bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of oncogene Activin A receptor type I by Helicobacter pylori infection promotes gastric intestinal metaplasia via regulating CDX2
Authors: Chen Hong‐Yan , Hu Yi , Xu Xin‐Bo , Zhou Yan‐An , Li Nian‐Shuang , He Cong , Xie Chuan , Lu Nong‐Hua , Zhu Yin 
Published: September 2021

AbstractBackgroundActivin A receptor type I (ACVR1) is involved in tumorigenesis. However, the underlying molecular mechanisms of ACVR1 in gastric cancer (GC) and its association with Helicobacter pylori remained unclear.Materials and methodsThe Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA) database were utilized to explore the ACVR1 expression in GC and normal control and its association with survival. The ACVR1 was knocked out using CRISPR/Cas‐9; RNA sequencing analysis was performed in AGS cells with ACVR1 knockout and normal control. Functional experiments (CCK‐8, colony‐forming, and transwell assays) were conducted to demonstrate the role of ACVR1 in cell proliferation, invasion, and metastasis. H. pylori‐infected C57/BL6 models were established. ACVR1, p‐Smad1/5, and CDX2 were detected in AGS cells cocultured with H. pylori strains. The CDX2 and key elements of BMP signaling pathway were detected in AGS cells with ACVR1 knockout and normal control. In addition, Immunohistochemistry was performed to detect the ACVR1 and CDX2 expression in gastric samples.ResultsACVR1 expression was higher in GC than normal control from TCGA, GEPIA, and samples collected from our hospital (p < 0.05). ACVR1 promoted cell proliferation, migration, and invasion in vitro. Both cagA+ and cagA− H. pylori could upregulate the expression ACVR1 (p < 0.05). Downregulation of ACVR1 inhibited the H. pylori‐induced cell proliferation, migration, and invasion (p < 0.05). H. pylori increased the expression of p‐Smad 1/5 and CDX2. The CDX2 and key elements of BMP signaling pathway were downregulated in AGS cells with ACVR1 knockout. ACVR1 and CDX2 were upregulated in the stage of intestinal metaplasia (IM). Moreover, ACVR1 and CDX2 expressions were higher in H. pylori‐positive group than H. pylori‐negative group (p < 0.05).ConclusionOur data indicate that H. pylori infection increases ACVR1 expression, promoting gastric IM via regulating CDX2, which is an essential step in H. pylori carcinogenesis.
In vitro caecum fermentation and in vivo (Gallus gallus) of calcium delivery systems fabricated by desalted duck egg white peptides and chitosan oligosaccharide on gut health
Authors: Zhao Mengge , Ma Aimin , He Hui , Zhang Xing , Jia Lei , Hou Tao 
Published: September 2021

AbstractAs calcium supplements, two novel calcium delivery systems were prepared with desalted duck egg white peptides (DPs) and chitosan oligosaccharide (COS) by Amadori‐linkage and transglutaminase (TGase)‐induced reaction, with high calcium‐binding ability of promoting calcium absorption in vivo and in vitro. However, there are relatively few studies on the gut health of calcium delivery systems. In this experiment, in vitro caecum fermentation experiment and Gallus gallus intra amniotic feeding model were used to assess the gut health of calcium delivery systems and phytic acid. Results showed that two calcium delivery systems improved the microbial community structure by inhibiting the proliferation of Escherichia coli, and promoting the proliferation of Bifidobacterium and Lactobacillus. The addition of calcium delivery systems significantly increased short chain fatty acids (SCFAs) and improved the boundary membrane function of brush nutrients by significantly increasing villi structure, surface area, cup cell expansion and production of associated genes (P < 0.05). Moreover, calcium delivery systems present significant reverse of the inhibitory effect of phytic acid on gut health through in vitro and in vivo experiments. This study suggested that DPs‐COS‐Ca systems were superior to the commercially available calcium supplement CaCO3 and had excellent calcium absorption and gut health regulating effects.
Recognition of Philadelphia chromosome‐like acute lymphoblastic leukemia as part of routine diagnostic work‐up
Authors: Podgornik Helena , Doplihar Kebe Ana , Klun Jurka , Reberšek Katarina , Šućurović Sandra , Škerget Matevž , Zver Samo 
Published: September 2021

