bluuebunny/crossref_metadata_2025_split huggingface dataset:
Internet Mentalization Informed Video Feedback (MI-VF) Intervention to Improve Parental Sensitivity in Mother-Infant Dyads with Maternal Depressive Symptoms: Study Protocol for a Randomized Controlled Feasibility Trial
Authors: Huber Marcia Olhaberry , Leyton Fanny , Moran Javier , León María José , Sieverson Catalina , Muzard Antonia , Honorato Carolina , Ensink Karin , Malberg Norka , Luyten Patrick , Costa-Cordella Stefanella 
Published: May 2021

Abstract
Background: Depressive symptoms in the postpartum period are a highly prevalent mental health problem. COVID-19 restrictions have increased maternal clinical symptoms during the postpartum period, leading to a high demand for mental health assistance and the need for cost-effective care adjusted to this new context. Video feedback (VF) has been a particularly cost-effective strategy in early interventions focused on maternal sensitivity. However, these interventions usually do not include mentalization as one of the critical elements involved in sensitive and attachment relationships. Also, these interventions are generally carried out in face-to-face settings. This study's main goal is to evaluate the feasibility and acceptability of a brief internet mentalization informed with VF to improve the sensitive response in mother-baby dyads with postpartum depressive symptoms attended in primary health centers.Methods/design: This randomized feasibility trial will include 60 mother-baby dyads. Participants will be randomized and allocated in a 1:1 ratio. A random number sequence will be computer-generated in varying block sizes (2&4) and stratified by center. The intervention aims to improve maternal sensitivity and mentalizing by using a model based on video-feedback methodology in a virtual modality. The intervention consists of four sessions plus one pre-intervention assessment session and psychoeducational cards. Control dyads will receive only psychoeducational cards once a week. Additionally, dyads from both groups will receive the health center’s treatment as usual. A MI-VF manual guides each intervention session, but its application is tailored to each dyad, VF therapists will receive one weekly group supervision session. Data will be collected at baseline, 4 weeks and 3 months follow-up. The main goal of this trial is to assess feasibility outcomes (recruitment rate and data completion) and change in maternal sensibility.Discussion: This study will inform parameters for the future implementation of a large randomized controlled clinical trial, contributing to the current scarce evidence on mentalization informed interventions that use VF as their primary intervention strategy. Also, this study will provide a cost-effective internet-based intervention model, feasible to be implemented in public health settings.Trial registration: NCT04748731
The Saliva Cortisol and Amylase Levels Related With Stress Response Compared by Different Analytical Methods
Authors: Şemsi Rabia , Kökbaş Umut , Arslan Burak , Dinçel Aylin Sepici , Ergünol Erdal , Kayrın Levent 
Published: May 2021

Abstract
Sampling of salivary cortisol and salivary amylase is non-invasive and important for the evaluation of the hypothalamic-pituitar-adrenal axis funciton and stress levels. This study aims to compare the values of the cortisol and salivary amylase measured by using three diffeent analytical methods and discuss the comparison of stress levels of samples.Young adults (n =23) saliva samples were collected between 08.00 and 09.00 am, noon at 12.00 (before exam) and 14.00-15.00 (after unaware exam) during the first 48 hours, no freezing / thawing was done. Salivary cortisol and salivary amylase levels in subjects were measured by three different analytical methods (ELISA, Chemiluminescence and Biosensor).Comparison of method between ELISA and biosensor in determining salivary kortizol levels showed a good correlation y= 2.971+0.748x (R2=0.839). Salivary amylase concentrations were found only by ELISA method.The detection/information effectiveness as compared with conventional determination method ELISA, considering the low cost, fast results and specificity characteristics biosensor can be offered as an alternative method.
Investigation of ZnO nanotubes array as an antireflective coating
for planar CZTS/ZTO solar cell
Authors: melliti adnen 
Published: May 2021

