bluuebunny/crossref_metadata_2025_split huggingface dataset:
An improved ChEC-seq method accurately maps the genome-wide binding of transcription coactivators and sequence-specific transcription factors
Authors: Donczew Rafal , Lalou Amélia , Devys Didier , Tora Laszlo , Hahn Steven 
Published: February 2021

AbstractMittal and colleagues have raised questions about mapping transcription factor locations on DNA using the MNase-based ChEC-seq method (Mittal et al., 2021). Partly due to this concern, we modified the experimental conditions of the MNase cleavage step and subsequent computational analyses, resulting in more stringent conditions for mapping protein-DNA interactions (Donczew et al., 2020). The revised method (dx.doi.org/10.17504/protocols.io.bizgkf3w) answers questions raised by Mittal et al. and, without changing earlier conclusions, identified widespread promoter binding of the transcription coactivators TFIID and SAGA at active genes. The revised method is also suitable for accurately mapping the genome-wide locations of DNA sequence-specific transcription factors.
Single-cell Visualization and Quantification of Trace Metals in Chlamydomonas Lysosome-Related Organelles
Authors: Schmollinger Stefan , Chen Si , Strenkert Daniela , Hui Colleen , Ralle Martina , Merchant Sabeeha S. 
Published: February 2021

AbstractThe acidocalcisome is an acidic organelle in the cytosol of eukaryotes, defined by its low pH and high calcium and polyphosphate content. It is visualized as an electron-dense object by transmission electron microscopy (TEM) or described with mass-spectrometry (MS)-based imaging techniques or multimodal X-ray fluorescence microscopy (XFM) based on its unique elemental composition. Compared to MS-based imaging techniques, XFM offers the advantage of absolute quantification of trace metal content, since sectioning of the cell is not required and metabolic states can be preserved rapidly by either vitrification or chemical fixation. We employed XFM inChlamydomonas reinhardtii, to determine single-cell and organelle trace metal quotas within algal cells in situations of trace metal over-accumulation (Fe, Cu). We found up to 70% of the cellular Cu and 80% of Fe sequestered in acidocalcisomes in these conditions, and identified two distinct populations of acidocalcisomes, defined by their unique trace elemental makeup. We utilized thevtc1mutant, defective in polyphosphate synthesis and failing to accumulate Ca to show that Fe sequestration is not dependent on either. Finally, quantitation of the Fe and Cu contents of individual cells and compartments via XFM, over a range of cellular metal quotas created by nutritional and genetic perturbations, indicated excellent correlation with bulk data from corresponding cell cultures, establishing a framework to distinguish the nutritional status of single cells.Significance statementTransition metals are of crucial importance for primary productivity; their scarcity limits crop yield in agriculture and carbon sequestration at global scale. Copper (Cu), iron (Fe) and manganese (Mn) are among the most important trace elements that enable the redox chemistry in oxygenic photosynthesis. The single-celled, eukaryotic green algaChlamydomonas reinhardtiiis a choice experimental system for studying trace metal homeostasis in the context of phototrophy, offering all the advantages of a classical microbial system with a well-characterized photosystem and trace metal metabolism machinery of relevance to plants. This project identifies and differentiates different trace metal storage sites in Chlamydomonas and uncovers the dynamics of trace metal storage and mobilization in situations of fluctuating resources.
Quantifying habitat and landscape effects on composition and structure of plant-pollinator networks in the US Northern Great Plains
Authors: Vilella-Arnizaut Isabela B. , Nottebrock Henning , Fenster Charles B. 
Published: February 2021

AbstractCommunity structure contributes to ecosystem persistence and stability. To understand the mechanisms underlying pollination and community stability of natural areas in a human influenced landscape, a better understanding of the interaction patterns between plants and pollinators in disturbed landscapes is needed. The Northern Great Plains still retain extensive tracts of remnant temperate grassland habitat within a matrix of varying land-uses. We used a network-based approach to quantify how temperate grassland attributes and landscape heterogeneity influence plant-pollinator community structure in natural habitats. We also quantified pollinator diversity and floral diversity to assess the functional role of temperate grassland attributes and the surrounding landscape on the composition of the plant-pollinator communities in natural habitats. We found that the amount of local nectar sugar and increased proportions of certain land-uses contribute to pollinator diversity that in turn influences the structure of interactions between plants and pollinators. Understanding the factors contributing to plant-pollinator network structure can guide management decisions to support resilient plant-pollinator communities and conserve the stability of pollination services.
Melanoma cells adopt features of both mesenchymal and amoeboid migration within confining channels
Authors: Gabbireddy Sairisheel R. , Chung Aram J. , Logue Jeremy S. 
Published: February 2021

