bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coal Worker's Pneumoconiosis Mimicking Lung Cancer in a 75‐Year‐Old Woman With a History of Breast Cancer: A Clinical Case Report
Authors: Pakbaz Yeganeh , Moodi Farzan 
Published: January 2025

ABSTRACTPneumoconiosis, caused by inhaling mineral dust, remains a significant occupational disease, despite a declining incidence. Coal workers' pneumoconiosis (CWP), a common subtype, varies in presentation from simple to complicated forms. Differential diagnosis is crucial, especially when CWP manifests as lung masses mimicking malignancy. We present a case of CWP in a 75‐year‐old female with a history of breast cancer, initially suspected of lung cancer due to an incidental mass on chest radiography. Clinical examination, laboratory tests, chest tomography, and biopsy were conducted. The patient, with a history of biomass fuel exposure, presented with a left upper lung mass, initially thought to be lung cancer. Biopsy‐induced hemopneumothorax led to intensive care admission. Histopathology confirmed CWP and conservative management resulted in recovery. Imaging revealed a solid mass in the left upper lung with lymphadenopathy. Histopathology showed carbon‐laden macrophages and anthracosis, consistent with CWP. Imaging modalities, including MRI and FDG‐PET/CT, aid in differentiating CWP from cancer. CWP, mimicking lung cancer, underscores the importance of accurate diagnosis. Imaging features, including nodules with calcifications, guide diagnosis. MRI and FDG‐PET/CT offer valuable insights, albeit with limitations, emphasizing the need for judicious use based on clinical suspicion.
Herpetic Meningoencephalitis Complicating the Resection of a Vestibular Schwannoma: A Case Report
Authors: Houdu Jérôme , Barron Maxime , Civit Thierry , Parietti‐Winkler Cécile 
Published: January 2025

ABSTRACTAfter surgery involving cranial nerves and more generally the central nervous system, nonbacterial meningitis should raise suspicion of herpes simplex virus type 1 reactivation. No time should be wasted in diagnosis and treatment; therefore, a polymerase chain reaction testing on cerebrospinal fluid should be systematic in this situation, without neglecting to consider other differential diagnoses.
Extracranial Thrombosed Vertebral Aneurysm Associated Neurofibromatosis Type1 Treated by Neuroendovascular Coil Embolization: A Case Report and Review of Literature
Authors: Sano Kenshi , Kuge Atsushi , Kondo Rei , Yamaki Tetsu , Nakamura Kazuki , Saito Shinjiro , Sonoda Yukihiko 
Published: January 2025

ABSTRACTA 46‐year‐old NF1 patient with sudden visual disturbance had a thrombotic vertebral artery aneurysm causing cerebral infarction. Endovascular internal trapping was performed successfully, with complete recovery and no neurological deficits. Although this is a rare case, it suggests that endovascular therapy could be successful for vascular lesions complicated by NF1.
Undetermined Ruptured Low‐Grade Appendiceal Mucinous Neoplasm Following High‐Energy Blunt Abdominal Trauma Requiring Emergency Laparotomy
Authors: Matsuo Ippei , Yumoto Tetsuya , Tsuji Akari , Tanabe Ryo , Matsumura Toshihisa , Shimabara Mikoto , Akai Masaaki , Takagi Shoji , Naito Hiromichi , Nakao Atsunori 
Published: January 2025

ABSTRACTBlunt abdominal trauma causing intraperitoneal injury and/or bleeding can be life‐threatening, requiring immediate intervention. Diagnosing these cases can be challenging, especially when pre‐existing conditions are involved. Low‐grade appendiceal mucinous neoplasm (LAMN) is a rare tumor of the appendix that can lead to pseudomyxoma peritonei. Herein, we present a case of ruptured LAMN following blunt abdominal trauma after a high‐energy head‐on collision, complicating the differentiation from other intraperitoneal injuries. A 42‐year‐old Japanese female was brought to our hospital following high‐energy head‐on collision. She presented with stable vital signs, complaining of anterior chest pain and abdominal tenderness without peritoneal irritation. Computed tomography scans indicated multiple fractures in her chest and complex fluid around the Douglas fossa extending to the ileocecal area, with a slightly dilated appendix tip. Despite stable vitals, emergency laparotomy was needed for suspected peritonitis and/or intraperitoneal hemorrhage. Emergency laparotomy revealed yellowish, jelly‐like ascites and a ruptured appendiceal tumor. LAMN was suspected, and the appendix was completely resected, with cytoreductive surgery carefully performed. Histopathological examination confirmed the diagnosis of LAMN. Postoperative course was uneventful, and the patient was discharged on Day 13 and referred for further LAMN management. This case report highlights the diagnostic difficulties of LAMN rupture following blunt abdominal trauma, stressing the need to consider rare conditions like LAMN in differential diagnoses of acute abdomen posttrauma.
Learnings From the Co‐Development of Priority Risks in Australia's First National Climate Risk Assessment
Authors: Lin Brenda B. , Fleming Aysha , Romanach Lygia , Boulaire Fanny A. , Capon Tim , Po Murni , Cook Stephen , Darbyshire Rebecca , Bluhm Sonia , Barnett Guy 
Published: January 2025

