bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uji Aktivitas Antibakteri Kombinasi Minyak Atsiri Biji Ketumbar (Coriandrum sativum L.) dan Gentamisin terhadap Staphylococcus epidermidis
Authors: Triatmoko Bawon , Almuttaqin Huda , Dianasari Dewi 
Published: June 2021

Gentamicin is one of the antibiotics from the aminoglycoside group used for the treatment of infections. Gentamicin has side effect such as permanent ototoxicity and reversible nephrotoxicity. It was necessary to reduce these effects by combining antibiotic with natural ingredients, one of those is coriander seeds essential oil. Combination therapy is done to reduce the potential side effects, and increase antibacterial efficacy. Antibacterial activity test were conducted to measure the MIC values of gentamicin, essential oils of coriander seeds, and combination of both. Based on the antibacterial activity test, MIC value of gentamicin was 4 µg/mL, coriander seed essential oil was 50 µg/mL and MIC combination of gentamicin and essential oil of coriander seeds were 0.5 µg/mL and 50 µg/mL. The FIC Index value obtained was 1.125 meaning that the combination effect was indifferent. Indifferent combination effect means the use of a combination of coriander seeds essential oil and gentamicin as antibacterial was not better than the single use.

Keywords: coriander, essential oil, antibacterial, FIC Index, S. epidermidis
Laser Capture Microdissection (LCM) and 3D Sample Tracking Protocol
Authors: Robbins Shaina , Achanta Sirisha , Vadigepalli Rajanikanth 
Published: June 2021

Abstract
This protocol demonstrates the use of Laser Capture Microdissection (LCM), a precise cell isolation technique, for acquiring single neurons from right atrial ganglionated plexus (RAGP) porcine heart tissue in sync with image acquisition to enable 3D location tracking of collected samples. Fresh frozen RAGP tissue sectioned and stained for neurons (Nissl stain) is processed through LCM for sample collection. The LCM workflow is comprised of four steps: instrument setup, slide loading and tissue inspection, microdissection and image acquisition and sample processing and storage. LCM collected samples were processed for gene expression experiments : High-throughput-qRT-PCR or Single cell RNA sequencing. Gene expression data along with 3D sample tracking was used to generate a functional map of porcine RAGP. The techniques described in this protocol can be adapted to a wide variety of sample and tissue types with minor modifications. This protocol is a part of a protocol pipeline that includes cryosectioning and staining protocols upstream and Tissue Mapper protocol, High-Throughput q-RT-PCR and RNA Seq protocols downstream.
Next Generation Sequencing Based Comprehensive Molecular Analysis in Iranian Patients with Restless Legs Syndrome (RLS)
Authors: ALIBABAEI ZAHRA , Hesampour Ardeshir , Hamdi Seyed.Mohammad.Mehdi , Najafi Mohsen , mohammadpour Hadiseh , Alizadeh Rasoul , Alibabaei Batoul 
Published: June 2021

Abstract
Restless legs syndrome (RLS) is a common sensory-motor neurodegenerative disorder. It usually occurs at night and every 10 to 60 seconds with unpleasant sensations in the legs, burning and itching. Need to move and feel Uncomfortable legs improve with movement.In 20% of patients, symptoms can also include the arm and other parts of the body, which often occur in a more severe form of the disease. But generally, according to research, this syndrome is a multifactorial syndrome. These factors are divided into genetic and environmental or medical factors.In this study, this disorder was investigated genetically and molecularly and the genes affecting the disease in Iranian population were identified by NGS method.Materials and Methods: In this study, NGS and SEQUENCING methods were used to investigate the relationship between genes that influence disease and patients.Conclusion: In this study, we found that ATXN7, MEIS1 and TANC1 genes are effective in restless legs syndrome. In this study, In this study, the effect of TANC gene on the development of restless legs syndrome in Iranian families was confirmed.Discussion: The results of molecular analysis of restless legs syndrome using NGS method led to the identification of new gene loci on ATXN7 and TANC1 genes in Iranian population. Also, gene loci on MEIS1 gene, previous studies on its involvement in restless legs syndrome Confirmed.
The Role of Carbonic Anhydrase Ⅲ and Autophagy in Type 2 Diabetes with Cardio-Cerebrovascular Disease
Authors: Zhang Xiaoming , Tao Ying-Hong , Zhou Xiu-Ling , Shang Xi-Liang , Gong Xiao-Bo , Liu Ying-Chao , Huang Yan-Yan , Chen Gang , Yu Zhong-Yu , Wang Jian-Tao , Du Zun-Guo , Wu Guo-Feng , Zhang Yu , Guo Jing-Chun , Zhou HouGuang 
Published: June 2021

