bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin-17 is Involved in Neuropathic Pain and Spinal Synapse Plasticity on Mice
Authors: Sun Jia-Lu , Dai Wen-Jing , Shen Xin-Yuan , Zhang Yu-Qiu , Lü Ning 
Published: June 2021

Abstract
Background: Neuropathic pain seriously affects people’s life, but its mechanism is not clear. Interleukin-17 (IL-17) is a proinflammation cytokine and involved in pain regulation. Our previous study found that IL-17 markedly enhanced the excitatory activity of spinal dorsal neurons in mice spinal slices. The present study attempts to explore if IL-17 contributes to neuropathic pain and spinal synapse plasticity.Methods：A model of spared nerve injury (SNI) was established in C57BL/6J mice and IL-17a mutant mice. The pain-like behaviors was tested, and the expression of IL-17 and its receptor, IL-17RA, was detected. C-fiber evoked field potentials were recorded in vivo. Results: In the spinal dorsal horn, IL-17 predominantly expressed in the superficial spinal astrocytes and IL-17RA expressed mostly in neurons and slightly in astrocytes. The SNI-induced static and dynamic allodynia was significantly prevented by pretreatment of neutralizing IL-17 antibody (intrathecal injection, 2 μg/10 μL) and attenuated in IL-17a mutant mice. Post-treatment of IL-17 neutralizing antibody also partially relieved the established mechanical allodynia. Moreover, spinal long-term potentiation (LTP) of C-fiber evoked field potentials, a substrate for central sensitization, was suppressed by IL-17 neutralizing antibody. Intrathecal injection of IL-17 recombinant protein (0.2 μg/10 μL) mimicked the mechanical allodynia and facilitated the spinal LTP. Conclusions: These data implied that IL-17 in the spinal cord played a crucial role in neuropathic pain and central sensitization.
Pregnancy outcome in late-onset severe OHSS patients following different ascetic fluid drainage and comparison with the non-OHSS IVF patients
Authors: YUN Ren , li rong , Shuo Yang , Rui Yang , hong Song Dong , Jie Qiao 
Published: June 2021

Abstract
Introduction

Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic serious complication of controlled ovarian stimulation during assisted reproductive technology, the aim of our research is to explore the differences of pregnancy outcome between severe OHSS patients and the non-OHSS IVF patients, and also compare the pregnancy ourtome of different ascites drainage way.

Design

This is a retrospective cohort analysis carried out in a University-affiliated reproductive center from 2012 to 2019, between 359 women with severe OHSS following ascites draining and 345 non-OHSS women (matched by age and retrieved oocyte number).We examined the rates of clinical pregnancy, multiple pregnancies, miscarriage, live birth and preterm delivery between the two groups. Within the OHSS group (central venous catheter group vs paracentesis group), odds ratios (ORs) and 95% confidence intervals (CIs) of measure of clinical pregnancy were also analyzed.

Result

The biochemical pregnancy loss rate of non-OHSS patients was significantly higher than that of severe OHSS patients (11.1% vs 0.6%), the live birth, multiple pregnancy (twin pregnancy) was much more in severe OHSS group than in control group(88.9% vs 73.5%; 57.6% vs 28.1% respectively), and there was no significant difference between singleton and twin preterm rate and neonatal birth weight between OHSS and control group. Binary logistic regression analysis revealed multiple pregnancy and long-protocol showed relatively high odds ratio, suggest that the

long-protocol of COS and multiple pregnancy are the risk factor of severe OHSS.

Among severe OHSS following ascites draining, in comparison with abdominal paracentesis and central venous catheter, the ascites volume in central venous catheter group was much more than abdominal paracentesis group, there was significant difference between the two groups. There was no significant difference between the two groups in comparison of live birth, and the birth weight of singleton and twin between these two groups. but the premature birth rate of singleton in group 1 was 9.3% (10/107), whereas in group 2, it was 3.75%.

