bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 molecular testing in Greek hospital paediatric departments: a nationwide study
Authors: Michos Athanasios , Savvidou Parthena , Syridou Garyfallia , Eleftheriou Eirini , Iosifidis Elias , Grivea Ioanna , Spoulou Vana , Galanakis Emmanouil , Syrogiannopoulos George , Tsolia Maria , Roilides Emmanuel , Papaevangelou Vassiliki 
Published: February 2021

Abstract
As most children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present with mild symptoms or they are asymptomatic, the optimal strategy for molecular testing it is not well defined. The aim of the study was to determine the extent and aetiology of molecular testing for SARS-CoV-2 in Greek paediatric departments during the first phase of the pandemic and identify possible differences in incidence, depending on the age group and geographical area. We conducted a nationwide study of molecular testing for SARS-CoV-2 of children in paediatric departments between March and June 2020. A total of 65 paediatric departments participated in the study, representing 4901 children who were tested for SARS-CoV-2 and 90 (1.8%) were positive. Most paediatric cases were associated with topical outbreaks. Adolescents 11–16 years had the highest positivity rate (3.6%) followed by children 6–10 years (1.9%). However, since the testing rate significantly differed between age groups, the modified incidence of SARS-CoV-2 infection per age group was highest in infants <1 year (19.25/105 population). Most children tested presented with fever (70.9%), respiratory (50.1%) or gastrointestinal symptoms (28.1%). Significant differences were detected between public and private hospitals regarding the positivity rate (2.34% vs. 0.39%, P-value <0.001). Significant variation in SARS-CoV-2 molecular testing positivity rate and incidence between age groups indicate discrepancies in risk factors among different age groups that shall be considered when ordering molecular testing.
Health Care Organizations: Soft Target during COVID-19 Pandemic
Authors: De Cauwer Harald G. , Somville Francis 
Published: February 2021

AbstractHealth care organizations have been challenged by the coronavirus disease 2019 (COVID-19) pandemic for some time, while in January 2020, it was not immediately suspected that it would take such a global expansion. In the past, other studies have already pointed out that health care systems, and more specifically hospitals, can be a so-called “soft target” for terrorist attacks. This report has now examined whether this is also the case in the context of the COVID-19 pandemic.During the lockdown, hospitals turned out to be the only remaining soft targets for attacks, given that the other classic targets were closed during the lockdown. On the other hand, other important factors have limited the risk of such attacks in hospitals. The main delaying and relative risk-reducing factors were the access control on temperature and wearing a mask, no visits allowed, limited consultations, and investigations.But even then, health care systems and hospitals were prone to (cyber)terrorism, as shown by other COVID-19-related institutions, such as pharmaceuticals involved in developing vaccines and health care facilities involved in swab testing and contact tracing. Counter-terrorism medicine (CTM) and social behavioral science can reduce the likelihood and impact of terrorism, but cannot prevent (state-driven) cyberterrorism and actions of lone wolves and extremist factions.
Pre-trial detention and legal defence in Latin America
Authors: Fondevila Gustavo , Quintana-Navarrete Miguel 
Published: February 2021

AbstractPre-trial detention is used extensively in Latin America as a systematic practice implemented by courts. Despite this fact, few empirical studies have analysed the role of defence attorneys in pre-trial incarceration. This paper attempts to describe the actions taken by lawyers in order to free their clients during the judicial process, using new empirical evidence collected from the incarcerated population in Argentina, Brazil, El Salvador, Chile, Mexico and Peru. Results suggest that public defenders request conditional release less frequently but do so more effectively than their private counterparts.
Weak Crust in Southeast Tibetan Plateau Revealed by Lg‐Wave Attenuation Tomography: Implications for Crustal Material Escape
Authors: He Xi , Zhao Lian‐Feng , Xie Xiao‐Bi , Tian Xiaobo , Yao Zhen‐Xing 
Published: February 2021

