bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate Loading of Four Guided Unsplinted Narrow Implants Supporting a Maxillary Overdenture: A Minimally Invasive Approach
Authors: Agusto Michele , Kordusky Benjamin , Bryington Matthew S. , Salman Arif , Schincaglia Gian Pietro 
Published: January 2021

IntroductionLittle evidence is available on the loading protocols for implant‐supported overdentures in the treatment of the edentulous maxilla. There are only a few case‐reports available to document the feasibility of immediate loading for maxillary overdenture, and the majority of the publications include the use of splinted implants. This paper presents a case report of immediately loaded narrow diameter implants supporting a maxillary overdenture using Locator abutments.Case PresentationA 64‐year‐old, fully edentulous patient presented with a maxillary complete removable denture. A dual cone beam computed tomography scan was obtained, and the surgical planning was performed. Four 3.3 mm diameter implants were placed at the lateral incisor and second premolar positions, using a guided and flapless approach. Locator abutments were placed, and the denture was connected immediately. The patient was instructed not to remove the prosthesis for 1 week and to follow a soft diet regimen for 2 weeks. Clinical and radiographic evaluation showed positive results at 12 months, with neither prosthetic nor implant complications.ConclusionThis is a proof of principle case report, which demonstrates the possibility of immediately loading four guided narrow diameter implants supporting a maxillary overdenture using a Locator retention system.
Comparative Study of Chemical Composition, Cholinesterase Inhibition and Antioxidant Potential of Mentha pulegium L. Essential Oil
Authors: Bektašević Mejra , Politeo Olivera , Carev Ivana 
Published: January 2021

AbstractPerennial plant Mentha pulegium L. (pennyroyal, Lamiaceae) can be found in Europe and Mediterranean. In areas where it thrives, M. pulegium is used in nutrition and as medicinal plant. Essential oil of M. pulegium is also a frequent constituent of foods and fragrances, because of mint‐like odor. Regarding the use of M. pulegium in traditional medicine and nutrition, as well as fact that essential oils are potential sources of bioactive components, this study was conducted to examine the chemical composition of essential oil of M. pulegium wild growing in Bosnia and Herzegovina and its biological activity. The chemical profile testing was made using GC/MS and GC/FID technique. Potential of cholinesterase inhibition was tested by Ellman's assay. The antioxidant activity was tested by DPPH and FRAP assay. The dominant components in analyzed oil were pulegone 54.4 %, p‐menthone 14.0 % and piperitenone 12.8 %. Good antioxidant activity and moderate cholinesterase inhibition potential of tested essential oil indicates to possibility of its use in treatment of diseases related to free radicals, Alzheimer disease and as lipid protecting antioxidant.
Validation of common reference genes stability in exosomal mRNA‐isolated from liver and breast cancer cell lines
Authors: Gorji‐Bahri Gilar , Moradtabrizi Niloofar , Vakhshiteh Faezeh , Hashemi Atieh 
Published: January 2021

AbstractAccurate relative gene expression analysis by reverse transcription‐quantitative polymerase chain reaction relies on the usage of suitable reference genes for data normalization. The RNA content of small extracellular vesicles including exosomes is growingly considered as cancer biomarkers. So, reliable relative quantification of exosomal messenger RNA (mRNA) is essential for cancer diagnosis and prognosis applications. However, suitable reference genes for accurate normalization of a target gene in exosomes derived from cancer cells are not depicted yet. Here, we analyzed the expression and stability of eight well‐known reference genes namely GAPDH, B2M, HPRT1, ACTB, YWHAZ, UBC, RNA18S, and TBP in exosomes‐isolated from the liver (Huh7, HepG2, PLC/PRF/5) and breast (SK‐BR‐3) cancer cell lines using five different algorithms including geNorm, BestKeeper, Delta Ct, NormFinder, and RefFinder. Our results showed that ACTB, TBP, and HPRT1 were not expressed in exosomes‐isolated from studied liver and breast cancer cell lines. The geNorm and BestKeeper algorithms indicated GAPDH and UBC as the most stable candidates. Moreover, Delta Ct and NormFinder algorithms showed YWHAZ as the most stable reference genes. Comprehensive ranking calculated by the RefFinder algorithm also pointed out GAPDH, YWHAZ, and UBC as the first three stable reference genes. Taken together, this study validated the common reference genes stability in exosomal mRNA derived from liver and breast cancer cell lines for the first time. We believe that this study would be the first step in finding more stable reference genes in exosomes that triggers more accurate detection of exosomal biomarkers.
Abrogation of histone deacetylases (HDACs) decreases survival of chronic myeloid leukemia cells: New insight into attenuating effects of the PI3K/c‐Myc axis on panobinostat cytotoxicity
Authors: Zehtabcheh Sara , Yousefi Amir‐Mohammad , Salari Sina , Safa Majid , Momeny Majid , Ghaffari Seyed H. , Bashash Davood 
Published: January 2021

