bluuebunny/crossref_metadata_2025_split huggingface dataset:
How Much Time Do Midwives Spend on Antenatal Care? Assessment of Antenatal Care (ANC) in Six Districts/cities of West Java-indonesia
Authors: Dewi Evindiyah Prita , Sjaaf Amal Chalik , Boerman Baequni 
Published: August 2021

Abstract
Background: The government makes various policies to improve the health of pregnant women, which in turn has an impact on reducing maternal mortality and infant mortality. Antenatal care (ANC) is the most important strategy to improve the health of pregnant women. Distric/city governments must ensure the availability of antenatal services for their communities in primary health facilities (PHF). Midwives are the primary provider of antenatal care at PHF. This study describes the time required for midwives to provide standardized antenatal care.Methods: This study was a time study method, which analyzes the average time of ANC Services by midwives in PHF. The study observed fifty-eight midwives who gave ANC services to five hundred fifty-two pregnant women in six districts in West Java. The data collection was carried out in January-February 2019.Results: In this study, it was found that the time to ANC varied widely; for ANC-I, the average is 30.34 minutes (Min 25 minutes- Max 50 minutes; lower mean 56,7%), ANC-II 23.12 minutes (Min 17 minutes - Max 36 minutes; Lower mean 63,5%), and ANC-T 26.9 minutes (Min 23 minutes - Max 35 minutes; lower mean 53,2%). The order of service time is as follows: ANC-I, ANC-T and ANC-II.Conclusions: The time used for ANC is still significantly less than that recommended by the WHO for pregnant women's visits (40 minutes per visit). The reason will undoubtedly affect the quality of ANC services. It is hoped that District/City Administrative and Health Authorities will pay more attention to the duration time of ANC visits by developing a standard time of visit per patient or increasing the number of midwives in carrying out ANC services at that location.
Children with Strabismus and Amblyopia Presented Abnormal Spontaneous Brain Activities Measured Through Fractional Amplitude of Low-Frequency Fluctuation (fALFF)
Authors: Liu Li-Qi , Shi Yi-Dan , Liang Rong-Bin , Ge Qian-Min , Li Qiu-Yu , Pan Yi-Cong , Shu Hui-Ye , Zhang Li-Juan , Shao YI 
Published: August 2021

Abstract
Purpose

Based on fMRI technology, we explored whether children with strabismus and amblyopia (SA) showed significant alteration in fractional amplitude of low-frequency fluctuation (fALFF) values in specific brain regions compared with healthy controls, and whether this change could indicate the clinical manifestations and pathogenesis of children with strabismus to a certain extent.
Methods

23 children with strabismus amblyopia and same number matched healthy control was registered in the ophthalmology department of the First Affiliated Hospital of Nanchang University, and the whole brain was scanned by rs-fMRI. The fALFF value of each brain area was derived to explore whether there is a statistical difference in the two groups. Meanwhile, ROC curve was made in a view to evaluate whether this difference is of value as a diagnostic index. Finally, analyze whether the changes in the fALFF value of some specific brain regions are related to clinical manifestations
Results

report to HCs children with SA presented a decreased fALFF values in left temporal pole: the superior temporal gyrus, right middle temporal gyrus, right superior frontal gyrus, right supplementary motor area. Meanwhile, they also showed higher fALFF values in specific brain areas, which included left precentral gyrus, left inferior Parietal, left Precuneus.
Conclusion

Children with SA showed abnormal fALFF values in different brain regions. Most of these regions were allocated to the visual formation pathway. The eye movement-related pathway or other visual-related pathways, suggesting the pathological mechanism of the patient.
D-Dimer Level Significance for Deep Vein Thrombosis Screening in The Third Trimester : A Retrospective Study
Authors: Miyamoto Keisuke , Komatsu Hiroaki , Okawa Masayo , Iida Yuki , Osaku Daiken , Azuma Yukihiro , Tsuneto Takako , Harada Takashi , Taniguchi Fuminori , Harada Tasuku 
Published: August 2021

