bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tracing cortical circuits in humans and non-human primates from high resolution connectomic, transcriptomic, and temporal dimensions
Authors: Charvet Christine J. , Ofori Kwadwo , Baucum Christine , Sun Jianli , Modrell Melinda S. , Hekmatyar Khan , Edlow Brian L. , van der Kouwe Andre J. 
Published: May 2021

AbstractThe neural circuits that support human cognition are a topic of enduring interest. Yet, the lack of tools available to map human brain circuits has precluded our ability to trace the human and non-human primate connectome. We harnessed high-resolution connectomic, anatomic, and transcriptomic data to investigate the evolution and development of frontal cortex circuitry. We applied machine learning to RNA sequencing data to find corresponding ages between humans and macaques and to compare the development of circuits across species. We transcriptionally defined neural circuits by testing for associations between gene expression and white matter maturation. We then considered transcriptional and structural growth to test whether frontal cortex circuit maturation is unusually extended in humans relative to other species. We also considered gene expression and high-resolution diffusion MR tractography of adult brains to test for cross-species variation in frontal cortex circuits. We found that frontal cortex circuitry development is extended in primates, and concomitant with an expansion in cortico-cortical pathways compared with mice in adulthood. Importantly, we found that these parameters varied relatively little across humans and studied primates. These data identify a surprising collection of conserved features in frontal cortex circuits across humans and Old World monkeys. Our work demonstrates that integrating transcriptional and connectomic data across temporal dimensions is a robust approach to trace the evolution of brain connectomics in primates.Significance StatementWe lack appropriate tools to visualize the human brain connectome. We develop new approaches to study connections in the human and non-human primate brains. The integration of transcription with structure offers an unprecedented opportunity to study circuitry evolution. Our integrative approach finds corresponding ages across species and transcriptionally defines neural circuits. We used this information to test for variation in circuit maturation across species and found a surprising constellation of similar features in frontal cortex neural circuits across humans and primates. Integrating across scales of biological organization expands the repertoire of tools available to study connections in primates, which opens new avenues to study connections in health and diseases of the human brain.
A non-coding A-to-T Kozak site change related to the transmissibility of Alpha, Delta and Omicron VOCs
Authors: Yang Jianing , Zhang Guoqing , Yu Dalang , Cao Ruifang , Wu Xiaoxian , Ling Yunchao , Pan Yi-Hsuan , Yi Chunyan , Sun Xiaoyu , Sun Bing , Zhang Yu , Zhao Guo-Ping , Li Yixue , Li Haipeng 
Published: May 2021

AbstractThree prevalent SARS-CoV-2 Variants of Concern (VOCs) were emerged and caused epidemic waves. It is essential to uncover the key genetic changes that cause the high transmissibility of VOCs. However, different viral mutations are generally tightly linked so traditional population genetic methods may not reliably detect beneficial mutation. In this study, we proposed a new pandemic-scale phylogenomic approach to detect mutations crucial to transmissibility. We analyzed 3,646,973 high-quality SARS-CoV-2 genomic sequences and the epidemiology metadata. Based on the sequential occurrence order of mutations and the instantaneously accelerated furcation rate, the analysis revealed that two non-coding mutations at the position of 28271 (g.a28271-/t) might be crucial for the high transmissibility of Alpha, Delta and Omicron VOCs. Both two mutations cause an A-to-T change at the core Kozak site of theNgene. The analysis also revealed that the non-coding mutations (g.a28271-/t) alone are unlikely to cause high viral transmissibility, indicating epistasis or multilocus interaction in viral transmissibility. A convergent evolutionary analysis revealed that g.a28271-/t, S:P681H/R and N:R203K/M occur independently in the three-VOC lineages, suggesting a potential interaction among these mutations. Therefore, this study unveils that non-synonymous and non-coding mutations could affect the transmissibility synergistically.
The Split-Belt Rimless Wheel
Authors: Butterfield Julia K. , Simha Surabhi N. , Donelan J. Maxwell , Collins Steven H. 
Published: May 2021

