bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peningkatan Dukungan Keluarga dalam Perawatan Klien Tuberkulosis melalui Terapi Psikoedukasi
Authors: Jauhar Muhamad , Darmawati Laesa , Wijayati Sugih , Surahmi Fajar 
Published: December 2024

ENGLISHAs many as 1.02 million cases of tuberculosis have been reported in poor nations. Non-compliance with prescribed treatments is one of the causes. The result is the spread of disease among the family and community. The family plays a crucial part in addressing the issue since they act as a drug monitor. Psychoeducational therapy is the intervention that can be given to the family. The purpose is to determine how psychoeducation treatment affects family support for treating TB patients. The study included a control group and a quasi-experimental pre-post-test approach. From February to March 2020, the study was carried out at the Public Health Center Semarang Regency. Purposive sampling was used to choose the 28-person sample that was used for each group. The tool employed had a reliability rating of 0.698 and was a questionnaire about family assistance for tuberculosis patients. Both paired and independent t-tests were employed in the data analysis. With p = 0.000 (p 0.05), psycho-educational therapy greatly influences families' willingness to help TB patients. The family that provided this therapy can become better at supporting the patient during treatment. To improve tuberculosis drug adherence, psychoeducation therapy can be incorporated with the DOTS program at all healthcare facilities. INDONESIATren peningkatan kasus tuberkulosis terlihat di negara-negara berkembang termasuk Indonesia dengan jumlah kasus sebanyak 1,02 juta kasus. Salah satu faktor penyebabnya adalah ketidakpatuhan pengobatan. Dampaknya adalah penularan penyakit dalam keluarga dan masyarakat. Keluarga sebagai pengawas menelan obat memiliki peran penting dalam menyelesaikan masalah tersebut. Salah satu intervensi yang dapat diberikan kepada keluarga adalah terapi psikoedukasi. Penelitian ini mengidentifikasi pengaruh terapi psikoedukasi terhadap dukungan keluarga untuk merawat klien TB. Desain penelitian menggunakan pre-post-test quasi-eksperimental dengan kelompok kontrol. Penelitian dilakukan di Balai Kesehatan Masyarakat Kabupaten Semarang pada bulan Februari sampai Maret 2020. Jumlah sampel 28 untuk setiap kelompok dipilih secara purposive sampling. Instrumen penelitian menggunakan kuesioner dukungan keluarga klien tuberkulosis dengan nilai reliabilitas 0,698. Analisis data menggunakan paired t-test dan independent t-test. Penelitian ini telah lolos uji etik dari Komisi Bioetika Universitas Sultan Agung dengan nomor 094/III/2020/Komisi Bioetika. Terdapat pengaruh terapi psikoedukasi terhadap peningkatan dukungan keluarga untuk merawat klien TB secara signifikan dengan p=0,000 (p<0,05). Keluarga yang telah mendapatkan terapi ini dapat meningkatkan kemampuannya dalam memberikan dukungan selama pengobatan. Terapi psikoedukasi dapat diintegrasikan dengan program DOTS di fasilitas layanan kesehatan untuk meningkatkan kepatuhan terapi obat tuberkulosis.
Long noncoding RNA, PURPL is associated with aneuploidy and its magnitude of expression level is dependent on P53 status
Authors: Malakar Pushkar 
Published: December 2024

IntroductionLong non-coding RNAs (lncRNAs) are a fascinating, but still largely uncharacterized, class of genes. Recently, lncRNAs have attracted significant attention due to their emerging functions in development and disease. The role of lncRNAs in chromosome instability or aneuploidy is not extensively studied.MethodsWe started with the objective of characterizing lncRNAs that play an important role in chromosome instability (CIN) or aneuploidy. Here, we report the initial functional characterization of PURPL in the context of chromosomal instability or aneuploidy.ResultsWe report the over-expression of lncRNA PURPL in three experimental models of chromosomal instability, or aneuploidy. In addition, the study also showed that the extent or magnitude of PURPL expression is dependent upon p53 status. Our research also showed that turning off PURPL is enough to create a CIN phenotype in RPE-1 cell lines that were previously karyotypically stable. Moreover, PURPL knockdown cells are more sensitive to CIN or aneuploidy inducers.DiscussionThese findings show that PURPL is essential for maintaining chromosomal or genomic stability in mammalian cells. Collectively, the study demonstrated that lncRNA-PURPL significantly contributes to CIN, or aneuploidy.
Causality of genetically determined glucosamine supplementation on cognition and sarcopenia: a Mendelian randomization study
Authors: Kang Yi , Tang Yidan , Kong Weishuang , Zhu Tao , Chen Guo 
Published: December 2024

