bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical Outcomes of Multiple Robot‐Assisted Hysterectomies in a Single Workday by the Same Surgeon
Authors: Nozaki Takahiro , Matsuda Kosuke , Hosaka Ayaka , Ito Yoshihiko , Kagami Keiko , Sakamoto Ikuko 
Published: December 2024

ABSTRACTIntroductionDue to the growing medical need for gynecologic robotic surgery, several robotic surgeries may be performed in a single day at high‐volume centers. This study evaluated the safety of performing multiple robot‐assisted hysterectomies (RAHs) per day by the same surgeon.MethodsWe reviewed the clinical data of patients who underwent robotic surgery from April 2018 to September 2024 at the Department of Gynecology, Yamanashi Central Hospital, and also examined the surgical type, order, and surgeon for each procedure.ResultsA total of 352 RAHs performed by the same surgeon were included. Among them, 267 were the first and second cases performed on the same day (Group A), and 85 were the third to fifth cases (Group B). There were no statistically significant differences between the two groups regarding age, body mass index, uterine weight, surgical indication, and history of abdominal surgery. The median operative time of 68 (35–179) min in Group A and 66 (37–187) min in Group B was similar (p = 0.141). Both groups also had similar estimated blood loss (p = 0.744). Each group had two perioperative complications, and no patient underwent conversion to open or laparoscopic surgery.ConclusionPerforming multiple RAHs by the same surgeon in a single day may be a safe procedure with no negative impact on operative time, blood loss, or perioperative complications. Hence, it could be a useful treatment option for high‐volume centers.
Trait responsiveness to verbal suggestions predicts nocebo responding: A meta‐analysis
Authors: Stein Madeline V. , Heller Monika , Chapman Sarah , Rubin G. James , Terhune Devin B. 
Published: December 2024

AbstractBackgroundNocebo responding involves the experience of adverse health outcomes in response to contextual cues. These deleterious responses impact numerous features of mental and physical health but are characterized by pronounced heterogeneity. Suggestion is widely recognized as a contributing factor to nocebo responding but the moderating role of trait responsiveness to verbal suggestions (suggestibility) in nocebo responding remains poorly understood.ObjectiveWe conducted a pre‐registered meta‐analysis (PROSPERO registration number CRD42023425605) to quantitatively synthesize available research on the relationship between suggestibility and nocebo responding.MethodsFour electronic databases were searched for original studies involving both the assessment of suggestibility and symptom reports in response to an inactive stimulus.ResultsOf 7729 search results, 10 articles presenting 13 correlations between suggestibility and nocebo responding were analysed. A random‐effects meta‐analysis revealed a significant, albeit weak, positive correlation, r = .21 [95% CI: .04, .37], between suggestibility and nocebo responses, such that more highly suggestible individuals displayed larger responses. Sensitivity and meta‐regression analyses demonstrated that studies of higher methodological quality, including those that maintained experimenter blinding, exhibited stronger effect sizes.ConclusionThese results corroborate proposals that trait responsiveness to verbal suggestions confers greater response to nocebos and warrants renewed attention to the role of suggestibility in symptom induction and perception.
Simulated Impacts of Harvesting Chamaedorea linearis and C. pinnatifrons (Arecaceae): Implications for Their Conservation
Authors: Cepeda Paula , Balslev Henrik , Isaza Carolina 
Published: December 2024

ABSTRACTPalms are important sources of nontimber forest products. One of their most common uses is as ornamentals, which often involves harvesting whole individuals or plant parts from wild populations. Chamaedorea linearis and Chamaedorea pinnatifrons are still not excessively harvested, but they have substantial ornamental potential, and their populations are decreasing. The use of a wild species can contribute to its conservation if the use is governed by adequate harvest rates. We simulated harvest impacts on the population dynamics of C. linearis and C. pinnatifrons in an Andean montane forest in Parque Natural Chicaque, Colombia, using integral projection models (IPMs) over the period 2019–2021. We projected management scenarios including the harvest of juveniles, as well as leaves and fruits of adults. In our model, the finite growth rate of C. linearis decreased (λ = 0.76–0.91) whereas in C. pinnatifrons it remained stable (λ = 0.98–1.04). The simulations of the harvest of juveniles and leaves suggested negative impacts on the populations' sizes and in the transient growth rate (λ20) in the long term when compared to no harvest. Fruits harvest had no considerable effects under 20% of removal once per year in both of the populations, but had a decrease in populations under 20% of removal twice and four times a year. We conclude that sustainable use strategies should focus on protecting juveniles in both populations. Fruit harvest for propagation can be promoted as a sustainable use strategy that does not endanger their conservation.
Intraoperative Cardiac Arrests in Asian Recipients of Liver Transplantation—Second Report After Learning Curve
Authors: Choi Jisun , Lee SangHyun , Ko Justin Sangwook , Gwak Mi Sook , Kim Gaab Soo 
Published: December 2024

