bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wnt target enhancer regulation by a CDX/TCF transcription factor collective and a novel DNA motif
Authors: Ramakrishnan Aravinda-Bharathi , Chen Lisheng , Burby Peter , Cadigan Ken M. 
Published: January 2021

AbstractTranscriptional regulation by Wnt signalling is primarily thought to be accomplished by a complex of β-catenin and TCF family transcription factors (TFs). Although numerous studies have suggested that additional TFs play roles in regulating Wnt target genes, their mechanisms of action have not been investigated in detail. We characterised a Wnt-responsive element (WRE) downstream of the Wnt target geneAxin2and found that TCFs and Caudal-related homeodomain (CDX) proteins were required for its activation. Using a new separation-of-function TCF mutant, we found that WRE activity requires the formation of a TCF/CDX complex. Our systematic mutagenesis of this enhancer identified other sequences essential for activation by Wnt signalling, including several copies of a novel CAG DNA motif. Computational and experimental evidence indicates that the TCF/CDX/CAG mode of regulation is prevalent in multiple WREs. Put together, our results demonstrate the complex nature of cis- and trans- interactions required for signal-dependent enhancer activity.
REPP: A robust cross-platform solution for online sensorimotor synchronization experiments
Authors: Anglada-Tort Manuel , Harrison Peter M. C. , Jacoby Nori 
Published: January 2021

AbstractSensorimotor synchronization (SMS), the rhythmic coordination of perception and action, is a fundamental human skill that supports many behaviors, from daily repetitive routines to the most complex behavioural coordination, including music and dance (Repp 2005; Repp & Su, 2013). Research on SMS has been mostly conducted in the laboratory using finger tapping paradigms, where participants typically tap with their index finger to a rhythmic sequence of auditory stimuli. However, these experiments require equipment with high temporal fidelity to capture the asynchronies between the time of the tap and the corresponding cue event. Thus, SMS is particularly challenging to study with online research, where variability in participants’ hardware and software can introduce uncontrolled latency and jitter into recordings. Here we present REPP (Rhythm ExPeriment Platform), a novel technology for measuring SMS in online experiments that can work efficiently using the built-in microphone and speakers of standard laptop computers. The audio stimulus (e.g., a metronome or a music excerpt) is played through the speakers and the resulting signal is recorded along with participants’ responses in a single channel. The resulting recording is then analyzed using signal processing techniques to extract and align timing cues with high temporal accuracy. In this paper, we validate REPP through a series of calibration and behavioural experiments. We demonstrate that our technology achieves high temporal accuracy (latency and jitter within 2 ms on average), high test-retest reliability both in the laboratory (r= .87) and online (r= .80), and high concurrent validity (r= .94). We also show that REPP is fully automated and customizable, enabling researchers to monitor experiments in real time and to implement a wide variety of SMS paradigms. We discuss methods for ensuring high recruiting efficiency and data quality, including pre-screening tests and automatic procedures for quality monitoring. REPP can therefore open new avenues for research on SMS that would be nearly impossible in the laboratory, reducing experimental costs while massively increasing the reach, scalability and speed of data collection.
Fluorescent Glycan Fingerprinting of SARS2 Spike Proteins
Authors: Wu Zhengliang L , Ertelt James M 
Published: January 2021

AbstractGlycosylation is the most common post-translational modification and has myriad biological functions. However, glycan analysis and research has always been a challenge. Here, we would like to present new techniques of glycan fingerprinting based on enzymatic fluorescent labeling and gel electrophoresis. The method is illustrated on SARS-2 spike (S) glycoproteins. SARS-2, a novel coronavirus and the causative agent of COVID-19 pandemic, has devastated the world since the end of 2019. To obtain the N-glycan fingerprint of a S protein, glycans released from the protein are first labeled through enzymatic incorporation of fluorophore-conjugated sialic acid or fucose, and then separated on acrylamide gel through electrophoresis, and finally visualized with a fluorescent imager. To identify the labeled glycans of a fingerprint, glycan standards and glycan ladders that are enzymatically generated are run alongside the samples as references. By comparing the mobility of a labeled glycan to that of a glycan standard, the identity of glycans maybe determined. Due to lack of enzyme for broad O-glycans releasing, O-glycans on the RBD protein are labeled with fluorescent sialic acid and digested with trypsin to obtain labeled glycan peptides that are then separated on gel. Glycan fingerprinting could serve as a quick way for global assessment of the glycosylation of a glycoprotein.
Altered temporal sequence of transcriptional regulators in the generation of human cerebellar granule cells
Authors: Behesti Hourinaz , Kocabas Arif , Buchholz David E. , Carroll Thomas S. , Hatten Mary E. 
Published: January 2021

