bluuebunny/crossref_metadata_2025_split huggingface dataset:
Universal strategy towards high–energy aqueous multivalent ion batteries
Authors: Tang Xiao , Zhou Dong , Zhang Bao , Wang Shijian , Li Peng , Liu Hao , Guo Xin , Jaumaux Pauline , Gao Xiaochun , Fu Yongzhu , Wang Chengyin , Wang Chunsheng , Wang Guoxiu 
Published: January 2021

Abstract
Non–aqueous rechargeable multivalent metal (Ca, Mg, Al, etc.) batteries are promising for large–scale energy storage due to their low cost. However, their practical applications face formidable challenges owing to low electrochemical reversibility and dendrite growth of multivalent metal anodes, sluggish kinetics of multivalent ion in metal oxide cathodes, and poor electrode compatibility of flammable organic electrolytes. To overcome these intrinsic hurdles, we develop aqueous multivalent ion batteries to replace the prevailing non–aqueous multivalent metal batteries by using wide–window super–concentrated aqueous gel electrolytes, the versatile high–capacity sulfur anodes, and high–voltage metal oxide cathodes. This rationally designed aqueous battery chemistry enables the long–lasting multivalent ion batteries featured with increased high energy density, reversibility and safety. As a demonstration model, a calcium ion−sulfur||metal oxide full cell exhibited a high energy density of 110 Wh kg–1 with outstanding cycling stability. Molecular dynamics modelling and experimental investigations revealed that the side reactions could be significantly restrained through the suppressed water activity and formation of protective inorganic solid electrolyte interphase in the aqueous gel electrolyte. The unique redox chemistry has also been successfully extended to aqueous magnesium ion and aluminum ion−sulfur||metal oxide batteries. This work will boost aqueous multivalent ion batteries for low−cost large–scale energy storage.
Snail upregulates FN and LEF transcription negatively feed backed by PPAR-gamma: a general model established for Snail to transactivate mesenchymal genes
Authors: Ly Tam Minh , Chen Yen-Cheng , Lee Ming-Che , Chen Chuan Chu , Chang Hsin-Hou , You Ren-In , wu wen-sheng 
Published: January 2021

Abstract
Background Snail (SNA) is responsible for epithelial mesenchymal transition, migration and metastasis of hepatocellular carcinoma. SNA represses the transcription of the essential epithelial marker such as E-cadherin and enhances mesenchymal markers including fibronectin and lymphoid enhancer-binding factor. Our previous studies indicated that SNA, in collaboration with EGR1 and SP1, may directly activate transcription of the mesenchymal markers, matrix degradation enzyme matrix metalloproteinases (MMP9) and zinc finger E-box binding homeobox 1 (ZEB1) in HepG2 cell stimulated by the phorbol ester tumor promoter 12-O-tetradecanoyl-phorbol 13-acetate (TPA). Besides, we pinpointed a SNA binding motif (TCACA) upstream of EGR1/SP1 overlapping region on promoters. In this study, we investigated whether LEF and FN are transcriptionally regulated by SNA in a similar fashion. Moreover, a general model for SNA-upregulated mesenchymal markers is proposed.Methods RT/PCR and Western blot were used for analyzing gene expression and shRNA technology for depleting SNA. Dual luciferase assay was used for promoter activation; deletion mapping and mutagenesis were used for confirming the indicated promoter region required for transcription activation. ChIP and EMSA were used for validating the binding of the indicated transcription factor on their putative motifs. Results SNA binding motif and E/S overlapping region are required for TPA-induced transcription of LEF and FN. These were supported by TPA-induced binding of SNA and EGR-1/SP-1 on indicated promoter regions. Moreover, a peroxisome proliferator-activated receptor γ motif upstream of SNA binding motif was found to be a negatively regulatory region in TPA-induced promoter activation of FN, LEF, MMP9 and ZEB1. This was supported by that co-treatment of a PPAR-g inhibitor, GW9662, and mutation of PPAR-g binding motif enhanced TPA-induced promoter activity and expression of the aforementioned genes whereas overexpression of PPAR-g reversed it. Moreover, comprehensive screening of the SNA-upregulated mesenchymal genes revealed similar sequence architecture on the promoter regions of the candidate genes: SNA binding motif (TCACA) coupled with a downstream EGR/SP1 overlapping region and an upstream PPAR-g binding motif. Among them COX2 and COL1A1 were found to potentially exhibit the same transcription mechanisms described above. Conclusions We established a general model for SNA-upregulated mesenchymal gene expressions negatively feed backed by PPAR-g.
The Effect of&nbsp;Socio-Economic Status on Food Quality in West of Iran
Authors: Amini Mahin , najafi farid , pasdar yahya , samadi mehnoosh , karyani ali kazemi , moradinazar mahdi 
Published: January 2021

