bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of an autophagy-related gene marker for predicting the prognosis in lung adenocarcinoma patients
Authors: wang wenhao , Yang Longjun , Lu Zhong , Sun Xiumei , Liu Jin , Sheng Zhimei , zhang baogang 
Published: August 2021

Abstract
BackgroundLung adenocarcinoma (LUAD) is a tumor with high incidence rate and high mortality rate. Previous studies have found that autophagy plays a vital role in tumorigenesis and biological progression. The aim of our research is to screen and analyze autophagy-related genes (ATGs) using bioinformatics technology, then establish a gene expression model for predicting the prognosis of LUAD patients. MethodsDifferentially expressed ATGs in LUAD and normal tissues were screened by The Cancer Genome Atlas (TCGA) dataset. We applied Go and KEGG enrichment analysis of ATGs for identifying the relevant signaling pathways. Finally, the ATGs related with survival were assessed using univariate and multivariate COX regression analyses. This project was analyzed by R software. ResultsA total of 232 ATGs were obtained in LUAD. Subsequently, 30 ATGs were screened out as genes associated with prognosis. GO analysis revealed that the 30 differently expressed ATGs (DE-ATGs) were enriched in intrinsic apoptotic signaling pathway, macroautophagy, and neuron death. In addition, KEGG analysis revealed that the DE-ATGs were associated with autophagy (animal), ErbB signaling pathway and IL-17 signaling pathway. Then, the risk score model was established by the 8 ATGs (ATG4A, CCR2, MBTPS2, APOL1, ERO1A, SPHK1, ST13 and ITGA6), and LUAD patients were divided into high-risk and low-risk groups with the risk score. The risk score was significantly related to overall survival and prognosis by univariate and multivariate analysis (P < 0.001). The survival time of low-risk score patients was showed by the cumulative curve that was obviously longer than high-risk score patients (P< 0.001). Finally, the correlation of the autophagy-related risk characteristics and multiple clinical parameters was analyzed. ConclusionA prognostic signature and nomogram based on 8 ATGs was constructed. This research provides a new direction for the prognosis evaluation and guides the potential treatment strategies for LUAD.
Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7
Authors: Li Xiangfei , Han Rumeng , Bao Teng , Osire Tolbert , Zhang Xian , Xu Meijuan , Yang Taowei , Rao Zhiming 
Published: August 2021

Abstract
Background

Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability.
Results

This study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intracellular ATP and the biomass by 80.5% and 31.6%, respectively, resulting in a 18.6% and 60.3%, increased in ethanol and the alcohol/acid production ratio, respectively.
Conclusions

This is the first report that citrulline could promote the growth of C. carboxidivorans P7 and increased the level of intracellular ATP, which is of great significance for the use of C. carboxidivorans P7 to synthesize biofuels.
Impact of Substrate Temperature on Structural, electric and Optical characteristics of CuO Thin Films grown by JNS Pyrolysis Technique
Authors: N Jhansi , D Balasubramanian , Chang Jih-Hsing , Kumar Mohanraj , Raj Marnadu , M Aslam Manthrammel , Shkir Mohd. 
Published: August 2021

Abstract
JNS pyrolysis route has been successfully employed to grow CuO thin films at various substrate temperature, ranging from 300 to 600˚C. The XRD analyses revealed the monoclinic phased polycrystalline growth of the samples and exhibited the strong influence of the substrate temperature (ST) on the crystallite sizes. Optical transmission and bandgap studies also showed that sample bandgaps clearly rely upon the growth temperatures. The SEM micrographs displayed the agglomerated growth of particles having golf ball-like structures. The occurrence of Cu and O in the samples were confirmed through EDS analyses. The studies on DC electrical conductivities also shows strong dependency on the ST. A p-CuO/n-Si diode was fabricated at the ST of 600˚C and the diode parameters like barrier height ( ϕb ) and ideality factor (n) were determined under light and dark conditions.
Prevalence of Anemia and Associated Factors Among Pregnant Women Attending Antenatal Care Service in Worabe Comprehensive Specialized Hospital, Silte Zone, SNNPR State, Ethiopia
Authors: Mohammed Neja Awol 
Published: August 2021

