bluuebunny/crossref_metadata_2025_split huggingface dataset:
Individualized Medication Review in Older People: Differences Due To Place of Residence Being Their Own Home or A Nursing Home
Authors: Molist-Brunet Núria , Sevilla-Sánchez Daniel , Puigoriol-Juvanteny Emma , Bajo-Peña Lorena , Cantizano-Baldo Imma , Cabanas-Collell Laia , Espaulella-Panicot Joan 
Published: July 2021

Abstract
Background: Ageing is associated with complex and dynamic changes leading to multimorbidity and, therefore, polypharmacy. A periodic medication review in frail older people lead to optimize medication use. The aims of the study were to identify inappropriate prescription and to assess the results of a medication review in older people, according to their place of residence. Methods: This was a study with paired pre- and post-medication review data based on person-centered prescription, with a follow-up assessment at three months. We recruited patients who lived in the community, either in their own home or in a nursing home. We select patients of 65 years or more with multimorbidity whom his General Practitioner identified difficulties with the prescription management and the need of a medication review. Finally, a medication review was carried out through the application of the Patient-Centered Prescription model. Data collected were: age, sex, place of residence, morbidities, functional and cognitive status, frailty index, number of medications, therapeutical complexity, anticholinergic and/or sedative burden and monthly medication expenditure. The Chi-square test or Fisher's exact test were used to evaluate the relationship between qualitative variables and the patients’ place of residence. Student’s t-test was used to analyze the relationship between quantitative variables and the patients’ place of residence.Results: 428 patients. 90% of people presented at least one inappropriate prescription in both settings. In nursing homes, a higher number of implemented optimization proposals was detected (81.6% versus 65.7% (p<0.001)). Post-medication review, nursing-home patients had a greater decrease in their mean number of medications, polypharmacy prevalence, therapeutic complexity and in monthly medication expenditure (p<0.001). Conclusions: Patient-Centered Prescription model detected a high number of inappropriate prescriptions in both settings. However, once medication was reviewed and optimization proposals implemented, nursing-home patients presented
M6A associated TSUC7 inhibition contributed to Erlotinib Resistance in Lung Adenocarcinoma through a Notch Signaling Activation Dependent Way
Authors: Li Kai , Peng Zi-Yang , Gao Shan , Wang Qing-Shi , Wang Rui , Li Xiang , Xiao Guo-Dong , Zhang Jing , Ren Hong , Tang Shou-Ching , Sun Xin 
Published: July 2021

Abstract
BackgroundThe small tyrosine kinase inhibitors (TKIs) subversively altered the lung cancer treatments, but patients will inevitably face the therapy resistance and disease recurrence. We aim to explore the potential roles of non-coding RNAs in sensitizing the TKIs effects. MethodsMultiple cellular and molecular detections were applied to confirm the mechanistic regulations and intracellular connections. ResultsWe explored the specific gene features of candidates in association with resistance, and found the miR-146a/Notch signaling was sustained highly activated in a m6A dependent manner, and the m6A regulator of YTHDF2 suppressed TUSC7, and then contributed to the resistant features. Functionally, m6A controlled the stemness of EMT features through METTL3 and YTHDF2, and resulted in resistance in PC9ER and HCC827ER cells. The sponging regulation of TUSC7 toward to miR-146a inhibited Notch signaling functions, and affected the cancer progression and stem cells’ renewal in PC9ER and HCC827ER cells. The Notch signaling functions manipulated the cMYC and DICER inner cytoplasm, and the absence of either cMYC or DICER1 lead to TUSC7 and miR-146a decreasing respectively, formed the closed circle to maintain the balance. ConclusionPC9ER and HCC827ER cells harbored much more stem-like cells, and the resistance could be reversed by Notch signaling inactivation. The intrinsic miR-146 and TUSC7 levels are monitored by m6A effectors, miR-146 and TUSC7 themselves formed the circling loop to sustain the homeostasis. Further in clinics, the combined using of TKIs and Notch specific inhibitory non-coding RNAs will pave the way for yielding the susceptibility to targeted therapy in lung cancer.
Nomograms for predicting the overall survival of patients with cerebellar glioma: An analysis of the SEER (Surveillance Epidemiology and End Results) database
Authors: Li Jie , Huang Wobin , Chen Jiajing , Li Zhuhui , Liu Bocong , Wang Peng , Zhang Jun 
Published: July 2021

