bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selected ‘Starter Kit’ energy system modelling data for Ecuador (#CCG)
Authors: Cannone Carla , Allington Lucy , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Howells Mark , Walker Miriam Zachau , Ahsan Aniq , Charbonnier Flora , Halloran Claire , Hirmer Stephanie , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Rogner Holger , Quirós-Tortós Jairo , Angulo-Paniagua Jam 
Published: September 2021

Abstract
Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to energy system modelling, causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Ecuador, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020–2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
Effect of Fractions from Lycopus lucidus Turcz. Leaves on Genomic DNA
Oxidation and Matrix Metalloproteinase Activity
Authors: Lim Sun Young , Chen Jingwen , Na Eun 
Published: September 2021

Aim and Objective:
We investigated the inhibitory effects of fractions from Lycopus
lucidus Turcz. leaves on genomic DNA oxidation, Nitric Oxide (NO) production, and Matrix
Metalloproteinase (MMP) activity.

Material and Methods:
Oxidative damage of genomic DNA was detected after Fenton reaction with
H2O2 using DNA electrophoresis. Western blotting was performed to compare the expression levels
of MMP-2 in phorbol 12-myristate 13-acetate (PMA)-induced HT-1080 cells. Lipopolysacchride
(LPS)-induced NO production in RAW 264.7 cells was measured using Griess reagent.

Results:
All fractions (n-Hexane, 85% aq. MeOH, n-BuOH, and water fractions) from the leaves of
L. lucidus Turcz. significantly inhibited intracellular production of reactive oxygen species (ROS)
(p<0.05). Particularly, 85% aq. MeOH and n-BuOH fractions showed higher ROS inhibitory
activity than the other fractions. n-Hexane, 85% aq. MeOH, n-BuOH and water (0.05 mg/mL)
fractions significantly inhibited oxidative DNA damage by 57.97%, 68.48%, 58.97%, and 68.39%,
respectively (p <0.05). Treatment of RAW 264.7 cells with each fraction reduced LPS-induced NO
production in a dose-dependent manner (p<0.05). n-Hexane and 85% aq. MeOH fractions notably
reduced MMP-2 secretion levels in the culture supernatants from HT-1080 cells.

Conclusion:
Overall, these results indicated that L. lucidus Turcz. leaves can be exploited as plant
based sources of antioxidants in the pharmaceutical, cosmetic, nutraceutical, and food industries.
Factors Promoting Alkene Migratory Insertion into Metal-nitrogen Bond
During Hydroamination in Recent Studies
Authors: Han Wenlai 
Published: September 2021

:
Migratory insertion is a fundamental organometallic transformation that enables the functionalization
of an unsaturated bond. Recent reports on catalytic hydroamination provide evidence
that supports an intermolecular migratory insertion pathway featuring alkene insertion into metalnitrogen
(M-N) bonds. This article presents factors influencing the rate of migratory insertion in latetransition
metal-catalyzed hydroamination, including steric and electronic effects from ligands, alkenes,
and metal centers, along with stabilization from coordinated amine intermediates and ordered
transition states.
Adverse Drug Reactions of Acetaminophen and Ibuprofen in the Paediatric Population: Analysis of the Italian Spontaneous Reporting Database
Authors: Pelliccia Valentina , Rossi Serena , Zollino Ilaria , Quagliarella Francesco , Buonocore Giuseppe 
Published: September 2021

Background:
Acetaminophen and ibuprofen are the only antipyretics drugs approved in
children, and are considered safe and well tolerated. However, data regarding the adverse drug reaction
(ADR) profile of these drugs in children are scattered.

Aim:
The aim of our study is to evaluate the ADRs of acetaminophen and ibuprofen through an observational
study over a period of 15 years (January 2005-April 2020). Reports of suspected ADRs
to the active substances ‘acetaminophen’ and ‘ibuprofen’ are listed and accessible through the
Italian spontaneous reporting database (RAM system) by AIFA (Pharmacovigilance of the Italian
Drug Agency).

Methods:
Acetaminophen ADRs in paediatric populations were 15% of cases, with more frequent
involvement of skin and soft tissue (54.36%) and gastrointestinal apparatus (44.09%); liver dysfunction
accounts for 5.67%.

