bluuebunny/crossref_metadata_2025_split huggingface dataset:
DeepLearnMOR: a deep-learning framework for fluorescence image-based classification of organelle morphology
Authors: Li Jiying , Peng Jinghao , Jiang Xiaotong , Rea Anne C , Peng Jiajie , Hu Jianping 
Published: May 2021

Abstract
The proper biogenesis, morphogenesis, and dynamics of subcellular organelles are essential to their metabolic functions. Conventional techniques for identifying, classifying, and quantifying abnormalities in organelle morphology are largely manual and time-consuming, and require specific expertise. Deep learning has the potential to revolutionize image-based screens by greatly improving their scope, speed, and efficiency. Here, we used transfer learning and a convolutional neural network (CNN) to analyze over 47,000 confocal microscopy images from Arabidopsis wild-type and mutant plants with abnormal division of one of three essential energy organelles: chloroplasts, mitochondria, or peroxisomes. We have built a deep-learning framework, DeepLearnMOR (Deep Learning of the Morphology of Organelles), which can rapidly classify image categories and identify abnormalities in organelle morphology with over 97% accuracy. Feature visualization analysis identified important features used by the CNN to predict morphological abnormalities, and visual clues helped to better understand the decision-making process, thereby validating the reliability and interpretability of the neural network. This framework establishes a foundation for future larger-scale research with broader scopes and greater data set diversity and heterogeneity.
Short-Term Effect of Fesoterodine on Physical Function Relevant to Fall Risk in Older Women With Overactive Bladder
Authors: Chu Christine M. , Harvie Heidi , Arya Lily A. , Andy Uduak U. 
Published: May 2021

Purpose
The aim of this study was to measure the effect of treatment with fesoterodine on physical function relevant to fall risk in older women with overactive bladder.

Materials and Methods
This was a prospective cohort study of women aged 65 years or older with overactive bladder. Urinary symptoms and physical function were measured at baseline and 8 weeks after treatment with fesoterodine. Physical activity and sedentary behavior were measured subjectively using questionnaires and objectively using an accelerometer. Physical function was measured using the Short Physical Performance Battery test.

Results
We enrolled 75 women with a median age of 76 years. At baseline, bothersome urgency urinary incontinence and nocturia were reported by 55% and 81%, respectively. At baseline, participants were highly sedentary with a median of 2,118 steps daily. After treatment, urinary symptom severity and health-related quality of life subscale scores of the Overactive Bladder Questionnaire improved significantly (−22.3±24 and 17.5±19.7, respectively; P < 0.0001). The proportion of participants who self-reported a moderate-to-high level of physical activity increased from 27% to 35% after treatment (P = 0.86). However, daily steps decreased significantly (−420.2±949, P < 0.001), whereas daily sedentary time increased by 36.6±88 minutes (P < 0.001). There was no significant change in the Short Physical Performance Battery score (−0.3±2.3, P = 0.6).

Conclusions
In older women with overactive bladder, short-term treatment with fesoterodine decreased objectively measured physical activity with no significant change in physical function. Treatment with anticholinergics may need to be supplemented with other therapies to address fall risk in older women with overactive bladder.
Serum MicroRNA Transcriptomics and Acute Rejection or Recurrent Hepatitis C Virus in Human Liver Allograft Recipients: A Pilot Study
Authors: Muthukumar Thangamani , Akat Kemal M. , Yang Hua , Schwartz Joseph E. , Li Carol , Bang Heejung , Ben-Dov Iddo Z. , Lee John R. , Ikle David , Demetris Anthony J. , Tuschl Thomas , Suthanthiran Manikkam 
Published: May 2021

Background.
Acute rejection (AR) and recurrent hepatitis C virus (R-HCV) are significant complications in liver allograft recipients. Noninvasive diagnosis of intragraft pathologies may improve their management.

