bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hidden diversity of the most basal tapeworms (Cestoda, Gyrocotylidea), the enigmatic parasites of holocephalans (Chimaeriformes)
Authors: Barčák Daniel , Fan Chia-Kwung , Sonko Pasaikou , Kuchta Roman , Scholz Tomáš , Orosová Martina , Chen Hsuan-Wien , Oros Mikuláš 
Published: March 2021

AbstractGyrocotylideans are evolutionary ancient parasitic flatworms, and like their hosts—a relict group of holocephalan fishes (Chimaeriformes)—they are considered to be “living fossils” of a vanished past. However, the species diversity, host associations and biogeography of these most basal tapeworms are poorly known. Herein, we provide evidence of a conspicuous contrast between the genetic and morphological data based on an examination of newly collected and properly processed Gyrocotyle specimens (hologenophores) isolated from holocephalans off Taiwan and Argentina. Our molecular data, inferred from three genes (COI, 28S rRNA, 18S rRNA), showed unexpected genetic interrelationships among isolates of the genus Gyrocotyle, because each of the four genotypes from Taiwan clustered with isolates of distinct gyrocotylideans from the North Atlantic. Three genotypes of Gyrocotyle from Taiwan were morphologically almost indistinguishable from each other but represented distinct genetic lineages; a single specimen of Gyrocotyle sp. genotype 4 exhibited a clear genetic and morphological distinctness, though its formal description as a new species would be premature. Additionally, specimens of Gyrocotyle rugosa Diesing, 1850, from the type host Callorhinchus callorynchus from Argentina, provided the first genetic data on the type species of the genus and enabled us to characterise it, which is necessary for future taxonomic studies. The finding of some specimens of Gyrocotyle sp. genotype 3 in Chimaera phantasma, and another one in C. cf. argiloba, together with the putative conspecificity of an unidentified gyrocotylidean from Callorhinchus milii off Australia and G. rugosa from C. callorynchus off Argentina, represent evidence that one gyrocotylidean species may parasitise more than one holocephalan host species. Existing taxonomic problems and conflicts between morphological and molecular data on species of Gyrocotyle can only be resolved if hologenophores from type hosts and localities of nominal taxa are properly characterised genetically and morphologically.
Amplification of potential thermogenetic mechanisms in cetacean brains compared to artiodactyl brains
Authors: Manger Paul R. , Patzke Nina , Spocter Muhammad A. , Bhagwandin Adhil , Karlsson Karl Æ. , Bertelsen Mads F. , Alagaili Abdulaziz N. , Bennett Nigel C. , Mohammed Osama B. , Herculano-Houzel Suzana , Hof Patrick R. , Fuxe Kjell 
Published: March 2021

AbstractTo elucidate factors underlying the evolution of large brains in cetaceans, we examined 16 brains from 14 cetartiodactyl species, with immunohistochemical techniques, for evidence of non-shivering thermogenesis. We show that, in comparison to the 11 artiodactyl brains studied (from 11 species), the 5 cetacean brains (from 3 species), exhibit an expanded expression of uncoupling protein 1 (UCP1, UCPs being mitochondrial inner membrane proteins that dissipate the proton gradient to generate heat) in cortical neurons, immunolocalization of UCP4 within a substantial proportion of glia throughout the brain, and an increased density of noradrenergic axonal boutons (noradrenaline functioning to control concentrations of and activate UCPs). Thus, cetacean brains studied possess multiple characteristics indicative of intensified thermogenetic functionality that can be related to their current and historical obligatory aquatic niche. These findings necessitate reassessment of our concepts regarding the reasons for large brain evolution and associated functional capacities in cetaceans.
Cyproterone acetate acts as a disruptor of the aryl hydrocarbon receptor
Authors: Chen Chih-Shou , Gao Guan-Lun , Ho Dong-Ru , Lin Chih-Yi , Chou Yu-Ting , Chen Shan-Chun , Huang Min-Cong , Kao Wen-Ya , Su Jyan-Gwo Joseph 
Published: March 2021