ABSTRACTBackgroundAlthough surgical competency and anesthesia for liver transplantation (LT) have evolved significantly in the past decades, intraoperative cardiac arrest (ICA) is still an event that brings a poor prognosis to the recipient. We report a second‐decade experience of ICA as a follow‐up study of our first report at our institution.MethodsThis is a retrospective observational study of the medical records and the Liver Transplant Program database of our institution. LT from January 2011 to June 2023 were included. Of the 1735 LT cases, a total of 1730 cases were included, excluding three non‐Asian and two simultaneous heart and liver transplants (1598 adult LT, 132 pediatric LT).ResultsThe ICA incidence during adult LT was 0.7% (11/1598) which is significantly lower compared to our first report (1.5%; 14/919) (p = 0.042). ICA occurred only in adult recipients. Post‐reperfusion syndrome (PRS, six cases) and bleeding (four cases) were the primary causes in most cases and most ICA occurred after reperfusion (10/11). The mortality rates within 24 h, 30 days, and 1 year were 27.3%, 45.5%, and 54.5%, respectively. The survival curve did not show a significant difference from our first report (p = 0.570), and the survival rate of the ICA group was significantly lower compared to the non‐ICA group. (p = 0.000)ConclusionThe incidence of ICA has decreased, but the main causes of ICA as PRS and bleeding after reperfusion have not changed. Additionally, there was no significant difference in the survival curves from the first report. Because ICA is still fatal, efforts to reduce its incidence should be continued.
Mitral Regurgitation Evaluation in Modern Echocardiography: Bridging Standard Techniques and Advanced Tools for Enhanced Assessment
Authors: Leo Laura Anna , Viani Giacomo , Schlossbauer Susanne , Bertola Sebastiano , Valotta Amabile , Crosio Stephanie , Pasini Matteo , Caretta Alessandro 
Published: December 2024

ABSTRACTMitral regurgitation (MR) is one of the most common valvular heart diseases worldwide. Echocardiography remains the first line and most effective imaging modality for the diagnosis of mitral valve (MV) pathology and quantitative assessment of MR. The advent of three‐dimensional echocardiography has significantly enhanced the evaluation of MV anatomy and function. Furthermore, recent advancements in cardiovascular imaging software have emerged as step‐forward tools, providing a powerful support for acquisition, analysis, and interpretation of cardiac ultrasound images in the context of MR. This review aims to provide an overview of the contemporary workflow for echocardiographic assessment of MR, encompassing standard echocardiographic techniques and the integration of semiautomated and automated ultrasound solutions. These novel approaches include advancements in segmentation, phenotyping, morphological quantification, functional grading, and chamber quantification.
Dorsolateral pontine lesions produce distinct ocular motor abnormalities with anatomical correlations
Authors: Kim Hyun Sung , Choi Jae‐Hwan , Oh Eun Hye , Choi Seo Young , Choi Kwang‐Dong 
Published: December 2024

