bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Pseudo‐Lagrangian Transformation to Study a Chlorophyll‐a Patch in the Ría de Vigo (NW Iberian Peninsula)
Authors: Villamaña M. , Franks P. J. S. , Fernández Castro B. , Gilcoto M. , Marañón E. , Mouriño‐Carballido B. 
Published: October 2021

AbstractBecause of the difficulties in tracking a water mass over time and conducting synoptic surveys over large spatial scales, measurements of biological variables in the ocean are often restricted to isolated Eulerian observations. Here a proof‐of‐concept of a pseudo‐Lagrangian transformation was applied to a time series of chlorophyll‐a profiles acquired at a single fixed station to diagnose the shape of a phytoplankton patch, and its physical‐biological rates of transformation. During August 2013, a 27‐hr time series of observations of horizontal currents, hydrographic properties, turbulent mixing and chlorophyll‐a was acquired at a central station of the Ría de Vigo (NW Iberian Peninsula). A chlorophyll‐a patch, tilted upward toward the inner part of the Ría, was observed moving back and forth past the sampling station. Its shape and position during the sampling period were modulated by the semi‐diurnal (M2) tidal currents and the vertically sheared upwelling circulation. The pseudo‐Lagrangian transformation allowed estimation of chlorophyll‐a net rates of change. These rates were positive between 12 and 14 m depth, and negative elsewhere, with a mean value of −0.001 ± 0.449 days−1 within the patch maximum. After accounting for the effects of diffusion and sinking, the mean net biological production rate in the upper 15 m (0.53 ± 0.25 days−1) was comparable with in vitro estimates of the balance between phytoplankton growth and mortality obtained from dilution experiments carried out under similar conditions (0.2 ± 0.7 days−1). This pseudo‐Lagrangian transformation is complementary to traditional analyses for the quantification of ecological processes and biogeochemical budgets.
Inducible Prmt1 ablation in adult vascular smooth muscle leads to contractile dysfunction and aortic dissection
Authors: Pyun Jung-Hoon , Ahn Byeong-Yun , Vuong Tuan Anh , Kim Su Woo , Jo Yunju , Jeon Jaehyung , Baek Seung Ho , Kim Jaewon , Park Sungsu , Bae Gyu-Un , Choi Jun-Hyuk , Kim Jae-Ryong , Ryu Dongryeol , Lee Sang-Jin , Kang Jong-Sun 
Published: October 2021

AbstractVascular smooth muscle cells (VSMCs) have remarkable plasticity in response to diverse environmental cues. Although these cells are versatile, chronic stress can trigger VSMC dysfunction, which ultimately leads to vascular diseases such as aortic aneurysm and atherosclerosis. Protein arginine methyltransferase 1 (Prmt1) is a major enzyme catalyzing asymmetric arginine dimethylation of proteins that are sources of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase. Although a potential role of Prmt1 in vascular pathogenesis has been proposed, its role in vascular function has yet to be clarified. Here, we investigated the role and underlying mechanism of Prmt1 in vascular smooth muscle contractility and function. The expression of PRMT1 and contractile-related genes was significantly decreased in the aortas of elderly humans and patients with aortic aneurysms. Mice with VSMC-specific Prmt1 ablation (smKO) exhibited partial lethality, low blood pressure and aortic dilation. The Prmt1-ablated aortas showed aortic dissection with elastic fiber degeneration and cell death. Ex vivo and in vitro analyses indicated that Prmt1 ablation significantly decreased the contractility of the aorta and traction forces of VSMCs. Prmt1 ablation downregulated the expression of contractile genes such as myocardin while upregulating the expression of synthetic genes, thus causing the contractile to synthetic phenotypic switch of VSMCs. In addition, mechanistic studies demonstrated that Prmt1 directly regulates myocardin gene activation by modulating epigenetic histone modifications in the myocardin promoter region. Thus, our study demonstrates that VSMC Prmt1 is essential for vascular homeostasis and that its ablation causes aortic dilation/dissection through impaired myocardin expression.
Kleptoplast distribution, photosynthetic efficiency and sequestration mechanisms in intertidal benthic foraminifera
Authors: Jesus Bruno , Jauffrais Thierry , Trampe Erik C L , Goessling Johannes W , Lekieffre Charlotte , Meibom Anders , Kühl Michael , Geslin Emmanuelle 
Published: October 2021

