bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH DUKUNGAN SUAMI DAN KADER POSYANDU TERHADAP MINAT IBU MENGGUNAKAN KB IMPLAN
Authors: Widayati Agustina , Faraswati Riska , Hastri RR Retno 
Published: October 2021

Berdasarkan Profil Kesehatan Indonesia cakupan KB implant sebesar 11,20%. Proporsi KB aktif implan di Jawa Timur sebesar 10,23%. Data dari Dinas Kesehatan Kota Probolinggo tahun 2019, di Kelurahan Sumbertaman terdapat KB Aktifnya yaitu 1.470 PUS dan Implan (146 PUS) serta yang ber KB di RW 1 sebanyak 21 orang. Tujuan menganalisis Pengaruh Dukungan Suami Dan Kader Posyandu terhadap Minat Ibu Menggunakan KB Implan di RW 1 Kelurahan Sumbertaman Kota Probolinggo. Desain penelitian analitik korelasional dengan metode cross sectional. Penelitian dilaksanakan Bulan Mei - Juni di kelurahan Sumbertaman Kota Probolinggo dengan 107 sampel. Data dianalisis dengan uji Spearman dan Regresi Ordinal ?: 0,05. Hasil penelitian menunjukkan dukungan suami sangat mendukung sebesar 47 responden (43,9%), dukungan kader posyandu kurang mendukung sebesar 59 responden (55,1%), minat ibu dalam menggunakan alat kontrasepsi implant dengan kategori minat tinggi sebesar 57 responden (53,5%) dengan p value : 0,000. Kesimpulan ada Pengaruh Dukungan Suami Dan Kader Posyandu Terhadap Minat Ibu Menggunakan KB Implan. Saran bagi puskesmas/tenaga kesehatan meningkatkan pemberian edukasi secara berkala kepada suami dan keluarga tentang pentingnya menggunakan alat kontrasepsi dan membentuk kelompok peduli KB.

Kata kunci: Dukungan Suami, Dukungan Kader Posyandu, Minat Ibu, Alat Kontrasepsi Implan

ABSTRACT
Based on the Indonesian Health Profile, the coverage of implant family planning is 11.20%. The proportion of active implant family planning in East Java is 10.23%. Data from the Probolinggo Health Office in 2019, in Sumbertaman Village there were, namely 1,470 Childbearing Age Couple and implants (146 Childbearing Age Couple ) and 21 people who had an interest in implant Contraceptive in RW 1. The purpose was to analize the influence between husband support and Posyandu cadres to maternal interest in using implant family planning in RW 1, Sumbertaman, Probolinggo. The design of this research is correlational analytic using cross sectional method. The research was conducted on May - June in Sumbertaman, Probolinggo with 107 samples. Data collection analyzed by Spearman test and Ordinal Regression ?: 0.05. The results showed husband's support in the very supportive category was 47 respondents (43.9%), the support of posyandu cadres with less supportive criteria was 59 respondents (55.1%), the mother's interest in using implant contraceptives with high interest categories was 57 respondents (53,5%) with p value: 0,000. The conclusion of the study shows there is a influence between husband support and Posyandu cadres to maternal interest in using implant family planning. Suggestions are put forward for health centers / health workers to increase the provision of regular education to husbands and families about the importance of using contraceptives and to form family planning care groups or family planning villages.

Keywords: Husband's Support, Posyandu Cadre Support, Mother's Interest, Implant Contraceptives
PENINGKATAN DERAJAT KESEHATAN ANAK MELALUI PROGRAM PENYULUHAN KESEHATAN GIGI (CARA GOSOK GIGI YANG BENAR) DI PASIRSARI KOTA PEKALONGAN
Authors: Ni'matul Ulya  
Published: October 2021

