bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimalisasi penggunaan Learning Management System (LMS) dalam pembelajaran virtual untuk guru di lingkungan Lembaga Pendidikan Ma’arif NU Jember
Authors: Mahbub Moh. Arif 
Published: July 2021

[Bahasa]: Pemberlakuan pembelajaran jarak jauh (PJJ) daring (dalam jaringan) akibat krisis pandemi global Covid-19 di Indonesia memberikan tantangan tersendiri khususnya bagi para pendidik. Kesiapan kompetensi guru dalam mengelola kelas daring dan kemampuan beradaptasi dengan moda pembelajaran virtual menjadi faktor kunci keberhasilan PJJ daring tersebut. Namun, data empiris menunjukkan ketidaksiapan para guru dalam mengimplemen-tasikan PJJ daring ini. Program pengabdian kepada masyarakat ini bertujuan untuk mengoptimalkan penggunaan Edmodo dalam PJJ daring. Kegiatan ini diikuti oleh 32 peserta yang merupakan para guru yang mengajar dilingkungan LP Ma’arif NU cabang Jember. Prosedur pelaksanaan program pengabdian ini terdiri dari tiga tahap yaitu eksplorasi, elaborasi, dan konfirmasi. Hasil pengabdian menunjukkan adanya respon yang sangat positif dari para peserta pelatihan terkait integrasi Edmodo dalam PJJ daring. Selanjutnya, simpulan, keterbatasan, dan rekomendasi hasil program pengabdian didiskusikan dalam artikel ini.
Kata Kunci: Edmodo, learning management system (LMS), pembelajaran jarak jauh, pembelajaran virtual
[English]: Online learning during Covid-19 outbreak in Indonesia has seriously challenged the teachers. The teachers’ competencies in managing their online courses and the level of adaptability to virtual learning environments are both crucial factors to a successful online learning. However, empirical data demonstrate the low levels of readiness regarding the implementation of this online learning. As an attempt to resolve this problem, this community service program aims to optimize the use of Edmodo in online learning. Thirty-two teachers from Ma’arif Nahdlatul Ulama Educational Institution (LP Ma’arif NU) in Jember voluntarily participated in this program. The program was held in three phases; exploration, elaboration, and confirmation. It is found that the participants mostly have positive feelings about the integration of Edmodo into online learning. Furthermore, the conclusions, limitations, and recommendations of the program results are discussed in this article.
Keywords: Edmodo, learning management system (LMS), virtual learning, distance learning
PENERAPAN MODEL PEMBELAJARAN EDUTAINMENT BERDASARKAN QUANTUM TEACHING UNTUK MENINGKATKAN MOTIVASI DAN HASIL BELAJAR IPA SISWA KELAS IXB PADA MATERI PEWARISAN SIFAT
Authors: Rahayu Dwi Nastiti 
Published: July 2021

