bluuebunny/crossref_metadata_2025_split huggingface dataset:
Code-switching in parents’ everyday speech to bilingual infants
Authors: KREMIN Lena V. , ALVES Julia , ORENA Adriel John , POLKA Linda , BYERS-HEINLEIN Krista 
Published: May 2021

AbstractCode-switching is a common phenomenon in bilingual communities, but little is known about bilingual parents’ code-switching when speaking to their infants. In a pre-registered study, we identified instances of code-switching in day-long at-home audio recordings of 21 French–English bilingual families in Montreal, Canada, who provided recordings when their infant was 10 and 18 months old. Overall, rates of infant-directed code-switching were low, averaging 7 times per hour (6 times per 1,000 words) at 10 months and increasing to 28 times per hour (18 times per 1,000 words) at 18 months. Parents code-switched more between sentences than within a sentence; this pattern was even more pronounced when infants were 18 months than when they were 10 months. The most common apparent reasons for code-switching were to bolster their infant's understanding and to teach vocabulary words. Combined, these results suggest that bilingual parents code-switch in ways that support successful bilingual language acquisition.
Finnish inserted vowels: a case of phonologized excrescence
Authors: Karlin Robin 
Published: May 2021

AbstractIn this paper, I examine a case of vowel insertion found in Savo and Pohjanmaa dialects of Finnish that is typically called “epenthesis”, but which demonstrates characteristics of both phonetic excrescence and phonological epenthesis. Based on a phonological analysis paired with an acoustic corpus study, I argue that Finnish vowel insertion is the mixed result of phonetic excrescence and the phonologization of these vowels, and is related to second-mora lengthening, another dialectal phenomenon. I propose a gestural model of second-mora lengthening that would generate vowel insertion in its original phonetic state. The link to second-mora lengthening provides a unified account that addresses both the dialectal and phonological distribution of the phenomenon, which have not been linked in previous literature.
Evaluation of the Causes of Wet‐Season Dry Biases Over Amazonia in CAM5
Authors: Ma Hsi‐Yen , Zhang Kai , Tang Shuaiqi , Xie Shaocheng , Fu Rong 
Published: May 2021

AbstractThis study investigates the causes of pronounced low precipitation bias over Amazonia in the Community Atmosphere Model version 5 (CAM5), a common feature in many global climate models. Our analysis is based on a suite of 3‐day long hindcasts starting every day at 00Z from 1997 to 2012 and an AMIP simulation for the same period. The Amazonia dry bias appears by the second day in the hindcasts and is very robust for all the seasons with the largest bias magnitude during the wet season (December–February). The bias pattern and magnitude do not change much during different dynamical wind regimes on sub‐seasonal time scales. We further classify the diurnal cycle of precipitation near the LBA sites from observations and hindcasts into three convective regimes: no precipitation, late afternoon deep convection, and nighttime deep convection. CAM5 can only simulate the late afternoon convective regime and completely fails to simulate the nighttime convection, which is mostly from propagating convective systems originating from remote locations. CAM5 mainly underestimates precipitation in the late afternoon and nighttime convective regimes, which occur during ∼67% of wet season days and account for ∼75% of accumulated precipitation amount in observations. The persistent warm temperature bias and slightly higher moisture below 850 mb likely trigger deep convection too frequently, resulting in an earlier but weaker rainfall peak in the diurnal cycle. Furthermore, shallow convection may not effectively transport moisture from boundary layer to the free atmosphere, which also leads to weaker deep convection events.
Growth outcomes of small for gestational age preterm infants before and after implementation of an exclusive human milk-based diet
Authors: Fleig Lindsay , Hagan Joseph , Lee Martin L. , Abrams Steven A. , Hawthorne Keli M. , Hair Amy B. 
Published: May 2021

Abstract
Objective
Small for gestational age (SGA) preterm infants (PT) are at greatest risk for growth failure. Our objective was to assess the impact of an exclusive human milk diet (HUM) on growth velocities and neonatal morbidities from birth to discharge in a SGA population.

Study design
Multicenter, retrospective cohort study, subgroup analysis of SGA PT comparing a cow’s milk diet (CMD) with HUM diet.

