bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspectives of Gynecologic Oncologists on Minimally Invasive Surgery During COVID-19 Pandemic: A Turkish Society of Minimally Invasive Gynecologic Oncology (MIJOD) Survey
Authors: dogan nasuh utku , Bilir Esra , Taskin Salih , Vatansever Dogan , Dogan Selen , Taskiran Cagatay , Celik Husnü , Ortac Fırat , Gungor Mete 
Published: May 2021

Abstract
Study ObjectiveTo evaluate gynecologic oncologists’ trends and attitudes towards the use of Minimally invasive surgery (MIS) in active period of the COVID-19 pandemic in Turkey.DesignOnline national survey sent to members of Turkish Endoscopy Platform consisting of six sections and 45 questions between the dates 1-15 June 2020 in Turkey to explore their surgical practice during the pandemic. SettingThree hospital types: Education and research hospital/university hospital, state hospital and private HospitalParticipantsGynecologic oncologists who are members of Turkish Endoscopy PlatformMeasurements and Results58 % of participants canceled all operations except for cancer surgeries and emergent operations. About a quarter of participants (28%) continued to operate laparoscopically and/or robotically. For the evaluation of the suspected adnexial mass (SAM) 64% used laparotomy and only 13 % operated by laparoscopy (L/S). For the management of low-risk early-stage endometrial cancer only fifth of the participants preferred to perform L/S. For endometrial cancer with high-intermediate risk factors more than half of participants preferred complete staging with laparotomy. For advanced stage ovarian cancer, one-fifth of the participants preferred to perform an explorative laparotomy, whilst 15 % preferred diagnostic laparoscopy to triage the patients for either NACT or cytoreductive surgery. On the contrary 41 % of participants chose to have cytology by paracentesis for neo-adjuvant chemotherapy (NACT). Gynecologic oncologists with >10 years L/S experience used MIS more for SAM. Furthermore, experienced surgeons used L/S more for endometrial cancer patients. In busy COVID hospitals, more participants preferred laparotomy over L/S. Conclusion﻿Use of MIS decreased during the pandemic in Turkey. More experienced surgeons continued to perform MIS. Surgical treatment was the preferred approach for SAM, early-stage endometrial cancer. However, NACT was more popular compared to radical surgery.
Exosome-Mediated Transfer of circRNA-GLIS3 Enhances Temozolomide Resistance in Glioma Cells Through miR-548m/MED31 Axis
Authors: Li Guowei , Jin YanPing 
Published: May 2021

Abstract
Background: TMZ resistance plays a critical role in the treatment of glioma, our research try to explore how circRNAs affect the chemosensitivity of glioma cells. Methods: In this study, we proceeded gene sequencing, and selected circRNAs specifically expressed in TMZ resistant cells and use them as target genes for subsequent studies; by knocking out the target gene we clarify its effect on TMZ resistant glioma proliferation, invasion, migration and cell apoptosis; through tumor-burdened animals we explore the effect of target gene in vivo environment. Results: In our research we revealed that circ-GLIS3 was significantly upregulated in TMZ resistant glioma cells. Functionally, knocking down circ-GLIS3 could inhibit proliferation, invasion, and migration abilities of TMZ resistant glioma cells; moreover, the downregulation of circ-GLIS3 could induce cell cycle arrest and apoptosis, while miR-548m inhibition and MED31 mRNA could reverse this progress. In vivo condition, the silencing of circ-GLIS3 could induce cell apoptosis and suppressed tumor growth. Mechanistically, circ-GLIS3 positively upregulated MED31 expression by sponging miR-548m. Conclusions: All these research findings demonstrate that circ-GLIS3 accelerates TMZ resistant glioma progression through miR-548m/MED31 axis.
Genetic regulation of DNA methylation across tissues reveals thousands of molecular links to complex traits
Authors: Oliva Meritxell , Demanelis Kathryn , Jasmine Farzana , Lu Yihao , Hahsan Habibul , Kibriya Muhammad , Chen Lin , Pierce Brandon 
Published: May 2021

Abstract
Epigenetic modifications of chromosomes, including DNA methylation (DNAm), play a fundamental role in gene regulation in humans. We generated DNAm data for 987 samples from the Genotype-Tissue Expression (GTEx) project, representing 9 tissue types and 424 subjects. We integrated GTEx RNA-seq data to examine methylome-transcriptome associations, their tissue specificity, and their overlap with regulatory regions. We mapped DNAm quantitative trait loci in cis (mQTLs), contrasted mQTLs with expression QTLs (eQTLs) with respect to functional elements, and assessed their relative contributions to complex traits. We identified thousands of mQTL links to traits in locations lacking a relevant eQTL. By integrating genetic, diverse -omics and phenotype data, we contribute to the understanding of molecular regulatory mechanisms in human tissues and their association with complex traits.
COVID-19 Vaccine Hesitancy in People With Migratory Backgrounds: A Cross-Sectional Study Among Turkish- and German-Speaking Citizens in Munich
Authors: Aktürk Zekeriya , Linde Klaus , Hapfelmeier Alexander , Kunisch Raphael , Schneider Antonius 
Published: May 2021

