bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salivary Metabolic Hormones as Biomarkers of Childhood Obesity
Authors: Manohar Shiny Lizia , Janardhanam Hemamalini Arambakkam , Ravichandran Latha 
Published: October 2021

Abstract
IntroductionObesity is a major threat to the health and development of children. While hormones in serum samples have been extensively investigated in childhood obesity research, our study attempts to explore salivary metabolic hormones as biomarkers of childhood obesity.MethodsObese (n=83) and non-obese (n=83) school children aged 6-11 years were recruited as cases and controls, respectively, for this case-control study. Salivary concentrations of the hormones, Ghrelin, Leptin, Adiponectin and Insulin were assessed and compared between obese and non-obese children to evaluate their role in childhood obesity. Results The mean ghrelin (1.69 ± 0.38 pg/ml) and adiponectin (95.53 ± 64.92 ng/ml) levels of the non-obese children were higher than the mean values of the obese, while the mean values of the hormones leptin (265.95 ± 83.16 pg/ml) and insulin (224.56 ± 184.86 pg/ml) were found to be higher among the obese. Higher levels of the hormones insulin (OR: 1.09; 95% CI: 1.05-1.12) and leptin (OR: 1.03; 95% CI: 1.02-1.04) posed higher risks for childhood obesity, followed by adiponectin, which was found to be lower among the cases (OR: 0.99; 95% CI: 0.99995-0.99998) which increased the risk of childhood obesity.ConclusionThis study demonstrates the efficacy of saliva as a promising non-invasive tool in childhood obesity research and highlights the significance of appetite regulatory hormones as biomarkers of childhood obesity.
Is the Status of CXCL9-CXCL10-CXCL11/CXCR3 axes Altered in Brucellosis Patients?
Authors: Hassanshahi Farzaneh Hassanshahi , Hassanshahi Gholamhossein , karimabad Mojgan Noroozi 
Published: October 2021

Abstract
Aim and objectives: Brucellosis is common in most parts of the world between humans and domestic animals. The causative agent is bacteria from the Brucella family, which are intracellular pathogens. Despite, Brucella spp. Is able to infect humans, their ability to induce production of chemokines by lung cells is unknown. In this study, we showed an association between brucellosis and CXCL9, 10 and 11chemokinesMethods:We have totally recruited 71 brucella patients who have referred to the either local health centers or hospitals of kerman province. Diagnosis of acute brucellosis was made based on a brief history of the disease,clinical examinations results of serological tests,antibody titration in serums was performed by bacterial wright method, agglutination and 2ME with antigen. Brucellosis patients CXCL9, CXCL10 and CXCL11 serum levels were detected by sandwich ELISA using paired chemokine-specific mAbs, according to the manufacturer’s guidelines. Transcription levels of mRNA for the CXCR3 as the chemokine receptor for CXCL9, CXCL10 and CXCL11 was examined by QRT-PCR using primers. Results: We have found that there was a positive correlation between the level of chemokines and their receptor. Here ELISA test showed that B. abortus induces expression of some IFN-γ induced CXCL9, 10 and 11chemokines in brucellosis patients. We have also showed the increased expression of CXCR3 as their shared receptors in brucellosis patients with real time-PCR. Conclusion: According to these findings there was a positive correlation between the level of chemokines and their receptor. We have found that there was a positive correlation between the level of chemokines and their receptor.
Whole exome sequencing identified novel mutation in SUOX gene causes extremely rare autosomal recessive isolated sulfite oxidase deficiency
Authors: Zhang Rui , Hao Yajing , Xu Ying , Qin Jiale , Wang Yanfang , Dey Subrata Kumar , Li Chen , Wang Huilin , Banerjee Santasree 
Published: October 2021

