bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Research on Material Removal Mechanism and Grinding Force of FeCoNiCrMo0.1 High Entropy Alloy (HEA)
Authors: Xu Ruchu , Wen Xuelong , Gong Yadong , Yu Xingchen 
Published: September 2021

Abstract
High entropy alloy (HEA) is an advanced alloy material, which has a wide application prospect due to its excellent properties. However, the material removal mechanism and change rule of grinding force of HEA in the grinding process have seldom been studied. The main work of this paper is that the material removal mechanism of the FeCoNiCrMo0.1 HEA is obtained by analyzing grinding debris and subsurface microstructure after grinding, the theoretical grinding force model of HEAs in plane grinding process is established on the basis of the force of a single abrasive grain, and the experimental verification is performed. According to the experimental results, the influences of different grinding parameters on grinding force are discussed, the influences of different types of grinding wheels on grinding force are analyzed, and the grinding forces generated by grinding different FeCoNiCr HEAs are compared. The results indicate that the material removal mechanism of FeCoNiCrMo0.1 HEA is the plastic removal. With the increase of grinding speed and the decrease of grinding depth and feed speed, both normal and tangential grinding forces decrease. Under the same grinding parameters, the grinding force produced by electroplated CBN grinding wheel is greater, followed by resin-bonded CBN grinding wheel and vitrified CBN grinding wheel. The grinding force produced by grinding FeCoNiCrAl0.1 HEA is lower than that produced by grinding FeCoNiCrMo0.1 HEA under the same grinding conditions. The calculated value of grinding force model is consistent with the experimental value, which can scientifically reflect the variation law of HEA grinding force.
Risk of Metastatic Disease Using [18F]PSMA-1007 PET/CT for Primary Prostate Cancer Staging
Authors: Chikatamarla Venkata Avinash , Okano Satomi , Jenvey Peter , Ansaldo Alexander , Roberts Matthew J. , Ramsay Stuart C. , Thomas Paul A. , Pattison David A. 
Published: September 2021

Abstract
Purpose: Accurate prostate cancer imaging is critical for patient management. Multiple studies have demonstrated superior diagnostic accuracy of [68Ga]-PSMA-11 PET/CT over conventional imaging for disease detection, with validated clinical and biochemical predictors of disease detection. More recently [18F]PSMA-1007 offers theoretical imaging advantages, but there is limited evidence of clinical and biochemical predictors of scan findings in the staging population. This study investigates the correlation of clinical variables with imaging characteristics among patients who underwent [18F]PSMA-1007 PET/CT for primary staging of men with histopathologically confirmed prostate carcinoma. Methods: A retrospective review of 194 consecutive patients imaged between May 2019 to May 2020 was performed. Imaging performance (presence and distribution of metastatic disease, primary tumour avidity) was assessed considering clinical variables (EAU risk criteria) with logistic regression and ROC curve analysis. Results: ISUP grade was associated with the presence of metastasis overall (p=0.008) as well as regional nodal (p = 0.003), non-regional nodal (p = 0.041) and bone (p = 0.006) metastases. PSA level was associated with metastatic disease overall (p=0.001), regional (p = 0.001) and non-regional nodal metastases (p = 0.004), but not with bone metastases (p = 0.087). There were too few visceral metastases for meaningful analysis. SUVmax of the primary prostatic tumour was associated with ISUP grade (p = 0.004), PSA level (p < 0.001) and AJCC stage (p = 0.034). PSA > 20 ng/mL and ISUP grade > 3 had a specificity of 85% (95% CI 78-91%) and 60% (95% CI 50-68%), and a sensitivity of 36% (95% CI 25-49%) and 62% (95% CI 49-74%) respectively, for detection of metastatic disease.Conclusion: Metastatic disease according to [18F]PSMA-1007 PET/CT was associated with ISUP grade and PSA level. This is the largest study using [18F]PSMA-1007 PET/CT to confirm a positive correlation of PSA level, ISUP grade and stage with primary prostate tumour SUVmax.
The Absorption Characteristics and the Genetic Response of Beauveria Bassiana Z1 under Cd2+ Stress
Authors: Yu Tiantian , Zhang Lijie , Gao Yanhui , Ma Zhengfa , Zhang Mei , Tan Weiqun , Zhang Lei , Zhang Yunru , Zhao Tiantao 
Published: September 2021

