bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОРГАНІЗАЦІЙНО-ПЕДАГОГІЧНІ УМОВИ ЗДОБУТТЯ ПРОФЕСІЙНИХ КОМПЕТЕНЦІЙ МАЙБУТНІМИ СУДНОВИМИ МЕХАНІКАМИ
Authors: Герганов Леонід , Липенков Ігор 
Published: August 2021

Актуальність: необхідність розроблення та впровадження у практику організаційно-педагогічних умов здобуття первинних професійних компетенцій майбутніми судновими механіками у процесі вивчення освітньої компоненти «Морська інженерна практика» визначається вимогами Міжнародної Конвенції «Про підготовку і дипломування моряків та несення вахти 1978 року (з поправками)» (ПДНВ) та базових Модельних курсів ІМО (Course 7.02 «Chief engineer officer and second engineer officer», Course 7.03 Course 7.04 «Officer in charge of an engineering watch»
Мета: обґрунтувати організаційно-педагогічні умови здобуття первинних професійних компетенцій майбутніми судновими механіками при вивченні освітньої компоненти «Морська інженерна практика» та їх вплив на подальшу професійну діяльність у складі екіпажу морського судна.
Методи: теоретичні (логічний аналіз наукових досліджень у галузі професійної педагогіки і методики професійного навчання у вищій школі; логіко-структурний аналіз – для визначення суті й структури понять «професійна компетенція» і «професійна підготовка майбутніх суднових механіків до практичних дій на судні», обґрунтування організаційно-педагогічних умов означеної підготовки); емпіричні (вивчення та аналіз педагогічного досвіду практичної підготовки майбутніх судномеханіків); експериментальні (самооцінювання – для осмислення результатів професійної підготовки до взаємодії у складі екіпажу судна в умовах небезпечних та аварійних ситуацій).
Результати: розроблено та впроваджено в освітній процес організаційно-педагогічні умови формування професійної компетентності у майбутніх суднових механіків, що сприяє побудові у вищому морському закладі освіти ефективної індивідуальної освітньої траєкторії та забезпечує безперервність професійного розвитку майбутніх фахівців.
Висновки: доведено, що впровадження в освітній процес організаційно-педагогічних умов здобуття професійних компетенцій майбутніми судновими механіками у процесі вивчення освітньої компоненти «Морська інженерна практика», надало можливість студентам перейти до освоєння фахових дисциплін на більш високому рівні, про що свідчать дані незалежного тестування. Результативність розроблених організаційно-педагогічних умов підтверджують також результати виконання студентами контрольних робіт на міжкафедральному семінарі.
RCIU severo: A propósito de un caso y revisión de la literatura
Authors: Ayala Yauri Maryllini , Guerrero Torres Jhon Carlo , Ventura Laveriano Walter R. , Sugajara Rosario Ricardo R. , Ibañez Rodríguez Cecilia , Castillo Urquiaga Walter 
Published: August 2021

La restricción del crecimiento intrauterino se define como aquel feto que no alcanza su potencial de crecimiento. Las presentaciones temprana (<32 semanas) y tardía (> 32 semanas) tienen características diferenciales pero no excluyentes; en relación al grado de compromiso obstructivo placentario que repercute en la severidad de las manifestaciones hemodinámicas. Para su diagnóstico, seguimiento y manejo, contamos con la evaluación Doppler multivaso, la cual provee información sobre el deterioro y el riesgo de mortalidad intra útero para establecer el momento oportuno del nacimiento.
Presentamos el reporte de un caso de restricción de crecimiento intrauterino de presentación temprana con alteración doppler severa evaluado por nuestro servicio y revisamos la patogenia, clínica y el enfoque diagnóstico y manejo de esta condición
Caracterización epidemiológica, anticoncepción y bienestar psicológico en mujeres a dos años post aborto terapéutico
Authors: Guevara Ríos Enrique , Carranza-Asmat Cesar , Meza-Santibañez Luis , Ayala-Peralta Félix , Arango-Ochante Pedro , Espinola-Sánchez Marcos , Guevara Beltrán Nora Patricia , Mejía-Muñoz Ana María , Rondón Rondón Marta 
Published: August 2021

