bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the incidence of periprocedural myocardial infarction between percutaneous coronary intervention with versus without rotational atherectomy using propensity score-matching
Authors: Mizuno Yusuke , Sakakura Kenichi , Jinnouchi Hiroyuki , Taniguchi Yousuke , Tsukui Takunori , Yamamoto Kei , Seguchi Masaru , Wada Hiroshi , Fujita Hideo 
Published: May 2021

AbstractComplications such as slow flow are frequently observed in percutaneous coronary intervention (PCI) with rotational atherectomy (RA). However, it remains unclear whether the high incidence of slow flow results in the high incidence of periprocedural myocardial infarction (PMI), reflecting real myocardial damage. The aim of this study was to compare the incidence of PMI between PCI with versus without RA using propensity score-matching. We included 1350 elective PCI cases, which were divided into the RA group (n = 203) and the non-RA group (n = 1147). After propensity score matching, the matched RA group (n = 190) and the matched non-RA group (n = 190) were generated. The primary interest was to compare the incidence of PMI between the matched RA and non-RA groups. Before propensity score matching, the incidence of slow flow and PMI was greater in the RA group than in the non-RA group. After matching, the incidence of slow flow was still greater in the matched RA group than in the matched non-RA group (16.8% vs. 9.5%, p = 0.048). However, the incidence of PMI was similar between the matched RA and matched non-RA group (7.4% vs. 5.3%, p = 0.528, standardized difference: 0.086). In conclusion, although use of RA was associated with greater risk of slow flow, use of RA was not associated with PMI after a propensity score-matched analysis. The fact that RA did not increase the risk of myocardial damage in complex lesions would have an impact on revascularization strategy for severely calcified coronary lesions.
Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin
Authors: Lai Michele , Amato Rachele , La Rocca Veronica , Bilgin Mesut , Freer Giulia , Spezia Piergiorgio , Quaranta Paola , Piomelli Daniele , Pistello Mauro 
Published: May 2021

AbstractAcid ceramidase (AC) is a lysosomal hydrolase encoded by the ASAH1 gene, which cleaves ceramides into sphingosine and fatty acid. AC is expressed at high levels in most human melanoma cell lines and may confer resistance against chemotherapeutic agents. One such agent, doxorubicin, was shown to increase ceramide levels in melanoma cells. Ceramides contribute to the regulation of autophagy and apoptosis. Here we investigated the impact of AC ablation via CRISPR-Cas9 gene editing on the response of A375 melanoma cells to doxorubicin. We found that doxorubicin activates the autophagic response in wild-type A375 cells, which effectively resist apoptotic cell death. In striking contrast, doxorubicin fails to stimulate autophagy in A375 AC-null cells, which rapidly undergo apoptosis when exposed to the drug. The present work highlights changes that affect melanoma cells during incubation with doxorubicin, in A375 melanoma cells lacking AC. We found that the remarkable reduction in recovery rate after doxorubicin treatment is strictly associated with the impairment of autophagy, that forces the AC-inhibited cells into apoptotic path.
Skyrmion crystals in centrosymmetric itinerant magnets without horizontal mirror plane
Authors: Yambe Ryota , Hayami Satoru 
Published: May 2021

AbstractWe theoretically investigate a new stabilization mechanism of a skyrmion crystal (SkX) in centrosymmetric itinerant magnets with magnetic anisotropy. By considering a trigonal crystal system without the horizontal mirror plane, we derive an effective spin model with an anisotropic Ruderman–Kittel–Kasuya–Yosida (RKKY) interaction for a multi-band periodic Anderson model. We find that the anisotropic RKKY interaction gives rise to two distinct SkXs with different skyrmion numbers of one and two depending on a magnetic field. We also clarify that a phase arising from the multiple-Q spin density waves becomes a control parameter for a field-induced topological phase transition between the SkXs. The mechanism will be useful not only for understanding the SkXs, such as that in Gd$$_2$$

