bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nephrogenic Adenoma: Immunohistochemical Evaluation for Its Etiology and Differentiation From Prostatic Adenocarcinoma
Authors: Xiao Guang-Qian , Burstein David E. , Miller Lorraine K. , Unger Pamela D. 
Published: February 2021

Abstract
Context.—Nephrogenic adenoma is a rare benign lesion of the urinary tract. Owing to its strong association with a history of urinary tract irritation, nephrogenic adenoma was initially thought to originate from urothelial metaplasia; however, no solid proof of this association has been found. More recent investigation has pointed to a renal tubular cause. In addition to its uncertain origin, there can be diagnostic difficulty in distinguishing nephrogenic adenoma from prostatic carcinoma, particularly when dealing with lesions from the prostatic urethra.
Objective.—To elucidate a possible histogenic relationship between nephrogenic adenoma and renal tubules, and also to evaluate the role of immunohistochemistry in the diagnostic distinction between nephrogenic adenoma and prostate carcinoma.
Design.—Immunohistochemical studies were performed for P504S, prostate-specific antigen, CD10, p63, and epithelial membrane antigen on 9 cases of nephrogenic adenoma, 10 cases of normal renal parenchyma, and 10 cases of prostatic tissue, both benign and malignant.
Results.—Nephrogenic adenoma shares the same immunohistochemical profile as distal renal tubules: both are positive for P504S and epithelial membrane antigen and negative for p63, CD10, and prostate-specific antigen. Prostatic adenocarcinoma tissue was positive for P504S and prostate-specific antigen, and normal prostatic gland tissue was positive for prostate-specific antigen and negative for P504S; p63-stained basal cells in normal prostatic gland tissue but did not react with prostatic adenocarcinoma tissue. The CD10 inconsistently stained normal and neoplastic prostatic gland tissue. Epithelial membrane antigen stain was negative in prostatic carcinoma, with rare occasional reactivity in normal prostatic glands.
Conclusion.—These findings provide supporting evidence that nephrogenic adenoma is derived from distal renal tubules. Our results also demonstrated that the combination of P504S and prostate-specific antigen with epithelial membrane antigen is a valuable tool in distinguishing prostatic carcinoma from nephrogenic adenoma.
Dual-Track Pathway of Bladder Carcinogenesis: Practical Implications
Authors: Spiess Philippe E. , Czerniak Bogdan 
Published: February 2021

Abstract
Context.—The concept of a dual-track pathway in bladder carcinogenesis postulates that bladder cancer develops via 2 distinct but somewhat overlapping pathways, termed the papillary and nonpapillary. Approximately 80% of bladder carcinomas consist of superficial exophytic papillary lesions that originate from urothelial hyperplasia. These typically low-grade papillary tumors may recur, but they rarely invade the bladder wall or metastasize. The remaining 15% to 20% of tumors represent high-grade solid nonpapillary bladder carcinoma, which arise from high-grade intraurothelial neoplasia. These tumors aggressively invade the bladder wall and have a high propensity for distant metastasis.
Objective.—To summarize the scientific literature and provide a clinicopathologic review of the dual-track concept of bladder carcinogenesis with its important implications.
Data Sources.—Relevant articles indexed in PubMed (National Library of Medicine) between 1974 and 2005.
Conclusions.—Although the characteristics of papillary and nonpapillary tumors are quite dissimilar, current evidence implies that both forms of bladder cancer start as a clinically occult clonal expansion of preneoplastic lesions that occupy large areas of the bladder mucosa.
Dual Surface Immunoglobulin Light-Chain Expression in B-Cell Lymphoproliferative Disorders
Authors: Xu Dongsheng 
Published: February 2021

