bluuebunny/crossref_metadata_2025_split huggingface dataset:
A MicroRNA-Based Network Provides Potential Predictive Signatures and Reveals the Crucial Role of PI3K/AKT Signaling for Hepatic Lineage Maturation
Authors: Wang Xicheng , Zhang Wencheng , Yang Yong , Wang Jiansong , Qiu Hua , Liao Lijun , Oikawa Tsunekazu , Wauthier Eliane , Sethupathy Praveen , Reid Lola M. , Liu Zhongmin , He Zhiying 
Published: June 2021

BackgroundFunctions of miRNAs involved in tumorigenesis are well reported, yet, their roles in normal cell lineage commitment remain ambiguous. Here, we investigated a specific “transcription factor (TF)-miRNA-Target” regulatory network during the lineage maturation of biliary tree stem cells (BTSCs) into adult hepatocytes (hAHeps).MethodBioinformatic analysis was conducted based on our RNA-seq and microRNA-seq datasets with four human hepatic-lineage cell lines, including hBTSCs, hepatic stem cells (hHpSCs), hepatoblasts (hHBs), and hAHeps. Short time-series expression miner (STEM) analysis was performed to reveal the time-dependent dynamically changed miRNAs and mRNAs. GO and KEGG analyses were applied to reveal the potential function of key miRNAs and mRNAs. Then, the miRDB, miRTarBase, TargetScan, miRWalk, and DIANA-microT-CDS databases were adopted to predict the potential targets of miRNAs while the TransmiR v2.0 database was used to obtain the experimentally supported TFs that regulate miRNAs. The TCGA, Kaplan–Meier Plotter, and human protein atlas (HPA) databases and more than 10 sequencing data, including bulk RNA-seq, microRNA-seq, and scRNA-seq data related to hepatic development or lineage reprogramming, were obtained from both our or other published studies for validation.ResultsSTEM analysis showed that during the maturation from hBTSCs to hAHeps, 52 miRNAs were downwardly expressed and 928 mRNA were upwardly expressed. Enrichment analyses revealed that those 52 miRNAs acted as pluripotency regulators for stem cells and participated in various novel signaling pathways, including PI3K/AKT, MAPK, and etc., while 928 mRNAs played important roles in liver-functional metabolism. With an extensive sorting of those key miRNAs and mRNAs based on the target prediction results, 23 genes were obtained which not only functioned as the targets of 17 miRNAs but were considered critical for the hepatic lineage commitment. A “TF-miRNA-Target” regulatory network for hepatic lineage commitment was therefore established and had been well validated by various datasets. The network revealed that the PI3K/AKT pathway was gradually suppressed during the hepatic commitment.ConclusionA total of 17 miRNAs act as suppressors during hepatic maturation mainly by regulating 23 targets and modulating the PI3K/AKT signaling pathway. The regulatory network uncovers possible signatures and guidelines enabling us to identify or obtain the functional hepatocytes for future study.
Chronic Jet Lag Exacerbates Jejunal and Colonic Microenvironment in Mice
Authors: Li Qing , Wang Bo , Qiu Hong-Yi , Yan Xiu-Juan , Cheng Li , Wang Qian-Qian , Chen Sheng-Liang 
Published: June 2021

BackgroundEvidence suggests that circadian rhythm disorder is associated with a variety of gastrointestinal diseases, and the circadian rhythm plays a key role in maintaining the homeostasis of intestinal flora. The underlying mechanisms are still not completely identified. This study was aimed to explore whether jet lag-caused circadian disruption influences gut microbiome and its metabolites.MethodsMice were synchronized with 12-h light/dark cycles (control group) or subjected to daily 8-h advance of the light/dark cycle for every 3 days (jet-lagged group). Four months later, fecal samples and jejunal contents were collected and analyzed by 16S rRNA gene sequencing. In addition, fecal samples were subjected to metabolome analysis with ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS).ResultsThe results of 16s rRNA sequencing showed that chronic jet lag led to decreased microbial abundance, richness, and diversity in both feces and jejunal contents. ANOSIM analysis revealed significant difference between control and jet-lagged groups. As the colonic microbiome, the abundance of Bacteroidetes phylum was significantly decreased and that of Actinobacteria phylum was increased in jet-lagged mice. Jet lag increased the ratio of Firmicutes to Bacteroidetes, an indicator for the imbalance of gut microbiota. Metabolome analysis of fecal samples showed that the levels of tryptophan and its derivatives were decreased in jet-lagged mice. In addition, fecal levels of secondary bile acids changed under jet lag conditions. Correlation analysis identified associations between tryptophan (and its derivatives) levels and colonic microbiota.ConclusionsThis study presents a comprehensive landscape of gut microbiota and its metabolites in mice subjected to chronic jet lag. The results suggest that circadian disruption may lead to changes in fecal and jejunal microbiota and fecal metabolites. Moreover, our results demonstrate a novel interplay between the gut microbiome and metabolome.
Viability and Infectivity of Plasmodium vivax Gametocytes in Short-Term Culture
Authors: Ramos Glenda Quaresma , Baia-da-Silva Djane Clarys , Lacerda Marcus Vinícius Guimarães , Monteiro Wuelton Marcelo , Lopes Stefanie Costa Pinto 
Published: June 2021

