bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing carnivore spatial co‐occurrence and temporal overlap in the face of human interference in a semiarid forest
Authors: Zanón Martínez Juan I. , Seoane Javier , Kelly Marcella J. , Sarasola José Hernán , Travaini Alejandro 
Published: October 2021

AbstractApex predators drive top‐down effects in ecosystems and the loss of such species can trigger mesopredator release. This ecological process has been well documented in human‐modified small areas, but for management and conservation of ecological communities, it is important to know which human factors affect apex predator occurrence and which mediate mesopredators release at large scales. We hypothesized that mesopredators would avoid spatial and temporal overlap with the apex predator, the puma; but that human perturbations (i.e., cattle raising and trophy hunting) would dampen top‐down effects and mediate habitat use. We installed 16 camera traps in each of 45, 10 × 10 km grid cells in the Caldén forest region of central Argentina resulting in 706 total stations covering 61,611 km2. We used single‐season occupancy and two‐species co‐occurrence models and calculated the species interaction factor (SIF) to explore the contributions of habitat, biotic, and anthropic variables in explaining co‐occurrence between carnivore pairs. We also used kernel density estimation techniques to analyze temporal overlap in activity patterns of the carnivore guild. We found that puma habitat use increased with abundance of large prey and with proximity to protected areas. Geoffroy's cats and skunks spatially avoided pumas and this effect was strong and mediated by distance to protected areas and game reserves, but pumas did not influence pampas fox and pampas cat space use. At medium and low levels of puma occupancy, we found evidence of spatial avoidance between three pairs of mesocarnivores. All predators were mostly nocturnal and crepuscular across seasons and mesopredators showed little consistent evidence of changing activity patterns with varying levels of puma occupancy or human interference. We found potential for mesopredator release at large scale, especially on the spatial niche axis. Our results suggest that a combination of interacting factors, in conjunction with habitat features and intervening human activities, may make mesopredator release unlikely or difficult to discern at broad scales. Overall, we believe that promoting the creation of new protected areas linked by small forest patches would likely lead to increased predator and prey abundances, as well as the interactions among carnivores inside and outside of protected areas.
Finding the right mix: a framework for selecting seeding rates for cover crop mixtures
Authors: Bybee‐Finley K. Ann , Cordeau Stéphane , Yvoz Séverin , Mirsky Steven B. , Ryan Matthew R. 
Published: October 2021

AbstractCover crop mixtures have the potential to provide more ecosystem services than cover crop monocultures. However, seeding rates that are typically recommended (i.e. seeding rate of monoculture divided by the number of species in the mixture) are non‐optimized and often result in the competitive species dominating the mixture, and therefore limiting the amount of ecosystem services that are provided. We created an analytical framework for selecting seeding rates for cover crop mixtures that maximize multifunctionality while minimizing seed costs. The framework was developed using data from a field experiment, which included six response surface designs of two‐species mixtures, as well as a factorial replacement design of three‐species and four‐species mixtures. We quantified intraspecific and interspecific competition among two grasses and two legume cover crop species with grass and legume representing two functional groups: pearl millet [Pennisetum glaucum (L.) R.Br.], sorghum sudangrass [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf], sunn hemp (Crotalaria juncea L.), and cowpea [Vigna unguiculata (L.) Walp]. Yield–density models were fit to estimate intraspecific and interspecific competition coefficients for each species in biculture. The hierarchy from most to least competitive was sorghum sudangrass > sunn hemp > pearl millet > cowpea. Intraspecific competition of a less competitive species was the greatest when the biculture was composed of two species in the same functional group. Competition coefficients were used to build models that estimated the biomass of each cover crop species in three‐species and four‐species mixtures. The competition coefficients and models were validated with an additional nine site‐years testing the same cover crop mixtures. The biomass of a species in a site‐year was accurately predicted 69% of the time (low root mean square error, correlation > 0.5, not biased, r2 > 0.5). Applying the framework, we designed three‐species and four‐species mixtures by identifying relative seeding rates that produced high biomass with high species evenness (i.e. high multifunctionality) at low seed costs based on a Pareto front analysis of 10,418 mixtures. Accounting for competition when constructing cover crop mixtures can improve the ecosystem services provided, and such an advancement is likely to lead to greater farmer adoption.
Factor structure and psychometric properties of the Muscularity‐Oriented Eating Test in university women in Australia
Authors: Cunningham Mitchell L. , Rodgers Rachel F. , Pinkus Rebecca T. , Nagata Jason M. , Trompeter Nora , Mitchison Deborah , Murray Stuart B. , Szabo Marianna , Lavender Jason M. 
Published: October 2021

