bluuebunny/crossref_metadata_2025_split huggingface dataset:
Actin nucleation safeguards single-stranded DNA and promotes replication fork protection
Authors: Nieminuszczy Jadwiga , Martin Peter , Broderick Ronan , Krwawicz Joanna , Kanellou Alexandra , Bousgouni Vicky , Bakal Chris , Woodward Beth , Shackley Fiona , Aguilera Andrés , Stewart Grant , Vyas Yatin , Niedzwiedz Wojciech 
Published: September 2021

Abstract
Accurate genome replication is essential for all life and a key mechanism of disease prevention, underpinned by the ability of cells to respond to replicative stress and protect stalled replication forks. All such responses rely on the formation of Replication Protein A (RPA)-ssDNA complexes, yet supra-physiological binding of RPA to ssDNA is toxic. How cells regulate RPA availability to promote fork protection and genome stability is largely unknown. Here we establish that during replication excess RPA is sequestered by monomeric actin and released upon replicative stress through transition to polymeric actin state. Impairment in actin nucleation leads to RPA sequestration, deprotection of ssDNA generated at the stressed forks and consequently, catastrophic fork collapse and hypersensitivity to replication inhibitors. In line with this, we show that increasing RPA load is sufficient to restore efficient fork protection in actin polymerization mutants. Collectively, this work identifies a simple yet robust RPA-buffering mechanism regulating its availability to bind ssDNA and protect replication forks against nucleolytic degradation. Inhibition of this pathway could be of therapeutic interest in treatment of cancers.
Phthalate Exposure Enhances Incidence of Urinary Incontinence: US NHANES, 2003-2004 and 2005-2006
Authors: Yi Xianyanling , Jin Kun , Qiu Shi , Xiong Xingyu , Zhang Tianyi , Peng Ge , Liao Dazhou , Zheng Xiaonan , Xu Hang , Li Hong , Yang Lu , Wei Qiang , Ai Jianzhong 
Published: September 2021

Abstract
Objective: The aim of this study is to investigate the associations between phthalate exposure and UI in a nationally representative sample of US adults.Methods: Cross-sectional data from the National Health and Nutrition Examination Survey (NHANES) database was used for analysis. In total, 2,818 participants with measurements for phthalate metabolites and complete UI questionnaire data were enrolled in our study. Further, seven phthalate metabolites were measured, which were obtained from urine samples and creatinine-standardized in the subsequent analyses. After divided these phthalate metabolites into three groups, multivariable regression models were performed to evaluate the association between phthalate metabolites and UI rates. Moreover, interaction analyses and subgroup analyses stratified by gender were performed.Results: In these seven phthalate metabolites, high level of mono-carboxynonyl phthalate (MCNP), mono-carboxyoctyl phthalate (MCOP), mono-isobutyl phthalate (MiBP), mono-n-butyl phthalate (MBP) and mono-3-carboxypropyl phthalate (MCPP) showed increased risk of UI [odds ratio (OR) = 1.52, 1.42, 1.43, 1.50, 1.51, respectively, all p value < 0.05]. Trend test showed that incidence of UI increased significantly with concentration. A higher incidence of UI among participants was observed in the maximal tertile of phthalate when comparing with the lowest tertile. Subgroup analysis found that different phthalates have varying influence for different types of UI. Moreover, the analyses stratified for sex indicated that the high concentrations of MCNP and median concentrations of MCCP were associated with increase of the odds of UI in women and in men, respectively.Conclusion: Overall, the exposure to phthalates was positively associated with UI among US adults. Notably, different phthalates have varying influence for different types of UI, and male and female exposure to phthalate could result in the different prevalence of UI.
Feasibility and Safety of Drug-Coated Balloon-Only Angioplasty for De Novo Ostial Lesions of Left Anterior Descending Artery: Two Center’s Retrospective Study
Authors: Li Chuang , Ding Xuebo , Wang Lefeng , Li Kuibao , Yang Xinchun , Liu Liping , Xu Li 
Published: September 2021

