bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Photobiomodulation/Laser Therapy Combined With Resistance Training on Quadriceps Hypertrophy and Strength, and Postural Balance in Older Women: A Randomized, Triple-Blinded, Placebo-Controlled Study
Authors: Rodrigues Claudiane Pedro , Jacinto Jeferson Lucas , Roveratti Mirela Casonato , Merlo Jeanne Karlette , Soares-Caldeira Lúcio Flávio , Silva Ribeiro Alex , Nunes João Pedro , Junior Eros de Oliveira , Aguiar Andreo Fernando 
Published: May 2021

Background and Purpose:
Physical and therapeutic strategies to maintain and rehabilitate skeletal muscle mass, strength, and postural balance are clinically relevant to improve the health, well-being, and quality of life of older adults. The purpose of this study was to investigate the effects of photobiomodulation (PBM)/laser therapy combined with a resistance training (RT) program on quadriceps hypertrophy and strength, and postural balance in older women.

Methods:
In a randomized, triple-blinded, placebo-controlled design, twenty-two older women (age 66.6 ± 5.2 years) were engaged in a supervised 10-wk RT program (2 times per week) involving unilateral leg extension exercise, in which each leg of the same participant was randomly assigned to receive active (λ = 808 nm, optical output = 100 mW, total energy = 42 J) or placebo laser PBM immediately before the RT sessions. Maximal dynamic strength by unilateral knee extension 1-repetition maximum (1RM), muscle hypertrophy by vastus lateralis muscle thickness, and postural balance by one-legged stance test on a force platform were assessed before and after the training program.

Results:
Significance statistical analysis revealed a similar improvement (time P = .003) from pre- to posttraining for muscle hypertrophy and strength, and postural balance between active and placebo laser conditions. However, clinical interpretation for muscle hypertrophy showed a moderate effect (effect size [ES] = 0.58) for the active laser and a small effect (ES = 0.38) for the placebo laser. Clinical difference was not noticed between conditions for other analyzed variables.

Conclusions:
These findings indicate that RT alone can be clinically important for counteracting the deleterious effects of aging on muscle size, strength, and balance, and that applying laser PBM therapy before the RT sessions may further improve gains in muscle hypertrophy.
Estrategias y Mecanismos para la Construcción de una Cultura de Paz en la Educación Secundaria en Bogotá, Colombia
Authors: Acosta Oidor Catalina , Tabares Rojas Luz Ángela , Castillo Acosta Paula Natalia , López Andrade María Catalina , Luque Ramírez Luisa Fernanda , Ortiz Arévalo Ana María , Vargas Rodríguez Nataly 
Published: May 2021

El presente artículo es resultado de una investigación sobre los mecanismos y estrategias promovidos en la educación secundaria para generar una cultura de paz en Colombia, una sociedad que busca transformar un contexto generalizado de conflicto armado en un ambiente donde primen las soluciones negociadas. Esta intención se manifiesta a través de la firma del acuerdo de paz en 2016, entre el gobierno de 2010 -2018 y la guerrilla FARC. La sociedad experimentó desde la segunda mitad del siglo XX las consecuencias de este conflicto, evidenciadas en la priorización de la violencia como mecanismo para gestionar las diferencias. Por esta razón, se hace necesario evidenciar las formas en las que se prepara a la sociedad para un ambiente pacífico. Uno de los escenarios principales para ello es sin duda el sistema educativo –escuelas, colegios, universidades-. En el presente caso se estudia la experiencia de dos colegios: IED Antonio José Uribe y Santa Francisca Romana de Bogotá. A través de una metodología mixta que involucró encuestas, entrevistas y talleres, se evidenció cómo la paz y la violencia son dos ejes presentes en la vida cotidiana y no necesariamente se restringen a consideraciones sobre lo que ocurre a nivel político.
CЕЗОННІ ЗМІНИ ТАКСОНОМІЧНОЇ СТРУКТУРИ ТА ЧИСЕЛЬНОСТІ СТАФІЛІНІД (COLEOPTERA, STAPHYLINIDAE) ГЕРПЕТОБІЮ УРБОЦЕНОЗІВ МІСТА ХАРКІВ (УКРАЇНА)
Authors: Putchkov A. V. , Komaromi N. A. , Batschynskaja J. A. 
Published: May 2021

