bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Komagataeibacter europaeus GqqA is the prototype of a novel bifunctional N-Acyl-homoserine lactone acylase with prephenate dehydratase activity
Authors: Werner Nadine , Petersen Katrin , Vollstedt Christel , Garcia Pablo Perez , Chow Jennifer , Ferrer Manuel , Fernandes-Lopez Laura , Falke Sven , Perbandt Markus , Hinrichs Winfried , Betzel Christian , Streit Wolfgang R. 
Published: March 2021

Abstract
Previously, we reported the isolation of a quorum quenching protein (QQ), designated GqqA, from Komagataeibacter europaeus CECT 8546 that is highly homologous to prephenate dehydratases (PDT) 1. GqqA strongly interfered with N-acyl-homoserine lactone (AHL) quorum sensing signals from Gram-negative bacteria and affected biofilm formation in its native host strain Komagataeibacter europaeus. Here we present and discuss data identifying GqqA as a novel acylase. ESI-MS-MS data showed unambiguously that GqqA hydrolyzes the amide bond of the acyl side-chain of AHL molecules, but not the lactone ring. Consistent with this observation the protein sequence does not carry a conserved Zn2+ binding motif, known to be essential for metal-dependent lactonases, but in fact harboring the typical periplasmatic binding protein domain (PBP domain), acting as catalytic domain. We report structural details for the native structure at 2.5 Å resolution and for a truncated GqqA structure at 1.7 Å. The structures obtained highlight that GqqA acts as a dimer and complementary docking studies indicate that the lactone ring of the substrate binds within a cleft of the PBP domain and interacts with polar residues Y16, S17 and T174. The biochemical and phylogenetic analyses imply that GqqA represents the first member of a novel type of QQ family enzymes.
Errors linked to medication management in nursing homes: An interview study
Authors: Bengtsson Mariette , Ekedahl Ann-Britt Ivarsson , Sjöström Karin 
Published: March 2021

Abstract
Background: The number of errors in medication management in nursing homes is increasing, which may lead to potentially life-threatening harm. Few studies on this subject are found in the municipal nursing home setting, and causes need to be identified. The aim of this study was to explore perceptions of errors connected to medication management in nursing homes by exploring the perspective of first-line registered nurses, registered nurses, and non-licensed staff involved in the care of older persons. Methods: A qualitative research approach was applied based on semi-structured interviews with 21 participants at their workplaces: Seven in each of the occupational categories of first-line registered nurses, registered nurses and non-licensed staff. Subcategories were derived from transcribed interviews by content analysis and categorized according to the Man, Technology, and Organization concept of error causation, which is as a framework to identifiy errors.Results: Mistakes in medication management were commonly perceived as a result of human shortcomings and deficiencies in working conditions such as the lack of safe tools to facilitate and secure medication management. The delegation of drug administration to non-licensed staff, the abandonment of routines, carelessness, a lack of knowledge, inadequate verbal communication between colleagues, and a lack of understanding of the difficulties involved in handling the drugs were all considered as risk areas for errors. Organizational hazards were related to the ability to control the delegation, the standard of education, and safety awareness among staff members. Safety issues relating to technology involved devices for handling prescription cards and when staff were not included in the development process of new technological aids. A lack of staff and the lack of time to act safely in the care of the elderly were also perceived as safety hazards, particularly with the non-licensed staff working in nursing homes.Conclusions: The staff working in nursing homes perceive that the risks due to medication management are mainly caused by human limitations or technical deficiencies. Organizational factors, such as working conditions, can often facilitate the occurrence of malpractice. To minimize mistakes, care managers need to have a systemwide perspective on safety issues, where organizational issues are essential.
Single-sequence probes reveal chromosomal locations of tandemly repetitive genes in scleractinian coral Acropora pruinosa: a potential tool for karyotyping
Authors: Vacarizas Joshua , Taguchi Takahiro , Mezaki Takuma , Okumura Masatoshi , Kawakami Rei , Ito Masumi , Kubota Satoshi 
Published: March 2021