AbstractFor metastasis to occur, cancer cells must traverse a range of tissue environments. In part, this is accomplished by cells adjusting their migration mode to one that is best suited to the environment. Melanoma cells have been shown to be particularly plastic, frequently using both mesenchymal and amoeboid (bleb-based) modes of migration. It has been demonstrated that 2D confinement will promote the transition from mesenchymal to bleb-based migration. However, if melanoma cells similarly transition to bleb-based migration in response to 3D confinement, such as within narrow channels, is unknown. Here, using micro-fabricated channels, we demonstrate that melanoma cells adopt features of both mesenchymal and bleb-based migration. In narrow (8 μm; height and width) channels coated with fibronectin, ~50% of melanoma cells were found to use either mesenchymal or bleb-based migration modes. In contrast, the inhibition of Src family kinases or coating channels with BSA, completely eliminated any features of mesenchymal migration. Detailed comparisons of migration parameters revealed that blebbing cells, particularly in the absence of adhesions, were faster than mesenchymal cells. In contrast to what has been previously shown under conditions of 2D confinement, pharmacologically inhibiting Arp2/3 promoted a fast filopodial-based mode of migration. Accordingly, we report that melanoma cells adopt a unique range of phenotypes under conditions of 3D confinement.
Adaptive sensor fusion labeling framework for hand pose recognition in robot teleoperation
Authors: Qi Wen , Liu Xiaorui , Zhang Longbin , Wu Lunan , Zang Wenchuan , Su Hang 
Published: February 2021

Purpose
The purpose of this paper is to mainly center on the touchless interaction between humans and robots in the real world. The accuracy of hand pose identification and stable operation in a non-stationary environment is the main challenge, especially in multiple sensors conditions. To guarantee the human-machine interaction system’s performance with a high recognition rate and lower computational time, an adaptive sensor fusion labeling framework should be considered in surgery robot teleoperation.

Design/methodology/approach
In this paper, a hand pose estimation model is proposed consisting of automatic labeling and classified based on a deep convolutional neural networks (DCNN) structure. Subsequently, an adaptive sensor fusion methodology is proposed for hand pose estimation with two leap motions. The sensor fusion system is implemented to process depth data and electromyography signals capturing from Myo Armband and leap motion, respectively. The developed adaptive methodology can perform stable and continuous hand position estimation even when a single sensor is unable to detect a hand.

Findings
The proposed adaptive sensor fusion method is verified with various experiments in six degrees of freedom in space. The results showed that the clustering model acquires the highest clustering accuracy (96.31%) than other methods, which can be regarded as real gestures. Moreover, the DCNN classifier gets the highest performance (88.47% accuracy and lowest computational time) than other methods.

Originality/value
This study can provide theoretical and engineering guidance for hand pose recognition in surgery robot teleoperation and design a new deep learning model for accuracy enhancement.
Spirituality, happiness and auditors' commitment: an interbeing perspective
Authors: Efferin Sujoko , Hutomo Christopher Christian 
Published: February 2021

PurposeThis study attempts to explore the meaning and implication of spirituality in an accounting firm by using a Buddhist perspective of interbeing. It explains how the happiness of individuals (auditors, partners, clients and auditor family members); organisational performance and growth and auditors' commitment are interconnected and impermanent.Design/methodology/approachThis study employed an interpretive case study in an Indonesian accounting firm. The researchers explored the collective and individual feelings, thoughts, actions and experiences of the firm's actors. The data collection methods were interviews, participant observations and documentary analysis.FindingsLeadership plays a major role in cultivating spirituality in an accounting firm. The spirituality increases auditors' commitment, (conditional) happiness and performance resulting in client satisfaction and the firm's growth. From an interbeing perspective, partners, auditors and clients are interconnected and impermanent. A firm's growth creates a growing sense of unhappiness due to the diminishing of auditors' comfort zone. Spirituality in the workplace can only engender conditional happiness and organisational commitment that offset the importance of material rewards and career prospects. To reach ultimate (unconditional) happiness, one requires a continuous spiritual development.Research limitations/implicationsThe insights gained from this study need enrichment from cases in different contexts, e.g. multinational firms with members from different countries and cultures.Originality/valueThis study develops the discourse of emancipation in the accounting literature by taking into account spirituality and happiness.
Management controls and crisis: evidence from the banking sector
Authors: Rikhardsson Pall , Rohde Carsten , Christensen Leif , Batt Catherine E. 
Published: February 2021

