bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resource-constrained Time-cost-quality-energy-environment Tradeoff in Project Scheduling by Considering Blockchain Technology: A Case Study of Healthcare Project
Authors: Lotfi Reza , Kargar Bahareh , Gharehbaghi Alireza , Hazrati Hanif , Nazari Sima , Amra Mohsen 
Published: December 2021

Abstract
Blockchain Technology (BCT) is expanding day by day and is used in all pillars of life and projects. In this research, we survey applicable of BCT in project management for the first time. We presented a Resource-Constrained Time-Cost-Quality-Energy-Environment tradeoff problem in project scheduling by considering BCT (RCTCQEEBCT). We utilize hybrid robust stochastic programming, worst case and Conditional Value at Risk (CVaR) to cope with uncertainty and risks. This type of robustification and risk-averse is presented in this research. A real case study is presented in a healthcare project. We utilize GAMS-CPLEX to solve the model. Finally, we analyze finish time, conservative coefficient, the confidence level of CVaR and the number of scenarios. The most important research result is that applying BCT decreases cost, energy, and pollution and increases quality. Moreover, the total gap between RCTCQEEBCT and without BCT is approximately 2.6%. When compacting finish time happens or if the conservative coefficient increases to 100%, costs, energy, and pollution environment increase, but quality decreases. If the confidence level of CVaR increase, the cost, energy and environment function functions grow up and quality is approximately not changed.
Monosialoganglioside GM1 Deficiency Inhibits the Neurotrophic Effects of GDNF by Disrupting Lipid Raft Assembly
Authors: Jiang Shimin , Zhou Tai , Zhang Kejia , Zhou Yao , Wang Zhongcheng , Li Li 
Published: December 2021

Abstract
Recent studies have shown that monosialoganglioside GM1 deficiency can inhibit the signal transduction process of glial cell line-derived neurotrophic factor (GDNF), which plays an important role in the pathogenesis of Parkinson's disease (PD). However, its specific mechanism still needs to be explored. We inhibited the expression of GM1 by treating cells with D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP). CCK-8 assay, EdU cell proliferation assay and Western blot assay were used to evaluate the effect of GM1 deficiency on the proliferation and differentiation of SH-SY5Y cells induced by GDNF and on the GDNF-RET signaling pathway. Lipid rafts were isolated by Triton X-100 solubilization and OptiPrepTM density gradient centrifugation. The alterations of lipid raft assembly and the translocation of RET into lipid rafts were evaluated after PDMP treatment. We found that PDMP treatment inhibited the proliferation and differentiation of SH-SY5Y cells induced by GDNF and reduced the phosphorylation of RET and its downstream signaling molecules Erk and Akt. In addition, after PDMP treatment, caveolin-1 and flotillin-1, the prototypical markers of lipid rafts, diffused from lipid rafts to non-lipid raft microdomains, and GDNF-induced RET translocation into lipid rafts was also reduced. These alterations could be partially reversed by adding exogenous GM1. Our results suggest that ganglioside GM1 deficiency could compromise the neurotrophic effects and signals downstream of GDNF by altering the assembly of lipid raft membrane microdomains.
5’UTR Variant rs6017916 Regulates SRC Expression and Contributes to the Susceptibility to Ischemic Stroke in the Chinese Han Population
Authors: Yang Jialei , Song Xiaoxiao , Yang Yibing , Yan Yan , Liang Baoyun , Zhu Lulu , Gu Lian , Su Li 
Published: December 2021

