bluuebunny/crossref_metadata_2025_split huggingface dataset:
Connexin 43 phosphorylation by casein kinase 1 is essential for the cardioprotection by ischemic preconditioning
Authors: Hirschhäuser Christine , Lissoni Alessio , Görge Philipp Maximilian , Lampe Paul D. , Heger Jacqueline , Schlüter Klaus-Dieter , Leybaert Luc , Schulz Rainer , Boengler Kerstin 
Published: March 2021

AbstractMyocardial connexin 43 (Cx43) forms gap junctions and hemichannels, and is also present within subsarcolemmal mitochondria. The protein is phosphorylated by several kinases including mitogen-activated protein kinase (MAPK), protein kinase C (PKC), and casein kinase 1 (CK1). A reduction in Cx43 content abrogates myocardial infarct size reduction by ischemic preconditioning (IPC). The present study characterizes the contribution of Cx43 phosphorylation towards mitochondrial function, hemichannel activity, and the cardioprotection by IPC in wild-type (WT) mice and in mice in which Cx43-phosphorylation sites targeted by above kinases are mutated to non-phosphorylatable residues (Cx43MAPKmut, Cx43PKCmut, and Cx43CK1mut mice). The amount of Cx43 in the left ventricle and in mitochondria was reduced in all mutant strains compared to WT mice and Cx43 phosphorylation was altered at residues not directly targeted by the mutations. Whereas complex 1 respiration was reduced in all strains, complex 2 respiration was decreased in Cx43CK1mut mice only. In Cx43 epitope-mutated mice, formation of reactive oxygen species and opening of the mitochondrial permeability transition pore were not affected. The hemichannel open probability was reduced in Cx43PKCmut and Cx43CK1mut but not in Cx43MAPKmut cardiomyocytes. Infarct size in isolated saline-perfused hearts after ischemia/reperfusion (45 min/120 min) was comparable between genotypes and was significantly reduced by IPC (3 × 3 min ischemia/5 min reperfusion) in WT, Cx43MAPKmut, and Cx43PKCmut, but not in Cx43CK1mut mice, an effect independent from the amount of Cx43 and the probability of hemichannel opening. Taken together, our study shows that alterations of Cx43 phosphorylation affect specific cellular functions and highlights the importance of Cx43 phosphorylation by CK1 for IPC’s cardioprotection.
Adjusting residual stresses by flexible stress superposition in incremental sheet metal forming
Authors: Maaß Fabian , Hahn Marlon , Tekkaya A. Erman 
Published: March 2021

AbstractProcess-induced residual stresses significantly influence the mechanical properties of a formed component. A polymer pad is used as a flexible die in two-point incremental forming to induce compressive residual stresses in the component during the forming process. Experimental and numerical results illustrate the influence of compressive stress superposition on the component properties. It is shown that the active support, using a geometry-independent polyurethane die, causes beneficial compressive residual stresses on the tool side compared to the tensile residual stresses induced by the single-point incremental forming process without such a supporting die.
Anwendung der Induzierten Polarisation in der Graphitexploration
Authors: Flores-Orozco Adrián , Katona Timea , Aigner Lukas , Steiner Matthias , Römer Alexander 
Published: March 2021

ZusammenfassungDiese Studie präsentiert die Anwendung der Induzierten Polarisation (IP) für die Charakterisierung einer Graphitlagerstätte. Basierend auf Labormessungen zeigen wir die Sensitivität von IP-Messungen hinsichtlich Veränderungen der Korngröße und der spezifischen Oberfläche von Graphit in Gesteinsproben und auf der Feldskala. Des Weiteren demonstrieren wir, dass IP-Feldmessungen eine klarere Abgrenzung von graphitreichen Untergrundbereichen ermöglichen als die alleinige Bestimmung der elektrischen Leitfähigkeit.
Comparison of first-time microvascular decompression with percutaneous surgery for trigeminal neuralgia: long-term outcomes and prognostic factors
Authors: Noorani Imran , Lodge Amanda , Durnford Andrew , Vajramani Girish , Sparrow Owen 
Published: March 2021

Abstract
Objective
Common surgical treatments for trigeminal neuralgia (TN) include microvascular decompression (MVD) and percutaneous procedures (glycerol rhizolysis; thermocoagulation; and balloon compression). Although the efficacy of each procedure has been documented, direct comparisons of their relative efficacies for TN are lacking. We aimed to directly compare long-term outcomes after first-time MVD with percutaneous surgery in primary (idiopathic and classical) TN and identify predictors of outcome.