Abstract
ObjectiveThe aim of this study was to determine the prevalence ofanaemia and associated factor among pregnant women attending ANC service in Worabe Comprehensive Specialized Hospital from September to December 2016, Silte ZoneSouthern Ethiopia.MethodA Cross-sectional study was conducted from September to December 2016.on pregnant women. Demographic and other required data that has association contributing for the occurrence of anaemia in pregnant women was collected using pre structured questionnaires, & haemoglobin were measured using Mindray CD-3200 Machine. The association of data collected with independent variable are analysed by using SPSS and possible conclusions and recommendations was forwarded depending on the findings.
Age differences in adaptation of medial-lateral gait parameters during split-belt treadmill walking
Authors: Fettrow Tyler , Hupfeld Kathleen , Reimann Hendrik , Choi Julia , Hass Chris , Seidler Rachael 
Published: August 2021

Abstract
The split-belt treadmill (SBT) has been used to examine the adaptation of spatial and temporal gait parameters. Historically, SBT studies have focused on anterior-posterior (AP) spatiotemporal gait parameters because SBT is primarily a perturbation in the anterior-posterior direction, but it is important to understand whether and how ML control adapts in this scenario. The medial-lateral (ML) control of balance must be actively controlled and adapted in different walking environments. Furthermore, we seek to determine whether ML balance adaptation differs in older age. We analyzed SBT-induced changes in gait parameters including variables which inform us about ML balance control in younger and older adults. Our primary finding is that younger adults showed sustained asymmetric changes in these ML balance parameters during the split condition of the SBT. Specifically, younger adults sustained a greater displacement between their fast stance foot and their upper body, relative to the slow stance foot, in the ML direction. This finding suggests that younger adults may be exploiting passive dynamics in the ML direction during the SBT, which may be more metabolically efficient. Additionally, the ML parameters did not show any aftereffects despite large adjustments during the split condition, which may indicate higher level neural control than AP parameters.
Pregabalin induces pathological changes in cerebrum and cerebellum of Albino Rats
Authors: Ali Shaimaa , Hassan Wael Abdo 
Published: August 2021

Abstract
Background: Pregabalin (PGB) used as analgesic in remedy of neurogenic pains of persistent diseases, is taken into consideration as one of the maximum abused anti-epileptic pills and it's been proved that it induces addictive behaviors. The current study investigated the impact of PGB management on cerebral cortex and cerebellar cortex, in each acute and chronic toxicity. Seventy- male and non-pregnant female albino rats’ 6- to 8-week-vintage divided into three principal corporations of 24 rats each had been studied. Group 1 was the control group, group 2 represented the acute toxicity group, and group three represented the hronic toxicity group. Rats had been sacrificed and examined. Result: Cerebral cortex tissue of acute toxicity group displayed astrocytosis and dystrophic changes, while the chronic toxicity group showed degeneration, necrosis and inflammatory cell infiltrates. The cerebellum of chronic toxicity group showed affection of Purkinje cells. Conclusion: Acute and chronic intoxication with pregabalin adversely altered the structure and thus the function of the cerebral cortex and cerebellum.
Estudo do perfil epidemiológico dos pacientes portadores de tuberculose na Região de Saúde Centro-Sul (RJ) dos casos confirmados entre 2015 e 2019.
Authors: Franco Borges Renato , Jorge da Cunha Gonçalves Sebastião 
Published: August 2021

Cerca de um terço da população mundial possui infecção pelo Mycobacterium tuberculosis, sendo de 5% a 10% dessas pessoas, ou adoecerão, ou se tornarão infectantes da tuberculose em algum momento de suas vidas. O objetivo do presente estudo é desenhar o perfil epidemiológico com as características mais comum dos pacientes com tuberculose nos últimos 5 anos (2015 a 2019) na Região de Saúde Centro-Sul (RJ), visando a criação de ações para a prevenção da ocorrência de novos casos, diminuindo morbidades e mortalidade, além de diminuir o gasto com tuberculose pelo SUS de forma local. Trata-se de um estudo transversal, observacional, quantitativo, descritivo, onde os dados foram retirados do Sistema de Informação de Agravos de Notificação – Sinan Net, dos anos de 2015 a 2019. Foram notificados 741 casos de tuberculose no período, onde o perfil mais comum encontrado foi de pacientes com escolaridade com ensino médio completo, raça branca, sexo masculino, entrada sendo um caso novo, forma pulmonar, positividade para HIV menor que o índice nacional e com maior percentual de teste, menor índice de realização de tratamento supervisionado e maior índice de encerramento por cura. Existem ações específicas que podem ser implementadas de acordo com cada cidade que compõem a Região de Saúde Centro-Sul (RJ) para melhorar os índices, embora, como visto nos resultados, está superior à média nacional em muitos pontos.
Palavras-chave: Tuberculose; Perfil de saúde; Controle de doenças transmissíveis;
Using the Behaviour Change Wheel Program Planning Model to Design Games for Health: Development Study
Authors: Robertson Michael C , Baranowski Tom , Thompson Debbe , Basen-Engquist Karen M , Swartz Maria Chang , Lyons Elizabeth J 
Published: August 2021

Background
Games for health are a promising approach to health promotion. Their success depends on achieving both experiential (game) and instrumental (health) objectives. There is little to guide game for health (G4H) designers in integrating the science of behavior change with the art of game design.

