bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana
Authors: Kashkan Ivan , Hrtyan Mónika , Retzer Katarzyna , Humpolíčková Jana , Jayasree Aswathy , Filepová Roberta , Vondráková Zuzana , Simon Sibu , Rombaut Debbie , Jacobs Thomas B. , Frilander Mikko J. , Hejátko Jan , Friml Jiří , Petrášek Jan , Růžička Kamil 
Published: October 2021

Summary

Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators.

Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b.

PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin‐mediated tropic responses.

Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin‐mediated plant development.
Systematic review and meta‐analysis: Prevalence of diabetes among patients with tuberculosis in China
Authors: Du Qianmei , Wang Leiting , Long Qian , Zhao Yong , Abdullah Abu S. 
Published: October 2021

AbstractObjectiveTuberculosis (TB) and diabetes mellitus (DM) are both significant public health problems in China. Results of previous studies on the prevalence of DM among TB patients in China are inconsistent. We conducted this systematic review and meta‐analysis to estimate the overall prevalence of DM among TB patients in China.MethodsWe systematically searched Excerpta Medica Database, PubMed, Global Health, Ovid MEDLINE(R), Chinese Biomedical Literature Service System, and China Online Journals −Health & Medicine and included all observational studies reporting prevalence of DM among TB patients in China. The Cochran's Q‐statistic and were used to test heterogeneity. Finally, a random‐effect model meta‐analysis was conducted to estimate the pooled prevalence of DM among TB patients in China using R studio.ResultsWe screened 7043 articles and identified 43 eligible studies. The pooled prevalence was 7.8% (95% prediction interval 1.6–30.5, = 99.2%). The highest prevalence was observed in Northeast China (21.9%) among four economic regions of China, followed by East Coast (8.3%), Western China (5.9%), with Central China having the lowest prevalence (5.1%). Higher prevalence was observed in urban (10.1%) than in rural (7.8%) areas, and in hospital‐based (9.0%) than in population‐based studies (6.9%).ConclusionsThis study suggests that the prevalence of DM among TB patients in China aligns with the overall DM prevalence among the public in China. Public health strategies to reduce the burden of TB‐DM comorbidity and inequity are needed.
Estimación de la incertidumbre en la calibración de esfigmomanómetros según OIML R16-1 desde una perspectiva de metrología legal
Authors: Ramírez-Barrera Andrés Felipe , Delgado-Trejos Edilson , Ramírez-Gómez Vanessa 
Published: October 2021

Objetivo: Estimar la incertidumbre en la calibración de esfigmomanómetros bajo la OIML R16-1 desde una perspectiva de metrología legal. Materiales y métodos: Se seleccionaron aleatoriamente cuatro (4) esfigmomanómetros de diferentes marcas con resolución de 2 mmHg para realizar la calibración, la cual consiste en realizar comparaciones directas entre el esfigmomanómetro y un manómetro patrón digital con resolución de 0,01 mmHg, generando presión por medio de una pinza neumática. La incertidumbre se estima usando la Guía para la Expresión de la Incertidumbre de Medida (GUM) y la recomendación internacional OIML R16-1, mediante dos series de mediciones, cada una con secuencia ascendente y descendente, para obtener en cada caso 4 mediciones con su error correspondiente. Resultados y discusión: Se obtiene la representación de la incertidumbre en sus expresiones tipo A, tipo B y expandida, por medio de un modelado físico y matemático no estocástico. De las series de mediciones obtenidas del esfigmomanómetro en los procesos de calibración, se analizan los aportes obtenidos de incertidumbre, donde se infiere que la incertidumbre predominante, en la mayoría de los casos, es por resolución del equipo, seguido del comportamiento del equipo en la prueba de repetibilidad e histéresis. Conclusiones: La estructura propuesta para la estimación de la incertidumbre sigue los lineamientos internacionales y puede usarse también en procesos de aprobación de modelo para un impacto final en la seguridad del paciente.
Numerical Soliton Solutions of Fractional Modified (2 + 1)-Dimensional Konopelchenko–Dubrovsky Equations in Plasma Physics
Authors: Saha Ray S. , Sagar B 
Published: October 2021

