bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case report of unexpected right-to-left shunt under mechanical support for post-infarction ventricular septal defect: evaluation with haemodynamic simulator
Authors: Hiraoka Arudo , Saku Keita , Nishikawa Takuya , Sunagawa Kenji 
Published: August 2021

Abstract

Background
Post-myocardial infarction ventricular septal defect (PIVSD) is a complication of acute myocardial infarction with high mortality. A percutaneous left ventricular assist device, Impella, is currently used in maintaining haemodynamic stability in PIVSD.

Case summary
A 65-year-old man was transferred to our hospital for treatment of acute myocardial infarction of the proximal right coronary artery. Percutaneous intervention was performed but haemodynamic instability continued. At 10 days after onset, the patient was diagnosed with PIVSD by echocardiogram. To stabilize haemodynamics, we initiated venoarterial extracorporeal membrane oxygenation (ECMO). Three days after ECMO initiation, pulmonary congestion increased and an echocardiogram revealed closed aortic valve and spontaneous echo contrast at the aortic root. After an Impella 2.5 was inserted for unloading of the left ventricle, the oxygenation level and cardiac function rapidly declined. Unexpectedly, an echocardiogram showed a right-to-left shunt (to-and-fro pattern) via PIVSD. By increasing the ECMO and decreasing Impella flow, the shunt flow changed to left-to-right, and oxygenation level and cardiac function improved. Ten days after ECMO was started, elective surgical repair was successfully performed.

Conclusion
ECPELLA (ECMO + Impella) can offset the adverse effects of isolated ECMO support and reduce the PIVSD shunt flow. However, the risk of right-to-left shunt has not been reported, and ECPELLA caused a right-to-left shunt with deoxygenated systemic perfusion in the present case. A simulation study indicated that the right ventricular failure in PIVSD may pose a risk for right-to-left PIVSD shunt under Impella support.
Anticholinergic Medication Use, Dopaminergic Genotype, and Recurrent Falls
Authors: Rosso Andrea L , Marcum Zachary A , Zhu Xiaonan , Bohnen Nicolaas , Rosano Caterina 
Published: August 2021

Abstract

Background
Anticholinergic medications are associated with fall risk. Higher dopaminergic signaling may provide resilience to these effects. We tested interactions between anticholinergic medication use and dopaminergic genotype on risk for recurrent falls over 10 years.

Methods
Participants in the Health, Aging, and Body Composition (Health ABC) study (n = 2 372, mean age = 73.6; 47.8% men; 60.0% White) without disability or anticholinergic use at baseline were followed for up to 10 years for falls. Medication use was documented in 7 of 10 years. Highly anticholinergic medications were defined by Beers criteria, 2019. Recurrent falls were defined as ≥2 in the 12 months following medication assessment. Generalized estimating equations tested the association of anticholinergic use with recurrent falls in the following 12 months, adjusted for demographics, health characteristics, and anticholinergic use indicators. Effect modification by dopaminergic genotype (catechol-O-methyltransferase [COMT]; Met/Met, higher dopamine signaling, n = 454 vs Val carriers, lower dopamine signaling, n = 1 918) was tested and analyses repeated stratified by genotype.

Results
During follow-up, 841 people reported recurrent falls. Anticholinergic use doubled the odds of recurrent falls (adjusted odds ratio [OR] [95% CI] = 2.09 [1.45, 3.03]), with suggested effect modification by COMT (p = .1). The association was present in Val carriers (adjusted OR [95% CI] = 2.16 [1.44, 3.23]), but not in Met/Met genotype (adjusted OR [95% CI] = 1.70 [0.66, 4.41]). Effect sizes were stronger when excluding baseline recurrent fallers.

Conclusion
Higher dopaminergic signaling may provide protection against increased 12-month fall risk from anticholinergic use. Assessing vulnerability to the adverse effects of anticholinergic medications could help in determination of risk/benefit ratio for prescribing and deprescribing anticholinergics in older adults.
MultiInflammatory Syndrome in Children: A View into Immune Pathogenesis from a Laboratory Perspective
Authors: Bohn Mary Kathryn , Yousef Peter , Steele Shannon , Sepiashvili Lusia , Adeli Khosrow 
Published: August 2021

Abstract

Background
Multiinflammatory syndrome in children (MIS-C) is a novel and rare inflammatory disorder associated with severe acute respiratory syndrome coronavirus 2 infection in school-age children. Reports in the past year have suggested a multisystem pathophysiology characterized by hyperinflammation, gastrointestinal distress, and cardiovascular complications. Clinical laboratory investigations, including routine blood testing for inflammatory (e.g., C-reactive protein, ferritin) and cardiac (e.g., troponin, brain natriuretic peptides) markers have provided insight into potential drivers of disease pathogenesis, highlighting the role of the laboratory in the differential diagnosis of patients presenting with similar conditions (e.g., Kawasaki disease, macrophage activating syndrome).

