bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal N-glycosylated CPXM1 promotes gastric cancer metastasis through ITGB2 / FAK pathway
Authors: Du Jun , Zhu Mengxiang , Yan Wenwu , Wang Jin Guo 
Published: June 2021

Abstract
Background: To explore the molecular mechanism of CPXM1 in gastric cancer (GC) metastasis.Methods: Based on the difference in expression of CPXM1 mRNA in GC tissues and normal gastric mucosa tissues in public databases, rt-PCR, WB and IHC staining experiments were performed to verify the expression of CPXM1 in fresh GC tissues. Bioinformatics analysis predicts the possible signal pathways of co-expression genes in CPXM1 overexpression group. The malignant phenotypes of GC cells was verified by in vivo and in vitro experiments. Using tunicamycin to inhibit the N-glycosylation of CPXM1 and constructing mutations. The plasmid further validated the specific N-glycosylation sites that affect the malignant phenotype of gastric cancer cells.Results: CPXM1 mRNA expression was significantly increased in GC tissues. The results of IHC staining and WB indicated that there was no difference in the expression of CPXM1 at the protein level. Knocking down CPXM1 expression in HGC-27 cells can significantly inhibit its migration and invasion ability. Increasing the expression of CPXM1 in MGC-803 and BGC-823 cell lines can significantly promote its migration and invasion capabilities. CPXM1 can also promote the adhesion of GC cells and change the cytoskeleton structure. The results of in-vivo experiments show that CPXM1 can promote the metastasis of GC cells in the abdominal cavity of nude mice. Bioinformatics analysis showed that the co-expressed genes of the CPXM1 high expression group were mainly enriched in cell adhesion function, and the experimental results verified that CPXM1 may promote the adhesion and metastasis of gastric cancer cells through the ITGB2/FAK signaling pathway. Through N-glycosylation site prediction and WB results, it was found that CPXM1 had abnormal N-glycosylation in GC tissues. Tunicamycin can reverse the ability of CPXM1 that promote the invasion of GC cells. After respectively mutating the N-glycosylation sites on CPXM1, it was found that N210 site is the key site for CPXM1 to promote the adhesion and invasion of GC cells.Conclusion: CPXM1 is upgraded in GC. CPXM1 can promote the adhesion, migration and invasion of GC cells and change cytoskeleton structure. The N210 site on CPXM1 is a key site affecting the malignant phenotype of GC.
Association between blood glucose levels in insulin therapy and Glasgow Outcome Score in patients with traumatic brain injury: secondary analysis of a randomized trial
Authors: Yuan Tao , He Hongyu , Liu Yuepeng , Wang Jianwei , Kang Xin , Fu Guanghui , Xie Fangfang , Li Aimin , Chen Jun , Wang Wen-xue 
Published: June 2021

