bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moderate Intensity Treadmill Running Relieves Motion-Induced Post-Traumatic Osteoarthritis Mice by up-Regulating the Expression of lncRNA H19
Authors: Zhou Xuchang , Cao Hong , Wang Miao , Zou Jun , wu wei 
Published: July 2021

Abstract
Background The purpose of this study was to explore whether moderate-intensity exercise can alleviate motion-induced post-traumatic osteoarthritis (PTOA) and the expression change of lncRNA H19 during this progression.Methods Twenty-week-old male C57BL/6 mice were randomly divided into five groups: model control group (CM group, n = 6), treadmill model group (M group, n = 6), rehabilitation control group (CK group, n = 6), treadmill model + rehabilitation training group (K group, n = 6) and treadmill model + rest group (J group, n = 6). Paraffin sections were used to observe the pathological changes in the mouse knee joint in each group. A micro-CT was used to scan the knee joint to obtain the morphological indexes of tibial plateau bone. Real-time PCR was used to detect the mRNA levels of inflammatory factors, synthetic and catabolic factors in cartilage. Results After high-intensity exercise for four weeks, the inflammation and catabolism of the mouse knee cartilage were enhanced, and the anabolism was weakened. Further study showed that these results were partially reversed after four-week moderate-intensity training. The results of hematoxylin-eosin staining confirmed this finding. Meanwhile, high-intensity exercise reduced the expression of lncRNA H19 in cartilage, while the expression of lncRNA H19 increased after four weeks of moderate-intensity exercise.Conclusion High-intensity treadmill running can cause injury to the knee cartilage in C57BL/6 mice which leads to PTOA and an decrease of lncRNA H19 expression in cartilage. Moderate-intensity exercise can relieve PTOA and partially reverse lncRNA H19 expression.
Sorption And Physicochemical Properties of A Phosphorylated Mercerised Cotton Cellulose
Authors: Solovov Roman , Perevoznikova Anfisa Yu. , Seliverstvov Alexander F. , Shapagin Alexey V. , Fedoseev Alexander M. , Milutin Vitaly V. , Ershov Boris G. 
Published: July 2021

Abstract
A process of a mercerised cotton cellulose sample phosphorylation has been investigated. After oxidation a phosphorus content was determined by spectrophotometric analysis and it was in a range of 0.179 to 0.950 mmol g–1. A significant decrease in the tensile strength of the sample was found upon an increase of phosphoric acid concentration in a phosphorylating solution. Phosphorylated mercerized fabric contents more phosphorous as unmercerized fabric in 3 times. The sorption properties of phosphorylated cotton cellulose in aqueous solution containing Cu2+ were characterized. The maximum of static exchange capacity was found to be 1.48 ± 0.11 mmol g–1 for phosphorylated cotton with content 0.898 ± 0.090 mmol g–1 of phosphorus. The sorption of Cu2+ by single phosphorus-containing group occurs for samples with not exceeding 0.80 mmol g–1 of phosphorus. The preliminary studies of uranium(VI) micro quantities of radionuclides 241Am, 233U and 239Pu sorption from aqueous solution with phosphorylated textile demonstrated the high efficiency.
Genetic Relationship Between Interleukın-6 rs1800796 Variants, Interleukın-6 Plasma Levels and Susceptibility to Type 2 Diabetes Mellitus and Diabetic Nephropathy
Authors: COBAN Nezaket , Bayramoglu Aysegul , TEMIZ Zeynep 
Published: July 2021