AbstractObjectiveToned muscularity continues to emerge as a salient aspect of women's body image. However, there is a dearth of research investigating the potentially maladaptive eating practices and related cognitions that accompany the drive for muscularity in women. This may be attributable to the limited empirical and clinical attention previously given to muscularity‐oriented disordered eating and, accordingly, the lack of validated measures assessing these concerns. To address this knowledge gap, our study aimed to provide a preliminary evaluation of the factor structure and core psychometric properties of a recently developed measure of muscularity‐oriented disordered eating, the Muscularity‐Oriented Eating Test (MOET), in university women in Australia.MethodParticipants included 419 university women who completed the 15‐item MOET and other self‐report measures for validity evaluation as part of an online survey. Data from split‐half samples were used to undertake an exploratory factor analysis and subsequent confirmatory factor analysis.ResultsFactor analytic results supported a briefer (12‐item), one‐factor scale in this sample of university women. The internal consistency reliability and validity (convergent and discriminant) of this 12‐item unidimensional MOET was supported.DiscussionOur study provides preliminary support for this modified MOET as a psychometrically sound self‐report measure of muscularity‐oriented disordered eating in university women in Australia, providing a useful tool for understanding maladaptive eating behaviors and cognitions concomitant to the pursuit of muscularity in this population.
Parallel morphological evolution and habitat‐dependent sexual dimorphism in cave‐ vs. surface populations of the Asellus aquaticus (Crustacea: Isopoda: Asellidae) species complex
Authors: Balázs Gergely , Biró Anna , Fišer Žiga , Fišer Cene , Herczeg Gábor 
Published: October 2021

AbstractStudying parallel evolution (repeated, independent evolution of similar phenotypes in similar environments) is a powerful tool to understand environment‐dependent selective forces. Surface‐dwelling species that repeatedly and independently colonized caves provide unique models for such studies. The primarily surface‐dwelling Asellus aquaticus species complex is a good candidate to carry out such research, because it colonized several caves in Europe. By comparing 17 functional morphological traits between six cave and nine surface populations of the A. aquaticus species complex, we investigated population divergence in morphology and sexual dimorphism. We found habitat‐dependent population divergence in 10 out of 17 traits, likely reflecting habitat‐driven changes in selection acting on sensory systems, feeding, grooming, and antipredator mechanisms. Sexual dimorphism was present in 15 traits, explained by sexual selection acting on male traits important in male–male agonistic behavior or mate guarding and fecundity selection acting on female traits affecting offspring number and nursing. In eight traits, the degree of sexual dimorphism was habitat dependent. We conclude that cave‐related morphological changes are highly trait‐ and function‐specific and that the strength of sexual/fecundity selection strongly differs between cave and surface habitats. The considerable population variation within habitat type warrants further studies to reveal cave‐specific adaptations besides the parallel patterns.
Basic verification of cancer cell separation characteristics in a dielectrophoretic device using microcylindrical electrodes
Authors: Miyamukai Hideo , Yagi Ippei , Uchida Satoshi , Takano Masayo , Wakizaka Yoshikazu , Enjoji Takaharu 
Published: October 2021

AbstractHighly accurate isolation of circulating tumor cells (CTC) in the blood is essential for early detection of cancer. In particular, the dielectrophoretic device with a three‐dimensional (3D) structure can be expected to have separation accuracy, which cannot be obtained with conventional 2D shapes. In this study, an isolating leukemia‐derived suspended Jurkat cell and fluorescent polystyrene particle, which are particles of uniform size, using a 3D microcylindrical electrodes are conducted. When the concentration of Jurkat cells was reduced to 1/100 of the concentration of polystyrene particles, Jurkat cells and polystyrene particles could be efficiently separated, and the collection efficiency at Outlet II, the outlet for the collection of target cells, was 100% and 2%, respectively. It was suggested that the proposed device may be able to separate cells of similar size with high accuracy.
ARNI versus ACEI/ARB in Reducing Cardiovascular Outcomes after Myocardial Infarction
Authors: She Jianqing , Lou Bowen , Liu Hui , Zhou Bo , Jiang Gulinigaer Tuerhong , Luo Yongbai , Wu Haoyu , Wang Chen , Yuan Zuyi 
Published: October 2021