Results
At birth 420 PT were classified as SGA (197 CMD group, 223 HUM group). Demographics and anthropometric measurements were similar. HUM group PT showed improvement in length Z score at discharge (p = 0.024) and reduction in necrotizing enterocolitis (NEC) (p = 0.004).

Conclusion
SGA PT fed a HUM diet had significantly decreased incidence of NEC, surgical NEC, and late-onset sepsis. Due to concerns about growth in a HUM diet, it is reassuring SGA infants fed the HUM diet had similar growth to CMD diet with trends toward improvement.
Dynamics of neutralizing antibody responses to SARS-CoV-2 in patients with COVID-19: an observational study
Authors: Xu Xin , Nie Sheng , Wang Yanqun , Long Quanxin , Zhu Hong , Zhang Xiaoyong , Sun Jian , Zeng Qinglang , Zhao Jincun , Liu Li , Li Ling , Huang Ailong , Hou Jinlin , Hou Fan Fan 
Published: May 2021

AbstractOur understanding of the protective immunity, particularly the long-term dynamics of neutralizing antibody (NAbs) response to SARS-CoV-2, is currently limited. We enrolled a cohort of 545 COVID-19 patients from Hubei, China, who were followed up up to 7 months, and determined the dynamics of NAbs to SARS-CoV-2 by using a surrogate virus neutralization test (sVNT). In our validation study, sVNT IC50 titers and the neutralization rate measured at a single dilution (1:20) were well correlated with FRNT titers (r = 0.85 and 0.84, respectively). The median time to seroconversion of NAbs was 5.5 days post onset of symptoms. The rate of positive sVNT was 52% in the first week, reached 100% in the third week, and remained above 97% till 6 months post onset. Quantitatively, NAbs peaked in the fourth week and only a quarter of patients had an estimated peak titer of >1000. NAbs declined with a half-time of 61 days (95% CI: 49–80 days) within the first two months, and the decay deaccelerated to a half-time of 104 days (95% CI: 86–130 days) afterward. The peak levels of NAbs were positively associated with severity of COVID-19 and age, while negatively associated with serum albumin levels. The observation that the low-moderate peak neutralizing activity and fast decay of NAbs in most naturally infected individuals called for caution in evaluating the feasibility of antibody-based therapy and vaccine durability. NAbs response positively correlated with disease severity, warning for the possibility of repeat infection in patients with mild COVID-19.
Extinction of anciently associated gut bacterial symbionts in a clade of stingless bees
Authors: Cerqueira Alan Emanuel Silva , Hammer Tobin J , Moran Nancy A , Santana Weyder Cristiano , Kasuya Maria Catarina Megumi , da Silva Cynthia Canêdo 
Published: May 2021

Abstract
Animal-microbe symbioses are often stable for millions of years. An example is the clade consisting of social corbiculate bees—honeybees, bumblebees, and stingless bees—in which a shared ancestor acquired specialized gut bacteria that subsequently diversified with hosts. This model may be incomplete, however, as few microbiomes have been characterized for stingless bees, which are diverse and ecologically dominant pollinators in the tropics. We surveyed gut microbiomes of Brazilian stingless bees, focusing on the genus Melipona, for which we sampled multiple species and biomes. Strikingly, Melipona lacks Snodgrassella and Gilliamella, bacterial symbionts ubiquitous in other social corbiculate bees. Instead, Melipona species harbor more environmental bacteria and bee-specific Starmerella yeasts. Loss of Snodgrassella and Gilliamella may stem from ecological shifts in Melipona or the acquisition of new symbionts as functional replacements. Our findings demonstrate the value of broadly sampling microbiome biodiversity and show that even ancient symbioses can be lost.
Pharmacogenetic genotype and phenotype frequencies in a large Danish population-based case-cohort sample
Authors: Lunenburg Carin A. T. C. , Thirstrup Janne P. , Bybjerg-Grauholm Jonas , Bækvad-Hansen Marie , Hougaard David M. , Nordentoft Merete , Werge Thomas , Børglum Anders D. , Mors Ole , Mortensen Preben B. , Gasse Christiane 
Published: May 2021

