bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics analysis of a human brain sample from a mucolipidosis type IV patient reveals pathophysiological pathways
Authors: Vardi Ayelet , Pri-Or Amir , Wigoda Noa , Grishchuk Yulia , Futerman Anthony H. 
Published: January 2021

AbstractBackgroundMucolipidosis type IV (MLIV), an ultra-rare neurodevelopmental and neurodegenerative disorder, is caused by mutations in theMCOLN1gene, which encodes the late endosomal/lysosomal transient receptor potential channel TRPML1 (mucolipin 1). The precise pathophysiogical pathways that cause neurological disease in MLIV are poorly understood. Recently, the first post-mortem brain sample became available from a single MLIV patient, and in the current study we performed mass spectrometry (MS)-based proteomics on this tissue with a view to delineating pathological pathways, and to compare with previously-published data on MLIV, including studies using theMcoln1−/−mouse.ResultsA number of pathways were altered in two brain regions from the MLIV patient, including those related to the lysosome, lipid metabolism, myelination, cellular trafficking and autophagy, mTOR and calmodulin, the complement system and interferon signaling. Of these, levels of some proteins not known previously to be associated with MLIV were altered, including APOD, PLIN4, ATG and proteins related to interferon signaling. Moreover, when proteins detected by proteomics in the human brain were compared with their orthologs detected in theMcoln1−/−mouse by RNAseq, the results were remarkably similar. Finally, analysis of proteins in human and mouse CSF suggest that calbindin 1 and calbindin 2 might be useful as biomarkers to help chart the course of disease development.ConclusionsDespite the sample size limitations, our findings are consistent with the relatively general changes in lysosomal function previously reported in MLIV, and shed light on new pathways of disease pathophysiology, which is required in order to understand the course of disease development and to determine the efficacy of therapies when they become available for this devastating disease.
What can we learn from common variants associated with unexpected phenotypes in rare genetic diseases?
Authors: Erdmann Jeanette 
Published: January 2021

AbstractThe purpose of this article is to stimulate discussion about whether a phenome-wide association study is a suitable tool for uncovering late-onset risks in patients with monogenic disorders that are not yet fully recognized because the life expectancy of people with such conditions has only recently extended, and they now reach older ages when they may develop additional complications.
Non-SUMOylated CRMP2 decreases NaV1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15
Authors: Gomez Kimberly , Ran Dongzhi , Madura Cynthia L. , Moutal Aubin , Khanna Rajesh 
Published: January 2021

AbstractVoltage-gated sodium channels are key players in neuronal excitability and pain signaling. Functional expression of the voltage-gated sodium channel NaV1.7 is under the control of SUMOylated collapsin response mediator protein 2 (CRMP2). When not SUMOylated, CRMP2 forms a complex with the endocytic proteins Numb, the epidermal growth factor receptor pathway substrate 15 (Eps15), and the E3 ubiquitin ligase Nedd4-2 to promote clathrin-mediated endocytosis of NaV1.7. We recently reported that CRMP2 SUMO-null knock-in (CRMP2K374A/K374A) female mice have reduced NaV1.7 membrane localization and currents in their sensory neurons. Preventing CRMP2 SUMOylation was sufficient to reverse mechanical allodynia in CRMP2K374A/K374A female mice with neuropathic pain. Here we report that inhibiting clathrin assembly in nerve-injured male CRMP2K374A/K374A mice precipitated mechanical allodynia in mice otherwise resistant to developing persistent pain. Furthermore, Numb, Nedd4-2 and Eps15 expression was not modified in basal conditions in the dorsal root ganglia (DRG) of male and female CRMP2K374A/K374A mice. Finally, silencing these proteins in DRG neurons from female CRMP2K374A/K374A mice, restored the loss of sodium currents. Our study shows that the endocytic complex composed of Numb, Nedd4-2 and Eps15, is necessary for non-SUMOylated CRMP2-mediated internalization of sodium channels in vivo.
Involvement of the long noncoding RNA H19 in osteogenic differentiation and bone regeneration
Authors: Zhou Zimo , Hossain Mohammad Showkat , Liu Da 
Published: January 2021

