bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guideline on current good radiopharmacy practice (cGRPP) for the small-scale preparation of radiopharmaceuticals
Authors: Gillings Nic , Hjelstuen Olaug , Ballinger Jim , Behe Martin , Decristoforo Clemens , Elsinga Philip , Ferrari Valentina , Peitl Petra Kolenc , Koziorowski Jacek , Laverman Peter , Mindt Thomas L. , Neels Oliver , Ocak Meltem , Patt Marianne , Todde Sergio 
Published: February 2021

AbstractThis guideline on current good radiopharmacy practice (cGRPP) for small-scale preparation of radiopharmaceuticals represents the view of the Radiopharmacy Committee of the European Association of Nuclear Medicine (EANM). The guideline is laid out in the format of the EU Good Manufacturing Practice (GMP) guidelines as defined in EudraLex volume 4. It is intended for non-commercial sites such as hospital radiopharmacies, nuclear medicine departments, research PET centres and in general any healthcare establishments. In the first section, general aspects which are applicable to all levels of operations are discussed. The second section discusses the preparation of small-scale radiopharmaceuticals (SSRP) using licensed generators and kits. Finally, the third section goes into the more complex preparation of SSRP from non-licensed starting materials, often requiring a purification step and sterile filtration. The intention is that the guideline will assist radiopharmacies in the preparation of diagnostic and therapeutic SSRP’s safe for human administration.
Potential contributors to low dose methotrexate toxicity in a patient with rheumatoid arthritis and pernicious anemia: case report
Authors: Jara-Palacios Miguel A. , Chun William , Traub Nomi L. 
Published: February 2021

AbstractBackgroundLow dose methotrexate toxicity rarely occurs, but may present with severe complications, such as pancytopenia, hepatotoxicity, mucositis, and pneumonitis. Known risk factors for methotrexate toxicity include dosing errors, metabolic syndrome, hypoalbuminemia, renal dysfunction, lack of folate supplementation, and the concomitant use of drugs that interfere with methotrexate metabolism. Vitamin B12 deficiency leads to megaloblastic anemia and may cause pancytopenia, but its role in methotrexate toxicity has not been described.Case presentationWe present a case of a patient with rheumatoid arthritis who was admitted with febrile neutropenia, pancytopenia, and severe mucositis, likely secondary to low dose methotrexate toxicity. She had multiple factors that potentially contributed to the development of toxicity, including concurrent sulfasalazine use for rheumatoid arthritis. An evaluation of the patient’s macrocytic anemia revealed pernicious anemia. The patient’s illness resolved with cessation of methotrexate and sulfasalazine, leucovorin treatment and vitamin B12 repletion.ConclusionsThis case illustrates the multiple factors that may potentially contribute to low dose methotrexate toxicity and highlights the importance of testing for vitamin B12 deficiency in rheumatoid arthritis patients with macrocytic anemia. Addressing all the modifiable factors that potentially contribute to low dose methotrexate toxicity may improve outcomes.
Endophytic bacteria isolated from higher plant in Aceh, Indonesia, and their chemical compounds activity against Fusarium oxysporum f. sp. lycopersici
Authors: Maulidia Vina , Sriwati Rina , Soesanto Loekas , Syamsuddin  , Hamaguchi Takahiro , Hasegawa Koichi 
Published: February 2021

Abstract
Background
Endophytic bacteria are an association between bacteria and plant tissue that could play a role as a biocontrol agent.

