bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteopontin induces osteogenic differentiation by human periodontal ligament cells via calcium binding domain–ALK‐1 interaction
Authors: Chaikiawkeaw Daneeya , Khorattanakulchai Narach , Nammultriputtar Ketsaraporn , Rattanapisit Kaewta , Everts Vincent , Kubera Anchanee , Phoolcharoen Waranyoo , Pavasant Prasit 
Published: August 2021

AbstractBackgroundRecently we have generated recombinant human osteopontin (rhOPN) using a plant platform (Nicotiana benthamiana) and demonstrated, when coated on culture plates, its osteogenic induction capacity of human periodontal ligament (PDL) cells. The aim of this study is to elucidate the molecular mechanism underlying the rhOPN‐induced osteogenic differentiation of human PDL cells.MethodsFull length rhOPN (FL‐OPN) and three constructs of OPN containing integrin binding domain (N142), calcium binding domain (C122) and mutated calcium‐binding domain (C122δ) were generated from N. benthamiana. Human PDL cells were isolated from extracted third molars and cultured on FL‐OPN, N142, C122, or C122δ‐coated surfaces. Real‐time PCR and Western blot analyses were used to determine mRNA and protein expression. In vitro calcification was determined by Alizarin red staining. A chemical inhibitor and RNAi silencing were used to elucidate signaling pathways. In silico analyses were performed to predict the protein‐protein interaction. In vivo analysis was performed using a rat calvaria defect model.ResultsHuman PDL cells seeded on FL‐OPN and C122‐coated surfaces significantly increased both mRNA and protein expression of osterix (OSX) and enhanced in vitro calcification. Soluble FL‐OPN as well as a surface coated with N142 did not affect OSX expression. Inhibition of activin receptor‐like kinase (ALK‐1) abolished the induction of osterix expression. In silico analysis suggested a possible interaction between the calcium binding domain (CaBD) of OPN and ALK‐1 receptor. C122, but not C122δ coated surfaces, induced the expression of p‐Smad‐1 and this induction was inhibited by an ALK‐1 inhibitor and RNAi against ALK‐1. In vivo data showed that 3D porous scaffold containing C‐122 enhanced new bone formation as compared to scaffold alone.ConclusionThe results suggest that next to full length OPN, the CaBD of OPN, if coated to a surface, induces osteogenic differentiation via interaction with ALK‐1 receptor.
Impact of interproximal composite restorations on periodontal tissue health: Clinical and cytokine profiles from a pre‐post quasi‐experimental study
Authors: Discepoli Nicola , Marruganti Crystal , Santoro Francesco , Mirra Raffaele , Fiorino Fabio , Medaglini Donata , Pozzi Gianni , Ferrari Marco , Grandini Simone 
Published: August 2021

AbstractBackgroundThe aim of this study is to clinically and molecularly evaluate the effect of an interproximal iuxta/subgingival direct composite restoration on periodontal tissue healing.MethodsIndividuals in need of a posterior iuxta/subgingival interproximal restoration were consecutively enrolled. After enrollment, a test (site with tooth decay) and a control site (intact contralateral tooth) were identified. After a periodontal examination (probing depth [PD], clinical attachment level, recession, plaque, and bleeding on probing [BOP]) and a sampling of gingival crevicular fluid, the composite restoration was performed (T0). Clinical and molecular assessments were repeated at 3 (T3), 6 (T6), and 12 (T12) months after the restoration. Intragroup pre‐post comparisons for quantitative variables were performed either through one‐way ANOVA or Kruskal‒Wallis test. A multivariate linear regression analysis was then modeled. With α = 0.05, a power of 80% will be reached with the inclusion of 41 individuals.ResultsBiometric parameters demonstrated an increased mean PD (ΔPDT0‐T12 = ‐0.83 mm; P = 0.001) and loss of attachment (AL) (ΔCALT0‐T12 = ‐0.91 mm; P = 0.005) in the test site at 12 months. Accordingly, in the final multivariate regression model the radiographic distance between the bone crest and the restorative margin at baseline accounted for the dependent variable “attachment loss (AL)” (ΔCALT0‐T12) (P <0.05).ConclusionsIuxta/subgingival interproximal restorative margins jeopardized clinically and molecularly the periodontal tissue healing at least up to 1 year of follow‐up.
Phosphorus mobilization and improvement of crop agronomic performances by a new white‐rot fungus Ceriporia lacerataHG2011
Authors: Sui Zongming , Yin Jie , Huang Jianguo , Yuan Ling 
Published: August 2021

