bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Integrating Data Mining, Network Pharmacology, and Molecular Docking Verification to Investigate the Molecular Mechanism of Traditional Chinese Medicine Prescriptions for Treating Male Infertility
Authors: Bai Xue , Tang Yibo , Li Qiang , Liu Guimin , Liu Dan , Fan Xiaolei , Liu Zhejun , Yu Shujun , Tang Tian , Wang Shuyan , Li Lingru , Zhou Kailin , Zheng Yanfei , Liu Zhenquan 
Published: March 2021

Abstract
Background: Male infertility (MI) affects almost 5% adult men worldwide, and 75% of these cases are unexplained idiopathic. There are limitations in the current treatment due to the unclear mechanism of MI, which highlight the urgent need for a more effective strategy or drug. Traditional Chinese Medicine (TCM) prescriptions have been used to treat MI for thousands of years, but their molecular mechanism is not well defined. Methods: Aiming at revealing the molecular mechanism of TCM prescriptions on MI, a comprehensive strategy integrating data mining, network pharmacology, and molecular docking verification was performed. Firstly, we collected 289 TCM prescriptions for treating MI from National Institute of TCM Constitution and Preventive Medicine for 6 years. Then, Core Chinese Materia Medica (CCMM), the crucial combination of TCM prescriptions, was obtained by the TCM Inheritance Support System from China Academy of Chinese Medical Sciences. Next, the components and targets of CCMM in TCM prescriptions and MI-related targets were collected and analyzed through network pharmacology approach.Results: The results showed that the molecular mechanism of TCM prescriptions for treating MI are regulating hormone, inhibiting apoptosis, oxidant stress and inflammatory. Estrogen signaling pathway, PI3K-Akt signaling pathway, HIF-1 signaling pathway, and TNF signaling pathway are the most important signaling pathways. Molecular docking experiments were used to further validate network pharmacology results. Conclusions: This study not only discovers CCMM and the molecular mechanism of TCM prescriptions for treating MI, but may be helpful for the popularization and application of TCM treatment.
Ahmed Glaucoma Valve Implantation with the Tube Placement in the Ciliary Sulcus: Short Term Results
Authors: Arikan Gul , Akbulut Betul , Utine Canan Asli , Ayhan Ziya , Kaya Mahmut , Ozturk Taylan , Gunenc Uzeyir 
Published: March 2021

Abstract
Purpose: To evaluate the clinical outcomes of pseudophakic/aphakic eyes with uncontrolled glaucoma that underwent Ahmed glaucoma valve implantation with the tube placement in the ciliary sulcus.Methods: Medical records of the patients who underwent Ahmed glaucoma valve implantation through the ciliary sulcus, between December 2017 and June 2019, were reviewed retrospectively. Patients’ age, gender, glaucoma diagnosis, visual acuity, intraocular pressure levels and complications were recorded. Results: Forty-seven eyes of 43 patients with glaucoma were enrolled. The mean age was 54.5 ± 19.9 years (range, 7 - 88 years) at the time of surgery and the mean postoperative follow-up period was 7.9 ± 3.4 months (range, 3 -16 months). The mean preoperative intraocular pressure level was 35.2 ±6.8 mmHg (range, 25-55 mmHg) and it was found as 15.6 ± 5.4 mmHg (range, 9-33 mmHg) at last follow-up visit. Decrease in intraocular pressure level was statistically significant (P < 0.001). At last follow-up visit success was achieved in 41 eyes (87.2 %). Hyphema was the most common postoperative complication and developed in 11 eyes (23.4%) and resolved spontaneously in all of them within one month.Conclusion: In pseudophakic or aphakic eyes with uncontrolled glaucoma, placement of Ahmed glaucoma valve tube in the ciliary sulcus is a safe and effective procedure. Ciliary sulcus can be considered as a potential space during tube shunt surgery in eyes with high risk of tube-corneal touch or corneal decompensation.
Impact of an Integrated Community-based Model of Care for Older People with Complex Conditions on Hospital Emergency Presentations and Separations: A Step-wedged Randomized Trial
Authors: Jennifer Mann , Thompson Fintan , McDermott Robyn , Esterman Adrian , Strivens Edward 
Published: March 2021

