bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mixed Origins: HIV gp120-specific memory develops from pre-existing memory and naïve B cells following vaccination in humans
Authors: Basu Madhubanti , Piepenbrink Michael S. , Fucile Christopher , Bunce Catherine A. , Man Li-Xing , Liesveld Jane , Rosenberg Alexander F. , Keefer Michael C. , Kobie James J. 
Published: September 2021

AbstractThe most potent and broad HIV envelope (Env)-specific antibodies often when reverted to their inferred germline versions representing the naïve B cell receptor, fail to bind Env suggesting that the initial responding B cell population is not exclusively comprised of a naïve population, but also a pre-existing cross-reactive antigen-experienced B cell pool that expands following Env exposure. Previously we isolated gp120-reactive monoclonal antibodies (mAbs) from participants in HVTN 105, an HIV vaccine trial. Using deep sequencing VH-lineage tracking we identified several of these mAb lineages in pre-immune peripheral blood. Several of these pre-immune lineages also persisted in the bone marrow, including CD138+ long-lived plasma cell compartment, ∼7 months after the final vaccination. The majority of the pre-immune lineage members included IgM, however IgG and IgA members were also prevalent and exhibited somatic hypermutation. These results suggest that vaccine-induced gp120-specific antibody lineages originate from both naïve and cross-reactive memory B cells.
Natural variation in Arabidopsis ISR1 affects iron localization and induced systemic resistance
Authors: Socha Amanda L. , Song Yi , Ross Brandon S. , Bush Jenifer , Ausubel Frederick M. , Guerinot Mary Lou , Haney Cara H. 
Published: September 2021

AbstractBeneficial root-associated bacteria can induce systemic resistance (ISR) to foliar pathogens and there is known transcriptional and genetic overlap in the root response to iron deficiency and ISR. A previous study found that there is natural variation in ISR among Arabidopsis accessions. The Ws accession is deficient in ISR, and the responsible recessive genetic locus, named ISR1, was mapped to chromosome 3. To find candidate genes that may underlie ISR deficiency in Ws, we identified genes that are induced in response to the ISR-triggering bacterium Pseudomonas simiae WCS417 and to iron stress and that have non-synonymous mutations in the Ws genome with respect to the ISR-responsive Col-0. We identified a kelch-domain containing protein encoded by At3g07720 that has a genomic rearrangement in Ws. We found that overexpression of Col-0 At3g07720 restores ISR to Ws, indicating that At3g07720 encodes ISR1. Isr1 loss of function mutants do not affect plant growth under iron limiting conditions but have increased levels of apoplastic iron. We found that iron supplementation, P. simiae WCS417, or a loss of isr1 enhance ROS production in a non-additive manner, suggesting they work through the same mechanism to enhance resistance. Our findings show that ISR1 is required for iron localization, immunity, and ISR, and suggest that increased iron uptake induced by ISR-eliciting bacteria may directly contribute to immunity through increased reactive oxygen production.
Host genomic influence on bacterial composition in the switchgrass rhizosphere
Authors: Sutherland Jeremy , Bell Terrence , Trexler Ryan V. , Carlson John E. , Lasky Jesse R. 
Published: September 2021

