bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pelatihan Pembuatan Pupuk Organik Padat dari Limbah Pertanian di Kelompok Tani Sadar Kelurahan Limbukan guna Mendukung Pertanian Berkelanjutan
Authors: Sari Deliana Andam , Yanti Rinda , Ibrahim Hasan , Dharma Setya 
Published: December 2024

Permasalahan utama kelompok tani di wilayah Limbukan adalah penggunaan pupuk anorganik secara terus menerus dan minimnya aplikasi pupuk organik di lahan pertanian. Petani belum memahami manfaat dan cara mengembalikan bahan organic ke lahan pertanian. Pemanfaatan limbah pertanian dari hasil panen, limbah kotoran masih belum optimal dilakukan oleh petani. Metode yang digunakan pada kegiatan pengabdian Masyarakat adalah difusi Ipteks dan pelatihan kepada kelompok tani Sadar mengenai pembuatan pupuk organik padat, demonstrasi/ pelatihan . Kegiatan pengabdian telah dilakukan pada bulan April 2024 Hingga September 2024. Dari hasil pengabdian yang telah dilakukan, petani sangat antusias untuk mengadopsi teknologi yang telah di berikan selama kegiatan berlangsung. Petani langsung menerapkan produk pupuk organic padat yang dihasilkan ke tanaman jagung. Penggunaan pupuk organic padat diaplikasikan ke lahan tanam1 minggu sebelum tanam.
Construction and clinical application of a risk model based on N6-methyladenosine regulators for colorectal cancer
Authors: Zhu Hanhan , Yang Yu , Zhou Zhenfeng 
Published: December 2024

Background
Colorectal cancer (CRC) shows a high incidence in developed countries. This study established a prognosis signature based on N6-methyladenosine (m6A) regulators involved in CRC progression.

Method
The bulk RNA-seq data from the Atlas and Compass of Immune-Colon cancer-Microbiome interactions (AC-ICAM) and GSE33113 CRC datasets were obtained from the cBioportal and GEO databases, and a total of 21 m6A regulators genes were collected from a previous study. The scRNA-seq analysis of the GSE146771 cohort was conducted applying the Seurat and harmony R packages. Consensus clustering based on the expressions of m6A regulators was performed with the ConsensusClusterPlus package. The ggGSEA package was used for the Gene Set Enrichment Analysis (GSEA). The un/multivariate and LASSO Cox analysis were performed applying the “survival” and “glmnet” packages for developing a risk model. The pRRophetic and GSVA packages were utilized to analyze potential drugs for CRC and immune infiltration in different risk groups, followed by the Kaplan–Meier (KM) survival and ROC analysis with the “survival” and “timeROC” packages. In vitro assays included the quantitative polymerase chain reaction (qPCR), wound healing and transwell were performed.

Results
CRC patients in the AC-ICAM cohort were assigned into three molecular subtypes (S1, 2 and 3) based on nine m6A regulator genes. Specifically, the prognostic outcome of the S3 was the most favorable, while that of the S1 was the worst and this subtype was associated with the activation of NF-kB, TNF-α and hypoxia pathways. Three key genes, namely, methyltransferase-like 3 (METTL3), insulinolike Growth Factor2 mRNA-Binding Protein 3 (IGF2BP3) and YTH domain-containing protein 2 (YTHDC2), selected from the 9 m6A regulator genes were combined into a RiskScore, which showed a high classification effectiveness in dividing the patients into high- and low-risk groups. Inhibition of the expression of METTL3A or that of IGF2BP3 suppressed the invasion and migration of CRC cells. Notably, the high-risk patients had higher immune cell infiltration to support the activation of multiple immune responses and exhibited significantly poor prognosis. Meanwhile, a nomogram with practical clinical value was developed based on the RiskScore and other clinical features. Finally, eight potential drugs associated with the RiskScore were identified, and CD4+ cells and Tregs were found to be closely associated with CRC progression.

Conclusion
The RiskScore model developed based on m6A regulators played a critical role in CRC development and can be considered as a prognosis predictor for patients with the cancer. The present discoveries will facilitate the diagnosis and clinical management of CRC patients.
Prolonged sedentary time under the state of emergency during the first wave of coronavirus disease 2019: Assessing the impact of work environment in Japan
Authors: Koyama Teruhide , Takeuchi Kenji , Tamada Yudai , Aida Jun , Koyama Shihoko , Matsuyama Yusuke , Tabuchi Takahiro 
Published: August 2021

Abstract

Objectives
The coronavirus disease 2019 (COVID-19) outbreak and the resulting state of emergency have restricted work environments, which may contribute to increased duration of sedentary behaviors. This study investigated the self-reported sedentary time of Japanese workers during and after the first state of emergency (April 7 to May 25, 2020) and examined differences in sedentary time after starting work from home and according to job type.