AbstractContext.—Mature B lymphocytes exhibit allelic exclusion in which only a single class of immunoglobulin heavy chain and a single class of light chain, either κ or λ, are expressed. There have been several reports indicating that double light-chain gene rearrangements or dual light-chain expression can occur in B-cell malignant neoplasms, including chronic lymphocytic leukemia. Currently, it is a common notion that demonstration of light-chain restriction in a B-cell population is generally considered proof of monoclonality and indicates malignancy.Objective.—To increase awareness of the existence of the dual κ/λ immunoglobulin light-chain expressing B-cell leukemia/lymphoma, to emphasize the importance of visual inspection of flow cytometric data, and to present the guidelines for flow cytometric interpretation of dual κ/λ coexpressing populations.Data Sources.—Through comprehensive literature review, this article discusses the current understandings regarding the dual light-chain expression in B-cell neoplasms, the cellular and molecular mechanisms, and the clinical and diagnostic implications of dual light-chain expression.Conclusions.—Dual κ/λ light-chain expressing B-cell leukemia/lymphomas do exist. Recognition of the dual κ/ λ light-chain expression on B cells has diagnostic implication in leukemia/lymphoma immunophenotyping.
Frozen Section Discrepancy in the Evaluation of Central Nervous System Tumors
Authors: Plesec Thomas P. , Prayson Richard A. 
Published: February 2021

Abstract
Context.—Frozen section (FS) evaluation of central nervous system (CNS) lesions provides an assessment of specimen adequacy and facilitates triage for ancillary studies. Frozen section also provides an accurate preliminary diagnosis; however, certain lesions are recognized to cause diagnostic challenges at FS.
Objective.—To identify cases in which there was a discrepancy between the FS diagnosis and final diagnosis in the case to heighten awareness of common diagnostic pitfalls in surgical neuropathology.
Design.—All CNS FS cases involving a tumor diagnosis at FS or permanent section (N = 2156) from September 1997 until June 2005 were retrospectively reviewed. Discrepancies between the FS and final diagnoses were identified.
Results.—Of the 2156 cases identified, 57 (2.7%) discrepant diagnoses were found. Twelve (21.1%) of 57 discrepancies involved errors in classification of spindle cell lesions, most commonly confusing schwannomas or meningiomas with other lesions. Twelve (21.1%) of 57 cases involved errors in differentiating oligodendrogliomas from astrocytomas. Nine (15.8%) of 57 discrepancies involved errors in the diagnosis of CNS lymphoma. Eight (14.0%) of 57 cases involved errors in differentiating reactive from neoplastic processes, most frequently gliosis versus glioma. Four (7.0%) of 57 discrepancies involved errors in the overgrading of tumors. The remaining 12 (21.1%) of 57 cases included an assortment of other discrepancies.
Conclusions.—Frozen section of CNS neoplastic processes can be highly accurate. Less than 3% of FS diagnoses in 1 institution's experience were discrepant with the final diagnoses. Approximately 80% of the discrepant cases were classified into 5 categories: spindle cell lesions, astrocytoma versus oligodendroglioma, differential diagnosis of CNS lymphoma, reactive versus neoplastic process, and tumor overgrading. Awareness of these pitfalls may help in further increasing diagnostic accuracy.
α-Methylacyl CoA Racemase in Pulmonary Adenocarcinoma, Squamous Cell Carcinoma, and Neuroendocrine Tumors: Expression and Survival Analysis
Authors: Shilo Konstantin , Dracheva Tatiana , Mani Haresh , Fukuoka Junya , Sesterhenn Isabell A. , Chu Wei-Sing , Shih Joanna H. , Jen Jin , Travis William D. , Franks Teri J. 
Published: February 2021

Abstract
Context.—α-Methylacyl CoA racemase (AMACR) is an oxidative enzyme involved in isomeric transformation of fatty acids entering the beta-oxidation pathway. AMACR serves as a useful marker in establishing a diagnosis of prostatic malignancy; however, limited information is available in regard to its presence in pulmonary neoplasms.
Objective.—To investigate AMACR expression within a spectrum of lung carcinomas and its correlation with patients' survival.
Design.—Four hundred seventy-seven pulmonary carcinomas, including 150 squamous cell carcinomas, 150 adenocarcinomas, 46 typical carcinoids, 31 atypical carcinoids, 28 large cell neuroendocrine carcinomas, and 72 small cell carcinomas, were studied immunohistochemically using tissue microarray–based samples.
Results.—Overall, pulmonary tumors were positive for AMACR in a significant percentage (47%) of cases. Among tumor types, 22% of squamous cell carcinoma, 56% of adenocarcinoma, 72% of typical carcinoid, 52% of atypical carcinoid, 70% of large cell neuroendocrine carcinoma, and 51% of small cell lung carcinoma were positive for AMACR. Furthermore, the Kaplan-Meier analysis revealed that the patients with AMACR-positive small cell carcinoma had better survival (19% vs 5% after 5 years, P = .04) than patients with AMACR-negative tumors. Such survival advantage was seen for patients with stage I–II (P = .01) but not stage III–IV small cell carcinomas (P = .58).
Conclusions.—These results indicate that, similar to prostate cancer, the overexpression of AMACR frequently occurs in pulmonary carcinomas. Additionally, its positive correlation with outcome of stage I–II small cell lung carcinoma warrants further investigation of the AMACR role in the prognosis of this tumor.
Improved Filter Method for Urine Sediment Detection of Urothelial Carcinoma by Fluorescence In Situ Hybridization
Authors: Meiers Isabelle , Singh Harpreet , Hossain Deloar , Lang Kevin , Liu Lina , Qian Junqi , Verhest Alain P. , Bostwick David G. 
Published: February 2021

