bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pro‐vaccination subjective norms moderate the relationship between conspiracy mentality and vaccination intentions
Authors: Winter Kevin , Pummerer Lotte , Hornsey Matthew J. , Sassenberg Kai 
Published: July 2021

ObjectivesIncreasing vaccination hesitancy threatens societies’ capacity to contain pandemics and other diseases. One factor that is positively associated with vaccination intentions is a supportive subjective norm (i.e., the perception that close others approve of vaccination). On the downside, there is evidence that negative attitudes toward vaccinations are partly rooted in conspiracy mentality (i.e., the tendency to believe in conspiracies). The objective of this study is to examine the role of subjective norms in moderating the association between conspiracy mentality and vaccine hesitancy. We examined two competing predictions: Are those high in conspiracy mentality immune to subjective norms, or do subjective norms moderate the relationship between conspiracy mentality and vaccination intentions?MethodsWe conducted five studies (total N = 1,280) to test these hypotheses across several vaccination contexts (some real, some fictitious). We measured conspiracy mentality, vaccination intentions, subjective norms, attitudes toward vaccination, and perceived behavioural control.ResultsA merged analysis across the studies revealed an interaction effect of conspiracy mentality and subjective norm on vaccination intentions. When subjective norm was high (i.e., when participants perceived that close others approved of vaccines) conspiracy mentality no longer predicted vaccination intentions. This was consistent with the moderating hypothesis of subjective norms and inconsistent with the immunity hypothesis.ConclusionsThe typical negative relationship between conspiracy mentality and vaccination intentions is eliminated among those who perceive pro‐vaccination subjective norms. Although correlational, these data raise the possibility that pro‐vaccination views of friends and family can be leveraged to reduce vaccine hesitancy.
Glycocalyx is critical for blood‐brain barrier integrity by suppressing caveolin1‐dependent endothelial transcytosis following ischemic stroke
Authors: Zhu Juan , Li Zheqi , Ji Zhong , Wu Yongming , He Yihua , Liu Kewei , Chang Yuan , Peng Yuqin , Lin Zhenzhou , Wang Shengnan , Wang Dongmei , Huang Kaibin , Pan Suyue 
Published: July 2021

AbstractThe breakdown of the blood‐brain barrier (BBB) is related to the occurrence and deterioration of neurological dysfunction in ischemic stroke, which leads to the extravasation of blood‐borne substances, resulting in vasogenic edema and increased mortality. However, a limited understanding of the molecular mechanisms that control the restrictive properties of the BBB hinders the manipulation of the BBB in disease and treatment. Here, we found that the glycocalyx (GCX) is a critical factor in the regulation of brain endothelial barrier integrity. First, endothelial GCX displayed a biphasic change pattern, of which the timescale matched well with the biphasic evolution of BBB permeability to tracers within the first week after t‐MCAO. Moreover, GCX destruction with hyaluronidase increased BBB permeability in healthy mice and aggravated BBB leakage in transient middle cerebral artery occlusion (t‐MCAO) mice. Surprisingly, ultrastructural observation showed that GCX destruction was accompanied by increased endothelial transcytosis at the ischemic BBB, while the tight junctions remained morphologically and functionally intact. Knockdown of caveolin1 (Cav1) suppressed endothelial transcytosis, leading to reduced BBB permeability, and brain edema. Lastly, a coimmunoprecipitation assay showed that GCX degradation enhanced the interaction between syndecan1 and Src by promoting the binding of phosphorylated syndecan1 to the Src SH2 domain, which led to rapid modulation of cytoskeletal proteins to promote caveolae‐mediated endocytosis. Overall, these findings demonstrate that the dynamic degradation and reconstruction of GCX may account for the biphasic changes in BBB permeability in ischemic stroke, and reveal an essential role of GCX in suppressing transcellular transport in brain endothelial cells to maintain BBB integrity. Targeting GCX may provide a novel strategy for managing BBB dysfunction and central nervous system drug delivery.
The Rosebank Field, NE Atlantic: Volcanic characterisation of an inter‐lava hydrocarbon discovery
Authors: Millett John M. , Jerram Dougal A. , Manton Ben , Planke Sverre , Ablard Peter , Wallis Dirk , Hole Malcolm J. , Brandsen Harry , Jolley David W. , Dennehy Cliona 
Published: July 2021