PurposeThis paper investigates the use of management controls when environmental uncertainty and hostility increase abruptly. Specifically, it explores this in the context of the 2008 financial crisis in six banks located in two countries.Design/methodology/approachThe paper is based on 26 qualitative interviews with selected managers employed by the six banks. Eight interview guides were developed based on the typology of controls in Malmi and Brown (2008). Respondents explained which changes in management controls occurred after the crisis.FindingsBoth organic and mechanistic management controls were mobilized at the same time to deal with the change. The use of controls played three main roles: (1) guide and control behavior, (2) change internal and external perceptions and (3) discharge accountability. Finally, control use during a crisis evolves as individual managers design and implement controls. There is no “grand design” rationally guiding the design of the overall system of controls.Originality/valueThe use of management controls in dealing with an increase in uncertainty and hostility cannot be labeled either organic or mechanistic, but will depend on the specific type of change in environmental characteristics. Management controls evolve by interaction with outside actors, as well as internal techniques.
Pathway to care in co-occurring disorder and substance use disorder: an exploratory, cross-sectional study from India
Authors: Tripathi Richa , Singh Shalini , Sarkar Siddharth , Lal Rakesh , Balhara Yatan Pal Singh 
Published: February 2021

PurposeThere is a paucity of comparative literature on pathway to care among patients with co-occurring disorders and those with only substance use disorders. This paper aims to compare the pathways to care among patients with co-occurring disorder and those with only substance use disorders.Design/methodology/approachA cross-sectional observational study was carried out on male treatment seekers at a tertiary care substance use disorder treatment center in India. Participants were recruited in two groups, those with co-occurring psychiatric and substance use disorders and those with only substance use disorders. The two groups were matched for age and socio-economic status.FindingsA total of 189 subjects with co-occurring psychiatric and substance use disorders and 197 subjects with substance use disorders only were recruited. Psychiatric services were the most common first point of care. However, a larger proportion of the subjects in the co-occurring disorder group received the first care from faith healers, while a greater proportion received first care from the therapeutic communities in substance use disorder only group. Initial care was sought mostly following suggestion from the family members in both the groups. The time to treatment for substance use disorders did not differ between the two groups, though the treatment seeking for substance use disorder was more delayed than that of psychiatric disorder in the co-occurring disorder group.Research limitations/implicationsThe findings shed light on the pathway of care followed in India and is a matter of further research.Practical implicationsExpansion of services and dissemination of information about psychiatric disorders and substance use disorders can provide timely care to patients with substance use disorders and co-occurring disorders.Social implicationsThe findings have a social implication as well. More awareness is needed currently in India for timely treatment of dual disorders.Originality/valueThe paper is an original research by the authors. The data were collected from the participants who reported to the dual diagnosis clinic. The findings are important as they tell us about the current understanding of dual diagnosis by the general public.
Research on influencing factors of fuel tank flammability exposure time for transport aircraft
Authors: Ruihua Zhang , Liu Weihua , Liu Wenyi 
Published: February 2021

Purpose
The assessment of fuel tank flammability exposure time for transport aircraft is one of the indispensable links in the airworthiness certification process. According to published literature, many factors can affect the flammability exposure time, while systematic analysis and calculations addressing these factors are in shortage.

Design/methodology/approach
Based on the requirements for airworthiness certification of domestic large aircraft, the fuel tank flammability exposure time of transport aircraft is calculated with the Monte Carlo evaluation model specified by Federal Aviation Administration. Meanwhile, the influence of each input parameter on the flammability exposure time is obtained by taking user input parameters in the model as independent variables and freezing other factors at the same time. The significance degree of the influence of each factor is discussed by the orthogonal test method. Subsequently, the interaction between the input parameters is studied by response surface method, and a multiple linear regression method is used to establish the functional relationship between the flammability exposure time and the influence parameters.

Findings
Research studies show that among the many factors that affect the flammability exposure time, the cruising Mach number, the equilibrium temperature difference and the maximum range are more significant and much attention should be paid to in the airworthiness certification; although there are interactions among various factors, they have different influence on the flammability exposure time, among which the interactions between maximum range and equilibrium temperature difference are the most significant compared with others; established by applying multiple linear regression equation and based on the test data of response surface method, the functional relationship between flammability exposure time and influence parameters is of sufficient reliability and can be used for preliminary prediction of fuel tank flammability exposure time for transport aircraft.