Content
While few studies have applied high-dimensional immune profiling to further characterize underlying MIS-C pathophysiology, much remains unknown regarding predisposing risk factors, etiology, and long-term impact of disease onset. The extent of autoimmune involvement is also unclear. In the current review, we summarize and critically evaluate available literature on potential pathogenic mechanisms underlying MIS-C onset and discuss the current and anticipated value of various laboratory testing paradigms in MIS-C diagnosis and monitoring.

Summary
From initial reports, it is clear that MIS-C has unique inflammatory signatures involving both adaptive and innate systems. Certain cytokines, inflammatory markers, and cardiac markers assist in the differentiation of MIS-C from other hyperinflammatory conditions. However, there are still major gaps in our understanding of MIS-C pathogenesis, including T cell, B cell, and innate response. It is essential that researchers not only continue to decipher initial pathogenesis but also monitor long-term health outcomes, particularly given observed presence of circulating autoantibodies with unknown impact.
Newspaper Coverage of Snus in an Emerging Norwegian Snus Market 2002–2011: A Content Analysis
Authors: Gunnar Sæbø , Rikke Iren Tokle , Ingeborg Lund 
Published: August 2021

Abstract

Background
In a context where snus is a legal product, its advertising is prohibited and its prevalence of use has been on the rise among adolescents and young adults, the aim of this article is to identify the extent of snus coverage in Norwegian newspapers and the themes and values communicated about snus therein from 2002 to 2011.

Aim and methods
All major Norwegian newspapers were scanned for articles with “snus” (and relevant connectors) in headings, ingresses, and/or pictures/captions as search criteria. Using the Retriever media monitoring service as a database, the search returned 943 unique articles, which were subjected to quantitative content analysis.

Results
The number of articles per year increases over the period, while their average length decreases slightly. Thematically, the greatest attention is on the extent of “snus use” (occurring in 52.7% of the articles), and then more equally divided between “tobacco policy” (24.5%), “economy/markets” (29.1%), and “health” (28.7%). A total of 48.6% of the articles are “neutral/mixed” in respect of framing, 28.1% are “negative,” and only 20.7% are “positive” in tone. Articles about tobacco policy are more often negative, while articles on economic factors are more often positive. Articles on health are usually negatively focused, or neutral/mixed.

Conclusion
The slight predominance of negative and/or neutral/mixed articles indicates that the newspaper coverage does not glamorize the snus product. However, the sheer amount of (and growth in) articles over time, as well as positive articles available for selective exposure and perception, may nevertheless have contributed to a normalization of snus use.

Implications
Little is known about media coverage of smokeless tobacco and whether editorial mass media glamorize or criticize its use. This study shows that the extent of snus coverage in Norwegian newspapers has increased over time, but also that the framing of Norwegian newspaper coverage of snus has mainly been neutral/mixed or negative toward snus and its use.
CaAIL1 Acts Positively in Pepper Immunity against Ralstonia solanacearum by Repressing Negative Regulators
Authors: Zheng Yutong , He Shicong , Cai Weiwei , Shen Lei , Huang Xueying , Yang Sheng , Huang Yu , Lu Qiaoling , Wang Hui , Guan Deyi , He Shuilin 
Published: August 2021

