bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative factors associated with intraoperative maximum arterial pressures in patients with pheochromocytoma and paraganglioma: a single-center retrospective study
Authors: Ohsugi Haruyuki , Takizawa Nae , Matsuda Tadashi , Kinoshita Hidefumi 
Published: September 2021

Abstract
Surgery for pheochromocytoma and paraganglioma (PPGL) can lead to life-threatening complications, such as intraoperative hypertensive crises, even when adequate doses of preoperative α-receptor blockades are administered. The aim of this study was to identify preoperative factors associated with maximum arterial pressure (AP) during surgery in patients with PPGL. We retrospectively reviewed the cases of 61 PPGL patients who underwent surgical resection in our hospital between 2006 and 2020. The primary outcome was intraoperative maximum AP as a single index for continuous variables. The normal distribution of the results was confirmed by the Kolmogorov–Smirnov test. Simple and multiple linear regression model were used for statistical analysis. The median maximum systolic AP during surgery was 165 mmHg (interquartile range: 150–180 mmHg). Although the 24-h urinary-fractionated metanephrine (MN) and normetanephrine (NMN) (mg/day) was not normally distributed, the logarithmic representation (base = 10) of the combination of these continuous variables showed a normal distribution (p = 0.549). Log24 − h urinary−fractionated MN and NMN was correlated with intraoperative maximum AP (R = 0.481, p < 0.001). Multiple regression analyses showed that diabetes mellitus (β = 15.835, standard error [SE] = 7.550, t statistic = 2.097, P = 0.040), the classic triad (β = 14.081, SE = 5.668, t statistic = 2.484, P = 0.016), and log24 − h urinary−fractionated MN and NMN (β = 14.641, SE = 5.842, t statistic = 2.506, P = 0.015) were independent factors associated with intraoperative maximum AP. Patients with PPGL accompanied by diabetes mellitus, the classic triad, and high log 24−h urinary−fractionated MN and NMN values may be at risk for hypertensive crises during surgery regardless of whether preoperative α-receptor blockades are used. Clinicians should manage these patients more carefully and effectively.
Selection of an Appropriate Bedrock for Site Amplification Studies.
Authors: Pandey Bhavesh , Jakka Ravi S 
Published: September 2021

Abstract
The selection of half-space or reference sites significantly influences site amplification studies. However, there are no well-defined guidelines in the literature. Generally, a layer with a local shear wave velocity (VS) of more than 760 m/s is considered a bedrock/half-space/reference site. This study attempts to formulate a rationale for selecting bedrock stiffness to be used as a half-space/reference site. For this study, VS,30 (average shear wave velocity of top 30-meter soil strata from shear wave velocity measurements) and the site's fundamental frequency (obtained from Horizontal to vertical spectral ratio of ambient vibration records) were used as proxies to study the influence of bedrock/half-space and development of a rationale for their selection. This study uses strong-motion data from India's sixty-two strong motion stations and a few from Japan (Kik-Net). The results suggest that considering a site with a shear wave velocity of 760 m/s may not be suitable as a half-space/bedrock for most geomorphological conditions. The results also recognize a pattern that can help in the development of a mathematical model for determining the bedrock for a site using VS,30 and its fundamental frequency as a proxy.
Cellular heterogeneity of circulating CD4+CD8+ double-positive T cells characterized by single-cell RNA sequencing
Authors: Choi Sung Min , Park Hi Jung , Choi Eun A , Jung Kyeong Cheon , Lee Jae Il 
Published: September 2021

Abstract
Circulating CD4+CD8+ double-positive (DP) T cells are associated with a variety of disease states. However, unlike conventional T cells, the composition of this population is poorly understood. Here, we used single-cell RNA sequencing (scRNA-seq) to analyze the composition and characteristics of the DP T cell population circulating in the peripheral blood of cynomolgus monkeys. We found that circulating DP T cells not only contain a large number of naïve cells, but also comprise a heterogeneous population (Th1-, Th2-, Th17-, Tfh-, Treg-, Eomes+ Tr1-, CD4 CTL-, CD8 CTL-, and innate-like cells) with multiple potential functions. Flow cytometry analysis revealed that a substantial number of the naïve DP T cells expressed CD8αβ, as well as CD8αα, along with high expression of CD31. Moreover, the CD4hiCD8lo and CD4hiCD8hi populations, which express high levels of the CD4 coreceptor, comprised subsets characterized by helper and regulatory functions, some of which also exhibited cytotoxic functions. By contrast, the CD4loCD8hi population with high CD8 coreceptor expression comprised a subset characterized by CD8 CTL- and innate-like properties. Taken together, the data show that scRNA-seq analysis identified a more diverse subset of the circulating DP cells than is currently known, despite this population being very small.
Multiplexed identification of RAS paralog imbalance as a driver of lung cancer growth
Authors: Tang Rui , Shuldiner Emily , Kelly Marcus , Murray Christopher , Hebert Jess , Andrejka Laura , Tsai Min , Hughes Nicholas , Parker Mitchell , Cai Hongchen , Li Yao-Cheng , Wahl Geoffrey , Dunbrack Roland , Jackson Peter , Petrov Dmitri , Winslow Monte 
Published: September 2021