Penelitian ini bertujuan untuk meningkatkan motivasi dan hasil belajar IPA melalui penerapan pembelajaran edutainment berdasarkan Quantum Teaching dengan kerangka rancangan TANDUR pada Materi Pewarisan Sifat Kelas IXB di SMP Negei 10 Banjarmasin, dilaksanakan dalam dua siklus, siklus I terdiri atas dua kali pertemuan dan siklus II terdiri atas dua kali pertemuan, penelitian ini dilaksanakan dari bulan Juli sampai dengan Oktober pada semester Ganjil tahun ajaran 2014-2015. Subjek penelitian adalah siswa Kelas IXB SMP Negei 10 Banjarmasin yang berjumlah 34 siswa, terdiri dari 15 siswa perempuan dan 19 siswa laki-laki. Datal penelitian diperoleh dengan menggunakan instrumen tes akhir siklus, lembar observasi penilaian afektif dan psikomotor, lembar observasi keberhasilan tindakan, angket motivasi belajar siswa, dan catatan lapangan. Berdasarkan hasil penelitian diketahui bahwa penerapan pembelajaran edutainment berdasarkan Quantum Teaching dengan kerangka rancangan TANDUR dapat meningkatkan motivasi dan hasil belajar siswa. Peningkatan motivasi belajar siswa ditunjukkan dengan peningkatan skor rata-rata yang didapat, di mana pada siklus I menunjukkan hasil sebesar 3,46 dengan kategori cukup dan meningkat pada siklus II sebesar 3,7 dengan kategori baik. Peningkatan skor rata-rata motivasi belajar diikuti juga dengan meningkatnya ketuntasan klasikal, di mana pada siklus I sebesar 35,9% dan meningkat pada siklus II sebesar 82,1%. Peningkatan hasil belajar dapat diketahui dari ranah kognitif dengan ketuntasan belajar siswa sebesar 85% menjadi 92%. Peningkatan pada ranah afektif dapat dilihat dari persentase keberhasilannya, di mana pada siklus I sebesar 82,29% dan meningkat pada siklus II sebesar 83,33%. Peningkatan pada ranah psikomotor dapat dilihat dari persentase keberhasilannya, di mana pada siklus I sebesar 65,63% dan meningkat pada siklus II sebesar 78,12%.Kata kunci: kerangka rancangan TANDUR, motivasi belajar, hasil belajar.
KERAGAMAN JENIS VEGETASI DI KAWASAN RAWA TANPA POHON DESA BATI-BATI KABUPATEN TANAH LAUT SEBAGAI MATERI PENGAYAAN MATERI MATA KULIAH EKOLOGI LAHAN BASAH
Authors: Hardiansyah Hardiansyah , Noorhidayati Noorhidayati , Mahrudin Mahrudin 
Published: July 2021

Rawa merupakan istilah umum yang digunakan untuk semua daerah yang tergenang air baik secara musiman maupun permanen dan memiliki keragaman flora dan fauna yang khas. Rawa di Kalimantan terdapat kurang lebih sebesar 10,56 juta ha, dan di Kalimantan Selatan terdapat kurang lebih 235,677 ha. Salah satu rawa tersebut adalah rawa tanpa pohon, yang juga terdapat di Desa Bati-Batiyang didominasi oleh rumput-rumputan dan herba aquatik lainnya.Rawa tanpa pohon salah satunya di Desa Bati-Bati Kecamatan Bati-Bati Kabupaten Tanah Laut, yang data keragaman jenis vegetasi di kawasan tersebut berpotensi digunakan sebagai sumber belajar Ekologi.Tujuan penelitian ini adalah mengetahui keragaman jenis vegetasi rawa tanpa pohon di Desa Bati-Bati Kabupaten Tanah Laut, dan membuat handout pengayaan Materi Matakuliah Ekologi Lahan Basah yang valid.Metode yang digunakan adalah metode deskriptif, berupa penelitian lapangan untuk mengetahui keragaman vegetasi rawa tanpa pohon dan penelitian pengembangan untuk membuat bahan ajar berupa handout.Hasil penelitian didapatkan 12 jenis yaitu Vetiveria zizanoides (bundung), Leersia hexandra (banta), Panicum virgatum (kumpai batu), Eleocharis palustris (purun), Eleocharis acicularis (rumput purun), Eleocharis dulcis (purun tikus), Ludwegia hyssopifolia (pipisangan), Leptochlea caerulencens (parupuk), Nyampheae pubescens (batang tanding), Ipomea aquatic (kangkung), Cynodon dactylon (rumput jarum) dan Mikania scandens (kangkung pagar).dan Indeks Diversitas (H’) sebesar 1,5544 dikategorikan keanekaragaman sedang. Validitas bahan ajar handout tentang keanekaragaman vegetasi di kawasan rawa tanpa pohon Desa Bati-bati kabupaten Tanah Laut sebagai pengayaan materi mata kuliah Ekologi Lahan Basah adalah sangat valid.Kata Kunci: Keanekaragaman, Vegetasi, Rawa tanpa Pohon, Handout
КЛІТИННІ ТЕХНОЛОГІЇ ОТРИМАННЯ ТЕРАПЕВТИЧНИХ МОНОКЛОНАЛЬНИХ АНТИТІЛ
Authors: Чорний С. , Луценко Т. 
Published: July 2021