2

PdSi$$_3$$

3

, but also for exploring further skyrmion-hosting materials in trigonal itinerant magnets.
RNA interference of an orthologue of Dicer of Meloidogyne incognita alludes to the gene’s importance in nematode development
Authors: Iqbal Sadia , Jones Michael G. K. , Fosu-Nyarko John 
Published: May 2021

AbstractDicers and dicer-like enzymes play an essential role in small RNA processing in eukaryotes. Nematodes are thought to encode one dicer, DCR-1; only that for Caenorhabditis spp. is well-characterised. Using genomic sequences of eight root-knot nematodes (Meloidogyne spp.), we identified putative coding sequences typical of eukaryotic DICERS. We noted that the primary and secondary structures of DICERS they encode were different for different Meloidogyne species and even for isolates of the same species, suggesting paralogy for the gene. One of the genes for M. incognita (Midcr-1.1) expressed in eggs, juvenile stage 2 and adults, with the highest expression in the adult females. All the Meloidogyne DICERS had seven major domains typical of those for Caenorhabditis spp. and humans with very similar protein folding. RNAi of Midcr-1.1 in J2s using seven dsRNAs, each based on sequences encoding the domains, induced mild paralysis but measurable knockdown was detected in J2s treated with five of the dsRNAs. For four of the dsRNAs, the RNAi effect lasted and reduced the nematode’s infectivity. Also, host plant delivery of dsRNAs complementary to coding sequences of the Dicer Dimerisation domain impaired development, reducing nematode infection by 71%. These results confirm the importance of the gene to nematode health.
Toward understanding COVID-19 pneumonia: a deep-learning-based approach for severity analysis and monitoring the disease
Authors: Zandehshahvar Mohammadreza , van Assen Marly , Maleki Hossein , Kiarashi Yashar , De Cecco Carlo N. , Adibi Ali 
Published: May 2021

AbstractWe report a new approach using artificial intelligence (AI) to study and classify the severity of COVID-19 using 1208 chest X-rays (CXRs) of 396 COVID-19 patients obtained through the course of the disease at Emory Healthcare affiliated hospitals (Atlanta, GA, USA). Using a two-stage transfer learning technique to train a convolutional neural network (CNN), we show that the algorithm is able to classify four classes of disease severity (normal, mild, moderate, and severe) with the average Area Under the Curve (AUC) of 0.93. In addition, we show that the outputs of different layers of the CNN under dominant filters provide valuable insight about the subtle patterns in the CXRs, which can improve the accuracy in the reading of CXRs by a radiologist. Finally, we show that our approach can be used for studying the disease progression in a single patient and its influencing factors. The results suggest that our technique can form the foundation of a more concrete clinical model to predict the evolution of COVID-19 severity and the efficacy of different treatments for each patient through using CXRs and clinical data in the early stages of the disease. This use of AI to assess the severity and possibly predicting the future stages of the disease early on, will be essential in dealing with the upcoming waves of COVID-19 and optimizing resource allocation and treatment.
Evidence for the importance of land use, site characteristics and vegetation composition for rooting in European Alps
Authors: Tasser Erich , Gamper Sonja , Walde Janette , Obojes Nikolaus , Tappeiner Ulrike 
Published: May 2021

