bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin increases mitochondrial proteins (VDAC and SDH) and downmodulates AXL and PIM-1 tyrosine kinase receptors in NRAS melanoma cells
Authors: Rocha-Brito Karin J. P. , Clerici Stefano Piatto , Cordeiro Helon Guimarães , Scotá Ferreira Amanda Petrina , Barreto Fonseca Emanuella Maria , Gonçalves Paola R. , Abrantes Júlia Laura F. , Milani Renato , Massaro Renato Ramos , Maria-Engler Silvya Stuchi , Ferreira-Halder Carmen V. 
Published: December 2021

Abstract
Melanoma is a type of skin cancer with low survival rates after it has metastasized. In order to find molecular differences that could represent targets of quercetin in anti-melanoma activity, we have chosen SKMEL-103 and SKMEL-28 melanoma cells and human melanocytes as models. Firstly, we observed that quercetin was able in reducing SKMEL-103 cell viability, but not in SKMEL-28. Besides that, quercetin treatment caused inhibition of AXL in both cell lines, but upregulation of PIM-1 in SKMEL-28 and downregulation in SKMEL-103. Moreover, HIF-1 alpha expression decreased in both cell lines. Interestingly, quercetin was more effective against SKMEL-103 than kinases inhibitors, such as Imatinib, Temsirolimus, U0126, and Erlotinib. Interestingly, we observed that while the levels of succinate dehydrogenase and voltage-dependent anion channel increased in SKMEL-103, both proteins were downregulated in SKMEL-28 after quercetin’s treatment. Furthermore, AKT, AXL, PIM-1, ABL kinases were much more active and chaperones HSP90, HSP70 and GAPDH were highly expressed in SKMEL-103 cells in comparison with melanocytes. Our findings indicate, for the first time, that the efficacy of quercetin to kill melanoma cells depends on its ability in inhibiting tyrosine kinase and upregulating mitochondrial proteins, at least when SKMEL-103 and SKMEL-28 cells response were compared.
Wirtschaft und Recht: Probleme struktureller Kopplung
Authors: Luhmann Niklas 
Published: December 2021

Zusammenfassung
Ausgehend von der Theorie operational geschlossener Systeme und dem damit zusammenhängenden Informationsbegriff kann das Verhältnis solcher Systeme zu ihrer Umwelt über den Begriff der strukturellen Kopplung erläutert werden. Wenn man diese Konzepte auf das Verhältnis von Wirtschaft und Recht anwendet, können klassische Fragestellungen neu analysiert werden: So können Eigentum und Vertrag als evolutionär entstandene gesellschaftliche Einrichtungen der Kopplung beider Systeme verstanden werden, die einerseits Systemzustände und andererseits Systemereignisse koordinieren – und dies mit ganz unterschiedlichen Folgen für beide Systeme. Entsprechend gestaltet sich auch die Entwicklungsgeschichte der entsprechenden Semantiken im Zuge der funktionalen Ausdifferenzierung der Gesellschaft. Die gesellschaftsevolutionär erfolgreiche Etablierung der Kopplungsformen bedeutet aber noch keine Zukunftsgarantie. Insbesondere vor dem Hintergrund der sich ändernden Temporalstrukturen der modernen Gesellschaft in der Form von Technikfolgen, der Ökologiefrage und der Sensibilisierung für Risiken wird vielmehr das geräuschlose Funktionieren der strukturellen Kopplungen, wie es z. B. die liberale Theorie von Eigentum und Vertragsfreiheit postulieren, zunehmend in Frage gestellt.
Komplexität, strukturelle Kontingenzen und Wertkonflikte
Authors: Luhmann Niklas 
Published: December 2021

