bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA Member TaMIR5062 in T. Aestivum Involves Plant Drought and Salt Acclimation via Regulating Physiological Processes Associated with Water Retention and ROS Homeostasis
Authors: Qiao Yuanjinzi , Wang Ling , Yu Zidi , Ni Chenyang , Li Tianjiao , Guo Chengjin , Xiao Kai 
Published: December 2021

Abstract
microRNA members negatively regulate target genes via posttranscriptional cleavage or translation repression mechanisms, impacting on plant growth, development, and stress response. In this study, we characterized TaMIR5062, a miRNA member in T. aestivum, in mediating drought and salt responses. TaMIR5062 interacts with six target genes, including two encoding calmodulins, three coding for 4-oxalocrotonate tautomerases, and one for pumilio-family RNA binding domain protein. The TaMIR5062 transcripts were gradually upregulated in plants upon 27-h drought and salt treatments, whose induced expression under stress treatments was restored following the normal recovery condition. Tobacco (N. tabacum) lines transformed with TaMIR5062 modified growth traits under drought and salt treatments; the lines overexpressing miRNA (i.e., Sen 1 and Sen 2) improved growth traits (i.e., biomass, leaf area, and root length) whereas those with knockdown (Anti 1) alleviated growth features compared with wild type. These results suggested the critical role of TaMIR5062 in improving plant drought and salt tolerance. In line with growth traits in stress-challenged lines, improved leaf water retention (i.e., promoted stomata closing, water losing rate, and osmolytes) and ROS-associated parameters (higher SOD, CAT, and POD activities, etc.) were shown in Sen 1 and Sen 2 under stress conditions. Antioxidant enzyme (AE) genes NtMnSOD1, NtCAT, and NtPOD9 encoding SOD, CAT, and POD, respectively, enhanced transcription in Sen 1 and downregulated expression in Anti 1 challenged with drought and salt stress. These results suggested the improved ROS homeostasis mediated by TaMIR5062 associates modified expression of distinct AE genes. Quantities of genes functional into categories “biological process”, “cellular component”, and “molecular function” contribute to TaMIR5062-mediated osmotic stress adaptation by regulating distinct biological pathways (i.e., protein folding) and metabolisms (i.e., photosynthesis and isoprenoid biosynthesis), which impact on plant osmotic-regulation, ROS homeostasis, and stress defensiveness underlying miRNA regulation. TaMIR5062 is a valuable target for molecular breeding of drought-tolerant crop cultivars.
Highly coherent frequency-entangled photons based on parametric instability in active fiber ring cavity
Authors: Gao Lei , Li Ziwei , Ran Hongqing , Cao Yulong , Wabnitz Stefan , Xiao Zinan , Dai Shiyun , Wu Qiang , Kong Lingdi , Huang Ligang , Huang Wei , Zhu Tao 
Published: December 2021

Abstract
Highly coherent frequency-entangled photons at telecom band are critical in quantum information protocols and quantum tele-communication. Photon pairs generated by spontaneous parametric down-conversion in nonlinear crystals or modulation instability in optical fibers exhibit random fluctuations. Here, we demonstrate highly stable frequency-entangled photons based on parametric instability in an active fiber ring cavity, where periodic modulation of dispersion excites parametric resonance, and the characteristic wave number is selected by the periodic modulation of resonator. Background-free autocorrelation of single-shot spectra reveals that spectra of parametric instability sidebands possess high coherence. The quantum properties are tested by the Hanbury Brown-Twiss measurement and Hong-Ou-Mandel interference. We conform the frequency-entanglement of two parametric instability sidebands by a spatial quantum beating with a fringe visibility of 97.9%. Our results prove that the parametric instability in active fiber cavity is effective to generate highly coherent frequency-entangled photon pairs, which would facilitate subsequent quantum applications.
Associations of Sleep Problems with Asthma and Allergic Rhinitis Among Chinese Preschoolers: A Cross-Sectional Study
Authors: Ma Ying , Tang Jie , Wen Yuqi , Hu Yan , Liang Jingjing , Jiang Lin , Xing Yanfei , Song Yanyan 
Published: December 2021

Abstract
Background

The aim of this study was to examine the associations of sleep problems with asthma and allergic rhinitis among Chinese preschoolers.
Methods

