bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sanggenol L Induces Apoptotic Cell Death by Activating the Caspase/AIF/p53 Signaling Pathway in Breast Carcinoma
Authors: Pan Li , Hao Zhiqiang , Huang Wei , Yang Lu , Yu Guozheng 
Published: December 2024

Background Breast carcinoma is a deadly malignancy with a greater risk of morbidity and death, which has contributed significantly to the sharp rise in cancer cases. It has a recognized impartial effect on women. The need for a variety of treatment choices is prompted by the current therapy modules’ lack of effectiveness and adverse effects. Purpose In the present investigation, we examined the therapeutic value of Sanggenol L (San L) in human breast carcinoma cells. San L impacts on carcinoma of the breast cells are not fully understood in terms of its molecular and cellular pathways. Methods In order to assess the effectiveness of San L, this investigation employs a number of qualitative approaches, such as cytotoxicity analysis, sulforhodamine B (SRB), 4′,6-diamidino-2-phenylindole (DAPI), reactive oxygen species (ROS), and cell cycle assessment, which were conducted using breast cancer BT-474 cells. Additionally, cell death caused by San L through caspase-dependent and independent apoptosis has been investigated with western blot analysis. Results and Conclusion San L therapy significantly diminished the ability of BT-474 breast carcinoma cells to proliferate and impeded colony formation while having no impact on HBL-100 normal breast epithelial cells in a dose- and time-dependent way. San L treatment resulted in cell cycle arrest and cell death in breast carcinoma cells, which were characterized by acridine orange/ethidium bromide (AO/EtBr), DAPI, and flow cytometry at time-dependent way. The present study also explained that the half-maximal inhibitory concentration (IC50) concentration of (21 and 17.3 µM/mL) San L treatment induced caspase-dependent apoptosis [enhanced the expression of Bax and decreased the expression of B-cell lymphoma 2 (Bcl-2), procaspase-3, -8, and -9], stimulation of caspase-independent apoptosis [upregulation of apoptosis-inducing factor (AIF) and endonuclease G (Endo G) on cytosol], and suppression of cell cycle [downstairs regulation of cyclin-dependent kinase 1/2 (CDK1/2), cyclin D1, cyclin E, or upregulation of p53] in breast tumor cells. According to these findings, San L causes both caspase-dependent and independent apoptosis by triggering p53 in breast carcinoma cells.
Investigating insertional code-mixing in Italian migrants in the United Kingdom: A comparative approach
Authors: Di Salvo Margherita 
Published: December 2024

Aims: This article investigates English lexical insertions in a corpus collected with first- and second-generation Italian migrants in two English cities, namely Bedford and Cambridge. It aims to establish whether, and to what extent, two Italian heritage communities share the same patterns of language alternation. Methodology/approach: I describe the frequency and type of lexical insertions to verify whether the study of lexical insertions or insertions of multiword units can shed light on the linguistic repertoire of each of the communities. The two Italian communities share many social features but differ in the ways Italians integrated or not into the host society. Data analysis: The dataset used for this contribution was collected through qualitative interviews with Italian migrants belonging to three different generations: first, second, and intermediate generations. The corpus used in this paper consists of approximately 50 hours of speech (35 hours for Bedford and 15 hours for Cambridge). I correlated the insertions of single lexemes with semantic and pragmatic specificity from a quantitative and qualitative perspective. Conclusions/results: The results provided evidence that insertion is first influenced by specificity as already Backus argued. External parameters such as migrants’ employment, ethnic composition of the social network, the size of the migrant community, and its social visibility do not, therefore, affect the borrowability of the lexemes belonging to specific domains, the frequency of which leads to their entrenchment as English lexemes even when speakers adopt the monolingual mode in Italian or dialect. The two communities do, however, differ in terms of the matrix language into which the English insertion is embedded: Italians prefer Italian as the matrix language in Cambridge but not in Bedford. Originality: The study confirms how similar Italian communities can be characterized by different linguistic usage and by a different linguistic repertoire.
Superior persistence of ustekinumab compared to anti-TNF in vedolizumab-experienced inflammatory bowel diseases patients: a real-world cohort study
Authors: Chiu Horng-Yih , Kuo Chia-Jung , Lai Ming-Wei , Wu Ren-Chin , Chen Chien-Ming , Chiu Cheng-Tang , Pan Yu-Bin , Chiu Cheng-Hsun , Le Puo-Hsien 
Published: December 2024

Abstract
Background/Aims
The increasing use of biologic therapies for moderate to severe inflammatory bowel disease (IBD) highlights the importance of optimal treatment sequencing, particularly after vedolizumab (VDZ) exposure. Studies comparing the effectiveness of ustekinumab (UST) and antitumor necrosis factor (anti-TNF) agents post-VDZ are limited.

