bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding and working with different worldviews to co-design cultural security in clinical mental health settings to engage with Aboriginal and Torres Strait Islander clients
Authors: Wright Michael , Lin Ashleigh , O’Connell Margaret , Bullen Jonathan , Flavell Helen 
Published: October 2021

Abstract

Background:
Creating the conditions for meaningful relationships is essential to understanding Aboriginal worldviews and co-designing ways of working to achieve better health outcomes. Non-Aboriginal health professionals struggle to recognise the importance of social relationships to Aboriginal peoples and tensions emerge due to these different worldviews informed by different ontologies and epistemologies. This is more so in clinical settings where training and models of care are often inadequate for working with Aboriginal people. The impact of different understandings of relationships on the provision of health services to Aboriginal peoples remains under-researched. There is a critical need to reassess the way clinicians are supported by their organisations to engage with Aboriginal clients in competent and meaningfully ways.

Methods:
The paper provides key insights into an Aboriginal-led participatory action research project and the work of Aboriginal Elder co-researchers with non-Aboriginal mainstream service staff to better understand the importance of social relationships from an Aboriginal worldview. The paper critically engages literature on clinical service provision for Aboriginal peoples, along with an examination of the Australian Psychological Society Code of Conduct, to explore the tensions between professional training and the need to build relationships with Aboriginal clients.

Findings:
Through the Elders, non-Aboriginal service staff have expanded their understanding of Aboriginal culture, kinship and the importance of country to Aboriginal wellbeing. The Elders mentored staff to unpack the tensions between worldviews in clinical settings. The research resulted in a co-designed culturally safe framework for non-Aboriginal practitioners, which is building confidence, capacity and competence to work in partnership with Aboriginal peoples. The framework emphasis the need for culturally safe models of care. The Elders have supported non-Aboriginal staff to sit between the two worldviews to develop ways to work with Aboriginal clients and shift mainstream models of mental health care to improve the wellbeing of Aboriginal people.
Chemical Nature of Hydrothermal Fluids Generated by Serpentinization and Carbonation of Komatiite: Implications for H2‐Rich Hydrothermal System and Ocean Chemistry in the Early Earth
Authors: Ueda Hisahiro , Shibuya Takazo , Sawaki Yusuke , Shozugawa Katsumi , Makabe Akiko , Takai Ken 
Published: October 2021

AbstractH2‐rich ultramafic‐hosted hydrothermal systems are considered to be important places for the origin and early evolution of life. In this study, we conducted two hydrothermal serpentinization/carbonation experiments involving synthetic komatiite under CO2‐rich conditions to further understand the chemical nature of hydrothermal fluids in the komatiite‐hosted seafloor hydrothermal systems in the early Earth. The H2 concentration in fluid at 300°C is one order of magnitude lower than that under CO2‐free conditions, which revealed that the carbonation of komatiites suppressed H2 generation under CO2‐rich conditions. The steady‐state H2 concentrations in the fluid increased with increasing temperature, while the Fe content of carbonate minerals in the run products increased with decreasing temperature. This correlation suggested that Fe incorporation into the carbonate minerals would limit the H2 generation during the serpentinization of komatiites at each temperature. In addition, the pH of the fluid also depended on temperature. High‐temperature (>300°C) fluids became alkaline, whereas low‐temperature fluids became acidic. Thermodynamic calculations show that the acidic fluids were attributed to high water/rock ratio during the hydrothermal reactions. Consequently, H2‐poor and acidic fluids were likely generated at low temperatures. In contrast, the H2‐rich and alkaline hydrothermal fluids were generated at temperatures above 300°C, which suggests that high‐temperature seafloor hydrothermal systems may be more favorable than lower‐temperature systems for the emergence and early evolution of life in the Hadean ocean in terms of the formation of energy and electrochemical potentials between hydrothermal fluids and ambient seawater.
Global Impact of Lightning‐Produced Oxidants
Authors: Mao Jingqiu , Zhao Tianlang , Keller Christoph A. , Wang Xuan , McFarland Patrick J. , Jenkins Jena M. , Brune William H. 
Published: October 2021

AbstractLightning plays a major role in tropospheric oxidation, and its role on modulating tropospheric chemistry was thought to be emissions of nitrogen oxides (NOx). Recent field and laboratory measurements demonstrate that lightning generates extremely large amounts of oxidants, including hydrogen oxides (HOx) and O3. Here, we implement these lightning‐produced oxidants in a global chemical transport model to examine its global impact on tropospheric composition. We find that lightning‐produced oxidants can increase global mass weighted OH by 0.3%–10% and affect CO, O3, and reactive nitrogen substantially, depending on the emission strength of oxidants from lightning. Our work highlights the importance and uncertainties of lightning‐produced oxidants, as well as the need for rethinking the role of lightning in tropospheric oxidation chemistry.
Topside Measurements at Jicamarca During the 2019–2020 Deep Solar Minimum
Authors: Derghazarian S. , Hysell D. L. , Varney R. H. 
Published: October 2021

