bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphorylation of transcription factor bZIP21 by MAP kinase MPK6-3 enhances banana fruit ripening
Authors: Wu Chao-Jie , Shan Wei , Liu Xun-Cheng , Zhu Li-Sha , Wei Wei , Yang Ying-Ying , Guo Yu-Fan , Bouzayen Mondher , Chen Jian-Ye , Lu Wang-Jin , Kuang Jian-Fei 
Published: November 2021

Abstract
Ripening of fleshy fruits involves both diverse post-translational modifications (PTMs) and dynamic transcriptional reprogramming, but the interconnection between PTMs, such as protein phosphorylation and transcriptional regulation, in fruit ripening remains to be deciphered. Here, we conducted a phosphoproteomic analysis during banana (Musa acuminata) ripening and identified 63 unique phosphopeptides corresponding to 49 proteins. Among them, a Musa acuminata basic leucine zipper transcription factor21 (MabZIP21) displayed elevated phosphorylation level in the ripening stage. MabZIP21 transcript and phosphorylation abundance increased during banana ripening. Genome-wide MabZIP21 DNA binding assays revealed MabZIP21-regulated functional genes contributing to banana ripening, and electrophoretic mobility shift assay, chromatin immunoprecipitation coupled with quantitative polymerase chain reaction, and dual-luciferase reporter analyses demonstrated that MabZIP21 stimulates the transcription of a subset of ripening-related genes via directly binding to their promoters. Moreover, MabZIP21 can be phosphorylated by MaMPK6-3, which plays a role in banana ripening, and T318 and S436 are important phosphorylation sites. Protein phosphorylation enhanced MabZIP21-mediated transcriptional activation ability, and transient overexpression of the phosphomimetic form of MabZIP21 accelerated banana fruit ripening. Additionally, MabZIP21 enlarges its role in transcriptional regulation by activating the transcription of both MaMPK6-3 and itself. Taken together, this study reveals an important machinery of protein phosphorylation in banana fruit ripening in which MabZIP21 is a component of the complex phosphorylation pathway linking the upstream signal mediated by MaMPK6-3 with transcriptional controlling of a subset of ripening-associated genes.
Torque Teno Virus quantification for monitoring of immunomodulation with biologic compounds in the treatment of rheumatoid arthritis
Authors: Studenic Paul , Bond Gregor , Kerschbaumer Andreas , Bécède Manuel , Pavelka Karel , Karateev Dmitry , Stieger Jutta , Puchner Rudolf , Mueller Ruediger B , Puchhammer-Stöckl Elisabeth  , Durechova Martina , Loiskandl Michaela , Perkmann Thomas  , Olejarova Martina , Luchikhina Elena , Steiner Carl-Walter , Bonelli Michael  , Smolen Josef S , Aletaha Daniel 
Published: November 2021

Abstract

Objectives
RA patients who fail to respond to MTX can receive biologic dMARDs (bDMARDs). The Torque Teno Virus (TTV) is a potential novel candidate for monitoring of immunosuppression. We explore TTV in these patients and its association with clinical response to bDMARDs.

Methods
The BioBio Study is a multicentre randomized open-label trial, including RA patients with insufficient response to MTX. Patients were randomized to either TNFi (infliximab, INF), anti-IL-6 (tocilizumab, TCZ), CTLA4-Ig (abatacept, ABA) or anti-CD20 (rituximab, RTX) in addition to MTX. PCR was used to quantify TTV in the peripheral blood.

Results
TTV was measured in 95 patients (INF, n = 23; TCZ, n = 22; ABA, n = 27; RTX; n = 23). TTV increased by a median of 4.5 × 104 copies/ml [c/ml; interquartile range (IQR) 0–7.5 × 105] after 3 months. TTV levels at month 3 were associated with the Simplified Disease Activity Index (SDAI) (P = 0.03) and the Clinical Disease Activity Index (CDAI) response (P = 0.026) at month 6. A TTV cut-off level of 1.2 × 106 c/ml at month 3 had a positive likelihood ratio of 2.7 for prediction of an 85% reduction in SDAI at month 6.

Conclusion
Our data suggest that TTV levels increase upon TNF, CD20 and costimulation blockade and are associated with the clinical response to bDMARDs in RA patients.

Trial registration
ClinicalTrials.gov; https://clinicaltrials.gov; NCT01638715
Work participation in patients with systematic lupus erythematosus: a systematic review
Authors: Blomjous Birgit S , Gajadin Gayle R S , Voskuyl Alexandre E , Falzon Louise , Hoving Jan L , Bultink Irene E M , ter Wee Marieke M 
Published: November 2021

Abstract

Objectives
This systematic review assessed which variables are associated with or are predictors for work participation outcomes in patients with systematic lupus erythematosus (SLE).

