bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidisciplinary Evaluation of Pacifier Removal on Oro-Dentofacial Structures: A Controlled Clinical Trial
Authors: Scudine Kelly Guedes de Oliveira , Freitas Camila Nobre de , Nascimento de Moraes Kizzy Silva Germano , Bommarito Silvana , Possobon Rosana de Fátima , Boni Rosana Cristina , Castelo Paula Midori 
Published: September 2021

It is well recognized that pacifier habit leads to occlusal and orofacial functional changes in children. However, the effects of the interruption of prolonged pacifier habit on the development of the dento-facial complex has not yet been fully characterized. Thus, the aim of this study was to investigate the influence of pacifier removal on aspects of oro-dentofacial morphology and function in preschool children. For that, a pacifier group (n = 28) and a control group (n = 32) of 4-year-old children with and without pacifier habit, respectively, were followed up by a group of dentists and speech therapists at baseline, 6 and 12 months after habit removal. Bite force and lip pressure were assessed using digital systems, and the evaluation of breathing and speech functions was performed using validated protocols, together with the measurements of dental casts and facial anthropometry. The Two-way mixed model ANOVA was used in data analysis. After 12 months, a decrease in malocclusion frequency was observed in pacifier group. Additionally, a change over time was observed in facial, intermolar and palate depth measurements, as well in bite and lip forces and speech function scores, increasing in both groups (p &lt; 0.01). The upper and lower intercanine widths and breathing scores differed between groups at baseline and changed over time reducing the differences. The presence of speech distortions was more frequent in the pacifier group at baseline and decreased over time (p &lt; 0.05). The interruption of pacifier habit improved the maxillary and mandibular intercanine widths, as well as the breathing and speech functions, overcoming the oro-dentofacial changes found.Trial Registration: This clinical trial was registered in the Brazilian Clinical Trials Registry (ReBEC; http://www.ensaiosclinicos.gov.br/), protocol no. RBR-728MJ2.
Utilizing Network Pharmacology to Explore the Possible Mechanism of Coptidis Rhizoma in Kawasaki Disease
Authors: Fan Xue , Guo Xin , Li Ying , Xu Mingguo 
Published: September 2021

Background: The purpose of the research is to identify the main active ingredients in Coptidis Rhizoma (CR) and explore the possible molecular mechanisms in the treatment of Kawasaki disease (KD).Materials and Methods: A total of 58 children with KD were randomly divided into a control group and a Berberine treatment group. The therapeutic indicators of the two groups before and after treatment were compared. Then, compounds and drug targets of CR from the TCMSP, SWISS, SEA, and the STITCH were collected, and targeted KD genes were retrieved from the DisGeNET, DrugBank, and GeneCards databases. The network pharmacology approach involved network construction, target prediction, and module analysis. GO and KEGG enrichment analysis were performed to investigate the possible pathways related to CR for KD treatments. Finally, protein expression was determined to verify the core targets using Western blotting in the cell experiment.Results: In total, nine compounds, 369 relative drug targets, and 624 KD target genes were collected in the above database. The network analysis revealed that 41 targets might be the therapeutic targets of CR on KD. GO and KEGG enrichment analysis revealed that the biological processes, namely, response to hormone, response to inorganic substance, and enzyme-linked receptor protein signaling pathway, and Pathways in cancer, Toll-like receptor signaling pathway, and Pancreatic cancer are the most significant. Protein expression of CASP3, PTGS2, and SRC was upregulated and AKT1 and ERK were downregulated.Conclusion: We provided useful resources to understand the molecular mechanism and the potential targets for novel therapy of KD.
CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
Authors: Zhong An-Ni , Yin Yi , Tang Bing-Jie , Chen Lei , Shen Hong-Wei , Tan Zhi-Ping , Li Wen-Qun , He Qun , Sun Bao , Zhu Yan , Xiao Jie , Jiang Zhi-Ping , Xu Ping 
Published: September 2021

