bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of CDCA7&nbsp;Is a Prognostic Biomarker and Potential Therapeutic Target in Non-small Cell Lung Cancer&nbsp;
Authors: yuan wen , Zeng Wenhui , Tan Haiyan , Jamal Muhammad , Xie Tian , Li Jingyuan , Song Henya , Jang Wanli , Huang Jie , Zhang Qiuping , Xie Songping 
Published: February 2021

Abstract
BackgroundCell Division Cycle Associated 7 (CDCA7) was first identified as a direct target gene of c-Myc and dysregulated in various types of human cancer. However, it has limited implication in non-small Cell Lung Cancer (NSCLC). We aimed to explore the critical role of CDCA7 in NSCLC.Methods In this study, we identified CDCA7 upregulation and association with the prognosis of NSCLC by integrating analysis of 3 Gene Expression Omnibus (GEO) databases. Real-time PCR and immunohistochemistry (IHC) were used to determine collected clinical NSCLC samples. Chi-square test was used to examine possible correlations between CDCA7 expression and clinicopathological factors. Univariate and multivariate Cox proportional hazards regression analysis were performed to determine whether CDCA7 is an independent risk factor for overall survival (OS). The effect of CDCA7 expression on proliferation, cell cycle and apoptosis ability of NSCLC cells was detected by cell counting kit-8 (CCK-8) and flow cytometry. CDCA7 stably knocking down cell line was established and Western blotting assay was applied to measure relevant protein expression. Xenograft models were used to examine the role of CDCA7 on tumorigenicity of NSCLC cells.Results Analysis of clinical samples confirmed the CDCA7 high expression in tumor tissues compared with adjacent non-tumor tissues and predicted shorter OS time. COX proportional risk model analysis showed that the expression levels of CDCA7 was independent prognostic factors. We observed that CDCA7 silencing efficiently affect the proliferation, apoptosis and cycle distribution of NSCLC cells in vitro. Further results demonstrated that the expression of CDCA7 in A549/DDP cells was significantly higher than that in A549 cells, CDCA7 silencing efficiently down regulation cisplatin sensitivity in A549/DDP cells. Importantly, the depletion of CDCA7 strongly reduced the tumorigenicity of NSCLC cells in vivo. Furthermore, depletion of CDCA7 expression markedly affected the expression of cell division protein kinase 6 (CDK6) and caspase7 both in vitro and in vivo. In vitro study, we showed that CDCA7 silencing promotes A549 apoptosis via extracellular regulated protein kinases (ERK) pathway.ConclusionHighly expressed CDCA7 plays a crucial role in the pathogenesis of NSCLC and might be a potential prognostic factor and therapeutic target in NSCLC.
Surgery of pancreas tumors in pediatric and adolescent patients: a single institution experience in South America
Authors: TORRES OSCAR RICARDO PAREDES , Kawaguchi Yoshikuni , Figueroa Eloy Ruiz , Meza Eduardo Payet , Espinoza Francisco Berrospi 
Published: February 2021

Abstract
Purpose
Pancreas tumors are extremely rare in pediatric and adolescent patients. Surgical resection is the mainstay of treatment; however, the data is limited with respect to morbidity and mortality. We aimed to evaluate short- and long-term outcomes of pediatric and adolescent patients who underwent surgical resection of pancreatic tumors.
Methods
Patients \(\le\) 18 years old who underwent resection of pancreas tumor at the National Institute of Neoplastic Diseases INEN during 2000–2020 were included.
Results
Thirty-four patients were diagnosed; 28 patients were female and 6 were male. The median age was 13.4 years old. Histological diagnosis was solid pseudopapillary neoplasm (SPN) (n = 29,85.3%), pancreatoblastoma (n = 3), neuroendocrine carcinoma (n = 1), and insulinoma (n = 1). No patient experienced postoperative mortality and 15 (44.1%) patients developed postoperative complications including pancreatic fistula as the most frequent. Under a median follow-up period of 33.8 (0.5–138) months, 4 (11.8%) patients died. Of the 29 patients with SPN, the 3-and-5-year OS was 100% and 83.1%, respectively.
Conclusions
SPN was the most frequent cause of surgical treatment for pediatric and adolescent patients in the high-volume cancer center in Peru and was associated with favorable survival. Pancreaticoduodenectomy was safely performed in this patient group with acceptable morbidity and zero mortality.
Feasibility of Accelerated 3D T1-Weighted MRI using Compressed-SENSE: Application to Quantitative Volume Measurement of Human Brain Structures
Authors: Yarach Uten , Saekho Suwit , Setsompop Kawin , Suwannasak Atita , Boonsuth Ratthaporn , Wantanajittikul Kittichai , Angkurawaranon Salita , Angkurawaranon Chaisiri , Sangpin Prapatsorn 
Published: February 2021

