bluuebunny/crossref_metadata_2025_split huggingface dataset:
High PON1 Expression Predicts Poor Prognosis In Kidney Renal Clear Cell Carcinoma
Authors: Jiang Chenxia , Zhang Xinyu , Li Xiaoyan , Li Jia , Huang Hua 
Published: December 2021

Abstract
Background: Relevant study had demonstrated that Paraoxonase-1 (PON1) had relationship with occurrence and development of tumors which suggested that PON1 was a key gene in promoting tumor progression. However, the relationship between PON1 and Kidney renal clear cell carcinoma (KIRC) is still unclear so far. Methods: We downloaded relevant data about KIRC from TCGA dataset and compared it with normal renal tissues. Immunohistochemistry (IHC) was applied to analyze the expression of PON1. Univariate cox regression analysis and multivariate cox regression analysis were also utilized to analyze independent factors associated with prognosis. Gene set enrichment analysis was conducted to find the signaling pathways of PON1 in KIRC. Finally, we also investigated whether PON1 had relationship with immunity. Results: As shown in results, PON1 expression was decreased in KIRC compared with adjacent paracancer tissues. Immunohistochemistry (IHC) was utilized to find the expression of PON1. After survival analysis, the high expression of PON1 was significantly related to overall survival (P<0.001). Univariate/Multivariate cox regression analysis both revealed that PON1 could serve as an independent prognostic factor. To analyze overall survival (OS) of patients with KIRC, nomogram was developed. GSEA revealed that PON1 was correlated with homologous recombination. Besides, PON1 had few relationships with immunity. Conclusions: Our results revealed that PON1 could serve as an independent prognostic factor for KIRC, providing a novel target for KIRC future treatments.
Darwin’s naturalization conundrum disentangled: the role of phylogenetic relatedness depends on the invasion stages
Authors: Omer Ali , Fristoe Trevor , Yang Qiang , Razanajatovo Mialy , Weigelt Patrick , Kreft Holger , Dawson Wayne , Dullinger Stefan , Essl Franz , Pergl Jan , Pyšek Petr , Kleunen Mark van 
Published: December 2021

Abstract
Darwin’s naturalization hypothesis predicts successful invaders to be distantly related to native species, whereas his pre-adaptation hypothesis predicts the opposite. It has been suggested that depending on the invasion stage (i.e. introduction, naturalization, and invasiveness), both hypotheses, now known as Darwin’s naturalization conundrum, could hold true. We tested this by analysing whether the likelihood of introduction for cultivation as well as subsequent stages of naturalization and invasion of species alien to Southern Africa are correlated with their phylogenetic distance to the native flora of this region. While species were more likely to be introduced for cultivation if they are distantly related to the native flora, the probability of subsequent naturalization was higher for species closely related to the native flora. Furthermore, the probability of becoming invasive was higher for naturalized species distantly related to the native flora. These results were consistent across three different metrics of phylogenetic distance. Our study reveals that the relationship between phylogenetic distance to the native flora and success of an alien species depends on the invasion stage.
Ni-Catalyzed Hydroarylation of Alkynes with Unactivated β-C(sp2)-H Bonds
Authors: ye mengchun , Qi Shao-Long , Liu Yu-Peng , Li Yi , Luan Yu-Xin 
Published: December 2021

Abstract
Hydroarylation of alkynes with unactivated C(sp2)-H bonds via chelated C-H metalation mainly occurs at γ-position to the coordinating atom of directing groups via stable 5-membered metallacycles, while β-C(sp2)-H bond-involved hydroarylation has been a formidable challenge. Herein, we used a phosphine oxide-ligated Ni-Al bimetallic catalyst to enable β-C-H bond-involved hydroarylations of alkynes via a rare 7-membered nickelacycle.
Linking Metabolites in Eight Bioactive Forage Species to Their in Vitro Methane Reduction Potential Across Several Cultivars and Harvests
Authors: Verma Supriya , Wolffram Siegfried , Salminen Juha-Pekka , Hasler Mario , Susenbeth Andreas , Blank Ralf , Taube Friedhelm , Kluß Christof , Malisch Carsten Stefan 
Published: December 2021

