bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-invasive ML methods for diagnosis of congenital heart disease associated with pulmonary arterial hypertension
Authors: Gao Yuyang , Ma Pengyue , Pan Jiahua , Yang Hongbo , Guo Tao , Wang Weilian 
Published: January 2025

ObjectiveCongenital heart disease with pulmonary arterial hypertension (CHD-PAH), caused by CHD, is associated with high clinical mortality. Hence, timely diagnosis is imperative for treatment.ApproachTwo non-invasive diagnosis algorithms of CHD-PAH were put forward in this review, which were direct three-divided and two-stage classification models. Pre-processing in both algorithms focuses on segmentation of heart sounds into discrete cardiac cycles. Both the dual-threshold and Bi-LSTM (Bi-directional Long Short-Term Memory) methods demonstrate efficacy. In the feature extraction phase, the direct three-divided model integrate time-, frequency-, and energy-domain features with deep learning features. While the two-stage classification model sequentially extracts sub-band envelopes and short-time energy of cardiac cycle. In the classification phase, considering the lack of CHD-PAH data, ensemble learning was widely used.Main resultsAn accuracy of 88.61% was achieved with direct three-divided model and 90.9% with two-stage classification model.SignificanceBy analyzing and discussing these algorithms, future research directions of CHD-PAH assisted diagnosis were discussed. It is hoped that it will provide insight into prediction of CHD-PAH. Thus saving people from death due to untimely assistance.
Combined effect of the smartphone addiction and physical activity on the depressive symptoms in secondary school students: a cross sectional study in Shanghai, China
Authors: Yang Rui , Tan Shuoyuan , Abdukerima Gulqihra , Lu Ting , Chen Chen , Song Lixin , Ji Bing , Lv Yipeng , Shi Jianwei 
Published: January 2025

ObjectiveThis study aims to investigate the prevalence of smartphone addiction, physical activity levels, and depressive symptoms among secondary school students, and to analyze the combined impact of smartphone addiction and physical activity on depressive symptoms.MethodsA cluster sampling method was employed in two secondary schools in the Jing’an District of Shanghai, China. Univariate analysis was used to compare the prevalence of depressive symptoms across different demographic characteristics. Logistic regression was utilized to examine the associations between smartphone addiction, physical activity, and their combined effect on depressive symptoms.ResultsA total of 1,316 respondents participated in the study, with reported prevalence rates of depressive symptoms (36.2%), smartphone addiction (19.2%), and insufficient physical activity (23.3%). Risk factors for depressive symptoms included being a non-only child (OR=1.421, 95% CI: 1.090-1.853, P=0.009), inadequate sleep duration (OR=2.722, 95% CI: 2.070-3.578, P&lt;0.001) and smartphone addiction (OR=2.173, 95% CI: 1.621-2.913, P &lt; 0.001). Adolescents with smartphone addiction were significantly more likely to report depressive symptoms compared to those without (OR=2.173, 95% CI: 1.621-2.913, P &lt; 0.001). Joint analysis indicated that combined smartphone addiction and insufficient physical activity significantly increased the risk of depressive symptoms (OR=2.781, 95% CI: 1.627-4.753, P &lt; 0.001).ConclusionThe study identified a high prevalence of severe smartphone addiction, insufficient physical activity, and elevated rates of depressive symptoms among secondary school students. Smartphone addiction and inadequate physical activity were associated with increased likelihood of depressive symptoms. Moreover, higher levels of physical activity appeared to mitigate the adverse impact of smartphone addiction on depressive symptoms.
Relationship between alcohol use and overactive bladder disease: a cross-sectional study of the NHANES 2005–2016
Authors: Zhang Yixin , Qin Weijun 
Published: January 2025