ABSTRACTAustralia's first National Climate Risk Assessment is built on the latest science as well as learnings from other countries’ national risk assessments. The goal of the risk assessment was to identify the priority risks of climate change to Australia as a nation. Due to timeline obligations, this process needed to be completed in 4 months, a considerably shorter timeframe than other national climate risk assessments. In this paper, the authors share learnings from the process of implementing the first pass of Australia's National Climate Risk Assessment, which brought together more than 240 stakeholders across eight systems to co‐develop a set of national priority risks. These learnings are used to provide recommendations and advice for working at the national scale and within short timeframes. First, a rapid climate risk assessment can bring together a significant diversity and range of stakeholders to engage in a national process and provide a broad perspective of the priorities that should be pursued. Second, the design of the process can provide multiple opportunities to iterate through drafts of risks in rapid succession. Third, bringing stakeholders into discussion across systems can increase understanding of how risks are connected and how future work could be pursued across systems for more effective risk management and adaptation planning. Our learnings help inform how future climate risk assessments can embrace the complexity of systemic risks and highlight the importance of building stakeholder networks to support both the risk assessment process and the adaptation work that follows.
Treatment patterns and outcomes in secondary acute myeloid leukemia arising after hypomethylating agents: PETHEMA registry study
Authors: Lloret‐Madrid Pilar , Boluda Blanca , Martínez‐López Joaquín , Bergua Juan , Rodriguez Arboli Eduardo , Labrador Jorge , Sossa Claudia , Gil Cristina , Algarra Lorenzo , Lavilla‐Rubira Esperanza , Serrano Josefina , de Rueda Beatriz , Ibañez Francisco , González González Bernardo J. , Amigo María Luz , García Belmonte Daniel , Rodríguez‐Medina Carlos , Gómez‐Roncero María I. , Colorado Mercedes , López Lorenzo José L. , Tormo Mar , Arce Fernández Olga , Cano‐Ferri Isabel , Solana‐Altabella Antonio , Foncillas Maria‐Angeles , Alfonso Pierola Ana , Vives Susana , Núñez Marta Valero , López‐Vidal Hernán , Pérez‐Santaolalla Esther , Hermosín Ramos Lourdes , García Boyero Raimundo , Llorente González Laura , Roldán Alicia , Calderón Soledad Casado , Infante Joana Brioso , Olave Teresa , García‐Suárez Julio , de Laiglesia Almudena , Rodríguez‐Veiga Rebeca , Trigo Fernanda , Martínez‐Cuadrón David , Montesinos Pau 
Published: January 2025

AbstractBackgroundPatients with secondary acute myeloid leukemia who previously received hypomethylating agents for prior myeloid neoplasms (HMA‐sAML) face a dismal prognosis.MethodsThe authors analyze the characteristics, therapeutic approaches, and outcomes of patients with HMA‐sAML from the Programa Español para el Tratamiento de Hemopatías Malignas (PETHEMA) registry.ResultsA total of 479 patients were included, mostly from prior myelodysplastic syndrome (84%). Frontline therapy consisted of intensive chemotherapy (IC) in 31%, low‐dose cytarabine‐based in 19%, supportive care and clinical trial 17% each, and HMA‐based therapy in 12% and 4% in venetoclax‐based regimen. Complete remission was achieved in 95 patients (27%), with higher rate among IC and venetoclax‐based groups (44% and 41%, respectively). The median overall survival (OS) was 4.93 months, with 7.68 months for IC patients, 7.82 months after HMA monotherapy, and 4.66 months after venetoclax‐based regimens. Patients who underwent allogeneic hematopoietic stem cell transplantation in first remission (n = 33, 9%) had a better survival outcome (median OS not reached). Multivariate analyses identified age (≥65 years), Eastern Cooperative Oncology Group >2, higher white blood cell count, and adverse risk cytogenetic as adverse prognostic factors, whereas NPM1 mutation was a favorable factor.ConclusionsPatients with HMA‐sAML have a poor prognosis and suboptimal outcomes with conventional treatments, including BCL2 inhibitors, highlighting the need for clinical trials targeting this population.
Understanding the experience of Latinas in medical education: A qualitative study
Authors: Geiger Gabriella , Revette Anna , Nava‐Coulter Brett , Kiel Lauren , Kaufman Rebekah , Morabito Angela , Horiguchi Miki , Martinez‐Aceves Celia , Meza Kelly , Fakorede Olayinka , Christophers Briana , Orellana Priscilla , Pinzon Maria Mora , Lubner Sam J. , Florez Narjust 
Published: January 2025