AbstractBACKGROUNDSome soil microorganisms can mobilize unavailable phosphorus (P) in soils for plant use and increase P fertilizer efficiency. Thus, an abiotic P solubilization experiment and fungal incubation in solution and soil were conducted to investigate the mobilization of various P compounds by a new white‐rot fungus Ceriporia lacerata HG2011. The crop agronomic performances were then evaluated in the winter barley–summer maize–winter wheat rotation field.RESULTSCeriporia lacerata HG2011 had a wide P mobilization spectrum and mobilized P by different mechanisms depending on P sources supplied in liquid culture. The chief mechanism employed by this fungus was the production of protons in mobilizing Ca3(PO4)2, low‐molecular‐weight organic acids and other unknown substances in FePO4 and AlPO4, phytase (an inducible enzyme in the presence of phytate) in phytate, and phosphatase in lecithin and ribonucleic acid, respectively. As a result of the large fungal biomass, P accumulated in the hypha should also be considered in the assessment of the fungal P mobilization, and not just only soluble inorganic P. As C. lacerata HG2011 colonized on and in the test soil, phosphatase and phytase activities were enhanced but pH decreased in the soil, leading to P mobilization. The application of this fungus mobilized soil P, increased crop P uptake and yields, and consecutively reduced P fertilizer use without yield sacrifices in the multiple crop rotation field.CONCLUSIONC. lacerata HG2011 showed a new use with respect to mobilizing soil P and reducing P fertilizer input in modern agriculture beyond medical purposes, environmental protection and biofuel production. © 2021 Society of Chemical Industry.
Legumes as basic ingredients in the production of dairy‐free cheese alternatives: a review
Authors: Mefleh Marina , Pasqualone Antonella , Caponio Francesco , Faccia Michele 
Published: August 2021

AbstractResearch into dairy‐free alternative products, whether plant‐based or cell‐based, is growing fast and the food industry is facing a new challenge of creating innovative, nutritious, accessible, and natural dairy‐free cheese alternatives. The market demand for these products is continuing to increase owing to more people choosing to reduce or eliminate meat and dairy products from their diet for health, environmental sustainability, and/or ethical reasons. This review investigates the current status of dairy product alternatives. Legume proteins have good technological properties and are cheap, which gives them a strong commercial potential to be used in plant‐based cheese‐like products. However, few legume proteins have been explored in the formulation, development, and manufacture of a fully dairy‐free cheese because of their undesirable properties: heat stable anti‐nutritional factors and a beany flavor. These can be alleviated by novel or traditional and economical techniques. The improvement and diversification of the formulation of legume‐based cheese alternatives is strongly suggested as a low‐cost step towards more sustainable food chains. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Eleocharis dulcis corm: phytochemicals, health benefits, processing and food products
Authors: Zhang Yang , Xu Hai , Hu Zhenbiao , Yang Guihong , Yu Xiaojin , Chen Qianfeng , Zheng Lixue , Yan Zhaowei 
Published: August 2021

AbstractEleocharis dulcis, an aquatic plant belonging to Cyperaceae family, is indigenous to Asia, and also occurs in tropical Africa and Australia. The edible corm part of E. dulcis is a commonly consumed aquatic vegetable with a planting area of 44.46 × 103 hm2 in China. This work aims to explore the potential of E. dulcis corm for use as a new food source for sufficient nutrients and health benefits by reviewing its nutrients, phytochemicals, functions, processing and food products. Eleocharis dulcis corm contains starches, dietary fibers, non‐starch polysaccharides, proteins, amino acids, phenolics, sterols, puchiin, saponins, minerals and vitamins. Among them, phenolics including flavonoids and quinones could be the major bioconstituents that largely contribute to antioxidant, anti‐inflammatory, antibacterial, antitumor, hepatoprotective, neuroprotective and hypolipidemic functions. Peel wastes of E. dulcis corm tend to be enriched in phenolics to a much higher extent than the edible pulp. Fresh‐cut E. dulcis corm can be consumed as a ready‐to‐eat food or processed into juice for beverage production, and anti‐browning processing is a key to prolonging shelf life. Present food products of E. dulcis corm are centered on various fruit and vegetable beverages, and suffer from single categories and inadequate development. In brief, underutilized E. dulcis corm possesses great potential for use as a new food source for sufficient nutrients and health benefits. © 2021 Society of Chemical Industry.
Integral imaging 3D display based on dual time multiplexing with liquid crystal aperture array
Authors: Zhao Yulu , Zhang Xinran , Liao Hongen 
Published: August 2021

