bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of poor psychiatric status and sleep quality among frontline healthcare workers during and after the COVID-19 outbreak: a longitudinal study
Authors: Zhou Yifang , Ding Hailong , Zhang Yifan , Zhang Baoyan , Guo Yingrui , Cheung Teris , Hall Brian J. , Shi Tieying , Xiang Yu-Tao , Tang Yanqing 
Published: April 2021

AbstractPoor psychiatric status and sleep quality were common among frontline healthcare workers (FHWs) during the outbreak of the 2019 novel coronavirus disease (COVID-19), but the change in these mental health outcomes overtime remained unknown. This study compared the psychiatric status and sleep quality of FHWs during and after the COVID-19 outbreak in China. FHWs who volunteered to work in Hubei province (the COVID-19 epicenter) were assessed at baseline during the COVID-19 outbreak and re-assessed when they returned to their place of origin (Liaoning province) after the COVID-19 outbreak. Participants’ psychiatric status and sleep quality were measured with the Symptom CheckList-90 (SCL-90) and the Pittsburgh Sleep Quality Index (PSQI), respectively. A total of 494 FHWs was assessed at baseline and 462 at follow-up assessments. The prevalence of poor psychiatric status was 10.5% at baseline and increased to 14.9% at the follow-up assessment (P = 0.04). The corresponding figures of poor sleep quality at baseline and follow-up assessment were 16.4% and 27.9%, respectively (P < 0.001). Multiple logistic regression analysis found that severe fatigue (p = 0.003, OR = 1.266, 95% CI = 1.081–1.483), poor sleep quality (p < 0.001, OR = 1.283, 95% CI = 1.171–1.405), and history of pre-existing psychiatric disorders (p < 0.001, OR = 5.085, 95% CI = 2.144–12.06) were independently associated with higher odds of poor psychiatric status among the FHWs. Poor psychiatric status and sleep quality were common among FHWs during the COVID-19 outbreak, and the prevalence increased following their volunteer experiences. This suggests a critical need for longer-term psychological support for this subpopulation.
The effect of eviction moratoria on the transmission of SARS-CoV-2
Authors: Nande Anjalika , Sheen Justin , Walters Emma L. , Klein Brennan , Chinazzi Matteo , Gheorghe Andrei H. , Adlam Ben , Shinnick Julianna , Tejeda Maria Florencia , Scarpino Samuel V. , Vespignani Alessandro , Greenlee Andrew J. , Schneider Daniel , Levy Michael Z. , Hill Alison L. 
Published: April 2021

AbstractMassive unemployment during the COVID-19 pandemic could result in an eviction crisis in US cities. Here we model the effect of evictions on SARS-CoV-2 epidemics, simulating viral transmission within and among households in a theoretical metropolitan area. We recreate a range of urban epidemic trajectories and project the course of the epidemic under two counterfactual scenarios, one in which a strict moratorium on evictions is in place and enforced, and another in which evictions are allowed to resume at baseline or increased rates. We find, across scenarios, that evictions lead to significant increases in infections. Applying our model to Philadelphia using locally-specific parameters shows that the increase is especially profound in models that consider realistically heterogenous cities in which both evictions and contacts occur more frequently in poorer neighborhoods. Our results provide a basis to assess eviction moratoria and show that policies to stem evictions are a warranted and important component of COVID-19 control.
Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion
Authors: Elmore Joshua R. , Dexter Gara N. , Salvachúa Davinia , Martinez-Baird Jessica , Hatmaker E. Anne , Huenemann Jay D. , Klingeman Dawn M. , Peabody George L. , Peterson Darren J. , Singer Christine , Beckham Gregg T. , Guss Adam M. 
Published: April 2021

