bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship Between Satisfaction With Mental Health Services, Personal Recovery and Quality of Life Among Service Users With Psychosis: A Cross-Sectional Study
Authors: Skar-Fröding Regina , Clausen Hanne Kristin , Benth Jūratė Šaltytė , Ruud Torleif , Slade Mike , Heiervang Kristin Sverdvik 
Published: January 2021

Abstract
Background: Mental health policy internationally emphasizes patient centredness and personal recovery. This study investigated the relationship between satisfaction with mental health services among service users with psychosis in Norway, and personal recovery, perceived support for personal recovery, and quality of life.Methods: Cross-sectional data were collected from 292 service users diagnosed with psychosis from 39 clinical sites across Norway. Satisfaction with services was assessed using the Client Satisfaction Questionnaire-8. A linear mixed model was estimated to explore the relationship between satisfaction with services and preselected covariates, and to control for confounding factors. Results: A large majority of participants (89%) reported moderate-to-high levels of satisfaction. Satisfaction with services was positively associated with perceived support for personal recovery, but not with personal recovery or quality of life. In addition, service users under a Community Treatment Order (CTO) were significantly less satisfied than those who were not. Conclusions: Satisfaction levels among service users were higher compared with similar, international studies. Those who feel supported in their personal recovery were more satisfied with the care they receive, which support the need for implementation of recovery-oriented practices for service users with psychosis. However, satisfaction with services was not related to service user-rated quality of life or level of personal recovery; thus, more follow-up studies are needed. The lower satisfaction of service users placed under CTOs shows the importance of targeted interventions to improve satisfaction with services among this group. Trial registration: NCT03271242, date of registration: 5 sept. 2017
New 5-LOX and 15-LOX Inhibitors Isolated from Red Sea Derived Brown Alga Sarragassum Cinnerum: An In Silico-Supported Study
Authors: Alzarea Sami I. , Elmaidomy Abeer H. , Saber Hani , Musa Arafa , Al-Sanea Mohammad M. , Mostafa Ehab M. , Hendawy Omnia M. , Youssif Khayrya A. , Alanazi Abdullah S. , Alharbi Metab , Sayed Ahmed M. , Abdelmohsen Usama Ramadan 
Published: January 2021

Abstract
Sargassum is a brown algal genus inhabiting tropical region. Metabolomic profiling of Sarragassum cinnerum “Sargassaceae”, dereplicated eleven compounds 1-11, further phytochemical investigation afforded two new aryl cresol 12-13, along with eight known compounds 14-21. Both new metabolites along with 19 showed moderate in vitro antiproliferative activity against HEPG2, MCF7, and CACO2. Molecular targets of the bioactive compounds using a pharmacophore-based virtual screening, predicts 5-LOX and 15-LOX as the most probable target linked to their observed antiproliferative activity. The validation step revealed 12 and 13 inhibited 5-LOX more prudentially than 15-LOX, while 19 showed a convergent inhibitory activity toward both enzymes. Further in-depth in silico analysis revealed the molecular interactions inside both enzymes active sites and explained the varying inhibitory activity for 12, and 13 toward 5-LOX and 15-LOX. Taken together, unique metabolites in S. cinnerum had potential anticancer activity supported with in-silico investigations to facility drug discovery and development processes.
Identification of weak non-canonical base pairs around riboswitch-ligand recognition sites by solid-state NMR exchange spectroscopy
Authors: Zhao Sha , Wen Ziyang , Li Xinming , zou Mengbing , Yu Ge , Liu Xiangyang , Zhang Lixin , Xue Yi , Fu Riqiang , Wang Shenlin 
Published: January 2021