AbstractOsteogenic differentiation and bone regeneration are complex processes involving multiple genes and multiple steps. In this review, we summarize the effects of the long noncoding RNA (lncRNA) H19 on osteogenic differentiation.Osteogenic differentiation includes matrix secretion and calcium mineralization as hallmarks of osteoblast differentiation and the absorption of calcium and phosphorus as hallmarks of osteoclast differentiation. Mesenchymal stem cells (MSCs) form osteoprogenitor cells, pre-osteoblasts, mature osteoblasts, and osteocytes through induction and differentiation. lncRNAs regulate the expression of coding genes and play essential roles in osteogenic differentiation and bone regeneration. The lncRNA H19 is known to have vital roles in osteogenic induction.This review highlights the role of H19 as a novel target for osteogenic differentiation and the promotion of bone regeneration.
Bovine NK-lysin-derived peptides have bactericidal effects against Mycobacterium avium subspecies paratuberculosis
Authors: Dassanayake Rohana P. , Wherry Taylor L. T. , Falkenberg Shollie M. , Reinhardt Timothy A. , Casas Eduardo , Stabel Judith R. 
Published: January 2021

AbstractInfection withMycobacterium aviumsubspeciesparatuberculosis(MAP) is complex, but little is known about the role that natural killer (NK) cells play. In the present study, four bovine NK-lysin peptides were synthesized to evaluate their bactericidal activity against MAP. The results demonstrated that bNK-lysin peptides were directly bactericidal against MAP, with bNK1 and bNK2A being more potent than bNK2B and bNK2C. Mechanistically, transmission electron microscopy revealed that the incubation of MAP with bNK2A resulted in extensive damage to cell membranes and cytosolic content leakage. Furthermore, the addition of bNK2A linked with a cell-penetrating peptide resulted in increased MAP killing in a macrophage model.
Dynamic analysis of a stochastic four species food-chain model with harvesting and distributed delay
Authors: Tuerxun Nafeisha , Teng Zhidong , Chen Wei 
Published: January 2021

AbstractA stochastic four species food-chain model is proposed in this paper. Here, artificial harvest in each species and the effect of time delay for interaction between species are considered, which makes the model more applicable in real situations. Specifically, we address the stochastic global dynamics behavior, including the existence of global positive solutions, stochastic ultimate boundedness, extinction with probability one, persistence in mean and global stability. The asymptotic stability in the probability distribution is obtained, and the criterion for the existence and non-existence of the optimal harvesting strategy is also derived. Furthermore, this paper can provide reference for the research of generaln-species stochastic food-chain models.
Continuous stage stochastic Runge–Kutta methods
Authors: Xin Xuan , Qin Wendi , Ding Xiaohua 
Published: January 2021

AbstractIn this work, a version of continuous stage stochastic Runge–Kutta (CSSRK) methods is developed for stochastic differential equations (SDEs). First, a general order theory of these methods is established by the theory of stochastic B-series and multicolored rooted tree. Then the proposed CSSRK methods are applied to three special kinds of SDEs and the corresponding order conditions are derived. In particular, for the single integrand SDEs and SDEs with additive noise, we construct some specific CSSRK methods of high order. Moreover, it is proved that with the help of different numerical quadrature formulas, CSSRK methods can generate corresponding stochastic Runge–Kutta (SRK) methods which have the same order. Thus, some efficient SRK methods are induced. Finally, some numerical experiments are presented to demonstrate those theoretical results.
Freeze–Thaw Resistance of Ternary Blended Concrete Using Ferronickel Slag
Authors: Cho Won Jung , Kim Min Jae 
Published: January 2021

