bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: A Novel COL1A1 Missense Mutation Associated With Dentineogenesis Imperfecta Type I
Authors: Zeng Yuting , Pan Yuhua , Mo Jiayao , Ling Zhiting , Jiang Lifang , Xiong Fu , Yan Wenjuan 
Published: June 2021

Background: Osteogenesis imperfecta (OI) is a clinical and genetic disorder that results in bone fragility, blue sclerae and dentineogenesis imperfecta (DGI), which is mainly caused by a mutation in the COL1A1 or COL1A2 genes, which encode type I procollagen.Case Report: A missense mutation (c.1463G &gt; C) in exon 22 of the COL1A1 gene was found using whole-exome sequencing. However, the cases reported herein only exhibited a clinical DGI-I phenotype. There were no cases of bone disease or any other common abnormal symptom caused by a COL1A1 mutation. In addition, the ultrastructural analysis of the tooth affected with non-syndromic DGI-I showed that the abnormal dentine was accompanied by the disruption of odontoblast polarization, a reduced number of odontoblasts, a reduction in hardness and elasticity, and the loss of dentinal tubules, suggesting a severe developmental disorder. We also investigated the odontoblast differentiation ability using dental pulp stem cells (DPSCs) that were isolated from a patient with DGI-I and cultured. Stem cells isolated from patients with DGI-I are important to elucidate their pathogenesis and underlying mechanisms to develop regenerative therapies.Conclusion: This study can provide new insights into the phenotype-genotype association in collagen-associated diseases and improve the clinical diagnosis of OI/DGI-I.
Vitamin D and Exercise Are Major Determinants of Natural Killer Cell Activity, Which Is Age- and Gender-Specific
Authors: Oh Sooyeon , Chun Sukyung , Hwang Sena , Kim Jongseok , Cho Yuri , Lee Jooho , Kwack KyuBum , Choi Sang-Woon 
Published: June 2021

BackgroundThe coronavirus-19 disease (COVID-19) pandemic reminds us of the importance of immune function, even in immunologically normal individuals. Multiple lifestyle factors are known to influence the immune function.ObjectiveThe aim was to investigate the association between NK cell activity (NKA) and multiple factors including vitamin D, physical exercise, age, and gender.MethodsThis was a cross-sectional association study using health check-up and NKA data of 2,095 subjects collected from 2016 to 2018 in a health check-up center in the Republic of Korea. NKA was measured using the interferon-γ (IFN-γ) stimulation method. The association of NKA with 25-(OH)-vitamin D (25(OH)D) and other factors was investigated by multiple logistic regression analysis.ResultsThe average age of subjects was 48.8 ± 11.6 years (52.9% of subjects were female). Among 2,095 subjects, 1,427 had normal NKA (NKA ≥ 500 pg IFN-γ/mL), while 506 had low NKA (100 ≤ NKA &lt; 500 pg/mL), and 162 subjects had very low NKA (NKA &lt; 100 pg/mL). Compared to men with low 25(OH)D serum level (&lt; 20 ng/mL), vitamin D replete men (30–39.9 ng/mL) had significantly lower risk of very low NKA (OR: 0.358; 95% CI: 0.138, 0.929; P = 0.035). In women, both low exercise (OR: 0.529; 95% CI: 0.299, 0.939; P = 0.030) and medium to high exercise (OR: 0.522; 95% CI: 0.277, 0.981; P = 0.043) decreased the risk compared to lack of physical exercise. Interestingly, in men and women older than 60 years, physical exercise significantly decreased the risk. Older-age was associated with increased risk of very low NKA in men, but not in women.ConclusionPhysical exercise and vitamin D were associated with NKA in a gender- and age-dependent manner. Age was a major risk factor of very low NKA in men but not in women.
Cnidarian Pattern Recognition Receptor Repertoires Reflect Both Phylogeny and Life History Traits
Authors: Emery Madison A. , Dimos Bradford A. , Mydlarz Laura D. 
Published: June 2021

