bluuebunny/crossref_metadata_2025_split huggingface dataset:
EVALUASI PELAKSANAAN SISTEM SURVEILANS HEALTHCARE ACQUIRED INFECTIONS (HAIS) DI RSU HAJI SURABAYA TAHUN 2020
Authors: Rosyida Aulia , Yamani Laura Navika , Mudjianto Dwiono 
Published: October 2021

Healthcare Acquired Infections (HAIs) or nosocomial infections are infections that occur in patients during hospitalization. HAIs are classified into 4 namely Catheter-Associated Urinary Tract Infection, Surgical Site Infection, Ventilator-Associated Pneumonia, and Blood Stream Infection. The purpose of this study is to evaluate the implementation of the HAIs surveillance sistem. The research method used is a descriptive evaluation study with an observational research design. The data sources used are the Reports and dissemination of HAIs Surveillance at RSU Haji Surabaya in 2019-2020. The results showed that HAIs cases at RSU Haji Surabaya in 2020 were 0.03%. The problems in the implementation of HAIs surveillance are the accuracy and completeness of data reports, implementation support facilities, and employee participation in training has not been running optimally, application bundle items are under repair, and job rotation for employees. Conclusion : the implementation of HAIs surveillance activities is in accordance the Regulation of the Peraturan Menteri Kesehatan Republik Indonesia Number 27 of 2017 Tentang Pedoman Pencegahan Dan Pengendalian Infeksi di Fasilitas Pelayanan Kesehatan, but the implementation still running unoptimally, researchers recommend the implementation of reward and punishment activities for surveillance officers as a solution to improve the sistem surveillance.
EVALUATION OF THE IMPLEMENTATION OF ACTIVE FIRE PROTECTION SYSTEM AT ERNALDI BAHAR SUMATERA SELATAN
Authors: Nurhaliza Titi , Andarini Desheila 
Published: October 2021

Introduction: The hospital is a place that is quite prone to fire hazards. Ernaldi Bahar has various characteristics of activities that have the potential to cause fires such as medical and administrative activities that use electrical installations as well as the use of intensive fire sources in kitchens, laundry or generator rooms as well as the presence of relatively high burning materials sourced from various medicines, chemicals, LPG gas cylinders and oxygen. The purpose of the research is to evaluate the implementation of active fire protection system at Ernaldi Bahar. Methods: This study uses qualitative methods with observational approaches, interviews and document studies. Result: The results showed that the active fire protection system at Ernaldi Bahar Hospital is well categorized with a percentage of 69% value with the results of fire alarm analysis based on Kepmen PU No.10 Year 2000, NFPA 72 and SNI 03-3985-2000 (81%), fire detector analysis based on NFPA 2000 and SNI 03-3985-2000 (100%), apar analysis based on NFPA 10 (89%), building hydrant analysis based on Permen PU No.26 Year 2008 and SNI 03-1745-2000 (73%), analysis of page hydrants based on Permen PU No.26 Year 2008 and SNI 03-1745-2000 (73%), and Sprinkler analysis based on SNI 03-3989-2000 (0%). Conclusion: Active fire protection system has been implemented well with a percentage of 69% value but it need some improvement in maintenance, placement, recording and completeness of active fire protection system components. Keywords: Hospital, Fire, Active Fire Protection System
Analysis Of The Application Of Occupational Safety and Health (K3) Management To Radiation At The Radiology Installation Of Ernaldi Bahar Hospital, South Sumatra Province
Authors: Wahyuni Rizki Dien , Andarini Desheila 
Published: October 2021

Introduction: The radiation dose is not controlled then the dose value will be accumulated. Then the dose received will be higher so that it can cause a drastic reduction in the number of lymphocytes. The purpose of this study was to analyze the application of occupational safety and health (K3) management to radiation at the radiology installation of Ernaldi Bahar Hospital, South Sumatra Province. Methods: This study uses qualitative methods with observational approaches, interviews and document studies. Result: The application of radiation safety management requirements at the radiology installation of Ernaldi Bahar Hospital, South Sumatra Province has not been carried out optimally. The application of radiation protection requirements, application of technical requirements, application of safety verification and health monitoring of radiation workers have been carried out properly. Conclusion: The implementation of occupational health and safety management on radiation at the radiology installation of Ernaldi Bahar Hospital, South Sumatra Province, has been going well. Keywords: Radiation, Management to Radiation, X-ray
HUBUNGAN KUALITAS TIDUR DENGAN 5 INDIKATOR SINDROMA METABOLIK PADA PERAWAT DI RUMAH SAKIT UMUM PUSAT HAJI ADAM MALIK MEDAN
Authors: Simon Simon 
Published: October 2021

