bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Constraint of the Eco-Friendly Rooftop PV System Implementation Based on the Techno-economic and Regulatory Impact Analysis: Case Study of The Ontowiryo Mosque in Purworejo, Central Java, Indonesia
Authors: Suparwoko Suparwoko - , Qamar Fadhil Ahmad 
Published: April 2021

Abstract
Background: The government is optimistic that Indonesia is able to be energy independent because Indonesia has a variety of energy sources that can be utilized for the benefit of the nation and state, both fossil and non-fossil energy sources, including solar energy through solar power system. The solar power system or photovoltaic system is an eco-friendly option usually applied to meet the need for energy due to its lower carbon footprint when compared to fossil-fueled power plants. This research was, therefore, used to conduct a techno-economic and regulatory impact analysis on a rooftop photovoltaic system installed in a mosque in Purworejo, Indonesia under a net metering mechanism. Result: The installation was aimed at reducing entire carbon emission from the annual energy usage which is an equivalent of 4 tons of carbon dioxide. The research shows that the economic feasibility of the photovoltaic systems, measured by the Net Present Value (NPV) of the electricity bill saving are negative values for all the configurations based on the type and number of panels. This low financial attractiveness was observed to be due to several unsupportive regulations such as subsidized electricity tariff, minimum grid electricity usage limit, and less-rewarding net metering scheme. The research also suggests that the abolishment of the minimum usage limit was discovered to have incurred the greatest increase of NPV to the photovoltaic system designed to entirely supply the load demand while the implementation of a higher electricity tariff caused the highest increase of NPV for the photovoltaic systems with partial supply scenario. Conclusion: Finally, it is financially unfeasible to install a photovoltaic system on the mosque rooftop at the current electricity tariff, costs of components, and regulations implemented concerning the adoption of the system. As a constraint, the power regulation in Indonesia should be reviewed significantly to support any buildings of worship to develop their opportunities for managing energy systems independently. The potential implication is that they would be able to contribute their social economic role in supporting their really crucial community daily life energy. This approach is in line with the government policy and strengthening the sustainable development goals.
Emissions inventory for road transport in India in 2020: Framework and post facto policy impact assessment
Authors: Singh Namita , Mishra Trupti , Banerjee Rangan 
Published: April 2021

Abstract
India's growing population and economic development lead to an increase in transport emissions. Quantification of emissions at frequent intervals is required to assess the emission levels and impact of control policies implemented. Implemented policies affect the fleet configuration over time. Therefore, in the present paper, an age-wise emission analysis framework was developed for the road transport sector with updated fleet characteristics corresponding to the vehicles' age. The results show that fuel consumption is estimated to be 92 (87–95) Mt, and total CO2, CO, PM, and NOx vehicle emissions are estimated to be 274 (265–292) Tg, 4463 (3253–6676) Gg, 164 (119–250) Gg, and 2378 (2191–3045) Gg, respectively for the reference year 2020. The study contributes by developing an inventory for the fleet of 2020 used as a benchmark to compare past emissions inventories, evaluate control policies, estimate state-wise vehicle emissions inventories, and identify significant emitters in the fleet. Sensitivity analysis indicates the considerable variation in total emissions resulting from different age-mix of vehicles. Among the investigated policies, advancement in emission norms followed by fuel efficiency improvement in vehicles led to a substantial reduction in gaseous pollutants. Based on the inventory results, suitable policies are suggested for India's future fleet, and the need for country-level fleet characteristics data is recommended.
A randomized controlled study of anterior lumbar plexus combined with parasacral sciatic nerve block under the guidance of ultrasound and nerve stimulator in elderly patients undergoing hip arthroplasty
Authors: Wu Wei , Bu Wen-hao , Huang Hai-bin , Mao Ying , Tan Yong-xing 
Published: April 2021

