bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Cytokine Profiling Identifies Axl as a New Biomarker Candidate for Active Eosinophilic Granulomatosis With Polyangiitis
Authors: Ma Jianjuan , Dong Cong , Wei Shushan , Qiu Minzhi , Wu Penghui , Ou Changxing , Zhang Bomeng , Zhang Xueyan , Yan Jie , Zhang Qingling , Zhong Nanshan 
Published: April 2021

Background: Eosinophilic granulomatosis with polyangiitis (EGPA) prognosis is generally favorable and is treated with combined corticosteroids/immunosuppressor(s) therapy. However, disease flares increase the number of clinical visits. Therefore, discovering new serum biomarkers for early identification of active EGPA is crucial.Objective: To identify reliable serum biomarkers to measure EGPA activity.Methods: The expression of 160 proteins was compared in sera from 15 inactive and 13 active EGPA patients by antibody-based microarray. Network-based analysis identified patterns in the different groups. Differentially expressed proteins (DEPs) in active disease were identified, and the correlation between their serum levels and clinical parameters was assessed. DEPs were further analyzed for GO enrichment and KEGG pathways. Finally, DEP marker candidates were validated by ELISA and Bio-plex as well as against a second cohort of 22 inactive and 18 active EGPA patients.Results: The active group presented higher peripheral and sputum eosinophil counts, FeNO, and FEV1 (% predicted) (P &lt; 0.05). Network-based analysis showed scattered expression patterns in active subjects, but no significant bias in inactive subjects. Significant differences were observed in serum levels of 19 candidate markers, all of which were higher in active EGPA (P &lt; 0.05). KEGG analysis indicated that DEPs were mainly involved in the MAPK, PI3K-Akt, RAS and Rap1 related pathways. Nine out of 19 candidate markers were positively correlated with peripheral eosinophil counts including FGF-7, SCF, GDNF, β-NGF, IGFBP-4, Axl, PIGF, Insulin, NT-4, ErbB3, OPN and BMP-4 (r = 0.693, r = 0.692, r = 0.687, r = 0.683, r = 0.671, r = 0.606, r = 0.571, r = 0.570, r = 0.516, respectively; P &lt; 0.05), while two, CD14 and MCP-3, were negatively correlated (r = −0.644 and r = −0.515; P &lt; 0.05). The higher expression of Axl, OPN, HCC-4, GDNF, and MCP-3 in active EGPA subjects was confirmed by ELISA and Custom Multiplex Bio-plex analyses.Conclusion: The serum protein profiles were significantly different between active and inactive EGPA. The expression of the candidate proteins correlated with peripheral blood eosinophil count. Serum Axl, OPN, HCC-4, GDNF, and MCP-3 levels were consistently higher in active EGPA, independent of the assessment methods. Finally, Axl had the largest AUC, indicating that this cytokine may serve as novel biomarker for the diagnosis of active EGPA.
Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates
Authors: Busch Robert T. , Karim Farzia , Sun Yvonne , Fry H. Christopher , Liu Yuzi , Zhao Chenglong , Vasquez Erick S. 
Published: April 2021

Magnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs) as surface-enhanced Raman spectroscopy (SERS) probes. Listeria monocytogenes were used as the pathogenic bacteria and the pAb-Lis-AuMNs, 300 nm diameter, were used as probes allowing facile magnetic separation and aggregation. An optimized covalent bioconjugation procedure between the magnetic nanoshells and the polyclonal antibody was performed at pH six via a carbodiimide crosslinking reaction. Spectroscopic and morphological characterization techniques confirmed the fabrication of stable pAb-Lis-AuMNs. The resulting pAb-Lis-AuMNs acted as a SERS probe for L. monocytogenes based on the targeted capture via surface binding interactions and magnetically induced aggregation. Label-free SERS measurements were recorded for the minimum detectable amount of L. monocytogenes based on the SERS intensity at the 1388 cm−1 Raman shift. L. monocytogenes concentrations exhibited detection limits in the range of 104–107 CFU ml−1, before and after aggregation. By fitting these concentrations, the limit of detection of this method was ∼103 CFU ml−1. Using a low-intensity magnetic field of 35 G, pAb-Lis-AuMNs aggregated L. monocytogenes as demonstrated with microscopy techniques, including SEM and optical microscopy. Overall, this work presents a label-free SERS probe method comprised of a surface-modified polyclonal antibody sub-micron magnetic nanoshell structures with high sensitivity and magnetic induced separation that could lead to the fabrication of multiple single-step sensors.
Glibenclamide Advantage in Treating Edema After Intracerebral Hemorrhage (GATE-ICH): Study Protocol for a Multicenter Randomized, Controlled, Assessor-Blinded Trial
Authors: Zhao Jingjing , Yang Fang , Song Changgeng , Li Li , Yang Xiai , Wang Xiaofeng , Yu Liping , Guo Jun , Wang Kangjun , Fu Feng , Jiang Wen 
Published: April 2021

