bluuebunny/crossref_metadata_2025_split huggingface dataset:
HIGH-RESOLUTION SPECTROSCOPY OF THE B[e] STAR MWC 645
Authors: Nodyarov A.S. , Miroshnichenko A.S. , Khokhlov S.A. , Zharikov S.V. , Manset N. , Klochkova V.G. , Usenko I.A. 
Published: December 2021

Optical high-resolution spectroscopic observations of the emission-line star MWC645 are presented. The spectrum exhibits strong variable double-peaked Balmer emission lines as well as low-excitation emission lines of FeII, [FeII], and [OI] which are signatures of the B[e] phenomenon, while lines of helium have not been found. In addition to the emission lines, for the first time we identified absorption lines of neutral metals (e.g., LiI 6708 A, CaI 6717 A, and a number of FeI and TiI lines) that indicate the presence of a cool component in the system. The heliocentric radial velocity measured in our best spectrum was found to be −65.1±1.0 kms −1 for the emission lines and −23.2±0.4 kms −1 for the absorption lines. Using a combination of photometric and spectroscopic data as well as the Gaia EDR3 distance (D=6.5±0.9 kpc), we disentangled the component contributions and estimated their temperatures and luminosities (∼15000 K and ∼4000 K, log L/L ? = 3.8±0.2 and 2.8±0.2 for the hot and cool component, respectively).
The subcompartmented oxphosomic model of the phosphorylating system organization in mitochondria
Authors: Ukolova I. V. 
Published: December 2021

The oxidative phosphorylation (OXPHOS) system of mitochondria supports all the vitally important energyconsuming processes in eukaryotic cells, providing them with energy in the form of ATP. OXPHOS enzymes (complexes I–V) are located in the inner mitochondrial membrane, mainly in the cristae subcompartment. At present, there is a large body of data evidencing that the respiratory complexes I, III2 and IV under in vivo conditions can physically interact with each other in diverse stoichiometry, thereby forming supercomplexes. Despite active accumulation of knowledge about the structure of the main supercomplexes of the OXPHOS system, its physical and functional organization in vivo remains unclear. Contemporary models of the OXPHOS system’s organization in the inner membrane of mitochondria are contradictory and presume the existence of either highly organized respiratory strings, or, by contrast, a set of randomly dispersed respiratory supercomplexes and complexes. Furthermore, it is assumed that ATP-synthase (complex V) does not form associations with respiratory enzymes and operates autonomously. Our latest data obtained on mitochondria of etiolated shoots of pea evidence the possibility of physical association between the respiratory supercomplexes and dimeric ATP-synthase. These data have allowed us to reconsider the contemporary concept of the phosphorylation system organization and propose a new subcompartmented oxphosomic model. According to this model, a substantial number of the OXPHOS complexes form oxphosomes, which in a definite stoichiometry include complexes I–V and are located predominantly in the cristae subcompartment of mitochondria in the form of highly organized strings or patches. These suprastructures represent “mini-factories” for ATP production. It is assumed that such an organization (1) contributes to increasing the efficiency of the OXPHOS system operation, (2) involves new levels of activity regulation, and (3) may determine the inner membrane morphology to some extent. The review discusses the proposed model in detail. For a better understanding of the matter, the history of development of concepts concerning the OXPHOS organization with the emphasis on recent contemporary models is briefly considered. The principal experimental data accumulated over the past 40 years, which confirm the validity of the oxphosomic hypothesis, are also provided.
Effects of Potassium Fertilization, Cultivar Specifics and Seedling Temperature Regime on Growth Parameters and Yield of Tomato (Solanum lycopersicum L.)
Authors: Vasileva V. , Dinev N. , Mitova I. 
Published: December 2021

Background: Potassium fertilization shows beneficial effect on formation of tomato vegetative biomass and productivity. The purpose of this study was to determine the extent to which split potassium application and seedling temperature regime affects the growth parameters (leaf number, leaf area, fresh leaf weight and LAI) and yield of various tomato cultivars. Methods: A pot experiment was conducted to investigate the impact of single dose and split potassium fertilization treatments, cultivar specifics and seedling temperature regime on growth parameters and yield of tomato. Ten high yielding classic round shape tomato cultivars with determinate growth habitat were planted on Fluvisol. Single and split potassium fertilization treatments were tested. Result: The seedlings growth temperature regime and splitting the potassium fertilization treatment did not have a significant effect on the development of leaf biomass in tomato plants. Results revealed that leaf number, leaf area, fresh leaf weight and LAI of tomato plants was significantly affected by the cultivar genetic factor. Tomato yield were significantly affected by cultivars and split potassium fertilization treatments. Cultivars that measured the highest leaf area, fresh leaf weight and LAI and were also the highest yielding ones. A positive correlation between LAI and tomato fruit yield was observed.
Effect of Stress Shadow Caused by Multistage Fracturing from Multiple Well Pads on Fracture Initiation and Near-Wellbore Propagation from Infill Wells
Authors: Wang Xiaohua , Zhang Fengshou , Tang Meirong , Du Xianfei , Tang Jizhou 
Published: December 2021

