bluuebunny/crossref_metadata_2025_split huggingface dataset:
Living under threat: Will one of the last Pinna nobilis populations be able to survive?
Authors: Nebot‐Colomer Elisabet , Álvarez Elvira , Belando Maria Dolores , Deudero Salud , Catanese Gaetano , Bernardeau‐Esteller Jaime , García‐Muñoz Rocío , Ramos‐Segura Aranzazu , Ruiz Juan Manuel , Vázquez‐Luis Maite 
Published: November 2021

Abstract

The critically endangered fan mussel Pinna nobilis, endemic to the Mediterranean Sea, faces a drastic population decline all over the Mediterranean Sea owing to an infectious disease.
Coastal lagoons represent one of this species’ last reservoirs; thus, knowledge of the population conservation status in these ecosystems is essential.
An extensive monitoring programme was conducted during 2014, 2017 and 2019 in the Mar Menor lagoon, a highly eutrophicated lagoon from Spain. On average, 32,535 m2 have been surveyed each year to determine population densities, distributions and size structures.
The cumulative impacts produced by intensive agriculture activity around the lagoon, together with a catastrophic meteorological phenomenon that occurred in 2019, have triggered a significant decline in fan mussel population densities and in population mean shell sizes over the years. Additionally, some fan mussels were infected by the protozoan Haplosporidium pinnae.
Therefore, the current P. nobilis population from the Mar Menor is mainly composed of young individuals in specific areas that are facing an extreme situation. Urgent management and conservation actions focused on restoring the ecosystem and protecting P. nobilis individuals are urgently needed, together with reinforcement projects to increase the species’ stock in one of its last reservoirs.
Emergence of verbal responses using instructive feedback: A replication and extension
Authors: Tullis Christopher A. , Gibbs Ashley R. , Priester Jocelyn , Tillem Alix 
Published: October 2021

AbstractFew studies have investigated the potential of instructive feedback (IF) procedures to result in the emergence of novel‐related verbal responses. Furthermore, few studies have directly assessed the role self‐echoic behavior may have in IF procedures. The current replication and extension evaluated the effects of IF procedures during unmastered primary target trials on the emergence of novel verbal responses. All learners acquired primary, secondary, and related, but unpresented, verbal responses, and maintained responding for 2 months. These results extend the IF literature by providing evidence that IF may facilitate the emergence of novel verbal responses during skill acquisition.
The nexuses between transformational leadership and employee green organisational citizenship behaviour: Role of environmental attitude and green dedication
Authors: Khan Ali Nawaz , Khan Naseer Abbas 
Published: November 2021

AbstractOrganisations' green human resources, production processes and other processes would be better positioned to meet the rising challenge of global warming if organisations follow an extensive environmental sustainability and behavioural norms and strategy. In so doing, this article looks at the influence of transformational leadership on employee green dedication and organisational citizenship behaviour (OCB) via green dedication in two different studies from two different industries (construction and manufacturing). Study 1 aims to investigate the mediating role of green dedication in linking transformational leadership to employees' green OCB using cross‐sectional data. Study 2 used the same research model as study 1 but with the addition of a moderator (environmental attitude) to affect the impact of transformational leadership on employee green OCB through employee dedication using the time lag approach. In studies 1 and 2, we collected data in supervisor–employee dyad from construction firms and manufacturing enterprises, respectively. The findings of study 1 indicated that transformational leadership has a significant impact on employee green OCB. Green dedication was also found to significantly mediate the association between transformational leadership and employee green OCB. The findings of study 2 showed that environmental attitude moderated significantly the association between transformational leadership and green dedication. This article provides useful insights for environmental researchers, managers and policymakers to reform human resource practices to encourage green organisational citizenship behaviour at work.
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations
Authors: Yehl Christopher J. , Zydney Andrew L. 
Published: October 2021

