bluuebunny/crossref_metadata_2025_split huggingface dataset:
SOSIALISASI PROGRAM JAMINAN KESEHATAN NASIONAL (JKN) DALAM MENINGKATKAN PARTISIPASI KEPESERTAAN MANDIRI BPJS KESEHATAN DI DESA SUNGAI BELIDAK KECAMATAN SUNGAI KAKAP KABUPATEN KUBU RAYA
Authors: Maryuni Sri , Eka Agus , - Pardi 
Published: April 2021

<p>Badan Penyelenggara Jaminan Sosial (BPJS) Kesehatan merupakan sebuah badan yang dibentuk oleh pemerintah untuk melaksanakan Program Jaminan Kesehatan Nasional. Jaminan Kesehatan Nasional (JKN) yang dikembangkan di Indonesia merupakan bagian dari Sistem Jaminan Sosial Nasional (SJSN). Tujuannya adalah agar semua penduduk Indonesia terlindungi dalam sistem asuransi, sehingga terpenuhinya kebutuhan dasar kesehatan yang layak.<strong> </strong>Konsep yang digunakan oleh BPJS Kesehatan dalam memenuhi kebutuhan dasar kesehatan masyarakat dengan menggunakan sistem gotong-royong. Dalam SJSN, prinsip gotong royong berarti peserta yang mampu membantu peserta yang kurang mampu, peserta yang sehat membantu yang sakit atau yang berisiko tinggi, dan peserta yang sehat membantu yang sakit. Dalam kegiatan inilah, peneliti berusaha untuk memperkenalkan BPJS Kesehatan beserta program JKN-KIS kepada masyarakat, khususnya masyarakat Desa Sungai Belidak Kecamatan Sungai Kakap Kabupaten Kubu Raya untuk menjelaskan tentang manfaat dan pentingnya menjadi peserta JKN-KIS.<strong> </strong>Metode yang digunakan dalam pelaksanaan kegiatan PKM ini adalah dengan melakukan survei lapangan dan sosialisasi. Berdasarkan hasil dari kegiatan ini ditemukan fakta bahwa sebelum sosialisasi dilakukan, masih banyaknya masyarakat yang memiliki pengetahuan dan kesadaran yang masih terbatas terhadap pentingnya menjadi kepesertaan JKN-KIS.<strong></strong></p>
Implikasi Kewenangan Kecamatan dalam Penyelenggaraan Urusan Pemerintahan Umum (Studi Kasus Di Kecamatan Pandeglang Kabupaten Pandeglang)
Authors: Nugroho Arif 
Published: April 2021

<p>Penelitian ini membahas tentang implikasi kewenangan kecamatan dalam penyelenggaraan urusan pemerintahan umum. Pendekatan penelitian yang digunakan adalah deskriptif kualitatif. Teknik pengumpulan data menggunakan wawancara, dokumentasi dan telaah literatur dengan menggunakan teknik penentuan informan yaitu purposive sampling. Hasil penelitian menunjukan bahwa dalam penyelenggaraan pemerintahan umum kecamatan belum cukup tertopang oleh elemen <em>necessary condition</em> diantaranya kepastian atas kewenangan legalnya serta Anggaran. Maka dari pada itu perlu mendorong stakeholder kebijakan untuk menetapkan regulasi yang dapat memberikan optimalisasi pada implikasi kewenangan kecamatan dalam penyelenggaraan pemerintahan umum di Kecamatan Pandeglang Kabupaten Pandeglang.</p><p><span style="text-decoration: underline;"> </span></p><p>Kata Kunci : Pemerintahan Umum, <em>decentralization within cities</em></p><p><strong> </strong></p>
Associations of residential greenness with hypertension and blood pressure in a Chinese rural population: A cross-sectional study
Authors: Jiang Jie , Chen Gongbo , Li Baojing , Li Na , Liu Feifei , Lu Yuanan , Guo Yuming , Li Shanshan , Chen Lifeng , Xiang Hao 
Published: April 2021

