bluuebunny/crossref_metadata_2025_split huggingface dataset:
The novel role of circular RNA ST3GAL6 on blocking gastric cancer malignant behaviors through autophagy regulated by the FOXP2/MET/mTOR axis
Authors: Xu Penghui , Zhang Xing , Cao Jiacheng , Yang Jing , Chen Zetian , Wang Weizhi , Wang Sen , Zhang Lu , Xie Li , Fang Lang , Xia Yiwen , Xuan Zhe , Lv Jialun , Xu Zekuan 
Published: August 2021

Abstract
Gastric cancer (GC) ranks third in motality among all cancers worldwide. Circular RNAs (circRNAs) play essential roles in the malignant progression and metastasis of gastric cancer. As a transcription factor, FOXP2 is involved in the progression of many tumours. However, the regulation and association between circRNAs and FOXP2 remain to be discovered. In our study, CircST3GAL6 was significantly depressed in GC tissues and cells. circST3GAL6 overexpression inhibited the proliferation, invasion and metastasis of GC cells in vitro and in vivo. Importantly, circST3GAL6 overexpression induced apoptosis and promote autophagy in GC cells. Furthermore, we found that circST3GAL6 sponged miR-300 and subsequently regulated FOXP2. We further revealed that FOXP2 suppressed the activation of the Met/AKT/mTOR axis, a classic pathway that regulates autophagy-mediated proliferation and migration. In summary, our ﬁndings revealed that circST3GAL6 functions as a tumour suppressor through the miR-300/FOXP2 axis in GC, regulates apoptosis and autophagy through FOXP2-mediated transcriptional inhibition of the MET axis and may be a biomarker for GC treatment.
Disruption of Normal Refractive Development in Individuals with Ocular and Oculocutaneous Albinism
Authors: Verkicharla Pavan Kumar , Thakur Swapnil , Kammari Priyanka , Dhakal Rohit , Das Anthony Vipin 
Published: August 2021

Abstract
PurposeAlbinism is known to disrupt emmetropisation in animal models. However, it is not clear if the same effect is seen in humans. This study aimed to investigate the refractive profile in individual diagnosed with ocular albinism (OA) and oculocutaneous albinism (OCA) based on a large dataset.MethodsRequired data from 618 individuals (61% males and 39% females) diagnosed with albinism were exported from the eyeSmart electronic medical records of L V Prasad Eye Institute. Overall, there were 112 (18%) individuals diagnosed with OA and 506 (82%) with OCA. Based on the spherical equivalent refraction (SER), individuals were classified as emmetropes, myopes, and hyperopes.ResultsThe overall spherical equivalent refraction of the individuals ranged from -25.00D to +12.00D with median 0.25 (-2.00 to 2.25). The proportion of individuals with albinism (combined OA, OCA) having hyperopia and myopia (overall: N=282;46% vs. N=245;40%) were similar (p = 0.18), and the least were with emmetropia (overall: N=91;14%). Across all the age groups (0-10, 11-20, 21-30, > 30 years) the frequency of hyperopes and myopes were significantly higher (p<0.05) compared to emmetropes. Both high degrees of hyperopia and myopia were found in individual diagnosed with OA and OCA. Irrespective of the albinism type, with-the-rule (70%) astigmatism was the most prevalent compared to other types of astigmatism. The frequency of with-the-rule astigmatism was significantly high in the presence of nystagmus compared to individuals with no nystagmus in both OA (75 % vs 25%, p = 0.01) and OCA (77% vs 23 %, p = 0.014) group. Conclusion The presence of both high hyperopia and high myopia and very less numbers with emmetropia across all age groups indicates disrupted normal refractive development in individuals with albinism. With-the-rule astigmatism and nystagmus may result in meridional degradation of retinal image leading to impairment of normal emmetropisation process in individuals with albinism.
Transcriptome Analysis of Related-genes to MeJA Treatment Metabolite Synthesis in Hairy Roots of Broccoli (Brassica oleracea L. var. Italic Planch)
Authors: Bao jinyu , Lu Xu , Ma Lei , Zhang Xiumin , Tian Peng , Zhang Xiaoling , Li Sheng , Ma Shaoying , Yang Jie , Lu Yaqi , Wei Yunchun , Zhang Congcong 
Published: August 2021

