bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature Sensitivity Analysis of Extended Source Double Gate Tunnel Field Effect Transistor
Authors: Nishad Dharmender , Nigam Kaushal , Kumar Satyendra 
Published: April 2021

Abstract
Temperature-induced performance variation is one of the main concerns of the conventional stack gate oxide double gate tunnel field-effect transistor (SGO-DG-TFET). In this regard, we investigate the temperature sensitivity of extended source double gate tunnel field-effect transistor (ESDG-TFET). For this, we have analyzed the effect of temperature variations on the transfer characteristics, analog/RF, linearity and distortion figure of merits (FOMs) using technology computer aided design (TCAD) simulations. Further, the temperature sensitivity performance is compared with conventional SGO-DG-TFET. The comparative analysis shows that ESDG-TFET is less sensitive to temperature variations compared to the conventional SGO-DG-TFET. Therefore, this indicates that ESDG-TFET is more reliable for low-power, high-frequency applications at a higher temperature compared to conventional SGO-DG-TFET.
Phenolic compounds and biological activities of phenolic extract of olive oil mill wastewater issue from the cold extraction of olive oil from Khenchela (Algeria)
Authors: Gueboudji Zakia , Kadi Kenza , Nagaz Kamel , Addad Dalila , Secrafi Mansour , Yahya Leila Ben , Lachehib Belgacem 
Published: April 2021

Abstract
This study aimed to the determination of antioxidant, anti-inflammatory, and anticoagulant activities of phenolic compounds present in olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern of Algeria.After polyphenols extraction, a quantity and quality analysis by LC-MS was made. The LC-MS (liquid chromatography–mass spectrometry)results were revealed the presence of 20 phenolic compounds in theextract of OMW which were: (quinic acid, gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, rutin, transfrolic acid, hyperoside (quercetin-3-o-galactoside), luteolin-7-o-glucoside, naringin, 4.5-di-caffeoyquinic acid, quercetrin(quercetin-3-o-rhamonosid), apegenin-7-o-glucoside, salviolinic acid, kampherol, quercetin, naringenin, apegenin,cirsiliol, and cirsilineol). The results of antioxidant activity with DPPH(2,2-diphenyl-1-picrylhydrazyl), ABTS+ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing ability of plasma)showed that the IC50(half-maximal inhibitory concentration) were(9.62 ± 0.28µg/mL, 7.10 ± 0.11µg/mL and 3.59 ± 0.24µg/mL)respectively. The extract of OMW was found to exhibit the highestinhibitory effectanti-inflammatory activity usinginhibition of protein denaturation(IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81; 87.43 ± 0.66 µg/mL) more than the standard used. In addition, the extract had the best anticoagulation activity in the endogenous and exogenous pathways (44.77 ± 0.25s; 15.84 ± 0.12s). Based on these results, it is right to conclude that OMW is an important source of natural phenolic compounds that have important antioxidant, anti-inflammatory, and anticoagulant activities.
Activation Ferroptosis Enhanced the Therapy Sensitivity of TNBC to Paclitaxel Via NCOA4 Mediated Ferritinophagy
Authors: Qiu Yu , Yu Qifeng , Ji Meng , Zhang Zhiwei , Kang Liuyun , Fu Ying , Feng Miao 
Published: April 2021

Abstract
BackgroundPaclitaxel (PTX) is the first-line drug for chemotherapy, but Triple-Negative Breast Cancer (TNBC) develops resistance to PTX is the main challenge in clinical therapy. Ferroptosis is a new type of regulated cell death (RCD) characterized by iron-dependent lipid peroxidation accumulation, despite activation of ferroptosis has been shown to enhance the sensibility of tumor to chemotherapy drugs, the underlying mechanism remains unclear. MethodsC11-BODIPY probe and real time PCR was used to detect the ferroptosis level in breast cancer cells. FerroOrange probe and western blot was employed to measure ferritinophagy. The cell viability was examined by CCK-8, EdU and AM/PI double staining.ResultsThe anti-cancer effect of PTX was related to the induction of ferroptosis in TNBC cells, the ferroptosis occurring was mediated by NCOA4-driven ferritinophagy, and the autophagy-related molecules also downregulated in clinical specimens. Furtherly, TNBC cells survival was decreased more significantly than PTX alone when combining with ferroptosis agonist RSL3. In addition, we also discovered that HERC2, a unique identified E3 ubiquitin ligase of NCOA4, obviously expressed downregulation under PTX treated TNBC cells while elevated in breast carcinoma comparing with adjacent tissues. Meanwhile, bioinformatics results showed that the survival rate was significantly lower in breast cancer patients with high expression of HERC2.ConclusionsFerroptosis agonist can assist PTX to enhance the sensitivity of TNBC to PTX in chemotherapy, which possible mechanism is prompting the expression of NCOA4 and the occurrence of ferritinophagy.
An Energy Efficient Secure Data Aggregation in Wireless Sensor Networks
Authors: A Jenice Prabu , D Hevin Rajesh 
Published: April 2021

