bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial assessment of milk collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh and determination of antimicrobial resistance patterns of the isolated bacteria
Authors: Hossain Mohammad Farhad , Rahman Md Tanvir , Kabir SM Lutful 
Published: September 2021

The study was conducted to determine the total viable count (TVC) and total coliform count (TCC) of unpasteurized, pasteurized and UHT milk samples to assess their microbiological quality. A total of 60 milk samples were collected from different markets of Mymensingh, Gazipur and Sherpur districts. The TVC of milk was performed to determine the bacterial load in supplied milk samples and TCC to determine the coliform bacterial load in collected milk samples. Milk samples were cultured onto various selective media for the isolation of bacteria. The isolated bacteria were identified by cultural properties on different selective media, biochemical tests and finally by PCR. Out of 60 samples 20 unpasteurized milk samples from different markets were found positive for Staphylococcus aureus and 19 unpasteurized milk samples were found positive for Escherichia coli. All the S. aureus were found positive for S. aureus specific 16S rRNA gene by PCR. Out of 19 isolates of E. coli, 15 were found positive for E. coli 16S rRNA gene by PCR. Results of antimicrobial susceptibility test showed that most of the isolates of S. aureus and E .coli were susceptible to azithromycin, streptomycin, gentamicin, norfloxacin, tetracycline and ciprofloxacin but resistant to amoxicillin and erythromycin. The findings of this study revealed the presence of multidrug resistant S. aureus and E. coli in unpasteurized milk samples that posseses a serious threat to public health.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 7-16
Isolation, molecular identification and characterization of Aeromonas hydrophila from infected air-breathing catfish Magur (Clarias batrachus) cultured in Mymensingh, Bangladesh
Authors: Monir Md Shirajum , Bagum Nazneen , Kabir SM Lutful , Borty Shuvho Chakra , Ud Doulah Mohammad Ashaf 
Published: September 2021

The study was carried out from November, 2014 to February, 2015 with the objective of isolating Aeromonas hydrophila an important fish pathogen from infected air-breathing catfish Magur (Clarias batrachus) in Mymensingh district. Quantitative study of the isolated bacteria from infected C. batrachus was found variation of number in different organs. Total bacterial load was found to be 1.16 x 105 to 3.15 x 106 cfu/g in lesions, 2.14 x 108 to 4.17 x 109 cfu/g in liver, 1.90 x 107 to 5.12 x 108 cfu/g in spleen and 2.32 x 106 to 5.24 x 108 cfu/g in kidney of infected C. batrachus in Mymensingh district. The isolates were found to produce acid from arabinose, whereas acid and gas from different sugar media such as maltose, sucrose, and dextrose. Morover, they were capable to ferment glucose but resistant to vibriostatic agent 0129 test. Further identification of A. hydrophila was accomplished using PCR. The PCR products of desired 760 bp were obtained for A. hydrophila. The isolated A. hydrophila were 96% sensitive to Enrofloxacin followed by 88% to Ciprofloxacin and 76% to Levofloxacin. On the other way, 100% were resistant to the Ampicillin followed by 96% to Penicillin and 92% to Novobiocin. So far, this is the first molecular identification of A. hydrophila from farmed C. batrachus in Bangladesh. The present study will provide future research scopes on identification of pathogenicity island in chromosome and serotyping of all A. hydrophila isolates.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 17-24
Comparative study on nutritional status, food intake pattern and lifestyle between dialyzed and non-dialyzed kidney patients in Dhaka, Khulna and Rajshahi divisions of Bangladesh
Authors: Islam Md Tanvir , Mukta Nurun Nahar 
Published: September 2021