Abstract
BackgroundThis study aimed to investigate the knowledge, attitudes, behaviors, and vaccine hesitancy of people with migratory backgrounds among Turkish- and German-speaking patients in Munich regarding the COVID-19 pandemic.MethodsPrimary outcomes were the intention to get vaccinated for COVID-19 and COVID-19 knowledge levels (25 true/false items). Other variables included demographics, attitudes to COVID-19 and vaccination (7 items), and behaviors regarding COVID-19 (7 items). The attitude and behavior questions had 5-point Likert scales. Of the 10 Turkish-speaking family physicians in Munich, 6 agreed to administer Turkish or German questionnaires to consecutive patients during February 2021. Participants with either citizenship, country of origin, native language, or place of birth being non-German were categorized as “Having a migratory background.” Data from 420 respondents were analyzed.ResultsWomen constituted 41.4% (n=174), the mean age was 42.2±15.5 years, 245 (58.3%) preferred the Turkish questionnaire, 348 (82.9%) had a migratory background, and 197 (47.9%) intended to be vaccinated. The mean knowledge, attitude, and behavior scores were 21.5±3.2 (max=25), 3.7±0.8 (max=5), and 4.0±0.5 (max=5). While 42.3% (n=145) of the participants with a migratory background considered getting vaccinated, this proportion was 76.5% (n=52) for non-immigrant Germans (Chi-square=26.818, p<0.001). Non-migratory background (Odds Ratio (OR): 3.082), high attitude scores (OR: 2.877), male sex (OR: 2.185), years of schooling (OR: 1.064), and age (OR: 1.022) were positively associated with vaccination intention.ConclusionsWe suggest initiating or supporting projects run by persons or groups from inside the immigrants targeting to elaborate and change their vaccination attitudes.
Low Density Lipoprotein Cholesterol Is Associated With Increased Risk of Cardiovascular Disease in Participants Over 70 Years Old: A Prospective Cohort Study
Authors: Su xin , Zheng Deqiang , Wang Meiping , Zuo Yingting , Wen Jing , Zhai Qi , Zhang Yibo , He Yan 
Published: May 2021

Abstract
BackgroundPrevious studies, in which the data were collected about half a century ago, suggested that elevated low density lipoprotein cholesterol (LDL-C) is not associated with increased risk of cardiovascular disease (CVD) in patients over 70 years old. However, what is the relationship between LDL-C and CVD risk in a contemporary population aged over 70 years has not been well examined in China.MethodsThe China Health and Retirement Longitudinal Study (CHARLS) is an ongoing nationally representative study. In this analysis, participants of CHARLS who did not taking statins and did not have heart disease and stroke at 2011 were include and were followed up to 2018. The outcome of this analysis was occurrence of CVD at follow up, which include heart disease and stroke. Cox regression was used to assess the harmful effect of LDL-C on CVD occurrence. We calculated e-values to quantify the effect of unmeasured confounding on our results.ResultsOf the 9,631 participants, 15.2% (N=1,463) were aged over 70 years and 52.5% (N=5,060) were female. During the 7 years follow-up, 1,437 participants had a first CVD attack. Risk of CVD occurrence increased 8% with each 10 mg/mL elevation in LDL-C in whole participants (adjusted HR, 1.08; 95% CI, 1.06-1.10) and age groups of ≥70 years, 60-69years and <60 years. Similar results were observed in subgroup analyses, in which participants were stratified by sex, hypertension, diabetes and chronic kidney disease. According to the restricted cubic spline, we noted a U-shaped relationship between LDL-C and risk of CVD occurrence in group over 70 years old, however, we further found that in the left side of U-shape curve, LDL-C was not associated with occurrence of CVD and its attribution to CVD occurrence was only 2.1%, which indicated that lower level of LDL-C could not increase the risk of CVD occurrence as it was demonstrated by a U-shape association. E-value analysis suggested robustness to unmeasured confounding.ConclusionsIn contemporary society of China, elevated the level of LDL-C also increased the risk of CVD in participants over 70 years old as in the relatively younger participants. These results should strengthen guideline recommendations for the use of lipid lowering therapies in those elderly.
Triglyceride-glucose index in the development of peripheral artery disease: findings from the Atherosclerosis Risk in Communities (ARIC) Study
Authors: Gao Jing-Wei , Hao Qing-Yun , Gao Ming , Zhang Kun , Li Xiong-Zhi , Wang Jing-Feng , Vuitton Dominique A. , Zhang Shao-Ling , Liu Pin-Ming 
Published: May 2021