AbstractSplit-belt treadmill training, common in stroke rehabilitation and motor learning experiments, reveals a mechanism through which energy can be extracted from the environment. People can extract net positive work from a split-belt treadmill by lengthening their step onto the fast belt. To understand how leg angles and belt speed differences affect energy transfer between the treadmill and the person during split-belt walking, we simulated a split-belt rimless wheel that alternates rotating on fast and slow treadmill belts. We found that the split-belt rimless wheel can passively walk steadily forward under a range of conditions, extracting enough energy from the treadmill to overcome losses during collisions. The simulated wheel can tolerate both speed disturbances and ground height variability, and it can even capture enough energy to walk uphill. We also built a physical split-belt rimless wheel robot, demonstrating the feasibility of energy extraction during split-belt treadmill walking. In comparing the wheel solutions to human split-belt gait, we found that humans do not maximize positive work performed by the treadmill; costs associated with balance and free vertical moments likely limit adaptation. This study characterizes the mechanics and energetics of split-belt walking, demonstrating that energy capture through intermittent contacts with the two belts is possible when the belt speed difference is paired with an asymmetry in leg angles at step-to-step transitions. This study demonstrates a novel way of harnessing energy through individual rotations rather than continuous contact and offers a simple model framework for understanding human choices during split-belt walking.
The chromatin insulator CTCF regulates HPV18 transcript splicing and differentiation-dependent late gene expression
Authors: Ferguson Jack , Leon Karen Campos , Pentland Ieisha , Stockton Joanne , Günther Thomas , Beggs Andrew , Roberts Sally , Noyvert Boris , Parish Joanna L. 
Published: May 2021

ABSTRACTThe ubiquitous host protein, CCCTC-binding factor (CTCF), is an essential regulator of cellular transcription and functions to maintain epigenetic boundaries, stabilise chromatin loops and regulate splicing of alternative exons. We have previously demonstrated that CTCF binds to the E2 open reading frame (ORF) of human papillomavirus (HPV) 18 and functions to repress viral oncogene expression in undifferentiated keratinocytes by co-ordinating an epigenetically repressed chromatin loop within HPV episomes. Cellular differentiation, which is necessary for HPV life cycle completion disrupts CTCF-dependent chromatin looping of HPV18 episomes inducing enhanced activity of the HPV18 early promoter P105and increased viral oncogene expression.To further characterise CTCF function in HPV transcription control we utilised direct, long-read Nanopore RNA-sequencing which provides information on the structure and abundance of full-length transcripts. Nanopore analysis of primary human keratinocytes containing HPV18 episomes before and after synchronous differentiation allowed quantification of viral transcript species in these cultures, including the identification of low abundance novel transcripts. Comparison of transcripts produced in wild type HPV18 genome-containing cells to those identified in CTCF-binding deficient genome-containing cells (HPV18-ΔCTCF) identifies CTCF as a key regulator of differentiation-dependent late promoter activation, required for efficient E1^E4 and L1 protein expression. Furthermore, our data show that CTCF binding at the E2 ORF of HPV18 promotes usage of the downstream weak splice donor (SD) sites SD3165 and SD3284, to the dominant E4 splice acceptor site at nucleotide 3434. These findings demonstrate importance of CTCF-dependent transcription regulation at multiple stages of the HPV life cycle.IMPORTANCEOncogenic human papillomavirus (HPV) infection is the cause of a subset of epithelial cancers of the uterine cervix, other anogenital areas and the oropharynx. HPV infection is established in the basal cells of epithelia where a restricted programme of viral gene expression is required for replication and maintenance of the viral episome. Completion of the HPV life cycle is dependent on the maturation (differentiation) of infected cells which induces enhanced viral gene expression and induction of capsid production. We previously reported that the host cell transcriptional regulator, CTCF, is hijacked by HPV to control viral gene expression. In this study, we use long-read mRNA sequencing to quantitatively map the variety and abundance of HPV transcripts produced in early and late stages of the HPV life cycle and to dissect the function of CTCF in controlling HPV gene expression and transcript processing.
mir124-dependent tagging of glutamatergic synapses by synaptopodin controls non-uniform and input-specific homeostatic synaptic plasticity
Authors: Dubes Sandra , Soula Anaïs , Benquet Sébastien , Tessier Béatrice , Poujol Christel , Favereaux Alexandre , Thoumine Olivier , Letellier Mathieu 
Published: May 2021