This cross-sectional survey was conducted in Guangzhou, China. Children aged 3-6 years were selected from 32 kindergartens in 7 regions. Asthma, allergic rhinitis and sleep problem were evaluated by a valid questionnaire. Binary logistic regression models were employed to estimate the odds ratios (OR) and 95% confidence intervals (CI) for asthma and allergic rhinitis according to short sleep duration, late bedtime and frequent nocturnal awaking.
Results

We included 4876 preschool children. Of these, 182 (3.7%) diagnosed as asthma, and 511 (10.5%) diagnosed as allergic rhinitis. Frequent nocturnal awakening was associated with asthma and allergic rhinitis, adjusted OR were 1.49(95% CI: 1.05~2.13) and 1.59(95%CI: 1.27-1.99), respectively. Further subgroup analysis showed the association of frequent nocturnal awakening with asthma differed by gender. No significant associations of short duration and late bedtime with asthma/ and allergic rhinitis were identified.
Conclusions

Our data suggested that frequent nocturnal awakening was associated with asthma and allergic rhinitis, and the association of frequent nocturnal awakening with asthma differed by gender. Further studies are warranted to address the causal relationship between nocturnal awaking and asthma and allergic rhinitis.
An Oxidative Stress Response Gene Model for the Prediction of Prognosis and Therapeutic Responses in Hepatocellular Carcinoma
Authors: HONG Junjie , Cai Xiujun 
Published: December 2021

Abstract
BackgroundOxidative stress response genes are critical for the development and progression of hepatocellular carcinoma (HCC). Still, the predictive value for prognosis and treatment response of oxidative stress response genes needs further elucidation. Methods﻿We obtained the transcriptomic data and corresponding clinicopathological information of HCC patients from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) databases. Oxidative stress response genes (OSRGs) were retrieved from the MSigDB database. LASSO Cox regression analysis was utilized to establish an integrated multi-gene signature in the TCGA cohort, and its prediction performance was validated in the ICGC cohort. The risk score of each patient ware determined by the multi-gene signature. The CIBERSORT algorithm was employed to evaluate the immune cell infiltration. Response rate to immune checkpoint inhibition (ICI) therapy was assessed using a TIDE platform. Tumor mutation burden was estimated using VarScan processed somatic mutation data. The drug activity data from the Cancer Genome Project and NCI-60 human cancer cell lines were used to predict sensitivity to chemotherapy. Results﻿The gene signature comprises G6PD, MT3, CBX2, CDKN2B, CCNA2, MAPT, EZH2, and SLC7A11. Patients with high risk scores had shorter overall survival. The risk score was identified as an independent prognostic marker. The immune cell infiltration patterns, response rates to immune checkpoint inhibition (ICI) therapy, and the estimated sensitivity of 89 chemotherapeutic drugs were associated with risk scores. Individual prognostic gene was also associated with the susceptibility of various FDA-approved drugs. Conclusion﻿Our study indicates that an integrated transcriptomic analysis may provide a reliable molecular model that better predicts diagnosis and forecasts the response of ICI therapy and chemotherapy.
Three-dimensional Deep-tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT)
Authors: Li Mucong , Beaumont Nathan , Ma Chenshuo , Rojas Juan , Vu Tri , Harlacher Max , O’Connell Graeme , Gessner Ryan , Kilian Hailey , Kasatkina Ludmila , Chen Yong , Huang Qiang , Shen Xiling , Lovell Jonathan , Verkhusha Vladislav , Czernuszewicz Tomek , Yao Junjie 
Published: December 2021

