bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vivo‐Like Scaffold‐Free 3D In Vitro Models of Muscular Dystrophies: The Case for Anchored Cell Sheet Engineering in Personalized Medicine
Authors: Shahin‐Shamsabadi Alireza , Cappuccitti John 
Published: December 2024

AbstractProgress in understanding the underlying mechanisms of muscular dystrophies is hindered by the lack of pathophysiologically relevant in vitro models. Here, an entirely scaffold‐free anchored cell sheet engineering platform is used to create patient‐specific three‐dimensional (3D) skeletal muscle in vitro models. This approach effectively replicates mature muscle phenotypes and tissue‐ and disease‐specific extracellular matric (ECM). Models were developed using primary cells from healthy individuals and patients with Duchenne Muscular Dystrophy and Myotonic Dystrophy Type 1. Through a combination of quantified histological staining (Hematoxylin & Eosin, Movat's Pentachrome, Masson's Trichrome) and immunostaining (desmin, myosin heavy chain, laminin, and dystrophin), it was demonstrated that the models formed mature constructs closely resembling their respective in vivo conditions. Proteomics analysis revealed that the models exhibited appropriate upregulation and downregulation of disease‐relevant pathways. Models of diseased tissues accurately reflected key phenotypic features of the diseases, including alterations in muscle fiber integrity and ECM composition. Upon treatment with therapeutically beneficial drugs, significant changes in their proteomic profiles were documented, highlighting the models’ potential for drug screening. This novel in vitro modeling approach, unlike other 3D techniques that rely on exogenous biomaterials that interfere with natural cellular behaviors, provides a promising platform for studying muscular dystrophies.
A Versatile and High‐Resolution Hydrogel Platform for Volumetric Additive Manufacturing Based on Poly(ethylene glycol) Diacrylate and Alginate Blends
Authors: Hall Patrick C. , Chasko DeShea , Wheeler Tim , Ostojich Diana , Aptecker Judah , Liashenko Ievgenii , Luxenhofer Robert , Lindberg Gabriella C.J. , Dalton Paul D. 
Published: December 2024

AbstractVolumetric additive manufacturing (VAM) has emerged as a potent method for fabricating complex structures out of soft materials such as hydrogels. In this study, a low‐cost photopolymer platform is developed based on low molecular weight poly (ethylene glycol) diacrylate (PEGDA) and alginate (Alg) blends which reduces material costs ≈1000x. By adding non‐crosslinked 0.5 wt.% of Alg to these 5, 10, or 15 wt.% PEGDA precursor solutions, the viscosity is raised from <1 mPa*s to 50 mPa*s, which enables VAM of low molecular weight PEGDA. The fabricated hydrogels have customizable mechanical properties, ranging from 14 ± 4 kPa to 90 ± 24 kPa, and 289 ±121 kPa and correspond to equilibrium water contents of 96.8 ± 0.3%, 91.2% ± 0.8% and 84.1% ± 0.9%. The printed structures have minimum feature sizes ranging between 56 ± 9 µm for 5 wt.% PEGDA + 0.5 wt.% Alg, 47 ± 12 µm for 10 wt.% PEGDA + 0.5 wt.% Alg, and 39 ± 7 µm for 15 wt.% PEGDA + 0.5 wt.% Alg. Additionally, these materials are printable into designs with internal voids, unsupported struts, and interlocked features. This work establishes a low cost, mechanically tunable hydrogel platform for VAM which can improve accessibility and adoption.
MLLT3 Regulates Melanoma Stemness and Progression by Inhibiting HMGB1 Nuclear Entry and MAGEA1 M5C Modification
Authors: Li Yaling , Liu Hong , Li Jingyi , Fu Chang , Jiang Bin , Chen Bancheng , Zou Yanfen , Yu Bo , Song Bing 
Published: December 2024

AbstractMelanoma stem cells are a kind of cells with self‐renewal and multi‐directional differentiation potential. They are one of the key factors in the occurrence, development and metastasis of melanoma. This study demonstrates that MLLT3 is a transcription factor that regulates the stemness and progression of melanoma. MLLT3 interacted with HMGB1 to inhibit its entry into the nucleus, MLLT3 interacted with YBX1 to inhibit its reading of m5C of MAGEA1, thereby inhibiting the mRNA stability of MAGEA1, and directly transcribed P53 to inhibit the stemness, proliferation and metastasis of melanoma cells. This study further explored the potential mechanism of the interaction between miR‐542‐3p/miR‐3922‐3p and MLLT3. Furthermore, the scRNA‐seq of melanoma cells with MLLT3 knock‐out resulted in important changes in cell subsets, activating the TP53 and MAPK pathways and transforming into stem cells. The results indicate that the transcription factor MLLT3 is a suppressor gene that regulates the stemness and progression of melanoma, and is expected to become a target for melanoma therapy.
Tunable Picoliter‐Scale Dropicle Formation Using Amphiphilic Microparticles with Patterned Hydrophilic Patches
Authors: Song Xinpei , Udani Shreya , Ouyang Mengxing , Sahin Mehmet Akif , Di Carlo Dino , Destgeer Ghulam 
Published: December 2024