Methods
We used cross-sectional data from the Japan COVID-19 and Society Internet Survey, a web-based questionnaire survey conducted from August to September 2020 (n = 11,623; age range 15-79 years; 63.6% male). Prolonged sedentary time was calculated by subtracting the sedentary time after the state of emergency (defined as the normal sedentary time) from that during the emergency, with adjustments using inverse probability weighting for being a respondent in an internet survey.

Results
An increase in sedentary time of at least 2 hours was reported by 12.8% of respondents who started working from home during the state of emergency, including 9.7% of salespersons and 7.7% of desk workers. After adjusting for potential confounders, the multivariate-adjusted odds ratios (ORs) for a prolonged sedentary time ≥2 hours was significantly higher in respondents who started to work from home (OR: 2.14, 95% confidence interval: 1.78-2.57), and certain job types (desk workers; OR: 1.56, 95% CI: 1.27-1.91, salespersons; OR: 2.03, 95% CI: 1.64-2.51).

Conclusions
Working from home and non-physical work environments might be important predictors of prolonged sedentary time.
In–Situ‐Sprayed Dual‐Functional Immunotherapeutic Gel for Colorectal Cancer Postsurgical Treatment
Authors: Si Xinghui , Ji Guofeng , Ma Sheng , Xu Yudi , Zhao Jiayu , Zhang Yu , Huang Zichao , Tang Zhaohui , Song Wantong , Chen Xuesi 
Published: August 2021

AbstractSurgery remains the most preferred treatment options for colorectal cancer (CRC). Paradoxically, local recurrence and distant metastasis are usually accelerated postsurgery as a consequence of local and systemic immunosuppression caused by surgery. Therefore, modulating tumor postoperative immune microenvironment and activating systemic antitumor immunity are necessary supplementaries for CRC therapy. Here, an in‐situ‐sprayed immunotherapeutic gel loaded with anti‐OX40 antibody (iSGels@aOX40) is reported for CRC postsurgical treatment. The iSGel is formed instantly after spraying with strong adhesion ability via crosslinking between tannic acid (TA) and poly(l‐glutamic acid)‐g‐methoxy poly(ethylene glycol)/phenyl boronic acid (PLG‐g‐mPEG/PBA). TA not only serves as one component of the iSGel but also relieves the postsurgical immunosuppressive microenvironment by inhibiting the activity of cyclo‐oxygenase‐2 (COX‐2). The aOX40 serves as an immune agonistic antibody and is released from the iSGel in a constant manner lasting for over 20 days. In a subcutaneous murine CRC model, the iSGels@aOX40 results in complete inhibition on tumor recurrence. In addition, the cured mice show resistance to tumor re‐challenge, suggesting that immune memory effects are established after the iSGels@aOX40 treatment. In an orthotopic CRC peritoneal metastatic model, the iSGels@aOX40 also remarkably inhibits the growth of the abdominal metastatic tumors, suggesting great potential for clinical CRC therapy.
Uranium In Situ Electrolytic Deposition with a Reusable Functional Graphene‐Foam Electrode
Authors: Wang Chao , Helal Ahmed S. , Wang Ziqiang , Zhou Jian , Yao Xiahui , Shi Zhe , Ren Yang , Lee Jinhyuk , Chang Jeng‐Kuei , Fugetsu Bunshi , Li Ju 
Published: August 2021

AbstractNuclear fission produces 400 GWe which represents 11% of the global electricity output. Uranium is the essential element as both fission fuel and radioactive waste. Therefore, the recovery of uranium is of great importance. Here, an in situ electrolytic deposition method to extract uranium from aqueous solution is reported. A functionalized reduced graphene oxide foam (3D‐FrGOF) is used as the working electrode, which acts as both a hydrogen evolution reaction catalyst and a uranium deposition substrate. The specific electrolytic deposition capacity for U(VI) ions with the 3D‐FrGOF is 4560 mg g−1 without reaching saturation, and the Coulombic efficiency can reach 54%. Moreover, reduction of the uranium concentration in spiked seawater from 3 ppm to 19.9 ppb is achieved, which is lower than the US Environmental Protection Agency uranium limits for drinking water (30 ppb). Furthermore, the collection electrode can be efficiently regenerated and recycled at least nine times without much efficiency fading, by ejecting into 2000 ppm concentrated uranium solution in a second bath with reverse voltage bias. All these findings open new opportunities in using free‐standing 3D‐FrGOF electrode as an advanced separation technique for water treatment.
Algorithms for activity correction models for geochemical speciation and reactive transport modeling
Authors: Carrayrou Jérôme , Bertagnolli Caroline , Fahs Marwan 
Published: August 2021

