bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous stereo-EEG and high-density scalp EEG recordings to study the effects of intracerebral stimulation parameters
Authors: Parmigiani S. , Mikulan E. P. , Russo S. , Sarasso S. , Zauli F. M. , Rubino A. , Cattani A. , Fecchio M. , Giampiccolo D. , Lanzone J. , D’Orio P. , del Vecchio M. , Avanzini P. , Nobili L. , Sartori I. , Massimini M. , Pigorini A. 
Published: November 2021

AbstractBackgroundCortico-cortical evoked potentials (CCEPs) recorded by stereo-electroencephalography (SEEG) are a valuable clinical tool to investigate brain reactivity and effective connectivity. However, these invasive recordings are spatially sparse since they depend on clinical needs. This sparsity hampers systematic comparisons across-subjects, the detection of the whole-brain spatiotemporal properties of CCEPs, as well as their relationships with classic sensory evoked potentials.ObjectiveTo demonstrate that CCEPs recorded by high-density electroencephalography (hd-EEG) are sensitive to changes in stimulation parameters and compensate for the limitations typical of invasive recordings.MethodsSEEG and hd-EEG activities were simultaneously recorded during SPES in drug-resistant epileptic patients (N=36). Changes in stimulation parameters encompassed physical (pulse intensity and width), geometrical (angle and position with respect to white/grey matter) and topological (stimulated cortical area) properties. Differences were assessed by measuring the overall responses and the amplitude of N1 and N2 components of the CCEPs, and by their spectral profiles.ResultsWhile invasive and non-invasive CCEPs were generally correlated, differences in pulse duration, angle and stimulated cortical area were better captured by hd-EEG. Further, hd-EEG responses to SPES reproduced basic features of responses to transcranial magnetic stimulation and showed a much larger amplitude as compared to typical sensory evoked potentials.ConclusionsThe present results show that macroscale hd-EEG recordings are exquisitely sensitive to variations in SPES parameters, including local changes in physical and geometrical stimulus properties, while providing valuable information about whole-brain dynamics. Moreover, the common reference space across subjects represented by hd-EEG may facilitate the construction of a perturbational atlas of effective connectivity.HighlightsCCEPs recorded with hd-EEG and SEEG are correlated.hd-EEG recording is highly sensitive to changes in stimulation parameters.hd-EEG responses show higher amplitude responses with respect to non-invasive ones.Simultaneous recordings provide a fixed observation point across subjects.
A nuclear cAMP microdomain suppresses tumor growth by Hippo pathway inactivation
Authors: Drozdz Marek M. , Doane Ashley S. , Alkallas Rached , Desman Garrett , Bareja Rohan , Reilly Michael , Bang Jakyung , Yusupova Maftuna , You Jaewon , Wang Jenny Z. , Verma Akansha , Aguirre Kelsey , Kane Elsbeth , Watson Ian R. , Elemento Olivier , Piskounova Elena , Merghoub Taha , Zippin Jonathan H. 
Published: November 2021

SummarycAMP signaling pathways are critical for both oncogenesis and tumor suppression. cAMP signaling is localized to multiple spatially distinct microdomains, but the role of cAMP microdomains in cancer cell biology is poorly understood. We developed a tunable genetic system that allows us to activate cAMP signaling in specific microdomains. We uncovered a previously unappreciated nuclear cAMP microdomain that functionally activates a tumor suppressive pathway in a broad range of cancers by inhibiting YAP, a key effector protein of the Hippo pathway, inside the nucleus. We show that nuclear cAMP induces a LATS-dependent pathway leading to phosphorylation of nuclear YAP solely at serine 397, export of YAP from the nucleus, without YAP protein degradation. Thus, nuclear cAMP inhibition of nuclear YAP is distinct from other known mechanisms of Hippo regulation. Pharmacologic targeting of specific cAMP microdomains remains an untapped therapeutic approach for cancer, and since Hippo pathway deregulation can lead to oncogenesis and chemotherapeutic resistance, drugs directed at the nuclear cAMP microdomain may provide new avenues for the treatment of cancer.
Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock
Authors: Kaulich Eva , Ackley Brian D. , Tang Yi-Quan , Hardege Iris , Schafer William R. , Walker Denise S. 
Published: November 2021

