bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure of the class C orphan GPCR GPR158 in complex with RGS7-Gβ5
Authors: Jeong Eunyoung , Kim Yoojoong , Jeong Jihong , Cho Yunje 
Published: November 2021

AbstractGPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding domain and terminates Gαi/o protein signaling through the RGS7-Gβ5 heterodimer. Here, we report the cryo-EM structures of GPR158 alone and in complex with one or two RGS7-Gβ5 heterodimers. GPR158 dimerizes through Per-Arnt-Sim-fold extracellular and transmembrane (TM) domains connected by an epidermal growth factor-like linker. The TM domain (TMD) reflects both inactive and active states of other class C GPCRs: a compact intracellular TMD, conformations of the two intracellular loops (ICLs) and the TMD interface formed by TM4/5. The ICL2, ICL3, TM3, and first helix of the cytoplasmic coiled-coil provide a platform for the DHEX domain of one RGS7 and the second helix recruits another RGS7. The unique features of the RGS7-binding site underlie the selectivity of GPR158 for RGS7.
Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Authors: Cheng Hang , Sebaa Rajaa , Malholtra Nikita , Lacoste Baptiste , El Hankouri Ziyad , Kirby Alexia , Bennett Nigel C. , van Jaarsveld Barry , Hart Daniel W. , Tattersall Glenn J. , Harper Mary-Ellen , Pamenter Matthew E. 
Published: November 2021

AbstractNaked mole-rats are among the most hypoxia-tolerant mammals. During hypoxia, their body temperature (Tb) decreases via unknown mechanisms to conserve energy. In small mammals, non-shivering thermogenesis in brown adipose tissue (BAT) is critical to Tb regulation; therefore, we hypothesize that hypoxia decreases naked mole-rat BAT thermogenesis. To test this, we measure changes in Tb during normoxia and hypoxia (7% O2; 1–3 h). We report that interscapular thermogenesis is high in normoxia but ceases during hypoxia, and Tb decreases. Furthermore, in BAT from animals treated in hypoxia, UCP1 and mitochondrial complexes I-V protein expression rapidly decrease, while mitochondria undergo fission, and apoptosis and mitophagy are inhibited. Finally, UCP1 expression decreases in hypoxia in three other social African mole-rat species, but not a solitary species. These findings suggest that the ability to rapidly down-regulate thermogenesis to conserve oxygen in hypoxia may have evolved preferentially in social species.
Monthly direct and indirect greenhouse gases emissions from household consumption in the major Japanese cities
Authors: Long Yin , Jiang Yida , Chen Peipei , Yoshida Yoshikuni , Sharifi Ayyoob , Gasparatos Alexandros , Wu Yi , Kanemoto Keiichiro , Shigetomi Yosuke , Guan Dabo 
Published: November 2021

AbstractUrban household consumption contributes substantially to global greenhouse gases (GHGs) emissions. Urban household emissions encompass both direct and indirect emissions, with the former associated with the direct use of fossil fuels and the latter with the emissions embodied in the consumed goods and services. However, there is a lack of consistent and comprehensive datasets outlining in great detail emissions from urban household consumption. To bridge this data gap, we construct an emission inventory of urban household emissions for 52 major cities in Japan that covers around 500 emission categories. The dataset spans from January 2011 to December 2015 and contains 12,384 data records for direct emissions and 1,543,128 records for indirect emissions. Direct emission intensity is provided in g-CO2/JPY to facilitate both future studies of household emission in Japan, as well as act as a reference for the development of detailed household emission inventories in other countries.
The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein
Authors: LaFrance Benjamin J. , Cassidy-Amstutz Caleb , Nichols Robert J. , Oltrogge Luke M. , Nogales Eva , Savage David F. 
Published: November 2021