Abstract
Background: Limited epidemiological literature identified the associations between residential greenness and hypertension in low/middle-income countries.Methods: A random sampling strategy was adopted to recruit 39 259 residents, ≥ 18 years, and from 5 counties in central China. Blood pressure was measured based on the protocol of the American Heart Association. Hypertension was defined according to the 2010 Chinese guidelines for the management of hypertension. The satellite-derived Normalized Diﬀerence Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) were applied to estimate the residential greenness. Mixed logit model and mixed linear model were utilized to explore the relationships of residential greenness with hypertension and blood pressure.Results: High residential greenness was associated with lower odds of hypertension and blood pressure levels. For instance, an interquartile range (IQR) increase in NDVI500m was linked with lower odds of hypertension (OR = 0.92, 95%CI 0.88 to 0.95), a decrease of -0.88 mm Hg (95%CI -1.17 to -0.58) and -0.64 mm Hg (95%CI -0.82 to -0.46) in SBP and DBP, respectively. The effect of residential greenness was more pronounced in males, smokers, and drinkers.Conclusions: Long-term exposure to residential greenness may reduce the risk of hypertension. More prospective studies are needed to verify the hypothesis.
Extracellular vesicle formation mediated by local phosphatidylserine exposure promotes efficient cell extrusion
Authors: Kira Akihito , Murata Machiko , Saito Keisuke , Tatsutomi Ichiko , Hattori Izumi , Kajita Haruna , Muraki Naoko , Oda Yukako , Satoh Saya , Tsukamoto Yuta , Kimura Seisuke , Kato Hiroki , Kawane Kohki 
Published: April 2021

Abstract
Numerous unwanted cells are removed from epithelial and endothelial tissues—in which cells are tightly connected to one another—without disturbing tissue integrity and homeostasis. Cell extrusion is a unique mode of cell removal from tissues, and it plays an important role in regulating cell numbers and the eliminating unwanted cells, such as apoptotic cells, cancer cells, and cells with a lower fitness in cell competition . During this process, cells delaminate from the cell layer, to which they initially used to adhere, through communication with the neighboring cells. Defects in cell extrusion are believed to associate with inflammation and cancer in epithelium as well as blood vessel dysfunction. However, the correlation between them has not yet been evaluated owing to a lack of knowledge of the underlying mechanisms. In particular, the process whereby the cell exit from the tissue remains to be elucidated. Here, we report a novel and conserved execution mechanism of cell extrusion—common to mammalian cells and Drosophila epithelia—i.e., spatiotemporally regulated extracellular vesicle formation in extruding cells at a site opposite to the direction of extrusion. Particularly, we found that a lipid-scramblase‒mediated local exposure of phosphatidylserine is responsible for extracellular vesicle formation and is crucial for the execution of cell extrusion, and inhibition of this process disrupted prompt cell delamination as well as tissue homeostasis. Furthermore, we revealed the mechanism underlying vesicle formation. Importantly, our results reveal that membrane dynamics is the driving force by a “rocket launch”-like mechanism behind the extrusion of cells from tissues, a fundamental cell behavior in multicellular organisms that is also observed in other contexts including cancer cell invasion and neural cell differentiation. Our understanding of this new mechanism of cell extrusion enables us to examine the relationship between cell extrusion abnormalities and the onset of various diseases.
Determination of in Vivo Biological Activities of Dodonaea Viscosa Flowers Against CCL4&nbsp;Toxicity in Albino Mice With Bioactive Compound Detection
Authors: Gul Hina , Awais Muhammad , Saddick Salina , Khan Falak Sher , Gulfraz Muhammad , Nazir Khizar , Khan Sami Ullah , Khan M. Ijaz 
Published: April 2021

Abstract
Dodonaea viscosa L. Jacq. is an evergreen shrub and native to Asia, Africa and Australia. It has been used as traditional medicine in different countries. The foremost objective of the current study was to discover protective potential of D. Viscosa flowers Methanol (DVM) and Chloroform (DVC) extracts against CCL4 induced toxicity in mice. This study was intended to identify phytochemicals through HPLC, GCMS and FT-IR as well as in vitro antioxidant and in vitro antituberculosis activity. Our comprehensive findings indicate that Dodonaea viscosa is valuable and widespread herbal medicines through therapeutic potentials for curing various ailments. Dodonaea viscosa flowers are found to have protective effect against oxidative stress produced by CCL4 in liver, kidney and spleen. The level of hepatic enzymes (ALP, AST ALT and Direct bilirubin), hematological parameters (RBCs, WBCs and Platelets), total protein and liver antioxidant enzymes (SOD, GPx and CAT) were restored by the intake of DV extracts after decline in levels by CCL4. Histopathological results discovered the defensive effect of 300mg/kg of DVM extract against CCL4 induced damage, thus having improved protective effect as compared to DVC and control. As a result of analysis total flavonoids and total phenolics were also revealed. Phytochemical investigation by HPLC identified gallic acid, epicatechin, cumeric acid, flavonoids while Oleic acid (Octadecenoic acid) (C18H34O2), Stearic acid (C18H36O2), Ricinoleic acid (C18H34O3) and Cedrol (C15H26O) was estimated by GCMS. DVM extract exhibited resistance against in vitro Mycobacterium tuberculosis strains. This study proposed that protective effect of DV against oxidative damage induced in Liver, Kidney and Spleen can possibly be correlated to their antioxidant as well as free radical scavenging property.
A Comparative Study of Spiritual Needs of Thai Terminal Ill Cancer Patients
Authors: Wisesrith Wasinee , Sukcharoen Pilaiporn , Sripink Kanittha 
Published: April 2021

