bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Expression of PPFIA1 Is Associated With Tumor Progression and a Poor Prognosis in Esophageal Squamous Cell Carcinoma
Authors: Zhang Hongdian , Jia Ran , Yang Yueyang , Sui Zhilin , Xiao Wanyi , Wu Xianxian , Gong Lei , Yu Zhentao , Tang Peng 
Published: May 2021

Abstract
Background: PTPRF interacting protein alpha 1 (PPFIA1) is reportedly related to the occurrence and progression of several types of malignancies. However, its role in esophageal squamous cell carcinoma (ESCC) remains unknown. We aimed to investigate the expression and clinical value of PPFIA1 in ESCC.Methods: The Oncomine, Gene Expression Profiling Enrichment Analysis (GEPIA), and Gene Expression Omnibus (GEO) databases were utilized to explore PPFIA1 mRNA expression in esophageal cancer. The associations of PPFIA1 expression with clinicopathological variables and prognosis were evaluated in the GSE53625 dataset and verified in quantitative real-time polymerase chain reaction (qRT-PCR)-based cDNA array and immunohistochemistry (IHC)-based tissue microarray (TMA) datasets. The interactions between PPFIA1 and other genes based on the protein-protein interaction (PPI) network was analyzed via the STRING website.Results: PPFIA1 expression was obviously upregulated in ESCC tissues versus adjacent normal tissues according to online database analyses (all P<0.05). High PPFIA1 expression was significantly associated with several clinicopathological features, including tumor size, histological grade, tumor invasion depth, lymph node metastasis, and tumor-node-metastasis (TNM) stage. High PPFIA1 expression was related to worse outcomes and was identified as an independent prognostic indicator of overall survival (OS) in ESCC patients GSE53625 dataset, P=0.004; cDNA array dataset, P<0.001; TMA dataset, P=0.039). PPI analysis demonstrated that PPFIA1 was highly correlated with multiple genes, including UNC13B, RAB3A, PTPRD, and SYT1.Conclusion: PPFIA1 may be associated with ESCC progression and could be used as a biomarker for prognostic evaluation in ESCC patients.
Perinatal and neonatal outcomes of pregestational diabetes mellitus: a retrospective study
Authors: Capobianco Giampiero , Gulotta Alessandra , Tupponi Giulio , Dessole Francesco , Virdis Giuseppe , Cherchi Claudio , Vita Davide , Petrillo Marco , Olzai Giorgio , Antonucci Roberto , Saderi Laura , Cherchi Pier Luigi , Dessole Salvatore , Sotgiu Giovanni 
Published: May 2021

Abstract
BackgroundThe aim of the study was to evaluate maternal-foetal and neonatal clinical outcomes in a group of patients with pregestational diabetes mellitus (PGDM) such as diabetes mellitus type 1 (DM1), diabetes mellitus type 2 (DM2), and maturity onset diabetes of the young (MODY).MethodsOverall, 174 pregnant women, nulliparous and multiparous, with single pregnancy were enrolled. The selected patients were divided into two groups: PGDM (42 with DM1, 14 with DM2, and 2 with MODY); 116 patients with a negative pathological history of diabetes mellitus were the control.ResultsWe reported an incidence of preterm delivery of 55.2% in the PGDM group, of 59.5% in the DM1 group, and 42.9% in the DM2 group VS. 6% in the controls. Foetal growth disorders, such as intrauterine growth retardation, small for gestational age, and foetal macrosomia were found in 19% and 3.6% in the case and in the control group, respectively. A relationship between DM2 and gestational hypertension was found.Conclusionspatients with PGDM are at increased risk of perinatal and neonatal complications in comparison with pregnant women without PGDM.
Determination of the velocity of an opaque body moving uniformly by measurements inside it
Authors: Elektronov Nasko 
Published: May 2021

Abstract
In this paper we show how the velocity of a moving uniform opaque body can be calculated without external references. This is done with the help of photodetectors, which measure the time of arrival of light from a point source to fixed equal distances inside the body. The calculation of the body’s velocity is based on the postulates of the special theory of relativity that space is homogeneous, isotropic and the speed of light in vacuum is an invariant constant with a certain value independent of the velocity of the radiation source.
Distribution of Desert Locust Schistocerca gregaria in Rajasthan, India and Establishing an Early Warning System for Locust Control in India
Authors: Jhiknaria Hameer 
Published: May 2021

