bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Pathogenic Pseudomonas alcaligenes Isolated from Koi Carp in China
Authors: Bai Jie , Huo Yian , Hu Xiucai , Lü Aijun , Sun Jingfeng 
Published: July 2021

Abstract
Pseudomonas alcaligenes infection is rare in aquaculture. In this study, we provide the first report on the characterization of P. alcaligenes from koi (a variant of Common Carp Cyprinus carpio) in China. A gram-negative bacterium was isolated from the diseased koi and was named KCP-516. Morphological and biochemical tests as well as phylogenetic tree analyses derived from 16S ribosomal RNA, gyrase subunit A, and gyrase subunit B gene sequencing all strongly indicated that the isolate KCP-516 was P. alcaligenes. In liquid medium, the optimal growth conditions were 25°C, 2.5% NaCl, and pH 8. The pathogenicity of the isolate was demonstrated in koi, with 7.0 × 104 CFU/g fish weight identified as the dose lethal to 50% of test fish. The results will provide a scientific reference for the diagnosis and treatment of P. alcaligenes infection.
Discovery and Characterization of Spike N‐Terminal Domain‐Binding Aptamers for Rapid SARS‐CoV‐2 Detection
Authors: Kacherovsky Nataly , Yang Lucy F. , Dang Ha V. , Cheng Emmeline L. , Cardle Ian I. , Walls Alexandra C. , McCallum Matthew , Sellers Drew L. , DiMaio Frank , Salipante Stephen J. , Corti Davide , Veesler David , Pun Suzie H. 
Published: July 2021

AbstractThe coronavirus disease 2019 (COVID‐19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS‐CoV‐2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo‐EM, we demonstrate that SNAP1 (SARS‐CoV‐2 spike protein N‐terminal domain‐binding aptamer 1) binds to the S N‐terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV‐inactivated SARS‐CoV‐2 at concentrations as low as 5×105 copies mL−1. SNAP1 is therefore a promising molecular tool for SARS‐CoV‐2 diagnostics.
Adaptive type‐II fuzzy nonsingular fast terminal sliding mode controller using fractional‐order manifold for second‐order chaotic systems
Authors: Labbaf Khaniki Mohammad Ali , Tavakoli‐Kakhki Mahsan 
Published: July 2021

AbstractA novel fuzzy adaptive finite‐time controller is designed for synchronization and control of the Duffing–Holmes and the gyroscope systems in the presence of uncertainty and external disturbance. The fractional‐order fast nonsingular terminal sliding mode manifold is used to ensure the finite‐time control, speed up the convergence rate, and address the singularity problem of the conventional terminal sliding mode controller. Also, the novel control law successfully reduces the chattering phenomenon. Adaptive biased type‐II fuzzy inference system is used to determine the optimal values of the switching control term. The adaptation rules of the novel fuzzy inference system are achieved based on Lyapunov stability theorem. Finally, by using numerical simulations, the effectiveness and superiority of the proposed control scheme are verified against some other methods.
Qualitatively and quantitatively investigating the metabolism of 20(S)‐protopanaxadiol‐type ginsenosides by gut microbiota of different species
Authors: Zhang Ying , Yao Lingling , Tang Chunping , Jiang Jianlan , Ye Yang , Liu Jia 
Published: July 2021

AbstractGinsenosides Rb1, Rb2, Rb3 and Rc, four major protopanaxadiol (PPD)‐type ginsenosides, can be metabolized by gut microbiota. The composition of gut microbiota varies in different species. Existing publications have reported the metabolite fates of ginsenosides by gut microbiota from single species. However, their microbiota‐related metabolic species differences have not been evaluated yet. In current study, in vitro anaerobic incubations of PPD‐type ginsenosides with gut microbiota from humans, rabbits and rats were conducted. The metabolites of each ginsenoside were then identified by LC–MS. A total of 15 metabolites from the four ginsenosides were identified. The major metabolic pathways were stepwise removals of the C‐20 and C‐3 sugar moieties to obtain aglycone PPD. The results showed that the hydrolysis rate of C‐20 terminal β‐D‐glucopyranosyl was significantly higher than those of α‐L‐arabinopyranosyl, β‐D‐xylopyranosyl and α‐L‐arabinofuranosyl in different species. The activity of β‐glucosidase, the metabolic rates of parent compounds and the formation rates of their metabolites were significantly higher in gut microbiota from rabbits than from humans and rats. Our research draws researchers’ attention to the species differences of microbiota‐related drug metabolism.
Neglected but not negligible aspects of antidepressants and their availability in bipolar depression
Authors: Terao Takeshi 
Published: July 2021