Abstract
Background:There is limited evidence of drug-coated balloon (DCB) only angioplasty in percutaneous treatment of complex de novo ostial coronary lesions. The major objective of our study is to explore the feasibility and test safety of this innovative approach in ostial lesions of left anterior descending artery (LAD). Methods:Patients treated with paclitaxel DCB or second-generation drug-eluting stent (DES) were retrospectively enrolled from two different large centers. The primary endpoints were defined as major adverse cardiovascular events (MACE) composed of cardiovascular death, target lesion revascularization (TLR), target vessel revascularization (TVR), and recurrent myocardial infarction related to target artery occlusion. Cox regression analysis were used to identify risk factors for MACE and propensity score matching is performed to minimize the selection bias.Results:A total of 53 patients were treated with paclitaxel DCB and 336 patients with DES in ostial lesions of LAD were recruited. In accordance with propensity score matching, 49 patients treated with DCB-only coordinated with 49 ones with the strategy of DES. After average follow-up time of 10 months, the rate of MACE trended to lower in DCB-only angioplasty treatment arm and triggered by post-procedure TLR (MACE: 6% vs. 4%, p=0.65; TLR: 2% vs. 4%, p=0.56). Cox regression analysis indicated that not DCB-only angioplasty was considered as an independent risk factor for adverse events after adjustment for cofound risk factors (HR: 1.748, p=0.48).Conclusions:Use of DCB-only approach in treatment of isolated ostial LAD disease could be an innovative and safe strategy without additional risk of aggressive progression of left circumflex artery.
XLOC-040580, a Novel lncRNA, Modulates Porcine Zygote Activation Progress
Authors: Liu Mengxin , Li Enhong , Mu Haiyuan , Zhao Zimo , Gao Jie , Gao Dengfeng , Liu Zhiyu , Han Jianyong , Zhong Liang , Cao Suying 
Published: September 2021

Abstract
Background: Long noncoding RNAs (lncRNAs) play an important role in porcine preimplantation embryonic development, but the regulatory mechanism of lncRNAs during zygote activation (ZGA) remains unclear.Methods: We analyzed the transcriptome data of porcine fetal fibroblasts (PEF), porcine induced pluripotent stem cells (piPSCs) and embryos at different developmental stages before implantation, and found that lncRNAs specifically expressed at ZGA stage including XLOC-040580, and further predicted its target genes TPRA1 and BCL2L1. XLOC-040580 was knocked down by microinjection and the expression of ZGA related genes was detected by qRT-PCR. Microinjection of siRNA corresponding to target genes at the 1-cells, the blastocyst development rate was counted, and single cell sequencing was performed to analyze the regulatory network of target genes and its related mechanisms.Results: By analyzing the transcriptome data of porcine preimplantation embryos, we found that XLOC-040580 was specifically expressed during zygote activation. Knockdown of XLOC-040580 resulted in decreased blastocyst development rate, total blastocyst cell number and TE cell number. XLOC-040580 target genes TPRA1 and BCL2L1 are also specifically expressed in ZGA stage. The knockdown of XLOC-040580 target genes also blocked porcine embryonic development and affected the gene expression of ZGA-related pathways such as cell cycle, histone modification and trophoblast formation during porcine embryonic development.Conclusion: We found and confirmed that lncRNA XLOC-040580 played a key role in the ZGA process, and its target genes (TPRA1, BCL2L1) also coordinated early embryonic development.
A Comparison of Blood Plasma Exosome Enrichment Strategies for Proteomic Analysis
Authors: Turner Natalie P. , Abeysinghe Pevindu , Cheung Keith A. Kwan , Vaswani Kanchan , Logan Jayden , Sadowski Pawel , Mitchell Murray D. 
Published: September 2021

