bluuebunny/crossref_metadata_2025_split huggingface dataset:
PD-1 Suppresses the Osteogenic and Odontogenic Differentiation of Stem Cells From Dental Apical Papilla via Targeting SHP2/NF-κB axis
Authors: Li Na , Li Zehan , Fu Lin , Yan Ming , Wang Yanqiu , Yu Jinhua , Wu Jintao 
Published: September 2021

Abstract
BackgroundStem cells from the apical papilla (SCAPs) are important for tooth root development and regeneration of root dentin. Here, we examined the expression of programmed cell death protein-1 (PD-1) in SCAPs and investigated the effect of PD-1 on odontogenic and osteogenic differentiation and the relationship between PD-1 and cell differentiation and SHP2/NF-κB signals.MethodsSCAPs were obtained culture in the related medium. The proliferation ability was evaluated by cell counting kit 8 and 5‐ethynyl‐20‐deoxyuridine (EdU) assay. Alkaline phosphatase (ALP) activity assay, ALP staining, western blot, real-time RT-PCR, Alizarin Red S staining, and immunofluorescence staining were performed to explore the osteo/odontogenic potential and the involvement of SHP2/NF-κB pathways. Besides, we transplanted SCAPs component into mouse calvaria defects to evaluate osteogenesis in vivo. ResultsWe found that human SCAPs expressed PD-1 for the first time. PD-1 knockdown enhanced the osteo/odontogenic differentiation of SCAPs by suppressing SHP2 pathway and activating NF-κB pathway. Overexpression of PD-1 inhibited the osteogenesis and odontogenesis of SCAPs via activation of SHP2 signal and inhibition of NF-κB pathway. ConclusionPD-1 activated SHP2 signal to block NF-κB signal and then played a vital role in osteo/odontogenic differentiation of SCAPs.
A 15 Year Review (2006-2020) of Patient-Reported Outcomes (PRO) in United States Oncology Product Labeling and Trends in Sponsor Size and Oncology Experience
Authors: Cooper Lisa D , Wo Emily , Lee Irene 
Published: September 2021

Abstract
BackgroundDespite wide use of patient reported outcomes (PRO) tools in clinical development, resulting data is rarely incorporated into the US label. This study reviewed oncology product labels approved by the Food and Drug Administration (FDA) between 2006 and 2020 to determine if the rate of PRO inclusion in labeling has meaningfully changed. Sponsors were assessed to identify demographic trends in achieving PRO label success. MethodsFDA-approved drugs were searched utilizing the Drugs@FDA database by month from January 2006 to December 2020 for novel drug and biologic approvals. Labels and product summary basis of approval (SBA) were reviewed for inclusion of PRO data. Sponsor size and experience were determined for each product in the year of initial approval. Results155 oncology products received initial approval between 2006-2020, of which only 7 contained PRO data in the label. More than half (53.5%) of products had PRO data described in the SBA. Over time, PRO information increasingly been included in the product marketing application. Sponsors utilizing PRO data tend to be experienced in oncology development and larger in size. ConclusionsThere has been no meaningful change in inclusion of PRO data in oncology product labeling over the past 15 years. Recent FDA guidance and initiatives may provide additional clarification to support appropriate PRO tools to support label inclusion as well as another forum for distributing PRO data publicly.
A Novel Rat Model for Cerebral Venous Sinus Thrombosis Matched Clinical Syndrome: Combination of Clinical Cases and Experimental Validation
Authors: Mu Shuwen , Lin Yinghong , Xu Yongjun , Wei Xianqing , Zeng Zihuan , Lin Kunzhe , Zhu Linghua , Liu Qinghong , Qi Xingfeng , Wei Liangfeng , Liang Shengxiang , Wang Shousen 
Published: September 2021

