bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periodic ethanol supply as a path towards unlimited lifespan ofC.elegansdauer larvae
Authors: Zhang Xingyu , Penkov Sider , Kurzchalia Teymuras V. , Zaburdeav Vasily 
Published: November 2021

AbstractThe dauer larva is a specialized stage of development optimized for survival under harsh conditions that has been used as a model for stress resistance, metabolic adaptations, and longevity. Recent findings suggest that the dauer larva ofC.elegansmay utilize external ethanol as an energy source to extend their lifespan. It was shown that while ethanol may serve as an effectively infinite source of energy, some toxic compounds accumulating as byproducts of its metabolism may lead to the damage of mitochondria and thus limit the lifespan of larvae. A minimal mathematical model was proposed to explain the connection between the lifespan of dauer larva and its ethanol metabolism. To explore theoretically if it is possible to extend even further the lifespan of dauer larvae, we incorporated two natural mechanisms describing the recovery of damaged mitochondria and elimination of toxic compounds, which were previously omitted in the model. Numerical simulations of the revised model suggest that while the ethanol concentration is constant, the lifespan still stays limited. However, if ethanol is supplied periodically, with a suitable frequency and amplitude, the dauer could survive as long as we observe the system. Analytical methods further help to explain how the feeding frequency and amplitude affect the lifespan extension. Based on comparison of the model with experimental data for fixed ethanol concentration, we propose the range of feeding protocols that could lead to even longer dauer survival and can be tested experimentally.
FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
Authors: Tanno Nobuhiro , Takemoto Kazumasa , Takada-Horisawa Yuki , Shimada Ryuki , Fujimura Sayoko , Tani Naoki , Takeda Naoki , Araki Kimi , Ishiguro Kei-ichiro 
Published: November 2021

AbstractMeiotic prophase is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase, homologous chromosomes undergo synapsis to facilitate meiotic recombination yielding crossovers. It remains largely elusive how homolog synapsis is temporally maintained and destabilized during meiotic prophase. Here we show that FBXO47 is the stabilizer of synaptonemal complex during male meiotic prophase. Disruption of FBXO47 shows severe impact on homologous chromosome synapsis and DSB repair processes, leading to male infertility. Notably, in the absence of FBXO47, although once homologous chromosomes are synapsed, the synaptonemal complex is precociously disassembled before progressing beyond pachytene. Remarkably, Fbxo47 KO spermatocytes remain in earlier stage of meiotic prophase and lack crossovers, despite apparently exhibiting diplotene-like chromosome morphology. We propose that FBXO47 functions independently of SCF E3 ligase, and plays a crucial role in preventing synaptonemal complex from premature disassembly during cell cycle progression of meiotic prophase.
Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library
Authors: Konno Hideaki , Fukunishi Yoshifumi , Shibata Kazuhiro , Itoh Masayoshi , Carninci Piero , Sugahara Yuichi , Hayashizaki Yoshihide 
Published: November 2021

We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3′ ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5′ ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libraries undergo large-scale sequencing of the 3′ ends of the inserts and clustering of the tag sequences. After clustering, the nonredundant library is constructed from the original libraries, which have redundant clones. All libraries, plates, clones, sequences, and clusters are uniquely identified, and all information is saved in the database according to this identifier. At press time, our system has been in place for the past two years; we have clustered 939,725 3′ end sequences into 127,385 groups from 227 cDNA libraries/sublibraries (seehttp://genome.gse.riken.go.jp/).[The sequence data described in this paper have been submitted to the DDBJ data library under accession nos. AV00011–AV175734, AV204013–AV382295, andBB561685–BB609425.]
Single QTL Effects, Epistasis, and Pleiotropy Account for Two-thirds of the Phenotypic F2 Variance of Growth and Obesity in DU6i x DBA/2 Mice
Authors: Brockmann Gudrun A. , Kratzsch Jürgen , Haley Chris S. , Renne Ulla , Schwerin Manfred , Karle Steffanie 
Published: November 2021