Abstract
Proteomic analysis of exosomes (EX) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Our group has performed a comprehensive study of multi-dimensional enrichment methods to determine their efficiency for protein isolation. Methods were evaluated for their capacity to a) successfully isolate and enrich EX from blood plasma, b) minimise the presence of highly abundant plasma proteins, and c) result in the optimum representation of EX proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS). Blood plasma from four animals (Bos taurus) of similar physical attributes and genetics were used. Three methods of EX enrichment were utilised: ultracentrifugation (UC), size-exclusion chromatography (SEC), and ultrafiltration (UF). These enrichment methods were combined to create four groups for methodological evaluation: UC+SEC, UC+SEC+UF, SEC+UC and SEC+UF. UC+SEC yielded the highest number of protein IDs. Plasma protein identification was the least in SEC+UC, but this method yielded the lowest number of protein IDs overall. UC+SEC+UF decreased EX protein ID and did not improve purity compared to UC+SEC. Our data suggest that the method and sequence of EX enrichment strategy impacts protein ID, which may influence the outcome of biomarker discovery studies.
The perioperative outcomes of robot-assisted radical cystectomy with intracorporeal urinary diversion compared with laparoscopic radical cystectomy with extracorporeal urinary diversion at a single institution.
Authors: Suzuki Atsuto , Muraoka Kentaro , Tatenuma Tomoyuki , Osaka Kimito , Yokomizo Yumiko , Hayashi Narihiko , Hasumi Hisashi , Ito Yusuke , Kondo Keichi , Nakaigawa Noboru , Makiyama Kazuhide 
Published: September 2021

Abstract
Background
The number of facilities adapting intracorporeal urinary diversion (ICUD) using robots instead of extracorporeal urinary diversion (ECUD) is increasing, but the clinical evidence is limited.
Methods
We retrospectively analyzed 26 consecutive patients who underwent ICUD with an ileal conduit following robot-assisted radical cystectomy (RARC) between 2018 and 2020 (RARC + ICUD group), and compared them with 26 consecutive patients who underwent ECUD with an ileal conduit following laparoscopic radical cystectomy (LRC) between 2012 and 2019 (LRC + ECUD group) at Yokohama City University.
Results
In both groups, the patient background was similar except for age and neoadjuvant chemotherapy. In the RARC + ICUD group vs. the LRC + ECUD group, the median total operation time was 516 min vs. 532.5 min (p = 0.217), the time to cystectomy was 163 min vs. 194.5 min (p = 0.007), and the time of urinary diversion with an ileal conduit was 161 min vs. 201.5 min (p < 0.001). The postoperative maximum value of C-reactive protein was 6.98 mg/L vs. 12.46 mg/L (p = 0.001). The median number of days to oral intake was 3 days vs. 5 days (p = 0.003). The median length of hospital stay was 17 days vs. 32 days (p < 0.001). The postoperative complication rate (within 90 days) was 23.1% vs. 42.3% (p = 0.237). Clavien-Dindo classification ≥ 3 was noted in 1 vs. 4 patients (p = 0.350). The median number of dissected lymph nodes was 20 vs. 15 (p = 0.008).
Conclusions
RARC + ICUD was superior to LRC + ECUD in terms of time to cystectomy and ileal conduit urinary diversion, invasiveness, and lymph node dissection. We consider ICUD with an ileal conduit following RARC to be the more advantageous procedure.
Spine MRI Patterns And Predictors of Disability In Patients With Lower Back Pain. A Prospective Cross-Sectional Study at University of Gondar Comprehensive Specialized Hospital, North West Ethiopia. 2020.
Authors: Gebrewold Yonathan , Tesfaye Bati 
Published: September 2021

