bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green discipline and involvement’s impact on green employee creativity and Innovation: mediating role of green empowerment in Yemeni public universities
Authors: Shamsan Riyadh , Farea Mazen Mohammed 
Published: February 2025

PurposeThis study aims to investigate the mediating role of green employee empowerment (GEE) in the relationship between green discipline management and green involvement with green employee creativity and innovation (GECI) within public universities in Yemen. The research explores how the components of green human resource management (GHRM) influence green creativity and innovation through employee empowerment.Design/methodology/approachThe study utilizes both primary and secondary data. The primary data were gathered through structured questionnaires distributed to 363 employees in public universities in Yemen. The data were analyzed using structural equation modeling (SEM) with AMOS to assess the relationships among the variables.FindingsThe findings reveal that green discipline management and green involvement have a significantly positive impact on GEE and GECI. Furthermore, GEE partially mediates this relationship, which demonstrates that empowered employees are more likely to contribute to environmentally sustainable creativity and innovation.Originality/valueThis research helps better understand how GHRM practices, such as discipline management and involvement, can promote green creativity and innovation through employee empowerment. By examining the mediating role of GEE, the study provides valuable insights for academic institutions and organizational leaders seeking to integrate sustainability into human resource (HR) practices and policies.
Research on intra-layer partition adaptive slicing algorithm for fused deposition modeling
Authors: Shen Sijia , He Ketai , Yu Biqiang , Zhai Chenlong , Ji Tianyan 
Published: February 2025

Purpose
This paper proposes a new intra-layer partition adaptive slicing algorithm for FDM 3D printing, aiming to further improve forming efficiency based on the adaptive slicing algorithm while preserving the surface finish quality of the formed model.

Design/methodology/approach
This method initially applies a large layer thickness for primary slicing, then refines layer thickness in layer height ranges with significant cross-sectional contour changes. Refined layers are partitioned: the internal region uses the large layer thickness for efficiency, while the external region uses a smaller layer thickness for surface quality. A thickness ratio and transition zone between regions prevent overlaps and gaps in printing paths.

Findings
The experimental results show that, compared to traditional adaptive slicing algorithms, the intra-layer partition adaptive slicing algorithm can effectively improve forming efficiency for most models while ensuring the model’s surface finish, with minimal impact on the bonding strength of the model.

Originality/value
The intra-layer partition adaptive slicing algorithm is a novel algorithm improved upon the traditional adaptive slicing algorithm, enabling models to achieve higher printing efficiency while maintaining the surface finish provided by the conventional adaptive slicing algorithm. This algorithm is of significant importance to vendors and individual users who provide printing services for large-sized fused deposition modeling models, as it can greatly enhance their production efficiency.
Interactions between the promotion of prosocial and pro-self values, and entrepreneurial social identities in shaping value creation aims and passion
Authors: Vörös Zsófia , Kehl Dániel 
Published: February 2025

Purpose
Due to the different logic of economic or entrepreneurial, and social value creation, hybrid entrepreneurs (entrepreneurs whose organization is committed to create both entrepreneurial and social values) often make compromises at the expense of economic rationality or social value creation. The purpose of this paper is to examine how entrepreneurial social identities interact with the promotion of pro-self and prosocial values to influence hybrid entrepreneurs’ choice of predominant values to create and their entrepreneurial passion.

Design/methodology/approach
To answer the research questions, we designed an experiment using an established priming protocol to temporarily increase the saliency of prosocial and pro-self cultural values. In the experiment, students (n = 203) with no entrepreneurial experience chose a hybrid venture to join its founding team in three conditions: neutral (control group, n = 72), social priming (n = 65) and commercial priming (n = 66).

Findings
The results indicate that behavioral choices on the type of the predominant value to create are linked to entrepreneurial social identities. The temporary increase in the saliency of prosocial or pro-self cultural values does not affect the predominant value to create through hybrid entrepreneurial venturing, but influences how passionately entrepreneurs act.