Chronic kidney disease (CKD) is a worldwide public health problem. As per nephrologists' estimation, the CKD population grows at approximately 5% annually and in 2022, over 29 million people will be afflicted with CKD in Bangladesh. The objective of this study was to compare nutritional status, food intake pattern, and lifestyle between dialyzed and non-dialyzed kidney patients in Bangladesh. It was a cross-sectional comparative study of patients aged 18 to 85 year old to compare nutritional status, food intake pattern, and lifestyle between dialyzed and non-dialyzed kidney patients. Sample was conducted in three divisions Dhaka, Khulna and Rajshahi in Bangladesh. Data on food intake pattern, height, weight, GFR, other diseases along with kidney disease, physical activity, smoking and socioeconomic status etc. were collected from fifteen clinical centers and sometimes from household via in-person interviews. A total of 244 patients aged were selected among them 152 people were dialyzed patients and 92 people were Non-dialyzed patients. We found a relationship among dietary food habit, lifestyle factors and health outcomes such as chronic kidney disease progression, heart disease and death. Patients had a mean monthly family income of 23683.46, a mean monthly expenditure on food of 3815.2 and a mean monthly expenditure on treatment of 13317.69. According to our findings, highest dialysis patients were sedentary than non-dialysis patients were active at their active life (20-40 Years). From this study it was investigated that, 41.0% patients have not changed their addiction habit after diagnosis, where 20.1% dialysis patients and 20.9% non-dialysis patients have not changed their addiction habit totally. Another significant finding of this study was that dialysis patients had poor consumption from 16 groups. It can be highlighted that, the prevalence of the diseases were higher in urban people than rural people. This study suggests that additional research is needed to investigate the optimal dietary recommendations and body mass index levels to prevent disease progression and poor outcomes among individuals with CKD. The prevalence of HD-CKD and NDD-CKD can be said to be SES dependent. Chronic kidney disease and its complications, which involve most organ systems, can be prevented, but awareness and use of accurate methods are needed to enable timely diagnosis.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 25-34
Assessment of nutritional status and other associated factors of lactating mother at Gangni Pauroshava in Meherpur district, Bangladesh
Authors: Islam Md Tanvir , Zahid Md Ashrafuzzaman , Hossain Md Ismail , Parvin Rashida 
Published: September 2021

The present study was to provide information about the nutritional status of lactating women as well as proffer solutions that are aimed at improving maternal nutrition in the study area. The study area was Gangni union of Meherpur district. 105 exclusively breastfeeding women aged 16-35 years old were initially enrolled in the study. Of these study lactating women completed 24h dietary recall food intake. The findings was about 16.2% lactating mothers were under 19 years, 53.3% respondents were between the ages of 20-25 years, 23.8% respondents were between the ages of 26-30 years and 37.1% lactating mothers had completed primary education. About 95.2% respondents were housewife and 4.8% respondents were service holders. The finding was 56.2% respondents have one child and 38.1% respondents have 3 family members, 43.8% respondent has 4 family members. About 7.6% respondents are underweight, 55.2% respondents are normal, 33.3% respondents are overweight and 3.8% respondents are obese. Total 7.6% respondents are underweight, 55.2% respondents are normal, 33.3% respondents are overweight and 3.8% respondents are obese where 5.7% respondents under 19 year aged and 1.9% respondents over 30 year aged are underweight. About 40% respondents got surgerian delivery where 60% respondents got normal delivery. It was found that 100% respondents maintain hygiene such as washing hands before eating foods, washing hands after using bathroom, brushing teeth regularly, avoiding nasty foods etc.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 35-44
Evaluation of pathogenicity of motile Aeromonas species in air-breathing catfish Magur (Clarias batrachus)
Authors: Monir Md Shirajum , Bagum Nazneen , Kabir SM Lutful , Borty Shuvho Chakra , Ud Doulah Mohammad Ashaf 
Published: September 2021

The present study was carried out to evaluate the comparative capability of producing infections and causing mortality of the experimental Magur (Clarias batrachus) with motile Aeromonas species. A total of 200 apparently healthy C. batrachus were acclimatized to the laboratory conditions for experimental study. Nine different groups (each group consisting of 20 fish) of healthy C. batrachus was injected with nine motile Aeromonas isolates (A. hydrophila-3, A. sobria-3 and A. caviae-3). Experimental C. batrachus were infected with motile A. hydrophila, A. sobria and A. caviae to groups 1-3, 4-6 and 7-9, respectively while group 10 was injected with sterile physiological saline (0.85% NaCl) and served as the control. The selected motile bacterial species via intramuscularly were injected at the rate of 4.5 × 105 cfu/fish for pathogenicity study on C. batrachus and monitored up to two weeks. The highest clinical infections were noticed 90% in group-3 whereas only 35% in group-8 within the experimental period. After two weeks of the experiment, the cumulative mortality rate was also found highest (60%) in group-3 but lowest (15%) in group-9 while no infection or mortality showed in group-10 (control group). The development of infection and mortality to the injected C. batrachus was associated more severely by Aeromonas hydrophila than A. sobria and A. caviae used in this study. However, the isolates motile Aeromonas species could serve as the primary cause of skin lesions as well as mortality in cultured C. batrachus.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 45-50
Effect of dietary probiotics on the growth performance, meat quality improvement of broiler chicken for safe meat production
Authors: Sarker Md Sazedul Karim , Rana Md Masud , Sultana Shabiha , Sarker Nathu Ram , Nahar Talukder Nurun 
Published: September 2021

