bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR perturbations at many coronary artery disease loci impair vascular endothelial cell functions
Authors: Wünnemann Florian , Tadjo Thierry Fotsing , Beaudoin Melissa , Lalonde Simon , Lo Ken Sin , Lettre Guillaume 
Published: February 2021

ABSTRACTGenome-wide association studies have identified 161 genetic variants associated with coronary artery disease (CAD), but the causal genes and biological pathways remain unknown at most loci. Here, we used CRISPR knockout, inhibition and activation to target 1998 variants at 83 CAD loci to assess their effect on six vascular endothelial cell phenotypes (E-selectin, ICAM1, VCAM1, nitric oxide, reactive oxygen species, calcium signalling). We identified 42 significant variants located within 26 CAD loci. Detailed characterization of the RNA helicaseDHX38and CRISPR activation at theFURIN/FES, CCDC92/ZNF664andCNNM2loci revealed a strong effect on vascular endothelial cell senescence.
Proxy-based model to assess the relative contribution of ballast water and biofouling’s potential propagule pressure and prioritize vessel inspections
Authors: Ceballos-Osuna Lina , Scianni Chris , Falkner Maurya , Nedelcheva Raya , Miller Whitman 
Published: February 2021

AbstractCommercial shipping is the primary pathway of introduction for aquatic nonindigenous species, mainly through the mechanisms of ballast water and biofouling. In response to this threat, regulatory programs have been established across the globe to regulate and monitor commercial merchant and passenger vessels to assess compliance with local requirements to reduce the likelihood of NIS introductions. Resource limitations often determine the inspection efforts applied by these regulatory agencies to reduce NIS introductions. We present a simple and adaptable model that prioritizes vessel arrivals for inspection using proxies for potential propagule pressure, namely a ships’ wetted surface area as a proxy for the likelihood of biofouling-mediated potential propagule pressure and ballast water discharge volume as a proxy for ballast water-mediated potential propagule pressure. We used a California-specific dataset of vessels that arrived at California ports between 2015 and 2018 to test the proposed model and demonstrate how a finite set of inspection resources can be applied to target vessels with the greatest potential propagule pressure. The proposed tool is adaptable by jurisdiction, scalable to different segments of the vessel population, adjustable based on the vector of interest, and versatile because it allows combined or separate analyses of the PPP components. The approach can be adopted in any jurisdiction across the globe, especially jurisdictions without access to, or authority to collect, risk profiling data or direct measurements for all incoming vessel arrivals.
Detection ofPlasmodiumsporozoites inAnopheles coustanis.l; a hindrance to malaria control strategies in highlands of western Kenya
Authors: Stephen Ayuya , Nicholas Kitungulu , Busula Annette O. , Webale Mark Kilongosi , Omukunda Elizabeth 
Published: February 2021

AbstractRe-emerging of high malaria incidences in highlands of western Kenya pose a challenge to malaria eradication efforts.Anopheles coustaniis a sub-Saharan mosquito species implicated in transmission of malaria in many parts of Africa as a secondary vector. It is a zoo-anthropophilic species that has been assumed to be of negligible importance. A cross sectional study was carried out in April to June, 2020 in Eluche location, Mumias East sub-County, Kakamega County, Kenya to establish the contribution ofAnopheles coustaniin malaria transmission. Pyrethrum spray collections (PSC) and Centers for Disease Control (CDC) and prevention light traps were used for sampling mosquitoes. Mosquitoes were collected from both indoors; between 0700h and 1100h using PSC and outdoors between 1800h and 0700h using CDC light traps. All mosquitoes were identified morphologically and femaleAnopheles’heads and thorax were analyzed further using Polymerase Chain Reaction (PCR) forPlasmodiumsporozoite. A total of 188 femaleAnophelesmosquitoes were collected from both PSC and CDC light traps. This constituted of; 80(42.55%)An. coustani, 52(27.66%)An. funestus, 47(25.00%)An. maculipulpis, 8(4.26%)An. arabiensisand 1(0.53%)An. gambiae. Malaria sporozoite detection was done to all theAnophelesfemale mosquitoes but only twoAn. coustanitested positive forPlasmodium falciparum. In conclusion,Anopheles coustaniplays a major role in outdoor malaria transmission in Mumias East Sub-County of Kakamega County in Western Kenya.
A rationally designed c-di-AMP FRET biosensor to monitor nucleotide dynamics
Authors: Pollock Alex J. , Choi Philip H. , Zaver Shivam A. , Tong Liang , Woodward Joshua J. 
Published: February 2021