Abstract
Purpose: At present, our understanding of cerebellar glioma is still not enough. This study collected information on patients in the seer database to determine the predictive factors patients with cerebellar glioma. Patients and Methods: The data of patients with cerebellar glioma diagnosed from 1975 to 2018 were retrieved from the Surveillance Epidemiology and End Results Database. We randomly divide the data into a training group and a validation group, establish a nomogram based on the training group, and use the validation group data to verify the clinical value of the model. Results: A total of 508 patients were included in this study. Multivariate analysis was performed based on the data before randomization, and the results showed that the patient's age, the tumor's WHO grade, histological type, and extension are significantly correlated with the survival rate. The c index of OS nomograms of the training cohort was 0.909 (95% CI,(0.880-0.938）) and 0.932(95% CI, (0.889-0.975)) in validation group. The calibration curve of OS for 3 and 5 years show that there was a good consistency between the actual survival probability and the predicted survival probability.Conclusion: For patients with cerebellar glioma, the age of the patient at the time of diagnosis, WHO grade of the glioma, histological type, and extension are the four most prominent factors that affect the overall survival outcomes. Furthermore, our model may be a useful tool for predicting OS in these patients.
Prenatal Exposure to Silver is Associated with an Elevated Risk for Neural Tube Defects: A Case Control Study
Authors: Pi Xin , Wang Chengrong , Wang Di , Yin Shengju , Jin Lei , Li Zhiwen , Wang Linlin , Yue Wentao , Ren Aiguo , Yin Chenghong 
Published: July 2021

Abstract
Background: Exposure to copper, silver, and titanium has been reported to be associated with a variety of adverse effects on humans. However, few studies have focused on the fetus, which is vulnerable to environmental insults. We investigated the associations between prenatal exposure to the three metals and risk for fetal neural tube defects (NTDs).Methods: Placental samples from 408 women with pregnancies affected by NTDs and 593 women with normal pregnancies were collected from 2003 to 2016 in an NTD high-risk area in northern China. Placental metal concentrations were quantified and used as prenatal exposure markers. Multilevel mixed-effects logistic regression was used to estimate the odds ratio (OR) and 95% confidence interval (CI) for the association between metal concentrations and risk for NTDs. Single and joint effects of the metals on NTDs were evaluated with Bayesian kernel machine regression (BKMR), which can account for correlation, nonlinearity, and interaction between metals.Results: NTDs had higher concentrations of copper (4.16 μg/g) and silver (0.96 ng/g) than controls (copper: 3.91 μg/g; silver: 0.96 ng/g). Silver was associated with an increased risk for NTDs in a dose-response fashion in single-metal logistic regression, with adjusted ORs (95% CIs) of 1.78 (1.04–3.06) and 1.92 (1.11–3.32) in the second and third tertiles, respectively, compared to the lowest tertile. BKMR revealed toxic effects of silver on NTDs when the concentrations of copper and titanium were fixed at their 25th, 50th, and 75th percentiles, and the association appeared to be linear. No interaction of silver with any of the other two metals was observed. Besides, silver concentration was positively correlated with maternal certain dietary intakes, such as meat or fish, during the periconceptional period.Conclusions: High silver concentrations in placental tissue are associated with an elevated risk for NTDs in offspring. Maternal diet may be a source of silver exposure.
Case series of pectoral hematoma associated with enoxaparin use in elderly patients
Authors: Islamoglu Mehmet Sami , Cengiz Mahir , Uysal Betul Borku , Koc Suna , Unal Omer Faruk , Yavuzer Serap 
Published: July 2021

Abstract
BackgroundVenous thromboembolism is among the common and preventable cardiovascular syndromes. The risk of thromboembolism is increased in elderly and patients with renal failure, and the risk of bleeding due to the use of anticoagulants also increases. Pectoral hematoma due to enoxaparin are presented rarely unlike other bleeding localisation. In this article, 3 cases of pectoral hematoma due to enoxaparin are presented in the light of the literature, especially in this period when the use of anticoagulants is increasing.Case presentationTwo of the 3 cases over the age of 65 were in the intensive care unit, and the other patient received low molecular weight heparin therapy during outpatient follow-up. In these cases enoxaparin use for profilactic and treatment dosages. After anticoagulant using pectoral hematoma was diagnosed by ultrasonography and computed tomography of the thorax in patients. One patient was died from sepsis but in two of other with the discontinuation of the treatment, the size of the hematoma decreased and the treatment change was sufficient.ConclusionLow molecular weight heparins are used in the treatment and prevention of thromboembolism. In elderly and patients with renal failure, care should be taken in terms of pectoral bleeding and other bleeding.
Network Pharmacology and Molecular Docking Analysis of Shanhaidan granules in treatment of coronary heart disease angina pectoris
Authors: Wei Wenyang , Zheng Mengkai , Yang Yunfeng , Wang Jing , Lu Wanpeng 
Published: July 2021