Abstract
Foraminifera are ubiquitously distributed in marine habitats, playing a major role in marine sediment carbon sequestration and the nitrogen cycle. They exhibit a wide diversity of feeding and behavioural strategies (heterotrophy, autotrophy and mixotrophy), including species with the ability of sequestering intact functional chloroplasts from their microalgal food source (kleptoplastidy), resulting in a mixotrophic lifestyle. The mechanisms by which kleptoplasts are integrated and kept functional inside foraminiferal cytosol are poorly known. In our study, we investigated relationships between feeding strategies, kleptoplast spatial distribution and photosynthetic functionality in two shallow-water benthic foraminifera (Haynesina germanica and Elphidium williamsoni), both species feeding on benthic diatoms. We used a combination of observations of foraminiferal feeding behaviour, test morphology, cytological TEM-based observations and HPLC pigment analysis, with non-destructive, single-cell level imaging of kleptoplast spatial distribution and PSII quantum efficiency. The two species showed different feeding strategies, with H. germanica removing diatom content at the foraminifer’s apertural region and E. williamsoni on the dorsal site. All E. williamsoni parameters showed that this species has higher autotrophic capacity albeit both feeding on benthic diatoms. This might represent two different stages in the evolutionary process of establishing a permanent symbiotic relationship, or may reflect different trophic strategies.
Rapid diagnosis of COVID-19 using FT-IR ATR spectroscopy and machine learning
Authors: Nogueira Marcelo Saito , Leal Leonardo Barbosa , Marcarini Wena Dantas , Pimentel Raquel Lemos , Muller Matheus , Vassallo Paula Frizera , Campos Luciene Cristina Gastalho , dos Santos Leonardo , Luiz Wilson Barros , Mill José Geraldo , Barauna Valerio Garrone , de Carvalho Luis Felipe das Chagas e Silva 
Published: October 2021

AbstractEarly diagnosis of COVID-19 in suspected patients is essential for contagion control and damage reduction strategies. We investigated the applicability of attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy associated with machine learning in oropharyngeal swab suspension fluid to predict COVID-19 positive samples. The study included samples of 243 patients from two Brazilian States. Samples were transported by using different viral transport mediums (liquid 1 or 2). Clinical COVID-19 diagnosis was performed by the RT-PCR. We built a classification model based on partial least squares (PLS) associated with cosine k-nearest neighbours (KNN). Our analysis led to 84% and 87% sensitivity, 66% and 64% specificity, and 76.9% and 78.4% accuracy for samples of liquids 1 and 2, respectively. Based on this proof-of-concept study, we believe this method could offer a simple, label-free, cost-effective solution for high-throughput screening of suspect patients for COVID-19 in health care centres and emergency departments.
Synchronous imaging of pelvic geometry and muscle morphometry: a pilot study of pelvic retroversion using upright MRI
Authors: Shaikh Noor , Zhang Honglin , Brown Stephen H. M. , Lari Hamza , Lasry Oliver , Street John , Wilson David R. , Oxland Thomas 
Published: October 2021

AbstractThis study investigated feasibility of imaging lumbopelvic musculature and geometry in tandem using upright magnetic resonance imaging (MRI) in asymptomatic adults, and explored the effect of pelvic retroversion on lumbopelvic musculature and geometry. Six asymptomatic volunteers were imaged (0.5 T upright MRI) in 4 postures: standing, standing pelvic retroversion, standing 30° flexion, and supine. Measures included muscle morphometry [cross-sectional area (CSA), circularity, radius, and angle] of the gluteus and iliopsoas, and pelvic geometry [pelvic tilt (PT), pelvic incidence (PI), sacral slope (SS), L3–S1 lumbar lordosis (LL)] L3-coccyx. With four volunteers repeating postures, and three raters assessing repeatability, there was generally good repeatability [ICC(3,1) 0.80–0.97]. Retroversion had level dependent effects on muscle measures, for example gluteus CSA and circularity increased (up to 22%). Retroversion increased PT, decreased SS, and decreased L3–S1 LL, but did not affect PI. Gluteus CSA and circularity also had level-specific correlations with PT, SS, and L3–S1 LL. Overall, upright MRI of the lumbopelvic musculature is feasible with good reproducibility, and the morphometry of the involved muscles significantly changes with posture. This finding has the potential to be used for clinical consideration in designing and performing future studies with greater number of healthy subjects and patients.
Assessing the cascading impacts of natural disasters in a multi-layer behavioral network framework
Authors: Naqvi Asjad , Monasterolo Irene 
Published: October 2021