AbstractAimsThis study aimed to compare the efficacy of angiotensin receptor‐neprilysin inhibitor (ARNI) therapy with angiotensin converting enzyme inhibitor or angiotensin receptor blocker (ACEI/ARB) therapy for cardiovascular outcomes in patients with acute myocardial infarction (AMI).Methods and resultsData were collected from the Biobank of the First Affiliated Hospital of Xi'an Jiaotong University between January 2016 and December 2020. A total of 7556 AMI patients were screened for eligibility. Propensity score matching based on age, sex, blood pressure, kidney function, baseline left ventricular ejection fraction (LVEF), and cardiovascular medication were conducted, resulting in 291 patients with AMI being assigned to ARNI, ACEI, and ARB group, respectively. Patients receiving ARNI had significantly lower rates of the composite cardiovascular outcome than ACEI {hazard ratio [HR] 0.51, [95% confidence interval (CI), 0.27–0.95], P = 0.02}, and ARB users [HR 0.47, (95%CI, 0.24–0.90), P = 0.02]. Patients receiving ARNI showed lower rates of cardiovascular death than ACEI [HR 0.37, (95%CI, 0.18–0.79), P = 0.01] and ARB users [HR 0.41, (95%CI, 0.18–0.95), P = 0.04]. Subgroup analysis indicated that patients with LVEF no more than 40% tend to benefit more from ARNI as compared with ACEI [HR 0.30, (95%CI, 0.11–0.86), P = 0.01] or ARB [HR 0.21, (95%CI, 0.04–1.1), P = 0.05]. Patients aged no more than 60 years exhibited reduced composite endpoints [HR for ARNI vs. ARB: 0.11, (95%CI, 0.03–0.46), P = 0.002].ConclusionsIn patients with AMI, ARNI was superior to ACEI/ARB in reducing the long‐term adverse cardiovascular outcomes. Subgroup analysis further indicates that ARNI is more likely to benefit patients with LVEF less than 40% and aged less than 60 years.
Controlling Selectivity in the Synthesis of Z‐α,β‐Unsaturated Amidines by Tuning the N‐Sulfonyl Group in a Rhodium(II) Catalyzed 1,2‐H Shift
Authors: Martin Matthew L. , Boyer Alistair 
Published: October 2021

AbstractN2‐Sulfonyl‐α‐diazo amidines can be synthesized by the reaction of electron rich alkynyl amines with electron poor sulfonyl azides through 1,3‐dipolar cycloaddition that proceeds with perfect regioselectivity. In the presence of rhodium(II) carboxylate catalysts, denitrogenation occurs to give the corresponding metallocarbene but there are then two competing processes: 1,2–H shift and O‐transfer from the sulfonyl group to the metallocarbene center. The outcome can be controlled using an electron poor nitrobenzenesulfonyl group and large carboxylate rhodium ligands to select for 1,2–H shift, forming α,β‐unsaturated amidines in high yield and with excellent Z‐selectivity.
A highly selective tri‐band bandpass‐to‐bandstop filter with the third band independently tunable
Authors: Yang Hong , Zhang Dewei , Zhang Junjie , Wang Xian , Lv Dalong , Zhang Yi , Zhou Dongfang 
Published: October 2021

AbstractA highly selective tri‐band bandpass (BP)‐to‐bandstop (BS) filter with the third tunable band and the other two fixed ones is proposed in this article. This filter is based on a novel crossed resonator that is composed of a two‐end‐open UIR with an open stub and a short stub connected in parallel on both sides of the resonator center. A PIN diode is utilized to achieve BP‐to‐BS switchable characteristics. Then, varactor diodes loaded at the open stub are employed to obtain the independently tunable third band. In addition, due to the symmetry of the resonator structure, the even‐ and odd‐ mode analysis method is used to derive the resonant frequencies. For validation, a tri‐band BP‐to‐BS filter operating at 2.4 GHz (WLAN), 3.3 GHz (WiMAX), and 4.9 GHz (LAA/LTE‐U) is designed, manufactured, and measured that achieves the continuous frequency tuning range (FTR) of the third band from 4.52 to 4.90 GHz with return loss (RL) better than 10.05 dB under the BP characteristic, from 4.55 to 4.90 GHz with the rejection level (RL) higher than 15.53 dB under the BS characteristic. The measured results agree well with the simulated ones.
Changes in Sewage Sludge Chemical Signatures During a COVID-19 Community Lockdown, Part 1: Traffic, Drugs, Mental Health, and Disinfectants
Authors: Nason Sara L. , Lin Elizabeth , Eitzer Brian , Koelmel Jeremy , Peccia Jordan 
Published: October 2021

