bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiac Derived CD51-Positive Mesenchymal Stem Cells Enhance the Cardiac Repair Through SCF-Mediated Angiogenesis in Mice With Myocardial Infarction
Authors: Xie Dong Mei , Chen Yang , Liao Yan , Lin Wanwen , Dai Gang , Lu Di Han , Zhu Shuanghua , Yang Ke , Wu Bingyuan , Chen Zhihong , Peng Chaoquan , Jiang Mei Hua 
Published: April 2021

Objective:Many tissues contained resident mesenchymal stromal/stem cells (MSCs) that facilitated tissue hemostasis and repair. However, there is no typical marker to identify the resident cardiac MSCs. We aimed to determine if CD51 could be an optimal marker of cardiac MSCs and assess their therapeutic potential for mice with acute myocardial infarction (AMI).Methods:Cardiac-derived CD51+CD31–CD45–Ter119–cells (named CD51+cMSCs) were isolated from C57BL/6 mice(7-day-old) by flow cytometry. The CD51+cMSCs were characterized by proliferation capacity, multi-differentiation potential, and expression of typical MSC-related markers. Adult C57BL/6 mice (12-week-old) were utilized for an AMI model via permanently ligating the left anterior descending coronary artery. The therapeutic efficacy of CD51+cMSCs was estimated by echocardiography and pathological staining. To determine the underlying mechanism, lentiviruses were utilized to knock down gene (stem cell factor [SCF]) expression of CD51+cMSCs.Results:In this study, CD51 was expressed in the entire layers of the cardiac wall in mice, including endocardium, epicardium, and myocardium, and its expression was decreased with age. Importantly, the CD51+cMSCs possessed potent self-renewal potential and multi-lineage differentiation capacityin vitroand also expressed typical MSC-related surface proteins. Furthermore, CD51+cMSC transplantation significantly improved cardiac function and attenuated cardiac fibrosis through pro-angiogenesis activity after myocardial infarction in mice. Moreover, SCF secreted by CD51+cMSCs played an important role in angiogenesis bothin vivoandin vitro.Conclusions:Collectively, CD51 is a novel marker of cardiac resident MSCs, and CD51+cMSC therapy enhances cardiac repair at least partly through SCF-mediated angiogenesis.
Tumor Mutation Burden and Immune Invasion Characteristics in Triple Negative Breast Cancer: Genome High-Throughput Data Analysis
Authors: Gao Chundi , Li Huayao , Liu Cun , Xu Xiaowei , Zhuang Jing , Zhou Chao , Liu Lijuan , Feng Fubin , Sun Changgang 
Published: April 2021

In recent years, the emergence of immunotherapy has provided a new perspective for the treatment and management of triple-negative breast cancer (TNBC). However, the relationship between tumor mutation burden (TMB) and immune infiltration and the prognosis of TNBC remains unclear. In this study, to explore the immunogenicity of TNBC, we divided patients with TNBC into high and low TMB groups based on the somatic mutation data of TNBC in The Cancer Genome Atlas (TCGA), and screened out genes with mutation rate ≥10. Then, Kaplan-Meier survival analysis revealed that the 5-year survival rate of the high TMB group was much higher than that of the low TMB group and the two groups also showed differences in immune cell infiltration. Further exploration found that the FAT3 gene, which displays significant difference and a higher mutation rate between the two groups, is not only significantly related to the prognosis of TNBC patients but also exhibits difference in immune cell infiltration between the wild group and the mutant group of the FAT3 gene. The results of gene set enrichment analysis and drug sensitivity analysis further support the importance of the FAT3 gene in TNBC. This study reveals the characteristics of TMB and immune cell infiltration in triple-negative breast cancer and their relationship with prognosis, to provide new biomarkers and potential treatment options for the future treatment of TNBC. The FAT3 gene, as a risk predictor gene of TNBC, is considered a potential biological target and may provide new insight for the treatment of TNBC.
IgG4-Related Disease With Tuberculosis: A Case Report and Retrospective Review of Patients in a Single Center
Authors: Qing Pingying , Lu Chenyang , Liu Zhihui , Wen Xiuzhen , Chen Bo , Lin Zhiguo , Ma Yingbing , Zhao Yi , Liu Yi , Tan Chunyu 
Published: April 2021

