bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of myocyte‐enriched calcineurin‐inhibiting protein (MCIP1) in estrogen mediated repression of cardiac hypertrophy (795.5)
Authors: Turpin Courtney , Waikel Rebekah 
Published: June 2021

Premenopausal women have a lower incidence of left ventricular hypertrophy (LVH) and heart failure, which is thought to be the result of estrogen signaling. However, the mechanism for estrogen’s cardioprotective effects is not well understood. We seek to define the role of MCIP1, in estrogen‐mediated inhibition of LVH. It is known that estrogen induces the expression of MCIP1, which inhibits prohypertrophy protein calcineurin. In our study, LVH was surgically induced in 10‐12 week old mice using transverse aortic constriction (TAC). Two weeks post‐surgery, hearts were excised and total RNA was extracted from the apex of the heart for gene analysis. Given that estrogen is known to induce MCIP1, we expected MCIP1 levels to be higher in females. Surprisingly, mRNA levels of MCIP1 in SHAM females were 50% that of males and TAC levels of MCIP1 decreased in females to 20% of their SHAM value. To better understand regulation and the role of MCIP1 in estrogen mediated inhibition of hypertrophy, we are currently performing a series of in vitro experiments using neonatal cardiomyocytes exposed to phenylephrine to induce hypertrophy in the presence or absence of estrogen. Gene expression of MCIP1 and known modulators, including targeting miRNAs are being assessed at the mRNA and protein levels. Together these studies will define MCIP1’s role in estrogen repression of cardiac hypertrophy.Grant Funding Source: NIH 5P20RR016481‐12 8P20GM103436‐12, R15HL108192‐02, R25GM102776
Preclinical efficacy of the dual sigma receptor antagonist dopamine uptake inhibitor, CM699, as a medication for stimulant abuse (803.3)
Authors: Katz Jonathan , Hiranita Takato , Lopez Jessica , Brellenthin Angelique , Mesangeau Christopher , Alsharif Walid , Kopajtic Theresa , Coggiano Mark , Jamalapuram Seshulatha , Tsai Shang‐Yi , Avery Bonnie , Su Tsun‐Ping , Tanda Gianluigi , McCurdy Christopher 
Published: June 2021

Sigma receptor (σR) antagonists block many effects of stimulants, but not reinforcing effects assessed with self‐administration (SA). Yet, a recent study suggests that σR antagonism with dopamine transporter (DAT) inhibition selectively blocks stimulant SA. A compound with potential for dual σR‐DAT inhibition was synthesized by treating 1‐methyl‐2‐benzimidazolinone with 1,4‐dibromobutane in the presence of K2CO3 in dimethylformamide (DMF), yielding a compound that was then coupled with 3H‐spiro[isobenzofuran‐1,4'‐piperidine] in the presence of K2CO3 in DMF. The resulting compound, CM699, had affinities of 14, 2.30 and 318 nM at σ1Rs, σ2Rs and DAT, respectively. Antagonism at σ1Rs was confirmed by Western blots of σ1R‐EYFP bound to the ER chaperone, Binding immunoglobulin Protein (BiP). CM699 had no effect whereas the agonist, (+)‐pentazocine decreased BiP association. CM699 (5.0 mg/kg, iv) in rats produced a Cmax of 1.84 µg/mL in plasma, 5 min after injection which declined exponentially (overall T½ = 4.4 hr). CM699 produced dose‐dependent insurmountable and selective antagonism of cocaine SA in rats (ED50 = 3.99 mg/kg, ip) and blunted effects of cocaine on dopamine (DA) levels in the rat nucleus accumbens, indicating minimal abuse liability. The results provide preclinical proof of concept for dual σR/DAT inhibition as a novel approach for the development of medications to treat stimulant abuse.Grant Funding Source: NIH Intramural Research Program
Plasma proteomes associated with status of vitamins A and E in school aged Nepalese children (804.12)
Authors: West Keith , Cole Robert , Schulze Kerry , Ruczinski Ingo , Betz Joshua , Yager James , Groopman Joihn , Wu Lee , Christian Parul 
Published: June 2021

