bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of preoperative plasma suPAR levels with intraoperative sublingual microvascular perfusion in patients undergoing major non-cardiac surgery
Authors: Chalkias Athanasios , Papagiannakis Nikolaos , Saugel Bernd , Flick Moritz , Kolonia Konstantina , Angelopoulou Zacharoula , Ragias Dimitrios , Papaspyrou Dimitra , Ntalarizou Nicoletta , Bouzia Aikaterini , Stamoulis Konstantinos , Kyriakaki Aikaterini , Eugen-Olsen Jesper , Laou Eleni , Arnaoutoglou Eleni 
Published: July 2021

ABSTRACTINTRODUCTIONThe plasma suPAR level has previously been associated with postoperative complications and has been shown to be an independent predictor of coronary microvascular function and flow reserve. We investigated the association between preoperative suPAR levels and intraoperative sublingual microvascular perfusion in patients undergoing elective major non-cardiac surgery.MethodsThis study included 100 patients undergoing major non-cardiac surgery between February 2019 and September 2020. The primary objective was to investigate the association between preoperative suPAR and intraoperative sublingual De Backer score, Consensus Proportion of Perfused Vessels (Consensus PPV), and Consensus PPV (small). Secondary objectives were to investigate the associations between these sublingual microcirculatory variables and (1) complications and (2) mean arterial pressure. EDTA blood was collected before induction of anesthesia and plasma suPAR levels were determined using the suPARnostic® quick triage lateral flow assay. Sublingual microcirculation was monitored with Sidestream DarkField (SDF+) imaging technique at 20 minutes after induction of anesthesia before surgical incision (baseline) and then every 30 minutes until emergence from anesthesia.ResultsA decrease of 0.7 mm-1 in the De Backer score, 2.5% in the Consensus PPV, and 2.8% in the Consensus PPV (small) from baseline measurement was observed for every 1 ng/ml increase of suPAR or 1 additional minute of intraoperative time. De Baker score did not change significantly from baseline (p=0.404), while Consensus PPV and Consensus PPV (small) decreased significantly from baseline (p<0.001 in both cases). The De Backer score, the Consensus PPV, and the Consensus PPV (small) correlated with postoperative complications. Mean arterial pressure correlated with De Backer score (p=0.487) but not with Consensus PPV (p=0.506) or Consensus PPV (small) (p=0.697) during the intraoperative period.ConclusionPreoperative suPAR levels and prolonged operative duration were associated with intraoperative impairment of sublingual microvascular perfusion in patients undergoing elective major non-cardiac surgery.
PHENOTYPIC CHARACTERIZATION OF TWO NOVEL CELL LINE MODELS OF CASTRATION RESISTANT PROSTATE CANCER
Authors: Haffner Michael C. , Bhamidipati Akshay , Tsai Harrison K. , Esopi David M. , Vaghasia Ajay M. , Low Jin-Yih , Patel Radhika A. , Guner Gunes , Pham Minh-Tam , Castagna Nicole , Hicks Jessica , Wyhs Nicolas , Aebersold Ruedi , De Marzo Angelo M. , Nelson William G. , Guo Tiannan , Yegnasubramanian Srinivasan 
Published: July 2021

