bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heteromerization of alkaline-sensitive two-pore domain potassium channels
Authors: Khoubza Lamyaa , Kim Eun-Jin , Chatelain Franck C. , Feliciangeli Sylvain , Kang Dawon , Lesage Florian , Bichet Delphine 
Published: November 2021

AbstractTwo-pore domain (K2P) potassium channels are active as dimers. They produce inhibitory currents regulated by a variety of stimuli. Among them, TALK1, TALK2 and TASK2 form a subfamily of structurally related K2P channels stimulated by extracellular alkalosis. The human genes encoding them are clustered on chromosomal region 6p21. They are expressed in different tissues including the pancreas. By analyzing single cell transcriptomic data, we show that these channels are co-expressed in insulin-secreting pancreatic β cells. By different approaches we show that they form functional heterodimers. Heteromerization of TALK2 with TALK1 or with TASK2 endorses TALK2 with sensitivity to extracellular alkalosis in the physiological range. The association of TASK2 with TALK1 and TALK2 increases their unitary conductance. These results provide a new example of heteromerization in the K2P channel family expanding the range of their potential physiological and pathophysiological roles.
Disassembly of hemidesmosomes promotes tumorigenesis in PTEN-negative prostate cancer by targeting plectin into focal adhesions
Authors: Wenta Tomasz , Schmidt Anette , Zhang Qin , Devarajan Raman , Singh Prateek , Yang Xiayun , Ahtikoski Anne , Vaarala Markku , Wei Gong-Hong , Manninen Aki 
Published: November 2021

AbstractLoss of α6β4-dependent hemidesmosomes has been observed during prostate cancer progression. However, the significance and underlying mechanisms by which aberrant hemidesmosome assembly may modulate tumorigenesis remain elusive. Using an extensive CRISPR/Cas9-mediated genetic engineering approaches in different prostate cancer cell lines combined within vivotumorigenesis studies in mice, bone marrow-on-chip assays and bioinformatics, as well as histological analysis of prostate cancer patient cohorts, we demonstrated that simultaneous loss of PTEN and hemidesmosomes induced several tumorigenic properties including proliferation, migration, resistance to anoikis, apoptosis, and drug treatmentin vitro, and increased metastatic capacityin vivo. Our studies showed that these effects were driven by activation of EGFR/PI3K/Akt and FAK/Src-pathways and were abolished by plectin downregulation. Therefore, dual loss of PTEN and hemidesmosomes may have diagnostic value helping to stratify prostate cancer patients with high risk for development of aggressive disease and highlight plectin as a potential therapeutic target in prostate cancer.
Control of Neutrophil activation through Semaphorin 7A-Plexin C1 is essential for immune defense during pulmonary sepsis
Authors: Granja Tiago , Köhler David , Tang Linyan , Burkhardt Philip , Heck-Swain Ka-Lin , Koeppen Michael , Magunia Harry , Bamberg Maximilian , Konrad Franziska , Ngamsri Kristian , Fuhr Anika , Keller Marius , Haeberle Helene A. , Bakchoul Tamam , Zarbock Alexander , Nieswand Bernhard , Rosenberger Peter 
Published: November 2021

ABSTRACTPulmonary defense mechanisms are critical for host integrity during the early phase of pneumonia and sepsis. These processes are fundamentally dependent on the activation of neutrophils during the early phase of the innate immune response. Recent work has shown that semaphorin 7A (Sema7A) holds significant impact on platelet activation, yet its role in neutrophil migration and function is not well known. We report here that Sema7A binds to neutrophil PlexinC1, increasing integrins and L-selectin on the neutrophil surface. Sema7A-induced neutrophil activation also prompted neutrophil chemotaxis in vitro and the formation of platelet-neutrophil complexes in vivo. We also observed altered adhesion and transmigration of neutrophils in Sema7A-/- animals in the lung. Sema7A-/- animals also showed altered crawling properties of neutrophils. This resulted in increased number of neutrophils in the interstitial space of Sema7A-/- animals but reduced numbers of neutrophils in the alveolar space during pneumonia-induced pulmonary sepsis. This was associated with significantly worse outcome of Sema7A-/- animals in a model of Klebsiella pneumoniae. Furthermore, we were able to show a correlation between serum levels of Sema7A in patients with ARDS and oxygenation levels. Thus, we show here that Sema7A has an immunomodulatory effect though which might influence patient outcome during pulmonary sepsis.SummarySema7A controls pulmonary immune defense
Marketing geographical indication products in the digital age: a holistic perspective
Authors: Bartoli Chiara , Bonetti Enrico , Mattiacci Alberto 
Published: November 2021