AbstractThe continuous convergence between the Indian and Eurasian plates caused massive lithospheric deformation in the Tibetan Plateau and led to excessive crustal material escaping through its southeastern margin. However, the mechanisms that accommodate the escaping materials and whether they intrude into the Indochina Peninsula are continually under debate. Seismic Lg waves mostly propagate within the crust waveguide with an amplitude decay that is sensitive to crustal material properties, such as temperature, partial melting, and fracture. Therefore, Lg attenuation can be a useful indicator of potential crustal material escape. In this study, we developed a high‐resolution broadband Lg attenuation model in this region between 0.05 and 10.0 Hz, with the resolution reaching 1° in regions with dense raypath coverage. Prominent low‐Q anomalies beneath the southeastern margin of the Tibetan Plateau, correlating with previously observed low velocity, high conductivity and high Poisson’s ratio, may indicate possible high temperature and/or partial melting within a relatively weak crust, and suggest a north‐south interconnected corridor for gravity‐driven material flow. Through our results and other geological and geophysical observations, a dynamic model is suggested here by combining shallow rigid block extrusion and deep viscous crustal flow to explain crustal material escape in the southeastern margin of the Tibetan Plateau. Additionally, neither shallow extrusion nor deep material flow enter the Indochina Peninsula based on the relatively high Q values, which indicate a stronger crust there.
Needle Propagation and Twinkling Characteristics
Authors: Hare B. M. , Scholten O. , Dwyer J. , Strepka C. , Buitink S. , Corstanje A. , Falcke H. , Hörandel J. R. , Huege T. , Krampah G. K. , Mitra P. , Mulrey K. , Nelles A. , Pandya H. , Rachen J. P. , Thoudam S. , Trinh T. N. G. , ter Veen S. , Winchen T. 
Published: February 2021

AbstractRecently, a new lightning phenomena, termed needles, has been observed in both VHF and in optical along positive lightning leaders. They appear as small (<100 m) leader branches that undergo dielectric breakdown at regular intervals (called twinkles). Providing a coherent and consistent explanation for this phenomenon is challenging as each twinkle is a form of negative breakdown that propagates away from the positive leader. In this study, we provide detailed observations of needles in VHF, observed during two lightning flashes. We show distributions of different needle properties, including twinkle propagation speeds, time between twinkles, and needle lengths, among others. We show a return stroke and multiple recoil leaders that quench needle activity. We also show that nearby needle activity does not seem to correlate together, and that needle twinkling can slow down by 10%–30% per twinkle. We conclude by presenting possibilities for how the positive leader could induce negative propagation away from the positive channel, and we argue that twinkles can propagate like a stepped leader or like a recoil leader depending on the temperature of the needle, which implies that needle twinkles can probably propagate without emitting VHF.
Alternative splicing and cancer: a systematic review
Authors: Zhang Yuanjiao , Qian Jinjun , Gu Chunyan , Yang Ye 
Published: February 2021

AbstractThe abnormal regulation of alternative splicing is usually accompanied by the occurrence and development of tumors, which would produce multiple different isoforms and diversify protein expression. The aim of the present study was to conduct a systematic review in order to describe the regulatory mechanisms of alternative splicing, as well as its functions in tumor cells, from proliferation and apoptosis to invasion and metastasis, and from angiogenesis to metabolism. The abnormal splicing events contributed to tumor progression as oncogenic drivers and/or bystander factors. The alterations in splicing factors detected in tumors and other mis-splicing events (i.e., long non-coding and circular RNAs) in tumorigenesis were also included. The findings of recent therapeutic approaches targeting splicing catalysis and splicing regulatory proteins to modulate pathogenically spliced events (including tumor-specific neo-antigens for cancer immunotherapy) were introduced. The emerging RNA-based strategies for the treatment of cancer with abnormally alternative splicing isoforms were also discussed. However, further studies are still required to address the association between alternative splicing and cancer in more detail.
Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents
Authors: Yao Wei , Lin Song , Su Jin , Cao Qianqian , Chen Yueyue , Chen Jiaxu , Zhang Zhentao , Hashimoto Kenji , Qi Qi , Zhang Ji-chun 
Published: February 2021

