bluuebunny/crossref_metadata_2025_split huggingface dataset:
FOXA3: A Novel Tumor Suppressor in Esophageal Squamous Cell Carcinoma
Authors: Zhang Siang , Jin Yuxiang , Han Qianyu , Zhao Xuewei , Xue Lei 
Published: February 2025

ABSTRACTBackgroundThe forkhead box A (FOXA) family has been extensively studied in cancer research; however, the role of FOXA3 in malignant tumors, particularly esophageal squamous cell carcinoma (ESCC), is not well understood. This study explores the expression and function of FOXA3 in ESCC, assessing its potential as a prognostic marker and therapeutic target.MethodsThis study analyzed FOXA3 expression in ESCC tissues and its correlation with patient prognosis. The effects of FOXA3 overexpression on ESCC cell proliferation, migration, and invasion were examined in ESCC cell lines in vitro. Additionally, an in vivo tumorigenesis assay was performed using subcutaneous injection to assess the impact of FOXA3 overexpression on tumor growth. Statistical analyses were conducted to determine the significance of the results.ResultsFOXA3 expression was significantly reduced in ESCC tissues compared with it in paired adjacent normal tissues, and low FOXA3 expression was significantly associated with poor prognosis in ESCC patients. FOXA3 overexpression markedly inhibited ESCC cell proliferation, migration, and invasion. In addition, overexpression of FOXA3 repressed tumor growth in mice.ConclusionsThese findings indicate that FOXA3 acts as a tumor suppressor in ESCC, and its low expression is linked to poor outcomes. FOXA3 may serve as a potential diagnostic and therapeutic target for ESCC.
Betulinic Acid Inhibits Glioma Progression by Inducing Ferroptosis Through the PI3K/Akt and NRF2/HO‐1 Pathways
Authors: Huang Jinxiang , Li Qixuan , Wang Hongxiang , Zhang Shuai , Zhou Xiaorong , Huang Fang , Chen Juxiang , Xu Luning , Kang Dezhi 
Published: February 2025

ABSTRACTBackgroundGliomas currently have a poor prognosis and limited therapy options. Betulinic acid (BA) has demonstrated antitumor activity in various cancers. This study is aimed at clarifying the underlying mechanisms by which BA inhibits gliomas.MethodsWe assessed how BA affected the migration, apoptosis, invasion, proliferation, and viability of U251 glioma cells. The genes that were differentially expressed after BA treatment were identified via RNA sequencing. Utilizing Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes, research was done to determine the affected pathways. Molecular docking was applied to explore the interaction of BA with key pathway molecules. Experimental assays were conducted to confirm the impact of BA on these pathways and targets.ResultsIn U251 cells, BA reduced viability; inhibited colony formation, migration, and invasion; and triggered apoptosis. Through RNA sequencing, 923 up‐ and 1469 downregulated genes were found, with notable enrichment in the TNF, PI3K‐Akt, and ferroptosis pathways. BA can stably bind to TNF and PI3K‐Akt pathway molecules, especially AKT1 (binding energy = −10.2 kcal/mol). BA administration decreased the levels of phosphorylated PI3K and AKT. Moreover, BA‐induced ferroptosis and HO‐1 and NRF2 levels were increased. Ferrostatin‐1 and zinc protoporphyrin pretreatment decreased intracellular iron and lipid peroxidation and decreased the decrease in cell viability caused by BA.ConclusionsBA controls the PI3K/Akt and NRF2/HO‐1 pathways, which results in glioma ferroptosis. Understanding BA's multipathway mechanism may inform its therapeutic potential in glioma treatment.
Mass Spectrometry–Based Proteomics in Clinical Diagnosis of Amyloidosis and Multiple Myeloma: A Review (2012–2024)
Authors: Kratka Katerina , Sistik Pavel , Olivkova Ivana , Kusnierova Pavlina , Svagera Zdenek , Stejskal David 
Published: February 2025