AbstractIntroductionPhiladelphia chromosome (Ph)‐like acute lymphoblastic leukemia (ALL) is a biologically and clinically challenging subtype of B‐cell ALL which has been incorporated into the 2016 revision of the World Health classification of acute leukemia. It is independently associated with poor outcome. As it can only be reliably detected by expression profiling, it is difficult to diagnose with routine methods. Its recognition has become of greater importance due to prognostication and even more due to the new diagnostic options given by targeted therapies. There is still no standardized diagnostic test enabling its prompt recognition. Here, we introduce our approach how to detect it by combination of widely available techniques.Methods179 ALL patients diagnosed in our center during the last 8 years were included. Data on immunophenotype and cytogenetics were used to select patients with potentially Ph‐like ALL (65/179). CRLF2 gene rearrangement (CRLF2‐r) was tested by FISH in 59/65 patients, and next‐generation sequencing was done by Archer FusionPlex ALL kit in 34 patients. TSLPR expression was determined in 20 patients.ResultsPhiladelphia chromosome‐like aberrations were confirmed in 9 patients. In 10% of tested samples, CRLF2‐r was confirmed. Due to a lack of material, NGS was done only in a half of potentially Ph‐like cases. In 10%, other Ph‐like fusions were found by NGS.ConclusionsThe obtained frequencies, and genetic and patients' characteristics are in concordance with the literature data, ensuring a reliable detection of this challenging ALL subtype. The proposed algorithm allows detection of Ph‐like ALL at reasonable cost and acceptable workload.
Competition, stress and benefits: Trees and crops in the transition zone of a temperate short rotation alley cropping agroforestry system
Authors: Swieter Anita , Langhof Maren , Lamerre Justine 
Published: September 2021

AbstractTree strips on agricultural production sites offer many economic, ecological and social advantages. However, the introduction of trees creates a transition zone between tree strips and crop land. Here, trees and crop plants compete for resources such as space, nutrients, water and light, which causes stress in the low‐competitive system. On the other hand, facilitation such as additional nutrient input through tree leaf litter and fine roots are possible. This study aims to provide indications for competition and benefits that can arise for plants growing in the transition zone of a temperate short rotation alley cropping agroforestry system (SRACS). Various climatic and plant‐growth parameters were investigated between 2013 and 2019 at different positions of an SRACS with fast‐growing poplars in northern Germany. Reduced yield of wheat, oilseed rape and silage maize close to the tree strip was associated with greater soil water tension in 30 and 60 cm soil depth due to the presence of poplar roots, reduced solar radiation due to tree shading and leaf litter coverage. In contrast, poplars growing in the outer rows produced more biomass than those in the inner rows due to the additional availability of space, light and nutrients taken from the crop field. Trees in the transition zone seem to be competitive with arable crops, but without effect on the average long‐term yield of arable crops.
The impact of age and 24‐h blood pressure on arterial health in acute ischemic stroke patients: The Norwegian stroke in the young study
Authors: Saeed Sahrai , Waje‐Andreassen Ulrike , Naess Halvor , Fromm Annette , Nilsson Peter M. 
Published: September 2021