Abstract
Previous studies reported that the SRC protein was involved in a variety of pathological mechanisms related to ischemic stroke (IS). In this study, we conducted a genetic association study between rs6017916 within the 5’UTR region of SRC gene and IS susceptibility. A total of 533 IS patients and 531 healthy controls were recruited to participate in the current study. The sequenom MassARRAY technology platform was used for genotyping. The quantitative polymerase chain reaction (qPCR) was conducted to detect SRC mRNA expression. The dual luciferase reporter system was used to verify the regulation of rs6017916 on SRC mRNA expression. Results showed that SRC mRNA expression was significantly increased in IS patients than that in controls (P<0.001). Receiver operating characteristic curve (ROC) analysis demonstrated that the signature of SRC mRNA expression differentiated between controls and IS patients with an area under the curve (AUC) of 0.935 corresponding to a specificity of 0.820 and sensitivity of 0.920. Genetic association analysis showed that rs6017916 was significantly associated with IS susceptibility under multiple genetic models, including additive [OR (95% CI)=0.76 (0.60,0.96), Padj=0.019] and dominant [OR (95% CI)=0.75(0.58,0.98), Padj=0.031]. In addition, the dual luciferase reporter system showed that the minor allele C of rs6017916 inhibited luciferase activity compared with the major allele A. In summary, we report that SRC mRNA expression was significantly increased in IS patients and was a potential diagnostic biomarker. Moreover, the 5’UTR variant rs6017916 of SRC was significantly associated with IS susceptibility. And rs6017916 might affect the pathological process of IS by regulating SRC expression.
Continuation Newton methods with deflation techniques and quasi-genetic evolution for global optimization problems
Authors: Luo Xin-long , Xiao Hang 
Published: December 2021

Abstract
The global minimum point of an optimization problem is of interest in engineering fields and it is difficult to be solved, especially for a nonconvex large-scale optimization problem. In this article, we consider the continuation Newton method with the deflation technique and the quasi-genetic evolution for this problem. Firstly, we use the continuation Newton method with the deflation technique to find the stationary points from several determined initial points as many as possible. Then, we use those found stationary points as the initial evolutionary seeds of the quasi-genetic algorithm. After it evolves into several generations, we obtain a suboptimal point of the optimization problem. Finally, we use the continuation Newton method with this suboptimal point as the initial point to obtain the stationary point, and output the minimizer between this final stationary point and the found suboptimal point of the quasi-genetic algorithm. Finally, we compare it with the multi-start method (the built-in subroutine GlobalSearch.m of the MATLAB R2020a environment) and the differential evolution algorithm (the DE method, the subroutine de.m of the MATLAB Central File Exchange 2021), respectively. Numerical results show that the proposed method performs well for the large-scale global optimization problems, especially the problems of which are difficult to be solved by the known global optimization methods.
Cultured and Uncultured Microbial Community Associated With Biogas Production
Authors: Ottoni Júlia Ronzella , Bernal Suzan Prado Fernandes , Marteres Tiago Joelzer , Luiz Franciele Natividade , Santos Viviane Piccin dos , Mari Ângelo Gabriel , Somer Juliana Gaio , de Oliveira Valéria Maia , Passarini Michel Rodrigo Zambrano 
Published: December 2021

Abstract
The search for sustainable development has led countries around the world to seek the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly, in addition to reducing public expenses for the treatment of those wastes. The present study evaluated the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing analysis of isolates recovered from the starter culture, the genera Rhodococcus, Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Luteimonas and Proteiniclasticum were recovered, groups that were not observed in the metabarcoding data. The groups mentioned are involved in the metabolism pathways of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing of isolates, as well as culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated, provide robust and comprehensive information about the microbial communities involved in various processes, including the production of biogas in anaerobic digestion processes.
A New Strategy for Improving the Surface Quality of Ti6Al4V Machined by Abrasive Water Jet: Reverse Cutting with Variable Standoff Distances
Authors: Xiong Jie , Wan Liang , Qian Yi'nan , Sun Shuo , Li Deng , Wu Shijing 
Published: December 2021

