bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adrenal Crisis and Disseminated Intravascular Coagulation Associated With Disseminated Carcinomatosis of the Bone Marrow From Gastric Cancer
Authors: Lee Roland Y , Goldberg Elliott , Malysz Jozef , Li Huili , Shin Benjamin , Chen Xueying 
Published: March 2021

Abstract
Gastric cancer is the third leading cause of cancer deaths worldwide. Disseminated carcinomatosis of bone marrow (DCBM) originating from gastric cancer is rare and associated with poor prognosis. We present the first reported case of gastric adenocarcinoma with DCBM leading to concomitant disseminated intravascular coagulation (DIC) and adrenal crisis refractory to treatment. Computerized tomography of the chest, abdomen and pelvis showed diffuse sclerosis of the axial and proximal appendicular skeleton, suggestive of metastatic disease. Bone marrow biopsy demonstrated infiltrative signet ring cells, consistent with metastatic adenocarcinoma from gastric primary. Laboratory tests showed severe anemia, thrombocytopenia, and coagulopathy, compatible with DIC.
Single Nucleotide Polymorphism Analysis of Pvmdr-1 in Plasmodium Vivax Isolated From Military Personnel of Republic of Korea in 2016 and 2017
Authors: Bong Jin-Jong , Lee Wonsig , Park Qu-Ehn , Noh Kyung Tae 
Published: March 2021

Abstract
Background: Malaria chemoprophylaxis using chloroquine and primaquine has been administered to resident soldiers in the 3rd Army of Republic of Korea (ROK) to prevent malaria infection since the year 1997. Due to mass chemoprophylaxis against malaria, concern exists about occurrence of chloroquine resistance. Herein, we investigated the single nucleotide polymorphisms (SNPs) of the Plasmodium vivax multi-drug resistance protein-1 (Pvmdr-1) gene to monitor the risk of chloroquine resistance.Methods: To evaluate the risk of malaria chemoprophylaxis, SNPs of the Pvmdr-1 gene were analysed in 73 soldiers of the 3rd Army of ROK diagnosed with infection by Plasmodium vivax. Results: Quintuple mutations (G698S, L845F, M908L, T958M, and F1076L) were detected in 73 soldiers. Mutation in the Y541C position was detected in soldiers at a frequency of 1.3% (1/73). In addition, silent mutations were detected at positions 44K, 493L, 529T, and 1233E. Based on these SNPs, Pvmdr-1 sequences of ROK were classified into 6 haplotypes. Phylogenetic analysis showed that the neighbourhood of the 6 haplotypes were Chaina_NB-16 and Papua New Guinea-PNG58 (Figure 1).Conclusions: Genetic- or phenotypic-based chloroquine resistance was not observed. However, various SNPs including a newly identified non-synonymous mutation (Y541C) have been introduced into Plasmodium vivax malaria endemic areas in ROK. Thus, to prevent the emergence of chloroquine resistance, continuous surveillance for SNPs of Pvmdr-1 related with chloroquine resistance is essential in the malaria chemoprophylaxis-executed regions of ROK.
Glial Cells Modulate Retinal Cell Survival in Rotenone-Induced Neural Degeneration
Authors: Tawarayama Hiroshi , Inoue-Yanagimachi Maki , Himori Noriko , Nakazawa Toru 
Published: March 2021

Abstract
Administration of the mitochondrial complex I inhibitor rotenone provides an excellent model to study the pathomechanism of oxidative stress-related neural degeneration diseases. In this study, we examined the glial roles in retinal cell survival and degeneration under the rotenone-induced oxidative stress condition. Mouse-derived Müller, microglial (BV-2), and dissociated retinal cells were used for in vitro experiments. Gene expression levels and cell viability were determined using quantitative reverse transcription-polymerase chain reaction and the alamarBlue assay, respectively. Conditioned media were prepared by stimulating glial cells with rotenone. Retinal ganglion cells (RGCs) and inner nuclear layer (INL) were visualized on rat retinal sections by immunohistochemistry and eosin/hematoxylin, respectively. Rotenone dose-dependently induced glial cell death. Treatment with rotenone or rotenone-stimulated glial cell-conditioned media altered gene expression of growth factors and inflammatory cytokines in glial cells. The viability of dissociated retinal cells significantly increased upon culturing in media conditioned with rotenone-stimulated or Müller cell-conditioned media-stimulated BV-2 cells. Furthermore, intravitreal neurotrophin-5 administration prevented the rotenone-induced reduction of RGC number and INL thickness in rats. Thus, glial cells exerted both positive and negative effects on retinal cell survival in rotenone-induced neural degeneration via altered expression of growth factors, especially upregulation of microglia-derived Ntf5, and proinflammatory cytokines.
Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody
Authors: Osawa Yoshihiro , Nomura Kaho , Kimira Yoshifumi , Kushibe Seiji , Takeyama Ken-ichi , Nagao Masashi , Kataoka-Matsushita Aya , Koizumi Seiko , Mano Hiroshi 
Published: March 2021