Abstract
Background: Low back pain (LBP) has become one of the most serious public health problems with substantial socioeconomic implication. Degenerative disc disease one of the commonest cause of LBP. Magnetic resonance imaging (MRI) is routinely utilized in evaluation patients with degenerative changes of the lumbar spine. However there are contradictors reports with regards to association of MRI findings of lumbar spine and patients’ symptoms.Objective: This study is aimed determine correlation of degree of disability with lumbar spine MRI findings in patients with LBP at University of Gondar comprehensive Specialized Hospital (UoGCSH), North West Ethiopia, 2020.Methods and Materials. A prospective cross-sectional study was conducted on 72 consecutively enrolled patients with lower back pain who underwent lumbar MRI scan. Degree of disability was measured using Oswestry disability Index (ODI) questionnaire translated to local language. Association between lumbar spine MRI parameters and ODI score and category was tested using Spearman’s rank correlation coefficient and Chi square tests. Results The mean age the study subjects was 43.81±1.88 years (range 22-83 years). 59.7% of the study population were Female. In terms of ODI category, most fell under minimal and moderate disability 33 (45.8%) and 25(34.7%) respectively. Disc bulge (81.9%) and foraminal stenosis were the most frequent MRI abnormalities detected. ODI score showed weak correlation with grade of spinal canal stenosis. Foraminal stenosis grade was not correlated ODI.Conclusion: The clinical relevance of MRI findings in patients with degenerative disc disease is limited and MRI should be sparingly ordered in evaluation of these patients particularly in resource constrained settings.
Applying Four-Steps Characteristic Ion Filtering with HPLC-QE Orbitrap MS Approach to Rapid Characterize Compound Structure s and Determine Major Representative Components in Modified Tabusen-2 Decoction
Authors: Zhao yu , Dong xin , Feng qianxi , Dong rui , Bu ren , Xue Peifeng , Qu bi 
Published: September 2021

Abstract
Background: Modified Tabusen-2 decoction (MTBD) is Traditional Chinese Mongolia Medicine, it is consist of “Lancitou” “Duzhong” “Sanqi” and “Honghua”. MTBD is mainly used to treat osteoporosis. However, the precise material basis of this prescription is not yet fully elucidated, which affects its modernization developmentMethods: We establish a new HPLC-QE Orbitrap MS technology with four-steps characteristic ion filtering (FSCIF) strategy to quickly and effectively identify the structural features of MTBD, and determine the representative compounds content. Then the quantitative method of nineteen main components in MTBD were built, and eight batches MTBD were determined as well. Results: By using the FSCIF strategy, a total of 143 compounds were unambiguously or tentatively identified, including 5 compounds were first reported in MTBD. Nineteen representative components were simultaneously quantified by HPLC-QE Orbitrap MS, and it is suitable for eight batches of MTBD. Methodology analysis showed that the assay method of 19 compounds (including three pairs of isomers) with a good repeatability, accuracy, and stability. Conclusion: The method established above was successfully applied to assess the quality of MTBD extracts, which prove that the concentrations of 19 compounds varied significantly in different batches of MTBD extracts. Collectively, our findings enhance our molecular understanding of the MTBD formulation and will allow us to control its quality in a better way. At the same time, this study can promote the development and utilization of ethnic medicine.
Up-Regulated Galectin-1 in Angiostrongylus Cantonensis L5 Reduces Body Fat and Increases Oxidative Stress Tolerance
Authors: Sun Weiwei , Yan Xiumei , Qiao Aijun , Zhang Yuanjiao , Yang Ling , Huang Huicong , Shi Hongfei , Yan Baolong 
Published: September 2021

