bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a new 18 X‐STR typing assay for forensic applications
Authors: Zhang Yinming , Yu Zhengliang , Mo Xiaoting , Zhao Xingchun , Li Wanshui , Liu Hong , Liu Chao , Wu Riga , Sun Hongyu 
Published: January 2021

AbstractWith a unique inheritance pattern compared to autosomal short tandem repeats (A‐STRs), X chromosomal STRs (X‐STRs) have special usage in forensic relationship testing. In this study, we designed a multiplex amplification system (named TYPER‐X19 multiplex assay) consisting of 18 STR loci spreading from 7.837 to 149.460 Mb on the X chromosomes (DXS9895, DXS8378, DXS9902, DXS6810, DXS7132, DXS10079, DXS6789, DXS7424, DXS101, DXS6797, DXS7133, DXS6804, GATA165B12, DXS10103, HPRTB, GATA31E08, DXS8377, and DXS7423), and the amelogenin. PCR primers were marked with four kinds of fluorophores including FAM, HEX, TAMRA, and ROX. The multiplex system was optimized and tested for precision, concordance, reproducibility, sensitivity, stability, DNA mixture, and species specificity according to the conventional validation guidelines. The results indicated that the system was accurate, reliable, and sensitive enough, and was suitable for common forensic case‐type samples. In the population genetic study, a total of 148 alleles were detected at the 18 X‐STR loci in 398 Southern Han Chinese. Relatively high combined power of discrimination in male (PDm), power of discrimination in female (PDf), mean paternity exclusion chance in trios (MECtrio), and mean paternity exclusion chance in duos (MECDuo) by Desmarais were detected, and HPRTB‐DXS10103 was in linkage disequilibrium. The results suggested that the TYPER‐X19 multiplex assay was suitable for forensic applications.
Characterization of the microbiota and chemical properties of pork loins during dry aging
Authors: Endo Akihito , Koizumi Ryosuke , Nakazawa Yozo , Shiwa Yuh , Maeno Shintaro , Kido Yoshihiko , Irisawa Tomohiro , Muramatsu Yoshiki , Tada Kotaro , Yamazaki Masao , Myoda Takao 
Published: January 2021

AbstractDry aging (DA) allows for the storage of meat without packaging at 0 to 3°C for several weeks. It enhances the production of pleasant flavors, tenderness, and juiciness in meat. Due to the long storage period and roles of indigenous microbiota in the maturation of several meat products, the microbiota of DA meat is of interest in terms of microbial contributions and food hygiene but has not yet been characterized in detail. This study identified the microbiota of pork loins during DA using culturing and culture‐independent meta‐16S rRNA gene sequencing and elucidated its characteristics. The amounts of free amino acids and profiles of aroma‐active compounds were also monitored by high‐performance liquid chromatography and gas chromatography, respectively. The meta‐16S rRNA gene sequencing revealed that Pseudomonas spp. generally dominated the microbiota throughout DA; however, the culturing analysis showed marked changes in the species composition during DA. Acinetobacter spp. were the second most dominant bacteria before DA in the culture‐independent analysis but became a minor population during DA. The cell numbers of yeasts showed an increased tendency during DA, and Debaryomyces hansenii was the only microorganism isolated from all meat samples throughout DA. Well‐known foodborne pathogens were not observed in two microbiota analyses. The amounts of free amino acids were increased by DA, and the number of aroma‐active compounds and their flavor dilution values markedly changed during DA. Most microbial isolates showed positive reactions with proteolytic and lipolytic activities, suggesting their contribution to tenderness and aroma production in DA meats.
Toward achieving sustainable development goal 3: Determinants, innovations, and reactions from 110 countries with different income levels
Authors: Fu Guoxin 
Published: January 2021

AbstractWell‐being has a major impact on sustained development of economies worldwide; and unionized well‐being‐improving decisions are key to achieving SDG3, but research on such is rare. This article investigates the determinants of well‐being using Life Ladder (LLD), log GDP per capita (LGDP), social support (SSP), and life expectancy at birth (HLE) for panels of 110 countries selected from World Happiness Report 2020, and grouped according to income levels. The empirical panel Granger causality results show that LGDP, SSP and HLE Granger causes LLD in the long‐run for low, upper‐middle, and high‐income countries; but find no evidence of such for the Lower‐Middle Income Countries and Global Panel. Evidence from the impulse response and variance decomposition analysis shows that majority of the innovations needed to promote well‐being can be found within LLD itself. The policy implication is that though all countries plan to improve well‐being, the determinants, innovations, and corresponding reactions vary across countries.
Dichrostachys cinerea Growth Rings as Natural Archives for Climatic Variation in Namibia
Authors: Mapani Benjamin , Shikangalah Rosemary , Mapaure Isaac , Musimba Aansbert 
Published: January 2021