Abstract
Background: Too high or low blood glucose levels after traumatic brain injury (TBI) negatively affect the prognosis of patients with TBI. This study aimed to examine the relationship between different levels of blood glucose in insulin therapy and Glasgow Outcome Score (GOS) in patients with TBI.Methods: This study was based on a randomized, dual-center, open-label clinical trial. A total of 208 patients who participated in the randomized controlled trial were followed up for 5 years. The information on disease, lab examination, insulin therapy, and operation of patients with TBI was collected. Also, the data on 5-year and 6-month GOS were collected as primary and secondary outcomes. The univariate analysis was used to detect variables that might contribute to outcomes, while the multivariate regression analysis was used to reveal the independent relationship between insulin therapy and outcomes. A generalized additive model (GAM) was used to investigate dose–response relationships between blood glucose levels and GOS. The results were presented as odds ratios (ORs) with their 95% confidence intervals (95% CIs). We further applied a two-piecewise linear regression model to examine the threshold effect of blood glucose level and GOS.Results: A total of 182 patients were involved in the final analysis. Compared with the non-intensive insulin therapy group, the 5-year GOS in the moderate-control intensive insulin therapy (IIT) group and the slight-control IIT group was 1.45 and 1.39 higher, respectively (both P < 0.05); the 5-year GOS in the strict-control IIT group was 0.23 higher (P > 0.05). GAM revealed that a bell-shaped relationship existed between blood glucose levels in insulin therapy and 5-year or 6-month GOS. The inflection points of the mean blood glucose level were 6.73 and 8.97 mmol/L considering 5-year GOS as the outcome and were 6.95 and 8.88 mmol/L considering 6-month GOS as the outcome. The multivariate analysis showed that the 5-year GOS in the medium-level group (≥6.73 and <8.97 mmol/L) increased by 0.83 [95% confidence interval (CI): 0.22–1.47] compared with that in the low-level group (<6.73 mmol/L). Also, the 5-year GOS in the medium-level group increased by 0.57 (95% CI: 0.02–1.08) compared with that in the high-level group (≥8.97 mmol/L).Conclusion: A proper blood glucose range in insulin therapy could improve the outcomes of patients with TBI; the range was 6.73–8.97 mmol/L according to our limited analysis.Clinical Trial Registration: ClinicalTrials.gov, NCT02161055. Registered 11 Jun 2014.
Asymmetric Effects of Cereals Crops on Agricultural Economic Growth: A Case Study of India
Authors: Bansal Surbhi , Kumar Pushp , Mohammad Shan , Ali Nazim , Ansari Mohd Arshad 
Published: June 2021

Abstract
The study explores the asymmetric effects of cereals crops, namely wheat, rice, and maize production on agricultural economic growth in India during 1960–2019. The asymmetric ARDL method is used in this study to analyze the asymmetric relationship between the independent and dependent variables. The findings reveal a link between cereal crop growth and agricultural economic growth. The NARDL findings indicate that positive maize and rice shocks have a considerable short- and long-term influence on agricultural economic growth. In contrast, the positive shock of wheat production is not significant. While the negative shocks of maize, rice and wheat production significantly impact agricultural economic growth. In a nutshell, the study reveals that agriculture growth has an asymmetric relationship with maize, wheat and rice production. The study's findings imply that policymakers should develop long- and short-term plans to boost agricultural growth and productivity in order to help farmers.
Elevator (Lift) Car Ventilation Design to Mitigate the Risk of Spread of COVID-19
Authors: Tipnis Pravin M. , Vaidya Vinay G. 
Published: June 2021

Abstract
Recent studies, on the airborne transmission of the SARS-CoV-2 and the new CDC guidelines confirming the aerosol transmission of the virus, make immediate attention to the airflow in elevators imperative. While several versions have been thought of for contactless rides, there is little that has been considered for lessening the anticipated viral load in the elevator car.In this paper, a two-step approach is used. The first is the risk assessment, and the second is the risk mitigation through an improved lift car ventilation design. The risk is assessed by computing the probable viral load during the journey in a lift car. It is seen that the ventilation typically provided as per the minimum permissible requirements by codes is inadequate to handle the current situation.The computations show that one-minute exposure, to a coughing high emitter in the lift car, creates a viral load to dangerous levels well above the possible acceptable level of 10,000 virus copies/m3. To come up with the risk mitigation strategies, the required ventilation in the car was computed. The main recommendation suggests the use of forced ventilation using pressure fans and 3 to 6 air changes per minute depending on the operating environment. Such a design will help us achieve our objective of bringing the density of virus copies in the lift car to an acceptable level. A properly designed ventilation system for the elevator car will benefit in both pandemic situations as well as non-pandemic situations.
Assessing the Reliability and Validity of an Instrument for Measuring Patient Use of Telemedicine in Light of the Technology Acceptance Model
Authors: Althumairi Arwa 
Published: June 2021