AbstractBackgroundDespite increased recruitment of Latina medical students, the percentage of Latina physicians has remained stagnant, suggesting unique retentive barriers affecting this population. Discriminatory experiences involving bias may contribute to difficulties in the retention and advancement of Latinas in medicine. This qualitative analysis aimed to explore thematic barriers prevalent among Latinas throughout their medical training in the United States.MethodsAn anonymous online survey was distributed to Latinas in the continental United States from June 22 to August 12, 2022. Eligibility criteria included self‐identifying as Hispanic/Latina, female/woman, and being currently enrolled in or graduated from medical school, residency, or fellowship in the United States in the past 10 years. Content and thematic analyses were done on 602 responses across seven open‐ended survey questions.ResultsData were categorized into three main themes: (1) barriers stemming from limited social, cultural, and financial capital; (2) experiences of discrimination, bias, and perceptions of being undervalued; and (3) burdens resulting in notable mental health challenges such as depression and anxiety. Many women questioned their place in medicine throughout training and the value of the sacrifice and trauma endured.ConclusionThis is the first qualitative study evaluating the experiences of Latinas throughout medical training, revealing that they experience disproportionate barriers and discriminatory experiences from both colleagues and patients. Despite calls to enhance institutional climates of diversity, there continues to be an acute need for the reform of medical education to promote an inclusive culture and provide adequate mentorship to marginalized trainees.
A prospective study on diagnostic accuracy of technology‐enabled early detection of oral cancer and epidemiology of tobacco and other substances use in rural India
Authors: Khanna Divya , Mishra Aseem , Birur Praveen , Shruti Tulika , Gurushanth Keerthi , Mukhia Nirza , Pathak Ruchi , Gurmeet Singh Arjun , Shetty Anupama , Pradhan Satyajit , Chaturvedi Pankaj 
Published: January 2025

AbstractBackgroundLip and oral cavity cancer is leading cause of cancer mortality among Indian men. This study evaluated diagnostic accuracy of mobile health (mHealth) enabled screening for early detection of oral premalignant lesions or oral cancer (OPML/OC). It also described epidemiology of tobacco and other substance use and associated oral lesions in rural northern India.MethodsA prospective study enrolled 10,101 high‐risk individuals from rural settings of Varanasi district, India, between February 2021 and June 2023. Trained field workers captured habits information and oral cavity images and provided screening as suspicious or nonsuspicious on mHealth. Onsite experts and remote specialists provided clinical diagnoses. Diagnostic accuracy of mHealth‐enabled screening was evaluated. A subset of 252 participants was followed to assess changes in oral lesions.ResultsPrevalence of substance use was 55.7%, with 21.4% having OPML/OC. Sensitivity of field workers and remote diagnosis for detecting OPML/OC was moderate when compared with onsite expert. Overall, interobserver agreement was substantial. During follow‐up, the remote specialists identified 30 new and 13 progressive lesions with a significant decline in the red mean parameter of red, green, and blue colour ratios.ConclusionAlthough mHealth‐enabled screening demonstrated lower sensitivity in detecting OPML/OC, their high specificity and expanded access to screening positions mHealth as a valuable tool for improving oral cancer screening coverage in Varanasi. This is particularly crucial given the high burden of oral cancer driven by prevalent smokeless tobacco and areca nut use and the current lack of effective population‐based screening programs in this region.
Feasibility of a cancer screening program using multicancer early detection testing and whole‐body magnetic resonance imaging in a high‐risk population
Authors: Raz Dan J. , Nehoray Bita , Ceniceros Aaron , Motarjem Pejman , Landau Shana , Nelson Rebecca A. , Gray Stacy W. 
Published: January 2025

