bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standardization and Evaluation of an Anti-ZIKV IgM ELISA Assay for the Serological Diagnosis of Zika Virus Infection
Authors: Sirikajornpan Kanittha , Suntarattiwong Piyarat , Suwanpakdee Detchvijitr , Tabprasit Sutchana , Buddhari Darunee , Thaisomboonsuk Butsaya , Klungthong Chonticha , Poolpanichupatam Yongyuth , Buathong Rome , Srikiatkhachorn Anon , Jones Anthony , Fernandez Stefan , Hunsawong Taweewun 
Published: August 2021

ABSTRACT.
Here, we describe the development of the in-house anti-Zika virus (ZIKV) IgM antibody capture ELISA (in-house ZIKV IgM ELISA) for the detection and diagnosis of acute ZIKV infections. We compared the in-house ZIKV IgM ELISA assay performance against two commercial kits, Euroimmun ZIKV IgM and InBios 2.0 ZIKV IgM ELISA. We tested the assays’ ability to detect anti-ZIKV IgM using a well-defined serum sample panel. This panel included 80 ZIKV negative samples (20 negative, 20 found to be primary dengue virus [DENV][ infections, 20 secondary DENV infections, and 20 Japanese encephalitis virus [JEV] infections) and 67 ZIKV reverse transcriptase–polymerase chain reaction–positive acute serum samples. The OD values were calculated to enzyme immunoassay (EIA) unts by comparing them to weak positive controls. The results demonstrated the high sensitivity (88.06%) and specificity (90.00%) of our in-house ZIKV IgM ELISA and its 89.12% overall percentage agreement. The kappa values were deemed to be within excellent range and comparable to the InBios ZIKV IgM ELISA. Some cross-reactivity was observed among secondary DENV and JEV samples, and to a much lower extent, among primary DENV samples. These data indicate that our in-house ZIKV IgM ELISA is a reliable assay for the detection of anti-ZIKV IgM antibodies in serum.
Epidemiology of Plasmodium falciparum Infections in a Semi-Arid Rural African Setting: Evidence of Reactive Case Detection in Northwestern Kenya
Authors: Meredith Hannah R. , Wesolowski Amy , Menya Diana , Esimit Daniel , Lokoel Gilchrist , Kipkoech Joseph , Freedman Betsy , Lokemer Samuel , Maragia James , Ambani George , Taylor Steve M. , Prudhomme-O’Meara Wendy , Obala Andrew A. 
Published: August 2021

ABSTRACT.
In northwestern Kenya, Turkana County has been historically considered unsuitable for stable malaria transmission because of its unfavorable climate and predominantly semi-nomadic population; consequently, it is overlooked during malaria control planning. However, the area is changing, with substantial development, an upsurge in travel associated with resource extraction, and more populated settlements forming. Recently, numerous malaria outbreaks have highlighted the need to characterize malaria transmission and its associated risk factors in the region to inform control strategies. Reactive case detection of confirmed malaria cases at six health facilities across central Turkana was conducted from 2018 to 2019. Infections in household members of index cases were detected by malaria rapid diagnostic tests (RDTs) and PCR tests, and they were grouped according household and individual characteristics. The relationships between putative risk factors and infection were quantified by multilevel logistic regression models. Of the 3,189 household members analyzed, 33.6% had positive RDT results and/or PCR test results. RDT-detected infections were more prevalent in children; however, PCR-detected infections were similarly prevalent across age groups. Recent travel was rarely reported and not significantly associated with infection. Bed net coverage was low and net crowding was associated with increased risks of household infections. Infections were present year-round, and fluctuations in prevalence were not associated with rainfall. These findings indicate year-round, endemic transmission with moderate population immunity. This is in stark contrast to recent estimates in this area. Therefore, further investigations to design effective intervention approaches to address malaria in this rapidly changing region and other similar settings across the Horn of Africa are warranted.
Evolução e dinâmica espacial intraurbana da COVID-19 em Montes Claros/MG, Brasil
Authors: França Iara Soares de , França Juliana Soares de 
Published: August 2021

