bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 envelope-protein corruption of homeostatic signaling mechanisms in mammalian cells
Authors: Schulze Tobias , Hartel Andreas , Höler Sebastian , Hemming Clara , Lehn Robert , Tandl Dominique , Greiner Timo , Bertl Adam , Shepard Kenneth , Moroni Anna , Thiel Gerhard , Rauh Oliver 
Published: June 2021

AbstractDuring a SARS-CoV2 infection, host cells produce large amounts of the viral envelope protein (Ep-CoV2). Ep-CoV2 is partially inserted into the membrane of nascent viral particles and into cellular membranes. To mimic the pathophysiological impact of the cellular protein fraction, Ep-CoV2 was overexpressed in mammalian cells and effects on key signaling parameters were monitored. By tagging with green fluorescent protein (GFP), we found that Ep-CoV2 protein is mostly present in the endoplasmic reticulum with additional trace amounts in the plasma membrane. We observed that wild-type Ep-CoV2 and, to a lesser extent, its mutants (N15A, V25F) corrupted some of the most important homeostatic mechanisms in cells. The same was observed with isolated transmembrane domains of the protein. The Ep-CoV2-evoked elevation of intracellular Ca2+ and pH as well as the induced membrane depolarization produced by the presence of the protein interfere with major signal transduction cascades in host cells. These functions of Ep-CoV2, which likely contribute to the pathogenesis of the viral protein, result from the ion-channel activity of the viral protein. Two independent assays, a functional reconstitution of Ep-CoV2 protein in artificial membranes and a rescue of K+-deficient yeast mutants, confirm that Ep-CoV2 generates a cation-conducting channel with a low unitary conductance and a complex ion selectivity. The data presented here suggest that specific channel function inhibitors of Ep-CoV2 can provide cell protection and virostatic effects.
A molecular census of midbrain dopaminergic neurons in Parkinson’s disease
Authors: Kamath Tushar , Abdulraouf Abdulraouf , Burris SJ , Gazestani Vahid , Nadaf Naeem , Vanderburg Charles , Macosko Evan Z 
Published: June 2021

AbstractMidbrain dopamine (DA) neurons in the substantia nigra pars compacta (SNpc) project widely throughout the central nervous system, playing critical roles in voluntary movements, reward processing, and working memory. Many of these neurons are highly sensitive to neurodegeneration in Parkinson’s Disease (PD), and their loss correlates strongly with the pathognomonic symptoms. To characterize these populations molecularly, we developed a protocol to enrich and transcriptionally profile DA neuron nuclei from postmortem human SNpc of both PD patients and matched controls. We identified a total of ten distinct populations, including one that was primate-specific. A single subtype, marked by the gene AGTR1, was highly susceptible to degeneration, and was enriched for expression of genes associated with PD in genetic studies, suggesting many risk loci act within this subtype to influence its neurodegeneration. The AGTR1 subtype also showed the strongest upregulation of TP53 and its downstream targets, nominating a potential pathway of degeneration in vivo. The transcriptional characterization of differentially disease-vulnerable DA neurons in the SNpc will inform the development of laboratory models, enable the nomination of novel disease biomarkers, and guide further studies of pathogenic disease mechanisms.
Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells
Authors: Liu Yang , Zhao Ning , Kanemaki Masato T. , Yamamoto Yotaro , Sadamura Yoshifusa , Ito Yuma , Tokunaga Makio , Stasevich Timothy J. , Kimura Hiroshi 
Published: June 2021

ABSTRACTIn eukaryotic nuclei, chromatin loops mediated through cohesin are critical structures that regulate gene expression and DNA replication. Here we demonstrate a new method to visualize endogenous genomic loci using synthetic zinc-finger proteins harboring repeat epitope tags (ZF probes) for signal amplification via binding of tag-specific intracellular antibodies, or frankenbodies, fused with fluorescent proteins. We achieve this in two steps. First, we develop an anti-FLAG frankenbody that can bind FLAG-tagged proteins in diverse live-cell environments. The anti-FLAG frankenbody complements the anti-HA frankenbody, enabling two-color signal amplification from FLAG and HA-tagged proteins. Second, we develop a pair of cell-permeable ZF probes that specifically bind two endogenous chromatin loci predicted to be involved in chromatin looping. By coupling our anti-FLAG and anti-HA frankenbodies with FLAG- and HA-tagged ZF probes, we simultaneously visualize the dynamics of the two loci in single living cells. This reveals close association between the two loci in the majority of cells, but the loci markedly separate upon the triggered degradation of the cohesin subunit RAD21. Our ability to image two endogenous genomic loci simultaneously in single living cells provides a proof-of-principle that ZF probes coupled with frankenbodies are useful new tools for exploring genome dynamics in multiple colors.
Molecular structures of the pentaphenylcyclopentadienyl iron complexes [(C5Ph5)Fe(CO)2
R] (R = Me, Ph, iPr and Bu)
Authors: Sünkel Karlheinz , Klein-Hessling Christian 
Published: June 2021