AbstractThe transcription factor erythroid 2-related factor 2 (Nrf2) and brain-derived neurotrophic factor (BDNF) play a key role in depression. However, the molecular mechanisms underlying the crosstalk between Nrf2 and BDNF in depression remain unclear. We examined whether Nrf2 regulates the transcription of Bdnf by binding to its exon I promoter. Furthermore, the role of Nrf2 and BDNF in the brain regions from mice with depression-like phenotypes was examined. Nrf2 regulated the transcription of Bdnf by binding to its exon I promoter. Activation of Nrf2 by sulforaphane (SFN) showed fast-acting antidepressant-like effects in mice by activating BDNF as well as by inhibiting the expression of its transcriptional repressors (HDAC2, mSin3A, and MeCP2) and revising abnormal synaptic transmission. In contrast, SFN did not affect the protein expression of BDNF and its transcriptional repressor proteins in the medial prefrontal cortex (mPFC) and hippocampus, nor did it reduce depression-like behaviors and abnormal synaptic transmission in Nrf2 knockout mice. In the mouse model of chronic social defeat stress (CSDS), protein levels of Nrf2 and BDNF in the mPFC and hippocampus were lower than those of control and CSDS-resilient mice. In contrast, the protein levels of BDNF transcriptional repressors in the CSDS-susceptible mice were higher than those of control and CSDS-resilient mice. These data suggest that Nrf2 activation increases the expression of Bdnf and decreases the expression of its transcriptional repressors, which result in fast-acting antidepressant-like actions. Furthermore, abnormalities in crosstalk between Nrf2 and BDNF may contribute to the resilience versus susceptibility of mice against CSDS.
The colour stability of crystallized acetyl resin material in comparison to other restorative materials. An in-vitro study
Authors: Aburaisi Sarah , Basha Abdulkarim , Al Najjar Khalid , Al Saqat Husam , Al Askar Faisal , Al Nazer Fareed 
Published: February 2021

Abstract
Aim
To investigate the colour stability of Crystalized Acetyl Resin, in comparison to three other aesthetic restorative materials, when immersed in different staining aqueous solutions.

Materials and methods
Fifteen disc-shaped specimens each, of the four materials tested, were immersed in either DW or 4 staining solutions. CIE L*a*b* values were recorded using a digital spectrophotometer on a weekly basis for 4 weeks. Data were compared using three- way/two-way ANOVA along with auto-regressive model.

Results
Two-way Anova indicates that there is a significant colour change in all materials over time when immersed in the different staining solutions. Auto-regressive model showed that the colour change in each successive week was statistically significant (P < 0.001).

Conclusion
The most staining solution affecting the materials during this study was black coffee. Within the limitation of this study, Acetyl Resin was the most colour stable material among the other materials.
Chemically induced local lattice distortions versus structural phase transformations in compositionally complex alloys
Authors: Ikeda Yuji , Gubaev Konstantin , Neugebauer Jörg , Grabowski Blazej , Körmann Fritz 
Published: February 2021

AbstractRecent experiments show that the chemical composition of body-centered cubic (bcc) refractory high entropy alloys (HEAs) can be tuned to enable transformation-induced plasticity (TRIP), which significantly improves the ductility of these alloys. This calls for an accurate and efficient method to map the structural stability as a function of composition. A key challenge for atomistic simulations is to separate the structural transformation between the bcc and the ω phases from the intrinsic local lattice distortions in such chemically disordered alloys. To solve this issue, we develop a method that utilizes a symmetry analysis to detect differences in the crystal structures. Utilizing this method in combination with ab initio calculations, we demonstrate that local lattice distortions largely affect the phase stability of Ti–Zr–Hf–Ta and Ti–Zr–Nb–Hf–Ta HEAs. If relaxation effects are properly taken into account, the predicted compositions near the bcc–hcp energetic equilibrium are close to the experimental compositions, for which good strength and ductility due to the TRIP effect are observed.
Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids
Authors: Hong Lewis Z. , Zhou Lihan , Zou Ruiyang , Khoo Chin Meng , Chew Adeline Lai San , Chin Chih-Liang , Shih Shian-Jiun 
Published: February 2021

