bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Diagnostic Criteria for MELAS Syndrome; a Case Study Involving an Elderly Patient With MT-TWm.5541C&gt;T Mutation
Authors: Wu Gang , Shen Yuguang , Zhu Feng , Tao Weiwei , Zhou Yuanlin , Ke Shaofa , Jiang Huihua 
Published: October 2021

Abstract
Background: Mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome is a matrilineal hereditary multi-system disease due to mutations of mitochondrial DNA. Though the initial diagnostic criteria prevail till nowadays, which take a range of clinical phenotypes into consideration including clinical onset after the age of 40, a confirmative diagnostic standard for MELAS is still needed.Case presentation: A 71-year-old female patient presented with recurrent stroke to our hospital. Magnetic resonance imaging showed a cerebral gyrus-like area with hyperintensity in the parietal-occipital lobe on DWI and this area expanded with disease progression. The Lac/Lip peaks were significantly inverted in the MRS imaging. Nucleic acid sequencing showed a MT-TWm.5541C>T mutation, and the mutation rate of the blood sample was 12.86%. The patient had a 6-year history of type 2 diabetes. The patient experienced recurrent stroke and was given coenzyme Q10 capsules and idebenone tablets to improve metabolism, vitamin B to nourish nerves, edaravone to scavenge oxygen free radicals, and oxcarbazepine to manage seizures. At 1-month follow-up, her condition was stable.Conclusion: Patients with MELAS syndrome have complex clinical manifestations. Our data demonstrated that, for patients with atypical cerebral infarction and suspected MELAS syndrome, gene sequencing and muscle biopsy should be completed in time, which may serve as a reference for the diagnostic criteria of MELAS syndrome.
Correlation and Predictive Ability Between Sensory Characteristics and Social Interaction of Children in Autism Spectrum Disorder
Authors: Zhai Jinhe , Fan Lili , Zhou Yong , Li Yutong , Li Xiaoxue , Xia Wei , Wang Jia , Wu Lijie 
Published: October 2021

Abstract
Background: As a kind of neurodevelopmental disorder, the deficiency of social interaction and communication ability is the core symptom of autism spectrum disorder (ASD). However, ASD usually has sensory abnormalities, which may be an important influencing factor of social function. Here, the aim of the current study is to explore the abnormal sensory characteristics of ASD children and the correlation between social behavior. And further clarify the predictive effect of sensory expression on ASD social ability.Methods: A case-control study was conducted, with children aged 3 to 10 years including ASD and typical development (TD) as subjects. We used Short Sensory Profile (SSP) questionnaire to evaluate the sensory characteristics in ASD group and TD group. And Social Responsiveness Scale (SRS) was employed to assess the social function of ASD. The differences of SSP scores between two groups of children were compared, and the correlation between SSP in ASD group and SRS was further analyzed. Furthermore, by constructing random forest classification model and support vector machine classification model, the predictive ability of each perception on social level is discussed.Results: Significant differences were found between ASD children and TD children in the performance of each sensory field. The abnormal sensory rate of children in ASD group is as high as 91.4%, and 74% of them are accompanied by sensory disorders in multiple dimensions.It is worth noting that there is a significant negative correlation between the scores of ASD group children in all dimensions of SSP and the scores of SRS scale. Evaluation and comparison results of full-feature and 7-feature models show that the random forest model and SVM model with all-feature factors have higher sensitivity, while the random forest model with 7-feature factors has the highest specificity. Moreover, the maximum of area value under (AUC) of receiver operating characteristic (ROC) curve between the four models up to 0.859, suggest that the good prediction effect of the models.Conclusion: Our results suggest autism children have obvious abnormalities in many sensory fields, and there is a significant correlation between this atypical sensory performance and social function. The social level of this group can be well predicted by their sensory characteristics.
Improvement of physical activity was significantly reduced serum hepatocyte growth factor levels in a general population: 10-year prospective study
Authors: Sakaue Akiko , Adachi Hisashi , Enomoto Mika , Fukami Ako , Nohara Yume , Morikawa Nagisa , Yamamoto Maki , Satoh Hiromi , Fukumoto Yoshihiro 
Published: October 2021