Abstract
Base pairs are fundamental building blocks of RNA structures, and their stability and open-close equilibrium constitutes the dynamic picture. Weak base pairs, which feature the characteristics of low stability and rapid base pair opening, often play a critical role in RNA functions. However, site-specific identification of weak base pairs in RNA is challenging. Here, we report a solid-state NMR (SSNMR)-based two-dimensional proton-detected water–RNA exchange spectroscopy (WaterREXSY) to address this challenge. The approach uses the chemical exchange between hydrogen-bonded imino protons within the base pair and excited water molecules to polarize the imino protons for SSNMR observation. This process takes advantages that the imino protons within weak pairs undergo fast exchange rates with water, enabling a quick build-up and efficient detection. This method is used to characterize the weak pair in the riboA71–adenine complex (i.e., the 71nt-aptamer domain of the add adenine riboswitch from Vibrio vulnificus). We identify U47•U51, a weak non-canonical base pair that constitutes the U47•U51•(adenine-U74) base tetrad around the ligand-binding pocket. This result suggests that the breakage of U47•U51 may be the early stage in the process of ligand release.
Evaluation of A Simulation-Based Clinical Preparation Program by Final-Year Medical Students: A Qualitative Study on Emergency Patient Management
Authors: Kitapcioglu Dilek , Tanriover Ozlem , Inanici Sinem Yildiz , Aksoy Mehmet Emin , Gulpinar Mehmet Ali 
Published: January 2021

Abstract
Background: The most common problem encountered by medical students is the management of unstable emergency patients. Simulation-based education (SBE) is widely used for improving the management of unstable patients. In this study, the aim is to evaluate the SBE Clinical Preparation Program and to understand whether our final-year medical students could transfer the skills acquired in the program to clinical encounters in their emergency medicine clerkship, with the aim of easing this transition.Materials and methods: Final-year medical students at the Acıbadem Mehmet Ali Aydınlar University Faculty of Medicine and their educators were included in this qualitative study. After participating in the SBE Clinical Preparation Program for two weeks, interns completed a two-month emergency medicine clerkship. At the end of the clerkship, four focus group discussions with 5 or 7 interns (n=24) and in-depth interviews with two educators were held. The digital audio recordings were transcribed verbatim within 48 hours of the interviews. The texts were reviewed by two different researchers, and thematic codes were identified.Results: In this study, the effectiveness of the SBE Clinical Preparation Program was evaluated. The participants stated that the program prepared them very well for critical patient management and that the content of the program was sufficient and comprehensive. The trainers stated that the simulation program prepared the students well for the clinic and provided a strong foundation, which was very important, but the students had to overcome an emotional barrier when approaching real patients, a process that took one or two weeks. The characteristics of the students (motivation, anxiety levels), the quality of the processes experienced during the program (seeing a sufficient number of patients, communication and interaction with other healthcare professionals and patients) and the environment/context in which these practices took place (learning climate) were the important factors influencing the transfer of the learning outcomes to the clinical environment.Conclusion SBE transition programs are effective in preparing students for the management of critical patients and facilitate the transition to the clerkship. SBE programs are not sufficient by themselves and should be developed in a continuous and integrated manner with training programs in actual clinical settings.
QTL Mapping for the Starch Paste Viscosity of Rice (Oryza Sativa L.) Using a Chromosome Segment Substitution Lines Derived From Two Sequenced Cultivars With Same Wx Allele
Authors: Zhao Ling , Zhao Chunfang , Zhou Lihui , Zhao Qingyong , Zhu Zhen , Chen Tao , Yao Shu , Zhang Yadong , Wang Cailin 
Published: January 2021