Abstract
Non-invasive small-animal imaging technologies, such as optical imaging, magnetic resonance imaging and x-ray computed tomography, have enabled researchers to study normal biological phenomena or disease progression in their native conditions. However, existing small-animal imaging technologies often lack either the penetration capability for interrogating deep tissues (e.g., optical microscopy), or the functional and molecular sensitivity for tracking specific activities (e.g., magnetic resonance imaging). To achieve functional and molecular imaging in deep tissues, we have developed an integrated photoacoustic, ultrasound and angiographic tomography (PAUSAT) system by seamlessly combining light and ultrasound in a non-invasive manner. PAUSAT can perform three imaging functions simultaneously with complementary contrast: high-frequency B-mode ultrasound imaging of tissue morphology, microbubble-enabled acoustic angiography of vasculature, and multi-spectral photoacoustic imaging of molecular probes. PAUSAT can provide three-dimensional (3D) multi-contrast images that are automatically co-registered, with high spatial resolutions at large depth. Using PAUSAT, we conducted proof-of-concept in vivo experiments on various small animal models: monitoring longitudinal development of placenta and embryo during mouse pregnancy, tracking biodistribution and metabolism of near-infrared organic dye on the whole-body scale, and detecting genetically-encoded breast tumor expressing photoswitchable phytochromes. These results have collectively demonstrated that PAUSAT has broad applicability in biomedical research, providing comprehensive structural, functional, and molecular imaging of small animal models.
Fractal, Diffraction-encoded Space-Division Multiplexing for FSO with Misalignment-Robust, Roaming Transceivers
Authors: Weng Xiaojing , Vuong Luat T. 
Published: December 2021

Abstract
With free space optical (FSO) communication systems, information is generally transmitted to minimize diffraction. Here, we demonstrate an alternate paradigm, "diffractal space-division multiplexing" (DSDM), in which the diffraction of fractals enable a wider cone for reception to support roaming transceiver. As a result, DSDM is robust to misalignment over longer-distance links. We examine the consequence of mid-field, non-far-field propagation and the result on kernel bit error rates. The sparse and redundant encoding of information in DSDM may be relevant to other FSO acquisition, pointing, and tracking.
Human pressure drives biodiversity–multifunctionality relationships in neotropical wetlands
Authors: Moi Dieison , Lansac-Tôha fernando , Romero Gustavo , Sobral-Souza Thadeu , Cardinale Bradley , Kratina Pavel , Perkins Daniel , de Mello Franco Teixeira , Jeppesen Erik , Heino Jani , Lansac-Tôha Fábio , Velho Luiz , Mormul Roger 
Published: December 2021

Abstract
Many studies have shown that biodiversity regulates a multitude of ecological functions that are needed to maintain the productivity and efficiency of a variety of types of ecosystems. What is not known is how human activities may change the ‘multifunctionality’ of ecosystems as they have both direct impacts on ecosystems and indirect effects on the biodiversity that serves to control ecological functions. Using a database on hundreds of lakes spanning four large neotropical wetlands, we demonstrate that species richness and the functional diversity of fish, macrophytes, microcrustaceans, rotifers, protists, and phytoplankton are positively associated with ecosystem multifunctionality, including nutrient concentrations, standing biomass, and ecosystem metabolism. However, we also found that the relationship between biodiversity and multifunctionality is weakened by human pressures and that part of this impact occurs through changes in biodiversity. Our results suggest that human activities may break down the biological controls needed to maintain the suite of ecosystem functions that sustain wetlands.
Effect of Scanning Mode on Temperature Field and Interface Morphology of Laser Joining between CFRTP and TC4 Titanium Alloy
Authors: Chen Weiwen , Liu Xiqin , Bu Hengchang , Wang Feiyun , Luo Jiebang , Zhan Xiaohong 
Published: December 2021

Abstract
Hybrid components composed of CFRTP (Carbon Fiber Reinforced Thermoplastic Polymer) and TC4 titanium alloy are increasingly applied in the aerospace field. The scanning mode has a significant influence on the quality of laser joining joint between CFRTP and TC4 titanium alloy. Therefore, the laser joining between TC4 titanium alloy with surface microgrooves and CFRTP has been implemented under oscillating laser joining mode and linear laser joining mode respectively in the present research. The temperature distribution is qualitatively explored based on the established mathematical model of laser joining between CFRTP and TC4 titanium alloy. The interface morphology and the joining strength of CFRTP/TC4 titanium alloy lap joints under oscillating laser joining and linear laser joining are compared. The results indicate that the simulated temperature distribution shows good agreement with the experimental result. Compared with linear laser joining, the oscillating laser joining weakens the heat concentration and creates a heating zone with larger area and more uniform temperature distribution. The interface morphology of laser joining CFRTP/TC4 titanium alloy joints with better resin filling and fewer bubble defects is obtained by oscillating laser joining due to the temperature variation of the form of unequal amplitude oscillations, whereas there are a large number of large-size bubbles in the filling resin and small-sized fusion gaps distributed at the interface with the linear laser scanning mode. By adopting the joining method with oscillating laser scanning mode, higher quality joints can be obtained.
Particle Simulation of The Strong Magnetic Field Effect On Dust Particle Charging Process
Authors: Davari H. , Farokhi B. , Asgarian M. Ali 
Published: December 2021

