bluuebunny/crossref_metadata_2025_split huggingface dataset:
Membrane nanotubes are ancient machinery for cell-to-cell communication and transport. Their interference with the immune system
Authors: Matkó János , Tóth Eszter Angéla 
Published: February 2021

AbstractNanotubular connections between mammalian cell types came into the focus only two decades ago, when “live cell super-resolution imaging” was introduced. Observations of these long-time overlooked structures led to understanding mechanisms of their growth/withdrawal and exploring some key genetic and signaling factors behind their formation. Unbelievable level of multiple supportive collaboration between tumor cells undergoing cytotoxic chemotherapy, cross-feeding” between independent bacterial strains or “cross-dressing” collaboration of immune cells promoting cellular immune response, all via nanotubes, have been explored recently. Key factors and "calling signals" determining the spatial directionality of their growth and their overall in vivo significance, however, still remained debated. Interestingly, prokaryotes, including even ancient archaebacteria, also seem to use such NT connections for intercellular communication. Herein, we will give a brief overview of current knowledge of membrane nanotubes and depict a simple model about their possible “historical role”.
Dual rolls equal channel extrusion as unconventional SPD process of the ultralow-carbon steel: finite element simulation, experimental investigations and microstructural analysis
Authors: Jabłońska M. B. , Kowalczyk K. , Tkocz M. , Bulzak T. , Bednarczyk I. , Rusz S. 
Published: February 2021

Abstract
The paper presents results of FEM modelling as well as properties and microstructure of the ultralow-carbon ferritic steel after the unconventional SPD process—DRECE (dual rolls equal channel extrusion). Based on the conducted numerical simulation information about the deformation behaviour of a steel strip during the DRECE process was obtained. The simulation results were experimentally verified. The influence of DRECE process on hardness distribution, fracture behaviour and microstructure evolution of the investigated steel was analysed. The increase of steel strength properties after subsequent deformation passes was confirmed. The microstructural investigations revealed that the processed strips exhibit the dislocation cell microstructure and subgrains with mostly low-angle grain boundaries. The grains after processing had relatively high dislocation density and intense microband formation was observed. It was also proved that this unconventional SPD method fosters high grain refinement.
Paving the way to achieving the United Nations Sustainable Development Goals for women from Indigenous communities: lessons from Attappady, India
Authors: Thomas Sarah T. , Thomas Elizabeth T. , McLean Michelle , Titus Thomas T. 
Published: February 2021

AbstractIndia is home to the world’s second largest Indigenous population, comprising 8.6% of the national population. They are constitutionally recognised as ‘Scheduled Tribes’ to aid their development after centuries of oppression and socio-cultural marginalisation through the caste system. Limited disaggregated data exist on India’s Scheduled Tribe populations’ health outcomes, including for Indigenous women. Kerala, one of India’s southern states, is an intriguing case study. The State has outperformed other Indian states and South Asian countries with respect to a number of health and education indicators despite its more modest economic performance. Relatively little is known, however, about whether the State’s tribal or ‘Adivasi’ population is prospering. This article used data from a cross-sectional observational study of tribal women conducted in the Attappady area in the Palakkad district in Kerala, South India, which has a dense tribal population. The outcomes for these communities were compared with the relevant United Nations 2030 Sustainable Development Goal (SDG) indicators as well as Kerala State data to identify areas of growth and need. The findings of this case study highlight successes as well as persisting gaps in health outcomes for women and children in marginalised tribal communities. Using a strengths-based approach, we propose possible strategies to address the perceived gaps.
Economic Evaluation of Investments in Airports: Recent Developments
Authors: Forsyth Peter , Niemeier Hans-Martin , Njoya Eric Tchouamou 
Published: February 2021

AbstractThe problem of how to evaluate investments in airports has now been studied for over 50 years. This paper analyzes the use of different methods like cost–benefit analysis (CBA), economic impact analysis (EIA), and computable general equilibrium (CGE) models to address the question. It assesses the strength and weaknesses of each method, and it discusses which methods have been used in different countries. The paper argues that the CBA approach and the newer CGE modeling approach address the policy issue well and that both methods are appropriate, although improvements are possible, especially in the newer aspects of evaluation. Furthermore, more data intensive CGE models are able to analyze broader aspects of the evaluation question for which CBA has had difficulty. EIA does not address the problem satisfactorily, and it misleads air transport policy. But this evaluation contrasts sharply with practice. EIA has been extensively used to decide on airport investment. CGE approaches are very promising, though further work is needed for them to reach their full potential. This paper pays particular attention to the relationship between CBA and CGE in airport investment evaluation and also the possible role of wider economic benefits (WEBs) of aviation in evaluation.
Neural Antibody Testing for Autoimmune Encephalitis: A Canadian Single-Centre Experience
Authors: Budhram Adrian , Mirian Ario , McFadden Sean , Edmond Pamela , Bhayana Vipin , Yang Liju 
Published: February 2021