Abstract
Background:Telemedicine has been used as a supportive service to assure that quality healthcare services are provided in a timely manner. The growing use of telemedicine, especially for e-consultations, is increasing patient access to care, especially during pandemics or emergencies. However, a valid and reliable tool is needed to measure its use. This study aimed to evaluate the reliability and validity of patient acceptance of telemedicine, utilizing the dimensions of Technology Acceptance Model (TAM).Methods:The researcher designed an instrument based on TAM dimensions/principles. The instrument was distributed among 244 participants via an online link found in social media, such as Twitter and WhatsApp, from August 2020 to December 2020. Content validity and exploratory and confirmatory factor analysis were used to examine the construct validity, and Cronbach’s alpha test was employed to examine the reliability of the instrument.Results:A total of 244 participants in the study and 22 items or questions had a loading factor greater than 60. The assessment suggested 6 factors to explain the variation in the participant responses, with 75% of the variation being explained by these factors. The confirmatory analysis agreed with the explanatory factors, suggesting that the number of items and latent factors had a CMIN/DF equal to 3.021 and an RMSEA of 0.095, with a 90% CI (0.105 -0.086).Conclusion:The results of the study suggest that the measure shows acceptable validity and reliability.
Gasless laparoscopy versus conventional laparoscopy and laparotomy. A systematic review on the safety and efficiency
Authors: Shoman Haitham , Sandler Simone , Peters Alexander , Farooq Ameer , Gruendl Magdalen , Trinh Shauna , Little James , Woods Alex , Bolton William , Abioye Abubakar , Ljungman David 
Published: June 2021

Abstract
Background Gasless laparoscopy emerged to overcome the clinical and financial challenges of pneumoperitoneum and is often seen as a viable option for use in resource-limited settings as a means of saving costs and resources. This study aims to systematically review the evidence available on the safety and efficiency of gasless laparoscopy compared to conventional laparoscopy and laparotomy.Methods Following PRISMA guidelines, Medline, Embase, Web of Science and Cochrane were searched. Variables of interest were determined a priori and Covidence software was used to screen studies for inclusion without demographic preference. The quality of studies was assessed using the Cochrane Risk Assessment tool.Results Of 1080 screened studies a total of 43 studies were included. Laparoscopic cholecystectomy was by far the most studied intervention in randomized studies. In these, the mean set-up time for gasless and conventional laparoscopy was 13.14 (95% CI: -0.16–26.44) and 12.8 (95% CI: -10.86–36.47) minutes respectively; The mean duration of surgery for gasless and conventional laparoscopy was 89.39 (95% CI: 77.44-101.34) and 72.59 (95% CI: 63.44–81.74) minutes respectively and the mean length of stay was 4.25 (95% CI: 2.02–6.48) and 4.04 (95% CI: 1.72–6.36) days respectively. Most reported complications were hemorrhage and infection with no assessable statistical difference.Conclusions Although gasless laparoscopy seems to be a feasible approach for many general surgery interventions, the observed outcomes based on safety and efficiency don’t suffice to recommend gasless laparoscopy as an alternative to conventional laparoscopy or laparotomy. Larger randomized trials with a low risk of bias are warranted.
Latrine characteristics and maintenance practices associated with pit latrine lifetime in an informal settlement in Kampala, Uganda
Authors: Mubatsi Johnson B. , Wafula Solomon Tsebeni , Etajak Samuel , Ssekamatte Tonny , Isunju John Bosco , Kimbugwe Ceaser , Olweny Moses , Kayiwa Denis , Mselle Jane Sembuche , Halage Abdullah Ali , Ssempebwa John C. , Mugambe Richard K. 
Published: June 2021

