bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel non-invasive murine model for rapidly testing drug activity via inhalation administration against Mycobacterium tuberculosis
Authors: Tian Xirong , Gao Yamin , Li Chunyu , Ma Wanli , Zhang Jingran , Ju Yanan , Ding Jie , Zeng Sanshan , Hameed H. M. Adnan , Aung Htin Lin , Zhong Nanshan , Cook Gregory M. , Hu Jinxing , Zhang Tianyu 
Published: January 2025

The efficacy of many compounds against Mycobacterium tuberculosis is often limited when administered via conventional oral or injection routes due to suboptimal pharmacokinetic characteristics. Inhalation-based delivery methods have been investigated to achieve high local therapeutic doses in the lungs. However, previous models, typically employing wild-type M. tuberculosis strains, were intricate, time-consuming, labor-intensive, and with poor reproducibility. In this study, we developed an autoluminescence-based inhalation administration model to evaluate drug activity by quantifying relative light units (RLUs) emitted from live mice infected with autoluminescent M. tuberculosis. This novel approach offers several advantages: (1) it eliminates the need for anesthesia in mice during administration and simplifies the instrument manipulation; (2) it is cost-effective by utilizing mice instead of larger animals; (3) it shortens the time from several months to 16 or 17 days for obtaining result; (4) it is non-invasive by directly measuring the live RLUs of mice as a surrogate marker for colony-forming units for in vivo drug activity testing; (5) up to six mice can be administrated daily and simultaneously, even 2–3 times/day; (6) results are relatively objective and reproducible results minimizing human factors. Proof-of-concept experiments demonstrated that inhalable rifampicin, isoniazid, and ethambutol showed anti-M. tuberculosis activity at concentrations as low as 0.5, 0.5, and 0.625 mg/mL, respectively, as evidenced by comparing the live RLUs of mice. Furthermore, consistency between RLUs and colony-forming units of the autoluminescent M. tuberculosis in lungs reaffirms the reliability of RLUs as an indicator of drug efficacy, highlighting the potential of this approach for accurately assessing anti-M. tuberculosis activity in vivo. This autoluminescence-based, non-invasive inhalation model offers a substantial reduction in the time, effort, and cost required for evaluating the efficacy of screening new drugs and repurposing old drugs in vivo via inhalation administration.
Effect of bromelain on ischemia-reperfusion injury in the torsion model created in polycystic and normal ovarian tissues
Authors: Ulusoy Tangul Sevgi , Onat Taylan , Aydoğan Kirmizi Demet , Doganyigit Zuleyha , Kaymak Emin , Oflamaz Aslı , Şenayli Atilla , Somuncu Salih 
Published: January 2025

PurposeDue to its increased volume, polycystic ovarian tissue is more prone to torsion than normal ovarian tissue. In treating ovarian torsion, detorsion is applied to ensure oxygenation of hypoxic tissues. However, the resulting oxygen radicals cause tissue damage. Bromelain is a substance obtained from pineapple, and studies in the literature show it is used as an antioxidant. This study aimed to evaluate the damage caused by ischemia-reperfusion (I/R) in the torsion-detorsion model created in normal and polycystic ovarian tissue and investigate the role of bromelain in this damage.MethodsPolycystic ovarian tissue was created by applying dihydroepiandrosterone sulfate to rats. Afterward, a torsion-detorsion model was used for all rats. The rats were divided into six groups: the polycystic ovary sham-operated group (P-S), the normal ovary sham-operated group (N-S), the polycystic ovary ischemia/reperfusion group (P-IR), the normal ovary ischemia/reperfusion group (N-IR), the polycystic ovary ischemia/reperfusion group treated with bromelain (P-IRB), and the normal ovary ischemia/reperfusion group treated with bromelain (N-IRB). After the procedure, tissues were collected for histopathological examination, and MDA, TUNEL, and NF-κB levels were measured.ResultsThis study detected significant decreases in MDA and NF-κB levels and apoptotic cell numbers assessed by TUNEL staining in groups with IR damage and given bromelain compared to the control groups. The number of TUNEL-positive cells was found to be highest in the P-IR group (8.80 ± 2.98) and significantly lower in the bromelain-administered P-IRB (1.04 ± 1.09) and N-IRB (0.52 ± 0.58) groups (p&lt; 0.05). NF-κB expression was also high in P-IR and N-IR groups, while it was significantly decreased in bromelain-treated groups (P-IRB and N-IRB) (p&lt; 0.05). In addition, IR damage was more pronounced in polycystic ovary tissue than in normal ovary tissue.ConclusionIschemia perfusion damage may be more pronounced in polycystic ovarian tissue than in normal ovarian tissue. Bromelain may be preferred to prevent I/R injury caused by ovarian torsion. It is also thought that bromelain may function in treating polycystic ovaries, and further studies can be conducted on this subject.
mHealth-based exercise vs. traditional exercise on pain, functional disability, and quality of life in patients with knee osteoarthritis: a systematic review and meta-analysis of randomized controlled trials
Authors: Tang Liang , Wang Meng-Ming , Wang He-Xia , He Xiao-Ya , Jiang Yue-Shuai 
Published: January 2025