AbstractObjectivesAlthough many antidepressants are available, they are not always used appropriately. For appropriate use of antidepressants, the old concept of a linear dose–response relationship, in which the dose is linearly increased to achieve a sufficient antidepressant effect, should be reconsidered. Furthermore, there is ongoing debate on the safe and appropriate use of antidepressants in patients with bipolar depression. Antidepressants may be used under certain conditions in patients with bipolar depression. These neglected—but not negligible—aspects of antidepressants have been discussed herein.MethodsA narrative qualitative reviewResultsDose–response relationships of antidepressants such as selective serotonin reuptake inhibitors (SSRIs) are not linear. They may be bell‐shaped, with efficacy initially increasing with an increase in dose but decreasing when the dose is increased beyond a certain point. Despite using international diagnostic criteria, uncertainty remains on whether operationally diagnosed depression is latent bipolar I depression, latent bipolar II depression, or true depression. Furthermore, operationally diagnosed bipolar II depression may be latent bipolar I depression, true bipolar II depression, or depression with false hypomanic episodes. Manic/hypomanic switches are most likely to occur in patients receiving tricyclic antidepressants, followed by those receiving serotonin and noradrenaline reuptake inhibitors and SSRIs, in that order. Also, these switches are most likely to occur in patients with bipolar I depression, followed by those with bipolar II depression and true depression, in that order.ConclusionsConsidering the diagnostic subtype of bipolar depression and antidepressant properties may help to determine the optimal treatment strategy.
Towards a holistic framework for sustainable value analysis in business models: A tool for sustainable development
Authors: Méndez‐León Eduardo , Reyes‐Carrillo Tatiana , Díaz‐Pichardo René 
Published: July 2021

AbstractThis article analyzes how the concept of sustainable value is used in the business model literature to understand and structure the concepts used in analyzing the value creation process in business models to date. We found that in discussing sustainable business models, sustainable value, a term encompassing the environmental, social, and economic benefits of a given model, has been used in place of the conventional term, value. However, the conceptual implications of this substitution have not been sufficiently addressed. A systematic literature review reveals both positive and negative perspectives of value as well as the misuse of several multilevel value concepts. We categorize these concepts according to their meaning, differences, similarities, and implications. As a result, we propose a holistic framework that structures them, integrates both positive and negative perspectives of value, and proposes an activity‐based analysis for sustainable value in business models. The contributions of this paper are twofold: theoretically, it structures value concepts in order to better understand the sustainable value process in business models, and practically, it offers a tool for sustainable development by providing a framework to analyze sustainable value in a variety of business models as a basis for improvement in sustainability.
Inflammatory breast cancer appearance at presentation is associated with overall survival
Authors: Balema Wintana , Liu Diane , Shen Yu , El‐Zein Randa , Debeb Bisrat G. , Kai Megumi , Overmoyer Beth , Miller Kathy D. , Le‐Petross Huong T. , Ueno Naoto T. , Woodward Wendy A. 
Published: July 2021

AbstractBackgroundInflammatory breast cancer (IBC) is a clinical diagnosis. Here, we examined the association of a “classic” triad of clinical signs, swollen involved breast, nipple change, and diffuse skin change, with overall survival (OS).MethodBreast medical photographs from patients enrolled on a prospective IBC registry were scored by two independent reviewers as classic (triad above), not classic, and difficult to assign. Chi‐squared test, Fisher's exact test, and Wilcoxon rank‐sum test were used to assess differences between patient groups. Kaplan–Meier estimates and the log‐rank test and Cox proportional hazard regression were used to assess the OS.ResultsWe analyzed 245 IBC patients with median age 54 (range 26–81), M0 versus M1 status (157 and 88 patients, respectively). The classic triad was significantly associated with smoking, post‐menopausal status, and metastatic disease at presentation (p = 0.002, 0.013, and 0.035, respectively). Ten‐year actuarial OS for not classic and difficult to assign were not significantly different and were grouped for further analyses. Ten‐year OS was 29.7% among patients with the classic sign triad versus 57.2% for non‐classic (p < 0.0001). The multivariate Cox regression model adjusting for clinical staging (p < 0.0001) and TNBC status (<0.0001) demonstrated classic presentation score significantly associated with poorer OS time (HR 2.6, 95% CI 1.7–3.9, p < 0.0001).ConclusionsA triad of classic IBC signs independently predicted OS in patients diagnosed with IBC. Further work is warranted to understand the biology related to clinical signs and further extend the understanding of physical examination findings in IBC.
Male professional footballers’ use of mental skills training and counselling: Dilemma or conundrum?
Authors: Abela Daniel , Falzon Ruth , Muscat Adele 
Published: July 2021