AbstractMicroparticle‐templated droplets or dropicles have recently gained interest in the fields of diagnostic immunoassays, single‐cell analysis, and digital molecular biology. Amphiphilic particles have been shown to spontaneously capture aqueous droplets within their cavities upon mixing with an immiscible oil phase, where each particle templates a single droplet. Here, an amphiphilic microparticle with four discrete hydrophilic patches embedded at the inner corners of a square‐shaped hydrophobic outer ring of the particle (4C particle) is fabricated. Three dimensional computational fluid dynamics simulations predict droplet formation dynamics and differing equilibrium conditions depending on the patterning configuration. Experiments recapitulate equilibrium conditions, enabling tunable dropicle configurations with reproducible volumes down to ≈200 pL templated by the amphiphilic particles. The dropicle configurations depend predominantly on the size of the hydrophilic patches of the 4C particles. This validates that the modeling approach can inform the design of dropicles with varying volumes and numbers per particle, which can be harnessed in new amplified bioassays for greater sensitivity, dynamic range, and statistical confidence.
Extraction of valuable metals from waste Li‐ion batteries by deep eutectic solvent: Experimental and mechanism analysis
Authors: Zhang Yan , Liu Hai , Hu Ruoyu , Zhou Yan , Wang Shuai , Qi Jianguang , Wang Yinglong , Zhu Zhaoyou , Li Guoxuan 
Published: December 2024

AbstractA novel phospho‐based hydrophobic deep eutectic solvents (HDESs) is proposed to selectively extract valuable metals from waste lithium‐ion batteries (LIBs). Under the optimized extraction conditions, the single‐stage extraction efficiency of HDES [TOP][Lid] for Co2+ and Ni2+ were 98.5% and 83.9%, and HDES [TBP][Lid] for Co2+ and Ni2+ were 96.0% and 82.9%, and Li+ was enriched in the extract. FT‐IR, 1H NMR, and ESP analysis confirmed the hydrogen bond between HBD and HBA. The metal ion extraction mechanism by HDESs was analyzed based on quantum chemistry (QC) and molecular dynamics (MD). The extraction mechanism at the molecular level is that electrostatic and coordination interactions between transition metal ions and HDESs dominate the extraction of metal ions (Co2+ and Ni2+). The interaction intensity with HDESs was stronger than that between Li+ and HDESs.
Peripheral risk factors and their role in biomarker‐based screening for dementia in the community
Authors: Hu Yi‐Han , Meirelles Osorio , Shiroma Eric J. , Satizabal Claudia L. , Seshadri Sudha , Tracy Russell P. , Gudnason Vilmundur , Launer Lenore J. 
Published: December 2024

AbstractINTRODUCTIONPeripheral risk factors (PRFs) may correlate with dementia plasma biomarkers, potentially reflecting peripheral rather than brain health. This study explores the associations between PRFs and plasma biomarkers glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), and total‐tau, and their role in predicting future dementia.METHODSData from the Age, Gene/Environment Susceptibility–Reykjavik Study (2002–2015) included 4353 participants mean age of 76.6 years. A subsample of 910 participants tested their association with PRFs and plasma biomarkers’ predictive performance. Sociodemographic, clinical, laboratory, sensory, and lifestyle variables (n = 305) were grouped into 34 clusters.RESULTSBesides age and estimated glomerular filtration rate (eGFR), significant associations were found between plasma biomarkers and clusters related to hemoglobin, red blood cell distribution, and inflammation. Incorporating these clusters into predictive models enhanced precision and sensitivity, though overall prediction improvement was modest (area under the precision‐recall curve: GFAP 0.17 to 0.34, NfL 0.20 to 0.38).DISCUSSIONPRFs are significantly associated with dementia plasma biomarkers; Considering these factors may enhance the predictive accuracy of dementia biomarkers.Highlights
Machine learning identifies key peripheral factors influencing neurodegenerative biomarkers.
Hemoglobin and red blood cell distribution cluster associates significantly with biomarker levels.
Incorporating diverse peripheral factors modestly enhances incident dementia prediction accuracy in community settings.
Alkene Epoxidation with Water by Confined Active Co Sites on BiVO4 Photoanodes under Visible Light
Authors: Wu Haisu , Wang Yankun , Huang Meirong , Cheng Jiajia , Sa Baisheng , Fang Yuanxing , Wang Xinchen 
Published: December 2024