ABSTRACTHigh death rate caused by non-transmitted diseases in the world is begun with metabolic syndrome in human beings such the increase in IMT (Body Mass Index) to be obesity, the increase in blood pressure to be hypertension, the increase in blood glucose to be diabetes mellitus, and abnormality of triglycerides, and HDL cholesterol. Many factors which trigger the indicator abnormality, and of them is bad sleep quality. The research used cross sectional design by analyzing the correlation between sleep quality and 5 metabolic syndrome indicator in female nurses in the operation room of Adam Malik Medan hospital. Sleep quality was measured by using questionnaire of Pittsburg Sleep Quality Index (PSQI), body height and weight were measured to get IMT value, blood pressure was measured by using tensimeter, blood glucose and blood lipid were measured by getting the respondent’s vena blood samples. The data were processed and analysis with independent t-test.The result with independent t-test showed that there was significant difference in IMT (sig=0,003), systolic blood pressure (sig=0,028), and fasting blood glucose content (sig=0,00). However, there was no significant difference in trigliyceride content (sig=0,519), HDL cholesterol content (sig=0,300),). The conclusion was that sleep quality was correlated with three metabolic syndrome indicators: IMT, blood pressure, and blood glucose content, but there was no correlation with trigliyceride and HDL cholesterol.Keywords: Sleep Quality, Metabolic Syndrome , T-Test,
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management
Authors: Qing Gao , Zhiyuan Wu , Jinge Yu , Yuqing Miao , Zuoguan Chen , Yongpeng Diao , Jinfeng Yin , Junnan Jia , Yijia Guo , Weimin Li , Yongjun Li 
Published: October 2021

Objectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients’ health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral blood mononuclear cells (PBMCs) in these patients and to explore potential clinical markers for TA development and progression.Methods: Peripheral blood were collected from four TA patients requiring surgical management and four age-sex matched healthy donors. Single cell RNA sequencing (scRNA-seq) was adopted to explore the transcriptomic diversity and function of their PBMCs. ELISA, qPCR, and FACS were conducted to validate the results of the analysis.Results: A total of 29918 qualified cells were included for downstream analysis. Nine major cell types were confirmed, including CD14+ monocytes, CD8+ T cells, NK cells, CD4+ T cells, B cells, CD16+ monocytes, megakaryocytes, dendritic cells and plasmacytoid dendritic cells. CD14+ monocytes (50.0 vs. 39.3%, p &lt; 0.05) increased in TA patients, as validated by FACS results. TXNIP, AREG, THBS1, and CD163 increased in TA patients. ILs like IL-6, IL-6STP1, IL-6ST, IL-15, and IL-15RA increased in TA group.Conclusion: Transcriptome heterogeneities of PBMCs in TA patients requiring surgical management were revealed in the present study. In the patients with TA, CD14+ monocytes and gene expressions involved in oxidative stress were increased, indicating a new treatment and research direction in this field.
Interaction With the Extracellular Matrix Triggers Calcium Signaling in Trypanosoma cruzi Prior to Cell Invasion
Authors: Manchola Varón Nubia Carolina , dos Santos Guilherme Rodrigo R. M. , Colli Walter , Alves Maria Julia M. 
Published: October 2021