Main body
Endophytic bacteria were isolated from several high root plants in Aceh, Indonesia. This study aimed to detect the chemical compounds of the potential endophytic bacteria as a biocontrol agent against Fusarium oxysporum f. sp. lycopersici (FOL). There were 198 endophytic bacterial isolates detected in roots of 9 higher plant. The hypersensitive reaction showed that 193 isolated endophytic bacteria were non-pathogenic. There were 13 isolated endophytic bacteria that worked to inhibit FOL between 50.0 and 89.2%; such endophytic bacteria were isolated from Solanum lycopersicum L., Psidium guajava L., Dendrocalamus asper (Schult with f.) Backer ex Heyne, Pinus merkusii L., Theobroma cacao L., and Albizia chinensis L. Molecular identification using 16S rRNA gene sequence confirmed that the endophytic bacteria were derived from species Pseudomonas aeruginosa, P. mosselii, Arthrobacter sp., Bacillus cereus, B. thuringiensis, and Serratia marcescens. P. aeruginosa that showed the highest inhibition was analyzed using GC-MS analysis. The analysis identified that antibiotics as Pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- was produced by P. aeruginosa succeeded in suppressing FOL.

Conclusion
The study recommends the species P. aeruginosa, as effective endophytic bacteria for the control of FOL pathogen.
Perspectives on urban transformation research: transformations in, of, and by cities
Authors: Hölscher Katharina , Frantzeskaki Niki 
Published: February 2021

AbstractThe narrative of ‘urban transformations’ epitomises the hope that cities provide rich opportunities for contributing to local and global sustainability and resilience. Urban transformation research is developing a rich yet consistent research agenda, offering opportunities for integrating multiple perspectives and disciplines concerned with radical change towards desirable urban systems. We outline three perspectives on urban transformationsin,ofandbycities as a structuring approach for integrating knowledge about urban transformations. We illustrate how each perspective helps detangle different questions about urban transformations while also raising awareness about their limitations. Each perspective brings distinct insights about urban transformations to ultimately support research and practice on transformationsforsustainability and resilience. Future research should endeavour to bridge across the three perspectives to address their respective limitations.
Ultraviolet spectrophotometric methods for the determination of the minor component presented in fixed-dose pharmaceutical combinations through the last two decades (2000–2020)
Authors: Obaydo Reem H. , Al Zakri Duaa J. , Sakur Amir Alhaj , Lotfy Hayam M. 
Published: February 2021

AbstractBackgroundThe pharmaceutical industry and the National Regulatory Authorities are now focusing on the quantification of multi-component drugs for quality control testing.Main bodyRecently, the utilization of the ultraviolet spectrophotometric methods has become vital for the analytical studies for the routine analysis of different fixed-dose combination products either in the presence of a minor component in their combination or not. This article reviewed several published methods to those that have been applied to quantify some pharmaceutical combinations containing minor components through the last two decades.ConclusionThe applied spectrophotometric methods are labor, time-saving, and also considered a cheap substitute for the overpriced high-performance liquid chromatographic technique.
Transformed notochordal cells trigger chronic wounds in zebrafish, destabilizing the vertebral column and bone homeostasis
Authors: López-Cuevas Paco , Deane Luke , Yang Yushi , Hammond Chrissy L. , Kague Erika 
Published: February 2021

ABSTRACT
Notochordal cells play a pivotal role in vertebral column patterning, contributing to the formation of the inner architecture of intervertebral discs (IVDs). Their disappearance during development has been associated with reduced repair capacity and IVD degeneration. Notochord cells can give rise to chordomas, a highly invasive bone cancer associated with late diagnosis. Understanding the impact of neoplastic cells during development and on the surrounding vertebral column could open avenues for earlier intervention and therapeutics. We investigated the impact of transformed notochord cells in the zebrafish skeleton using a line expressing RAS in the notochord under the control of the kita promoter, with the advantage of adulthood endurance. Transformed cells caused damage in the notochord and destabilised the sheath layer, triggering a wound repair mechanism, with enrolment of sheath cells (col9a2+) and expression of wt1b, similar to induced notochord wounds. Moreover, increased recruitment of neutrophils and macrophages, displaying abnormal behaviour in proximity to the notochord sheath and transformed cells, supported parallels between chordomas, wound and inflammation. Cancerous notochordal cells interfere with differentiation of sheath cells to form chordacentra domains, leading to fusions and vertebral clefts during development. Adults displayed IVD irregularities reminiscent of degeneration, including reduced bone mineral density and increased osteoclast activity, along with disorganised osteoblasts and collagen, indicating impaired bone homeostasis. By depleting inflammatory cells, we abrogated chordoma development and rescued the skeletal features of the vertebral column. Therefore, we showed that transformed notochord cells alter the skeleton during life, causing a wound-like phenotype and activating chronic wound response, suggesting parallels between chordoma, wound, IVD degeneration and inflammation, highlighting inflammation as a promising target for future therapeutics.
This article has an associated First Person interview with the first author of the paper.
Abstrak Konggres dan Seminar Nasional Penggalang Taksonomi Tumbuhan Indonesia (PTTI) Universitas Sebelas Maret Surakarta, 19-20 Desember 2003
Authors: BIODIVERSITAS SISIPAN 
Published: February 2021

