bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Draft Genome Sequence of The Endophytic Streptomyces Strain VITGV156
Authors: P Veilumuthu , T Nagarajan , S Sasikumar , R Siva , Christopher J Godwin 
Published: August 2021

Abstract
Streptomyces species is one among the dominant group of bacteria in the family Actinobacteria with a rich repertoire of secondary metabolites. Secondary metabolites with antimicrobial activity and plant growth promotor have been isolated from various Streptomyces sp. Here in this investigation, we present the draft genome of a new species, Streptomyces sp. VITGV156 isolated from healthy tomato plant (Lycopersicon esculentum) which has some rare antimicrobial secondary metabolites, like coelichelin, fluostatins, vicenistatin, nystatin, sipanmycin, and informatipeptin. The genome is 8.18 Mb in size with 6,259 protein coding genes. The average GC content of the genome is 72.61 %. Preliminary analysis with antiSMASH 6.0 revealed the presence of 29 biosynthetic gene clusters for the synthesis of potential secondary metabolites. These includes 4 NRPS (non – ribosomal peptide synthetase), 7 PKS (Polyketide Synthases), 2 RiPP (Ribosomally synthesized and post-translationally modified peptides) clusters. When we look into genes associated with secondary metabolites, 406 genes are present which includes 184 genes for cofactor and vitamins, 72 genes for terpenoids and polyketides, 70 genes for xenobiotics and 80 genes for other metabolites are present. Comparative genome analysis of VITGV156 with its closest neighbor Streptomyces luteus strain TRM45540 revealed ANI 91.22% and dDDH value 44.00%.
Acute Lymphoblastic Leukemia Complicating Graves’ Disease in a Sudanese Adolescent Girl: A Case Report and Exploration of the Underlying Mechanism Possibilities
Authors: Fadlalbari Ghassan Faisal , Hassan Samar Sabir , Abdallah Asmahan T , Abusamra Samar Omer , Abdalrhman Abeer Mohamed , Abdullah Mohamed Ahmed 
Published: August 2021

Abstract
Background: Graves’ Disease (GD) related bone marrow injury presents usually as agranulocytosis or less commonly as pancytopenia. However, acute lymphoblastic leukemia (ALL) has been reported recently in an adult patient with GD. The underlying pathogenesis is not fully understood. Nevertheless, the harmful effect of anti-thyroid drugs or autoimmune reaction to bone marrow cells is anticipated to be the causative factors. Case report: A 16.5-year-old Sudanese girl with GD was on carbimazole for the first fourteen months of her illness, with irregular follow up, then it was withdrawn because she developed hypothyroidism for which she was put on thyroxine. Meanwhile, she developed severe anemia without fever which necessitated blood transfusion. Eight months later, she presented with thyrotoxicosis relapse, febrile illness and pancytopenia which was proved to be ALL on bone marrow examination. Conclusion: ALL must be considered when encountering a GD patient with pancytopenia.
Predation on Planted and Wild Bay Scallops (Argopecten Irradians Irradians) by Busyconine Whelks: Studies of Behavior Incorporating Acoustic Telemetry
Authors: Sclafani Matthew , Bopp Justin , Havelin Jason , Humphrey Corey , Hughes Scott WT , Eddings Justin , Tettelbach Stephen T. 
Published: August 2021

Abstract
Quantifying predator-prey interactions and gaining insights into predator behavior are crucial for optimizing restoration strategies; yet, such knowledge is often lacking for marine invertebrates. We examined potential impacts of predation by channeled (Busycotypus canaliculatus) and knobbed whelks (Busycon carica) on natural and planted populations of bay scallops in the Peconic Bays, New York, through laboratory and field investigations. Mean rates of predation by small channeled whelks were low: 0.06 and 0.005 scallops d-1 for adult and juvenile scallops; respective lab predation rates of small knobbed whelks on juvenile scallops were 24x higher. Channeled whelks usually did not damage shells of scallop prey (86%), but knobbed whelks typically notched the ventral shell margin (73%). In field plots where scallop densities were manipulated via removals/plantings, whelks consumed ~2% of ~19,100 planted juveniles; crabs and presumably finfish consumed over 40% in all trials overall. Acoustic telemetry revealed that tagged channeled whelks moved shorter distances and spent more time in plots planted with scallops versus those without scallops. Unexpectedly, whelks spent more time in low vs. high density plots, but consumed far more scallops in the latter. In trials without scallops, whelk movement rates were 5x higher, presumably due to increased exploratory behavior. Overall, whelks were most active during crepuscular hours and during periods of increasing wind speeds. Our results, combined with population abundance data, suggest that whelks (especially B. carica) are drawn to high density scallop plantings and may be important causes of bay scallop mortality in the context of restoration efforts.
Key Regulatory Pathways, microRNAs, and Target Genes Participate in Adventitious Root Formation of Acer Rubrum L.
Authors: Zhu Wenpeng , Zhang Manyu , Li Jianyi , Zhao Hewen , Zhang Kezhong , Ge Wei 
Published: August 2021