AbstractBacterial nanocompartments, also known as encapsulins, are an emerging class of protein-based ‘organelles’ found in bacteria and archaea. Encapsulins are virus-like icosahedral particles comprising a ~ 25–50 nm shell surrounding a specific cargo enzyme. Compartmentalization is thought to create a unique chemical environment to facilitate catalysis and isolate toxic intermediates. Many questions regarding nanocompartment structure–function remain unanswered, including how shell symmetry dictates cargo loading and to what extent the shell facilitates enzymatic activity. Here, we explore these questions using the model Thermotoga maritima nanocompartment known to encapsulate a redox-active ferritin-like protein. Biochemical analysis revealed the encapsulin shell to possess a flavin binding site located at the interface between capsomere subunits, suggesting the shell may play a direct and active role in the function of the encapsulated cargo. Furthermore, we used cryo-EM to show that cargo proteins use a form of symmetry-matching to facilitate encapsulation and define stoichiometry. In the case of the Thermotoga maritima encapsulin, the decameric cargo protein with fivefold symmetry preferentially binds to the pentameric-axis of the icosahedral shell. Taken together, these observations suggest the shell is not simply a passive barrier—it also plays a significant role in the structure and function of the cargo enzyme.
Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
Authors: Kennedy Ian W. , Tsimbouri P. Monica , Campsie Paul , Sood Shatakshi , Childs Peter G. , Reid Stuart , Young Peter S. , Meek Dominic R. M. , Goodyear Carl S. , Dalby Matthew J. 
Published: November 2021

Abstract
Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast progenitor cells. We show that 1000 Hz frequency, 40 nm amplitude vibration reduces osteoclast formation and activity in human mononuclear CD14+ blood cells. Additionally, this nanoscale vibration both enhances osteogenesis and reduces osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Further, we use metabolomics to identify Akt (protein kinase C) as a potential mediator. Akt is known to be involved in bone differentiation via transforming growth factor beta 1 (TGFβ1) and bone morphogenetic protein 2 (BMP2) and it has been implicated in reduced osteoclast activity via Guanine nucleotide-binding protein subunit α13 (Gα13). With further validation, our nanovibrational bioreactor could be used to help provide humanised 3D models for drug screening.
Assessment of CT to CBCT contour mapping for radiomic feature analysis in prostate cancer
Authors: Schmidt Ryder M. , Delgadillo Rodrigo , Ford John C. , Padgett Kyle R. , Studenski Matthew , Abramowitz Matthew C. , Spieler Benjamin , Xu Yihang , Yang Fei , Dogan Nesrin 
Published: November 2021

AbstractThis study provides a quantitative assessment of the accuracy of a commercially available deformable image registration (DIR) algorithm to automatically generate prostate contours and additionally investigates the robustness of radiomic features to differing contours. Twenty-eight prostate cancer patients enrolled on an institutional review board (IRB) approved protocol were selected. Planning CTs (pCTs) were deformably registered to daily cone-beam CTs (CBCTs) to generate prostate contours (auto contours). The prostate contours were also manually drawn by a physician. Quantitative assessment of deformed versus manually drawn prostate contours on daily CBCT images was performed using Dice similarity coefficient (DSC), mean distance-to-agreement (MDA), difference in center-of-mass position (ΔCM) and difference in volume (ΔVol). Radiomic features from 6 classes were extracted from each contour. Lin’s concordance correlation coefficient (CCC) and mean absolute percent difference in radiomic feature-derived data (mean |%Δ|RF) between auto and manual contours were calculated. The mean (± SD) DSC, MDA, ΔCM and ΔVol between the auto and manual prostate contours were 0.90 ± 0.04, 1.81 ± 0.47 mm, 2.17 ± 1.26 mm and 5.1 ± 4.1% respectively. Of the 1,010 fractions under consideration, 94.8% of DIRs were within TG-132 recommended tolerance. 30 radiomic features had a CCC > 0.90 and 21 had a mean |%∆|RF < 5%. Auto-propagation of prostate contours resulted in nearly 95% of DIRs within tolerance recommendations of TG-132, leading to the majority of features being regarded as acceptably robust. The use of auto contours for radiomic feature analysis is promising but must be done with caution.
A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation
Authors: Wang Li-Ting , Proulx Marie-Ève , Kim Anne D. , Lelarge Virginie , McCaffrey Luke 
Published: November 2021

