bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 Convalescent Sera Binding and Neutralizing Antibody Concentrations Compared with COVID-19 Vaccine Efficacy Estimates Against Symptomatic Infection
Authors: Schuh Amy J. , Satheshkumar Panayampalli S. , Dietz Stephanie , Bull-Otterson Lara , Charles Myrna , Edens Chris , Jones Jefferson M. , Bajema Kristina L. , Clarke Kristie E.N. , McDonald L. Clifford , Patel Sadhna , Cuffe Kendra , Thornburg Natalie J. , Schiffer Jarad , Chun Kelly , Bastidas Monique , Fernando Manory , Petropoulos Christos J. , Wrin Terri , Cai Suqin , Adcock Dot , Sesok-Pizzini Deborah , Letovsky Stanley , Fry Alicia M. , Hall Aron J. , Gundlapalli Adi V. 
Published: November 2021

ABSTRACTPrevious vaccine efficacy (VE) studies have estimated neutralizing and binding antibody concentrations that correlate with protection from symptomatic infection; how these estimates compare to those generated in response to SARS-CoV-2 infection is unclear. Here, we assessed quantitative neutralizing and binding antibody concentrations using standardized SARS-CoV-2 assays on 3,067 serum specimens collected during July 27, 2020-August 27, 2020 from COVID-19 unvaccinated persons with detectable anti-SARS-CoV-2 antibodies using qualitative antibody assays. Quantitative neutralizing and binding antibody concentrations were strongly positively correlated (r=0.76, p<0.0001) and were noted to be several fold lower in the unvaccinated study population as compared to published data on concentrations noted 28 days post-vaccination. In this convenience sample, ∼88% of neutralizing and ∼63-86% of binding antibody concentrations met or exceeded concentrations associated with 70% COVID-19 VE against symptomatic infection from published VE studies; ∼30% of neutralizing and 1-14% of binding antibody concentrations met or exceeded concentrations associated with 90% COVID-19 VE. These data support observations of infection-induced immunity and current recommendations for vaccination post infection to maximize protection against symptomatic COVID-19.
Large-scale seroepidemiology uncovers nephrological pathologies in people with tau autoimmunity
Authors: Magalhães Andreia D. , Emmenegger Marc , De Cecco Elena , Carta Manfredi , Frontzek Karl , Chincisan Andra , Guo Jingjing , Hornemann Simone , Aguzzi Adriano 
Published: November 2021

AbstractImportanceIntraneuronal aggregates of the microtubule-associated protein tau play a pivotal role in Alzheimer’s disease and several other neurodegenerative syndromes. Anti-tau antibodies can reduce pathology in mouse models of neurodegeneration and are currently being tested in humans.ObjectiveTo evaluate the seroprevalence of natural anti-tau autoantibodies and their potential association with diseases.DesignIn a cross-sectional study, plasma samples from a randomized university hospital cohort and healthy blood donors, collected from 2017 to 2020, were screened for anti-MTBD-tau plasma IgG autoantibodies (ατ) in a miniaturized, high-throughput ELISA. Samples were included if basic demographic data was available and age was ≥18 years. Data analysis was performed from De-cember 2017 to August 2024. Titers were defined as −log10(EC50) values. Titers of ≥1.8 were clas-sified as ατ+.ParticipantsBlood was obtained during routine clinical care from 32,291 patients admitted as inpatients or outpatients to any department of a university hospital, or from 8,206 healthy blood donors.ResultsHigh-titer ατ+samples were surprisingly prevalent, with hospital patients being three times more likely to be ατ+than healthy blood donors (4.8% vs 1.6%). ατ autoantibodies ligated selectively tau, inhibited tau aggregation in vitro, and interfered with tau detection in plasma sam-ples. No association was found between ατ autoantibodies and neurologic disorders. Instead, tau autoreactivity showed a significant association with kidney and urinary disorders (adjusted risk ratio (RR) 1.27, 95% CI 1.10-1.45, P=0.001 and 1.40, 95% CI 1.20-1.63, P<0.001 respectively).Conclusions and RelevanceWhile ατ autoimmunity was not associated with neurologic dis-eases, its association with extraneural pathologies will require scrutiny in the context of anti-tau therapies. Moreover, immunochemical plasma tau assays were confounded by ατ antibodies, whose high prevalence may limit their usefulness as biomarker.
Examining the intersection of child protection and public housing: development, health and justice outcomes using linked administrative data
Authors: Malvaso Catia , Montgomerie Alicia , Pilkington Rhiannon , Baker Emma , Lynch John 
Published: November 2021