AbstractBackground and PurposeThe dorsolateral portion of the caudal pons contains the vestibular nucleus (VN) and inferior cerebellar peduncle (ICP) that play important roles in conveying and processing vestibular and ocular motor signals. This study aimed to characterize ocular motor abnormalities along with their anatomical correlations in dorsolateral pons (DLP) lesions.MethodsWe analyzed clinical features, and results of neuro‐otological evaluations and neuroimaging of 18 patients with unilateral DLP lesions (17 with DLP infarction and 1 with cavernous malformation) from among 506 patients with pontine infarction in a stroke registry.ResultsMost of the patients (n = 16) presented with isolated acute vestibular syndrome (AVS). The involved structures within the DLP were the ICP in four patients and the VN in the remaining 14. The unilateral ICP lesions were associated with consistent abnormalities in vestibular and ocular motor tests, including ipsilesional nystagmus without gaze‐evoked nystagmus (GEN), and normal head impulse tests (HITs) and caloric response. In contrast, lesions of the VN were associated with a broader range of eye‐movement abnormalities, including ipsi‐ or contralesional nystagmus with GEN, positive HITs, normal or abnormal caloric responses, and fixation nystagmus. Initial diffusion‐weighted magnetic resonance imaging (within 48 h) was falsely negative in 41% (n = 7) of the DLP infarction cases.ConclusionsThis study demonstrates that unilateral DLP lesions frequently present with isolated AVS and diverse ocular motor abnormalities. These characteristics may be due to the complex involvement of afferent or efferent fibers to and from the VN.
Co‐Investments in Private Equity: Do Investor Types Matter?
Authors: Flor Christian Riis , Jensen Emil Tilgaard , Schandlbauer Alexander 
Published: December 2024

ABSTRACTThis paper utilizes detailed data from a Northern European private equity fund‐of‐funds to examine the determinants of co‐investments. Unlike prior studies, we differentiate between two distinct types of co‐investors: sidecar co‐investors and direct co‐investors. This distinction matters. Both types are more likely to participate in larger deals, but the effect is notably stronger for sidecar co‐investments. Furthermore, if the internal funds available to a general partner (GP) are less than the initial capital used for the acquisition, a GP is more inclined to seek sidecar co‐investors, while lower uncalled capital reduces the likelihood of co‐investment offers.
Strategies to Guide Paediatric Type 1 Diabetes Management and Transition of Care: A State‐Of‐The‐Art Review
Authors: Pehr‐Szeliga Danielle , Rutkowski Aneta , Neuman Michelle , Amer Kim , Webber‐Ritchey Kashica J. 
Published: December 2024

ABSTRACTAimThe aim of the study was to synthesise current evidence on the transition of type 1 diabetes management responsibility from caregiver to child.DesignA state‐of‐the‐art review was conducted.MethodsUsing Rayyan software, two authors independently performed the study selection.Data SourcesCINAHL, PubMed and Web of Science databases were searched to identify relevant articles that focused on children ages 1–19 who were transitioning care of paediatric type 1 diabetes. The last search was performed on 17 October 2023.ResultsThirty‐seven studies conducted in four different countries were included following abstract screening, full‐text review and assessment for risk of bias—23 quantitative, 13 qualitative and one mixed method. One study examined early childhood transition, five studies examined middle childhood and 31 studies examined transition strategies utilised in later childhood. Themes in clinician and caregiver transition interventions were identified.ConclusionFindings can inform the development and refinement of transition interventions to help clinicians and caregivers ensure a successful transition of type 1 diabetes management to the paediatric patient.Implications for the Profession and Patient CareA rigorous investigation to validate strategies for different age groups is needed. To support this process and improve type 1 diabetes mellitus outcomes for paediatric patients, research and recommendations should be further reviewed to assist in tailoring interventions accordingly.Impact StatementEffective transition management strategies to shift responsibility from caregiver to child are increasingly important to ensuring safe self‐management of paediatric type 1 diabetes. Reviews on type 1 diabetes transition management strategies for youth are needed. In this systematic review, we highlight transition strategies for children ages 1–19 who were transitioning care of paediatric type 1 diabetes. Findings offer insight on long‐term diabetes management to improve patient outcomes in paediatric patients with type 1 diabetes.Reporting MethodWe adhered to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines.Patient or Public ContributionNo patient or public contribution was required in this review.
Deciphering the Significance of Platelet‐Derived Chloride Ion Channel Gene (BEST3) Through Platelet‐Related Subtypes Mining for Non‐Small Cell Lung Cancer
Authors: Ren Hanxiao , Du Meng‐Ze , Liao Yulin , Zu Ruiling , Rao Lubei , Xiang Run , Zhang Xingmei , Liu Shan , Zhang Peiyin , Leng Ping , Qi Ling , Luo Huaichao 
Published: December 2024