Methods
We conducted a retrospective analysis of prospectively collected data on 185 patients undergoing MVD and 129 undergoing percutaneous surgery. Procedures were performed by one of two neurosurgeons in a single centre; an independent observer collected long-term follow-up data by interviews, using the same outcome measures for all procedures.

Results
MVD patients were younger than those undergoing percutaneous surgery (P <.001). MVD provided superior initial pain relief (P <.001): 87.0% had Barrow Neurological Institute class I or II pain scores after MVD compared with 67.2% after percutaneous surgery. The complication rate for percutaneous procedures was 35.7% and for MVDs was 24.9% (P =.04), including minor and transient complications. Kaplan-Meier analysis demonstrated that MVD provided longer pain relief than percutaneous procedures (P <.001); 25% of patients had recurrence at 96 months following MVD compared with 12 months after percutaneous surgery. Subgroup analysis showed that balloon compression provided more durable relief amongst percutaneous procedures. Multivariate analysis revealed that post-operative numbness and age were prognostic factors for percutaneous procedures (P =.03 and .01, respectively).

Conclusions
MVD provides better initial pain relief and longer durability of relief than percutaneous surgery, although carrying a small risk of major complications. Amongst percutaneous procedures, balloon compression gave the most durable relief from pain. Older age and post-operative numbness were predictors of good outcome from percutaneous surgery. These results can help clinicians to counsel patients with primary TN on neurosurgical treatment selection for pain relief.
Periodontal manifestations of Langerhans cell histiocytosis: a systematic review
Authors: Difloe-Geisert Julia C. , Bernauer Selina A. , Schneeberger Noémie , Bornstein Michael M. , Walter Clemens 
Published: March 2021

Abstract
Objectives
To explore the evidence of periodontal manifestations and treatment modalities in patients with Langerhans cell histiocytosis (LCH).

Material and methods
A systematic literature search was performed and the criteria for PRISMA and risk of bias assessment were applied. Human clinical studies (≥10 patients) presenting patients with LCH and periodontal findings were considered for inclusion.

Results
From 298 titles identified, six case series with a total of 1278 patients suffering from LCH were included. In these studies, oral symptoms were reported in a frequency ranging from 10 to 100%. Overall, in 216 patients (17%), oral symptoms were observed. Out of these patients, 49–100% demonstrated periodontal symptoms. The most common oral findings were pain, swelling, tooth loss/mobility, and bone lesions. Specific periodontal findings comprised varying frequencies of gingival ulcerations, increased pocket depths, and gingival bleeding. Treatment measures constituted of surgical curettage of bone lesions, soft tissue excision and/or tooth extractions, radiotherapy, systemic chemotherapy, or a combination of these approaches. Healing without recurrence of oral lesions was reported in most of the cases.

Conclusions
The available evidence on periodontal manifestations in LCH patients is heterogeneous. Several oral and periodontal findings were reported and may occur as initial symptoms and/or at later stages of the disease.

Clinical relevance
The dentist should be aware of possible oral involvement of systemic diseases such as LCH, and these manifestations may mimic periodontal disease.
Synergistic Effects of Rooted Aquatic Vegetation and Drift Wrack on Ecosystem Multifunctionality
Authors: Austin Å. N. , Hansen J. P. , Donadi S. , Bergström U. , Eriksson B. K. , Sundblad G. , Eklöf J. S. 
Published: March 2021