ABSTRACT3’3’-cyclic di-adenosine monophosphate (c-di-AMP) is an important nucleotide second messenger found throughout the bacterial domain of life. C-di-AMP is essential in many bacteria and regulates a diverse array of effector proteins controlling pathogenesis, cell wall homeostasis, osmoregulation, and central metabolism. Despite the ubiquity and importance of c-di-AMP, methods to detect this signaling molecule are limited, particularly at single cell resolution. In this work, crystallization of theListeria monocytogenesc-di-AMP effector protein Lmo0553 enabled structure guided design of a Förster resonance energy transfer (FRET) based biosensor, which we have named CDA5. CDA5 is a fully genetically encodable, specific, and reversible biosensor which allows for the detection of c-di-AMP dynamics bothin vitroand within live single cells in a nondestructive manner. Our initial studies identify a unimodal distribution of c-di-AMP inBacillus subtiliswhich decreases rapidly when cells are grown in diluted Luria Broth. Furthermore, we find thatB. subtilismutants lacking either a c-di-AMP phosphodiesterase or cyclase have respectively higher and lower FRET responses, again in a unimodal manner. These findings provide novel insight into c-di-AMP distribution within bacterial populations and establish CDA5 as a powerful platform for characterizing new aspects of c-di-AMP regulation.ImportanceC-di-AMP is an important nucleotide second messenger for which detection methods are severely limited. In this work we engineer and implement a c-di-AMP specific FRET biosensor to remedy this dearth. We present this biosensor, CDA5, as a versatile tool to investigate previously intractable facets of c-di-AMP biology.
Comprehensive imaging of synaptic activity reveals dendritic growth rules that cluster inputs
Authors: Podgorski Kaspar , Toth Tristan Dellazizzo , Coleman Patrick , Opushnyev Serhiy , Brusco Janaina , Hogg Peter , Edgcumbe Philip , Haas Kurt 
Published: February 2021

AbstractThe distribution of synapses across dendritic arbors determines their contribution to neural computations since nonlinear conductances amplify co-active clustered inputs. To determine whether, and how patterned synaptic topography arises during development we developed a random-access microscope capable of full-neuron calcium imaging of activity and structural plasticity of developing neurons in awakeXenopustadpoles. By imaging growing brain neurons in response to plasticity-inducing visual training, we show coordinated growth and synaptogenesis specific to each neuron’s spike tuning. High evoked activity in neurons tuned to the trained stimulus induced pruning of non-driven inputs across the dendritic arbor as these neurons strengthened their responses to this stimulus. In stark contrast, initially unresponsive neurons that shifted their spike tuning toward the trained stimulus exhibited localized growth and new responsive synapses near existing active inputs. These information-driven growth rules promote clustering of synapses tuned to a developing neuron’s emerging receptive field.One-Sentence SummarySensory input directs brain neuronal growth and connectivity promoting clustering of synaptic inputs tuned to a neuron’s encoding properties.
Numerical and experimental analysis of performance in a compact plate heat exchanger using graphene oxide/water nanofluid
Authors: Singh Shiva , Verma Piyush , Ghosh Subrata Kumar 
Published: February 2021

Purpose
This study aims to present the experimental and computational performance analysis in compact plate heat exchanger (PHE) using graphene oxide nanofluids at different concentrations and flow rate.