AbstractThe Rosebank Field is located in the Faroe‐Shetland Basin and hosts hydrocarbons within siliciclastic sediments interlayered with volcanic packages of the Late Paleocene to Early Eocene aged Flett Formation. Within this study the volcanic sequences are investigated based on an integrated appraisal of available drill cuttings, sidewall cores, core and wireline logs including image log and geochemical logs from eight wells supported by 3D seismic data. The Rosebank lower (RLV), middle (RMV) and upper (RUV) volcanic sequences are inter‐layered with Colsay Member (C1–C4) fluvial to shallow marine siliciclastic intervals. A comprehensive cross‐field borehole based lithofacies interpretation is presented characterising simple, compound and ponded effusive lava flow facies along with pillow lavas, invasive lava flows, volcaniclastic sediments and complex lava–sediment interactions. Geochemical analyses of core, sidewall core, and hand‐picked cuttings spanning the field reveal separate high‐titanium (RHT) and relatively lower‐titanium (RLT) basaltic magma suites. These compositions can be identified and correlated across much of the field utilising geochemical logging data which, in combination with the geochemical analyses, reveals a two‐part stratigraphic sub‐division of each of the RLV, RMV and RUV. Geochemical logging data is also used to define a volcanic proxy (Fe/10+Ti) which utilises the elevated iron (Fe) and titanium (Ti) within all effusive and volcaniclastic basaltic lithologies to differentiate siliciclastic from volcaniclastic sediments where other logging parameters overlap. By comparing the borehole analyses with seismic data, a localised eruptive vent is interpreted within the north of the field. Finally, a cross‐field volcanic model is presented and compared with relevant global field analogues, providing a constrained spatial framework for sub‐surface modelling of inter‐volcanic sequences.
Reactive oxygen species in cancer: Current findings and future directions
Authors: Nakamura Hajime , Takada Kohichi 
Published: July 2021

AbstractReactive oxygen species (ROS), a class of highly bioactive molecules, have been widely studied in various types of cancers. ROS are considered to be normal byproducts of numerous cellular processes. Typically, cancer cells exhibit higher basal levels of ROS compared with normal cells as a result of an imbalance between oxidants and antioxidants. ROS have a dual role in cell metabolism: At low to moderate levels, ROS act as signal transducers to activate cell proliferation, migration, invasion, and angiogenesis. In contrast, high levels of ROS cause damage to proteins, nucleic acids, lipids, membranes, and organelles, leading to cell death. Extensive studies have revealed that anticancer therapies that manipulate ROS levels, including immunotherapies, show promising in vitro as well as in vivo results. In this review, we summarize molecular mechanisms and oncogenic functions that modulate ROS levels and are useful for the development of cancer therapeutic strategies. This review also provides insights into the future development of effective agents that regulate the redox system for cancer treatment.
Patterns of morphological simplification and innovation in the megadiverse Holometabola (Insecta)
Authors: Beutel Rolf Georg , Friedrich Frank , Economo Evan P. 
Published: July 2021

