bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of leuprolide acetate-loaded calcium phosphate silicate cement to bone regeneration under osteoporotic conditions
Authors: Meng Lisha , Li Yajin , Wang Yu , Zhang Jingshu , Zhang Yubiao , Chen Yadong , Gong Tianxing 
Published: June 2021

Abstract
Osteoporosis is detrimental to the health of skeletal structure and significantly increases the risks of bone fracture. Moreover, bone regeneration is adversely impaired by increased osteoclastic activities as a result of osteoporosis. In this study, we developed a novel formulation of injectable bone cement based on calcium phosphate silicate cement (CPSC) and leuprolide acetate (LA). Several combinations of LA-CPSC bone cement were characterized and, it is found that LA could increase the setting time and compressive strength of CPSC in a concentration-dependent manner. Moreover, the in vitro results revealed that LA-CPSC was biocompatible and able to encourage the osteoblast proliferation via the mTOR signalling pathway. Furthermore, the LA-CPSC was implanted in the osteoporotic rats to evaluate its effectiveness to repair bone fractures under the osteoporotic conditions. The biomarker study and micro-CT analyses indicated that LA-CPSC could effectively reduce the osteoclast activities and promote the bone regeneration. In conclusion, our study demonstrated that LA-CPSC injectable bone cement should be a viable solution to repair bone fractures under the osteoporotic conditions.
Composition, density and spatial distribution of zooplankton on 1st transition season (March to May) in Inner Ambon Bay Waters, Indonesia
Authors: Pello F S , Latumeten J , Tetelepta J M S 
Published: June 2021

Abstract
Inner Ambon Bay is part of Ambon Bay that covered an area about 11.03 km2. It is semi-closed and become a small pelagic fish fishing ground, especially anchovy. The anchovy is a zooplankton predator, hence, the abundance of anchovy is dependent on the abundance of zooplankton. The research aims to analyze the composition, density, and spatial distribution of the zooplankton during the 1st transition season (March to May). Data of zooplankton composition were attained from sampling by using plankton net at ten observation stations, meanwhile, data of densities were obtained using a scientific hydroacoustic system on six parallel transect lines and one cross-parallel transect line. The study shows that the zooplankton community is dominated by Copepod and meroplankton. The highest density was found in March, while the lowest density was in April. Vertical distribution of zooplankton shows that the highest density was found near the surface (0-4 m) and decrease to a deeper water column. On the horizontal distribution, lower densities (0 – 400 ind./m2) occupy a wider area, they are evenly distributed in western, middle, and eastern, in the contrary, higher densities (3,000-4,000 ind./m2) occupy smaller space viz. in the southwest and east of Inner Ambon Bay.
Species diversity, density, phosphate concentration and the utilization of algae as a food material
Authors: Litaay C , Arfah H , Rugebregt M J , Opier R D A 
Published: June 2021

Abstract
The influence of nature and human activities greatly affects the distribution and diversity of algae. This study was conducted to determine the species diversity, density, phosphate concentration, and algae utilization as a food material found on the island of Ambon. The sampling location was on Ambon’s island in three locations, i.e., Tantui, Hative Besar, and Allang. For 100 m, line transects were laid perpendicular to the shore at every station. For every of the transect line, four 50 cm×50 cm quadrat was placed randomly. There was 18 genus of seaweed. Of the 22 species, 11 species from Rhodophyta (50.0 %), 5 species from Phaeophyta (22.73%), and 6 were from the class of Chlorophyta (27.3%). The highest value density is village Hative Besar that is 1219.9 gr.m−2 is from the Chlorophyta group (Enteromorpha prolifera), Tantui is 986 gr/m2 from the Rhodophyta group (Gracilaria), and Allang that is 756.18 gr.m−2 from the Rhodophyta group (Acantophora). The maximum concentration in Allang is 0.19 mg.L−1, concentration in Hative Besar is 0.18 mg.L−1, while the minimum phosphate concentration is 0.15 mg.L−1 at the Tantui location. The types of algae that can be used as food sources include Gracilaria sp., Caulerpa sp., Sargassum sp, Gelidium sp., and Ulva sp.
Biology aspect and parameter population the scalloped hammerhead (Sphyrna lewini Griffith & Smith, 1834) caught from coastal fisheries in the eastern Indian Ocean
Authors: Chodrijah U , Faizah R 
Published: June 2021