Background: CircRNA has appeared as a critical molecular in the development of various cancers. However, the cellular function of circRNAs and exosomal circRNAs has not been well explored in Chronic myeloid leukemia (CML). Methods: Differentially expressed circRNAs were identified by a human circRNA microarray analysis. The expression of hsa_circ_0058493 in peripheral blood mononuclear cells (PBMCs) and exosomes was verified using quantitative real-time PCR. Short hairpin RNAs against hsa_circ_0058493 were constructed to silence the expression of circ_0058493. CCK8, flow cytometry and EdU assay were performed to investigate the biological functions of circ_0058493. Results: Hsa_circ_0058493 was significantly overexpressed in the PBMCs of CML patients and high level of circ_0058493 was associated with the poor clinical efficacy of imatinib. Silencing the expression of circ_0058493 significantly inhibited the development of imatinib-resistant CML cells. miR-548b-3p was overexpressed in circ_0058493-downregulated CML cells. Bioinformatic analysis revealed that circ_0058493 might exert its regulatory function acting as a “sponge” of miR-548b-3p. Moreover, hsa_circ_0058493 was significantly enriched in the exosomes derived from imatinib-resistant CML cells. Conclusion: Hsa_circ_0058493 in PBMCs could be a promising prognostic biomarker and might provide a therapeutic target for CML treatment.
Nimodipine Promotes Functional Recovery After Spinal Cord Injury in Rats
Authors: Guo Fangliang , Zheng Xiaolong , He Ziyu , Zhang Ruoying , Zhang Song , Wang Minghuan , Chen Hong , Wang Wei 
Published: September 2021

Spinal cord injury (SCI) is a devastating condition that results in severe motor, sensory, and autonomic dysfunction. The L-/T-type calcium channel blocker nimodipine (NMD) exerts a protective effect on neuronal injury; however, the protective effects of long-term administration of NMD in subjects with SCI remain unknown. Thus, the aim of this study was to evaluate the role of long-term treatment with NMD on a clinically relevant SCI model. Female rats with SCI induced by 25 mm contusion were subcutaneously injected with vehicle or 10 mg/kg NMD daily for six consecutive weeks. We monitored the motor score, hind limb grip strength, pain-related behaviors, and bladder function in this study to assess the efficacy of NMD in rats with SCI. Rats treated with NMD showed improvements in locomotion, pain-related behaviors, and spasticity-like symptoms, but not in open-field spontaneous activity, hind limb grip strength or bladder function. SCI lesion areas and perilesional neuronal numbers, gliosis and calcitonin gene-related peptide (CGRP+) fiber sprouting in the lumbar spinal cord and the expression of K+–Cl− cotransporter 2 (KCC2) on lumbar motor neurons were also observed to further explore the possible protective mechanisms of NMD. NMD-treated rats showed greater tissue preservation with reduced lesion areas and increased perilesional neuronal sparing. NMD-treated rats also showed improvements in gliosis, CGRP+ fiber sprouting in the lumbar spinal cord, and KCC2 expression in lumbar motor neurons. Together, these results indicate that long-term treatment with NMD improves functional recovery after SCI, which may provide a potential therapeutic strategy for the treatment of SCI.
Kanglaite Combined With Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor Therapy for Stage III/IV Non-Small Cell Lung Cancer: A PRISMA-Compliant Meta-Analysis
Authors: Kong Fanming , Wang Chaoran , Li Xiaojiang , Jia Yingjie 
Published: September 2021