Abstract
The short and similar sized chromosomes of Acropora pose a challenge for karyotyping. Conventional methods, such as staining of heterochromatic regions, provide unclear banding patterns that hamper identification of such chromosomes. In this study, we used short single-sequence probes for tandemly repetitive 5S ribosomal RNA (rRNA) and core histone genes to identify specific chromosomes of Acropora pruinosa. Both the probes produced intense signals in fluorescence in situ hybridization, which distinguished chromosome pairs. The locus of the core histone gene was on chromosome 8, whereas that of 5S rRNA gene was on chromosome 5. The sequence of the 5S rRNA probe was composed largely of U1 and U2 spliceosomal small nuclear RNA (snRNA) genes and their interspacers, flanked by short sequences of the 5S rRNA gene. This is the first report of a tandemly repetitive linkage of snRNA and 5S rRNA genes in Cnidaria. Based on the constructed tentative karyogram and whole genome hybridization, the longest chromosome pair (chromosome 1) was heteromorphic. The probes also hybridized effectively with chromosomes of other Acropora species and population, revealing an additional core histone gene locus. We demonstrated the applicability of short-sequence probes as chromosomal markers with potential for use across populations and species of Acropora.
Genetic basis of some physiological traits and their association with yield in pre-breeding germplasm of bread wheat (Triticum aestivm L.)
Authors: Arif Mian Abdur Rehman , Imran Muhammad , Waheed Muhammad Qandeel , Arif Anjuman , Singh Sukhwinder , Shokat Sajid 
Published: March 2021

Abstract
The rise in human population necessitates the use of all available tools to enhance wheat productivity. In this regard, pre-breeding has mobilized novel under-utilized genetic variation into breeding programs. However, this germplasm needs to be characterized for its efficient utlization. This investigation was initiated to evaluate the early and late sown wheat pre-breeding germplasm for physiology and yield related traits and to associate the mapped SNPs using association mapping approach. Our results indicate that the germplasm performed better in early sowing in comparison to late planting where grain yield (Yd) was found positively correlated with water use efficiency (WUE), heading time and chlorophyll contents (Chl). We discovered a total of 210 associations involving 155 SNPs. Taking into consideration either early or late sowing and the mean values only 12 markers traits were involved with germination, plant height, stomatal conductance, transpiration rate, Chl, carotenoids and Yd. Our correlations and mapping results indicate that higher WUE along-with Chl can be targeted as indirect physiological markers to enhance wheat yield.
Influence of hydrological factors on bacterial community structure in a tropical monsoonal estuary in India
Authors: Ammini Parvathi , Catena Michela R , Vijayan Jasna , Phadke Nikhil , Gogate Nikhita 
Published: March 2021

Abstract
In the present study, we analysed variations in bacterial community structure along a salinity gradient in a tropical monsoonal estuary (Cochin estuary, CE), on the southwest coast of India, using Illumina next-generation sequencing (NGS). Water samples were collected from eight different locations thrice a year, to assess the variability in the bacterial community structure and to determine the physico-chemical factors influencing the bacterial diversity. Proteobacteria was the most dominant phyla in the estuary followed by Bacteroidetes, Cyanobacteria, Actinobacteria, and Firmicutes. Statistical analysis indicated significant variations in bacterial communities between freshwater, mesohaline and euryhaline regions, as well as between the monsoon (wet) and non-monsoon (dry) periods. Non-metric multidimensional scaling (NMDS) analysis demonstrated that the bacterial communities cluster according to different salinity regimes of the estuary. Canonical Correspondence analysis (CCA) showed a clear spatial and temporal variation in the distribution of bacterial communities in the CE. Abundance of Betaproteobacteria was high in the freshwater regions, while Gammaproteobacteria, Alphaproteobacteria and Epsilonproteobactera were more abundant in mesohaline and euryhaline regions of the estuary. Correlagram based on Pearson correlation analysis demonstrated the impact of different physico-chemical variables on the distribution of dominant phyla, class and genera. Spatial and temporal variations in bacterial community structure could be due to regional variations in environmental conditions imparted by allochthonous inputs, monsoonal rainfall, and tidal influence.
Are Caveolin-1 minor alleles more likely to be risk alleles in IR mechanisms in metabolic diseases?
Authors: Abaj Faezeh , Saeedy Said Abdul Ghafour , Mirzaei Khadijeh 
Published: March 2021

Abstract
Objectives

Associations are present between insulin resistance (IR) and dyslipidemia and cardiometabolic factors. Caveolin-1 (CAV1) is involved in glucose/lipid homeostasis and may modulate IR signaling. We investigated the relationship between CAV1 and IR signaling in modulating dyslipidemia and fat composition in overweight and obese women with a prevalent variant in the CAV1 gene.
Results

