bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of New In Vivo TonB-FepA Rendezvous Sites
Authors: Postle Kathleen , Kho Kelvin , Gresock Michael , Ghosh Joydeep , Larsen Ray 
Published: December 2021

ABSTRACTThe TonB system of Gram-negative bacteria uses the protonmotive force of the cytoplasmic membrane to energize active transport of large or scarce nutrients across the outer membrane by means of customized beta-barrels known as TonB-dependent transporters (TBDTs). The lumen of each TBDT is occluded by an amino-terminal domain, called the cork, which must be displaced for transport of nutrients or translocation of the large protein toxins that parasitize the system. A complex of cytoplasmic membrane proteins consisting of TonB, ExbB and ExbD harnesses the protonmotive force that TonB transmits to the TBDT. The specifics of this energy transformation are a source of continuing interest. The amino terminal domain of a TBDT contains a region called the TonB box, that is essential for the reception of energy from TonB. This domain is the only identified site of in vivo interaction between the TBDT and TonB, occurring through a non-essential region centered on TonB residue Q160. Because TonB binds to TBDTs whether or not it is active or even intact, the mechanism and extent of cork movement in vivo has been challenging to discover. In this study, we used in vivo disulfide crosslinking between eight engineered Cys residues in Escherichia coli TonB and 42 Cys substitutions in the TBDT FepA, including the TonB box, to identify novel sites of interaction in vivo. The TonB Cys substitutions in the core of an essential carboxy terminal amphipathic helix (residues 199-216) were compared to TonB Q160C interactions. Functionality of the in vivo interactions was established when the presence of the inactive TonB H20A mutation inhibited them. A previously unknown functional interaction between the hydrophilic face of the amphipathic helix and the FepA TonB box was identified. Interaction of Q160C with the FepA TonB box appeared to be less functionally important. The two different parts of TonB also differed in their interactions with the FepA cork and barrel turns. While the TonB amphipathic helix Cys residues interacted only with Cys residues on the periplasmic face of the FepA cork, TonB Q160C interacted with buried Cys substitutions within the FepA cork, the first such interactions seen with any TBDT. Both sets of interactions required active TonB. Taken together, these data suggest a model where the amphipathic helix binds to the TonB box, causing the mechanically weak domain of the FepA cork to dip sufficiently into the periplasmic space for interaction with the TonB Q160 region, which is an interaction that does not occur if the TonB box is deleted. The TonB amphipathic helix also interacted with periplasmic turns between FepA β-strands in vivo supporting a surveillance mechanism where TonB searched for TBDTs on the periplasmic face of the outer membrane.
Homestead reduction, economic agglomeration and rural economic development: evidence from Shanghai, China
Authors: Liu Hongmei , Li Guoxiang , Wang Keqiang 
Published: December 2021

PurposeThe contradiction of construction land in economically developed regions is becoming more prominent, and the scale of construction land in some large cities is close to the ceiling. Therefore, China implemented the policy of construction land reduction in 2014. The main objective is to optimize the stock of homesteads and then help to realize rural revitalization by transferring land indexes across regions. Shanghai took the lead in implementing the reduction policy in 2014, for which reduction acceptance data are available. Thus, this paper evaluates the impact of homestead reduction on rural economic development based on data from towns in Shanghai.Design/methodology/approachThis paper uses the difference-in-difference (DID) model to analyze the policy effects of homestead reduction on rural residents' income and industrial integration development. Using economic agglomeration (EA) as a mediating variable, the authors explore how homestead reduction (HR) promotes EA to drive rural economic development and analyze the impact of geographic location and government investment.FindingsHR significantly promotes rural economic development and shows a significant cumulative effect. In the long run, HR can improve rural residents' income and promote industrial integration by promoting EA. The positive effect of HR and EA in suburban regions on industrial integration development is gradually increasing. However, the incentive effect on rural residents' income is weakening. The positive mediating effect of EA is significantly higher in regions with low government investment than in regions with high government investment.Originality/valueThis paper contributes to testing the impact of HR policy on rural economic development and can provide a reference for other regions aiming to implement reduction policy.
Twenty-five years of career growth literature: a review and research agenda
Authors: Modem Roopa , Lakshminarayanan Sethumadhavan , Pillai Rajasekharan , Prabhu Nandan 
Published: December 2021

