bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Long-run Dynamics of Financial Hedging, A New Determinants of Green Financing in E7 and G7 Countries
Authors: Xueying Wu , Sadiq Muhammad , Chien Fengsheng , Ngo Thanh Quang , Nguyen Anh-Tuan , Trinh The-Truyen 
Published: June 2021

Abstract
The study estimates the long-run dynamics of a cleaner environment in promoting the gross domestic product of E7 and G7 countries. The recent study intends to estimate the climate change mitigation factor for a cleaner environment with the GDP of E7 countries and G7 countries from 2010 to 2018. For long-run estimation, second-generation panel data techniques including Augmented Dickey-Fuller (ADF), Phillip-Peron technique and fully modified ordinary least square (FMOLS) techniques are applied to draw the long-run inference. The results of study are robust with VECM technique. The outcomes of study revealed that climate change mitigation indicators affect more to the GDP of G7 countries than E7 countries. The GDP of both E7 and G7 countries is found depleting due to less clean environment. However, green financing techniques may clean the environment and reinforce the confidence of policymakers on the elevation of green economic growth in G7 and E7 countries. Furthermore, results show that a 1% rise in green financing index improves the environmental quality by 0.375% in G-7 countries, while it purifies 0.3920% environment in E7 countries. There is a need to reduce environmental pollution, shift energy generation sources towards alternative, innovative and green sources.
Adsorption and Dehydration Reaction of Ethanol to Ethylene on Isomorphous B, Al, and Ga Substitution of H-ZSM-5 Zeolite: An Embedded ONIOM Study
Authors: Maeboonruan Nattida , Boekfa Bundet , Maihom Thana , Treesukol Piti , Kongpatpanich Kanokwan , Namuangruk Supawadee , Probst Michael , Limtrakul Jumras 
Published: June 2021

Abstract
Dehydration reactions are important in the petroleum and petrochemical industries, especially for the feedstock production. In this work, the catalytic activity of zeolites with different acidities for the dehydration of ethanol to ethylene was investigated by calculations on cluster models of three isomorphous B, Al, and Ga substitution of H-ZSM-5 zeolites. Detailed reaction profiles for the dehydration reaction, assuming either a stepwise or a concerted mechanism, were calculated by using the ONIOM(MP2:M06-2X) + SCREEP method. The adsorption energies of ethanol are -21.6, -28.1 and -27.7 kcal/mol on H-[B]-ZSM-5, H-[Al]-ZSM-5, H-[Ga]-ZSM-5 zeolites, respectively. The stepwise mechanism was preferred on all isomorphous zeolites. The activation energies for the ethoxy formation as the rate-determining step are in range of 40.0 to 42.3 kcal/mol. The results indicated that the order of catalytic activity were H-[Al]-ZSM-5 > H-[Ga]-ZSM-5 > H-[B]-ZSM-5 for catalyzing the dehydration of ethanol to ethylene. Besides the acid strength, the zeolite framework affected the reaction by stabilizing the reaction intermediates leading to more stable adsorption complexes and lower activation barriers.
SPP1 is a Prognostic Related Biomarker and Correlated With Tumor-Infiltrating Immune Cells in Ovarian Cancer
Authors: Gao Wen , Yin Sheng , Sun Haiyan , Shao Zhuyan , Shi Peipei , Liu Dongli , Zhu Tao 
Published: June 2021

Abstract
Background: Secreted phosphoprotein 1 (SPP1) plays a vital role in tumor progression of some cancer types, but little is known whether it is a bystander or an actual player on driving immune infiltration in ovarian cancer.Methods: In this study, the expression of SPP1 was identified by Oncomine, GEPIA and TIMER databases, and SPP1 immumohistochemical staining analysis was assessed by The HPA database. The clinical outcomes between SPP1 expression and ovarian cancer patients were evaluated via Kaplan-Meier Plotter and PrognoScan dataset. Immune infiltration analyses were explored using TIMER and TISIDB dataset. In addition, Functional enrichment analyses were performed with Metascape and GeneMANIA database.Results: SPP1 was found overexpressed in ovarian tumor tissues and high SPP1 expression was correlated with shorter OS and PFS survivals. Particularly, elevated SPP1 expression was significantly associated with stage III ovarian cancer. Notably, SPP1 expression was positively correlated with infiltrating levels of CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells. Furthermore, SPP1 expression showed strong correlations with diverse immune hallmark sets in ovarian cancer. Of particular importance, functional enrichment analysis suggested that SPP1 strong related with immune response.Conclusions: These findings imply that SPP1 is correlated with prognosis and immune cell infiltrating, offering a new potential immunotherapeutic target in ovarian cancer.Trial registration: Not applicable.
A Meta-heuristic Density-Based Subspace Clustering Algorithm for High Dimensional Data
Authors: Agarwal Parul , Mehta Shikha , Abraham Ajith 
Published: June 2021