PurposeThis paper analyses the impact of digitalization in the marketing of geographic indication (GI) products. Specifically, the objective is to provide a systemic and comprehensive view of marketing issues and challenges arising from evolving digitalization in the agriculture sector.Design/methodology/approachThe authors employed an explorative cognitive mapping technique on a sample of key informants among Italian companies and a consortium of registered food, spirits and wine products.FindingsThis study describes the key concepts dominant in the discourse of informants concerning digitalization and its influence on the GI market. Three clusters and two loops were also identified to explain the relationship among key concepts, which stress changes regarding the relationship with the consumer, product experience and innovation, and the integration between physical and virtual space.Research limitations/implicationsThis is the first attempt to apply cognitive maps to GIs. However, the study does have limitations: it was conducted on a small number of producers and was restricted to only Italy. Another limitation is that interviews were conducted during the COVID-19 pandemic.Practical implicationsThe results suggest important practical implications that stress the need for learning and increased digital competences for the efficient embedding of digital technologies in all business areas, increase product value and innovation, and the need for governance in support of digital transformation.Originality/valueThis work creates the new and valuable literature on food marketing and, specifically, on a changing market environment resulting from digitalization, by providing a holistic overview of digitalization in reference to the marketing of GIs.
Vardhman Envirotech: expanding by making world water positive
Authors: Ghura Amarpreet Singh , Abhishek Abhishek 
Published: November 2021

Complexity academic level
The case provides an opportunity for participants to step into the shoes of Doshi and plan a way to expand Vardhman Envirotech (VE) business by either entering the government segment or by undertaking international market expansion. In doing so, participants should understand the existing strategy by taking into consideration the aspects regarding the manner in which VE’s Injection Recharge Well differs from the existing recharge well solutions. VE lack of experience in government segment, as well as international markets provides an interesting context for the case discussion wherein participants have to examine the positives and challenges of both expansion opportunities and decide the way forward.

Supplementary materials
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Subject code
CSS3: Entrepreneurship.
M-shopping service quality dimensions and their effects on customer trust and loyalty: an empirical study
Authors: Zhang Ruoqing , Jun Minjoon , Palacios Sergio 
Published: November 2021

PurposeThe present study seeks to identify the salient mobile shopping (m-shopping) service quality dimensions as perceived by mobile shoppers (m-shoppers) and examines the linkages between the derived m-shopping service quality dimensions, customer trust and customer loyalty.Design/methodology/approachThe research instrument is developed based upon the mostly validated measures of prior studies. A pretest of the questionnaire is conducted to assess the content validity of the measurement scales. An online survey is used to collect the required data. We employ structural equation modeling (SEM) to analyze data collected from 286 m-shoppers.FindingsWe identify five key m-shopping service quality dimensions: responsiveness, personalization, ease of use, aesthetics and perceived risk based on an extensive review of relevant literature. The SEM results show that all the five m-shopping service quality dimensions significantly impact, directly and/or indirectly, customer loyalty. Moreover, the results show that trust plays a partial mediating role in the effects of responsiveness and personalization on loyalty; a full mediating role in the effects of aesthetics and perceived risk on loyalty and no mediating role in the effect of ease of use on loyalty.Practical implicationsMobile retailers (m-retailers) can use the quality measurement tool developed in this study to detect service quality weaknesses and strengths. Based on their quality assessment, m-retailers can effectively allocate corporate resources to the important service quality attributes uncovered by this study, thereby improving their overall service quality performance and in turn expanding their loyal customer base.Originality/valuePrior studies have demonstrated that service quality and customer trust play a pivotal role in enhancing customer loyalty in both offline and online settings. Unfortunately, no research has empirically examined the relationships between service quality dimensions, trust and loyalty in the context of m-shopping. Therefore, a major contribution of this study is to address this research gap and add knowledge to the limited body of post-adoption m-shopping research.
Research on seismic performance of prefabricated concrete-filled steel tubular frame joints
Authors: Li Shufeng , Zhao Di , Zhou Yating 
Published: November 2021