AbstractEcosystem multifunctionality is an increasingly popular concept used to approximate multifaceted ecosystem functioning, which in turn may help advance ecosystem-based management. However, while experimental studies have shown a positive effect of diversity on multifunctionality, observational studies from natural systems—particularly aquatic—are scarce. Here, we tested the relative importance of species richness and cover of rooted aquatic vegetation, as well as cover of the loose-lying form of the macroalgae bladderwrack (Fucus vesiculosus), for ecosystem multifunctionality in shallow bays along the western Baltic Sea coast. We estimated multifunctionality based on four indicators of functions that support ecosystem services: recruitment of large predatory fish, grazer biomass, inverted ‘nuisance’ algal biomass, and water clarity. Piecewise path analysis showed that multifunctionality was driven by high cover of rooted aquatic vegetation and bladderwrack, particularly when the two co-occurred. This synergistic effect was nearly three times as strong as a negative effect of land-derived nitrogen loading. Species richness of aquatic vegetation indirectly benefitted multifunctionality by increasing vegetation cover. Meanwhile, high bladderwrack cover tended to decrease vegetation species richness, indicating that bladderwrack has both positive and negative effects on multifunctionality. We conclude that managing for dense and diverse vegetation assemblages may mitigate effects of anthropogenic pressures (for example, eutrophication) and support healthy coastal ecosystems that provide a range of benefits. To balance the exploitation of coastal ecosystems and maintain their multiple processes and services, management therefore needs to go beyond estimation of vegetation cover and consider the diversity and functional types of aquatic vegetation.
Graceful extensibility in asset management: extending the capacity to adapt in managing cyber-physical railway systems
Authors: Moerman Jan-jaap , Schraagen Jan Maarten , Braaksma Jan , van Dongen Leo 
Published: March 2021

AbstractGraceful extensibility has been recently introduced and can be defined as the ability of a system to extend its capacity to adapt when surprise events challenge its boundaries. It provides basic rules that govern adaptive systems. Railway transportation systems can be considered cyber-physical systems that comprise interacting digital, analog, physical, and human components engineered for safe and reliable railway transport. This enables autonomous driving, new functionalities to achieve higher capacity, greater safety, and real-time health monitoring. New rolling stock introductions require continuous adaptations to meet the challenges of these complex railway systems as an introduction takes several years to complete and deals with changing stakeholder demands, new technologies, and technical constraints which cannot be fully predicted in advance. To sustain adaptability when introducing new rolling stock, the theory of graceful extensibility might be valuable but needs further empirical testing to be useful in the field. This study contributes by assessing the proto-theorems of graceful extensibility in a case study in the railway industry by means of adopting pattern-matching analysis. The results of this study indicate that the majority of theoretical patterns postulated by the theory are corroborated by the data. Guidelines are proposed for further operationalization of the theory in the field. Furthermore, case results indicate the need to adopt management approaches that accept indeterminism as a complement to the prevailing deterministic perspective, to sustain adaptability and deal effectively with surprise events. As such, this study may serve other critical asset introductions dealing with cyber-physical systems in their push for sustained adaptability.
Pioneers of German-Polish inclusive exchange: Jaczewski’s and Kluge’s Europeanization in education despite the Iron Curtain
Authors: Toczyski Piotr , Broecher Joachim , Painter Janet 
Published: March 2021

AbstractHistorical and autobiographical approaches are combined with interviews to analyze the case of the Europa-Kontakt in pre-1989 Poland and West Germany within the framework of Europeanization. The international education encounters exemplify the tendencies to Europeanize, which emerged in both countries despite the Iron Curtain. The painful relationship between Poland and Germany is contrasted with the personal trust and cooperation between Polish and German exchange pioneers since the 1970s. Their pioneering work focused on multinational inclusion, participation, intercultural learning, gifted education, creativity, and building leadership skills. It merged German adaptation of the United States’ HighScope model with philosophy of encounters typical of scouting tradition, Janusz Korczak’s pedagogy, and Carl Rogers’ humanistic psychology, preparing ground for the 1989–2004 European Union enlargement process.
Creativity as a function of problem-solving expertise: posing new problems through investigations
Authors: Elgrably Haim , Leikin Roza 
Published: March 2021