Abstract
In this paper, the time-fractional modified (2 + 1)-dimensional Konopelchenko–Dubrovsky equations have been solved numerically using the Kansa method, in which the multiquadrics is used as radial basis function. To achieve this, a numerical scheme based on finite difference and Kansa method has been proposed. The stability and convergence of the proposed time-discretized scheme are theoretically proven. Also, the solitary wave solutions have been obtained by using Kudryashov technique. The computed results are compared with the exact solutions as well as with the soliton solutions obtained by Kudryashov technique to show the accuracy of the proposed method.
Towards Robust Methodology in Large-Scale Urban Reconstruction
Authors: García-Moreno Angel-Iván 
Published: October 2021

Abstract
The digitization of geographic environments, such as cities and archaeological sites, is of priority interest to the scientific community due to its potential applications. But there are still several issues to address. A comprehensive methodology is presented to reconstruct urban environments using a mobile land platform. All the implemented stages are described, the process to merge several point clouds to build a large-scale map is described, as well as the texture extraction and generation of surfaces, being able to render large urban areas using a low density of points but without losing the details of the structures within the urban scenes. The proposal is evaluated using several metrics, for example, coverage and root-mean-square-error (RMSE). The results are compared against three methodologies reported in the literature, obtaining better results in the 2D/3D data fusion process and the generation of surfaces. Presented method has a low RMSE (0.79) compared to the other methods and a runtime of approximately 40 s to process each data set (point cloud, panoramic image, and inertial data). In general, the proposed methodology shows a more homogeneous density distribution without losing the details, that is, it conserves the spatial distribution of the points, but with fewer data.
Aspergillus Lateral Flow Assay with Digital Reader for the Diagnosis of COVID-19-Associated Pulmonary Aspergillosis (CAPA): a Multicenter Study
Authors: Autier Brice , Prattes Juergen , White P. Lewis , Valerio Maricela , Machado Marina , Price Jessica , Egger Matthias , Gangneux Jean-Pierre , Hoenigl Martin 
Published: October 2021

This multicenter study evaluated the IMMY
Aspergillus
Galactomannan Lateral Flow Assay (LFA) with automated reader for diagnosis of pulmonary aspergillosis in patients with COVID-19-associated acute respiratory failure (ARF) requiring intensive care unit (ICU) admission between 03/2020 and 04/2021. A total of 196 respiratory samples and 148 serum samples (
n
= 344) from 238 patients were retrospectively included, with a maximum of one of each sample type per patient.
Varying association of laboratory values with reference ranges and outcomes in critically ill patients: an analysis of data from five databases in four countries across Asia, Europe and North America
Authors: Xu Haoran , Agha-Mir-Salim Louis , O’Brien Zachary , Huang Dora C , Li Peiyao , Gómez Josep , Liu Xiaoli , Liu Tongbo , Yeung Wesley , Thoral Patrick , Elbers Paul , Zhang Zhengbo , Saera María Bodí , Celi Leo Anthony 
Published: October 2021

BackgroundDespite wide usage across all areas of medicine, it is uncertain how useful standard reference ranges of laboratory values are for critically ill patients.ObjectivesThe aim of this study is to assess the distributions of standard laboratory measurements in more than 330 selected intensive care units (ICUs) across the USA, Amsterdam, Beijing and Tarragona; compare differences and similarities across different geographical locations and evaluate how they may be associated with differences in length of stay (LOS) and mortality in the ICU.MethodsA multi-centre, retrospective, cross-sectional study of data from five databases for adult patients first admitted to an ICU between 2001 and 2019 was conducted. The included databases contained patient-level data regarding demographics, interventions, clinical outcomes and laboratory results. Kernel density estimation functions were applied to the distributions of laboratory tests, and the overlapping coefficient and Cohen standardised mean difference were used to quantify differences in these distributions.ResultsThe 259 382 patients studied across five databases in four countries showed a high degree of heterogeneity with regard to demographics, case mix, interventions and outcomes. A high level of divergence in the studied laboratory results (creatinine, haemoglobin, lactate, sodium) from the locally used reference ranges was observed, even when stratified by outcome.ConclusionStandardised reference ranges have limited relevance to ICU patients across a range of geographies. The development of context-specific reference ranges, especially as it relates to clinical outcomes like LOS and mortality, may be more useful to clinicians.
Migraine and tension-type headache among undergraduate medical, dental and pharmaceutical students of University of Aleppo: a cross-sectional study
Authors: Alkarrash Mohamad Shadi , Shashaa Mohammad Nour , Kitaz Mohammad Nour , Rhayim Roaa , Alhasan Mahmoud Mohamad , Alassadi Maya , Aldakhil Ahmad , Alkhamis Mohamad , Ajam Mohanad , Douba Mohamad , Banjah Bashar , Ismail Ahmad , Zazo Aya , Zazo Rama , Abdulwahab Majd , Alkhamis Ali , Arab Abdullah , Alameen Mohammad Homam , Farfouti Mohamed Taher 
Published: October 2021

