bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis
Authors: Ye Tao , Feng Shao , Zheng Liu , Ming Cai , Futao Meng , Qiang Huang 
Published: August 2021

Abstract
Chronic pancreatitis (CP) is a precancerous condition associated with pancreatic ductal adenocarcinoma (PDAC), but its evolutionary mechanism is unclear. Pancreatic stellate cells (PSCs) are closely related to the occurrence and development of CP and PDAC, but it is not clear whether PSCs play a key role in this "inflammation-cancer transition". Our research found that co-culture with activated PSCs promoted the proliferation, migration and invasion of normal pancreatic duct epithelial cells and pancreatic cancer cells. At the same time, activated PSCs had a significant effect on the expression of the glycolysis markers (pyruvate kinase M2, lactate dehydrogenase A, glucose transporter 1, hexokinase-II and monocarboxylate transporter 4; PKM2, LDHA, GLUT1, HK2 and MCT4) in normal pancreatic duct epithelial cells and pancreatic cancer cells and increased lactic acid production and glucose consumption in these two cells. In vivo experiments showed that the expression of the glycolysis markers in pancreatic duct epithelial cells and the marker protein (α-SMA) of activated PSCs in the pancreatic duct peripancreatic interstitium were higher in pancreatic cancer tissues and chronic pancreatitis tissues than in normal pancreatic tissues in both animals and humans. In addition, analysis of human tissue specimens showed that there is a correlation between the expression of glycolysis markers and α-SMA. These findings indicate that activated PSCs play an important role in the development and progression of chronic pancreatitis into pancreatic cancer by regulating and promoting aerobic glycolysis. Our research provides a new theoretical basis for further understanding the mechanism of CP malignancy and the selection of targets for reversing CP malignancy.
A Radiomics Nomogram for Non-Invasive Prediction of Progression-Free Survival in Esophageal Squamous Cell Carcinoma
Authors: Yan Ting , liu lili , Peng Meilan , Yan Zhenpeng , Wang Qingyu , Zhang Shan , Wang Lu , Zhuang Xiaofei , Liu Huijuan , Ma Yanchun , Wang Bin , Cui Yongping 
Published: August 2021

Abstract
Objectives: To construct a prognostic model for preoperative prediction based on computed tomography (CT) images of esophageal squamous cell carcinoma (ESCC). Methods: Radiomics signature was constructed using the least absolute shrinkage and selection operator (LASSO) with high throughput radiomics features that extracted from the CT images of 272 patients (204 in training and 68 in validation cohort), who were pathologically confirmed ESCC. Multivariable logistic regression was adopted to build the radiomics signature and another predictive nomogram model, which was composed with radiomics signature, traditional TNM stage and clinical features. Then its performance was assessed by the calibration and decision curve analysis (DCA). Results: 16 radiomics features were selected from 954 to build a radiomics signature，which were significantly associated with progression-free survival (PFS) (p<0.001). The area under the curve (AUC) of performance was 0.891 (95% CI: 0.845-0.936) for training cohort and 0.706 (95% CI: 0.583-0.829) for validation cohort. The radscore of signatures’ combination showed significant discrimination for survival status in both two cohort. Kaplan-Meier survival curve further confirmed the radscore has a better prognostic performance in training cohort. Radiomics nomogram combined radscore with TNM staging showed significant improvement over TNM staging alone in training cohort (C-index, 0.802 vs 0.628; p<0.05), and it is the same with clinical data (C-index, 0.798 vs 0.660; p<0.05). Findings were confirmed in the validation cohort. DCA showed CT-based radiomics model will receive benefit when the threshold probability was between 0 and 0.9. Heat maps revealed associations between radiomics features and tumor stages.Conclusions: Multiparametric CT-based radiomics nomograms provided improved prognostic ability in ESCC.
SSE Path Routing Protocol for WSN IOT Using Fuzzy Mean Weighted Code and Enthalpy ANN-GOA
Authors: Rani Poonam , Sharma Avinash 
Published: August 2021