AbstractBiological clocks are fundamental to an organism’s health, controlling periodicity of behavior and metabolism. Here, we identify two acid-sensing ion channels, with very different proton sensing properties, and describe their role in an ultradian clock, the defecation motor program (DMP) of the nematode Caenorhabditis elegans. An ACD-5-containing channel, on the apical membrane of the intestinal epithelium, is essential for maintenance of luminal acidity, and thus the rhythmic oscillations in lumen pH. In contrast, the second channel, composed of FLR-1, ACD-3 and/or DEL-5, located on the basolateral membrane, controls the intracellular Ca2+ wave and forms a core component of the master oscillator that controls timing and rhythmicity of the DMP. flr-1 and acd-3/del-5 mutants show severe developmental and metabolic defects. We thus directly link the proton-sensing properties of these channels to their physiological roles in pH regulation and Ca2+ signaling, the generation of an ultradian oscillator, and its metabolic consequences.One-Sentence SummaryTwo acid-sensing DEG/ENaC channels play distinct roles in controlling different aspects of rhythmic proton and Ca2+ oscillations in the C. elegans intestine.
Differential Methods for Assessing Sensitivity in Biological Models
Authors: Mester Rachel , Landeros Alfonso , Rackauckas Chris , Lange Kenneth 
Published: November 2021

AbstractDifferential sensitivity analysis is indispensable in fitting parameters, understanding uncertainty, and forecasting the results of both thought and lab experiments. Although there are many methods currently available for performing differential sensitivity analysis of biological models, it can be difficult to determine which method is best suited for a particular model. In this paper, we explain a variety of differential sensitivity methods and assess their value in some typical biological models. First, we explain the mathematical basis for three numerical methods: adjoint sensitivity analysis, complex-perturbation sensitivity analysis, and forward-mode sensitivity analysis. We then carry out four instructive case studies. (i) The CARRGO model for tumor-immune interaction highlights the additional information that differential sensitivity analysis provides beyond traditional naive sensitivity methods, (ii) the deterministic SIR model demonstrates the value of using second-order sensitivity in refining model predictions, (iii) the stochastic SIR model shows how differential sensitivity can be attacked in stochastic modeling, and (iv) a discrete birth-death-migration model illustrates how the complex-perturbation method of differential sensitivity can be generalized to a broader range of biological models. Finally, we compare the speed, accuracy, and ease of use of these methods. We find that forward-mode automatic differentiation has the quickest computation time, while the complex-perturbation method is the simplest to implement and the most generalizable.Author SummaryOver the past few decades, mathematical modeling has become an indispensable tool in the biologist’s toolbox. From deterministic to stochastic to statistical models, computational modeling is ubiquitous in almost every field of biology. Because model parameter estimates are often noisy or depend on poorly understood interactions, it is crucial to examine how both quantitative and qualitative predictions change as parameter estimates change, especially as the number of parameters increase. Sensitivity analysis is the process of understanding how a model’s behavior depends on parameter values. Sensitivity analysis simultaneously quantifies prediction certainty and clarifies the underlying biological mechanisms that drive computational models. While sensitivity analysis is universally recognized to be an important step in modeling, it is often unclear how to best leverage the available differential sensitivity methods. In this manuscript we explain and compare various differential sensitivity methods in the hope that best practices will be widely adopted. In particular, we stress the relative advantages of existing software and their limitations. We also present a new numerical technique for computing differential sensitivity.
Type I interferon shapes brain distribution and tropism of tick-borne flavivirus
Authors: Chotiwan Nunya , Rosendal Ebba , Willekens Stefanie M. A. , Schexnaydre Erin , Nilsson Emma , Lindqvist Richard , Hahn Max , Mihai Ionut Sebastian , Morini Federico , Zhang Jianguo , Ebel Gregory D. , Carlson Lars-Anders , Henriksson Johan , Ahlgren Ulf , Marcellino Daniel , Överby Anna K. 
Published: November 2021