Originality/value
The research achievements of this paper can provide much useful reference for the certification of domestic large aircraft.
Glasgow anxiety scale for people with an intellectual disability (GAS-ID): validation for Spanish population
Authors: Núñez-Polo Mercedes Hernández , Alonso-Rodríguez María-Concepción , Dolado Ana , Ayuso-Mateos José-Luis , Martorell Almudena 
Published: February 2021

Purpose
Anxiety disorder is a common mental health problem in people with intellectual disabilities. Although this affects their quality of life, in Spain, there are no validated tools to evaluate the symptomatology of anxiety in people with intellectual disabilities. The aim of this study is to validate the Glasgow Anxiety Scale for people with an intellectual disability (GAS-ID) in the Spanish population.

Design/methodology/approach
The Spanish version of the GAS-ID was produced by back translation and was administered to 120 adults with intellectual disabilities. The psychometric analyses included internal consistency using the Cronbach’s alpha coefficient, inter-rater and test-retest reliability were determined using intra class correlation and Pearson correlation coefficients and, finally, factor analysis with Varimax rotation to confirm the construct validity of the questionnaire.

Findings
Cronbach’s alpha was 0.86 for the overall questionnaire. The intraclass correlation coefficient showed a good level of agreement in both test-retest (0.90) and inter-rater (0.91) analysis and the Pearson correlation showed a good significance in all dimensions and in the total scale. Varimax rotation factor analysis revealed four well-defined factors.

Originality/value
The GAS-ID is a feasible and reliable instrument for assessing anxiety symptoms in adults with mild and moderate intellectual disabilities, offering better diagnoses and therefore a more accurate treatment for the Spanish population with intellectual disabilities.
Social media usage in business decision-making: the case of Malaysian small hospitality organisations
Authors: Siti-Nabiha A.K. , Nordin Norfarah , Poh Boon Kar 
Published: February 2021

PurposeThe purpose of this paper was to examine how small- and medium-sized hospitality organisations engage with social media and how social media data are used by their managers to inform business decisions.Design/methodology/approachA qualitative approach was used in this research in which interviews were conducted with top management, comprising the owners/directors and other key managers from small- and medium-sized organisations based in Penang, Malaysia. Fan and Gordon's (2014) categorisation of the social media data analysis process and Simon's (1995) concept of the interactive and diagnostic usage of data were used in the analysis of data.FindingsThe managers of small- and medium-sized hospitality organisations engage with social media for customer relationship management and the understanding of key main competitors. Social media is used to understand, build and manage relationships with current and potential customers; these activities are also linked to actions taken to protect a company's reputation. Even though, for the companies concerned, data gathering is still at the capture stage with no formal procedures and processes in place, the data are utilised in an interactive way to inform two areas’ major business decisions-making, i.e. those related to pricing and promotion and the strategic formulation and reorientation of the business.Research limitations/implicationsThe respondents of this study were mainly from smaller hospitality organisations. Hence, the insights gained are limited to the context of smaller hotels.Originality/valueA significant number of social media studies within the hospitality sector have focussed on marketing aspects. This study explored the wider use of social media in the case of smaller hospitality organisations and how they compete and position themselves in the competitive hospitality industry.
Building a yardstick–a benchmark framework for assessing higher education management institutions
Authors: Palaniappan Umayal , Suganthi L. , Shagirbasha Shameem 
Published: February 2021

PurposeHigher education management institutions play a vibrant role in imparting managerial skills to the students to face the corporate world. Performance evaluation of such institutions is mandate to ensure the outcome quality. To establish this, the present research explored the critical performance indicators of management institutions using the balanced scorecard (BSC) approach.Design/methodology/approachThis research explored the critical performance indicators of public, private and standalone management institutions in India. Data were collected from the representative sample of all the stakeholders in those management institutions. A specific vision was created and a systematic procedure was employed to arrive at the objectives, measures and metrics of the scorecard specific to the vision. Confirmatory factor analysis was used to perform analysis on the collected data. For the objectives and measures that evolved from confirmatory factor analysis, metrics were formulated based on the expert opinion.FindingsThe study resulted in 16 objectives, 46 measures and 54 metrics encompassing all the four perspectives of BSC. This paper has contributed a concrete, concise, comprehensive and context specific framework.Research limitations/implicationsThe nature of the BSC framework paves the way for continuous assessment and eventually helps the institutions to attain sustainable growth. This research contributes to the literature of balanced scorecard and also to the performance assessment of the management institutions.Originality/valueBSC-based benchmarking is a unique contribution to the academia of management education to precisely measure the performance of institutions. The model comprehensively includes the indicators from all the perspectives of stakeholders in terms of objectives, measures and metrics, thus proposing a holistic assessment.