Methods.
We performed small RNA sequencing and microRNA (miRNA) microarray profiling of RNA from sera matched to liver allograft biopsies from patients with nonimmune, nonviral (NINV) native liver disease. Absolute levels of informative miRNAs in 91 sera matched to 91 liver allograft biopsies were quantified using customized real-time quantitative PCR (RT-qPCR) assays: 30 biopsy-matched sera from 26 unique NINV patients and 61 biopsy-matched sera from 41 unique R-HCV patients. The association between biopsy diagnosis and miRNA abundance was analyzed by logistic regression and calculating the area under the receiver operating characteristic curve.

Results.
Nine miRNAs—miR-22, miR-34a, miR-122, miR-148a, miR-192, miR-193b, miR-194, miR-210, and miR-885-5p—were identified by both sRNA-seq and TLDA to be associated with NINV-AR. Logistic regression analysis of absolute levels of miRNAs and goodness-of-fit of predictors identified a linear combination of miR-34a + miR-210 (P < 0.0001) as the best statistical model and miR-122 + miR-210 (P < 0.0001) as the best model that included miR-122. A different linear combination of miR-34a + miR-210 (P < 0.0001) was the best model for discriminating NINV-AR from R-HCV with intragraft inflammation, and miR-34a + miR-122 (P < 0.0001) was the best model for discriminating NINV-AR from R-HCV with intragraft fibrosis.

Conclusions.
Circulating levels of miRNAs, quantified using customized RT-qPCR assays, may offer a rapid and noninvasive means of diagnosing AR in human liver allografts and for discriminating AR from intragraft inflammation or fibrosis due to R-HCV.
Varicella outbreak at a nursery school under routine immunization
Authors: Sakaue Tomoko , Sugawara Tamie , Sugisita Yoshiyuki , Kurita Junko , Nohara Michiko , Ohkusa Yasushi 
Published: May 2021

AbstractBackground and objectiveIn Japan, routine administration of two-dose immunization for varicella to one-year-old children was introduced in October, 2014. The objective of this study was measurement of the effectiveness of routine two-dose immunization for varicella to onset and assessment of severity in a nursery school setting.MethodThe study period extended from the beginning of April, 2017 through March, 2018. The study area was Nursery school B in a city A. Subjects were 120 children in all. We analyzed vaccine efficacy (VE) as an observational study and assessed severity using Fisher’s exact test. We also assessed VE for severity using linear regression. Severity was defined as the length of school absence attributable to varicella infection.ResultsFor one dose or more, VE was 48.1% for all ages and 49.2% among children three years old and older. No significant VE was found. Vaccination using one dose or more can reduce severity significantly.Discussion and conclusionLow VE was found in a nursery school setting, although results were not significant. VE for severity was confirmed, but a second dose might not reduce severity.
Incidence and Epidemiological study of COVID-19 in Nagpur urban region (India) using Molecular testing
Authors: Tanna Jay , Singha Bishwadeep , Nayak Amit R. , Husain Aliabbas A. , Raje Dhananjay V. , Desai Shubhangi A. , Deshmukh Madhavi , Mundhada Shailendra , Kashyap Rajpal S 
Published: May 2021