AbstractAlthough the identification of tyrosine kinase inhibitors (TKIs) has changed the treatment paradigm of many cancer types including chronic myeloid leukemia (CML), still adjustment of neoplastic cells to cytotoxic effects of anticancer drugs is a serious challenge. In the area of drug resistance, epigenetic alterations are at the center of attention and the present study aimed to evaluate whether blockage of epigenetics mechanisms using a pan‐histone deacetylase (HDAC) inhibitor induces cell death in CML‐derived K562 cells. We found that the abrogation of HDACs using panobinostat resulted in a reduction in survival of the K562 cell line through p27‐mediated cell cycle arrest. Noteworthy, the results of the synergistic experiments revealed that HDAC suppression could be recruited as a way to potentiate cytotoxicity of Imatinib and to enhance the therapeutic efficacy of CML. Here, we proposed for the first time that the inhibitory effect of panobinostat was overshadowed, at least partially, through the aberrant activation of the phosphoinositide 3‐kinase (PI3K)/c‐Myc axis. Meanwhile, we found that upon blockage of autophagy and the proteasome pathway, as the main axis involved in the activation of autophagy, the anti‐leukemic property of the HDAC inhibitor was potentiated. Taken together, our study suggests the beneficial application of HDAC inhibition in the treatment strategies of CML; however, further in vivo studies are needed to determine the efficacy of this inhibitor, either as a single agent or in combination with small molecule inhibitors of PI3K and/or c‐Myc in this malignancy.
Successful treatment of IgG4‐related hypertrophic pachymeningitis with induction rituximab and dexamethasone followed by maintenance rituximab
Authors: Seegobin Karan , Moustafa Muhamad A. , Gannon Nicole , Keller Katelyn , Hastings Jacquelyn , Gupta Vivek , Tun Han W. , Jiang Liuyan 
Published: January 2021

AbstractIgG4‐related disease (IgG4RD) with intracranial involvement is rare. We report a 56‐year‐old male who had an excellent response to rituximab and dexamethasone after going undiagnosed for 5 years. After 3 years of rituximab maintenance, he has no evidence of disease on brain MRI.
Two new reported cases of 16q22.3q23.3 duplication syndrome highlight intrafamilial variability and potential sex expression differences within a rare duplication syndrome
Authors: Gunther Kathryn , Mowrey Kate , Farach Laura Schoch 
Published: January 2021

AbstractTwo new cases of 16q22.3q23.3 Duplication syndrome demonstrate that phenotype can vary from severely affected to mild psychiatric concerns, even within the same family and identical duplications.
Na6MQ4 (M=Zn, Cd; Q=S, Se): Promising New Ternary Infrared Nonlinear Optical Materials
Authors: Huang Yi , Gao Le , Yu HaoHai , Yang Zhihua , Li Junjie , Pan Shilie 
Published: January 2021

AbstractFour sodium‐based ternary IR nonlinear optical (NLO) materials, Na6MQ4 (M=Zn, Cd; Q=S, Se), were prepared through a high‐temperature flux method. The crystal structure of the compounds is built up of isolated [MQ4] tetrahedra and a 3D framework formed by the NaQn (n=4, 5) units. The two selenides, Na6MSe4 (M=Zn, Cd), as promising IR NLO materials, show moderate second‐harmonic generation (SHG) responses (0.9 and 0.5×AgGaS2) with good phase‐matching behavior, as well as high laser damage thresholds (2 and 1.9×AgGaS2). The two sulfides, Na6MS4 (M=Zn, Cd), exhibit higher laser damage thresholds (13 and 4×AgGaS2), but smaller SHG responses (0.3 and 0.2×AgGaS2). Theoretical calculations and statistical analyses indicate that the SHG effect and band gap in the compounds originate mainly from the distorted NaQ4 NLO‐active units with a short Na−S bond length, which provides a new insight into the design of novel IR NLO materials.
Directed Design of a AuI Complex with a Reduced Mesoionic Carbene Radical Ligand: Insights from 1,2,3‐Triazolylidene Selenium Adducts and Extensive Electrochemical Investigations
Authors: Beerhues Julia , Neubrand Maren , Sobottka Sebastian , Neuman Nicolás I. , Aberhan Hannes , Chandra Shubhadeep , Sarkar Biprajit 
Published: January 2021

