bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between cancer-related fatigue and diabetes from pre-chemotherapy to 6-months post-chemotherapy
Authors: Kleckner Amber , Kleckner Ian R. , Culakova Eva , Shayne Michelle , Belcher Elizabeth K. , Gudina Abdi , Williams AnnaLynn M. , Onitilo Adedayo A. , Hopkins Judith O. , Gross Howard , Mustian Karen M. , Peppone Luke J. , Janelsins Michelle C. 
Published: February 2021

Abstract
Purpose To quantify the impact of diabetes on the trajectory of cancer-related fatigue (CRF) from pre-chemotherapy to 6 months post-chemotherapy for patients with breast cancer compared to non-cancer controls.Methods This was a secondary analysis from a nationwide prospective longitudinal study of female patients with breast cancer undergoing chemotherapy and age-matched women without cancer (controls). CRF was measured using the Multidimensional Fatigue Symptom Inventory (MFSI) pre-, post-, and 6-months post-chemotherapy in patients; controls were assessed at equivalent time points. Diabetes status was obtained at baseline. Repeated measures mixed models estimated the association between CRF and diabetes controlling for cancer (y/n), body mass index, exercise and smoking habits, baseline anxiety and depressive symptoms, menopausal status, marital status, race, and education.Results A total of 439 patients and 235 controls (age: 52.8±10.5 years) had available data on diabetes status. Diabetes was twice as prevalent among patients as controls (11.6% vs. 6.8%). Patients had worse fatigue than controls throughout treatment (p<0.001). Diabetes was associated with worse CRF with a clinically meaningful difference of 4.7±1.7 points on the fatigue measure in all participants (p=0.009) and patients alone (p=0.030). For MFSI subdomains, diabetes was associated with worse general (p=0.002), physical (p=0.005), and mental fatigue (p=0.025) but not worse emotional fatigue or vigor (p>0.14) among patients. Conclusions Diabetes was twice as prevalent in women with breast cancer compared to controls, and diabetes was associated with more severe CRF in patients before and after chemotherapy and at 6 months post-chemotherapy. Interventions that address diabetes management may also help address CRF during chemotherapy treatment
The Role of SIRT5&nbsp;and p53&nbsp;Proteins in the Sensitivity of Colon Cancer Cells to Chemotherapeutic Agent 5-Fluorouracil&nbsp;
Authors: Ekremoglu Ozlem , Koc Asli 
Published: February 2021

Abstract
In the treatment of colorectal cancer, it is important to develop drug combinations that will increase the effectiveness of chemotherapy and to determine the molecular targets of the drugs. Therefore, combined therapies that can increase the sensitivity of 5-Fluourouracil (5-FU), a chemotherapeutic agent used in the treatment of colon cancer, and also the molecular pathways involved in this process are important in the treatment of the disease. Here we examined the SIRT5 (Resveratrol and Suramin) and p53 (Nutlin3a) modulators alone or in combination with 5-FU on the proliferation of colon cancer cells and effect of 5-FU on the SIRT5 and FOXO3a expressions whether p53 dependent or independent manner. p53 protein expression is activated by nutlin3a in HCT-116 p53 +/+ cells and HCT-116 p53 -/- cells were used as counterpart cells that did not express p53. According to our MTT assay results, we showed that these modulators have significant effects on the sensitivity of both cells to 5-FU. In p53+/+ cells, Resveratrol (RSV) and Nutlin3a and in p53 -/- cells, Suramin was found to be more effective. These differences were evaluated together with the effect of 5-FU on the SIRT5-FOXO3a-Bim axis in p53 +/+ and p53 -/- cells. SIRT5 is known to deactivate FOXO3a which plays a role in the induction of apoptosis via Bim. Our western blot experiment results showed that while Suramin decreased SIRT5 and RSV decreased FOXO3a protein expressions significantly in HCT-116 p53 -/- cells, 5-FU decreased significantly SIRT5 and FOXO3a protein expressions p53 independently. These results may help the discovery of new markers in colon cancer treatment.
Flavonoid 3'-hydroxylase of Camellia Nitidissima Chi. Can Promote the Synthesis of Polyphenols Better Than Flavonoids
Authors: Jiang Lina , Fan Zhengqi , Tong Ran , Yin Hengfu , Li Jiyuan , Zhou Xingwen 
Published: February 2021