Genes influencing body weight and composition and serum concentrations of leptin, insulin, and insulin-like growth factor I (IGF-I) in nonfasting animals were mapped in an intercross of the extreme high-growth mouse line DU6i and the inbred line DBA/2. Significant loci with major effects (F > 7.07) for body weight, obesity, and muscle weight were found on chromosomes 1, 4, 5, 7, 11, 12, 13, and 17, for leptin on chromosome 14, for insulin on chromosome 4, and for IGF-I on chromosome 10 at the Igf1 gene locus itself and on chromosome 18. Significant interaction between different quantitative trait loci (QTL) positions was observed (P < 0.01). Evidence was found that loci having small direct effect on growth or obesity contribute to the obese phenotype by gene–gene interaction. The effects of QTLs, epistasis, and pleiotropy account for 64% and 63% of the phenotypic variance of body weight and fat accumulation and for over 32% of muscle weight and serum concentrations of leptin, and IGF-I in the F2 population of DU6i x DBA/2 mice.[The quantitative trait loci described in this paper have been submitted to the Mouse Genome Database.]
Comparative Sequence Analysis of the X-Inactivation Center Region in Mouse, Human, and Bovine
Authors: Chureau Corinne , Prissette Marine , Bourdet Agnès , Barbe Valérie , Cattolico Laurence , Jones Louis , Eggen André , Avner Philip , Duret Laurent 
Published: November 2021

We have sequenced to high levels of accuracy 714-kb and 233-kb regions of the mouse and bovine X-inactivation centers (Xic), respectively, centered on the Xist gene. This has provided the basis for a fully annotated comparative analysis of the mouse Xic with the 2.3-Mb orthologous region in human and has allowed a three-way species comparison of the core central region, including theXist gene. These comparisons have revealed conserved genes, both coding and noncoding, conserved CpG islands and, more surprisingly, conserved pseudogenes. The distribution of repeated elements, especially LINE repeats, in the mouse Xic region when compared to the rest of the genome does not support the hypothesis of a role for these repeat elements in the spreading of X inactivation. Interestingly, an asymmetric distribution of LINE elements on the two DNA strands was observed in the three species, not only within introns but also in intergenic regions. This feature is suggestive of important transcriptional activity within these intergenic regions. In silico prediction followed by experimental analysis has allowed four new genes, Cnbp2, Ftx, Jpx, and Ppnx, to be identified and novel, widespread, complex, and apparently noncoding transcriptional activity to be characterized in a region 5′ of Xist that was recently shown to attract histone modification early after the onset of X inactivation.[The sequence data described in this paper have been submitted to the EMBL data library under accession nos. AJ421478, AJ421479, AJ421480, andAJ421481. Online supplemental data are available athttp://pbil.univ-lyon1.fr/datasets/Xic2002/data.html andwww.genome.org.]
The Complex Repeats of Dictyostelium discoideum
Authors: Glöckner Gernot , Szafranski Karol , Winckler Thomas , Dingermann Theodor , Quail Michael A. , Cox Edward , Eichinger Ludwig , Noegel Angelika Anna , Rosenthal André 
Published: November 2021

In the course of determining the sequence of the Dictyostelium discoideum genome we have characterized in detail the quantity and nature of interspersed repetitive elements present in this species. Several of the most abundant small complex repeats and transposons (DIRS-1; TRE3-A,B; TRE5-A; skipper; Tdd-4; H3R) have been described previously. In our analysis we have identified additional elements. Thus, we can now present a complete list of complex repetitive elements in D. discoideum. All elements add up to 10% of the genome. Some of the newly described elements belong to established classes (TRE3-C, D; TRE5-B,C; DGLT-A,P; Tdd-5). However, we have also defined two new classes of DNA transposable elements (DDT and thug) that have not been described thus far. Based on the nucleotide amount, we calculated the least copy number in each family. These vary between <10 up to >200 copies. Unique sequences adjacent to the element ends and truncation points in elements gave a measure for the fragmentation of the elements. Furthermore, we describe the diversity of single elements with regard to polymorphisms and conserved structures. All elements show insertion preference into loci in which other elements of the same family reside. The analysis of the complex repeats is a valuable data resource for the ongoing assembly of whole D. discoideum chromosomes.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AF135841, AF298201, AF298202, AF298203, AF298204,AF298205, AF298206, AF298207, AF298208, AF298209, AF298210 and AF298624.]
The Complete Genome Sequence of the Lactic Acid Bacterium Lactococcus lactis ssp. lactis IL1403
Authors: Bolotin Alexander , Wincker Patrick , Mauger Stéphane , Jaillon Olivier , Malarme Karine , Weissenbach Jean , Ehrlich S. Dusko , Sorokin Alexei 
Published: November 2021