AbstractExpanding the portfolio of products that can be made from lignin will be critical to enabling a viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield production of the tricarboxylic acid cycle-derived building block chemical, itaconic acid, from model aromatic compounds and aromatics derived from lignin. We develop a nitrogen starvation-detecting biosensor for dynamic two-stage bioproduction in which itaconic acid is produced during a non-growth associated production phase. Through the use of two distinct itaconic acid production pathways, the tuning of TCA cycle gene expression, deletion of competing pathways, and dynamic regulation, we achieve an overall maximum yield of 56% (mol/mol) and titer of 1.3 g/L from p-coumarate, and 1.4 g/L titer from monomeric aromatic compounds produced from alkali-treated lignin. This work illustrates a proof-of-principle that using dynamic metabolic control to reroute carbon after it enters central metabolism enables production of valuable chemicals from lignin at high yields by relieving the burden of constitutively expressing toxic heterologous pathways.
Biased cytochrome P450-mediated metabolism via small-molecule ligands binding P450 oxidoreductase
Authors: Jensen Simon Bo , Thodberg Sara , Parween Shaheena , Moses Matias E. , Hansen Cecilie C. , Thomsen Johannes , Sletfjerding Magnus B. , Knudsen Camilla , Del Giudice Rita , Lund Philip M. , Castaño Patricia R. , Bustamante Yanet G. , Velazquez Maria Natalia Rojas , Jørgensen Flemming Steen , Pandey Amit V. , Laursen Tomas , Møller Birger Lindberg , Hatzakis Nikos S. 
Published: April 2021

AbstractMetabolic control is mediated by the dynamic assemblies and function of multiple redox enzymes. A key element in these assemblies, the P450 oxidoreductase (POR), donates electrons and selectively activates numerous (>50 in humans and >300 in plants) cytochromes P450 (CYPs) controlling metabolism of drugs, steroids and xenobiotics in humans and natural product biosynthesis in plants. The mechanisms underlying POR-mediated CYP metabolism remain poorly understood and to date no ligand binding has been described to regulate the specificity of POR. Here, using a combination of computational modeling and functional assays, we identify ligands that dock on POR and bias its specificity towards CYP redox partners, across mammal and plant kingdom. Single molecule FRET studies reveal ligand binding to alter POR conformational sampling, which results in biased activation of metabolic cascades in whole cell assays. We propose the model of biased metabolism, a mechanism akin to biased signaling of GPCRs, where ligand binding on POR stabilizes different conformational states that are linked to distinct metabolic outcomes. Biased metabolism may allow designing pathway-specific therapeutics or personalized food suppressing undesired, disease-related, metabolic pathways.
Genetic algorithm optimized node deployment in IEEE 802.15.4 potato and wheat crop monitoring infrastructure
Authors: Pal Pankaj , Sharma Rashmi Priya , Tripathi Sachin , Kumar Chiranjeev , Ramesh Dharavath 
Published: April 2021

AbstractThis proposal investigates the effect of vegetation height and density on received signal strength between two sensor nodes communicating under IEEE 802.15.4 wireless standard. With the aim of investigating the path loss coefficient of 2.4 GHz radio signal in an IEEE 802.15.4 precision agriculture monitoring infrastructure, measurement campaigns were carried out in different growing stages of potato and wheat crops. Experimental observations indicate that initial node deployment in the wheat crop experiences network dis-connectivity due to increased signal attenuation, which is due to the growth of wheat vegetation height and density in the grain-filling and physical-maturity periods. An empirical measurement-based path loss model is formulated to identify the received signal strength in different crop growth stages. Further, a NSGA-II multi-objective evolutionary computation is performed to generate initial node deployment and is optimized over increased coverage, reduced over-coverage, and received signal strength. The results show the development of a reliable wireless sensor network infrastructure for wheat crop monitoring.
Spatial variability of saturated hydraulic conductivity and its links with other soil properties at the regional scale
Authors: Usowicz Boguslaw , Lipiec Jerzy 
Published: April 2021

