bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure to arsenic at different life-stages and DNA methylation meta-analysis in buccal cells and leukocytes
Authors: Bozack Anne K. , Boileau Philippe , Wei Linqing , Hubbard Alan E. , Sillé Fenna C. M. , Ferreccio Catterina , Acevedo Johanna , Hou Lifang , Ilievski Vesna , Steinmaus Craig M. , Smith Martyn T. , Navas-Acien Ana , Gamble Mary V. , Cardenas Andres 
Published: July 2021

AbstractBackgroundArsenic (As) exposure through drinking water is a global public health concern. Epigenetic dysregulation including changes in DNA methylation (DNAm), may be involved in arsenic toxicity. Epigenome-wide association studies (EWAS) of arsenic exposure have been restricted to single populations and comparison across EWAS has been limited by methodological differences. Leveraging data from epidemiological studies conducted in Chile and Bangladesh, we use a harmonized data processing and analysis pipeline and meta-analysis to combine results from four EWAS.MethodsDNAm was measured among adults in Chile with and without prenatal and early-life As exposure in PBMCs and buccal cells (N = 40, 850K array) and among men in Bangladesh with high and low As exposure in PBMCs (N = 32, 850K array;N = 48, 450K array). Linear models were used to identify differentially methylated positions (DMPs) and differentially variable positions (DVPs) adjusting for age, smoking, cell type, and sex in the Chile cohort. Probes common across EWAS were meta-analyzed using METAL, and differentially methylated and variable regions (DMRs and DVRs, respectively) were identified using comb-p. KEGG pathway analysis was used to understand biological functions of DMPs and DVPs.ResultsIn a meta-analysis restricted to PBMCs, we identified one DMP and 23 DVPs associated with arsenic exposure; including buccal cells, we identified 3 DMPs and 19 DVPs (FDR < 0.05). Using meta-analyzed results, we identified 11 DMRs and 11 DVRs in PBMC samples, and 16 DMRs and 19 DVRs in PBMC and buccal cell samples. One region annotated toLRRC27was identified as a DMR and DVR. Arsenic-associated KEGG pathways included lysosome, autophagy, and mTOR signaling, AMPK signaling, and one carbon pool by folate.ConclusionsUsing a two-step process of (1) harmonized data processing and analysis and (2) meta-analysis, we leverage four DNAm datasets from two continents of individuals exposed to high levels of As prenatally and during adulthood to identify DMPs and DVPs associated with arsenic exposure. Our approach suggests that standardizing analytical pipelines can aid in identifying biological meaningful signals.
The mechanisms of action of Plasmodium infection against cancer
Authors: Chen Xiaoping , Qin Li , Hu Wen , Adah Dickson 
Published: July 2021

AbstractOur murine cancer model studies have demonstrated that Plasmodium infection activates the immune system that has been inhibited by cancer cells, counteracts tumor immunosuppressive microenvironment, inhibits tumor angiogenesis, inhibits tumor growth and metastasis, and prolongs the survival time of tumor-bearing mice. Based on these studies, three clinical trials of Plasmodium immunotherapy for advanced cancers have been approved and are ongoing in China. After comparing the mechanisms of action of Plasmodium immunotherapy with those of immune checkpoint blockade therapy, we propose the notion that cancer is an ecological disease and that Plasmodium immunotherapy is a systemic ecological counterattack therapy for this ecological disease, with limited side effects and without danger to public health based on the use of artesunate and other measures. Recent reports of tolerance to treatment and limitations in majority of patients associated with the use of checkpoint blockers further support this notion. We advocate further studies on the mechanisms of action of Plasmodium infection against cancer and investigations on Plasmodium-based combination therapy in the coming future.
Sleep duration, physical activity, and caloric intake are related to weight status in Mexican American children: a longitudinal analysis
Authors: Martinez S. M. , Blanco E. , Tschann J. M. , Butte N. F. , Grandner M. A. , Pasch L. A. 
Published: July 2021

Abstract
Background
Obesity is a serious issue, spanning all ages, and, in the U.S., disproportionately affects Latinos and African Americans. Understanding sleep, physical activity and dietary behaviors that may predict childhood obesity can help identify behavioral intervention targets.