BackgroundIgG4-related disease (IgG4-RD) is a recently recognized systemic fibro-inflammatory disease of unknown cause involving many organs including pancreas, salivary glands, and lymph nodes. Chronic tuberculosis (TB) infection has been reported in IgG4-RD, but the prevalence of TB infection has not been evaluated in IgG4-RD.MethodsCharacterization of a patient with IgG4-RD by physical examination, laboratory tests, magnetic resonance imaging (MRI) and histological examination. TB infection was evaluated by medical history, radiological examinations, sputum examinations, tubercular skin test (TST) and interferon gamma (IFN-γ) release assay test (IGRA). Medical records of IgG4-RD patients were reviewed in our institute from February 2015 to September 2020 to explore the prevalence of TB infection in IgG4-RD.ResultsWe described a 40-year-old Chinese man presented with headache and diplopia. Physical examination revealed bitemporal hemianopsia and limited abduction of both eyes. MRI revealed uniformly enhancing mass overlying clivus with dural tail sign. Laboratory data revealed elevation of IgG4 (1.9g/L), and TB-IGRA demonstrated significantly elevated IFN-γ (414.21 pg/ml). The clivus lesion was subtotally removed and IgG4 was strongly positive on immunohistochemical staining. The diagnosis of IgG4-RD was established, and the patient received treatment of corticosteroids, methotrexate, and cyclophosphamide with isoniazid prophylaxis. Consequently, the mass shrank remarkably within 3 months. A similar concurrence of TB disease or latent TB infection (LTBI) and IgG4-RD was present in 17/47 (36.2%) patients in our institute.ConclusionHigh frequency of TB/LTBI presented in patients with IgG4-RD. Patients with IgG4-RD and LTBI should be closely monitored for resurgence of TB. Whether TB represents a risk for IgG4-RD should be further investigated in prospective cohort.
Association of Olfactory Impairment and Postoperative Cognitive Dysfunction in Elderly Patients
Authors: Lan Yang , You Zhi-jian , Du Ruiming , Chen Le-si , Wu Jia-xuan 
Published: April 2021

ObjectiveTo investigate the impact of anesthesia on the change of olfactory function and cognitive function in elderly patients who undergo abdominal surgery.MethodsA total of 30 elderly patients who underwent abdominal surgery were recruited as the research subjects. The Connecticut Chemosensory Clinical Research Center (CCCRC) olfactory test was used to test the olfactory function and the Mini-mental State Examination (MMSE), Hopkins Verbal Learning Test – Revised (HVLT-R), Trail Making Test (TMT), Stroop Color Word Test (SCWT), Digit-Symbol Coding Test (DSCT), and Verbal Fluency Test (VFT) were used to assess their cognitive function before general anesthesia, and on the 3rd and 7th day post-anesthesia. The serum level of IL-1β, IL-6, and TNF-α were measured before anesthesia and at 0, 12, and 24 h post-anesthesia. In total, 30 healthy volunteers who did not undergo anesthesia were used as the control group. The test results of all subjects were recorded and their correlations were analyzed.ResultsOn the 3rd and 7th day post-anesthesia, the olfactory recognition threshold of patients in the surgical group was lower than that of control group with significant difference (P &lt; 0.05). On the 3rd and 7th postoperative day, the patient’s short-term memory and delayed memory, attention and processing speed were decreased (P &lt; 0.05). On the 7th day post-anesthesia, delayed memory and processing ability were still decreased (P &lt; 0.05). In the surgical group, Spearman correlation analysis showed that the difference of olfactory recognition score on the 3rd and 7th day post-anesthesia was positively correlated with short-term memory and delayed memory of cognitive function. Compared with pre-anesthesia, the serum levels of IL-1β, IL-6, and TNF-α in the surgical group were significantly increased at each time point after anesthesia.ConclusionAbdominal surgery with general anesthesia in elderly patients may increase the level of serum inflammatory factors, induce olfactory impairment, particularly the decline of olfactory identification threshold and cause cognitive dysfunction with declined short-term memory, delayed memory and attention. There was a positive correlation between olfactory impairment and cognitive dysfunction after general anesthesia. Therefore, olfactory impairment could be an early indicator to guide early intervention for postoperative cognitive dysfunction.
Influence of Cochlear Implantation on Vestibular Function in Children With an Enlarged Vestibular Aqueduct
Authors: Wang Ruijie , Zhang Daogong , Luo Jianfen , Chao Xiuhua , Xu Jiliang , Liu Xianfeng , Fan Zhaomin , Wang Haibo , Xu Lei 
Published: April 2021