Abstract
BackgroundSocio-economic status affects many health-related outcomes and one of the most important factors is the pattern and quality of nutrition. Thus, this study is carried out to investigate the effect of socio-economic status on the quality of nutrition based on healthy Eating Index (HEI).MethodsThis is a population-based study using the baseline data from Ravansar Non-Communicable Disease cohort study. HEI calculated from Food Frequency Questionnaire. The quality of nutrition was assessed based on HEI-2015 which evaluates 13 food groups.ResultsThe mean of age of the participants was 48.02 (SD = ±8.27) years. The median and mean of HEI in the studied population were 53 and 53.69 (SD = ±7.34), respectively. Women, the educated, city dwellers, people of higher socioeconomic classes, and non-smokers had greater odds of having higher quality of nutrition. The worst score among all components of HEI were for the refined grains and then the whole grain, and the highest score was obtained for the tatal protein foods. ConclusionsDue to low nutritional quality and its adverse effects, especially in people with low socioeconomic status and villagers, the quality of nutrition, especially in the poor, should be promoted with proper interventions so as to reduce disparity in the society.
A Cross-Sectional Study of Traditional Chinese Medicine Practitioner’s Knowledge, Treatment Strategies and Integration of Practice of Chronic Pelvic Pain in Women
Authors: Arentz Susan , Smith Caroline , Redmond Rebecca , Abbott Jason , Armour Mike 
Published: January 2021

Abstract
Background Chronic pelvic pain (CPP) in women is persistent, intermittent cyclical and non-cyclical lower abdominal pain, lasting for more than 6 months. Traditional Chinese Medicine (TCM) is a popular treatment option for women’s health conditions, but little is known about how treatment for CPP is delivered by TCM practitioners. The aim of this survey was to explore practitioners understanding and treatment of women with CPP, and how they integrate their management and care into the health care system. Method An online cross-sectional survey of registered TCM practitioners in Australia and New Zealand. Survey domains included treatment characteristics (e.g. frequency), evaluation of treatment efficacy, referral networks, and sources of information that informed clinical decision making. Results One hundred and twenty-two registered TCM practitioners responded to this survey, 91.7% reported regular treatment of women with CPP. Treatment decisions were most-often guided by a combination of biomedical and TCM diagnosis (77.6%), and once per week was the most common treatment frequency (66.7%) for acupuncture. Meditation (63.7%) and dietary changes (57.8%) were other commonly used approaches to management. The effectiveness of treatment was assessed using multiple approaches, most commonly pain scales, (such as the numeric rating scale) and any change in use of analgesic medications. Limitations to TCM treatment were reported by over three quarters (83.7%) of practitioners, most commonly due to cost (56.5%) and inconvenience (40.2%) rather than safety or lack of efficacy. Integration within the wider healthcare system was common with over two thirds (67.9%) receiving referrals from health care providers. Conclusion TCM practitioners seeing women with various CPP symptoms, commonly incorporate both traditional and modern diagnostic methods to inform their treatment plan, monitor treatment progress using commonly accepted approaches and measures and often as a part of multidisciplinary healthcare for women with CPP.
Factors Influencing Malaria Morbidity and Mortality Among Under 15 Years Children in Parts of Ghana: The Role of Guardians’ Socio-Demographic Characteristics Demonstrated Through Modelling
Authors: Asare Kwame 
Published: January 2021