Conclusion

In conclusion, this preliminary report suggests that for the IVF-ET patients, less basic follicle number, GnRH antagonist cycle, singleton maybe the protective factors for the late-onset OHSS. Paracentesis and central venous catheter are all the effective modality to manage the ascites for the severe OHSS patients. For the patients who have the possibility of repeated paracentesis, catheter is a safe and effective way.
Production Optimization and In Vitro Evaluation of Anti-Proliferative, Anti-Oxidant and Anti-Inflammatory Potential of the Antibacterial Peptide MAFP9
Authors: Raghavan Rekha Mol Kollakalnaduvil , Pannippara Manzur Ali , Kesav Sapna , mathew Abraham , Bhat Sarita G , A Mohamed Hatha A , K Elyas K 
Published: June 2021

Abstract
Peptides are important natural bioactive agents obtained from various sources including microbes, plants and animals. The present study involved the optimization and physicochemical characterization of an antibacterial peptide MFAP9 produced by a marine fungus Aspergillus fumigatus BTMF9 and its vitro evaluation of antiproliferative, antioxidant and anti-inflammatory potential. Antiproliferative activity was performed using A549 lung carcinoma and normal L929 fibroblast cell lines employing MTT assay method. Antioxidant activity was assessed using DPPH assay and Cox2 inhibition assay of peptide treated LPS stimulated THP1 cell lines was used for anti-inflammatory studies. The results of in vitro antiproliferative activity assessment showed that MFAP9 was cytotoxic with an IC50 value of 29 µg/mL towards A549 cells. Fluorescence microscopic analysis revealed apoptotic features such as chromatin condensation and alterations in the size and the shape of cells after treatment with MFAP9 at a concentration 50 µg/mL. The DPPH radical scavenging activity of MFAP9 exhibits reasonably higher scavenging activity compared to that of standard antioxidant at same concentration level. Moreover, MFAP9 showed significant anti-inflammatory activities. Time course experiment was conducted with the Czapek- Dox medium and optimized the rate of MFAP9 production. The study on various physico-chemical parameters on the stability revealed that the maximum active temperature of MAFP9 is 50oC, stable in basic pH and 1 mM concentration of Ni2+, Cd2+ and Mg2+ promotes the peptide activity. Hopefully, by employing the advancements in proteomics, bioinformatics, and modification strategies, MFAP9 could emerge as novel promising therapeutic drug in future for various clinical applications.
Assessment of Naturally Occurring Radioactive Materials (NORM) in Mining Residues and Environmental Material from Sefwi, Awaso Bauxite Mine
Authors: Moipone Matjele , Adukpo Oscar K. , Tandoh Joseph B. 
Published: June 2021

Abstract
Gamma ray spectrometry was used to quantify level of NORM in mining residues sampled at Awaso bauxite mine and surrounding communities. The radionuclides of interest were 238U, 232Th and 40K and the radioactivity levels were determined in soil, bauxite ore, red mud and water samples from wells. The radioactivity concentrations in soil, bauxite ore and red mud 238U, 232Th and 40K were 18.01±1.96 Bqkg-1, 19.07±2.12 Bqkg-1 and 103.21±1.74 Bqkg-1; 39.42±4.18 Bqkg-1, 97.32±10.63 Bqkg-1 and 14.68±1.82 Bqkg-1; 44.85±4.79, 64.23±6.58 and 125.30±18.72 Bqkg-1. The activity levels for both 232U and 232Th were above world-wide average values while Potassium-40 levels were lower. The mean activity concentration values of 238U, 232Th and 40K in water samples were 1.49±0.45 Bql-1, 3.68±0.69 Bql-1 and 15.69±0.28 Bql-1 respectively and were within the world average activity concentrations except for bauxite ore and red mud. The committed effective dose was 0.74 mSv and annual effective dose estimated to be 0.136 mSv which is below recommended dose limit of 1 mSvyear-1 for public exposure.
Policy, Service, and Training Provision for Women Following a Traumatic Birth: An International Mapping Study
Authors: Thomson Gill , Diop Magali Quillet , Stuijfzand Suzannah , Horsch Antje 
Published: June 2021

Abstract
Background: High numbers of women experience a traumatic birth, which can lead to Post Traumatic Stress Disorder (PTSD) onset, and negative and pervasive impacts for women, infants and families. Policies, suitable service provision, and training are needed to identify and treat psychological morbidity following a traumatic birth experience, but currently there is little insight into whether and what is provided in different contexts. The aim of this study was to map policy, service and training provision for women following a traumatic birth experience in different European countries. Methods: A survey was distributed as part of the COST Action “Perinatal mental health and birth-related trauma: Maximizing best practice and optimal outcomes”. Questions were designed to capture ‘country level data’; ‘care provision’ (i.e., national policies or guidelines for the screening, treatment and/or prevention of a traumatic birth, service provision), and nationally mandated pre-registration and post-registration ‘training’ for maternity professionals. Results: Eighteen countries participated. Only one country had national policies regarding the screening, treatment and prevention of a traumatic birth experience/PTSD. Service provision was provided formally in six countries (33%), or informally in the majority (78%). In almost all countries (89%), women could be referred to specialist perinatal or mental health services. Services tended to be provided by midwives, although some multidisciplinary practice was apparent. Thirty-nine percent of the countries offered ‘a few hours’ professional/pre-registration training but none offered nationally mandated post-registration training. Conclusions: A traumatic birth experience is a key public health concern. Evidence highlights important gaps regarding formalized care provision and training for care providers.
Perceptions, Facilitators And Barriers of Physical Activity Among People Living With HIV: A Qualitative Study
Authors: Kitilya Brenda , Sanga Erica , PrayGod George , Peck Robert , Kavishe Bazil B , Ditlevsen Kia , Olsen Mette F 
Published: June 2021