Methods
Data were drawn from a U.S. cohort study of 323 Mexican American 8–10-year-old children and their mothers, who participated in a longitudinal study over a 2-year period. Measures were collected at baseline (BL; child mean age = 8.87, SD = 0.83), year 1 (FU1) and year 2 (FU2). Mothers reported on household income and acculturation at BL. Child height and weight were collected and BMI z-scores (BMIz) were calculated for weight status at BL, FU1, and FU2. Accelerometer-estimated sleep duration (hours) and moderate-to-vigorous physical activity (MVPA; minutes) were collected across 3 days at BL, FU1, and FU2. Two 24-h dietary recalls were performed at each time point; from these, average energy intake (EI, kcals/day) was estimated. Cross-lagged panel analysis was used to examine behavioral predictors on BMIz at each time point and across time.

Results
At BL and FU1, longer sleep duration (β = − 0.22, p < 0.001; β = − 0.17, p < 0.05, respectively) and greater MVPA (β = − 0.13, p < 0.05; β = − 0.20, p < 0.01, respectively) were concurrently related to lower BMIz. At FU2, longer sleep duration (β = − 0.18, p < 0.01) was concurrently related to lower BMIz, whereas greater EI (β = 0.16, p < 0.01) was related to higher BMIz. Longer sleep duration at BL predicted lower BMIz at FU1 (β = − 0.05, p < 0.01).

Conclusions
Longer sleep duration was concurrently related to lower weight status at each time point from ages 8–10 to 10–12. Higher MVPA was concurrently related to lower weight status in earlier childhood (ages 8–10 and 9–11) and higher EI was concurrently related to higher weight status toward the end of childhood (ages 10–12 years). Furthermore, longer sleep in earlier childhood was protective of children’s lower weight status 1 year later. These findings suggest that sleep duration plays a consistent and protective role against childhood obesity; in addition, MVPA and healthy EI remain important independent factors for obtaining a healthy weight.
From Ebola to COVID-19: emergency preparedness and response plans and actions in Lagos, Nigeria
Authors: Abayomi Akin , Balogun Mobolanle R. , Bankole Munir , Banke-Thomas Aduragbemi , Mutiu Bamidele , Olawepo John , Senjobi Morakinyo , Odukoya Oluwakemi , Aladetuyi Lanre , Ejekam Chioma , Folarin Akinsanya , Emmanuel Madonna , Amodu Funke , Ologun Adesoji , Olusanya Abosede , Bakare Moses , Alabi Abiodun , Abdus-Salam Ismail , Erinosho Eniola , Bowale Abimbola , Omilabu Sunday , Saka Babatunde , Osibogun Akin , Wright Ololade , Idris Jide , Ogunsola Folasade 
Published: July 2021

Abstract
Background
Lagos state is the industrial nerve centre of Nigeria and was the epicentre of the 2014 Ebola outbreak in Nigeria as it is now for the current Coronavirus Disease (COVID-19) outbreak. This paper describes how the lessons learned from the Ebola outbreak in 2014 informed the emergency preparedness of the State ahead of the COVID-19 outbreak and guided response.

Discussion
Following the Ebola outbreak in 2014, the Lagos State government provided governance by developing a policy on emergency preparedness and biosecurity and provided oversight and coordination of emergency preparedness strategies. Capacities for emergency response were strengthened by training key staff, developing a robust surveillance system, and setting up a Biosafety Level 3 laboratory and biobank. Resource provision, in terms of finances and trained personnel for emergencies was prioritized by the government. With the onset of COVID-19, Lagos state was able to respond promptly to the outbreak using the centralized Incident Command Structure and the key activities of the Emergency Operations Centre. Contributory to effective response were partnerships with the private sectors, community engagement and political commitment.

