bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trophoblast Cell-surface Antigen 2 Expression in Advanced Lung Cancer Patients and the Effects of Anti-cancer Treatments
Authors: Omori Shota , Muramatsu Koji , Kawata Takuya , Miyawaki Eriko , Miyawaki Taichi , Mamesaya Nobuaki , Kawamura Takahisa , Kobayashi Haruki , Nakashima Kazuhisa , Wakuda Kazushige , Ono Akira , Kenmotsu Hirotsugu , Naito Tateaki , Murakami Haruyasu , Sugino Takashi , Takahashi Toshiaki 
Published: March 2021

Abstract
Background: Trophoblast cell-surface antigen 2 (TROP2) is expressed on the surface of trophoblast cells and many malignant tumor cells. However, data on TROP2 expression in advanced lung cancer is insufficient, and its changes have not been fully evaluated. Methods: We assessed the prevalence and changes in TROP2 expression in lung cancer patients receiving anti-cancer treatments using immunohistochemical (IHC) analysis with an anti-TROP2 (clone: SP295). IHC scores were graded from 0–3; grade ≥2 was considered positive for TROP2 expression. We defined a difference in IHC score, before and after anti-cancer treatments, as the change in TROP2 expression.Results: Before anti-cancer treatment, TROP2 expression was observed in 89% (143/160) of patients and was significantly more common in adenocarcinoma and squamous cell carcinoma than in neuroendocrine carcinoma (P < 0.001). After anti-cancer treatment, TROP2 expression was observed in 87% (139/160) of patients. The distribution of TROP2 expression in post-treatment samples was analogous to that in pre-treatment samples when compared using the Wilcoxon signed-rank test (P = 0.509). However, an increase in TROP2 expression was seen in 19 (12%), and a decrease in 20 (13%) patients. Patients treated with targeted therapy showed significantly higher changes in TROP2 expression (P = 0.019) and thoracic radiotherapy was more likely to increase TROP2 expression than chemotherapy alone.Conclusion: TROP2 was expressed in most lung cancer specimens before and after anti-cancer treatments. Additionally, some anti-cancer treatments might alter the TROP2 expression. These results may provide a strong rationale for TROP2-directed therapy against advanced lung cancer.
A Fuzzy Based Improved Control Strategy of Dynamic Voltage Restorer for Low Voltage and High Voltage Ride Through Compensation for Variable Speed Hybrid Energy&nbsp;
Authors: R Uthra , D Suchitra 
Published: March 2021

Abstract
In present-day power generation systems, the integration of many renewable energy sources creating more sustainable hybrid energy network can be witnessed. The Hybrid energy system regulates the escalation in the cost of the energy that are linked with currently established energy resources. One such predominantly used Hybrid energy system happens to be Wind / Photo Voltaic (PV) integrated system which is a viable alternative to meet the rising energy demands.Fault Ride Through (FRT) is one of the cardinal essentialities that is perceived as an indispensable grid code because the effect of faults may propagate to a larger geographical area if FRT is not achieved. In a Hybrid system the ability of the generator to remain connected at the time of short electric fault like voltage dip is identified as Low Voltage Ride Through (LVRT) and voltage swell is known as High Voltage Ride Through (HVRT). When fault occurs at the point of the grid, LVRT or HVRT depending on the fault has to be determined and a pertinent compensation should be carried out. This paper proposes a compensation technique based on Dynamic Voltage Restorer (DVR) employing fuzzy controller for LVRT and HVRT in hybrid systems. The disparityin the voltage at PCC is traced and is used to inject the necessary deficit voltage in case of sag or suppress the excessive voltage in case of swell in the hybrid system by means of DVR. This compensation in turn enables the parameters like DC link and stator voltage to stay within the admissible limits in the event of fault at the point of grid.
Epidemiological Analysis of 459 Patients Hospitalized With Traumatic Spinal Cord Injury in Suzhou City, China
Authors: Niu Shengjia , Yang Weixing , Zhang Dawei , Chen Qingmei , Wang Haibo , Liu Yue , Zhou Qingqing 
Published: March 2021