Objective: Kanglaite(KLT), a type of Chinese medicine preparation, is considered as an adjuvant therapeutic option for malignant cancer treatment. This study aimed to systematically investigate the efficacy and safety of the combination of KLT and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) for the treatment of stage III/IV non-small cell lung cancer.Methods: Randomized controlled trials (RCTs) that compared KLT plus EGFR-TKI with EGFR-TKI alone for the treatment of stage III/IV non-small cell lung cancer were reviewed. Literature searches (up to July 10, 2021) were performed on PubMed, Web of Science, Cochrane Library, Embase, ClinicalTrials.gov, China National Knowledge Infrastructure (CNKI), Wanfang Database, and the Chinese Scientific Journal Database. Two researchers independently assessed the risk of bias with the tool of Cochrane Collaboration. RevMan 5.3.0 was used in the analysis of the included trial data.Results: 12 RCTs recruiting 1,046 patients with stage III/IV NSCLC were included. Results showed that compared with EGFR-TKI alone, KLT plus EGFR-TKI significantly increased the disease control rate (DCR) (odds ratio [OR]=3.26; 95% confidence interval [CI]:2.22–4.77; p &lt; 0.00001), the objective response rate (ORR) (OR=2.59; 95% CI:1.87–3.58; p &lt; 0.00001) and Karnofsky performance status (KPS) (OR = 2.76; 95% CI:1.73–4.39; p &lt; 0.00001). Furthermore, patient immunity was enhanced with KLT plus EGFR-TKI. The combined treatment increased the percentage of CD4 + T cells (weighted mean difference [WMD]=5.36; 95% CI:3.60–7.13; p &lt; 0.00001),the CD4+/CD8 + ratio (WMD = 0.18; 95% CI: 0.08–0.27; p = 0.004), and percentage of NK cells (WMD=4.84; 95% CI: 3.66–6.02; p &lt; 0.00001).With regard to drug toxicity, the occurrence rate of nausea and vomiting was significantly reduced by KLT plus EGFR-TKI (OR=0.37; 95% CI: 0.16–0.86; p = 0.02).Conclusion: KLT plus EGFR-TKI was effective in treating stage III/IV non-small cell lung cancer. Thus, its application in these patients is worth promoting. Additional double-blind, well-designed and multicenter RCTs are required to confirm the efficacy and safety of this treatment.
Epigenomic Regulators Elongator Complex Subunit 2 and Methyltransferase 1 Differentially Condition the Spaceflight Response in Arabidopsis
Authors: Paul Anna-Lisa , Haveman Natasha , Califar Brandon , Ferl Robert J. 
Published: September 2021

Background:Plants subjected to the novel environment of spaceflight show transcriptomic changes that resemble aspects of several terrestrial abiotic stress responses. Under investigation here is whether epigenetic modulations, similar to those that occur in terrestrial stress responses, have a functional role in spaceflight physiological adaptation. The Advanced Plant Experiment-04 – Epigenetic Expression experiment examined the role of cytosine methylation in spaceflight adaptation. The experiment was conducted onboard the International Space Station, and evaluated the spaceflight-altered, genome-wide methylation profiles of two methylation-regulating gene mutants [methyltransferase 1 (met1-7)and elongator complex subunit 2 (elp2-5)] along with a wild-type Col-0 control.Results:Theelp2-5plants suffered in their physiological adaptation to spaceflight in that their roots failed to extend away from the seed and the overall development of the plants was greatly impaired in space. Themet1-7plants suffered less, with their morphology affected by spaceflight in a manner similar to that of the Col-0 controls. The differentially expressed genes (DEGs) in spaceflight were dramatically different in theelp2-5andmet1-7plants compared to Col-0, indicating that the disruptions in these mutants resulted in a reprogramming of their spaceflight responses, especially inelp2-5. Many of the genes comprising the spaceflight transcriptome of each genotype were differentially methylated in spaceflight. In Col-0 the majority of the DEGs were representative of the now familiar spaceflight response, which includes genes associated with cell wall remodeling, pathogen responses and ROS signaling. However, the spaceflight transcriptomes ofmet1-7andelp2-5each presented patterns of DEGs that are almost completely different than Col-0, and to each other. Further, the DEGs of the mutant genotypes suggest a more severe spaceflight stress response in the mutants, particularly inelp2-5.Conclusion:Arabidopsis physiological adaptation to spaceflight results in differential DNA methylation in an organ-specific manner. Disruption of Met1 methyltransferase function does not dramatically affect spaceflight growth or morphology, yetmet1-7reprograms the spaceflight transcriptomic response in a unique manner. Disruption ofelp2-5results in poor development in spaceflight grown plants, together with a diminished, dramatically reprogrammed transcriptomic response.
Depression and Deliberate Self-Harm Among Rural Adolescents of Sichuan Province in Western China: A 2-Year Longitudinal Study
Authors: Lai Shimin , Su Chang , Song Shasha , Yan Mingxia , Tang Chengmeng , Zhang Qiang , Yin Fei , Liu Qiaolan 
Published: September 2021

