bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic Targeting of Histone Lysine Demethylase KDM4B Blocks the Growth of Castration-Resistant Prostate Cancer
Authors: Duan Lingling , Chen Yu-An , Liang Yanping , Chen Zhenhua , Lu Jun , Fang Yong , Cao Jiazheng , Lu Jian , Zhao Hongwei , Pong Rey-Chen , Hernandez Elizabeth , Kapur Payal , Tran Tram Anh T , Smith Tristan , Martinez Elisabeth D. , Ahn Jung-mo , Hsieh Jer-Tsong , Luo Junhang , Liu Zhi-Ping 
Published: November 2021

Abstract
Background: Accumulating evidence points to epigenetic mechanisms as essential in tumorigenesis. Treatment that targets epigenetic regulators is becoming an attractive strategy for cancer therapy. The role of epigenetic therapy in prostate cancer (PCa) remains elusive. Previously we demonstrated a correlation of levels of histone lysine demethylase KDM4B with the appearance of castration resistant prostate cancer (CRPC) and identified a small molecular inhibitor of KDM4B, B3. In this study, we aim to define the role of KDM4B in promoting PCa progression and test the efficacy of B3 using clinically relevant PCa models. Methods: KDM4B was overexpressed in LNCaP cells or knocked down (KD) in 22Rv1 cells. The specificity of B3 was determined in vitro using recombinant KDM proteins and in vivo using 22Rv1 cell lysates. The efficacy of B3 monotherapy or in combination with androgen receptor (AR) antagonist enzalutamide or the mTOR inhibitor rapamycin was tested using xenograft models in castrated mice. Comparative transcriptomic analysis was performed on KDM4B KD and B3-treated 22Rv1 cells to determine the on-target (KDM4B-dependent) and off-target (non-KDM4B-associated) effects of B3.Results: Overexpression of KDM4B in LNCaP cells enhanced its tumorigenicity whereas knockdown of KDM4B in 22Rv1 cells reduced tumor growth in castrated mice. B3 suppressed the growth of both 22Rv1 and VCaP xenografts and sensitized 22Rv1 cells to enzalutamide inhibition. B3 also inhibited 22Rv1 tumor growth synergistically with rapamycin that resulted in cell apoptosis. Mechanistically, B3 inhibited expression of AR-V7 and genes involved in epithelial-to-mesenchymal transition. DNA replication stress marker gH2A.X was upregulated by B3, which is further increased when combined with rapamycin. Based on transcriptomic and biochemical analyses, B3 inhibits both H3K9me3 and H3K27me3 demethylase activity, which is believed to underlie its anti-tumor action. Conclusions: Our studies establish KDM4B as a potent target for CRPC and B3 as a potential therapeutic agent. B3 as monotherapy or in combination with other anti-PCa therapeutics offers proof of principle for the clinical translation of epigenetic therapy targeting KDMSs for CRPC patients.
SNP-Based Genetic Structure of Tunisian Durum Wheat Landraces and Distribution of the Resistance to Septoria Tritici Blotch
Authors: Ouaja Maroua , Bahri Bochra , Ferjaoui Sahbi , Medini Maher , Sri Udupa M. , Hamza Sonia 
Published: November 2021

Abstract
Background: Septoria tritici blotch (STB) has marked durum wheat production worldwide. This fungal disease is until today a challenge for farmers, researchers and breeders all united in the aim of reducing its damage and improving wheat resistance. Tunisian durum wheat landraces were reported to be valuable genetic resources for resistance to biotic and abiotic stresses and are therefore prominently deployed in breeding programs to develop new varieties adapted to fungal diseases as STB and to climate change constraints overall.Results: A total of 366 local durum wheat accessions were assessed for resistance to two virulent Tunisian isolates of Zymoseptoria tritici Tun06 and TM220 under field conditions. Population structure analysis of the durum wheat accessions, performed with 286 polymorphic SNPs (PIC >0.3) covering the entire genome, identified three genetic subpopulations (GS1, GS2 and GS3) with 22% of admixed genotypes. Interestingly, all of the resistant genotypes were among GS2 or admixed with GS2. Conclusions: This study revealed the population structure and the genetic distribution of the resistance to Z. tritici in the Tunisian durum wheat landraces. The grouping pattern of accessions appear to be associated, to some extent, with the geographical pattern of the landraces. We suggested that GS2 accessions were mostly introduced from eastern Mediterranean populations, unlike GS1 and GS3 that originated from the west. Resistant GS2 accessions belonged to landraces Taganrog, Sbei glabre, Richi, Mekki, Badri, Jneh Khotifa and Azizi. Furthermore, we suggested that admixture contributed to transmit STB resistance from GS2 resistant landraces to initially susceptible landraces such as Mahmoudi (GS1), but also resulted in the loss of resistance in the case of GS2 suscpetible Azizi and Jneh Khotifa accessions.
Trajectory Tracking For Quadrotors: An Optimization-Based Planning Followed By Controlling Approach
Authors: Kulathunga Geesara , Devitt Dmitry , Klimchik Alexandr 
Published: November 2021

