bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Silico Study of The Potential of Naturally Caffeoylquinic Acids From Lentinus Crinitus Basidiocarp With Zika Virus Inhibition Targets
Authors: Fonseca Aluísio Marques da , Soares Neidelenio Baltazar , Colares Regilany Paulo , Coelho Ethanielda de Lima , Ribeiro Paulo Riceli Vasconcelos , de Souza João Sammy Nery , Alexandre Jeferson Yves Nunes Holanda , Sousa Cleiton , Marinho Emmanuel Silva 
Published: October 2021

Abstract
The methanolic extract of the fungus Lentinus crinitus was submitted to biological assays, identification of the chemical composition by LC-MS, and in silico study by molecular docking with all identified compounds. The test against Artemis salina reached LD50 > 1000 μg/mL within 24h, and total mortality within 48 hours; the antioxidant test 62.4% inhibition in 1.0 mg/mL was obtained. Only 16 compounds were identified from the LC-MS analysis based on the comparison of reports already recorded in the literature. Most of the compounds identified here are described for the first time in the genus Lentinus. These results showed that the fungus is a producer of different classes of secondary metabolites biologically active. The results of the molecular docking simulation of the identified phytochemicals presented 1,13,4-di-O-Caffeoylquinic, as the leading promising candidate in the inhibition of the Zika virus.
Impact On Vegetables Irrigated With Municipal and Industrial Wastewater From Korangi Drain Near IoBM, Karachi
Authors: Rabab Nida , Farooqi Abdulrauf , Soomro Shan e Hyder 
Published: October 2021

Abstract
The present study was conducted to assess the Impact on Vegetables Irrigated with Municipal and Industrial wastewater from Korangi Drain near IoBM, Karachi. Some vegetables are grown using sewage and industrial wastewater laden with alarmingly high levels of heavy metals and bacteriological contamination. Maximum concentration of lead was found in spinach 8.20 mg/l as against safe limits of 0.01 mg/l and maximum Nickel concentration was found in banana 3.114 mg/l as against 0.02mg/l whereas all vegetables were invariably bacteriologically contaminated much beyond safe limits. Appropriate legislations in Sindh and competent manpower for rigorous monitoring to gage the harmful impact on vegetables grown with untreated municipal and industrial wastewater to effectively combat the problems of growing vegetables. The emptying of untreated municipal and industrial wastewater through Korangi Drain in fresh water bodies into Karachi cost should be banned to save the coast becoming hypoxic causing irreparable loss to marine life.
Effect of Geometry on the Electromagnetic Wave Transport Properties of Photonic Crystals: Comparison of Top-Flat and Top-Curved Refractive Index Profiles
Authors: Solaimani M. , nejati malihe 
Published: October 2021

Abstract
In the current paper, we try to engineer the refractive index profile in a one-dimensional photonic crystal as a powerful tool to manage the electromagnetic wave transmission properties. For this purpose, we have compared four sinusoidal, rectangular, triangular, and saw-tooth refractive index profile types. In this way, we have used a transfer matrix method accompanied by the discretization of the spatial domain. This method can readily be applied to any arbitrary continuous refractive index profile. Then, we have tried to address the effects of different geometrical and physical parameters, including the photonic crystal length L, dielectric permittivity εd, number of layers and plasma density np, etc. on the light propagation through the mentioned photonic crystals. In the proposed two-layer plasma/dielectric photonic crystals we could observe acceptable ranges of Omni-directional photonic band gaps that their position width and their number can be regulated. We determine the most and least tunable systems.
Microstructure and Mechanical Performances Analyses on Probeless Friction Stir Spot Welds of AA6061-T6 Aluminum Sheets
Authors: Guishen Yu , Xin Chen , Zitao Wu , Guanchen Zhang , Yixiong Chen 
Published: October 2021