AbstractSaturated hydraulic conductivity (K) is a key property for evaluating soil water movement and quality. Most studies on spatial variability of K have been performed soil at a field or smaller scale. Therefore, the aim of this work was to assess (quantify) the spatial distribution of K at the larger regional scale in south-eastern Poland and its relationship with other soil properties, including intrinsic sand, silt, and clay contents, relatively stable organic carbon, cation exchange capacity (CEC) and temporally variable water content (WC), total porosity (FI), and dry bulk density (BD) in the surface layer (0–20 cm). The spatial relationships were assessed using a semivariogram and a cross-semivariogram. The studied region (140 km2) with predominantly permeable sandy soils with low fertility and productivity is located in the south-eastern part of Poland (Podlasie region). The mean sand and organic carbon contents are 74 and 0.86 and their ranges (in %) are 45–95 and 0.002–3.75, respectively. The number of individual samples varied from 216 to 228 (for K, WC, BD, FI) to 691 for the other soil properties. The best fitting models were adjusted to the empirical semivariogram (exponential) and the cross-semivariogram (exponential, Gaussian, or linear) used to draw maps with kriging. The results showed that, among the soil properties studied, K was most variable (coefficient of variation 77.3%) and significantly (p < 0.05) positively correlated with total porosity (r = 0.300) and negatively correlated with soil bulk density (r = – 0.283). The normal or close to the normal distribution was obtained by natural logarithmic and root square transformations. The mean K was 2.597 m day−1and ranged from 0.01 up to 11.54 m day−1. The spatial autocorrelation (range) of K in the single (direct) semivariograms was 0.081° (8.1 km), while it favourably increased up to 0.149°–0.81° (14.9–81 km) in the cross-semivariograms using the OC contents, textural fractions, and CEC as auxiliary variables. The generated spatial maps allowed outlining two sub-areas with predominantly high K above 3.0 m day−1in the northern sandier (sand content > 74%) and less silty (silt content < 22%) part and, with lower K in the southern part of the study region. Generally, the spatial distribution of the K values in the study region depended on the share of individual intrinsic textural fractions. On the other hand, the ranges of the spatial relationship between K and the intrinsic and relatively stable soil properties were much larger (from ~ 15 to 81 km) than between K and the temporally variable soil properties (0.3–0.9 km). This knowledge is supportive for making decisions related to land management aimed at alteration of hydraulic conductivity to improve soil water resources and crop productivity and reduce chemical leaching.
Botulinum toxin injection changes resting state cerebellar connectivity in cervical dystonia
Authors: Hok Pavel , Hvizdošová Lenka , Otruba Pavel , Kaiserová Michaela , Trnečková Markéta , Tüdös Zbyněk , Hluštík Petr , Kaňovský Petr , Nevrlý Martin 
Published: April 2021

AbstractIn cervical dystonia, functional MRI (fMRI) evidence indicates changes in several resting state networks, which revert in part following the botulinum neurotoxin A (BoNT) therapy. Recently, the involvement of the cerebellum in dystonia has gained attention. The aim of our study was to compare connectivity between cerebellar subdivisions and the rest of the brain before and after BoNT treatment. Seventeen patients with cervical dystonia indicated for treatment with BoNT were enrolled (14 female, aged 50.2 ± 8.5 years, range 38–63 years). Clinical and fMRI examinations were carried out before and 4 weeks after BoNT injection. Clinical severity was evaluated using TWSTRS. Functional MRI data were acquired on a 1.5 T scanner during 8 min rest. Seed-based functional connectivity analysis was performed using data extracted from atlas-defined cerebellar areas in both datasets. Clinical scores demonstrated satisfactory BoNT effect. After treatment, connectivity decreased between the vermis lobule VIIIa and the left dorsal mesial frontal cortex. Positive correlations between the connectivity differences and the clinical improvement were detected for the right lobule VI, right crus II, vermis VIIIb and the right lobule IX. Our data provide evidence for modulation of cerebello-cortical connectivity resulting from successful treatment by botulinum neurotoxin.
Food-related impulsivity assessed by longitudinal laboratory tasks is reduced in patients with binge eating disorder in a randomized controlled trial
Authors: Schag Kathrin , Leehr Elisabeth J. , Meneguzzo Paolo , Martus Peter , Zipfel Stephan , Giel Katrin E. 
Published: April 2021