Abstract
BackgroundCerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical course. To investigate its pathophysiology, an animal model with a properly matched clinic is needed. MethodsThis study consisted of clinical and animal studies. In the clinical section, eligible patients with CVST and healthy controls’ detailed data were recorded between January 1, 2016 and May 31, 2021. For the animal section, a novel rat model of CVST was established by inserting a water-swellable rubber into the superior sagittal sinus and applying imaging, histopathological, serological and behavioral tests to investigate the corresponding pathophysiological changes. A total of 19 CVST patients and 25 healthy controls were enrolled, and the data demonstrated the typical characteristics of venous infarction with elevated intracranial pressure, vasogenic edema, and secondary hemorrhage. A total of 80 rats were randomly divided into two groups: sham group (n = 40) and model group (n = 40). ResultsThe imaging results showed consistent characteristics of brain injury and venous morphology in the animal models and CVST patients. Histological and serological findings further illustrated the predominance of vasogenic edema as pathological change, while behavioral tests revealed anxiety and depression in model rats. ConclusionsWe established a novel CVST rat model with reproducible surgical methods and stabilization of pathological changes, and compared it to retrospective CVST patient data, proving that the model could almost reproduce the clinical syndromes. This model provides reliable support for the study of CVST pathophysiology and the evaluation of therapies.
Efficient and mild synthesis of pyrano-pyrimidines catalyzed by decorated multi-walled carbon nanotubes bearing cobalt, nickel and copper metals in water
Authors: Harouni Nasrin Saberi , Naeimi Hossein 
Published: September 2021

Abstract
Multicomponent reactions are reactions in which three or more are agreeable of raw interests, composed in a one-step chemical process and the product is formed they give. Since multivariate reactions are monovalent reactions and Single-step conversions to ideal synthesis are very close. In this research, one pot three components reaction was carried out between 1, 3-dimethylbarbituric acid, malononitrile and different aldehydes in the attendance of Cu/Co/Ni/MWCNTs as a recyclable catalyst. This catalyst indicated high catalytic actuality with good proficiency and reusable under mild reservation. This reaction is performed fine at ambient temperature. This method proposed numerous materials such as being environmentally amicable for short reaction times and creating high yield products. The catalysts were collected and specified with diversity spectroscopic, such as techniques, such as FT-IR, X-ray fracture, and scanning electron microscopy. After finalization of the reaction, the vintage was obsolete, purified and identified by the melting points, infrared spectroscopy (FT-IR) and the magnetic resonance of the hydrogen nucleus (1H NMR) techniques.
Identification of Key genes associated with polycystic ovary syndrome (PCOS) and ovarian cancer using an integrated bioinformatics analysis
Authors: Zou Juan , Li Yukun , Liao Nianchun , Liu Jue , Zhang Qunfeng , Luo Min , Xiao Jiao , Chen Yanhua , Wang Mengjie , Chen Kexin , Zeng Juan , Mo Zhongcheng 
Published: September 2021

Abstract
Background

Accumulating evidence suggested a strong association between polycystic ovary syndrome (PCOS) and ovarian cancer (OC), but the potential molecular mechanism is still unclear. In this study, we identify unrecognized but significant genes correlated to PCOS and OC via bioinformatics.
Materials and methods

Multiple bioinformatic analysis, such as Differential expression analysis, Univariate Cox analysis, functional and pathway enrichment analysis, protein–protein interaction (PPI) network construction, survival analysis, and Immune infiltration analysis were utilized. We further evaluated the effect of OGN on FSHR expression via immunofluorescence.
Results

The TCGA-OC dataset, GSE140082 (for OC) and GSE34526 (for PCOS) dataset were downloaded. 12 genes, RNF144B, LPAR3, CRISPLD2, JCHAIN, OR7E14P, IL27RA, PTPRD, STAT1, NR4A1, OGN, GALNT6 and CXCL11, were recognized as signature genes. Drug sensitive analysis was showed that OGN might be a hub gene in the progression of PCOS and OC. Experimental analysis found OGN could increase the FSHR expression, indicating OGN could regulate the hormonal response in PCOS and OC. Furthermore, correlation analysis indicated that the function of OGN might be closely related with m6A and ferroptosis.
Conclusions

Our study indicated 12 signatures that might involved in the prognosis significance of OC, and closely related the correlation between OC and PCOS. Furthermore, the hub gene, OGN, was a significant gene in the OC and PCOS progression via regulating the hormonal response.
Asymmetrical pythagorean-hodograph spline-based C4 continuous local corner smoothing method with jerk continuous feedrate scheduling along linear toolpath
Authors: Jiang Xin , Hu Yifei , Huo Guanying , Su Cheng , Wang Bolun , Li Hexiong , Shen Li-Yong , Zheng Zhiming 
Published: September 2021