Abstract
Type 2 diabetes mellitus (T2DM) is very common worldwide and genetically heterogeneous. One of the microvascular complications is diabetic nephropathy (DN). In recent years, T2DM has been described as a disease caused by chronic inflammation. The imbalance between pro- and anti-inflammatory cytokines causes inflammation. One of the candidate genes associated with T2DM and DN is the Interleukin-6 (IL-6) gene, one of the pro-inflammatory cytokines. This study was conducted to determine the polymorphism frequencies of the IL-6 gene rs1800796 and investigate the role of this polymorphism in the development of T2DM and DN. Genomic DNA that was obtained from 261 people was used in the study. IL-6 gene rs1800796 polymorphism was determined using the PCR, restriction fragment length polymorphism (RFLP) and electrophoresis. IL-6 gene PCR products were discontinued by treatment with restriction enzyme BsrBI and were analyzed in 2% agarose gel electrophoresis. IL-6 (Bioassay technology laboratory, Shangai, China) level was examined by enzyme-linked immunosorbent assay (ELISA) using a commercial kit. The results were statistically analyzed. The frequencies of rs1800796 genotypes were found to be GG 70.7%, GC 28.5%, CC 0.8% in the control group and GG 87.8%, GC 9.9 %, CC 2.3% in T2DM patients. Although there was a statistically significant difference between the control group and the T2DM patient group in genotype and allele frequencies, there was no significant difference in DN. The G allele frequency was also significantly higher in the T2DM group (p=0.000). IL-6 levels were determinated increased in patients with Type-2 diabetes compared to the control group. However; there was no significant statistically. We can say that IL-6 rs1800796 polymorphism is related to T2DM and G allele can be used as a useful genetic marker; this polymorphism is not related to DN, though.
Analyzing Sex-Differences in Atrial Fibrillation Patients: Bias or Proper Management?
Authors: Israeli Asaf , Gal Danna , Younis Autba , Ehrenberg Scott , Rozner Ehud , Turgeman Yoav , Koren Ofir 
Published: July 2021

Abstract
Background: There are inconsistent and conflicting data among males and females with AF. Objective: This study intends to analyze whether the sex-based differences among AF patients were influenced by age, co-morbidities, and treatment strategy rather than solely gender difference. Methods: We analyzed 327 consecutive patients admitted to the ED due to AF for three years and follow-up for a year. Results: Females with AF were older (p<.001), had higher BMI (p<.001), and a higher rate of co-morbidities as hypertension (p<.001), hyperlipidemia (p=0.01), Diabetes mellitus (p=0.05), valvular heart disease (p=0.05) and thyroid dysfunction (18.3% vs. 1.8%, p<.001). AF males had a higher rate of coronary disease (p<.001) and heart disease with reduced ejection fraction (p<.001). As a result, the mean CHADS2 and CHA2DS2-VASc scores were significantly higher in females (p<.001 for both). Female tends to be treated with rate control medications and less with antiarrhythmic agents (p<.001). Univariate analysis reveals that females had a higher rate of recurrent AF, heart failure hospitalization, CVA, and myocardial infarction. Yet, adjusting gender to age and co-morbidities shows that the females remain to have a higher rate of heart failure hospitalization (OR 2.73 95%CI 1.04-5.89, P-value <.001) and recurrent AF (OR 3.86, P-value =0.02). Thyroid dysfunction and the lack of antiarrhythmic treatment significantly increase the risk of AF (OR 5.95 95%CI 3.15-9.73, OR 3.42, respectively, P-value<.001 for both) regardless of gender. The mortality rate differs only in a sub-group of females ≥ 75 years of age (OR 1.60, P<.001). Conclusion AF males and females differ significantly in baseline characteristics. Females are older, have more co-morbidities, and tend to be treated unnecessarily differently for AF. Following age and co-morbidities adjustments, a female gender remains significant for heart failure hospitalization and recurrent AF. Thyroid dysfunction and AF treatment may explain the sex-based difference of recurrent AF.
The Involvement of PPARγ In Anti-Inflammatory Activity of N-Stearoylethanolamide
Authors: Kosiakova Halyna , Berdyshev Andrii , Dosenko Victor , Drevytska Tetyana , Herasymenko Oleksandra , Hula Nadiya 
Published: July 2021

Abstract
N-stearoylethanolamide (NSE)– a cannabinoid-like compound with wide range of biological activity. Anti-inflammatory properties of NSE have been indicated on different animal models of pathological conditions. However, the molecular mechanisms of anti-inflammatory action of NSE remain unclear. In the current study, the involvement of PPARγ in the NF-kB -dependent anti-inflammatory action of NSE was evaluated using different methodological approach. First method - molecular modeling, evaluated the possibility of NSE to bind with PPAR. Then, in ex vivo experiment, using selective synthetic agonist of PPARα/γ LY-171883 and selective antagonist of PPARγ - GW9662, the role of PPARα /PPARγ in the NSE’s effect on nuclear NF-kB translocation was examined in LPS-activated rat peritoneal macrophages. Finally, the NSE action on the mRNA level of several PPARγ- dependent genes was studied in liver of insulin-resistant rats. The molecular docking results showed that NSE could bind to PPARγ and compete for the binding with antagonist GW9662 and agonist LY171883 in the active site of PPARγ. It also has been found that NSE prevented the LPS-induced NF-kB translocation into the nuclei of rat peritoneal macrophages during pre-treatment with NSE before LPS application. When NSE was added before GW9662 and LPS treatment, the level of NF-kB translocation and IL-1β content reduced to control cells’ levels. These data confirmed a competitive binding of NSE with GW9662 for the ligand-binding domen of PPARγ. In addition, NSE administration to insulin resistant rats changed the mRNA expression of several PPARγ target gens, including FATP1 and IL1-ra.
Repair of Damaged Parts Using Wire Arc Additive Manufacturing In Machine Tools Remanufacturing
Authors: Lee Jeong-Hak , Lee Choon Man , Kim Dong-Hyeon 
Published: July 2021

