bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stem cell transplantation therapy for diabetic foot ulcer: a narrative review
Authors: Shafiee Sahar , Heidarpour Maryam , Sabbagh Sima , Amini Elham , Saffari Hanieh , Dolati Sara , Meamar Rokhsareh 
Published: March 2021

Abstract
Diabetes mellitus is a chronic metabolic disease associated with high cardiovascular risk. A vascular complication of diabetes is foot ulcers. Diabetic foot ulcers are prevalent and substantially reduce the quality of life of patients who have them. Currently, diabetic foot ulcer is a major problem for wound care specialists, and its treatment requires considerable health care resources. So far, various therapeutic modalities have been proposed to treat diabetic foot ulcers and one of them is stem cell-based therapy. Stem cell-based therapy has shown great promise for the treatment of diabetic foot ulcers. This strategy has been shown to be safe and effective in both preclinical and clinical trials. In this review, we provide an overview of the stem cell types and possible beneficial effects of stem cell transplantation therapy for diabetic foot ulcers, and an overview of the current status of stem cell research in both preclinical and clinical trial stages of treatment strategies for diabetic foot ulcers.
Association of DRD2 (rs 1799732), ANKK1 (rs1800497), DAT (rs28363170), DRD4 (exon 3 - VNTR) gene polymorphisms in the context of relapses in therapy.
Authors: Chmielowiec Jolanta , Boroń Agnieszka 
Published: March 2021

Abstract

Introduction: Disorders in the field of reward system neurotransmission are mentioned as one of the most important causes of addiction. Genetic variation is assigned a special role. The literature on the subject mentions primarily the genes of dopamine neurotransmission: DAT (dopamine transporter), DRD2 (dopamine receptor D2), DRD4 (dopamine receptor D4). However, so far there are few literature reports on these genes in the context of innovators in addiction therapy.

The aim: Analysis of the relationship between the variability of specific polymorphisms in the DRD2 (rs1799732), ANKK1 (rs1800497), DAT (rs28363170), DRD4 (exon 3 - VNTR) genes with the occurrence of relapses in people addicted to psychoactive substances.

Material and methods: The research was carried out on a group of 301 people addicted to psychoactive substances staying in an addiction therapy center in Lubuskie and Zachodniopomorskie voivodships in Poland. The control group consisted of 301 people with no diagnosed addiction to psychoactive substances nor mental disorders. The study of polymorphisms DRD2 (rs 1799732), ANKK1 (rs1800497) was performed by real-time PCR method; whereas DAT (rs28363170), DRD4 (exon 3 - VNTR) was genotyped by PCR and the amplified products were visualized using ethidium bromide stained gel electrophoresis (3% agarose) and UV photography.

Results: This study showed that in addicts genotype frequencies of the VNTR polymorphism in the third exon of human DRD4 were as follow: S/L in 33.55%, S/S - 63.12% and L/L 3.32%; while in the control group S/L - 32.56%, S/S - 58.8 % and L/L - 8.6% (χ2 = 7.617, p = 0.022). Significant differences in the frequency of DRD2 gene polymorphism rs1799732 were observed (frequency of alleles; χ2 = 5.48, p = 0.0192) and DRD4 VNTR polymorphism (χ2 = 7.687, p = 0.021) between the addicted to psychoactive substances who have a one-time stay in an inpatient treatment center and the control group.
Understanding the Impact of COVID–19 on Global Financial Network Using Graph Based Algorithm: Minimum Spanning Tree Approach
Authors: Hatipoğlu Veysel Fuat 
Published: March 2021

Abstract
In this paper effects of COVID–19 pandemic on stock market network are analyzed by an application of operational research with a mathematical approach. For this purpose two minimum spanning trees for each time period namely before and during COVID–19 pandemic are constructed. Dynamic time warping algorithm is used to measure the similarity between each time series of the investigated stock markets. Then, clusters of investigated stock markets are constructed. Numerical values of the topology evaluation for each cluster and time period is computed.
Exploring wild edible flowers as a source of bioactive compounds: New perspectives in horticulture
Authors: Demasi Sonia , Caser Matteo , Donno Dario , Enri Simone Ravetto , Lonati Michele , Scariot Valentina 
Published: March 2021