SummaryBrain development is regulated by conserved transcriptional programs across species, but little is known about divergent mechanisms that create species-specific characteristics. Among brain regions, the cerebellum is now recognized to contribute to human cognitive evolution having a broad range of non-motor cognitive functions in addition to motor control. Emerging studies highlight the complexity of human cerebellar histogenesis, compared with non-human primates and rodents, making it important to develop methods to generate human cerebellar neurons that closely resemble those in the developing human cerebellum. Here we report a rapid and simple protocol for the directed derivation of the human ATOH1 lineage, the precursor of excitatory cerebellar neurons, from human pluripotent stem cells (hPSC), and strategies to decrease culture variability; a common limitation in hPSC studies. Upon transplantation into juvenile mice, early postmitotic hPSC-derived cerebellar granule cells migrated along glial fibers and integrated into the cerebellar cortex. By Translational Ribosome Affinity Purification (TRAP)-seq, the ATOH1 lineage most closely resembled human cerebellar tissue in the second trimester. Unexpectedly, TRAP-seq identified a heterochronic shift in the expression of RBFOX3 (NeuN) and NEUROD1, which are classically associated with differentiated neurons, within granule cell progenitors (GCPs) in the human external granule layer. This molecular divergence may provide the mechanism by which the GCP pool persists into year two post birth in humans, but only lasts for two weeks in mice. Our approach provides a scalablein vitromodel of the human ATOH1 lineage that yields cerebellar granule cells within 48 days as well as a strategy for identifying uniquely human cellular and molecular characteristics.
Hydrophobic residues advance the onset of simple coacervation in intrinsically disordered proteins at low densities: Insights from field theoretical simulations studies
Authors: Ramanjanappa Satwik , Iyer Sahithya S. , Srivastava Anand 
Published: January 2021

AbstractIntrinsically disordered proteins (IDPs) have engendered a definitive change in the way we think about the classical “sequence-structure-function” dogma. Their conformational pliability and rich molecular recognition features endow them with the ability to bind to diverse partners and predispose them to an elaborate functional armory. And of late, with studies on IDP-based liquid-liquid phase separation (LLPS) leading to formation of functional subcellular coacervates - best described as “membrane-less organelles (MLOs)”, IDPs are also bringing about paradigmatic changes in the way we think about biomolecular assemblies and subcellular organization. Though it is well recognized that the phase behavior of a given IDP is tightly coupled to its amino-acid sequences, there are only a few theories to model polyampholyte coacervation for IDPs. Recently, Joan-Emma Shea and co-workers used field theoretical simulations (FTS) to elucidate the complete phase diagram for LLPS of IDPs by considering different permutations of 50-residues chain representing 25 Lysine and 25 Glutamic acid [1]. Our work is an extension of that FTS framework where we develop and solve an augmented Hamiltonian that also accounts for hydrophobic interactions in the chain. We show that incorporation of hydrophobic interactions result in an advanced onset of coacervation at low densities. The patterning of hydrophobic, positive and negative residues plays important role in determining relative differences in the onset of phase separation. Though still very coarse-grained, once additional chemical specificities are incorporated, these high throughput analytical theory methods can be used as a starting point for designing sequences that drive LLPS.
The trade policy response to COVID-19 and its implications for international business
Authors: Curran Louise , Eckhardt Jappe , Lee Jaemin 
Published: January 2021

Purpose
This paper aims to explore trade policy measures taken in response to COVID-19 and analyses in detail their extent and nature. It assesses their compatibility with World Trade Organisation (WTO) agreements: specifically, whether they were necessary and justifiable efforts to protect the security and health of populations and asks how this widespread recourse to trade barriers may impact on international business?

Design/methodology/approach
This paper analyses an extensive database from the International Trade Centre of trade measures taken in response to COVID-19. It differentiates by type of country, nature and coverage of measures (imports or exports, type of product…). On the basis of existing jurisprudence, this paper analyses whether restrictive measures were likely to be judged legal under WTO rules.

Findings
This paper finds that, although the majority of trade measures are probably justifiable, there were nevertheless many measures whose coverage and/or nature was such that a justification under existing WTO exceptions is, at the very least, arguable. Such widespread and intense instigation of potentially WTO incompatible measures in such a short period of time undoubtedly undermines the global trade rules on which international business has relied for decades.