AbstractWe analyzed patterns of complexity and simplicity in holometabolan insects using parsimony and maximum‐likelihood. By contrast with other groups of arthropods (and most other groups of animals), insects have undergone a stepwise process of structural simplification in their evolution. The megadiverse Holometabola are characterized mainly by structurally simplified larvae, which differ strongly from the adults in their morphology and usually also in their life habits. Although smaller groups such as Neuropterida have largely maintained their structural complexity in adults and immature life stages, a series of reductions occurred with the appearance and diversification of Coleopterida, Mecopterida and especially Antliophora. Parasitic Strepsiptera or fleas display conspicuous patterns of reduction in different life stages and body regions, and high degrees of simplification also occur in groups with short‐lived adults. Larvae living in moist substrates display far‐reaching structural simplifications and also morphological uniformity, especially in the species‐rich Diptera, but also in other groups. Liquid feeding leads to correlated simplifications and innovation of adult head structures, especially of the mouthparts. Functional or anatomical dipterism leads to an optimization of the flight apparatus in most holometabolous groups, which is correlated with reductions in one of the pterothoracic segments, and coupled (e.g. by hamuli), partly reduced or transformed wings (e.g. halteres). In flightless groups, the pterothoracic skeleto‐muscular apparatus is strongly simplified. In the abdomen of adult females a stepwise reduction of the lepismatoid ovipositor occurs. By contrast, the male genital apparatus often undergoes an extreme diversification. Our evaluations revealed a highly correlated complexity between larval and adult stages.
Understanding mechanisms behind unwanted health behaviours in Nordic and South Asian women and how they affect their gestational diabetes follow‐ups: A qualitative study
Authors: Sharma Archana , Birkeland Kåre I. , Nermoen Ingrid , Qvigstad Elisabeth , Tran Anh T. , Gulseth Hanne L. , Sollid Stina T. , Wium Cecilie , Varsi Cecilie 
Published: July 2021

AbstractAimsThe type 2 diabetes risk following gestational diabetes mellitus (GDM) is high, particularly among South Asian women in Western countries. Our study aimed to advance the knowledge regarding the mechanisms behind suboptimal follow‐up in the Nordic and South Asian women with previous GDM by comparing (1) their experiences, (2) health and disease perceptions and (3) barriers to and facilitators of health‐promoting behaviours.MethodsThis qualitative study was conducted in three hospital outpatient clinics in Norway, comprising six focus group interviews with 28 women 1–3 years after a pregnancy with GDM. The participants were purposively sampled and grouped according to their ethnicity. The data were analysed using thematic analysis, and a theoretical approach was applied to support the analysis and discuss the study's findings.ResultsFive main themes were identified: lack of resilience, emotional distress, ‘caught between a rock and a hard place’, postpartum abandonment and insufficient guidance. The key determinants of the maintenance of unwanted health behaviours after GDM were consistent across the ethnic groups. Although the importance of a culturally sensitive approach was emphasised, it appeared secondary to the need for a more organised public healthcare during and after GDM.ConclusionsWomen's real‐life constraints, combined with the inadequate healthcare‐service implementation, could explain the non‐adherence to the lifestyle‐changes guidelines essential for preventing diabetes post‐GDM. We suggest promoting specific coping strategies and changing the healthcare service approach rather than relying on women's capacity to initiate the necessary changes.
Density influences the heritability and genetic correlations of fish behaviour under trawling‐associated selection
Authors: Crespel Amélie , Miller Toby , Rácz Anita , Parsons Kevin , Lindström Jan , Killen Shaun 
Published: July 2021

AbstractFishing‐associated selection is one of the most important human‐induced evolutionary pressures for natural populations. However, it is unclear whether fishing leads to heritable phenotypic changes in the targeted populations, as the heritability and genetic correlations of traits potentially under selection have received little attention. In addition, phenotypic changes could arise from fishing‐associated environmental effects, such as reductions in population density. Using fish reared at baseline and reduced group density and repeatedly harvested by simulated trawling, we show that trawling can induce direct selection on fish social behaviour. As sociability has significant heritability and is also genetically correlated with activity and exploration, trawling has the potential to induce both direct selection and indirect selection on a variety of fish behaviours, potentially leading to evolution over time. However, while trawling selection was consistent between density conditions, the heritability and genetic correlations of behaviours changed according to the population density. Fishing‐associated environmental effects can thus modify the evolutionary potential of fish behaviour, revealing the need to use a more integrative approach to address the evolutionary consequences of fishing.
A guide to the Michaelis–Menten equation: steady state and beyond
Authors: Srinivasan Bharath 
Published: July 2021