AbstractFood-related impulsivity, i.e. a food-related attentional bias proposed to be due to increased reward sensitivity and diminished inhibitory control, has been cross-sectionally associated with binge eating disorder. To analyze changes in food-related impulsivity, we implemented longitudinal analyses of objective laboratory tasks in a randomized controlled trial called IMPULS. Patients who attended an impulsivity-focused group intervention (IG N = 31) and control patients who did not take part in the intervention (CG N = 25) were compared before (T0) and after the intervention period (T1) and at three months follow-up (T2). Patients’ impulsive gaze behavior towards food vs. neutral stimuli was measured in two eye tracking paradigms, one addressing reward sensitivity and another addressing inhibitory control. Initial fixations of food vs. neutral stimuli were increased at T0 (IG: p = .014, CG: p = .001), but not at T1 and T2 in IG (T1: p = .178, T2: p = .203) and in CG after Bonferroni correction only at T2 (T1: p = .031, T2: p = .002). Patients from IG increased dwell time on neutral stimuli at T1 contrary to patients from CG (p = .016) and rated the presented food stimuli as less positive (e.g. pleasantness p < .001 at T1 and T2). A possible explanation for this observation is reduced reward sensitivity, which implies a short-term treatment effect. Both groups showed improvement in inhibiting eye movements towards food and neutral stimuli over time (i.e. first saccade errors overall p < .001, second saccade errors overall p < .003). This could indicate increased inhibitory control due to training effects from the study paradigm. The results suggest that food-related impulsivity represents an underlying mechanism of BED and that it is modifiable by cognitive behavioral interventions.
Background factors for chronic low back pain resistant to cognitive behavioral therapy
Authors: Shimizu Keisuke , Inage Kazuhide , Orita Sumihisa , Eguchi Yawara , Shiga Yasuhiro , Koda Masao , Aoki Yasuchika , Kotani Toshiaki , Akazawa Tsutomu , Furuya Takeo , Nakamura Junichi , Takahashi Hiroshi , Suzuki-Narita Miyako , Maki Satoshi , Hagiwara Shigeo , Inoue Masahiro , Norimoto Masaki , Kinoshita Hideyuki , Sato Takashi , Sato Masashi , Enomoto Keigo , Takaoka Hiromitsu , Mizuki Norichika , Hozumi Takashi , Tsuchiya Ryuto , Kim Geundong , Otagiri Takuma , Mukaihata Tomohito , Hishiya Takahisa , Ohtori Seiji 
Published: April 2021

AbstractThis study examined the factors that inhibit the therapeutic effects of cognitive behavioral therapy (CBT) and clarify the adaptation judgment criteria of CBT. We included patients with chronic low back pain and allocated them to the adaptation (with visual analog scale [VAS] improvement) or non-adaptation group (without VAS improvement). The patients were analyzed using various psychological tests. CBT improved depressive symptoms and catastrophic thinking; however, they were not correlated with the VAS and did not directly affect low back pain improvement. The non-adaptation group showed an unexplainable/vague sense of anxiety; an excessive focus on searching for pain; a strong intimacy desire; a strong tendency of medical dependency; and fantasy or distortion of the actual experience, especially self-image. Moreover, the patients showed a low ability to objectively express or attribute meaning to pain due to poor language skills, attention-deficit hyperactivity disorder, and emotional value judgment. Individuals with the aforementioned characteristics of pre-CBT psychological tests should select a different treatment approach given the high poor-adaption possibility. Even patients with depressive or anxious symptoms are not necessarily adaptable for CBT. Therefore, pre-CBT tests for treatment suitability are necessary. Future studies should establish a protocol for psychotherapy suitable for the non-adaptation group.
Levels of pathogen virulence and host resistance both shape the antibody response to an emerging bacterial disease
Authors: Gates Daisy E. , Staley Molly , Tardy Luc , Giraudeau Mathieu , Hill Geoffrey E. , McGraw Kevin J. , Bonneaud Camille 
Published: April 2021

AbstractQuantifying variation in the ability to fight infection among free-living hosts is challenging and often constrained to one or a few measures of immune activity. While such measures are typically taken to reflect host resistance, they can also be shaped by pathogen effects, for example, if more virulent strains trigger more robust immune responses. Here, we test the extent to which pathogen-specific antibody levels, a commonly used measure of immunocompetence, reflect variation in host resistance versus pathogen virulence, and whether these antibodies effectively clear infection. House finches (Haemorhous mexicanus) from resistant and susceptible populations were inoculated with > 50 isolates of their novel Mycoplasma gallisepticum pathogen collected over a 20-year period during which virulence increased. Serum antibody levels were higher in finches from resistant populations and increased with year of pathogen sampling. Higher antibody levels, however, did not subsequently give rise to greater reductions in pathogen load. Our results show that antibody responses can be shaped by levels of host resistance and pathogen virulence, and do not necessarily signal immune clearance ability. While the generality of this novel finding remains unclear, particularly outside of mycoplasmas, it cautions against using antibody levels as implicit proxies for immunocompetence and/or host resistance.
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil
Authors: Moitinho Mara Regina , Teixeira Daniel De Bortoli , Bicalho Elton da Silva , Panosso Alan Rodrigo , Ferraudo Antonio Sergio , Pereira Gener Tadeu , Tsai Siu Mui , Borges Beatriz Maria Ferrari , La Scala Newton 
Published: April 2021