AbstractApical-basal cell polarity and lumen formation are essential features of many epithelial tissues, which are disrupted in diseases like cancer. Here, we describe a proteomics-based screen to identify proteins involved in lumen formation in three-dimensional spheroid cultures. We established a suspension-based culture method suitable for generating polarized cysts in sufficient quantities for proteomic analysis. Using this approach, we identified several known and unknown proteins proximally associated with PAR6B, an apical protein involved in lumen formation. Functional analyses of candidates identified PARD3B (a homolog of PARD3), RALB, and HRNR as regulators of lumen formation. We also identified PTPN14 as a component of the Par-complex that is required for fidelity of apical-basal polarity. Cells transformed with KRASG12Vexhibit lumen collapse/filling concomitant with disruption of the Par-complex and down-regulation of PTPN14. Enforced expression of PTPN14 maintained the lumen and restricted the transformed phenotype in KRASG12V-expressing cells. This represents an applicable approach to explore protein–protein interactions in three-dimensional culture and to identify proteins important for lumen maintenance in normal and oncogene-expressing cells.
Novel non intrusive continuous use ZeBox technology to trap and kill airborne microbes
Authors: Phadke Kruttika S. , Madival Deepak G. , Venkataraman Janani , Kundu Debosmita , Ramanujan K. S. , Holla Nisha , Arakeri Jaywant , Tomar Gaurav , Datta Santanu , Ghatak Arindam 
Published: November 2021

AbstractPreventing nosocomial infection is a major unmet need of our times. Existing air decontamination technologies suffer from demerits such as toxicity of exposure, species specificity, noxious gas emission, environment-dependent performance and high power consumption. Here, we present a novel technology called “ZeBox” that transcends the conventional limitations and achieves high microbicidal efficiency. In ZeBox, a non-ionizing electric field extracts naturally charged microbes from flowing air and deposits them on engineered microbicidal surfaces. The surface’s three dimensional topography traps the microbes long enough for them to be inactivated. The electric field and chemical surfaces synergistically achieve rapid inactivation of a broad spectrum of microbes. ZeBox achieved near complete kill of airborne microbes in challenge tests (5–9 log reduction) and $$>90\%$$

>
90
%

efficiency in a fully functional stem cell research facility in the presence of humans. Thus, ZeBox fulfills the dire need for a real-time, continuous, safe, trap-and-kill air decontamination technology.
Progestin primed ovarian stimulation using corifollitropin alfa in PCOS women effectively prevents LH surge and reduces injection burden compared to GnRH antagonist protocol
Authors: Huang Ting-Chi , Huang Mei-Zen , Seow Kok-Min , Yang Ih-Jane , Pan Song-Po , Chen Mei-Jou , Hwang Jiann-Loung , Chen Shee-Uan 
Published: November 2021

AbstractUtilizing corifollitropin alfa in GnRH antagonist (GnRHant) protocol in conjunction with GnRH agonist trigger/freeze-all strategy (corifollitropin alfa/GnRHant protocol) was reported to have satisfactory outcomes in women with polycystic ovary syndrome (PCOS). Although lessening in gonadotropin injections, GnRHant were still needed. In addition to using corifollitropin alfa, GnRHant was replaced with an oral progestin as in progestin primed ovarian stimulation (PPOS) to further reduce the injection burden in this study. We try to investigate whether this regimen (corifollitropin alfa/PPOS protocol) could effectively reduce GnRHant injections and prevent premature LH surge in PCOS patients undergoing IVF/ICSI cycles. This is a retrospective cohort study recruiting 333 women with PCOS, with body weight between 50 and 70 kg, undergoing first IVF/ICSI cycle between August 2015 and July 2018. We used corifollitropin alfa/GnRHant protocol prior to Jan 2017 (n = 160), then changed to corifollitropin alfa/PPOS protocol (n = 173). All patients received corifollitropin alfa 100 μg on menstruation day 2/3 (S1). Additional rFSH was administered daily from S8. In corifollitropin alfa/GnRHant group, cetrorelix 0.25 mg/day was administered from S5 till the trigger day. In corifollitropin alfa/PPOS group, dydrogesterone 20 mg/day was given from S1 till the trigger day. GnRH agonist was used to trigger maturation of oocyte. All good quality day 5/6 embryos were frozen, and frozen-thawed embryo transfer (FET) was performed on subsequent cycle. A comparison of clinical outcomes was made between the two protocols. The primary endpoint was the incidence of premature LH surge and none of the patients occurred. Dydrogesterone successfully replace GnRHant to block LH surge while an average of 6.8 days of GnRHant injections were needed in the corifollitropin alfa/GnRHant group. No patients suffered from ovarian hyperstimulation syndrome (OHSS). The other clinical outcomes including additional duration/dose of daily gonadotropin administration, number of oocytes retrieved, and fertilization rate were similar between the two groups. The implantation rate, clinical pregnancy rate, and live birth rate in the first FET cycle were also similar between the two groups. In women with PCOS undergoing IVF/ICSI treatment, corifollitropin alfa/PPOS protocol could minimize the injections burden with comparable outcomes to corifollitropin alfa/GnRHant protocol.
Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
Authors: Shahid Samiah , Shahid Wajeehah , Shaheen Jawaria , Akhtar M. Waheed , Sadaf Saima 
Published: November 2021