Всього в урбоценозах Харкова зареєстровано 66 видів стафілінід з 37 родів. Staphylinus caesareus та Drusilla canaliculata відзначені як еудомінанти, а 15 видів виявилися домінантами чи субдомінантами в більшості біотопів. Зростання числа видів відзначено з кінця квітня (12–15), і сягає максимуму в травні-червні (28–32 види). У липні– серпні відзначено по 10 та 16 видів відповідно, а восени – до 9 видів. Висока активність жуків характеризувалась одним–двома піками відзначеними з квітня до середини липня (але з максимумом в кінці травня). С другої половини літа чисельність стафілінід знижувалась, і досягала мінімуму восени. Перше збільшення чисельності жуків (кінець квітня-травень), обумовлено масовим виходом жуків, що перезимували. Другий пік чисельності (кінець червня-середина липня чи серпень) пов'язано як з появою жуків нового покоління, так і міграціями ряду видів стафілінід (особливо в літній період). Розглянуті сезонні зміни чисельності стафілінід у різних урбоценозах: парках, лісопарку, насадженнях центру та околиць міста і на присадибних ділянках. Висока чисельність стафілінід в центрі міста відзначена з квітня-травня (максимум) до початку червня. У парках та в Лісопарку, пік активності стафілінід спостерігали з другої половини квітня до середини травня, а значне зниження –з кінця червня до початку-середини серпня. Максимум чисельності Staphylinidae в насадженнях околиць міста характеризувалося одним-двома пікамим – з середини травня до червня та в першій половині червня, а різке зниження відзначено з середини літа. На присадибних ділянках пік чисельності в спостерігали у другій половині травня (2018) та у другій половині квітня (2019), після чого активність Staphylinidae істотно знижувалась. Можна припустити, що підвищення чисельності стафілінід весною (кінець квітня-травень) у всіх біотопах обумовлено виходом, активним пошуком їжі і розмноженням особин, що перезимували (в першу чергу Staphylinus caesareus), а в ряді випадків (в середині червня) і появою жуків нового покоління (наприклад, для Drusilla canaliculata). В подальшому, зниження активності, обумовлено смертністю більшості жуків після відкладання яєць, міграціями в інші біотопи в зв'язку з погіршенням умов, наприклад, низькою вологості ґрунту і підвищенням температури влітку. Максимальні показники чисельності (на прикладі S. саesareus) навесні зареєстровано на півтора-два місяці раніше, ніж такі статевого індексу. Максимальна частка самиць відзначена з другої половини літа і до початку осені.
Effect of Ferula elaeochytris on irisin levels in rat heart
Authors: ESER Nadire , TÜRK Ahmet , KARADAĞ Abdullah , YOLDAŞ Atila 
Published: May 2021

Purpose: The aim of this study was to determine the levels of irisin in the heart, which is a vital organ, in rats of different ages and to reveal whether Ferula elaeochytris (FE) has an effect on the expression of the irisin.
Materials and Methods: Our study was designed in six groups, young (4 months), middle age (12 months), old (24 months) rats which were given FE extract for 8 weeks and their control groups. After that their heart tissues were taken and irisin levels were measured by immunohistochemical staining method.
Results: The obtained results were analyzed by performing histoscoring. It was observed that the level of irisin in old rats was statistically significantly lower in young and middle age rats. In addition, irisin level of in all groups administered with FE extract was found significantly increased.
Conclusion: FE extract increases the level of irisin through other pathways besides its protective effect against tissue damage.
APPLICATION OF MULTIPOTENT MESENCHYMAL STEM CELL SECRETOME IN THE TREATMENT OF ADJUVANT ARTHRITIS AND CONTACT-ALLERGIC DERMATITIS IN ANIMAL MODELS
Authors: Golubinskaya P. A. , Sarycheva M. V. , Dolzhikov A. A. , Bondarev V. P. , Stefanova M. S. , Soldatov V. O. , Nadezhdin S. V. , Korokin M. V. , Pokrovsky M. V. , Burda Yu. E. 
Published: May 2021

