bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSMD8 Promotes Breast Cancer Progression and Attenuates Paclitaxel Sensitivity by Regulating the Expression of KIF10
Authors: Li Zhiyu , Li Chenyuan , Tu Yi , Yao Feng , Sun Shengrong , Liao Shichong , Wang Zhong 
Published: September 2021

Abstract
BackgroundBreast cancer (BC) patients were threatened by distant metastasis and drug resistance for a long time. The mechanism was to be explored urgently. Proteasome 26S subunit non-ATPase 8 (PSMD8) was associated with protein degradation, but little was known about its role in BC.MethodsThe bioinformatics analysis was obtained from public databases. Breast cancer cell lines were knocked down or overexpressed PSMD8/kinesin family member 10 (KIF10) and detected their ability of proliferation and invasion. epithelial-to-mesenchymal transition (EMT)-related markers were detected by the western blot and immunohistochemistry. In vivo models were established to verify the effect of PSMD8 on tumor growth and distant metastasis. Cells were exposed to paclitaxel (PTX) and examined their apoptosis by flow cytometry.ResultsIn the present study, we defined PSMD8 as a tumor-promoting molecule. PSMD8 was abnormally upregulated in BC tissues compared with adjacent tissues. High PSMD8 expression was related to poor prognosis in BC patients. The knockdown of PSMD8 attenuated the biological effect and epithelial-mesenchymal transition (EMT) progression in vitro and in vivo, and the overexpression of PSMD8 increased tumor progression in vitro. Furthermore, PSMD8 knockdown dramatically augmented the sensitivity of BC cells to paclitaxel, whereas PSMD8 overexpression showed the opposite effects. Mechanistic analysis indicated that PSMD8 activated the expression of kinesin family member 10 (KIF10) and its downstream extracellular regulated MAP kinase (ERK) signaling. Similar to PSMD8, we revealed that the expression of KIF10, which showed a positive correlation with PSMD8, was increased in BC tissues compared with adjacent tissues and associated with a poor prognosis of BC patients. The KIF10 knockdown significantly antagonized the facilitating effect of PSMD8 on the EMT program and chemotherapy tolerance. Finally, knocking down the level of KIF10 or inhibiting the ERK pathway in PSMD8-overexpressing cell lines partially blocked EMT progression and resistance against paclitaxel (PTX). ConclusionsIn conclusion, our results demonstrated that the PSMD8/KIF10 axis promoted BC development and PTX resistance via the ERK pathway. Our findings provide a theoretical basis for targeting PSMD8 to BC cells as a novel target for the treatment of BC.
Heavy Metals in Soils and Road Dusts in Akure City, Southwest Nigeria: Ecological and Health Risks
Authors: Adewumi Adeniyi JohnPaul 
Published: September 2021

Abstract
Expanded urbanization particularly in developing nations have as of late become a significant source of worry because of their adverse consequences on the environment. This study was conducted to determine the extent of pollution, sources, and potential ecological and health risks associated with heavy metals in Akure region, Nigeria's soils and street dusts. Thirty-four samples including 16 soils and street dusts samples each were gathered with two control samples gathered from a suburb a long way from the city. To collect fine particles, they were air dried and sieved. Chemical analysis of samples werecarried out utilizing Atomic Absorption Spectrometer (AAS) at Sustainable laboratory, a certified laboratory in Akure, Nigeria. Metals analysed were: As, Cd, Co, Cr, Cu, Ni, Pb, Zn and Fe. Contamination, ecological and health hazard evaluations were carried out utilizing universally acceptable indices. Potential sources were disentangle utilizing measurable technique like bivariate, factor and hierarchical cluster analyses. Results uncovered that the mean amount of Pb, Zn and Fe in street dusts were: 32.33 mg/kg, 72.53 mg/kg, and 115.21 mg/kg separately while in soil tests it was 34.34 mg/kg, 67.76 mg/kg, and 115.21 mg/kg. The examination further showed that metals particularly Cr present slight to high ecological dangers. Health hazard evaluation uncovered that the occupants of the area particularly kids are more inclined to non-cancer-causing health risks. Metals were mostly from anthropogenic sources. To reduce the impact of toxic metals in the city, it is proposed that ecological enactment and mindfulness be practiced.
Human Ovarian Cryopreservation: Vitrification Versus Slow Freezing From Histology To Gene Expression.
Authors: Jaeger Pauline , Fournier Cyrielle , Santamaria Claire , Fraison Eloise , Morel-Journel Nicolas , Benchaib Mehdi , Salle Bruno , Lornage Jacqueline , Labrune Elsa 
Published: September 2021

