bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Association Between SYNTAX Score and Long-term Outcomes in Patients With Unstable Angina Pectoris: a Single-center Retrospective Study
Authors: Xu Min , Li Hong-Wei , Chen Hui 
Published: May 2021

Abstract
Background: SYNTAX Score affects clinical outcomes in early studies, whether SYNTAX Score could predict long-term outcomes in patients with unstable angina pectoris (UAP) in the era of new generation drug-eluting stent was unclear, and differences by SYNTAX Score in long-term outcomes between the medical therapy and percutaneous coronary intervention (PCI) in UAP patients were not well known.Methods：In this single-center retrospective study, a total of 2,364 patients with UAP from January 2014 to June 2017 at Beijing Friendship Hospital were enrolled. The primary endpoint was a composite of major adverse cardiovascular events (MACE) , including all-cause death, cardiac death, nonfatal myocardial infarction (MI) and stroke after at least 2 year from discharge. Results：In this study, 1,695 patients had low SYNTAX score (<22) , 432 patients had medium SYNTAX score (22-32), and 237 patients had high SYNTAX score (≥33), and 1,018 received medical therapy, 1,346 patients underwent PCI. Long-term MACE occurred in 95 patients during 3.38 ± 0.99 years follow up. Cox multivariate regression analysis showed advanced age, diabetes mellitus, heart failure, chronic kidney disease (CKD) and high SYNTAX score were independent predictors for MACE in the medical therapy group (P < 0.05), while, heart failure and CKD were predictors of MACE in PCI group. Compared to medical therapy group, PCI group showed lower MACE and cardiac death in patients with high SYNTAX score (≥33) (7.4% vs. 16.7%, P = 0.048; 3.7% vs 14.6%, P = 0.004), but no reduction in patients with low- and medium SYNTAX score.Conclusions：High SYNTAX score could predict long-term MACE for UAP patients with medical therapy, but not for patients undergoing PCI. Compared to medical therapy, PCI could significantly reduced long-term MACE and cardiac death for patients with high SYNTAX score.
Spin excitations in metallic kagome lattice FeSn and CoSn
Authors: Xie Yaofeng , Chen Lebing , Chen Tong , Wang Qi , Yin Qiangwei , Stewart J. , Stone Matthew , Daemen Luke , Feng Erxi , Cao Huibo , Lei Hechang , Yin Zhiping , MacDonald Allan , Dai Pengcheng 
Published: May 2021

Abstract
In two-dimensional (2D) metallic kagome lattice materials, destructive interference of electronic hopping pathways around the kagome bracket can produce nearly localized electrons, and thus electronic bands that are flat in momentum space. When ferromagnetic order breaks the degeneracy of the electronic bands and splits them into the spin-up majority and spin-down minority electronic bands, quasiparticle excitations between the spin-up and spin-down flat bands should form a narrow localized spin-excitation Stoner continuum coexisting with well-defined spin waves in the long wavelengths. Here we report inelastic neutron scattering studies of spin excitations in 2D metallic Kagome lattice antiferromagnetic FeSn and paramagnetic CoSn, where angle resolved photoemission spectroscopy experiments found spin-polarized and nonpolarized flat bands, respectively, below the Fermi level. Although our initial measurements on FeSn indeed reveal well-defined spin waves extending well above 140 meV coexisting with a flat excitation at 170 meV, subsequent experiments on CoSn indicate that the flat mode actually arises mostly from hydrocarbon scattering of the CYTOP-M commonly used to glue the samples to aluminum holder. Therefore, our results established the evolution of spin excitations in FeSn and CoSn, and identified an anomalous flat mode that has been overlooked by the neutron scattering community for the past 20 years.
Mechanistic Insights into Dideoxygenation in Gentamicin Biosynthesis
Authors: Li Sicong , Bury Priscila , Huang Fanglu , Guo Junhong , Sun Guo , Reva Anna , Huang Chuan , Jian Xinyun , Li Yuan , Zhou Jiahai , Deng Zixin , Leeper Finian , Leadlay Peter , Dias Marcio , Sun Yuhui 
Published: May 2021