Abstract
Wireless Sensor Networks (WSNs) play a key role in the Internet of Things (IoT) by delivering cost-effective solutions for specific IoT applications. The amount of energy used to transport data from a sensor node to its destination is a significant consideration when developing wireless sensor network routing strategies. A variety of routing protocols are utilised in WSN to improve network performance. When a node's mode transitions from active to sleep, the efficiency declines because data packets must wait at the starting point where they were transmitted, which increases packets' waiting time and end-to-end latency, resulting in an increase in energy consumption. This study proposes the SSE Path routing protocol as a Scalable, Secure, and Energy Efficient Routing Protocol for WSN IoT. Our proposed protocol, first selecting the nodes using a single-hop clustering algorithm. Second, FMWC (Fuzzy Mean Weighted Code) based packet coded operation for the security of packet. Finally, Scalable Enthalpy Artificial Neural-Network Based Grasshopper Optimization Algorithm (EANN-GOA) algorithm for selecting the congestion-free path for transit the data packets. The experimental results revelations the dominance of presented protocol comparing with the existing protocols.
Metabolic remodeling during murine early embryo development
Authors: Zhao Jing , Yao Ke , Yu Hua , Zhang Ling , Xu Yuyan , Hu Zeping , Zhang Jin , Chen Lang , Sun Zhen , Zhu Yuqing , Zhang Chen , Qian Yuli , Ji Shuyan , Zhang Min , Chen Jie , Correia Cristina , Weiskittel Taylor , Lin Da-Wei , Chandrasekaran Sriram , Fu Xudong , Zhang Dan , Fan Heng-Yu , Xie Wei , Li Hu , Hu Zeping , Zhang Jin 
Published: August 2021

Abstract

During early mammalian embryogenesis, dynamic changes in cell growth and proliferation are tightly linked to the underlying genetic and metabolic regulation. However, our understanding of metabolic reprogramming and its impact on epigenetic regulation in early embryo development remains elusive 1. Here, we profiled metabolomes of embryos from the 2-cell and blastocyst stages, and their in vitro counterpart 2-cell like cells and ES cells, and reconstructed their metabolic landscape through the transition from totipotency to pluripotency. Our integrated metabolomics and transcriptomics analysis showed that 2-cell embryos favor methionine, polyamine and glutathione metabolism and stay in a more reductive state, whereas blastocyst embryos have higher mitochondrial TCA cycle metabolites and are in a more oxidative state. Moreover, we identify a reciprocal relationship between α-ketoglutarate (α-KG) and the competitive inhibitor of α-KG-dependent dioxygenases L-2-hydroxyglutarate (2-HG)2, namely, higher L-2-HG in the 2-cell embryos inherited from oocytes and 1-cell zygotes, and higher α-KG in the blastocyst. Supplementing 2-HG or knocking down L2hgdh, a gene encoding the 2-HG consuming enzyme L-2-hydroxyglutarate dehydrogenase 3 impeded erasure of global histone methylation markers 4–6. Together, our data demonstrate dynamic and interconnected metabolic, transcriptional and epigenetic network remodeling during murine early embryo development.
NLRP3 Promotes Immune Escape by Regulating Immune Checkpoints: A Pan-cancer Analysis
Authors: Ding Yue , Yan Yilin , Dong Yihui , Xu Jingyuan , Su Wei , Yang Zhou , Zou Qi , Yang Xiaoping 
Published: August 2021

Abstract
NLRP3 plays a pathogenic role in tumorigenesis by regulating innate and acquired immunity, apoptosis, differentiation, and intestinal microbes in tumors. In different tumors, NLRP3 plays different roles, and its mechanism is complex. Our research aimed to comprehensively investigated the role of NLRP3 in pan-cancers based on multi-omics data in the TCGA database. We found the expression of NLRP3 was changed in tumors compared with paired non-tumor specimens. Most types of tumors showed increased expression of NLRP3. Among them, the overexpressed NLRP3 in liver hepatocellular carcinoma (LIHC) and ovarian cancer (OV) indicated worse overall survival (OS). Further analysis also confirmed overexpressed NLRP3 in colon cancer (COAD) indicated a high probability of microsatellite instability (MSI) and low tumor mutational burden (TMB), which indicated a better response to immune checkpoint inhibitors (ICIs). We also analyzed the association between NLRP3, immune infiltration, and immune checkpoints. Interestingly, overexpression of NLRP3 was closely related to high infiltration of immune cells (T cells, B cells, etc.) and overexpressed immune checkpoints (PD-1, PD-L1, LAG3, etc.). These results demonstrated NLRP3 promoted immune escape in cancers. Finally, we investigated the expression of various immune checkpoints by treating NLRP3 inhibitor MCC950 during the co-culture of peripheral blood mononuclear cells (PBMC) and LIHC cell line Hep3B. We found MCC950 significantly repressed the expression of PD-L1 and LAG3, and promoted the apoptosis rate of Hep3B. In conclusion, our research comprehensive demonstrated the role of NLRP3 in pan-cancer, especially in LIHC. We confirmed inhibition of NLRP3 promoting the immune killing effect to cancer cells by repressing the expression of immune checkpoints.
Nucleotide Variation In The Phytoene Synthase (ClPsy1) Gene Contributes To Golden Flesh In Watermelon (Citrullus Lanatus L.)
Authors: Liu Shi , Gao Zhongqi , Luan Feishi , Wang Xuezheng , Dai Zuyun , Yang Zhongzhou , Zhang Qian 
Published: August 2021