Abstract
The scalloped hammerhead, Sphyrna lewini, have a circumglobally distribution in tropical and warm-temperate waters. Generally, in Indian ocean this species is a bycatch of the tuna longline, however, in Tanjung Luar, this species is a target species. The life history from this species is still limited. The study aimed to assess the biology aspect and growth of the scalloped hammerhead in the period February 2013 to December 2016. The result showed that 2449 individuals were caught with size between 43-395 cm TL (average 198,03 cm) for females and males ranged between 47-340 cm TL (average 161.61 cmTL). The sex ratio that was dominated by female sharks was 2.69: 1. The clasper length and total length relationship were positive with a linear regression equation of CL = 0.0902*TL-4.4402 (R2=0.6837). It showed that the length of the claspers increases proportionally with the length of the body. Application of age at length data for produced von Bertalanffy growth model parameter estimates of female L∞ = 397 cm TL, K = 0.27 year−1, for male S. lewini was L∞ = 381 cm TL, K = 0.17/ year. The total mortality rate (Z) the scalloped hammerhead was 1.15/ years, natural mortality (M) is 037/ years and the fishing mortality (F) was 0.78/ years with exploitation rate (E) = 0.68
Management policy of small-scale tuna fisheries based on island cluster in Maluku
Authors: Tauda I , Hiariey J , Lopulalan Y , Bawole D 
Published: June 2021

Abstract
Tuna is one of essential economic fish commodities in Maluku which mostly caught by handline and trolling line small scale fishery with fishing vessel capacity under 5 GT. The fishing vessel numbers and fish production of this fishery are declining recently, and the catching was predominantly by small size. The highest yellowfin tuna production was found in Island Cluster 7 amounted to 9,107 tones or 31% of total yellowfin tuna production in Maluku by 2019. This study was aimed to analyze the condition of yellowfin tuna in Maluku and to propose a management policy for small-scale tuna fishery base on Island Cluster. Result shows that the production between 2013-2019 was stagnant with a declining tendency. The implementation of island cluster approach in small-scale tuna fishery management will increase the efficiency, productivity, and competitiveness on it, especially at the potential island clusters 7, 5, and 4. Of all-island clusters found, island cluster 7 having the highest performance indicator for small-scale tuna fishery.
Sea urchin identification in Kodingareng Lompo waters, Spermonde Archipelago
Authors: Moka W , Inaku D F , Rais M. , Moka W 
Published: June 2021

AbstractSea urchin, belongs to Phylum Echinoderm, Class Echinoidea. It is categorized as benthic marine invertebrates, and could be found in several ecosystem from shallow water to abyssal depth. Despite the high potential, there is no detail information of this organism in Indonesia, especially in Spermonde Archipelago, South Sulawesi Region. This study aim to identify the sea urchin species found in Kodingareng Lompo Island, Spermonde Archipelago. Kodingareng Lompo is one of the islands belong to Sangkarang Regency, Makassar city. Sampling was done during July 2020. Samples were collected randomly in seagrass bed and transition area. The morphological identification was conducted at the main island. The morphological traits of sea urchin samples collections were observed, noted, and compared with some literatures for identification. There three kind of sea urchin found in this waters:Diadema setosum, Mespilia globulusandTripneustes gratilla.
Ranching of sea cucumber in Indonesia : A study case of Holothuria atra
Authors: Hartati R , Zainuri M , Ambariyanto A , Widianingsih W , Mustagpirin  , Panji F 
Published: June 2021