IntroductionHeadache disorders are among the most common 10 causes of disability worldwide according to the global burden of disease survey 2010. Headache is also wildly common among universities students when compared with other populations. The purpose of this study is to assess headache prevalence among Aleppo University medical, dental and pharmaceutical undergraduate students.MethodsA questionnaire-based cross-sectional study was conducted among medical, dental and pharmaceutical students at Aleppo University, Syria. We determined the type of headache according to the International Classification of Headache Disorder-III. The total number of participants was 2068. A χ2test was used to evaluate the association between the categorical outcomes. P<0.05 was considered significant.ResultsOut of 2068 participants, 1604 (77.6%) were medical students, 205 (9.9%) were dental students and 259 (12.5%) were pharmaceutical students. The effect on daily activities was higher in chronic tension headache (96.7%) and migraine without aura (94.6%) than migraine with aura (91.3) and episodic tension headache (85.1%). Out of 1191 who had a headache, only 188 (15.9%) had a medical consultation.ConclusionsThere was no a statistically significant difference in prevalence of tension headache and migraine according to faculties. There was a statistically significant difference in patients with migraine according to academic year, living with family and smoking. The effect on daily activities was higher in chronic tension-type headache and migraine without aura. There is a significant lack of medical consultation among students and most of them took over the counter analgesics depending on personal choice.
Screening for SARS-CoV-2 infection in asymptomatic individuals using the Panbio COVID-19 antigen rapid test (Abbott) compared with RT-PCR: a prospective cohort study
Authors: Winkel Beatrice , Schram Emma , Gremmels Hendrik , Debast Sylvia , Schuurman Rob , Wensing Annemarie , Bonten Marc , Goedhart Edwin , Hofstra Marije 
Published: October 2021

BackgroundAntigen-based point-of-care tests for identification of SARS-CoV-2 may markedly enhance effectiveness of population-based controlling strategies. Previous studies have demonstrated >70% sensitivity and high specificity compared with reverse transcriptase real-time PCR (RT-PCR) in symptomatic individuals, but test performance for asymptomatic individuals is unknown.MethodsTest performance of the Panbio COVID-19 Ag Rapid Test (Abbott) was compared with RT-PCR in a longitudinal cohort study of asymptomatic football players and staff members of professional football clubs. Based on timing of symptoms and prior and subsequent test results, positive RT-PCR tests were categorised as presymptomatic, early or late infection, or persistent RNA shedding.Findings2425 tests were performed in 824 individuals, of which 52 (6.3%) were SARS-CoV-2 positive based on RT-PCR. There were 2406 paired sets from asymptomatic subjects for analysis. Sixteen Panbio tests were inconclusive, for which sensitivity analyses were performed (considering results as either positive or negative or being excluded). Sensitivity of Panbio for screening of asymptomatic individuals ranged from 80.0% (61.4–92.3) to 86.67% (69.2–96.2) and specificity from 99.53% (95% CI 99.2 to 99.8) to 100% (95% CI 99.8 to 100). Sensitivity of Panbio to detect subjects with presymptomatic/early infection (n=42) ranged from 81.82% (95% CI 67.3 to 91.8) to 90.91% (95% CI 78.3 to 97.5) with specificity always above 99%.InterpretationThe Panbio COVID-19 Ag rapid test identifies 81%–90% of presymptomatic and early asymptomatic SARS-CoV-2 infections with high specificity. This test may therefore be adopted in testing strategies such as targeted screening of specific populations where prevalence is low.
Efficacy of metamizole versus ibuprofen and a short educational intervention versus standard care in acute and subacute low back pain: a study protocol of a randomised, multicentre, factorial trial (EMISI trial)
Authors: Wertli Maria M , Flury Julian S , Streit Sven , Limacher Andreas , Schuler Vanessa , Ferrante Asha-Naima , Rimensberger Caroline , Haschke Manuel 
Published: October 2021