Conclusion
Using the lessons learned from the 2014 Ebola outbreak, Lagos State had gradually prepared its healthcare system for a pandemic such as COVID-19. The State needs to continue to expand its preparedness to be more resilient and future proof to respond to disease outbreaks. Looking beyond intra-state gains, lessons and identified best practices from the past and present should be shared with other states and countries.
A pragmatic window of opportunity to minimise the risk of MRONJ development in individuals with osteoporosis on Denosumab therapy: a hypothesis
Authors: Campisi Giuseppina , Mauceri Rodolfo , Bertoldo Francesco , Fusco Vittorio , Bedogni Alberto 
Published: July 2021

Abstract
Denosumab is associated with the development of medication-related osteonecrosis of the jaw (MRONJ), an uncommon but severe oral side effect with a higher prevalence in metastatic cancer patients than in patients with metabolic bone fragility. Although several oral triggers can initiate MRONJ, invasive oral treatments and tooth extraction still remain the most common precipitating event. In general, tooth extraction and oral surgery should be avoided in patients at increased risk of MRONJ, while extraction of non-restorable teeth should be performed based on specific risk reduction protocols to eliminate dental/periodontal infections, still protecting from MRONJ onset.
Based on the different pharmacological activity of denosumab and nitrogen-containing bisphosphonates, it is likely that the MRONJ risk profile of patients with osteoporosis could somewhat vary.
We hypothesize the chance to maximize the pharmacokinetic of denosumab 60 mg (Prolia®) and identify a time interval in which invasive oral treatments can ideally take place without restrictions in patients with metabolic bone fragility,
We propose that dental surgery (e.g. tooth extraction) may be safely performed without additional intra or peri-operative procedures in osteoporosis patients using denosumab provided that careful case selection, adequate communication among specialists, planning of a delayed dosing window (1-month deferral) and rigorous postoperative follow-up are granted.

Graphical abstract
The relationship between thoracic kyphosis and age, and normative values across age groups: a systematic review of healthy adults
Authors: Zappalá Mattia , Lightbourne Stephen , Heneghan Nicola R. 
Published: July 2021

Abstract
Background
Thoracic kyphosis is reported to increase with ageing. However, this relationship has not been systematically investigated. Peoples’ kyphosis often exceeds 40°, but 40° is the widely accepted cut-off and threshold for normality. Consequently, patients may be misclassified. Accurate restoration of kyphosis is important to avoid complications following spinal surgery. Therefore, specific reference values are needed. The objective of the review is to explore the relationship between thoracic kyphosis and age, provide normative values of kyphosis for different age groups and investigate the influence of gender and ethnicity.

Methods
Two reviewers independently conducted a literature search, including seven databases and the Spine Journal, from inception to April 2020. Quantitative observational studies on healthy adults (18 years of age or older) with no known pathologies, and measuring kyphosis with Cobb’s method, a flexicurve, or a kyphometer, were included. Study selection, data extraction, and study quality assessment (AQUA tool) were performed independently by two reviewers. The authors were contacted if clarifications were necessary. Correlation analysis and inferential statistics were performed (Microsoft Excel). The results are presented narratively. A modified GRADE was used for evidence quality assessment.

Results
Thirty-four studies (24 moderate-quality, 10 high-quality) were included (n = 7633). A positive moderate correlation between kyphosis and age was found (Spearman 0.52, p < 0.05, T5-T12). Peoples’ kyphosis resulted greater than 40° in 65% of the cases, and it was significantly smaller in individuals younger than 40 years old (x < 40) than in those older than 60 years old (x > 60) 75% of the time (p < 0.05). No differences between genders were found, although a greater kyphosis angle was observed in North Americans and Europeans.

Conclusion
Kyphosis increases with ageing, varying significantly between x < 40 and x > 60. Furthermore, kyphosis appears to be influenced by ethnicity, but not gender. Peoples’ thoracic sagittal curvature frequently exceeds 40°.