The modern definition of enzymology is synonymous with the Michaelis–Menten equation instituted by Leonor Michaelis and Maud Menten. Most textbooks, or chapters within, discussing enzymology start with the derivation of the equation under the assumption of rapid equilibrium (as done by Michaelis–Menten) or steady state (as modified by Briggs and Haldane) conditions to highlight the importance of this equation as the bedrock on which interpretation of enzyme kinetic results is dependent. However, few textbooks or monographs take the effort of placing the equation within its right historical context and discuss the assumptions that have gone into its institution. This guide will dwell on these in substantial detail. Further, this guide will attempt to instil a sense of appreciation for the mathematical curve rectangular hyperbola, its unique attributes and how ubiquitous the curve is in biological systems. To conclude, this guide will discuss the limitations of the equation, and the method it embodies, and trace the journey of how investigators are attempting to move beyond the steady‐state approach and the Michaelis–Menten equation into full progress curve, pre–steady state and single‐turnover kinetic analysis to obtain greater insights into enzyme kinetics and catalysis.
Cave amphipods reveal co‐variation between morphology and trophic niche in a low‐productivity environment
Authors: Premate Ester , Borko Špela , Delić Teo , Malard Florian , Simon Laurent , Fišer Cene 
Published: July 2021

Abstract

Identifying the relationships between morphology and trophic niche is at the core of functional morphology. Low resource diversity and fluxes of organic carbon are expected to constrain trophic specialisation of morphological structures because food resources are too scarce to promote trophic differentiation. However, species from low‐productivity habitats often exhibit specialised biological traits such as resistance to starvation and high food‐finding abilities, which may in turn release constraints on trophic and morphological differentiation among species.

Groundwaters are food resource‐limited because of the lack of photosynthetic production and limited inputs of organic carbon from surface ecosystems. We tested for co‐variation between morphology and trophic habits in co‐occurring Niphargus amphipods from five groundwater caves of the Dinaric Karst, Europe. We predicted that the size of gnathopods—the accessory feeding appendages—would positively co‐vary with trophic position: species with larger gnathopods should more easily grab and immobilise prey.

We quantified gnathopod size and shape by means of morphometric measurements and assessed isotopic niche, trophic position, and carbon signatures using nitrogen (δ15N) and carbon (δ13C) stable isotopes. We tested for correlation between morphological traits and trophic position and δ13C signatures while accounting for phylogenetic relationships among species.

All co‐occurring species differed morphologically in at least one gnathopod measurement and all of them differed in their isotopic niches. As predicted, gnathopod size increased with the increasing trophic position. This co‐variation probably reflects differences in detritivorous and predatory habits among species: amphipods with larger gnathopods, hence larger muscle and more powerful grip, could more easily subdue prey. Moreover, we found a significant correlation between gnathopod shape and the normalised δ13C values, indicating that shape of the gnathopods may be related to exploitation of different food resources.

We show that low‐productivity subterranean habitat species can exhibit strong trophic specialisation of morphological structures. Gnathopod size and shape of Niphargus amphipods are functional traits that co‐vary with trophic habits. Our findings pave the way for investigating how co‐variation of morphological and trophic traits may control energy flow and species’ coexistence at lower bounds of habitat productivity.
Major loss of coralline algal diversity in response to ocean acidification
Authors: Peña Viviana , Harvey Ben P. , Agostini Sylvain , Porzio Lucia , Milazzo Marco , Horta Paulo , Le Gall Line , Hall‐Spencer Jason M. 
Published: July 2021

AbstractCalcified coralline algae are ecologically important in rocky habitats in the marine photic zone worldwide and there is growing concern that ocean acidification will severely impact them. Laboratory studies of these algae in simulated ocean acidification conditions have revealed wide variability in growth, photosynthesis and calcification responses, making it difficult to assess their future biodiversity, abundance and contribution to ecosystem function. Here, we apply molecular systematic tools to assess the impact of natural gradients in seawater carbonate chemistry on the biodiversity of coralline algae in the Mediterranean and the NW Pacific, link this to their evolutionary history and evaluate their potential future biodiversity and abundance. We found a decrease in the taxonomic diversity of coralline algae with increasing acidification with more than half of the species lost in high pCO2 conditions. Sporolithales is the oldest order (Lower Cretaceous) and diversified when ocean chemistry favoured low Mg calcite deposition; it is less diverse today and was the most sensitive to ocean acidification. Corallinales were also reduced in cover and diversity but several species survived at high pCO2; it is the most recent order of coralline algae and originated when ocean chemistry favoured aragonite and high Mg calcite deposition. The sharp decline in cover and thickness of coralline algal carbonate deposits at high pCO2 highlighted their lower fitness in response to ocean acidification. Reductions in CO2 emissions are needed to limit the risk of losing coralline algal diversity.
Place matters: Analyzing the roots of political distrust and Brexit narratives at a local level
Authors: Olivas Osuna José Javier , Kiefel Max , Gartzou Katsouyanni Kira 
Published: July 2021