Abstract
Study design Retrospective study.Objective: To analyze the epidemiological characteristics of patients hospitalized with traumatic spinal cord injury (TSCI). Setting: The First Affiliated Hospital of Soochow UniversityMethods: This retrospective epidemiological study included 459 patients hospitalized with TSCI. Results: Mean age in this patient cohort was 50.7 ± 14.2 years, and 50–59 year olds were the most vulnerable age group (27.0%). Main cause of injury was fall from height in the 10-49 year-old group, traffic accident in the 50-79 year-old group, and low fall in ≥80 year-old group. Traffic accident accounted for the largest proportion of injuries (37.0%). The male-to-female ratio was 3.1:1, with more males than females accounting for all causes of injury. The difference in proportion of males to females was the greatest for fall from height and lowest for low fall. The anatomical site most vulnerable to fracture was the cervical segment (68.2%). Thoracolumbar segment (15.0%) was the anatomical site most frequently injured in fall from height, and the cervical segment was the most commonly injured site for all the remaining causes of injury. Most patients were classified with Grade D injuries using the American Spinal Injury Association (ASIA) Impairment scale. The most common complication of injuries was urinary tract infection (48.3%). Patients with bedsores had the longest hospital stay, with an average of 105 days. Patients with complications had significantly longer hospital stays than those without complications. Patients with multiple complications had longer hospital stays than those with a single complication. Patients receiving surgery accounted for 82.4%, and those receiving conservative treatment accounted for 17.6%, of our patient population. Average length of hospital stay for all patients was 51.1 ± 79.3 days. Patients that received surgery, those with ASIA Grade A, and those with injured thoracolumbar segment had prolonged hospital stays.Conclusion: Patients with TSCI were generally of advanced age. Traffic accident was the main cause of TSCI, but causes of TSCI were different in different age groups. Elderly patients showed a high rate of low fall. The majority of TSCI occurred in male patients, and males and females had different causes of TSCI. Neck injuries were relatively common. Injury sites varied according to the different causes of injury. The prolonged hospital stays of patients with TSCI were related to numerous factors. Our study highlights the need to focus on the prevention and treatment of various complications, and selection of appropriate treatment, in patients with TSCI. TSCI should be comprehensively prevented according to its epidemiological characteristics.
Knockdown by MSH2 and EPCAM siRNA suppress Wnt/β-Catenin Pathway in HCT116 Cell Line
Authors: Juhari Wan Khairunnisa Wan , Noordin Khairul Bariah Ahmad Amin , Rahman Wan Faiziah Wan Abdul , Zakaria Andee Dzulkarnaen , Mokhter Wan Muhammad Mokhzani Wan Muhammad , Zilfalil Bin Alwi 
Published: March 2021

Abstract
Purpose: Small interfering RNA (siRNA) has the potential as a therapeutic approach against selective pathways in colorectal cancer. EPCAM, a transmembrane glycoprotein mediating cell adhesion, was known to be involved in suppressing Wnt/β-catenin pathway, an important pathway for tumour progression in colon cancer cells. EPCAM deletions caused a transcriptional read-through that may silence its neighbouring gene, MSH2. This study aimed to investigate the effect of co-siRNA targeted genes, MSH2 and EPCAM, in colon cancer cell line, HCT116, and their effect in regulating the Wnt/β-catenin pathway. Methods: Pre-designed siRNA of MSH2 and EPCAM were transfected into HCT116 cells. The cells were divided into six group of treatments: untreated cell group, cells treated with negative control siRNA, MSH2-siRNA treated cells, EPCAM-siRNA treated cells, cells treated with both EPCAM and MSH2-siRNA, and cells treated with transfection reagent (mock control). The mRNA and protein expression following the individual and combined siRNA treatments were assessed by quantitative polymerase chain reaction and Western blot. Results: The mRNA and protein expression levels of MSH2, EPCAM and β-catenin were reduced in the individual MSH2 and EPCAM-siRNA treated samples as compared to the untreated sample. Further reduction of mRNA and protein expressions for MSH2, EPCAM and β-catenin were identified in combined siRNA treatments. Conclusion: Reduction of β-catenin expression by simultaneous silencing of MSH2 and EPCAM suggested that these genes may play a role in supressing the Wnt/β-catenin pathway in cancer cells.
Personalized analysis of minimal residual cancer cells in peritoneal lavage fluid predicts peritoneal dissemination of gastric cancer
Authors: Zhao Dongbin , Yue Pinli , Wang Tongbo , Wang Pei , Song Qianqian , Wang Jingjing , jiao Yuchen 
Published: March 2021