Abstract
BackgroundIsolated sulfite oxidase deficiency (ISOD) is the rarest types of life-threatening neurometabolic disorders characterized by neonatal intractable seizures and severe developmental delay with an autosomal recessive mode of inheritance. ISOD is extremely rare and only 29 mutations have been identified and reported worldwide. Germline mutation in SUOX gene causes ISOD. MethodsHere, we investigated a 5-days old Chinese girl, presented with intermittent tremor or seizures of limbs, neonatal encephalopathy, subarachnoid cyst and haemorrhage, dysplasia of corpus callosum, neonatal convulsion, respiratory failure, cardiac failure, hyperlactatemia, severe metabolic acidosis, hyperglycemia, hyperkalemia, moderate anemia, atrioventricular block and complete right bundle branch block. Karyotype and chromosomal microarray analysis were performed and identified no chromosomal abnormalities in the proband. Whole exome sequencing was performed for the proband. Sanger sequencing was performed for the proband and her family members. ResultsWhole exome sequencing identified a novel heterozygous deletion (c.1406_1421delCCTGGCAGGTGGCTAA) and a previously reported heterozygous substitution (c.1200C>G) in exon 6 of the SUOX gene in the proband. The novel heterozygous deletion leads to frameshift (p.Thr469Serfs*20) which results into the formation of a truncated sulfite oxidase of 488 amino acids. The substitution leads to a premature stop codon (p.Tyr400*) followed by the formation of a truncated sulfite oxidase of 399 amino acids. Hence, both the variants are loss-of-function variants. The proband’s father and mother is carrying the substitution and deletion in a heterozygous state respectively. These two variants were not identified in the elder brother of the proband as well as in the 100 healthy individuals. ConclusionHere, we reported the first variant of SUOX gene associated ISOD in Chinese population. Our study not only expand the mutational spectrum of SUOX gene associated ISOD, but also strongly suggested the application of whole exome sequencing for identifying the novel disease-causing mutation in candidate genes.
Utilization of Long Acting And Permanent Contraceptive Method and Associated Factor Among Women of Reproductive Age in West Guji Zone, Southern Ethiopia, 2018.
Authors: Zeleke Eden Dagnachew , Assefa Dawit Getachew , Woldesenbet Tigist Tekle , Gido Rediet 
Published: October 2021

Abstract
Background: Increasing access to family planning helps to ensure the reproductive right, decrease unintended pregnancy, improve the health and nutritional status of children, reduction of maternal mortality, and enhance longer birth spacing . There is continually low utilization of long-acting and permanent contraceptive methods among low and middle-income countries. The aim of this study was to assess the Utilization of Long-Acting and permanent Contraceptive methods and associated factors among Women of Reproductive Age in the West Guji Zone.Methods: Hospital-based cross-sectional design was conducted among women of reproductive age in the West Guji Zone. A systematic random sampling method was used to select study subjects for the survey with a sample size of 507. Descriptive analysis was utilized to summarize the data while logistic regression to assess factors influencing the utilization of Long-Acting and permanent Contraceptive methods use. Statistical significance was declared for variables outcomes of the P-value less than 0.05.Result: Current utilization of Long-Acting And permanent Contraceptive methods at West Guji zone among the reproductive-aged group was 51.1%. More than the median of participants had negative altitude (72.4%) and poor knowledge (57%) towards the long-acting and permanent contraceptive methods. Educational status of women, the number of alive children, acceptance of utilization of Long-Acting and permanent Contraceptive methods, how treated by other staff, and waiting time during service delivery are significant determinant factors of long-acting and permanent family planning methods. Conclusion: Educational status, number of alive children ( Parity) , acceptance of Long-Acting And permanent Contraceptive methods , how treated by other staff, and waiting time to get the service are statistically significant predictors of utilization of long-acting and permanent family planning methods. More than half of women had a negative attitude and poor knowledge of Long-Acting and permanent Contraceptive methods.
3C Based DNA Hybridization Method for Chromosomal Translocation Screening
Authors: Absalan Moloud , Jabbarpour Zahra , Ghahremani Mohammad Hossein , Motevaseli Elaheh , Mahmoudian Fatemeh , Karimi Roya , Tavoosidana Gholamreza 
Published: October 2021

Abstract
Background: DNA probes have been widely used as diagnostic tools for chromosomal translocations in malignancies. PCR-based methods often fail to detect translocations such as MYC/TRD in chronic lymphocytic leukemia. In addition, microscopic techniques cannot be helpful due to size detection limitations. This study sought to design a screening tool using immobilized ssDNA probes on a nitrocellulose membrane followed by 3C library fragments hybridization. Results: Hence, we focused on developing a suitable 27 bp specific probe for the juxtaposed region of MYC and TRD. Colloidal gold nanoparticles (AuNP) functionalized translocation fragments of the MYC gene with a thiol group (MYC-AuNP-probe). Then TRD-probes were immobilized on nitrocellulose surface to detect TRD/MYC translocation in the SKW3 cells. Hybridization between DNA probes and 3C-library fragments of SKW3 cells was determined by color intensity. Optimal hybridization of the 3C library sample of the cell line to TRD-probe and MYC-AuNP-probe showed higher color intensity due to their convenient proximity to the juxtaposed region compared with normal cells. Conclusions: Our results demonstrated that DNA hybridization colorimetric assay could be a helpful technique in chromosomal rearrangements screening. Accordingly, the combination of 3C based techniques and DNA-DNA hybridization can identify cancer cells with high specificity and sensitivity.
Metabolic Differences Between Suaeda Salsa and Puccinellia Tenuiflora Under Saline-alkali Stress
Authors: Chen Qi , Xie Huansong , Wei Guanyun , Guo Xiaorui , Zhang Jian , Lu Xueyan , Tang Zhonghua 
Published: October 2021

