bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Dopamine-Modified Cellulose Acetate Ultrafiltration Membranes with Improved Separation and Antifouling Performances
Authors: Ma Xi , Guo Hanxiang , Wang Zhaofeng , Sun Nan , Huo Pengfei , Gu Jiyou , Liu Yang 
Published: September 2021

Abstract
Cellulose derivatives are the earliest and most widely used membrane materials due to its many excellent characteristics, especially chemical activity and biodegradability. However, the hydrophobic properties of cellulose acetate (CA) limited its development to some extent. To improve the inherent hydrophobic and antifouling properties of the CA membrane, CA was successfully modified with dopamine (CA-2,3-DA) through selective oxidation and Schiff base reactions in this work, which was confirmed by 1H NMR and FTIR measurements. And then, the CA-2,3-DA membrane with high water permeability and the excellent antifouling property was prepared by the phase inversion method. Compared with the primordial CA membrane, the CA-2,3-DA membrane maintained a higher rejection rate for BSA (92.5%) while greatly increasing the pure water flux (167.3 L/m2h), which could be overcome the trade-off relationship between selectivity and permeability of the traditional CA membrane to a certain extent. According to the three-cycles dynamic ultrafiltration and static protein adsorption experiments, the CA-2,3-DA membrane showed good long-term performance stability and superior antifouling performance, which was supported by the experiment results including filtration resistance, flux decline ratio and flux recovery ratio. It is expected that this approach can greatly expand the high-value utilization of modified natural organic polysaccharides in separation engineering.
Repeated Intravenous Administration of hiPSC-MSCs Enhance The Efficacy of Cell-Based Therapy In Tissue Regeneration
Authors: Sun Si-Jia , Dong Ming , Lai Wing-Hon , Ho Wai-In , Wei Rui , Liang Wei-Hao , Li Fei , Liao Song-Yan , Tse Hung-Fat 
Published: September 2021

Abstract
We sought to demonstrate whether therapeutic efficacy can be improved by combination of repeated intravenous administration and local transplantation of human induced pluripotential stem cell derived MSCs (hiPSC-MSCs). In this study, mice model of hind-limb ischemia was established by ligation of left femoral artery. hiPSC-MSCs (5x105) was intravenously administrated immediately after induction of hind limb ischemia with or without following intravenous administration of hiPSC-MSCs every week or every 3 days. Intramuscular transplantation of hiPSC-MSCs (3x106) was performed one week after induction of hind-limb ischemia. We compared the therapeutic efficacy and cell survival of intramuscular transplantation of hiPSC-MSCs with or without a single or repeated intravenous administration of hiPSC-MSCs. Repeated intravenous administration of hiPSC-MSCs could increase splenic regulatory T cells (Tregs) activation, decrease splenic natural killer (NK) cells expression, promote the polarization of M2 macrophages in the ischemic area and improved blood perfusion in the ischemic limbs. The improved therapeutic efficacy of MSC-based therapy was due to both increased engraftment of intramuscular transplanted hiPSC-MSCs and intravenous infused hiPSC-MSCs. In conclusion, our study supported a combination of repeated systemic infusion and local transplantation of hiPSC-MSCs for cardiovascular disease.
Clinicopathological Characteristics and Treatment Outcomes of Occult Breast Cancer: A Population-Based Study
Authors: Zhao Zijun , Zhang Ting , Yao Yu , Lu Xin 
Published: September 2021

