bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA‐seq analysis of laser microdissected Arabidopsis thaliana leaf epidermis, mesophyll and vasculature defines tissue‐specific transcriptional responses to multiple stress treatments
Authors: Berkowitz Oliver , Xu Yue , Liew Lim Chee , Wang Yan , Zhu Yanqiao , Hurgobin Bhavna , Lewsey Mathew G. , Whelan James 
Published: May 2021

SUMMARY
Acclimation of plants to adverse conditions requires the coordination of gene expression and signalling pathways between tissues and cell types. As the energy and carbon capturing organs, leaves are significantly affected by abiotic and biotic stresses. However, tissue‐ or cell type‐specific analyses of stress responses have focussed on the Arabidopsis root. Here, we comparatively explore the transcriptomes of three leaf tissues (epidermis, mesophyll, vasculature) after induction of diverse stress pathways by chemical stimuli (antimycin A, 3‐amino‐1,2,4‐triazole, methyl viologen, salicylic acid) and ultraviolet light in Arabidopsis using laser capture microdissection followed by RNA sequencing. Stimulation of stress pathways caused an overall reduction in the number of genes expressed in a tissue‐specific manner, though a small subset gained or changed their tissue specificity. We find no evidence of a common stress response, with only a few genes consistently responsive to two or more treatments in the analysed tissues. However, differentially expressed genes overlap between tissues for individual treatments. A focussed analysis provided evidence for an interaction of auxin and ethylene that mediates retrograde signalling during mitochondrial dysfunction specifically in the epidermis, and a gene regulatory network defined the hierarchy of interactions. Taken together, we have generated an extensive reference dataset that will be valuable for future experiments analysing transcriptional responses on a tissue or single‐cell level. Our results will enable the tailoring of the tissue‐specific engineering of stress‐tolerant plants.
A New Heat Source Model for Keyhole Mode Laser Welding
Authors: Lorin Samuel , Madrid Julia , Söderberg Rikard , Wärmefjord Kristina 
Published: May 2021

Abstract
Laser welding is a common technique for joining metals in many manufacturing industries. During welding, a weld gun traverses the interface of the parts to be joined causing them to melt, fuse, and solidify when the temperature decreases, thus joining the parts. Due to the heat input and the resulting melting and solidification, the parts deform causing residual distortion and residual stresses. To assure the geometrical and functional quality of the product, computational welding mechanics (CWM) is often employed in the design phase to predict the outcome of different design proposals. Furthermore, CWM can be used to design the welding process with the objective of assuring the quality of the weld. However, welding is a complex multiphysical process including the weld pool flow, microstructure dynamics, and structural mechanics. In a design process, it is typically not feasible, for example, to employ fluid simulation of the weld pool in order to predict deformation of a welded assembly, especially if a set of design proposals is under investigation. This is because of the high resolution needed for these fluid simulations in combination with challenges to couple fluid simulation with structural simulation. Instead, what is used is a heat source that emulates the heat input from the melt pool. An example of a heat source is the standard doubled ellipsoid. This heat source has been efficiently used for a large number of welding simulation. However, standard heat sources are typically not flexible enough to capture the fusion zone for deep keyhole mode laser welding. In this study, we presented a new heat source model for keyhole mode laser welding. In an industrial case study, a number of bead-on-plate welds have been employed to compare standard weld heat sources and develop the new heat source model. The proposed heat source is based on a combination of standard heat sources. From this study, it was concluded that the standard heat sources could not predict the observed melted zone for certain industrial application while the new heat source was able to do so. Therefore, the proposed heat source model can be employed to model keyhole mode laser welding, which enables welding simulation of a set of design proposals during the design process in a larger number of industrial cases.
New Correlations for Flow and Conjugate Heat Transfer With Surface Radiation Characteristics of a Real-Scale Infrared Suppression System With Conical Funnels
Authors: Mukherjee Arnab , Chandrakar Vikrant , Senapati Jnana Ranjan 
Published: May 2021