AbstractAberrant miRNA expression has been associated with many diseases, and extracellular miRNAs that circulate in the bloodstream are remarkably stable. Recently, there has been growing interest in identifying cell-free circulating miRNAs that can serve as non-invasive biomarkers for early detection of disease or selection of treatment options. However, quantifying miRNA levels in biofluids is technically challenging due to their low abundance. Using reference samples, we performed a cross-platform evaluation in which miRNA profiling was performed on four different qPCR platforms (MiRXES, Qiagen, Applied Biosystems, Exiqon), nCounter technology (NanoString), and miRNA-Seq. Overall, our results suggest that using miRNA-Seq for discovery and targeted qPCR for validation is a rational strategy for miRNA biomarker development in clinical samples that involve limited amounts of biofluids.
Optogenetic current in myofibroblasts acutely alters electrophysiology and conduction of co-cultured cardiomyocytes
Authors: Kostecki Geran M. , Shi Yu , Chen Christopher S. , Reich Daniel H. , Entcheva Emilia , Tung Leslie 
Published: February 2021

AbstractInteractions between cardiac myofibroblasts and myocytes may slow conduction and generate spontaneous beating in fibrosis, increasing the chance of life-threatening arrhythmia. While co-culture studies have shown that myofibroblasts can affect cardiomyocyte electrophysiology in vitro, the extent of myofibroblast-myocyte electrical conductance in a syncytium is unknown. In this neonatal rat study, cardiac myofibroblasts were transduced with Channelrhodopsin-2, which allowed acute and selective increase of myofibroblast current, and plated on top of cardiomyocytes. Optical mapping revealed significantly decreased conduction velocity (− 27 ± 6%, p < 10–3), upstroke rate (− 13 ± 4%, p = 0.002), and action potential duration (− 14 ± 7%, p = 0.004) in co-cultures when 0.017 mW/mm2 light was applied, as well as focal spontaneous beating in 6/7 samples and a decreased cycle length (− 36 ± 18%, p = 0.002) at 0.057 mW/mm2 light. In silico modeling of the experiments reproduced the experimental findings and suggested the light levels used in experiments produced excess current similar in magnitude to endogenous myofibroblast current. Fitting the model to experimental data predicted a tissue-level electrical conductance across the 3-D interface between myofibroblasts and cardiomyocytes of ~ 5 nS/cardiomyocyte, and showed how increased myofibroblast-myocyte conductance, increased myofibroblast/myocyte capacitance ratio, and increased myofibroblast current, which occur in fibrosis, can work in tandem to produce pro-arrhythmic increases in conduction and spontaneous beating.
Effects of maize (Zea mays) genotypes and microbial sources in shaping fall armyworm (Spodoptera frugiperda) gut bacterial communities
Authors: Mason Charles J. , Hoover Kelli , Felton Gary W. 
Published: February 2021

AbstractPlants can have fundamental roles in shaping bacterial communities associated with insect herbivores. For larval lepidopterans (caterpillars), diet has been shown to be a driving force shaping gut microbial communities, where the gut microbiome of insects feeding on different plant species and genotypes can vary in composition and diversity. In this study, we aimed to better understand the roles of plant genotypes, sources of microbiota, and the host gut environment in structuring bacterial communities. We used multiple maize genotypes and fall armyworm (Spodoptera frugiperda) larvae as models to parse these drivers. We performed a series of experiments using axenic larvae that received a mixed microbial community prepared from frass from larvae that consumed field-grown maize. The new larval recipients were then provided different maize genotypes that were gamma-irradiated to minimize bacteria coming from the plant during feeding. For field-collected maize, there were no differences in community structure, but we did observe differences in gut community membership. In the controlled experiment, the microbial inoculation source, plant genotype, and their interactions impacted the membership and structure of gut bacterial communities. Compared to axenic larvae, fall armyworm larvae that received frass inoculum experienced reduced growth. Our results document the role of microbial sources and plant genotypes in contributing to variation in gut bacterial communities in herbivorous larvae. While more research is needed to shed light on the mechanisms driving this variation, these results provide a method for incorporating greater gut bacterial community complexity into laboratory-reared larvae.
Outcomes of a 2-year treat-and-extend regimen with aflibercept for diabetic macular edema
Authors: Hirano Takao , Toriyama Yuichi , Takamura Yoshihiro , Sugimoto Masahiko , Nagaoka Taiji , Sugiura Yoshimi , Okamoto Fumiki , Saito Michiyuki , Noda Kousuke , Yoshida Shigeo , Ishibazawa Akihiro , Sawada Osamu , Murata Toshinori 
Published: February 2021