Abstract
Background
Occult breast cancer (OBC) is a special type of breast cancer. It is defined as an axillary lymph node metastasis without ipsilateral breast malignancy according to mammogram and ultrasonography. Because of its rarity and no primary lesion, clinicopathological information is still insufficient, causing a controversial condition about its treatment recommendation. In this study, we aimed to clarify major clinicopathological information, treatment strategies and prognosis of OBC based on a large population.
Methods
We retrospectively collected adult female OBC population from Surveillance, Epidemiology, and End Results (SEER) database. We divided the whole OBC cohort into two groups based on surgical treatment. Descriptive analysis of 18 clinicopathological variables was conducted by Wilcoxin rank sum test, Chi-square and Fisher’s exact test. Survival analysis was performed in the whole cohort as well as based on different clinicopathological factors. Univariate and multivariate Cox regression analysis was performed to identify potential independent predictor for prognosis of OBC.
Results
1189 OBC patients was included in final analysis. Elderly patients predominated with a mean age of nearly 60. Most of the OBC patients were diagnosed as an early-stage carcinoma and ductal carcinoma took up nearly 40% of all the histological types of OBC. Luminal A is the commonest subtypes of OBC. For in-breast treatment, number of patients received mastectomy was 4 times more than that of patients received breast-conserving surgery (BCS). More than 70% patients receiving BCS took radiotherapy, significantly more than the portion of patients receiving radiation in mastectomy group (50.9%, P < 0.001). Median follow-up period of all subjects was about 62 months. 5-year and 10-year overall survival (OS) of the complete OBC cohort was 81.6% and 68.8%, respectively. No significant difference in OS and breast-cancer specific survival (BCSS) was found between mastectomy and BCS. Older age and larger number of positive lymph nodes causes a worse prognosis whereas radiotherapy brought a better clinical outcome for OBC patients.
Conclusion
OBC is a rare breast malignancy with a generally good prognosis. Less-intensive surgical intervention does not negatively impact clinical outcomes of OBC while additional radiotherapy is totally beneficial to prolong OS and BCSS.
Evolution of an Increased Performance Under Acute Challenge Does Not Exacerbate Vulnerability to Chronic Stress
Authors: Lipowska Małgorzata M. , Sadowska Edyta T. , Palme Rupert , Koteja Paweł 
Published: September 2021

Abstract
An adequate stress response plays a vital role in coping with challenges. However, if selection for improved coping with an acute challenge affects the entire stress response system, susceptibility to adverse effects of chronic stressors can be deepened. Here, we used bank voles from lines selected for high swim-induced aerobic metabolism (A) and unselected control (C), and asked if the selection affected sensitivity to chronic mild stress (CMS). The voles were first habituated to daily weighing and feces collection for three weeks, and then for two weeks were exposed to CMS or remained undisturbed. The habituation itself resulted in an increased swim-induced oxygen consumption in both line types, and a decreased body mass. The CMS treatment caused reduction of food consumption in the second week of the experiment, and, in males, a decline in the metabolic rate. Paradoxically, fecal corticosterone metabolites decreased in the CMS-treated group. The response to CMS did not differ between the line types. Thus, the selection for increased performance was not traded off by increased vulnerability to chronic stress. However, the unexpected decrease in corticosterone suggests that bank voles – and perhaps also other animals – prefer experiencing unpredictable, unpleasant stressors over the monotony of standard laboratory housing.
Learning Approaches Among Medical Undergraduates and Pre-Intern Doctors of A Selected University In Sri Lanka
Authors: Edirisinghe Sajith , De Silva Dulmini , Liyanage Indunil , Niluka Malith , Madushika Kasuni , Deegodagamage Sandeepani , Wijesundara Chanuka , Dissanayake Harsha , Yasawardene Surangi 
Published: September 2021

Abstract
Background
Medical education is constantly evolving to suit the changes in the field. It is a challenge to acquire the necessary knowledge, attitudes and skills within a limited time period in order to become a proficient doctor. This study aims to determine and compare the learning approaches (deep, strategic, superficial) used by medical undergraduates and pre-intern doctors.
Methods
Learning approaches of 138 pre-clinical medical undergraduates and pre-intern doctors of the University of Sri Jayewardenepura were assessed using the Approaches and Study Skills Inventory for Students (ASSIST) questionnaire. Data were analyzed using SPSS v-16 and Brown-Forsythe test.
Results
The strategic approach was identified as the predominant learning approach among 138 participants. One hundred and eight (108) participants (78.3%) used this method while 21 (15.2%) and 9 (6.5%) participants used the deep approach and the surface apathetic approach, respectively. Majority of both undergraduates [77.6% (83/107)] and pre-interns [80.6% (25/31)] used the strategic approach. This finding was also consistent between the 2 genders with a majority of males [69.6% (32/46)] as well as females [82.6% (76/92)] following the strategic approach. No significant difference in learning approaches was found to be present between undergraduate and pre-intern groups.
Conclusions
This study demonstrates that a majority of medical undergraduates and pre-intern doctors prefer the strategic learning approach. This implies that the medical curriculum has not adequately promoted the deep learning approach over the five-year period of studentship and this needs to be addressed in a subsequent curriculum change in order to promote a deep learning approach.
Ensemble of Nucleic Acid Absolute Quantitation Modules for Accurate Copy Number Variation Detection and Targeted RNA Profiling
Authors: Wu Lucia , Dai Peng , Wang Michael , Chen Sherry , Cohen Evan , Jayachandran Gitanjali , Zhang Jinny , Serrano Angela , Ueno Naoto , REUBEN JAMES , Barcenas Carlos , Zhang David 
Published: September 2021