Abstract
Subspace clustering is one of the efficient techniques for determining the clusters in different subsets of dimensions. Ideally, these techniques should find all possible non-redundant clusters in which the data point participates. Unfortunately, existing hard subspace clustering algorithms fail to satisfy this property. Additionally, with the increase in dimensions of data, classical subspace algorithms become inefficient. This work presents a new density-based subspace clustering algorithm (S_FAD) to overcome the drawbacks of classical algorithms. S_FAD is based on a bottom-up approach and finds subspace clusters of varied density using different parameters of the DBSCAN algorithm. The algorithm optimizes parameters of the DBCAN algorithm through a hybrid meta-heuristic algorithm and uses hashing concepts to discover all non-redundant subspace clusters. The efficacy of S_FAD is evaluated against various existing subspace clustering algorithms on artificial and real datasets in terms of F_Score and rand_index. Performance is assessed based on three parameters: average ranking, SRR ranking, and scalability on varied dimensions. Statistical analysis is performed through the Wilcoxon signed-rank test. Results reveal that S_FAD performs considerably better on the majority of the datasets and scales well up to 6400 dimensions on the actual dataset.
Comparison of 3-Factor Versus 4-Factor Prothrombin Complex Concentrate for Emergent Warfarin Reversal: A Systematic Review and Meta-Analysis
Authors: Margraf David , Brown Sarah J , Blue Heather L , Bezdicek Tamara L , Wolfson Julian , Chapman Scott A 
Published: June 2021

Abstract
Background: Patients requiring emergent warfarin reversal (EWR) have been prescribed three-factor prothrombin complex concentrate (PCC3) and four-factor prothrombin complex concentrate (PCC4) to reverse the anticoagulant effects of warfarin. There is no existing systematic review and meta-analysis of studies directly comparing PCC3 and PCC4. Methods: The primary objective of this systematic review and meta-analysis was to determine the effectiveness of achieving study defined target INR goal after PCC3 or PCC4 administration. Secondary objectives were to determine the difference in safety endpoints, thromboembolic events (TE), and survival during the patients’ hospital stay. Random-effects meta-analysis models were used to estimate the odds ratios (OR), and heterogeneity associated with the outcomes. The Newcastle-Ottawa Scale was used to assess study quality, and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed. Results: Ten full-text manuscripts and 5 abstracts provided data for the primary and secondary outcomes. Patients requiring emergent warfarin reversal had more than three times the odds of reversal to goal INR when they were given PCC4 compared to PCC3 (OR = 3.61, 95% CI: 1.97-6.60, p < 0.001). There was no meaningful clinical association or statistically significant result between PCC4 and PCC3 groups in TE (OR = 1.56, 95% CI: 0.83-2.91, p = 0.17), or survival during hospital stay (OR = 1.34, 95% CI: 0.81-2.23, p = 0.25). Conclusion: PCC4 is more effective than PCC3 in meeting specific predefined INR goals, and has similar safety profiles in patients requiring emergent reversal of the anticoagulant effects of warfarin.
Health-Related Quality of Life and Associated Factors Among Adult Patients With Heart Failure in Tertiary Hospitals, Tigray Region, Northern Ethiopia, 2020.
Authors: Shibeshy Abrhaley Belay , Tilahun Weyzer , Tsegabirhan Hagos , Haftu Mebrahtom , Belay Kiros 
Published: June 2021

