bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral contraceptives: knowledge and counselling practices of Lebanese community pharmacists
Authors: Chahine Bahia , Souheil Farah Al 
Published: September 2021

Abstract

Objectives
To examine the extent of Lebanese pharmacists’ knowledge about oral contraceptives (OCs), the characteristics influencing their knowledge and the factors that may predict their counselling practices.

Methods
A cross-sectional descriptive study was conducted between January and June 2020 in which a random sample of community pharmacists in Lebanon was asked to complete a self-administered questionnaire. The survey was divided into three sections including demographic information, pharmacists’ knowledge and counselling practices, and their perceptions about OCs.

Key findings
Of the 300 pharmacists invited to participate, 240 (80%) responded. The majority of the pharmacists (255; 85%) had satisfactory general knowledge about the indications, side effects, contraindications and drug interactions of OCs. However, only 173 (57.5%) participants had satisfactory knowledge about the proper use of OCs. Older pharmacists and those who have been in practice for more than 10 years achieved lower knowledge scores; however, those with postgraduate degrees had significantly higher scores. Pharmacists with a postgraduate degree and male pharmacists were less likely to counsel patients compared with their counterparts. Conversely, pharmacists with postgraduate OC training and those who had a private area for consultation presented better counselling practices.

Conclusions
More than half of the participating pharmacists appeared to have satisfactory knowledge about OCs and their proper use; however, limited counselling and key misconceptions were observed in this study.
Challenges for Programmatic Implementation of Oral Cholera Vaccine in India
Authors: Chakraborty Debjit , Kanungo Suman , Kumar Nandy Ranjan , Kumar Deb Alok , Kumar Mukhopadhyay Asish , Dutta Shanta 
Published: September 2021

Abstract
Cholera remains a major contributor of diarrheal diseases and leads to substantial morbidity and mortality, particularly in low socioeconomic settings. Nonavailability of a national cholera control plan in India, compounded by underreporting of cholera cases and deficient accurate cholera hotspot estimates, has made cholera control a challenge. Obstacles in the programmatic introduction of oral cholera vaccine (OCV) lie within the infrastructure—stockpile, costing, distribution system, cold-chain mechanism, vaccine logistics, and lack of strengthened surveillance systems for adverse events following immunization. Sustained political commitment along with collaboration of people working in the media will also determine the policy outcome of OCV introduction in India.
Impairment of Invasion and Maturation and Decreased Selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol Variants
Authors: Palasuwan Duangdao , Palasuwan Attakorn , Boonpeng Kanyarat , Ketprasit Nutpakal , Imwong Mallika , Kulkeaw Kasem 
Published: September 2021

Abstract

Background
Protection against Plasmodium falciparum is observed in a population deficient in glucose-6-phosphate dehydrogenase (G6PD), particularly in African and Mediterranean regions. However, such protection remains unknown among G6PD-deficient individuals in Southeast Asia.

Methods
In this study, we assessed the invasion and maturation of P falciparum K1 in a culture of erythrocytes isolated from Thai subjects carrying Viangchan (871G &gt; A) and Mahidol (487G &gt; A).

Results
We found that the parasites lost their ability to invade hemizygous and homozygous G6PD-deficient erythrocytes of Viangchan and Mahidol variants in the second and third cycles of intraerythrocytic development. It is interesting to note that P falciparum parasites selectively grew in erythrocytes from hemi- and homozygous genotypes with normal G6PD activity. Moreover, externalization of phosphatidylserine upon P falciparum infection was significantly increased only in Viangchan hemizygous variant cells.

