bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China
Authors: Wang Jupeng , Zhu Lina , Ma Mingkun , Chen Hui , Guo Suxiang , Wu Guangye , Li Shaohong , Wen Xuehong , Ren Li 
Published: September 2021

Abstract
Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.
A fetus with a mass in the oral cavity: a rare case of oral eruption cyst
Authors: Gao Qianqian , Wang Yu , Liu Hanmin , Chen Jiao 
Published: September 2021

Abstract
BackgroundAn ultrasonographic examination is widely used for prenatal screening of abnormal findings. The approach to the oral cavity of the fetus requires special attention because many aspects are unique and peculiar to this period of life. Obstetricians and pediatricians should be aware of the characteristics of normal patterns and be prepared to make a correct diagnosis. Congenital eruption cysts(EC) are rarely observed in fetuses and newborns, because at this stage of life, tooth eruption is unusual. Case presentationWe report a 26-year-old woman who had a sonographic examination at 27+5 weeks of pregnancy that showed a fetal oral cyst. During an ultrasound examination, we detected a hyperechogenic solid mass with a homogeneous texture. This mass was well circumscribed with a layer of anechoic area with no abnormal vessel. The cyst appeared to originate from the body of left mandible. Real-time assessment showed that the cyst was fixed and confined to inferior maxilla. Her neonate was finally diagnosed with EC. ConclusionUltrasound can reveal the distinctive features of oral cysts lesion and can be useful for the differential diagnosis. The treatment was required mainly by the symptomatology of the newborn. Therefore, prenatal ultrasonography could be used to conjecture the position and size of the mass, which is considered to be useful for localized and qualitative diagnosis prior to afterbirth treatment. Our findings indicate that ultrasound is an appropriate diagnostic tool to identify antenatal EC.
MicroRNA-29c-3p in Dual-Labeled Exosome is Potential Marker of Alzheimer's Disease
Authors: Li Ying , Xia Ming , Meng Shuang , Wu Di , Ling Sihai , Chen Xiali , Liu Chen-geng 
Published: September 2021

Abstract
ObjectiveWe aimed to establish a method to determine whether peripheral blood NCAM/amphiphysin 1 dual-labeled exosomal proteins and microRNA might be serve as a marker for the diagnosis of Alzheimer's disease.MethodsIt was a multicenter study using a two-stage design. The subjects included 45 SCD, 50 aMCI, 40 AD patients, and 30 controls in the discovery stage; the results were confirmed in the verification stage (47 SCD, 45 aMCI, 45 AD, and 30 controls). Peripheral blood NCAM single-labeled and NCAM/amphiphysin 1 double-labeled exosomes were captured and detected by immunoassay, respectively.ResultsLevels of Aβ42, Aβ42/40, Tau, P-T181-tau, and miR-29c-3p in plasma NCAM single-labeled and NCAM/amphiphysin 1 double-labeled exosomes of the aMCI and AD groups were significantly higher than those of the SCD group, control group and VaD group (all P<0.05). The levels of Aβ42 and miR-29c-3p in peripheral blood NCAM/amphiphysin 1 dual-labeled exosome was higher than that in the control and VaD groups (all P<0.05). The levels of exosomal Aβ42, Aβ42/40, Tau, P-T181-tau, and miR-29c-3p in peripheral blood were correlated with that in CSF (all P<0.05). Conclusion﻿This study first provided a method for sorting specific surface marker exosomes using a two-step immune capture technology. The plasma NCAM/amphiphysin 1 dual-labeled exosomal Aβ42/40 and miR-29c-3p had potential advantages in the diagnosis of SCD.
Association Analysis and Estimation of Breeding Values of Iranian Table Grapevine Germplasm by Integrating Pomological and Molecular Data
Authors: Razi Mitra , Darvishzadeh Reza , Baneh Hamed , Alipour Hadi 
Published: September 2021