Розвиток розробки та виробництва терапевтичних моноклональних антитіл набирає значних обертів завдяки високим показникам безпечності та ефективності. В даній сфері відбувається стрімкий ріст на світовому ринку на рівні 150 млрд доларів у 2019 році, і десятки нових препаратів вже пройшли етап розробки та активно проходять клінічні дослідження. У статті проведено аналітичний огляд базових технологій отримання терапевтичних моноклональних антитіл з оцінкою переваг та недоліків. Розглядаються технології починаючи з “традиційної” гібридомної технології з високою імуногенністю отримуваних препаратів, до повністю людських моноклональних антитіл. Також у статті проаналізовано критичні етапи виробництва, що включають отримання клону-продуцента, культивування, виділення та очистку антитіл.
Ключові слова: терапевтичні моноклональні антитіла, гуманізовані та химерні антитіла, рекомбінантні білки, гібридомна технологія, фаговий дисплей, виробництво
Construction and directional evolution of anti-enrofloxacin ScFv antibody for the immunoassay of fluoroquinolones
Authors: Wang Fangyu , Li Ning , Zhang Yunshang , Sun Xuxefeng , Zhao Yali , Hu Man , Fan Jianming 
Published: July 2021

Abstract
A recombinant anti-enrofloxacin single-chain antibody (scFv) was produced for the detection of enrofloxacin. An immunized mouse phage display scFv library with a capacity of 2.35×109 CFU/mL was constructed and used for anti-enrofloxacin scFv screening. After four rounds of bio-panning, 10 positives were isolated and identified successfully. The highest positive scFv was expressed in E. coli BL21. Then, its recognition mechanisms were studied using the molecular docking method. The result showed the amino acid residues Leu121 were the key residues for the binding of ScFv to ENR. Based on the results of virtual mutation, the ScFv antibody was evolved by directional mutagenesis of contact amino acid residue Leu121 to Asn. After the expression and purification, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) based on the parental and mutant ScFv were established for enrofloxacin respectively. The IC50 value of the assay established with the ScFv mutant was 1.63 ng/mL, while the parental ScFv was 21.08 ng/mL, this result showed highly increased affinity with up to 12.9-folds improved sensitivity. The mean recovery for ENR ranged from 71.80% to 117.35% with 10.46% relative standard deviation between the intra-assay and the inter-assay. The results indicate that we have obtained a highly sensitive anti-ENR scFv by the phage library construction and directional evolution, and the scFv-based IC-ELISA is suitable for the detection of ENR residue in animal derived edible tissues and milk.
Dynamic 18F-FDopa PET Imaging for Newly-diagnosed Gliomas: Is a Semi-quantitative Model Sufficient?
Authors: ZARAGORI Timothée , Doyen Matthieu , Rech Fabien , Blonski Marie , Taillandier Luc , Imbert Laetitia , Verger Antoine 
Published: July 2021

Abstract
Purpose: Even though the semi quantitative dynamic analysis of 18F-FDOPA PET effectively and non-invasively predicts isocitrate dehydrogenase (IDH) mutations in newly-diagnosed gliomas, the underlying kinetic model of 18F-FDOPA is complex. Our current study addresses whether a semi quantitative analysis indeed captures all the clinically relevant predictive features of the more sophisticated graphical and compartmental models.Methods: Thirty-seven tumour time-activity curves from 18F-FDOPA PET dynamic acquisitions of newly-diagnosed gliomas were analysed using a semi quantitative model with (Ref SQ) or without reference region (SQ), a graphical Logan model with input function (Logan) or reference region (Ref Logan), and a two-tissue compartmental model validated for 18F-FDOPA PET imaging in gliomas (2TCM). The overall predictive performance of each model for predicting IDH mutations was assessed, by an area under the curve (AUC) comparison of multivariate analyses of all parameters included in the model.Results: SQ model with an AUC of 0.733 showed comparable performances to other models with AUCs of 0.814, 0.693, 0.786, 0.863, respectively corresponding to Ref SQ, Logan, Ref Logan and 2 TCM (p≥0.11 for the pairwise comparisons with other models). SQ time-to-peak parameter had the best diagnostic performance relative to all individual parameters with an accuracy of 75.7%.Conclusions: The SQ model circumvents the complexities of the 18F-FDOPA kinetic model and yields similar performances compared to other models most notably the compartmental model for predicting IDH mutations. This validates the application of the SQ model for the dynamic analysis of 18F-FDOPA PET images in routine clinical practice for newly-diagnosed gliomas.
Uric Acid Promotes Diabetic Retinopathy Progression through Transforming Growth Factor β2(TGF-β2) Signaling and Beneficial Effects of Fenofibrate
Authors: Zhu Dandan , Mei Zhu , fang chi 
Published: July 2021