Abstract
BackgroundEating and cooking qualities (ECQs) of rice (Oryza sativa L.) is one of the most important economic characters which affecting production and market value. The starch viscosity profile tested by Rapid visco analyzer (RVA) is the direct measure of ECQs that represents the changes in viscosity associated with starch gelatinization. RVA profiles of rice are controlled by a complex genetic system and affected by environment. It is necessary to identify genetic factors controlled the RVA profile characters for development of rice varieties with excellent ECQs. Although it is known that Waxy (Wx) is the major gene control the amylose content (AC) and ECQs, there still have other unknown genetic factors effecting on ECQs.ResultsQuantitative trait loci (QTL) for starch paste viscosity of rice was analyzed using chromosome segment substitution lines (CSSLs) developed from 9311 and Nipponbare with same Wx-b background. With this CSSLs, the effect of major locus Wx could be eliminated and the other locus associated with RVA profile could be identified. QTLs for seven traits of starch RVA profile were tested over 4 years in Nanjing, China. 310 QTLs were identified (from 1 to 55 QTLs for each trait). Among 136 repeated QTLs, 6 QTLs were stable and detected every year. In total of 26 marker intervals detected over 3 years or more, 13 showed pleiotropy with respect to controlling 2 to 6 starch RVA profile properties simultaneously. The stable QTL clusters which we mapped here were overlapped to some of the known starch synthesis–related genes (SSRGs). ConclusionsWithout the effect of major locus Wx, many QTLs associated with RVA profile of rice were identified and some of them were stable. Our study illustrated that the hereditary of rice starch RVA profile is complicated, pleiotropic effects and QTL hot spots are the key factors affecting starch RVA traits.
Pivotal Role of Dosimetry in High Activity 131i Mibg Therapy in Patients Affected by High Risk Relapsed Refractory Neuroblastoma: Towards a Deeper Metastatic Response Understanding
Authors: Cassano Bartolomeo , Pizzoferro Milena , Valeri Silvio , Polito Claudia , Donatiello Salvatore , Altini Claudio , Villani Maria Felicia , Castellano Aurora , Serra Annalisa , Locatelli Franco , Garganese Maria Carmen , Cannatà Vittorio 
Published: January 2021

Abstract
Background: Dosimetry in molecular radiotherapy for personalized treatment is assuming a central role in clinical management of aggressive/relapsed tumors. High-Risk relapsed/refractory metastatic Neuroblastoma (HRrrm-NBL) has a poor prognosis and high-activity 131I-mIBG therapy could represent a promising strategy.The aim of this study was to report the absorbed dose to whole-body (DWB), red marrow (DRM) and lesions (DLesion) correlated to clinical outcome.Methods: 14 patients affected by HRrrm-NBL were treated with high-activity 131I-mIBG therapy (two administrations separated by 15 days). The first administration was weight-based whereas the second one was dosimetry-based (achieving 4 Gy to whole-body). In all patients DWB and DRM has been assessed; 9/14 patients were selected for DLesion evaluation (for a total of 13 lesions). Treatment response was classified in Progression and Stable Disease (PD and SD), Partial and Complete Response (PR and CR) and correlated to the value of DLesion value.Results: The cumulative DWB, DRM and DLesion ranged from [1.49; 4.45], [0.99; 2.56] and [44.17; 585.75] Gy. After treatment 3, 2, 4 and 5 patients showed CR, PR, SD and PD respectively showing a correlation between the clinical outcome and DLesion with a threshold at 80 Gy.Conclusions: Our experience shows feasibility of high activity therapy of 131I MIBG in rrmHR-NBL children as two administration intensive strategy. Dosimetric approach allowed a tailored high dose treatment maximizing the benefits of radionuclide therapy for pediatric patients with a safety profile. The assesment of DLesion contributed to have a deeper understaning of metabolic treatment effects.
The Combination of G-CSF/AMD3100 Mobilizes Bone Marrow-Derived Stem Cells to Rescue Mice from Cisplatin-Induced Acute Kidney Injury
Authors: Chen Zhi , Ren Xiang , Ren Ruimin , Wang Yonghong , Shang Jiwen 
Published: January 2021

Abstract
Background: Several studies have confirmed that mobilizing bone marrow-derived stem cells (BMSCs) ameliorates renal function loss following cisplatin-induced acute kidney injury (AKI). The aim of this study was to explore whether the combination of G-CSF/AMD3100 exerts beneficial effects with respect to renal function recovery in a mouse model of cisplatin-induced nephrotoxicity. Methods: C57BL/6J mice received intraperitoneal injections of G-CSF (200 μg/kg/d) for 5 consecutive days. On the day of the last injection, the mice received a single subcutaneous dose of AMD3100 (5 mg/kg) 1 hour before cisplatin 20 mg/kg injection. 96 hours after cisplatin injection, the mice were euthanized, blood and tissue samples were collected to assess renal function and tissue damage. Cell mobilization was assessed by flow cytometry. Results: Mice pretreated with G-CSF/AMD3100 exhibited longer survival and significantly lower serum creatinine and BUN levels than mice treated with only G-CSF or saline, exhibited attenuated tissue injury and cell death and enhanced tissue repair and cell regeneration. C57BL/6J mice pretreated with G-CSF/AMD3100 exhibited higher numbers of stem cells in peripheral blood than mice treated with only G-CSF or saline. Furthermore, G-CSF/AMD3100 administration prevented increases in the expression of proinflammatory factors, such as IL-6 and TNF-α, and increased the expression of the anti-inflammatory factor IL-10. Conclusions: These results suggest that G-CSF/AMD3100 mobilizes bone marrow cells to improve renal function and prevent cisplatin-induced renal tubular injury and that the combination of G-CSF/AMD3100 is superior to G-CSF alone for preventing AKI. This combination may represent a new therapeutic option for the treatment of AKI and warrants further investigation.
The Long-term Results of Total Knee Arthroplasty in Octogenarian
Authors: Mak Rex Wang-Fung , Chau Wai-Wang , Chung Kwong-Yin , Chiu Kwok-Hing , Ho Kevin Ki-Wai 
Published: January 2021

