bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosensor-enabled on-site therapeutic drug monitoring of antibiotics
Authors: Dincer Can , Ates Hatice Ceren , Mohsenin Hasti , Wenzel Christin , Glatz Regina , Wagner Hanna , Bruch Richard , Höfflin Nico , Spassov Sashko , Streicher Lea , Lozano-Zahonero Sara , Flamm Bernd , Köhn Maja , Schmidt Johannes , Schumann Stefan , Urban Gerald , Weber Wilfried 
Published: May 2021

Abstract
Antimicrobial resistance is increasing with an alarming rate for which the prime suspect is the “one size-fits-all” dosage strategies of antibiotics. Personalized antibiotherapy framework appears as a viable option to counteract inadequate dosage, as it offers the application of the optimal dosage regimen for each individual. Such individualized scheme, however, needs frequent sampling to tailor the blood antibiotic concentration to respond unique pharmacokinetic/pharmacodynamic (PK/PD) of the patient. Herein, there are two alternative paths for feasible therapeutic drug monitoring (TDM); transforming our understanding to utilize blood based sampling within the scope of point-of-care (POC), or focusing on non-invasive samples. Here, we present a versatile biosensor along with an antibody-free assay that can be utilized in both paths for on-site TDM. The developed platform is evaluated in a large animal study (pigs exposed with overdose, normal dose, and underdose of ß lactams), in which antibiotic concentrations are quantified in matrices including whole blood, plasma, urine, saliva, and exhaled breath condensate (EBC). Herein, the detection and the clearance of drug concentrations in EBC is demonstrated for the first time. Influence of the secretion mechanisms on measured drug concentrations is then quantified by comparing the plasma concentrations with those in EBC, saliva and urine. The potential of the developed platform for blood-based POC application is further illustrated by tracking ß lactam concentrations in untreated blood samples. Finally, multiplexing capabilities are explored successfully for multianalyte/sample analysis. Enabling a rapid, low-cost, sample-independent, and multiplexed on-site TDM, this system could pave the way for the personalized drug therapies and thus, shift the paradigm of “one size-fits-all” strategies.
Phenol Removal using Corona Discharge Plasma Combined with Porous Polyaniline Nanofiber
Authors: Khalil Ahmed El-Tayeb , Noby Hussain , ELSHAZLY AHMED H , Elkady Marwa F. 
Published: May 2021

Abstract
A contemporary design for a recirculated flow dual remediation system was successfully developed for phenol remediation. The system involved Corona Discharge Plasma (CDP), accompanied by Polyaniline Nanofiber (PANNFs) as a solid adsorbent. PANNFs was obtained using simple chemical oxidation polymerization at room temperature. Different chemo-physical characterization techniques were employed to examine the produced polyaniline such as Fourier Transform Infrared spectroscopy (FT-IR), X-ray Diffraction (XRD), and Brunauer, Emmett, and Teller (BET) surface area analysis. The primary purpose of the used PANNF powder is to facilitate the phenol degradation using plasma by collecting the phenol molecules on the surface of PANNFs. The phenol removal percentage of 99% was attained at a treatment time of 60 min using the developed dual system. Finally, a slight synergetic effect between the used two remediation processes, PANI as adsorbent and CDP treatment, was approved. PANNFs existence in the remediation system also helps to save the consumed power in degradation using CDP.
Altering Dynamics of Autonomic Processing Therapy (ADAPT) Trial: A Novel, Targeted Treatment for Reducing Anxiety in Joint Hypermobility
Authors: Davies Geoff , Csecs Jenny LL , Ball Heather , Dare Jess , Hosking Robin , Critchley Hugo D , Grey Nick , Eccles Jessica A 
Published: May 2021

