bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isoniazid‐Sugar–Triazole‐Based Fluorescent “Turn‐On” Chemosensor for Zn2+ and Mn2+ Detection With Potential Biological Applications
Authors: Bhavya P. V. , Malecki Jan Grzegorz , Das T. Mohan 
Published: February 2025

AbstractA multifunctional isoniazid‐sugar–triazole based Schiff‐bases were designed and synthesized, and their sensing capability was studied with various metal ions by colorimetric, absorption, and spectrofluorometric techniques. Interestingly, the molecule processing diethylamine moiety (11) shows fluorescent “turn‐on” chemosensor properties in the presence of Zn2+ and Mn2+. The limit of detection (LOD) values of the chemosensor toward the respective metal ions were 3.37 µM and 1.46 µM, with a 1:2 stoichiometric ratio, respectively. Compound 11 was successfully applied to effectively detect Zn2⁺ and Mn2⁺ in real water samples. Additionally, a logic gate was constructed, where the fluorescence intensity served as the output signal, responding to the chemical species as the inputs. Synthesized compounds were screened for their antibacterial activities against a panel of pathogenic bacteria, such as Staphylococcus aureus, Streptococcus pyogenes, and E. coli. All the compounds showed significant activities against these pathogens. The anticancer activity of compound 13 was investigated in human osteosarcoma (HOS) cells. MTT assay results revealed that the hybrid sugar derivative exhibited cytotoxicity against the HOS cell line, with increased potency observed at a concentration of 60 µg/ml.
A Fibronectin (FN)‐Silk 3D Cell Culture Model as a Screening Tool for Repurposed Antifibrotic Drug Candidates for Endometriosis
Authors: Teworte Sarah , Behrens Mark C. , Widhe Mona , Gurzeler Lukas‐Adrian , Hedhammar My , Luciani Paola 
Published: February 2025

AbstractThis study advances sustainable pharmaceutical research for endometriosis by developing in vitro 3D cell culture models of endometriotic pathophysiology that allow antifibrotic drug candidates to be tested. Fibrosis is a key aspect of endometriosis, yet current cell models to study it remain limited. This work aims to bridge the translational gap between in vitro fibrosis research and preclinical testing of non‐hormonal drug candidates. When grown in a 3D matrix of sustainably produced silk protein functionalized with a fibronectin‐derived cell adhesion motif (FN‐silk), endometrial stromal and epithelial cells respond to transforming growth factor beta‐1 (TGF‐β1) in a physiological manner as probed at the messenger RNA (mRNA) level. For stromal cells, this response to TGF‐β1 is not observed in spheroids, while epithelial cell spheroids behave similarly to epithelial cell FN‐silk networks. Pirfenidone, an antifibrotic drug approved for the treatment of idiopathic pulmonary fibrosis, reverses TGF‐β1‐induced upregulation of mRNA transcripts involved in fibroblast‐to‐myofibroblast transdifferentiation of endometrial stromal cells in FN‐silk networks, supporting pirfenidone's potential as a repurposed non‐hormonal endometriosis therapy. Overall, endometrial stromal cells cultured in FN‐silk networks—which are composed of a sustainably produced, fully defined FN‐silk protein—recapitulate fibrotic cellular behavior with high fidelity and enable antifibrotic drug testing.
Oxygen Modulation by Ni Metal Sites in Resorcinol‐Formaldehyde Resins for Boosted Photocatalytic H2O2 Production From Pure Water
Authors: Sun Kaiqu , Guo Lan , Luo Jun , Xiong Bo , Hou Jianhua , Lu Changyu , Du Xin , Shi Weilong 
Published: February 2025

AbstractUtilizing polymeric catalysts to generate hydrogen peroxide (H2O2) via photocatalysis for various industrial applications is a promising avenue, but the application potential is constrained by their low charge separation efficiency and the capacity for oxygen adsorption and activation. Herein, a polymeric catalyst (NiRF) modified with Ni metal sites on resorcinol‐formaldehyde (RF) resin is presented, which can efficiently produce H2O2 through photocatalysis in pure water. The optimal NiRF‐5 exhibits an impressive H2O2 generation rate of up to 31.75 mm g−1 h−1 under visible light irradiation, significantly outperforming most catalysts reported to date. Experimental results coupled with theoretical calculations simulation, indicate that the incorporation of nickel ions modulates the band structure of the NiRF resin and provides active sites conducive to the transformation of oxygen. The Ni metal active sites significantly regulate the adsorption capacity of NiRF for O2 and ensure a suitable adsorption configuration and energy. This work provides a novel design concept for RF‐based photocatalysts and offers an approach for the development of methods for the efficient photocatalytic production of H2O2.
A Flexible Framework to Ensure Traceability, Consistency, and Propagation of KPIs Evolution
Authors: Domínguez Eladio , Pérez Beatriz , Rubio Ángel L. , Zapata María A. 
Published: February 2025

