bluuebunny/crossref_metadata_2025_split huggingface dataset:
Congenital Fibular Deficiency: Total Knee Arthroplasty with Extraarticular Deformity
Authors: Vedoya Santiago P. , Viale Germán , Gessara Alan , del Sel Hernán 
Published: April 2021

Case:
A total knee arthroplasty (TKA) was performed on a 35-year-old man with congenital fibular deficiency and a 20° varus and 28° antecurvatum tibial deformity of the left lower limb.

Conclusion:
One-stage TKA and correction of the extraarticular deformity by means of intraarticular bone resections and a standard soft tissue release were performed to restore the limb's mechanical axis. Patients with congenital fibular deficiency present a wide range of limb deformities because of bone deficiencies or treatment sequels, which might require a specific surgical technique and small-sized implants to obtain good results during a TKA.
Meralgia Paresthetica Caused by Melorheostosis Affecting the Ipsilateral Ilium
Authors: Miyoshi Hideaki , Nishisho Takehiko 
Published: April 2021

Case:
A 51-year-old woman presented with a 2-month history of pain in the right thigh. Radiography and computed tomography of the pelvis showed cortical hyperostosis of the right ilium. She was diagnosed with meralgia paresthetica (MP) caused by melorheostosis involving the right ilium. After 6 weeks of conservative management, an operation was performed for the persisting pain. At the 1-year follow-up, no findings of recurrence were observed clinically and radiographically.

Conclusion:
MP, caused by proximal irritation of the lateral femoral cutaneous nerve, can occur because of melorheostosis, which is a rare condition.
Comparison of soil biological properties and soil bacterial diversity in sugarcane, soybean, mung bean and peanut intercropping systems
Authors: Yang Shangdong , Xiao Jian , Huang Ziyue , Qin Renliu , He Weizhong , Liu Limin , Li Aomei , Tan Hong-Wei 
Published: April 2021

Abstract
Aims Sugarcane intercropping with soybean (Glycine max (Linn.) Merr.), mung bean (Vignaradiata (Linn.) Wilczek) and peanut (Arachishypogaea Linn.) as well as a sugarcane monoculture were conducted to study the impacts of intercropping on soil biological characteristics and bacterial diversity. Methods The soil samples were collected from twelve random sites and mixed well at the experimental farm of the Guangxi South Sub-tropical Agricultural Science Research Institute, Longzhou, China. Traditional analysis methods and modern high-throughput sequencing technology was used to compare and analyze the soil enzyme activity, microbial biomass, soil cultivable microorganisms and other biological traits and bacterial diversity.Results The results showed that soil cultivable microorganisms, the activities of soil enzymes and microbial biomass carbon, nitrogen, and phosphorus were all significantly improved by intercropping with soybean and mung bean. Additionally, soil bacterial diversity and richness in sugarcane fields were also significantly enhanced by intercropping with soybean and mung bean. In addition, soil bacterial community structures in sugarcane fields can be altered by intercropping with different legumes. Proteobacteria, a high-nutrient-tolerant bacterial assemblage, became the dominant bacteria in the sugarcane-soybean and sugarcane-mung bean intercropped soils. Twenty four, 28, 26 and 27 dominant soil bacterial genera were found after the sugarcane-soybean, sugarcane-mung bean, sugarcane-peanut and sugarcane monoculture treatments, respectively.Conclusions Sugarcane-mung bean intercropping suggested to be the most promising system for regaining and improving soil fertility and soil heath and facilitate agriculture intensification of sugarcane.
Flat-surface-assisted and self-regulated oxidation resistance of Cu(111)
Authors: Kim Su Jae , Kim Yong In , Lamichhane Bipin , Kim Young-Hoon , Lee Yousil , Cho Chae Ryong , Cheon Miyeon , Kim Jong , Jeong Hu Young , Ha Taewoo , Kim Jungdae , Lee Young Hee , Kim Seong-Gon , Kim Young-Min , Jeong Se-Young 
Published: April 2021