AbstractThe impact of age and 24‐h ambulatory blood pressure (ABPM) on arterial stiffness and carotid intima‐media thickness (cIMT) in ischemic stroke patients younger than 60 years of age is poorly explored. A total of 385 acute ischemic stroke patients (aged 49.6±9.7 years, 68% men) were prospectively included and grouped in younger (15–44 years, n = 93) and middle‐aged (45–60 years, n = 292). Arterial stiffness was measured by carotid‐femoral pulse wave velocity (PWV), and cIMT by carotid ultrasound. 24‐h ABPM was recorded. The middle‐aged stroke patients had higher prevalence of smoking, hypertension, diabetes mellitus, metabolic syndrome and hypercholesterolemia, and had higher PWV and cIMT (all p < .05). In multivariable linear regression analyses adjusted for sex, BMI, smoking, diabetes mellitus, total cholesterol, high‐density lipoprotein cholesterol, triglycerides, eGFR, systolic BP and concomitant antihypertensive treatment, 1SD (4.4 years) higher age was associated with higher PWV (β = 0.44,R2 = 0.46, p < .001) in the younger group, and with higher mean cIMT (β = 0.16, R2 = 0.21, p = .01) in the middle‐aged group. In the middle‐aged group, 24‐h pulse pressure had a significant association with PWV (β = 0.18, R2 = 0.19, p = .009), while the association with cIMT was attenuated (β = 0.13, R2 = 0.16, p = .065). 24‐h diastolic BP was associated with higher cIMT in the middle‐aged group (β = 0.24, p < .001, R2 = 0.23), but not with PWV in either age groups. Among ischemic stroke patients < 60 years, higher age was associated with increased arterial stiffness for patients up to age 44 years, and with cIMT in middle‐aged patients. 24‐h pulse pressure was associated with arterial stiffness, and 24‐h diastolic BP was associated with cIMT only in middle‐aged patients.
Myeloid malignancies with translocation t(4;12)(q11‐13;p13): molecular landscape, clonal hierarchy and clinical outcomes
Authors: Parinet Vincent , Chapiro Elise , Bidet Audrey , Gaillard Baptiste , Maarek Odile , Simon Laurence , Lefebvre Christine , Defasque Sabine , Mozziconacci Marie‐Joelle , Quinquenel Anne , Decamp Matthieu , Lifermann François , Ali‐Ammar Nadia , Maillon Agathe , Baron Marine , Martin Mélanie , Struski Stéphanie , Penther Dominique , Micol Jean‐Baptiste , Auger Nathalie , Bilhou‐Nabera Chrystèle , Martignoles Jean‐Alain , Tondeur Sylvie , Nguyen‐Khac Florence , Hirsch Pierre , Roos‐Weil Damien ,   on behalf the FILO (French Innovative Leukemia Organization), GFCH (Groupe Francophone de Cytogénétique Hématologique) groups
Published: September 2021

AbstractTranslocation t(4;12)(q11‐13;p13) is a recurrent but very rare chromosomal aberration in acute myeloid leukaemia (AML) resulting in the non‐constant expression of a CHIC2/ETV6 fusion transcript. We report clinico‐biological features, molecular characteristics and outcomes of 21 cases of t(4;12) including 19 AML and two myelodysplastic syndromes (MDS). Median age at the time of t(4;12) was 78 years (range, 56–88). Multilineage dysplasia was described in 10 of 19 (53%) AML cases and CD7 and/or CD56 expression in 90%. FISH analyses identified ETV6 and CHIC2 region rearrangements in respectively 18 of 18 and 15 of 17 studied cases. The t(4;12) was the sole cytogenetic abnormality in 48% of cases. The most frequent associated mutated genes were ASXL1 (n = 8/16, 50%), IDH1/2 (n = 7/16, 44%), SRSF2 (n = 5/16, 31%) and RUNX1 (n = 4/16, 25%). Interestingly, concurrent FISH and molecular analyses showed that t(4;12) can be, but not always, a founding oncogenic event. Median OS was 7.8 months for the entire cohort. In the 16 of 21 patients (76%) who received antitumoral treatment, overall response and first complete remission rates were 37% and 31%, respectively. Median progression‐free survival in responders was 13.7 months. Finally, t(4;12) cases harboured many characteristics of AML with myelodysplasia‐related changes (multilineage dysplasia, MDS‐related cytogenetic abnormalities, frequent ASXL1 mutations) and a poor prognosis.
Emerging roles of circular RNAs in osteoporosis
Authors: Chen Weichun , Zhang Baozhong , Chang Xiao 
Published: September 2021

AbstractOsteoporosis is one bone disease characterized with skeletal impairment, bone strength reduced and fracture risk enhanced. The regulation processes of bone metabolism are associated with several factors such as mechanical stimulation, epigenetic regulation and hormones. However, the mechanism of osteoporosis remains unsatisfactory. Increasing high‐throughput RNA sequencing and circular RNAs (circRNAs) microarray studies indicated that circRNAs are differentially expressed in osteoporosis. Growing functional studies further pinpointed specific deregulated expressed circRNAs (e.g., circ_28313, circ_0016624, circ_0006393, circ_0076906 and circ_0048211) for their functions involved in bone metabolism, including bone marrow stromal cells (BMSCs) differentiation, proliferation and apoptosis. Moreover, CircRNAs (circ_0002060, Circ_0001275 and Circ_0001445) may be acted as diagnostic biomarkers for osteoporosis. This review discussed recent progresses in the circRNAs expression profiling analyses and their potential functions in regulating BMSCs differentiation, proliferation and apoptosis.
Developmental trajectories of empathic concern in infancy and their links to social competence in early childhood
Authors: Paz Yael , Davidov Maayan , Orlitsky Tal , Roth‐Hanania Ronit , Zahn‐Waxler Carolyn 
Published: September 2021