PurposeConcrete-filled steel tube structures are widely used for their high bearing capacity, good plasticity, good fire resistance and optimal seismic performance. In order to give full play to the advantages of concrete-filled steel tube, this paper proposes a prefabricated concrete-filled steel tube frame joint.Design/methodology/approachThe concrete-filled steel tube column and beam are connected by high-strength bolted end-plate, and the steel bars in the concrete beam are welded vertically with the end-plates through the enlarged pier head. In addition, the finite element software ABAQUS is used numerically to study the seismic performance of the structure.FindingsThe ductility coefficient of the joint is in 1.72–6.82, and greater than 2.26 as a whole. The equivalent viscous damping coefficient of the joint is 0.13–3.03, indicating that the structure has good energy dissipation capacity.Originality/valueThe structure is convenient for construction and overcomes the shortcomings of the previous on-site welding and on-site concrete pouring. The high-strength bolted end-plate connection can effectively transfer the load, and each component can give play to its material characteristics.
Attenuation compensation for wavefield‐separation‐based least‐squares reverse time migration in viscoelastic media
Authors: Zhang Wei , Gao Jinghuai 
Published: November 2021

ABSTRACTElastic least‐squares reverse time migration can image the multicomponent seismic recordings. However, on the one hand, without considering the intrinsic attenuation of the subsurface, it may produce blurred reflectivity images with incorrect positions of reflectors for seismic recordings with strong attenuation. On the other hand, the crosstalk artefacts created by the different wave modes may severely degrade the imaging quality. To alleviate these crosstalk artefacts and compensate for the attenuation effects, we have developed a time‐domain wavefield‐separation‐based least‐squares reverse time migration approach in viscoelastic media, which utilizes the viscoelastic wave equation on the basis of the standard linear solid model to simulate intrinsic subsurface attenuation. The key point of the proposed approach is that we utilize the separated gradient contribution of PP‐ and PS‐wave modes based on the wavefield separation technique in viscoelastic media to construct the P‐wave and S‐wave velocity images, respectively. Unlike the conventional wavefield‐separation‐based least‐squares reverse time migration approach, which generally uses the new stress–velocity equations to formulate, our proposed wavefield separation scheme fully depends on the conventional viscoelastic wave equation and its adjoint wave equation. By introducing the pure P‐wave stress in the forward and adjoint wavefields, the coupled wavefields can be well decomposed, which requires much less computational costs than the wavenumber‐domain wavefield separation scheme. Numerical examples using the layered and Marmousi‐2 models have shown that the proposed approach can improve the migration image quality under geological conditions with strong attenuation where elastic least‐squares reverse time migration may produce blurred and unfocused events. Meanwhile, the proposed least‐squares reverse time migration approach with the wavefield separation scheme has a better convergence rate and produces fewer crosstalk artefacts than that without the wavefield separation scheme.
Environmental stoichiometry mediates phytoplankton diversity effects on communities' resource use efficiency and biomass
Authors: Gerhard Miriam , Schlenker Alexandra , Hillebrand Helmut , Striebel Maren 
Published: November 2021

Abstract

Positive biodiversity–ecosystem functioning (BEF) relationships are predicted to increase in strength when high environmental variability allows for complementarity between resource use strategies in diverse communities. This environmental variability can be represented by spatial or temporal variation in nutrient ratios, but resource use efficiency (RUE) and therefore biomass build‐up of primary producers might be restricted when nutrient ratios are highly imbalanced (i.e. limitation by one nutrient and beyond optimal ratios for growth). Although the linkages between ecosystem functioning, diversity and nutrient availability are theoretically well understood, we lack experimental evidence on how phytoplankton diversity affects resource use and biomass under variable nutrient ratios (N:P ratios).