Abstract
The formation of an ultraflat surface on copper thin films is important for suppressing the oxidation process, but the formation of multi-atomic steps and terraces is a result of the basic atomic stacking process and difficult to avoid. Here, we develop an innovative strategy for fabricating copper thin films with a semi-permanently oxidation-resistant surface. Comprehensive atomic-resolution microscopy reveals that our thin film consists mostly of flat surfaces and occasional mono-atomic steps. Our first principles calculations show that a mono-atomic step edge is as impervious as a flat surface to oxygen consistent with our experimental observations. Furthermore, the first principles calculations exhibit self-regulated oxidation resistance at the top surface layer: when the oxygen coverage of fcc sites of the surface exceeds 50%, the adsorbed oxygens on the top layer suppresses further adsorption of additional O atoms and renders the ultraflat Cu surface to be more oxidation resistant.
Polycentric or monocentric, which kind of spatial structure is better for promoting the green economy? Evidence from Chinese urban agglomerations
Authors: Huang Yue , Liao Ruiwen 
Published: April 2021

Abstract
The green economy has gained worldwide attention, especially in the urban agglomerations where population and economic activities are highly concentrated. However, what kind of urban agglomeration spatial structure is more conducive to promoting the green economy? No clear conclusions have been made. Here, we study the impact of urban agglomeration spatial structure on the green economy, and also reveal how urban agglomeration spatial structure influences the three subsystems of green economy. We find that: (1) urban agglomeration spatial structural evolution is closely related to green economy, while in the research period, most urban agglomerations are not located in the optimal range of the spatial structure that drives the green economy. (2) Towards polycentric spatial structure is contributive to green economic growth, however, the excessively polycentric could not benefit green economy. (3) The evolution of urban agglomeration spatial structure exerts heterogenous impacts on the three subsystems when green economy is decomposed into economic subsystem, resources subsystem, and environmental subsystem. Towards polycentric is more conducive to the improvement of economic subsystem and resource subsystem, while, the tendency to monocentric drives the environmental subsystem development. (4) Lastly, our conclusions enlighten the urban agglomeration development planning and spatial mode for approaching a better performance in green economy.
Supplementation of green-synthesized nickel oxide nanoparticles enhances biohydrogen production of Klebsilla sp. using lignocellulosic hydrolysate and elucidation of the regulatory mechanism
Authors: Zhang Qin , Xu Siyuan , Li Yanbin , Ding Pengfei , Zhang Yonggui , Zhao Pei 
Published: April 2021

Abstract
Background

In recent years, adding nanoparticles to fermentative hydrogen production system has become an effective way to increase the biohydrogen yield, however, the application of green synthesized nanoparticles to hydrogen production system is rarely studied, even to the interpretation of the regulatory mechanism, there are few reports on the regulation of hydrogen production pathway and related gene expression by addition of nanoparticles. Thus, we herein reported the green synthesis of nickel oxide nanoparticles (NiO-NPs) from Eichhornia crassipes (Ec) extract for the first time, and evaluated the regulatory effect of these NPs on fermentative hydrogen production.
Results

Characterization of the Ec-NiO-NPs revealed their spherical shape, small diameter (9.1 ± 2.6 nm) and high purity. The maximum cumulative hydrogen production and hydrogen yield Y(H2/S) reached 4842.19 ± 23.43 mL/L and 101.45 ± 3.32 mL/gsubstrate, respectively, in the presence of 20 mg/L Ec-NiO-NP, which were 47.29% and 37.78% higher than the control without NPs addition. Evaluation of glucose and xylose utilization efficiency as well as key node metabolites further established the potential of Ec-NiO-NP to improve the reducing sugar utilization and metabolic flux distribution in hydrogen synthesis pathway. Furthermore, addition of 20 mg/L Ec-NiO-NP resulted in enhanced hydrogenase activity with a maximum increase of 623% comparing to the control, and led to changes in the gene expression of both hydrogenase and formate-hydrogen lyase, which play important roles in promoting hydrogen production at different stages of fermentation.
Conclusions

The results prove that supplementation with green-synthesized Ec-NiO-NP effectively improves fermentative hydrogen production and regulates key node metabolites alteration and functional gene expression. This study provides a cheap and eco-friendly method to enhance fermentative hydrogen production and new insights to reveal the regulatory mechanism underlying NP mediated increases in biohydrogen synthesis.
Investigations into the effects and related upstream/downstream regulatory mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells in vitro and in vivo
Authors: Zhang Xiaobei , Jin Meng , Liu Shiqi , Zang Mingde , Hu Lei , Du Tao , Zhang Baogui 
Published: April 2021