ABSTRACTProteomics is nowadays increasingly becoming part of the routine clinical practice of diagnostic laboratories, especially due to the advent of advanced mass spectrometry techniques. This review focuses on the application of proteomic analysis in the identification of pathological conditions in a hospital setting, with a particular focus on the analysis of protein biomarkers. In particular, the main purpose of the review is to highlight the challenges associated with the identification of specific disease‐causing proteins, given their complex nature and the variety of posttranslational modifications (PTMs) they can undergo. PTMs, such as phosphorylation and glycosylation, play critical roles in protein function but can also lead to diseases if dysregulated. Proteomics plays an important role especially in various medical fields ranging from cardiology, internal medicine to hemato‐oncology emphasizing the interdisciplinary nature of this field. Traditional methods such as electrophoretic or immunochemical methods have been mainstay in protein detection; however, these techniques are limited in terms of specificity and sensitivity. Examples include the diagnosis of multiple myeloma and the detection of its specific protein or amyloidosis, which relies heavily on these conventional methods, which sometimes lead to false positives or inadequate disease monitoring. Mass spectrometry in this respect emerges as a superior alternative, providing high sensitivity and specificity in the detection and quantification of specific protein sequences. This technique is particularly beneficial for monitoring minimal residual disease (MRD) in the diagnosis of multiple myeloma where traditional methods fall short. Furthermore mass spectrometry can provide precise typing of amyloid proteins, which is crucial for the appropriate treatment of amyloidosis. This review summarizes the opportunities for proteomic determination using mass spectrometry between 2012 and 2024, highlighting the transformative potential of mass spectrometry in clinical proteomics and encouraging its wider use in diagnostic laboratories.
Comprehensive Analysis of Clinical and Molecular Features in Cancer Patients Associated With Major Human Oncoviruses
Authors: Mohamed Ahmed Osman , Long Rongzhuo , He Yin , Wang Xiaosheng 
Published: February 2025

ABSTRACTViral infections contribute to a higher incidence of cancer than any other individual risk factor. This study aimed to compare the clinical and molecular features of four viral‐associated cancers: stomach adenocarcinoma (STAD), head and neck squamous cell carcinoma (HNSC), liver hepatocellular carcinoma (LIHC), and cervical squamous cell carcinoma (CESC). Patients were categorized based on viral infection status, as provided in the clinical data, into virus‐associated and non‐virus‐associated groups, followed by a comprehensive comparison of clinical and molecular features. Our analysis disclosed that viral infections confer unique clinical and molecular signatures to their associated tumors. Specifically, human papillomavirus‐associated (HPV+) HNSC and hepatitis B virus‐associated (HBV+) LIHC patients were predominantly male, younger, and exhibited better clinical prognoses. Virus‐associated tumors displayed enhanced immune microenvironments and high DNA damage response scores, while non‐virus‐associated tumors were enriched in stromal signatures. HPV+ HNSC and Epstein‐Barr virus‐associated (EBV+) STAD showed similarities across multi‐omics features, including better responses to immunotherapy, lower TP53 mutation rates, tumor mutation burden (TMB), and copy number alteration (CNA). Conversely, HBV+, Hepatitis C virus‐associated (HCV+) LIHCs and HPV+ CESC were more genomically unstable due to high TP53 mutation rates, TMB, and CNA. At the protein level, Caspase‐7 and Syk were upregulated in HPV+ HNSC and EBV+ STAD, and positively correlated with the enrichment levels of CD8 + T cell, PD‐L1, and cytolytic activity. Patient stratification based on infection status has significant clinical implications, particularly for patient prognosis and drug response.
I Remember It All Too Well: Gig Workers' Psychological Detachment After Receiving Negative Customer Feedback and the Roles of Job Security and Handling Time
Authors: Liang Yijue , Sun Tianjun , Zhu Ze 
Published: February 2025

ABSTRACTThe recently booming gig economy, in which gig workers provide on‐demand and short‐term services to customers in exchange for monetary rewards via online gig platforms, creates opportunities for workers to receive instantaneous customer feedback, particularly negative feedback. Considering the emerging controversy concerning gig workers' well‐being, the present research strives to understand how and when negative customer feedback influences gig workers' subsequent work and well‐being outcomes. Grounded in conservation of resources (COR) theory, this research hypothesizes that negative customer feedback hinders psychological detachment, contingent on gig workers' perception of their job security and their customer feedback handling time. In turn, impaired psychological detachment is related to lower job performance and job‐related well‐being for gig workers on the following day. Findings from a study using an experience sampling method (ESM) that included 147 gig workers across five consecutive workdays (Study 1) and a recall study that included 273 gig workers (Study 2) provide general support for the hypothesized model. The theoretical and practical implications of this research are also discussed.
Ascorbic and silicic acid application mitigated toxic effects of ozone in mung bean (Vigna radiataL. Wilczek) by modulating growth, secondary metabolites, water relations, and grain quality attributes
Authors: Iqbal Naeem , Shahzadi Eram , Nawaz Muhammad , Shahid Muhammad , Khan Fahad 
Published: February 2025