Abstract
We present an optimization-based reference trajectory tracking method for quadrotor robots for slow-speed maneuvers. The proposed method uses planning followed by the controlling paradigm. The basic concept of the proposed method is an analogy to Linear Quadratic Gaussian (LQG) in which Nonlinear Model Predictive Control (NMPC) is employed for predicting optimal control policy in each iteration. Multiple-shooting (MS) is suggested over Direct-collocation (DC) for imposing constraints when modelling the NMPC. Incremental Euclidean Distance Transformation Map (EDTM) is constructed for obtaining the closest free distances relative to the predicted trajectory; these distances are considered obstacle constraints. The reference trajectory is generated, ensuring dynamic feasibility. The objective is to minimize the error between the quadrotor’s current pose and the desired reference trajectory pose in each iteration. Finally, we evaluated the proposed method with two other approaches and showed that our proposal is better than those two in terms of reaching the goal without any collision. Additionally, we published a new dataset, which can be used for evaluating the performance of trajectory tracking algorithms.
5-HT1F Receptor Agonist Ameliorates Mechanical Allodynia In Neuropathic Pain Via Induction of Mitochondrial Biogenesis and Suppression of Neuroinflammation
Authors: Zhang Long-Qing , Zhou Ya-Qun , Li Jia-Yan , Sun Jia , Zhang Shuang , Wu Jia-Yi , Gao Shao-Jie , Tian Xue-Bi , Mei Wei 
Published: November 2021

Abstract
Background: Neuropathic pain is a devastating disease affecting millions of people worldwide. Serotonin (5-hydroxytryptamine, 5-HT) has long been involved in pain modulation. Several lines of evidence indicate that 5-HT1F receptor agonists are potent inducers of mitochondrial biogenesis. In this study, we tested the hypothesis that 5-HT1F receptor agonist ameliorates mechanical allodynia in neuropathic pain via induction of mitochondrial biogenesis and suppression of neuroinflammation. Methods: Male Sprague-Dawley rats were used to establish neuropathic pain via spared nerve injury (SNI). Paw withdrawal threshold was used to evaluate mechanical allodynia. Real time polymerase chain reaction was used to examine the mitochondrial DNA (mtDNA) copy number. Western blot and immunofluorescence were used to examine the expression of target proteins. Results: Our results showed that mitochondrial biogenesis was impaired in the spinal cord of SNI rats. Moreover, activation of PGC-1α, the master regulator of mitochondrial biogenesis, attenuated established mechanical allodynia in neuropathic pain rats. Besides, neuronal 5-HT1F receptor was significantly downregulated in the spinal cord of neuropathic pain rats. Furthermore, selective 5-HT1F receptor agonist Lasmiditan attenuated established mechanical allodynia in neuropathic pain rats. Finally, Lasmiditan treatment restored mitochondrial biogenesis and suppressed neuroinflammation in the spinal cord of SNI rats. Conclusion: These results provide the first evidence that Lasmiditan ameliorates mechanical allodynia in neuropathic pain via induction of mitochondrial biogenesis and suppression of neuroinflammation in the spinal cord. Mitochondrial biogenesis inducers may become encouraging therapeutic option for the management of neuropathic pain.
Factors Associated With Suggestive of Pulmonary Hypertension Measured by Echocardiography in Patients With A Mediastinal Tumor: A Single-Center Study
Authors: Shin Dong Geum , Kang Min-Kyung , Kim Kun Il , Yang Hodong , Han Donghoon , Choi Seonghoon , Cho Jung Rae , Lee Namho 
Published: November 2021

