bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of videos with AI-generated images to provide students with authentic and diverse insights into STEM occupations
Authors: Romanik Max , Wegner Claas 
Published: February 2025

Societies around the world are facing a shortage of STEM professionals. In this context, the lack of students' interest in STEM-related fields of study and occupations, especially of underrepresented groups, needs to be addressed. This article reports on the results of an extracurricular STEM intervention with elements of image-generating artificial intelligence (AI) as a career orientation measure. The AI-generated images were edited into videos with auditory explanations, shedding light on various aspects of the work of STEM professionals and aiming to show a diverse picture of people working in STEM occupations. A quantitative pre-post survey with a control group (N=137) showed that the videos had no significant influence on STEM interest (p = .374), career-related STEM self-efficacy expectations (p = .422), career-related STEM outcome expectations (p = .896) and STEM career aspirations (p = .780). At the same time, there were indications that the videos had a non-significant beneficial effect. A more prominent integration of the videos into the lesson context and in a more diverse sample may lead to positive effects of video use in the future.
Perancangan Sistem Pendingin pada Prototipe Mesin Injeksi Molding Double Barrel
Authors: Maulana Fikri , Mulyono Sugeng , Muslimin Muslimin 
Published: February 2025

Kebutuhan produk plastik semakin meningkat di Indonesia, menurut Datareksa Institute pada tahun 2023, meningkat sebesar 5% dalam lima tahun terakhir dan diproyeksikan terus meningkat sebesar 6% dalam 10 tahun ke depan. Observasi pada prototipe mesin mesin injeksi molding double barrel di laboratorium pengembangan produk teknik mesin Politeknik Negeri Jakarta menunjukkan beberapa kekurangan seperti sistem pendingin tidak efektif yang berdampak pada kualitas produk dan siklus produksi yang lama. Memodifikasi prototipe mesin injecksi molding double barrel dengan mengintegrasikan sistem pendingin air pada cetakan molding dapat mengatasi kekurangan prototipe sebelumnya dengan menambahkan sistem pendingin air yang terhubung langsung pada cetakan molding. Metode penelitian yang digunakan adalah metode VDI 2221 yang melibatkan mendesain sistem pendingin, pembuatan dan pengujian prototipe dengan simulasi Dinamika fluida komputasi atau CFD serta evaluasi kerja pada sistem pendingin untuk meningkatkan kualitas produk dan efisiensi siklus produksi. Hasil penelitian menunjukkan bahwa durasi pendinginan sebesar 32 detik dengan suhu akhir 41 derajat celsius artinya penambahan sistem pendingin air mampu mengurangi durasi siklus produksi.
¿Cuál es el problema? El aborto y la importancia de una maternidad deseada en Colombia
Authors: Dimas Hoyos Johana Valentina , Sanabria Navarro Daniela , Velásquez Rojas Lucas David , Niño Marín Juan Sebastián 
Published: February 2025

Desde que se ha vivido en sociedad, siempre se ha tenido un rol en la mujer en donde no se le ha permitido tomar decisiones por su cuenta. Es por ello que hemos arrastrado hasta nuestros días, en cuestiones como el aborto donde muchas personas se ven inmiscuidas dando mucho de qué hablar y queremos develar cuáles son sus motivos. Dentro de lo que corresponde a este tema, existen múltiples referentes que han dado bases de la situación en específico de Colombia. Uno de los principales siendo Corpas (2016) en donde se plantean las bases tanto sociales como de carácter jurídico. Lo cual se desarrolla en campos como las características de la sociedad colombiana. A partir de lo anterior se buscó caracterizar la problemática con un componente humano a través de la realización de una encuesta de percepción a 21 personas en donde se pudo comprobar que hay una polarización clara en lo que respecta al aborto. Sin embargo, hemos podido encontrar que hay un componente muy importante que tanto investigadores como encuestados hemos podido concordar, y es que una educación sexual de calidad y bien implementada puede ayudar de raíz a esta problemática
Propuestas para reorientar la actividad económica del municipio minero de Sutatausa por medio del emprendimiento
Authors: Mendoza Moreno Saúl , Hernández Ballesteros Maria Fernanda , Rubio Santana Mariluz , Díaz Osorio Leyla Adriana 
Published: February 2025