Abstract
Titanium alloys are widely used in important structures of aerospace vehicles, but the low thermal conductivity and high chemical activity make them difficult to process. As an untraditional machining technology, abrasive water jet (AWJ) has been proven to be an effective method for this kind of material. Aimed at further improving the cutting performance, reverse cutting with variable standoff distance (SOD) strategy was put forward, and experiments of titanium alloy Ti6Al4V machined by AWJ were conducted. The influence of SOD with different reverse cutting types on the kerf quality was studied to obtain the optimal SOD combinations. Ra, Sa and kerf taper were used to evaluate the quality of the machined surface. Moreover, the results of reverse cutting at the same speed and efficiency and single cutting at the constant SOD were compared and analyzed. It was found that the proposed strategy results in higher kerf quality in the aspect of surface roughness, compared to the single cutting. To be more specific, for the reverse trimming cutting, the improvements of Ra and Sa can reach up to 62.8% and 73.1% respectively under the condition of the SOD of the second cutting is 8mm. Furthermore, the kerf taper can be reduced 26.1% when the SOD of the second cutting is 2mm. With respect to the reverse deepening cutting, even the traverse speed of reverse cutting is set as twice as that of a single cutting, the kerf quality is still better. Additionally, when the SOD of the second cutting is 4mm, the improvements of Ra and Sa can reach up to 51.7% and 14.9%, respectively, and the kerf taper is reduced by 20.2%. This study provides a new method for improving the machined surface quality of hard materials, especially for Ti6Al4V.
Evaluation of: MOSSA, MOALO, MOVO and MOGWO Algorithms in Green Machining for High Production Performance in Turning of X210Cr12 Steel
Authors: NOUIOUA Mourad , LAOUISSI Aissa , BLAOUI Mohamed Mossaab , HAMMOUDI Abderzzak , YALLESE Mohamed Athmane 
Published: December 2021

Abstract
The current study investigates the Wet and MQL machining, when turning of X210Cr12 steel, using a multilayer-coated carbide insert (GC-4215) with various nose radius, the consideration of the tool geometry with different cooling modes allow as to assess the comportment of the machined steel against the cutting combinations. The response surface methodology (RSM) has been used for regression analysis and to evaluate the contribution of the cutting parameters on surface roughness, tangential force and cutting power using ANOVA analysis. The developed models have been used to predict the studied output factors according to the selected cutting parameters for wet and MQL machining. A comparative between the cooling techniques have been established to determine the most effective technique in terms of part quality, lubricant consumption and power consumption. Finally, four new optimization technics have been used for the process optimization using the MQL models for an environment-friendly machining.
A Novel Optimized Formulation for Cooling Tower Effluent Disinfection: Tarasht Power Plant Case Study
Authors: Sohrabi Ehsan , Sohrabi Somayeh , Iranshahi Davood , Sarmadi Majid , Moraveji Mostafa Keshavarz 
Published: December 2021

Abstract
Since the operating conditions of the cooling tower units provide such a suitable environment for the growth of bacteria and algae, taking measures for the disinfection process is strongly recommended. The cooling tower unit is proceeded by RO and the disinfection strategy is sodium hypochlorite, combined with this new formulation. The sampling result revealed that bacteria counts from the effluent of the cooling tower are 2600 CFU/ml while the microbial load of the water in the pond is 220 CFU/ml. Herein, two type formulations are introduced for cooling tower output stream while the pond water comes along with a NaOCl injection. The dosage of A: Glucose Oxidase, B: ammonium persulfate, and C: Amoxicillin, and D: the order of use are chosen as the four independent variables whose effects and their binary interactions on microbial load and disinfection efficiency were investigated via Box–Behnken design (BBD) combined with response surface methodology (RSM). The ANOVA results show that the most significant variable is amoxicillin in the presence of bleach. Meanwhile, high values for R2 > 0.99 and the model F-value 45.64 in addition to diagnostic tests confirmed the reliability of the model. Several solutions have been introduced as optimum formulations. The pre-scale up and economic considerations have been included.
Canonical Wnt Signaling Inhibition in Renal Cell Carcinoma Bone Metastasis: Immunohistochemical Study of DKK1 and LRP5 Expression
Authors: Huang Zixiong , Du Yiqing , Yin Huaqi , Wang Gongwei , Xu Tao 
Published: December 2021

