bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Complete Chloroplast Genome and Characteristics Analysis of Musa basjoo Siebold
Authors: Liu Fenxiang , Movahedi Ali , Yang Wenguo , Xu Dezhi , Jiang Chuanbei , Xie Jigang , Zhang Yu 
Published: June 2021

Abstract
Musa basjoo Siebold is an ornamental plant often seen in gardens and farmhouses. It can also be used as a kind of Chinese herbal medicine. The decoct soup of its pseudo stem can help relieve the heat. Its pseudo stem and leaves are diuretic. The decoct soup of its dried flower can treat cerebral hemorrhage. Its root can be decocted together with ginger and licorice to cure gonorrhea and diabetes. The chloroplast genome study on Musa basjoo Siebold is rarely seen. This paper showed that the length of the chloroplast genome Musa basjoo Siebold was 172,322 bp, with 36.45% GC content. Musa basjoo Siebold included a large single-copy region of 90,160 bp, a small single-copy region of 11,668 bp, and a pair of inverted repeats of 35,247 bp. Comparing the genomic structure and sequence data of closely related species, we revealed the conserved gene order of the IR and LSC/SSC regions, which provided a very inspiring discovery for future phylogenetic research. Overall, this study is the first time an evolutionary tree of the genus Musa species has been constructed with the complete chloroplast genome sequence. We can see that there is no obvious multi-branching in the genus, and Musa basjoo Siebold, and Musa itinerans are the closest relatives.
Evaluation of an Automatic Insulated Isothermal PCR System for Rapid and Reliable Field-Deployable Detection of African Swine Fever Virus
Authors: NGA TRAN THI HUYEN , NHI-CONG LE THI , PHUONG PHAM BANG , QUYNH LUU VAN , Nguyen Viet-Linh 
Published: June 2021

Abstract
In the present study, we evaluate an automatic sample-to-answer insulated isothermal PCR system for rapid and reliable field-deployable detection of African swine fever virus in comparison to that of OIE recommended real-time PCR counterparts with samples collected in Vietnam. For analytical sensitivity, the system could detect ASFV up to a dilution of 106 whereas the real time PCR systems could detect up to a dilution of 105 to 106. For specificity test, the system showed high specificity to ASFV in compare to different other types of swine pathogens: PRRSV, FMDV, PCV2, CSFV. The diagnostic performance comparison on 6 different types of samples showed 97.3% to 100% agreement with reference real-time PCR. The results of this study indicated that POCKIT Central-based insulated isothermal PCR system is a rapid, reliable and sample-flexible method for effective detection of ASFV.
N6-Methylandenosine-Related lncRNAs are Potential Biomarkers for Predicting the Overall Survival Rate of Patients Bladder Cancer
Authors: Zhang Haihang , Xie Panpan , Shi Kaijia , Xu Danni , Cai Xingrui , Yang Changcheng , Zeng Jiangzheng , Lu Yanda 
Published: June 2021

Abstract
Background: The prognostic value of N6-methylandenosine-related long non-coding RNAs (m6A-related lncRNAs) was investigated in 414 bladder cancer (BLCA) and 19 normal bladder tissue samples from The Cancer Genome Atlas (TCGA) datasets. Methods: We implemented Pearson correlation analysis to explore the lncRNAs associated with m6A, and then performed univariate Cox regression analysis to identify nine m6A-associated lncRNAs with prognostic value. The patients with BLCA were divided into two subgroups by consistency clustering. Analysis of the two groups of immune cell infiltration, immune microenvironment and clinical results were significantly different. We identified the prognostic significance of m6A-related lncRNAs by bioinformatic and statistical analysis of data from patients with BLCA.Results: We constructed an m6A-related lncRNA prognostic signature (m6A-LPS, including AL136295.2, AC104564.3, ATP1B3-AS1, EHMT2-AS1 and AC116914.2) to predict the OS of BLCA patients. It is proved that m6A LPS has independent prognostic value.
The Impacts of Integrated Health Hydroponic Planting On Green Space Exposure, Happiness, Anxiety and Stress Among Secondary School Students: A Feasibility Quasi-Experiment
Authors: Kwok Stephen Wai Hang , Mak Yim Wah , Chiu Alice Pui Ying , Chong Yan Kit , Suen Lorna Kwai Ping , Kam Edmond , Wong Cecilia K.C. , Wu Cynthia Sau Ting 
Published: June 2021