Abstract
Background: With increasing life expectancy and aging population, more octogenarians would benefit from total knee arthroplasty (TKA). The aim of this study is to evaluate the survivorship of TKA in octogenarian and their long-term outcomes. Methods: This was a retrospective review of 67 patients aged above 80 years who had primary TKA performed between 2005-2013 at a single centre. A sex-matched younger cohort of 67 primary TKAs during the same time-period was recruited for control. 5- and 10-year survival was calculated. Pre- and peri-operative factors were collected and compared. Post-operative range of motion, Knee Society Score (KSS) and Function Score (KFS) were collected and compared. Rate of revision, infection, aseptic loosening and mortality was collected.Results: The mean follow-up period was 10.39±2.29 years. The 5- and 10- year survival of octogenarian group was 85.1% and 54.6% respectively. Pre- and peri-operative factors between the octogenarian and control groups were comparable (p>0.05). Both groups showed improvement in range of motion, KSS and KFS post-operatively. Rate of revision, aseptic loosening and deep infection was 0% in octogenarian and 1.5% in control group. Conclusions: TKA can be performed in patients with age above 80 years old with comparable post-op knee range, complication rate and long-term functional score. Advanced age should not be a limiting factor for TKA.
Occupational Stress Faced by Doctors of Public and Private Sector of Pakistan: Comparative Study
Authors: Namood-e-Sahar  , Saman Maira , Malik Meerab 
Published: January 2021

Abstract
The survey on comparative account of occupational role stress among doctor of public and private sectors was conducted by using the Occupational Role Stress Scale (ORS), devised by Pareek (1983). Sample comprised of doctors from public (n= 30) and from private sector (n= 30). The data obtained was statistically analyzed by taking the mean of scores obtained by the doctors of both sectors, for public doctors the arithmetic mean was (M= 27.13) and for private doctors the scores were (M= 48.8). So, it was clear that mean score for occupational stress in private sector was more as compared to the Public one and thus research hypothesis was accepted.
Purification, characterization and application of thermoalkaliphilic proteases from Bacillus filamentosus, Lysinibacillus cresolivorans, and Bacillus subtilis
Authors: Ullah Inam , Ali Nawab , Ullah Waheed , Qasim Muhammed , Nughman Muhammed , Ullah Nimat , Rashid Muhammed 
Published: January 2021

Abstract
Thermophilic proteases are important industrial enzymes because they can be used at unavoidable temperature in various bioprocessing schemes. The bacterial population of Cholistan desert was explored for thermophillic proteases and their industrial applications. Three bacterial isolates K1, K5, and K7 were found promising protease producers. These isolates were preliminary identified as Bacillus based on morphological characteristics and biochemical tests (positive for catalase, oxidase, and citrate tests, and negative for indole and urease tests). The isolates K1, K5, and K7 were further identified as Bacillus filamentosus, Lysinibacillus cresolivorans, and Bacillus subtilis, respectively by phylogenetic analysis. The isolates grew best at 50oC while Bacillus filamentosus (K1), Lysinibacillus cresolivorans (K5), and Bacillus subtilis (K7) produced larger zones of hydrolysis at 37oC, 45oC and 50oC at pH 7, respectively. The optimum temperature for protease activity was 65oC for Bacillus filamentosus and Lysinibacillus cresolivorans and 55oC for Bacillus subtilis, and the optimum pH for activity was 9 for all the three strains. The protease produced by these isolates were found active at high temperature (37oC to 85oC) and high pH (5–12) which make them industrially important thermoalkaliphilic proteases. Theses proteases successfully de-haired cow’s skin and de-stained blood from cotton cloth pieces, which are rarely tested applications of these proteases.
Utilising Synergism Between the Transverse Abdominal and Pelvic Floor Muscles at Different Postures in Nulliparous Women: a Randomized&nbsp;Case Control Study
Authors: Molnár Tímea , Domján Andrea , Szűcs Mónika , Surányi Andrea , Bódis József 
Published: January 2021

