bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of low temperature and host plant on Tuta absoluta
Authors: Campos Mateus R. , Amiens‐Desneux Edwige , Béarez Philippe , Soares Marianne A. , Ponti Luigi , Biondi Antonio , Harwood James D. , Desneux Nicolas 
Published: August 2021

AbstractAlternative host plants are among the key factors influencing the spread of invasive pests because they are utilized as a food source and provide shelter in unfavorable conditions. The South American tomato pinworm (SATP), Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), has a high behavioral and physiological plasticity enabling it to rapidly spread in several countries. Among the multiple strategies used by SATP in the invasion process is the use of alternative host plants including black nightshade (BNS), Solanum nigrum L. (Solanaceae), a perennial plant widely distributed across all habitats worldwide. Besides the life table and behavioral parameters of SATP on tomato, its survival and reproduction in low temperatures on alternative host plants should be assessed to evaluate the likely spread in temperate regions with harsh winters. In our study, comparing solanaceous species through generations, the primary difference was in the mean generation time with SATP reared on BNS, whereby it had a longer development time than larvae and pupae reared on tomato plants. Adults preferred tomato plants even if they had been reared as larvae on BNS. Exposure periods of 7, 14, and 21 days to 4 °C indicated that more than 50% of SATP pupae reared on BNS plants survived more than 14 days. The survival of SATP reared on tomato plants exceeded 21 days and after exposure to 4 °C, females reared on both plants remained fertile. The life table and behavioral parameters recorded demonstrated a significant potential of BNS to support the development of SATP, also at low temperature. Therefore, even with effective border surveillance and phytosanitation processes in place, invasion through an alternative host is possible and difficult to detect.
First report of Bactrocera latifrons Hendel in the Democratic Republic of Congo
Authors: Ndayizeye Liévin , Kataraka Balangaliza Clément 
Published: August 2021

AbstractBactrocera latifrons Hendel is an invasive species detected in 2016 in Burundi, a neighbouring country of the Democratic Republic of Congo. An incubation study was carried out over a one‐month period from 15 February to 15 March 2020 on African eggplant (Solanum aethiopicum) collected in Kavimvira, Uvira territory. The results of this study showed that Bactrocera latifrons is present in the eastern part of the Democratic Republic of Congo. Further studies should be conducted on the geographical distribution of Bactrocera latifrons in the different regions of the Democratic Republic of Congo and its host plants.
Impact of endodontic and periodontal diseases and treatments on the aorta and liver of obese and non‐obese rats
Authors: Jara Cynthia Mireya , Pereira Karina Kiimiko Yamashina , Maito Fábio Luiz Dal Moro , Adorno Carlos Gabriel , Gomes Maximiliano Schünke 
Published: August 2021

AbstractAimTo evaluate the impact of the presence and treatment of periodontal disease (PD) and apical periodontitis (AP) on the aorta and liver of obese and non‐obese rats.MethodologyOne hundred and forty Wistar rats were divided into two groups, according to the diet administered: normal diet (‐n), without obesity; and cafeteria diet (‐c), with induced obesity. These groups were divided into seven subgroups according to the specific experimental protocols: naïve control (NC); AP; AP with treatment (APt); PD; PE with treatment (PDt); AP and PD (APPD); and AP and PD with treatment (APPDt). AP and PD lesions were induced for four weeks. Four weeks after treatments, the animals were euthanatized, and the aorta and liver were dissected for histological evaluation. For the comparison of the thickness of the aorta between groups, the Kruskal–Wallis test was used, followed by the Mann–Whitney test. For the analysis of other variables related to the aorta and liver outcomes, logistic regression was carried out.ResultsBoth PD and AP were associated with the development of histological alterations in the aortic arch, with no significant difference between obese and non‐obese animals (p = .17). The aorta thickness was increased significantly (p < .05) with the combination of PD and AP in obese rats (APPDt‐c group) compared with the other groups (NC‐n, APt‐n, APt‐c and AP‐c). The logistic regression models revealed that the untreated (OR = 7.78; 95%CI = 2.4–25) and treated (OR = 2.9; 95%CI = 1.0–8.4) groups were significantly more likely to have endothelial alterations compared with the control groups (p = .002). Obesity (OR = 16.5; 95%CI = 3.4–81.3) was the only predictor variable of liver steatosis (p < .001).ConclusionHistological alterations in the aortic arch of obese and non‐obese rats were observed in the presence of periodontal disease and apical periodontitis. The combination of PD and AP increased the aorta thickness in obese rats. A reduction of vascular endothelial lesions was observed with the treatments of PD and AP.
Opera participants’ perceptions of brand resonance
Authors: Hall Emma , Menzies Jane , Zutshi Ambika , Creed Andrew 
Published: August 2021