AbstractViral tropism within the brain and the role(s) of vertebrate immune response to neurotropic flaviviruses infection is largely understudied. We combined multimodal imaging (cm-nm scale) with single nuclei RNA- sequencing to study Langat virus in wildtype and interferon alpha/beta receptor knockout (Ifnar−/−) mice to visualize viral pathogenesis and define molecular mechanisms. Whole brain viral infection was imaged by Optical Projection Tomography coregistered toex vivoMRI. Infection was limited to grey matter of sensory systems in wildtype mice, but extended into white matter, meninges and choroid plexus inIfnar−/−mice. Cells in wildtype displayed strong type I and II IFN responses, likely due toIfnbexpressing astrocytes, infiltration of macrophages andIfng-expressing CD8+NK cells, whereas inIfnar−/−, the absence of this response contributed to a shift in cellular tropism towards non-activated resident microglia. Multimodal imaging-transcriptomics exemplifies a powerful way to characterize mechanisms of viral pathogenesis and tropism.
A Predictive Energy Landscape Model of Metamorphic Protein Conformational Specificity
Authors: Wrabl James O. , Voortman-Sheetz Keila , Hilser Vincent J. 
Published: November 2021

Abstract“Metamorphic” proteins challenge state-of-the-art structure prediction methods reliant on amino acid similarity. Unfortunately, this obviates a more effective thermodynamic approach necessary to properly evaluate the impact of amino acid changes on the stability of two different folds. A vital capability of such a thermodynamic approach would be the quantification of the free energy differences between 1) the energy landscape minima of each native fold, and 2) each fold and the denatured state. Here we develop an energetic framework for conformational specificity, based on an ensemble description of protein thermodynamics. This energetic framework was able to successfully recapitulate the structures of high-identity enginerered sequences experimentally shown to adopt either Streptococcus protein GA or GB folds, demonstrating that this approach indeed reflected the energetic determinants of fold. Residue-level decomposition of the conformational specificity suggested several testable hypotheses, notably among them that fold-switching could be affected by local de-stabilization of the populated fold at positions sensitive to equilibrium perturbation. Since this ensemble-based compatibility framework is applicable to any structure and any sequence, it may be practically useful for the future targeted design, or large-scale proteomic detection, of novel metamorphic proteins.Impact StatementMetamorphic proteins are single amino acid sequences capable of adopting more than one structure at equilibrium. Detection and design of these molecules hold great promise for biological understanding and materials engineering, but to do so requires a thermodynamic framework capable of estimating the free energy differences between the two structures and the denatured state. We present such a framework, show it to be effective for the well-studied metamorphic protein GA/GB system, and suggest testable hypotheses for engineering novel fold-switch proteins.
Critical criteria for enhancing consumption intention in restaurants during COVID-19
Authors: Chou Sheng-Fang , Sam Liu Chih-Hsing , Lin Jun-You 
Published: November 2021