Objetivo: Describir las características epidemiológicas, anticoncepción y bienestar psicológico en una serie de mujeres a dos años de post aborto terapéutico. Materiales y métodos: Estudio observacional, descriptivo de una serie de casos. Participaron las mujeres cuyo procedimiento de aborto terapéutico fue realizado en el Instituto Nacional Materno Perinatal del Perú el año 2018 y que al momento de la entrevista hayan transcurrido dos años, previo consentimiento informado. Se recogieron datos sociodemográficos, perfil obstétrico, método de planificación familiar antes del aborto terapéutico y actual. Se aplicó la Escala BIEPS para medir el bienestar psicológico de las participantes a dos años post aborto terapéutico. Se efectuó análisis descriptivo y se salvaguardó la identidad de las participantes. Resultados: Se estudiaron 12 mujeres a dos años de presentar aborto terapéutico. Al momento del aborto terapéutico tenían una edad entre 21 y 38 años, la edad gestacional se encontraba entre 14 y 22 semanas, de los 12 casos cinco mujeres no usaban métodos de anticoncepción. Todos los casos presentaban patología fetal, siendo más frecuente Hidrops fetal e higroma quístico. Todas las mujeres tenían alteración en su salud mental. El procedimiento de aborto más frecuente empleado fue el uso de misoprostol y legrado uterino, en 11 casos. Posterior al aborto terapéutico 7 de 12 no usaron algún método, 5 mujeres presentaron un grado de bienestar psicológico elevado y 7 un nivel alto. En los dominios evaluados de bienestar psicológico los puntajes más altos fueron en propósito en la vida (11 casos de 12), seguido de auto aceptación (10 casos de 12) y crecimiento personal (3 casos de 12). Mientras que, los dominios con una puntuación media fueron relaciones positivas, autonomía y dominio del entorno. Conclusiones: Estos hallazgos sugieren que las mujeres con una gestación con un feto con malformaciones congénitas incompatibles con la vida presentaban una alteración en su salud mental. En estas condiciones, las mujeres presentaron una interrumpen terapéutica de su embarazo; y posteriormente a dos años presentaron un bienestar psicológico adecuado.
Engineering Functional Vasculature in Decellularized Lungs Depends on Comprehensive Endothelial Cell Tropism
Authors: Akinnola Ifeolu , Rossi Daniel R. , Meyer Carolyn , Lindsey Ashley , Haase Douglas R. , Fogas Samuel , Ehrhardt Michael J. , Blue Rachel E. , Price Andrew. P. , Johnson Max , Alvarez Diego F. , Taylor Doris A. , Panoskaltsis-Mortari Angela 
Published: August 2021

Tissue engineering using decellularized whole lungs as matrix scaffolds began as a promise for creating autologous transplantable lungs for patients with end-stage lung disease and can also be used to study strategies for lung regeneration. Vascularization remains a critical component for all solid organ bioengineering, yet there has been limited success in generating functional re-endothelialization of most pulmonary vascular segments. We evaluated recellularization of the blood vessel conduits of acellular mouse scaffolds with highly proliferating, rat pulmonary microvascular endothelial progenitor cells (RMEPCs), pulmonary arterial endothelial cells (PAECs) or microvascular endothelial cells (MVECs). After 8 days of pulsatile perfusion, histological analysis showed that PAECs and MVECs possessed selective tropism for larger vessels or microvasculature, respectively. In contrast, RMEPCs lacked site preference and repopulated all vascular segments. RMEPC-derived endothelium exhibited thrombomodulin activity, expression of junctional genes, ability to synthesize endothelial signaling molecules, and formation of a restrictive barrier. The RMEPC phenotype described here could be useful for identifying endothelial progenitors suitable for efficient vascular organ and tissue engineering, regeneration and repair.
Microbial Warfare on Three Fronts: Mixed Biofilm of Aspergillus fumigatus and Staphylococcus aureus on Primary Cultures of Human Limbo-Corneal Fibroblasts
Authors: Ramírez-Granillo Adrián , Bautista-Hernández Luis Antonio , Bautista-De Lucío Víctor Manuel , Magaña-Guerrero Fátima Sofía , Domínguez-López Alfredo , Córdova-Alcántara Itzel Margarita , Pérez Néstor O. , Martínez-Rivera María de los Angeles , Rodríguez-Tovar Aída Verónica 
Published: August 2021

BackgroundCoinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as Aspergillus fumigatus and Staphylococcus aureus, can form a biofilm. However, mixed fungal–bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.ObjectiveTo determine the capacity of biofilm formation during this fungal–bacterial interaction on primary limbo-corneal fibroblast monolayers.ResultsThe biofilm on the limbo-corneal fibroblast culture was analyzed by assessing biomass production and determining metabolic activity. Furthermore, the mixed biofilm effect on this cell culture was observed with several microscopy techniques. The single and mixed biofilm was higher on the limbo-corneal fibroblast monolayer than on abiotic surfaces. The A. fumigatus biofilm on the human limbo-corneal fibroblast culture showed a considerable decrease compared to the S. aureus biofilm on the limbo-corneal fibroblast monolayer. Moreover, the mixed biofilm had a lower density than that of the single biofilm. Antibiosis between A. fumigatus and S. aureus persisted during the challenge to limbo-corneal fibroblasts, but it seems that the fungus was more effectively inhibited.ConclusionThis is the first report of mixed fungal–bacterial biofilm production and morphological characterization on the limbo-corneal fibroblast monolayer. Three antibiosis behaviors were observed between fungi, bacteria, and limbo-corneal fibroblasts. The mycophagy effect over A. fumigatus by S. aureus was exacerbated on the limbo-corneal fibroblast monolayer. During fungal–bacterial interactions, it appears that limbo-corneal fibroblasts showed some phagocytic activity, demonstrating tripartite relationships during coinfection.
Systematic Comparison of the Performances of De Novo Genome Assemblers for Oxford Nanopore Technology Reads From Piroplasm
Authors: Wang Jinming , Chen Kai , Ren Qiaoyun , Zhang Ying , Liu Junlong , Wang Guangying , Liu Aihong , Li Youquan , Liu Guangyuan , Luo Jianxun , Miao Wei , Xiong Jie , Yin Hong , Guan Guiquan 
Published: August 2021

BackgroundEmerging long reads sequencing technology has greatly changed the landscape of whole-genome sequencing, enabling scientists to contribute to decoding the genetic information of non-model species. The sequences generated by PacBio or Oxford Nanopore Technology (ONT) be assembled de novo before further analyses. Some genome de novo assemblers have been developed to assemble long reads generated by ONT. The performance of these assemblers has not been completely investigated. However, genome assembly is still a challenging task.Methods and ResultsWe systematically evaluated the performance of nine de novo assemblers for ONT on different coverage depth datasets. Several metrics were measured to determine the performance of these tools, including N50 length, sequence coverage, runtime, easy operation, accuracy of genome and genomic completeness in varying depths of coverage. Based on the results of our assessments, the performances of these tools are summarized as follows: 1) Coverage depth has a significant effect on genome quality; 2) The level of contiguity of the assembled genome varies dramatically among different de novo tools; 3) The correctness of an assembled genome is closely related to the completeness of the genome. More than 30× nanopore data can be assembled into a relatively complete genome, the quality of which is highly dependent on the polishing using next generation sequencing data.ConclusionConsidering the results of our investigation, the advantage and disadvantage of each tool are summarized and guidelines of selecting assembly tools are provided under specific conditions.
Analysis of the Clinical Efficacy of Laparoscopy and Hysteroscopy in the Treatment of Tubal-Factor Infertility
Authors: Nian Lei , Yang De-Hong , Zhang Jie , Zhao Han , Zhu Cai-Fen , Dong Ming-Feng , Ai Ying 
Published: August 2021