AbstractHost genetic variation can shape the diversity and composition of associated microbiomes, which may reciprocally influence host traits and performance. While the genetic basis of phenotypic diversity of plant populations in nature has been studied, comparatively little research has investigated the genetics of host effects on their associated microbiomes. Switchgrass (Panicum virgatum) is a highly outcrossing, perennial, grass species with substantial locally adaptive diversity across its native North American range. Here, we compared 383 switchgrass accessions in a common garden to determine the host genotypic influence on rhizosphere bacterial composition. We hypothesized that the composition and diversity of rhizosphere bacterial assemblages would differentiate due to genotypic differences between hosts (potentially due to root phenotypes and associated life history variation). We observed higher alpha diversity of bacteria associated with upland ecotypes and tetraploids, compared to lowland ecotypes and octoploids, respectively. Alpha diversity correlated negatively with flowering time and plant height, indicating that bacterial composition varies along switchgrass life history axes. Narrow-sense heritability (h2) of the relative abundance of twenty-one core bacterial families was observed. Overall compositional differences among tetraploids, due to genetic variation, supports wide-spread genotypic influence on the rhizosphere microbiome. Lastly, a genome-wide association study identified 1,861 single-nucleotide polymorphisms associated with 110 families and genes containing them related to potential regulatory functions. Our findings suggest that switchgrass genomic and life-history variation influences bacterial composition in the rhizosphere, potentially due to host adaptation to local environments.
Methodological guidelines for isolation and purification of plant extracellular vesicles
Authors: Huang Yifan , Wang Shumei , Cai Qiang , Jin Hailing 
Published: September 2021

ABSTRACTPlant extracellular vesicles (EVs) have become the focus of rising interest due to their important roles in the cross-kingdom trafficking of molecules from hosts to interacting microbes to modulate pathogen virulence. However, the isolation of pure intact EVs from plants still represents a considerable challenge. Currently, plant EVs have been isolated from apoplastic washing fluid (AWF) using a variety of methods. Here, we compare two published methods used for isolating plant EVs, and provide a detailed recommended method for AWF collection from Arabidopsis thaliana, followed by EV isolation via differential ultracentrifugation. To further separate and purify specific subclasses of EV from heterogeneous vesicles, sucrose or iodixanol density-based separation and immunoaffinity capture are then utilized. We found that immunoaffinity capture provides a significant advantage for specific EV isolation when suitable specific EV biomarkers and their corresponding antibodies are available. Overall, this study guides the selection and optimization of EV isolation methods for desired downstream applications.
Too much is not always good: the inverted U-shaped link between green customer integration and opportunistic behavior
Authors: Shi Haiqing , Feng Taiwen , Li Zhiyi 
Published: September 2021

PurposeThe purpose of this study is to explore the inverted U-shaped relationship between green customer integration (GCI) and opportunistic behavior, as well as the moderating effects of contractual control and relational norms.Design/methodology/approachThe authors conducted hierarchical regression analysis using two-waved data from 206 Chinese manufacturing firms to test hypotheses.FindingsThe authors found that GCI has an inverted U-shaped effect on opportunistic behavior. Furthermore, both contractual control and relational norms negatively moderate the inverted U-shaped relationship between GCI and opportunistic behavior.Originality/valueThis study uncovers an inverted U-shaped link between GCI and opportunistic behavior by combining transaction cost economics and social exchange theory. Furthermore, this study reveals contractual control and relational norms can be deemed as two boundary conditions affecting the inverted U-shaped GCI–opportunistic behavior relationship. This study also offers managerial implications for firms curbing opportunistic behavior that may result from GCI.
Exploring the interrelationship and roles of employee–organization relationship outcomes between symmetrical internal communication and employee job engagement
Authors: Lee Ejae , Kang Minjeong , Kim Young , Yang Sung-Un 
Published: September 2021

PurposeThis paper aims to investigate how employee–organization relationship (EOR) outcomes – types and qualities – are interrelated and how employees' perceptions of types (exchange and communal EORs) and qualities (trust, satisfaction, commitment, and control mutuality) play a role in their evaluations of symmetrical internal communication (SIC) and employee job engagement (EJE).Design/methodology/approachThis study conducted an online survey of full-time employees (N = 804) from major US industries. This study performed a confirmatory factor analysis to check the validity and reliability of the measurement model using latent variables and then conducted structural equation modeling.FindingsThe findings demonstrate that employees' perceptions of both exchange and communal EORs are associated with each of the four EOR qualities. The results also show that only communal EORs have a significant relationship with perceived SIC and that employees' perceptions about one of the EOR quality indicator, satisfaction with an organization, has a significant association with their perceived EJE.Originality/valueThis study contributes to relationship management theory within the internal context by examining the interrelationship between each of the EOR types and qualities that are perceived by employees. This paper also suggests the practical importance of developing not only communal but also exchange EORs to enhance EOR quality. Additionally, the results imply that SIC programs could help to enhance employees' perceptions of communal EORs and employees could be engaged in their workplace when they are satisfied with their organizations.
Study of isolation effectiveness of nuclear reactor building with three-dimensional seismic base isolation
Authors: Zhu Xiuyun , Pan Rong , Li Jianbo , Lin Gao 
Published: September 2021

