bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and application of a chemical profiling method for the assessment of the quality and consistency of the Pelargonium sidoides extract
Authors: Yu Se Mi , Kim Sung Jun , Yoon Yeong Cheol , Kim Jong Hoon 
Published: October 2021

AbstractThe evaluation of chemical similarity is very significant for the evaluation and standardization of the quality of herbal medicines as the biological activity of herbal medicines depends on the composition and proportion of many individual components. Many health organizations have recommended chemical profiling for the quality and consistency evaluation of herbal medicines. In this study, chemical profiling was performed to evaluate the similarity between batches of Pelargonium sidoides maltodextrin mixture (PMM) from EPs® 7630, a Pelargonium sidoides extract preparation. For chemical profile analysis, 7 common peaks were selected from 27 different PMM batches and specificity, linearity, accuracy, and precision tests were performed to develop the analytical method. The Pearson correlation coefficients of the similarity for all 27 batches manufactured over the years were higher than 0.90, indicating that quality consistency is well ensured over the years. This profiling method confirms the chemical profile of various commercial products using not only PMM but also Pelargonium sidoides extract and shows that it can be applied to standardization of quality.
FDG-PET/CT imaging for evaluating durable responses to immune check point inhibitors in patients with advanced cutaneous squamous cell carcinoma
Authors: McLean Luke S. , Cavanagh Karda , Hicks Rodney J. , Callahan Jason , Xie Jing , Cardin Anthony , Lim Annette M. , Rischin Danny 
Published: October 2021

Abstract
Background
The role of FDG-PET/CT imaging in assessing response to immunotherapy in advanced cutaneous squamous cell carcinoma (CSCC) is unknown. This study compared complete metabolic response (CMR) rates by FDG-PET and RECIST1.1 via CT or MRI in patients on cemiplimab for > 10 months.

Methods
This was a single-centre retrospective study of 15 patients treated with cemiplimab for advanced CSCC who had CT/MRI and FDG-PET/CT at > 10 months to assess metabolic treatment response. The median age was 73 years (range 55–84) and 93% were male. RECIST1.1 and PERCIST1.0 tumor responses were evaluated by blinded readers.

Results
Seventy-three percent (11/15) (95%CI 44.9, 92.2%) achieved a CMR on PET. Of these 11, on RECIST1.1 there was one complete response, 9 partial responses and one stable disease.

Conclusions
In patients on cemiplimab for > 10 months, there was discordance between CR rates on FDG-PET versus RECIST1.1. FDG-PET/CT may have utility for clarifying depth of response in patients treated with immunotherapy for CSCC.
Supporting self-management and clinic attendance in young adults with type 1 diabetes: development of the D1 Now intervention
Authors: Morrissey Eimear C. , Casey Bláthín , Hynes Lisa ,   The D1 Now Young Adult Panel, Dinneen Sean F. , Byrne Molly 
Published: October 2021

Abstract
Background
Self-management of type 1 diabetes (T1D) is complex and can be particularly challenging for young adults. This is reflected in the high blood glucose values and rates of clinic non-attendance in this group. There is a gap for a theory-based intervention informed by key stakeholder opinions to support and improve self-management in young adults with T1D.

Purpose
The aim of the work was to systematically co-develop an evidence-based and stakeholder-led intervention to support self-management and clinic engagement in young adults living with T1D in Ireland. Co-development was led by the Young Adult Panel.

Methods
The Behaviour Change Wheel was used to guide the development. Five evidence sources were used to inform the process. An iterative co-design process was used with the Young Adult Panel. Initial intervention components were refined and feasibility tested using qualitative methods.

Results
Environmental restructuring, education and training were selected as appropriate intervention functions. The co-design process, along with qualitative refinement and feasibility work, led to the final intervention content which consisted of 17 behaviour change techniques. The final D1 Now intervention consists of three components: a support worker, an agenda setting tool and an interactive messaging service.

Conclusions
The D1 Now intervention is now at pilot evaluation stage. Its transparent and systematic development will facilitate evaluation and future replications.
Additive role of dynamic subtraction MRI in assessment of unresolved HCC post-radiofrequency ablation
Authors: El-Assaly Hany , Abdallah Mohamed Farouk Hussein , Mohamed Wael Maher , Youssef Mohamed Ibrahim 
Published: October 2021

Abstract
Background
Malignant Hepatocellular carcinoma (HCC) is one amongst the foremost widespread cancers within the world. Radiofrequency ablation (RFA) is that the most generally used substitute tool for hepatic carcinoma treatment. Monitoring tumoral response to loco-regional therapy is a vital mission in oncological imaging. Dynamic contrast enhanced MR and recently added subtraction imaging technique improve assessment of the ablated hepatic focal lesions. The aim of this study is that the evaluation of the role of Subtraction MRI within the detection of recurrent or residual tumoral viability after RF ablation.

