bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low turbidity in recirculating aquaculture systems (RAS) reduces feeding behavior and increases stress-related physiological parameters in pikeperch (Sander lucioperca) during grow-out
Authors: Ende Stephan S W , Larceva Ekaterina , Bögner Mirko , Lugert Vincent , Slater Matthew James , Henjes Joachim 
Published: December 2021

Abstract
There is a tendency to farm fish in low turbidity water when production takes place in the land-based recirculating aquaculture systems (RAS). However, the effect of water turbidity on stress and performance is unknown for many species cultured in RAS. The effect of different turbidity treatments as Formazine Attenuation Units (0 FAU, 15 FAU, and 38 FAU) on feed intake performance (latency, total feeding time, and total feed intake) and physiological blood stress parameters (cortisol, lactate, and glucose) in medium-sized pikeperch ((Sander lucioperca) n = 27, undetermined sex and age) of initial body weights of 508.13 g ± 83 g (at FAU 0, 15, and 38, respectively) was investigated. The rearing system consisted of 9 rectangular tanks (200 L per tank). Fish were housed individually (n = 1, per tank, n replicates per treatment = 9). All tanks were connected to a recirculation system equipped with a moving bed biofilter. Feed intake in pikeperch kept at low turbidity (0 FAU) was 25% lower than pikeperch kept at high turbidity (38 FAU) (P &lt; 0.01) and also significantly (10.5%) lower compared to feed intake in pikeperch kept at intermediate turbidity (15 FAU) (P &lt; 0.01 for 0 FAU vs. 15 FAU, feed intake sign. Value as the main effect is P &lt; 0.01). Pikeperch kept at low turbidity showed significantly slower feeding response (latency time) towards pellets entering the tank, shorter feeding times (both P &lt; 0.05), and higher glucose blood concentration (73%) in contrast to pikeperch kept at highest turbidity. A reduction of 25% feed intake has obvious economic consequences for any fish farm and present data strongly emphasize the importance of considering the species-specific biology in future RAS farming.
Localized stem heating from the rest to growth phase induces latewood-like cell formation and slower stem radial growth in Norway spruce saplings
Authors: Giovannelli Alessio , Mattana Sara , Emiliani Giovanni , Anichini Monica , Traversi Maria Laura , Pavone Francesco Saverio , Cicchi Riccardo 
Published: December 2021

AbstractRecent climate projections predict a more rapid increase of winter temperature than summer and global temperature averages in temperate and cold environments. As there is relatively little experimental knowledge on the effect of winter warming on cambium phenology and stem growth in species growing in cold environments, the setting of manipulative experiments is considered of primary importance, and they can help to decipher the effect of reduced winter chilling and increased forcing temperatures on cambium reactivation, growth and xylem traits. In this study, localized stem heating was applied to investigate the effect of warming from the rest to the growth phase on cambium phenology, intra-annual stem growth dynamics and ring wood features in Picea abies (L.) H.Karst. We hypothesized that reduced winter chilling induces a postponed cambium dormancy release and decrease of stem growth, while high temperature during cell wall lignification determines an enrichment of latewood-like cells. The heating device was designed to maintain a +5 °C temperature delta with respect to air temperature, thus allowing an authentic scenario of warming. Continuous stem heating from the rest (November) to the growing phase determined, at the beginning of radial growth, a reduction of the number of cell layers in the cambium, higher number of cell layers in the wall thickening phase and an asynchronous stem radial growth when comparing heated and ambient saplings. Nevertheless, heating did not induce changes in the number of produced cell layers at the end of the growing season. The analyses of two-photon fluorescence images showed that woody rings formed during heating were enriched with latewood-like cells. Our results showed that an increase of 5 °C of temperature applied to the stem from the rest to growth might not influence, as generally reported, onset of cambial activity, but it could affect xylem morphology of Norway spruce in mountain environments.
Cutaneous Findings of Sporadic, Adult-Onset Neuronal Intranuclear Inclusion Disease
Authors: Moran Jakob M. T. , Eikermann-Haerter Katharina , Rapalino Otto , Dang Timothy M. , Holmes Robert K. , Chen Steven T. , Hoang Mai P. 
Published: December 2021