The PdII-catalysed reaction of [(C5Ph5)Fe(CO)2Br] with Grignard compounds RMgX or butyl lithium gave the iron alkyl/aryl complexes [(C5Ph5)Fe(CO)2
R] (R = Me, Ph, iPr and Bu) in 59–73% yield, namely, dicarbonylmethyl(η5-pentaphenylcyclopentadienyl)iron, [Fe(CH3)(C35H25)(CO)2], dicarbonyl(η5-pentaphenylcyclopentadienyl)phenyliron, [Fe(C6H5)(C35H25)(CO)2], dicarbonyl(isopropyl)(η5-pentaphenylcyclopentadienyl)iron, [Fe(C3H7)(C35H25)(CO)2], and butyldicarbonyl(η5-pentaphenylcyclopentadienyl)iron, [Fe(C4H9)(C35H25)(CO)2]. The crystal structure determinations showed the usual `paddle-wheel' orientation of the phenyl rings, with an average canting angle of ca 50°. The bond parameters are mainly dictated by the steric requirements of the alkyl/aryl groups and only the phenyl complex shows electronic effects.
The level matters: building capabilities for innovation and enterprise performance through customer involvement
Authors: Anning-Dorson Thomas 
Published: June 2021

PurposeThis paper aims to examine the quadratic relationship between customer involvement capability and performance, and innovation of small- and medium-scale service firms. The study answers the critical question of how SMEs make the most benefit from their involvement capability.Design/methodology/approachData from small and medium enterprises (SMEs) operating in the service sector of an emerging economy in sub-Saharan Africa were used. A two-stage data analysis with a quadratic estimation was used to assess the hypothesized relationships.FindingsThe study found that the influence of involvement capability over SME performance differs in terms of financial and non-financial. And that, while increasing customer involvement will improve non-financial performance such as customer satisfaction and service quality, it is only at the intermediate level that financial performance is optimized.Originality/valueThe relationships (involvement, and performance and innovation) are not linear, and that at some levels, the relationship with financial or non-financial performance is more positive than other levels. The findings also suggest that involvement capability influences both process and product innovations most at intermediate levels than at low and high levels.
Validity and reliability of the Italian version of the Teachers' Sense of Efficacy Scale
Authors: Pintus Andrea , Bertolini Chiara , Scipione Lucia , Antonietti Maja 
Published: June 2021

PurposeThis study investigates the construct validity and reliability of the Italian version of the Teachers' Self-Efficacy Scale (TSES) developed by Tschannen-Moran and Hoy (2001) and contributes to existing research aimed at exploring the elements and variables that influence teachers' self-efficacy levels.Design/methodology/approachThe instrument, translated and adapted in Italian by Cardarello et al. (2017), was tested on a sample of 349 in-service second grade teachers with similar demographic profiles (gender, age, seniority). The study analyzes the data following the exploratory factor analysis.FindingsThe results of this research confirm the relevance of the three-factor model, but opens the possibility of a different organizational structure of the items within the scale. In fact, the results confirmed both the original TSES three-factor structure (student engagement, classroom management, instructional strategies) and a new four-factor structure. The new four-factor structure recognizes two new factors of an instructional nature, “Inclusive instructional strategies, learner-centered” and “Traditional instructional strategies, teacher-centered.” The first one recalls beliefs about capabilities to use strategies and assessments focused on students' needs (inclusive perspective); the second one refers to the dimension of the disciplinary master.Originality/valueAccording to the idea that teachers' self-efficacy is a multidimensional construct, the findings of this study invite to reflect upon the meanings of scale application in different cultural contexts and open a discussion about instructional strategies and the experience of teaching in Italy.
Performance assessment of the operations strategy of credit unions
Authors: Martins Poliana , Pinheiro de Lima Edson , Bortoluzzi Sandro César , Gouvea da Costa Sergio E. 
Published: June 2021