AbstractHigh‐performance countercurrent membrane purification (HPCMP) has recently been presented as a new approach for protein separations, exploiting differences in diffusive transport across a semipermeable membrane to achieve high selectivity for protein separations. This study presents a set of design equations and diagrams that describe the tradeoff between the yield and purification factor in HPCMP processes in terms of two parametric variables: the diffusive membrane selectivity and the ratio of the draw to bulk solution flow rates. Conditions are identified that provide the high yields and purification factors of interest in bioprocessing. In addition, hydrodynamic models for solute transport were used to evaluate the selectivity as a function of the membrane pore size distribution for purely size‐based separations. Model calculations demonstrate that diffusive transport provides significantly greater selectivity than traditional pressure‐driven membrane separations for the same pore size distribution due to differences in hindered transport rates for diffusion and convection. These results provide a framework that can be used for the development of HPCMP processes for highly selective protein separations.
Direct Enzymatic Synthesis of Fatty Amines from Renewable Triglycerides and Oils
Authors: Citoler Joan , Finnigan William , Bevinakatti Han , Turner Nicholas J. 
Published: November 2021

AbstractFatty amines represent an important class of commodity chemicals which have broad applicability in different industries. The synthesis of fatty amines starts from renewable sources such as vegetable oils or animal fats, but the process has multiple drawbacks that compromise the overall effectiveness and efficiency of the synthesis. Herein, we report a proof‐of‐concept biocatalytic alternative towards the synthesis of primary fatty amines from renewable triglycerides and oils. By coupling a lipase with a carboxylic acid reductase (CAR) and a transaminase (TA), we have accomplished the direct synthesis of multiple medium and long chain primary fatty amines in one pot with analytical yields as high as 97 %. We have also performed a 75 mL preparative scale reaction for the synthesis of laurylamine from trilaurin, obtaining 73 % isolated yield.
Acid‐Catalyzed RNA‐Oligomerization from 3’,5’‐cGMP
Authors: Wunnava Sreekar , Dirscherl Christina F. , Výravský Jakub , Kovařík Aleš , Matyášek Roman , Šponer Jiří , Braun Dieter , Šponer Judit E. 
Published: November 2021

AbstractThe assembly of ancient informational polymers from nucleotide precursors is the central challenge of life's origin on our planet. Among the possible solutions, dry polymerization of 3’,5’‐cyclic guanosine monophosphate (3’,5’‐cGMP) has been proposed as a candidate to create oligonucleotides of 15–20 units in length. However, the reported sensitivity of the reaction to the presence of cations raised questions of whether this chemistry could be relevant in a geological context. The experiments in this study show that the presence of cations is not restrictive as long as the reaction is conducted in an acidic environment, in contrast to previous reports that suggested optimal conditions at pH 9.
Adenine Modification at C7 as a Viable Strategy to Potentiate the Antimalarial Activity of Quinolones
Authors: Kurian Jais , Kumari Varsha , Chaluvalappil Saheer V. , Anas Mohammad , Manhas Ashan , Kalluruttimmal Ramshad , Kumar Niti , Manheri Muraleedharan K. 
Published: October 2021

AbstractAlthough many quinolones have shown promise as potent antimalarials, their clinical development has been slow due to poor performance in vivo. Insights into structural modifications that can improve their therapeutic potential will be very valuable in this vibrant area of research. Our studies involving a library of quinolones which vary in substitution pattern at N1, C3, C6 and C7 positions have shown that the presence of adenine moiety at C7 can bring a noticeable improvement in activity compared to other heterocyclic groups at this location. The most potent compound emerged from this study showed IC50 values of 0.38 μM and 0.75 μM against chloroquine‐sensitive and ‐resistant (W2) strains, respectively. Docking analysis in the Qo site of cytochrome bc1 complex revealed the contribution of a key H‐bonding interaction from the adenine unit in target binding. This corroborates with compound‐induced loss of mitochondrial functions. These findings not only open avenues for further exploration of antimalarial potential of adenine‐modified quinolones, but also suggests broader opportunities during lead‐optimization against other antimalarial targets.
Parameter optimization design and performance analysis of filter‐integrated switched boost grid‐connected inverter
Authors: Liu Shuo , Zhou Xu , Li Jianlin , Yang Liyong 
Published: October 2021