PurposeThe purpose of this study is to illustrate the different systems controlling coronavirus disease 2019 (COVID-19) and curbing the impact of the virus on the hospitality economy. The author’s clarified the critical attributes of the government, organization management system and consumer behaviour using mediation-moderation models and demonstrated how those critical attributes influenced customer consumption intention during COVID-19 in Taiwan.Design/methodology/approachDue to the impact of the COVID-19 pandemic, this research is mainly distributed through online questionnaires through Facebook and other social media channels to recruit volunteers. Second, the pre-test survey used 100 questionnaires collected from juniors and seniors from a university in northern Taiwan to make predictions. Third, this study also conducted a questionnaire validity analysis, which identified 9 criteria and 34 items. Fourth, the questionnaire collected samples for a total of three months. Structural equation modelling was used to test the hypotheses in a sample of 1,098 consumers in Taiwan.FindingsThis study considers government, enterprise and consumer levels and conducts relevant factor analysis from consumers’ perspectives to understand the changes in consumer behaviour under COVID-19 influence. Regarding mediation, this study finds that information and communication mediate the relationships between crisis management and COVID-19 impact. Regarding moderation, this study exposes the critical moderating part of human resources, that hygiene and safety strengthen the relationships between COVID-19 impact and attitude towards life and that perceived anxiety strengthens the relationship between attitude towards life and consumption intention.Practical implicationsDuring COVID-19, restaurants should cooperate with the government to reduce the risk of community infection. Therefore, the government also needs to cooperate with restaurant companies to enhance the industrial economy, actively communicate with consumers and provide correct and sufficient information. At the same time, restaurant enterprises also need to have sufficient human resource arrangements, hygiene and safety planning to eliminate consumers’ doubts.Originality/valueThese findings indicate that consumers’ consumption intention to eat out is affected by the COVID-19 impact and attitude towards life. This research also confirms that perceived anxiety has a mediating effect on the relationship between consumer attitudes towards life and consumption intentions. To improve the restaurant economic process, they should consider solutions to reduce consumers’ perception of the COVID-19 impact and fear of eating out.
Engineer-centered enterprise and context-based knowledge: the sources of Chinese competitiveness in grid equipment
Authors: Shen Qunhong , Jiang Ziying , Feng Kaidong 
Published: November 2021

Purpose
The purpose of this study is to explore the competitive source of Chinese firms in an industrial sector of complex product systems. It helps to reveal the organizational innovation developed by Chinese firms in coping with international competition and technological challenges.

Design/methodology/approach
The study uses a qualitative method of research. The evidences are mainly collected through interviews, field observation and document analysis.

Findings
A pattern of engineer-centered organization is the source of competitiveness of Nanrui (NR) Electric (NREC) in this study. The firm equips its front project teams, and now its overseas branches with developmental human resources and authorizes them the power of decision-making to leverage R&D projects. It is an emerging challenge to the traditional multi-national companies (MNC) pattern, and enables the Chinese firms to build their capabilities on context-based knowledge.

Research limitations/implications
As a single-case study paper, there are limitations about the external validity of its argument. Through the in-depth discussion of the NREC case, this paper aims to generate some clues for future study in the relevant academic community, which can be a useful step to formal theorizing and modeling. That is why the authors develop the paper on a single case. As future directions of research, comparative studies covering more cases not only within the power system control and protection industry but also among different complex technology products industrial sectors are really needed.

Practical implications
For innovative firms from developing countries like China, they need to develop institutional arrangements to incentivize engineers in the frontline, which may help them to build competence upon successful interaction with customers. During the era of globalization, such a pattern may generate special competitiveness over giant multi-nationals or global production networks (GPNs).

Originality/value
The research provides an instructive case on the Chinese rise in industrial sectors of complex product systems. Its findings can not only provide enlightenment for industrial catch-up in developing countries through organizational innovation but also help to initiate a reconsideration of the traditional theorizing of MNC and GPN.
Transient thermo-elasto-hydrodynamic analysis of a bidirectional thrust bearing in start-up and shutdown processes
Authors: Cao Jingwei , Zhai Liming , Luo Yongyao , Ahn Soo-Hwang , Wang Zhengwei , Liu Yan 
Published: November 2021

PurposeThe purpose of this paper is to reveal the transient thermo-elasto-hydrodynamic lubrication mechanism of a bidirectional thrust bearing in a pumped-storage unit, and to propose the transient simulation method of two-way fluid-solid-thermal interaction of thrust bearing.Design/methodology/approachThe transient fluid-solid-thermal interaction method is used to simulate the three-dimensional lubrication of the thrust bearing, during the start-up and shutdown process of a pumped storage unit. A pad including an oil hole is modelled to analyze the temporal variation of lubrication characteristics, such as the film pressure, thickness and temperature, during the transient operation process.FindingsThe injection of the high-pressure oil sufficiently affects the lubrication characteristics on film, in which the hysteresis phenomena were found between the start-up and shutdown possess.Originality/valueThis paper reveals the transient lubrication mechanism of tilting pad in a thrust bearing, by means of transient fluid-solid-thermal interaction method. Lubrication characteristics are simulated without assuming the temperature relationship between the oil film inlet and the outlet and the heat transfer on the pad free surface. This paper provides a theoretical basis for the safe design and stable operation of thrust bearings.
Development strategy for prefabricated construction projects: a tripartite evolutionary game based on prospect theory
Authors: Dou Yudan , Sun Xiliang , Ji Ankang , Wang Yuna , Xue Xiaolong 
Published: November 2021