Abstract
This study assessed latrine characteristics and maintenance practices associated with an extended pit latrine lifetime in an informal settlement in Kampala, Uganda. Data were obtained from 306 respondents on sociodemographic characteristics, their private pit latrine characteristics and latrine lifetime. A modified Poisson regression was used to model the latrine characteristics and maintenance practices associated with the pit latrine lifetime. All analyses were performed using Stata 14 software. Approximately 23.5% of the pit latrines had a lifetime of less than 2 years, and most latrines were reportedly desludged (64.7%) or regularly cleaned for maintenance (27.1%) as a way of extending lifetime. Pit latrine lifetime extension was higher in male-headed households (prevalence ratio (PR) 1.12, 95% confidence interval (CI) 1.00–1.25), households with a smaller number of users (unshared vs shared latrines) (PR 1.15, 95% CI 1.02–1.29) and where desludging of pits was regularly done (PR 1.53, 95% CI 1.17–1.99), while post-primary education level was negatively associated with extended latrine lifetime (PR 0.88, 95% CI 0.77–0.99). Deliberate efforts, including sensitizing communities on desludging and provision of non-shared household sanitary facilities, are needed to improve latrine maintenance and consequently extend latrine lifetime.
Scenario-based life-cycle cost assessment to support sustainable investment in rural communal sanitation facilities: application to a school-based sanitation facility
Authors: Koseoglu N. M. , Ellis R. , Biswas D. 
Published: June 2021

Abstract
Despite decades of investment in widening access to improved sanitation, much of the world still lacks access to functional sanitation facilities. Through much of the Global South, toilets are inoperable and often abandoned. Failure to understand and account for the whole-life cost of sanitation infrastructure, as well as the interplay between context-specific socio-economic determinants, is one explanation for this reduction in the service life of shared sanitation infrastructure. This issue is especially salient in school-based and communal facilities in middle- to low-income countries. Drawing on a case study of a sanitation facility in a government school in rural south India, we explore the relationship between user value, community-based capacity, and external support in determining the costs of operating and maintaining sanitation facilities over their lifetime. We develop a scenario-based life-cycle cost assessment methodology to examine the relational impact of these determinants on the ‘real’ cost of shared sanitation infrastructure. The analysis concludes that investment and interventions that stimulate demand and enhance the capacity of a community are the most cost-effective options for ensuring the sustainability of sanitation facilities in our case study site. We then reflect on the applicability and limitations of these findings for a wider range of communal sanitation facilities.
Flood season segmentation and scheme optimization in the Yellow River
Authors: Zhou Ke 
Published: June 2021

Abstract
River flood season segmentation is a significant measure for flood prevention. This study aims to carry out theoretical analysis on flood season segmentation methods and put forward a framework for proper flood season segmentation through comparison between different segmentation methods. The studied framework consists of a Fisher optimal partition method for determining the optimum numbers of the sub-seasons, an ensemble approach for segmenting a defined flood season, and a nonparametric bootstrap combined with a fuzzy optimum selection method (NPB-FOS) for testing the rationality of the flood season staging schemes. The present research findings show that different methods could result in different staging schemes. It is proved through rational analysis that the staging scheme obtained by probability change point (PCP) is superior to others. The flood season of the downstream reach of the Yellow River can be segmented into three sub-seasons, i.e. early flood season (01 June–20 July), main flood season (21 July–28 September), and late flood season (29 September–08 November). The segmentation results of the flood season should play an active role in flood prevention.
Analisis Potensi Likuefaksi dan Perbaikan Tanah dengan Stone Column: Studi Kasus pada Coal Shelter PLTU Lontar, Banten
Authors: Fajarwati Yuli , Kusuma Rama Indera 
Published: June 2021