The control and elimination of malaria caused by Plasmodium vivax both represent a great challenge due to the biological aspects of the species. Gametocytes are the forms responsible for the transmission of the parasite to the vector and the search for new strategies for blocking transmission are essential in a scenario of control and elimination The challenges in this search in regard to P. vivax mainly stem from the lack of a long-term culture and the limitation of studies of gametocytes. This study evaluated the viability and infectivity of P. vivax gametocytes in short-term culture. The samples enriched in gametocytes using Percoll (i), using magnetic-activated cell sorting (MACS®) (ii), and using non-enriched samples (iii) were evaluated. After the procedures, gametocytes were cultured in IMDM medium for up to 48 h. Cultured P. vivax gametocytes were viable and infectious for up to 48 h, however differences in viability and infectivity were observed in the samples after 12 h of culture in relation to 0 h. Percoll-enriched samples were shown to be viable in culture for longer intervals than those purified using MACS®. Gametocyte viability after enrichment procedures and short-term culture may provide new avenues in the development of methods for evaluating P. vivax TB.
Patient Safety Through Nursing Documentation: Barriers Identified by Healthcare Professionals and Students
Authors: Bjerkan Jorunn , Valderaune Victor , Olsen Rose Mari 
Published: June 2021

Background: Although access to accurate patient documentation is recognized as a prerequisite for delivering of safe and continuous municipal elderly care, healthcare professionals often fail to provide comprehensive clinical information in an accurate and timely manner. The aim of this study was to understand the perceptions of healthcare professionals and healthcare students regarding existing barriers to patient safety through the performance of documentation practices.Methods: Using a qualitative, exploratory design, this study conducted six focus group interviews with nurses and social educators (n = 12) involved in primary care practice and nursing and social educator bachelor’s degree students from a University College (n = 11). Data were analyzed using qualitative content analysis.Results: Four themes emerged from the analysis, which described barriers to patient safety and quality in documentation practices: “Individual factors,” “Social factors,” “Organizational factors,” and “Technological factors.” Each theme also included several sub-themes.Conclusion: According to the findings, several barriers negatively influenced documentation practices and information exchange, which may place primary care patients in a vulnerable and exposed situation. To achieve successful documentation, increased awareness and efforts by the individual professional are necessary. However, primary care services must facilitate the achievement of these goals by providing adequate resources, clear mission statements, and understandable policies.
A High-Protein, Low Glycemic Index Diet Suppresses Hunger but Not Weight Regain After Weight Loss: Results From a Large, 3-Years Randomized Trial (PREVIEW)
Authors: Zhu Ruixin , Fogelholm Mikael , Larsen Thomas M. , Poppitt Sally D. , Silvestre Marta P. , Vestentoft Pia S. , Jalo Elli , Navas-Carretero Santiago , Huttunen-Lenz Maija , Taylor Moira A. , Stratton Gareth , Swindell Nils , Kaartinen Niina E. , Lam Tony , Handjieva-Darlenska Teodora , Handjiev Svetoslav , Schlicht Wolfgang , Martinez J. Alfredo , Seimon Radhika V. , Sainsbury Amanda , Macdonald Ian A. , Westerterp-Plantenga Margriet S. , Brand-Miller Jennie , Raben Anne 
Published: June 2021