AbstractObjectiveWe described development, health and justice system outcomes for children in contact with child protection and public housing.DesignDescriptive analysis of outcomes for children known to child protection who also had contact with public housing drawn from the South Australian (SA) Better Evidence Better Outcomes Linked Data (BEBOLD) platform.SettingThe BEBOLD platform holds linked administrative records collected by government agencies for whole-population successive birth cohorts in South Australia beginning in 1999.ParticipantsThis study included data from birth registrations, perinatal, child protection, public housing, hospital, emergency department, early education, and youth justice for all SA children born 1999-2013 and followed until 2016. The base population notified at least once to child protection was n=67,454.Primary outcome measureContact with the public housing system.Secondary outcome measureshospitalisations and emergency department presentations before age 5, and early education at age 5, and youth justice contact before age 17.ResultsMore than 60% of children with at least one notification to child protection had contact with public housing, and 60.2% of those known to both systems were known to housing first. Children known to both systems experienced more emergency department and hospitalisation contacts, greater developmental vulnerability and were about 6 times more likely to have youth justice system contact.ConclusionsThere is substantial overlap between involvement with child protection and public housing in SA. Those children are more likely to face a life trajectory characterised by greater contact with the health system, greater early life developmental vulnerability, and greater contact with the criminal justice system. Ensuring the highest quality of supportive early life infrastructure for families in public housing may contribute to prevention of contact with child protection and better life trajectories for children.Strengths and limitations of this studyThis descriptive study provides epidemiological insight into the largely unexplored intersection between child protection and housing systems using whole-of-population linked administrative dataThe findings of this study are based on data drawn from South Australia and may not be directly generalizable to other locations because of different administrative systemsDespite data being drawn from one Australian jurisdiction, the qualitative relationship between child protection and housing is likely to be similar
Cryo-EM structure of hexameric yeast Lon (PIM1) highlights importance of conserved structural elements
Authors: Yang Jie , Song Albert S. , Wiseman R. Luke , Lander Gabriel C. 
Published: November 2021

AbstractLon protease is a conserved ATP-dependent serine protease composed of an AAA+ domain that mechanically unfolds substrates and a serine protease domain that degrades unfolded substrates. In yeast, dysregulation of Lon protease (PIM1) attenuates lifespan and leads to gross mitochondrial morphologic perturbations. Although structures of bacterial and human Lon protease reveal a hexameric assembly, PIM1 was speculated to form a heptameric assembly, and is uniquely characterized by a ~50 residue insertion between the ATPase and protease domains. To understand the yeast-specific properties of PIM1, we determined a high-resolution cryo-EM structure of PIM1 in a substrate-translocating state. Here, we reveal that PIM1 forms a hexamer, conserved with that of bacterial and human Lon proteases, wherein the ATPase domains form a canonical closed spiral that enables pore loop residues to translocate substrate to the protease chamber. In the substrate-translocating state, PIM1 protease domains form a planar protease chamber in an active conformation and are uniquely characterized by a ~15 residue C-terminal extension. These additional C-terminal residues form an alpha-helix that is located along the base of the protease domain. Finally, we did not observe density for the yeast-specific insertion between the ATPase and protease domains, likely due to high conformational flexibility. Biochemical studies to investigate the insertion using constructs that truncated or replaced the insertion with a glycine-serine linker suggest that the yeast-specific insertion is dispensable for PIM1’s enzymatic function. Altogether, our structural and biochemical studies highlight unique components of PIM1 machinery and demonstrate evolutionary conservation of Lon protease function.
The DOMINO web-server for active module identification analysis
Authors: Levi Hagai , Rahmanian Nima , Elkon Ran , Shamir Ron 
Published: November 2021