Background: Cochlear implantation (CI) is becoming increasingly used in the rehabilitation of hearing-impaired patients. Children with an enlarged vestibular aqueduct (EVA) need CI for severe or profound hearing loss, with excellent outcomes in hearing rehabilitation. However, vestibular function influenced by CI in children with EVA has not been clarified. We compared the characteristics of vestibular function in implanted children with EVA and those with a normal cochlea.Methods: In this retrospective case-control study, 16 children with large vestibular aqueduct syndrome (LVAS) and 16 children with a normal cochlea were recruited as the Study and Control Group, respectively. All children (mean age, 10.3 ± 4.4 years) had bilateral profound sensorineural hearing loss (SNHL) and normal pre-operative vestibular functions and underwent unilateral CI. Otolith and canal functions were assessed before CI and 12 months thereafter. Cervical vestibular-evoked myogenic potential (cVEMP), ocular vestibular-evoked myogenic potential (oVEMP), and video head impulse test (vHIT) were evaluated.Results: Full insertion of the electrode array was achieved in all the cases. Preoperatively, no significant differences in parameters in cVEMP between the Study and Control Group were revealed (p &gt; 0.05). In pre-operative oVEMP, shorter N1 latencies (p = 0.012), shorter P1 latencies (p = 0.01), and higher amplitudes (p = 0.001) were found in the Study than in the Control Group. The Study Group had shorter P1 latency in cVEMP (p = 0.033), and had lower amplitude in oVEMP after implantation (p = 0.03). Statistically significant differences were not found in VOR gains of all three semicircular canals before and after surgery (p &gt; 0.05). VEMP results revealed that the Control Group had significantly lower deterioration rates after CI (p &lt; 0.05). The surgical approach and electrode array had no statistically significant influence on the VEMP results (p &gt; 0.05).Conclusion: oVEMP parameters differed between children with EVA and children with a normal cochlea before surgery. Systematic evaluations before and after CI showed that otolith function was affected, but all three semicircular canals functions were essentially undamaged after implantation. In contrast to subjects with a normal cochlea, children with EVA are more likely to preserve their saccular and utricular functions after CI surgery. Possible mechanisms include less pressure-related damage, a reduced effect in terms of the air-bone gap (ABG), or more sensitivity to acoustic stimulation.
125I Seed Promotes Apoptosis in Non-small Lung Cancer Cells via the p38 MAPK-MDM2-p53 Signaling Pathway
Authors: Zhang Tao , Mo ZhiQiang , Duan Guangfeng , Tang Rijie , Zhang Fujun , Lu Mingjian 
Published: April 2021

Purpose:125I seeds were effective in the treatment of non-small cell lung cancer in previous research. However, the exact signaling pathway-mediated apoptosis mechanism is still unclear. The present study analyzed the effects and potential mechanisms of 125I seed on the growth and migration of A549 cells.Methods: Lung cancer A549 cells were irradiated with 125I seed for various times. MTT, invasion assay, and flow cytometry were used to detect the proliferation, invasion, and apoptosis of treated cells, respectively. A Nimblegen genome-wide expression profile chip was used to evaluate gene expression changes in 125I seed-treated A549 cells. Validation studies were performed using phosphorylated protein chip technology, Western blot, nude mouse tumor xenograft assay, and immunohistochemical experiments. All statistical analyses were performed using unpaired Student's t tests and Kruskal-Wallis test.Results: Irradiation with 125I seed inhibited A549 cell proliferation and invasion and induced apoptosis (primarily early apoptosis). Irradiation with 125I seed also caused the downregulation of p38MAPK, degradation of mouse double-minute 2 homolog (MDM2), and higher expression of p53, which eventually resulted in non-small cell lung cancer cell apoptosis.Conclusion:125I seed irradiation activated the p38MAPK/MDM2/p53 signaling pathway and promoted non-small cell lung cancer cell apoptosis. Future clinical studies targeting this signal may provide a new potential therapeutic approach for non-small cell lung cancer.
The Clinical Accuracy and Risk Stratification in End of Therapy 18F-FDG PET/CT in Burkitt Lymphoma
Authors: Mo Yi Wen , Xiao Zi Zheng , Wei Yuan , Li Xin Ling , Zhang Xu , Fan Wei 
Published: April 2021