Lactococcus lactis is a nonpathogenic AT-rich gram-positive bacterium closely related to the genus Streptococcus and is the most commonly used cheese starter. It is also the best-characterized lactic acid bacterium. We sequenced the genome of the laboratory strain IL1403, using a novel two-step strategy that comprises diagnostic sequencing of the entire genome and a shotgun polishing step. The genome contains 2,365,589 base pairs and encodes 2310 proteins, including 293 protein-coding genes belonging to six prophages and 43 insertion sequence (IS) elements. Nonrandom distribution of IS elements indicates that the chromosome of the sequenced strain may be a product of recent recombination between two closely related genomes. A complete set of late competence genes is present, indicating the ability of L. lactis to undergo DNA transformation. Genomic sequence revealed new possibilities for fermentation pathways and for aerobic respiration. It also indicated a horizontal transfer of genetic information fromLactococcus to gram-negative enteric bacteria ofSalmonella-Escherichia group.[The sequence data described in this paper has been submitted to the GenBank data library under accession no. AE005176.]
High-Resolution Physical and Genetic Mapping of the Critical Region for Meckel Syndrome and Mulibrey Nanism on Chromosome 17q22–q23
Authors: Paavola Paulina , Avela Kristiina , Horelli-Kuitunen Nina , Bärlund Maarit , Kallioniemi Anne , Idänheimo Niina , Kyttälä Mira , de la Chapelle Albert , Palotie Aarno , Lehesjoki Anna-Elina , Peltonen Leena 
Published: November 2021

Previously, we assigned the genes for two autosomal recessive disorders, Meckel syndrome (MKS; MIM 249000) and Mulibrey Nanism [MUL (muscle–liver–brain–eye Nanism); MIM 253250] that are enriched in the Finnish population, to overlapping genomic regions on chromosome 17q. Now, we report the construction of a bacterial clone contig over the critical region for both disorders. Several novel CA-repeat markers were isolated from these clones, which allowed refined mapping of the MKS and MUL loci using haplotype and linkage disequilibrium analysis. The localization of the MKS locus was narrowed to <1 cM between markers D17S1290 and 132-CA, within an ∼800-kb region. The MUL locus was refined into an ∼1400-kb interval between markers D17S1290 and 52-CA. The whole MKS region falls within the MUL region. In the common critical region, the conserved haplotypes were different in MKS and MUL patients. A trancript map was constructed by assigning expressed sequence tags (ESTs) and genes, derived from the human gene map, to the bacterial clone contig. Altogether, four genes and a total of 20 ESTs were precisely localized. These data provide the molecular tools for the final identification of the MKS and the MUL genes.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. G42608–G42611,G42376–G42388, and G42200–G42250. The online supplement for primer sequences and PCR product sizes, as well as the STS-content table, are available at http://www.cshl.org/gr.]
Insurance claims and audit quality: evidence from trade credit insurance in Chinese listed firms
Authors: Chen Songsheng , Guo Jun , Tian Yingying , Yan Lijuan 
Published: November 2021