BackgroundEmpathic concern is an important component of children’s social competence. Yet, little is known about the role of the development of concern for others during infancy as a predictor of social competence in early childhood.MethodsIsraeli infants (N = 165, 50% girls) were observed five times, from 3 to 36 months. Empathic concern was assessed at ages 3–18 months using observations, and four components of social competence were assessed at 36 months using observations and teacher reports.ResultsFour groups with distinct developmental trajectories of empathic concern from 3 to 18 months were identified: early‐onset (starting high and increasing), low‐empathy (starting low with minimal increase), rising (starting low and increasing considerably), and a very small group with a negative slope (decreasing). The first three trajectories differed on aspects of social competence at 36 months. Early‐onset children continued to exhibit the highest empathic concern. Both the early‐onset and rising groups had greater affective knowledge than the low‐empathy group. Moreover, the rising group had better peer relations compared with low‐empathy trajectory children.ConclusionsChildren who exhibit high levels of empathy early in infancy are likely to show high social competence later on. However, even when initial empathy levels are low, subsequent growth in empathy from 3 to 18 months can occur, with positive consequences for children’s social competence at 36 months. Only children with low initial empathic concern and minimal growth across infancy are at increased risk of having poorer socioemotional capabilities in early childhood.
Classification of bananas during ripening using peel roughness analysis—An application of atomic force microscopy to food process
Authors: Khodabakhshian Rasool , Baghbani Reza 
Published: September 2021

AbstractTo monitor banana surface (peel) roughness changes during ripening treatment, atomic force microscopy (AFM) as a novel and emerging technique was used in this study. The roughness of banana peel was studied using the arithmetic mean between peaks and troughs (Ra) and the root‐mean‐square roughness (Rq). It was concluded that with changing the ripening stages, the behavior of the roughness changes significantly. With advancing fruit ripening, the extending of the epicarp and the decrease of the surface (peel) roughness were found due to enlarging of fruit volume. The highest mean roughness was found to be at stage 1, Ra = 8.25 and Rq = 9.65 nm. Based on two‐dimensional profile results, the surface (peel) roughness was affected strongly by studied different ripening stages. It was concluded that the peak values in the ripple profiles become smaller with advancing fruit ripening. However, the effects of noise in the profiles appeared to increase. So, in all the initial stages of banana fruit ripening, the noise was found to be minimal. Consequently, the AFM technique was found to be a promising tool for quantification of the peel roughness or glossiness and also could help in the quality control of banana fruit on the nanoscale.
Assessment of physical and aerodynamic properties of corn kernel (KSC 704)
Authors: Ahmadi Chenarbon Hossein , Movahhed Sara 
Published: September 2021