Abstract
Cadmium (Cd2+) has carcinogenic and teratogenic toxicity, which can be accumulated in the human body through the food chain, endangering human health and life. In this study, a fungus named Beauveria bassiana Z1 was isolated and identified to absorb Cd2+, and its Cd2+ absorption rate was 85.1 % when the Cd2+ concentration was 1 mM. Furthermore, the absorption rate of activated strain was significantly higher than that of inactivated, especially with a high Cd2+ concentration. The genetic response results show that the expression of Reactive Oxygen Species (ROS) scavenging enzyme gene and the stress resistance genes (p-type ATP transferase, heavy metal tolerance protein, cytochrome P450) was up-regulated, which was conducive to the ROS removal and heavy metal modification, thereby improving the tolerance of strain Z1 to Cd2+. The research results are of great significance for elucidating the mechanism of fungi for heavy metal, and provide theoretical support for bioremediation of heavy metal pollution.
Oral GLP-1 Analogue Ameliorates Obesity-Induced Diabetes In Db/Db Mouse
Authors: Zhang Hanlin , Dong Meng , Jin Wanzhu 
Published: September 2021

Abstract
Type 2 diabetes is currently experiencing an outbreak worldwide. GLP-1 effectively lowers blood glucose level as an emerging target for the treatment of type 2 diabetes. However, the application of GLP-1 is limited by short half-life and too expensive cost in clinic. In this study we employed the food-grade probiotics as delivery system to express human GLP-1 and its analogue. Recombinant lactococcus lactis could express GLP-1 and analogue in vitro and modified GLP-1 analogue was more resistant to DPP-4 degradation. Oral administration of GLP-1 analogue could reduce the fat mass. More importantly, GLP-1 analogue improved hyperglycemia and insulin resistance in Db/Db mouse although the insulin secretion is not observed in vitro. Our study demonstrates that lactococcus lactis genetically modified with single amino acid mutation could prolong half-life of GLP-1 and increase insulin sensitivity in Db/Db mouse model as an oral drug delivery system driving the development and innovation of drug therapy for type 2 diabetes.
Fuel Gas Production From Self-Sustaining Smouldering Combustion of Lignocellulosic Waste
Authors: Wyn Hons K , Konarova Muxina , Perkins Greg , Yermán Luis 
Published: September 2021

Abstract
Smouldering combustion has shown to be an effective application for soil remediation and as a waste treatment method for solids with high moisture content. The experimental set-up of smouldering combustion reactors is similar to autothermal fixed-bed gasification, updraft reactor configuration. In this study, smouldering experiments were conducted using lignocellulosic agricultural waste. The moisture content of lignocellulosic biomass was varied between 10 % to 50 %. Air flux was varied between 1.8 and 7.4 cm/s. Experiments were also conducted with varying oxygen concentration in the airflow (3.6 – 21 %), addition of sand (4 and 8 g/g) and other lignocellulosic material (wood pellets, and residual berry plant). Fuel gas with maximum H2, CO, CH4, CO2 concentrations of 7.7, 32.6, 2.3, and 57.4 % (N2 free) respectively were obtained with 10 % moisture content and 7.4 cm/s air flux. The smouldering yielded 1.24 Nm3/kgfeed_dry of gas with calorific value of 1.82 MJ/Nm3 (HHV).
The Senescence Difference Between the Central and Peripheral Cornea Induced by Sutures
Authors: Li Suxia , Dong Qiaoqiao , Dong Muchen , Qu Mingli , Wang Yao , zhou Qingjun , Shi Weiyun 
Published: September 2021

Abstract
Introduction: Cell senescence plays a regulatory role in tissue fibrosis, and corneal scarring is usually more severe in the central cornea based on clinical observation. We attempted to explore the senescence difference between the central and peripheral cornea in an in vivo mouse model of suture-induced senescence and an in vitro model of senescence with hydrogen peroxide (H2O2)-induced rabbit corneal fibroblasts. Methods: Male Balb/c mice (6-8 weeks) received sutures in the central, superior, inferior, nasal, and temporal cornea, and the sutures were removed on day 14. Corneal neovascularization (CNV) was observed under a slit lamp microscope with a digital camera. Fibroblasts isolated from central and peripheral rabbit corneas were induced with H2O2 to establish an in vitro model of senescence. The senescence was evaluated with SA-β-gal staining and gene expression analysis of p21, p27, and p53. Results: Senescent cells accumulated in the corneal stroma from day 3 to day 14 after the operation and peaked on day 14, later than CNV. More senescent keratocytes were observed in the peripheral cornea in the mouse model. The peripheral corneal fibroblasts were more likely to become senescent related to H2O2 in vitro. The PCR results revealed that the senescence-related genes, p21, p27, and p53, were all highly expressed in the peripheral corneal fibroblasts. Conclusions: As senescent fibroblasts can limit tissue fibrosis, the senescence difference between the central and peripheral cornea may contribute to the difference in scarring.
The Transcriptome-Wide N6-Methyladenosine (m6A) Map Profiling Reveals the Regulatory Role of m6A in the Yak Ovary
Authors: Guo Shaoke , Wang Xingdong , Cao Mengli , Wu Xiaoyun , Xiong Lin , Bao Pengjia , Chu Min , Liang Chunnian , Yan Ping , Pei Jie , Guo Xian 
Published: September 2021

