bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bortezomib suppresses self‐renewal and leukemogenesis of leukemia stem cell by NF‐ĸB‐dependent inhibition of CDK6 in MLL‐rearranged myeloid leukemia
Authors: Zhou Bin , Qin Yaqian , Zhou Jingying , Ruan Jichen , Xiong Fang , Dong Jinglai , Huang Xingzhou , Yu Zhijie , Gao Shenmeng 
Published: February 2021

AbstractAcute myeloid leukaemia (AML) with chromosomal rearrangements involving the H3K4 methyltransferase mixed‐lineage leukaemia (MLL) is an aggressive subtype with low overall survival. Bortezomib (Bort) is first applied in multiple myeloma. However, whether bort possesses anti‐self‐renewal and leukemogenesis of leukaemia stem cell (LSC) in AML with MLL rearrangements is still unclear. Here, we found that bort suppressed cell proliferation and decreased colony formation in human and murine leukaemic blasts. Besides, bort reduced the frequency and function of LSC, inhibited the progression, and extended the overall survival in MLL‐AF9 (MF9) ‐transformed leukaemic mice. Furthermore, bort decreased the percentage of human LSC (CD34+CD38‐) cells and extended the overall survival in AML blasts‐xenografted NOD/SCID‐IL2Rγ (NSG) mice. Mechanistically, cyclin dependent kinase 6 (CDK6) was identified as a bort target by RNA sequencing. Bort reduced the expressions of CDK6 by inhibiting NF ĸB recruitment to the promoter of CDK6, leading to the abolishment of NF ĸB DNA‐binding activity for CDK6 promoter. Overexpression of CDK6 partially rescued bort‐induced anti‐leukemogenesis. Most importantly, bort had little side‐effect against the normal haematological stem and progenitor cell (HSPC) and did not affect CDK6 expression in normal HSPC. In conclusion, our results suggest that bort selectively targets LSC in MLL rearrangements. Bort might be a prospective drug for AML patients bearing MLL rearrangements.
Impact of low‐dose gaseous ozone treatment to reduce the growth of in vitro broth cultures of foodborne pathogenic/spoilage bacteria in a food storage cold chamber
Authors: Bigi Francesco , Haghighi Hossein , Quartieri Andrea , De Leo Riccardo , Pulvirenti Andrea 
Published: February 2021

AbstractCold storage coupled with gaseous ozone represents a potential strategy to reduce or inhibit the presence of pathogenic and spoilage bacteria in a food storage cold chamber. This study aims to evaluate the impact of gaseous ozone treatment (0.05 ppm at exposure times of 30 and 60 min) on the bacterial contamination of internal surface and air in a cold chamber (3°C) intended for food storage. The bacterial load of internal surfaces was reduced by 0.99 ± 0.24 and 1.35 ± 0.27 log after 30 and 60 min ozone treatment, respectively. Airborne bacterial load was reduced by 0.93 ± 0.24 log after 30 min ozone treatment and became non‐detectable after 60 min. Gaseous ozone treatments (0.05 ppm at exposure times of 1, 2, 6, 24, 30, and 48 hr) of the cold chamber were investigated to evaluate the effectiveness of this technology against Escherichia coli, Listeria monocytogenes, Salmonella enterica Typhimurium, Campylobacter jejuni, and Pseudomonas fluorescens cultured in broth cultures. Ozone treatment was effective against C. jejuni since the population at the highest concentration of inoculum (3.34 log CFU/plate) was reduced by 2.23 log after 1 hr and it was completely undetectable after 2 hr. S. enterica and E. coli showed the highest resistance to short ozone treatment since 6 hr treatment did not show antibacterial activity whereas after 24 hr treatment around 2 log reduction was observed for both pathogens. Short ozone treatment did not affect L. monocytogenes viability. P. fluorescens showed high sensitivity to short treatments, with 0.75–1.32 log reductions after 1 hr and further 0.22–0.53 log reductions after 6 hr.
Dynamic changes in the pancreatitis activity scoring system during hospital course in a multicenter, prospective cohort
Authors: Paragomi Pedram , Tuft Marie , Pothoulakis Ioannis , Singh Vikesh K , Stevens Tyler , Nawaz Haq , Easler Jeffrey J , Thakkar Shyam , Cote Gregory A , Lee Peter J , Akshintala Venkata , Kamal Ayesha , Gougol Amir , Phillips Anna Evans , Machicado Jorge D , Whitcomb David C , Greer Phil J , Buxbaum James L , Hart Phil , Conwell Darwin , Tang Gong , Wu Bechien U , Papachristou Georgios I 
Published: February 2021