Abstract
Gentamicin is an important aminoglycoside antibiotic used for treatment of infections caused by Gram-negative bacteria. Although most of the biosynthetic pathway of gentamicin has been elucidated, a remaining intriguing question is how the intermediates JI-20A and JI-20B undergo a dideoxygenation to form gentamicin C complex. Here we show that the dideoxygenation process starts with GenP-catalyzed phosphorylation of JI-20A and JI-20Ba. The phosphorylated products are converted to C1a and C2a by concerted actions of two PLP (pyridoxal 5'-phosphate)-dependent enzymes: elimination of water and then phosphate by GenB3 and double bond migration by GenB4. Each of these reactions liberates an imine which hydrolyses to a ketone or aldehyde and is then re-aminated by GenB3 using an amino donor. Crystal structures of GenB3 and GenB4 have guided site-directed mutagenesis to reveal crucial residues for the enzymes' functions. We propose catalytic mechanisms for GenB3 and GenB4, which shed new light on the already unrivalled catalytic versatility of PLP-dependent enzymes.
Feasibility Study on Machining Super-dimensioned Aviation Deep-hole Ti6Al4V Part With Axial Ultrasonic Vibration-assisted Boring
Authors: Sui He , Zhang Lifeng , Wang Shuang , Gu Zhaojun 
Published: May 2021

Abstract
Deep-holes are typical super-dimensioned parts of aircraft structures, with associated machining technology which is recognized as challenging due to the difficult-to-machine material, the narrow and enclosed machining environment, and the weak rigidity and large deformation of the boring bar. Axial ultrasonic vibration-assisted cutting has been proved to greatly enhance machining performance, especially in the precision machining of aviation alloy. This paper focuses on the machining of a super-dimensioned titanium alloy Ti6Al4V aviation deep-hole part (aspect ratio exceeding 20) with the axial ultrasonic vibration-assisted boring (AUVB) method. First, the kinetics of the AUVB process is analyzed and a retrospective of its separation cutting feature is provided. Subsequently, a multi-step cantilever beam model of the boring bar is established to analyze its static rigidity and dynamic stability. The aperture error of bored hole is deduced, and it is found to be mainly determined by the diameter of the basic hole, the cutting depth, the boring force and the length, diameter, elastic modulus, and rotational inertia of each step. Size coefficient, rather than aspect ratio, is then put forward to represent the static rigidity of the boring bar, which is proportional to the third power of the diameter and inversely proportional to the fourth power of the diameter. In addition, two different vibration cases, namely modal-coupling vibration and regenerative vibration are considered for dynamic stability analysis. The actual dynamic rigidity of AUVB in both of these cases is larger than that of conventional boring (CB), resulting from the lower actual boing force and the larger actual cutting depth. Next, the morphology of bored surface is analyzed, and the geometric height of peaks formed by AUVB and CB are calculated. Phase shift φ= π is suggested for obtaining a better surface in AUVB. Finally, the feasibility of AUVB on machining a super-dimensioned titanium alloy Ti6Al4V aviation deep-hole part with three different sizes of boring bar (size coefficients are 7473, 4076 and 2718, respectively) is verified through systematic experiments, and compared with CB. Results demonstrate that AUVB has obvious advantages in reducing the boring force, improving boring accuracy, suppressing vibration and promoting surface quality. Furthermore, the aperture error decreases to 50% and vibration amplitudes decrease to only 20%-25%. The overall surface roughness of the deep-hole part stays below Ra=0.8μm with rotational speeds of 60r/min and 80r/min, and the surface residual stress state is transferred from the tensile state to a compressive one. As a result, not only AUVB can provide better boring accuracy and surface finish, but it also can enhance the surface fatigue properties.
Ceria Nanoparticles Ameliorate Renal Fibrosis by Modulating the Balance Between Oxidative Phosphorylation and Aerobic Glycolysis
Authors: mengling Wang , feng Zeng , fengling Ning , yinhang Wang , jiaqi He , shilin Zhou , cong Li , dongliang Zhang , ping Hu , hong Xin , xudong Xu , xuemei Zhang 
Published: May 2021

