bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep learning-based gene selection in comprehensive gene analysis in pancreatic cancer
Authors: Mori Yasukuni , Yokota Hajime , Hoshino Isamu , Iwatate Yosuke , Wakamatsu Kohei , Uno Takashi , Suyari Hiroki 
Published: August 2021

AbstractThe selection of genes that are important for obtaining gene expression data is challenging. Here, we developed a deep learning-based feature selection method suitable for gene selection. Our novel deep learning model includes an additional feature-selection layer. After model training, the units in this layer with high weights correspond to the genes that worked effectively in the processing of the networks. Cancer tissue samples and adjacent normal pancreatic tissue samples were collected from 13 patients with pancreatic ductal adenocarcinoma during surgery and subsequently frozen. After processing, gene expression data were extracted from the specimens using RNA sequencing. Task 1 for the model training was to discriminate between cancerous and normal pancreatic tissue in six patients. Task 2 was to discriminate between patients with pancreatic cancer (n = 13) who survived for more than one year after surgery. The most frequently selected genes wereACACB,ADAMTS6,NCAM1, andCADPSin Task 1, andCD1D,PLA2G16,DACH1, andSOWAHAin Task 2. According to The Cancer Genome Atlas dataset, these genes are all prognostic factors for pancreatic cancer. Thus, the feasibility of using our deep learning-based method for the selection of genes associated with pancreatic cancer development and prognosis was confirmed.
Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
Authors: Oliveira Hellen Braga Martins , Selis Nathan das Neves , Sampaio Beatriz Almeida , Júnior Manoel Neres Santos , de Carvalho Suzi Pacheco , de Almeida Jéssica Bomfim , Almeida Palloma Porto , da Silva Icaro Bonyek Santos , Oliveira Caline Novais Teixeira , Brito Thamara Louisy Santos , da Silva Letícia de Oliveira , Teixeira Mariana Morais , Coelho Hanna Izadora Laís Novaes , Barbosa Camila Dutra , Andrade Yasmin Monara Ferreira de Sousa , Bittencourt Rafaela de Souza , Viana Jully Chayra Santos , Campos Guilherme Barreto , Timenetsky Jorge , Uetanabaro Ana Paula T. , Yatsuda Regiane , Marques Lucas Miranda 
Published: August 2021

AbstractMethicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
Effect of adding hydrochlorothiazide to usual treatment of patients with acute decompensated heart failure: a randomized clinical trial
Authors: Piardi Diogo Silva , Butzke Maurício , Mazzuca Ana Carolina Martins , Gomes Bruna Sessim , Alves Sofia Giusti , Kotzian Bruno Jaskulski , Ghisleni Eduarda Chiesa , Giaretta Vanessa , Bellaver Priscila , Varaschin Gabrielle Aguiar , Garbin Arthur Pereira , Beck-da-Silva Luís 
Published: August 2021

AbstractAcute decompensated heart failure (ADHF) is the leading cause of hospitalization in patients aged 65 years or older, and most of them present with congestion. The use of hydrochlorothiazide (HCTZ) may increase the response to loop diuretics. To evaluate the effect of adding HCTZ to furosemide on congestion and symptoms in patients with ADHF. This randomized clinical trial compared HCTZ 50 mg versus placebo for 3 days in patients with ADHF and signs of congestion. The primary outcome of the study was daily weight reduction. Secondary outcomes were change in creatinine, need for vasoactive drugs, change in natriuretic peptides, congestion score, dyspnea, thirst, and length of stay. Fifty-one patients were randomized—26 to the HCTZ group and 25 to the placebo group. There was an increment of 0.73 kg/day towards additional weight reduction in the HCTZ group (HCTZ: − 1.78 ± 1.08 kg/day vs placebo: − 1.05 ± 1.51 kg/day; p = 0.062). In post hoc analysis, the HCTZ group demonstrated significant weight reduction for every 40 mg of intravenous furosemide (HCTZ: − 0.74 ± 0.47 kg/40 mg vs placebo: − 0.33 ± 0.80 kg/40 mg; p = 0.032). There was a trend to increase in creatinine in the HCTZ group (HCTZ: 0.50 ± 0.37 vs placebo: 0.27 ± 0.40; p = 0.05) but no significant difference in onset of acute renal failure (HCTZ: 58% vs placebo: 41%; p = 0.38). No differences were found in the remaining outcomes. Adding hydrochlorothiazide to usual treatment of patients with acute decompensated heart failure did not cause significant difference in daily body weight reduction compared to placebo. In analysis adjusted to the dose of intravenous furosemide, adding HCTZ 50 mg to furosemide resulted in a significant synergistic effect on weight loss.Trial registration: The Brazilian Clinical Trials Registry (ReBEC), a publically accessible primary register that participates in the World Health Organization International Clinical Trial Registry Platform; number RBR-5qkn8h. Registered in 23/07/2019 (retrospectively), http://www.ensaiosclinicos.gov.br/rg/RBR-5qkn8h/.
Using a material database and data fusion method to accelerate the process model development of high shear wet granulation
Authors: Wang Zheng , Cao Junjie , Li Wanting , Wang Yawen , Luo Gan , Qiao Yanjiang , Zhang Yanling , Xu Bing 
Published: August 2021