SummaryHomeostatic synaptic plasticity (HSP) is a process by which neurons adjust synaptic strengths to compensate for various perturbations and which allows to stabilize neuronal activity. Yet, whether the highly diverse synapses harboring a neuron respond uniformly to a same perturbation is unclear and the underlying molecular determinants remain to be identified. Here, using patch-clamp recordings, immunolabeling and imaging approaches, we report that the ability of individual synapses to undergo HSP in response to activity-deprivation paradigms depends on the local expression of the spine apparatus related protein synaptopodin (SP) acting as a synaptic tag to promote AMPA receptor synaptic accumulation and spine growth. Gain and loss-of-function experiments indicate that this process relies on the local de-repression of SP translation by miR124 which supports both non-uniform and synapse-autonomous HSP induced by global or inputspecific activity deprivation, respectively. Our findings uncover an unexpected synaptic-tagging mechanism for HSP, whose molecular actors are intriguingly shared with Hebbian plasticity and linked to multiple neurological diseases.
Green project management from employees' perspective in Hong Kong's engineering and construction sectors
Authors: To Wai Ming , Lam K.H. 
Published: May 2021

PurposeThe study investigates employees’ perceptions of green project management in Hong Kong's engineering and construction sectors.Design/methodology/approachGreen project management attributes were identified and categorized in terms of organization and process aspects based on a literature review. A questionnaire was developed to collect responses from employees working in Hong Kong's engineering and construction sectors.FindingsRespondents perceived “using Design for Environment approach,” “training employees about green project management” and “recycling the used or excessive materials/components” as the most important attributes. Generally, females gave higher importance ratings than males to most attributes. Respondents who had higher education qualifications or held senior positions perceived green project management attributes as more important than their counterparts with lower education qualifications or in lower positions. Green project management was found to have four distinct factors: “Management Commitment,” “Green Technologies and Processes,” “Green Partnerships” and “External Communication.”Originality/valueThe study is one of the first empirical works on green project management in Hong Kong's engineering and construction sectors. It demonstrates that green project management should be characterized as a multidimensional concept.
A retrospective overview ofJournal of Enterprise Information Managementusing bibliometric analysis
Authors: Donthu Naveen , Kumar Badhotiya Gaurav , Kumar Satish , Soni Gunjan , Pandey Nitesh 
Published: May 2021

PurposeJournal of Enterprise Information Management(JEIM) is a leading journal that publishes studies on applied information management relevant to industry personals, academicians and researchers. This study uses bibliometric tools to present a retrospective analysis of the journal's outcomes.Design/methodology/approachThe authors applied bibliometric tools for analysing the impact, topic coverage, renowned authors with affiliation, citation, methodology and analysis of theJEIMcorpus. Additionally, they used bibliographic coupling to develop a graphical visualisation and analyse the journal's thematic evolution.FindingsWith 16 yearly articles,JEIMcontributed 656 research articles on various themes. The major themes that have come to define theJEIMover this time include information and systems, supply chain management, manufacturing resource planning, communication technologies and small- to medium-sized enterprises. Empirical methodology, quantitative techniques with descriptive analysis and regression methods are the most preferred. The article's primary research purpose shows the majority of theory-verifying articles. Co-authorship analysis reveals that the single-author trend is decreasing and the journal now has articles with international collaborations.Originality/valueThis study is the retrospective analysis of theJEIM, which is useful for aspiring contributors and the journal's editors.
The dichotomisation fallacy of public and private corruption and the quantification dilemma
Authors: Ekwueme Ejike 
Published: May 2021