Abstract
We present a theoretical investigation of ZnO nanotubes square periodic array as an antireflective coating for a planar solar cell including Kesterite as a p-type absorber and Zinc Tin Oxide as an n-type buffer. The method of the calculation is rigorous coupled wave analysis. We found that this coating leads to a considerable reduction of the loss of the ideal short circuit current density by about 80 %. The optimal values of the nanotube diameter, nanotube height, array period and the absorber thickness are 438 nm, 150 nm, 438 nm and 1.5 µm respectively. The study of the variation of the loss of the ideal short circuit current density with the incident angle of the sunlight shows that the NTs array still has good anti-reflection characteristics as long as the incident angle doesn’t exceed 60°.
Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus-Derived Mesenchymal Stem Cells
Authors: Zhang Hua , Li Wei , Zhang Shengquan , Li Jie , Han Letian , Chen Haoyu , Wang Ziyu , Shen Cailiang , Zhang Yinshun , Tao Hui 
Published: May 2021

Abstract
Background:The expansion and differentiation of nucleus pulposus-derived mesenchymal stem cells（NPMSCs） are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine.However, the harsh microenvironment within the intervertebral disc is the main limitation of its regeneration.The osmolarity of the intervertebral disc is higher than that of other tissues, which has an important influence on the biological characteristics of NPMSCs. In this study,we observed the effect of different osmolarities on the biological characteristics of human normal NPMSCs cultured in vitro and explored the role of osmolarity in intervertebral disc degeneration. Methods: NPMSCs were isolated from the lumbar intervertebral disc (Pfirrmann I or II) of patients with lumbar fractures by collagenase digestion, and nucleus pulposus-derived stem cells were isolated, cultured and passaged in vitro. The isolated cells were identified by immunophenotype and multilineage differentiation. Then, the cells were cultured under conditions with different osmotic pressures. The cell proliferation rate, apoptosis rate ,cell cycle status and cell senescence were detected, and the expression of stemness genes (Nanog, Notch1, Jag1, OCT4), functional genes (Aggrecan, Collagen II) and the P16INK4A and RB genes was detected. siRNA transfection was used to determine the role of the P16INK4A/RB signaling pathway in the effect of changes in osmotic pressure on cells.Results: The experimental results demonstrated that the isolated cells met the standards of the International Society for Cell Therapy. The cells we isolated and cultured from the intervertebral discs are NPMSCs. Our work showed that low and high osmotic pressure can inhibit cell proliferation, promote cell apoptosis, block cell cycle progression, promote cell senescence,downregulate the expression of stemness genes (Nanog, Notch1, Jag1, OCT4) and functional genes (Aggrecan, Collagen II), and upregulate the expression of the P16INK4A and Rb genes.The transfection results showed that after knocking down P16INK4A gene, hypotonic or hypertonicity had no effect on expression of genes (Aggrecan, Collagen II,Rb).Conclusion: The change in osmotic pressure has an important effect on the biological activity of NPMSCs, and this effect may occur through the p16INK4A/Rb pathway.This study provides a theoretical basis for the future treatment of intervertebral disc degeneration.
Active Cytoskeletal Composites Display Emergent Tunable Contractility and Restructuring
Authors: Lee Gloria , Leech Gregor , Lwin Pancy , Michel Jonathan , Currie Christopher , Rust Michael , Ross Jennifer , McGorty Ryan , Das Moumita , Robertson-Anderson Rae 
Published: May 2021

Abstract
The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both active actomyosin dynamics and actin-microtubule interactions are key to the cytoskeleton’s versatility and adaptability, an understanding of their interplay is lacking. Here, we couple microscale experiments with mechanistic modeling to elucidate how connectivity, rigidity, and force-generation affect emergent material properties in in vitro composites of actin, tubulin, and myosin. We use time-resolved differential dynamic microscopy and spatial image autocorrelation to show that ballistic contraction occurs in composites with sufficient flexibility and motor density, but that a critical fraction of microtubules is necessary to sustain controlled dynamics. Our active double-network models reveal that percolated actomyosin networks are essential for contraction, but that networks with comparable actin and microtubule densities can uniquely resist mechanical stresses while simultaneously supporting substantial restructuring. Our findings provide a much-needed blueprint for designing cytoskeleton-inspired materials that couple tunability with resilience and adaptability.
(Murradambirra Dhangaang - Make Food Secure) Aboriginal Community and Stakeholder Perspectives on Food Insecurity in Urban and Regional Australia: A Qualitative Study
Authors: Sherriff Simone , Kalucy Deanna , Tong Allison , Naqvi Nawazish , Nixon Janice , Eades Sandra , Ingram Tangerene , Slater Kym , Dickson Michelle , Lee Amanda , Muthayya Sumithra 
Published: May 2021

