bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocadherin 7–Associated Membranous Nephropathy
Authors: Sethi Sanjeev , Madden Benjamin , Debiec Hanna , Morelle Johann , Charlesworth M. Cristine , Gross LouAnn , Negron Vivian , Buob David , Chaudhry Sidharth , Jadoul Michel , Fervenza Fernando C. , Ronco Pierre 
Published: April 2021

Significance Statement
Membranous nephropathy (MN) results from antibodies targeting an antigen in the glomerular basement membrane (GBM). The target antigens identified so far include PLA2R, THSD7A, NELL1, SEMA3B, and EXT1/EXT2. Using laser microdissection and mass spectrometry analysis, the authors identified a novel protein, protocadherin 7 (PCDH7), that is present in the GBM of a subset of patients with MN who are negative for all of the known antigens associated with MN. PCDH7 shows granular GBM staining and colocalizes with Ig in the GBM. Furthermore, antibodies to PCDH7 were detected in both the serum and kidney biopsy tissue from individuals with PCDH7-associated MN but not from controls. These findings suggest that PCDH7-associated MN defines a distinct type of MN.

Background
Membranous nephropathy (MN) results from deposition of antigen-antibody complexes along the glomerular basement membrane (GBM). PLA2R, THSD7A, NELL1, and SEMA3B account for 80%–90% of target antigens in MN.

Methods
We performed laser microdissection and mass spectrometry (MS/MS) in kidney biopsies from 135 individuals with PLA2R-negative MN, and used immunohistochemistry/immunofluorescence and confocal microscopy to confirm the MS/MS finding, detect additional cases, and localize the novel protein. We also performed MS/MS and immunohistochemistry on 116 controls and used immunofluorescence microscopy to screen biopsy samples from two validation cohorts. Western blot and elution studies were performed to detect antibodies in serum and biopsy tissue.

Results
MS/MS studies detected a unique protein, protocadherin 7 (PCDH7), in glomeruli of ten (5.7%) PLA2R-negative MN cases, which also were negative for PLA2R, THSD7A, EXT1/EXT2, NELL1, and SEMA3B. Spectral counts ranged from six to 24 (average 13.2 [SD 6.6]). MS/MS did not detect PCDH7 in controls (which included 28 PLA2R-positive cases). In all ten PCDH7-positive cases, immunohistochemistry showed bright granular staining along the GBM, which was absent in the remaining cases of PLA2R-negative MN and control cases. Four of 69 (5.8%) cases in the validation cohorts (all of which were negative for PLA2R, THSD7A, EXT1, NELL1, and SEMA3B) were PCDH7-positive MN. Kidney biopsy showed minimal complement deposition in 12 of the 14 PCDH7-associated cases. Confocal microscopy showed colocalization of PCDH7 and IgG along the GBM. Western blot analysis using sera from six patients showed antibodies to nonreduced PCDH7. Elution of IgG from frozen tissue of PCDH7-associated MN showed reactivity against PCDH7.

Conclusions
MN associated with the protocadherin PCDH7 appears to be a distinct, previously unidentified type of MN.
Depositional Environment of Fine-Grained Sedimentary Rocks of the Sinamar Formation, Muara Bungo, Jambi
Authors: Zajuli M. Heri Hermiyanto , Panggabean H. 
Published: April 2021