A feeding trial was conducted to investigate the effect of Probiotic (Biofast) on the growth performance and meat quality of broiler chicken. Ninety six 1-d-old mix sexed Cobb 500 broiler chicks were obtained from a local commercial hatchery. Chicks were randomly allocated in 2 experimental treatments for 5 wk. The experimental treatments received a 1) control, 2) control with probiotic Biofast 0.055% (bacillus subtilis-100%) in the diet. Both treatments had 48 broilers arranged in 4 replicates of 12 broilers each. Eight birds from 2 different groups (one/replication) were sacrificed on termination of the feeding trial of breast, thigh to investigate the meat quality. Feed intake in treatment T1 was significantly (P > 0.05) greater than control in 0 to 3 weeks of age. Though in starter phase treatments failed to induce any marked effects on body weight, weight gain and FCR but numerically increased in T1 than control. In finisher period (4-5 wks), there were no significant differences on body weight, weight gain, feed intake and FCR between the treatments. The organ weight like liver, heart, kidney, spleen, gizzard, abdominal fat and intestine weight also did not show any significant differences between the dietary treatments of control and Biofast. In addition, though the dressing % was not significantly different but numerically higher percentage was found by using Biofast. Similarly, no significant differences (p>0.05) were found in pH, cooking loss, meat color and TBA values between the treatments. In conclusion, supplementation of Biofast in diet has no significant effect on the growth performance of broiler chicks though it has got some positive effects on other parameters that indirectly revealed to enhance meat quality of broiler chicken as well as food safety issues.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 51-57
Microbiological quality assessment and acceptance of dairy products in Dhaka city
Authors: Hossain Md Nur , Humayun Sanjida , Haque Md Zahurul , Ahmed Monzur Morshed 
Published: September 2021

This investigation was carried out to evaluate the microbiological quality of the processed dairy products including borhani, matha/labang, sweet & sour yoghurt. Dairy products are consumed as desert and popular enough among the people. 50 of the different dairy samples were collected from street vendor and also from some branded shop in Dhaka city. The microbiological quality of the samples were analyzed and monitored according to criteria in European Commission Recommendations 2004/24/EC and 2005/175/EC, BSTI and USPHS. All the dairy products had high microbial load ranged 5.90×105cfu/ml to 8.97×109 cfu/ml. Coliforms were found up to 109 dilutions in milk based drink products Borhani and Labang considered a serious threat to the public health. The mold contamination was much lower in Borhani and Labang compared to yoghurt 7.86×108cfu/ml. Approximately 17% dairy products were contaminated by Salmonella spp. Majority of the dairy products (25.537%,) such as Yogurt, Borhani and Labang were contaminated by Staphylococcus spp. The presence of Listeria monocytogenes found in Yogurt, 7 out of 21 (21.515%) samples were contaminated. A minor number of milk based products were corrupted by Shigella spp. These results emphasize applying and maintaining good hygiene practices throughout the processing chain to prevent contamination and bacterial growth. It was concluded that the presence of some pathogens in milk based products with toxigenicity of some strains pose a health hazard to consumers. Thus, good hygienic practices, good manufacturing practices, HACCP with all other hygienic practices should be applied during processing and distribution for public health safety.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 58-64
Effects of gamma irradiation on shelf life and quality of Black Bengal goat meat
Authors: Ruman Khan Saddam Hossain , Salahuddin Md , Habib Md Rezwanul , Billah Md Mostain , Haris Miah Md Abu , Akhter Shajeda , Hashem Md Abul 
Published: September 2021