Abstract
Objective

To study the pharmacological mechanism of Shanhaidan granules for treatment of coronary heart disease angina pectoris(CHD-AP).
Methods

The active ingredients and their target genes of Shanhaidan granules were collected from TCMSP and TCMID. CHD-AP-related target genes were collected from GeneCards, OMIM and DurgBank database. The target PPI network was constructed by STRING. PPI network was used for topological analysis and core target screening. The hub target proteins and top core ingredients of Shanhaidan Granule on CHD-AP was analysed for the binding validation experiment by Molecular Docking.
Results

198 active components and 320 potential targets were obtained from Shanhaidan granule, 1208 related targets and 158 joints of CHD-AP were screened. PPI network analysis showed that AKT1, IL-6, VEGF, TNF, MAPK, Caspase-3, MMP, and EGFR were the core and key teagets in treatment of CHD-AP with Shanhaidan granule. KEGG-GO analysis indicated that Shanhaidan granules treated CHD-AP by regulating the TNF signaling pathway, IL-17 signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway and apoptosis-related signalling pathway. The results of the molecular docking showed that the core compounds and target proteins had strong binding activity.
Conclusion

The results predicted and verified mechanism of Shanhaidan granule gainst CHD-AP from a holistic perspective, which provided theoretical support for rational clinical application of CHD-AP.
Identification of Prognostic Biomarkers Among DAAM1, FHOD1,FMN2 and INF2 in Breast Cancer Patients
Authors: Dai Yin-Hai , Li Fuping , Kong Wei-Jie , Zhang Xue-Qin , Wang Mao , Ma Hai-Long , Wang Qi 
Published: July 2021

Abstract
Background:The formin family proteins are main regulators of actin filaments, which play a crucial role in the migration of cells and carcinogenesis.The specific functions of the formin family proteins in breast cancer still remain unknown.To dissolve this problem,we selected four formin proteins including DAAM1,FHOD1, FMN2 and INF2 and investigated their mRNA expression and survival data in BC(breast carcinoma) patients using diverse databases.Methods:we used these databases including Oncomine, Ualcan, GEPIA 2,HumanProtein Atlas,Metascape,Kaplan-Meier plotter,cBioPortal and TIMER and the software of Cytoscape in our study.Results:DAAM1 and FMN2 were lowly expressed in BC tissues,while FHOD1 and INF2 were highly expressed in BC tissues.The expression levels of DAAM1, FMN2 and FHOD1 were relevant to major subclasses,and the mRNA level of FHOD1 was related to cancer staging.Moreover,High mRNA levels of FHOD1 and INF2 were relevant to poorer prognosis of BC patients,while low mRNA level of DAAM1 was correlated with better prognosis.we also found that there were significant associations between the expressions of DAAM1,FHOD1,FMN2 and INF2 and six types of infiltrated immune cells(B Cells,CD4+T cells,CD8+T cells, neutrophil,macrophage,and dendritic cell).Conclusions:our study indicated that FHOD1 and INF2 were potential biomarkers to identify short survival of BC patients,FMN2 was potential prognostic marker to suggest favorable survival of BC patients.
S1P/HDAC1 Signaling Regulates Macrophage Polarization in Patients with Chronic Obstructive Pulmonary Disease
Authors: Chen Yanwei , Ai Wen , Xie Peiyi , Ling Li , Gao Yulan , Wang Lei , Fang Hongcheng 
Published: July 2021