PurposeIn recent years, three-dimensional (3D) seismic base isolation system has been studied extensively. This paper aims to propose a new 3D combined isolation bearing (3D-CIB) to mitigate the seismic response in both the horizontal and vertical directions.Design/methodology/approachThe new 3D-CIB composed of laminated rubber bearing coupled with combined disk spring bearing (CDSB) was proposed. Comprehensive analysis of constitution and theoretical derivation for 3D-CIB were presented. The advantage of CDSB is that the constitution can be flexibly adjusted according to the requirements of the bearing capacity and vertical stiffness. Hence, four different combinations of CDSB were designed for the 3D-CIB and employed to isolate nuclear reactor building. A comparative study of the seismic response in terms of seismic action, acceleration floor response spectra (FRS), peak acceleration and relative displacement response was carried out.Findings3D-CIB can effectively reduce seismic action, FRS and peak acceleration response of the superstructure in both the horizontal and vertical directions. Overall, the horizontal isolation effectiveness of 3D-CIB was slightly influenced by vertical stiffness. The decrease in the vertical stiffness of the 3D-CIB can reduce the vertical FRS and shift the peak values to a lower frequency. The vertical peak acceleration decreased with a decrease in the vertical stiffness. The superstructure exhibited a rocking effect during the earthquake, and the decrease in the vertical stiffness may increase the rocking of the superstructure.Originality/valueAlthough the advantage of 3D-CIB is that the vertical stiffness can be flexibly adjusted by different constitutions, the vertical stiffness should be designed by properly accounting for the balance between the isolation effectiveness and displacement response. This study of isolation effectiveness can provide the technical basis for the application of 3D-CIB into real engineering of nuclear power plants.
Integrating lean production strategies, virtual reality technique and building information modeling method for mass customization in cabinet manufacturing
Authors: Zhang Yuxuan , Wang Jingwen , Ahmad Rafiq , Li Xinming 
Published: September 2021

PurposeIn response to increasing demand for a fully customized and individualized home environment, mass customization (MC) has been suggested as an effective strategy to fulfill the customer’s customization needs while keeping production cost-effectiveness. However, in current practice, the implementation of the MC in the industrialized housing industry has not achieved an ideal level. Little effort was devoted to customer value generation and achieving lean production in a multi-disciplinary MC environment. In this concern, a highly efficient and flexible production information system is expected to capture accurately the customer’s demand and efficiently perform work planning for encouraging customer involvement and mass efficiency production.Design/methodology/approachTo gain an insight into the development of the MC production information system for the housing industry and to depict the interaction among system modules, this study used a design science research methodology for a case study of customized cabinet production information system development.FindingsA prototype of the production information system was proposed in this paper, supported by three information technologies to facilitate the MC implementation in the millwork manufacturer. A focus group discussion method was carried out for evaluating the system feasibility and the subsequent survey analysis on the virtual reality (VR) interface experiment. The evaluation process results showed that the VR interface is an effective medium for design information communication and encourages customer involvement. Most participants believed that the proposed production information system could generally benefit the MC implementation and improve production efficiency.Originality/valueThis study integrated lean production principles along with building information modelling, VR and discrete-event simulation in the production information system to assist the manufacturer in effectively handling variant product information and enabling quicker reactions in response to diverse customer requirements in housing industries. The coordination among system modules and the managed information flow could be a valuable reference for future MC production system development in housing industries.
The positive experiences associated with coming out at work
Authors: Salter Nicholas P. , Sasso Thomas 
Published: September 2021