Background: Previous studies have shown an increase in hunger during weight-loss maintenance (WLM) after diet-induced weight loss. Whether a combination of a higher protein, lower glycemic index (GI) diet and physical activity (PA) can counteract this change remains unclear.Aim: To compare the long-term effects of two diets [high protein (HP)-low GI vs. moderate protein (MP)-moderate GI] and two PA programs [high intensity (HI) vs. moderate intensity (MI)] on subjective appetite sensations during WLM after ≥8% weight loss (WL).Methods: Data derived from the 3-years PREVIEW randomized intervention study. An 8-weeks WL phase using a low-energy diet was followed by a 148-weeks randomized WLM phase. For the WLM phase, participants were assigned to one of the four groups: HP-MI, HP-HI, MP-MI, and MP-HI. Available data from 2,223 participants with overweight or obesity (68% women; BMI ≥ 25 kg/m2). Appetite sensations including satiety, hunger, desire to eat, and desire to eat something sweet during the two phases (at 0, 8 weeks and 26, 52, 104, and 156 weeks) were assessed based on the recall of feelings during the previous week using visual analogue scales. Differences in changes in appetite sensations from baseline between the groups were determined using linear mixed models with repeated measures.Results: There was no significant diet × PA interaction. From 52 weeks onwards, decreases in hunger were significantly greater in HP-low GI than MP-moderate GI (Ptime × diet = 0.018, Pdietgroup = 0.021). Although there was no difference in weight regain between the diet groups (Ptime × diet = 0.630), hunger and satiety ratings correlated with changes in body weight at most timepoints. There were no significant differences in appetite sensations between the two PA groups. Decreases in hunger ratings were greater at 52 and 104 weeks in HP-HI vs. MP-HI, and greater at 104 and 156 weeks in HP-HI vs. MP-MI.Conclusions: This is the first long-term, large-scale randomized intervention to report that a HP-low GI diet was superior in preventing an increase in hunger, but not weight regain, during 3-years WLM compared with a MP-moderate GI diet. Similarly, HP-HI outperformed MP-HI in suppressing hunger. The role of exercise intensity requires further investigation.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT01777893.
The Efficacy and Safety of Esmolol for Septic Shock: A Systematic Review and Meta-analysis of Randomized Controlled Trials
Authors: Huang Po , Zheng Xiangchun , Liu Zhi , Fang Xiaolei 
Published: June 2021

Purpose: The meta-analysis aims to identify whether septic shock patients can benefit from esmolol.Materials and Methods: The relevant studies from MEDLINE, Cochrane Library, Embase were searched by two independent investigators using a variety of keywords. Stata software (version 12.0, Stata Corp LP, College Station, TX, United States)was used for statistical analysis.Results: A total of 14 studies were identified and incorporated into the meta-analysis. For overall analysis, the treatment of esmolol was associated with decreased 28-day mortality (RR = 0.66, 95% CI = 0.56–0.77, p &lt; 0.001). Meanwhile, our analysis found that, esmolol could decrease HR (SMD: −1.70; 95% CI: [−2.24−(−1.17)], cTnI (SMD: −1.61; 95% CI: [−2.06−(−1.16)] compared with standard treatment. No significant differences between the two groups were found in MAP, Lac, CI, and SVI.Conclusion: The findings of this meta-analysis intend to demonstrate that septic shock patients with high heart beats rate might be benefit from esmolol treatment despite enough fluid resuscitation. While, dependent on the study published, with the further development of septic shock, the positive impact of esmolol varies. The appropriate heart rate change interval cannot be confirmed, further high-quality and large-scale RCTs should be performed to verify it and screening more suitable heart rate levels.Systematic Review Registration: CRD42021239513
Attitude of Medical Students About Their Role and Social Accountability in the COVID-19 Pandemic
Authors: Hong Jihoon , Jung Ikjae , Park Mingeol , Kim Kyumin , Yeo Sungook , Lee Joohee , Suh Sooyeon , Hong Youjin , Park Jangho , Chung Seockhoon 
Published: June 2021

Background:In this study, we aimed to explore the attitude of medical students toward their role and social accountability in this pandemic era. An online survey was developed to elicit information on (1) the role of medical students in the pandemic era; (2) Medical education in the “new normal,” and (3) the impact of COVID-19 on medical students.Methods:The online survey, developed by a team consisting of three medical students, three psychiatry residents, and three professors of psychiatry, was conducted on 574 participants (213 medical students, 180 graduates, and 181 professors) in the University of Ulsan College of Medicine, Seoul, South Korea. Anxiety symptom rating scales, including the Stress and Anxiety to Viral Epidemics-6 (SAVE-6) scale and the Generalized Anxiety Disorder−7 (GAD-7) scale, were applied to measure participant anxiety level.Results:Medical students indicated their willingness to join the healthcare response to the COVID-19 pandemic, if requested; however, graduates and professors recommended that medical students continue their training rather than join the pandemic healthcare response. In the new normal era, medical education has had to change appropriately. Moreover, adequate knowledge of COVID-19 infection and spread must be considered for the continuation of clinical clerkships during the pandemic. Overall, medical students who indicated anxiety about treating possible or confirmed cases of COVID-19 rated higher on the SAVE-6 scale. Finally, medical students who reported that COVID-19 had an impact on their studies and daily life rated higher on the general anxiety scale (GAD-7).Conclusion:Social accountability is an important issue for medical students in the pandemic era. At the same time, non-disruption of their academic calendar would ensure continuous availability of component medical professionals, which is important for adequate future healthcare responses.
Musculoskeletal Disorders Associated With Quality of Life and Body Composition in Urban and Rural Public School Teachers
Authors: Vega-Fernández Gustavo , Lera Lydia , Leyton Bárbara , Cortés Pilar , Lizana Pablo A. 
Published: June 2021