Ketepatan menggosok gigi adalah hal terpenting pada perawatan gigi. Menyikat gigi dengan waktu dan cara yang benar sangatlah penting karena gigi dan mulut yang sehat mencerminkan kualitas hidup yang baik. Malas gosok gigi juga dapat menyebabkan penyakit ginggivitis, yaitu peradangan dan pembengkakan gusi yang dapat berakibat infeksi. Data Riskesdas tahun 2018 melaporkan bahwa proporsi masalah gigi dan mulut sebesar 57,6% dan yang mendapatkan pelayanan dari tenaga medis gigi sebesar 10,2%. Adapun proporsi perilaku menyikat gigi dengan benar sebesar 2,8%.. Tujuan dari kegiatan ini adalah memberikan pengetahuan dan mengajari anak-anak usia sekolah dasar cara menggosok gigi yang benar di lingkungan Kelurahan Pasirsari Kota Pekalongan. Pada kegiatan ini didapatkan hasil pengetahuan anak-anak sebelum diberikan penyuluhan sebagian besar berpengetahuan cukup sebessar 51,3% dan setelah diberikan penyuluhan mayoritas mempunyai pengetahuan baik (54%), dan 70,3% sudah bisa menggosok gigi dengan cara yang benar. Kegiatan ini diharapkan dapat berlanjut terus dan berkesinambungan di masyarakat, dan petugas kesehatan dapat dengan aktif melakukan pemeriksaan gigi selain di sekolah-sekolah.
PEMBERDAYAAN MANAJEMEN BENCANA GUNUNG RAUNG BERBASIS VIRTUAL REALITY PADA MASYARAKAT DESA JAMBEWANGI
Authors: Yanuar Akhmad , Oktamanicka Dayu Graceila , Mariam Eva , Belqis Melania , Marvel Shane Nathanael , Febria Nuartha Yehezkiel 
Published: October 2021

Indonesia merupakan negara yang mempunyai jumlah gunung aktif terbanyak di dunia Laporan mengenai peningkatan aktivitas diberikan sejak tanggal 21 Juni 2015. Satelit Landsat 8 National Aeronautics and Space Administration (NASA) mendeteksi adanya dua lubang magma sehingga diperkirakan tidak akan terjadi letusan besar. Material pijar mulai menyembur pada tanggal 26 Juni 2015 dan rangkaian letusan terjadi sejak tanggal 4 Juli 2015. Dalam hal ini masyrakat banyak tidak mengetahui cara penanganan apabila terjadi gunung meletus, sehingga perlu adanya solusi dalam menangani masalah kurang pengetahuan tentang hal tersebut. Masa pandemic menjadikan setiap pendidikan dan pelatihan harus dibatasi maka dari itu kegiatan pengabdian masyrakat ini menggunakan metode Daring dan Luring. Pada kegiatan daring (online) pemberian materi dilakukan melalui zoom lalu dilanjutkan dengan tanya jawab, kegiatan daring (online) ini berjalan dengan lancer dan mendaptkan antusias yang sangat baik dari masyarakat. Kegiatan berikutnya adalah kegiatan secara luring (offline) kegiatan ini berjalan lancar yang dihadiri oleh responden yang sangat antusias, bentuk kegiatannya adalah memberikan pelatihan menajemen bencana dengan menggunakan VR. Kesimpulan dari kegiatan pengabdian masyrakat ini adalah banyak masyrakat yang tidak tahu tentang penanganan manajemen bencana gunung merapi, setelah dilakukan pelatihan manajemen bencana menggunakan VR pengetahuan masyarakat tentang pengetahuan manajemen bencana meningkat, pelatihan menggunakan VR bisa menjadikan solusi dalam pemberian pelatihan di era pandemic covid 19.
Fabrication of g-C3N4 Nanosheets Anchored With Controllable CdS Nanoparticles for Enhanced Visible-Light Photocatalytic Performance
Authors: Wang Minggui , Wang Min , Peng Fang , Sun Xiaohuan , Han Jie 
Published: October 2021