PurposeUsing unique trade credit insurance data from China, we examine whether trade insurance claims are associated with audit efforts and audit quality.Design/methodology/approachThe paper is based on a sample of Chinese firms to study insurance claims of trade credit insurance that affects abnormal audit fees.FindingsIn this study, we find that firms with high insurance claims pay higher abnormal audit fees. Further, our findings indicate that firms with high insurance claims have a short audit report lag and tend to select local audit firms.Originality/valueTo the best of our knowledge, this is the first study to investigate the association between trade credit insurance claims and audit efforts. In addition, we contribute to the literature on the agency cost of abnormal audit fees.
Exploring the antecedents of industry globalization: a longitudinal investigation
Authors: Sambharya Rakesh B. , Rasheed Abdul A. , Contractor Farok J. 
Published: November 2021

PurposeThere is considerable variation in the extent of globalization across industries. The authors attempt to identify the structural conditions of the industry that lead to these variations.Design/methodology/approachBased on a sample of 33 manufacturing industries over the nine-year period from 2007 to 2016, the authors test for antecedents of industry globalization.FindingsThe authors find that industry globalization is positively affected by medium levels of barriers to entry, industry competition, industry assistance, low and mediums levels of capital intensity, industry concentration and industry regulation and negatively affected by low levels of technological change and industry assistance. In addition, the life cycle stage of the industry has an impact on the level of globalization with the growth stage having the highest level of globalization.Research limitations/implicationsFirst, the major limitation of the paper is that the authors rely entirely on trade data to measure the level of industry globalization. The authors did not have a choice because foreign direct investment (FDI) data are available only at the country level. Second, given that globalization can occur at the country, industry and firm levels, the focus on industry-level structural characteristics alone may be seen as a limitation.Practical implicationsThe results of the study can provide guidance to practicing managers to apply industry analysis for predicting the potential for and direction of globalization of their industries. This will enable them to formulate appropriate strategies to cope with global competition.Social implicationsThe study has important public policy implications. National governments have many levers at their command that can be used to influence the structural characteristics of industries, such as industry regulation, industry assistance and industry concentration. They can selectively use these levers to either facilitate or impede globalization.Originality/valueMuch of the empirical focus of prior research on globalization has been on countries, rather than industries, as the unit of analysis. There is clearly variation in the extent of globalization across industries with some industries highly integrated while others remain primarily local or regional. Based on a novel approach to measure the extent of globalization at the industry level, the authors identify its antecedents. The value of the paper lies in the fact that the analysis of 33 manufacturing industries over a ten-year period shows that the structural characteristics of the industries drive their extent of globalization.
COVID-19 black swan and personal protective equipment (PPE) in Pakistan
Authors: Ahmad Akhlaq , Shoaib Muhammad , Abdullah Farooq 
Published: November 2021

PurposeThis paper aimed to examine the sources of information and use of personal protective equipment (PPE) during the COVID-19 pandemic in the province of Punjab, Pakistan. The COVID-19 pandemic affected at a large level of all social institutions including the healthcare system of the countries.Design/methodology/approachThe study was conducted in Pakistan by using an online survey technique. The rationale to opt for this method was based on the country’s lockdown situations, social distancing and the care of the respondents. A total of 847 individuals from different parts of the Punjab province participated in the study. An attitudinal scale was administered, which consisted of statements to measure (dis)agreement among the individuals on current situations of the COVID-19 pandemic. The ethical considerations and confidentiality of the respondents were opted by describing the purpose of research on the first page of the questionnaire.FindingsThe study findings revealed that peer group, signs and symptoms of COVID-19 pandemic and causes and consequences of COVID-19 pandemic had favourable positive effects on the use of PPE by individuals through the mediation of fear of infection during COVID-19 pandemic. Furthermore, the study findings were interpreted as per the effects of current situations of COVID-19 on the sources of information and the use of PPE by individuals.Originality/valueThis study focused on an important gap in the research on COVID-19 pandemic in the country in terms of mass media, health practitioners, peer group, fear of infection and use of PPE.
Conflict resolution under the COVID-19 pandemic
Authors: Balmori de la Miyar Jose Roberto , Hoehn-Velasco Lauren , Silverio-Murillo Adan 
Published: November 2021

Purpose
This paper aims to analyze conflict resolution strategies among individuals who encountered disputes with family, neighbors, workmates, businesses, strangers, government authorities or other members in their communities during the COVID-19 pandemic.