Abstract
Background: Mental health of adolescents have been exposing to risk factors associated with stress from a variety of sources. Previous studies identified that green space exposure might be effective for promoting mental health and reduction of stress. However, previous literature focused on adults, and for those which studied children and adolescents, the effects of planting and similar green activities remain to be explored. The aim of this study was to investigate the effects of a pioneer school-based program on green space use and satisfaction, and mental wellbeing indicators, among early adolescent students. Methods: This was a feasibility quasi-experiment in which intervention and control groups were studied sequentially. Three secondary schools and 55 grade 7-8 students were sampled conveniently. In the intervention school, the green space program consists of learning hydroponic planting and group practice in creating green areas at school. In the two control schools, the activities consisted of a standardized stress management program including relaxation exercise such as stretching, extension and massage. Both groups learned green diet, tasting and had green living activity, stress awareness games and learned stress coping. The activity schedule for each group (intervention and control) consisted of three one-hour sessions, each was conducted two to three weeks apart. Key outcomes were assessed with questionnaires at baseline and one month after the completion of the activity period as for each school. Data were fit with mixed model applying GEE. Results: At baseline, students were not sure whether they know how to plant herbs or prepare herbal tea. Control group at posttest had lower frequencies of using green facilities in the community and using school gardening facilities in the previous four weeks compared with intervention group and pretest. Females at posttest were associated with higher confidence in planting, and higher level of enjoyment from green activities. Other moderate to small but non-significant effects were also identified. Conclusions: In this study, the school-based green space programme was found feasible for early adolescent students with statistically supported results. Limitations have also been identified and future study with larger scale, more frequent intervention, longer follow-up and RCT design are required.
Lesion of The Hippocampus Selectively Enhances LEC's Activity During Recognition Memory Based On Familiarity
Authors: Mahnke Liv , Atucha Erika , Pina-Fernàndez Eneko , Kitsukawa Takashi , Sauvage Magdalena M. 
Published: June 2021

Abstract
The sense of familiarity for events is crucial for successful recognition memory. However, the neural substrate and mechanisms supporting familiarity remain unclear. A major controversy in memory research is whether the parahippocampal areas, especially the lateral entorhinal (LEC) and the perirhinal (PER) cortices, support familiarity or whether the hippocampus (HIP) does. In addition, it is unclear if LEC, PER and HIP interact within this frame. Here, we especially investigate if LEC and PER's contribution to familiarity depends on hippocampal integrity. To do so, we compare LEC and PER neural activity between rats with intact hippocampus performing on a human to rat translational task relying on both recollection and familiarity and rats with hippocampal lesions that have been shown to then rely on familiarity to perform the same task. Using high resolution Immediate Early Gene imaging, we report that hippocampal lesions enhance activity in LEC during familiarity judgments but not PER’s. These findings suggest that different mechanisms support familiarity in LEC and PER and led to the hypothesis that HIP might exert a tonic inhibition on LEC during recognition memory that is released when HIP is compromised, possibly constituting a compensatory mechanism in aging and amnesic patients.
Richards&#39; equation reconsidered
Authors: Vodák Rostislav , Fürst Tomáš , Šír Miloslav , Kmec Jakub 
Published: June 2021

Abstract
Semi-continuum modelling of unsaturated porous media flow is based on representing the porous medium as a grid of non-infinitesimal blocks that retain the character of a porous medium. Semi-continuum model is able to physically correctly describe diffusion-like flow, finger-like flow, and the transition between them. This article presents the limit of the semi-continuum model as the block size goes to zero. In the limiting process, the retention curve of each block scales with the block size and in the limit becomes a hysteresis operator of the Prandtl-type used in elasto-plasticity models. Mathematical analysis showed that the limit of the semi-continuum model is a partial differential equation with a hysteresis operator of Prandl's type. This limit differs from the standard Richards' Equation (RE), which is not able to describe finger-like flow.
Since the physics behind both RE and the semi-continuum model is almost the same, we suggest a way to reformulate the RE so that it retains the ability to describe finger-like flow. We conclude that RE should be reconsidered by means of appropriate modelling of the hysteresis and correct scaling of the retention curve.
Potential of Prevention Strategies for the Modifiable Risk Factor Type 2 Diabetes on the Future Number of Dementia Patients in Germany– A Multi-State Projection Through 2040
Authors: Fink Anne , Demuth Ilja , Doblhammer Gabriele 
Published: June 2021