Abstract
BackgroundPeople Living with HIV (PLWH) have low levels of physical activity. Understanding of perceptions, facilitators and barriers of physical activity in this population is of importance for future perspectives in developing contextual based intervention to improve physical activity in PLWH. MethodThis study explored perceptions, facilitators and barriers of physical activity in PLWH using sixteen in-depth interviews and three focus group discussions conducted in Mwanza, Tanzania. The interviews and discussions were audio recorded, transcribed and translated into English. Transcripts were coded and analysed deductively using thematic content analysis. The themes were interpreted and presented using the social ecological model. ResultsForty-three PLWH age 23-61 years old participated in this study. The findings showed that, PLWH perceived physical activity was beneficial to their health and that their perceptions and practices were rooted within the existing gender stereotypes and roles in the community. In addition to that, house-chores and income generating activities were perceived as sufficient physical activity to achieve adequate levels of physical activity. As facilitators, social support and interactions from family and friends were appraised positively as motivating factors of physical activity in this population. Indeed, at individual to community/environmental levels, PLWH reported barriers of physical activity were lack of time, money, and availability of facilities, as well as lack of social groups participating in physical activity in the communities and inadequate information on physical activity from health care providers at ART clinics. HIV disease was not reported as a main barrier of physical activity but rather stipulated from family members as a reason not to support physical activity in PLWH. ConclusionThe findings demonstrated differing perceptions, facilitators and barriers of physical activity among PLWH. Interventions addressing physical activity awareness, gender stereotypes and roles relating to physical activity performances from individual to community level, building supportive environment and infrastructures are needed to elevate physical activity levels in PLWH particularly in low and -middle income countries.
The Effect of Alcoholic Extract of Thymus Vulgaris on Hepatic Enzymes Activity and Apoptosis-Related Gene Expression in Streptozotocin-Induced Diabetic Rats
Authors: Azimi Mohammadreza , Ahmadi Elnaz , Aghaie Fatemeh , Orafei Mohammad , Ahmadi Armita , Ranjbary Ali Ghorbani 
Published: June 2021

Abstract
Many diseases, including diabetes, are involve in the development of liver disorders through changes in the expression of genes such as apoptosis-related gene. In the present study, the effect of alcoholic extract of Thymus Vulgaris on Hepatic Enzymes Activity and apoptosis-related gene expression in streptozotocin-induced diabetic rats. In this study, 50 adult male Wistar rats weighing approximately 200–220 g were divided into five groups. Diabetes was induced by intraperitoneal injection of STZ (60 mg/kg). Following 18 days, all the animals in different groups were weighed and blood samples were taken from their cardiac veins. GC analysis revealed 45 different compounds in the Thymus Vulgaris, including; thymol (39.1%), p-cymene (20.63%), γ-Terpinene (14.85%). The results showed a significant increase in liver enzymes (AST, ALT and ALP) in diabetic or streptozoic mice compared to the control group (healthy mice) (p < 0.0001). The level of liver enzymes (AST, ALT and ALP) in rats treated with doses 200 mg/kg and 400 mg/kg of thymus vulgaris extract showed a significant decrease in these enzymes in comparison with diabetic rats (p < 0.0001). The expression of caspase 3 and 9 genes in the groups treated with thyme significantly decreased compared to diabetic mice (P < 0.0001) and the expression of Bcl in the group receiving 400 mg / kg of thyme significantly increased compared to diabetic mice (P = 0.0001). Due to its antioxidant compounds, thyme improves the liver tissue cells in streptozotocin-induced diabetic mice by reducing caspases 3 and 9 as well as increasing Bcl-2.
Evaluating the Profile Geochemical Characteristics and Environmental Risk Prediction of Typical Sulfide Tailing Ponds Through Multiple Methods
Authors: Zhang Liqun , Chen Xing , Jiang Chunlu , Zheng Liugen , Xia Yiming , Qiu Zheng 
Published: June 2021