Results:
Ibuprofen paediatric ADRs were 26%: skin and soft tissues in 63.16% of cases, gastrointestinal
tract in 47.75%, hematemesis and melena in 6.38%; kidney injury in 2.25% of cases.

Conclusion:
Children aged 2 to 11 are more frequently affected by ADRs than infants and adolescents.
The risk of gastrointestinal and renal side effects is significantly higher with ibuprofen. Hepatobiliary
side effects are more frequently linked to acetaminophen. Potentially fatal ADRs have
been reported sporadically for both drugs.
Protective Effects of Omega-3 Fatty Acids Supplementation Against Renal Parenchymal Scarring in Children with Acute Pyelonephritis: Results of a Pilot Clinical Trial
Authors: Sedighi Iraj , Taheri-Moghadam Ghazal , Emad-Momtaz Hossein , Vaseghi Golnaz , Eshraghi Azadeh , Asnaashari Farzaneh , Mehrpooya Maryam 
Published: September 2021

Aim::
This trial aimed to determine if supplementation with omega-3 fatty acids as an adjunct
therapy to antibiotic treatment can have protective effects against renal scar formation after
acute pyelonephritis (APN) in pediatric patients.

Background:
Current evidence points out that besides antibiotic treatment, early administration of
antioxidant and anti-inflammatory compounds may be effective in reducing the occurrence of renal
damage following APN in children.

Objective:
The main endpoint of the trial was the comparison of the development of renal scarring
formation after APN in an omega-3 fatty acids-treated group and in a control-treated group.

Methods:
This prospective randomized, controlled trial study was conducted from March 2016 to
May 2018 on 60 children with a diagnosis APN in a tertiary hospital in Iran. After the diagnosis of
APN based on the clinical signs and symptoms, urine analysis, urine culture, and dimercaptosuccinic
acid renal scan (DMSA scan), the patients were randomly allocated into either the control
group (n=30 patients: received standard antibiotic treatment only) or the intervention group (n=30
patients: received standard antibiotic-treatment in combination with oral omega-3 fatty acids based
on the children’s weight for three consecutive days). A second DMSA scan was performed for the
patients at a minimum of six months after treatment. The development of renal scars was evaluated
by comparing the baseline DMSA scan lesions with the follow-up DMSA scan lesions.

Results:
Fifty patients, including 26 and 24 individuals in the control and intervention groups, respectively,
completed the entire course of the study. Renal parenchymal involvement based on the
baseline DMSA scan was similar in the two groups (p-value =0.85, 0.90, and 0.53 regarding the
right, left, and both kidney units together, respectively). Although comparison of the follow-up
DMSA scan lesions to the baseline DMSA scan lesions considering the right and left kidneys as separate
units between two groups did not reach the significant level, when considering both left and
right kidney units together, results showed a statistically significant difference between groups in
favor of the intervention group (p-value =0.04).

Conclusions:
Although preliminary, the results of this study showed that administration of omega-3
fatty acids, a natural supplement with well-known anti-inflammatory and antioxidant properties, as
an adjunct therapy to standard antibiotic treatment might significantly reduce the incidence of the
occurrence renal scarring following APN in children. Confirmation of these results requires further
studies.

Clinical Trial Registeration:
This trial was registered to Iranian Clinical Trials Registry with the
trial number IRCT2015062922965N1.
Recent Advances in Nanomaterial-based Luminescent ATP Sensors
Authors: Zhou Xiaomeng , Shang Li 
Published: September 2021

:
Adenosine 5'-triphosphate (ATP) plays a significant role in biological processes and the
ATP level is closely associated with many diseases. In order to detect ATP in live cells, tissues, and
body fluids with high sensitivity and selectivity, researchers have developed various sensing strategies.
Particularly, owing to the distinct physicochemical properties of nanomaterials and the high
sensitivity of fluorescence, a lot of efforts have been devoted to developing nanomaterials-based
approaches for fluorescent ATP sensing in recent years. In this review, we focus on the current
development of nanomaterial-based fluorescent ATP sensors and discuss the sensing mechanisms
in detail. The advantages and disadvantages of ATP sensing using different kinds of nanomaterials,
including carbon nanomaterials, metal nanoparticles, semiconductor quantum dots, metal-organic
frameworks, and up-conversion nanoparticles have been thoroughly compared and discussed. Finally,
current challenges and future prospects in this field are examined.
Anticancer Effect and Phytochemical Profile of the Extract from Achillea ketenoglui
against Human Colorectal Cancer Cell Lines
Authors: Ayan İlknur Ç. , Çetinkaya Sümeyra , Dursun Hatice G. , Güneş Canan E. , Şirin Seda 
Published: September 2021