Abstract
The early months of the COVID-19 pandemic and the associated shutdowns disrupted many aspects of daily life and thus caused changes in the use and disposal of many types of chemicals. While records of sales, prescriptions, drug overdoses, and so forth provide data about specific chemical uses during this time, wastewater and sewage sludge analysis can provide a more comprehensive overview of chemical changes within a region. We analyzed primary sludge from a wastewater-treatment plant in Connecticut, USA, collected March 19 to June 30, 2020. This time period encompassed the first wave of the pandemic, the initial statewide stay at home order, and the first phase of reopening. We used liquid chromatography–high-resolution mass spectrometry and targeted and suspect screening strategies to identify 78 chemicals of interest, which included pharmaceuticals, illicit drugs, disinfectants, ultraviolet (UV) filters, and others. We analyzed trends over time for the identified chemicals using linear trend analyses and multivariate comparisons (p &lt; 0.05). We found trends related directly to the pandemic (e.g., hydroxychloroquine, a drug publicized for its potential to treat COVID-19, had elevated concentrations in the week following the implementation of the US Emergency Use Authorization), as well as evidence for seasonal changes in chemical use (e.g., increases for three UV-filter compounds). Though wastewater surveillance during the pandemic has largely focused on measuring severe acute respiratory syndrome–coronavirus-2 RNA concentrations, chemical analysis can also show trends that are important for revealing the public and environmental health effects of the pandemic. Environ Toxicol Chem 2022;41:1179–1192. © 2021 SETAC
Target and Suspect Screening of Urinary Biomarkers for Current-use Pesticides: Application of a Simple Extraction Method
Authors: Huang Zhoubing , Li Huizhen , Xiong Jingjing , You Jing 
Published: October 2021

Abstract
Pesticide residues pose a great threat to human health. Biomonitoring with urine samples has often been used to assess pesticide exposure to humans, and identifying appropriate biomarkers is a premise of success. Current-use pesticides (CUPs) including neonicotinoids tend to be transformed in an organism, and thus the biomonitoring studies focusing on parent compounds alone may underestimate their risk. It is imperative to develop effective methods to analyze CUPs and their metabolites simultaneously and to identify viable metabolites as urinary biomarkers. For analyzing xenobiotics in urine, we optimized CH3COCH3-MgSO4 extraction coupled with a high-performance liquid chromatography–tandem mass spectrometry detection method. The method had sensitive method detection limits (0.11–1.39 ng/ml), low matrix effects, and satisfactory recovery and precision (49.4% ± 7.2%–99.8% ± 17.8%) for neonicotinoids and their metabolites. Application of the method for real samples showed that children living in rural areas in South China were ubiquitously exposed to CUPs, including neonicotinoids, fipronil, and chlorpyrifos, and urinary residues were mainly in the form of metabolites. Suitable biomarkers were identified for individual neonicotinoids, including imidacloprid–olefin and imidacloprid–guanidine for imidacloprid, acetamiprid-N-desmethyl for acetamiprid, thiacloprid–amide for thiacloprid, and N-desmethyl-thiamethoxam and thiamethoxam for thiamethoxam. Three metabolites were mainly reported in urine samples, including imidacloprid–urea, thiacloprid–amide, and N-desmethyl-thiamethoxam. In addition, the method was also applied for suspect screening, and an additional metabolite (clothianidin–desmethyl–nitrosoguanidine) was identified, showing its potential application in suspect analysis. Environ Toxicol Chem 2022;41:73–80. © 2021 SETAC
Abstract
Urinary biomarkers for neonicotinoid biomonitoring were identified using target and suspect analyses after a simple and rapid extraction.
Rice seedlings grown under high ammonia do not show enhanced defence responses
Authors: Zhou Heng , Zhou Ying , Zhai Fengchao , Wu Ting , Xie Yanjie , Xu Guohua , Foyer Christine H. 
Published: October 2021

AbstractUrea‐based fertilizers are commonly used in the agricultural rice production. Urea produces gaseous ammonia upon contact with plants and soil. Although rice is considered to be a relatively ammonium‐tolerant crop, urea fertilization can have a negative impact on seedling establishment because of tissue ammonia accumulation. We investigated the molecular/genetic regulation of ammonia tolerance in rice using a multidisciplinary approach consisting of high‐resolution LC‐MS/MS analysis and bioinformatics, together with plant molecular physiology and biochemistry. Growth with 10 or 80 mM NH4Cl led to an inhibition of photosynthesis and ROS accumulation. Proteomic analysis revealed changes in the levels of 266 proteins, which function in translation and protein metabolism, amino acid and carbon metabolism/transport, antioxidant and redox metabolism. The abundance of the transcripts encoding these proteins was increased under high ammonium. Data are presented showing that ammonia‐induced changes in metabolism are linked to a strong antioxidant response but little change in secondary metabolism.
Targeting SARS‐CoV‐2 with Chaga mushroom: An in silico study toward developing a natural antiviral compound
Authors: Eid Jehane Ibrahim , Das Biswadeep , Al‐Tuwaijri Majdah Mohamed , Basal Wesam Taha 
Published: October 2021