Abstract
Sea ranching is one of the promising sustainable ways to restore depleted stocks and increase production of sea cucumber. Before, only high price species were exploited but now the demand is extended to many other low-value species, such as Teripang Hitam, H. atra. The present work were aimed to observed the growth and survival of H. atra ranching using pen system. Sea pens system size of 5meter x 5meter x 1.8meter was set up in Teluk Awur Waters, Jepara, Indonesia. They were designed as mesocosms that mimic sea ranching location. Wild young H. atra (average weight of 100 g) from Panjang Island, located adjacent to Teluk Awur waters were stocked at 3 individu.m−2, reared for five months without food addition. Sea cucumber was weighed and counted monthly to determine their growth and survival rate. The results of present experiment revealed that sea cucumber increased their weight with the time of rearing, the average weight gain and survival rates were 212 g and 83% respectively. It showed that their adaptation to the new environment was went well. The sea cucumber got intake of food that naturally grew in the sea pens or transported by the sea current from surrounding area. This result showed promising application on sea ranching for marine natural conservation.
Production function model of African catfish hatchery business in Joho village, Wates, Kediri
Authors: Aisyah D , Supriyadi S , Sari M , Alfarizi W , Asshovani C 
Published: June 2021

Abstract
The use of production inputs on African catfish hatchery business can cause production risks also can reduce production risks. This study aims to analyze the effect of production inputs cause on production risks faced by African catfish hatchery cultivators in Joho Village, Wates, Kediri. This study took 30 respondents conducted by snowball sampling and the data analysis method used Cobb-Douglas model. Data processing tools used Microsoft Excel 2016 and R-Studio 4.0.1 statistical software. The results showed production inputs of artificial feed, broodstock, and drugs significantly affect productivity. Increased of broodstock significantly decreases production risk, and increased of medicines significantly increase production risk. The policy recommendations of this study are limiting the use of production inputs that could pose production risk, and good fish farming implementation methode based on the policy of Directorate General of Aquaculture (DJPB).
CD4 (Cluster Differentiation-4) as an immune response to the portal of entry mechanism of VNN (Viral Nervous Necrosis) infection in grouper
Authors: Yanuhar U , Anitasari S , Kusriani  , Junirahma N S , Caesar N R 
Published: June 2021

AbstractViral Nervous Necrosis (VNN) is a Nodaviridae virus that attacks groupers, especially in the larval and seed stages. VNN attacks the brain organs, rapidly attacks the grouper fish receptors and then spreads to the brain via blood circulation. The mechanism of viral infection can occur because of the bond between the VNN adhesive and its receptor molecules in the grouper organ. The aim of this study was to compare the CD4 (Cluster Differentiation-4) values of VNN-infected groupers and healthy fish using immunohistochemical (IHC) analysis. The method used in this study was experimental exploratory by comparing and analyzing the response of CD4 cells in healthy groupers with groupers infected with VNN through IHC observations. The results of IHC test showed that the immunogenic epitope of 32.5 kDa VNN protein had the ability to induce tissue receptors of Humpback grouper (Cromileptes altivelis) by forming immune cells qualitatively. There are CD4 cells that are immune to specific tissue organs, i.e. eyes of the groupers, which are labeled with anti-CD4 secondary antibodies. It seems that immunogenic protein VNN with a molecular weight of 32.5 kDa has the ability to stimulate the expression of Humpback grouper immune cells such as CD4 in tissue organs, especially the eye organs qualitatively.
Sustainable tourism development based on local participation: Case study on Dalegan District for the East Java tourism industry
Authors: Rahmawati D , Soedarso  , Suryani A , Wibowo B M , Muklason A , Endarko  
Published: June 2021