Trypanosoma cruzi, the etiological agent of Chagas disease in humans, infects a wide variety of vertebrates. Trypomastigotes, the parasite infective forms, invade mammalian cells by a still poorly understood mechanism. Adhesion of tissue culture- derived trypomastigotes to the extracellular matrix (ECM) prior to cell invasion has been shown to be a relevant part of the process. Changes in phosphorylation, S-nitrosylation, and nitration levels of proteins, in the late phase of the interaction (2 h), leading to the reprogramming of both trypomastigotes metabolism and the DNA binding profile of modified histones, were described by our group. Here, the involvement of calcium signaling at a very early phase of parasite interaction with ECM is described. Increments in the intracellular calcium concentrations during trypomastigotes-ECM interaction depends on the Ca2+ uptake from the extracellular medium, since it is inhibited by EGTA or Nifedipine, an inhibitor of the L-type voltage gated Ca2+ channels and sphingosine-dependent plasma membrane Ca2+ channel, but not by Vanadate, an inhibitor of the plasma membrane Ca2+-ATPase. Furthermore, Nifedipine inhibits the invasion of host cells by tissue culture- derived trypomastigotes in a dose-dependent manner, reaching 95% inhibition at 100 µM Nifedipine. These data indicate the importance of both Ca2+ uptake from the medium and parasite-ECM interaction for host-cell invasion. Previous treatment of ECM with protease abolishes the Ca2+ uptake, further reinforcing the possibility that these events may be connected. The mitochondrion plays a relevant role in Ca2+ homeostasis in trypomastigotes during their interaction with ECM, as shown by the increment of the intracellular Ca2+ concentration in the presence of Antimycin A, in contrast to other calcium homeostasis disruptors, such as Cyclopiazonic acid for endoplasmic reticulum and Bafilomycin A for acidocalcisome. Total phosphatase activity in the parasite decreases in the presence of Nifedipine, EGTA, and Okadaic acid, implying a role of calcium in the phosphorylation level of proteins that are interacting with the ECM in tissue culture- derived trypomastigotes. In summary, we describe here the increment of Ca2+ at an early phase of the trypomastigotes interaction with ECM, implicating both nifedipine-sensitive Ca2+ channels in the influx of Ca2+ and the mitochondrion as the relevant organelle in Ca2+ homeostasis. The data unravel a complex sequence of events prior to host cell invasion itself.
Sex Differences in Platelet Reactivity in Patients With ST-Elevation Myocardial Infarction: A Sub-Analysis of the ON-TIME 3 Trial
Authors: Tavenier Anne H. , Hermanides Renicus S. , Ottervanger Jan Paul , Belitser Svetlana V. , Klungel Olaf H. , Appelman Yolande , van Leeuwen Maarten A.H. , van 't Hof Arnoud W.J. 
Published: October 2021

Background: Fast and adequate platelet inhibition is one of the cornerstones in the treatment of patients with ST-elevation myocardial infarction (STEMI). The aim of this analysis is to examine sex differences in platelet inhibition in the acute treatment of STEMI patients.Methods: Platelet reactivity units (PRU) and ticagrelor plasma concentrations of all patients in the ON-TIME 3 were compared according to sex. All patients were pre-treated with crushed ticagrelor, aspirin and heparin. Both univariable and multivariable analyses were performed.Results: In this sub-analysis of the ON-TIME 3 trial, 195 STEMI patients, of which 58 female patients (29.7%) and 137 male patients (70.3%), were analyzed. PRU-values immediately post-PCI were not different in females [median 135 (IQR 47-228)] compared to males [160 (IQR 40-219), P = 0.92]. Ticagrelor plasma concentrations were higher in the females at the start of primary PCI [141 ng/mL (IQR 25-491) vs. 76 ng/mL (IQR 15-245), P = 0.049] and at 6 hours post-primary PCI [495 ng/mL (IQR 283-661) vs. 321 ng/mL (IQR 196-537), P = 0.001] compared to males. However, immediately post-primary PCI and at 1-hour post-primary PCI no significant differences in ticagrelor concentrations were seen between sexes. In multivariable analysis, sex was significantly associated with ticagrelor concentration (P = 0.04), but not with PRU (P = 0.93).Conclusion: Effective platelet inhibition reached by crushed ticagrelor in STEMI patients was similar in both sexes. Females had similar or even higher ticagrelor plasma concentrations up to 6 hours post-primary PCI compared with males.
Similar Inflammatory Biomarkers Reflect Different Platelet Reactivity in Percutaneous Coronary Intervention Patients Treated With Clopidogrel: A Large-Sample Study From China
Authors: Li Jiawen , Yuan Deshan , Jiang Lin , Tang Xiaofang , Xu Jingjing , Song Ying , Chen Jue , Qiao Shubin , Yang Yuejin , Gao Runlin , Xu Bo , Yuan Jinqing , Zhao Xueyan 
Published: October 2021