Abstract
The increasing interest in healthy and natural foods has raised the attention towards uncommon or unexplored ingredients, such as edible flowers. These products are proven to be a rich source of bioactive compounds, for example, vitamins or polyphenols that play an important role in health promotion and disease prevention. However, plant species with edible flowers are numerous and most of them still need to be studied with this aim. The high species richness of North-Western Italy provides interesting perspectives in the use of wild edible flowers, which are currently underutilized, but can be a valuable food source or food supplement for healthy diets. In this framework, the phytochemical composition of 22 wild edible flowers was analysed and compared with that of four cultivated species (Borago officinalis L., Calendula officinalis L., Tagetes patula L. and Tropaeolum majus L.) to evaluate their potentiality as sources of bioactive compounds. The total polyphenol content (TPC) and antioxidant activity of the fresh flowers were assessed, together with their phenolic profiles and vitamin C content, through spectrophotometric and chromatographic analyses. The evaluated parameters varied widely among species, with Paeonia officinalis L. and Rosa pendulina L. showing the highest values of polyphenols (1,930 mg gallic acid equivalents (GAE) · 100 g−1 and 1,774 mg GAE · 100 g−1, respectively), followed by Rosa canina L. (1,397 mg GAE · 100 g−1) and Geranium sylvaticum L. (1,268 mg GAE · 100 g−1). The same species also showed the highest antioxidant activity, measured with three different assays [ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS)]. The phenolic profile differed among the studied species, with Dianthus pavonius Tausch and R. pendulina having the highest sum of detected polyphenols (2,522 mg · 100 g−1 and 2,366 mg · 100 g−1, respectively). Vitamin C was identified in all but two flowers (Allium ursinum L. and B. officinalis) and Primula veris L. had the highest amount (45 mg · 100 g−1). The study showed that wild edible flowers outperformed the cultivated species, except for T. majus, providing new insights for the use of wild edible flowers as sources of bioactive compounds.
Antiparasitic effects of selected isoflavones on flatworms
Authors: Faixová D. , Hrčková G. , Mačák Kubašková T. , Mudroňová D. 
Published: March 2021

Summary
Medicinal plants have been successfully used in the ethno medicine for a wide range of diseases since ancient times. The research on natural products has allowed the discovery of biologically relevant compounds inspired by plant secondary metabolites, what contributed to the development of many chemotherapeutic drugs. Flavonoids represent a group of therapeutically very effective plant secondary metabolites and selected molecules were shown to exert also antiparasitic activity. This work summarizes the recent knowledge generated within past three decades about potential parasitocidal activities of several flavonoids with different chemical structures, particularly on medically important flatworms such as Schistosoma spp., Fasciola spp., Echinococcus spp., Raillietina spp., and model cestode Mesocestoides vogae. Here we focus on curcumin, genistein, quercetin and silymarin complex of flavonolignans. All of them possess a whole spectrum of biological activities on eukaryotic cells which have multi-therapeutic effects in various diseases. In vitro they can induce profound alterations in the tegumental architecture and its functions as well as their activity can significantly modulate or damage worm´s metabolism directly by interaction with enzymes or signaling molecules in dose-dependent manner. Moreover, they seem to differentially regulate the RNA activity in numbers of worm´s genes. This review suggests that examined flavonoids and their derivates are promising molecules for antiparasitic drug research. Due to lack of toxicity, isoflavons could be used directly for therapy, or as adjuvant therapy for diseases caused by medically important cestodes and trematodes.
First report of Xiphinema diffusum from Pakistan
Authors: Shokoohi E. , Nasira K. , Iqbal E. , Hussain S. , W Mashela P. 
Published: March 2021

Summary
The population of Xiphinema americanum species group was detected from the soil surronding the roots of peach (Prunus persica) during a survey of plant-parasitic nematodes in Gilgit-Baltistan, Pakistan. From the results of the morphological study, the species was identified as X. diffusum. Morphometrics of X. diffusum generally agree with those of the type species and the topotype specimens. Molecular analysis of 18S rDNA of X. diffusum from Pakistan indicated two nucleotide differences and 99 % similarity with the Chinese (AM086669) and the Australian (AM086685) population of X. diffusum. Phylogenetic analysis of 18S rDNA placed the Pakistanis population close to X. diffusum from China and Australia. This species is reported for the first time from Pakistan
Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities
Authors: Khongkarat Phanthiwa , Ramadhan Rico , Phuwapraisirisan Preecha , Chanchao Chanpen 
Published: March 2021

Abstract
Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).
Monthly Changes in Honey Bee Forewings Estimated Using Geometric Morphometrics
Authors: Janczyk Anna , Tofilski Adam 
Published: March 2021

Abstract
Honey bee (Apis mellifera) wing measurements are often used to discriminate lineages and subspecies. The precision and repeatability of the measurements depends on various factors, including seasonal variation, and differences between left and right wings. The seasonal variation of the cubital index, which is the proportion of two vein lengths, has been investigated, but subspecies can be identified through geometric morphometrics. This method allows both wing size and shape to be determined and analyzed separately. We studied changes in the wing size and shape in consecutive months between June and September as well as the differences between the left and right wings. We found that wing size differed significantly among colonies, months and body sides, but shape differed significantly only among colonies and months but not among body sides. There was also a complicated pattern of interactions between various factors. The seasonal differences in wing shape were much smaller than the differences among colonies and their influence on identification of honey bee lineages was relatively small.
Nest Architecture and Nesting Site Preference of Tetragonula iridipennis Smith in North-Western Plains of India
Authors: Choudhary Amit , Singh Jaspal , Chhuneja Pardeep K. 
Published: March 2021

