bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-house Reverse transcriptase polymerase chain reaction for detection of SARS-CoV2 with increased Sensitivity
Authors: Kalita Manash Jyoti , Dutta Kalpajit , Hazarika Gautam , Dutta Ridip , Kalita Simanta , Das Partha Pratim , Sarma Manash P , Banu Sofia , Idris Md. Ghaznavi , Talukdar Anjan Jyoti , Dutta Sangitanjan , Sharma Ajanta , Medhi Subhash 
Published: April 2021

Abstract
Background:With the increasing COVID-19 infection worldwide, economization of the existing RT-PCR based detection assay becomes the need of the hour. Methods: An assessment of optimal PCR conditions for simultaneous amplification for E, S and RdRp gene of SARS-CoV-2 has been made using both fast traditional and multiplex real time PCR using same primer sets. All variables of practical value were studied by amplifying known target-sequences from ten-fold dilutions of archived positive samples of COVID-19. Results: The designed primers for amplification of E, S and RdRp gene of SARS-Cov-2 in single tube Multiplex PCR amplifications have shown efficient amplification of the target region in 37 minutes using thermal cyclers and 169 minutes with HRM based Real time detection using SYBR green master mix, over a wide range of template concentration, and the results were in good concordance with the commercially available detection kits. Conclusion: This fast HRM based Real time multiplex PCR with SYBR green approach offers rapid and sensitive detection of SARS-CoV-2 in a cost effective manner apart from the added advantage of primer pair’s compatibility for use in Traditional multiplex PCR, which offers extended applicability of the assay protocol in resource limited setting.
Effects of Half-Dose and Full-Dose GnRH Antagonists on IVF-ET Outcomes: A Retrospective Study
Authors: Zhao Yingge , Lian Fang , Xiang Shan , Yu Yi , Pang Conghui , Qiu Yue 
Published: April 2021

Abstract
BackgroundGonadotropin-releasing hormone antagonist(GnRH-ant) has been shown to have a negative effect on endometrial receptivity. Therefore, the use of GnRH-ant dose as small as possible during controlled ovarian stimulation(COS) may has an impact on improving endometrial receptivity and pregnancy rate. However, the GnRH-ant dose is relatively flexible and there is no fixed requirement for guidance. In this retrospective study, we tried to study the effects of half-dose or full-dose GnRH-ant on IVF-ET outcomes.MethodsOf the 316 cycles for 314 patients analyzed in this study, 149 received half-dose GnRH-ant(Group1) and 167 received full-dose GnRH-ant(Group2). According to age and BMI, the two groups were divided into four subgroups. Age subgroups, they were divided into age≤35(subgroupA)and age>35(subgroupB): 180 cycles in subgroup A(107 cycles in subgroupA1,73 cycles in subgroupA2), 136 cycles in subgroup B(42 cycles in subgroup B1,94 cycles in subgroupB2). BMI subgroups, they were divided into BMI<25 (subgroupC)and BMI≥25 (subgroupD):208 cycles in subgroupC(94 cycles in subgroup C1,114 cycles in subgroupC2), 108 cycles in subgroupD (55 cycles in subgroupD1,53 cycles in subgroupD2).ResultsNeither fertilized oocytes and the number of superior-quality embryos nor clinical pregnancy rate and live production rate significantly differed between the two groups. However, the number of retrieved oocytes and available embryos were significantly larger in Group 1 than in Group 2 (8.17±4.10vs.7.07±4.05,2.96±2.03vs.2.52±1.62, respectively,p＜0.05). Indicators in each age subgroups showed no statistical significance.However, in BMI subgroups, neither fertilized oocytes, available embryos and the number of superior-quality embryos nor live production rate significantly differed between the four subgroups. The number of retrieved oocytes was higher in subgroupC1 than in subgroupC2 (8.24±4.04vs.6.83±3.92,p < 0.05), In addition the clinical pregnancy rate was slightly higher in subgroupD1 than in subgroupD2(45.45vs.24.53%,P< 0.05). ConclusionsThe results showed that half-dose GnRH-ant was as effective as full-dose GnRH-ant for most patients. And patients with BMI≥25 may be more suitable for half-dose GnRH-ant. This retrospective analysis and the small sample size are the main limitations of this study, and a large sample RCT will be carried out in the future.Trial registration﻿Retrospectively registered
Four Decades of Intensifying Precipitation from Tropical Cyclones
Authors: Shearer Eric , Nguyen Phu , Gorooh Vesta Afzali , Hsu Kuolin , Sorooshian Soroosh 
Published: April 2021