ABSTRACTOrganizations use key performance indicators (KPIs) to assess the effectiveness and efficiency of their procedures and processes. In a world that is constantly evolving and hyperconnected via the internet, it is of great interest to analyze how changes (organizational, legal, technological or other) can lead to modifications in the KPIs involved. However, little attention has been paid to KPI evolution either in the scientific literature or in developed solutions. This paper presents A Flexible Framework for the Evolution, Consistency and Traceability of KPIs (AFFECTK) that aims at establishing the basis for suitable KPIs' evolution management. The feasibility of this proposal is demonstrated through a proof‐of‐concept developed using a reasoning tool based on Constraint Logic Programming. The framework is further evaluated, using real KPI case studies, to assess the functional suitability of our approach.
Surface and Interface Engineering of Electrospun Nanofibers for Heterogeneous Catalysts
Authors: Yue Guichu , Wang Dezhi , Song Feiyan , Wang Nü , Cui Zhimin , Bai Jie , Zhao Yong 
Published: February 2025

AbstractSurface and interface engineering of catalysts from atomic level to macroscale exhibit good performance in regulating conversion, selectivity, and stability. Electrospinning offers such multiscale flexibility in tuning surface and interface structures and compositions for the design of fiber catalysts. This review presents an overview on the surface and interface engineering of electrospun nanofibers for heterogeneous catalysts designing. First, the building strategies for regulating catalytic performance on surface and interface at different scales are introduced. Then, typical research achievements of surface and interface regulation strategies of nanofiber catalysts in different scales are summarized, including atomic vacancy and doping at microscale, heterojunction interfaces at mesoscale, and surfaces/interfaces with special wettability at macroscale. The typical catalytic reactions are introduced that involve classical small molecule hydrogenation, oxygen evolution reaction, and pollutant photocatalytic degradation, as well as the recently emerging CO2 reduction reaction and nitrate/nitrite reduction. Finally, the challenges and future tendency on surface and interface engineering of electrospun nanofiber catalysts are highlighted.
Optimal implementation of genomic selection in clone breeding programs exemplified in potato: II. Effect of selection strategy and cross‐selection method on long‐term genetic gain
Authors: Wu Po‐Ya , Stich Benjamin , Hartje Stefanie , Muders Katja , Prigge Vanessa , Van Inghelandt Delphine 
Published: February 2025

AbstractDifferent cross‐selection (CS) methods incorporating genomic selection (GS) have been used in diploid species to improve long‐term genetic gain and preserve diversity. However, their application to heterozygous and autotetraploid crops such as potato (Solanum tuberosum L.) is lacking so far. The objectives of our study were to (i) assess the effects of different CS methods and the incorporation of GS and genetic variability monitoring on both short‐ and long‐term genetic gains compared to strategies using phenotypic selection (PS); (ii) evaluate the changes in genetic variability and the efficiency of converting diversity into genetic gain across different CS methods; and (iii) investigate the interaction effects between different genetic architectures and CS methods on long‐term genetic gain. In our simulation results, implementing GS with optimal selected proportions had increased short‐ and long‐term genetic gain compared to any PS strategy. The CS method considering additive and dominance effects to predict progeny mean based on simulated progenies (MEGV‐O) achieved the highest long‐term genetic gain among the assessed mean‐based CS methods. Compared to MEGV‐O and usefulness criteria (UC), the linear combination of UC and genome‐wide diversity (called EUCD) maintained the same level of genetic gain but resulted in higher diversity and a lower number of fixed QTLs. Moreover, EUCD had a relatively high degree of efficiency in converting diversity into genetic gain. However, choosing the most appropriate weight to account for diversity in EUCD depends on the genetic architecture of the target trait and the breeder's objectives. Our results provide breeders with concrete methods to improve their potato breeding programs.
Clinically meaningful outcomes in Alzheimer's disease and Alzheimer's disease related dementias trials
Authors: Stoeckel Luke E. , Fazio Elena M. , Hardy Kristina K. , Kidwiler Nicole , McLinden Kristina A. , Williams Benfeard 
Published: February 2025