AbstractHigh shear wet granulation (HSWG) has been wildly used in manufacturing of oral solid dosage (OSD) forms, and process modeling is vital to understanding and controlling this complex process. In this paper, data fusion and multivariate modeling technique were applied to develop a formulation-process-quality model for HSWG process. The HSWG experimental data from both literature and the authors’ laboratory were fused into a single and formatted representation. A material database and material matching method were used to compensate the incomplete physical characterization of literature formulation materials, and dimensionless parameters were utilized to reconstruct process variables at different granulator scales. The exploratory study on input materials properties by principal component analysis (PCA) revealed that the formulation data collected from different articles generated a formulation library which was full of diversity. In prediction of the median granule size, the partial least squares (PLS) regression models derived from literature data only and a combination of literature data and laboratory data were compared. The results demonstrated that incorporating a small number of laboratory data into the multivariate calibration model could help significantly reduce the prediction error, especially at low level of liquid to solid ratio. The proposed data fusion methodology was beneficial to scientific development of HSWG formulation and process, with potential advantages of saving both experimental time and cost.
Thermosuperrepellency of a hot substrate caused by vapour percolation
Authors: Schmidt J. Benedikt , Hofmann Julian , Tenzer Fabian M. , Breitenbach Jan , Tropea Cameron , Roisman Ilia V. 
Published: August 2021

AbstractDrop rebound after collision with a very hot substrate is usually attributed to the Leidenfrost effect, characterized by intensive film boiling in a thin vapour gap between the liquid and substrate. Similarly, drop impact onto a cold superhydrophobic substrate leads to a complete drop rebound, despite partial wetting of the substrate. Here we study the repellent properties of hot smooth hydrophilic substrates in the nucleate boiling, non-Leidenfrost regime and discover that the thermally induced repellency is associated with vapour percolation on the substrate. The wetting structure in the presence of the percolating vapour rivulets is analogous to the Cassie-Baxter wetting mode, which is a necessary condition for the repellency in the isothermal case. The theoretical predictions for the threshold temperature for vapour percolation agree well with the experimental data for drop rebound and correspond to the minimum heat flux when spray cooling.
Cyanate is a low abundance but actively cycled nitrogen compound in soil
Authors: Mooshammer Maria , Wanek Wolfgang , Jones Stephen H. , Richter Andreas , Wagner Michael 
Published: August 2021

AbstractCyanate can serve as a nitrogen and/or carbon source for different microorganisms and as an energy source for autotrophic ammonia oxidizers. However, the extent of cyanate availability and utilisation in terrestrial ecosystems and its role in biogeochemical cycles is poorly known. Here we analyse cyanate concentrations in soils across a range of soil types, land management practices and climates. Soil cyanate concentrations were three orders of magnitude lower than ammonium or nitrate. We determined cyanate consumption in a grassland and rice paddy soil using stable isotope tracer experiments. We find that cyanate turnover was rapid and dominated by biotic processes. We estimated that in-situ cyanate production rates were similar to those associated with urea fertilizer decomposition, a major source of cyanate in the environment. We provide evidence that cyanate is actively turned over in soils and represents a small but continuous nitrogen/energy source for soil microbes.
Xanthine oxidase inhibitor febuxostat reduces atrial fibrillation susceptibility by inhibition of oxidized CaMKII in Dahl salt-sensitive rats
Authors: Xu DongZhu , Murakoshi Nobuyuki , Tajiri Kazuko , Duo Feng , Okabe Yuta , Murakata Yoshiko , Yuan Zixun , Li Siqi , Aonuma Kazuhiro , Song Zonghu , Shimoda Yuzuno , Mori Haruka , Sato Akira , Nogami Akihiko , Aonuma Kazutaka , Ieda Masaki 
Published: August 2021

