bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of electroacupuncture treatment in the prevention of negative moods in healthy young men after 30 h of total sleep deprivation: study protocol for a single-center, single-blind, parallel-arm, randomized clinical trial
Authors: Yan Bing , Wang Fu-chun , Ma Tian-shu , Liu Yan-ze , Liu Wu , Cheng Lei , Wang Zi-yuan , Wang Zhong-ke , Liu Cheng-yu 
Published: November 2021

Abstract
Background
Sleep deprivation (SD) among young adults is a major public health concern. In humans, it has adverse effects on mood and results in serious health problems. Faced with SD, persons may take precautionary measures to try and reduce their risk. The aim of this study is to evaluate the efficacy and safety of electroacupuncture (EA) for the prevention of negative moods after SD. In addition, we will do a comparison of the effects of EA on mood after SD at different time points.

Methods
This randomized controlled trial (RCT) will be performed at the First Affiliated Hospital of Changchun University of Chinese Medicine in China. The Standards for Reporting Interventions in Clinical Trials of Acupuncture 2010 will be strictly adhered to. Forty-two healthy male volunteers will be distributed into acupoints electroacupuncture (AE) group, non-acupoints electroacupuncture (NAE) control group, or blank control group. This trial will comprise 1-week baseline (baseline sleep), 1-week preventative treatment, 30-h total sleep deprivation (TSD), and 24-h after waking follow-up period. Participants in the AE group and the NAE control group during the preventative treatment period will be administered with EA treatment once daily for 1 week. Participants in the blank control group will not be administered with any treatment. The primary outcome will be the Profile of Mood States (POMS) Scale. Secondary outcome measures will include changes in the Noldus FaceReader (a tool for automatic analysis of facial expressions) and Positive and Negative Affect Schedule (PANAS) Scale. Total sleep deprivation will be 30 h. During the 30-h TSD period, participants will be subjected to 11 sessions of assessment. Adverse events will be recorded.

Discussion
This study is designed to evaluate the efficacy and safety of EA for the prevention of negative moods after SD. The results of this trial will allow us to compare the effects of EA on mood after SD at different time points. Moreover, the findings from this trial will be published in peer-reviewed journals.

Trial registration
Chinese Clinical Trial Registry Chi2000039713. Registered on 06 November 2020
The Wikipedia Diversity Observatory: helping communities to bridge content gaps through interactive interfaces
Authors: Miquel-Ribé Marc , Laniado David 
Published: November 2021

AbstractIn this paper, we present the Wikipedia Diversity Observatory, a project aimed to increase diversity within Wikipedia content. The project provides dashboards with visualizations and tools which show content gaps in terms of imbalances in the coverage of topics, and of concepts that are not shared across Wikipedia language editions. The dashboards are built on datasets generated for each of the more than 300 existing language editions, with features that label each article according to geography, gender and other categories relevant to overall content diversity. Through various examples, we show how the tools encourage and help editors to bridge the gaps in Wikipedia content. Finally, we discuss the project’s impact on the communities and implications for the Wikimedia movement in a moment in which covering diversity is considered strategic.
Prognostic models of diabetic microvascular complications: a systematic review and meta-analysis
Authors: Saputro Sigit Ari , Pattanaprateep Oraluck , Pattanateepapon Anuchate , Karmacharya Swekshya , Thakkinstian Ammarin 
Published: November 2021

Abstract
Background
Many prognostic models of diabetic microvascular complications have been developed, but their performances still varies. Therefore, we conducted a systematic review and meta-analysis to summarise the performances of the existing models.

Methods
Prognostic models of diabetic microvascular complications were retrieved from PubMed and Scopus up to 31 December 2020. Studies were selected, if they developed or internally/externally validated models of any microvascular complication in type 2 diabetes (T2D).