Abstract
Background: We have previously reported that hepatocyte growth factor (HGF) levels were significantly associated with insulin resistance or components of the metabolic syndrome. However, it has been unknown how physical activity (PA) affects HGF levels after a long-term follow-up.Aims: Our aim was to clarify the association between PA changes and HGF levels /cerebro-cardiovascular disease (CVD) development during 10 years follow-up period in a community-dwelling Japanese general population.Methods: Of 1,320 subjects who received a health check-up examination in Tanushimaru town in 1999, 903 subjects (341 males and 562 females) were enrolled, who received the examination both in 1999 and 2009. We evaluated their PA levels by Baecke questionnaire in 1999 and evaluated their PA levels by a simple questionnaire in 2009. We measured the serum HGF levels by ELISA method in 1999 and 2009. We divided into four physical activity groups such as stable low PA, increased PA, decreased PA, and stable high PA. Using these questionnaires, we compared their PA and HGF levels in 10 years.Results: A significant inverse association was found between PA changes and serum HGF levels in 10 years after adjustment for age and sex. The serum HGF levels of increased PA group were significantly lower than stable low PA (p=0.038), and the former group showed the reduced CVD development compared to the latter group after adjustment for age and sex (p=0.012).Conclusions: Our data demonstrated that improvement of PA levels was associated with reduced HGF levels and CVD development.
Role of Big data in HealthCare and Internet of Things: A Detailed Bibliometric Survey
Authors: Addepa Phani sridhar , Tenneti Krishna Mohana 
Published: October 2021

Abstract
This paper makes the Bibliographic study of the Big Data involvement in Healthcare and Internet of things. Research contribution towards this area has started from the year 2013 as per the fundamental keywords chosen in this survey. Thus makes the thrust discipline and emerging technology of research, also a trendy domain based on its recognized inception. Upon consideration of the selected keywords there was no work up to 2012 and all together 425 papers are obtained from 2013 onwards through Scopus website. Among these major contribution was in Computer Science Subject area with 308 in number and maximum articles were published in English Language with 421 and highest number of papers contributed were 128 by India. This study uses versatile Bibliometric analysis databases and methods resulting in Statistical and Network Analysis in terms of Authors, Organizations, Citations, Co- Citations etc.
Synthesis and Physicochemical Properties of Polyamideimide Membranes Containing ZIF-8 and CMS Particles for Potential Gas Separation Applications
Authors: Balaguru Sasikumar , Sabilon Yohannan Subin , Gangasalam Arthanareeswaran 
Published: October 2021

Abstract
The present study involves the fabrication and characterization of Polyamideimide (PAI) membrane holding ZIF-8 and CMS particles for potential gas separation applications. Zeolitic imidazolate frameworks-8 (ZIF-8) nanocrystals were prepared by a precipitation reaction with Zinc metal cluster and 2-methylimidazole, whereas the carbon molecular sieves (CMS) were synthesized by pyrolysis of polyamideimide (PAI) polymer. ZIF-8 and CMS particles were characterized comprehensively for the functional group, crystallinity, and morphological analyses. The successful formation of ZIF-8 nanocrystals was evident from the rhombic octahedron shape, and EDX confirms the presence of Zn metal cluster and methylimidazole linker. The ZIF-8 and CMS nanoparticles incorporated PAI membranes were prepared using phase inversion technique with varying loading wt.% of 1, 2, and 3%. PAI/ZIF-8 and PAI/CMS membranes cross-sectional morphology confirmed that synthesized nanoparticles were well embedded through the PAI membrane. The PAI/ZIF-8 (3 %) membrane, thin dense skin top layer, and well-defined honeycomb porous substructure were observed. Furthermore, the ZIF-8 and CMS particles incorporation have a beneficial impact on the mechanical properties of PAI at the low loading of nanoparticles. Thus, the inclusion of ZIF-8 and CMS particles in the PAI matrix positively altered the physicochemical properties of the resulting hybrid membranes, which could help them achieve remarkable gas permeance and selectivity.
Discovery of Oxazolidinone-Based Heterocycles as Subtype Selective Sigma-2 Ligands
Authors: Blass Benjamin E , Bhandare Richie Rashmin , Canney Daniel J. 
Published: October 2021