Objective: This study aims to investigate the clinical efficacy of laparoscopy and hysteroscopy in the treatment of tubal-factor infertility (TFI) to provide a basis for predicting postoperative pregnancy rates.Methods: The clinical data of 336 patients who underwent laparoscopy and hysteroscopy for TFI between February 2018 and December 2018 in the Department of Reproductive Gynecology at the First People's Hospital of Yunnan were retrospectively analyzed. After implementing the inclusion and exclusion criteria, 278 patients were included in the study. The patients were grouped according to pelvic adhesions, hydrosalpinx, twisted fallopian tubes, and fimbriae structure. The impact of the extent of fallopian tube diseases on postoperative pregnancy outcomes was analyzed.Results: Of the 278 patients, 129 got pregnant (pregnancy rate = 46.4%). Pelvic adhesions, hydrosalpinx, twisted/folded fallopian tubes, and damage to the fimbriae of the fallopian tubes were found to affect the natural pregnancy rate after surgery, and it decreased significantly with the aggravation of the disease (P &lt; 0.001). Of the 129 patients who had natural pregnancies, 29 had ectopic pregnancies (ectopic pregnancy rate = 22.48%). Twisted/folded fallopian tubes and damage to the fimbriae structure significantly increased the incidence of postoperative ectopic pregnancy (P &lt; 0.001).Conclusion: Laparoscopy and hysteroscopy are effective treatments for TFI. Pelvic adhesions, twisted/folded fallopian tubes, hydrosalpinx, and damage to the fimbriae of the fallopian tubes can affect postoperative pregnancy outcomes and lead to failure of a natural pregnancy after the operation. The incidence of ectopic pregnancy increases with the degree of fallopian tube twisting/folding and the degree of damage to the fimbriae of the fallopian tubes.
The Spatiotemporal Evolution of MRI-Derived Oxygen Extraction Fraction and Perfusion in Ischemic Stroke
Authors: Wu Di , Zhou Yiran , Cho Junghun , Shen Nanxi , Li Shihui , Qin Yuanyuan , Zhang Guiling , Yan Su , Xie Yan , Zhang Shun , Zhu Wenzhen , Wang Yi 
Published: August 2021

PurposeThis study aimed to assess the spatiotemporal evolution of oxygen extraction fraction (OEF) in ischemic stroke with a newly developed cluster analysis of time evolution (CAT) for a combined quantitative susceptibility mapping and quantitative blood oxygen level-dependent model (QSM + qBOLD, QQ).MethodOne hundred and fifteen patients in different ischemic stroke phases were retrospectively collected for measurement of OEF of the infarcted area defined on diffusion-weighted imaging (DWI). Clinical severity was assessed using the National Institutes of Health Stroke Scale (NIHSS). Of the 115 patients, 11 underwent two longitudinal MRI scans, namely, three-dimensional (3D) multi-echo gradient recalled echo (mGRE) and 3D pseudo-continuous arterial spin labeling (pCASL), to evaluate the reversal region (RR) of the initial diffusion lesion (IDL) that did not overlap with the final infarct (FI). The temporal evolution of OEF and the cerebral blood flow (CBF) in the IDL, the RR, and the FI were assessed.ResultsCompared to the contralateral mirror area, the OEF of the infarcted region was decreased regardless of stroke phases (p &lt; 0.05) and showed a declining tendency from the acute to the chronic phase (p = 0.022). Five of the 11 patients with longitudinal scans showed reversal of the IDL. Relative oxygen extraction fraction (rOEF, compared to the contralateral mirror area) of the RR increased from the first to the second MRI (p = 0.044). CBF was about 1.5-fold higher in the IDL than in the contralateral mirror area in the first MRI. Two patients showed penumbra according to the enlarged FI volume. The rOEF of the penumbra fluctuated around 1.0 at earlier scan times and then decreased, while the CBF decreased continuously.ConclusionThe spatiotemporal evolution of OEF and perfusion in ischemic lesions is heterogeneous, and the CAT-based QQ method is feasible to capture cerebral oxygen metabolic information.
The Extract of Sonneratia apetala Leaves and Branches Ameliorates Hyperuricemia in Mice by Regulating Renal Uric Acid Transporters and Suppressing the Activation of the JAK/STAT Signaling Pathway
Authors: Wu Yu-Lin , Chen Jin-Fen , Jiang Lin-Yun , Wu Xiao-Li , Liu Yu-Hong , Gao Chang-Jun , Wu Yan , Yi Xiao-Qing , Su Zi-Ren , Cai Jian , Chen Jian-Nan 
Published: August 2021

