bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-Photon Microscopy for the Evaluation of Interstitial Fibrosis in Extended Criteria Donor Kidneys: A Proof-of-Concept Study
Authors: So Wei Zheng , Teo Rachel Zui Chih , Ooi Li Yin , Goh Benjamin Yen Seow , Lu Jirong , Vathsala Anantharaman , Thamboo Thomas Paulraj , Tiong Ho Yee 
Published: November 2021

Abstract
Introduction:This study evaluated the initial use of label-free second harmonic generation (SHG) imaging with two-photon excitation (2PE) auto-fluorescence for the quantification of collagen/fibrosis on pre-implantation biopsies of extended criteria donors (ECD).Methods:A total of 20 core ECD kidney transplant biopsy specimen tissues from 7 ECD donors, taken at the time of pre-implantation were retrieved, cut into 5-micron sections, mounted on slides and deparaffinized. The core needle biopsies were imaged with 2X and 20X objective using the commercially available laser-based Genesis® 200 (Histoindex Pte Ltd and Clinnovate Pte Ltd, Singapore). The entire core was selected as the Region of Interest (ROI). Corresponding clinical information from transplant donors were retrieved. Histopathological review was performed, and all biopsies had Interstitial Fibrosis (IF)/Tubular Atrophy (TA) scores > 0. Collagen parameters measured included quantification by the Collagen Area Ratio in Tissue (CART) and qualitative measurements by Collagen Reticulation Index (CRI).Results: 20 explant core biopsies were extracted from 10 donor kidney samples, of which originated from 7 donors. Table 1 depicts the baseline ECD characteristics of the donors. For the kidneys with multiple biopsies done, we obtained an average score of the collagen parameters across the different samples. Biopsies classified with > 85% KDPI score had significantly higher CAR and CART than biopsies with ≤ 85% KDPI score.Conclusion:MPM is an evolving technology that enables the quantification of the amount (CART) and quality (CRI) of collagen deposition in unstained explant biopsies of ECD kidneys. This initial evaluation found significant differences in both parameters between ECD kidneys with more or less than 85% KDPI scores.
Diverse recruitment to a taxonomically structured global atmospheric microbiota
Authors: Archer Stephen , Lee Kevin , Caruso Tancredi , Leung Marcus , Tong Xinzhao , Salter Susannah J. , Hinchliffe Graham , Maki Teruya , Santl-Temkiv Tina , Warren-Rhodes Kimberley , Gomez-Silva Benito , Hyde Kevin , Liu Celine , Alcamí Antonio , Al-Mailem Dina , Araya Jonathan , Cary Stephen , Cowan Don , Dempsey Jessica , Etchebehere Claudia , Gantsetseg Batdelger , Hartery Sean , Harvey Mike , Hayakawa Kazuichi , Hogg Ian , Inoue Mutsoe , Kansour Mayada , Lawrence Tim , Lee Charles , Leopold Matthius , McKay Christopher , Nagao Seiya , Poh Yan Hong , Ramond Jean-Baptiste , Rastrojo Alberto , Sekiguchi Toshio , Sim Joo Huang , Stahm William , Sun Henry , Tang Ning , Vandenbrink Bryan , Walther Craig , Lee Patrick , Pointing Stephen Brian 
Published: November 2021

Abstract
Atmospheric transport is critical to dispersal of microorganisms between habitats and this underpins resilience in terrestrial and marine ecosystems globally. A key unresolved question is whether microorganisms assemble to form a taxonomically distinct, geographically variable, and functionally adapted atmospheric microbiota. Here we characterised inter-continental patterns of microbial taxonomic and functional diversity in air within and above the atmospheric boundary layer and in underlying soils for 596 globally sourced samples. Bacterial and fungal assemblages in air were taxonomically structured and deviated significantly from purely stochastic assembly. Patterns differed with location and reflected underlying surface cover and environmental filtering. Source-tracking indicated a complex recruitment process involving local soils plus globally distributed inputs from drylands and the phyllosphere. Assemblages displayed stress-response and metabolic traits relevant to survival in air, and taxonomic and functional diversity were correlated with macroclimate and atmospheric variables. Our findings highlight complexity in the atmospheric microbiota that is key to understanding regional and global ecosystem connectivity.
Animal models of ovariectomy in rats demonstrate characteristics similar to early stage osteoarthritis
Authors: Casonato Natália Aparecida , de Araújo Camila Marques , Trevisan Mariane Santos , Lima Cristina Arrais , Vasilceac Fernando Augusto 
Published: November 2021