AbstractReactive transport softwares are today one of the cornerstones of environmental research. They contain multiphysics with very complex algorithms, including flow, transport, chemical, and sometimes heat transport, mechanical, and/or biological algorithms. Because of this complexity, some parts of these algorithms still have not been sufficiently studied. In this work, we focus on algorithms for activity correction, a specific subset of equilibrium chemistry algorithms. We show that the most used algorithm (the inner fixed‐point algorithm) or the most rigorous algorithm (the full Newton) might not be the most efficient, and we propose a new one, the outer fixed‐point algorithm, which is more robust and faster than other algorithms.
Technical note: Estimating original crown height in worn mandibular canines using aspects of dentin morphology
Authors: McFarlane Gina , Floyd Bruce , Smith Caitlin , Mahoney Patrick 
Published: August 2021

AbstractWe present a novel method to estimate original crown height (OCH) for worn human mandibular canines using a cubic regression equation based on ratios of worn crown height and exposed dentin. This method may help alleviate issues frequently presented by worn teeth in dental analyses, including those in bioarchaeology. Mandibular canines (n = 28) from modern day New Zealand and English populations were selected. Crown height and dentin thickness were measured on dental thin sections (n = 19) and the resulting (log10) ratios were fitted to a cubic regression curve allowing OCH in worn crowns to be predicted. Variation in the dentin apex position was recorded and effects of angled wear slopes investigated allowing adjusted values to be generated. Our method is trialed for use on intact and sectioned teeth (n = 17). A cubic regression curve best describes the relationship between (log10) ratios and crown height deciles (R2 = 0.996, df1 = 3, df2 = 336, p < 0.001). No significant differences were detected between OCH estimates using our method and digitally recreated cusp outlines of the same crowns (t = 1.024, df = 16, p > 0.05), with a mean absolute error of 0.171 mm and an adjusted coefficient of determination of 0.923. Our approach offers a quantitative method to estimate the percentage of OCH remaining on worn mandibular canines, and by extension, the OCH. Our estimates are comparable to digitally recreated cusps but less subjective and not limited to crowns with minimal wear.
Key Features of Polyimide‐Derived Carbon Molecular Sieves
Authors: Qiu Wulin , Leisen Johannes E. , Liu Zhongyun , Quan Wenying , Koros William J. 
Published: August 2021

AbstractCarbon molecular sieve (CMS) membranes have impressive separation properties; however, both chemical and morphology structures need to be understood better. Here we characterize CMS with the simplest polyimide (PI) PMDA/pPDA (PMDA=pyromellitic dianhydride, pPDA=p‐phenylenediamine), using FTIR, solid‐state 15N‐NMR and 13C‐NMR, XPS, XRD, and Raman spectra to study chemical structure. We also compare gas separation properties for this CMS to a CMS derived from a more conventional PI precursor. The detailed characterization shows the presence of aromatic pyridinic, pyrrolic rings as well as graphitic, pyridonic components and a few other groups in both CMS types derived from the very different precursors. The CMS morphologies, while related to precursor and pyrolysis temperature details, show similarities consistent with a physical picture comprising distributed molecular sieving plate‐like structures. These results assist in understanding diverse CMS membrane separation performance.
Tailoring the Local Environment of Platinum in Single‐Atom Pt1/CeO2 Catalysts for Robust Low‐Temperature CO Oxidation
Authors: Jiang Dong , Yao Yonggang , Li Tangyuan , Wan Gang , Pereira‐Hernández Xavier Isidro , Lu Yubing , Tian Jinshu , Khivantsev Konstantin , Engelhard Mark H. , Sun Chengjun , García‐Vargas Carlos E. , Hoffman Adam S. , Bare Simon R. , Datye Abhaya K. , Hu Liangbing , Wang Yong 
Published: August 2021

