bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutron optical test of completeness of quantum root-mean-square errors
Authors: Sponar Stephan , Danner Armin , Ozawa Masanao , Hasegawa Yuji 
Published: June 2021

AbstractWhile in classical mechanics the mean error of a measurement is solely caused by the measuring process (or device), in quantum mechanics the operator-based nature of quantum measurements has to be considered in the error measure as well. One of the major problems in quantum physics has been to generalize the classical root-mean-square error to quantum measurements to obtain an error measure satisfying both soundness (to vanish for any accurate measurements) and completeness (to vanish only for accurate measurements). A noise-operator-based error measure has been commonly used for this purpose, but it has turned out incomplete. Recently, Ozawa proposed an improved definition for a noise-operator-based error measure to be both sound and complete. Here, we present a neutron optical demonstration for the completeness of the improved error measure for both projective (or sharp) as well as generalized (or unsharp) measurements.
Chemical profiling of DNA G-quadruplex-interacting proteins in live cells
Authors: Zhang Xiaoyun , Spiegel Jochen , Martínez Cuesta Sergio , Adhikari Santosh , Balasubramanian Shankar 
Published: June 2021

AbstractDNA–protein interactions regulate critical biological processes. Identifying proteins that bind to specific, functional genomic loci is essential to understand the underlying regulatory mechanisms on a molecular level. Here we describe a co-binding-mediated protein profiling (CMPP) strategy to investigate the interactome of DNA G-quadruplexes (G4s) in native chromatin. CMPP involves cell-permeable, functionalized G4-ligand probes that bind endogenous G4s and subsequently crosslink to co-binding G4-interacting proteins in situ. We first showed the robustness of CMPP by proximity labelling of a G4 binding protein in vitro. Employing this approach in live cells, we then identified hundreds of putative G4-interacting proteins from various functional classes. Next, we confirmed a high G4-binding affinity and selectivity for several newly discovered G4 interactors in vitro, and we validated direct G4 interactions for a functionally important candidate in cellular chromatin using an independent approach. Our studies provide a chemical strategy to map protein interactions of specific nucleic acid features in living cells.
High content genome-wide siRNA screen to investigate the coordination of cell size and RNA production
Authors: Müller Micha , Avar Merve , Heinzer Daniel , Emmenegger Marc , Aguzzi Adriano , Pelkmans Lucas , Berry Scott 
Published: June 2021

AbstractCoordination of RNA abundance and production rate with cell size has been observed in diverse organisms and cell populations. However, how cells achieve such ‘scaling’ of transcription with size is unknown. Here we describe a genome-wide siRNA screen to identify regulators of global RNA production rates in HeLa cells. We quantify the single-cell RNA production rate using metabolic pulse-labelling of RNA and subsequent high-content imaging. Our quantitative, single-cell measurements of DNA, nascent RNA, proliferating cell nuclear antigen (PCNA), and total protein, as well as cell morphology and population-context, capture a detailed cellular phenotype. This allows us to account for changes in cell size and cell-cycle distribution (G1/S/G2) in perturbation conditions, which indirectly affect global RNA production. We also take advantage of the subcellular information to distinguish between nascent RNA localised in the nucleolus and nucleoplasm, to approximate ribosomal and non-ribosomal RNA contributions to perturbation phenotypes. Perturbations uncovered through this screen provide a resource for exploring the mechanisms of regulation of global RNA metabolism and its coordination with cellular states.
A dataset of daily ambulatory psychological and physiological recording for emotion research
Authors: Shui Xinyu , Zhang Mi , Li Zhuoran , Hu Xin , Wang Fei , Zhang Dan 
Published: June 2021

AbstractTo better understand the psychological and physiological basis of human emotion, increasing interest has been drawn towards ambulatory recordings of emotion-related data beyond the laboratories. By employing smartphones-based ambulatory assessment and wrist-worn physiological recording devices, the Daily Ambulatory Psychological and Physiological recording for Emotion Research (DAPPER) dataset provides momentary self-reports and physiological data of people’s emotional experiences in their daily life. The dataset consists of ambulatory psychological recordings from 142 participants and physiological recordings from 88 of them over five days. Both the experience sampling method (ESM) and the day reconstruction method (DRM) were employed to have a comprehensive description of the participants’ daily emotional experiences. Heart rate, galvanic skin response, and three-axis acceleration were recorded during the day time. By including multiple types of physiological and self-report data at a scale of five days with 100+ participants, the present dataset is expected to promote emotion researches in real-life, daily settings.
Acquisition of novel ball-related skills associated with sports experience
Authors: Sekiguchi Hirofumi , Yamanaka Kentaro , Takeuchi Shigeki , Futatsubashi Genki , Kadota Hiroshi , Miyazaki Makoto , Nakazawa Kimitaka 
Published: June 2021