Abstract
In this study, the Precipitation Estimates from Remotely Sensed Information using Artificial Neural Networks (PERSIANN)-Dynamic Infrared Rain-rate model (PDIR) product, using a consistently measured 40-year archive of satellite-measured cloud-top infrared temperature data with a spatiotemporal resolution of 0.04° and 3-hourly as forcing data, is used to investigate the trends in TC precipitation from 1980 to 2019 using robust linear fitting over multi-year moving averages and accumulations of TC precipitation volume and rates. Trend analysis identifies significantly increasing trends of TC precipitation across intensity classifications. The mean and upper tail precipitation rates in hurricanes are shown to be rapidly increasing, with the greatest increases found in the most extreme precipitation rates of the strongest hurricanes. Increases in TC precipitation over land across TC intensities were observed. Increased counts of precipitation-containing pixels across of all intensities per TC are robustly shown across all but the strongest TC categories. Lastly, TC properties are significantly correlated with climate oscillation values from the Atlantic Multidecadal Oscillation (AMO), North American Oscillation (NAO), and the Oceanic Nino Index (ONI) prior to the start of the hurricane season. These results suggest that an increase in TC precipitation can be observed and that the previous lack of consensus can be attributed to data with low spatiotemporal resolution, limited temporal extents, or limited coverage i.e., exclusively over land.
Construction of Cellulose/Carboxymethyl Chitosan Hydrogels for Potential Wound Dressing Application
Authors: Guo Yi , Zhao Chuanyin , Yan Chao , Cui Li 
Published: April 2021

Abstract
In this study, novel cellulose/carboxymethyl chitosan (CMCS) composite hydrogels were constructed by blending cellulose and CMCS in LiOH/urea aqueous solutions, and then cross-linking with epichlorohydrin. The structure and morphology of the composite hydrogels were characterized by Fourier transform infrared spectroscopy (FT-IR), wide-angle X-ray diffraction (WXRD), thermo-gravimetric analysis (TGA), and scanning electron microscopy (SEM). The results revealed that the chemical cross-linking reaction between cellulose and CMCS occurred in the hydrogel, and CMCS contributed to the enhancement of pore size, whereas cellulose as a strong backbone in the hydrogel to support the pore wall. The mechanical strength of the composite hydrogels increased with the cellulose content, while the equilibrium swelling ratio and antibacterial activity increased with the CMCS content. The composite hydrogels had no cytotoxicity towards L929 cells, suggesting good biocompatibility. All these results indicate that cellulose/CMCS composite hydrogels can be effectively used as a material in wound dressing.
Robot-Assisted Radical Prostatectomy in Patients With Clinically High-Risk Prostate Cancer: Surgical Feasibility and Long-Term Functional and Oncologic Outcomes
Authors: Lee Yong Seong , Shin Tae Young 
Published: April 2021

Abstract
Background

Robot-assisted radical prostatectomy (RARP) is an acceptable procedure for localized prostate cancer. However, RARP has not been offered to patients with high-risk prostate cancer. We report long-term functional and oncologic outcomes of patients who underwent RARP for clinically high-risk prostate cancer and to assess the role of RARP in patients with high-risk prostate cancer.
Methods

This study included 90 patients with high-risk prostate cancer according to the D'Amico criteria who underwent RARP between January 2014 and December 2019. High risk was based on the presence of a clinical stage of ≥ T2c, a pretreatment prostate-specific antigen level > 20 ng/mL, or a biopsy Gleason score ≥ 8. Functional outcomes including postoperative continence and potency were assessed at 1, 3, 6, and 12 months after RARP. Oncologic outcomes comprised positive surgical margins (PSMs), biochemical recurrence (BCR), BCR-free survival, and clinical recurrence (CR)-free survival rates at 1 and 3 years.
Results

The median operative time was 185 (interquartile range [IQR], 140–250) minutes. Based on postoperative pathology, the rates of PSMs in the entire cohort and in those with stage pT2 disease were 27.8% and 8.9%, respectively. The continence and potency rates at 12 months were 87.8% and 56.7%, respectively. The BCR rate was 23.3%, and the median time to BCR was 10.5 (IQR, 3.5–26.9) months. The 1- and 3-year BCR-free survival rates were 91.5% and 85.5%, respectively, and the 1- and 3-year CR-free survival rates were 97.5% and 90.8%, respectively.
Conclusions