Abstract
In Wireless sensor network, the major issues are security and energy consumption. There may be several numbers of malicious nodes present in sensor networks. Several techniques have been proposed by the researchers to identify these malicious nodes. WSNs contain many sensor nodes that sense their environment and also transmit their data via multi-hop communication schemes to the base station. These sensor nodes provides power supply using battery and the energy consumption of these batteries must be low. Securing the data is to avoid attacks on these nodes and data communication. The aggregation of data helps to minimize the amount of messages transmitted within the network and thus reduces overall network energy consumption. Moreover, the base station may distinguish the encrypted and aggregated data based on the encryption keys during the decryption of the aggregated data. In this paper, two aspects of the problem is concerned, we investigate the efficiency of data aggregation: first, how to develop cluster-based routing algorithms to achieve the lowest energy consumption for aggregating data, and second, security issues in wsn. By using Network simulator2 (NS2) this scheme is simulated. In the proposed scheme, energy consumption, packet delivery ratio and throughput is analyzed. The proposed clustering, routing, and protection protocol based on the MCSDA algorithm shows significant improvement over the state-of - the-art protocol.
Fear of COVID-19 Reinforces Climate Change Beliefs. Evidence From 28 European Countries
Authors: Hortay Oliver , Stefkovics Ádám 
Published: April 2021

Abstract
The long-term nature of climate policy measures requires stable social legitimacy, which other types of crises may jeopardize. This article examines the impact of the COVID-19 fear on climate change beliefs based on an autumn 2020 population survey in the Member States of the European Union and the United Kingdom. The results show that deep COVID-19 concerns increase awareness, climate change concerns, and perceived negative impacts of climate change. These effects are more robust among the lower educated Europeans. On the country level, strict governmental measures are also linked to deep climate change concerns. In contrast to the experience following the 2008 recession, the findings show that a secondary crisis can positively impact climate attitudes, which is a promising result for policy actions.
Pattern of Genetic Inheritance of Grain Zinc and Iron Content, Agronomic and Biochemical Traits in Bread Wheat Using Mixed Linear Models Under Salinity Stress
Authors: Holasou Hossein Abbasi , Kia Seyed Siamak Alavi , Mohammadi Seyed Abolghasem , Vahed Mohammad Moghaddam 
Published: April 2021

Abstract
Wheat is the most important staple food that acts as a primary source of dietary calories, protein and most of the bioavailable micronutrients such as iron (Fe) and zinc (Zn) for the world’s population. Understanding genetic control of micronutrients uptake is necessary for development of good quality wheat genotypes. To study the nature of inheritance of Zn and Fe efficient uptake under none-saline and saline conditions, two Iranian facultative wheat variety; Navid (salt sensitive, Fe and Zn-deficient) and Roshan (salt tolerant, Fe and Zn-efficient); were crossed to generate six basic generations for generation mean analysis. All the genotypes of these six generations were evaluated for grain Zn and Fe content, agronomic and biochemical traits under none-saline and saline conditions. For all the studied traits, the non-additive components were greater than the additive component. Additive effects were negative and significant for all traits under non-saline and saline conditions, except for 100-SW in control, MDA in 100 mM salinity level, EL and LeaNaC in all salinity levels. Additive gene actions were important for grain Zn and Fe content; while for rest of traits both fixable and non-fixable genetic effects were important. Duplicate dominant type of epistasis was involved in inheritance of all the traits. Broad-sense heritability values (>0.6) for most traits under non-saline and saline conditions were high, whereas the narrow-sense heritability values for most of the studied traits were low to moderate. These Zn and Fe efficient uptake indices could be used to select Zn/Fe-efficient wheat genotypes from segregating populations.
Age- and gender-dependent association of SLC11A1 polymorphisms with tuberculosis susceptibility
Authors: Wang Yuhe , Wang Zhongtao , He Xue , Wang Li , Bai Mei , Yuan Dongya , He Yongjun , Jin Tianbo 
Published: April 2021