Abstract
Desert Locust is considered to be the most serious pests that cause a devastated damage to the crops and the other agricultural products during their invasions. The Desert Locust is a major threat for food security, livelihoods, environment and economic development in a region. The recent Locust Outbreak caused major damage to standing crops and vegetables in the Central and Western states of India, including Rajasthan, Punjab, Haryana, and Madhya Pradesh, with Rajasthan being the most affected. India had experienced such massive locust invasion after two decades. Establishing an Early Warning System for Locust Control in India is essential to reduce the impact by providing timely and relevant information in a systematic way contributing to increasing in resilience of the country. The distribution of Desert Locusts in Rajasthan, India has been presented from June 2019 to August 2020, along with the key Environmental Factors of Temperature, Rainfall, Soil Moisture and Prevalence of Vegetation significantly affecting Locust Activity. All the datasets used were obtained from Secondary sources. These datasets were obtained from Open Government Data (OGD) Platform. The Maps created in the study show the Distribution of Desert Locusts in Rajasthan, India; along with this the Choropleth map show Average- Temperature, Rainfall, Soil Moisture and Normalized Difference Vegetation Index (NDVI), all at District level. The Early Warning System for Desert Locust Control in India is a key integration of four key elements of: Risk Knowledge, Monitoring and Warning Service, Dissemination and Communication and Response Capability, and four-four sub elements of each key element. Establishing an Early Warning System for Locust Control in India is of paramount importance and a major achievement for the nation itself.
Outcomes of surgical reconstruction of the superior vena cava syndrome in patients with locally advanced thymomas
Authors: Boyko Valeriy , Krasnoyaruzhskyi Andriy , Minukhin Dmytro , Dubovyk Dmytro , Ponomarova Kateryna , Sochnieva Anastasiya , Kritsak Vasil 
Published: May 2021

The aim of the study: to study and demonstrate the potential and technical aspects of the surgical treatment of locally advanced thymus tumours with the tumour infiltrate affecting superior vena cava and its branches.
Methods. 56 patients with locally advanced thymomas complicated by SVCS were study. The control group included 30 patients with SVCS were treated with conventional techniques, while the experimental group included 26 patients who underwent a novel bypass surgery developed by us.
Results. Based on our observations, patients tolerated these surgeries much better. The venous bypass was mandatorily complemented with cytoreduction. Auriculo-jugular (left and right) and auriculo-subclavian (left and right) bypasses were used in our observations
Complications in the post-operative period were reported from the experimental group and included auriculo-subclavian bypass thrombosis, post-operative complications were reported in the control group including haemorrhage from the sternotomy wound in 1 (3.3 %) case, superior vena cava thrombosis in 2 (6.6 %) cases, pneumonia in 2 (6.6 %) cases and thromboembolism of small pulmonary arteries in 2 (6.6 %) cases.
Post-operative lethality in the study groups was reported in the control and in the experimental group. Total lethality rate was 8.9 % (5 patients). The relative risk of complications and lethal outcome was calculated for patients from both groups. It was found that the risk of complications was twice as high in the control group as in the experimental group (standard error of relative risk equals 0.64), whereas the risk of lethal outcome increased by a factor of 3.5 in the control group (standard error of relative risk equals 1.09)
Conclusion. It has been established that the superior vena cava syndrome in patients with locally advanced thymoma is an emergency condition whose surgical correction must be personalised depending on the anatomic and topographic classification of SVC lesion types. It is known that an obligatory pre-condition of the perioperative period in this category of patients is an adequate vascular approach to the superior vena cava system. The first mandatory step of the radical surgery in patients with locally advanced thymomas with SVC invasion should be the auriculo-jugular and auriculo-subclavian bypasses, which can reduce the relative risk of post-operative complications by a factor of the risk of lethal by a factor of 3.5.
Peculiarities of intrauterine pathological processes in women of the postmenopauseal period
Authors: Polishchuk Tetiana , Vdovichenko Serhii , Lubkovska Oleksandra , Ledin Dmytro 
Published: May 2021