Abstract
Background: We assess the impact of prevention strategies regarding type 2 diabetes as a modifiable risk factor for dementia and its consequences for the future number of dementia patients in Germany. Methods: We used a random sample of health claims data (N=250,000) of insured persons aged 50+ drawn in 2014, and data on population size and death rates in 2015 from the Human Mortality Database. Using exponential hazard models, we calculated age- and sex-specific transition probabilities and death rates between the states (no diabetes/no dementia, diabetes/no dementia, no diabetes/dementia, diabetes/dementia). In multi-state projections, we estimated the future number of dementia cases aged 75+ through 2040 depending on the development of the incidence of diabetes among persons without diabetes and without dementia, and the dementia incidence among persons with and without diabetes. Results: In 2015 there were 1.34 million people with dementia aged 75+ in Germany. A relative annual reduction in death rates of 2.5% will increase this number to 2.68 million by 2040. A relative reduction of diabetes incidence by 1% annually would decrease dementia cases by around 26,000, while a reduction of dementia incidence among people with diabetes by 1% would result in 175,000 fewer dementia cases. Both prevention strategies combined would prevent 191,000 dementia cases in 2040.Conclusions: The increase in life expectancy is decisive for the future number of people with dementia. Strategies of better diabetes treatment have the potential to lower the number of dementia patients in the coming decades.
Quantifying Root Colonization in Arbuscular Mycorrhizas by Image Segmentation and Machine Learning
Authors: Sciascia Ivan Arcangelo , Andrea Crosino , Novero Mara , Politi Mara , Genre Andrea 
Published: June 2021

Abstract
MotivationArbuscular mycorrhizas are the most widespread plant symbioses and involve the majority of crop plants. The beneficial interaction between plant roots and a group of soil fungi (Glomeromycotina) grants the green host a preferential access to soil mineral nutrients and water, supporting plant health, biomass production and resistance to both abiotic and biotic stresses. The nutritional exchanges at the core of this symbiosis take place inside the living root cells, which are diffusely colonized by specialized fungal structures called arbuscules. For this reason, the vast majority of studies investigating arbuscular mycorrhizas and their applications in agriculture require a precise quantification of the intensity of root colonization. To this aim, several manual methods have been used for decades to estimate the extension of intraradical fungal structures, mostly based on optical microscopy observations and individual assessment of fungal abundance in the root tissues. ResultsHere we propose a novel semi-automated approach to quantify AM colonization based on digital image analysis and compare two methods based on image thresholding and machine learning. Our results indicate in machine learning a very promising tool for accelerating, simplifying and standardizing this critical type of analysis, with a direct potential interest for applicative and basic research.Contactivan.sciascia@unito.it; andrea.genre@unito.it
Seamens’ Sign: A Novel Electrocardiogram Prediction Tool for Left Ventricular Hypertrophy
Authors: Walker Philip , Jenkins Cathy , Hatcher Jeremy , Freeman Clifford , Sr Nickolas , Rosell Bryant , Hanna Eriny , March Cooper , Robinson Taylor , Wollenman Lucas , Pfister Tyler , Pope Rand , Azose Aaron , Henry Olivia , O'Shea Jessica , Kunnath Ansley , Storrow Alan , Seamens Charles , McCoin Nicole 
Published: June 2021

Abstract
Study Objective:

We developed a novel, quick, easy to use electrocardiogram (ECG) screening criterion (Seamens’ Sign) for left ventricular hypertrophy (LVH). This new criterion was defined as the presence of QRS complexes touching or overlapping in two contiguous precordial leads.
Methods:

This study was a retrospective chart review of 2184 patient records. The primary outcome was whether Seamens’ Sign was noninferior in confirming LVH compared to other common criteria. Test characteristics were calculated for each of the LVH criteria. Inter-rater agreement was assessed on a random sample using Cohen’s Kappa.
Results:

Median age was 63, 52% of patients were male and there was a 35% prevalence of LVH by transthoracic echocardiogram (TTE). Nine percent were positive for LVH on ECG based on Seamens’ Sign. Seamens’ Sign had a specificity of 0.92. Tests assessing noninferiority indicated Seamens’ Sign was non-inferior to all criteria (p < 0.001) except for Cornell criterion for women (p = 0.98). Seamens’ Sign had 90% (0.81-1.00) inter-rater agreement, the highest of all criteria in this study.
Conclusion:

When compared to both the Sokolow-Lyon criteria and the Cornell criterion for men, Seamens’ Sign is noninferior in ruling in LVH on ECG. Additionally, Seamens’ Sign has higher inter-rater agreement compared to both Sokolow-Lyon criteria as well as the Cornell criteria for men and women, perhaps related to its ease of use.
The Effect of Early Mobilisation on the Sagittal Alignment and Clinical Outcome Following Conservative Treatment of the AO A3 and A4 Thoracolumbar Spine Fracture in Comparison to A1 Fractures. A Retrospective Study
Authors: Elshamly Mahmoud , Toegel Stefan , Grohs Josef G 
Published: June 2021

Abstract
BackgroundThe aim of the study was to correlate the clinical and radiological outcomes following the conservative treatment of neurologically intact patients with AO A4, A3, and A1 thoracolumbar (TL) fractures.MethodsRetrospective study included 3 cohorts of conservatively treated patients with AO A4, A3, and A1 TL fracture without the use of bracing or casting. At the final follow up segmental kyphotic angle (SKA), regional lordotic angle (RLA), lordosis gap (LG), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), sagittal vertical axis (SVA), lumbar lordosis (LL), thoracic kyphosis (TK), and femoral obliquity angle (FOA), and the Oswestry disability index (ODI) were assessed. Data were analyzed using descriptive statistics, non-parametric inferential statistics, and Spearman correlation analyses.ResultsAge was significantly higher in A4 group than in A1 group (p=0.04). The median 1ry SKA of the A3 group (15 ± 3) was significantly higher than in A1 group (7 ± 7, p=0.04). The median of total ODI in the A4 group (42 ± 53) and A3 group (31.3 ± 27) was clinically higher than in A1 group (11.1 ± 25), however, this difference was not statistically significant. Age as well as SVA correlated significantly with PT, FOA, SKA at the follow up, and the total ODI.ConclusionAge of the patient is a significant confounder that has an important impact on the type of fracture, sagittal malalignment, its compensatory mechanisms, and the resulting clinical outcome following conservative treatment of AO A4 and A3 TL fracture.
In-Vivo Staging of Regional Amyloid Progression In Healthy Middle Aged To Older People At Risk of Alzheimer’s Disease
Authors: Levin Fedor , Jelistratova Irina , Betthauser Tobey J. , Okonkwo Ozioma , Johnson Sterling C. , Teipel Stefan J. , Grothe Michel J. 
Published: June 2021

Abstract
Background We investigated regional amyloid staging characteristics in 11C-PiB-PET data from middle-aged to older participants at elevated risk for AD enrolled in the Wisconsin Registry for Alzheimer’s Prevention. Methods We analyzed partial volume effect-corrected 11C-PiB-PET distribution volume ratio maps from 220 participants (mean age = 61.4y, range: 46.9-76.8y). Regional amyloid-positivity was established using region-specific thresholds. We used four stages from frequency-based staging of amyloid-positivity to characterize individual amyloid deposition. Longitudinal PET data was used to assess temporal progression of stages and to evaluate emergence of regional amyloid-positivity in participants who were amyloid-negative at baseline. We also assessed the effect of amyloid stage on longitudinal cognitive trajectories.Results The staging model suggested progressive accumulation of amyloid from associative to primary neocortex and gradually involving subcortical regions. Longitudinal PET measurements supported the cross-sectionally estimated amyloid progression. In mixed-effects longitudinal analysis of cognitive follow-up data obtained over an average period of 6.5 years following the baseline PET measurement, amyloid stage II showed a faster decline in executive function and advanced amyloid stages (III and IV) showed a faster decline across multiple cognitive domains compared to stage 0.Conclusions Overall, the 11C-PiB-PET-based staging model was generally consistent with previously derived models from 18F-labled amyloid PET scans and a longitudinal course of amyloid accumulation. Differences in longitudinal cognitive decline support the potential clinical utility of in-vivo amyloid staging for risk stratification of the preclinical phase of AD even in middle-aged to older individuals at risk for AD.
Comparison of Sampling Methods in Assessing the Microbiomefrom Patients with Ulcerative Colitis
Authors: Kim Dan , Jung Jun-Young , Oh Hyun-Seok , Jee Sam-Ryong , Park Sung Jae , Lee Sang-Heon , Yoon Jun-Sik , Yu Seung Jung , Yoon In-Cheol , Lee Hong Sub 
Published: June 2021