Results
In total, 71 studies were eligible, of which 32, 30 and 18 studies initially developed prognostic model for diabetic retinopathy (DR), chronic kidney disease (CKD) and end stage renal disease (ESRD) with the number of derived equations of 84, 96 and 51, respectively. Most models were derived-phases, some were internal and external validations. Common predictors were age, sex, HbA1c, diabetic duration, SBP and BMI. Traditional statistical models (i.e. Cox and logit regression) were mostly applied, otherwise machine learning. In cohorts, the discriminative performance in derived-logit was pooled with C statistics of 0.82 (0.73‑0.92) for DR and 0.78 (0.74‑0.83) for CKD. Pooled Cox regression yielded 0.75 (0.74‑0.77), 0.78 (0.74‑0.82) and 0.87 (0.84‑0.89) for DR, CKD and ESRD, respectively. External validation performances were sufficiently pooled with 0.81 (0.78‑0.83), 0.75 (0.67‑0.84) and 0.87 (0.85‑0.88) for DR, CKD and ESRD, respectively.

Conclusions
Several prognostic models were developed, but less were externally validated. A few studies derived the models by using appropriate methods and were satisfactory reported. More external validations and impact analyses are required before applying these models in clinical practice.

Systematic review registration
PROSPERO CRD42018105287
The effect of motivation on employee engagement in public sectors: in the case of North Wollo zone
Authors: Engidaw Abriham Ebabu 
Published: November 2021

AbstractThe purpose of this study was to examine the effect of intrinsic and extrinsic motivation on employee engagement in public sectors. To meet this purpose, a quantitative and qualitative study survey was conducted and the researcher used an explanatory survey research design. The study was used both primary and secondary sources of data. The target population for this study was selected four city administrative office employees including all kebeles office employees and as a sample size, 349 questionnaires were filled and collected from these employees. The study used proportionately stratified with simple random sampling because to collect the relevant data and to give equal chance for all employees. Later on, statistical package for social science (SPSS) was used to the required test of inferential statistics; including reliability analysis, bivariate correlation, analysis of variance (ANOVA) test, and multiple regression analysis. The finding of the study revealed that extrinsic motivation was considered more significant than intrinsic and there was a positive significant relationship between intrinsic and extrinsic motivations with employee engagement. From the results, it was also concluded that the relationship and effect of extrinsic motivation were stronger on employee engagement as compared to intrinsic motivation. As a recommendation, the organizations should find which ways are able to motivate employees, what drives them, then making sure they utilize appropriate motivational techniques with each employee. Managers should also endeavor to communicate the organization’s purpose to employees. Finally, the organizations should take steps to incorporate the vision of employees for the organization with its own vision.
Plekhh1, a partner of myosin 1 and an effector of EphB2, controls the cortical actin network during cell repulsion
Authors: Prospéri Marie-Thérèse , Pernier Julien , Lachuer Hugo , Coudrier Evelyne 
Published: November 2021

ABSTRACT
EphB2–ephrinB signalling, which plays a major role in cell segregation during embryonic development and tissue homeostasis, induces an important reorganization of the cortical actin network. We have previously reported that myosin 1b contributes to reorganization of the cortical actin network upon EphB2 signalling. In this report, we identify Plekhh1 as a new partner of members of the myosin 1 family and EphB2 receptors. Plekhh1 interacts with myosin 1b via its N-terminal domain and with EphB2 via its C-terminal domain. Furthermore, Plekhh1 is tyrosine phosphorylated, and this depends on EphB2 kinase activity. Similar to the effects of manipulating levels of myosin 1b and myosin 1c, manipulation of Plekhh1 expression levels alters the formation of filopodia, the length of focal adhesions and the formation of blebs. Furthermore, binding of the Plekhh1 interacting domain to myosin 1b increases the motor activity of myosin 1b in vitro. Taken together, our data show that Plekhh1 is an effector of EphB2 and suggest that Plekhh1 regulates the cortical actin network via the interaction of its N-terminal domain with myosin 1 upon EphB2–ephrinB signalling.
Interobserver variability in target volume delineation for CT/MRI simulation and MRI-guided adaptive radiotherapy in rectal cancer
Authors: White Ingrid , Hunt Arabella , Bird Thomas , Settatree Sarah , Soliman Heba , Mcquaid Dualta , Dearnaley David , Lalondrelle Susan , Bhide Shree 
Published: November 2021