AbstractThe consistent and accurate prediction of fluid flow and heat transfer characteristics in an infrared suppression (IRS) device is challenging due to the complex nature of the flow features. The cool ambient air intake and subsequent mixing of hot exhaust gas from the engine in the cargo/naval ships are done inside the IRS system. The objective is to propose correlations for mass entrainment and outlet temperature for the IRS device with conical funnels. The mass intake rate and funnel exit temperature are determined by a set of relevant operating and geometric parameters, such as Reynolds number, nozzle exhaust temperature, the number of funnels, and funnel overlap. In this study, the funnel walls are conducting with finite wall thickness, and the surface radiation is taken into consideration. Numerical simulations are performed for the real-scale IRS unit by solving the mass, momentum, energy, and radiation equations in the computational domain surrounding the system. Nonlinear regression analysis of the data is carried out using the Levenberg and Marquest (L–M) method to achieve an empirical correlation of mass intake ratio and outlet temperature ratio. The proposed correlation for mass intake ratio is valid within ±6%, and that of outlet temperature is valid within ±5% of the numerical data. The valid ranges for correlations are 6×105≤ nozzle Reynolds number ≤3×106; 2 ≤ number of funnel ≤ 5; −0.325 ≤ funnel-overlapping height ≤ 0.25; and 1.33 ≤ nozzle exit temperature ≤ 2.
Microwave Versus Conventional Porcelain Firing: Greenware to Biscuit Crystallochemical Transformations
Authors: Santos T. , Gomes C. S. F. , Costa V. A. F. , Costa L. C. 
Published: May 2021

Abstract
This work reports and compares the structural crystallochemical transformations occurring during the microwave and conventional porcelain manufacture. Batches of greenware (just dried) porcelain pieces are microwave and electrically fired at increasing temperatures, from 420 °C up to 1100 °C. Crystallochemical transformations are identified by X-ray diffraction (XRD) analysis and compared the results from samples microwave and conventionally fired. Microwave fired samples show the full and rapid collapse of kaolinite structure for firing temperatures just above 500 °C, whereas the collapse of kaolinite structure of the electrically fired samples is progressive, from about 500 °C up to 950 °C. Muscovite structure totally collapses at about 950 °C for microwave fired greenware samples, whereas muscovite structure total collapse only occurs at about 1050 °C for electrically fired greenware samples. Microwave and electric firing lead to appreciable differences in the sanidine–orthoclase–microcline structural transformations. Mullite formation could be identified in the microwave fired samples at temperatures 50 °C lower than in the electrically fired ones, especially for the conventional firing temperatures above 1050 °C, the same temperature reported in the literature.
A General Expression for the Welding Tendon Force
Authors: Grams Mitchell R. , Mendez Patricio F. 
Published: May 2021

Abstract
This study presents a novel expression for the tendon force associated with residual stresses produced during welding of large, thin sections. A general engineering equation is presented as the combination of a closed-form expression, based on idealized treatment, and correction factors to account for the effects of temperature-dependent thermal and mechanical material properties. The closed-form expression corresponds to the assumption of constant material properties. A rigorous mathematical treatment is utilized to derive explicit, exact expressions for the temperature-dependent correction factors without the need for empirical correlations. The temperature-dependent behavior of materials is captured accurately using four dimensionless groups. The analysis was validated through numerical simulations with common structural grades of low-carbon steel, stainless steel, aluminum, and titanium. The idealized treatment resulted in predictions with a mean difference of 18%, which was reduced to 7% by incorporating the correction factors. The remaining error is a systematic overestimate, which can be attributed to compliance effects of the finite plate used in the simulations, and is the focus of ongoing research. The utility of applying the novel tendon force equation to problems in fabrication procedure design is demonstrated with an example predicting distortion during manufacturing of hollow structural sections.
Intuitive Physical Human–Robot Interaction Using an Underactuated Redundant Manipulator With Complete Spatial Rotational Capabilities
Authors: Audet Julien M. , Gosselin Clément 
Published: May 2021