Abstract
In computer numerical control systems, linear segments, which are generated by computer-aided manufacturing software, are the most widely used toolpath format. Since the linear toolpath is discontinuous at the junction of two adjacent segments, the fluctuations on velocity, acceleration and jerk are inevitable. Local corner smoothing is widely used to address this problem. However, most existing methods use symmetrical splines to smooth the corners. When any one of the linear segments at the corner is short, to avoid overlap, the inserted spline will be micro, thereby increasing the curvature extreme of the spline and reducing the feedrate along it. In this article, the corners are smoothed by a 𝐶4 continuous asymmetric Pythagorean-hodograph (PH) spline. The curvature extreme of the proposed spline is investigated first, and 𝐾=2.5 is determined as the threshold to constarin the asymmetry of the spline. Then a two-step strategy is used to generate a blended toolpath composed of asymmetric PH splines and linear segments. In the first step, the PH splines at the corners are generated under the premise that the transition lengths do not exceed half of the length of the linear segments. In the second step, the splines at the corners are re-planned to reduce the curvature extremes, if the transition error does not reach the given threshold and there are extra linear trajectories on both sides of the spline trajectory. Finally, the bilinear interpolation method is applied to determine the critical points of the smoothed toolpath, and a jerk-continuous feedrate scheduling scheme is presented to interpolate the smoothed toolpath. Simulations show that, under the condition of not affecting the machining quality, the proposed method can improve the machining efficiency by 7.84% to 23.98% compared to 𝐺3 and 𝐺4 methods.
Closed form solution in buckling optimization problem of twisted rod
Authors: Kobelev Vladimir 
Published: September 2021

Abstract
The applications of this method for stability problems are illustrated in this manuscript. In the context of twisted rods, the counterpart for Euler’s buckling problem is Greenhill's problem, which studies the forming of a loop in an elastic bar under torsion (Greenhill, 1883). We search the optimal shape of the rod along its axis. A priori form of the cross-section remains unknown. For the solution of the actual problem the stability equations take into account all possible convex, simply connected shapes of the cross-section. Thus, we drop the assumption about the equality of principle moments of inertia for the cross-section. The cross-sections are similar geometric figures related by a homothetic transformation with respect to a homothetic center on the axis of the rod and vary along its axis. The distribution of material along the length of a twisted rod is optimized so that the rod is of the constant volume T and will support the maximal moment without spatial buckling. The cross section that delivers the maximum or the minimum for the critical eigenvalue must be determined among all convex, simply connected domains. We demonstrate at the beginning the validity of static Euler’s approach for simply supported rod (hinged), twisted by the conservative moment. The applied method for integration of the optimization criteria delivers different length and volumes of the optimal twisted rods. Instead of the seeking for the twisted rods of the fixed length and volume, we directly compare the twisted rods with the different lengths and cross-sections using the invariant factors. The solution of optimization problem for twisted rod is stated in closed form in terms of the higher transcendental functions. In the torsion stability problem, the optimal shape of cross-section is the equilateral triangle.
Anthropocentrism in the Structure and Description of Language
Authors: Alanović Milivoj B. 
Published: September 2021

Although the connection between language, meaning and culture has been dealt with on several occasions in Serbianlinguistics, we thought it appropriate to point out several examples of the lexical-grammatical structure of the Serbian language that illustrate the anthropocentric view of relations in the world immediately surrounding man. In order to realise our intention, we opted for verb constructions in which we can see the semantic transition from the original to the secondary domains, where the original domain primarily indicates the state of affairs typical of man or living beings in general.
Estudo sobre ações sustentáveis para a organização de eventos em espaços livres de uso público
Authors: Cunha Souza Mariana Cristina , De Pádua Dennis 
Published: September 2021