This study was undertaken to assess the effects of gamma irradiation (0, 1.5, 2, 4 kGy) on shelf life and meat quality of Black Bengal goat. About 3 kg of fresh Black Bengal goat meat samples were taken and divided into four groups like T1 (non-irradiated, control), T2 (irradiated, 1.5 KGy), T3 (irradiated, 2.0 KGy) and T4 (irradiated, 4.0 KGy). Irradiated and non-irradiated meat samples were stored at refrigeration temperature to elucidate the storage effect. One way ANOVA was performed to investigate the effects of gamma irradiation on different groups. Significant differences were found in color and overall acceptability compared to non-irradiated group and only overall acceptability significantly decreased with increasing storage. No significant differences were observed in flavor, tenderness and juiciness between non-irradiated and irradiated groups. Irradiation influenced color and overall acceptability of chevon. Dry matter content was found higher in 4 KGy irradiated group and increased gradually in day intervals. Cooking loss, free fatty acid, peroxide value and thiobarbituric acid-reactive substances indicated 1.5 KGy irradiation rated best. Microbial findings revealed that 2 KGy irradiated group is better due to safe level of microbial loads which increase shelf life of Black Bengal goat meat. Finally, it may be concluded that 1.5 and 2 KGy doses gamma irradiation in Bengal goat meat enhances sensory attributes, physico-chemical and microbial levels found satisfactory.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 65-73
Evaluation of commonly used fertilizers, fish and poultry feeds as potential sources of heavy metals contamination in food
Authors: Sarker Md Sanaullah , Quadir Quazi Forhad , Hossen Md Zakir , Nazneen Touhida , Rahman Atiqur 
Published: September 2021

An experiment was conducted at the Department of Agricultural Chemistry, BAU to assess the heavy metal status in different feed and fertilizer samples collected from Sadar and Trisal upazilas of Mymensingh district. The feed samples included poultry and fish feeds, whereas, fertilizer samples included DAP, TSP, MOP, gypsum and zinc sulphate (ZnSO4). AAS analysis of Cd, Cr and Pb showed variable levels of contamination in the fertilizer and feed samples. In case of feed samples, Cd, and Pb contents in fish feed collected from Sadar upazila was 0.159 and 3.581 mg kg-1, respectively and that of Trisal upazila was 0.441 and 153.824 mg kg-1, respectively. The content of Cd was 0.008 mg kg-1 in poultry feed but in fish feed it was 0.441 mg kg-1. The Cr content in both poultry and fish feeds collected from Sadar and Trisal upazilas were below the detection level. In general, heavy metals contaminations were higher in different fish feed samples compared to that of poultry feed samples, especially in samples from Trisal upazila.The Cd contents in DAP, TSP, MoP, gypsum and ZnSO4 collected from Sadar upazila were 1.560, 14.749, 0.137, 0.906 and 10.312 mg kg-1, respectively, and that of Trisal upazila were 1.780, 15.662, 3.988, 1.180 and 3.165 mg kg-1, respectively. The Cr contents in DAP, TSP, MoP, gypsum and ZnSO4 collected from Sadar upazila were 60.392, 206.667, 15.882, 24.314 and 11.373 mg kg-1, respectively. Whereas, mean Cr concentrations DAP, TSP, MoP and gypsum from Trisal upazila were 54.118, 168.431, 16.078 and 26.863 mgkg-1, respectively. However, in ZnSO4Cr was below the detection limit. Again, Pb contents in DAP, TSP, MoP, Gypsum and ZnSO4 collected from Sadar upazila were 12.633, 45.729, 33.586, 13.319 and 30.014 mg kg-1, respectively, and that of Trisal upazila were 4.062, 46.919, 101.205, 21.681 and 6.676 mg kg-1, respectively. In general, heavy metal contamination levels in different fertilizer samples collected from Mymensingh Sadar were higher compared to that of Trisal upazila. The results showed that despite the close proximity of the market locations, heavy metal contents may vary in fertilizer and feed samples indicating various supply chains of these essential inputs are in place and calls for greater degree of quality monitoring from the government point of view.
Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 74-81
Detection of heavy metals in poultry feed, meat and eggs
Authors: Rashid MA , Sarker MSK , Khatun H , Sarker NR , Ali Md Yousuf , Islam MN 
Published: September 2021