Design/methodology/approach
Applying a difference-in-difference approach by sex, the authors use a representative panel of adults living in 82 cities from across Mexico to measure changes in antisocial strategies (violence) and prosocial strategies (non-confrontational methods and dialogue) to resolve disputes.

Findings
During the COVID-19 pandemic, men reduced their use of violence by 19.6% while women did so by 17.4%. There was a parallel rise in non-confrontational strategies used to solve conflicts equal to 73.6% for men and 62% for women. The use of dialogue as a tool for resolving disputes increased by more in cities that banned the sale of alcohol. Alternative Twitter data corroborates the main findings, suggesting that individuals are becoming more prosocial during the pandemic.

Originality/value
To the knowledge, this is the first empirical study to analyze changes in strategies for conflict resolution as a consequence of the COVID-19 pandemic. This paper contributes to the literature by demonstrating how individuals adapt their dispute strategies under extraordinary circumstances such as a pandemic, with a focus on a middle-income setting.
Development of green synthesized nanomaterials for hybrid vehicle applications
Authors: Jadhav Dayanand Bhaurao , Kokate Rajendra D. 
Published: November 2021

PurposeRenewable energy alternatives and nanoscale materials have gained huge attention in recent years due to the problems associated with fossil fuels. The recyclable battery is one of the recent developments to address the energy requirement issues. In this work, the development of nanoscale materials is focused on using green synthesis methods to address the energy requirements of hybrid electric vehicles.Design/methodology/approachThe current research focuses on developing metal oxide nanoscale materials (NANO-SMs). The Zno-Aloe vera NANO-SM is prepared using the green synthesis method. The developed nanoscale materials are characterized using analysis methods like FESEM, TEM, XRD and FTIR.FindingsThe average size of ZnO-Aloe vera mono-crystalline was recorded as 60–70 nm/Hexagonal shape. The nanoscale materials are used for the detection of LPG gases. The sensitivity observed was 48%. The response time and recovery time were recorded as 8–10 s and 230–250 s, respectively. The average size of SnO2-green papaya leaves poly-crystalline was recorded as 10–20 nm/powder form.Originality/valueNanoscale materials are developed using green synthesis methods for hybrid vehicle applications. The nanoscale materials are used for the detection of harmful gases in hybrid vehicles.
Identifying the factors of internal green marketing: a scale development and psychometric evaluation approach
Authors: Qureshi Ishtiaq Hussain , Mehraj Danish 
Published: November 2021

PurposeThe purpose of this paper is to perform a psychometric evaluation of the internal green marketing (IGM) scale in the context of consumer, industrial and service industry from a developing economy. Drawing on IGM theory, this study validates the measurement scales to operationalize IGM as green internal communication (GIC), green skill development (GSD) and green rewards (GRs).Design/methodology/approachThe sample was drawn from the consumer, industrial and service industries in Jammu and Kashmir, India, using a random sampling method. Data were collected from consumer, industrial and service industries in two phases through a self-administrated questionnaire-based survey. In total, 137 managers responded during the first phase, and 368 managers responded during the second phase. During the first phase, exploratory factor analysis (EFA) was conducted to uncover the underlying dimensions of IGM, and during the second phase, data were analyzed to test the validity of the IGM scale through confirmatory factor analysis (CFA).FindingsEFA suggested a three-dimension scale (green internal communication (GIC), green skill development (GSD), and green rewards (GR)) which was confirmed by CFA. The findings of the study demonstrate that IGM is a valid and reliable scale to capture the individual-level perception of the employees with respect to the green internal marketing of an organization.Practical implicationsThis paper is expected to provide valuable insights into the area of internal green marketing (IGM) about an industry that can be of immense help to domestic and international marketers in formulating human resource (HR)/marketing strategies. Further, it provides a wide-ranging scale that can act as a base for future research studies that aim to explore internal green marketing (IGM) in different organizational settings.Originality/valueIGM is embedded in three underlying elements as GIC, GSD and GRs. It is suitable to the practitioner and researchers to operationalize IGM as a second-order construct in future studies.