AbstractPlant rooting strongly affects most hydrological, biogeochemical and ecological processes in terrestrial ecosystems, as it presents the main pathway for carbon, water and nutrient transfer from soil to the atmosphere and is a key factor in stabilizing the soil layer. Few studies have actually investigated the link between phytosociological and structural vegetation composition and diversity in soil rooting parameters. Our study provides a comprehensive evaluation of plant cover and diversity effects on rooting parameters dependent on different land-use types along a north–south transect in the Eastern Alps. We conducted field studies of root biomass, rooting density and rooting depth for the six main land-use types: intensively and lightly used hay meadows, pastures, arable land, agriculturally unused grasslands and forests. The variation in rooting parameters was explained by different aspects of species and functional richness, species and functional composition, functional traits, abundance of key species and site variables depending on the land-use types. Our results showed that different characteristics of biodiversity explained the variance in root parameters (mass, density and depth) to a high degree (determination coefficient R2 values varied between 0.621 and 0.891). All rooting parameters increased with increasing plant species richness, as well as with a higher diversity of plant functional traits. The inclusion of site parameters significantly increased the explained variance, while we could not find evidence for key species and their abundance to provide additional explanatory power. Allowing the effects to vary depending on land-use types turned out to be a necessity supporting the importance of considering land-use types for rooting. The findings indicate that vegetation composition has a clear relationship with rooting parameters across different habitats in the European Alps. As the effect of plant composition differs with respect to the land-use type, rooting can be monitored by land management to achieve the desired benefits. For example, intensified rooting through extensive management decreases erosion risk and increases carbon uptake.
Federal and state cooperation necessary but not sufficient for effective regional mental health systems: insights from systems modelling and simulation
Authors: Occhipinti Jo-An , Skinner Adam , Carter Samantha , Heath Jacinta , Lawson Kenny , McGill Katherine , McClure Rod , Hickie Ian B. 
Published: May 2021

AbstractFor more than a decade, suicide rates in Australia have shown no improvement despite significant investment in reforms to support regionally driven initiatives. Further recommended reforms by the Productivity Commission call for Federal and State and Territory Government funding for mental health to be pooled and new Regional Commissioning Authorities established to take responsibility for efficient and effective allocation of ‘taxpayer money.’ This study explores the sufficiency of this recommendation in preventing ongoing policy resistance. A system dynamics model of pathways between psychological distress, the mental health care system, suicidal behaviour and their drivers was developed, tested, and validated for a large, geographically diverse region of New South Wales; the Hunter New England and Central Coast Primary Health Network (PHN). Multi-objective optimisation was used to explore potential discordance in the best-performing programs and initiatives (simulated from 2021 to 2031) across mental health outcomes between the two state-governed Local Health Districts (LHDs) and the federally governed PHN. Impacts on suicide deaths, mental health-related emergency department presentations, and service disengagement were explored. A combination of family psychoeducation, post-attempt aftercare, and safety planning, and social connectedness programs minimises the number of suicides across the PHN and in the Hunter New England LHD (13.5% reduction; 95% interval, 12.3–14.9%), and performs well in the Central Coast LHD (14.8% reduction, 13.5–16.3%), suggesting that aligned strategic decision making between the PHN and LHDs would deliver substantial impacts on suicide. Results also highlighted a marked trade-off between minimising suicide deaths versus minimising service disengagement. This is explained in part by the additional demand placed on services of intensive suicide prevention programs leading to increases in service disengagement as wait times for specialist community based mental health services and dissatisfaction with quality of care increases. Competing priorities between the PHN and LHDs (each seeking to optimise the different outcomes they are responsible for) can undermine the optimal impact of investments for suicide prevention. Systems modelling provides essential regional decision analysis infrastructure to facilitate coordinated federal and state investments for optimal impacts.
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Authors: Klooster Jean Paul ten , Bol-Schoenmakers Marianne , van Summeren Kitty , van Vliet Arno L. W. , de Haan Cornelis A. M. , van Kuppeveld Frank J. M. , Verkoeijen Saertje , Pieters Raymond 
Published: May 2021