Abstract
Background and aims: Renal fibrosis is the common outcome in all progressive forms of chronic kidney disease. Unfortunately, the pathogenesis of the renal fibrosis remains largely unexplored, among which metabolic reprogramming plays an extremely crucial role in the evolution of renal fibrosis. Ceria nanoparticles have strong ROS scavenging and anti-inflammatory activity. The present study aims to determine whether CeNPPEG has therapeutic value for renal fibrosis.Methods: Ceria nanoparticles and Ceria nanoparticles-PEG were synthesized according to previously reported procedures with slight modifications. For the in vivo studies, unilateral ureteral obstructive -induced kidney fibrosis was chosen. Through cellular studies, TGF-β1-induced epithelial-to-mesenchymal transition in HK-2 cells of enhanced glycolysis was initially utilized to report the associations among Ceria nanoparticles-PEG, oxidative phosphorylation, glycolysis and renal fibrosis.Results: In this study, Ceria nanoparticles were developed and the Ceria nanoparticles-PEG treatment significantly ameliorated renal fibrosis in unilateral ureteral obstructive mice as well as protected HK-2 cells from transforming growth factor beta1 -induced epithelial-to-mesenchymal transition process. Furthermore, we found that Ceria nanoparticles-PEG suppressed hexokinase 1and hexokinase 2 to block the dysregulated metabolic status, in which damaged epithelial cells take glycolytic metabolism as priority to oxidative phosphorylation, exerting the protective function in the progress of renal fibrosis.Conclusions: The application of Ceria nanoparticles-PEG in this study is attempted to provide another therapeutic option on renal fibrosis.
Sofosbuvir Is the Major Contributor to Elevation of Blood Cholesterol and Low-density Lipoprotein During HCV Treatment With DAAs
Authors: Wang Yi-Kai , Lee Wei-Ping , Wang Ying-Wen , Lu Chia-Ling , Huang Yi-Hsiang , Hou Ming-Chih , Chang Yuh-Lih , Lan Keng-Hsin 
Published: May 2021

Abstract
Background and aims: Worsened lipid profiles were observed in chronic hepatitis C (CHC) patients during direct-acting antivirals (DAAs) treatment, among which combination drugs confounded the effect of individual ingredient on lipid. Tenofovir alafenamide (TAF) also worsened lipid profiles in HIV patients. Structural similarity between sofosbuvir (SOF) and TAF prompted us to investigate rapid increase in total cholesterol (TC) and low-density lipoprotein (LDL) in CHC patients treated with SOF-based DAAs.Methods: A retrospective study was performed to analyze 487 CHC patients receiving DAAs with SVR12. Laboratory data were analyzed by logistic regression to determine relative risks of TC and LDL of SOF-based regimens over non-SOF-based regimens. Week 4 to baseline ratios of serum TC or LDL between regimens were compared by Mann-Whitney's test.Results: 487 patients were treated with Harvoni® (SOF-based, 206 patients), Epclusa® (SOF-based, 124 patients), Maviret® (non-SOF-based, 122 patients), or Zepatier® (non-SOF-based, 35 patients). At week 4 during drug treatment, Harvoni®, Epclusa®, and Maviret® induced statistically significant elevation of TC and LDL, but Zepatier® did not. SOF-based regimens had 2.72-fold higher relative risk (RR) causing 10% elevation of TC (95% CI: 1.84-4.02, p < 0.001) and 2.04-fold higher RR causing 10% elevation of LDL (95% CI:1.39-3.01, p < 0.001) than non-SOF-based DAAs. Conclusion: SOF-based DAAs were associated with significantly larger amplitude of increases in TC and LDL than non-SOFO-based regimens during the initial 4 weeks of treatment, but the amplitude was not sustained to SVR12.
Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea
Authors: Pang Yu , Zhang Yage , Chen Mengru , Lu Wei , Chen Ming , Yan Yongliang , Lin Min , Zhang Wei , ZHOU Zhengfu 
Published: May 2021

Abstract
A bacterial lipase producer, designated SCS 2–3, was isolated from deep-sea sediment of the South China Sea. Phylogenetic analysis based on the 16S rRNA sequences revealed that strain SCS2-3 belonged to the genus Pseudomonas and had 98.56% similarity with P. xinjiangensis NRRL B-51270T that the closest relative strain. MLSA using four protein-coding genes (dnaK, gyrA, recA, and rpoB) showed strain SCS 2–3 to branch separately from its most closely related type strains. ANI and in silico DDH values between strain SCS 2–3 and the closely related type strains of Pseudomonas species were less than 81.51% and 23.80%, respectively, both below the species delineation threshold. Genome comparison showed that strain SCS 2–3 shared 1875 core gene families with other eight closely related type strains in Pseudomonas, and the number of strain-unique genes was 263. Through gene annotations, genes related to lipase was found in the isolates’ genomes. Furthermore, a combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SCS 2–3 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nanhaiensis sp. nov. is proposed. The type strain is SCS 2-3T (= GDMCC 1.2219T = JCM 34440T).
Liposuction in Cancer‐Related Lower Extremity Lymphedema: an Investigative Study for Clinical Applications
Authors: Xin Jianfeng , Sun Yuguang , Xia Song , Chang Kun , Li Yiyin , Dong Jian , Shen Wenbin 
Published: May 2021