Sonneratia spp. was genetically unique genera. They had some members in Java, especially Sonneratia alba and S. caseolaris. This species have ability to cross reproduction, and produce fertile hybrid, although the fertility is lower than the parent, so they hard to differÂ morphologically. This research had been done in 6 sites (populations) of the southern and northern coast of Java, i.e. Bogowonto estuary, Kulonprogo (1 pop.), Segara Anakan, Cilacap (2 pops), coast of Pasar Bangi, Rembang (1pop), Juwana estuary, Pati (1 pop), and Canal Wulan estuary, Demak (1 pop). The research indicated that S. alba was more dominant, but there were some confused inmorphological character, that may be caused by hybridization. Isozymic analyses on peroxidase and esterase marker to 60 individuals (10 per population) had been no differing in isozymic band significantly. They have similarity index value more than 60%. It indicated, that they tend to same species than separate it.Keywords: isozyme, diversity, Sonneratia, northern andsouthern coast of Java.
Population dynamics of dominant demersal fishes caught in Tambelan Islands waters, Riau Archipelago Province
Authors: Phdmrk@gmail.com YOVITNER , FAHMI FAHMI 
Published: February 2021

Yonvitner, Fahmi. 2012. Population dynamics of dominant demersal fishes caught in Tambelan Islands waters, Riau ArchipelagoÂ Province. Biodiversitas 14: 200-204. The population of demersal fishes tends to be decline in the last few decades. The decline was dueÂ to the increased of intensive catches, fishing effort and illegal fishing practices. This study aimed to examine aspects of populationÂ dynamics such as mortality, recruitment, opportunity capture and population estimation. The study was conducted in six locations atÂ Tambelan waters, on 4-16 November 2010. Collected data included the fish species, size and biomass catch. The analyses performedÂ included mortality, recruitment, opportunity capture and population estimates (VPA). The data indicated that the Genus of Nemipterus and Lutjanus possess the highest total mortality rate that was respectively 1.62 and 2.55. The highest recruitment presentation of the population which has an annual period as Pentapodus was 16%. Catch opportunities tend to be higher than actual fishing, while the alleged biomass of steady state conditions reached 12.2 tons/yr.
Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
Authors: Ratnasari Jujun , Esyanti Rizkita Rachmi , Tan Marselina Irasonia , Juliawaty Lia Dewi , Shimma Shuichi 
Published: February 2021

Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with α-cyano-4-hydroxycinnamic acid (α-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.
Combination on endophytic fungal as the Plant Growth-Promoting Fungi (PGPF) on cucumber (Cucumis sativus)
Authors: Syamsia Syamsia , IDHAN ABUBAKAR , FIRMANSYAH AMANDA PATAPPARI , NOERFITRYANI NOERFITRYANI , RAHIM IRADHATULLAH , KESAULYA HENRY , ARMUS RAKHMAD 
Published: February 2021