El Municipio de Sutatausa ha basado su economía en la explotación del carbón la cual representa más del 80% de sus ingresos; como consecuencia se presentan afectaciones en los ecosistemas, deterioro del suelo, contaminación del agua y el aire, y a futuro el agotamiento de las reservas mineras con impactos económicos. Esto exige el planteamiento de nuevos espacios productivos, y nuevos escenarios para la generación de emprendimientos de la población. La pregunta problema planteada es: ¿cómo reorientar la actividad económica del municipio minero de Sutatausa por medio del emprendimiento? La metodología será mixta, el diseño será la investigación acción participativa (IAP). Como resultado se identifican las líneas de emprendimiento a desarrollar, las necesidades en materia de formación y desarrollo de capacidades para emprender y la creación de una Unidad para el desarrollo de emprendimientos que acompañe a la comunidad para orientar sus emprendimientos y encaminarlos hacia la sostenibilidad.
EndoGene database: reported genetic variants for 5,926 Russian patients diagnosed with endocrine disorders
Authors: Buzdin Anton A. , Zolotovskaia Marianna A. , Roumiantsev Sergey A. , Emelyanova Aleksandra G. , Golounina Olga O. , Pugacheva Polina A. , Luppov Daniil V. , Kuzminyh Anastasia V. , Alexeeva Arseniya O. , Emelianova Anna A. , Novoselov Alexey L. , Matrosova Alina , Slepukhina Anastasia A. , Popov Sergey V. , Plaksina Evgeniya V. , Petrov Vasiliy M. , Guselnikova Anastasia R. , Shagina Anastasia D. , Suntsova Maria V. , Zakharova Victoriya V. , Belaya Zhanna E. , Vorontsova Maria V. , Melnichenko Galina A. , Mokrysheva Natalia G. , Chekhonin Vladimir P. , Dedov Ivan I. 
Published: February 2025

IntroductionEndocrine system disorders are a serious public health burden and can be caused by deleterious genetic variants in single genes or by the combined effects of multiple variants along with environmental and lifestyle factors.MethodsThe EndoGene database presents the results of next-generation sequencing assays used to genetically profile 5,926 patients who were diagnosed with 450 endocrine and concomitant diseases and were examined and treated at the National Medical Research Center for Endocrinology between November 2017 and January 2024. Among them, 494, 1,785, 692, and 1,941 patients were profiled using four internally developed genetic panels including 220, 250, 376, and 382 genes, respectively, selected based on a literature analysis and clinical recommendations, and 1,245 patients were profiled by whole exome sequencing covering 31,969 genes.Results2,711 genetic variants were reported as clinically relevant by medical geneticists and are presented here along with genomic, technical, and clinical annotations.DiscussionThis publicly accessible database will be useful to those interested in genetics, epidemiology, population statistics, and a better understanding of the molecular basis of endocrine disorders.
Metformin-mediated intestinal AMPK activation ameliorates PCOS through gut microbiota modulation and metabolic pathways
Authors: Xu Yating , Ning Li , Si Yu , Li Xiu , Wang Ruyue , Ren Qingling 
Published: February 2025

IntroductionPolycystic ovary syndrome (PCOS) is a complex disorder characterized by metabolic and ovulatory dysfunctions, often associated with an imbalance in gut microbiota. Despite current treatments, effective management strategies targeting underlying mechanisms remain limited.MethodsIn this study, we used a rat model of PCOS induced by letrozole and a high-fat diet. The effect of intestinal AMP-activated protein kinase (AMPK) activation was evaluated through metformin administration, the most commonly used AMPK activator. We analyzed metabolic parameters, ovulatory functions, gut microbiota composition, and serum levels of Indole-3-carboxaldehyde (I3A), a metabolite involved in inflammation and apoptosis regulation.ResultsMetformin treatment significantly reversed metabolic disorders and restored ovulatory functions in PCOS rats. Moreover, metformin treatment led to notable improvements in gut microbiota composition and an increase in serum I3A levels, which have been shown to mitigate inflammation and apoptosis.DiscussionThis study highlights the therapeutic potential of targeting intestinal AMPK in managing PCOS. By improving both metabolic and reproductive health, activation of AMPK may offer a promising approach for restoring physiological balance in PCOS patients.
Dynamics of spike-specific neutralizing antibodies across five-year emerging SARS-CoV-2 variants of concern reveal conserved epitopes that protect against severe COVID-19
Authors: Zayou Latifa , Prakash Swayam , Vahed Hawa , Dhanushkodi Nisha Rajeswari , Quadiri Afshana , Belmouden Ahmed , Lemkhente Zohra , Chentoufi Aziz , Gil Daniel , Ulmer Jeffrey B. , BenMohamed Lbachir 
Published: February 2025