Abstract
Vitamin A deficiency is a worldwide public nutrition problem, and β-carotene is the precursor for vitamin A synthesis. Watermelon with golden flesh (gf, due to accumulated abundance of β-carotene) is an important germplasm resource. In this study, a genetic analysis of gf segregating populations indicated that gf was controlled by a single recessive gene. BSA-seq and an initial linkage analysis placed the gf locus in a 290-Kb region on watermelon chromosome 1. Further fine mapping in a large population with over 1,000 F2 plants narrowed this region to 39.08 Kb harboring two genes, Cla97C01G008760 and Cla97C01G008770, which encode phytoene synthase (ClPsy1) and GATA zinc finger domain-containing protein, respectively. Gene sequence alignment and expression analysis between parental lines revealed Cla97C01G008760 as the best possible candidate gene for gf trait. Nonsynonymous SNP mutations in the first exon of ClPsy1 between parental lines cosegregated with the gf trait only among individuals in the genetic population but were not related to flesh color in natural watermelon panels. Promoter sequence analysis of 26 watermelon accessions revealed two SNPs in the cis-acting element sequences corresponding to MYB and MYC2 transcription factors. RNA-seq data and qRT-PCR verification showed that two MYBs and one MYC2 exhibited expression trends similar as ClPsy1 in the parental lines, which may thus play roles in the regulation of ClPsy1 expression. Our research findings indicate that the gf trait is determined not only by ClPsy1 but also by ClLCYB, ClCRTISO and ClNCED7, which play important roles in β-carotene accumulation in watermelon flesh.
Agreement Between Rapid Hepatitis B Surface Antigen Test Kit and ELISA for the Diagnosis of Hepatitis B Virus Infection in Gondar, North West Ethiopia.
Authors: Lemma Mulualem , Tsegay Gueshay , Adane Kasaw 
Published: August 2021

Abstract
Background: Hepatitis B Virus is one of the major causes of global public health problems. Diagnosis of HBV is done by using ELISA and immune-chromatographic assays for detecting different serologic markers. Hepatitis B surface antigen (HBsAg) is the most frequently used serological marker employed to diagnosis HBV using Rapid Diagnostic Test (RDT) and Enzyme Linked Immunosorbent Assay (ELISA) methods. The RDT method is the most commonly used diagnostic tool in Ethiopia as well as in most African countries. Therefore assessing and knowing the diagnostic performance of the RDT is important. Method: A prospective cross sectional study was done at University of Gondar comprehensive specialized Hospital from Feb, 2017- May, 2017. Ethical clearance was obtained from School of Biomedical and Laboratory Sciences Research Ethics Committee. Permission was obtained from Blood Bank office to use blood sample collected from voluntary blood donors. HBsAg was diagnosed using RDT and ELISA methods. ELISA considered as gold-standard to the performance of RDT. Data were entered into statistical package for social sciences (SPSS) version 20 software and analysis was done by using SPSS and for sensitivity, specificity, predictive value, and likelihood ratio Medi calc software. The agreement of the methods presented assessed using kappa value. Result: Each 161 specimen was diagnosed using both ELISA (ADVANCED®) and RDT (Ecotest®). A total ELISA confirmed 41 positive specimens and 120 ELISA confirmed negative specimens were re-analyzed using RDT. The results of RDT showed only 35 positives from the total tested specimens including 29 which already positive by ELISA and RDT showing a sensitivity of 70.7% and specificity 95% with its kappa value 0.69 indicating a good agreement with ELISA.Conclusion and recommendation: Rapid Ecotest® test of HBsAg has good agreement with ELISA but low sensitivity and high false negative value, so there should be an algorithm for HBV diagnostic methods when we used a rapid test kits.
TMT-Based Proteomic Profiling Reveals the Molecular Mechanism in the Cold Tolerance of Rhododendron Aureum Georgi.
Authors: Zhou Xiaofu , Cao Kun , Zhang Ziyao , Tan Yun , Fan Hang , Xu Hongwei 
Published: August 2021