AbstractGlobal Circulation Models (GCMs) are used to forecast climate change in Southern Africa, and the evidence shows that the region is going to warm up by up to 2° by the year 2050. Namibia is one of the driest countries in Southern Africa and is at a high risk of becoming much drier than current situation by 57%. Very few studies have been carried out in Southern Africa to show actual impacts of climate change. Practical applicability of GCMs at a local spatial scale remains limited due to the coarse nature of the models. Hence, improvement of the GCMs must begin with better understanding of the local microclimates and how they respond to regional circulation patterns. In many regions of Southern Africa, the lack of potential tools to access old climatic records precludes the estimation of climate trends beyond 100 years. In spite of these impediments, there are areas with excellent tree species such as Dichrostachys cinerea that are able to be used as climatic archives for specific time periods. In this chapter, the study shows that the combination of tree ring chronologies and precipitation records is a powerful methodology in climate modeling in the southern hemisphere and reveals nuances that show climate change. The evaluation of data from tree rings coupled with precipitation trends reveals signals that show that climate has indeed been changing over the past ten decades and will have a negative impact on livelihoods. These data can now be used in predictive models that can be used to project future scenarios and assist policy makers and planners to see how climate will evolve in the next 50–60 years.
Effects of shot peening and artificial surface defects on fatigue properties of 50CrV4 steel
Authors: Saklakoglu Nursen , Bolouri Amir , Irizalp Simge Gencalp , Baris Fatih , Elmas Ali 
Published: January 2021

AbstractShot peening processes are commonly used for improving the fatigue properties of steels. Shot peening introduces a compressive residual stress field in the near surface of steel, which can reduce or stop the growth of fatigue cracks and improve fatigue properties. This study experimentally investigated the effect of shot peening on the fatigue properties of 50CrV4 steel alloys with different artificial surface defects. Drilling tools were used to introduce different artificial defects with root radii of 0.585 mm and 0.895 mm on the surface of unpeened samples. The shot peening was applied to the drilled and undrilled samples. Scanning electron microscopy (SEM) observations, micro-hardness and X-ray diffraction residual stress measurements were conducted to analyse the characteristics of the shot-peened and unpeened samples. The results show that the shot peening leads to the transformation of the retained austenite to martensite in the near-surface microstructure. The hardness rates of the surface and near surface both increase by 8% after the shot peening. The peened samples exhibit compressive residual stresses with a high degree of isotropy in the near surface. The fatigue properties of samples were experimentally evaluated by conducting 3-point bending tests. The results indicate that the shot peening improves the fatigue life of drilled and undrilled samples. For the defects with the root radius of 0.895 mm, the shot peening leads to a 500% improvement in the fatigue life compared to unpeened samples regardless of defect depth. For the defects with the root radius of 0.585 mm, the improvement in fatigue life is 40% for the defect depth of 0.2 mm compared to unpeened samples. The improvement increases to 60% and 200% by increasing the defect depths to 0.4 mm and 0.6 mm. The fatigue properties are linked to the changes in the features of defects mainly caused by the deformation hardening and compressive residual stress after shot peening.
Cylindrically and non-cylindrically symmetric expansion dynamics of tin microdroplets after ultrashort laser pulse impact
Authors: de Faria Pinto Tiago , Mathijssen Jan , Meijer Randy , Zhang Hao , Bayerle Alex , Kurilovich Dmitry , Versolato Oscar O. , Eikema Kjeld S. E. , Witte Stefan 
Published: January 2021