Abstract
Background: Lymphedema is a progressive, noncurable condition consisting of increases in subcutaneous fat and interstitial fluid in the limbs and fibrosis during later stages. The disease most commonly affects the limbs because of injury to or removal of lymph nodes. The aim of this study was to investigate therapeutic effects of liposuction for cancer-related lower extremity lymphedema.Methods 62 patients with cancer-related lymphedema in unilateral lower extremity were recruited in this study, and all the patients underwent liposuction. The volume of hemorrhage and lipid, the operation time and the volume changes of affected extremity were compared by applying t-test, and the subjective evaluation of the patients were also assessed by applying Chi-square. Results Total lipid volume is (2539±1253.5) ml, and the hemorrhage volume is (828±311.8) ml during liposuction. For the comparison of objective indexes, (1) Comparison of percent volume difference before surgery, in-operation and 3-month follow-up was (5.5±12.2 Vs. 11.6±18.4 Vs. 43.2±23.7, P＜0.05) respectively. (2) Higher lipid volume and liposuction rate for female patients, with lower volume of hemorrhage. (3) Higher volume of hemorrhage in patients with erysipelas (4) Higher lipid volume and liposuction rate, with lower hemorrhage for stage II than stage III patients.Conclusions Liposuction is an effective therapy for cancer-related lower extremity lymphedema, and it is necessary to combine with other therapeutic methods to improve lymph circulation.
Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge
Authors: Domínguez Jorge , Aira Manuel , Crandall Keith A. , Pérez-Losada Marcos 
Published: May 2021

Abstract
Wastewater treatment plants produce hundreds of million tons of sewage sludge every year all over the world. Vermicomposting is well established worldwide and has been successful in processing sewage sludge, which can contribute to alleviate the severe environmental problems caused by their disposal. Here, we utilized 16S and ITS rRNA high-throughput sequencing to characterize bacterial and fungal community composition and structure during the gut- and cast-associated processes (GAP and CAP, respectively) of vermicomposting of sewage sludge. Bacterial and fungal community composition and diversity changed significantly during both vermicomposting processes. Most of the bacterial and fungal taxa in the sewage sludge were eliminated during vermicomposting, mainly through the GAP. Vermicomposting of sewage resulted in a stable and rich microbial community with potential biostimulant properties that may aid plant growth. Our results support the use of vermicompost derived from sewage sludge for sustainable agricultural practices.
Acute hemolytic transfusion reaction caused by anti-Coa
Authors: Covin R.B. , Evans K.S. , Olshock R. , Thompson H.W. 
Published: May 2021

Abstract
Coa is a high-frequency blood group antigen in the Colton blood group system expressed on red blood cells (RBCs) of approximately 99.8 percent of random persons. Anti-Coa has been reported to cause delayed hemolytic transfusion reactions, hemolytic disease of the newborn, and accelerated clearance of RBCs in vivo. Acute hemolytic transfusion reactions (AHTRs) have not previously been reported. A 58-year-old man was hospitalized for vascular surgery. Initial blood bank evaluation revealed anti-Fya. The patient received six units of RBCs during his initial hospitalization and developed anti-E. A subsequent sample was sent to the reference laboratory when all units of RBCs appeared incompatible. Additional studies, including alloadsorptions, revealed the presence of anti-E, anti-Fya, and an apparent warm autoantibody. One unit of least-incompatible RBCs was transfused during surgery. The patient had an increase in temperature. Hemoglobinuria and a decrease in hematocrit were also noted. Due to the clinical impression of an AHTR, the pre- and postreaction samples were reevaluated in the reference laboratory and demonstrated the presence of anti-Coa in both. Based on clinical and laboratory evaluation this patient appears to have had an AHTR due to anti-Coa. This is the first known reported case of an AHTR caused by anti-Coa.
Immunohematology 2001;17:45–49.
Serologic and molecular investigations of a chimera
Authors: Mifsud Nicole A. , Haddad Albert P. , EHart Cathie , Holdsworth Rhonda , Condon Jennifer A. , Swain Michael , Sparrow Rosemary L 
Published: May 2021