IntroductionSince early 2020, several SARS-CoV-2 variants of concern (VOCs) continue to emerge, evading waning antibody mediated immunity produced by the current Spike-alone based COVID-19 vaccines. This caused a prolonged and persistent COVID-19 pandemic that is going to enter its fifth year. Thus, the need remains for innovative next generation vaccines that would incorporate protective Spike-derived B-cell epitopes that resist immune evasion.MethodsTowards that goal, in this study we (i) Screened the sequences of Spike among many VOCs and identified conserved and non-conserved linear B-cell epitopes; (ii) Compared titers and neutralization antibodies specific to these conserved and non-conserved B-cell epitopes from serum of symptomatic and asymptomatic COVID-19 patients that were exposed to multiple VOCs across the 5-year COVID-19 pandemic, and (iii) Compared protective efficacy of conserved versus non-conserved B-cell epitopes against the most pathogenic Delta variant in a “humanized” ACE-2/HLA transgenic mouse model.ResultsWe found robust conserved B-cell epitope-specific antibody titers and neutralization in sera from asymptomatic COVID-19 patients. In contrast, sera from symptomatic patients contained weaker antibody responses specific to conserved B-cell epitopes. A multi-epitope COVID-19 vaccine that incorporated the conserved B-cell epitopes, but not the non-conserved B-cell epitopes, significantly protected the ACE2/HLA transgenic mice against infection and COVID-19 like symptoms caused by the Delta variant.DiscussionThese findings underscore the importance of conserved B-cell epitopes in generating robust protective immunity against severe COVID-19 symptoms caused by various VOCs, providing valuable insights for the development of broad-spectrum next generation Coronavirus vaccines capable of conferring cross-variant protective immunity.
Transcriptomics and microbiome insights reveal the protective mechanism of mulberry-derived postbiotics against inflammation in LPS-induced mice
Authors: Abbas Zaheer , Tong Yucui , Zhang Jing , Sammad Abdul , Wang Junyong , Ahmad Baseer , Wei Xubiao , Si Dayong , Zhang Rijun 
Published: February 2025

BackgroundNatural food-derived bioactive compounds have garnered increasing attention for their potential to modulate immune responses and promote gut health. In particular, compounds like mulberry-derived postbiotics (MDP) may offer novel therapeutic strategies to address inflammation, a key driver of many metabolic disorders.MethodologyThis study examines the protective effects of MDP against inflammation in LPS-induced mice, using transcriptomic and microbiome analyses to explore underlying mechanisms.ResultsMDP pretreatment alleviates LPSinduced villous atrophy and intestinal barrier damage, promoting recovery of intestinal morphology. Transcriptomic profiling revealed significant changes in gene expression, with 983 upregulated and 1220 downregulated genes in the NC vs LPS comparison, and 380 upregulated and 204 downregulated genes in the LPS vs LPS+MDP comparison. Enrichment analysis using GO and KEGG pathways revealed significant associations with transcriptional regulatory activity, and the NOD-like receptor signaling pathway among the differentially expressed genes. Protein-protein interaction analysis identified key genes involved in inflammation and immune regulation, with hub genes like IL6, CXCL10, and MYD88 in the LPS group and CD74, CIITA, and H2-AB1 in the MDP-treated group.ConclusionMicrobiome analysis suggested MDP may also influence gut microbiota composition, supporting systemic immune regulation. These findings highlight MDP’s potential as a food additive for immune modulation and gut health.
CAMTA1-immunonegative epithelioid hemangioendotheliomas of the liver: a clinicopathological and molecular analysis of seven cases
Authors: Nie Yang , Jing Wenyi , Zheng Xuanxuan , He Xin , Chen Min , Zhang Hongying 
Published: February 2025