Background:
In the treatment of Colorectal Cancer (CRC), the search for new antineoplastic drugs with
fewer side effects and more effectiveness continues. A significant part of these pursuits and efforts focus on medicinal
herbs and plant components derived from these plants. A. ketenoglui is one of these medicinal plants, and its anticancer
potential has never been studied before.

Methods:
The phenolic and flavonoid content, and antioxidant activity of A. ketenoglui extracts were determined. The
phytochemical profiling and quantification analysis of major components were performed by HPLC-ESI-Q-TOF-MS.
Cytotoxicity, proliferation, apoptosis and cell cycle were evaluated to reveal the anticancer activity of the extract on
CRC cells (HCT 116 and HT-29). The determined anticancer activity was confirmed by mRNA (RT-qPCR) and protein
(Western blotting) analyzes.

Results:
A. ketenoglui methanol extract was found to have high phenolic (281.89±0.23) and flavonoid (33.80±0.15)
content and antioxidant activity (IC50 40.03±0.38). According to the XTT assay, the extract has strong cytotoxic activity
(IC50 350 μM in HCT 116 and IC50 263 μM in HT-29 cell line). The compounds most commonly found in the plant
are, in descending order, chlorogenic acid, apigenin, genistin, baicalin, eupatorin, casticin, and luteolin. In flowcytometric
analysis, the extract was found to induce greater apoptosis and cell cycle arrest in both cell lines than in both
control and positive control (casticin). According to the results of the mRNA expression analysis, the extract treatment
upregulated the expression of the critical genes of the cell cycle and apoptosis, such as p53, p21, caspase-3, and
caspase-9. In protein expression analysis, an increase in caspase-3 and p53 expression was observed in both cell lines
treated with the extract. In addition, caspase-9 expression was increased in HT-29 cells.

Conclusion:
The findings show that A. ketenoglui has an anticancer potential by inducing apoptosis and arresting the
cancer cell cycle and may be promising for CRC therapy. This potential of the plant is realized through the synergistic
effects of its newly identified components.
Thyroid Function: A Target for Endocrine Disruptors, Air Pollution and Radiofrequencies
Authors: Di Ciaula Agostino , Bonfrate Leonilde , Noviello Marica , Portincasa Piero 
Published: September 2021

Abstract:Thyroid diseases, including congenital hypothyroidism, thyroiditis, and childhood thyrotoxicosis, are progressively increasing. The incidence of thyroid cancer in children and adolescents has also increased in recent decades, mirroring the trends observed in adults. These epidemiologic trends develop in parallel with the rising costs associated with diagnosis and treatment of thyroid diseases. Both genetic and environmental factors are involved in these diseases, and a number of widely diffused toxic chemicals of anthropogenic origin can impair thyroid function and make thyroid cancer worse. Synthetic substances persistently contaminate environmental matrices (i.e., air, soil, water) and the food chain and bio-accumulate in humans, starting from in utero life. Environmental toxins such as air pollutants, endocrine disruptors, and high-frequency electromagnetic fields can act on common targets through common pathways, combined mechanisms, and with trans-generational effects, all of which contribute to thyroid damage. Both experimental and epidemiologic observations show that mechanisms of damage include: modulation of synthesis; transportation and metabolism of thyroid hormones; direct interference with hormone receptors: modulation of gene expression; and autoimmunity. We should not underestimate the available evidence linking environmental pollutants with thyroid disease, cancer included, since toxic substances increasingly diffuse and thyroid hormones play a key role in maintaining systemic metabolic homeostasis during body development. Thus, primary prevention measures are urgently needed in particular to protect children, the most exposed and vulnerable subjects.
Nitric Oxide Modulation as a Potential Molecular Mechanism Underlying
the Protective Role of NaHS in Liver Ischemia Reperfusion Injury
Authors: Ibrahim Salwa A. , Abdel-Gaber Seham A. , Ibrahim Mohamed A. , Amin Entesar F. , Mohammed Rehab K. , Abdelrahman Aly M. 
Published: September 2021

Background and aim:
Liver IR is a frequent clinical complication with high morbidity
and mortality. The present study evaluated the possible protective effect of sodium hydrosulfide
(NaHS), a H2S donor, in IR-induced hepatic injury and explored the mechanisms of actions of the
investigated drug.