Abstract
Background: Cold stress is one of the important factors that restrict plant growth and regional distribution. Under cold acclimation conditions, plants growing in temperate zones reprogram the gene expression in the cells, making the plants better cope with the coming cold stress. However, under natural environmental conditions, the climate is complex and changeable. The sudden large temperature drop will bring serious disasters to plants. Rhododendron aureum Georgi, as an evergreen plant growing in high altitude areas of Changbai Mountain, the harsh ecological environment may endow it with different cold tolerance characteristics. Results: In this study, the proteomic difference between samples under control and cold stress were compared pairwise. A total of 360 DAPs were identified, of which 175 were down-regulated and 185 were up-regulated when comparing these two sets of data. The high cold tolerance of Rhododendron plants can be attributed to: accumulation of chaperone proteins; the up-regulation of components related to translation; enhancement of catabolism and reduction in anabolism, provide energy for plants and fight against cold stress; enhanced cellular antioxidant capacity; modification of components in cell wall, membrane, and cytoskeleton. Conclusion: These results provide an in-depth understanding of the cold tolerance mechanism of Rhododendron. The identified genes and metabolic pathways provide a certain reference for the genetic improvement of plant cold tolerance.
Mothers’ Experiences of Wellbeing and Coping While Living with Rheumatoid Arthritis: A Qualitative Study
Authors: Parton Chloe , Ussher Jane M. , Perz Janette 
Published: August 2021

Abstract
Background

Rheumatoid arthritis (RA) can result in difficulties for mothers when undertaking daily care activities and increased psychological distress. However, few studies have examined how women with RA subjectively experience coping and wellbeing as part of their motherhood.
Methods

Twenty mothers with a diagnosis of RA and a dependent child (18 years or younger) who were living in Australia took part in a semi-structured interview between June and November 2017. Purposive sampling was undertaken to include participants across degree of current RA severity, number and age of children, and having received a diagnosis before or after a first child to take account of variability across these experiences. A qualitative thematic analysis was conducted on the interview transcripts.
Results

The following themes were identified: ‘Burden and complexity in the mothering role’, ‘Losing control: Women’s experiences of distress’, and ‘Adjusting and letting go: Women’s experiences of wellbeing’. Experiences of distress, including feelings of failure, were associated with accounts of a loss of control over mothering practices among women, regardless of child age. In contrast, accounts of adjusting mothering practices and relinquishing control were associated with reports of enhanced wellbeing. In addition, some mothers reported greater ease due to increased independence of older children. The absence of social support exacerbated burden and distress in the women’s accounts, while the availability of support alleviated burden and was associated with reports of wellbeing.
Conclusion

Health professionals and services can provide support to mothers with RA by addressing feelings of failure, acknowledging strategies of adjustment and letting go, and encouraging access to social support.
Application of Neck Ultrasound in the Diagnosis of Sarcoidosis
Authors: Shen Mengjun , Zhou Ying , Gu Weiqing , Yin Chengsheng , Wang Yin , Zhang Yuan 
Published: August 2021

