bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of survival and behavior of lake whitefish following transmitter implantation using electro- or chemical immobilization
Authors: Izzo Lisa K. , Dembkowski Daniel J. , Binder Thomas R. , Hansen Scott P. , Vandergoot Christopher S. , Isermann Daniel A. 
Published: December 2024

Abstract
Background
The number of telemetry studies focused on lake whitefish (Coregonus clupeaformis) in the Laurentian Great Lakes has steadily increased over the last decade, but field tests of immobilization methods used for tag implantation, which have the potential to affect survival and behavior of fish after release, are lacking. We compared post-tagging survival and behavior of lake whitefish that were immobilized for tag implantation using electroimmobilization via a transcutaneous electrical nerve stimulation (TENS) unit or by chemical immobilization via exposure to 10% eugenol.

Results
Acoustic tags were implanted into 126 adult lake whitefish (N = 126; N = 67 TENS treatment group, N = 59 eugenol treatment group) collected from the Fox River, Wisconsin, during the spawning period in November 2021. We found no significant differences between treatments in the number of days that lake whitefish spent in the Fox River following tagging (TENS mean = 13.4 days, eugenol mean = 14.7), and also found that the proportions of fish within each treatment group that returned to the Fox River during fall 2022 (51% from TENS treatment group, 49% from eugenol treatment group) did not differ from the proportions for all fish that were confirmed to be alive at that time. The best Cormack–Jolly–Seber model indicated no differences in survival between the two treatment groups (monthly survival = 0.980, 95% CI 0.970–0.987). Fish immobilized using TENS underwent almost immediate induction and recovery from surgeries, while fish immobilized using eugenol had induction times that ranged 167–487 s (mean = 347 s) and recovery times that ranged 51–2358 s (mean = 1242 s).

Conclusions
Short- and long-term behavior (time to exit of Fox River, return to Fox River in the next spawning season) and monthly survival estimates of lake whitefish did not differ between the immobilization treatments. Either method may be suitable for immobilization during tag implantation, but the additional time needed for induction and recovery of fish when using eugenol may be a limiting factor in some field-based tagging situations.
Selective retinoid X receptor agonism promotes functional recovery and myelin repair in experimental autoimmune encephalomyelitis
Authors: Kasheke Gracious D. S. , Hendy Basmah A. M. , Dorighello Gabriel G. , Uccelli Nonthué A. , Gothié Jean-David M. , Novorolsky Robyn J. , Oulton Madison J. , Asainayagam Jude , Makarov Adam I. , Fraser Kaitlyn S. , Vuligonda Vidyasagar , Sanders Martin E. , Kennedy Timothy E. , Robertson George S. 
Published: December 2024

AbstractEvidence that myelin repair is crucial for functional recovery in multiple sclerosis (MS) led to the identification of bexarotene (BXT). This clinically promising remyelinating agent activates multiple nuclear hormone receptor subtypes implicated in myelin repair. However, BXT produces unacceptable hyperlipidemia. In contrast, IRX4204 selectively activates the retinoid X receptor (RXR). Given compelling links between RXR activation and increased myelin repair, we employed IRX4204 to investigate the impact of RXR agonism alone on functional recovery in mice subjected to experimental autoimmune encephalomyelitis (EAE). Since gait deficits are common in MS, we used machine learning to obtain highly sensitive and reliable measurements of sagittal hindleg joint movements for mice walking on a treadmill. IRX4204 not only blocked the progressive loss of knee and ankle movements but also reversed joint movement impairments in EAE mice. Our biochemical, transcriptional and histological measurements in spinal cord suggest these gait improvements reflect increased axon survival and remyelination and reduced inflammation. Using microglia, astrocytes and oligodendrocyte progenitor cells, we present additional data suggesting that IRX4204 may act on multiple glial subtypes to orchestrate myelin repair. These results inform the discovery of restorative neural therapeutics for MS by demonstrating that selective RXR agonism is sufficient for effective myelin repair. Moreover, our findings support the therapeutic potential of IRX4204 to promote functional recovery in MS.
Characteristic visual phenotypes in Korean wild mice (KWM/Hym)
Authors: Jamiyansharav Munkhdelger , Shin Haesol , Kim Boyoung , Kim Hongkyung , Han Soo Jung , Seong Je Kyung , Suh Jun Gyo , Seo Kyoung Yul 
Published: December 2024