Combining a mesocosm and a microcosm experiment, we tested diversity effects on ecosystem functioning by exposing a species diversity gradient generated by the loss of rare species in a natural community to different N:P ratios (uniform vs. a gradient). The N:P supply ratio gradient also allowed us to evaluate responses across balanced and imbalanced ratios.

We found that increased species diversity led to increased community RUE when supplied a gradient of N:P ratios; but restricted to the highest diversity level. However, diversity did not affect RUE under uniform nutrient ratios. The overall phytoplankton biomass and carbon:nutrient ratios responses to diversity reflected the patterns detected for RUE. Contrary to theoretical predictions, RUE was maintained under highest N:P supply ratios (extreme phosphorous limitation) suggesting that imbalanced N:P ratios do not necessarily decrease function. Thus, we showed that the nutrient context influences diversity effects on RUE and biomass.

Synthesis. Overall, our results suggest that the effect of rare phytoplankton species losses on community RUE and biomass can be compensated by the persistent species when nutrient ratios are uniform, but leads to decreases in ecosystem functioning under variable nutrient ratios. This work provides a first attempt for testing interactions between the nutrient context (including concentrations and ratios) and the diversity of (natural) communities experimentally, which is conceptually understood but poorly tested for phytoplankton.
Women and Civility in British Politics: Reflections on a Changing Environment
Authors: Peele Gillian 
Published: November 2021

AbstractThe last decade has seen mounting evidence of intimidation in British public life involving threatening behaviour towards and the abuse of public figures, especially MPs and parliamentary candidates. The killings of two MPs (Jo Cox and Sir David Amess) have highlighted the seriousness of this development for the health of British democracy. The article examines the problem using the Committee on Standards in Public Life's 2017 report, Intimidation in Public Life. It explores the possible causes of the decline in civility in British public life, and examines possible remedies for addressing the problem. It argues that the decline of civility and the coarsening of political life reflect a multifaced problem and that, in addition to new measures, such as providing MPs with enhanced security and strengthening protections against online harms, wider long‐term changes to our political culture are necessary to promote civility and tolerance and to foster values that reinforce liberal democracy.
Should a Christian Party Conceive Policies in Line with the Preferential Option for the Poor? Italian Historical Experience, 1946–1993
Authors: Sandonà Luca , Solari Stefano 
Published: November 2021

AbstractItaly was governed by one party from 1946 to 1993: the Christian Democrats (Democrazia Cristiana or DC). Initially, the DC Party adopted policies in the classical and conservative tradition to cope with poverty: agrarian reform, expansive fiscal policies, and social housing. After the Second Vatican Council, which led to growing acceptance of the “preferential option for the poor,” a radical change emerged in the DC Party regarding some social‐democratic policies: the party endorsed universal healthcare and social pensions and assistance. The fundamental idea was the “decommodification” of basic services. In these reforms, there were some good results as well as some failures. There were also difficulties with public finances.
Reversible, regional ST‐segment elevation due to chylothorax
Authors: Brown Sarah H. , Neuss Michael J. , Heimlich J. Brett , Kronenberg Marvin W. 
Published: November 2021

AbstractChylothorax is an uncommon complication of thoracic surgery and, to our knowledge, has never been documented as a cause of dynamic ST‐segment elevation (STE). A 63‐year‐old woman with history of right pneumonectomy presented with chest pain and regional STE on 12‐lead electrocardiogram (ECG). Normal troponin‐I and a computed tomography (CT) scan showing a large right hemithoracic fluid collection indicated the unique cause of STE, which resolved after thoracentesis, was pericardial inflammation and cardiac compression from chylothorax. This case emphasizes nuances of ECG interpretation in the context of regional STE and explores the pathophysiology that links chylothorax with acute pericarditis.
Aptamer‐based biosensors and application in tumor theranostics
Authors: Mo Tong , Liu Xiyu , Luo Yiqun , Zhong Liping , Zhang Zhikun , Li Tong , Gan Lu , Liu Xiuli , Li Lan , Wang Huixue , Sun Xinjun , Fan Dianfa , Qian Zhangbo , Wu Pan , Chen Xiaoyuan 
Published: November 2021