Abstract:
Neuronal intranuclear inclusion disease is a rare, progressive neurodegenerative disease whose hallmark histopathologic finding is the presence of ubiquitin-positive hyaline intranuclear inclusions in neuronal and non-neuronal cells. We present a case of neuronal intranuclear inclusion disease in a 61-year-old Asian man with a history of repeated episodes of altered mental status, long-standing bladder dysfunction, and cerebrovascular accidents. The patient had characteristic magnetic imaging findings of high signal along the cortico–medullary junction on diffusion-weighted sequences and symmetric T2 hyperintensity in the paravermal area of the cerebellum. Skin biopsies showed characteristic histopathologic findings of ubiquitin-positive intranuclear inclusions that ultrastructurally composed of filamentous material without limiting membrane within eccrine epithelium and dermal fibroblasts. Our case highlights the utility of readily accessible skin biopsy in the diagnosis of this rare neurodegenerative disease.
A Retrospective Study of SVF-gel Compared With Nanofat Combined With High-density Fat in the Treatment of Early Periorbital Aging
Authors: Zhu Yi , Yang Feng , Yang Yong , Cheng Biao , Zuo Jun 
Published: December 2021

Purpose:
To compare the effectiveness of transplantation with stromal vascular fraction (SVF)-gel or nanofat combined with high-density fat prepared with the Coleman technique (nanofat+high-density fat) to restore volume in the periorbital region or for periorbital rejuvenation in early periorbital aging.

Methods:
This retrospective study included 103 patients who received a transplant of SVF-gel (n = 58) or nanofat+high-density fat (n = 45) to restore volume in the periorbital region (n = 85) or for periorbital rejuvenation (n = 18) in our hospital between January 2016 and January 2020. Patient satisfaction and the reoperation rate were evaluated.

Results:
All patients had improved periorbital contouring and augmentation. Among the patients that received treatment to restore volume in the periorbital region, 17% and 65.9% of patients administered SVF-gel were very satisfied or satisfied, and 5.3% and 44.7% of patients administered nanofat+high-density fat were very satisfied or satisfied.

Patients
administered SVF-gel were significantly more satisfied than patients administered nanofat+high-density fat with improvements in periorbital contouring (p < 0.05). Among the patients that received treatment for periorbital rejuvenation, 54.5% and 27.3% of patients administered SVF-gel were very satisfied or satisfied, and 28.6% and 42.8% of patients administered nanofat+high-density fat were very satisfied or satisfied. There was no significant difference between groups (p > 0.05). Some patients underwent a second operation after 3 to 8 months. Patients administered SVF-gel to restore volume in the periorbital region had a significantly lower reoperation rate than patients administered nanofat+high-density fat (12.7% [6/47] vs. 34.2% [13/38]; p < 0.05). There was no significant difference in the reoperation rate in patients treated for periorbital rejuvenation (9.1% [1/11] vs. 14.3% [1/7]; p > 0.05).

Conclusion:
SVF-gel and nanofat+high-density fat are effective for restoring volume in the periorbital region and for periorbital rejuvenation in early periorbital aging. The reoperation rate was significantly lower and patient satisfaction scores were significantly higher in patients administered SVF-gel to restore volume in the periorbital region compared with patients administered nanofat+high-density fat.
Successful Treatment of Severe Pityriasis Rubra Pilaris with Cyclosporine A in An Infant
Authors: Chen Ping-Jiao , Liang Jing-Yao , Li Chang-Xing , Zhang Xi-Bao 
Published: December 2021

Abstract

Introduction:
Pityriasis rubra pilaris (PRP) is a rare chronic papulosquamous disease and is often difficult to treat, especially in children.
Here we reported a PRP infant successfully treated by cyclosporine A.