AbstractThe present study investigated the resistance of concrete blended with ground granulated blast furnace slag (GGBS) and ferronickel slag (FNS) to cycles of freeze and thaw. The replacement ratio of the binders was 0%, 50 wt% of GGBS and 30 wt% of GGBS + 20 wt% of FNS for O100, OG50 and OG30F20, respectively. Specimens consisted of cement paste and concrete kept at 0.45 water/binder ratio. After 28 days of curing, specimens were subjected to freeze and thaw cycles (300) for measuring the variation of strength, weight loss and fundamental transverse frequency. Simultaneously mercury intrusion porosimetry was performed to examine the pore structure modifications at 28 days. The hydration products for cement paste cured at each determined age were characterized by X-ray diffraction and the content of CH and CSH was obtained from thermogravimetric analysis (TGA). As a result, the ternary blended concrete specimens showed lower deterioration degree when subjected to the freeze and thaw cycles. This may be due to a latent hydraulic and/or pozzolanic reaction producing more CSH in the matrix, which in turn increases the volume of small pores. The increased content of C–S–H gel for OG30F20 was confirmed by TGA, accounting for 69.9%. However, the binder system consisting of ordinary Portland cement and GGBS did not exhibit higher resistance to the given deleterious environment, presumably due to a delayed hydration process.
Research progress of heat stroke during 1989–2019: a bibliometric analysis
Authors: Xia De-Meng , Wang Xu-Ren , Zhou Pan-Yu , Ou Tian-Le , Su Lei , Xu Shuo-Gui 
Published: January 2021

Abstract
Background
Heat stroke (HS) is an acute physical disorder that is associated with a high risk of organ dysfunction and even death. HS patients are usually treated symptomatically and conservatively; however, there remains a lack of specific and effective drugs in clinical practice. An analysis of publication contributions from institutions, journals and authors in different countries/regions was used to study research progress and trends regarding HS.

Methods
We extracted all relevant publications on HS between 1989 and 2019 from Web of Science. Using the Statistical Package for Social Science (SPSS, version 24) and the software GraphPad Prism 8, graphs were generated and statistical analyses were performed, while VOSviewer software was employed to visualize the research trends in HS from the perspectives of co-occurring keywords.

Results
As of April 14, 2020, we identified 1443 publications with a citation frequency of 5216. The United States accounted for the largest number of publications (36.2%) and the highest number of citations (14,410), as well as the highest H-index at 74. Although the sum of publications from China ranked second, there was a contradiction between the quantity and quality of publications. Furthermore, Medicine & Science in Sports & Exercise published the most papers related to HS, with Lin MT publishing the most papers in this field (112), while the review by Knochel JP received the highest citation frequency at 969. The keyword heat-stress appeared most recently, with an average appearing year of 2015.5. In the clinical research cluster, exertional heat-stroke was determined to be the hotspot, while ambient-temperature and heat waves were the new trends in the epidemiological research cluster.

Conclusions
Corresponding to this important field, while the contributions of the publications from the United States were significant, the mismatch between the quantity and quality of publications from China must be examined. Moreover, it is hypothesized that clinical and epidemiological studies may become hotspots in the near future.
Multivariate prediction of mixed, multilevel, sequential outcomes arising from in vitro fertilisation
Authors: Wilkinson Jack , Vail Andy , Roberts Stephen A. 
Published: January 2021

AbstractIn vitro fertilisation (IVF) comprises a sequence of interventions concerned with the creation and culture of embryos which are then transferred to the patient’s uterus. While the clinically important endpoint is birth, the responses to each stage of treatment contain additional information about the reasons for success or failure. As such, the ability to predict not only the overall outcome of the cycle, but also the stage-specific responses, can be useful. This could be done by developing separate models for each response variable, but recent work has suggested that it may be advantageous to use a multivariate approach to model all outcomes simultaneously. Here, joint analysis of the sequential responses is complicated by mixed outcome types defined at two levels (patient and embryo). A further consideration is whether and how to incorporate information about the response at each stage in models for subsequent stages. We develop a case study using routinely collected data from a large reproductive medicine unit in order to investigate the feasibility and potential utility of multivariate prediction in IVF. We consider two possible scenarios. In the first, stage-specific responses are to be predicted prior to treatment commencement. In the second, responses are predicted dynamically, using the outcomes of previous stages as predictors. In both scenarios, we fail to observe benefits of joint modelling approaches compared to fitting separate regression models for each response variable.
The German RECAP questionnaire: linguistic validation and cognitive debriefing in German adults with self-reported atopic eczema and parents of affected children
Authors: Gabes Michaela , Tischer Christina , Herrmann Anne , Howells Laura , Apfelbacher Christian 
Published: January 2021

Abstract
Background
Recap of atopic eczema (RECAP) is a patient-reported outcome measure (PROM) assessing eczema control. Long-term control of eczema is one of the four core outcome domains for atopic eczema trials. This instrument has been recently developed in the UK.