Zusammenfassung
Temporalisierte, aus Operationen bestehende Systeme sehen sich vor das Problem des Dauerzerfalls gestellt, das sie nur dadurch lösen können, dass die Auswahl nächster Ereignisse hoch selektiv, aber nicht beliebig erfolgt. Sie benötigen dafür ein Gedächtnis. Für den Fall der aus Kommunikation bestehenden Gesellschaft übernimmt die Semantik als bewahrenswerter Sinn diese Funktion. Die hier vorgestellte These lautet, dass die moderne Orientierung an „Werten“ mit der funktionalen Differenzierung der modernen Gesellschaft zusammenhängt, während ältere, vorwiegend hierarchisch stratifizierte Gesellschaften in ganz anderer Weise auf die Selektionszwänge ihrer eigenen Komplexität reagiert hatten. Die mit der funktionalen Differenzierung einhergehende zunehmende Kontingenz macht die Einführung neuer „inviolate levels“ nötig, diesem Bedarf trägt die Wertsemantik Rechnung. Kennzeichnend für die moderne Gesellschaft ist dann, dass Werte als implizites Wissen unterstellt werden und es keine Werthierarchie gibt. Abschließend wird die These der höheren strukturellen Komplexität der funktional differenzierten Gesellschaft auf einer zweiten, konkreteren Ebene noch einmal aufgenommen: der des Wechselspiels der generellen Inklusion der Gesamtbevölkerung in die Funktionssysteme und der nur selektiven Inklusion in Organisationen. Inwiefern unter diesen Bedingungen zunehmend kontingenter Identitäten die Semantik der nicht weiter begründungspflichtigen Werte noch ihre Funktion erfüllt, muss offen bleiben.
The Test Research on the Adsorption of Heavy Metal Ions by Ultra-Low Permeability Polymer-Bentonite with Chemical Resistance Stability
Authors: Cai Xiaoqing , Chen Ying , Liao Rao-ping , Wu Ze-xiang , Yu Chuang , Shi Tiantian , Wu Yinxia , Jiang Jifang 
Published: December 2021

Abstract
It is often difficult for the modification of impermeable bentonite to meet the requirements of low permeability, high chemical resistance, and strong adsorption performance. Based on the low permeability and high chemical resistance of the modified bentonite with sodium polyacrylate (SPB), the adsorption characteristics of the modified bentonite on copper were further compared and analyzed. A series of batch adsorption experiments were performed considering the effects of contact time, pH, temperature, and adsorbate concentration. The morphological changes and microstructure of raw bentonite and SPB at different cupric ion (Cu2+) concentrations were studied through scanning electron microscopy. The pore structure was analyzed with mercury intrusion porosimetry. Kinetic, Langmuir, and Freundlich models were applied to analyze the adsorption mechanism. The results indicated that the SPB not only improves the impermeability and chemical resistance of bentonite but also significantly improves the adsorption of heavy metals. The saturated adsorption capacity of Cu2+ is increased by 116.08 %. The modification mechanism of SPB is that the mineral particles of bentonite were coated by a negatively charged and hydrophilic group (carboxyl group, -COO-). The protective effect of the polymer on the bentonite is the main reason for its chemical resistance, and the solid bonding of the polymer closes the pores of the material, thus reducing the hydraulic performance. Based on the above, it can be concluded that SPB has the characteristics of lower permeability, stronger chemical corrosion resistance, and greater heavy metal adsorption capacity, which makes it an ideal anti-seepage material for landfill.
Reflexiones sobre el alcance del Plan Distrital de Lectura y Escritura IED Villas del Progreso
Authors: Páez Vigoya Doris Adriana 
Published: December 2021