Abstract
An in vitro study was conducted to analyze the fermentation end-products from 17 cultivars of eight polyphenol containing forage species. The polyphenol composition and proanthocyanidin (PA) structural features of all cultivars were analyzed with UPLC-MS/MS in leaves of vegetative or generative plants. All samples were incubated with and without polyethylene glycol (PEG, a tannin-binding agent) to separate the tannin-effect on methane (CH4) production from that of the forage quality. Sulla and big trefoil, two particularly PA rich species, were found to have the highest CH4 reduction potential of up to 47%. However, they showed simultaneous and almost equal reductions in gas production (GP; a proxy for digestibility). The addition of PEG led to an increase in both GP and CH4 production, confirming the role of tannins on CH4 reduction. Moreover, PA structural features and concentration were found to be an important source of variation for CH4 production from PA containing species. Despite having low polyphenol concentrations, chicory and plantain were found to reduce CH4 production without reducing GP. Generally, the variation across cultivars from the same species was found to be lower than interspecies variability, and the results were found to be consistent across growth stages, indicating the findings representativeness.
A Study on the Effect of Feedback on the Motor Learning of Adjustability of Grasping Force in the Elderly
Authors: Yamamoto Ryohei , Akizuki Kazunori , Yamaguchi Kazuto , Yabuki Jun , Kaneno Tatsuya 
Published: December 2021

Abstract
In this study, we investigated the function related to the adjustability of grasping force (AGF) of elderly people and the effectiveness of concurrent visual feedback in learning tasks that require AGF. The young and elderly groups were measured for simple visual reaction time and a 100g AGF task that reflected the difference between desired performance and actual performance, and the main learning task was tested and practiced using concurrent visual feedback. Performance improved from pre-test to retention test in both groups, but the elderly group failed to reach the level of the young group. In addition, the retention test performance was associated with age and pre-test performance. In the elderly group, the initial performance of the practice was associated with the difference between desired performance and actual performance. In the latter half of the exercise, it was associated with visual-motor speed. The results of this study show that concurrent visual feedback is effective for learning tasks that require AGF. Indicatively, the improvement in performance during practice is insufficient, and the learning effect is reduced in elderly people who have a significant difference between desired performance and actual performance or whose visual-motor speed is slow.
Proteome Profile of Muscle Tissue of Indian Walking Catfish, Clarias Magur Exposed to Abiotic Temperature Stress
Authors: Singh Poonam Jayant , Srivast Satish Kumar 
Published: December 2021

Abstract
The study of expression of proteins in organisms on exposure to various environmental challenges gives clues for understanding on how these challenges affects and copes with the biological system. A study was undertaken to understand the proteome profile of Clarias magur, exposed to abiotic stress of water temperature, to find how fishes evolve adaptive strategies towards stress induced by unforeseen vagaries of climate change. Specimens of Clarias magur were exposed to high temperature sub-lethal water stress of 37°C for 60 days and the muscle proteome profiling was analysed through Liquid Chromatography –Mass Spectroscopy for qualitative differential profiling . The study provides an understanding of different proteins expressed as adaptative challenge to the environment. This is the first study to see proteome expression in Clarias magur through Liquid Chromatography –Mass Spectroscopy
Microfluidic System-based Time-course Tracking of Physical Proximity Between Cells and Its Effect on Gene Expression for Elucidating Live Single Cancer-immune Cell Interactions
Authors: Flores Bianca , Chawla Smriti , Ma Ning , Sanada Chad , Kujur Praveen , Chinen Ludmilla , Hukari Kyle , Lynch Mark , Ramalingam Naveen , Sengupta Debarka , Jeffrey Stefanie 
Published: December 2021

Abstract
Cell-cell communication and physical interactions play a vital role in cancer initiation, homeostasis, progression, and immune response. Here, we report a system that combines live capture of different cell types, co-incubation, time-lapse imaging, and gene expression profiling of doublets using a microfluidic integrated fluidic circuit (IFC) that enables measurement of physical distances between cells and the associated transcriptional profiles due to cell-cell interactions. The temporal variations in natural killer (NK) - triple-negative breast cancer (TNBC) cell distances were tracked and compared with terminally profiled cellular transcriptomes. The results showed the time-bound activities of regulatory modules and alluded to the existence of transcriptional memory. Our experimental and bioinformatic approaches serve as a proof of concept for interrogating live cell interactions at doublet resolution, which can be applied across different cancers and cell types.
Towards an Efficient Validation of Dynamical Whole-brain Models
Authors: Wischnewski Kevin J. , Eickhoff Simon B. , Jirsa Viktor K. , Popovych Oleksandr V. 
Published: December 2021