AbstractCarbene‐based radicals are important for both fundamental and applied chemical research. Herein, extensive electrochemical investigations of nine different 1,2,3‐triazolylidene selenium adducts are reported. It is found that the half‐wave potentials of the first reduction of the selones correlate with their calculated LUMO levels and the LUMO levels of the corresponding triazolylidene‐based mesoionic carbenes (MICs). Furthermore, unexpected quasi‐reversibility of the reduction of two triazoline selones, exhibiting comparable reduction potentials, was discovered. Through UV/Vis/NIR and EPR spectroelectrochemical investigations supported by DFT calculations, the radical anion was unambiguously assigned to be triazoline centered. This electrochemical behavior was transferred to a triazolylidene‐type MIC‐gold phenyl complex resulting in a MIC‐radical coordinated AuI species. Apart from UV‐Vis‐NIR and EPR spectroelectrochemical investigations of the reduction, the reduced gold‐coordinated MIC radical complex was also formed in situ in the bulk through chemical reduction. This is the first report of a monodentate triazolylidene‐based MIC ligand that can be reduced to its anion radical in a metal complex. The results presented here provide design principles for stabilizing radicals based on MICs.
Regioselective Synthesis of 1,2,3,4‐Tetrasubstituted Arenes by Vicinal Functionalization of Arynes Derived from Aryl(Mes)iodonium Salts**
Authors: Nilova Aleksandra , Sibbald Paul A. , Valente Edward J. , González‐Montiel Gisela A. , Richardson H. Camille , Brown Kevin S. , Cheong Paul Ha‐Yeon , Stuart David R. 
Published: January 2021

AbstractHerein, the synthesis of 1,2,3,4‐tetrasubstituted benzenoid rings, motifs found in pharmaceutical, agrochemical, and natural products, is described.[1] In the past, the regioselective syntheses of such compounds have been a significant challenge. This work reports a method using substituted arynes derived from aryl(Mes)iodonium salts to access a range of densely functionalized 1,2,3,4‐tetrasubstituted benzenoid rings. Significantly, it was found that halide substituents are compatible under these conditions, enabling post‐synthetic elaboration via palladium‐catalyzed coupling. This concise strategy is predicated on two regioselective events: 1) ortho‐ deprotonation of aryl(Mes)iodonium salts to generate a substituted aryne intermediate, and 2) regioselective trapping of said arynes, thereby improving previously reported reaction conditions to generate arynes at room temperature and in shorter reaction times. Density functional theory (DFT) computations and linear free energy relationship (LFER) analysis suggest the regioselectivity of deprotonation is influenced by both proximal and distal ring substituents on the aryne precursor. A competition experiment further reveals the role of arene substituents on relative reactivity of aryl(Mes)iodoniums as aryne precursors.
Synthesis of 3‐Methylthioindolesvia Intramolecular Cyclization of 2‐Alkynylanilines Mediated by DMSO/DMSO‐d6 and SOCl2
Authors: Li Xuemin , Zhang Beibei , Zhang Jingran , Wang Xi , Zhang Dongke , Du Yunfei , Zhao Kang 
Published: January 2021

Main observation and conclusionThe intramolecular cyclization of 2‐alkynylanilines mediated by DMSO/SOCl2 was found to afford biologically interesting 3‐methylthioindoles, which are rarely obtained by the exiting methods. DMSO could also be replaced with its deuterated counterpart, enabling the method applicable to the construction of indole skeleton bearing a SCD3 moiety at its 3‐position.
Novel germline TRAF3IP3 mutation in a dyad with familial acute B lymphoblastic leukemia
Authors: Pommert Lauren , Burns Robert , Furumo Quinlan , Pulakanti Kirthi , Brandt Jon , Burke Michael J. , Rao Sridhar 
Published: January 2021