Abstract
Hairy roots obtained by infecting broccoli (Brassica oleracea L. var. Italic Planch) leaves with Agrobacterium rhizogenes (ATCC15834) had the characteristics of phytohormone autonomy, genetic stability and can produce a large amount of anti-cancer substance Sulforaphane (SF) and SF biosynthetic precursors substance Glucoraphanin (GRA). The production of SF in hairy roots of broccoli increased significantly under the induction of exogenous signal molecule Methyl jasmonate (MeJA). However, the molecular mechanism of MeJA treatment hairy roots of broccoli have not been reported. In this study, the optimal concentration of MeJA for having treatment broccoli hairy roots were selected based on the yield of GRA and SF. After grew 18 days, broccoli hairy roots were treated with 10 mmol/L MeJA for 0, 3, 6, 9 and 12 h respectively. Compared with 0 h, the yields of GRA and SF increased under other treatments. The highest yield of GRA and SF was noted at 9 h, which were 2.22-fold and 1.74-fold of 0 h, respectively. Broccoli (Brassica oleracea L. var. botrytis L) as reference genome, and 1195 co-differentially expressed genes (DEGs) at 0, 3, 6, 9 and 12 h was observed under treatment 10 mmol/L MeJA, which there were 3,826 down-regulated and 574 up-regulated genes. The six key genes that regulated Glucosinolates (GLS) synthesis, MAM1, CYP79B1, CYP83B1, UGT74B1, and FMOGS-OX5, were up-regulated at 0 and 3 h, and down-regulated at the rest of the time; BCAT2 was up-regulated at 6, 9, 12 h, and at 0, 3 h expression was down-regulated, transcription factors MYB34 and MYB122 were up-regulated at 3 h, and down-regulated at other time points, MYB51 was up-regulated at 0, 3 h and down-regulated at 6, 9, 12 h. The pathway diagram of GRA biosynthesis and transformation pathway in broccoli hairy roots treatment by MeJA simulated, and the molecular mechanis0m of GRA biosynthesis and SF accumulation in broccoli hairy roots under MeJA treatment were revealed.
Community Violence and Internalizing Mental Health Symptoms in Adolescents: Systematic Review.
Authors: Miliauskas Claudia Reis , Faus Daniela Porto , Cruz Valéria Lima da , Vallaperde João Gabriel Rega do Nascimento , Junger Washington , Lopes Claudia Souza 
Published: August 2021

Abstract
Purposes: Mental health diseases (MHD) are responsible for 16% of the global burden of disease in adolescents. This review focus on one contextual factor nominated community violence (CV) that can contribute to the development of MHD. Objective: to evaluate the impact of CV on internalizing mental health symptoms (IMHS) in adolescents, to investigate whether different proximity to CV (witness x victim) is associated with different risks and to identify whether gender, age, and race moderate this association. Methods: systematic review of observational studies. Population includes adolescents (10 - 24 years), exposition involves individuals exposed to CV and outcomes consists of IMHS. Selection, extraction and quality assessment were performed independently by two research. Results: 2987 works were identified, after selection and extraction it remained 42. Higher exposure to CV were associated positively with IMHS. Being a witnessing is less harmful for mental health than being a victim. Age and race did not appear in the results as modifiers, but masculine gender and family support appear as a protective factor in some studies. Conclusion: This review confirms the positive relationship between CV and IMHS in adolescents and brings relevant information that can direct public efforts to build policies in prevention of both problems.
Integrated Secure Messaging to Enhance Medical Education: A Mixed Methods Study
Authors: Nichols Laura , Guerrero Dubert , Mannuru Devendranath , Basson Marc D. , Sahmoun Abe E. , Bande Dinesh 
Published: August 2021

