bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple one-step electrochemical deposition of bioinspired nanocomposite for the non-enzymatic detection of dopamine
Authors: Kathiresan Vijayaraj , Thirumalai Dinakaran , Rajarathinam Thenmozhi , Yeom Miri , Lee Jaewon , Kim Suhkmann , Yoon Jang-Hee , Chang Seung-Cheol 
Published: February 2021

AbstractA simple and cost-effective electrochemical synthesis of carbon-based nanomaterials for electrochemical biosensor is of great challenge these days. Our study describes a single-step electrochemical deposition strategy to prepare a nanocomposite of electrochemically reduced graphene oxide (ErGO), multi-walled carbon nanotubes (MWCNTs), and polypyrrole (PPy) in an aqueous solution of pH 7.0 for dopamine (DA) detection. The ErGO/MWCNTs/PPy nanocomposites show enhanced electrochemical performance due to the strong π–π* stacking interactions among ErGO, MWCNTs, and PPy. The efficient interaction of the nanocomposites is confirmed by evaluating its physical and electrochemical characteristics using field-emission scanning electron microscopy, Raman spectroscopy, electrochemical impedance spectroscopy, cyclic voltammetry, and amperometry. The deposited nanocomposites are highly stable on the substrates and possess high surface areas, which is vital to improve the sensitivity and selectivity for DA detection. The controlled deposition of the ErGO/MWCNTs/PPy nanocomposites can provide enhanced electrochemical detection of DA. The sensor demonstrates a short time response within 2 s and is a highly sensitive approach for DA detection with a dynamic linear range of 25–1000 nM (R2 = 0.999). The detection limit is estimated to be 2.3 nM, and the sensor sensitivity is calculated to be 8.96 μA μM−1 cm−2, with no distinct responses observed for other biological molecules.
Development of rapid advice guideline and standard and continuous updating guideline: experiences and practice
Authors: Jin Ying-Hui , Yao Xiao-Mei , Zeng Xian-Tao 
Published: February 2021

AbstractWe published rapid advice guidelines and updated guidelines for coronavirus disease 2019 (COVID-19) management on February 6, 2020, and September 4, 2020, respectively. These two guidelines vary widely in their developmental background, type of evidence, grade of recommendation and so on. We shared our experience for the development of these two guidelines to help clinical practitioners better understand and implement guidelines and to help guideline developers facilitate communication and discussion for guideline development during the pandemic.
A qualitative exploration of the patient experience of erosive and non-erosive hand osteoarthritis
Authors: Panter Charlotte , Berry Pamela , Chauhan Deven , Fernandes Sofia , Gatsi Sally , Park Josephine , Wells Jane R. , Arbuckle Rob 
Published: February 2021

Abstract
Background
Many patients with hand osteoarthritis (HOA) experience reduced health-related quality of life. This study sought to better understand the disease and treatment experience of individuals with HOA, explore any differences in experiences between erosive and non-erosive HOA sub-types, and evaluate content validity of the Michigan Hand Outcomes Questionnaire (MHQ) in HOA.

Methods
Thirty subjects from the United States (n = 15 erosive HOA; n = 15 non-erosive HOA) participated in semi-structured interviews: concept elicitation explored symptoms/impacts important to patients; cognitive interviews assessed understanding and relevance of the MHQ. A sub-sample participated in real-time data capture (RTDC) activities via a smartphone/tablet app over 7 days. Verbatim transcripts were coded using Atlas.ti software and thematically analyzed. Concept saturation and MHQ content validity were evaluated.

Results
Most participants reported experiencing pain, swelling and stiffness, symptoms that most commonly had a direct impact on physical functioning. Substantial impacts on activities of daily living, emotional functioning, sleep and work were also reported. RTDC findings corroborated concept elicitation findings. There were no notable differences between erosive and non-erosive HOA, except nodules were reported more frequently in erosive disease. Most participants used analgesic treatments, but effects were short-lived. Pain was the symptom most frequently reported as most bothersome and important to treat. Concept saturation was achieved. MHQ items and instructions were well understood and relevant to most participants; stiffness and swelling were reported as important symptoms not included in the MHQ.

