bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Telomere Length and Molecular Characterization of TERT Gene Promoter in Periampullary Carcinomas
Authors: Gregório Cleandra , Thakur Shefali , Rivero Raquel Camara , Machado Simone Márcia dos Santos , Cuenin Cyrille , Carreira Christine , White Valerie , Cree Ian A , Vukojevic Katarina , Durdov Merica Glavina , Osvaldt Alessandro Bersch , Ashton-Prolla Patricia , Herceg Zdenko , Talukdar Fazlur Rahman 
Published: December 2021

Abstract
Genetic and epigenetic alterations of the telomere maintenance machinery like telomere length and telomerase reverse transcriptase (encoded by TERT gene) are reported in several human malignancies. However, there is limited knowledge on the status of the telomere machinery in periampullary carcinomas (PAC) that are rare and heterogeneous groups of cancers arising from different anatomic sites around the ampulla of Vater. In the current study, we investigated the relative telomere length (RTL) and the most frequent genetic and epigenetic alterations in the TERT promoter in PAC (n=20) and compared with tumor-adjacent nonpathological duodenum (NDu; n=16). We found shorter RTLs (1.27 vs 1.33, P=0.01) and lower TERT protein expression (p=0.04) in PAC tissues as compared to the NDu. Although we did not find any mutation at two reactivating hotspot mutation sites of the TERT promoter, we detected polymorphism in 55% (11/20) of the cases at rs2853669 (T>C). Also, we found a hypermethylated region in the TERT promoter of PACs consisting of four CpGs (cg10896616 with ∆β 7%; cg02545192 with ∆β 9%; cg03323598 with ∆β 19%; and cg07285213 with ∆β 15%). In conclusion, we identified shorter telomeres with DNA hypermethylation in the TERT promoter region and lower TERT protein expression in PAC tissues. Further studies with a larger sample size are necessary to substantiate these results.
Identification the key genes between polycystic ovary syndrome and Non-Alcoholic Fatty Liver Disease
Authors: Yifu Pu 
Published: December 2021

Abstract
Purpose Many studies show correlation between polycystic ovary syndrome(PCOS) and non-alcoholic fatty liver disease(NAFLD),but the underlying pathogenic genes are not clear. This study by using the bioinformatics method aims to search the key genes involved in these 2 diseases.
Methods The Gene Expression Omnibus (GEO) datasets coming from the GEO database GSE63067 -NAFLD patients and healthy controls, and GSE34526 -PCOS patients and normal controls, are downloaded. Differentially expressed genes (DEGs) of 2 diseases datasets and the common genes are obtained. After GO and KEGG enrichment analyses of common genes are performed. To find the key genes between NAFLD and PCOS, a protein–protein interaction (PPI) network is carried out. In addition, the diagnostic value of key genes in PCOS is analyzed.
Results According to NAFLD and PCOS downloaded datasets,34 common genes,21 key genes,15 GO terms and 4 KEGG pathways are obtained. Further,based on the top 6 key genes,the corresponding area under the curve (AUC) by constructing ROC curves in the PCOS is 0.909 (95% CI, 0.775–1.000).
Conclusions The study identify some key genes in the occurrence and progression between NAFLD and PCOS. In the future,to verify our results,it need experimental and clinical research.
Child-centred pandemic decisions: how the Precautionary Principle can generate better risk assessment in an era of uncertainty
Authors: Kvalsvig Amanda , Russell Jin , Timu-Parata Carmen , Baker Michael G. 
Published: December 2021

Abstract
Key messagesRisk assessment for children has been a polarising issue during the Covid-19 pandemic. Governments around the world are preparing to ‘open up’ before risks to children are fully quantified, with unknown implications for their long-term health.Applying the Precautionary Principle to child health requires decision makers to 1) take preventive action until risks are better understood; 2) ensure that the burden of proof rests with proponents of risk; 3) explore alternatives to the risk; and 4) use participatory approaches to decision-making.Policies relating to children must be centred on the rights and wellbeing of children. We provide a framework for comprehensive Health Impact Assessments to ensure that direct and indirect impacts upon children are taken into account in major policy decisions.Elimination strategies offer an integrated approach to the protection of children’s wellbeing, the wellbeing of the population as a whole, and health equity. Where countries are transitioning away from elimination, a tight suppression approach is preferable to loose suppression or mitigation.
Structural-bioinformatics analysis of SARS-CoV-2 variants reveals higher hACE2 receptor binding affinity for Omicron B.1.1.529 spike RBD compared to wild-type reference.
Authors: Durmaz Vedat , Köchl Katharina , Singh Amit , Hetmann Michael , Parigger Lena , Krassnigg Andreas , Nutz Daniel , Korsunsky Alexander , König Centina , Chang Lee , Krebs Marius , Bassetto Riccardo , Pavkov-Keller Tea , Resch Verena , Gruber Karl , Steinkellner Georg , Gruber Christian C. 
Published: December 2021