Abstract
BackgroundInstant messaging applications and texting are useful for educating and communicating with medical students; however, they present patient privacy concerns and do not address the challenge of student inclusion in patient care communication. EMR-integrated secure messaging offers an opportunity to include students on team communication, enhance their medical education, and ensure patient privacy. Methods Between July 2019 through March 2020, we performed a mixed method study to evaluate use of EPIC® Secure Chat as a means of enhancing student education and team communication. We promoted use of secure messaging in orientation, performed a pre- and post-rotation survey to assess perceptions of secure chat effect on communication, and directly reviewed and categorized messages. ResultsTwenty-four 3rd and 4th year students completed the pre-rotation survey, and 22 completed the post-rotation survey. Twelve (50%) students reported the quality of communication with faculty was either good or very good prior to internal medicine rotation, while 20 (91%) reported this post-rotation (p-value 0.001). There was a similar improvement in communication with ancillary staff. Nineteen (86%) students felt that secure messaging improved their communication with faculty. On message review, threads were frequently logistical, but also often included discussions of patient management. ConclusionsStudents viewed Secure Chat as having a favorable effect on their communication with team members and reported communication on internal medicine to be improved compared to prior rotations. Messages included students on important patient care conversations. Secure messaging offers a novel medium to improve team communication, enhance student education and maintain patient privacy.
A Systematic Review of the Effects of Psychiatric Medications on Social Cognition
Authors: Haime Zoë , Watson Andrew J , Crellin Nadia , Marston Louise , Joyce Eileen , Moncrieff Joanna 
Published: August 2021

Abstract
IntroductionSocial cognition is an important area of mental functioning relevant to psychiatric disorders and social functioning, that may be affected by psychiatric drug treatments. The aim of this review was to investigate the effects of medications with sedative properties, on social cognition. MethodThis systematic review included experimental and neuroimaging studies investigating drug effects on social cognition. Data quality was assessed using a modified Downs and Black checklist [40]. The review used narrative synthesis to analyse the data.Results40 papers were identified for inclusion, 11 papers investigating benzodiazepine effects, and 29 investigating antipsychotic effects, on social cognition.Narrative synthesis showed that diazepam impairs healthy volunteer’s emotion recognition, with supporting neuroimaging studies showing benzodiazepines attenuate amygdala activity. Studies of antipsychotic effects on social cognition gave variable results. However, many of these studies were in patients already taking medication, and potential practice effects were identified due to short-term follow-ups.ConclusionHealthy volunteer studies suggest that diazepam reduces emotional processing ability. The effects of benzodiazepines on other aspects of social cognition, as well as the effects of antipsychotics, remain unclear. Interpretations of the papers in this review were limited by variability in measures, small sample sizes, and lack of randomisation. More robust studies are necessary to evaluate the impact of these medications on social cognition.
Global Assessment of Climatic Niche Shifts in Three Rumex Species
Authors: Carlin Thomas , Bufford Jennifer , Hulme Philip , Godsoe William 
Published: August 2021

Abstract
Climatic niche shifts occur when species occupy different climates in the introduced range than in their native range. We know that climatic niche shifts are common occurrences, however we do not currently understand whether climatic niche shifts can consistently be predicted across the globe. Using three congeneric weed species, we investigate whether the known presence of a climatic niche shift in one range can help predict a species’ distribution in other ranges. We consider whether data either from other ranges or from closely related species can help predict whether climatic niche shifts will occur. We compared the climatic conditions occupied by Rumex obtusifolius, R. crispus, and R. conglomeratus between their native range (Eurasia) and three different introduced ranges (North America, Australia, New Zealand). We consider metrics of niche overlap, expansion, unfilling, pioneering, and similarity to determine whether i) climatic niche shifts have occurred and ii) climatic niche shifts were consistent across ranges and congeners. We found that the presence and direction of climatic niche shifts is inconsistent across ranges for all three species. Within an introduced range, however, niche shifts were similar between species. Despite this, species distributions outside of their native range could not be reliably predicted by the distributions of congeners in either their native or introduced ranges. This study is the first of its kind to consider niche shifts across multiple introduced ranges and species, highlighting new challenges in predicting species distributions when species undergo climatic niche shifts.
Investigating Fish Contamination Scenario and Community Willingness to Adopt Consumption Advice Proposing an Advisory Model
Authors: Alam Lubna , Sumaila Ussif Rashid , Bari Md Azizul , Rusydy Ibnu , Musthafa Mohamed Saiyad , Mokhtar Mazlin 
Published: August 2021