The therapeutic effect of multipotent mesenchymal stem cells has been proven on various disease models. One of the mechanisms is the paracrine effect of the cells on the surrounding tissues.The aim. To investigate the secretome effectiveness of the multipotent mesenchymal stem cells in the treatment of adjuvant arthritis and contact-allergic dermatitis in Wistar rats.Materials and methods.Adjuvant arthritis was simulated in 26 female rats by the administration of Freund's complete adjuvant and then treated with the administration of 100 µl of multipotent mesenchymal stem cell secretome or saline. Contact-allergic dermatitis was modeled on 30 female rats by applying 200 μl of an oil solution of dinitrofluorobenzene to the skin on days 1, 5 and 6. Then the rats were treated with fluocinolone ointment (a positive control), baby cream (a negative control), baby cream with a secretome of native multipotent mesenchymal stem cells or from the cells processed with dexamethasone.Results.Judging by the indicators of the longitudinal and transverse dimensions of the paws in rats and a histological examination, the secretome did not have any anti-inflammatory effect on adjuvant arthritis. A cream with a secret from multipotent mesenchymal stem cells processed with dexamethasone, was the most effective on the model of contact-allergic dermatitis: the clinical improvement occurred on the 2ndday. The secretome from native multipotent mesenchymal stem cells and fluocinolone had a therapeutic effect on the 3rdday of application, the negative control - on the 4thday. The lymphocytic infiltration coefficient was significantly lower (p <0.05) in all the cases compared to the negative control (2.8 ± 0.1). However, the lowest infiltration was observed when the cream with secretome from native (1.75 ± 0,1) and dexamethasone-stimulated (1.76 ± 0.1) multipotent mesenchymal stem cells was being used.Conclusion.The cream with the secretome of multipotent mesenchymal stem cells suppresses lymphocytic infiltration more strongly than the highly active topical glucocorticosteroid - fluocinolone - on the model of contact-allergic dermatitis, which is a classic local delayed-type hypersensitivity reaction. However, a further study of the therapeutic effect of the secretome on models of systemic inflammatory diseases is required after its preliminary purification from large-molecular proteins.
STUDY OF PHARMACEUTICAL SPECIALISTS’ INFORMATION AWARENESS ON THE MATTERS OF DRUG ABUSE
Authors: Porseva N. Yu. , Soloninina A. V. , Dvorskaya O. N. 
Published: May 2021

Prevention of non-medical use of drugs is the most important task of the state anti-drug policy of the Russian Federation, the effectiveness of which can largely be due to the professional awareness of pharmaceutical specialists, their compliance with the established regulations for the dispensing of drugs, and proper pharmaceutical advice.The aim. The research of pharmaceutical specialists’ information awareness on the matters of drug abuse.Material and methods. The study was based on the analysis of the regulatory legal acts of the Russian Federation governing the procedure for prescribing and dispensing drugs, instructions for the medical use of the drugs used for the purpose of abuse. In the course of the study, a systematic approach has been applied. It includes methods of structural-logical, cluster and content analyses, methods of generalization and grouping. The study of pharmaceutical specialists’ information awareness on the matters of drug abuse was carried out using a random sample survey using a specially developed questionnaire: 396 employees of pharmacy organizations of various forms of property from the Perm Territory, the Chelyabinsk and Kirov regions, the Udmurt and Chuvash Republics, the Komi Republic, were questioned in the period from 2017 to 2019. The questionnaire included 35 questions, structured in 4 blocks. The first block included questions on education, position, work experience of the respondents, the second - questions on identifying knowledge on the range of drugs used for abuse, and categories of consumers of such drugs. The third block contained questions on the regulation and compliance with the procedure for dispensing drugs. The fourth block of the questionnaire was intended to establish the methods and sources of obtaining information on the abuse of the drugs sold by specialists of pharmacy organizations.Results. On the basis of the analysis carried out, an insufficient level of knowledge by specialists of the regulatory legal acts governing the trade of drugs, the range of drugs used for the purpose of abuse, and the categories of their consumers have been established. The violation of the rules for dispensing drugs has been revealed, as well as the lack of systematic sources of information on drug abuse.Conclusion. The need to develop a training program for pharmaceutical specialists on the prophylaxis and prevention of drug abuse has been established. In order to work out additional competencies in the prevention of non-medical use of drugs and improve the quality of pharmaceutical consulting, it is necessary to conduct appropriate educational activities.
Analisis Kestabilan dan Kontrol Optimal Model Matematika Partisipasi Pemilih pada Pemilihan Umum dengan Saturated Incidence Rate
Authors: Putri Dinda Ariska , Windarto Windarto , Alfiniyah Cicik 
Published: May 2021