Design/methodology/approach
Field emission scanning electron microscope and X-ray diffraction were used to characterize graphene oxide nanoparticles. The nanofluid samples were prepared by varying volume concentration. Zeta potential test was done to check stability of samples. The thermophysical properties of samples have been experimentally measured. The experimental setup of PHE with 60° chevron angle has also been developed. The numerical analysis is done using computational fluid dynamics (CFD) model having similar geometry as of the actual plate. Distilled water at fixed temperature and flow rate is used in hot side tank. Nanofluid at fixed temperature with varying concentration and flow rate is used in cold side tank as coolant.

Findings
The numerical and experimental results were compared and found that both results were in good agreement. The results showed ∼13% improvement in thermal conductivity, ∼14% heat transfer rate (HTR), ∼9% in effectiveness and ∼10% in overall heat transfer coefficient at cost of pressure drop and pumping power using nanofluid. Exergy loss also decreased using nanofluid at optimum concentration of 1 Vol.%.

Originality/value
The CFD model can be significant to analyze temperature, pressure and flow distribution in heat exchanger which is impossible otherwise. This study gives ease to predict PHE performance with high accuracy without performing the experiment.
Contrasting supply chain traceability and supply chain visibility: are they interchangeable?
Authors: Roy Vivek 
Published: February 2021

PurposeSupply chain traceability and supply chain visibility have become a critical element for the effective management of contemporary complex supply chains. At their core is information sharing, which has been acknowledged as a key prerequisite for logistics and supply chain performance, but whose notional underpinnings have not been delineated fully, leading to interchangeable deployment of these terms. Addressing the shortcoming, this paper aims to establish a contrast between the two notions.Design/methodology/approachDrawing from systematic review protocols, a multi-disciplinary review scope is constructed wherein the synthesis is strategized to primarily channel implications for the scholarship of logistics and supply chain management. The review is aimed at addressing two research objectives: (1) how the notions of traceability and visibility in supply chain management develop contrast in terms of their thematic emphasis and (2) to attain an integrative understanding of the notional convergence and divergence between supply chain traceability and visibility for raising strategic recommendations.FindingsThe review outcomes help contrast both the convergence and the divergence between traceability and visibility in the supply chain environment, and the differentiated but fundamental role that information sharing plays within these notions to outline why they are not interchangeable.Originality/valueThe originality of the findings lies in the conceptual synthesis of the relevant literature from both technological and non-technological perspectives to ultimately draw logistics and supply chain management implications. The review also points out key strategic considerations to demarcate the notional boundaries of traceability and visibility in future research.
Outward FDI and productivity spillovers in China: an industrial perspective
Authors: Ali Usman , Li Yanxi , Wang Jian-Jun , Chen Zhen 
Published: February 2021

PurposePrior research demonstrated that China's Outward FDI (OFDI) is aimed at sustaining long-term economic growth by promoting industrialization and technological upgrading in the country. However, empirical evidence on the effectiveness of this strategy remains scarce. This study intends to fill this gap by exploiting endogenous changes in industrial productivity stemming from OFDI to examine if China's new strategy to spur OFDI is economically beneficial for the industries involved.Design/methodology/approachThe authors employed the two-step system-GMM and pooled mean group approaches on a panel dataset of 18 Chinese industries over the 2004–2017 period. The industrial sectors are further classified into the state dominated and non-state dominated ones to evaluate whether the productivity growth impact of OFDI varies by the level of ownership structure. Besides, the dataset is further decomposed into the ex ante and ex-post BRI era to test if this initiative has altered the underlying relationship.FindingsThe results provide robust evidence that China's OFDI through reverse spillover effects promotes productivity growth in the domestic industries, and such productivity gains are greater for the non-state dominated industries, and the OFDI in the BRI era. The findings suggest that OFDI can act as a catch-up strategy to release excess capacity and acquire technology and smart business practices.Originality/valueThis study is the first attempt to highlight the reverse productivity spillovers associated with OFDI at the industrial level. The study's findings guide the government officials and the practitioners of foreign investment to better understand the implications of their investment projects in terms of technology improvements and to optimize market opportunities.
Undue pressure or moral obligation: religious gifting and undue influence
Authors: Kennedy Vinny 
Published: February 2021