Abstract
Genetic resources in each country are valuable assets for sustainable development. An accurate knowledge of genetic behavior and identification of genomic loci associated with important economic traits will help breeders to run efficiently their breeding programs. Fourteen important pomological traits were measured on 45 Iranian table grape cultivars during three successive years. Molecular profile of studied cultivars was prepared with 39 (Simple sequence Repeat) SSR primers pairs. Genetic structure analysis based on the SSR markers revealed two sub-populations (K = 2) in the association panel. In association analysis using mixed linear model seven loci were found to be significantly associated with the studied traits at 5% probability level. Breeding values were also estimated for the pomological traits using the best linear unbiased prediction (BLUP). “Saghal Solian”, “At Ouzum”, “Garmian”, “Rishbaba Qermez”, “Taifi”, “Shahroudi”, “Sahebi Qermez”, “Lal Qermez”, “Alhaghi”, “Sarghola”, “Chava Ga”, “Qzl Ouzum” and “Agh Shani” table grape cultivars showed high and positive breeding value for cluster length, width and weight. Whereas “Garmian”, “Rishbaba Qermez”, “Fakhri”, “Agh Shani”, “Lal Sefid” and “Shirazi” had positive and high breeding value for pollen germination, fruit set in open pollination and fruit set under controlled pollination. Finally, Sarghola” and “Qzl Ouzum” showed the positive and highest breeding value for berry weight, flesh weight, cluster length, cluster width and cluster weight. The cultivar with high and positive breeding value can be used as a good parent for breeding of traits in hybridization programs because they can better transfer the desirable characteristics to the progeny in each case.
Preconditioning with Melatonin Enhances Osteogenic Differentiation of Dental Pulp Mesenchymal Stem Cells by Regulating MAPK Pathways and Promotes the Efficiency of Bone Regeneration in Calvarial Bone Defects
Authors: Chan Ya-Hui , Ho Kuo-Ning , Lee Yu-Chieh , Chou Meng-Jung , Lew Wei-Zhen , Huang Haw-Ming , Lai Pin-Chuang , Feng Sheng-Wei 
Published: September 2021

Abstract
Background:Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival of engrafted stem cells remain challenging. Melatonin can regulate MSC biology. However, its function in the osteogenic differentiation of dental pulp-derived MSCs (DPSCs) remains unclear. We investigated the effects and mechanisms of melatonin preconditioning on the osteogenic differentiation and bone regeneration capacities of DPSCs.Methods:The biological effects and signaling mechanisms of melatonin with different concentrations on DPSCs were evaluated using a proliferation assay, the quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, a real-time polymerase chain reaction, and a western blot in vitro cell culture model. The in vivo bone regeneration capacities were assessed among empty control, MBCP, MBCP+DPSCs, and MBCP+DPSCs+melatonin preconditioning in four-created calvarial bone defects by using micro–computed tomographic, histological, histomorphometric, and immunofluorescence analyses after 4 and 8 weeks of healing.Results:In vitro experiments revealed that melatonin (1, 10, and 100 μM) significantly and concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity and extracellular calcium deposition, and osteogenic gene expression of DPSCs (p < 0.05). Melatonin activated the phosphorylation of RUNX-2 and OCN and inhibited COX-2/NF-κB phosphorylation. Furthermore, the phosphorylation of mitogen-activated protein kinase (MAPK) P38/ERK signaling was significantly increased in DPSCs treated with 100 μM melatonin, and their inhibitors significantly decreased osteogenic differentiation. In vivo experiments demonstrated that bone defects implanted with MBCP bone-grafting materials and melatonin-preconditioned melatonin exhibited significantly greater bone volume fraction, trabecular bone structural modeling, new bone formation, and osteogenesis-related protein expression than the other three groups at 4 and 8 weeks postoperatively (p < 0.05).Conclusions:These results suggest that melatonin promotes the proliferation and osteogenic differentiation of DPSCs by regulating COX-2/NF-κB and p38/ERK MAPK signaling pathways. Preconditioning DPSCs with melatonin before transplantation can efficiently enhance MSC function and regenerative capacities.
Risk score model of autophagy-related genes in osteosarcoma
Authors: Qin Wentao , Jiang Mingyang , Hu Yang , Xie Mingjing , Jike Yiji , Bo Zhandong 
Published: September 2021