AbstractContext.—Fluorescence in situ hybridization (FISH) of voided urine sediment is a sensitive and specific test for the detection of urothelial carcinoma. The time required for slide preparation using the conventional cytospin method is lengthy.Objective.—To present an alternative to the conventional cytospin method.Design.—We compared the results of an improved filter monolayer method with published results of the conventional cytospin method. A total of 624 patients with cytology and FISH analyses were followed with cystoscopy and/ or bladder biopsy. Fluorescence in situ hybridization analysis was performed on 624 cases using fluorescence-labeled probes to the pericentromeric regions of chromosomes 3, 7, and 17 and band 9p21; cytology was also performed in all cases.Results.—A total of 217 (34.7%) of 624 patients had follow-up bladder biopsies, and 170 of these (78.3%) had urothelial carcinoma. The sensitivity for cancer detection was higher for FISH than for urine cytology (92.9% [158/ 170] for FISH vs 72.9% [124/170] for urine cytology, P = &lt;5%). The specificity was equivalent for FISH and urine cytology (97.5% [443/454] for FISH vs 92.2% [419/454] for cytology). The sensitivity for FISH was better (92.9% vs 81%), and there was no significant difference in specificity (97.5% vs 96%) between the filter method and the conventional cytospin method. Unlike the conventional cytospin method, the filter method did not require multiple centrifugation and decantation steps or investment in dedicated equipment.Conclusions.—The improved filter method was faster, easier, and less expensive than published results with the conventional cytospin method with better sensitivity and equivalent specificity.
Omentectomy for Gynecologic Cancer: How Much Sampling Is Adequate for Microscopic Examination?
Authors: Usubütün Alp , Ozseker Havva Solak , Himmetoglu Cigdem , Balcı Serdar , Ayhan Ali 
Published: February 2021

Abstract
Context.—Detecting omental metastasis is crucial for staging and treatment of endometrial and ovarian carcinoma.
Objective.—To determine the optimal omental sampling for omentectomies to ascertain the stage of the disease in a cost-effective way.
Design.—We reevaluated 258 omentectomies that were performed due to ovarian or endometrial carcinoma. A total of 116 cases were retrospectively studied, and 142 cases were prospectively studied. For prospective study, 10 to 16 blocks were sampled if the omentum showed no signs of gross tumor. Mean omental block sample frequency of 2 groups with the negative macroscopy but with or without microscopic tumor have been compared using an independent samples t test.
Results.—Seven patients had no evidence of tumor metastasis on gross examination but had microscopic tumor metastasis. The mean numbers of blocks were 6.4 for patients having microscopic tumor without macroscopic involvement and 7.8 for patients having neither microscopic nor macroscopic involvement. Approximately twice as many samples were taken in the prospective analysis when compared with retrospective analysis. Two cases with microscopic omental metastasis that had no macroscopic involvement at first impression were reevaluated retrospectively and found to contain 0.3- to 0.5-cm white nodules. The rate of omental metastasis increased with the grade of the tumor (P = .005).
Conclusion.—Careful macroscopic examination is the most important step in detecting small omental metastasis. For cases with gross tumor, one section is sufficient. If a macroscopic lesion is not detectable and the patient has a high-grade tumor that will necessitate an adjuvant therapy, 3 to 5 samples seem sufficient for staging. Further studies are needed to determine the optimum sample size for tumors having a low risk of metastasis.
Utility of Tissue-Specific Transcription Factors Thyroid Transcription Factor 1 and Cdx2 in Determining the Primary Site of Metastatic Adenocarcinomas to the Brain
Authors: Strickland-Marmol Leah B. , Khoor Andras , Livingston Sandra K. , Rojiani Amyn 
Published: February 2021