AbstractThis paper proposes a filter‐integrated switched boost grid‐connected inverter by coupling switched boost network between inverter bridge and power grid, which can effectively solve the inherent resonance peak problem of LCL filter. Meanwhile, the proposed topology can realize boost function at AC side, which can effectively avoid the problem of excessive voltage stress caused by the boost at the DC side. Due to the design of the parameters in the control system will affect the effect of resonance spike damping, it will also affect the harmonic content of the grid‐connected current. Based on the stability margin and steady‐state error of the system, combined with the characteristics of the system regulator, the parameter selection area that meets the system requirements is drawn in this paper, and the optimization of the system parameter design method is realized. Finally, the function of the circuit is verified by building an experimental platform.
Solitary cutaneous metastasis from an ovarian high‐grade serous carcinoma at the initial presentation: Cytologic diagnosis of a rare manifestation
Authors: Kaur Amarjot , Gupta Parikshaa , Gupta Nalini , Rai Bhavana 
Published: October 2021

AbstractCutaneous metastasis can rarely be the first manifestation of visceral malignancies and is commonly seen in advanced‐stage malignancies. It is infrequently seen in patients with ovarian malignancies and may develop either late in the course of the disease or at the initial presentation. Such cases are often associated with poor prognosis, and a prompt, precise tissue diagnosis is essential for appropriate patient management and better clinical outcome. Herein, we present a case of cutaneous metastasis in a young woman with an undiagnosed abdominopelvic mass that was diagnosed as metastatic high‐grade serous carcinoma (HGSC) on fine‐needle aspiration cytology (FNAC) supplemented by immunocytochemistry (ICC) on the cell block. The index case documents a unique and rare metastatic presentation of ovarian HGSC, as non‐Sister Mary Joseph anterior abdominal wall nodule, at the initial presentation. Additionally, it highlights the utility of minimally‐invasive FNA combined with ICC in prompt and accurate preoperative diagnosis of an underlying ovarian malignancy.
Electrically driven microfluidic platforms for exosome manipulation and characterization
Authors: Diaz‐Armas Gladys G. , Cervantes‐Gonzalez Ana P. , Martinez‐Duarte Rodrigo , Perez‐Gonzalez Victor H. 
Published: October 2021

AbstractExosomes are small extracellular vesicles that can be obtained from several body fluids such as blood and urine. Since these vesicles can carry biomarkers and other cargo, they have application in healthcare diagnostics and therapeutics, such as liquid biopsies and drug delivery. Yet, their identification and separation from a sample remain challenging due to their high degree of heterogeneity and their co‐existence with other bioparticles. In this contribution, we review the state‐of‐the‐art on electrical techniques and methods to displace, selectively trap/isolate, and detect/characterize exosomes in microfluidic devices. Although there are many reviews focused on exosome separation using benchtop equipment, such as ultracentrifugation, there are limited reviews focusing on the use of electrical phenomena in microfluidic devices for exosome manipulation and detection. Here, we highlight contributions published during the past decade and present perspectives for this research field for the near future, outlining challenges to address in years to come.
Modelling of large wood export at a watershed scale
Authors: Komori Daisuke , Sukegawa Yuto , Chaithong Thapthai , Kazama So 
Published: October 2021

AbstractThis study proposed a quantitative model for analysing the mass balance of large wood and its export on an annual scale at a watershed scale. The study sites are five dam reservoir watersheds of the Kitakami River catchment located in the north‐eastern part of Japan. From analyses on 20 years patterns of actual large wood export to target dam reservoirs, (1) the annual 24‐h maximum rainfall would be a key factor for the annual large wood recruitment, and (2) large wood export characteristics on an annual scale would be based on two relationships. Based on the earlier findings, the model consists of two frameworks: the rainfall‐induced analytical shallow landslide model for the large wood recruitment, and the double storage function with the lumped hydrological method at a watershed scale for the large wood entrainment.The model was used to re‐analyse 20 years of actual large wood export. The Nash–Sutcliffe values were 0.7 or more, which indicated satisfactory results with high reproductivity of simulation, for large wood export estimated using this model for four among five dam reservoir watersheds. Namely, our results could demonstrate that the landslide or slope failures are a significant procedure of large wood export, and the characteristics of large wood export can be defined by two relationships, which are the direct export of large wood caused by an increase in large wood recruitment with the extreme rainfall event, and the baseflow of large wood, which is mainly old large wood recruitment stored at the slopes and in the stream.
Loss of glutamate transporter eaat2a leads to aberrant neuronal excitability, recurrent epileptic seizures, and basal hypoactivity
Authors: Hotz Adriana L. , Jamali Ahmed , Rieser Nicolas N. , Niklaus Stephanie , Aydin Ecem , Myren‐Svelstad Sverre , Lalla Laetitia , Jurisch‐Yaksi Nathalie , Yaksi Emre , Neuhauss Stephan C. F. 
Published: October 2021

