bluuebunny/crossref_metadata_2025_split huggingface dataset:
НОВИЙ ПАРТЕНОКАРПІЧНИЙ ГІБРИД ОГІРКА СЛАВА F1 ДЛЯ УМОВ ЗАХИЩЕНОГО ҐРУНТУ
Authors: Sergienko O. V. , Solodovnik L. D. , Garbovska T. M. , Radchenko L. O. 
Published: August 2021

У вирішенні питання щодо повного забезпечення потреб споживчого ринку велике значення має розширення та збільшення надходження вітчизняного сортименту культури огірка. Саме тому на сьогодні актуальним є створення партенокарпічних гібридів F1 огірка корнішонного типу зі стійкістю до основних хвороб адаптованих до умов плівкових теплиць. Мета – на основі науково-методичного обґрунтування та добору стійких батьківських форм створити партенокарпічний гетерозисний гібрид F1 огірка корнішонного типу жіночого типу цвітіння, з високою урожайністю та товарністю плодів, стійкий до кореневих гнилей і пероноспорозу призначений для вирощування у плівкових теплицях. Методи. Польові, лабораторні, статистичні. Дослідження проводили в Інституті овочівництва і баштанництва НААН в умовах захищеного ґрунту. Результати. У результаті селекційної роботи створено новий середньоранній гібрид огірка Слава F1 із урожайністю 24,1 кг/м2, товарністю 95 %, період плодоношення якого складає 54 діб, партенокарпічного типу (73-85 %), стійкий до хвороб. Середня маса плоду – 74 г. Плід – зеленець короткий (8–9 см), середній за діаметром (2,0–3,2 см). Хімічний склад: вміст сухої речовини – 7,16 %, загального цукру – 2,20 %, вітаміну С – 9,47 мг/100 г. Дегустаційна оцінка свіжих плодів – 9,0 балів, солоних – 8,7. Вирощування нового гібрида забезпечує економічний ефект 66 грн/м2. Висновки. Гібрид рекомендується для розширення сортименту при вирощуванні у захищеному ґрунті у весняно-літній культурі в плівкових та скляних з обігрівом і без обігріву теплицях для сільськогосподарських підприємств різних форм власності та господарювання, переробних підприємств та приватного сектору в усіх зонах України. Гібрид передано на кваліфікаційну експертизу до Державної служби з охорони прав на сорти рослин.
ФАКТОРЫ ПОВЫШЕНИЯ ЭФФЕКТИВНОСТИ ТЕХНОЛОГИИ ВЫРАЩИВАНИЯ И ПЕРЕРАБОТКИ ГРИБОВ РОДА ВЕШЕНКА PLEUROTUS (FR.) P. KUMM
Authors: Bandura I. I. , Kulyk A.S. , Khareba О. V. , Khareba V. V. , Kovtuniuk Z. І. 
Published: August 2021

Грибы рода вешенка Pleurotus (Fr.) P. Kumm являются источником биологически активных веществ, функциональность которых подтверждена многократно научными исследованиями. К сожалению, отсутствуют данные о динамике изменения биохимического состава в процессе созревания плодовых тел. Эти данные могли бы определить оптимальное время сбора урожая и направления переработки, позволяющие максимально сохранить питательную ценность грибного сырья. С другой стороны, для эффективного производства и переработки этих грибов необходимо исследовать органолептические показатели штаммов с высокой урожайностью, а также технологические особенности их первичной переработки. Изучены характеристики 6 штаммов вешенки, относящихся к двум видам P. ostreatus (Fr.) P. Kumm и P. pulmonarius (Fr.) Quél, которые были разделены на две группы по оптимуму температуры выращивания: 12…16°С – группа А и 19…24°С – группа В. В группах определены наиболее продуктивные штаммы: 2316 (А) с показателем биологической эффективности – 78,9 % и 431 (В) – 78,4 %. Проведена органолептическая оценка плодовых тел после пятиминутной термической обработки, в результате которой выделены штаммы 2301 и Z (А), имеющие насыщенную окраску и нежную текстуру плодовых тел. Плодовые тела штамма 2314 (В) отличались ярким грибным ароматом и приятным вкусом. Найдены коэффициенты потери грибного сырья на двух этапах первичной переработки: инспектирования и бланширования. Оказалось, что потери при очистке не превышают 10%, но имеют разные значения для сростков, собранных на стадии технической и биологической зрелости. Инспектирование сростков штамма 2314 (А) проходило с минимальными потерями (1–2 %). Бланширование зрелых плодовых тел не приводило к уменьшению массы сырья, тогда как при термической обработке грибов технической зрелости потери составляли от 3 до 7 %. Исследование биохимического состава плодовых тел на разных стадиях морфогенеза позволило выявить тенденцию снижения содержания сухих веществ, протеинов и увеличения количества зольных элементов в зрелых плодовых телах изученных штаммов.
ПОЛІТИЧНА УЧАСТЬ В УМОВАХ ПАРТИЦИПАТОРНОЇ ТА ДОРАДЧОЇ ДЕМОКРАТІЇ: НОВІТНІ ПІДХОДИ, СУЧАСНЕ ТРАКТУВАННЯ
Authors: Косілова О.І. , Федірко І. П. 
Published: August 2021

