bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toxicity of Atrazine to Marine Invertebrates Under Flow-Through Conditions—Eastern Oyster (Crassostrea virginica) and Mysid Shrimp (Americamysis bahia)
Authors: Brain R. A. , Anderson J. C. , Hanson M. L. 
Published: April 2021

AbstractThe triazine herbicide atrazine is routinely detected in freshwaters, and has also been detected in coastal waters in Europe and the US. Relatively few atrazine studies have been conducted with estuarine/marine invertebrates. This study sought to contribute additional invertebrate atrazine toxicity data using model species, Eastern oyster (Crassostrea virginica) and Mysid shrimp (Americamysis bahia). Specifically, we investigated (1) acute effects on survival and growth of Eastern oyster, and (2) acute and chronic effects on survival, reproduction, and growth of the Mysid shrimp. No oyster mortality was observed following 96-h exposure to up to 17 mg a.i./L, but shell growth was reduced by 0.55% to 16% in 1.0, 9.2, and 17 mg a.i./L treatments, resulting in a 96-h EC50 of > 17 mg a.i./L. In the 96-h Mysid test, mortality rates of 5 to 70% were observed in 1.7, 2.4, 3.6, and 6.4 mg a.i./L treatments, and the 96-h LC50 was 5.4 mg a.i./L. Sub-lethal effects were observed among surviving Mysids exposed to ≥ 2.4 mg a.i./L. After 28 days of exposure to up to 1.1 mg a.i./L, there were no effects on survival or reproduction. The LOEC was 0.50 mg a.i./L, based on reduced body size, and the NOEC was 0.26 mg a.i./L. Overall, the results indicate that atrazine is slightly toxic towards Eastern oyster and moderately toxic to the Mysid shrimp under acute exposure conditions. These data will help to fill a gap in the literature and inform risk assessment of potential effects of atrazine towards estuarine/marine communities.
Prediction of an oxygen extraction fraction map by convolutional neural network: validation of input data among MR and PET images
Authors: Matsubara Keisuke , Ibaraki Masanobu , Shinohara Yuki , Takahashi Noriyuki , Toyoshima Hideto , Kinoshita Toshibumi 
Published: April 2021

Abstract
Purpose
Oxygen extraction fraction (OEF) is a biomarker for the viability of brain tissue in ischemic stroke. However, acquisition of the OEF map using positron emission tomography (PET) with oxygen-15 gas is uncomfortable for patients because of the long fixation time, invasive arterial sampling, and radiation exposure. We aimed to predict the OEF map from magnetic resonance (MR) and PET images using a deep convolutional neural network (CNN) and to demonstrate which PET and MR images are optimal as inputs for the prediction of OEF maps.

Methods
Cerebral blood flow at rest (CBF) and during stress (sCBF), cerebral blood volume (CBV) maps acquired from oxygen-15 PET, and routine MR images (T1-, T2-, and T2*-weighted images) for 113 patients with steno-occlusive disease were learned with U-Net. MR and PET images acquired from the other 25 patients were used as test data. We compared the predicted OEF maps and intraclass correlation (ICC) with the real OEF values among combinations of MRI, CBF, CBV, and sCBF.

Results
Among the combinations of input images, OEF maps predicted by the model learned with MRI, CBF, CBV, and sCBF maps were the most similar to the real OEF maps (ICC: 0.597 ± 0.082). However, the contrast of predicted OEF maps was lower than that of real OEF maps.

Conclusion
These results suggest that the deep CNN learned useful features from CBF, sCBF, CBV, and MR images and predict qualitatively realistic OEF maps. These findings suggest that the deep CNN model can shorten the fixation time for 15O PET by skipping 15O2 scans. Further training with a larger data set is required to predict accurate OEF maps quantitatively.
Building up an ecologically sustainable and socially desirable post-COVID-19 future
Authors: Duflot Rémi , Baumeister Stefan , Burgas Daniel , Eyvindson Kyle , Triviño María , Blattert Clemens , Kuparinen Anna , Potterf Mária 
Published: April 2021

