bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epithelial-Mesenchymal Transition-Related lncRNAs And SNAI2 Are Potential Biomarkers in Coronary Artery Disease
Authors: Xu Xiang , Zou Renchao , Liu Xiaoyong , Su Qianqian 
Published: November 2021

Abstract
BackgroundIncreasing evidence suggests that epithelial-mesenchymal transformation (EMT) is critical in the development of inflammatory response, atherosclerosis, and coronary artery disease (CAD). However, landscapes of EMT-related lncRNAs and their target genes have not been fully established in CAD.MethodsLncRNA and mRNA expression profiles obtained from Gene Expression Omnibus (GEO) database were used to identify the differentially expressed mRNAs (DEGs) and lncRNAs (DElncRNAs) between CAD and normal samples. Based on Pearson correlation analysis to identify the EMT-related lncRNAs, the optimal features were identified by receiver operating characteristic (ROC), the least absolute shrinkage and selection operator (LASSO) regression, Support Vector Machine Reverse Feature Elimination (SVM-RFE) algorithms, and logistic regression models were constructed aiming to distinguish CAD from normal samples. The cis and trans-regulatory networks were constructed based on EMT-related lncRNAs. We further estimated the infiltration of the immune cells in CAD patients with the CIBERSORT algorithm, and the correlation between key genes and infiltrating immune cells was analyzed.ResultsIn this study, a logistic regression model with powerful diagnostic capability was constructed based on a total of eight EMT-related lncRNAs identified by two machine learning methods. Then, results of the immune analysis revealed three significant immune cell subsets (CD8 T cells, monocytes, and NK cells) in CAD patients and found EMT-related lncRNAs were closely correlated with these immune cell subsets. By Pearson correlation analysis we got 34 “cis” and “trans” genes. Among them, SNAI2, an EMT-TF gene, was found in the trans-regulatory network of EMT-related lncRNAs. Further, through logistic regression and analysis of immune cell infiltration, we found SNAI2 was a potential biomarker for the diagnosis of CAD but also a close correlation between highly expressed SNAI2 and these three immune cell subsets in CAD patients.ConclusionIn conclusion, these biomarkers have important significance in the diagnosis of CAD patients. Eight EMT-related lncRNAs and SNAI2 can improve our understanding of the molecular mechanism between EMT and CAD.
Different Adaptive Strategies of Three Mangrove Species to Nutrient Enrichment: Revealed by Trait-based Analysis
Authors: Wei Lili , Hong Hualong , Bee Ming Yang , Wu Yangyang , Ndayambaje Patrick , Yan Chongling , Kao Shuh-Ji , Chee Poh Seng , Wang Yuzhe 
Published: November 2021

Abstract
Mangrove species are undergoing environmental changes from nutrient-poor to nutrient enrichment due to the large input of external nutrients. The potential difference in adaptive strategies between the slow- and fast-growing species may lead to great changes in species interaction and ecosystem functioning. This study aims to test whether the response strategies to soil nutrient availability differ between the slow- and fast-growing mangrove species. The comparison was carried out among three common mangrove species including two slow-growing species Aegiceras corniculata and Kandelia obovata, and one intrinsic fast-growing species Laguncularia racemosa. All tested species showed conservative strategies (such as slow-growing and high concentrations of leaf tolerance/resistance traits) when living in the nutrient-poor soils. But when soil nutrient increased, L. racemosa shifted to a fast-growing strategy, accompanied by a substantial reduction of tolerance traits including the concentrations of carbon, cellulose, total phenolics, and soluble sugar in leaves. In contrast, A. corniculatum and K. obovata maintained still conservative strategies even under nutrient enrichment. All the species increased leaf nitrogen, phosphorus, lipid, lignin and specific leaf area (SLA) with soil nutrient availability, but L. racemosa showed a greater nutrient acquisition capacity indicated by a steeper regression line of SLA vs. nutrient resorption efficiency than A. corniculatum. Further, the steeper regression line of SLA vs. leaf δ13C of L. racemosa indicated a higher water use efficiency than A. corniculatum. The dependence of the adaptive strategies of these species to soil nutrient status improved the standing of plant-plant interactions at different soil nutrient status.
Montmorillonite-Nasal-Gel, A Prophylactic Measure Against Covid-19
Authors: Bai Tiankai , Tian Jing , Zhai Xuan , Gao Xingyi , Wang Kangmin , Zhang Zhiyong , Yan Bin 
Published: November 2021