BackgroundEvidence indicates a negative link between glucosamine and age-related cognitive decline and sarcopenia. However, the causal relationship remains uncertain. This study aims to verify whether glucosamine is causally associated with cognitive function and sarcopenia.MethodForty-eight genetic variants linked to glucosamine use were extracted from the MRC-IEU consortium. Besides, we gathered cognition proxy indicators [cognitive performance and fluid intelligence score (FIS)], and sarcopenia-related indicators, namely, appendicular lean mass (ALM), whole body fat-free mass (WBFM), low hand grip strength, facial aging (FA), moderate to vigorous physical activity levels, usual walking pace and DNA methylation GrimAge acceleration from the large publicly available genome-wide association studies. Initially, Mendelian randomization (MR) analyses were conducted to ascertain the causal impact of glucosamine on cognition and sarcopenia-related traits. Subsequently, the two-step MR and multivariable MR (MVMR) were employed to examine whether any mediators causally mediated the observed associations.ResultMR analysis indicated that glucosamine was associated with increased cognitive performance (p = 8.46E-04), FIS (p = 7.50E-04), ALM (p = 6.45E-08), WBFM (p = 1.97E-03), usual walking pace (p = 2.55E-07), and moderate to vigorous physical activity levels (p = 3.29E-03), but associated with decreased FA risk (p = 3.77E-05) and DNA methylation GrimAge acceleration (p = 0.001). However, there were no significant causal associations between glucosamine and low hand grip strength. Multivariable MR showed that glucosamine continued to have a significant effect on cognitive performance, FIS, ALM, WBFM, usual walking pace, and moderate to vigorous physical activity levels after controlling for osteoarthritis (OA) and body mass index (BMI) (p &lt; 0.05). We further found that C-reactive protein levels (CRP) may mediate the association of glucosamine and ALM, WBFM, usual walking pace, and physical activity (p &lt; 0.05), and basal metabolic rate (BMR) may mediate the association of glucosamine and cognitive performance, FIS, ALM, WBFM, and usual walking pace (p &lt; 0.05).ConclusionRegular glucosamine use enhances cognitive function and postpones sarcopenia for preserving the functional capacities necessary, and the impact of glucosamine on cognition and sarcopenia could be partially attributed to the mediation of BMR and CRP.
Gender and activity disparities in the relationship between circadian syndrome and gallstone disease
Authors: Feng Binbin , Wang Tianlin , Han Junquan , Yang Zhaoshuai , Wang Hong , Li Huizhen 
Published: December 2024

ObjectiveTo explore the potential correlation between gallstone disease (GSD) prevalence and circadian syndrome (CircS).MethodsThe cross-sectional research utilized data spanning 2017 to March 2020, sourced from the National Health and Nutrition Examination Survey (NHANES). The GSD data were collected via questionnaires, with appropriate sample weights applied to ensure the study population was representative. Three multivariable logistic regression models were built to clarify the connection between CircS and GSD. Furthermore, subgroup analysis and interaction test were carried out, categorized based on demographic traits and lifestyle aspects, to discern the potential influence of these variables on the correlation.ResultsThe analysis included 4,126 participants, with a prevalence of 38.68% for CircS and 12.04% for GSD. The multivariable logistic regression analysis indicated a positive correlation between CircS and the prevalence of GSD (Odds Ratio (OR) = 1.336, 95% Confidence Interval (CI): 1.048, 1.702). When stratified by the number of CircS components, a positive correlation was observed between the number of CircS components and the prevalence of GSD (P for trend &lt; 0.05). In particular, individuals with six or more CircS components had a higher prevalence of GSD than those with three or fewer components (OR = 2.608, 95% CI: 1.464, 4.647). The subgroup analysis and interaction test revealed that a positive correlation between CircS and GSD prevalence was mainly observed in female individuals (OR = 1.701, 95% CI: 1.236, 2.341) and individuals not engaged in moderate activity (OR = 1.990, 95% CI: 1.158, 3.418).ConclusionThere is a positive correlation between CircS and GSD prevalence, particularly among females and individuals not engaging in moderate activity. These findings offer new insights for research directions in GSD and may impact preventive and therapeutic strategies.
The systemic inflammation response index as a significant predictor of short-term adverse outcomes in acute decompensated heart failure patients: a cohort study from Southern China
Authors: Xie Lin , Wang Qun , Lu Hengcheng , Kuang Maobin , He Shiming , Xie Guobo , Sheng Guotai , Zhang Shuhua , Wang Wei , Zou Yang 
Published: December 2024