AbstractCOVID-19 crisis has emphasized how poorly prepared humanity is to cope with global disasters. However, this crisis also offers a unique opportunity to move towards a more sustainable and equitable future. Here, we identify the underlying environmental, social, and economic chronic causes of the COVID-19 crisis. We argue in favour of a holistic view to initiate a socio-economic transition to improve the prospects for global sustainability and human well-being. Alternative approaches to “Business-As-Usual” for guiding the transition are already available for implementation. Yet, to ensure a successful and just transition, we need to change our priorities towards environmental integrity and well-being. This necessarily means environmental justice, a different worldview and a closer relationship with nature.
Influence of Stress States on Forming Hybrid Parts with Sheet Metal and Additively Manufactured Element
Authors: Hafenecker Jan , Papke Thomas , Merklein Marion 
Published: April 2021

AbstractHybrid parts with additively manufactured elements (AME) combine the advantages of two or more manufacturing processes, e.g., forming and additive manufacturing (AM), and thus offer a solution to the increasing demands of industrial trends such as personalized mass production. Despite their advantageous properties, research in this field still lacks in clear classification and process interactions. Due to the strong influence of the AME on the formability of hybrid parts, the combination of laser-based powder bed fusion (PBF-LB) with subsequent sheet metal forming is examined in this paper. Therefore, cylindrical functional elements are built up on sheet metal and the resulting hybrid components are subsequently formed. Common forming processes such as bending, stretch forming and deep drawing are compared in regard to the different stress states. The results show a reduction in formability for hybrid components compared to conventional sheet metal materials. Reasons found are geometrical properties, gradients of mechanical properties and induced stresses. Consequently, requirements for the additive manufacturing process regarding a subsequent forming process are outlined. Namely, the gradient of mechanical properties should be smoothened, residual stresses kept low and the design of AMEs should avoid stress concentration.
Iron Deficiency – Not Only a Premenopausal Topic After Bariatric Surgery?
Authors: Kunst Ines , Krebs Michael , Dreschl Bettina , Prager Gerhard , Meyer Elias , Kautzky-Willer Alexandra , Ranzenberger-Haider Tamara 
Published: April 2021

Abstract
Purpose
In our centre, specialized high dose multivitamin supplementation designed to meet the needs of patients after gastric bypass surgery is routinely recommended in the early postoperative period. The aim of the present study was to analyse whether iron supplementation prescribed in clinical practice is sufficient in both sexes and whether multivitamin supplementation standardized for women might potentially lead to iron overload in men.

Materials/Methods
This was a retrospective study covering the period up to 36 months after bariatric surgery. Three groups were compared (men, premenopausal and postmenopausal women). The iron status was evaluated employing serum ferritin concentrations.

Results
A total of 283 patients who had at least one follow-up visit between January 2015 and April 2018 at a specialized academic outpatient centre were included (71 men, 130 premenopausal women, 82 postmenopausal women). Thirty-six months after surgery, 33.3%, 68.4% and 54.5% of the men, pre- and postmenopausal women, respectively, were iron deficient. The preoperative prevalence of excess ferritin levels was 13.7% in premenopausal, 3.0% in postmenopausal women, 5.7% in men and declined in the following months.

Conclusion
Iron deficiency is very common after gastric bypass surgery, and even high dosages of multivitamin and mineral supplements might not be sufficient to prevent the development of iron deficiency. Men, pre- and postmenopausal women differ in their prevalence of iron deficiency which demands adapted iron dosage regimens based on the sex and the age. Iron overload is rare in all observed groups and highest in premenopausal women.

Graphical abstract
Outcomes of robotically assisted laparoscopic lateral suspension (RALLS) with mesh for anterior and apical prolapse
Authors: Dällenbach Patrick , Alec Milena , Boulvain Michel , Shabanov Stas 
Published: April 2021