Abstract
APETALA2 (AP2) subfamily transcription factors participate in plant growth and development, but their roles in plant immunity remain unclear. Here, we discovered that the AP2 transcription factor CaAIL1 functions in immunity against Ralstonia solanacearum infection (RSI) in pepper (Capsicum annuum). CaAIL1 expression was upregulated by RSI, and loss- and gain-of-function assays using virus-induced gene silencing and transient overexpression, respectively, revealed that CaAIL1 plays a positive role in immunity to RSI in pepper. Chromatin immunoprecipitation sequencing (ChIP-seq) uncovered a subset of transcription-factor-encoding genes, including CaRAP2-7, CaGATA17, CaGtf3a and CaTCF25, that were directly targeted by CaAIL1 via their cis-elements, such as GT or AGGCA motifs. ChIP-qPCR and electrophoretic mobility shift assays confirmed these findings. These genes, encoding transcription factors with negative roles in immunity, were repressed by CaAIL1 during pepper response to RSI, whereas genes encoding positive immune regulators such as CaEAS were derepressed by CaAIL1. Importantly, we showed that the atypical EAR motif (LXXLXXLXX) in CaAIL1 is indispensable for its function in immunity. These findings indicate that CaAIL1 enhances the immunity of pepper against RSI by repressing a subset of negative immune regulators during the RSI response through its binding to several cis-elements in their promoters.
Centers for Mendelian Genomics: A decade of facilitating gene discovery
Authors: Baxter Samantha M. , Posey Jennifer E. , Lake Nicole J. , Sobreira Nara , Chong Jessica X. , Buyske Steven , Blue Elizabeth E. , Chadwick Lisa H. , Coban-Akdemir Zeynep H. , Doheny Kimberly F. , Davis Colleen P. , Lek Monkol , Wellington Christopher , Jhangiani Shalini N. , Gerstein Mark , Gibbs Richard A. , Lifton Richard P. , MacArthur Daniel G. , Matise Tara C. , Lupski James R. , Valle David , Bamshad Michael J. , Hamosh Ada , Mane Shrikant , Nickerson Deborah A. ,   Centers for Mendelian Genomics Consortium, Rehm Heidi L. , O’Donnell-Luria Anne 
Published: August 2021

AbstractMendelian disease genomic research has undergone a massive transformation over the last decade. With increasing availability of exome and genome sequencing, the role of Mendelian research has expanded beyond data collection, sequencing, and analysis to worldwide data sharing and collaboration. Over the last 10 years, the NIH-supported Centers for Mendelian Genomics (CMGs) have played a major role in this research and clinical evolution. We highlight the cumulative gene discoveries facilitated by the program, biomedical research leveraged by the approach, and the larger impact on the research community. Mendelian genomic research extends beyond generating lists of gene-phenotype relationships, it includes developing tools, training the larger community to use these tools and approaches, and facilitating collaboration through data sharing. Thus, the CMGs have also focused on creating resources, tools, and training for the larger community to foster the understanding of genes and genome variation. The CMGs have participated in a wide range of data sharing activities, including deposition of all eligible CMG data into AnVIL (NHGRI’s Genomic Data Science Analysis, Visualization, and Informatics Lab-Space), sharing candidate genes through Matchmaker Exchange (MME) and the CMG website, and sharing variants in Geno2MP and VariantMatcher. The research genomics output remains exploratory with evidence that thousands of disease genes, in which variant alleles contribute to disease, remain undiscovered, and many patients with rare disease remain molecularly undiagnosed. Strengthening communication between research and clinical labs, continued development and sharing of knowledge and tools required for solving previously unsolved cases, and improving access to data sets, including high-quality metadata, are all required to continue to advance Mendelian genomics research and continue to leverage the Human Genome Project for basic biomedical science research and clinical utility.
Short Term Reduction in the Odds of Testing Positive for SARS-CoV-2; a Comparison Between Two Doses and Three doses of the BNT162b2 Vaccine
Authors: Patalon Tal , Gazit Sivan , Pitzer Virginia E. , Prunas Ottavia , Warren Joshua L. , Weinberger Daniel M. 
Published: August 2021

AbstractWith the evidence of waning immunity of the BNT162b2 vaccine, a national third dose vaccination campaign was initiated in Israel during August 2021; other countries have announced their intention to administer a booster shot as well. Leveraging data from Maccabi Healthcare Services, we conducted a preliminary retrospective study aimed at evaluating initial short-term effectiveness of a three dose versus a two dose regimen against infection due to the Delta variant of SARS-CoV-2, using two complementary approaches; a test-negative design and a matched case-control design. We found that 7-13 days after the booster shot there is a 48-68% reduction in the odds of testing positive for SARS-CoV-2 infection and that 14-20 days after the booster the marginal effectiveness increases to 70-84%. Further studies are needed to determine the duration of protection conferred by the third dose and its effect on severe disease.
Revealing the genetic components responsible for the unique photosynthetic stem capability of the wild almond Prunus arabica (Olivier) Meikle
Authors: Brukental Hillel , Doron-Faigenboim Adi , Bar-Ya’akov Irit , Harel-Beja Rotem , Attia Ziv , Azoulay-Shemer Tamar , Holland Doron 
Published: August 2021