Abstract
Background: Hypermobility is a poorly-recognised and understood musculoskeletal disorder thought to affect around 20% of the population. Hypermobility is associated with reduced physiological and psychological functioning, quality of life, and is a known risk factor for the development of an anxiety disorder. To date no evidence-based, targeted treatment for anxiety in the context of hypermobility exists. The present intervention (ADAPT) is a novel therapy combining cognitive approaches from clinical health psychology targeting the catastrophisation of internal sensations, with bio-behavioural training improving autonomic trait prediction error.Method: Eighty individuals with diagnosed hypermobility will be recruited and the efficacy of ADAPT to treat anxiety will be compared to an Emotion-Focused Supportive Therapy (EFST) comparator therapy in a randomised, controlled trial. The primary treatment target will be post therapy score on the Beck Anxiety Inventory and secondary outcomes will also be considered in relation to anxiety, depression, alexithymia, social and work adjustment, panic symptoms and dissociation. Due to COVID restrictions, the intervention will be moved to online delivery and qualitative assessment of treatment tolerance to online therapy will also be assessed.Discussion: Online delivery of an intervention targeting anxiety would improve the quality of life for those experiencing anxiety disorder, and help the £11.7 billion that anxiety disorders cost the UK economy annually.WHO trial registration serial number 40233 registered 20/2/2019 available at https://doi.org/10.1186/ISRCTN17018615
Comprehensive analysis of EMT-related genes and lncRNAs in the prognosis, immunity, and drug treatment of colorectal cancer
Authors: Yang Yang , Feng Mingyang , Bai LiangLiang , Liao Weiting , Zhou Kexun , Zhang Mengxi , Wu Qiuji , Wen Feng , Lei Wanting , Zhang Pengfei , Zhang Nan , Huang Jiaxing , Li Qiu 
Published: May 2021

Abstract
Background: EMT is an important biological process in the mechanism of tumor metastasis. However, there are still many unknowns about the specific mechanism of EMT. At present, a comprehensive analysis of EMT-related genes in colorectal cancer (CRC) is still lacking. Methods: All the data were downloaded to public databases including TCGA as a training set and GEO as a validation set. Bioinformatics methods were used to analyze the correlation between EMT-related genes (EMT-RDGs) and CRC prognosis, metastasis, drug efficacy and immunity.Results: We finally obtained 9 prognostic-related EMT-RDGs (FGF8, NOG, PHLDB2, SIX2, SNAI1, TBX5, TIAM1, TWIST1, TCF15) through differential expression analysis, Unicox and Lasso regression analysis, and then constructed a risk prognosis model. There were significant differences in clinical characteristics, 22 immune cells and immune functions between the high and low-risk groups and the different states of the 9 prognostic-related EMT-RDGs. The methylation level and mutation status of 9 prognostic-related EMT-RDGs all affect their regulation of EMT. The Cox proportional hazards regression model was also constructed by the methylation sites of 9 prognostic-related EMT-RDGs. In addition, The expression of FGF8, PHLDB2, SIX2 and SNAIL was higher and the expression level of NOG and TWIST1 was lower in the non-metastasis CRC group. 9 prognostic-related EMT-RDGs also affected the drug treatment response of CRC. Conclusions: Targeting these 9 prognostic-related EMT-RDGs can regulate CRC metastasis and immune, which is beneficial to the prognosis of CRC patients, improve drug sensitivity in CRC patients.
Investigation of the Interaction, Rheological and Tribological Properties of Bis(pinacolato)diboron with Lithium Grease During Mechanical Aging
Authors: Ren Jia , Gong Kuiliang , Zhao Gaiqing , Wu Xinhu , Wang Xiaobo 
Published: May 2021

Abstract
In this article, Lewis acid–base complex of lithium 12-hydroxystearate (LHS) with diboron compound is formed by the introduction of bis(pinacolato)diboron (B2Pin2) into lithium grease. The interaction between Lewis acid B2Pin2 and Lewis base RCO2− of LHS is characterized by various techniques. Moreover, the rheological and tribological behaviors of the base grease are evaluated at low and moderate temperature, and the results indicate that the addition of B2Pin2 noticeably enhanced the rheological, friction-reducing, and anti-wear (AW) properties of the base grease, which likely owing to the fact that the formation of Lewis acid–base complex is beneficial for improving the soap fiber structure strength, helping to prevent the mechanical degradation of the lithium grease during mechanical aging process.
The correlation between single nucleotide polymorphisms of the thymic stromal lymphopoietin receptor and breast cancer in a cohort of female patients in Saudi Arabia
Authors: Semlali ِAbdelhabib , Almutairi Mikhlid H. , Alharbi Sultan N. , Alamri Abdullah M. , Alrefaei Abdulwahed F. , Almutairi Bader O. , Rouabhia Mahmoud 
Published: May 2021