ABSTRACTBACKGROUNDResistance to androgen deprivation therapies is a major driver of mortality in advanced prostate cancer. Therefore, there is a need to develop new pre-clinical models that allow the investigation of resistance mechanisms and the assessment of drugs for the treatment of castration resistant prostate cancer.METHODSWe generated two novel cell line models (LAPC4-CR and VCaP-CR) which were derived by passaging LAPC4 and VCaP cells in vivo and in vitro under castrate conditions. We performed detailed transcriptomic (RNA-seq) and proteomic analyses (SWATH-MS) to delineate expression differences between castration-sensitive and castration-resistant cell lines. Furthermore, we characterized the in vivo and in vitro growth characteristics of the novel cell line models.RESULTSThe two cell line derivatives LAPC4-CR and VCaP-CR showed castration resistant growth in vitro and in vivo which was only minimally inhibited by AR antagonists, enzalutamide and bicalutamide. High-dose androgen treatment resulted in significant growth arrest of VCaP-CR but not in LAPC4-CR cells. Both cell lines maintained AR expression, but exhibited distinct expression changes on the mRNA and protein level. Integrated analyses including data from LNCaP and the previously described castration resistant LNCaP-abl cells revealed an expression signature of castration resistance.CONCLUSIONSThe two novel cell line models LAPC4-CR and VCaP-CR and their comprehensive characterization on the RNA and protein level represent important resources to study the molecular mechanisms of castration resistance.
ECM-integrin signalling instructs cellular position-sensing to pattern the early mouse embryo
Authors: Kim Esther Jeong Yoon , Sorokin Lydia , Hiiragi Takashi 
Published: July 2021

SummaryDevelopment entails patterned emergence of diverse cell types within the embryo. In mammals, cells positioned inside the embryo gives rise to the inner cell mass (ICM) that eventually forms the embryo proper. Yet the molecular basis of how these cells recognise their ‘inside’ position to instruct their fate is unknown. Here we show that cells perceive their position through extracellular matrix (ECM) and integrin-mediated adhesion. Provision of ECM to isolated embryonic cells induces ICM specification and alters subsequent spatial arrangement between epiblast (EPI) and primitive endoderm (PrE) cells that emerge within the ICM. Notably, this effect is dependent on integrin β1 activity and involves apical to basal conversion of cell polarity. We demonstrate that ECM-integrin activity is sufficient for ‘inside’ positional signalling and it is required for proper sorting of EPI/PrE cells. Our findings thus highlight the significance of ECM-integrin adhesion in enabling position-sensing by cells to achieve tissue patterning.
Coding of interceptive saccades in parietal cortex of macaque monkeys
Authors: Churan Jan , Kaminiarz Andre , Schwenk Jakob C. B. , Bremmer Frank 
Published: July 2021

AbstractThe oculomotor system can initiate remarkably accurate saccades towards moving targets (interceptive saccades) the processing of which is still under debate. The generation of these saccades requires the oculomotor centers to have information about the motion parameters of the target that then must be extrapolated to bridge the inherent processing delays. We investigated to what degree the information about motion of a saccade target is available in the lateral intra-parietal area (area LIP) of macaque monkeys for generation of accurate interceptive saccades. When a multi-layer neural network was trained based on neural discharges from area LIP around the time of saccades towards stationary targets it was also able to predict the end points of saccades directed towards moving targets. This prediction, however, lagged behind the actual post-saccadic position of the moving target by ~80 ms when the whole neuronal sample of 105 neurons was used. We further found that single neurons differentially code for the motion of the target. Selecting neurons with the strongest representation of target motion reduced this lag to ~30 ms which represents the position of the moving target approximately at the onset of the interceptive saccade. We conclude that - similarly to recent findings from the Superior Colliculus (Goffart et al., 2017) – there is a continuum of contributions of individual LIP neurons to the accuracy of interceptive saccades. A contribution of other gaze control centers (like the cerebellum or the frontal eye field) that further increase the saccadic accuracy is, however, likely.
A new transgene mouse model using an extravesicular EGFP tag to elucidate the in vivo function of extracellular vesicles
Authors: Ø. Nørgård Mikkel , Steffensen Lasse B. , Hansen Didde R. , Füchtbauer Ernst-Martin , Engelund Morten B. , Dimke Henrik , Jensen Boye L. , Andersen Ditte C. , Svenningsen Per 
Published: July 2021