Methods
This retrospective study analyzed VDZ-experienced IBD patients treated with UST or anti-TNF (adalimumab and infliximab) from May 2019 to January 2024. We conducted a comparative analysis of the 52-week treatment persistence between UST and anti-TNF therapies, while also identifying independent predictors that influence 52-week persistence.

Results
The study included 110 participants, with 40 diagnosed with ulcerative colitis (UC) and 70 with Crohn’s disease (CD). Demographics were comparable across treatment groups. The primary discontinuation reason for VDZ was secondary non-response. Kaplan-Meier analysis revealed that UST demonstrated superior 52-week persistence in overall IBD, CD and UC patients, compared to anti-TNF. Cox regression analysis also showed UST’s superiority in overall IBD (HR: 0.15, 95% CI: 0.05–0.45, p < 0.001), CD (HR: 0.09, 95% CI: 0.01–0.68, p = 0.02), and UC (HR: 0.28, 95% CI: 0.08–0.996, p = 0.049). The independent predictors for 52-week treatment persistence are Crohn’s disease (Odds Ratio: 7.151, 95% CI: 1.763–28.995, p = 0.006) and UST treatment (Odds Ratio: 7.912, 95% CI: 1.789–34.992, p = 0.006). Notably, UST required more frequent dosing adjustments than anti-TNF, although both treatments exhibited comparable safety profiles.

Conclusions
UST demonstrated superior 52-week treatment persistence in IBD patients previously treated with VDZ compared to anti-TNF agents, albeit with a need for more frequent dose adjustments.
Onco-mNGS facilitates rapid and precise identification of the etiology of fever of unknown origin: a single-centre prospective study in North China
Authors: Liu Bingbing , Yu Tengfei , Ren Ruotong , Wu Na , Xing Nanshu , Wang Jingya , Wu Wenjie , Cao Xuefang , Zhang Jingping 
Published: December 2024

Abstract
Objectives
Delayed diagnosis of patients with Fever of Unknown Origin has long been a daunting clinical challenge. Onco-mNGS, which can accurately diagnose infectious agents and identify suspected tumor signatures by analyzing host chromosome copy number changes, has been widely used to assist identifying complex etiologies. However, the application of Onco-mNGS to improve FUO etiological screening has never been studied before.

Methods
In this single-centre prospective study, we included 65 patients with classic FUO, who were randomly divided into control group (sample cultivation) and mNGS group (cultivation + Onco-mNGS). We analyzed the infectious agents and symbiotic microbiological, tumor and clinical data of both groups.

Results
Infection-related pathogenic detection efficiency rose from 25% (control group) to 48.48% (experimental group). Seven patients with chromosome copy number changes had later been confirmed tumors, indicating a 100% of clinical concordance rate of Onco-mNGS for tumors. In addition, the time frame for diagnosing or ruling out infection/tumor with Onco-mNGS had greatly reduced to approximately 2 days, which was 7.34 days earlier than that in the control group.

Conclusions
Onco-mNGS is an ideal rapid diagnostic aid to assist improving the early diagnostic efficiency of FUO-associated diseases.
(1, 3)-beta-D-Glucan in bronchoalveolar lavage fluid: a useful biomarker in diagnosis of invasive pulmonary infection caused by Hormographiella aspergillata?
Authors: Ye Haiyan , He Jinhui , Huang Jing , Chu Patrick , Liu Junru , Poon Rosana Wing-Shan , Xing Fanfan , Lo Simon Kam-Fai , Lau Ricky Wing-Tong , Chan Jasper Fuk-Woo , Lau Susanna Kar-Pui , Chiu Kelvin Hei-Yeung 
Published: December 2024