Abstract
Background

Osteosarcoma (OS) is the most common primary malignancy in children and adolescents, with a high mortality and disability rate. Autophagy plays an important role in the regulation of apoptosis, invasion and metastasis of tumor cells. Hence, construction of a risk score model of autophagy related genes (ARGs) of OS would benefit the treatment and prognosis evaluation.
Methods

We downloaded a dataset of OS from The Cancer Genome Atlas (TCGA) database, and found the OS-related ARGs through Human Autophagy Database (HADb). Five hub ARGs (CCL2, AMBRA1, VEGFA, MYC and EGFR) were obtained by using multivariate Cox regression model. Then we calculated the risk scores and constructed a prediction model. Another two datasets downloaded from GEO were combined to verify the accuracy and validity of the model. The role of immune cell infiltration was systematically explored, and prediction of response to targeted drugs was assessed. Immunohistochemistry was carried out to verify the expression of the key ARGs.
Results

Based on these five hub ARGs, we constructed a risk score model related to OS. High accuracy and validity were demonstrated by datasets downloaded from GEO. These five ARGs played a role in cancer-related biological processes, such as MAPK pathway and PI3K pathway. The results of targeted drug sensitivity analyses coincided with the pathway analysis. Immunohistochemistry showed that the expression of 5 ARGs in OS group was more obvious than that in paracancerous group.
Conclusion

This study constructs a risk score model related to autophagy of OS, explores the prognostic value of autophagy related genes, and finds possible therapeutic targets.
The effect of the hypertensive phase on the long-term outcomes of Ahmed glaucoma valve (AGV) implantation
Authors: Nilforushan Naveed , Hassanpour Kiana , Vafaei Fatemeh , Yadgari Maryam 
Published: September 2021

Abstract
BackgroundTo investigate the long-term effect of hypertensive phase(HP) on the clinical outcomes of Ahmed Glaucoma Valve (AGV) implantation.MethodThe records of patients with different etiologies of glaucoma who underwent AGV implantation were retrospectively reviewed. HP was defined as the IOP> 21 mm Hg during the first three months after surgery. The main outcome measure was cumulative success defined as 5 < IOP< 21 mmHg and 20 % reduction from the baseline with or without IOP lowering medications. The secondary outcome measures were intraocular pressure (IOP) and the number of glaucoma medications. ResultsA total of 120 patients (28 patients of HP, 92 patients without HP) with an average age (±SD) of 48.9 ± 19.6 years and a mean follow-up of 4.5 ± 1.4 years were enrolled. The mean duration of survival was 5.3 ± 0.5 years in HP which was significantly shorter than 6.4 ± 0.2 years in non-HP. (log Rank= 4.2, P= 0.04) Mean IOP and number of IOP lowering agents were higher in postoperative visits at 1,2, 3, and 4 years in HP patients compared with non-HP. (All Ps < 0.01) Higher Baseline IOP was significantly associated with higher rates of surgical failure. Neovascular glaucoma was the only factor associated with the occurrence of HP in a logistic regression model. (Odds ratio: 3.14 CI: 1.2-8.1). Conclusion In the long-term follow up the duration of survival was significantly longer in the non-HP group. In the non-HP group, the success rate was significantly higher than the HP group
Novel Implementation of Multiple Mathematical Models in Automated Storage and Retrieval Warehouse Systems by Using Optimization Algorithms
Authors: Attari Mahdi Yousefi Nejad , Ala Ali , Jami Ensiyeh Neyshabouri 
Published: September 2021