AbstractBackgroundAcute lymphoblastic leukemia (ALL) is the most common hematologic malignancy in children, representing 25% of all new cancer diagnoses. Advances in genomic sequencing have demonstrated that inherited genetic risk factors play a larger role in leukemia development than previously appreciated.AimWe identified a father–daughter dyad with childhood B‐cell ALL and aimed to investigate whether the pair shared a gene associated with leukemia predisposition.MethodsWe performed whole exome sequencing on their leukemia and germline samples and RNA‐seq on their leukemia samples.ResultsWe discovered a novel germline chromosomal structural variant in chromosome 1q32.2 within the TRAF3IP3 gene. TRAF3IP3 regulates B‐cell lymphopoiesis, and this mutation likely resulted in a predisposition to leukemia by causing expansion of immature B‐cell precursors which are highly vulnerable to secondary somatic mutations. Based on the lack of concordance in the somatic mutational profiles between this dyad's leukemia samples, we suspect that the acquired somatic mutations rather than this germline mutation are what dictated their leukemia phenotypes, which we confirmed through RNA‐seq by comparing to sporadic cases of B‐cell ALL.ConclusionThis research may have identified a novel gene involved in leukemogenesis which may also be involved in de novo cases of ALL. Additional studies are needed to further characterize this TRAF3IP3 structural variant, the co‐occurring somatic mutations within these leukemia samples and their combined role in leukemogenesis.
Chemical Synthesis and Substrate Temperature Effect on Morphology of 2D Vanadium Disulfide
Authors: Hossain Mongur , Qin Biao , Sen Sapan Kumar 
Published: January 2021

Abstract2D metallic transition metal dichalcogenides exhibit plenty of intriguing physical properties such as magnetism, charge density wave, and superconductivity resulting in worldwide attention from condensed‐matter physicists over the last few decades. Herein, the chemical synthesis and substrate temperature effect on the morphology of 2D vanadium disulfide (VS2) are reported. The synthesis is done by atmospheric pressure chemical vapor deposition using vanadium chloride (VCl3) precursor on SiO2/Si, fluorphlogopite mica, and hexagonal boron nitride (h‐BN) substrates. The effect of substrate temperatures on the morphology of 2D VS2 nanosheets growth is investigated by optical microscopy, scanning electron microscopy, and atomic force microscopy. Large area VS2 films are attained when the substrate temperature is 650 °C whereas VS2 single crystals are obtained at 550 °C.
Molecular similarity between the mechanisms of epithelial fusion and fetal wound healing during the closure of the caudal neural tube in mouse embryos
Authors: Fernández‐Santos Beatriz , Caro‐Vega José Manuel , Sola‐Idígora Noelia , Lazarini‐Suárez Cecilia , Mañas‐García Laura , Duarte Patrícia , Fuerte‐Hortigón Alejandro , Ybot‐González Patricia 
Published: January 2021

AbstractBackgroundNeural tube (NT) closure is a complex developmental process that takes place in the early stages of embryogenesis and that is a key step in neurulation. In mammals, the process by which the neural plate generates the NT requires organized cell movements and tissue folding, and it terminates with the fusion of the apposed ends of the neural folds.ResultsHere we describe how almost identical cellular and molecular machinery is used to fuse the spinal neural folds as that involved in the repair of epithelial injury in the same area of the embryo. For both natural and wound activated closure of caudal neural tissue, hyaluronic acid and platelet‐derived growth factor signaling appear to be crucial for the final fusion step.ConclusionsThere seems to be no general wound healing machinery for all tissues but rather, a tissue‐specific epithelial fusion machinery that embryos activate when necessary after abnormal epithelial opening.
Bridging executive function and disinhibited eating among youth: A network analysis
Authors: Byrne Meghan E. , Tanofsky‐Kraff Marian , Lavender Jason M. , Parker Megan N. , Shank Lisa M. , Swanson Taylor N. , Ramirez Eliana , LeMay‐Russell Sarah , Yang Shanna B. , Brady Sheila M. , Zenno Anna , Chivukula Krishna Karthik , Kelly Nichole R. , Yanovski Jack A. 
Published: January 2021

AbstractObjectivePoorer executive function (EF) has been linked to disinhibited eating in youth, suggesting poor EF predisposes toward obesity, yet the specific nature and extent of interconnections between facets of these domains is unclear. Network analysis provides a promising framework for elucidating the relationship between poor EF and disinhibited eating, and offers insights into potential maintenance processes.MethodAmong youth ages 8–17 years, a regularized partial correlation network of EF and disinhibited eating facets was estimated to examine expected influence centrality and bridge expected influence. Computerized neurocognitive tasks assessed EF variables, including decision‐making, general and food‐related inhibitory control, delayed gratification, cognitive flexibility, and working memory. Disinhibited eating variables included total carbohydrate–fat intake at a laboratory test meal and self‐reported eating in the absence of hunger, emotional eating, and loss‐of‐control eating severity.ResultsIn the current sample (N = 248; Mage = 12.5; 54.8% female; 43.5% non‐Hispanic White; 25.8% non‐Hispanic Black; BMI %ile = 65.8 ± 27.8), emotional eating in response to depressive symptoms emerged as a central symptom in the network. Carbohydrate–fat intake had the highest bridge expected influence and was most strongly connected to general inhibitory control (part r = .14).DiscussionThe link between general inhibitory control and objective palatable food intake may be particularly salient in maintaining maladaptive eating behavior. Interventions targeting behavioral disinhibition may disrupt associations among a network of disinhibited eating facets in youth and should be targets for longitudinal research.
Worsening of heart failure by coronavirus disease 2019 is associated with high mortality
Authors: Bocchi Edimar Alcides , Lima Ivna Girard Cunha Vieira , Biselli Bruno , Salemi Vera Maria Cury , Ferreira Silvia Moreira Ayub , Chizzola Paulo Roberto , Munhoz Robinson Tadeu , Pessoa Ranna Santos , Cardoso Francisco Akira Malta , Bello Mariana Vieira de Oliveira , Hajjar Ludhmila Abrahão , Gomes Brenno Rizerio 
Published: January 2021