AbstractActive module identification (AMI) is an essential step in many omics analyses. Such algorithms receive a gene network and a gene activity profile as input and report subnetworks that show significant over-representation of accrued activity signal (“active modules”). Such modules can point out key molecular processes in the analyzed biological conditions.ResultsWe recently introduced a novel AMI algorithm called DOMINO, and demonstrated that it detects active modules that capture biological signals with markedly improved rate of empirical validation. Here, we provide an online server that executes DOMINO, making it more accessible and user-friendly. To help the interpretation of solutions, the server provides GO enrichment analysis, module visualizations, and accessible output formats for customized downstream analysis. It also enables running DOMINO with various gene identifiers of different organisms.Availability and implementationThe server is available athttp://domino.cs.tau.ac.il. Its codebase is available athttps://github.com/Shamir-Lab.
Possible Regulation of Toll-Like Receptor 4 By Lysine Acetylation Through LPCAT2 Activity in RAW264.7 Cells
Authors: Poloamina Victory Ibigo , Abate Wondwossen , Fejer Gyorgy , Jackson Simon K. 
Published: November 2021

1AbstractInflammation is central to several diseases. TLR4 mediates inflammatory signals, however, there are gaps in the understanding of its mechanisms. Recently, TLR4 was found to co-localise with LPCAT2, a lysophospholipid acetyltransferase. This interaction influenced TLR4 subcellular localisation through an unknown mechanism.In this study, we have combined computational analysis, RNA interference technology, and biochemical analysis to investigate the possibility of TLR4 lysine acetylation and the influence of LPCAT2 on the detected lysine acetylation.The results suggest for the first time that TLR4 can undergo lysine acetylation and LPCAT2 can influence TLR4 lysine acetylation. This lays a foundation for further research on the role of lysine acetylation on TLR4 and characterisation of LPCAT2 as a protein acetyltransferase.
Cryo-EM structure of the Smc5/6 holo-complex
Authors: Hallett Stephen T. , Harry Isabella Campbell , Schellenberger Pascale , Zhou Lihong , Cronin Nora B. , Baxter Jonathan , Etheridge Thomas J. , Murray Johanne M. , Oliver Antony W. 
Published: November 2021

ABSTRACTThe Smc5/6 complex plays an essential role in the resolution of recombination intermediates formed during mitosis or meiosis, or as a result of the cellular response to replication stress. It also functions as a restriction factor preventing viral integration. Here, we report the cryo-EM structure of the six-subunit budding yeast Smc5/6 holo-complex, reconstituted from recombinant proteins expressed in insect cells – providing a full overview of the complex in its apo / non-liganded form, and revealing how the Nse1/3/4 subcomplex binds to the hetero-dimeric SMC protein core. In addition, we demonstrate that a region within the head domain of Smc5, equivalent to the ‘W-loop’ of Smc4 or ‘F-loop’ of Smc1, mediates an essential interaction with Nse1. Taken together, these data confirm a degree of functional equivalence between the structurally unrelated KITE and HAWK accessory subunits associated with SMC complexes.
Lipid droplets control mitogenic lipid mediator production in human cancer cells
Authors: Jarc Jovičić Eva , Pucer Janež Anja , Eichmann Thomas O. , Koren Špela , Brglez Vesna , Jordan Paul M. , Gerstmeier Jana , Lainšček Duško , Golob-Urbanc Anja , Jerala Roman , Lambeau Gérard , Werz Oliver , Zimmermann Robert , Petan Toni 
Published: November 2021