Abstract. Syamsia S, Idhan A, Firmansyah AP, Noerfitryani N, Rahim I, Kesaulya H, Armus R. 2021. Combination on endophytic fungal as the Plant Growth-Promoting Fungi (PGPF) on Cucumber (Cucumis sativus). Biodiversitas 22: 1194-1202. Endophytic fungi are known to stimulate plant growth by producing secondary metabolites, including phytohormones (IAA and Gibberellins), siderophore, phosphate-solubilizing metabolites. In this study, a total of six endophytic fungi were successfully isolated from local rice plants and showed different abilities in producing secondary metabolites, during single isolates testing. These six isolates were then combined to obtain 15 combinations for analysis, to determine the best combination for application as a plant growth promoter. Subsequently, each combination was tested for phytohormones (IAA, gibberellins) and siderophore (quantitatively)-producing activity, phosphate-solubilizing ability, and the effect on cucumber (Cucumis sativus L) plant growth. F13 showed activity in producing IAA and produced the highest gibberellin levels, while F1 exhibited the highest phosphate-solubilizing activity. In addition, F11 (Na-salicylate) and F1 (catechol) showed the highest siderophore activity, while a combination of F6, F8, F9, and F12 successfully increased plant height growth. Also, F4 increased the root growth, while the fresh weight of cucumber was increased by F8 treatment, under controlled conditions. Molecular analysis showed the tested isolates have close similarity to Daldinia eschscholtzii, Sarocladium oryzae, Rhizoctonia oryzae, Penicillium allahabadense, and Aspergillus foetidus. The combination of endophyte fungal isolates showed potential as plant growth promoters, however, further testing on several plant types is required before the combination is to be widely applied.
2.05-µm all-fiber laser source designed for CO2and wind coherent lidar measurement
Authors: Lahyani Julien , Le Gouët Julien , Gibert Fabien , Cézard Nicolas 
Published: February 2021

This work reports on an all-fiber pulsed laser source for simultaneous remote sensing ofCO2concentration and wind velocity in the 2.05 µm region. The source is based on a polarization-maintaining master oscillator power amplifier (MOPA) architecture. Two narrow-linewidth master oscillators for ON-line/OFF-lineCO2differential absorption lidar operation alternately seed a four-stage amplifier chain at a fast switching rate up to 20 kHz. The MOPA architecture delivers laser pulses of 120 µJ energy, 200 ns duration (600 W peak power) at 20 kHz pulse repetition rate (2.4 W average power). The output linewidth is lower than 5 MHz, close to the pulse Fourier transform limit, and the beam quality factor isM2=1.12. The source also provides a pre-amplified 20 mW local oscillator with a relative intensity noise of−160dB/Hzthat ensures optimal performance for future coherent detection.
Ambulant sale of food in the street nearby events: the issue of food (in)security from the perspective of the consuming public
Authors: Melo Diêgo Fernandes de , Pinto Rafaela Caetano 
Published: February 2021

This article discusses issues related to the practice of street commerce near to events that occur in the Distrito Federal (DF), from the consumers' point of view. In order to do this, with the help of aÂ survey, we sought to collect the information about the public's perceptions and consumption habits, to identify the elements that interfere in the option for consumption inside the event or with street vendors and how consumers evaluate the problem of food security in the provision of this service. The results demonstrate that 48.94% of consumers have a habit of consuming food sold by street vendors and that only 14.89% have knowledge about the rules of good practice in food handling and consider them when selecting consumption places of edibles.
Durability and stability of antimicrobial coated surfaces
Authors: Buhl Sebastian , Peter J. , Stich A. , Brückner R. , Bulitta C. 
Published: February 2021

Abstract
Antimicrobial surface coating of i.e. medical devices could contribute to infection prevention and reduction of hospital acquired infections (HAI). Recent studies showed a significant reduction in the microbial contamination of antimicrobial coated surfaces in clinical setups. Nevertheless, there are only few publications available that deal with the durability and stability of these coatings under routine clinical conditions. In this work different antimicrobial coating compositions were tested on different surfaces for their durability and remaining antimicrobial activity. Our results show that the durability and stability of a subsequent applied antimicrobial coating is strongly dependent on the chemical formulation of the coating and also the underlying surface condition. Whereas we could still detect remaining antimicrobial coating and activity on some samples after repeated abrasion testing, some other samples lost their coating and activity after only a few abrasion cycles. Interestingly the integrated antimicrobial substance in the 3-D printed samples showed strong antimicrobial activity even after rough treatment of the surfaces (brushing, scratching).
Investigating an Optimal Signal Epoch Length for Cardiotocographic Classification
Authors: Fuentealba Patricio , Illanes Alfredo , Ortmeier Frank , Poudel Prabal 
Published: February 2021