Abstract
Purpose

The aim of our study is to analyze the model of ovariectomy (OVX) in rats reproduced histological changes of osteoarthritis (OA).
Methods

For the development of the research, 12 Wistar rats were used, divided into 2 equal groups: Control Group - C (n = 6) and Osteoarthritis Group - OA (n = 6). After the 6-month experimental period, all rats were sacrificed and, subsequently, the entire knee joint complex was removed without disarticulation. For the histological evaluation of the tissue, the recommendations of the International Society for Research in OA (OARSI) were used. For data processing, each evaluation was statistically treated in both groups, comparing data from group C with the group OA. Results: Through the histological evaluation of OARSI, the evolution of OA in various tissues of the joint was evaluated. Although the OA group showed noticeable differences from group C, they were not as significant. Thus, only statistically significant favors were presented in the loss of the cartilaginous matrix (OA and C, p = 0.51), considering that the changes in the loss of ECM occurred only at the depth of 0% (superficial region), but at the depth of 50% (intermediate region) and 100% depth (deep region) did not exist.
Conclusion

Our study demonstrated that the OVX model is a good model to discuss OA, showing histological changes similar to those found in OA, the model demonstrated to have a progressive and slow characteristic since after the OARSI evaluations, prominent evidence was found in the initial manifestations of OA.
Comparison of Shiraz Trauma Transfusion Score (STTS) Potency With Common Scoring Systems for Trauma in Predicting the Short and Long Term Mortality of Injured Patients
Authors: Rahbari Sepideh Vosooghi , Hosseinpour Hamidreza , Karoobi Mohamadreza , Abolghasemi Hojat , Shahabinezhad Ali , Paydar Shahram , Sheida  
Published: November 2021

Abstract
Background and Objective: Trauma is the leading cause of death in people under 40 years of age worldwide. Various studies have been conducted focused on reducing the annual mortality rate due to trauma. One of the most important measures is reducing the time between the incident and the treatment set up, therefore the estimation of the severity of trauma and progressing to mortality before further evaluation is justified. In this study, we aim to compare different trauma scoring systems (such as GAP, MGAP, RTS, TRISS) with a relatively new model – Shiraz Trauma Transfusion Score (STTS) – and to describe the best qualities of these scoring systems for trauma patients in short (less than 24 hours) and long (more than 24 hours) term.Methods: In this cross-sectional study, data from hospitalized trauma patients in Rajaei hospital (center B) of Shiraz, Iran from May to November 2016 were collected and analyzed. Collected data consisted of age, sex, hospital admission duration, mechanism of trauma along with clinical data for calculating different trauma scoring systems, were recorded. Results: while RTS and STTS were the best predictors of mortality in trauma patients in the first 24 hours (sensitivity of 100.00%), GAP and MGAP were the best predictors of the patients’ survival (specificity of 93.83% and 92.59%). GAP and ISS were the best predictors of mortality in trauma patients for more than 24 hours (sensitivity of 82.02%). On the other hand, TRIS and RTS were the best predictors of patients' survival (specificity of 82.59% and 80.26%).Conclusions: Our study findings suggest that the utility and applicability of Shiraz Trauma Transfusion Score(STTS) in predicting mortality is not only comparable with other commonly used scoring methods but it may be of more value in shortterm mortality prediction.
Polyserositis revealing a signet ring cells gastric adenocarcinoma in a 27 years old male patient: A case report.
Authors: Alaoui Zahid Talibi , Youbi Salah Eddine , Ihbibane Fatima , Rachidi Hind , Hazmiri Fatima Ezzahra , Rais Hanane , Tassi Noura 
Published: November 2021