Abstract
BackgroundType 2 diabetes mellitus (T2DM) is one of the most common chronic diseases among the elderly people. The T2DM increases the risk of cardio-cerebrovascular disease (CCD), and the main pathological change of the CCD is atherosclerosis (AS). Meanwhile, the carbonic anhydrases (CAs) are involved in the formation and progression of plaques in AS. However, the exact physiological mechanism of carbonic anhydrase Ⅲ (CAⅢ) has not been clear yet, and there are also no correlation study between CAⅢ protein and T2DM with CCD. MethodsThe 8-week old diabetic mice (db/db−/− mice) and wild-type mice (wt mice) were feed by a normal diet till 32 weeks, and detected the carotid artery vascular opening angle using the method of biomechanics; The changes of cerebral cortex and myocardium were watched by the ultrastructure, and the autophagy were observed by electron microscope; The tissue structure, inflammation and cell injury were observed by Hematoxylin and eosin (HE) staining; The apoptosis of cells were observed by TUNEL staining; The protein levels of CAⅢ, IL-17, p53 were detected by immunohistochemical and Western Blot, and the Beclin-1, LC3, NF-κB were detected by Western Blot. All statistical analysis is performed using PRISM software.ResultsCompared with wt mice, db/db−/− mice’ carotid artery open angle increased significantly. Electron microscope results indicated that autophagy in db/db−/− mice cerebral cortex and heart tissue decreased and intracellular organelle ultrastructure were damaged. HE staining indicated that, db/db−/− mice’ cerebral cortex and heart tissue stained lighter, inflammatory cells infiltration, cell edema were obvious, myocardial fibers were disorder, and myocardial cells showed different degrees of degeneration. Compared with wt mice, TUNEL staining showed that there was obviously increase in db/db−/− mice cortex and heart tissue cell apoptosis. The results of immunohistochemistry and Western Blot indicated that CAⅢ, Beclin-1 and LC3Ⅱ/Ⅰ expression levels conspicuously decreased in cortex and heart tissue of db/db−/− mice, and the expression level of IL-17, NF-κB and p53 obviously increased.ConclusionThere were obvious AS in the carotid artery of db/db−/− mice. And the autophagy participated in the occurrence and development of diabetic CCD. CAⅢ protein might somehow be involved in the regulation of autophagy probably through affecting cell apoptosis and inflammation, but the underlying mechanism remains to be further studied.
Cellulose Nanospheres Coated Polylactic Acid Nonwoven Membranes for Recyclable Use in Oil/Water Separation
Authors: Wen Jiexiu , Yu Qihao , Cui Chaofan , Su Juanjuan , Han Jian 
Published: June 2021

Abstract
Oily wastewater has always been an environmental issue that we are concerned about and committed to managing. Although the superwetting membranes have been extensively studied and widely used in oil/water separation, the abundant discarded membranes are still headaches due to their non-degradable nature. In this work, all-around green, as well as superhydrophilic polylactic acid (PLA) nonwoven membrane, is designed by coating cellulose nanospheres (CNCs) with the aid of polydopamine (PDA). Abundant hydroxyl groups and hierarchical rough structure synergistically contribute to the superhydrophilicity and excellent oil/water separation performance of the resultant CNCs/PDA/PLA nonwoven membrane. Interestingly, the oil/water separation efficiency can be maintained above 98% after 100 times of repeated use. With outstanding durability, and easy controllability, the CNCs/PDA/PLA nonwoven membrane may provide effective solutions to simultaneously manage the oily wastewater and secondary pollution of the used membrane itself.
Negative Cost Girth Problem Using Map-Reduce Framework
Authors: Shamsi Mahboubeh , Kenari Abdolreza Rasouli , Aghamohammadi Roghayeh 
Published: June 2021