Purpose
The dynamic career scenario and the significant change from traditionally placing careers in employers’ hands to self-managing one’s careers have sparked profound scholarly interest in career growth. This paper aims to analyze quadricentennial literature on career growth published during the years 1995 through 2020. In this paper, through bibliometric and integrative reviews, the authors address the two critical questions, “what do we know?” and “where should we go?” While the bibliometric review examines the evolution and trends, the integrative review examines the dynamics of conceptual frameworks, primary research foci, research context and quality in research designs and methods.

Design/methodology/approach
The study adopted preferred reporting items for systematic reviews and meta-analyses guidelines to identify the papers for this review. The data set comprised 102 papers and 60 papers for bibliometric and integrative review, respectively. “CiteSpace” is used for the bibliometric analysis and the template designed by the authors is used for the integrative review.

Findings
The results present conceptual clarification for career growth and its related constructs. The bibliometric analysis findings highlight the extensive research on career growth followed by organizational career growth and career growth opportunities. The findings also show that seven of the top 10 productive authors are from China. This study also identifies theoretical, conceptual and methodological opportunities and provides recommendations intended to further research engagements across the different aspects of career growth.

Originality/value
To the best of the authors’ knowledge, this is the first study to introduce bibliometric analysis into career growth literature. This research adopts an integrative review approach and offers insights into career growth literature.
A structured literature review on Big Data for customer relationship management (CRM): toward a future agenda in international marketing
Authors: Del Vecchio Pasquale , Mele Gioconda , Siachou Evangelia , Schito Gloria 
Published: December 2021

PurposeThis paper aims to advance the international marketing debate by presenting the results of a structured literature review (SLR) focusing on Big Data implementation in customer relationship management (CRM) strategizing. It outlines past and present literature and frames a future research agenda.Design/methodology/approachThe research analyzes papers published in journals from 2013 to 2020, deriving significant insights about Big Data applications in CRM. A sample of 48 articles indexed at Scopus was preliminarily submitted for bibliometric analysis. Finally, 46 papers were analyzed with content and a bibliometric analysis to identify areas of thematic specializations.FindingsThe paper presents a conceptual multilevel framework demonstrating areas of specialization emerging from the literature. The framework is built around four coordinated sequences of actions relevant to “why,” “what,” “who” and “how” Big Data is implemented in CRM strategies, thus supporting the conception and implementation of an internationalization marketing strategy.Research limitations/implicationsImplications for the development of the future research agenda on international marketing arise from the comprehension of Big Data in CRM strategy.Originality/valueThe paper provides a comprehensive SLR of the articles dealing with models and processes of Big Data for CRM from an international marketing perspective. Despite these issues' relevance and the increasing literature focused on them, research in this area is still fragmented and underexplored, requiring more systematic and holistic studies.
COVID-19 and food security as catalyst of conflict among rural households in Nigeria: a study of Ilaje community, Ondo state
Authors: Adebayo Tosin Seun , Oluwamayowa Logo 
Published: December 2021

Purpose
The compulsory lockdown issued by the Nigeria Government to curb the spread of the virus has undoubtedly negatively affected the livelihoods of households. As a result, many have lost their sources of income and have no other means for survival. It is important to note that it has the potential of triggering violent conflicts and civil unrest as a popular African adage goes as thus: “A hungry man is an angry man”. Unfortunately, little attention has been given, neither has extant literature adequately covered the impact of the coronavirus (COVID-19) outbreak as it directly affects households in a rural community in Nigeria.

Design/methodology/approach
This study proceeded from on both a qualitative and quantitative method and adopted a mixed-method and case study research design. Data for this study was collected through primary and secondary data. Primary data for this study was collected through a questionnaire and focus group discussions (FGD). The questionnaire used for this study was a validated instrument designed by the US Department of Agriculture (USDA) to measure food security amongst households. The USDA household’s instrument was used and then modified to access the food security status of households during the lockdown.