Abstract
Background Heart failure is a major public health issue for many countries in Sub-Saharan African which causes enormous public health relevance resulting in significant disability, loss of economic productivity, and poor health-related quality of life. Although there is a dated improvement in heart failure management, the morbidity and mortality remain unacceptably high, and a lot of patients are affected by debilitating symptoms which adversely influence their quality of life. Therefore, the main aim of this study was to assess health-related quality of life and associated factors among adult patients with heart failure in tertiary hospitals, Tigray region, Ethiopia, 2020.Methods An institutional-based cross-sectional study was conducted from March 01-April 30, 2020. A simple random sampling method was used to enroll the 301 participants. Data were collected using a structured questionnaire-based interview. The health-related quality of life measuring tool was adapted from the medical outcomes study. Data was entered to and cleaned by Epi-Data manager version 4.4.2.1 and exported to a statistical package of social science version 22 for analysis. Binary logistic regression model (Adjusted Odds ratio, 95% Confidence Interval, and P-value < 0.05) was used to determine the factors that influence health-related quality of life.Results This study showed that 142(47.2%) patients had a poor health-related quality of life. The overall mean score of health-related quality of life was 61.7±20.5 among patients. Age above 60 years (AOR; 4.47, 95% CI; 1.87-10.68), no formal education (AOR; 3.45, 95% CI; 1.31-9.12), New-York Heart Association class-ɪᴠ (AOR; 6.50, 95% CI; 2.62-16.13) and the absence of social support (AOR; 2.52, 95% CI; 1.33-4.79) were significantly associated with poor health‐related quality of life.ConclusionsHealth-related quality of life among heart failure patients was poor for almost half of the participants. Patients with older age, no formal education, and higher New York Heart Association class need special attention in each follow-up evaluation. Furthermore, health professionals and governments should inform the benefit of social support to family members and friends who are involved in the care process among heart failure patients.
A Set of Reliable Samples for the Study of Biomarkers for the Early Diagnosis of Parkinson’s Disease
Authors: Konjevod Marcela , Sáiz Jorge , Barbas Coral , Bergareche Alberto , Ardanaz Eva , Huerta José Mª , Vinagre-Aragón Ana , Erro Mª Elena , Chirlaque Mª Dolores , Abilleira Eunate , Ibarluzea Jesús Mª , Amiano Pilar 
Published: June 2021

Abstract
Background: The identification of biomarkers for the early diagnosis of Parkinson’s Disease (PD) might improve treatment and avoid complications. However, the search of such biomarkers is a real challenge. The main reason for this is that finding samples for that purpose is not an easy task. In this work, we propose a set of reliable samples for the identification of biomarkers for the early diagnosis of PD. In fact, the original source of these samples can be searched for similar purposes when dealing with other diseases.Materials and methods: Case-control study included 12 plasma samples of subjects that subsequently developed PD and 21 plasma samples of matched healthy controls from the Spanish EPIC-Navarra cohort, part of the European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain study. All the case samples were provided by healthy volunteers who were followed up for a mean of 15.9 (± 4.1) years and developed PD disease later on. Therefore, these sample are ideal for the search of early biomarkers of PD. We used an analytical multiplatform based on liquid chromatography and tandem mass spectrometry in order to find significant differences in the amounts of previously reported metabolites that are altered when the disease has been established and diagnosed. Results: Out of all 40 analytes that were studied, seven significant metabolites were observed. Benzoic acid, palmitic acid, oleic acid, stearic acid, myo-inositol, sorbitol and quinolinic acid were significantly changed in subjects that developed PD. These metabolites are closely related to mitochondrial dysfunction, the oxidative stress and the mechanisms of energy production, which might indicate that these mechanisms are already affected before disease onset.Conclusions: We propose the samples from the EPIC study as reliable and priceless samples for the search of early biomarkers of PD, although many other diseases can be also studied as long as the information stored about the participants recorded such details. As a proof of concept, we showed that these samples were useful for the study of metabolites that may be altered before the participants developed PD. This might also be a starting point in the establishment of a well-founded panel of metabolites that can be used for the early detection of this disease.
CircTP63 Promotes Cell Proliferation and Invasion by Regulating EZH2 Via Sponging miR-217 in Gallbladder Cancer
Authors: Wang Shouhua , Tong Huanjun , Su Tingting , Zhou Di , Shi Weibin , Tang Zhaohui , Quan Zhiwei 
Published: June 2021