Abstract
Oxidative stress could be a possible mechanism and a therapeutic target of atrial fibrillation (AF). However, the effects of the xanthine oxidase (XO) inhibition for AF remain to be fully elucidated. We investigated the effects of a novel XO inhibitor febuxostat on AF compared with allopurinol in hypertension rat model. Five-week-old Dahl salt-sensitive rats were fed either low-salt (LS) (0.3% NaCl) or high-salt (HS) (8% NaCl) diet. After 4 weeks of diet, HS diet rats were divided into three groups: orally administered to vehicle (HS-C), febuxostat (5 mg/kg/day) (HS-F), or allopurinol (50 mg/kg/day) (HS-A). After 4 weeks of treatment, systolic blood pressure (SBP) was significantly higher in HS-C than LS, and it was slightly but significantly decreased by treatment with each XO inhibitor. AF duration was significantly prolonged in HS-C compared with LS, and significantly suppressed in both HS-F and HS-A (LS; 5.8 ± 3.5 s, HS-C; 33.9 ± 23.7 s, HS-F; 15.0 ± 14.1 s, HS-A; 20.1 ± 11.9 s: P&lt;0.05). Ca2+ spark frequency was obviously increased in HS-C rats and reduced in the XO inhibitor-treated rats, especially in HS-F group. Western blotting revealed that the atrial expression levels of Met281/282-oxidized Ca2+/Calmodulin-dependent kinase II (CaMKII) and Ser2814-phosphorylated ryanodine receptor 2 were significantly increased in HS-C, and those were suppressed in HS-F and HS-A. Decreased expression of gap junction protein connexin 40 in HS-C was partially restored by treatment with each XO inhibitor. In conclusion, XO inhibitor febuxostat, as well as allopurinol, could reduce hypertension-related increase in AF perpetuation by restoring Ca2+ handling and gap junction.
Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil
Authors: Dubrovskaya Irina , Gerasimenko Evgeniy , Slobodyanik Margarita , Sonin Sergey 
Published: August 2021

Squalene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin brief. Sunflower oil distillation cuts have been selected as the subject of the study, since they area promising secondary raw material for the industrial squalene production. The methods of sample preparation and quantification of squalene in sunflower oil distillation cuts applying gas chromatography in combination with mass spectrometry have been adapted.
The aim of the studyis to create an integrated approach to determining the qualitative and quantitative content of squalene in distillation cuts of vegetable oils. To achieve the goal of the study, the following tasks have been solved:

– Amethod of sample preparation of distillation cuts for determination of squalene has been adapted;
– A method of qualitative and quantitative determination of squalene in distillation cuts has been modified.
As a result of this study, a technique for sample preparation of distillation cuts was proposed as well as a method for the qualitative and quantitative (absolute calibrationmethod) determination of squalene in distillation cuts of sunflower oil. To implement the technique, a Kristall 5000 gas chromatograph equipped with a mass spectrometric detector was used. Squalene and background components were recorded using the NIST 11 mass spectral database.
Trauer, eine bisher wenig beachtete Emotion als Ursache der chronischen Hufrehe
Authors: Torp Christian 
Published: August 2021

ZusammenfassungDie dargestellten Erfahrungen belegen, dass Emotionen, psychische und geistige Faktoren beim Tier wie beim Menschen eine zentrale Rolle in der Krankheitsätiologie spielen und demzufolge in der Therapie Berücksichtigung finden müssen. Wollen wir ein echtes Gesundheitssystem, dann müssen wir all diese Faktoren einbeziehen und den Patienten in den Mittelpunkt unserer Betrachtungen stellen. Die Schulmedizin hat ihren Platz und ihre unanfechtbare Wichtigkeit im Bereich der akuten Erkrankungen. Sprechen wir allerdings von Heilung und wirklicher Gesundheit, dann sollten wir erkennen, dass ein Umdenken im oben dargestellten Sinne erforderlich ist.
Evaluation des Lippstädter Benzo-Checks als
Screening-Instrument für Nebenwirkungen der Langzeiteinnahme von
Benzodiazepinen und Z-Substanzen
Authors: Holzbach Rüdiger , Hunold Petra , Konert Frank 
Published: August 2021