AbstractPolyunsaturated fatty acids (PUFAs) are components of membrane phospholipids and precursors of bioactive lipid mediators. Here, we investigated the crosstalk of three pathways providing PUFAs for lipid mediator production: (i) secreted group X phospholipase A2(GX sPLA2) and (ii) cytosolic group IVA PLA2(cPLA2α), which both mobilize PUFAs from phospholipids, and (iii) adipose triglyceride lipase (ATGL), which breaks down triacylglycerols (TAGs) stored in lipid droplets (LDs). Combining lipidomic and functional analyses, we demonstrate that lipid mediator production depends on TAG turnover. GX sPLA2directs PUFAs into TAGs and ATGL is required for their entry into lipid mediator biosynthetic pathways. ATGL also promotes the incorporation of LD-derived PUFAs into phospholipids representing substrates for cPLA2α. Additionally, inhibition of TAG synthesis mediated by acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) reduces the levels of mitogenic lipid signals and compromises tumour growth. This study expands the paradigm of PLA2-driven lipid mediator signalling and identifies LDs as central lipid mediator production hubs.
In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity
Authors: Marashi Hasan , Beihaghi Maria , Khaksar Samad 
Published: November 2021

AbstractCCL21 has an essential role in anti-tumor immune activity. Epitopes of IL1β have adjuvant activity without causing inflammatory responses. CCR7 and its ligands play a vital role in the immune balance; specifically, in transport of T lymphocytes and antigen-presenting cells such as dendritic cells to the lymph nodes. This study aimed to produce epitopes of CCL21 and IL1β as a recombinant protein and characterize it’s in vitro anti-tumor and immunogenic activity. A codon-optimized ccl21/IL1β gene was designed and synthesized from human genes. Stability and binding affinity of CCL21/IL1β protein and CCR7 receptor were examined through in silico analyses. The construct was introduced into N. tabacum to produce this recombinant protein and the structure and function of CCL21/IL1β were examined. Purified protein from transgenic leaves generated a strong signal in SDS PAGE and western blotting assays. FTIR measurement and MALDI-TOF/TOF mass spectrography showed that ccl21/IL-1β was correctly expressed in tobacco plants. Potential activity of purified CCL21/IL1β in stimulating the proliferation and migration of MCF7CCR7+ cancer cell line was investigated using the wound healing method. The results demonstrated a decrease in survival rate and metastasization of cancer cells in the presence of CCL21/IL1β, and IC50 of CCL21 on MCF7 cells was less than that of non-recombinant protein. Agarose assay on PBMCsCCR7+ showed that CCL21/IL1β has biological activity and there is a distinguishable difference between chemokinetic (CCL21) and chemotactic (FBS) movements. Overall, the results suggest that CCL21/IL1β could be considered an effective adjuvant in future in vivo and clinical tests.
Development of a novel glycoengineering platform for the rapid production of conjugate vaccines
Authors: Abouelhadid Sherif , Atkins Elizabeth , Kay Emily , Passmore Ian , North Simon J , Lehri Burhan , Hitchen Paul , Bakke Eirik , Rahman Mohammed , Bosse Janine , Li Yanwen , Terra Vanessa S. , Langford Paul , Dell Anne , Wren Brendan W , Cuccui Jon 
Published: November 2021

AbstractAntimicrobial resistance (AMR) is threatening the lives of millions worldwide. Antibiotics which once saved countless lives, are now failing, ushering in vaccines development as a current global imperative. Conjugate vaccines produced either by chemical synthesis or biologically in Escherichia coli cells, have been demonstrated to be safe and efficacious in protection against several deadly bacterial diseases. However, conjugate vaccines assembly and production have several shortcomings which hinders their wider availability. Here, we developed a tool, Mobile-element Assisted Glycoconjugation by Insertion on Chromosome, MAGIC, a novel method that overcomes the limitations of the current conjugate vaccine design method(s). We demonstrate at least 2-fold increase in glycoconjugate yield via MAGIC when compared to conventional bioconjugate method(s). Furthermore, the modularity of the MAGIC platform also allowed us to perform glycoengineering in genetically intractable bacterial species other than E. coli. The MAGIC system promises a rapid, robust and versatile method to develop vaccines against bacteria, especially AMR pathogens, and could be applied for biopreparedness.
Designing and implementing performance measurement systems based on enterprise engineering guidelines
Authors: Moura Louisi Francis , Pinheiro de Lima Edson , Deschamps Fernando , Etzion Dror , Gouvea da Costa Sergio E. 
Published: November 2021