Abstract
Background: Gallbladder cancer (GBC) is the most common biliary tract malignancy and has a poor prognosis in patients with GBC. CircRNA TP63 (circTP63) has been implicated in some tumor proliferation and invasion in some tumors. The study aims to investigate the clinical significance and functional role of circTP63 in GBC.Methods: The expression of circTP63 in GBC was detected by qRT-PCR and the association between circTP63 expression and prognosis of GBC patients was analyzed. CCK8 assay, flow cytometry analysis, transwell assay and in vivo studies were used to evaluated the cell proliferation and invasion after circTP63 knockdown in GBC cells. Luciferase reporter assays and RNA pull-down assay were used to determine the correlation between circTP63 and miR-217. Besides, western blot analysis was also performed.Results: In the present study, we showed that circTP63 expression was upregulated in GBC tissues and cells. Higher circTP63 expression was associated with lymph node metastasis and short overall survival (OS) in patients with GBC. In vitro, knockdown of circTP63 inhibited cell proliferation, cell cycle progression, migration and invasion in GBC. Besides, we demonstrated that knockdown of circTP63 inhibited GBC cell EMT process. In vivo, knockdown of circTP63 inhibited tumor growth in GBC. Mechanistically, we demonstrated that circTP63 competitively bind to miR-217 and promoted EZH2 expression and finally facilitated tumor progression.Conclusions: Our findings demonstrated that circTP63 sponge miR-217 and regulated EZH2 expression and finally facilitates tumor progression. Thus, targeting circTP63 may be a therapeutic strategy for the treatment of GBC.
Pro-Inflammatory Cytokines and MicroRNAs in Male Infertility
Authors: Attia Hana , Finocchi Federica , Orciani Monia , Mehdi Meriem , J Ines Zidi , Lazzarini Raffaella , Balercia Giancarlo , Belmonte Monica Mattioli 
Published: June 2021

Abstract
Background: Male infertility is a problem that affects 10-15% of men of reproductive age. In particular, gametogenesis is a complex process in which inflammation may play a central role through the secretion of cytokines and the expression of microRNAs. We assessed the potential role of proinflammatory cytokines (TNF-α, IL-6 and IL-1α) and microRNAs (miR-146a-5p, miR-34a-5p and miR-23a-3p) in the seminal plasma of infertile men compared to controls furthermore, evaluating their correlation with seminal and biochemical parameters.Methods and Results: Expression of cytokines and microRNAs were analyzed by ELISA and q-PCR. Our data show that IL-1α was significantly increased in the azoospermic group compared to controls, TNF-α mRNA was more expressed in the oligozoospermic group than controls. There were no significant differences in miRNAs expression among the three groups. The correlations between sperm parameters and inflammatory markers were evaluated, however no significant difference was highlighted.Conclusions: The determination of each inflammatory marker separately in the seminal plasma of subfertile men, despite some significant differences, does not have a diagnostic value in male infertility even if an assay of selective pro-inflammatory cytokines and microRNAs in the semen may improve the diagnosis of male infertility.
The Remission of Type 2 Diabetes Mellitus in Chinese Patients After Metabolic Surgery and Its Preoperative Contributing Factors: A Cohort Study
Authors: Yang Yi , Niu Lijuan , Mu Liqian , Wu Runda , Yang Le , Tong Shan , Mao Zhongqi , Shi Bimin , Yin Jun 
Published: June 2021

Abstract
Background and Objective: Existing data about the contributing factors of operative effect for type 2 diabetes mellitus (T2DM) are still limited in Chinese patients, especially about preoperative blood routine and biochemical indexes. We evaluated a prospective cohort of T2DM patients in early and middle stage with obesity to assess the post-operative prognosis and investigate its contributing factors.Methods: Adult T2DM participants in early and middle stage with obesity were enrolled and received metabolic surgery. Clinical data such as age, sex, baseline body mass index, percentage excess weight loss, glycemia range and drug consumption were collected and analyzed. Patients were managed by a multi-disciplinary team and followed up for 12 months. Complete remission was defined as HbA1c < 6.0%, fasting glucose < 5.6 mmol/l, without pharmacological intervention for at least 3 months. Results: In this study, a total of 96 T2DM patients with metabolic surgery were included. Among them, 61 (63.54%) patients had complete remission and 85 (88.50%) had post-operative partial remission after a 12-month clinical follow-up. Only 1 patient was reported to have an anastomotic leak; no surgical mortality during the follow-up. According to the complete remission or not, the patients were divided into two groups. There were significant differences on stroke history, fasting blood sugar and white blood cell between them. Furthermore, in multivariable analyses models, preoperative triglyceride (adjusted OR, 0.585; 95%CI, 0.418-0.819; p<0.01) and preoperative lymphocyte count (adjusted OR, 2.647; 95%CI, 1.141-6.142; p < 0.05) was significantly associated with complete remission of T2DM.Conclusion: Our data suggest that T2DM patients with lower triglyceride and higher lymphocyte count tended to achieve post-operative remission. Preoperative evaluation of lipid metabolism and immune function may be helpful for the evaluation of prognosis of metabolic surgery.
The Use of the Artificial Neural Network for the Treatment Outcomes of Single-channel and Tri-channel Applicator Used in Cervical Cancer Based on High Dose Rate Brachytherapy
Authors: Hossain Alamgir , Miah Shahidul , Ray Prodip Kumer , Ghosh Ashim Kumar , Khatun Rawshan Ara , Sarker Julekha , Habib Shafayat , Sarker Shupti 
Published: June 2021