Summary
Multistage fracturing with multiwell pads (MSFMP) is an essential technology for the efficient development of unconventional oil and gas reservoirs, but the reservoir area between two well pads is often not stimulated. Fracture initiation and near-wellbore propagation from infill horizontal wells drilled with different azimuth from the optimal azimuth in the unstimulated area is poorly understood, largely because of the stress shadow (or induced stress) caused by MSFMP. In this study, we propose an integrated method for calculating the stress shadow caused by MSFMP and then determine optimal completion parameters for infill horizontal wells in the unstimulated connecting area between two well pads. First, we develop a theoretical stress shadow model caused by MSFMP on the basis of the dislocation theory. Considering two extreme cases, fully open and completely closed propped fractures, the range of stress shadow in the unstimulated area after MSFMP of 20 horizontal wells in Platform H of tight reservoirs in the Changqing Oilfield, China, is considered as an example. Second, we import the calculated stress shadow into a 3D perforated fracturing model that is built based on the discrete lattice method. Then, we investigate the influence of perforation technology, horizontal wellbore azimuth, phase angle, and injection rate on fracture initiation and near-wellbore propagation. Our results show that this model is capable of calculating stress shadow at any position and then can be used to optimize the fracturing interval for the middle unstimulated area. We find that appropriate perforation and fracturing parameters significantly decrease the complexity of near-wellbore fractures. The models and results presented in this paper provide a new method and new insight for quantifying and optimizing fracture initiation and propagation for infill horizontal wells to maximize reservoir stimulation efficiency.
Penthorum chinense Pursh alleviates ethanol-induced hepatic oxidative impairment in zebrafish via AMPK / p62 / Nrf2 / mTOR pathway
Authors: zhao xingtao , zhou mengting , deng ying , guo chaocheng , liao li , he linfeng , peng cheng , li yunxi 
Published: December 2021

Abstract
Ethnopharmacological relevance: In China, Penthorum chinense Pursh (PCP) is renowned for its effectiveness in “promoting blood circulation” and “removing blood stasis”. It can “relieve the liver” and its application in the field of liver protection, including viral hepatitis, alcoholic liver, liver fibrosis, has been known for hundreds of years.Aim of the study: Oxidative stress is widely believed to exert a key role in the pathophysiology of alcoholic liver disease (ALD). Therefore, antioxidant therapy reflects a reasonable strategy for the prevention and treatment of ALD. Hence, this study aimed to elucidate the mechanism of PCP in ethanol-induced liver injury.Methods: Treatment of liver-specific transgenic zebrafish larvae (lfabp: EGFP) at three days post-fertilization (3 dpf) with different concentrations of PCP (100, 50, 25 μg / mL) for 48 h was followed by soaking in 350 mmol / L ethanol for 32 h. Liver function and fat accumulation were identified by phenotypic indicators and biochemical kits. The related proteins and gene expression were further estimated by western blotting and quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). Finally, high performance liquid chromatography (HPLC) was adopted to analyze the chemical composition of PCP extract.Results: Firstly, PCP mediated alleviation of ethanol-induced steatosis and reduction of aspartate aminotransferase (AST), alanine transaminase (ALT), total cholesterol (TC) and triglyceride (TG) related indexes were evident. Dose-dependent decrease of intracellular reactive oxygen species (ROS) production, the activity of malondialdehyde (MDA) and increased the activity of glutathione (GSH), Superoxide dismutase (SOD) and catalase (CAT) in zebrafish substantiated the role of PCP in relieving oxidative stress. Furthermore, PCP induced downregulation of sequestosome 1 (p62 / SQSTM1, p62), Atg13 and Beclin 1 expression promoted autophagy. Meanwhile, PCP contributed to the hepatoprotective function by downregulating the expression of kelch-like ECH-associated protein 1 (Keap1) and upregulating the expression of nucleus factor-E2-related factor 2 (Nrf2), which activated cytoprotective related gene HO-1. Moreover, HPLC of PCP extract confirmed the presence of various polyphenols with potential antioxidant effects. Finally, PCP appeared to promote the activated protein kinase (AMPK) / p62 / Nrf2 / mTOR signaling pathways, which were related to oxidative stress and autophagy in zebrafish.Conclusion: This study claimed that by activating the AMPK / p62 / Nrf2 / mTOR signaling pathway, PCP could attenuate ethanol-induced liver injury in zebrafish.
A Rough Variant of Nontoxigenic Vibrio Cholerae O139 Imported From Vietnam
Authors: Westerström Pontus , Dragsted Ulrik Bak 
Published: December 2021