Abstract
Simulating the resting-state brain dynamics via mathematical whole-brain models requires an optimal selection of parameters, which determine the model’s capability to replicate empirical data. Since the parameter optimization via a grid search (GS) becomes unfeasible for high-dimensional models, we evaluate several alternative approaches to maximize the correspondence between simulated and empirical functional connectivity. A dense GS serves as a benchmark to assess the performance of four optimization schemes: Nelder-Mead Algorithm (NMA), Particle Swarm Optimization (PSO), Covariance Matrix Adaptation Evolution Strategy (CMAES) and Bayesian Optimization (BO). To compare them, we employ an ensemble of coupled phase oscillators built upon individual empirical structural connectivity of 105 healthy subjects. We determine optimal model parameters from two- and three-dimensional parameter spaces and show that the overall fitting quality of the tested methods can compete with the GS. There are, however, marked differences in the required computational resources and stability properties, which we also investigate before proposing CMAES and BO as efficient alternatives to a high-dimensional GS. For the three-dimensional case, these methods generated similar results as the GS, but within less than 6% of the computation time. Our results contribute to an efficient validation of models for personalized simulations of brain dynamics.
Primary Lymphoepithelioma-like Hepatocellular Carcinoma With Hepatic Cyst: a Case Report and Review of Literature
Authors: Yang Zhiqiang , Li Shengwei , Li Junbo , Wu Xinglang , Liu Jin , Liu Fuyao 
Published: December 2021

Abstract
Background: Lymphoepithelioma-like hepatic carcinoma is a rare malignant tumor. It includes lymphoepithelioma-like hepatocellular carcinoma (LEL-HCC) and lymphoepithelioma-like intrahepatic cholangiocarcinoma (LEL-ICC). we report the first case of hepatic cyst and LEL-HCC, which is extremely rare among hepatocellular carcinoma.Case presentation: A 47-year-old female was admitted to our hospital with an unexpected health examination finding of liver nodules. Subsequently, the Computerized tomography (CT) revealed a tumor and a cyst. The Magnetic resonance imaging (MRI) found a 23×18 mm hepatic cyst in the right lobe of the liver and a 21×17 mm nodule in the left lateral lobe of the liver, and the nodule imaging was likely liver cancer. The patient underwent laparoscopic left lateral hepatectomy, and the histopathological examination revealed undifferentiated heterogeneous glandular epithelial cells with obvious lymphocyte infiltration. The Immunohistochemistry study CD3, CD20, Ki-67, CK, and CK19 results were positive, while CD10, P53, and CEA were negative in tumor tissues. Epstein-Barr virus (EBV) was negative in situ hybridization. For this patient, the LEL-HCC diagnostic was made according to the histopathological result. Surgical resection is the first choice for this kind of patients, but immunotherapy may be more promising for them in the future. Conclusion: LEL-HCC is a rare variant of HCC, characterized by dense lymphocyte infiltration and a good prognosis. We report the first patient with hepatic cysts and LEL-HCC, which is distinctly uncommon in the HCC patients. Because the hepatic cyst and tumor are similar in certain imaging studies, our work can provide information for clinical diagnosis.
Multiscale Characterization Reveals Oligomerization Dependent Phase Separation of Primer-independent RNA Polymerase nsp8 From SARS-CoV-2
Authors: Xu Jinxin , Jiang Xin , Dong Yan , Ma Changli , Jiang Hanqiu , Zuo Taisen , Zhang Yulong , Chen Rui , Ke Yubin , Cheng He , Wang Howard , Liu Jinsong 
Published: December 2021