Objective
This study aimed to translate the English RECAP into German and test its content validity in a German population with self-reported atopic eczema.

Methods
A six-step procedure including two forward and one backward translations, two consensus decisions and an expert review was performed to obtain a German version of RECAP. We conducted semi-standardized cognitive interviews with adults with atopic eczema (n = 7) and parents having children affected by this disease (n = 5). A “think-aloud” method was used and aspects of comprehensibility, comprehensiveness and relevance according to the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) criteria were examined. Interviews were coded using qualitative content analysis.

Results
No particular linguistic problems were encountered during forward-backward translation. Minor wording changes were made as required. The title was adjusted to a more familiar German term of the disease (which is ‘Neurodermitis’). The recall period was rephrased from ‘over the last week’ to ‘over the last seven days’ since there was a different cultural understanding of the time frame. Regarding content validity, the items of the German RECAP were considered to be comprehensible, comprehensive and relevant for the participants and parents of affected children. The participants understood the instruction and considered the one-week recall period and the response options as appropriate.

Conclusions
A German version of RECAP that is linguistically equivalent to the original version is now available but further assessment of its measurement properties is needed.
Assessment of seasonal variation on neonatal sepsis
Authors: Abdel Mawla Mohamed A. , Mostafa Eman A. , Hasanin Rasha M. , Salah Mohab M. 
Published: January 2021

AbstractBackgroundNeonatal sepsis is the major cause of morbidity and mortality in neonates. Frequency of bacterial sepsis is affected by seasonal variations. This study was done to rule out the effects of seasonal variation on neonatal sepsis and to determine isolated bacterial agents and their antibiotic sensitivity profile.ResultsThis cross-sectional study included100 neonates with suspected sepsis, and conducted during summer and winter seasons. High frequencies of neonatal morbidities were observed like poor feeding (98%), Jaundice (82%), pallor (76%), respiratory distress (66%) and convulsions (64%) in winter, whereas fever (40%) and edema (12%) in summer. Statically significant correlation was found between the seasonal variations and different symptomatic expression of bacteremia like jaundice, respiratory distress, convulsions and poor feeding (pvalue < 0.001) in winter, while pallor, fever and edema (pvalue < 0.05) in summer. Our study showed high proportion of neonatal infection with Gram negative bacteria, as (40%)Pseudomonas aeruginosaand (16%)Escherichia coliin winter season, while (22%) Enterobacter in summer. Carbapenems was very effective againstPseudomonas aeruginosaisolates,Klebsiella pneumonia, Enterobacter, Acinetobacter,Escherichia coli,Staphylococcus aureusand Listeria, while theses bacterial isolates showed (80–100%) resistance to Unasyn and Cephalosporins (3rd generation).ConclusionThis study showed that Seasonal variations had effects on neonatal sepsis regarding their both different symptomatic expressions and bacterial isolates. Most of isolates were sensitive to Carbapenems and resistant to both Unasyn and Cephalosporins (3rd generation).
Do competition and efficiency lead to bank stability? Evidence from Bangladesh
Authors: Dutta Kumar Debasis , Saha Mallika 
Published: January 2021