There were no statistical differences in FPG, plasma insulin, and HOMA-IR (p > 0.05) between CAV1 variants. Individuals with AA and AG alleles were slightly older and had higher BMI, FMI, and VLF values; and tended to have lower total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) (p < 0.05). HOMA-IR levels predicted fat mass index (FMI) 0.47 (0.08,0.87), visceral fat level (VFL) 0.65 (0.23,1.07), TC 6.82 (1.76,11.88) and HDL-C -1.663 (-3.11, -0.214) only between minor allele carriers in unadjusted and adjusted models. (, CI (P < 0.01). Our r
Establishment of dopaminergic neuron purification system in mice for the Parkinson’s disease study
Authors: Sheng Kit-Yeng , Hayakawa Hideki , Baba Kousuke , Kimura Yasuyoshi , Mochizuki Hideki , Nakano Toru , Yamaguchi Shinpei 
Published: March 2021

Abstract
Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the degeneration of dopaminergic (DA) neurons. The key neuropathological hallmarks in the brain of patients with PD are Lewy body (LB) inclusions, consisting of misfolded α-synuclein proteins. Despite extensive efforts, the molecular link between LB inclusions and DA neurodegeneration remains elusive because of the lack of a suitable approach. Here, we aimed to establish a novel dopa-decarboxylase (Ddc) fluorescent reporter mouse model that allows the identification and collection of DA neurons using a fluorescence-activated cell sorter. Successful enrichment of Ddc-expressing cells was validated by RNA-sequencing analysis. This approach allowed us to analyze the effect of α-synuclein accumulation on the DA neuron’s transcriptome prior to neurodegeneration occurrence. We found that lipid-related process genes, followed by protein modification and degradation-related process genes, were upregulated in the α-synuclein-injected DA neurons. The activation of fatty acid-binding protein 1 (Fabp1) was particularly evident and confirmed by immunohistochemistry. Thus, our mouse model system and datasets provide a new method and insights into molecular mechanisms in PD.
PENGARUH PENAMBAHAN BAUT DAN PAKU TERHADAP KUAT LENTUR BALOK LAMINASI KAYU MAHANG DAN MERANTI
Authors: Puluhulawa Indriyani , Alamsyah Alamsyah , Rafika Diana , Khoirunisak Khoirunisak 
Published: March 2021

ABSTRACT The addition of bolts and nails as a shear connector on the laminate beam was expected to anticipate the problem on the laminate beam which only uses glue adhesive. The samples were a specimen of material properties and a specimen of the laminated beam, the dimension was 76 x 5 x 5 cm with three types of adhesive variation. The MOR value was obtained by one point load on the middle of the span. The value was the load and deflection, further calculated the value to get MOR and MOE. The MOR result of the addition of nail as the connector on the laminate beam by glue was increased 36.9%. In contrast, the addition of bolt was decreased 49.86% compared to MOR BL LM. The addition of nails and bolts to laminated beams that used glue already showed the effect to decrease the MOE value of the laminate beam.Keywords: Connector bolt and nail, Flexural strength, Laminate beam. ABSTRAK Penambahan baut dan paku sebagai perekat diharapkan dapat mengantisipasi masalah yang umum terjadi pada balok laminasi yang hanya menggunakan perekat lem. Sampel yang dibuat meliputi sampel properties material serta sampel balok laminasi berukuran 76 x 5 x 5 cm sebanyak 3 tipe variasi jenis perekat. Nilai MOR diperoleh dengan pemberian beban satu titik ditengah bentang. Nilai yang diambil adalah beban dan lendutan, yang kemudian dihitung untuk mendapatkan nilai MOR dan MOE. Hasilnya penambahan paku sebagai penghubung/perekat pada balok laminasi yang menggunakan lem, dapat meningkatkan nilai MOR sebesar 36,9%, sedangkan penambahan baut menyebabkan penurunan nilai MOR sebesar 49,86% dibandingkan terhadap nilai MOR BL LM. Penambahan paku dan baut pada balok laminasi yang menggunakan lem juga dapat menurunkan nilai MOE dari balok laminasi itu sendiri. Kata kunci: Perekat baut dan paku, Kuat lentur, Balok laminasi.
PENATAAN SISTEM JALUR PEJALAN KAKI DI UNIVERSITAS NEGERI YOGYAKARTA
Authors: Agphin Ramadhan Muhammad , Nur Indriatno Putra Pratama Galeh , Hidayah Retna 
Published: March 2021