Research limitations/implications
The results are based on the behavior of university students who did not risk losing income because of their behavioral choice and could not opt out of entrepreneurship. Even if university students resemble the population of interest, field studies could contribute to the examination of the research questions of this explorative study and corroborate the results. Future studies should examine if, when and how the promotion of prosocial and pro-self cultural values may change entrepreneurs’ social identities and primary value creation aims.

Originality/value
The entrepreneurship literature rarely takes into consideration that the effects of cultural values might fluctuate along entrepreneurs’ personality traits and identities. This paper examines how entrepreneurial social identities and the temporary promotion of prosocial, and pro-self values interact to influence value creation choices during hybrid entrepreneurial venturing.
Molecular phylogeny reveals three new water mite species of the genus Teutonia Koenike, 1889, from Portugal (Acari, Hydrachnidia, Teutoniidae)
Authors: Pešić Vladimir , Konopleva Ekaterina S. , Iannucci Alessio , Ferreira Sónia , Stur Elisabeth 
Published: February 2025

Water mites of the genus Teutonia Koenike, 1889 are often common in cold montane lakes and pools of shaded streams and include the fastest swimmers among the water mites. This study provides the first-time calibrated phylogeny of this genus in Europe. Fossil-calibrated modelling reveals that the most recent common ancestor of the European Teutonia spp. originated in the Oligocene. Phylogenetic analyses show the presence of at least five species-level lineages within European teutoniid mites, with the T. corsicana-clade as the earliest branching lineage. We infer the origin of Teutonia corsicana at 29.0 million years ago, near the Eocene–Oligocene boundary, indicating that this species or its most recent ancestor was already present on the Corsica-Sardinia block when it broke off from the Iberian Peninsula. The remaining Teutonia clades are relatively younger and began their divergence in the early Miocene (ca. 21 million years ago) and continued with individual species throughout the Miocene. Finally, as a result of an integrative taxonomic approach, three species, i.e., T. lusitanica sp. nov., T. herminiana sp. nov. and T. condei sp. nov. are described as new for science, and the presence of T. cometes is for the first time molecularly confirmed for Portugal.
Antiretrovirus Activity of a Novel Class of Acyclic Pyrimidine Nucleoside Phosphonates
Authors: Balzarini J. , Pannecouque C. , De Clercq E. , Aquaro S. , Perno C.-F. , Egberink H. , Holý A. 
Published: July 2002

ABSTRACT

A novel class of acyclic nucleoside phosphonates has been discovered in which the base consists of a pyrimidine preferably containing an amino group at C-2 and C-4 and a 2-(phosphonomethoxy)ethoxy (PMEO) or a 2-(phosphonomethoxy)propoxy (PMPO) group at C-6. The 6-PMEO 2,4-diaminopyrimidine (compound 1) and 6-PMPO 2,4-diaminopyrimidine (compound 11) derivatives showed potent activity against human immunodeficiency virus (HIV) in the laboratory (i.e., CEM and MT-4 cells) and in primary (i.e., peripheral blood lymphocyte and monocyte/macrophage) cell cultures and pronounced activity against Moloney murine sarcoma virus in newborn NMRI mice. Their in vitro and in vivo antiretroviral activity was comparable to that of reference compounds 9-[(2-phosphonomethoxy)ethyl]adenine (adefovir) and (
R
)-9-[(2-phosphonomethoxy)-propyl]adenine (tenofovir), and the enantiospecificity of (
R
)- and (
S
)-PMPO pyrimidine derivatives as regards their antiretroviral activity was identical to that of the classical (
R
)- and (
S
)-9-(2-phosphonomethoxy)propyl purine derivatives. The prototype PMEO and PMPO pyrimidine analogues were relatively nontoxic in cell culture and did not markedly interfere with host cell macromolecular (i.e., DNA, RNA, or protein) synthesis. Compounds 1 and 11 should be considered attractive novel pyrimidine nucleotide phosphonate analogues to be further pursued for their potential as antiretroviral agents in the clinical setting.
Functional modulation of the human gut microbiome by bacteria vehicled by cheese
Authors: Milani Christian , Longhi Giulia , Alessandri Giulia , Fontana Federico , Viglioli Martina , Tarracchini Chiara , Mancabelli Leonardo , Lugli Gabriele Andrea , Petraro Silvia , Argentini Chiara , Anzalone Rosaria , Viappiani Alice , Carli Elisa , Vacondio Federica , van Sinderen Douwe , Turroni Francesca , Mor Marco , Ventura Marco 
Published: February 2025