Abstract
Since December 2019, a novel pneumonia caused by a novel coronavirus (SARS-CoV-2) has spread through the whole world. There are various ways to control the outbreak, such as vaccines, neutralizing antibodies, but prophylactic measures is still one of the important measures to control the outbreak like face masks. Montmorillonite-Nasal-Gel (MNG), a nasal spray gel consisting of a combination of 10% montmorillonite, 2% xylitol and water, has shown the effect to reduce viral infection. We tested the effect of MNG on SARS-CoV-2 infection by using a SARS-CoV-2 S protein pseudovirus system. To evaluate the efficacy of MNG against SARS-CoV-2 infection, SARS-CoV-2 were treated with 50% MNG in BSL-3 laboratory. These experiments appeared that MNG treatment greatly reduced the infection of SARS-CoV-2. As a nasal spray, it should not stimulate the airways. So we did a respiratory stimulating test and found that it has no stimulating effect on the respiratory tract. Montmorillonite is generally considered to have a amount of negative charge on the surface, it can absorb some viruses and bacteria. We assume that montmorillonite can physically adsorb SARS-CoV-2 too. As a personal protective equipment, MNG was approved by National Medical Products Administration (NMPA). MNG can further be used in the protection of all groups.
Neutrons magnetic Mass
Authors: geilhaupt manfred 
Published: November 2021

Abstract
In Quantum Physics the Spin of an elementary particle is defined to be an „intrinsic, inherent“ property. The same to the magnetic moment (μ) due to the spin of charged particles - like Electron (me) and Proton (mp). So the intrinsic spin (S=1/2h-bar) of the electron entails a magnetic moment because of charge (e). However, a magnetic moment of a charged particle can also be generated by a circular motion (due to spin) of an electric charge (e), forming a current. Hence the „orbital motion of charge“ around a „mass-nucleus“ generates a magnetic moment by Ampère’s law. This concept leads to an alternative way calculating the neutrino mass (mν) while discussing the beta decay of a neutron into fragments: proton, electron, neutrino and binding energy. The change of neutrons magnetic moment (μn) during the decay process based on energy and spin and charge conservation should allow to calculate the restmass of the neutrino.  (KATRIN <1.1eV (2019) about 0.2eV (2021). Estimation from μn: 0.10(20)eV (2020).
Selection and Ranking of the Most Suitable Drones for Sustainable Traffic Management Using Multi-Criteria Analysis Approach
Authors: Hamurcu Mustafa , EREN Tamer 
Published: November 2021

Abstract
The traffic problem is one of the significant issues facing many large cities. So, transportation plans should be analyzed very well. Static cameras are tools and the right solution for traffic monitoring and management. But, nowadays, drones come into prominence as popular, effective, and more sustainable tools in traffic control and have been used for various traffic applications. In this study, a model is proposed for the selection of the most suitable drone under the specific characteristics to ensure a contributor to traffic management efforts. The decision model is structured with AHP and MOORA-TOPSIS and VIKOR ranking methods. The weighting of criteria is carried out by the AHP method, and a combination of AHP and ranking methods are used for the best selection. The results of the analysis were compared using Spearman's rank correlation, and it is seen that the results are at the desired level.
Integration of kinematic and dynamic mathematical models of a two-axle electric car in the problem of estimating its stability on turns
Authors: Horeniuk Vadym 
Published: November 2021