Originality/value
There is little existing analysis of the legality of measures taken under the security exceptions and no substantial analyses of the measures taken in response to COVID-19. Furthermore, little scholarly attention has been paid to the impacts on international business of the increasing use of WTO “exceptions” to justify trade measures to protect national industries and populations.
Looking in the mirror: the global '68 through the Brazilian daily press
Authors: Hernández Huerta José Luis 
Published: January 2021

PurposeThis article explains the process of construction and configuration of the Brazilian social imaginary on the global '68 using the daily press as source material.Design/methodology/approachIt looks at the narratives conveyed by the press about the condition, situation, motivations, aspirations and capacity for action of young university students. The analysis is focused mainly on the usage of totalitarian language and permits an in-depth view of the reality of life in Brazil at the time and the role played by the students in the resistance to the dictatorship. It also includes an analysis of how other students' protests of 1968 – in Poland and Mexico – were portrayed through the media, and how they helped to shape the collective imaginary about Brazilian university students, situating it in a conjuncture of broader dimensions and connections.FindingsThe youth of Brazil, Poland and Mexico were represented as active political and social subjects, capable of defying, and sometimes profoundly upsetting, the established order. Violence and the discourse of violence were constant unifying elements in the narratives created by the daily press. This helped generate an image of university students which portrayed them as a rebellious, revolutionary and/or subversive sector of the population, responsible for one of the most extensive and profound social and political crises which those countries had experienced in decades.Originality/valueThis is the first study of the Brazilian reception of the '68 Polish and Mexican students' protest and its implications for the social narrative of students' resistance in Brazil.
Developing smart community based on information and communication technology: an experience of Kemaman smart community, Malaysia
Authors: Mohd Satar NurulHuda , Saifullah Md. Khaled , Masud Muhammad Mehedi , Kari Fatimah Binti 
Published: January 2021

PurposeIn light of the rapid evolution of information and communication technology (ICT), every society is faced with many issues such as social exclusion, inequality and the digital divide. Hence, there is need to solve these complex challenges without comprising any development objective. A practical solution in this regard includes establishment of a sustainable model of community development. Therefore, this paper aims to identify the role of education in promoting the awareness on the use of ICT-based infrastructure among the general public to enhance their socioeconomic status. In addition, this study sets out to establish the nexus between socioeconomic status, ICT programme as well as the awareness of Kemaman Smart Community (KSC) development project.Design/methodology/approachThis paper adopted a quantitative approach based on data collected through a survey on information literacy in KSC. The logit behavioural model and structural equation model were adopted as a quantitative technique to verify the objective of the study.FindingsThe result of the logit behavioural model showed that education, income and age have a positive impact on the KSC programme awareness. The SEM models revealed that income level and education (especially at the tertiary level) significantly enhance the awareness of KSC project. On the contrary, poverty remains regressive towards the community awareness of KSC programmessPractical implicationsInformation literacy and knowledge-based programme promote a more inclusive development approach. Moreover, education, socioeconomic status, information literacy and knowledge-based programme are essential to develop a smart community towards achieving a high-income nation status. Future replication of smart community project in other parts of Malaysia may need to consider these factors for a comprehensive community development strategy.Originality/valueKSC is the first smart city initiative by the government of Malaysia. Besides, primary data were used in this study. The findings of the study will provide academics and policymakers a better understanding of the smart city initiative.
A method of locating the 3D centers of retroreflectors based on deep learning
Authors: Zhang BinBin , Zhang Fumin , Qu Xinghua 
Published: January 2021

Purpose
Laser-based measurement techniques offer various advantages over conventional measurement techniques, such as no-destructive, no-contact, fast and long measuring distance. In cooperative laser ranging systems, it’s crucial to extract center coordinates of retroreflectors to accomplish automatic measurement. To solve this problem, this paper aims to propose a novel method.

Design/methodology/approach
We propose a method using Mask RCNN (Region Convolutional Neural Network), with ResNet101 (Residual Network 101) and FPN (Feature Pyramid Network) as the backbone, to localize retroreflectors, realizing automatic recognition in different backgrounds. Compared with two other deep learning algorithms, experiments show that the recognition rate of Mask RCNN is better especially for small-scale targets. Based on this, an ellipse detection algorithm is introduced to obtain the ellipses of retroreflectors from recognized target areas. The center coordinates of retroreflectors in the camera coordinate system are obtained by using a mathematics method.

Findings
To verify the accuracy of this method, an experiment was carried out: the distance between two retroreflectors with a known distance of 1,000.109 mm was measured, with 2.596 mm root-mean-squar error, meeting the requirements of the coarse location of retroreflectors.

Research limitations/implications
The research limitations/implications are as follows: (i) As the data set only has 200 pictures, although we have used some data augmentation methods such as rotating, mirroring and cropping, there is still room for improvement in the generalization ability of detection. (ii) The ellipse detection algorithm needs to work in relatively dark conditions, as the retroreflector is made of stainless steel, which easily reflects light.