ABSTRACT

Since cheese is one of the most commonly and globally consumed fermented foods, scientific investigations in recent decades have focused on determining the impact of this dairy product on human health and well-being. However, the modulatory effect exerted by the autochthonous cheese microbial community on the taxonomic composition and associated functional potential of the gut microbiota of human is still far from being fully dissected or understood. Here, through the use of an
in vitro
human gut-simulating cultivation model in combination with multi-omics approaches, we have shown that minor rather than dominant bacterial players of the cheese microbiota are responsible for gut microbiota modulation of cheese consumers. These include taxa from the genera
Enterococcus
,
Bacillus
,
Clostridium,
and
Hafnia
. Indeed, they contribute to expand the functional potential of the intestinal microbial ecosystem by introducing genes responsible for the production of metabolites with relevant biological activity, including genes involved in the synthesis of vitamins, short-chain fatty acids, and amino acids. Furthermore, tracing of cheese microbiota-associated bacterial strains in fecal samples from cheese consumers provided evidence of horizontal transmission events, enabling the detection of particular bacterial strains transferred from cheese to humans. Moreover, transcriptomic and metabolomic analyses of a horizontally transmitted (cheese-to-consumer) bacterial strain, i.e.,
Hafnia paralvei
T10, cultivated in a human gut environment-simulating medium, confirmed the concept that cheese-derived bacteria may expand the functional arsenal of the consumer’s gut microbiota. This highlights the functional and biologically relevant contributions of food microbes acquired through cheese consumption on the human health.

IMPORTANCE
Diet is universally recognized as the primary factor influencing and modulating the human intestinal microbiota both taxonomically and functionally. In this context, cheese, being a fermented food with its own microbiota, serves not only as a source of nourishment for humans, but also as a source of nutrients for the consumer’s gut microbiota. Additionally, it may act as a vehicle for autochthonous food-associated microorganisms which undergo transfer from cheese to the consumer, potentially influencing host gut health. The current study highlights not only that cheese microbiota-associated bacteria can be traced in the human gut microbiota, but also that they may expand the functional repertoire of the human gut microbiota, with potentially significant implications for human health.
CARPDM: cost-effective antibiotic resistome profiling of metagenomic samples using targeted enrichment
Authors: Hackenberger Dirk , Imtiaz Hamna , Raphenya Amogelang R. , Alcock Brian P. , Poinar Hendrik N. , Wright Gerard D. , McArthur Andrew G. 
Published: February 2025

ABSTRACT

Better interrogation of antimicrobial resistance requires new approaches to detect the associated genes in metagenomic samples. Targeted enrichment is an ideal method for their sequencing and characterization. However, no open-source, up-to-date hybridization probe set targeting antimicrobial resistance genes exists. Here, we describe the Comprehensive Antibiotic Resistance Probe Design Machine (CARPDM), a probe design software package made to run alongside all future Comprehensive Antibiotic Resistance Database releases. To test its efficacy, we have created and tested two separate probe sets: allCARD, which enriches all genes encoded in the Comprehensive Antibiotic Resistance Database’s protein homolog models (
n
= 4,661), and clinicalCARD, which focuses on a clinically relevant subset of resistance genes (
n
= 323). We demonstrate that allCARD increases the number of reads mapping to resistance genes by up to 594-fold. clinicalCARD performs similarly when clinically relevant genes are present, increasing the number of resistance-gene mapping reads by up to 598-fold. In parallel with this development, we have established a protocol to synthesize any probe set in-house, saving up to 350 dollars per reaction. Together, these probe sets, their associated design program CARPDM, and the protocol for in-house synthesis will democratize metagenomic resistome analyses, allowing researchers access to a cost-effective and efficient means to explore the antibiotic resistome.