Abstract
On a graph with a negative cost cycle, the shortest path is undefined, but the number of edges of the shortest negative cost cycle could be computed. It is called Negative Cost Girth (NCG). The NCG problem is applied in many optimization issues such as scheduling and model verification. The existing polynomial algorithms suffer from high computation and memory consumption. In this paper, a powerful Map-Reduce framework implemented to find the NCG of a graph. The proposed algorithm runs in O(log k) parallel time over O(n3) on each Hadoop nodes, where n; k are the size of the graph and the value of NCG, respectively. The Hadoop implementation of the algorithm shows that the total execution time is reduced by 50% compared with polynomial algorithms, especially in large networks concerning increasing the numbers of Hadoop nodes. The result proves the efficiency of the approach for solving the NCG problem to process big data in a parallel and distributed way.
Oxaliplatin Compromised CDK1 Activity Sensitizes BRCA-Proficient Cancers to PARP Inhibition in Oxaliplatin Resistance Gastric Cancer
Authors: Li Huafu , Wang Chunming , Lan Linxiang , Wu Wenhui , Evans Ian , Ruiz E Josue , Yan Leping , Zhang Haiyong , Zhou Zhijun , Hu Zhenran , Chen Wei , Behrens Axel , He Yulong , Zhang Changhua 
Published: June 2021

Abstract
Background: Oxaliplatin resistance is one of the most important problems in the treatment of cancer. The successful culture of tumor organoid in gastric cancer can help us to study oxaliplatin resistance and its mechanism. Thus, it is convenient for us to successfully solve oxaliplatin resistance and improve the prognosis of patients.Methods: Two oxaliplatin resistant patients and two oxaliplatin sensitive patients were enrolled through our Gastric Cancer Center of Sun Yat-sen University. Core genes of oxaliplatin resistant and non-resistant patients were analyzed by sequencing. The overexpression and knockdown of core genes were carried out by organoid in vivo, combined with oxaliplatin-resistant cell lines AGS, MKN74 and SNU719 for cell viability, WB and immunofluorescence, etc., to verify the role of core genes in oxaliplatin resistance. Again, in vivo experiments were verified by subcutaneous tumor formation in vitro.Results: Through sequencing, we found that PARP1 is an important core gene leading to oxaliplatin resistance. In vivo organoids, oxaliplatin resistant cell lines and subcutaneous tumor formation in vivo. We found that PARP1 was an important cause of oxaliplatin resistance. Oxaliplatin can inhibit CDK1 activity and make cancer with normal BRCA1 function sensitive to PARP inhibition. Through the combination of oxaliplatin and PARP1 inhibitor olaparib, we can effectively kill tumor cells. Through the patients' follow-up data, we found that the expression level of PARP1 was significantly correlated with oxaliplatin resistance.Conclusion: Our results indicate that PARP1 is an important core gene leading to oxaliplatin resistance. Combined oxaliplatin and PARP1 inhibitor olaparib can effectively kill tumor cells. Oxaliplatin can inhibit CDK1 activity and make cancers with normal BRCA1 function more sensitive to PARP inhibitors.
Phytotoxic Effect Of Aqueous Extracts of Different Plant Parts Of Sphaeranthus Indicus On Germination And Seedling Growth Of Eleasine Coracana
Authors: Kumbhar B. A. , Dabgar P. J. 
Published: June 2021

Abstract
BackgroundThe phytotoxic influences of water extract of different plant parts of Sphaeranthus indicus (Burandu) were tested by growing test crop of Eleasine coracana (Nagli) for seedling growth. Significant differences in the growth of the test crop were observed. ResultThe hypocotyl growth of test crop depressed significantly at high concentration (6%) aqueous extract of different plant parts of S. indicus was found after 10 days. Redicle length of Nagli crop was stimulated in 2% shoot aqueous extract of Sphaeranthus indicus whereas other concentrations of all aqueous extracts of Burandu weed show phytotoxic effects as compared to control after ten days. Shoot and Root dry weights of food crop were reduced by Burandu residue. ConclusionTest crop Nagli was more sensitive to root aqueous extract while less sensitive to shoot aqueous extract of Sphaeranthus indicus.
Long-Term Adverse Effects of Cigarette Smoking on the Incidence Risk of Metabolic Syndrome: Longitudinal Findings of the Korean Genome and Epidemiology Study Over 12 Years
Authors: Kim Ae Hee , Seo In-Ho , Lee Hye Sun , Lee Yong-Jae 
Published: June 2021