Most patients with clinically high-risk prostate cancer treated with RARP remained BCR-free and CR-free during the long-term follow-up. The optimal functional and oncologic outcomes indicating RARP as a safe and feasible approach in the present study should be confirmed in future studies.
The Analgesic Comparison of Supraclavicular and Interscalene Brachial Plexus Block for Shoulder Arthroscopy: A Meta-Analysis of Randomized Controlled Trials
Authors: Huang Liangku , Li Peng , Zhang Liang , Kang Guangming , Zhou Haizhen , Zhao Zandong 
Published: April 2021

Abstract
Introduction:

The analgesic comparison of supraclavicular versus interscalene brachial plexus block (SCBB versus ISBB) for pain management of shoulder arthroscopy remains controversial. We conduct a systematic review and meta-analysis to explore the influence of SCBB versus ISBB on the postoperative pain intensity of shoulder arthroscopy.
Methods

We have searched PubMed, EMbase, Web of science, EBSCO, and Cochrane library databases through March 2021 for randomized controlled trials (RCTs) assessing the effect of SCBB versus ISBB on pain control of shoulder arthroscopy. This meta-analysis is performed using the random-effect model.
Results

Six RCTs are included in the meta-analysis. Overall, compared with ISBB for shoulder arthroscopy, SCBB leads to similar pain scores at 24 h (SMD = 0.07; 95% CI=-0.14 to 0.28; P = 0.52) and additional analgesic requirement (SMD=-0.22; 95% CI=-0.52 to 0.09; P = 0.16), but results in increased onset time of block (SMD = 1.77; 95% CI = 0.21 to 3.34; P = 0.25), decreased incidence of horner’s syndrome (OR = 0.25; 95% CI = 0.10 to 0.64; P = 0.003) and adverse events (OR = 0.25; 95% CI = 0.11 to 0.58; P = 0.001).
Conclusions

SCBB demonstrated comparable pain control after shoulder arthroscopy compared to ISBB, with lower incidence of adverse events.
GIS-Based Flood Hazard Mapping in Itang District of the Gambella Region, Ethiopia
Authors: Tadesse Dame , Karuturi Venkata Suryabhagavan , Nedaw Dessie 
Published: April 2021

Abstract
Flood is one of the natural disasters that affect societies around the world. Every year, flood claims hundreds of human lives and causes damage to the global economy and environment. Consequently, the identification of flood-vulnerable areas is important for comprehensive flood risk management. This study aims to delineate flood hazard areas in Itang District of Gambella Region, Ethiopia, applying the Gumbel probability distribution and Analytical Hierarchy Process (AHP) method. Distribution models applied in the study involve the generalized extreme value, Gumbel, Log-Normal, and Log Pearson type III. The Gumbel distribution provides the best fit according to the extreme value analyses. After obtaining discharge level through the method, the region's flood inundation area was mapped at 5, 10, 25, 50, and 100-year recurrence intervals. The developed methodology processes six parameters, namely, slope, elevation, land-use/land-cover, rainfall, discharge, and soil. Each criterion was evaluated with the aid of AHP and mapped by GIS. Data were assorted into five suitability classes, viz., very high, high, moderate, low, and very low flooding areas, representing 29.7%, 27.8%, 18.5%, 12.7%, and 11.4%, respectively. The outcome is extremely useful for evacuation planning, damage assessment, and losses estimation, thereby minimizing the natural disaster's effect in the study area.
Preparation, Properties and Applications of the Hybrid Organic/Inorganic Nanocomposite Based on Nanoporous Carbon Matrix
Authors: MANSOUR NABIL BEN , Djeridi Wahid , Mir Lassaad El 
Published: April 2021