Abstract
Peritoneal dissemination (PD) is the major type of gastric cancer (GC) recurrence and leads to rapid death. Current approach cannot precisely determine which patients are at high risk of PD. In this study, we developed a technology to detect minimal residual cancer cells in peritoneal lavage fluid (PLF) samples by parallel profiling tumor-specific mutations. We applied the technology to a prospective cohort of 110 GC patients. The technology showed ultra-high sensitivity by successfully detecting all the 27 cases that developed PD. The minimal residual cancer cells in PLF was associated with an increased risk of PD (HR = 145.13; 95%CI = 20.20-18435.79; p < 0.001) and significantly shorter overall survival. In pathologically high-risk (T4) patients, the PLF mutation profiling model exhibited even greater specificity of 91% and positive predictive value of 88%, while retaining sensitivity of 100%. PLF cancer cell fraction remained the strongest independent predictor of PD and recurrence-free survival over pathologic diagnosis and cytological diagnosis in GC patients. This approach may help in the postsurgical management of GC patients by detecting PD far before the metastatic lesions grow to significant size detectable by conventional methods such as MRI and CT scanning.
Development and Psychometric Testing of an Oral Presentation Scale for Nursing Students
Authors: CHIANG Yi-Chien , LEE Hsiang-Chun , CHU Tsung-Lan , Wu Chia-Ling , HSIAO Ya-Chu 
Published: March 2021

Abstract
Background: Oral presentations are an important educational component for nursing students. However, there are no reliable tools for objective evaluations of presentations. We aimed to develop a measurement scale for nursing students’ oral presentations and evaluate its reliability and validity. Methods: A literature review and face-to-face interviews with university tutors in oral presentations and students generated 28 scale items. The validity and reliability of the scale was evaluated with exploratory and confirmatory factor analysis, criterion-related validity, internal consistency, and test-retest reliability. Results: Nursing students provided data for exploratory factor analysis (n = 325), which resulted in 20 items. Three factors explained 64.75% of the total variance: accuracy of content, effective communication, and clarity of speech. The Cronbach's α value was .94 for the total scale and the three factors ranged from .84 to .93. Construct validity was examined with confirmatory factor analysis using data from another 325 students. Five items were deleted, and fit indices of the model were acceptable, with the exception of the adjusted goodness of fit index, which was below the minimum criteria. The final 15-item oral presentation scale (OPS) was significantly correlated with the Personal Report of Communication Apprehension scale (r = -.51, p < .001) and Self-Perceived Communication Competence Scale (r = .45, p < .001) indicating excellent criterion-related validity. The intra-class correlation coefficient for a 3-week test-retest (r = .681, p < .001) indicated the OPS was stable over time. Conclusions: The OPS could be adopted to help nursing programs prepare for and improve students’ oral presentations.
Hospital to Home: Supporting the Transition from Hospital to Home for Older Adults
Authors: Barber Brittany , Weeks Lori , Steeves-Dorey Lexie , McVeigh Wendy , Stevens Susan , Moody Elaine , Warner Grace 
Published: March 2021

Abstract
Background: An increasing proportion of older adults experience avoidable hospitalizations, and some are potentially entering long-term care homes earlier and often unnecessarily. Within Canada, approximately 10 percent of newly admitted long-term care residents could have potentially been cared for at home. Without adequate support from health care services to transition older adults from hospital to home, they often lack access to appropriate services when they are needed in the community thus making them more vulnerable to avoidable hospital visits, rapid deterioration of health, and earlier and unnecessary placement into residential long-term care. The purpose of this study was to collaborate with an existing enhanced home care program called Home Again in Halifax, Nova Scotia, to identify factors that contribute to older adult patients being assessed as requiring long-term care when they could potentially return home with enhanced supports. Methods: Through a retrospective case study design, we analyzed nine interviews for three focal patient cases including older adult patients, their family or friend caregivers, and healthcare professionals. Results: Findings indicate home care services for older adults are being sought too late, after hospital re-admission, or a rapid decline in health status when family caregivers are already experiencing caregiver burnout. Limitations in home care services ultimately led to barriers preventing family caregivers from continuing to care for older adults at home, such as absence of overnight services, a lack of information about home care services, and a lack of knowledge to navigate resources available. Conclusion: This study contributes knowledge about gaps within home care and transitional care services, highlighting the significance of investing in additional home care services for rehabilitation and the prevention of rapidly deteriorating health when older adults are discharged home after their first hospital visit. By understanding experiences and challenges of patients, family or friend caregivers, and healthcare professionals, we identified ways to reduce healthcare costs and improve the delivery and quality of home care services to better support older adult patients and their family or friend caregivers and to ensure hospitalized older adults are not unnecessarily admitted to nursing homes.
Earlier Corneal Topographical Changes After Different Pterygium Surgical Techniques: a Comparative Pentacam Scheimpflug Imaging Study
Authors: İpek Şefik Can , Yilmaz Yusuf Cem , Gobeka Hamidu Hamisi , Özer Muhammet Derda 
Published: March 2021