AbstractHormographiella aspergillata is a rare hyaline mold causing invasive fungal infection in humans, until the frequent use of antifungal prophylaxis in immunocompromised hosts. Due to the high mortality of H. aspergillata infection, early recognition and treatment are crucial. Previous case reports suggested that serum (1,3)-beta-D-Glucan (BG) is one of the diagnostic aids for H. aspergillata infection. Here we report for the first time a case of pulmonary H. aspergillata infection with a negative serum BG but positive bronchoalveolar lavage fluid (BAL) BG. This may suggest that BAL BG is a useful and additional microbiological marker for prompt identification of this fatal invasive fungal infection (IFI). But it should be interpreted together with the clinical presentation, imaging, and other laboratory results.
Bronchial artery pseudoaneurysm presenting with hemoptysis and hemothorax after pulmonary lobectomy: a case report and literature review
Authors: Zhang Wei-Hao , Jin Cheng-Yan , Qi Xiao , Zhang Guang-Xin 
Published: December 2024

Abstract
Background
Bronchial artery pseudoaneurysm is a rare vascular disorder, and cases of bronchial pseudoaneurysms reported after lung surgery are even rarer. The number of reported cases is very limited due to the unclear pathogenesis, lack of diagnostic criteria and treatment guidelines, and nonspecific clinical manifestations.

Case presentation
The paper reports a case of a patient with primary lung adenocarcinoma who developed hemoptysis, chest and back pain, and right hemothorax after lobectomy. Due to the lack of experience in treatment, we suspected that the above symptoms were caused by postoperative broncho-vascular fistula. The second thoracotomy and bronchoscopy examination did not reveal any obvious hemorrhage or fistula, and a subsequent bronchial arteriography confirmed the presence of a pseudoaneurysm in the right main bronchial artery. Later, the patient underwent transcatheter bronchial artery embolization and was followed up for half of a year after the operation without experiencing any of the previously mentioned symptoms.

Conclusions
The case reports the successful cure of bronchial artery pseudoaneurysm after pulmonary lobectomy through bronchial artery embolization.
Evans syndrome as a presentation in systemic lupus erythematous, coexisting with Hashimoto’s thyroiditis and pernicious anemia: a case report
Authors: Mansour Maryam , Shamasnah Arwa , Alsaadi Deema , Abu Saif Saja , krama Akram 
Published: December 2024

Abstract
Background
Evans syndrome is a rare disorder characterized by the simultaneous or sequential combination of autoimmune hemolytic anemia and immunological thrombocytopenia, together with a positive direct antiglobulin test. This syndrome, which can be primary or secondary, is a rare initial manifestation of autoimmune diseases, notably systemic lupus erythematosus, with 1.7–2.7% of patients with systemic lupus erythematosus developing secondary Evans syndrome, sometimes preceding the onset of systemic lupus erythematosus symptoms.

Case presentation
A 47-year-old Middle Eastern female presented with symptoms including shortness of breath, chest pain, and weakness. Physical examination revealed pallor, pale conjunctiva, icteric sclera, tachycardia, and tachypnea. She was diagnosed with Evans syndrome owing to hemolytic anemia, thrombocytopenia, and a positive Coombs test, where initial resistance to treatment prompted intensive therapy with methylprednisolone, intravenous immunoglobulin, and rituximab. Subsequent identification of systemic lupus erythematosus on the basis of positive anti-nuclear antibodies and anti-double-stranded deoxyribonucleic acid antibodies led to treatment with mycophenolate mofetil and hydroxychloroquine. Further evaluations uncovered Hashimoto’s thyroiditis and pernicious anemia, necessitating thyroxine supplementation and vitamin B12 injections.

Conclusion
Hematologic abnormalities play a crucial role in the diagnostic framework of systemic lupus erythematosus. This case highlights a patient initially diagnosed with Evans syndrome, revealing an underlying systemic lupus erythematosus. The presentation underscores the significance of hematologic manifestations as integral components of the diagnostic framework for autoimmune diseases, emphasizing the intricate relationship between Evans syndrome and systemic lupus erythematosus.
Dynamics of affect modulation in neurodevelopmental disorders (DynAMoND) – study design of a prospective cohort study
Authors: Bayas Maximilian , Kockler Tobias D. , Ramos-Quiroga Josep Antoni , Caller Silvia Muñoz , Fadeuilhe Christian , de Girolamo Giovanni , Iozzino Laura , D’Addazio Miriam , Haavik Jan , Halmøy Anne , Hellum Karin Schiøler , Kolle Joakim Njaastad , Osnes Berge , Lundervold Astri J. , Perroud Nader , Hasler Roland , De Almeida Mélanie Teixeira , Ebner-Priemer Ulrich W. , Thanarajah Sharmili Edwin , Schiweck Carmen , Matura Silke , Repple Jonathan , Reif Andreas , Aichholzer Mareike 
Published: December 2024

Abstract
Background
Attention-deficit/hyperactivity disorder (ADHD) is a common neuro-developmental disorder that often persists into adulthood. Moreover, it is frequently accompanied by bipolar disorder (BD) as well as borderline personality disorder (BPD). It is unclear whether these disorders share underlying pathomechanisms, given that all three are characterized by alterations in affective states, either long or short-term. BD is characterized by infrequent but intense mood shifts, while ADHD and BPD involve more dynamic emotional fluctuations. It is yet to be determined whether these disorders represent distinct phenomena or different points on a spectrum of affective dysregulation.