AbstractBackground and AimThe primary aim was to validate the Pancreatitis Activity Scoring System (PASS) in a multicenter prospectively ascertained acute pancreatitis (AP) cohort. Second, we investigated the association of early PASS trajectories with disease severity and length of hospital stay (LOS).MethodsData were prospectively collected through the APPRENTICE consortium (2015–2018). AP severity was categorized based on revised Atlanta classification. Delta PASS (ΔPASS) was calculated by subtracting activity score from baseline value. PASS trajectories were compared between severity subsets. Subsequently, the cohort was subdivided into three LOS subgroups as short (S‐LOS): 2–3 days; intermediate (I‐LOS): 3–7 days; and long (L‐LOS): ≥7 days. The generalized estimating equations model was implemented to compare PASS trajectories.ResultsThere were 434 subjects analyzed including 322 (74%) mild, 86 (20%) moderately severe, and 26 (6%) severe AP. Severe AP subjects had the highest activity levels and the slowest rate of decline in activity (P = 0.039). Focusing on mild AP, L‐LOS subjects (34%) had 28 points per day slower decline; whereas, S‐LOS group (13%) showed 34 points per day sharper decrease compared with I‐LOS (53%; P < 0.001). We noticed an outlier subset with a median admission‐PASS of 466 compared with 140 in the rest. Morphine equivalent dose constituted 80% of the total PASS in the outliers (median morphine equivalent dose score = 392), compared with only 25% in normal‐range subjects (score = 33, P value < 0.001).ConclusionsThis study highlighted that PASS can quantify AP activity. Significant differences in PASS trajectories were found both in revised Atlanta classification severity and LOS groups, which can be harnessed in AP monitoring/management (ClincialTrials.gov number, NCT03075618).
Age‐Related Trajectories of Cardiovascular Risk and Use of Aspirin and Statin Among U.S. Adults Aged 50 or Older, 2011–2018
Authors: Rhee Taeho Greg , Kumar Manish , Ross Joseph S. , Coll Patrick P. 
Published: February 2021

AbstractObjectivesTo examine age‐related trajectories of cardiovascular risk and use of aspirin and statin among U.S. adults aged 50 or older.DesignRepeated cross‐sectional study using data from 2011 to 2018 National Health and Nutrition Examination Surveys.SettingNationally representative health interview survey in the United States.ParticipantsNon‐institutionalized adults aged 50 years and older (n = 11,392 unweighted).MeasurementsPrimary prevention was defined as the prevention of a first cardiovascular event including coronary heart disease, angina/angina pectoris, heart attack, or stroke, whereas secondary prevention was defined as those with a history of these clinical conditions. Medication use was determined by self‐report; aspirin use included dose and frequency, and statin use included generic names, days of prescription fills, and indications. We examined linear trends between age and each medication use, after controlling for period, sex, and race/ethnicity.ResultsPrevalence of those eligible for primary prevention treatment increased with age from 31.8% in ages 50–54 to 52.0% in ages ≥75 (p < 0.001). Similarly, those eligible for secondary prevention treatment increased with age from 2.7% in ages 50–54 to 21.1% in ages ≥75 (p < 0.001). Low‐dose daily aspirin use increased with age (p < 0.001), and 45.3% of adults aged ≥75 took low‐dose aspirin daily for primary prevention. Statin use also increased with age (p < 0.001), and 56.4% of adults aged ≥75 had long‐term statin use for secondary prevention.ConclusionWhile adults aged ≥75 do not benefit from the use of aspirin to prevent the first CVD, many continue to take aspirin on a regular basis. In spite of the clear benefit of statin use to prevent a subsequent CVD event, many older adults in this risk category are not taking a statin. Further education and guidance for both healthcare providers and older adults regarding the appropriate use of aspirin and statins to prevent CVD is needed.
Individual mental health and couple functioning following couple relationship education participation: Exploring prospective cross‐lagged influences among changes
Authors: Cooper Erin , Adler‐Baeder Francesca , McGill Julianne 
Published: February 2021