Objective
The aim of this study is to extend the Behaviour Change Wheel program planning model to develop Challenges for Healthy Aging: Leveraging Limits for Engaging Networked Game-Based Exercise (CHALLENGE), a G4H centered on increasing physical activity in insufficiently active older women.

Methods
We present and apply the G4H Mechanics, Experiences, and Change (MECHA) process, which supplements the Behaviour Change Wheel program planning model. The additional steps are centered on identifying target G4H player experiences and corresponding game mechanics to help game designers integrate design elements and G4H objectives into behavioral interventions.

Results
We identified a target behavior of increasing moderate-intensity walking among insufficiently active older women and key psychosocial determinants of this behavior from self-determination theory (eg, autonomy). We used MECHA to map these constructs to intervention functions (eg, persuasion) and G4H target player experiences (eg, captivation). Next, we identified behavior change techniques (eg, framing or reframing) and specific game mechanics (eg, transforming) to help realize intervention functions and elicit targeted player experiences.

Conclusions
MECHA can help researchers map specific linkages between distal intervention objectives and more proximal game design mechanics in games for health. This can facilitate G4H program planning, evaluation, and clearer scientific communication.
THE REPRESENTATION OF INDONESIAN WOMEN IN CONTEMPORARY TRAVEL WRITING: A STUDY OF DISCOURSE ON GENDER AND TRAVEL WRITING
Authors: Jaya Akmal , Pratama Mochamad Rizqi Adhi 
Published: August 2021

This research aims to examine the representation of Indonesian women in contemporary travel writings. The rise of globalization has challenged the domination of discourses that tend to place Indonesian women in the subordinate position. However, the challenge does not warrant a symmetrical relation between genders, as it sometimes blurs the relation as a result of clashes between discourses. This study, then, provides an overview of how discourses have shaped the representation of Indonesian women by revealing images that appear explicitly and implicitly in travel stories. Using the Foucauldian discourse analysis approach allowed the writers to exclude subjects, objects, and meanings to discover a comprehensive web of image construction. This research found that Indonesian women’s representation is stuck in an ambiguous position as it strives to be an independent individual subject. Penelitian ini bertujuan untuk menjelaskan representasi perempuan Indonesia melalui cerita perjalanan kontemporer. Pada dasarnya, globalisasi telah menantang kuasa hegemoni wacana yang cenderung menempatkan perempuan Indonesia pada posisi kedua. Akan tetapi, hal tersebut tidak menjamin kesetaraan antara hubungan gender, melainkan terkadang menjadikannya semakin kabur sebagai konsekuensi adanya pertentangan antar wacana. Kajian ini memberikan gambaran bagaimana wacana membentuk representasi perempuan Indonesia dengan menunjukkan citra-citra yang hadir secara eksplisit maupun implisit dalam cerita perjalanan. Dengan menggunakan pendekatan analisis Foucauldian memberikan sebuah kemungkinan kepada kita untuk memisahkan antara subjek, objek dan makna dan untuk menemukan jaring-jaring konstruksi citra yang komprehensif. Penelitian ini menemukan bahwa representasi perempuan Indonesia terjebak dalam posisi yang ambigu yang tampaknya mencoba untuk hadir sebagai subjek independen.
Relación entre el nivel de ansiedad y rendimiento académico en estudiantes de enfermería
Authors: Mosqueira-Soto C. , Poblete-Troncoso M. 
Published: August 2021