AbstractPharmacogenetics aims to improve clinical care by studying the relationship between genetic variation and variable drug response. Large population-based datasets could improve our current understanding of pharmacogenetics from selected study populations. We provide real-world pharmacogenetic frequencies of genotypes and (combined) phenotypes of a large Danish population-based case-cohort sample (iPSYCH2012; data of the Integrative Psychiatric Research consortium). The genotyped sample consists of 77,684 individuals, of which 51,464 individuals had diagnoses of severe mental disorders (SMD case-cohort) and 26,220 were individuals randomly selected from the Danish population (population cohort). Array-based genotype data imputed to 8.4 million genetic variants was searched for a selected pharmacogenetic panel of 42 clinically relevant variants and a CYP2D6 gene deletion and duplication. We identified 19 of 42 variants. Minor allele frequencies (MAFs) were consistent with previously reported MAFs, and did not differ between SMD cases and population cohorts. Almost all individuals carried at least one genetic variant (> 99.9%) and 87% carried three or more genetic variants. When genotypes were translated into phenotypes, also > 99.9% of individuals had at least one divergent phenotype (i.e. divergent from the common phenotypes considered normal, e.g. extensive metabolizer). The high number of identified individuals with at least one pharmacogenetic variant or divergent phenotype indicates the importance of pharmacogenetic panel-based genotyping. Combined CYP2C19-CYP2D6 phenotypes revealed that 72.7% of individuals had divergent phenotypes for one or both enzymes. As CYP2D6 and CYP2C19 have an important role in the metabolism of psychotropic drugs, this indicates the relevance of pharmacogenetic testing specifically in individuals using psychotropic drugs.
The protective role of MC1R in chromosome stability and centromeric integrity in melanocytes
Authors: Li Xin , Mao Weiwei , Chen Jie , Goding Colin R. , Cui Rutao , Xu Zhi-Xiang , Miao Xiao 
Published: May 2021

AbstractVariants in the melanocortin-1 receptor (MC1R) gene, encoding a trimeric G-protein-coupled receptor and activated by α-melanocyte-stimulating hormone (α-MSH), are frequently associated with red or blonde hair, fair skin, freckling, and skin sensitivity to ultraviolet (UV) light. Several red hair color variants ofMC1Rare also associated with increased melanoma risk.MC1Rvariants affect melanoma risk independent of phenotype. Here, we demonstrated that MC1R is a critical factor in chromosome stability and centromere integrity in melanocytes. α-MSH/MC1R stimulation prevents melanocytes from UV radiation-induced damage of chromosome stability and centromere integrity. Mechanistic studies indicated that α-MSH/MC1R-controlled chromosome stability and centromeric integrity are mediated by microphthalmia-associated transcription factor (Mitf), a transcript factor needed for the α-MSH/MC1R signaling and a regulator in melanocyte development, viability, and pigment production. Mitf directly interacts with centromere proteins A in melanocytes. Given the connection amongMC1Rvariants, red hair/fair skin phenotype, and melanoma development, these studies will help answer a question with clinical relevance “why red-haired individuals are so prone to developing melanoma”, and will lead to the identification of novel preventive and therapeutic strategies for melanomas, especially those with redheads.
Smoothened inhibition leads to decreased cell proliferation and suppressed tissue fibrosis in the development of benign prostatic hyperplasia
Authors: Liu Jianmin , Yin Jing , Chen Ping , Liu Daoquan , He Weixiang , Li Yan , Li Mingzhou , Fu Xun , Zeng Guang , Guo Yuming , Wang Xinghuan , DiSanto Michael E. , Zhang Xinhua 
Published: May 2021