Abstract
Probeless friction stir spot welding (PFSSW) is a variation of conventional friction stir spot welding (CFSSW), which solves the keyhole defect in CFSSW welds. In this work, four types of probeless tools were designed based on helix and spiral feature: small curvature helix (involute tool, IT), large curvature helix (archimedes tool, AT), spiral line (scroll tool, ST) and no feature (flat tool, FT). AA6061-T6 sheets with a thickness of 1.5 mm were welded using the designed probeless welding tools. The effect of tool shapes on the microstructure and mechanical properties of friction stir spot welded AA 6061-T6 aluminum alloy sheets was investigated using different rotational speeds, plunging depths and dwell times. The Taguchi method was utilized to obtain the optimal welding parameter combination for the four tools. And, the tensile-shear mechanical properties of FSSW specimens were characterized under the optimal parameters. The results show that a basin-shaped profile appeared in the weld metallography using the groove tools. The tensile shear failure load (TSFL) of IT (~6.97 kN) was higher than that of ST (~6.49 kN), AT (~6.19 kN) and FT (~5.67 kN), sequentially. Three different fracture modes, interfacial fracture, plug fracture and mixed-mode fracture were observed in the tensile-shear tests of weld specimens.
The Relationship Between P16INK4A and TP53 Promoter Methylation and The Risk and Prognosis in Patients With Oesophageal Cancer in Thailand
Authors: Poosari Arisara , Nutravong Thitima , Namwat Wises , Wasenang Wiphawan , Sa-ngiamwibool Prakasit , Ungareewittaya Piti 
Published: October 2021

Abstract
DNA methylation can regulate the expression of tumour suppressor genes P16 and TP53, environmental factors, which are both important factors related to an increased risk and prognosis of oesophageal cancer (EC). However, the association between these two genes methylation status, as well as the effects of gene-environment interactions, EC risk remains unclear. A Hospital-based case-control study data were collected from 105 new EC cases and 108 controls. Promoter methylation status was investigated for P16 and TP53 genes using methylation-specific polymerase (MSP) chain reaction methods with SYBR green. Logistic and Cox regression models were used to analyse the association of P16 and TP53 promotor methylation status with EC risk and prognosis, respectively. Our results suggest P16, TP53 methylation significantly increased the risk of EC (OR = 5.24, 95 % CI: 2.57–10.66, P < 0.001; OR = 3.38, 95% CI: 1.17–6.67, P < 0.001, respectively). In addition, P16 and TP53 promoter methylation status and the combined effects between environmental factors and its methylations in tissue were correlated with the EC risk and prognosis of EC patients. As a new biomarker, the methylation of P16 and TP53 can serve as a potential predictive biomarker of EC.
Functional Role of RRS1 in the Chemosensitivity of Drug Resistant Breast Cancer Cell to Cisplatin
Authors: Peng Cuixiu , Wang Runze , Song Junying , Hua Yanan , Wu Qinglan , Deng Lin , Cao Yi , Zhang Li , Sun Fusheng , Hou Lin 
Published: October 2021

Abstract
RRS1(human regulator of ribosome synthesis 1), a critical nuclear protein participated in ribosome biogenesis, plays important roles in the genesis and development of breast cancer. Here, we reported that RRS1 was highly expressed in cisplatin resistant breast cancer cell MCF-7/DDP than that in parent MCF-7. RRS1 silencing increased the sensitivity of MCF-7/DDP cells to cisplatin, inhibited proliferation, affected cell cycle distribution and promoted apoptosis. Furthermore, in nude mice xenograft study, the content of RRS1 in cisplatin treatment group was significantly higher than that in saline treatment group. In addition, we found that RRS1 could bind to AEG-1 and subsequently strengthened AEG-1 abundance in breast cancer cells. Although AEG-1 did not affect AEG-1 gene transcription, it inhibited ubiquitination and subsequent proteasome-mediated degradation of AEG-1 protein. Our research in current study documented for the first time that RRS1 participated in the sensitivity of breast cancer cells to cisplatin through binding to AEG-1, indicating that RRS1 may be a promising target for the therapy of breast cancer.
Extremely Low Effective Impedance in Stratified Graphene-Dielectric Metamaterials
Authors: Hwang Ruey-Bing 
Published: October 2021

Abstract
The periodic reflections in frequency were observed in a stack of graphene layers and reported as a series of mini photonic bandgaps owing to the multiple interference by the graphene layers. In this research, the effective medium approach was employed to obtain the effective refractive index and Bloch impedance for understanding the wave propagation characteristic therein. Specifically, the pure real effective refractive index without attenuation as well as an extremely low Bloch impedance were found at the frequencies exhibiting periodic reflections. Some numerical examples were demonstrated to show that the series bandgap-like reflections in fact are attributed to considerable impedance mismatch caused by this ultra low Bloch impedance.
Feasibility Analysis of Novel Maglev EDM by Comparing with Conventional Micro EDM
Authors: Sisodiya Mangal Singh , Shukla Shashank , Bajpai Vivek 
Published: October 2021