Methods:
Male albino rats (200-230 g) were divided into the following groups: group 1:Sham-operated
non treated rats, group 2: IR non treated rats, group 3: L-NNA + IR rats, group 4: NaHS + IR
rats, group 5: L-NNA + NaHS + IR rats. Blood samples were collected for ALT determination. Liver
tissue samples were used for the assessment of GPx, catalase, SOD, MDA, total nitrites and TNF-
α. Parts from the liver were fixed in 10% formalin solution for histopathological examination
and immunohistochemical examination of iNOS, eNOS and caspase-3.

Results:
NaHS protected the liver against IR. This hepatoprotection was associated with normalization
of antioxidant enzyme activity and decrease in hepatic MDA, TNF-α and expression of caspase-
3 and iNOS.

Conclusion:
NaHS is hepatoprotective in IR injury. The hepatoprotective effects of NaHS are associated
with antioxidant, anti-inflammatory and antiapoptotic effects. These effects are probably mediated
via NO modulation.
In-Silico Analysis of rSNPs in miRNA:mRNA Duplex Involved in Insulin
Signaling Genes Shows a Possible Pathogenesis of Insulin Resistance
Authors: Sunny Jithin S. , Saleena Lilly M. 
Published: September 2021

Background:
Insulin resistance is a condition in which the body produces insulin but is
unable to use it effectively. Aberrations in insulin signaling are known to play a crucial role in the
pathogenesis of this disease state. Eventually, patients will have glucose build-up in their blood instead
of being absorbed by the cells, leading to type 2 diabetes.

Objective:
In the current study, we focus on understanding the role of rSNP mediated miRNA:mRNA
dysregulation and its impact on the above metabolic condition.

Methods:
More than 30 genes involved in the insulin signaling pathway were found using the
KEGG database. The 3’UTR end of genes was studied by using RegRNA and Ensembl, whereas
TargetScan along with miRbase were used to identify their target miRNAs. Binding free energy
was used as a parameter to analyze the effect of polymorphism on the miRNA:mRNA duplex formation.
Further, the UNA fold was used to determine the heat capacity changes.

Results:
The genes INSR, INS, GLUT4, FOXO1, IL6, TRIB3, and SREBF1, were selected for
analysis. Multiple miRNAs, hsa-miR-16-5p, hsa-miR-15a-15p were identified in the SNP occurring
region for INSR. INS, too, showed similar results. INSR, INS, and TRIB3 were found to have
the maximum change in their binding free energy due to rSNP variation. A destabilisation in the
heat capacity values was observed too, which contributed due to rSNP induction.

Conclusion:
A direct relationship between miRNA target polymorphism and the stability of the
miRNA:mRNA duplex was observed. The current methodology used to study insulin resistance
pathogenesis could elaborate on our existing knowledge of miRNA-mediated disease states.
Regeneration Potential of Bone Marrow-derived Mesenchymal Stromal
Cells Lysate for H2O2 (In-Vitro) Injured Cells
Authors: Malik Sabeen , Awan Sana Javaid , Hashim Mariam , Farzand Anam , Nadeem Shumaila 
Published: September 2021

Background:
Mesenchymal stromal cells (MSCs) and their paracrine factors make them
a suitable cell-free-therapeutic candidate. Cellular lysate usage could be an effective treatment strategy
that circumvents the possible associated drawbacks of stem cell therapies.

Objective:
Thus, this research aims to examine the injury regeneration potential of MSCs cellular
lysate derived from bone marrow by studying its anti-apoptotic, proliferative, and anti-oxidative effects.