Abstract
A particle-in-cell simulation is modeled and run on a dusty plasma to determine the effect of the magnetic field on the process dust-particle charging through electron-ion plasma. The electric field is solved through the Poisson equation, and the electron-neutral elastic scattering, excitation, and ionization processes are modeled through Monte Carlo collision method. The effects obderved from the initial density of the plasma, the initial temperature of the electrons, and the changing magnetic field are included in this simulation model. In the dust particle charging process, saturation time and saturation charge are compared. An increase in the magnetic field cannot reduce time to reach the saturation state. Determinig the magnetic field boundaries which depend on the physical properties of the plasma, which can be contributive in some areas of dusty(complex) plasma. The applications of the results obtaind here for fusion plasma conditions and space and laboratory plasmas are discussed. The results here can be applied in future simulation models with a focus on the dust particle movement and their effect on plasma, leading to the modeling of different astrophysical plasmas thorough laboratory experiments.
Study on the Influence of Rotational Speed and Pad Temperature of Pumped-Storage Set on Oil Mist Leakage of Thrust Bearing
Authors: Sun Jie , Zhang Yuquan , Zheng Yuan , Ge Xinfeng , Hong Yunlai , Fernandez-Rodriguez E. 
Published: December 2021

Abstract
The accumulation of oil mist in the thrust bearings compromises the safety of the unit and prompts financial and environmental losses. To discuss the strategies for limiting its impact, the model uses the VOF approach to calculate the air-oil-oil mist three-phase flow, combined with the Lee model, to solve the evaporation and mass transfer process between the oil and oil mist. Our attention is drawn into the influence of unit speed and pad temperature, on the occurrence of external and internal leaks from the oil tank. A comparison of cases is made with five unit speeds (100 to 500 rpm) at constant temperature (60°C), and with one unit speed (500 rpm) at different temperatures (56°C,60°C,62°C). With constant speed, the rise of pad temperature promotes the evaporation of the lubricating oil in an uneven manner, augmenting the occurrence of oil leaks. From findings, the increase of speed reduces the pressure change rate at the wall of the inner tank (external oil leaks) by 5.95 %, and of the oil slinger (internal leaks), by 44.64%. The present results might help to suggest courses of action to reduce the oil mist leakage.
Knowledge, Attitudes, Practices and Perceived Barriers Among Mental Health Professionals in Sudanese Psychiatric Hospitals Regarding COVID-19 Outbreak
Authors: Elhashimi Fajr , Ibrahim Mohammed , Taha Bhaga 
Published: December 2021

Abstract
Background: Since March 11, 2020, COVID-19 has been declared as a pandemic by WHO, it has caused substantial morbidity and mortality globally thus knowledge, attitude, and practice (KAP) related to the COVID-19 outbreak are one of the priorities in such a pandemic. KAP in a non-COVID facility, such as a psychiatric institution needs to be addressed. Aims: To assess mental health professionals knowledge, attitude, and practice working Sudanese in psychiatric facilitatesMethods: A cross-sectional self-administered questionnaire targeting the whole population of mental health professionals(MHPs) which is 141, was conducted at three psychiatric facilities in Khartoum state , Sudan. 89 agreed to participate in the study consisting of psychiatrists, psychologists, social workers, and psychiatric nurses staff participated in the study. Results: 70.8% of MHP’s in this study had adequate knowledge of COVID-19 symptomatology, transmission, management, and preventive measures. 44.9% were not willing to take COVID19 vaccine. Good practices like maintaining quarantine during outbreak and wearing a medical mask were observed. The significant difference found in Spearman’s correlation was between knowledge and attitude which was negative correlation. Findings showed that MHPs (37.1%,N=33) had positive Practice,(57.3%,N=51) had positive attitude regarding COVID-19. MHPs perceived that lack of an official specialized in infection control ,overcrowding in psychiatric patients’ room and Lack of policy procedures of infection control practice were the major barriers to infection control. Inferential analysis indicated that Social workers showing higher knowledge (mean=57.95) than other MHPs (P=0.009) and psychiatrist showed higher attitude (62.63). Conclusions: This study found that MHPs in Sudan have good knowledge, and suggest a special need to scale up facilities’ infection control materials to meet the required good practice.
Probiotic Bifidobacterium Isolated from Chicken Intestine with PVP Showed Synergistic Effects on Reduction of AFB1
Authors: Aalipanah sorour , Fazeli Mohammad Reza , Sepahi Abbas Akhavan , Shariatmadari Farid 
Published: December 2021