Abstract
Introduction & Objectives: Canonical Wnt signaling (Wnt/β-catenin signaling) maintains the bone homeostasis by promoting the osteoblastic activities. The inhibitory factor, Dickkopf (DKK)1, enhances the bone resorption, especially in malignancies. The low density lipoprotein related protein (LRP) 5 is a component of membranous co-receptor of Wnt/β-catenin signaling and is also involved in serum low density lipoprotein cholestrol (LDL-C) level regulation. The clear cell renal cell carcinoma bone metastasis (ccRCC-BM) is characterized by osteolytic bone resorption. Whether and how Wnt/β-catenin signaling plays roles in regulating the invasion, metastasis and osteolytic process of ccRCC to bone remain unclear. This study investigated the expression of DKK1, LRP5 proteins in primary and metastatic lesions of RCC-BM. The therapeutic potential of Wnt/β-catenin signaling target medication was also evaluated.Materials & Methods: ccRCC-BM patients with paired samples of primary and metastatic lesions were selected. ccRCC patients without any metastasis (ccRCC-only) were set as control. Slides of paraffin-embedded tissue underwent immunohistochemical staining with monoclonalanti-DKK1 antibody and polyclonal anti-LRP5 antibody. Semi-quantitatively scoring according to staining intensity was performed. The staining results in the renal tissue adjacent to RCC, the primary RCC lesions (with BM or without BM), and the RCC-BM lesions were recorded. The expression difference was analyzed by univariate analysis of variance (ANOVA).Results: The expression of DKK1 was significantly different amid renal tissue adjacent to RCC, primary RCC and RCC-BM tissues (p< 0.001). The expression of DKK1 in primary RCC was significantly lower than that in renal tissue adjacent to RCC (p<0.001). No difference was found between ccRCC-BM group and ccRCC-only group. DKK1 expression in bone metastasis was significantly higher than that in primary tumor (p < 0.001). The expression of LRP5 in the primary tumor of ccRCC-BM group was significantly lower than that of adjacent renal tissue (p<0.01). Tendency of decreasing expression was found between primary lesion of ccRCC-BM group and primary lesion of ccRCC-only group (p=0.073). In bone metastasis, the expression of LRP5 protein was not significantly different from that in adjacent renal tissue and RCC primary lesion.Conclusions: A "rebound" of DKK1 expression was found in bone metastasis lesions. Along with the decreasing LRP5 expression in primary lesions of RCC-BM patients, this suggests that the canonical Wnt signaling (Wnt/β-catenin signaling) is inhibited during the bone metastasis process in ccRCC. The overexpression of DKK1 and the down-regulation of LRP5 receptor are involved.
Linear Dose Response of Acrocentric Chromosome Associations (Aca) To Gamma Irradiation In Human Lymphocytes
Authors: Samarth Ravindra M , Gandhi Puneet , Chaudhury Nabo Kumar 
Published: December 2021

Abstract
Purpose: The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose-response relation with low doses of gamma irradiation in lymphocytes. Methods: Peripheral blood collected from three healthy donors were irradiated with 0, 0.1, 0.25, 0.5, 0.75, and 1 Gy gamma radiation. Chromosomal preparations were made after 48 hrs culture as per the standard guidelines. Results: The average number of ACA and ACA % were increased significantly with an increase in a dose. The D-G and D-D type of association was most prominent and showed a dose-dependent increase. The ACA frequency in irradiated lymphocytes showed an increase concerning the dose. The fitted regression equation was y=0.4759x+0.1663 (R2=0.9635; p=0.0005). An assessment of dicentric chromosomes (DC) was carried for the same slides. The correlation curve was prepared for ACA frequencies versus DC frequencies, resulting in a regression equation as y=8.659x+0.2.37 (R2=0.8275; p=0.0119). Conclusion: Our results showed an increase in frequencies of ACA in irradiated lymphocytes with an increase in radiation dose and followed a similar linear trend with DC frequency, thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry especially for low radiation doses.
Neuregulin-1 Regulates the Conversion of M1/M2 Microglia Phenotype via ErbB4-dependent Inhibition of the NF-κB Pathway
Authors: Ma Yuqi , Fan Peixia , Zhao Rui , Zhang Yinghua , Wang Xianwei , Cui Weigang 
Published: December 2021