Abstract
Purpose (stating the main purposes and research question)Vibrio cholerae is classified in O-antigen polysaccharide outer membrane properties where O1 and O139 are strains that cause pandemics and epidemics while non-O1/non-O139 usually cause mild disease. The dynamic evolution of Vibrio cholerae involves new virulence factors through horizontal gene transfer and formerly nontoxigenic serogroups are being reported as causing increasingly severe forms of human disease. Our purpose was to investigate serogroup and virulence factors in one imported isolate from Vietnam and compare these to virulence factors seen in different strains of Vibrio cholerae.MethodsWe have serotyped one isolate of imported Vibrio cholerae from Vietnam to Denmark and performed whole genome sequencing to identify known virulence genes. ResultsWe have identified virulence factors in our isolate that are recently discovered and one toxin, MakA, has not previously been reported from an Asian strain. The isolate was found to be a serogroup non-O1/non-O139 strain, however, sequence analysis gave a 96, 6% ID match of the wbfZ gene, stipulating our isolate to belong to either serogroup O22 or O139. ConclusionsLooking at the combination of virulence factors of the isolate we suggest the finding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version. This is a rare finding in a clinical isolate.
Proteomic Analysis of the Periodontal Pathogen Prevotella Intermedia Secretomes in Biofilm and Planktonic Lifestyles
Authors: Karched Maribasappa , Bhardwaj Radhika , Qudeimat Muawia , Al-Khabbaz Areej , Ellepola Arjuna 
Published: December 2021

Abstract
Prevotella intermedia is an important species associated with periodontitis. Despite the remarkable clinical significance, little is known about the molecular basis for its virulence. The aim of this study was to characterize the secretome of P. intermedia in biofilm and planktonic life mode. The biofilm secretome showed 109 proteins while the planktonic secretome showed 136 proteins. The biofilm and the planktonic secretomes contained 17 and 33 signal-peptide bearing proteins, 13 and 18 lipoproteins, respectively. Superoxide reductase, sensor histidine kinase, C40 family peptidase, elongation factor Tu, threonine synthase etc. were unique to biofilm. Of the ~30 proteins with predicted virulence potential from biofilm and planktonic secretomes, only 6 were common between the two groups, implying large differences between biofilm and planktonic modes of P. intermedia. From gene ontology biofilm secretome displayed a markedly higher percent proteins compared to planktonic secretome in terms of cellular amino acid metabolic process, nitrogen compound metabolic process etc. Inflammatory cytokine profile analysis revealed that only the biofilm secretome, not the planktonic one, induced important cytokines such as MIP-1a/MIP-1b, IL-1b, and IL-8. In conclusion, the revealed differences in the protein profiles of P. intermedia biofilm and planktonic secretomes may trigger further questions about molecular mechanisms how this species exerts its virulence potential in the oral cavity.
Kikuchi-Fujimoto Disease in the Eastern Mediterranean zone.
Authors: manasra Abdel rahman Al , Al-Domaidat Hamzeh , Ayadeh Asem , Qaoud Doaa Al , Shalakhti Majd Al , khatib Sohaib Al , Fataftah Jehad , Al-Taher Raed , Nofal Mohammad 
Published: December 2021