Abstract
Purpose: To elucidate the role of TGF-β2 and Fenofibrate in DR concomitant with HUA.Methods: In vitro, human retinal endothelial cells (HRECs) were incubated with high glucose(HG), HUA and HG mixed HUA respectively. The apoptosis rate was evaluated by TUNEL assay and Capase-3 activity. TGF-β2 and VEGF was detected by Western blot and real-time PCR. The therapeutic effect of Fenofibrate was revealed by detecting the level of peroxisome proliferator activated receptor a(PPARa) through Western blot and real-time PCR.Results: The level of cellular apoptosis, TGF-β and VEGF was conducted to increase most significantly in presence of HG mixed HUA stimulated HRECs. HG mixed HUA led to PPARa suppression which resulted in activated TGF-β2 expression, and this effect could be inhibited by Fenofibrate.Conclusion: TGF-β2 might be a pivotal mediator for the progression of DR with uric acid stress and Fenofibrate could be an important pathway to ameliorate diabetic complication.
Anterior lag screw position and suboptimal reduction in lateral plain as predictors of failure in cephalomedullary nailing of intertrochanteric fractures
Authors: Garabano German , Pereira Sebastian , Rodriguez Joaquin , Pesciallo Cesar Angel , Bidolegui Fernando 
Published: July 2021

Abstract
Background
Failed osteosynthesis of the proximal femur has a great impact on morbimortality, especially in elderly patients after hip fracture. The purpose of this study was to identify the variables associated with failure in intertrochanteric fractures treated with cephalomedullary nails.
Methods
We retrospectively analyzed 251 consecutive patients who underwent surgery between January 2016 and July 2019. Age, gender, fracture stability (AO/OTA), femoral neck angle (FNA), FNA difference with contralateral hip, lag screw position and tip-apex distance (TAD), union and failure rates were analyzed. Uni and multivariate logistic regression analyses were used to identify whether these variables were related to a risk of failure (cut-out, cut-through, and/or non-union).
Results
The failure rate was 9.56%: 7 non-union (2.78%), 10 cut-out (3.98%), and 7 cut-through cases (2.78%). Female sex (p = 0.018), FNA < 125° (p = 0.0036), FNA difference 7.5° with contralateral side in lateral view (p = < 0.0001), superior (p = 0.01413) - anterior (p = < 0.0001) lag screw position and TAD > 25mm (p = 0.0167) were identified as risk factors for these complications in univariate logistic regression. Female gender (OR 12.92–95CI 1.39–1736; p 0.0196), FNA difference in lateral view (OR 1.36–95CI 1.21–1.55; p < 0.001) and anterior screw position (OR 14.01 95CI 3.15–90.99; p < 0.001 ) were confirmed as independent predictors of failure in multivariate analysis.
Conclusion
This study confirmed female sex, malreduction in the lateral plane, and anterior screw position as independent predictors of failure. Every effort should be made to achieve an accurate reduction and proper implant positioning to improve failure rates.
Observed Evidences of Frequency-Dependent Site Spectral Amplification due to Strong Structural Control of Active Reverse Faults at Different Three Localities in Japan
Authors: Thabet Mostafa 
Published: July 2021