AbstractLimited research on couple relationship education (CRE) programs explores the relationships among potential outcomes over time. This study conducted tests of processes of change in CRE participants’ mental health and couple functioning, based on previous evidence of concurrent benefit in these domains following CRE participation. Using a diverse sample of 926 men and women we first tested the stress generation model (Journal of Abnormal Psychology, 1991, 100(4), 555–561) assumptions that individual functioning influences relational functioning. We found support for immediate changes in mental health predicting changes in couple functioning 6 months later. However, a full cross‐lagged prospective model comparatively testing the stress generation model and the marital discord model (Depression in marriage: A model for etiology and treatment. Guilford, 1990), which emphasizes relational functioning impacts on individual well‐being over time, revealed the stronger directional link for both men and women was from immediate changes in couple functioning to later changes in individual mental health. Practical implications and future research directions are suggested.
Toward proficiency in telebehavioral health: applying interprofessional competencies in couple and family therapy
Authors: Hertlein Katherine M. , Drude Kenneth P. , Hilty Donald M. , Maheu Marlene M. 
Published: February 2021

AbstractDuring the coronavirus pandemic, many behavioral health professionals providing psychotherapeutic services, including couple/marriage and family therapists (C/MFTs), quickly changed from providing in‐person services to telebehavioral health (TBH) services, with specific reliance on teleconferencing. Many therapists were thrust into telehealth with minimal or no prior telebehavioral health experience, education, or training. Although TBH services have been shown to be effective and efficient with mental health and substance abuse problems, the teaching and learning of telebehavioral competencies have generally not been included in the formal education and training received by C/MFTs. This article presents an existing interprofessional telebehavioral health competencies framework not before published in C/MFT journals. This article will also demonstrate how those competencies are applicable to the education, training, and practice of telebehavioral health by C/MFTs. Implications for educational, service, and regulatory organizations are presented.
Orexins (hypocretins): The intersection between homeostatic and hedonic feeding
Authors: Muthmainah Muthmainah , Gogos Andrea , Sumithran Priya , Brown Robyn M. 
Published: February 2021

AbstractOrexins are hypothalamic neuropeptides originally discovered to play a role in the regulation of feeding behaviour. The broad connections of orexin neurons to mesocorticolimbic circuitry suggest they may play a role in mediating reward‐related behaviour beyond homeostatic feeding. Here, we review the role of orexin in a variety of eating‐related behaviour, with a focus on reward and motivation, and the neural circuits driving these effects. One emerging finding is the involvement of orexins in hedonic and appetitive behaviour towards palatable food, in addition to their role in homeostatic feeding. This review discusses the brain circuitry and possible mechanisms underlying the role of orexins in these behaviours. Overall, there is a marked bias in the literature towards studies involving male subjects. As such, future work needs to be done to involve female subjects. In summary, orexins play an important role in driving motivation for high salient rewards such as highly palatable food and may serve as the intersection between homeostatic and hedonic feeding.
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Sensory profile of protected designation of origin water buffalo ricotta cheese by different sensory methodologies
Authors: Miele Nicoletta A. , Puleo Sharon , Di Monaco Rossella , Cavella Silvana , Masi Paolo 
Published: February 2021