Abstract
Background

Cancer stem cells (CSCs) are proposed to be responsible for almost all malignant phenotypes (e.g. heterogeneity, uncontrolled growth, metastasis, recurrence, chemoresistance) of tumors. Long non-coding RNA WT1 antisense RNA (WT1-AS) has been found to be involved in the regulation of lung cancer cell stemness. However, the roles and molecular mechanisms of WT1-AS in the maintenance and development of gastric cancer stem cells (GSCs) have not been investigated.
Methods

mRNA and protein expression was measured by RT-qPCR and western blot. CCK8 and Soft agar colony formation assays were performed to assess cell viability and colony clone formation ability. Cell cycle and apoptosis were determined by flow cytometry analysis. Cell transwell and wound healing analyses were carried out to assess cell migration ability. In vitro angiogenesis and 3D spheroid cultures assays were also performed. Moreover, in vitro experiments were carried out to explore the function of WT1-AS on tumor growth, metastasis and cell stemness. The upstream transcription factors or downstream genes of WT1-AS were screened through Bioinformatics, dual-luciferase assays and RNA-sequencing (RNA-seq) technology.
Results

Our present study demonstrated that WT1-AS knockdown or wilms tumor 1 (WT1) overexpression improved GSC proliferative and migratory capacities, promoted GSC EMT, enhanced GSC stemness, inhibited GSC apoptosis, potentiated the resistance of GSCs to 5-FU and induced HUVEC angiogenesis in vitro. WT1-AS loss or WT1 increase facilitated the formation of in-vitro 3D GSC aggregates. WT1-AS ameliorated the malignant phenotypes of GSCs by down-regulating WT in vitro. Additionally, WT1-AS inhibited tumor growth and metastasis, and reduced tumor stemness in GSCs-derived xenografts (s.c., i.p., and i.v.) in vivo. Furthermore, XBP1 was identified as an upstream regulator of WT1-AS in GSCs. RNA-seq and RT-qPCR data suggested that PSPH, GSTO2, FYN, and PHGDH might be the downstream targets of WT1-AS in GSCs.
Conclusions

Our data demonstrated that WT1-AS weakened the stem-cell like behaviors and characteristics of GSCs in vitro and in vivo by down-regulating WT1. Also, some upstream regulators and downstream targets of WT1-AS were identified in GSCs. Investigations on the molecular mechanisms underlying the complex phenotypes of GSCs might contribute to the better management of headaches in cancers.
Technology-supported sitting balance therapy versus usual care in the chronic stage after stroke: A pilot randomized controlled trial
Authors: Thijs Liselot , Voets Eline , Wiskerke Evelien , Nauwelaerts Thomas , Arys Yves , Haspeslagh Harold , Kool Jan , Bischof Patrick , Bauer Christoph , Lemmens Robin , Baumgartner Daniel , Verheyden Geert 
Published: April 2021

Abstract
Insights in evidence on sitting balance and trunk rehabilitation have not resulted in specific device development. Hence, intensive one-by-one therapist-patient guidance is still required. We developed a novel rehabilitation prototype, specifically aimed at providing sitting balance therapy. In this study, we investigated if technology-supported sitting balance training was feasible and safe in chronic stroke patients and we evaluated whether clinical outcomes improved after a four-week programme when compared with usual care. Methods. In this parallel-group, assessor-blinded, randomized controlled pilot trial, we divided first event chronic stroke participants randomly into two groups. The experimental group received usual care plus additional therapy supported by rehabilitation technology consisting of 12 sessions of 50 minutes of therapy in four weeks. The control group received usual care only. We assessed all participants twice pre-intervention and once post-intervention. Feasibility and safety were descriptively analysed. Between-group analysis evaluated the differences in changes in motor and functional outcomes. Results. In total, 30 participants were recruited and 29 completed the trial (experimental group, n=14; control group, n=15). There were no between-group differences at baseline. Therapy was evaluated feasible by participants and therapist. There were no serious adverse events during sitting balance therapy. Changes in clinical outcomes from pre- to post-intervention demonstrated an increase in the experimental compared to the control group for trunk function; mean (standard deviation [SD]): 7.07 (1.69) versus 0.33 (2.35) points on trunk impairment scale (p<.000), maximum walking speed on 10-meter walk test (0.16 (0.16) m/s in the experimental group versus 0.06 (0.06) m/s in the control group; p=.003), and functional balance measured using Berg balance scale (median [interquartile range] 4.5 (5) points in the experimental group versus 0 (4) points in the control group (p=.014). Conclusions. Technology-supported sitting balance training in persons with chronic stroke is feasible and safe. A four-week, 12-hour programme on top of usual care suggests beneficial effects for trunk function, maximum gait speed and functional balance.Trial Registration: ClinicalTrials.gov identifier: NCT04467554, https://clinicaltrials.gov/ct2/show/NCT04467554, date of Registration: 13 July 2020.
Experiment Regarding Magnetic Fields with Gravity
Authors: LEE JONG-HOON 
Published: April 2021