AbstractDysregulation of non-coding microRNAs during the course of tumor development, invasion and/or progression to the distant organs, makes them a promising candidate marker for the diagnosis of cancer and associated malignancies. This exploratory study aims at evaluating the usefulness of plasma concentration of circulating mir-146a as a non-invasive biomarker for acute lymphoblastic leukemia (ALL). Total RNA including miRNA was isolated from 110 plasma samples of patients (n = 66), healthy controls (n = 24) and follow up (n = 20) cases and reverse transcribed. Relative concentrations were assessed using real-time quantitative PCR and fold-change was calculated by 2−ΔΔCt method. Finally, relative concentrations were correlated to clinicopathological factors. Patients (n = 66) were analyzed to determine fold expression of miR-146a in plasma samples of ALL. Before chemotherapy, pediatric (n = 42) and adult (n = 24) showed overexpression of miR-146a compared with healthy controls (P < 0.0001). There was no effect of age and gender on mir-146a expression in plasma. mirR-146a expression was independent of clinical and hematological features. Moreover, miR-146a levels in plasma of paired samples (n = 20) after treatment showed significant decrease in expression (P < 0.001). Expression of plasma miR-146a may be utilized as non-invasive marker to diagnose and predict prognosis in pediatric and adult patients with ALL. Moreover predicted targets may be utilized for ALL therapy in future.
A non-optimal cervicovaginal microbiota in pregnancy is associated with a distinct metabolomic signature among non-Hispanic Black individuals
Authors: Gerson Kristin D. , Liao Jingqiu , McCarthy Clare , Burris Heather H. , Korem Tal , Levy Maayan , Ravel Jacques , Elovitz Michal A. 
Published: November 2021

AbstractBiomechanical and molecular processes of premature cervical remodeling preceding spontaneous preterm birth (sPTB) likely result from interactions between the cervicovaginal microbiota and host immune responses. A non-optimal cervicovaginal microbiota confers increased risk of sPTB. The cervicovaginal space is metabolically active in pregancy; microbiota can produce, modify, and degrade metabolites within this ecosystem. We establish that cervicovaginal metabolomic output clusters by microbial community in pregnancy among Black individuals, revealing increased metabolism within the amino acid and dipeptide pathways as hallmarks of a non-optimal microbiota. Few differences were detected in metabolomic profiles when stratified by birth outcome. The study raises the possibility that metabolites could distinguish women with greater risk of sPTB among those with similar cervicovaginal microbiota, and that metabolites within the amino acid and carbohydrate pathways may play a role in this distinction.
Investigation of emission behaviour of perovskite nanocrystals using nano to microspheres of TiO2
Authors: Jha Abha , Shankar Hari , Kar Prasenjit 
Published: November 2021

The encapsulation of MAPbBr3 perovskites nanocrystals into the pores of TiO2 microspheres (m-TiO2) remarkably enhances the stability and PLQY to 95%.
Anisotropic magnetoresistance as evidence of spin-momentum inter-locking in topological Kondo insulator SmB6 nanowires
Authors: Cui Yugui , Chu Yi , Pan Zhencun , Xing Yingjie , Huang Shaoyun , Xu Hongqi 
Published: November 2021