Conclusions
This study is the first to show that blockage of invasion in long-term culture and potentially enhanced removal of parasitized erythrocytes were observed for the first time in erythrocytes from Viangchan and Mahidol G6PD-deficient individuals.
Coproduction in medical education during the COVID-19 pandemic: critical components of successful curricular reform
Authors: Gregoire Bruce , Trager Lena , Blum Jessamina 
Published: September 2021

Abstract
The current coronavirus disease 2019 pandemic has greatly affected medical education in unanticipated ways. By introducing the concept of educational coproduction at our institution, we have maintained a flexible and productive curricular environment for all students. The notion of coproduction acknowledges that education is a service that requires recognition of the expertise that both the teacher and the learner bring to the table, in the context of their community and society writ large. Using the coproduction framework allowed for increased communication and improved partnerships among students, educators, clinicians and our community as well as adaptations to a rapidly changing educational environment. Embracing the idea of coproduction is a valuable concept for institutions to consider during this time and in the future post-pandemic period.
How safe is prehospital care? A systematic review
Authors: O’connor Paul , O’malley Roisin , Lambe Kathryn , Byrne Dara , Lydon SinÉad 
Published: September 2021

Abstract

Background
As compared to other domains of healthcare, little is known about patient safety incidents (PSIs) in prehospital care. The aims of our systematic review were to identify how the prevalence and level of harm associated with PSIs in prehospital care are assessed; the frequency of PSIs in prehospital care; and the harm associated with PSIs in prehospital care.

Method
Searches were conducted of Medline, Web of Science, PsycInfo, CINAHL, Academic Search Complete and the grey literature. Reference lists of included studies and existing related reviews were also screened. English-language, peer-reviewed studies reporting data on number/frequency of PSIs and/or harm associated with PSIs were included. Two researchers independently extracted data from the studies and carried out a critical appraisal using the Quality Assessment Tool for Studies with Diverse Designs (QATSDD).

Results
Of the 22 included papers, 16 (73%) used data from record reviews, and 6 (27%) from incident reports. The frequency of PSIs in prehospital care was found to be a median of 5.9 per 100 records/transports/patients. A higher prevalence of PSIs was identified within studies that used record review data (9.9 per 100 records/transports/patients) as compared to incident reports (0.3 per records/transports/patients). Across the studies that reported harm, a median of 15.6% of PSIs were found to result in harm. Studies that utilized record review data reported that a median of 6.5% of the PSIs resulted in harm. For data from incident reporting systems, a median of 54.6% of incidents were associated with harm. The mean QATSDD score was 25.6 (SD = 4.1, range = 16–34).

Conclusions
This systematic review gives direction as to how to advance methods for identifying PSIs in prehospital care and assessing the extent to which patients are harmed.
Properties of an acid-tolerant, persistent Cheddar cheese isolate, Lacticaseibacillus paracasei GCRL163
Authors: Shah Syed S , Al-Naseri Ali , Rouch Duncan , Bowman John P , Wilson Richard , Baker Anthony L , Britz Margaret L 
Published: September 2021

Abstract
The distinctive flavours in hard cheeses are attributed largely to the activity of nonstarter lactic acid bacteria (NSLAB) which dominate the cheese matrix during maturation after lactose is consumed. Understanding how different strains of NSLAB survive, compete, and scavenge available nutrients is fundamental to selecting strains as potential adjunct starters which may influence product traits. Three Lacticaseibacillus paracasei isolates which dominated at different stages over 63-week maturation periods of Australian Cheddar cheeses had the same molecular biotype. They shared many phenotypic traits, including salt tolerance, optimum growth temperature, growth on N-acetylglucosamine and N-acetylgalactosamine plus delayed growth on D-ribose, carbon sources likely present in cheese due to bacterial autolysis. However, strains 124 and 163 (later named GCRL163) survived longer at low pH and grew on D-tagatose and D-mannitol, differentiating this phenotype from strain 122. When cultured on growth-limiting lactose (0.2%, wt/vol) in the presence of high concentrations of L-leucine and other amino acids, GCRL163 produced, and subsequently consumed lactate, forming acetic and formic acids, and demonstrated temporal accumulation of intermediates in pyruvate metabolism in long-term cultures. Strain GCRL163 grew in Tween 80-tryptone broths, a trait not shared by all L. casei-group dairy isolates screened in this study. Including citrate in this medium stimulated growth of GCRL163 above citrate alone, suggesting cometabolism of citrate and Tween 80. Proteomic analysis of cytosolic proteins indicated that growth in Tween 80 produced a higher stress state and increased relative abundance of three cell envelope proteinases (CEPs) (including PrtP and Dumpy), amongst over 230 differentially expressed proteins.
Cost-effectiveness analysis using lifetime attributable risk of proton beam therapy for pediatric medulloblastoma in Japan
Authors: Yoshimura Takaaki , Tamori Honoka , Morii Yasuhiro , Hashimoto Takayuki , Shimizu Shinichi , Ogasawara Katsuhiko 
Published: September 2021