Abstract
Ultrasound techniques are generally not used as a primary tool in the evaluation of mediastinal tumors and cysts. This study aimed to identify factors associated with pulmonary hypertension (PH) measured by transthoracic echocardiography (TTE) in patients with a mediastinal tumor. This retrospective, observational study was performed from January 2015 to December 2020. Fifty-five patients (mean age, 62 ± 13 years; 31 [56%] women) who had a mediastinal tumor and underwent TTE were included. Patients were classified as with PH or without PH. We analyzed clinical factors and echocardiographic parameters. PH was found in 21 (38%) patients. Twenty-two patients were asymptomatic, and none had symptoms associated with PH. Forty-seven (86%) patients underwent surgery, and 23 (42%) patients were diagnosed with malignant tumors. The presence of PH was not related with malignancy. Patients with PH were older than those without PH (67 ± 10 versus [vs.] 59 ± 14 years, p = 0.017). Small left ventricular (LV) systolic dimension (29.4 ± 3.6 vs. 31.6 ± 3.6 mm, p = 0.040) and dimension (4.2 ± 0.3 vs. 4.5 ± 0.3 mm, p = 0.004) and hyperdynamic LV ejection fraction (EF, 69 ± 6 vs. 65 ± 5%, p = 0.019) were associated with PH. Among them, older age, small LV dimension, and high EF were independently associated with PH.The presence of PH had no significant effect on patients’ clinical manifestation or malignancy.
Prediction of Chlorophenols Adsorption on Activated Carbons By Representative Pores Method
Authors: de Oliveira José Carlos Alexandre , Rodrigues Paulo Ricardo Moura , de Lucena Sebastião Mardonio Pereira 
Published: November 2021

Abstract
The specification of a particular activated carbon adsorbents for removal of phenol and related derivatives, from dilute aqueous solutions, is still based on lengthy trial and error experimental tests. A predictive model of adsorption of these compounds would considerably reduce the carbon selection time and could also bring new information to support more efficient carbon synthesis. The use of molecular simulation and the methodology of representative pores, proved to be adequate for quantitative prediction of phenol adsorption. Here the methodology is being extended to chlorophenols, an important class of phenol-derived pollutants. A set of ortho and para-chlorophenol isotherms were simulated for different representative pores in order to predict carbon adsorption and determine the most significative pore size. At low concentrations (1x10-4 mol/L), the pores of 8.9 and 18.5 Å are the most effective. For concentrations above 3 x10-4 mol/L pores in the range of 27.9 Å must be present in the activated carbon. The adsorption isotherm difference between ortho and para-chlorophenol, identified experimentally, was reproduced in the simulation and its origin was investigated further. Finally, the adsorption isotherms of chlorophenols for other activated carbons were predicted with the help of the model.
Efficacy of Monocyte Distribution Width in the Early Diagnosis of Sepsis: A Diagnostic Meta-Analysis
Authors: Chen Jiahao , Guo Qiang 
Published: November 2021

Abstract
Background: Delayed diagnosis of sepsis urgently requires a fast, convenient, and inexpensive method to improve the early diagnosis of sepsis. Increasing evidence showed that monocyte distribution width (MDW) could be used as a non-invasive biomarker with high sensitivity and specificity for the early diagnosis of sepsis. However, the accuracy and reliability of its diagnosis are still controversial in different studies. Method: A meta-analysis of all available studies regarding the association between MDW and the diagnosis of sepsis was performed to systematically evaluate the diagnostic efficacy of MDW in the prediction of sepsis. Results: The estimated results of all eight studies are as follows: sensitivity, 0.84 (95% CI 0.77, 0.90); specificity, 0.68 (95% CI 0.54, 0.80); PLR, 2.7 (95% CI 1.8, 4.1); NLR, 0.23 (95% CI 0.15, 0.35); DOR is 12 (95% CI 5, 25). The corresponding overall area under the curve is 0.85 (95% CI 0.82, 0.88). Conclusion: In conclusion, this meta-analysis demonstrates that MDW has high accuracy in distinguishing patients with sepsis from healthy controls for early diagnosis of sepsis. However, large-scale prospective studies and joint diagnosis with other indicators are urgently required to confirm our findings and their utilization for routine clinical diagnosis in the future.
QoS-aware and Multi-Objective Virtual Machine Dynamic Scheduling for Big Data Centers in Clouds
Authors: Li Jirui , Zhang Rui , Zheng Yafeng 
Published: November 2021

