bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the potential of wearable bioimpedance for longitudinal fluid monitoring in end-stage kidney disease
Authors: Lindeboom Lucas , Lee Seulki , Wieringa Fokko , Groenendaal Willemijn , Basile Carlo , van der Sande Frank , Kooman Jeroen 
Published: February 2021

Abstract
Bioimpedance spectroscopy (BIS) has proven to be a promising non-invasive technique for fluid monitoring in haemodialysis (HD) patients. While current BIS-based monitoring of pre- and post-dialysis fluid status utilizes benchtop devices, designed for intramural use, advancements in micro-electronics have enabled the development of wearable bioimpedance systems. Wearable systems meanwhile can offer a similar frequency range for current injection as commercially available benchtop devices. This opens opportunities for unobtrusive longitudinal fluid status monitoring, including transcellular fluid shifts, with the ultimate goal of improving fluid management, thereby lowering mortality and improving quality of life for HD patients. Ultra-miniaturized wearable devices can also offer simultaneous acquisition of multiple other parameters, including haemodynamic parameters. Combination of wearable BIS and additional longitudinal multiparametric data may aid in the prevention of both haemodynamic instability as well as fluid overload. The opportunity to also acquire data during interdialytic periods using wearable devices likely will give novel pathophysiological insights and the development of smart (predicting) algorithms could contribute to personalizing dialysis schemes and ultimately to autonomous (nocturnal) home dialysis. This review provides an overview of current research regarding wearable bioimpedance, with special attention to applications in end-stage kidney disease patients. Furthermore, we present an outlook on the future use of wearable bioimpedance within dialysis practice.
The Medicago truncatula Sugar Transport Protein 13 and Its Lr67res-Like Variant Confer Powdery Mildew Resistance in Legumes via Defense Modulation
Authors: Gupta Megha , Dubey Shubham , Jain Deepti , Chandran Divya 
Published: February 2021

Abstract
Obligate biotrophic pathogens like the pea powdery mildew© (PM) Erysiphe pisi establish long-term feeding relationships with their host, during which they siphon sugars from host cells through haustoria. Plants in turn deploy sugar transporters to restrict carbon allocation toward pathogens, as a defense mechanism. Studies in Arabidopsis have shown that sugar transport protein 13 (STP13), a proton-hexose symporter involved in apoplasmic hexose retrieval, contributes to bacterial and necrotrophic fungal resistance by limiting sugar flux toward these pathogens. By contrast, expression of Lr67res,a transport-deficient wheat STP13 variant harboring two amino acid substitutions (G144R and V387L), conferred resistance against biotrophic fungi in wheat and barley, indicating its broad applicability in disease management. Here, we investigated the role of STP13 and STP13G144R in legume–PM interactions. We show that Medicago truncatula STP13.1 is a proton-hexose symporter involved in basal resistance against PM and indirectly show that Lr67res-mediated PM resistance, so far reported only in monocots, is transferable to legumes. Among the 30 MtSTPs, STP13.1 exhibited the highest fold induction in PM-challenged leaves and was also responsive to chitosan, ABA and sugar treatment. Functional assays in yeast showed that introduction of the G144R mutation but not V388L abolished MtSTP13.1’s hexose uptake ability. Virus-induced gene silencing of MtSTP13 repressed pathogenesis-related (PR) gene expression and enhanced PM susceptibility in M. truncatula whereas transient overexpression of MtSTP13.1 or MtSTP13.1G144R in pea induced PR and isoflavonoid pathway genes and enhanced PM resistance. We propose a model in which STP13.1-mediated sugar signaling triggers defense responses against PM in legumes.
TOR coordinates nucleotide availability with ribosome biogenesis in plants
Authors: Busche Michael , Scarpin M Regina , Hnasko Robert , Brunkard Jacob O 
Published: February 2021