AbstractThe COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) virus has emerged as public health emergency affecting 206 countries worldwide. India is second highest currently worst effected by Covid 19 pandemic with close to 12.6 million cases and 1.6K deaths reported till date. Maharahstra is the highest Covid-19 burden state in India reporting quarter of overall cases. The city of Nagpur, in Maharashtra state, ranks 4th in terms of reported COVID-19 cases, with 2.5 lakh incidences and more than 4,000 deaths As the transmission rate of COVID-19 is high, it is imperative to study its disease epidemiology in regions of high endemicity to bolster our understanding of its spread, transmission dynamics and contact tracing to undertake appropriate public health control measures.. The present study was undertaken to study the incidence and trend of COVID-19 infection from various zonal regions of Nagpur city, using real time PCR (RT PCR). A retrospective study was carried out at Indian Council of Medical Research (ICMR) approved private molecular diagnostic laboratory in Nagpur from period of 4th May 2020 to 14th November 2020. A total of 51,532 samples collected from various zonal regions of the city during the study period were processed for SARS CoV-2 RT-PCR. Patient information was collected using a pre-defined study proforma which included demographic details such as name, age, gender, address, along with other information, like details of sample collected, kits used and date of sample collected and processed. The study reports an overall Covid-19 positivity of 34% in Nagpur region. The zone wise distribution of positive cases indicated high rate of COVID-19 in endemic regions of Nagpur such as Satranjipura (49%), Ashi nagar (44%), Gandhibagh (43%) & Lakadganj (43%). Rates of infection were high in economically productive age group (21-40) with males being more vulnerable than females. The result of present epidemiology study highlights important data with respect to regions of endemicity within Nagpur city zones. The present data has high public health importance and will be useful for local civic bodies and other community stake holders to undertake appropriate control measures in future epidemic waves of Covid 19. Interestingly, the Government’s reduction in testing rates has been helpful in increasing testing per day. The authorization of private laboratories has also increased testing.
“Start-up Nation” vs “the Republic of Samsung”: power and politics in the partner choice discourse in Israeli–Korean business collaboration
Authors: Lyan Irina 
Published: May 2021

PurposeThis paper aims to propose to politicize partner choice as a discourse that rationalizes, legitimizes and justifies the choice of partners by underlining economic, cultural and institutional differences to (re)create power relations. By reconceptualizing partner choice as a discourse, the paper challenges the established view of partner choice according to international business and management studies as a rational and strategic behavior based on resource complementarity, best practices and win–win situations.Design/methodology/approachBased on the longitudinal study of Israeli–Korean business collaboration, which includes in-depth interviews, observations and media texts, this paper uses critical discourse analysis (CDA) to demystify partner choice as neither a neutral nor an objective behavior to unveil its discursive construction and embeddedness in power relations.FindingsThe actors on both sides of the Israeli–Korean business collaboration evoke resource complementary discourse between “Israeli innovation” and “Korean productivity” to rationalize their partner choice as a win–win situation. CDA demonstrates how both sides are engaged in a “borrowing” process from east-to-west and head-to-hands postcolonial images to (re)produce hierarchy between the parties. While east–west mapping remained almost unchallengeable, the reversal, crossing and blurring of the Israel-to-Korea knowledge transfer direction provides a counter-narrative to resource complementarity discourse.Originality/valueThe resource complementarity discourse supported by east–west mapping and “head–hands” justifications for partner choice reveals the lingering presence of postcolonial images, imagery and imagination. By taking two nations without substantial troubled memories, histories and relations, the paper broadens the picture beyond national contexts, emphasizing the importance of borrowing and translation from postcolonial vocabulary to non-colonial situations.
The importance of the export country's environmental image for consumer responses to an imported environmentally friendly product
Authors: Thøgersen John , Pedersen Susanne 
Published: May 2021

PurposeFilling a gap in extant research regarding the measurement of an export country's environmental image and investigating its importance for consumers' evaluation of an environmentally differentiated imported product.Design/methodology/approachOnline surveys carried out in Denmark (Study 1), Germany and France (Study 2; N˜500 from each country). In Study 1, we develop an environmental country image instrument and investigate its nomological validity vis-à-vis other country image constructs and Danish consumers' evaluation of organic milk from Germany. In Study 2, we validate the instrument with consumers from Germany and France, evaluating organic milk from Denmark.FindingsConsumers differentiate between a country's environmental image and its general and production-related images. The country's environmental image is important to consumers' evaluation of an environmentally differentiated product from the country. Specifically, we find that a country's environmental image strongly influences its product-specific images and, through these, the consumer's evaluation of an organic food product from the country.Practical implicationsConsumers' use of a country's environmental image as a cue to the credibility of environmental claims gives competitive advantages to exporters from countries with a favorable environmental image, while exporters from countries with an unfavorable environmental image need measures to compensate. Companies and countries should monitor how the environmental image of their country evolves in important markets and be ready to act when facing damages to their country's environmental image.Originality/valueThis article is the first to propose a measure of environmental country image and to document that consumers use the environmental image of an exporting country to assess environmental claims on imported products.
Impact of behaviour factors on acquisition performance: mediating role of speed of post-acquisition change
Authors: Savović Slađana , Babić Verica 
Published: May 2021