Abstract
RNA replication and transcription machinery is an important drug target for fighting against coronavirus. Non-structure protein nsp8 was proposed harboring primase activity. However, the RNA primer synthesis mechanism of nsp8 is still largely unknown. Here, we purified dimer and tetramer forms of SARS-CoV-2 nsp8. Combined with DLS, SANS and thermo-stability analysis, we found that both dimer and tetramer become loosened and destabilized with decreasing salt concentration, and the dimer form is more stable than the tetramer form. Further investigation showed that nsp8 dimer and tetramer can undergo phase separation but exhibit different phase separation behaviors. nsp8 dimer can form liquid-like droplets in the buffer with a low concentration of NaCl; phase separation of nsp8 tetramer depends on the assistance of RNA. Our findings on different phase separation behaviors of nsp8 dimer and tetramer could provide novel insight into the primer synthesis mechanism in coronavirus and facilitate developing novel therapeutic agents against SARS-CoV-2.
Oil Palm (Elaeis Guineensis Jacq.) Genetic Differences in Mineral Nutrition (Part III): Specific Leaflet Mineral Concentrations of High-Yielding Oil Palm Progenies and their Implications for Managing K And Mg Nutrition
Authors: DASSOU Olivier Sènankpon , Dassou Olivier Sènankpon , Adjadohoun Adolphe , Vanhove Wouter , Impens Reinout , Aholoukpè Hervé , Bonneau Xavier , Flori Albert , Cochard Bénoît , Sinsin Brice , Damme Patrick Van , Ollivier Jean 
Published: December 2021

Abstract
Background and aims: In oil palm, similar fertilization treatments can result in leaflet potassium and magnesium concentrations that vary significantly from one progeny to another. This hinders the development of standardized fertilizer recommendations for this crop, as they are usually calculated based on optimum leaflet nutrient concentrations.Methods: 4-high-yielding oil palm progenies with contrasting leaflet K and Mg concentrations (C1, C2, and C3 of Deli x La Mé origin and C4 of Deli x Yangambi origin) were treated with combinations of 3-levels of KCl and MgSO4, in a completely randomized split-plot factorial design with 6-replicates, where progenies were a sub-factor.Results: For a given level of KCl or MgSO4, different leaflet K and Mg concentrations were found between progenies (p < 0.0001). Leaflet K concentration and yield response to KCl applications revealed that the four oil palm progenies have different optimum leaflet K concentrations. In our study period (5-8 YAP), progenies C1 and C3 showed their highest fresh fruit bunch (FFB) yields (13.62 and 16.54 t ha-1 year-1, respectively) at K2, whereas progenies C2 and C4 showed their highest yields (14.62 and 12.39 t ha-1 year-1, respectively) at K1. Conclusion: Our study highlighted specific optimum leaflet K and Mg concentrations for different oil palm progenies in a given environment. It paves the way for adopting K and Mg fertilizer application rates adapted to specific requirements of each type of oil palm planting material.
Investigation on Deformation of DP600 Steel Sheets in Electric-pulse Triggered Energetic Materials Forming
Authors: Xie Xueyun , Yu HaiPing , Zhong Yang 
Published: December 2021

Abstract
Electric-pulse triggered energetic materials forming (ETEF) is a high-speed manufacturing process, which utilizes the chemical energy released by energetic materials (EMs) triggered by underwater wire discharge to plastically shape metals. The understanding of ETEF is not comprehensive, especially in the research on the discharge characteristics of energetic materials triggered by metal wires and the deformation process of metal sheets. In this paper, the above two problems were studied by means of experiment and numerical simulation. For the pulse discharge characteristics, the peak values of voltage and current were reduced during the triggering process of energetic materials. The triggering energy consumption of energetic materials was quantified to be about 200J. The matching parameters of different capacitor-voltage devices had no effect on triggering the energy release of energetic materials, so the electric pulse generator only played a triggering role on energetic materials. Compared with the quasi-static specimen with the same bulging height, the maximum major strain and thinning rate of the bulged specimen under ETEF condition were significantly reduced, and the deformation uniformity and strain distribution of the specimen were improved. The simulation results showed that the addition of energetic materials significantly improved the plastic strain energy of the blank. The deformation of the blank in ETEF can be divided into two stages: the initial chemical energy action stage and the inertia action stage. The bulging height of sheet metal increased by nearly 301% in inertia action stage, accounting for 80% of the total deformation time, and the effective plastic strain distribution was more uniform.
Development and Validation of the Iranian Body Image Scale (IBIS)
Authors: Jalali-Farahani Sara , Amiri Parisa , Zarani Fariba , Zayeri Farid , Azizi Fereidoun 
Published: December 2021