Zusammenfassung
Fragestellung Mit dem Lippstädter Benzo-Check können
(Langzeit-) Anwendern von Benzodiazepinen und Z-Substanzen
überprüfen, ob bei ihnen Nebenwirkungen der Langzeiteinnahme
aufgetreten sind. Diese Studie dient der ersten Überprüfung, ob
der Benzo-Check bei einer Gruppe von Benzodiazepin- und
Z-Substanzen-Abhängigen vor Beginn des Entzuges die entsprechenden
Symptome ausreichend erfasst und andererseits eine depressive Kontrollgruppe
ohne Einnahme der entsprechenden Substanzen unauffällige Werte
erzielt.
Methode 101 stationäre Entzugspatienten mit Abhängigkeit
von Benzodiazepinen bzw. Z-Substanzen und 154 tagesklinische Patienten ohne die
Einnahme von Benzodiazepinen oder Z-Substanzen füllten zu Beginn der
jeweiligen Behandlung den Lippstädter Benzo-Check aus. Die Gesamtscores
bzw. die Subscores „Abhängigkeit“ wurden zwischen beiden
Gruppen verglichen.
Ergebnisse Die Mittelwerte der Gesamtscores der Benzo-Checks in den beiden
Kollektiven unterscheiden sich hochsignifikant, trotzdem sind rund die
Hälfte der depressiven Patienten im untersten von drei Warnbereichen
– „Die von Ihnen angegebenen Symptome kommen wahrscheinlich von
der Langzeiteinnahme der Medikamente“.Allerdings können die Items „Schlafstörung“,
„Heimlichkeit“, „Wirkverlust“,
„Fixierung auf Medikation“ und
„Indikationserweiterung“ zu einem Subscore
(„Abhängigkeits-Score“) zusammengefasst werden, der eine
hohe Trennschärfe für einen Cut-off-Wert von<5 Punkten
erreicht, da 100% der depressiven Patienten und lediglich 10%
der Entzugs-Patienten darunter liegen.
Schlussfolgerungen Die Ergebnisse dieser ersten Studie mit dem
Lippstädter Benzo-Check als Screening-Instrument für
unerwünschte Folgen einer Langzeiteinnahme von Benzodiazepinen oder
Z-Substanzen sind ermutigend, trotz methodischer Limitierungen und der
Notwendigkeit für weitere Untersuchungen.
Effect of Quetiapine, from Low to High Dose, on Weight and Metabolic Traits: Results from a Prospective Cohort Study
Authors: Dubath Céline , Piras Marianna , Gholam Mehdi , Laaboub Nermine , Grosu Claire , Sentissi Othman , Gamma Franziska , Solida Alessandra , von Gunten Armin , Conus Philippe , Eap Chin B. 
Published: August 2021

Abstract
Introduction The atypical antipsychotic quetiapine is known to induce weight gain and other metabolic complications. The underlying mechanisms are multifactorial and poorly understood with almost no information on the effect of dosage. Concerns were thus raised with the rise in low-dose quetiapine off-label prescription (i. e.,<150 mg/day).
Methods In this study, we evaluated the influence of quetiapine dose for 474 patients included in PsyMetab and PsyClin studies on weight and metabolic parameter evolution. Weight, blood pressure, lipid, and glucose profiles were evaluated during a follow-up period of 3 months after treatment initiation.
Results Significant dose-dependent metabolic alterations were observed. The daily dose was found to influence weight gain and increase the risk of undergoing clinically relevant weight gain (≥7% from baseline). It was also associated with a change in plasma levels of cholesterol (total cholesterol, LDL cholesterol, and HDL cholesterol) as well as with increased odds of developing hypertriglyceridemia, as well as total and LDL hypercholesterolemia. No impact of a dose increase on blood pressure and plasma glucose level was observed.
Discussion The dose-dependent effect highlighted for weight gain and lipid alterations emphasizes the importance of prescribing the minimal effective dose. However, as the effect size of a dose increase on metabolic worsening is low, the potential harm of low-dose quetiapine should not be dismissed. Prescriptions must be carefully evaluated and regularly questioned in light of side effect onset.
Thrombotische Mikroangiopathie (TMA) nach Genersatztherapie (GRT) bei spinaler Muskelatrophie: Ein Fallbericht und Therapieoption
Authors: Witte Damian , Hartmann Hans , Drube Jens , Haffner Dieter , Illsinger Sabine 
Published: August 2021

