bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomes derived from adipose-derived stem cells overexpressing glyoxalase-1 protect endothelial cells and enhance angiogenesis in type 2 diabetic mice with limb ischemia
Authors: Zhang Xing , Jiang Yihong , Huang Qun , Wu Zhaoyu , Pu Hongji , Xu Zhijue , Li Bo , Lu Xinwu , Yang Xinrui , Qin Jinbao , Peng Zhiyou 
Published: July 2021

Abstract
Background
Diabetic limb ischemia is a clinical syndrome and refractory to therapy. Our previous study demonstrated that adipose-derived stem cells (ADSCs) overexpressing glyoxalase-1 (GLO-1) promoted the regeneration of ischemic lower limbs in diabetic mice, but low survival rate, difficulty in differentiation, and tumorigenicity of the transplanted cells restricted its application. Recent studies have found that exosomes secreted by the ADSCs have the advantages of containing parental beneficial factors and exhibiting non-immunogenic, non-tumorigenic, and strong stable characteristics.

Methods
ADSCs overexpressing GLO-1 (G-ADSCs) were established using lentivirus transfection, and exosomes secreted from ADSCs (G-ADSC-Exos) were isolated and characterized to coculture with human umbilical vein endothelial cells (HUVECs). Proliferation, apoptosis, migration, and tube formation of the HUVECs were detected under high-glucose conditions. The G-ADSC-Exos were injected into ischemic hindlimb muscles of type 2 diabetes mellitus (T2DM) mice, and the laser Doppler perfusion index, Masson’s staining, immunofluorescence, and immunohistochemistry assays were adopted to assess the treatment efficiency. Moreover, the underlying regulatory mechanisms of the G-ADSC-Exos on the proliferation, migration, angiogenesis, and apoptosis of the HUVECs were explored.

Results
The G-ADSC-Exos enhanced the proliferation, migration, tube formation, and anti-apoptosis of the HUVECs in vitro under high-glucose conditions. After in vivo transplantation, the G-ADSC-Exo group showed significantly higher laser Doppler perfusion index, better muscle structural integrity, and higher microvessel’s density than the ADSC-Exo and control groups by Masson’s staining and immunofluorescence assays. The underlying mechanisms by which the G-ADSC-Exos protected endothelial cells both in vitro and in vivo might be via the activation of eNOS/AKT/ERK/P-38 signaling pathways, inhibition of AP-1/ROS/NLRP3/ASC/Caspase-1/IL-1β, as well as the increased secretion of VEGF, IGF-1, and FGF.

Conclusion
Exosomes derived from adipose-derived stem cells overexpressing GLO-1 protected the endothelial cells and promoted the angiogenesis in type 2 diabetic mice with limb ischemia, which will be a promising clinical treatment in diabetic lower limb ischemia.
Divergence in chondrogenic potential between in vitro and in vivo of adipose- and synovial-stem cells from mouse and human
Authors: Chijimatsu Ryota , Miwa Satoshi , Okamura Gensuke , Miyahara Junya , Tachibana Naohiro , Ishikura Hisatoshi , Higuchi Junya , Maenohara Yuji , Tsuji Shinsaku , Sameshima Shin , Takagi Kentaro , Nakazato Keiu , Kawaguchi Kohei , Yamagami Ryota , Inui Hiroshi , Taketomi Shuji , Tanaka Sakae , Saito Taku 
Published: July 2021

Abstract
Background
Somatic stem cell transplantation has been performed for cartilage injury, but the reparative mechanisms are still conflicting. The chondrogenic potential of stem cells are thought as promising features for cartilage therapy; however, the correlation between their potential for chondrogenesis in vitro and in vivo remains undefined. The purpose of this study was to investigate the intrinsic chondrogenic condition depends on cell types and explore an indicator to select useful stem cells for cartilage regeneration.

Methods
The chondrogenic potential of two different stem cell types derived from adipose tissue (ASCs) and synovium (SSCs) of mice and humans was assessed using bone morphogenic protein-2 (BMP2) and transforming growth factor-β1 (TGFβ1). Their in vivo chondrogenic potential was validated through transplantation into a mouse osteochondral defect model.