AbstractThe disciplinary protocol of protected designation of origin (PDO) water buffalo ricotta cheese allows to use both different ingredients and production technologies. This does not allow having a straight sensory profile. This study aimed to characterize the sensory profile of different PDO water buffalo ricotta cheeses and compare them with cow and sheep ricotta cheeses. To this purpose, a classical descriptive analysis (DA) and the modified version of flash profile (mFP) were used to draw the sensory profile of eight different ricotta cheeses. Simultaneously, a chemical–physical characterization was also performed. DA approach allowed discriminating the samples both for ingredient origin and homogenization process. On the other hand, mFP discriminated the samples mainly for ingredient origin. However, in both cases, different ingredient origins mainly affected flavor and odor attributes, meanwhile the technological process affected, as expected, the textural attributes. In particular, the water buffalo ricotta cheeses were described with intense global flavor and global taste, soft and spreadable, and very homogeneous in appearance. In conclusion, both the sensory methodologies identified the homogenized water buffalo ricotta cheeses as those with the most balanced sensory profile of than the other ricotta cheeses.Practical ApplicationDrawing the sensory profile of PDO water buffalo ricotta cheese may help to preserve the identity of this product, which is protected by a disciplinary protocol lacking in sensory attributes definition. The mFP provides useful information on the key sensory attributes of the PDO product, identifying the peculiarities of the water buffalo's whey. However, the DA allows highlighting all the variables affecting the sensory profile of the product, also related to the production process. The sensory profile obtained could help the Consortium of PDO water buffalo ricotta cheese to promote this product on the market.
Knowledge, attitudes and influencers of North American dog‐owners surrounding antimicrobials and antimicrobial stewardship
Authors: Stein M. R. , Evason M. D. , Stull J. W. , McClure J. T. , Weese J. S. 
Published: February 2021

ObjectivesTo quantify the individual influences of antimicrobial cost, method of administration and drug importance in human medicine on dog‐owner antimicrobial preference, and determine knowledge, attitudes and influencers of dog‐owners surrounding antimicrobials and antimicrobial stewardship.Materials and MethodsData were collected through an online survey targeting three dog‐owner participant groups. These consisted of individuals residing in: (1) Canada, (2) USA and (3) any country recruited through an educational social media site. USA and Canadian participants were financially compensated. Conjoint analysis was used to quantify the influence of antimicrobial cost, method of administration and drug importance in human medicine. Descriptive and analytical statistics were used for data evaluation.ResultsA total of 809 surveys were completed. Antimicrobial cost accounted for 47% of dog‐owner preferences, followed by method of administration (31%) and drug importance in human medicine (22%). All groups preferred lower cost drugs that were administered once by injection. Participants were more likely to prefer drugs considered “very important” in human medicine, except for the social media participants, who preferred drugs that were “not at all important.” Most respondents (86%) reported antimicrobial resistance as important in human medicine and 29% believed antimicrobial use in pets posed a risk for antimicrobial resistance in humans. Participants recruited through social media, and those in the highest education category, were significantly more likely to report antimicrobial use in pets as a risk to people.Clinical SignificanceCost was the most important factor in dog‐owner antimicrobial preferences. There is a need for dog‐owner antimicrobial stewardship education.
From Christ to Compassion: The Changing Language of Pastoral Care
Authors: Bernau John A. 
Published: February 2021

AbstractThe rise of neurology, psychology, and psychiatry over the last 100 years has challenged the clergy's historical monopoly on dealing with “personal problems” and mental well‐being. In this study, I document the changing language of pastoral care by analyzing over seventy years of academic articles in theJournal of Pastoral Care and Counseling(N= 4,054) using structural topic modeling. Ultimately, I reveal a linguistic shift from the universal to the particular as pastoral care professionals drop language of human nature and morality for that of individual narratives. I also find a decline of overtly religious language since the 1950s in favor of a more ecumenical language of spirituality, hope, and presence. Both of these trends take place alongside a push for “evidence‐based” pastoral care. Together, these linguistic shifts offer insight into a seventy‐year struggle to provide authentic religious care in a world of competing alternatives.
Estimating the genetic diversity of Pacific salmon and trout using multigene eDNA metabarcoding
Authors: Weitemier Kevin , Penaluna Brooke E. , Hauck Laura L. , Longway Lucas J. , Garcia Tiffany , Cronn Richard 
Published: February 2021