Herein, g-C3N4/CdS hybrids with controllable CdS nanoparticles anchoring on g-C3N4 nanosheets were constructed. The effects of CdS nanoparticles on photocatalytic H2 production and organic molecule degradation for g-C3N4/CdS hybrids were investigated. The maximum rate of H2 production for g-C3N4/CdS sample was 1,070.9 μmol g−1 h−1, which was about four times higher than that of the individual g-C3N4 nanosheet sample. The enhanced photocatalytic performance for prepared hybrids could be mainly attributed to the following causes: the formed heterojunctions can contribute to the light absorption and separation of photogenerated electrons and holes, the two-dimensional layered structure facilitates the transmission and transfer of electrons, and high specific surface area could provide more exposed active sites.
CYP17A1–ATP2B1 SNPs and Gene–Gene and Gene–Environment Interactions on Essential Hypertension
Authors: Wei Bi-Liu , Yin Rui-Xing , Liu Chun-Xiao , Deng Guo-Xiong , Guan Yao-Zong , Zheng Peng-Fei 
Published: October 2021

Background: The association between the CYP17A1 and ATP2B1 SNPs and essential hypertension (referred to as hypertension) is far from being consistent. In addition to the heterogeneity of hypertension resulting in inconsistent results, gene–gene and gene–environment interactions may play a major role in the pathogenesis of hypertension rather than a single gene or environmental factor.Methods: A case–control study consisting of 1,652 individuals (hypertension, 816; control, 836) was conducted in Maonan ethnic minority of China. Genotyping of the four SNPs was performed by the next-generation sequencing technology.Results: The frequencies of minor alleles and genotypes of four SNPs were different between the two groups (p &lt; 0.001). According to genetic dominance model analysis, three (rs1004467, rs11191548, and rs17249754) SNPs and two haplotypes (CYP17A1 rs1004467G-rs11191548C and ATP2B1 rs1401982G-rs17249754A) were negatively correlated, whereas rs1401982 SNP and the other two haplotypes (CYP17A1 rs1004467A-rs11191548T and ATP2B1 rs1401982A-rs17249754G) were positively associated with hypertension risk (p ≤ 0.002 for all). Two best significant two-locus models were screened out by GMDR software involving SNP–environment (rs11191548 and BMI ≥ 24 kg/m2) and haplotype–environment (CYP17A1 rs1004467G-rs11191548C and BMI ≥ 24 kg/m2) interactions (p ≤ 0.01). The subjects carrying some genotypes increased the hypertension risk.Conclusions: Our outcomes implied that the rs1004467, rs11191548, and rs17249754 SNPs and CYP17A1 rs1004467G-rs11191548C and ATP2B1 rs1401982G-rs17249754A haplotypes have protective effects, whereas the rs1401982 SNP and CYP17A1 rs1004467A-rs11191548T and ATP2B1 rs1401982A-rs17249754G haplotypes showed adverse effect on the prevalence of hypertension. Several SNP–environment interactions were also detected.
Optimal Strategy for HIFU-Based Renal Sympathetic Denervation in Canines
Authors: Liao Qingyao , Yang Fang , Xiong Bo , Zheng Xiaoyu , Wang Yue , Qian Jun , Qiao Zhao , Huang Jing 
Published: October 2021