Abstract
Background: Tuberculosis (TB) is an important health issue in our world. It is reported that various factors may effect on its pathogenesis. In this current study, we aimed to investigate the association between SLC11A1 polymorphism and the risk of TB among 510 TB patients and 508 healthy controls.Methods: Agena MassARRAY platform was conducted for genotyping. Odds ratios (ORs) and 95% confidence intervals (CIs) were analyzed through unconditional logistic regression adjustment confound factors, such as age and gender.Results: The results suggested that the allele and genotype frequencies of polymorphisms in SLC11A1 were not observed associated with TB risk. Subsequently, stratified analysis by age and gender confirmed that rs7608307 “A/A” and “C/T-T/T” genotypes were related with increased TB risk in age ≤ 41 group (p = 0.021) and males (p = 0.013), respectively. Besides, rs13062 “A/A” genotype was reduced TB risk in age > 41 group (p = 0.043). In addition, we observed that the “C/C” genotype of rs4674301 was noteworthy correlated with increased TB risk in females (p = 0.043). Conclusion: Our results demonstrated the relationship between SLC11A1 polymorphism and TB risk and confirmed for the first time that the correlation was restricted to age and gender in northwest Chinese population.
Identification of Prognostic miRNAs Targeting EZH2 in Hepatocellular Carcinoma Using The Cancer Genome Atlas Database
Authors: Lin Tianyu , Guo Xinli , Du Qian , Liu Wei , Zhong Xin , Wang Suihan , Cao Liping 
Published: April 2021

Abstract
Background: Enhancer of zeste homolog 2 (EZH2) gene have a prognostic role in hepatocellular carcinoma (HCC). This study aimed to identify the prognostic microRNAs (miRNAs) targeting EZH2 in HCC. Methods and Results: We downloaded the gene and miRNA RNA-seq data from The Cancer Genome Atlas (TCGA) database. Differences in EZH2 expression between tumor and control samples and those between tumors with different clinical variables were analyzed using the Mann-Whitney U test. Association of EZH2 expression with prognosis in HCC patients was detected using Cox regression analysis. We also identified miRNAs targeting EZH2 with negative correlations, compared the miRNA expression profiles between tumor and control tissues, and identified pathways and protein-protein interaction pairs related to EZH2. The miRNA-EZH2-pathway network was constructed accordingly. EZH2 was significantly upregulated in HCC tumors compared with control samples (p<0.0001) and in tumors with advanced T classifications (3/4 vs. 1/2, p=0.0039) and stages (III/IV vs. I/II, p=0.0028). The Cox regression analysis showed that TCGA HCC patients who had high EZH2 expression levels showed a short survival time (HR=1.677, 95% CI 1.316-2.137; p<0.0001). Among miRNAs targeting EZH2, seven miRNAs, including hsa-let-7c-5p, were negatively correlated with EZH2 expression and were significantly downregulated in HCC tumor samples compared with controls (p<0.0001). The miRNA-EZH2-pathway network included seven downregulated miRNAs and four pathways, including hsa00310: Lysine degradation. Hsa-let-7c-5p was associated with prognosis in HCC (HR=0.849 95% CI 0.739-0.975; p=0.021). Conclusions: EZH2-hsa-let-7c-5p has a significant association with HCC prognosis and the mechanism worth investigating.
Bioinformatics Analysis Predicts hsa_circ_0026337/miR-197-3p as a Potential Oncogenic ceRNA Network for Non-small Cell Lung Cancers
Authors: Zhang Qian , Kang Lingkai , Li Xiaoyue , Li Zhirui , Wen Shimin , Fu Xi 
Published: April 2021