Abstract
Camellia nitidissima Chi is an ornamental plant of the genus Camellia L. Its flowers contain a lot of flavonoids and polyphenols. Flavonoid 3'-hydroxylase (F3'H) plays an important role in the synthesis of flavonoids, polyphenols and anthocyanins. We cloned CnF3'H from the petal of C. nitidissima (GenBank code: HQ290518.1). The full length of CnF3'H was 1859 bp, with an open reading frame of 1577 bp, and encoded 518 amino acid. A phylogenetic tree analysis showed that CnF3'H was closely related to Camellia sinensis L. and C. sinensis cultivar Zhonghuang. CnF3'H was expressed in flowers, leaves, fruits, sepals, petals and stamens of C. nitidissima, and during the flowering process the expression level in flower decreased initially and then increased. CnF3'H expression was significantly positive correlated with polyphenol contents in C. nitidissima. A CnF3'H-EGFP expression vector was constructed to do the subcellular localization, we found that CnF3'H was obviously localized in the nuclear envelope and cytomembrane. In transgenic tobacco flowers, the total polyphenol content and various polyphenol constituents were significantly increased with high CnF3'H expression level, while total flavonoid contents and some flavonol constituents were increased slightly. These findings suggest that CnF3'H can promote the synthesis of polyphenols better than flavonoids.
Urinary Volatiles and Chemical Characterisation for the Non-invasive Detection of Prostate and Bladder Cancers&nbsp;
Authors: Tyagi Heena , Daulton Emma , Bannaga Ayman S , Arasaradnam Ramesh P , Covington James A. 
Published: February 2021

Abstract

Bladder cancer (BLC) and Prostate cancer (PC) are some of the most common cancers in the world, both having low five-year survival rates. In both BLC and PC, the diagnosis is often confirmed with an invasive technique that carries a risk to the patient. Consequently, a non-invasive diagnostic approach in these cancers would be medically desirable. The use of volatile organic compounds (VOCs) for disease diagnosis, including cancer, is a promising research area that could support the diagnosis process. In this study, we investigated the urinary VOC profiles in BLC, PC patients and non-cancerous controls by using gas chromatography–ion mobility spectrometry (GC–IMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) to analyse patient samples. GC-IMS separated BLC from PC (AUC: 0.97 (0.93-1.00)), BLC Vs Non-cancerous (AUC: 0.95 (0.90-0.99).) and PC Vs Non-cancerous (AUC: 0.89 (0.83-0.94)) whereas GC-TOF-MS differentiated BLC from PC (AUC: 0.84 (0.73-0.93) BLC Vs Non-cancerous (AUC: 0.81 (0.70-0.90)) and PC vs Non-cancerous (AUC: 0.94 (0.90-0.97)). According to our study, a total of 34 biomarkers were found using GC-TOF-MS data, of which 13 VOCs were associated with BLC, 7 were associated with PC and 14 VOCs were found in the comparison of BLC and PC.
Oral Treponema denticola infection induces Aβ1-40 and Aβ1-42 accumulation in the hippocampus of C57BL/6 mice
Authors: Su Xinyi , Tang Zhiqun , Liu Yuqiu , He Wanzhi , Jiang Jiapei , Zhang Yifan , Wu Hongkun 
Published: February 2021

Abstract
Accumulation of amyloid-β (Aβ) in the brain is a central component of pathology in Alzheimer’s disease. A growing number of evidences demonstrate close associations between periodontal pathogens including Porphyromonas gingivalis (P. gingivalis) and Treponema denticola (T. denticola) and AD. However, the effect and mechanisms of T. denticola on accumulation of Aβ remain to be unclear. In this study, we demonstrated that T. denticola was able to enter brain and act directly on nerve cells resulting in intra and extracellular Aβ1−40 and Aβ1−42 accumulation in the hippocampus of C57BL/6 mice by selectively activating both β-secretase and γ-secretase. Furthermore, both KMI1303, an inhibitor of β- secretase, as well as DAPT, an inhibitor of γ- secretase were found to be able to inhibit the effect of T. denticola on Aβ accumulation in N2a neuronal cells. Overall, it is concluded that T. denticola increases the expression of Aβ1−42 and Aβ1−40 by its regulation on beta-site amyloid precursor protein cleaving enzyme-1 and Presenilin 1.
Novel semi-synthetic Cu (II)-cardamonin complex exerts potent anticancer activity against triple negative breast and pancreatic cancer cells via inhibition of the Akt signaling pathway
Authors: Hossan Md Shahadat , Break Mohammed Khaled Bin , Bradshaw Tracey D. , Collins Hilary M. , Wiart Christophe , Khoo Teng-Jin 
Published: February 2021