Abstract
Warehouses and distribution centers are among the essential components in the supply chain, and their management is crucial. In the classifications made in the literature, warehouse management methods and distribution centers are divided into manual and machine forms. This system has advantages over previous systems, such as increased flexibility, accuracy, and order preparation speed. In this project, this storage system is called an automatic reverse storage system (ARWS). In this research, a complete and comprehensive explanation about the automatic reverse storage system and its components and their relationship with each other is provided. The following are mathematical models related to ordering items, assigning items to shelves, and completing orders and routing. For validation, the proposed models for small-size problems have been carefully solved with GAMS software. Then, to select the appropriate metaheuristic method, the landscape analysis method has been used. Hence using the analysis method, population-based methods were selected. Taguchi is used to determining the parameters of this method, and its results are presented.
Association of Immediate Reinsertion of New Catheters With Subsequent Mortality Among Patients With Suspected Catheter Infection: a Cohort Study
Authors: Zhong Yiyue , Deng Liehua , Zhou Limin , Liao Shaoling , Yue Liqun , Wen Shi Wu , Xie Rihua , Lu Yuezhen , Zhang Liangqing , Tang Jing , Wu Jiayuan 
Published: September 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
A Novel Waterborne Acrylic Coating Incorporation TIO2 Nanoparticles Modified With Silane Coupling Agent and Ag/zn Zeolite: Mechanical Properties, Thermal Stability, Artificial Weathering Durability and Antibacterial Activity
Authors: Dao Hung Phi , Nguyen Dung Tien , Nguyen Chinh Thuy , Nguyen Hiep Anh , Mac Phuc Van , Tran Trung Huu , Phung Lan Thi , Vu Trung Quoc , Vu Hieu Dinh , Ngo Quyen Thi Cam , Vu Cuong Manh , Nguyen Giang Vu , Tran Lam Dai , Thai Hoang 
Published: September 2021

Abstract
Effect of rutile TiO2 nanoparticles modified with 3-(trimethoxysilyl) propyl methacrylate silane - TMSPM (m-TiO2) and Ag/Zn zeolite on the properties, and durability of acrylic waterborne coatings was studied. The obtained results indicated that m-TiO2 nanoparticles and Ag/Zn zeolite could disperse regularly in the acrylic polymer matrix. Consequently, the abrasion resistance of the acrylic coating was improved in the presence of m-TiO2 or/and Ag/Zn zeolite. The abrasion resistance of the acrylic coating depended on the content of m-TiO2 nanoparticles as well as the initial TMSPM content for modification of rutile TiO2 nanoparticles. The abrasion resistance of the acrylic coating filled by m-TiO2 was higher than that of the acrylic filled by unmodified TiO2 nanoparticles. The acrylic coating filled by 2 wt.% TiO2 modified with 3 wt.% TMSPM had the highest abrasion resistance value, leveling off at 187.16 L/mil. The abrasion resistance of acrylic coating reduced with increasing too high initial TMSPM content for TiO2 modification and m-TiO2 nanoparticles content. The starting temperature of weight loss of acrylic coating filled by m-TiO2 was lower than that of the neat acrylic coating. In comparison with the m-TiO2 nanoparticles, the Ag/Zn zeolite particles showed a lower improvement for acrylic coating properties, i.e. abrasion resistance, weathering durability, but the Ag/Zn zeolite particles expressed an excellent antibacterial activity. Hence, combination of the m-TiO2 nanoparticles and Ag/Zn zeolite particles could enhance the properties of the acrylic waterborne coatings. The acrylic coating filled by 2 wt.% m-TiO2 nanoparticles and 1 wt.% Ag/Zn zeolite particles illustrated high abrasion resistance, good weather durability, and superior antibacterial activity for both of negative gram bacteria (E. Coli) and positive gram bacteria (S. Aureus). This coating is promising for environmental friendly building materials application.
Evaluation of Antimicrobial, Structural, Magnetic, and Thermal Potential of Silver Nanoparticles Anchored Polyaniline-Itaconic Acid-CuO Nanocomposites Synthesized From In-Situ Polymerization Method
Authors: E Parthiban , S Sudarsan 
Published: September 2021