Abstract
Background
In the last few decades, numerous efforts have been made to develop a better mouse model to overcome the current limitations of laboratory inbred mouse models such as have a weaker and simpler immune status. As part of these efforts, in Korea, the Hallym university medical genetics research team has been developing a new inbred strain of Korean wild mouse KWM/Hym. It was suggested that this strain, which is derived from wild mice, might be useful for genetic research and may become a valuable tool for overcoming some limitations seen in inbred mice that are currently used in the laboratory. Furthermore, for this study, we aimed to determine the visual phenotype of this unique strain KWM/Hym, and consider whether and if they are suitable for visual research. To analyze their visual phenotype, we performed the functional and morphological examinations in KWM/Hym mice and compared the results with laboratory mice which are the most common background strain.

Results
KWM/Hym had a thin corneal phenotype, thin but well-ordered retina due to their light body weight characteristic, and normal visual function similar to control mice. Unexpectedly, the KWM/Hym mice developed cataracts only at around 25 weeks old.

Conclusions
We suggest Korean wild mouse KWM/Hym is useful for visual experiments and could be an animal model of eye disease in humans.
The Value of LC-MS/MS in Apparent Bilateral Aldosterone Suppression in Adrenal Venous Sampling for Primary Aldosteronism
Authors: Li Wei , Zhou Qixin , He Yifan , He Wenwen , Song Ying , Hu Jinbo , Wang Yue , Luo Ting , Li Qifu , Yang Shumin 
Published: December 2024

Abstract

Context
Adrenal venous sampling (AVS) is recommended for subtyping primary aldosteronism (PA); however, it is unreliable when apparent bilateral aldosterone suppression (ABAS) occurs, defined as aldosterone/cortisol ratio in the adrenal vein being bilaterally lower than that in the inferior vena cava. The value of liquid chromatography–tandem mass spectrometry (LC-MS/MS) in PA subtyping when ABAS occurs is unclear.

Objective
This work aimed to determine whether LC-MS/MS can reduce the occurrence of ABAS and misdiagnosis of PA subtyping.

Methods
Aldosterone and cortisol in AVS samples from 219 patients with PA were measured by LC-MS/MS and immunoassay (IA). Then ABAS occurrence and misdiagnosis rate of PA subtyping were calculated.

Results
Among 219 patients with PA, 111 and 60 received nonadrenocorticotropin (non-ACTH) and ACTH-stimulated AVS, respectively, and 48 received both. In unstimulated AVS, LC-MS/MS reduced the ABAS occurrence compared to IA (10.7% [17/159] vs 3.1% [5/159]). Among 159 patients with unstimulated AVS, there were 77 patients who had final subtyping diagnosis based on postoperative outcomes. The misdiagnosis rate of IA and LC-MS/MS was 10.4% (8/77) and 9.1% (7/77), respectively. However, misdiagnosis was more common in patients with ABAS than in those without ABAS. For IA, 5 out of 6 ABAS patients were misdiagnosed, while 3/71 non-ABAS patients were misdiagnosed. For LC MS/MS, only one ABAS patient had follow-up outcome and he was misdiagnosed, while 6 of 76 non-ABAS patients were misdiagnosed. Similar results were found in ACTH-stimulated AVS.