AbstractAbdominal sacral colpopexy/hysteropexy is the gold standard for the treatment of vaginal apex support. However, dissection of the promontory may expose to potentially life-threatening complications. To avoid this risk, laparoscopic lateral suspension with mesh is an alternative. Robotic assistance may be helpful in both techniques. The objective of our study was to evaluate outcomes of robotically assisted laparoscopic lateral suspension (RALLS) with mesh for anterior and apical pelvic organ prolapse (POP). From March 2012 to January 2018, 59 consecutive patients underwent RALLS using titanized polypropylene mesh. Between August 2017 and September 2019, all patients were contacted to assess outcome. We performed a clinical exam and asked them to complete the patient global impression of improvement (PGI-I) questionnaire. Fifty-four patients (91.5%) were available for follow-up. Mean age was 58.5 years (28.8–79.8). There were no perioperative complications. The mean follow-up was 33.6 months (11.2–74.1).The objective cure rate (no prolapse beyond hymen) and the subjective cure rate (PGI-I ≤ 2) were 83.3% and 77.2%, respectively. Five women (9.3%) were reoperated for POP recurrence. There was no erosion. Of the 20 women complaining of stress urinary incontinence (SUI) preoperatively, 12 (60%) were cured without any additional SUI procedure. Only two women (10%) required TVT for persistent grade 2 SUI. Two women (5.9%) developed de novo SUI, but none of them required an operation. RALLS repair for POP with mesh is safe and effective and may represent an alternative to sacral colpopexy/hysteropexy.
Rare germline variants in DNA repair-related genes are accountable for papillary thyroid cancer susceptibility
Authors: Mio Catia , Verrienti Antonella , Pecce Valeria , Sponziello Marialuisa , Damante Giuseppe 
Published: April 2021

Abstract
Background
Understanding the molecular mechanisms underlying papillary thyroid cancer (PTC) proved to be vital not only for diagnostic purposes but also for tailored treatments. Despite the strong evidence of heritability, only a small subset of alterations has been implicated in PTC pathogenesis. To this reason, we used targeted next-generation sequencing (NGS) to identify candidate variants implicated in PTC pathogenesis, progression, and invasiveness.

Methods
A total of 42 primary PTC tissues were investigated using a targeted next-generation sequencing (NGS) panel enlisting 47 genes involved in DNA repair and tumor progression.

Results
We identified 57 point mutations in 78.5% of samples (n = 32). Thirty-two somatic mutations were identified exclusively in known thyroid cancer genes (BRAF, KRAS, NRAS, and TERT). Unpredictably, 45% of the all identified mutations (n = 25) resulted to be germline, most affecting DNA repair genes. Interestingly, none of the latter variants was in the main population databases. Following ACMG classification, 20% of pathogenic/likely pathogenic and 68% of variant of unknown significance were identified.

Conclusions
Overall, our results support the hypothesis that rare germline variants in DNA repair genes are accountable for PTC susceptibility. More data, including the segregation analysis in affected families, should be collected before definitely annotate these alterations and to establish their potential prognostic and treatment implications.
Chemosensory dysfunction in Cushing’s syndrome
Authors: Heger Elena , Rubinstein German , Braun Leah T. , Zopp Stephanie , Honegger Jürgen , Seidensticker Max , Reincke Martin , Oßwald Andrea 
Published: April 2021

AbstractPurposeCushing’s syndrome (CS) can lead to structural changes in the brain and cognitive impairment, but chemosensory function has not been investigated yet. The aim was to analyze sense of smell and taste in patients with CS and explore the effect of therapy.MethodsThe study cohort comprised 20 patients with florid CS treated between 2018 and 2020 in the outpatient clinic of the LMU Munich. We compared these 20 patients with CS to 40 healthy subjects matched for age, sex, and smoking status. Patients’ sense of smell and taste was examined at diagnosis and 3 months after successful therapeutic surgery leading to clinical and biochemical remission. Odor threshold, discrimination, and identification were measured with “Sniffin’ Sticks”, taste was measured with “Taste Strips”. Perceived sense of smell and taste was retrieved via a questionnaire.ResultsPatients with florid CS had significantly reduced smell (total smell score 30.3 vs. 34.4,p < 0.0005) and taste scores (9.5 vs. 12.0,p < 0.0005) compared to controls and significantly more frequently hyposmia (55 vs. 2.5%,p < 0.0005), hypogeusia (40 vs. 0%,p < 0.0005), and self-reported chemosensory impairment (60 vs. 0%,p < 0.0005). Three months after successful surgery, CS patients showed significant improvement of odor threshold (8.1 vs. 7.0,p < 0.0005), odor discrimination (12.0 vs. 11.0,p = 0.003), total smell score (33.4 vs. 30.3,p < 0.0005), and taste (11.5 vs. 9.5,p = 0.003).ConclusionsChemosensory dysfunction is a novel and clinically relevant feature of CS.
Adrenal cancer: relevance of different grading systems and subtypes
Authors: Minner S. , Schreiner J. , Saeger W. 
Published: April 2021