AbstractProstate cancer is a major cause of death in males. Cyproterone acetate (CPA), the steroidal anti-androgen for part of androgen deprivation therapy, may block the androgen-receptor interaction and then reduce serum testosterone through its weak anti-gonadotropic action. In addition to CPA inducing hepatitis, CPA is known to cause liver tumors in rats also. Aryl hydrocarbon receptor (AhR) is a cytoplasmic receptor and regulates multiple physiological functions. CYP1A1 is an AhR-targeted gene. We found that CPA induced CYP1A1 expression, transcriptional activity of the aryl hydrocarbon response element (AHRE), and the nuclear localization of AhR in mouse Hepa-1c1c7 cells. However, CPA suppressed CYP1A1 mRNA expression and the transcriptional activity of AHRE in human HepG2 and MCF7 cells, and also decreased AhR ligand-induced CYP1A1 protein expression and transcriptional activity of AHRE in HepG2 cells. In summary, CPA is an AhR agonist in mouse cells, but an AhR antagonist in human cells. Accordingly, CPA potentially plays a role as an endocrine disruptor of the AhR. This study helps us to understand why CPA induces acute hepatitis, gene mutation, and many other side effects. In addition, it may trigger further studies investigating the relationships between CPA, glucocorticoid receptor and castration-resistant prostate cancer in the future.
Field-deployable, rapid diagnostic testing of saliva for SARS-CoV-2
Authors: Wei Shan , Suryawanshi Hemant , Djandji Alexandre , Kohl Esther , Morgan Stephanie , Hod Eldad A. , Whittier Susan , Roth Kevin , Yeh Raymond , Alejaldre Juan Carlos , Fleck Elaine , Ferrara Stephen , Hercz Daniel , Andrews David , Lee Lilly , Hendershot Kristopher A. , Goldstein Joshua , Suh Yousin , Mansukhani Mahesh , Williams Zev 
Published: March 2021

AbstractTo safely re-open economies and prevent future outbreaks, rapid, frequent, point-of-need, SARS-CoV-2 diagnostic testing is necessary. However, existing field-deployable COVID-19 testing methods require the use of uncomfortable swabs and trained providers in PPE, while saliva-based methods must be transported to high complexity laboratories for testing. Here, we report the development and clinical validation of High-Performance Loop-mediated isothermal Amplification (HP-LAMP), a rapid, saliva-based, SARS-CoV-2 test with a limit of detection of 1.4 copies of virus per µl of saliva and a sensitivity and specificity with clinical samples of > 96%, on par with traditional RT-PCR based methods using swabs, but can deliver results using only a single fluid transfer step and simple heat block. Testing of 120 patient samples in 40 pools comprised of 5 patient samples each with either all negative or a single positive patient sample was 100% accurate. Thus, HP-LAMP may enable rapid and accurate results in the field using saliva, without need of a high-complexity laboratory.
Hydrological droughts in the southern Andes (40–45°S) from an ensemble experiment using CMIP5 and CMIP6 models
Authors: Aguayo Rodrigo , León-Muñoz Jorge , Garreaud René , Montecinos Aldo 
Published: March 2021

AbstractThe decrease in freshwater input to the coastal system of the Southern Andes (40–45°S) during the last decades has altered the physicochemical characteristics of the coastal water column, causing significant environmental, social and economic consequences. Considering these impacts, the objectives were to analyze historical severe droughts and their climate drivers, and to evaluate the hydrological impacts of climate change in the intermediate future (2040–2070). Hydrological modelling was performed in the Puelo River basin (41°S) using the Water Evaluation and Planning (WEAP) model. The hydrological response and its uncertainty were compared using different combinations of CMIP projects (n = 2), climate models (n = 5), scenarios (n = 3) and univariate statistical downscaling methods (n = 3). The 90 scenarios projected increases in the duration, hydrological deficit and frequency of severe droughts of varying duration (1 to 6 months). The three downscaling methodologies converged to similar results, with no significant differences between them. In contrast, the hydroclimatic projections obtained with the CMIP6 and CMIP5 models found significant climatic (greater trends in summer and autumn) and hydrological (longer droughts) differences. It is recommended that future climate impact assessments adapt the new simulations as more CMIP6 models become available.
Gradual onset of the Maunder Minimum revealed by high-precision carbon-14 analyses
Authors: Miyahara Hiroko , Tokanai Fuyuki , Moriya Toru , Takeyama Mirei , Sakurai Hirohisa , Horiuchi Kazuho , Hotta Hideyuki 
Published: March 2021