Abstract
Purpose Thai terminal ill cancer patients have spiritual distress and need spiritual care. They are in need of palliative care in the long term to alleviate suffering, achieve good spirituality, and to be ready to face with terminal ill conditions. This study aimed to investigate spiritual needs and compare differences in spiritual needs. Methods A quantitative design was used for data collection and data analysis. The participants were Thai terminal ill cancer patients from Thai seven hospitals in northern, northeast, central, and southern regions; 322 participants were purposively selected. Instruments used was a spiritual needs scale. The instrument’s validity was validated by 17 experts, a Delphi technique was performed, the Cronbach’s alpha of 0.89 was found. Statistical analysis was F-test for one-way analysis of variance. Results The overall spiritual needs of terminal ill cancer patients were at the moderate level. The highest mean was found in the ‘prepare for death’ dimension, followed by ‘have meaning, values, and life purposes’ and ‘have opportunity to pursue most important things in life’ dimensions consecutively and had different spiritual needs among status, number of family members, living with family, and spiritual anchor at the statistical significance level of .05. Conclusion: The study results provide insight into spiritual needs of terminal ill cancer patients. That assists in improving the provision of holistic care to let the patients be happy at end-of-life phase.
De novo Transcriptome and tissue specific expression analysis of gene associated with biosynthesis of medicinally active metabolites in a high valued medicinal plant Operculina turpethum (L.)
Authors: Biswal Bhagyashree , Jena Biswajit , Giri Alok Kumar , Acharya Laxmikanta 
Published: April 2021

Abstract
Operculina turpethum (L.) Silva Manso (Nisoth), an important medicinal plant whose root and stem tissues are found to be key ingredient in more than 135 herbal formulation in both Unani and Ayurvedic medicine system and is used for the treatment of various health disorders. It has a very high demand in the Indian Pharmaceutical industry with an annual average consumption of about 660 metric tonne (dry weight). The plant because of its wide usability is also exported to countries like Sri Lanka, Singapore, Netherlands, Japan, United States, etc. leading to a total export value of approximately 2.4 million USD. Terpenoids, steroid glycosides, resin glycosides, dammarane type triterpenoid saponins, flavonoids, coumarins are the major bioactive principles present in O. turpethum. However, overexploitation of root/root bark over the other part of the plant has caused depletion of its germplasm resources from the wild while bringing it to Near Threatened category which subsequently lead to unavailability of the requisite plant material followed by adulteration. Additionally, the limited genomics and transcriptomic resource is a major hindrance in the genetic and molecular research including elucidation of biosynthetic pathway, identification of enhanced traits and proper authentication and genetic diversity study of the plant. Hence, de novo transcriptome sequencing of root and stem tissues of O. turpethum was performed using Illumina HiSeq platform which generated a total of 64259 unigenes and 20870 CDS (coding sequence) with a mean length of 449bp and 571bp respectively. Further, 20218 and 16458 unigenes showed significant similarity with the identified proteins of NR(non-redundant) and Uniprot database respectively. The homology search carried out against publicly available database found the best match with Ipomoea nil sequences (82.6%). The KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis identified 6538 unigenes functionally assigned to 378 modules with phenylpropanoid biosynthesis pathway as the most enriched among the secondary metabolite biosynthesis pathway followed by terpenoid biosynthesis. Moreover, transcription factors, SSRs (Simple sequence repeats) and SNPs (single nucleotide polymorphism) were also identified in this study. Apparently, the present investigation reported the first ever transcriptome analysis of the root and stem tissues of the non-model plant O. turpethum of family Convolvulaceae, which will provide a baseline for further molecular research.
Clinical Impact of PD-L1 Expression in Triple Negative Breast Cancer Patients With Residual Tumor Burden After Neoadjuvant Chemotherapy
Authors: Oner Gizem , Onder Semen , Karatay Hüseyin , Ak Naziye , Tukenmez Mustafa , Muslumanoglu Mahmut , Igci Abdullah , Dinccag Ahmet , Ozmen Vahit , Aydiner Adnan , Yavuz Ekrem , Cabioglu Neslihan 
Published: April 2021