Abstract
Active collagen oligopeptides are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC-MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.
Protective Effect of SCUBE1&nbsp;on Oxidative Stress-induced Apoptosis in Human Ovarian Granulosa Cells
Authors: Fu Huijiao , Cai Xuzi , Liu Qiwen , Yang Wei , wang xuefeng 
Published: March 2021

Abstract
Background: Apoptosis of ovarian granulosa cells (GCs) is a sign of follicular atresia. This study aimed to explore the role and mechanism of signal peptide, CUB domain, epidermal growth factor-like protein1 (SCUBE1) in protecting GCs from apoptosis induced by hydrogen peroxide (H2O2). Methods: Firstly, the expression of SCUBE1 on the ovaries of humans and mice was analyzed by qRT-PCR, western blot and immunohistochemistry. Subsequently, the H2O2 treated GCs were pretreated with SCUBE1 recombinant protein, and their cell viability and proliferation were detected by Cell Counting Kit-8 (CCK-8) assay. The levels of reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) in the cells were determined by DCFH-DA and rhodamine 123, respectively. The percentage of apoptotic cells was analyzed by flow cytometry after staining with Annexin V/PI. The expression levels of pathway related proteins, such as Bcl-2, Bax, p53, caspase-3, were determined by western blot analysis. Finally, the pathogenicity of SCUBE1 (c.1169C>G, p.P390R) were analyzed based on the software.Results: SCUBE1 was expressed in women of all ages and had the highest expression level in the ovaries in multiple organs and tissues of KM mouse. In vitro cell experiments show that SCUBE1 pretreatment reduced H2O2-induced apoptosis and improved cell viability. SCUBE1 also blocked the production of ROS in cells and improved mitochondrial membrane potential. After SCUBE1 pretreatment, anti-apoptotic protein Bcl-2 expression was upregulated, whereas the expression of the pro-apoptotic proteins Bax, Bax/Bcl-2, Caspase-3, and p53 were downregulated. Analysis of the impact of SCUBE1 (c.1169C >G, p.P390R) mutation from the aspect of mutation pathogenicity; protein stability; and gene haplotype insufficiency, indicated that the p.P390R mutation is significantly pathogenic.Conclusions: This is the first time that the potential role of SCUBE1 in protecting GCs from H2O2-induced damage through the mitochondrial pathways, attributing to POI, is studied. SCUBE1 (c.1169C >G, p.P390R) mutation has significant pathogenicity but the specific harm needs to be confirmed by further studies. Trial registration: Not applicable.
A Comparative evaluation of serum CRP levels in chronic and aggressive periodontitis patients before and after non-surgical periodontal therapy- a clinical study
Authors: Gupta Shruti , Gupta Ira , Gupta Rohit , Amaranath  B J Janardhana , Goswami Arpita , Singh Deepshikha 
Published: March 2021