Abstract
TARGET OF RAPAMYCIN (TOR) is a conserved eukaryotic Ser/Thr protein kinase that coordinates growth and metabolism with nutrient availability. We conducted a medium-throughput functional genetic screen to discover essential genes that promote TOR activity in plants, and identified a critical regulatory enzyme, cytosolic phosphoribosyl pyrophosphate (PRPP) synthetase (PRS4). PRS4 synthesizes cytosolic PRPP, a key upstream metabolite in nucleotide synthesis and salvage pathways. We found that prs4 knockouts are embryo-lethal in Arabidopsis thaliana, and that silencing PRS4 expression in Nicotiana benthamiana causes pleiotropic developmental phenotypes, including dwarfism, aberrant leaf shape, and delayed flowering. Transcriptomic analysis revealed that ribosome biogenesis is among the most strongly repressed processes in prs4 knockdowns. Building on these results, we discovered that TOR activity is inhibited by chemical or genetic disruption of nucleotide biosynthesis, but that this effect can be reversed by supplying plants with nucleobases. Finally, we show that TOR transcriptionally promotes nucleotide biosynthesis to support the demands of ribosomal RNA synthesis. We propose that TOR coordinates ribosome biogenesis with nucleotide availability in plants to maintain metabolic homeostasis and support growth.
Rice and Arabidopsis homologs of yeast CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4 commonly interact with Polycomb complexes but exert divergent regulatory functions
Authors: Zhang Pingxian , Zhu Chunmei , Geng Yuke , Wang Yifan , Yang Ying , Liu Qing , Guo Weijun , Chachar Sadaruddin , Riaz Adeel , Yan Shuangyong , Yang Liwen , Yi Keke , Wu Changyin , Gu Xiaofeng 
Published: February 2021

Abstract
Both genetic and epigenetic information must be transferred from mother to daughter cells during cell division. The mechanisms through which information about chromatin states and epigenetic marks like histone 3 lysine 27 trimethylation (H3K27me3) are transferred have been characterized in animals; these processes are less well understood in plants. Here, based on characterization of a dwarf rice (Oryza sativa) mutant (dwarf-related wd40 protein 1, drw1) deficient for yeast CTF4 (CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4), we discovered that CTF4 orthologs in plants use common cellular machinery yet accomplish divergent functional outcomes. Specifically, drw1 exhibited no flowering-related phenotypes (as in the putatively orthologous Arabidopsis thaliana eol1 mutant), but displayed cell cycle arrest and DNA damage responses. Mechanistically, we demonstrate that DRW1 sustains normal cell cycle progression by modulating the expression of cell cycle inhibitors KIP-RELATED PROTEIN 1 (KRP1) and KRP5, and show that these effects are mediated by DRW1 binding their promoters and increasing H3K27me3 levels. Thus, although CTF4 orthologs ENHANCER OF LHP1 1 (EOL1) in Arabidopsis and DRW1 in rice are both expressed uniquely in dividing cells, commonly interact with several Polycomb complex subunits, and promote H3K27me3 deposition, we now know that their regulatory functions diverged substantially during plant evolution. Moreover, our work experimentally illustrates specific targets of CTF4/EOL1/DRW1, their protein–proteininteraction partners, and their chromatin/epigenetic effects in plants.
Current progress in Striga management
Authors: Jamil Muhammad , Kountche Boubacar A , Al-Babili Salim 
Published: February 2021

Abstract
The Striga, particularly S. he rmonthica, problem has become a major threat to food security, exacerbating hunger and poverty in many African countries. A number of Striga control strategies have been proposed and tested during the past decade, however, further research efforts are still needed to provide sustainable and effective solutions to the Striga problem. In this paper, we provide an update on the recent progress and the approaches used in Striga management, and highlight emerging opportunities for developing new technologies to control this enigmatic parasite.
YSL3-mediated copper distribution is required for fertility, seed size and protein accumulation in Brachypodium
Authors: Sheng Huajin , Jiang Yulin , Rahmati Maryam , Chia Ju-Chen , Dokuchayeva Tatyana , Kavulych Yana , Zavodna Tetiana-Olena , Mendoza Patrick N , Huang Rong , Smieshka Louisa M , Miller Julia , Woll Arthur R , Terek Olga I , Romanyuk Nataliya D , Piñeros Miguel , Zhou Yonghong , Vatamaniuk Olena K 
Published: February 2021