Findings
Findings from this study revealed that households, in general, were categorised as having “very low food security” during the lockdown as they testified to all the food insecure conditions. In general, the food intake of household members reported irregular eating patterns as the household lacked the money to purchase food. This study further indicated that a high number of respondents see violence as a means of attaining food. Extreme volatility in their inability to access food as a result of food shortages and the inability to benefit from government palliatives has been found to trigger incidents of conflict and malnutrition on households.

Research limitations/implications
This method was sampling method was adopted because of the compulsory and constitutional lockdown imposed by the government to curb the spread of the novel COVID-19 which limited the movement of the researcher to other households in the community which proved to be limited during the time this research was carried limiting the scope of the sample size.

Originality/value
This research adopted mixed-method research and was written by the researchers who did the study. This paper was conducted during the mandatory lockdown issued by the Federal Government in Nigeria with to understand the impact of the mandatory lockdown on rural households using Ilaje local of Ondo state as a case study while establishing a link between food security and conflict.
Efficacy of a combination of paclitaxel and radiation therapy against cutaneous angiosarcoma: A single‐institution retrospective study of 21 cases
Authors: Seo Takashi , Kitamura Shinya , Yanagi Teruki , Ujiie Hideyuki 
Published: December 2021

AbstractCutaneous angiosarcoma (CAS) is a rare malignant tumor with a poor prognosis for which neither basic research on molecular pathomechanisms nor clinical prospective studies have progressed. A retrospective study of 28 CAS cases reported that chemoradiotherapy with taxanes was superior to conventional surgery. Since that time, chemoradiotherapy with paclitaxel (PTX + RT) has become a standard treatment. In this paper, we retrospectively investigate 21 cases of CAS that had been treated in the Department of Dermatology at Hokkaido University Hospital. Patients initially treated with PTX + RT followed by maintenance taxane chemotherapy showed better prognosis in overall survival (OS) and progression‐free survival (PFS) (median OS, 28 months; median PFS, 12 months) compared to others (median OS, 10 months; median PFS, 5 months) (OS and PFS: p < 0.05, log–rank test). Our results are consistent with those of a previous study that suggested that PTX + RT followed by maintenance taxane chemotherapy is a better therapeutic option than other interventions, including surgery.
Transient ascending ST‐segment depression and widening of the S wave in 3‐channel Holter monitoring—A sign of dromotropic disturbance in the right ventricular outflow tract in the Brugada syndrome: A report of five cases
Authors: de Andrade Antonio Thomaz , Barbosa‐Barros Raimundo , Nikus Kjell , Raimundo Rodrigo D. , de Abreu Luiz C. , Sacilotto Luciana , Darriuex Francisco C. C. , Yanowitz Frank G. , Brugada Pedro , Pérez‐Riera Andrés Ricardo 
Published: December 2021

AbstractBackgroundBrugada syndrome (BrS) is somewhat a challenging diagnosis, due to its dynamic pattern. One of the aspects of this disease is a significant conduction disorder located in the right ventricular outflow tract (RVOT), which can be explained as a consequence of low expression of Connexin‐43. This decreased conduction speed is responsible for the typical electrocardiographic pattern. Opposite leads located preferably in inferior leads of the electrocardiogram may show a deep and widened S wave associated with ascending ST segment depression. Holter monitoring electrocardiographic (ECG) aspects is still a new frontier of knowledge in BrS, especially in intermittent clinical presentations.MethodsWe describe, as an exploratory analysis, five case series of intermittent type 1 BrS to demonstrate the appearance of ascending ST segment depression and widening of the S wave, during 3‐channel 24h‐Holter monitoring (C1, C2 and C3) with bipolar leads.ResultsIn the five cases described, the ST segment depression was observed mainly in C2, but in some cases also in C1 and C3. Only case 1 presented concomitant intermittent elevation of the ST segment in C1. All cases were intermittent.ConclusionThe recognition of an ECG pattern with ascending ST‐segment depression and widening of the S wave in 3‐channel Holter described in this case series should raise a suspicion of the BrS and suggests the counterpart of a dromotropic disturbance registered in the RVOT and/or reciprocal changes.
Comparative study on electrocardiograms and serological examinations of acute pulmonary embolism and acute non‐ST elevation myocardial infarction
Authors: He Zhihong , Bi Wenmiao , Lang Zhe , Zhen Yanjie , Jin Ying , Liu Hongjuan , Li Dongfu , Hu Xiaoning , Li Huanling 
Published: December 2021