Abstract
Throughout the world, chronic obstructive pulmonary disease (COPD) is the cause of substantial morbidity and mortality and represents a large public health burden. Previous studies reported that inflammation is intimately involved in COPD, but the underlying mechanism remains unclear. As macrophage polarization was demonstrated to regulate inflammation, we hypothesized that COPD alters macrophage polarization. To test this, we investigated in individuals with COPD the relationship between S1PR1 and HADC1 expression and macrophage inflammation via flow cytometry, qRT-PCR, gene knockdown/overexpression, and western blot analysis. We found that macrophages from COPD subjects were polarized towards an M1 phenotype with increased S1PR1 and decreased HADC1experssion. S1PR1 also inhibited HADC1 expression. Thus, S1PR1/HDAC1 signaling regulates macrophage polarization. The results from this study provide insights into the pathogenesis of COPD and suggest possible therapeutic opportunities.
In Vivo Anti-Anemic Effect of an Aqueous Root Extract of Phyllanthus Muellerianus (Kuntze) Exell in Model Rats.
Authors: Nyirenda James , Lwanga Gershom B. , Muzandu Kaampwe M. , Chuba David K. , Sijumbila Gibson M. 
Published: July 2021

Abstract

Ethnopharmacological relevanceAnemia is a very serious condition in Zambia. One of the plants that has been used traditionally is Phyllanthus muellerianus where different parts of shrub are used to treat a number of diseases in Zambian folklore medicine. Earlier studies have investigated medicinal properties of its aqueous root extracts. This study evaluated the effect of P. muellerianus roots on the hematological indices of albino rats and determined its phytochemical profile. Aim of the studyTo carry out phytochemical screening of the root extract and assess the ant-anemic effect of the aqueous extract on laboratory rats with tail-bled induced anemia Materials and MethodsThirty-six male albino rats placed in six groups were used for the study. The groups comprised the 100, 200, and 400 mg/kg plant extract, Ranferon (200 mg/kg) positive control, anemic non treated control and a normal (non-anemic) control. Anemia, induced through bleeding of the rats, was defined as hemoglobin (Hb) levels less than 12 g/dL. The anti-anemic potential of the plant was determined by comparing its effect on the hematological parameters of rats on treatment to that of the control group.ResultsAfter treatment, rats on the 400 mg/kg plant extract dose showed the greatest increase in the mean values for Hb, Packed cell volume (PCV) and RBC count were 43.3±1.2%, 15.4±0.3 g/dL and 6.3±0.3 x106 /mL respectively, when compared to the negative control group (P < 0.05). Phytochemical screening revealed positive results for alkaloids, flavonoids, saponins, glycosides, steroids, triterpenoids and tannins with varying amounts.Conclusions. The aqueous root extract of P. muellerianus was efficacious against anemia in a dose-dependent manner. The phytochemical compositions seem to be responsible for its hematopoietic properties. Thus, the root decoction of P. muellerianus is useful in alleviating anemia and the results lend credence to its use in traditional medicine in the management of anemia.
Acceptability of HIV Oral Self-test Among Truck Drivers and Youths: A Qualitative Investigation From Pune, Maharashtra
Authors: Rao Amrita , Patil Sandip , Kulkarni Pranali Pramod , Devi Aheibam Sharmila , Borade Suryakant Shahu , Ujagare Dhammasagar D , Adhikary Rajatashuvra , Panda Samiran 
Published: July 2021

Abstract
INTRODUCTIONEnding AIDS by 2030 is a global target, to which India is a signatory. HIV self-test (HIVST) coupled with counselling and AIDS-care, including antiretroviral therapy, has the potential to achieve this. However, countries are at varying stages of acceptance of HIVST as discussions around introducing it in national program sparks controversy and debates. HIV self-test, as yet, is not part of the AIDS control program in India. Against this backdrop, we explored acceptability of an HIV oral self-test (HIVOST) among truckers and young men and women.METHODSA qualitative investigation with 41 in-depth-interviews and 15 group discussions was conducted among study participants in the district of Pune, in the western state of Maharashtra, India. These interactions were built around a prototype HIVOST kit, which was placed before the study participants to help taking the discussions forward. The software N-vivo (version 11.0) was used to manage the volumes of data generated through the aforementioned process. The study period spanned from June through December, 2019.RESULTSWhile the truckers belonged to the age bracket 21-67 year, the youths were in the age group 18-24 year. ‘Ease of doing HIVOST' and ‘fear of needle pricks’ were the reasons for expressing acceptance around HIVOST by both the study groups. Truckers felt that HIVOST would encourage one to know one’s HIV status and seek help as appropriate. Accuracy of HIVOST result and disposal of the kits following use were concerns of a few. Most of the participants preferred saliva over blood as the specimen of choice. Instructions in local language reportedly would enable test-use by self. The truck drivers preferred undertaking HIVOST at the truckers-friendly 'Khushi clinics’ or in the vehicle, while youths preferred the privacy of home. Some of the young men mis-perceived the utility of HIVOST by referring to doing a test on a partner immediately prior to sexual encounter. Some of the truckers had myths and misconceptions about routes of HIV transmission and treatment. CONCLUSIONSOverall, the study communities expressed their acceptance towards HIVST approach. The National AIDS Control Program, India could draw upon the findings of the current study.
UFL1 Relieves Cisplatin-Induced Premature Ovarian Failure by Reducing Endoplasmic Reticulum Stress in Granulosa Cells
Authors: Tang Xiangting , Dong Hao , Fang Zhi , Li Jingyi , Yang Qi , Yao Ting , pan zezheng 
Published: July 2021