AbstractFinancial deregulation after financial repression during 1980s and 1990s has stimulated a fierce competition among banks across the world. In pace with this, banking industry of Bangladesh is also experiencing an intense competition, since it is composed of a large number of banks. Considering this upsurge, our study aims to explore the impact of competition and efficiency on financial stability of Bangladeshi banks over 2009–2017. For exploring this nexus, we calculate Boone indicator and Z-score, construct banking efficiency index by principal component analysis, using bank-level data to measure competition, stability and efficiency, respectively, and analyze the impact of efficiency on financial stability at different levels of competition. We address the endogeneity of the estimation by employing two-step system GMM and different robustness checks. The findings of our study suggest a nonlinear competition–stability relationship, and though efficiency contributes to stability, the impact is moderated in the presence of competition. Our findings are robust to alternative measures of competition, stability and control variables, which could be useful for policy makers to formulate strategies and policies to maintain financial stability.
Iron-Catalyzed Cross-Coupling Reactions Tuned by Bulky Ortho-Phenylene Bisphosphine Ligands
Authors: Adak Laksmikanta , Hatakeyama Takuji , Nakamura Masaharu 
Published: January 2021

Abstract
The significant progress made in the context of iron-catalyzed cross-coupling reactions in the past several years has boosted competition with well-known nickel and palladium catalyst systems. Iron-catalyzed protocols not only benefit from economic and toxicology standpoints, but also exhibit a range of reactivities and tolerate many functional groups. To date several iron catalyst systems have been developed, ranging from the use of simple iron salts to more complex stoichiometric/catalytic modifiers. Effective N-heterocyclic carbenes and bisphosphine ligands have also been developed for the cross-coupling reactions of organic electrophiles using diverse organometallic reagents. Indeed, the use of bisphosphine ligands in the field of iron-catalyzed cross-coupling reactions is important for new applications in modern synthetic organic chemistry. This account summarizes the recent developments in practical and novel iron-catalyzed cross-coupling reactions employing bulky o-phenylene bisphosphine ligands and their mechanistic views.
Preparation of Palladium Metal Intercalated between Graphite Layers with Low Palladium Loading for Cinnamaldehyde Hydrogenation
Authors: Sodeno Mika , Nanao Hidetaka , Shirai Masayuki 
Published: January 2021

Abstract
Spherical palladium nanoparticles between graphite layers were prepared by the intercalation of palladium chloride followed by its reduction between graphite layers. The palladium particles intercalated between graphite layers were more active for cinnamaldehyde hydrogenation to hydrocinnamaldehyde and hydrocinnamyl alcohol in n-heptane solvent than those supported on graphite surface.
Effects of Different Exercise Strategies to Improve Postprandial Glycemia in Healthy Individuals
Authors: BELLINI ALESSIO , NICOLÒ ANDREA , BAZZUCCHI ILENIA , SACCHETTI MASSIMO 
Published: January 2021

ABSTRACT

Purpose
We systematically investigated the effects of different exercise strategies on postprandial glycemia.

Methods
Six randomized repeated-measures crossover studies were performed. Study 1 compared the effects of 60 min of brisk walking started at 30, 60, or 90 min after breakfast on postbreakfast and postlunch glycemic responses. Study 2 investigated the effects of 30 min of different exercise types (aerobic vs resistance vs combined). Study 3 compared the effects of 30 min of different aerobic exercise types (walking vs cycling vs elliptical). Study 4 evaluated the effects of 30 min of brisk walking performed 45 min before or 15 and 30 min after breakfast. Study 5 compared 30 with 45 min of postprandial brisk walking. Study 6 compared the effects of a total of 30 min brisk walking exercise fragmented in bouts of 15, 5, or 2.5 min performed every 15 min.

Results
Postprandial but not preprandial exercise improved glycemic response (studies 1 and 4). The glycemic peak was attenuated only when exercise started 15 min after the meal (study 4). A similar reduction of the postprandial glycemic response was observed with different exercise types (studies 2 and 3). Thirty and 45 min of brisk walking provided a similar reduction of the postprandial glucose response (study 5). When performing activity breaks, 10 and 20 min of cumulative exercise were sufficient to attenuate postprandial glycemia in the first hour postmeal (study 6).

Conclusion
Our findings provide insight into how to choose timing, type, duration, and modality for postprandial exercise prescription in healthy individuals.