Pattern recognition receptors (PRRs) are evolutionarily ancient and crucial components of innate immunity, recognizing danger-associated molecular patterns (DAMPs) and activating host defenses. Basal non-bilaterian animals such as cnidarians must rely solely on innate immunity to defend themselves from pathogens. By investigating cnidarian PRR repertoires we can gain insight into the evolution of innate immunity in these basal animals. Here we utilize the increasing amount of available genomic resources within Cnidaria to survey the PRR repertoires and downstream immune pathway completeness within 15 cnidarian species spanning two major cnidarian clades, Anthozoa and Medusozoa. Overall, we find that anthozoans possess prototypical PRRs, while medusozoans appear to lack these immune proteins. Additionally, anthozoans consistently had higher numbers of PRRs across all four classes relative to medusozoans, a trend largely driven by expansions in NOD-like receptors and C-type lectins. Symbiotic, sessile, and colonial cnidarians also have expanded PRR repertoires relative to their non-symbiotic, mobile, and solitary counterparts. Interestingly, cnidarians seem to lack key components of mammalian innate immune pathways, though similar to PRR numbers, anthozoans possess more complete immune pathways than medusozoans. Together, our data indicate that anthozoans have greater immune specificity than medusozoans, which we hypothesize to be due to life history traits common within Anthozoa. Overall, this investigation reveals important insights into the evolution of innate immune proteins within these basal animals.
Acute Hypertensive Retinochoroidopathy Secondary to an Anti-cancer Drug (apatinib): The First Case Report
Authors: Yao Bangtao , Liu Gang , Wang Bei , Cao Qian 
Published: June 2021

Background: Acute hypertensive retinochoroidopathy is a rare, severe ocular disease, characterized by retinal and choroidal ischaemia. Untreated cases are associated with high mortality and poor visual outcomes. Patients subjected to treatment with the anti-neoplasic drug apatinib may trigger this disease. The purpose of this article is to describe in detail an acute hypertensive retinochoroidopathy in a young Chinese woman treated with apatinib.Case Presentation: A 40-year-old young Chinese woman presented a sudden but painless reduction of visual acuity in both eyes. She was previously diagnosed with gastric cancer and metastatic ovarian adenocarcinoma. The treatment consisted radical gastrectomy, transabdominal hysterectomy, bilateral adnexectomy, and 250 mg oral apatinib per day. After 58 days of apatinib administration, the patient immediately sought consult for a sudden decrease in vision. Her blood pressure was 208/136 mmHg and, based on the clinical manifestations, the patient was diagnosed with acute hypertensive retinochoroidopathy.Conclusions: This is the first case report of an apatinib-related acute hypertensive retinochoroidopathy diagnosed using fundal photograph, fundus fluorescein angiography, and spectral-domain optical coherence tomography simultaneously. It is crucial to develop a suitable strategy for management and prevention of this adverse event.
Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance
Authors: Sharma Amit , Gaind Rajni 
Published: June 2021

Background:Acinetobacter calcoaceticus–baumannii (ACB) complex has emerged as an important nosocomial pathogen and is associated with life-threatening infections, especially among ICU patients, including neonates. Carbapenem resistance in Acinetobacter baumannii has emerged globally and is commonly mediated by blaOXA-23. Clinically significant infections with carbapenem-resistant Acinetobacter baumannii (CRAB) are a major concern since therapeutic options are limited and associated mortality is high. Early diagnosis of both the pathogen and resistance is important to initiate the optimal therapy and prevent selection of resistance. In the current study, a loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of the ACB complex and carbapenem resistance mediated by blaOXA-23.Methodology: Universal LAMP primers were designed for the detection of significant members of the ACB complex and carbapenem resistance targeting the ITS 16S–23S rRNA and blaOXA-23 gene respectively. The optimal conditions for the LAMP assay were standardized for each primer set using standard ATCC strains. The sensitivity of the LAMP assay was assessed based on the limit of detection (LOD) using different DNA concentrations and colony counts. The specificity of LAMP was determined using the non-ACB complex and non-Acinetobacter species. The results of the LAMP assay were compared with those of polymerase chain reaction (PCR).Results: The optimal temperature for the LAMP assay was 65°C, and the detection time varied with various primers designed. Using the ITS Ab1 primer, LODs of LAMP and PCR assays were 100 pg/μl and 1 ng/μl of DNA concentration and 104 cfu/ml and 108 cfu/ml of colony count, respectively. The LAMP assay was 10- and 104-fold more sensitive than PCR using DNA concentration and colony count, respectively. The LAMP assay was found to be specific for clinically important ACB complex species.Significance of the study: The LAMP assay can be applied for early detection of significant species of the ACB complex from clinical samples and their carbapenem-resistant variants. Depending on the emerging pathogen and locally prevalent resistance genes, the LAMP assay can be modified for detection of colonization or infection by various resistant bugs.
The Impact of Preoperative Sarcopenia on Survival Prognosis in Patients Receiving Neoadjuvant Therapy for Esophageal Cancer: A Systematic Review and Meta-Analysis
Authors: Jin Sheng-bo , Tian Zi-bin , Ding Xue-li , Guo Ying-jie , Mao Tao , Yu Ya-nan , Wang Kai-xuan , Jing Xue 
Published: June 2021