Abstract
Objectives: To explore the clinical application of anterior lumbar plexus combined with parasacral nerve block under the dual guidance of ultrasound and nerve stimulator in elderly hip arthroplasty.Methods: Forty elderly patients aged from 65 to 85 years old who underwent unilateral hip arthroplasty (Except for revision of hip joint) were randomly divided into two groups (n=20 in each group): ultrasound-guided anterior lumbar plexus combined with parasacral sciatic nerve block group (group A) and ultrasound-guided anterior lumbar plexus block group (group B). Both groups were assisted with sedation with different doses of dexmetopyrimidine and analgesia with different doses of sufentanil. Because the orthopaedic team of our hospital performed hip arthroplasty with mature technique and fixed operation time, the onset time of block in group An and B, heart rate and blood pressure at admission (T0), skin incision (T1), half hour after skin incision (T2) and skin suture(T3), intraoperative dosage ofdexmedetomidine and sufentanil, postoperative 1, 4, 8, 16, 24hVAS score and the first time of getting out of bed were recorded.Results: the heart rate and blood pressure at T1, T2 and T3 in group A were significantly lower than those in group B (P < 0.05). The amount of sufentanil used during operation in group), A was significantly lower than that in group B (P < 0.05). The postoperative scores of 1,4,8,16 and 24hVAS in group), A were significantly lower than those in group B (P < 0.05), and the time of getting out of bed for the first time after operation was shorter than that in group B (P < 0.05).Conclusion: compared with simple anterior lumbar plexus block, anterior lumbar plexus combined with parasacral sciatic nerve block in elderly patients with hip arthroplasty can make the intraoperative circulation more stable, the intraoperative dosage of opioids significantly reduced and the effect of early postoperative analgesia is better. it can also shorten the time for patients to get out of bed for the first time.
Age-related ceRNA networks and the mRNA/protein correlation in Drosophila ageing
Authors: Yang Deying , Yuan Ya , He Zhi , Tu Jianbo , Wang Siqi , Li Jiamei , Zhang Pu , Mao Xueping , Fan Xiaolan , Li Diyan , Zeng Bo , Ni Qingyong , Li Yan , Zhang Mingwang , Yan Taiming , Yang Mingyao 
Published: April 2021

Abstract
Background

As Drosophila is a classic model organism, understanding of the regulatory networks has great significance in revealing the genetic mechanisms of ageing and human diseases. Competing endogenous RNA (ceRNA)-mediated regulation is an important mechanism of circular RNA (circRNA) and long non-coding RNA (lncRNA) regulation in ageing and age-related disease. However, extensive analyses of the multi-omics (circRNA/miRNA/mRNA, lncRNA/miRNA/mRNA, and mRNA/protein) characteristics of adult Drosophila ageing have not been reported. Our results determine the ceRNA networks and the mRNA/protein correlations of key differentially expressed genes in adult Drosophila aging, and provide a solid foundation for understanding mechanisms of human ageing and ageing-related diseases.
Results

Here, differentially expressed circRNAs and microRNAs (miRNAs) between 7 and 42 day old Drosophila were screened and identified. Then, the differentially expressed mRNAs, circRNAs, miRNAs, lncRNAs, and proteins between day 7 and day 42 were used to analyse fly age-related circRNA/miRNA/mRNA and lncRNA/miRNA/mRNA networks and the mRNA/protein correlation in Drosophila ageing. Several key ceRNA networks were identified, such as the circ_0006913/miR-985-3p/Abl, circ_0009500/miR-14-5p/SERCA, XLOC_100429/miR-14-5p/SERCA, and XLOC_100429/miR-14-5p/Vha100-4 networks. Subsequently, 16 pairs of differentially expressed interacting mRNAs/proteins were found, including 10 pairs with the same expression trends and 6 pairs with the opposite expression trends. Furthermore, real-time quantitative PCR (qPCR) was used to verify the expression level of those genes.
Conclusions

The discovery of these ceRNA networks and the mRNA/protein correlations in adult Drosophila ageing will provide new information for research on human ageing and age-related disease.
Coherent optical communications using coherence-cloned Kerr soliton microcombs
Authors: Geng Yong , Zhou Heng , Han Xinjie , Cui Wenwen , Zhang Qiang , Liu Boyuan , Guang-Wei Deng , Zhou Qiang , Qiu Kun 
Published: April 2021