AbstractPurposeThe subclassification of adrenal cancers according to the WHO classification in ordinary, myxoid, oncocytic, and sarcomatoid as well as pediatric types is well established, but the criteria for each subtype are not sufficiently determined and the relative frequency of the different types of adrenal cancers has not been studied in large cohorts. Therefore, our large collection of surgically removed adrenal cancers should be reviewed o establish the criteria for the subtypes and to find out the frequency of the various types.MethodsIn our series of 521 adrenal cancers the scoring systems of Weiss et al., Hough et al., van Slooten et al. and the new Helsinki score system were used for the ordinary type of cancer (97% of our series) and the myxoid type (0.8%). For oncocytic carcinomas (2%), the scoring system of Bisceglia et al. was applied.ResultsDiscrepancies between benign and malignant diagnoses from the first thee classical scoring systems are not rare (22% in our series) and could be resolved by the Helsinki score especially by Ki-67 index (more than 8% unequivocally malignant). Since all our cancer cases are positive in the Helsinki score, this system can replace the three elder systems. For identification of sarcomatoid cancer as rarest type in our series (0.2%), the scoring systems are not practical but additional immunostainings used for soft tissue tumors and in special cases molecular pathology are necessary to differentiate these cancers from adrenal sarcomas. According to the relative frequencies of the different subtypes of adrenal cancers the main type is the far most frequent (97%) followed by the oncocytic type (2%), the myxoid type (0.8%) and the very rare sarcomatoid type (0.2%).ConclusionsThe Helsinki score is the best for differentiating adrenal carcinomas of the main, the oncocytic, and the myxoid type in routine work. Additional scoring systems for these carcinomas are generally not any longer necessary. Signs of proliferation (mitoses and Ki-67 index) and necroses are the most important criteria for diagnosis of malignancy.
Beyond Geodiversity Sites: Exploring the Educational Potential of Widespread Geological Features (Rocks, Minerals and Fossils)
Authors: Wolniewicz Paweł 
Published: April 2021

AbstractGeosite and geodiversity site inventories are among the most important means of geological diversity conservation and promotion. However, there are other in situ geological features that have significant educational potential and are not included in many inventories, namely, localities of widespread rock types, common minerals and fossil-bearing strata. In this paper, a broad utilisation of these petrographic, mineralogical and palaeontological geodiversity elements for geoscience communication purposes is postulated, with a case study that focuses on the geological heritage of Poland. A simple quantitative framework for the evaluation of the educational potential of rock types is used for the assessment of preselected geological units on the geological map of Poland. The preferences of potential geotourists are estimated using the interactive web-based map. The promotional materials are written for the most distinctive rock types and geological units that scored the highest in the assessment procedure and/or were most frequently selected by users. This procedure stimulates geodiversity promotion in areas where few geosites and geodiversity sites are documented and no educational activities or interpretative facilities are available, potentially increasing the number of geotourism destinations. The rocks and minerals utilised here are exposed over large areas and can be sampled and studied by untrained collectors without any loss of geodiversity. Shifting the involvement of individuals interested in geosciences from extraordinary to more common rocky outcrops helps to protect the geological heritage and enhances conservation of the most spectacular features for future generations. Field activities such as individual searching and studying outcrops, in turn, play an important role in learning in geosciences, facilitating the acquisition of knowledge and encouraging interdisciplinary thinking. Future improvements could include expanding the applicability of the evaluation method, employment of a location-based learning approach #and more detailed studies of the preferences of potential geotourists.
Heat-Killed Lactic Acid Bacteria Inhibit Nitric Oxide Production via Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cells
Authors: Kang Chang-Ho , Kim Jin-Seong , Kim Hyemin , Park Hye Min , Paek Nam-Soo 
Published: April 2021

