bluuebunny/crossref_metadata_2025_split huggingface dataset:
New Insights Into the Introgression Between Agropyron Cristatum P Genome and Wheat Genome
Authors: Zhang Zhi , Zhou Shenghui , Liu Weihua , Song Liqiang , Zhang Jinpeng , Han Haiming , Yang Xinming , Lin Yida , Li Xiuquan , Li Lihui 
Published: May 2021

Abstract
Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat and confers desirable agronomic traits to common wheat. A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races (CYR32 and CYR33). In this study, three disease resistance-related transcriptomes, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were searched from among A. cristatum full-length transcriptomes. Then, three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH)/genomic in situ hybridization (GISH) probes made from the DNA of three bacterial artificial chromosome (BAC) clones, three genes, and A. cristatum “Z559” were used to analyze the BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655. The results revealed the introgression can spontaneously occur between A. cristatum P genome and wheat genome, and indicated the three genes could constitute a gene cluster according to the positions of their FISH signals. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 wheat-A. cristatum breeding materials, which showed resistance to physiological races (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, our study not only provides new insights into the introgression between A. cristatum P genome and wheat genome, but also provides the desirable breeding materials for breeding practice.
Radiologic Criteria of Retropharyngeal Lymph Node Metastasis in Maxillary Sinus Cancer
Authors: Kosugi Yasuo , Suzuki Michimasa , Fujimaki Mitsuhisa , Ohba Shinichi , Matsumoto Fumihiko , Muramoto Yoichi , Kawamoto Terufumi , Oshima Masaki , Shikama Naoto , Sasai Keisuke 
Published: May 2021

Abstract
Objective: To determine the most appropriate radiologic criteria of metastatic retropharyngeal lymph nodes (RLNs) in patients with maxillary sinus cancer (MSC).Materials and Methods: We retrospectively evaluated 16 consecutive patients who underwent magnetic resonance imaging (MRI) before and after the treatment of locally advanced squamous cell carcinoma of the maxillary sinus. The minimal and maximal diameters of all RLNS were recorded. RLNs were classified as metastatic on the basis of the MRI follow-up (f/u). RLNs were considered non-metastatic if stable disease continued until the final MRI f/u and metastatic in cases with different evaluations (complete response, partial response, progressive disease) determined using Response Evaluation Criteria in Solid Tumours (RECIST) ver. 1.1. The receiver operating characteristic curve (ROC) and area under the curve (AUC) were used to assess the accuracy of various criteria in the diagnosis of metastatic RLNs. Results: Of the 34 RLNs in 16 cases observed on pretreatment MRI, 7 were classified as metastatic RLNs and 27 as non-metastatic RLNs. Using the radiologic criteria, metastatic RLNs tended to be diagnosed more accurately with the minimal axial diameter than with the maximal axial diameter (AUC; 0.97 vs. 0.73, p = 0.06). The most accurate size criterion of metastatic RLNs was a minimal axial diameter of 5 mm or larger, with an accuracy of 94.1% (32 of 34).Conclusions: The most appropriate radiologic criterion of metastatic RLNs in MSC is a minimal axial diameter of 5 mm or longer.
Novel 3D Structure of Silver Doped ZrO2 Combined Graphene-Based Mesopouros Silica Quaternary Nanocomposite for a Nonenzymatic Glucose Sensing Effects
Authors: Oh Won-Chun , Cho Kwang-Youn , Chanthai Saksit , Fatema Kamrun Nahar 
Published: May 2021

Abstract
We described the novel nanocomposite of silver doped ZrO2 combined graphene-based mesoporous silica (ZrO2-Ag-G-SiO2, ZAGS) in bases of low-cost and self-assembly strategy. Synthesized ZAGS were characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy, Nitrogen adsorption-desorption isotherms, X-ray photoelectron spectroscopy (XPS), and Diffuse Reflectance Spectroscopy (DRS). The ZAGS as a enzyme-free glucose sensor active material toward coordinate electro-oxidation of glucose was considered through cyclic voltammetry in significant organic electrolytes, such as phosphate-buffer (PBS) at pH 7.4 and commercial urine. Utilizing ZAGS, glucose detecting may well be finished with effective electrocatalytic performance toward organically important concentrations with the current reaction of 9.0 × 10-3 mAcm-2 and discovery restrain of 0.05 mmol/L at the lowest potential of +0.2 V, thus fulfilling the elemental prerequisites for glucose detecting within the urine. Likewise, ZAGS cathodes can be worked for glucose detecting within the nearness of interferometer substances (e.g., ascorbic corrosive, lactose and starch) in urine at proper pH condition. Our results highlight the potential usages for subjective and quantitative electrochemical investigation of glucose through the ZAGS sensor for glucose detecting stage permitted within the urine concentration.
LncRNA expression profile, functional analysis and potential biomarkers in children with pancreaticobiliary maljunction
Authors: Zhao Lian , Shi San-li , Guo Wan-liang 
Published: May 2021