AbstractThe in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since cell-specific EVs are difficult to isolate. We therefore created an EV reporter using CD9 to display enhanced green fluorescent protein (EGFP) on the EV surface. CD9-EGFP expression in cells did not affect EV size and concentration, but allowed for co-precipitation of EV markers TSG101 and ALIX from cell-conditioned medium by anti-GFP immunoprecipitation. We created a transgenic mouse where CD9-EGFP was inserted in the inverse orientation and double-floxed, ensuring Cre recombinase-dependent EV reporter expression. We crossed the EV reporter mice with mice expressing Cre ubiquitously (CMV- Cre), in cardiomyocytes (AMHC-Cre) and kidney epithelium (Pax8-Cre), respectively. The mice showed tissue-specific EGFP expression, and plasma and urine samples were used to immunoprecipitate EVs. CD9-EGFP EVs was detected in plasma samples from CMV-Cre/CD9-EGFP and AMHC-Cre/CD9-EGFP mice, but not in PAX8-Cre/CD9-EGFP mice. On the other hand, CD9-EGFP EVs were detected in urine samples from CMV-Cre/CD9-EGFP and PAX8-Cre/CD9-EGFP mice, but not AMHC-Cre/CD9-EGFP, indicating that plasma EVs are not filtered to the urine. In conclusion, our EV reporter mouse model enables Cre-dependent EV labeling, providing a new approach to study cell-specific EVs in vivo and gain new insight into their physiological and pathophysiological function.
An improved ellipsoid optimization algorithm in subspace predictive control
Authors: Jianhong Wang 
Published: July 2021

Purpose
The purpose of this paper is to derive the output predictor for a stationary normal process with rational spectral density and linear stochastic discrete-time state-space model, respectively, as the output predictor is very important in model predictive control. The derivations are only dependent on matrix operations. Based on the output predictor, one quadratic programming problem is constructed to achieve the goal of subspace predictive control. Then an improved ellipsoid optimization algorithm is proposed to solve the optimal control input and the complexity analysis of this improved ellipsoid optimization algorithm is also given to complete the previous work. Finally, by the example of the helicopter, the efficiency of the proposed control strategy can be easily realized.

Design/methodology/approach
First, a stationary normal process with rational spectral density and one stochastic discrete-time state-space model is described. Second, the output predictors for these two forms are derived, respectively, and the derivation processes are dependent on the Diophantine equation and some basic matrix operations. Third, after inserting these two output predictors into the cost function of predictive control, the control input can be solved by using the improved ellipsoid optimization algorithm and the complexity analysis corresponding to this improved ellipsoid optimization algorithm is also provided.

Findings
Subspace predictive control can not only enable automatically tune the parameters in predictive control but also avoids many steps in classical linear Gaussian control. It means that subspace predictive control is independent of any prior knowledge of the controller. An improved ellipsoid optimization algorithm is used to solve the optimal control input and the complexity analysis of this algorithm is also given.

Originality/value
To the best knowledge of the authors, this is the first attempt at deriving the output predictors for stationary normal processes with rational spectral density and one stochastic discrete-time state-space model. Then, the derivation processes are dependent on the Diophantine equation and some basic matrix operations. The complexity analysis corresponding to this improved ellipsoid optimization algorithm is analyzed.
Announcement of formal controls as phase-shifting perceptions: their determinants and moderating role in the context of mobile loafing
Authors: Kwak Dong-Heon , Lee Saerom , Ma Xiao , Lee Jaeung , Lara Khansa , Brandyberry Alan 
Published: July 2021