Purpose
This paper aims to examine the concept of corruption and dirty money. Corruption is amorphous and lacks a congruent definition. It is mainly divided into public and private corruption. This divide, is unnecessary, given the fact that both cause incalculable damage to the markets and lager society. Globalisation has necessitated liberalisation and resulted in amalgamating both public and private ventures. This, as a result, has made it more difficult to stick to this. Pronouncements from International Chamber of Commerce (ICC) and the Law Commission’s attitude not to segregate between private and public bribery prior to the legislation of United Kingdom Bribery Act 2010, has added greater impetus to the debate. Attempts to quantify the amount of corruption and money laundering, has equally, hit a dead end. The figures being bandied about are all estimates or “guesstimates” that cannot stand the empirical test. As a result, the conjectures have strong potentials to continue for a longer time. The purpose of this paper is to bring to the fore the need to jettison the long-held perception that public and private corruption should be seen in different lights.

Design/methodology/approach
This paper relies substantially on both primary and secondary sources in the analysis.

Findings
Indicatively, the facts tilt towards the conclusion that it is impossible to actually ascertain the quantifiable amount of money that is involved in corruption and the money laundering process. It is an illusion.

Originality/value
The paper provides the platform that the time is ripe for both public and private corruption to be seen as the same thing, as they both unleash catastrophic consequences on society. The issues of globalisation and liberalisation make this inevitable.
Aircraft routing problem model for fractional fleets using fault prognostics
Authors: Pereira Barreto Eduardo Afonso , Abrahão Fernando Teixeira Mendes Teixeira Mendes , Olivares Loesch Vianna Wlamir 
Published: May 2021

PurposeThe objective of this work is to provide a novel aircraft allocation model for fractional business aviation. This model may provide decision-makers with alternative routing solutions that take into consideration preventive maintenance and failure prognostics information. The expected results are more efficient routing solutions when compared to conventional planning models, to help decision-makers improve operations and maintenance planning.Design/methodology/approachThe model is a mixed integer linear problem formulation addressing and considering preventive maintenance and failure prognostics for optimal operations. Numerical experiments were performed using both field and synthetic data to validate the proposed method. All instances are solved using branch, price and cut algorithms from open-source software.FindingsThe results obtained in this study show that the use of failure prognostics information in aircraft routing can provide improvements in overall planning. By choosing slightly longer flight legs, the flight cost will increase, but putting an aircraft with a higher risk of failure on a leg inbound to a maintenance base can reduce maintenance and overall operating cost.Originality/valueThe model and method provide decision-makers with routing solutions that consider new aspects of planning, not used in previous works, such as failure. Most of the literature focuses on solving routing problems for large commercial airlines. Considering that, few solutions are found in literature for fractional business operators, which have their own operational particularities, such as a company managing a fleet of aircraft belonging to multiple shareowners. In such operation, clients may not always fly in the aircraft that they are shareowners, but an aircraft from the fractional fleet of the same category. Here, the company managing the aircraft guarantees that an aircraft will be ready to attend client demands in minimum time. One of the major differences from other models of operation is the dynamic nature of its flight demands, thus requiring flexible and agile planning limiting the available time to find a routing solution.
Entrepreneurs' emotional intelligence as a factor explaining entrepreneurial psychological ownership and high-tech start-up growth
Authors: Yitshaki Ronit 
Published: May 2021