AbstractIL22 is an important cytokine involved in the intestinal defense mechanisms against microbiome. By using ileum-derived organoids, we show that the expression of anti-microbial peptides (AMPs) and anti-viral peptides (AVPs) can be induced by IL22. In addition, we identified a bacterial and a viral route, both leading to IL22 production by T cells, but via different pathways. Bacterial products, such as LPS, induce enterocyte-secreted SAA1, which triggers the secretion of IL6 in fibroblasts, and subsequently IL22 in T cells. This IL22 induction can then be enhanced by macrophage-derived TNFα in two ways: by enhancing the responsiveness of T cells to IL6 and by increasing the expression of IL6 by fibroblasts. Viral infections of intestinal cells induce IFNβ1 and subsequently IL7. IFNβ1 can induce the expression of IL6 in fibroblasts and the combined activity of IL6 and IL7 can then induce IL22 expression in T cells. We also show that IL22 reduces the expression of viral entry receptors (e.g. ACE2, TMPRSS2, DPP4, CD46 and TNFRSF14), increases the expression of anti-viral proteins (e.g. RSAD2, AOS, ISG20 and Mx1) and, consequently, reduces the viral infection of neighboring cells. Overall, our data indicates that IL22 contributes to the innate responses against both bacteria and viruses.
Ageing and population shrinking: implications for sustainability in the urban century
Authors: Jarzebski Marcin Pawel , Elmqvist Thomas , Gasparatos Alexandros , Fukushi Kensuke , Eckersten Sofia , Haase Dagmar , Goodness Julie , Khoshkar Sara , Saito Osamu , Takeuchi Kazuhiko , Theorell Töres , Dong Nannan , Kasuga Fumiko , Watanabe Ryugo , Sioen Giles Bruno , Yokohari Makoto , Pu Jian 
Published: May 2021

AbstractPopulation ageing and shrinking are demographic phenomena with far-reaching implications for sustainability in the current context of extensive and rapid urbanization. This Perspective rationalizes their interface by (a) identifying the challenges and opportunities that ageing and shrinking urban populations will have for implementing the sustainable development goals (SDGs), and (b) discussing some emerging interventions to capitalise on the opportunities and reduce the challenges to achieving sustainability. We argue that a diverse set of context-specific technological, socioeconomic, institutional and governance interventions would be needed to leverage effectively the opportunities and minimize the risks posed by ageing and shrinking urban populations for long-term sustainability.
Stroke Recurrence and Its Relationship With Language Abilities
Authors: Goldberg Emily B. , Meier Erin L. , Sheppard Shannon M. , Breining Bonnie L. , Hillis Argye E. 
Published: May 2021

Purpose
Many factors influence poststroke language recovery, yet little is known about the influence of previous stroke(s) on language after left hemisphere stroke. In this prospective longitudinal study, we investigated the role of prior stroke on language abilities following an acute left hemisphere ischemic stroke, while controlling for demographic and stroke-related factors, and examined if earlier stroke impacted language recovery at a chronic time point.

Method

Participants (
n
= 122) with acute left hemisphere ischemic stroke completed language evaluation and clinical neuroimaging. They were divided into two groups: single stroke (SS;
n
= 79) or recurrent stroke (RS;
n
= 43). A subset of participants (
n
= 31) completed chronic-stage re-evaluation. Factors studied included age, education, diabetes and hypertension diagnoses, lesion volume and broad location, group status, aphasia prevalence, and language scores.

Results
Groups did not differ in language performance across time points. The only significant group differences were that participants with RS were older, had smaller acute lesions, and were less educated. Stroke group membership (SS vs. RS) was not associated with language performance at either time point. In patients with prior stroke, large acute lesion volumes were associated with acute language performance, whereas both large acute and chronic volumes influenced recovery.

Conclusions
History of prior stroke in itself may not significantly influence language impairment after an additional acute left hemisphere stroke, unless it contributes substantially to the total volume of infarcted brain tissue. Chronic and acute lesion volumes should be accounted for in studies investigating poststroke language performance and recovery.

Supplemental Material

https://doi.org/10.23641/asha.14669715
Merging stellar and intermediate-mass black holes in dense clusters: implications for LIGO, LISA, and the next generation of gravitational wave detectors
Authors: Arca Sedda Manuel , Amaro Seoane Pau , Chen Xian 
Published: May 2021