AbstractSome individuals can quickly acquire novel motor skills, while others take longer. This study aimed to investigate the relationships between neurophysiological state, sports experience, and novel ball-related skill acquisition. We enrolled 28 healthy collegiate participants. The participants’ neurophysiological data (input–output curve of the corticospinal tract) were recorded through transcranial magnetic stimulation. Subsequently, the participants performed a novel motor task (unilateral two-ball juggling) on a different day, after which they reported their previous sports experience (types and years). We found that individuals with more years of experience in ball sports showed faster acquisition of novel ball-related skills. Further, this result was not limited to any single ball sport. Therefore, the acquisition of novel ball-related skills is associated with familiarity with a ball’s nature. Furthermore, gain of the corticospinal tract was negatively and positively correlated with the years of experience in primary ball and non-ball sports (implemented for the longest time in individuals), respectively. These results could be associated with the extent of proficiency in their primary sport. The chosen type of sports (e.g., ball or non-ball) could critically influence the future acquisition of novel motor skills. This study provides important insights regarding how to approach sports and physical activities.
Electrocatalytic performance of NiNH2BDC MOF based composites with rGO for methanol oxidation reaction
Authors: Yaqoob Lubna , Noor Tayyaba , Iqbal Naseem , Nasir Habib , Mumtaz Asad 
Published: June 2021

AbstractPresent work comprehensively investigated the electrochemical response of Nickel-2 Aminoterephthalic acid Metal–Organic Framework (NiNH2BDC) and its reduced graphitic carbon (rGO) based hybrids for methanol (CH3OH) oxidation reaction (MOR) in an alkaline environment. In a thorough analysis of a solvothermally synthesized Metal–Organic Frameworks (MOFs) and its reduced graphitic carbon-based hybrids, functional groups detection was performed by FTIR, the morphological study by SEM, crystal structure analysis via XRD, and elemental analysis through XPS while electrochemical testing was accomplished by Chronoamperometry (CA), Cyclic Voltametric method (CV), Electrochemically Active Surface Area (EASA), Tafel slope (b), Electron Impedance Spectroscopy (EIS), Mass Activity, and roughness factor. Among all the fabricated composites, NiNH2BDC MOF/5 wt% rGO hybrid by possessing an auspicious current density (j) of 267.7 mA/cm2at 0.699 V (vs Hg/HgO), a Tafel slope value of 60.8 mV dec−1, EASA value of 15.7 cm2, and by exhibiting resistance of 13.26 Ω in a 3 M CH3OH/1 M NaOH solution displays grander electrocatalytic activity as compared to state-of-the-art platinum-based electrocatalysts.
Association between physical exercise and stroke recurrence among first-ever ischemic stroke survivors
Authors: Hou Lisha , Li Mier , Wang Ju , Li Yawen , Zheng Qianwen , Zhang Lu , Yao Qiang , Zhang Jing , Dong Shuju , Zhou Muke , Zhu Cairong 
Published: June 2021

AbstractThe relationship between exercise and stroke recurrence is controversial. This study was designed to test whether an association exists between exercise and ischemic stroke recurrence in first-ever ischemic stroke survivors. Data were collected from January 2010 to June 2016. Baseline information was obtained during face-to-face interviews, and follow-up phone interviews were conducted every 3 months. Exercise type, frequency, intensity, and duration were recorded. Discrete-time survival analysis was used to determine the relationship between exercise and stroke recurrence. 760 first-ever ischemic stroke survivors who were able to exercise were enrolled. After adjusting for covariates, patients who exercised 3.5–7 h per week and more than 7 h per week had a lower relapse risk than patients who did not exercise (3.5–7: OR 0.415; > 7: OR 0.356). Moreover, if the fluctuation of exercise duration was over 4 h, the patients had a higher risk of stroke recurrence than those with variability of less than 2 h (OR 2.153, P = 0.013). Stroke survivors who engage in long-term regular mild exercise (more than 5 sessions per week and lasting on average 40 min per session) have a lower recurrence rate. Irregular exercise increases the risk of stroke recurrence.
Exploring the chemical space of protein–protein interaction inhibitors through machine learning
Authors: Choi Jiwon , Yun Jun Seop , Song Hyeeun , Kim Nam Hee , Kim Hyun Sil , Yook Jong In 
Published: June 2021