AbstractThis prospective, open-label, single-arm, non-randomized clinical trial, assessed the efficacy of a 2-year treat-and-extend (T&E) regimen involving intravitreal aflibercept injection (IAI), with the longest treatment interval set to 16 weeks, and adjunct focal/grid laser in diabetic macula edema (DME) patients. We examined 40 eyes (40 adults) with fovea-involving DME from 8 Japanese centers between April 2015 and February 2017. Participants received IAI with an induction period featuring monthly injections and a subsequent T&E period featuring 8–16-week injection interval, adjusted based on optical coherence tomography findings. The primary endpoints were mean changes in the best-corrected visual acuity (BCVA) and central subfield macular thickness (CST) from baseline. Thirty patients (75%) completed the 2-year follow-up. The mean BCVA and CST changed from 60.5 ± 15.6 letters and 499.2 ± 105.6 µm at baseline to 66.6 ± 17.1 letters (P = 0.217) and 315.2 ± 79.0 µm (P < 0.001), respectively, after 2 years. The treatment interval was extended to 12 and 16 weeks in 6.7% and 66.7% of patients, respectively, at the end of 2 years. The T&E aflibercept regimen with the longest treatment interval set to 16 weeks, with adjunct focal/grid laser may be a rational 2-year treatment strategy for DME.
MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
Authors: Duan Wenrui , Tang Shirley , Gao Li , Dotts Kathleen , Fink Andrew , Kalvala Arjun , Aguila Brittany , Wang Qi-En , Villalona-Calero Miguel A. 
Published: February 2021

AbstractThe Fanconi Anemia (FA) pathway is essential for human cells to maintain genomic integrity following DNA damage. This pathway is involved in repairing damaged DNA through homologous recombination. Cancers with a defective FA pathway are expected to be more sensitive to cross-link based therapy or PARP inhibitors. To evaluate downstream effectors of the FA pathway, we studied the expression of 734 different micro RNAs (miRNA) using NanoString nCounter miRNA array in two FA defective lung cancer cells and matched control cells, along with two lung tumors and matched non-tumor tissue samples that were deficient in the FA pathway. Selected miRNA expression was validated with real-time PCR analysis. Among 734 different miRNAs, a cluster of microRNAs were found to be up-regulated including an important cancer related micro RNA, miR-200C. MiRNA-200C has been reported as a negative regulator of epithelial-mesenchymal transition (EMT) and inhibits cell migration and invasion by promoting the upregulation of E-cadherin through targeting ZEB1 and ZEB2 transcription factors. miRNA-200C was increased in the FA defective lung cancers as compared to controls. AmpliSeq analysis showed significant reduction in ZEB1 and ZEB2 mRNA expression. Our findings indicate the miRNA-200C potentially play a very important role in FA pathway downstream regulation.
Erythrocytes 3D genome organization in vertebrates
Authors: Ryzhkova Anastasia , Taskina Alena , Khabarova Anna , Fishman Veniamin , Battulin Nariman 
Published: February 2021

AbstractGeneration of mature red blood cells, consisting mainly of hemoglobin, is a remarkable example of coordinated action of various signaling networks. Chromatin condensation is an essential step for terminal erythroid differentiation and subsequent nuclear expulsion in mammals. Here, we profiled 3D genome organization in the blood cells from ten species belonging to different vertebrate classes. Our analysis of contact maps revealed a striking absence of such 3D interaction patterns as loops or TADs in blood cells of all analyzed representatives. We also detect large-scale chromatin rearrangements in blood cells from mammals, birds, reptiles and amphibians: their contact maps display strong second diagonal pattern, representing an increased frequency of long-range contacts, unrelated to TADs or compartments. This pattern is completely atypical for interphase chromosome structure. We confirm that these principles of genome organization are conservative in vertebrate erythroid cells.