Vitamin deficiencies afflict undernourished populations, but remain latent from public health view and lack evidence‐guided interventions due to difficulty and cost of assessment. Plasma proteomics may offer a future approach to identify nutrient‐linked biomarkers, modeled to estimate status and predict prevalence of multiple vitamin deficiencies (Cole et al J Nutr 2013). We measured concentrations of retinol and α‐tocopherol (α‐T) by HPLC and relative abundance of plasma proteins by iTRAQ‐labeled, tandem mass spectrometry in plasma of 500, rural Nepalese children 6‐8 yr of age. Among ~980 proteins quantified in >10% of subjects, 77 were correlated with retinol (q <0.01, p<0.0006) and 42 with α‐T (q<0.01, p<0.0002), which included (Gene Symbols) RBP4, TTR, and many APO lipoproteins and novel correlates. Using linear mixed effects models, relative abundance of 3 proteins (RBP4, C1R and SCAF11) predicted serum retinol (R2=~82%) and 6 proteins (APOC3, APOB, PKM, RGS8, LRRC47 and FOX04) predicted α‐T status (R2=~72%); however, each nutrient had >9 other candidate protein predictors to explore further. A predictive plasma proteome may exist to assess vitamins A and E status and prevalence of deficiencies in populations if candidate proteins undergo absolute quantification.Grant Funding Source: Supported by the Bill and Melinda Gates Foundation Grants OPP5251 and GH614
Food security, produce intake/behaviors, and cervical health of adult women living in rural Appalachia: a pilot study (805.16)
Authors: Hewage Sumali , Griswold Hannah , Sergeev Alexander , Gerome Jody , Hamilton Angela , Holben David 
Published: June 2021

Background Low household food security (HFS) and high cervical cancer rates have been reported in Appalachian Ohio. High antioxidant intake may reduce the risk of human papillomavirus (HPV) infection and progression to cervical cancer.Study Objective To examine interrelationship of HFS, produce intake/ behaviors, and cervical health in adult women living in rural Appalachia.Method Survey of females 蠅 21 years [USDA HFS module, produce intake and behavior‐related instrument, and Risky Sexual Behavior (RSB) Index questionnaire]. Clinical HPV results were obtained.Results Participants (n=153) [HPV‐negative, n=139 (91.4%); HPV‐positive, n=13 (8.6%)] were 29.4±7.8 years. For HFS, 113 (75.3%) had high and 37 (24.7%) had marginal, low, or very low HFS. Statistically significant test results showed food insecure group had a higher RSB scores compared to their counterparts (p<0.001). RSB scores were statistically significantly higher in HPV‐positive group than HPV‐negative group (p=0.021). Test statistics provided a significant difference between food secure and food insecure groups in median perceived diet quality scores 78.68 and 59.57 respectively, p=0.014.Conclusion Food insecurity was associated with high RSB score and low perceived diet quality. Further studies are required.
Fishing for food: evaluating the impact of food‐producing livelihoods on food consumption and food security (805.8)
Authors: Fiorella Kathryn , Hickey Matt , Salmen Charles , Mattah Brian , Magerenge Richard , Brashares Justin , Fernald Lia 
Published: June 2021