ObjectiveThis study aims to compare the efficacy of mHealth-based exercise interventions with traditional exercise in improving pain intensity, functional disability, and quality of life in patients suffering from knee osteoarthritis (OA).MethodRandomized controlled trials (RCTs) published from their inception to 23 August 2024 were searched in Cochrane, Embase, Medline, Web of Science. Reviewer pairs independently extracted data and evaluated bias using the Cochrane Risk of Bias tool.ResultsEleven studies, with a total of 800 participants with a mean age of 55.51 ± 6.88 years, were identified. All RCTs were performed from 2013 to 2024. There was no statistically significant difference between mHealth-supported exercise compared with the traditional exercise without mHealth in terms of pain reduction (standard mean differences [SMD] = −0.35; 95%CI: −0.74 to 0.04, P = 0.08), functional disability (SMD = −0.5; 95%CI: −0.1 to 0.01; P = 0.05), and quality of life (SMD = 0.11; 95%CI: −0.26 to 0.48; P = 0.56). However, a statistically significant difference was found between mHealth-supported exercise compared with unsupervised traditional exercise in terms of pain (SMD = −1.03; 95%CI: −1.49 to −0.57; P &lt; 0.001) and functional disability (SMD = −0.89; 95%CI: −1.71 to −0.06; P = 0.04).ConclusionmHealth-based exercise was found to be more effective than unsupervised conventional exercise in promoting pain relief and enhancing functional disability in patients with OA. When face-to-face exercise intervention is not feasible, mHealth-based exercise should be considered a viable option in the recovery process for knee OA. Given the significant heterogeneity observed in this study, it is important to exercise caution when extrapolating the results.Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42024610393.
The contributions of language and inhibitory control to false belief reasoning over time
Authors: de Villiers Jill G. , de Villiers Peter 
Published: January 2025

IntroductionThe role of language in false belief reasoning has been much debated for twenty-five years or more, especially the relative contributions of general language development, complement syntax, vocabulary, and executive function. However, the empirical studies so far have fallen short, in that they generally have too few participants for adequate statistical modeling; they do not include control variables; or they are cross-sectional rather than longitudinal, making inferences about causal direction much more tenuous.MethodsThe present study considers the role of these different variables in the development of false belief reasoning over several months of testing, with 258 children aged three to five years. The children are also from under-resourced communities, broadening the populations that generally contribute such data.ResultsA cross-sectional and a longitudinal regression analysis reveals the contribution of each variable to the children’s success on the false belief measures. Finally, a structural equation model tests the relative contribution of the different potential factors over time, how they interact, and change. The model is an excellent fit to the data. Inhibitory control, complement comprehension and vocabulary all have effects on false belief reasoning at the first time point (T1). However, at T3, the major proximal contribution is the child’s comprehension of complements, though the longitudinal pathways of vocabulary and inhibitory control also pave the way.DiscussionOur data confirm the specific contribution of complement syntax but also makes clear, as do training studies, that a certain amount of preparedness in vocabulary and in executive function skills is also necessary.
Do delayed responses introduce bias in ecological momentary assessment? Evidence from comparisons between self-reported and objective physical activity
Authors: Schneider Stefan , Toledo Meynard J. , Junghaenel Doerte U. , Smyth Joshua M. , Lee Pey-Jiuan , Goldstein Sarah , Pomeroy Olivia , Stone Arthur A. 
Published: January 2025