Abstract
Background:
Cryopreservation of ovarian tissue is one of the strategies offered to girls and women needing gonadotoxic treatment, as a means of preserving their fertility. There are two methods of ovarian tissue cryopreservation: slow freezing, the reference method, and vitrification, an alternative method. The aim of the present study was to evaluate which of the two is the best method for human ovarian tissue cryopreservation. Each ovary was divided into 3 groups: Fresh, Slow freezing and Vitrification. In each group a histological study to evaluate follicular density and quality; and an evaluation of 6 gene expression (CYP11A, STAR, GDF9, ZP3, CDK2, CDKN1A) were performed.
Results:
We observed no significant difference in follicular density within these 3 groups. Slow freezing altered the pool of primordial follicles compared to the Fresh tissue (31.8% vs 55.9%, p = 0.046, respectively). The expression of genes involved in steroidogenesis varied after cryopreservation compared to the fresh group; CYP11A was under-expressed in both freezing groups compared to the fresh group and significantly under-expressed in the slow freezing group (p = 0.01), STAR was over-expressed in the slow freezing group and significantly under-expressed in the vitrification group (p = 0.01). Regarding the expression of genes involved in cell cycle regulation, CDKN1A was significantly under-expressed in both freezing groups (slow freezing: p = 0.0008; vitrification: p = 0.03) compared to the fresh group.
Conclusion:
Vitrification had no effect on the histological quality of the follicles at any stage of development compared to Fresh tissue. There was no significant difference in gene expression between the two techniques.
Effects of Plant Growth Regulators On Transient Expression of Foreign Genes In Nicotiana Benthamiana L. Leaves
Authors: Li Ying , Sun Min , Wang Xin , Zhang Jing Yue , Da Wei Xiao , Jia Yun Ling , Pang Long Hai , Feng Qing Han 
Published: September 2021

Abstract
Background: In the last decades, autonomously replicating expression vectors based on plant geminivirus has been widely used as one of the most effective expression vectors in plant transient expression. Compared with those non-replicating expression vectors, autonomously replicating expression vectors based on plant geminivirus have many advantages. By using the bean yellow dwarf virus-derived expression vector and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins, and 6-benzyladenine, as three common plant growth regulators, on the efficiency of plant transient expression and biomass during the process of transient expression in Nicotiana benthamiana L. leaves. Results: With the increase of α-naphthalene acetic acid, gibberellins, and 6-benzyladenine (from 0.1 to 1.6 mg/L), the fresh weight, dry weight, and leaf area of the seedlings increased first and then returned to the levels similar to the controls (without plant growth regulators treatment). α-naphthalene acetic acid at 0.2 and 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.4 mg/L α-naphthalene acetic acid and was increased about 19%, compared to the controls. Gibberellins at 0.1 to 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.2 mg/L gibberellins and was increased about 25%. However, the 6-benzyladenine application led to decrease in the level of transient expression of green fluorescent protein. Conclusions: The moderate concentration of appropriate plant growth regulators could be beneficial to the expression of foreign proteins from the Agrobacterium-mediated transient expression system in plants. Thus, appropriate plant growth regulators could be considered as an exogenous component to be applied in recombinant protein production by plant-based transient expression systems.
Optimising Nutrients in the Culture Medium of Rhodosporidium Toruloides Enhances Lipids Production
Authors: Ye Zi , Sun Tongrui , Hao Huoye , He Yanling , Liu Xueyan , Guo Minrui , Chen Guogang 
Published: September 2021

Abstract
Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.
Analog/RF Performance Analysis of a-ITZO Thin Film Transistor
Authors: Jain Neeraj , Singh Kunal , Sharma Shashi Kant , Kumawat Renu 
Published: September 2021