Este artículo tiene el propósito de dar a conocer el alcance que tuvo la implementación del Plan Distrital de Lectura y Escritura -PDLE- en la ciudad de Bogotá, específicamente el proceso de intervención pedagógica realizado en la IED Villas del Progreso, ubicada en la localidad de Bosa.
El PDLE surgió en el año 2016 iniciativa, de la SED como alternativa para mejorar los procesos de lectura y escritura de los niños, y planteó acciones de intervención para los estudiantes de grado tercero en las instituciones con bajo desempeño en las Pruebas Saber de los años 2012, 2014 y 2016. A partir del desarrollo del plan de intervención y acompañamiento pedagógico, se propuso llevar a cabo una fase de diagnóstico con los estudiantes para reconocer las dificultades en habilidades comunicativas, posteriormente, revisar los objetivos propuestos a lo largo de la implementación para conocer el impacto del PDLE in situ. A partir de esta experiencia se reestructuró el plan de estudios de la asignatura de lenguaje que contribuyó a mejorar los diseños pedagógicos de los docentes y fortaleció la formación profesional atendiendo a las necesidades propias del contexto socio-cultural de la institución. En consecuencia, este artículo busca presentar los tipos de participación que se dieron en el marco de la intervención y del proyecto. De igual forma pretende valorar los alcances tanto de los procesos de formación docente como de las experiencias de aula.
Prospects of Development of Retail Trade Formats for Mongolian Goods in the Context of Competition on the Russian Market
Authors: Novikova Nadezhda 
Published: December 2021

The article examined the reasons for the decline in the apparel market, the issue of poor development of trade in Mongolian brand woolen knitwear and its place in fashionable retail. The study made the case for the development of formats that sell such clothing and identified tactual formats for which it is necessary to make changes. It also assessed the competitive position using the coordinate system of M. Porter's typical strategies, as well as the values of the target consumers. We found that brand clothes made from Mongolian woolen knitwear lack clear positioning. We demonstrated the possibilities to change the location and format of trade, and the necessity to employ various models of trade in Mongolian branded knitwear.
Myles Brand’s College Sports Sustainability: “Amateurism”, Finances, and Institutional Balance
Authors: Fort Rodney 
Published: December 2021

As NCAA President, Myles Brand championed three major college sports initiatives: academic integrity, diversity, and sustainability. This paper is about the last. The first step is to distill the elements of college sports that Brand identified repeatedly in his documents and speeches on sustainability. The central elements are the NCAA definition of “amateurism”, athletic department finances, and balance between athletic and academic spending as a part of the university mission. An assessment of these three suggests that NCAA amateurism has changed since his death, in ways Brand stated should raise worries about sustainability. Finances and balance within the university have changed very little over the past ten years and appear sustainable into the future.
Representasi Ayah Ideal dalam Iklan Asuransi MetLife Versi “My Dad's Story Dream for My Child” Tahun 2015
Authors: Udin Muhammad Daffa , Yassar Al-Wafi Muhammad Humam , Firstiansyah Said Zaidaan 
Published: December 2021

Sosok ayah memiliki peran penting di dalam keluarga. Fenomena tersebut diambil oleh Metlife dalam iklannya, yang merepresentasikan seperti apa gambaran ayah yang ideal. Penelitian ini dilakukan untuk mengkaji representasi sosok ayah ideal yang ditunjukkan di dalam iklan asuransi Metlife. Penelitian ini menggunakan teori representasi yang bermakna sebagai proses di mana praktik penandaan tampak untuk berdiri atau mewakili objek atau praktik lain di dunia nyata. Lalu teori idealitas ayah dalam pengasuhan anak yang digambarkan dengan pernyataan yang ada, yaitu ayah direpresentasikan perannya sebagai orang yang secara efektif mencirikan kegiatan untuk membantu tumbuh kembang anak-anak mereka. Metode yang digunakan dalam penelitian ini adalah metode semiotika Roland Bsrthes. Setelah mengiterpretasikan serta mengkaji iklan MetLife mengenai respresentasi ayah ideal, dengan demikian kami memperoleh hasil bahwa representasi ayah yang ideal yang digambarkan dalam iklan tersebut adalah penghibur, penyayang, dan juga bertanggung jawab atas kebahagiaan anaknya.
Concordancias estructurales y otros aspectos relevantes de la regulación del teletrabajo en Argentina y España
Authors: Facundo Temperoni Juan 
Published: December 2021