ABSTRACTThis study aims to: 1) determine the zoning for pedestrian ways; 2) determine the type of pedestrian ways in UNY; 3) to identify problems and solutions related to pedestrian ways in UNY. The method used in this research is descriptive with quantitative approach, by comparing the standard criteria for open space with the condition of reality. The technique of collecting data through observation and measurement of the dimensions of buildings and open spaces using the assistance planimeter website. Basic physical reviews grouping zones through which the road that separates the faculty contained in UNY. The results showed that: 1) zoning is physically in the Campus UNY consists of six zones, namely: Zone A (FT, LPPMP, LPPM), Zone B (FE, FIS, Dean Faculty, Kopma, PPs Lama, FIP), zone C (FBS, Science Faculty, Masjid Mujahideen), zone D (GOR UNY and vicinity), zone E (Stadiums, Tennis Court, FIK), and zone F (Rector, New PPs); 2) in real terms, there are four types of pedestrian ways in UNY, namely: Type 1 (separated), Type 2 (integration), Type 3 (dominant car), and Type 4 (corridor); 3) alternative solutions for problems of special dimensions and availability (level of service) the dimensions sidewalk pavement is in zone B is not ideal, while the sidewalks in zone A is sufficient available. Keywords: Pedestrian, Sidewalk, Yogyakarta State University. ABSTRAKKajian ini bertujuan untuk: 1) mengetahui pembagian zona untuk pedestrian ways; 2) mengetahui tipe pedestrian ways di UNY; 3) mengetahui permasalahan dan solusi yang berhubungan dengan pedestrian ways di UNY. Metode yang digunakan dalam kajian ini adalah deskriptif melalui pendekatan kuantitatif, dengan cara membandingkan kriteria standar ruang terbuka dengan kondisi realita. Teknik pengumpulan data melalui observasi dan pengukuran dimensi bangunan serta ruang terbuka menggunakan bantuan website planimeter. Dasar pengelompokkan zona melalui tinjauan fisik yaitu jalan yang memisahkan beberapa Fakultas yang terdapat di UNY. Hasil kajian menunjukkan bahwa: 1) pembagian zona secara fisik di kawasan Kampus UNY terdiri dari 6 zona, yaitu: Zona A (FT, LPPMP, LPPM), Zona B (FE, FIS, Dekanat FMIPA, Kopma, PPs Lama, FIP), Zona C (FBS, FMIPA, Masjid Mujahiddin), Zona D (GOR UNY dan sekitarnya), Zona E (Stadion, Lapangan Tenis, FIK), dan Zona F (Rektorat, PPs Baru); 2) secara riil, ada 4 tipe pedestrian ways di UNY, yaitu: Tipe 1 (separated), Tipe 2 (integration), Tipe 3 (car dominant), dan Tipe 4 (corridor); 3) alternatif solusi atas permasalahan khusus dimensi dan ketersediaan (tingkat pelayanan) trotoar yaitu dimensi trotoar yang berada di zona B belum ideal, sedangkan trotoar pada zona A sudah cukup tersedia. Kata kunci: Pedestrian, Trotoar, Universitas Negeri Yogyakarta.
AKURASI PREDIKSI DURASI PENYELESAIAN PROYEK DENGAN EARN VALUE MANAGEMENT DAN EARN SCHEDULE (STUDI KASUS PROYEK BANGUNAN GEDUNG DI UNESA)
Authors: Agus Yudha Prawira Adistana Gde , Mahardi Purwo , Firmansyah Sofianto Mochamad , Eksana Wibowo Dian 
Published: March 2021

ABSTRACT The earn value method (EVM) has been widely recognized and proven to provide an overview of the performance of construction project implementation from the aspects of time and schedule. Specifically EVM is used to evaluate the progress of the work, so identification of deviations both on time and cost can be done early.The Earn Schedule (ES) method is an elaboration to the schedule analysis on the EVM method. This study aimed to examine and compare the capability of the two methods, namely the earn value and earn schedule methods in predicting the completion time of construction project. The research used the real data from the Unesa building project. The results showed that at week 9, the value of CPI was 1.15 or had a variant cost of 13.32% lower than the planned budget. While for the schedule aspect, both methods indicated a delay in the project with the delay variant of 0.16%. The ES method is able to give information of how long the delay in time unit of 0.02 week.Keywords: accuration, actual cost, earn value, earn schedule, planed value ABSTRAKMetode nilai hasil (EVM) telah dikenal luas dan terbukti mampu memberikan gambaran kinerja pelaksanaan proyek konstruksi dari aspek waktu dan jadwal. Secara khusus EVM digunakan untuk mengevaluasi progress pekerjaan sehingga dapat dilakukan identifikasi lebih awal terhadap penyimpangan baik terhadap waktu maupun biaya. Metode Earn Schedule (ES) merupakan pendalaman terhadap analisis jadwal pada metode EVM. Penelitian ini dilakukan untuk menguji dan membandingkan kemampuan dua metode yaitu metode earn value dan earn schedule dalam memprediksi waktu penyelesaian proyek konstruksi. Data penelitian menggunakan data pelaksanaan dari pembangunan proyek bangunan gedung di Unesa. Hasil penelitianmenunjukkan bahwa pada minggu ke-9, nilai CPI adalah sebesar 1,15 atau memiliki varian biaya sebesar 13,32% lebih rendah dari anggaran yang direncanakan. Sedangkan dari aspek jadwal, kedua metode mengindikasikan bahwa terjadi keterlambatan pada proyek yaitu dengan varian keterlambatan sebesar 0,16% . Metode ES mampu memberikan informasi berapa lama keterlambatan yang terjadi dalam satuan waktu yaitu sebesar 0,02 minggu. Kata Kunci: akurasi, biaya aktual, nilai hasil, earn schedule, biaya rencana
Commitment Levels of Health Care Providers in Using the District Health Information System and the Associated Factors for Decision Making in Resource-Limited Settings: Cross-sectional Survey Study
Authors: Kanfe Shuma G , Endehabtu Berhanu F , Ahmed Mohammedjud H , Mengestie Nebyu D , Tilahun Binyam 
Published: March 2021