Background: The association between the treatment efficacy and safety of high-intensity focused ultrasound (HIFU)-based renal sympathetic denervation (RDN) and the acoustic energy dose applied has not been fully studied and may provide important understanding of the mechanism that led to failure of the WAVE IV trial. The objective of this study was to externally deliver different HIFU doses to canines for RDN treatment and to investigate the optimal energy dose for HIFU-based RDN.Methods: Thirty canines were divided into five RDN groups according to dose of acoustic energy applied, and a sham control group that consisted of four canines was used for comparisons. All animals in the RDN groups underwent the RDN procedure with different acoustic energy doses, while in the sham control group, renal arteries were harvested without being subjected to acoustic energy delivery and were imaged using color Doppler flow imaging (CDFI). Blood pressure (BP) was recorded, and blood samples were collected before the RDN procedure and at 28 days after the RDN procedure. Histological examinations and measurement of renal tissue norepinephrine concentration were performed in all retrieved samples.Results: Suppression of BP was significant in the 300 W (15.17/8.33 ± 1.47/1.21 mmHg), 250 W (14.67/9.33 ± 1.21/1.37 mmHg), and 200 W (13.17/9.17 ± 2.32/1.84 mmHg) groups. Semiquantitative histological assessment of periarterial nerves around the kidney revealed that target nerves in the 300 W (9.77 ± 0.63), 250 W (9.42 ± 0.67), and 200 W (9.58 ± 0.54) groups had the highest nerve injury scores, followed by the 150 W group (5.29 ± 0.62). Furthermore, decreased renal tissue norepinephrine concentration, together with decreased expression of tyrosine hydroxylase in the 300, 250, and 200 W groups demonstrated effective sympathetic depression following sufficient acoustic energy deposition. However, the renal artery injury score in the 300 W group (0.93 ± 0.13) was significantly higher than in the other groups (p &lt; 0.001).Conclusion: This study provides evidence that RDN effectiveness is based on the energy dose delivered and that 200–250 W is effective and safe in normal-sized canines.
Genome-Wide Assessment Characteristics of Genes Overlapping Copy Number Variation Regions in Duroc Purebred Population
Authors: Wang Zhipeng , Guo Yuanyuan , Liu Shengwei , Meng Qingli 
Published: October 2021

Copy number variations (CNVs) are important structural variations that can cause significant phenotypic diversity. Reliable CNVs mapping can be achieved by identification of CNVs from different genetic backgrounds. Investigations on the characteristics of overlapping between CNV regions (CNVRs) and protein-coding genes (CNV genes) or miRNAs (CNV-miRNAs) can reveal the potential mechanisms of their regulation. In this study, we used 50 K SNP arrays to detect CNVs in Duroc purebred pig. A total number of 211 CNVRs were detected with a total length of 118.48 Mb, accounting for 5.23% of the autosomal genome sequence. Of these CNVRs, 32 were gains, 175 losses, and four contained both types (loss and gain within the same region). The CNVRs we detected were non-randomly distributed in the swine genome and were significantly enriched in the segmental duplication and gene density region. Additionally, these CNVRs were overlapping with 1,096 protein-coding genes (CNV-genes), and 39 miRNAs (CNV-miRNAs), respectively. The CNV-genes were enriched in terms of dosage-sensitive gene list. The expression of the CNV genes was significantly higher than that of the non-CNV genes in the adult Duroc prostate. Of all detected CNV genes, 22.99% genes were tissue-specific (TSI &gt; 0.9). Strong negative selection had been underway in the CNV-genes as the ones that were located entirely within the loss CNVRs appeared to be evolving rapidly as determined by the median dN plus dS values. Non-CNV genes tended to be miRNA target than CNV-genes. Furthermore, CNV-miRNAs tended to target more genes compared to non-CNV-miRNAs, and a combination of two CNV-miRNAs preferentially synergistically regulated the same target genes. We also focused our efforts on examining CNV genes and CNV-miRNAs functions, which were also involved in the lipid metabolism, including DGAT1, DGAT2, MOGAT2, miR143, miR335, and miRLET7. Further molecular experiments and independent large studies are needed to confirm our findings.
21-Hydroxylase-Specific CD8+ T Cells in Autoimmune Addison’s Disease Are Restricted by HLA-A2 and HLA-C7 Molecules
Authors: Hellesen Alexander , Aslaksen Sigrid , Breivik Lars , Røyrvik Ellen Christine , Bruserud Øyvind , Edvardsen Kine , Brokstad Karl Albert , Wolff Anette Susanne Bøe , Husebye Eystein Sverre , Bratland Eirik 
Published: October 2021