Voter participation in general elections is an important aspect of a democratic state structure. Participation is determined by the level of public political awareness, if the level of public political awareness is low, voter participation tends to be passive (Abstinence). A mathematical model approach to voter participation in elections that has been modified to a saturated incidence rate is needed to predict voter participation in future elections. This thesis aims to analyze the stability of the equilibrium point and apply the optimal control variable in the form of an awareness campaign. In the model without control variables, we obtain two equilibriums, namely, the non-endemic equilibrium and the endemic equilibrium. Local stability and the existence of endemic equilibrium depend on the basic reproduction number (R0), where R0=bL/(g+m)m. There is voter participation in elections when R0 < 1 and the absence of voter participation in elections when R0 > 1. We also analyze the sensitivity of parameters to determine which parameters are the most influential in this mathematical model. Furthermore, the application of control variables in the mathematical model of voter participation in elections with saturated incidence rate is determined through the Pontryagin Maximum Principle method. Numerical simulation results show that providing control variables in the form of awareness campaign it is quite effective in minimize the number of the voting population who abstained from election.
Special Issue on e-ASIA JRP: Development of a Landslide Monitoring and Prediction System in Monsoon Asia
Authors: Wakai Akihiko , Sato Go , Tran The Viet , Karnjana Jessada , Komori Jiro 
Published: May 2021

This special issue summarizes some of the findings of the first half of our international joint research between Japan, Thailand, and Vietnam. This collaborative research is based on the framework of the e-ASIA Joint Research Program (e-ASIA JRP) and lasts for three years. Rainfall-induced landslides are a common disaster in many Asian countries. Our goal is to develop a practical method for landslide susceptibility mapping so that there are fewer landslide disasters in the future.
The e-ASIA JRP is an international joint initiative of public funding organizations in the East Asia Summit member countries. Based on the co-funding mechanism, support for the research teams is received from the funding organizations in their respective countries. Since 2019, the Japanese, Thai, and Vietnamese teams have been supported by the Japan Science and Technology Agency (JST), the National Science and Technology Development Agency of Thailand (NSTDA), and the Ministry of Science and Technology of Vietnam (MOST), respectively.
In the first half of our project, we completed the basic steps for developing the system. In this special issue, we are proud to present some of our achievements, including studies on slope failure analysis, landslide prevention works, meteorological observations, landslide monitoring, statistical or wide-area risk evaluations, mathematical models, and flash flood control. In addition to the above, we also present other valuable research achievements that related members have provided to help ensure the achievement of our goals. In total, 20 papers are published here. We believe that our comprehensive research activities will dramatically increase future landslide disaster mitigation, especially in monsoon Asia, and will strongly augment the roadmap for achieving the global Sustainable Development Goals (SDGs) as a common desire of humanity.
Transfusion of rare cryopreserved red blood cell units stored at –80°C: the French experience
Authors: Peyrard T. , Pham B.N. , Le Pennec P.Y. , Rouger P. 
Published: May 2021

Abstract
The technology allowing freezing of RBC units has been available for many decades. The high-glycerol method for RBC storage at –80°C is predominantly used. Several studies have shown satisfactory results regarding the in vitro viability and function of cryopreserved RBCs. RBC freezing is nowadays mostly encountered in rare blood programs and military deployments. Preservation time of frozen RBCs appears to be virtually indefinite, but most countries apply a 10-year outdate. There is no mandatory time restriction in France. The National Rare Blood Bank currently includes 962 (17.5%) RBC units aged 10 years or more and 153 (2.8%) aged 20 years or more. Since 1994, 1957 RBC units have been thawed and transfused, among which 118 were aged 10 years or more and 8 were aged 20 years or more. Discarding RBC units older than 10 years may be highly sensitive for very rare blood groups, e.g., U–, of which approximately 30 percent of the cryopreserved units are aged 10 years or more. However, the lack of nucleic acid testing for HIV and HCV may be problematic for old RBC units drawn from donors who were not subsequently tested for these markers, which is now mandatory in most countries. Regarding the 118 transfused RBC units older than 10 years, no evidence of hemolysis of thawed RBCs and no transfusion reaction, clinical or biologic hemolysis, or transfusion ineffectiveness was reported, either by any of the parties involved in the transfusion supply of rare RBC units or through the French hemovigilance program, which requires a mandatory report of any transfusion reaction. It has recently been suggested to extend the 10-year restriction in some countries. Considering our experience and observational data, we may consider it safe and efficient to transfuse rare frozen RBC units older than 10 years. An international consensus for RBC cryopreservation time should ideally be established.
Immunohematology

