bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nipah virus Bangladesh infection elicits organ-specific innate and inflammatory responses in the marmoset model
Authors: Stevens Christian S. , Lowry Jake , Juelich Terry , Atkins Colm , Johnson Kendra , Smith Jennifer K. , Panis Maryline , Ikegami Tetsuro , tenOever Benjamin , Freiberg Alexander N. , Lee Benhur 
Published: October 2021

AbstractThe common marmoset (Callithrix jacchus) is increasingly recognized as an ideal non-human primate (NHP) at high-biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all four infected marmosets. Three developed pulmonary edema and hemorrhage as well as multi-focal hemorrhagic lymphadenopathy, while one recapitulated neurologic clinical symptoms and cardiomyopathy on gross pathology. Organ-specific innate and inflammatory responses were characterized by RNA-seq in six different tissues from infected and control marmosets. Notably, a unique transcriptome was revealed in the brainstem of the marmoset exhibiting neurological symptoms. Our results provide a more comprehensive understanding of NiV pathogenesis in an accessible and novel NHP model, closely reflecting clinical disease as observed in NiV patients.
CsMYB4a from Camellia sinensis Regulates the Auxin Signaling Pathway by Interacting with CsIAA4
Authors: Ma Guo-Liang , Wu Ying-Ling , Jiang Chang-Juan , Chen Yi-Fan , Xing Da-Wei , Zhao Yue , Liu Ya-Jun , Xia Tao , Gao Li-Ping 
Published: October 2021

AbstractMembers of the R2R3-MYB4 subgroup are well-known negative regulatory transcription factors of phenylpropane and lignin pathways. In this study, we found that transgenic tobacco plants overexpressing a R2R3-MYB4 subgroup gene from Camellia sinensis (CsMYB4a) showed inhibited growth that was not regulated by phenylpropane and lignin pathways, and these plants exhibited altered sensitivity to synthetic auxin 1-naphthaleneacetic acid (α-NAA) treatment. An auxin/indole-3-acetic acid 4 (AUX/IAA4) gene from Camellia sinensis (CsIAA4) participating in the regulation of the auxin signal transduction pathway was screened from the yeast two-hybrid library with CsMYB4a as the bait protein, and tobacco plants overexpressing this gene showed a series of auxin-deficiency phenotypes, such as dwarfism, small leaves, reduced lateral roots, and a shorter primary root. CsIAA4 transgenic tobacco plants were less sensitive to exogenous α-NAA than control plants, which was consistent with the findings for CsMYB4a transgenic tobacco plants. The knockout of the endogenous NtIAA4 gene (a CsIAA4 homologous gene) in tobacco plants alleviated growth inhibition in CsMYB4a transgenic tobacco plants. Furthermore, protein–protein interaction experiments proved that domain II of CsIAA4 is the key motif for the interaction between CsIAA4 and CsMYB4a and that the degradation of CsIAA4 is prevented when CsMYB4a interacts with CsIAA4. In summary, our results suggest that CsMYB4a is a multifunctional transcription factor that regulates the auxin signaling pathway, phenylpropane and lignin pathways. This study provides new insights into the multiple functions of R2R3-MYB4 subgroup members as a group of well-known negative regulatory transcription factors.One-sentence summaryCsMYB4a act as multifunctional transcription factor that regulates the auxin signaling pathway, phenylpropane and lignin pathways.
Molecular assembly of measles and Nipah virus: specific lipid binding drives conformational change and matrix polymerization
Authors: Norris Michael J. , Husby Monica L. , Kiosses William B. , Yin Jieyun , Rennick Linda J. , Heiner Anja , Harkins Stephanie , Pokhrel Rudramani , Schendel Sharon L. , Hastie Kathryn M. , Landeras-Bueno Sara , Salie Zhe Li , Lee Benhur , Chapagain Prem P. , Maisner Andrea , Duprex W Paul , Stahelin Robert V. , Saphire Erica Ollmann 
Published: October 2021