Abstract
Alzheimer’s disease is a major, unmet medical need that impacts 6 million people in the US alone. Therapeutic options are limited, and the root cause of this condition remains unclear. The Amyloid Hypothesis has been proposed as a means of explaining the formation of amyloid plaques in the brain of patient. The sigma-2 receptor was recently identified as a potential therapeutic target capable of arresting the formation of amyloid plaques. Herein, we report the identification of a series of novel, functionalized oxazolidin-2-ones sigma-2 ligands.
Recovery from Severe Neurological Sequelae due to Anti-N-methyl D-aspartate Receptor Encephalitis after Three Infusions of Allogeneic Umbilical Cord-derived Mesenchymal Stem Cells: A First Case Report
Authors: Nguyen Liem Thanh , Hoang Van T. , Thu Huong Le , Nguyen Phuong Anh Thi , Hoang Duc M. , Ngo Doan Van , Vu Hung Cao , Bich Van Nguyen Thi , Heke Michael 
Published: October 2021

Abstract
PurposeAnti-N-methyl D-aspartate (NMDA) receptor encephalitis is caused by altered patients’ immune reactions. This study reports the first patient with severe neurologic sequelae after NMDA receptor encephalitis treated with allogeneic umbilical cord-derived mesenchymal stem/stromal cells (UC-MSCs) via intrathecal route.MethodsUC-MSCs were obtained from a healthy donor and expanded under a xeno- and serum-free condition. The patient received three intrathecal UC-MSC infusions at the dose of 106 cells/Kg. The outcome was accessed using the German Coma Recovery Scale (CRS), the Gross Motor Function Classification System (GMFCS), the Gross Motor Function Measure–88 (GMFM-88), Manual Ability Classification System (MACS), Modified Ashworth Scale, and the Denver II test.ResultsA 5-year-old girl suffered from a permanent vegetative state with diffuse cerebral atrophy due to NMDA receptor encephalitis despite intensive treatment of immunosuppressive medicaments and IVIG. After three UC-MSC infusions, her cognition and motoric functions improved progressively. In the last visits, she could walk, practice writing, and count numbers. Urinary and bowel functions were completely controlled. Cerebral atrophy reduced on brain MRI.ConclusionsThe outcomes of this patient suggest a potential cell therapy for autoimmune encephalitis and its neurological consequences.
Factors Affecting Adherence to Early Mobilization in an Intensive Care Unit: An Analysis of Data Collected Prospectively Over a Three-Year Period by Clinical Information System
Authors: Perelló Paula , Gómez Josep , Mariné Judith , Cabas Maria Teresa , Arasa Alba , Ramos Zaida , Moya David , Reynals Irene , Bodí María , Magret Mònica 
Published: October 2021

Abstract
Background: Early mobilization benefits critically ill patients. Scant information is available about adherence to early mobilization protocols in intensive care units (ICU). With the aim of optimizing the application of our early mobilization protocol in mechanically ventilated patients in routine daily practice, this study analyzed adherence to the protocol, impediments to adherence, and adverse eventsin our ICU. Methods: This observational study analyzed data collected prospectively at a 24-bed polyvalent ICU over a three-year period (2017–2019). Data from adult patients on mechanical ventilation > 48 hours who met the inclusion criteria for the early mobilization protocol were included. We analyzed demographics, adherence to the protocol, total number of mobilizations, impediments to early mobilization, artificial airway/ventilatory support at each mobilization level, and adverse events during mobilization. All data was automatically obtained from the clinical information system by extract, transform, and load processes using Python 3.0. The unit of analysis was ICU stay-day. Results: We analyzed 3269 stay-days from 388 patients with median age of 63 (51–72) years, median APACHE II 23 (18–29) and median ICU stay of 10.1 (6.2–16.5) days. Adherence to the protocol was 56.6% (1850 stay-days), but patients were mobilized in only 32.2% (1472) of all stay-days. The most common reasons for not mobilizing patients were failure to meeting the criteria for clinical stability in 241 (42%) stay-days and unavailability of physiotherapists in 190 (33%) stay-days. Adverse events occurred in only 6 (0.4%) stay-days mobilizations. Conclusions: Although adherence to the protocol was high, patients were mobilized in only one-third of all stay-days. This study points to ways we can improve early mobilization in our ICU, including assessing the suitability of the criteria for clinical stability and increasing the presence of physiotherapists.
Nanoscopic resolution within a single imaging frame
Authors: García Esley , Cámara Raúl , Linares Alejandro , Martínez Damián , Abonza Víctor , Brito-Alarcon Eduardo , Calcines-Cruz Carlos , Galindo Gustavo , Torres David , Jabloñski Martina , Torres-Martínez Héctor H. , Martínez José , Hernández Haydee , Ocelotl Joseph , Garcés Yasel , Barchi Marco , Dubrovsky Joseph G. , Darszon Alberto , Buffone Mariano , Rodríguez Roberto , Rendón-Mancha Juan , Wood Christopher , Hernández Armando , Krapf Diego , Crevenna Álvaro , Guerrero Adán 
Published: October 2021