Objective: To explore the change in the prevalence and association of depression and deliberate self-harm and their common and independent influencing factors among western Chinese rural adolescents.Methods: A total of 2,744 junior and senior high school students from two rural schools in Sichuan Province, China, participated in the baseline survey and were invited to participate in two follow-up surveys. The Center for Epidemiologic Studies-Depression Scale, a deliberate self-harm item, the Social Support Rating Scale, the Rosenberg Self-esteem Scale and the Connor-Davidson Resilience Scale were administered. A bivariate four-level logistic regression model was used for analysis.Results: The prevalence of depression and deliberate self-harm were 39.6 and 21.2%, respectively. Regular physical exercise, a good relationship with parents, high resilience, and high self-esteem were common protective factors for both depression and deliberate self-harm. Feeling disliked by teachers was a common risk factor for both. Being female, having a mother who emigrated as a migrant worker before the student was 3 years old, feeling disliked by classmates and having a poor family economic status were associated only with an increased risk of depression. Participants with medium social support were less likely to report deliberate self-harm than those with low or high support. Depression and deliberate self-harm were clustered at the class level.Conclusions: The comorbidity of depression and deliberate self-harm in rural adolescents should be given ample attention. Interventions should consider the class clustering of depression and deliberate self-harm and their common and unique influencing factors.
Mental Health Literacy in Zurich: A First Measurement Attempt Using the General HLS-EU-Q47
Authors: Schneider Michael , Jaks Rebecca , Nowak-Flück Daniela , Nicca Dunja , De Gani Saskia Maria 
Published: September 2021

Background: Mental health literacy (MHL) promises to be an important factor for public health by enabling people to take responsibility for their own mental health. To date, there is no measurement tool that allows the assessment of a comprehensive understanding of MHL as part of health literacy (HL). Nonetheless, the widely used Health Literacy Survey European Questionnaire 47 (HLS-EU-Q47) includes items assessing at least some MHL-aspects in the context of HL. The present study aimed at investigating how these MHL-aspects are related to HL, health behavior and health outcome and how they differ between sociodemographic groups.Methods: Data from the Health Literacy Survey Zurich 2018, collected by an adapted version of the HLS-EU-Q47, served to investigate these relationships.Results: MHL-aspects were related to HL, health behavior and health outcome. Nearly half of all respondents (45%; N = 904) showed low MHL levels, particularly those with higher age and higher financial deprivation.Conclusions: Relations of MHL-aspects with HL, health behavior, and health outcome indicate their potential importance for future interventions in public health, addressing mental health and MHL. A specific MHL tool is needed to comprehensively investigate these relations, which could be developed by extending the present measurement approach.
Thermodynamics of Reduced State of the Field
Authors: Cusumano Stefano , Rudnicki Łukasz 
Published: September 2021

Recent years have seen the flourishing of research devoted to quantum effects on mesoscopic and macroscopic scales. In this context, in Entropy 2019, 21, 705, a formalism aiming at describing macroscopic quantum fields, dubbed Reduced State of the Field (RSF), was envisaged. While, in the original work, a proper notion of entropy for macroscopic fields, together with their dynamical equations, was derived, here, we expand thermodynamic analysis of the RSF, discussing the notion of heat, solving dynamical equations in various regimes of interest, and showing the thermodynamic implications of these solutions.
Characterization of Phosphate Solubilizing Bacterial Endophytes and Plant Growth Promotion In Vitro and in Greenhouse
Authors: Mei Chuansheng , Chretien Robert L. , Amaradasa B. Sajeewa , He Yimeng , Turner Amy , Lowman Scott 
Published: September 2021

Phosphate is one of the most important nutrients for plant growth and development, and only 0.1% of the phosphate in soils is available to plants. Currently, the use of excess phosphate fertilizer has caused surface and ground water pollution and water eutrophication, resulting in algal blooms in lakes and oceans. Therefore, it is imperative to explore alternative ways to solve these problems for sustainable agricultural production and improvement of soil fertility, while protecting the environment. Microorganisms from the rhizosphere and within plants are able to solubilize insoluble soil phosphate, making it available to plants. Five high phosphate solubilizing bacteria from our bacterial endophyte library were chosen for this study and identified as Pantoea vagans IALR611, Pseudomonas psychrotolerans IALR632, Bacillus subtilis IALR1033, Bacillus safensis IALR1035 and Pantoea agglomerans IALR1325. All five bacteria significantly promoted tall fescue growth in vitro. Greenhouse experiments showed that IALR1325 significantly promoted pepper and tomato growth, and IALR632 was the best in promoting tomato growth. In addition, all these bacteria had extracellular acid phosphatase and phytase activities. One of the mechanisms for phosphate solubilization by bacteria is pH reduction caused by gluconic acid production. Our results indicate that P. agglomerans IALR1325 is a promising bacterium for future applications.
THE IDEA OF AN «IDEAL TEACHER» IN THE LIFE AND CREATIVITY OF THE ACADEMICIAN I. A. ZIAZIUN (1938-2014)
Authors: Lavrinenko Oleksandr 
Published: September 2021