PurposeThe purpose of this paper is to increase understanding of the influence of behaviour factors (corporate cultural differences and transformational leadership) on acquisition performance, through the mediating role of speed of post-acquisition change (as a process factor), in the specific context of a transitional economy.Design/methodology/approachA model was tested on a sample of acquisitions in Serbia carried out by domestic and European companies. In total, 208 valid questionnaires were collected from 10 acquired companies. Linear regression analysis was used to test the research hypotheses. To test the mediator hypothesis, Baron and Kenny's (1986) procedure was used. Statistical significance of indirect or mediated effect was calculated with Statistical Product and Service Solutions (SPSS) macro provided by Preacher and Hayes (2004).FindingsMediator analysis shows that corporate cultural differences and transformational leadership have direct and indirect impacts on acquisition performance.Practical implicationsThe results may be significant for managers involved in the processes of acquisitions, in terms of helping them to make appropriate decisions in different phases of an acquisition process, so as to obtain sufficient levels of employee commitment and trust to improve acquisition performance.Originality/valueThis research contributes to a better understanding of the relationships between behaviour factors and acquisition performance. In particular, no research into the speed of post-acquisition changes as a mediator variable between behaviour factors and acquisition performance has previously been conducted, to the best of the authors' knowledge. Thus, this research offers a unique understanding in the transitional economy context of Serbia.
Condition-based opportunistic maintenance policy for a series–parallel hybrid system with economic dependence
Authors: Jiang Aiping , Huang Zhenni , Xu Jiahui , Xu Xuemin 
Published: May 2021

PurposeThe purpose of this paper is to propose a condition-based opportunistic maintenance policy considering economic dependence for a series–parallel hybrid system with a K-out-of-N redundant structure, where a single component in series is denoted as subsystem1, and K-out-of-N redundant structure is denoted as subsystem2.Design/methodology/approachBased on the theory of Residual Useful Life (RUL), inspection points are determined, and then different maintenance actions are adopted in the purpose of minimizing the cost rate. Both perfect and imperfect maintenance actions are carried out for subsystem1. More significantly, regarding economic dependence, condition-based opportunistic maintenance is designed for the series–parallel hybrid system: preemptive maintenance for subsystem1, and both preemptive and postponed maintenance for subsystem2.FindingsThe sensitivity analysis indicates that the proposed policy outperforms two classical maintenance policies, incurring the lowest total cost rate under the context of both heterogeneous and quasi-homogeneous K-out-of-N subsystems.Practical implicationsThis model can be applied in series–parallel systems with redundant structures that are widely used in power transmission systems in electric power plants, manufacturing systems in textile factories and sewerage systems. Considering inconvenience and high cost incurred in the inspection of hybrid systems, this model helps production managers better maintain these systems.Originality/valueIn maintenance literature, much attention has been received in repairing strategies on hybrid systems with economic dependence considering preemptive maintenance. Limited work has considered postponed maintenance. However, this paper uses both condition-based preemptive and postponed maintenance on the issue of economic dependence bringing opportunities for grouping maintenance activities for a series–parallel hybrid system.
Simulation of jet printing of solder paste for surface mounted technology
Authors: Mårtensson Gustaf Eric , Göhl Johan , Mark Andreas 
Published: May 2021

Purpose
The purpose of this study is to propose a novel simulation framework and show that it captures the main effects of the deposition process, such as droplet shape, volume and speed.

Design/methodology/approach
In the framework, the time-dependent flow and the fluid-structure interaction between the suspension, the moving piston and the deflection of the jetting head is simulated. The system is modelled as a two-phase system with the surrounding air being one phase and the dense suspension the other. The non-Newtonian suspension is modelled as a mixed single phase with properties determined from material testing. The simulations were performed with two coupled in-house solvers developed at Fraunhofer-Chalmers Centre; IBOFlow, a multiphase flow solver; and LaStFEM, a large strain FEM solver. Experimental deposition was performed with a commercial jet printer and quantitative measurements were made with optical profilometry.