BackgroundSarcopenia is a poor prognostic factor in patients with esophageal cancer (EC). It can be aggravated by neoadjuvant therapy (NAT) that improves the prognosis of patients with EC. Until now, the impact of preoperative sarcopenia on survival prognosis in patients receiving NAT for EC remains unclear.MethodsWe systematically researched relevant studies in the PubMed, EMBASE, Web of Science, the Cochrane Library databases up to March 8, 2020. Prevalence of sarcopenia before and after NAT, overall survival (OS) and disease-free survival (DFS) were collected for analysis. Finally, eleven cohort studies were included.ResultsPooled analysis indicated that preoperative sarcopenia was negatively associated with OS. (HR = 1.290; 95% CI [1.078–1.543]; P = 0.005; I2 = 0.0%) and DFS (HR = 1.554; 95% CI [1.177–2.052]; P = 0.002; I2 = 0.0%) in the patients with EC receiving NAT. The prevalence of sarcopenia increased by 15.4% following NAT (95%CI [12.9%-17.9%]). Further subgroup analysis indicated that sarcopenia diagnosed following NAT (HR = 1.359; 95% CI [1.036–1.739]; P = 0.015; I2 = 6.9%) and age &gt;65 years (HR = 1.381; 95% CI [1.090– 1.749]; P = 0.007; I2 = 0.0%) were the independent risk factors for decreased OS.ConclusionsClinicians should strengthen the screening of preoperative sarcopenia in patients of EC both receiving NAT and older than 65 years and give active nutritional support to improve the prognosis of patients.Systematic Review RegistrationInternational Platform of Registered Systematic Review and Meta-Analysis Protocols (INPLASY), identifier INPLASY202050057.
Face Masks in Young Children During the COVID-19 Pandemic: Parents' and Pediatricians' Point of View
Authors: Assathiany Rémy , Salinier Catherine , Béchet Stéphane , Dolard Claire , Kochert Fabienne , Bocquet Alain , Levy Corinne 
Published: June 2021

Background: In countries with high SARS-CoV-2 circulation, the pandemic has presented many challenges on different fronts, affecting lives and livelihoods; efforts to keep schools open are among the most important. In France, to keep schools open, wearing a face mask has been mandatory for children from age 6 years since November 2020.Objective: To evaluate the acceptability and tolerance of this measure by children as well as both parents and pediatricians.Setting: Parents registered on the website of the French Association of Ambulatory Pediatrics and pediatricians members of this association.Participants: All parents and pediatricians who agreed to take part in the survey.Results: Among the 2,954 questionnaires for the parents' survey, the reasons for wearing a mask were understood by 54.6% of parents, most of whom (84.6%) explained the reasons to their children. The parents applied this measure because it was mandatory (93.4%) even if they disagreed (63.3%). When interviewed by parents, children said they were usually embarrassed (80.9%) by the mask. The main symptoms or changes of behavior attributed to the mask according to parents were headache (49.0%), speaking difficulties (45%), change in mood (45.2%) and breathing discomfort (28.1%). Among the 663 pediatricians who responded, many agreed with mandatory mask-wearing at age 6 years (67.7%). Overall, 15% of pediatricians systematically asked about the mask tolerance during the consultation. During the medical consultation, when the parents complained about the mask (64.3%), the main drawbacks were related to fog on glasses (reported by 68.2% of pediatricians), breathing discomfort (53.1% of pediatricians), cutaneous disorders (42.4% of pediatricians) and headaches (38.2% of pediatricians).Conclusion: Despite the many inconveniences reported, children agree to wear the mask better than their parents think. Pediatricians should sufficiently take the opportunity during the consultation to further explain the reasons for wearing the mask because their pedagogical role is crucial.
Assessment of Knowledge and Practices Toward COVID-19 Prevention Among Healthcare Workers in Tigray, North Ethiopia
Authors: Gebremeskel Teferi G. , Kiros Kalayu , Gesesew Hailay A. , Ward Paul R. 
Published: June 2021