Abstract
Abandoned tailings generated from copper mining are exposed to the environment for a long time will cause related risks, such as stability, landslides, surface and groundwater pollution, acid mine drainage (AMD) and secondary mineral deposits. This research started from multiple methods and comprehensively assessed the current status of mine tailings through the joint application of geophysics, geochemistry and mineralogy techniques to identify relevant environmental hazards. A thick oxidized hardened layer was formed on the surface of the tailings dam, but there were still faults or crack that affected its structural stability. According to the low-resistivity distribution of the tailings, the surface oxide compaction was judged, and the existence of high-resistivity cracks judged the potential migration path of heavy metals (HMs). The microscopic morphology and existing mineral phases of tailings particles at different depths in the profile were determined by SEM and XRD of representative samples as the main feature of iron crystalline phase. As the profile depth increased, the minerals such as calcite, pyrite and goethite gradually appeared. In addition, Cr and As were no risk, Cu, Zn, Pb were low risk, Ni was medium risk, and Cd was high risk in risk assessment code (RAC) analysis of the tailings. Judging whether HMs in the tailings are hazardous substances according to the results of toxicity characteristic leaching procedure (TCLP), it was found that the leaching contents of Cu, Zn, Pb, Cr, Ni, As were all lower than the limit, while the leaching content of Cd was higher than the limit, and additional attention should be paid to Cd pollution.
Integrated Sustainable Childhood Pneumonia and Infectious Disease Reduction in Nigeria (INSPIRING) Through Whole System Strengthening in Jigawa, Nigeria: Study Protocol For a Cluster Randomised Controlled Trial
Authors: King Carina , Burgess Rochelle , Bakare Ayobami , Shittu Funmilayo , Salako Julius , Bakare Damola , Uchendu Obioma C , Iuliano Agnese , Isah Adamu , Adams Osebi , Seriki Ibrahim , Magama Abdullahi , Ahmed Tahlil , Ahmar Samy , Cassar Christine , Valentine Paula , Olowookere Temitayo Folorunso , MacCalla Matthew , Graham Hamish R , McCollum Eric D , Falade Adegoke G , Colbourn Tim 
Published: June 2021

Abstract
BackgroundChild mortality remains unacceptably high, with Northern Nigeria reporting some of the highest rates globally (e.g. 192/1000 live births in Jigawa State). Coverage of key protect and prevent interventions, such as vaccination and clean cooking fuel use, are low. Additionally, knowledge, care-seeking and health system factors are poor. Therefore, a whole systems approach is needed for sustainable reductions in child mortality.MethodsThis is a cluster randomised controlled trial, with integrated process and economic evaluations, conducted from January 2021 – September 2022. The trial will be conducted in Kiyawa Local Government Area, Jigawa State, Nigeria, with an estimated population of 230,000. Clusters are defined as primary government health facility catchment areas (n=33). The 33 clusters will be randomly allocated (1:1) in a public ceremony, and 32 clusters included in the impact evaluation. The trial will evaluate a locally adapted ‘whole systems strengthening’ package of three evidence-based methods: community men’s and women’s groups; Partnership Defined Quality Scorecard; healthcare worker training, mentorship and provision of basic essential equipment and commodities. The primary outcome is mortality of children aged 7 days to 59 months. Mortality will be recorded prospectively using a cohort design, and secondary outcomes measured through baseline and endline cross-sectional surveys. Assuming the following, we will have a minimum detectable effect size of 30%: a) baseline mortality of 100 per 1000 livebirths; b) 4,480 compounds with 3 eligible children per compound; c) 80% power; d) 5% significance; e) intra-cluster correlation of 0.007; f) coefficient of variance of cluster size of 0.74. Analysis will be by intention-to-treat, comparing intervention and control clusters, adjusting for compound and trial clustering. DiscussionThis study will provide robust evidence of the effectiveness and cost-effectiveness of community-based participatory learning and action, with integrated health system strengthening and accountability mechanisms, to reduce child mortality. The ethnographic process evaluation will allow for a rich understanding of how the intervention works in this context. However, we encountered a key challenge in calculating the sample size, given the lack of timely and reliable mortality data, and the uncertain impacts of the COVID-19 pandemic. Trial registrationISRCTN 39213655, registered 11th December 2019
Switching to Ziv-aflibercept In Resistant Diabetic Macular Edema Non Responsive to Ranibizumab Injection
Authors: Nawar Amin E. , Wasfy Tamer , Shafik Heba M. 
Published: June 2021