Objectives: Quantify target volume delineation uncertainty for CT/MRI simulation and MRI-guided adaptive radiotherapy in rectal cancer. Define optimal imaging sequences for target delineation. Methods: Six experienced radiation oncologists delineated clinical target volumes (CTVs) on CT and 2D and 3D-MRI in three patients with rectal cancer, using consensus contouring guidelines. Tumour GTV (GTVp) was also contoured on MRI acquired week 0 and 3 of radiotherapy. A STAPLE contour was created and volume and interobserver variability metrics were analysed. Results: There were statistically significant differences in volume between observers for CT and 2D-MRI-defined CTVs (p < 0.05). There was no significant difference between observers on 3D-MRI. Significant differences in volume were seen between observers for both 2D and 3D-MRI-defined GTVp at weeks 0 and 3 (p < 0.05). Good interobserver agreement (IOA) was seen for CTVs delineated on all imaging modalities with best IOA on 3D-MRI; median Conformity index (CI) 0.74 for CT, 0.75 for 2D-MRI and 0.77 for 3D-MRI. IOA of MRI-defined GTVp week 0 was better compared to CT; CI 0.58 for CT, 0.62 for 2D-MRI and 0.7 for 3D-MRI. MRI-defined GTVp IOA week three was worse compared to week 0. Conclusion: Delineation on MRI results in smaller volumes and better IOA week 0 compared to CT. 3D-MRI provides the best IOA in CTV and GTVp. MRI-defined GTVp on images acquired week 3 showed worse IOA compared to week 0. This highlights the need for consensus guidelines in GTVp delineation on MRI during treatment course in the context of dose escalation MRI-guided rectal boost studies. Advances in knowledge: Optimal MRI sequences for CT/MRI simulation and MRI-guided adaptive radiotherapy in rectal cancer have been defined.
The impact of vitamin D supplementation on peripheral neuropathy in a sample of Egyptian prediabetic individuals
Authors: Reda Halawa Mohamed , Zaky Ahmed Iman , Fawzy Abouelezz Nahla , Roushdy Mohamed Nagwa , Hany Abdelaziz Khalil Naira , Mahmoud Ali Hendawy Laila 
Published: November 2021

Background: Vitamin D deficiency is seen more frequently in diabetic patients with distal symmetrical polyneuropathy. Unfortunately, there is a shortage of data concerning prediabetic individuals with peripheral neuropathy (PN). Therefore, we aimed to study the association of vitamin D deficiency with PN severity and to determine the effect of vitamin D supplementation on PN in prediabetics. Methods: A case-control study was conducted consisting of 89 prediabetic individuals with PN and a control group of prediabetics without PN, recruited from the outpatient department of the National Institute of Diabetes and Endocrinology, Cairo, Egypt. All patients were screened for PN using clinical examination and Douleur Neuropathique 4 diagnostic questionnaire (DN4). Group A (with PN) was assessed for neuropathic severity using the Short-Form McGill Pain Questionnaire (SF-MPQ). In addition, 25-hydroxyvitamin D, ionized calcium, phosphorus, parathyroid hormone (PTH), glycated hemoglobin (HbA1c), fasting blood glucose (FBG), 2-hour post 75g glucose (2h-PPBG) and lipid profile were measured for both groups. Prediabetic patients with PN were given vitamin D3 200.000 IU IM monthly for three months. After three months, clinical assessment, DN4, SF-MPQ and all laboratory measures were repeated. Results: Vitamin D was not associated with the severity of PN patients. However, supplementation of vitamin D resulted in a highly significant improvement in glycemic parameters , p≤0.001. Interestingly, neuropathy score and severity before vitamin D supplementation were (6.4±1.6 and 28.3±7.2) and after became (2.5±0.9 and 17±6.3, p≤0.001). Conclusion: Correction of vitamin D deficiency in prediabetics with PN as well as hypovitaminosis D, improves glycemic parameters, PN score and severity.
Edge computing for Vehicle to Everything: a short review
Authors: Abdullah Mohd. Fikri Azli , Yogarayan Sumendra , Abdul Razak Siti Fatimah , Azman Afizan , Muhamad Amin Anang Hudaya , Salleh Mazrah 
Published: November 2021