Conclusion
LC-MS/MS measurement of aldosterone and cortisol is a new resolution when ABAS occurs.
Localized surface plasmon resonance-based optical fiber arsenic ion sensor employing Al2O3/GO nanocomposite
Authors: Banoo Fatima , Khijwania Sunil K. 
Published: December 2024

A novel optical fiber arsenic (As3+) sensor, to the best of our
knowledge, based on a
localized surface plasmon
resonance (LSPR) technique, is reported in this paper. The sensor
employs Al2O3/GO nanocomposite as the sensing film. An
experimental investigation demonstrates that the proposed sensor is
characterized with a linear response in the range of
0–20 parts per billion (ppb) As3+ concentration, high sensitivity of
0.217 nm/ppb, and remarkably low limit of detection (LOD) of
0.09 ppb, which is significantly lower than the limit set by
WHO in drinking water (10 ppb). Additionally, the sensor
exhibits a very fast response time of 0.5 s and exhibits high
selectivity towards As3+ ions over the other heavy metal ions.
Furthermore, the sensor displays a very high degree of repeatability,
stability, reversibility, and reliability. To validate the proposed
sensor’s real-world applicability, the sensor is employed to detect
arsenic in real water samples. The obtained results exhibit strong
alignment with the standard inductively coupled plasma mass
spectrometry (ICPMS) method, confirming its accuracy for real-world
arsenic detection.
Inversion method of organic matter content of different types of soils in black soil area based on hyperspectral indices
Authors: Lin Nan , Liu Yanlong , Liu Qiang , Jiang Ranzhe , Ma Xunhu 
Published: December 2024

Abstract
Soil organic matter content (SOMC) is a key factor in improving the soil fertility of arable land. Determining how to quickly and accurately grasp SOMC on a regional scale has become an important task for farmland quality monitoring. Hyperspectral imaging remote sensing technology can enable large-scale SOMC estimation, owing to its large-scale and fine spectral resolution. Enhancing the accuracy and reliability of SOM estimation models based on hyperspectral satellite remote sensing has emerged as a prominent topic of study. In this study, feature spectral indices such as difference indices (DI), ratio indices, and normalized indices were extracted using the correlation coefficient method and used as variables to construct a regression model for SOM, with a split-sample regression method employed to account for the complexity of soil types and map the corresponding spatial distribution of SOM. The results showed that the SOM estimation model, built using these feature spectral indices from hyperspectral satellite imagery, achieved high predictive accuracy, with R² values approaching 0.80 for most soil types. This demonstrates that the model effectively captures variations in SOM content across diverse soil backgrounds, highlighting its robustness and adaptability. The DI499/576 combinations, in particular, contributed significantly to prediction accuracy, demonstrating their importance as key spectral parameters for SOM estimation. Furthermore, among the three sets of feature model variables derived from the split-sample regression strategy, the enhanced vegetation indices and Soil-Adjusted Total Vegetation Index exhibited distinct contributions to different soil sample groups. This variation reveals the specific responsiveness of these indices to soil properties, which further enhances model performance in varied soil contexts. This study provides innovative methods for large-scale SOMC estimation, particularly by utilizing hyperspectral indices to enhance model accuracy across various soil types, demonstrating substantial practical significance.
Request for confirmation sequences in Czech
Authors: Oloff Florence 
Published: December 2024

Abstract
This article provides a first description of request for confirmation (RfC) sequences in spoken Czech. Based on 204 sequences from video-recorded ordinary conversations, it provides a quantitative overview of the main syntactic, lexical, prosodic and sequential features of both requests for confirmation and their responses. RfCs in Czech are typically of declarative clausal format, realized in positive polarity, and receive a confirmation. The epistemic asymmetry between the speaker of the RfC and their interlocutor seems to be mainly expressed and negotiated by a complex taxonomy of tags appended to the confirmable, response tokens, and syntactically non-minimal responses. These features represent promising topics for future, more qualitatively oriented investigations of RfCs in spoken Czech.
PENDAMPINGAN KULTUR JARINGAN BAGI PETANI BUNGA TANAMAN HIAS DI DESA CIGUGURGIRANG, PARONGPONG, BANDUNG BARAT
Authors: LIberty LIberty , Septiani Elsa Dwi , Anggraini Rahayu , Rahmadi Agung 
Published: December 2024