Results
Fifty patients were conducted during this retrospective study, all underwent RFA for 76 hepatic focal lesions and underwent Dynamic MRI study 1 month after ablation. Subtraction imaging was then performed. MRI images were interpreted by two readers who are experienced in hepatic imaging. The primary reader interpreted the standard Dynamic MRI and was blinded to the subtraction sequences; the second reader interpreted both Dynamic and Subtraction MRI images. the primary reader detected 49 resolved cases by dynamic MRI, while the second reader detected residual activity in 6 cases out of 49 via subtraction dynamic MRI (added value). The second reader agreed with first reader in 43 cases (agreement).The first reader detected 27 residual cases by dynamic MRI. The second reader disagreed with first reader in 11 cases appeared resolved out of 27 via subtraction dynamic MRI (added value). The second reader agreed with first reader in residual activity in 16 cases (agreement).The statistical analysis of those results revealed a big additive value of the subtraction imaging to the dynamic MRI (P < 0.001) with moderate degree of agreement between the 2 diagnostic tools (Kappa value = 0.491). This implies that Subtraction MRI significantly improves the reader confidence level within the assessment of treatment response following loco-regional therapies for HCC.

Conclusion
Dynamic Subtraction MRI is a powerful detector for real enhancement in treated HCC lesions after radiofrequency ablation and hence increasing the degree of readers’ confidence and accuracy of treated lesions in follow-up studies. So it's recommended to feature this powerful tool as a routine to any or all Dynamic MRI studies of the Liver.
Portrait and Scan
Authors: Grant Jenna 
Published: October 2021

Abstract
This article is an ethnography of color and black-and-white in medical images of a particular kind—prenatal ultrasound—in a particular place—Phnom Penh, Cambodia. It is also a meditation on histories and theorizations of color. It moves from the discourse and practice of pregnant women, family members, and doctors about color and black-and-white, to political and intellectual histories of color in Cambodia and in anthropology, to Buddhist ontologies of pregnancy and life. Across this diverse terrain, the notion of the image-affect conveys how images stimulate affective responses in viewers and how images affect their referents. A method of listening to and for image-affects helps us to understand how people relate to the elemental instability of images and the instability of beings to which images refer and with which they become.
Regulation of NANOG and SOX2 expression by activin A and a canonical WNT agonist in bovine embryonic stem cells and blastocysts
Authors: Xiao Yao , Sosa Froylan , Ross Pablo J. , Diffenderfer Kenneth E. , Hansen Peter J. 
Published: October 2021

ABSTRACT
Bovine embryonic stem cells (ESC) have features associated with the primed pluripotent state including low expression of one of the core pluripotency transcription factors, NANOG. It has been reported that NANOG expression can be upregulated in porcine ESC by treatment with activin A and the WNT agonist CHIR99021. Accordingly, it was tested whether expression of NANOG and another pluripotency factor SOX2 could be stimulated by activin A and the WNT agonist CHIR99021. Immunoreactive NANOG and SOX2 were analyzed for bovine ESC lines derived under conditions in which activin A and CHIR99021 were added singly or in combination. Activin A enhanced NANOG expression but also reduced SOX2 expression. CHIR99021 depressed expression of both NANOG and SOX2. In a second experiment, activin A enhanced blastocyst development while CHIR99021 treatment impaired blastocyst formation and reduced number of blastomeres. Activin A treatment decreased blastomeres in the blastocyst that were positive for either NANOG or SOX2 but increased those that were CDX2+ and that were GATA6+ outside the inner cell mass. CHIR99021 reduced SOX2+ and NANOG+ blastomeres without affecting the number or percent of blastomeres that were CDX2+ and GATA6+. Results indicate activation of activin A signaling stimulates NANOG expression during self-renewal of bovine ESC but suppresses cells expressing pluripotency markers in the blastocyst and increases cells expressing CDX2. Actions of activin A to promote blastocyst development may involve its role in promoting trophectoderm formation. Furthermore, results demonstrate the negative role of canonical WNT signaling in cattle for pluripotency marker expression in ESC and in formation of the inner cell mass and epiblast during embryonic development.
This article has an associated First Person interview with the first author of the paper.
The population frequency of human mitochondrial DNA variants is highly dependent upon mutational bias
Authors: Dunn Cory D. 
Published: October 2021