AbstractWe present measurements of the equatorial topside ionosphere above Jicamarca made during extremely low solar flux conditions during the deep solar minimum of 2019–2020. Measurements were made in October, 2019, February, 2020, and September, 2020. The main features observed are a large and extended decrease in noontime temperatures unlike that seen in studies at moderate solar flux levels, predawn ionospheric heating as early as 0300 LT, large day‐to‐day variability in the O+/H+ transition height, and negligible helium ion concentration at all altitudes. Data from the Ion Velocity Meter (IVM) instrument onboard the Ionospheric Connection Explorer (ICON) and the Topside Ionospheric Plasma Monitor (SSIES) onboard the Defense Meteorological Satellite Program (DMSP) satellites are used to assess agreement with ISR data and assist with the analysis of the predawn heating phenomena. We also analyze the data in light of the SAMI2‐PE model which shows less agreement with the data than at higher solar flux. The main areas of discrepancy with the data are outlined, such as the absence of significant predawn heating, less pronounced decreases in noontime temperatures, and much higher O+ fractions at high altitudes, particularly in September. Finally, a sensitivity analysis of the model to various forcing agents such as neutral winds, plasma drifts, solar flux, and heat flow is performed. A discussion is presented on bridging the discrepancies in future model runs. Novel techniques of clutter removal and noise power bias correction are introduced and described in the appendices.
Probabilistic Machine Learning Estimation of Ocean Mixed Layer Depth From Dense Satellite and Sparse In Situ Observations
Authors: Foster Dallas , Gagne David John , Whitt Daniel B. 
Published: October 2021

AbstractThe ocean mixed layer plays an important role in the coupling between the upper ocean and atmosphere across a wide range of time scales. Estimation of the variability of the ocean mixed layer is therefore important for atmosphere‐ocean prediction and analysis. The increasing coverage of in situ Argo profile data allows for an increasingly accurate analysis of the mixed layer depth (MLD) variability associated with deviations from the seasonal climatology. However, sampling rates are not sufficient to fully resolve subseasonal ( day) MLD variability. Yet, many multivariate observations‐based analyses include implicit modeled subseasonal MLD variability. One analysis method is optimal interpolation of in situ data, but the interior analysis can be improved by leveraging surface data with regression or variational approaches. Here, we demonstrate how machine learning methods and satellite sea surface temperature, salinity, and height facilitate MLD estimation in a pilot study of two regions: the mid‐latitude southern Indian and the eastern equatorial Pacific Oceans. We construct multiple machine learning architectures to produce weekly 1/2° gridded MLD anomaly fields (relative to a monthly climatology) with uncertainty estimates. We test multiple traditional and probabilistic machine learning techniques to compare both accuracy and probabilistic calibration. We validate our methodology by applying it to ocean model simulations. We find that incorporating sea surface data through a machine learning model improves the performance of spatiotemporal MLD variability estimation compared to optimal interpolation of Argo observations alone. These preliminary results are a promising first step for the application of machine learning to MLD prediction.
Brown adipose tissue prevents glucose intolerance and cardiac remodeling in high-fat-fed mice after a mild myocardial infarction
Authors: Peres Valgas da Silva Carmem , Shettigar Vikram K. , Baer Lisa A. , Abay Eaman , Madaris Kendra L. , Mehling Mikayla R. , Hernandez-Saavedra Diego , Pinckard Kelsey M. , Seculov Nickolai P. , Ziolo Mark T. , Stanford Kristin I. 
Published: October 2021

Abstract
Background
Obesity increases the risk of developing impaired glucose tolerance (IGT) and type 2 diabetes (T2D) after myocardial infarction (MI). Brown adipose tissue (BAT) is important to combat obesity and T2D, and increasing BAT mass by transplantation improves glucose metabolism and cardiac function. The objective of this study was to determine if BAT had a protective effect on glucose tolerance and cardiac function in high-fat diet (HFD) fed mice subjected to a mild MI.

