bluuebunny/crossref_metadata_2025_split huggingface dataset:
HDAC Inhibition Reverses Preexisting Diastolic Dysfunction and Blocks Covert Extracellular Matrix Remodeling
Authors: Travers Joshua G. , Wennersten Sara A. , Peña Brisa , Bagchi Rushita A. , Smith Harrison E. , Hirsch Rachel A. , Vanderlinden Lauren A. , Lin Ying-Hsi , Dobrinskikh Evgenia , Demos-Davies Kimberly M. , Cavasin Maria A. , Mestroni Luisa , Steinkühler Christian , Lin Charles Y. , Houser Steven R. , Woulfe Kathleen C. , Lam Maggie P.Y. , McKinsey Timothy A. 
Published: March 2021

Background:
Diastolic dysfunction (DD) is associated with the development of heart failure and contributes to the pathogenesis of other cardiac maladies, including atrial fibrillation. Inhibition of histone deacetylases (HDACs) has been shown to prevent DD by enhancing myofibril relaxation. We addressed the therapeutic potential of HDAC inhibition in a model of established DD with preserved ejection fraction.

Methods:
Four weeks after uninephrectomy and implantation with deoxycorticosterone acetate pellets, when DD was clearly evident, 1 cohort of mice was administered the clinical-stage HDAC inhibitor ITF2357/Givinostat. Echocardiography, blood pressure measurements, and end point invasive hemodynamic analyses were performed. Myofibril mechanics and intact cardiomyocyte relaxation were assessed ex vivo. Cardiac fibrosis was evaluated by picrosirius red staining and second harmonic generation microscopy of left ventricle (LV) sections, RNA sequencing of LV mRNA, mass spectrometry–based evaluation of decellularized LV biopsies, and atomic force microscopy determination of LV stiffness. Mechanistic studies were performed with primary rat and human cardiac fibroblasts.

Results:
HDAC inhibition normalized DD without lowering blood pressure in this model of systemic hypertension. In contrast to previous models, myofibril relaxation was unimpaired in uninephrectomy/deoxycorticosterone acetate mice. Furthermore, cardiac fibrosis was not evident in any mouse cohort on the basis of picrosirius red staining or second harmonic generation microscopy. However, mass spectrometry revealed induction in the expression of >100 extracellular matrix proteins in LVs of uninephrectomy/deoxycorticosterone acetate mice, which correlated with profound tissue stiffening based on atomic force microscopy. ITF2357/Givinostat treatment blocked extracellular matrix expansion and LV stiffening. The HDAC inhibitor was subsequently shown to suppress cardiac fibroblast activation, at least in part, by blunting recruitment of the profibrotic chromatin reader protein BRD4 (bromodomain-containing protein 4) to key gene regulatory elements.

Conclusions:
These findings demonstrate the potential of HDAC inhibition as a therapeutic intervention to reverse existing DD and establish blockade of extracellular matrix remodeling as a second mechanism by which HDAC inhibitors improve ventricular filling. Our data reveal the existence of pathophysiologically relevant covert or hidden cardiac fibrosis that is below the limit of detection of histochemical stains such as picrosirius red, highlighting the need to evaluate fibrosis of the heart using diverse methodologies.
Ischemic Core Overestimation on Computed Tomography Perfusion
Authors: García-Tornel Álvaro , Campos Daniel , Rubiera Marta , Boned Sandra , Olivé-Gadea Marta , Requena Manuel , Ciolli Ludovico , Muchada Marian , Pagola Jorge , Rodriguez-Luna David , Deck Matias , Juega Jesus , Rodríguez-Villatoro Noelia , Sanjuan Estela , Tomasello Alejandro , Piñana Carlos , Hernández David , Álvarez-Sabin José , Molina Carlos A. , Ribó Marc 
Published: March 2021

Background and Purpose:
Different studies have pointed that CT perfusion (CTP) could overestimate ischemic core in early time window. We aim to evaluate the influence of time and collateral status on ischemic core overestimation.