PurposeThis conceptual paper presents a proposal for improving a performance measurement (PM) system implementation process based on enterprise engineering (EE) guidelines, which gives the process a sense of completeness.Design/methodology/approachThis paper analyzes a well-known process for PM systems implementation organized in two phases: identifying, designing and implementing the top-level performance measures; and cascading the top-level measures and identify appropriate lower-level performance measures. The proposed improvements to the studied process derive from the EE guidelines, which establish a basis for the structure of an organizational management system, the formalization and synchronization of processes, performance expectations, exception handling and change management.FindingsThe study reveals that not all EE guidelines are covered by the analyzed process, with four of them having no evidence of being adopted: involvement of people in process design and implementation; ensuring interoperability between different systems in the information structure; addressing of all possible exceptions; coherence and consistency of semantics across all processes.Originality/valueBy the lens of EE guidelines, this paper advances a how-to-guide. This paper can support managers and researchers on PM system design and implementation, given the importance and relevance of EE recommendations having a consistent and well-structured procedure.
WOW, the make-up AR app is impressive: a comparative study between China and South Korea
Authors: Butt Asad , Ahmad Hassan , Muzaffar Asif , Ali Fayaz , Shafique Nouman 
Published: November 2021

Purpose
Consumers today actively participate in online purchasing experiences. As a result, it is critical to comprehend the behavioral aspects of novel technology usage, such as augmented reality (AR). AR apps enable beauty companies to create and design more immersive experience services. This study aims to highlight consumers’ perspectives on their continued desire to use AR app services.

Design/methodology/approach
A comparative study between China and South Korea was conducted with sample sizes of 458 and 315, respectively. Smart PLS was used for analysis.

Findings
The findings suggest that AR apps influence innovative consumers in China and South Korea to be satisfied with and continue to use such services. Previous research on technology acceptance model, information system success, AR and artificial intelligence (AI)-context-specific variables supported the findings.

Practical implications
This study contributes to the development of AR apps for beauty brands, as such technology revolutionizes how beauty brands work and grow. As a result, AR apps can pave the way for brands to provide an immersive experience to their customers.

Originality/value
The current study contributes to AR and AI drivers in the context of beauty brands by using novel technologies such as AR. AR integration with AI-context-specific variables indicates that consumers in China and South Korea are innovative and accept such technologies when purchasing beauty products online.
Sustainability performance and social media: an explorative analysis
Authors: Russo Sarah , Schimperna Federico , Lombardi Rosa , Ruggiero Pasquale 
Published: November 2021

Purpose
This paper aims to present a deep understanding of how social media affects organisations’ sustainability performance, using environmental, social and governance (ESG) factors. Particularly, this paper assumes the existence of a causal relationship between organisations’ sustainability performance and the use of their social media profile (i.e. Twitter).

Design/methodology/approach
The authors used a multivariate regression with an explorative approach. Using Thomson Reuters Eikon, the authors composed a sample of 115 public EU companies with a headquarter in Europe operating in the “energy” and “utilities” sectors. The authors collected ESG-related, financial and Twitter-related data covering the period 2016–2019.

Findings
The study findings emphasise the existence of a statistically significant and positive relationship between social media profiles (i.e. Twitter) and companies’ sustainability performance. Findings show that ESG-oriented companies use their Twitter profile more as a tool for achieving a higher level of legitimation rather than for managing their sustainability strategy and related performance. Therefore, social media contribute more to the construction of companies’ CSR identity than the management of analytic aspects of sustainability performance. The longevity of companies’ profiles is the variable mostly showing a causal relationship not only with the general measure of companies’ sustainability performance but also with its pillars and sub-pillars.

Originality/value
This research is original in showing academics, practitioners and policymakers results on the impact of different modalities of interaction (retweets, replies, likes and quotes) between organisations and stakeholders by using social media on sustainability performance.