Abstract
BackgroundAn ageing population and rise in multi-morbidity increase hospital utilisation and acuity of presentation, particularly amongst the older person with complex needs. Health systems must reorient towards preventative and co-ordinated care to reduce hospital demand and achieve positive and fiscally responsible client outcomes. Integrated care models can improve outcomes for the older person by aligning primary practice with the specialist health care and social services required to manage complex needs. This paper describes the impact of a community facing program that integrates care at the primary-secondary interface on the rate of Emergency Department (ED) presentation and hospital separations amongst older people with complex needs.MethodsThe OPEN ARCH study is a multicentre randomised controlled trial with a stepped wedge cluster design. General practitioners (GPs; n=14) are considered ‘clusters’ each comprising a mixed number of participants. 80 community dwelling persons over 70 years of age if non-Indigenous and over 50 years of age if Indigenous were included in the study. Clusters were randomly assigned to the time at which they would commence the OPEN ARCH intervention, with intervention periods of 3, 6 and 9 months duration. Each participant was its own control. ED presentations and hospital separations were collected from Queensland Health Casemix data and analysed with multilevel mixed-effects Poisson regression modelling to determine the effectiveness of the OPEN ARCH intervention. Data were analysed at the cluster and participant levels.ResultsThe OPEN ARCH intervention was found to not make a statistically significant difference to ED presentations or hospitalisations. However, a stabilising of ED presentations and trend toward lower hospitalisation rates over time was observed. ConclusionsWhile this study detected no statistically significant different change in ED presentations or hospital separations, a plateauing of ED presentation and hospitalisation ratesis a clinically significant finding for older persons with complex needs. Multi-sectoral integrated programs of care require an adequate preparation period and sufficient duration of intervention for effectiveness to be measured. Trial registrationThe OPEN ARCH study received ethical approval from the Far North Queensland Human Research Ethic Committee, HREC/17/QCH/104 – 1174 and is registered on the Australian and New Zealand Trials Registry, ACTRN12617000198325p.
Enteric Methane Emission Estimates for Kenyan Cattle in a Nighttime Enclosure Using a Backward Lagrangian Stochastic Dispersion Technique
Authors: Wolz Kevin , Leitner Sonja , Merbold Lutz , Wolf Benjamin , Mauder Matthias 
Published: March 2021

Abstract
This study provides methane (CH4) emission estimates for mature female African beef cattle in a semi-arid region in Southern Kenya using open-path laser spectroscopy together with a backward Lagrangian Stochastic (bLS) dispersion modeling technique. We deployed two open-path lasers to determine 10-minute averages of line-integrated CH4 measurements upwind and downwind of fenced enclosures (so-called bomas: a location where the cattle are gathered at nighttime) during 14 nights in September/October 2019. The measurements were filtered for wind direction deviations and friction velocity before the model was applied. We compared the obtained emission factors (EFs) with the Intergovernmental Panel on Climate Change (IPCC) Tier 1 estimates for the Sub-Saharan African (SSA) countries, which were mostly derived from studies carried out in developed countries and adapted to the conditions in Africa. The resulting mean EF of 75.4 ± 5.69 kg yr-1 and the EFs calculated from other studies carried out in Africa differ considerably from each other, which indicates the need for the further development of region-specific EFs to improve the IPCC Tier 1 estimates.
Information Flow to Increase Support for Tidal Energy Development in Remote Islands of a Developing Country: Agent-Based Simulation of Information Flow in Flores Timur Regency, Indonesia
Authors: Ramachandran Rohit , Kularathna A. H. T. Shyam , Matsuda Hirotaka , Takagi Ken 
Published: March 2021