Abstract
Gastric cancer remains one of the most common and deadly cancers worldwide, especially among old males. It is rare in the younger population (< 30 years old). We describe the case of a 27 years old male patient, presenting to the emergency department, with exsudative polyserositis, initially misdiagnosed and treated as a multifocal tuberculosis with no clinical improvement, later revealing a metastatic signet ring cells gastric adenocarcinoma.
Efficacy of Clinically Used Anthelmintics Against Toxocariasis of Buffalo Calves in Bangladesh
Authors: Biswas Hiranmoy , Roy Babul Chandra , Dutta Pallab Kumar , Hasan Mohammad Manjurul , Begum Nurjahan , TALUKDER MD Hasanuzzaman 
Published: November 2021

Abstract
Toxocariasis is a paramount parasitic disease having over 50% prevalence among newborn buffalo calves in Bangladesh. The objective of this study was to compare the efficacy of clinically used anthelmintics and their subsequent effects on the hematological parameters and body weight in buffalo calves in commercial buffalo farms in coastal region. Thirty-two buffalo calves below 3 months of age with clinical Toxocara infection had been selected and treated with albendazole (ABZ), levamisole (LVM) and ivermectin (IVM). The EPG counts, hematological parameters and initial body weight of buffalo calves were recorded on the day of anthelmintic treatment (day 0). Fecal samples were collected on day 14 and 28 including hematological parameters and body weight were recorded on day 28 post-treatment. The efficacy (%) of anthelmintics were estimated by fecal egg count reduction test (FECRT). The parameters like Hb, PCV, ESR, TEC, TLC were analyzed from the blood samples. The FECRT revealed 96.83% efficacy for IVM followed by 94.23% and 85.84% for LVM and ABZ, respectively. Anthelmintic treated calves showed significant (p<0.01) increase in Hb, PCV, TEC and body weight, and decrease in ESR and TLC as a result of worm expulsion from buffalo calves after 28 days of post-treatment. Among the tested anthelmintics, IVM was found to be more effective against toxocariasis in buffalo calves. This is a novel new information on anthelmintics efficacy in buffalo calves in Bangladesh. Further studies are imperative to explore the factors responsible for high prevalence of this deadly nematode in buffalo calves.
Evaluation of the chemical composition of aquatic environments with leaf litter decomposition of Eucalyptus urophylla S.T. Blake (Myrtaceae) and their toxicity in Allium cepa L. (Amaryllidaceae)
Authors: Guerino Ritielly Maria Guimarães , Silva Junilson Augusto , Pires Débora de Jesus , Souza Rafael Aparecido Carvalho , Sousa Raquel Maria Ferreira , Oliveira Alberto , Morais Isa Lucia 
Published: November 2021

Abstract
Allelochemicals from eucalyptus released into the environment, mainly by leaves, can have a toxic effect on local biota, including in aquatic environments. Therefore, the present study evaluates the toxic activity of the water containing leaves of Eucalyptus urophylla S.T. Blake (Myrtaceae) in decomposition using Allium cepa L. (Amaryllidaceae) as a test organism. The toxicity and the cytotoxicity evaluation were performed using onion bulbs (A. cepa). The toxicity was assessed by inhibiting root growth. The cytotoxicity was evaluated by using a comparison of the mitotic index (MI) and the negative control. The extraction of eucalyptus metabolites from water of the decomposition tests and creek water was performed by solid-phase microextraction (SPME). The chemical characterization was done by gas chromatography coupled to mass spectrometry (GC-MS). There was inhibition of the root growth of A. cepa, indicating toxicity of the compounds released in the water during the decomposition. The cytotoxicity tests did not indicate a toxic effect. However, there were identified some mutations, cell death, and morphological changes in the roots. 26 compounds were identified on samples of water acquired from decomposition tests.Fenchone, 2-ethyl-1-hexanol, cis-dihydrocarvone, and trans-dihydrocarvone were identified in all samples. The results highlight the importance of studies and monitoring of aquatic environments near eucalyptus.
Highly sensitive HBsAg, anti-HBc and anti HBsAg titres in Early Diagnosis of HBV Reactivation in Anti-HBc-Positive onco-haematological Patients.
Authors: Cerva Carlotta , Salpini Romina , Alkhatib Mohammad , Malagnino Vincenzo , Piermatteo Lorenzo , Battisti Arianna , Bertoli Ada , Gersch Jeff , Holzmayer Vera , Kuhns Mary , Cloherty Gavin , Ferrari Ludovica , Laura Campogiani , Teti Elisabetta , Cantonetti Maria , Arcese William , Ceccherini-Silberstein Francesca , Perno Carlo-Federico , Andreoni Massimo , Svicher Valentina , Sarmati Loredana 
Published: November 2021