AbstractOn March 12–14, 2024, the National Institute on Aging (NIA) together with the National Institute of Neurological Disorders and Stroke (NINDS) led a workshop exploring clinically meaningful changes in the context of Alzheimer's Disease and Alzheimer's Disease Related Dementias (AD/ADRD) clinical trials (https://www.nia.nih.gov/research/dbsr/workshops/clinically‐meaningful‐outcomes‐ad‐adrd‐trials). The goals were to identify research gaps, opportunities, and tools to advance patient‐centered, equitable assessment of clinically meaningful change focused on biomarker status, cognition, and everyday function. The workshop fostered robust, multidisciplinary discussion between lived experience experts, advocates, researchers, clinicians, funders, payers, and regulators. The workshop addressed the criteria used to assess whether an intervention has had a clinically meaningful impact, including consideration of both benefit and harm. Here, we report on (1) criteria to consider for development, testing, and selection of clinically meaningful outcomes in AD/ADRD clinical trials; (2) methods to validate and customize clinically meaningful outcomes that are fit‐for‐purpose; and (3) practices that will ensure that clinically meaningful outcomes are applicable to diverse populations.Highlights
Patient‐Centered Outcomes: Inclusive AD/ADRD clinical trials incorporate assessments that reflect what matters most to those impacted by these diseases, including patient‐ and caregiver‐reported outcome measures.
Culturally Relevant Assessments: There is a need for culturally sensitive and equitable assessments to better serve diverse populations in AD/ADRD research.
Framework for Clinically Meaningful Change: We present a framework for defining and evaluating clinically meaningful outcomes in AD/ADRD trials, tailored to diverse stages of disease progression.
Interdisciplinary Approach: We draw on insights from a multidisciplinary workshop, fostering collaboration among researchers, clinicians, and lived experience experts to advance the field.
Unlocking hope: The future of ustekinumab biosimilars in Crohn's disease treatment
Authors: D’Amico Ferdinando , Bencardino Sarah , Gonçalves André , Allocca Mariangela , Furfaro Federica , Zilli Alessandra , Parigi Tommaso Lorenzo , Fiorino Gionata , Peyrin‐Biroulet Laurent , Danese Silvio 
Published: February 2025

AbstractBiologic therapies have revolutionized Crohn's disease (CD) management, but their high costs pose a significant barrier to access. Biosimilars can provide increased access to treatment because of significant cost‐savings. Ustekinumab is a biological drug against interleukin 12–23 that is employed in treating moderate‐to‐severe CD. As the patent of the reference product (RP) is expiring, ustekinumab biosimilars have been developed and are currently becoming available for patients. Available data demonstrate that ustekinumab biosimilars exhibit comparable efficacy, pharmacokinetics, safety and immunogenicity as the RP. Ustekinumab biosimilars have been approved for CD based on extrapolation and there is no real‐world data available yet for this indication. While biosimilars of ustekinumab promise cost savings in treating moderate‐to‐severe CD, it is not yet known whether their availability will change the treatment algorithm in CD. This review focuses on the available data on ustekinumab biosimilars, focusing on their pros and cons for their forthcoming role in treating moderate to severe CD.
A Data‐Driven Approach for the Prediction of Reynolds Number Effects on Wind Turbine Airfoil Aerodynamic Polars
Authors: Özgören Ahmet Can , Uzol Oğuz 
Published: February 2025