Background
Changing the culture of information use, which is one of the transformation agendas of the Ministry of Health of Ethiopia, cannot become real unless health care providers are committed to using locally collected data for evidence-based decision making. The commitment of health care providers has paramount influence on district health information system 2 (DHIS2) data utilization for decision making. Evidence is limited on health care providers’ level of commitment to using DHIS2 data in Ethiopia. Therefore, this study aims to fill this evidence gap.

Objective
This study aimed to assess the levels of commitment of health care providers and the factors influencing their commitment levels in using DHIS2 data for decision making at public health care facilities in the Ilu Aba Bora zone of the Oromia national regional state, Ethiopia in 2020.

Methods
The cross-sectional quantitative study supplemented by qualitative methods was conducted from February 26, 2020 to April 17, 2020. A total of 264 participants were approached. SPSS version 20 software was used for data entry and analysis. Descriptive and analytical statistics, including bivariable and multivariable analyses, were performed. Thematic analysis was conducted for the qualitative data.

Results
Of the 264 respondents, 121 (45.8%, 95% CI 40.0%-52.8%) respondents showed high commitment levels to use DHIS2 data. The variables associated with the level of commitment to use DHIS2 data were found to be provision of feedback for DHIS2 data use (adjusted odds ratio [AOR] 1.85, 95% CI 1.02-3.33), regular supervision and managerial support (AOR 2.84, 95% CI 1.50-5.37), information use culture (AOR 1.92, 95% CI 1.03-3.59), motivation to use DHIS2 data (AOR 1.80, 95% CI 1.00-3.25), health needs (AOR 3.96, 95% CI 2.11-7.41), and competency in DHIS2 tasks (AOR 2.41, 95% CI 1.27-4.55).

Conclusions
In general, less than half of the study participants showed high commitment levels to use DHIS2 data for decision making in health care. Providing regular supportive supervision and feedback and increasing the motivation and competency of the health care providers in performing DHIS2 data tasks will help in promoting their levels of commitment that can result in the cultural transformation of data use for evidence-based decision making in health care.
History of Sheath Rot Disease in Indonesia and Disease Severity in Two Rice Production Centres of West Java
Authors: Afifah Khoirotul , Wiyono Suryo , Yuliani Titiek Siti , Wibowo Baskoro Sugeng 
Published: March 2021

The observation conducted from 2000 to 2018 found that rice sheath rot has become an emerging disease in several regions. The disease was able to reduce quality and quantity of rice yield. This research objective was to study the history and status of rice sheath rot caused by Sarocladium oryzae. Data was obtained from literature study, farmer interview, and field survey. Data were used to calculate disease incidence followed by visual observation of the symptoms caused by this disease. Identification of sheath rot pathogen was done using microscopic and molecular techniques using specific primers. Rice sheath rot was first reported in Indonesia as a minor disease in 1987 and has only currently become an important disease. This fungus generally causes rice sheath rot in Karawang and Cianjur Regency. Disease incidence of rice sheath rot was 12.56% without considering the varieties at all generative growth stages. Average disease incidence on all rice varieties observed was 12.64%, except for IR 42 (0%). The level of rice plants damage due to rice sheath rot in Cianjur was higher than Karawang with average disease incidence of 11.58% and 9.27%, respectively. Rice sheath rot symptoms have often been found in the last 10 years with average level of damage of 3%‒18% and yield loss of 10%-15%. This disease has proven to be important and has become a challenge in rice cultivation. The fungus that infect rice plants in Karawang and Cianjur had different morphotypes, namely KP, KP2, KP3, PW3, and PW03. The amplification results showed that all fungal isolates were S. oryzae.