Abstract
BackgroundPrevious studies have demonstrated positive associations between smoking and metabolic syndrome (MetS) in several cross-sectional studies. However, the association was not consistent in long-term studies, which makes longitudinal effects remain controversial. Thus, we investigated the association between cigarette smoking and incidence risk of MetS in a large-sample, community-based, longitudinal prospective study in both intensity and cumulative dose of cigarette smoking over 12 years of follow-up. MethodsAmong 10,038 participants, a total of 5568 men ages 40-69 years old without MetS at baseline were selected from the Korean Genome and Epidemiology Study (KoGES). The hazard ratios (HRs) and 95% confidence interval (CIs) for incident MetS were calculated using a multivariate Cox proportional-hazards regression model after adjusting for potential confounding variables and setting never smokers as the reference group for intensity (expressed as number of cigarettes per day) and duration of smoking (expressed as number of pack-years). Results Compared to the referent never smokers, the HRs (95% CIs) for incident MetS increased as the intensity of smoking in current smokers also increased: 1.50 (1.07-2.01) for 0-9 cigarettes/day, 1.66 (1.34-2.06) for 10-19 cigarettes/day, and 1.75 (1.34-2.29) for ≥20 cigarettes/day, after adjusting for age, alcohol drinking, physical activity, household income, educational level, mean arterial pressure, triglyceride, HDL-cholesterol level, and homeostasis model assessment insulin resistance. These positive relationships were similar when the cumulative dose of smoking was used in current smokers. Compared to the referent never smokers, the HRs (95% CIs) for incident MetS increased as the cumulative dose also increased: 1.63 (1.32-2.02) for <20 PYs and 1.67 (1.30-2.14) for 20 ≥PYs after adjusting for the same co-variables. ConclusionsBoth cigarette smoking intensity and cumulative dose were positively associated with MetS among community-dwelling Korean men in the large-scale, longitudinal, prospective, 12-year follow-up study.
Litter inputs and phosphatase activity control the temporal variability of organic phosphorus in a tropical forest soil in the Central Amazon
Authors: Schaap Karst J. , Fuchslueger Lucia , Hoosbeek Marcel R. , Hofhansl Florian , Martins Nathielly Pires , Valverde-Barrantes Oscar J. , Hartley Iain P. , Lugli Laynara F. , Quesada Carlos Alberto 
Published: June 2021

Abstract
Purpose. The tropical phosphorus-cycle and its impacts on phosphorus (P) availability are a major uncertainty in projections of forest productivity. In highly weathered soils with low P concentrations, plant and microbial communities depend on biological and physical processes to acquire P. We explored the seasonality and relative importance of drivers controlling the fluctuation of common P pools via processes such as litter production and decomposition, and soil phosphatase activity. Methods. We analyzed variation of standard tropical soil phosphorus pools over one year. In addition, we measured litterfall, its decomposition rates and soil extracellular phosphatase enzyme activity and tested their relation to the fluctuations in P-fractions. Results. Our results show clear patterns of seasonal variability of soil P fractions during the year. We found that modeled P released during litter decomposition is positively related to change in organic P fractions, while net change in organic P fractions was negatively related to phosphatase activities in the top 5 cm. Conclusion. We conclude that input of organic P forms by litter decomposition and organically produced phosphatases are the two main factors controlling seasonal soil P fluctuation, and therefore the P economy in P impoverished soils. Organic soil P follows a clear seasonal pattern, indicating tight cycling of the nutrient, while reinforcing the importance of studying soil P as an integrated dynamic system in a tropical forest context.
Ultrafast Synthesis of MoS2 Quantum dot Particles using Low Energy at Room Temperature
Authors: Wu Jun Yi 
Published: June 2021

Abstract
In this study, a new method of synthesizing large amount highly fluorescent MoS2 quantum dots is presented. This low environmental impact ,ultrafast ,saving energy method can synthesize a large number of MoS2 quantum dots(QDs). Using the NaCl crystal particles sharp edge that are insoluble in the supersaturated NaCl solution combined with the shear force generated by the press shaker to cut the MoS2 bulk material to form MoS2 QDs.Large amount highly fluorescent MoS2 quantum dots in a matter of 3 min using the NaCl crystal as a sustainable cutting agent.The prepared MoS2 QDs were characterized by TEM ,FESEM ,EDX ,DLS ,XPS, UV-vis Spectroscopy ,Florescence Spectroscopy analyses. Furthermore, this ultrafast, low energy consumption,green process may offer a potential major advance for production of other QDs materials from bulk material.
Is a Stop Solution Necessary for Metal Nanoparticles Aggregation-based Colorimetric Assays?
Authors: Jouyban Abolghasem , samadi azam 
Published: June 2021