Trial registration
The review protocol was devised following the PRISMA-P Guidelines, and it was registered on PROSPERO (CRD42020175058) before study commencement.
Principles for the production and dissemination of recruitment material for three clinical trials of an online intervention
Authors: Rennick-Egglestone Stefan 
Published: July 2021

AbstractSome health research studies recruit participants through electronic mechanisms such as the placement of messages on social media platforms. This raises questions for ethics committee oversight, since effective social media campaigns might involve the production and dissemination of hundreds of contemporaneous messages. For the Narrative Experiences Online (NEON) study, we have developed nine principles to control the production and dissemination of promotional material. These have been approved by an ethics committee and enable the audit of our recruitment work. We propose that the drafting for approval of recruitment principles by health research studies may, in many cases, strike an appropriate balance between enabling ethical oversight of online recruitment work and the potential burden of message review.
Effect of temperature and dissolved oxygen on gravity sedimentation of the unicellular alga Dunaliella salina
Authors: Watsuji Tomo-o , Naka Angelica , Morita Yasuhiko , Kurahashi Midori 
Published: July 2021

Abstract
Purpose
The microalgae Dunaliella salina is mainly cultivated as a source of natural β-carotene, but it can also be an important source of other nutrients such as protein, carbohydrate, and lipids. Harvesting and dewatering are considered the most expensive processes in the biomass production, so we proposed gravity sedimentation as a cost-effective method. The effect of temperature and dissolved oxygen concentration on the gravity sedimentation of normal cells and carotenoid-accumulating cells of Dunaliella salina was investigated in this study.

Methods
Dunaliella salina was cultivated in an f/2 culture medium at two different salinities, 12.5% (2.2 M NaCl) and 17.5% (3.3 M NaCl). Carotenoid-accumulating cells were cultivated in a medium without a nitrogen source by removing NaNO3 from the f/2 medium and at two different salinities, 12.5% and 17.5%. For gravity sedimentation tests, 10 mL of the suspended culture media were transferred to conical tubes and wrapped with aluminum foil for shading. The tubes were incubated at 4 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, 35 °C, 40 °C, 45 °C, and 50 °C for 4 h and 18 h. For the gravity sedimentation under aerobic and anaerobic conditions, 10 mL of the suspended culture media were transferred to conical tubes and then purged with 20% oxygen and 80% nitrogen or 100% nitrogen for 10 min and wrapped with aluminum foil. The tubes were incubated at 25 °C for 0.5 h, 1 h, 2 h, and 3 h.

Result
Recovery rates differed with temperature, salinity, and time. The recovery rate of normal cells and carotenoid-accumulating cells reached 79 to 96% at 20 °C, 25 °C, and 30 °C at 12.5% and 17.5% of salinity and after 4 h. The recovery rate of both normal and carotenoid-accumulating cells in gravity sedimentation experiments was not significantly affected by changing initial dissolved oxygen in the cell-cultured media.

Conclusion
Considering that gravity sedimentation at the optimum growth temperature for D. salina presented high recovery rates, gravity sedimentation is expected to be a cost-effective method to harvest D. salina from open pond cultivation systems.
Culture, innovation, and economic development
Authors: Kostis Pantelis C. 
Published: July 2021

AbstractDuring recent decades, culture is gaining more and more attention as a factor that determines economic outcomes. Trying to investigate its role on innovation and economic development, this paper uses a dataset that offers the potential for a cross-sectional and time series analysis. Thus, in this paper, the effects of culture on innovation (as measured by patent applications, spending on R&D, number or researchers per 1000 individuals and number of government researchers) and economic development are investigated. Cultural background is captured through the Schwartz’s cultural values, as reported through the European Social Survey (ESS) waves during the period 2002–2018. The dataset is comprised by 18 Eurozone countries. Using principal component analyses to capture the Schwartz’s cultural values, as well as two ways fixed-effects analysis (FE), time dummies for each ESS wave included in the analysis and cluster—robust estimates of the standard errors, in order to examine the above relationships, the main conclusions derived from the analysis are that (a) there is significant effect of culture on innovation and economic development, and (b) the main cultural dimensions that hinder innovation and economic development are the prevalence of hierarchy, affective autonomy, and mastery. These results hold for all different dependent variables used in the analysis. Thus, when hierarchy, affective autonomy, and mastery are present innovation and economic development are hindered, leading to obstacles regarding the sustainability of economic outcomes. The opposite holds in societies where embeddedness, egalitarianism, and harmony prevail.
Intraosseous schwannoma of the proximal humerus with pathologic fracture
Authors: Huajun Jiang , Wei Qu , Yuxuan Wu , Jingjing Yang 
Published: July 2021

Abstract
Background
Intraosseous schwannomas are extremely rare in the humerus, and less than five cases have been reported previously in the literature. This is the first report of its origin in the proximal humerus with pathologic fracture. We herein present this case to discuss the reason for its rarity and share our experience of management.