Ponderando sus principales antecedentes y el particular contexto sanitario en el que han sido concebidas, el presente análisis procura brindar una descripción del núcleo de coincidencias que se registran en las recientes regulaciones del teletrabajo concebidas en la Argentina y España. Con ese objetivo, medianteel cotejo pertinente, y sin soslayar algunos de sus rasgos distintivos, se enuncian las diferentes aristas que en común exhiben los dos regímenes destinados a normar la nueva forma de organización del trabajo, en lo cardinal, aquellos derechos y directrices fundamentales como son la igualdad en reflejo con las personas que trabajan presencialmente, la voluntariedad, reversibilidad y desconexión digital, entre otros.
Adaptasi Beberapa Varietas Kedelai di Bawah Tegakan Tanaman Sawit Muda
Authors: Zainudin Zainudin , Mustikarini Eries Dyah , Aini Sitti Nurul 
Published: December 2021

<p>Kedelai merupakan salah satu komoditas yang dapat dimanfaatkan sebagai tanaman sela pada perkebunan tanaman kelapa sawit muda. Namun, rendahnya intensitas cahaya akibat naungan sering menjadi kendala untuk mencapai hasil tinggi. Penelitian bertujuan untuk mengetahui varietas kedelai yang toleran dan memiliki daya hasil tinggi bila dibudidayakan di bawah naungan tegakan tanaman sawit muda (umur ± 2,5 tahun). Penelitian dilaksanakan pada bulan Januari hingga April 2020 di Desa Petaling, Kecamatan Mendo Barat, Kabupaten Bangka menggunakan rancangan petak terpisah, tiga ulangan. Petak utama adalah kondisi lahan ternaungi dan tidak ternaungi. Anak petak terdiri atas lima varietas kedelai yaitu: Argomulyo, Anjasmoro, Grobogan, Burangrang, dan Demas 1. Hasil penelitian menunjukkan bahwa pada tingkat naungan ±38%, varietas Demas 1 dan Anjasmoro lebih toleran dibandingkan varietas Argomulyo, Burangrang dan Grobongan. Varietas kedelai toleran terhadap naungan menghasilkan jumlah polong, jumlah biji, dan bobot biji per tanaman lebih tinggi dibandingkan varietas yang tidak toleran naungan. <br /><br /></p>
Optimization of Process Parameters on Surface Hardness and Energy Consumption in Milling of 7050 Aluminum Alloy using Enhanced NSGA-II
Authors: Yang Yang , Su Chen , Wang Hongsen , Wang Yuan , Shu Leshi 
Published: December 2021

Abstract
Aluminum alloy has high strength and light weight. It is widely used for aircraft fuselage, propellers and other parts which work under high load conditions. High-quality parts made of aluminum alloy processed by computerized numerical control (CNC) machine often have the characteristics of high cost in their processing. In order to achieve high surface quality and control processing costs, this article takes the workpiece surface hardness and machining energy consumption as targets. Intelligent optimization algorithm is used to find the optimal combination of milling parameters to obtain ideal targets. CNC milling parameter optimization is a multi-parameter, multi-objective, multi-constraint, discrete nonlinear optimization problem which is difficult to solve. For this challenge, an improved NSGA-II is presented, named enhanced population diversity NSGA-II (EPD-NSGA-II). EPD-NSGA-II is improved with the normal distribution crossover, adaptive mutation operator of differential evolution, crowding calculation method considering variance and modified elite retention strategy to achieve enhanced population diversity. 12 test functions are chosen for experimentation to verify the performance of the EPD-NSGA-II. The values of three evaluation indicators show that the proposed approach has good distribution and convergence performance. Finally, the approach is applied in the milling parameters optimization of 7050 aluminum alloy to get the optimal solutions. Results indicate that the EPD-NSGA-II is effective in optimizing the problem of milling parameters.
Bacterial over-expression of functionally active human CT2 (SLC22A16) carnitine transporter
Authors: Galluccio Michele , Mazza Tiziano , Scalise Mariafrancesca , Sarubbi Maria Chiara , Indiveri Cesare 
Published: December 2021