Conclusions
This study characterizes key symptoms of HOA which are burdensome for patients and not well controlled by current therapies, highlighting an unmet treatment need. Although the study is limited by a small sample size that may not be representative of the broader erosive and non-erosive HOA population, concept saturation was achieved, and our findings suggest that disease experience is similar for patients with erosive and non-erosive HOA. Evaluation of stiffness and swelling items in conjunction with the MHQ may enhance relevance and improve measurement precision to assess important domains of HQRoL in an HOA population.
Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides
Authors: Urbizo-Reyes U. C. , Aguilar-Toalá J. E. , Liceaga A. M. 
Published: February 2021

AbstractRising consumer concerns with synthetic drugs to treat non-communicable diseases (NCDs) have promoted a shift towards using natural biological active constituents that offer similar health benefits. Hairless canary seed (Phalaris canariensis L) is an emerging crop traditionally used in Mexico to treat NCDs. Peptides liberated during simulated digestion of canary seed protein are believed to be responsible for their biological activity; however, no studies have shown the effect of controlled protein hydrolysis using commercial proteases on canary seed protein’s biological activity. Therefore, this study aimed to explore the in vitro antihypertensive, antidiabetic, and anti-obesity activity of canary seed peptides derived from proteolysis with Alcalase®. Protein fractions were primarily composed of prolamins (54.07 ± 1.8%), glutelins (32.19 ± 3.18%), globulins (5.97 ± 0.52%) and albumins (5.97 ± 0.52%). The < 3 kDa and 3–10 kDa peptide fractions showed the highest inhibition capacity (p< 0.05) towards angiotensin-converting enzyme (IC50= 0.028–0.032 mg/mL) lipase (IC50= 2.15–2.27 mg/mL), α-glucosidase (IC50= 0.82–1.15 mg/mL), and dipeptidyl-peptidase-IV (IC50= 1.27–1.60 mg/mL). Additionally, these peptide fractions showed high antioxidant activity against DPPH (134.22–150.66 μmol TE/mg) and ABTS (520.92–813.33 μmol TE/mg). These results provide an insight into the potential development of functional foods using commercial enzymatic hydrolysis of canary seed proteins for treating hypertension, type-2 diabetes, and obesity.
Chemotherapy Remote Care Monitoring Program: Integration of SMS Text Patient-Reported Outcomes in the Electronic Health Record and Pharmacist Intervention for Chemotherapy-Induced Nausea and Vomiting
Authors: Hough Shannon , McDevitt Rachel , Nachar Victoria R. , Kraft Shawna , Brown Anna , Christen Catherine , Frame David , Smerage Jeffrey B. 
Published: February 2021

PURPOSE: Chemotherapy-induced nausea and vomiting (CINV) is a common cause of unplanned healthcare utilization. The University of Michigan Rogel Cancer Center initiated the chemotherapy remote care monitoring program (CRCMP) to proactively identify patients experiencing CINV and intervene before the need for urgent evaluation. METHODS: High-risk patients for CINV are identified by neurokinin-1 (NK-1) antagonist administration, enrolled in the CRCMP, and received a daily text message survey for 7 days after chemotherapy administration to report symptoms. Responses above a set threshold trigger a message to the team pharmacist for intervention. The primary outcome of 14-day unplanned healthcare use was evaluated before and after CRCMP implementation. RESULTS: In 8 months, 652 patients received an NK-1 antagonist (2,244 cycles) and 387 patients were enrolled in the CRCMP (59%). Text message response rate was 94%. Clinical pharmacists provided 248 interventions in 121 patient episodes meeting threshold criteria. Fourteen-day unplanned healthcare use was decreased in the CRCMP-enrolled NK-1 episodes (6.68% v 4.53%, P = .02). Admissions were numerically lower for those enrolling in CRCMP when only admissions for nausea were considered (0.63% v 0.35%, P = .33). CONCLUSION: The CRCMP allowed for real-time management of patient-reported CINV symptom burden based on patient-reported outcomes (PROs) and an electronic medical record-integrated SMS text questionnaire. Clinical pharmacists were key team members to manage patient symptoms. Enrollment in CRCMP significantly reduced overall unplanned healthcare utilization. Although these changes were numerically small, any reduction in unnecessary care utilizing PROs can contribute to high-value care for patients with cancer.
A lamin A/C variant causing striated muscle disease provides insights into filament organization
Authors: Kronenberg-Tenga Rafael , Tatli Meltem , Eibauer Matthias , Wu Wei , Shin Ji-Yeon , Bonne Gisèle , Worman Howard J. , Medalia Ohad 
Published: February 2021