Abstract
In this paper, the concept of underactuated redundancy is presented using a novel spatial two-degrees-of-freedom (2-DoF) gravity-balanced rotational manipulator, composed of movable counterweights. The proposed kinematic arrangement makes it possible to intuitively manipulate a payload undergoing 3-DoF spatial rotations by adding a third rotational axis oriented in the direction of gravity. The static equilibrium equations of the 2-DoF architecture are first described in order to provide the required configuration of the counterweights for a statically balanced mechanism. A method for calibrating the mechanism, which establishes the coefficients of the static equilibrium equations, is also presented. In order to both translate and rotate the payload during manipulation, the rotational manipulator is mounted on an existing translational manipulator. Experimental validations of both systems are presented to demonstrate the intuitive and responsive behavior of the manipulators during physical human–robot interactions.
The Hemolysis Effect of Rotary Cutting Thrombectomy Device: The CFD and Experimental Study
Authors: Gu Xuelian , Zhang Xiaoying , Qiu Xiaojian , Zheng Kang , Lu Licheng 
Published: May 2021

Abstract
Percutaneous mechanical thrombectomy (PMT) is an efficient way to treat the deep vein thrombosis (DVT). During the course of treatment, blood cell will be destroyed by the component of thrombectomy device, causing hemolysis. In this research, the computational fluid dynamics (CFD) methodology is used to investigate hemolysis effect of rotary cutting thrombectomy device. And a prototype of rotary cutting thrombectomy device is made to carry out two corresponding experiments to verify the simulation results. CFD results indicate that the hemolysis rate increases significantly with the rise of rotation speed of cutting component. The experimental results are in good consistence with the CFD results, and shows that the longer the working time, the higher the hemolysis rate. Thus a rotation speed between 5,000 rpm and 10,000 rpm can be considered reasonable. The results can be served as a helpful guidance for the design of such thrombectomy devices.
Design of Novel Baby Personal Protective Equipment: A Portable IOT-Based Baby Protective Tent
Authors: Liao Yu-Chi , Bu Hsing-Yun , Lee Pei-Jung , Lin Yu-Sheng , Weng Chen-Hsun 
Published: May 2021

Abstract
Due to the threat of COVID-19 pandemic, personal protection equipment (PPE) has raised lots of attentions to protect people from infection. This unprecedented virus can be spread through airborne, respiratory droplets and social contact. Although there are some devices and PPEs for people who are conscious and able to obey the social distance policy, to protect infants is still challenging. Baby is one of the vulnerable groups to the disease but there is no appropriate PPE when baby needs to be exposed to irresistible events like visiting a hospital, receiving vaccination or being accompanied by parents who come home from work). In this study, a concept of smart baby protective tent is developed. The simple hardware offers a quick assembly using PVC pipes, 3D-printed joints and a transparent cover sheet to create an independent and safe space from direct and indirect infection through breathing or saliva. With two additional parts, the smart baby protective tent can thereby provide clear air circulation and modern care. A fan system equipped with a clamp for a disposable medical-certified mask is applied to supply clean and circulated air for the babies. Functional sensors are attached to detect essential signals like body temperature, environment temperature, humidity, vibration, brightness, sound, CO2, and PM 2.5. These received signals are processed via a micro-controller and can be uploaded to personal social media in order to increase caregivers’ awareness of the babies’ situation inside the tent.
The Design and Use of a Minimally-Invasive, Expandable Retractor for Deep-Seated Brain Lesions
Authors: Yoo Sun Jay , Mou Jody , Elizebath Reena , Sivakumar Ananyaa , DeBrabander Rene , Shifman Mark , Tu Kevin , Ishida Wataru , Fouda Mohammed , Manbachi Amir , Cohen Alan R. 
Published: May 2021