Abstract
This work reports RF and analog performance analysis of an amorphous Indium Tin Zinc Oxide thin film transistor. The various parameters affecting the performance of a-ITZO TFT like drain current, drain conductance, output resistance, transconductance, transconductance generation factor, early voltage, intrinsic gain, capacitances, cut off frequency, maximum frequency of oscillation, transconductance frequency product, gain frequency product, gain bandwidth product and gain transconductance frequency product have been closely examined. The device is further analyzed to investigate the impact of variation in physical parameters viz. dielectric material, dielectric thickness (𝐷𝑡 ) and temperature (T) on the RF/Analog performance. Use of high-k dielectric material in the simulated structure has resulted in low subthreshold slope (SS) of 0.62 V/decade, On voltage (𝑉𝑜𝑛) of (- 0.29) V, 𝐼𝑜𝑛/𝐼𝑜𝑓𝑓 ratio of ~ 109 , intrinsic gain (𝐴𝑉) of 104.5 dB and gain frequency product (GFP) of 1.86 GHz. The best results for dielectric thickness variation are offered for dielectric thickness of 150 nm with SS of 0.22 V/decade, 𝑉𝑜𝑛 of (-0.26 V), 𝐼𝑜𝑛/𝐼𝑜𝑓𝑓 of ~ 1010 , (𝐴𝑉) of 175.69 dB and GFP of 2.39 GHz. For device reliability and stability study, temperature analysis has also been done. To demonstrate the circuit level implementation of the simulated structure, a resistive load inverter circuit is simulated and analyzed for different variations (high-k, 𝐷𝑡 and T). The results obtained are promising to meet the current display industry requirement. It has also been concluded that TFT with high-k material or thinner dielectric at T=300 K provides best performance.
Antineoplastic Effects of Erufosine on Small and Non-Small Lung Cancer Cells Through Induction of Apoptosis and Cell Cycle Arrest
Authors: Abdik Hüseyin 
Published: September 2021

Abstract
Background: Lung cancer (LC) is one of the most common types of cancer with a high mortality rate. Depending on molecular and histological properties, LC is divided into non–small-cell and small-cell lung cancer. Not only surgery but also radiotherapy, chemotherapy, or combination treatment are used for patients. However, the survival rate of LC is still very low. Erufosine (ErPC3) is a novel promising antineoplastic agent and inhibits the translocation of AKT to the plasma membrane by dephosphorylating AKT. Methods and Results: In the current study, the cell-type dependent effects of ErPC3 on cell viability, apoptotic situation, cell cycle distribution, related gene expression, and migration capacities of A549 and DMS 114 were investigated. As results, ErPC3 exhibited cytotoxic and pro-apoptotic properties against both cells, while DMS 114 was more affected. ErPC3 accumulated the cells in G2/M phase and blocked cell cycle. Proliferation markers were downregulated, while pro-apoptotic markers were upregulated in ErPC3 treated cells. Besides, ErPC3 displayed anti-migratory effect on A549 and DMS 114 compared to the control group according to scratch assay. Conclusion: These findings promise a treatment approach and drug development against LC. The obtained results from the recent study lead it necessary to carry out more detailed studies about ErPC3.
Modified Shoelace Dural Closure With Collagen Matrix in Extended Transsphenoidal Surgery
Authors: Nagata Yuichi , Takeuchi Kazuhito , Sasaki Hiroo , Mizuno Akihiro , Harada Hideyuki , Tanahashi Kuniaki , Araki Yoshio , Saito Ryuta 
Published: September 2021

Abstract
Extended endonasal transsphenoidal surgery (eTSS) offers a wide surgical field for various parasellar lesions; however, intraoperative high-flow cerebrospinal fluid (CSF) leakage is inevitable. Therefore, secure sellar reconstruction methods are essential to prevent postoperative CSF leakage. Although collagen matrix has been applied for dural reconstruction in neurosurgery, its suitability for application in extended eTSS remains unclear.Fifteen patients underwent modified shoelace dural closure using collagen matrix after lesionectomy via extended eTSS. In this technique, a collagen matrix, which was placed subdurally (inlay graft), was continuously sutured with both open dural edges like a shoelace. Then, another collagen matrix was placed epidurally (onlay graft), and rigid reconstruction was performed using the septal bone and a resorbable fixation mesh. Postoperative CSF leakage did not occur in fourteen patients, but did in one patient with tuberculum sellae meningioma. In this case, the CSF leakage point was detected just around the area between the coagulated dura and the adjacent collagen matrix. The collagen matrix harvested from this area was pathologically examined; neovascularization and fibroblastic infiltration into the collagen matrix were not detected. On the other hand, neovascularization and fibroblast infiltration into the collagen matrix were apparent on the surface of the collagen matrix harvested from the non-CSF leakage area.Our novel dural closure technique using collagen matrix could be an effective option for sellar reconstruction in extended eTSS; however, it should be applied in patients in whom normal dural edges are preserved.Clinical trial registrationNot applicable
Tagraxofusp Refractory Blastic Plasmacytoid Dendritic Cell Neoplasm With Loss of CD123 Expression: Case and Review of Literature
Authors: Gulati Rohit , Abu-Salah Asma , Salous Tareq , Nassiri Mehdi 
Published: September 2021