Abstract
Oncogenic KRAS mutations occur in approximately 30% of lung adenocarcinoma. Despite several decades of effort, oncogenic KRAS-driven lung cancer remains difficult to treat, and our understanding of the positive and negative regulators of RAS signaling is incomplete. To uncover the functional impact of diverse KRAS-interacting proteins on lung cancer growth in vivo, we used multiplexed somatic CRISPR/Cas9-based genome editing in genetically engineered mouse models with tumor barcoding and high-throughput barcode sequencing. Through a series of CRISPR/Cas9 screens in autochthonous lung tumors, we identified HRAS and NRAS as key suppressors of KRASG12D-driven tumor growth in vivo and confirmed these effects in oncogenic KRAS-driven human lung cancer cell lines. Mechanistically, RAS paralogs interact with oncogenic KRAS, suppress KRAS-KRAS interactions, and reduce downstream ERK signaling. HRAS mutations identified in KRAS-driven human tumors partially abolished this effect. Comparison of the tumor-suppressive effects of HRAS and NRAS in KRAS- and BRAF-driven lung cancer models confirmed that RAS paralogs are specific suppressors of oncogenic KRAS-driven lung cancer in vivo. Our study outlines a technological avenue to uncover positive and negative regulators of oncogenic KRAS-driven cancer in a multiplexed manner in vivo and highlights the role of RAS paralog imbalance in oncogenic KRAS-driven lung cancer.
Household quarantine of second degree contacts is an effective non-pharmaceutical intervention to prevent tertiary cases in the current SARS-CoV pandemic
Authors: Weigl Josef A. I. , Feddersen Anna-Katharina , Stern Mona 
Published: September 2021

Abstract
Background
Given the characteristics of SARS-CoV2 with regard to transmission before the onset of symptoms and varying manifestation indices according to age, isolation and quarantine have limited efficacy in the current pandemic. Household quarantine in second degree contacts (Hh-Q2°) outside the case household has so far only been addressed by modellers. In the literature there is no publication based on field data.
Methods
In a retrospective cohort study on real field data from a county health department (CHD), all PCR-confirmed cases and related contact persons put into quarantine were analysed. Hh-Q2° was used in our CHD from the onset of the pandemic.
Results
From 9 March to 8 December 2020, 353 PCR-confirmed cases were registered in the CHD Ploen, Northern Germany: 225 (63.7%) primary, 107 (30.3%) secondary and 21 (5.9%) tertiary cases. The 107 secondary cases resulted out of 470 (22.8%) close or 1°contacts and 21 tertiary cases out of 179 (11.7%) indirect or 2°contacts put into quarantine. The efficacy of Hh-Q2° was 51.5% (11.7%/22.8%) of that of quarantine in 1°contacts; 16.4% of all converted cases in quarantined persons were ascertained by Hh-Q2°. One in ten 1°contacts in households with tertiary cases remained asymptomatic.
Conclusion
The impact of Hh-Q2° in preventing further spread of SARS-CoV2 was considerable. With 51% of the conversion rate in 2°contacts compared to 1°contacts, the efficacy of Hh-Q2° is substantial. Hh-Q2° should definitely be used routinely to control the spread of SARS-CoV2 more efficiently and national authorities should include it in their guidelines.
Induction of robust cellular and humoral immunity against SARS-CoV-2 after a third dose of BNT162b2 vaccine in previously unresponsive elderly
Authors: Olmedo Addi Romero , Schulz Axel , Hochstäter Svenja , DasGupta Dennis , Virta Iiris , Hirseland Heike , Staudenraus Daniel , Camara Bärbel , Münch Carina , Hefter Véronique , Sapre Siddhesh , Mei Henrik , Keller Christian , Lohoff Michael 
Published: September 2021