AbstractNatural disasters negatively impact regions and exacerbate socioeconomic vulnerabilities. While the direct impacts of natural disasters are well understood, the channels through which these shocks spread to non-affected regions, still represents an open research question. In this paper we propose modelling socioeconomic systems as spatially-explicit, multi-layer behavioral networks, where the interplay of supply-side production, and demand-side consumption decisions, can help us understand how climate shocks cascade. We apply this modelling framework to analyze the spatial-temporal evolution of vulnerability following a negative food-production shock in one part of an agriculture-dependent economy. Simulation results show that vulnerability is cyclical, and its distribution critically depends on the network density and distance from the epicenter of the shock. We also introduce a new multi-layer measure, the Vulnerability Rank (VRank), which synthesizes various location-level risks into a single index. This framework can help design policies, aimed to better understand, effectively respond, and build resilience to natural disasters. This is particularly important for poorer regions, where response time is critical and financial resources are limited.
Molecular testing and analysis of disease spreading during the emergence of COVID-19 in Macaé, the Brazilian National Capital of Oil
Authors: Feitosa Natália Martins , da Costa Rodrigues Bruno , Petry Ana Cristina , Nocchi Keity Jaqueline Chagas Vilela , de Moraes Brindeiro Rodrigo , Zilberberg Carla , Monteiro-de-Barros Cintia , Mury Flavia Borges , de Souza-Menezes Jackson , Nepomuceno-Silva José Luciano , da Silva Manuela Leal , de Medeiros Marcio José , de Souza Gestinari Raquel , da Silva de Alvarenga Alessandra , Pozzobon Allan Pierre Bonetti , Silva Carina Azevedo Oliveira , das Graças dos Santos Daniele , Silvestre Diego Henrique , de Sousa Graziele Fonseca , de Almeida Janimayri Forastieri , da Silva Jhenifer Nascimento , Brandão Layza Mendes , de Oliveira Drummond Leandro , Neto Lupis Ribeiro Gomes , de Mello Carpes Raphael , dos Santos Renata Coutinho , Portal Taynan Motta , Tanuri Amilcar , Nunes-da-Fonseca Rodrigo 
Published: October 2021

AbstractThe Brazilian strategy to overcome the spread of COVID-19 has been particularly criticized due to the lack of a national coordinating effort and an appropriate testing program. Here, a successful approach to control the spread of COVID-19 transmission is described by the engagement of public (university and governance) and private sectors (hospitals and oil companies) in Macaé, state of Rio de Janeiro, Brazil, a city known as the National Oil Capital. In 2020 between the 17th and 38th epidemiological week, over two percent of the 206,728 citizens were subjected to symptom analysis and RT-qPCR testing by the Federal University of Rio de Janeiro, with positive individuals being notified up to 48 h after swab collection. Geocodification and spatial cluster analysis were used to limit COVID-19 spreading in Macaé. Within the first semester after the outbreak of COVID-19 in Brazil, Macaé recorded 1.8% of fatalities associated with COVID-19 up to the 38th epidemiological week, which was at least five times lower than the state capital (10.6%). Overall, considering the successful experience of this joint effort of private and public engagement in Macaé, our data suggest that the development of a similar strategy countrywise could have contributed to a better control of the COVID-19 spread in Brazil. Quarantine decree by the local administration, comprehensive molecular testing coupled to scientific analysis of COVID-19 spreading, prevented the catastrophic consequences of the pandemic as seen in other populous cities within the state of Rio de Janeiro and elsewhere in Brazil.
The mitochondrial signaling peptide MOTS-c improves myocardial performance during exercise training in rats
Authors: Yuan Jinghan , Wang Manda , Pan Yanrong , Liang Min , Fu Yu , Duan Yimei , Tang Mi , Laher Ismail , Li Shunchang 
Published: October 2021