Abstract
Current gold standard for absolute quantitation of a specific DNA sequence is droplet digital PCR (ddPCR), which has been applied to gene copy number variation (CNV) detection. However, the number of quantitation modules in ddPCR has been limited by fluorescence channels, which thus limits the CNV sensitivity due to sampling error following Poisson distribution. Here we develop a PCR-based molecular barcoding NGS approach, quantitative amplicon sequencing (QASeq), for accurate absolute quantitation which is compatible with high multiplexing to include over 200 quantitation modules. By attaching barcodes to individual target molecules with high efficiency, 2-plex QASeq exhibits higher and more consistent conversion yield than ddPCR in absolute molecule count quantitation. Multiplexed QASeq improves CNV sensitivity to allow confident distinguishment of 2.05 ploidy from normal 2.00 ploidy. We applied multiplexed QASeq to serial longitudinal plasma cfDNA samples from patients with metastatic ERBB2+ (HER2+) breast cancer seeking association with tumor progression. We further show an RNA QASeq panel for targeted expression profiling on a wide range of RNA samples including tumor formalin-fixed, paraffin-embedded (FFPE) RNA.
Association between Meibomian Gland Dysfunction and Depressive Symptoms: A Multi-center Cross-sectional Study
Authors: Wei Zhenyu , Liang Junqi , Cao Kai , Wang Leying , Baudouin Christophe , Labbé Antoine , Liang Qingfeng 
Published: September 2021

Abstract
Increased prevalence of depression has been found in patients with meibomian gland dysfunction (MGD); however, specific conditions of patients suffered from MGD and depression remains unclear. Our aim was to investigate the prevalence of depression in patients with MGD and analyze the risk factors of depression in MGD patients. In this multi-center cross-sectional study, we enrolled 830 MGD patients and 114 normal controls. Demographic information was collected by designed questionnaires about lifestyle habits, systemic and ocular medical history. Ophthalmic examinations were performed in a formal order. Depression symptoms were evaluated with a questionnaire survey using a modified self-rating depression scale (M-SDS). The correlations between the M-SDS score and demographic and clinical information were analyzed. The prevalence of hyperlipidemia and obstructive sleep apnea-hypopnea syndrome (OSAHS) was higher in the MGD group. There were 78 cases (9.4%) with depressive symptoms in the MGD group whereas there were 4 cases (3.5%) in controls. Similarly, M-SDS was higher in the MGD group. The characteristics of depression in the MGD group included: crying spells, sleep disturbance and depressed appetite. Some systemic diseases (OSAHS, allergy, skin disease) and the use of contact lenses was correlated with an increased risk of depressive symptoms in MGD patients.
NPR3 inhibits EMT and Tumour Metastasis of Human Osteosarcoma by Activating AKT/mTOR-mediated Autophagy
Authors: Yao Zhihong , Yang Yihao , Chen Jiaxiang , Chen Ting , Han Lei , Li Dongqi , Ye Lijuan , Zhu Yanan , Feng Yan , Wu Jia , Yu Xinchao , Yang Zuozhang 
Published: September 2021