Abstract
This work focuses on investigating an optimal foetal heart rate (FHR) signal segment to be considered for automatic cardiotocographic (CTG) classification. The main idea is to evaluate a set of signal segments of different length and location based on their classification performance. For this purpose, we employ a feature extraction operation based on two signal processing techniques, such as the Improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise and time-varying autoregressive modelling. For each studied segment, the features are extracted and evaluated based on their performance in CTG classification. For the proposed evaluation, we make use of real CTG data extracted from the CTU-UHB database. Results show that the classification performance depends considerably on the selected FHR segment. Likewise, we have found that an optimal FHR segment for foetal welfare assessment during labour corresponds to a segment of 30 minutes long.
Fifth DSRG symposium at CHU UCL Namur, 18/10/2019. “Centralization of injectables and robotization”
Authors: Hecq Jean-Daniel , Jamart Jacques , Odou Pascal , Vigneron Jean , Galanti Laurence 
Published: February 2021

Abstract
The physico-chemical stability of an injectable preparation (IV) is conditioned by different parameters. A collaboration between the pharmacy, the chemistry laboratory and the statisticians of the scientific support unit was established in 1996, in order to carry out long-term chemical stability studies of commonly used IVs and to be able to take charge of their preparation in pharmacy. In 24 years of activity, the Drug Stability Research Group (DSRG) tested 39 IV at different concentration and temperature of storage. The DSRG has organized an annual symposium since 2015. The theme of the 2019 edition was devoted to the robotization of injectable reconstitution operations, focused on their impact on the workplace and the existing equipment.
“Big girls don’t cry”: the effect of the experimenter’s sex and pain catastrophising on pain
Authors: Vo Lechi , Drummond Peter D. 
Published: February 2021

Abstract

Objectives
The expression of pain in males and females involves complex socio-psychological mechanisms. Males may report lower pain to a female experimenter to appear strong, whereas females may report higher pain to a male experimenter to appear weak and to seek protection. However, evidence to support these stereotypes is inconclusive. Individuals who catastrophise about pain rate higher pain than those who do not. How pain catastrophising interacts with the effect of the experimenter’s sex on pain reports is yet to be explored. Thus, the aim of this study was to determine whether pain catastrophising moderated the effect of the experimenter’s sex on pain reports in healthy males and females.

Methods
Participants (n=60, 30 males) were assigned to one of four experimental conditions: males tested by male experimenters, males tested by female experimenters, females tested by male experimenters, and females tested by female experimenters. Participants completed the Pain Catastrophising Scale, and then sensitivity to heat and to blunt (pressure-pain threshold) and sharp stimuli was assessed on both forearms, and to high frequency electrical stimulation (HFS) administered to one forearm.

Results
Females reported lower pressure-pain thresholds than males irrespective of the experimenters’ sex. Females reported lower sharpness ratings to male than female experimenters only when the test stimuli were moderately or intensely sharp. Higher pain catastrophising scores were associated with higher sharpness ratings in females but not males. Additionally, higher pain catastrophising scores were associated with greater temporal summation of pain to HFS, and with lower pressure-pain thresholds in females who were tested by male experimenters.

Conclusions
These findings indicate that the experimenters’ sex and the participant’s pain catastrophising score influence pain reports, particularly in females. Awareness of these psychosocial factors is important in order to interpret pain responses in a meaningful way, especially when females are tested by male experimenters. A greater awareness of sex/gender role biases and their potential interaction with pain catastrophising may help researchers and clinicians to interpret pain reports in meaningful ways. In turn, this may help to improve delivery of treatments for patients with chronic pain.