PurposeOwing to multiple superiorities to traditional counterparts, prefabricated construction (PC) has gained increasing attention worldwide. The development of PC projects reflects the effects of both policy supervision and PC practice, which aids the government in reasonably identifying the key issues of PC's promotion and rationally improving the policy deployment. However, existing studies fail to address this aspect, especially lacking quantitative exploration. This study explores the micro mechanism of PC's promotion, from the perspective of developing PC projects.Design/methodology/approachA tripartite evolutionary game model based on prospect theory of the government, developers and contractors is constructed. After rigorous theoretical deduction, this study adopts Changchun in China as a case city and collects the data using the Delphi technique, policy documents and literature analysis.FindingsResults indicate that contractors are generally willing to implement PC projects and the government chooses to actively supervise PC's promotion. The negative investment behavior of developers is the main obstacle to promote PC in Changchun currently.Practical implicationsThe conclusions are applicable to other comparable regions. This study is of value to promote PC with high efficiency and effect.Originality/valueThe tripartite evolutionary game model based on prospect theory proposed in this study is conducive to reveal the essence of PC's promotion. This is an important breakthrough in extant studies, with a broad applicability in the PC domain beyond China.
Optimisation of porous slab parameters for jet-impingement microchannel heat sink performance enhancement
Authors: Pandey Jyoti , Ansari Mohd. Zahid , Husain Afzal 
Published: November 2021

Purpose
Porous media can provide excellent performance in thermal energy transport applications. This study aims to optimise the square porous slabs (placed in the middle of the channel) parameters to enhance the cooling performance of the jet-impingement microchannel heat sink.

Design/methodology/approach
Three levels of each design parameters, i.e. porous slab side, porous slab height, type of material, permeability and quadratic drag factor, are studied; and an L27 orthogonal array is adopted to generate the design points in the specified design space. Optimum designs of the porous media slabs are achieved to minimise the maximum-wall temperature, thermal resistance and pressure drop and maximise the average heat transfer coefficient and figure of merit (FOM).

Findings
Results exhibited that the porous media material and permeability are the most, whereas drag factor is the least significant factors with respect to the overall performance of the heat sink. The optimum value of FOM for the proposed hybrid heat sink model belongs to the set of design variables, i.e. 0.4 mm slab side, 0.6 mm slab height, 5 × 10−11 m2 permeability, 0.21 drag factor and copper as substrate material.

Originality/value
This study proposes a novel design and a hybrid approach to investigate and optimise the hydrothermal performance of jet impingements on porous slabs inserted in the microchannels.
The learning organization and organizational learning in the public sector: a review and research agenda
Authors: Anand Amitabh , Brix Jacob 
Published: November 2021

PurposeThis study aims to propose a new research agenda for the theories of “organizational learning” and the “learning organization” in relation to the public sector. The research agenda can be used by researchers to make explicit accounts of how and where the results from their studies advance the current state-of-the-art in the intersection between public sector and organizational learning and/or the learning organization.Design/methodology/approachA combined systematic and bibliometric review methodology is applied based on the research that has been published in the past three decades.FindingsThrough an analysis of 238 journal publications obtained from the Scopus database, the authors determine the leading authors, countries, highly cited papers and take the stock of current literature. Similarly, by analyzing papers published between 2010 and 2020, the authors identify current tendencies and emerging themes of organizational learning and learning organization in the public sector and offer avenues for future research.Originality/valueTo the best of the authors’ knowledge, this is potentially the first, fully refereed study published reporting on a bibliometric and a systematic review of organizational learning and learning organization in the public sector.