AbstractBackgroundThe aim of this study was to investigate the value of electrocardiograms (ECGs) and serological examinations in the differential diagnosis of acute pulmonary embolism (APE) and acute non‐ST elevation myocardial infarction (NSTEMI) in order to reduce the rate of clinical misdiagnosis.MethodsThe clinical data of 37 patients with APE and 103 patients with NSTEMI admitted to our hospital were retrospectively analyzed. The differences in the clinical manifestations, ECGs, myocardial zymograms, D‐dimers, and troponin (cTn) of the two groups were compared.ResultsIn the patients with APE, the main symptom—found in 25 cases (67.56%)—was dyspnea, while in the patients with NSTEMI, the main symptom—found in 52 cases (50.49%)—was chest tightness. The incidences of sinus tachycardia and SIQIIITIIIin the group of patients with APE were higher than in the group of patients with NSTEMI, and the difference was statistically significant (p < .05). There was no statistical significance in the difference of aspartate aminotransferase and lactate dehydrogenase (LDH) in the two groups (p > .05), although there was a statistically significant difference of creatine kinase (CK) and the creatine kinase isoenzyme‐MB (CK‐MB) in the two groups (p < .05). The levels of D‐dimers and cTn were increased in both groups, but the level of D‐dimers in the group of patients with APE was higher than that in the group of patients with NSTEMI.ConclusionWith the occurrence of clinical manifestations like dyspnea, chest tightness, chest pain, and palpitation of unknown causes, the possibility of APE and NSTEMI should be considered.
Petrographical and geochemical criteria for a chronology of Roman mortars between the first century bce and the second century ce: The Curia of Pompey the Great
Authors: Marra Fabrizio , D'Ambrosio Ersilia , Gaeta Mario , Monterroso‐Checa Antonio 
Published: December 2021

AbstractThirteen samples of mortar collected from different masonry structures of the Curia of Pompey the Great and from three mixtilinear basins located within the Sacred Area of Largo Argentina of Rome were studied. Despite the use of the same volcanic deposit, known as ‘Pozzolane Rosse’, to produce the fine aggregate in all these mortars, it was possible to highlight some distinctive features through the combination of geochemical analyses on selected trace elements and petrographic analysis under an optical microscope, allowing the three groups of mortars to be distinguished. These types of mortars reflect a perfect coincidence between the diversity of the volcanic materials used and the different construction phases identified and documented by the analysis of the stratigraphic units: a first construction phase of Pompeian age, a second phase of Augustan age and a final phase in the medieval period. Furthermore, it was possible to ascertain two phases of construction of the basins, the second coeval with the interventions of the Augustan period. Finally, this study increases knowledge about the methods of exploitation and selection of volcanic materials used to produce mortars in the Roman period, identifying additional elements useful to establish their origin and chronology of use.
Flow cytometric minimal residual disease assessment in B‐cell precursor acute lymphoblastic leukaemia patients treated with CD19‐targeted therapies — a EuroFlow study
Authors: Verbeek Martijn W. C. , Buracchi Chiara , Laqua Anna , Nierkens Stefan , Sedek Lukasz , Flores‐Montero Juan , Hofmans Mattias , Sobral de Costa Elaine , Nováková Michaela , Mejstrikova Ester , Barrena Susana , Kohlscheen Saskia , Szczepanowski Monika , Kulis Jan , Oliveira Elen , Jugooa Romana , de Jong Anja X. , Szczepanski Tomasz , Philippé Jan , van Dongen Jacques J. M. , Orfao Alberto , Brüggemann Monika , Gaipa Giuseppe , van der Velden Vincent H. J. 
Published: December 2021