PurposeBurkitt lymphoma (BL) is an invasive lymphoma subtype with FDG avid at 18F-FDG PET/CT, but there is currently no validated criterion in treatment evaluation and prognosis prediction. The aim of this study was to analyze the clinical accuracy of 18F-FDG PET/CT in Burkitt lymphoma in end of therapy PET/CT (EOT-PET) to assess the treatment response in BL and conduct a survival analysis with different Deauville 5-point score (DS) cutoff values.Materials and MethodsA total of 189 patients were retrospectively included: 97 underwent baseline PET/CT and all underwent EOT-PET. Survival curves were plotted according to the Kaplan-Meier method. Different DS cutoff values in EOT-PET were evaluated for risk stratification in Burkitt lymphoma.ResultsThe median progression free survival (PFS) and overall survival (OS) were 52 and 53 months, respectively. Applying the conventional DS 4 to 5, there was significant difference in outcome between EOT-PET negative and positive patients. However, the positive predictive value (PPV) (28.3% for PFS and 26.4% for OS) is low despite a high negative predictive value (NPV) (94.1% for OS and 94.9% for OS). When we moved the cutoff point to DS 5, the PPV was improved evidently (88.2% for PFS and 82.3% for OS) with the satisfactory NPV simultaneously (95.3% for PFS and 95.9% for OS).ConclusionsEOT-PET results using DS significantly related with PFS and OS. DS of 5 may be a better cutoff point at the end of treatment to determine whether patients have a significant risk of recurrence or progress.
Clinicopathological Characteristics and Prognostic Prediction in Pseudomyxoma Peritonei Originating From Mucinous Ovarian Cancer
Authors: Fu Fengxian , Ma Xulan , Lu Yiyan , Xu Hongbin , Ma Ruiqing 
Published: April 2021

ObjectiveTo describe the clinicopathological characteristics of mucinous ovarian cancer (MOC)-derived pseudomyxoma peritonei (PMP) and identify prognostic factors for survival.MethodsMedical records from patients with MOC-derived PMP who attended the Aerospace Center Hospital, Beijing, China between January 2009, and December 2019 were retrospectively reviewed. Survival analysis was performed with the Kaplan-Meier method, the log-rank test, and a Cox proportional hazards model.ResultsCytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) for PMP originating from MOC were performed on 22 patients, who had a median age of 52 years at the time of surgery. At the last follow-up in June 2020, 9 (41%) patients were still alive. Median OS was 12 months (range, 1 to 102 months), and the 2-, 3-, and 5-year survival rates were 23, 9, and 5%, respectively.ConclusionHistopathologic subtype and PCI may be applied as predictors of prognosis in patients with MOC-derived PMP. Patients with high-grade disease could benefit from completeness of cytoreduction (CCR) 0/1.
Hesitation and Refusal Factors in Individuals' Decision-Making Processes Regarding a Coronavirus Disease 2019 Vaccination
Authors: Cerda Arcadio A. , García Leidy Y. 
Published: April 2021

Introduction:Considering the global prevalence of coronavirus disease 2019 (COVID-19), a vaccine is being developed to control the disease as a complementary solution to hygiene measures—and better, in social terms, than social distancing. Given that a vaccine will eventually be produced, information will be needed to support a potential campaign to promote vaccination.Objective:The aim of this study was to determine the variables affecting the likelihood of refusal and indecision toward a vaccine against COVID-19 and to determine the acceptance of the vaccine for different scenarios of effectiveness and side effects.Materials and Methods:A multinomial logistic regression method based on the Health Belief Model was used to estimate the current methodology, using data obtained by an online anonymous survey of 370 respondents in Chile.Results:The results indicate that 49% of respondents were willing to be vaccinated, with 28% undecided or 77% of individuals who would potentially be willing to be inoculated. The main variables that explained the probability of rejection or indecision were associated with the severity of COVID-19, such as, the side effects and effectiveness of the vaccine; perceived benefits, including immunity, decreased fear of contagion, and the protection of oneself and the environment; action signals, such as, responses from ones' family and the government, available information, and specialists' recommendations; and susceptibility, including the contagion rate per 1,000 inhabitants and relatives with COVID-19, among others. Our analysis of hypothetical vaccine scenarios revealed that individuals preferred less risky vaccines in terms of fewer side effects, rather than effectiveness. Additionally, the variables that explained the indecision toward or rejection of a potential COVID-19 vaccine could be used in designing public health policies.Conclusions:We discovered that it is necessary to formulate specific, differentiated vaccination-promotion strategies for the anti-vaccine and undecided groups based on the factors that explain the probability of individuals refusing or expressing hesitation toward vaccination.
PERBEDAAN PENGETAHUAN IBU HAMIL TRIMESTER I SETELAH DIBERIKAN SELF MANAGEMENT EDUCATION MENGHADAPI EMESIS GRAVIDARUM DI BPM WILAYAH KERJA PUSKESMAS IV DENPASAR SELATAN
Authors: Witari Ni Nyoman Deni , Dewianti Ni Made 
Published: April 2021