AbstractBenign prostatic hyperplasia (BPH) is a common disease in aging males. It has been proven that the Hedgehog (HH) is implied as an effective and fundamental regulatory growth factor signal for organogenesis, homeostasis, and regeneration. Smoothened (SMO), as the major control point of HH signals, activates aberrantly in most human solid tumors. However, the specific function of SMO and its downstream glioma-associated oncogene (GLI) family in BPH has not been well understood. Here, we first revealed that the SMO cascade was upregulated in BPH tissues and was localized in both the stromal and the epithelium compartments of human prostate tissues. Cyclopamine, as a specific SMO inhibitor, was incubated with BPH-1 and WPMY-1, and intraperitoneally injected into a BPH rat model established by castration with testosterone supplementation. SMO inhibition could induce cell apoptosis, cell cycle arrest at the G0/G1 phase, and a reduction of tissue fibrosis markers, both in vitro and in vivo. Finally, a tissue microarray, containing 104 BPH specimens, was constructed to analyze the correlations between the expression of SMO cascade and clinical parameters. The GLI2 was correlated positively with nocturia and negatively with fPSA. The GLI3 was in a positive relationship with International Prostate Symptom Score and nocturia. In conclusion, our study suggested that SMO cascade could play important roles in the development of BPH and it might be rediscovered as a promising therapeutic target for BPH.
Economic damages from Hurricane Sandy attributable to sea level rise caused by anthropogenic climate change
Authors: Strauss Benjamin H. , Orton Philip M. , Bittermann Klaus , Buchanan Maya K. , Gilford Daniel M. , Kopp Robert E. , Kulp Scott , Massey Chris , Moel Hans de , Vinogradov Sergey 
Published: May 2021

AbstractIn 2012, Hurricane Sandy hit the East Coast of the United States, creating widespread coastal flooding and over $60 billion in reported economic damage. The potential influence of climate change on the storm itself has been debated, but sea level rise driven by anthropogenic climate change more clearly contributed to damages. To quantify this effect, here we simulate water levels and damage both as they occurred and as they would have occurred across a range of lower sea levels corresponding to different estimates of attributable sea level rise. We find that approximately $8.1B ($4.7B–$14.0B, 5th–95th percentiles) of Sandy’s damages are attributable to climate-mediated anthropogenic sea level rise, as is extension of the flood area to affect 71 (40–131) thousand additional people. The same general approach demonstrated here may be applied to impact assessments for other past and future coastal storms.
Nuclear organisation and replication timing are coupled through RIF1–PP1 interaction
Authors: Gnan Stefano , Flyamer Ilya M. , Klein Kyle N. , Castelli Eleonora , Rapp Alexander , Maiser Andreas , Chen Naiming , Weber Patrick , Enervald Elin , Cardoso M. Cristina , Bickmore Wendy A. , Gilbert David M. , Buonomo Sara C. B. 
Published: May 2021

AbstractThree-dimensional genome organisation and replication timing are known to be correlated, however, it remains unknown whether nuclear architecture overall plays an instructive role in the replication-timing programme and, if so, how. Here we demonstrate that RIF1 is a molecular hub that co-regulates both processes. Both nuclear organisation and replication timing depend upon the interaction between RIF1 and PP1. However, whereas nuclear architecture requires the full complement of RIF1 and its interaction with PP1, replication timing is not sensitive to RIF1 dosage. The role of RIF1 in replication timing also extends beyond its interaction with PP1. Availing of this separation-of-function approach, we have therefore identified in RIF1 dual function the molecular bases of the co-dependency of the replication-timing programme and nuclear architecture.
A luminescent view of the clickable assembly of LnF3 nanoclusters
Authors: Zhou Jie , Wei Yang , Pan Yue , Wang Yue , Yuan Ze , Zhang Fan , Song Hao , Yue Jingyi , Su Haiquan , Xie Xiaoji , Huang Ling 
Published: May 2021

AbstractNanoclusters (NCs) bridge the gap between atoms and nanomaterials in not only dimension but also physicochemical properties. Precise chemical and structural control, as well as clear understanding of formation mechanisms, have been important to fabricate NCs with high performance in optoelectronics, catalysis, nanoalloys, and energy conversion and harvesting. Herein, taking advantage of the close chemical properties of Ln3+ (Ln = Eu, Nd, Sm, Gd, etc.) and Gd3+–Eu3+ energy transfer ion-pair, we report a clickable LnF3 nanoparticle assembly strategy allowing reliable fabrication of diversely structured NCs, including single-component, dimeric, core-shelled/core-shell-shelled, and reversely core-shelled/core-shell-shelled, particularly with synergized optical functionalities. Moreover, the purposely-embedded dual luminescent probes offer great superiority for in situ and precise tracking of tiny structural variations and energy transfer pathways within complex nanoarchitectures.
Electrically connected spin-torque oscillators array for 2.4 GHz WiFi band transmission and energy harvesting
Authors: Sharma Raghav , Mishra Rahul , Ngo Tung , Guo Yong-Xin , Fukami Shunsuke , Sato Hideo , Ohno Hideo , Yang Hyunsoo 
Published: May 2021

