bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-disclosing youth: a cross sectional study to understand why young people do not disclose suicidal thoughts to their mental health professional.
Authors: McGillivray Lauren , Rheinberger Demee , Wang Jessica , Burnett Alexander , Torok Michelle 
Published: September 2021

Abstract
Background
Prevalence of suicidal ideation increases rapidly in adolescence, and many choose not to seek help and disclose their ideation. Young people who do disclose suicidal ideation, prefer to do so with peers and family compared to mental health professionals, who are best placed to provide evidence-based treatment. This study aimed to identify key factors associated with young people’s decision to, or not to disclose suicidal thoughts to their mental health practitioner.
Methods
A community-based sample of young Australians (16–25 years), who had experienced suicidal ideation and engaged with a mental health professional, completed an online questionnaire (N = 513) which assessed demographic characteristics, severity of depression, anxiety, psychological distress, and suicidal ideation, lifetime suicide attempts, exposure to suicide loss, personal suicide stigma, prioritisation of mental health issues, and therapeutic alliance. Logistic regression analyses were used to identify factors associated with disclosure.
Results
Though the full sample had engaged in therapy, 39% had never disclosed suicidal ideation to their clinician. Those who had disclosed were more likely to report greater prioritisation of suicidal ideation (OR = 4.07, 95% CI = 2.34–7.09), therapeutic alliance (OR = 1.04, 95% CI = 1.02–1.06), and personal suicide stigma (OR = 1.04, 95% CI = 1.01–1.06). The most common reason for not disclosing was concern that it would not remain confidential.
Conclusion
These findings provide new insights into why young people may not seek help for suicidal ideation, despite being engaged with a mental health professional, and establish evidence to inform practice decisions and the development of prevention strategies to support young people for suicide.
Upset Protrusion Joining (UPJ) Characteristics of Cast AM60 Magnesium Alloy to Join With Dissimilar Material
Authors: Andreae Nicholas , Chalasani Dharmendra , Jain Mukesh K. 
Published: September 2021

Abstract
Magnesium alloys have a significant benefit over the steel and aluminum alloys in manufacturing of components for many automotive and structural applications because of their extreme lightweight, low density, and high strength to weight ratio. However, one of the glaring impediments to the success of steels, aluminum, and magnesium-based multi-material integration for automotive industries is the ability to join these materials together without any cracking and corrosive damages during a performance. The present work aims to demonstrate a cost-effective, novel, and versatile joining technique, named Upset Protrusion Joining (UPJ), to mechanically and rapidly (1-2 seconds) join die-cast AM60 alloy to aluminum alloy sheet and evaluate its UPJ characteristics. Cast Mg plate has a cylindrical protrusion (11 mm diameter and 14 mm height) emanated perpendicular to its flat surface, and an aluminum sheet has a hole that accommodates the protrusion. Mg and Al alloy components are then clamped together, electrically heated, and compressed perpendicular to the protrusion axis. During compression, the protrusion expanded circumferentially to fill the hole as well as the region above the hole, and entrapped the Al sheet between the deformed (in a mushroom shape) head and the Mg plate. The effect of different UPJ process parameters such as applied current, current duration, compression loading rate, and compression distance is studied. The process demonstrated repeatability at given process conditions, and optimum process parameters were identified that produce visibly good joints (defect-free) and sufficient joint strengths when tested in the lap-shear mode under uniaxial tension. AM60 alloy showed a great promise as a candidate alloy to suit the UPJ method to adapt to automotive and other industrial manufacturing units to join with dissimilar wrought Al alloy sheets.
Robotic Transcranial Doppler in Non-Traumatic Subarachnoid Hemorrhage: A Safety and Efficacy Study
Authors: Clare Kevin , Stein Alan , Damodara Nitesh , Feldstein Eric , Alshammari Hussein , Ali Syed , Kurian Christeena , Rosenberg Jon , Bauerschmidt Andrew , Kaur Gurmeen , Santarelli Justin , Hamilton Robert , Mayer Stephan , Gandhi Chirag D. , Al-Mufti Fawaz 
Published: September 2021