Abstract
Background

It remains unclear whether triglyceride-glucose (TyG) index, a surrogate marker of IR, was prospectively associated with incident PAD.
Methods

We included 12573 ARIC (Atherosclerosis Risk in Communities Study) participants free of PAD at baseline (1987–1989). The TyG index was determined using ln(fasting triglycerides [mg/dL] × fasting glucose [mg/dL]/2), and measured during 5 visits between 1987 and 2013. Incident PAD was defined as the first hospitalization with PAD diagnosis. We quantified the association of both baseline and trajectories of TyG index with incident PAD using Cox regression and logistic regression analysis, respectively.
Results

Over a median follow-up of 23 years, there were 1331 cases of incident PAD. After adjustment for traditional PAD risk factors, each 1-SD (0.58) increase in TyG index was associated with an 18.9% higher risk of incident PAD (hazard ratio, 1.189 [95% CI, 1.106–1.278]). Results were similar when individuals were categorized by TyG index quartiles (hazard ratio, 1.363 [95% CI, 1.125–1.652]; comparing extreme quartiles). Four distinct trajectories of TyG index were identified (low-increasing [43.0%], moderate-stable [22.3%], moderate-decreasing [27.6%], and high-decreasing [7.1%]). Trajectories of elevated TyG index levels had greater incident PAD after multivariable adjustment for potential cardiovascular risks. Compared with moderate-stable group (reference), high-decreasing group was associated with the highest risk of future incident PAD (odds ratio, 2.314 [95%CI, 1.687–3.175]).
Conclusion

Higher TyG index is independently associated with incident PAD. Long-term trajectories of TyG index help identify individuals at a higher risk of future PAD who deserve appropriate follow-up to detect asymptomatic disease.
Crosstalk between RPE cells and choroidal endothelial cells via the ANXA1/FPR2/SHP2/NLRP3 inflammasome/pyroptosis axis promotes choroidal neovascularization
Authors: Zhu Manhui , Wang Ying , Zhu Linling , Du Shu , Wang Zhenzhen , Zhang Yuting , Guo Yang , Tu Yuanyuan , Song E 
Published: May 2021

Abstract
One type of age-related macular degeneration (AMD), neovascular (nAMD), characterized by choroidal neovascularization (CNV), accounts for the majority of the severe central vision impairment associated with AMD. Endothelial cells (ECs) in direct contact with retinal pigment epithelial (RPE) cells are more prone to the pathological angiogenesis involved in CNV. Herein, we investigated the effect of crosstalk between RPE cells and choroidal endothelial cells (CECs) via the ANXA1/FPR2/NLRP3 inflammasome/pyroptosis axis on the development of choroidal neovascularization (CNV) in vitro and in vivo. ANXA1 expression and secretion from ARPE-19 cells were upregulated by hypoxia. FPR2 expression, especially on the plasma membrane, in HCECs was upregulated under hypoxic conditions. ANXA1 secreted from ARPE-19 cells inhibited NLRP3 inflammasome activation and NLRP3 inflammasome-mediated pyroptosis in HCECs by activating the FPR2/SHP2 axis. Moreover, ANXA1 secreted by ARPE-19 cells promoted behaviors of HCECs, including proliferation, migration and tube formation, by activating the FPR2/SHP2 axis and inhibiting NLRP3 inflammasome-mediated pyroptosis. Inhibiting the upregulated ANXA1/FPR2/SHP2/NLRP3 inflammasome/pyroptosis axis decreased the volume of CNV. Our data suggest that the crosstalk between RPE cells and CECs via the ANXA1/FPR2/NLRP3 inflammasome/pyroptosis axis promotes CNV. This finding could identify a potential target for the prevention and treatment of CNV.
Outcome and Prognostic Factors After Lung Transplantation for Bronchiectasis Other Than Cystic Fibrosis
Authors: Hirama Takashi , Tomiyama Fumiko , Notsuda Hirotsugu , Watanabe Tatsuaki , Watanabe Yui , Oishi Hisashi , Okada Yoshinori 
Published: May 2021