AbstractAlthough protein–protein interactions (PPIs) have emerged as the basis of potential new therapeutic approaches, targeting intracellular PPIs with small molecule inhibitors is conventionally considered highly challenging. Driven by increasing research efforts, success rates have increased significantly in recent years. In this study, we analyze the physicochemical properties of 9351 non-redundant inhibitors present in the iPPI-DB and TIMBAL databases to define a computational model for active compounds acting against PPI targets. Principle component analysis (PCA) and k-means clustering were used to identify plausible PPI targets in regions of interest in the active group in the chemical space between active and inactive iPPI compounds. Notably, the uniquely defined active group exhibited distinct differences in activity compared with other active compounds. These results demonstrate that active compounds with regions of interest in the chemical space may be expected to provide insights into potential PPI inhibitors for particular protein targets.
Prospective evaluation of interrater agreement between EEG technologists and neurophysiologists
Authors: Beuchat Isabelle , Alloussi Senubia , Reif Philipp S. , Sterlepper Nora , Rosenow Felix , Strzelczyk Adam 
Published: June 2021

AbstractWe aim to prospectively investigate, in a large and heterogeneous population, the electroencephalogram (EEG)-reading performances of EEG technologists. A total of 8 EEG technologists and 5 certified neurophysiologists independently analyzed 20-min EEG recordings. Interrater agreement (IRA) for predefined EEG pattern identification between EEG technologists and neurophysiologits was assessed using percentage of agreement (PA) and Gwet-AC1. Among 1528 EEG recordings, the PA [95% confidence interval] and interrater agreement (IRA, AC1) values were as follows: status epilepticus (SE) and seizures, 97% [96–98%], AC1 kappa = 0.97; interictal epileptiform discharges, 78% [76–80%], AC1 = 0.63; and conclusion dichotomized as “normal” versus “pathological”, 83.6% [82–86%], AC1 = 0.71. EEG technologists identified SE and seizures with 99% [98–99%] negative predictive value, whereas the positive predictive values (PPVs) were 48% [34–62%] and 35% [20–53%], respectively. The PPV for normal EEGs was 72% [68–76%]. SE and seizure detection were impaired in poorly cooperating patients (SE and seizures; p < 0.001), intubated and older patients (SE; p < 0.001), and confirmed epilepsy patients (seizures; p = 0.004). EEG technologists identified ictal features with few false negatives but high false positives, and identified normal EEGs with good PPV. The absence of ictal features reported by EEG technologists can be reassuring; however, EEG traces should be reviewed by neurophysiologists before taking action.
Genetic variability assessment of 127 Triticum turgidum L. accessions for mycorrhizal susceptibility-related traits detection
Authors: Ganugi Paola , Masoni Alberto , Sbrana Cristiana , Dell’Acqua Matteo , Pietramellara Giacomo , Benedettelli Stefano , Avio Luciano 
Published: June 2021

AbstractPositive effects of arbuscular mycorrhizal fungi (AMF)—wheat plant symbiosis have been well discussed by research, while the actual role of the single wheat genotype in establishing this type of association is still poorly investigated. In this work, the genetic diversity of Triticum turgidum wheats was exploited to detect roots susceptibility to AMF and to identify genetic markers in linkage with chromosome regions involved in this symbiosis. A tetraploid wheat collection of 127 accessions was genotyped using 35K single-nucleotide polymorphism (SNP) array and inoculated with the AMF species Funneliformis mosseae (F. mosseae) and Rhizoglomus irregulare (R. irregulare), and a genome‐wide association study (GWAS) was conducted. Six clusters of genetically related accessions were identified, showing a different mycorrhizal colonization among them. GWAS revealed four significant quantitative trait nucleotides (QTNs) involved in mycorrhizal symbiosis, located on chromosomes 1A, 2A, 2B and 6A. The results of this work enrich future breeding activities aimed at developing new grains on the basis of genetic diversity on low or high susceptibility to mycorrhization, and, possibly, maximizing the symbiotic effects.
Design and synthesis of a novel nanocomposite based on magnetic dopamine nanoparticles for purification of α-amylase from the bovine milk
Authors: Eivazzadeh-Keihan Reza , Dogari Haniyeh , Ahmadpour Farnoush , Aliabadi Hooman Aghamirza Moghim , Radinekiyan Fateme , Maleki Ali , Fard Leyla Saei , Tahmasebi Behnam , Mojdehi Maryam Faraj Pour , Mahdavi Mohammad 
Published: June 2021