Abstract
The silver nanoparticles (AgNPs) have been embedded within the itaconic acid (IA), polyaniline (PANi), and copper oxide (CuO) to form Ag@PANi-IA-CuO polymeric nanocomposites. In-situ polymerization of itaconic acid has been carried out in the presence of aniline monomers using anhydrous iron trichloride as an oxidising agent. The piper betel leaf extract was used to a reduction of AgNO3. The anchoring of AgNPs onto nanocomposite has been characterized using different techniques such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED) X-ray diffraction (XRD), and energy-dispersive X-ray (EDX). Biological, magnetic, and thermal properties of nanocomposites have also been studied in antimicrobial, vibrating sample magnetometer (VSM), and thermogravimetric analysis (TGA). Hence, these types of silver nanoparticles anchored polyaniline-itaconic acid-CuO nanocomposite has shown an attractive application in the field of biomedical and wastewater treatment.
Multi sensor data and temporal image fusion cross validation technique for Agri yield monitoring system
Authors: Shinde Swati V. , Shastri Rajveer , Dwivedi Atul Kumar , Haldorai Anandakumar , Sahni Varsha , Adusumalli Balaji 
Published: September 2021

Abstract
In recent years, the diverse application in various disciplines and the versatility has gained a huge interest for the researchers to research on the multi-sensor data fusion technology. The remote sensing process involves the measurement and recording of the data from a scene. Thus, the remote sensing systems are known to be a powerful tool as they help in the earth's atmosphere and surface monitor at different scales. The remote sensing of the data faces a serious challenge as the data captured by the multiple sensors are heterogeneous. This affects the efficient processing and the effectiveness of the data that is being sensed. Thus, the increase in the diversity in data increases the ancillary datasets. These multimodal datasets are used jointly to improve the processing performance as per the application requirement. Initially, the fusion of the temporal data with the backscattered/temporal data is possible from the data retrieved from remote sensing. Many researchers made several types of research on fusing the multi-temporal and multimodal data and gave different ideas for a different type of researchers. This paper presents the cross-validation technique for monitoring the yield. This monitoring system is developed by fusing the multi-sensor data and the temporal images. This fusion is performed, and the performance of the yield monitoring system is analyzed from the results obtained. By using the cross-validation technique, the efficiency of the system is found to be improved.
Triptolide exhibits anti-inflammatory effects on adipocytes and macrophages by inhibition of AMPK/mTOR pathway
Authors: Li Xue-li , Liu Zhao 
Published: September 2021

Abstract
Obesity is a growing global health problem and chronic over-nutrition disease with lipid accumulation that results in low-grade chronic inflammation in the microenvironment of adipose tissue. Triptolide is a diterpene lactone compound extracted from the roots of the Chinese herb TWHF and possesses a therapeutic potential due to its immunosuppressive and anti-inflammatory properties. In this study, we built obesity-related inflammatory models of adipocytes using LPS, Ma-CM and raw264.7 macrophages, while the obesity-related inflammatory models of macrophages were built using LPS and Ad-CM system. We used these inflammatory models to investigate the anti-inflammatory property of triptolide. Treatment of triptolide (0.005, 0.010, 0.020 and 0.040 μ M) inhibited LPS-induced or macrophages conditioned medium-stimulated activation of AMPK/mTOR signaling pathway (p < 0.05). The results showed that triptolide reduced the release of chemokines MCP-1, RANTES, EOTAXIN and KC in LPS, Ma-CM or RAW264.7 macrophages-stimulated 3T3-L1 adipocytes. Triptolide also diminished MCP-1, RANTES, EOTAXIN, KC and TNF-α in Ad-CM stimulated RAW264.7 macrophages, while expression of MCP-1, RANTES, TNF-α, GM-CSF and IL-6 was decreased in LPS stimulated RAW264.7 macrophages (p < 0.05). These results demonstrate that triptolide is not only effective against inflammatory response of RAW264.7 macrophages or 3T3-L1 adipocytes, but also disrupts the crosstalk between macrophages and adipocytes, particularly by inhibiting secretion of pro-inflammatory mediators through inhibiting the activation of AMPK/mTOR signaling pathway. Triptolide might benefit to ameliorate obesity-induced inflammatory diseases.