Food‐producing livelihoods have the potential to improve nutrition, through direct consumption or indirectly through income. Yet, livelihood interventions rarely show any effects on nutrition indicators. In this study, we explore whether fishing households eat more fish and have higher food security than non‐fishing households around Lake Victoria, Kenya. In 2010, we conducted a household survey of 111 randomly sampled households in a rural fishing community where half of all households fish. Our results suggest that households that fish have higher fish consumption in bivariate analyses (results were attenuated in multi‐variate models). Households that fish were less likely to be food insecure, but the significance of the effect was compromised with the inclusion of socio‐economic variables. Our results suggest that socio‐economic factors may be more important than participation in food‐producing livelihoods with regard to consumption of high quality foods. The results add to the literature examining the ways in which household livelihoods interact with nutrition, and could have important implications for improving intervention effectiveness, particularly for nutrition‐related outcomes.Grant Funding Source: Supported by NSF‐GEO grant #115057
Associations of parental monitoring and weight concern on body esteem of normal weight and overweight 1st grade children (808.5)
Authors: Shriver Lenka , Hubbs‐Tait Laura , Harrist Amanda , Topham Glade , Page Melanie 
Published: June 2021

Prevention of body dissatisfaction is critical for minimizing adverse effects of poor body esteem on diet, self‐esteem and overall health. Research has examined adolescent body esteem; however, little is known about factors influencing body esteem of younger children. This study tested the moderating effect of child weight status on the links of body esteem of 1st grade children to parental feeding practices (e. g., monitoring), parental concern about child’s weight, and parental weight. Child Body Esteem Scale (BES) and anthropometrics were completed in individual interviews. Parents completed Child Feeding Questionnaire (feeding practices, concern, self‐assessed parental weight). A total of 410 mother/child dyads (202 girls) participated. Percent of children classified as overweight (BMI‐for‐age 蠅85th) vs. normal weight was: girls‐29% vs. 71%; boys‐27% vs. 73%. Overall regression model was significant for girls (R²=.125, F(10,191)=3.879; p<0.001), but not boys. For girls, weight status moderated the link of monitoring (β= ‐.186, t=‐2.42, p<0.05) and parental concern (β=‐.216, t=‐2.08, p<0.05) to body esteem, with the highest parental concern and monitoring corresponding to the lowest body esteem in overweight girls. Differential impact of feeding practices on body esteem by weight status suggests importance of future body esteem interventions for girls as early as first grade.Grant Funding Source: USDA‐CSREES Grant 05545
The relationship between family sense of coherence, healthy behaviors, and child BMI (808.6)
Authors: Hayes Jenna , Speirs Katherine , Musaad Salma , Wozniak Angela , Sigman‐Grant Madeleine 
Published: June 2021

In a novel approach to considering the childhood obesity epidemic, it is suggested that family sense of coherence (FSOC) may act as a protective factor against the obesogenic environment. Proposed by Antonovsky in his theory of salutogenesis, FSOC is a perceptual orientation that facilitates adaptation to and coping with life stressors. A family with a high FSOC has a stronger motivational basis for utilizing their resources to promote health. An individual’s sense of coherence has been positively associated with healthy food choices and overall health. To date, few have examined the relationship between FSOC and health outcomes. To address this gap in the literature, the relationships between FSOC and healthy behaviors (e.g. fruit and vegetable consumption and physical activity) as well as child BMI were examined using interview data from a sample consisting of 432 low‐income mothers and their 3‐5 year old children. FSOC was significantly related to healthy behaviors such that families with a higher sense of coherence practiced more healthy behaviors. FSOC and child BMI were not related. The finding that FSOC may act as a protective factor through its influence on healthy behaviors can inform childhood obesity prevention efforts. Though child weight status may not be affected at the preschool age, the increase of healthy behaviors may affect child BMI at a later age.Grant Funding Source: Supported by USDA NIFA Grant/Award #: 2010‐85215‐20662
Dietary supplemental use in Korean adults: data from the 5th Korea National Health and Nutrition Examination Survey(KNHANES V), 2010‐2012 (809.5)
Authors: Lee Yun Jeong , Kang Minji , Cho Yunok , Yun Sung Ha , Oh Kyungwon , Lee Jung Eun , Song YoonJu 
Published: June 2021