Abstract
Dissipative Kerr soliton microcomb has been recognized as a promising on-chip multi-wavelength laser source for fiber optical communications, as its comb lines possess frequency and phase stability far beyond the independent lasers. In the scenarios of coherent optical transmission and interconnect, a highly beneficial but rarely explored target is to re-generate a Kerr soliton microcomb at the receiver side as local oscillators that conserve the frequency and phase property of the incoming data carriers, so that to enable coherent detection with minimized optical and electrical compensations. Here, by using the techniques of pump laser conveying and two-point locking, we implement re-generation of a Kerr soliton microcomb that faithfully clones the frequency and phase coherence of another microcomb sent from 50 km away. Moreover, leveraging the coherence-cloned soliton microcombs as carriers and local oscillators, we demonstrate terabit coherent data interconnect, wherein traditional digital processes for frequency offset estimation is totally dispensed with, and carrier phase estimation is substantially simplified via slowed-down phase estimation rate per channel and joint phase estimation among multiple channels. Our work reveals that, in addition to providing a multitude of laser tones, regulating the frequency and phase of Kerr soliton microcombs among transmitters and receivers can significantly improve optical coherent communication in terms of performance, power consumption, and simplicity.
Direct Biotic Plant–soil Feedback Promotes Dodonaea Viscosa Growth in a Dry-hot Valley, China
Authors: Wang Xuemei , Yan Bangguo , Shi Liangtao , Liu Gangcai 
Published: April 2021

Abstract
Biotic plant-soil feedback has been widely studied, and may be particularly important in resource-poor areas. However, the roles of soil nutrient cycling in affecting plant growth in this process still remained unclear. The aim of this study was to explore the roles of soil biota in regulating nutrient cycling by conducting a two-phase feedback experiment in a dry-hot valley, with a conditioning phase during which there were Dodonaea viscosa or no D. viscosa growing in the soil, and a feedback phase in which the effect of the conditioned soil biota on D. viscosa performance was measured. The growth of D. viscosa significantly reduced soil N after the conditioning phase. However, D. viscosa showed a positive plant-soil feedback. In the feedback phase, the D. viscosa conditioned soil promoted the stem diameter, leaf area, and leaf dry mass content of D. viscosa. Total biomass was also significantly higher in D. viscosa conditioned soil than that in not conditioned soil. In contrast, soil sterilization had a negative effect on the growth of D. viscosa, with a significant reduction in plant biomass, especially in D. viscosa conditioned soil, and soil sterilization significantly increased the root: shoot biomass ratio and litter mass. Furthermore, we showed that although the biota-driven changes in enzyme activities correlated with the leaf N and P amount especially P amount, the enzyme activity was not the main reason to promote D. viscosa growth in the conditioned soil.
A Novel Entomopathogenic Actinobacteria Mediated Silver Nanoparticles Characterization and Their Insecticidal, Antibacterial and Cytotoxic Activities
Authors: Raguvaran Krishnan , Kalpana Manickam , Manimegalai Thulasiraman , Balakrishnan Rengasamy , Maheswaran Rajan 
Published: April 2021

Abstract
Nanomaterials were highly inspired in the field of nanotechnology especially bio synthesized silver nanoparticles (AgNPs) have effectively attracted worldwide that helps to improve in the field of medical science and human health. Actinabacteria mediated nanoparticle were widely used to control insect pests, treatment of antibiotic resistant bacteria, cancer and other diseases due to their potential pharmacological properties compared with other chemical drugs. The present investigation an entomopathogenic actinobacteria Actinokineospora fastidiosa was isolated and synthesis of AgNPs and tested their mosquito larvicidal, antibacterial and anticancer activity. Moreover, the outcome of biosynthesized AgNPs was characterized by UV-visible spectroscopy, X-ray Diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), Transmission electron microscope (TEM), scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDX) and zeta potential analysis. The characterized AgNPs showed a potent larvicidal activity against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus the obtained LC50 values is 9.86 ppm, 8.50 ppm and 8.29 ppm respectively. However, at 25 ppm concentration, AgNPs showed highest ovicidal activity (100 %, 100 % and 97.77 % against A. aegypti, A. stephensi and C. quinquefasciatus respectively) and oviposition deterrence activity was 100 %, 100 % and 98.66 % were achieved against A. aegypti, A. stephensi and C. quinquefasciatus respectively. The enzymes of α, β esterase and Glutathione-S-transferase level were increased due to detoxification process against AgNPs. The histopathological results of AgNPs showed series damage in epithelial cells and brush border cell of mosquito larval gut. In addition, actinobacterial mediated AgNPs was tested against different human pathogenic bacteria. The obtained results showed biosynthesized AgNPs revealed remarkable antibacterial activity against all tested pathogens. The lowest mortality was observed on non-target organisms of Artemia salina at highest concentration of AgNPs. Finally, in vitro toxicity study showed that biosynthesized AgNPs potential anticancer activity and induced reactive oxygen species (ROS) production in human cervical cancer (HeLa) cell line at an inhibitory concentration (IC50) range 42.37 μg/mL. Therefore, it can be concluded that biosynthesized AgNPs using a novel stains Actinokineospora fastidiosa potentially influenced the vector mosquito and bacterial diseases management and pharmacological applications in eco-friendly manner.
Silencing of Sphingosine 1-Phosphate Receptors and Potassium Channels May Inhibits the Invasion and Migration of Breast Cancer
Authors: Cosan Didem Turgut , SOYOCAK Ahu , ÇALIŞ İbrahim Uğur 
Published: April 2021