Abstract
Background: Tagraxofusp, a CD123 based targeted immunotherapy, was recently approved to treat blastic plasmacytoid dendritic cell neoplasm (BPDCN) with excellent response. Also, a subset of BPDCN showed resistance to tagraxofusp. These resistant cases continue to express CD123, which forms the basis of the continued utility of tagraxofusp in newer combination chemotherapies to overcome resistance in BPDCN. Case presentation: Herein we report a case of an elderly male with BPDCN that initially achieved complete remission on primary treatment with tagraxofusp. However, BPDCN relapsed after 1.5 years while on treatment with loss of CD123 expression. Conclusions: This case highlights a potential limitation of current and upcoming tagraxofusp based therapies, at least in a subset of refractory BPDCN. We believe our report will serve as a sentinel to incite future investigations involving alternate resistance mechanisms in BDPCN.
Predicting Surgical Factors for Unplanned Overnight Admission in Ambulatory Arthroscopic Surgery of the Knee: A Prospective Cohort in 184 patients
Authors: Watcharopas Ratthapoom , Saengpetch Nadhaporn , Kijkunasathian Chusak , Limitlaohaphan Chalermchai , Lertbutsayanukul Chatchawan , Vijittrakarnrung Chaiyanun , Arnuntasupakul Vanlapa , Sangkum Lisa , Sa-ngasoongsong Paphon 
Published: September 2021

Abstract
Background: Unplanned overnight admission (UOA) is an important indicator for quality of care with ambulatory knee arthroscopic surgery (AKAS). However, few studies have explored the factors related to the UOA and how to predict UOA after AKAS. This study aimed to evaluate the effectiveness of a standardized perioperative protocol for the AKAS with UOA and identify whether a correlation exists between the perioperative surgical factors and UOA in the patients undergoing AKAS. Methods: A prospective cohort study was conducted, between October 2017 and March 2021, in 184 patients. All patients operated on standard AKAS protocol. The UOA was defined as overnight hospitalization of a patient undergoing AKAS. Demographic and perioperative data were recorded, and the procedure was categorized based on the surgical invasiveness based on less invasive (n = 65) and more complex surgery (n = 119). The clinical risk factors for UOA were identified and analyzed with multivariate logistic regression analysis. Results: The incidence of UOA in the more complex group (17 cases, 14.3%) was significantly higher than in the less invasive group (3 cases, 4.6%) (p = 0.049), with the incidence of readmission as 0%. The perioperative factors significantly associated with UOA were age, more complex surgery, and tourniquet time (p < 0.10 all). However, the multivariate regression analysis revealed that tourniquet time was the only significant predictor for UOA (odds ratio = 1.045, 95% confidence interval = 1.022 to 1.067, p = 0.0001). The optimal cut-off points of tourniquet time for predicting UOA with the highest Youden index in the less invasive and more complex groups were 56 minutes and 107 minutes, respectively. Conclusion: The UOA after AKAS is more common in more complex surgery compared to less invasive surgery. Many factors—such as patient factors, surgical invasiveness, and tourniquet time—were also significantly associated with the unplanned admission. However, the results from this study showed that, under strict perioperative management protocol, tourniquet time is the only independent predictor for UOA.
Effect of SARs-CoV-2 Pandemic on Infection Prevention Behaviors and Bacterial Burden of High Touch Surfaces in a Medical/surgical Setting
Authors: Resendiz Marisol , DNP Dawn M. Blanchard , Lustik Michael B. , Horseman Timothy S. , West Gordon F. 
Published: September 2021