AbstractBACKGROUNDElevated levels of tropospheric ozone (O3) pose a significant threat to plant health and productivity. Developing ozone‐tolerant varieties is crucial for mitigating these environmental stresses. This study investigates the effects of ascorbic acid (AA) and silicic acid (SA) treatments on 12 different mung bean varieties under elevated O3 conditions.RESULTSA controlled pot experiment was conducted with four treatments: ambient O3 (40–45 ppb), elevated O3 (120 ppb), elevated O3 with silicic acid (0.1 mmol L−1), and high O3 with ascorbic acid (10 mmol L−1). High O3 stress negatively impacted growth attributes across all mung bean cultivars. However, both AA and SA treatments significantly alleviated O3‐induced growth reductions. Under O3 stress, osmotic potential, water potential, relative water content, turgor potential, sugars, pod number, amino acids, 100‐seed weight, and grain carbohydrates all decreased. In contrast, antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, and superoxide dismutase), flavonoids, tannins, and grain protein content increased.CONCLUSIONThe NIAB Mung 20‐21, NIAB Mung 2006, and NIAB Mung varieties exhibited O3 resistance. Silicic acid proved to be more effective than ascorbic acid in mitigating O3 damage, though a combination of both treatments was the most beneficial for enhancing plant resilience under elevated O3 conditions. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Development of a Catalyst‐Free Ultrasound‐Assisted Derivatization Method for Detection of Valproic Acid in Epilepsy Patient's Serum Using High‐Performance Liquid Chromatography: A Comparison With Chemiluminescence Immunoassay
Authors: Jaberi Javad , Alavi Nikoo , Moayednia Milad 
Published: February 2025

ABSTRACTThe aim of this research was the therapeutic drug monitoring for valproic acid in epilepsy patient's serum samples by the common, sensitive, and accessible HPLC‐UV method. Because of the absence of a suitable chromophore in the valproic acid structure, a facile, selective, and cost‐effective pre‐column derivatization was designed. This catalyst‐free ultrasound‐assisted derivatization assay can accomplish the derivatization very quickly only in 5.0 min and at a mild temperature of 60°C. 2,4ʹ‐Dibromoacetophenone and nonanoic acid was used as derivatizing agent and internal standard, respectively. The effect of sample pH, buffer concentration, ultrasound exposure time, reaction temperature, and derivatizing agent amount were optimized. The proposed method exhibited a good linear range of 5.0–300.0 µg/mL with acceptable correlation coefficients of 0.9981. The limit of detection was as low as 0.4 µg/mL. Also, the limit of quantification was reported as 1.3 µg/mL. Interday and intraday relative standard deviations (n = 10) were 1.1% and 0.3%–7.0%, respectively. In addition, the relative recovery ranged from 100.3% to 107.7%. The measurement of valproic acid was performed in the presence of several epilepsy and non‐epilepsy drugs by the developed protocol. This confirmed the specific and accurate determination of valproic acid in the patient's serum. A comparative evaluation was employed against the precise chemiluminescence immunoassay approach. The correlation coefficient between the two methods was 0.9992, which demonstrated the results were statistically the same.
Chiral Separation and Determination of Multiple Organophosphorus Pesticide Enantiomers in Soil Based on Cellulose‐Based Chiral Column by LC–MS/MS
Authors: Li Liang , Zhou Rendan , Xie Huiying , Li Gang , Xu Zhiguang , Liu Meijia , Gao Wanting 
Published: February 2025

ABSTRACTThe widespread use of organophosphorus pesticides (OPs) has raised significant environmental and health concerns due to their residues in soil and potential entry into the food chain. This study introduced chiral analysis methods for four OPs—methamidophos (METHP), dipterex (DIP), malathion (MALA), and isothiophos‐methyl (ISOME)—using liquid chromatography–tandem mass spectrometry (LC–MS/MS) with cellulose‐based chiral columns. Three distinct methods were established: one for METHP, another for DIP, and a third for MALA and ISOME. Key chromatographic variables, including organic mobile phases and column temperatures, were systematically optimized, achieving maximum resolutions (Rs) of 1.61 for METHP, 2.40 for DIP, 1.70 for MALA, and 2.02 for ISOME. The QuEChERS method was employed for sample pretreatment, ensuring high recoveries. All three methods demonstrated excellent linearity (R > 0.998), accuracy with recoveries ranging from 79% to 121%, precision with RSD% < 11%, and sensitivity with low limits of enantiomer detection (LODs) as low as 0.17 µg/kg for METHP, 0.087 µg/kg for DIP, 0.062 µg/kg for MALA, and 0.054 µg/kg for ISOME, representing a sensitivity improvement of 16–172 times compared to existing methods. Field soil samples from Yangzizhou District, Nanchang, China, revealed significant contamination by ISOME, with concentrations of a single enantiomer reaching up to 8343 µg/kg, while MALA exhibited varying enantiomeric ratios with depth. This study provides robust analytical tools for monitoring chiral OP residues in soil, contributing to food safety and environmental protection.
Differences in the Sonographic Features of Adenomyosis and Concurrent Endometriosis Compared to Isolated Adenomyosis
Authors: Matot Ran , Blickstein Ophir , Leibner Gideon , Bar‐Peled Uval , Borovich Adi , Geron Yossi , Gilboa Yinon , Krissi Haim , Perlman Sharon 
Published: February 2025