Vehicle to Everything (V2X) communications and services have sparked considerable interest as a potential component of future Intelligent Transportation Systems. V2X serves to organise communication and interaction between vehicle to vehicle (V2V), vehicle to infrastructure (V2I), vehicle to pedestrians (V2P), and vehicle to networks (V2N). However, having multiple communication channels can generate a vast amount of data for processing and distribution. In addition, V2X services may be subject to performance requirements relating to dynamic handover and low latency communication channels. Good throughput, lower delay, and reliable packet delivery are the core requirements for V2X services. Edge Computing (EC) may be a feasible option to address the challenge of dynamic handover and low latency to allow V2X information to be transmitted across vehicles. Currently, existing comparative studies do not cover the applicability of EC for V2X. This review explores EC approaches to determine the relevance for V2X communication and services. EC allows devices to carry out part or all of the data processing at the point where data is collected. The emphasis of this review is on several methods identified in the literature for implementing effective EC. We describe each method individually and compare them according to their applicability. The findings of this work indicate that most methods can simulate the EC positioning under predefined scenarios. These include the use of Mobile Edge Computing, Cloudlet, and Fog Computing. However, since most studies are carried out using simulation tools, there is a potential limitation in that crucial data in the search for EC positioning may be overlooked and ignored for bandwidth reduction. The EC approaches considered in this work are limited to the literature on the successful implementation of V2X communication and services. The outcome of this work could considerably help other researchers better characterise EC applicability for V2X communications and services.
Crystal structure of Pseudomonas aeruginosa FabB C161A, a template for structure-based design for new antibiotics
Authors: Yadrykhins'ky Vladyslav , Georgiou Charis , Brenk Ruth 
Published: November 2021

Background: FabB (3-oxoacyl-[acyl-carrier-protein] synthase 1) is part of the fatty acid synthesis II pathway found in bacteria and a potential target for antibiotics. The enzyme catalyses the Claisen condensation of malonyl-ACP (acyl carrier protein) with acyl-ACP via an acyl intermediate. Here, we report the crystal structure of the intermediate-mimicking Pseudomonas aeruginosa FabB (PaFabB) C161A variant. Methods: His-tagged PaFabB C161A was expressed in E.coli Rosetta DE3 pLysS cells, cleaved by TEV protease and purified using affinity and size exclusion chromatography. Commercial screens were used to identify suitable crystallization conditions which were subsequently improved to obtain well diffracting crystals. Results: We developed a robust and efficient system for recombinant expression of PaFabB C161A. Conditions to obtain well diffracting crystals were established. The crystal structure of PaFabB C161A was solved by molecular replacement at 1.3 Å resolution. Conclusions: The PaFabB C161A crystal structure can be used as a template to facilitate the design of FabB inhibitors.
Does Water Scarcity Affect Environmental Performance? Evidence from Manufacturing Facilities in Texas
Authors: Muthulingam Suresh , Dhanorkar Suvrat , Corbett Charles J. 
Published: November 2021