Abstract
BackgroundOsteosarcoma is an aggressive primary malignant cancer of bone mainly occurring in adolescence with a characteristic of high metastasis and relapse rate. In our previous study, we first identified that NPR3 was significantly decreased in OS samples. Here, we purposed to investigate the effect and the possible mechanisms of NPR3 on the progression of human OS. MethodsThe expression of NPR3 in OS patients and cells was detected by qRT-PCR, and IHC analysis. The effect of the expression of NPR3 on tumour metastasis was examined in vitro and in vivo. The molecular mechanisms of the regulation of NPR3 were evaluated in vitro and in vivo. The clinical relevance of 5-year overall survival with the expression of NPR3 was evaluated in 294 patients with OS. ResultsFirstly, we indicated that NPR3 was substantially downregulated expression in OS tissues and cells by qRT-PCR and IHC assay. And the patients with lower expression of NPR3 have a poor prognosis. Functional studies revealed that over-expression of NPR3 inhibited the proliferation and invasion of cells. Meanwhile, over-expression of NPR3 markedly inhibited tumorigenesis and weakened tumour metastasis in vivo. Interestingly, we found that over-expression of NPR3 could induce autophagy, promote apoptosis and inhibit EMT. Additionally, overexpression of NPR3 decreased the phosphorylation levels of AKT and mTOR. Loss-of-function experiments displayed that effects of NPR3 were weakened by treatment with the specific autophagy inhibitor Baf-A1 and CQ. ConclusionsTaken together, these results demonstrated that down-regulation of NPR3 promote lung metastasis of human OS by promoting EMT in part through the AKT/mTOR mediated autophagy, suggesting that NPR3 has therapeutic potential for OS patients with metastasis.
Concept and design of extended hybrid laminar flow control suction panels
Authors: Traub Hendrik , Wolff Johannes , Jose Siby , Lobitz Lennart , Schollerer Martin , Hühne Christian 
Published: September 2021

Abstract
Fully laminar aircraft are one step towards reaching eco-efficient aviation. However, high system complexity and significant manufacturing effort prevent the wide usage of existing laminarisation concepts such as laminar flow control, which are rarely found in commercial aircraft. Hybrid laminar flow control concepts reduce the manufacturing effort significantly at the cost of only achieving partial laminar flow. This paper presents extended hybrid laminar flow control concepts for fully laminar wings, with reduced system complexity. A detailed study of structural and aerodynamic requirements provides the foundation for partial design solutions of active suction structures. The authors derive two concepts for active suction panels from the structural design space. While the first concept relies on state of the art manufacturing techniques, the focus of the second concept is on additive manufacturing technologies. Based on these concepts, it is feasible to design fully laminar wings with structurally integrated active suction systems. The authors propose an aerodynamic test strategy for further developing extended hybrid laminar flow control.
Internet Industry Data Openness and Personal Information Protection Based on Privacy Laws
Authors: Gao Yurong , Guo Yiping , Jumani Awais Khan , Shankar Achyut 
Published: September 2021

Abstract
Data security needs a comprehensive system design approach that combines legal, administrative, and technical protection. These laws generally contain complete rules and principles relevant to the collecting, storing, and using personal information in line with international standards on privacy and data protection. Personal data should be legally collected for a specified reason and not be used without authorization for unlawful monitoring or profiling by governments or third parties. In advocacy and open data activity, increasing attention has been placed on privacy problems. To secure the protection of this data, the Privacy Law (PL) and the Regulations typically put forth industrial and technical standards on IT systems that hold and handle personal data. Concerns about information privacy are genuine, valid, and exacerbated on the Internet of Things (IoT) and Cyber-Physical Systems (CPS). This article suggests that compliance with IoT and CPS Data Privacy (DP) at technical and non-technical levels should be dealt with. The proposed architecture is then coupled with a reference framework for the business architecture to offer a DP-IoT model focused on the industry and technology and positioned to comply with the Personal Information Protection Act (POPI). Therefore, methods are necessary to protect data privacy based on both system and organizational reference designs. In the end, users should have specific rights to information about them, including the capacity and method to seek recourse to protect such rights, to acquire and amend incorrect details. The DP-IoT model shows a privacy ratio of 92.6%, scalability ratio of 91.5, data management ratio of 94.3%, data protection ratio of 96.7%, customer satisfaction rate of 92.2 %, attack prevention ratio of 95.5% and energy consumption ratio of 25.5 % compared to the existing methods.
DOA-based Localization Using Deep Learning for Wireless Seismic Acquisition
Authors: Aliyu Kabiru N. , Alawsh Saleh A. , Muqaibel Ali H. , Al-Dharrab Suhail I. , Mesbah Wessam , Reddy Varun A. , Bazzi Ahmad , Stüber Gordon L. 
Published: September 2021