ABSTRACT
The LMNA gene encodes the A-type lamins, which polymerize into ∼3.5-nm-thick filaments and, together with B-type lamins and associated proteins, form the nuclear lamina. Mutations in LMNA cause a wide variety of pathologies. In this study, we analyzed the nuclear lamina of embryonic fibroblasts from LmnaH222P/H222P mice, which develop cardiomyopathy and muscular dystrophy. Although the organization of the lamina appeared unaltered, there were changes in chromatin and B-type lamin expression. An increase in nuclear size and consequently a relative reduction in heterochromatin near the lamina allowed for a higher resolution structural analysis of lamin filaments using cryo-electron tomography. This was most apparent when visualizing lamin filaments in situ and using a nuclear extraction protocol. Averaging of individual segments of filaments in LmnaH222P/H222P mouse fibroblasts resolved two polymers that constitute the mature filaments. Our findings provide better views of the organization of lamin filaments and the effect of a striated muscle disease-causing mutation on nuclear structure.
Interproximal contact areas of primary molars based on OXIS classification – a two centre cross sectional study
Authors: Walia Tarun , Kirthiga M , Brigi Carel , Muthu M S , Odeh Ruba , Pakash Mathur Vijay , Rodrigues Steven 
Published: February 2021

Background: Contact areas in primary teeth are known to be broader, flatter and situated farther gingivally than permanent teeth. The purpose of this study was to assess the prevalence of OXIS contact areas between primary molars using die models of children from two different ethnic populations. The research question of the present study is “What is the prevalence OXIS contact areas of primary molars in the populations studied?”. Methods: A cross-sectional study was planned in a representative sample of 200 caries-free contact areas among children aged 3-6 years in two centers, Ajman and Puducherry. Data collection was performed from sectional or full-arch die stone models. The contacts were assessed according to OXIS classification by a single calibrated examiner at Center 2. Prevalence was expressed as numbers and percentages. The Chi-square test was applied to determine the association of OXIS contacts across genders and arches. Results: The prevalence of O, X, I, and S contacts were 19%, 22.5%, 53%, and 5.5% in Center 1 and 6%, 1.5%, 75.5%, and 17% in Center 2, respectively. Significant results (p=0.005) were obtained in Center 1 when inter-arch comparison was performed and between the centers with respect to type of contact. No significant difference was obtained with respect to gender and OXIS contact areas. Conclusions: The present study confirmed OXIS contacts in both the centers. The ‘O’ and ‘X’ types of contacts were observed more in Center 1, whereas ‘I’ and ‘S’ contacts were observed more in Center 2.
A Study on the Time Lag and Compensation of a Variable-Rate Fertilizer Applicator
Authors: Zhang Jinqin , Liu Gang , Huang Jiayun , Zhang Yaohui 
Published: February 2021

HighlightsA lag time detection system for variable-rate fertilization was developed.The lag time of a variable-rate fertilizer applicator was obtained and analyzed.A sigmoid equation was fitted to the data of rate change transition tests.The planar coordinates-based lag distance compensation method (LDCM) could reduce the lag distance effectively.Abstract. The location accuracy of fertilizer application is an essential aspect of the performance of variable-rate fertilizer applicators. The lag time of the fertilization system is an important cause of fertilizer rate transition lags. In order to obtain the lag time and make proper corrections, we developed a lag time detection system for a fluted roller-based variable-rate fertilizer applicator, taking into account the distance between the on-tractor GNSS antenna and the applicator furrow openers, and applied a planar coordinates-based lag distance compensation method (LDCM) to reduce the lag distance. To verify the performance of the LDCM, we conducted fertilization tests with and without LDCM at tractor forward speeds of 3.8, 5.5, and 8 km/h. First, the lag time detection sensors were installed on the fertilizer applicator, and the lag times were measured. Then, the corrected relative position coordinates of the fertilizer outlets were calculated according to the real-time speed and position data from the GNSS receiver. By implementing the control function of the applicator, the fertilization lags were corrected. A sigmoid equation was fitted to the rate change transition data. The results showed that for rate changes from 200 to 325 kg/ha, the delay distances were reduced from 1.10 to -0.84 m (at V = 3.8 km/h), from 1.97 to 0.09 m (at V = 5.5 km/h), and from 6.38 to 0.80 m (at V = 8 km/h). As a result, the LDCM can efficiently decrease lag distances of the variable-rate fertilizer applicator and meet the requirements of accurate fertilization in precision agriculture. Keywords: Fertilization lag, Lag distance compensation, Lag time, Variable-rate fertilization.
Integration of Auxiliary Sensor Data to ISOBUS for Agricultural Machinery Data Collection
Authors: Stoll Gabe P. , Luck Joe D. , Pitla Santosh K. , Rohrer Rodney A. 
Published: February 2021