Abstract
The Micro EDM is the most suitable machining process in the miniaturization of products at present era. The attainment of close precision is the primary obligation in miniaturization or micromachining. Especially in machining of hard-to-cut materials like titanium alloy. Such alloys are applied in several cutting-edge high-quit applications due to extraordinary properties. Current work presents an exploratory analysis of novel Maglev EDM while machining of commercially used pure titanium alloy (CP-TI). The work outlook is to establish the viability of novel Maglev EDM by comparing the with similar range of micro EDM. The pure DC power is employed with the conjunction of Maglev lucidity to refine the shortcomings of the conventional micro EDM. The novel technology deals with prime concerns of conventional micro EDM and cracked deficiencies like delay response of mechanical actuator and a servo mechanism. The Novel technology uses the logical arrangement of permanent and electromagnet to dealt with inadequacies like short-circuiting and arcing. The results of state of art novel technology proclaimed the improved Material removal rate (MRR), which are lying in the average range of 76.6 µgm/min, whereas the specific energy and surface roughness are observed 33.4 Joule/ microgram and 4.3 µm respectively.
A Black Hole-Aided Deep-Helix Channel Model for DNA
Authors: Sarker Md. Abdul Latif , Kader Md. Fazlul , Sarker Md. Mostafa Kamal , Lee Moon Ho , Dong  
Published: October 2021

Abstract

In this article, we present a black-hole-aided deep-helix (bh-dh) channel model to enhance information bound and mitigate a multiple-helix directional issue in Deoxyribonucleic acid (DNA) communications. The recent observations of DNA do not match with Shannon bound due to their multiple-helix directional issue. Hence, we propose a bh-dh channel model in this paper. The proposed bh-dh channel model follows a similar fashion of DNA and enriches the earlier DNA observations as well as achieving a composite like information bound. To do successfully the proposed bh-dh channel model, we first define a black-hole-aided Bernoulli-process and then consider a symmetric bh-dh channel model. After that, the geometric and graphical insight shows the resemblance of the proposed bh-dh channel model in DNA and Galaxy layout. In our exploration, the proposed bh-dh symmetric channel geometrically sketches a deep-pair-ellipse when a deep-pair information bit or digit is distributed in the proposed channel. Furthermore, the proposed channel graphically shapes as a beautiful circulant ring. The ring contains a central-hole, which looks like a central-black-hole of a Galaxy. The coordinates of the inner-ellipses denote a deep-double helix, and the coordinates of the outer-ellipses sketch a deep-parallel strand. Finally, the proposed bh-dh symmetric channel significantly outperforms the traditional binary-symmetric channel and is verified by computer simulations in terms of Shannon entropy and capacity bound.
A systematic review of the use of simulated client method in examining medicine price
Authors: Lee Kah Seng , Kassab Yaman Walid , Taha Nur Akmar , Zainal Zainol Akbar 
Published: October 2021

Abstract
Purpose: This study was aimed to systematically identify and review studies conducted using simulated client/patients that examine medicine selling prices in healthcare settings, including pharmacies, clinics, and hospitals. The quoted drug pricing information, including the dispensing fees and healthcare financing system of the countries involved, were also compared.Design/methodology/approach: We searched Pubmed, OVID, Scopus, Ebsco (Medline) and ProQuest for papers reporting on simulated patient studies in various medical settings, specifying drug or pharmaceutical pricings. Randomized controlled trials, non-randomized controlled trials, longitudinal studies, cohort or case–control studies, and descriptive studies were included. The quality of included studies was checked using a validated tool.Findings: A total of 19 papers from 13 countries from all regions of the world were included in this systematic review. Various ,edical conditions that were presented by the simulated patients, such as cancer, back pain, insomnia and diarrhoea. Information on certain prescription and non-prescription drugs such as buprenorphine, methadone and antibiotics were reported in four studies. Most healthcare facilities adhere to prescribed selling prices, according to the medicine selling price trend examined in this study. Only around half of the research found that medicine prices were higher than the typical selling price. Some countries that practise price control policies were found to have higher drug selling prices than those that do not.Originality/value: This review, for the first time, revealed that simulated patient method is a practical solution to obtain pharmaceutical price information from healthcare provider and most healthcare facilities adhere to the recommended selling prices.
Integrative analyses of biomarkers and pathways for heart failure
Authors: Fan Shaowei , Hu Yuanhui 
Published: October 2021