Methods
A literature search using MEDLINE, The Cochrane Library, Embase and CINAHL was conducted to identify all studies published from inception (1947) to June 2021 on factors related to and/or predicting employment status, absenteeism and/or presenteeism in SLE patients aged ≥18 years. The quality of included articles was assessed using the QUIPS tool. Narrative summaries were used to present the data.

Results
Fifteen studies (nine on associations, four on predictions, and two assessing both) were included, encompassing data of 3800 employed patients. Younger age, Caucasian ethnicity, higher educational level, lower disease activity score, shorter disease duration, absence of specific disease manifestations, higher levels of physical functioning and less physical job demands and higher levels of psychological/cognitive functioning were associated with or predicted favorable work outcomes. Older age, non-Caucasian ethnicity, female gender, never being married, poverty, lower educational level, higher disease activity score, longer disease duration, specific disease manifestations, lower levels of physical functioning, more physical job demands and low job control, less job tenure and lower levels of cognitive functioning were associated with or predicted an unfavorable work outcome. Limitations of the evidence were the quality of the studies and the use of heterogeneous outcome measures, applied statistical methods and instruments used to assess work participation.

Conclusion
We recommend applying the EULAR points to consider for designing, analysing and reporting on work participation in inflammatory arthritis also to SLE studies on work participation, to enhance the quality and comparability between studies and to better understand the impact of SLE on work participation.

Trial registration
registration in PROSPERO (CRD42020161275; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=161275).
The Mechanosensitive Ion Channel MSL10 Modulates Susceptibility to Pseudomonas syringae in Arabidopsis thaliana
Authors: Basu Debarati , Codjoe Jennette M. , Veley Kira M. , Haswell Elizabeth S. 
Published: November 2021

Plants sense and respond to molecular signals associated with the presence of pathogens and their virulence factors. Mechanical signals generated during pathogenic invasion may also be important, but their contributions have rarely been studied. Here, we investigate the potential role of a mechanosensitive ion channel, MscS-like (MSL)10, in defense against the bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. We previously showed that overexpression of MSL10-GFP, phospho-mimetic versions of MSL10, and the gain-of-function allele msl10-3G all produce dwarfing, spontaneous cell death, and the hyperaccumulation of reactive oxygen species. These phenotypes are shared by many autoimmune mutants and are frequently suppressed by growth at high temperature in those lines. We found that the same was true for all three MSL10 hypermorphs. In addition, we show that the SGT1/RAR1/HSP90 cochaperone complex was required for dwarfing and ectopic cell death, PAD4 and SID2 were partially required, and the immune regulators EDS1 and NDR1 were dispensable. All MSL10 hypermorphs exhibited reduced susceptibility to infection by P. syringae strain Pto DC3000 and Pto DC3000 expressing the avirulence genes avrRpt2 or avrRpm1 but not Pto DC3000 hrpL and showed an accelerated induction of PR1 expression compared with wild-type plants. Null msl10-1 mutants were delayed in PR1 induction and displayed modest susceptibility to infection by coronatine-deficient P. syringae pv. tomato. Finally, stomatal closure was reduced in msl10-1 loss-of-function mutants in response to P. syringae pv. tomato COR−. These data show that MSL10 modulates pathogen responses and begin to address the possibility that mechanical signals are exploited by the plant for pathogen perception. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Pain and Opioid Consumption and Mobilization after Surgery: Post Hoc Analysis of Two Randomized Trials
Authors: Rivas Eva , Cohen Barak , Pu Xuan , Xiang Li , Saasouh Wael , Mao Guangmei , Minko Paul , Mosteller Lauretta , Volio Andrew , Maheshwari Kamal , Sessler Daniel I. , Turan Alparslan 
Published: November 2021

Background
Early mobilization is incorporated into many enhanced recovery pathways. Inadequate analgesia or excessive opioids may restrict postoperative mobilization. The authors tested the hypotheses that in adults recovering from abdominal surgery, postoperative pain and opioid consumption are inversely related to postoperative mobilization, and that postoperative mobilization is associated with fewer potentially related complications.

Methods
The authors conducted a subanalysis of two trials that enrolled adults recovering from abdominal surgery. Posture and movement were continuously monitored for 48 postoperative hours using noninvasive untethered monitors. Mobilization was defined as the fraction of monitored time spent sitting or standing.

Results
A total of 673 patients spent a median [interquartile range] of 7% [3 to 13%] of monitored time sitting or standing. Mobilization time was 1.9 [1.0 to 3.6] h/day for patients with average pain scores 3 or lower, but only 1.2 [0.5 to 2.6] h/day in those with average scores 6 or greater. Each unit increase in average pain score was associated with a decrease in mobilization time of 0.12 (97.5% CI, 0.02 to 0.24; P = 0.009) h/day. In contrast, there was no association between postoperative opioid consumption and mobilization time. The incidence of the composite of postoperative complications was 6.0% (10 of 168) in the lower mobilization quartile, 4.2% (7 of 168) in the second quartile, and 0% among 337 patients in the highest two quartiles (P = 0.009).

