism relevant signaling and mitochondrial fitness of recombinant IL‐22 are certificated in cisplatin‐induced kidney damage and diabetic nephropathy (DN) animal models. Taken together, our findings unravel new mechanistic insights into protective effects of IL‐22 on kidneys and highlight the therapeutic opportunities of IL‐22 and the involved metabolic regulators in various kidney diseases.
“Micropersonality” traits and their implications for behavioral and movement ecology research
Authors: Bailey Joseph D. , King Andrew J. , Codling Edward A. , Short Ashley M. , Johns Gemma I. , Fürtbauer Ines 
Published: February 2021

Abstract

Many animal personality traits have implicit movement‐based definitions and can directly or indirectly influence ecological and evolutionary processes. It has therefore been proposed that animal movement studies could benefit from acknowledging and studying consistent interindividual differences (personality), and, conversely, animal personality studies could adopt a more quantitative representation of movement patterns.

Using high‐resolution tracking data of three‐spined stickleback fish (Gasterosteus aculeatus), we examined the repeatability of four movement parameters commonly used in the analysis of discrete time series movement data (time stationary, step length, turning angle, burst frequency) and four behavioral parameters commonly used in animal personality studies (distance travelled, space use, time in free water, and time near objects).

Fish showed repeatable interindividual differences in both movement and behavioral parameters when observed in a simple environment with two, three, or five shelters present. Moreover, individuals that spent less time stationary, took more direct paths, and less commonly burst travelled (movement parameters), were found to travel farther, explored more of the tank, and spent more time in open water (behavioral parameters).

Our case study indicates that the two approaches—quantifying movement and behavioral parameters—are broadly equivalent, and we suggest that movement parameters can be viewed as “micropersonality” traits that give rise to broad‐scale consistent interindividual differences in behavior. This finding has implications for both personality and movement ecology research areas. For example, the study of movement parameters may provide a robust way to analyze individual personalities in species that are difficult or impossible to study using standardized behavioral assays.