bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appraisal of Triglyceride-Related Markers as Early Predictors of Metabolic Outcomes in the PREVIEW Lifestyle Intervention: A Controlled Post-hoc Trial
Authors: Navas-Carretero Santiago , San-Cristobal Rodrigo , Siig Vestentoft Pia , Brand-Miller Jennie C. , Jalo Elli , Westerterp-Plantenga Margriet , Simpson Elizabeth J. , Handjieva-Darlenska Teodora , Stratton Gareth , Huttunen-Lenz Maija , Lam Tony , Muirhead Roslyn , Poppitt Sally , Pietiläinen Kirsi H. , Adam Tanja , Taylor Moira A. , Handjiev Svetoslav , McNarry Melitta A. , Hansen Sylvia , Brodie Shannon , Silvestre Marta P. , Macdonald Ian A. , Boyadjieva Nadka , Mackintosh Kelly A. , Schlicht Wolfgang , Liu Amy , Larsen Thomas M. , Fogelholm Mikael , Raben Anne , Martinez J. Alfredo 
Published: November 2021

Background: Individuals with pre-diabetes are commonly overweight and benefit from dietary and physical activity strategies aimed at decreasing body weight and hyperglycemia. Early insulin resistance can be estimated via the triglyceride glucose index {TyG = Ln [TG (mg/dl) × fasting plasma glucose (FPG) (mg/dl)/2]} and the hypertriglyceridemic-high waist phenotype (TyG-waist), based on TyG x waist circumference (WC) measurements. Both indices may be useful for implementing personalized metabolic management. In this secondary analysis of a randomized controlled trial (RCT), we aimed to determine whether the differences in baseline TyG values and TyG-waist phenotype predicted individual responses to type-2 diabetes (T2D) prevention programs.Methods: The present post-hoc analyses were conducted within the Prevention of Diabetes through Lifestyle intervention and population studies in Europe and around the world (PREVIEW) study completers (n = 899), a multi-center RCT conducted in eight countries (NCT01777893). The study aimed to reduce the incidence of T2D in a population with pre-diabetes during a 3-year randomized intervention with two sequential phases. The first phase was a 2-month weight loss intervention to achieve ≥8% weight loss. The second phase was a 34-month weight loss maintenance intervention with two diets providing different amounts of protein and different glycemic indices, and two physical activity programs with different exercise intensities in a 2 x 2 factorial design. On investigation days, we assessed anthropometrics, glucose/lipid metabolism markers, and diet and exercise questionnaires under standardized procedures.Results: Diabetes-related markers improved during all four lifestyle interventions. Higher baseline TyG index (p &lt; 0.001) was associated with greater reductions in body weight, fasting glucose, and triglyceride (TG), while a high TyG-waist phenotype predicted better TG responses, particularly in those randomized to physical activity (PA) of moderate intensity.Conclusions: Two novel indices of insulin resistance (TyG and TyG-waist) may allow for a more personalized approach to avoiding progression to T2D.Clinical Trial Registration:https://clinicaltrials.gov/ct2/show/NCT01777893 reference, identifier: NCT01777893.
Evaluation Expression of miR-146a and miR-155 in Non-Small-Cell Lung Cancer Patients
Authors: Dezfuli Neda K. , Alipoor Shamila D. , Dalil Roofchayee Neda , Seyfi Sharareh , Salimi Babak , Adcock Ian M. , Mortaz Esmaeil 
Published: November 2021