Abstract
The role of novel HBV markers in predicting Hepatitis B virus reactivation (HBV-R) in HBsAg-negative/anti-HBc-positive oncohaematological patients was examined. One hundred and seven HBsAg-negative/anti-HBc-positive oncohaematological patients, receiving anti-HBV prophylaxis for > 18 months were included. At baseline, all patients had undetectable HBV DNA, and 67.3% were anti-HBs positive. HBV-R occurred in 17 (15.9%) patients: 6 during and 11 after the prophylaxis period. At HBV-R, the median (IQR) HBV-DNA was 44 (27–40509) IU/ml, and the alanine aminotransferase upper limit of normal (ULN) was 44% (median [IQR]: 81[49–541] U/L). An anti-HBc>3 cut-off index (COI) plus anti-HBs persistently/declining to <50 mIU/ml was predictive for HBV-R (OR [95% CI]: 9.1 [2.7–30.2]; 63% of patients with vs. 15% without this combination experienced HBV-R, P<0.001). The detection of highly sensitive (HS) HBsAg and/or HBV-DNA confirmed at > 2 time points, also predicts HBV-R (OR [95% CI ]: 13.8 [3.6–52.6]; 50% of positive vs. 7% of negative patients to these markers experienced HBV-R, P=0.001).HS-HBs and anti-HBc titration proved useful early markers of HBV-R.The use of these markers demonstrated that HBV-R frequently occurs in oncohaematological patients with signs of resolved HBV infection, raising issues of proper HBV-R monitoring.
Hacia el envejecimiento activo y saludable: una experiencia de cine-debate en jubilados
Authors: Aguilera-Velasco María de los Ángeles , Acosta-Fernández Martín , Pozos-Radillo Blanca Elizabeth , Franco-Chávez Sergio Adalberto 
Published: November 2021

Introducción: Ante el potencial del cine para formar hacia el envejecimiento activo y saludable, sumado a las amplias recomendaciones fílmicas existentes donde los protagonistas son precisamente adultos mayores en proceso de jubilación y vejez, se trazaron los objetivos de analizar la experiencia de cine-debate sobre el envejecimiento saludable y el envejecimiento activo y sus determinantes, en personas jubiladas agremiadas, y determinar las aportaciones de cada filme durante el proceso de visualización.
Método: Estudio con enfoque cualitativo y diseño preexperimental descriptivo transversal exploratorio, a través de una intervención socioeducativa de cine-debate, durante un periodo de siete meses previos a la pandemia por Covid-19. Participaron 19 personas. Para la recolección de los datos se utilizó la técnica de grupo focal grabado en audio. Los datos obtenidos se analizaron bajo el fundamento de la fenomenología. Las sesiones de cine-debate se realizaron en la casa de día para jubilados. El programa de intervención se conformó con 25 películas.
Resultados: Dentro del envejecimiento saludable, los participantes analizaron el fomento y el mantenimiento de su capacidad funcional y su interacción consigo mismos, con su pareja, con sus hijos, con la sociedad y con sus entornos. Sobre el envejecimiento activo, lograron visualizar sus oportunidades de salud, de participación y seguridad. Expresaron su sabiduría de vida, para enfrentar su jubilación y para vivir con amor. Debatieron sobre sus lazos familiares y comunitarios, así como el empoderamiento, la independencia, la autonomía, la dignidad y el autodesarrollo. Identificaron los determinantes físicos, sociales, económicos, personales (psicológicos y biológicos) y del estilo de vida que influyen en el envejecimiento activo. Se hallaron también las aportaciones particulares de todas las películas para cada parte del proceso educativo.
Conclusión: Todos los filmes del ciclo de cine-debate fueron indispensables para lograr la experiencia compartida sobre el envejecimiento saludable y el envejecimiento activo y sus determinantes. Se propone hacer nuevas investigaciones con películas cuyos protagonistas sean igualmente que en este estudio adultos mayores, pero que los filmes sean de reciente producción.
Studying the effect of design parameters on riverbank filtration performance for drinking water supply in Egypt: a case study
Authors: Mamdouh Heba , Abdel Wahaab Rifaat , Khalifa Abdelkawi , Elalfy Ezzat 
Published: November 2021