Abstract
Compared to conventional X-ray therapy, proton beam therapy (PBT) has more clinical and physical advantages such as irradiation dose reduction to normal tissues for pediatric medulloblastoma. However, PBT is expensive. We aimed to compare the cost-effectiveness of PBT for pediatric medulloblastoma with that of conventional X-ray therapy, while focusing on radiation-induced secondary cancers, which are rare, serious and negatively affect a patient’s quality of life (QOL). Based on a systematic review, a decision tree model was used for the cost-effectiveness analysis. This analysis was performed from the perspective of health care payers; the cost was estimated from medical fees. The target population was pediatric patients with medulloblastoma below 14 years old. The time horizon was set at 7.7 years after medulloblastoma treatment. The primary outcome was the incremental cost-effectiveness ratio (ICER), which was defined as the ratio of the difference in cost and lifetime attributable risk (LAR) between conventional X-ray therapy and PBT. The discount rate was set at 2% annually. Sensitivity analyses were performed to model uncertainty. Cost and LAR in conventional X-ray therapy and PBT were Japanese yen (JPY) 1 067 608 and JPY 2436061 and 42% and 7%, respectively. The ICER was JPY 3856398/LAR. In conclusion, PBT is more cost-effective than conventional X-ray therapy in reducing the risk of radiation-induced secondary cancers in pediatric medulloblastoma. Thus, our constructed ICER using LAR is one of the valid indicators for cost-effectiveness analysis in radiation-induced secondary cancer.
Petrous apex lesion following meningitis: management and discussion
Authors: Elmaraghy Charles , Bishop Ryan , Barron Christine , Adunka Oliver 
Published: September 2021

Abstract
A 9-year-old male with history of mixed hearing loss presented with petrous apex lesion following episode of meningitis. Serial imaging revealed persistence of the lesion necessitating biopsy to rule out malignancy. Biopsy revealed inflammatory changes. The management of petrous apex lesions following meningitis can be conservative but repeat imaging is necessary to rule out progression and to rule out neoplastic process.
Hepatocellular carcinoma with extrahepatic blood supply from right renal artery
Authors: Sohail Amir Humza , Musa Ahmad , Khan Muhammad Salman , Hashmi Hassan Raza , Salam Basit , Brathwaite Collin E M 
Published: September 2021

Abstract
Extrahepatic blood supply is seen in around 17–27% of hepatocellular carcinoma lesions. Evidence suggests that this extrahepatic supply most commonly originates from a right intercostal artery (70–83%) followed by left intercostal, omental and right renal arteries. Thus a comprehensive knowledge of variations in standard vascular anatomy and cognisance of factors influencing or predicting extrahepatic blood supply in HCC is instrumental in ensuring the success of surgical and interventional procedures. We present the unusual case of a 66-year-old male with HCC in Segment I of the liver with aberrant blood supply from the right renal artery in the absence of any risk factors for extrahepatic circulation. He successfully underwent transarterial chemoembolization. There was no evidence of residual disease on repeat imaging.
Dissecting the metabolic reprogramming of maize root under nitrogen-deficient stress conditions
Authors: Chowdhury Niaz Bahar , Schroeder Wheaton L , Sarkar Debolina , Amiour Nardjis , Quilleré Isabelle , Hirel Bertrand , Maranas Costas D , Saha Rajib 
Published: September 2021