Methods:
Retrospective single-center study including patients with anterior circulation large-vessel stroke that achieved reperfusion after endovascular treatment. Ischemic core and collateral status were automatically estimated on baseline CTP using commercially available software. CTP-derived core was considered as tissue with a relative reduction of cerebral blood flow <30%, as compared with contralateral hemisphere. Collateral status was assessed using the hypoperfusion intensity ratio (defined by the proportion of the time to maximum of tissue residue function >6 seconds with time to maximum of tissue residue function >10 seconds). Final infarct volume was measured on 24 to 48 hours noncontrast CT. Ischemic core overestimation was considered when CTP-derived core was larger than final infarct.

Results:

Four hundred and seven patients were included in the analysis. Median CTP-derived core and final infarct volume were 7 mL (interquartile range, 0–27) and 20 mL (interquartile range, 5–55), respectively. Median hypoperfusion intensity ratio was 0.46 (interquartile range, 0.23–0.59). Eighty-three patients (20%) presented ischemic core overestimation (median overestimation, 12 mL [interquartile range, 41–5]). Multivariable logistic regression analysis adjusted by CTP-derived core and confounding variables showed that poor collateral status (per 0.1 hypoperfusion intensity ratio increase; adjusted odds ratio, 1.41 [95% CI, 1.20–1.65]) and earlier onset to imaging time (per 60 minutes earlier; adjusted odds ratio, 1.14 [CI, 1.04–1.25]) were independently associated with core overestimation. No significant association was found with imaging to reperfusion time (per 30 minutes earlier; adjusted odds ratio, 1.17 [CI, 0.96–1.44]). Poor collateral status influence on core overestimation differed according to onset to imaging time, with a stronger size of effect on early imaging patients(
P
interaction
<0.01).

Conclusions:
In patients with large-vessel stroke that achieve reperfusion after endovascular therapy, poor collateral status might induce higher rates of ischemic core overestimation on CTP, especially in patients in earlier window time. CTP reflects a hemodynamic state rather than tissue fate; collateral status and onset to imaging time are important factors to consider when estimating core on CTP.
A new species, Sursamucro rostratus sp. nov. (Copepoda, Calanoida) from the abyss of the northern Pacific and South Atlantic
Authors: Markhaseva Elena L. , Bradford-Grieve Janet M. , Renz Jasmin 
Published: March 2021

Abstract
A new calanoid copepod, Sursamucro rostratus sp. nov., belonging to the family Aetideidae, is described from female specimens collected in the northern Pacific and South Atlantic from abyssal waters close to the sea bed. The new species is the second of a rare deep-water genus, Sursamucro; it differs from its congener in size, configuration of the rostrum, the shape of the posterior corners of the prosome and details of antenna, mandible, maxillule and P1 setation. The genus Sursamucro is recorded for the first time from the northern Pacific and the Atlantic Ocean.
Role of circulating tumor cells in diagnosis of lung cancer: a systematic review and meta-analysis
Authors: Zhao Qingtao , Yuan Zheng , Wang Huien , Zhang Hua , Duan Guochen , Zhang Xiaopeng 
Published: March 2021

Objective We systematically reviewed the literature relating to the diagnostic accuracy of circulating tumor cells (CTCs) for the clinical determination of lung cancer. Methods This meta-analysis aimed to evaluate the diagnostic accuracy of CTCs for the clinical determination of lung cancer. The PubMed, Embase, Cochrane Library, and Web of Science databases were searched for relevant studies up to 31 May 2020. The numbers of patients with true positive, false positive, false negative, and true negative results were extracted from each individual study. Pooled sensitivity, specificity, and area under the curve values were calculated with 95% confidence intervals (CI). Results Twenty-one studies with 3997 subjects met the inclusion criteria. The overall diagnostic accuracy was assessed. The pooled sensitivity and specificity were 0.72 (95%CI: 0.65–0.79) and 0.96 (95%CI: 0.91–0.98), respectively, and the pooled positive and negative likelihood ratios were 16.86 (95%CI: 7.65–37.12) and 0.29 (95%CI: 0.23–0.37), respectively. The combined diagnostic odds ratio was 58.12 (95%CI: 24.82–136.09). Conclusion This meta-analysis indicated that CTCs had good diagnostic value for detecting lung cancer.
Prognostic value of the albumin–globulin ratio and albumin–globulin score in patients with multiple myeloma
Authors: Cai Ying , Zhao Yu , Dai Qiuxin , Xu Maozhong , Xu Xin , Xia Wenkai 
Published: March 2021