Conclusions
Patients recovering from abdominal surgery spent only 7% of their time mobilized, which is considerably less than recommended. Lower pain scores are associated with increased mobility, independently of opioid consumption. Complications were more common in patients who mobilized poorly.

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What This Article Tells Us That Is New
Vocal communication across cultures: theoretical and methodological issues
Authors: Bryant Gregory A. 
Published: November 2021

The study of human vocal communication has been conducted primarily in Western, educated, industrialized, rich, democratic (WEIRD) societies. Recently, cross-cultural investigations in several domains of voice research have been expanding into more diverse populations. Theoretically, it is important to understand how universals and cultural variations interact in vocal production and perception, but cross-cultural voice research presents many methodological challenges. Experimental methods typically used in WEIRD societies are often not possible to implement in many populations such as rural, small-scale societies. Moreover, theoretical and methodological issues are often unnecessarily intertwined. Here, I focus on three areas of cross-cultural voice modulation research: (i) vocal signalling of formidability and dominance, (ii) vocal emotions, and (iii) production and perception of infant-directed speech. Research in these specific areas illustrates challenges that apply more generally across the human behavioural sciences but also reveals promise as we develop our understanding of the evolution of human communication.This article is part of the theme issue ‘Voice modulation: from origin and mechanism to social impact (Part II)’.
Musicality in human vocal communication: an evolutionary perspective
Authors: Leongómez Juan David , Havlíček Jan , Roberts S. Craig 
Published: November 2021

Studies show that specific vocal modulations, akin to those of infant-directed speech (IDS) and perhaps music, play a role in communicating intentions and mental states during human social interaction. Based on this, we propose a model for the evolution of musicality—the capacity to process musical information—in relation to human vocal communication. We suggest that a complex social environment, with strong social bonds, promoted the appearance of musicality-related abilities. These social bonds were not limited to those between offspring and mothers or other carers, although these may have been especially influential in view of altriciality of human infants. The model can be further tested in other species by comparing levels of sociality and complexity of vocal communication. By integrating several theories, our model presents a radically different view of musicality, not limited to specifically musical scenarios, but one in which this capacity originally evolved to aid parent–infant communication and bonding, and even today plays a role not only in music but also in IDS, as well as in some adult-directed speech contexts.This article is part of the theme issue ‘Voice modulation: from origin and mechanism to social impact (Part II)’.
A cross-species framework to identify vocal learning abilities in mammals
Authors: Ravignani Andrea , Garcia Maxime 
Published: November 2021

Vocal production learning (VPL) is the experience-driven ability to produce novel vocal signals through imitation or modification of existing vocalizations. A parallel strand of research investigates acoustic allometry, namely how information about body size is conveyed by acoustic signals. Recently, we proposed that deviation from acoustic allometry principles as a result of sexual selection may have been an intermediate step towards the evolution of vocal learning abilities in mammals. Adopting a more hypothesis-neutral stance, here we perform phylogenetic regressions and other analyses further testing a potential link between VPL and being an allometric outlier. We find that multiple species belonging to VPL clades deviate from allometric scaling but in the opposite direction to that expected from size exaggeration mechanisms. In other words, our correlational approach finds an association between VPL and being an allometric outlier. However, the direction of this association, contra our original hypothesis, may indicate that VPL did not necessarily emerge via sexual selection for size exaggeration: VPL clades show higher vocalization frequencies than expected. In addition, our approach allows us to identify species with potential for VPL abilities: we hypothesize that those outliers from acoustic allometry lying above the regression line may be VPL species. Our results may help better understand the cross-species diversity, variability and aetiology of VPL, which among other things is a key underpinning of speech in our species.
This article is part of the theme issue ‘Voice modulation: from origin and mechanism to social impact (Part II)’.
The neural control of volitional vocal production—from speech to identity, from social meaning to song
Authors: Scott Sophie K. 
Published: November 2021

The networks of cortical and subcortical fields that contribute to speech production have benefitted from many years of detailed study, and have been used as a framework for human volitional vocal production more generally. In this article, I will argue that we need to consider speech production as an expression of the human voice in a more general sense. I will also argue that the neural control of the voice can and should be considered to be a flexible system, into which more right hemispheric networks are differentially recruited, based on the factors that are modulating vocal production. I will explore how this flexible network is recruited to express aspects of non-verbal information in the voice, such as identity and social traits. Finally, I will argue that we need to widen out the kinds of vocal behaviours that we explore, if we want to understand the neural underpinnings of the true range of sound-making capabilities of the human voice.
This article is part of the theme issue ‘Voice modulation: from origin and mechanism to social impact (Part II)’.
Vocal size exaggeration may have contributed to the origins of vocalic complexity
Authors: Pisanski Katarzyna , Anikin Andrey , Reby David 
Published: November 2021