Abstract
Background To understand the underlying factors that led to failure to achieve the millennium development goal 6c by Ghana, we modelled guardians’ socio-demographic characteristics and the factors that could influence child health to provide directions for improving anti-malaria interventions in Ghana and sub-Sahara Africa.Methods Clinical data of 274 malaria patients, age up to 15 years, were collected from a cross-sectional study conducted in two districts in the central region of Ghana. Guardians of the children were interviewed to obtain socio-demographic characteristics and factors that could influence child health. The data obtained were analysed using multivariate GLM, type III ANOVA and multilayer perceptron network analysis.Results Of the 274 children enrolled into the study, 69.34% were between the age of 0-5 years. The median parasite density, haemoglobin and platelet were 89,923 (64,056-149,541) parasites/mm3, 8.40 (4.70-11.60) g/dL and 126 (87-146) x10^9/L respectively. 12.41% of the enrolled children had severe clinical conditions. The outcome using multivariate GLM analysis indicate a significant association between the guardians’ socio-demographic characteristics and factors that influence child health, p<0.0001. CSOC, FPOCCH, DOOSCS, and CS were all influenced by the guardians’ occupational status, p<0.0001; CSOC and MCID were influenced by the guardians’ educational levels, p<0.0001 and FPOCCH and MCID were also influenced by guardians age category, p<0.0001. The multilayer perceptron network analysis showed that the association between guardians’ socio-demographic characteristics and factors that influence child health are affected by several hidden confounding factors.Conclusion It was demonstrated an association between factors that influence child health care and guardians’ socio-demographic characteristics and this is confounded by hidden socio-economic factors and biases through modelling. I, therefore, propose a model called a network of socio-economic progenitors to provide a simple explanation for the underlying factors that influence child health in malaria-endemic areas.
Wharton Jelly Stem Cells inhibits AGS Gastric Cancer Cells through Induction of Apoptosis and Modification of MAPK and NF‑κB Signaling Pathways
Authors: Abbasi Samaneh , Bazyar Reza , Saremi Mohammad Ali , Alishiri Gholamhoseen , Seyyedsani Nasrin , Jadid Mahdieh Farhoudi Sefidan , Khorrami Afshin , Golmarz Parisa Emami , Jahangirzadeh Gholamreza , isazadeh Alireza , Hajazimian Saba , Amoodizaj Fatemeh Firouzi 
Published: January 2021

Abstract
Background and aim: Gastric cancer) GC) is one of the most common cancer with high mortality worldwide. The human Wharton's jelly stem cells (hWJSCs) can inhibit several cancer cells through several molecular pathways. Therefore, the present study aimed to investigate anticancer effects of hWJSCs conditioned medium (hWJSC-CM) and cell-free lysate (hWJSC-CL) against of GC cell line AGS and underlying signaling pathways. Methods: In this study, we evaluated the effects of hWJSC-CM and hWJSC-CL on viability, proliferation, migration, invasion, apoptosis, and MAPK and NF‑κB signaling pathways in AGS cells. Moreover, mRNA expression of genes involved in apoptosis (BAX, BCL2, SMAC, and SURVIVIN), as well as expression of proteins involved in NF-κB and MAPK signaling pathways were evaluated. Results: The obtained results showed that the hWJSC-CM and hWJSC-CL decreased viability, migration, and invasion of GC cell line AGS in a concentration and time dependent manner. We observed that the hWJSC-CM and hWJSC-CL induced apoptosis pathway through regulation of apoptosis involved genes mRNA expression. In addition, the hWJSC-CM and hWJSC-CL suppressed NF-κB signaling pathways as well as promoted MAPK signaling pathways. Conclusions: In general, our study suggested that the hWJSC-CM and hWJSC-CL inhibits proliferation and viability of GC cell line AGS through induction of apoptosis, as well as modification of NF-κB and MAPK signaling pathways.
Retrospective Cohort Study Comparing Current Third Stage Management to Expedient Squatting Third Stage Management
Authors: Cohain Judy Slome , Buxbaum Rina 
Published: January 2021

Abstract
Background: Active management of the delivery of the placenta results in 5% postpartum hemorrhage, 1% blood transfusions and an average blood loss of 500 cc. Shorter third stages are associated with decreased hemorrhage rates. The third stage can be shortened by instructing the birthing woman to squat and push out the placenta at 3 minutes postpartum. The objective of this study was to compare blood loss and PPH rates using Judy's 3,4,5 minute third stage expedient squatting protocol to variations of active and expectant third stage management among similar populations.Methodology: A retrospective cohort study was carried out comparing 1,098 planned homebirths attended in Israel, in which Judy’s 3,4,5 expedient squatting third stage technique was practiced, to 2,899 planned homebirths attended by midwives in British Columbia, Canada, where currently accepted third stage management was used. The inclusion criteria for both groups were: Singleton fetus in cephalic presentation; gestational age 37+0 to 41+6 weeks; spontaneous onset of labor; history of up to one previous cesarean; absence of significant pre-existing disease and absence of significant disease arising during pregnancy. The main outcomes were postpartum hemorrhage and manual removal of the placenta. Results: Using identical inclusion criteria and similar management, variations of active and expectant management resulted in 4% PPH over 1000 cc and 1.0% manual removal. Expedient squatting resulted in 2/1098 cases of PPH>500 cc, zero cases of PPH over 1000 cc and 0.7% manual removal. Conclusion: Judy’s 3,4,5 minute third stage management minimizes postpartum bleeding compared to other third stage protocols, reproducing postpartum hemorrhage rates indigenous to primates.
Trimester-Specific Reference Ranges for Thyroid Hormones of Pregnant Females at Tertiary Care Hospitals in Lahore, Pakistan
Authors: Mumtaz Asim , Sadiq Fauzia , Zaki Saima , Batool Hijab , Ibrahim Muhammad , Khurram Muhammad , Awan Usman Ayub , Saeed kiran , Afzal Muhammad Sohail 
Published: January 2021