IMPORTANCE
Antimicrobial resistance threatens to undermine all modern medicine and is driven by the spread of antimicrobial resistance genes among pathogens, environments, patients, and animals. DNA sequencing of complex samples, such as wastewater, shows considerable promise for tracking these genes and making risk assessments. However, these methods suffer from high costs and low detection limits, plus a requirement for frequent redesign due to the constantly evolving diversity of resistance genes. Building upon our Comprehensive Antibiotic Resistance Database, our research provides software for on-demand renewal, based on the latest knowledge of resistance gene diversity, of our novel bait-capture hybridization platform that simultaneously reduces cost and increases detection levels for DNA sequencing of complex samples. The significance of our research is in the development of new software tools, reagent synthesis protocols, and hybridization enrichment protocols to provide affordable, high-resolution metagenomics DNA sequencing, which we test using environmental and wastewater samples.
Effect of fecal microbiota transplantation on diabetic wound healing through the IL-17A–mTOR–HIF1α signaling axis
Authors: Peng Chenmei , Lei Pan , Qi Hongying , Zhu Qianjun , Huang Chushun , Fu Ju , Zhao Chengyu 
Published: February 2025

ABSTRACT

Diabetes is the third most common chronic disorder worldwide. Diabetic wounds are a severe complication that is costly and often results in non-traumatic lower limb amputation. Recent investigations have demonstrated that the gut microbiota as a “virtual organ” can regulate metabolic diseases like diabetes. Fecal microbiota transplantation (FMT) is an innovative therapeutic approach for promoting wound healing, but its function remains incompletely defined. A diabetes model was established by supplying mice with a high-fat diet and performing an intraperitoneal injection of streptozotocin. Diabetic wounds were then created, followed by bacterial transplantation. The relevant indexes of wound healing were evaluated to verify the promoting effect of FMT on the diabetic wounds. Human skin keratinocytes were also cultured, and cell scratch experiments were conducted to further investigate the underlying mechanism. The FMT regulated the levels of specific bacteria in the diabetic mice and helped restore the balance of intestinal microbes. This transplantation also enhanced wound healing in the diabetic mice by augmenting the closure rate, accelerating re-epithelialization, and boosting collagen deposition in skin wounds. Furthermore, FMT promoted the production of IL-17A, which significantly enhanced the growth and movement of human keratinocytes. Inhibiting molecules related to the IL-17A–mTOR–HIF1α signaling axis were shown to hinder wound re-epithelialization.This study clarifies the function of the IL-17A–mTOR–HIF1α signaling axis in the utilization of FMT in diabetic wound healing, providing a new therapeutic method and target for promoting the healing of diabetic wounds.

IMPORTANCE
The Intestinal microbiota, as the organ with the largest number of microorganisms in the body, plays a crucial role in the physiological functions of the human body. Normal microbiota can be involved in various functions such as energy absorption, metabolism, and immunity of the body, and microbiota imbalance is related to many diseases such as obesity and diabetes. Diabetes, as one of the world’s three major chronic diseases, is a significant health issue that troubles more than a billion people globally. Diabetic wounds are a problem that all diabetic patients must confront when undergoing surgery, and it is an important cause of non-traumatic amputations. Exploring the role of intestinal microorganisms in the wound-healing process of diabetic mice can offer the possibility of using microorganisms as a therapeutic means to intervene in clinically related diseases.
LeishmaniaPromastigotes Release a Granulocyte Chemotactic Factor and Induce Interleukin-8 Release but Inhibit Gamma Interferon-Inducible Protein 10 Production by Neutrophil Granulocytes
Authors: van Zandbergen G. , Hermann N. , Laufs H. , Solbach W. , Laskay T. 
Published: July 2002