BackgroundNon−small-cell lung cancer (NSCLC) is the major type of lung cancer. MicroRNAs (miRNAs) are novel markers and targets in cancer therapy and can act as both tumor suppressors and oncogenes and affect immune function. The aim of this study was to investigate the expression of miR146a and miR155 in linked to blood immune cell phenotypes and serum cytokines in NSCLC patients.MethodsThirty-three NSCLC patients and 30 healthy subjects were enrolled in this study. The allele frequencies of potential DNA polymorphisms were studied using polymerase chain reaction (PCR)–restriction fragment length polymorphism (PCR-RFLP) analysis in peripheral blood samples. Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of miR-146a and miR-155 in peripheral blood mononuclear cells (PBMCs). Serum cytokine (IL-1β, IL-6, TNF-α, TGF-β, IL-4, IFN-γ) levels were determined by ELISA. The frequency of circulating CD3+CTLA-4+ and CD4+CD25+FOXP3+ (T regulatory cells/Treg) expression was measured by flow cytometry.ResultsmiR-146a was significantly downregulated in PBMC of NSCLC patients (P ≤ 0.001). Moreover, IL-6 and TGF-β levels were elevated in NSCLC patients (P ≤ 0.001, P ≤ 0.018, respectively). CD3+ CTLA-4+ and Treg cells frequencies were higher in patients than in control subjects (P ≤ 0.0001, P ≤ 0.0001, respectively). There was a positive correlation between miR-155 and IL-1β levels (r=0.567, p ≤ 0.001) and a negative correlation between miR-146a and TGF-β levels (r=-0.376, P ≤ 0.031) in NSCLC patients. No significant differences were found in the relative expression of miR-146a and miR-155, cytokine levels or immune cell numbers according to miR-146a and miR-155 (GG/GC/CC, TT/AT/AA) genotypes. However, there was a positive correlation between miR-146a and IL-1β levels (r=0.74, P ≤ 0.009) in GG subjects and a positive correlation between miR-146a expression and CD3+CTLA4+ cell frequency (r=0.79, P ≤ 0.01) in CC genotyped subjects. Conversely, a negative correlation between miR-146a expression and Treg cell frequency (r=−0.87, P ≤ 0.05) was observed with the GG genotype. A positive correlation between miR-155 and IL-1β expression (r=0.58, p ≤ 0.009) in the TT genotype and between miR-155 expression and CD3+CTLA-4 cell frequency (r=0.75, P ≤ 0.01) was observed in the AT genotype.ConclusionsThe current data suggest that the miR-146a expression in PBMC and serum TGF-β and IL-1β levels may act as blood markers in NSCLC patients. Further study is needed to elucidate the link between immune cells and serum miR146 at early disease stages.
Studies of Glyoxalase 1-Linked Multidrug Resistance Reveal Glycolysis-Derived Reactive Metabolite, Methylglyoxal, Is a Common Contributor in Cancer Chemotherapy Targeting the Spliceosome
Authors: Alhujaily Muhanad , Abbas Hafsa , Xue Mingzhan , de la Fuente Alberto , Rabbani Naila , Thornalley Paul J. 
Published: November 2021