Este artigo resulta de uma pesquisa de iniciação científica. O objetivo geral foi identificar os eventos realizados em espaços livres de uso público na cidade de Presidente Prudente/SP, demonstrando como podem ser estratégicos para o setor de Turismo e Eventos, possibilitando a incorporação de práticas e ações sustentáveis. A metodologia consistiu na revisão da literatura científica e pesquisa de campo auxiliada pela técnica da observação sistemática e entrevistas. Os resultados demonstram que, quanto aos impactos positivos com a utilização desses espaços para o eventos têm-se a valorização e possivelmente maiores investimentos financeiros nessas áreas; a efetiva apropriação dos espaços públicos, que reforçam e valorizam os elementos culturais e ambientais locais, melhorando a imagem da cidade e da gestão pública municipal; a utilização de elementos naturais da paisagem; maior mobilidade e participação de pessoas de diferentes grupos sociais, pois o acesso não é restrito. Os impactos negativos podem ser a degradação ambiental, com o descarte dos resíduos sólidos no chão, poluindo o ambiente e a paisagem; impactos sobre a vegetação existente na área, entre outros. A partir disso, considera-se que os espaços livres de uso público têm potencial para serem aproveitados, especialmente, como locais estratégicos na promoção de eventos com base nos princípios da sustentabilidade. Sugere-se aos organizadores de eventos, que no processo de planejamento, sejam pensadas práticas de sensibilização dos envolvidos, desde os organizadores até o público participante, ressaltando a importância da responsabilidade ambiental, a aplicação das ações sustentáveis e a utilização desses espaços para a realização de eventos na cidade.
Palavras-chave: Turismo. Eventos. Sustentabilidade. Espaço Público. Presidente Prudente.
Breast Hemangioma: Unique Presentation in a Patient with Klippel-
Trenaunay-syndrome
Authors: Siddiqui Ayesha Shamim , Zafar Ibtesam , Majeed Ayesha Isani , Riaz Ramish 
Published: September 2021

Background:
Klippel-Trénaunay-Syndrome (KTS) is characterized by a triad of varicose
veins, port-wine stain and soft tissue or bony hypertrophy, and the diagnosis of KTS can be
made if any two of these three features are present. Hemangiomas in various locations, e.g., skull,
brain, epidural and vertebral hemangioma, mediastinal, colonic hemangioma, intraneural/intramuscular
hemangiomas, are reported with KTS.

Case Presentation:
Benign vascular tumors may rarely develop malignant transformation as Bugarin-
Estrada et al. reported breast angiosarcoma in a patient diagnosed as Klippel-Trenaunay-Syndrome.
We reported a case of a 40-year-old female with a known case of Klipple-Trenaunay-Syndrome
with left leg varicosities, cutaneous nevus, as well as unfortunate development of deep venous
thrombosis and markedly enlarged right breast hemangioma. Due to low incidence or lack of
early detection of breast hemangioma, its diagnosis is challenging.

Conclusion:
The history of the patient and multi-modality imaging utilization can help in early and
accurate diagnosis of diseases leading to better prognosis.
Program asuhan gizi olahraga (PAGO) atlet sepatu roda sebagai strategi memperbaiki profil status gizi, biokimia dan kualitas asupan
Authors: Dieny Fillah Fithra , Jauharany Firdananda Fikri , Rahadiyanti Ayu , Fitranti Deny Yudi , Tsani A. Fahmy Arif , Kurniawati Dewi Marfu’ah 
Published: September 2021