Abstract
Background: Angiostrongylus cantonensis L5, parasitizing in human cerebrospinal fluid, leads to eosinophilic meningitis, which is attributed to tissue inflammatory responses caused primarily by high percentage of eosinophils. Eosinophils are also involved in helminthic killing, using the peroxidative oxidation and hydrogen peroxide (H2O2) generated by dismutation of superoxide produced during respiratory burst. In contrast, helminthic worms have evolved to attenuate eosinophil-mediated tissue inflammatory responses for their survival. In previous study, we have demonstrated the extracellular function of Acan-Gal-1 in inducing the apoptosis of macrophages. And here, the intracellular functions of Acan-Gal-1 were investigated with the aim to further reveal the mechanism of A. cantonensis L5 worms surviving in the central nervous system of human from inflammatory responses. Methods: Bioinformatics were used to analyse the structural characterisation of Acan-Gal-1; qRT-PCR and microinjection were performed to detect the expression patterns of Acan-gal-1; microinjection was performed to construct transgenic worms; oxidative stress assay and Oil Red O fat staining were used to determine the functions of Acan-Gal-1.Results: The results showed that Acan-Gal-1 was expressed ubiquitously and mainly localized in cuticle, and it was up-regulated in both L5 and adult worm. N2 worms expressing pCe-Acan-gal-1::Acan-gal-1::rfp, with lipid deposition reduced, were significantly resistant to oxidative stress. lec-1 mutant worms, with lipid deposition increased, showed susceptible to oxidative stress, and this phenotype could be rescued by expressing pCe-Acan-gal-1::Acan-gal-1::rfp. And fat-6;fat-7 double-mutant worms expressing pCe-Acan-gal-1::Acan-gal-1::rfp showed no significant changes in oxidative stress tolerance.Conclusion: In C. elegans worms, up-regulated Acan-Gal-1 plays a defensive role against damage due to oxidative stress for worm survival through reducing fat deposition. And this might indicate the mechanism of A. cantonensis L5 worms, with Acan-Gal-1 up-regulated, surviving in the central nervous system of human from immune attack of Eosinophil.
The Changes of Cancer Patients' Perception, Attitude and Clinical Practice in Complementary and Alternative Medicine during COVID-19: A Multicenter Before-After Cross-Sectional Study
Authors: Wang Yichao , Yang Geliang , Gu Zhan , Wang Chengyan , Zhang Huiqing , Xu Ling 
Published: September 2021

Abstract
Background: With the rapid spread of COVID-19 worldwide, the clinical practice in cancer treatment of complementary and alternative medicine (CAM) becomes significantly different. This study was designed to explore the changes of China’s cancer patients’ perception, attitude and clinical practice in CAM after the pandemic of COVID-19. Methods: A multicenter before-after cross-sectional study was conducted, including 448 cancer patients by a survey. Results: For perception, half of the cancer patients (51.2%) reported they had received adequate information in CAM cancer treatment and had a great expectation in medical appointment pattern (40.2%). For attitudes, most of the cancer patients (79.4%) had full of confidence in CAM cancer treatment and showed a strong desire to learn more about CAM (70.2%). Nearly half of the cancer patients (44.0%) presented their oncology clinics had been greatly affected by COVID-19, however, most (82.8%) refused to the online medical service pattern. For clinical practice, Chinese herbal medicine was widely used before (89.5%) / after (90.3%) the epidemic of COVID-19 and the main purpose of receiving CAM therapy was to improve immune system (71.8%). The frequency of patients’ visiting the clinic decreased significantly (P < 0.001), and most (90.0%) didn’t change their follow-up pattern. The majority of the cancer patients (83.3%) were identified as CAM users and the only predictive factor was national health insurance in the multivariable logistic regression model. Conclusion: China’s cancer patients show more interest and confidence in treating cancer by CAM, and the new medical pattern such as medical appointment, has been gradually accepted during the COVID-19 pandemic. However, the cancer patients’ perception in CAM and online medical service pattern still need to be improved.
Glycyrrhiza Glabra and Platycodon Grandiflorus are Alternative Treatment Options for Vocal Fold Nodules
Authors: Lee I-Yun , Chang Shen-Fu , Wu Chin-Yuan , Lin Yu-Shi , Tsai Yao-Te , Tsai Ming-Shao , Chang Geng-He , Yang Ming-Yu , Yang Yao-Hsu , Yang Pei-Rung , Hsu Cheng-Ming 
Published: September 2021