AbstractThe Sun exhibits centennial-scale activity variations and sometimes encounters grand solar minimum when solar activity becomes extremely weak and sunspots disappear for several decades. Such an extreme weakening of solar activity could cause severe climate, causing massive reductions in crop yields in some regions. During the past decade, the Sun’s activity has tended to decline, raising concerns that the Sun might be heading for the next grand minimum. However, we still have an underdeveloped understanding of solar dynamo mechanisms and hence precise prediction of near-future solar activity is not attained. Here we show that the 11-year solar cycles were significantly lengthened before the onset of the Maunder Minimum (1645–1715 CE) based on unprecedentedly high-precision data of carbon-14 content in tree rings. It implies that flow speed in the convection zone is an essential parameter to determine long-term solar activity variations. We find that a 16 year-long cycle had occurred three solar cycles before the onset of prolonged sunspot disappearance, suggesting a longer-than-expected preparatory period for the grand minimum. As the Sun has shown a tendency of cycle lengthening since Solar Cycle 23 (1996–2008 CE), the behavior of Solar Cycle 25 can be critically important to the later solar activity.
DropConnect is effective in modeling uncertainty of Bayesian deep networks
Authors: Mobiny Aryan , Yuan Pengyu , Moulik Supratik K. , Garg Naveen , Wu Carol C. , Van Nguyen Hien 
Published: March 2021

AbstractDeep neural networks (DNNs) have achieved state-of-the-art performance in many important domains, including medical diagnosis, security, and autonomous driving. In domains where safety is highly critical, an erroneous decision can result in serious consequences. While a perfect prediction accuracy is not always achievable, recent work on Bayesian deep networks shows that it is possible to know when DNNs are more likely to make mistakes. Knowing what DNNs do not know is desirable to increase the safety of deep learning technology in sensitive applications; Bayesian neural networks attempt to address this challenge. Traditional approaches are computationally intractable and do not scale well to large, complex neural network architectures. In this paper, we develop a theoretical framework to approximate Bayesian inference for DNNs by imposing a Bernoulli distribution on the model weights. This method called Monte Carlo DropConnect (MC-DropConnect) gives us a tool to represent the model uncertainty with little change in the overall model structure or computational cost. We extensively validate the proposed algorithm on multiple network architectures and datasets for classification and semantic segmentation tasks. We also propose new metrics to quantify uncertainty estimates. This enables an objective comparison between MC-DropConnect and prior approaches. Our empirical results demonstrate that the proposed framework yields significant improvement in both prediction accuracy and uncertainty estimation quality compared to the state of the art.
The less conserved metal-binding site in human CRISP1 remains sensitive to zinc ions to permit protein oligomerization
Authors: Sheng Jie , Gadella Bart M. , Olrichs Nick K. , Kaloyanova Dora V. , Helms J. Bernd 
Published: March 2021

AbstractCysteine-rich secretory proteins (CRISPs) are a subgroup of the CRISP, antigen 5 and PR-1 (CAP) superfamily that is characterized by the presence of a conserved CAP domain. Two conserved histidines in the CAP domain are proposed to function as a Zn2+-binding site with unknown function. Human CRISP1 is, however, one of the few family members that lack one of these characteristic histidine residues. The Zn2+-dependent oligomerization properties of human CRISP1 were investigated using a maltose-binding protein (MBP)-tagging approach in combination with low expression levels in XL-1 Blue bacteria. Moderate yields of soluble recombinant MBP-tagged human CRISP1 (MBP-CRISP1) and the MBP-tagged CAP domain of CRISP1 (MBP-CRISP1ΔC) were obtained. Zn2+specifically induced oligomerization of both MBP-CRISP1 and MBP-CRISP1ΔCin vitro. The conserved His142 in the CAP domain was essential for this Zn2+dependent oligomerization process, confirming a role of the CAP metal-binding site in the interaction with Zn2+. Furthermore, MBP-CRISP1 and MBP-CRISP1ΔColigomers dissociated into monomers upon Zn2+removal by EDTA. Condensation of proteins is characteristic for maturing sperm in the epididymis and this process was previously found to be Zn2+-dependent. The Zn2+-induced oligomerization of human recombinant CRISP1 may shed novel insights into the formation of functional protein complexes involved in mammalian fertilization.
Space evaluation in football games via field weighting based on tracking data
Authors: Narizuka Takuma , Yamazaki Yoshihiro , Takizawa Kenta 
Published: March 2021