This study aimed to develop a dietary supplemental database and to evaluate the contributions of dietary supplemental use to total nutrient intakes in a Korean adult population of KNHANES V (2010‐2012). Information on dietary supplemental use was obtained from a total of 3,850 men and women aged 19 or more years through face‐to‐face interview. We developed a dietary supplemental database and classified into either pharmaceutical products or functional food products based on product name and manufacturing company. Among all frequencies reported on dietary supplements, 23.7% of frequencies were retrieved from pharmaceutical products and 76.3% from functional food products.The most commonly used dietary supplements were vitamin or mineral supplements (57.2%). Among non‐vitamin/mineral supplements, ginseng and omega 3 were most commonly used in Korean adults. When we calculated the contributions of dietary supplemental use to total nutrient intakes, we found that those who used vitamin or mineral supplements had 0.5 to 9 times higher vitamin or mineral intakes compared to those from foods only. In conclusion, because of the common uses of dietary supplements in Korean, it is needed to evaluate adequate intake or potentially excessive intake of nutrients from supplements and its effects on health outcomes.Grant Funding Source: Supported by KCDC(2013E3501300)
Immune response influences in an adolescent cohort examined for the pathogenesis of mood disorders (811.26)
Authors: Aldoori Ayat , Algorta Guillermo , Ziouzenkova Ouliana , Gracious Barbara 
Published: June 2021

We investigated immune response and oxidative stress in adolescent subjects with varying severities of mood disorders. To assess immune response we measured the profile of immunomodulatory vitamin D, cytokines monocyte chemotactic protein‐1 (MCP‐1) and interleukin‐6 (IL‐6), as well as their transcriptional regulator nuclear factor‐kappa B (NF‐kB) in patients’ serum. Although the markers of inflammation were not significantly different between groups, there appears to be an inverse trend (mean non‐mood 136.19±37.61 versus severe mood 110.76±43.77, p=0.20) between severity of mood disorders and NF‐kB activation. Similarly, MCP‐1 and IL‐6 levels tend to decrease with increasing severity of mood disorder. Obesity measured by BMI significantly correlates with serum IL‐6 levels (r=0.29, p=0.02). There was a significant inverse correlation between BMI of subjects and vitamin D (r=‐0.40, p=0.001) and a significant inverse correlation between vitamin D and IL‐6 (r=‐0.28, p=0.03). There was a significant correlation between vitamin D level and anti‐oxLDL (r=0.28, p=0.03) and a positive trend between vitamin D and MCP‐1 levels (r=0.23, p=0.07). Our findings suggest that lower vitamin D levels are associated with higher BMI in mood disorder patients. The elevated BMI is associated with higher adipose tissue mass producing IL‐6 which possibly leads to higher serum IL‐6 levels. This response appears to be counteracted by vitamin D. Groups affected by varying severity of mood disorders have no pronounced difference in immune response measured by several factors, although groups with the same diagnosis may yield a different a result.Grant Funding Source: Supported by NIMH
Many college students desire food‐related rewards for short‐term goals to improve diet and physical activity (811.27)
Authors: Cope Hannah , Wong Siew Sun 
Published: June 2021

Objective: To assess the desired rewards of college students for short‐term goals to improve diet or physical activity (PA). Methods: College students (n=286) enrolled in a required 10‐week health course at Oregon State University volunteered to participate in the Better Eating Starts Today (B.E.S.T) Project in Corvallis, Oregon. Students completed a required assignment that focused on behavioral changes related to healthier eating or increasing PA. Students set three goals with the following format: specific, measurable, attainable, realistic and timely (S.M.A.R.T). Rewards were divided into those associated with fitness or dietary goals then further divided into 1 of 7 subcategories. Results: Among 858 rewards, 810 were used in this analysis; 48 were excluded because the goal was non‐dietary/PA related or missing data. Sixty‐four percent of the rewards were associated with fitness goals, 36% with dietary goals. Food‐related rewards were most frequent in fitness and dietary goals, 33% and 54%, respectively. Entertainment‐related rewards followed with 19% for fitness goals and 23% for dietary goals. Conclusion: Regardless of short‐term goal, the most desired reward is food‐related. Therefore, educators may need to pay more attention to teach college students to incentivize themselves for health behavior change in creative yet sensible and balanced ways.Grant Funding Source: PacificSource Healthcare
Evidence of developmental continuity from birth to one year: sleep, temperament, problem‐solving, and recognition memory (813.4)
Authors: Judge Michelle , Chang Luye , Lammi‐Keefe Carol 
Published: June 2021