ABSTRACT
Next-generation sequencing can quickly reveal genetic variation potentially linked to heritable disease. As databases encompassing human variation continue to expand, rare variants have been of high interest, since the frequency of a variant is expected to be low if the genetic change leads to a loss of fitness or fecundity. However, the use of variant frequency when seeking genomic changes linked to disease remains very challenging. Here, I explored the role of selection in controlling human variant frequency using the HelixMT database, which encompasses hundreds of thousands of mitochondrial DNA (mtDNA) samples. I found that a substantial number of synonymous substitutions, which have no effect on protein sequence, were never encountered in this large study, while many other synonymous changes are found at very low frequencies. Further analyses of human and mammalian mtDNA datasets indicate that the population frequency of synonymous variants is predominantly determined by mutational biases rather than by strong selection acting upon nucleotide choice. My work has important implications that extend to the interpretation of variant frequency for non-synonymous substitutions.
An anti-tuberculosis compound screen using a zebrafish infection model identifies an aspartyl-tRNA synthetase inhibitor
Authors: Habjan Eva , Ho Vien Q. T. , Gallant James , van Stempvoort Gunny , Jim Kin Ki , Kuijl Coen , Geerke Daan P. , Bitter Wilbert , Speer Alexander 
Published: October 2021

ABSTRACT
Finding new anti-tuberculosis compounds with convincing in vivo activity is an ongoing global challenge to fight the emergence of multidrug-resistant Mycobacterium tuberculosis isolates. In this study, we exploited the medium-throughput capabilities of the zebrafish embryo infection model with Mycobacterium marinum as a surrogate for M. tuberculosis. Using a representative set of clinically established drugs, we demonstrate that this model could be predictive and selective for antibiotics that can be administered orally. We further used the zebrafish infection model to screen 240 compounds from an anti-tuberculosis hit library for their in vivo activity and identified 14 highly active compounds. One of the most active compounds was the tetracyclic compound TBA161, which was studied in more detail. Analysis of resistant mutants revealed point mutations in aspS (rv2572c), encoding an aspartyl-tRNA synthetase. The target was genetically confirmed, and molecular docking studies propose the possible binding of TBA161 in a pocket adjacent to the catalytic site. This study shows that the zebrafish infection model is suitable for rapidly identifying promising scaffolds with in vivo activity.
On-demand Patterning for Organic Light-emitting Diodes Using Laser Irradiation
Authors: Sugimoto Ryota , Morimoto Masahiro , Naka Shigeki 
Published: October 2021

Abstract
We investigated the patterning of organic light-emitting diodes using a blue laser irradiation after making a uniform device. Laser patterning was carried out under ambient atmosphere after the organic layer was deposited. The laser irradiation part was quenched, and it was shown that optional patterning was possible. This technique is expected to be a fabrication technique for on-demand pattern emission.
Η μέθοδος της Αισθητηριακής ολοκλήρωσης σε παιδιά με Αυτισμό
Authors: Χατζηστυλιανού Βασιλική 
Published: October 2021