Стаття присвячена проблемі участі громадян у прийнятті політичних рішень в умовах розвитку демократії. Аналізуються новітні форми демократії – партисипаторна і дорадча ‒ та особливості політичної участі в ній. Досліджується парадокс сучасної демократії, у якій, незважаючи на зростання кількості форм участі громадян, збільшується бар’єр між пересічними громадянами та владою, виникає проблема політично го відчуження та абсентеїзму. Розглядаються шляхи подолання цих проблем і різні форми участі громадськості у прийнятті політичних рішень шляхом реалізації політичних прав і свобод.
Каторжні тюрми України у контексті реформування пенітенціарної системи Російської імперії (друга половина ХІХ – початок ХХ ст.)
Authors: Bilichenko Lidiia 
Published: August 2021

У статті досліджено реформування системи каторжних тюрем у Російській імперії другої половини ХІХ – початку ХХ ст. Встановлено, що виникнення каторжних тюрем на території України пов’язано з пенітенціарною реформою імператора Олександра ІІ 1869 р., у результаті якої в Харківській губернії було створено Новоборисоглібський і Новобєлгородський каторжні централи. Доведено, що основною причиною відкриття каторжних тюрем в Україні стало зростання революційного руху та збільшення кількості засуджених осіб до каторжних робіт за революційну пропаганду. У результаті пенітенціарної реформи 1906-1907 рр. на території України було відкрито Харківську, Херсонську та Миколаївську каторжні тюрми.
Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis
Authors: Lu Haiping , Zhang Yi , Xiong Shan , Zhou Yinghong , Xiao Lan , Ma Yaping , Xiao Yin , Wang Xin 
Published: August 2021

Background: As a wound dressing and barrier membrane, surface modification of polycaprolactone (PCL) is needed in order to achieve better biological activities. Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue regeneration promise. Silver nanoparticles (Ag) have been suggested as the surface modification technique for various medical devices.Materials and Methods: Ag and human bone marrow MSC (hBMSC)-derived exosomes (MSCs-exo) were used to modify the PCL scaffold. The impact of different scaffolds on immune cells and MSC proliferation and differentiation was further evaluated.Results: MSCs-exo exhibited cup-shaped morphology with a diameter around 100 nm. MSCs-exo were enriched with exosome marker CD81 and showed good internalization into recipient cells. 200 ng/ml Ag nanoparticles and MSCs-exo were further used to modify the PCL scaffold. The internalization study further indicated a similar releasing pattern of exosomes from Ag/MSCs-exo hybrid scaffolds into RAW264.7 and hBMSCs at 12 and 24 h, respectively. Macrophages play an important role during different stages of bone regeneration. The MTT and confocal microscopy study demonstrated no significant toxicity of exosome and/or Ag hybrid scaffolds for macrophages and MSCs. Inflammatory macrophages were further used to mimic the inflammatory environment. A mixed population of elongated and round morphology was noted in the exosome and Ag hybrid group, in which the proinflammatory genes and secretion of IL-6 and TNF-α were significantly reduced. In addition, the exosome and Ag hybrid scaffolds could significantly boost the osteogenic differentiation of hBMSCs.Discussion: This study highlights the possibility of using Ag nanoparticles and MSCs-exo to modify the PCL scaffold, thus providing new insight into the development of the novel immunomodulatory biomembrane.
Pericardial Effusion During the Perioperative Period for Left Atrial Appendage Closure
Authors: Yang Lifan , Zhang Xiaochun , Jin Qinchun , Kong Dehong , Zhang Yuan , Li Mingfei , Zhang Lei , Chen Shasha , Pan Wenzhi , Zhou Daxin , Ge Junbo 
Published: August 2021