IntroductionDelayed responses are a common yet often overlooked aspect of participant compliance in ecological momentary assessment (EMA) research. This study investigated whether response delays introduce selection bias in the moments captured by EMA.MethodsParticipants (n = 339) self-reported their physical activity behaviors using EMA five times a day over 7 days while wearing a continuous physical activity monitor. The continuous activity monitor data provided an objective reference value to evaluate potential biases in delayed EMA self-reports.ResultsResults showed that participants were significantly more likely to delay EMA responses when they were prompted during higher levels of physical activity, and they subsequently reduced their activity levels, postponing their response until they were in a significantly less active state. There was no significant evidence that response delays systematically biased the levels of EMA reported activities, although delayed responses were associated with significantly more random errors in EMA reports (with small effect sizes).DiscussionThe results suggest that respondents self-select the moments for answering EMA surveys based on their current activity levels, but brief response delays do not translate into marked reductions in the quality of EMA data.
Correlates of mental health of people living with HIV co-infected with SARS-CoV-2: findings from the COVIDHIV study in France
Authors: Yaya Issifou , Amboise Yvenie , Roucoux Guillaume , Yombo-Kokule Lisa , Marcellin Fabienne , Duvivier Claudine , Lacombe Karine , Griffith James W. , Préau Marie , Cheret Antoine , Duracinsky Martin 
Published: January 2025

BackgroundThe COVID-19 pandemic has severely affected vulnerable populations, especially individuals living with HIV/AIDS (PLWHA). The convergence of HIV/AIDS and COVID-19 presents unique challenges, exacerbating existing health concerns and magnifying the strain on individuals already grappling with compromised immune systems. This study aimed to investigate the mental well-being repercussions faced by PLWHA co-infected with SARS-CoV-2 in France.MethodsCOVIDHIV is a French multicenter cohort of PLWHA co-infected with SARS-CoV-2, which collected sociodemographic, clinical, and mental health data. Anxiety and depression symptoms and post-traumatic stress disorder (PTSD) were assessed by the Hospital Anxiety and Depression Scale (HADS) and the PTSD Checklist (PCL-S), respectively. Multivariable logistic regression was performed to identify factors associated with mental health outcomes at inclusion in the cohort.ResultsOf the 397 participants included, 64.7% were male. The mean age was 51.6 (± 11.8) years. The prevalence of mental health outcomes was 33.5% ([95%CI: 28.5-39.0%]) for anxiety, 21.0% ([16.8-25.9%]) for depression, and 12.2% ([8.9-16.5%]) for PTSD. In multivariable regression adjusted for sex, COVID-19 wave and duration between COVID-19 confirmation and enrolment, age (adjusted odds-ratio (aOR): 0.97 [0.95-0.99]), being professionally active (0.43 [0.25-0.75]), and the number of self-reported symptoms (1.17 [1.11-1.24]) were associated with anxiety. Being professionally active (0.34 [0.18-0.65]), living in a couple (0.52 [0.20-0.98]), number of self-reported symptoms (1.15 [1.08-1.22]), and hospitalization for COVID-19 (3.35 [1.34-8.33]) were associated with depression. The number of self-reported symptoms (1.27 [1.16-1.41]), psychiatric disorders (4.04 [1.48-11.11]), and perceived vulnerability to COVID-19 (4.53 [1.69-14.60]) were associated with PTSD.ConclusionThe mental health is a challenging issue among the participants and needs to be closely monitored among people already affected by a chronic disease such as HIV. The findings underscore the urgent need for targeted support and interventions tailored to address the mental health needs of PLWHA facing the dual burden of HIV/AIDS and COVID-19.
Internet-delivered emotional awareness and expression therapy for somatic symptom disorder: one year follow-up
Authors: Hallberg Henrik , Maroti Daniel , Lumley Mark A. , Johansson Robert 
Published: January 2025

ObjectiveWe examined whether the treatment effects from a previous RCT of Internet-delivered Emotional Awareness and Expression Therapy (I-EAET) for somatic symptom disorder were maintained 12 months after treatment.Method12-month assessments of self-reported somatic symptoms, pain severity, and several secondary outcomes were compared with baseline and post-treatment levels within the I-EAET condition only, given that the waitlist control condition had already received treatment. Twenty-eight out of the original 37 participants (76%) in the I-EAET condition provided follow-up data.ResultsThe beneficial effects of I-EAET on somatic symptoms observed at post-treatment were maintained at the 12-month follow-up (d = -0.22, 95% CI: -0.72 to 0.28), as well as for pain intensity (d = -0.02, 95% CI: -0.52 to 0.48). From pre-treatment to 12-month follow-up, there was a medium effect on somatic symptoms (d = 0.74, 95% CI 0.23 to 1.24), and a small, non-significant effect for pain intensity (d = 0.43, 95% CI -0.06 to 0.93). Response rates (at least 50% symptom reduction) at 12-month follow-up were 25% for somatic symptoms, and 12% for pain intensity.ConclusionI-EAET seems to have positive long-term effects for somatic symptom disorder. Larger studies with controls and comparisons to other treatments are needed.
Humanizing processes after harm part 1: patient safety incident investigations, litigation and the experiences of those affected
Authors: Ramsey Lauren , Sheard Laura , Waring Justin , McHugh Siobhan , Simms-Ellis Ruth , Louch Gemma , Ludwin Katherine , O’Hara Jane K. 
Published: January 2025