Abstract
BackgroundAcer rubrum L. is a colorful ornamental tree with great economic value. Because this tree is difficult to root under natural conditions and the seedling survival rate is low, vegetative propagation methods are often used. Because the formation of adventitious roots (ARs) is essential for the survival of asexual propagation of A. rubrum, it is necessary to investigate the molecular regulatory mechanisms in the formation of ARs of A. ruburm. To address this knowledge gap, we sequenced the transcriptome and sRNA of the A. rubrum variety ‘Autumn Fantasy’ using high-throughput sequencing and explored changes in gene and microRNA (miRNA) expression in response to exogenous auxin treatment. ResultsWe identified 82,468 differentially expressed genes between the treated and untreated ARs, as well as 48 known and 95 novel miRNAs. We also identified 172 target genes of the known miRNAs using degradome sequencing. Two regulatory pathways (ubiquitin mediated proteolysis and plant hormone signal transduction), Ar-miR160a and the target gene ArARF10 were shown to be involved in the auxin response. We further investigated the expression patterns and regulatory roles of ArARF10 through subcellular localization, transcriptional activation, plant transformation, qRT-PCR analysis, and GUS staining. ConclusionsDifferential expression patterns indicated the Ar-miR160a-ArARF10 interaction might play a significant role in the regulation of AR formation in A. rubrum. Our study provided new insights into mechanisms underlying the regulation of AR formation in A. rubrum.
Harnessing Optoelectronic Noises in a Hybrid Photonic Generative Adversarial Network (GAN)
Authors: Wu Changming , Yang Xiaoxuan , Yu Heshan , Peng Ruoming , Takeuchi Ichiro , Chen Yiran , Li Mo 
Published: August 2021

Abstract
Integrated programmable optoelectronics is emerging as a promising platform of neural network accelerator, which affords efficient in-memory computing and high bandwidth interconnectivity. The analog nature of optical computing and the inherent optoelectronic noises, however, make the systems error-prone in practical implementations such as classification by discriminative neural networks. It is thus imperative to devise strategies to mitigate and, if possible, harness optical and electrical noises in optoelectronic computing systems. Here, we demonstrate a prototypical hybrid photonic generative adversarial network (GAN) that generates handwritten numbers using an optoelectronic core consisting of an array of programable phase-change optical memory cells. We harness optoelectronic noises in the hybrid photonic GAN by realizing an optoelectronic random number generator derived from the amplified spontaneous emission noise, applying noise-aware training by injecting additional noise to the network, and implementing the trained network with resilience to hardware non-idealities. Surprisingly, the hybrid photonic GAN with hardware noises and inaccuracies can generate images of even higher quality than the noiseless software baseline. Our results suggest the resilience and potential of more complex hybrid generative networks based on large-scale, non-ideal optoelectronic hardware. The demonstrated GAN architecture and the proposed noise-aware training approach are generic and thus applicable to various types of optoelectronic neuromorphic computing hardware.
Health Risk behaviours Among Adolescents in Argentina: Trends Between 2007, 2012 and 2018 National Cross-Sectional School Surveys
Authors: Peltzer Karl , Pengpid Supa 
Published: August 2021

Abstract
Background

The aim of this study was to assess trends of various health risk behaviours among adolescents across three different surveys in Argentina.
Methods