Abstract
Background and aim: Yak estrus is a seasonal phenomenon, probably involving epigenetic regulation of synthesis and secretion of sex hormones as well as growth and development of follicles. N6-methyladenosine (m6A) is the most common internal modification of the eukaryotic mRNA. However, there are no detailed reports on the m6A transcriptome map of yak ovary. Therefore, this study aimed to collected the yak ovarian tissues at three different states of anestrus (YO-A), estrus (YO-F), and pregnancy (YO-P), and obtained the full transcriptome m6A map in yak by MeRIP-seq. Results: The HE staining revealed that the number of growing follicles and mature follicles in the ovary during the estrus period was relatively higher than those in the estrus period and the pregnancy period. The RT-qPCR showed that the expression of METTL3, METTL14, FTO, YTHDC1 were significantly different across different periods in the ovaries, which suggests that m6A may play a regulatory role in ovarian activity. Next, we identified 20174, 19747 and 13523 m6A peaks in the three ovarian samples of YO-A, YO-F and YO-P using the methylated RNA immunoprecipitation sequencing (MeRIP-seq). The m6A peaks are highly enriched in the coding sequence (CDS) region and 3′untranslated region (3′UTR) as well as the conserved sequence of “RRACH.” Functional analysis revealed the involvement of m6A in many physiological activities of the yak’s ovary during reproductive cycle. The association analysis found that some genes such as BNC1, HOMER1, BMP15, BMP6, GPX3, and WNT11 were related to ovarian functions. Conclusions: The comparison of the distribution patterns of methylation peaks in the ovarian tissues across different periods further explored the m6A markers related to the regulation of ovarian ovulation and follicular development in the yak ovary. This comprehensive map provides a solid foundation for revealing the potential function of the mRNA m6A modification in the yak ovary.
Rifaximin-Mediated Gut Microbiota Regulation Modulates the Polarization of Microglia During CUMS in Rat
Authors: Li Haonan , Xiang Yujiao , Zhu Zemeng , Wang Wei , Jiang Zhijun , Zhao Mingyue , Cheng Shuyue , Pan Fang , Liu Dexiang , Ho Roger , Ho Cyrus 
Published: September 2021

Abstract
Background: Chronic unpredictable mild stress (CUMS) can not only lead to depression-like behavior but also change the composition of the gut microbiome. Regulating the gut microbiome can have an antidepressant effect, but the mechanism by which it improves depressive symptoms is not clear. Short-chain fatty acids (SCFAs) are small molecular compounds produced by the fermentation of non-digestible carbohydrates. SFCAs are ubiquitous in intestinal endocrine and immune cells, making them important mediators of gut microbiome-regulated body functions. Activated M1 microglia can cause pro-inflammatory and neurotoxic effects, while M2 microglia serve anti-inflammatory and neuroprotective functions. The balance between the two phenotypes of microglia plays an important role in the occurrence and treatment of depression caused by chronic stress. We hypothesized that rifaximin exerts an antidepressant effect by changing the abundance of fecal SFCA metabolites and transforming the microglial phenotype. Methods: We administered 150 mg/kg rifaximin intragastrically to rats exposed to CUMS for 4 weeks and investigated the composition of the fecal microbiome, the content of short-chain fatty acids in the serum and brain, microglial phenotypic profiles and hippocampal neurogenesis. Results: Our results show that rifaximin ameliorated depressive-like behavior induced by CUMS, as reflected by sucrose preference, the open field test and the Morris water maze. Rifaximin increased the relative abundance of Ruminococcaceae, which were significantly positively correlated with high levels of butyrate in the brain. Rifaximin also increased the transformation of M1 microglia into the M2 type in the hippocampal dentate gyrus (DG) and ameliorated neurogenic abnormalities and functional deficits caused by CUMS.Conclusions: These results suggest that rifaximin can enhance the neuroprotective effect of microglia to some extent by regulating the gut microbiome and one of its metabolites, butyrate.
The Impact of Atmospheric Rossby Waves and Cyclones On The Arctic Sea Ice Variability
Authors: Hofsteenge Marte Gé , Graversen Rune Grand , Rydsaa Johanne Hope , Rey Zoé 
Published: September 2021