AbstractBackgroundThe study explores male footballers’ experiences of Mental Skills Training (MST) – usually carried out by sports psychologists, and counselling (Herzog & Hays, 2012).MethodThis research's aim begged a qualitative approach – interpretative phenomenological analysis. Semi‐structured, audio‐recorded interviews were used with seven participant‐footballers.FindingsParticipants faced a number of unique challenges throughout their career and consider support pivotal for their success in the industry. Parental and institutional support were key sources for support. Participants experienced counselling as a safe and reliable space, where the therapeutic relationship extended beyond football‐related issues. Participants explained that MST can be self‐taught, is a direct hands‐on approach, can be practised whilst injured, and a first point of contact with other services and forms of therapeutic support. Views on their expectations of MST and counselling services varied: either compatible and should be delivered by the same person, or different and should delivered separately. Participants identified others’ perceptions as possible challenges for footballers accessing MST and counselling.RecommendationsParticipants recommended further research with other athletes, with footballers from diverse countries and cultures, with other helping professionals, with leaders, with managers and sponsors of football, and with sports in general. Participants suggested counsellor‐training in sport psychology, sports psychologists and counsellors’ participation in sporting contexts, and developing working relationships with kindred professions. They also argued for awareness campaigns with the general public and with stakeholders. An amendment to the local national policy should incorporate mental and social well‐being of athletes as a priority for sporting institutions.
PROTOCOL: Effectiveness of interventions for improving social inclusion outcomes for people with disabilities in low‐ and middle‐income countries: A systematic review
Authors: Saran Ashrita , Hunt Xanthe , White Howard , Kuper Hannah 
Published: July 2021

AbstractThe objectives of this review are to: (1) examine the effectiveness of interventions for improving social inclusion outcomes for people with disabilities (physical, visual, hearing, intellectual or mental health conditions) in low‐ and middle‐income countries (LMICs); and (2) to critically appraise the confidence in study finding of the included studies. Key questions include: (1) Are interventions to improve social inclusion outcomes for people with disabilities in LMICs effective, and what is the quality of evidence base? (2) What types of intervention, or intervention design features, are most effective in improving social inclusion outcomes for people with disabilities in LMICs? (3) Which interventions appear most effective for different categories of disability? (4) What are the barriers to people with disabilities participating in interventions to improve their social inclusion outcomes? And what factors facilitate participation in, and the success of, such interventions?
Meta‐analysis of cryoballoon ablation versus antiarrhythmic drugs as initial therapy for symptomatic atrial fibrillation
Authors: Mao Yin‐jun , Feng Wei‐ye , Huang Qun‐ying , Yu Fu‐ling , Chen Jian‐xing , Wang Hang 
Published: July 2021