Abstract
The current study aimed to examine thymic stromal lymphopoietin receptor (TSLPR) genetic variation and breast cancer (BC) susceptibility in women in Saudi Arabia. Therefore, 127 blood samples from female patients diagnosed with BC and 116 blood samples from healthy female controls were studied using a genotyping assay to determine the association between three TSLPR single nucleotide polymorphisms (SNPs)—P196L, X201W, and A238V—and the risk of BC progression. In addition, gene expression was evaluated in 20 matching BC and normal tissues using immunohistochemistry. TSLPR protein levels were higher among BC patients than those with matching normal breast tissue. In addition, TSLPR SNP P196L was found to have a significant protective effect on BC progression (OR = 0.4427), although only the T allele for TSLPR P196L had this protective effect against BC progression in participants who were younger than 48 years old. In contrast, no association was found between the T allele and risk of BC in participants who were older than 48 years old, and the CT and TT genotypes were significantly associated with BC risk protection in the older group. The effects of the TT genotype and the T allele were closely associated with a decreased risk of BC in participants with estrogen receptors (ER+) and without them (ER-). Overall, the findings revealed a significant correlation between SNPs in the TSLPR genes and BC progression among women in Saudi Arabia.
A Signature of Seven Lipid Metabolism Genes to Predict Hepatocellular Carcinoma Prognosis
Authors: Li Muqi , Wu Xiwen , Liao Shufen , wang shutong , lin shuirong , song zimin , peng baogang , Shen Shunli 
Published: May 2021

Abstract
BackgroundLipid metabolism is important in tumor progression. However, its role in hepatocellular carcinoma (HCC) remains unknown. We attempt to build a lipid metabolism-related signature to evaluate its role in predicting the prognosis of HCC patients. MethodsWe obtained differential expression genes (DEGs) through differential analysis of mRNA expression between tumor tissues and paraneoplastic tissue of patients with HCC. The lipid metabolism-related genes were obtained from KEGG and MisDB. The corresponding gene expression and clinical data were acquired from The Cancer Genome Atlas (TCGA) database and the International Cancer Genome Consortium (ICGC) database. Prognosis-related genes were obtained by COX regression analysis. Intersecting genes were defined as genes shared by DEGs and prognosis-related genes. The least absolute shrinkage and selection operator (LASSO) technique was used to calculate the prognostic genes and coefficients for forming a prognostic assessment signature. Kaplan–Meier survival analysis was applied to assess the model’s credibility. ICGC database was also used for external validation. ResultsA total of 39 lipid metabolism-related DEGs were analyzed that showed significant enrichment in the phospholipid metabolic process, glycerolipid metabolic process and glycerophospholipid pathways. Seven lipid metabolism genes (ELOVL3, LCLAT1, ME1, PPARGC1A, PTDSS2, SRD5A3, SLC2A1) closely related with prognosis were identified to construct the signature. Patients with low-risk scores showed better survival rates, which was also validated in the ICGC database. ConclusionWe established a signature composed of seven lipid metabolism-related genes to predict the prognosis of HCC patients, providing a new biomarker for the diagnosis and treatment of HCC.
Links between gut dysbiosis and neurotransmitter disturbance in chronic restraint stress-induced depressive behaviours: the role of inflammation
Authors: Yang Hai-long , Li Meng-Meng , Zhou Man-Fei , Xu Huai-Sha , Huan Fei , Liu Na , Gao Rong , Wang Jun , Zhang Ning , Jiang Lei 
Published: May 2021