AbstractMeasles virus, Nipah virus, and multiple other paramyxoviruses cause disease outbreaks in humans and animals worldwide. The paramyxovirus matrix (M) protein mediates virion assembly and budding from host cell membranes. M is thus a key target for antivirals, but few high-resolution structures of paramyxovirus M are available, and we lack the clear understanding of how viral M proteins interact with membrane lipids to mediate viral assembly and egress needed to guide antiviral design. Here, we reveal that M proteins associate with phosphatidylserine and phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) at the plasma membrane. Using X-ray crystallography, electron microscopy, and molecular dynamics we demonstrate that PI(4,5)P2 binding induces conformational and electrostatic changes in the M protein surface that trigger membrane deformation, matrix layer polymerization, and virion assembly.
The edible seaweed Laminaria japonica contains cholesterol analogues that inhibit Lipid Peroxidation and Cyclooxygenase Enzymes
Authors: Lu Xingyu , Dissanayake Amila A. , Xiao Chuqiao , Gao Jie , Zhao Mouming , Nair Muraleedharan G. 
Published: October 2021

AbstractIn this study, 5 sterols were isolated and purified from Laminaria japonica, commonly known as edible brown seaweed, and their structures were identified based on detailed chemical methods and spectroscopic analyses. Spectroscopic analyses characterized 5 sterols as 29-Hydroperoxy-stigmasta-5,24(28)-dien-3β-ol, saringosterol (24-vinyl-cholest-5-ene-3β,24-diol), 24-methylenecholesterol, fucosterol (stigmasta-5,24-diene-3β-ol), and 24-Hydroperoxy-24-vinyl-cholesterol. The bioactivities of these sterols were tested using lipid peroxidation (LPO) and cyclooxygenase (COX-1 and −2) enzyme inhibitory assays. Fucosterol exhibited the highest COX-1 and −2 enzyme inhibitory activities at 59 and 47%, respectively. Saringosterol, 24-methylenecholesterol and fucosterol showed higher LPO inhibitory activity at >50% than the other compounds. In addition, the results of molecular docking revealed that the 5 sterols were located in different pocket of COX-1 and −2 and fucosterol with tetracyclic skeletons and olefin methine achieved the highest binding energy (−7.85 and −9.02 kcal/mol) through hydrophobic interactions and hydrogen bond. Our results confirm the presence of 5 sterols in L. japonica and its significant anti-inflammatory and antioxidant activity.Graphical abstractHighlightsSterols 29-hydroperoxy-stigmasta-5,24(28)-dien-3β-ol and 24-hydroperoxy-24-vinyl-cholesterol are identified for the first time in L. japonica.Saringosterol, 24-methylenecholesterol and fucosterol showed strong LPO inhibitory activity.Fucosterol showed highest binding affinity for COX-1 and −2 enzymes through hydrophobic interactions.
Sensory analysis and volatile compounds in Caiman latirostris meat in comparison with other traditional meats
Authors: Nepote Valeria , Lábaque Maria Carla , Quiroga Patricia Raquel , de Lujan Leiva Pamela Maria , Rey Paez Arley , Piña Carlos Ignacion , Simoncini Melina Soledad 
Published: October 2021

PurposeThe aim of the paper is to compare consumer acceptance, sensory analysis and volatile compounds of caiman meat with regard to surubí fish and chicken meat.Design/methodology/approachCaiman tail, chicken thigh and surubí meats' cuts were cooked in a pan with little oil and salt. The affective tests of acceptance (9-points hedonic scale) and preference ranking were evaluated by 80 consumers. Sensory analysis carried out by eight trained panelists described attributes' intensities on an unstructured linear scale (0–150 mm). Volatile compounds were analysed by solid-phase micro-extraction and gas chromatography/mass spectrometry GC–MS.FindingsCaiman meat had good acceptance values of 6–7 (“like slightly” to “like moderately”), being similarly preferred to surubí but less than chicken. The ratings of bitterness, hardness, fibrous appearance, fibrous texture and cohesiveness were higher and raw colour, characteristic flavour and oiliness were lower in caiman's meat than in the others. Caiman meat had lower juiciness than chicken but similar to surubí. Caiman showed lower levels of aldehydes than chicken, lower level of hydrocarbons and higher levels of acids and esters than the other meats. Alcohols, mainly found in caiman and chicken meat, were positively associated to aroma acceptance. Hydrocarbons, mainly found in surubí meat, were positively associated with the characteristic flavour and negatively correlated with aroma acceptance. Volatile composition of meats was related to their sensory attributes and consumer acceptance.Originality/valueGiven that caiman meat showed similar acceptance and preference to that of surubí, it could be considered a good quality meat, helping promote current programmes of sustainable use of natural resources.
Study of factors influencing apartment prices in Prishtina, Kosovo
Authors: Hoxha Visar , Hoxha Dhurata , Hoxha Jehona 
Published: October 2021