BackgroundTumor glycolysis is a target for cancer chemotherapy. Methylglyoxal (MG) is a reactive metabolite formed mainly as a by-product in anaerobic glycolysis, metabolized by glyoxalase 1 (Glo1) of the glyoxalase system. We investigated the role of MG and Glo1 in cancer chemotherapy related in multidrug resistance (MDR).MethodsHuman Glo1 was overexpressed in HEK293 cells and the effect on anticancer drug potency, drug-induced increase in MG and mechanism of cytotoxicity characterized. Drug-induced increased MG and the mechanisms driving it were investigated and the proteomic response to MG-induced cytotoxicity explored by high mass resolution proteomics of cytoplasmic and other subcellular protein extracts. Glo1 expression data of 1,040 human tumor cell lines and 7,489 tumors were examined for functional correlates and impact of cancer patient survival.ResultsOverexpression of Glo1 decreased cytotoxicity of antitumor drugs, impairing antiproliferative activity of alkylating agents, topoisomerase inhibitors, antitubulins, and antimetabolites. Antitumor drugs increased MG to cytotoxic levels which contributed to the cytotoxic, antiproliferative mechanism of action, consistent with Glo1-mediated MDR. This was linked to off-target effects of drugs on glycolysis and was potentiated in hypoxia. MG activated the intrinsic pathway of apoptosis, with decrease of mitochondrial and spliceosomal proteins. Spliceosomal proteins were targets of MG modification. Spliceosomal gene expression correlated positively with Glo1 in human tumor cell lines and tumors. In clinical chemotherapy of breast cancer, increased expression of Glo1 was associated with decreased patient survival, with hazard ratio (HR) = 1.82 (logrank p &lt; 0.001, n = 683) where upper quartile survival of patients was decreased by 64% with high Glo1 expression.ConclusionsWe conclude that MG-mediated cytotoxicity contributes to the cancer chemotherapeutic response and targets the spliceosome. High expression of Glo1 contributes to multidrug resistance by shielding the spliceosome from MG modification and decreasing survival in the chemotherapy of breast cancer. Adjunct chemotherapy with Glo1 inhibitor may improve treatment outcomes.
Identification of miR-499a-5p as a Potential Novel Biomarker for Risk Stratification in Endometrial Cancer
Authors: Ravegnini Gloria , De Leo Antonio , Coada Camelia , Gorini Francesca , de Biase Dario , Ceccarelli Claudio , Dondi Giulia , Tesei Marco , De Crescenzo Eugenia , Santini Donatella , Corradini Angelo Gianluca , Tallini Giovanni , Hrelia Patrizia , De Iaco Pierandrea , Angelini Sabrina , Perrone Anna Myriam 
Published: October 2021

IntroductionThe Cancer Genome Atlas (TCGA) project identified four distinct prognostic groups in endometrial cancer (EC), among which two are correlated with an intermediate prognosis: the MisMatch Repair-deficient (MMRd) and the No Specific Molecular Profile (NSMP) groups. The two groups represent a heterogeneous subset of patients frequently harboring CTNNB1 alterations with distinctive clinicopathologic features. The study aimed to evaluate the miRNA expression in ECs to identify potential biomarkers of prognosis.MethodsWe analyzed miRNA expression in 72 ECs classified as MMRd or NSMP including 15 ECs with CTNNB1 mutations. In the discovery step, miRNA expression was evaluated in 30 cases through TaqMan miRNA arrays. Subsequently, four miRNAs were validated in the total cohort of ECs. The data were further tested in the TCGA cohort, and correlations with overall survival (OS) and progression-free interval (PFI) were evaluated.ResultsmiR-499a-3p and miR-499a-5p resulted to be overexpressed in CTNNB1 mutant EC patients at intermediate risk. Similarly, in the TCGA cohort, miR-499a-3p and miR-499a-5p were differentially expressed between CTNNB1 mutant and wild-type patients (p &lt; 0.0001). NSMP patients with low miR-499a-5p expression showed longer OS (p = 0.03, log-rank test). By combining miR-499a-3p or -5p expression levels with the CTNNB1 status, ECs with CTNNB1 mutation and lower miR-499a-5p expression showed better OS compared with the other subgroups (p = 0.03, log-rank test), among the NSMP patients. Moreover, in a multivariate analysis, combination of wild type CTNNB1 status and high miR-499a-5p expression was indipendently associated with high risk of death [HR (95%CI): 3.53 (1.1-10.5), p = 0.02].ConclusionOur results suggest that the combination of CTNNB1 status and miR-499a-5p allows a better stratification of NSMP patients and could promote a personalization of the treatment in intermediate-risk patients.
Reference Interval for Pulse Oxygen Saturation in Neonates at Different Altitudes: A Systematic Review
Authors: Wang Bo , Zhang Jia , Wu Ya-Zhen , Lu Zhi-Hui , Wang Na , Yu Zhang-Bin 
Published: November 2021