Results
All cell types showed apparent chondrogenesis under the combination of BMP2 and TGFβ1 in vitro, as assessed by the formation of proteoglycan- and type 2 collagen (COL2)-rich tissues. However, our results vastly differed with those observed following single stimulation among species and cell types; apparent chondrogenesis of mouse SSCs was observed with supplementation of BMP2 or TGFβ1, whereas chondrogenesis of mouse ASCs and human SSCs was observed with supplementation of BMP2 not TGFβ1. Human ASCs showed no obvious chondrogenesis following single stimulation. Mouse SSCs showed the formation of hyaline-like cartilage which had less fibrous components (COL1/3) with supplementation of TGFβ1. However, human cells developed COL1/3+ tissues with all treatments. Transcriptomic analysis for TGFβ receptors and ligands of cells prior to chondrogenic induction did not indicate their distinct reactivity to the TGFβ1 or BMP2. In the transplanted site in vivo, mouse SSCs formed hyaline-like cartilage (proteoglycan+/COL2+/COL1−/COL3−) but other cell types mainly formed COL1/3-positive fibrous tissues in line with in vitro reactivity to TGFβ1.

Conclusion
Optimal chondrogenic factors driving chondrogenesis from somatic stem cells are intrinsically distinct among cell types and species. Among them, the response to TGFβ1 may possibly represent the fate of stem cells when locally transplanted into cartilage defects.
Rabeprazole inhibits inflammatory reaction by inhibition of cell pyroptosis in gastric epithelial cells
Authors: Xie Jing , Fan Long , Xiong Liya , Chen Peiyu , Wang Hongli , Chen Huan , Zhao Junhong , Xu Zhaohui , Geng Lanlan , Xu Wanfu , Gong Sitang 
Published: July 2021

Abstract
Background
Helicobacter pylori (H. pylori) is a common pathogen in development of peptic ulcers with pyroptosis. Rabeprazole, a critical component of standard triple therapy, has been widely used as the first-line regimen for H. pylori infectious treatment. The aim of this study to explore the function of Rabeprazole on cell pyroptosis in vitro.

Methods
The clinical sample from patients diagnosed with or without H. pylori-infection were collected to analyze by Immunohistochemistry (IHC). Real-time quantitative PCR (qPCR), western blot (WB) and enzyme linked immunosorbent assay (Elisa) were performed to analyze the effect of Rabeprazole on cell pyroptosis, including LDH, IL-1β and IL-18.

Results
In this study, we showed that Rabeprazole regulated a phenomenon of cell pyroptosis as confirmed by lactate dehydrogenase (LDH) assay. Further results showed that Rabeprazole inhibited cell pyroptosis in gastric epithelial cells by alleviating GSDMD-executed pyroptosis, leading to decrease IL-1β and IL-18 mature and secretion, which is attributed to NLRP3 inflammasome activation inhibition. Further analysis showed that ASC, NLRP3 and Caspase-1, was significantly repressed in response to Rabeprazole stimulation, resulting in decreasing cleaved-caspase-1 expression. Most important, NLRP3 and GSDMD is significantly increased in gastric tissue of patients with H. pylori infection.

Conclusion
These findings revealed a critical role of Rabeprazole in cell pyroptosis in patients with H. pylori infection, suggesting that targeting cell pyroptosis is an alternative strategy in improving H. pylori treatment.
Risk factors for overweight and obesity among women of reproductive age in Dar es Salaam, Tanzania
Authors: Mosha Dominic , Paulo Heavenlight A. , Mwanyika-Sando Mary , Mboya Innocent B. , Madzorera Isabel , Leyna Germana H. , Msuya Sia E. , Bärnighausen Till W. , Killewo Japhet , Fawzi Wafaie W. 
Published: July 2021

Abstract
Background
Overweight and obesity have increased considerably in low- and middle-income countries over the past few decades, particularly among women of reproductive age. This study assessed the role of physical activity, nutrient intake and risk factors for overweight and obesity among women in Dar es Salaam, Tanzania.

Methods
We conducted a cross-sectional survey among 1004 women aged 15–49 years in the Dar es Salaam Urban Cohort Study (DUCS) from September 2018 to January 2019. Dietary intake was assessed using a food frequency questionnaire (FFQ). Physical activity was assessed using the Global Physical Activity Questionnaire (GPAQ) using metabolic equivalent tasks (MET). Modified poison regression models were used to evaluate associations between physical activity and nutrient intake with overweight/obesity in women, controlling for energy and other factors.