PurposeThe purpose of this study is to examine the role of emotional intelligence (EI) in explaining high-tech entrepreneurs' psychological ownership (PO) and, in turn, how their PO influences venture growth.Design/methodology/approachThis study is based on data collected from 106 high-tech Israeli entrepreneurs.FindingsThe findings indicate that using emotions for problem-solving is positively associated with PO sense of efficacy, and that appraisal of emotions of others is negatively associated with PO sense of territoriality. Moreover, PO sense of efficacy is positively associated with growth, whereas sense of territoriality is negatively associated with growth. Finally, sense of territoriality has a curvilinear effect on venture growth.Research limitations/implicationsUnderstanding of the antecedents of entrepreneurs' PO and its influence on firms' growth is scant. The findings contribute to the psychology of entrepreneurship and PO literature by showing the influence of affective-based processes on high-tech start-up growth.Practical implicationsThe findings of this study suggest the entrepreneurs' EI indirectly affects other affective behaviors such as PO, which in turn influence venture growth. It highlights the need of entrepreneur to regulate their emotions and manage the emotional- and cognitive-based processes for the sake of firms' growth.Originality/valueThe findings expand the knowledge pertaining to the psychology of entrepreneurship by showing how EI as an affective dimension is interrelated with affective-based process and entrepreneurial firms' growth.
Entrepreneurial competencies, entrepreneurial orientation, entrepreneurial network, government business support and SMEs performance. The moderating role of the external environment
Authors: Pulka Buba Musa , Ramli Azahari , Mohamad Armanurah 
Published: May 2021

PurposeThe purpose of this study is to examine the moderating influence of the external environment on the relationship between entrepreneurial competencies, entrepreneurial orientation, entrepreneurial network, government business support and SMEs performance. The objectives of the study are achieved using the resource-based view and dynamic capability theory.Design/methodology/approachThe survey method of research was used by personally administering questionnaires to the respondents. Multistage sampling techniques are used in selecting 470 SMEs owners/managers that participated in the survey. SPSS 24 and PLS-SEM 3.0 were used in the analysis of the data.FindingsIn the Nigerian context, the findings indicated that EC, EO and GBS directly influence the SMEs performance. Surprisingly, SMEs performance is not influenced by EN. Similarly, EE significantly moderated the relationship between EC, GBS and SMEs performance. On the contrary, EE does not have any moderating influence on the relationship between EO, EN and SMEs performance.Research limitations/implicationsThe study is limited to northeastern Nigeria. The study is limited to the EC, EO, EN GBS EE and SMEs performance and the use of cross-sectional data. The findings imply that SMEs owners/managers need a high level of entrepreneurial competencies and government business support to achieve a better performance especially in an external environment that is characterised by dynamism, diversity, complexity and hostility. Hence, providing support for both RBV and DCT.Practical implicationsThus, the study offers additional empirical evidence from Nigeria and also expands knowledge and understanding in this field. The findings offer owners/managers, government agencies, financial institutions and other stakeholders of SMEs strategies EC, EO, GBS and EE to achieve a better SMEs performance.Originality/valueThe conceptual framework of the study is unique, and the study was conducted in northeastern Nigeria which is grossly underrepresented in the literature. It also provided understanding on the moderating influence of EE on the framework.
Knowledge and attitudes toward genetically modified (GM) food among health sciences university students in Karachi, Pakistan
Authors: Amin Rafat , Khan Sadaf , Zeb Tehseen Fatima , Ali Shaukat , Baqai Neha , Baqai Maham , Shuja Shajiha 
Published: May 2021

Purpose
Genetically modified (GM) foods have global importance as they can significantly improve food security challenges. However, risks associated with the consumption of GM foods have affected consumers’ acceptance. This study aims to assess the student’s awareness regarding GM foods.

Design/methodology/approach
A cross-sectional survey-based study was conducted to explore the knowledge and attitude of health sciences university students toward GM foods. Students (n = 400) from different academic disciplines were participated in the study.

Findings
The majority of the university students heard about GM organisms and GM food (67.5% and 60.8%, respectively) and have correctly defined GM foods (48.2%). The mean percentage score of knowledge and attitude toward GM foods among university students was 36.0 ± 29.4% and 29.8 ± 23.1%, respectively. No significant difference was observed in knowledge and attitude between undergraduate and postgraduate students. A significant difference was found toward the acceptability of GM food in students from different health sciences disciplines (p = 0.006).

Originality/value
The findings indicate that educational programs are needed to improve the knowledge of students about GM food and food safety challenges.