Case presentation
A 55-year-old female patient presented with pain in the right shoulder, which was caused by tripping and falling over a board. Radiographs, computed tomography (CT) and magnetic resonance imaging (MRI) showed considerable tumor in proximal humerus, which connected with a fracture. For this suspected tumor, we performed two operations. Pathological examination demonstrated typical picture of a schwannoma, showing whorls and interlacing fascicles of schwannoma spindle cells. Immunohistochemistry, the tumor cells were diffusely positive for S-100 protein, SOX-10 and CD68, while they were completely negative for desmin, DOG-1, AE1/AE3 and P63. The Ki-67 index was about 10%. No mitoses or features of malignancy were identified. The final diagnosis of intraosseous schwannoma was made. The treatment for intraosseous schwannoma with pathologic fracture includes excisional biopsy, curettage, bone allograft, and fracture fixation. The patient recovered well. After the surgery, the patient gradually regained mobility and the pain subsided. There was no recurrence after 6 months of follow-up by X-ray.

Conclusions
Although very rare, intraosseous schwannoma should be taken under consideration in the differential diagnosis of benign-appearing osseous tumor in the proximal humerus with pathologic fracture.
From solidarity to resistance: host communities’ evolving response to the Rohingya refugees in Bangladesh
Authors: Ansar Anas , Md. Khaled Abu Faisal 
Published: July 2021

AbstractBangladesh sets an admirable example of solidarity with the Rohingya refugees from Myanmar by hosting more than a million Rohingyas despite its resource constraints. However, there is a perceptible shift from this rather unconditional solidarity to an anti-refugee sentiment. In evaluating the factors that contributed to these changing dynamics, we analysed how the host communities’ solidarity with the plight of Rohingya refugees evolved. Broadly, it identifies three key factors that influence the approaches of the locals towards the refugees: economic instability as a result of the wage fall and price hike, unequal access to humanitarian aid and uneven distribution of resource opportunities created through substantial humanitarian operations and finally, political uncertainty about the future of the Rohingya crisis. By bringing the experiences of host communities and the manifold implications of existing humanitarian interventions into the centre of the analysis, the paper underlines the need for a more conciliatory approach involving different actors engaged in this crisis. In so doing, we argue that addressing the adverse impacts of a refugee crisis on the poorer hosts, particularly within the context of a protracted refugee situation, needs a more systematic evaluation, and it cannot be dealt with isolation from the broader socio-economic context of the refugee-hosting regions.
Virulence of Beauveria bassiana and Metarhizium anisopliae on different stages of the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)
Authors: Omar Gamal , Ibrahim Ahmed , Hamadah Khalid 
Published: July 2021

Abstract
Background
The pink bollworm, Pectinophora gossypiella, is the most destructive pests of the cotton plant in Egypt. Due to the several problems of insecticides, the present study was conducted to evaluate the toxicity effect of the entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae, against the different stages of the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

Results
The fungal isolates exhibited a toxic effect against the treated stages, egg, larva, and pupa. According to the obtained data of LC50, B. bassiana was more potent in inducing toxicity than M. anisopliae. However, eggs of P. gossypiella were less susceptible to the EPF than the other stages. Based on total mortality, LC50 was 4.97×1011, 6.03×1012 spores/ml for egg; 8.25×108, 6.03×109 spores/ml for neonate; 2.52×108, 1.29×1010 spores/ml for early 4th instar larvae; and 6.79×108, 8.36×109 spores/ml for pupae after treatment with B. bassiana and M. anisopliae, respectively.

Conclusions
Entomopathogenic fungi exhibited an activity in inducing mortality against different stages of P. gossypiella.