ABSTRACT The high demand for electricity needs requires the availability of new generation sites. The new plant developed is a PLTU in Lontar. The condition of coal shelter as a research site dominated by silty clay and silty sand and located in the earthquake zoning is high that the planning of the coal shelter area must accordance with the feasibility of building establishment, it is necessary to analyze the potential liquefaction and improvement methods. Soil improvement efforts to reduce the potential for liquefaction include soil improvement with stone columns. The method of liquefaction analysis in this study uses the method developed by Idriss and Boulanger. The results of the analysis of the potential for liquefaction at BH-1 occurred at a depth of 3-16 m and at BH-3 the potential for liquefaction occurred at a depth of 4-24 m. Potential of the thickest layer for liquefaction is at BH-3 with a depth of 24 m. Improvement with a stone column can reduce the potential for liquefaction and can increase the value of the safety factor against the potential for liquefaction at the coal shelter location. Improvement analysis with a stone column using Plaxis software, the value of the safe factor after installing the stone column at BH-1 FS 2.89, at BH-3 FS became 2.65. ABSTRAKBanyaknya permintaan kebutuhan listrik yang tinggi diperlukan ketersediaan lokasi pembangkit baru. Pembangkit baru yang dikembangkan yaitu PLTU Batubara di Lontar. Kondisi coal shelter sebagai lokasi penelitian yang didominasi oleh lapisan tanah lempung kelanauan dan pasir kelanauan serta berada pada zonasi gempa cukup tinggi sehingga perencanaan area coal shelter harus memenuhi syarat kelayakan pendirian bangunan, maka perlu dilakukan analisis potensi likuefaksi serta metode perbaikannya. Upaya perbaikan tanah untuk mengurangi potensi likuefaksi yaitu perbaikan tanah dengan stone column (kolom batu). Metode analisis likuefaksi pada penelitian ini menggunakan metode yang dikembangkan oleh Idriss dan Boulanger. Hasil analisis potensi likuefaksi pada titik BH-1 terjadi di kedalaman 3-16 m dan pada titik BH-3 potensi likuefaksi terjadi pada kedalaman 4-24 m. Lapisan yang paling tebal mengalami potensi likuefaksi ada pada titik BH-3 dengan kedalaman 24 m. Perbaikan dengan stone column dapat mengurangi potensi likuefaksi dan mampu meningkatkan nilai faktor keamanan terhadap potensi likuefaksi di lokasi coal shelter. Analisis perbaikian dengan stone column menggunakan software Plaxis, nilai faktor aman setelah dipasang stone column pada titik BH-1 FS 2,89, pada titik BH-3 FS menjadi 2,65.
Professional Development for Vocational High School Teachers through Incremental Teacher Competence Standards
Authors: Waluyanti Sri , Santoso Djoko , Munir Muh , Wulandari Bekti , Raduan Atiqah 
Published: June 2021

This study presents the competence of vocational high school teachers through the implementation of five work dimensions of teachers’ incremental standard competence. It was conducted in the framework of the sustainable professional development for vocational teachers using participatory action research at two Vocational High Schools by involving 50 teachers. The study included socialization of incremental competency standards and reflection, mentoring and consultation sessions, and teaching reports and reflection. Data collection used observation, assessment, and teaching reports. To optimize the results, it was ended with a focus group discussion involving the Institute for Education Quality Assurance. The results showed that the teachers’ competence in the aspects of the learning plan, learning resource utilization, media utilization, facilitating students’ learning needs, ability to create learning evaluation instrument, and following up the learning outcomes were all categorized as good while the aspect of developing students’ potentials both curriculum implementation and reflection ability was categorized as very good. It indicates the mentoring and the consultation worked effectively. Meanwhile, the teachers' ability that should be improved is the creation of assessment instruments.
Profiles of Teachers’ Expertise and Professional Development in Technology and Engineering Expertise Program of Vocational Secondary Schools
Authors: Djatmiko Istanto Wahyu , Waluyanti Sri , Sukardi Thomas , Yuswono Lilik Chaerul 
Published: June 2021

This study was aimed to identify the profiles of teachers' expertise and professional development in Technology and Engineering Expertise Program (TEEP) of Vocational Secondary Schools (VSSs). This study was conducted with a survey approach. The population consisted of 1025 teachers of TEEP at VSSs in Yogyakarta, Indonesia. The samples were 280 teachers selected with proportional random sampling. Data were collected by an open-ended questionnaire and analyzed with a descriptive method. Results of the study identified the profiles of expertise that included the majority of teacher’s employment status is government employees and scanty non-government employees. The range of working time is mostly from 7 and 18 years. Teachers mostly participate in the teachers’ certification through the professional teachers training program. A few number of the teachers have the additional expertise certificates, and none of the teachers has the dual expertise certificates. Furthermore, types of the teachers’ professional development activities that followed by most of the teachers are seminars, journals, learning media, and participating as members of teachers association.