Abstract
I. Background: E. coli is a widely used tool for the over-expression of human proteins for studying structure and function. The toxicity of human proteins for E. coli often hampers the expression. This study aims to find conditions for the expression of a membrane transporter known as the carnitine transporter CT2. The knowledge on this transporter is scarce, thus obtaining the recombinant protein is very important for further studies. II. Methods and Results: The cDNAs coding for human CT2 (hCT2) was cloned in the pH6EX3 vector and different transformed E. coli strains were cultured in absence or in presence of glucose. hCT2 expression was obtained. The protein was purified and reconstituted into proteoliposomes in a functionally active state.III. Conclusions: using the appropriate IPTG concentration, together with the addition of glucose, hCT2 has been expressed in E. coli. The protein is active and shows capacity to transport carnitine in proteoliposomes. The results have a great interest in basic biochemistry of membrane transporters and applications to human health since hCT2 is involved in human pathology.
The role of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in the assessment of fibrosis in children with chronic hepatitis C
Authors: Tajiri Hitoshi , Suzuki Mitsuyoshi , Bessho Kazuhiko , Ito Yoshinori , Murakami Jun , Hatori Reiko , Brooks Stephen , Takano Tomoko , Miyoshi Yoko 
Published: December 2021

Abstract
BackgroundAt present non-invasive fibrosis markers are not available for assessment of liver fibrosis in children with chronic hepatitis C. MethodsSixty-three children with chronic hepatitis C were included. Changes in Wisteria floribunda agglutinin-positive Mac-2 binding protein (M2BPGi) levels were evaluated in l3 of 27 treatment-naive patients during the observation period of the natural course of disease (median 4, range 3-6 years). Changes during treatment were evaluated in 27 of 36 patients for 4 (2-9) years of post-treatment follow-up. ResultsThere were significant differences in the levels of M2BPGi between the control group and HCV F0 group (P=0.002) and between the control group and HCV F1 group (P<0.001). Receiver operating characteristic curve analysis showed that to detect stage F1 fibrosis cut off value was 0.95 for M2BPGi with sensitivity 52%, specificity 90%, and area under the curve 0.687. A substantial decrease in M2BPGi levels by treatment was shown from 0.98±0.57 at pre-treatment to 0.42±0.15 at post-treatment (P<0.001) in the 27 treated-patients. ConclusionsOur study shows new findings that M2BPGi may be useful to predict the presence of a mild degree of fibrosis in children with chronic hepatitis C and such a mild fibrosis may be quickly resolved by antiviral therapies.
Molecular Detection of Bacterial Contamination in Blood Components Using Magnetic-based Enrichment
Authors: Lee Jinyeop , Abafogi Abdurhaman Teyib , Oh Sujin , Chang Ho Eun , Tepeng Wu , Lee Daekyu , Park Sungsu , Park Kyoung Un , Hong Yun Ji 
Published: December 2021

Abstract
Bacterial contamination of blood products is a major problem in transfusion medicine, in terms of both morbidity and mortality. Platelets (PLTs) are stored at room temperature (under constant agitation) for more than 5 days, and bacteria can thus grow significantly from a low level to high titers. However, conventional methods like blood culture and lateral flow assay have disadvantages such as long detection time, low sensitivity, and the need for a large volume of blood components. We used real-time polymerase chain reaction (PCR) assays with antibiotic-conjugated magnetic nanobeads (MNBs) to detect enriched Gram-positive and -negative bacteria. The MNBs were coated with polyethylene glycol (PEG) to prevent aggregation by blood components. Over 80% of all bacteria were captured by the MNBs, and the levels of detection were 101 colony forming unit [CFU]/mL and 102 CFU/mL for Gram-positive and -negative bacteria, respectively. The detection time is < 3 h using only small volumes of blood components. Thus, compared to conventional methods, real-time PCR using MNBs allows for rapid detection with high sensitivity using only a small volume of blood components.
Panax Notoginseng Saponins Reverse Steroid Resistance in Lupus Nephritis: Involvement of the Suppression of Exosomal P-gp Levels From Lymphocytes to Glomerular Endothelial Cells
Authors: Pan Feng , Lu Ying 
Published: December 2021