Abstract
BackgroundVenous thromboembolism often develops after surgery and childbirth, resulting in death in some cases. Although early deep vein thrombosis (DVT) detection can predict pulmonary thromboembolism, there is no early screening method for DVT in pregnant women. Lack of consensus regarding significance or setting and cut-off value interpretation of D-dimer levels further impedes venous thromboembolism screening in pregnant women.This study aimed to examine the utility of third-trimester serum D-dimer levels for screening test for DVT during pregnancy.to determine the frequency of asymptomatic DVT using lower-limb compression ultrasonography.MethodsThis single-center retrospective study included 497 pregnant women who underwent elective cesarean section at term in our hospital between January 2013 and December 2019. Serum D-dimer levels were preoperatively measured at 32–37 gestation weeks. The presence or absence of DVT in patients with serum D-dimer levels ≥ 3.0 µg/ml, the cut-off value, was examined using compression ultrasonography. In all patients, the presence or absence of clinical venous thrombosis (symptoms such as lower-limb pain, swelling, and heat sensation) was examined within 4 postoperative weeks.The Royal College of Obstetricians and Gynecologists Guideline 2015 was referred to determine risk factors for the onset of venous thrombosis during pregnancy. Among those, we examined the risk factors for DVT that result in high D-dimer levels during pregnancy.ResultsThe median age and body mass index were 35 (20–47) years and 21.2 (16.4–41.1) kg/m2, respectively. Further, the median gestational age and D-dimer levels were 37 weeks and 2.1 (0.2–16.0) µg/ml, respectively. Compression ultrasonography was performed on 135 (26.5%) patients with a D-dimer level ≥3.0 µg/ml, with none of the patients showing DVT. All patients were followed up for 4 postoperative weeks, with none presenting with venous thromboembolism. Multivariate analysis showed that hypertensive disorders of pregnancy is an independent risk factor for venous thromboembolism that causes high D-dimer levels (odds ratio: 2.48, 95% confidence interval: 1.05–6.50, P=0.04).ConclusionThere may be low utility in screening for DVT using D-dimer levels in the third trimester. Further, prepartum asymptomatic DVT has a low frequency, indicating the low utility of compression ultrasonography.Trial registrationInstitutional Review Board of Tottori University Hospital (IRB no. 20A149).
Interaction and transformation of metastable defects in intercalation materials
Authors: Gorobtsov Oleg , Hirsh Hayley , Zhang Minghao , Sheyfer Dina , Matson Stephanie , Weinstock Daniel , Bouck Ryan , Wang Ziyi , Cha Wonsuk , Maser Jorg , Harder Ross , Meng Ying Shirley , Singer Andrej 
Published: August 2021

Abstract
Non-equilibrium defects often dictate macroscopic functional properties of materials. In intercalation hosts, widely used in rechargeable batteries, high-dimensional defects largely define reversibility and kinetics. However, transient defects briefly appearing during ionic transport have been challenging to capture, limiting the understanding of their life cycle and impact. Here, we overcome this challenge and track operando the interaction and impact of metastable defects within NaxNi1-xMnyO2 intercalation hosts in a charging sodium-ion battery. Three-dimensional coherent X-ray imaging reveals transformation and self-healing of a metastable domain boundary, glissile dislocation loop, and stacking fault. A local strain gradient suggests a quantifiable difference in ion diffusion, coincident with the macroscopic change in diffusion coefficient. Analysis of the unexpected defect anisotropy highlights the importance of mesostructure, suggesting a possible control approach and disputing the rigidity of the framework layers. The shared nature of oxygen framework layers makes our results applicable to a wide range of intercalation materials.
REV7/FANCV Binds to CAMP and Promotes Homologous Recombination Repair
Authors: Li Feng , Sarangi Prabha , Feng Hanrong , Moreau Lisa , Nguyen Huy , Clairmont Connor , D'Andrea Alan 
Published: August 2021