Abstract
Background: To determine the effects of the pelvic floor muscle training (PFM-T) in combination with transverse abdominal muscle (TRA) activation (cPFM-T) in female urinary incontinence.Methods: We enrolled nulliparous women in supine (SUG) (n = 22), sitting (SIG) (n = 19) and control (COG) (n = 14) groups. We performed the 8-week cPFM-T programme. We examined the effect of training on the parameters with the Kruskal–Wallis test, and the pairwise comparisons with the Mann-Whitney U-test and the Wilcoxon-rank test with the Bonferroni correction.Results: Before training, 15 participants reported occasional urinary leakage. After cPFM-T seven participants reported that urinary leakage had disappeared. Maximal isometric contraction of the pelvic floor muscles (PFM) until fatigue improved significantly in the SUG (p < 0.001) and SIG (p = 0.015) and not significantly in the COG (p = 0.499). Holding time increased in the SUG (p = 0.972) and the SIG (p = 0.717), and decreased in the COG (p = 0.132). The dynamic endurance of the PFM improved significantly in the SUG (p < 0.001), but not in the SIG (p = 0.798) and the COG (p = 0.153). The number of maximal fast contractions within 1 minute increased in both the SUG (p < 0.001) and the SIG (p=0.813) and decreased in the COG (p = 0.257). Relaxation improved significantly in the SIG (p = 0.011). TRA thickness increased in both training groups.Conclusions: Slow-twitch fibres of the PFM can be trained effectively with PFM-T in both the body positions.Trial registration: This study was registered in the Hungarian National Healthcare Service Center: 019234/2014/OTIG Registered 07 April 2014 modification: 096623-002/2015/OTIG Registered 11 November 2015 https://www.aeek.huThe study was retrospectively registered in ClincalTrials.gov NCT04577872 Registered 22 September 2020. https:// https://clinicaltrials.gov
WITHDRAWN: Microbial Degradation of Ancient Wood Housed in Cheops Boat Museum and Methods of&nbsp;Its Control
Authors: Omar A M. , Abdelmoniem Abdelmoniem M. , El-Wekeel F 
Published: January 2021

Abstract
The present research paper aims to determine the effect of microbial deterioration on wood housed in Cheops Boat and how to control it. Khufu, the second king of the Fourth Dynasty 2650 BC, established Cheops Boat that was found in 1954 on the southern side of the pyramid. Wood is usually very sensitive to biological attacks. Therefore, fungi, bacteria, and insects can easily attack and metabolize it, leading to significant physical, chemical, and morphological changes. Cellulose agar was used for the cultivation of fungi, and nutrient agar was used to cultivate bacteria. Microbial infections were identified using light microscopy and PCR after their cultivation. The causative microorganisms were isolated from Cheops Boat and characterized as A. niger, A. flavus, A. sulphureus, P. janthinellum, Cladosporium herbarum, Botryotrichum piluliferum, and Bacillus megaterium. The characteristics of the test methods and disinfection include their application to historical objects. Historic wood was analyzed from different perspectives: Stereo microscopes, SEM with EDX, FTIR, as well as wood structure and chemical composition. The biological activities of the isolated microorganisms were studied. The results illustrated that the best concentrations of a specific microbicide for the bio-treatment of the infected wood materials is pentachlorophenol at (900 ppm) as it is sufficient to inhibit all isolated microorganisms. It is followed by plant extract of Ceratophyllum demersum at (1000 ppm) concentration, and p-chloro-m-cresol at (2000 ppm) concentration.