AbstractHeterogeneous photoelectrocatalysis systems have recently seen significant growth in organic transformations, but are limited by the inherent physicochemical properties of electrode materials. To enhance selectivity in these processes, we propose an innovative advancement in the rational design of photoanodes. Specifically, we incorporated cobalt porphyrin co‐catalysts with confined Co sites onto bismuth vanadate films as a photoanode. This photoanode significantly enhances the efficacy of styrene epoxidation, achieving selectivity and conversion rates of 90 % and 99 %, respectively. Notably, the reaction utilizes water as the sole oxygen source, operates at room temperature, and is easily scalable for gram‐scale synthesis. The developed photoanode demonstrates robust performance across various alkene substrates. Operando characterizations reveal that during the epoxidation reaction, the confined Co sites within the porphyrin structure catalyze the oxidation of H2O to form Co−O*, serving as critical intermediates that facilitate cyclization reactions via one‐electron processes. This study introduces an innovative heterogeneous photoelectrocatalysis strategy with customizable active sites tailored for selective catalytic organic transformations.
Solvent‐Free Chemical Recycling of Polyesters and Polycarbonates by Magnesium‐Based Lewis Acid Catalyst
Authors: Zhao Wuchao , Guo Zongpeng , He Jianghua , Zhang Yuetao 
Published: December 2024

AbstractDeveloping a simple and efficient catalyst system for closed‐loop recycling of polymers to monomers is an essentially important but challenging task for the recycle of polymer wastes and preventing the downcycle of plastic products. Herein, we employ inexpensive, commercially available Lewis acids (LAs) to achieve closed‐loop recycling in bulk through the catalytic depolymerization of aliphatic polyesters and polycarbonates. The scope of LAs is screened by thermogravimetric analysis experiments and distillation experiments. MgCl2 shows the best catalytic performance that can efficiently depolymerize eleven aliphatic polyesters and polycarbonates (i.e. poly(ϵ‐caprolactone) and poly(trimethylene carbonate)), into monomers (up to 98 % yield) at temperatures significantly lower than the ceiling temperature. Moreover, this catalyst system exhibits high selectivity and compatibility towards the depolymerization of (co)polyesters as well as the blends of polyesters and polycarbonates in the presence of other commodity plastics, as well as excellent recycle and reuse catalyst performance. Mechanistic studies indicate that the closed‐loop recycling of monomers is achieved through the random chain scission and terminal group cyclization.
Design, Synthesis, and Antibacterial Activities of Multi‐Functional C2‐Functionalized 1,4‐Naphthoquinonyl Organoseleniums
Authors: Jha Raushan Kumar , Chhavi  , Jaiswal Svastik , Parganiha Devendra , Choudhary Vishal , Saxena Deepanshi , Maitra Rahul , Singh Swechcha , Chopra Sidharth , Kumar Sangit 
Published: December 2024

AbstractA practical and efficient reaction for C2‐selenylation of 1,4‐naphthoquinones has been explored. This coupling reaction of two redox structural motifs, such as 2‐bromo‐1,4‐naphthoquinone with diaryldiselenide/ebselen has been achieved by using sodium borohydride reducing agent at room temperature. Using this approach, several 2‐selenylated‐1,4‐naphthoquinones were obtained in moderate to good yields and thoroughly characterized by multinuclear (1H, 13C, and 77Se) NMR, cyclic voltammetry, and mass spectrometry. Further, light‐irradiated thiolation of the synthesized selenazinone was also performed to show the utility of the synthesized compound for post‐functionalization. Several 2‐selenylated‐1,4‐naphthoquinones were studied by SC‐XRD in which intramolecular Se⋅⋅⋅N (from quinolinyl ligand) non‐bonded interactions were observed. Photophysical studies (UV‐visible, emission, solvatochromism, and quantum yield) were also performed on selected C2‐selenylated naphthoquinones. The naphthoquinonyl organoseleniums were also screened for their antibacterial properties and quinonyl organoselenium 5 d shows good antibacterial potential against S. aureus ATCC 29213 with MIC 0.5 μg/mL and a Selectivity Index of >200. Moreover, it also exhibited equipotent activity against various strains of S. aureus and Enterococcus faecium, including strains resistant to vancomycin and meropenem. From structure‐activity correlation, it seems that nice blend of oxidant properties from quinone and antioxidant properties from selenium moiety makes it better candidate for antibacterial activity.
Saving a hopeless tooth with a four‐wall bone defect: A case report
Authors: Nagahara Takayoshi , Iwata Tomoyuki , Wada Keinoshin , Ueda Tomoya , Kono Shoko , Ouhara Kazuhisa , Mizuno Noriyoshi 
Published: December 2024