ZUSAMMENFASSUNG
Einleitung Die 5q-assoziierte spinale Muskelatrophie ist eine der schwersten und häufigsten genetischen Erkrankungen. In den letzten Jahren wurden innovative Therapiemethoden entwickelt, die auf der Modifikation des SMN2-Gens, z.B. mittels Spleißmodifikatoren oder dem Ersatz des geschädigten SMN1-Gens basieren (Genersatztherapie, GRT). Es ist bekannt, dass die GRT von Off-Target-Effekten wie einer vorübergehenden Erhöhung der Leber- und Herzenzyme begleitet wird, die in der Regel keine ernsthafte klinische Relevanz haben. Wir berichten über ein 4 Jahre altes Mädchen, das nach GRT bei 5q- SMA eine TMA entwickelte.
Fallzusammenfassung Ein 4 Jahre altes Mädchen bildete 7 Tage nach einer GRT mit Onasemnogene-Abeparvovec eine TMA mit hämolytischer Anämie, Thrombozytopenie und Nierenversagen. Letzteres war gekennzeichnet durch eine Kreatinin-Erhöhung, Oligurie, Hypertonie, Protein- und Hämaturie und Ödeme. Nach Therapie mit Eculizumab und Gabe von Antihypertensiva normalisierten sich die hämolytische Aktivität, die Thrombozytenzahl, die Nierenfunktion als auch der Blutdruck innerhalb einer Woche.
Empfehlung und Schlussfolgerung Die Indikation zur Plasmapherese, Hämodialyse oder Eculizumab-Therapie muss möglicherweise schnell gestellt werden. Bei engmaschiger Überwachung der Laborparameter kann durch eine adäquate Therapie ein gutes Outcome erreicht werden. Daher sollte die GRT nur in Zentren durchgeführt werden, die neben der neuropädiatrischen Expertise auch über eine pädiatrische Nephrologie verfügen.
Uso de guia tridimensional personalizado no preparo do orifício do pedículo piloto em deformidades da coluna vertebral
Authors: Teixeira Kelsen de Oliveira , Matos Thiago Dantas , Fleury Rodrigo Barra Caiado , Costa Herton Rodrigo Tavares , Defino Helton Luiz Aparecido 
Published: August 2021

Resumo
Objetivo O presente estudo teve como objetivo desenvolver e avaliar a utilização de guias personalizadas em pacientes submetidos a cirurgia para correção de deformidades vertebrais com sistema de fixação pedicular.
Métodos Quatro pacientes com deformidade espinhal (três casos de escoliose idiopática e um caso de cifoescoliose congênita) foram submetidos a tratamento cirúrgico corretivo com sistema de fixação pedicular. Protótipos de guias tridimensionais foram desenvolvidos e avaliados quanto à viabilidade técnica, precisão e exposição à radiação.
Resultados O presente estudo incluiu 85 pedículos vertebrais submetidos à inserção de parafusos pediculares na coluna torácica (65,8%) e na coluna lombar (34,2%). A viabilidade técnica foi positiva em 46 pedículos vertebrais (54,1%), sendo 25 torácicos (54%) e 21 lombares (46%). A viabilidade técnica foi negativa em 39 pedículos (45,9%), sendo 31 torácicos (79,5%) e 8 lombares (20,5%). Quanto à precisão, 36 parafusos foram centralizados (78,2%), sendo 17 na coluna torácica (36,9%) e 19 na coluna lombar (41,3%). O mau posicionamento foi observado em 10 parafusos (21,7%), sendo 8 na coluna torácica (17,4%) e 2 na coluna lombar (4,3%). A radiação média registrada nos procedimentos cirúrgicos foi de 5,17 ± 0,72 mSv, e o tempo total de uso da fluoroscopia em cada cirurgia variou de 180,3 a 207,2 segundos.
Conclusão Os protótipos de guias personalizadas permitiram o preparo seguro do orifício piloto nos pedículos vertebrais em casos de deformidade, com maior precisão e menor exposição intraoperatória à radiação.
Paquimeningite hipertrófica idiopática espinhal: Relato de caso
Authors: Araújo Melo Lucas Lodomiro , Daher Murilo Tavares , Gonçalves Marcus Vinícius Magno , Freitas Marianne Borges 
Published: August 2021

ResumoA paquimeningite hipertrófica idiopática é uma causa rara de sintomas neurológicos apresentando mielopatia por compressão da medula espinhal. Relatamos um caso de paquimeningite com manifestação primária de tetraparesia após trauma de baixa energia e recorrência dos sintomas de mielopatia 5 anos após a cirurgia. A paciente, uma mulher de 19 anos, foi submetida a extensa investigação sem evidências de qualquer doença de base. Uma biópsia da meninge revelou processo inflamatório inespecífico com extensa fibrose da dura máter, também visualizado no período perioperatório. Esses achados, associados à exclusão de outras causas, sugerem o diagnóstico de paquimeningite hipertrófica idiopática.