AbstractCardiac remodeling is a physiological adaptation to aerobic exercise and which is characterized by increases in ventricular volume and the number of cardiomyocytes. The mitochondrial derived peptide MOTS-c functions as an important regulator in physical capacity and performance. Exercise elevates levels of endogenous MOTS-c in circulation and in myocardium, while MOTS-c can significantly enhance exercise capacity. However, the effects of aerobic exercise combined with MOTS-c on cardiac structure and function are unclear. We used pressure–volume conductance catheter technique to examine cardiac function in exercised rats with and without treatment with MOTS-c. Surprisingly, MOTS-c improved myocardial mechanical efficiency, enhanced cardiac systolic function, and had a tendency to improve the diastolic function. The findings suggest that using exercise supplements could be used to modulate the cardiovascular benefits of athletic training.
Immunometabolic factors in adolescent chronic disease are associated with Th1 skewing of invariant Natural Killer T cells
Authors: Ververs Francesca A. , Engelen Suzanne E. , Nuboer Roos , Vastert Bas , van der Ent Cornelis K. , van’t Land Belinda , Garssen Johan , Monaco Claudia , Boes Marianne , Schipper Henk S. 
Published: October 2021

AbstractInvariant Natural Killer T (iNKT) cells respond to the ligation of lipid antigen-CD1d complexes via their T-cell receptor and are implicated in various immunometabolic diseases. We considered that immunometabolic factors might affect iNKT cell function. To this end, we investigated iNKT cell phenotype and function in a cohort of adolescents with chronic disease and immunometabolic abnormalities. We analyzed peripheral blood iNKT cells of adolescents with cystic fibrosis (CF, n = 24), corrected coarctation of the aorta (CoA, n = 25), juvenile idiopathic arthritis (JIA, n = 20), obesity (OB, n = 20), and corrected atrial septal defect (ASD, n = 25) as controls. To study transcriptional differences, we performed RNA sequencing on a subset of obese patients and controls. Finally, we performed standardized co-culture experiments using patient plasma, to investigate the effect of plasma factors on iNKT cell function. We found comparable iNKT cell numbers across patient groups, except for reduced iNKT cell numbers in JIA patients. Upon ex-vivo activation, we observed enhanced IFN-γ/IL-4 cytokine ratios in iNKT cells of obese adolescents versus controls. The Th1-skewed iNKT cell cytokine profile of obese adolescents was not explained by a distinct transcriptional profile of the iNKT cells. Co-culture experiments with patient plasma revealed that across all patient groups, obesity-associated plasma factors including LDL-cholesterol, leptin, and fatty-acid binding protein 4 (FABP4) coincided with higher IFN-γ production, whereas high HDL-cholesterol and insulin sensitivity (QUICKI) coincided with higher IL-4 production. LDL and HDL supplementation in co-culture studies confirmed the effects of lipoproteins on iNKT cell cytokine production. These results suggest that circulating immunometabolic factors such as lipoproteins may be involved in Th1 skewing of the iNKT cell cytokine response in immunometabolic disease.
Anthrahydroquinone-2-6-disulfonate is a novel, powerful antidote for paraquat poisoning
Authors: Qian Jin , Wu Chun-Yuan , Wu Dong-Ming , Li Li-Hua , Li Qi , Deng Tang , Huang Qi-Feng , Xu Shuang-Qin , Wang Hang-Fei , Wu Xin-Xin , Cheng Zi-Yi , Lv Chuan-Zhu , Liu Xiao-Ran 
Published: October 2021

AbstractParaquat (PQ) is a widely used fast-acting pyridine herbicide. Accidental ingestion or self-administration via various routes can cause severe organ damage. Currently, no effective antidote is available commercially, and the mortality rate of poisoned patients is exceptionally high. Here, the efficacy of anthrahydroquinone-2-6-disulfonate (AH2QDS) was observed in treating PQ poisoning by constructing in vivo and ex vivo models. We then explored the detoxification mechanism of AH2QDS. We demonstrated that, in a rat model, the PQ concentration in the PQ + AH2QDS group significantly decreased compared to the PQ only group. Additionally, AH2QDS protected the mitochondria of rats and A549 cells and decreased oxidative stress damage, thus improving animal survival and cell viability. Finally, the differentially expressed genes were analysed in the PQ + AH2QDS group and the PQ group by NextGen sequencing, and we verified that Nrf2’s expression in the PQ + AH2QDS group was significantly higher than that in the PQ group. Our work identified that AH2QDS can detoxify PQ by reducing PQ uptake and protecting mitochondria while enhancing the body's antioxidant activity.
Pharmacoethnicity of FOLFIRINOX versus gemcitabine plus nab-paclitaxel in metastatic pancreatic cancer: a systematic review and meta-analysis
Authors: Lee Yoon Suk , Lee Jong-chan , Kim Jae-Hyeong , Kim Jaihwan , Hwang Jin-Hyeok 
Published: October 2021