Case presentation:
An 18-month-old infant with severe PRP presented with reddish follicular papules and inflammatory, infiltrated erythema over the whole body. The condition rapidly and completely responded to oral cyclosporine A. Complete clearance of the lesions was achieved within 16 weeks, and no recurrence developed during 6 months of follow-up. During both treatment and follow-up, routine blood parameters, serum creatinine, serum urea nitrogen, liver function, and blood pressure were regularly monitored once a month and remained within the reference ranges. Moreover, the child grew and developed normally and the body weight increased by 3 kg.

Discussion:
To date, there are not well established protocols for treatment of PRP in children. Cyclosporine A has been demonstrated to be effective treatment in psoriasis in adults and children which do not respond to some other treatments. The rapid onset of action and good efficacy of cyclosporine A were observed in our case, who achieved complete response after 16 weeks of treatment without any side effects.

Conclusion:
Cyclosporine A might be a safe and effective option for treating severe PRP in children.
Identification of an eight-m6A RNA methylation regulator prognostic signature of uterine corpus endometrial carcinoma based on bioinformatics analysis
Authors: Miao Chenyun , Fang Xiaojie , Chen Yun , Zhao Ying , Guo Qingge 
Published: December 2021

Abstract
N6-methyladenosine (m6A) methylation is proved to play a significant role in human cancers. This study aimed to explore the association between m6A ribonucleic acid (RNA) methylation regulators and uterine corpus endometrial carcinoma (UCEC), and build a prognostic signature of m6A regulators for UCEC.
RNA-seq transcriptome data and clinicopathological data of UCEC were downloaded from the Cancer Genome Atlas database. We compared the expression of 23 m6A-regulators in tumor tissues and nontumor tissues. Then we classified the data into 3 clusters by consensus clustering analysis. Several regulators were picked out as the prognostic signature of patients with UCEC based on least absolute shrinkage and selection operator Cox regression analysis. Additionally, we established a predictive nomogram to calculate survival times. Finally, we used receiver operating characteristic curve, univariate Cox regression analysis, and multivariate Cox regression analysis to further verify the prognostic value of the risk signature consisting of m6A regulators.
The expression of 18/23 m6A regulators was significantly different in UCEC compared with normal samples. Gene ontology functional analysis of these regulators revealed that they were mainly participated in RNA splicing, stabilization, modification, and degradation. LRPPRC, IGFBP2, KIAA1429, IGFBP3, FMR1, YTHDF1, METTL14, and YTHDF2 were selected to construct the risk signature and predictive nomogram. The results of receiver operating characteristic curve, univariate Cox regression analysis, and multivariate Cox regression analysis for the risk signature showed a good predictive performance for UCEC.
The risk signature of 8-m6A regulators has potential prognostic value for patients with UCEC.
To explore the Radix Paeoniae Rubra-Flos Carthami herb pair's potential mechanism in the treatment of ischemic stroke by network pharmacology and molecular docking technology
Authors: Chen Xingyu , Wang Yue , Ma Ying , Wang Ruonan , Zhao Dexi 
Published: December 2021

AbstractTo explore the Radix Paeoniae Rubra-Flos Carthami herb pair's (RPR-FC) potential mechanism in treating ischemic stroke (IS) by network pharmacology and molecular docking technology.The Traditional Chinese Medicine Systems Pharmacology Database was used to screen the active components of the RPR-FC, and Cytoscape 3.8 software was used to construct a network map of its active components and targets of action. The GeneCards and OMIM databases were used to identify disease targets of IS, and the common targets were chosen as research targets and imported into the STRING database to construct a protein–protein interaction network map of these targets. R language software was used to analyze the enrichment of GO terms and KEGG pathways, and explore the mechanisms of these targets. Molecular docking technology was used to verify that the RPR-FC components had a good bonding activity with their potential targets.A total of 44 active components, which corresponded to 197 targets, were identified in the RPR-FC. There were 139 common targets between the herb pair and IS. GO functional enrichment analysis revealed 2253 biological process entries, 72 cellular components entries, and 183 molecular functions entries. KEGG pathway enrichment analysis was mainly related to the NF-kappa B signaling pathway, the TNF signaling pathway, apoptosis, the MAPK signaling pathway, the PI3K-Akt signaling pathway, the VEGF signaling pathway, etc. The molecular docking results showed the components that docked well with key targets were quercetin, luteolin, kaempferol, and baicalein.The active components (quercetin, luteolin, kaempferol, and baicalein) of the RPR-FC and their targets act on proteins such as MAPK1, AKT1, VEGFA, and CASP3, which are closely related to IS.1These targets are closely related to the NF-kappa B signaling pathway, the MAPK signaling pathway, the PI3K-Akt signaling pathway, the VEGF signaling pathway, and other signaling pathways. These pathways are involved in the recovery of nerve function, angiogenesis, and neuronal apoptosis and the regulation of inflammatory factors, which may have a therapeutic effect on IS.
Predicting outcomes after trauma
Authors: Lee Kuo-Chang , Lin Tzu-Chieh , Chiang Hsiu-Fen , Horng Gwo-Jiun , Hsu Chien-Chin , Wu Nan-Chun , Su Hsiu-Chen , Chen Kuo-Tai 
Published: December 2021