ABSTRACT:
Neural antibodies have emerged as useful biomarkers in suspected autoimmune encephalitis. We reviewed results of neural antibody testing (anti-N-methyl D-aspartate receptor (NMDAR), leucine-rich glioma-inactivated protein (LGI1), contactin-associated protein-like 2 (CASPR2), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR), γ-aminobutyric acid type B receptor (GABA(B)R), dipeptidyl-peptidase-like protein-6 (DPPX), IgLON family member 5 (IgLON5) and glutamic acid decarboxylase-65 (GAD65)) using cell-based assays (CBAs) and tissue indirect immunofluorescence (TIIF) at our centre. Our findings suggest increased clinical sensitivity of CBA compared to TIIF. However, this may come at some expense to clinical specificity, as evidenced by possible false-positive results when weak serum positivity by CBA was observed for certain antibodies (i.e. anti-NMDAR, CASPR2). In such cases, correlation with serum TIIF, as well as CSF CBA and TIIF, aids in identifying true-positive results.
Hydrogen peroxide vapor decontamination of N95 respirators for reuse
Authors: Kenney Patrick A. , Chan Benjamin K. , Kortright Kaitlyn E. , Cintron Margaret , Russi Mark , Epright Jacqueline , Lee Lorraine , Balcezak Thomas J. , Havill Nancy L. , Martinello Richard A. 
Published: February 2021

AbstractObjective:The coronavirus disease 2019 (COVID-19) pandemic has led to global shortages of N95 respirators. Reprocessing of used N95 respirators may provide a higher filtration crisis alternative, but whether effective sterilization can be achieved for a virus without impairing respirator function remains unknown. We evaluated the viricidal efficacy of Bioquell vaporized hydrogen peroxide (VHP) on contaminated N95 respirators and tested the particulate particle penetration and inhalation and exhalation resistance of respirators after multiple cycles of VHP.Methods:For this study, 3M 1870 N95 respirators were contaminated with 3 aerosolized bacteriophages: T1, T7, and Pseudomonas phage phi-6 followed by 1 cycle of VHP decontamination using a BQ-50 system. Additionally, new and unused respirators were sent to an independent laboratory for particulate filter penetration testing and inhalation and exhalation resistance after 3 and 5 cycles of VHP.Results:A single VHP cycle resulted in complete eradication of bacteriophage from respirators (limit of detection 10 PFU). Respirators showed acceptable limits for inhalation/exhalation resistance after 3 and 5 cycles of VHP. Respirators demonstrated a filtration efficiency >99 % after 3 cycles, but filtration efficiency fell below 95% after 5 cycles of HPV.Conclusion:Bioquell VHP demonstrated high viricidal activity for N95 respirators inoculated with aerosolized bacteriophages. Bioquell technology can be scaled for simultaneous decontamination of a large number of used but otherwise intact respirators. Reprocessing should be limited to 3 cycles due to concerns both about impact of clinical wear and tear on fit, and to decrement in filtration after 3 cycles.
Towards a definitive symptom structure of obsessive−compulsive disorder: a factor and network analysis of 87 distinct symptoms in 1366 individuals
Authors: Cervin Matti , Miguel Euripedes C. , Güler Ayşegül Selcen , Ferrão Ygor A. , Erdoğdu Ayşe Burcu , Lazaro Luisa , Gökçe Sebla , Geller Daniel A. , Yulaf Yasemin , Başgül Şaziye Senem , Özcan Özlem , Karabekiroğlu Koray , Fontenelle Leonardo F. , Yazgan Yankı , Storch Eric A. , Leckman James F. , do Rosário Maria Conceição , Mataix-Cols David 
Published: February 2021