AbstractIn football game analysis, space evaluation is an important issue because it is directly related to the quality of ball passing or player formations. Previous studies have primarily focused on a field division approach wherein a field is divided into dominant regions in which a certain player can arrive prior to any other players. However, the field division approach is oversimplified because all locations within a region are regarded as uniform herein. The objective of the current study is to propose a fundamental framework for space evaluation based on field weighting. In particular, we employed the motion model and calculated a minimum arrival time $$ \tau $$
τ
for each player to all locations on the football field. Our main contribution is that two variables $$ \tau _{\text{of}} $$

τ
of

and $$ \tau _{\text{df}} $$

τ
df

corresponding to the minimum arrival time for offense and defense teams are considered; using $$ \tau _{\text{of}} $$

τ
of

and $$ \tau _{\text{df}} $$

τ
df

, new orthogonal variables $$ z_{1} $$

z
1

and $$ z_{2} $$

z
2

are defined. In particular, based on real datasets comprising of data from 45 football games of the J1 League in 2018, we provide a detailed characterization of $$ z_{1} $$

z
1

and $$ z_{2} $$

z
2

in terms of ball passing. By using our method, we found that $$ z_{1}(\vec {x}, t) $$

z
1

(

x
→

,
t
)

and $$ z_{2}(\vec {x}, t) $$

z
2

(

x
→

,
t
)

represent the degree of safety for a pass made to $$ \vec {x} $$

x
→

at t and degree of sparsity of $$ \vec {x} $$

x
→

at t, respectively; the success probability of passes could be well-fitted using a sigmoid function. Moreover, a new type of field division approach and evaluation of ball passing just before shots using real game data are discussed.
The relationship between Subfoveal Choroidal Thickness and Hypertensive Retinopathy
Authors: Shao Lei , Zhou Ling Xiao , Xu Liang , Wei Wen Bin 
Published: March 2021

AbstractThe Beijing Eye Study 2011 is a population-based cross-sectional study in Northern China, which enrolled 3468 participants whose age were more than 50 years. A detailed ophthalmic examination was performed including spectral-domain optical coherence tomography with enhanced depth imaging for measurement of SFCT and fundus photography. Blood pressure, fundus photographs and choroidal OCT-images were available for 3237 (93.3%) subjects, with 1953 (56.3 ± 0.8%) of the study population fulfilled the diagnosis of hypertension and 1089 subjects having hypertensive retinopathy. For the hypertensive cases, the SFCT in patients with hypertensive retinopathy (286.48 ± 105.23 µm) was significantly thicker than subjects without hypertensive retinopathy (187.04 ± 78.80 µm, P < 0.001). SFCT was significantly associated with the stage of hypertensive retinopathy (P < 0.001), but not significantly associated with diastolic blood pressure (P = 0.94), history (P = 0.95) and years (P = 0.91) of hypertension. In conclusion, hypertension as systemic disease was not significantly affect the subfoveal choroidal thickness, but as ocular disease, hypertensive retinopathy was significantly related to changes of choroidal thickness. Lesions of choroid during chronic hypertension may play an important role in development of hypertensive retinopathy.
Bumble bee queens activate dopamine production and gene expression in nutritional signaling pathways in the brain
Authors: Sasaki Ken , Yokoi Kakeru , Toga Kouhei 
Published: March 2021

AbstractTo explore the neuroendocrine mechanisms underlying caste-specific behavior and its evolution from primitive to advanced eusocial bees, the monoamine levels and expression of genes involved in monoamine production and signaling in the brain were compared between the castes of Bombus ignitus. Higher levels of dopamine and its related substances were found in the brains of newly emerged queens than in the brains of emerged workers. The degree of caste differences in B. ignitus was smaller than that reported in Apis mellifera, indicating a link to different social stages in the two species. There was no differential expression in genes involved in dopamine biosynthesis between castes, suggesting that the high dopamine production in queens was not largely influenced by the expression of these genes at emergence, rather it might be influenced by tyrosine supply. Genome-wide analyses of gene expression by RNA-sequencing indicated that a greater number of genes involved in nutrition were actively expressed in the brains of newly emerged queens in comparison to the emerged workers. Some of the expression was confirmed by real-time quantitative PCR. The signaling pathways driven by the expression of these genes may be associated with dopamine signaling or the parallel activation of dopamine production.
Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
Authors: Gonzalez-Galofre Zaniah N. , Alcaide-Corral Carlos J. , Tavares Adriana A. S. 
Published: March 2021