PurposeMobile loafing, or non-work-related mobile computing, is deviant workplace behavior that can reduce productivity and increase cybersecurity risks. To thwart mobile loafing, organizations often adopt formal controls that encompass rules and policies. These formal controls can serve as a phase-shifting event. Phase shifting is a process where individuals reevaluate and revise their perceptions of the regulation of deviant behaviors. Despite the importance of understanding this process, little research has examined the announcement of formal controls as an impetus for phase shifting. The primary objectives of this study were to induce a phase-shifting perception in an organizational setting and explore its determinants and moderating role in the context of mobile loafing.Design/methodology/approachThe authors proposed and tested a model using two-wave data collected from 231 Amazon Mechanical Turk workers. To test the research hypotheses, they used covariance-based structural equation modeling and logistic regression.FindingsThe authors found that peer's mobile loafing and neutralization positively influence mobile-loafing intention before and after the announcement of formal controls. This research also shows that the higher an employee's neutralization, the likelier they perceive the announcement of formal controls as phase shifting. Also, the authors found that the moderating effect of phase-shifting perceptions functions in such a way that the relationship between T1 and T2 mobile-loafing intention is weaker when employees perceive the announcement of formal controls as a phase-shifting event.Practical implicationsThe authors’ results provide managers with useful insights into effectively using formal controls to mitigate employees' deviant behavior. To effectively use formal controls, managers should articulate formal controls that can trigger employees to revise their perceptions of counterproductive workplace behavior policies.Originality/valueThis study is one of the first in information systems research to empirically examine the announcement of formal controls as a phase-shifting event and explore its antecedents and moderating role in the context of deviant workplace behavior in general and mobile loafing in particular.
A model for key success factors for outsourcing in project-based companies: integrating soft and dynamic approaches
Authors: Hoseini Delshad , Shafiei Nikabadi Mohsen 
Published: July 2021

Purpose
The purpose of this study is to achieve the dynamic model of outsourcing success factors in project-based companies.

Design/methodology/approach
This study is descriptive-survey in terms of method and practical in terms of purpose. To achieve the dynamic model of outsourcing success, 1,000 outsourcing articles published in high-status journals from 2017 to 2019 were first text-mining. Then, using the clustering technique, the factors affecting the success of outsourcing were obtained. To achieve the key variables, the variables obtained by interpretive structural modeling (ISM) were then leveled. Then, the strategic options development and analysis (SODA) technique has been used to achieve a consensus and coordination on factors relationships. Finally, the dynamic model of outsourcing success in GHODRAT CONTROL PARS Company has been modeled and implemented.

Findings
In total, five clusters and nine factors were extracted (strategy, management, performance, market, R&D, supplier, product, organizational data and outsourcing findings). In central and domain analysis, two factors, “Strategy” and “R and D,” were recognized as factors that have the most interaction and centrality. The result of the dynamic model indicate that the organization will significantly reduce the construction time of the power plant by improving the “R and D” factor.

Originality/value
In this study, various techniques have been combined. Therefore, one of the aspects of innovation in the present study is the combination of methods that have not been used earlier. The second aspect of this study’s innovation is using SODA technique to design the dynamic model of outsourcing success factors. Given that the scope of this study is the component affecting the success of outsourcing, so extensive research has been conducted in the field of articles worked in the field of outsourcing to get a comprehensive view of the components affecting the success of outsourcing, which has not been reviewed in other articles. In this study, in addition to identifying the effective factors, their identified and also how these variables affect the successful performance of outsourcing in the form of a dynamic model, and then analyzed.
Determinants of opportunism in Uganda’s manufacturing sector:a comparison of two sectors
Authors: Eyaa Sarah , Sridharan Ramaswami , Ryan Suzanne 
Published: July 2021

PurposeThe purpose of this paper is to propose a conceptual model investigating the impact of three constructs, environmental uncertainty, power asymmetry and information sharing on opportunism engagement in exchange relationships.Design/methodology/approachData were collected from procurement or sales managers of 99 manufacturing firms in Kampala, Uganda’s capital using a cross-sectional survey. Hypotheses were tested in both the agricultural and non-agricultural manufacturing sectors using multiple regression runs in the SPSS software.FindingsEnvironmental uncertainty increases opportunism in the agricultural sector whilst power asymmetry increases opportunism in the non-agricultural sector. Across both sectors, information sharing does not have a significant impact on opportunism.Originality/valueThis paper contributes to a deeper understanding of opportunism in a developing country context by highlighting the contextual factors within the agricultural and non-agricultural manufacturing sectors that influence opportunism engagement under conditions of environmental uncertainty, power asymmetry and information sharing. This paper presents implications for practice and policy to minimise opportunism with the goal of enhancing the participation of Ugandan manufacturing firms in global supply chains.
Exploring peer feedback on behaviour in the international classroom: a case study on students’ experiences and perceptions
Authors: Van Rompay-Bartels Ingrid , Geessink Jannemieke 
Published: July 2021

Purpose
In spite of the potential of peer feedback, research related to the international classroom and the development of intercultural competences remains limited. This paper aims to further explore this combination and associated gaps by presenting students’ perceptions of peer feedback on individual behaviour in group work.