Abstract
Accumulating evidence has shown that inflammation, the gut microbiota and neurotransmitters are closely associated with the pathophysiology of depression. However, the links between the gut microbiota and neurotransmitter metabolism remain poorly understood. The present study aimed to investigate the neuroinflammatory reactions in chronic restraint stress (CRS)-induced depression and to delineate the potential links between the gut microbiota and neurotransmitter metabolism. C57BL/6 mice were subjected to chronic restraint stress for 5 weeks, followed by behavioural tests (the sucrose preference test, forced swim test, open field test and elevated plus maze) and analysis. The results showed that CRS significantly increased IL-1β, IL-2, IL-6 and TNFα levels and decreased BDNF expression, accompanied by the activation of IκBα-p-NF-κB signalling in the mouse hippocampus. In addition, the neurotransmitter metabolomics results showed that CRS resulted in decreased levels of plasma 5-HT, DA, and NE and their corresponding metabolites, and gut microbiota fecal metabolites with the 16S rRNA gene sequencing indicated that CRS caused marked microbiota dysbiosis in mice, with a significant increase in Helicobacter, Lactobacillus, and Oscillibacter and a decrease in Parabacteroides, Ruminococcus, and Prevotella. Notably, CRS-induced depressive behaviours and the disturbance of neurotransmitter metabolism and microbiota dysbiosis can be substantially restored by dexamethasone (DXMS) administration. Furthermore, a Pearson heatmap focusing on correlations between the microbiota, behaviours and neurotransmitters showed that Helicobacter, Lactobacillus, and Oscillibacter were positively correlated with depressive behaviours but were negatively correlated with neurotransmitter metabolism, and Parabacteroides and Ruminococcus were negatively correlated with depressive behaviours but were positively correlated with neurotransmitter metabolism. Taken together, the results suggest that inflammation is involved in microbiota dysbiosis and the disturbance of neurotransmitter metabolism in CRS-induced depressive changes, and the delineation of the potential links between the microbiota and neurotransmitter metabolism will provide novel strategies for depression treatment.
The effects of betamethasone on clinical outcome of the late preterm neonates born between 34 and 36 weeks of gestation
Authors: Arimi Yas , Zamani Narges , Shariat Mamak , Dalili Hossein 
Published: May 2021

Abstract
Background:
Prenatal corticosteroid administration in preterm labor is one of the most important treatments available to improve neonatal outcomes; however its beneficial effects on late preterm infants (after the 34th week of gestation) remained unknown. We aimed to assess the effects of betamethasone on the clinical condition of the late preterm infants born between 34 and 36 weeks of gestation.
Methods:
This retrospective cohort study was performed on 100 consecutive infants born between 34 and 36 weeks of gestation and received betamethasone before delivery as the cases and 100 neonates with the same delivery conditions but without receiving betamethasone. All neonates were followed up within hospitalization to assess the neonatal outcome.
Results:
The neonates receiving betamethasone suffered more from respiratory distress syndrome (49% versus 31%, p = 0.008) and requiring more respiratory support (71% versus 50%, p = 0.002) as compared to the control group. There was no difference between the two groups in other neonatal adverse events or death.
Conclusion:
the use of betamethasone in the late preterm period (after 34 weeks of gestation) has no beneficial effects on lung maturity or preventing neonatal adverse outcomes, even may lead to increase the risk for RDS and requiring respiratory support.
Theory of Real Relativity And E=Mc2 is Not Correct.
Authors: GAJENDRA GAJENDRA SHANKHWAR 
Published: May 2021

Abstract
This is a new theory of relativity, which we call the theory of real relativity. The total energy of a system is made up of the inherent energy and the external energy. Each of these energies is associated with its own coefficient. These coefficients are distributed throughout the universe in space and time, providing symmetry and stability to every visible, invisible, living, and non-living, conscious and unconscious of the universe. Hence the inherent energy coefficient (G1) and external energy coefficient (G2) are responsible for the change of universe and nature.
Incentive Preferences and Its Related Factors Among Primary Medical Staff in Anhui Province, China: A Cross-Sectional Study
Authors: Sang Lingzhi , Liu Hongzhang , Yan Huosheng , Rong Jian , Cheng Jing , Wang Li , Ding Hong , Chen Guimei , Chen Ren 
Published: May 2021

Abstract
Background The shortage of primary medical staff is a major problem in the management of health human resources across all countries. This study focused on the primary medical staff in Anhui Province. By determining their preferences for various motivational and related factors, we examined the correlation between staff’s motivation preference levels and staff turnover and turnover intention. This study aimed to further improve the incentive mechanism and to provide a reference for healthcare managers to formulate management strategies for the primary medical staff team.Methods A self-reported questionnaire survey was conducted to collect data. The basic survey content included demographic characteristics. The absolute level questionnaire and relative level questionnaire on the factors affecting motivation preference were used as the main assessment scales. A total of 1,112 primary-level medical workers in Anhui Province were investigated. T-test, analysis of variance (ANOVA), exploratory factor analysis, and multiple linear regression analysis were performed to analyze the data.Results The survey respondents (45.1%) reported being satisfied with their relationship with colleagues, and other social relationships (46.9 %). The Kaiser Meyer Olkin (KMO) value for the absolute preference degree for motivational factors was 0.951. Two factors (economic and non- economic factors), after using the maximum variance rotation axis method, explained 81.25% of the total variance. The regression analysis showed that primary medical staff members with high monthly income have a lower preference for non-economic factors; the higher the educational background, the higher their preference for economic factors. In addition, with the increase in participants’ age, the preference for motivational factors gradually decreased.Conclusion Both economic and non-economic factors play an important role in enhancing the enthusiasm of primary medical staff and improving their work attitude. Managers should use their influence to stabilize the primary medical staff.
The impact of intervention to the incidence of natural focus diseases during the outbreak of COVID-19 in Jiangsu Province, China
Authors: Cheng Xiaoqing , Hu Jianli , Luo Li , Zhao Zeyu , Zhang Nan , Hannah Mikah Ngwanguong , Rui Jia , Lin Shengnan , Zhu Yuanzhao , Wang Yao , Yang Meng , Xu Jingwen , Liu Xingchun , Yang Tianlong , Liu Weikang , Li Peihua , Deng Bin , Li Zhuoyang , Liu Chan , Huang Jiefeng , Bao Changjun , Chen Tianmu 
Published: May 2021