Background:The incidence rate of coronavirus disease 2019 (COVID-19) is increasing in several countries despite that public health measures are put in place. Given that COVID-19 is a newly emerging disease, there is little knowledge about the disease. The present study aims to assess knowledge, perception, and preventive practices toward COVID-19 among health workers in Tigray, North Ethiopia.Materials and Methods:A health facility-based cross-sectional study was conducted among health professionals working in public hospitals. Data were collected between April and May 2020. The researchers included 403 participants and recruited them via a simple random sampling technique. To collect data, the researchers prepared a structured questionnaire guided by the WHO survey questions. Data were entered into Epi-info 7 and exported to SPSS version 20.00 for analysis. The researchers applied descriptive and inferential statistical analyses. Tables and graphs were used to describe data, and multivariate binary logistic regression was used to determine factors affecting knowledge, perception, and practices toward COVID-19 prevention.Results:Among the participants, 79, 88, and 64.3% of them had adequate knowledge, positive perception, and good practice toward preventing COVID-19, respectively. Besides, 92% of the study participants knew that the COVID-19 virus does not have curative treatment and vaccine. The findings revealed that 55% of the respondents did not use the necessary personal protective equipment (PPE) at all times. The result showed that being female [AOR: 2.43, 95% CI (1.50–3.94)] and having a work experience of 2–5 years [AOR: 2.44, 95% CI (1.10–5.39)], news media as a source information [AOR: 7.11, 95% CI (3.07–16.49)], social media as a source information [AOR: 4.59, 95% CI (2.15–9.84)], and governmental website as a source information [AOR: 4.21, 95% CI (2.15–8.27)] were reported as protective factors; and being single [AOR: 0.15, 95% CI (0.03–0.75)] was reported as risk factor toward the prevention of COVID-19.Conclusion:Most health workers had adequate knowledge and positive attitude toward COVID-19; nevertheless, a significant proportion of health workers had poor practice toward the prevention of COVID-19, including the use of PPE. Additionally, some groups of health professional showed poor practices of implementing the public health measures, hence the call for them to improve in the prevention and control of COVID-19.
Associations of Underlying Health Conditions With Anxiety and Depression Among Outpatients: Modification Effects of Suspected COVID-19 Symptoms, Health-Related and Preventive Behaviors
Authors: Nguyen Minh H. , Pham Thu T. M. , Pham Linh V. , Phan Dung T. , Tran Tien V. , Nguyen Hoang C. , Nguyen Huu C. , Ha Tung H. , Dao Hung K. , Nguyen Phuoc B. , Trinh Manh V. , Do Thinh V. , Nguyen Hung Q. , Nguyen Thao T. P. , Nguyen Nhan P. T. , Tran Cuong Q. , Tran Khanh V. , Duong Trang T. , Nguyen Tan T. , Pham Khue M. , Nguyen Lam V. , Vo Tam T. , Do Binh N. , Dang Nga H. , Le Thuy T. , Do Ngoc T. , Nguyen Hoai T. T. , Mai Thuy T. T. , Ha Dung T. , Ngo Huong T. M. , Nguyen Kien T. , Bai Chyi-Huey , Duong Tuyen Van 
Published: June 2021

Objectives: We explored the association of underlying health conditions (UHC) with depression and anxiety, and examined the modification effects of suspected COVID-19 symptoms (S-COVID-19-S), health-related behaviors (HB), and preventive behaviors (PB).Methods: A cross-sectional study was conducted on 8,291 outpatients aged 18–85 years, in 18 hospitals and health centers across Vietnam from 14th February to May 31, 2020. We collected the data regarding participant's characteristics, UHC, HB, PB, depression, and anxiety.Results: People with UHC had higher odds of depression (OR = 2.11; p &lt; 0.001) and anxiety (OR = 2.86; p &lt; 0.001) than those without UHC. The odds of depression and anxiety were significantly higher for those with UHC and S-COVID-19-S (p &lt; 0.001); and were significantly lower for those had UHC and interacted with “unchanged/more” physical activity (p &lt; 0.001), or “unchanged/more” drinking (p &lt; 0.001 for only anxiety), or “unchanged/healthier” eating (p &lt; 0.001), and high PB score (p &lt; 0.001), as compared to those without UHC and without S-COVID-19-S, “never/stopped/less” physical activity, drinking, “less healthy” eating, and low PB score, respectively.Conclusion: S-COVID-19-S worsen psychological health in patients with UHC. Physical activity, drinking, healthier eating, and high PB score were protective factors.
Single-Crystal-to-Single-Crystal Transformation and Catalytic Properties of New Hybrid Perhalidometallates
Authors: Rayes Ali , Zárate-Roldán Stephany , Ara Irene , Moncer Manel , Dege Necmi , Gimeno M. Concepción , Ayed Brahim , Herrera Raquel P. 
Published: June 2021