Abstract
Background

The present study was aimed to investigate the expression profile of long non-coding RNAs (lncRNAs) and its potential as biomarker for pancreaticobiliary maljunction (PBM).
Methods

The differential expression of lncRNA and messenger RNA (mRNA) from 15 pediatric patients with PBM and 15 control subjects were analyzed with microArray and validated with quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Gene Ontology enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to investigate the biological functions of these genes. The area under curve (AUC) of receiver operating characteristic (ROC) curve was used to predict the biomarker of lncRNA in PBM.
Results

There were 2915 mRNAs and 173 lncRNAs upregulated, 2121 mRNAs and 316 lncRNAs downregulated in PBM cases, respectively. The enriched GO associated with differentially expressed mRNA were extracellular matrix, extracellular region and kinetochore. The most enriched pathway of Protein digestion and absorption was associated with cancer and PI3K-Akt signaling. Cis- and Trans-target gene analysis indicated that mRNAs were predicted to be regulated by one lncRNA and one mRNA corresponded to several lncRNAs. The results showed that the expression trends of NR_110876, NR_132344, XR_946886 and XR_002956345 were consistent with the microarray results, and the difference was statistically significant NR_132344, XR_946886 and XR_002956345 (P < 0.05).The AUC of ROC curve was significant only for XR_946886 (0.837, P < 0.001).
Conclusion

The present study indicated that lncRNAs were involved the pathogenesis of common bile duct in PBM and XR_946886 would be biomarkers for PMB.
COVID-19 Decision Impacts: Vaccine Hesitancy, its Barriers and Impact Studies: Taking Bayelsa State as an Example.
Authors: Olalekan Raimi Morufu , Lucky Emeka Chisom , Okoyen Ebikapaye , Clement Angalabiri , Ogbointuwei Christopher , Babatunde Atoyebi 
Published: May 2021

Abstract
Background: Immunization is the foundation of the primary health care system, an indisputable human right as well as a global health and development success story, saving millions of lives yearly. We now have vaccines to prevent more than 20 life-threatening diseases, helping people of all ages live longer, healthier lives. Yet despite tremendous progress, far too many people around the world, including nearly 20 million infants yearly have insufficient access to vaccines. In some countries, progress has stalled or even reversed, and there is a real risk that complacency will undermine past achievements.Purpose: The current study aimed to explores vaccine hesitancy, its barriers and impact studies regarding COVID-19 decision impacts and to provide policy and decision makers and operational staff with evidence to inform decisions to promote vaccine uptake across Bayelsa State. Methods: A literature review tried to do a deep dive by using a variety of search engines such as Scopus, Research Gate, Mendeley, Summon, PubMed, Google Scholar, Hinari, Dimension, Academia, CAB Abstract, OARE Abstract, SSRN search strategy to retrieve research publications, “grey literature” and expert working group reports, including authors field experience.Findings: Absence of uniform methods of organization in the various health care facilities upon which we were obliged to rely. Thus, affecting the overall immunization programme and health system. Hence, policy must urgently address these challenges with emphasis on policy clarity while continuously improving infrastructure.Conclusions: The findings suggest that the involvement of community-based leaders can improve community participation and acceptance, while enhancing and strengthening integrated disease surveillance and adverse events following immunization (AEFI) monitoring and reporting systems; and conducting integrated advocacy and communications activities to promote demand for vaccination as part of increasing overall demand and acceptability of all essential PHC services. Thus, breaking barriers of vaccine hesitancy.
miR-30e-5p Regulates Autophagy and Apoptosis by Targeting Beclin1 Involved in Contrast-induced Acute Kidney Injury
Authors: Liu Xiaoqin , Li Qingzhao , Sun Lixin , Chen Limei , Li Yue , Huang Beibei , Liu Yunshuang , Jiang Chunyang 
Published: May 2021

Aims:
This study aims to verify if miR-30e-5p targets Beclin1 (BECN1), a
key regulator of autophagy, and investigate the function of miR-30e-5p and Beclin1
through mediating autophagy and apoptosis in contrast-induced acute kidney injury (CIAKI).