Abstract
Objective

Scan time reduction is crucial for volumetric acquisitions to improve workflow productivity and to reduce motion artifact during MRI procedure. We find out whether Compressed SENSE-4 (CS-4) can be pushed with 3D-turbo-field-echo T1-weighted (3D-TFE-T1W) sequence without compromising subcortical measurements on clinical 1.5T MRI.
Materials and methods

Thirty-three healthy volunteers (24 females, 9 males) underwent 1.5T MRI equipped with 12-channel coil. 3D-TFE-T1W for whole brain coverage was performed with different acceleration factors including SENSE-2, SENSE-4, CS-4. Freesurfer, FSL’s FIRST, and volBrain packages were implemented for subcortical segmentations. All processed data were assessed using Wilcoxon signed-rank test. A p-value < 0.05 was considered statistically significant.
Results

In comparison with reference, signal-to-noise ratio (SNR) obtained by CS-4 slightly dropped, in which the maximum SNR drop was detected at Amygdala with value of 10.55%. However, such disadvantage did not affect volume measurements - there were no statically significant differences for all subcortical structures (p < 0.05). They were in strong correlation, in which Pearson’s correlation coefficients were larger than 0.90. Moreover, the effect size scores are mostly in trivial to small ranges with values of 0.07–0.23.
Conclusion

CS-4 provided sufficient quality of 3D-TFE-T1W images for 1.5T MRI equipped with 12-channel receive coil. Subcortical volumes obtained from CS-4 images were consistent across different post-processing packages.
The efficacy and mechanism of platelet-rich plasma on the myogenic differentiation process of adipose-derived stem cell: A bioinformatics and experimental study
Authors: Wang Xiaochen , Fei Wenyong , Liu Mingsheng , Shen Fanglin , Liu Yu , Gu sanjun , Wei Changbao , Yin Qin 
Published: February 2021

Abstract
Background: The adipose-derived stem cells (ADSCs) have been proved to be effective in the myogenic differentiation with the 5-Aza induction. However, the induction procedure definitly decreased the proliferation ability of ADSCs. The platelet-rich plasma (PRP), famous of its proliferating abilities, is widely used in the field of tissue regeneration. The purpose of this study is to determine the efficacy and mechanism of PRP on the myogenic differentiation process.Method: The published gene expression profile, GSE 70918, which compared the efficacy of PRP and platelet-rich plasma (PPP) on the tendon fibroblast was obtained from the Gene Expression Omnibus (GEO) database.The further protein‑protein interaction (PPI) network Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed based on differentially epressed genes(DEGs). Furethermore, the micro-RNA-mRNA interaction network was analyzed and visualized by cytoscape. Finally, the in vitro experimental cell research was performed to certify the efficay of PRP on myogenic differentiation.Results: From our analysis, 148 DEGs, 87 up-regulated and 61 down-regulated, were obtained from the gene profile. The PPI network showed that the inflammatory factors as IL-1A, CXCL, CXCR etc. were significantly down-regulated and the muscle functional related genes as MYLC and MYO7A were significantly up-regulated. The GO and KEGG analysis demonstrated that up-regulated DEGs were enriched in focal adhesion, dilated cardiomyopathy, hypertrophic cardiomyopathy and calcium signaling pathway which were correlated to muscle function. Meanwhile, the microRNA-mRNA interaction network were estabilished based on DEGs. The in vitro experimental showed that PRP can significantly increase the proliferation ability of ADSCs during myogenic introduction and increase the expression of troponin and myosin which were correlate to the function of mature muscle.Conclusions:The research showed that PRP is an effective way to enhance the proliferation ability during the myogenic differentiation and increase the expression of muscle functional related protein.
Robust topological edge states and superconductivity in few-layer stanene
Authors: Zhao Chenxiao , Li Leiqiang , Zhang Liying , Qin Jin , Ma Haiyang , Xia Bing , Yang Bo , Zheng Hao , Wang Shiyong , Liu Canhua , Li Yaoyi , Guan Dandan , Cui Ping , Zhang Zhenyu , Jia Jinfeng 
Published: February 2021