Introduction: Brain edema after acute intracerebral hemorrhage (ICH) plays a critical role in the secondary injury of ICH and may heighten the potential for a poor outcome. This trial aims to explore the efficacy of small doses of oral glibenclamide in perihematomal edema (PHE) and the prognosis of patients with ICH.Methods and Analysis: The GATE-ICH trial is a multicenter randomized, controlled, assessor-blinded trial. A total of 220 adult patients with acute primary ICH in 28 study centers in China will be randomized to the glibenclamide group (glibenclamide plus guideline-recommended ICH management) or the control group (guideline-recommended ICH management). Multivariate logistic regression will be used to analyze the relationship between the treatments and primary outcome.Study Outcomes: The primary efficacy outcome is the proportion of poor functional outcomes (modified Rankin Scale ≥3) at 90 days after enrollment. The secondary efficacy outcomes include changes in the volume of ICH and PHE between the baseline and follow-up computed tomography scans as well as the clinical scores between the baseline and follow-up assessments.Discussion: The GATE-ICH trial will assess the effects of small doses of oral glibenclamide in reducing the PHE after ICH and improving the 90-day prognosis of patients.Clinical Trial Registration:www.clinicaltrials.gov., NCT03741530. Registered on November 8, 2018.Trial Status: Protocol version: May 6, 2019, Version 5. Recruitment and follow-up of patients is currently ongoing. This trial will be end in the second quarter of 2021.
Gray Matter Sampling Differences Between Subdural Electrodes and Stereoelectroencephalography Electrodes
Authors: Tantawi Mohamed , Miao Jingya , Matias Caio , Skidmore Christopher T. , Sperling Michael R. , Sharan Ashwini D. , Wu Chengyuan 
Published: April 2021

Objective: Stereoelectroencephalography (SEEG) has seen a recent increase in popularity in North America; however, concerns regarding the spatial sampling capabilities of SEEG remain. We aimed to quantify and compare the spatial sampling of subdural electrode (SDE) and SEEG implants.Methods: Patients with drug-resistant epilepsy who underwent invasive monitoring were included in this retrospective case-control study. Ten SEEG cases were compared with ten matched SDE cases based on clinical presentation and pre-implantation hypothesis. To quantify gray matter sampling, MR and CT images were coregistered and a 2.5mm radius sphere was superimposed over the center of each electrode contact. The estimated recording volume of gray matter was defined as the cortical voxels within these spherical models. Paired t-tests were performed to compare volumes and locations of SDE and SEEG recording. A Ripley's K-function analysis was performed to quantify differences in spatial distributions.Results: The average recording volume of gray matter by each individual contact was similar between the two modalities. SEEG implants sampled an average of 20% more total gray matter, consisted of an average of 17% more electrode contacts, and had 77% more of their contacts covering gray matter within sulci. Insular coverage was only achieved with SEEG. SEEG implants generally consist of discrete areas of dense local coverage scattered across the brain; while SDE implants cover relatively contiguous areas with lower density recording.Significance: Average recording volumes per electrode contact are similar for SEEG and SDE, but SEEG may allow for greater overall volumes of recording as more electrodes can be routinely implanted. The primary difference lies in the location and distribution of gray matter than can be sampled. The selection between SEEG and SDE implantation depends on sampling needs of the invasive implant.
Case Report: Expanding the Phenotypic Spectrum of Timothy Syndrome Type 1: A Sporadic Case With a de novo CACNA1C Pathogenic Variant and Segmental Ileal Dilatation
Authors: Nugud Ahmed A. , ELkholy Nermeen Mahmoud , Omar Awad Alkarim , Qazi Abid , Tzivinikos Christos , Chencheri Nidheesh , Khan Sabina , Ba'Ath Muhammad Eyad 
Published: April 2021