AbstractBackgroundRecombinant human fibroblast growth factor‐2 (rhFGF‐2) has been shown to effectively promote the formation of new periodontal tissues, and its efficacy has been demonstrated in clinical settings. Moreover, the clinical and radiographic outcomes in the treatment of periodontal infrabony defects can be improved by using rhFGF‐2 in combination with a bone substitute. Here, we present a case of four‐wall bone defect in a tooth treated by combination regenerative therapy using rhFGF‐2 and beta‐tricalcium phosphate (β‐TCP).MethodsA 43‐year‐old male with a four‐wall bone defect in tooth #28 was subjected to combination therapy with rhFGF‐2 and β‐TCP. Periodontal clinical parameters and radiographic images were evaluated at the first visit, after the initial periodontal treatment, and after 4 months and 4 years postoperation.ResultsAlthough gingival recession and nonvital pulp were observed postoperation, improvements in the periodontal parameters and radiographic outcomes were subsequently recorded.ConclusionPeriodontal regenerative therapy with a combination of rhFGF‐2 and β‐TCP showed great potential in the treatment of four‐wall bone defects of teeth.Key points
Periodontal regenerative therapy using recombinant human fibroblast growth factor‐2 (rhFGF‐2) and beta‐tricalcium phosphate (β‐TCP) showed immense potential in the treatment of four‐wall bone defect in teeth.
In addition to using computed tomography for assessing bone defects and root morphologies, an evaluation of root canal morphology and pulp diagnosis is essential for understanding the internal and external aspects of the defect, which would aid in tooth preservation.
Appropriate periodontal and endodontic treatments enabled tooth preservation following apical periodontitis after periodontal regenerative therapy.
Plain language summaryThis case report focuses on a new approach for the treatment of a four‐wall bone defect, a significant dental issue in which the bone around the tooth is damaged, thereby decreasing the stability of the tooth. We treated a 43‐year‐old male patient with a combination of two key components: the growth factor rhFGF‐2, which helps promote the growth of new tissues, and the synthetic bone graft substitute β‐TCP, which acts as a substitute for the missing bone. Over the course of 4 years, we monitored the patient's progress using dental exams and X‐ray photos. Despite some minor side effects, such as gum recession and the loss of tooth vitality, the overall condition of the tooth and surrounding bone showed significant improvement. This combination therapy shows promise in repairing similar bone defects, which would help save teeth that might otherwise be lost.
Low Parental Empathy Predicts Callous‐Unemotional Traits in Children With Enhanced Autonomic Sensitivity to the Environment
Authors: Kofler Liat , Raine Adrian , Gao Yu 
Published: December 2024

ABSTRACTIntroductionRecent evidence suggests that autonomic nervous system functioning can reflect individual differences in sensitivity to the environment, which in turn moderates the effects of family context on psychopathic and antisocial behaviour. Although some preliminary research suggests that the coordination of the sympathetic (SNS) and parasympathetic (PNS) branches of the autonomic nervous system may reflect sensitivity to environmental influences, the majority of research to date has been limited in its focus on just one branch, and little is known about the effects of parental empathy on a child's psychopathic‐like traits. This study aimed to address this gap by examining if the SNS and PNS jointly moderate the prospective contribution of parental empathy to youth psychopathic‐like traits.MethodsMale and female children (n = 340; age = 8–10 years) from the community completed an emotion regulation task during which their skin conductance level (SCL) and respiratory sinus arrhythmia (RSA) activity were recorded. Parental cognitive, affective, and somatic empathy were reported by the caregivers. The child's psychopathic‐like traits were reported by the caregiver and the child and were reassessed 1 year later.ResultsHierarchical regression indicated that after controlling for child sex, age, and concurrent CU traits, low affective empathy in parents predicted higher CU traits 1 year later in youths who exhibited reciprocal sympathetic activation (i.e., SNS activation along with PNS withdrawal), reflecting their enhanced sensitivity to the environment. No such effects were found for other psychopathic‐like traits.ConclusionsFindings suggest that reciprocal SNS reactivity may be a biological indicator for sensitivity to environmental influence and highlight the importance of examining the interactions among multiple systems to better understand the aetiology of psychopathic traits.
A Systematic Review and Meta‐Analysis of the Efficacy and Safety of Combined Mechanical Circulatory Support in Acute Myocardial Infarction Related Cardiogenic Shock
Authors: Soh Bing Wei Thaddeus , Gracias Carlos Sebastian , Dean Afshan , Kumar Jathinder , Asgedom Solomon , Matiullah Sajjad , Owens Patrick 
Published: December 2024