Data from 115,697 adolescents (mean age:14.6 years, SD = 1.2) that participated in three cross-sectional national school surveys in 2007, 2012 and 2018 were analysed. In all, 27 health risk behaviours were assessed through a self-administered questionnaire. Significance of a linear trend was tested by treating study year as categorical variable in logistic regression analyses, adjusted by age group and food insecurity for boys and girls separately
Results

Among both sexes, four health risk behaviours (current cigarette use, passive smoking, trouble from alcohol use, and physically attacked) significantly reduced from 2007 to 2018. Among boys five health risk behaviours (experience of hunger, parental tobacco use, current alcohol use, involvement in physical fighting, and multiple sexual partners), and among girls, inadequate physical activity significantly reduced over time. Among both sexes, the prevalence of four health risk behaviours (overweight/obesity, obesity, leisure-time sedentary behaviour and insufficient fruit intake) significantly increased among both sexes, and among girls ten health risk behaviours (not walking/biking to school, current other tobacco use, bullying victimisation, lifetime drunkenness, having no close friends, suicide plan, suicidal ideation, worry-induced sleep disturbance, loneliness, and ever sexual intercourse) significantly increased over time.
Conclusion

Nine health risk behaviours among boys and five health risk behaviours among girls decreased, and four health risk behaviours among boys and 14 health compromising behaviours among girls increased over a period of 11 years. School health programmes for adolescents should be strengthened in Argentina.
Genetic Determinants of Biofilm Formation and Antibiotic Resistance of Helicobacter Pylori using Whole Genome Sequencing
Authors: Fauzia Kartika Afrida , Aftab Hafeza , Miftahussurur Muhammad , Waskito Langgeng Agung , Tuan Vo Phuoc , Matsumoto Takashi , Yurugi Michiyuki , Subsomwong Phawinee , Tshibangu-Kabamba Evariste , Akada Junko , Yamaoka Yoshio 
Published: August 2021

Abstract
The nucleotide polymorphisms (SNPs) associated with the biofilm formation phenotype of Helicobacter pylori were investigated. Fifty-six H. pylori isolates from Bangladeshi patients were included in this cross-sectional study. Crystal violet was used to classify the phenotypes into high- and low-biofilm formers. Whole genome sequences were analyzed using the “Antimicrobial Resistance Identification By Assembly” (ARIBA) pipeline. The results indicated 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers. Mutations associated with antibiotic resistance can be detected. This study revealed the potential role of SNPs to biofilm formation, and propose a method to detect mutation in antibiotic resistance and biofilm from whole genome sequences.
Triggering Mitochondrial Dysfunction and Apoptosis Under Chronic Low-dose Lambda-cyhalothrin Exposure Modulated by Melissa Officinalis L Methanol Extract in Rat Hippocampus
Authors: Benaicha Brahim , Rouabhi Rachid , GASMI Salim , Menaceur Fouad , Mennai Imad , Lakroun Zhoura , Fetoui Hamadi , Kebieche Mohamed , Soulimani Rachid 
Published: August 2021

Abstract
The extensive use of Lambda-cyhalothrin (LCT) has been associated with the various toxicities that non-target organisms can undergo including mammals. However, the mechanism of induced-LCT cytotoxicity in animal brain cells is still elusive, particularly in brain regions, notably the hippocampus, an area directly involved in cognitive function. This study aimed to investigate the neurotoxic effects in the rat hippocampus chronically exposed to LCT (0.18 mg/kg and 0.36 mg/kg), and the neuroprotective potential of Melissa officinalis L methanol extract (MOE) (200 mg/kg) against this toxicity. After experimental period (90 days), the redox status, the functional and structural integrity of the hippocampus mitochondria as well as the apoptotic signaling pathway were evaluated. The current findings suggest that LCT produces imbalance of mitochondrial redox status expressed by, on one side, increasing of stress markers such as protein carbonyls (PCO), malondialdehyde (MDA), and hydrogen peroxide (H2O2) levels; and on the other side, by a decline in the potential of antioxidant systems, namely the level of mitochondrial enzymatic activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione (GSH).This study also showed an increase in mitochondrial permeability, along with mitochondrial edema and considerable decrease in its O2 consumption. Moreover, the same results recorded an increase in caspase-3 and cytosolic cytochrome-c. Conversely, this study proved that all these toxic aspects induced by LCT were significantly mitigated when the administration of this synthetic pyrethroid was associated with MOE. Taken together, data of this study shed light on mitochondrial damage and apoptosis stimulation under the toxic effect of LCT and suggests that MOE is endowed with potent neuroprotective effect, possibly via its antioxidant and antiapoptotic properties.
Incidence of COVID-19 Infection in Hospital Workers From March 1, 2020 to May 31, 2021 Routinely Tested, Before and After Vaccination With BNT162B2
Authors: Filon Francesca Larese , Rui Francesca , Ronchese Federico , Michieli Paola , Negro Corrado 
Published: August 2021