Abstract
Background: The significance of investigation for diagnosing and managing thyroid dysfunction in pregnant females has been extensively documented in the medical literature. This study aimed to determine trimester-specific reference ranges for thyroid-stimulating hormones (TSH), Free T3 (FT3), and free T4 (FT4) in apparently healthy pregnant women attending tertiary care hospitals in Lahore. Methods: This cross-sectional study was conducted at two tertiary care Hospitals in Lahore, Pakistan. In this multi-centric study, initially, 500 pregnant females were enrolled from September 2019 to December 2019 who fulfilled the inclusion criteria. For measurement of serum freeT3, free T4, TSH, anti-TPO, and Thyroglobulin antibodies, 5 ml of the blood sample was drawn, under aseptic conditions, from each subject using Maglumi 800 chemiluminescence immunoassay (CLIA) system. Results: Out of 500 subjects, 23 subjects with positive anti-TPO, 19 subjects with anti-TG antibodies, and 12 subjects due to less volume of serum yielded from whole blood (serum less than 3 ml) were excluded from the analysis. Ten samples were hemolyzed and not included in the analysis. A total of 436 samples were examined for analysis. Of the remaining 436 subjects, 133 (30.5%) were from 1st Trimester, 153 (35.1%) from 2nd Trimester, and 150 (34.4%) from 3rd Trimester. As the data were non-normal, the 2.5th, 50th and 97.5th percentiles were calculated to express each group's results. Trimester specific range of TSH 0.168-4.294, 0.258-4.584 and 0.341-4.625, FT3 1.857-4.408, 1.958-4.621 and 2.025-4.821 pg/mL and FT4 8.815-18.006, 8.306-17.341 and 7.402-17.292 pg/mL. Conclusion: In this study, we established a trimester-specific reference range for our local population's thyroid function test. The results of this study have complemented the results of previous studies.
Development of novel immunotherapy based on nanoparticle co-delivering PLK1 and PD-L1 inhibitors for lung cancer treatment
Authors: Reda Moataz , Ngamcherdtrakul Worapol , Siriwon Natnaree , Bejan Daniel , Reda Sherif , Wang Ruijie , Nelson Molly , Zaidan Husam , Hoang Ngoc , Bindal Akash , Mills Gordon , Gray Joe , Yantasee Wassana 
Published: January 2021

Abstract
Immune checkpoint inhibitors (ICIs) targeting PD-L1 and PD-1 have improved survival in a subset of patients with advanced non-small cell lung cancer (NSCLC). However, only a minority of NSCLC patients respond to ICIs, highlighting the need for superior immunotherapy. Herein, we developed a nanoparticle-based immunotherapy termed ARAC (Antigen Release Agent and Checkpoint Inhibitor) to enhance the efficacy of PD-L1 inhibitor. ARAC is nanoparticle co-delivering PLK1 inhibitor, volasertib, and PD-L1 antibody. PLK1 is a key mitotic kinase that is overexpressed in various cancers including NSCLC and drives cancer growth. Inhibition of PLK1 selectively kills cancer cells and upregulates PD-L1 expression in surviving cancer cells thereby providing opportunity for ARAC targeted delivery in a positive feedback manner. ARAC reduced effective doses of volasertib and PD-L1 antibody by 5-fold in a metastatic lung tumor model and the effect was mainly mediated by CD8 + T cells. We also observed abscopal effect of ARAC in bilateral NSCLC tumor model and achieved complete cures in some mice when incorporating immune-stimulant CpG onto ARAC. Further, ARAC was well-tolerated in non-human primates. This study highlights a rationale combination strategy to augment existing therapies by utilizing our nanoparticle platform that can load multiple cargo types at once.
Development of An Implementation Process Model: A Delphi Study
Authors: Parker Gillian , Kastner Monika , Born Karen , Berta Whitney 
Published: January 2021