Abstract
Background: Diabetic macular edema (DME) is a leading cause of visual loss in diabetic patients and is managed using multiple anti-vascular endothelial growth factor (VEGF) agents such as bevacizumab, ranibizumab and aflibercept. The present study evaluates effectiveness of intravitreal injection of ziv-aflibercept in resistant diabetic macular edema.Methods: This is a prospective interventional study that was carried out on 59 eyes of 40 diabetic patients with diabetic macular edema resistant to three prior consecutive ranibizumab injections. On all patients, thorough ophthalmic evaluation including optical coherence tomography was performed. In patients with persistent intraretinal or subretinal fluid, ziv- aflibercept 1.25 mg (0.05 ml) was administered by intravitreal injection monthly during the 6month study period from June to December 2019.Results: The central macular thickness (CMT) decreased significantly from 395.08±129.9 um at baseline to 282.39±95.278, 245.36±79.861 and 201.17±54.042 after one, three and six months of treatment respectively (p < 0.001). Best corrected visual acuity (BCVA) in log MAR units was significantly improved from 0.95±0.21 to 0.51±0.23 after six months (p = 0.001). After treatment, negative correlations were detected between age, number of injections, duration of DM and level of glycated hemoglobin (HbA1C) and variation of both CMT and BCVA. The only significant predictor for low final CMT after six months of injection was the CMT after three months of injection (p = 0.001).Conclusion: Ziv-aflibercept is a highly effective and safe drug in cases of DME resistant to previous ranibizumab injections especially in low-income countries.This study was retrospectively registered at clinicaltrials.gov (ID: NCT04290195) on 26-2-2020
Pre-Pandemic SARS-CoV-2 Potential Natural Immunity Among Population of Central Africa
Authors: SOURIS Marc , TSHILOLO Léon , PARZY Daniel , INGOBA Line LOBALOBA , NTOUMI Francine , KAMGAING Rachel , MBONGI Destin , PHOBA Baltazar , TSHILOLO Marie-Anasthasie , MBUNGU René , SOSSO Martin Samuel , FAINGUEM Nadine , MORAND Pierre , GONZALEZ Jean-Paul 
Published: June 2021

Abstract
More than a year after the emergence of COVID-19, significant regional differences in terms of morbidity persist, showing lower incidence rates in central Africa. The work reported here aims to test for a pre-pandemic natural immunity among populations in this region. To identify such pre-existing immunity, sera samples collected before the emergence of COVID-19 were tested to detect IgG antibodies reacting against SARS-CoV-2 proteins of major significance. Sera samples from blood donors of France were used as control. The results showed a statistically significant difference for antibodies prevalence between the samples collected in central Africa and the control samples. Our results suggest that in the central African sub-region the populations have been potentially pre-exposed before the COVID-19 pandemic to the antigens of a SARS-CoV-2-like virus.
Synthesis and Acivity of Chiral Amino Alcohols and Amides as New Cholinesterase Inhibitors for Alzheimer's Disease
Authors: KOCAKAYA Safak OZHAN , Yener Ismail , Ertaş Abdulselam , Karakaplan Mehmet 
Published: June 2021

Abstract
A series of biological active compounds 1–14 have been synthesized and used as potential inhibitors for AChE and BuChE. Potential inhibitor efficacy of these molecules to the target enzymes have been searched in vitro and theoretical by dock and molecular dynamic calculations. The results show that chiral amino alcohol compounds 6, 7 and 9 exhibited good value for medication scores. Among the tested compounds the best inhibition activities have been obtained with compounds 6 for AChE and BuChE by 87.68 and 92.46 % values, respectively at 50µg/mL concentration. The anticipated value of 6 also justified superb correlation with invitro statistics and it could be taken into consideration as drug candidate molecule for designing of novel drug. Potential inhibitory outcomes of those molecules on the right track proteins were investigated the use of Docking and Molecular Dynamics calculations. Dock score evaluation and Lipinski parameters have been proven those ligands are ability inhibitors against applicable enzymes. Our findings endorse that related compounds can be applied as a capacity supply of anti-alzheimer active molecules for designing novel products.