AbstractBased on comparative qualitative research in five local authority areas, this article argues that local context is key to understanding the roots of the U.K.'s crisis of political trust and the result of the 2016 E.U. referendum. The competing cultural and economic causes of discontent suggested by the literature were found to be deeply intertwined when analyzed from a local perspective. The sense of political disempowerment and negative attitudes toward migration were ingrained in and reinforced by locally specific socio‐economic and political trajectories. These experiences were articulated and amplified by dominant discourses, which channeled frustration against the political elite and the E.U. These populist narratives, promoted by the Leave campaign and the tabloid press, became dominant in certain areas, decisively shaping citizens' voting behavior. Overall, the article highlights the value of studying how local experiences and interpretations mediate the interplay of cultural and economic causes of discontent and political distrust.
Gender apartheid: The challenges of breaking into “man's world”
Authors: Adisa Toyin Ajibade , Gbadamosi Gbolahan , Adekoya Olatunji David 
Published: July 2021

AbstractDrawing on feminist/gender theory and the notion that women self‐allocate to occupations with which they identify, this study examines the challenges confronted by women working in male‐dominated occupations in a highly patriarchal setting. The findings reveal that women working in “male‐typed” occupations in Nigeria are “lone wolves” in a marginal revolution. They experience social ostracism, impaired spousal relationships, and a lack of support from friends and family. Furthermore, the entrenchment of patriarchal culture, the requirement of physical strength, and the lack of trust on the part of clients pose significant barriers to their success in male‐dominated professions. These problems further breed and reinforce occupational gender segregation. The prospects of reducing occupational gender segregation and the underrepresentation of women in “male‐typed” occupations appear slim due to cultural beliefs and the patriarchal orientation in the study context.
PRAME expression in melanocytic lesions of the conjunctiva
Authors: Šekoranja Daja , Hawlina Gregor , Pižem Jože 
Published: July 2021

AimsPRAME (PReferentially expressed Antigen in MElanoma) is a tumour‐associated antigen that is preferentially strongly expressed in most cutaneous melanomas but not or only focally in naevi. Our aim was to evaluate PRAME expression in melanocytic lesions of the conjunctiva.Methods and resultsSurgical specimens of 114 conjunctival melanocytic naevi of different types (including 67 common, 25 combined deep penetrating and 21 inflamed juvenile naevi), 30 invasive melanomas, 10 in‐situ melanomas, 23 primary acquired melanoses (PAM) without atypia and 11 PAM with atypia were analysed for PRAME expression by immunohistochemistry. Nuclear positivity for PRAME in melanocytes was assessed as the percentage of positive nuclei: negative (0%), 1+ (1–25%), 2+ (26–50%), 3+ (51–75%) and 4+ (> 75%). In 113 of 114 conjunctival melanocytic naevi, PRAME was either completely negative or focally 1+ positive. Diffuse 4+ PRAME expression was identified in 17 of 30 (57%) invasive melanomas, seven of 10 (70%) in‐situ melanomas, four of five (80%) PAM with severe atypia, none of three PAM with moderate atypia, none of three PAM with mild atypia, one of 23 (4%) PAM without atypia and none of 114 naevi. Diffuse 4+ PRAME expression in invasive melanomas correlated with a higher mitotic count but was not related to age and gender of the patients, Breslow thickness, location or mutational status.ConclusionDiffuse 4+ PRAME positivity is highly specific for malignant conjunctival melanocytic lesions. PRAME is therefore a useful ancillary marker to support the diagnosis of a suspected conjunctival melanoma.