AbstractBackgroundThe authors assessed the feasibility, acceptability, and impact on cancer worry of a cancer screening program using multicancer early detection (MCED) tests and whole‐body magnetic resonance imaging (WBM) in individuals at high cancer risk because of family history or germline variants in cancer‐susceptibility genes.MethodsThis prospective trial enrolled participants aged 50 years and older who had a significant family history of cancer or a cancer‐susceptibility gene variant. Participants underwent noncontrast WBM and MCED testing. The results were shared with participants, and further imaging or consultations were conducted as needed. Surveys assessing anxiety, cancer worry, and acceptability of the intervention were completed at baseline and 6 months after testing.ResultsOne hundred participants were enrolled: 98 completed both WBM and MCED testing, and 89 completed their 6‐month follow‐up. The median age of participants was 62 years (range, 51–83 years), and 64% were women. Four participants (4%) were diagnosed with cancer based on WBM findings and subsequent work‐up, and all four underwent surgical resection. Two intraductal papillary mucinous neoplasms of the pancreas were detected and are being monitored. MCED testing was positive in four participants, none of whom had suspicious findings on magnetic resonance imaging. One participant with a JAK2 mutation and thrombocytosis is under monitoring for potential hematologic malignancy. Sixty‐two participants (85%) somewhat/strongly agreed that study participation reduced cancer worry. Composite Cancer Worry Scale scores demonstrated decreased worry at 6 months compared with baseline (51% vs. high worry in 69%; p < .001).ConclusionsMCED and WBM testing were feasible, acceptable, and were associated with decreased cancer worry at 6 months (clinical trials registration: NCT05868486).
Same‐day molecular testing for targetable mutations in solid tumor cytopathology—The next frontier of the rapid on‐site evaluation
Authors: Iorgulescu J. Bryan , Yang Richard Kenneth , Roy‐Chowdhuri Sinchita , Sura Gloria Hopkins 
Published: January 2025

AbstractIntroductionThis study aimed to assess the feasibility of implementing the Idylla system, an ultra‐rapid, cartridge‐based assay, as an extension of rapid on‐site evaluation (ROSE) in cytology. The authors conducted a pilot validation study on specimens from non–small cell lung carcinoma, thyroid carcinoma, and melanoma, evaluating four assays designed to detect alterations in KRAS, EGFR, BRAF, gene fusions, and expression imbalances in ALK, ROS1, RET, NTRK1/2/3, and MET exon 14 skipping transcripts. They investigated the feasibility of providing accurate biomarker molecular testing results in a cytopathology laboratory within hours of specimen collection.MethodsThe authors evaluated the performance characteristics and turn‐around‐time of the Idylla system by testing a total of 144 cartridge assays across various specimen types, including fine‐needle aspirate smears, formalin‐fixed paraffin‐embedded (FFPE) cell blocks, small tissue biopsy FFPE blocks, and control cell line FFPE scrolls.ResultsThe average time from specimen input to results output was 2–3 hours. Accuracy across the four cartridge types was: KRAS assay: 100%, EGFR assay: 94%, BRAF assay: 100%, and GeneFusion assay: 94%. Analytical sensitivity ranged from 1% to 5% variant allele frequency for all assays. Inter‐assay precision and analytical specificity were both 100%.ConclusionUsing the Idylla system, actionable genetic alterations can be reliably detected within 2–3 hours from cytology and small biopsy samples with minimal input requirements. The findings of this study demonstrate the feasibility of incorporating same‐day molecular testing as part of ROSE procedures in the cytopathology laboratory, ultimately shortening the time from procedure to personalized treatment for cancer patients.
A High‐Performance Electrochemical Sensor Based on Ni−Pt Bimetallic Nanoparticles Doped Metal Organic Framework ZIF‐8 for the Detection of Dopamine
Authors: Wannassi Jassem , Missaoui Nadhem , Mabrouk Chama , Castilla‐Martinez Carlos A. , Moumen Youssra , Echouchene Fraj , Barhoumi Houcine , Demirci Umit B. , Kahri Hamza 
Published: January 2025