Abstract
Objective

To evaluate the incidence of COVID-19 infection in health care workers from the start of COVID-19 pandemic in NE of Italy, to the vaccination with BNT162b2.
Materials and methods

This was a retrospective cohort study. Health care workers were routinely tested for SARS-CoV-2 infections using real-time polymerase chain reaction tests in nasopharyngeal swabs. Logistic regression was used to calculate incident rate ratios (IRRs) of factors associated to COVID-19.
Results

A total of 4251 workers were followed-up and an annual incidence of COVID-19 of 13.6% was found. In March 2021 the incidence of infection was 4.88 and 103.55 cases for 100.000 person-days in vaccinated and non-vaccinated workers, respectively, with an adjusted IRRs of 0.05 (95% CI 0.02–0.08).
Conclusions

Our study evaluated the monthly incidence in health care workers in Trieste hospitals before and after the vaccination finding the protective effect of BNT162B2 vaccine in 95% of health care workers routinely tested.
Characterization of New Inorganic- Organic Hybrid Compounds: (C6H10N2).Cl2 (I), [C6H10N2]2ZnCl4 (II): Crystal Structure, Hirschfield Surface, IR and NMR spectroscopy Analysis.
Authors: hannachi nejeh , hlel faouzi 
Published: August 2021

Abstract
Two new organic-inorganic hybrid materials, (C6H10N2).Cl2 (I) and [C6H10N2]2ZnCl4 (II), have been synthesized by hydrothermal method and characterized by single-crystal X-ray diffraction and XRD pattern investigations. These two compounds are crystallized in the monoclinic system; C2/c space group. In the both structures, the anionic-cationic entities are interconnected by hydrogen bonding contacts and p-p Interaction forming three-dimensional networks. Intermolecular interactions were investigated by Hirshfeld surfaces and the contacts of the four different chloride atoms in (II) were compared. The vibrational absorption bands were identified by infrared spectroscopy. These compounds were also investigated by solid state 13C NMR spectroscopy.
Environment-friendly High Efficiency CH3NH3PbI3 Perovskite Solar Cells Fabricated by Green Antisolvent Method
Authors: gao bowen , MENG JING 
Published: August 2021

Abstract
Green antisolvents propyl acetate and isooctane can promote the nucleation of PbI2 particles and provide the heterogeneous nucleation site of perovskite crystal, so as to promote the rapid growth of perovskite crystal. The films treated with propyl acetate and propylene glycol methyl ether have larger grains and lower root-mean-square value (RMS) than those treated with chlorobenzene. The full coverage perovskite films with uniform grain size which is closer to the exciton diffusion length of perovskite can be obtained. Compared with the CH3NH3PbI3 perovskite solar cells treated with chlorobenzene (power conversion efficiency (PCE) is 17.86%), the best efficiency of the devices treated with propylene glycol methyl ether is 21.60%, which is improved by 21%, which has certain reference value and guiding significance for researchers to obtain environment-friendly high-quality perovskite solar cells in the future.
CircZNF236 Facilitates Malignant Progression in Oral Squamous Cell Carcinoma by Sequestering miR-145-5p
Authors: Lu Qing , Che Yanhai , Che Hongze , Hu Min 
Published: August 2021