Abstract
Background: Salinization of soil is an urgent problem that restricts agroforestry production and environment protection. Substantial accumulation of metal ion or high alkaline alters plant metabolites and may even cause plant death. In order to explore the differences in the response strategies between Suaeda salsa (S. salsa) and Puccinellia tenuiflora (P. tenuiflora), two main constructive species that survive in saline-alkali soil, their metabolic differences were characterized.Result: Metabolomics was conducted to study the role of metabolic differences between S. salsa and P. tenuiflora under saline-alkali stress. A total of 68 significantly different metabolites were identified by GC-MS, including 9 sugars, 13 amino acids, 8 alcohols, and 34 acids. A more detailed analysis indicated that P. tenuiflora utilizes sugars more effectively and may be salt-alkali tolerant via sugar consumption while S. salsa mainly utilizes amino acids, alcohols, and acids to resist salt-alkali stress. Measurement of phenolic compounds showed that more C6C3C6-compounds were accumulated in P. tenuiflora while more C6C1-compounds, phenolic compounds that can be used to defense stress as signaling molecules, were accumulated in S. salsa.Conclusion: Our observations suggest that S. salsa resists the toxicity of saline-alkali stress using aboveground organs and P. tenuiflora eliminates the poison of saline-alkali via roots. S. salsa has a stronger ability of habitat transformation and can provide better habitat for other plants.
Effect of Blood Pressure on Cardiovascular Outcomes: A 15-Year Prospective Cohort Study
Authors: Yu Qi , Jin Qing-Dong 
Published: October 2021

Abstract
Objective

The association between blood pressure(BP) and cardiovascular outcomes has not been well investigated by large prospective studies on Chinese. We aim to analyze the association of BP with cardiovascular outcomes in Chinese population.
Method

We included a total of 4,569 adults aged 40–90 years from the China Health and Nutrition Survey (CHNS) cohort. Cox proportional hazards regression models were used to estimate hazard ratios and 95% CIs. Restricted cubic spline analyses were used to explore linear and nonlinear relationships of BP with cardiovascular outcomes.
Result

With a mean follow-up of 12.1 years, a total of 4,569 individuals were enrolled in our study, of whom 403 developed cardiovascular outcomes. Multivariable adjusted Cox models showed a strong positive association between BP and cardiovascular outcomes. SBP was significantly associated with composite outcome(HR per 10 mmHg 1.23[1.16–1.29]), myocardial infarction(MI)(HR per 10 mmHg 1.17[1.07–1.27]), and stroke(HR per 10 mmHg 1.29[1.21–1.38]). DBP was significantly associated with composite outcome(HR per 10 mmHg 1.32[1.20–1.44]), MI(HR per 10 mmHg 1.26[1.10–1.44]), and stroke(HR per 10 mmHg 1.39[1.25–1.55]). Restricted cubic spline analyses showed linear relationships of either SBP or DBP with composite outcome, MI and stroke.
Conclusion

Either SBP or DBP is independently and linearly related to the risk of cardiovascular outcomes. These associations are steeper for stroke than for MI, and vary widely by age, use of antihypertensive treatment, and diabetes status.
Treatment of Severe Refractory Thrombocytopenia in Brucellosis With Eltrombopag
Authors: Zhang Xianwen , Ren Peng , Sang Baohua , Song Chunyan , Lin Yunbi , Lei Qingling , Lv Yu , Yang Chunhui , Li Na , Tian Xin , Huang Tilong 
Published: October 2021