Abstract
In the present study, observed active fault zone related site amplification is calculated based on Fourier acceleration spectrum (FAS) at three different localities in Japan. For this purpose, the FASs are calculated using 26432 earthquakes recorded at 126 K-NET and KiK-net seismic stations, which are distributed on the fault zones and upthrown and downthrown sides. This observed amplification is strongly frequency-dependent because of the presence of the near-surface low-velocity flower fault structure and the deeper fault zone. Moreover, the amplification patterns at each study area are tectonic-specific patterns. Sources inside the active fault zones could produce amplification at high frequencies at stations on both fault zone and far away from the fault zone. This is because of the impact of the near-surface fault zone. Sources outside the active fault zones could not produce significant amplification at high frequencies, whereas remarkable high amplification at low frequencies exhibits a gradual increase through stations on hanging walls, fault zones, and footwalls. Remarkably, low-frequency amplification due to sources outside the active fault zones at stations on footwalls is much higher than those observed on hanging walls. Interestingly, the peaks of the low-frequency amplification are corresponding to wavelengths that approximately equalize the width of the fault zone. Diffuse field theory inversion using earthquake horizontal-to-vertical spectral ratio (EHVSR) could successfully detect the presence of fault zone low-velocity layers. However, analyzing the fault zone related site effects using HVSR is not effective because of the strong amplification related structural control of the active fault zones on the ground motions.
Re-Emergence of Syphilis in Women of Reproductive Age and its Association with the Increase in Congenital Syphilis, The Case of Mexico During the Years 2010-2019
Authors: García-Cisneros Santa , Herrera-Ortiz Antonia , Olamendi-Portugal Maria , Sanchez-Aleman Miguel A. 
Published: July 2021

Abstract
Background: Syphilis is a sexually transmitted infection that is re-emerging in different parts of the world. This infection can be transmitted during pregnancy causing neonatal syphilis. The objective of the study was to determine the trend of syphilis, congenital syphilis, and mortality from congenital syphilis among the Mexican population between the years 2010-2019. Methods. We formed databases about the incidence of syphilis, the incidence of congenital syphilis, cases of congenital syphilis, and deaths of congenital syphilis using information from the Morbidity and Mortality Yearbooks and the Bulletin of the Mexican Ministry of Health, considering age, sex, the states of Mexico, and year. The trend was analyzed using linear regression, the increase was estimated with 95% confidence intervals, and p<0.05 was considered statistically significant. Results. The incidence of syphilis increases on average 0.336 cases/100,000, being higher among women aged 15-19 years (0.693 cases). Congenital syphilis has grown, from 62 cases in the year 2010 to 372 cases in the year 2019; the cases of congenital syphilis have relation to the increase of syphilis among women aged 20-24 years. Fifty percent of the states of Mexico without cases of congenital syphilis in 2010, but only 10% in 2018. Between the years 2010 to 2017, 62 deaths from congenital syphilis were reported. Conclusion. Congenital syphilis is increasing in Mexico, as a consequence of the reemergence of syphilis among the population of reproductive age, it is necessary to attend to syphilis in various population groups.
Comprehensive Analysis of Clinical Prognosis and Molecular Characteristics of LTB4R2 in Clear Cell Renal Cell Carcinoma
Authors: Yuan Xia , Luo Chenhui , He Yi , Wang Wei 
Published: July 2021

Abstract
BackgroundClear cell renal cell carcinoma (ccRCC) is the most common renal cancer. LTB4 receptor 2 (LTB4R2, also called BLT2), as a chemokine receptor, has been reported to be upregulated in different tumors and associated with tumor cell proliferation and invasion. However, the correlation between BLT2 expression and its prognostic value in ccRCC remain to be explored.MethodsWe used the TCGA and GEO databases to evaluate the association between BLT2 expression and clinical outcome of ccRCC. The top 300 genes that most positively correlated with BLT2 were selected for functional enrichment analysis. Correlations between BLT2 expression and tumor immune characteristics in ccRCC were evaluated based on TIMER2.0.ResultsBLT2 expression was higher in ccRCC than normal tissues. Kaplan-Meier survival analysis indicated that high BLT2 expression was significantly correlated with poor overall survival (HR = 1.75, p＜0.001) and disease-speciﬁc survival (HR = 1.60, p＝0.014) for patients with ccRCC. In addition, the findings revealed that M1 marker genes had no significant correlations with BLT2 expression in ccRCC, while moderate correlations were observed between BLT2 expression and M2 marker genes. The marker genes of Tregs and T cell exhaustion positively correlated with BLT2 expression in ccRCC (p＜0.001). ConclusionBLT2 may serve as a novel prognostic biomarker and correlate with tumor immune microenvironment shaping in ccRCC. BLT2 expression potentially contributes to the regulation of TAMs, T cell exhaustion, and Tregs activation in ccRCC, providing new approaches to drive the development of new immunotherapeutic strategies for ccRCC.
Adalimumab in Vogt-Koyanagi-Harada Disease Refractory To Conventional Therapy
Authors: Yang Shizhao , Huang Zhaohao , Liu Xiuxing , Li He , Xie Lihui , Su Wenru 
Published: July 2021