Abstract
Herein, we compared SARS-CoV-2-specific antibody and T-cell responses to two doses of BNT162b2 mRNA in 51 vaccinees > 80 years old and 46 (20–53 years old) controls. The responses of the elderly were much lower and 10% non-responders were identified. Importantly, in four of them, a third vaccination raised the immune response to levels seen in responders after two vaccinations, thus implying that non-response is not fateful even in the elderly.
Analysis of cardiac involvement in patients recovered from Covid-19 without troponin elevation, evaluated by cardiovascular magnetic resonance.
Authors: Carlessi Adrian , Perello Leonel , Pantaley Cristian , Borsini Armando , Rossi Lucia , Gimenez Florencia , Leonardi Julian , Ballina Ariel , Maillo Martin , Rienzo Paula Di , Gonzalo Paula , Vara Martin Gonzalez , Vegetti Luciana , Wustten Sebastian , Costa Lucas , Vergara Diego , Froullet Cristian 
Published: September 2021

Abstract
Background

The disease caused by coronavirus (COVID-19) affects the cardiovascular system, whether by direct viral aggression or indirectly through systemic inflammation and multiple organ compromise. A widely used method to determine cardiac injury is troponin measurement. The aim of this study is to evaluate the prevalence of cardiac involvement (CINV) in a population recovered from COVID-19, referred to cardiac MRI (CMR), who did not present troponin elevation.
Methods

There were 156 patients that recovered from COVID-19 and who did not present troponin elevation referred to CMR. CINV was considered to be the presence of: late gadolinium enhancement (LGE), edema, myocarditis, pericarditis, left ventricular systolic dysfunction (LVSD) and/or depressed right ventricular systolic dysfunction (RVSD).
Results

Prevalence of CINV was 28.8%, being more frequent in men (p = 0.002), in patients who required hospitalization (p = 0.04) and in those who experienced non-mild cases of infection (p = 0.007). RVSD (17.9%) and LVSD (13.4%) were the most frequent findings. The rate of myocarditis was 0.6%. LGE manifested in 7.1% of patients and its presence was related to less left ventricular ejection fraction (LVEF) (p = 0.0001) and right ventricular ejection fraction (RVEF) (p = 0.04).
Conclusion

In patients who recovered from COVID-19, 28.8% of CINV was found. It was more frequent in men, in patients who required admission and in patients with cases of non-mild infection. The patients that presented LGE had less LVEF and RVSF.
Evidence of Plasmodium falciparum infection after 12 years from the exposure: cryptic malaria or recrudescence?
Authors: Patamia Ildebrando , Cappello Elisa , Calvo Maddalena , Migliorisi Giuseppe , Franco Antonina 
Published: September 2021

Abstract
BackgroundMalaria infections affect a high percentage of the world's population, especially in tropical and subtropical regions. Specifically, Plasmodium falciparum is the most relevant species involved in the etiopathogenesis of this infection. The duration of P. falciparum infections is often undefined, as some reported episodes of suspected recrudescence occur several years after initial exposure.Case presentationWe present a case report of malaria infection with low parasitaemia in a man whose last stay in an endemic region or contacts with the local population was twelve years ago. The patient recovered fully with adequate antimalarial treatment, but some aspects of his clinical history were not clearly defined.ConclusionsWe discuss here the possibility that this is either a P. falciparum recrudescence or an episode of cryptic malaria, as we cannot carefully verify some details of our patient's life and history.
Radiological pleuroparenchymal fibroelastosis-like lesion in idiopathic interstitial pneumonias
Authors: Fujisawa Tomoyuki , Horiike Yasuoki , Egashira Ryoko , Sumikawa Hiromitsu , Iwasawa Tae , Matsushita Shoichiro , Sugiura Hiroaki , Kataoka Kensuke , Hashisako Mikiko , Yasui Hideki , Hozumi Hironao , Karayama Masato , Suzuki Yuzo , Furuhashi Kazuki , Enomoto Noriyuki , Nakamura Yutaro , Inui Naoki , Suda Takafumi 
Published: September 2021