Abstract
BackgroundWhile lung transplant (LTX) can be an effective therapy to provide the survival benefit in selected populations, post-transplant outcome in LTX recipients with bronchiectasis other than cystic fibrosis (CF) has been less studied. Pseudomonas aeruginosa, often associated with exacerbations in bronchiectasis, is the most common micro-organism isolated from LTX recipients. We aimed to see the outcomes of patients with bronchiectasis other than CF after LTX and seek the risk factors associated with pre- and post-transplant Pseudomonas status.MethodsPatients who underwent LTX at Tohoku University Hospital between January 2000 and December 2020 were consecutively included into the retrospective cohort study. Pre- and post-transplant prevalence of Pseudomonas colonization between bronchiectasis and other diseases was reviewed. Post-transplant outcomes (mortality and the development of chronic lung allograft dysfunction(CLAD)) were assessed using a Cox proportional hazards and time-to-event outcomes were estimated using the Kaplan-Meier method. ResultsLTX recipients with bronchiectasis experienced a high rate of pre- and post-transplant Pseudomonas colonization compared to other diseases with statistical significance (p<0.001 and p<0.001, respectively). Nevertheless, long-term survival in bronchiectasis was as great as non-bronchiectasis (Log-rank p=0.522), and the bronchiectasis was not a trigger for death (HR 1.62, 95% CI 0.63-4.19). On the other hand, the chance of CLAD onset in bronchiectasis was comparable to non-bronchiectasis (Log-rank p=0.221), and bronchiectasis was not a predictor of the development of CLAD (HR 1.88, 95% CI 0.65-5.40).ConclusionsDespite high prevalence of pre- and post-transplant Pseudomonas colonization, the outcome in lung transplant recipients with bronchiectasis other than CF was comparable to those without bronchiectasis.
Molecular Dynamics Study on Structural and Atomic Evolution Between Au and Ni Nanoparticles Through Coalescence
Authors: Li Bangquan , Li Jing , Su Xiaoqiang , Cui Yimin 
Published: May 2021

Abstract
Motivated by the structure evolution experiments of Janus NiAu nanoparticles (NPs), we present a detailed study on the thermodynamic evolution of Ni and Au NPs with different ratios of Au and Ni through the molecular dynamics (MD) simulations. It is found that, for fixed Ni particle size (5.8 nm in diameter), the energy variation with the increasing temperature is related to the Au sizes (1.5–9.6 nm in diameter), due to the diverse atomic segregation modes. For a small Au particle, due to lattice induction, the structure will change from order to disorder and then to order. The interface defects of the merging NPs could be automatically eliminated by coalescence processes. The change in energy as the temperature increases is similar to that of monometallic NPs. For larger Au particles, the irregular variation of energy occurs and the atomic energy experience one or two reductions with the increase of the temperature. The segregation of Au atoms to the surface of Ni particle is dominant during the continuous heating process. The coalescence processes of Au atoms strongly determine the final morphology of the particles. Dumbbell-like, Janus and eccentric core-shell spherical structures could be obtained during the heating process. Our results will provide an effective approach to the design of novel materials with specific properties through thermal control.
Personalized Bioconversion of Panax Notoginseng Saponins Mediated by Gut Microbiota Between Chinese-diet and Western-diet Healthy Subjects
Authors: Wang Li , Chen Man-Yun , Shao Li , Zhang Wei , Li Xiang-Ping , Huang Wei-Hua 
Published: May 2021

Abstract
Background: Panax notoginseng saponins (PNS) as the main effective substances from P. notoginseng with low bioavailability could be bio-converted by human gut microbiota. In our previous study, PNS metabolic variations mediated by gut microbiota have been observed between high fat, high protein (HF-HP)-diet and low fat, plant fiber-rich (LF-PF)-diet subjects. In this study, we aimed to correspondingly characterize the relationship between distinct gut microbiota profiles and PNS metabolites. Methods: Gut microbiota were collected from HF-HP and LF-PF healthy adults, respectively and profiled by 16S rRNA gene sequencing. PNS were incubated with gut microbiota in vitro. A LC-MS/MS method was developed to quantify the five main metabolites yields including ginsenoside F1 (GF1), ginsenoside Rh2 (GRh2), ginsenoside compound K (GC-K), protopanaxatriol (PPT) and protopanaxadiol (PPD). The selected microbial species, Bifidobacterium adolescentis and Lactobacillus rhamnosus, were employed to metabolize PNS for the corresponding metabolites.Results: The five main metabolites were significantly different between the two diet groups. Compared with HF-HP group, the microbial genus Blautia, Bifidobacterium, Clostridium, Corynebacterium, Dorea, Enhydrobacter, Lactobacillus, Roseburia, Ruminococcus, SMB53, Streptococcus, Treponema and Weissella were enriched in LF-PF group, while Phascolarctobacterium and Oscillospira were relatively decreased. Furthermore, Spearman’s correlative analysis revealed gut microbiota enriched in LF-PF and HF-HP groups were positively and negatively associated with PNS metabolites yields, respectively. Conclusions: Our data showed gut microbiota diversity led to the personalized bioconversion of PNS.
Efficacy of Immunotherapy in Advanced Renal Cell Carcinoma (RCC) Patients Despite the Early Treatment Interruption Due to Serious Immune-related Adverse Events. Meet-Uro 13 Trial on Behalf of the MeetUro Investigators
Authors: Stellato Marco , Procopio Giuseppe , De Giorgi Ugo , Maruzzo Marco , Bimbatti Davide , Mennitto Alessia , Sbrana Andrea , Roviello Giandomenico , Casadei Chiara , Sepe Pierangela , Pignata Sandro , Santini Daniele 
Published: May 2021