Vocal tract elongation, which uniformly lowers vocal tract resonances (formant frequencies) in animal vocalizations, has evolved independently in several vertebrate groups as a means for vocalizers to exaggerate their apparent body size. Here, we propose that smaller speech-like articulatory movements that alter only individual formants can serve a similar yet less energetically costly size-exaggerating function. To test this, we examine whether uneven formant spacing alters the perceived body size of vocalizers in synthesized human vowels and animal calls. Among six synthetic vowel patterns, those characterized by the lowest first and second formant (the vowel /u/ as in ‘boot’) are consistently perceived as produced by the largest vocalizer. Crucially, lowering only one or two formants in animal-like calls also conveys the impression of a larger body size, and lowering the second and third formants simultaneously exaggerates perceived size to a similar extent as rescaling all formants. As the articulatory movements required for individual formant shifts are minor compared to full vocal tract extension, they represent a rapid and energetically efficient mechanism for acoustic size exaggeration. We suggest that, by favouring the evolution of uneven formant patterns in vocal communication, this deceptive strategy may have contributed to the origins of the phonemic diversification required for articulated speech.
This article is part of the theme issue ‘Voice modulation: from origin and mechanism to social impact (Part II)’.
AI/ML Models to Aid in the Diagnosis of COVID-19 Illness from Forced Cough Vocalizations: Results and Challenges of a Systematic Review of the Relevant Literature
Authors: Kelley K. , Sakara A.A. , Kelley M. , Kelley S. C. , McLenaghan P. , Aldir R. , Cox M. , Donaldson N. , Stogsdill A. , Kotchou S. , Sula G. , Ramirez M.A. 
Published: November 2021

AbstractFrom a comprehensive and systematic search of the relevant literature on signal data signature (SDS)-based artificial intelligence/machine learning (AI/ML) systems designed to aid in the diagnosis of COVID-19 illness, we aimed to reproduce the reported systems and to derive a performance goal for comparison to our own medical device with the same intended use. These objectives were in line with a pathway to regulatory approval of such devices, as well as to acceptance of this unfamiliar technology by disaster/pandemic decision makers and clinicians. To our surprise, none of the peer-reviewed articles or pre-print server records contained details sufficient to meet the planned objectives. Information amassed from the full review of more than 60 publications, however, did underscore discrete impediments to bringing AI/ML diagnostic solutions to the bedside during a pandemic. These challenges then were explored by the authors via a gap analysis and specific remedies were proposed for bringing AI/ML technologies in closer alignment with the needs of a Total Product Life Cycle (TPLC) regulatory approach.
Functional ultrasound imaging of recent and remote memory recall in the associative fear neural network in mice
Authors: Grohs-Metz Gillian , Smausz Rebecca , Gigg John , Boeckers Tobias , Hengerer Bastian 
Published: November 2021

Scientific AbstractEmotional learning and memory are affected in numerous psychiatric disorders. At a systems level, however, the underlying neural circuitry is not well defined. Rodent fear conditioning (FC) provides a translational model to study the networks underlying associative memory retrieval. In the current study, functional connectivity among regions related to the cue associative fear network were investigated using functional ultrasound (fUS), a novel imaging technique with great potential for detecting regional neural activity through cerebral blood flow. Behavioral fear expression and fUS imaging were performed one and thirty-one days after FC to assess recent and remote memory recall. Cue-evoked increases in functional connectivity were detected throughout the amygdala, with the lateral (LA) and central (CeA) amygdalar nuclei emerging as major hubs of connectivity, though CeA connectivity was reduced during remote recall. The hippocampus and sensory cortical regions displayed heightened connectivity with the LA during remote recall, whereas interconnectivity between the primary auditory cortex and temporal association areas was reduced. Subregions of the prefrontal cortex exhibited variable connectivity changes, where prelimbic connectivity with the amygdala was refined while specific connections between the infralimbic cortex and amygdalar subregions emerged during remote memory retrieval. Moreover, freezing behavior positively correlated with functional connectivity between hubs of the associative fear network, suggesting that emotional response intensity reflected the strength of the cue-evoked functional network. Overall, our data provide evidence of the functionality of fUS imaging to investigate the neural dynamics of memory encoding and retrieval, applicable in the development of innovative treatments for affective disorders.HighlightsFunctional ultrasound imaging can elucidate fear associated neural networksFreezing behavior correlates with cue-evoked functional connectivity changesThe lateral and central amygdalar nuclei are major hubs in the fear networkThe hippocampus is active during recent and remote cued fear memory retrievalConnectivity profiles of the prelimbic and infralimbic areas vary in remote recall