Abstract
BackgroundCircular RNAs (circRNAs) play an essential role in developing tumors, but their role in non-small cell lung cancer (NSCLC) is unclear. Thus, the present study explored the possible molecular mechanism of circRNAs in NSCLC.MethodsThree circular RNA (circRNA) microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database. Differential expressions of circRNAs (DECs) were identified in NSCLC tissue and compared to adjacent healthy tissue. The online cancer-specific circRNA database(CSCD) was used for the analysis of the DECs function. Protein-protein interaction (PPI) network, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), Cytoscape and UALCAN were used to predict the critical nodes and perform patient survival analysis, respectively. The interaction between the DECs, the predicted miRNAs, and hub genes was also determined. Finally, the circRNA-miRNA-mRNA network was established.ResultsThe expression of hsa_circ_0049271, hsa_circ_0026337, hsa_circ_0043256, and hsa_circ_0008234 was decreased in NSCLC tissues. The Encyclopedia of RNA Interactomes (ENCORI) and CSCD database results showed that hsa_circ_0026337 was found to sponge with miR-1193, miR-197-3p, miR-3605-5p, miR-433-3p and miR-652-3p, and hsa_circ_0043256 to sponge with miR-1252-5p, miR-494-3p and miR-558 respectively. Subsequently, 100 mRNAs were predicted to bind with these seven miRNA response elements (MREs). The GO analysis and KEGG pathway revealed that these 100 MREs might be involved in “histone deacetylase binding” and “cellular senescence”. PPI network and Cytoscape identified the top ten hub genes. Survival analysis data showed that the low expression of hsa_circ_0026337 was significantly associated with shortened survival time in NSCLC (P=0.037) ,which increased the expression level of hsa-miR-197-3p, thereby inhibiting the translation of specific proteins.ConclusionThis study examined the circRNA-miRNA-mRNA regulatory network associated with NSCLC and explored the potential functions of DECs in the network to elucidate the mechanisms underlying disease progression in NSCLC.
A Generalized Analysis of Energy Saving Strategies Through Experiment for CNC Milling Machine Tools
Authors: Feng Chunhua , Chen Xiang , Zhang Jingyang , Huang Yugui 
Published: April 2021

Abstract
This paper proposes the elaboration model of energy requirement prediction taking into account the power of standby, spindle rotation in non-load, feeding and rapid movement in X, Y, Z+ and Z- axially, and specific energy consumption (SEC) in the X and Y cutting directions respectively, which could not be considered completely in other models. Each part energy of specific machine tools could be obtained through little experiments for identifying the relationship between energy and tool path with cutting parameters. The method is validated by 27 trial cutting experiment in X and Y cutting directions in VMC850E machine, the results show that the SEC in the X and Y cutting directions are exactly different. Moreover, it is found that spindle power should be piecewise linear representation according to spindle speed characteristic, due to the correlation coefficient of power model only has 25.45% without segmented. Additionally, the correlation coefficient of improved SEC model could reach to more than 99.98% in each segment. The contribution of this paper is mainly the elaboration energy consumption model considering the cutting direction, which is an efficient approach for predicting energy consumption through tool path to achieve sustainable production in manufacturing sectors.
Genomic Insights Into Antibiotic Resistance Pattern of Tetracycline Degrading Bacterium Arthrobacter Nicotianae OTC-16
Authors: Zhang Xin , Zhu Rongrong , Li Weilin , Ma Junwei , Lin Hui 
Published: April 2021

Abstract
Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC 16, which shows substantial biodegradation of oxytetracycline/tetracycline. The results showed that this strain could be resistant to at least seven categories, 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC 16 contains one chromosome(3,643,989 bp) and two plasmids (plasmid1,123,894 bp and plasmid2, 29,841bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During oxytetracycline (OTC) degradation by the strain OTC 16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene(tetV) in the chromosome were tracked by real time quantitative reverse transcription polymerase chain reaction(qRT PCR). Only the plasmid derived resistance genes were up regulated under the pressure of OTC. The presence of OTC increased the tolerance of strain OTC 16 to streptomycin sulphate. This work deepens our understanding of the behavioural characteristics of resistance genes and adaptive evolution of resistant drug bacteria under the pressure of antibiotics.
Human Chromosome 3p21.3 Carries TERT&nbsp; Transcriptional Regulators in Pancreatic Cancer
Authors: Yagyu Takuki , Ohira Takahito , Shimizu Ryutaro , Morimoto Masaki , Murakami Yuki , Hanaki Takehiko , Kihara Kyoichi , Matsunaga Tomoyuki , Yamamoto Manabu , Tokuyasu Naruo , Sakamoto Teruhisa , Fujiwara Yoshiyuki , Kugoh Hiroyuki 
Published: April 2021