SummaryThe standardized EuroFlow protocol, including CD19 as primary B‐cell marker, enables highly sensitive and reliable minimal residual disease (MRD) assessment in B‐cell precursor acute lymphoblastic leukaemia (BCP‐ALL) patients treated with chemotherapy. We developed and validated an alternative gating strategy allowing reliable MRD analysis in BCP‐ALL patients treated with CD19‐targeting therapies. Concordant data were obtained in 92% of targeted therapy patients who remained CD19‐positive, whereas this was 81% in patients that became (partially) CD19‐negative. Nevertheless, in both groups median MRD values showed excellent correlation with the original MRD data, indicating that, despite higher interlaboratory variation, the overall MRD analysis was correct.
Nivolumab‐induced localized genital bullous pemphigoid in a 60‐year‐old male
Authors: Alvarado Savannah M. , Weston Gillian , Murphy Michael J. , Stewart Campbell L. 
Published: December 2021

AbstractA 60‐year‐old man with metastatic renal cell carcinoma presented with a 6‐month history of a pruritic, exquisitely painful genital eruption appearing 3 months after initiation of nivolumab. Examination demonstrated a poorly defined, lichenified scrotal plaque studded with erosions, yellow crust, and tense vesicles. There was no other lesion on the body or mucosae. Histopathology revealed a subepidermal blister with a mixed lymphocytic, neutrophilic, and eosinophilic infiltrate. Direct immunofluorescence of perilesional skin demonstrated subclinical blister and linear/fibrillary patchy IgG and IgA along the dermoepidermal junction. Bullous pemphigoid (BP) serologies revealed normal IgG BP230 antibodies and minimally elevated IgG BP180 antibodies. Indirect immunofluorescence revealed positive IgG at the basement membrane (“epidermal pattern”) in human split skin and monkey esophagus substrates; no IgA antibodies were detected. The patient was diagnosed with nivolumab‐induced localized genital BP (LGBP). BP is a reported adverse effect of immune checkpoint inhibitors including nivolumab; however, cases are typically generalized. LGBP is a rare BP variant typically presenting in children and females; there are few reports of LGBP in adult males. We report a novel case of nivolumab‐induced LGBP with unique histopathologic and clinical challenges. LGBP should be considered in patients on immune checkpoint inhibitor therapy with bullous genital eruptions.
The case for a diversion option to trauma‐informed community‐based primary mental health services for young people following early encounters with police
Authors: Baker David , Rice Simon , Purcell Rosemary 
Published: December 2021

AbstractAimThere is a convergence between the peak age of onset of mental illness, offending behaviour and associated risk of crime victimization. This study compared self‐reported childhood maltreatment and mental health outcomes among a cohort of help‐seeking young people who disclosed either a prior criminal charge, crime victimization, or both, to those who did not report any such experiences. The outcomes may inform the development of a diversion option for young people at a first or early encounter with police.MethodsThe Transitions Study (N = 802; mean age = 18.3 years; 66% female) baseline and 12‐month follow‐up data were analysed in relation to childhood maltreatment (Childhood Trauma Questionnaire) and changes in mental health (Kessler Psychological Distress Scale and Social and Occupational Functioning Assessment Scale) among young people attending headspace centres in Australia. Outcomes were compared between those young people reporting a prior criminal charge and/or crime victimization and those who did not.ResultsYoung people who reported a prior criminal charge or crime victimization reported higher frequency and severity of childhood maltreatment, than those who did not. Both groups reported a reduction in psychological distress following youth mental health service access at 12‐month follow‐up, however functional improvement was only evident for young people who did not report a prior criminal charge or crime victimization.ConclusionsHigher rates of reported childhood maltreatment and reduced psychological distress following service access supports trialling a diversion option to a trauma‐informed community‐based primary mental health service for young people following an early encounter with police.