The poultry feed, meat and egg samples were collected from the major poultry raising areas of the country to know the presence of heavy metals especially arsenic (As), lead (Pb) and chromium (Cr). A total of 360 elemental samples for As, Pb and Cr were analyzed in the laboratory. The heavy metal contents (As, Pb & Cr) of all the tested samples were found positive. But, the levels were below the Maximum Permitted Concentration (MPC) in most cases. The layer and broiler ready feed samples found safe from those elements; On the other hand, the “Cr” and “Pb” content in 14% and 11% loose feed samples were 7-70 and 3 times respectively higher than that of MPC. Notes worthy, the egg samples were also found safe from those elements. Of the tested samples 14% broiler meat and 50% spent hen samples found slightly higher level of “As” and 4-6 times “Cr” respectively than the MPC. In conclusion, the poultry farmers are suggested to buy poultry feeds from the reputed feed company and discourage to use loose feeds. Moreover, feed marketing channel particularly loose feed marketing should be monitored properly by the regulatory authority.
Asian Australas. J. Food Saf. Secur. 2018, 2(1), 1-5
Isolation and characterization of antibiotic resistant bacteria from pre and post-microwave oven treated burger sold in Mymensingh
Authors: Haider Mohammad Arif , Ievy Samina , Sobur Md Abdus , Mahmud Md Muket , Hossain Muhammad Tofazzal , Rahman AM Md Taufiqur , Nazir KHM Nazmul Hussain , Rahman Md Tanvir 
Published: September 2021

Burger is one of the common popular fast food. The present study was carried out to isolate and characterize bacteria from burger sold in Mymensingh that could be potential threat to human health. A total of 20 burger samples (10 belonged to pre-microwave oven treated group and 10 belonged to post-microwave treated group) were collected randomly from different bakeries and fast food shops that sell burgers in Mymensingh. Isolation and identification of bacteria were carried out based on morphology, staining, cultural and biochemical tests. All the samples were found contaminated with bacteria. Total viable count (TVC), total staphylococcal count (TSC) and total coliform count (TCC) of pre-microwave oven burger samples were 7.22±0.20 log CFU/g, 6.93±0.30 log CFU/g and 3.28±0.64 log CFU/g, respectively. Similarly in post-microwave oven treated burger the TVC, TSC and TCC were 3.09±0.42 log CFU/g, 2.84±0.56 log CFU/g and 1.60±0.42 log CFU/g, respectively. Microwave heat treatment for 60 seconds markedly reduced the TVC, TSC, TCC in the burger compared to pre microwave treated group. Many of the both the pre and post-microwave oven treated burgers were found positive for Staphylococcus spp. and E. coli. The isolates of Staphylococcus were found coagulase negative and resistant to Tetracycline while sensitive to Azithromycin, Gentamycin, Kanamycin and Streptomycin. On the other hand, the isolates of E. coli were found resistant to Azithromycin and Streptomycin while sensitive to Gentamycin, Kanamycin and Tetracycline. Data of this study suggested that burgers sold in the fast-food restaurant were contaminated with antibiotic resistant Staphylococcus and E. coli that might be considered as human health hazard.
Asian Australas. J. Food Saf. Secur. 2018, 2(1), 6-11
Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species
Authors: Hasan Mahedi , Kabir SM Lutful , Rahman Md Tanvir , Sarker Yousuf Ali 
Published: September 2021

The research project was conducted to assess the bacteriological quality of buffalo meat samples collected from three upazilas namely Haluaghat, Sreepur and Madhupur of Bangladesh under the districts of Mymensingh, Gazipur and Tangail respectively with particular emphasis on the molecular detection and antimicrobial resistance of the isolate Salmonella species. Total viable count (TVC), total staphylococcal count (TStaC) and total salmonella count (TSC) of meat samples were determined and the mean values of TVC, TStaC and TSC for the Haluaghat, Sreepur and Madhupur were log 8.30, log 7.94, log 8.15; log6.21, log 6.40, log 5.43 and log 4.76, log 4.82, log 4.56 CFU/gm respectively which exceeded the ICMSF recommendations values. The variation of TVC and TSC in meats of different buffalo markets was significant at 5% level where the variation of TStaC was significant at 1% level. Nevertheless no significant variation was demonstrated between the interactions of the three upazilas. Among the samples, 46.67% (n=14) were found to be associated with Salmonella spp. The Salmonella spp. were identified by observing black centered colonies on XLD agar, positive to MR test and negative to VP and Indole test. All isolates of Salmonella spp. were positive to 16s rRNA gene based PCR (574bp). All isolates of Salmonella species were susceptible to ciprofloxacin, streptomycin and gentamicin. All isolates of Salmonella spp. (n=14; 100%) were resistant to amoxicillin and few isolates also resistant to erythromycin, tetracycline, azithromycin and cephradine. The findings of this study revealed the presence of multidrug resistant Salmonella spp. in buffalo meat of Mymensingh, Gazipur and Tangail districts of Bangladesh that posseses a serious threat to public health.
Asian Australas. J. Food Saf. Secur. 2018, 2(1), 12-20