Abstract
A critical determinant of DNA repair pathway choice is the HORMA protein REV7, a small abundant adaptor which binds to various DNA repair proteins through its C-terminal seatbelt domain. The REV7 seatbelt binds to the REV3 polymerase to form the Polymerase ζ complex, a positive regulator of translesion synthesis (TLS) repair. Alternatively, the REV7 seatbelt binds to SHLD3 in the Shieldin complex, a positive regulator of NHEJ repair. Recent studies have identified another novel REV7 seatbelt-binding protein, CAMP (Chromosome Alignment-Maintaining Phosphoprotein), though its role in DNA repair is unknown. Here, we show that the REV7-CAMP complex promotes homologous recombination (HR) repair by sequestering REV7 from the Shieldin complex. CAMP competes directly with the SHLD3 subunit of the Shieldin complex for a limited pool of C-REV7, thereby inhibiting the REV7-mediated recruitment of the SHLD2 and SHLD1 effector subunits to DNA double strand breaks. CAMP thereby channels DNA repair away from error-prone NHEJ and towards the competing error-free HR pathway. Similarly, CAMP competes with the REV3 component of the POL-Zeta complex, thereby reducing the level of mutagenic TLS repair. CAMP has a distinct function in promoting chromosome alignment which is independent of its REV7 binding activity. Importantly, in human tumors, CAMP overexpression promotes HR, confers PARP inhibitor resistance, and correlates with poor prognosis. Thus, by binding to either REV3, SHLD3, or CAMP through its seatbelt, the REV7 protein can promote either TLS repair, NHEJ repair, or HR repair respectively.
Gene Expression Analysis of Resistant and Susceptible Rice Cultivars to Sheath Blight after Inoculation with Rhizoctonia Solani
Authors: Yang Xiaohe , Gu Xin , Ding Junjie , Yao Liangliang , Gao Xuedong , Zhang Maoming , Meng Qingying , Wei Songhong , Fu Junfan 
Published: August 2021

Abstract
Background: Rice sheath blight, caused by Rhizoctonia solani Kühn (teleomorph: Thanatephorus cucumeris), is one of the most severe diseases in rice (Oryza sativa L.) worldwide. Studies on resistance genes and resistance mechanisms of rice sheath blight has mainly focused on indica rice. Rice sheath blight is a growing threat to rice production with the increasing planting area of japonica rice in Northeast China, and it is therefore essential to explore the mechanism of sheath blight resistance in this rice subspecies.Results: In this study, RNA-seq technology was used to analyse the gene expression changes of leaf sheath inoculation with R. solani at 12, 24, 36, 48, and 72 h after inoculation of the resistant cultivar ‘Shennong 9819’ and susceptible cultivar ‘Yueguang’. A total of 2275 differentially expressed genes (DEGs) were identified in our study. Gene Ontology (GO) annotation and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis of these DEGs showed that the metabolic pathways of the two rice cultivars were similar and approximately covered most of the biological processes after inoculation with R. solani. Conclusion: The main differences between the resistant and susceptible cultivars were the activity of DEGs in response to R. solani and the enrichment of metabolic pathways involved in these genes. Upon experiencing external stress, the genes in the leaf sheath of Shennong 9819 showed a fluctuating response, whereas the genes in the leaf sheath of Yueguang had a higher activity. The phenylalanine metabolism and plant hormone signal transduction play important roles in disease resistance. Their response in the leaf sheath of Shennong 9819 was faster than that of Yueguang, and they were highly enriched at 12 h after inoculation.
A Novel Model To Predict Endometriosis in Patients with Ovarian Cysts: A Retrospective Study.
Authors: Xiang Junmiao , Shen Wei , Liang Zongwen , Zhang Qiong , Duan Ping 
Published: August 2021

Abstract
ObjectiveTo develop a model that uses hematological indexes and clinical characteristics to help estimate the probability of endometriosis in patients with ovarian cysts.MethodsA retrospective study was conducted on 2242 patients who underwent surgery for benign ovarian cysts from January 2008 to November 2016. Variables included in the model were serum tumor markers, blood routine test, age, BMI, reproductive history, history of hysteroscopy, menstrual episodes. Logistic regression was used to construct a predictive model for endometriosis, Receiver Operating Characteristic curves and the areas under the curve was used to verify the model’s validities. Ten-fold cross-validation was primarily used as an internal validation to evaluate the prediction accuracies of the model. Normalized mean square errors (NMSE) was obtained to compare the reliability of different models.Results978 (43.6%) patients with endometriosis were included in the strudy. Univariate analysis showed that age, BMI, delivery, dysmenorrhea, menstrual cycle, duration of menstrual flow, history of hysteroscopy, CA-125 and CA-19-9 (P < 0.001) are associated with endometriosis. The area under the receiver operating characteristic curve for the model with CA-125 alone was 0.888, with a sensitivity of 81.6% and specificity of 83.5%. After adjustment for other multiple covariates, including age, mature delivery, irregular menstruation, dysmenorrhea, menstrual period, history of hysteroscopy, CEA, CA-19-9, monocyte count, platelet count, the model obtained had an AUC of 0.916, with a sensitivity of 0.849 and specificity of 0.864.ConclusionsThe diagnostic prediction model can be used as a framework for potential improvement in diagnosis of endometriosis in patient with ovarian cyst.
A Novel Homozygous USP53 Splicing Variant Disrupting the Gene Function that Causes Cholestasis Phenotype and Review of the Literature
Authors: GEZDIRICI ALPER , ŞENGÜL ÖZLEM KALAYCIK , DOĞAN MUSTAFA , ÖZGÜVEN BANU YILMAZ , AKBULUT EKREM 
Published: August 2021