Methods
Male C57BL/6 mice were fed a HFD for eight weeks and then divided into Sham (Sham-operated) and +BAT (mice receiving 0.1 g BAT into their visceral cavity). Sixteen weeks post-transplantation, mice were further subdivided into ±MI (Sham; Sham-MI; +BAT; +BAT-MI) and maintained on a HFD. Cardiac (echocardiography) and metabolic function (glucose and insulin tolerance tests, body composition and exercise tolerance) were assessed throughout 22 weeks post-MI. Quantitative PCR (qPCR) was performed to determine the expression of genes related to metabolic function of perigonadal adipose tissue (pgWAT), subcutaneous white adipose tissue (scWAT), liver, heart, tibialis anterior skeletal muscle (TA); and BAT.

Results
+BAT prevented the increase in left ventricle mass (LVM) and exercise intolerance in response to MI. Similar to what is observed in humans, Sham-MI mice developed IGT post-MI, but this was negated in +BAT-MI mice. IGT was independent of changes in body composition. Genes involved in inflammation, insulin resistance, and metabolism were significantly altered in pgWAT, scWAT, and liver in Sham-MI mice compared to all other groups.

Conclusions
BAT transplantation prevents IGT, the increase in LVM, and exercise intolerance following MI. MI alters the expression of several metabolic-related genes in WAT and liver in Sham-MI mice, suggesting that these tissues may contribute to the impaired metabolic response. Increasing BAT may be an important intervention to prevent the development of IGT or T2D and cardiac remodeling in obese patients post-MI.
Obesity and Roux-en-Y gastric bypass drive changes in miR-31 and miR-215 expression in the human rectal mucosa
Authors: Breininger Stella Panagio , Sabater Laura , Malcomson Fiona Caroline , Afshar Sorena , Mann Jelena , Mathers John Cummings 
Published: October 2021

Abstract
Background/Objectives
Obesity increases colorectal cancer (CRC) risk. However, the effects of weight loss on CRC risk are unclear. Epigenetic mechanisms involving microRNAs that lead to dysregulated gene expression may mediate the effects of obesity and weight loss on CRC risk. We examined the effects of obesity and weight loss following Roux-en-Y gastric bypass (RYGB) on microRNA expression in the human rectal mucosa.

Subjects/Methods
We collected rectal mucosal biopsies from obese patients (n = 22) listed for RYGB and age- and sex-matched healthy non-obese Controls (n = 20), at baseline and six months post-surgery. We quantified microRNA expression in rectal mucosal biopsies using Next Generation Sequencing and bioinformatics analysis to investigate the likely functional consequences of these epigenetic changes.

Results
Compared with non-obese individuals, obese individuals showed differential expression of 112 microRNAs (p < 0.05). At six-months post-RYGB, when mean body mass had fallen by 27 kg, 60 microRNAs were differentially expressed, compared with baseline (p < 0.05). The expression of 36 microRNAs differed significantly between both i) obese and non-obese individuals and ii) obese individuals pre- and post-RYGB. Quantitative polymerase chain reaction (qPCR) demonstrated that expression of miR-31 and miR-215 was significantly (p < 0.05) higher, 143-fold and 15-fold respectively, in obese than in non-obese individuals. Weight loss, following RYGB, reduced expression of miR-31 and miR-215 to levels comparable with Controls. These differentially expressed microRNAs are implicated in pathways linked with inflammation, obesity and cancer.

Conclusion
Our findings show, for the first time, that obesity is associated with dysregulated microRNA expression in the human rectal mucosa. Further, surgically-induced weight loss may normalise microRNA expression in this tissue.
A human iPSC-astroglia neurodevelopmental model reveals divergent transcriptomic patterns in schizophrenia
Authors: Szabo Attila , Akkouh Ibrahim A. , Vandenberghe Matthieu , Osete Jordi Requena , Hughes Timothy , Heine Vivi , Smeland Olav B. , Glover Joel C. , Andreassen Ole A. , Djurovic Srdjan 
Published: October 2021

AbstractWhile neurodevelopmental abnormalities have been associated with schizophrenia (SCZ), the role of astroglia in disease pathophysiology remains poorly understood. In the present study, we used a human induced pluripotent stem cell (iPSC)-derived astrocyte model to investigate the temporal patterns of astroglia differentiation during developmental stages critical for SCZ using RNA sequencing. The model generated astrocyte-specific gene expression patterns during differentiation that corresponded well to astroglia-specific expression signatures of in vivo cortical fetal development. Using this model we identified SCZ-specific expression dynamics, and found that SCZ-associated differentially expressed genes were significantly enriched in the medial prefrontal cortex, striatum, and temporal lobe, targetingVWA5AandADAMTS19. In addition, SCZ astrocytes displayed alterations in calcium signaling, and significantly decreased glutamate uptake and metalloproteinase activity relative to controls. These results implicate novel transcriptional dynamics in astrocyte differentiation in SCZ together with functional changes that are potentially important biological components of SCZ pathology.
Survival of patients with chronic lymphocytic leukemia before and after the introduction of chemoimmunotherapy in Germany
Authors: Kajüter Hiltraud , Wellmann Ina , Khil Laura , Jöckel Karl-Heinz , Zhang Can , Fink Anna-Maria , Hallek Michael , Stang Andreas 
Published: October 2021