Results
The mean (±SD) age of study women was 30.2 (±8.1) years. Prevalence of overweight and obesity was high (50.4%), and underweight was 8.6%. The risk of overweight/obesity was higher among older women (35–49 vs 15–24 years: PR 1.59; 95% CI: 1.30–1.95); women of higher wealth status (PR 1.24; 95% CI: 1.07–1.43); and informally employed and married women. Attaining moderate to high physical activity (≥600 MET) was inversely associated with overweight/obesity (PR 0.79; 95% CI: 0.63–0.99). Dietary sugar intake (PR 1.27; 95% CI: 1.03–1.58) was associated with increased risk, and fish and poultry consumption (PR 0.78; 95% CI: 0.61–0.99) with lower risk of overweight/obesity.

Conclusion
Lifestyle (low physical activity and high sugar intake), age, wealth status, informal employment and marital status were associated with increased risk of overweight/obesity, while consumption of fish and poultry protein was associated with lower risk. The study findings underscore the need to design feasible and high-impact interventions to address physical activity and healthy diets among women in Tanzania.
Two-dimensional simulation of frost formation on the NACA0012 airfoil under strong convection by using the p-VOF method
Authors: Xia Bin , Xu Xianghua , Liang Xingang 
Published: July 2021

AbstractA newly developed frosting simulation method, p-VOF method, is applied to simulate the dynamic frost formation on the NACA0012 airfoil under strong convection. The p-VOF method is a pseudo VOF method of the multiphase flow simulation with phase change. By solving a simplified mass conservation equation explicitly instead of the original volume fraction equations in CFD software, the efficiency and robustness of calculation are greatly improved. This progress makes it possible to predict a long-time frost formation. The p-VOF method was successfully applied to the simulation of dynamic frosting on the two-dimensional NACA0012 airfoil under strong convection conditions with constant frost physical properties. The simulation result shows that the average thickness of the frost layer increases, and the frost bulges and flow separation appear earlier, when the airfoil surface temperature decreases or the air humidity increases. The frost bulges and flow separation appear earlier, when the air velocity is faster, the growth rate of the frost layer at the early stage is greater, but the final frost layer is thinner.
A chrome-free combination tanning strategy: based on silicic acid and plant tannin
Authors: Zhang Zetian , Liu Yang , Wang Junchao , Xie Taoling , Sun Liying , Li Zhengjun 
Published: July 2021

AbstractSilicic acid, commonly derived from cheap and easily available sodium silicate, has recently received great attention for application in leather industry to produce ecological leather with a cleaner approach. However, leather tanned with silicic acid alone is poor in storage stability, which limits its practical application in leather production. In this work, a new environment-friendly combination tannage based on silicic acid and plant tannin was developed to address this issue along with improving the comprehensive performances of leather. The obtained leather was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and so on. The results showed that compared with leather tanned with silicic acid alone, the leather tanned with this combination method possessed improved thermal stability, enhanced mechanical properties, acceptable softness, appropriate hydrophilicity, and especially enhanced storage stability. More importantly, the combination tanned leather with 1:1 of the mass ratio of silicic acid to vegetable tannin (composed of valonea extract and mimosa extract with the same weight) had more prominent comprehensive performances. In addition, the results demonstrated that hydrogen bonding played an important role in the combination tanning process. Furthermore, the hydrogen bonds generated between phenolic hydroxyl groups of polyphenols with silicon hydroxyl groups of silicic acid molecules inhibited the excessive condensation of Si-OH groups between themselves. Subsequently, the assessment of environmental impact revealed the value of BOD5/COD of the wastewater produced in this combination tanning process is more than 0.3, indicating the chrome-free combination tannage based on silicic acid and plant tannin was an environment-friendly tanning technology. These findings therefore indicated that a new chrome-free tanning method with silicon and biomass materials as main tanning agents has potential practical application prospect in leather production.Graphical abstract
Burden of Risk Factors for Cervical Cancer Among Women Living in East Africa: An Analysis of the Latest Demographic Health Surveys Conducted Between 2014 and 2017
Authors: Abila Derrick Bary , Wasukira Sulaiman Bugosera , Ainembabazi Provia , Wabinga Henry 
Published: July 2021