Η σημερινή παρουσίαση εστιάζει στη μέθοδο της Αισθητηριακής Ολοκλήρωσης και στις τεχνικές παρέμβασης σε παιδιά με Αυτισμό. Ο όρος «αισθητηριακή ολοκλήρωση» άρχισε να χρησιμοποιείται τη δεκαετία του 1960, όταν η Jean Ayres έγραψε τα πρώτα επιστημονικά άρθρα αναφερόμενη στον όρο αυτόν (James, 2011). Η Α.Ο. είναι «η ικανότητα του ατόμου να συνθέτει, να οργανώνει και να επεξεργάζεται τις εισερχόμενες αισθητηριακές πληροφορίες που λαμβάνει από το σώμα και το περιβάλλον, ώστε να παράγει στοχοκατευθυνόμενες αντιδράσεις», (Arkwright, 1998). Η ίδια τόνιζε τη σημασία της ΑΟ, καθώς και τα οφέλη που έχει σε ένα παιδί με Διάχυτες Αναπτυξιακές Διαταραχές. Η μέθοδος στηρίζεται στο παιχνίδι και μέσα από αυτό, το παιδί προσλαμβάνει αισθητηριακά ερεθίσματα. Επιπλέον, αναπτύσσονται οι προσαρμοστικές αντιδράσεις, η ικανότητα επεξεργασίας και εκτέλεσης μίας δραστηριότητας, ο κινητικός σχεδιασμός, ο χρονισμός και η επίλυση προβλήματος (Ayres, 1972, Koomar & Bundy, 2002). Οι ειδικοί άρχισαν να μελετούν τα συμπτώματα του Αυτισμού και έπειτα προέβησαν σε μια σειρά επιστημονικών ερευνών ώστε να εξακριβώσουν αν όντως η μέθοδος αυτή είναι αποτελεσματική. Τα παιδιά που συμμετείχαν στις έρευνες ήταν αγόρια και κορίτσια με ΔΑΦ, ηλικίας 3-12 ετών (Fombonne, 2003). Τα αποτελέσματα έδειξαν ότι υπήρξαν θετικές αλλαγές στην αισθητηριακή επεξεργασία, στις κοινωνικές δεξιότητες, στις Δραστηριότητες Καθημερινής Ζωής, στις κινητικές δεξιότητες και στο συναισθηματικό τομέα. Κατέληξαν λοιπόν, ότι η μέθοδος δρα πολυαισθητηριακά και βοηθά στην ομαλή αυτορρύθμιση των αισθητηριακών συστημάτων του παιδιού με ΔΑΦ.
Mathieu Grenet, La fabrique communautaire: Les Grecs à Venise, Livourne et Marseille, 1770–1840
Authors: Zanou Konstantina 
Published: October 2021

Review of Mathieu Grenet. La fabrique communautaire: Les Grecs à Venise, Livourne et Marseille, 1770–1840. Athens: École française d’Athènes; Rome: École française de Rome and, 2016. 456 pp.
Dimitris Papanikolaou, Κάτι τρέχει με την οικογένεια: Έθνος, πόθος και συγγένεια την εποχή της κρίσης [There is something about the family: Nation, desire and kinship at a time of crisis]
Authors: Plantzos Dimitris 
Published: October 2021

Review of Dimitris Papanikolaou. Κάτι τρέχει με την οικογένεια: Έθνος, πόθος και συγγένεια την εποχή της κρίσης [There is something about the family: Nation, desire and kinship at a time of crisis]. Athens: Patakis, 2018. 448 pp.
Case Report: Recurrent retinal vein occlusion as the first clinical manifestation of systemic lupus erythematosus in a male patient
Authors: Ben Brahim Marwa , Arfa Sondes , Boubaker Fadia , Chelly Jihen , Ammari Wafa , Hammami Sonia , Arbi Fatma , Berriche Olfa 
Published: October 2021

Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease characterized by widespread clinical manifestations and immunological disorders. A myriad of ocular manifestations can be seen in patients with SLE. The most vision-threatening complication is vaso-occlusive retinopathy including retinal vein occlusion (RVO). RVO associated with SLE is well described in the literature and its association with antiphospholipid antibodies is recognized. However, RVO as the initial manifestation of SLE is scarcely reported. Herein, we report the first case of recurrent RVO as the revealing manifestation of SLE in a 40-year-old male patient. He had two consecutive episodes of decreased vision. Ophthalmologic examination disclosed a branch retinal vein occlusion the first time and a central retinal vein occlusion the second time. The diagnosis of SLE was established based on clinical and immunological criteria. He was prescribed antiplatelet therapy, hydroxychloroquine at 5.5 mg/kg/day, and intravitreal anti-vascular endothelial growth factor (VEGF) antibodies regimen. He slowly improved under treatment.
Learning outcomes of leader and leadership development: Method of Soft and Female leadership (SFL) empirical framework for driving the gender equality change in Mediterranean Engineering Education
Authors: Zabaniotou Anastasia 
Published: October 2021