Introducción: La ansiedad ante exámenes es un tipo de ansiedad de ejecución y puede afectar el rendimiento académico, en especial en los estudiantes de enfermería que sufren altos niveles de estrés.
Objetivo: Establecer la relación entre el nivel de ansiedad, el rendimiento académico y variables sociodemográficas en estudiantes de enfermería de la Universidad Católica del Maule.
Metodología: Estudio cuantitativo, descriptivo y de corte transversal. La muestra fue censal, de 94 estudiantes. Para la recolección de los datos se utilizaron dos instrumentos: de antecedentes sociodemográficos (edad, género, colegio de procedencia, lugar de origen y ocupación del estudiante); y el Cuestionario de Ansiedad frente a los Exámenes (CAEX). El rendimiento académico se obtuvo del Examen Clínico Objetivo Estructurado (OSCE por sus siglas en inglés). Para el análisis de los datos se usó T-Student y Anova.
Resultados: Se observó una relación significativa entre el rendimiento académico y el semestre que cursaban los estudiantes. Los estudiantes de los semestres más avanzados tuvieron mejor rendimiento (p= 0.015). El alumno que trabaja y estudia presentó mejor rendimiento en el examen OSCE con respecto al que sólo estudia (p= 0.027). El sexo femenino presentó diferencias significativas con el nivel ansiedad (p= 0.038).
Conclusión: Los niveles de ansiedad se encontraron entre los rangos bajos y medios, se sugiere mantenerlos para promover aprendizajes significativos. Resulta necesario analizar la planificación curricular para reestructurar las mallas curriculares y así apoyar a los estudiantes trabajadores al hacer compatible el trabajo con el estudio, con la finalidad de mejorar su calidad de vida.
Eastern red-backed salamanders: A comprehensive review of an undervalued model in evolution, ecology, & behavior
Authors: Fisher-Reid M. Caitlin , Grayson Kristine , Grouleff Sara R. , Hair Madelyn A. , Matlaga Tanya J. Hawley , Ireland Angelina K. , Mead Louise S. , John Alyssa St , Starr Meigan , Sterrett Sean C. , Streeter Kody N. 
Published: August 2021

What makes a model organism? Identifying the qualities of a model
organism has been given a great deal of attention in the biomolecular
sciences, but less so in the fields of evolution, ecology, and behavior
(EEB). In EEB, biotic and abiotic variation are features to understand,
not bugs to get rid of, and EEB scientists often select organisms to
study which best suit the scientific question at hand. Successful EEB
model organisms can be studied at multiple biological scales and have a
wealth of accumulated knowledge on which current research programs
build. A recent call within EEB to invest in the inclusive development
of diverse model systems and scientists has led us to evaluate the
standing of the widespread, abundant, terrestrial salamander we study,
the eastern red-backed salamander (Plethodon cinereus). We first
look at salamanders as EEB models more generally, to determine
where P. cinereus fits in this broader context. We next present a
comprehensive review of the literature on the eastern red-backed
salamander (Plethodon cinereus) since the last comprehensive
review was completed in 1998. The core of our paper reviews 410 recent
studies and highlights inconsistencies, gaps in our knowledge, and
future directions in the context of the 1998 review. Finally, we present
a collaborative research network, SPARCnet, as a nascent infrastructure
for continued research on P. cinereus. Here, we especially
discuss how this type of infrastructure can be broadly applied not just
to other salamanders, but to other model systems, so that the future of
EEB research may benefit from models which accurately represent, in
Darwin’s words, “endless forms most beautiful and most wonderful.”
Respons pertumbuhan pakcoy terhadap asam humat dan Trichoderma dalam media tanam pelepah kelapa sawit
Authors: Rahhutami Ratih , Handini Aline Sisi , Astutik Dwi 
Published: August 2021

AbstrakPemanfaatan limbah organik dari perkebunan sebagai media tanam pakcoy (Brassica chinensis L.) diharapkan dapat ditingkatkan dengan penggunaan pupuk organik serta pupuk hayati. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) pola faktorial. Faktor pertama adalah dosis asam humat meliputi 1, 3, dan 5 g. Faktor kedua adalah dosis Trichoderma sp., meliputi 50, 100, dan 150 mL. Data yang diperoleh kemudian dianalisis ragam pada taraf nyata 5%. apabila terdapat pengaruh nyata, dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa aplikasi asam humat dan Trichoderma sp. memiliki pengaruh mandiri dan tidak terdapat interaksi. Dosis asam humat 3 g per tanaman menghasilkan jumlah daun, panjang daun, tinggi tanaman, tinggi tanaman, bobot basah, dan bobot kering tanaman lebih tinggi dibanding dosis 1 dan 5 g. Perlakuan Trichoderma sp. dosis 50 mL per tanaman memiliki pengaruh lebih baik terhadap jumlah daun, panjang daun, tinggi tanaman, dan bobot basah tanaman.Kata Kunci: hortikultura, jamur, morfologi, senyawa organik Abstract The utilization of organic farm estate as pakcoy (Brassica chinensis L.) growing media may improved by using biofertilizer and organic fertilizer. The research used factorial randomized block design. First factor was humic acid dosage, which included 1, 3, and 5 g of humic acid. Second factor was Trichoderma sp. dosage, which included 50, 100, and 150 mL of Trichoderma sp. Data were analyzed using ANOVA at 5% level, then continued by DMRT test. The results showed that the application of humic acid and Trichoderma sp. had single effects and there was no interaction. The dosage of humic acid 3 g per plant had higher number of leaves, leaf length, plant height, wet weight, and dry weight than other dosages. The treatment of Trichoderma sp. at dosage of 50 mL per plant had a better effect on the number of leaves, leaf length, plant height, and plant wet weight.Keywords: fungi, horticulture, morphology, organic compounds
Dwa znaki (mofet) Ezechiela (Ez 12,1-16; 24,24-27)
Authors: Jasiński OFM Andrzej Sebastian 
Published: July 2021