AbstractThe novel coronavirus (SARS‐CoV‐2) has caused large‐scale global outbreaks and mainly mediates host cell entry through the interaction of its spike (S) protein with the human angiotensin‐converting enzyme‐2 (ACE‐2) receptor. As there is no effective treatment for SARS‐CoV‐2 to date, it is imperative to explore the efficacy of new compounds that possess potential antiviral activity. In this study, we assessed the potential binding interaction of the beneficial components of Chaga mushroom, a natural anti‐inflammatory and immune booster with that of the SARS‐CoV‐2 receptor‐binding domain (RBD) using molecular docking, MD simulation, and phylogenetic analysis. Beta glycan, betulinic acid, and galactomannan constituents of Chaga mushroom exhibited strong binding interaction (−7.4 to −8.6 kcal/mol) forming multivalent hydrogen and non‐polar bonds with the viral S1‐carboxy‐terminal domain of the RBD. Specifically, the best interacting sites for beta glycan comprised ASN‐440, SER 373, TRP‐436, ASN‐343, and ARG 509 with average binding energy of −8.4 kcal/mol. The best interacting sites of galactomannan included ASN‐437, SER 373, TRP‐436, ASN‐343, and ALA 344 with a mean binding energy of −7.4 kcal/mol; and the best interacting sites of betulinic acid were ASN‐437, SER 373, TRP‐436, PHE 342, ARG 509, and ALA 344 that strongly interacted with the S‐protein (ΔG = −8.1 kcal/mol). The docking results were also compared with an S‐protein binding analog, NAG and depicted similar binding affinities compared with that of the ligands (−8.67 kcal/mol). In addition, phylogenetic analysis using global isolates depicted that the current SARS‐CoV‐2 isolates possessed a furin cleavage site (NSPRRA) in the RBD, which was absent in the previous isolates that indicated increased efficacy of the present virus for enhanced infection through increased interaction with ACE‐2. The results showed that Chaga could be an effective natural antiviral that can supplement the current anti‐SARS‐CoV‐2 drugs.
Macronutrient content and fatty acid composition and their positional distribution in human breast milk from Zhejiang Province, China in different lactation periods
Authors: He Guanghua , Li Guipu , Jiang Yanxi , Hua Jiacai , Chu Xiaojun , Xiong Lina , Gong Jinyan , Xiao Gongnian , Ye Xingqian 
Published: October 2021

AbstractLactational changes in macronutrient content, lipid profile, fatty acid composition, and positional distribution of human breast milk were investigated in this study. A total of 378 milk samples of six different lactation periods, including 0‒5, 6‒14, 15‒30, 31‒90, 91‒180, and 181‒360 days, were collected cross‐sectionally from healthy lactating women in Zhejiang, China. As lactation progressed from 0‒5 to 15‒30 days, the lipid content and the percentages of C10:0, C12:0, C14:0, C18:2n‐6, and C18:3n‐3 increased significantly, while the protein concentration and the proportions of phospholipids, cholesterols, C16:0, C18:1n‐9, C24:1n‐9, C20:4n‐6, C22:4n‐6, C22:5n‐3, and C22:6n‐3 decreased notably. When lactation was further extended to 181‒360 days, the protein content continued to decrease, and the percentages of C12:0 and C14:0 continued to increase, whereas the levels of other tested nutrients remained stable. Although the triacylglycerol positional distributions of some fatty acids underwent significant lactational variations, C14:0, C16:0, C24:1n‐9, C22:4n‐6, C22:5n‐3, and C22:6n‐3 were located mainly at the sn‐2 position, while C18:1n‐9, C18:2n‐6, and C18:3n‐3 were primarily distributed at the sn‐1,3 positions. Compared with human breast milk reported in Western countries, samples in our study demonstrated higher percentages of C18:2n‐6, C18:3n‐3, C20:4n‐6, and C22:6n‐3, but lower proportions of C12:0, C14:0, and C18:1n‐9. The results from this study indicated a nutritional composition different from that of the Western countries and may provide useful data for the development of infant formulas closer to Chinese breast milk in terms of the fatty acid composition and its specified positional distribution on triglyceride structure.