AbstractA single‐atom Pt1/CeO2 catalyst formed by atom trapping (AT, 800 °C in air) shows excellent thermal stability but is inactive for CO oxidation at low temperatures owing to over‐stabilization of Pt2+ in a highly symmetric square‐planar Pt1O4 coordination environment. Reductive activation to form Pt nanoparticles (NPs) results in enhanced activity; however, the NPs are easily oxidized, leading to drastic activity loss. Herein we show that tailoring the local environment of isolated Pt2+ by thermal‐shock (TS) synthesis leads to a highly active and thermally stable Pt1/CeO2 catalyst. Ultrafast shockwaves (>1200 °C) in an inert atmosphere induced surface reconstruction of CeO2 to generate Pt single atoms in an asymmetric Pt1O4 configuration. Owing to this unique coordination, Pt1δ+ in a partially reduced state dynamically evolves during CO oxidation, resulting in exceptional low‐temperature performance. CO oxidation reactivity on the Pt1/CeO2_TS catalyst was retained under oxidizing conditions.
A Congruent‐Melting Mid‐Infrared Nonlinear Optical Vanadate Exhibiting Strong Second‐Harmonic Generation
Authors: Wu Chao , Jiang Xingxing , Lin Lin , Hu Yilei , Wu Tianhui , Lin Zheshuai , Huang Zhipeng , Humphrey Mark G. , Zhang Chi 
Published: August 2021

AbstractStudy of mid‐infrared (mid‐IR) nonlinear optical (NLO) materials is hindered by the competing requirements of optimized second‐harmonic generation (SHG) coefficient dij and laser‐induced damage threshold (LIDT) as well as the harsh synthetic conditions. Herein, we report facile hydrothermal synthesis of a polar NLO vanadate Cs4V8O22 (CVO) featuring a quasi‐rigid honeycomb‐layered structure with [VO4] and [VO5] polyhedra aligned parallel. CVO possesses a wide IR‐transparent window, high LIDT, and congruent‐melting behavior. It has very strong phase‐matchable SHG intensities in metal vanadate family (12.0 × KDP @ 1064 nm and 2.2 × AGS @ 2100 nm). First‐principles calculations suggest that the exceptional SHG responses of CVO largely originate from virtual electronic transitions within [V4O11]∞ layer; the excellent optical transmittance of CVO arises from the special characteristics of vibrational phonons resulting from the layered structure.
Regio‐controllable Cobalt‐Catalyzed Sequential Hydrosilylation/Hydroboration of Arylacetylenes
Authors: Cheng Zhaoyang , Guo Jun , Sun Yufeng , Zheng Yushan , Zhou Zhehong , Lu Zhan 
Published: August 2021

AbstractRegiodivergent addition reactions provide straightforward and atom‐economic approaches to access different regioisomers. However, the regio‐chemistry control to access all the possible results is still challenging especially for the reaction involving multiple addition steps. Herein, we reported regio‐controllable cobalt‐catalyzed sequential hydrosilylation/hydroboration of arylacetylenes, delivering all the possible regio‐outcomes with high regioselectivities (up to >20/1 rr for all the cases). Each regioisomer of value‐added silylboronates could be efficiently and regioselectively obtained from the same materials. The adjustment of the ligands of cobalt catalysts combined with dual catalysis relay strategy is the key to achieve regio‐chemistry control. This regio‐controllable research might inspire the exploration of the diversity‐oriented synthesis that involves multiple additions and provide full sets of regioisomers of other synthetic useful molecules.
Feedback and Communication in Active Hydrogel Spheres with pH Fronts: Facile Approaches to Grow Soft Hydrogel Structures
Authors: Maity Indrajit , Sharma Charu , Lossada Francisco , Walther Andreas 
Published: August 2021

AbstractCompartmentalized reaction networks regulating signal processing, communication and pattern formation are central to living systems. Towards achieving life‐like materials, we compartmentalized urea‐urease and more complex urea‐urease/ester‐esterase pH‐feedback reaction networks into hydrogel spheres and investigate how fuel‐driven pH fronts can be sent out from these spheres and regulated by internal reaction networks. Membrane characteristics are installed by covering urease spheres with responsive hydrogel shells. We then encapsulate the two networks (urea‐urease and ester‐esterase) separately into different hydrogel spheres to devise communication, pattern formation and attraction. Moreover, these pH fronts and patterns can be used for self‐growing hydrogels, and for developing complex geometries from non‐injectable hydrogels without 3D printing tools. This study opens possibilities for compartmentalized feedback reactions and their use in next generation materials fabrication.
Quick and Easy Method to Dramatically Improve the Electrochemical CO2 Reduction Activity of an Iron Porphyrin Complex
Authors: Kosugi Kento , Kondo Mio , Masaoka Shigeyuki 
Published: August 2021