Aims and Objectives: C-reactive protein is an acute-phase-reactant primarily produced by the liver in response to infection or trauma. Recent studies have demonstrated a correlation between periodontitis and elevated CRP levels. This study aims to relate the serum-CRP level in chronic and aggressive periodontitis patients, before and after periodontal treatment, with healthy controls.
Materials and Method: A case-control clinical study was conducted with a total of 75 systemically healthy subjects, where 25 subjects were selected in each groups: Group I, Healthy control subjects; Group II, generalized chronic periodontitis patients, and Group III, generalized aggressive periodontitis patients. Serum-CRP levels were quantified by using turbidimetric immunoassay at baseline and 3month post-treatment. Kit used was “TURBILYTE-CRP” (Tulip Diagnostics, Goa, India). In the treatment phase, patients received single visit nonsurgical periodontal treatment, completed within 24hrs under LA for GCP & GAP group.
Results: Mean serum CRP levels were significantly higher in both GCP and GAP groups as compared to the control group at baseline. On comparing the clinical parameters at 3 months post-treatment for GCP & GAP group with control group values, the mean score of serum CRP levels for the GAP group was statistically significant (P<0.001) in comparison to the GCP group, which in turn was statistically significant as compared to control group.
Conclusion: The present study indicates a positive correlation between CRP and periodontal disease severity with particular concern in younger individuals, where it could be a possible underlying pathway in the association between periodontal disease and risk for cardiovascular disease in periodontitis patients.
Risk factors for increased intraocular pressure and eye damage during robotic-assisted prostatectomy
Authors: Lutfarakhmanov I. I. , Galeev I. R. , Lifanova A. D. , Mironov Р. I. 
Published: March 2021

Prostate cancer remains the most common urologic malignancy, and robotic-assisted radical prostatectomy makes the most effective treatment option. The special conditions of the surgery (Trendelenburg position) affect the intraocular pressure.The objective: to systematize new data on changes in intraocular pressure during robotic-assisted radical prostatectomy under various types of general anesthesia. Materials and methods. Publications were reviewed using the PubMed search engine in the electronic databases of Medline, Embase, and Cochrane Library up to August 2020. Results. A total, 9 studies were included in the review including a randomized controlled one. Robot-assisted radical prostatectomy can be a safe surgery regarding perioperative changes in intraocular pressure and ophthalmic complications.Conclusions. The review offers the first assessment of changes in intraocular pressure during robotic-assisted radical prostatectomy. Further studies with a longer follow-up period are needed to determine the clinical efficacy and safety of various types of general anesthesia.
Enoxaparin-induced hepatotoxicity: clinical cases and literature review
Authors: Krovko Yu. N. , Shmigelskiy A. V. , Аkhmedov A. D. , Shulgina A. A. , Lukshin V. A. , Usachev D. Yu. 
Published: March 2021

Low molecular weight heparins, in particular enoxaparin, have a wide range of applications, including prevention and treatment of deep vein thrombosis. The most common adverse effects of these drugs are bleeding and thrombocytopenia, but a clinician should be aware of another less common but not less important adverse effect such as an elevated transaminase level. In 2019, we observed two cases of enoxaparin-induced hepatotoxicity. In the first one, enoxaparin 0.4 ml subcutaneously twice a day was prescribed to a 40-year-old woman as a bridge therapy to discontinue warfarin before elective surgery. In the second case, a 27-year-old man received enoxaparin 0.4 ml subcutaneously twice a day for the prevention of deep vein thrombosis. The elevation of transaminase level for more than 3 times above the norm was noted in both patients which required discontinuation of the drug.
Pengaruh Pengetahuan Keselamatan dan Kesehatan Kerja (K3) Terhadap Perilaku Pekerja dan Kecelakaan Kerja Pada Proyek di DS LNG Kabupaten Banggai Propinsi Sulawesi Tengah
Authors: Suartana P. , Mandagi R.J.M. , Wilar D. 
Published: March 2021