BackgroundThere is a growing international policy focus on involving those affected by healthcare safety incidents, in subsequent investigations. Nonetheless, there remains little UK-based evidence exploring how this relates to the experiences of those affected over time, including the factors influencing decisions to litigate.AimsWe aimed to explore the experiences of patients, families, staff and legal representatives affected by safety incidents over time, and the factors influencing decisions to litigate.MethodsParticipants were purposively recruited via (i) communication from four NHS hospital Trusts or an independent national investigator in England, (ii) relevant charitable organizations, (iii) social media, and (iv) word of mouth to take part in a qualitative semi-structured interview study. Data were analyzed using an inductive reflexive thematic approach.Findings42 people with personal or professional experience of safety incident investigations participated, comprising patients and families (n = 18), healthcare staff (n = 7), legal staff (n = 1), and investigators (n = 16). Patients and families started investigation processes with cautious hope, but over time, came to realize that they lacked power, knowledge, and support to navigate the system, made clear in awaited investigation reports. Systemic fear of litigation not only failed to meet the needs of those affected, but also inadvertently led to some pursuing litigation. Staff had parallel experiences of exclusion, lacking support and feeling left with an incomplete narrative. Importantly, investigating was often perceived as a lonely, invisible and undervalued role involving skilled “work” with limited training, resources, and infrastructure. Ultimately, elusive “organizational agendas” were prioritized above the needs of all affected.ConclusionsIncident investigations fail to acknowledge and address emotional distress experienced by all affected, resulting in compounded harm. To address this, we propose five key recommendations, to: (1) prioritize the needs of those affected by incidents, (2) overcome culturally engrained fears of litigation to re-humanize processes and reduce rates of unnecessary litigation, (3) recognize and value the emotionally laborious and skilled work of investigators (4) inform and support those affected, (5) proceed in ways that recognize and seek to reduce social inequities.
Access improvement in healthcare: a 12-step framework for operational practice
Authors: Chen Allen M. 
Published: January 2025

BackgroundAccess improvement is a fundamental component of value-based healthcare as it inherently promotes quality by eliminating chokepoints, redundancies, and inefficiencies which could hinder the provisioning of timely care. The purpose of this review is to present a 12-step framework which offers healthcare organizations a practical, thematic-based foundation for thinking about access improvement.MethodsThis study was designed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) statement. A literature search of prospective peer-reviewed publications was undertaken to identify studies pertaining to healthcare access. Articles published from January 2014 to January 2024 were included. An interpretive synthesis was then presented.ResultsA total of 469 peer-reviewed studies were identified. The most common diseases analyzed were related to general medicine/family practice (N = 75), surgical care (N = 51), health screening (N = 30), mental health (N = 27), cardiovascular disease (N = 17), emergency room/critical care (N = 15), and cancer (N = 7). The remaining 247 studies (53%) did not specifically report on any specialization. The core themes could be broadly categorized into the following: workforce adequacy, patient experience, physical space utilization, template optimization, scheduling efficiency, process standardization, cost transparency, physician engagement, and data analytics. Sixty publications (13%) focused at least in part on equity issues, structural racism, and/or implicit bias; and 25 publications (5%) addressed disparities in education, training, and/or technical literacy. Seventy-three publications (16%) focused either completely or in part on digital health as a means of access improvement.ConclusionBased on this systematic review, a 12-step thematically based framework for approaching access improvement in healthcare was developed.
The effects of sex and load on quantifying the bilateral force deficit during an upper body Wingate test
Authors: Antolinez Angie K. , Edwards Philip F. , Holmes Michael W. R. , Button Duane C. 
Published: January 2025