ObjectiveTo examine whether the co‐occurrence of endometriosis affects the sonographic features of adenomyosis based on the revised Morphological Uterus Sonographic Assessment (MUSA) criteria.MethodsThis prospective cohort study utilized data from a tertiary referral center collected between 2010 and 2022. Non‐pregnant women aged 20–53 years who presented with symptoms potentially related to adenomyosis and underwent pelvic ultrasound scans were included. Diagnoses were based on the revised MUSA criteria, which distinguish between direct features (endometrial cysts, hyperechogenic islands, echogenic sub‐endometrial lines, and buds) and indirect features (globular shape of the uterus, asymmetrical uterine wall thickening, irregular junctional zone, fan‐shaped shadowing, translesional vascularity, and interrupted junctional zone). Patients were categorized into 2 groups: 1) concurrent adenomyosis and endometriosis and 2) isolated adenomyosis. Demographic and clinical characteristics were retrospectively collected.ResultsNinety‐four patients were diagnosed with adenomyosis. Of these, 24 (27%) had concurrent endometriosis, while 70 had isolated adenomyosis. The most frequent sonographic features were globular uterine configuration (52%), myometrial cysts (44%), and asymmetrical myometrial thickening (33%). The isolated adenomyosis group had a higher proportion of direct features (29%) and both direct and indirect features (33%) compared to the concurrent group, which predominantly exhibited indirect features (71%) (P < .05). Direct features of myometrial cysts were significantly more frequent in the isolated adenomyosis group (51%) compared to the concurrent group (21%, P = .01).ConclusionsUtilizing the revised MUSA criteria revealed significant differences in the sonographic features of adenomyosis in symptomatic patients with concurrent endometriosis compared to isolated adenomyosis. This highlights the necessity for standardized diagnostic methods and enhances understanding of the complex relationship between adenomyosis and endometriosis, underscoring the importance of accurate diagnosis in clinical practice.
IGF2BP2‐induced circRNF20 facilitates breast cancer cell proliferation via the HuR/CDCA4 axis
Authors: Wu Shu‐Tao , Hou Xiao‐Li , Wang Fei , Sun Wei , Chen Jia‐Jie , Cao Ya‐Sen , Cheng Hong 
Published: February 2025

AbstractThis study aimed to explore the mechanism of insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) affecting the proliferation of breast cancer (BC) cells. The expression of IGF2BP2, circRNA ring finger protein 20 (circRNF20), and cell division cycle‐associated protein 4 (CDCA4) in human BC cells and normal breast epithelial cells was detected via RT‐qPCR or Western blotting. After IGF2BP2 expression was altered, CCK‐8 assay, colony formation assay, and EdU staining were performed to evaluate changes in the proliferation of BC cells. RNA immunoprecipitation (RIP) assay was used to analyze the binding of circRNF20 to IGF2BP2 or HuR, as well as the binding of HuR to CDCA4. RNA pull‐down confirmed the interaction between circRNF20 and HuR. The stability of circRNF20 was tested after treatment with actinomycin D. A nude mouse xenograft tumor model was established to validate the effect of IGF2BP2 in vivo. IGF2BP2, circRNF20, and CDCA4 were highly expressed in BC cells. Silencing IGF2BP2 decreased the proliferation ability of BC cells. Mechanistically, the binding of IGF2BP2 to circRNF20 prevented circRNF20 degradation, thereby promoting the binding of circRNF20 to HuR and increasing the expression of CDCA4. The overexpression of circRNF20 or CDCA4 abolished the inhibitory effect of IGF2BP2 silencing on BC cell proliferation. In conclusion, the binding of IGF2BP2 to circRNF20 prevents its degradation, thus facilitating BC cell proliferation via the HuR/CDCA4 axis.
China's Hydropower Resettlement Policies: Evolving Strategies for Sustainable Resource Management and Climate Security
Authors: Li Qian , Shangguan Ziheng , Yang Bohan , Xu Yi 
Published: February 2025