Montes Claros, cidade média com população superior a 400 mil habitantes, é o foco principal de concentração do vírus por COVID-19 no Norte do Estado de Minas Gerais, resultado de sua centralidade política, econômica e estrutural, além do seu tamanho demográfico. Até o dia 16 de abril de 2020, registrou-se 30.573 casos e 721 óbitos. A partir disso, este artigo analisa a evolução e dinâmica espacial da Covid-19 em Montes Claros em dois momentos: 03/11/2020 e 19/04/2021. Focalizará na expansão da Covid-19 na escala da cidade, evidentemente sem desconsiderar suas nuances e articulação com outras escalas geográficas. A metodologia pautou-se em uma abordagem qualitativa com levantamento e organização de dados secundários de Covid-19 em Montes Claros, provenientes da Secretaria Municipal de Saúde, disponibilizados através de Boletins Informativos publicados diariamente. Os resultados foram sistematizados em produtos cartográficos analíticos como mapas e gráficos demonstrando a evolução temporal da pandemia. No que tange as análises sobre o comportamento geográfico do vírus, constatou-se sua elevada ampliação para todos os bairros revelando um padrão espacial altamente heterogêneo o que associa e implica no avanço do processo de desigualdade social nessa cidade média.
EL ALCANCE MATERIAL DE LA RESERVA DE JURISDICCIÓN DEL ART. 117.3 CE: LA STC 141/2020 Y LAS MEDIDAS CAUTELARES ADOPTADAS POR LA ADMINISTRACIÓN TRIBUTARIA EN SEDE JUDICIAL
Authors: MORENO GARCIA JOSE JAVIER 
Published: August 2021

Una modificación en 2012 de la Ley 58/2003, de 17 de diciembre, General Tributaria introdujo un nuevo apartado 8 al art. 81. En su virtud, la administración adquiría potestades para adoptar las medidas cautelares en el seno de un proceso penal por delitos fiscales, decisión que, aunque debe ratificar el juez, hasta ese momento le correspondía en exclusiva a los tribunales. La doctrina entendió que se trataba de un precepto inconstitucional por vulnerar el principio de reserva de jurisdicción, constitucionalmente garantizado en el art. 117.3 CE y se abrió así otro debate sobre el alcance del ámbito material específico reservado por la Constitución al poder judicial. En su STC 141/2020, una de las Salas del Alto Tribunal, al hilo de un recurso de amparo en el que no se planteaba la inconstitucionalidad de ese precepto, aprovecha la ocasión para dar su valoración sobre esa intromisión de la administración tributaria en el ámbito procesal.Tributuen Lege Orokorrari (2003ko abenduaren 17ko 58/2003) 2012an eginiko aldaketak 8. apartatu bat gehitu zuen 81. artikuluan. Hari esker, administrazioak zerga-delituengatiko zigor-arloko prozesu batean kautelazko neurriak hartzeko ahala bereganatu zuen; erabaki hori, epaileak berretsi behar badu ere, auzitegien esku baino ez zegoen ordura arte. Doktrinak ulertu zuen konstituzioaren aurkako manu bat zela, jurisdikzio-erreserbaren printzipioa urratzen zuelakoan (Espainiako Konstituzioaren 117.3 artikuluan bermatuta), eta Konstituzioak botere judizialari erreserbatutako eremu material espezifikoaren irismenari buruzko beste eztabaida bat ireki zen horrela. Goi Auzitegiaren Saletako bat, Konstituzio Auzitegiaren 141/2020 Epaian, eta manu horren konstituzio-kontrakotasuna planteatzen ez zuen babes-errekurtso bati lotuta, aukera horretaz baliatu zen Zerga Administrazioak prozesuko eremuan izandako esku-sartze horri buruzko iritzia emateko.In 2012, an amendment of Act 58/2003 of December 17th, on General Taxation introduced a new paragraph 8 to art. 81. Under that provision, the Administration can adopt interim measures within a criminal procedure related to tax offences, notwithstandig its following judicial ratification. The doctrine understood that it was an unconstitutional provision because it infringed the principle of jurisdiction reserve as constitutionally guaranteed under art. 117.3 of the Constitution and another debate began over the specific material scope reserved to the judiciary according to the Constitution. In its judgment 148/2000, one of the Constitutional Court chambers with regard to an action for the protection of fundamental rights where the unconstitutionality of the provision was not raised, took the opportunity to deliver its assessment about the interference of the Tax Administration within the procedural field.
EL RÉGIMEN JURÍDICO DE LOS MONTES PÚBLICOS Y SU INSCRIPCIÓN EN EL REGISTRO DE LA PROPIEDAD: DEBATE DOCTRINAL E INSTRUMENTOS JURÍDICOS DE PROTECCIÓN
Authors: FERNÁNDEZ-ESPINAR MUÑOZ CARLOS 
Published: August 2021