Abstract
Background:

Food insecurity affects one in five Aboriginal and Torres Strait Islander people residing in non-remote environments. Inequalities in diet contribute to the differential impact on diet-sensitive chronic diseases and the related burden of disease among Aboriginal and Torres Strait Islander people. This study aimed to describe Aboriginal community and stakeholder perspectives on food insecurity to get a better understanding of the key factors driving this issue and recommendations for potential solutions in urban and regional Aboriginal communities in Australia.
Methods:

Semi-structured interviews were conducted with 44 participants who were purposively selected. This included Aboriginal people in two communities and both Aboriginal and non-Aboriginal stakeholders from local food relief agencies, food suppliers, schools, and government. A conceptual framework was developed from food insecurity literature and we used the sensitizing concepts of availability, affordability, accessibility and acceptability or the lack thereof of healthy food to elicit interview responses. Interview transcripts were analysed thematically.
Results:

All participants felt strongly that food insecurity was a major problem experienced in the local Aboriginal communities. Five core areas impacting on food security identified: trapped in financial disadvantage; gaps in the local food system; limitations of non-Aboriginal food relief services; on-going impacts of colonization; and maintaining family, cultural and community commitments and responsibilities.
Conclusions:

This study found Aboriginal families in urban and regional Australia are experiencing food insecurity on a regular basis, which is impacted by a range of socio-economic, environmental, systemic and cultural factors, as reported by the participants. Our findings highlight the need to address system level changes in the food environment and acknowledge Aboriginal culture and food preferences while considering the development of programs to alleviate food insecurity among urban and regional communities.
The Efficacy and Safety of Massage for Treatment of Functional Constipation in Children: Protocol for Meta-Analysis of Randomized Controlled Trials
Authors: Cao Xue , Wu Jia-ping , Bai Yi-fan , Zhang Yan , Zhang Jia-jia , Liu Ya-li , Liu Jia 
Published: May 2021

Abstract
Background: Constipation is one of the most common diseases in children and it is also a significant healthcare burden, more than many other common childhood diseases. Massage has gained increased popularity for the management of constipation. However, there is a lack of supportive evidence on the efficacy of massage for constipation in children. This systematic review aims to collect and critically appraise all the available evidence about the efficacy and safety of massage for functional constipation (FC) in children. Methods/Design: The electronic databases of Embase, PubMed, and the Cochrane Library, Web of Science, China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database, Wanfang Database (Wanfang), Chinese Scientific and Journal Database (VIP) will be searched from the establishment to February 2021 for randomized controlled trials investigating massage for treatment of functional constipation in children. Papers not in English or Chinese language were excluded. Two independent reviewers will conduct studies retrieval, remove duplication, screen, extract data, evaluate quality, and analyze data by NoteExpress (Version 3.2) and RevMan (Version 5.3.5 software (the Cochrane Collaboration, Oxford, England). We will conduct the meta-analysis to synthesis the evidence for each outcome. The primary outcome included: 1) Overall response rate (according to stool frequency, time of defecation interval, stool consistency, difficult defecation, sense of incomplete evacuation, abdominal pain and bloating); 2) The mean number of bowel movements in the fourth week. Secondary outcome measures included 1) The symptom integrals; 2) Adverse events; 3) Recurrence rate. Cochrane Handbook for Systematic Reviews of Interventions will be used to assess the risk of bias, and GRADE will be used to access the confidence in cumulative evidence. The protocol followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols.Discussion: The findings of this meta-analysis will be important to clinicians and patients regarding the use of massage in childhood functional constipation.Study registration: PROSPERO registration number: CRD42021237173
Novel Variants of Avian Paramyxoviruses 13 in Wild Migratory Waterfowl Reveals 2 Low Evolutionary Rate
Authors: Fei Yidong , Ding Zhuang , Cong Yanlong , Chen Jianchun , Yin Renfu 
Published: May 2021