Abstract
BackgroundPleuroparenchymal fibroelastosis (PPFE) is characterised by predominant upper lobe pleural and subpleural lung parenchymal fibrosis. Radiological PPFE-like lesion has been associated with various types of interstitial lung diseases. However, the prevalence and clinical significance of radiological PPFE-like lesion in patients with idiopathic interstitial pneumonias (IIPs) are not fully understood. We aimed to determine the prevalence and clinical impact on survival of radiological PPFE-like lesion in patients with IIPs.MethodsA post-hoc analysis was conducted using data from the Japanese nationwide cloud-based database of patients with IIPs. All the patients in the database were diagnosed as having IIPs by multidisciplinary discussion. Patients diagnosed with idiopathic PPFE were excluded. Clinical data and chest computed tomography (CT) image of 419 patients with IIPs were analysed. The presence of radiological PPFE-like lesion was independently evaluated by two chest radiologists blind to the clinical data.ResultsOf the 419 patients with IIPs, radiological PPFE-like lesions were detected in 101 (24.1%) patients, mainly in idiopathic pulmonary fibrosis (IPF) and unclassifiable IIPs, but less in idiopathic nonspecific interstitial pneumonia. Prognostic analyses revealed that radiological PPFE-like lesion was significantly associated with poor outcome in patients with IIPs, which was independent of age, IPF diagnosis and %FVC. In survival analyses, the patients with radiological PPFE-like lesions had poor survival compared with those without (log-rank, p < 0.0001). Subgroup analyses demonstrated that radiological PPFE-like lesion was significantly associated with poor survival both in patients with IPF and those with unclassifiable IIPs.ConclusionRadiological PPFE-like lesion is a condition that could exist in IIPs, mainly in IPF and unclassifiable IIPs. Importantly, the radiological PPFE-like lesion is a non-invasive marker to predict poor outcome in patients with IIPs, which should be carefully considered in clinical practice.
Elevated circulating stem cells level is observed one month after implantation of Carmat bioprosthetic total artificial heart
Authors: Guyonnet Léa , Detriché grégoire , Gendron Nicolas , Philippe Aurélien , Latremouille Christian , Soret Lou , Capel Antoine , Peronino Christophe , Jansen Piet , Ivak Peter , Carpentier Alain , Mirault Tristan , Netuka Ivan , Guerin Coralie L , Smadja David 
Published: September 2021

Abstract
The Aeson® total artificial heart (A-TAH) has been developed as a total heart replacement for patients at risk of death from biventricular failure. We previously described endothelialization of the hybrid membrane inside A-TAH probably at the origin of acquired hemocompatibility. We aimed to quantify vasculogenic stem cells in peripheral blood of patients with long-term A-TAH implantation. Four male adult patients were included in this study. Peripheral blood mononuclear cells were collected before A-TAH implantation (T0) and after implantation at one month (T1), between two and five months (T2), and then between six and twelve months (T3). Supervised analysis of flow cytometry data confirmed the presence of the previously identified Lin−CD133+CD45− and Lin−CD34+ with different CD45 level intensities. Lin−CD133+CD45−, Lin−CD34+CD45− and Lin−CD34+CD45+ were not modulated after A-TAH implantation. However, we demonstrated a significant mobilization of Lin−CD34+CD45dim (p = 0.01) one month after A-TAH implantation regardless of the expression of CD133 or c-Kit. We then visualized data for the resulting clusters on a uniform manifold approximation and projection (UMAP) plot showing all single cells of the live Lin− and CD34+ events selected from down sampled files concatenated at T0 and T1. The three clusters upregulated in T1 are CD45dim clusters, confirming our results. In conclusion, using a flow cytometry approach, we demonstrated in A-TAH-transplanted patients a significant mobilization of Lin−CD34+CD45dim in peripheral blood one month after A-TAH implantation.
Trust-Enabled Energy Effective Optimal Framework for Detection of Intrusions in the Network Using AI Scheme
Authors: Srivi Putty , Devi Lavadya Nirmala 
Published: September 2021