Abstract
Efficient resource scheduling is one of the most critical issues for big datacenters in clouds to provide continuous services for users. Many existing scheduling schemes based on tasks on virtual machine (VM), pursued either load balancing or migration cost under certain response time or energy efficiency, which cannot meet the true balance of the supply and demand between users and cloud providers. The paper focuses on the following multi-objective optimization problem: how to pay little migration cost as much as possible to keep system load balancing under meeting certain quality of service (QoS) via dynamic VM scheduling between limited physical nodes in a heterogeneous cloud cluster. To make these conflicting objectives coexist, a joint optimization function is designed for an overall evaluation on the basis of a load balancing estimation method, a migration cost estimation method and a QoS estimation method. To optimize the consolidation score, an array mapping and a tree crossover model are introduced, and an improved genetic algorithm (GA) based on them is proposed. Finally, empirical results based on Eucalyptus platform demonstrate the proposed scheme outperforms exiting VM scheduling models.
Clinical Application and Drug-Use-Guidance Value of Metagenomic Next-Generation Sequencing in Central Nervous System Infection
Authors: Lin Ke , Zhang Hao-Cheng , Zhang Yi , Zhou Yang , Fu Zhang-Fan , Wang Hong-Yu , Zhao Yuan-Han , Qiu Chao , Fan Ming-Xiang , Song Jie-Yu , Ai Jing-Wen , Zhang Wen-Hong 
Published: November 2021

Abstract
Background
Timely and precise etiology diagnosis is crucial for optimized medication regimens and better prognosis in central nervous system infections (CNS infections). We aimed to analyze the impact of mNGS tests on the management of patients with CNS infections.
Methods
We conducted a single-center retrospective cohort study to analyze the value of mNGS in clinical application. Three hundred sixty-nine patients with a specific CNS infection diagnosis were enrolled, and their clinical data were collected. CDI and DDI were defined in our study to describe the intensity of drug use in different groups. We also used LOH and mRS to evaluate whether the application of mNGS could benefit CNS infection patients.
Results
mNGS reported a 91.67% sensitivity in culture-positive patients and an 88.24% specificity compared with the final diagnoses. Patients performed with the mNGS test had less drug use, both total (58.77 vs. 81.18) and daily (22.6 vs. 28.12, p <0.1, McNemar) intensity of drug use, as well as the length of hospitalization (23.14 vs. 24.29). Patients with consciousness grading 1 and 3 had a decrease in CDI (Grade1, 86.49 vs. 173.37; Grade 3, 48.18 vs. 68.21), DDI (Grade1, 1.52 vs. 2.72; Grade 3, 2.3 vs. 2.45) and LOH (Grade1, 32 vs. 40; Grade 3, 21 vs. 23) with the application of mNGS. Patients infected with bacteria in CNS had a reduced CDI, DDI, and LOH in the mNGS Group, in contrast with the TraE Group. 49% of patients altered medication plans, and 24.7% of patients reduced drug intensity four days after mNGS reports, mostly due to the reduction of drug types.
Conclusion
mNGS showed its high sensitivity and specificity characteristics. mNGS may assist clinicians with more rational medication regimens and reduce the drug intensity of patients, of which the primary way was to reduce the variety of drugs, especially for severe patients and bacterial infections. mNGS has the potential value of improving the prognosis of CNS infectious patients.
The impact of regional socioeconomic deprivation on late HIV diagnoses: a cross-sectional study in Germany
Authors: PANTKE Annemarie , KOPPE Uwe , HEIDEN Matthias AN DER , MICHALSKI Niels , HOEBEL Jens , BREMER Viviane , GUNSENHEIMER-BARTMEYER Barbara , BANNERT Norbert , HANKE Kirsten 
Published: November 2021