Abstract18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in small animal research intravenous administrations can be challenging, because partial paravenous injection is common due to the small calibre of the superficial tail veins and repeat administrations via tail veins can lead to tissue injury therefore limiting the total number of longitudinal scanning points. In this paper, the feasibility of using intra-peritoneal route of injection of 8F-NaF to study calcification in mice was studied by looking at the kinetic and uptake profiles of normal soft tissues and bones versus intra-vascular injections. Dynamic PET was performed for 60 min on nineteen isoflurane-anesthetized male Swiss mice after femoral artery (n = 7), femoral vein (n = 6) or intraperitoneal (n = 6) injection of 8F-NaF. PET data were reconstructed and the standardised uptake value (SUV) and standardised uptake value ratio (SUVr) were estimated from the last three frames between 45- and 60-min and 8F-NaF uptake constant (Ki) was derived by Patlak graphical analysis. In soft tissue, the 18F-NaF perfusion phase changes depending on the type on injection route, whereas the uptake phase is similar regardless of the administration route. In bone tissue SUV, SUVr and Ki measures were not significantly different between the three administration routes. Comparison between PET and CT measures showed that bones that had the highest CT density displayed the lowest PET activity and conversely, bones where CT units were low had high 8F-NaF uptake. Intraperitoneal injection is a valid and practical alternative to the intra-vascular injections in small-animal 18F-NaF PET imaging providing equivalent pharmacokinetic data. CT outcome measures report on sites of stablished calcification whereas PET measures sites of higher complexity and active calcification.
Amide proton transfer weighted (APTw) imaging based radiomics allows for the differentiation of gliomas from metastases
Authors: Sartoretti Elisabeth , Sartoretti Thomas , Wyss Michael , Reischauer Carolin , van Smoorenburg Luuk , Binkert Christoph A. , Sartoretti-Schefer Sabine , Mannil Manoj 
Published: March 2021

AbstractWe sought to evaluate the utility of radiomics for Amide Proton Transfer weighted (APTw) imaging by assessing its value in differentiating brain metastases from high- and low grade glial brain tumors. We retrospectively identified 48 treatment-naïve patients (10 WHO grade 2, 1 WHO grade 3, 10 WHO grade 4 primary glial brain tumors and 27 metastases) with either primary glial brain tumors or metastases who had undergone APTw MR imaging. After image analysis with radiomics feature extraction and post-processing, machine learning algorithms (multilayer perceptron machine learning algorithm; random forest classifier) with stratified tenfold cross validation were trained on features and were used to differentiate the brain neoplasms. The multilayer perceptron achieved an AUC of 0.836 (receiver operating characteristic curve) in differentiating primary glial brain tumors from metastases. The random forest classifier achieved an AUC of 0.868 in differentiating WHO grade 4 from WHO grade 2/3 primary glial brain tumors. For the differentiation of WHO grade 4 tumors from grade 2/3 tumors and metastases an average AUC of 0.797 was achieved. Our results indicate that the use of radiomics for APTw imaging is feasible and the differentiation of primary glial brain tumors from metastases is achievable with a high degree of accuracy.
The medicinal plant Tabebuia impetiginosa potently reduces pro-inflammatory cytokine responses in primary human lymphocytes
Authors: Ryan Rachael Y. M. , Fernandez Alejandra , Wong Yide , Miles John J. , Cock Ian E. 
Published: March 2021

AbstractBark from theHandroanthus impetiginosus(Mart. ex DC.) Mattos (Bignoniaceae) tree has long been used in traditional South American healing practises to treat inflammation. However, its anti-inflammatory activity has not been closely examined. Here we use chemical extraction, qualitative phytochemical examination, toxicity testing and quantitative examination of anti-inflammatory activity on human cells ex vivo. All extracts were found to be nontoxic. We found different extracts exhibited unique cytokine profiles with some extracts outperforming a positive control used in the clinic. These results verify the immunomodulatory activity ofHandroanthus impetiginosus(Mart. ex DC.) Mattos (Bignoniaceae) tree bark-derived compounds. Collectively, combining a lack of toxicity and potency in human immune cells supports further fractionation and research.