Background: Platelet reactivity is closely associated with adverse events in percutaneous coronary intervention (PCI) patients. Inflammation plays a crucial role in the development of coronary heart disease (CHD).Aim: To investigate the association of inflammatory biomarkers such as leukocyte count and high-sensitivity C reactive proteins (hs-CRP) with platelet reactivity in PCI patients treated with clopidogrel.Method: We examined 10,724 consecutive PCI patients in Fuwai hospital from January 2013 to December 2013. High on-treatment platelet reactivity (HTPR) was defined as adenosine diphosphate (ADP)-induced platelet maximum amplitude [MA(ADP)] of thromboelastogram (TEG) &gt; 47 mm, and low on-treatment platelet reactivity (LTPR) MA(ADP) &lt; 31 mm.Results: Finally, 6,772 PCI patients treated with clopidogrel who had the results of postoperative TEG were enrolled. Among them, 2,070 (30.57%) presented HTPR and 2,568 (37.92%) presented LTPR. As for LTPR, multivariate logistic regression showed that leukocyte count (OR: 1.153, 95% CI 1.117–1.191) and hs-CRP (OR: 0.920, 95% CI 0.905–0.936) were independent predictors, along with diabetes mellites, hemoglobin, platelet count and glucose. As for HTPR, multivariate logistic regression showed that leukocyte count (OR: 0.885, 95% CI 0.854–0.917) and hs-CRP (OR: 1.094, 95% CI 1.077–1.112) were independent predictors, along with sex, hemoglobin, platelet count and glucose.Conclusions: This was the first large real-world study reporting that both leukocyte count and hs-CRP were the independent factors for platelet reactivity in PCI populations treated with clopidogrel, among which higher leukocyte count was associated with more LTPR while higher hs-CRP was associated with more HTPR, providing new insights on individualized antiplatelet therapy.
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children
Authors: Villhauer Alissa , Lynch David , Postler Taylor , Dawson Deborah , Drake David 
Published: October 2021

Aim: The purpose of this study was to investigate the presence of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus species in an American Indian population displaying a high incidence of severe early childhood caries (S-ECC) and to explore the genotypic diversity and fidelity of transmission of S. sobrinus in this population.Methods: We report here on the microbial profiles of 71 children compiled from birth to 36 months of age and initial exploration of genotypic diversity in a subset of 40 mother/child dyads. Whole mouth plaque samples were collected from mother/child dyads in a Northern Plains American Indian cohort. Mutans streptococci (MS) and Lactobacillus counts were recorded and presence/absence of S. mutans, S. sobrinus, and Lactobacillus was noted for each child. S. sobrinus was isolated and genotyped via arbitrarily primed-polymerase chain reaction (AP-PCR).Results: The children in this study were divided into caries active (CA) and caries free (CF) groups. S. mutans, S. sobrinus, and Lactobacillus species were detected more frequently in CA groups. Distribution of MS and Lactobacillus counts was significantly different between the two groups, as was the distribution of three species colonization profiles. Twelve S. sobrinus genotypes were detected in the subset of mother/child dyads. Individual participants displayed a range of 0–3 distinct genotypes while mother/child pairs displayed a range of 1–5 genotypes. Eighty-three percent of children shared at least one genotype with his/her mother.Conclusion: Caries active children in this population display different microbial profiles than the caries free children in this cohort. A small number of S. sobrinus genotypes appear to be very common in this population. A high rate of vertical transmission of S. sobrinus from mother to child is seen in these mother/child dyads.
Development and Evaluation of a Fusion Polyprotein Based on HspX and Other Antigen Sequences for the Serodiagnosis of Tuberculosis
Authors: Zhou Fangbin , Xu Xindong , Cui Xiaobing , Pan Weiqing 
Published: October 2021