Abstract
The growth and development of maize (Zea mays L.) largely depends on its nutrient uptake through the root. Hence, studying its growth, response, and associated metabolic reprogramming to stress conditions is becoming an important research direction. A genome-scale metabolic model (GSM) for the maize root was developed to study its metabolic reprogramming under nitrogen stress conditions. The model was reconstructed based on the available information from KEGG, UniProt, and MaizeCyc. Transcriptomics data derived from the roots of hydroponically grown maize plants were used to incorporate regulatory constraints in the model and simulate nitrogen-non-limiting (N+) and nitrogen-deficient (N−) condition. Model-predicted flux-sum variability analysis achieved 70% accuracy compared with the experimental change of metabolite levels. In addition to predicting important metabolic reprogramming in central carbon, fatty acid, amino acid, and other secondary metabolism, maize root GSM predicted several metabolites (l-methionine, l-asparagine, l-lysine, cholesterol, and l-pipecolate) playing a regulatory role in the root biomass growth. Furthermore, this study revealed eight phosphatidylcholine and phosphatidylglycerol metabolites which, even though not coupled with biomass production, played a key role in the increased biomass production under N-deficient conditions. Overall, the omics-integrated GSM provides a promising tool to facilitate stress condition analysis for maize root and engineer better stress-tolerant maize genotypes.
Impact of massive binary star and cosmic evolution on gravitational wave observations I: black hole–neutron star mergers
Authors: Broekgaarden Floor S , Berger Edo , Neijssel Coenraad J , Vigna-Gómez Alejandro , Chattopadhyay Debatri , Stevenson Simon , Chruslinska Martyna , Justham Stephen , de Mink Selma E , Mandel Ilya 
Published: September 2021

ABSTRACT
Mergers of black hole–neutron star (BHNS) binaries have now been observed by gravitational wave (GW) detectors with the recent announcement of GW200105 and GW200115. Such observations not only provide confirmation that these systems exist but will also give unique insights into the death of massive stars, the evolution of binary systems and their possible association with gamma-ray bursts, r-process enrichment, and kilonovae. Here, we perform binary population synthesis of isolated BHNS systems in order to present their merger rate and characteristics for ground-based GW observatories. We present the results for 420 different model permutations that explore key uncertainties in our assumptions about massive binary star evolution (e.g. mass transfer, common-envelope evolution, supernovae), and the metallicity-specific star formation rate density, and characterize their relative impacts on our predictions. We find intrinsic local BHNS merger rates spanning $\mathcal {R}_{\rm {m}}^0 \approx$ 4–830 $\, \rm {Gpc}^{-3}$$\, \rm {yr}^{-1}$ for our full range of assumptions. This encompasses the rate inferred from recent BHNS GW detections and would yield detection rates of $\mathcal {R}_{\rm {det}} \approx 1$–180$\, \rm {yr}^{-1}$ for a GW network consisting of LIGO, Virgo, and KAGRA at design sensitivity. We find that the binary evolution and metallicity-specific star formation rate density each impacts the predicted merger rates by order $\mathcal {O}(10)$. We also present predictions for the GW-detected BHNS merger properties and find that all 420 model variations predict that $\lesssim 5{{\ \rm per\ cent}}$ of the BHNS mergers have BH masses $m_{\rm {BH}} \gtrsim 18\, \rm {M}_{\odot }$, total masses $m_{\rm {tot}} \gtrsim 20\, \rm {M}_{\odot }$, chirp masses ${\mathcal {M}}_{\rm {c}} \gtrsim 5.5\, \rm {M}_{\odot }$, and mass ratios qf ≳ 12 or qf ≲ 2. Moreover, we find that massive NSs with $m_{\rm {NS}} \gt 2\, \rm {M}_{\odot }$ are expected to be commonly detected in BHNS mergers in almost all our model variations. Finally, a wide range of $\sim 0{{\ \rm per\ cent}}$ to $70{{\ \rm per\ cent}}$ of the BHNS mergers are predicted to eject mass during the merger. Our results highlight the importance of considering variations in binary evolution and cosmological models when predicting, and eventually evaluating, populations of BHNS mergers.
Gravitational wave observatories may be able to detect hyperbolic encounters of black holes
Authors: Mukherjee Sajal , Mitra Sanjit , Chatterjee Sourav 
Published: September 2021