Design/methodology/approach
Several studies have shown that peer feedback can be a powerful instrument in higher education. For this reason, this instrument is increasingly being deployed in the international classroom of a Dutch Business School (DBS), which has a student population of about 60 different nationalities. The present paper adopts an embedded case-study design in studying peer feedback within the international classroom.

Findings
The primary results of this study are twofold. First, they show that before joining DBS, the vast majority of international students have never been exposed to group work peer feedback. And second, they reveal that cultural background (bias) is a critical factor in how students provide and perceive peer feedback. Students from high-context cultures struggle with direct feedback provided by students from low-context cultures. Furthermore, the results show that domestic cultural values “lack consideration” when dealing with the contrasts in cultural values of non-domestic (international) students.

Originality/value
This study indicates that several aspects of the students’ cultural background have a direct impact on how they provide and perceive individual peer feedback on their behaviour in group work. Furthermore, it argues that peer feedback, when used as an instrument, requires specific training and guidance of students with regard to cultural differences, values and perceptions.
Process optimisation and product characterisation of milk protein concentrate extrudates expanded by supercritical carbon dioxide
Authors: Arora Bindvi , Rizvi Syed S H 
Published: July 2021

The effects of water and supercritical CO2 (SC–CO2) addition rate, and pressure at SC–CO2 injection (PCO2) on physical and rheological properties of milk protein concentrate (MPC81) and MPC81‐sucrose (MPC81‐S) extrudates were investigated. As PCO2 increased, extrudate expansion decreased and storage modulus and hardness increased. PCO2 for MPC81 and MPC81‐S was optimised as 13.78 MPa and 11.03 MPa, respectively, whereas water rate of 40 (wt. % dry feed) and SC–CO2 rate of 2.25 (wt. % dry feed) were optimised for both formulations. Elucidating the association of extrusion parameters and extrudate properties will facilitate the development of MPC extrudates of commercial utility.
AK bullet (7.62 × 39 mm) holes on 1‐mm sheet metal: A forensic‐related study in aid of bullet trajectory reconstruction
Authors: Nishshanka Bandula , Shepherd Chris , Ariyarathna Randika 
Published: July 2021

AbstractBullet holes play a major role in the trajectory determination of fired bullets, potentially suggesting where the shots were fired from. Few methods are currently employed to estimate the angles of incidence of fired bullets using bullet holes. The literature has stressed that knowledge about the impact behavior of the specific bullet–substrate interfaces, including the most viable method for estimating the angle of incidence of that combination, requires empirical testing to enable successful analysis. The perforating bullet hole characteristics of AK bullets fired into 1‐mm sheet metal over a range of angles of incidence have been documented in this study to aid bullet trajectory determination. A strong inverse relationship has been demonstrated between particular impact mark dimensions and the angles of incidence, revealing the possibility of using these dimensions to predict the angles of incidence for AK bullets perforating 1‐mm sheet metal in actual crime scenes. This study has further confirmed the existence of a recently reported phenomenon for impacts related to this projectile–substrate combination, the “double‐headed impact mark,” and relationships with the angles of incidence of fired AK bullets. While suggesting an alternative and confirmative new method to understand the angles of incidence of fired AK bullets in 1‐mm sheet metal using measured bullet defects, the study highlights a complex deviation phenomenon and a potential error that could occur when determining bullet trajectories using probing, stringing, or laser methods for the impact conditions described.