ABSTRACTBackgroundAcute myocardial infarction‐related cardiogenic shock (AMICS) is a severe complication associated with exceedingly high mortality rates. While mechanical circulatory support (MCS) has emerged as a potential intervention, the evidence base for independent MCS use remains weak. In contrast, systematic reviews of observational studies have revealed significant mortality reduction when a combination of MCS was used: VA‐ECMO in conjunction with a left ventricular (LV) unloading device (Impella or IABP). The ongoing dilemma concerning the selection between two LV unloading devices (VA‐ECMO + Impella vs. VA‐ECMO + IABP) warrants further investigation and clarification.AimThis is the first systematic review and meta‐analysis assessing the short‐term efficacy and safety of VA‐ECMO + Impella versus VA‐ECMO + IABP in treatment of AMICS.MethodsA systematic search was performed on the EMBASE, MEDLINE, and Cochrane databases. Studies reporting the short‐term (30‐day/inpatient) mortality and complications of adult patients with AMICS treated with VA‐ECMO + Impella and VA‐ECMO + IABP were included. Subgroup analysis was performed including studies with ACS predominant CS (CS etiology 100% by AMI).ResultsFour observational studies with 14,247 patients were included. There was no significant difference in mortality between VA‐ECMO + Impella and VA‐ECMO + IABP (56.5% vs. 66.5%; OR, 0.90; 95% CI, 0.79−1.02; p = 0.09). However, VA‐ECMO + Impella was associated with significantly lower mortality in patients with ACS predominant CS (53.2% vs. 67.7%; OR, 0.72; 95% CI, 0.62−0.85; p < 0.0001). VA‐ECMO + Impella was concomitantly associated with a significantly higher risk of complications.ConclusionsWhen comparing LV unloading devices in patients with AMICS requiring a combination of MCS, VA‐ECMO + Impella was superior in mortality reduction only in the cohort where 100% of CS was caused by AMI.
Searching and Using MobiDB Resource 6 to Explore Predictions and Annotations for Intrinsically Disordered Proteins
Authors: Aspromonte Maria Cristina , Quaglia Federica , Monzon Alexander Miguel , Clementel Damiano , Del Conte Alessio , Piovesan Damiano , Tosatto Silvio C. E. 
Published: December 2024

AbstractIntrinsically disordered proteins (IDPs) make up around 30% of eukaryotic proteomes and play a crucial role in cellular processes and in pathological conditions such as neurodegenerative disorders and cancers. However, IDPs exhibit dynamic conformational ensembles and are often involved in the formation of biomolecular condensates. Understanding the function of IDPs is critical to research in many areas of science. MobiDB is a unique resource that serves as a comprehensive knowledgebase of IDPs and intrinsically disordered regions (IDRs), combining disorder annotations from experimental evidence and predictions for a broad range of protein sequences. Over the past decade, MobiDB has evolved with a focus on expanding annotation coverage, standardizing annotation provenance, and enhancing database accessibility. The latest MobiDB, version 6, released in July 2024, includes significant improvements, such as the integration of AlphaFoldDB predictions and a new homology transfer pipeline that has substantially increased the number of entries with high‐quality annotations. The user interface has also been updated, highlighting annotation features, clarifying the entry page, and providing an immediate overview of disorder, binding, and disorder functions information in the protein sequence. This protocol guides the user through applications of the MobiDB, including disorder prediction, curated data analysis, and exploration of interaction data. This guide covers how to perform a search in MobiDB annotations using the web interface and the MobiDB REST API for programmatic access. The protocols use a step‐by‐step walkthrough using the human growth hormone receptor to demonstrate MobiDB's functions for visualization and interpretation of protein disorder data. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Searching MobiDB query formatsBasic Protocol 2: Searching MobiDB selected datasets and selected proteomesBasic Protocol 3: Performing a search on the Statistics page in MobiDBSupport Protocol: Programmatic access with MobiDB REST APIBasic Protocol 4: Visualizing and interpreting a MobiDB Entry: The GHR use case