Abstract
Addressing the looming global food security crisis requires the development of high-yielding crops. In agricultural soils, deficiency in the micronutrient copper significantly decreases grain yield in wheat (Triticum aestivum), a globally important crop. In cereals, grain yield is determined by inflorescence architecture, flower fertility, grain size, and weight. Whether copper is involved in these processes, and how it is delivered to the reproductive organs is not well understood. We show that copper deficiency alters not only the grain set but also flower development in both wheat and its recognized model, Brachypodium distachyon. We then show that the Brachypodium yellow stripe-like 3 (YSL3) transporter localizes to the phloem, transports copper in frog (Xenopus laevis) oocytes, and facilitates copper delivery to reproductive organs and grains. Failure to deliver copper, but not iron, zinc, or manganese to these structures in the ysl3 CRISPR-Cas9 mutant results in delayed flowering, altered inflorescence architecture, reduced floret fertility, grain size, weight, and protein accumulation. These defects are rescued by copper supplementation and are complemented by YSL3 cDNA. This knowledge will help to devise sustainable approaches for improving grain yield in regions where soil quality is a major obstacle for crop production. Copper distribution by a phloem-localized transporter is essential for the transition to flowering, inflorescence architecture, floret fertility, size, weight, and protein accumulation in seeds.
Consistency of M-Theory on Non-Orientable Manifolds
Authors: Freed Daniel S , Hopkins Michael J 
Published: February 2021

AbstractWe prove that there is no parity anomaly in M-theory in the low-energy field theory approximation. Our approach is computational. We determine the generators for the 12-dimensional bordism group of pin manifolds with a w1-twisted integer lift of w4; these are the manifolds on which Wick-rotated M-theory exists. The anomaly cancellation comes down to computing a specific η-invariant and cubic form on these manifolds. Of interest beyond this specific problem are our expositions of computational techniques for η-invariants, the algebraic theory of cubic forms, Adams spectral sequence techniques and anomalies for spinor fields and Rarita–Schwinger fields.
Second Moment Of Dirichlet L-Functions, Character Sums Over Subgroups And Upper Bounds On Relative Class Numbers
Authors: Louboutin Stéphane R , Munsch Marc 
Published: February 2021

Abstract
We prove an asymptotic formula for the mean-square average of L-functions associated with subgroups of characters of sufficiently large size. Our proof relies on the study of certain character sums ${\mathcal{A}}(p,d)$ recently introduced by E. Elma, where p ≥ 3 is prime and d ≥ 1 is any odd divisor of p − 1. We obtain an asymptotic formula for ${\mathcal{A}}(p,d),$ which holds true for any odd divisor d of p − 1, thus removing E. Elma’s restrictions on the size of d. This answers a question raised in Elma’s paper. Our proof relies on both estimates on the frequency of large character sums and techniques from the theory of uniform distribution. As an application, in the range $1\leq d\leq\frac{\log p}{3\log\log p}$, we obtain a significant improvement $h_{p,d}^- \leq 2(\frac{(1+o(1))p}{24})^{m/4}$ over the trivial bound $h_{p,d}^- \ll (\frac{dp}{24} )^{m/4}$ on the relative class numbers of the imaginary number fields of conductor $p\equiv 1\mod{2d}$ and degree $m=(p-1)/d$, where d ≥ 1 is odd.
Associations of blood pressure and arterial stiffness with knee cartilage volume in patients with knee osteoarthritis
Authors: Wang Yuanyuan , Meng Tao , Ruan Guangfeng , Zheng Shuang , Zhu Jimin , Cen Han , Antony Benny , Wluka Anita E , Cicuttini Flavia , Winzenberg Tania , Pelletier Jean-Pierre , Martel-Pelletier Johanne , Ding Changhai 
Published: February 2021

AbstractObjectiveTo describe the associations of blood pressure and arterial stiffness with knee cartilage volume in patients with knee OA.MethodsA secondary analysis was performed on the data from participants in a randomized controlled trial that identified the effects of vitamin D supplementation on knee structures and symptoms among patients with symptomatic knee OA. Brachial and central blood pressure, arterial stiffness indicators and knee cartilage volume were measured at baseline and the 2 year follow-up. Associations were assessed using generalized estimating equations.ResultsAmong 231 participants (average age 63.2 years), 48.9% were females. Higher supine systolic and diastolic pressures were significantly associated with lower tibial cartilage volume (systolic: lateral β −6.23, medial β −5.14, total β −11.35 mm3/mmHg; diastolic: lateral β −10.25, medial β −11.29, total β −21.50 mm3/mmHg). Higher supine systolic pressure was associated with lower femoral cartilage volume (lateral β −17.35, total β −28.31 mm3/mmHg). Central systolic pressure and arterial stiffness indicators (including pulse wave velocity, central pulse pressure and peripheral pulse pressure) were largely not associated with knee cartilage volume; however, higher augmentation index was associated with lower tibial and femoral cartilage volume (tibial: medial β −8.24, total β −19.13 mm3/%; femoral: lateral β −23.70, medial β −26.42, total β −50.12 mm3/%).ConclusionsBlood pressure and arterial stiffness are associated with knee cartilage volume at several sites in knee OA patients. This supports that blood pressure and arterial stiffness may involve in the progression of knee OA.
Effect of Music on Outcomes of Birth Asphyxia: A Randomized Controlled Trial
Authors: Konar Mithun Chandra , Islam Kamirul , Sil Archan , Nayek Kaustav , Barik Kanailal 
Published: February 2021