AbstractAstroglial excitatory amino acid transporter 2 (EAAT2, GLT‐1, and SLC1A2) regulates the duration and extent of neuronal excitation by removing glutamate from the synaptic cleft. Hence, an impairment in EAAT2 function could lead to an imbalanced brain network excitability. Here, we investigated the functional alterations of neuronal and astroglial networks associated with the loss of function in the astroglia predominant eaat2a gene in zebrafish. We observed that eaat2a−/− mutant zebrafish larvae display recurrent spontaneous and light‐induced seizures in neurons and astroglia, which coincide with an abrupt increase in extracellular glutamate levels. In stark contrast to this hyperexcitability, basal neuronal and astroglial activity was surprisingly reduced in eaat2a−/− mutant animals, which manifested in decreased overall locomotion. Our results reveal an essential and mechanistic contribution of EAAT2a in balancing brain excitability, and its direct link to epileptic seizures.
Management of first‐line palliative chemotherapy for post‐treatment metastasis after gemcitabine plus cisplatin induction chemotherapy: Gemcitabine plus cisplatin and non‐gemcitabine plus cisplatin chemotherapy
Authors: Yang Zhen‐Chong , Du Chao‐Chao , Liu Ting , Liu Li‐Ting , Chen Qiu‐Yan , Guo Shan‐Shan , Mai Hai‐Qiang 
Published: October 2021

AbstractBackgroundTo evaluate whether patients with post‐treatment metastasis are suitable for GP first‐line palliative chemotherapy (PCT) after undergoing GP IC.MethodsSeven hundred and forty‐six patients with post‐treatment metastasis after undergoing GP IC were eligible. Survival outcomes were compared.ResultsSignificant differences in survival rates were observed between patients treated with GP and non‐GP chemotherapy (2‐year progression‐free survival [PFS]: 0.7% vs. 9.7%). We investigated survival outcomes of patients treated with GP PCT within 2 years after undergoing GP IC, treated with GP PCT 2 years after undergoing GP IC, and those of non‐GP PCT patients (2‐year PFS: 0.0%, 2.3%, 9.7%). However, there was no difference in the 2‐year PFS between the patients that received GP PCT 2 years after undergoing GP IC and the non‐GP PCT treated patients.ConclusionsGP is not recommended for patients that have received GP IC within 2 years. Two years after undergoing GP IC, GP can be considered.
Maternity continuum of care and its determinants among mothers who gave birth in Legambo district, South Wollo, northeast Ethiopia
Authors: Cherie Niguss , Abdulkerim Mohammed , Abegaz Zinet , Walle Baze Getaw 
Published: November 2021