Abstract

Triple negative breast cancer (TNBC) and pancreatic cancer are two of the most aggressive types of cancer that lack effective treatments. We have previously reported the semi-synthesis of a novel Cu (II)-cardamonin complex (19) that demonstrated potent anti-tumour activity. In this study, we further investigated the bioactivity and mode of action of 19 against MDA-MB-468 and PANC-1 cancer cells in an attempt to discover an effective anticancer agent for TNBC and pancreatic cancer, respectively. Results revealed that 19 abolished formation of MDA-MB-468 and PANC-1 colonies, exerted growth inhibitory activity and inhibited the migration of cancer cells. Further mechanistic studies showed that it induced DNA damage resulting in G2/M-phase arrest and microtubule network disruption. Moreover, there was an increase in ROS production which may have contributed to the observed induction of apoptosis, corroborated by activation of caspase-3/7, cleavage of PARP and downregulation of Mcl-1. Complex 19 also decreased the expression levels of p-Akt and p-4EBP1 in both cell lines, which indicates that the compound exerted its activity, at least in part, via inhibition of the Akt signaling pathway. Furthermore, it decreased the expression of c-Myc in PANC-1 cells only which suggests that it may exert its bioactivity via multiple mechanisms of action pertinent to tumourigenesis. These results demonstrate the potential of 19 as a promising therapeutic agent for TNBC and pancreatic cancer.
Comparison of clinical outcomes achieved with oblique lateral interbody fusion with or without supplementary instrumentation in patients with single-level lumbar degenerative disease
Authors: Lang Zhao , Sun Yuqing , Yuan Qiang , Wu Jingye , Fan Mingxing , Li Jianing , Tian Wei 
Published: February 2021

Abstract
Background

Oblique lateral interbody fusion (OLIF) is applied often to treat degenerative disc disease in the lumbar spine. Stand-alone OLIF prevents morbidities associated with supplemental fixation and is less expensive. However, it remains controversial whether stand-alone OLIF is sufficient to avoid subsidence for single-level diseases. Additionally, bilateral pedicle screw (BPS) and bilateral transfacet screw (BTS) fixation are well-established posterior fixation methods that can offer improved biomechanical stability. But the comparison of clinical outcomes of OLIF with and without supplementary instrumentation is lack.
Methods

We retrospectively examined 20 patients who underwent single-level stand-alone OLIF for symptomatic lumbar degenerative disease at L1–L5 (SA group). Groups of patients treated with OLIF plus BPS (n = 20, BPS group) or BTS (n = 20, BTS group) were matched for age, sex, diagnosis, operative level, body mass index, and bone mineral density. The disk height index (DHI), segmental lordotic (SL) angle, and lumbar lordotic (LL) angle were measured preoperatively and at 3 days and 6 months postoperatively. Clinical outcomes were evaluated.
Results

Significant disc height loss was observed in all groups, but was greater in the SA and BTS groups than in the BPS group at the 6-month follow-up. The SL and LL angles were not affected in any group. The operative time was significantly less in the SA group, and the estimated blood loss was significantly higher in the BPS group. At 6 months post-surgery, improvements in clinical outcomes were evident in all groups, but the VAS (back pain), JOA, and ODI scores were worse in the SA group than in the other groups.
Conclusions

Stand-alone OLIF was associated with greater subsidence and poorer clinical outcomes compared with OLIF plus supplementary instrumentation. The addition of BTS did not decrease the degree of subsidence, but provided clinical outcomes comparable to those achieved with BPS.
Postoperative Hypoalbuminemia: Prevalence, Risk Factors, Association with Postoperative Pneumonia in Brain Tumors Patients Undergoing Craniotomy
Authors: Zhao Da-wei , Zhang Xu-yang , Wei Kai-yan , Jiang Yi-bin , Liu Dan , Zhang Shui-xian , Feng Hua , Hu Rong 
Published: February 2021

Abstract
Purpose

Hypoalbuminemia is associatied with to poor outcome in patients undergoing surgery intervention. The main aim for this study was to investigate the incidence and the risk factors of postoperative hypoalbuminemia and assessed the impact of postoperative hypoalbuminemia on postoperative complications in patients undergoing brain tumor surgery.
Methods

This retrospective study included 372 consecutive patients who underwent craniotomy for brain tumors from January 2017 to December 2019. The demographic data, pre- and post-operative laboratory tests and postoperative complications were collected. The patients were divided into two groups based on the postoperative serum albumin levels; hypoalbuminemia group (< 35 g/L) and non-hypoalbuminemia group (≥ 35 g/L). Univariate and multivariate logistic regression analyses were used to determine risk factors of postoperative hypoalbuminemia and complications.
Results