BackgroundThe lack of suitable diagnostic tools contributes to the high prevalence of tuberculosis (TB) worldwide. Serological tests, based on multiple target antigens, represent an attractive option for diagnosis of this disease due to their rapidity, convenience, and low cost.MethodsMeasures to reduce non-specific reactions and thereby improve the specificity of serological tests were investigated, including blocking antibodies against common bacteria in serum samples and synthesizing polypeptides covering non-conserved dominant B-cell epitopes of antigens. In addition, a fusion polyprotein containing HspX and eight other antigen sequences was constructed and expressed to increase overall sensitivity of the tests.ResultsInclusion of Escherichia coli lysate partially increased the specificity of the serological tests, while synthesis and inclusion of peptides containing non-conserved sequences of TB antigens as well as dominant B-cell epitopes reduced non-specific reactions without a decrease in sensitivity of the tests. A polyprotein fusing HspX and eight other antigen sequences was constructed and displayed 60.2% sensitivity, which was higher than that of HspX and the other individual antigen segments. Moreover, the specificity of the polyprotein was 93.8%, which was not significantly decreased when compared with HspX and the other individual antigen segments.ConclusionsThe roles of the fusion polyprotein in the humoral immune response against TB infection were demonstrated and provide a potential novel approach for the development of TB diagnostics.
From Cellular Infiltration Assessment to a Functional Gene Set-Based Prognostic Model for Breast Cancer
Authors: Li Huamei , Huang Yiting , Sharma Amit , Ming Wenglong , Luo Kun , Gu Zhongze , Sun Xiao , Liu Hongde 
Published: October 2021

BackgroundCancer heterogeneity is a major challenge in clinical practice, and to some extent, the varying combinations of different cell types and their cross-talk with tumor cells that modulate the tumor microenvironment (TME) are thought to be responsible. Despite recent methodological advances in cancer, a reliable and robust model that could effectively investigate heterogeneity with direct prognostic/diagnostic clinical application remained elusive.ResultsTo investigate cancer heterogeneity, we took advantage of single-cell transcriptome data and constructed the first indication- and cell type-specific reference gene expression profile (RGEP) for breast cancer (BC) that can accurately predict the cellular infiltration. By utilizing the BC-specific RGEP combined with a proven deconvolution model (LinDeconSeq), we were able to determine the intrinsic gene expression of 15 cell types in BC tissues. Besides identifying significant differences in cellular proportions between molecular subtypes, we also evaluated the varying degree of immune cell infiltration (basal-like subtype: highest; Her2 subtype: lowest) across all available TCGA-BRCA cohorts. By converting the cellular proportions into functional gene sets, we further developed a 24 functional gene set-based prognostic model that can effectively discriminate the overall survival (P = 5.9 × 10−33, n = 1091, TCGA-BRCA cohort) and therapeutic response (chemotherapy and immunotherapy) (P = 6.5 × 10−3, n = 348, IMvigor210 cohort) in the tumor patients.ConclusionsHerein, we have developed a highly reliable BC-RGEP that adequately annotates different cell types and estimates the cellular infiltration. Of importance, the functional gene set-based prognostic model that we have introduced here showed a great ability to screen patients based on their therapeutic response. On a broader perspective, we provide a perspective to generate similar models in other cancer types to identify shared factors that drives cancer heterogeneity.
Association of Frailty With the Risk of Mortality and Resource Utilization in Elderly Patients in Intensive Care Units: A Meta-Analysis
Authors: Xia Feiping , Zhang Jing , Meng Shanshan , Qiu Haibo , Guo Fengmei 
Published: October 2021

Background:The associations of frailty with the risk of mortality and resource utilization in the elderly patients admitted to intensive care unit (ICU) remain unclear. To address these issues, we performed a meta-analysis to determine whether frailty is associated with adverse outcomes and increased resource utilization in elderly patients admitted to the ICU.Methods:We searched PubMed, EMBASE, ScienceDirect, and Cochrane Central Register of Controlled Trials through August 2021 to identify the relevant studies that investigated frailty in elderly (≥ 65 years old) patients admitted to an ICU and compared outcomes and resource utilization between frail and non-frail patients. The primary outcome was mortality. We also investigated the prevalence of frailty and the impact of frailty on the health resource utilization, such as hospital length of stay (LOS) and resource utilization of ICU.Results:A total of 13 observational studies enrolling 64,279 participants (28,951 frail and 35,328 non-frail) were finally included. Frailty was associated with an increased risk of short-term mortality (10 studies, relative risk [RR]: 1.70; 95% CI: 1.45–1.98), in-hospital mortality (five studies, RR: 1.73; 95% CI: 1.55–1.93), and long-term mortality (six studies, RR: 1.86; 95% CI: 1.44–2.42). Subgroup analysis showed that retrospective studies identified a stronger correlation between frailty and hospital LOS (three studies, MD 1.14 d; 95% CI: 0.92–1.36).Conclusions:Frailty is common in the elderly patients admitted to ICU, and is associated with increased mortality and prolonged hospital LOS.Trial registration:This study was registered in the PROSPERO database (CRD42020207242).