Abstract
Molecular receptor signaling mechanisms play an important role in many pathophysiological processes, including breast cancer. The spread of cancer from peripheral tissue to distant organs by metastasis is the cause of death of most breast cancer patients. For that reason, the most important step in the treatment of cancer is to prevent metastasis. Sphingosine-1-phosphate receptors and potassium channels play role of cancer cell migration, invasion and they may interact with each other in the progression of cancer. In this study, it was aimed to determine the effects of combined silencing of receptors and channels on the invasion and migration of MCF-7 and MDA-MB-231 breast cancer cells and their interactions on cells. We examined the expression levels of S1P1, S1P3, Kv1.3, and Kv10.1 in MCF-7 and MDA-MB-231 breast cancer cell lines by qRT-PCR. The effects of migration and invasion of breast cancer cells were determined through invasian and wound healing assays. It was observed that high invasion and lateral motility in cells decreased with the combined silencing of S1P1, S1P3, Kv1.3 and Kv10.1 in both cell types. It has been determined that silencing the receptors and channels together is more effective than silencing individually. Our data demonstrated the roles of S1P receptors and potassium channels were associated with invasion and migration signaling pathway. Therefore, these are might be possible therapeutic target for breast cancer metastasis.
Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress
Authors: Ju huining , Li daxing , Li Dequan , Yang Xinghong , Liu Yang 
Published: April 2021

Abstract
Late embryogenesis abundant (LEA) proteins are widely assumed to play crucial roles in environmental stress tolerance, but their function has remained obscure. Dehydrins are group II LEA proteins, which are highly hydrophilic plant stress proteins. In the present study, a novel group II LEA protein, ZmDHN11 was cloned and identified from maize. The expression of ZmDHN11 was induced by high osmotic stress, low temperature, salinity and ABA (abscisic acid). The ZmDHN11 protein specifically accumulated in the nuclei and cytosol. Further study indicated that ZmDHN11 is phosphorylated by the casein kinase CKII. ZmDHN11 protected the activity of LDH under water deficit stress. The overexpression of ZmDHN11 endows transgenic yeast and tobacco with tolerance to osmotic stress.
High Performance Liquid Chromatography – Ultraviolet Method for the Determination of Fludioxonil Fungicide Residues: Application on Rice Grains Cultivated in Pakistan
Authors: Khalid Aneeqa , Ali Saeeda Nadir , Qayoom Amtul , Iqbal Sajid , Ansari Sadia , Awan Zahoor Ul Hussain , Kishwar Farah , Daniel Philippe 
Published: April 2021