BackgroundOveractive bladder syndrome (OAB) is a prevalent urological condition which has a substantial impact on the life quality of affected individuals, resulting in restrictions in daily activities and work productivity. Alcohol is a diuretic, which means that it increases urine production and can potentially worsen urinary urgency and frequency. Several studies have investigated the association between alcohol consumption and OAB symptoms, but the results have been conflicting. This study aimed to investigate the relationship between alcohol consumption and OAB symptoms using a large, nationally representative sample.MethodData from the National Health and Nutrition Examination Survey 2005–2016 were obtained for analysis. The Overactive Bladder Symptom Scale (OBSS) was used to determine the presence of OAB in each participant. Multivariate logistic regression and ordinal logistic regression were used to analyze the association of alcohol use frequency and quantity with the onset and severity of OAB, respectively.ResultsA total of 7,805 samples (representing the 1,473,525,341 US population after weighting) were included in our analysis. Approximately 12.4% of this weighted sample self-reported having OAB. A greater proportion of nondrinkers, a higher proportion of females, higher blood pressure, older age, and lower income levels were observed in OAB patients compared to non-OAB patients. Univariate logistic regression revealed that the risk of OAB was significantly greater in the nondrinker group than in the 1–5 drinks/month (OR 0.64; 95% CI, 0.50–0.83), 5–10 drinks/month (OR 0.60; 95% CI, 0.43–0.82) and 10+ drinks/month groups (OR 0.41; 95% CI, 0.30–0.56) and the risk of OAB in the lowest quartile of alcohol consumption quantity was significantly higher than the second (OR 0.58; 95% CI, 0.47–0.70), third (OR 0.49; 95% CI, 0.39–0.62), and highest quartiles groups (OR 0.58; 95% CI, 0.45–0.75). The adjusted model revealed that only patients in the 10+ drinks/month group had a significantly lower risk of OAB than did those in the nondrinker group (OR = 0.64; 95% CI = 0.45–0.92), while the other two groups had similar risks. Furthermore, no significant association was found for the highest quartiles in the adjusted model; however, the second and third quartiles of alcohol consumption quantity group still exhibited obvious associations. These findings suggest that higher alcohol consumption, when appropriate, is associated with a lower risk of OAB compared to nondrinkers and the lowest quartile of alcohol consumption quantity group, even after adjusting for age, sex, race, and comorbidities.ConclusionIn conclusion, our findings revealed a significant association between alcohol consumption and the incidence of OAB in the study population. In terms of long-term effects, alcohol may not be a risk factor for OAB. These factors may represent intervention targets for lowering the risk and severity of OAB symptoms, but this needs to be confirmed in large clinical trials.
Identification of deleterious variants associated with male infertility genes in a cohort of idiopathic hypospermatogenesis patients
Authors: Sharma Nisha , Halder Ashutosh , Kaushal Seema , Kumar Manoj , Jain Manish 
Published: January 2025

IntroductionHypospermatogenesis is a common histopathological subtype of non-obstructive azoospermia and is characterized by a decrease in the total number of germ cells within the seminiferous tubule as a result of spermatogenic failure. Determination of genetic factors before intracytoplasmic sperm injection can prevent the inheritance of these factors, as hypospermatogenesis patients gives high successful sperm retrieval rate. This study aimed to identify the structural variants associated with idiopathic hypospermatogenesis (iHS) by analyzing patient cohorts diagnosed with azoospermia using whole exome sequencing.MethodsIt is a hospital-based observational study in which patients reporting with azoospermia due to spermatogenic failure were recruited prospectively. Comprehensive clinical history, blood samples, semen analysis parameters, and reproductive endocrine evaluation reports of 51 hypospermatogenesis patients were collected. The known genetic causes were investigated using XY fluorescent in situ hybridization and Yq microdeletion for exclusion. Whole exome sequencing was performed, and the data of 42 iHS patients was analyzed to identify single nucleotide variants associated with diagnostically important male infertility genes.ResultsGenomic analysis of SNVs identified rare deleterious candidate variants in CFTR (c.1265C&gt;T; p.Ser422Phe), CYP21A2 (c.955C&gt;T; p.Gln319Glu), SRD5A2 (c.737G&gt;A; p.Arg245Gln), LHCGR (c.378A&gt;C; p.Lys126Asn) and AR (c.2179C&gt;A; p.Arg727Ser) genes associated with 7/42 idiopathic hypospermatogenesis patients. In silico analysis of variants shows deleterious and probably damaging effects on canonical transcripts of the genes.DiscussionThis exploratory genomic analysis conducted on idiopathic hypospermatogenesis patients shows prevalence of rare deleterious candidate variants in genes associated with human male infertility. The candidate variants in idiopathic hypospermatogenesis patients are heterozygous and genotypically associated with syndromic male infertility. The symptomatic heterozygosity leading to mild spermatogenic failure resulting in hypospermatogenesis points towards a multifactorial etiology of the disease. This study justifies the importance of genetic screening of idiopathic hypospermatogenesis patients for the presence of structural variants in known human male infertility genes.
Characterization of the Different Chemical Components and Nutritional Properties of Two Eryngium Species
Authors: Roudbari Mozhgan , Barzegar Mohsen , Sendra Esther , Casanova-Martínez Isabel , Rodríguez-Estrada Marcos , Carbonell-Barrachina Ángel A. 
Published: January 2025