AbstractAlmond (Prunus dulcis (Mill.) D. A. Webb) is a major deciduous fruit tree crop worldwide. During dormancy, under warmer temperatures and inadequate chilling hours, the plant metabolic activity increases and may lead to carbohydrate deficiency. Prunus arabica (Olivier) Meikle is a bushy wild almond species known for its green, un-barked stem, which stays green even during the dormancy period. Our study revealed that P. arabica green stems assimilate significantly high rates of CO2 during the winter as compared to P. dulcis cv. Um el Fahem (U.E.F), and may improve carbohydrate status throughout dormancy. To uncover the genetic inheritance and mechanism behind the P. arabicaStem Photosynthetic Capability (SPC), a segregated F1 population was generated by crossing P. arabica to U.E.F. Both parent’s whole genome was sequenced, and a single nucleotide polymorphism (SNP) calling identified 4,887 informative SNPs for genotyping. A robust genetic map for U.E.F and P. arabica was constructed (971 and 571 markers, respectively). QTL mapping and association study for the SPC phenotype revealed major QTL (log of odd (LOD)=20.8) on chromosome 7, and another minor but significant QTL on chromosome 1 (LOD=3.9). Finally, a list of 73 candidate genes was generated. This work sets the stage for future research to investigate the mechanism regulating the SPC trait, how it affects the tree’s physiology, and its importance for breeding new cultivars better adapted to high winter temperatures.
Idea Paper: Effects of gonad type and body mass on the time required for sex change in fishes
Authors: Tokunaga Soma , Kadota Tatsuru , Watanabe Yuuki Y. , Kuwamura Tetsuo , Kawabata Yuuki 
Published: August 2021

AbstractSex change is a well-known phenomenon in teleost fishes, and it takes several days to a few months depending on the species and direction of sex change. However, the underlying factors influencing the time required for sex change (TS) remain unclear. Given that the time for producing a new gonad largely determines TS, the gonad type (i.e., whether fish retain the gonad of opposite sex or not [delimited or non-delimited]) and metabolic rate are the ultimate determinants of TS. This study sought to test two hypotheses: (1) the delimited gonad shortens TS; and (2) TS scales with mass0.1–0.2, because the metabolic scaling exponent (β) in fishes is 0.8–0.9 and biological times scale with mass1−β in general. We compiled data on TS for 12 female-to-male and 14 male-to-female sex-changing species from the literature. Results of individual examinations of the effects of gonad type and mass were consistent with our hypotheses. However, upon simultaneous examination of the effects of gonad type and mass, these effects became unclear because of their strong multicollinearity. The compiled data for delimited and non-delimited gonads were biased toward the smaller and larger species, respectively, precluding us from being able to statistically distinguish between these effects. Small species with non-delimited gonads and large species with delimited gonads exist; however, their TS has not been measured with high temporal resolution thus far. Therefore, additional experiments on these species are required to statistically distinguish between, as well as to better understand, the effects of gonad type and mass on TS.
An accelerated thrombosis model for computational fluid dynamics simulations in rotary blood pumps
Authors: Blum Christopher , Groß-Hardt Sascha , Steinseifer Ulrich , Neidlin Michael 
Published: August 2021