Abstract
To date, more than 263 million people have been infected with SARS-CoV-2 during the COVID-19 pandemic. In many countries, the global spread came in several pandemic waves characterized by the emergence of new SARS-CoV-2 variants. Here, we report on a sequence- and structural-bioinformatics analysis by which we estimate the impact of amino acid exchanges on the affinity of the SARS-CoV-2 spike receptor-binding domain (RBD) to the human receptor hACE2. This is carried out by qualitative electrostatics and hydrophobicity analysis as well as through molecular dynamics simulations used for the development of a highly accurate linear interaction energy (LIE) binding affinity model that was calibrated on a large set of experimental binding energies. For the newest variant of concern (VOC), B.1.1.529 Omicron, our Halo difference point cloud studies reveal the largest impact on the RBD binding interface compared to any other VOC. Moreover, according to our LIE model, Omicron achieved a substantially higher ACE2 binding affinity than the wild-type and in particular the highest among all VOCs except for Alpha and therefore requires special attention and monitoring. Using this prediction model we provide early structural insight and binding properties before experimentally determined complex structures and binding affinity data become available in the upcoming months.
The Study of Multilayer Graphene Membrane Performance in O2 Purification Process: Molecular Dynamics Simulation
Authors: Jafari Mostafa , Sabetvand Roozbeh 
Published: December 2021

Abstract
We use molecular dynamics (MD) method to describe the atomic behavior of Graphene nanostructure for Oxygen molecules (O2) separation from Carbon dioxide (CO2) molecules. Technically, for the simulation of graphene-based membrane and O2-CO2 gas mixture, we used Tersoff and DREIDING force fields, respectively. The result of equilibrium process of these structures indicated the good stability of them. Physically, this behavior arises from the appropriate MD simulation settings. Furthermore, to describe the purification performance of graphene-based membrane, we report some physical parameters such as purification value, impurity rate, and permeability of membrane after atomic filtering process. Numerically, by defined membranes optimization, the purification value of them reach to 97.31%. Also, by using these atomic structures the CO2 impurity which passed from graphene-based membrane reach to zero value.
Engineering and Fermenter Optimization of Fungi GLA Productions in Pichia Pastoris GS115 Using Oil Waste
Authors: Firoozabad Maryam Sadat Mirbagheri , Akhbariyoon Hamidreza 
Published: December 2021

Abstract
γ-Linolenic acid (GLA) is an important n-6 polyunsaturated fatty acid (PUFA) that has received considerable attention in both levels in human and animal feed. GLA is used in many nutritional and medicinal applications such as the treatment of cancer, inflammatory disorders, and diabetes. Currently, plant seed is the main dietary source of GLA that is not enough to utilize on an industrial scale. To generate a sustainable novel source of GLA, the gene of delta-6 desaturase, which is one of the important enzymes in the GLA production pathway was isolated from Mucor rouxii DSM1194 and expressed in Pichia pastoris GS115 by pPICZC vector. The recombinant yeast expressed the GLA up to 19.2% (72 mg/g) of total fatty acids. GLA production of recombinant yeast was studied in fermenter by oil waste along 5 days and results detected 6.3 g / l lipid and 103 mg/g GLA was produced in 72 hours. The present study may provide an opportunity for the development of an alternative host for manufacturing GLA on an industrial scale.
Peran Rumah Aspirasi "TITIS" dalam Mendampingi Perempuan Korban Kekerasan
Authors: Yasmin Yuditya Firdauza , Ashidiq Heharero Tesar 
Published: December 2021