AbstractTreatment outcomes between FOLFIRINOX (5-fluorouracil, leucovorin, irinotecan, and oxaliplatin) and GNP (gemcitabine with albumin-bound paclitaxel) as first-line chemotherapy regimens for metastatic pancreatic cancer (PC) were assessed according to ethnic groups categorized as Western or Asian subgroups. PubMed, EMBASE, and Cochrane library were searched. Thirteen studies were eligible in this meta-analysis. Overall survival was not significantly different between FOLFIRINOX and GNP (HR 1.00, 95% CI 0.83–1.20, P = 0.990). However, the Western subgroup showed a higher survival benefit for FOLFIRINOX over GNP (HR 0.84, 95% CI 0.74–0.95, P = 0.006) whereas the Asian subgroup showed the survival benefit for GNP over FOLFIRINOX (HR 1.29, 95% CI 1.03–1.60, P = 0.030). Progression free survival was not significantly different between the two regimens in the Western subgroup (HR 1.01, 95% CI 0.84–1.20, P = 0.950) and the Asian subgroup (HR 1.13, 95% CI 0.97–1.33, P = 0.110). Occurrence of febrile neutropenia was significantly higher in FOLFIRINOX at both ethnic subgroups; however, that of peripheral neuropathy was significantly higher only in GNP of the Asian subgroup. Therefore, pharmacoethnicity might be a factor worth considering when deciding on a frontline chemotherapeutic regimen although the overall survival was not significantly different between FOLFIRINOX and GNP for metastatic PCs.
Species richness and β-diversity patterns of macrolichens along elevation gradients across the Himalayan Arc
Authors: Nanda Subzar Ahmad , Haq Manzoor-ul , Singh S. P. , Reshi Zafar A. , Rawal Ranbeer S. , Kumar Devendra , Bisht Kapil , Upadhyay Shashi , Upreti D. K. , Pandey Aseesh 
Published: October 2021

AbstractUnderstanding the species richness and β-diversity patterns along elevation gradients can aid in formulating effective conservation strategies particularly in areas where local anthropogenic stresses and climate change are quite significant as in the Himalaya. Thus, we studied macrolichen richness and β-diversity along elevational gradients at three sites, namely Kashmir (2200 to 3800 m a.m.s.l), Uttarakhand (2000–3700 m a.m.s.l) and Sikkim (1700 to 4000 m a.m.s.l) which cover much of the Indian Himalayan Arc. In all, 245 macrolichen species belonging to 77 genera and 26 families were collected from the three sites. Only 11 species, 20 genera and 11 families were common among the three transects. Despite the differences in species composition, the dominant functional groups in the three sites were the same: foliose, fruticose and corticolous forms. The hump-shaped elevation pattern in species richness was exhibited by most of the lichen groups, though an inverse hump-shaped pattern was also observed in certain cases. β-diversity (βsor) based on all pairs of comparisons along an elevation gradient varied from 0.48 to 0.58 in Kashmir, 0.03 to 0.63 in Uttarakhand and 0.46 to 0.77 in Sikkim. The contribution of turnover to β-diversity was more than nestedness at all the three transects. Along elevation β-diversity and its components of turnover and nestedness varied significantly with elevation. While species turnover increased significantly along the elevation in all the three transects, nestedness decreased significantly in Kashmir and Sikkim transects but increased significantly in the Uttarakhand transect. Except for the Kashmir Himalayan elevation transect, stepwise β-diversity and its components of turnover and nestedness did not vary significantly with elevation. The present study, the first of its kind in the Himalayan region, clearly brings out that macrolichen species richness, β-diversity, and its components of turnover and nestedness vary along the elevation gradients across the Himalayan Arc. It also highlights that contribution of turnover to β-diversity is higher in comparison to nestedness at all the three transects. The variations in species richness and diversity along elevation gradients underpin the importance of considering elevational gradients in planning conservation strategies.