Abstract
Background: Brucellosis is a common zoonotic illness in the world. Brucellosis is often characterized by hematologic abnormalities, including mild anemia, leukopenia, thrombocytopenia, or pancytopenia. However, severe refractory thrombocytopenia in brucellosis is very rare and easily misdiagnosed.Case presentation: The patient was a 5-year-old girl with brucellosis who developed severe refractory thrombocytopenia (platelet count: 3×109/L) with complaints of epistaxis, skin petechiae and purpura. Most conventional treatments including glucocorticoids and intravenous immunoglobulin (IVIg) did not elevate her platelets, but eltrombopag worked well and her platelet count recovered rapidly. One week later, the patient's symptoms improved and the platelet count returned to normal.Conclusions:Patients with severe refractory thrombocytopenia, particularly resistant to IVIg and steroid treatment should be considered for second-line drugs such as eltrombopag. Our results also increase the application experience of eltrombopag in Chinese patients with severe refractory thrombocytopenia in brucellosis. To the best of our knowledge, this case is the first reported case of the successful treatment of severe refractory thrombocytopenia in brucellosis with eltrombopag.
Association Between Single-Nucleotide Polymorphisms of NKX2.5 and Congenital Heart Disease in Chinese Population: A Meta-Analysis
Authors: Chen Huan , Li Tianjiao , Wu Yuqing , Wang Xi , Wang Mingyuan , Wang Xin , Fang Xiaoling 
Published: October 2021

Abstract
Background: NKX2.5 is a transcription factor that plays a key role in cardiovascular growth and development. Many independent studies have been conducted to investigate the association between the single nucleotide polymorphism 606G>C (rs3729753) in the coding region of NKX2.5 and congenital heart disease (CHD), although the results were inconsistent. This study aimed to reveal as much as possible the relationship between NKX2.5 single nucleotide polymorphism 606G>C and the risk of congenital heart disease in the Chinese population through meta-analysis.Methods and Results: After retrieving related articles in PubMed, MEDLINE, EMBASE, Web of science, Coherane, China National Knowledge Infrastructure (CNKI), Wanfang DATA, VIP database until Aug 2021, a total of 8 studies were finally included. Then, we merged the qualified research data into allele model, dominant model, recessive model, heterozygous model, homozygous model, additive model respectively. Overall meta-analysis results showed that 606G>C was not associated with congenital heart disease of the Chinese population in any model. Also, subgroup analysis based on congenital heart disease type gave the same negative result. Sensitivity analysis showed that there was no significant correlation after the deletion of each study. The results were negative and the heterogeneity was not significant.Conclusion: Our results show that NKX2-5 single nucleotide polymorphism 606G> C may not lead to the risk of congenital heart disease in Chinese.
Study of effect modifiers of genetically predicted CETP reduction
Authors: Legault Marc-André , Barhdadi Amina , Gamache Isabel , Lemaçon Audrey , Perreault Louis-Philippe Lemieux , Grenier Jean-Christophe , Sylvestre Marie-Pierre , Hussin Julie , Rhainds David , Tardif Jean-Claude , Dubé Marie-Pierre 
Published: October 2021

Abstract
Genetic variants in drug targets can be used to predict the effect of drugs. Here, we extend this principle to assess how sex and body mass index may modify the effect of a genetically predicted lower CETP levels on biomarkers and cardiovascular outcomes. We found sex and BMI to be modifiers of the association between genetically predicted lower CETP and lipid biomarkers in UK Biobank participants. Female sex and lower BMI were associated with higher HDL-cholesterol and lower LDL-cholesterol for a same genetically predicted reduction in CETP concentration. We found that sex also modulated the effect of genetically lower CETP on cholesterol efflux capacity in samples from the Montreal Heart Institute Biobank. However, these modifying effects did not extend to sex-differences in cardiovascular outcomes in our data. Our results provide insight on the clinical effects of CETP inhibitors in the presence of effect modification based on observational genetic data. The approach can support precision medicine applications and help assess the external validity of clinical trials.
Chronic overexpression of neuropeptide Y in the skin is sufficient to induce inflammation and epidermal and dermal pathology
Authors: Anderson Zoya T. , Palmer Joseph W. , Slominski Andrzej T. , Proctor Jennifer L. , Idris Misgana I. , Wang Dezhi , Martin Adam E. , Harris Melissa L. 
Published: October 2021

Abstract
Neuropeptide Y (NPY) is a pleiotropic peptide produced in the central nervous system and peripheral organs. Despite conjectures that NPY may have a role in skin physiology and pathology, the effects of NPY in this organ remain poorly understood. We reported that a knock-in mouse with entopic NPY overexpression exhibits significantly elevated NPY in the skin, accompanied by premature and progressive hair graying secondary to depletion of melanocyte stem cells within hair follicles. However, the question remains as to whether NPY overexpression in the skin can induce non-melanocyte pathology. In this study, we employed this mouse to investigate the consequences of skin-specific overexpression of NPY. Our findings show that chronic NPY overexpression in the skin induces dermal fibrosis and epidermal hyperkeratosis. Additionally, NPY overexpression induces significant accumulation of macrophages and regulatory T cells in the dermis. RNA sequencing of whole skin from NPY-overexpressing mice further reveals NPY-mediated transcriptional changes consistent with inflammatory processes and inflammation-associated skin changes and highlights novel cell types involved in the NPY-mediated response in the skin. Together, these results provide long-awaited evidence of NPY’s involvement in skin pathology, providing a background for defining the precise role of NPY in the regulation of cutaneous homeostasis and disease.
Using Peroxymonosulfate-Ozone Advanced Oxidation For The Treated Wastewater Disinfection and Amoxicillin Micro-Pollutant Removal Simultaneously
Authors: Gholikandi Gagik Badalians , Mollazadeh Atefeh , Farimaniraad Hamidreza , Masihi Hamidreza 
Published: October 2021