Object of research: the process of movement of cars with internal combustion engines or the electric drive on a road curve.
Investigation problem: assessment of the stability of cars with internal combustion engines or electric drive on a road curve and determination of conditions of its ensuring.
The main scientific result. The article evaluated the stability of cars with internal combustion engines or electric drive on a road curve and determines the conditions of its ensuring using an algorithm that combines mathematical models of car movement on a road curve, synthesized based on balance equations of both kinematics and dynamics. The proposed models consider the change in speed of cars while driving on a road curve, and therefore belong to the class of differential equations. The analysis of these models allows calculating changes in time of values of limiting and critical speeds of movement of the car on a road curve. The article identifies the prospects of integration into this set of mathematical models another one, synthesized in the space of linguistic variables that characterize the uncertainty of the road surface and the degree of tire wear on different wheels of the car.
The area of practical application of the research results: Automotive enterprises specializing in equipping cars with traffic control systems.
Innovative technological product: A method of determining the limiting parameters of movement of the car on road curves, at which the car does not overturn while passing turns, and an algorithm for its implementation, which combines kinematic and dynamic mathematical models of car movement on the road curve.
Scope of application of the innovative technological product: Equipping cars with additional control systems that assess the critical values of the traffic parameters on turns to ensure the conditions of non-overturning when the car passes these turns
Prognosis role of epitelial-mezenchimal transformation markers and surface stem tumor cells in the reccurence of serous low grade ovarian carcinomas
Authors: Hryhorenko Varvara 
Published: November 2021

Ovarian cancer remains one of the most fatal pathologies among women around the world due to late diagnosis on the advanced stages of the tumor process. Serous ovarian carcinomas (SOC) often recur, which worsens the prognosis for patients’ recovery and survival. The identification of prognostic clinical and morphological factors that predict the appearance of recurrence remains an urgent problem.
The aim of the research was studying relationships between the phenomenon of epithelial-mesenchymal transformation (EMT) and the expression of surface cancer stem cells (CSCS) markers to identify recurrence predictors among women with low grade serous ovarian carcinomas (LGSC).
The material were paraffin blocks and slides of 43 patients with LGSC I-IV FIGO stage. The study included 30 cancers without recurrence and 13 tumors with it within 24 months. The expression of E-cadherin, Vimentin, CD44 and CD117 was studied using immunohistochemical (IHC) method.
Results. Development of recurrence is typical for women with stage III-IV (p=0,01), the expression of Vimentin at level 51–100 % (p=0,001) and E-cadherin at 10–50 % (p=0.04). CD44 was expressed in 51.16 % of tumors and level didn`t depend on age, recurrence, but depended on disease stage (p=0.001). Recurrent LGSCs are characterized by the expression of CD117> 10 % (p=0.0001), its direct correlation with the stage (p=0.0001) and the recurrence (p=0.0001). A direct relationship was found between the CD117 and Vimentin expression.
Conclusions. Prognostic markers of recurrence should be considered stage III-IV, levels of Vimentin 51–100 %, E-cadherin 10-50 % and CD117> 10 %. A correlation between CD117 and Vimentin expression indicates the commonality of EMT and CSCS in progression and recur. CD44 has no independent prognostic role.
The role of tumoral stroma in determining the forecast of recurrence and fatal consequence of stage IIA-IIIB colorectal cancer
Authors: Yakimenko Alina 
Published: November 2021

Colorectal cancer (CRC) ranks 2nd in the structure of mortality from malignant neoplasms (MN). One of the criteria for the prognosis of CRC may be a tumor stroma, which has not been widely used in clinical practice.
The aim was to determine the main morphological features of the stroma CRC T3N0-2M0 and search for prognostic criteria for their recurrence and lethal outcome according to the operating material and autopsy.
Materials and methods. Group I included primary CRC without recurrence. The average recurrence-free period was 5 years (62.5±16.5 months). Group II – primary CRC with recurrence; IIA – with recurrences within 5 years from the date of tumor removal that did not lead to death; IIB – with the appearance of recurrence and lethal consequence of the generalization of the tumor process within 5 years from the moment of removal of the primary tumor. CRC micropreparations made according to the standard method with G + E staining were studied; immunohistochemical study was performed using monoclonal antibodies to smooth muscle actin alpha.
Results. Stromal-parenchymal ratio >50 % is a prognostic criterion for recurrence (p<0.05) and shorter recurrence-free survival (p<0.001) of patients with stage IIA-IIIB CRC. Immature stroma type CRC stage IIA-IIIB is associated with the presence of tumor budding (p<0.001), G3 differentiation (p<0.01), shorter recurrence-free survival (p<0.001); among recurrent CRCs, the immature type of stroma is associated with the lethal outcome of patients (p<0.05). Expressed levels of tumor-activated fibroblasts are one of the criteria for immature CRC stroma (p<0.003), but as an independent prognostic criterion has limited prognostic value.
Conclusions. TSR and immature type of stroma are prognostic criteria for recurrence and recurrence period of CRC pT3N0-2M0, more typical of tumors of patients with recurrence and lethal outcome with the same ratio of tumors by differentiation.
Analysis of surgical treatment of duodenal ulcers with multiple combined complications
Authors: Ivanchev Pavlo , Bilyachenko Maxim , Kurbanov Anton , Lissov Oleksii 
Published: November 2021