AbstractPurposeThrombosis is one of the major complications in blood-carrying medical devices and a better understanding to influence design of such devices is desirable. Over the past years many computational models of thrombosis have been developed. However, open questions remain about the applicability and implementation within a pump development process. The aim of the study was to develop and test a computationally efficient model for thrombus risk prediction in rotary blood pumps.MethodsWe used a two-stage approach to calculate thrombus risk. At the first stage, the velocity and pressure fields were computed by computational fluid dynamic (CFD) simulations. At the second stage, platelet activation by mechanical and chemical stimuli was determined through species transport with an Eulerian approach. The model was implemented in ANSYS CFX and compared with existing clinical data on thrombus deposition within the HeartMate II.ResultsOur model shows good correlation (R2>0.94) with clinical data and identifies the bearing and outlet stator region of the HeartMate II as the location most prone to thrombus formation. The calculation of platelet activation requires an additional 10-20 core hours of computation time.DiscussionThe concentration of activated platelets can be used as a surrogate marker to determine risk regions of thrombus deposition in a blood pump. Model expansion, e.g. by including more chemical species can easily be performed. We make our model openly available by implementing it for the FDA benchmark blood pump.DeclarationsFundingThis research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. Open access funding enabled and organized by Projekt DEAL.Conflict of interestAll of the authors have nothing to disclose.Availability of data and materialThe raw data can be retrieved by request from the authors.Code availabilityThe implementation of the thrombus model in the FDA benchmark blood pump geometry is available onhttps://doi.org/10.5281/zenodo.5116063.Authors’ contributionsAll authors contributed to the study conception and design. CB developed the numerical model, performed the simulations, gathered, analysed and discussed the results. SGH, MN and US were involved in the analysis and discussion of the results. MN supervised the project. MN and CB wrote the manuscript based on the input of all co-authors. All co-authors read and approved the final version of the manuscript.
Cosmopolitanism at the Roman Danubian Frontier, Slavic Migrations, and the Genomic Formation of Modern Balkan Peoples
Authors: Olalde Iñigo , Carrión Pablo , Mikić Ilija , Rohland Nadin , Mallick Shop , Lazaridis Iosif , Korać Miomir , Golubović Snežana , Petković Sofija , Miladinović-Radmilović Nataša , Vulović Dragana , Stewardson Kristin , Lawson Ann Marie , Zalzala Fatma , Callan Kim , Tomanović Željko , Keckarević Dušan , Grbić Miodrag , Lalueza-Fox Carles , Reich David 
Published: August 2021

AbstractThe Roman Empire expanded through the Mediterranean shores and brought human mobility and cosmopolitanism across this inland sea to an unprecedented scale. However, if this was also common at the Empire frontiers remains undetermined. The Balkans and Danube River were of strategic importance for the Romans acting as an East-West connection and as a defense line against “barbarian” tribes. We generated genome-wide data from 70 ancient individuals from present-day Serbia dated to the first millennium CE; including Viminacium, capital of Moesia Superior province. Our analyses reveal large scale-movements from Anatolia during Imperial rule, similar to the pattern observed in Rome, and cases of individual mobility from as far as East Africa. Between ∼250-500 CE, we detect gene-flow from Central/Northern Europe harboring admixtures of Iron Age steppe groups. Tenth-century CE individuals harbored North-Eastern European-related ancestry likely associated to Slavic-speakers, which contributed >20% of the ancestry of today’s Balkan people.
Intravenous administration of BCG protects mice against lethal SARS-CoV-2 challenge
Authors: Hilligan Kerry L. , Namasivayam Sivaranjani , Clancy Chad S. , O’Mard Danielle , Oland Sandra D. , Robertson Shelly J. , Baker Paul J. , Castro Ehydel , Garza Nicole L. , Lafont Bernard A. P. , Johnson Reed , Ronchese Franca , Mayer-Barber Katrin D. , Best Sonja M. , Sher Alan 
Published: August 2021

AbstractEarly events in the host response to SARS-CoV-2 are thought to play a major role in determining disease severity. During pulmonary infection, the virus encounters both myeloid and epithelioid lineage cells that can either support or restrict pathogen replication as well as respond with host protective versus detrimental mediators. In addition to providing partial protection against pediatric tuberculosis, vaccination with bacille Calmette-Guérin (BCG) has been reported to confer non-specific resistance to unrelated pulmonary pathogens, a phenomenon attributed to the induction of long-lasting alterations within the myeloid cell compartment. Here we demonstrate that prior intravenous, but not subcutaneous, administration of BCG protects human-ACE2 transgenic mice against lethal challenge with SARS-CoV-2 and results in reduced viral loads in non-transgenic animals infected with an alpha variant. The observed increase in host resistance was associated with reductions in SARS-CoV-2-induced tissue pathology, inflammatory cell recruitment and cytokine production that multivariate analysis revealed to be only partially related to diminished viral load. We propose that this protection stems from BCG-induced alterations in the composition and function of the pulmonary cellular compartment that impact the innate response to the virus and the ensuing immunopathology.