Methods
This study seeks to distinguish the emotional dysregulation of BPD, ADHD, and BD by using digital phenotyping, a measurement burst electronic-diary method with different sampling rates, and accelerometry to measure participants’ activity. Our study will include 480 participants aged 14 to 50 (120 each from BPD, ADHD, BD, and healthy control groups) from five European sites. Participants’ smartphones will provide continuous data on their digital phenotypes, i.e., by indicators of physical activity and communication, for one year, along with daily evening ratings of mood and sleep. Moreover, five intensive measurement periods of five days each, called measurement bursts, will occur throughout the year, with electronic diaries asking participants to report on mood, self-esteem, impulsivity, life events, social interactions, and dysfunctional behaviors ten times a day. Moreover, participants will wear activity sensors during the five measurement bursts. Statistical analysis aims to identify whether affective dysregulation aspects share or differ across disorders. Specifically, data analysis aims to investigate the differences in parameters of affect fluctuation such as attractor strength and variability between disorders and to test the association of genetic risk factors for psychiatric disorders and resilience factors with critical parameters of affect modulation.

Discussion
The results of this study offer the potential to link patients’ external exposures with their affective state, reduce misdiagnosis, and determine the best timing for therapeutic interventions. Potential limitations of the study include insufficient recruitment of patients and drop-outs due to various protocol violations.

Trial registration
Study code: DRKS00028917, registered 27.07.2022, https://drks.de/search/de/trial/DRKS00028917.
Approach to the Patient With Thyroid Cancer: Selection and Management of Candidates for Lobectomy
Authors: Robenshtok Eyal , Bachar Gideon , Ritter Amit 
Published: December 2024

Abstract
Thyroid lobectomy has gained increasing popularity over the past decade as a treatment for differentiated thyroid cancer, largely due to a rise in the diagnosis of low-risk cancers and evidence showing no benefit from radioiodine in low-risk disease. Multiple studies have confirmed lobectomy as an effective and safe option. Its advantages over total thyroidectomy include lower complication rates and a reduced need for lifelong levothyroxine (LT4) therapy. Recent research has broadened the indications for lobectomy, extending its use to patients with contralateral benign nodules and several “adverse” histological features, such as minimal extrathyroidal extension, small lymph node metastases, or multifocality—provided these are of minimal size. For patients with follicular thyroid carcinoma, who typically undergo surgery for Bethesda III-IV cytology, minimally invasive disease should be treated with lobectomy alone. In patients with angioinvasion, the risk of metastasis increases with the number of vessels involved (&gt;2-3 vessels), larger tumor size, and advanced patient age. Following surgery, current evidence supports a TSH target within the normal range, reducing the need for LT4 treatment to only 30% of patients. Follow-up is based on neck ultrasound because thyroglobulin levels have limited value in detecting recurrence. In cases where further treatment is required, completion thyroidectomy is as safe as upfront total thyroidectomy. Overall, lobectomy is the preferred treatment option for many, if not most, differentiated thyroid cancer patients with low to low-intermediate risk disease, offering lower complication rates, reduced need for LT4 therapy, and excellent long-term outcomes.
Tinjauan Pustaka Sistematis: Tantangan Dan Faktor-Faktor Pengembangan Kesiapan Forensik Digital
Authors: Rochmadi Tri , Fadlil Abdul , Riadi Imam 
Published: December 2024