Abstract
The study aimed to evaluate the treatment outcomes of single-channel and tri-channel applicators for cervical cancer patients based on high dose rate brachytherapy using an artificial neural network. An artificial neural network (ANNs) model is proposed to predict the treatment outcomes for the single-channel applicator and tri-channel applicator in cervical cancer for high dose rate brachytherapy. Fifty-four patients of cervical cancer who were receiving external beam radiation therapy (EBRT) of 40-50 cGy, with chemotherapy, were selected in this study from 37 patients with cervical cancer being used to train and 17 for testing in this model. A model was developed for intracavitary brachytherapy to estimate the comparison of　treatment outcomes for the single-channel applicator and tri-channel applicators, demonstrating the sensitivity 100% and specificity 100 % and accuracy 100% for training and 87.5%, 77.8%, and 82.4% for testing, respectively, including AUC= 0.961. The survival rate was 85% and 95% for single-channel and tri-channel applicators at 2 years, respectively. A model approach for artificial neural networks based on gynecological brachytherapy is a promising method for patient's treatment, resulting in the dosimetry output of applicators; medical physicists can be decided the appropriate applicator for cervical cancer. The proposed model has the potential accuracy in judging the treatment outcomes for the single-channel applicator and tri-channel applicator in cervical cancer based on survival analysis.
Extracts of Sida Cordifolia Contain Polysaccharides Possessing Immunomodulatory Activity and Rosmarinic Acid Compounds With Antibacterial Activity
Authors: Iqbal Haroon , Wright Claire L , Jones Sue , Silva Goncalo Rosas da , McKillen John , Gilmore Brendan F , Kavanagh Owen , Green Brian 
Published: June 2021

Abstract
Background:The overuse of antibiotics has led to increased antimicrobial resistance, but plant-derived biological response modifiers represent a potential alternative to these drugs. This investigation examined the immunomodulatory and antibacterial activities of Sida cordifolia (used in ethnomedicinal systems to treat infectious disease). Methods:Successive extractions were performed from the roots of these plants in hexane, chloroform, methanol and water. Immunomodulatory activity was determined in a series of experiments measuring the responses of splenocytes, macrophages and an in vivo model of innate immunity (Galleria mellonella). Antibacterial activity was assessed by determining minimum inhibitory/bactericidal concentrations (MIC/MBCs) for various Gram-positive and Gram-negative bacterial strains. Results: Immunomodulatory activity was confined to the aqueous extract, and further fractionation and biochemical analysis yielded a highly potent polysaccharide-enriched fraction (SCAF5). SCAF5 is a complex mixture of different polysaccharides with multiple immunomodulatory effects including immune cell proliferation, antibody secretion, phagocytosis, nitric oxide production, and increased expression of pro-inflammatory cytokines. Furthermore, Galleria mellonella pre-treated with SCAF5 produced more haemocytes and were more resistant (P<0.001) to infection with methicillin-resistant Staphylococcus aureus (MRSA) with a 98% reduction in bacterial load in pre-treated larvae compared to the negative control. The antibacterial activity of Sida cordifolia was confined to the methanolic fraction. Extensive fractionation identified two compounds, rosmarinic acid and its 4-O-β-d-glucoside derivative, which had potent activity against Gram-positive antibiotic-resistant bacteria, including MRSA. Conclusions:Sida cordifolia counters bacterial infections through a dual mechanism, and immunomodulatory polysaccharides from this plant should be isolated and characterised to realise their potential as anti-infective agents. Such properties could be developed as an antibiotic alternative (1) in the clinic and (2) alternative growth promoter for the agri-food industry.