AbstractThe spatial structure of soil CO2emission (FCO2) and soil attributes are affected by different factors in a highly complex way. In this context, this study aimed to characterize the spatial variability patterns of FCO2and soil physical, chemical, and microbiological attributes in a sugarcane field area after reform activities. The study was conducted in an Oxisol with the measurement of FCO2, soil temperature (Ts), and soil moisture (Ms) in a regular 90 × 90-m grid with 100 sampling points. Soil samples were collected at each sampling point at a depth of 0–0.20 m to determine soil physical (density, macroporosity, and microporosity), particle size (sand, silt, and clay), and chemical attributes (soil organic matter, pH, P, K, Ca, Mg, Al, H + Al, cation exchange capacity, and base saturation). Geostatistical analyses were performed to assess the spatial variability and map soil attributes. Two regions (R1 and R2) with contrasting emission values were identified after mapping FCO2. The abundance of bacterial 16S rRNA,pmoA, andnifH genes, determined by real-time quantitative PCR (qPCR), enzymatic activity (dehydrogenase, urease, cellulase, and amylase), and microbial biomass carbon were determined in R1 and R2. The mean values of FCO2(2.91 µmol m−2 s−1), Ts (22.6 °C), and Ms (16.9%) over the 28-day period were similar to those observed in studies also conducted under Oxisols in sugarcane areas and conventional soil tillage. The spatial pattern of FCO2was similar to that of macropores, air-filled pore space, silt content, soil organic matter, and soil carbon decay constant. No significant difference was observed between R1 and R2 for the copy number of bacterial 16S rRNA andnifH genes, but the results of qPCR for thepmoA gene presented differences (p < 0.01) between regions. The region R1, with the highest FCO2(2.9 to 4.2 µmol m−2 s−1), showed higher enzymatic activity of dehydrogenase (33.02 µg TPF g−1dry soil 24 h−1), urease (41.15 µg NH4–N g−1dry soil 3 h−1), amylase (73.84 µg glucose g−1dry soil 24 h−1), and microbial biomass carbon (41.35 µg C g−1soil) than R2, which had the lowest emission (1.9 to 2.7 µmol m−2 s−1). In addition, the soil C/N ratio was higher in R2 (15.43) than in R1 (12.18). The spatial pattern of FCO2in R1 and R2 may not be directly related to the total amount of the microbial community (bacterial 16S rRNA) in the soil but to the specific function that these microorganisms play regarding soil carbon degradation (pmoA).
Transport of pseudothermal photons through an anharmonic cavity
Authors: Shapiro Dmitriy S. 
Published: April 2021

AbstractUnder nonequilibrium conditions, quantum optical systems reveal unusual properties that might be distinct from those in condensed matter. The fundamental reason is that photonic eigenstates can have arbitrary occupation numbers, whereas in electronic systems these are limited by the Pauli principle. Here, we address the steady-state transport of pseudothermal photons between two waveguides connected through a cavity with Bose–Hubbard interaction between photons. One of the waveguides is subjected to a broadband incoherent pumping. We predict a continuous transition between the regimes of Lorentzian and Gaussian chaotic light emitted by the cavity. The rich variety of nonequilibrium transport regimes is revealed by the zero-frequency noise. There are three limiting cases, in which the noise-current relation is characterized by a power-law, $$S\propto J^\gamma$$

S
∝

J
γ

. The Lorentzian light corresponds to Breit-Wigner-like transmission and $$\gamma =2$$

γ
=
2

. The Gaussian regime corresponds to many-body transport with the shot noise ($$\gamma =1$$

γ
=
1

) at large currents; at low currents, however, we find an unconventional exponent $$\gamma =3/2$$

γ
=
3
/
2

indicating a nontrivial interplay between multi-photon transitions and incoherent pumping. The nonperturbative solution for photon dephasing is obtained in the framework of the Keldysh field theory and Caldeira-Leggett effective action. These findings might be relevant for experiments on photon blockade in superconducting qubits, thermal states transfer, and photon statistics probing.