AbstractContext.—Brain metastases of adenocarcinoma of unknown primary pose a diagnostic dilemma to the surgical pathologist. Although the most common source in these cases is the lung, determining a primary source is difficult on routinely stained slides. Immunohistochemical stain panels including differential cytokeratins, hormone receptors, and breast-specific proteins are commonly used in these cases. Recently, attention has turned to tissue-specific transcription factors, such as thyroid transcription factor 1 (TTF-1) and Cdx2, in the appraisal of metastatic adenocarcinomas.Objective.—To characterize the previously unpublished immunohistochemical expression of the relatively new tissue-specific transcription factor Cdx2 in metastatic adenocarcinomas to the brain.Design.—We reviewed the surgical pathology files of the H. Lee Moffitt Cancer Center and Research Institute, Tampa, Fla, and retrieved 38 consecutive cases of metastatic adenocarcinoma (22 pulmonary, 10 breast, 6 gastrointestinal [2 esophagus/gastroesophageal junction, 4 colorectal]) to the brain with confirmation of the primary site by chart review and histologic evaluation. Sections were immunohistochemically stained with antibodies to TTF-1, Cdx2, and cytokeratins 7 and 20 by standard methods.Results.—Specificities and positive predictive values for Cdx2 and TTF-1 equaled 100% for metastatic gastrointestinal and pulmonary adenocarcinomas, respectively. The negative predictive value of Cdx2 was also very high at 97%.Conclusions.—Cdx2 is a specific and valuable tool for the surgical pathologist when faced with the common problem of metastatic adenocarcinoma of unknown primary. In conjunction with TTF-1, cytokeratin 7, and cytokeratin 20, Cdx2 can accurately differentiate the most common sources of metastatic adenocarcinoma to the brain.
Sensitivity of Peripheral Blood Smear Review for the Diagnosis of Candida Fungemia
Authors: Branda John A. , Ferraro Mary Jane , Kratz Alexander 
Published: February 2021

Abstract
Context.—Case reports have described detection of candidemia by examination of peripheral blood smears. It is unclear whether this method has wider applicability for early detection of fungemia.
Objective.—To determine the sensitivity of smear review for detecting candidemia.
Design.—Normal and cytopenic blood was spiked with increasing concentrations of yeast. Smears were prepared and reviewed by a pathologist and by technical staff. Staff members blinded to the purpose of the study first performed a routine slide review and then a targeted review for yeast.
Results.—The pathologist detected isolated yeast forms at a concentration of 1 to 5 × 105 colony-forming units (CFU)/mL. When blinded to the purpose of the study, technical staff could detect Candida in most samples when the yeast concentration was 1 to 5 × 107 CFU/mL, but found it in only a small fraction of samples with lower concentrations. When asked to examine the smears specifically for yeast, they could detect it in most samples containing 1 to 5 × 106 CFU/mL.
Conclusions.—Detection of candidemia by peripheral blood smear examination requires a yeast concentration of 1 to 5 × 105 CFU/mL or greater. This degree of fungemia is unusual; therefore, detection of candidemia by blood smear review will not be possible in most cases. Sensitivity of smear review for yeast detection is greatly increased if the microscopist is specifically directed to look for the presence of yeast.
Endosalpingiosis Is Negative for GATA3
Authors: White Marissa J. , Vang Russell , Argani Pedram , Cimino-Mathews Ashley 
Published: February 2021

Context.—
Endosalpingiosis is a benign Müllerian inclusion that can mimic metastatic low-grade mammary carcinoma, particularly when encountered in axillary lymph nodes excised for breast cancer staging. Immunohistochemistry can be useful in histologically ambiguous cases, and a targeted immunopanel should include a marker of gynecologic tract origin and a marker of mammary origin. GATA3 is a sensitive immunomarker for breast carcinoma, but the immunoreactivity of GATA3 in endosalpingiosis has not been systematically evaluated.

Objective.—
To evaluate whether GATA3 immunohistochemistry could be used to differentiate endosalpingiosis from metastatic mammary carcinoma.