Two new organic–inorganic salts of perhalidometallates with protonated organic amine cations have been synthesized and characterized by X-ray diffraction and thermal analysis. (CHBMAH2)ZnBr4·3/2H2O 1 and (CHBMAH2)ZnCl4 4 [(CHBMAH2)2+: 1,3-cyclohexanebis(methylammonium)] were obtained in single-crystal form. The crystal packing in all of the obtained compounds is governed by the formation of various non-covalent intermolecular forces between tetrahalidometallate anions and organic cations, assisted by water molecules in the hydrates. Hirshfeld surface analysis denotes that the most important contributions to the crystal packing are X···H/H···X (X: Cl, Br, I) and H···H interactions. Interestingly, the compound 1,3-cyclohexanebis(methylammonium)tetrachloridozincate (II) dihydrate, (CHBMAH2)ZnCl4·2H2O 2, undergoes thermally-triggered single-crystal-to-single-crystal (SCSC) transformation upon dehydration to produce a supramolecular solid compound, 1,3-cyclohexanebis(methylammonium) tetrachloridozincate (II), (CHBMAH2)ZnCl4 4. The SCSC transformation causes changes in the lattice parameters and a structural rearrangement. Furthermore, the catalytic properties of (CHBMAH2)ZnCl4·2H2O 2 and (CHBMAH2)CdI4·2H2O 3 have been explored in the acetalization process using various uncommon alcohols, beyond methanol or ethanol, for the first time in the literature, with outstanding results, and opening the door to the formation of alternative acetals.
1907 : naissance de la sculpture moderne ? Le renouveau de la taille directe en France
Authors: Rinuy Paul-Louis 
Published: June 2021

1907 : birth of the Modern sculpture ? The revival of the direct carving in France.
Joseph Bernard, Brancusi, Derain, Matisse and Picasso produced in 1906-1907 works in stone and in wood, using a technique which was new for them : the direct carving. These works have never be considered together in a comprehensive study. Yet they bear witness of revolutionary aesthetics and constitute an essential moment in the history of 20th C. sculpture. Excessive weight has been hitherto given to Gauguin’s sculptures and to the primitive arts as sources of the works of 1907. In fact, the beginnings of a return to direct carving appear in the evolution of sculpture in France as early as the 1880s.
À la recherche de l’œuvre d’Étienne Allegrain, paysagiste de la fin du règne de Louis XIV
Authors: Lossel-Guillien Anne 
Published: June 2021

In search of the work of Etienne Allegrain, a landscape painter at the end of Louis XIV’s reign.
Etienne Allegrain (1644-1736), a member of a dynasty of artists which seems to begin with his brothers and himself, a landscape painter in the tradition of Poussin, is still very little-known, with the exception of the pictures for the marble Trianon, and his works sometimes mistaken for those of Francisque Millet.
The discovery of archives, and particularly of his testament, permits to precise his career and to define his personality. The analysis of the classical landscapes shows how he is inspired by Poussin, more precisely by the pictures of 1647-1648. He is unlikely to have seen Italy himself. A part of his production belongs to what Roger de Piles called the “style champêtre”. In spite of this, his fidelity to Poussin’s spirit situates him at the end of a period, like Houasse and Verdier for history painting.
Le portrait d’artiste au XVIIIe siècle et la critique de son temps
Authors: Martin Sylvie 
Published: June 2021

Artist’s Portrait in the 18th C. and Contemporary Criticism.
The genre of portraiture experienced an extraordinary boost during the 18th C. As official arbiters of taste, Salon critics valued portraits of artists above all others, a position which seems to mirror positive developments in the artist’s status in Enlightenment society. Artists’ portraits of artists seem to have particularly attracted critics’ attention. Based on an analysis of a large material including the Deloynes-Duplessis collection, the author lists the qualities perceived in this new genre — vivacity, quality of brushwork, etc. Portraits of artists seem to have fulfilled those criteria which satisfied both the Académie and the new sensibility of the connoisseurs.
Les « Tombeaux », quatre aquatintes de Louis-Jean Desprez
Authors: Kozérawski Robert 
Published: June 2021

The Tombeaux, Four Aquatints by Louis-Jean Desprez.
Louis-Jean Desprez (1743-1804), Grand Prix-winner at the Académie d’architecture in 1776, is best known for his work on the illustrations for J.-C. R. de Saint-Non’s Voyage pittoresque ou Description des Royaumes de Naples et de Sicile and for his activities as set designer for Gustav Ill’s royal theater in Stockholm. The four aquatints discussed here, from a series in the Bibliothèque nationale in Paris, were produced in Rome between 1779 and 1784 and can be firmly placed within the pervasive fashion for architectural fantasies in the style of Piranesi. They nonetheless reveal certain striking characteristics — a taste for the macabre, the imprecise location of figures and architectural forms (Rome, Egypt, ...), ambiguity between life and death — which can be related to a precise model only with difficulty. These elements are also found within the literary context in the Gothic novel and in parody. The author emphasizes the various paradoxes to be found in these funereal images.