El presente trabajo tiene como objeto de estudio la protección del
Registro de la Propiedad en relación con los montes públicos y en especial los
montes de dominio público. En primer lugar, se realiza un detallado análisis histórico,
para más tarde analizar el desarrollo del debate doctrinal surgido en 1925
sobre la necesidad o no de inscripción de los montes de dominio público con el
fin de protegerlos de las usurpaciones de los particulares. A continuación, se llevan
a cabo unas consideraciones de fondo desde los principios y conceptos del
Derecho administrativo con un análisis de la doctrina académica administrativista.
La segunda parte del trabajo expone la solución adoptada y analiza los conflictos
actuales entre particulares y Administración en relación a los montes, así como la
regulación de los distintos textos legales de las relaciones entre los montes y el
Registro.
Lan honek xedetzat du Jabetza Erregistroaren babesa aztertzea
mendi publikoei eta, bereziki, jabari publikoko mendiei dagokienez. Lehenik,
azterketa historiko zehatza egin dugu, eta ondoren, 1925ean sortutako doktrinaeztabaidaren
garapena aztertu dugu; hau da, ea jabari publikoko mendiak
inskribatu behar diren edo ez partikularren usurpazioetatik babesteko. Jarraian,
funtsezko gogoeta batzuk egin ditugu administrazio-zuzenbidearen printzipio
eta kontzeptuen aldetik administrazio-jakintzako doktrina akademikoa aztertuta.
Lanaren bigarren zatian, batetik, hartutako irtenbidea azaldu dugu, eta bestetik,
partikularren eta administrazioaren artean, mendiei dagokienez, gaur egun
dauden gatazken azterketa jaso dugu, bai eta mendien eta erregistroaren arteko
harremanen legezko testuen arauketarena ere.
The object of this work is to study the protection of the Property
Registry in relation to public forests and especially those considered of public
domain. In the first place, a detailed historical analysis is carried out, to later
analyze the development of the doctrinal debate that arose in 1925 on the
need or not to inscribe the public domain forests in order to protect them from
landgrabbings by individuals.Then, some background considerations are carried
out from the principles and concepts of administrative law with an analysis of
the administrative academic doctrine. The second part of the work presents the solution adopted and analyzes the current conflicts between individuals and the
Administration in relation to the forests, as well as the regulation of the different
legal texts of the relationships between the public forests and the Registry.
ENERGIA-TRANTSIZIOA EUROPAR BATASUNEAN: OINARRIZKO ARAUKETA, HELBURUEN BETETZE-MAILA ETA EUROPAKO ITUN BERDEA GARATZEKO PROPOSAMENAK
Authors: GARCÍA LUPIOLA ASIER 
Published: August 2021

Uno de los objetivos del proceso de integración europea es el desarrollo sostenible. Tal y como se describe en el texto, la actividad desarrollada durante los últimos años por la UE para su logro se fundamenta en la transición energética. El análisis de las estrategias y normas europeas demuestran que la apuesta por las energías renovables y la eficiencia energética se ha convertido en la herramienta a la que se está recurriendo tanto para la protección del medio ambiente, como para la lucha contra el cambio climático y el impulso de la economía verde. No obstante, vistos los resultados, la consecución de los objetivos específicos parece ralentizarse, por lo que la UE debe reforzar su actividad para completar la transición energética. El desarrollo del Pacto Verde Europeo será fundamental para ello.Europako integrazio prozesuaren oinarrizko helburuetako bat garapen jasangarria da. Testuan azaltzen den bezala, Europar Batasunak helburu hori lortzeko azken urteotan aurrera eraman duen ekintza energia-trantsizioan oinarritzen da. Estrategiak eta arauak aztertuz, ondorioztatzen da energia berriztagarrien eta energia-efizientziaren sustapena bilakatu dela ingurunearen babeserako, klima- aldaketaren kontra borrokatzeko eta ekonomia berdea bultzatzeko tresna. Hala ere, emaitzak ikusita, helburu zehatzen lorpena moteltzen ari dela dirudi, beraz, Europar Batasunak energia-trantsizioa burutzeko aurrera eramaten duen jarduera indartu behar du. Bide horretan, Europako Itun Berdearen garapena oinarrizkoa izango da.Sustainable development is one of the objectives of the European integration process. The activity of the EU in recent years to achieve this objective is based on the energy transition. The analysis of European strategies and laws show the promotion of renewable energies and energy efficiency has become a tool that is being used for the protection of the environment, the fight against climate change and the promotion of the green economy. However, given the results, the achievement of the specific objectives seems to be slowing down, so the EU must strengthen its activity to complete the energy transition. The development of the European Green Deal is going to be fundamental.
Pelatihan Pemanfaatan Smartphone Android Sebagai Media Pembelajaran Bagi Orang Tua Kelurahan Sidokerto Kecamatan Bumi Ratu Nuban
Authors: Mariana Erni , Novita Novita , Wardany Kusuma , Sari Reni Permata 
Published: August 2021