ABSTRACT
Gravitational wave (GW) astronomy promises to observe different kinds of astrophysical sources. Here, we explore the possibility of detection of GWs from hyperbolic interactions of compact stars with ground-based interferometric detectors. It is believed that a bound compact cluster, such as a globular cluster, can be a primary environment for these interactions. We estimate the detection rates for such events by considering local geometry within the cluster, accounting for scattering probability of compact stars at finite distances, and assuming realistic cluster properties guided by available numerical models, their formation times, and evolution of stars inside them. We find that, even in the conservative limit, it may be possible to detect such black hole encounters in the next few years by the present network of observatories with the ongoing sensitivity upgrades and one to few events per year with the next-generation observatories. In practice, actual detection rates can significantly surpass the estimated average rates, since the chances of finding outliers in a very large population can be high. Such observations (or, no observation) may provide crucial constraints to estimate the number of isolated compact stars in the universe. These detections will be exciting discoveries on their own and will be complementary to observations of binary mergers, bringing us one step closer to address a fundamental question: How many black holes are there in the observable universe?
Formation and persistence of polyglutamine aggregates in mistranslating cells
Authors: Lant Jeremy T , Kiri Rashmi , Duennwald Martin L , O’Donoghue Patrick 
Published: September 2021

AbstractIn neurodegenerative diseases, including pathologies with well-known causative alleles, genetic factors that modify severity or age of onset are not entirely understood. We recently documented the unexpected prevalence of transfer RNA (tRNA) mutants in the human population, including variants that cause amino acid mis-incorporation. We hypothesized that a mistranslating tRNA will exacerbate toxicity and modify the molecular pathology of Huntington's disease-causing alleles. We characterized a tRNAPro mutant that mistranslates proline codons with alanine, and tRNASer mutants, including a tRNASerAGA G35A variant with a phenylalanine anticodon (tRNASerAAA) found in ∼2% of the population. The tRNAPro mutant caused synthetic toxicity with a deleterious huntingtin poly-glutamine (polyQ) allele in neuronal cells. The tRNASerAAA variant showed synthetic toxicity with proteasome inhibition but did not enhance toxicity of the huntingtin allele. Cells mistranslating phenylalanine or proline codons with serine had significantly reduced rates of protein synthesis. Mistranslating cells were slow but effective in forming insoluble polyQ aggregates, defective in protein and aggregate degradation, and resistant to the neuroprotective integrated stress response inhibitor (ISRIB). Our findings identify mistranslating tRNA variants as genetic factors that slow protein aggregation kinetics, inhibit aggregate clearance, and increase drug resistance in cellular models of neurodegenerative disease.
Of maps and grids
Authors: Grasso Matteo , Haun Andrew M , Tononi Giulio 
Published: September 2021

Abstract
Neuroscience has made remarkable advances in accounting for how the brain performs its various functions. Consciousness, too, is usually approached in functional terms: the goal is to understand how the brain represents information, accesses that information, and acts on it. While useful for prediction, this functional, information-processing approach leaves out the subjective structure of experience: it does not account for how experience feels. Here, we consider a simple model of how a “grid-like” network meant to resemble posterior cortical areas can represent spatial information and act on it to perform a simple “fixation” function. Using standard neuroscience tools, we show how the model represents topographically the retinal position of a stimulus and triggers eye muscles to fixate or follow it. Encoding, decoding, and tuning functions of model units illustrate the working of the model in a way that fully explains what the model does. However, these functional properties have nothing to say about the fact that a human fixating a stimulus would also “see” it—experience it at a location in space. Using the tools of Integrated Information Theory, we then show how the subjective properties of experienced space—its extendedness—can be accounted for in objective, neuroscientific terms by the “cause-effect structure” specified by the grid-like cortical area. By contrast, a “map-like” network without lateral connections, meant to resemble a pretectal circuit, is functionally equivalent to the grid-like system with respect to representation, action, and fixation but cannot account for the phenomenal properties of space.