Abstract
Background: Body dissatisfaction has been known as an important public health concern which can influence physical and psycho-social health of adolescents. Hence, health professionals need a valid and reliable tool to assess this problem and its related factors in youth. This study aims to develop and assess psychometric properties of an indigenous scale for evaluation of body image and to investigate its association with body weight status among Iranian adolescents.Methods: This study conducted on 857 adolescents who completed the Iranian body image scale (IBIS) and self-reported their body weight and height. Face, content and construct validity methods were used to assess validity of the IBIS. Exploratory factor analysis (EFA) was used to assess construct validity. Moreover, internal consistency of the scale was assessed by calculating Cronbach’s alpha coefficient (α), and test-retest reliability was examined using intra-class correlation coefficient (ICC). To compare the IBIS scores across sex and body weight status groups, the Mann–Whitney and Kruskal-Wallis tests were used, respectively.Results: Mean age and body mass index (BMI) of participants were 16.5±1.1 years and 22.4±4.5 kg/m2, respectively. The EFA showed a 10-factor construct which explained 57% of the variance. The Cronbach’s alpha coefficient for overall items of the scale was 0.896 and ranged from 0.42 to 0.92 for subscales. The test retest reliability result was acceptable for the IBIS (ICC= 0.805). The ICC values ranged from 0.753 to 0.990 for IBIS subscales. The IBIS total score was significantly higher in girls compared to boys (median (Q1-Q3): 32.2 (25.5-43.3) vs. 30.3(24.0-38.0); p<0.001). In girls, the IBIS total score was significantly lower in normal weight girls compared to their overweight (p=0.001), and obese (p=0.001) counterparts. This difference was not observed in boys. Conclusion: These findings support reliability and validity of the IBIS for assessment of body image in Iranian adolescents; this scale can be applied as an appropriate tool for assessment of body image in adolescents in related future studies. Current findings highlight considering body image as an important aspect of intervention programs targeting overweight and obesity in adolescents, specifically girls.
Effect of High-Pressure Hot Airflow On Interlayer Adhesion Strength of 3D Printed Parts
Authors: Xu Huangxiang , Xiao Jianhua , Zhang Xiaojie , Liu Xiaobo 
Published: December 2021

Abstract
The characteristics of FDM 3D printed parts depend largely on the process used to make them. This paper demonstrates the design of an FDM 3D printing gas-assisted molding printing head, which is used to eliminate the effect of swelling away from the mold and improve the dimensional accuracy. Meanwhile, the high-pressure hot airflow instantly heats and pressurizes the printing surface layer to enhance the interlayer adhesion strength and its mechanical properties. A stable gas lubrication layer can be formed on the inner wall of gas-assisted nozzle to smoothly deposit filaments when the gas flow (Qgas) is set to 1.75 L/min and the gas pressure (Pgas) is set to 0.4 MPa. The interlayer adhesion strength of the printed parts is enhanced by more than 50% compared with that without gas assistance, and the volumetric shrinkage rate of the optimal group is only 0.13%. The proposed printing method can significantly improve the performance of thermoplastic parts and provide new capabilities for biomedical printing, automotive, aerospace and functional device printing in the future.
Endogenous Levels of Alpha-synuclein Modulate Seeding and Aggregation in Cultured Cells
Authors: Vasili Eftychia , Dominguez-Meijide Antonio , Flores-Léon Manuel , Al-Azzani Mohammed , Kanellidi Angeliki , Melki Ronald , Stefanis Leonidas , Outeiro Tiago 
Published: December 2021

Abstract
Background: Parkinson's disease is a progressive neurodegenerative disorder characterized by the accumulation of misfolded alpha-synuclein in intraneuronal inclusions known as Lewy bodies and Lewy neurites. Multiple studies strongly implicate the levels of alpha-synuclein as a major risk factor for the onset and progression of Parkinson’s disease. Alpha-synuclein pathology spreads progressively throughout interconnected brain regions but the precise molecular mechanisms underlying the seeding of alpha-synuclein aggregation are still unclear.Methods: Here, using stable cell lines expressing alpha-synuclein, we examined the correlation between endogenous alpha-synuclein levels and the seeding propensity by exogenous alpha-synuclein pre-formed fibrils. We applied biochemical approaches and imaging methods in stable cell lines expressing alpha-synuclein and in primary neurons to determine the impact of alpha-synuclein levels on seeding and aggregation. Results: Our results indicate that the levels of alpha-synuclein define the pattern and severity of aggregation and the extent of p-alpha-synuclein deposition, likely explaining the selective vulnerability of different cell types in synucleinopathies. Conclusions: The elucidation of the cellular processes involved in the pathological aggregation of alpha-synuclein will enable the identification of novel targets and the development of therapeutic strategies for Parkinson's disease and other synucleinopathies.