DOI: 10.17014/ijog.v8i1.153The research area is situated in the northwestern side of South Sumatra Basin, which is a part of Muara Bungo Regency, Jambi Province. The Oligocene Sinamar Formation consists of shale, claystone, mudstone, sandstone, conglomeratic sandstone, and coal-seam intercalations. This research was focused on fine sedimentary rock of Sinamar Formation, such as shale, claystone, and mudstone. Primary data were collected from SNM boreholes which have depths varying from 75 m up to 200 m, and outcrops that were analyzed by organic petrographic method, gas chromatography-mass spectrometry (GC-MS) of normal alkanes including isoprenoids, and sterane. The dominant maceral group is exinite, composed of alginite (3.4 - 18%), and resinite (1.6 - 5.6%), while vitrinite maceral consists of tellocolinite 0.4 - 0.6%, desmocollinite 0.4%, and vitrodetrinite 8.4 - 16.6%. Organic petrography and biomarker analyses show that organic materials of shales were derived from high plants and algae especially Botrycoccus species. Botrycoccus and fresh water fish fossil, found in the shale indicate a lacustrine environment.
Clinical profile and outcome of snakebite poisoning in sub-district hospital Akhnoor
Authors: Sharma Chandan , Badyal Ashima , Kumar Sanjeev 
Published: April 2021

Background: In a predominantly agricultural country like India, with rich in flora and fauna, long rainy season and rural background, the encounter between man and snake is a frequent occurrence. Viper-bites are more common than other poisonous snakebites. Only the cases of snakebite with severe envenomation reach the health care centers. This study was planned to analyze the presentation of complaints and symptoms in snake bite patients.Methods: This ‘record based descriptive observational study’ was carried out over a period of 6 months from May 2020 to November 2020 in Sub District hospital Akhnoor. A total of 96 patients with a history of snake bite and signs of envenomation were included in this study. Results: Incidence was higher among people from rural background (86.46%), more among males (67.71%) and highest among farmers (53.13%) and labourers (16.67%). Maximum no of snakebites took place during night and on the lower parts of body, mainly legs (54.17%); while the period of the study contained the peak rainy season of the region. As many as 39.58% of victims didn’t receive any kind of formal first aid at all and as many as 48.96% patients reported after a minimum delay of 6 hours, which could be considered as potentially fatal. Chief complaints among the reported patients of snake bite were found to be: Local edema, bleeding, vomiting, drowsiness, ptosis and Ophthalmoplegia.Conclusions: There is an urgent and dire need for awareness and education among rural population about the hazards involved and treatment of snakebite.
O BRINCAR NO HOSPITAL: UMA SELF DE ENFERMEIROS QUE ATUAM EM UNIDADE PEDIÁTRICA.
Authors: Fontes Esteves Arinete Veras , Santana de Melo Lílian Dornelles , Da Silva Sabino Aderlaine , Gusmão da Silva Marcos Vinícius , Cristino Joseir Saturnino , Pessoa Rocha Ellen 
Published: April 2021

Objetivo: Conhecer a importância da atividade do brincar sob a ótica do enfermeiro durante o cuidado à criança hospitalizada. Metodologia: Estudo descritivo-exploratório prospectivo, de abordagem qualitativa, realizado entre dezembro de 2014 a janeiro de 2015, com cinco enfermeiras que atuam em uma unidade pediátrica de referência da região Norte. Da análise, emergiram as categorias temáticas: “A importância do brincar no olhar do enfermeiro”, “O conhecimento sobre a regulamentação do brincar”, “O direito de brincar e o cuidar brincando”, “O brincar no ensino da graduação” e “Obstáculos para inclusão da brincadeira no assistir a criança hospitalizada”. Resultados: As enfermeiras reconhecem a importância do brincar no hospital, o qual proporciona momentos de distração e alegria, que trazem benefícios e diminuição do estresse ocasionado pela hospitalização. Conclusão: O brincar em clínica pediátrica deve ser uma atividade inserida no plano de cuidados dos enfermeiros.
Alteraciones atípicas del líquido cefalorraquídeo en el Síndrome de Guillain-Barré: Reporte de casos.
Authors: Zelada-Ríos Laura , Guevara-Silva Erik , Castro-Suarez Sheila , Caparó-Zamalloa César , Meza-Vega María 
Published: April 2021