Abstract
Background
Kikuchi-Fujimoto disease (KFD) is a rare benign and self-limiting syndrome. We aim to review cases of KFD at our institution as a rare illness in the Arab ethnic descent, and to analyze reports from most countries in the east Mediterranean zone.
Methods
This is a retrospective study in which the histopathology database was searched for the diagnosis of KFD. A full review of KFD patients’ medical records was done. Data regarding demographic features, clinical presentation, laboratory findings, comorbidities and management protocols were obtained. Published KFD cases from east Mediterranean countries were discussed and compared to other parts of the world.
Results
Out of 1968 lymph node biopsies studied, 11 (0.6%) cases of KFD were identified. Mean age of patients with KFD was 32 years (4-59). 73% (8/11) were females. The disease was self-limiting in 5 patients (45%), corticosteroid therapy was needed in 4 patients (34%), one patient was treated with methotrexate and one with antibiotics. One patient died as a consequence of lymphoma.
Conclusion
KFD may still be under diagnosed in our part of the world, likely due to the non-specific mixed symptoms at presentation as well as to the lack of awareness among general practitioners and health care providers. Early diagnosis and treatment is essential to reduce associated morbidity.
Molecular Epidemiology of Hepatitis B Virus Among HIV Co-Infected and Mono-Infected Cohorts in Northwest Ethiopia
Authors: Belyhun Yeshambel , Liebert Uwe Gerd , Maier Melanie 
Published: December 2021

Abstract
Background
Hepatitis B virus (HBV) infection is a particular concern in human immunodeficiency virus (HIV) infected individuals. In Ethiopia, detailed clinical and virological descriptions of HBV prevailing during HIV co-infection and symptomatic liver disease patients are lacking. The aim of this study was to investigate HBV virological characteristics from Ethiopian HBV/HIV co-infected and HBV mono-infected individuals.
Methods
A total of 4105 sera from HIV positive individuals, liver disease patients, and blood donors were screened serologically for HBV. The overlapping polymerase/surface genome region of HBV from 180 infected individuals was extracted, amplified, and sequenced for genotypic analysis.
Results
The HBsAg seroprevalence was detected 43% in liver disease patients, 8.4% in blood donors, and 6.7% in HIV/HBV co-infected individuals. The occult HBV prevalence was 3.7% in HIV/HBV co-infected individuals and 2.8% in blood donors with an overall prevalence rate of 3.4%. A phylogenetic analysis showed three HBV genotypes; A (61.1%), D (38.3%) and E (0.6%). Genotype A belongs to subtypes A1 (99.1%) and A9 (0.9%), but genotype D showed heterogeneous subtypes; D2 (63.8%) followed by D4 (21.7%), D1 (8.7%), D3 (4.3%), and D10 (1.4%).
Conclusions
The HIV/HBV co-infected individuals and blood donors showed lower HBsAg seroprevalence compared to liver diseases patients. Occult HBV prevalence showed no difference between HIV/HBV co-infected and blood donor groups. This study demonstrated predominance distribution of HBV subtypes A1 and D2 in northwest Ethiopia. The observed virological characteristics could contribute for evidence-based management of viral hepatitis in Ethiopia where antiretroviral therapy guidelines do not cater for viral hepatitis screening during HIV co-infection.
Study on Metabolic Indexes of Obesity in Patients with Common Mental Disorders in Stable Stage
Authors: Li Xiaoling , shi Xiaojie , Tan Yukang , Yu Yang , Tang Chaohua , Xu Guohong , Zhang Xinglian , Liao Hairong , Mai Xiancong , Chen Wensheng , Luo Xin , Xu Caixia , Xie Guojun , Liang Jiaquan 
Published: December 2021

Abstract
Background The incidence rate of obesity has been increasing steadily year by year, and it is a serious worldwide public health problem，especially for people with mental disorders.Aim To explore the related factors of obesity by analyzing the metabolic indexes of patients with common mental disorders in stable stage. Methods 576 subjects with major depressive disorder (MDD), bipolar disorder (BD) or schizophrenia (SCZ) were included, who received fixed drug dose and routine drug treatment for 2 years or more. Their venous blood was collected, and the blood metabolic indexes were analyzed. Results BD and SCZ are more prone to obesity than MDD. Multiple linear regression analysis showed that the value of BMI increased with the increase of age(B = 0.084, p < 0.001), TG(B = 0.355, p = 0.024), LDL(B = 0.697, p < 0.001), LDH(B = 0.011, p = 0.002), SCr(B = 0.051, p < 0.001), UA(B = 0.014, p < 0.001), HbA1c(B = 0.702, p = 0.004) and hsCRP(B = 0.101, p < 0.001). And It decreased with the increase of HDL(B = -1.493, p < 0.001). Discussion People with mental disorders who take drugs are prone to obesity. They should regularly check blood indicators and strengthen weight management to reduce the risk of obesity and promote their health.
Factors Facilitating the use of Contraceptive Methods Among Urban Adolescents and Youth in Guinea: A Qualitative Study
Authors: Manet Hawa , Doucet Marie-Hélène , Bangoura Charlotte , Dioubaté Nafissatou , Ayadi Alison El , Sidibé Sidikiba , Millimouno Tamba Mina , Delamou Alexandre 
Published: December 2021