Abstract
Consumption advice to ensure the health and safety of fish consumers remains urgent to handle the ever-increasing panic over heavy metal toxicity. Moreover, studies of fish consumption rarely focus on the perceptions and awareness of consumers. Considering this, the present study examines the knowledge and perceptions of the risks and benefits of fish consumption among consumers of Laguna de Bay to explore their willingness to follow fish consumption advice. The seasonal variation in selected types of heavy metal contamination in two commercially important fish species, tilapia and mudfish, was analysed and a vulnerability map based on the risk‒benefit ratio was produced for Laguna de Bay. Furthermore, this study formulates fish consumption advice for consumers of fish in the area. Primary data on consumers’ perceptions were collected through a questionnaire, whereas heavy metal contamination data were compiled from the best available literature. We concluded that people’s willingness to adopt consumption advice is mostly dependent on their existing level of fish consumption. Moreover, consumption advice is formulated to indicate restrictions on consumption for the areas identified as vulnerable due to contamination. This empirical study can serve as a model for the future development of fish consumption advice in the region.
Immobilization of Eversa Lipases on Hydrophobic Supports for Production of Oleate Esters Solvent-Free
Authors: Fernandez-Lorente Gloria , Remonatto Daniela , Oliveira J. Vladimir , Guisan J. Manuel , Oliveira Débora , Ninow Jorge 
Published: August 2021

Abstract
Lipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the lipases Eversa showed high catalytic activity. However, the more stable and efficient derivative was Eversa® Transform immobilized on Sepabeads C-18. A 98 wt% of ethyl ester of fatty acid (FAEE) was obtained, in 3 hours at 40ºC, ethanol/sunflower oil molar ratio of 3:1 and a 10 wt% of the immobilized biocatalyst. After 6 reaction cycles, the immobilized biocatalyst preserved 70 wt% of activity. Both lipases immobilized in Sepabeads C-18 were highly active and stable in the presence of ethanol. The immobilization of Eversa Transform and Eversa Transform 2.0 in hydrophobic supports described in this study appears to be a promising alternative to the immobilization and application of these news lipases still unexplored.
Heavy Metals In Surface Sediments Of The Intertidal Thai Binh Coast, Gulf of Tonkin, East Sea, Vietnam: Distribution, Accumulation, And Contamination Assessment
Authors: Duong Lim Thi , Nguyen Bac Quang , Dao Cham Dinh , Dao Nhiem Ngoc , Nguyen Huong Thi Lan , Nguyen Thuy Thi Huong , Nguyen Chi Thi Ha , Duong Dien Cong , Pham Ngo Nghia 
Published: August 2021

Abstract
Heavy metals contamination in sediments may endanger ecosystems and human health via the food chain. In fact, there is little to no understanding about heavy metal accumulation in surface sediment of one of the most economically important marine bodies for Vietnam, the Thai Binh Coast, where five large rivers co-discharge into the Gulf of Tonkin. Twenty-seven surface sediment samples were collected from the intertidal regions and analyzed for: arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), and zinc (Zn) using inductively coupled plasma mass spectroscopy (ICP-MS). The studied area exhibited a large spatial variation in the concentration of heavy metals, e.g., the dry sediment concentration of Cd was the least (0.05–0.49 mg.kg−1), whereas that of Zn was the greatest (45.4–252 mg.kg−1). Based on the geoaccumulation index (Igeo), most of the studied heavy metals were accumulated at low pollution levels, except four locations exhibited moderately and highly polluted levels of Hg with Igeo Hg values from 1.92 to 2.66. Whereas the high contamination factor value implicated that not only Hg but also all other detected heavy metals in this area resulted from anthropogenic activities along the coast and the river upstream. This implied the need for quick action from the government.
Non-Compliance To Social Distancing During COVID-19 Pandemic: A Comparative Cross-Sectional Study Between the Developed and Developing Countries
Authors: Aamir Faiqa Binte , Zaidi Syeda Maria Ahmad , Abbas Saadia , Aamir Syed Roohan , Zaidi Syed Nauman Ahmad , Lal Karishma Kanhya , Fatima Syeda Sadia 
Published: August 2021