This community service program has the following objectives, namely, 1) To introduce the use of an Android Smartphone with the Android application in the process of teaching and learning activities in class for parents/guardians of Sidokerto Village, Bumi Ratu Nuban District, Bumi Ratu Nuban District, 2) To increase the use of Android smartphones with the Android application for Parents / Guardians of Sidokerto Urban Village, Bumi Ratu Nuban District. The results of this dedication show that there is high enthusiasm from the participants during the delivery of the material and practices that are carried out. Apart from being active in providing responses and asking the participants, it also shows that the presentation given has been directly practised in the process of teaching and learning activities that they carry out. In this case, the participants feel they have had new experiences in the teaching and learning process, so the hope is that it can foster enthusiasm for learning and student creativity so that it can improve student achievement. Abstrak: Program pengabdian pada masyarakat ini memiliki tujuan yaitu, 1) Untuk mengenalkan pemanfaatan Smartphone android dengan aplikasi andorid dalam proses kegiatan belajar mengajar di kelas pada Orang Tua/Wali Murid Kelurahan Sidokerto Kecamatan Bumi Ratu Nuban, 2) Untuk peningkatan penggunaan pemanfaatan Smartphone android dengan aplikasi andorid bagi Orang Tua/Wali Murid Kelurahan Sidokerto Kecamatan Bumi Ratu Nuban. Hasil dari pengabdian ini menunjukkan bahwa adanya antusias yang tinggi dari peserta, selama penyampaian materi dan praktik yang dilakukan. Selain aktif memberikan tanggapan dan bertanya para peserta juga menunjukkan bahwa pemaparan yang disampaikan sudah dipraktikkan secara langsung dalam proses kegiatan belajar-mengajar yang mereka lakukan. Dalam hal ini peserta merasa mendapat pengalaman baru dalam proses belajar-mengajar, maka harapannya dapat menumbuhkan semangat belajar dan kreativitas siswa sehingga dapat meningkatkan prestasi siswa.
Edukasi dan pendampingan kader tentang manajemen diri lansia dengan komorbid diabetes melitus pada era pandemi COVID-19 di kelurahan Sidanegara Kabupaten Cilacap
Authors: Prasetyani Dewi , Rahayu Yuni Sapto Edhy 
Published: August 2021

Pasien Covid-19 dengan diabetes memiliki tingkat kematian yang tinggi, yaitu 7,8% dibandingkan pasien Covid-19 non diabetes. Puskesmas Cilacap Tengah 1 memiliki jumlah pasien diabetes terbanyak di Kabupaten Cilacap. Kegiatan pengabdian masyarakat ini dilakukan dalam dua tahap yaitu edukasi dan pendampingan kader posyandu lansia tentang manajemen diri lansia dengan DM. Sebagai langkah awal, penulis memberikan edukasi tentang manajemen diri DM kepada kader yang dihadiri oleh 19 orang kader perwakilan dari posyandu lansia yang ada di Kelurahan Sidanegara Kecamatan Cilacap Tengah. Hasil analisa data menunjukkan ada peningkatan pengetahuan kader tentang manajemen diri diabetes, dilihat dari nilai rata-rata pre-test 70,4 yang naik menjadi 76,5 pada nilai rata-rata post-test, dimana selisih nilai pre-test dan post-test adalah 6 poin. Kader juga mendapatkan buku manajemen diri DM sebagai buku pedoman. Sedangkan kegiatan pendampingan kader di posyandu lansia masih menunggu ijin dari pemerintah daerah Cilacap, karena selama pandemi Covid-19 kegiatan posyandu masih ditutup. Abstract: Covid-19 patients with diabetes have a high mortality rate, which is 7,8% compared to non-diabetic covid-19 patients. Central Cilacap Health Center 1 has the highest number of diabetes patients in the Cilacap Regency. This community service activity is carried out in 2 stages, namely education and mentoring of elderly posyandu kader regarding elderly self-management with DM. As a first step, the authors provide education about self-management of DM to kader which was attended by 19 kader from the elderly posyandu in Sidanegara Village, Cilacap Tengah District. The results of data analysis showed that there was an increase in kader’s knowledge about diabetes self-management, seen from the pre-test average score 70,4 which rose to 76,5 on the average post-test score, where the difference between the pre-test and post-test scores is 6 point. Kader also receives the diabetes self-management book as a guidebook. Meanwhile, kader assistance activities at the elderly posyandu are still waiting for permission from the Cilacap regional government, because during the covid-19 pandemic, posyandu activities were still closed.
Edukasi bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional kepada masyarakat Desa Air Salobar
Authors: Yunita Melda , Sukmawati S 
Published: August 2021