Abstract
Coastal areas and small islands has considerable management potential, however several studies have shown that these conditions do not make the management of coastal areas in Indonesia optimal. The purpose of this research is to try to answer important issues regarding how sustainable tourism development affects people’s lives and livelihoods. This research presents debates in sustainable tourism development by paying attention to the concept of sustainable development and investigating the main problems of developing sustainable tourism development. The methodology used in this research is qualitative by using focus group discussions (FGD) with 50 people in the village of Dalegan to obtain data from important informants. The results of the research show that the lack of attention to the environmental sector in the village makes coastal tourism objects less attractive to tourists. The development of local communities must also go hand in hand with sustainable tourism development, the relationship between government and coastal managers in Dalegan also needs strengthening. The perception of visiting experiences reinforced by the presence of local communities is essential to increase the number of tourists to create a conducive economic climate. We suggest that the socio-economy should coexist with environmental sustainability and a sustainable tourism development framework must be created to address heterogeneity and complexity in a regional scope.
Dilution and Aspect Ratio Properties on Performance of Laser Deposited Ti-Al-Si-Cu/Ti-6Al-4V Composite Coatings
Authors: Fatoba O.S. , Naidoo LC. , Akinlabi S.A. , Akinlabi E.T. , Mwema F.M. , Ikumapayi O.M. 
Published: June 2021

Abstract
In spite of the fact that Laser additive manufacturing (AM) processes have a similar material additive manufacturing theory, each AM procedure has its particular features in terms of operational materials and applicable situations. In recent years, additive manufacturing techniques have been acquainted as a promising technique to generate metallic parts since they can annihilate the familiar problems associated with conventional techniques. The results of the investigation showed that as the transverse speed increases, the material that is distributed to the substrate per unit length decreases and therefore that there is an inverse relationship with clad width, melt pool depth and clad height. Hence as the scanning speed increases the width if the height is smaller and the depth of the pool will be smaller too. The results indicate that the specimens manufactured at 900 W, had a decrease in dilution by 3.83%, 1.29% for Ti-Al-11Si-5Cu and Ti-Al-12Si-2Cu respectively when the scanning speed is increased. However, for Ti-Al-13Si-6Cu, there is an increase in the dilution by 1.06%, which can be attributed to the increase of the deposit area below the substrate than above. The results evidently show that increasing the laser scanning speed, increases the aspect ratio of the specimens for laser processing condition 900 W. This relationship is observed in attribution to the clad height being reduced as the scan speed is increased. Moreover, the varying scanning speed influences the clad height more significantly than the clad width; hence, the significant increase in the aspect ratio.
Sustainability of Infrastructural Projects in Nigeria: A Critical Review
Authors: Ongbali S. O. , Akinyemi A. A. , Afolalu S. A , Leramo R. O. , Ombugadu M. G. 
Published: June 2021

Abstract
Government at all levels in Nigeria implement diverse projects intended for economic development and benefit of the public such as roads, electricity and water supplies, ports, transportation, telecommunications, schools, health facilities, housing, and railways among other projects. However, some of these projects fail to reach completion and commissioning and sometimes abandoned on account of numerous factors. This paper seeks to appraise the current efforts in the topic area to identify and provide a holistic document on the key factors to blame for public project failure and abandonments in Nigeria which are fragmentally documented in the literature. The finding will chart the road map for intervention by stakeholders to alleviate the problem for sustainable economic development and improve the quality of life of the citizens. It appeared from the descriptive statistical analysis of the 44-variables identified in the literature that the underlying factors causing Government project failure include lack of financial capacity, use of incompetent contractors based on political consideration, corruption, poor design and implementation, poor project planning and scheduling, inaccurate cost estimate and payment delay amid others. We recommend that the Government address the identified key variables to enhance infrastructure development to boost the economy and improve the quality of life. Additionally, further research is required to use advanced analytical tools to examine the 44-variables identified which were previously neglected to provide reliable information on the key factors causing the problem for appropriate intercession by Government and stakeholders.
Effects of intercalated water on the lubricity of sliding layers under load: a theoretical investigation on MoS2
Authors: Stella Martina , Lorenz Christian D , Clelia Righi Maria 
Published: June 2021