Abstract
BackgroundA number of non-coding circular RNAs (circRNAs) have recently been implicated in the modulation of gene expression in cancer models. We therefore sought to explore if circZNF236 has a role in oral squamous cell carcinoma (OSCC).MethodsWe first examined circZNF236 expression in 32 pairs of OSCC and noncancerous tissues. We then investigated a functional role for circZNF236 using knockdown and overexpression approaches in OSCC cancer cell lines. Cell counting kit-8, wound healing, Transwell, and flow cytometry were employed to assess circZNF236 function in vitro. The association between circZNF236 and miR-145-5p, or that between miR-145-5p and malignant brain tumour domain containing 1 (MBTD1) was predicted by bioinformatics and demonstrated by dual-luciferase reporter assays, RNA pull-down assays as well as RNA immunoprecipitation (RIP) assays. A mouse OSCC xenograft model was employed to demonstrate the impacts of circZNF236 inhibition on tumor development in vivo. ResultsOSCC tissues and cells had higher levels of circZNF236 expression compared with normal controls. Furthermore, high circZNF2361 levels in patients with OSCC correlated with a poor prognosis. CircZNF236 silencing decreased the malignant properties of OSCC cells and suppressed OSCC tumor formation in the mouse model. We then noticed that miR-145-5p can be regulated by circZNF236, and that circZNF2361 promoted OSCC development by absorbing miR-145-5p and consequently upregulating MBTD1 expression.ConclusionCircZNF236 modulates OSCC via an miR-145-5p/MBTD1 axis. These results support the potential of circZNF236 as a treatment target for OSCC.
High-intensity Physical Activity Is Not Associated With Better Cognition in the Elder: Evidence From the China Health and Retirement Longitudinal Study
Authors: Wu Zhiyuan , Zhang Haiping , Miao Xinlei , Li Haibin , Pan Huiying , Zhou Di , Liu Yue , Li Zhiwei , Wang Jinqi , Liu Xiangtong , Zheng Deqiang , Li Xia , Wang Wei , Guo Xiuhua , Tao Lixin 
Published: August 2021

Abstract
Objective To evaluate the association of physical activity (PA) intensity with cognitive performance at baseline and during follow up. Methods A total of 4 039 participants aged 45 years or above from the China Health and Retirement Longitudinal Study were enrolled in visit 1 (2011-2012), and followed for cognitive function in visit 2 (2013-2014), visit 3 (2015-2016) and visit 4 (2017-2018). We analyzed the association of PA intensity with global cognition, episodic memory and mental intactness at baseline using adjusted regression methods, and evaluated the long-term effect of PA intensity using multiple measures of cognition scores by mixed effect model.Results Compared with individuals without PA, those with mild PA had 0.42 (3.7%) higher global cognition, and 0.20 (5.9%) higher episodic memory, while individuals with moderate PA had 0.59 (5.3%) higher global cognition, 0.25 (7.1%) higher episodic memory, and 0.34 (4.4%) higher mental intactness at baseline. The vigorous PA was not associated with better global cognition, episodic memory and mental intactness (all P>0.05). The results remained almost consistent in multiple sensitivity analyses. In addition, mild and moderate PA, rather than vigorous PA, was beneficial to long-term cognitive performance. Conclusions Our study indicates that mild and moderate PA could improve cognitive performance, rather than the vigorous activity. The targeted intensity of PA might be more effective to achieve the greatest cognition improvement considering age, overweight and depressive status.
Mapping Publication Trends and Knowledge Structure on Non-Invasive Prenatal Testing – A Bibliometric Analysis
Authors: Fan Ting , Zhang Bijun 
Published: August 2021

Abstract
Background: Non-invasive prenatal testing (NIPT) has become the widely adopted prenatal testing for screening the common aneuploidy of pregnant woman and even expented to detecting copy number variations (CNVs) and Monogenic disease. In recent 10 years, NIPT has become an increasingly important scholarly focus. However, date on long-term bibliometric studies on NIPT has not been reported. Objective: The purpose of this study was to map publications trends and to explore the knowledge structure on NIPT. Methods: Publications were collected from database PubMed in the past 10 years. A bibliometric analysis based on PubMed was conducted to investigate the publication trends and the knowledge structure. Co-word blustering analysis and strategic diagram of highly frequent major MeSH terms were utilized to detect the hot spots in this field. Results: A total of 2160 articles about NIPT were retrieved according to our search strategy in this study. Article was the main document type. The 10 most active journals published 29.3% (633/2160) of the publications in this area. By analyzing and summarizing the highly frequent major MeSH, 7 research hot spots were founded.Conclusion: Our data indicated that NIPT mainly focuses on the 7 major research hot spots, and strategic diagram provides some hints for the future research trend of NIPT.