Methods:
Hydrogen peroxide (H2O2) was used to induce cellular injury. MTT assay, trypan blue,
and crystal violet assays were used to assess bone marrow-derived mesenchymal stromal cell (BMSCs)
lysate treated cells' viabilities. Next, to investigate the BMSCs lysate anti-oxidative potential
anti-oxidants, ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide dismutase
(SOD) assays were performed. Simultaneously, the proliferative and anti-apoptotic potential was
measured via vascular endothelial growth factor (VEGF A) and p53 expression analysis through
immunostaining and ELISA.

Results:
It was observed that BMSCs lysate enhances the viability of H2O2 injured cells. APX, GR,
and SOD's results indicated that after H2O2 injury, the anti-oxidant status decreased significantly
and was uplifted by BMSCs lysate treatment. Additionally, the results of p53, BAX, and caspase-3
expression revealed that BMSCs lysate inhibits apoptosis by downregulating their expression in
treated cells. The VEGF protein expression findings demonstrated that BMSCs lysate upregulates
the downregulated expression of VEGF in H2O2 injured cells. The expression of proliferative markers
(TOP2A, PCNA, and Ki-67) was also elevated in BMSCs treated cells.

Conclusion:
To conclude this study's findings, it was observed that BMSCs lysate could decrease
H2O2 injury and possibly regenerate the injured cells by enhancing their viability and proliferation,
improving anti-oxidants levels, and alleviating apoptosis.
Local adaptation of invasive plant, Synedrella nodiflora, in urban tropical lowland landscape Universitas Indonesia
Authors: Maryanto Andi Eko , Salamah Andi , Windarti Citra Karina , Syadewi Mutia 
Published: September 2021

Synedrella nodiflora is an invasive species originated from tropical America and now has spread throughout Indonesia. We analysed the ability of Synedrella nodiflora from the level of HSP70 gene expression at different heat stress in urban tropical lowland landscape Universitas Indonesia. We used the qPCR to quantify the level of HSP70 gene expression and analysed using Pfaffl model. We found the level of HSP70 gene expression got higher related to elevated temperature from 29oC to 39oC with a range of fold from 123.1 to 1676.9. This ability reflects the adaptive plasticity of Synedrella nodiflora in the course of the invasion process.
AMELIORATIVE ROLE OF GARLIC (ALLIUM SATIVUM) ON CHROMIUM (VI) - INDUCED MEMBRANE DAMAGE IN MALE ALBINO RATS
Authors: SOUMITA DEY  , SANKAR KUMAR DEY  
Published: September 2021

Objective: Membrane damage is one of the important consequences of chromium (Cr) induced cytotoxicity. Garlic possesses antioxidant property to scavenge the toxic radicals and cytoprotective activity. The aim of the present study is to evaluate the ameliorative role of aqueous extract of garlic (AEG) on Cr-induced membrane damage of both liver and kidneys in male albino rats.
Methods: Male albino rats of Wistar strain (80-100 g) were used for the present study. Rats were divided into three groups of almost equal average body weight. The animals of two groups were injected K2Cr2O7 at a dose of 0.8 mg per 100 g body weight per day for 28 days. The animals of one of the Cr-treated groups served as the supplemented group supplied aqueous extract of garlic (AEG) (250 mg per kg body weight daily at an interval of 6 h after injection of Cr for a period of 28 days). The animals of the remaining group received only the vehicle (0.9% NaCl), served as control. The body weights of the animals were taken in each day of treatment schedule.
Results: The results indicated that significant increases in membrane cholesterol level as well as significant decreases in membrane phospholipid level in Cr exposed animals suggest structural alterations in both liver and kidneys plasma membrane. Alkaline phosphatase (ALP), total ATPase, and Na+-K+- ATPase activities of plasma membrane were significantly decreased in both liver and kidneys after Cr treatment. On the other hand, AEG supplementation plays a vital role to restore such alterations induced by Cr in plasma membrane of both liver and kidney.
Conclusion: These findings indicate that Cr treatment at the present dose and duration induces structural and functional alterations in the plasma membrane in both liver and kidney. However, AEG supplementation restored those alterations induced by Cr in plasma membrane of both liver and kidneys but was not able to eliminate the deposited Cr from the liver and kidney tissues.