Abstract
BackgroundSevere immune-related Adverse Events (irAEs) develop in 10-27% of patients treated with anti-Cytotoxic T-Lymphocyte Antigen (CTLA)-4, in amount 12-20% of patients treated with anti-programmed cell death (PD)-1 and 15-20% of patients treated with anti-programmed death-ligand (PD-L)-1(1–3). The aim of our study was to evaluate efficacy and clinical outcome of metastatic renal cell carcinoma (mRCC) patients who stopped Immune Checkpoint Inhibitors (ICIs) due to early Grade (G) 3-G4 irAEs.MethodsWe retrospectively collected data from 204 mRCC patients treated with ICIs in 6 Italian referral centers adhering to the Meet-Uro group, between February 2017 and January 2020. To properly weight the results, patients who did not report early G3-G4 toxicities have been included as control group.Primary endpoint was to evaluate 6 months Progression Free Survival (PFS) after early treatment interruption for Grade (G) 3-4 toxicities compared to the control group. Secondary endpoints were to evaluate Time to treatment failure (TTF) and overall survival (OS) in both groups. All statistical analyses were performed using SPSS software (version 19.00, SPSS, Chicago).Results18/204 (8.8%) patients had early treatment interruption for serious (G3-G4) irAEs. Early was defined as interruption of IO after one or two administrations. Immune related nephritis and pancreatitis were the most common irAE that lead to treatment interruption. 6/18 patients received IO-IO combination whereas 12/18 patients antiPD1. In patients who interrupted treatment due to irAE, 12/18 (66.6%) were free from progression at 6 months from IO interruption, TTF was 1.6 months (95%CI 1.6-2.1), mPFS was 7.4 months (95%CI 3.16-11.6) and mOS was 15.5 months (5.1-25.8). In the control group 111/184 (60.3%) patients were free from progression at 6 months, TTF was 4.6 months (95%CI 3.5-5.6), mPFS was 4.6 months (95%CI 3.5-5.6) and mOS was 19.6 months (95%CI 15.1-24.0). In the overall population, mPFS was 5.0 months (95%CI 4.0-5.9) and mOS was 19.6 months (95%CI 15.1-24.0). ConclusionsICIs seem to maintain efficacy even after early interruption due to severe irAE.
Research on CNN Coal and Rock Recognition Method Based on Hyperspectral Data
Authors: Yang Jianjian , Chang Boshen , Zhang Yuchen , Luo Wenjie , Wu Miao 
Published: May 2021

Abstract
Aiming at the problem of coal gangue identification in the current fully mechanized mining face and coal washing links, this article proposes a CNN coal and rock identification method based on hyperspectral data. First, collect coal and rock spectrum data by a near-infrared spectrometer, and then use four methods such as first-order differential (FD), second-order differential (SD), standard normal variable transformation (SNV), and multi-style smoothing to filter the 120 sets of collected data. The coal and rock reflectance spectrum data is preprocessed to enhance the intensity of spectral reflectance and absorption characteristics, and effectively remove the spectral curve noise generated by instrument performance and environmental factors.Construct a CNN model, judge the pros and cons of the model by comparing the accuracy of the three parameter combinations, select the most appropriate learning rate, the number of feature extraction layers, and the dropout rate, and generate the best CNN classifier for hyperspectral data. Rock recognition. Experiments show that the recognition accuracy of the one-dimensional convolutional neural network model proposed in this paper reaches 94.6%, which is higher than BP (57%), SVM (72%) and DBN (86%). Verify the advantages and effectiveness of the method proposed in this article.