Abstract
Avian paramyxovirus 13 (APMV-13), formerly known as Avian orthoavulavirus 13, is found scatteredly in wild birds around the world. Although four complete genome sequences of APMV-13 had been identified since the first discovery in Japan in 2003, the information available on the genetic variation and biological characteristics of APMV-13 is still limited. In the present study, we isolated six APMV-13 strians from fecal samples of wild migratory waterfowls during annual (2013-2018) viral surveillance of wild bird populations from wetland in China. The phylogenetic analyses showed that they had very close relationship in HN and F genes. The molecular clock estimations also showed a low evolutionary rate of APMV-13. However, there are obvious sequence differences among these strains. Bean goose/Hubei/V97-1/2015 is 1,953 nt in size (ORF, 1, 776 nt), which is a unique size and longer than other reported APMV-13 strians. Additionally, Swan goose/Hubei/VI49-1/2016 has four repeats of conserved sequences “AAAAAT” in the 5’-end trailer, which is unprecedented in the APMV-13. These findings reveal the existence of novel variants within APMV-13, which highlights the importance of continuous monitoring the specific species of APMVs.
Estimating the Genetic Parameters of Flowering-Time Related traits in a Miscanthus Sinensis population tested with a staggered-start design
Authors: HOU Wei , RAVERDY Raphaël , MIGNOT Emilie , ARNOULT Stéphanie , GIAUFFRET Catherine , BRANCOURT-HULMEL Maryse 
Published: May 2021

Abstract
There is a growing interest in the cultivation of miscanthus on marginal land, but biomass yields are much lower there than on good farming land. Therefore, understanding what causes such instability is of primary interest for breeding later-flowering but stable miscanthus genotypes. Our objectives were to estimate the genetic parameters -genetic variance and genetic heritability- and genetic correlations for flowering-time-related traits in a biparental Miscanthus sinensis diploid population, and to divide the year effect into both age and climate effects using a staggered-start design. The population was established with single plants organized in a staggered-start design and consisted of two genotype groups established twice, in 2014 and 2015, with a total of 159 genotypes and 82 common genotypes between the two groups. All plants were extensively phenotyped for different panicle and anther emergence traits in 2018 and 2019. All traits were delayed by about 20 days in 2019 compared to 2018, which was explained by climatic conditions that occurred before the floral transition, mainly by a 3°C decrease in maximal and minimal temperatures. When dividing the year interaction effect, the genotype × climate interaction was much higher than the genotype × age interaction. The climate effect not only caused a delay in the flowering time but also involved differential genotype behavior through climate × genotype interactions, which increased the corresponding genotype × climate interaction variance compared to the genotype × age interaction variance: the climate effect decreased the genetic parameters for all flowering-time related traits, up to 20 % for broad-sense heritability. Interestingly, all traits responded similarly to the climate effect, excepting the interval between the start of panicle emergence and that of anther appearance, for which the correlation coefficients were lower due to significant climate interactions, compared to genotype × age interactions. Therefore, M. sinensis breeding for flowering-time related traits must be conducted under contrasted climates in order to select more stable genotypes.
U (VI) tolerance affects Shewanella sp. RCRI7 biological responses: growth, morphology and bioreduction ability
Authors: Zarei Mahsa , Fatemi Faezeh , Mir-Derikvand Mohammad , Hosseinpour Hamzeh , Ghasemi Razieh 
Published: May 2021