Sonneratia apetala Buch-Ham., an exotic mangrove species with antidiabetic, antibacterial, and antioxidant capacities, mainly distributes in the southeast coastal areas in China. The present work investigated the protective effects of Sonneratia apetala leaves and branches extraction (SAL) on hyperuricemia (HUA) in mice. Potassium oxonate (PO) and hypoxanthine (HX) were used to establish the HUA model by challenge for consecutive 7 days. Results revealed that SAL inhibited the increases in kidney weight and index compared to the vehicle group. Meanwhile, SAL significantly decreased the levels of uric acid (UA), creatinine (CRE), and blood urea nitrogen (BUN) in serum. Additionally, SAL inhibited the activity of xanthine oxidase (XOD) in the liver. SAL ameliorated PO- and HX-induced histopathological changes. Moreover, it regulated oxidative stress markers including malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) activity, and glutathione (GSH) content. Also, SAL inhibited the increases in renal levels of interleukin-6 (IL-6), interleukin-18 (IL-18), interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), monocyte chemotactic protein 1 (MCP-1), and transforming growth factor-β (TGF-β). SAL remarkably reduced suppressor of cytokine signaling 3 (SOCS3), Janus kinase 2 (JAK2), and subsequent phosphorylation of signal transducer and activator of transcription 3 (STAT3) expression. In addition, SAL inhibited the activation of nuclear factor kappa-B (NF-κB) in the kidney. Furthermore, SAL protected against HUA by regulating renal UA transporters of organic anion transporter (OAT1), urate reabsorption transporter 1 (URAT1), and glucose transporter 9 (GLUT9). These findings suggested that SAL ameliorated HUA by inhibiting the production of uric acid and enhancing renal urate excretion, which are related to oxidative stress and inflammation, and the possible molecular mechanisms include its ability to inhibit the JAK/STAT signaling pathway. Thus, SAL might be developed into a promising agent for HUA treatments.
Dihydroartemisinin Alleviates Imiquimod-Induced Psoriasis-like Skin Lesion in Mice Involving Modulation of IL-23/Th17 Axis
Authors: Liu Jiang-Min , Jin Quan-Xin , Fujimoto Manabu , Li Fang-Fang , Jin Lin-Bo , Yu Ran , Yan Guang-Hai , Zhu Lian-Hua , Meng Fan-Ping , Zhang Qing-Gao , Jin Gui-Hua 
Published: August 2021