AbstractBackgroundThe optimal first‐line approach for patients with symptomatic atrial fibrillation (AF) remains unclear. We compared the efficacy and safety of cryoballoon ablation (CBA) and antiarrhythmic drugs (AADs) in the initial management of symptomatic AF.HypothesisCBA is superior to AAD as initial therapy for symptomatic AF.MethodsWe searched the EMBASE, PubMed, and Cochrane Library databases for randomized controlled trials (RCTs) that compared CBA with AAD as first‐line treatment for AF from the date of database establishment until March 18, 2021. The risk ratio (RR) with a 95% confidence interval (CI) was used as a measure of treatment effect.ResultsThree RCTs that enrolled 724 patients in total were included in this meta‐analysis. Majority of the patients were relatively young and had paroxysmal AF. CBA was associated with a significant reduction in the recurrence of atrial arrhythmia compared with AAD therapy, with low heterogeneity (RR, 0.59; 95% CI, 0.49–0.71; p < .00001; I2 = 0%). There was a significant difference in the rate of symptomatic atrial arrhythmia recurrence (RR, 0.44; 95% CI, 0.29–0.65; p < .0001; I2 = 0%); however, the rate of serious adverse events was similar between the two treatment groups (RR: 1.18; 95% CI: 0.71–1.97, p = .53; I2 = 0%). Transient phrenic nerve palsy occurred in four patients after the CBA procedure.ConclusionThe current meta‐analysis suggests that CBA is more effective than AAD as initial therapy in patients with symptomatic paroxysmal AF. Serious iatrogenic adverse events are uncommon in CBAs.
Characteristic analysis and suppression of SiC MOSFET‐based crosstalk for inductive power transfer systems
Authors: Bo Qiang , Zhang Yuwang , Guo Yanjie , Wang Lifang 
Published: July 2021

AbstractDue to the lower gate threshold voltage of silicon carbide (SiC) MOSFET as compared with the silicon (Si) MOSFET, crosstalk significantly limits the high‐speed switching performance of SiC MOSFET‐based inductive power transfer (IPT) systems. The paper studies the crosstalk characteristic and proposes a suppression method of crosstalk aiming at IPT systems with phase‐shifted control. Firstly, the generation mechanism of crosstalk is modeled and analyzed. Then, based on the analysis results, the special crosstalk problem for IPT systems with phase‐shifted control is discussed, and a crosstalk suppression method is analyzed based on the optimization of compensation network parameters. Finally, a 1‐kW IPT system prototype is built for experimental verification. The experimental results show that the optimization method can effectively suppress the crosstalk problem of the IPT system with phase‐shifted control and does not significantly affect the output power and transmission efficiency.
Plastic adjustments of biparental care behavior across embryonic development under elevated temperature in a marine ectotherm
Authors: Spatafora Davide , Massamba N'Siala Gloria , Quattrocchi Federico , Milazzo Marco , Calosi Piero 
Published: July 2021

AbstractPhenotypic plasticity in parental care investment allows organisms to promptly respond to rapid environmental changes by potentially benefiting offspring survival and thus parental fitness. To date, a knowledge gap exists on whether plasticity in parental care behaviors can mediate responses to climate change in marine ectotherms. Here, we assessed the plasticity of parental care investment under elevated temperatures in a gonochoric marine annelid with biparental care, Ophryotrocha labronica, and investigated its role in maintaining the reproductive success of this species in a warming ocean. We measured the time individuals spent carrying out parental care activities across three phases of embryonic development, as well as the hatching success of the offspring as a proxy for reproductive success, at control (24℃) and elevated (27℃) temperature conditions. Under elevated temperature, we observed: (a) a significant decrease in total parental care activity, underpinned by a decreased in male and simultaneous parental care activity, in the late stage of embryonic development; and (b) a reduction in hatching success that was however not significantly related to changes in parental care activity levels. These findings, along with the observed unaltered somatic growth of parents and decreased brood size, suggest that potential cost‐benefit trade‐offs between offspring survival (i.e., immediate fitness) and parents' somatic condition (i.e., longer‐term fitness potential) may occur under ongoing ocean warming. Finally, our results suggest that plasticity in parental care behavior is a mechanism able to partially mitigate the negative effects of temperature‐dependent impacts.
Structural, Physical, Theoretical and Spectroscopic Investigations of Mixed‐Valent Eu2Ni8Si3 and Its Structural Anti‐Type Sr2Pt3Al8
Authors: Stegemann Frank , Block Theresa , Klenner Steffen , Zhang Yuemei , Fokwa Boniface P. T. , Doerenkamp Carsten , Eckert Hellmut , Janka Oliver 
Published: July 2021