Abstract
The Arctic sea-ice extent has strongly declined over recent decades. A large inter-annual variability is superimposed on this negative trend. Previous studies have emphasised a significant warming effect associated with latent energy transport into the Arctic region, in particular due to an enhanced greenhouse effect associated with the convergence of the humidity transport over the Arctic. The atmospheric energy transport into the Arctic is mostly accomplished by waves such as Rossby waves and cyclones. Here we present a systematic study of the effect on Arctic sea ice of these atmospheric wave types. Through a regression analysis we investigate the coupling between transport anomalies of both latent and dry-static energy and sea-ice anomalies. From the state-of-the-art ERA5 reanalysis product the latent and dry-static transport over the Arctic boundary (70 ºN) is calculated. The transport is then split into transport by planetary and synoptic-scale waves using a Fourier decomposition. The results show that latent energy transport as compared to that of dry-static shows a much stronger potential to decrease sea ice concentration. However, taking into account that the variability of dry-static transport is of an order of magnitude larger than latent, the actual impact on the sea ice appears similar for the two components. In addition, the energy transport by planetary waves causes a strong decline of the sea ice concentration whereas the transport by synoptic-scale waves shows only little effect on the sea ice. The study emphasises the importance of the large-scale waves on the sea ice variability.
Electrochemical Degradation of Reactive Dyes in Textile Industrial Wastewater by Modified Electrodes
Authors: Gezahegn Getasew Yehuala 
Published: September 2021

Abstract
This research was formulated to introduce an electrochemical degradation for the degradation of reactive dyes of the textile industrial wastewater applying a full factorial experimental design. Electrochemical degradation is a green process which immensely reduces the chemical consumption of the wastewater leaving no impurities. Three factors with three-levels were used i.e. the NaCl electrolyte concentrations of 2, 4.5, 5.8 g/L, pH 4.2, 5.8, and 6.9, and reaction time 12.4, 15.5, and 20 min. The experiment was expected to generate 27 runs but due to the Design Expert 12, the number of experimental runs became 20. DC power supply of 14.6 V was used for the electrochemical degradation system whereas chemicals such as nitric acid (400 g/L), sulphuric acid (1.5 mg/L), and oxalic acid solution (100 g/L) were used for surface modification of the electrodes. The textile industrial wastewater was characterized based on the standard method of APHA. Dye, COD, BOD5, and pH of the textile industrial effluent were found to be 0.35 ± 1.00 Abs., 1,711.55 ± 2.00 mg/L, 450.00 ± 3.00 mg/L, and pH of 6.63 ± 0.30, respectively. The maximum color removal was 94.89% at the optimum experimental condition after the results validated by design expert 12. These optimum conditions were the electrolyte concentration 5.1 g/L, pH 6.5, and reaction time 17 min, whereas the minimum color removal of 63.0% was recorded. The regression analysis of the color removal (R2 = 0.95) depicts that electrolyte concentration was the dominant factor for the electrochemical degradation.
Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells
Authors: Shah Kathleen , Maradana Muralidhara Rao , Delas M Joaquina , Metidji Amina , Graelmann Frederike , Llorian Miriam , Chakravarty Probir , Li Ying , Tolaini Mauro , Shapiro Michael , Kelly Gavin , Stockinger Brigitta 
Published: September 2021

Abstract
The aryl hydrocarbon receptor (AHR) is an environmental sensor that integrates microbial and dietary cues to influence physiological processes within the intestinal microenvironment, protecting against colitis and colitis-associated colorectal cancer development. Rapid tissue regeneration upon injury is important for the reinstatement of barrier integrity and its dysregulation promotes malignant transformation. Here we show that AHR is important for the termination of the regenerative response and the reacquisition of mature epithelial cell identity post injury in vivo and in organoid cultures in vitro. Using an integrative multi-omics approach in colon organoids, we show that AHR is required for timely termination of the regenerative response through direct regulation of transcription factors involved in epithelial cell differentiation as well as restriction of chromatin accessibility to regeneration-associated Yap/Tead transcriptional targets. Safeguarding a regulated regenerative response places AHR at a pivotal position in the delicate balance between controlled regeneration and malignant transformation.*As a note, Kathleen Shah and Muralidhara Rao Maradana are joint first authors.
Multistakeholder Perspectives on the Mistreatment of Indigenous Women During Childbirth in Colombia: Drivers and Points for Intervention
Authors: Gleason Emily Gaffney , Ríos Jennifer Marcela López , Berrío Diana Patricia Molina , Merino Cristina Mejía 
Published: September 2021