PurposeMuch research has focused on the negative aspects of disclosing sexual orientation and/or gender identity in the workplace but less has explicitly examined the positive aspects. This lack of research is problematic as this can oversimplify the work lives of lesbian, gay, bisexual, transgender or queer (LGBTQ) people. The current study examines positive intrapersonal, interpersonal and work opportunity experiences associated with coming out in the workplace as LGBTQ.Design/methodology/approachThe current study surveyed 135 working adults who identified as LGBTQ and used a mixed qualitative and quantitative design to examine the relationship between disclosure and various positive workplace experiences.FindingsResults suggest that sexual orientation disclosure at work was related to participants perceiving multiple positive interpersonal as well as work opportunity experiences. Furthermore, results suggest gender identity disclosure was similar to, but not the same as, sexual orientation disclosure in terms of perception of positive experiences.Originality/valuePrevious research on disclosure at work has taken a somewhat narrow and typically quantitative approach. The current study provides more nuance to the phenomenon by broadly examining multiple positive experiences associated with disclosure and studying them qualitatively in order to best understand participants' experiences in their own voices.
Emerging partnerships between non-profit organizations and companies in reverse supply chains: enabling valorization of post-use textile
Authors: Zhuravleva Anna , Aminoff Anna 
Published: September 2021

PurposeThe European Union (EU) member states are obligated to implement the separate collection of textile waste by the year 2025. Nowadays, non-profit organizations (NPOs) are the largest collectors of post-use textiles. In support of upcoming changes, this study develops an understanding of barriers and drivers for establishing partnerships between NPOs and companies in reverse textile supply chains.Design/methodology/approachThis study adopts the embedded single-case design. The main data source is semi-structured interviews with NPOs, companies and research institutes in Finland, identified through intensity case sampling. The drivers and barriers are categorized into seven categories: environmental, economic, social, institutional, technological and informational, supply chain and organizational categories.FindingsThis study elaborates on the barriers and drivers in a new context of textile valorization and prioritizes them. The study identifies the alignment of interests and goals, increased transparency and clarity of terminology and other main factors in establishing the partnership.Research limitations/implicationsExpanding the geographical boundaries of current research will capture the experiences of NPOs and companies in other contextual settings.Practical implicationsThis study contributes to the existing knowledge with a broad picture of different barriers and drivers. The findings intend to support the integration of NPOs in reverse textile supply chains.Social implicationsThe partnership can potentially minimize the export of post-use textiles to developing countries, thus reducing the negative environmental footprint and social impact of the textile industry.Originality/valueThe study looks at an emerging form of partnership between NPOs and companies in reverse supply chains for enabling valorization of post-use textiles.
Application of blockchain information technology in Ṣukūk trade
Authors: Busari Saheed Abdullahi , Aminu Sikiru Olanrewaju 
Published: September 2021

Purpose
This study aims to explore the opportunities and challenges in activating a Smart Contract to enhance the efficiency and effectiveness of Ṣukūk offerings in the Islamic capital market.

Design/methodology/approach
The study adopts a mono-method qualitative approach. Data were obtained from survey interviews of two issuances on the fusion of smart contracts in Ṣukūk structures that were Sharīʿah-compliant. A thematic approach was further used to analyze the interview data based on the onion research method while opportunities and challenges of activating the Smart Ṣukūk (SṢ) relied on doctrinal evidence.

Findings
The results from the issuances across two jurisdictions showed that deployment of SṢ can resolve contractual ambiguities arising from Sharīʿah interpretations, jurisdictional policies and legal regime issues, which affect Ṣukūk origination and issuances especially on the right of investors in the event of Ṣukūk defaults. Although SṢ is automated, the third party’s presence is not eliminated as the blockchain platform still relies on the validators who are usually blockchain developers functioning as a third party in the Ṣukūk chain.