Objective The albumin–globulin ratio (AGR) has been identified as a promising prognostic predictor of mortality in patients with hematological malignancies. This study investigated the prognostic significance of AGR in patients with multiple myeloma. Methods Two hundred patients diagnosed with multiple myeloma from January 2010 to October 2018 were retrospectively analyzed and followed up until December 2019. Kaplan–Meier curves and multivariate Cox regression analysis were applied to detect the prognostic value of AGR. Results The median follow-up period was 36 months. The optimal cutoff of AGR was 1.16 according to receiver operating characteristic curve analysis. High AGR was significantly correlated with better overall survival (OS) and progression-free survival (PFS). Multivariate analysis revealed that low AGR was an independent prognostic factor for worse OS (hazard ratio [HR] = 1.82, 95% confidence interval [CI] = 1.15–2.94) and PFS (HR = 1.53, 95% CI = 1.09–2.17). Conclusions AGR may represent a potential prognostic biomarker in patients with multiple myeloma. Mini Abstract: We demonstrated that high AGR was associated with a favorable overall survival and progression-free survival in patients with multiple myeloma.
Evaluation of static and dynamic Pupillometry changes in men using Silodosin for benign prostatic hypertrophy
Authors: Karaca Umut , Kaya Engin , Ayyildiz Onder , Ozge Gokhan , Kucukevcilioglu Murat , Usta Gulsah , Mutlu Fatih Mehmet 
Published: March 2021

Abstract
Background
Intraoperative floppy iris syndrome is a variant of the small pupil syndrome that has been observed during cataract surgery in some patients currently or previously treated with α1 adrenergic blockers. It is important for cataract surgeons to predict the probable complications preoperatively. Our study aims to evaluate the static and dynamic pupil characteristics of patients treated with silodosin—a selective α1 adrenergic blocker—for benign prostate hypertrophy (BPH) and to compare these values with healthy subjects using an automatic quantitative pupillometry system.

Methods
A total of 74 BPH patients treated with silodosin for six months (group 1) and 30 healthy subjects (group 2) were enrolled in this prospective multidisciplinary cross-sectional study. Static and dynamic pupillometric measurements were obtained under optimized conditions, and the results were compared between the two groups.

Results
Seventy-four male patients with a mean age of 63,35 ± 7,21 (46–77) years with BPH treated with silodosin and 30 normal male subjects with a mean age of 63,07 ± 4,73 (52–71) years were analyzed. There were statistically significant differences between the groups with regard to scotopic pupil diameter (PD), high photopic PD, and low photopic PD (p < 0.001, for each one). The patient group had statistically significant higher values of amplitude and velocity of pupil contraction and lower values of duration of pupil contraction and latency as well as duration and velocity of pupil dilation.

Conclusion
The static and dynamic pupil characteristics of subjects treated with silodosin for BPH are different from those of healthy eyes. In addition, our results may have shed light on the risk for intraoperative floppy iris syndrome (IFIS) before cataract surgery; thus, surgeons can be alert and take precautions.
The volume of general surgery emergency cases in a government hospital during the COVID-19 pandemic and two other periods: a comparative, retrospective study
Authors: Abu Shakra Ibrahim , Bez Maxim , Ganam Samer , Francis Rola , Muati Amir , Bickel Amitai , Merei Fahed , Talmi Ziv , Kamal Khatib , Kakiashvili Eli 
Published: March 2021

Abstract
Background
During March and April 2020, reductions in non-COVID-19 hospital admissions were observed around the world. Elective surgeries, visits with general practitioners, and diagnoses of medical emergencies were consequently delayed.