AbstractHeat-killed lactic acid bacteria perform immunomodulatory functions and are advantageous as probiotics, considering their long product shelf-life, easy storage, and convenient transportation. In this study, we aimed to develop appropriate heat treatments for industrial preparation of probiotics with antioxidant activity. Among 75 heat-killed strains, Lactococcus lactis MG5125 revealed the highest nitric oxide inhibition (86.2%), followed by Lactobacillus acidophilus MG4559 (86.0%), Lactobacillus plantarum MG5270 (85.7%), Lactobacillus fermentum MG4510 (85.3%), L. plantarum MG5239 (83.9%), L. plantarum MG5289 (83.2%), and L. plantarum MG5203 (81.8%). Moreover, the heat-killed selected strains markedly inhibited lipopolysaccharide-induced nitric oxide synthase and cyclooxygenase-2 expression. The use of heat-killed bacteria with intact bio-functionality can elongate the shelf-life and simplify the food processing steps of probiotic foods, given their high stability. The antioxidant and immune-modulatory activities of the heat-killed strains selected in this study indicate a strong potential for their utilization probiotic products manufacturing.
Numerical analysis of high-lift configurations with oscillating flaps
Authors: Ruhland Johannes , Breitsamter Christian 
Published: April 2021

AbstractThis study presents two-dimensional aerodynamic investigations of various high-lift configuration settings concerning the deflection angles of droop nose, spoiler and flap in the context of enhancing the high-lift performance by dynamic flap movement. The investigations highlight the impact of a periodically oscillating trailing edge flap on lift, drag and flow separation of the high-lift configuration by numerical simulations. The computations are conducted with regard to the variation of the parameters reduced frequency and the position of the rotational axis. The numerical flow simulations are conducted on a block-structured grid using Reynolds Averaged Navier Stokes simulations employing the shear stress transport $$k-\omega $$

k
-
ω

turbulence model. The feature Dynamic Mesh Motion implements the motion of the oscillating flap. Regarding low-speed wind tunnel testing for a Reynolds number of $$0.5 \times 10^{6}$$

0.5
×

10
6

the flap movement around a dropped hinge point, which is located outside the flap, offers benefits with regard to additional lift and delayed flow separation at the flap compared to a flap movement around a hinge point, which is located at 15 % of the flap chord length. Flow separation can be suppressed beyond the maximum static flap deflection angle. By means of an oscillating flap around the dropped hinge point, it is possible to reattach a separated flow at the flap and to keep it attached further on. For a Reynolds number of $$20 \times 10^6$$

20
×

10
6

, reflecting full scale flight conditions, additional lift is generated for both rotational axis positions.
Semantic role labeling for knowledge graph extraction from text
Authors: Alam Mehwish , Gangemi Aldo , Presutti Valentina , Reforgiato Recupero Diego 
Published: April 2021

AbstractThis paper introduces , a new semantic role labeling method that transforms a text into a frame-oriented knowledge graph. It performs dependency parsing, identifies the words that evoke lexical frames, locates the roles and fillers for each frame, runs coercion techniques, and formalizes the results as a knowledge graph. This formal representation complies with the frame semantics used in Framester, a factual-linguistic linked data resource. We tested our method on the WSJ section of the Peen Treebank annotated with VerbNet and PropBank labels and on the Brown corpus. The evaluation has been performed according to the CoNLL Shared Task on Joint Parsing of Syntactic and Semantic Dependencies. The obtained precision, recall, and F1 values indicate that TakeFive is competitive with other existing methods such as SEMAFOR, Pikes, PathLSTM, and FRED. We finally discuss how to combine TakeFive and FRED, obtaining higher values of precision, recall, and F1 measure.
Effect of Process Parameters on Extraction of Pectin from Sweet Lime Peels
Authors: Siddiqui Arshi , Chand Khan , Shahi N. C. 
Published: April 2021

AbstractPectin is a heterogeneous polysaccharide found in the cell wall, middle lamellae of many fruits and vegetables, and is widely used as gelling agent, emulsifier and stabilizer in food industry. In keeping the importance of pectin in the food industry, ultrasound-assisted extraction of pectin from sweet lime peel was investigated. The individual and interactive effect of solid-solvent ratio, ultrasound power intensity and sonication time on the pectin yield, equivalent weight and methoxyl content were studied and analyzed using multiple regression analysis. The maximum pectin yield (36.4 g/100 g), equivalent weight (740.3 mg) and methoxyl content (7.1%) was obtained under optimal condition (solid-solvent ratio of 1:24.3 g/ml, ultrasound power intensity of 80 W/cm2 and sonication time of 18.4 min). Significant (p < 0.05) effect of process parameters was found in all responses of pectin extracted from sweet lime peel. It was also revealed from the present study that ultrasound-assisted extraction of pectin from sweet lime peel is an efficient approach toward waste valorization and extraction of pectin with high yield and good quality attributes.