Of the total 372 patients underwent craniotomy due to brain tumor, 333 patients (89.5%) developed hypoalbuminemia after surgery. Hypoalbuminemia was associated with operation time (OR 1.011, P < 0.001), preoperative albumin (OR 0.864, P = 0.015) and peroperative globulin (OR 1.192, P = 0.004). The incidence of postoperative pneumonia in patients with hypoalbuminemia (41.1%) significantly higher than that in patients without hypoalbuminemia (23.1%) (P = 0.029). The independent predictors of postoperative pneumonia were age (OR 1.053, P < 0.001), operation time (OR 1.003, P = 0.013) and lower postoperative albumin (OR 0.946, P = 0.018).
Conclusions

Postoperative hypoalbuminemia has a higher incidence with the increase of operation time, and is associated with postoperative pneumonia in patients underwent craniotomy due brain tumor.
Effect of Antiepileptic Drugs in Glioma Patients on Self-reported Depression, Anxiety, and Cognitive Complaints&nbsp;
Authors: Meer Pim B. van der , Koekkoek Johan A.F. , Bent Martin J. van den , Dirven Linda , Taphoorn Martin J.B. 
Published: February 2021

Abstract
IntroductionAEDs have been associated with depression, anxiety, and cognitive impairment, all frequent complications of glioma and its subsequent treatment, with considerable morbidity and an adverse effect on health-related quality of life. This study aimed to determine the independent association between AED use and self-reported depression, anxiety, and subjective cognitive impairment in glioma patients. Methods In this multicenter cross-sectional study depression and anxiety were measured with the HADS and subjective cognitive impairment was measured with the MOS-CFS. Univariable logistic regression analyses were performed on all possible confounding predictor variables, and if p-value<0.1, possible confounders were included in the multivariable analyses to identify whether use of AEDs was independently related to depression, anxiety, and/or subjective cognitive impairment.ResultsA total of 272 patients were included. Prevalence of depression differed significantly between patients not using (10%) and using AEDs (21%, unadjusted Odds Ratio [uOR]=2.29 [95%CI=1.05-4.97], p=0.037), but after correction for confounders the statistical significant difference was no longer apparent (adjusted Odds Ratio [aOR]=1.94 [95%CI=0.83-4.50], p=0.125). Prevalences of anxiety (aOR=1.17 [95%CI=0.59-2.29], p=0.659) and subjective cognitive impairment (aOR=0.83 [95%CI=0.34-2.04], p=0.684) did not differ significantly before or after adjustment of confounders between patients not using (19% and 16%, respectively) and using AEDs (26% and 21%, respectively).ConclusionsOur results indicate AED use was not independently associated with concurrent depression, anxiety, or subjective cognitive impairment in glioma patients. Alternative factors seem to have a greater contribution to the risk of having these neuropsychiatric symptoms in glioma patients.
WITHDRAWN: High SLC7A11 Expression Is Correlated With Poor Prognosis and Associated With Ferroptosis in Ovarian Cancer&nbsp;
Authors: Deng Mengqi , Zhang Yanqin , Chang Xiangyu , Wu Di , Xu Chunyu , Liu Xinyu , Zhang Yubo , Miao Jinwei 
Published: February 2021

Abstract
The current treatments of ovarian cancer (OC) do not yield satisfactory outcomes. Hence, it is necessary to find new treatment targets for OC. In this study, a comprehensive bioinformatic analysis was conducted to identify differentially expressed genes (DEGs) between OC and control tissues. Five datasets were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were screened by comparing gene expression between OC and control tissues. Module analysis of DEGs was performed on the STRING database and GEPIA. Kaplan Meier plotter and GEPIA database analysis the overall survival. Finally, SLC7A11 was found to be is the hubgene. And we confirm that the protein expression of SLC7A11 was increased in OC tissues. Analysis of a variety of tumor gene databases showed that SLC7A11 gene regulated the processes of OC. The low mutation rate of the gene (which were of amplified type) and high mRNA expression were associated with poor prognosis of OC patients.Using erastin-treated ovarian cancer (OC) cell lines, we examined the relationship between ferroptosis and OC. Results showed that OC tissues contained higher malondialdehyde (MDA) levels than normal tissues. Unlike normal ovarian epithelial cells which are not sensitive to erastin, the OC cell line, ES-2 is very sensitive to erastin. Here, we found that ferrostatin-1 treatment increased levels of reactive oxygen species (ROS), malondialdehyde, and SLC7A11 protein expression. These results provide an important theoretical basis for further studies into the role of SLC7A11, the effective biomarker and potential drug target, in the occurrence and development of OC.
Aberrant Hydroxymethylation In Promoter Cpg Regions of Genes Related to the Cell Cycle and Apoptosis Characterizes Advanced Chronic Myeloid Leukemia Disease, Poor Imatinib Respondents And Poor Survival.
Authors: Guru Sameer Ahmad , Sumi Mamta P , Mir Rashid , Beg Mirza Masroor Ali , Koner Bidhan Chandra , Saxena Alpana 
Published: February 2021