Findings
Jetting behaviour was shown to be affected by not only piston motion, fluid rheology and head deformation but also the viscous energy loss in the jetting head nozzle. The simulation results were compared to experimental data obtained from an industrial jetting head and found to match characteristic lengths, speed and volume within ca 10%.

Research limitations/implications
The simulations are based on a rheological description using the Carreau model that does not include a time-dependent relaxation of the fluid. This modelling approach limits the descriptive nature of the deposit after impact on the substrate. The simulation also adopts a continuum approach to the suspension, which will not accurately model the break-off of the droplet filament under the characteristic diameter of the particles in the suspension.

Practical implications
The ability to accurately simulate the deposition of functional materials will enable the efficient development of novel product designs with a minimum of used resources and minimised product development duration.

Social implications
The ability to accurately simulate the deposition of functional materials will enable the efficient development of novel product designs with a minimum of used resources and therefore an improvement from a sustainability perspective. The ability to plan deposition strategies virtually will also enable a decrease in consumables at manufacturers which will in turn decrease their carbon foot print.

Originality/value
While basic fluid dynamic simulations have been performed to simulate flow through nozzles, the ability to include both fluid-structure interaction and multiphase capability together with a more accurate rheological description of the suspension and with a substrate for surface mount applications has not been published to the knowledge of the authors.
Degree of intervention affects interannual and within‐plot heterogeneity of seed arrival in tropical forest restoration
Authors: Werden Leland K. , Holl Karen D. , Chaves‐Fallas José Miguel , Oviedo‐Brenes Federico , Rosales Juan Abel , Zahawi Rakan A. 
Published: May 2021

Abstract

In degraded tropical landscapes, lack of seed dispersal can strongly limit recovery, and restoration interventions can overcome this barrier by attracting dispersers. However, seed dispersal patterns are typically studied over short time periods, thus the influences of temporal and spatial variability on seed arrival cannot be teased apart.

The choice of management approach can have important implications for restoration‐mediated community reassembly. Accordingly, we used a 3.5‐year record of seed deposition in pre‐montane tropical wet forest in southern Costa Rica to examine how seed arrival differed between passive (natural regeneration) and active (applied nucleation, plantation) restoration after a decade of recovery, compared to remnant forest. We investigated: (a) how restoration treatments affected seed deposition rates and community composition; (b) how within‐plot heterogeneity of animal‐dispersed seed deposition varied by intervention; and (c) how interannual variation influenced animal‐dispersed seed arrival across treatments.

Overall seed rain composition and diversity in restoration treatments was converging towards, but still differed substantially from, remnant forest (89.7%, 86.6% and 76.3% Shannon diversity recovered in applied nucleation, plantation and natural regeneration respectively).

Within‐plot animal‐dispersed seed heterogeneity was similar in applied nucleation and remnant forest, 27.0% more heterogeneous in applied nucleation than plantation, and equivalent when comparing natural regeneration to either applied nucleation or plantation.

In contrast to active interventions, animal‐dispersed tree and shrub communities did not differ year to year in natural regeneration, which may promote the assembly of relatively homogeneous plant communities at this successional stage.

Synthesis and applications. Compared to natural regeneration, active restoration interventions: (a) catalysed the recovery of seed diversity (overall Shannon diversity 17.5% and 13.4% higher in applied nucleation and plantation respectively), (b) shifted seed community composition towards remnant forest more rapidly (overall Shannon diversity 13.4% and 10.2% closer), (c) almost doubled the proportion of later‐successional tree species arriving, and (d) had seed communities that differed year to year—a pattern not observed in natural regeneration. Finally, applied nucleation was the only intervention where seed arrival was as spatially heterogeneous as remnant forest, highlighting that this approach may facilitate the recovery of specific natural dispersal processes.