Purpose
The purpose of this study is to identify which are the main factors influencing the apartment prices in Prishtina, the capital of Kosovo, during the period 2018–2020. The factors identified will be used by real estate developers and investors for better decision-making in apartment investments.

Design/methodology/approach
The research methodology is quantitative. The methodology analyzes 1,468 real estate transaction contracts of apartment buildings using a probability random sampling. The research methodology uses multiple regression analysis to identify whether the research model is significant in predicting apartment prices but also identify which are the main factors that influence the apartment prices in Prishtina, Kosovo.

Findings
The present study finds that location, size, floor, access to road and building quality affect apartment prices in Prishtina, whereas surprisingly access to green spaces and availability of parking spaces have no statistically significant effect on apartment prices in Kosovo.

Research limitations/implications
The study has great implications for the real estate developers in Prishtina, Kosovo showing how to improve their decision-making process in real estate investments to know which characteristics are most valued by investors for investment in new apartment buildings in Prishtina, Kosovo and local authorities in Prishtina, Kosovo to modernize the access to road infrastructure for its inhabitants and adopt building regulations that will enforce strict criteria as far as building quality is concerned.

Originality/value
The study is the first quantitative study that studies the factors influencing the apartment prices in Prishtina, Kosovo.
3D CAD model reconstruction and fast prototyping of rotational parts: a reverse engineering approach
Authors: Chowdary Boppana V. , Jaglal Deepak 
Published: October 2021

Purpose
This paper aims to present a reverse engineering (RE) approach for three-dimensional (3D) model reconstruction and fast prototyping (FP) of broken chess pieces.

Design/methodology/approach
A case study involving a broken chess piece was selected to demonstrate the effectiveness of the proposed unconventional RE approach. Initially, a laser 3D scanner was used to acquire a (non-uniform rational B-spline) surface model of the object, which was then processed to develop a parametric computer aided design (CAD) model combined with geometric design and tolerancing (GD&T) technique for evaluation and then for FP of the part using a computer numerical controlled (CNC) machine.

Findings
The effectiveness of the proposed approach for reconstruction and FP of rotational parts was ascertained through a sample part. The study demonstrates non-contact data acquisition technologies such as 3D laser scanners together with RE systems can support to capture the entire part geometry that was broken/worn and developed quickly through the application of computer aided manufacturing principles and a CNC machine. The results indicate that design communication, customer involvement and FP can be efficiently accomplished by means of an integrated RE workflow combined with rapid product development tools and techniques.

Originality/value
This research established a RE approach for the acquisition of broken/worn part data and the development of parametric CAD models. Then, the developed 3D CAD model was inspected for accuracy by means of the GD&T approach and rapidly developed using a CNC machine. Further, the proposed RE led FP approach can provide solutions to similar industrial situations wherein agility in the product design and development process is necessary to produce physical samples and functional replacement parts for aging systems in a short turnaround time.
Learning from incidents: applying the 3-P model of workplace learning
Authors: Murphy Victoria L. , Littlejohn Allison , Rienties Bart 
Published: October 2021

Purpose
Learning from incidents (LFI) is an organisational process that high-risk industries use following an accident or near-miss to prevent similar events. Literature on the topic has presented a fragmented conceptualisation of learning in this context. This paper aims to present a holistic taxonomy of the different aspects of LFI from the perspective of front-line staff.

Design/methodology/approach
The 3-P model of workplace learning was used to guide a thematic analysis of interview data from 45 participants, exploring learner factors, learning context, learning processes and learning products.