Abstract
Background: Hereditary cholestasis is a heterogeneous group of liver diseases that mostly show autosomal recessive inheritance. The phenotype of cholestasis is highly variable. Molecular genetic testing offers a useful approach to differentiate different types of cholestasis because some symptoms and findings overlap. Biallelic variants in USP53 have recently been reported in cholestasis phenotype. In this study we aim to characterize clinical findings and biological insights on a novel USP53 splice variant causing cholestasis phenotype and review of the literature. Methods and Results: We reported a novel splice variant (NM_019050.2:c.238-1G>C) in the USP53 gene via whole exome sequencing in a patient with cholestasis phenotype. This variant was confirmed by sanger sequencing and as a result of family segregation analysis; it was found to be a heterozygous state in the parents and the other healthy elder brother of our patient. According to in-silico analyses; the change in the splice region resulted in an increase in the length of exon 1, whereas the stop codon after the additional 3 amino acids (VTF) caused the protein to terminate prematurely. Thus, the mature USP53 protein, consisting of 1.073 amino acids, has been reduced to a small protein of 82 amino acids.Conclusions: We propose a model for the tertiary structure of USP53 for the first time, together with all these data, we support the association of biallelic variants of the USP53 gene with the cholestasis phenotype. We also presented a comparison of previously reported patients with the USP53-associated cholestasis phenotype to contribute to the literature.
MYO5B Novel Homozygous Variant c.2090+3A&gt;T Causes Intron Retention and is Related To Severe Cholestasis and Intractable Diarrhea
Authors: Zheng Yu , Peng Yuming , Zhang Shuju , Peng Yu , Zhao Hongmei , Chen Weijian , Yang Yongjia , Li Liping , Yin Qiang 
Published: August 2021

Abstract
Background

Myosin Vb (MYO5B) plays an important role in protein trafficking and recycling. Biallelic mutated MYO5B has been found related to microvillus inclusion disease (MVID) or predominant cholestatic liver disease or mixed the two diseases. This study aims to clarify the splicing alteration and associated clinical or pathological features of a newly identified homozygous variant, and thus to provide supporting information for disease mechanism and clinical treatment.
Results

Here we identified a novel homozygous variant c.2090 + 3A > T by exome sequencing in a female patient. Minigene assay using recombined vectors and cell transfection found 185bp intention of intron 17 in the mRNA, which was predicted a pre-termination of myoVb (p.Arg697fs*47) at the end of the head motor domain. Further bowel biopsies via immunohistochemistry and the electron microscope detected decreased microvillus and local lesion of microvillus inclusion in the mucosa of duodena. The patient presented intractable diarrhea alleviated from severe in early life to mild later. She also had congenital cholestasis, liver cirrhosis, cholelithiasis, hepatic cyst, corneal opacity, and failure to thrive. Medicines were supplied to alleviate symptoms, maintain her intestines and nutrition. Liver transplantation would be the final choice.
Conclusions

Our study reported a novel homozygous variant that altered splicing and further supported the underlying mechanism that led to complicated enterohepatic phenotypes. The results expanded clinical practice in understanding the genotype-phenotype correlation of MYO5B and associated disease management.
An Ethnobotanical and Ethnomedicinal Investigation of Phytomedical Knowledge and Practice of Medicinal Plants in Lingchuan County, Shanxi, China
Authors: Jin Shan , Zhang Shanshan , Shad Nasir , Naeem Abid , Yang Yandeng , Wu Shuaikai 
Published: August 2021