AbstractIn this paper, a novel nanocomposite based on magnetic nanoparticles decorated by dopamine were reported. Three modified magnetic nanocomposites by dopamine were offered with different type of linkers. The mentioned magnetic nanocomposites were applied to separate α-amylase protein from fresh bovine milk. All of the magnetic nanocomposites were characterized and investigated by using Fourier-transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, field-emission scanning microscope, X-ray diffraction pattern, and vibrating-sample magnetometer analyses. To investigate the purifying application, sodium dodecyl sulfate polyacrylamide gel electrophoresis, one-dimensional isoelectric focusing gel electrophoresis, and alpha-amylase activity assay were employed. With paying attention to factors such as yield of purification and concentration of separated protein by each of magnetic nanocomposite, it could be concluded that the length of linkers played an important role in α-amylase protein separation. According to the results, the best separation and purification of α-amylase protein with 49.83% recovery and 40.11-fold purification efficiency was related to longest length linker, 1,4-butanediol diglycidyl ether, because of considerable conjugation with nanocomposite. Also, docking calculation has shown that the binding energy is − 1.697 kcal/mol and ΔG = − 6.844 kcal/mol which result that the interaction process between dopamine and α-amylase protein is spontaneous.
Efficacy and safety of the application of extensive ablation in patients with atrioventricular re-entrant tachycardia (a retrospective study)
Authors: Xu Guangze , Chen Zhikui , Lin Haiyan 
Published: June 2021

AbstractRadiofrequency catheter ablation (RFCA) has become the standard effective therapy for supraventricular tachycardia, but the reported success rates of ablation have differed across a large number of single-center studies. The main reason for tachycardia recurrence is accessory pathway (Ap)-mediated tachycardia, and the use of the RFCA strategy may be related to recurrence. This study compared the efficacy and safety of two different RFCA strategies for Ap-mediated tachycardia. We compared patients (group M) who underwent RFCA at multiple sites to patients (group S) who underwent RFCA at a single site during the index procedure for Ap-mediated tachycardia. The efficacy and safety were assessed in the two groups. Follow-up was conducted, and the main complications and the incidence of recurrence after RFCA procedures were recorded. Eight hundred eighty-two patients with 898 Aps were enrolled in group S, and 830 patients with 843 Aps were enrolled in group M. The cumulative number of recurrences (rates) in group M and group S at the 1st, 3rd, 6th, 12th, and 24th months after ablation were 4 (0.5%) and 17 (1.9%), p < 0.05; 5 (0.6%) and 27 (3.0%), p < 0.05; 6 (0.7%) and 34 (3.8%), p < 0.05; 6 (0.7%) and 43 (4.8%), p < 0.05; and 7 (0.8%) and 45 (5.0%), p < 0.05, respectively. Complications of chest pain, overactive vasovagal reaction, steam pop, and angina pectoris were rare in both groups. One patient in group M suffered from myocardial infarction before extensive ablation. No valve damage, cardiac tamponade, or other serious adverse events occurred in either group. The extensive ablation strategy reduced the recurrence rate and the need for subsequent ablation of the Ap without increasing the risk of complications.
Validating the CHARMM36m protein force field with LJ-PME reveals altered hydrogen bonding dynamics under elevated pressures
Authors: Xu You , Huang Jing 
Published: June 2021