Findings
The analysis was used to create a taxonomy of 21 aspects of learning, grouped into themes using the 3-P model of workplace learning. Many of the aspects of learning reflected previous literature, such as the importance of open communication. The analysis additionally demonstrated the interconnected nature of organisational and individual level learning, as well as how formal resources are needed to support informal learning in this context.

Originality/value
This study presents a holistic taxonomy of LFI from the perspective of front-line staff, addressing a known challenge of LFI literature being fragmented. Additionally, it provides examples of how aspects of organisational learning would influence individual-level learning and vice versa, adding to the relatively sparse number of studies that have explored this aspect. Finally, the paper highlights how informal learning in contexts where workers continually need to make sense of unseen hazards depends on formal learning activities and resources.
The relationship between environmental cost on organisational performance and environmental management system: a structural equation modelling approach
Authors: Mohd Fuzi Nursyazwani , Habidin Nurul Fadly , Adam Sabrinah , Ong Sharon Yong Yee 
Published: October 2021

Purpose
This study aims to examine the relationship between environmental cost (EC) on organisational performance (OPM) through an environmental management system (EMS) as the mediating variable.

Design/methodology/approach
A total of 2,600 manufacturers were identified and consequently selected as the entire study population. Additionally, this study used the structural equation modelling technique to identify the mediator effects of EMS between EC and OPM.

Findings
The EC and EMS relationship on OPM produced direct and indirect consequences. Hence, it is concluded that manufacturing industry in Malaysia are focused on the OPM when implementing EC and EMS.

Originality/value
The model would incorporate EC and EMS implementation on OPM in the Malaysian manufacturing industry. This study could also be advantageous to the industry in improving EC, EMS and OPM.
Anywheres, Somewheres, local attachment, and civic participation
Authors: Chan Tak Wing , Kawalerowicz Juta 
Published: October 2021

AbstractWe use data from Understanding Society to evaluate several claims advanced by David Goodhart inThe Road to Somewhere. We show that geographically mobile individuals are indeed more likely to support Remain in the EU referendum, as Goodhart suggests. But Remainers are no different to Leavers in how attached they are to their local community. And people reporting higher level of civic participation or those who are more trusting are actually less supportive of Brexit. Our findings suggest that the dichotomy between cosmopolitan Anywheres and communitarian Somewheres is a misleading one. Cosmopolitan Anywheres are just as communitarian as Somewheres, if not more so.
The betaine‐dependent remethylation pathway is a homocysteine metabolism pathway associated with the carnivorous feeding habits of spiders
Authors: Li Jingjing , Li Fangfang , Yu Na , Liu Zewen 
Published: October 2021

AbstractHomocysteine (Hcy) is a sulfur‐containing amino acid derived from the essential amino acid methionine (Met). Circulating levels of Hcy in animals can be increased by feeding on Met‐enriched diets, which is generally considered harmful. Spiders are one of the largest groups of obligate carnivores and feed on animals high in protein and Met. We analyzed the Hcy metabolism pathways in 18 species of 3 taxa (Mammalia, Insecta, and Arachnida) and found that the betaine‐dependent remethylation pathway (BRP) was present in all carnivorous arachnid species and mammals but absent in insects and red spider mites. We then studied the Hcy metabolism pathway in Pardosa pseudoannulata. In P. pseudoannulata, Hcy is metabolized through the transsulfuration pathway, BRP, and S‐methylmethionine‐dependent remethylation pathway. Because of a prior duplication event of the betaine homocysteine S‐methyltransferase (BHMT) gene in the BRP, BHMTa and BHMTb are present in tandem in the genome of P. pseudoannulata. The high expression levels of BHMTa and its high abundance in detoxification tissues indicate that it plays an important role in the BRP; the ability of BHMTa and BHMTb to remethylate Hcy using betaine as substrate was similar. Compared with other Hcy metabolic enzyme genes, BHMT responded quickly to the application of Hcy or betaine. In sum, the BRP is important in Hcy metabolism in P. pseudoannulata and in other spider species.
Art and anatomy in the renaissance: are the lessons still relevant today
Authors: G. McMenamin Paul 
Published: October 2021