AbstractBackgroundThe symptoms of obsessive−compulsive disorder (OCD) are highly heterogeneous and it is unclear what is the optimal way to conceptualize this heterogeneity. This study aimed to establish a comprehensive symptom structure model of OCD across the lifespan using factor and network analytic techniques.MethodsA large multinational cohort of well-characterized children, adolescents, and adults diagnosed with OCD (N = 1366) participated in the study. All completed the Dimensional Yale-Brown Obsessive−Compulsive Scale, which contains an expanded checklist of 87 distinct OCD symptoms. Exploratory and confirmatory factor analysis were used to outline empirically supported symptom dimensions, and interconnections among the resulting dimensions were established using network analysis. Associations between dimensions and sociodemographic and clinical variables were explored using structural equation modeling (SEM).ResultsThirteen first-order symptom dimensions emerged that could be parsimoniously reduced to eight broad dimensions, which were valid across the lifespan: Disturbing Thoughts, Incompleteness, Contamination, Hoarding, Transformation, Body Focus, Superstition, and Loss/Separation. A general OCD factor could be included in the final factor model without a significant decline in model fit according to most fit indices. Network analysis showed that Incompleteness and Disturbing Thoughts were most central (i.e. had most unique interconnections with other dimensions). SEM showed that the eight broad dimensions were differentially related to sociodemographic and clinical variables.ConclusionsFuture research will need to establish if this expanded hierarchical and multidimensional model can help improve our understanding of the etiology, neurobiology and treatment of OCD.
Dynamic Analysis of the UVMS: Effect of Disturbances, Coupling, and Joint-Flexibility on End-Effector Positioning
Authors: Shah Umer Hameed , Karkoub Mansour , Kerimoglu Deniz , Wang Hong-Du 
Published: February 2021

SUMMARYThis paper investigates the dynamics of an underwater vehicle-manipulator system (UVMS) consisting of a two-link flexible-joint manipulator affixed to an autonomous underwater vehicle. The quasi-Lagrange formulation is utilized in deriving a realistic mathematical model of the UVMS considering joints’ friction, hysteretic coupling between the joints and links, and the nonlinear hydrodynamic forces acting on the system, such as added mass, viscous damping, buoyancy, drag, and vortex-induced forces. Numerical simulations are performed to demonstrate the effects of hydrodynamic forces and system coupling between the vehicle and the manipulator and the joints and the links on the precise positioning of the end effector.
Economic impact of the use of the National Surgical Quality Improvement Program
Authors: Fansi Alvine , Ly Angela , Mayrand Julie , Wassef Maggy , Rho Aldanie , Beauchamp Sylvie 
Published: February 2021

Objectives
The American College of Surgeons National Surgical Quality Improvement Program (ACS NSQIP®) is a validated, risk-adjusted database for improving the quality and security of surgical care. ACS NSQIP can help participating hospitals target areas that need improvement. The aim of this study was to systematically review the literature analyzing the economic impact of using NSQIP. This paper also provides an estimation of annual cost savings following the implementation of NSQIP and quality improvement (QI) activities in two hospitals in Quebec.

Methods
In June 2018, we searched in seven databases, including PubMed, Embase, and NHSEED for economic evaluations based on NSQIP data. Contextual NSQIP databases from two hospitals were collected and analyzed. A cost analysis was conducted from the hospital care perspective, comparing complication costs before and after 1 year of the implementation of NSQIP and QI activities. The number and the cost of complications are measured. Costs are presented in 2018 Canadian dollars.

Results
Out of 1,612 studies, 11 were selected. The level of overall evidence was judged to be of moderate to high quality. In general, data showed that, following the implementation of NSQIP and QI activities, a significant decrease in complications and associated costs was observed, which improved with time. In the cost analysis of contextual data, the reduction in complication costs outweighed the cost of implementing NSQIP. However, this cost analysis did not take into account the costs of QI activities.

Conclusions
NSQIP improves complication rates and associated costs when QI activities are implemented.
Atomized urbanism: secrecy and security from the Gulag to the Soviet closed cities
Authors: Siddiqi Asif 
Published: February 2021

AbstractThis article recovers the early history of the Soviet ‘closed city’, towns that during the Cold War were absent from maps and unknown to the general public due to their involvement in weapons research. I argue that the closed cities echoed and appropriated features of the Stalinist Gulag camp system, principally their adoption of physical isolation and the language of obfuscation. In doing so, I highlight a process called ‘atomized urbanism’ that embodies the tension between the obdurate reality of the city and the goal of the state to obliterate that reality through secrecy. In spatial terms, ‘atomized’ also describes the urban geography of these cities which lacked any kind of organic suburban expansion.
Hyperlactataemia as a predictor of adverse outcomes post-cardiac surgery in neonates with congenital heart disease
Authors: Valencia Eleonore , Staffa Steven J. , Nathan Meena , Smith-Parrish Melissa , Kaza Aditya K. , DiNardo James A. , Nasr Viviane G. 
Published: February 2021