AbstractIn this work, the expansion dynamics of liquid tin micro-droplets irradiated by femtosecond laser pulses were investigated. The effects of laser pulse duration, energy, and polarization on ablation, cavitation, and spallation dynamics were studied using laser pulse durations ranging from 220 fs to 10 ps, with energies ranging from 1 to 5 mJ, for micro-droplets with an initial radius of 15 and 23 $$\upmu$$μm. Using linearly polarized laser pulses, cylindrically asymmetric shock waves were produced, leading to novel non-symmetric target shapes, the asymmetry of which was studied as a function of laser pulse parameters and droplet size. A good qualitative agreement was obtained between smoothed-particle hydrodynamics simulations and high-resolution stroboscopic experimental data of the droplet deformation dynamics.
Impact of prescription drug monitoring program mandate on postoperative opioid prescriptions in children
Authors: Theodorou Christina M. , Jackson Jordan E. , Rajasekar Ganesh , Nuño Miriam , Yamashiro Kaeli J. , Farmer Diana L. , Hirose Shinjiro , Brown Erin G. 
Published: January 2021

Abstract
Purpose
Prescription drug monitoring programs (PDMPs) have been established to combat the opioid epidemic, but there is no data on their efficacy in children. We hypothesized that a statewide PDMP mandate would be associated with fewer opioid prescriptions in pediatric surgical patients.

Methods
Patients < 18 undergoing inguinal hernia repair, orchiopexy, orchiectomy, appendectomy, or cholecystectomy at a tertiary children’s hospital were included. The primary outcome, discharge opioid prescription, was compared for 10 months pre-PDMP (n = 158) to 10 months post-PDMP (n = 228). Interrupted time series analysis was performed to determine the effect of the PDMP on opioid prescribing.

Results
Over the 20-month study period, there was an overall decrease in the rate of opioid prescriptions per month (− 3.6% change, p < 0.001). On interrupted time series analysis, PDMP implementation was not associated with a significant decrease in the monthly rate of opioid prescriptions (1.27% change post-PDMP, p = 0.4). However, PDMP implementation was associated with a reduction in opioid prescriptions of greater than 5 days’ supply (− 2.7% per month, p = 0.03).

Conclusion
Opioid prescriptions declined in pediatric surgical patients over the study time period. State-wide PDMP implementation was associated with a reduction in postoperative opioid prescriptions of more than 5 days’ duration.
Auditory performance in a group of elderly patients after cochlear implantation
Authors: Giourgas Alexandros , Durisin Martin , Lesinski-Schiedat Anke , Illg Angelika , Lenarz Thomas 
Published: January 2021

AbstractPurposeThe retrospective case review investigated the effect of cochlear implantation in subjects aged 61 years or older with respect to their auditory performance. The study also analysed the effect of age on the performance, and it drew a comparison between the outcomes of older and younger adults.MethodsThe outcome in a group of 446 patients aged 61 to 89 years at the time of unilateral cochlear implantation was compared with the outcome in a group of 110 patients aged 17 to 42 years. Auditory performance was measured with open-set monosyllabic word testing and sentences in quiet and in noise.ResultsIn the monosyllabic word recognition test, the group of older adults performed significantly better after cochlear implantation compared with their scores prior to implantation (p < 0.001;r = 0.59). Their auditory performance correlated negatively with their age. However, the correlation was of small strength. Significant differences in auditory performance were detected between sexagenarians and octogenarians (p < 0.001;r = 0.27). Additionally, a statistically significant difference was revealed between the groups of older and younger adults in the monosyllabic word test (p = 0.001;r = 0.15).ConclusionElderly cochlear implant recipients can benefit significantly from cochlear implantation. Although higher age correlates negatively with auditory performance, its influence in the presented sample is small.
Efficacy and safety of interleukin-17A inhibitors in patients with ankylosing spondylitis: a systematic review and meta-analysis of randomized controlled trials
Authors: Wang Peng , Zhang Shuo , Hu Binwu , Liu Weijian , Lv Xiao , Chen Songfeng , Shao Zengwu 
Published: January 2021