ObjectiveThe deterioration of acute decompensated heart failure (ADHF) is associated with abnormal activation of inflammatory pathways. This study aims to evaluate the impact and predictive value of a novel inflammatory marker, the systemic inflammation response index (SIRI), on short-term adverse outcomes in ADHF patients.MethodsThis retrospective cohort study included 1,448 ADHF patients from Jiangxi Provincial People’s Hospital between 2019-2022. SIRI was calculated using the formula: (neutrophil count × monocyte count)/lymphocyte count. In the correlation analysis, the study outcome was the 30-day mortality in patients with ADHF. Cox regression analysis and receiver operating characteristic curves were employed to investigate the risk assessment and predictive value of the SIRI for 30-day mortality in ADHF patients. Finally, we also exploratively assessed the mediation effect of nutritional factors (albumin: Alb, total cholesterol: TC, and lymphocyte count) on the association between SIRI and 30-day mortality in ADHF patients.ResultsDuring the 30-day follow-up, 53 deaths were recorded. Mortality rates across SIRI tertiles were 0.62%, 2.07%, and 8.28%, respectively. There was a significant linear positive correlation between SIRI and 30-day mortality in ADHF patients (HR: 1.21; P for non-linearity = 0.113). Additionally, compared to ADHF patients with low SIRI, those with high SIRI had a 685% increased risk of 30-day mortality (HR: 7.85). Furthermore, receiver operating characteristic curve analysis demonstrated that SIRI significantly improved the predictive value for 30-day mortality in ADHF patients compared to neutrophil count, monocyte count, and lymphocyte count alone (AUC: neutrophil count 0.7633, monocyte count 0.6835, lymphocyte count 0.7356, SIRI 0.8237; all DeLong P&lt;0.05). Mediation analyses indicated that, except for lymphocyte count, both Alb and TC had significant indirect effects on the SIRI-related 30-day mortality in ADHF patients; Specifically, Alb accounted for approximately 24.46% of the mediation effect, while TC accounted for approximately 13.35%.ConclusionThis cohort study based on a Southern Chinese population demonstrates a significant linear positive correlation between SIRI and 30-day mortality in ADHF patients, highlighting its substantial predictive value. Incorporating SIRI into the monitoring regimen of ADHF patients may be crucial for preventing further disease progression.
Association between SOFA score and risk of acute kidney injury in patients with diabetic ketoacidosis: an analysis of the MIMIC-IV database
Authors: Hua Yiming , Ding Ning , Jing Huaizhi , Xie Yifei , Wu Hao , Wu Yue , Lan Beidi 
Published: December 2024

IntroductionThe Sequential Organ Failure Assessment (SOFA) score is a widely utilized clinical tool for evaluating the severity of organ failure in critically ill patients and assessing their condition and prognosis in the intensive care unit (ICU). Research has demonstrated that higher SOFA scores are associated with poorer outcomes in these patients. However, the predictive value of the SOFA score for acute kidney injury (AKI), a common complication of diabetic ketoacidosis (DKA), remains uncertain. Therefore, this study aims to investigate the relationship between SOFA scores and the incidence of AKI in patients with DKA.MethodsThe study population was divided into two groups based on the median SOFA score (Q1: SOFA ≤3; Q2: SOFA &gt;3). The primary endpoint was the incidence of AKI in patients with DKA. Secondary endpoints included renal replacement therapy (RRT) utilization and in-hospital mortality. Kaplan–Meier survival analysis, Cox proportional hazards models, and logistic regression models were employed to assess the association between SOFA and therisk of AKI in patients with DKA.ResultsOverall, 626 patients with DKA were included in this study, of which 335 (53%) were male. Kaplan–Meier survival analysis included that patients with higher SOFA scores experienced significantly increased cumulative incidences of AKI, higher rates of RRT utilization, and elevated in-hospital mortality. Furthermore, after adjusting for confounding factors, logistic regression and Cox proportional hazards analyses confirmed that SOFA scores remained significantly associated with the incidence of AKI in patients with DKA.ConclusionsOur study indicates that a high SOFA score is an independent risk predictor for the occurrence of AKI, the utilization of RRT, and in-hospital mortality in patients with DKA. The sofa score can be utilized as a biomarker to assess the risk of AKI in this patient population.
Contrast-enhanced ultrasound as a valuable tool to detect minimal inflammation in RA patients in sustained remission
Authors: Polido-Pereira Joaquim , António Manuel S. , Khmelinskii Nikita , Arese Marta , Teixeira Rui , Vieira-Sousa Elsa , D'Agostino Maria A. , Fonseca João E. 
Published: December 2024