Abstract
BackgroundThe inflammatory response caused by microglia in the central nervous system plays an important role in Alzheimer's disease. Neuregulin-1 (NRG1) is a member of the neuregulin family and has been demonstrated to have anti-inflammatory properties. The relationship between NRG1, microglia phenotype and neuroinflammation remains unclear.Materials and MethodsBV2 cells were used to examine the mechanism of NRG1 in regulating microglia polarization. Neuronal apoptosis, inflammatory factors TNF-α and iNOS, microglia polarization, ErbB4 and NF-κB p65 expression were assessed.ResultsWe found that exogenous NRG1 treatment or overexpression improved microglial activity and reduced the secretion of the inflammatory factors TNF-α and iNOS in vitro. The expression of Bax in SH-SY5Y neuron cells incubated with medium collected from the NRG1 treatment group decreased. Additionally, our study showed that NRG1 treatment reduced the levels of the M1 microglia markers CD120 and iNOS and increased the levels of the M2 microglia markers CD206 and Arg-1. Furthermore, we observed that NRG1 treatment attenuated Aβ-induced NF-κB activation and promoted the expression of p-ErbB4 and that knockdown of ErbB4 abrogated the effects of NRG1 on NF-κB, Bax levels and M2 microglial polarization. Conclusion﻿NRG1 inhibits the release of inflammatory factors in microglia and regulates the switching of the M1/M2 microglia phenotype, most likely via ErbB4-dependent inhibition of the NF-κB pathway.
Serum Uric Acid to HDL-Cholesterol Ratio is Associated with New-Onset NAFLD in Lean Chinese People with Normal Blood Lipid Levels: a Longitudinal Cohort Study
Authors: Zhu Wentao , Liang An , Shi Pei , Yuan Song , Zhu Ying , Fu Jiwei , Zheng Ting , Wen Zhilong , Wu Xiaoping 
Published: December 2021

Abstract
Background: Nonalcoholic fatty liver disease (NAFLD) is commonly occurred in the non-obese individuals. The serum uric acid (UA) to high-density lipoprotein cholesterol (HDL-c) ratio (UHR) is considered as a predictive factor of NAFLD. However, it is still difficult to confirm the correlation in lean Chinese people with normal blood lipid levels. It is aimed to analyze the correlation of UHR with NAFLD among lean Chinese population without dyslipidemia and compared UHR with other predictors in this study.Methods: 9838 lean people without NAFLD were included in a retrospective cohort study. NAFLD was diagnosed by liver ultrasound.Results: A total of 9,838 lean patients with normal blood lipid levels were included in the final study. During the five-year follow-up period, the overall prevalence of NAFLD was 8.7%. across the quintile 1, 2, 3, 4 and 5 of UHR, the prevalence of NAFLD among lean patients was increased from 2.4%, 5%, 7.9%, 10.3% to 17.8%. After adjustment for age, markers of liver and kidney function, gender and metabolic indicators, multivariate cox proportional hazard regression analysis demonstrated that the hazard ratio(HR) was 1.99(1.43-2.73) in highest UHR (quintile 5) compared with lowest UHR(quintile 1). The area under the curve(AUC) of UHR (0.690) was higher than in UA (0.666) and HDL-c (0.636), which showed that the predictive ability of the UHR to new-onset NAFLD was better than serum uric acid and HDL-c. Even within the normal range of UA and HDL-c levels, UHR was independently associated with NAFLD, and HR (95% confidence interva, 95%CI) for NAFLD in Quintile 5 of UHR was 6.74(4.32-10.53). Compared with other significant predictors, the AUC value of UHR(0.67) was similar to that of low-density lipoprotein cholesterol(LDL-c)/HDL-c ratio(0.68), non-high-density lipoprotein cholesterol (NHDL-c)/HDL-c ratio(0.68) and alanine aminotransferase (ALT)/ aspartate aminotransferase (AST) ratio(0.7), and superior to that of LDL-c (0.63), remnant cholesterol (RC,0.59), albumin(ALB)/alkaline phosphatase(ALP) ratio(0.61). The sensitivity of UHR (70.5%) was the highest among all indicators. In the subgroup of ALT, the AUC of UHR was 0.70, which was the highest among all predictors in subjects with ALT< 40. For subjects with elevated ALT levels (ALT > 40 U/L), there was no statistical significance among RC (P=0.441), ALB/ALP (P=0.419) and ALT/AST(P=0.159). In contrast, UHR's performance in predicting NAFLD was meaningful and reliable (AUC=0.61, p<0.001).Conclusions: UHR serve as an inexpensive and reliable predictor of NAFLD in lean Chinese people with normal blood lipid levels. It can be used to identify people at high risk of NAFLD.