AbstractTo assess the efficacy and safety of interleukin (IL)-17A inhibitors in patients with ankylosing spondylitis (AS). PubMed, EMBASE, and Web of Science were searched up to 5 February 2020 for randomized controlled trials (RCTs) that assessed the efficacy and safety of IL-17A inhibitors in patients with AS. We used a meta-analytic approach to perform a random effects analysis or fixed effects analysis according to heterogeneity. Subgroup analyses between studies included medication, time to primary endpoint, and data source. Odds ratios (ORs) or mean differences (MDs) were used to assess the efficacy and safety of IL-17A inhibitors in AS. A total of ten RCTs with 2613 patients were eligible for inclusion in the analysis (six for secukinumab, two for ixekizumab, one for netakimab, and one for bimekizumab). Compared to placebo, IL-17A inhibitors improved ASAS20 response rate (OR = 2.58;p < 0.01) and ASAS40 response rate (OR = 2.80;p < 0.01), and significantly increased the risk of AEs (OR = 1.23;p = 0.03) and nasopharyngitis (OR = 1.72;p < 0.01), but not SAEs (OR = 0.87;p = 0.60). IL-17A inhibitors demonstrated better efficacy in patients with AS in several evaluation indicators. However, the safety of IL-17A inhibitors remains to be further studied in studies with larger sample size and longer follow-up times.
Porter Hypothesis vs Pollution Haven Hypothesis: Can There Be Environmental Policies Getting Two Eggs in One Basket?
Authors: Ranocchia Claudia , Lambertini Luca 
Published: January 2021

AbstractThe Porter hypothesis and the pollution haven hypothesis seem to predict opposite reactions by firms facing environmental regulation, as the first invokes the arising of a win–win solution while the second envisages the possibility for firms to flee abroad.
We illustrate the possibility of designing policies (taking the form of either emission taxation or environmental standards) able to eliminate firms’ incentives to relocate their plants abroad and create a parallel incentive for them to deliver a win–win solution by investing either in replacement technologies under emission taxation, or in abatement technologies under an environmental standard. This is worked out in a Cournot supergame in which firms may activate the highest level of collusion compatible with their intertemporal preferences.
Pesticide residues in daily bee pollen samples (April–July) from an intensive agricultural region in Southern Germany
Authors: Friedle Carolin , Wallner Klaus , Rosenkranz Peter , Martens Dieter , Vetter Walter 
Published: January 2021

AbstractInsect-pollinated plants are essential for honey bees to feed their brood. In agricultural landscapes, honey bees and other pollinators are often exposed to pesticides used for cultivation. In order to gain more insight into the fluctuation of pesticide loads, 102 daily pollen samples were collected between April and July 2018 in a fruit-growing area in Southern Germany. Samples were analyzed with respect to more than 260 pesticides using a multi-residue pesticide analysis method. Almost 90% of the analyzed pollen samples featured between one and thirteen different pesticides. In total, 29 pesticides were detected at maximum concentrations of up to 4500 ng/g pollen. Maximum residual concentrations of most pesticides were observed during April and the first half of May, as well as during the second half of June. In most cases, serial data of pesticide residuals were detected for approximately 10 subsequent days with two or three maximum values, which were several folds higher than concentrations on the days before and thereafter. The pollen hazard quotient (PHQ) was calculated to estimate the risk of the detected pesticides to honey bees and wild pollinators.
Understanding teacher design practices for digital inquiry–based science learning: the case of Go-Lab
Authors: de Jong Ton , Gillet Denis , Rodríguez-Triana María Jesús , Hovardas Tasos , Dikke Diana , Doran Rosa , Dziabenko Olga , Koslowsky Jens , Korventausta Miikka , Law Effie , Pedaste Margus , Tasiopoulou Evita , Vidal Gérard , Zacharia Zacharias C. 
Published: January 2021

AbstractDesigning and implementing online or digital learning material is a demanding task for teachers. This is even more the case when this material is used for more engaged forms of learning, such as inquiry learning. In this article, we give an informed account of Go-Lab, an ecosystem that supports teachers in creating Inquiry Learning Spaces (ILSs). These ILSs are built around STEM–related online laboratories. Within the Go-Lab ecosystem, teachers can combine these online laboratories with multimedia material and learning apps, which are small applications that support learners in their inquiry learning process. The Go-Lab ecosystem offers teachers ready–made structures, such as a standard inquiry cycle, alternative scenarios or complete ILSs that can be used as they are, but it also allows teachers to configure these structures to create personalized ILSs. For this article, we analyzed data on the design process and structure of 2414 ILSs that were (co)created by teachers and that our usage data suggest have been used in classrooms. Our data show that teachers prefer to start their design from empty templates instead of more domain–related elements, that the makeup of the design team (a single teacher, a group of collaborating teachers, or a mix of teachers and project members) influences key design process characteristics such as time spent designing the ILS and number of actions involved, that the characteristics of the resulting ILSs also depend on the type of design team and that ILSs that are openly shared (i.e., published in a public repository) have different characteristics than those that are kept private.
A Matter of Timing—Pregnancy After Bariatric Surgery
Authors: Heusschen Laura , Krabbendam Ineke , van der Velde Jessika M. , Deden Laura N. , Aarts Edo O. , Merién Ashley E. R. , Emous Marloes , Bleumink Gysèle S. , Lutgers Helen L. , Hazebroek Eric J. 
Published: January 2021