AbstractThe mutual synchronization of spin-torque oscillators (STOs) is critical for communication, energy harvesting and neuromorphic applications. Short range magnetic coupling-based synchronization has spatial restrictions (few µm), whereas the long-range electrical synchronization using vortex STOs has limited frequency responses in hundreds MHz (<500 MHz), restricting them for on-chip GHz-range applications. Here, we demonstrate electrical synchronization of four non-vortex uniformly-magnetized STOs using a single common current source in both parallel and series configurations at 2.4 GHz band, resolving the frequency-area quandary for designing STO based on-chip communication systems. Under injection locking, synchronized STOs demonstrate an excellent time-domain stability and substantially improved phase noise performance. By integrating the electrically connected eight STOs, we demonstrate the battery-free energy-harvesting system by utilizing the wireless radio-frequency energy to power electronic devices such as LEDs. Our results highlight the significance of electrical topology (series vs. parallel) while designing an on-chip STOs system.
A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
Authors: Xun Guanhua , Lane Stephan Thomas , Petrov Vassily Andrew , Pepa Brandon Elliott , Zhao Huimin 
Published: May 2021

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.
RAS mutations drive proliferative chronic myelomonocytic leukemia via a KMT2A-PLK1 axis
Authors: Carr Ryan M. , Vorobyev Denis , Lasho Terra , Marks David L. , Tolosa Ezequiel J. , Vedder Alexis , Almada Luciana L. , Yurcheko Andrey , Padioleau Ismael , Alver Bonnie , Coltro Giacomo , Binder Moritz , Safgren Stephanie L. , Horn Isaac , You Xiaona , Solary Eric , Balasis Maria E. , Berger Kurt , Hiebert James , Witzig Thomas , Buradkar Ajinkya , Graf Temeida , Valent Peter , Mangaonkar Abhishek A. , Robertson Keith D. , Howard Matthew T. , Kaufmann Scott H. , Pin Christopher , Fernandez-Zapico Martin E. , Geissler Klaus , Droin Nathalie , Padron Eric , Zhang Jing , Nikolaev Sergey , Patnaik Mrinal M. 
Published: May 2021

AbstractProliferative chronic myelomonocytic leukemia (pCMML), an aggressive CMML subtype, is associated with dismal outcomes. RAS pathway mutations, mainly NRASG12D, define the pCMML phenotype as demonstrated by our exome sequencing, progenitor colony assays and a Vav-Cre-NrasG12D mouse model. Further, these mutations promote CMML transformation to acute myeloid leukemia. Using a multiomics platform and biochemical and molecular studies we show that in pCMML RAS pathway mutations are associated with a unique gene expression profile enriched in mitotic kinases such as polo-like kinase 1 (PLK1). PLK1 transcript levels are shown to be regulated by an unmutated lysine methyl-transferase (KMT2A) resulting in increased promoter monomethylation of lysine 4 of histone 3. Pharmacologic inhibition of PLK1 in RAS mutant patient-derived xenografts, demonstrates the utility of personalized biomarker-driven therapeutics in pCMML.
Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections
Authors: Zhang Chao , Xu Cong , Dai Wenlong , Wang Yifan , Liu Zhi , Zhang Xueyang , Wang Xuesong , Wang Haikun , Gong Sitang , Cong Yao , Huang Zhong 
Published: May 2021

AbstractEnterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP and virion and in neutralizing different EV-D68 strains. A combination of 2H12 and 8F12 exhibits balanced and potent neutralization effects and confers broader protection in mice than single MAbs when given at onset of symptoms. Cryo-EM structures of EV-D68 virion complexed with 2H12 or 8F12 show that both antibodies bind to the canyon region of the virion, creating steric hindrance for sialic acid receptor binding. Additionally, 2H12 binding can impair virion integrity and trigger premature viral uncoating. We also capture an uncoating intermediate induced by 2H12 binding, not previously described for picornaviruses. Our study elucidates the structural basis and neutralizing mechanisms of the 2H12 and 8F12 MAbs and supports further development of the 2H12/8F12 cocktail as a broad-spectrum therapeutic agent against EV-D68 infections in humans.