This study aimed to investigate the nutritional value and potential for herbal tea production of two species Eryngium. The analysis includes the quantification of lipids, proteins, organic acids (HPLC-MS), sugars (HPLC-MS), phenolic compounds (HPLC-MS-MS), volatile compounds (GC-MS), fatty acids (GC-MS), amino acids (HPLC-MS-MS), some minerals (ICP-MS), total phenolic content, and antioxidant activities of Eryngium billardieri flowers (EBF) and thorns (EBT), as well as Eryngium planum flowers (EPF) and thorns (EPT). The results indicate that EPF and EPT exhibit elevated levels of protein (11.2%) and sugars (224.2 mg/gdw), respectively. Whereas, EBF demonstrates a higher concentration of amino acids (7.13 mg/100 gdw) and total phenolic content (19.25 mg GAE/gdw), which correlates with pronounced antioxidant properties. Oleic acid was notable in E. billardieri, while linoleic and α-linolenic acids were predominant in E. planum. Furthermore, essential minerals such as Fe, Mn, Zn, Mg, K, Ca, and P were also determined. Sensory evaluations by panelists confirmed that tea derived from the studied species possesses favorable taste and flavor profiles, attributed to its rich volatile compounds. These findings highlight the nutritional value of Eryngium species as a functional ingredient in the food industry. Additionally, their antioxidant properties suggest promising uses in pharmaceutical applications.
Effects of Dazomet Fumigation Combined with Trichoderma harzianum on Soil Microbial Community Structure of Continuously Cropped Strawberry
Authors: Wu Ran , Li Yan , Meng Jian , Han Jiangwei 
Published: January 2025

To study the effects of dazomet, which is a soil fumigant and microbial inoculant (Trichoderma harzianum) on the bacterial and fungal diversity of continuously cropped strawberry soil, this work aimed to provide a theoretical basis for the treatment of continuously cropped strawberry. The soil of a strawberry greenhouse with continuous cropping for 10 years was used as the research object, and rhizosphere soil samples from strawberry continuous cropping were collected before dazomet fumigation (M0), after dazomet fumigation (M1), after dazomet fumigation in the fruit early phase without the addition of T. harzianum (H0), and after the addition of T. harzianum (H1). The diversity of soil bacteria and fungi was detected via the Illumina HiSeq high-throughput sequencing platform. The results revealed that the richness and diversity of the soil bacterial and fungal communities decreased after dazomet fumigation and that the addition of T. harzianum after dazomet fumigation increased both the richness of the soil bacterial communities and the diversity of the fungal communities. At the genus level, the composition ratios of the soil bacterial and fungal communities in the different treatments also substantially differed. The bacterial community compositions of H0 and H1 were the most similar, while the fungal community compositions of M0 and H0 were the most similar. The analysis of beneficial and harmful dominant genera in the soil revealed that dazomet fumigation could kill pathogenic fungi such as Ilyonectria and Fusarium in the soil and reduce beneficial bacteria and fungi such as Streptomyces, Flavobacterium, Mortierella, and Talaromyces. The addition of T. harzianum to dazomet fumigation can increase the abundance of beneficial bacteria and fungi such as Bacillus, Flavobacterium, and Cladorrhinum in the soil as well as reduce the abundance of pathogenic fungi such as Monilinia, Ilyonectria, and Fusarium. In summary, the use of dazomet fumigation combined with microbial inoculation changed the microbial structure of continuously cropped soil, increased the abundance of beneficial bacteria and fungi, and reduced the abundance of the vast majority of pathogenic fungi in the soil. Therefore, dazomet fumigation combined with microbial inoculation is an effective method for overcoming obstacles to continuous cropping of the strawberry.
Comparative Analysis of Five Moroccan Thyme Species: Insights into Chemical Composition, Antioxidant Potential, Anti-Enzymatic Properties, and Insecticidal Effects
Authors: Ouknin Mohamed , Alahyane Hassan , Ait Aabd Naima , Elgadi Sara , Lghazi Youssef , Majidi Lhou 
Published: January 2025