Abstract
Doxorubicin (DOX) is a well-known chemotherapeutic drug for most malgnencies including breast cancer and leukemia whilst the usage of DOX is limited owing to its cardiotoxicity. The present study analyzed the effects of crocin on doxorubicin’s cardiotoxic efect in rat myocardium and searched their mechanistic interaction in the pathogenesis of DOX-induced myocardial toxicity. Forty rats were divided into four groups; (a) control (received normal saline as a dose of 1 ml/kg by ip for 15 days), (b) Crocin (received crocin as a dose of 40 mg/kg/24h by ip for 15 days), (c) DOX (received DOX as a dose of 2 mg/kg/48h by ip in six injection, cumulative dose 12 mg/kg), and (d) DOX+Crocin (received DOX as a dose of 2 mg/kg/48h by ip in six injection and crocin as a dose of 40 mg/kg/24h ip for 15 days). According to the present study, DOX administration caused significant increases in lipid indices (triglyseride, low-dencity lipoproteins and very low-dencity lipoproteins) as well as cardiac markers (Creatine kinase-muscle/brain and Cardiac Troponin I). Morever, DOX caused significant increases in oxidative stress parameters (malondialdehyde and total oxidant status) as well as decreases in antioxidant defense systems (glutathione, superoxide dismutase, catalase and total antioxidant status). The present study also demonstrated that co-administration of crocin with DOX significantly ameliorated the lipid profile and biochemical parameters in rats receiving DOX. The results were supported by histopathological and immunohistochemical evaluations. Taken together, our results reveal that crocin might be a cardioprotective agent in DOX treated patients for cancer.
Validity, reliability, and ceiling and floor effects of the PROMIS Preference score (PROPr) in patients with rheumatological and psychosomatic conditions
Authors: Klapproth Christoph Paul , Fischer Felix , Merbach Marie , Matthias Rose , Obbarius Alexander 
Published: May 2021

Abstract
Background: The PROMIS Preference score (PROPr) is a new generic preference-based health-related quality of life (HRQoL) score that can be used as a health state utility (HSU) score for quality-adjusted life years (QALYs) in cost-utility analyses (CUAs). It is the first HSU score based on item response theory (IRT) and has favorable psychometric properties. The PROPr combines the seven PROMIS domains: cognition, depression, fatigue, pain, physical function, sleep disturbance, and ability to participate in social roles and activities. It was developed based on preferences of the US general population. We aimed to validate the PROPr in a patient sample and to compare it to the EQ-5D. Methods: We collected PROPr and EQ-5D-5L data from 141 patients treated in the rheumatology and psychosomatic departments. We evaluated the convergent validity, reliability, known-groups construct validity, and ceiling and floor effects of the PROPr and compared those characteristics to those of the EQ-5D. Results: The mean PROPr (0.26, 95% CI: 0.23; 0.29) and the mean EQ-5D (0.44, 95% CI: 0.38; 0.51) differed significantly (d = 0.18, p < 0.001). The Pearson correlation coefficient between the two scores was r = 0.72. The PROPr and EQ-5D demonstrated high reliability and similar discrimination power across sex, age, and conditions. While the PROPr showed a floor effect, the EQ-5D showed a ceiling effect. Conclusion: PROPr and EQ-5D measure HSU differently. The PROPr shows a broader definition of perfect health, with a wider range of measurements at the top end. The previous results of excellent validity and precision were confirmed.