AbstractBackgroundMaternity continuum of care is the continuity of maternity health care services that a woman uses for antenatal care, skill birth attendant, and postnatal care. Maternal and child mortality is still big challenge in Ethiopia. Little is known about continuum of maternity care in Ethiopia and where the study area in the district revealed that there is a big discrepancy in the completion of maternity care.ObjectiveAssessment of maternity continuum of care and associated factors among mothers who gave birth in Legambo district, South, Wollo, and northeast Ethiopia.MethodA community‐based cross‐sectional study design was conducted among 732 mothers from Feb‐Mar 2020. Multistage sampling was used and data were collected through face‐to‐face interviewer‐administered semi‐structured questionnaire. Completed data were entered using Epi‐Data version 3.1, cleaned, and analyzed using SPSS version 25 Statistical Software. Descriptive statistics using Frequency, proportion, summary measures were done. Binary logistic regressions were and model fitness was checked by Hosmer and Lemeshow test which was not significant. Multivariable logistic regression was conducted and P value less than .05 and adjusted odds ratio with 95% confidence interval was considered as statistically significant.ResultThe prevalence of maternity continuum of care among mother was found 11.2% (95%, CI: 9.0‐13.8). Residence (AOR:1.837, CI:1.026‐3.288), planned pregnancy (AOR: 2.448, CI:1.361‐4.403), prepregnancy contraceptive utilization (AOR: 2.721, CI:1.469‐5.042), follow mass media (AOR: 2.33, CI:1.146‐4.736) and mother health care decision making autonomy (AOR: 3.712, CI:1.924‐7.161) were determinant factors to continuum of maternity care.ConclusionThe prevalence of maternity continuum of care in the district was low. Information education and counseling about continuum of care are still crucial. Awareness creation for both clients and care provider will improve the service. Efforts on improving and cultivating those significant factors should be done by stakeholders.
A comparative study of extensive machine learning models for predicting long‐term monthly rainfall with an ensemble of climatic and meteorological predictors
Authors: Zhou Zhengzheng , Ren Jie , He Xiaogang , Liu Shuguang 
Published: October 2021

AbstractRainfall prediction is of vital importance in water resources management. Accurate long‐term rainfall prediction remains an open and challenging problem. Machine learning techniques, as an increasingly popular approach, provide an attractive alternative to traditional methods. The main objective of this study was to improve the prediction accuracy of machine learning‐based methods for monthly rainfall, and to improve the understanding of the role of large‐scale climatic variables and local meteorological variables in rainfall prediction. One regression model autoregressive integrated moving average model (ARIMA) and five state‐of‐the‐art machine learning algorithms, including artificial neural networks, support vector machine, random forest (RF), gradient boosting regression, and dual‐stage attention‐based recurrent neural network, were implemented for monthly rainfall prediction over 25 stations in the East China region. The results showed that the ML models outperformed ARIMA model, and RF relatively outperformed other models. Local meteorological variables, humidity, and sunshine duration, were the most important predictors in improving prediction accuracy. 4‐month lagged Western North Pacific Monsoon had higher importance than other large‐scale climatic variables. The overall output of rainfall prediction was scalable and could be readily generalized to other regions.
Pain management‐related assessment and communication across the care continuum: Consensus of the opioid task force of the island peer review organization pain management coalition
Authors: Juba Katherine M. , Triller Darren , Myrka Anne , Cleary Jacqueline H. , Winans Amanda , Wahler Robert G. , Argoff Charles , Meek Patrick D. 
Published: October 2021

AbstractPrescription opioid analgesics contribute to serious, preventable adverse drug events (ADEs) and despite efforts ranging from regulatory changes to quality improvement initiatives, national studies indicate that opioid‐related emergency department visits and hospitalizations are increasing amid the coronavirus disease 2019 (COVID‐19) pandemic. Effective pain management is challenging during care transitions, and system imperfections make patients vulnerable to ADEs as they leave one care setting and move to another. Therefore, the Island Peer Review Organization (IPRO) pain management coalition convened a task force to develop resources to guide improvements in opioid‐related communication during patient care transitions. Create a consensus list of requisite communication elements (RCEs) that should accompany all patients prescribed opioids as they undergo care transitions. An interprofessional task force of pain management experts was convened and completed a blinded, iterative Delphi process. After four Delphi rounds, the panel reached consensus on a pain management discharge communication (PMDC) List of 24 RCEs across the domains of opioid dosing and pain management (nine elements), factors associated with adverse drug events (nine elements), and patient education and caregiver support (six elements). During transitions of care providers need detailed information on opioid dosing, pain characteristics, risk factors for adverse events, and patient/caregiver capacity to adequately manage symptoms while avoiding potentially fatal ADEs. The PMDC list may guide the development of new clinical tools, quality measures, accreditation standards, and checklists for pharmacists involved in the delivery of opioid stewardship during a care transition. Additional work is needed to design comprehensive, interprofessional opioid stewardship programs to ensure that the requisite communication elements are consistently available at the time of a care transition.