AbstractThis study was inspired by the following question: how is mathematical creativity connected to different kinds of expertise in mathematics? Basing our work on arguments about the domain-specific nature of expertise and creativity, we looked at how participants from two groups with two different types of expertise performed in problem-posing-through-investigations (PPI) in a dynamic geometry environment (DGE). The first type of expertise—MO—involved being a candidate or a member of the Israeli International Mathematical Olympiad team. The second type—MM—was comprised of mathematics majors who excelled in university mathematics. We conducted individual interviews with eight MO participants who were asked to perform PPI in geometry, without previous experience in performing a task of this kind. Eleven MMs tackled the same PPI task during a mathematics test at the end of a 52-h course that integrated PPI. To characterize connections between creativity and expertise, we analyzed participants’ performance on the PPI tasks according to proof skills (i.e., auxiliary constructions, the complexity of posed tasks, and correctness of their proofs) and creativity components (i.e., fluency, flexibility and originality of the discovered properties). Our findings demonstrate significant differences between PPI by MO participants and by MM participants as reflected in the more creative performance and more successful proving processes demonstrated by MO participants. We argue that problem posing and problem solving are inseparable when MO experts are engaged in PPI.
Density Estimation of Nemopilema nomurai (Scyphozoa, Rhizostomeae) Using a Drone
Authors: Choi Seo Yeol , Kim Hyeon Jung , Seo Min Ho , Soh Ho Young 
Published: March 2021

AbstractResearch to understand the distribution and density of jellyfish is actively being conducted using training ships, but this is hindered by the high cost of manpower and the limitations of the irradiation area. Unmanned aerial vehicles (UAVs or drones), however, provide cost-effective means for assessing marine animal populations. Therefore, we tested the application of UAVs in estimating jellyfish density and probed the altitude-dependent suitability of these devices. We analyzed images obtained by a drone as well as by manual counting and used ImageJ to measure the density of Nemopilema nomurai off Sang-Chuja Island, Jeju, South Korea. Analysis of the image obtained at altitudes of 5–120 m allowed for the identification of 2–173 individuals, while 1.49–9.09 individuals were identified per 100 m2. Jellyfish density data measured by manual count and by ImageJ did not show any difference below 90 m; however, a difference was presented at altitudes of 100 m (98%) and 120 m (95%). These results demonstrate the potential of drones for jellyfish monitoring and recommend an optimal altitude for observation.
Preconception expanded carrier screening: a focus group study with relatives of mucopolysaccharidosis type III patients and the general population
Authors: Conijn Thirsa , van Dijke Ivy , Haverman Lotte , Lakeman Phillis , Wijburg Frits A , Henneman Lidewij 
Published: March 2021

AbstractPreconception expanded carrier screening (ECS) enables prospective parents to assess their risk of having a child with an autosomal recessive disorder. Knowledge on motivations, feelings, and considerations people have towards the offer and use of ECS is limited. To enrich the public and professional discussion on ECS implementation, this study explored the perspectives towards various aspects of ECS in seven focus groups compromising first- and second-degree relatives of MPS III patients (N=9, N=4, N=5, N=5) and members of the general Dutch population (N=6, N=7, N=5). The focus groups were audio recorded and the transcripts were qualitatively analyzed to identify themes. Both relatives of MPS III patients and participants from the general population supported offering ECS, in particular for severe, childhood-onset disorders. Important barriers identified for ECS were a lack of genetic knowledge and a perceived lack of personal relevance and awareness, as well as out-of-pocket costs of testing. The majority of participants would prefer full disclosure of individual test results instead of couple-based test results. Moreover, offering people a choice for the way of reporting was proposed. All participants agreed that more efforts, for example by governmental campaigns, should be made to increase awareness on the availability, potentials, and limitations of ECS. Educating prospective parents about ECS is essential for increasing awareness and informed decision making. This study provides valuable insights that can be used by governments and public health authorities when considering implementation of preconception ECS.
An Impulse-Regime Switching Game Model of Vertical Competition
Authors: Aïd René , Campi Luciano , Li Liangchen , Ludkovski Mike 
Published: March 2021