Abstract
Stingless bees constitute an important group of bee diversity providing a vital ecological service i.e. pollination. From India, nine species of stingless bees have been reported out of which Tetragonula iridipennis Smith is widely distributed. Studies revealed that the majority (86.67%) of these bees’ nests were present in brick walls and the remaining (13.33%) were in cavities of hollow tree trunks. Most of the colonies nested at a height of 3.50 m from the ground. Most of the nests (56.67%) had entrances oriented to the east. No distinct entrance tube was observed in 26.92 per cent of the colonies. Overall average entrance tube dimensions (longer side diameter × shorter side diameter × tube length) were 11.62 × 11.73 × 14.04 mm. In all the nests, brood area was surrounded by pollen pots and a few honey pots. The mean dimensions (depth × width) of brood cells, honey pots and pollen pots were 2.79 × 2.62 mm, 5.33 × 4.52 mm and 6.93 × 5.19 mm, respectively. All of the sixty colonies whether in brick/stone walls or in hollow tree trunks overwintered successfully. Our results can be useful for developing conservation measures for T. iridipennis and its augmentation for crop pollination.
Lone Geniuses or One among Many? An Explorative Study of Contemporary Highly Cited Researchers
Authors: Aksnes Dag W. , Aagaard Kaare 
Published: March 2021

Abstract

Purpose
The ranking lists of highly cited researchers receive much public attention. In common interpretations, highly cited researchers are perceived to have made extraordinary contributions to science. Thus, the metrics of highly cited researchers are often linked to notions of breakthroughs, scientific excellence, and lone geniuses.

Design/methodology/approach
In this study, we analyze a sample of individuals who appear on Clarivate Analytics’ Highly Cited Researchers list. The main purpose is to juxtapose the characteristics of their research performance against the claim that the list captures a small fraction of the researcher population that contributes disproportionately to extending the frontier and gaining—on behalf of society—knowledge and innovations that make the world healthier, richer, sustainable, and more secure.

Findings
The study reveals that the highly cited articles of the selected individuals generally have a very large number of authors. Thus, these papers seldom represent individual contributions but rather are the result of large collective research efforts conducted in research consortia. This challenges the common perception of highly cited researchers as individual geniuses who can be singled out for their extraordinary contributions. Moreover, the study indicates that a few of the individuals have not even contributed to highly cited original research but rather to reviews or clinical guidelines. Finally, the large number of authors of the papers implies that the ranking list is very sensitive to the specific method used for allocating papers and citations to individuals. In the “whole count” methodology applied by Clarivate Analytics, each author gets full credit of the papers regardless of the number of additional co-authors. The study shows that the ranking list would look very different using an alternative fractionalised methodology.

Research limitations
The study is based on a limited part of the total population of highly cited researchers.

Practical implications
It is concluded that “excellence” understood as highly cited encompasses very different types of research and researchers of which many do not fit with dominant preconceptions.

Originality/value
The study develops further knowledge on highly cited researchers, addressing questions such as who becomes highly cited and the type of research that benefits by defining excellence in terms of citation scores and specific counting methods.
A coupled impact of different management and soil moisture on yield of winter wheat (Triticum aestivum L.) in dry conditions at locality Mezőföld, Hungary
Authors: Milics Gabor 
Published: March 2021

Abstract
Variable rate technology (VRT) in nutrient management has been developed in order to apply crop inputs according to the required amount of fertilizers. Meteorological conditions rarely differ within one field; however, differences in soil conditions responding to precipitation or evaporation results within field variations. These variations in soil properties such as moisture content, evapotranspiration ability, etc. requires site-specific treatments for the produced crops. There is an ongoing debate among experts on how to define management zones as well as how to define the required amount of fertilizers for phosphorus and nitrogen replenishment for winter wheat (Triticum aestivum L.) production. For management zone delineation, vegetation based or soil based data collection is applied, where various sensor technology or remote sensing is in help for the farmers. The objective of the study reported in this paper was to investigate the effect of soil moisture data derived from Sentinel-2 satellite images moisture index and variable rate phosphorus and nitrogen fertilizer by means of variable rate application (VRA) in winter wheat in Mezőföld, Hungary. Satellite based moisture index variance at the time of sowing has been derived, calculated and later used for data comparison. Data for selected points showed strong correlation (R2 = 0.8056; n = 6) between moisture index and yield, however generally for the whole field correlation does not appear. Vegetation monitoring has been carried out by means of NDVI data calculation. On the field level, as indicated earlier neither moisture index values at sowing nor vegetation index data was sufficient to determine yield. Winter wheat production based on VRA treatment resulted significant increase in harvested crop: 5.07 t/h in 2013 compared to 8.9 t/ha in 2018. Uniformly managed (control) areas provided similar yield as VRA treated areas (8.82 and 8.9 t/ha, respectively); however, the input fertilizer was reduced by 108 kg/ha N and increased by 37 kg/ha P.
The effects of rainfall characteristics and land use and cover change on runoff in the Yellow River basin, China
Authors: Hu CaiHong , Ran Guang , Li Gang , Yu Yun , Wu Qiang , Yan Denghua , Jian Shengqi 
Published: March 2021