Aim of the research is studying the pecularities of the endometrium pathological processes in postmenopausal women on the basis of the hysteroscopy data evaluation, as well as conduction of histopathological and immunochemical studies.
Materials and methods. To study the pecularities of intrauterine pathological processes, 100 postmenopausal women were selected and studied. All women underwent hysteroscopy with separate diagnostic scraping of the uterine cavity. Also, 10 samples of glandular-fibrous endometrial polyps (GFEP) in women of reproductive age and 9 samples of such pathology in the postmenopausal period were studied by immunohistochemical method, using 6 primary specific monoclonal antibodies.
Results. The results showed that in 64 (64 %) postmenopausal women pathological changes of the endometrium were presented with GFEP, and in 2 patients (2 %) endometrial adenocarcinoma was found. It was also determined that the nuclei of epithelial and stromal cells of GFEP in postmenopausal women are characterized by lower expression of estrogen and progesterone receptors, compared with women with such formations in the reproductive age. In addition, it was found that in postmenopausal women the expression of the apoptosis inhibitor bcl-2 and aromatase P450 in the epithelial and stromal cells of GFEP was more expressed, and the expression of the Bах antigen, on the contrary, was significantly lower than in women of reproductive age.
Conclusions. Fundamental molecular-biological differences of GFEP in postmenopausal women compared with women of reproductive age were revealed. It was found that in postmenopausal women there is a significantly lower dependence of such polyps on the effects of estrogen and progesterone. In addition, the data indicate an increased risk of neoplastic transformation in such women.
ABO and Rh(D) blood typing on the PK 7200 with ready-to-use kits
Authors: Moncharmont P. , Plantier A. , Chirat V. , Rigal D. 
Published: May 2021

Abstract
The performance of ready-to-use kits was evaluated on the PK 7200 blood grouping system. The Olymp Group (kit 1) and Olymp Group II (kit 2) containing anti-A, -B, -AB, and -D reagents were tested for first and second determinations of A, B, and D antigens. More than 500 RBC samples, including several variant ABO and D phenotypes, were evaluated for specificity, repeatability, reproducibility, and sensitivity. Specificity was tested with well-characterized reagent RBCs. Repeatability was established by at least 12 assays per run with three reagent RBCs, and reproducibility was established on one run per day for 5 days. No discrepancy was observed in ABO and D determinations with either kit. In repeatability, three discrepancies were found with group A and B RBCs with kit 1. In reproducibility, no discrepancies were observed. The kit 1 anti-A reagent detected A3 but not Ax RBCs and anti-AB detected both. A B3 RBC was detected by both kits. Among eight weak D phenotypes, six were positive with kit 1. With kit 2, only one of five weak D phenotypes was detected.
Immunohematology 2003;19:54–56.
Assessment of the relative number of copies of the gene encoding human neutrophil antigen-2a (HNA-2a), CD177, and a homologous pseudogene by quantitative real-time PCR
Authors: Dittmar K. , Lim J-B. , Caruccio L. , Bettinotti M. , Stroncek D. 
Published: May 2021

Abstract
Human neutrophil antigen-2a (HNA-2a; NB1) is located on the 58–64 kD NB1 glycoprotein (GP) and is encoded by the gene CD177. Searches of human genome databases have revealed that a pseudogene highly homologous to exons 4–9 of CD177 is located adjacent to CD177 on chromosome 19. The purpose of this study was to document the presence of the pseudogene and determine whether the polymorphic expression of NB1 GP is due to CD177 gene deletions and duplications. Genomic DNA was isolated from leukocytes of 12 subjects. The number of copies of exon 2 of CD177, an exon that is unique to this gene, and the number of copies of exon 9, an exon that is found in both CD177 and the pseudogene, was assessed with quantitative real-time PCR. The ratio of the number of copies of sequences homologous to CD177 exon 9 to the number of copies of exon 2 was 1.5 or greater in 7 of the 12 subjects, suggesting that both CD177 and the homologous pseudogene were present. The ratio of exon 9 to exon 2 in the other 5 subjects ranged from 1 to 1.25, suggesting that the pseudogene was not present in these subjects. However, results of assays were variable and we could not exclude the possibility that all subjects carried the pseudogene. These studies confirmed the presence of the pseudogene homologous to CD177, but quantitative real-time PCR was not precise enough to detect CD177 duplications or deletions.
Anti-Mta associated with three cases of hemolytic disease of the newborn
Authors: Cheung C.C. , Challis D. , Fisher G. , Russell S.J. , Davis A. , Bruce H. , Watt J. , Chong B.H. 
Published: May 2021