Abstract
BackgroundThe use of modern contraceptive methods among adolescents and youth is a public health priority to prevent unwanted pregnancies. To our knowledge, no study has ever explored and documented the promoting factors for contraceptive use among urban adolescents and youth in Guinea. The objective of this study was to explore the personal, community, and health system factors that promote the use of contraceptive methods among urban adolescents and youth in Guinea. MethodsWe conducted a qualitative exploratory and descriptive study with adolescents and youth aged 15 to 24 living in Conakry, Guinea. Data were collected through twenty-six individual in-depth interviews, and 10 group interviews with an additional eighty individuals, for a total of 106 participants. The investigation was conducted from June to October 2019. Both individual and group interviews were audio-recorded, and the verbatims were afterwards transcribed. Data was analyzed using the “thematic analysis” method (deductive and inductive approaches).ResultsThe personal factors favoring contraceptive use among adolescents and youth pertained to perceived benefits of the methods, knowledge of the family planning service channels, means to afford the cost of the method, and spouse/sexual partner approval. The community factors included peer suggestions about contraceptive methods, and socio-cultural beliefs about the method. The health system factors referred to access to free contraceptive methods, availability of methods, clinical competence and attitude of the health care provider to advise or administer methods, and proximity of family planning services to users’ place of residence. ConclusionsThis research shows that many adolescents and youth living in Conakry are sexually active and want to avoid unwanted pregnancy by using modern, traditional or cultural contraceptive methods. Access to free or affordable methods, discretion of method use, proximity and availability of methods, and suggestions of methods by peers are factors that motivate adolescents and youth to use contraception. This knowledge can inform policies and programs to improve the use of effective contraceptive methods by adolescents and youth living in Conakry, Guinea, and ultimately contribute to the optimal sexual and reproductive health of this population.
Treatment Strategies for Recurrent Ileocolic Intussusceptions and a Novel Operative Procedure
Authors: Demir Mesut , Akın Melih , Unal Aydin , Kaba Meltem Kalyoncu , Sever Nihat , Dokucu Ali İhsan 
Published: December 2021

Abstract
Background

Intussusception is the most common cause of intestinal obstruction between 6 months and 36 months of age. There is no defined etiology in at least 75-90% of patients. Recurrent intussusception occurs in 5-16% of all intussusceptions and the treatment strategy is controversial in this patient group. Treatment of continued recurrent intussusception is a challenging problem when no lead point is revealed despite recurrence. We aimed to review our 10 years of experience in recurrent intussusception and describe a new operative technique for recurrent intussusception cases without any lead points.
Results

We retrospectively reviewed the data of patients with recurrent intussusception in our referral pediatric surgery clinic between 2007 and 2017. Ultrasound-guided hydrostatic reduction was performed on all patients. Surgery was performed on those patients who had findings of acute abdomen and complete intestinal obstruction or 2 failed attempts of UGHR for diagnostic purposes if a pathologic lead point was suspected based on patient findings and age. Laparoscopy or laparotomy was performed according to surgeon preference and experience. A total of 87 UGHRs were performed. Thirty-three patients were admitted to our clinic due to recurrent intussusception. The mean age was 12.75±14.14 (6 -84) months, and 19 were males and 14 were females. Abdominal pain, agitation and vomiting were common symptoms. UGHR was performed on all 33 patients on at least 2 different occasions. The time between the first and second UGHR treatments was 42.6 ± 186.19 (0-899) days. The success rate of the second UGHR was 27 out of 33 patients (81.8%). Surgery was performed on 6 patients. Manual reduction was performed on 5 patients and one was operated by the laparoscopic reduction of intussusception and Meckel’s diverticulum resection.
Conclusions

Surgeons should try to find permanent solutions for patients with multiple recurrent intussusceptions that are resistant to treatment. Surgical excision of the lead point will help prevent recurrent intussusception. Satisfactory results can also be obtained by ultrasound-guided hydrostatic reduction even in patients with recurrences. Laparoscopy is helpful in diagnosis, detection of lead points and treatment of irreducible intussusception. This new operative technique can be satisfactory for recurrent intussusceptions without any lead points.