IntroductionThe bilateral deficit (BLD) is a reduction in the amount of force during a bilateral task vs. the total force from the unilateral limbs performing the same task. We quantified the BLD during an upper body Wingate Anaerobic Test (WAnT) and evaluated the influence of sex and load on the BLD in force.MethodsEighteen participants performed maximum handgrip strength, voluntary isometric contractions (MVIC), and three 30s WAnTs. In each session they completed the tasks with the dominant-arm, non-dominant arm and with both arms, randomly. WAnT intensities were 3, 4, and 5% body weight (BW). Instantaneous force data was used to calculate the BLD.ResultsMales showed greater (p &lt; .001) BLD of force at 3, 4, and 5% BW than females by −17, −27.6 and −36%, respectively and had a greater (p &lt; .001) BLD of force than females throughout time points 1–10 s, 11–20 s, and 21–30 s by −16, −29 and −35%, respectively. Females showed a difference (p &lt; .001) in BLD of force between loads (−19% at 3%, −10% at 4% and +7% at 5%). Males had an increase (p &lt; .001) in BLD of force from the beginning to the end of the WAnT starting with −18% (1–10 s), −38% (11–20 s) and −40% (21–30 s). WAnT had the highest BLD, followed by MVIC and grip strength.DiscussionBLD in force is present during WAnTs and the sex-load interaction is important for determining this BLD during this maximal cycling test. Thus, when developing training or rehabilitation programs related to BLD in force, sex, load and exercise type should be taken into consideration.
Metabolomic profiling and antibacterial efficacy of probiotic-derived cell-free supernatant encapsulated in nanostructured lipid carriers against canine multidrug-resistant bacteria
Authors: Myo Nay Zin , Kamwa Ratchnida , Jamnong Thitirat , Swasdipisal Busaba , Somrak Papavarin , Rattanamalakorn Phanchompoo , Neatsawang Vipada , Apiwatsiri Prasert , Yata Teerapong , Hampson David J. , Prapasarakul Nuvee 
Published: January 2025

AimThis study aimed to investigate the antibacterial efficacy of probiotic-derived cell-free supernatants (CFS) encapsulated within nanostructured lipid carriers (NLCs) against multidrug-resistant Pseudomonas aeruginosa and Staphylococcus pseudintermedius. Additionally, it aimed to identify specific bioactive compounds that contribute to the reported antibacterial properties by characterizing the metabolite substances present in the CFS using a metabolomic analysis technique.MethodsEight strains of lactic acid bacteria including Lactiplantibacillus plantarum (L22F and L25F), Pediococcus acidilactici (P72N, BF9, BF 14, BYF 20 and BYF 26) and Ligilactobacillus salivarius (BF 12) were selected as probiotic candidates. The inhibitory activity of their cell free supernatant (CFS) was tested against clinical strains of P. aeruginosa and S. pseudintermedius isolated from skin wounds of dogs and cats. An untargeted metabolomic approach based on liquid chromatography-mass spectrometry (LC-MS) identified potential antibacterial metabolites in the CFS. Cell-Free Supernatants-Nanostructured Lipid Carriers (CFS-NLCs) were developed, and their antibacterial activity and minimum bactericidal concentration (MBC) were analysed.ResultsDespite the strong multidrug-resistant nature of the pathogens, CFS displayed a moderate antibacterial activity against most tested strains. The acidic nature of the CFS, combined with bioactive antibacterial metabolites like Kanzonol V and 1-Hexanol, likely contributed to its inhibitory effects against pathogenic bacteria; notably, Kanzonol V was abundant in the CFS of L22F, BF12 and BYF26 (L22F_CFS, BF12_CFS and BYF26_CFS), while 1-Hexanol was particularly enriched in CFS of P72N (P72N_CFS), with both compounds effectively targeting bacterial cell membranes to disrupt cell integrity, leading to bacterial cell death. Other beneficial compounds such as Pyroglutamylleucine, Trigoneoside VIII and 18-Nor-4(19),8,11,13-abietatetraene which are likely to have anti-inflammatory, antimicrobial and antioxidant activities, were also detected in the CFS. The CFS-NLCs maintained their antibacterial activity and 30–60% dilutions of product completely inhibited the growth of pathogen strains even after three-months storage at room temperature.ConclusionThese findings suggest that CFS-NLCs could be a promising biotic therapy for treating hospital infections such as canine dermatitis and otitis caused by multidrug-resistant P. aeruginosa and S. pseudintermedius.