AbstractBrand resonance is a marketing concept under‐explored in the performing arts context. This paper responds by analysing focus group findings from a large Opera group, “Big Opera[1]” (For research integrity and anonymity requirements of focus group research, the company pseudonym is Big Opera) on how decision‐makers can enhance brand resonance. Opera patronage was dwindling before the COVID‐19 pandemic, which was further crystallized during the pandemic. As the performing arts sector tries to revive, it is useful to understand how brand resonance concepts can be used to reinvigorate patronage at the opera. A qualitative study of four opera focus groups, which included 24 participants with varying levels of attendance was conducted to examine brand resonance. The study reveals that when customers have brand attachment and love, developed through intense experiences at the opera, facilitated by engagement with the brand, and community, then brand loyalty develops for the opera by intention to re‐purchase. The paper contributes to the literature by making recommendations on how to enhance brand resonance thereby increasing attendance. Intense experiences at the opera and engagement can be enhanced by offering packaged deals, such as accommodation and dinner, or drinks and canapés packages, backstage access, the development of a social club, social media brand community and loyalty programs to increase brand loyalty.
Confocal microscopy, dermoscopy, and histopathology features of atypical intraepidermal melanocytic proliferations associated with evolution to melanoma in situ
Authors: Rocha Lilian K. F. L. , Vilain Ricardo E. , Scolyer Richard A. , Lo Serigne N. , Drummond Martin , Star Phoebe , Fogarty Gerald B. , Hong Angela M. , Guitera Pascale 
Published: August 2021

AbstractBackgroundAtypical intraepidermal melanocytic proliferations (AIMP) is a descriptive term sometimes applied to biopsies that do not fulfill diagnostic criteria of melanoma. They are common on sun‐damaged skin, but their definition and management are controversial.ObjectiveTo describe dermoscopic (DS), reflectance confocal microscopic (RCM) and histopathological features of AIMP and identify features associated with subsequent melanoma in situ (MIS).MethodsA retrospective analysis of AIMP lesions correlated with patient outcome at two melanoma tertiary centers between 2005 and 2015.ResultsThirty‐four patients were included. Nine (26%) patients had MIS in subsequent biopsies. Predictors of later MIS were target‐like pattern (OR:12.0 [CI: 1.23, 117.41]; P = 0.032) and high‐density vascular network (OR:12 [CI: 1.23–117.41], P: 0.032) on DS, and presence of dendritic cells touching each other (OR:9.1 [CI: 1.54, 54.59], P = 0.014) on RCM. Clinical predictors of worse outcome included a previous history of MIS at the same site. Radiotherapy for AIMP had a high failure rate (all patients presented with recurrent disease, three as AIMP and two as MIS).ConclusionsConsidering that most cases in this series received non‐surgical treatment at baseline, we recommend close monitoring for lesions with target‐like pattern and density vascular network on DS and treatment for lesions with progression of atypia and/or with “confluent” dendritic cells on RCM. Although the number of patients in this series is very low, early surgery is recommended for MIS cases that recur as AIMP.
Critical roles of the E3 ubiquitin ligase FBW7 in B‐cell response and the pathogenesis of experimental autoimmune arthritis
Authors: Feng Chunlei , Li Lingyun , Zhou Lei , Li Dali , Liu Mingyao , Han Shuhua , Zheng Biao 
Published: August 2021

AbstractProper regulation of B‐cell function is essential for effective humoral immunity and maintenance of immune tolerance. Here, we found that FBW7 (F‐box/WD40 repeat‐containing protein 7) is highly expressed in germinal centre B and B1 cells, and confirmed that it has an intrinsic role in maintaining homeostasis of mature B cells and B‐1 cells. FBW7 deletion led to an impairment of antibody response, and although germinal centre formation was not affected, antibody class‐switch recombination and affinity maturation processes were defective. Likewise, memory immune response was severely impaired. Moreover, FBW7 ablation ameliorated the pathogenesis of an autoimmune disease model, collagen‐induced arthritis, by reducing the production of anti‐collagen II autoantibodies. Taken together, these data suggest that FBW7 may be an attractive target for developing new therapeutics for the treatment of autoimmune diseases.
Learning Together? What Are, and What Could Be, the Experiences of Adult Participants to ‘Family’ Workshops in an Art Museum
Authors: Ellis Phillipa , King Heather 
Published: August 2021