Objective

To compare the characteristics of patients admitted to a northern Israeli hospital with common surgical complaints during three periods: the lockdown due to the COVID-19 outbreak, the Second Lebanon War in 2006, and a regular period.

Methods
Demographic, medical, laboratory, imaging, intraoperative, and pathological data were collected from electronic medical files of patients who received emergency treatment at the surgery department of a single hospital in northern Israel. We retrospectively compared the characteristics of patients who were admitted with various conditions during three periods.

Results

Patients’ mean age and most of the clinical parameters assessed were similar between the periods. However, pain was reportedly higher during the COVID-19 than the control period (8.7 vs. 6.4 on a 10-point visual analog scale, P < 0.0001). During the COVID-19 outbreak, the Second Lebanon War, and the regular period, the mean numbers of patients admitted daily were 1.4, 4.4, and 3.0, respectively. The respective mean times from the onset of symptoms until admission were 3, 1, and 1.5 days, P < 0.001. The respective proportions of surgical interventions for appendiceal disease were 95%, 96%, and 69%; P = 0.03.

Conclusions
Compared to a routine period, patients during the COVID-19 outbreak waited longer before turning to hospitalization, and reported more pain at arrival. Patients during both emergency periods were more often treated surgically than non-operatively.
Long-term outcome of a patient with Transcobalamin deficiency caused by the homozygous c.1115_1116delCA mutation in TCN2 gene: a case report
Authors: Martino Francesco , Magenta Alessandra , Troccoli Maria Letizia , Martino Eliana , Torromeo Concetta , Putotto Carolina , Barillà Francesco 
Published: March 2021

Abstract
Background
Transcobalamin deficiency is a rare autosomal recessive inborn error of cobalamin transport (prevalence: < 1/1000000) which clinically manifests in early infancy.

Case presentation
We describe the case of a 31 years old woman who at the age of 30 days presented with the classical clinical and laboratory signs of an inborn error of vitamin B12 metabolism. Family history revealed a sister who died at the age of 3 months with a similar clinical syndrome and with pancytopenia. She was started on empirical intramuscular (IM) cobalamin supplements (injections of hydroxocobalamin 1 mg/day for 1 week and then 1 mg twice a week) and several transfusions of washed and concentrated red blood cells. With these treatments a clear improvement in symptoms was observed, with the disappearance of vomiting, diarrhea and normalization of the full blood count. At 8 years of age injections were stopped for about two and a half months causing the appearance of pancytopenia. IM hydroxocobalamin was then restarted sine die. The definitive diagnosis could only be established at 29 years of age when a genetic evaluation revealed the homozygous c.1115_1116delCA mutation of TCN2 gene (p.Q373GfsX38).
Currently she is healthy and she is taking 1 mg of IM hydroxocobalamin once a week.

Conclusions
Our case report highlights that early detection of TC deficiency and early initiation of aggressive IM treatment is likely associated with disease control and an overall favorable outcome.
Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts
Authors: Ali Sameh Samir , Al-Tohamy Rania , Koutra Eleni , Kornaros Michael , Khalil Maha , Elsamahy Tamer , El-Shetehy Mohamed , Sun Jianzhong 
Published: March 2021

Abstract
Background
Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. However, an effective and environmentally friendly treatment method has not yet been implemented, while concurrently, the increasing demand of modern societies for adequate and sustainable energy supply still remains a global challenge. Under this scope, the purpose of the present study was to isolate promising species of yeasts inhabiting wood-feeding termite guts, for combined azo dyes and textile wastewater bioremediation, along with biodiesel production.