Abstract
In an overcrowded emergency department (ED), trauma surgeons and emergency physicians need an accurate prognostic predictor for critical decision-making involving patients with severe trauma. We aimed to develope a machine learning-based early prognostic model based on admission features and initial ED management.
We only recruited patients with severe trauma (defined as an injury severity score >15) as the study cohort and excluded children (defined as patients <16 years old) from a 4-years database (Chi-Mei Medical Center, from January 2015, to December 2018) recording the clinical features of all admitted trauma patients. We considered only patient features that could be determined within the first 2 hours after arrival to the ED. These variables included Glasgow Coma Scale (GCS) score; heart rate; respiratory rate; mean arterial pressure (MAP); prehospital cardiac arrest; abbreviated injury scales (AIS) of head and neck, thorax, and abdomen; and ED interventions (tracheal intubation/tracheostomy, blood product transfusion, thoracostomy, and cardiopulmonary resuscitation). The endpoint for prognostic analyses was mortality within 7 days of admission.
We divided the study cohort into the early death group (149 patients who died within 7 days of admission) and non-early death group (2083 patients who survived at >7 days of admission). The extreme Gradient Boosting (XGBoost) machine learning model provided mortality prediction with higher accuracy (94.0%), higher sensitivity (98.0%), moderate specificity (54.8%), higher positive predict value (PPV) (95.4%), and moderate negative predictive value (NPV) (74.2%).
We developed a machine learning-based prognostic model that showed high accuracy, high sensitivity, and high PPV for predicting the mortality of patients with severe trauma.
Loss of correction in cubitus varus deformity after osteotomy
Authors: You Chao , Zhou Yibiao , Han Jingming 
Published: December 2021

Abstract
We aimed to investigate the rate of loss of correction and the factors thereof in pediatric patients undergoing osteotomy for treatment of cubitus varus deformity.
Between July 2008 and July 2017, we treated 30 patients who underwent osteotomy for cubital varus. We compared the preoperative and postoperative clinical and imaging findings, including the H-Cobb and Baumman angles, in all patients. Postoperative evaluation was performed by telephonic interviews.
Our patients consisted of 17 males and 13 females. The mean age was 75 months. At the first follow-up, approximately 80% of patients had experienced a loss of correction of the humerus-cobb angle (H-Cobb angle); at the second follow-up, the incidence was 83%. Meanwhile, 57% and 43% of patients experienced a loss of correction of the Baumman angle at the first and second follow-ups, respectively. The average interval between the first and second follow-ups was 24 days, and the mean loss in the H-Cobb angle was 2.4°. There was a significant difference between the H-Cobb angles as measured before and after surgery (P < .05). There was no significant difference between the H-Cobb angles of the affected side and the contralateral healthy elbow at the third postoperative follow-up; however, there was a significant difference between the Baumman angle between before and after surgery (P < .05). The Baumman angles as measured at the second and third postoperative follow-ups differed significantly from those of the contralateral healthy elbow joint. According to the survival curve analysis, the median survival times of the H-Cobb and Baumman angles were 27 and 34 months, respectively.
The postoperative loss of the 2 angles occurred mainly during the first and second follow-up periods. Therefore, patient follow-up is particularly important in the period directly following the operation. Additional measures may be necessary to avoid rapid angle loss.
The efficacy and safety of modified Gegenqinlian Fomular for advanced colorectal cancer (damp heat accumulation type)
Authors: Wang Zhi-Jie , Wang Xiao-Han , Li Juan , Zheng Shi-Hang , Zhang Fu-Peng , Hao Shu-Lan , Wang Xi-Xing , Liu Li-Kun 
Published: December 2021