Abstract
Purpose
To evaluate the short-term effectiveness and safety of adalimumab (ADA) in patients with sight-threatening Vogt-Koyanagi-Harada (VKH) disease refractory to conventional therapy.
Methods
Medical records of VKH patients who had been treated with systemic glucocorticoids and immunosuppressants but whose condition was poorly controlled were collected and analyzed. Primary outcomes comprised of best-corrected visual acuity (BCVA), intraocular inflammation, relapses, and glucocorticoid-sparing effects. Other outcomes included central macular thickness (CMT), intraocular manifestations and adverse events (AEs).
Results
Nine refractory VKH patients with a median age of 30 (16, 43) years old were enrolled in this study and received treatment for a median of 10 (7, 11) months. Mean BCVA improved from 0.36 ± 0.26 at baseline to 0.41 ± 0.28 at final visit (P = 0.266). The anterior chamber cell grade decreased from 2 (1.75, 3) + at baseline to 0.5 (0, 1.25) + cell at final visit (P < 0.001). The vitritis grade decreased from 1 (1, 1) + cell at baseline to 0 (0, 1) + cell at final visit (P < 0.001). Patients suffered a median of 1 (0, 2) relapse during treatment. CMT remained stable from 238.50 ± 144.94 µm at baseline to 219.28 ± 77.20 µm at final visit (P = 0.553). The mean prednisone dosage decreased from 21.91 ± 18.39 mg/d to 2.73 ± 4.10 mg/d (P = 0.005). No severe AEs were found during treatment.
Conclusions
The outcomes indicated that ADA was an effective and safe option for VKH patients refractory to conventional therapy by controlling inflammation, preserving visual function and reducing the daily glucocorticoid dose.
Design, Synthesis and Biological Evaluation of HDAC and VEGFR Dual Inhibitors as a Multi-targeted Anticancer Agents
Authors: Xue Xia , Zhang Yingjie , Liao Yongxiang , Sun Deqing , Li Lina , Liu Ying , Wang Yongjie , Jiang Wen , Zhang Jian , Luan Yun , Zhao Xiaogang 
Published: July 2021

Abstract
Herein a novel series of HDAC and VEGFR dual inhibitors were designed, synthesized and biologically evaluated based on the previously reported pazopanib-based HDAC and VEGFR dual inhibitors. Most target compounds showed significant HDAC1, HDAC6 and VEGFR2 inhibition, which contributed their potent antiproliferative activities against multiple cancer cells lines and significant antiangiogenic potencies in both HUVECs tube formation assay and rat thoracic aorta rings assay. Further HDAC selectivity evaluation indicated that hydroxamic acids 5 and 9e possessed similar HDAC isoform selectivity profiles to the approved HDAC inhibitor SAHA, while hydrazide 12 presented similar HDAC isoform selectivity profiles to the clinical HDAC inhibitor MS275. The VEGFR inhibition profiles of 5, 9e and 12 were similar to that of the approved VEGFR inhibitor pazopanib. The intracellular target engagements of compounds 5 and 12 were confirmed by western blot analysis. Though the mouse liver microsome metabolic stabilities of 5, 9e and 12 were inferior to than of pazopanib, these HDAC and VEGFR dual inhibitors provided lead compounds for further structural optimization to get polypharmacological anticancer agents.