Introduction: Teachers have been reported to be a labor group with high rates of musculoskeletal disorders (MSDs), stress, and strong deterioration of quality of life (QoL). However, little information exists about the association between MSD, QoL, and body composition in rural and urban teachers.Objective: The aim was to study the association of MSD with QoL perception and body composition of urban and rural teachers.Participants and Methods: Participants are comprised a representative sample of urban and rural public schoolteachers from the Valparaiso Region, Chile. MSDs were evaluated with the Standardized Nordic Questionnaire for Musculoskeletal Symptoms validated for the Chilean population. QoL perception was evaluated with the 36-Item Short-Form Survey (SF-36). Body composition was measured via bioimpedance. A logistic regression model was used to evaluate the association between MSD, QoL, and body composition, adjusted for age and gender.Results: A total of 88.9% (urban 90%; rural 87%) of teachers felt pain in some body area, 71.2% of them with limitations; 39% of teachers presented body fat obesity, with the highest rate in rural women. The body area with the greatest MSD prevalence was the neck and shoulders (68.6%). Significant differences were observed between teachers with &gt;p75 of MSD (over six pain regions) and those with ≤p75 (six or fewer painful regions; p &lt; 0.05) on six QoL scales and on physical health components (PCSs) and mental health (MCS) in urban teachers. However, rural teachers presented no differences. The association between teachers with &gt;p75 MSD and low QoL perception was significant (p &lt; 0.05) in PCS and MCS. Furthermore, the regression model presents a significant association between rural areas and low PCS perception.Conclusions: Urban and rural teachers present high rates of MSD and obesity. Teachers with higher rates of MSD have their mental and physical QoL affected, making workplace intervention in MSD necessary to prevent teacher health deterioration.
Evaluation of egg quality parameters of two Hungarian ostrich populations
Authors: Brassó Lili Dóra , Komlósi István 
Published: June 2021

The aim of our study was to evaluate the quality parameters, porosity and weight loss of eggs deriving from the two most significant ostrich farms in Hungary. Quality parameters included weight, length, width, shape index, egg volume, surface area, circumference and shell volume. The effect of storage conditions in both farms and the incubation technology on egg weight loss in farm “A” were also examined. The research objective was to impart a comprehensive knowledge on egg quality parameters of the main ostrich populations in Hungary and to compare the trios and the farms with each other and the international literature. We could reveal significant differences between trios in all egg quality traits. In conclusion, the shorter and the narrower the eggs were, the more spherical shape they had. Narrower eggs showed smaller surface area, volume, circumference and shell volume and vice versa. Eggs from farm “B” indicated significantly greater width, shape index, surface area, circumference and shell volume than farm “A”. A significant difference was observed in weight loss during storage between the farms. Weight loss in farm “A” was a multiple of farm “B”. In farm “B” there was a weak, positive correlation between storage period and weight loss (r=0.22, P≤0.05), in farm “A” it was not significant (P=0.52). There was no relationship between the initial egg weight and weight loss either in farm “A” or farm “B” (P=0,21, P=0,69). A slight positive correlation could be noted between egg porosity and weight loss (r=0.24, P≤0.05). Pores count presented here was less than the international results. Poultry eggs contain the most pores at the blunt end, less via the equator and the least at the pointed end. In ostrich egg we found more pores via the equator against the blunt end. To draw more precise conclusions, further investigation should be carried out on porosity. Considering the fact that the length of storage period and the weight loss during incubation are in strict correlation with hatchability, we intend to extend our research aims to these traits.
ON INTERMEDIATE RINGS WHICH ARE FINITELY GENERATED MODULES OVER A NOETHERIAN RING
Authors: Linh Nguyễn Xuân , Thảo Lưu Phương 
Published: June 2021