PurposeThis paper aims to stimulate discussion on how best to protect individuals from undue influence when gifting to religious institutions in England and Wales.Design/methodology/approachThis paper is based on the law relating to undue influence in England and Wales and draws from the literature regarding gifting to religious institutions and accountability of such institutions.FindingsThis paper identifies that more needs to be done to protect individuals, so as to ensure they are gifting to religious institutions using their own free will. It highlights that although the law attempts to define undue influence, there is little guidance on where the line between persuasion and coercion lies. The paper recognises that religious institutions need to do more to adopt safeguarding policies and that the Charity Commission ought to better support such policies by creating a single point of reference.Practical implicationsSteps need to be taken to prepare a cohesive set of principles that religious institutions of all denominations can follow to ensure they protect themselves from being accused of undue influence, as well as safeguarding individuals from abuse.Originality/valueThere are limited studies that consider the dichotomy between religious gifting and undue influence. This paper adds to the existing discussion and considers ways in which individuals can be protected. The author is not aware of such considerations being directly contemplated as resolutions to this issue.
Emotional labor in a sales ecosystem: a salesperson-customer interactional framework
Authors: Klein Michel 
Published: February 2021

PurposeThe concept of emotional labor refers to the management of emotions in interaction with customers. This study aims to suggest an integrative definition of emotional labor. It develops a conceptual framework that helps organize and synthesize key insights from the literature, in an interactional and multi-level perspective.Design/methodology/approachThis integrated framework consists in a mapping of key research themes resulting from a systematic literature review, which includes research in sales and marketing. As critical affective processes in sales have not been studied sufficiently, both in business-to-business and business-to-customer selling, this review also incorporates works in other research fields.FindingsSales representatives’ emotional labor must be considered as a bi-directional interaction with the customer in a multi-level perspective. Moreover, emotional labor has rather negative consequences for the salesperson (e.g. burnout and job stress), but may have positive sales and customer outcomes. Findings suggest that the expression of genuine emotions should be used during sales interactions. In addition, organizations should prevent customers’ negative behaviors (e.g. mistreatment).Practical implicationsEmotional labor key practical implications with regard to several management functions such as the recruitment, performance management and training (Ashkanasy and Daus, 2002) of the sales representatives.Originality/valueResearch on emotional labor in a sales ecosystem is scarce. It has largely covered service industry employees in contact with customers, but has not paid enough attention to sales representatives (Mikeskaet al., 2015). The proposed integrated framework concerning emotional labor focuses on the bi-directional interaction between the sales representatives and their customers.
Determinants of corporate social and environmental voluntary disclosure in Saudi listed firms
Authors: Boshnak Helmi A. 
Published: February 2021

PurposeThis paper aims to examine firm characteristics and ownership structure determinants of corporate social and environmental voluntary disclosure (CSEVD) practices in Saudi Arabia to address the paucity of research in this field for Saudi listed firms.Design/methodology/approachThe paper uses manual content and regression analyses for online annual report data for Saudi non-financial listed firms over the period 2016–2018 using CSEVD items drawing on global reporting initiative-G4 guidelines.FindingsModels show that Saudi firm CSEVD has increased over time compared to previous studies to an average of 68% disclosure due to new corporate governance regulations and IFRS implementation. The models show that firm size, leverage, manufacturing industry type and government ownership are positive determinants of CSEVD, while family ownership is the negative driver of CSEVD. However, firm profitability, audit firm size, firm age and institutional ownership have no impact on the level of CSEVD.Originality/valueUsing legitimacy and stakeholder theories, the paper determines the influence of firm characteristics and ownership structure on CSEVD, identifying implications for firm stakeholders and providing some evidence on the impact of corporate governance regulation and IFRS implementation on such disclosure. The paper provides additional evidence on progress towards Saudi’s Vision 2030.
Institutional excellence reloaded: a 17-years, two-phase in-depth study of corporate culture change in the health care sector
Authors: Beil-Hildebrand Margitta B. 
Published: February 2021