Abstract
Introduction: Delayed cerebral ischemia (DCI) secondary to vasospasm is a determinate of outcomes following non-traumatic subarachnoid hemorrhage (SAH). SAH patients are monitored using transcranial doppler (TCD) to measure cerebral blood flow velocities (CBFv). However, the accuracy and precision of manually acquired TCD can be operator dependent. The NovaGuide robotic TCD system attempts to standardize acquisition. This investigation evaluated the safety and efficacy of the NovaGuide system in SAH patients in a Neuro ICU.Methods: We retrospectively identified 48 NovaGuide scans conducted on SAH patients. Mean and maximum middle cerebral artery (MCA) CBFv were obtained from the NovaGuide and the level of agreement between CBFv and computed tomography angiography (CTA) for vasospasm was determined. Safety of NovaGuide acquisition of CBFv was evaluated based on number of complications with central venous lines (CVL) and external ventricular drains (EVD) Results: There was significant agreement between the NovaGuide and CTA (Cohen’s Kappa = 0.74) when maximum MCA CBFv ≥120 cm/s was the threshold for vasospasm. 27/48 scans were carried out with CVLs and EVDs present without negative outcomes. Conclusions: The lack of adverse events associated with EVDs/CVLs and the strong congruence between maximal MCA CBFv and CTA illustrates the diagnostic utility of the NovaGuide.
Title: Comparison of Test-Retest Reliability of Pseudo-continuous Arterial Spin Labelling (pCASL) and BOLD fMRI in a Multi-Site Study
Authors: Ibinson James W. , Gillman Andrea G. , Schmidthorst Vince , Li Conrad , Napadow Vitaly , Loggia Marco L. , Wasan Ajay D. 
Published: September 2021

Abstract
Background: The establishment of test-retest reliability and reproducibility (TRR) is an important part of validating any research tool, including functional magnetic resonance imaging (fMRI). The primary objective of this study is to investigate the reliability of pseudo-Continuous Arterial Spin Labeling (pCASL) and Blood Oxygen Level Dependent (BOLD) fMRI data acquired across two different scanners in a sample of healthy adults. While single site/single scanner studies have shown acceptable repeatability, TRR of both in a practical multisite study occurring in two facilities spread out across the country with weeks to months between scans is critically needed.Methods: Ten subjects were imaged with similar 3T MRI scanners at the University of Pittsburgh and Massachusetts General Hospital. Finger-tapping and Resting-state data were acquired for both techniques. Analysis of the resting state data for functional connectivity was performed with the Functional Connectivity Toolbox, while analysis of the finger tapping data was accomplished with FSL. pCASL Blood flow data was generated using AST Toolbox. Activated areas and networks were identified via pre-defined atlases and dual-regression techniques. Analysis for TRR was conducted by comparing pCASL and BOLD images in terms of Intraclass correlation coefficients, Dice Similarity Coefficients, and repeated measures ANOVA.Results: Both BOLD and pCASL scans showed strong activation and correlation between the two locations for the finger tapping tasks. Functional connectivity analyses identified elements of the default mode network in all resting scans at both locations. Multivariate repeated measures ANOVA showed significant variability between subjects, but no significant variability for location. Global CBF was very similar between the two scanning locations, and repeated measures ANOVA showed no significant differences between the two scanning locations.Conclusions: The results of this study show that when similar scanner hardware and software is coupled with identical data analysis protocols, consistent and reproducible functional brain images can be acquired across sites. The variability seen in the activation maps is greater for pCASL versus BOLD images, as expected, however groups maps are remarkably similar despite the low number of subjects. This demonstrates that multi-site fMRI studies of task-based and resting state brain activity is feasible.
Natural History of Radiological Changes on CT Chest from COVID-19 Infection Rehabilitation Patients
Authors: Peng Ann 
Published: September 2021