IntroductionLow back pain (LBP) is among the top three most common diseases worldwide, resulting in a life with pain-related disability. To date, no study has assessed the efficacy of metamizole (dipyrone), a non-opioid analgesic and antipyretic prodrug compared with the conventional non-steroidal anti-inflammatory drug ibuprofen, in patients with an acute LBP episode. Further, it is unclear, whether a short educational intervention is superior to usual care alone.ObjectivesThe objective of this study is to assess first, whether metamizole is non-inferior to ibuprofen in a new episode of acute or subacute LBP. Second, we aim to assess whether a short educational intervention including evidence-based patient information on the nature of LBP is superior to usual care alone.Methods and analysisAn investigator-initiated multicentre, randomised, double blind trial using a factorial design will be performed. A total of 120 participants with a new episode of LBP will be recruited from GP practices, outpatient clinics and from emergency departments, and randomised into four different treatment groups: ibuprofen alone, ibuprofen and short intervention, metamizole alone, metamizole and short intervention. The primary endpoint for the medical treatment will be change in pain assessed on an 11-point Numeric Rating Scale after 14 days. The primary outcome for the short intervention will be change in the Core Outcome Measures Index assessed after 42 days.Ethics, dissemination and fundingThis study has been approved by the responsible Ethics Board (Ethikkommission Bern/2018-01986) and the Swiss Agency for Therapeutic Products (Swissmedic/2019DR4002). Results will be published in open access policy peer-reviewed journals. The study is funded by the Swiss National Science Foundation (grant number 32 003B-179346).Trial registration numberNCT04111315
Using social marketing for the promotion of cognitive health: a scoping review protocol
Authors: Barbier Mathilde , Schulte Caroline , Kornadt Anna , Federspiel Carine , Steinmetz Jean-Paul , Vögele Claus 
Published: October 2021

IntroductionThe use of social marketing strategies to induce the promotion of cognitive health has received little attention in research. The objective of this scoping review is twofold: (i) to identify the social marketing strategies that have been used in recent years to initiate and maintain health-promoting behaviour; (ii) to advance research in this area to inform policy and practice on how to best make use of these strategies to promote cognitive health.Methods and analysisWe will use the five-stage methodological framework of Arksey and O’Malley. Articles in English published since 2010 will be searched in electronic databases (the Cochrane Library, DoPHER, the International Bibliography of the Social Sciences, PsycInfo, PubMed, ScienceDirect, Scopus). Quantitative and qualitative study designs as well as reviews will be considered. We will include those articles that report the design, implementation, outcomes and evaluation of programmes and interventions concerning social marketing and/or health promotion and/or promotion of cognitive health. Grey literature will not be searched. Two independent reviewers will assess in detail the abstracts and full text of selected citations against the inclusion criteria. A Preferred Reporting Items for Systematic Reviews and Meta-Analyses flowchart for Scoping Reviews will be used to illustrate the process of article selection. We will use a data extraction form, present the results through narrative synthesis and discuss them in relation to the scoping review research questions.Ethics and disseminationEthics approval is not required for conducting this scoping review. The results of the review will be the first step to advance a conceptual framework, which contributes to the development of interventions targeting the promotion of cognitive health. The results will be published in a peer-reviewed scientific journal. They will also be disseminated to key stakeholders in the field of the promotion of cognitive health.
Study protocol for a multicentre randomised controlled trial studying the effect of a music intervention on anxiety in adult critically ill patients (The RELACS trial)
Authors: Kakar Ellaha , Van Mol Margo , Jeekel Johannes , Gommers Diederik , van der Jagt Mathieu 
Published: October 2021