Abstract

Introduction:
CRC, the incidence of the fourth highest among males and the third among females, is one of the malignant tumors that seriously threaten human health. The principle of treatment for advanced stage CRC is a multidisciplinary and comprehensive treatment based on chemotherapy, which always bring significant toxic side effects. CHM has advantages in the treatment of tumors with the effect on improving clinical symptoms and reducing side effects. GGQL formula is mainly used for treating abnormal defecates caused by damp-heat, so we will evaluate the clinical efficacy and safety of modified GGQL formula for patients with advanced CRC with the type of damp-heat in this study.

Methods:
Multicenter RCT with two parallel groups in three hospitals planning to recruit 120 CRC patients with the type of damp-heat will be conducted. The control group will be treated by basic antitumor therapy and the treatment group will use modified GGQL formula plus basic antitumor therapy. The primary outcomes will be quality of life, TCM symptom score, PFS and OS, and the secondary outcomes will be performance status, size of tumor, tumor marker in the serum, tumor microenvironment and immune status. All analyses will be based on an intention-to-treat principle. This study was approved by the Human Research Ethics Committee of Shanxi Province Hospital of Traditional Chinese medicine (2021Y-06017). The results will be published in relevant journal.

Discussion:
The results of this RCT will contribute to Chinese herbal medicine for treating CRC patients with the type of damp heat accumulation.

Trial registration:
ChiCTR2100050754 (September 4, 2021).
Combination of preoperative CA19-9 levels, cell differentiation, and age predicts survival for patients with gastric cancer before surgery
Authors: Yin Hui Hui , Xu Meng Qing , Liu Bin Zheng , Tao Lin , Ma Ya Jing , Li Feng , Zhang Wen Jie 
Published: December 2021

Abstract
Gastric cancer (GC) is very common in China, posing a threat to public health, with high morbidity and mortality ranks. Tumor-node-metastasis (TNM) staging system is routinely used to predict prognosis for patients with GC but only available after surgery. Therefore, searching for markers that can predict prognosis of GC patients before surgery is desirable to assist management decisions preoperatively. Among 322 GC patients followed-up for 128 months, the tumor markers alpha fetoprotein, carcinoembryonic antigen, carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 15-3 and carbohydrate antigen 72-4 of 168 patients were detected before surgery, and their impact on survival was analyzed. Four major findings were revealed: (1) Preoperative examined CA19-9 levels and cell differentiation using endoscopic biopsies were positively correlated with lymphatic metastases and TNM stages obtained after surgery. (2) Kaplan-Meier analyses demonstrated that poor survival of patients with GC was associated with higher CA19-9 levels, poor cell differentiation, and older age. (3) Cox multi-factorial regression analyses indicated that, in terms of predicting overall survival for GC patients, preoperative CA19-9 level, cell differentiation and age were independent factors, respectively, comparable to postoperative TNM staging system. (4) Using receiver operating characteristic curve analysis, we first revealed that preoperative CA19-9 levels and cell differentiation had the impact weights (IW) on survival comparable to postoperative TNM components. These findings suggest that preoperative CA19-9 levels, cell differentiation and age are useful prognostic related markers for GC patients, superior to postoperative TNM system in terms of timing for management. We propose that, assisted by clinical imaging, a comprehensive utilization of these preoperative survival-predictors may help formulate individualized medical management for GC patients such as surgical strategy, optimal chemotherapy and radiotherapy, and appropriate follow-up intervals after surgery.