AbstractThe pressure-temperature phase diagram is important to our understanding of the physics of biomolecules. Compared to studies on temperature effects, studies of the pressure dependence of protein dynamic are rather limited. Molecular dynamics (MD) simulations with fine-tuned force fields (FFs) offer a powerful tool to explore the influence of thermodynamic conditions on proteins. Here we evaluate the transferability of the CHARMM36m (C36m) protein force field at varied pressures compared with NMR data using ubiquitin as a model protein. The pressure dependences of J couplings for hydrogen bonds and order parameters for internal motion are in good agreement with experiment. We demonstrate that the C36m FF combined with the Lennard-Jones particle-mesh Ewald (LJ-PME) method is suitable for simulations in a wide range of temperature and pressure. As the ubiquitin remains stable up to 2500 bar, we identify the mobility and stability of different hydrogen bonds in response to pressure. Based on those results, C36m is expected to be applied to more proteins in the future to further investigate protein dynamics under elevated pressures.
The effects of the chemical composition on the structural, thermodynamic, and mechanical properties of all-inorganic halide perovskites
Authors: Sánchez-Palencia Pablo , García Gregorio , Wahnón Perla , Palacios Pablo 
Published: June 2021

A systematicab-initiostudy of all-inorganic perovskites with formula CsPb1−bSnb(I1−xBrx)3 has been performed, elucidating the connection of that composition with their structural, thermodynamics and mechanical properties.
Survival from upper gastrointestinal cancer in New Zealand: The effect of distance from a major hospital, socio‐economic status, ethnicity, age and gender
Authors: Gill Andrew J. , Martin Iain G. 
Published: June 2021

BackgroundUpper gastrointestinal cancer is the third most common cause of cancer mortality in New Zealand. Prognosis of these and other cancers is possibly affected by the accessibility of hospitals. Several studies have revealed a reduction in survival that correlates with increasing distance from a major cancer centre. The aim of the present study was to analyse any correlation between survival from upper gastrointestinal cancer and distance from a major centre, socio‐economic status, gender, age and ethnicity.MethodsDetails of all 3351 patients diagnosed with cancers of the oesophagus, stomach, pancreas, liver and biliary tract between 1 January 1995 and 31 December 1997 were retrieved from the New Zealand cancer registry. The effect of age, gender, ethnicity, socio‐economic status and distance from a major centre was analysed using univariate and multivariate regression analysis to identify any associations with survival.ConclusionsIncreasing age and Maori descent were the only consistent indicators of poorer survival in this study. The relationship between distance and survival was shown to be complex and in this study deprivation had no effect on the prognosis of upper gastrointestinal cancer.
eROSITA X-ray scan of theηChamaeleontis cluster
Authors: Robrade J. , Czesla S. , Freund S. , Schmitt J. H. M. M. , Schneider P. C. 
Published: June 2021

Context.The nearby young open clusterηChamaeleontis has been observed by eROSITA/SRG during its CalPV phase for 150 ks. The extended ROentgen Survey with an Imaging Telescope Array (eROSITA) data were taken in the field-scan mode, an observing mode of Spectrum-Roentgen-Gamma (SRG) that follows a rectangular grid-like pattern, here covering a 5 × 5 deg field with an exposure depth of about 5 ks.Aims.TheηCha cluster with an age of about 8 Myr is a key target for investigating the evolution of young stars, and we aim to study the known members in X-rays. Additionally, we search for potential new members of the anticipated dispersed low-mass cluster population in a sensitive wide-field X-ray observation.Methods.Using eROSITA X-ray data, we studied theηCha region. Detected sources were identified by cross-matching X-ray sources withGalaand 2MASS, and young stars were identified by their X-ray activity, the position in the color-magnitude diagram, and by their astrometric and kinematic properties. X-ray-luminosities, light curves, and spectra of cluster members were obtained and compared with previous X-ray data. Literature results of other member searches were used to verify our new member candidates in the observed field.Results.We determine X-ray properties of virtually all knownηCha members and identify five additional stellar systems that show basically identical characteristics, but they are more dispersed. Four of them were previously proposed as potential members; this status is supported by our X-ray study. Based on their spatial distribution, further members are expected beyond the sky region we surveyed. The identified stellar systems very likely belong to the ejected halo population, which brings the total number ofηCha cluster members to at least 23.Conclusions.Sensitive X-ray surveys are best suited to identifying active stars, and the combination of the ongoing eROSITA all-sky survey withGalameasurements provides an unprecedented opportunity to study the nearby, young stellar population.