Kekerasan merupakan sebuah mata rantai yang sulit untuk dihapuskan. Fenomena pandemi covid-19 menjadi salah satu katalis dalam tindak kekerasan terhadap perempuan. Jawa Tengah menjadi salah satu provinsi dengan jumlah tertinggi kasus kekerasan terhadap perempuan. Akan tetapi, Kabupaten Kebumen pada tahun 2020 justru mengalami penurunan kasus. Salah satu upaya yang dilakukan oleh pemerintah Kabupaten Kebumen adalah dengan mendirikan Rumah Aspirasi Perempuan “TITIS”. Penelitian ini berusaha untuk menjelaskan lebih jauh lagi terkait peran Rumah Aspirasi Perempuan yang telah didirikan oleh Pemerintah Kabupaten Kebumen dalam mendampingi korban kekerasan dan mendukung upaya pemerintah untuk mengurangi angka kekerasan terhadap perempuan. Penelitian ini menggunakan pendekatan kualitatif. Teknik pengumpulan data yang digunakan adalah wawancara mendalam dan kajian pustaka dengan mengumpulkan data dari berbagai sumber. Rumah aspirasi perempuan titis memiliki tugas pokok dan fungsi melakukan pendampingan terhadap korban-korban kekerasan sekaligus memberikan konseling. Kasus kekerasan berbasis gender dan anak di Kabupaten Kebumen pada tahun 2020 mengalami penurunan dari tahun 2019. Hal ini dikarenakan banyak korban kekerasan dalam rumah tangga (KDRT) tidak melapor kepada pihak layanan sosial atau dalam hal ini Rumah Aspirasi Perempuan “Titis” maupun kepada layanan sosial yang berada dibawah naungan Dispermades P3A Kabupaten Kebumen. Korban kekerasan cenderung untuk langsung menempuh jalur hukum dan melaporkan kasus kekerasan tersebut ke aparat kepolisian setempat. Selain itu, kurangnya pengetahuan masyarakat menjadi salah satu penyebab rendahnya laporan yang masuk ke rumah aspirasi perempuan titis. Oleh sebab itu, rumah aspirasi titis bekerja sama dengan pemerintah desa guna mendapatkan data tambahan terkait kekerasan terhadap perempuan agar dapat memberikan pendampingan korban.
Kata kunci : Kekerasan, Perempuan, Rumah Aspirasi, Kabupaten Kebumen.
KOMPARASI PERKEMBANGAN POTENSI ANAK USIA DINI MENURUT ISLAM DENGAN TEORI KOHLBERG
Authors: Bahri Saiful 
Published: December 2021

Abstrak: Kohlberg sangat memperdulikan perkembangan manusia, sehingga dia mengemukakan tiga tahapan perkembangan potensi manusia, sedangkan Islam sebagai agama yang rahmatan lil ‘alamiin sangat memperdulikan perkembangan manusia, sehingga mengemukakan konsep tentang perkembangan tersebut dalam hadits dan al-Qur’an. Persamaan antara kedua teori tersebut adalah sama-sama mengungkapkan tahapan-tahapan perkembangan anak dan perkembangan potensi anak. Kemudian juga mengungkapkan tahapan pendidikan yang harus dilaksanakan kepada anak supaya anak dapat berkembang sesuai dengan bakat alaminya. Perbedaannya mulai dari perbedaan epistemologi dan juga perbedaan pengembangan konsep fitrah.

Kata kunci: Potensi Anak Usia Dini, Teori Kohlberg
Abstract: Kohlberg really cares about human development, so he put forward three stages of human potential development, while Islam as a religion which is rahmatan lil 'alamin really cares about human development, so he put forward the concept of this development in the hadith and the Qur'an. The similarities between the two theories are that they both reveal the stages of child development and the development of children's potential. Then also reveal the stages of education that must be carried out to children so that children can develop according to their natural talents. The differences start from differences in epistemology and also differences in the development of the concept of fitrah.

Keywords: Early Childhood Potential, Kohlberg's Theory
Incidence of lateral incisor root resorption associated with impacted maxillary canines
Authors: Lipshatz Jeff , Ptasznik Ronnie , Wenig Shmuli 
Published: December 2021

Abstract

Introduction
The aim of this study was to determine the incidence of lateral incisor root resorption associated with impacted maxillary canines and determine predisposing factors that may be used to predict its occurrence.