AbstractWe study a new kind of nonzero-sum stochastic differential game with mixed impulse/switching controls, motivated by strategic competition in commodity markets. A representative upstream firm produces a commodity that is used by a representative downstream firm to produce a final consumption good. Both firms can influence the price of the commodity. By shutting down or increasing generation capacities, the upstream firm influences the price with impulses. By switching (or not) to a substitute, the downstream firm influences the drift of the commodity price process. We study the resulting impulse-regime switching game between the two firms, focusing on explicit threshold-type equilibria. Remarkably, this class of games naturally gives rise to multiple potential Nash equilibria, which we obtain thanks to a verification-based approach. We exhibit three candidate types of equilibria depending on the ultimate number of switches by the downstream firm (zero, one or an infinite number of switches). We illustrate the diversification effect provided by vertical integration in the specific case of the crude oil market. Our analysis shows that the diversification gains strongly depend on the pass-through from the crude price to the gasoline price.
Classification of Wines Using Principal Component Analysis
Authors: Barth Jackson , Katumullage Duwani , Yang Chenyu , Cao Jing 
Published: March 2021

AbstractClassification of wines with a large number of correlated covariates may lead to classification results that are difficult to interpret. In this study, we use a publicly available dataset on wines from three known cultivars, where there are 13 highly correlated variables measuring chemical compounds of wines. The goal is to produce an efficient classifier with straightforward interpretation to shed light on the important features of wines in the classification. To achieve the goal, we incorporate principal component analysis (PCA) in the k-nearest neighbor (kNN) classification to deal with the serious multicollinearity among the explanatory variables. PCA can identify the underlying dominant features and provide a more succinct and straightforward summary over the correlated covariates. The study shows that kNN combined with PCA yields a much simpler and interpretable classifier that has comparable performance with kNN based on all the 13 variables. The appropriate number of principal components is chosen to strike a balance between predictive accuracy and simplicity of interpretation. Our final classifier is based on only two principal components, which can be interpreted as the strength of taste and level of alcohol and fermentation in wines, respectively. (JEL Classifications: C10, Cl4, D83)
Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function ofMegalobrama amblycephala
Authors: Xu Chao , Li Yuan-You , Brown Paul B. , Liu Wen-Bin , Gao Liu-Ling , Ding Zhi-Rong , Li Xiang-Fei , Xie Di-Zhi 
Published: March 2021

AbstractA12-week experiment was conducted to evaluate the influences of thiamine ongrowth performance, and intestinal mitochondrial biogenesis and function of Megalobramaamblycephala fed a high-carbohydrate (HC) diet. Fish (24·73 (sem0·45) g) were randomly assigned to one of four diets: two carbohydrate (CHO) levels (30 and 45 %) and two thiamine levels (0 and 1·5 mg/kg). HC diets significantly decreased DGC, GRMBW, FIMBW, intestinal activities of amylase, lipase, Na+, K+-ATPase, CK, complexes I, III and IV, intestinal ML, number of mitochondrial per field, ΔΨm, the P-AMPK: T-AMPK ratio, PGC-1βprotein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, mitochondrial transcription factor A, Opa-1, ND-1 and COX-1 and 2, while the opposite was true for ATP, AMP and reactive oxygen species, and the transcriptions of dynamin-related protein-1, fission-1 and mitochondrial fission factor. Dietarythiamine concentrations significantly increased DGC, GRMBW, intestinal activities of amylase, Na+, K+-ATPase, CK, complexes I and IV, intestinal ML, number of mitochondrial per field, ΔΨm, the P-AMPK:T-AMPK ratio, PGC-1βprotein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, ND-1, COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between dietary CHO and thiamine was observed in DGC, GRMBW, intestinal activities of amylase, CK, complexes I and IV, ΔΨm, the AMP:ATP ratio, the P-AMPK:T-AMPK ratio, PGC-1βprotein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation improved growth performance, and intestinal mitochondrial biogenesis and function ofM. amblycephalafed HC diets.