Abstract
Mechanical parts manufactured using additive manufacturing (AM) processes are used in various fields, such as the aerospace, automobile, and machine tool fields. Recently, many researchers have been actively researching AM processes, owing to their advantages in fabricating complex-shaped components that are difficult to process using conventional processes such as rolling, forging, and casting. One of the AM processes, wire arc additive manufacturing (WAAM), is widely used for manufacturing and repair. Mechanical parts usually suffer from severe damage, such as wear and cracks, during operation. Therefore, research on the damaged parts is often required for repairs. This study was performed to investigate the repair of damaged parts using WAAM in machine tool remanufacturing. In general, these parts are often damaged owing to falls of the workpieces and the inexperience of workers; the cross slide is one of the parts damaged most often in a lathe. Accordingly, this study used the cross slide as an example. First, the damaged area was partially cut flat. Second, it was filled with a metal wire using WAAM. Third, post-processing was performed to adjust the surface quality and shape accuracy. Then, the microstructure, chemical composition, and mechanical property of the repaired part were analyzed. Finally, the repaired cross slide was used to replace damaged parts. In general, this method can be applied to various mechanical parts such as impellers, turbine blades, and machine tools.
Novel Core-Shell Non-Sintered Lightweight Aggregate Concrete for Better Properties in Wallboard
Authors: PANG Chaoming , MENG Xinxin , ZHANG Chunpeng , PAN Jinlong 
Published: July 2021

Abstract
Shrinkage of foam concrete can easily cause cracking and thus makes it difficult for a manufacturer to maintain quality. The density of lightweight aggregate concrete is too high to meet specifications for lightweight and thermal insulation for wallboard. Two types of concrete with dry density in the range 1000–1200 kg/m3 for use in wallboard were designed and prepared using foam and lightweight aggregate. The properties of porous lightweight aggregate concrete with core-shell non-sintered lightweight aggregate were compared with sintered lightweight aggregate concrete along with several dimensions. The two aggregates were similar in particle size, density, and strength. The effects of each aggregate on the workability, compressive strength, dry shrinkage, and thermal conductivity of the lightweight concrete were analyzed and compared. Pore structures were determined by mercury intrusion porosimetry and X-ray computed tomography. Compressive strength ranged from 7.8 to 11.8 MPa, and thermal conductivity coefficients ranged from 0.193 to 0.219 W/m/K for both types of concrete. The results showed that the core-shell non-sintered lightweight aggregate bonded better with the paste matrix at the interface transition zone and had a better pore structure than the sintered lightweight aggregate concrete. Slump flow of the core-shell non-sintered lightweight aggregate concrete was about 20% greater than that of the sintered lightweight aggregate concrete, 28d compressive strength was about 10% greater, drying shrinkage was about 10% less, and thermal conductivity was less. Porous lightweight aggregate concrete using core-shell non-sintered lightweight aggregate performs well when used in wallboard because of its low density, high thermal insulation, and improved strength.
Myeloperoxidase as an Important Predictor of Cardiovascular Risk in Individuals with Rheumatoid Arthritis
Authors: Montes Elisangela Gueiber , Mansani Fabiana Postiglioni , Júnior Alceu de Oliveira Toledo , Schafranski Marcelo Derbli , Zardo Bruno Queiroz , Santos Fábio André dos , Grassiolli Sabrina , Calixto Lorena de Freitas , Costa Romulo Lopes da , Netto Felício de Freitas , Vellosa José Carlos Rebuglio 
Published: July 2021