Abstract
Purpose: To investigate earlier corneal topographical changes and visual acuity after different pterygium surgical techniques using the Pentacam Scheimpflug imaging system.Methods: This study enrolled 98 patients with unilateral primary nasal pterygium. Pterygium surgery with either a conjunctival autograft (group 1) or an anchored conjunctival rotational flap (group 2), with fibrin tissue adhesive was performed under topical anesthesia. Baseline and one-month post-operative best-corrected visual acuity (BCVA) in logarithm of the Minimum Angle of Resolution (logMAR) and anterior corneal astigmatism (ACA), flat keratometry (Kf), steep keratometry (Ks) and posterior corneal astigmatism (PCA) were analyzed.Results: Mean BCVA improved from baseline 0.119±0.113 to 0.082±0.086 logMAR in group 1, and from baseline 0.169±0.128 to 0.120±0.121 logMAR in group 2. There were no statistically significant differences between the two groups in pre-and post-operative ACA, PCA, Kf and Ks. (p=0.686; 0.9020.107; and 0.592, respectively). Intra-group analysis revealed statistically significant differences in both groups: ACA (p<0.001 for both groups), with a greater difference in group 1 (2.072), and Kf (p<0.001 for both groups), with a slightly greater change in group 2 (1.910). While there were no statistically significant Ks intra-group differences in both groups (group 1: p=0.091; group 2: 0.092), group 1 (0.0522) displayed more Ks changes. There were also no statistically significant intra-group PCA differences in both groups (p=0.061 for both groups). However, Group 2 (-0.020) displayed greater changes.Conclusions: Significantly improved anterior corneal topographical changes highly associated with conjunctival autografting. However, the conjunctival rotational flap was associated with relatively greater posterior corneal topographical changes.
Correlation Between Expression levels of lncRNA FER1L4 and RB1 in Patients with Colorectal Cancer
Authors: Ostovarpour Marjan , Khalaj-Kondori Mohammad , Ghasemi Tayyebeh 
Published: March 2021

Abstract
Colorectal cancer is one of the most common cancers in the world. Studies demonstrated that lncRNA FER1L4 was downregulated in different cancers and its expression was positively correlated with RB1 in a competing endogenous RNAs network. We investigated expression levels of FER1L4 lncRNA and RB1 in patients with colorectal cancer. 50 paired colorectal tumor and non-tumor marginal tissues, as well as 30 paired adenomatous colorectal polyps and matched adjacent normal tissues were obtained from the patients. Total RNA was extracted from the samples and cDNAs were synthesized. Their expression were quantified by qRT-PCR. Correlation between FER1L4 and RB1 expression levels was analyzed by Pearson correlation test. Finally, ROC curve analysis was used to evaluate their biomarker potency. We observed significant downregulation of FER1L4, but upregulation of RB1 in the colorectal tumors compared with non-tumor as well as with the adenomatous colorectal polyp tissues. However, RB1 expression was positively correlated with the FER1L4 expression both in the tumor and polyp samples. ROC curve analysis showed that both FER1L4 and RB1 expression levels could discriminate tumor from non-tumor and tumor from polyp samples. None of clinicopathological characteristics of patients were associated with FER1L4 or RB1 expression levels. Despite downregulation of FER1L4 and upregulation of RB1 in tumors compared with non-tumor tissues, the expression of RB1 was positively correlated with the expression of FER1L4 in the colorectal tumor as well as in the adenomatous colorectal polyp tissues. FER1L4 expression level might be considered as a potential biomarker for colorectal cancer development.
Decrease of chemerin by aerobic exercise improved glycolipid metabolism of diabetes through increasing key metabolism enzymes and protein mediated by PPARγ
Authors: Lin Xiaojing , Yin Lijun , Qu Jing , Wang Xiaohui 
Published: March 2021