Abstract
Two-dimensional (2D) materials, such as graphene and molybdenum disulfide (MoS2) have recently become some of the most studied nano-materials due to their wide array of technological and industrial applications. Among these, they display great potential as solid lubricants. Friction properties of 2D-materials, however, are very sensitive to environmental conditions, e.g. humidity. In MoS2, for instance, humidity can hamper its tribologic performances. Past experiments and recent ab-initio molecular dynamics simulations have highlighted that, at ordinary temperatures, a possible reason for lower lubricity is the physical interaction of water with the layers. It is, therefore, crucial to better understand the microscopic mechanisms underlying this behaviour, in order to optimise the lubrication performance of these materials. In this paper we used density functional theory simulations and classical molecular dynamics simulations to provide a multi-scale description of how external load affects the energetic, structural and dynamic properties of intercalated water between MoS2 layers. As a result of combining these two different approaches, we provide an atomistic description of the role of intercalated water in modifying the frictional behaviour of physically interacting layers, e.g. MoS2. The identified interlocking mechanism, which is enhanced under load, is relevant for understanding the frictional effects observed for water confined in slit nanopores, and for nanofluidics applications.
A Problem for the Ideal Worlds Account of Desire
Authors: Blumberg Kyle 
Published: June 2021

AbstractThe Ideal Worlds Account of Desire says that S wants p just in case all of S’s most highly preferred doxastic possibilities make p true. The account predicts that a desire report ⌜S wants p⌝ should be true so long as there is some doxastic p-possibility that is most preferred (by S). But we present a novel argument showing that this prediction is incorrect. More positively, we take our examples to support alternative analyses of desire, and close by briefly considering what our cases suggest about the logic of desire.
EcTracker: Tracking and elucidating ectopic expression leveraging large-scale scRNA-seq studies
Authors: Gautam Vishakha , Mittal Aayushi , Kalra Siddhant , Mohanty Sanjay Kumar , Gupta Krishan , Rani Komal , Naidu Srivatsava , Mishra Tripti , Sengupta Debarka , Ahuja Gaurav 
Published: June 2021

Abstract
Dramatic genomic alterations, either inducible or in a pathological state, dismantle the core regulatory networks, leading to the activation of normally silent genes. Despite possessing immense therapeutic potential, accurate detection of these transcripts is an ever-challenging task, as it requires prior knowledge of the physiological gene expression levels. Here, we introduce EcTracker, an R-/Shiny-based single-cell data analysis web server that bestows a plethora of functionalities that collectively enable the quantitative and qualitative assessments of bona fide cell types or tissue-specific transcripts and, conversely, the ectopically expressed genes in the single-cell ribonucleic acid sequencing datasets. Moreover, it also allows regulon analysis to identify the key transcriptional factors regulating the user-selected gene signatures. To demonstrate the EcTracker functionality, we reanalyzed the CRISPR interference (CRISPRi) dataset of the human embryonic stem cells differentiated into endoderm lineage and identified the prominent enrichment of a specific gene signature in the SMAD2 knockout cells whose identity was ambiguous in the original study. The key distinguishing features of EcTracker lie within its processing speed, availability of multiple add-on modules, interactive graphical user interface and comprehensiveness. In summary, EcTracker provides an easy-to-perform, integrative and end-to-end single-cell data analysis platform that allows decoding of cellular identities, identification of ectopically expressed genes and their regulatory networks, and therefore, collectively imparts a novel dimension for analyzing single-cell datasets.
Evaluation of in silico tools for the prediction of protein and peptide aggregation on diverse datasets
Authors: Prabakaran R , Rawat Puneet , Kumar Sandeep , Gromiha M Michael 
Published: June 2021

Abstract
Several prediction algorithms and tools have been developed in the last two decades to predict protein and peptide aggregation. These in silico tools aid to predict the aggregation propensity and amyloidogenicity as well as the identification of aggregation-prone regions. Despite the immense interest in the field, it is of prime importance to systematically compare these algorithms for their performance. In this review, we have provided a rigorous performance analysis of nine prediction tools using a variety of assessments. The assessments were carried out on several non-redundant datasets ranging from hexapeptides to protein sequences as well as amyloidogenic antibody light chains to soluble protein sequences. Our analysis reveals the robustness of the current prediction tools and the scope for improvement in their predictive performances. Insights gained from this work provide critical guidance to the scientific community on advantages and limitations of different aggregation prediction methods and make informed decisions about their research needs.