Methods:
Human renal tubular epithelial HK-2 cells were treated with Urografin to construct
a cell model of CI-AKI. Real-time reverse transcription-polymerase chain reaction
was used to detect gene expression. The dual-luciferase reporting assay and endogenous
validation were used to verify targeting and regulating function. The expressions of protein
were detected using Western blot. Cell proliferation was detected using methylthiazolyldiphenyl-
tetrazolium bromide (MTT) assay. Cell apoptosis was detected using terminal-
deoxynucleoitidyl transferase mediated nick end labeling assay, and autophagy was
detected using transmission electron microscopy.

Results:
HK-2 cells exposed to Urografin for 2 h induced a significant increase in
miR-30e-5p. miR-30e-5p had a targeting effect on Beclin1. Moreover, Urografin exposure
can enhance cell apoptosis by increasing caspase 3 gene expression and inhibiting
autophagy, which was induced by decreased Beclin1 expression regulated by
miR-30e-5p, thereby resulting in renal cell injury. Downregulation of miR-30e-5p or upregulation
of Beclin1 restored cell vitality by promoting autophagy and suppressing apoptosis
in Urografin-treated cells.

Conclusions:
Urografin increased the expression of miR-30e-5p in HK-2 cells and thus
decreased Beclin1 levels to inhibit autophagy, but induced apoptosis, which may be the
mechanism for CI-AKI.
Photopharmacology of Azo-Combretastatin-A4: Utilizing Tubulin Polymerization Inhibitors and Green Chemistry as the Key Steps
Authors: Rastogi Shiva K. , Dunnigan Jennifer K. , Towne Adelyne C. , Zhao Zhenze , Du Liqin , Brittain William J. 
Published: May 2021

:
Tubulin Polymerization Inhibitors (TPIs) are promising ligands utilized in chemotherapy
for modern cancer treatment. However, the current TPIs exhibit many serious side
effects that may pose limitations in chemotherapy. Combretastatin A-4 (CA-4) is a natural
TPI that binds at the colchicine binding site located on microtubules. The only cis isomer of
CA-4 is bio-active; however, due to its short half-life, it isomerizes quickly to its bio-inactive
trans geometric isomer. For preventing shortcomings of CA-4, azobenzene based CA-4,
called azo-CA-4 (azo-CA-4) is identified as a novel TPI. The geometric isomerization of azo-
CA-4 can be controlled upon exposure to ultraviolet (UV) light to remotely control its bioactivity.
Cis-azo-CA-4 is 200-500 times more active (IC50 = 0.2-10 μM) than trans-azo-CA-4
(IC50 = 50-110 μM) against various cancer cell lines. Photo-pharmacology uses light to control
drug activity, introducing a unique mechanism to develop novel photo-responsive TPIs. Further, the green chemistry
approach using ethanol and water as a green solvent in the synthesis of azo-CA-4 delivers advanced methodology
in novel TPI development.
Intervention Use and Symptom Change With Unguided Internet-Based Cognitive Behavioral Therapy for Depression During the COVID-19 Pandemic: Log Data Analysis of a Convenience Sample
Authors: Oehler Caroline , Scholze Katharina , Reich Hanna , Sander Christian , Hegerl Ulrich 
Published: May 2021

Background
Internet- and mobile-based interventions are most efficacious in the treatment of depression when they involve some form of guidance, but providing guidance requires resources such as trained personnel, who might not always be available (eg, during lockdowns to contain the COVID-19 pandemic).

Objective
The current analysis focuses on changes in symptoms of depression in a guided sample of patients with depression who registered for an internet-based intervention, the iFightDepression tool, as well as the extent of intervention use, compared to an unguided sample. The objective is to further understand the effects of guidance and adherence on the intervention’s potential to induce symptom change.

Methods
Log data from two convenience samples in German routine care were used to assess symptom change after 6-9 weeks of intervention as well as minimal dose (finishing at least two workshops). A linear regression model with changes in Patient Health Questionnaire (PHQ-9) score as a dependent variable and guidance and minimal dose as well as their interaction as independent variables was specified.