Abstract
Access to deep-seated brain lesions (e.g., tumors, aneurysms, hematomas, and other malformations) is challenging due to the potential for retraction-induced injury. Traditionally, neurosurgeons use dissection and blade retractors to push apart tissue to visualize and operate on target lesions. These blades apply focal pressure onto the brain, resulting in ischemia, edema, and parenchymal trauma, leading to complications in up to 29% of cases. Tubular retractors were introduced to distribute forces radially and have led to improved safety and clinical outcomes. However, reports indicate that tubular retractors still led to complications in up to 9.1% of cases. Other concerns include significant pressure in the direction of insertion and the displacement of anatomic landmarks leading to inaccurate stereotaxis.
We present a novel, minimally-invasive brain retractor that utilizes an expandable soft balloon to further reduce retraction-induced injury and increase stereotactic accuracy with a minimal port of entry. The device consists of a balloon catheter system, a clear sheath, and integration with neuronavigation stylets. This approach can reduce the rate of iatrogenic injury and improve clinical outcomes for brain lesion operations. Furthermore, we illustrate the efficacy of this device in use compared to those of conventional tubular and blade retractors in a pig cadaver.
Imaging of Focused Ultrasound-Induced Shear Waves to Probe Mechanical Anisotropy of Tissue
Authors: Guertler Charlotte A , Okamoto Ruth J , Garbow Joel R , Chen Hong , Bayly Philip V 
Published: May 2021

Abstract
It is important to understand mechanical anisotropy in fibrous soft tissues because of the relationship of anisotropy to tissue function, and because anisotropy may change due to injury and disease. We have developed a method to noninvasively investigate anisotropy, based on MR imaging of harmonic ultrasound-induced motion (MR-HUM), using focused ultrasound (FUS) and magnetic resonance elastography (MRE). MR-HUM produces symmetric, radial waves inside a tissue, which enables a simple assessment of anisotropy using features of the resulting shear wave fields. This method was applied to characterize ex vivo muscle tissue, which is known to exhibit mechanical anisotropy. Finite element (FE) simulations of the experiment were performed to illustrate and validate the approach. Anisotropy was characterized by ratios of apparent shear moduli and strain components in different directions.
Automated Device for Public Elevator Control Panel UVC Sanitization
Authors: Hu Jia , O’Brien Jacob , Joshi Amey S. , Navabi Zahra S. , Alegria Andrew D. , Kodandaramaiah Suhasa B. 
Published: May 2021

Abstract
In response to the COVID-19 pandemic, studies have shown that frequently-touched surfaces that are contaminated with SARS-CoV-2 can pose a risk to public health and safety. Considering elevators as a high-risk environment for the spread of COVID-19 and other infectious diseases via surface transmission, common methods of manually applying liquid-form disinfectants are impractical for sanitizing the elevator panel after each use. Therefore, an automated UVC light surface sanitization device with integrated sensing components to avoid UVC light-human interaction and perform frequent sanitization was developed. Algorithmically, the system uses a motion sensor, an inertial measurement unit, and a door sensor to determine when the elevator is empty, stationary, and shut. Once these conditions are met, the UVC lamp is enabled to safely sanitize the elevator control panel. The device’s UVC irradiation capabilities were tested by applying UVC light to a mock control panel. A minimum power density of 0.31 mW/cm² was detected, which can deactivate SARS-CoV-2. The sensing and control system was tested in an elevator and it was demonstrated to be able to detect operating conditions and activate the UVC light at appropriate instances. Our device operates using inexpensive hardware and it can be easily integrated into existing elevator infrastructures.
Design of a Multi-Degree-of-Freedom Traction and Stabilization Device for Open Reduction and Internal Fixation of Distal Radius Fractures
Authors: Oeding J. , Bockman S. , Chiu H. , Hua C. , Connor J. , Slocum A. 
Published: May 2021