ObjectivesCD8+ T cells targeting 21-hydroxylase (21OH) are presumed to play a central role in the destruction of adrenocortical cells in autoimmune Addison’s disease (AAD). Earlier reports have suggested two immunodominant CD8+ T cell epitopes within 21OH: LLNATIAEV (21OH342-350), restricted by HLA-A2, and EPLARLEL (21OH431-438), restricted by HLA-B8. We aimed to characterize polyclonal CD8+ T cell responses to the proposed epitopes in a larger patient cohort with AAD.MethodsRecombinant fluorescent HLA-peptide multimer reagents were used to quantify antigen-specific CD8+ T cells by flow cytometry. Interferon-gamma (IFNγ) Elispot and biochemical assays were used to functionally investigate the 21OH-specific T cells, and to map the exactly defined epitopes of 21OH.ResultsWe found a significantly higher frequency of HLA-A2 restricted LLNATIAEV-specific cells in patients with AAD than in controls. These cells could also be expanded in vitro in an antigen specific manner and displayed a robust antigen-specific IFNγ production. In contrast, only negligible frequencies of EPLARLEL-specific T cells were detected in both patients and controls with limited IFNγ response. However, significant IFNγ production was observed in response to a longer peptide encompassing EPLARLEL, 21OH430-447, suggesting alternative dominant epitopes. Accordingly, we discovered that the slightly offset ARLELFVVL (21OH434-442) peptide is a novel dominant epitope restricted by HLA-C7 and not by HLA-B8 as initially postulated.ConclusionWe have identified two dominant 21OH epitopes targeted by CD8+ T cells in AAD, restricted by HLA-A2 and HLA-C7, respectively. To our knowledge, this is the first HLA-C7 restricted epitope described for an autoimmune disease.
Analysis of Genotype-Phenotype Correlations in Patients With Degenerative Dementia Through the Whole Exome Sequencing
Authors: Sun Lin , Zhang Jianye , Su Ning , Zhang Shaowei , Yan Feng , Lin Xiang , Yu Jie , Li Wei , Li Xia , Xiao Shifu 
Published: October 2021

Background: Sporadic dementias generally occur in older age and are highly polygenic, which indicates some patients transmitted in a poly-genes hereditary fashion.Objective: Our study aimed to analyze the correlations of genetic features with clinical symptoms in patients with degenerative dementia.Methods: We recruited a group of 84 dementia patients and conducted the whole exome sequencing (WES). The data were analyzed focusing on 153 dementia-related causing and susceptible genes.Results: According to the American College of Medical Genetics and Genomics (ACMG) standards and guidelines, we identified four reported pathogenic variants, namely, PSEN1 c.A344G, APP c.G2149A, MAPT c.G1165A, and MAPT c.G742A, one reported likely pathogenic variant, namely, PSEN2 c.G100A, one novel pathogenic variants, SQSTM1 c.C671A, and three novel likely pathogenic variants, namely, ABCA7 c.C4690T, ATP13A2 c.3135delC, and NOS3 c.2897-2A &gt; G. 21 variants with uncertain significance in PSEN2, C9orf72, NOTCH3, ABCA7, ERBB4, GRN, MPO, SETX, SORL1, NEFH, ADCM10, and SORL1, etc., were also detected in patients with Alzheimer’s disease (AD) and frontotemporal dementia (FTD).Conclusion: The new variants in dementia-related genes indicated heterogeneity in pathogenesis and phenotype of degenerative dementia. WES could serve as an efficient diagnostic tool for detecting intractable dementia.
Inhibition of Rho Kinase by Fasudil Ameliorates Cognition Impairment in APP/PS1 Transgenic Mice via Modulation of Gut Microbiota and Metabolites
Authors: Yan Yuqing , Gao Ye , Fang Qingli , Zhang Nianping , Kumar Gajendra , Yan Hailong , Song Lijuan , Li Jiehui , Zhang Yuna , Sun Jingxian , Wang Jiawei , Zhao Linhu , Skaggs Keith , Zhang Han-Ting , Ma Cun-Gen 
Published: October 2021