Abstract
Nanoporous carbon matrix was prepared by the sol-gel process from pyrogallol-formaldehyde (PF) mixtures in water using picric acid as catalyst. For the second sample, nickel oxide nanoparticles were added in the PF matrix to get a PF/NiO hybrid nanocomposite. The samples were heat treated at the pyrolysis temperature of 650°C for two hours. The obtained materials have been characterized by different techniques such as: X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), differential thermalanalysis (DTA),nitrogen adsorption-desorption isotherms, voltage-current characteristics, electrochemical measurementsand UV-Vis-IR absorption. The XRD analysis shows that PF matrix is amorphous while PF/NiO nanocomposite exhibited a metallic phase of nickel. The SEM and TEM images indicated the presence of nickel particles in the PF/NiO nanocomposite which are dispersed randomly in the carbon matrix. The adsorption-desorption of nitrogen revealed that the PF matrix is microporous and the PF/NiO nanocomposite is mesoporous. The adsorption capacities of carbon dioxide (CO2) and methane (CH4) show that the PF matrix has the highest adsorption at low pressures and the PF/NiO nanocomposite tends to adsorb gases better at high pressure. The V(I) characteristics showed that the PF/NiO nanocomposite can be considered as a smart material. Indeed, the characteristic behavior can be adjusted according to the maximum applied current. Electrochemical measurements have shown that the PF/NiO nanocomposite is very promising for the detection of non-enzymatic glucose. The absorption spectra showed that the addition of the NiO nanoparticles increased the disorder. The obtained materials are promoters for various applications such as gas storage, negatronic devices and glucose sensor.
Chronic Backpain Among Adolescents in Denmark: Socioeconomic Inequality and Trends 1991-2018
Authors: Holstein Bjørn E. , Damsgaard Mogens Trab , Madsen Katrine Rich , Pedersen Trine Pagh , Toftager Mette 
Published: April 2021

Abstract
Chronic backpain among adolescents is important because the prevalence and the burden of disability is high. Chronic backpain tracks into adulthood and is associated with several health problems. The objective was to study trends in the prevalence of chronic backpain among adolescents 1991-2018, to examine the association with socioeconomic status (SES) and whether this association changed over time. The study used data from eight comparable cross-sectional school-surveys of nationally representative samples of 11-15-year-olds in 1991, 1994, 1998, 2002, 2006, 2010, 2014 and 2018, the Danish arm of the international Health Behaviour in School-aged Children (HBSC) study. The participation rate was 88.0%, n=29,952. Chronic backpain was defined as self-reported backpain daily or several days a week during the last six months. The prevalence of chronic backpain was 11.1%, significantly increasing from 8.9% in 1991 to 11.7% in 2018. The OR for chronic backpain was 1.20 (1.10-1.31) in middle and 1.56 (1.41-1.73) in low compared to high OSC. Sensitivity analyses with two other cut-points for backpain frequency showed similar associations. Conclusion: Chronic backpain is common among adolescents and the prevalence increased from 1991 to 2918. The prevalence was highest in lower SES families. We recommend increased efforts to prevent chronic backpain.
Synergic Effect of Combined Therapy of Hyperbaric Oxygen and Adipose-derived Mesenchymal Stem Cells on Improving Neurological Function After Acute Traumatic Spinal Cord Injury in Rat
Authors: Yin Tsung-Cheng , Shao Pei-Lin , Chen Kuan-Hung , Lin Kun-Chen , Chiang John Y. , Sung Pei-Hsun , Wu Shun-Cheng , Li Yi-Chen , Yip Hon-Kan , Lee Mel S 
Published: April 2021

Abstract
Background: This study tested whether combined hyperbaric oxygen (HBO) and allogenic adipose-derived mesenchymal stem cells (ADMSCs) would be superior to either one for improving the neurological function in rat after acute traumatic spinal cord injury (TSCI) in rat. Methods and Results: Adult-male SD rats (n=40) were equally categorized into group 1 (sham-operated control), group 2 (TSCI), group 3 (TSCI + HBO for 1.5h/day for 14 consecutive days after TSCI), group 4 (TSCI + ADMSCs/1.2x106 cells by intravenous injection at 3h and days 1/2 after TSCI) and group 5 (TSCI + HBO + ADMSCs), euthanized and spinal-cord tissue was harvested by day 49 after TSCI. The result showed that the protein expressions of oxidative-stress (NOX-1/NOX-2), inflammatory-signaling (TLR-4/MyD88/IL-1ß/TNF-α/substance-p), cell-stress signaling (PI3K/p-AKT/p-mTOR) and the voltage gated sodium channel (Nav1.3/1.8/1.9) biomarkers were highest in group 2, lowest in group 1 and significantly lower in group 5 than in groups 3/4 (all p<0.0001), but they did not differ between groups 3/4. The spinal cord-damaged area, the cellular levels of inflammatory/DNA-damaged (CD68+/GFAP+/γ-H2AX+ cells), MAPK family biomarkers (p-P38/p-JNK/p-ERK1/2) and cellular expressions of voltage gated sodium channel (Nav.1.3, Nav.1.8 and Nav.1.9 in NF200+ cells) as well as the pain facilitated cellular expressions (p-P38+/peripherin+ cells, p-JNK+/peripherin+ cells, p-ERK/NF200+ cells) exhibited an identical pattern of inflammation, whereas the neurological integrity displayed an opposite pattern of inflammation among the groups (all p<0.0001). Conclusion: Combined HBO-ADMSCs therapy offered additional benefits for protecting the neurological architectural and functional integrity against acute TSCI.
Prolonged Hemiplegic Migraine Associated with Irreversible Neurological Deficit and Cortical Laminar Necrosis in a Patient with Patent Foramen Ovale ：A case report
Authors: Hu Yacen , Wang Zhiqin , Zhou Lin , Sun Qiying 
Published: April 2021