PurposeIn this study, the performance assessment of credit unions is developed, seeking to demonstrate how the content of their operations strategy is present in the structuring of unique models of organisational performance assessment. The main objective is to compare sources of knowledge through the content analysis of the service operations strategy as proposed by the literature and validated by specialists.Design/methodology/approachThis research is a qualitative study that uses the knowledge of specialists to frame a performance assessment structure applied to credit unions. The research strategy is based on multicriteria analysis, and the selected techniques are based on the MCDA-C procedure, that is, the processes of structuring, evaluating and recommending improvements.FindingsThe research results provided: a data set external to the context that serves as theoretical support for the comparisons made; the structuring of 4 unique organisational performance assessment models carried out through the MCDA-C procedure; conceptual interpretation using the lens of the operations strategy in the cognitive maps of the assessment models, which identifies the operations strategy carried out; and comparison of the result models that show their uniqueness.Originality/valueThe comparison between the origins of knowledge is highlighted, and the demonstration of the content was proposed by the literature and by specialists to evaluate the performance in operations. This was then compared with the content constructed with credit union managers in structuring models based on the singularities of the respective contexts. The theory was compared to practice, and it was demonstrated how the content of the operations regarding the performance could be used in decision contexts.
Design of logarithmic crowned roller for tapered roller bearings based on the elastohydrodynamic lubrication model
Authors: Duan Hongyu , Yu Qingtao , Wang Zhijian 
Published: June 2021

Purpose
The purpose of this paper is to study the film-forming capacity of logarithmic crowned roller for tapered roller bearing (TRB) and to design a tapered roller profile based on an elastohydrodynamic lubrication model.

Design/methodology/approach
A coupled model, incorporating a quasi-static model of TRBs and an elastohydrodynamic lubrication model was developed to investigate the load distribution of TRB and to evaluate the lubrication state of tapered roller/raceway contact.

Findings
The model is verified with published literature results. Parametric analysis is conducted to investigate the effect of crown drop on azimuthal load distribution of the roller, film thickness and pressure distribution in the contact area. The result shows that crown drop has little influence on the azimuthal load distribution; also, the film thickness and the pressure distribution are asymmetric. When the tapered roller is designed and manufactured, the crown drop of the small end should be larger than that in the large end.

Originality/value
Precise roller profile design is conducive to improve the fatigue life of TRBs. Currently, most crown design methods neglect the influence of lubrication, which can lead to a non-suitable roller profile. Therefore, the present work is undertaken to optimize roller profiles based on lubrication theory.
“Felt responsibility”: a mediator for balancing NGOs’ upward and downward accountability
Authors: Chu Vien , Luke Belinda 
Published: June 2021

Purpose
This study aims to investigate how non-government organisation (NGO) managers balance accountability to donors and beneficiaries and the role of felt responsibility in this process.

Design/methodology/approach
Using concepts of accountability theory, practices of microenterprise development NGOs are examined in two countries – Bangladesh and Indonesia – through interviews with managers of 20 NGOs and analysis of NGOs’ publicly available data.

Findings
Findings show a shift in emphasis from a vertical view (upward to donors and downward to beneficiaries) to a horizontal view of NGO accountability. Under this view, a selective approach to donors whose mission and approaches to poverty alleviation aligned with those of the NGOs played an essential role in supporting NGOs’ internal accountability. Further, felt a responsibility to beneficiaries is identified as an important mediator balancing both upward and downward accountability. While accountability to donors and beneficiaries was interrelated, accountability to donors was considered a short-term objective and accountability to beneficiaries was considered a long-term and overriding objective.

Originality/value
Findings contribute a further understanding of the role of felt responsibility to beneficiaries as a mediator for balancing upward and downward accountability based on the perspectives of NGO managers. Reframing accountability through a horizontal view helps to balance multiple directions of NGO accountability: to self, donors and beneficiaries.
Promoting fearlessness of change through social intelligence: mediating role of collective efficacy and moderating role of management commitment to change
Authors: Goswami Meghna 
Published: June 2021

PurposeThis study aims to investigate the influence of social intelligence and collective efficacy on the fearlessness of change. Furthermore, this study investigates the mediation effect of collective efficacy and moderating role of management commitment to change in the relationship between social intelligence and fearlessness of change.Design/methodology/approachThe analysis is based on data collected from 296 members of information technology and Banking Financial Services Industry organisations using a survey questionnaire. Hypotheses have been tested using structural equation modelling.FindingsThe findings show that social intelligence and collective efficacy positively influence fearlessness of change. Social intelligence also impacts collective efficacy positively. Further, collective efficacy acts as a mediator and management commitment to change acts as a moderator in the relationship between social intelligence and fearlessness of change.Research limitations/implicationsThis study highlights the relevance of social intelligence in fostering a fearless attitude towards change for easy transition from the current organisational state to a new or desired state.Practical implicationsOrganisational leaders must strive to develop a climate of fearlessness in organisations undergoing change so that the employees acquire this attitude of fearlessness and face the hurdles that come with change with a positive mind set.Originality/valueThis study is amongst the few such studies that examined the relationship amongst variables of this study.
Knowing your audience: understanding urban superintendents' process of framing equitable change
Authors: Coviello James , DeMatthews David E. 
Published: June 2021