Abstract
We observed the Earth's gravity to produce electricity in the magnetic sea. The gravity generator (GG) generated currents and voltages on the mesoscopic scale when we connected it in the vacuum. Gravity interacts to generate electricity in the Earth’s direction or the opposite direction by the repulsive magnetic force. The ground-based device simulates the potential energy (voltages) between gravity and magnetic seas. The GG increased simulations of gravity quantum effects using different physical set-ups. A trapped gravity was set to behave as free relativistic quantum particles or fluids, which made it possible to measure the particle position as a function of time and study the magnetic sea for different initial superpositions of positive- negative-gravity spinor state. It might explain the relativistic quantum equations, antigravity, and the heating mechanism of the Sun.
Polymorphic estrogen receptor binding site causes CD2-dependent sex bias in the susceptibility to autoimmune diseases
Authors: Lahore Gonzalo Fernandez , Förster Michael , Johannesson Martina , Sabatier Pierre , Lönnblom Erik , Aoun Mike , He Yibo , Nandakumar Kutty , Zubarev Roman , Holmdahl Rikard 
Published: April 2021

Abstract
Complex autoimmune diseases are sexually dimorphic. An interplay between predisposing genetics and sex-related factors likely determines the sex discrepancy in the immune response, but conclusive evidence is lacking regarding the underlying molecular mechanisms. Using forward genetics, we positionally identified a polymorphic estrogen receptor binding site that regulates CD2 expression, leading to female-specific differences in mouse models of T cell-dependent autoimmunity. Female mice with reduced CD2 levels displayed diminished expansion of autoreactive T cells. Mechanistically, CD2 affected T cell activation by inhibiting LAG-3 expression. Our findings explain the sexual dimorphism in human autoimmunity, as CD2 associated with rheumatoid arthritis and its regulation through 17-β-estradiol was conserved in human T cells. Hormonal regulation of CD2 has implications for CD2-targeted therapy. Indeed, anti-CD2 treatment was more potent in female mice. In conclusion, our results demonstrate the relevance of sex-genotype interactions and provide strong evidence for CD2 as a sex-sensitive predisposing factor in autoimmunity.
Addition of Al(OH)3 vs. AlO(OH) nanoparticles on the optical, thermo-mechanical and heat/oxygen transmission properties of microfibrillated cellulose films
Authors: Kolar Tjaša , Mušič Branka , Korošec Romana Cerc , Kokol Vanja 
Published: April 2021

Abstract
Differently structured aluminum (tri/mono) hydroxide (Al(OH)3 / AlO(OH)) nanoparticles were prepared and used as thermal-management additives to microfibrillated cellulose (MFC), cast-dried in thin-layer films. Both particles increased the thermal stability of the MFC film, yielding 20–23% residue at 600 °C, and up to 57% lowered enthalpy (to 5.5–7.5 kJ/g) at 0.15 wt% of loading, while transforming to Al2O3. However, the film containing 40 nm large Al(OH)3 particles decomposed in a one-step process, and released up to 20 % more energy between 300–400°C as compared to the films prepared from smaller (21 nm) and meta-stable AlO(OH), which decomposed gradually with an exothermic peak shifted to 480 °C. The latter resulted in a highly flexible, optically transparent (95%), and mechanically stronger (5.7 GPa) film with a much lower specific heat capacity (0.31 − 0.28 J/gK compared to 0.68–0.89 J/gK for MFC-Al(OH)3 and 0.87–1.26 for MFC films), which render it as an effective heat-dissipating material to be used in flexible opto-electronics. Low oxygen permeability (2192.8 cm3/m2day) and a hydrophobic surface (>60°) rendered such a film also useful in ecologically-benign and thermosensitive packaging.
NEW DAD STUDY (NEST): A mixed-methods feasibility study of an intervention to improve men’s mental health and wellbeing during their transition to fatherhood
Authors: Baldwin Sharin , Malone Mary , Murrells Trevor , Sandall Jane , Bick Debra 
Published: April 2021