AbstractThe development of artificial molecular catalysts for CO2 reduction is the key to solving energy and environmental problems. Although chemical modifications can generally improve the catalytic activity of this class of compounds, they often require complicated synthetic procedures. Here, we report a simple procedure that dramatically enhances electrochemical CO2 reduction activity. A one‐step counteranion‐exchange reaction increased the solubility of a commercially available catalyst, iron(III) tetraphenylporphyrin chloride, in a variety of solvents, allowing the investigation of its catalytic performance under various conditions. Surprisingly, the turnover frequency for CO evolution in acetonitrile (MeCN) reached 7 300 000 s−1, which is the highest among those of current best‐in‐class molecular catalysts. This excellent catalytic activity originates from the unique reaction between the generated FeI species and CO2 in MeCN during catalysis. The present study offers a “quick and easy” method for obtaining an efficient catalytic system for electrochemical CO2 reduction.
Asymmetric Cation‐Olefin Monocyclization by Engineered Squalene–Hopene Cyclases
Authors: Eichenberger Michael , Hüppi Sean , Patsch David , Aeberli Natalie , Berweger Raphael , Dossenbach Sandro , Eichhorn Eric , Flachsmann Felix , Hortencio Lucas , Voirol Francis , Vollenweider Sabine , Bornscheuer Uwe T. , Buller Rebecca 
Published: August 2021

AbstractSqualene–hopene cyclases (SHCs) have great potential for the industrial synthesis of enantiopure cyclic terpenoids. A limitation of SHC catalysis has been the enzymes’ strict (S)‐enantioselectivity at the stereocenter formed after the first cyclization step. To gain enantio‐complementary access to valuable monocyclic terpenoids, an SHC‐wild‐type library including 18 novel homologs was set up. A previously not described SHC (AciSHC) was found to synthesize small amounts of monocyclic (R)‐γ‐dihydroionone from (E/Z)‐geranylacetone. Using enzyme and process optimization, the conversion to the desired product was increased to 79 %. Notably, analyzedAciSHC variants could finely differentiate between the geometric geranylacetone isomers: While the (Z)‐isomer yielded the desired monocyclic (R)‐γ‐dihydroionone (>99 %ee), the (E)‐isomer was converted to the (S,S)‐bicyclic ether (>95 %ee). Applying the knowledge gained from the observed stereodivergent and enantioselective transformations to an additional SHC‐substrate pair, access to the complementary (S)‐γ‐dihydroionone (>99.9 %ee) could be obtained.
Can One Measure Resonance Raman Optical Activity?
Authors: Li Guojie , Alshalalfeh Mutasem , Yang Yanqing , Cheeseman James R. , Bouř Petr , Xu Yunjie 
Published: August 2021

AbstractResonance Raman optical activity (RROA) is commonly measured as the difference in intensity of Raman scattered right and left circularly polarized light, IR−IL, when a randomly polarized light is in resonance with a chiral molecule. Strong and sometimes mono‐signate experimental RROA spectra of several chiral solutes were reported previously, although their signs and relative intensities could not be reproduced theoretically. By examining multiple light‐matter interaction events which can occur simultaneously under resonance, we show that a new form of chiral Raman spectroscopy, eCP‐Raman, a combination of electronic circular dichroism and circularly polarized Raman, prevails. By incorporating the finite‐lifetime approach for resonance, the experimental patterns of the model chiral solutes are captured theoretically by eCP‐Raman, without any RROA contribution. The results open opportunity for applications of eCP‐Raman spectroscopy and for extracting true RROA experimentally.
New 3‐(1H‐benzo[d]imidazol‐2‐yl)quinolin‐2(1H)‐one‐based triazole derivatives: Design, synthesis, and biological evaluation as antiproliferative and apoptosis‐inducing agents
Authors: Gaikwad Nikhil B. , Bansode Sapana , Biradar Shankar , Ban Mayuri , Srinivas Nanduri , Godugu Chandraiah , Yaddanapudi Venkata M. 
Published: August 2021

AbstractA series of 1,2,3‐triazole derivatives based on the quinoline–benzimidazole hybrid scaffold was designed, synthesized, and screened against a panel of NCI‐60 humanoid cancer cell lines for in vitro cytotoxicity evaluation, which revealed that compound Q6 was the most potent cytotoxic agent with excellent GI50, TGI, and LC50 values on multiple cancer cell lines. Q6 was tested further on the BT‐474 breast cancer line to evaluate the mechanism of action. Preliminary screening studies based on the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay revealed that compound Q6 had an excellent antiproliferative effect against human breast cancer cells, BT‐474, with IC50 values of 0.59 ± 0.01 μM. The detailed study based on the acridine orange/ethidium bromide staining (AO/EB) and the 4′,6‐diamidino‐2‐phenylindole (DAPI) assay suggested that the antiproliferative activity shown was due to the induction of apoptosis on exposure to Q6. Further, DCFDA staining showed the generation of reactive oxygen species, altering the mitochondrial potential and leading to the initiation of apoptosis. This was further supported by JC‐1 staining, indicating that this scaffold can contribute to the development of more potent derivatives.