In association with the potential risks of work accidents in the implementation of construction work, the knowledge of K3 on a construction project is now a fundamental requirement. Aspects of K3 itself will not be able to be run as it should without any intervention from management in the form of a planned effort to manage it (safety management), which is often called the Occupational Safety and Health Management System (SMK3). This research aims to know effects of the knowledge of K3 on the behavior of construction workers as viewed from several aspects related to K3 such as the influence of knowledge variables K3 together to the behavior of construction workers, the effect of knowledge variables K3 partially on the behavior of construction workers and the effect of worker's behavior on work accident.This research were conducted in four construction companies which executed works given by the local government of Luwuk Banggai Regency in 2016. The research approach used in this research is survey method and regression and correlation analysis method having samples of 129 workers. Based on statistical test of hypothesis test, the test result of each K3 knowledge variable shows that all the variables tested (5 K3 knowledge variables ie devinition and initiation K3/X1, K3/X2 management system, personal protector K3/X3, facilities and infrastructure K3/X4, K3/X5 risk) has a strong correlation to worker behavioral variables (Y1). This is evidenced by the correlation numbers R> 0.8 and the determination (r2)>0.7. However, based on the results of simultaneous test (F test) to know the correlation of the five variables of K3 knowledge together to the variable of worker behavior (Y1), the result of statistical test on the simultaneous test shows that in t test (partial) only 2 K3 Good correlation to worker behavior (Y1) that is variable of personal protective device (X3) and K3 risk (X5). According to these two test, it revealed that linear multiple regression result in an equation: Y=0,318X3+0,557X5+1,613 with determinant coefficient (R2) = 71.4 % which means that the model resulting having very good performance.
A Rare Complications of Sickle Cell Disease: Empyema and Chronic Subdural Hematoma
Authors: Miraulle Tien Yu Song , Synèse Bemora Joseph , D’assise Masina Ndalana , Charles Rasolonjatovo Emilson , Clément Andriamamonjy , Rabarijaona Mamiarisoa , Willy Ratovondrainy 
Published: March 2021

Sickle cell disease is a hemoglobinopathy responsible for many complications that are sometimes frightening. Brain damage is part of one of the complications that can affect the vital and/or functional prognosis of patients. We report two cases of neurological complications, patients with known homozygous sickle cell disease, admitted to the Resuscitation Service of the JRA University Hospital for neurological deficit of recent appearance. One presented a spontaneous bilateral chronic bilateral subdural hematoma, and the other an extradural empyema diagnosed on brain scan. The patients benefited from a neurosurgical intervention with favorable postoperative outcomes, marked by an improvement in neurological symptoms. This form of complication is relatively rare in patients with sickle cell disease. It is favoured by repeated vaso-occlusive crises responsible for vascular fragility following repeated ischemia and inflammatory phenomena. The surgical indication depends on the clinical condition and the radiological aspect
Identifikasi Spesies Streptokokus ?-Hemolisis Hasil Isolasi dari Nasal dan Tonsil Babi dengan Uji Basitrasin
Authors: Suardana I Wayan , Dinarini Ni Made Ayu Aryati , Sukrama I Dewa Made 
Published: March 2021

Streptokokosis merupakan penyakit yang disebabkan oleh kelompok bakteri Streptococcus sp dengan manifestasi berupa poliartritis, septicemia, dan meningitis. Strain Streptokokus ?-hemolisis merupakan salah satu bakteri patogen yang dapat masuk melalui luka, abrasi, makanan atau saat sistem imun melemah. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri Streptokokus ?-hemolisis pada babi yang diternakkan di peternakan rakyat Desa Punggul dan Desa Bongkasa, Kecamatan Abiansemal, Badung, Bali. Studi ini menggunakan 47 isolat berasal dari swab mukosa hidung dan tonsil. Semua sampel dikultur pada media agar darah dilanjutkan dengan penanaman pada media Brain Heart Infussion (BHI), kemudian dilakukan pewarnaan Gram, uji katalase dan uji biokimiawi. Setelah didapati isolat positif Streptokokus ?-hemolisis, dilanjutkan dengan pengujian basitrasin untuk mendeteksi strain Streptococcus pyogenes dan strain beta hemolisis lainnya. Hasil penelitian menunjukkan dari 47 isolat yang diuji, sebanyak 8 isolat (17%) merupakan bakteri presumtif Streptokokus ?-hemolisis. Hasil uji basitrasin terhadap ke-8 isolat tersebut ditemukan 3 isolat (37,5%) menunjukan zona hambat basitrasin dan dikelompokkan sebagai S. pyogenes, sedangkan 5 isolat (62,5%) lainnya tidak menunjukan zona hambat dan dikelompokan sebagai Streptokokus ?-hemolisis non-pyogenes.
Karakteristik Personal dalam Memoderasi Pengaruh Penganggaran Partisipatif dan Ambiguitas Peran pada Senjangan Anggaran
Authors: Dharmawan Yoana , Wirakusuma Made Gede 
Published: March 2021