Abstract
Background: Studies on PD-L1 expression in breast cancer have gained importance in recent years, especially in triple negative breast cancer (TNBC). Our aim was to analyse the differential expression of PD-L1 to explore its correlation with response to neoadjuvant chemotherapy (NACT) and patient survival. Methods: PD-L1 expression was evaluated immunohistochemically (Ventana SP263 clone kit) by staining tumor specimen. PD-L1 positivity was defined as membranous staining >1%, >5%, >10% and >20% on either tumor cell (TC) and /or immune cell (IC). Results: Fifty patients with locally advanced TNBC, who had a partial response to NACT, were included in the study. Twenty-nine patients (58%) were detected to be positive for PD-L1 on both TCs and ICs, whereas 25 patients (50%) were positive for TC PD-L1 expression, and PD-L1 on ICs was detected (44%) in 23 patients. Patients with PD-L1 positivity on ICs were more likely to respond to chemotherapy as measured by “MD Anderson Cancer Center Residual Cancer Burden Index” (14/22, 63.6%, versus 10/27, 37%, p = 0.064). The 5-year disease-free survival (DFS) and disease-specific survival (DSS) rates were 46.3% and 51.4%, respectively. A high (>20%) tumoral PD-L1 positivity was associated with a better DFS and DSS. Conclusions: Our results suggest that patients with a high TC PD-L1 expression were more likely to have a better outcome. Since patients with residual TCs expressing PD-L1 on TILs were more likely to respond to NACT, an immune checkpoint inhibitor therapy in addition to NACT would be an important option for TNBC with locally advanced disease.
Treatment of Alectinib in NSCLC with Brain Metastasis Patient Refractory to Radiotherapy after Resistance to Crizotinib: A Case Report and Literature Review
Authors: ZHOU YUAN , Zeng Xianliang , Sun Songwei , Chen Xiuli , Huang Shuang , Zhang Chunzhi 
Published: April 2021

Abstract
Background: Brain metastasis is the common place of tumor recurrence after resistance to crizotinib. The therapeutic modes on brain metastasis of ALK-positive NSCLC require multidisciplinary approach, including target therapy, chemotherapy and radiotherapy. Until to nowadays, there isn`t optimal therapeutic recommendations for these patients. Radiotherapy is the vital treatment for brain metastasis.Case presentation: We reported one ALK-positive NSCLC patient with brain metastasis after crizotinib. ALK rearrangement wasn`t found in blood sample of the patient by NGS. According to NCCN guideline, we gave the patient whole brain radiotherapy. It was unexpected that the number of brain metastasis increased after whole brain radiotherapy. After that, the patient was empirically used alectinib after radiotherapy failure. It achieved unexpected success in our patient. Conclusions: We got some enlightenment form the patient. Firstly, liquid biopsy is complementary to tissue biopsy in NSCLC, mainly in EGFR mutation. However, ALK detection should use tissue biopsy as much as possible. Secondly, we suggest the brain metastasis patient of NSCLC use the second generation TKI, such as alectinib, ceritinib, after resistance to crizotinib whether ALKr is positive or negative in liquid biopsy. Lastly, we believe that combined radiotherapy with TKIs is an optimal mode in the BM patient of NSCLC after resistance to crizotinib.
Highly durable electrocatalyst with low-loading platinum-cobalt nanoparticles dispersed over single-atom Co-N-Graphene nanofiber for efficient fuel cell
Authors: Chong Lina , Wen Jianguo , Yang Zhenzhen , Lin Yulin , Bloom Ira , Abraham Daniel 
Published: April 2021