Abstract
Background: Our previous work indicated that exercise-induced reduction of chemerin (an adipokine and chemokine) played an important role in the improvement of glycolipid metabolism of diabetic rats, and this manuscript are to: (1) clarify peroxisome proliferator-activated receptor (PPARγ) mediating the regulation of decreased chemerin on glycolipid metabolism key enzymes (including adipose triglyceride lipase (ATGL), lipoprotein lipase (LPL), glucose transporter 4 (GLUT4) and phosphoenolpyruvate carboxykinase (PEPCK)) in diabetic rats; (2) demonstrate the crucial role of decreased chemerin on exercise-induced improvement of glycolipid metabolism in diabetic mice by exogenous chemerin supplement (for only mice-resource chemerin available). Methods: After establishing type 2 diabetes models, diabetic SD rats were randomly divided into 4 groups: diabetes (DM), exercised diabetes (EDM), EDM plus PPARγ agonist pioglitazone (EDP), and EDM plus PPARγ antagonist GW9662 (EDG), while diabetic ICR mice divided into 3 groups: DM, EDM and EDM plus exogenous chemerin supplementation (EDC). The exercised rodents participated in 4-week (rats) or 6-week (mice) moderate-intensity aerobic exercise, and at 30 min before exercise pioglitazone (10 mg/kg) and GW9662 (1 mg/kg) were intragastrically administered to EDP and EDG rats respectively, while recombinant mouse chemerin (8 µg/kg) was intraperitoneally injected to C and EDC mice. Glycolipid metabolism indexes were determined. Serum chemerin and the protein levels of the above molecules in metabolic organs (liver, gastrocnemius and epididymal fat) were detected by ELISA and Western blot, respectively. Results: (1) In diabetes rats, aerobic exercise-induced increases of ATGL and LPL (livers and gastrocnemius) as well as decrease of PEPCK (livers) were reversed by GW9662, and further strengthened by pioglitazones. (2) In diabetes mice, aerobic exercise also significantly increased the levels of PPARγ, ATGL, LPL and GLUT4 as well as decreased the level of PEPCK; furthermore, the changes of the above molecules and improvements of glycolipid metabolism and fatty liver were partly reversed by exogenous chemerin. Conclusion: The decreased chemerin played important roles in aerobic exercise-induced improvements of glycolipid metabolism and fatty liver in diabetes, through increasing glycolipid metabolism key enzymes and protein (ATGL, LPL and GLUT4) mediated by PPARg.
The malaria burden of Amerindian groups of three Venezuelan states: A descriptive study based on programmatic data
Authors: Gabaldon-Figueira Juan Carlos , Chaccour Carlos , Moreno Jorge E , Villegas Maria , Villegas Leopoldo 
Published: March 2021

Abstract
Background: Fifty-three percent of all cases of malaria in the Americas in 2019 came from Venezuela, where the epidemic is heavily focused south of the Orinoco river, and where most of the country’s Amerindian groups live. Despite the disease is known to represent a significant public health problem among these populations, little epidemiological data exists on the subject. This study aims to provide information on malaria incidence, geospatial clustering, and risk factors associated to Plasmodium falciparum infection among these groups.Methods: This is a descriptive study based on the analysis of published and unpublished programmatic data collected by Venezuelan health authorities and non-government organizations between 2014 and 2018. The Annual Parasite Index among indigenous groups (API-i) in municipalities of three states: Amazonas, Bolivar and Sucre was calculated and compared using the Kruskal Wallis test, risk factors for P. falciparum infection were identified via binomial logistic regression and maps were constructed to identify clusters of malaria cases among indigenous patients via Moran’s I and Getis-Ord’s hot spot analysis.Results: 116,097 cases of malaria in Amerindian groups were registered during the study period. An increasing trend was observed between 2014 and 2016 but reverted in 2018. Malaria incidence remains higher than in 2014 and hot spots were identified in the three states, although more importantly south of Bolivar. Most cases (73.3%) were caused by P. vivax, but the Hoti and Yanomami indigenous groups presented higher odds for infection with P. falciparum.Conclusion: Malaria cases among Amerindian populations increased between 2014 and 2018 and seem to have a different geographic distribution as those among the general population. Our findings suggest that tailored, cost-effective interventions will be needed to curb the negative impact of malaria transmission in these groups. Attention to the Hoti and Yanomami should be prioritized.