AbstractIn this paper, microporous Zn‐based zeolitic imidazolate framework with the sodalite cage structure (SOD‐ZIF‐8) was synthesized by the solvothermal method. Powder X‐ray diffraction (PXRD), scanning electron microscopy (SEM) and N2 adsorption were employed to characterize the synthesized material. An ultra‐sensitive electrochemical sensor based on highly dispersed bimetallic Ni−Pt nanoparticles immobilized on zeolitic metal–organic framework ZIF‐8 for dopamine quantification is introduced for the first time. The as‐prepared Ni−Pt@ZIF‐8 composite was deposited onto a glassy carbon electrode (GCE), serving as a sensor that exhibits superior properties for the detection of dopamine (DA). A Box‐Behnken design was employed, and response surface methodology (RSM) was applied to investigate the impact of various experimental parameters on dopamine detection. The parameters optimized in this study included pH, drying time (hours), drop volume for deposition (μL), and accumulation time (minutes). The Box‐Behnken experimental design enabled the systematic optimization of these factors to enhance the sensor‘s performance. Benefiting from the synergy of ZIF‐8 and Ni−Pt bimetallic nanoparticles, the Ni−Pt@ZIF‐8 composite exhibited high sensitivity towards dopamine, achieving a low detection limit of 1.0 nM. The sensor‘s linear response to dopamine (1 nM to 10 μM), resistance to interference, and high recovery in human serum, coupled with its simple fabrication, make it a promising tool for real‐world dopamine detection.
Simple Isolation of Human Bone Marrow Adipose Tissue–Derived Mesenchymal Stem/Stromal Cells
Authors: Tonyalı Gülsena , Kiliç Emine , Muratoğlu Bihter , Alpdündar‐Bulut Esin , Özdemir Cansu , Uçkan‐Çetinkaya Duygu 
Published: January 2025

AbstractBone marrow adipose tissue (BMAT) has garnered significant attention due to its critical roles in leukemia pathogenesis, cancer metastasis, and bone marrow failure. BMAT is a metabolically active, distinct tissue that differs from other fat depots. Marrow adipocytes, closely interacting with hematopoietic stem/progenitor cells and osteoblasts, play a pivotal role in regulating their functions. However, standardized methods for isolating and defining human BMAT (hBMAT) remain unclear. In animal models, BMAT is commonly isolated directly from the bone marrow through flushing, enzymatic digestion, or mechanical disruption. In humans, BMAT isolation often involves the adipogenic induction of bone marrow mesenchymal stem/stromal cells (BM‐MSCs) derived from bone marrow aspirates. However, this approach reflects cellular responses to chemical stimuli and may not accurately represent in vivo differentiation potential. Similarly, BMAT obtained from hip or knee replacement surgeries might not reflect basal physiological conditions due to inflammatory influences. Here, we describe a direct method for culturing BMAT from the fatty layer of bone marrow aspirates obtained from healthy transplant donors. This protocol employs centrifugation and washing steps using basic laboratory equipment, offering simple and non‐enzymatic approach. For validation, isolated cells are characterized according to the International Society for Cell & Gene Therapy (ISCT) criteria. © 2025 Wiley Periodicals LLC.Basic Protocol 1: Isolation of human BMAT‐MSCs from the fatty layer of the bone marrowBasic Protocol 2: Culture expansion, trypsinization, and cryopreservation of BMAT‐MSCsSupport Protocol 1: Immunophenoypic characterization of human BMAT‐MSCs by flow cytometrySupport Protocol 2: In vitro characterization of multilineage differentiation potential of human BMAT‐MSCsSupport Protocol 3: Further characterization of gene expression in human BMAT‐MSCs using qRT‐PCR
Effectiveness of a novel traction device (TRACMOTION) for endoscopic submucosal dissection using a scissor‐type knife: An animal pilot study and clinical experiences
Authors: Miura Yuko , Tsuji Yosuke , Miyata Ryohei , Fujisawa Ayano , Tsukihara Hiroyuki , Fujishiro Mitsuhiro 
Published: January 2025

AbstractA newly developed articulated through‐the‐scope traction device, TRACMOTION, has been used clinically for endoscopic submucosal dissection (ESD). However, there are few reports on the characteristics of this device and the lesion types for which it is most effective. Therefore, we evaluated its optimal use, efficacy, and safety clinically in animals. Subsequently, we confirmed the safety and efficacy of ESD using this device in humans. Two live swine were used; one underwent conventional ESD (C‐ESD) and the other traction‐assisted ESD (T‐ESD). To examine the traction effect at each resection site, three ESD ulcers (greater curvature/anterior wall/posterior wall) with a diameter of approximately 40 mm were created in each swine. Based on our preliminary experiments, scissor‐type and needle‐type knives were used in the T‐ESD and C‐ESD groups, respectively. The primary endpoint was the resection speed, and the secondary endpoint was the degree of muscle layer damage. T‐ESD was faster than C‐ESD on the posterior wall, similar to the greater curvature, and T‐ESD was slower than C‐ESD on the anterior wall. There were no cases of intraoperative perforations. Obvious muscle layer damage was observed in post‐C‐ESD wounds on the anterior wall. The submucosal layer was retained in all post‐T‐ESD wounds. Although this study showed that ESD with TRACMOTION may be safe and effective, it is necessary to consider that it may be unsuitable for some lesions. This may be due to device characteristics and requires further validation.