This study aimed to investigate the chemical composition and bioactivities of essential oils (EOs) from five Moroccan thyme species: Thymus broussonetii subsp. broussonetii, T. maroccanus, T. willdenowii, T. zygis subsp. gracilis, and T. satureioides, collected from various geographical regions. Gas chromatography–mass spectrometry (GC-MS) identified thymol, p-cymene, borneol, γ-terpinene, carvacrol, α-pinene, and camphene as major constituents, with variations across species. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) revealed important levels of calcium (450.6–712.2 mg/kg), potassium (255.4–420.7 mg/kg), magnesium (97.3–150.7 mg/kg), and iron (1.95–15.1 mg/kg). The EOs demonstrated strong antioxidant activity in DPPH, FRAP, and β-carotene bleaching assays. Insecticidal activity against Aphis gossypii revealed the highest efficacy with T. willdenowii EO (LC50 = 6.2 µL/mL), followed by T. maroccanus and T. zygis subsp. gracilis. Additionally, the EOs exhibited potent enzyme inhibitory effects at 1 mg/mL on acetylcholinesterase (83.1–96.4%), tyrosinase (77.5–89.6%), and α-glucosidase (79.4–89.5%). These findings suggest that thyme EOs, particularly from T. willdenowii, T. zygis, and T. maroccanus, are promising candidates for integrated pest management and natural enzyme inhibitors. Their potential applications in medicinal and pharmaceutical fields underscore the need for further research to optimize their use under specific conditions and dosages.
Overexpression of AtruLEA1 from Acer truncatum Bunge Enhanced Arabidopsis Drought and Salt Tolerance by Improving ROS-Scavenging Capability
Authors: Li Shaofeng , Meng Huijing , Yang Yanfei , Zhao Jinna , Xia Yongxiu , Wang Shaoli , Wang Fei , Zheng Guangshun , Li Jianbo 
Published: January 2025

Late embryonic developmental abundant (LEA) genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. Acer truncatum Bunge is a promising candidate tree species for investigating the tolerance mechanism of woody plants against abiotic stress. In our previous study, AtruLEA1 was identified as being associated with seed drought tolerance. In this study, LEA1 was cloned from A. truncatum Bunge and functionally characterized. AtruLEA1 encodes an LEA protein and is located in the nucleus. Phylogenetic tree analysis revealed a recent affinity of the AtruLEA1 protein to AT3G15760.1. Overexpression of AtruLEA1 resulted in enhanced tolerance of Arabidopsis thaliana to drought and salt stress and heightened the ABA sensitivity. Compared to wild-type (WT) plants, plants with overexpressed AtruLEA1 exhibited increased activities of antioxidant enzymes under drought stress. Meanwhile, the ROS level of transgenic Arabidopsis was significantly less than that of the WT. Additionally, the stoma density and stoma openness of AtruLEA1 Arabidopsis were higher compared to those in the WT Arabidopsis under salt and drought stress conditions, which ensures that the biomass and relative water content of transgenic Arabidopsis are significantly better than those of the WT. These results indicated that AtruLEA1 was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by abiotic stress. These results indicate a positive role of AtruLEA1 in drought and salt stress and provide theoretical evidence in the direction of cultivating resistant plants.
Genetic Links Between Gastrointestinal Disorders and Kidney Stone Disease
Authors: Wang Yicun , Zhao Zhiyi , Wang Runyi , Hu Xiaopeng 
Published: January 2025

Background:
Epidemiological associations between kidney stone disease (KSD) and gastrointestinal disorders have been reported, and intestinal homeostasis plays a critical role in stone formation. However, the underlying intrinsic link is not adequately understood. This study aims to investigate the genetic associations between these two types of diseases.

Methods
We obtained summary statistics from large-scale genome-wide association studies of KSD and gastrointestinal diseases, including gastroesophageal reflux disease (GERD), peptic ulcer disease, inflammatory bowel disease and its subtypes, irritable bowel syndrome (IBS) and diverticular disease (N = 311,254 to 720,199). Their overall genetic correlations were first estimated. We then detected the shared genetic architecture, including pleiotropic single nucleotide polymorphisms (SNPs), loci, genes and biological processes, through cross-trait analyses. In addition, bidirectional Mendelian randomization (MR) analysis was performed to look for their causal relationships.