Abstract
Background: There is strong evidence that disease progression, drug response and overall clinical outcome of CML disease are not only decided by BCR/ABL1 oncoprotein but depend on accumulation of additional genetic and epigenetic aberrations. DNA hydroxymethylation is implicated in the development of variety of diseases. DNA hydroxymethylation in gene promoters plays an important role in disease progression, drug response and clinical outcome of various diseases. Therefore in this study, we aimed to explore the role of aberrant hydroxymethylation in promoter regions of different tumor suppressor genes in relation to CML disease progression, response to imatinib therapy and clinical outcome. We recruited 150 CML patients at different clinical stages of the disease. Patients were followed up for 48 months and haematological/molecular responses were analysed. Haematological response was analysed by peripheral blood smear. BCR/ABL1 specific TaqMan probe based qRT-PCR was used for assessing the molecular response of CML patients on imatinib therapy. Promoter hydroxymethylation of the genes was characterized using MS-PCR.Results: We observed that promoter hydroxymethylation of cell cycle regulating and apoptosis related genes characterize advanced CML disease and poor imatinib respondents. Although, cytokine signalling (SOCS1) gene was hypermethylated in advanced stages of CML and accumulated in patients with poor imatinib response, but the differences were not statistically significant. Moreover, we found hypermethylation of cell cycle regulating genes (p14ARF, RASSF1 and p16INK4A) and cytokine signalling gene (SOCS1) significantly associated with poor overall survival of CML patients on imatinib therapy. The results of this study are in agreement of the role of aberrant DNA methylation of different tumor suppressor genes as potential biomarkers of CML disease progression, poor imatinib response and overall clinical outcome. Conclusion: In this study, we report that promoter hydroxymethylation of cell cycle and apoptosis related genes is a characteristic feature of CML disease progression, defines poor imatinib respondents and poor overall survival of CML patients to imatinib therapy.
Electronic Media Use and Sleep in Children and Adolescents: A Systematic Review
Authors: Lund Lisbeth , Sølvhøj Ida Nielsen , Danielsen Dina , Andersen Susan 
Published: February 2021

Abstract
Background In recent years, there has been an increasing interest in whether the use of electronic media affects children and young adolescents' sleep. We performed a systematic review of the association between electronic media use and sleep outcomes among 0-15-year-olds.Methods Searches were carried out in four databases (CINAHL, Web of Science, EMBASE, and Medline). Additional studies were identified by hand-searching reference lists of retrieved systematic reviews and meta-analyses. Inclusion criteria were age range from 0 to 15.9 years, in English, and intervention studies, cohort studies, and cross-sectional studies from western countries. Qualitative studies were excluded due to a low number of identified studies. Exclusion criteria were study populations with psychiatric diagnoses, obesity, or any sleep disorder. Results The search identified 10,719 unique studies, of which 108 fulfilled the inclusion and exclusion criteria and were assessed for methodological quality. In total, 49 studies were included in the review. The study designs were randomized controlled trials (n=3), quasi-experimental studies (n=two), prospective cohort studies (n=15), and cross-sectional studies (n=29). Evidence for an association between electronic media use and sleep duration was identified; particularly excessive electronic media use and bedtime use. The evidence was stronger for 6-15-years-olds than 0-5-year-olds. The relationship between electronic media use and other sleep outcomes was more inconclusive. Conclusions Overall, electronic media use was generally associated with shorter sleep duration in children and adolescents aged 0-15. Studies with stronger research design and of higher quality are needed to draw solid conclusions about electronic media’s impact on sleep outcomes and gain better understands of the mechanisms behind the associations. Public awareness and interventions could be promoted about the potential negative impact on children's sleep duration of electronic media devices that are used excessively and close to bedtime.