Abstract
Due to the recent efforts to improve the conventional disinfection methods efficiency of wastewater treatment plants effluent, in this study, the efficiency of the peroxymonosulfate-ozone (PMS+O3) advanced oxidation process in lab scale by the aim of disinfection and simultaneous removal of existing amoxicillin micro-pollutant under optimum operational condition was investigated for the first time. Furthermore, the results were compared with those obtained from the experiments conducted employing persulfate-ozone (PS+O3), hydrogen peroxide-ozone (H2O2+O3), and ozonation (O3) processes. For this purpose, the main parameters including the total coliforms, amoxicillin concentration, turbidity, chemical oxygen demand (COD), biochemical oxygen demand (BOD5), total nitrogen (TN), electrical conductivity (EC), total dissolved solids (TDS), and total suspended solids (TSS) were considered. The test results show that under optimized operational conditions (retention time of 20 minutes, ozone dosage rate of 0.83 mmol/L, and peroxymonosulfate concentration of 0.06 mmol , 99.99% total coliforms (e.g., the number of total coliforms reached consistently less than 400 MPN in 100 ml) removal was reached by peroxymonosulfate-ozone advanced oxidation process. Also, amoxicillin concentration removal efficiency reached 90±2%. In comparison, although the total coliforms reduction of PS+O3 and H2O2+O3 methods in 30 min are approximately the same, the amoxicillin concentration removal efficiency is about 60-70%. Due to the importance of ensuring effluent quality, the related removal efficiency of other considered parameters is also evaluated and presented. Eventually, the peroxymonosulfate-ozone method can be considered as a novel efficient approach for wastewater plants effluent disinfection and amoxicillin micro-pollutant removal simultaneously which is a novel approach.
Single-cell Profiling of Immune cells After Neoadjuvant Pembrolizumab and Chemotherapy in ⅢA non-small cell lung cancer (NSCLC)
Authors: Ren Xiubao , Hui Zhenzhen , Zhang Jiali , Ren Yulin , LI XIAOLING , Yan Cihui , Yang Lili , Yu Wenwen , Wang Tao , Xiao Shanshan , Chen Yulong , Zhang Ran , Zhang Lianmin , Xu Feng , Wei Feng , You Jian 
Published: October 2021

Abstract
The combination of immune checkpoint inhibitors (ICIs) with chemotherapy (chemoimmunotherapy) in the neoadjuvant setting have achieved favorable clinical benefits in non-small cell lung cancer (NSCLC), but the mechanism of clinical responses remain unclear. To identify factors associated with the clinical outcome, we provide a rich resource of 186,477 individual immune cells from matched multiple immune-relevant tissue sites and peripheral blood of four treatment-naïve and eight neoadjuvant chemoimmunotherapy treated ⅢA NSCLC patients (responders versus non-responders) by single-cell RNA-seq and TCR-seq. We showed that the synergistic increase of B cells and CD4+ T cells are associated with positive therapeutic response of neoadjuvant chemoimmunotherapy. B cell IgG subclasses IgG1 and IgG3 play a critical role in anti-tumor immune response in tumor lesion, and this process was driven by increased IL-21 protein secreted by infiltrated T follicular helper (Tfh) cells after neoadjuvant chemoimmunotherapy. Furthermore, we uncovered several critical events for positive clinical outcome, including the diminished activated TNFRSF4+ regulatory T cells (Tregs), increased LAMP3+ dendritic cells (DCs), high pre-therapy peripheral blood T-cell diversity, and the expansion of intratumoral CD4+ T clones and peripheral CD8+ T clones. In total, our comprehensive study of the single-cell profile of immune cells provide mechanistic insight of clinical responses and identified novel predictive factors and potential therapeutic targets for improving the efficiency of neoadjuvant chemoimmunotherapy in NSCLC.