Results
We found significantly positive genetic correlations between KSD and all five gastrointestinal diseases. The cross-trait analysis identified 3184 potential pleiotropic SNPs, and 33 of which were pleiotropic loci shared by the two disorders. Gene-level analyses revealed 8 pleiotropic causal genes, primarily enriched in biological pathways involving ion homeostasis and response to vitamin D. In the MR analysis, we detected causal effects from GERD, IBS and Crohn’s disease to KSD, while no reverse causality was observed.

Conclusions
Our study demonstrated the positive genetic links between KSD and gastrointestinal diseases and reported pleiotropic variants, loci, and genes, implicating potential biological mechanisms in the pathogenesis of stone disease. These findings further support the role of the gut-kidney axis and provide a genetic basis for the prevention, coregulation and treatment of these diseases.
La estrategia curricular de educación ambiental en la superación de los promotores del programa educa a tu hijo.
Authors: Fumero Pérez Ariolki , Sánchez Rodríguez Circe , Fumero Pérez Ariolki 
Published: January 2025

La investigación es el resultado de la revisión de la estrategia curricular de educación ambiental en la superación de los promotores del PETH, los que revelan: Insuficiente tratamiento a la educación ambiental, que permita solucionar tareas del objeto de la profesión a través de la superación profesional a los promotores del programa comunitario Educa a tu hijo, con énfasis en la asignatura Didáctica del Conocimiento del Mundo Natural y Social. La necesidad de atención a los aspectos teóricos – metodológicos con respecto a la educación ambiental y su tratamiento durante el proceso docente educativo a partir de la vinculación coherente de las estrategias curriculares y, en particular, la del trabajo con la educación ambiental, para el logro de un aprendizaje desarrollador donde se vinculen los diferentes agentes y agencias educativas de la comunidad. Siendo el objetivo: Elaborar una estrategia pedagógica de educación ambiental para superar a los promotores del programa comunitario Educa a tu hijo.
Proposed Performance Management System (PMS) using performance prism indicators case study at NGO XYZ
Authors: Nabilla Faradhina Astri , Wibisono Dermawan 
Published: January 2025

Purpose: The NGO XYZ, a prominent Indonesian NGO, faces the dual challenge of demonstrating accountability and sustaining growth in the competitive philanthropic sector. This study proposes a Performance Management System (PMS) tailored to NGO XYZ using the Performance Prism framework, which emphasizes stakeholder satisfaction, contributions, strategies, processes, and capabilities.
Method: This research utilizes qualitative and quantitative methods, including organizational analysis, stakeholder interviews, and the Analytic Hierarchy Process (AHP), to identify and prioritize key performance indicators (KPIs).
Results: These indicators span multiple stakeholder groups including donors, beneficiaries, employees, regulators, and suppliers. This study highlights the importance of metrics such as donor growth, program impact, and regulatory compliance in aligning NGO XYZ’s strategic objectives with its operational practices. The key findings reveal that donor satisfaction and retention, beneficiary empowerment, and employee productivity are critical for enhancing the performance of NGO XYZ. The research identifies gaps in innovation, donor engagement, and program sustainability and recommends strategic interventions to address these challenges. This PMS framework aims to optimize resource allocation, improve operational efficiency, and enhance stakeholder engagement. It provides actionable insights for NGOs to measure and improve their social impact, while maintaining accountability and transparency. By adopting these strategies, the NGO XYZ can strengthen its role in addressing societal challenges and promoting sustainable community development.
Pengaruh Online Customer Review dan Flash Sale Promotion yang Dimediasi oleh Sikap terhadap Minat Beli Skincare Skintific pada Marketplace Shopee
Authors: Wulandari Nita , Isa Muzakar 
Published: January 2025

Purpose: the purpose of this study is to examine the impact of online customer reviews and flash sale promotions on purchase interest in skintific skincare products with consumer attitude as a mediating variable
Research methodology: study uses a quantitative approach with closed questionnaire survey techniques. The reseach sample consisted of students from several universities in Surakarta with data analysis carried out using PLS 3.0
Results: The findings reveal that Online Customer Reviews and Flash Sale Promotions have a positive and significant influence on Purchase Interest. Additionally, Consumer Attitude acts as a mediator in the connection between these variables and Purchase Interest
Limitations: The study lie in the use of only two variable independent , closed questionnaire method and limited sample coverage to students in Surakarta.
Contribution: The reseach helps to advance the field of digital marketing strategies, especially in increasing the effectiveness of online promotions and utilizing customer reviews to encourage purchase interest