Abstract
Object： To investigate the chest CT manifestations of convalescent patients with COVID-19 in recovery phase. Methods: 118 convalescent patients diagnosed as COVID-19 were followed up. According to their medical history, they were divided into 47 cases of severe disease group and 71 cases of mild disease group. Multi-slice spiral CT, high-resolution CT and pulmonary function were examined. Results: 67 rehabilitated cases are normal on CT scan. The other CT findings were: (1) ground glass opacity lesions, including: localized patchy ground glass density lesions; Multi lobes and multi-stage ground glass opacities; Diffuse ground glass opacities lesions in both lungs. (2) The interlobular interstitium and interlobular septum were thickened. (3) Subpleural arc shadow/Subpleural lines. (4) Irregular fiber cord shadow/Irregular lines. (5) Tractive bronchiectasis. (6) Nodular consolidation of air space. (7) Cavitary lesions. No obvious mediastinal lymph node enlargement and pleural effusion were found. Pulmonary imaging and pulmonary function were improved after repeated reexamination. There was significant difference in CT findings and pulmonary function indexes between severe group and mild group (P < 0.05). Conclusion: The pulmonary manifestations of some convalescent patients with COVID-19 are basically normal. Ground glass density lesions are the main CT manifestations of convalescent patients, accompanied by varying degrees of pulmonary interstitial hyperplasia. The severe group had more severe pulmonary manifestations and poor pulmonary function than the mild group. With the extension of time, pulmonary lesions and pulmonary function gradually improved.
Engineering for an HPV 9-valent Vaccine using Genomic Constitutive Over-expression and Low Lipopolysaccharide Levels in Escherichia Coli Cells
Authors: Wang Kaihang , Zhou Lizhi , Chen Tingting , Li Qiong , Li Jiajia , Liu Liqin , Li Yuqian , Sun Jie , Li Tingting , Wang Yingbin , Kong Zhibo , Zheng Qingbing , Zhang Jun , Yu Hai , Gu Ying , Xia Ningshao , Li Shaowei 
Published: September 2021

Abstract
BackgroundThe various advantages associated with the growth properties of Escherichia coli have justified their use in the production of genetically engineered vaccines. However, endotoxin contamination, plasmid vector instability, and the requirement for antibiotic supplementation are frequent bottlenecks in the successful production of recombinant proteins that are safe for industrial-scaled applications. To overcome these drawbacks, we focused on interrupting the expression of several key genes involved in the synthesis of lipopolysaccharide (LPS), an endotoxin frequently responsible for toxicity in recombinant proteins, to eliminate endotoxin contamination and produce better recombinant proteins with E. coli.ResultsOf 8 potential target genes associated with LPS synthesis, we successfully constructed 7 LPS biosynthesis-defective recombinant strains to reduce the production of LPS. The endotoxin residue in the protein products from these modified E. coli strains were about two orders of magnitude lower than that produced by the wild-type strain. Further, we found that 6 loci—lpxM, lpxP, lpxL, eptA, gutQ and kdsD­—were suitable for chromosomal integrated expression of HPV L1 protein. We found that a single copy of the expression cassette conferred stable expression during long-term antibiotic-free cultivation as compared with the more variable protein production from plasmid-based expression. In large-scale fermentation, we found that recombinant strains bearing 3 to 5 copies of the expression cassette had 1.5- to 2-fold higher overall expression along with lower endotoxin levels as compared with the parental ER2566 strain. Finally, we engineered and constructed 9 recombinant E. coli strains for the later production of an HPV 9-valent capsid protein with desirable purity, VLP morphology, and antigenicity. ConclusionReengineering the LPS synthesis loci in the E. coli ER2566 strain through chromosomal integration of expression cassettes has potential uses for the production of a 9-valent HPV vaccine candidate, with markedly reduced residual endotoxin levels. Our results offer a new strategy for recombinant E. coli strain construction, engineering, and the development of suitable recombinant protein drugs.
The effect of Fracture Inclination in Multiple Planes on Fracture Stability and Reoperation Risks in Femoral-Neck Fractures of Nongeriatric Patients
Authors: Jiang Dajun , Zhan Shi , Hu Hai , Zhu Hongyi , Zhang Changqing , Jia Weitao 
Published: September 2021