Cho (R, m) là vành giao hoán Noether và Q(R) là vành các thương toàn phần của R. Mục đích của bài báo này là nghiên cứu cấu trúc của các vành trung gian giữa R và Q(R). Gọi X là tập tất cả các lớp tương đương [I], trong đó I là ideal của R sao cho I 2 = aI với a ∈ I là phần tử không là ước của không trong R. Gọi Y là tập tất cả các vành trung gian A giữa R và Q(R) sao cho A là R-môđun hữu hạn sinh. Trong bài báo này, chúng tôi thiết lập một song ánh từ X đến Y. Một số ví dụ được đưa ra để làm rõ kết quả. Thứ nhất, chúng tôi chỉ ra nếu R là một miền ideal chính thì R là phần tử duy nhất của Y. Thứ hai, cho một vành Buchsbaum R mà không là Cohen-Macaulay, chúng tôi xây dựng một vành trung gian Cohen-Macaulay A ∈ Y. Để giải quyết vấn đề, chúng tôi áp dụng phương pháp nghiên cứu của S. Goto năm 1983, L. T. Nhàn và M. Brodmann 2012.
PERUBAHAN WARNA IKAN MAS Cyprinus carpio DENGAN PENAMBAHAN EKSTRA BUAH PALA Myristica argentha PADA DOSIS BERBEDA
Authors: Budi Sutia , Mardiana Mardiana , Geris Geris , Tantu A. Gusti 
Published: June 2021

Ikan Hias jenis ikan Mas Koi Cyprinus carpio memiliki daya tarik pada warna yang dimunculkan dari tubuhnya. Dagung buah Pala Myristica argentha memiliki pigmen karotenoid, asam askorbat, flavenoid, phenol asit dan lutein. Penelitian ini bertujuan untuk mengetahui perubahan warna merah tubuh ikan Mas Koi Cyprinus carpio melalui pemberian ekstrak kasar pala dengan dosis yang berbeda. Parameter peubah yang diukur berupa perubahan warna dengan menggunakan scoring melalui metode modifikasi TCF. Wadah penelitian menggunakan toples transparan 5 L, dengan volume air sebanyak 3,5 L per wadah. Hewan uji yang digunakan adalah ikan Mas dengan panjang 10 ± 3,6 cm dengan kepadatan 1 ekor per wadah. Perlakuan yang di uji adalah berbagai konsentrasi ekstrak kasar buah pala dalam pakan, yakni A = 0 % , B = 5 %, C = 10 % dan D = 15 %, dengan masing – masing 3 kali ulangan. Hasil penelitian menunjukkan bahwa perlakuan berbagai konsentrasi ekstrak pala memberikan pengaruh terhadap perubahan warna merah tubuh ikan Mas Koi.
Análisis estadístico para validar parámetros de modelos matemáticos por medio método de mínimos cuadrados
Authors: Madroñero Madroñero Duver , Ibarguen Mondragón Eduardo , Vergel-Ortega Mawency 
Published: June 2021

En el presente artículo se aborda la estimación de parámetros en estadística frecuentista; por medio de regresiones lineales se estiman parámetros asociados a modelos matemáticos determinísticos, para dicho proceso se utiliza el método de mínimos cuadrados. Además, para la validación de los parámetros estimados mediante el método de mínimos cuadrados, se realiza un análisis estadístico en el cual se incluyen el coeficiente de determinación, desviación estándar, la prueba de t-student, entre otros. En otras palabras, este artículo pretende dar al lector una base de partida para aplicar un método sencillo y muy eficaz para estimar parámetros en modelos matemáticos.
El cuerpo como herramienta del intérprete en el performance musical
Authors: Noriega Peralta Saraí Stelia , Brenscheidt gen. Jost Diana 
Published: June 2021

Ubicándose teórica- y metodológicamente dentro de los estudios del per­formance, el interés de este trabajo se centra en la presencia corporal del intérprete en relación con los demás actores de un proceso musical, el cual transcurre siempre dentro de un distinto contexto social y cultural.
Partimos de un acercamiento a los estudios del performance, sus antecedentes en la lingüística, la ciencia del teatro y la antropología, para revisar en un segundo paso las repercusiones de esa corriente en la investigación musicológica actual.
Enfocándose en el mundo de la música occidental de concierto, específicamente el formato del recital, que trae consigo un catálogo de convenciones y reglas no-escritas referente al comportamiento social de todos sus actores, se investiga y analiza el traba­jo corporal de la cantante Barbara Hannigan en su interpretación de la obra Mysteries of the Macabre de György Ligeti, con la London Symphony Orchestra, bajo la batuta de Sir Simon Rattle.
Con este trabajo se busca observar una nueva propuesta del performance en el formato del recital, con el objetivo de destacar la importancia del entrenamiento y de la expre­sión corporal en el músico académico; así como cuestionar los cánones establecidos con la intensión de plantear la necesidad de nuevos elementos discursivos para el intér­prete, que vayan de acuerdo con las exigencias de la época actual.