Abstract

Introduction
Birth asphyxia may cause neuro-developmental impairment in the affected newborns especially those who had hypoxic–ischemic encephalopathy. Music therapy has been observed to help in reducing pain and stress in newborns and improve neurodevelopmental outcome.

Objectives
The objective of this study is to determine the effects of music therapy on the outcomes of birth asphyxia.

Methods
A randomized controlled trial involving 3095 newborns born between January 2013 and August 2019 with birth asphyxia was conducted in the neonatal intensive care unit of Burdwan Medical College. They were distributed in two groups—A (received music therapy along with coventional management) and B (only received conventional management), using computer-generated randomization. Pain score was assessed during any painful procedure and the neurodevelopmental outcome was measured at 3rd, 6th, 12th, 18th and 24th months. p &lt; 0.01 was considered statistically significant.

Results
A total of 3095 newborns were included with a mean gestation of 34.3 ± 2.1 weeks and 56.7% of them were male. Mean hospital stay, oxygen dependency, requirement of mechanical ventilation and incidence of apnea were significantly lesser among newborns of group A. Newborns of group B showed a significantly higher mean pain score, whereas newborns in Group A exhibited significantly greater mental and motor neurodevelopmental quotients.

Conclusions
Music therapy was observed to help in reducing hospital stay, oxygen dependency, incidences of apnea, pain during procedures and also resulted in better neurodevelopmental outcome. However, before generalizing the findings, further multi-centric research should be undertaken.
Foot Length for Gestational Age Assessment and Identification of High-Risk Infants: A Hospital-Based Cross-Sectional Study
Authors: Tergestina Mintoo , Chandran Shanu , Kumar Manish , Rebekah Grace , Ross Benjamin J 
Published: February 2021

Abstract
The knowledge of the gestational age of the newborn is essential for management. In the absence of a dating scan, the postnatal assessment scores have drawbacks of being difficult to learn and administer in the community. The measurement of the foot length is easy, reproducible and offers an objective assessment. The objective of this study was to determine the correlation of postnatal (&lt;48 h) foot length measurement (with calipers) with gestational age as determined by antenatal dating ultrasound, create a predictive model for the same and propose foot length measurement cutoffs for &lt;37 and &lt;34 weeks of gestation. Secondary objectives were to assess the correlation between foot length as measured with calipers and that measured with a ruler and a paper footprint. This was a hospital-based cross-sectional study. Among the 520 babies assessed, the correlation of foot length with gestational age was 0.89. Operational cutoffs for the categories of &lt;37 and &lt;34 weeks at a sensitivity of 95% were &lt;70 and &lt;65 mm, respectively. The Pearson’s correlation between foot length as measured by caliper and ruler was 0.95 and between caliper and paper footprint was 0.87. This study correlating foot length and gestational age has the potential to help neonatal care providers make informed management decisions, particularly in resource-limited settings.
Rethinking neglected tropical disease prevalence survey design and analysis: a geospatial paradigm
Authors: Diggle Peter J , Amoah Benjamin , Fronterre Claudio , Giorgi Emanuele , Johnson Olatunji 
Published: February 2021