Abstract
BackgroundFor nongeriatric patients with femoral neck fractures (FNFs), internal fixation treatment is still the primary choice. Preoperative evaluation of fracture inclination and underlying stability is essential for selecting appropriate fixation strategies, thus improving clinical prognoses. The purpose of this study was to comprehensively investigate the 3-D inclination angle in both vertical and oblique planes from CT images, and its association with fracture stability and reoperation risks.MethodsWe retrospectively reviewed the medical records of 755 FNFs patients with over two years follow-up. The 3-D inclination angle in vertical (α) and oblique plane (β) were measured based on CT images. The optimal threshold for unstable 3-D inclination were identified by seeking the highest Youden Index in predicting reoperation and validated in the biomechanical test. According to the cut-off value proposed in the diagnostic analysis, forty-two bone models were divided into seven groups, and were all fixed with traditional three parallel screws. Interfragmentary motion (IFM) was used for comparison among seven groups. The association between reoperation outcome and 3-D inclination was analysed with a multivariate model.ResultsThe overall reoperation rate was 13.2%. Unstable 3-D inclination angles with an optimally determined Youden index (0.39) included α>70°; 50°<α<70° and β>20°/ β<-20°. Biomechanical validation showed these fractures had significantly greater (p<0.05) interfragmentary motion (1.374-2.387mm vs. 0.330-0.681 mm). The reoperation rate in 3-D unstable group (32.7%) is significantly (p<0.001) higher than that in 3-D stable group (7.9%). Multivariate analysis demonstrated that 3-D inclination angle was significantly (OR=4.699, p<0.001) associated with reoperation. Conclusions﻿FNFs with α>70°; 50°<α<70° and β>20°/ β<-20° are real unstable types with significantly worse interfragmentary stability and higher reoperation risks. Fracture inclination in multiple planes is closely related to reoperation outcomes and may enhance the way FNFs are currently evaluated.
Deciphering Genetic Alterations of Taiwanese Pancreatic Adenocarcinoma With Targeted Sequencing
Authors: Huang Chi-Cheng , Liu Chih-Yi , Huang Chi-Jung , Hsu Yao-Chun , Lien Heng-Hui , Wong Jia-Uei , Tai Feng-Chuan , Ku Wen-Hui , Hung Chi-Feng , Lin Jaw-Town , Huang Ching-Shui , Chiang Han-Sun 
Published: September 2021

Abstract
Purpose Pancreatic adenocarcinoma (PAC) is the 8th leading cause of cancer-related death in Taiwan, and its incidence is increasing. The development of PAC involves successive accumulation of multiple genetic alternations. Understanding the molecular pathogenesis and heterogeneity of PAC may facilitate personalized treatment for PAC and identify therapeutic agents.Materials and Methods We performed next-generation sequencing (NGS) with targeted panels to explore the molecular changes underlying PAC in Taiwan. The Ion Torrent Oncomine Comprehensive Panel (OCP) was used for PAC metastatic lesions, and more PAC samples were sequenced with the Ion AmpliSeq Cancer Hot Spot (CHP) v2 panel.ResultsFive fresh-frozen paraffin-embedded (FFPE) specimens were successfully assayed with the OCP, and KRAS was the most prevalent alternation, which might contraindicate the use of anti-EGFR therapy. One PAC patient harbored a FGFR2 p.C382R mutation, who might derive benefit from FGFR tyrosine kinase inhibitor. Additional 38 samples assayed with CHP v2 showed 113 hotspot variants, corresponding to 54 COSMID IDs. The most frequently mutated genes were TP53, KRAS, HRAS, and PDGFRA, (29, 23, 14, 10 hotspot variants), impacting 11, 23, 14, and 10 PAC patients. Highly pathogenic variants including COSM22413 (PDGFRA, FATHMM predicted score: 0.88), COSM520, COSM521, COSM518 (KRAS, FATHMM predicted score: 0.98) were reported.ConclusionsBy using NGS with targeted panel, somatic mutations with therapeutic potential were identified. The combination of clinical and genetic information is useful for decision making and precise selection of targeted medicine.
Ultrahigh-temperature film capacitors via homo/heterogeneous interfaces
Authors: Liu Ming , Lu Rui , Shen Zhonghui , Ma Chunrui , Duan Tingzhi , Lu Lu , Hu Guangliang , Hu Tian-Yi , You Caiyin , Mi Shaobo , Jia Chun-Lin , Chen Long-Qing 
Published: September 2021

Abstract
High-performance dielectric capacitors are in high demand for advanced electronics and electric power systems. They possess high power density (on the order of Megawatt) and exhibit ultrafast charge/discharge capability (on a microsecond scale) and long-term storage lifetime1-5, and thus they are particularly demanded in pulse power systems such as high-power microwaves, hybrid electric vehicles, and high-frequency inverters. However, their relatively low operating temperature limits their widespread applications6-9. Here, guided by phase-field simulations, we synthesized capacitors with an energy storage density of 55.4 joules per cubic centimeter, energy efficiency of over 82%, and superior thermal stability and fatigue properties at record high operating temperature of 400°C. These ultrahigh-temperature performances are achieved through a relatively simple method of introduction and engineering of interfaces within the capacitors, which greatly improve their high-temperature stability, relaxation behavior, and breakdown strength. Our work not only successfully fabricated capacitors with potential applications in high-temperature electric power systems and electronic technologies but also opens up a promising and general route for designing high-performance electrostatic capacitors through interface engineering.
Waist Circumference and Mortality or Cardiovascular Events in a General Korean Population
Authors: Song Do Kyeong , Hong Young Sun , Sung Yeon-Ah , Lee Hyejin 
Published: September 2021