AbstrakTanaman hias merupakan komoditas hortikultura yang memiliki nilai ekonomi yang tinggi. Budidaya tanaman hias sudah banyak dikembangkan dengan teknologi salah satunya kultur jaringan. Kelebihan kultur jaringan dapat meningkatkan kuantitas tanaman, tidak memerlukan lahan yang luas dan Dapat dilakukan disepanjang musim. Tujuan dari pengabdian ini yaitu untuk mengedukasi dan mendampingi petani mempelajari teknik kultur jaringan skala rumah tangga. Pelatihan dilaksanakan pada pada bulan Januari-November 2023 di PT. Melano Berkah Indonesia, Desa Cigugurgirang, Kecamatan Parongpong, Kabupaten Bandung Barat. Metode pelatihan yaitu memberi edukasi dan praktik kultur jaringan skala rumah tangga. Kegiatan pelatihan meliputi materi kultur jaringan, karantina tanaman, sterilisasi ruangan, sterilisasi alat, pembuatan media, sterilisasi eksplan, inisiasi, subkultur, dan aklimatisasi. Hasil pelatihan ini petani antusias belajar dan sudah bisa melakukan penanaman teknik kultur jaringan dengan baik. AbstractOrnamental plants are horticultural commodities that have high economic value. Ornamental plant cultivation has been developed using technology, one of which is tissue culture. The advantages of tissue culture can increase the quantity of plants, does not require a large area of land and makes shipping easier because the plant seeds produced are sterile. The aim of this service is to educate and assist farmers in learning tissue culture techniques on a household scale. Training will be held in January-November 2023 at PT. Melano Berkah Indonesia, Cigugurgirang Village, Parongpong District, West Bandung Regency. The training method is to provide education and practice of tissue culture on a household scale. Training activities include tissue culture material, plant quarantine, room sterilization, equipment sterilization, media making, explant sterilization, initiation, subculture and acclimatization. As a result of this training, farmers were enthusiastic about learning and were able to plant tissue culture techniques well.
A review of computer vision applications in litter and cleanliness monitoring
Authors: Kumar Ashwani , Sudarshan Lakka Bovina Naga , Kumar Amit , Kumar Rajesh 
Published: December 2024

Municipal solid waste management struggles with manual processes, affecting data accuracy and street cleanliness monitoring. Recent research highlights computer vision as a solution for automated litter detection, improving efficiency and reducing costs. This study reviews 65 studies on computer vision in urban waste management, using PRISMA 2020, to address litter and cleanliness in urban areas. The study is divided into three parts: (a) dataset curation, (b) model training, and (c) comparative analysis and challenges. There are five steps in dataset curation: (a) set the objective, (b) acquisition, (c) pre-processing, (d) annotation, and (e) splitting. The datasets utilised in these studies range from 114 to 110 988 images, encompassing diverse environmental conditions to support the training of machine learning models. Furthermore, the choice of machine learning algorithms employed in these studies is diverse, from traditional methods such as Random Forest to advanced deep learning techniques such as convolutional neural network (CNN), R-CNN (region-based CNN), and the recent YOLO (You Only Look Once) model. The studies underscore the extensive application of the F-score metric, alongside other metrics such as accuracy, average precision, error rate, and mean average precision, with F-score values reported to reach as high as 0.93.
A prospective study to compare the outcome of patellar fractures treated with tension band wiring versus cannulated cancellous screws fixation
Authors: Yadav Vinod Ramlal , Jyothsna Rachuru Venkata , Ravindran Biju 
Published: December 2024