Abstrak: Masalah resistensi bakteri terhadap antibiotik tidak hanya terjadi di Indonesia tetapi juga secara global dan menjadi satu persoalan yang cukup rumit dan harus segera diatasi bersama-sama. Di masyarakat, kerap kali antibiotik dibeli tanpa penjelasan dan resep dokter. Masyarakat juga kerap membeli antibiotik dengan resep yang pernah diperoleh sebelumnya, bahkan langsung mengonsumsi antibiotik untuk penyakit ringan yang penanganannya tidak selalu membutuhkan antibiotik seperti demam, batuk dan pilek. Tujuan dari kegiatan pengabdian masyarakat ini ialah memberikan edukasi mengenai bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional kepada masyarakat desa Air salobar kecamatan Nusaniwe Ambon. Metode yang digunakan pada kegiatan ini adalah metode ceramah dengan memberikan materi mengenai bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional secara langsung. Hasil dari kegiatan pengabdian yang telah dilakukan menunjukkan bahwa masyarakat desa Air Salobar sangat antusias terhadap kegiatan yang dilaksanakan. Hal tersebut ditandai dengan antusiasnya, warga dalam menerima materi edukasi. Selain itu, warga yang hadir dalam kegiatan ini aktif memberikan respon timbal balik terhadap pemateri dengan cara memberikan pertanyaan-pertanyaan mengenai penyebab dan efek dari resistensi bakteri terhadap antibiotik. Abstract: Bacterial resistance issue does not only occur in Indonesia but also globally and becomes a quite complex problem and must be addressed together. Commonly, antibiotics are often purchased without a doctor’s explanation and prescription. People tend to buy antibiotics with prescriptions that have been obtained before, even directly consuming antibiotics for minor ailments whose treatment does not always require antibiotics such as fever, cough and cold. The purpose of this activity was to educate the community on the dangers of bacterial resistance due to irrational use of antibiotics to the community of Air Salobar Village, Nusaniwe district, Ambon. The method used was a lecture method by providing material on the dangers of bacterial resistance due to irrational use of antibiotics directly. The results showed that the residents of Air Salobar village are very enthusiastic about the community service activity carried out. This was indicated by the enthusiasm of the residents in receiving educational materials. Apart from that, the residents who attended this activity actively gave a reciprocal response to the presenters by asking questions about the causes and effects of bacterial resistance to antibiotics.
Characterization and anti-acute myeloid leukemia and anti-acute T cell leukemia properties of zinc nanoparticles synthesized by a green approach for bioremediation applications
Authors: Zou Min , Zhong Zhiqiang , Wen Chunju 
Published: August 2021

IntroductionEvery year, many people die due to cancer in all of the world. So, the preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects to treat cancer are a priority of both developing and developed countries. Recently, zinc nanoparticles have been used as modern chemotherapeutic drugs to treat several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In this study, zinc nanoparticles (ZnNPs) were synthesized in an aqueous medium using Fumaria officinalis leaf as stabilizing and reducing agents.Material and methodsThe green synthesized ZnNPs@Fumaria officinalis were characterized using different techniques including UV-visible and FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectrometry (EDS). The anticancer activity of ZnNPs@Fumaria officinalis was evaluated against acute myeloid leukemia and acute T cell leukemia. According to the XRD analysis, 20.44 nm was measured for the crystal size of ZnNPs@Fumaria officinalis.ResultsSEM images showed a uniform spherical morphology with an average size of 27.96 nm for ZnNPs@Fumaria officinalis. In the cellular and molecular part of the recent study, the cells treated with ZnNPs@Fumaria officinalis were assessed by MTT assay for 48 h regarding the cytotoxicity and anti-human acute leukemia properties on normal (HUVEC), acute myeloid leukemia (32D-FLT3-ITD and Human HL-60/vcr), and acute T cell leukemia (Jurkat, Clone E6-1 and J.RT3-T3.5) cell lines. In the antioxidant test, the IC50 values of ZnNPs@Fumaria officinalis and BHT against DPPH free radicals were 187 and 197 µg/ml, respectively. The IC50 values of ZnNPs@Fumaria officinalis were 227, 200, 250, and 336 µg/ml against 32D-FLT3-ITD, Human HL-60/vcr, Jurkat, Clone E6-1, and J.RT3-T3.5 cell lines, respectively. The viability of the malignant leukemia cell line decreased dose-dependently in the presence of ZnNPs@Fumaria officinalis.ConclusionsIt appears that the anti-human acute leukemia effect of ZnNPs@Fumaria officinalis is due to their antioxidant effects.