Design.—
Whole slide sections of 15 cases of endosalpingiosis involving nonneoplastic tissues were subjected to GATA3 immunohistochemistry. Nuclear GATA3 labeling was scored as percentage and intensity labeling, with any labeling considered positive; GATA3 labeling was recorded in all cells present in the sections.

Results.—
Half (47%, n = 7 of 15) of the endosalpingiosis cases involved lymph nodes (2 axillary, 5 pelvic) and half (53%, n = 8 of 15) involved pelvic organs or soft tissue (3 myometrial, 2 paratubal, 2 periadnexal soft tissue, and 1 pelvic sidewall). GATA3 immunohistochemistry was negative in all cases of endosalpingiosis, with intact, positive control labeling in lymphocytes. The benign fallopian tube epithelium present on the sections of paratubal endosalpingiosis displayed focal (&lt;5%), weak labeling for GATA3, specifically within the ciliated and secretory cells.

Conclusions.—
These findings support the diagnostic utility of GATA3 immunohistochemistry and its use in a targeted immunopanel to resolve the differential diagnosis of metastatic low-grade mammary carcinoma (GATA3+) and nodal endosalpingiosis (GATA3−).
ELECTROCHEMICAL OBTAINING AND MORPHOLOGY OF ALLOYS’ NANOCOVERINGS IN SYSTEM Re-Сu-Se
Authors: Salakhova Elza A. , Tagiyev Dilgam B. , Kalantarova Parvana E. , Ibrahimova Kamala F. , Ramazanov Mamedali A. , Agamaliyev Zohrab A. 
Published: February 2021

Electrochemical obtaining of nano-coatings in the system Re-Cu-Se on platinum electrode during voltammetric cycling was studied. The research was carried out using sulphate solution containing selenium dioxide, potassium perrenate and copper chloride. For obtaining nano coatings in the Re-Cu-Se system, we used an electrolyte of the following composition (mol/l): 6.9 ∙ 10-4 – 6.9 ∙ 10-3 KReO4 + 9 ∙ 10-4 – 1.8 ∙ 10-2 SeO2 + 6 ∙ 10-4 – 1.2 ∙ 10-2 CuCl2 ∙ 2H2O +2 H2SO4, t = 80 ºC; V = 0.005VS-1; pH = 0.1, electrode - Pt. Based on the studying of the volt-ampere dependences during the joint electroreduction of rhenium (VII), selenium (IV) and copper (II) ions from sulfate electrolytes on a PT electrode, the conditions for the deposition of alloys nanocoatings in the Re-Cu-Se system were established. To study the morphology of films on platinum and copper substrates, the electrode surface was studied using a JEOL JSM7600F scanning electron microscope at various magnifications, and was accordingly subjected to elemental analysis using an Oxford X-MAX 50 detector. The sample has been scanned in the mode of secondary electrons at an accelerating voltage ~ 15 keV. It has been found that agglomerates are observed on the electrode surface, mainly consisting of sphere-shaped particles with an average size of ~ 20-25 nm. The spectrum of characteristic X-ray radiation indicates the presence of these components of the system, allows quantitative analysis of samples. Based on the presented distribution diagram by weight percent, the content of system components is represented by the following ratio: Re-12%, Cu-5%, Se-10%. The presence of characteristic peaks of carbon and oxygen in the spectrum is explained by residual phenomena during obtaining.
Urban Interiority: Emerging Cultural and Spatial Practices
Authors: Atmodiwirjo Paramita , Yatmo Yandi Andri 
Published: February 2021

Discourses on the urban interior recently have emerged as a series of provocations and experimentations that highlight the critical understanding of the urban realm from the interiority perspective. In the fast-moving development of modern global cities, the urban interior concept becomes increasingly important. Cities are fast becoming containers for contemporary spatial practice, with urban spaces becoming melting pots of diverse cultures and communities. Viewing urban settings from the interiority perspective allows us to comprehend unique local characters in particular contexts. This issue of Interiority presents a collection of works that illustrate the expanded understanding of the urban interior, especially in relation to cultural and spatial practice in urban contexts. This issue presents multiple perspectives on understanding the urban interior, raising arguments on how its spatial condition could perform as a container of cultural practice, while simultaneously offering possibilities on manoeuvring within the urban interior context through various ways of reading, interpretation and intervention. These perspectives and approaches promise further possibilities to expand our interior architectural practice in responding not only to current contemporary practice, but also to the future of urban inhabitation.