Mual muntah adalah salah satu gejala paling awal, paling umum dan paling menyebabkan stress pada awal kehamilan. Terapi nonfarmakologi merupakan jenis terapi komplementer yang dapat digunakan sebagai intervensi untuk mengatasi mual. Self management education emesis gravidarum mengadopsi konsep dari Corbin dan Straus dalam Kate dan Halsted (2003) tersebut, yang dituangkan dalam bentuk modul yang bertujuan agar ibu hamil dapat mengedukasi diri sendiri sehingga mampu untuk mengatur hidup sendiri, mengatur tujuan, dan menyediakan penguat untuk diri sendiri dalam menangani mual muntah yang dialami.Tujuan penelitian ini adalah untuk mengetahui perbedaan pengetahuan setelah self management education menghadapi emesis gravidarum. Penelitian ini penelitian analitik dengan desain penelitian pre eksperimen (quasi experiment design) dengan rancangan one group pre test – post test design. Penelitian ini akan dilakukan di BPM Wilayah Kerja Puskesmas IV Denpasar Selatan . Populasi penelitian ini ibu hamil yang datang ke BPM wilayah kerja puskesmas IV Denpasar Selatan. Sampel penelitian ini ibu hamil yang mengalami mual muntah pada trimester I yang memenuhi kriteria inklusi dimana tehnik sampling pada penelitian ini acidental sampling . Analisa Bivariat akan dilakukan uji t Test untuk menguji perbedaan dua distribusi pre dan post test sebelum diberikan self management education dan setelah pemberian self management education menghadapi emesis gravidarum. rata-rata pengetahuan sebelum diberikan modul self management adalah 59,1 , nilai standar deviasi 10,856, nilai minimum dan maksimum 33-87. Nilai rata-rata pengetahuan setelah diberikan modul self management adalah 73,6 , nilai standar deviasi 13,874 dengan nilai minimum dan maksimum 47-100

Kata Kunci : Pengetahuan, Ibu hamil Trimester I, Mual Muntah
Wybrane zagadnienia zmian legislacyjnych w czasie pandemii
Authors: Wrześniewska-Wal Iwona , Augustynowicz Anna , Hajdukiewicz Dariusz , Janiszewska Lidia , Waszkiewicz Michał , Zgliczyński Wojciech 
Published: April 2021

The aim of the article was to present selected problems of health policy in the context of legislative changes in health care on the example of activities related to the SARS-CoV-2 virus epidemic, as well as to show the scope and pace of changes and their assessment. The first group of issues covers the analysis of the provisions introduced regarding organizational changes in entities providing medical activities, which, from the beginning of the pandemic, changed the scope of inpatient care and developed teleporting activities. The second group consists of changes that overlapped with regulations counteracting SARS-CoV-2 virus infections in the scope of GDPR and medical documentation. The third is an analysis of legal provisions regulating in detail the rights and obligations of medical personnel and patients, which on the one hand modify the legal liability of persons providing services, and on the other, introduce restrictions on access to them.
Latihan "Salam Trendi" terhadap Kekuatan Otot dan Kecepatan Berjalan Lansia Paska Stroke di Kota Depok
Authors: Ratnawati Emmelia 
Published: April 2021

Stroke in the elderly resulted in limited autonomy. Exercise Salam Trendy consists of deep breathing exercises, stretching, and range of motion is one exercise to reduce limitations. Exercises done at home and individual 2x / week for 4 weeks. This study aimed to identify the effects of exercise "Salam Trendy" against muscle strength and walking speed of elderly post-stroke in Depok. Quasi-Experimental research design using pre and post test design. Total sample of 44 respondents consisting of the treatment group and the control group were selected with consecutive sampling technique. Evaluation studies conducted on the first and fourth week. The results showed that the arm muscle strength (p = 0.042) and legs (p = 0.005); walking speed (p = 0.002) increased significantly after being given a workout. This means that the exercise “Salam Trendy “ affect the increase in muscle strength and walking speed. The study recommends the need for this exercise as one of the interventions of nurse elderly post-stroke in the community.
Keyword: Stroke; the elderly; exercise Salam Trendy; muscle strength, walking speed.