Abstract
Background: Many health visiting services in England use the Promotional Guide system with mothers and fathers, an intervention to support their transition to parenthood, but there is little known about its use and effectiveness, especially with fathers. The aim of this study was to test the feasibility and acceptability of the Promotional Guide system with first-time fathers and pilot potential outcome measures to assess their mental health and wellbeing. Methods: A mixed methods prospective observational cohort study. Expectant first-time fathers were recruited from four London (UK) local authority boroughs. Data were collected through online pre and post intervention questionnaires, and semi-structured telephone interviews. Quantitative data were analysed using descriptive statistics and qualitative data were analysed using framework analysis. Results: Eighty-six fathers were interested in participating; 7 men did not meet inclusion criteria and 79 were invited to complete the baseline questionnaire. Questionnaires completed by 45 men at both timepoints were included in the final analysis. Ten of these men were also interviewed. Six major categories were identified: 1) Experience of health visitor contact, 2) Experience of Promotional Guides, 3) Experience of perinatal health services, 4) Experience of fatherhood, 5) Fathers’ mental health and wellbeing, and 6) Experience of the research process. While antenatal and postnatal outcomes were collected from 45 first-time fathers, none had received the intervention in its entirety. This study identified major gaps in the implementation of the Promotional Guide system with fathers. Conclusion: This study highlighted first-time fathers’ mental health and wellbeing needs during their transition to fatherhood, and how they wanted to be supported. It also identified barriers to implementation of the Promotional Guide system by health visitors, which need to be addressed prior to any future research into this intervention. These findings have a number of implications for researchers, health professionals, health service managers, commissioners, policy makers and parents.
Title Knowledge, attitude and use of evidence based practice (EBP) among Registered Nurse-Midwives practicing in Central Hospitals in Malawi: A Cross-Sectional Survey
Authors: Kaseka Paul Uchizi , Mbakaya Balwani Chingatichifwe 
Published: April 2021

Abstract
Background: Even though EBP is being considered as a critical element in improving the quality of health services and achieving excellence in patient care, there is currently little knowledge of how EBP relates to nursing and midwifery in Malawi. This paper is a report of a study describing EBP knowledge, attitudes and use of registered nurses-midwives practicing in central hospitals in Malawi. Methods: The descriptive, cross-sectional research design was conducted with a convenient sample of 183 nurse-midwives (response rate of 86%). The study used a questionnaire to collect data. Data were analysed using both descriptive and inferential statistics in the Statistical Product and Service Solutions version 23. Descriptive statistics were calculated to summarize overall knowledge levels, attitudes and use of nurse-midwives as percentages based on their scores from the assessment scale (1 to 7 Likert scale) in the EBP questionnaire. Non-parametric Mann-Whitney and Kruskal-Wallis tests were carried out to compare evidence-based practice scores based on demographics. Pearson’s correlation (r) and stepwise regression analysis were further performed to analyse the relationship between the knowledge, attitude and use of nurse-midwives on the overall EBP of nurse-midwives. Results: The average scores (mean±SD) of evidence-based practice amongst nurse midwives were 78.7±19.6 for attitude, 70.6±15.1 for knowledge levels, 57.8±23.for use, and 68.9±14.2 for the overall EBP. Higher educational qualification was associated with higher scores in knowledge levels (P=0.02). Research experience was associated with higher scores in nursing use (P=0.005), and higher overall evidence based practice were associated with both research experience (P=0.035) and educational qualification (P=0.004). Nurse-midwives attitude was affected by clinical experience (P=0.006) and the hospital where nurse-midwives worked (P=0.016). There was no significant difference in the EBP scores of nurse-midwives based on gender and/or their administrative roles in their respective central hospitals Conclusion: It is important to develop the knowledge/skills of nurse midwives in order to enhance evidence based practice amongst nurse midwives in Malawian hospitals. The results can be used by nurse managers, nurse educators, Ministry of Health policy makers, Nurses and Midwives Council of Malawi and donors to enhance implementation of EBP.