Abstract
Background: There is general scarcity of research on key elements of implementation processes and the factors which impact implementation success. Implementation of healthcare interventions is a complex process. Tools to support implementation can facilitate this process and improve effectiveness of the interventions and clinical outcomes. Understanding the impact of implementation support tools is a critical aspect of this process. The objective of this study was to solicit knowledge and consensus from relevant implementation science and knowledge translation healthcare experts in order to refine and validate a process model of key elements in the implementation process.Methods: A two round consensus-based modiﬁed Delphi study involving international experts in knowledge translation and implementation (researchers, scientists, professors, decision-makers) was conducted. Participants rated and commented on all aspects of the process model, including the organization, content, scope, and structure. Delphi questions rated at 75% agreement or lower were reviewed and revised. Qualitative comments supported the restructuring and refinement. A second-round survey followed the same process as Round 1. Results: Fifty-four experts participated in Round 1, and 32 experts participated in Round 2. Twelve percent (n=6) of the Round 1 questions did not reach consensus. Key themes for revision and refinement were: stakeholder engagement throughout the process, iterative nature of the implementation process; importance of context; and importance of using guiding theories or frameworks. The process model was revised and refined based on the quantitative and qualitative data and reassessed by the experts in Round 2. Consensus was achieved on all items in Round 2 and the Delphi concluded. Additional feedback was obtained regarding terminology, target users and definition of the implementation process.Conclusions: High levels of agreement were attained for all sub-domains, elements, and sub-elements of the Implementation Process Model. This validated model will be used to develop an Implementation Support Tool to be used by healthcare providers to facilitate effective implementation and improved clinical outcomes.
Photodynamic Diagnostic Ureteroscopy Using The VISERA ELITE® Video System For Diagnosis of Upper-Urinary Tract Urothelial Carcinoma: A Prospective Cohort Pilot Study
Authors: Wada Koichiro , Araki Motoo , Tanimoto Ryuta , Sadahira Takuya , Watari Shogo , Maruyama Yuki , Mitsui Yosuke , Nakajima Hirochika , Acosta Herik , Katayama Satoshi , Iwata Takehiro , Nishimura Shingo , Takamoto Atsushi , Sako Tomoko , Edamura Kohei , Kobayashi Yasuyuki , Watanabe Masami , Watanabe Toyohiko , Nasu Yasutomo 
Published: January 2021

Abstract
Background: The advantages of photodynamic diagnostic technology using 5-aminolevulinic (ALA-PDD) have been established. The aim of this prospective cohort study was to evaluate the usefulness of ALA-PDD to diagnose upper tract urothelial carcinoma (UT-UC) using the Olympus VISERA ELITE® video system.Methods: We carried out a prospective, interventional, non-randomized, non-contrast and open label cohort pilot study that involved patients who underwent ureterorenoscopy (URS) to detect UT-UC. 5-ALA HCl was orally administered before URS. The observational results and pathological diagnosis with ALA-PDD and traditional white light methods were compared, and the proportion of positive subjects and specimens were calculated.Results: A total of 20 patients were enrolled and one patient who had multiple bladder tumors did not undergo URS. Fifteen of 19 patients were pathologically diagnosed with UT-UC and of these 11 (73.3%) were ALA-PDD positive. Fourteen of 19 patients were ALA-PDD positive and of these 11 were pathologically diagnosed with UC. For the 92 biopsy specimens that were malignant or benign, the sensitivity for both traditional white light observation and ALA-PDD was the same at 62.5%, whereas the specificities were 73.1% and 67.3%, respectively. Of the 38 specimens that were randomly biopsied without any abnormality under examination by both white light and ALA-PDD, 11 specimens (28.9%) from 5 patients were diagnosed with high grade UC. In contrast, four specimens from 4 patients, which were negative in traditional white light observation but positive in ALA-PDD, were diagnosed with carcinoma in situ (CIS).Conclusions: Our results suggest that ALA-PDD using VISERA ELITE® is not sufficiently applicable for UT-UC. Nevertheless, it might be better particularly for CIS than white light and superior results would be obtained using VISERA ELITE® II video system.Trial registration: The present clinical study was approved by the Okayama University Institutional Review Board prior to study initiation (Application no.: RIN 1803-002) and was registered with the UMIN Clinical Trials Registry (UMIN-CTR), Japan (Accession no.: UMIN000031205).