Abstract
Background: Rheumatoid arthritis is an inflammatory disease with joint manifestations. In the presence of extra-articular manifestations, the morbidity and severity of the disease increases. Glucocorticoid is used as a treatment and may result in side effects related to cardiovascular risk. Methods: This was a cross-sectional study including 59 volunteers with rheumatoid arthritis receiving treatment at a Hospital of Campos Gerais, that aimed to establish the relation between cardiovascular risk, glucocorticoid treatment and myeloperoxidase in these patients. Subjects were divided into two groups: using (n = 39) and without glucocorticoids (n = 20). They underwent clinical evaluation, physical examination and blood samples were taken. Statistical analysis was performed using Student's t-test and Mann-Whitney test. Logistic regression was performed to assess cardiovascular risk. The significance level was 5% (α = 0.05). Calculations were performed using the Statistical Package for the Social Science version 21.0. Results: There has been a significant difference between groups in blood glucose values (p = 0.012), that can be explained by the different percentage of diabetic patients in the groups. When assessed cardiovascular risk using the predictors of glucocorticoid dose, time of glucocorticoid use, myeloperoxidase, and C-reactive protein together, these were responsible for significantly predicting cardiovascular risk (p = 0.015). Conclusions: A significant relation between the predictor myeloperoxidase alone was also demonstrated (p = 0.037), may it be an important predictor of cardiovascular risk among individuals with rheumatoid arthritis.
Airway Management for Penetrating Head and Neck Trauma, Experience of a Trauma Center from 2012 to 2020
Authors: Morvan Jean-Baptiste , Cungi Pierre-Julien , Cotte Jean , cassignol arnaud , Renard Aurélien , Bussone Claire-Marie , Maso Victorine , Pascaud Damien , Beucler Nathan , Vatin Loraine , Rivière Damien , Joubert Christophe , Cathelinaud Olivier , Bordes Julien 
Published: July 2021

Abstract
BackgroundFrom a series of penetrating head and neck trauma managed in a level-1 Trauma Center, the main aim of this study was to determine predictive factors for early definitive airway management, during pre-hospital time or in the emergency room. The secondary objective was to perform a descriptive epidemiological analysis of the series.MethodsA single-center retrospective study was conducted between January 1 2012 and June 30 2020. in a French Level 1 Trauma Center. The patients included were adults treated for penetrating head and neck trauma, regardless of the mechanism and the causal agent.Results 56 patients were included. Ballistic origin, Shock Index >0.9 and active bleeding in the emergency room were predictive criteria for definitive airway management during pre-hospital time or in the emergency room. 78.6% of patients were male. Median age was 54 years. The trauma followed a suicide attempt in 50% of cases, an accident in 26.7% and an assault in 23.2%, with use of a knife in 42.9% and firearm in 26.8%. Mortality was 10.7%. 16.1% of patients had undergone pre-hospital intubation and 19.1% intubation in the emergency room. CT scan was performed in 87.5% of cases, surgery in 96.4% and tracheotomy in 37.5%. A laryngotracheal lesion was seen in 14.2%. In 50% of patients, primary admission was to intensive care. Conclusions Ballistic origin, Shock Index >0.9 and active bleeding in the emergency room were predictive criteria for early definitive airway management. This study established the profile of patients suffering from penetrating head and neck trauma managed in a Trauma Center over a period of 9 years.
Integrated RNAi Screening Identifies The NEDDylation Pathway As A Synergistic Partner of Azacytidine In Acute Myeloid Leukemia
Authors: Klosner Justine , Agelopoulos Konstantin , Rohde Christian , Göllner Stefanie , Schliemann Christoph , Berdel Wolfgang E. , Müller-Tidow Carsten 
Published: July 2021