Abstract
Stanene was proposed to be a large-gap quantum spin Hall insulator, yet to date, convincing evidence of topological edge states in stanene remains to be seen, partly due to the fact that the topological property depends on the interplay between substrate, chemical functionalization, and layer thickness. Here we fabricate 1-5 layer high-quality stanene films on the Bi(111) substrate by using hydrogen atoms as surfactants, and demonstrate their strikingly robust nontrivial topology using scanning tunneling microscopy/spectroscopy and first-principles calculations. The observed topological edge states possess a bilateral-penetration depth shorter than 4 nm, allowing the formation of dense and parallel multi-edge-channels. Our
calculations further show that surface hydrogenation helps to improve the quality of stanene films, while the Bi substrate endows the films with robust nontrivial topology. These stanene films also exhibit superconductivity, and the coexistence of
nontrivial topology and superconductivity renders them distinct potential to become the simplest one-dimensional topological superconductors.
Petasin and Isopetasin Reduce CGRP Release from Trigeminal Afferents Indicating an Inhibitory Effect on TRPA1 and TRPV1 Receptor Channels
Authors: Kleeberg-Hartmann Johanna , Vogler Birgit , Messlinger Karl 
Published: February 2021

Abstract
Background: Butterbur root extract with its active ingredients petasin and isopetasin has been used in the prophylactic treatment of migraine for years, while its sites of action are not completely clear. Calcitonin gene-related peptide (CGRP) is known as a biomarker and promoting factor of migraine. We set out to investigate the impact of petasins on the CGRP release from trigeminal afferents induced by activation of the calcium conducting transient receptor potential channels (TRPs) of the subtypes TRPA1 and TRPV1.Methods: We used well-established in vitro preparations, the hemisected rodent skull and dissected trigeminal ganglia, to examine the CGRP release from rat and mouse cranial dura mater and trigeminal ganglion neurons, respectively, after pre-incubation with petasin and isopetasin. Mustard oil and capsaicin were used to stimulate TRPA1 and TRPV1 receptor channels. CGRP concentrations were measured with a CGRP enzyme immunoassay. Results: Pre-incubation with either petasin or isopetasin reduced mustard oil- and capsaicin-evoked CGRP release compared to vehicle in an approximately dose-dependent manner. These results were validated by additional experiments with mice expressing functionally deleted TRPA1 or TRPV1 receptor channels.Conclusions: Earlier findings of TRPA1 receptor channels being involved in the site of action of petasin and isopetasin are confirmed. Furthermore, we suggest an important inhibitory effect on TRPV1 receptor channels and assume a cooperative action between the two TRP receptors. These mechanisms may contribute to the migraine prophylactic effect of petasins.
Change in Postprandial Level of Remnant Cholesterol After a Daily Breakfast in Chinese Patients With Hypertension
Authors: Xu Jin , Qu Peiliu , Du Xiao , Xiang Qunyan , Guo Liling , Zhu Liyuan , Tan Yangrong , Fu Yan , Wen Tie , Liu Ling 
Published: February 2021

Abstract
Background: Hypertension(HBP) is usually accompanied by hypertriglyceridemia that represents the increased triglyceride-rich lipoproteins(TRLs) and their cholesterol content(i.e. remnant cholesterol, RC). According to the European Atherosclerosis Society(EAS), high RC(HRC) is defined as fasting RC ≥ 0.8mmol/L or/and postprandial RC ≥ 0.9mmol/L. However, little was known about postprandial change in RC level after a daily meal in Chinese patients with HBP. Methods: One hundred and thirty-five subjects, including 90 hypertensive patients(HBP group) and 45 non-HBP controls(CON group), were recruited in this study. Serum levels of blood lipids, including calculated RC, were explored at 0, 2, and 4 h after a daily breakfast. Receiver operating characteristic(ROC) curve analysis was used to determine the cut-off point of postprandial HRC.Results: TG and RC levels increased significantly after a daily meal in two groups(P<0.05). However, postprandial RC level was significantly higher in HBP group( P<0.05). ROC curve analysis showed that the optimal cut-off point for RC after a daily meal to predict HRC in corresponding to fasting RC 0.8 mmol/L was 0.91 mmol/L, which was very close to that recommended by the EAS, i.e. 0.9mmol/L. Fasting HRC was found in 31.1% hypertensive patients but not in the controls. According to the postprandial cut-off point, postprandial HRC was found in about half of hypertensive patients and about one third of controls. Conclusion: Postprandial RC level increased significantly after a daily meal and hypertensive patients had higher percentage of HRC. More importantly, the detection of postprandial lipids is helpful to find HRC.
Effect of ABO Blood Groups, Age and Gender on Coagulation Assays in Patients With Acute Deep Vein Thrombosis
Authors: Chen Zeliang , Deng Jinhua , Hu Qingna , Cao Jing , Wu Lishan , Tan Xuerui , Yu Min 
Published: February 2021