Abstract
Wireless Sensor Networks (WSN) are self-possessed of the devices that are capable of actuating/sensing, processing, and communicating. This is employed for enhancing the day-to-day life, moreover secure data transmission was regarded as the major challenging aspect for the deployment of data. Data dissemination is a crucial in the complex communications framework for transferring messages for any given condition on the network. The dilemma of fixing the safest efficient route was a tedious issue. Hence the secure and most reliable way will give the appropriate solution for the routing issues. Here in this paper the Trust based energy efficient route path identification by Multi-faceted biologically-inspired probabilistic Cuckoo search Node optimization algorithm (TEERP-MFBPCS)is employed to find the efficient safest route within a short period. After seeing the efficient route, the node can be distinguished upon the traffic and security. Then in the selected route, the nodal distance can be calculated through applying weighted-biased end-to-end delay-based approach for traffic analysis. Finally, the intrusion node can be detected and the performance analysis is carried.
Promising Strategies to Support COVID-19 Vaccination of Healthcare Personnel: Insights from the VHA National Implementation
Authors: Giannitrapani Karleen F. , Brown-Johnson Cati , Connell Natalie B. , Yano Elizabeth M. , Singer Sara J. , Giannitrapani Susan N. , Thanassi Wendy , Lorenz Karl A. 
Published: September 2021

Abstract
Background
As of August 2021 up to 30% of Americans were uncertain about taking the COVID-19 vaccine. Some healthcare personnel (HCP) also delayed or declined vaccination.
Objective
Identify barriers and facilitators of Veterans Health Administration (VHA) HCP vaccination program
Design:
Key informant interviews with employee occupational health (EOH) providers
Participants:
38 VHA EOH providers representing 26 of VHA’s regionally diverse healthcare systems.
Approach:
Thematic analysis elucidated 5 key themes, and specific strategies recommended by EOH
Key Results:
Implementation themes included: 1) Leverage diverse skillsets through multidisciplinary effort, specifically COVID-19 vaccination teams with clear goals/roles. 2) “Focus like a laser”: invest in processes and align resources with priorities, including specific strategies of: creating detailed processes, eg. logistics plan to prevent wastage and allocate excess vaccine doses; addressing time trade-offs for personnel involved in vaccine clinics by suspending everything non-essential; designating process/authority to shift personnel where needed; and proactively involving leaders to support resource allocation/alignment. 3) Expect and accommodate vaccine buy-in occurring over time, including specific strategies of: preparing for some HCP slow buy-in; aligning buy-in facilitation with identities and motivation; encouraging word-of-mouth and hyper-local testimonials. 4) Overcome misinformation through trustworthy communication, with specific strategies including: tailoring communication to individuals and addressing COVID vaccines “in every encounter”; leveraging proactive institutional messaging (e.g., townhalls, Q&As) to reinforce information; inviting bi-directional conversations about hesitancy. 5) Use existing and newly developed communication channels to foster sharing and learning across teams and sites, eg. a national VHA EOH listserv.
Conclusions
Expecting deliberation allows systems to prepare for complex distribution logistics, and conversations that are trustworthy, bi-directional, and identity-aligned - overall supporting mandate goals. Ideally, organizations 1) provide time for conversations about vaccines; those conversations would 2) address individual concerns and foster bi-directional shared decision-making, 3) be informed by identity-based motivation, and 4) delivered by identity-concordant individuals.
Application of interdisciplinary collaborative hospice care for terminal geriatric cancer patients: A prospective randomised controlled study
Authors: LIU Yong , Shen Yin , Pan Qinghua , Zou Houwen , Huang Zuochao , Liu Zhiping , Wang Xiaomao 
Published: September 2021

Abstract
Background
Hospice care (HC) is specialized medical care for terminal patients who are nearing the end of life. Interdisciplinary collaborative hospice care (ICHC) is where experts from different disciplines and patients/caregivers form a treatment team to establish shared patient care goals. However, the ICHC efficacy has not been frequently studied in the terminal geriatric cancer patients (TGCPs) population. This study aimed to gain insight into ICHC provided to TGCPs by an ICHC team and identify factors to ameliorate multidimensional HC.
Methods
166 TGCPs were equally divided into ICHC group and life-sustaining treatments (LSTs) group as control. The scores of these questionnaires [such as EORTC QLQ-C30, Hamilton Anxiety Scale], the median survival time (MST), symptoms improvement, the median average daily cost of drugs (MADDC), the median total cost of drugs (MTDC) in the last 2 days, and medical care satisfaction were observed in both groups.
Results
After treatment, the emotional function and symptoms in the ICHC group were statistically higher improvement than those in the LSTs group (P < 0.05). The MADDC and the MTDC in the last 2 days were statistically lower in the ICHC group than those in the LSTs group (P < 0.01). In addition, the overall satisfaction situation and the cooperation ability in the ICHC group were statistically higher than those in the LSTs group (P < 0.01).
Conclusion
The ICHC could provide TGCPs with coordinated, comfortable, high-quality, and humanistic care.