Introduction: The reference interval for pulse oxygen saturation (SpO2) in neonates born at high altitudes has not been defined to date. The purpose of this study was to systematically review published studies and determine the reference interval of SpO2 in neonates at different altitudes.Methods: Databases of PubMed, Embase, Cochrane Library, Clinicaltrials.Gov, Chinese National Knowledge Infrastructure Database, Wanfang Database, Chinese Science Technology Journals Database, and Chinese Clinical Trial Registry were searched for studies reporting SpO2 in healthy neonates at different altitudes. Retrieval time was from inception of the database to August 16, 2021. The Agency for Healthcare Research and Quality checklist was used to evaluate the quality of studies. Python v3.8 was used to analyze the data. This systematic review was drafted in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement.Results: Seven cross-sectional studies, published between 1991 and 2020, were identified. They were from US, Mexico, Israel, Ecuador, and China. Three studies were rated as high quality and four as moderate quality. The mean SpO2 (with standard deviation or standard error) of neonates born in 40 different altitudes (ranging from 25 meters to 3,100 meters) were obtained. The prediction equation for calculation of the lower limit of the reference interval was established, and the reference intervals for SpO2 at different altitudes were determined.Conclusions: In healthy neonates, the lower limit of the reference interval of SpO2 decreases with increase in altitude. High-quality prospective studies are need to confirm our findings.
Formación en prácticas comunitarias en la facultad de psicología de la UNR. Problemáticas y estrategias didácticas desde los enfoques socio-críticos en educación
Authors: Aita Gaspar Manuel 
Published: November 2021

Desde el Área II: “Prácticas Comunitarias desde la perspectiva psi para la Promoción de la Salud Mental”, perteneciente a la asignatura Práctica Profesional Supervisada “A” (Facultad de Psicología de la Universidad Nacional de Rosario), el presente artículo tiene como intención principal reflexionar críticamente en torno a los procesos de formación académica vinculados al campo comunitario desde las perspectivas psi en el nivel de pre-grado.
Es decir, se pretende compartir el estado de situación en que se encuentra este campo en nuestra Facultad y, a partir de ello, pensar posibles estrategias didácticas particulares, posicionados en lo teórico e ideológico desde los enfoques socio-críticos en educación
Desde nuestra experiencia docente, la propuesta emergente está orientada al análisis crítico de aquello que nos acontece dentro de las aulas durante el proceso de enseñanza y aprendizaje para lo comunitario, para habilitarnos a la posibilidad de pensar una didáctica especial y específica, una forma estructurada, sistematizada y planificada, según criterios propios e inherentes al campo de reflexión y acción en las comunidades.
Biotecnología y genética: el mapa de la acreditación. la primera fase del proceso de acreditación ante CONEAU
Authors: A. Rossi Leandro 
Published: November 2021

El presente trabajo propone realizar un relevamiento de la acreditación de carreras de Biotecnología y Genética a fin de esbozar un mapa general sobre la primera fase. Para ello se realizó la revisión y análisis de las resoluciones emitidas por la CONEAU sobre cada una de las carreras evaluadas en el marco de la normativa general vigente (Ley de Educación Superior – N° 24.521) y la normativa específica establecida por el Ministerio de Educación y la CONEAU.
A los fines del análisis, hemos planteado nuestro trabajo en dos partes sustantivas: por un lado, el marco normativo general y específico que regula los procesos de acreditación de carreras declaradas de interés público y, por otra parte, su aplicación en el caso de las carreras de Biotecnología y Genética a partir de tres ejes de estudio.
Entre los resultados podemos visibilizar cual es la cobertura geográfica de la oferta de carreras, la fortaleza en la función investigación, la alta formación docente y las posibilidades de mejora en extensión.
Pengaruh Media Poster Terhadap Kepatuhan Pasien Diabetes Melitus Tipe 2 Di Apotek Sahabat Denpasar Bali
Authors: Luh Putu Indah Arviani  , Oktianti Dian 
Published: October 2021