Abstract
Purpose
Current guidelines recommend to avoid pregnancy for 12–24 months after bariatric surgery because of active weight loss and an increased risk of nutritional deficiencies. However, high-quality evidence is lacking, and only a few studies included data on gestational weight gain. We therefore evaluated pregnancy and neonatal outcomes by both surgery-to-conception interval and gestational weight gain.

Materials and Methods
A multicenter retrospective analysis of 196 singleton pregnancies following Roux-en-Y gastric bypass, sleeve gastrectomy, and one anastomosis gastric bypass was conducted. Pregnancies were divided into the early group (≤ 12 months), the middle group (12–24 months), and the late group (> 24 months) according to the surgery-to-conception interval. Gestational weight gain was classified as inadequate, adequate, or excessive according to the National Academy of Medicine recommendations.

Results
Pregnancy in the early group (23.5%) was associated with lower gestational age at delivery (267.1 ± 19.9 days vs 272.7 ± 9.2 and 273.1 ± 13.5 days, P = 0.029), lower gestational weight gain (− 0.9 ± 11.0 kg vs + 10.2 ± 5.6 and + 10.0 ± 6.4 kg, P < 0.001), and lower neonatal birth weight (2979 ± 470 g vs 3161 ± 481 and 3211 ± 465 g, P = 0.008) than pregnancy in the middle and late group. Inadequate gestational weight gain (40.6%) was associated with lower gestational age at delivery (266.5 ± 20.2 days vs 273.8 ± 8.4 days, P = 0.002) and lower neonatal birth weight (3061 ± 511 g vs 3217 ± 479 g, P = 0.053) compared to adequate weight gain. Preterm births were also more frequently observed in this group (15.9% vs 6.0%, P = 0.037).

Conclusion
Our findings support the recommendation to avoid pregnancy for 12 months after bariatric surgery. Specific attention is needed on achieving adequate gestational weight gain.
Primary and Secondary/ Metastatic Salivary Duct Carcinoma Presenting within the Sinonasal Tract
Authors: Agaimy Abbas , Mueller Sarina K. , Bishop Justin A. , Chiosea Simion I. 
Published: January 2021

AbstractTraditionally, sinonasal adenocarcinomas have been subdivided into intestinal (ITAC) and non-intestinal (non-ITAC) categories. The latter encompasses salivary-type adenocarcinomas originating from the seromucinous glands of the sinonasal mucosa and non-salivary adenocarcinomas. The non-salivary adenocarcinoma category is further subdivided into low-and high-grade variants. Among salivary-type sinonasal adenocarcinomas, tumors recapitulating salivary duct carcinoma (SDC) are exceedingly rare, but some might have been lumped into the high-grade non-ITAC category. To date, only three primary SDCs originating in the sinonasal tract have been reported. We herein describe 7 cases of SDC including one previously reported case (4 primary sinonasal, 3 metastatic/ extension from parotid gland SDC). The primary tumors affected 3 males and one female aged 60 – 75. Different sites were involved by the primary tumors while the secondary tumors affected the sphenoidal (2) and the frontal + maxillary (1) sinuses. Three primary tumors were de novo high-grade SDC and one was confined to contours of a pre-existing pleomorphic adenoma. All 3 secondary tumors were SDC ex pleomorphic adenoma of the parotid with a long history of recurrences, ultimately involving the sinonasal tract. Androgen receptor was positive in 7/7 cases. Four of 6 cases were strongly HER2/neu + (either score 3 + or with verified amplification). This small case series adds to the delineation of primary sinonasal SDC highlighting that almost half of invasive SDC presenting within sinonasal tract indeed represents extension or metastasis from a parotid gland primary. There is a tendency towards overrepresentation of HER2/neu-positive cases in both categories (primary and metastatic), but this needs clarification in larger studies.