Background:Fasudil, a Rho kinase inhibitor, exerts therapeutic effects in a mouse model of Alzheimer's disease (AD), a chronic neurodegenerative disease with progressive loss of memory. However, the mechanisms remain unclear. In addition, the gut microbiota and its metabolites have been implicated in AD.Methods:We examined the effect of fasudil on learning and memory using the Morris water-maze (MWM) test in APPswe/PSEN1dE9 transgenic (APP/PS1) mice (8 months old) treated (i.p.) with fasudil (25 mg/kg/day; ADF) or saline (ADNS) and in age- and gender-matched wild-type (WT) mice. Fecal metagenomics and metabolites were performed to identify novel biomarkers of AD and elucidate the mechanisms of fasudil induced beneficial effects in AD mice.Results:The MWM test showed significant improvement of spatial memory in APP/PS1 mice treated with fasudil as compared to ADNS. The metagenomic analysis revealed the abundance of the dominant phyla in all the three groups, includingBacteroidetes(23.7–44%) andFirmicutes(6.4–26.6%), and the increased relative abundance ratio ofFirmicutes/Bacteroidetesin ADNS (59.1%) compared to WT (31.7%). In contrast, theFirmicutes/Bacteroidetesratio was decreased to the WT level in ADF (32.8%). Lefse analysis of metagenomics identifieds_Prevotella_sp_CAG873as an ADF potential biomarker, whiles_Helicobacter_typhloniusands_Helicobacter_sp_MIT_03-1616as ADNS potential biomarkers. Metabolite analysis revealed the increment of various metabolites, including glutamate, hypoxanthine, thymine, hexanoyl-CoA, and leukotriene, which were relative to ADNS or ADF microbiota potential biomarkers and mainly involved in the metabolism of nucleotide, lipids and sugars, and the inflammatory pathway.Conclusions:Memory deficit in APP/PS1 mice was correlated with the gut microbiome and metabolite status. Fasudil reversed the abnormal gut microbiota and subsequently regulated the related metabolisms to normal in the AD mice. It is believed that fasudil can be a novel strategy for the treatment of AD via remodeling of the gut microbiota and metabolites. The novel results also provide valuable references for the use of gut microbiota and metabolites as diagnostic biomarkers and/or therapeutic targets in clinical studies of AD.
Networks Are Associated With Acupuncture Treatment in Patients With Diarrhea-Predominant Irritable Bowel Syndrome: A Resting-State Imaging Study
Authors: Zhao Tingting , Pei Lixia , Ning Houxu , Guo Jing , Song Yafang , Zhou Junling , Chen Lu , Sun Jianhua , Mi Zhongping 
Published: October 2021

Background: Irritable Bowel Syndrome (IBS), as a functional gastrointestinal disorder, is characterized by abdominal pain and distension. Recent studies have shown that acupuncture treatment improves symptoms of diarrhea-predominant irritable bowel syndrome (IBS-D) by altering networks in certain brain regions. However, few studies have used resting-state functional magnetic resonance imaging (fMRI) to compare altered resting-state inter-network functional connectivity in IBS-D patients before and after acupuncture treatment.Objective: To analyze altered resting-state inter-network functional connectivity in IBS-D patients before and after acupuncture treatment.Methods: A total of 74 patients with IBS-D and 31 healthy controls (HCs) were recruited for this study. fMRI examination was performed in patients with IBS-D before and after acupuncture treatment, but only at baseline in HCs. Data on the left frontoparietal network (LFPN), default mode network (DMN), salience network (SN), ventral attention network (VAN), auditory network (AN), visual network (VN), sensorimotor network (SMN), dorsal attention network (DAN), and right frontoparietal network (RFPN) were subjected to independent component analysis (ICA). The functional connectivity values of inter-network were explored.Results: Acupuncture decreased irritable bowel syndrome symptom severity score (IBS-SSS) and Hamilton Anxiety Scale (HAMA). It also ameliorated symptoms related to IBS-D. Notably, functional connectivity between AN and VAN, SMN and DMN, RFPN and VAN in IBS-D patients after acupuncture treatment was different from that in HCs. Furthermore, there were differences in functional connectivity between DMN and DAN, DAN and LFPN, DMN and VAN before and after acupuncture treatment. The inter-network changes in DMN-VAN were positively correlated with changes in HAMA, life influence degree, and IBS-SSS in IBS-D.Conclusion: Altered inter-network functional connectivity is involved in several important hubs in large-scale networks. These networks are altered by acupuncture stimulation in patients with IBS-D.