Abstract
Background: Dysbiosis of ulcerative colitis (UC) has been frequently investigated using readily accessible stool samples. However, stool samples might insufficiently represent the mucosa-associated microbiome status. We hypothesized that luminal contents including loosely adherent luminal bacteria after bowel preparation may be suitable for diagnosing the dysbiosis of UC.Methods: This study included 16 patients with UC (9 men and 7 women, mean age: 52.13 ± 14.09 years) and 15 sex- and age-matched healthy individuals (8 men and 7 women, mean age: 50.93 ± 14.11 years). They donated stool samples before colonoscopy and underwent luminal content aspiration and endoscopic biopsy during the colonoscopy. Then, the composition of each microbiome sample was analyzed by 16S rRNA-based next-generation sequencing.Results: The microbiome between stool, luminal contents, and biopsy was significantly different in alpha and beta diversities. However, a correlation existed between stool and luminal contents in the Procrustes test (p = 0.001) and Mantel test (p = 0.0001). The stool microbiome was different between patients with UC and the healthy controls. Conversely, no difference was found in the microbiome of luminal content and biopsy samples between the two subject groups. The microbiome of stool and lavage predicted UC, with AUC values of 0.85 and 0.81, respectively.Conclusion: The microbiome of stool, luminal contents, and biopsy was significantly different. However, the microbiome of luminal contents during colonoscopy can predict UC, with AUC values of 0.81. Colonoscopic luminal content aspiration analysis could determine microbiome differences between patients with UC and the healthy control, thereby beneficial in screening dysbiosis via endoscopy. Trial registration: This trial was registered at http://cris.nih.go.kr.Registration No.: KCT0003352), Date : 2018-11-13
Exosomal Micro RNA-107 Reverses Chemotherapeutic Drug Resistance of Gastric Cancer Cells Through HMGA2/mTOR/P-gp Pathway
Authors: Jiang Lu , Zhang Yan , Guo Linghui , Liu Chaoyang , Ren Weihong 
Published: June 2021

Abstract
Background: RNA cargo in exosomes，especially microRNAs (miRNAs), play an important role in the chemotherapy drug resistance of human cancers. However, the role and mechanism of exosomal miR-107 on multidrug resistance of gastric cancer cells was still not clear. In this study, we sought to explore whether exosomal miR-107 could reverse theresistance of gastric cancer cells to the chemotherapy drugs. Methods: We extracted exosomes from sensitive (SGC-7901, MGC-803) and resistant (SGC-7901/5-FU) gastric cancer cells by ultracentrifugation and the isolated exosomes were identified using transmission electron microscopy (TEM) and dynamic light scattering analysis (DLS). The expression of miR-107 and high mobility group A2 (HMGA2) were detected by real-time quantitative PCR (RT-qPCR). MTT assay was used to investigate the effect of exosomes on gastric cancer cells growth in vitro. The uptake of exosomes by recipient cells were observed using a fluorescence microscope. The predicted target relationship between miR-107 and HMGA2 was verified by gauss-luciferase reporter assay. The expression of HMGA2, p-mTOR/mTOR, P-gp and other exosomal indicated marker proteins were detected by western blot. Results: Our results indicated that the isolated exosomes were demostrated typically cup-like lipid bilayer membrans structure. SGC-7901/5-FU cells were cross-resistant to chemotherapy drug cisplatin (DDP), and the sensitive cells-secreted exosomes drastically reversed the resistance of the resistant gastric cells to the chemotherapeutic drugs,which was verificated by exosomal inhibitor GW4896. Mechanistically, the reversal effect were mainly mediated by exosome-secreted miR-107 through downregulating the expression of targert molecular HMGA2, and inhibiting HMGA2/mTOR/P-gp pathway, which were proofed by luciferase reporter assay and rescue assay. Conclusions: These findings demonstrated that exosome-transmitted miR-107 significantly enhanced the sensitivity of resistant gastric cancer cells to chemotherapeutic agents by mediating the HMGA2/mTOR/P-gp axis and appling exosomal miR-107 may be a novel target in gastric cancers treatment.