Methods
Cone beam computerised tomographic images of 133 patients presenting with 186 impacted canines were examined for lateral incisor root resorption. A control sample consisted of 30 lateral incisors on the side of the non-impacted canine. The studied canine-associated variables were gender, type of impaction, location of the canine both meso-distally and vertically and the long axis angulation to the midline. Axial images were primarily used to diagnose resorption.

Results
The estimated percentage of lateral root resorption in the sample was 17% (range 11.8– 23.9%) confirmed at a 95% confidence interval. A significant association was observed between the level of overlap of the canine across the lateral incisor, measured in sectors, and the probability of lateral incisor root resorption. The probability approximately doubled for each additional sector of canine overlap. No other significant association was noted related to all the other variables examined.

Conclusions
The incidence of lateral incisor root resorption associated with impacted maxillary canines was lower in the present study compared with many previous reports. However, resorption remains a common clinical finding. In order to screen for lateral incisor resorption, it is recommended that a cone beam image be prescribed when there is a mesial overlap of an impacted canine across the lateral incisor midline.
Colonialism and Conservation
Authors: LENNOX ROWENA , PROBYN-RAPSEY FIONA 
Published: December 2021

Abstract
This article describes and analyses the Pelorus experiment, a recent restoration project in which dingoes were used to eradicate goats on a Great Barrier Reef Island. Before they were taken to the island, the dingoes were implanted with a poison capsule that was intended to kill them after they had killed the goats; they were both pest and ‘pesticide’ (Mavhunga 2011). Subsequently, freehold land on the island was marketed as a tourism development site. We contextualise the Pelorus Island goat eradication program within the cultural–political history of carceral colonialism in Australia and show how this experiment relates to ideas about the special role that islands play in conservation. We also piece together the story of what happened to the goats and dingoes involved. Our analysis reveals the ways notions of animal pesthood and ecological restoration are co-opted by conservation and tourism interests. The Pelorus experiment illustrates how illusions of idyllic island sanctuaries, which appeal to contemporary tourism tropes of seclusion within a ‘pristine’ environment, are predicated on the violence inherent to, but obfuscated within, settler-colonialist visions of land for the taking and remaking.
First report of root-lesion nematode, Pratylenchus oleae from pistachio in Iran
Authors: Naeini Farhad Saeidi , Taheri Zahra Majd 
Published: December 2021

Abstract
Pistachio, Pistacia vera is one of the most important cash crops in Iran that is scattered in arid and semi-arid regions. During a survey of plant-parasitic nematodes of pistachio in Ardakan city in Yazd Province, a species of root-lesion nematode was isolated and identified by morphological, morphometrical, and molecular methods as Pratylenchus oleae Palomares-Rius et al., 2014. This species was isolated from several pistachio trees rhizosphere regarding to molecular analysis, D2–D3 expansion segments of 28S rRNA was amplified by PCR and sequenced. The sequence was deposited in GenBank (Accession No. MW338666). Along with the related phylogenetic analysis, placed this species in a monophyletic clade with other Pratylenchus oleae isolates, based on Bayesian inference (BI) phylogeny. A PCR reaction with the P. oleae specific primer set produced a 547 bp fragment. This is the first report of P. oleae infecting Pistachio tree in the world.
Record data from Kies distribution and related statistical inferences
Authors: Al-Olaimat Nesreen M. , Bayoud Husam A. , Raqab Mohammad Z. 
Published: December 2021

Abstract
The Kies probability model was proposed as an alternative to the extended Weibull models as it provides a more efficient fit to some real-life data sets in comparison to the aforementioned models. The paper proposes classical and Bayesian inferences for the Kies distribution based on records. Maximum likelihood estimates are studied jointly with asymptotic and bootstrap confidence intervals. Moreover, Bayes estimates, along with credible intervals are discussed assuming squared and LINEX loss functions. The proposed estimation methods have been investigated and compared via simulation studies. A real data set has been analysed for illustrative purposes.
Relationships for moments of the progressively Type-II right censored order statistics from the power Lomax distribution and the associated inference
Authors: Saran Jagdish , Pushkarna Narinder , Sehgal Shikha 
Published: December 2021