ABSTRACTRecent data from our laboratory suggest that neutrophil granulocytes (polymorphonuclear leukocytes [PMN]) can serve as host cells forLeishmania majorin the early phase of infection. In line with these findings, an early influx of PMN to the infected tissues was shown by others to be associated with susceptibility to infection withL. major. The mechanisms underlying the initial PMN recruitment to the site of infection is poorly understood. In the present study we investigated whetherLeishmaniacan influence PMN migration. Supernatants ofLeishmaniapromastigotes were tested for their chemotactic activity using an in vitro chemotaxis assay. AllLeishmaniaspecies tested (L. major,L. aethiopica, andL. donovani) displayed a marked chemotactic effect on human PMN. However, no effect on the migration of macrophages and NK cells was observed. Checkerboard analysis revealed that the observed PMN migration was due to chemotaxis rather than chemokinesis. Most of the chemotactic activity was found in fractions containing molecules with sizes between 10 and 50 kDa. Pretreatment of PMN withN-formyl-methionyl-leucyl-phenylalanine blocked the chemotactic activity ofLeishmaniasupernatants up to 75%. In addition, we found that leishmanial contact induced the release of interleukin-8 (IL-8) and inhibited the production of gamma interferon-inducible protein 10 (IP-10) by PMN. These data suggest that infection withLeishmaniapromastigotes leads to PMN accumulation via the production of a chemotactic factor by the parasites, and this effect is amplified by the induction of IL-8 production in PMN. On the other hand, the inhibition of IP-10 production can lead to prevention of NK cell activation.
A single residue in domain II of envelope protein of yellow fever virus is critical for neutralization sensitivity
Authors: Lou Ya-Nan , Sun Meng-Xu , Li Kai , Xiong Xiao-Chuan , Zhou Chao , Cao Tian-Shu , Li Xiao-Feng , Qin Cheng-Feng 
Published: February 2025

ABSTRACT

The live-attenuated yellow fever (YF) vaccine strain 17D (referred to as YF-17D) is the only commercially available vaccine against YF. Recent yellow fever virus (YFV) isolates of South American genotype I (SAI) showed reduced sensitivity to sera from individuals vaccinated with YF-17D. However, the underlying mechanisms remain inadequately elucidated. Here we conducted a screening of a panel of amino acid changes prevalent in domain II of the envelope protein (EDII) of SAI YFV isolates. Each mutation was introduced into the infectious clone of YF-17D utilizing standard reverse genetics. Subsequent neutralization assays demonstrated that the A83E mutant was more sensitive to sera from YF-17D-vaccinated mice and individuals than YF-17D did. Additionally, the A83E mutant displayed neurovirulence and viscerotropic characteristics comparable to those of the parental YF-17D in murine models. Notably, administration of a single dose of the A83E mutant resulted in an enhanced neutralization response against this variant compared to YF-17D and provided complete protection against lethal YFV challenge in mice. Collectively, these findings not only identify a crucial amino acid residue that influences the neutralization sensitivity of YFV but also provide a promising candidate for the development of an updated YF vaccine.

IMPORTANCE
The YF-17D vaccine has been used to prevent YF disease. However, recent strains belonging to the SAI displayed reduced sensitivity to the antibodies produced by vaccination, raising concerns about potential future outbreaks. To identify potential amino acid residues responsible for the decreased neutralizing activity of YF-17D-vaccinated sera, we conducted a screening and generated recombinant viruses with amino acid changes specific to the SAI in EDII using the YF-17D genome as a genetic backbone. We found the A83E mutation played a key role in reducing neutralizing sensitivity to YF-17D-vaccinated mouse sera. Importantly, the A83E mutant maintained a comparable attenuation phenotype to YF-17D but elicited enhanced neutralization activity and conferred protection in mice. Together, we identify a key amino acid residue responsible for the neutralization escape of SAI YFV isolates. We propose that this substitution could act as a target for developing an updated YF-17D vaccine.