AbstractDue to the limitations of the lens array of the integral imaging display, the viewing angle and resolution are mutually restricted in space, and are difficult to improve at the same time. We propose an integral imaging system based on dual time division multiplexing using a liquid crystal aperture array to improve viewing angle and resolution simultaneously. A liquid crystal panel is introduced into the traditional integral imaging display system as an adjustable aperture array, which is used to change the imaging effect of the micro‐lens array and to realize the dual time division multiplexing of elemental image pixels. The liquid crystal aperture array is controlled to gate different lenses and different positions on the lens at different times, then the corresponding elemental image array is rendered. Thus, the spatial constraints of viewing angle and resolution are transformed into the time domains by multiplexing. Based on the proposed scheme, this paper also proposes a synchronous rendering algorithm for elemental image array and aperture array patterns in the different multiplexing periods. To verify the feasibility of the scheme, we built a prototype display and the experimental results verified that our method achieved certain improvements in viewing angle and resolution.
Solid‐phase extraction methods for nucleic acid separation. A review
Authors: Li Peipei , Li Menghang , Yue Dongmei , Chen Haixia 
Published: August 2021

AbstractThe separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.
Simultaneous determination of 11 quinolone residues in freshwater fish samples by magnetic solid‐extraction coupled to liquid chromatography‐tandem mass spectrometry
Authors: Pan Shengdong , Xu Qihong , Guo Yanbo , Wang Li 
Published: August 2021

AbstractIn this study, well‐defined core‐shell ethylenediamine‐functional magnetic ferroferric oxide polymers were prepared and were fully characterized by transmission electron microscopy, scanning electron microscopy, FTIR spectroscopy, and vibrating sample magnetometry. Then, it was used as a magnetic solid‐phase extraction adsorbent for simultaneous determination of 11 trace quinolone residues in freshwater fish samples coupled to liquid chromatography‐tandem mass spectrometry. The obtained results revealed that the adsorbent showed good extraction efficiency and the adsorption mechanisms referred to hydrogen bond and π‐π stacking interaction. Moreover, the magnetic solid‐phase extraction conditions were also carefully optimized. The limits of quantitation of 11 quinolones were in the range of 0.15–0.36 μg/kg, while spiking recoveries were in the range of 80.2–99.5% for the 11 quinolones in freshwater fish samples at four spiked levels including limits of quantitation, 1.0, 40.0, and 80.0 μg/kg with the relative standard deviations ranging from 0.8 to 9.1%. The proposed method was applied to analyze 45 freshwater fish samples, and enrofloxacin was detected in 91.1% samples with concentrations ranging from 0.659 to 333 μg/kg. It could be concluded that the proposed method is fast, simple, sensitive, and accurate for the routine monitor of freshwater fish.
Safety of sitagliptin in treatment of hepatocellular carcinoma in chronic liver disease patients
Authors: Hollande Clémence , Boussier Jeremy , Mottez Estelle , Bondet Vincent , Buivan Tan Phuc , Charbit Bruno , Llibre Alba , Charlotte Frédéric , Savier Eric , Scatton Olivier , Duffy Darragh , Albert Matthew , Mallet Vincent , Pol Stanislas 
Published: August 2021

AbstractBackground & AimsSystemic therapies for hepatocellular carcinoma (HCC) treatment have limited efficacy and poor safety. Dipeptidyl peptidase‐4 inhibitors were initially developed and approved as treatment for type 2 diabetes, yet oral administration of sitagliptin has recently been shown to improve naturally occurring tumour immunity in animal models of HCC.MethodsWe conducted a phase Ib clinical trial to evaluate the impact of a pre‐operative 3‐week DPP4 inhibitor (sitagliptin) treatment in HCC patients undergoing liver resection. The primary objective was to evaluate the safety of a sitagliptin treatment in each of the three groups of patients, according to an escalating dosage of sitagliptin (100, 200 and 600 mg/d). Secondary objectives included the assessment of DPP4 activity, cytokine expression in plasma samples and circulating immune populations.ResultsFourteen patients were included and analysed. In all three dose groups, no severe adverse event related to sitagliptin was reported. A significant inhibition of DPP4 activity was observed upon sitagliptin treatment, which prevented the N‐terminal truncation of CXCL10, leading to a mobilization of circulating CD8+ T cells and eosinophils. Immunochemistry analysis showed a lymphoid infiltration in all tumour samples with the presence of a population of CXCR3+ T cells in all but one of the tumours. Positivity for CXCL10 (IP10) and CCR3 in tumour and/or stroma cells was found in all resection pieces.ConclusionIn summary, sitagliptin can be used safely in patients with chronic liver disease and HCC, and could be tested in phase 2 trial, as an adjuvant in combination with others drugs, for the treatment of HCC patients.
Homeostasis drives intense microbial trace metal processing on marine particles
Authors: Debeljak Pavla , Blain Stéphane , Bowie Andrew , van der Merwe Pier , Bayer Barbara , Obernosterer Ingrid 
Published: August 2021