PURPOSE In East Africa, cervical cancer is a leading cause of morbidity and mortality among women diagnosed with cancer. In this study, we describe the burden of risk factors for cervical cancer among women of reproductive age in five East African countries. METHODS For each country, using STATA13 software and sampling weights, we analyzed the latest Demographic and Health Survey data sets conducted between 2014 and 2017 in Burundi, Kenya, Rwanda, Tanzania, and Uganda. We included women age 15-49 years and considered six risk factors (tobacco use, body mass index, age at first sexual intercourse, age at first birth, number of children, and hormonal contraceptive use). RESULTS Of the 93,616 women from the five countries, each country had more than half of the women younger than 30 years and lived in rural areas. Pooled proportion of women with at least one risk factor was 89% (95% CI, 87 to 91). Living in a rural area in Burundi (adjusted incidence rate ration 0.94; 95% CI, 0.9 to 0.99; P = .019) and Rwanda (adjusted incidence rate Ration 0.92; 95% CI, 0.88 to 0.96; P < .001) was associated with a lower number of risk factors compared with living in an urban area. In all the countries, women with complete secondary education were associated with a lower number of risk factors compared with those with no education. CONCLUSION This study reveals a high burden of risk factors for cervical cancer in East Africa, with a high proportion of women exposed to at least one risk factor. There is a need for interventions to reduce the exposure of women to these risk factors.
Serpouhi Dussap’s Mayda; or, The Birth of Armenian Women’s Literature through the Palimpsestic Narrative of Feminism
Authors: Aktokmakyan Maral 
Published: July 2021

Abstract
Mayda (1883), Serpouhi Dussap’s first eponymous novel, quickly met the patriarchal reaction among the Armenian male intelligentsia of Constantinople over the issue of female emancipation. Today the significance of Dussap’s best-known novel and feminist ideology is both welcome and appreciated yet not with much gratifying analysis of the literary and intellectual ventures invested in this first-time production. This article seeks to place this literary event among the feminist literature scholarship of the nineteenth century. For this initial venture to bring recognition to Armenian feminist literature, the present study introduces the general literary scene in the Ottoman Armenian community in the late nineteenth century, then outlines the critical detraction leveled against the novel, only to produce a new reading that reflects the feminist literary strategy of the time, namely, the palimpsestic mode of writing. Finally, the reading suggested in this article is linked to another underrated work, the mythical tale of Arachne. Reworking Nancy K. Miller’s theory of “Arachnologies” into the context of Armenian women’s literature, the analysis of Arachne’s tale is the “mytheme,” the foundational unit, so to speak, that encapsulates the feminist literary ideology of nineteenth-century Armenian women’s literature.
Postfeminism à la Turca
Authors: Özdemir Burcu Dabak 
Published: July 2021

Abstract
This essay analyzes how postfeminism is constructed on a visual level in the Turkish context. It uses theories of postfeminism to discuss new popular romantic comedies of Turkish cinema by comparing the new female protagonists with the women portrayed in Yeşilçam melodramas. Three films—Kocan Kadar Konuş (dir. Kıvanç Baruönü, 2014), Hadi İnşallah (dir. Ali Taner Baltacı, 2014), and Aşk Nerede? (dir. Semra Dündar, 2015)—are analyzed from a postfeminist perspective, opening up a new scholarly discussion about the place of postfeminism in the Turkish cinema. These films represent what may be termed “Turkified” postfeminism, which has been commonly discussed for the Western world but not sufficiently taken into account for Middle Eastern women.
Relative advantages and compatibility of a biometric patient identification tool in Zambia: a qualitative analysis
Authors: Lissin Polina , Hamapa Arnold , Kobayashi Misaki , Smith-Sreen Joshua , Etter Lauren , Pieciak Rachel , Mukuka Euphrasia , Mumba Zulu Joseph , Michelo Charles , Mwananyanda Lawrence , Gill Chris J. 
Published: July 2021