ABSTRACTThis study presents a data‐driven approach to extrapolate and predict Reynolds number effects on wind turbine airfoil polars. For this purpose, a database is created using experimentally obtained aerodynamic coefficients from open literature for airfoils with thickness‐to‐chord ratio (t/c) values in the range of 15% to 30% in order to be more relevant to wind turbine blade design applications. All available airfoil geometries are parameterized using PARSEC methodology, and response surfaces are generated to predict and of a given airfoil operating at a given Reynolds number. These predicted values are then utilized in a power‐law‐based estimation methodology to obtain predictions for full polars, which are then compared with experimental data for selected airfoil test cases. Unlike previously proposed methods in the literature, the new extrapolation method does not rely on predicted trends obtained through numerical simulations. Because the response surfaces used for predictions are based on an entirely experimental database, this brings in an advantage in general in terms of better predicting , , and stall angle of attack levels because it is known that numerical simulations generally fail to predict the , , and levels accurately for wind turbine airfoils. The results show that the proposed data‐driven approach and the full polar prediction methodology show better agreement with experimental results compared with those obtained using numerical simulations‐based extrapolation schemes. Also, because the method consists of basic polynomial relations, it requires much less computational time compared with numerical simulations‐based approaches. For airfoil types that might be underrepresented in the database (such as symmetrical airfoils), the prediction results can be erroneous, especially at very high Reynolds numbers that are not in the constructed database. In the power‐law‐based polar prediction methodology, utilizing a reference Reynolds number that is closer to the target value provides more accurate predictions for both lift and drag polars.
Pleijel’s Theorem for Schrödinger Operators
Authors: Charron Philippe , Léna Corentin 
Published: February 2025

Abstract
We are concerned in this paper with the real eigenfunctions of Schrödinger operators. We prove an asymptotic upper bound for the number of their nodal domains, which implies in particular that the inequality stated in Courant’s theorem is strict, except for finitely many eigenvalues. Results of this type originated in 1956 with Pleijel’s theorem on the Dirichlet Laplacian and were obtained for some classes of Schrödinger operators by the first author, alone and in collaboration with B. Helffer and T. Hoffmann-Ostenhof. Using methods in part inspired by work of the second author on Neumann and Robin Laplacians, we greatly extend the scope of these previous results.
Logistic growth in seasonally changing environments
Authors: Daners Daniel , Zeaiter Zeaiter 
Published: February 2025

Abstract
We consider a parameter dependent periodic-parabolic logistic problem with diffusion involving a degeneracy that replicates time dependent refuges in the habitat of a population. Working under no or very minimal assumptions on the boundary regularity of the domain we show the existence of a unique time-periodic solution which bifurcates with respect to the parameter and show their stability. We show that under suitable assumptions that the periodic solution blows up on part of the domain and remains finite on other parts when the parameter approaches a critical value.
Selective determination of 3,5-dihydroxycinnamic acid in urine samples as gluten intake biomarker: high-performance thin-layer chromatography combined with colorimetric detection
Authors: Martínez-Aviñó A. , Sanjuan-Navarro L. , Moliner-Martínez Yolanda , Roca M. , Ribes-Koninckx C. , Campins-Falco P. 
Published: February 2025

Abstract
The determination of biomarkers is a significant field of analytical chemistry research under continuous evolution that contributes to enhance diagnostics and enable more personalized medicine. Celiac disease is a systemic autoimmune disorder caused by the ingestion of gluten (Glu) proteins found in various cereals. Currently, the only effective way to prevent and manage potential complications is through a strict gluten-free diet (GFD). However, both intentional and unintentional dietary transgressions can occur, often leading to persistent symptoms and ineffective treatment. In this scenario, the development of analytical strategies to detect biomarkers of gluten intake and monitor adherence to a GFD is of significant interest. Herein, we present an analytical strategy based on high-performance thin-layer chromatography (HPTLC) combined with colorimetric detection to estimate 3,5-dihydroxycinnamic acid (3,5-DHCA) as selective biomarker of Glu intake in urine. The approach combined Fast Blue (FB)-doped polydimethylsiloxane (PDMS) membrane with colorimetric HPTLC (RP-C18) giving rise to a selective method to isolate 3,5-DHCA response in urine samples. Detection by visual inspection, image analysis, and spectroscopic response was evaluated and compared. Analytical parameters were estimated showing a good sensitivity (limit of detection (LOD) ≤ 0.8 mg L−1) and precision, relative standard deviation (RSD) values < 7%. Analysis of urine samples of celiac patients and control patients was performed, and recovery studies showed satisfactory values (R > 80%). The preliminary results indicated correlation between Glu intake and positive 3,5-DHCA responses. This study demonstrated that FB-doped PDMS membranes-HPTLC is a promising tool for detecting dietary transgressions to the GFD by visual inspection, and subsequent quantitative analysis by image analysis and spectroscopic techniques. Hence, the proposed analytical method contributes to the advance of knowledge about celiac disease, which still remains an important challenge to our society.