Abstract
Backgrounds: Microangiopathy is the most basic pathological manifestation of lupus nephritis (LN), and glomerular endothelial cells (GECs) injury is an important pathological mechanism. LN patients with microangiopathy are prone to steroid resistance (SR). Our previous studies confirmed that Panax notoginseng saponins (PNS) could reverse SR by downregulating the expression of P-gp in SR lymphocytes of LN mice (SLCsL/S). However, the mechanism of how circulating lymphocytes transmit SR information to GECs and thus affect the efficacy of kidney treatment is not clear. Recent studies have found that exosomes (exos) are an important carrier for intercellular bioactive substance communication. The aim of the study is to investigate whether exosomes derived from SLCsL/S mediate SR in GECs and PNS intervention.Methods: Exosomes isolated from SLCsL/S were characterized, and in vitro cell coculture was further conducted to investigate the effect of SLCsL/S-derived exosomes in the SR of GECs and PNS intervention. Sequencing was used to define the exosomal miRNA expression profiling of SR GECs. Moreover, the in vivo experiments were performed through the injection of exosomes extracted from SLCsL/S into the tail vein of mice.Results: In this study, we showed that exosomes derived from SLCsL/S could transmit SR information to GECs and lead to the aggravation of inflammatory injury through conferring P-gp, which were negated by a P-gp inhibitor. Further, we identified higher levels of exosomal miR-125b-5p from SR GECs were associated with SR in LN and could serve as biomarker for the risk of developing SR. PNS could reverse the SR of GECs and alleviate inflammatory injury by suppressing exosomal P-gp levels from lymphocytes to GECs in vitro and in vivo.Conclusions: Our findings suggest that exosomal transfer of SLCsL/S derived P-gp confer SR to GECs, and PNS can target exosome communication to reverse SR in LN, which provides new ideas and a scientific basis for improving the clinical efficacy of traditional Chinese medicine in the treatment of refractory LN.
Comparison of the Prognostic Value of Global and Regional Myocardial Flow Capacity Radius, Myocardial Flow Reserve, and Stress Myocardial Blood Flow Using Rubidium-82 with SiPM PET/CT
Authors: DIETZ Matthieu , Kamani Christel H , Allenbach Gilles , Rubimbura Vladimir , Fournier Stephane , Lalonde Marie Nicod , Schaefer Niklaus , Eeckhout Eric , Muller Olivier , Prior John O 
Published: December 2021

Abstract
Purpose

The aim of this study was to assess the most reliable quantitative variable on Rubidium-82 (82Rb) cardiac PET/CT for predicting major adverse cardiovascular events (MACE), on the latest PET camera using silicon photomultipliers digital readout (SiPM) technology.
Methods

We prospectively enrolled 274 consecutive participants with suspected myocardial ischemia. Participants underwent 82Rb cardiac SiPM PET/CT and were followed-up for MACE over 652 days (interquartile range: 559 to 751 days). For each participant, global and regional myocardial flow reserve (MFR), stress myocardial blood flow (stress MBF) and their combination as myocardial flow capacity radius (MFC radius) were measured.
Results

On receiver operator curve analysis, MACE prediction was similar for global and regional MFR, stress MBF, and MFC radius (area under the curve; (i) Global: 0.70 vs. 0.71 and 0.73, and (ii) Regional: 0.71 vs. 0.71 and 0.73, respectively, p > 0.1 for all pairwise comparisons). On multivariable analysis, (i) Global: MFR < 1.98, stress MBF < 1.94 mL/g/min, and MFC radius < 3.12, as well as (ii) Regional: MFR < 1.75, stress MBF < 1.75 mL/g/min, and MFC radius < 2.7, emerged all as similar independent predictors of MACE (p < 0.001 for all).
Conclusions

Using the latest SiPM PET technology with 82Rb, global and regional MFR, stress MBF, and MFC radius are similar powerful predictors of cardiovascular event.