Ezechiel był kapłanem, ale został powołany na urząd proroka w tym momencie historii Izraela, kiedy niektórzy Izraelici znajdowali się w niewoli. Ezechiel był już w Babilonie, ale głosił do tych, którzy wciąż byli w Jerozolimie; którzy byli przekonani, że ich kłopoty są tylko tymczasowe i nie dostrzegali nieuchronności zbliżającego się sądu Bożego nad nimi. Symboliczne działania proroka były bardzo ważne w jego przesłaniu. Poselstwo Ezechiela: upadek Jerozolimy i niewola były koniecznymi środkami dla Boga, aby wyjaśnić, że teokracja Judy w jej zewnętrznej formie musi dobiec końca z powodu grzechu.Ezechiel w swojej Księdze czterokrotnie używa terminu מוֹפֵת („znak”; Ez 12,6.11; 24,24.27). W Ez 12,4-6 prorok miał zrealizować swoją przypowieść o uciekinierach. Jego pantomima składa się z bezsłownych gestów, mających na celu zwrócenie uwagi i wywołanie pytań. Ezechiel służył jako znak dla domu Izraela, ostrzeżenie przed zbliżającą się zagładą Jerozolimy (Ez 12,6). Dla wygnańców Ezechiel był znakiem, ilustracją lub lekcją poglądową (Ez 12,11). To, co uczynił w symbolicznym działaniu, w rzeczywistości spotka mieszkańców Jerozolimy, którzy zostaną wypędzeni ze swojej ojczyzny (wygnanie) i zmuszeni do osiedlenia się na terenach wydzielonych dla nich przez zdobywców (niewola).Prorokowi nakazano powstrzymanie się od lamentacji i żałoby po śmierci żony (Ez 24,15-27). Był to kolejny znak Ezechiela dla wygnańców (Ez 24,24). Gdy po wszystkich wydarzeniach nadejdzie wiadomość, że Jerozolima upadła, a świątynia została zburzona, będą wiedzieli, że wydarzenie to zostało zadekretowane przez Pana. Znak śmierci jego żony zapowiada sąd i śmierć; ten znak będzie szerzył wieść o zbawieniu i życiu (Ez 24,27).
Partially thrombosed middle cerebral artery-lenticulostriate artery aneurysm with native radiological examinations suggesting proximal lenticulostriate artery aneurysm: A case report
Authors: Anan Mitsuhiro , Nagai Yasuyuki , Matsuda Takeshi , Morimoto Kazuya , Fujiki Minoru 
Published: August 2021

Background:
Preservation of the lenticulostriate artery (LSA) is crucial. LSAs usually cannot be spared with LSA aneurysms, when surgical clipping/excision or endovascular embolization of the LSA itself is performed. On the other hand, the LSA should be separated and preserved for proximal middle cerebral artery (M1)-LSA aneurysms.

Case Description:
We report a case of M1-LSA aneurysm with native radiological examinations suggesting LSA aneurysm. The highlight of this unusual case was that during surgery, the aneurysm orifice was almost covered with thrombus and blood flow in an aneurysm that appeared separate from M1. Partial thrombectomy-clip reconstruction was performed, and M1 and LSAs were well preserved.

Conclusion:
Even with currently developed radiological modalities, thrombosed intracranial aneurysms may be misdiagnosed, depending on intraluminal flow conditions. Intraoperative findings from craniotomy sometimes contribute to a better understanding of the pathophysiology and decisions on appropriate treatment strategy.