AbstractPhysical and aerodynamic properties of agricultural products have been used to precision design of the machinery and different postharvest operations. In this study, some physical and aerodynamic properties of corn kernels were investigated as a function of size at three levels of small, medium, and large (8.41 mm, 9.60 mm, and 11.62 mm, respectively) and moisture content in the range of 10 to 25% (wet basis). According to results, with increasing the moisture content from 10 to 25%, the length; width; thickness; geometric mean diameter; mass; and volume of the small, medium, and large kernels linearly increased by 2.22%, 2.40%, and 1.68%; 3.76%, 1.82%, and 1.55%; 3.98%, 2.49%, and 2.67%; 3.29%, 2.23%, and 1.93%; 8.44%, 7.38%, and 8.12%; and 11.32%, 10.71%, and 10.21%, respectively. In this regard, the projection area of the specimens increased nonlinearly by 6.06%, 4.26%, and 3.26%, respectively; whereas, true density decreased nonlinearly by 2.65%, 2.91%, and 1.90%, respectively. The maximum terminal velocity (12.41 m/s) and Reynolds number (6,037.44) were observed in large kernels and the moisture content of 25%; whereas, the minimum terminal velocity (8.32 m/s) and Reynolds number (3,016.55) were observed in small kernels and the moisture content of 10%. Maximum and minimum drag coefficients (1.014 vs. 0.302) were observed in small kernels at the moisture of 10% and large kernels at the moisture of 25%, respectively. The polynomial model was chosen as a suitable model in order to foretell the terminal velocity, Reynolds number, and drag coefficient of kernels as a function of moisture content and size.Practical ApplicationsMechanical damages to seeds at harvesting, transporting, and threshing are major concerns for farmers and farming experts. All the processes of wind transfer, flotation, and seed removal from seed mixture and the stalks and husks depend on the behavior of the seeds in the wind flow. Therefore, it is crucial to determine the physical and aerodynamic properties of various crops to analyzing their behavior during transport, processing, and precise design of farm equipment and machines to minimize wastes.
Families' experiences of grief and bereavement in the emergency department: A scoping review
Authors: Ito Yoshiyasu , Tsubaki Michihiro , Kobayashi Masahiro 
Published: September 2021

AbstractAimDeath is a frequent event in the emergency department, and many family members experience grief and bereavement. However, little is known about what families experience. The aim of this study was to explore previous findings about families’ experiences of grief and bereavement in the emergency department to clarify and understand their experience.MethodsA scoping review was chosen as the design for this research. Studies were searched from four electronic databases. The obtained studies were screened independently by two reviewers and selected by mutual agreement of the entire team based upon the eligibility criteria. All relevant data were extracted, and thematic analysis was conducted to assess families' grief and bereavement experiences in the emergency department.ResultsThe database searches initially resulted in 982 studies; these were finally narrowed to 20 studies for data extraction. Publication years ranged from 1987 to 2020. The studies were conducted across nine countries; most were from the United States and had a qualitative research design. Analysis elicited the following themes: “lack of information,” “breaking bad news,” “being present during resuscitation,” “chaotic environment,” “psychosocial reaction in bereavement,” and “support and care needs from healthcare professionals.”ConclusionThese themes reveal challenges in providing support and care from emergency nurses to bereaved families, including inadequate provision of information, poor design of the emergency department due to the lack of privacy, and families’ unmet support and care needs. Emergency nurses need to better understand families’ experience of grief and bereavement.
Factors affecting the quality of dementia care at acute care hospitals: A cross‐sectional study
Authors: Urashima Shoko , Greiner Chieko , Ryuno Hirochika , Yamaguchi Yuko 
Published: September 2021

AbstractAim and ObjectivesThe purpose of this study was to investigate which factors are associated with the quality of dementia care in acute care hospitals.BackgroundThe number of people with dementia who are admitted to acute care hospitals is increasing. Improving the quality of dementia care in acute care hospitals is an important issue. Prior studies have demonstrated that not only knowledge and nursing experience, but also psychological factors and the nursing practice environment are related to high‐quality care on general wards.DesignCross‐sectional hypothesis‐testing design.MethodsParticipants were nurses providing care to people with dementia at acute care hospitals. Questionnaires were distributed to 300 acute care hospitals in Japan, 10 copies each and 773 valid responses were received. Based on the hypothesis model, variables were tested using multiple regression analysis. The model described the relationship between quality of care, personal attributes and the nursing practice environment. The Strengthening the Reporting of Observational Studies in Epidemiology checklist was used.ResultsAlmost 90% of the study sample was female, the mean age was 37.4 ± 9.3 years, and the mean nursing experience was 14.0 ± 8.7 years. The results showed that nursing foundations for quality of care, staffing and resource adequacy, specialist consultation, promoting systematic recreation and exchange, knowledge, and feelings towards people with dementia were associated with the quality of dementia care in acute care hospitals. The adjusted coefficient of determination was 0.367.ConclusionThis study identified factors associated with the quality of dementia care in acute care hospitals. Knowledge and feelings towards people with dementia are important, and the nursing practice environment plays an important role in improving the quality of dementia care.Relevance to Clinical PracticeNot only improving nurse's practical ability but also a supportive nursing practice environment enhances the quality of dementia care in acute care hospitals.