Abstract
Oil and gas companies consider transforming conventional cable-based seismic acquisition to wireless acquisition as a promising step for cost and weight reduction in reservoir exploration. Wireless seismic acquisition requires large number of wireless geophone (WG) sensors to be deployed in the field. The locations of the WG sensors must be known when processing the collected data. The application of direction of arrival (DOA) estimation helps in localizing WGs and improves received signal level through beam steering and interference avoidance. Conventional DOA algorithms require high computational complexity which renders them inefficient for real-time response. In this paper, deep neural network (DNN) is proposed for DOA estimation of WGs at wireless gateway node (WGN) under different channel conditions. The estimated angle and corresponding coordinates of WGNs are used in least square estimation (LSE) to estimate the position of the WGs. The simulation results depict reasonable estimation and position accuracy in real-time.
Risk of Airway Fire with the use of KTP Laser and High Flow Humidified Oxygen Delivery in a Laryngeal Surgery Model
Authors: Huang Lucy , Badenoch Adam , Vermeulen Marthinus , Ullah Shahid , Woods Charmaine , Athanasiadis Theodore , Ooi Eng Hooi 
Published: September 2021

Abstract
Background Airway surgery presents a unique environment for operating room fire to occur. This study aims to explore the factors of combustion when using KTP laser with high flow oxygen in an ex-vivo model. MethodsThe variables tested were varying tissue type, tissue condition, oxygen concentration, laser setting, and smoke evacuation in a stainless-steel model. Outcome measures were time of lasing to the first spark and/or flame. A multivariate Cox proportional hazard model was used to determine the risk of spark and flame across the different risk factors. ResultsFor every 10% increase in oxygen concentration above 60% the risk of flame increased by a factor of 2.3. Continuous laser setting at 2.6W increased the risk by a factor of 72.8. The risk of lasing adipose tissue is 7.3 times higher than that of muscle. Charred tissue increases the risk of flame by a factor of 92.8. Flame occurred without a preceding spark 93.6% of the time. Conclusions Using KTP laser in the pulsed mode with low wattages, minimising lasing time, reducing the oxygen concentration and avoiding lasing adipose or charred tissue produce a relatively low estimated risk of spark or flame.
Tumor-Derived Biomimetic Nanozyme With Immune Evasion Ability For Synergistically Enhanced Low Dose Radiotherapy
Authors: Huang Chunyu , Liu Zeming , Chen Mingzhu , Du Liang , Zheng Yongfa , Liu Wei 
Published: September 2021

Abstract
High doses of radiation can cause serious side effects and drug resistance, high-performance radiosensitizers are urgently needed. To overcome this issue, we developed a biomimetic nanozyme system (CF) by coating pyrite (FeS2) into tumor-derived exosomes for use in combination with low-dose RT. CF system give FeS2 with immune escape and homologous targeting abilities. After administration, CF with both glutathione oxidase (GSH) and peroxidase activities can significantly lower the content of GSH in tumor tissues and catalyze intracellular hydrogen peroxide (H2O2) to produce a large amount of ·OH for intracellular redox homeostasis disruption and mitochondria destruction, thus reducing RT resistance. Experiments in vivo and in vitro showed that combining CF with RT (2Gy) can provide a substantial suppression of tumor proliferation. To summarize, this is the first attempt to use exosomes bionic FeS2 nanozyme for realizing low-dose RT, which broaden the prospects of nanozymes.