Abstract
Treatment of Acute Myeloid Leukemia (AML) remains challenging and novel targets and synergistic therapies still need to be discovered. We performed a high-throughput RNAi screen in three different AML cell lines and primary human leukemic blasts to identify genes that synergize with common antileukemic therapies. We used a pooled shRNA library that covered 5043 different genes and combined transfection with exposure to either azacytidine or cytarabine analog to the concept of synthetic lethality. Suppression of the chemokine CXCL12 ranked highly among the candidates of the cytarabine group. azacytidine in combination with suppression of genes within the neddylation pathway led to synergistic results. NEDD8 and RBX1 inhibition by the small molecule inhibitor pevonedistat inhibited leukemia cell growth. These findings establish an in vitro synergism between NEDD8 inhibition and azacytidine in AML. Azacytidine and NEDD8 inhibitors are currently undergoing clinical trials in combination with azacytidine. Taken together, neddylation constitutes a suitable target pathway for azacytidine combination strategies.
Increased Survival In Puppies Affected By Canine Parvovirus Type II Using An Immunomodulator As A Therapeutic Aid
Authors: Muñoz Adriana I , Vallejo-Castillo Luis , Fragozo Ana L , Vazquéz-Leyva Said , Pavón Lenin , Pérez-Sánchez Gilberto , Soria-Castro Rodolfo , Mellado-Sánchez Gabriela , Cobos-Marin Laura , Pérez-Tapia Sonia Mayra 
Published: July 2021

Abstract
Background: The canine parvoviral enteritis (CPE) promotes sepsis and systemic inflammatory response syndrome (SIRS). Mortality in this disease is usually registered within 48–72 h post-hospitalization, the critical period of the disease. It has been recently described that the use of an immunomodulator whose major component is monomeric ubiquitin (mUb) without the last two glycine residues (Ub∆GG) in pediatric patients with sepsis improves survival. It is known that CXCR4 is the cell receptor of extracellular ubiquitin in humans. The aim of this work was to explore the effect of one immunomodulator (hDLE) as a therapeutic auxiliary in CPE puppies with sepsis and SIRS.Results: We studied two groups of puppies with CPE confirmed by polymerase chain reaction. The first group received conventional treatment (CT) and vehicle (V), while the second group received CT plus an immunomodulator (I). In both groups, we assessed the survival, clinical condition, number of erythrocytes, neutrophils, and lymphocytes, and values of hematocrit, hemoglobin, plasma proteins, cortisol, and norepinephrine epinephrine, and serotonin, during hospitalization.Puppies treated with CT+I showed 81% survival and milder clinical signs and a significant decrease in circulating neutrophils and lymphocytes in the critical period of the treatment. In contrast, the CT+V group presented a survival of 42%, severe clinical status, and no improvement of the parameters evaluated in the critical period of the disease. We determined in silico that human Ub∆GG can stimulate dog CXCR4.Conclusions: The administration of an immunomodulator (5 mg/day x 5 days) to puppies with CPE under 6 months of age reduces the severity of clinical signs, increases survival, and modulates inflammatory cell parameters. Further studies are necessary to take full advantage of these clinical findings which might be mediated by the human Ub∆GG to canine CXCR4 interaction.
Impacts of Short-Term Water Restriction on Pelibuey Sheep: Physiological and Blood Parameters
Authors: Serrano Jorge Orlay , Villares-Garachana Asiel , Correa-Herrera Nelson , González-Morales Abel , Pérez-Bonachea Lisbet , Hernández Lázaro , Lorente Gustavo , Hajari Elliosha , Fonseca-Fuentes Norge , Martínez-Melo Jorge , Lorenzo José Carlos 
Published: July 2021

Abstract
One of the projected effects of climate change is a reduction in rainfall in certain regions of the world. Hence, the agricultural and livestock sectors will have to cope with increasing incidences of water shortage whilst still maintaining productivity levels to feed an ever increasing global population. This short communication reports on the effect of a two week water stress on Pelibuey sheep in Cuba. Three treatments were compared viz. supply of water ad libitum; water supplied once every 3 or 6 d. Following exposure to the water stress, the results showed no changes in sheep body weight or rectal temperature. However, respiration frequency was affected with water stress causing a reduction from 23.3 to 13.3 respirations per min in control and water deprived animals, respectively. Furthermore, there was evidence for hemoconcentration in response to water stress (levels of hemoglobin increased from 9.2 to 13.1 g L-1 and hematocrits from 27.6 to 39.3% in the control group and animals restricted to water once every 6 d. The imposed water stress was also evident in the reduction of lymphocytes (from ±63 to 43%), and in increase of neutrophils (from approximately 38 to 54%) and leukocytes (from 3133 to 4933 per mm3). The results indicated a decline in the levels of antioxidants, i.e. SOD (SOD from approximately 13 to 10 U mg-1 protein and CAT activity from 23 to 9 U mg-1 protein. To the best of our knowledge, this is the first report on the response of Pelibuey sheep to short-term water shortage stress under Cuban environmental conditions.