This study aims to determine the role of personal characteristics in moderating the effect of participatory budgeting on budgetary slack and to determine the role of personal characteristics in moderating the influence of role ambiguity on budgetary slack. Data used is primer data, the analysis technique used in this study is Moderated Regression Analysis. The results of the analysis address personal characteristics which weaken the effect of participatory budgeting on budgetary slack. This shows that with good personal characteristics, participatory budgets will be able to reduce budgetary slack events. Personal characteristics weaken the influence of role ambiguity on budgetary slack. This shows that good personal characteristics, the role ambiguity will be reduced and will able to reduce the budgetary slack.
Keywords: Personal Characteristics; Participatory Budgeting; Role Ambiguity; Budgetary Slack.
Pengaruh Pendapatan Asli Daerah, Belanja Modal dan Indeks Pembangunan Manusia
Authors: Hobrouw Yustine Asnad , Suaryana I Gusti Ngurah Agung , Asri Dwija Putri I G.A.M. 
Published: March 2021

This study aims to determine empirically the effect of local revenue on the increase in the human development index in West Papua province and to empirically determine the effect of capital expenditure on the increase in the human development index in West Papua province. The data analysis technique used is multiple linear regression analysis. Based on the results of the analysis, it is known that the Original Regional Income has a positive and significant effect on the increase in the District / City Human Development Index in West Papua Province. This shows that an increase in Regional Original Income (PAD) will tend to increase the Human Development Index. Capital expenditure has a positive and significant effect on increasing the District / City Human Development Index in West Papua Province.
Keywords: PAD; Capital Expenditures; Human Development Index.
ЯКІСТЬ ЖИТТЯ ЖІНОК ПІСЛЯ НЕХІРУРГІЧНОЇ КОРЕКЦІЇ ПОЧАТКОВИХ ПРОЯВІВ ГЕНІТАЛЬНИХ ПРОЛАПСІВ
Authors: Yu. V. Lavrenuik  , K. V. Chaika  , L. V. Suslikova  , A. V. Serbenuik  , O. P. Barishnikova  
Published: March 2021

Мета роботи - оцінити вплив симптомів початкових проявів генітальних пролапсів (ГП) на якість життя жінок.
Матеріал та методи дослідження. У дослідження було включено 120 пацієнток з ГП I – II стадії, які склали дві групи: основну – 90 пацієнток, розподіленні на три підгрупи: Ia (n = 30) – корекція ГП, проведена із застосуванням аутоплазми, збагаченої тромбоцитами, Iб (n = 30) - лікування пролапсу препаратом на основі гіалуронової кислоти (Neauvia Organic Intense Rose, Швейцарія), Iв (n = 30) – використання лазерної системи CO2 (SmartXideDOT, DEKA, Італія). Групу порівняння склало 30 пацієнток корекція ГП у яких здійснювалась шляхом тренування м’язів тазового дна в індивідуальному режимі згідно з стандартною методикою.
Результати дослідження. Отриманні результати при вивченні якості життя жінок, яким проведено нехірургічну корекцію початкових стадій ГП, дозволяє виокремити, що показник обмеження повсякденної фізичної активності у пацієнток з ГП до корекції мав найгіршу оцінку у пацієнток усіх груп дослідження, через 3 місяці після проведеного лікування даний показник покращився у всіх 3-х підгрупах основної групи, з найбільш значущим поліпшенням в Iб та Iв підгрупах на противагу групи порівняння, де цей аналогічний показник в переважній більшості не лише залишився без змін, але й в декількох випадках відмічене погіршення та приєднання нових симптомів.
Висновки. Оцінивши показники суб'єктивної оцінки якості життя жінок та динаміку їх скарг до і після нехірургічної корекції початкових стадій ГП відмічене значне поліпшення якості життя в результаті лікування із застосуванням алгоритму диференційованого підходу в залежності від виду, стадії, супутньої соматичної патології та особливостей морфологічної будови стінки піхви жінок.
Ключові слова: цистецеле, ректоцеле, нехірургічна корекція, якість життя.