AbstractObjective:To evaluate the discriminative ability of hyperlactataemia for early morbidity and mortality in neonates with CHD following cardiac surgery.Methods:Retrospective, observational study of neonates who underwent cardiac surgery on cardiopulmonary bypass at a tertiary care children’s hospital from June 2015 to June 2019. The primary predictor was lactate. The primary composite outcome was defined as ≥1 of the following: cardiac arrest or extracorporeal membrane oxygenation within 72 hours or 30-day mortality post-operatively. The secondary outcome was the presence of major residual lesions, according to the Technical Performance Score.Results:Of 432 neonates, 28 (6.5%) sustained the composite outcome. On univariate analysis, peak lactate within 48 hours, increase in lactate from ICU admission through 12 hours, and single ventricle physiology were significantly associated with the composite outcome. The peak lactate occurred at a median of 2.9 hours (interquartile range: 1, 35) before the event. Through multi-variable analysis, a multi-variable risk algorithm was created. Predicted probabilities demonstrated an increasing risk based on single ventricle status and delta lactate, ranging from 1.8% (95% CI: 0.9, 3.9) to 52.4% (95% CI: 32.4, 71.7). The model had good discriminative ability for the composite outcome on receiver operating characteristic analysis (area under the curve = 0.79; 95% CI: 0.75, 0.89). Moreover, a peak lactate of 7.3 mmol/l or greater was significantly associated with the presence of a major residual lesion (odds ratios: 5.16, 95% CI: 3.01, 8.87).Conclusions:We present a simple, two-variable model, including delta lactate in the immediate post-operative period and single ventricle status, to prognosticate the risk of early morbidity and mortality in neonates undergoing cardiac surgery for potential intervention.
Impact of CT-based planning and bladder volume changes on the dosimetry of vaginal vault brachytherapy
Authors: Venkatasai Jeyaanth , Ramireddy Jebakarunya , Sathyamurthy Arvind , Godson Henry Finlay , Karuppusami Reka , Das Saikat , Ram Thomas Samuel 
Published: February 2021

AbstractAim:To compare dose to target and organs at risk (OARs) in conventional plan (2D) versus computed tomography (CT)-based three-dimensional (3D) plan in vaginal cuff brachytherapy (VBT) and to compare the effect of bladder distension on target and OARs dosimetry.Materials and methods:Post-hysterectomy patients with an indication for VBT were included in the study. All patients underwent planning CT scans with a full bladder and an empty bladder protocol. For each CT, two plans were generated—one library-based 2D plan and another CT-based 3D plan. Dosimetric parameters were recorded for clinical target volume (CTV) and OARs.Results:A total of 92 observations were made from data collected from 46 patients. Difference between CTV dose in terms of 2D and 3D plans were not statistically significant for CTV (p = 0·11). Significant reduction in D0·1cc, D1cc and D2cc dose parameters were observed in bladder, rectum, sigmoid and bowel doses with the 3D plan (p < 0·001). Bladder distension showed a 20% reduction in dose for bowel (p < 0·001). Bladder distension also showed a 6·12% (p = 0·047) increase in D2cc, but there was a significant reduction in the mean dose to the bladder.Conclusion:Our study demonstrates the dosimetric benefits with 3D CT-based planning for VBT over 2D-based conventional planning and benefit of bladder distension in the reduction of bowel dose without compromising dose to the target volume.
Stroke rate after thoracic endovascular aortic repair using de-airing of stentgrafts with high-volume of saline solution
Authors: Branzan Daniela , Geisler Antonia , Steiner Sabine , Lautenschlaeger Tina , Doss Markus , Matschuck Manuela , Scheinert Dierk , Schmidt Andrej 
Published: February 2021