The foundation of gender equality was built some decades ago, but higher education institutions are far from achieving it. Perhaps the leadership needs to integrate new narratives for a greater commitment especially in engineering, and new tools for the existing toolbox. This study aims to share the outcomes of soft female leadership framework (SFL) development for gender equality at the RMEI network, entailing the commitment of top-managers from engineering schools, creation of new leaders to lead the change at their institutions, students acting as change-agents, and an active community of practice. The SFL toolbox comprises self-awareness, humanistic care, intuition, creativity, and trust. The transformation of mindset, skillset, and culture entails using Sustainable Development Goals (SDGs), ‘Systemic View of Life’ arguments, and drawing knowledge from organizational learning frameworks, scientific phenomena, and mechanisms, such as a) 4I-Intuiting-Interpreting-Integrating-Institutionalizing organizational learning process starting from intuition to achieve an institutional change; b) ‘Stigmergy’ scientific mechanism of self-organized collective schemes with coordinated actions and interactions, in which the action performed by an agent leaves a trace in the environment that stimulates subsequent actions; c) ‘Spillover’ phenomenon advocating that the behavior of an agent can bring the adoption of related behaviors by other agents. The RMEI gender equality plan (GEP) was evaluated by the HORIZON2020 TARGET project consortium. The SFL excelled as successful in setting goals, articulating a policy that integrates systems approach frameworks, insights from science and technology, innovation, ecology, philosophy, self-awareness, ethics, and values. The Covid-19 pandemic disrupted physical meetings, but the process of change was not ceased at the network because we disrupted the disruption by boosting collaborative knowledge, consolidation and dissemination processes. The SFL framework integrates context, regional, and temporal characteristics, alongside cognitive, affective, and motivational outcomes over behavioral outcomes, new mindsets beyond organizational skills, and collaborative learning over individual learning.
Mission-based optimal morphing parameters for rotors with combined chord and twist morphing
Authors: Majeti Rohin Kumar , Benz Stephan 
Published: October 2021

Background: The fixed geometry rotor blades in today’s helicopters do not give the best performance throughout the duration of any mission. However, low-speed and high-speed flights have different geometrical requirements for the shape of the most efficient rotor blades. With advancements in morphing technologies, these can be applied to change the shape of the blades in different flight regimes. Methods: Two different helicopter rotor morphing concepts – namely, the linearly variable chord extension and the torque-tube based twist - under the framework of the European project SABRE were investigated for their optimal geometric parameters using a Particle Swarm Optimization (PSO) algorithm. Since the morphing parameters were dependent on the mission profile, three different missions representing typical helicopter applications were chosen. The optimization problem was posed both as single objective (power) and as multi-objective (power, tip elastic torsion and vibratory hub load). Based on the insights drawn from these investigations, a rotor was set up including both morphing concepts in a single blade. Results: The rotor with combined chord and twist morphing was shown to have better performance than the baseline blade, while keeping the penalty on the elastic torsion and vibration of the rotor to a minimum. Conclusions: Chord and twist are both important parameters determining the efficiency of a rotor blade. Since they have non-overlapping requirements, combining the two morphing concepts into a single blade can yield higher performance than the individual ones.
Identifying and characterising Thrap3, Bclaf1 and Erh interactions using cross-linking mass spectrometry
Authors: Shcherbakova Liudmila , Pardo Mercedes , Roumeliotis Theodoros , Choudhary Jyoti 
Published: October 2021

Background: Cross-linking mass spectrometry (XL-MS) is a powerful technology capable of yielding structural insights across the complex cellular protein interaction network. However, up to date most of the studies utilising XL-MS to characterise individual protein complexes’ topology have been carried out on over-expressed or recombinant proteins, which might not accurately represent native cellular conditions. Methods: We performed XL-MS using MS-cleavable crosslinker disuccinimidyl sulfoxide (DSSO) after immunoprecipitation of endogenous BRG/Brahma-associated factors (BAF) complex and co-purifying proteins. Data are available via ProteomeXchange with identifier PXD027611. Results: Although we did not detect the expected enrichment of crosslinks within the BAF complex, we identified numerous crosslinks between three co-purifying proteins, namely Thrap3, Bclaf1 and Erh. Thrap3 and Bclaf1 are mostly disordered proteins for which no 3D structure is available. The XL data allowed us to map interaction surfaces on these proteins, which overlap with the non-disordered portions of both proteins. The identified XLs are in agreement with homology-modelled structures suggesting that the interaction surfaces are globular. Conclusions: Our data shows that MS-cleavable crosslinker DSSO can be used to characterise in detail the topology and interaction surfaces of endogenous protein complexes without the need for overexpression. We demonstrate that Bclaf1, Erh and Thrap3 interact closely with each other, suggesting they might form a novel complex, hereby referred to as BET complex. This data can be exploited for modelling protein-protein docking to characterise the three-dimensional structure of the complex. Endogenous XL-MS might be challenging due to crosslinker accessibility, protein complex abundance or isolation efficiency, and require further optimisation for some complexes like the BAF complex to detect a substantial number of crosslinks.