Abstract
Background: Aura symptoms of hemiplegic migraine (HM) usually resolve completely, permanent attack-related deficit and radiographic change are rare. Case presentation: We reported a HM case presented with progressively aggravated hemiplegic migraine episodes refractory to medication. He experienced two prolonged hemiplegic migraine attacks that led to irreversible visual impairment and cortical laminar necrosis (CLN) on brain MRI. Patent foramen ovale (PFO) was found on the patient. PFO closure resulted in a significant reduction of HM attacks. Conclusions: Prolonged hemiplegic migraine attack could result in irreversible neurological deficit with neuroimaging changes manifested as CLN. We recommend screening for PFO in patients with prolonged or intractable hemiplegic migraine, for that closure of PFO might alleviate the attacks thus preventing patient from disabling sequelae.
Comprehensive Analysis of a lncRNA-miRNA-mRNA Competing Endogenous RNA Network in Heart Failure
Authors: Luo Xiaoqian , Lu Weina , Jiang Rufang , Hu Jun , Chen Enjiang , Weng Meiling , Fu Qinghui 
Published: April 2021

Abstract
Background: Acute heart failure caused by progressive heart failure is a common disease in intensive care units (ICU). The growing incidence rate of heart failure and its high mortality rate result are very important sociosanitary problems. Therefore, it is important to identify the molecular mechanism by which heart failure occurs and to identify treatment for this mechanism. Recently, the mechanism of ceRNA has attracted increasing attention. The aim of the present study was to identify the candidate ceRNA network in the progression of heartfailure.Method: Microarray datasets GSE9128, GSE61741 and GSE77399 were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified, and function enrichment analyses were performed. The protein-protein interaction network (PPI) was constructed and identification of hub-genes was performed using STRING and Cytoscape. Furthermore, according to the ceRNA theory, network of ceRNA was constructed.Result: In the present study, based on the ceRNA theory and above series of analyses, network of ceRNA which include 7 mRNAs (BCL2A1, DUSP1, EGR1, MYC, NR4A2, PTGS2 and RAC2), 3 miRNAs (miR-20a, miR-129-59 and miR-185-5p) and 3 lncRNAs (GAS5, H19 and PCGEM1) were obtained.Conclusion: In conclusion, these findings can be used to carrying on further study to identify the important roles of the ceRNA, biological function, appropriate treatment targets and biomarkers in the progression of heart failure.
Women’s Experiences and Perceptions of Anxiety and Stress During The Perinatal Period: A Qualitative Evidence Synthesis
Authors: McCarthy Megan , Houghton Catherine , Matvienko-Sikar Karen 
Published: April 2021

Abstract
Background. The perinatal period, from pregnancy to the first year postpartum, is a transitional period that can result in anxiety and stress for some women. Perinatal anxiety and stress can adversely impact the physical and psychological health of women and children. Understanding women’s lived experiences of perinatal anxiety and stress is essential to better support women. The aim of this qualitative evidence synthesis was to examine women’s experiences and perceptions of, and barriers and facilitators to coping with, perinatal anxiety and stress.Methods. Databases CINAHL, EMBASE, MEDLINE, PsycINFO and Maternity and Infant Care were searched from inception to June 2020. Eligible studies included women who were pregnant or up to one year postpartum and examined women’s experiences of anxiety and/or stress during the perinatal period. Data were synthesised using thematic synthesis.Results. Of 20,318 identified articles, 13 studies met inclusion criteria and were included in this review. Five key themes emerged: Social support, women’s experiences of healthcare, social norms and expectations, factors that impact on coping and mother and baby’s health.Limitations. Studies were predominantly conducted in high-income countries.Conclusion. This review provided a comprehensive synthesis of perinatal anxiety and stress. Findings indicate that increased support for perinatal mental health in antenatal and postpartum care is needed. Addressing unrealistic expectations and conceptualisations of motherhood is also important to better support women. Enhancing women’s social support networks and provision of clear and consistent information are also essential to support women and minimise stress and anxiety in the perinatal period.