The aim of the research. Analysis of results and development of surgical tactics for the treatment of DU with multiple combined complications.
Materials and methods. The results of the analysis of surgical treatment of duodenal ulcers with multiple combined complications (3 and 4 combined complications) for 3 periods are presented: 1st (1983–1995) (group A) – 77 patients, 2nd (2000–2007) years (group B) – 30 patients and 3rd (2008–2020) (group C) – 46 patients.
Results. 153 patients (100 %) underwent surgery for complicated duodenal ulcer (DU), of which 130 patients (84.9 %) had a combination of three complications and 23 (15.1 %) had four complications. Bleeding complications were noted in 139 of 153 patients, accounting for 90.8 %, and ulcer perforation in 69 patients, accounting for 45.1 % of all other complications. Based on the obtained data of the analysis, there is a steady tendency to increase the proportion of organ-preserving operations (OPO) by 1.5 times (from 50.7 % to 76.2 %), reducing the number of gastrectomy (GR) by 3 times (from 14.5 % to 4.8 %) and palliative operations (PAL) 3.3 times (from 15.8 % to 4.8 %) with a relatively stable number of performed organ-saving operations (OSO): in group A – 17 (24.6 %) interventions, in group B – 4 (21.1 %), group C – 6 (14.3 %).
Conclusions. The use of modern measures of endoscopic hemostasis allowed to operate on patients in the delayed period, and their share from the second period to the third increased 2.8 times. The number of patients who underwent emergency surgery with perforation of the ulcer as one of the complications decreased in the third period compared to the second by 2.6 times, due to the widespread use of PPIs in the conservative treatment of DU. According to the results of the analysis it became known that the chosen active-individualized tactics and developed algorithms for choosing the type of surgery allowed to achieve a stable level of postoperative mortality at 8.3 %.
Extraction and reverse transcription of total rna from mouse brain-derived endothelial cells.3 infected by streptococcus Suis 2
Authors: Liu Mingcheng , Kasianenko Oksana 
Published: November 2021

Streptococcus suis 2 is an important emerging zoonotic pathogen. It mainly causes meningitis in pigs. We use SS2 to infect bEnd.3 to get stable cDNA for next research on differences in gene expression and protein expression of cytokines.
The paper presents an SS2 study for bEnd.3 infection to obtain stable cDNA for subsequent study of differences in gene expression and cytokine protein expression.
Objective: The aim of this study was to extract the total RNA from mouse brain-derived Endothelial cells (bEnd.3) infected by Streptococcus suis serotype 2 (SS2) and transcript to complementary DNA (cDNA).
Materials and methods: SS2 strain were obtained from Jilin University, China. BEnd.3 was from Henan institute of Science of Technology, China. Reverse transcription kit was from Takara company, Japan. Trizol was from Bioteke company,China. Nanodrop instrument was from Thermo company, USA. Polymerase chain reaction (PCR) instrument was from Biometra company, Germany.
We used SS2 to infect bEnd.3 at a multiplicity of infection (MOI) of 100 for 12h. Cells were harvested and Trizol method was chose to extract the total RNA of bEnd.3 infected by SS2. Nanodrop instrument was used to measure the concentration of RNA and the values of OD260/280 and OD260/230. RNA were transcripted to cDNA with reverse transcription kit by PCR instrument.
Results: trizol method used in this study was reliable and high-quality RNA were obtained. Stable cDNA were obtained by reverse transcription kit.
Conclusion: in this experiment high-quality RNA was obtained and reverse transcribed to stable cDNA for subsequent detection of related cytokines. This study provides an approximate RNA extraction method and good experimental foundation for downstream research.
Immunomorphological features of female patient’s skin with androgenetic alopecia in the treatment of platelet-rich plasma in combination with topical minoxidil 2 % lotion
Authors: Salyenkova Olena 
Published: November 2021