Research limitations/implications
The study relies on doctrinal literature to explain the features and requirements of SṢ. The empirical approach is limited to interview data based on local SṢ issuances. Future studies need to explore regulators’ role and global standards in cross-border issuance of SṢ with multiple jurisdictions/laws.

Practical implications
The paper concludes that the offering of SṢ using local currency has been successful in the two issuances because of the facilitative regulatory environment. However, addressing Ṣukūk’s challenges in cross-border offerings would require guidance from international standard-setters such as the Accounting and Auditing Organization for Islamic Financial Institutions and the Islamic Financial Services Board.

Originality/value
This study is an advanced application of smart contracts to alleviate the related Ṣukūk challenges in the Islamic capital market.
Consumer ethnocentrism and purchasing behavior: moderating effect of demographics
Authors: Akbarov Shahin 
Published: September 2021

Purpose
This study aims to investigate consumer ethnocentrism and determine its impact on actual purchasing behavior in relation to six product categories. It also examines the role of demographic variables as moderators in the relationship between ethnocentric tendencies and purchasing behavior.

Design/methodology/approach
A total of 467 completed questionnaires were obtained through convenience and snowball sampling. Data were analyzed using SPSS-24 and AMOS-23 software: SPSS to conduct the exploratory factor analysis and AMOS for the confirmatory factor analysis. Regression analysis was performed to determine the effect of ethnocentrism on purchasing behaviors; the SPSS process was used to test the moderating effects.

Findings
The consumer ethnocentric tendencies scale was collected in two dimensions: hard ethnocentrism, which influences actual purchasing behavior in five product categories; and soft ethnocentrism, which influences actual purchasing behavior in only two product categories. The results show that the effect of consumer ethnocentrism on purchasing behavior differs across product categories. Further, gender, marital status and personal income moderate this relationship.

Originality/value
Few studies investigate purchasing behavior across several product categories and the literature on consumer ethnocentrism does not address the impact of demographic variables as moderators in consumer behavior. This study contributes to the existing literature in four ways. First, it was conducted in Azerbaijan, a country with specific characteristics. Second, it examines the impact of ethnocentric tendencies on actual purchasing behavior. Third, it examines purchasing behavior in relation to six different product categories. Fourth, the moderating effect of demographic variables was tested.
The requirements for maintenance management systems (MMS) at Malaysian polytechnic: a case study
Authors: Ismail Zul-Atfi 
Published: September 2021

PurposeThe conventional methods practiced by Development and Facilities Management Unit (UPPF) faced issues due to management deficiencies and negligent staffs to handle operation and maintenance processes of Chemical Facility (CFs) management at Malaysian Polytechnic. The prime objective of this paper is to improve the conventional methods which tend to be both cumbersome and ineffective in the Maintenance Management Systems (MMS) for UPPF at Malaysian Polytechnic.Design/methodology/approachPrimary data was gathered through the interview to develop the proposed system. The major conclusion drawn from the interview results was that there is a lack of comprehensive MMS that integrate operation and maintenance processes of CF management and software programming that provide the guides for decision-making processes. The interview results also revealed irregularities within the Malaysian Polytechnic's maintenance management database.FindingsThe conventional methods had significantly little emphasis on defect diagnosis tools. It had also increased the inadequate strategic decision making to analyse information in improving the maintenance project outcomes for Malaysian Polytechnic's building. New MMS tools is suggested from findings and lessons learned as a good practice to reduce the frequent overhauling and repairs on the durability's performance and design of building control instrument.Originality/valueThe new MMS potentially transform operation and maintenance processes of CF management into one of the most sophisticated technologies by providing access to all information published by all Malaysian Polytechnics institutions. This is in order to foster financial cooperation, the idea that being that Polytechnics who compete with one another become financially independent towards successful achievements of CF management for laboratory building and equipment.