Abstract
An analytical method was developed and validated for the determination of fludioxonil in rice samples. Rice samples for the study were collected from different regions of Pakistan. The method was based on safe and cost-effective extraction of fludioxonil from rice grains using acetone and methanol (1:1), efficient clean-up through homogenous mixture of acidic aluminum (12 g) and activated charcoal (1 g) followed by liquid chromatographic determination with UV detection. Quantification was performed on Prospher Star C18 (5 µm, 25 x 0.46 cm) column maintaining the temperature 40ºC and detector wavelength 212 nm using mobile phase 50:50 v/v methanol-water (pH 3.3) employing flow rate 1.0 mL.min-1 and 20 µL injection volume. The method showed linearity with correlation coefficient greater than 0.998. The proposed method was precisely validated for rice sample of all regions, showing recoveries higher than 98%. Rice samples collected from Badin, Multan, Hyderabad, Lahore, Jahania and Sarghoda was found to have fludioxonil residues 0.046, 0.045, 0,043, 0.040, 0.024 and 0.016 mg.kg-1 respectively, all below the maximum residual limit (MRL) level i.e. 0.05 mg.kg-1 whereas samples collected from Khanewal and Gularchi showed fludioxonil residue above MRL i.e 0.065 and 0.058 mg.kg-1 respectively. However, fludioxonil residues was not detected in rice sample collected from city Makhdumpur.
Living with Type 2 Diabetes Mellitus in the Kingdom of Tonga: A Qualitative Investigation of the barriers and enablers to lifestyle management
Authors: Taumoepeau Jennifer , Knight-Agarwal Cathy , Tu'i 'Esiteli , Jani Rati , Osuagwu Levi , Simmons David 
Published: April 2021

Abstract
Background: Despite the increasing prevalence of Type 2 Diabetes Mellitus in the Kingdom of Tonga little is known of the experiences of adults living with this non-communicable disease. This investigation aimed to explore the barriers and enablers to healthy lifestyles in a group of men and women living with type 2 diabetes mellitus residing in this pacific island nation.Methods: This qualitative study consisted of three semi-structured focus groups (n=16) which were conducted at Tonga’s only Public Hospital located in the capital, Nuku’alofa. Discussions were audio recorded, transcribed, cross-checked for consistency, and entered into a word processing document for analysis. Thematic analysis was employed to synthesise results.Results: Four main themes were identified: (1) Knowledge and Support; (2) Fear and Motivation; 3) Physical and Psychological Environment; and (4) Faith and Culture. Conclusion: The qualitative findings from this study may assist the future development and dissemination of culturally appropriate lifestyle-related information for men and women living with Type 2 Diabetes Mellitus in the Kingdom Tonga. The need for collaboration between practitioners at the hospital, the church, family members and local traditional healers is important if the lifestyle-related needs and wants of this group of people are to be met.
Overabundance of Asaia and Serratia bacteria is associated with deltamethrin insecticide susceptibility in Anopheles coluzzii from Agboville, Côte d’Ivoire
Authors: Pelloquin Bethanie , Kristan Mojca , Edi Constant , Meiwald Anne , Clark Emma , Jeffries Claire L. , Walker Thomas , Dada Nsa , Messenger Louisa A 
Published: April 2021

Abstract
Background Insecticide resistance among mosquito species is now a pervasive phenomenon, which threatens to jeopardise global malaria vector control efforts. Evidence of links between the mosquito microbiota and insecticide resistance is emerging, with significant enrichment of insecticide degrading bacteria and enzymes in resistant populations. Using 16S rRNA amplicon sequencing, we characterised and compared the microbiota of Anopheles (An.) coluzzii in relation to their deltamethrin resistance and exposure profiles. Results Comparisons between 2-3 day old deltamethrin resistant and susceptible mosquitoes, demonstrated significant differences in microbiota diversity (PERMANOVA, pseudo-F = 19.44, p=0.0015). Ochrobactrum, Lysinibacillus and Stenotrophomonas genera, each of which comprised insecticide degrading species, were significantly enriched in resistant mosquitoes. Susceptible mosquitoes had a significant reduction in alpha diversity compared to resistant individuals (Shannon index: H=13.91, q=0.0003, Faith’s phylogenetic diversity: H=6.68, q=0.01), with Asaia and Serratia dominating microbial profiles. There was no significant difference in deltamethrin exposed and unexposed 5-6 day old individuals, suggesting that insecticide exposure had minimal impact on microbial composition. Serratia and Asaia were also dominant in 5-6 day old mosquitoes, regardless of exposure or phenotype, and had reduced microbial diversity compared with 2-3 day old mosquitoes. Conclusions Our findings revealed significant alterations of An. coluzzii microbiota associated with deltamethrin resistance, highlighting the potential for identification of novel microbial markers for insecticide resistance surveillance. qPCR detection of Serratia and Asaia was consistent with 16S rRNA sequencing, suggesting that population level field screening of the bacterial microbiota may be feasibly integrated into wider resistance monitoring if reliable and reproducible markers associated with phenotype can be identified.