OBJECTIVE: Enhance the understanding of the associations between various cognitive tests and test timing used in nutrition research during the first year of life. METHODS: A longitudinal, prospective, correlational design was used to evaluate if a developmental continuity exists in a single cohort of infants. Infants of women (18‐35 y) with no pregnancy complications (n=27) were assessed using: Sleep (first 48 hours of life); temperament (ICQ 6 & RITQ, 12 mos.); problem‐solving (PS) (Willatts Infant Planning Test, 9 & 12 mos.); and Fagan Test of Infant Intelligence (FTII, 6 & 9 mos.). RESULTS: Using Spearmen correlation, there were significant positive correlations between: transitional sleep and unpredictable (r=.455, P=.017), unadaptable (r= .420, P=.026) and dull (r= .416, P=.028), ICQ domains at 6 mos. and FTII scaled novelty score at 6 mos. (r= ‐.512, P=.008). Quiet sleep was positively associated with the RITQ approachability domain at 12 mos. (r= .662, P=.005). Arousals in active sleep were negatively correlated with PS intentional solutions at 9 mos. (r= ‐528, P=.016). CONCLUSIONS: Early sleep characteristics are reflective of later infant development in the first year. These data support the notion that continuity exists between early and later developmental measures throughout the first year.Grant Funding Source: Supported in part by USDA IFAFS, Nestec S.A., Switzerland, the Hatch Act, National Fisheries Inst.
Amino acid specific natural 15N abundances of hair can change during pregnancy and nutritional stress (820.12)
Authors: Petzke Klaus , Fuller Benjamin 
Published: June 2021

Natural 15N and 13C stable isotope (SI) abundances of hair can be used as nutritional and physiological biomarkers. Changes of amino acid (AA) specific SI‐signatures during pregnancy and its complications are unknown. We suspected influences due to strongly increased requirements for indispensable AA during pregnancy and influences by pathophysiological complications such as nausea and vomiting. 15N and 13C SI‐abundances were measured by GC/C/IRMS using N‐pivaloyl‐i‐propyl esters of AA of protein hydrolysates of hair samples collected after birth. The SI‐patterns differed in a characteristic way between individual AA. The δ‐values of threonine were depleted in 15N (‐9.5 ± 1.1 ‰ vs. AIR‐N2) and those of glycine were enriched in 13C (‐5.7 ± 5.2 ‰ vs. V‐PDB) in comparison to other AA detected. Plots of δ15N‐ against 13C δ‐values of individual AA confirmed the distinct differences between indispensable and dispensable AA. The δ13C‐values did not change significantly during pregnancy. The δ15N‐values of specific AA can change by more than 1 ‰ during pregnancy. The δ15N values of dispensable proline can rise and those of indispensable threonine can decrease. Significantly reduced or enhanced δ15N‐values of AA carrying dispensable N such as leucine or proline can indicate positive or negative N‐balances respectively. The results can have important implications in nutrition, forensics, archaeology, and ecology.Grant Funding Source: Supported by Deutsche Forschungsgemeinschaft, Bonn, Germany (grant numbers: Pe 643/6‐1)
Effects of high and normocaloric diets on serum and thymus fatty acid profile: study on experimental model (821.3)
Authors: Perris Paula , Silva Carolina , Fernandez Inés , Mambrin Cecilia , Slobodianik Nora , Feliu María 
Published: June 2021