El síndrome de Guillain-Barré se caracteriza por presentar una disociación albúmino-citológica en la mayoría de pacientes. La presencia de pleocitosis o hipoglucorraquia puede alejar el diagnóstico, por lo que se recomienda descartar, principalmente, causas infecciosas. Se presentan tres casos cuyos estudios de líquido cefalorraquídeo mostraron pleocitosis linfocítica e hiperproteinorraquia persistente y uno de ellos, además, hipoglucorraquia; fue solamente en análisis posteriores que los tres pacientes presentaron la clásica disociación albuminocitológica. El estudio neurofisiológico en todos ellos demostró asimismo un compromiso axonal. Las alteraciones atípicas en el contexto de parálisis flácida aguda justificarían repetir el análisis de líquido cefalorraquídeo y descartar otras etiologías, pero sin posponer en modo alguno el tratamiento.
Triads and The Abilities of Transnational Organized Crime to Prevail
Authors: Saadah Kholifatus 
Published: April 2021

AbstrakTransnational Organized Crime (TOC) adalah bentuk luas dari organisasi kejahatan yang dibentuk oleh sekelompok orang di wilayah tertentu. Dengan adanya globalisasi, perkembangan dari sebuah TOC bisa melewati batas-batas negara dan bahkan balik mengancam entitas dari negara tersebut. Satu hal yang membedakan TOC dengan organisasi kejahatan yang lain adalah struktur dan pola aktivitasnya yang rapi, seperti organisasi transnasional pada umumnya. Salah satu TOC yang melegenda sampai dengansekarang adalah kelompok mafa dari Cina, Triad. Sebagai organisasi kejahatan yang berumur panjang, berdiri sejak Dinasti Qing pada 1760-an, Triad memiliki banyak cabang yang tersebar di wilayah dengan populasi etnis Cina yang besar selain Cina sendiri seperti Taiwan, Hongkong, Singapura, Amerika Serikat dan Inggris. Melalui tulisan ini, penulis berusaha menjelaskan mengenai perkembangan Triad dari yang awalnya kelompok mafa menjadi kelompok yang memiliki tujuan bisnis seperti korporasi. Langgengnya kekuatan Triad sebagai sebuah organisasi kejahatan disebabkan juga banyaknya celah, baik diinstitusi domestik maupun institusi internasional. Celah tersebut dimanfaatkan secara baik oleh TOC untuk terus-menerus beraktivitas sampai sekarang.Kata-Kata Kunci: Organisasi Kejahatan Transnasional, TRIAD, Globalisasi, Mafia, KorporasiTransnational Organized Crime (TOC) is a widespread form of criminal organization formed by a group of people in a particular region. Because of globalization, the development of a TOC can cross the borders of the country and even turn against the entity of the state.One thing that distinguishes TOC from other crime organizations is, its neat structure and pattern of activities, such as transnational organizations in general. One of the legendary TOCs is the mafa group from China, Triad. As a long-lived crime organization, establishedsince the Qing Dynasty in the 1760s, Triad has many branchess cattered in areas with large Chinese ethnic populations other than China itself such as Taiwan, Hong Kong, Singapore, the United States and Britain. Through this paper, I’ll try to explain the development of the Triads, from a group that was originally a mafa to a group that has business objectives like a corporation. The lasting power of the Triads as a criminal organization is supported by the gap which happened in domestic and international institution. This gap is used properly by TOC to continue their activities until now.Keywords: Transnational Organized Crime, TRIAD, Globalization, Mafa, Corporation
The Perception of Cia-Cia Tribe Students in Southeast Sulawesi Toward South Korea: The Case of Education Aid for Cia-Cia Language Preservation
Authors: Mulyaman Darynaufal , Virgianita Asra , Candra Darang Sahdana 
Published: April 2021