Abstract
In this paper, we establish several recurrence relations between single and product moments of progressively Type-II right censored order statistics from the power Lomax distribution. The relations enable the computation of all the single and product moments of progressively Type-II right censored order statistics for all sample sizes n and all censoring schemes (R
1, R
2,..., R

m
), m ≥ n, in a simple recursive manner. The maximum likelihood approach is used for the estimation of the parameters and the reliability characteristic. A Monte Carlo simulation study has been conducted to compare the performance of the estimates for different censoring schemes.
Feasibility analysis of water resources market transaction of water diversion project based on evolutionary game
Authors: Liu Mengkai , Yan Acai 
Published: December 2021

AbstractWater diversion projects realize the change of water rights ownership by diverting water resources, but most of the water diversion projects are dominated by the government in the operation stage, which can't realize complete market-oriented transaction of resources. In this study, the water source area, project management department and users involved in water resources trading of a water diversion project are regarded as stakeholders, and an evolutionary game model is established to analyze the feasibility and restrictive conditions of the free trading of water resources in the water diversion project. The research results show that the three parties can realize the balance and stability of interests (the water source area supplies good water, the project management department supplies sufficient water, users take water according to the supply), which means that the water market transactions are feasible. Meanwhile, it also gives clear restrictions on the water price of the three parties transaction: the project management department water price for purchase is less than on the premise of lower than + , the sale water price is not higher than . The free trading of water resources in the water diversion project under the market mechanism is of great significance for clarifying the water rights and reducing the financial pressure of the government.
Using CFD modelling to study hydraulic flow over labyrinth weirs
Authors: Idrees Anees K. , Al-Ameri Riyadh , Das Subrat 
Published: December 2021

Abstract
The compound labyrinth weir is a new type of labyrinth weir that is considered a good applicable choice for increasing the capacity of discharge. The flow over a compound labyrinth weir is a complex problem because the flow behavior is three-dimensional. The present study aims to simulate the flow over the compound labyrinth weir into the critical regions that cannot be observed when using an experimental test. The computational fluid dynamics (CFD) programme was utilised to implement a sensitive analysis for this purpose and under different flow conditions. The MAPE and RMSE indices were utilised to verify the CFD results with experimental work. The statistical indices of the maximum error ME, RMSE and MAPE were 4.7%, 0.033 and 3.9 respectively. Therefore, the findings showed that there is a good matching between the experimental and CFD results. The CFD results demonstrated that the hydraulics behaviour of the compound labyrinth weir was similar to the oblique and linear weirs in high discharges. The results also confirmed that air cavities and bubbles existed behind the nappe flow in addition to the negative pressure that may occur beneath the nappe when the flow is aerated. Furthermore, the flow was divided into two parts and most streamlines were concentrated over the notches. Moreover, the flow velocity passing through the notches was greater than the flow velocity over the high crest of the compound labyrinth weir.
Construction and Evaluation of Recombinant Chimeric Fibrillin and Elastin Fragment in Human Mesenchymal Stem Cells
Authors: Jeong Eui-Seung , Park Bo-Hyun , Lee Sujin , Jang Jun-Hyeog 
Published: December 2021

Background:
Diverse extracellular matrix (ECM) proteins physically interact with stem
cells and regulate stem cell function. However, the large molecular weight of the natural ECM renders
large-scale fabrication of a similar functional structure challenging.

Objective:
The objective of this study was to construct a low molecular weight and multifunctional
chimeric form of recombinant ECM to stimulate mesenchymal stem cell (MSC) for tissue repair.
We engineered Fibrillin-1PF14 fused to an elastin-like polypeptide to develop a new biomimetic
ECM for stem cell differentiation and investigated whether this recombinant chimeric Fibrillin-Elastin
fragment (rcFE) was effective on human nasal inferior turbinate-derived mesenchymal stem
cells (hTMSCs).

Methods:
hTMSCs were grown in the medium supplemented with rcFE, then the effect of the protein
was confirmed through cell adhesion assay, proliferation assay, and real-time PCR.

Results:
rcFE enhanced the adhesion activity of hTMSCs by 2.7-fold at the optimal concentration,
and the proliferation activity was 2.6-fold higher than that of the control group (non-treatment
rcFE). In addition, when smooth muscle cell differentiation markers were identified by real-time
PCR, Calponin increased about 6-fold, α-actin about 9-fold, and MYH11 about 10-fold compared
to the control group.

Conclusion:
Chimeric rcFE enhanced cellular functions such as cell adhesion, proliferation, and
smooth muscle differentiation of hTMSCs, suggesting that the rcFE can facilitate the induction of
tissue regeneration.