AbstractAimsPatients with advanced heart failure (HF) with reduced left ventricular ejection fraction (HFrEF) and concurrent coronavirus disease 2019 (COVID‐19) might have a higher risk of severe events.Methods and resultsWe retrospectively studied 16 patients with advanced HFrEF who developed COVID‐19 between 1 March and 29 May 2020. Follow‐up lasted until 30 September. Ten patients previously hospitalized with decompensated HFrEF were infected with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) during hospitalization. Six patients undergoing ambulatory care at initiation of COVID‐19 symptoms were hospitalized because of advanced HFrEF. All patients who experienced worsening of HFrEF due to COVID‐19 required higher doses or introduction of additional inotropic drugs or intra‐aortic balloon pump in the intensive care unit. The mean intravenous dobutamine dose before SARS‐CoV‐2 infection in previously hospitalized patients (n = 10) and the median (inter‐quartile range) peak intravenous dobutamine dose during SARS‐CoV‐2 infection in all patients (n = 16) were 2 (0–7) μg/kg/min and 20 (14–20) (P < 0.001), respectively. During follow‐up, 56% underwent heart transplantation (n = 2) or died (n = 7). Four patients died during hospitalization from mixed shock consequent to severe acute respiratory syndrome with inflammatory storm syndrome associated with septic and cardiogenic shock during COVID‐19. After COVID‐19 recovery, two patients died from mixed septic and cardiogenic shock and one from sustained ventricular tachycardia and cardiogenic shock. Five patients were discharged from hospital to ambulatory care. Four were awaiting heart transplantation.ConclusionWorsening of advanced HF by COVID‐19 is associated with high mortality. This report highlights the importance of preventing COVID‐19 in patients with advanced HF.
High‐protein vs. standard‐protein diets in overweight and obese patients with heart failure and diabetes mellitus: findings of the Pro‐HEART trial
Authors: Evangelista Lorraine S. , Jose Mini M. , Sallam Hanaa , Serag Hani , Golovko George , Khanipov Kamil , Hamilton Michele A. , Fonarow Gregg C. 
Published: January 2021

AbstractAimsThe intermediate‐term effects of dietary protein on cardiometabolic risk factors in overweight and obese patients with heart failure and diabetes mellitus are unknown. We compared the effect of two calorie‐restricted diets on cardiometabolic risk factors in this population.Methods and resultsIn this randomized controlled study, 76 overweight and obese (mean weight, 107.8 ± 20.8 kg) patients aged 57.7 ± 9.7 years, 72.4% male, were randomized to a high‐protein (30% protein, 40% carbohydrates, and 30% fat) or standard‐protein diet (15% protein, 55% carbohydrates, and 30% fat) for 3 months. Reductions in weight and cardiometabolic risks were evaluated at 3 months. Both diets were equally effective in reducing weight (3.6 vs. 2.9 kg) and waist circumference (1.9 vs. 1.3 cm), but the high‐protein diet decreased to a greater extent glycosylated haemoglobin levels (0.7% vs. 0.1%, P = 0.002), cholesterol (16.8 vs. 0.9 mg/dL, P = 0.031), and triglyceride (25.7 vs. 5.7 mg/dL, P = 0.032), when compared with the standard‐protein diet. The high‐protein diet also significantly improved both systolic and diastolic blood pressure than the standard‐protein diet (P < 0.001 and P = 0.040, respectively).ConclusionsBoth energy‐restricted diets reduced weight and visceral fat. However, the high‐protein diet resulted in greater reductions in cardiometabolic risks relative to a standard‐protein diet. These results suggest that a high‐protein diet may be more effective in reducing cardiometabolic risk in this population, but further trials of longer duration are needed.