The anisotropic magnetoresistance reveals a signature of spin-momentum inter-locking in surface-states of SmB6 nanowires. Figure (a) in-plane magnetic field magnetoresistance; (b) temperature dependences of the anisotropic magnetoresistance.
2-Pyridinylmethyl borrowing: base-promoted C-alkylation of (pyridin-2-yl)-methyl alcohols with ketones via cleavage of unstrained C(sp3)–C(sp3) bonds
Authors: Hong Chuan-Ming , Zou Fei-Fei , Zhuang Xin , Luo Zhen , Liu Zheng-Qiang , Ren Li-Qing , Li Qing-Hua , Liu Tang-Lin 
Published: November 2021

2-Pyridinylmethyl Borrowing: Transition-metal-free 2-pyridinylmethyl borrowing C-alkylation of alcohols access to ketones is developed. This unstrained C(sp3)–C(sp3) bonds cleavage of unactivated alcohols avoids the use of transition metals.
Do foetal transplant studies continue to be justified in Huntington’s disease?
Authors: Bartley Oliver J.M. , Lelos Mariah J. , Gray William P. , Rosser Anne E. 
Published: November 2021

Abstract
Early CNS transplantation studies used foetal derived cell products to provide a foundation of evidence for functional recovery in preclinical studies and early clinical trials. However, it was soon recognised that the practical limitations of foetal tissue make it unsuitable for widespread clinical use. Considerable effort has since been directed towards producing target cell phenotypes from pluripotent stem cells (PSCs) instead, and there now exist several publications detailing the differentiation and characterisation of PSC-derived products relevant for transplantation in Huntington’s disease (HD). In light of this progress, we ask if foetal tissue transplantation continues to be justified in HD research. We argue that (i) the extent to which accurately differentiated target cells can presently be produced from PSCs is still unclear, currently making them undesirable for studying wider CNS transplantation issues; (ii) foetal derived cells remain a valuable tool in preclinical research for advancing our understanding of which products produce functional striatal grafts and as a reference to further improve PSC-derived products; and (iii) until PSC-derived products are ready for human trials, it is important to continue using foetal cells to gather clinical evidence that transplantation is a viable option in HD and to use this opportunity to optimise practical parameters (such as trial design, clinical practices, and delivery strategies) to pave the way for future PSC-derived products.
Tensor local linear embedding with global subspace projection optimisation
Authors: Niu Guo , Ma Zhengming 
Published: November 2021

AbstractIn this paper, a novel tensor dimensionality reduction (TDR) approach is proposed, which maintains the local geometric structure of tensor data by tensor local linear embedding and explores the global feature by optimising global subspace projection. Firstly, we analyse the local linear feature of tensor data for learning the linear separable embedding of the tenor data. Furthermore, a global subspace projection distance minimisation strategy is introduced to extract the global characteristic of the tensor data. The aim of this strategy is to find an optimal low‐dimensional subspace for TDR. In particular, two novel TDR algorithms are developed by the ensemble of tensor local feature preservation and global subspace projection distance minimisation, which express the subspace projection optimisation as an iteration optimisation problem and a Rayleigh quotient problem, respectively. The extensive experimental results on tensor data classification and clustering have demonstrated the proposed algorithms performed well.
Analysis of wind farm penetration on power swing detection in distance relays
Authors: Niknezhad Maryam , Sadeh Javad 
Published: November 2021

AbstractDistance protection plays an essential role in the protection of sub‐transmission and transmission lines in power systems. However, power swings have occasionally led to the mal‐operation of this type of protection. A significant number of large disturbances, such as blackouts, start with power swings. Thus, improving the circumstances leading to this disruptive phenomenon becomes a great priority. Nowadays, the wind power share of worldwide electricity generation and the use of wind farms have increased. As a result, to predict future events, it is necessary to assess the network in the presence of these renewable sources. In this paper, the effects of the wind resources penetration on power swing have been evaluated. Simulations and analyses have been performed in PSCAD/EMTDC and MATLAB environments, respectively. To provide a comprehensive analysis, maximum power passing through the transmission line and power swing frequency have been utilised as criteria. The results show that wind farms presented acceptable behaviour in the presence of power swing and instability. Therefore, in view point of a power swing, induction generator based wind power plants, compared to synchronous generators, led to better overall conditions. Finally, the effect of fixed and variable speed wind turbines on power swing is compared.