Abstract
Development of highly-active, durable and cost-effective oxygen reduction electrocatalyst for proton exchange membrane fuel cell is crucial and greatly desired to enable fuel cell powered vehicles that are competitive with internal combustion engine automobiles. The support’s structure is known to strongly influence the performance of Pt particles. Here, we present a new catalyst containing PtCo core-shell nanoparticle supported over hierarchical tailored porous carbon nanofibers with densely populated single-atomic Co-Nx sites embedded in N-doped graphene. In a fuel cell with a total Pt loading (anode + cathode) of 0.091 mg cm-2, the new catalyst delivered unprecedented mass activity of 2.28 A mgPt-1 at 0.9 ViR-free, Pt utilization of 11.1 kW gPt-1 at 150 kPaabs, and high durability with 80% retention of initial mass activity after 30,000 accelerated-stress-test cycles, significantly higher than that of the state-of-the-art Pt3Co/C. In-situ X-ray absorption spectroscopy revealed structure reversibility of the catalyst during oxygen reduction reaction and indicated that the enhanced activity can be attributed to simultaneous PtCo and Co-Nx contributions.
A novel application of temporoparietal fasciocutaneus (TPFC) and temporoparietal (TPF) pedicle flap for auricular reconstruction
Authors: Zwierz Aleksander , Masna Krystyna , Burduk Paweł 
Published: April 2021

Abstract
Background: Temporoparietal fasciocutaneus (TPFC) and temporoparietal (TPF) pedicle flaps play an important role among many techniques for auricular reconstruction. Because of their unique characteristics, the flaps based on the superficial temporal artery (STA) may be used for various types of reconstruction.Case series presentation: In this paper, we present several applications of the TPFC and TPF pedicle flaps in the reconstruction of defects after ear resection, including the use of the sandwich flap and preparing basis for Vistafix® system.Conclusion: Our experiences presented in this case series confirm the reconstructive potential of the flaps, also in patients in whom the Vistafix® system was used for reconstruction of the ear. In all cases described herein, the use of the pedicle flaps resulted in optimal healing and satisfactory cosmetic and functional outcomes.
Activity level and patient outcome 1 after liver surgery, a prospective study
Authors: Valdimarsson Valentinus , Holka Peter , Radberg John , Mania Jonathan , Sturesson Christian 
Published: April 2021

Abstract
Background: Perioperative measurement to enhance recovery after surgery has been introduced as a prognostic factor. The effect of surgery on activity level during and after hospital discharge has been relatively under-explored. The present study aims to measure perioperative activity for patients undergoing liver surgery as a benchmark for further interventional studies on the relationship between postoperative mobilization and outcome. Methods: In this prospective cohort study we measured activity levels for patients selected for liver surgery at a single liver surgical center. The activity level was measured before, during hospital admission, and after discharge with patients wearing pedometers. Clinical parameters and outcomes were documented. Results: Thirty-three patients were included. Median activity level was 4303 (2381- 6912), 293 (170-665), and 1250 (613-3300) steps per day preoperatively, perioperatively, and postoperatively after discharge, respectively. The activity level decreased to 38 (22-62) % after discharge compared to preoperative levels. Conclusion: This study quantified the decrease in activity level after liver surgery. Future studies could further evaluate the intervention effect of perioperative care on postoperative outcome measures.
Targeting CDK4 overcomes EMT-mediated tumor heterogeneity and therapeutic resistance in KRAS mutant lung cancer
Authors: Padhye Aparna , Konen Jessica , Rodriguez B. Leticia , Fradette Jared , Ochieng Joshua , Diao Lixia , Wang Jing , Lu Wei , Solis Luisa , Batra Harsh , Raso Maria Gabriela , Peoples Michael , Minelli Rosalba , Carugo Alessandro , Bristoe Christopher , Gibbons Don 
Published: April 2021

Abstract
Lack of sustained response to therapeutic agents in patients with K-Ras mutant lung cancer poses a major challenge and arises partly due to intratumor heterogeneity that defines phenotypically distinct tumor subpopulations. To attain better therapeutic outcomes it is important to understand the differential therapeutic sensitivities of tumor cell subsets. Epithelial-to-mesenchymal transition (EMT) is a biologic phenomenon that can alter the phenotypic state and cause transcriptional rewiring to produce distinct tumor cell populations. We utilized functional shRNA screens, in vitro and in vivo models to identify and confirm an increased dependence of mesenchymal tumor cells on CDK4 for survival, as well as a mechanism of resistance to MEK inhibitors. High ZEB1 levels in mesenchymal tumor cells repressed p21, leading to perturbed CDK4 pathway activity. Increased dependence on CDK4 rendered mesenchymal cancer cells particularly vulnerable to selective CDK4 inhibitors. Co-administration of CDK4 and MEK inhibitors in heterogeneous tumors effectively targeted different tumor subpopulations, subverting the resistance to either single agent treatment.