It is well known that manufacturing operations can affect the environment, but hardly any research explores whether the natural environment shapes manufacturing operations. Specifically, we investigate whether water scarcity, which results from environmental conditions, influences manufacturing firms to lower their toxic releases to the environment. We created a data set that spans 2000–2016 and includes details on the toxic emissions of 3,092 manufacturing facilities in Texas. Additionally, our data set includes measures of the water scarcity experienced by these facilities. Our econometric analysis shows that manufacturing facilities reduce their toxic releases into the environment when they have experienced drought conditions in the previous year. We examine facilities that release toxics to water as well as facilities with no toxic releases to water. We find that the reduction in total releases (to all media) is driven mainly by those facilities that release toxic chemicals to water. Further investigation at a more granular level indicates that water scarcity compels manufacturing facilities to lower their toxic releases into media other than water (i.e., land or air). The impact of water scarcity on toxic releases to water is more nuanced. A full-sample analysis fails to link water scarcity to lower toxic releases to water, but a further breakdown shows that manufacturing facilities in counties with a higher incidence of drought do lower their toxic releases to water. We also find that facilities that release toxics to water undertake more technical and input modifications to their manufacturing processes when they face water scarcity.This paper was accepted by David Simchi-Levi, operations management.
Two-photon scattering effect of a single Mie scatterer
Authors: Hong Peilong 
Published: November 2021

We perform a theoretical study on the two-photon scattering effect of a single Mie scatterer, and investigate its dependence on the rotation angle of the scatterer, and the wavelength and polarization of incident light. Different regions of the two-photon correlation map are recognized by investigating two parameters, i.e., the visibility of two-photon superposition that represents the path information of different two-photon paths, and the balance factor of the double-side single-photon scattering amplitude that indicates the possibility of the single-photon scattering process. It is shown that the two-photon scattering pattern is a result of two-photon interference from the incoherent to fully coherent regime, together with a contribution from a single-photon-like correlation. In the coherent two-photon interference region, it is found that the transition from destructive to constructive interference emerges by rotating the scatterer, as well as by changing the polarization of incident light. These results demonstrate the rich features of the two-photon scattering effect for a single Mie scatterer, and open new possibilities to explore nano scatterers for controlling two-photon interference, and their applications in communications, sensing, and imaging.
A novel high performing multiplex immunoassay Multi-HTLV for serological confirmation and typing of HTLV infections
Authors: Marqué Lola , Liehl Peter , De Boer Jasper , Pottel Hans , Murphy Edward L. , Bruhn Roberta , Stone Mars , Kaidarova Zhanna , Lee Tzong-Hae , Busch Michael , Zrein Maan 
Published: November 2021

Background
Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated 5 to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated cellular blood components. HTLV-associated syndromes are considered as neglected diseases for which there are no vaccines or therapies available, making it particularly important to ensure the best possible diagnosis to enable proper counselling of infected persons and avoid secondary transmission. Although high quality antibody screening assays are available, currently available confirmatory tests are costly and have variable performance, with high rates of indeterminate and non-typable results reported in many regions of the world. The objective of this project was to develop and validate a new high-performance multiplex immunoassay for confirmation and discrimination of HTLV-1 and HTLV-2 strains.

Methodology/Principal findings
The multiplex platform was used first as a tool to identify suitable antigens and in a second step for assay development. With data generated on over 400 HTLV-positive blood donors sourced from USA and French blood banks, we developed and validated a high-precision interpretation algorithm. The Multi-HTLV assay demonstrated very high performance for confirmation and strain discrimination with 100% sensitivity, 98.1% specificity and 100% of typing accuracy in validation samples. The assay can be interpreted either visually or automatically with a colorimetric image reader and custom algorithm, providing highly reliable results.

Conclusions/Significance
The newly developed Multi-HTLV is very competitive with currently used confirmatory assays and reduces considerably the number of indeterminate results. The multiparametric nature of the assay opens new avenues to study specific serological signatures of each patient, follow the evolution of infection, and explore utility for HTLV disease prognosis. Improving HTLV diagnostic testing will be critical to reduce transmission and to improve monitoring of seropositive patients.