AbstractMuseums and galleries have long welcomed, and long valued, visiting family groups, and many offer ‘family workshops’. In practice, most workshops are primarily directed at children, and accompanying adults are left to observe. However, we suggest that in actively observing and also pitching in, adults too are engaged in learning. Using the theoretical lens of intent participation, we discuss data from field observations of family workshops and adult focus groups to document the ways in which adults learn while attending child‐focused activities. Over 220 adults and 200 children were observed over a two‐month period attending family workshops in an art museum in London. Findings demonstrate that accompanying parents and carers engaged in the key processes of intent participation: the adults participated in focused and attentive listening, and the detailed watching of others. They also sought new information and actively contributed to the wider output of the family group. We conclude by suggesting that the lens of intent participation, which was initially coined to describe the nature of learning in many traditional, non‐Western, settings, offers designers of family workshops a useful framework for reflecting upon, and thereafter enhancing, the experiences of the adult members of a participating family.
Overexpression of let‐7d explains down‐regulated KDM3A and ENO2 in the pathogenesis of preeclampsia
Authors: Xu Qian , Song Yonghui , Lu Lili 
Published: August 2021

AbstractPre‐eclampsia (PE) is the leading cause of maternal death; however, the causative molecular basis remains largely unknown. Recent studies have revealed the important role microRNAs (miRNAs) play in PE. We aimed to explore the effects of let‐7d on trophoblast proliferation, migration, invasion and apoptosis in PE and its underlying mechanism. Placental tissues were collected from PE patients and healthy pregnant women, and it was found that let‐7d expression was increased, while KDM3A and ENO2 expression was decreased in PE tissues and cells. Bioinformatics analysis indicated the interaction among let‐7d, KDM3A and ENO2, confirmed by dual luciferase reporter gene assay; ChIP experiment identified methylated modification to ENO2 by KDM3A. With gain‐ and loss‐function method, silencing of let‐7d increased KDM3A expression and enhanced the binding between KDM3A and ENO2. Furthermore, overexpression of let‐7d suppressed cell proliferation, migration and invasion of trophoblasts, and induced apoptosis of trophoblasts, while these capacities were restored upon additional treatment of overexpressed ENO2. PE rat models were established to explore the effects of let‐7d and ENO2 on PE in vivo. The results established that the silencing of let‐7d alleviated the tissue injury and PE‐related symptoms when reducing urine protein, TUNEL‐positive cells and increasing ENO2, and KDM3A expression in rats. Cumulatively, let‐7d suppressed cell progression of trophoblasts, and induced apoptosis through the down‐regulation of KDM3A to promote ENO2 methylation, thereby promoting progression of PE. Such an epigenetic network of let‐7d, KDM3A and ENO2 in the pathogenesis of PE might provide novel insight into targeted therapy against this disorder.
The attraction trick: males in early stages of disease become more chemically attractive to Nile tilapia (Oreochromis niloticus) females
Authors: Rossi Vanessa Stramantinoli , de Mello Isabela Maria , Barki Assaf , Giaquinto Percilia Cardoso 
Published: August 2021

AbstractIn aquatic systems, olfaction plays an important role in acquiring information about the social environment and influences important behaviours in various contexts, including predator avoidance, foraging, aggressive and reproductive behaviour and mate selection. As the presence of diseases might modify individual odour, fish may use the variability in conspecifics’ odours as an indicator of the health status and infectious load of potential mates. Here, female Nile tilapia were tested for their ability to detect infected males and discriminate between bacterial infected and uninfected individuals by means of chemical cues. Females were allowed to choose between the odours of males infected by Aeromonas hydrophila bacteria and uninfected males. The findings show that female Nile tilapia initially showed a preference for infected males in terms of their first choice in a dichotomous choice test, but the total duration of time spent with the stimulus from infected males was not longer than that for the uninfected males. This may indicate that males at early stages of infection, i.e., without advanced clinical signs of infection, emit odours that allow them to enjoy the benefits of socialization when the infection is not yet detected by conspecifics. Thus, in the context of reproduction, males might attract female partners and have some chance of reproducing, before being avoided.
Study of the characteristics of facial skin tone status in 1092 young Chinese females according to the ITA°
Authors: Li Nan , Yang Xiao‐Xiao , Yang Ru‐ya , Yi Fan 
Published: August 2021