Teknologi informasi telah mengubah hampir segala lini kehidupan dari konvensional ke digitalisasi. Digitalisasi yang begitu cepat, menimbulkan permasalahan pada serangan siber yang belum diimbangi oleh penanganan insiden siber. Kesiapan forensik digital menjadi hal penting bagi organisasi agar proses penanganan insiden lebih efektif dan efisien. Tujuan penelitian ini adalah mengidentifikasi faktor-faktor dan tantangan terkait topik kesiapan digital forensik. Metode yang digunakan dalam penelitian ini menggunakan metode tinjauan pustaka sistematis. Hasil yang didapatkan pada penelitian ini memberikan informasi jurnal yang paling banyak mempublikasikan topik kesiapan forensik digital, obyek penelitian, metode yang digunakan, standarisasi yang diintegrasikan, faktor-faktor dan tantangan dalam pengembangan kesiapan forensik digital. Penelitian ini bisa menjadi rujukan peneliti akademisi ataupun praktisi pada bidang forensik digital ataupun pengembang aplikasi.
Kata kunci: tinjauan pustaka sistematis, kesiapan forensik digital, ISO/IEC 27043, COBIT.
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Abstract
Information technology has changed almost all lines of life from conventional to digitalization. Rapid digitalization has led to problems with cyber-attacks that the handling of cyber incidents has not matched. Digital forensic readiness is important for organizations to make the incident-handling process more effective and efficient. The purpose of this research is to identify factors and challenges related to the topic of digital forensic readiness. The method used in this research is a systematic literature review. The results obtained in this study provide information on the journals that publish the most digital forensic readiness topics, research objects, methods used, standardization integrated, factors, and challenges in developing digital forensic readiness. This research can be a reference for academic researchers or practitioners in digital forensics or application developers.
Keywords: systematic literatur review, digital forensic readiness, ISO/IEC 27043, COBIT.
Evaluation of IT Governance at Islamic Boarding Schools in the Special Region of Yogyakarta based on the COBIT 5 Framework
Authors: Mardlian M. Sa’id Abdurrohman Kunta , Nuruzzaman Muhammad Taufiq , 'Uyun Shofwatul , Sugiantoro Bambang 
Published: December 2024

Nowdays, almost all aspects of company operations are supported by information technology systems. Many pesantren have utilized information technology in supporting operational activities such as: using computers, accessing the internet, having a website, and managing information technology systems. The purpose of this study is to implement the COBIT 5 framework in the DSS (Deliver, Service, Support) domain in evaluating information technology governance and calculate the capability level value and gap analysis at Islamic Boarding Schools. The results of the Capability Level Analysis in the COBIT 5 DSS Domain obtained from 42 Islamic Boarding Schools showed that DSS01, DSS02, and DSS03 on average were at level 2, DSS04, DSS05, DSS06 on average at level 1. The results of the Gap Analysis in the COBIT 5 DSS Domain show that in DSS01, DSS02, and DSS03 the average GAP is 3, in DSS04, DSS05, and DSS06 the average GAP is 4. The result shows that Information Technology Governance in Islamic Boarding Schools in the Special Region of Yogyakarta based on an assessment with the COBIT 5 Framework is still low status and needs to be improved, as evidenced by the low Capability Level Value and the high GAP Value.
Dramatic response to Evinacumab in a North Indian girl with homozygous familial hypercholesterolemia
Authors: Arora Sonali , Banerjee Sayan , George Arun , Bala Anju , Thingnam Shyam Kumar Singh , Rohit Manoj Kumar , Dayal Devi 
Published: December 2024

Abstract

Objectives
Homozygous familial hypercholesterolemia (HoFH) is a rare inherited disorder of lipoprotein metabolism associated with significant morbidity and early mortality. The conventional management with lipid-lowering drugs and lipoprotein apheresis is unable to consistently achieve guidelines recommended low-density lipoprotein cholesterol (LDL-C). We aim to describe the efficacy of Evinacumab, a recently approved monoclonal antibody, in lowering LDL-C in an Indian girl with HoFH.

Case presentation
A 16-year-old girl was diagnosed with HoFH at age two years after the appearance of cutaneous and tendo-calcaneus xanthomas. Genetic testing revealed a pathogenic homozygous deletion in the LDL receptor (LDLR) gene. Despite lifestyle measures, a low-fat diet, and maximum doses of atorvastatin and ezetimibe, her average LDL-C level remained 320.3 mg/dL over the past decade. Her caregivers did not accept LDL-apheresis. She also did not respond to Evolocumab therapy. The patient developed progressive calcific aortic stenosis and concentric left ventricular hypertrophy, necessitating aortic valve replacement surgery at age 16 years. The recent addition of Evinacumab to her lipid-lowering drug regimen, resulted in a significant LDL-C reduction of 76.16 %, bringing levels down to 82 mg/dL. These levels were sustained over the last four months.

Conclusions
Evinacumab offers a promising option for managing high-risk and difficult-to-treat HoFH patients. This is the first Indian child receiving Evinacumab for HoFH.