ABSTRACTThis study investigates platelet‐related subtypes in non‐small cell lung cancer (NSCLC) and seeks to identify genes associated with prognosis, focusing on the clinical significance of the chloride ion channel gene BEST3. We utilised sequencing and clinical data from GEO, TCGA and the Xena platform, building a risk model based on genetic features. TCGA and GSE37745 served as training cohorts, while GSE50081, GSE13213, GSE30129 and GSE42127 were validation cohorts. Immunotherapy datasets (GSE135222, TCGA‐SKCM) were also analysed. Differentially expressed genes (DEGs) were identified using Limma, subtypes through ConsensusClusterPlus and key prognostic genes using COX regression, Random Forest and LASSO‐COX. BEST3 expression was validated by flow cytometry (FCM) and functional assays in A549 cells with lentiviral overexpression evaluated its impact on apoptosis, proliferation and migration. Three platelet‐related subtypes were identified, with ten key prognostic genes (including BEST3). Gene Ontology (GO) analysis showed six genes involved in platelet pathways. BEST3 was highly expressed in the platelet subtype 1. Flow cytometry confirmed elevated BEST3 levels in NSCLC (35.9% vs. 27.3% in healthy individuals). Overexpression of BEST3 in NSCLC cells suppressed apoptosis and promoted proliferation and migration. The discovery of three platelet subtypes and the role of BEST3 in promoting tumour growth and migration highlights its potential as a therapeutic target and prognostic marker in NSCLC.
Epigenomic Landscape of Human Cumulus Cells in Premature Ovarian Insufficiency Using Single‐Base Resolution Methylome and Hydroxymethylome
Authors: Shi Wenhao , Wang Dongyang , Xue Xia , Qiao Sen , Zhang Wei , Shi Juanzi , Huang Chen 
Published: December 2024

ABSTRACTPremature ovarian insufficiency (POI) has recently been reported to be linked with epigenetic changes. Previous studies have focused on the regulation of individual genes associated with ovarian function through single‐gene epigenetic variations; however, there is a deficiency in the comprehensive comprehension of the epigenetic profile for POI. Therefore, we conducted a multi‐omics study integrating methylation, hydroxymethylation and transcriptome sequencing analyses in cumulus cells from women with POI and their matched controls. Our data revealed significant global increases in methylation and hydroxymethylation levels in POI patients. We observed a predominance of hypermethylated and hyperhydroxymethylated regions across the genome, with methylation in gene bodies negatively correlating with gene expression, especially in promoter regions. Subsequent experimental validation was performed to confirm the involvement of candidate genes (EGR1, EGR2 and DLX5) in ovarian steroid hormone synthesis. Interestingly, our findings indicate that these epigenetic modifications are associated with genes implicated in POI, ovarian function and the epigenetic age clock. This comprehensive epigenetic profile underscores the potential for identifying novel biomarkers and therapeutic targets for POI by unravelling the complex interplay between DNA epigenetics and ovarian function.
Synergistic Potential of Argentatins A and B to Improve 5‐Fluorouracil Cytotoxicity in Colorectal Cancer Cell Models
Authors: Sánchez‐Olivares Paula , Silva‐Nolasco Aniela M. , de la Cruz‐Morcillo Miguel A. , García‐Martínez María Mercedes , Pinedo‐Serrano Alejandro , Carmona Manuel , Galán‐Moya Eva M. 
Published: December 2024