Abstract
The Mta antigen is a low-frequency red blood cell (RBC) surface antigen and is an established antigen of the MNSs blood group system. There has been one report of anti-Mta–induced hemolytic disease of the newborn (HDN) in the literature to date. We describe a family in which three children were affected by neonatal anemia. The clinical and hematologic findings were consistent with HDN, despite repeatedly negative direct antiglobulin tests (DAT) on cord RBCs. Serologic investigations showed that the mother’s serum contained anti-Mta. The father and all three children phenotyped as Mta+, while the mother was Mta–. Adsorption and elution experiments gave results which suggested that anti-Mta may be implicated in recurrent HDN in this family.
Immunohematology 2002;18:37–39.
Screening for RBC antibodies— what should we expect from antibody detection RBCs
Authors: Garratty G. 
Published: May 2021

Abstract
In the United States, the Food and Drug Administration mandates that red blood cells (RBCs) for antibody detection possess the following antigens: C, D, E, c, e, M, N, S, s, P1,Lea,Leb,K,k,Fya,Fyb,Jka, and Jkb. Although not required, it is generally agreed that homozygosity for C,D,E,c,e,Fya,and Jka is also preferable.There is no requirement for low-frequency antigens to be present.However, manufacturers of antibody detection RBCs receive requests for these RBCs to possess Cw,Kpa,and Wra.There are no data to support the considerable expense and effort involved in providing RBCs that possess low-frequency antigens such as Cw, Kpa, and Wra. The risk of clinically-significant hemolytic transfusion reactions occurring when such antibodies are not detected because antibody detection RBCs lack such antigens is about 1 in 500,000 to 1 in 1 million transfusions.
Immunohematology 2002;18:71–77.
Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
Authors: Ranimol G. , Paul Chinju , Sunkar Swetha 
Published: May 2021

Abstract
Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzyme immobilized onto zein–polyvinyl pyrrolidone (PVP) composite nanofiber is done. The nanofibers were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) analysis. pH and temperature profiles of immobilized enzyme were found to be broader than its free counterpart. The Km value was found to be 0.243 mM for free laccase and 0.958 mM for immobilized laccase. Similarly, Vmax for the free enzyme was 3.572 U/mg compared to 2.48 U/mg of immobilized laccase. The relative activity of immobilized laccase was 64.91% after storage for 30 days at room temperature while it was 28.64% for free laccase. The temperature and pH for AR 1 decolorization were optimized and was found to be 60 °C and 5, respectively. Also, decolorization percentage was found to be 91.67% for immobilized laccase and 72.03% of free laccase in the presence of natural mediators like vanillin. From phytotoxicity studies it was found that the germination rate, shoot and root length was increased compared to untreated dye. Therefore, zein–PVP nanofiber immobilized laccase could be an ideal candidate for the textile dye decolorization.
Sequestration of p-nitrophenol from liquid phase by poly(acrylonitrile-co-acrylic acid) containing thioamide group
Authors: Soo J. W. , Abdullah L. C. , Jamil S. N. A. M. , Adeyi A. A. 
Published: May 2021

Abstract
In this paper, the adsorptive performance of synthesized thiourea (TU) modified poly(acrylonitrile-co-acrylic acid) (TU-P(AN-co-AA)) polymeric adsorbent for capturing p-nitrophenol (PNP) from aqueous solution was investigated. TU-P(AN-co-AA) was synthesized via the redox polymerization method with acrylonitrile (AN) and acrylic acid (AA) as the monomers, then modified chemically with thiourea (TU). Characterization analysis with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), elemental microanalysis for CHNS, zeta potential measurement, Brunauer–Emmett–Teller (BET) surface analysis and thermal analyses were carried out to determine the morphology and physico-chemical properties of the synthesized polymer. The characterization results indicated successful surface modification of polymer with TU. The performance of TU-P(AN-co-AA) for the removal of PNP was investigated under various experimental parameters (adsorbent dosage, initial adsorbate concentration, contact time and temperature). The results demonstrated that the Freundlich isotherm model and pseudo-second-order kinetic model best described the equilibrium and kinetic data, respectively. Thermodynamic studies showed that the uptake of PNP by TU-P(AN-co-AA) was spontaneous and exothermic in nature. The results of the regeneration studies suggested that the TU-P(AN-co-AA) polymer is a reusable adsorbent with great potential for removing PNP from wastewater.
Biomarkers and Tools for Predicting Alzheimer’s Disease in the Preclinical
Stage
Authors: Li Tao-Ran , Yang Qin , Hu Xiaochen , Han Ying 
Published: May 2021