The aim. The research was provided to obtain immunohistochemical changes in scalp biopsies of female patients with androgenetic alopecia (AGA) I-II stages according to the Ludwig scale and to detect possible changes after usage of platelet-rich plasma (PRP) injections in combination with topical minoxidil 2 % lotion.
Materials and methods. Skin biopsies of 30 patients with AGA were examined by immunohistochemistry before and after treatment, which lasted for 3 months and included 3 PRP sessions (once per month) and topical application of minoxidil 2 % lotion twice daily.
Results. It was found that AGA is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of growth polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of Collagen IV. The use of platelet-rich plasma in combination with topical 2 % minoxidil as AGA therapy leads to the normalization of immunohistochemical parameters of the skin, which indicates the possibility of its use for long-term therapeutic effect.
Conclusions. This study supplemented the understanding of the pathogenesis of AGA and serves as the basis for improving treatment regimens for this pathology. But more research is required to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.
Transfer of Meloidogyne incognita Resistance Using Marker-assisted Selection in Sorghum
Authors: Davis Richard F. , Harris-Shultz Karen R. , Knoll Joseph E. , Wang Hongliang 
Published: November 2021

Abstract

Meloidogyne incognita is a wide-spread and damaging pathogen of many important crops in the southern United States, and most sorghum genotypes allow significant levels of reproduction by the nematode. A series of greenhouse evaluations were conducted to determine whether a quantitative trait locus (QTL) that imparts a high level of resistance to Meloidogyne incognita in sorghum can effectively be transferred into diverse sorghum genotypes using marker assisted selection. Using marker-assisted selection, the resistance QTL, QTL-Sb.RKN.3.1, from ‘Honey Drip’ sorghum was crossed into five different sorghum backgrounds that included forage, sweet, and grain sorghum until the BC1F6 generation. Repeated greenhouse experiments documented that the recurrent parent genotypes were all susceptible to M. incognita and statistically similar to each other. In contrast, the BC1F6 genotypes were all highly resistant and similar to each other and similar to the resistant standard, ‘Honey Drip’. These results suggest that this resistance QTL could be introgressed using marker assisted selection into many sorghum genotypes and confer a high level of resistance to M. incognita. Thus, this QTL and its associated markers will be useful for sorghum breeding programs to incorporate M. incognita resistance into their sorghum lines.
miR-4295 Promotes the Malignant Progression of Gastric Cancer via
Targeting PTEN
Authors: Lei Xiaoyong , Gan Runliang , Yang Xiaoyan , Yang Jing , Tang Yunlian , Xie Zhizhong , Zhang Yang 
Published: November 2021

Background:
Gastric cancer (GC), one of the common clinical malignant tumors of the
digestive system, is the fourth most commonly diagnosed cancer and the second lethal cancer
worldwide and has the characteristics of high metastasis, fatality, and recurrence rate. This research
was conducted to investigate the role and mechanism of miR-4295 in gastric cancer.

Methods:
The expression capacity of miR-4295 was determined in gastric cancer tissues and its
normal tissues by qRT-PCR. PTEN expression level was detected by western blot. SGC-7901 and
MGC-803 cell lines were cultured and transfected with miR-4295 or its inhibitor. The effects of
miR-4295 on cell proliferation, colony formation, migration, and invasion in vitro were investigated.
The mutual effect between miR-4295 and PTEN in 293T cells was explored by luciferase reporter
gene assays.

Results:
The results showed that miR-4295 expression was higher in gastric cancer tissues and cell
lines, and the miR-4295 level was significantly negatively associated with the tumor size and distal
metastasis of gastric cancer. Notably, up-regulated miR-4295 promoted cell proliferation, migration
and invasion in vitro, whereas it led to contrary effects while down-regulating miR-4295 expression.
Further mechanism studies displayed that miR-4295 could directly fasten the PTEN
3’UTR and dramatically decrease the level of PTEN in vitro.

Conclusion:
The findings revealed that miR-4295 could promote gastric cancer cell proliferation,
migration and invasion, which might be attributed to targeting PTEN. Our study suggested that
miR-4295 might be a potential therapeutic target for gastric cancer.