ABSTRACTColorectal cancer is the third most commonly diagnosed cancer worldwide and the second most common cause of cancer‐related death in both men and women. Although a number of treatments are available to combat this malignancy, the antimetabolite 5‐fluorouracil has been the cornerstone of therapy since its synthesis in the 1950s. Unfortunately, the prolonged use of 5‐fluorouracil can lead to chemoresistance, which has prompted research into combination regimens to improve efficacy and quality of life and reduce resistance. Here, we evaluated the synergistic potential of two compounds isolated from guayule, and argentatins A and B, alone and in combination with 5‐fluorouracil in a panel of colorectal cancer cell lines. Cell viability assays showed that the combination treatment (argentatin A with 5 fluorouracil) significantly enhanced cytotoxicity, especially in RKO, where the analysis using the Bliss independence model indicated a remarkable synergistic effect with the lowest doses of both compounds. In contrast to the combination with argentatin B, in which the additive effect was only found in the HCT‐116 cell line. Finally, immunocytometric analysis revealed that combination treatments induced higher rates of apoptosis than single‐agent treatments. Collectively, our findings indicate that argentatins A and B may enhance the anti‐tumour effects of 5‐fluorouracil and may represent a promising strategy to improve the efficacy of anticancer therapies based on this antimetabolite.
The New Tumour Biomarker miRNA‐371‐3p Influences Cisplatin Sensitivity of Testicular Germ Cell Tumour Cell Lines
Authors: Weiten Richard , Engler Theadora , Schorle Hubert , Ellinger Jörg , Saponaro Miriam , Alajati Abdullah , Nettersheim Daniel , Syring‐Schmandke Isabella 
Published: December 2024

ABSTRACTCisplatin is used to treat a variety of malignancies, including testicular germ cell tumours (TGCTs). Although cisplatin‐based chemotherapy yields high response rates, a subset of patients develop cisplatin resistance, limiting treatment options and worsening prognosis. Therefore, there is a high clinical need for new therapeutic strategies targeting cisplatin‐resistant TGCTs. MicroRNA‐371a‐3p (miR‐371), the new serum biomarker for TGCTs, shows significantly increased expression in cisplatin‐resistant TGCT cell lines compared to sensitive parental cell lines. However, the functional impact of miR‐371 on cisplatin sensitivity has not been investigated yet. To evaluate the impact of miR‐371 on cisplatin sensitivity, antagomirs were used to inhibit miR‐371 expression, resulting in a > 98% decrease in miR‐371 expression. Cisplatin sensitivity was significantly increased after miR‐371 inhibition in cisplatin‐resistant and corresponding parental TGCT cell lines, indicating a strongly reduced viability and increased apoptosis after cisplatin treatment in miR‐371‐inhibited cells. Our results suggest that miR‐371 may contribute to the development of cisplatin resistance in TGCTs. Interfering with miR‐371 expression can increase the cisplatin sensitivity of tumour cells, which may represent a promising approach to improve future therapeutic outcomes in patients with TGCTs, especially those with cisplatin‐resistant disease.
Patient‐Reported Outcome Scale for Idiopathic Pulmonary Fibrosis: Development and Validation in China
Authors: Xie Yang , Zhang Peng , Ren Jiaming , Chen Tao , Wang Jiajia , Li Jiansheng 
Published: December 2024

ABSTRACTPurposeTo develop and validate the patient‐reported outcome scale for idiopathic pulmonary fibrosis (IPF‐PRO) to provide a reliable and scientific measure for clinical trials on idiopathic pulmonary fibrosis (IPF).MethodsWe analyzed the relevant literature and medical records and conducted interviews and panel discussions to develop the conceptual framework and generate the item pool. We subjected the collected items to removal, mergence, or modification to form the initial scale through a qualitative review by experts and patients. Subsequently, we conducted two field surveys to select items for the final scale based on the classical test theory and item response theory (IRT). Finally, we conducted a formal survey to assess the measurement properties of the IPF‐PRO.ResultsThe IPF‐PRO included 18 items across four domains, namely physiology, psychology, environment, and satisfaction. The Cronbach's α coefficient and generalized coefficient of the IPF‐PRO were 0.917 and 0.931, respectively. The content validity, structural validity, criterion validity, and discriminant validity all met relevant standards. The results of the item analysis based on IRT were considered acceptable. The ordinal logistic regression analysis findings showed that all items' p values were greater than 0.01 when the domain scores matched variables. The IPF‐PRO response and completion rates were both 100%. The median completion time was 7 min [IRQ = 3.7 min (Q3 = 9.0 min, Q1 = 5.3 min)].ConclusionThe 18‐item IPF‐PRO developed in this study has demonstrated good reliability and validity, indicating that it is a reliable and scientific measure for IPF clinical trials.