Abstract
Riverbank filtration (RBF) is an affordable technique to provide drinking water with adequate quality. The ultimate objective of this study is to facilitate the transferability and application of this sustainable technique in Egypt. In this work, a numerical model was constructed using Groundwater Modeling System (GMS) to study the effect of four design parameters on the RBF performance parameters (i.e., river filtrate portion and travel time) with the aid of MODPATH and ZONEBUDGET. The design parameters were: (1) the pumping rates of the RBF wells, (2) number of operating wells, (3) distance between wells and the river, and (4) the spacing between wells. This study was focused on the hydraulic aspects of the technique. The results demonstrated that: (1) the river filtrate portion exceeds 75% regardless of the design conditions, and (2) the hydraulic performance of the RBF technique is highly controlled by the production capacity of the wells and their positions relative to the surface water systems; the spacing between wells has a minimum effect. Two equations were developed to estimate the river filtrate portion and minimum travel time as functions of pumping rate and distance between the pumping well and the river.
Abstraction of Minahasan folklore in food tradition
Authors: Pamantung Rina Palisuan , Mantau Mercy , Sahetapy Johan F. , Manangkot Verra E. 
Published: November 2021

The main purpose of folklore is to convey present useful information and everyday life lessons in an easy way for the common people to understand. "The transmission process of folklore is still largely undocumented." Minahasan people still believe that Folklore become a philosophy in life. That philosophy never changes although time is changed until era 0.5. This research is entitled “Abstraction of Minahasan Folklore in Food”. The problems are the form and meaning of abstraction of Minahasan folklore in food. The method used is a qualitative method through an ethnography at the level of descriptive synchronic linguistics. An ethnographic approach was applied in data collection so that the form and meaning of Minahasan traditional food are in line with the concept of people’s of Minahasa. This research uses the Gastronomy linguistics approach and purposive sampling. The theory of Syntax by Givon (1994) and theory of meaning by Leech (1981) are used to analyze the data. The results showed that Minahasan Folklore consist of several titles are as follow: I royor si koki’ ,Toar Lumimuut, Si Naa en Jaa,Tumideng (Tumileng), Lingkambene (padi yang melambai), Wuwun Sewe, Tjarema, Sisil an dumoro’ in tjinamualian in tama i Maesa tanu in and Panagian.
RESEARCH FOR A COMPREHENSIVE AND ACTIVE PLANNING METHOD IN AN INDUSTRIAL-RESIDENTIAL MIXED AREAFOCUSED ON OTA CREATIVE TOWN VISION IN OTA WARD, TOKYO
Authors: Nohara Taku , Okamura Yu , Kawabara Susuma 
Published: November 2021

Recently in Japan, the urban environment in industrial cities has been changing due to changes in the industrial, economical, and social structure. Ota Ward in Tokyo has small industrial districts, some of which are mixed-use areas. In the 1980â€™s there were 9,000 factories, but currently that number has been decreasing and although the factories still possess sophisticated skill-sets, their working environment is inadequate as it issurrounded by too many residential dwellings. In addition, it is too difficult to pass technical knowledge onto succeeding generations. In this research, we focus on a comprehensive and active planning method, specifically, on how to integrate manufacturing, community development, and city promotion so as to solve this kind of problem in mixed residential-industrial cities. Firstly, we investigated the local resources, which consisted of a small industrial network of a â€œmixed-usedâ€ status in Ota Ward that had access to an international airport. Secondly, we created a visionÂ encompassing these three points: (1) Stock (property) management, (2) Supporting Creative Industry, and (3) City Promotion via Industrial Tourism.Currently two projects are in progress: One is the industrial tourism event, â€œOta Open Factoryâ€, which is a one day program where many restricted access small factories are opened to the public in the mixed-used industrial area; and the other is â€œCreative Town Lab. Tamagawaâ€, which is an active community space (usable for education, workshops, meetings, exhibitions, events) that has been converted from a vacant factory.With these projects, we are trying to realize the areaâ€™s potential through activities, recreating a more creative and active city culture and identity.