Abstract
Mean-Shift Super Resolution (MSSR) is a principle based on the Mean Shift theory that improves the spatial resolution in fluorescence images beyond the diffraction limit. MSSR works on low- and high-density fluorophore images, is not limited by the architecture of the detector (EM-CCD, sCMOS, or photomultiplier-based laser scanning systems) and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image series, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other analytical super resolution image approaches. Altogether, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.
Eugenol and Its Liposomal Based Nano-carrier Protects Against Anxiety Disorder in Mice.
Authors: siyal fahad jibran , siddiqui rehan ahmed , memon zahida , batool zahra , Rahman aneela Atta Ur , humayun arsalan 
Published: October 2021

Abstract
Background: Anxiety can also be termed as excessive worry which last for at least more than six months and patients can have difficulty in controlling them. Anxiety is having 31% of life time prevalence and sad part is that the anxiety disorders are not treated and diagnosed well. 3.1% which is 6.8 Million peoples of America are having anxiety disorders. The main part of clove oil is eugenol along with caryophyllene. Eugenol is present at about 70 to 90 percentage and also it contains 15 percentage of total dry weight of bud. Methodology: Pre-clinical experimental study was conducted for 10-12 Months. Male BALB/c mice were purchased from ICCBS. Sample Size was 42 BALB/c mice and divided into 07 groups. Results: The animal model was developed and it was found that Eugenol and its liposomal based nano carrier protect anxiety disorders in mice with the help of behavioral tests such as Elevated plus maze and open field tests and was recorded as Mean and SD, frequency and percentages accordingly. Conclusion: Therefore with the help of behavioral studies i.e. open field test and elevated plus maze test, eugenol and its liposomal based nano particle protect anxiety disorder in the mice by increasing the entry time and stay of mice in open arms for longer duration in elevated maze plus test and in open field the anxiety was reduced after mice spent most of time in the center rather than periphery.
Leveraging interindividual variability of regulatory activity refines genetic regulation of gene expression in schizophrenia
Authors: Alver Maris , Lykoskoufis Nikolaos , Ramisch Anna , Ongen Halit , Dermitzakis Emmanouil 
Published: October 2021

Abstract
Schizophrenia is a polygenic psychiatric disorder with limited understanding about the mechanistic changes in gene expression regulation. To elucidate on this, we integrate interindividual variability of regulatory activity with gene expression and genotype data captured from the prefrontal cortex of 272 cases and controls. We show that regulatory element activity is structured into 10,936 and 10,376 cis-regulatory domains in cases and controls, respectively, which display distinct regulatory element coordination structures in both states. By studying the interplay among genetic variants, gene expression and cis-regulatory domains, we ascertain that changes in coordinated regulatory activity tag alterations in gene expression levels (p=8.62e-06, OR=1.60), unveil case-specific QTL effects, and identify regulatory machinery changes for genes affecting synaptic function and dendritic spine morphology in schizophrenia. Altogether, we show that accounting for coordinated regulatory activity provides a novel mechanistic approach to reduce the search space for unveiling genetically perturbed regulation of gene expression in schizophrenia.
The Natural Antioxidants Curcumin and Silymarin Afforded Hepatoprotective Effects against Deferasirox-Induced Hepatotoxicity
Authors: Osman Enass Y. , Abo-Elnasr Souzan E. , Zaher Shaimaa Mohammed , AbuoHashish Norhan Ahmed 
Published: October 2021