Since the beginning of the "Korean Wave" in the 2010s, Korean language and the Hangeul alphabet are integral parts in the dissemination of South Korea's soft power across the world. For instances, the South Korean government provided support for the preservation of Cia-Cia language of Cia-Cia ethnic group in Bau-Bau, Southeast Sulawesi, Indonesia - a program that was originally initiated by Hunminjeongeum Society Foundation in 2008. This study examines the perception of students with Cia-Cia ethnic background toward South Korea. Questionnaire surveys and interviews are used to further look into their perceptions. This study, that started from a thesis, finds that the students who participated in the program have positive perceptions of South Korea. Furthermore, the demonstration of Korean culture and the use of Korean language become more apparent in the students’ daily life, creating a new social reality in the local community. Such finding suggests that international assistance has effectively become a part of South Korea's soft power expansion strategy.Keywords: Cia-Cia, Language, Foreign Aid, Korea, PerceptionSejak merebaknya "Korean Wave" pada dekade 2010-an, Bahasa Korea dan Aksara Hangeul adalah bagian integral dalam penyebaran soft power Korea Selatan di seluruh dunia. Pemerintah Korea Selatan, misalnya, memberikan dukungan untuk pelestarian bahasa Cia-Cia dari Suku Cia-Cia di Bau-Bau Sulawesi Tenggara, Indonesia - sebuah program yang awalnya diprakarsai oleh Yayasan Hunminjonggeum Society pada tahun 2008. Studi ini mengukur persepsi siswa dengan latar belakang etnis Cia-Cia terhadap Korea Selatan. Survei kuesioner dan wawancara digunakan untuk melihat lebih jauh persepsi mereka. Studi ini berangkat dari tesis yang dilakukan dan menemukan bahwa siswa-siswa yang terlibat dalam program tersebut memiliki persepsi yang positif tentang Korea Selatan. Selain itu, peragaan budaya Korea dan penggunaan Bahasa Korea menjadi lebih jelas dalam kehidupan sehari-hari siswa-siswa tersebut sehingga menciptakan realitas sosial baru di komunitas lokal. Temuan tersebut menunjukkan bahwa bantuan internasional dapat dan telah secara efektif digunakan sebagai strategi ekspansi soft power Korea Selatan. Kata-kata Kunci: Cia-Cia, Bahasa, Bantuan Luar Negeri, Korea, Persepsi
THE EFFECT OF RETENTION FORMS ON ACRYLIC BASE SURFACES ON ADHESIVE STRENGTH OF DENTURE RELINER MATERIALS
Authors: Prawesthi Endang , Tirta Handoko , Rahmaniawati Rahmaniawati 
Published: April 2021

Background: Acrylic prosthesis that are used for a long time are often not sticky anymore and easy off. Treatment that can be done is relining, which is resurfacing with new material addition to prosthesis surface in contact with mucosa. One of materials used is denture reliner made of acrylic material (heat and self cured acrylic). The successful use of this reliner material depends on the retention of acrylic base surface. There is some retentions namely bur strokes, ethyl acetate and Al2O3 sandblast. Purpose: To determine effect of retention in form of bur strokes, Ethyl Acetate and Al2O3 sandblast on acrylic bases surface on adhesive strength of reliner denture material. Method: Research using samples in form of acrylic blocks with a length of 80mm, a large block width of 10 x10 mm and small block of 10 x 8 mm, reliner material thickness is 3 mm. Total of 64 samples were divided into 2 groups based on reliner materials (heat and self) and each group was divided into 4 groups based on treatment (control, bur stroke, ethyl acetat and Al2O33 sandblast). All samples were subjected to tensile test using UTM to obtain tensile strength values. Result: Heat CA tensile strength value was greater than self CA and the two groups were significantly different (p<0.05), while lowest tensile strength value in control group followed by ethyl acetate and largest value in Al2O3 sandblast and almost all groups were significantly different (p<0.05). Conclusion: Heat CA is more adhesive than self CA and retention on acrylic surface is proven to be effective in increasing adhesive strength of acrylic surface against denture reliner.
Prevention of Unplanned Surgery Due to Delayed Diagnosis of Occult Ipsilateral Femoral Neck Fractures Associated with Femoral Shaft Fractures
Authors: Park Young-Chang , Song Hyung-Keun , Yang Kyu-Hyun 
Published: April 2021

Background:
The purpose of the present study was to investigate the effectiveness of using the computed tomography (CT) capsular sign with lipohemarthrosis of the hip joint as a selective indicator for preoperative magnetic resonance imaging (MRI) of the hip or prophylactic fixation of the ipsilateral femoral neck for the prevention of unplanned surgery due to delayed diagnosis of occult ipsilateral femoral neck fractures in patients with high-energy femoral shaft fractures.