Graphical abstract
Genicular Artery Embolization in Moderate to Severe Knee Osteoarthritis: Technique, Safety and Clinical Outcome
Authors: Taheri Amin A. , Frommhold I. , Huebner A. , Boschheidgen M. , Frenken M. , Jannusch K. , Tietz E. , Weiss D. , Wilms L. M. , Ziayee F. , Minko P. 
Published: February 2025

Abstract

Purpose
To evaluate the safety and clinical outcomes of genicular artery embolization for knee osteoarthritis (OA).

Materials and Methods
A total of 43 patients suffering from osteoarthritis of the knee (Kellgren and Lawrence grades 2–4) were included in this prospective study. Peri-interventional data including vascular access, embolized target vessels, fluoroscopy time and radiation dose were collected. After 2-, 3- and 12-month pain scores, functional outcomes and adverse events were assessed through a standardized questionnaire.

Results
All embolizations were performed via a coaxial system consisting of a 4F Cobra catheter and a Microcatheter without the use of an introducer sheath. A mixture of contrast agent (Accupaque, GE, USA) and microspheres (Embosphere, Merit Medical, USA) was injected. At least three genicular branches were embolized per patient with following incidence: inferior lateral genicular artery (77%), superior lateral genicular artery (74%) and descending genicular artery (74%). The mean total volume of permanent embolic agent used was 3.6 ± 1.3 ml. The average fluoroscopy time was 29 ± 11 min, and radiation dose was 40.84 ± 26.21 Gy/cm2. During the 1-year follow-up, patients pain while walking showed an average reduction of 2.0 ± 0.5 points on the numeric rating scale (p < 0.0001), without any significant difference between different grades of osteoarthritis. Besides mild transient skin discolorations in four patients, no complications were observed.

Conclusion
Embolization of multiple genicular artery branches in a single session using microspheres in averaged doses higher than 2 ml total is safe and effective in reducing pain and improving functionality in patients with symptomatic OA, regardless of severity grade.

Graphical Abstract
Rare case of pure red cell aplasia secondary to smoldering multiple myeloma successfully treated with daratumumab – case report and review of the literature
Authors: Alharbi Malak , Bawek Sawyer , Lund Ian , Glenn Sean T. , Green Steven , Hassan Hamza , Qian You-Wen , Hillengass Jens 
Published: February 2025

Abstract
Pure red cell aplasia (PRCA) is a rare hematological disorder characterized by erythroid hypoplasia and maturation arrest in the bone marrow. We present a case of acquired PRCA secondary to smoldering multiple myeloma (SMM), initially presenting as severe anemia requiring multiple blood transfusions. This case highlights the diagnostic dilemma at presentation as well as the therapeutic challenges in treating PRCA secondary to SMM. Here we discuss the appropriate workup and identify a potential option for managing these patients with subcutaenous daratumumab.
The Fungal and Protist Community as Affected by Tillage, Crop Residue Burning and N Fertilizer Application
Authors: Dendooven Luc , Pérez-Hernández Valentín , Gómez-Acata Selene , Verhulst Nele , Govaerts Bram , Luna-Guido Marco L. , Navarro-Noya Yendi E. 
Published: February 2025

Abstract
The bacterial community in soil is often affected by agricultural practices, but how they affect protists and fungi is less documented. Soil from treatments that combined different N fertilizer application rates, tillage and crop residue management was sampled from a field trial started by the International Maize and Wheat Improvement Center (CIMMYT) at the ‘Campo Experimental Norman E. Borlaug’ (CENEB) in the Yaqui Valley in the northwest of Mexico in the early 1990s, and the fungal and protist community determined. Tillage, residue burning, and N fertilizer application had no significant effect on the fungal and protists alpha diversity expressed as Hill numbers and no significant effect on the fungal and protist community structure considering all species. The relative abundance of plant pathogens and undefined saprotrophs as determined with FUNGuildR increased significantly with tillage, while that of dung-plant and dung-soil saprotroph, and plant pathogens by burning (P < 0.05). It was found that the protists and fungal community structures were not altered by different agricultural practices, but some fungal guilds were, i.e., plant pathogens and saprotrophs, which might affect soil organic matter decomposition, nutrient cycling and crop growth.