Diabetes Mellitus is a disease characterized by hyperglycemia and disturbances of carbohydrate, fat and protein metabolism associated with absolute or relative deficiency of insulin action and secretion.The success of Diabetes Mellitus treatment is very affected by the patient compliance to maintain their health. The purpose of this research to know the effect of providing drug information with poster to the level of Type 2 Diabetes Mellitus patients taking medication compliance at Sahabat Pharmacy.The research method used in this research was Cross sectional with quasi-experimental by pretest-postest, with total sample of 28 people, by using MMAS-8 questionnaires (Morisky Medication Compliance Scale), analyzed by using not in pairs t-test. The percentage of adherence before being given drug information with poster media was 92.85% adherence, moderate adherence 7.15%. Patient compliance before being given drug information with poster media was low adherence 21.42%, moderate adherence 71.42%, and high adherence 7.15%. Giving drug information with poster media has an effect on increasing medication adherence in type 2 diabetes mellitus patients with a significance value of 0.000.
ABSTRAK
Diabetes Melitus adalah penyakit yang ditandai dengan terjadinya hiperglikemia dan gangguan metabolisme karbohidrat, lemak dan protein yang dihubungkan dengan kekurangan secara absolut atau relatif dari kerja dan atau sekresi insulin. Keberhasilan pada suatu pengobatan Diabetes Mellitus sangat dipengaruhi oleh kepatuhan penderita untuk menjaga kesehatannya. Tujuan penelitian ini untuk mengetahui adanya pengaruh pemberian informasi obat dengan media poster terhadap tingkat kepatuhan minum obat pasien Diabetes Mellitus Tipe 2 di Apotek Sahabat. Metode penelitian yang digunakan pada penelitian ini adalah Cross sectional bersifat eksperimen semu dengan pretest-postest, menggunakan jumlah sampel sebanyak 26 orang, dengan kuesioner MMAS-8 (Morisky Medication Compliance Scale), dianalisis menggunakan uji t-test berpasangan. Persentase kepatuhan pasien sebelum diberi informasi obat dengan media poster adalah kepatuhan rendah 92,85%, kepatuhan sedang 7,15%. Kepatuhan pasien sesudah diberi informasi obat dengan media poster adalah kepatuhan rendah 21,42%, kepatuhan sedang 71,42%, dan kepatuhan tinggi 7,15%. Pemberian informasi obat dengan media poster berpengaruh terhadap peningkatan kepatuhan minum obat pada pasien diabetes mellitus tipe 2 dengan nilai signifikansi 0,000.
Pemberdayaan Ibu-Ibu Posyandu Sentolo Lor Melalui Edukasi Bahaya Pembalut dan Ecoliteracy: Upaya Preventif Permasalahan Kesehatan Reproduksi & Lingkungan
Authors: Basri Anindita Imam , Utaminingsih Siti Nur , Tisya Valsa Ayunda 
Published: November 2021

AbstrakPembalut merupakan kebutuhan primer bagi setiap perempuan yang telah mengalami masa pubertas. Namun, banyak kalangan wanita yang tidak terlalu memperdulikan personal hygiene pada saat penggunaan pembalut sekali pakai, sehingga berisiko terhadap kesehatan organ reproduksi. Berdasarkan hasil observasi di Dusun Sentolo Lor, Kecamatan Sentolo, Kabupaten Kulon Progo, Provinsi Daerah Istimewa Yogyakarta. masih banyak remaja dan ibu-ibu yang utamanya belum mengetahui dan memahami bahaya pemakaian pembalut sekali pakai. Kegiatan ini bertujuan untuk menumbuhkan kesadaran bahaya pembalut sekali pakai, cara menjaga personal hygiene, dan mengajak mitra untuk beralih ke pembalut ramah lingkungan. Metode pelaksanaan yang digunakan yaitu penyuluhan (ceramah), diskusi, tanya jawab, serta evaluasi program. Hasil evaluasi program menunjukkan adanya peningkatan signifikan dari setiap indikator setelah edukasi dilaksanakan. Dengan demikian, kegiatan pengabdian ini telah berhasil meningkatkan kualitas sumber daya manusia di Dusun Sentolo Lor. Kata Kunci: Pemberdayaan, Posyandu, Pembalut, Personal Hygiene. AbstractSanitary napkins are a primary need for every woman who has experienced puberty. However, many women do not really care about personal hygiene when using disposable sanitary napkins, so it poses a risk to the health of the reproductive organs. Based on the results of observations in Sentolo Lor Hamlet, Sentolo District, Kulon Progo Regency, Yogyakarta Special Region Province. there are still many teenagers and mothers who mainly do not know and understand the dangers of using disposable sanitary napkins. This activity aims to raise awareness of the dangers of single-use sanitary napkins, how to maintain personal hygiene, and invite partners to switch to environmentally friendly sanitary napkins. The implementation method used is counseling (lecture), discussion, question and answer, and program evaluation. The results of the program evaluation showed a significant increase in each indicator after the education was implemented. Thus, this service activity has succeeded in improving the quality of human resources in Dusun Sentolo Lor.Key Word: Empowerment, Posyandu, Sanitary Napkins, Personal Hygiene.
TAHAP PENGETAHUAN MASYARAKAT KELANTAN TERHADAP KEGANASAN RUMAH TANGGA
Authors: Ayob Nuruaslizawati , Abdul Rauf Siti Hajar , Samsudin Nur Masliza 
Published: November 2021