:
Alzheimer’s disease (AD) is the only leading cause of death for which no disease-modifying
therapy is currently available. Over the past decade, a string of disappointing clinical trial results has
forced us to shift our focus to the preclinical stage of AD, which represents the most promising therapeutic
window. However, the accurate diagnosis of preclinical AD requires the presence of brain β-
amyloid deposition determined by cerebrospinal fluid or amyloid-positron emission tomography, significantly
limiting routine screening and diagnosis in non-tertiary hospital settings. Thus, an easily
accessible marker or tool with high sensitivity and specificity is highly needed. Recently, it has been
discovered that individuals in the late stage of preclinical AD may not be truly “asymptomatic” in that
they may have already developed subtle or subjective cognitive decline. In addition, advances in bloodderived
biomarker studies have also allowed the detection of pathologic changes in preclinical AD.
Exosomes, as cell-to-cell communication messengers, can reflect the functional changes of their source
cell. Methodological advances have made it possible to extract brain-derived exosomes from peripheral
blood, making exosomes an emerging biomarker carrier and liquid biopsy tool for preclinical AD. The
eye and its associated structures have rich sensory-motor innervation. In this regard, studies have indicated
that they may also provide reliable markers. Here, our report covers the current state of
knowledge of neuropsychological and eye tests as screening tools for preclinical AD and assesses the
value of blood and brain-derived exosomes as carriers of biomarkers in conjunction with the current
diagnostic paradigm.
Whether Erythropoietin can be a Neuroprotective Agent against Premature
Brain Injury: Cellular Mechanisms and Clinical Efficacy
Authors: Shi Yuan , Ma Xueling 
Published: May 2021

:
Preterm infants are at high risk of brain injury. With more understanding of the preterm
brain injury's pathogenesis, neuroscientists are looking for more effective methods to prevent and
treat it, among which erythropoietin (Epo) is considered as a prime candidate. This review tries to
clarify the possible mechanisms of Epo in preterm neuroprotection and summarize updated evidence
considering Epo as a pharmacological neuroprotective strategy in animal models and clinical
trials. To date, various animal models have validated that Epo is an anti-apoptotic, antiinflammatory,
anti-oxidant, anti-excitotoxic, neurogenetic, erythropoietic, angiogenetic, and neurotrophic
agent, thus preventing preterm brain injury. However, although the scientific rationale and
preclinical data for Epo's neuroprotective effect are promising, when translated to bedside, the results
vary in different studies, especially in its long-term efficacy. Based on existing evidence, it is
still too early to recommend Epo as the standard treatment for preterm brain injury.
sFRP1 Expression Regulates Wnt Signaling in Chronic Myeloid Leukemia K562 Cells
Authors: Pehlivan Melek , Caliskan Ceyda , Yuce Zeynep , Sercan Hakki O. 
Published: May 2021

Background:
Wnt signaling cascades play important roles in cell fate decisions and their deregulation
has been documented in many diseases, including malignant tumors and leukemia. One mechanism of aberrant
Wnt signaling is the silencing of Wnt inhibitors through epigenetic mechanisms. The sFRPs are one of the most
studied Wnt inhibitors; and the sFRP1 loss is known in many hematological malignancies. Therefore, we aimed
to compare the expression of Wnt related genes in the presence and absence of sFRP1 in a chronic myeloid
leukemia (CML) cell line.

Objective:
It is important to understand how sFRP1 and sFRP1 perform their effects on CML to design new
agents and strategies for resistant and advanced forms of CML.

Materials and Methods:
We used K562 cells, which normally do not express sFRP1 and its sFRP1 expressing
subclone K562s. Total RNA was isolated from K562 and K562s cell lines and converted to cDNA. PCR Array
experiments were performed using Human Wnt Signaling Pathway Plus RT2 Profiler™ kit. Wnt signaling pathway
activation was studied by western blot for downstream signaling targets.

Results:
The WNT3, LRP6, PRICKLE1 and BTRC expressions were significantly decreased in the presence of
sFRP1; while WNT5B increased. The sFRP1 expression inhibited stabilization of total β-catenin protein and
downstream effector phosphorylation of noncanonical Wnt/PCP signaling; whereas Ca2+/PKC signaling remained
active.

Conclusion:
The results suggest that sFRP1 could be a promising therapeutic anticancer agent. Defining these
pathway interactions is crucial for designing new agents resistant and advanced forms of CML.