AbstractBackgroundThe ITA° is the gold standard for skin tone classification. Different skin tones are often associated with different skin characteristics and issues. Different skin types are often associated with different skin characteristics and issues in China.AimsTo study the population's skin color distribution and accompanying skin problems according to the ITA° classification standard.MethodsA total of 1092 women aged 22–42 years were recruited in 7 cities in China. All biophysical parameter measurements (SM, CM, TWEL, pH, R2, GLOSS_DSCT, MEXA, ERYTH, ITA°; 9 indexes total) were quantified with noninvasive instruments. All volunteers provided consent before enrollment.ResultThe main skin color categories were light (II), very light (I), intermediate (III), and tan (IV). The results demonstrated that the characteristics of the facial skin based on the ITA° were significantly different among cities and age groups and were associated with different skin issues.ConclusionsLighter skin was associated with worse skin elasticity; intermediate skin was associated with worse skin hydration content and was most prone to being oily; and darker skin was associated with poor barrier function. Established principal component regression (PCR) indicated that pH, gloss GLOSS_DSC, MEXA, ERYTH, TEWL, and SM had significant effects on the ITA°.
Fluoridated water impact on tooth decay and fluorosis in 17–20‐year‐olds exposed to fluoride toothpaste
Authors: Marques Ravena Brito , Lima Cacilda Castelo Branco , de Abreu Costa Marina Lua Vieira , de Deus Moura de Lima Marina , de Fátima Almeida de Deus Moura Lúcia , Tabchoury Cinthia Pereira Machado , de Moura Marcoeli Silva 
Published: August 2021

AbstractObjectiveTo determine the impact of water fluoridation on the prevalence and severity of dental caries and fluorosis in individuals aged 17–20 years exposed to fluoride toothpaste.MethodsThe study population consisted of 660 students from public schools, residents of areas supplied with fluoridated water (exposed group) or not (not exposed group). Students from both groups had access to fluoride toothpaste throughout life. A questionnaire about socioeconomic demographic aspects, conditions related to access and exposure to fluoridated water, and habits related to oral health was applied. Dental caries was measured by the DMFT index and dental fluorosis by the TF index. The chi‐square test, t test, and subsequently logistic regression were applied for data analysis.ResultsCaries experience (DMFT≠0) was significantly higher in students from areas not exposed to fluoridated water, after adjustments to clinical conditions, demographic socioeconomic profile, and hygiene habits. The DMFT mean (±SD) was significantly higher in students from areas not exposed to fluoridated water than exposed (3.83 [±3.28] and 2.48 [±2.71] respectively). The prevalence of very mild/mild and moderate fluorosis was 41.1% and 21% for students either exposed to fluoridated water or not, respectively.ConclusionExposure to fluoridated water was associated with a lower prevalence and severity of tooth decay, in spite of the use of fluoridated toothpaste. The prevalence of dental fluorosis at all levels was higher in fluoridated areas, however, in both groups, there were few cases with esthetic implications.
Insomnia disorder and long‐term mortality in adult patients treated with extracorporeal membrane oxygenation in South Korea
Authors: Park Hye Yoon , Song In‐Ae , Cho Hyoung‐Won , Oh Tak Kyu 
Published: August 2021

SummaryWe investigated the prevalence of insomnia in extracorporeal membrane oxygenation (ECMO)‐treated patients and examined the association between post‐ECMO insomnia disorder and long‐term mortality. In the present population‐based cohort study, we used data from the National Health Insurance Claims database in South Korea. All adult patients who underwent ECMO between 2006 and 2014 were included, and ECMO‐treated patients were defined as those who survived >365 days after ECMO. Insomnia disorder was identified using the International Classification of Diseases 10th Revision codes G47.0 and F51.0. Overall, 3,055 ECMO‐treated patients were included in the final analysis: 431 (14.1%) had pre‐ECMO insomnia disorder, while 148 (4.8%) were newly diagnosed with insomnia disorder up to 1 year after ECMO. In multivariable Cox regression model, patients with post‐ECMO insomnia disorder had higher 5‐year all‐cause mortality (ACM) than controls (hazard ratio [HR] 1.71, 95% confidence interval [CI] 1.21–2.42; p = 0.003); however, those with pre‐ECMO insomnia disorder did not (p = 0.655). In sensitivity analysis, post‐ECMO insomnia disorder with underlying psychiatric illness was associated with a 2.39‐fold higher 5‐year ACM in ECMO‐treated patients (HR 2.39, 95% CI 1.52–3.75; p < 0.001). In conclusion, at 1‐year after ECMO, 4.8% of ECMO‐treated patients were newly diagnosed with insomnia disorder, and post‐ECMO insomnia disorder was associated with higher 5‐year ACM, especially in those with underlying psychiatric illness. Our present results suggest that the development of insomnia disorder might be related to poorer long‐term survival in ECMO‐treated patients, especially in case of underlying psychiatric illness.
Is serum zinc status related to gestational diabetes mellitus? A meta‐analysis
Authors: Fan Jiehui , Zhang Tingting , Yu Yanchao , Zhang Bao 
Published: August 2021