Abstract
Open reduction and internal fixation (ORIF) is a surgical procedure performed with the objectives of restoring normal alignment and providing stability to broken bone fragments after a fracture. This procedure is increasingly used to treat fractures of the distal end of the radius. Reduction of the fracture into correct alignment is often achieved by the surgeon pulling and manipulating the hand while looking at real-time x-rays, and frequently requires large forces to distract impacted fragments from proximal bone. This can make the task of simultaneously aligning the fracture fragments and placing hardware to secure fragments into the correct position particularly challenging for surgeons. This study presents the design and preliminary testing of a multi-degree-of-freedom (DOF) device capable of performing both distraction and reduction of fractured bone fragments using a traction splint mechanism with locking joints that results in significantly reduced effort and greater accuracy in performing ORIF procedures on distal radius fractures. Quantitative force testing found an 80% reduction in the maximum force required to create needed traction, while qualitative tests with a hand surgeon found the device’s ability to reduce and stabilize bone fragments while hardware is secured to be more intuitive and less obstructive than existing techniques.
3D Shape Estimation of an Active Needle Inside Tissue Using 2D Ultrasound Images
Authors: Konh Bardia , Batsaikhan Zolboo , Padasdao Blayton 
Published: May 2021

Abstract
This work presents a method to estimate 3D shape of an active needle inside tissue using 2D transverse ultrasound images. The shape of the needle provides a valuable feedback information for precise control and guidance of the needle inside tissue toward target. We used a series of image processing techniques to identify the needle’s cross section in the ultrasound images. Using this method, we estimated the 3D shape of a tendon-driven active needle, when bent inside a transparent phantom tissue using a robotic needle insertion system. The estimated shape of the needle was then compared with true shape of the needle captured by two cameras. At least three ultrasound images were required to estimate the needle shape with a second order polynomial function. We found an average error of 0.54mm and a maximum error of 1.00mm in shape estimation compared to the true shape of the needle captured by the cameras for an insertion depth of 70mm.
A Qualitative Comparison of the Covex Respirator with Face Shield Versus the N95 Respirator
Authors: Mimmack Reid , Germo Elijah , Augustine Garrett , Belani Kumar 
Published: May 2021

Abstract
In March 2020, the World Health Organization declared the novel coronavirus (COVID-19) outbreak a worldwide pandemic. The pandemic led to concerns of shortages regarding healthcare-related resources, including personal protective equipment (PPE), ventilators, and more. The uniquely designed COVEX respirator with face shield was engineered and manufactured by Augustine Surgical, Inc. to combat the PPE shortage. The novel COVEX mask is an “all-in-one” face shield and filter with a Viral Filtration Efficiency (VFE) greater than 99%. A standard respirator qualitative fit test was completed on the COVEX respirator as well as the current medical standard 3M N95 respirator. Fit test studies comparing the two masks yielded similar outcomes. The COVEX respirator had a qualitative fit test pass rate of 96.6% and the 3M N95 respirator had a pass rate of 93.3% (n=30). Participants also reported other variables comparing the comfort and fit of each mask, which is described further in the discussion. The COVEX respirator with face shield passed a standardized qualitative fit test at a rate similar to the current medical standard N95 respirator. Our results suggest that the COVEX mask may be a viable PPE option in the future.
Design of a Soft, Self-Uncoiling Stent for Extended Retention of Drug Delivery in the Small Intestine
Authors: Sarker Sunandita , Jones Ryan , Chow Gabriel , Terry Benjamin 
Published: May 2021

Abstract
Despite being the preferred route of drug administration, the oral formulation of biological drugs is limited due to its intrinsic instability, low permeability, and physical, chemical and immunological barriers. Various innovative swallowable technologies such as drug-loaded, dissolvable microneedles, mucoadhesive patches, and various microdevices present unique drug-carrying capabilities. The current work presents a novel soft stent platform that can facilitate contact between the small intestine tissue and drug carriers to enhance drug absorption and increase residence time. This study aims to prove the concept of this novel platform and determine if the soft stent will retain orally to the ileocecal valve longer than a capsule-shaped bolus. Benchtop studies on an intestinal simulator showed successful retention of the soft stent compared to a control capsule. In vivo studies in pig models also showed that the soft stent was retained longer than the control capsule. Overall, this study shows promise that this novel platform could be used for oral drug delivery of biologics.