ObjectiveThe study aimed to explore the utility of contrast-enhanced ultrasound (CEUS) as a tool for detecting minimal inflammation in rheumatoid arthritis (RA) patients in sustained remission (SR) and to correlate the findings with Disease Activity Score 28 (DAS28) status scores and various ultrasound (US) scores.Patients and methodsThirty RA patients in SR (minimum 6 months), 12 with active disease, and 10 healthy controls were included. Clinical evaluations and US assessments were performed, including grayscale US (GSUS), power Doppler US (PDUS), and Global OMERACT-EULAR Synovitis Score (GLOESS). The CEUS was performed in the two most active joints and was scored semi-quantitatively (SQ) and quantitatively.ResultsHealthy controls and remission RA patients had similar total US scores. Active RA patients had higher US scores than the healthy and remission groups, with statistically significant differences in all the groups compared to the healthy group. However, significant differences were only observed in the GSUS and GLOESS when comparing active RA patients with the remission group. Ninety-five joints were selected for the CEUS, and we detected more microvascularization with the SQ CEUS score than with the PDUS in all groups (18 vs. 58% in the remission group; p-value 0.006). The weighted Cohen's kappa for the intra-rater and inter-rater IACUS CEUS score was 0.714 (confidence interval 0.610–0.819, p-value &lt; 0.001) and 0.540 (confidence interval: 0.419–0.662, p-value &lt; 0.001), respectively. Spearman's correlation between the SQ CEUS and quantitative scores was 0.655.ConclusionFor the majority of RA patients in SR, conventional US may fail to detect microvascularization potentially related to the subclinical disease. The CEUS may be helpful for this purpose.
Antimicrobial activity of nanostructured lipid carriers loaded with Melaleuca armillaris essential oil against Staphylococcus aureus sensitive and resistant to methicillin
Authors: Buldain Daniel , Gortari Castillo Lihuel , Marchetti Laura , Buchamer Andrea , Honor Irala Federico , Islan German , Mestorino Nora 
Published: December 2024

IntroductionBovine mastitis is a major infectious disease affecting dairy cattle, impacting public health and milk industry profitability. Staphylococcus aureus is a contagious pathogen responsible for causing bovine subclinical mastitis. Its pathogenesis and antimicrobial resistance highlight the need for alternative treatments, being the nanoencapsulation of essential oils (EO) very promising.MethodsNanostructured lipid carriers (NLCs) containing 5% of Melaleuca armillaris EO were synthesized and characterized. Their physicochemical characteristics, antimicrobial activity against methicillin-resistant and sensitive S. aureus (MRSA and MSSA, respectively), and protective activity against polymorphonuclear cells were evaluated.ResultsNLC-EO nanoparticles were morphologically spherical and the mean size was around 190 nm, Polydispersity index (PdI) was 0.21 (±0.01), Z potential was −18.4 (±0.4) and EO encapsulation efficiency was 71.5%. Of this parameters Z potential was the only which changed after 6 months of storage at four°C, turning into a more negative value of −31.6 (±1.9). NLC-EO showed a biphasic behavior with a fast initial release during the first 6 h, followed by a slow phase for at least 72 h. Free and nanoencapsulated EO had a minimum inhibitory concentration (MIC90) of 6.25 μL/mL; however, free EO had a minimum inhibitory concentrations of biofilm formation (MICB90) of 3.12 μL/mL and for EO nanoencapsulated was 6.25 μL/mL. Minimum inhibitory concentrations of biofilm formation and eradication (MECB90) were 12.5 μL/mL and 6.25 μL/mL for the nanoencapsulated EO and free EO, respectively. Empty NLC inhibited biofilm formation, but not planktonic growth or eradicated preformed biofilms.DiscussionThe EO was efficiently encapsulated and released from NLC, and its antimicrobial activity against MRSA and MSSA was high. Neutrophil viability was higher when EO was encapsulated, being an important result for future experiments evaluating intracellular EO activity, where Staphylococcus aureus survives and evades poorly penetrating antibiotics activity.
STZ-induced hyperglycemia differentially influences mitochondrial distribution and morphology in the habenulointerpeduncular circuit
Authors: Jodeiri Farshbaf Mohammad , Matos Taelor A. , Niblo Kristi , Alokam Yacoub , Ables Jessica L. 
Published: December 2024