Abstract
The changes of runoff in the middle reaches of the Yellow River basin of China have received considerable attention owing to their sharply decline during recent decades. In this paper, the impacts of rainfall characteristics and land use and cover change on water yields in the Jingle sub-basin of the middle reaches of the Yellow River basin were investigated using a combination of statistical analysis and hydrological simulations. The Levenberg Marquardt and Analysis of Variance methods were used to construct multivariate, nonlinear, model equations between runoff coefficient and rainfall intensity and vegetation coverage. The land use changes from 1971 to 2017 were ascertained using transition matrix analysis. The impact of land use on water yields was estimated using the M-EIES hydrological model. The results show that the runoff during flood season (July to September) decreased significantly after 2000, whereas slightly decreasing trend was detected for precipitation. Furthermore, there were increase in short, intense, rainfall events after 2000 and this rainfall events were more conducive to flood generation. The “Grain for Green” project was carried out in 1999, and the land use in the middle reaches of the Yellow River improved significantly, which make the vegetation coverage (Vc
) of the Jingle sub-basin increased by 13%. When Vc
approaches 48%, the runoff coefficient decreased to the lowest, and the vegetation conditions have the greatest effect on reducing runoff. Both land use and climate can change the water yield in the basin, but for areas where land use has significantly improved, the impact of land use change on water yield plays a dominant role. The results acquired in this study provide a useful reference for water resources planning and soil and water conservation in the erodible areas of the middle reaches of the Yellow River basin.
Age and seasonal pattern of contagious agalactia in small ruminants in Ukraine
Authors: Bohach Dmytro Mykolaivych , Stegniy Borys Tymofiyovych , Bohach Mykola Volodymyrovych , Pavlov Serhii Leonidovych , Bolotin Vitaliy Igorovych 
Published: March 2021

Abstract

Introduction
The aim of the study was to determine how the spread of contagious agalactia in sheep and goats in the Odesa region depended on the age of the animals and the season.

Material and Methods
From January 2016 to December 2018, 1,964 ewes and 1,484 nanny goats of different age groups were studied by ELISA for antibodies to Mycoplasma agalactiae.

Results
The highest incidence of contagious agalactia was registered in one-year-old animals and was 59.7‒83.0%, two-year-old ruminants showed 17.0‒40.3% prevalence, in livestock at the age of 3–4 years no serological evidence of the disease was registered and in ewes and nanny goats older than 5–6 years 1.5–3.6% were infected. The most susceptible were young animals at the age of one-month (11.6‒14.5%). The first peak of the disease was recorded in March‒April (21.0‒26.1%), in the lambing period, which coincided with the beginning of lactation and the suckling period, and the second peak occurred in June–July (28.9‒34.2%), the period of maximum lactation and of manual milking of sheep and goats.

Conclusion
The results of serological investigations indicate the circulation of M. agalactiae in small ruminants in the south of Ukraine. To avoid greater dissemination of the pathogen, appropriate measures should be applied and strategies for its control need to be drawn up.
Comparative Study on Micromechanical Properties of ZnO:Ga and ZnO:In Luminiscent Ceramics
Authors: Muktepavela F. , Zolotarjovs A. , Zabels R. , Kundzins K. , Gorokhova E. , Tamanis E. 
Published: March 2021

Abstract
Indium (0.038 at.%) and gallium (0.042 at.%) doped ZnO ceramics were prepared by hot pressing. Ceramics were investigated to determine their structural and mechanical characteristics for the prospective use in scintillators. Based on results of nanoindentation, atom force and scanning electron microscopy as well as energy dispersive X-ray spectra measurements, locations of gallium within grain, indium at grain boundaries (GBs) and their different effect on the mechanical properties of ZnO ceramics were detected. Doping of gallium led to the increased modulus of elasticity in grain, decreased hardness near GBs, stabilization of micropores and brittle intercrystalline fracture mode. ZnO:In ceramic has modulus of elasticity and hardness values close to ZnO characteristics, the increased fracture toughness and some plasticity near GBs. Differences in the micromechanical properties of the ceramics correlate with the location of dopants. Results demonstrate that the ZnO:In ceramic has a greater stress relaxation potential than the ZnO:Ga.