PurposeThe purpose of this study is to understand how superintendents leading large, high-profile and politically complex urban districts make sense of their district–community context and advocate for issues of equity.Design/methodology/approachThis qualitative multi-case study took place over the 2017–18 school year and involved superintendents leading large urban districts in the United States, with data derived from semi-structured interviews, observations of school board and other public meetings and document collection.FindingsThis article describes how superintendents' sensemaking around equity was situated within the context of interactions with district board members and other stakeholder groups within their communities and influenced by their sense of professional vulnerability of public advocacy. Leaders often felt the need to attenuate their personal sense of equity and act strategically when framing related policies or practices. This study highlights examples by which superintendents were forced to confront instances when community support and prioritization of equity issues did not match their own and subsequently struggled to make sense of how to frame issues that were not in alignment.Originality/valueDespite their positional authority, relatively little attention has been paid to the experience of school district superintendents in fostering equity. This study provides practical examples of superintendents making sense of complex leadership scenarios and taking strategic action to promote equity in authentic circumstances and has important implications for research and practice.
Sustainable prosperity and enterprises for Maori communities in Aotearoa New Zealand: a review of the literature
Authors: Neha Tia , Macfarlane Angus , Macfarlane Sonja , Clarke Te Hurinui , Derby Melissa , Torepe Toni , Duckworth Fiona , Gibson Marie , Whelan Roisin , Fletcher Jo 
Published: June 2021

Purpose
The research in the field of Indigenous peoples and the espousal of their cultural values in the work environment is recognised as being important as a means of overcoming workplace inequities. The purpose of this paper is to examine research about Maori, the Indigenous people of Aotearoa New Zealand that may inform future enterprises for the long-term prosperity of marginalised Indigenous peoples.

Design/methodology/approach
This study reviews the literature on unique cultural dynamics of whanau Maori (New Zealand Maori family/community) study practices and the importance of work/home/life balance. Furthermore, it considers strengths-based community enterprises that can lead to sustainable prosperity for Maori.

Findings
The review yielded three theoretical principles that explain mana (sociocultural and psychological well-being), which can be generalised across multiple contexts, with the workplace being one of these contexts. These principles of mana create a contextual match with whanau external realities; an experiential match of a mana empowerment framework that transfers to the study context and an interpersonal understanding of being understood and empowered within the study context.

Research limitations/implications
The literature review has been limited to research from 2005 onwards and to research that investigates Maori, the Indigenous peoples of Aotearoa New Zealand. Although the review of the literature has these limitations, the review may be of interest to other studies of Indigenous peoples worldwide.

Practical implications
The key factors are interwoven, and their importance is considered in relation to the development of positive and supportive environments, which link to job retention, satisfaction and productivity in the workplace for Maori. This, in turn, can have beneficial knock-on effects for not only the New Zealand economy but also more importantly for enhancing sustainable livelihoods for upcoming generations.

Social implications
Tied together, these factors are paramount for cultural, social and ecological benefits for nga rangatahi (young Maori adults) and the wider community in the workplace.

Originality/value
The literature review’s value and originality derive from a dearth of recent research on supporting nga rangatahi (young Maori adults) for sustainable prosperity.
Applying machine learning approach to predict students’ performance in higher educational institutions
Authors: Yakubu Mohammed Nasiru , Abubakar A. Mohammed 
Published: June 2021

Purpose
Academic success and failure are relevant lifelines for economic success in the knowledge-based economy. The purpose of this paper is to predict the propensity of students’ academic performance using early detection indicators (i.e. age, gender, high school exam scores, region, CGPA) to allow for timely and efficient remediation.

Design/methodology/approach
A machine learning approach was used to develop a model based on secondary data obtained from students’ information system in a Nigerian university.

Findings
Results revealed that age is not a predictor for academic success (high CGPA); female students are 1.2 times more likely to have high CGPA compared to their male counterparts; students with high JAMB scores are more likely to achieve academic success, high CGPA and vice versa; students from affluent and developed regions are more likely to achieve academic success, high CGPA and vice versa; and students in Years 3 and 4 are more likely to achieve academic success, high CGPA.

Originality/value
This predictive model serves as a classifier and useful strategy to mitigate failure, promote success and better manage resources in tertiary institutions.