Context.The next generation of gravitational wave (GW) observatories would enable the detection of intermediate-mass black holes (IMBHs), an elusive type of BH expected to reside in the centres of massive clusters, dwarf galaxies, and possibly the accretion discs of active galactic nuclei. Intermediate-mass ratio inspirals (IMRIs), which are composed of an IMBH and a compact stellar object, constitute one promising source of GWs detectable by this new generation of instruments.Aims.We study the formation and evolution of IMRIs triggered by interactions between two stellar BHs and an IMBH inhabiting the centre of a dense star cluster, with the aim of placing constraints on the formation rate and detectability of IMRIs.Methods.We exploit directN-body models varying the IMBH mass, the stellar BH mass spectrum, and the star cluster properties. Our simulations take into account the host cluster gravitational field and general relativistic effects via post-Newtonian terms up to order 2.5. These simulations are coupled with a semi-analytic procedure to characterise the evolution of the remnant IMBH after the IMRI phase.Results.Generally, the IMRI formation probability attains values of ∼5−50%, with larger values corresponding to larger IMBH masses. Merging IMRIs tend to map out the stellar BH mass spectrum, suggesting that IMRIs could be used to unravel the role of dynamics in shaping BH populations in star clusters harbouring an IMBH. After the IMRI phase, an initially almost maximal(almost non-rotating) IMBH tends to significantly decrease(increase) its spin. Under the assumption that IMBHs grow mostly via repeated IMRIs, we show that only sufficiently massive (Mseed > 300 M⊙) IMBH seeds can grow up toMIMBH > 103 M⊙in dense globular clusters (GCs). Assuming that these seeds form at a redshift ofz ∼ 2−6, we find that around 1−5% of them would reach typical masses of ∼500−1500 M⊙at redshiftz = 0 and would exhibit low spins, generallySIMBH < 0.2. Measuring the mass and spin of IMBHs involved in IMRIs could help to unravel their formation mechanism. We show that LISA can detect IMBHs in Milky Way GCs with a signal-to-noise ratioS/N = 10−100, or in the Large Magellanic Cloud, for which we get aS/N = 8−40. More generally, we provide the IMRI merger rate for different detectors, namely LIGO (ΓLIGO = 0.003−1.6 yr−1), LISA (ΓLISA = 0.02−60 yr−1), ET (ΓET = 1−600 yr−1), and DECIGO (ΓDECIGO = 6−3000 yr−1).Conclusions.Our simulations explore one possible channel for IMBH growth, namely via merging with stellar BHs in dense clusters. We find that the mass and spin of the IMRI components and the merger remnant encode crucial information about the mechanisms that regulate IMBH formation. Our analysis suggests that the future synergy among GW detectors will enable us to fully unravel IMBH formation and evolution.
Safety and efficacy of EUS-guided pancreatic duct drainage in symptomatic main pancreatic duct obstruction: Is there still a place for surgery?
Authors: Falque Arthur , Gasmi Mohamed , Barthet Marc , Gonzalez Jean-Michel 
Published: May 2021

Abstract
Background and study aims In patients with symptomatic dilation of the main pancreatic duct (MPD) for whom endoscopic retrograde cholangiopancreatography (ERCP) is impossible, surgery has long been the only available treatment. EUS-PD is described as a minimally invasive alternative for ductal decompression surgery. We describe the results of our experience with it.
Patients and methods This was a retrospective single-center study over 9 years. Twenty-seven patients, median age 61.8 years (range 36 to 85) who underwent EUS-PD for symptomatic MPD dilatation were included. The main objective was to evaluate the technical success (placement of a plastic stent between the stomach and the MPD). Secondary objectives were to document clinical success based on pain and quality of life (visual analogic scales and treatments) and complication rates, and to define a standardized management algorithm.
Results The technical success rate was 92.5 %. The rate of minor adverse events was 21 % (4 cases of non-specific postoperative pain and two cases of delayed benign edematous pancreatitis). The clinical success rate was 88 %, and half of patients in whom the procedure was successful had "complete regression" of pain and half "partial regression." Median follow-up was 34.2 months (range 4 to 108). During follow-up, 74 % of patients reported improvement in quality of life and no patients required secondary surgery.
Conclusion Provided it is performed in an expert center, EUS-PD is a minimally invasive, effective, and safe alternative to pancreatic surgical drainage in patients with symptomatic MPD dilatation with failure or in whom ERCP is impossible.