Objetive: To study the impact of high and normocaloric diets on serum and thymus´ fatty acid profile in growing rats. Weaning Wistar rats were fed during 10 days with 40% and 15% Kcal dietary fat provided by olive oil (groups O40; O15) high oleic oil (groups HO40; HO15) complete in all nutrients. Control group(C) received normocaloric diet. ω6/ω3 diets ratio: O=49,6 HO=86; C=9; Serum and thymus´ fatty acid profiles were determined by gas chromatography. Results (area% ±SD) ANOVA‐DUNNETT test was perform (*p<0.01):

SERUM
O40
HO40
O15
HO15
C

Oleic
22.0±5.1 *
33.0±4.8 *
19,40±3,4*
26,88±3,1 *
10,6 ±2,0

Linoleic
11.8±2.8 *
8.9±1.0 *
12,44±1,8 *
7,89±1.4 *
19.0±3,5

Linolenic
0.5±0.2*
0.3±0.1*
0.34±0,06*
0.22±0.03*
1.2±0.3

THYMUS

Oleic
25,4±6,7 *
39.1±10,9 *
21,5±5,9
23,7±6,4
18,2 ±3,2

Linoleic
7,2±0,7 *
4,4±0,6 *
5,9±0,6 *
3,1±0,9 *
9,5±0,7

Linolenic
0.3±0.09*
0.2±0.03*
0.3±0.02*
0.2±0.06*
0,6±0,1

All experimental groups showed higher serum oleic and lower Linoleic and Linolenic acid levels compared with C. On thymus only 40% groups showed higher serum oleic acid, but all groups had lower Linoleic and Linolenic acids than C. Serum fatty acid levels changed in response to variations in fat%, ω6/ω3 diets ratio and the sources of dietary lipids. These facts would exacerbate the route of the ω9 family and decreases essential fatty acids.Grant Funding Source: Supported by UBACyT:20020120200068
Gut microbes regulate hepatic nuclear receptor expression (821.7)
Authors: Martinez Kristina , Leone Vanessa , Huang Edmond , Lukens John , Reardon‐Alulis Catherine , Chang Eugene 
Published: June 2021

Gut microbes profoundly affect host health and physiology, exemplified by the finding that germ free (GF) mice are resistant to diet‐induced obesity. However, mechanisms behind this phenomenon are incompletely understood. Preliminary microarray results revealed that nuclear receptors (NR), constitutive androstane receptor (CAR) and peroxisome proliferator‐activated receptor (PPAR)α, were induced in GF versus conventional (Conv) mice. Due to their role in energy and lipid metabolism, we hypothesized that elevated levels of CAR and PPARα protect GF mice from diet‐induced weight gain. To establish proof of principle, Conv and GF mice were fed a lowfat (LF) or highfat (HF) diet for 4 weeks. Body weight, serum lipid levels, and hepatic NR and target gene expression were measured. As expected, HF‐fed Conv mice gained more weight than LF‐fed mice, while GF mice remained resistant to weight gain. GF mice displayed lower levels of portal triglycerides (TG) and free fatty acids versus Conv mice, suggesting increased uptake by peripheral tissues. Additionally, the HF diet reduced portal TG levels, but to a lesser extent in GF mice. PPARα target genes, cytochrome p450 (cyp)4a10 and cluster of differentiation 36/fatty acid translocase CD36/FAT, as well as CAR and its target cyp2b10 were highly expressed in GF mice compared to Conv mice. Interestingly, the HF diet significantly induced CD36 and CAR expression compared to the LF diet in Conv, but not in GF mice. Collectively, these findings suggest a relationship between the gut microbiota and hepatic NR expression. However, further studies are needed to better elucidate these interactions, functional consequences, and the mechanisms through which they occur.Grant Funding Source: Supported by NIH 3T32DK007074‐39S1