Objectives: To analyze the predictors of pericardial effusion (PE) during the perioperative period of the left atrial appendage closure procedure in our center.Methods: A total of 624 consecutive patients with non-valvular AF undergoing LAAC from May 2014 to October 2019 were involved in this study. Patients were divided into groups depending on whether they showed no PE, intraoperative PE or postoperative PE. We analyzed the predictors of PE during the perioperative period of the LAAC procedure.Results: (1) Of the 624 patients in our population (age 68.2 ± 9.1 years, 63% male, CHA2DS2-VASc score 3.4 ± 1.6), 30 patients experienced PE in the perioperative period, including 10 intraoperative PE and 20 postoperative PE. (2) A total of 26 (86.6%) patients had mild PE. 4 (13.4%) patients had pericardial tamponade, 2 (6.7%) of which were intraoperative, and the other 2 (6.7%) postoperative. (3) Significant differences were measured in relation to female sex, intraoperative time, combined procedures, changes in sinus rhythm, device retrieval times and duration of hospitalization between 2 groups (no PE occurred, intraoperative PE), P values were 0.039, 0.024, 0.004, 0.015, 0.003 and 0.039.Conclusions: Female sex, paroxysmal AF, changing in sinus rhythm, device retrieval times and intraoperative time all had a positive association with PE during the perioperative period.
Heart Failure With Midrange Ejection Fraction: Prior Left Ventricular Ejection Fraction and Prognosis
Authors: Zhang Xinxin , Sun Yuxi , Zhang Yanli , Chen Feifei , Zhang Shuyuan , He Hongyan , Song Shuang , Tse Gary , Liu Ying 
Published: August 2021

Aims: Evidence-based guidelines for heart failure management depend mainly on current left ventricular ejection fraction (LVEF). However, fewer studies have examined the impact of prior LVEF. Patients may enter the heart failure with midrange ejection fraction (HFmrEF) category when heart failure with preserved ejection fraction (HFpEF) deteriorates or heart failure with reduced ejection fraction (HFrEF) improves. In this study, we examined the association between change in LVEF and adverse outcomes.Methods: HFmrEF patients with at least two or more echocardiograms 3 months apart at the First Affiliated Hospital of Dalian Medical University between September 1, 2015 and November 30, 2019 were identified. According to the prior LVEF, the subjects were divided into improved group (prior LVEF &lt; 40%), stable group (prior LVEF between 40 and 50%), and deteriorated group (prior LVEF ≥ 50%). The primary outcomes were cardiovascular death, all-cause mortality, hospitalization for worsening heart failure, and composite event of all-cause mortality or all-cause hospitalization.Results: A total of 1,168 HFmrEF patients (67.04% male, mean age 63.60 ± 12.18 years) were included. The percentages of improved, stable, and deteriorated group were 310 (26.54%), 334 (28.60%), and 524 (44.86%), respectively. After a period of follow-up, 208 patients (17.81%) died and 500 patients met the composite endpoint. The rates of all-cause mortality were 35 (11.29%), 55 (16.47%), and 118 (22.52%), and the composite outcome was 102 (32.90%), 145 (43.41%), and 253 (48.28%) for the improved, stable, and deteriorated groups, respectively. Cox regression analysis showed that the deterioration group had higher risk of cardiovascular death (HR: 1.707, 95% CI: 1.064–2.739, P = 0.027), all-cause death (HR 1.948, 95% CI 1.335–2.840, P = 0.001), and composite outcome (HR 1.379, 95% CI 1.096–1.736, P = 0.006) compared to the improvement group. The association still remained significant after fully adjusted for both all-cause mortality (HR = 1.899, 95% CI 1.247–2.893, P = 0.003) and composite outcome (HR: 1.324, 95% CI: 1.020–1.718, P = 0.035).Conclusion: HFmrEF patients are heterogeneous with three different subsets identified, each with different outcomes. Strategies for managing HFmrEF should include previously measured LVEF to allow stratification based on direction changes in LVEF to better optimize treatment.
Clinical Efficacy and Safety of Cox-Maze IV Procedure for Atrial Fibrillation in Patients With Hypertrophic Obstructive Cardiomyopathy
Authors: Meng Yanhai , Zhang Yanbo , Liu Ping , Zhu Changsheng , Lu Tao , Hu Enci , Yang Qiulan , Nie Changrong , Wang Shuiyun 
Published: August 2021