Abstract
Background: Heart failure (HF) is the most common potential cause of death, causing a huge health and economic burden all over the world. So far, some impressive progress has been made in the study of pathogenesis. However, the underlying molecular mechanisms leading to this disease remain to be fully elucidated. Methods: The microarray data sets of GSE76701, GSE21610 and GSE8331 were retrieved from the gene expression comprehensive database (GEO). After merging all microarray data and adjusting batch effects, differentially expressed genes (DEG) were determined. Functional enrichment analysis was performed based on Gene Ontology (GO) resources, Kyoto Encyclopedia of Genes and Genomes (KEGG) resources, gene set enrichment analysis (GSEA), response pathway database and Disease Ontology (DO). Protein protein interaction (PPI) network was constructed using string database. Combined with the above important bioinformatics information, the potential key genes were selected. The comparative toxicological genomics database (CTD) is used to explore the interaction between potential key genes and HF. Results: We identified 38 patients with heart failure and 16 normal controls. There were 315 DEGs among HF samples, including 278 up-regulated genes and 37 down-regulated genes. Pathway enrichment analysis showed that most DEGs were significantly enriched in BMP signal pathway, transmembrane receptor protein serine / threonine kinase signal pathway, extracellular matrix, basement membrane, glycosaminoglycan binding, sulfur compound binding and so on. Similarly, GSEA enrichment analysis showed that DEGs were mainly enriched in extracellular matrix and extracellular matrix related proteins. BBS9, CHRD, BMP4, MYH6, NPPA and CCL5 are central genes in PPI networks and modules. Conclusions: the enrichment pathway of DEGs and go ontology may reveal the molecular mechanism of HF. Among them, target genes EIF1AY, RPS4Y1, USP9Y, KDM5D, DDX3Y, NPPA, HBB, TSIX, LOC28556 and XIST are expected to become new targets for heart failure. Our findings provide potential biomarkers or therapeutic targets for the further study of heart failure and contribute to the development of advanced prediction, diagnosis and treatment strategies.
COVID-19 induces neuroinflammation and loss of hippocampal neurogenesis
Authors: Klein Robyn , Soung Allison , Sissoko Cheick , Nordvig Anna , Canoll Peter , Mariani Madeline , Jiang Xiaoping , Bricker Traci , Goldman James , Rosoklija Gorazd , Arango Victoria , Underwood Mark , Mann J. John , Boon Adrianus , Dowrk Andrew , Boldrini Maura 
Published: October 2021

Abstract
Infection with the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is associated with onset of neurological and psychiatric symptoms during and after the acute phase of illness1-4. Acute SARS-CoV-2 disease (COVID-19) presents with deficits of memory, attention, movement coordination, and mood. The mechanisms of these central nervous system symptoms remain largely unknown.In an established hamster model of intranasal infection with SARS-CoV-25, and patients deceased from COVID-19, we report a lack of viral neuroinvasion despite aberrant BBB permeability, microglial activation, and brain expression of interleukin (IL)-1β and IL-6, especially within the hippocampus and the inferior olivary nucleus of the medulla, when compared with non-COVID control hamsters and humans who died from other infections, cardiovascular disease, uremia or trauma. In the hippocampus dentate gyrus of both COVID-19 hamsters and humans, fewer cells expressed doublecortin, a marker of neuroblasts and immature neurons.Despite absence of viral neurotropism, we find SARS-CoV-2-induced inflammation, and hypoxia in humans, affect brain regions essential for fine motor function, learning, memory, and emotional responses, and result in loss of adult hippocampal neurogenesis. Neuroinflammation could affect cognition and behaviour via disruption of brain vasculature integrity, neurotransmission, and neurogenesis, acute effects that may persist in COVID-19 survivors with long-COVID symptoms.
Geometric Mediator Structures and Force Constants
Authors: koehler dale 
Published: October 2021