2009;25:13–17.
Transfusion of multiple units of Js(b+) red blood cells in the presence of anti-Jsb in a patient with sickle β-thalassemia disease and a review of the literature
Authors: Yuan S. , Ewing N.P. , Bailey D. , Salvador M. , Wang S. 
Published: May 2021

Abstract
Jsb is a high-frequency antigen. Anti-Jsb is a rare alloantibody, and its clinical significance is poorly documented. We report a case in which a 12-year-old boy of Nigerian descent with sickle β-thalassemia presented with multiple alloantibodies, including a panagglutinin and acute chest syndrome, necessitating the emergent transfusion of five units of phenotype-similar, crossmatch-incompatible RBCs, four of which were given during an exchange transfusion. The patient was later found to have anti-Jsb. In addition to routine serologic methods to study the patient’s RBCs and plasma, a monocyte monolayer assay (MMA) was performed on the patient’s samples obtained 2 and 9 days after transfusion of the Js(b+) RBCs to determine the potential clinical significance of the anti-Jsb. Various laboratory parameters including quantitative hemoglobin fraction analyses were used to monitor for increased RBC destruction. The MMA reactivity of the patient’s anti-Jsb increased from 2.3 percent on day 2 after transfusion to strongly positive at 88 percent and 66.5 percent (with and without the addition of fresh serum) 1 week later. MMA reactivity of greater than 5 percent is associated with increased RBC destruction. There was no clinical or laboratory evidence of increased hemolysis above baseline. However, decreased RBC survival was suggested by the relatively brisk decrease of the HbA1 fraction after the transfusions. The current case and others reported in the literature suggest that anti-Jsb may have limited potential for causing overt hemolysis. However, in patients with underlying hematologic disease, even mildly increased RBC destruction may pose problems clinically, and thus transfusion of Js(b+) RBCs should be avoided. In emergent situations, the potential of adverse effects of transfusing incompatible units should be balanced against the risk of withholding transfusion. Family members, especially siblings, should be considered as potential designated donors for patients with antibodies directed against high-frequency antigens. Available reports on anti-Jsb in the literature are also reviewed.
Immunohematology

2007;23:75–80.
A single base insertion of the 4-α-galactosyltransferase gene led to the deficiency of Gb3 biosynthesis
Authors: Tanaka M. , Yamashita N. , Takahashi J. , Hirayama F. , Tani Y. , Shibata H. 
Published: May 2021

Abstract
cDNAs for α 1,4 galactosyltransferase (A4GALT) have been isolated. To explore the molecular basis of the p phenotype in Japanese donors, we analyzed the A4GALT gene sequences of normal and p phenotype samples. The coding region in the A4GALT gene for DNA sequencing was amplified by PCR amplification. A4GALT expression vectors for individual mutants were constructed by PCR amplification of the coding region using primers and subsequent subcloning into an expression vector. The expression of Gb3/CD77 antigen on the cell surface was evaluated by flow cytometry and by immunochemical techniques. All individuals with the p phenotype were found to have a single base insertion (A4GALT/insC) at the same nucleotide position. Neither the transfectant cells with a mutant gene (A4GALT/insC) of donor origin or those with a synthesized mutant gene (A4GALT/insC-Mu) expressed Gb3 antigen indicating that the presence of A4GALT/insC diminished the A4GALT enzyme activity. In addition, an allele-specific PCR (ASP) system was developed in which donors of the p phenotype with A4GALT/insC can be unambiguously discriminated from normal donors. Based on the finding that a single base insertion (A4GALT/insC) diminishes A4GALT activity, an ASP assay was developed to detect individuals with this particular p phenotype.
Immunohematology

2006;22:23–29.