Abstract
Background: Genetic polymorphisms, age, gender and body weight are major determinants of warfarin dose requirements. Our previous study showed that patients with non-O blood groups required higher doses of warfarin than those with O blood groups. However, the underling mechanism was not well known.Objectives: The study aimed to investigate the influence of ABO blood group, age and gender on the values of coagulation tests in patients with acute deep vein thrombosis in Chinese Han population.Methods: A retrospective study was conducted in the First Affiliated Hospital of Shantou University Medical College in Southern China. Two hundreds and 35 patients with confirmed diagnosis of acute deep vein thrombosis were included. Coagulation tests were determined.Results: Of 235 patients with acute deep vein thrombosis of lower extremities, 70 patients had blood group A (29.79%), 71 patients had blood group B (30.21%), 30 patients had blood group AB (12.77%), and 64 patients had blood group O (27.23%). The study showed no difference about coagulation tests among the patients with different blood groups. Coagulation tests were not affected by age and gender.Conclusion: Our study showed that ABO blood group, age and gender had no effects on coagulation tests in patients with acute deep vein thrombosis
Assessing biotic and abiotic interactions of micro-organisms in Amazonia through co-occurrence networks and DNA metabarcoding
Authors: Ritter Camila DUARTE Duarte , Forster Dominik , Azevedo Josue A. R. , Antonelli Alexandre , Nilsson R. Henrik , Trujillo Martha E. , Dunthorn Micah 
Published: February 2021

Abstract
Species may co-occur due to responses to similar environmental conditions, biological associations, or simply because of coincident geographical distributions. Disentangling patterns of co-occurrence and potential biotic and abiotic interactions is crucial to understand ecosystem function. Here we used DNA metabarcoding data from litter and mineral soils collected from a longitudinal transect in Amazonia to explore patterns of co-occurrence. We compared data from different Amazonian habitat types, each with a characteristic biota and environmental conditions. These included non-flooded rainforests (terra-firme), forests seasonally flooded by fertile white waters (várzeas) or by unfertile black waters (igapós), and open areas associated with white sand soil (campinas). We ran co-occurrence network analyses based on null models and Spearman correlation for all samples and for each habitat separately. We found that one third of all operational taxonomic units (OTUs) were bacteria and two thirds were eukaryotes. The resulting networks were nevertheless mostly composed of bacteria, with fewer fungi, protists, and metazoans. Considering the functional traits of the OTUs, there is a combination of metabolism modes including respiration and fermentation for bacteria, and a high frequency of saprotrophic fungi (those that feed on dead organic matter), indicating a high turnover of organic material. The organic carbon and base saturation indices were important in the co-occurrences in Amazonian networks, whereas several other soil properties were important for the co-exclusion. Different habitats had similar network properties with some variation in terms of modularity, probably associated with flooding pulse. We show that Amazonian micro-organism communities form highly interconnected co-occurrence and co-exclusion networks, which highlights the importance of complex biotic and abiotic interactions in explaining the outstanding biodiversity of the region.
Combined transcriptional, translational and cell surface targeted gene therapy of HER2-positive breast cancer stem cells in three-dimentional cell culture
Authors: Moradian Cobra , Rahbarizadeh Fatemeh 
Published: February 2021

Abstract
Purpose
Breast Cancer Stem Cells (BCSCs) resist conventional treatments and cause tumor recurrence. Almost 25% of breast cancers overexpress human epidermal growth factor receptor-2 (HER2). Here we developed a novel multi-targeted nanosystem to specifically eradicate HER2-positive BCSCs.
Methods
Plasmids containing CXCR1 promoter, PE38 toxin and 5′UTR of the basic fibroblast growth factor-2 (bFGF 5'UTR) were constructed. Polyamidoamine (PAMAM) dendrimers functionalized with an anti-HER2 VHH were used for plasmid delivery. Stem proportion of MCF-10A and MDA-MB-231/HER2+ (a cellular model of HER2 overexpression, developed in our lab) were evaluated by mammosphere formation assay. Hanging drop technique was used to produce spheroids. The uptake, gene expression and killing efficacy of the multi-targeted nanosystem were evaluated in both monolayer and spheroid culture.
Results
MDA-MB-231/HER2+ had more than 3 times higher ability to form mammosphere compared to MCF-10A, representing higher stem proportion in MDA-MB-231/HER2+. In hanging drop culture, MDA-MB-231/HER+ generated compact well-rounded spheroids, while MCF-10A failed to form compact cellular masses. The multi-targeted nanosystem showed much better uptake, higher PE38 expression and subsequent cell death in MDA-MB-231/HER2+ compared to MCF-10A. However efficacy of our targeted toxin gene therapy were lower in MDA-MB-231/HER2+ spheroid than those in monolayer.
Conclusions
Our novel multi-targeted nanosystem resulted in synergistic cytotoxic effects in HER2-positive BCSCs. This results demonstrate the necessity to use a combinatorial tumor targeting approach, to restrict the expression of a killer gene to target cells. Lower efficacy of our targeted gene therapy in spheroid models vs. monolayer culture suggest that, anticancer therapy assessments using spheroid might be more predictive of clinical efficacy than conventional monolayer culture.
Clinical Value of Optic Nerve Sheath Diameter Assessment in Prognosis of Comatose Patients with Supratentorial Lesions
Authors: Zhu Sha , Cheng Chao , Zhao Dianjiang , Zhao Yuanli , Liu Xianzeng , Zhang Jun 
Published: February 2021