Results
Data from 1423 people with symptoms of depression (n=940 unguided, 66.1%) were included in the current analysis. In the linear regression model predicting symptom change, a significant interaction of guidance and minimal dose revealed a specifically greater improvement for patients who received guidance and also worked with the intervention content (β=–1.75, t=–2.37, P=.02), while there was little difference in symptom change due to guidance in the group that did not use the intervention. In this model, the main effect of guidance was only marginally significant (β=–.53, t=–1.78, P=.08).

Conclusions
Guidance in internet-based interventions for depression is not only an important factor to facilitate adherence, but also seems to further improve results for patients adhering to the intervention compared to those who do the same but without guidance.
KERUGIAN KONTRAKTOR AKIBAT WASTE MATERIAL PROYEK KONSTRUKSI GEDUNG BERTINGKAT
Authors: Hendrik Sulistio  , Mega Waty  
Published: May 2021

Waste dalam bidang konstruksi dapat diartikan sebagai kehilangan atau kerugian berbagai sumber daya yaitu material, waktu (yang berkaitan dengan tenaga kerja dan peralatan) dan modal, yang di akibatkan oleh kegiatan-kegiatan yang membutuhkan biaya secara langsung maupun tidak langsung tetapi tidak menambah nilai kepada produk akhir bagi pihak pengguna jasa Penelitian ini dilakukan pada 64 responden proyek konstruksi gedung untuk mengetahui besarnya kerugian akibat beton ready mix dan penyebabnya. Penelitian dengan menggunakan kuesioner yang disebarkan melalui system online. Dari 64 responden menghasilkan 9.06% yang merupakan kerugian beton ready mix. Kerugian beton Ready Mix adalah 9.06 % dari profit Beton Ready Mix. Dari uji validitas dan uji reliabilitas menghasilkan 18 faktor penyebab yang menyebabkan kerugian waste material beton ready mix. Faktor Penyebab kerugian beton ready mix yang terbesar adalah: hilang karena dicuri, adanya perubahan desain, pendetailan gambar yang rumit dan pesanan material yang tidak sesuai dengan spesifikasi dan tercecer pada saat diangkut/dipindahkan.
ON CREATIVITY THROUGH MATHEMATIZATION IN SOLVING NON-ROUTINE PROBLEMS
Authors: Arifin Sujinal , Zulkardi Zulkardi , Putri Ratu Ilma Indra , Hartono Yusuf 
Published: May 2021

This study aimed to describe and compare the students’ fluency, flexibility, and originality in solving non-routine problems in the Palembang context. They were depicted from the student’s fluency, flexibility, and originality of solving the horizontal and vertical mathematization forms. This qualitative study employed. The subjects of this study were 30 students of grade nine of junior high schools in Palembang. The instruments used were tests and interviews. The tests were employed to investigate the written horizontal and vertical mathematizations forms. Meanwhile, the interviews were to explore the students’ ideas with inadequately detailed answers. Then, the test and interview data were reduced and grouped based on the indicators of creativity. The reduced data were presented in a descriptive form for conclusions. The results of the data analysis showed that the high-ability students were the most fluent and flexible in solving the problems. Still, the provided solutions were less original and tended to use formal mathematics in the forms of formulas, symbols, and operations. Meanwhile, the moderate-ability students tended to start to solve problems by simplifying them, then presenting them in visual images. The answer sheets of the moderate-ability students revealed their fluency in understanding the problems and solutions, flexibility, and originality of thinking. This study obtained different results from the low-ability students who tended to have difficulties understanding the problems and made many errors in solving them. Such a condition showed their inability to write the known data and relate the data to other facts they had already learned. As a result, their answers did not represent fluency, flexibility, and originality.
PEMBERDAYAAN MASYARAKAT MELALUI PELATIHAN KEWIRAUSAHAAN DAN PEMASARAN DIGITAL PADA BUMDes BLULUKAN GEMILANG
Authors: Nugrahaningsih Putri , Hanggana Sri , Murni Sri , Hananto Santoso Tri , Asrihapsari Andi , Syafiqurrahman M. , Zoraifi Renata , Hantoro Sri 
Published: May 2021