IntroductionAnxiety is common in critically ill patients and has likely become more prevalent in the recent decade due to the imperative of the recent Clinical Practice Guidelines for the Prevention and Management of Pain, Agitation/Sedation, Delirium, Immobility, and Sleep Disruption in Adult Patients (PADIS) to use low levels of sedation and strive for wakefulness. However, management of anxiety has not been included in the PADIS guidelines, and there is lack of evidence to treat it in spite of its growing importance. Administration of sedative and analgesic medication is often chosen to reduce anxiety, especially when associated with agitation. Sedatives are associated with prolonged mechanical ventilation, delirium and muscle wasting and are therefore preferably minimised. Previous studies have suggested positive effects of music interventions on anxiety in the critically ill. Therefore, we aim to study the effect of music intervention on anxiety in adult critically ill patients.Methods and designA multicentre randomised controlled trial was designed to study the effect of a music intervention on the level of anxiety experienced by adult patients admitted to the intensive care unit (ICU). One hundred and four patients will be included in three centres in the Netherlands. Patient recruitment started on 24-08-2020 and is ongoing in three hospitals. The primary outcome is self-reported anxiety measured on the visual analogue scale. Secondary outcomes include anxiety measured using the six-item State-Trait Anxiety Inventory, sleep quality, agitation and sedation level, medication requirement, pain, delirium, complications, time spend on mechanical ventilation, physical parameters and ICU memory and experience.Ethics and disseminationThe Medical Ethics Review Board of Erasmus MC University Medical Centre Rotterdam, The Netherlands, has approved this protocol. The study is being conducted in accordance with the Declaration of Helsinki. Results of this trial will be published in peer-reviewed scientific journals and conference presentations.Trial registration numberNCT04796389.
Unmet needs in occupational health: prevention and management of viral hepatitis in healthcare workers in Ho Chi Minh City, Vietnam: a mixed-methods study
Authors: Nguyen Tran , Pham Trang , Tang Hong K , Phan Loc , Mize Gary , Lee William M , Gish Robert , Trang Amy , Le Anh , Phan Hai T , Nguyen Binh T , Dao Doan Y 
Published: October 2021

ObjectivesVietnam is an endemic area for hepatitis B virus and hepatitis C virus infection (HBV-HCV), yet its largest city, Ho Chi Minh City (HCMC), has no comprehensive policy to educate, screen, treat and protect healthcare workers (HCWs) from viral hepatitis. We conducted a mixed-methods study to document HBV-HCV infection rates, risk factors, local barriers and opportunities for providing education, screening and medical care for HCWs.DesignThis mixed-methods study involved an HBV and HCV serological evaluation, knowledge, attitude and practice survey about viral hepatitis and many in-depth interviews. Descriptive statistics and thematic content analysis using inductive and deductive approaches were used.SettingHCMC, Vietnam.ParticipantsHCWs at risk of viral hepatitis exposure at three hospitals in HCMC.ResultsOf the 210 invited HCWs, 203 were enrolled. Of the 203 HCWs enrolled, 20 were hepatitis B surface antigen-positive, 1 was anti-hepatitis C antibody (anti-HCV Ab)-positive, 57 were anti-hepatitis B core Ab-positive and 152 had adequate anti-hepatitis B surface Ab (anti-HBs Ab) titre (≥10IU/mL). Only 50% of the infected HCWs reported always using gloves during a clinical activity involving handling of blood or bodily fluid. Approximately 50% of HCWs were still not vaccinated against HBV following 1 year of employment. In-depth interviews revealed two major concerns for most interviewees: the need for financial support for HBV-HCV screening and treatment in HCWs and the need for specific HBV-HCV guidelines to be independently developed.ConclusionsThe high HBV infection rate in HCWs coupled with inadequate preventive occupational practices among the population in HCMC highlight the urgent needs to establish formal policy and rigorous education, screening, vaccination and treatment programmes to protect HCWs from HBV acquisition or to manage those living with chronic HBV in Vietnam.