Abstract
Background:

As of June 19th, 2021, there have been 178,765,626 reported COVID-19 cases and confirmed 3,869,994 COVID-19 related deaths. Despite the mass vaccination campaigns, number of SARS-CoV-2 infection cases continue to rise. Therefore, it’s important to control its spread for which the most potent method remains social distancing. Due to the diversity in the severity of the effect of the pandemic on different countries, it’s imperative to study the social distancing behavior among population in the developed and developing countries where the factors such as socioeconomic status, education, overpopulation, religious beliefs, and misconceptions play a role in altering the population’s behavior.
Methods:

In this cross-sectional study, a total of 384 participants from 14 different countries were surveyed via an online REDCap form.
Results:

In this study it was highlighted that although the knowledge regards to COVID-19 pandemic and its related prevention remains high, the overall compliances in both the developed and developing countries remain poor, the lowest being in the senior age group (≥ 65 years). It was found that out of all the age groups, adults aged between 25–64 years were the most compliant to social distancing. (p value = 0.003) Population from the developing countries were more compliant to all preventative measures against COVID-19 spread except for in handwashing compliance where the difference between the two populations remains insignificant. (p value = 0.038, < 0.001, 0.016) Socioeconomic status, prior history of COVID-19 infection or presence of comorbidities did not significantly affect compliance rates however, participants with no prior history of this infection were found to be more compliant to donning a mask in public as compared to those with a positive history of SARS-CoV-2 infection. (p value = 0.044) Additionally, participants with no family history for comorbidities in developing countries had a higher hand washing and mask compliance as compared to those who had a positive family history. (p value = 0.035)
Conclusion:

Mass campaigns about awareness related to the preventative measures against COVID-19 remain essential in controlling the disease spread as despite having an overall high COVID-19 related literacy, compliance remains subpar in both developing and developed countries.
Availability of Post-Abortion Family Planning Services and Contraception in Hunan Province, China：A Cross-Sectional Study
Authors: Tong Chen-xi , Meng Yan-Ting , Yue Jing , Fan Ling , Nie Min , Li Ting , Luo Yang 
Published: August 2021

Abstract
Background

Although the project of post-abortion family planning services (PAFPS) has been launched in China for many years, it remains to know how many people received it after induced abortions (IAs), and the prevalence of repeated IAs is still high, it may be related to the quality of PAFPS. Therefore, this study aimed to understand the prevalence of PAFPS and explore the factors related to contraceptive method choice after receiving PAFPS in Hunan Province, China.
Methods

This study was a secondary data analysis from the Women Health Needs Survey (WHNS). Women aged 20–49 with induced abortion history and those who had received PAFPS were selected. Chi-square test and multiple logistic regression analyses were used to identify the related factors about the use of contraceptive methods after receiving PAFPS.
Results

The prevalence of PAFPS was 84.7% in Hunan, China. Women were more likely to use unreliable contraceptive methods if they experienced painless IA (ORs = 3.353, 95% CIs: 1.151–9.769). Farmers or workers (OR = 0.297, 95% CIs: 0.130–0.678), those were followed up after IA (ORs = 0.506, 95% CIs: 0.303–0.846) and those acquired reliable recommendations (ORs = 0.109, 95% CIs: 0.044–0.274) were significantly less likely to use unreliable contraceptive methods.
Conclusion

This study is the first to determine the availability of PAFPS from receivers' perspectives and based on a community population to assess the relationship between PAFPS and contraception in China. PAFPS providers' advice and follow-up play a vital role in women's choice of contraceptive methods. However, the prevalence of follow-up was a little low, and it needed to develop more professional staff in PAFPS. In addition, more research is necessary on how to improve the quality of contraceptive counseling.
Trial registration:
Not applicable