AbstractAs marine microorganisms and environmental conditions coevolved over geological timescales, metals have been incorporated into all essential metabolic processes. In the modern ocean, metals are present from trace amounts limiting microbial growth to toxic concentrations. Dissolved trace metals are a major bioavailable reservoir. However, the acquisition of metals from marine particles remains largely unexplored. Here, we combined chemical characterization and a comparative metatranscriptomics approach to investigate the availability of nine metals of biological importance on particles collected in the region of Heard Island (Indian sector of the Southern Ocean). Elemental ratios identified particulate matter as a potential source of metals for prokaryotes. The expression of genes for the uptake of metals through various mechanisms demonstrated that particles are a bioavailable reservoir. But genes involved in the control of resistance to metal toxicity, storage, sensing, and regulation were also highly expressed. Our observations suggest that homeostasis associated with a diverse prokaryotic community is the overarching mechanism that enhances the trace element processing on particles. These results provide clues that microbial activity on particles is critical in the redistribution of trace elements between different fractions and chemical forms in the ocean.
Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor
Authors: Okubo Takashi , Takami Hideto 
Published: August 2021

AbstractThe imperfect denitrifier, Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential of Ca. D. denitrificans and deduce its functional relationships to anammox bacteria (i.e., Ca. Brocadia pituitae). Although Ca. D. denitrificans is hypothesized to supply NO to Ca. B. pituitae as a byproduct of imperfect denitrification, this microbe also possesses hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to NO and potentially the reverse reaction. Ca. D. denitrificans can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen, but metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. The interrelationship between Ca. D. denitirificans and Ca. B. pituitae via the metabolism of aromatic compounds may govern the population balance of both species. Ca. D. denitrificans also has the potential to fix CO2 via an irregular Calvin cycle and couple denitrification to the oxidation of hydrogen and sulfur compounds under chemolithoautotrophic conditions. This metabolic versatility, which suggests a mixotrophic lifestyle, would facilitate the growth of Ca. D. denitrificans in the anammox bioreactor.
Expedition of sulfur‐containing heterocyclic derivatives as cytotoxic agents in medicinal chemistry: A decade update
Authors: Laxmikeshav Kritika , Kumari Pooja , Shankaraiah Nagula 
Published: August 2021

AbstractThis review article proposes a comprehensive report of the design strategies engaged in the development of various sulfur‐bearing cytotoxic agents. The outcomes of various studies depict that the sulfur heterocyclic framework is a fundamental structure in diverse synthetic analogs representing a myriad scope of therapeutic activities. A number of five‐, six‐ and seven‐membered sulfur‐containing heterocyclic scaffolds, such as thiazoles, thiadiazoles, thiazolidinediones, thiophenes, thiopyrans, benzothiazoles, benzothiophenes, thienopyrimidines, simple and modified phenothiazines, and thiazepines have been discussed. The subsequent studies of the derivatives unveiled their cytotoxic effects through multiple mechanisms (viz. inhibition of tyrosine kinases, topoisomerase I and II, tubulin, COX, DNA synthesis, and PI3K/Akt and Raf/MEK/ERK signaling pathways), and several others. Thus, our concise illustration explains the design strategy and anticancer potential of these five‐ and six‐membered sulfur‐containing heterocyclic molecules along with a brief outline on seven‐membered sulfur heterocycles. The thorough assessment of antiproliferative activities with the reference drug allows a proficient assessment of the structure–activity relationships (SARs) of the diversely synthesized molecules of the series.
Is Galactose a Hormetic Sugar? An Exploratory Study of the Rat Hippocampal Redox Regulatory Network
Authors: Homolak Jan , Babic Perhoc Ana , Knezovic Ana , Kodvanj Ivan , Virag Davor , Osmanovic Barilar Jelena , Riederer Peter , Salkovic‐Petrisic Melita 
Published: August 2021

ScopeGalactose, a ubiquitous monosaccharide with incompletely understood physiology is often exploited for inducing oxidative‐stress mediated aging in animals. Recent research demonstrates that galactose can conserve cellular function during periods of starvation and prevent/alleviate cognitive deficits in a rat model of sporadic Alzheimer's disease. The present aim is to examine the acute effects of oral galactose on the redox regulatory network (RRN).Methods and ResultsRat plasma and hippocampal RRNs are analyzed upon acute orogastric gavage of galactose (200 mg kg‒1). No systemic RRN disbalance is observed; however, a mild pro‐oxidative shift accompanied by a paradoxical increment in tissue reductive capacity suggesting overcompensation of endogenous antioxidant systems is observed in the hippocampus. Galactose‐induced increment of reductive capacity is accompanied by inflation of the hippocampal pool of nicotinamide adenine dinucleotide phosphates indicating ROS detoxification through disinhibition of the oxidative pentose phosphate pathway flux, reduced neuronal activity, and upregulation of Leloir pathway gatekeeper enzyme galactokinase‐1.ConclusionBased on the observed findings, and in the context of previous work on galactose, a hormetic hypothesis of galactose is proposed suggesting that the protective effects may be inseparable from its pro‐oxidative action at the biochemical level.