Salah satu BUMDes di Kabupaten Karanganyar adalah BUMDes Blulukan Gemilang yang merupakan lembaga ekonomi desa, tujuan pendirian BUMDes untuk menanggulangi kemiskinan yang mana unit usahanya mengembangkan jiwa kewirausahaan melalui unit usaha konfeksi dan unit pengelolaan sampah. Tujuan dari kegiatan pengabdian masyarakat ini adalah (1) meningkatkan pengetahuan wirausaha pengelola BUMDes; (2) memberikan pemahaman dan pendampingan tentang cara mempromosikan dan memasarkan produk secara digital. Oleh karena itu, perlu pengembangan kompetensi dan kewirausahaan sebagai fase lanjutan dari fase awal pendirian BUMDes. Metode kegiatan pengabdian masyarakat yang digunakan adalah partisipatif, yaitu pendekatan yang berorientasi kepada upaya peningkatan peran serta masyarakat secara langsung dalam berbagai proses dan pelaksanaan, dengan cara pendampingan dengan cara memberikan penyuluhan kepada staf pengelola BUMDes dan masyarakat (ibu ibu rumah tangga) tentang kewirausahaan dan manajemen pemasaran serta memberikan pendampingan dalam pemasaran dengan mempromosikan produk hasil unit konfeksi yang dikelola.Hasil pengabdian menunjukkan bahwa pelaksanaan pemberdayaan masyarakat yang dilaksanakan mengalami beberapa kendala dalam hal produksi dikarenakan adanya keterbatasan modal mesin produksi dan saluran pemasaran produk yang belum menggunakan media digital. Oleh karena itu, tim pengabdi memberikan bantuan modal aset tetap berupa mesin potong kain untuk memperbanyak produksi masker. Langkah selanjutnya, tim pengabdi melakukan pendampingan berupa kegiatan literasi tentang informasi digital melalui pelatihan digital marketing dengan media sosial dan desain produk masker di masa pandemi sebagai langkah memupuk jiwa kewirausahaan. Diharapkan setelah pelatihan, peserta siap beraksi menerapkan jiwa wirausaha. Hasil kegiatan pengabdian yang dilakukan pada BUMDes Blulukan Gemilang telah memberikan dampak pada peningkatan pendapatan masyarakat dan bertambahnya lapangan pekerjaan bagi masyarakat Desa.
Morphometric and meristic character variability and relationships among populations of Boops boops (L.) from four marine stations along the Tunisian coast
Authors: Ben Labidi Mouna , Allaya Hassen , Basyouny Shahin Adel A. , Quignard Jean-Pierre , Trabelsi Monia , Ben Faleh Abderraouf 
Published: May 2021

Abstract
The variability of 14 morphometric and seven meristic characters of Boops boops (L.) collected at four stations along the Tunisian coast was examined in 518 samples ranging from 126 to 206 mm in total length and 37 to 78 g in total weight. Statistical analysis of both sets of characters showed significant variation among the four populations in 13 (92.9%) morphometric and four (57.1%) meristic characters. CVA and cluster analysis of the morphometric characters revealed four groups corresponding to four populations, with 95.36% correct classifications of individuals to their populations of origin. However, CVA and cluster analysis of the meristic characters showed a reticulate relationship among the four populations, as there was clear differentiation between those in Monastir and Zarzis and an overlap between those in Bizerte and Kelibia, with only 51.75% of individuals correctly classified to their respective populations. This morphological differentiation among the populations from the four stations was attributed to the differences in the physico-chemical water properties at these stations. However, further studies are needed on the impact of environmental factors and diet at these stations on the morphological population structure of B. boops to better understand the contribution of environmental conditions to morphological variation.
The Common European Investment Policy and Its Perspectives in the Context of the Achmea Case Law
Authors: Nováčková Daniela , Peráček Tomáš 
Published: May 2021

Abstract
Current developments in the field of international investment relations are influenced by the ruling of the Court of Justice in the Achmea case, when de facto European law became superior to international law. The verdict of the Court of Justice changes the usual legal procedures and customs in the field of bilateral investment agreements. However, the impact of this court decision is an almost unexplored area due to the lack of interest of legal theorists, and it is relatively difficult to find answers to the ambiguities and problems that have arisen. The scientific study analyses the current process of introducing new rules in the field of investment policy within the European Union, which means the end of bilateral investment agreements within the European Union. It also examines the European Union’s activities in the field of foreign direct investment and the development of a stable European investment policy. Determining the goal of the scientific study is based directly on current needs and emerging practical problems in practice. Their correct understanding and application has a fundamental impact on the possibilities of rules in the field of investment policy. Due to the nature of the researched topic, we applied selected qualitative methods suitable for recognising the law. However, we also analysed scientific literature, case-law and the analogy of law, thus providing qualified answers to the application pitfalls of legal practice.