Abstract
Native Shewanella sp. RCRI7 is recently counted as an operative bacterium in the uranium bioreduction. The aim of this study was to investigate the effect of uranium tolerance on the morphology and population of RCRI7 following its potential removal capacity in different time intervals. Accordingly, the bacterial growth and uranium removal kinetic were evaluated in aerobic TSB medium, uranium reducing condition (URC), aerobic uranium containing (AUC) and anaerobic uranium free (AUF) solutions, following evaluations of omcAB gene expressions. In addition, spectrophotometry analyses were performed in URC confirming the bioreduction mechanism. It was found that the bacteria can grow efficiently in the presence of 0.5 mM uranium anaerobically, unlike AUC and AUF solutions during 92 h incubation. The bacteria can kinetically remove a large amount of soluble uranium through adsorption pathway in the first 12 h in URC. This occurs in a circumstance that the removal process keeps on increasing via reduction lane up to 84 h, when the most amount of uranium removal has been indicated. In 92 h, the adsorbed uranium containing unreduced and reduced (U (IV) monomeric), was released to the solution due to either the increased pH or bacterial death. In AUC and AUF, improper conditions lead to reduced bacterial size and increased bacterial aggregations, however, membrane vesicles produced by the bacteria avoid the uranium incrustation in AUC.
Buccal mucosal graft urethroplasty for anterior urethral stricture, Experience from a low-income country
Authors: Taha Sami Mahjoub , Ahmed Musab Abdalla , Osman Yassin M. , Elemam Mohammed , Daffalla-Awadalla Mohamed 
Published: May 2021

Abstract
Background/purpose: This study was conducted to present our experience in urethral mucosal graft urethroplasty to repair urethral stricture, as the first experience in our context.Methods: This is a prospective hospital-based study that had been designed to review management outcomes of buccal mucosal graft urethroplasty for anterior urethral stricture from January 2017 to January 2019.Results: The total number of involved patients was 60. The success rate was found to be 90% (n= 54), while 6(10%) had a recurrence of stricture. Pain and pain combined bleeding from internal suture lines were the only early complication encountered in 50(83.3%) and 2(3.3%) patients, respectively. late complications occurred as follows 14(23.3%) patients had UTI, 12(20%) had wound infections, 8(13.3%) had changes in ejaculation, and decrease in intensity of orgasm, and 6(10%) had erectile dysfunction. One of the long-term complications was graft diverticulum in one case and was treated conservatively (in ventral on lay BMG).Conclusion: Improvement of the service in limited resources countries like Sudan and was reflected in the excellent outcome of BMG urethroplasty as treatment of anterior urethral stricture (success rate 90%).
Multilevel Mixed Effects Analysis of Individual and Community Factors Associated With Unmet Need for Contraception Among Married Women in Four East African Countries
Authors: Kabagenyi Allen , Wasswa Ronald , Henry Nsobya , Paulino Ariho , Sseninde Julius , Rusatira Jean Christophe 
Published: May 2021

Abstract
Background: Although use of contraceptives is an expression of a woman’s reproductive control, unmet need for contraception remains high and a public concern among married women in East Africa. Limited literature has explored the associated factors in the region. This study examined the individual and community level factors associated with unmet need for contraception among married women in Burundi, Rwanda, Tanzania and Uganda. Methods: The study utilized data from the four most recent demographic and health surveys on the following selected samples of women in Burundi (8767), Rwanda (6472), Tanzania (7417) and Uganda (10288). Analyses were conducted using multilevel mixed effect logistic regressions with random community and country level effects.Results: Findings showed that: 20%, 22%, 28% and 33% of the married women in Rwanda Tanzania, Uganda and Burundi respectively had unmet need for contraception. Young married women, low education level, low wealth quintile, higher number of living children, couples that are not staying together, husband’s desire for more children, history of child death, women who are not working, no access to family planning messages, long distances to health facilities, and husbands as decision-makers on women’s health care were associated with high level of unmet need for contraception. Also, low community wealth, low community use of modern contraceptives, higher community age at marriage, higher community mean number of living children and lower community age at sexual debut were found to be significantly associated with high risk of unmet need for contraception in at least one of the countries. Conclusions: The findings suggest the need to design programs and policies that ensure awareness and access to modern contraceptive services among all disadvantaged communities and unique groups like couples who are not staying together through increased outreaches and increased mass media campaigns. In addition, concerted efforts aimed at raising the educational levels of people to bring about change in social and cultural norms that influence the husbands desire for higher number of children are much needed. Deliberate efforts of empowering women through education, job creation, and reproductive health decisions will significantly reduce the levels of unmet need for contraception in East Africa.