Methods:
We evaluated the CT capsular sign with lipohemarthrosis in patients with a high-energy femoral shaft fracture without a preoperative diagnosis of an ipsilateral femoral neck fracture. The CT capsular sign with lipohemarthrosis was considered positive when the side-to-side difference in anterior capsular distension was >1 mm and lipohemarthrosis was seen on soft-tissue-window CT images. A positive CT capsular sign with lipohemarthrosis prompts preoperative hip MRI or prophylactic femoral neck fixation with a reconstruction nail.

Results:
One hundred and fifty-six consecutive patients were included. Eight patients were preoperatively diagnosed with a displaced or hairline ipsilateral femoral neck fracture, whereas the remaining 148 patients showed no ipsilateral femoral neck fracture on radiographs and bone-window CT images. On soft-tissue-window CT images, 29 (19.6%) of the 148 patients had a positive CT capsular sign with lipohemarthrosis. We performed preoperative MRI for 3 patients; in the remaining 26 patients, prophylactic femoral neck fixation was performed with a reconstruction nail. We identified 5 occult ipsilateral femoral neck fractures among the 29 patients with a positive sign: 2 on preoperative MRI scans, 2 on immediate postoperative radiographs, and 1 on radiographs made 6 weeks postoperatively. In 119 patients with a negative sign, no occult ipsilateral femoral neck fracture was identified. All occult ipsilateral femoral neck fractures healed without further displacement of the femoral neck. Consequently, additional unplanned surgery for delayed diagnosis of occult ipsilateral femoral neck fracture was not required.

Conclusions:
The use of the CT capsular sign with lipohemarthrosis as a selective indicator for preoperative hip MRI or prophylactic femoral neck fixation with a reconstruction nail in patients with high-energy femoral shaft fractures is effective for preventing unplanned surgery due to delayed diagnosis of occult ipsilateral femoral neck fractures.

Level of Evidence:
Diagnostic Level I. See Instructions for Authors for a complete description of levels of evidence.
Bacterial Colonization and TH17 Immunity are Shaped by Intestinal Sialylation in Neonatal Mice
Authors: Irons Eric E , Gomez Eduardo Cortez , Andersen Valerie L , Lau Joseph 
Published: April 2021

Abstract
Background: Interactions between the neonate host and its gut microbiome are central to the development of a healthy immune system. However, the mechanisms by which animals alter early colonization of microbiota for their benefit remain unclear. Host-derived carbohydrates, which can serve as metabolic substrates for the expansion of specific commensal and pathogenic bacteria, are one method by which the host may influence interspecies competition in the microbiome. Here, we investigated the role of early-life expression of the α2,6- sialyltransferase ST6GAL1 in microbiome phylogeny and mucosal immunity. Methods: Fecal, upper respiratory, and oral microbiomes of pups expressing or lacking St6gal1 were analyzed by 16S rRNA sequencing. Pooled fecal microbiomes from syngeneic donors were transferred to antibiotic-treated wild-type mice, before analysis of recipient mucosal immune responses by flow cytometry, RT-qPCR, microscopy, and ELISA. Intestinal sialylation was characterized by RT-qPCR, immunoblot, microscopy, and sialyltransferase enzyme assays in genetic mouse models at rest or with glucocorticoid receptor modulators. Results: At weaning, the fecal microbiome of St6gal1 -KO mice exhibited reductions in Clostridiodes, Coprobacillus, and Adlercreutzia, but increased Helicobacter and Bilophila . Transfer of St6gal1- KO microbiome induced a mucosal Th17 response, with expression of T-bet and IL-17, and IL-22-dependent gut lengthening. ST6GAL1 was found to be highly expressed in duodenal enterocytes between birth and weaning, driving temporary sialylation of the associated glycocalyx and secretion of active ST6GAL1 into the intestinal lumen. Expression was mediated by the P1 promoter of the St6gal1 gene, which was efficiently inhibited by dexamethasone. Conclusions: Intestinal sialylation by the sialyltransferase ST6GAL1 in the neonatal period is a developmentally regulated host mechanism coordinating bacterial colonization in the early gut microbiome. The inability to produce α2,6-sialyl ligands results in microbiome-dependent Th17 inflammation, highlighting a pathway by which intestinal epithelium regulates mucosal immunity. Considering the prevalence of intestinal fucosylation in adult animals, sialic acid may promote an early stage of ecological succession in the developing gut. Trial registration: N/A
Functional and diffusion MRI reveal the neurophysiological basis of neonates’ noxious-stimulus evoked brain activity
Authors: Baxter Luke , Moultrie Fiona , Fitzgibbon Sean , Aspbury Marianne , Mansfield Roshni , Bastiani Matteo , Rogers Richard , Jbabdi Saad , Duff Eugene , Slater Rebeccah 
Published: April 2021