Abstract
Background: Abusive and disrespectful treatment of women during childbirth is a critical global issue that threatens women’s sexual rights and reproductive rights and access to quality maternal care. This phenomenon has been documented in Colombia. However, little emphasis has been placed on identifying the drivers of and potential interventions against disrespect and abuse against particularly vulnerable populations in the country, including internally displaced indigenous women. Methods: This report is a sub-analysis of a larger project. Semi-structured interviews were conducted with indigenous (Embera) women with childbirth experience (n=10), maternal healthcare workers (n=6), and community stakeholders (n=5) in Medellín, Colombia. Qualitative analysis techniques, consisting of inductive and deductive approaches, were used to identify and characterize the drivers of disrespect and abuse against indigenous women during childbirth and points for intervention. Existing frameworks were adapted to thematically organize drivers and potential solutions into four interrelated subsystems: individual and community factors, clinician factors, facility factors, and national health system factors.Results: Participants highlighted risk disrespect and abuse as stemming from (within the individual and community level) its normalization, lack of autonomy and empowerment among indigenous women, lacking antenatal care, (within the clinician level) prejudice, linguistic or cultural barriers to communication, lack of understanding of indigenous culture, medical culture and training, burnout and demoralization, (within the facility level) inadequate infrastructure, space, and human resources, and (within the national systems level) lack of clear policies and the devaluing of respectful maternity care. They called for interventions specific to these drivers, grounded in dignity and respect for indigenous culture.Conclusion: This paper expands upon the growing literature on global mistreatment during childbirth by highlighting drivers of mistreatment and identifying points for intervention in a previously unstudied population. Our data show that indigenous women are especially vulnerable to mistreatment due to cultural and linguistic barriers and prejudice. Broad and meaningful action is urgently needed to realize these women’s rights to respectful maternity care. Interventions must be multifaceted and locally specific, taking into account the needs and wants of the women they serve.
Verification of a Cryptic T(Y;15) Translocation in a Male With an Apparent 45,X Karyotype
Authors: Qin Shengfang , Zhang Zhuo , Chen Ximin , Yin Yan , Ye Mengling , Li-Ling Jesse 
Published: September 2021

Abstract
BackgroundA rare disease is that an individual with a non-chimeric karyotype of 45, X develops into a male. We explored the genetic aetiology of an infertility male with an apparent 45, X karyotype, which was subsequently verified as cryptic translocation between chromosomes Y and 15. MethodsPeripheral blood sample was collected from the patient and subjected to a range of genetic testing, including conventional chromosomal karyotyping, short tandem repeat (STR) analysis for azoospermia factor (AZF) region, fluorescence in situ hybridization (FISH) with specific probes for CSP X/CSP Y, CSP Y/D15Z1/PML and SRY/D15Z1/PML, and chromosomal microarray analysis (CMA) for genomic copy number variations (CNVs). ResultsThe patient was found to have an apparent 45,X karyotype. STR analysis showed that he possessed a short arm of the Y chromosome, including the SRY gene but the absence of a long arm of the Y chromosome, including AZFa+b+c and Yqter. A FISH assay using CSP X and CSP Y probes showed a green signal at the centromere of the X chromosome and a red signal for the Y centromeric sequence on a D-group-sized chromosome. By FISH assaying with D15Z1 and CSP Y probes, chromosomes 15 and Y centromeric signals appeared closely on a single chromosome, as ascertained by the PML control probe. A further FISH assay with D15Z1 and SRY probes revealed a signal of the SRY gene at the end of one arm of chromosome 15. The result of the CMA indicated a deletion with an approximate size of 45.31 Mb spanning from Yq11 to Yter. Conclusion﻿Although the 45,X male patient did not harbour an intact Y chromosome, his genome contained the SRY gene derived from the translocation of the Yp, which probably triggered the male differentiation and development. Imbalanced translocations of Yp to other chromosomes can result in short stature and infertility among patients. Delineation of the genetic aetiology can guide early intervention and assisted reproduction in adulthood.