AbstractChronic lymphocytic leukemia (CLL) is the most common leukemia of adults in western countries. Therapy is indicated in symptomatic and advanced stages and has changed fundamentally since 2010 when rituximab, an anti-CD20 antibody, has been approved for treatment of CLL. Until then therapy had been based on chemotherapy drugs. This study investigates whether survival in CLL patients improved at the population level after the introduction of combined chemoimmunotherapy. Data from the cancer registry North-Rhine Westphalia was used to calculate relative survival (RS) by applying period analyses. Age-standardized 5-year RS increased from 79% in 1998–2002 (75% in 2003–2007) to 81% in the calendar period 2008–2012 and 88% in 2013–2016 for men and continuously from 71% in 1998–2002 to 92% in 2013–2016 for women. In CLL patients aged 15–69 years 5-year RS increased from 83% to 90% for men and from 82% to 94% for women after adding an anti-CD20-antibody to chemotherapy while in the older age group of 70–79-year-old CLL patients an increase by 20 percentage points was observed. These findings show marked improvements in the survival of CLL patients at the population level subsequently to the approval of anti-CD 20 antibodies like rituximab, ofatumumab or obinutuzumab for CLL treatment.
Reciprocal regulation between ER stress and autophagy in renal tubular fibrosis and apoptosis
Authors: Shu Shaoqun , Wang Hui , Zhu Jiefu , Liu Zhiwen , Yang Danyi , Wu Wenwen , Cai Juan , Chen Anqun , Tang Chengyuan , Dong Zheng 
Published: October 2021

AbstractBoth endoplasmic reticulum (ER) stress and autophagy have been implicated in chronic kidney injury and renal fibrosis. However, the relationship and regulatory mechanisms between ER stress and autophagy under this condition remain largely unknown. In this study, we first established a mouse model of ER stress-induced chronic kidney injury by 2 weekly injections of a low dose of tunicamycin (TM), a classical ER stress inducer. This model showed the induction of ER stress, autophagy, fibrosis and apoptosis in kidney tissues. In vitro, TM also induced ER stress, autophagy, fibrosis and apoptosis in HK-2 human kidney proximal tubular cells and BUMPT-306 mouse kidney proximal tubular cells. In these cells, autophagy inhibitor suppressed TM-induced fibrotic changes and apoptosis, suggesting an involvement of autophagy in ER stress-associated chronic kidney injury. PERK inhibitor ameliorated autophagy, fibrotic protein expression and apoptosis in TM-treated cells, indicating a role of the PERK/eIF2α pathway in autophagy activation during ER stress. Similar results were shown in TGF-β1-treated HK-2 cells. Interestingly, in both TM- or TGF-β1-treated kidney proximal tubular cells, inhibition of autophagy exaggerated ER stress, suggesting that autophagy induced by ER stress provides a negative feedback mechanism to reduce the stress. Together, these results unveil a reciprocal regulation between ER stress and autophagy in chronic kidney injury and fibrosis.
DCAF1-targeting microRNA-3175 activates Nrf2 signaling and inhibits dexamethasone-induced oxidative injury in human osteoblasts
Authors: Chen Jing , Liang Jin-qian , Zhen Yun-Fang , Chang Lei , Zhou Zhen-tao , Shen Xiong-jie 
Published: October 2021