AbstractGenetic diversity underpins species conservation and management goals, and ultimately determines a species’ ability to adapt. Using freshwater environmental DNA (eDNA) samples, we examined mitochondrial genetic diversity using multigene metabarcode sequence data from four Oncorhynchus species across 16 sites in Oregon and northern California. Our multigene metabarcode panel included targets commonly used in population genetic NADH dehydrogenase 2 (ND2), phylogenetic cytochrome c oxidase subunit 1 (COI) and eDNA (12S ribosomal DNA) screening. The ND2 locus showed the greatest within‐species haplotype diversity for all species, followed by COI and then 12S rDNA for all species except Oncorhynchus kisutch. Sequences recovered for O. clarkii clarkii were either identical to, or one mutation different from, previously characterized haplotypes (95.3% and 4.5% of reads, respectively). The greatest diversity in O. c. clarkii was among coastal watersheds, and subsets of this diversity were shared with fish in inland watersheds. However, coastal streams and the Umpqua River watershed appear to harbour unique haplotypes. Sequences from O. mykiss revealed a disjunction between the Willamette watershed and southern watersheds suggesting divergent histories. We also identified similarities between populations in the northern Deschutes and southern Klamath watersheds, consistent with previously hypothesized connections between the two via inland basins. Oncorhynchus kisutch was only identified in coastal streams and the Klamath River watershed, with most diversity concentrated in the coastal Coquille watershed. Oncorhynchus tshawytscha was only observed at one site, but contained multiple haplotypes at each locus. The characterization of genetic diversity at multiple loci expands the knowledge gained from eDNA sampling and provides crucial information for conservation actions and genetic management.
A multi‐objective genetic algorithm strategy for robust optimal sensor placement
Authors: Civera Marco , Pecorelli Marica Leonarda , Ceravolo Rosario , Surace Cecilia , Zanotti Fragonara Luca 
Published: February 2021

AbstractThe performance of a monitoring system for civil buildings and infrastructures or mechanical systems depends mainly on the position of the deployed sensors. At the current state, this arrangement is chosen through optimal sensor placement (OSP) techniques that consider only the initial conditions of the structure. The effects of the potential damage are usually completely neglected during its design. Consequently, this sensor pattern is not guaranteed to remain optimal during the whole lifetime of the structure, especially for complex masonry buildings in high seismic hazard zones. In this paper, a novel approach based on multi‐objective optimization (MO) and genetic algorithms (GAs) is proposed for a damage scenario driven OSP strategy. The aim is to improve the robustness of the sensor configuration for damage detection after a potentially catastrophic event. The performance of this strategy is tested on the case study of the bell tower of the Santa Maria and San Giovenale Cathedral in Fossano (Italy) and compared to other classic OSP strategies and a standard GA approach with a single objective function.
Organochlorine exposure and health effects in stranded Steller sea lions (Eumetopias jubatus) and Pacific harbor seals (Phoca vitulina richardii) from Oregon and southern Washington coasts
Authors: Gundersen Deke , Josefchak Daniel , Duffield Deborah A. , D'Alessandro Dalin N. , Rice James M. 
Published: February 2021

AbstractOrganochlorine (OC) pesticides and polychlorinated biphenyls (PCBs) have been detected in a variety of marine mammal species at levels associated with adverse health effects. Little is known about OC levels and impacts on health in pinnipeds with different life histories. We determined the health and levels of 18 OC pesticides and 16 PCB congeners in blubber samples from 20 Steller sea lions and 39 Pacific harbor seals stranded from Oregon and Southern Washington. The most commonly detected OC at the highest concentration was p,p′‐ dichlorodiphenyldichloroethylene (DDE). PCBs were detected in all samples as well. Hypothesis testing indicated that diseased Steller sea lions (males and females combined) had higher contaminant concentrations than healthy Steller sea lions, and diseased Pacific harbor seals had higher concentrations of total OCs than healthy animals. Differences were also noted between diseased and healthy animals when looking at individual sexes of each species. Diseased Steller sea lions had higher mean contaminant levels than diseased harbor seals and healthy Steller sea lions had higher mean contaminant concentrations than healthy Pacific harbor seals. These results show that species differences exist in both contaminant loads and sensitivity to contaminants, which may be due to differences in life histories and physiology.