AbstractGestational diabetes mellitus (GDM) is a common medical disorder that begins during pregnancy. The present work aimed to investigate the relationship of maternal or foetal circulatory zinc levels with GDM. Related studies were retrieved against the PubMed/Medline, Web of Science, Scopus databases till July 2020. The overall effects were expressed as standard mean difference (SMD). Furthermore, the random effects model was used to assess the summarised risk ratios (SRRs) to determine the relationship between zinc and the risk of GDM. A total of 15 articles involving were retrieved for meta‐analysis; in the meantime, 4955 subjects including 1549 GDM cases were enrolled for quantitative analysis. Compared with normal control, GDM cases had decreased circulating zinc level on the whole, but the difference was not statistically significant (SMD = −0.40, 95%CI: −0.80 to −0.00, P = 0.05). Interestingly, upon subgroup analysis stratified by serum zinc content but not plasma zinc concentration, there was significant difference in zinc content between GDM cases and normal controls (SMD = −0.56; 95%CI: −1.07 to −0.04, P = 0.03). Meanwhile, subgroup analysis also revealed similar tendency among the Asians and during the 2nd trimester, but not among the Caucasians or during the 1st or 3rd trimester. Data extracted from four studies that compared pregnant women with GDM in the high level of zinc and GDM in the low level of zinc yielded an SRR of 0.929 (95%CI: 0.905–0.954). According to existing evidence, the serum zinc content decreases among GDM cases compared with subjects with no abnormality in glucose tolerance, in particular among the Asians and during the second trimester. Nonetheless, more well designed prospective study should be carried out for understanding the dynamic relationship of zinc level with the incidence of GDM.
Lymphatic microcirculation profile in the progression of hypertension in spontaneously hypertensive rats
Authors: Wang Bing , Sheng Youming , Li Yuan , Li Bingwei , Zhang Jian , Li Ailing , Liu Mingming , Zhang Honggang , Xiu Ruijuan 
Published: August 2021

AbstractObjectiveThe contractile behavior of collecting lymphatic vessels occurs in essential hypertension in response to homeostasis, suggesting a possible role for microcirculation. We aimed to clarify the nature of the lymphatic microcirculation profile in spontaneously hypertensive rats (SHRs) and normotensive controls.MethodsThe vasomotion of collecting lymphatic vessels in eight‐ and thirteen‐week‐old SHRs and age‐matched Wistar‐Kyoto rats (WKYs, n = 4 per group) was visualized by intravital video and VasTrack. The lymphatic vasomotion profile (frequency and amplitude) and contractile parameters (contraction fraction and total contractility activity index) were compared. Plasma nitrite/nitrate levels were assessed by the Griess reaction, and plasma endothelin‐1 was measured by enzyme‐linked immunosorbent assay.ResultsWKYs and SHRs differed in the vasomotion of collecting lymphatic vessels. Both eight‐ and thirteen‐week‐old WKYs revealed a high‐amplitude pumping pattern, whereas a low‐amplitude pattern was observed in SHRs. Moreover, compared with age‐matched WKYs, SHRs exhibited deteriorated output and reflux capability and lost the ability to regulate collecting lymphatic vasomotion. Additionally, the chemistry complements the microcirculatory lymphatic profile as demonstrated by an increase in plasma nitrite, nitrate, and endothelin‐1 in SHRs. ET‐1 inhibitor meliorated the lymphatic contractile capability in SHRs partially through regulating frequency of lymphatic vasomotion.ConclusionsWe used an intravital lymphatic imaging system to observe that SHRs exhibit an impaired collecting lymphatic vasomotion profile and deteriorated contractility and reflux.