IntroductionDiabetes is a metabolic disorder of glucose homeostasis that is a significant risk factor for neurodegenerative diseases, such as Alzheimer’s disease, as well as mood disorders, which often precede neurodegenerative conditions. We examined the medial habenulainterpeduncular nucleus (MHb-IPN), as this circuit plays crucial roles in mood regulation, has been linked to the development of diabetes after smoking, and is rich in cholinergic neurons, which are affected in other brain areas in Alzheimer’s disease.MethodsThis study aimed to investigate the impact of streptozotocin (STZ)-induced hyperglycemia, a type 1 diabetes model, on mitochondrial and lipid homeostasis in 4% paraformaldehyde-fixed sections from the MHb and IPN of C57BL/6 J male mice, using a recently developed automated pipeline for mitochondrial analysis in confocal images. We examined different time points after STZ-induced diabetes onset to determine how the brain responded to chronic hyperglycemia, with the limitation that mitochondria and lipids were not examined with respect to cell type or intracellular location.ResultsMitochondrial distribution and morphology differentially responded to hyperglycemia depending on time and brain area. Six weeks after STZ treatment, mitochondria in the ventral MHb and dorsal IPN increased in number and exhibited altered morphology, but no changes were observed in the lateral habenula (LHb) or ventral IPN. Strikingly, mitochondrial numbers returned to normal dynamics at 12 weeks. Both blood glucose level and glycated hemoglobin (HbA1C) correlated with mitochondrial dynamics in ventral MHb, whereas only HbA1C correlated in the IPN. We also examined lipid homeostasis using BODIPY staining for neutral lipids in this model given that diabetes is associated with disrupted lipid homeostasis. BODIPY staining intensity was unchanged in the vMHb of STZ-treated mice but increased in the IPN and VTA and decreased in the LHb at 12 weeks. Interestingly, areas that demonstrated changes in mitochondria had little change in lipid staining and vice versa.DiscussionThis study is the first to describe the specific impacts of diabetes on mitochondria in the MHb-IPN circuit and suggests that the cholinergic MHb is uniquely sensitive to diabetesinduced hyperglycemia. Further studies are needed to understand the functional and behavioral implications of these findings.
U-shaped relationship between serum uric acid and gastric cancer risk: a large prospective cohort study
Authors: Huang Junjun , Mi Ningning , Yang Jingli , Zheng Ya , Yuan Jinqiu , Meng Wenbo 
Published: December 2024

ObjectiveWe conducted this study to investigate the relationship between serum uric acid (SUA) levels and the risk of upper gastrointestinal cancer.MethodsWe conducted a prospective cohort study with 475659 cancer-free participants from the UK Biobank. All subjects were grouped into quartiles, and we used a Cox proportional hazards model to analyze the association between SUA levels and the risk of upper gastrointestinal cancer and explore the potential sex-specific relationship.ResultsOf the 475659 participants, 883 eventually developed upper gastrointestinal cancers over a median follow-up period of 6.7 years. We observed that SUA level was positively correlated with the risk of female oral cancer (hazard ratio Quartile 4 vs Quartile 1 (95% CI): 2.05(1.03,4.06)) and negatively associated with the risk of esophageal cancer in the general population (hazard ratio Quartile 3 vs Quartile 1 (95% CI): 0.65(0.45,0.93)). The risk of gastric cancer in males showed a U-shaped trend, decreasing and then increasing as SUA levels increased (hazard ratio Quartile 3 vs Quartile 1 (95% CI): 0.51(0.32,0.81)). The risk of small intestine cancer in females showed a trend of increasing and then decreasing with increasing SUA levels (hazard ratio Quartile 3 vs Quartile 1 (95% CI): 3.34(1.10,10.13)). Interaction analysis indicated that various factors, such as age, sex, smoking and drinking status, family history of cancer and BMI might play an important role in the relationship between SUA and cancer.ConclusionSUA levels are positively associated with the risk of oral cancer risk in females and negatively associated with the risk of esophageal cancer in the general population. Both low and high SUA levels were associated with increased risk of gastric cancer, supporting a U-shaped association.