Abstract
Background: Vocal fold nodules (VFN) are one of the main causes of hoarseness. In this study, we try to figure out an alternative treatment from our clinical experience summary.Methods: We retrospectively reviewed VFN patients who received traditional Chinese medicine (TCM) treatments from July 2018 to August 2020 and traced their Chinese Voice Handicap Index-10 (VHI-C10) and multidimensional voice program (MDVP) analysis results. Also, we induce inflammatory response of porcine vocal fold epithelial (PVFE) cells with 50 ng/mL TNF-alpha. The inflamed PVFE cells were separately cultured in the aqueous extract of Glycyrrhiza glabra and Platycodon grandiflorus. Results: For these patients (n = 22), the average VHI-C10 score decreased from 17.6 to 6.6 (p < 0.001). MDVP analysis revealed improvements in jitter, shimmer, noise–harmonic ratio, and GRBAS scoring system. Analysis of prescription patterns revealed that the most commonly used herbs were Licorice and Platycodon grandiflorus. In the MTT assay of PVFE cells, no adverse effects of our extracts were observed at doses of 1­–200 ug/mL. Western blot analysis revealed downregulation of p65 and mitogen-activated protein kinase pathway proteins.Conclusions: The results from both the clinical and in vitro aspects of this study revealed that the herbs G. glabra and P. grandiflorus may offer beneficial outcomes as alternative treatments for VFNs.
Phytochemical Analysis, Computational Modeling and Experimental Evaluations of Avicennia Marina Anti-cancer Activity on Breast, Ovarian and Cervical Cancer Cell Lines Running Title: Anti-cancer Activity of Avicennia Marina
Authors: Afshar Alireza , Khoradmehr Arezoo , Zare Masoud , Baghban Neda , Mohebbi Gholamhossein , Barmak Alireza , Khatami Mohsen , Mahmudpour Mehdi , Daneshi Adel , Bargahi Afshar , Azari Hossein , Nabipour Iraj , Ullah Mujib , Anvari Morteza , Tamadon Amin 
Published: September 2021

Abstract
Background: Avicennia marina, the gray mangrove, is an herbal source of bioactive anti-cancer compounds.Purpose: The present study aimed to evaluate phytochemical compositions of ethanol and ethyl acetate extracts of A. marina leaves and experimental study and computational modeling of their anti-cancer activity on breast, ovarian and cervical cancer cell lines.Study design: Phytochemical analysis, computational modeling and experimental in vitro evaluationMethods: Phytochemical analysis and GC-MS analysis were used to detect phenolic and flavonoid contents in ethanol and ethyl acetate extracts of A. marina leaves. Cell proliferation, viability, cycle and western blot assays of the extracts were performed on MCF-7, OVCAR3, and HeLa. Computational modeling done to detect effective compounds.Results: The extracts of A. marina leaves had high phenolic and flavonoid contents. In GC-MS analysis of the extracts, anti-cancer and anti-proliferative compounds were detected. Moreover, after treatment of MCF-7, OVCAR3, and HeLa, separately, the MTT assay, cell proliferation assay, and cell viability assays showed anti-proliferative activity, decreasing of cell population and decreasing cell viability, respectively. In addition, the cell cycle analysis showed that the S phase of the cell cycle increased in MCF-7. Moreover, the western blot analysis showed that the pro-apoptotic cell effectors such as Bax and caspase-1, -3, and -7 increased. Computational results of affinity of ligands detected by GC-MS compounds and stimulated apoptosis effectors detected by western blot showed five molecules in A. marina leaves playing role in OVCAR3 and HeLa apoptosis.Conclusion: The extracts of A. marina leaves have anti-cancer effects on breast, ovarian and cervical cancer cells via cell cycle arrest or apoptotic mechanisms.