Background: Long QT syndactyly syndrome (long QT syndrome type 8), also known as Timothy Syndrome (TS) was first described in 1994 with still &lt;50 case reported in the literature. The full spectrum of the syndrome is not yet known.Results: Here we report a girl who presented with new onset refractory seizures and an undiagnosed cause of intermittent abdominal distention. She also had syndactyly of her fingers and toes and was found to have prolonged QT. Upon further investigations she was found to have a de novo pathogenic variant in CACNA1C, along with Segmental Ileal Dilatation (SID), and subsequently diagnosed with Timothy syndrome.Conclusion: To our knowledge, the association of Timothy Syndrome with Segmental Ileal Dilatation, was not described before.
Feelings Related to the COVID-19 Pandemic Among Patients Treated in the Oncology Clinics (Poland)
Authors: Grajek Mateusz , Działach Eliza , Buczkowska Marta , Górski Michał , Nowara Elzbieta 
Published: April 2021

Background: The number of cancer patients is constantly growing. Both WHO and IARC report that this number may reach up to 24 million new diagnosed cases in the next two decades. The proposed treatment and especially the diagnosis can have a significant impact on an individual's approach to the disease, as well as on the patient's quality of life.Objectives: The study aimed to assess the quality of life, feelings, and fear of cancer-treating oncological patients, before and during the COVID-19 pandemic.Material and Methods: The study used the standardized WHOQOL quality of life questionnaire in a shortened version, the COVID-19 fear scale (FCV-19S), and the AIS disease acceptance scale (in terms of cancer-related sensations). The questionnaire survey was conducted among patients of cancer clinics (Poland). The study was conducted in two stages–before the COVID-19 pandemic (May 2019) and during the COVID-19 pandemic (May 2020). Data from 450 correctly completed questionnaires were analyzed statistically. The obtained data were statistically processed using the Kruskal-Wallis and Mann-Whitney U test (p = 0.05).Results: Among the surveyed patients of the cancer clinic, the quality of life during the COVID-19 pandemic decreased by 2%, compared to the period before the pandemic. The frequency of negative feelings associated with cancer increased during the COVID-19 pandemic–by 11% more men, and 4.4% of women determined the frequency of negative feelings to be 2–3 times a week. The level of fear associated with COVID-19 was moderate (57.1%), with women having a higher level of fear (12.5% higher than men).Conclusion: The development of the epidemic is very important in terms of public health. COVID-19 should be considered as one of the factors that bring about sudden changes in the mental health of the population, which may result from the dynamic development of this disease, dramatic media coverage, and own experiences. It has been shown that the sudden appearance of such a large stressor causes a decrease in patients' quality of life and an increase in negative feelings associated with chronic disease.
P. Singaram: Tamil’s First Diaspora Novelist
Authors: Murugesapandian N 
Published: April 2021

Immigration is the basis for redefining the homeland. Nostalgia for the homeland, inherent in everyone, creates a state of comparison in the diaspora. In contrast to the native country, and the country of refuge. The detection of cultural differences continues. The experiences that immigrants record in their memories are shaped as Creative works. Initially the stories of the expatriate Tamils recorded as folk songs. And then formed into stories. Substantial numbers of Tamils migrated to the South East, but no novels were writtenin the early period. Both novels Kadalukkuappaal (1959) and PuyalileoruDhoni (1972) were written by novelist P. Singaram record the lives of immigrants. The novel Kadalukkuappaal is considered to be the first diaspora novel in Tamil. Experiences narrated by P. Singaram through the myth of how human searches are spread across two different lands, Tamil Nadu and South East Asia, lead to an endless world through reading. The absurdity of never-ending human existence makes everything subject to endless debate. Both novels, Kadalukkuappaaland Puyalileoru Dhoni, by P. Singaram, have documented the lives of expatriate Tamils internationally. They are also micro-inquiries into the lives of Tamils in the Diaspora.
Number of reply comments (User Comments/Interactions)
Authors: Naab Teresa K. , Küchler Constanze 
Published: April 2021

The variable ‘number of reply comments’ is an indicator of interactivity in online discussions. The number of reply comments is a simple measure of how much response a user comment has received. It is applicable if platforms that host comment sections offer the technical possibility to users to respond directly to existing user comments. The reply comments (also called ‘sub-level comments’ or ‘child comments’) then usually appear indented below the existing user comment they refer to (also called ‘top-level comment’ or ‘parent comment’).
The measure does not provide information about the quality of the interaction between the commenters. It neither covers interactivity that occurs “outside” of comment threads below top-level comments, i.e. commenters responding in new top-level comments instead of sub-level comments.
Field of application/theoretical foundation:
Normative approaches to discourse ethics (e.g. Habermas, 1992) evaluate interactivity as a prerequisite for high-quality discourses.
Example studies:

Medium

Measure

Unit of analysis

Studies

Online; online discussions below news posts

Number of reply comments (sub-level comments) to a top-level comment

Individual user comments

Naab & Küchler (work in progress)

Info about variables
Variable name/definition: Anzahl der Antwortkommentare auf einen Nutzerkommentar
Operationalization/coding instructions: Manuell: Zählen Sie die Kind-Kommentare (Antwortkommentare, Second-Level-Kommentare), die zu einem Eltern-Kommentar (Top-Level-Kommentar) verfasst wurden und tragen die Anzahl ein.
Automatisiert: Sofern ein Datensatz alle Nutzerkommentare eines Kommentarthreads, Informationen über das Eltern- bzw. Kind-Level jedes Kommentars sowie eine Zuordnung aller Kind-Kommentare zu einem Eltern-Kommentar enthält, ist es möglich, die Anzahl der Kind-Kommentare für jeden Eltern-Kommentar per Auswertungssoftware zu aggregieren.
Level of analysis: Kommentarthread (Eltern-Kommentar + alle zugehörigen Kind-Kommentare/Antwortkommentare)

References
Naab, T.K. & Küchler, C. (work in progress). Unveröffentlichtes Codebuch aus dem DFG-Projekt „Gegenseitige Sanktionierung unter NutzerInnen von Kommentarbereichen auf Nachrichtenwebseiten und auf Facebook“. Augsburg.
Habermas, J. (1992). Faktizität und Geltung: Beiträge zur Diskurstheorie des Rechts und des demokratischen Rechtsstaates. Suhrkamp.
Quantifying Planarian Behavior as an Introduction to Object Tracking and Signal Processing
Authors: Stowell Nicole C. , Goel Tapan , Shetty Vir , Noveral Jocelyne , Collins Eva-Maria S. 
Published: April 2021

ABSTRACT
Answers to mechanistic questions about biological phenomena require fluency in a variety of molecular biology techniques and physical concepts. Here, we present an interdisciplinary approach to introducing undergraduate students to an important problem in the areas of animal behavior and neuroscience—the neuronal control of animal behavior. In this lab module, students explore planarian behavior by quantitative image and data analysis with freely available software and low-cost resources. Planarians are ∼1–2-cm-long aquatic free-living flatworms famous for their regeneration abilities. They are inexpensive and easy to maintain, handle, and perturb, and their fairly large size allows for image acquisition with a webcam, which makes this lab module accessible and scalable. Our lab module integrates basic physical concepts such as center of mass, velocity and speed, periodic signals, and time series analysis in the context of a biological system. The module is designed to attract students with diverse disciplinary backgrounds. It challenges the students to form hypotheses about behavior and equips them with a basic but broadly applicable toolkit to achieve this quantitatively. We give a detailed description of the necessary resources and show how to implement the module. We also provide suggestions for advanced exercises and possible extensions. Finally, we provide student feedback from a pilot implementation.
Potensi Environmental DNA (e-DNA) Untuk Pemantauan dan Konservasi Keanekaragaman Hayati
Authors: Marfuah Siti , Kolondam Beivy Jonathan , Tallei Trina Ekawati 
Published: April 2021