Abstract
BackgroundPublic awareness is crucial for successful deployment of tidal energy, a renewable energy source that can provide clean electricity to remote islands. However, considering public attitudes on tidal energy are not well known, especially in developing countries, a barrier exists in implementing public engagement strategies. This study aims to contribute by identifying strategies for information provision – the initial step in public engagement – and estimate how these can be engaged to enhance support for tidal energy among the local public in a remote area of a developing country, in this case, Flores Timur Regency, Indonesia, considering their socio-cultural background.MethodsIn this paper we employ statistical analyses using Multinomial Probit modelling to identify the key variables that shape information flow. The aptness of the variables is then verified using post-estimation techniques for their use as input parameters for simulation of the information-flow in the field study area. Agent-Based Simulation (ABS) is employed to replicate the actual conditions in Flores Timur regency, Indonesia and simulate the flow of information through the local community.ResultsAccording to the Multinomial Probit estimations, the people belonging to the top hierarchical group show a higher probability to support tidal energy compared to the members belonging to the lower groups. Understandably, it takes around twice as many information flow cycles to disseminate information to the members of the lowest hierarchical group, compared to the members of the top hierarchical group. Results also show that increasing the amount of available information has a positive impact on information dissemination.ConclusionsThis study found that information provision is highly effective with propagation of information that specifically highlights the individual benefits, rather than the community benefits of tidal energy. Additionally, savings in terms of cost, time, and effort can be realized if the most influential members of the local community are targeted initially before including all other stakeholders. The study also found that locals absorb more information and increase their support for tidal energy when additional data is made available. Finally, albeit long-term strategy, information provision becomes most effective when the local population gains higher educational capabilities.
Escherichia coli harboring mcr-1 on a Novel Plasmid in a Mink
Authors: Chang Weishan , Wang Xinxing , Peng Jun 
Published: March 2021

Abstract
Background: Antimicrobial resistant Escherichia coli from mink can be transmitted to human through water environment. Therefore, it is necessary to routinely detect the presence of antimicrobial resistance genes of mink. Methods: In the current study, we described the characterization of an mcr-1-positive Escherichia coli isolate from a dead mink in Dongping Lake, China. The molecular characterization of plasmids was also investigated. Results: The strain was resistant to colistin, but it remained susceptible to several other agents, including amikacin, piperacillin-tazobactam and all carbapenems. Escherichia coli Mink_1 belonged to sequence type 140. Whole genome sequencing(WGS) showed that two plasmid, pM1mcr and pM1ctx, coexisted in the same bacterial. Blast and phylogenetic tree imply that epidemic plasmids are vehicles for the dissemination of colistin resistance genes among the Enterobacterale. Conclusion: Restrictive and rational use of antibiotics in animal husbandry should be taken to reduce the dissemination of mcr-1.
Determinants of Diarrheal Diseases among Under Five Children in Jimma Geneti District, Oromia Region, Ethiopia, 2020: a case-control study
Authors: Mosisa Dejene , Aboma Mecha , Girma Teka , Shibru Abera 
Published: March 2021

Abstract
Background: Globally, in 2017, there are nearly 1.7 billion cases of childhood diarrheal diseases and it is the second most important cause of morbidity and mortality among under-five children in low-income countries including Ethiopia. Sanitary conditions, Poor housing, unhygienic environment, inadequate safe water supply, cohabitation with domestic animals that may carry human pathogens, and lack of storage facilities for food combining with socio-economic and behavioral factors are the common determinates of diarrhea diseases and had a large impact on diarrhea incidence in most of the developing countries Methods: A Community-based unmatched case-control study design was conducted on 407 systematically sampled under-five children of Jimma Geneti District (135 with diarrhea and 272 without diarrhea) from May 01 to 30, 2020. Data was collected using an interview administered questionnaire and observational checklist adapted from the WHO/UNICEF core questionnaire and other related literature. Descriptive, bivariate, and multivariate binary logistic regression analysis were done by using SPSS version 20.0 Result: Socio-demographic determinants such as being a child of 12-23 months age (AOR 3.3, 95% CI 1.68-6.46) and parents/legal guardian’s history of diarrheal diseases (AOR 7.38, 95% CI 3.12-17.44) were significantly associated with diarrheal diseases among under-five children. Environmental and Behavioral factors such as unavailability of handwashing facility nearby latrine (AOR 5.22, 95% CI 3.94-26.49), lack of hand-washing practice at critical times (AOR 10.6, 95% CI 3.74-29.81), improper domestic solid waste disposal practice (AOR 2.68, 95% CI 1.39-5.18) and not vaccinated against rotavirus (AOR 2.45, 95% CI 1.25-4.81) were found important determinants of diarrheal diseases among under-five children. Conclusion: Unavailability of hand-washing facility nearby latrine, parent’s/legal guardian’s history of last two weeks diarrheal diseases, improper latrine utilization, lack of hand-washing practice at critical times, improper solid waste disposal practices, and rotavirus vaccination status were the determinants of diarrheal diseases among under-five children identified in this study. Thus, promoting households through the provision of continuous and modified health information on the importance of sanitation, personal hygiene as well as vaccination against rotavirus, which is fundamental to decrease the burden of diarrheal disease among under-five children.
Influence of severity of total bile acids and mode of delivery on the perinatal outcomes in intrahepatic cholestasis of pregnancy
Authors: Kong Chengcai , Mei Fenglin , Xue Pingping , Cao Jingyun , Li Yong , Dong Yishan 
Published: March 2021