AbstractEu2Ni8Si3 and its anti‐type representative Sr2Pt3Al8 were synthesized from the elements. They crystallize in the tetragonal crystal system with space group P42/nmc and with lattice parameters of a=997.9(1) and c=747.6(1) pm (Eu2Ni8Si3) as well as a=1082.9(2) and c=823.3(2) pm (Sr2Pt3Al8). Both compounds were investigated via single crystal X‐ray diffraction, indicating slight Si/Ni mixing for the silicide. Sr2Pt3Al8 exhibits a temperature independent magnetic susceptibility, suggesting superimposed dia‐ and Pauli‐paramagnetic contributions. The independent Al and Pt sites of the platinide were further characterized by 27Al and 195Pt solid‐state NMR spectroscopy, which were assigned with the help of electronic structure calculations. ICOHP calculations and Bader charges were used to analyze the bonding situation. Eu2Ni8Si3 in contrast is paramagnetic with a ferromagnetic transition at TC=46.9(2) K and exhibits an effective magnetic moment of μeff=6.61(1) μB per Eu atom. The latter is in line with an intermediate valence that was further proven by 151Eu Mößbauer spectroscopic investigations. At 300 K, the refined Eu2+/Eu3+ ratios are 60 %/40 %, at 78 K 62 % and 38 % (Eu2+/Eu3+) are observed, being in line with the ratio deduced from the magnetic susceptibility. Finally, at 6 K a ratio of 68 % Eu2+ and 32 % Eu3+ was observed. Below the Curie temperature, the Eu2+ signal shows a full magnetic hyperfine splitting, with an internal magnetic field value of B0=28.4 T.
Application of bio and chemical fertilizers improves yield, and essential oil quantity and quality of Moldavian balm (Dracocephalum moldavica L.) intercropped with mung bean (Vigna radiata L.)
Authors: Faridvand Shahin , Rezaei‐Chiyaneh Esmaeil , Battaglia Martin Leonardo , Gitari Harun I. , Raza Muhammad Ali , Siddique Kadambot H. M. 
Published: July 2021

AbstractIntercropping Moldavian balm with mung bean is an ecological approach for improving resource productivity. A field experiment was conducted over two growing seasons (2018 and 2019) to determine the effect of fertilizer application on yield and essential oil (EO) productivity of Moldavian balm intercropped with mung bean. The experiment had a two‐factor randomized complete block design (RCBD) with three replicates. The first factor comprised of five cropping patterns: Moldavian balm sole crop (MBs), mung bean sole crop (MGs), one row each of Moldavian balm +mung bean (1MB:1MG), two rows each of Moldavian balm +mung bean (2MB:2MG), and three rows of Moldavian balm +two rows of mung bean (3MB:2MG). The second factor comprised four fertilizer sources: no fertilizer application (C, control), 100% chemical fertilizer (NPK), 50% chemical fertilizer +100% bacterial fertilizer (NPK+BF), and 100% bacterial fertilizer +100% mycorrhizal fungi (BF+MF). The sole crop fertilized with NPK+BF produced the highest seed yields for MG (1189 kg/ha) and MB (7027 kg/ha), while 3MB:2MG fertilized with NPK+BF had the highest nutrient contents. Moldavian balm produced the highest EO content and yield in 2MB:2MG fertilized with NPK+BF. The EO of MB mainly comprised geranyl acetate (30–39%), geranial (20–31%), neral (18–24%), and geraniol (3–8%). In addition, the 3MB:2MG intercropping treatment fertilized with NPK+BF had the highest land equivalent ratio (LER = 1.35). We recommend an intercropping ratio of 2MB:2MG fertilized with NPK+BF is recommended as an alternative and eco‐friendly strategy for farmers to improve EO quantity and quality.
Morphofunctional programming of microglia requires distinct roles of type II myosins
Authors: Melo Pedro Neves , Souza da Silveira Mariana , Mendes Pinto Inês , Relvas João Bettencourt 
Published: July 2021

AbstractThe ramified morphology of microglia and the dynamics of their membrane protrusions are essential for their functions in central nervous system development, homeostasis, and disease. Although their ability to change and control shape critically depends on the actin and actomyosin cytoskeleton, the underlying regulatory mechanisms remain largely unknown. In this study, we systematically analyzed the actomyosin cytoskeleton and regulators downstream of the small GTPase RhoA in the control of microglia shape and function. Our results reveal that (i) Myh9 controls cortical tension levels and affects microglia protrusion formation, (ii) cofilin‐mediated maintenance of actin turnover regulates microglia protrusion extension, and (iii) Myh10 influences microglia inflammatory activation. Overall we uncover molecular pathways that regulate microglia morphology and identify type‐II myosins as important regulators of microglia biology with differential roles in the control of cell shape (Myh9) and functions (Myh10).