AbstractAn aptamer is a short oligonucleotide chain that can specifically recognize targeting analytes. Due to its high specificity, low cost, and good biocompatibility, aptamers as the targeting elements of biosensors have been applied widely in non‐invasive tumor imaging and treatment in situ to replace traditional methods. In this review, we will summarize recent advances in using aptamer‐based biosensors in tumor diagnosis. After a brief introduction of the advantage of aptamers compared with enzyme sensors and immune sensors, the different sensing designs and mechanisms based on 3 signal transduction modes will be reviewed to cover different kinds of analytical methods, including: electrochemistry analysis, colorimetry analysis, and fluorescence analysis. Finally, the prospective advantages of aptamer‐based biosensors in tumor theranostics and post‐treatment monitoring are also evaluated in this review.
Novel amino acid‐stilbene quaternary ammonium salt fluorescent whitening agents: synthesis, optical properties, acid resistance and anti‐bacterial activity
Authors: Wan Maosheng , Luo Yanglin , Tong Zhangfa , Geng Qiaohong , Hua Li 
Published: November 2021

AbstractWith 4,4'‐diaminostilbene‐2,2'‐disulfphonic acid, 2,4,6‐trichloro‐1,3,5‐triazine and N,N‐dimethyldodecylamine as main raw materials, a stilbene quaternary ammonium salt intermediate was synthesised by condensation and quaternisation reaction under the catalysis of sodium hydroxide, and then reacted with two different amino acids to prepare novel amino acid‐stilbene quaternary ammonium salt fluorescent whitening agents. The structures of target compounds were characterized by Fourier Transform–infrared and proton nuclear magnetic resonance spectroscopy. Their photophysical properties, whitening performance and firmness in aqueous solution were assessed by means of ultraviolet absorption spectra, fluorescence emission spectra and a cotton fibre application test. The results showed that the two amino acid‐stilbene quaternary ammonium salt fluorescent whitening agents had a good whitening effect on cotton fibres and displayed strong acid resistance. Compared with the traditional fluorescent whitening agent C186, their firmness was greatly improved. The anti‐bacterial activities against three strains were evaluated in vitro. The results showed that they exhibited significant anti‐bacterial activity against the selected strains, and the respective minimum inhibitory concentration values against Candida albicans and Escherichia coli were 16.8 and 8.16 μg/ml.
Interaction of multispecies sward composition and harvesting management on herbage yield and quality from establishment phase to the subsequent crop
Authors: Komainda Martin , Muto Paul , Isselstein Johannes 
Published: November 2021

AbstractDelayed harvest during different periods of the year may help to increase the multifunctionality in terms of simultaneous flowering resource and forage provision of ley grassland. Interaction effects of harvesting regime and the grass sward composition on herbage yield and quality were therefore investigated in two multispecies swards in the present study (a simple sward with five vs. a complex sward with 16 sown species) that lasted from the establishment phase (2017) over two main production years (2018 and 2019) until the subsequent cropping phase (2020) where legacy effects were analysed by growing annual ryegrass (Lolium multiflorum gaudini). A particular drought in the year 2018 affected the botanical composition which resulted in a lucerne‐dominated complex sward and a grass‐rich simple sward thwarting the initial aims of studying complex sward treatments. Extended regrowth in early summer increased herbage yield significantly while herbage quality, weighted over the respective periods, was hardly affected by harvesting regime. No consequence of the harvesting regime resulted during the subsequent cropping phase where the plots previously grown to the lucerne‐dominated complex sward resulted in significantly greater herbage dry‐matter yield, nitrogen yield and lower weed biomass. Overall, the interaction between harvesting management and grass sward composition was never significant. It is concluded that no negative consequence for herbage quality results at extended regrowth during part of the year. However, further studies comparing complex with simple multispecies swards should consider different soil types under more humid climate.