Abstract
To assess the associations between mode of delivery and pregnancy outcomes in patients with intrahepatic cholestasis of pregnancy (ICP) based on the severity of maternal hypercholanemia. A hospital-based retrospective cohort study was performed between January 1, 2015, and December 31, 2019. Among the 177 women with mild total bile acids, 123 (69.5%) had a vaginal delivery and 54 (30.5%) underwent a cesarean delivery, of which 42 (23.7%) were planned and 12 (6.8%) were unplanned. Among the 50 severe ICP women, 13 (26.0%) had a vaginal delivery and 37 (74.0%) underwent a cesarean delivery, of which 26 (52.0%) were planned and 11 (22.0%) were unplanned. Severe ICP was associated with an increased risk of preterm delivery (P < 0.001), low birthweight (P=0.001), and neonatal intensive care unit admission (P < 0.001). Women with severe ICP (aOR, 5.017; 95% CI 1.848–13.622), planned cesarean delivery (aOR, 5.444; 95%CI 1.723–17.203), or unplanned cesarean delivery (aOR, 5.792; 95%CI 1.384–24.236) had increased risks of adverse fetal outcomes compared to controls. Both planned and unplanned cesarean delivery are associated with a higher incidence of adverse fetal outcomes and severe ICP than vaginal delivery.
Functional and anatomical characterization of corticotropin-releasing factor receptor subtypes of the rat spinal cord involved in somatic pain relief
Authors: Mousa Shaaban A. , Shaqura Mohammed , Khalefa Baled , Li Li , Al-madol Mohammed , Treskatsch Sascha , Schäfer Michael 
Published: March 2021

Abstract
Background: Corticotropin-releasing factor (CRF) orchestrates our body’s response to stressful stimuli. Pain is often stressful and counterbalanced by activation of CRF receptors along the nociceptive pathway, although the involvement of the CRF receptors of subtypes 1 and/or 2 (CRF-R1 and CRF-R2, respectively) in CRF-induced analgesia remains controversial.Methods: This study aimed to examine CRF-R1 and CRF-R2 expression within spinal cord of rats with Freund’s complete adjuvant-induced hindpaw inflammation using reverse transcriptase polymerase chain reaction, Western blot, radioligand binding, and immunofluorescence confocal analysis, Western blot, immunohistochemistry, and radioligand binding. Moreover, paw pressure algesiometry examined antinociceptive effects of intrathecal (i.t.) CRF and their possible antagonism through CRF-R1 and/or CRF-R2 selective antagonists as well as opioid receptor antagonist naloxone.Results: Our results demonstrated mainly CRF-R2 mRNA, protein, binding sites and immunoreactivity in dorsal horn of rat spinal cord. In parallel, i.t. low, systemically inactive doses of CRF as well as CRF-R2 agonists elicited potent antinociceptive effects which are dose-dependent and antagonized exclusively by i.t. CRF-R2 (K41498), but not CRF-R1 (NBI35965) antagonist. Moreover, i.t. CRF elicited inhibition of somatic pain that was dose-dependently reversed by the opioid antagonist naloxone. Consistently, double immunofluorescence confocal microscopy showed CRF-R2 on enkephalin (ENK) containing inhibitory interneurons in close opposition of incoming, mu-opioid receptor-immunoreactive nociceptive neurons but not on pre- nor on postsynaptic sensory neurons of the spinal cord.Conclusion: Taken together, these findings suggest that i.t. CRF or CRF-R2 agonist inhibits inflammatory somatic pain which occurs most predominantly through CRF-R2 receptors located on spinal enkephalinergic inhibitory interneurons resulting in endogenous opioid-mediated pain inhibition.
Lycopene Supresses Palmitic acid-induced Neuro-oxidoinflammation via Attenuation of Oxidative stress and NF-κB-p65 activation in Female Rats
Authors: Ugbaja Regina Ngozi , James Adewale Segun , Ugwor Emmanuel Ifeanyichukwu , Akamo Adio Jamiu , Thomas Funmilola Clara , Kosoko Ayokulehin Muse 
Published: March 2021