Objective: Atrial fibrillation is the most prevalent persistent arrhythmia in patients with hypertrophic obstructive cardiomyopathy. Comparative analyses of the safety and effectiveness of septal myectomy with and without surgical ablation are limited. This study aimed to compare the outcomes of septal myectomy with and without the Cox-maze IV procedure in patients with hypertrophic obstructive cardiomyopathy and atrial fibrillation.Methods: Ninety-four patients with hypertrophic obstructive cardiomyopathy and atrial fibrillation who underwent septal myectomy were analyzed, we divided it into concomitant Cox maze surgery (Cox-maze group) and no concomitant Cox maze operation (no Cox-maze group). Freedom from atrial fibrillation recurrence and all-cause mortality after surgery were assessed.Results: Freedom from all-cause mortality after septal myectomy at 1, 3, and 5 years was 98.5 ± 1.5% each in the Cox-maze group and 90.8 ± 6.3%, 85.1 ± 8.1%, and 85.1 ± 8.1%, respectively, in the no Cox-maze group. Patients in the no Cox-maze group had lower survival, freedom from atrial fibrillation recurrence off antiarrhythmic drugs, and arrhythmia control rate (including patients with successful antiarrhythmic drug conversion) than those in the Cox-maze group (P = 0.046, P = 0.040, and P = 0.012, respectively). Patients who underwent the Cox-maze IV procedure had lower atrial fibrillation recurrence rate than those who did not (hazard ratio, 0.141; 95% confidence interval, 0.042–0.479; P = 0.002). Post-operative increases in left atrial diameters (hazard ratio, 1.099; 95% confidence interval, 1.024–1.179; P = 0.009) were associated with atrial fibrillation recurrence.Conclusions: The Cox-maze IV procedure combined with septal myectomy improved mid-term survival and reduced mid-term atrial fibrillation recurrence in patients with hypertrophic obstructive cardiomyopathy and atrial fibrillation. The concomitant Cox-maze IV procedure was associated with a lower atrial fibrillation recurrence in patients with surgical hypertrophic obstructive cardiomyopathy and atrial fibrillation.
Determination and Occurrence of Mineralocorticoids in Taihu Lake of China
Authors: Zhao Li , Chang Hong , Sun Fuhong , Su Hailei 
Published: August 2021

We developed a sensitive method for monitoring six natural (aldosterone) and synthetic mineralocorticoids (canrenone, spironolactone, 7β-spironolactone, 7α-thio spironolactone, and 7α-thiomethyl spironolactone) in sediment and water using ultra-performance liquid chromatography–electrospray tandem mass spectrometry, and then 30 water and 30 sediment samples were analyzed to reveal their occurrence and distributions in Taihu Lake. All target six mineralocorticoids were detected in sediment and water samples with the detection frequencies as high as 96–100%. The median concentrations of mineralocorticoids ranged from 0.04 ng/L (7α-thiomethyl spironolactone) to 14 ng/L (aldosterone) in water and 0.01 ng/g (7β-spironolactone and canrenone) to 1.44 ng/g (aldosterone) in sediment in dry weight. Natural aldosterone was the predominant mineralocorticoid detected in both water and sediment samples, indicating the mineralocorticoid pollution in Taihu Lake was mainly derived from human and/or animal excrement rather than pharmaceutical industry and usage. Two metabolites 7β-spironolactone and 7α-thio spironolactone were first found in this study. Low ratios of metabolites to spironolactone were observed in sediment (0.05–0.75) in contrast to water (0.12–2.26), indicating that spironolactone was prone to degradation in water phase compared to sediment environment.
Lack of Association Between Polymorphisms in TXNRD2 and LMX1B and Primary Open-Angle Glaucoma in a Saudi Cohort
Authors: Kondkar Altaf A. , Azad Taif A. , Alobaidan Abdullah S. , Sultan Tahira , Osman Essam A. , Almobarak Faisal A. , Lobo Glenn P. , Al-Obeidan Saleh A. 
Published: August 2021