AbstractActivation of nuclear-factor-E2-related factor 2 (Nrf2) signaling can protect human osteoblasts from dexamethasone-induced oxidative injury. DDB1 and CUL4 associated factor 1 (DCAF1) is a novel ubiquitin E3 ligase for Nrf2 protein degradation. We identified a novel DCAF1-targeting miRNA, miR-3175. RNA pull-down, Argonaute 2 RNA-immunoprecipitation, and RNA fluorescent in situ hybridization results confirmed a direct binding between miR-3175 and DCAF1 mRNA in primary human osteoblasts. DCAF1 3′-untranslated region luciferase activity and its expression were significantly decreased after miR-3175 overexpression but were augmented with miR-3175 inhibition in human osteoblasts and hFOB1.19 osteoblastic cells. miR-3175 overexpression activated Nrf2 signaling, causing Nrf2 protein stabilization, antioxidant response (ARE) activity increase, and transcription activation of Nrf2-dependent genes in human osteoblasts and hFOB1.19 cells. Furthermore, dexamethasone-induced oxidative injury and apoptosis were largely attenuated by miR-3175 overexpression in human osteoblasts and hFOB1.19 cells. Importantly, shRNA-induced silencing or CRISPR/Cas9-mediated Nrf2 knockout abolished miR-3175 overexpression-induced osteoblast cytoprotection against dexamethasone. Conversely, DFAC1 knockout, by the CRISPR/Cas9 method, activated the Nrf2 cascade and inhibited dexamethasone-induced cytotoxicity in hFOB1.19 cells. Importantly, miR-3175 expression was decreased in necrotic femoral head tissues of dexamethasone-taking patients, where DCAF1 mRNA was upregulated. Together, silencing DCAF1 by miR-3175 activated Nrf2 signaling to inhibit dexamethasone-induced oxidative injury and apoptosis in human osteoblasts.
Chromatin accessibility analysis identifies the transcription factor ETV5 as a suppressor of adipose tissue macrophage activation in obesity
Authors: Hu Ren-Dong , Zhang Wen , Li Liang , Zuo Zu-Qi , Ma Min , Ma Jin-Fen , Yin Ting-Ting , Gao Cai-Yue , Yang Shu-Han , Zhao Zhi-Bin , Li Zi-Jun , Qiao Gui-Bin , Lian Zhe-Xiong , Qu Kun 
Published: October 2021

AbstractActivation of adipose tissue macrophages (ATMs) contributes to chronic inflammation and insulin resistance in obesity. However, the transcriptional regulatory machinery involved in ATM activation during the development of obesity is not fully understood. Here, we profiled the chromatin accessibility of blood monocytes and ATMs from obese and lean mice using assay for transposase-accessible chromatin sequencing (ATAC-seq). We found that monocytes and ATMs from obese and lean mice exhibited distinct chromatin accessibility status. There are distinct regulatory elements that are specifically associated with monocyte or ATM activation in obesity. We also discovered several transcription factors that may regulate monocyte and ATM activation in obese mice, specifically a predicted transcription factor named ETS translocation variant 5 (ETV5). The expression of ETV5 was significantly decreased in ATMs from obese mice and its downregulation was mediated by palmitate stimulation. The decrease in ETV5 expression resulted in macrophage activation. Our results also indicate that ETV5 suppresses endoplasmic reticulum (ER) stress and Il6 expression in macrophages. Our work delineates the changes in chromatin accessibility in monocytes and ATMs during obesity, and identifies ETV5 as a critical transcription factor suppressing ATM activation, suggesting its potential use as a therapeutic target in obesity-related chronic inflammation.
EIF4A3-induced circular RNA PRKAR1B promotes osteosarcoma progression by miR-361-3p-mediated induction of FZD4 expression
Authors: Feng Zhen-hua , Zheng Lin , Yao Teng , Tao Si-yue , Wei Xiao-an , Zheng Ze-yu , Zheng Bing-jie , Zhang Xu-yang , Huang Bao , Liu Jun-hui , Chen Yi-lei , Shan Zhi , Yuan Pu-tao , Wang Cheng-gui , Chen Jian , Shen Shu-ying , Zhao Feng-dong 
Published: October 2021

AbstractEmerging evidence indicates that circRNAs are broadly expressed in osteosarcoma (OS) cells and play a crucial role in OS progression. Recently, cancer-specific circRNA circPRKAR1B has been identified by high-throughput sequencing and is recorded in publicly available databases. Nevertheless, the detailed functions and underlying mechanisms of circPRKAR1B in OS remains poorly understood. By functional experiments, we found that circPRKAR1B enhanced OS cell proliferation, migration, and promotes OS epithelial–mesenchymal transition (EMT). Mechanistic investigations suggested that circPRKAR1B promotes OS progression through sponging miR-361-3p to modulate the expression of FZD4. Subsequently, we identified that EIF4A3 promoted cirPRKAR1B formation through binding to the downstream target of circPRKAR1B on PRKAR1B mRNA. Further rescue study revealed that overexpression of the Wnt signalling could impair the onco-suppressor activities of the silencing of circPRKAR1B. Interestingly, further experiments indicated that circPRKAR1B is involved in the sensitivity of chemoresistance in OS. On the whole, our results demonstrated that circPRKAR1B exerted oncogenic roles in OS and suggested the circPRKAR1B/miR-361-3p/FZD4 axis plays an important role in OS progression and might be a potential therapeutic target.