Abstract
Current methods for the design and analysis of neglected tropical disease prevalence surveys largely rely on classical survey sampling ideas that treat prevalence data from different locations as an independent random sample from the probability distribution induced by a random sampling design. We set out an alternative, explicitly geospatial paradigm that can deliver much more precise estimates of the geospatial variation in prevalence over a country or region of interest. We describe the advantages of this approach under three headings: streamlining, whereby more precise results can be obtained with smaller sample sizes; integrating, whereby a joint analysis of data from two or more diseases can bring further gains in precision; and adapting, whereby the choice of future sampling location is informed by past data.
Phylogeny of Salmonella enterica subspecies arizonae by whole-genome sequencing reveals high incidence of polyphyly and low phase 1 H antigen variability
Authors: Shariat Nikki W. , Timme Ruth E. , Walters Abigail T. 
Published: February 2021

Salmonella enterica

subspecies

arizonae

is frequently associated with animal reservoirs, particularly reptiles, and can cause illness in some mammals, including humans. Using whole-genome sequencing data, core genome phylogenetic analyses were performed using 112

S

.

enterica

subsp.

arizonae

isolates, representing 46 of 102 described serovars. Nearly one-third of these are polyphyletic, including two serovars that appear in four and five distinct evolutionary lineages. Subspecies arizonae has a monophasic H antigen. Among the 46 serovars investigated, only 8 phase 1 H antigens were identified, demonstrating high conservation for this antigen. Prophages and plasmids were found throughout this subspecies, including five novel prophages. Polyphyly was also reflected in prophage content, although some clade-specific enrichment for some phages was observed. IncFII(S) was the most frequent plasmid replicon identified and was found in a quarter of

S. enterica

subsp.

arizonae

genomes.

Salmonella

pathogenicity islands (SPIs) 1 and 2 are present across all

Salmonella

, including this subspecies, although effectors sipA, sptP and arvA in SPI-1 and sseG and ssaI in SPI-2 appear to be lost in this lineage. SPI-20, encoding a type VI secretion system, is exclusive to this subspecies and is well maintained in all genomes sampled. A number of fimbral operons were identified, including the sas operon that appears to be a synapomorphy for this subspecies, while others exhibited more clade-specific patterns. This work reveals evolutionary patterns in

S. enterica

subsp.

arizonae

that make this subspecies a unique lineage within this very diverse species.
The Oregon Child Absenteeism Due to Respiratory Disease Study (ORCHARDS): Rationale, Objectives, and Design
Authors: Temte Jonathan L. , Barlow Shari , Goss Maureen , Temte Emily , Schemmel Amber , Maerz Bradley , Bell Cristalyne , Comp Lily , Arnold Mitchell , Breunig Kimberly , Clifford Sarah , Reisdorf Erik , Shult Peter , Wedig Mary , Haupt Thomas , Conway James , Gangnon Ronald , Fowlkes Ashley , Uzicanin Amra 
Published: February 2021

AbstractBackgroundInfluenza viruses pose significant disease burdens through annual seasonal outbreaks and unpredictable pandemics. Existing influenza surveillance programs have relied heavily on reporting of medically attended influenza (MAI). Continuously monitoring cause-specific school absenteeism may identify local acceleration of seasonal influenza activity. The Oregon Child Absenteeism Due to Respiratory Disease Study (ORCHARDS; Oregon, WI) implements daily school-based monitoring of influenza-like illness–specific student absenteeism (a-ILI) in pre-kindergarten through grade 12 schools and assesses this approach for early detection of accelerated influenza and other respiratory pathogen transmission in schools and surrounding communities.MethodsStarting in September 2014, ORCHARDS combined automated reporting of daily absenteeism within 6 schools and home visits to school children with acute respiratory infections (ARI). Demographic, epidemiological, and symptom data are collected along with respiratory specimens. Specimens are tested for influenza and other respiratory viruses. Household members can opt into a supplementary household transmission study. Community comparisons are possible using a pre-existing, long-standing, and highly effective influenza surveillance program, based on MAI at 5 primary care clinics in the same geographical area.ResultsOver the first 5 years, a-ILI occurred on 6,634 (0.20%) of 3,260,461 student school days. Viral pathogens were detected in 64.5% of the 1,728 children with ARI who received a home visit. Influenza was the most commonly detected virus, noted in 23.3% of ill students. Influenza (p<0.001) and adenovirus (P=0.004) were significantly associated with a-ILI.ConclusionORCHARDS uses a community-based design to detect influenza trends over multiple seasons and to evaluate the utility of absenteeism for early detection of accelerated influenza and other respiratory pathogen transmission in schools and surrounding communities. Initial findings suggest the study design is succeeding in collecting appropriate data to achieve study objectives.