Objective: Recent studies have demonstrated an association of single nucleotide polymorphisms (SNPs) rs35934224 in TXNRD2 and rs6478746 near LMX1B genes in primary open-angle glaucoma (POAG) among Europeans. We performed a retrospective, case-control study to investigate the association between the rs35934224 (TXNRD2) and rs6478746 (LMX1B) and POAG in a middle-eastern population from Saudi Arabia.Methods: DNA from 399 participants consisting of 150 POAG cases (83 males and 67 females) and 249 controls (135 males and 114 females) were genotyped using TaqMan® real-time PCR. Statistical tests were performed to evaluate genetic association with POAG and related clinical indices.Results: The minor allele frequency (MAF) of rs35934224[T] was 0.19 and 0.20 in POAG and controls, respectively. The difference was non-significant (odds ratio [OR] = 1.08, 95% confidence interval [CI] = 0.75–1.55, p = 0.663). Likewise, rs6478746[G] MAF was 0.12 in both cases and controls with no statistical significance (OR = 1.02, 95% CI = 0.67–1.56, p = 0.910). Genotype analysis showed no association with POAG for both the SNPs in combined and gender-stratified groups. Regression analysis showed no significant effect of risk factors such as age, sex, rs35934224, and rs6478746 genotypes on POAG outcome. Furthermore, both the SNPs showed no significant genotype effect on clinical indices such as intraocular pressure (IOP) and cup/disc ratio in POAG patients.Conclusions: Rs35934224 in TXNRD2 and rs6478746 near LMX1B genes are not associated with POAG or related clinical indices such as IOP and cup/disc ratio in a Saudi cohort. Since the study is limited by sample size further investigations are needed to confirm these results in a larger cohort.
Cox-sMBPLS: An Algorithm for Disease Survival Prediction and Multi-Omics Module Discovery Incorporating Cis-Regulatory Quantitative Effects
Authors: Vahabi Nasim , McDonough Caitrin W. , Desai Ankit A. , Cavallari Larisa H. , Duarte Julio D. , Michailidis George 
Published: August 2021

BackgroundThe development of high-throughput techniques has enabled profiling a large number of biomolecules across a number of molecular compartments. The challenge then becomes to integrate such multimodal Omics data to gain insights into biological processes and disease onset and progression mechanisms. Further, given the high dimensionality of such data, incorporating prior biological information on interactions between molecular compartments when developing statistical models for data integration is beneficial, especially in settings involving a small number of samples.ResultsWe develop a supervised model for time to event data (e.g., death, biochemical recurrence) that simultaneously accounts for redundant information within Omics profiles and leverages prior biological associations between them through a multi-block PLS framework. The interactions between data from different molecular compartments (e.g., epigenome, transcriptome, methylome, etc.) were captured by usingcis-regulatory quantitative effects in the proposed model. The model, coined Cox-sMBPLS, exhibits superior prediction performance and improved feature selection based on both simulation studies and analysis of data from heart failure patients.ConclusionThe proposed supervised Cox-sMBPLS model can effectively incorporate prior biological information in the survival prediction system, leading to improved prediction performance and feature selection. It also enables the identification of multi-Omics modules of biomolecules that impact the patients’ survival probability and also provides insights into potential relevant risk factors that merit further investigation.
Screening of DNA Damage Repair Genes Involved in the Prognosis of Triple-Negative Breast Cancer Patients Based on Bioinformatics
Authors: Wang Nan , Gu Yuanting , Chi Jiangrui , Liu Xinwei , Xiong Youyi , Zhong Chaochao , Wang Fang , Wang Xinxing , Li Lin 
Published: August 2021

Background: Triple-negative breast cancer (TNBC) is a special subtype of breast cancer with poor prognosis. DNA damage response (DDR) is one of the hallmarks of this cancer. However, the association of DDR genes with the prognosis of TNBC is still unclear.Methods: We identified differentially expressed genes (DEGs) between normal and TNBC samples from The Cancer Genome Atlas (TCGA). DDR genes were obtained from the Molecular Signatures Database through six DDR gene sets. After the expression of six differential genes were verified by quantitative real-time polymerase chain reaction (qRT-PCR), we then overlapped the DEGs with DDR genes. Based on univariate and LASSO Cox regression analyses, a prognostic model was constructed to predict overall survival (OS). Kaplan–Meier analysis and receiver operating characteristic curve were used to assess the performance of the prognostic model. Cox regression analysis was applied to identify independent prognostic factors in TNBC. The Human Protein Atlas was used to study the immunohistochemical data of six DEGs. The prognostic model was validated using an independent dataset. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes analysis were performed by using gene set enrichment analysis (GSEA). Single-sample gene set enrichment analysis was employed to estimate immune cells related to this prognostic model. Finally, we constructed a transcriptional factor (TF) network and a competing endogenous RNA regulatory network.Results: Twenty-three differentially expressed DDR genes were detected between TNBC and normal samples. The six-gene prognostic model we developed was shown to be related to OS in TNBC using univariate and LASSO Cox regression analyses. All the six DEGs were identified as significantly up-regulated in the tumor samples compared to the normal samples in qRT-PCR. The GSEA analysis indicated that the genes in the high-risk group were mainly correlated with leukocyte migration, cytokine interaction, oxidative phosphorylation, autoimmune diseases, and coagulation cascade. The mutation data revealed the mutated genes were different. The gene-TF regulatory network showed that Replication Factor C subunit 4 occupied the dominant position.Conclusion: We identified six gene markers related to DDR, which can predict prognosis and serve as an independent biomarker for TNBC patients.