The article deals with the scientific approaches of the outstanding Ukrainian educator, philosopher, organizer of pedagogical education I. A. Ziaziun (1938-2014) on the essence and content of the «ideal teacher» training; the academic work of the scientist in the domain of author’s ideas about the formation of the future teachers personality in the system of professional training, the development of leading universal values, humanistic principles of education and upbringing have been analyzed. Creative approaches to the formation of a high level of pedagogical activity-action – a dominant component of pedagogical skills have been elaborated.The scientific-theoretical approaches of I. A. Ziaziun to consideration the preparation specifics of an «ideal teacher» at pedagogical institutions of education, the formation of leading values, which should become a pledge of professional and pedagogical activities have been revealed.I. A. Ziaziun’s beliefs that an «ideal teacher» should be picked up from the school desk have been distinguished. «Ideal teacher» is first and foremost a person who provides a high level of communicative interaction, because all problems of learning and education are associated with ineffective communication, which is based on the lack of trust in the creative ability of the student’s personality and disrespect for his personality spontaneity motivated search for interest and meanings. It has been determined that the specificity of the «Ideal Teacher» personality as a subject of pedagogical action, according to I. A. Ziaziun consists of his personal qualities, experience, appearance, manners, behavior.It is proved that humanistic orientation of the teacher’s personality influence on the student’s behavior for the purpose of his subsequent change. Teacher’s actions are subordinated to the real interests of the individual. In this sense, the humanist orientation fully corresponds to the main tasks of personality-oriented pedagogy – to help a person in determining the relation to himself, other people, the world around him and his professional activities.As an unsurpassed aesthete and theorist of pedagogical skill, I. A. Ziaziun defended the most up-to-date value of teacher profession – the beauty of pedagogical action. The scientist convinced that the basis of human life is a feeling. Teachers, regardless of specialty, should be saturated with positive feelings of the environment of their students.
SCIENTIFIC SCHOOL OF ACADEMICIAN I. A. ZIAZIUN: AXIOLOGICAL ACTUALITIES OF DEVELOPMENT
Authors: Vovk Myroslava , Gryshchenko Julia 
Published: September 2021

The article presents the analysis of axiological foundations in the development of the scientific school of academician I. A. Ziaziun. It covers the conceptual idea of the scientific school of the scholar, based on ethical and aesthetic orientations of the pedagogical activity of a teacher, philosophical principles of the "pedagogy of good", value priorities of reforming the modern education and science. There are outlined basic directions of pedagogical studies of the scientific school: theoretical and methodological foundations in the development of pedagogical education, functioning of the system of postgraduate education, theory and practice of art education, professional training for teachers of art disciplines, personological aspect in the development of educational thought, foreign experience in training teachers, art studies in the course of defining the pedagogical potential of art, psychological and pedagogical aspects of in the development of children and young adults, retrospective of forming the national education, etc.Axiological actualities in the heritage of academician I. A. Ziaziun determine leading directions in the development of his scientific school of theory and practice of pedagogical skills, known in Ukraine and abroad. Perspective ideas of the academician, which contribute to the effectiveness of reformational changes in the educational and scientific development of the Ukrainian society, are being developed by scholars of the Institute of Pedagogical and Adult Education of the National Academy of Educational Sciences of Ukraine, where under the mentorship of I. A. Ziaziun formed a brilliant group of contemporary scholars, individuals with high methodological culture, fundamental training, and the humanistic style of scientific thinking. The heritage of the academician and ideas of his scientific school will be complemented by the generation of students, professors, teachers, scholars, and will always find the creative potential of research works, as well as the powerful resource for personal and professional growth.