Abstract
Frequent loss of heterozygosity (LOH) on the short arm of human chromosome 3 (3p) region has been found in pancreatic cancer (PC), which suggests the likely presence of tumor suppressor genes in this region. However, the functional significance of LOH in this region in the development of PC has not been clearly defined. The human telomerase reverse transcriptase gene (hTERT) contributes to unlimited proliferative and tumorigenicity of malignant tumors. We previously demonstrated that hTERT expression was suppressed by the introduction of human chromosome 3 in several cancer cell lines. To examine the functional role of putative TERT suppressor genes on chromosome 3 in PC, we introduced an intact human chromosome 3 into the human PK9 and murine LTPA PC cell lines using microcell-mediated chromosome transfer. PK9 microcell hybrids with an introduced human chromosome 3 showed significant morphological changes and rapid growth arrest. Intriguingly, microcell hybrid clones of LTPA cells with an introduced human chromosome 3 (LTPA#3) showed suppression of mTert transcription, cell proliferation, and invasion compared with LTPA#4 cells containing human chromosome 4 and parental LTPA cells. Additionally, the promoter activity of mTert was downregulated in LTPA#3. Furthermore, we confirmed that TERT regulatory gene(s) are present in the 3p21.3 region by transfer of truncated chromosomes at arbitrary regions. These results provide important information on the functional significance of the LOH at 3p for development and progression of PC.
TRPV4 is a Prognostic Biomarker That Correlates With the Immunosuppressive Microenvironment and Chemoresistance of Anti-Cancer Drugs
Authors: wang kai , Feng Jun xing , Zheng Zhi ling , Chai Ying ze , Yu Hui jun , You Xin xin , Li Dan xiao , Cheng Dong xiao 
Published: April 2021

Abstract
Background: Transient receptor potential cation channel subfamily V member 4 (TRPV4) has been reported to regulate tumor progression in many tumor types. However, its association with the tumor immune microenvironment remains unclear.Methods: TRPV4 expression was assessed using data from The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) database. The clinical features and prognostic roles of TRPV4 were assessed using TCGA cohort. Gene set enrichment analysis (GSEA) of TRPV4 was conducted using the R package clusterProfiler. We analyzed the association between TRPV4 and immune cell infiltration scores of TCGA samples downloaded from published articles and the TIMER2 database.Results: TRPV4 was highly expressed and associated with worse overall survival (OS), disease-specific survival (DSS), disease-free interval (DFI), and progression-free interval (PFI) in colon adenocarcinoma (COAD) and ovarian cancer. Furthermore, TRPV4 expression was closely associated with immune regulation-related pathways. Moreover, tumor-associated macrophage (TAM) infiltration levels were positively correlated with TRPV4 expression in TCGA pan-cancer samples. Immunosuppressive genes such as PD-L1, PD-1, CTLA4, LAG3, TIGIT, TGFB1, and TGFBR1 were positively correlated with TRPV4 expression in most tumors.Conclusions: Our results suggest that TRPV4 is an oncogene and a prognostic marker in COAD and ovarian cancer. High TRPV4 expression is associated with tumor immunosuppressive status and may contribute to TAM infiltration based on TCGA data from pan-cancer samples.
Offset of Openings in Optic Nerve Head Canal at Level of Bruch’s Membrane, Anterior Sclera, and Lamina Cribrosa
Authors: Lee Kyoung Min , Ahn Hyoung Jun , Kim Martha , Oh Sohee , Kim Seok Hwan 
Published: April 2021

Abstract
We compared the central retinal vascular trunk (CRVT) position, as a surrogate of lamina cribrosa (LC) offset, with the anterior scleral opening (ASCO) offset from the Bruch’s membrane opening (BMO). Based on the BMO-centered radial scans, the BMO and ASCO margins were demarcated, and each center was determined as the center of the best-fitted ellipse for each margin. The ASCO/BMO offset was defined as the offset between each center. Angular deviations and the extent of ASCO and CRVT offsets from the BMO center were compared directly. Incomplete demarcation of ASCO was found in 20%, which was associated with a larger BMO area and a larger ASCO offset from the BMO. The angular deviation of ASCO offset was associated with that of CRVT offset and that of the longest externally oblique border. The ASCO offset was smaller than the CRVT offset, and, unlike the CRVT offset, it was rarely deviated to the inferior side. The complete ASCO margin might not be demarcateable when determined on BMO-centered radial scans in the presence of an offset. Also, the ASCO, which reflects only the superficial scleral layer, might not reflect the LC position, because the LC might be shifted further from the ASCO.