ABSTRACTThis study investigates the evolution of China's hydropower resettlement policies with the framework of sustainable resource management and climate security. Using text analysis and the policy modeling consistency (PMC) model, it evaluates policy adaptation to the socioeconomic impacts of large‐scale hydropower development, particularly the displacement of land‐losing farmers. The findings reveal a transition in policy focus from broad initial coverage to targeted prioritization in later stages, characterized by a “inverted‐U” trend in the PMC index and a “U‐shaped” variance across policy dimensions. The transitions has improved goal‐setting, transparency, and procedural justice, yet challenges persist in fostering public participation and ensuring equitable resource distribution. China's centralized governance model has proven effectiveness in policy implementation but often constrains stakeholder engagement. Drawing lessons from targeted poverty alleviation programs, this study underscores the need for a shift from traditional administrative approaches to integrated sociological governance. Additionally, the research highlights climate challenges faced by hydropower resettlement and advocates for enhanced ecological compensation measures and improved energy efficiency in resettlement areas. These findings contribute to advancing the equity, sustainability, and global applicability of resettlement policies in addressing food, water, and climate security.
Metasurface‐Integrated Organic Light‐Emitting Diodes: Analysis of Spectral Effects on Theoretical and Experimental Performance
Authors: Kang Kyungnam , Yoo Hajun , Lee Hyunwoong , Ka Sukhyeon , Kim Jaeyun , An Myungchan , Kim Young Gu , Suh DuckJong , Kim Sunghan , Kim Jungho , Kim Donghyun 
Published: February 2025

AbstractOrganic light‐emitting diodes (OLEDs) have emerged as a leading display technology and become rapidly embraced in commercial applications. In this paper, we explore the integration of metasurface (MS) designs for polarizer‐free top‐emitting OLEDs (TOLEDs) with enhanced absorption of ambient light. From the performance evaluated by varying the geometrical parameters of the MS, it is found that the broadband absorption in the long wavelength range, associated with the localized surface plasmon mode of the MS, can be tuned by adjusting these parameters. Optimizing these parameters can effectively localize and enhance near‐fields at the MS edges, thereby reducing the reflectance of MS‐integrated TOLEDs (MI‐TOLEDs) under ambient light. An optimized MS may balance reduced reflectance with minimal emission loss from TOLEDs. Numerical results suggest that MI‐TOLEDs can improve spectral out‐coupled light intensity and overall angular enhancement of the out‐coupled power by at least 50% and 45%, respectively, over conventional TOLED. In the whole visible wavelength range, the MI‐TOLED shows 48% lower reflectance compared to conventional TOLED, which is confirmed by experimental data measured from the MS optical cavity. Changes in encapsulation thickness influence the surface plasmon polariton mode, shifting resonance conditions in the reflectance spectra within the short wavelength range.
Scalable Fabrication of Self‐Reinforced Bioplastic Composites Using Short Fiber Reinforcements
Authors: Aziz Shazed , Colwell John , Heidarian Pejman , Mathel Vincent , Gauthier Emilie , McNally Tony , Peijs Ton , Varley Russell J. , Halley Peter J. , Vandi Luigi‐Jules 
Published: February 2025

AbstractBioplastics and biocomposites are eco‐friendly alternatives to their petrochemical derived commodity material, but tend to have inferior mechanical and thermal properties. In this work, short‐fiber self‐reinforced bioplastic composites (SRBCs) have been developed that seek to overcome some of these shortcomings. The SRBCs leverage melt‐spun drawn poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) fibers with axially‐oriented crystalline structures that exhibit a ≈6.7 °C higher melt temperature than the same PHBV in isotropic form. This enables a controlled‐temperature compounding process that preserves the crystalline structure of the fibers without distortion and ensures uniform distribution within the matrix. The resultant composites display a ≈35% increase in ultimate tensile strength and a ≈55% increase in impact resistance compared to neat PHBV polymer. This monolithic‐type composite system, characterized by high interfacial compatibility and strong fiber‐matrix adhesion, also supports high‐value recycling while preserving its mechanical properties across multiple lifecycle uses. By focusing upon discontinuous short fiber reinforcement, this work provides unprecedented opportunities for scaling SRBCs through commodity application pathways such as injection molding, compression molding, and 3D printing.