Abstract
Understanding the neurophysiology underlying neonatal responses to noxious stimulation is central to improving early life pain management. In this neonatal multimodal MRI study, we use resting-state and diffusion MRI to investigate inter-individual variability in noxious-stimulus evoked brain activity. We observe that cerebral haemodynamic responses to experimental noxious stimulation can be predicted from separately acquired resting-state brain activity (n=18). Applying this prediction model to independent Developing Human Connectome Project data (n=215), we identify negative associations between predicted noxious-stimulus evoked responses and white matter mean diffusivity. These associations are subsequently confirmed in the original noxious stimulation paradigm dataset, validating the prediction model. Here, we observe that noxious-stimulus evoked brain activity in healthy neonates is coupled to resting-state activity and white matter microstructure, that neural features can be used to predict responses to noxious stimulation, and that the dHCP dataset could be utilised for future exploratory research of early life pain system neurophysiology.
Polymicrobial and monomicrobial necrotizing fasciitis: clinical, laboratory, radiology, pathological hallmark and differences, a retrospective analysis
Authors: Naaman Eviatar , Shiber Shachaf , Hadar Daskin-Bitan , Yahav Dafna , Bishara Jihad , Sagy Iftach , Granat Nadav , Drescher Micheal 
Published: April 2021

Abstract
Background: Necrotizing fasciitis (NF) is a life-threatening infection with high morbidity and mortality rates which should be diagnosed and treated with surgical and antibiotic therapy. Many studies have addressed NF and its subtypes, but few have reviewed the clinical, radiological and pathological differences between the poly-microbial and the mono-microbial groups. Objective: The objective of our study is to describe a relatively large cohort of patients with NF and study and compare the clinical, radiological and pathological differences between the poly-microbial (Pm) and the mono-microbial (Mm) groups.Methods: The charts of hospitalized patients with NF diagnosis from 2002-2019 at the Rabin Medical Center were reviewed. The primary outcome was all-cause mortality at 90 days, secondary outcomes included duration of hospitalization, intensive care unit (ICU) admission, LRINEC score and the need for vasopressor use. Results: 81 patients with NF were included in the study, 54(66.6%) had Mm growth and 27(33.3%) had Pm growth. There were no significant differences between the two groups in the 90 days mortality, and moreover in hospital mortality was also insignificantly different. In a multivariate analysis, we found that 90 days mortality was more prevalent in the Mm group compared to Pm group. In addition, we found that in hospital mortality, ICU admission and vasopressors use were more frequent among the Mm-group compared to the Pm-group. Conclusions: our study is the first to compare the differences between the two most prevalent entities of NF. The results demonstrate better prognosis for Pm-NF, with minimal ICU stay, lower mortality, and lower use of vasopressors.