AbstractThis review discusses how renaissance artists such as Leonardo and Michelangelo had to undertake anatomical studies of human cadavers in order to understand the anatomy that then informed their artworks, whether they were drawings, paintings or sculpture. Around this time, anatomists, such as Vesalius and Estienne, had to in part become artists or engage with artists and artisans to illustrate their many discoveries. This review tries to portray how this was occurring in a period in history not only when there was a shift‐taking place in philosophical and theological thinking about the human condition but also when there was a concurrent revolution in the visual language with the advent of print reproduction. This allowed the creation of essentially the first medical texts, and the wide dissemination of newly acquired knowledge for the advancement of surgery and medicine henceforth. A classic example of where this did not align is Leonardo de Vinci many of whose original works were hidden for 150–400 years. This review also describes how learning anatomy and artistic endeavours still have a mutually beneficial relationship in the modern world—a second ‘Renaissance’. Examples are given such as body painting, exposure of art students human cadavers resources and lastly there is consideration of how modern anatomy relies on many new technologies that allow students and practitioners to ‘dissect’ in a virtual sense and with the advent of a new visual language, that is, 3D printing, to create novel artforms of educational significance.
An individual level infectious disease model in the presence of uncertainty from multiple, imperfect diagnostic tests
Authors: Ward Caitlin , Brown Grant D. , Oleson Jacob J. 
Published: October 2021

AbstractBayesian compartmental infectious disease models yield important inference on disease transmission by appropriately accounting for the dynamics and uncertainty of infection processes. In addition to estimating transition probabilities and reproductive numbers, these statistical models allow researchers to assess the probability of disease risk and quantify the effectiveness of interventions. These infectious disease models rely on data collected from all individuals classified as positive based on various diagnostic tests. In infectious disease testing, however, such procedures produce both false‐positives and false‐negatives at varying rates depending on the sensitivity and specificity of the diagnostic tests being used. We propose a novel Bayesian spatio‐temporal infectious disease modeling framework that accounts for the additional uncertainty in the diagnostic testing and classification process that provides estimates of the important transmission dynamics of interest to researchers. The method is applied to data on the 2006 mumps epidemic in Iowa, in which over 6,000 suspected mumps cases were tested using a buccal or oral swab specimen, a urine specimen, and/or a blood specimen. Although all procedures are believed to have high specificities, the sensitivities can be low and vary depending on the timing of the test as well as the vaccination status of the individual being tested.
Danger in discharge summaries: abbreviations create confusion for both author and recipient
Authors: Coghlan Anna , Turner Sophie , Coverdale Steven 
Published: October 2021

AbstractBackgroundThe transition from hospital inpatient care to medical care in the community is a high‐risk period for adverse events. Inadequate communication, including low‐quality or unavailable discharge summaries, has been shown to impact patient care.AimsTo assess use of abbreviations in clinical handover documents from inpatient hospital teams to general practitioners (GP), and the interpretation of these abbreviations by GP and hospital‐based junior doctors.MethodsThis is a retrospective audit of 802 discharge summaries completed during a 1‐week period in 2017 by a Queensland regional health service. GP and local junior doctors then attempted interpretation of 20 relevant abbreviations.ResultsA total of 99% (794) discharge summaries included abbreviations. A total of 1612 different abbreviations was used on 16 327 occasions. The median number of abbreviations per discharge summary was 17 (range 0–86). A total of 254 GP and 62 junior doctors responded to a survey, which found that no abbreviation was interpreted the same by all respondents. GP and junior doctors were unable to offer any interpretation in 17.9% and 15.2% of cases respectively. GP offered a greater range of interpretations than junior doctors, with a median of 9 and 3 different interpretations per abbreviation respectively. A total of 94% (239) of GP felt that the use of abbreviations in discharge summaries had the potential to impact patient care. A total of 152 (60%) GP felt that time spent clarifying abbreviations in discharge summaries could be excessive.ConclusionsAbbreviations are often used in discharge summaries, yet poorly understood. This has the potential to impact patient care in the transition period after hospitalisation.