HighlightsA novel data acquisition system was developed to synchronously log CANbus machine operating parameters with auxiliary sensor data converted and transmitted via the ISObus.A relationship between the number of added messages and frequency of transmission was evaluated.Knowledge of existing bus loads can lead to successful deployment of the data acquisition system for field data logging of both machine and implement data.Abstract. While standalone CAN data loggers offer a simplified, plug-and-play solution for agricultural machinery performance data collection, information that can be obtained from these devices are limited to data contained within interpretable messages broadcast over the machinery CAN bus. For machinery performance parameters that cannot be interpreted from CAN data, alternative methods are needed to record these variables. This application note discusses the development of a customized solution, named the Sensor CAN Gateway (or SCANGate), that integrates various components, including an Electronic Controller Unit (ECU), to allow auxiliary sensor data to be recorded by a CAN data logger through message transmission over a tractor’s ISOBUS. Testing with a modern tractor validated that the impact on bus load from added messages transmitted by the SCANGate could be determined based on the number of additional messages added per time interval. With knowledge of existing bus load requirements for an implement connected to a tractor’s ISOBUS, it can be determined whether the SCANGate can further enhance a standalone CAN data logger for a given application. Keywords: Agricultural machinery, CAN bus, Controller Area Network (CAN), Data acquisition, SAE J1939.
Silicon reconfigurable mode-selective modulation for on-chip mode-multiplexed photonic systems
Authors: Zhou Gangqiang , Guo Yuyao , Lu Liangjun , Chen Jianping , Zhou Linjie 
Published: February 2021

Recently, optical mode-division multiplexing has drawn a lot of attention due to its ability to increase the optical communication capacity in one physical channel with a single wavelength carrier. In this Letter, we demonstrate reconfigurable mode-selective modulation which is potentially useful for on-chip mode-multiplexed photonic systems. The device consists of two mode exchangers and one

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mode modulator. The mode exchanger is based on a Mach–Zehnder interferometer that performs mode exchange between

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modes. The

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mode modulator consists of a pair of

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multimode interferometers acting as a mode (de)multiplexer. It only selectively modulates the

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mode while bypassing the

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mode. 32 Gb/s on-off keying (OOK) modulation is successfully demonstrated for both input

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modes. This device can be used as a building block for on-chip multimode interconnect networks.
In-hospital mortality from severe COVID-19 in a tertiary care center in Mexico City; causes of death, risk factors and the impact of hospital saturation
Authors: Olivas-Martínez Antonio , Cárdenas-Fragoso José Luis , Jiménez José Víctor , Lozano-Cruz Oscar Arturo , Ortiz-Brizuela Edgar , Tovar-Méndez Víctor Hugo , Medrano-Borromeo Carla , Martínez-Valenzuela Alejandra , Román-Montes Carla Marina , Martínez-Guerra Bernardo , González-Lara María Fernanda , Hernandez-Gilsoul Thierry , Herrero Alfonso Gulias , Tamez-Flores Karla María , Ochoa-Hein Eric , Ponce-de-León Alfredo , Galindo-Fraga Arturo , Kershenobich-Stalnikowitz David , Sifuentes-Osornio José 
Published: February 2021

Background
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has remained in Latin America, Mexico has become the third country with the highest death rate worldwide. Data regarding in-hospital mortality and its risk factors, as well as the impact of hospital overcrowding in Latin America has not been thoroughly explored.