PurposeThis ethnographic revisit of a general hospital aims to critically explore and describe the mechanisms of corporate culture change and how institutional excellence is facilitated and constrained by everyday management practices between 1996/1997 and 2014/2015.Design/methodology/approachA five-month field study of day-to-day life in the hospital's nursing division was conducted by means of an ethnographic revisit, using participant-observation, semi-structured interviews, free conversations and documentary material.FindingsUsing labour process analysis with ethnographic data from a general hospital, the corporate culture is represented as faceted, complex and sophisticated, lending little support to the managerial claims that if corporate objectives are realised, they are achieved through some combination of shared values, beliefs and managerial practices. The findings tend to support the critical view in labour process writing that modern managerial initiatives lead to tightened corporate control, advanced employee subjection and extensive effort intensification. The findings demonstrate the way in which the nursing employees enthusiastically embrace many aspects of the managerial message and yet, at the same time, still remain suspicious and distance themselves from it through misbehaviour and adaptation, and, in some cases, use the rhetoric against management for their own ends.Practical implicationsWhat are the implications for clinical and managerial practitioners? The recommendations are to (1) develop managerial practitioners who are capable of managing change combined with the professional autonomy of clinical practitioners, (2) take care to practise what you preach in clinical and managerial reality, as commitment, consent, compliance and difference of opinion are signs of a healthy corporate culture and (3) consider the implications between social structures and human actions with different work behaviours on different levels involved.Originality/valueThis ethnographic revisit considers data from a labour process analysis of corporate culture change in a general hospital and revisits the ways in which contradictory expectations and pressures are experienced by nursing employees and management practitioners spread 17 years apart.
Role of halal literacy and religiosity in buying intention of halal branded food products in India
Authors: Khan Adil , Arafat Mohd Yasir , Azam Mohammad Khalid 
Published: February 2021

Purpose
This study aims to investigate the influence of religiosity (intrinsic and extrinsic) and halal literacy on the intention of Muslim consumers to purchase halal branded food products in India. An extended version of the theory of planned behaviour (TPB) was used as a framework. Apart from religiosity and halal literacy, the influence of attitude, social norms and perceived behavioural control of halal on buying intention were also tested.

Design/methodology/approach
The study uses a survey design. The data were collected from 350 individual respondents, using a closed-ended, structured questionnaire. The quality of the measurement model has been assessed through reliability testing, factor loading, average variance extracted and Fornell-Larcker criterion. The test of hypotheses was conducted by performing the partial least square structural equation modelling.

Findings
The result of hypotheses testing shows that both intrinsic and extrinsic types of religiosities did not have a direct influence on buying intention. However, religiosity (extrinsic and intrinsic) and halal literacy have significant relationships with most of the antecedents of the intention of the TPB. In addition, both kinds of religiosities (extrinsic and intrinsic) and halal literacy had a significant indirect effect (through TPB antecedents) on buying intention.

Originality/value
Muslim population in India is one of the largest in the world, yet there is a lack of popular halal branded food products in the market. Nevertheless, few researchers have attempted to study the consumer behaviour of the Muslim population for halal products in India. A large amount of research work on halal food behaviour is from countries such as Malaysia and Indonesia, where the Muslim population is in the majority and halal brands are already popular. Further, this paper studies the impact of dimensions of religiosity, which has been overlooked by researchers studying the halal food purchasing behaviour. The study also explores the impact of halal literacy, an understudied construct in halal marketing literature. The present study is amongst the earliest empirical research based on Muslim consumers in India on the topic of halal branded food products.