Abstract
Deferasirox belongs to a new is a bishydroxyphenyltriazoles iron chelator used for treatment of chronic iron overload. The use of Deferasirox is associated with hepatotoxicity. Silymarin is a benzo gamma-pyrones flavonoid which affords hepatoprotection and preserves hepatocyte membranes by its antioxidant effect. Curcumin is a poly-phenol compound naturally concentrated in the herb Curcuma longa. The present study was conducted to evaluate and compare the hepatoprotective effects of both silymarin and curcumin against deferasirox-induced hepatotoxicity in rats and to explore the potential mechanisms account for their hepatoprotective effects. The present study was carried out using 32 male wistar randomly assigned into 4 groups (8 rats each) as follows; Group1: served as normal control group in which rats received distilled water (0.5ml per rat) by oral gavage. Group II (Deferasirox group) in which hepatotoxicity was induced by oral administration of deferasirox in a dose of 100 mg/kg once daily for 4 weeks dissolved in distilled water for a concentration of 35mg/ml. Group III (deferasirox+ curcumin) in which rats received curcumin in a dose of 100 mg/kg daily dose by oral gavage for 4 weeks suspended in distilled water for a concentration of 35 mg/ml one hour before administration of deferasirox. Group IV (deferasirox+ silymarin) in which rats received silymarin in dose of 7.56 mg/kg once daily by oral gavage for 4 weeks suspended in distilled water for a concentration of 2.7mg/ml one hour before administration of deferasirox. At the end of the treatment period, all rats were sacrificed by cervical dislocation, blood samples were collected for measurement of ALT, AST, ALP and total bilirubin. Liver samples were used for measurement of MDA, GSH and IL-6. Histopathological and immunohistochemistry were also done. The present data revealed that rats pretreated with curcumin or silymarin exhibited significant reduction in ALT, AST, ALP, total bilirubin, IL-6 and MDA levels with significant elevation of GSH level compared to deferasirox group. In-between group comparison revealed that there was a more significant reduction in ALT, AST, ALP, total bilirubin, IL-6 and MDA in group IV compared to group III. In conclusion, both curcumin and silymarin represent natural protective agents against Deferasirox-induced hepatotoxicity due to their antioxidant and anti-inflammatory effects.
Bibliometric Analysis of Circular RNA Research on Stem Cells: A Swiftly Expanding and Shifting Focus
Authors: Liu Shiyi , Wan Longyu , Zhang Xiaohan , Long Fei , lin changwei 
Published: October 2021

Abstract
Background: The important roles of circular RNA in various aspects of the stem cell field have been gradually reported. This is the first time in recent decades that quantitative and qualitative evidence from bibliometrics has been used to conduct an in-depth analysis of circRNA in stem cell research to reveal relative research progress, trends and hotspots.Methods: Publications from 1999 to August 7, 2021 were retrieved from the Web of Science Core Collection (WoSCC). All statistical analyses were performed using VOSviewer, CiteSpace and GraphPad Prism 7.Results: The study shows that the number of papers on circRNA in stem cells has increased significantly since 2016. Among all types of papers, articles are the most published paper types, followed by reviews. Worldwide, published papers come mostly from China, followed by the USA, Germany, and Italy. However, Israel and Iran ranked first per million people and per trillion gross domestic product (GDP), respectively. The keyword analysis results were stratified into four clusters by VOSviewer software: Cluster 1, “cancer treatment”; Cluster 2, “function and effect”; Cluster 3, “characteristics” and Cluster 4, “osteogenic differentiation”.Conclusions: Our results suggest that attention should be drawn to the latest hotspots, such as extracellular vesicles, ceRNAs and osteogenesis. In addition, suggestions on research directions and investment trends are provided to researchers and institutions.
Sterilization of Paper During Crisis
Authors: Alshammari Fwzah H. , Hussein Hebat-Allah A. 
Published: October 2021

Abstract
Paper sheets represent one of the infection risk sources inside many institutions. The present study aimed to validate the sterilization efficiency of contaminated paper sheets with different indicator pathogens by gamma radiation or dry heat technique with retaining their structure. The results show that gamma irradiation at 6 kGy, 12 kGy, and 24 kGy can successfully kill Gram-positive bacteria like Bacillus cereus and Staphylococcus aureus, Gram-negative bacteria like Escherichia Coli and Salmonella typhi, and fungi such as Candida albicans. Moreover, dry heat at 100°C for 1 hour, 150 for 30 min, or 200 for 15 min can be successful in paper decontamination completely all tested species. Surprise, micrographs of scanning electron microscope (SEM) proved that a radiation dose of 6 kGy, dry heat at 100°C for 1 hour, 150 for 30 min or 200 for 15 min is suitable for paper sheets sterilization with maintaining their structure. Ultimately, dry heat or γ-radiation as simple, useful, effective, fast, safe, and inexpensive techniques. It may be used as a precautionary step inside educational institutions especially, during written examinations period to ensure safe life for academic members during biological pandemics like covid-19.