Background: The Scanning Ears for Child Health (SEARCH) system is a biometric patient identification tool which uses a pattern recognition algorithm to translate an image of the ear into a unique identifier. If integrated into an electronic medical records (EMR) system, it would provide a patient identification solution that replaces unreliable paper, under-five card (UFC), or number-based identifiers. This study aims to understand the relative advantage of the biometric system, the sociocultural and pragmatic compatibility, and the extent of UFC deterioration over time. Methods: Interviews on impressions of the novel biometric patient identification tool were conducted in urban and rural settings in Zambia. Focus group discussions included 59 participants and key informant interviews included 5 healthcare workers and 2 government officials. Transcripts were coded into thematic categories for analysis. We sought to understand 1) the perceived relative advantage of a biometric system over the traditional UFCs among Zambian mothers, 2) the perceived sociocultural compatibility of a biometric system in the healthcare setting, and 3) pragmatic compatibility of the proposed system. Results: We found that the current UFC system presents issues for continuity of care and quality of data management, therefore posing disadvantages relative to the proposed system. Sociocultural and pragmatic barriers to acceptance included the existing fear of Satanism and electrical power issues throughout Zambia. Mothers and healthcare workers expressed that adoption of the biometric system could be successful given efforts to sensitize the community. Conclusions: Switching to an EMR backed by biometric identification would fill a critical gap in Zambian healthcare information systems and has numerous perceived advantages in both urban and rural settings. We determine that strategies for implementation should be localized, context informed, and conducted by trusted community members with knowledge of best approaches to diffusing information and a deep understanding of the novel biometric system.
Large to massive rotator cuff tendon tears: a protocol for a systematic review investigating the effectiveness of exercise therapy on pain, disability and quality of life
Authors: Fahy Kathryn , Galvin Rose , Lewis Jeremy , McCreesh Karen 
Published: July 2021

Background: Rotator cuff tendon tears are inextricably linked with the natural process of aging often resulting in severe disability, poor quality of life and an added burden to the health care system. The occurrence of rotator cuff tendon tears increases exponentially with every decade of life to approximately 60% in individuals over 80 years of age. Exercise is a commonly prescribed intervention although research on its efficacy is in its infancy and often conflicting. The purpose of this systematic review is to investigate the effectiveness of exercise interventions for people diagnosed with large to massive rotator cuff tendon tears. Methods: This systematic review will adhere to the PRISMA reporting guidelines. A comprehensive search of five databases will be conducted. Randomised clinical trials (RCT) or quasi-randomised control trials will be included if they evaluate exercise as the core intervention or as part of the intervention in the management of large to massive rotator cuff tears. To quantify response to treatment we will compare changes in pain, disability and quality of life (QoL). The Consensus on Exercise Reporting Template (CERT) will be used to characterise the different types of exercise intervention. The Cochrane Risk of Bias Tool will be used to assess study quality. A narrative synthesis with meta-analysis will be performed, and the certainty of evidence will be assessed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) criteria. Discussion: This review will synthesise the totality of GRADE A and B evidence on the effectiveness of exercise for large to massive rotator cuff tendon tears. It will provide clinically important information and guidance for immediate implementation by clinicians, health policymakers and may be used to guide future research. PROSPERO registration: 244502 (24/03/2021)
Ensinar histórias menores: narrativas e memórias de auxiliares de serviços gerais escolares na busca de relações outras
Authors: Paladini Piazza Maria Cecília , Santana Giovanna 
Published: July 2021

Este artigo dialoga com narrativas de quatro mulheres auxiliares de serviços gerais de uma escola estadual no município de Araranguá, Santa Catarina. O objetivo de escutá-las surge como uma maneira de disseminar outra postura epistemológica em relação aos conhecimentos tradicionalmente valorizados pelos currículos oficiais, buscando com apoio do ensino de História evidenciar suas contribuições como educadoras. O estudo se desenvolveu a partir da análise de fontes narrativas, bibliográficas e documentais. Metodologicamente está fundamentado na concepção de mônada, extraída do pensamento de Walter Benjamin, e impulsionado por leituras decoloniais. Considera-se que é possível estimular dentro da perspectiva historiográfica uma Pedagogia da Memória, com a insurgência de histórias menores em contraposição ao pensamento hegemônico da história maior, narrativa estrutural distante da realidade dos estudantes e dos sujeitos presentes na comunidade escolar.