Abstract
BackgroundUbiquitin-like modifier 1 ligating enzyme 1 (UFL1), the ligase of the Ufmylation system, has recently been reported to be involved in apoptosis and endoplasmic reticulum stress (ER stress) in a variety of diseases. Premature ovarian failure (POF) is a gynecological disease that severely reduces the fertility of women, especially in female cancer patients receiving chemotherapy drugs. Whether UFL1 is involved in the protection from chemotherapy-induced POF and its mechanism remains unclear. MethodsIn this study, we examined the function of UFL1 in ovarian dysfunction and granular cells (GCs) apoptosis induced by cisplatin through histological examination and cell viability analysis. We used western blot, quantitative real-time PCR (qPCR) and immunofluorescence (IF) to detect the expression of UFL1 and the level of ER stress specific makers. Enzyme-Linked Immunosorbent Assays were used to detect the level of Follicle-Stimulating Hormone (FSH) and Estrogen (E2) in ovaries and GCs. In addition, we knocked down or overexpressed UFL1 in ovaries or GCs through infected with lentiviral particle suspensions, respectively. ResultsOur data showed that the expression of UFL1 was reduced in POF model ovaries and was accompanied by the occurrence of ER stress. In vitro, cisplatin induced a stressful increase of UFL1 expression in GCs, and enhanced ER stress, which was aggravated by UFL1 knockdown and alleviated by UFL1 overexpression. Furthermore, the data showed that UFL1 knockdown resulted in a decrease of ovarian follicles number, an increase of atretic follicles, and a decreased expression of AMH and FSHR. Conversely, overexpression of UFL1 reduced the damage of cisplatin to the ovary in vitro. ConclusionsOur research proved that UFL1 regulates cisplatin-induced ER stress and apoptosis of GCs, and participates in the protection from cisplatin-induced POF, providing a potential therapeutic target for clinical prevention of chemotherapeutic drug-induced POF.
Patient Journey Mapping To Investigate Quality and Cultural Safety in Burn Care for Aboriginal and Torres Strait Islander Children and Families – Development, Application and Implications
Authors: Fraser Sarah , Mackean Tamara , Grant Julian , Hunter Kate , Ryder Courtney , Kelly Janet , Holland Andrew JSA , Griffin Bronwyn , Clapham Kathleen , Teague Warwick J , Darton Anne , Ivers Rebecca Q 
Published: July 2021

Abstract
Background

Quality and safety in Australian healthcare is inequitably distributed, highlighted by gaps in the provision of quality care for Aboriginal and Torres Strait Islander children. Burns have potential for long-term adverse outcomes, and quality care, including culturally safe care, is critical to recovery. This study aimed to develop and apply an Aboriginal Patient Journey Mapping (APJM) tool to investigate the quality of healthcare systems for burn care with Aboriginal and Torres Strait Islander children.
Study Design

Interface research methodology, using biomedical and cultural evidence, informed the modification of an existing APJM tool. The tool was then applied to the journey of one family accessing a paediatric tertiary burn care site. Data were collected through yarning with the family, case note review and clinician interviews. Data were analysed using Emden’s core story and thematic analysis methods. Reflexivity informed consideration of the implications of the APJM tool, including its effectiveness and efficiency in eliciting information about quality and cultural safety.
Results

Through application of a modified APJM tool, gaps in quality care for Aboriginal and Torres Strait Islander children and families were identified at the individual, service and system levels. Engagement in innovative methodology incorporating more than biomedical standards of care, uncovered critical information about the experiences of culturally safe care in complex patient journeys.
Conclusion

Based on our application of the tool, APJM can identify and evaluate specific aspects of culturally safe care as experienced by Aboriginal and Torres Strait Islander peoples and be used for quality improvement.