Abstract
A chimeric individual possesses two or more genetically distinct ceil populations. Although the chimerism may not be evident in all gene systems, various loci display greater numbers of alleles than genetically “normal” individ uals. The proposit’a was referred for further laboratory investigation due to a mixed-field ABO blood group reaction following routine antenatal testing. Various molecular (HLA class II, ABO genotyping, and 10 short tandem repeat [STR] microsatellites) and serologic (HLA class I and red cell blood groups) typing techniques were employed to investigate a number of polymorphic loci located on different chromosomes. Chimerism was identified in 8 out of the 1.4 chromosomes tested: chromosome 1 (Duffy), 6 (HLA class I and Iï), 9 (ABO), 11 (HUMTH01), 12 (HUMPLA2A1), 15 (HUMFES/FPS). 18 (Kidd) and 21 (D21S11). The proposita was determined to be a probable dispermic chimera, based on the results of the serology and molecular studies.
Precipitation of serum proteins by polyethylene glycol (PEG) in pretransfusion testing
Authors: Hoffer Jack , Koslosky William P. , Gloster Elizabeth S. , Dimaio Tberese M. , Reid Marion E. 
Published: May 2021

Abstract
Polyethylene glycol (PEG) is used as a potentiator of blood group antigen-antibody interactions. Although PEG is known to precipitate immunoglobulins, we could find no reports of this reagent entrapping red blood cells (RBCs) in irreversible clumps. The patient we describe here had hyperglobulineinia with a reversed albumin:giob- ulin ratio and a diffuse immunoglobulin peak on serum protein electrophoresis. During preparation of serologic tests, a precipitate formed that entrapped the RBCs when PEG was added. Rapid recognition of this phenomenon could prevent delay in the selection. of blood for transfusion by substituting PEG-indirect antiglobulin test (1AT) with another technique such as low-ionic-strength solution (LISS-IAT, and by increasing the number of washes prior to addition of the antigiobulin reagent.
Hemochromatosis, iron, and blood donation: a short review
Authors: Fields Angela C. , Grindon Alfred J. 
Published: May 2021

Abstract
Hereditary hemochromatosis (HIT), an autosomal recessive disease of iron overload, is one of the most common inherited diseases. The candidate gene (HFE) for HH lias been identified recently and a DNA- based test for the mutation is available. Treatment for HH patients with elevated iron stores include repeated phlebotomy. Left untreated, iron overload can lead to cirrhosis, organ failure, and a shortened life expectancy. In the past and present, blood collected for therapeutic purposes from patients with HH has been discarded. The aim of this article is to address whether blood collected from HH patients should be used for allogeneic transfusion in the future.
Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DVI antigen from those with other partial D antigens or weak D antigens
Authors: Reid M.E. , Halverson G.R. , Roubinet F. , Apoil P.A. , Blancher A. 
Published: May 2021

Abstract
Historically, red blood cells (RBCs) with partial D antigens have been defined serologically by their pattern of reactivity with polyclonal and monoclonal anti-D. Although numerous variants have been described in tests with well-characterized monoclonal anti-D, definition remains difficult to ascertain serologically. RBCs of known partial D type were tested with LOR-15C9 (a monoclonal anti-D) and commercial anti-D by the tube indirect antiglobulin test (IAT), by micro typing system IgG gel cards, and by immunoblotting. By IAT, LOR-15C9 reacted strongly with DIIIa, DIIIc, DVa, DVI, DVII, and DFR RBCs in addition to RBCs with common D antigens; weakly with DII, DNU, and DIIIb RBCs; and not at all with DIVa, DIVb, DBT, or R0
Har RBCs. Reactivity was variable (1+ to 4+), with RBCs classified as weak D (Du). As expected, the commercial anti-D agglutinated all D variants and weak D RBC samples by the IAT and by using IgG gel cards; however, the reactivity with DVI RBCs was weaker than with LOR- 15C9. By immunoblotting, LOR-15C9 detected a band with an apparent molecular mass of approximate M
r 30,000-34,000 in membranes prepared from D-positive, DIIIa, DIIIc, DVa, DVI, DVII, and DFR RBCs and an additional band of Mr 20,000-22,000 in membranes prepared from DVI RBCs. No band(s) was detected in membranes from DII, DNU, DIIIb, DIVa, DIVb, DBT, R0
Har, weak D, or D-negative samples. LOR-15C9 provides a useful tool to identify positively DVI samples and thereby differentiate this partial D from other D variants and from weak D samples.