Abstract
Objective: To explore the significance of neck ultrasound (NUS) combined with contrast-enhanced ultrasound (CEUS) in the diagnosis of sarcoidosis.Methods: 88 patients with evidence of intrathoracic lymphadenopathy and suspected sarcoidosis with enlarged cervical lymph nodes underwent NUS, CEUS, fine-needle aspiration (FNA) and core needle biopsy (CNB) when technically feasible were retrospectively analyzed in this study. Seven characteristics such as enhanced mode (EM), resolution time (RT), Color Doppler Flow Imaging (CDFI) , fading time (FT), peaking state-uniformity (PTSU), strengthen the area (STA) and symmetry were considered to perform the logistic regression model.Results: Of 88 patients included in this study, sarcoidosis was accounted in 20 cases, tuberculosis in 23 cases, malignancy in 22 cases and inflammatory lymph node in 23 cases. There were statistically significant differences in symmetry, lymphatic hilum, homogeneity, CDFI pattern and elasticity score between the sarcoidosis and non-sarcoidosis groups via NUS. Similarly, we also acknowledged a statistically significant differences in enhanced mode, homogeneity, presence or absence of necrosis between the sarcoidosis and non-sarcoidosis groups via CEUS to further group the non-sarcoidosis into tuberculosis, malignancy or inflammatory disorder. The percentage correction of prediction was 90% (18/20).Conclusion：NUS combined with CEUS has characteristic features in sarcoidosis with cervical lymph node involvement, which is helpful for its diagnosis and differential diagnosis. The binary classification model of NUS combined with CEUS features can help differentiate sarcoidosis from non-sarcoidosis groups.
Modeling and Finite-Time Sliding Model Control of a Lower Limb Exoskeleton for Use in Rehabilitation
Authors: Tople Uddesh Kishor , Khandare Amrapali Anandkumar , Itankar Atharv Dipak , Dogga Satya Kartheek 
Published: August 2021

Abstract
Exoskeleton systems in recent years has become a prime choice technology due to the various possibilities it can deliver. These possibilities comprise the assisting and rehabilitative techniques designed for disabled and elderly people, so that they can regain control of their limbs and in addition to this also to augment and boost the abilities of able-bodied persons during heavy work-load conditions. Many works are reported on the modeling and control of a exoskeleton robot, but very few paper discuss the complete derivation of the model of the system. Here, first the dynamic model of a physical system used as lower limb exoskeleton robot is obtained. Secondly the analysis of the system done that is derived through dynamic modelling of a 3-link robotic manipulator using Euler-Lagrange approach and validation of the corresponding model in simulation. Further, design of a finite-time SMC for desired trajectory tracking of the system is implemented. The dynamic model of the 3-link system and its control using finite-time sliding mode control are validated in MATLAB simulation environment.
IRF2 Destabilizes Oncogenic KPNA2 to Modulate the Tumorigenesis of Osteosarcoma via Regulating NF-κB/p65
Authors: Xia Shuchi , Ma Yiqun 
Published: August 2021

Abstract
Background: Osteosarcomas (OS) are the most frequent primary malignant bone tumor. Emerging evidence revealed that karyopherin alpha 2 (KPNA2) was strongly associated with the tumorigenesis and development of numerous human cancers. The aim of the present study was to investigate the expression pattern, biological functions and underlying mechanism of KPNA2 in OS. Methods: Bioinformatics TFBIND online was applied to forecast the transcription factor (TF) binding sites in the promoter region of KPNA2. The expression profile of KPNA2 in OS tissues were firstly assessed using TARGET dataset. The expression of KPNA2 in clinical OS samples and normal human adjacent samples were analyzed by RT-qPCR and western blot. CCK8, colony formation, wound-healing, and Transwell assays were used to assess cell viability, proliferation and migration in vitro, and in vivo experiments were performed to explore the effects of KPNA2 and interferon regulatory factor-2 (IRF2) on tumor growth. In addition, the correlation between IRF2 and KPNA2, and their roles on the NF-κB/p65 was investigated using chromatin immunoprecipitation (ChIP), RT-qPCR, western blot and dual-luciferase assays. Results: KPNA2 was obviously upregulated while IRF2 was significantly decreased in OS tissues and cell lines, as well as they were negatively correlated with each other. KPNA2 knockdown remarkably suppressed OS cell growth, migration, invasion in vitro and tumor growth in vivo, while IRF2 knockdown exerts an opposing effect. IRF2 binds to KPNA2 promoter to modulate the tumorigenic malignant phenotypes of OS via regulating NF-κB/p65 signaling. Conclusion: The present study demonstrated that KPNA2 performed the oncogenic function, possibly regulating tumorigenesis through NF-κB/p65 signaling pathway. Importantly, IRF2 was confirmed to serve a potential upstream TF of KPNA2 involving in the regulation of NF-κB/p65 pathway in OS.