(Article History: Received January 6, 2021; Revised February 12, 2021; Accepted February 28, 2021) ABSTRAK Hilangnya spesies dan adanya spesies invasif dalam suatu habitat dapat menjadi ancaman bagi spesies asli dalam satu ekosistem. Untuk itu diperlukan teknik terkini yang mampu mendeteksi keberadaan suatu organisme. Salah satu teknik yang dapat mendeteksi organisme target di lingkungan secara cepat dan akurat yaitu environmental DNA (e-DNA).Tujuan dari ulasan artikel ini yaitu untuk mengeksplorasi kemampuan e-DNA secara ekogenomik untuk pemantauan dan konservasi keanekaragaman hayati. Ulasan artikel ini menggunakan data sekunder yang diperoleh dari berbagai database yang berbasis dalam jaringan. Hasil analisis memperlihatkan bahwa dengan menggunakan pendekatan e-DNA pemantauan dan konsevasi keanekaragaman hayati dapat dideteksi sesuai dengan taksonomi organisme dan penanda molekuler. Penanda molekuler Cytochrome c Oxidase subunit 1 (COI) mampu mendeteksi berbagai spesies baik langka dan invasif. Dengan demikian dapat disimpulkan bahwa pendekatan e-DNA dapat dijadikan sebagai metode untuk pemantauan dan konsevasi keanekaragaman hayati pada berbagai ekosistem.Kata - kata kunci: environmental DNA; keanekaragaman hayati dan konservasi; penanda molekuler ABSTRACTThe loss of species and the presence of invasive species in a habitat can be a threat to native species in an ecosystem. So we need the latest techniques that are able to detect the presence of an organism. One technique that can detect target organisms in the environment quickly and accurately is environmental DNA (e-DNA). The purpose of this review article is to explore the ecogenomic ability of e-DNA for monitoring and conservation of biodiversity. This article reviews using secondary data obtained from various network-based databases. The results of the analysis show that by using the e-DNA approach, monitoring and conservation of biological diversity can be detected according to the taxonomy of organisms and molecular markers. Cytochrome c Oxidase subunit 1 (COI) molecular markers are capable of detecting a variety of both rare and invasive species. Thus it can be concluded that the e-DNA approach can be used as a method for monitoring and conservation of biological diversity in various ecosystems.Keywords: environmental DNA; biodiversity and conservation; molecular markers
Dinamika Populasi Mikroba Tanah dan Respon Pertumbuhan Vegetatif Tanaman Jagung (Zea mays L.) Terhadap Aplikasi Amelioran Pupuk Organik
Authors: Kaharu Putri Istiqoma , Tangapo Agustina Monalisa , Mambu Susan Marlein 
Published: April 2021

(Article History: Received March 4, 2021; Revised March 31, 2021; Accepted April 7, 2021) ABSTRAKPemanfaatan kembali limbah pertanian sebagai pupuk organik memiliki prospek yang baik dalam meningkatkan produktivitas lahan melalui perbaikan sifat biologi tanah. Tujuan penelitian ini adalah untuk menganalisis efek pemberian amelioran pupuk organik dari limbah jagung (Zea mays L.) terhadap peningkatan jumlah populasi mikroba tanah dan pertumbuhan tanaman jagung. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan tiga kali ulangan: AA (Amelioran pupuk organik jagung 40 kg/ha), AB (Amelioran pupuk organik jagung 80 kg/ha), UR (Urea 40 kg/ha), dan K (tanpa amelioran atau urea). Perhitungan jumlah koloni mikroba tanah menggunakan metode hitungan cawan. Hasil penelitian menunjukkan perlakuan amelioran pupuk organik berpengaruh signifikan terhadap populasi mikroba tanah dan pertumbuhan tanaman jagung pada parameter tinggi tanaman dan jumlah daun. Perlakuan amelioran pupuk organik jagung tidak menunjukkan pengaruh terhadap diameter batang. Kata kunci: Amelioran; mikroba tanah; pupuk organik; Zea mays L. ABSTRACTThe reuse of agricultural waste as organic fertilizer has good prospects in increasing land productivity by improving soil biological properties. The aims of this study was to analyze the effect of using ameliorant organic fertilizer application from corn waste to increase the number of soil microbial populations and the growth of maize plants (Zea mays L.). The method was using CRD (Complete Random Design), which consist of 4 treatments with three repetitions: AA (Ameliorant corn organic fertilizer 40 kg/ha), AB (Ameliorant corn organic fertilizer 80 kg/ha), UR (Urea 40 kg/ha), and K (without ameliorant or urea). Calculation of the number of soil microbials colonies was using the plate count method. The results showed that the treatment of ameliorant organic fertilizer had a significant effect on soil microbials population and growth of maize on the parameters of plant height and number of leaves. The treatment of organic fertilizer ameliorant showed no effect on stem diameter.Keywords: Ameliorant; Soil microbes; Organic fertilizer; Zea mays L.
Researching a Social Justice Course in a Charter School: A Duoethnographic Conversation
Authors: Lund Darren E. , Veinotte Cheryl 
Published: April 2021

This paper uses a dialogic approach following duoethnography to report on a research study conducted in a charter school offering a locally designed social justice course. This narrative approach involves a critical dialogue between two people, each of whom pushes the other to further insights and understandings. The urban prairie school under study focused on gifted learners and was funded as a public school. Multiple methods of data collection included document and policy analysis, field observations, and open-ended interviews with administrators, teachers and students who were directly involved with the social justice program. The results and discussion focus on student engagement in schools on issues of human rights and social justice, inquiry-based approaches to the curriculum, and include implications for educational policy and practice.Keywords: alternative school programs; social justice; duoethnography; inquiry learning