Abstract
It is shown in the present work that the distorted-space model of matter can describe conventional force-constants and transition-mediator structures. The distorted-geometry structures exhibit non-Newtonian features wherein the hole or core-region fields of the structures are energetically-repulsive (negative pressure), do not behave functionally in an r-4 manner and terminate at zero at the radial origin (no singularity). Near the core of the distortion the magnetic fields dominate the energy-densities of the structures thereby departing from classical particle-structure descriptions. Black-body radiation-emission and structural modeling leads to a description of transition dynamics and photonic entities.
Potential Habitat and Carrying Capacity of Endangered San Joaquin Kit Foxes in an Urban Environment: Implications for Conservation and Recovery
Authors: Cypher Brian , Deatherage Nicole A. , Westall Tory L. , Kelly Erica C. , Phillips Scott E. 
Published: October 2021

Abstract
The San Joaquin kit fox (Vulpes macrotis mutica; SJKF) is federally endangered due to profound habitat loss. A population of SJKF occurs in the urban environment in the city of Bakersfield, California, and may be important for SJKF conservation. We conducted a systematic survey using automated cameras stations and occupancy analysis to identify suitable habitat for SJKF in Bakersfield and to estimate a conceptual carrying capacity in the urban environment. We identified high, medium, and low suitability habitat totaling 121 km2, 196 km2, and 40 km2, respectively. Based on a mean home range size of 0.78 km2 and an assumption of two adults in high suitability home ranges and one adult in medium suitability ranges, we estimated the adult carrying capacity in Bakersfield to be 561 foxes. This estimate seems plausible as the number of adult SJKF in Bakersfield was estimated to be 381 in 2015, and the number of foxes already had been reduced by a sarcoptic mange epidemic that began two years earlier. A carrying capacity of 561 adults would increase the estimated range-wide carrying capacity by as much as 38%. Density estimates derived for the urban SJKF population based on the carrying capacity (1.57/km2) and home range size (2.56/km2) were higher than estimates for foxes in natural habitats. The urban SJKF population in Bakersfield is substantial and therefore could contribute significantly to conservation and recovery efforts for SJKF. Given our results, a potential conservation strategy may be to encourage or even establish additional urban SJKF populations.
Urethrocutaneous Fistula Following VMMC: A Case Series from March 2013 to October 2019 in ZAZIC’s Voluntary Medical Male Circumcision Program in Zimbabwe
Authors: Murenje Vernon , Victor Omollo , Paidemoyo Gonouya , Joseph Hove , Tinashe Munyaradzi , Phiona Marongwe , Mufuta Tshimanga , Vuyelwa Chitimbire , Sino Xaba , John Mandisarisa , Shirish Balachandra , Batsirai Makunike-Chikwinya , Marrianne Holec , Mangwiro Tonderayi , Scott Barnhart , Caryl Feldacker 
Published: October 2021

Abstract
Background: Urethrocutaneous fistula (subsequently, fistula) is a rare adverse event (AE) in voluntary medical male circumcision (VMMC) programs. Global fistula rates of 0.19 and 0.28 per 100,000 VMMCs were reported. Management of fistula can be complex and requires expert management. We describe seven cases of fistula in our large-scale VMMC program in Zimbabwe. We present fistula rates; provide an overview of initial management, surgical interventions, and patient outcomes; discuss causes; and suggest future prevention efforts. Results: Case details are presented on fistulas identified between March 2013 and October 2019. Among the seven fistula clients, ages ranged from 10-22 years; 6 cases were among boys under 15 years of age. All clients received surgical VMMC by trained providers in an outreach setting. Clients presented with fistulae 2 to 42 days after VMMC. Secondary infection was identified in 6 of 7 cases. Six cases were managed through surgical repair. The number of repair attempts ranged from 1 to 10. One case healed spontaneously with conservative management. Fistula rates are presented as cases/100,000 VMMCs.Conclusion: Fistula is an uncommon but severe AE that requires clinical expertise for successful management and repair. High-quality AE surveillance should identify fistula promptly and include consultation with experienced urologists. Strengthening provider surgical skills and establishment of standard protocols for fistula management would aid future prevention efforts in VMMC programs.