The largest sesamoid bone in the body, the patella, is essential to the stability and functionality of the knee. It functions as a fulcrum to adhere to the patellar ligament and quadriceps tendon, increasing the quadriceps' effectiveness. Both static elements (ligaments and the joint capsule) and dynamic elements (muscles such as the hamstrings and quadriceps) are necessary for patella-femoral stability. Both direct trauma and indirect causes, such as falling when your quadriceps are clenched, can result in fractures. While direct blows might result in comminution and soft tissue injury, indirect injuries usually cause damage to the tendons or bones.There are different ways to treat fractures. For fractures with less than 2 mm displacement, non-operative treatments can be used. Surgical alternatives include screw fixation, tension band wiring and partial or complete patellectomy. It is a comparative randomized control trial.The purpose of this study is to evaluate the cannulated cancellous screws techniques in terms of safety and outcome when compare to tension band wiring for treatment of displaced transverse patellar fractures. A longitudinal observational study was carried out in the department of orthopaedics for a time period of 20 months from November 2020 to June 2022. Patients were divided into two groups one treated with cannulated cancellous screws and another treated with tension band wiring then followed up at intervals of 1st, 3rd and 6 month Results were evaluated with regard to union rate, ROM, pain score, functional outcome, complications and hardware removal rates. The mean radiological union was 9.2 weeks in cannulated cancellous screws group and 9.73 weeks in tension band wiring group, The mean knee ROM was 135° in cannulated cancellous screws Group and 129° in tension band wiring group andfunctional score in cannulated cancellous screws group was 66.7% excellent, 26.6% good and 6.7% poor outcomes. In tension band wiring group functional score was 46.6% excellent, 40% good and 13.4% poor outcomes as per WEST’S Criteria. Complications encountered in tension band wiring group were more with respect to hardware prominence, soft tissue irritation and hardware removal rates than cannulated cancellous screws group.Cannulated cancellous screws for treatment of displaced transverse patella fracture are safe and biomechanically stable implant with lesser degree of soft tissue irritation when compared to tension band wiring.
Sexuality amongst adolescents in present era: An observational cross sectional study
Authors: Verma Divya , Agarwal Anand Kumar , Singh Srishti , Singh Saurabh Kumar , Sharma Keshav 
Published: December 2024

Every individual goes through the storm and stress of intense physical and mental changes during adolescence. Awareness and knowledge about sexuality and sex during this crucial phase decides the pattern and style of their life. Gen Z has been raised in era of internet revolution. In the absence of nationwide comprehensive sexual education curriculum in schools, digitalization opens up numerous platforms of learning regarding the sexuality. To determine the knowledge among rural and urban adolescents regarding sexuality, safe sex and their source of information. An observational cross-sectional study was conducted in 2 co-educational rural and urban schools each of Sitapur district on 553 students aged 12-18 years of either sex. A self-report questionnaire was administered to test their knowledge and perception regarding sex and sexuality and source of information. Data was analysed using Epi Info7. Categorical variables were expressed as number and percentage.In present study rural adolescent has lesser number of opposite sex friends than urban counterpart. They have more physical groups while urban adolescents have more online groups. The preferred mode of interaction among urban adolescents is digital media. Lesser number of rural adolescents have knowledge about methods of safe sex, route of transmission and prevention of HIV. Major source of information was digital platform among urban adolescents.There is knowledge gap among adolescents from rural area in present study due to social barriers and less access to internet. Thus, in this present era emphasis should be on proper sex education through different digital platforms which can play a pivotal role in delivering the facts about sexuality and appropriate grooming of adolescents.
Evaluation of febrile neutropenia in diagnosed cases of acute lymphoblastic leukemia in children
Authors: Gupta Nikhil , Sharma Novneet , Jamwal Mona , Digra Sanjeev K , Parihar Ravi Kumar 
Published: December 2024

Febrile neutropenia is a serious and most common complication of cancer therapy. A single oral temperature of greater than or equal to 101 F, or a temperature of greater than or equal to 100.4 F for at least an hour with ANC &#60;500/µL, or less than 1000/µL with a predicted fall to less than 500/µL in the following 48 hours, are the criteria for its clarification. is to study clinical profile of febrile neutropenia in ALL patients and to study the susceptibility patterns of pathogens and bacterial spectrum in culture positive febrile neutropenic patients in ALL. It was a retrospective study. Patients undergoing treatment and meeting the criteria for Febrile Neutropenia were included in study. The study was a retrospective that lasted a year. Children with febrile neutropenia undergoing chemotherapy were included in the research. The study emphasize the common infectious agents, their antibiotic sensitivity pattern. ANC being the most important parameter it was found that 15 patients (37.5%) had ANC &#60;500/ microL and 25 patients (62.5%) had ANC between 500-1000 /microL which had fallen to &#60;500/microL in next 24 hours. : Long-term neutropenia was linked to an increased risk of FN, and infections with a preponderance of Gram-negative bacteria were found in about 32.5% of patients.