Results
Thirty-eight yeast strains were isolated, molecularly identified and subsequently tested for desired enzymatic activity, lipid accumulation, and tolerance to lignin-derived metabolites. The most promising species were then used for construction of a novel yeast consortium, which was further evaluated for azo dyes degradation, under various culture conditions, dye levels, as well as upon the addition of heavy metals, different carbon and nitrogen sources, and lastly agro-waste as an inexpensive and environmentally friendly substrate alternative. The novel yeast consortium, NYC-1, which was constructed included the manganese-dependent peroxidase producing oleaginous strains Meyerozyma caribbica, Meyerozyma guilliermondii, Debaryomyces hansenii, and Vanrija humicola, and showed efficient azo dyes decolorization, which was further enhanced depending on the incubation conditions. Furthermore, enzymatic activity, fatty acid profile and biodiesel properties were thoroughly investigated. Lastly, a dye degradation pathway coupled to biodiesel production was proposed, including the formation of phenol-based products, instead of toxic aromatic amines.

Conclusion
In total, this study might be the first to explore the application of MnP and lipid-accumulating yeasts for coupling dye degradation and biodiesel production.
Do No Harm in refugee humanitarian aid: the case of the Rohingya humanitarian response
Authors: Khaled Abu Faisal Md. 
Published: March 2021

AbstractThe article broadly examines how humanitarian aid for Rohingya refugees inadvertently harmed poorer hosts and adversely affected local capacities for peace. The article also discusses possible ways of easing tension and improving social cohesion in the refugee-hosting areas, while also highlighting how policy- and mandate-related constraints hinder a humanitarian response anchored in the "Do No Harm" principle. Finally, the article concludes with the argument that the humanitarian agencies should not just limit themselves to identifying the unintended consequences and lapses in the intervention. Instead, the Do No Harm principle should lead humanitarian aid agencies to make an active effort to accept responsibility for the harm while taking all necessary steps to mitigate or avoid harming in future interventions.
Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia
Authors: Assadi Besma Hamrouni , Chouikhi Sabrine , Ettaib Refki , M’hamdi Naima Boughalleb , Belkadhi Mohamed Sadok 
Published: March 2021

Abstract
Background
The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia.

Results
In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6).

Conclusions
It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.
A critical review of intrusion detection systems in the internet of things: techniques, deployment strategy, validation strategy, attacks, public datasets and challenges
Authors: Khraisat Ansam , Alazab Ammar 
Published: March 2021

AbstractThe Internet of Things (IoT) has been rapidly evolving towards making a greater impact on everyday life to large industrial systems. Unfortunately, this has attracted the attention of cybercriminals who made IoT a target of malicious activities, opening the door to a possible attack on the end nodes. To this end, Numerous IoT intrusion detection Systems (IDS) have been proposed in the literature to tackle attacks on the IoT ecosystem, which can be broadly classified based on detection technique, validation strategy, and deployment strategy. This survey paper presents a comprehensive review of contemporary IoT IDS and an overview of techniques, deployment Strategy, validation strategy and datasets that are commonly applied for building IDS. We also review how existing IoT IDS detect intrusive attacks and secure communications on the IoT. It also presents the classification of IoT attacks and discusses future research challenges to counter such IoT attacks to make IoT more secure. These purposes help IoT security researchers by uniting, contrasting, and compiling scattered research efforts. Consequently, we provide a unique IoT IDS taxonomy, which sheds light on IoT IDS techniques, their advantages and disadvantages, IoT attacks that exploit IoT communication systems, corresponding advanced IDS and detection capabilities to detect IoT attacks.
Review of porous asphalt pavements in cold regions: the state of practice and case study repository in design, construction, and maintenance
Authors: Zhang Kun , Kevern John 
Published: March 2021

AbstractPorous asphalt pavement is a sustainable infrastructure tool used to benefit urban resilience. This paper summarizes the design, construction, and maintenance practices of porous asphalt pavements (PAPs) specific to cold regions. It includes discussions on the structural design considering frost depth and frost heave of subgrade soils, material selection and design for adequate freeze-thaw durability, construction of PAPs in cold weather, winter maintenance of PAPs for snow and ice control, and performance deterioration caused by other winter activities such as studded tires. Distinguished from other review works on this topic, the major contributions of this review paper employ case studies of PAPs to address design, construction, and maintenance concerns of PAPs in cold regions. These projects have demonstrated the success of using PAPs in cold regions and design practitioners can refer to these case studies for the new design and installation of PAPs in cold regions.