Abstract
Background and objectiveOptic nerve sheath diameter (ONSD) and ONSD/ eyeball transverse diameter (ETD) ratio have been proved to be related to intracranial pressure. This study aimed to evaluate ONSD and ONSD/ETD ratio in comatose patients with supratentorial lesions and determine the relationship of these two indexes with the prognosis of these patients.MethodsA total of 54 comatose patients with supratentorial lesion and 50 cases of normal controls were retrospectively included in this study. ONSD and ETD was messured on un-enhanced computed tomography(CT). The difference of ONSD, ONSD/ETD ratio between the two groups was compared. The prognosis of comatose patients were scored unsing Glasgow outcome scale (COS) at 3-month follow-up and classified into good (score ≥ 3) and poor (score < 3) prognosis. The differences of ONSD and ONSD/ETD ratio between good and poor prognosis of comatose patients were compared statistically.ResultONSD and ONSD / EDT in the comatose group were 6.30 ± 0.60 mm and 0.27 ± 0.03, respectively, both were significantly greater than that in normal controls (5.10 ± 0.47 mm, t = 11.426, P < 0.0001 ; 0.22 ± 0.02, t = 11.468, P < 0.0001 ; respectively). ONSD in poor prognosis was siginificantly greater than that in good prognosis (6.40 ± 0.56 mm vs. 6.03 ± 0.61 mm, t = 2.197, P = 0.032). ONSD / EDT raito in poor grognosis was significantly higher than that in good gronosis (0.28 ± 0.02 vs 0.26 ± 0.03, t = 2.622, P = 0.011). The area under the responder operating charateristic curve to predict prognosis of comatose patients were 0.650 ( 95% CI: 0.486–0.815, P = 0.078) for ONSD and 0.711( 95% CI: 0.548–0.874, P = 0.014) for ONSD/ETD ratio, respectively.ConclusionsONSD and ONSD / EDT raito increased in comatose patients. ONSD / EDT raito may be more valuable than ONSD in evaluation for prognosis of comatose patients with Supratentorial lesions.
WITHDRAWN: Ethiopia’s Antibiotic Footprint: Estimate of National Antibiotic Consumption Using 2018 Data
Authors: Gutema Girma , Ali Seid , Engidawork Ephrem , Kaba Mirgissa , Toverud Else-Lydia 
Published: February 2021

Abstract
The processes involving resistance development against antibiotics have historically been part of the Darwinian evolution. But in the backdrop of this evolutionary reality, the increasing use of antibiotics in modern medicine, particularly in post-penicillin discovery era, has intensified the selection pressures with an acute gear up rather than as part of the so slow and natural evolutionary process that selects for enhanced fitness for survival. Two major recommendations were made in the past years to tackle this challenge: 1) incentivizing the pharmaceutical industry to invest more on research and development endeavors, so that they come up with new and novel antibiotics; 2) implementing antimicrobial stewardship programs in healthcare systems. In the current study, the third and the emerging approach — the idea of antibiotic footprint — was employed as a communication tool that targets individual consumers of antibiotics to tackle the problems of their misuse and overuse. A total of 698.2 tons of antibiotics were used in Ethiopia in 2018, and the per capita consumption of antibiotics was 5.8 grams per person. Penicillins with extended spectrum (J01CA) and beta-lactamase-resistant penicillins (J01CF) were the most commonly utilized classes of antibiotics which accounted for, respectively, 38.3% and 20.8% of all antibiotics used in the country’s public health sector. Hubs in Addis Ababa (14%) and Hawassa (12%) topped the overall antibiotic consumptions in the country. Contrarily, hubs in Gambella and Semera received relatively smaller quantities of antibiotics, with totals of 4.8 tons (0.9%) and 10.2 tons (1.9%), respectively.