Abstract
Obesity is associated with cardiovascular diseases (CVD). However, body mass index (BMI) has a limited ability to measure abdominal obesity. We aimed to evaluate the associations between waist circumference (WC) and mortality or CVD incidence in a general Korean population. We analyzed a total of 204,068 adults older than 40 years of age who had undergone a national health examination in the Korean National Health Insurance Service Cohort. Hazard ratios for death and CVD incidence were calculated using Cox proportional hazards models after adjustment for age, smoking, alcohol consumption status, levels of physical activity, total cholesterol, hypertension, and diabetes mellitus status. In men, WC and overall mortality showed a reverse J-shaped association. For both men and women, WC was not associated with risk of cardiovascular mortality. Contrary to mortality trend, CVD incidence was positively associated with WC in both men and women, and risk of the CVD incidence was the lowest in subjects with a WC < 80 cm. WC exhibited a significant J-shaped association with overall mortality in men. The risk of incident CVD showed a positive association with central obesity, where the lowest risk was observed for subjects in the lowest WC group in a general Korean population.
Online Identification of Lithium-ion Battery Model Parameters with Initial Value Uncertainty and Measurement Noise
Authors: Du Xinghao , Meng Jinhao , Liu Kailong , Zhang Yingmin , Wang Shunli , Peng Jichang , Liu Tianqi 
Published: September 2021

Abstract
Online parameter identification is essential for the accuracy of the battery Equivalent Circuit Model (ECM). The traditional Recursive Least Squares (RLS) method is easily biased with the noise disturbances from sensors, which degrades the modeling accuracy in practice. Meanwhile, the Recursive Total Least Squares (RTLS) method can deal with the noise interferences, but the parameter slowly converges to the reference with initial value uncertainty. To alleviate the above issues, this paper proposes a co-estimation framework utilizing the advantages of RLS and RTLS for a higher parameter identification performance of the battery ECM. RLS converges quickly by updating the parameters along the gradient of the cost function. RTLS is applied to attenuate the noise effect once the parameters have converged. Both simulation and experimental results prove that the proposed method has good accuracy, fast convergence rate, and also robustness against noise corruption.
Fusion capacity after the implantation of interspinous fusion device - a biomechanical analysis and preliminary histological evaluation in pigs
Authors: Bao Li , Chen Mengmeng , Ji Xiang , Sun Qinchao , Tang Hai 
Published: September 2021

Abstract
BackgroudThe interspinous fusion device (ISFD) was widely used in the degenerative lumbar diseases. Previous clinical studies have demonstrated favorable efficacy. To further explore interspinous fusion capacity after ISFD implantation, we performed biomechanical analysis and preliminary histological evaluation.MethodsTwelves experimental pigs were selected and accepted a simulated operation of ISDF implantation. The animals were grouped six groups based on different procedures: sham surgery; unilateral laminotomy (UL); bilateral laminotomy (BL); ISFD + intact; ISFD + UL; ISFD + BL. The graft-bed site was filled with purified bone graft material without any autograft bone. After six months feeding, all experimental animals were sacrificed and the corresponding lumbar vertebrae was obtained. The samples were fixed on the spinal test system and the motion range of flexion-extension, lateral bending and rotation were tested through a multiaxial robotic system. The samples were prepared to cut using a hard tissue-slicing machine. Then, tissue sections stained with hematoxylin-eosin (HE) and toluidine blue were observed to evaluate the new-bone formation.ResultsLaminotomy increased lumbar mobility in comparison with intact model, especially for BL. The implantation of ISDF can reduce the range of motion in all motion directions. Well-defined bone tissue can be observed in the histological images after 6 months. In the histological part of toluidine blue staining, the area of newly formed bone accounted for 8.721%. The hyperchromatic osteoblasts cells and density bone tissue can be observed in HE staining slides.ConclusionsThe interspinous osteogenesis in histology confirmed the possibility of interspinous fusion. Moreover, the implantation of ISDF provide the necessary stabilization for promoting fusion.