Abstract
Background: Late HIV diagnoses are associated with significantly poorer health outcomes and facilitate onward transmission. The proportion of HIV infections diagnosed at advanced stages has remained continuously high in Germany. This study examined the effect of regional socioeconomic deprivation on late HIV presentation.Methods: We used data from the national statutory notification of newly diagnosed HIV cases collected between 2011 and 2018 with further information on the timing of diagnosis determined by the BED-Capture-ELISA test (BED-CEIA) and diagnosing physicians. Data on regional socioeconomic deprivation were derived from the German Index of Socioeconomic Deprivation (GISD). Outcome measures were a long-standing infection based on the BED-CEIA result or an infection at the stage of AIDS. The effect of socioeconomic deprivation on the timing of diagnosis was analysed using multivariable Poisson regression models with cluster-robust error variance.Results: Overall, 66.2% (n=6,185) of the patients were diagnosed with a long-standing infection and 13.1% (n=1,536) with AIDS. The proportion of late diagnoses was higher among persons with heterosexual contact compared to men who have sex with men (MSM) (76.5% long-standing and 16.4% AIDS vs. 62.4% long-standing and 11.9% AIDS). MSM living in highly deprived countryside regions were more likely to have a long-standing infection (aPR: 1.16, 95% CI 1.05-1.28) as well as AIDS (aPR: 1.41, 95% CI 1.08-1.85) at the time of diagnosis compared to MSM in less deprived countryside regions. No differences were observed among MSM from urban areas, and no differences overall among persons with heterosexual contact.Conclusions: An effect of socioeconomic deprivation on late HIV presentation was found only in MSM from countryside regions. We suggest that efforts in promoting HIV awareness and regular HIV testing are increased for heterosexual persons irrespective of socioeconomic background, and for MSM with a focus on persons living in deprived regions in the countryside.
Compassionate and Respectful Care From Clients’ Perspectives in Wollega Zones Hospitals, Wollega, West Ethiopia
Authors: Cafo Jote Markos , Bekuma Tariku Tesfaye , Hasen Tahir , Yeyi Worku Dechasa 
Published: November 2021

Abstract
Introduction

: Compassion is a deep awareness of the suffering of another coupled with the wish and action to relieve it. Respecting the patient’s right to self-determination—that is, supporting decisions that reflect the patient’s personal beliefs, values, and interest’s problems. compassionate, respectful and caring (CRC) health workforce initiative in this plan intends to address the concern of Disrespect and Abuse for clients, including laboring mothers.
Objective

Aim of the study is to assess the provision of compassionate, respectful and caring health care services among health care providers based on client’s perspective.
Methods and materials:

Hospital-based cross-sectional study was conducted from December 1-25, 2020. A semi-structured interview administered questionnaire was used to collect data from 351 participants. Epi-Data version and Stata version 14.0 were used for data entry and data analysis, respectively. Bivariable and multivariable logistic regression model was fitted to identify the factors associated with compassionate and respectful care from clinical and non-clinical staff of the selected Hospitals. The Adjusted odds ratio with 95% confidence interval and p-value less than 0.06 were used to declare the strength and association of the factors.
Results

of health care providers were reflected by their being patient while providing care. Clients who were from the poor socio status category were 3.70 times to report getting non compassionate and respectful care from the health care professionals than the clients who are at a better position of wealth status [AOR=3.70(95%CI; 1.27,10.81)]. Similarly, clients with lower grade educational status did not receive compassionate and respectful care when compared to patients with higher educational status [AOR=0.32(95%CI;0.10, 0.99)].
Conclusion

Compassionate and respectful care provided to the patients from health care providers in the selected Hospitals of Western Oromia, Ethiopia was high. However, compassionate and respectful care or services rendered from non-clinician staff was low which suggests that immediate actions are necessary to address compassionate and respectful care at hospitals, and hospital management should adopt mitigation measures and should include non-clinician staff during training on CRC or related topics.
Predicting the growth and feed intake of Bonsmara cattle on forage based rearing systems
Authors: Brand Tertius Swanepoel , Terblanche I. , Merwe Daniel Andre Van der , Jordaan J.W. , Dreyer Olga 
Published: November 2021

Abstract
This study aimed at estimating models to predict the growth and feed intake of Bonsmara bulls and heifers in backgrounding or pasture-based production systems (Ethical clearance number A16-SCI-AGR-001). Growth and intake data were collected from the Kromme Rhee Bonsmara stud in the Stellenbosch region. The growth curves of steers and heifers were modelled using the Gompertz function. Parameter estimates of the function showed that the mature weight (A) parameter was greater (P <0.05) for bulls than heifers (ca. 878.4 vs 562.1 kg, respectively). The maturation rates (parameter B) did not differ between the sexes, while the days at maximum growth (parameter C) was higher for bulls (291.5 days) than for heifers (182.4 days). Linear functions were used to describe the average feed intakes, as well as cumulative intakes, with body weights from growing bulls and heifers from the ages 6-20 months reared on lucerne hay and protein supplement. On average, it was observed that on the lucerne hay-based diet, Bonsmara bulls and heifers consumed about 2.43% of body weight daily throughout the study period. These models can be used in precision beef rearing systems to predict the production and market weights of Bonsmara calves that are either reared on hay or pasture.