Abstract
We report herein for the first time that a stop solution should be used to terminate the metal nanoparticles aggregation reactions especially salt-induced ones to increase the repeatability of the assays. It was found that hydrophobic surfactants have the ability to slow down the aggregation process of MNPs and can be applied as stop solution for aggregation based colorimetric assays. This trick provides accurate and well-repeatable signals since the aggregation of the nanoparticles is a dynamic process and capturing signal in a predetermined time is difficult.
Aquatic Macrophytes in Southern Amazonia, Brazil: Richness, Endemism, and Comparative Floristics
Authors: Neyra Milton Omar Cordova , Keffer Josiane Fernandes , Giacoppini Dienefe Rafaela , Pott Vali Joana , Pott Arnildo , Munhoz Cássia Beatriz Rodrigues 
Published: June 2021

Abstract
Southern Amazonia potentially harbors a wide diversity of aquatic macrophyte species because of its diverse wetland habitats and location in the Amazon-Cerrado transition zone, the two largest biogeographic domains in South America. We inventoried the taxonomic diversity of aquatic macrophytes in the region, including endemism, habitats, species life forms, and floristic comparisons with other Brazilian regions. We found a high number of species of aquatic macrophytes for the southern Amazon region, comparable to extensive inventories in larger areas in southern, northeastern, and northern regions of Brazil. We recorded 688 species of aquatic macrophytes in 299 genera and 95 families, of which 66 species are new records, 89 endemic, and 5 exclusive to Brazilian Amazonia. We present new records of aquatic macrophyte species, together with their degree of endemism and occurrence restricted to the region. The macrophyte species list of southern Amazonia showed 60% similarity to inventories in the Xingu basin and in northern Brazil. This high diversity of aquatic macrophytes in the Southern Amazonia, with endemic species and others with restricted ranges, emphasizes the importance of conserving wetlands and vegetation types where they grow in the region.
Dietary Carbohydrate Intake and New-onset Diabetes: A Nationwide Cohort Study in China
Authors: Zhou Chun , Zhang Zhuxian , Liu Mengyi , Zhang Yuanyuan , Li Huan , He Panpan , Li Qinqin , Liu Chengzhang , Qin Xianhui 
Published: June 2021

Abstract
Background: The association of carbohydrate intake with diabetes risk remains uncertain. We aimed to evaluate the prospective associations of the amount and types of carbohydrate intake with new-onset diabetes.Methods: A total of 16,260 non-diabetic participants from the China Health and Nutrition Survey (CHNS) were included. Dietary intake was collected by three consecutive 24-h dietary recalls combined with a household food inventory. Participants with self-reported diabetes, or fasting plasma glucose ≥7.0 mmol/L or glycated hemoglobin ≥6.5% during the follow-up were defined having new-onset diabetes. Results: During a median follow-up of 9 years (158,930 person-years), 1,100 participants developed diabetes. Overall, there was a U-shaped association between percent of energy from carbohydrate intake and new-onset diabetes, with minimal risk at 49-56% of energy from total carbohydrate intake (quartile 2) (P for nonlinearity <0.001). Moreover, there was an L-shaped association between high-quality carbohydrate intake and new-onset diabetes (P for nonlinearity <0.001), and a J-shaped association of low-quality carbohydrate intake with new-onset diabetes (P for nonlinearity <0.001). Furthermore, there was an inverse association between the plant-based low-carbohydrate scores for low-quality carbohydrate and new-onset diabetes. However, a reversed J-shaped association was found between the animal-based low-carbohydrate scores for low-quality carbohydrate and new-onset hypertension (P for nonlinearity <0.001). Conclusions: There was a U-shape association between percent of total carbohydrate intake and new-onset diabetes, with the lowest risk at 49-56% carbohydrate intake. Our findings provide some evidence for the intake of high-quality carbohydrate, and the substitution of plant-based products for low-quality carbohydrate for primary prevention of diabetes.