Abstract
Food contamination with aflatoxin is one of the most important concerns of health professionals. One of the best ways to reduce aflatoxin content in food is to use probiotics. Therefore, this study was performed to isolate Bifidobacterium from the chick's intestine and its probiotic activities and also its application with Polyvinylpyrrolidone (PVP) to reduce aflatoxin B1 (AFB1) toxin in the medium were investigated. Samples were isolated from the chicken intestine. After preparing the samples, Bifidobacterium was isolated and identified using biochemical and molecular methods. To measure probiotic activities, pH, bile, and salt tolerance tests were used. Then, the antimicrobial activity of isolate against gastrointestinal pathogens and the antibiotic susceptibility test were done. Then, the effect of selected isolate and PVP on reducing AFB1 in the medium was studied using ELISA and HPLC. Biochemical and molecular evaluations indicated isolation of B. bifidum strain from chicken intestine. The selected strain showed antimicrobial activities on S. enterica, E. coli, and P. vulgaricus and was found to be resistant against Amikacin, Ampicillin, Erythromycin, and Ceftazidine antibiotics. The selected strain showed the ability to reduce the concentration of AFB1 in the medium (50% reduction) and when used in combination with PVP showed the synergistic effects in reducing the concentration of AFB1 from the medium (up to 90%). In conclusion, it was found that selected B. bifidum strain together with PVP could have synergistic effects in reducing AFB1 toxin in medium up to 90%.
A Deep Learning Nomogram for the Prediction of Early Recurrence in Hepatocellular Carcinoma After Curative Surgery
Authors: Yan Meng , Zhang Xiao , Zhang Bin , Geng Zhijun , Xie Chuanmiao , Yang Wei , Zhang Shuixing , Qi Zhendong , Lin Ting , Ke Qiying , Li Xinming , Wang Shutong , Quan Xianyue 
Published: December 2021

Abstract
Purpose: The accurate prediction of post-hepatectomy early recurrence in patients with hepatocellular carcinoma (HCC) is crucial for decision-making regarding postoperative adjuvant treatment and monitoring. We aimed to develop and validate a deep-learning (DL) nomogram based on MRI for predicting early recurrence in HCC after curative resection. Methods: We retrospectively included 285 HCC patients who underwent Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced MRI within one month before curative resection. Deep features were extracted from images of the arterial phase (AP), portal venous phase (PVP), and hepatobiliary phase (HBP) using VGGNet-19. Pearson’s correlation was firstly used to exclude redundant features. Three feature selection methods and five classification methods were combined to construct the DL signature. Univariate and multivariate logistic regression analyses were used to identify the independent risk factors for the early recurrence, which were incorporated into the DL nomogram. Results: Microvascular invasion (P = 0.039), tumor number (P = 0.001), and three-phase-based DL signature (P<0.0001) were independent risk factors for early recurrence. The DL nomogram integrating the DL signature and clinical risk factors outperformed the clinical nomogram which combined clinical risk factors, in the training set (AUC: 0.949 vs. 0.751; P<0.0001) and validation set (AUC: 0.908 vs. 0.712; P = 0.002). Excellent calibration was achieved for the DL nomogram in both training and validation sets. Decision curve analysis confirmed the clinical usefulness of the DL nomogram. Conclusions: The proposed DL nomogram was superior to the traditional clinical nomogram in predicting early recurrence for HCC patients after curative resection.