Originality/value
The originality/value of the article lies in being able to obtain center coordinates of multiple retroreflectors automatically even in a cluttered background; being able to recognize retroreflectors with different sizes, especially for small targets; meeting the recognition requirement of multiple targets in a large field of view and obtaining 3 D centers of targets by monocular model-based vision.
Repositioning luxury fashion brands as intentional agents: the roles of consumer envy and admiration
Authors: Joo Bo Ra , Kim Hye-Young 
Published: January 2021

PurposeBuilding on the Brands as Intentional Agents Framework (BIAF), the aim of this study is to demonstrate the effectiveness of social media marketing (SMM) as a tool to communicate luxury fashion brands' good intentions toward the general public.Design/methodology/approachA sample of 488 US female consumers was used to test a conceptual model delineating the sequential linkages from luxury fashion brands' intentions to brand emotions (i.e. envy vs admiration) and to consumer–brand relationships (i.e. emotional brand attachment and brand forgiveness). Structural equation modeling (SEM) was performed to test the measurement and structural models.FindingsThe results indicated that luxury fashion brands' “populist” intentions had a positive impact on consumer admiration. Both consumer envy and admiration had positive effects on emotional brand attachment and brand forgiveness. However, admiration had a stronger effect than envy on these relational consumer responses.Originality/valueThis study identified that luxury fashion brands, frequently stereotyped as exclusive, can become brands admired by mass-market consumers by expressing warmth on social media. Drawing on social psychological perspectives and the BIAF, this study adds to the literature on luxury brands' social media communication by demonstrating the effectiveness of brand warmth to induce consumers' strong relational outcomes.
Traumatic stress sufferers: work as therapy or trigger?
Authors: Williams Scott , Williams Jonathan 
Published: January 2021

PurposeWhile a return to work following trauma exposure can be therapeutic, this is not always so. As with many topics related to traumatic stress in organizations, several contingency factors complicate the effort to draw an overarching conclusion about whether returning to work is therapeutic. The purpose of this paper is to present important determinants of whether work is therapeutic or triggering for those with traumatic stress conditions. The need for contingency approaches in the study of traumatic stress in organizations is illustrated.Design/methodology/approachLiterature on traumatic stress in organizations is reviewed.FindingsThree of the key determinants of whether a return to work is therapeutic or triggering for traumatic stress sufferers are trauma-type contingencies, condition-type contingencies and work-setting contingencies. For instance, human-caused and task-related traumas are more likely than natural disasters to make a return-to-work triggering. Additionally, the time since developing a traumatic stress condition is inversely related to the degree of improvement in that condition through the experience of working. Moreover, managerial actions can affect how therapeutic an employee’s return to work is.Practical implicationsThese findings suggest the challenges of reintegrating a traumatized employee to the workplace can be highly situation-specific. Careful consideration of the traumatic event suffered by each traumatic stress victim, their traumatic stress condition, and the work setting to which they would return are recommended.Social implicationsPromoting mental health in organizations can contribute to employers’ social performance.Originality/valueExamination of the factors that complicate predicting whether work is therapeutic posttrauma demonstrates how contingency approaches can advance research on trauma in organizations.
Insecurity and turnover as robots take charge: impact of neuroticism and change-related uncertainty
Authors: Priyadarshi Pushpendra , Premchandran Rajesh 
Published: January 2021

PurposeData were collected using a survey questionnaire of 379 participants from business process outsourcing (BPO) organizations affected by robotic process automation (RPA). Structural equation modelling and hierarchical regression analysis were used to test the hypothesized relationships between the study variables.Design/methodology/approachThe purpose of this paper is to develop and test a moderated-mediation model examining the relationships between uncertainty around changes due to RPA, neuroticism and job insecurity, and turnover intentions among BPO employees in India.FindingsUncertainty around RPA and neuroticism cause job insecurity among employees resulting in their intent to quit the organization. Further, the impact of job insecurity is influenced by employees' commitment to automation. Outlining the ways in which RPA-driven change impacts employees and organizations, our findings underscore the need for upskilling the affected employees besides developing coping mechanisms as a buffer to the negative impacts of large-scale automation-driven transformation in the industry under study.Originality/valueAmidst the debate around the impact of RPA in developing countries, our research is the first attempt to systematically examine how RPA has led to concerns around job security leading to turnover intention among employees in the Indian BPO sector. It uniquely highlights the role of personality besides the issue of growing uncertainty due to RPA, requiring the immediate attention of organizations.