Methods and findings
In this prospective cohort study, we enrolled consecutive adult patients hospitalized with severe confirmed COVID-19 pneumonia at a SARS-CoV-2 referral center in Mexico City from February 26th, 2020, to June 5th, 2020. A total of 800 patients were admitted with confirmed diagnosis, mean age was 51.9 ± 13.9 years, 61% were males, 85% were either obese or overweight, 30% had hypertension and 26% type 2 diabetes. From those 800, 559 recovered (69.9%) and 241 died (30.1%). Among survivors, 101 (18%) received invasive mechanical ventilation (IMV) and 458 (82%) were managed outside the intensive care unit (ICU); mortality in the ICU was 49%. From the non-survivors, 45.6% (n = 110) did not receive full support due to lack of ICU bed availability. Within this subgroup the main cause of death was acute respiratory distress syndrome (ARDS) in 95% of the cases, whereas among the non-survivors who received full (n = 105) support the main cause of death was septic shock (45%) followed by ARDS (29%). The main risk factors associated with in-hospital death were male sex (RR 2.05, 95% CI 1.34–3.12), obesity (RR 1.62, 95% CI 1.14–2.32)—in particular morbid obesity (RR 3.38, 95%CI 1.63–7.00)—and oxygen saturation < 80% on admission (RR 4.8, 95%CI 3.26–7.31).

Conclusions
In this study we found similar in-hospital and ICU mortality, as well as risk factors for mortality, compared to previous reports. However, 45% of the patients who did not survive justified admission to ICU but did not receive IMV / ICU care due to the unavailability of ICU beds. Furthermore, mortality rate over time was mainly due to the availability of ICU beds, indirectly suggesting that overcrowding was one of the main factors that contributed to hospital mortality.
Risk factors for ≥high-grade anal intraepithelial lesions in MSM living with HIV and the response to topical and surgical treatments
Authors: Hidalgo-Tenorio Carmen , García-Martínez Carmen Maria , Pasquau Juan , Omar-Mohamed-Balgahata Mohamed , López-Ruz Miguel , López-Hidalgo Javier , Gil-Anguita Concepción 
Published: February 2021

Background
The objective of this study in MSM living with HIV was to determine the incidence of HSIL and ASCC, related factors, and the response to treatment.

Patients and methods
Data were gathered in 405 consecutive HIV-infected MSM (May 2010-December 2018) at baseline and annually on: sexual behavior, anal cytology, and HPV PCR and/or high-resolution anoscopy results. They could choose mucosectomy with electric scalpel (from May 2010) or self-administration of 5% imiquimod 3 times weekly for 16 weeks (from November 2013). A multivariate logistic regression model was developed for ≥HSIL-related factors using a step-wise approach to select variables, with a significance level of 0.05 for entry and 0.10 for exit, applying the Hosmer-Lemeshow test to assess the goodness of fit.

Results
The study included 405 patients with a mean age of 36.2 years; 56.7% had bachelor´s degree, and 52.8% were smokers. They had a mean of 1 (IQR 1–7) sexual partner in the previous 12 months, median time since HIV diagnosis of 2 years, and mean CD4 nadir of 367.9 cells/uL; 86.7% were receiving ART, the mean CD4 level was 689.6 cells/uL, mean CD4/CD8 ratio was 0.77, and 85.9% of patients were undetectable. Incidence rates were 30.86/1,000 patient-years for ≥high squamous intraepithelial lesion (HSIL) and 81.22/100,000 for anal squamous cell carcinoma (ASCC). The ≥HSIL incidence significantly decreased from 42.9% (9/21) in 2010 to 4.1% (10/254) in 2018 (p = 0.034). ≥HSIL risk factors were infection with HPV 11 (OR 3.81; 95%CI 1.76–8.24), HPV 16 (OR 2.69, 95%CI 1.22–5.99), HPV 18 (OR 2.73, 95%CI 1.01–7.36), HPV 53 (OR 2.97, 95%CI 1.002–8.79); HPV 61 (OR 11.88, 95%CI 3.67–38.53); HPV 68 (OR 2.44, CI 95% 1.03–5.8); low CD4 nadir (OR1.002; 95%CI 1–1.004) and history of AIDS (OR 2.373, CI 95% 1.009–5.577). Among HSIL-positive patients, the response rate was higher after imiquimod than after surgical excision (96.7% vs 73.3%, p = 0.009) and there were fewer re-treatments (2.7% vs 23.4%, p = 0.02) and adverse events (2.7% vs 100%, p = 0.046); none developed ASCC.

Conclusions
HSIL screening and treatment programs reduce the incidence of HSIL, which is related to chronic HPV infection and poor immunological status. Self-administration of 5% imiquimod as first-line treatment of HSIL is more effective than surgery in HIV+ MSM.