Abstract
BackgroundLingchuan County is the main producing area of Chinese medicinal materials in Shanxi Province, rich in medicinal plant resources. The aboriginal residents of Lingchuan County have certain traditional knowledge and experience in the use of medicinal plants and can effectively use medicinal plants to treat common diseases. MethodologyA field survey in 53 villages was carried out over one year, i.e., November 2017—August 2018. Ethnomedicinal data was collected through designed questionaries/interviews by involving 180 informants familiar with medicinal plants utilization. Documented data were evaluated using the quantitative indices. ResultsA total of 138 traditional medicinal plants were investigated, belonging to 123 genera of 60 families. Among them, Asteraceae was the dominant plant family with 19 species. Roots were the most commonly used plant parts, and the dominant life form was herbs. Quantitative analysis revealed that Forsythia suspensa was with higher (0.33) RFC value, and Scutellaria baicalensis was recorded with higher (0.91) UV. Treated diseases were categorized in 12 groups and evaluated by their FIC value, in which gynecological diseases having higher (0.93) FIC value followed by urinary system diseases. The most medicinal plants are used to clear away heat and relieve the surface. At the same time, people use boiled water to use medicinal plants. Most of the plants investigated are non-toxic. ConclusionThis study reported traditional herbal medicines for the first time to be used against various disorders in Lingchuan County, China and are still practiced by the local communities. Some of the new ethnobotanical claims documented in this investigation should need to be further explored clinically. The medicinal plants with the highest use values recorded in this study may signpost the probable existence of valuable phytochemical compounds that requires a search for prospective new drugs to cure many human disorders.
Family Support-Based Intervention Using A Mobile Application By Pharmacists For Older Adults With Diabetes: A Randomised Controlled Trial
Authors: Poonprapai Potjana , Lerkiatbundit Sanguan , Saengcharoen Woranuch - 
Published: August 2021

Abstract
Background Family support is crucial in the care of elderly patients with diabetes. Pharmacists have proven to play an important role in assisting patients to achieve goals of drug therapy. However, more information is needed to evaluate the potential benefits of family-based intervention through a mobile application by pharmacists in the elderly diabetes patients.Objective To evaluate the influences of family support-based intervention using a mobile application on glycaemic control and diabetes control-related outcomes in the older adults with diabetes. Setting A hospital in the south of Thailand.Method A randomised controlled trial was conducted with 9 months of follow-up. Family members in the intervention group (n = 78) received diabetes educational courses and encouragement via a mobile application from pharmacists to help their elderly relatives with diabetes in self-management tasks. The control group received usual care (n = 79). Main outcome measure Glycosylated haemoglobin (HbA1c).Results As compared to baseline, significant improvements were observed in the intervention patients for HbA1c, blood pressure, family behaviour in diabetes care, diabetes knowledge, self-management practices and medication adherence (P < 0.001). The intervention group showed greater decline in HbA1c levels relative to the control group (-0.97% vs. -0.12%; P = 0.001). Significant differences between groups for changes in blood pressure levels including scores of family behaviour, diabetes knowledge, self-management and medication adherence were found (P < 0.001), with the intervention group showing greater improvement. Conclusion Family support intervention via a mobile application by pharmacists is beneficial to diabetes care for the elderly. Trial registration number: TCTR20200615001 (date 13 June 2020, retrospectively registered).
Vitamin D Receptor Gene FokI Polymorphism Contributes to Increasing the Risk of Tuberculosis: Evidence from a Meta-Analysis
Authors: Li Bin , Li Yunxia , Wen Fei , Wang Zhaofen 
Published: August 2021

Abstract
Purpose In the present study, we explored the link between vitamin D receptor (VDR) FokI gene polymorphisms with tuberculosis(TB). Methods Based on a comprehensive search of PubMed, Embase, Web of Science, Elsevier Science Direct, Cochrane Library, CNKI, Wanfang, and Chongqing VIP databases, we searched case-control study on FokI gene polymor-phism and TB susceptivity. The Newcastle-Ottawa Scale(NOS) was used to evaluate the quality of the literature and extracted data, and R 4.0.5 software was used for the meta-analysis. Results: Among the 243 selected articles, 35 in the meta analysis. The meta-analysis showed that the FokI gene polymorphism allene gene model(f vs F, Odds ratio=1.22,95% confidence interval:1.11-1.36); dominant model (ff+fF vs F, Odds ratio=1.29,95% confidence interval:1.13-1.47); recessive model(ff vs fF+FF, Odds ratio=1.31,95% confidence interval:1.09-1.56); codominant (ff vs FF, Odds ra-tio=1.48,95% confidence interval:1.19-1.83); codominant(fF vs FF, Odds ratio=1.23,95% confidence interval:1.09-1.39). The meta-analysis indicates a high level of heterogeneity between the VDR FokI gene polymorphism and TB and the race is a source of heterogeneity in the results. Conclusion The present update meta-analysis suggest that FokI gene polymorphism is significantly associated with an increased risk of TB.