Abstract
Neuroinflammation can be triggered by certain nutrients. Effect of lycopene against palmitic acid-induced neuroinflammation in female rats has not been explored. This study evaluated the effects of lycopene against palmitic acid (PA)-induced neuroinflammation in rats. Thirty rats (weighing 150–200 g) were randomised into six groups (n = 5): Normal control, PA control, PA + lycopene (0.24 mg/kg), PA + lycopene (0.48 mg/kg), lycopene (0.24 mg/kg), and lycopene (0.48 mg/kg), respectively. After seven weeks of PA challenge including two weeks of lycopene treatment, brain was excised for analyses. The PA-induced significantly (p < 0.05) increased adenosine deaminase, monoamine oxidase-A, nucleotides tri-phosphatase, 5’-nucleotidasea, acetylcholine esterase, myeloperoxidase activities, and malondialdehyde level, reduced significantly post-treatment. Conversely, catalase and glutathione peroxidase activities, and reduced glutathione levels decreased (PA control) by 43%, 34%, and 12%, respectively, compared with the Control. Also, PA triggered a decrease in the brain phospholipids (11.43%) and cholesterol (11.11%) levels, but increased triacylglycerol level (50%). Furthermore, upregulated expressions of IL-1β, IL-6, and NF-ĸB-p65 in the PA control were attenuated, while decreased IL-10 was upregulated after treatment. Severe vacuolation in PA control was normalized by lycopene. This study concludes that, lycopeneameliorated PA-induced neuroinflammation, probably via attenuation of oxidative stress, and downregulation of TLR4/ NF-κB -p65 axis.
Nasal HFOV Versus Nasal IPPV as a Post Extubation Respiratory Support in Preterm Infants - a Randomised Controlled Trial
Authors: Seth Soutrik , Saha Bijan , Saha Anindya Kumar , Mukherjee Suchandra , Hazra Avijit 
Published: March 2021

Abstract
Early and successful extubation prevents several morbidities in preterm newborns. Several secondary noninvasive respiratory modalities exists but with their merits and demerits. Given the superior respiratory efficacy of nasal high frequency oscillatory ventilation (nHFOV), we tried to examine whether nHFOV could reduce reintubation rates compared to nasal intermittent positive pressure ventilation (NIPPV) during the postextubation phase in preterm infants. Stratified randomisation based on gestational age was done for 86 mechanically ventilated preterm infants between 26 - 37 weeks of gestation within 2 weeks of age, to receive either nHFOV or NIPPV post extubation. The main objective was to compare extubation failure within 72 hours following extubation and secondarily feed intolerance, necrotising enterocolitis (NEC) (> stage 2), hemodynamically significant patent ductus arteriosus (hsPDA), intraventricular haemorrhage (IVH) (> grade 3), retinopathy of prematurity (ROP), bronchopulmonary dysplasia (BPD), death, hospital stay duration. No statistical difference was noted for primary outcome ( RR 0.8, 95% CI: 0.23 to 2.78; p = 1.00 ) and secondary outcomes . However nHFOV appeared superior in respect to feed tolerance rates, pCO2 washout and hospital stay duration. Conclusion: Extubation failure within 72 hours in infants less than 37 weeks of gestation did not differ between the two groups. However nHFOV seems promising in reducing enteral feeding issues and hospital stay duration . Larger multicentre studies are required for exploring benefits of nHFOV. Trial registration: www.ctri.nic.in id CTRI/2019/07/020055, registration date July 5, 2019