Background: Psoriasis is a T help 17 (Th17) cell-mediated chronic inflammatory skin disease. Recent studies have shown that dihydroartemisinin (DHA) can significantly reduce experimental autoimmune encephalomyelitis and rheumatoid arthritis by regulating Th17 cells.Objective: To verify whether DHA can improve the symptoms of psoriasis and to further explore the possible mechanism.Methods: The efficiency of DHA was preliminary detected on human keratinocytes (HaCaT) cells in psoriatic condition. Then, imiquimod-induced psoriasis-like model in BALB/c mice was established to evaluate the effects of DHA in vivo.Results: Under the stimulation of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), DHA inhibited the proliferation of HaCaT cells and significantly affected the mRNA expression levels of IFN-γ, interleukin (IL), IL-17A and IL-23. DHA treatment reduced the severity of psoriasis-like skin and resulted in less infiltration of immune cells in skin lesions. DHA restored the expression of IFN-γ, IL-17A, and IL-23 in skins, as well as a decrease of cytokines and chemokines in skin supernatant. DHA also altered the cellular composition in the spleen, which is the makeup of the T cells, dendritic cells (DCs), and macrophages. DHA recovered Th17-related profile with decreased frequency of IL-17+CD4+T cells from splenocyte of mice. Furthermore, DHA also inhibited the concentration of IL-17 from Th17 cells and the expression of Th17 cell-related transcription factors retinoid-related orphan receptor-gamma t (ROR-γt) in vitro. In addition, phosphorylation of signal transducer and activator of transcription-3 (STAT3) was significantly reduced in DHA treatment mice, suggesting that the IL-23/Th17 axis plays a pivotal role.Conclusion: DHA inhibits the progression of psoriasis by regulating IL-23/Th17 axis and is expected to be an effective drug for the treatment of psoriasis.
Normobaric Hypoxia Exposure During Treadmill Aerobic Exercise After Stroke: A Safety and Feasibility Study
Authors: Kelly Liam P. , Basset Fabien Andre , McCarthy Jason , Ploughman Michelle 
Published: August 2021

ObjectiveTo evaluate the safety and feasibility of performing treadmill aerobic exercise in moderate normobaric hypoxia among chronic hemiparetic stroke survivors.DesignObservational study using convenience sampling.SettingResearch laboratory in a tertiary rehabilitation hospital.ParticipantsChronic hemiparetic stroke survivors who could walk at least 10-m with or without assistance and had no absolute contraindications to exercise testing.InterventionParticipants (three male and four female) were asked to complete three normobaric hypoxia exposure protocols within a single session. First, they were passively exposed to normobaric hypoxia through gradual reductions in the fraction of inspired oxygen (FIO2 = 20.9, 17.0, and 15.0%) while seated (5-min at each level of FIO2). Participants were then exposed to the same reductions in FIO2 during constant-load exercise performed on a treadmill at 40% of heart rate reserve. Finally, participants completed 20-min of exercise while intermittently exposed to moderate normobaric hypoxia (5 × 2-min at FIO2 = 15.0%) interspaced with 2-min normoxia intervals (FIO2 = 20.9%).Outcome MeasuresThe primary outcome was occurrence of adverse events, which included standardized criteria for terminating exercise testing, blood oxygen saturation (SpO2) &lt;80%, or acute mountain sickness score &gt;2. The increased cardiovascular strain imposed by normobaric hypoxia exposure at rest and during exercise was evaluated by changes in SpO2, heart rate (HR), blood pressure, and rating of perceived exertion (RPE).ResultsOne participant reported mild symptoms of nausea during exercise in normobaric hypoxia and discontinued participation. No other adverse events were recorded. Intermittent normobaric hypoxia exposure was associated with reduced SpO2 (MD = −7.4%, CI: −9.8 to −5.0) and increased HR (MD = 8.2, CI: 4.6 to 11.7) compared to intervals while breathing typical room air throughout the 20-min constant-load exercise period. The increase in HR was associated with a 10% increase in relative effort. However, reducing FIO2 had little effect on blood pressure and RPE measurements.ConclusionModerate normobaric hypoxia appeared to be a safe and feasible method to increase the cardiovascular strain of submaximal exercise in chronic hemiparetic stroke survivors. Future studies evaluating the effects of pairing normobaric hypoxia exposure with existing therapies on secondary prevention and functional recovery are warranted.