Summary: Background: Our aim was to determine the rate of ischemic stroke following thoracic endovascular aortic repair (TEVAR) after reducing gas volume released during stentgraft deployment by de-airing of thoracic stentgrafts with high-volume of 0.9% heparinized saline solution. Patients and methods: A single center retrospective analysis of all consecutive patients undergoing TEVAR from 2014 to 2019 was performed. All thoracic stentgrafts were flushed with 120 ml 0.9% heparinized saline solution before implantation, according to our institutional protocol. Endpoints were in-hospital rates of ischemic stroke and spinal cord ischemia (SCI), and all-cause mortality. Results: One hundred and fifty-four patients (mean age: 66.8 ± 13.6 years, 64.9% males) were treated with TEVAR during the study period. Indications for treatment were thoracic aortic aneurysms (n = 75, 48.7%), acute type B aortic dissections (n = 46, 29.9%), aortic arch aneurysms and penetrating aortic ulcers (n = 28, 18.2%), and blunt traumatic aortic injuries (n = 5, 3.2%). Timing of procedure was urgent in 75 patients (48.7%). Proximal landing zone were zone 0–1–2 (n = 75, 48.7%), zone 3 (n = 66, 42.9%) and zone 4 (n = 13, 8.4%). Supra-aortic vessels were revascularized with custom-made fenestrated stentgrafts in 9 patients (5.8%), using chimney technique in 4 patients (2.6%), and with debranching procedures in 19 patients (12.3%). Left subclavian artery was covered without revascularization in 46 patients (29.9%). In-hospital stroke occurred in two patients (1.3%) and SCI in another two patients (1.3%). In-hospital mortality rate was 0.6%. No further in-hospital events were noted. Conclusions: De-airing of stentgrafts with high-volume of 0.9% heparinized saline solution seems to be safe and can be used as an adjunct to keep occurrence of neurological events after TEVAR as low as possible.
The Deep Structure of the Alps Based on the CIFALPS Seismic Experiment: A Synthesis
Authors: Malusà M. G. , Guillot S. , Zhao L. , Paul A. , Solarino S. , Dumont T. , Schwartz S. , Aubert C. , Baccheschi P. , Eva E. , Lu Y. , Lyu C. , Pondrelli S. , Salimbeni S. , Sun W. , Yuan H. 
Published: February 2021

AbstractThe European Alps are the site where classic geologic concepts such as nappe theory, continental subduction and slab breakoff have been first proposed. However, the deep tectonic structure of the Alps has long been poorly constrained by independent geophysical evidence. This review paper summarizes the main results of the CIFALPS passive seismic experiment, that was launched by Chinese, French and Italian scientists in the 2010s to provide new insights on the deep tectonic structure of the Alpine region. The application of a wide range of tomographic methods to the analysis of a single fossil subduction zone makes the CIFALPS experiment a potential reference case for the analysis of other orogenic belts. Major results include: (i) the first seismic evidence of European continental crust subducted into the Adriatic upper mantle, beneath the place where coesite was first recognized in continental (U)HP rocks in the Alps; (ii) evidence of a major involvement of the mantle wedge during (U)HP rock exhumation; (iii) evidence of a serpentinized plate interface favoring continental subduction; (iv) evidence of a continuous slab beneath the Western and Central Alps, ruling out the classic model of slab breakoff magmatism; (v) evidence of a polyphase development of anisotropic fabrics in the Alpine mantle, either representing active mantle flows or fossil fabrics inherited from previous rifting stages. Detection of these major tectonic features allows to propose interpretive geologic cross‐sections at the scale of the lithosphere and upper mantle, providing a baseline for future analyses of active continental margins.
SRF is a nonhistone methylation target of KDM2B and SET7 in the regulation of skeletal muscle differentiation
Authors: Kwon Duk-Hwa , Kang Joo-Young , Joung Hosouk , Kim Ji-Young , Jeong Anna , Min Hyun-Ki , Shin Sera , Lee Yun-Gyeong , Kim Young-Kook , Seo Sang-Beom , Kook Hyun 
Published: February 2021

AbstractThe demethylation of histone lysine residues, one of the most important modifications in transcriptional regulation, is associated with various physiological states. KDM2B is a demethylase of histones H3K4, H3K36, and H3K79 and is associated with the repression of transcription. Here, we present a novel mechanism by which KDM2B demethylates serum response factor (SRF) K165 to negatively regulate muscle differentiation, which is counteracted by the histone methyltransferase SET7. We show that KDM2B inhibited skeletal muscle differentiation by inhibiting the transcription of SRF-dependent genes. Both KDM2B and SET7 regulated the balance of SRF K165 methylation. SRF K165 methylation was required for the transcriptional activation of SRF and for the promoter occupancy of SRF-dependent genes. SET7 inhibitors blocked muscle cell differentiation. Taken together, these data indicate that SRF is a nonhistone target of KDM2B and that the methylation balance of SRF as maintained by KDM2B and SET7 plays an important role in muscle cell differentiation.