Abstract
Background: This study aimed to determine the longitudinal efficacy of ultraviolet germicidal disinfection (UV-C) in a non-terminal disinfection context. Moreover, factors influencing enhanced infection prevention behaviors during the SARS-CoV-2 pandemic were evaluated. Methods: Sixty nursing staff from three medical/surgical wards in a large military hospital were recruited for a survey and microbiological sampling of high-touch surfaces (stethoscope, personal electronic device, common access card, and hospital ID badge) and portable medical equipment (wheelchairs and mobile commodes). Surveys included hand hygiene estimates, frequency/method of cleaning, perception of UV-C, and factors influencing the use of enhanced disinfection tools. Surveys and microbiological samples were performed prior to and after the installation of a rapid, automated ultraviolet disinfection enclosure for staff use. Both time points preceded the SARS-CoV-2 pandemic in the United States. A final survey/sampling time point was carried out eight months after the declaration of the COVID-19 pandemic. Results: Participants’ hand hygiene frequency did not increase throughout the study, with >80% reporting a minimum of 4 hand hygiene events per patient hour. The cleaning frequency of high-touch surfaces (but not portable medical equipment) increased after installation of a UV-C disinfection tool and was sustained eight months into the COVID-19 pandemic. While a modest decrease in bacterial burden was observed after UV-C intervention, a more significant reduction was observed across all surfaces during pandemic time sampling , though no detectable decrease in pathogenic contamination was observed at either time point. Motivators of UV-C use included fear of SARS-CoV-2 contamination and transmission, ease of device use, and access to rapid, automated disinfection tools while deterrents reported included technical concerns, lack of time, and preference for other disinfection methods. Conclusions: Automated, rapid-cycle UV-C disinfection can be efficacious for high-touch surfaces not currently governed by infection prevention and control guidelines. The introduction of enhanced disinfection tools like UV-C can enhance the overall cleaning frequency and is correlated with mild decreases in bacterial burden of high-touch surfaces, this is enhanced during periods of heightened infection threat. Evidence from this study offers insights into the factors which prompt healthcare workers to internalize/dismiss enhanced infection prevention procedures.
Predictors of Radiation-induced Hypothyroidism in Nasopharyngeal Carcinoma Survivors after Intensity-modulated Radiotherapy
Authors: Zhai Ruiping , Lyu Yingchen , Ni Mengshan , Kong Fangfang , Du Chengrun , Hu Chaosu , ying hongmei 
Published: September 2021

Abstract
BackgroundThe aim of the study is to identify clinical and dosimetric factors that could predict the risk of hypothyroidism in nasopharyngeal carcinoma (NPC) patients following intensity-modulated radiotherapy (IMRT). MethodsA total of 404 non-metastatic NPC patients were included in our study. All patients were treated with IMRT. The thyroid function were performed for all patients before and after radiation at regular intervals. The time onset for developing hypothyroidism was defined as the time interval between the completion of RT and the first recorded abnormal thyroid hormone test. The cumulative incidence rates of hypothyroidism were estimated using Kaplan-Meier method. Univariate and multivariate Cox regression analyses were performed to detect the most promising factors that were associated with hypothyroidism. ResultsMedian follow up was 60.6 months. The 3-, 5- and 7- year cumulative incidence rate of hypothyroidism was 39.4%, 49.1% and 54.7%, respectively. The median time to primary hypothyroidism and central hypothyroidism were 15.4 months (range: 2.9-83.8 months) and 29.9 months (range: 19.8-93.6 months), respectively. Univariate and multivariate analyses revealed that younger age, female gender and small thyroid volume were the most important factors in predicting the risk of hypothyroidism. Dtmean (mean dose of thyroid), V30-V50 (percentage of thyroid volume receiving a certain dose level) and VS45-VS60 (the absolute volumes of thyroid spared from various dose levels) remained statistically significant in multivariate analyses. Cutoff points of 45 Gy (Dtmean), 80% (Vt40) and 5 cm3 (VS45Gy) were identified to classify patients as high-risk or low-risk group. ConclusionThyroid Vt40 predicted the risk of hypothyroidism after IMRT for NPC patients. We recommended plan optimization objectives to reduce thyroid V40 to 80%.Trial registration: retrospectively registered.