Penelitian bertujuan menganalisis pengaruh pemberian intervensi gizi berupa edukasi dan konseling gizi pada atlet sepatu roda remaja terhadap status gizi, status hidrasi, status biokimia, dan asupan gizi. Penelitian one group pre and post test design yang melibatkan 11 atlet di Klub Sepatu Roda Kairos Semarang. Variabel terikat adalah profil antropometri (berat badan, tinggi badan, fat mass, massa otot, body water), status hidrasi, status biokimia (kadar gula darah puasa, asam urat, kolesterol, hemoglobin), dan asupan makan. Uji Wilcoxon digunakan untuk menganalisis perbedaan profi antropometri, status hidrasi, status biokimia, dan asupan makan subjek antara sebelum dan sesudah intervensi. Terdapat perbedaan yang signifikan pada berat badan (p=0,029), fat mass (p=0,003), status hidrasi subjek sebelum latihan (p=0,026), kadar gula darah (p=0,005), kadar hemoglobin (p=0,007), asupan karbohidrat (p=0,029) dan asupan seng (p=0,049) sebelum dan setelah intervensi. Program Asuhan Gizi Olahraga (PAGO) pada atlet sepatu roda terbukti berdampak pada perbaikan beberapa profil antropometri, status biokimia, dan asupan makan. Program for inline skating athletes as strategies to improve the profile of nutritional status, biochemistry and quality of food intakeAbstract: This study aims to determine the effect of nutrition intervention in the form of nutrition education and counseling for adolescent inline skating athletes on nutritional status, hydration status, biochemical status, and nutritional intake. Methods: One group pre and post test design research on 11 athletes at the Kairos Wheeled Shoes Club Semarang. The dependent variables were anthropometric profiles (body weight, height, fat mass, muscle mass, body water), hydration status, biochemical status (fasting blood sugar levels, uric acid, cholesterol, hemoglobin), and food intake. The Wilcoxon test was used to analyze differences in anthropometric profile, hydration status, biochemical status, and food intake of subjects between before and after the intervention, Results: There was a significant difference in body weight (p = 0.029), fat mass (p = 0.003), hydration status subjects before exercise (p = 0.026), blood sugar levels (p = 0.005), hemoglobin levels (p = 0.007), carbohydrate intake (p = 0.029) and zinc intake (p = 0.049) before and after the intervention. Sports Nutrition Care Programme to inline skates athletes is proven to have an impact on anthropometric profile, biochemical status, and intake.
Pengembangan instrumen tes dribbling pada permainan futsal menggunakan teknologi mikrokontroler arduino
Authors: Tauba Randy Febri , HB Bafirman 
Published: September 2021

Penelitian ini bertujuan untuk membuat prototipe instrumen tes dribbling futsal menggunakan teknologi mikrokontroler arduino yang terhubung dengan aplikasi android. Penelitian ini merupakan penelitian dan pengembangan (Research and Development). Subjek penelitian adalah Pemain Futsal SMA N 1, 2 dan 9 Sijunjung, Kecamatan Sijunjung, Kabupaten Sijunjung, Sumatera Barat. Untuk Validasi alat oleh tiga orang ahli yaitu ahli Evaluasi dan Tes Pengukuran, Ahli Futsal, dan Ahli IT. Pengembangan instrumen tes dribbling futsal ini dilakukan dengan uji kelompok kecil dengan sampel 15 orang putra dan uji kelompok besar dengan sampel 45 orang putra. Metode yang digunakan adalah metode validitas ahli dengan penilaian menggunakan instrumen angket dan metode test dan retest untuk uji reliabilitas alat yang dianalisis dengan menggunakan rumus r korelasional. Hasil dari penelitian ini Hasil dari penelitian ini didapatkan nilai validitas sebesar 91,33%, kategori “Sangat Baik/layak” dan uji reliabilitas kelompok kecil sebesar 0.986, dengan kategori “Tinggi” dan kelompok besar sebesar 0.947, dengan kategori “Tinggi”. Kemudian didapatkan nilai praktisitas sebesar 94% dan nilai efektivitas sebesar 90%, maka disimpulkan bahwa instrumen tes dribbling futsal menggunakan teknologi mikrokontroler arduino efektif digunakan sebagai alat ukur untuk mengukur kecepatan dribbling futsal. Development of dribbling test instruments in futsal games using arduino microcontroller technology AbstractThis study aims to make a prototype of the futsal dribbling test instrument using Arduino microcontroller technology that is connected to an Android application. This research is a research and development (Research and Development). The research subjects were Futsal Players SMA N 1, 2 and 9 Sijunjung, Sijunjung District, Sijunjung Regency, West Sumatra. For tool validation by three experts, namely Evaluation and Measurement Tests, Futsal Experts, and IT Experts. The development of the futsal dribbling test instrument was carried out with a small group test with a sample of 15 men and a large group test with a sample of 45 men. The method used is the expert validity method with an assessment using a questionnaire instrument and the test and retest method to test the reliability of the tools analyzed using the correlation r formula. The results of this study obtained a validity value of 91.33%, the category "Very Good / feasible" and the small group reliability test was 0.986, with the "High" category and the large group was 0.947, with the "High" category. Then obtained a practical value of 94% and an effectiveness value of 90%. Thus, it can be concluded that the futsal dribbling test instrument using arduino microcontroller technology is good for use as a measuring tool to measure the speed of futsal dribbling.