Abstract: Domestic violence is a serious social problem and can affect the health and well-being of its victims. Domestic violence statistic showed that there were an increasing number of domestic violence cases from the year 2014 to 2019. The main objective of this study is to examine the knowledge level of the Kelantanese people on the issue of domestic violence. This study used a quantitative research design through the distribution of questionnaires to obtain raw data. The selection was made randomly with a sample of 200 respondents. The data collected was analyzed using Statistical Package for Social Science (SPSS) version 17.0. The results of the study suggest that the level of knowledge of the people of Kelantan on the issue of domestic violence is at a high level with the mean value = 3.82 and standard deviation = 0.99. The findings of the study found that the majority of Kelantanese have a high knowledge on the issue of domestic violence. However, the number of domestic violence cases is relatively high. Thus, social support and encouragement from various parties such as friends, family and the authorities are necessary to reduce cases of domestic violence in the community.
Keywords: Domestic violence, Physical abuse, Emotional abuse, Economic abuse, Sexual abuse

Abstrak: Keganasan rumah tangga merupakan masalah sosial yang serius dan mampu memberi kesan kepada kesihatan serta kesejahteraan hidup seseorang. Statistik keganasan rumah tangga menunjukkan terdapat peningkatan dalam laporan mengenai kes keganasan rumah tangga yang berlaku pada tahun 2014 hingga 2019. Tujuan utama kajian ini adalah untuk melihat sejauh mana tahap pengetahuan masyarakat Kelantan terhadap isu keganasan rumah tangga. Kajian ini menggunakan reka bentuk kuantitatif melalui edaran borang soal selidik bagi mendapatkan data mentah. Pemilihan sampel dibuat secara rawak mudah ke atas 200 orang responden. Kajian dianalisis menggunakan perisian Statistical Package for Science Social (SPSS) versi 17.0. Hasil kajian mendapati tahap pengetahuan rakyat Kelantan terhadap isu keganasan rumah tangga berada pada tahap yang tinggi iaitu nilai min= 3.82 dan sisihan piawai= 0.99. Dapatan kajian mendapati bahawa majoriti rakyat Kelantan mempunyai pengetahuan yang tinggi terhadap isu keganasan rumah tangga. Namun begitu, jumlah kes keganasan rumah tangga yang berlaku di Negeri Kelantan adalah agak tinggi. Justeru, sokongan dan dorongan daripada pelbagai pihak seperti rakan, keluarga dan pihak berwajib adalah perlu bagi mengurangkan masalah kes keganasan rumah tangga dalam masyarakat.
Kata kunci: Keganasan rumah tangga, Keganasan fizikal, Keganasan emosi, Keganasan ekonomi, Keganasan seksual