BackgroundEpithelioid hemangioendothelioma (EHE) is a rare malignant vascular tumor. Most EHEs (&gt;90%) cases harbor WWTR1::CAMTA1 fusion gene, and CAMTA1 immunohistochemistry (IHC) is a highly sensitive and specific tool for EHE diagnosis. However, there exist CAMTA1-immunonegative cases, the majority of which harbor YAP1::TFE3 fusion, with a few cases having more rare fusions. Liver is one of the most common sites of EHE, where the CAMTA1 subtype dominates, and the other variants are extremely rare. Hence, we focused on the hepatic CAMTA1-immunonegative EHEs to analyze the clinicopathological and molecular features of these peculiar cases.MethodsThe SNOMED search of the hospital pathology files between January 2016 to November 2023 identified 57 hepatic EHEs and 7 cases were CAMTA1-immunonegative. Fluorescence in situ hybridization (FISH), next generation sequencing (NGS) and Sanger sequencing were performed to identify the genetic change of the 7 cases.ResultsThis series included 3 females and 4 males, aged from 33 to 64 years. All the 7 cases were negative for CAMTA1 IHC. Four cases were positive for TFE3 IHC and exhibited YAP1::TFE3 fusion. Another 3 cases were also negative for TFE3, while WWTR1::CAMTA1 fusion were detected by NGS in 1 case and demonstrated by FISH in all the 3 cases. Morphologically, among the 4 TFE3 rearrangement cases, 3 cases showed the TFE3-sutype morphologic appearance, while the histology of 1 case was similar to that of CAMTA1- subtype. In the 3 CAMTA1-rearranged lesions, 2 cases had classic EHE morphology, and 1 case exhibited atypical histology, with higher atypia and well-formed vessels. Surgical resection was performed on five cases and two cases were biopsied and received chemotherapy. Follow-up information was available in 6 patients (median 46 months), including 4 patients were alive without disease and 2 patients were alive with disease.ConclusionOur study reported 7 CAMTA1-immunonegative hepatic EHEs and most of them were TFE3-rearranged EHEs with morphology variation. Moreover, there does exist the CAMTA1-immunonegative but CAMTA1-rearranged EHE cases. Therefore, the diagnosis of EHE should be based on morphology, combined with CAMTA1 and TFE3 IHC, and if necessary, supplemented by genetic analysis including FISH and NGS, to establish correct diagnosis.
Diagnostic utility of MRI-based convolutional neural networks in soft tissue sarcomas: a mini-review
Authors: Voigtländer Hendrik , Kauczor Hans-Ulrich , Sedaghat Sam 
Published: February 2025

PurposeThis review assesses the diagnostic performance of MRI-based convolutional neural networks for identifying and grading soft tissue sarcomas, evaluating therapy responses, and assessing the risk for metastases and recurrences.MethodsElectronic databases, specifically PubMed/MEDLINE and Google Scholar, were diligently scoured for studies that delved into the intersection of convolutional neural networks, soft tissue sarcomas, and MRI. Three topics were included: 1) differentiating and grading soft tissue sarcomas, 2) assessing therapy response, and 3) predicting metastases and recurrences.ResultsThis review included 12 articles. Seven articles investigated the differentiation and grading of soft tissue sarcomas. Sensitivity for that issue ranged from 0.85 to 0.95, specificity from 0,33 to 1, and the area under the curve (AUC) from 0.74 to 0.96. Three articles investigated therapy responses, and two discussed metastasis and recurrence prediction. Only one article out of the five articles above presented accurate diagnostic values. That article examined the prediction of lung metastases and demonstrated a sensitivity of 0.47, a specificity of 0.97, and an AUC of 0.83.ConclusionAI applications using CNNs demonstrated robust capabilities in differentiating and grading soft tissue sarcomas using MRI. However, studies on therapy response and prediction of metastases and recurrences are still lacking.
The indirect effect of sleep quality on stress-related psychosocial outcomes in adolescents: an investigation across genders
Authors: Koike Camila , Nestor Bridget A. , Baumer Andreas M. , Kossowsky Joe 
Published: February 2025

BackgroundSleep is foundational for adolescent psychosocial outcomes though often compromised by normative developmental changes and external factors.MethodsThis cross-sectional study examined sleep quality as a mechanism linking stress and psychosocial outcomes and explored gender differences.ResultsAdolescents (N = 236; Mean = 15.66; SD = 1.07, 46.19% female) completed self-report measures assessing sleep quality and psychosocial outcomes. Structural equation modeling was used to test the potential mediating effect of sleep quality on associations between stress and psychosocial outcomes. Results indicated sleep quality accounted for 82.0% of the total effect of stress on school functioning (β = −0.45, p &lt; 0.001) and 61.9% of the total effect of stress on pain (β = 0.14, p &lt; 0.001). A larger indirect effect of sleep quality on school functioning (βdiff = −0.27, p = 0.006) emerged for boys than girls, and the effect of sleep quality on pain was significant only for girls (β = 0.20, p = 0.003, 65.0% of total effect).ConclusionSleep quality explained a large proportion of the cross-sectional association between stress and school functioning and pain. Sleep quality represents a modifiable transdiagnostic pathway that may buffer the effects of stress in adolescence.
Restricted economic activity due to health conditions and risk of depression: results from the Korean Longitudinal Study of Aging
Authors: Yun Il , Kim Jae-Hyun , Moon Jong Youn 
Published: February 2025

PurposeThis study aimed to investigate the association between the restricted economic activity due to health conditions and risk of depression, and further evaluate the differences in this association according to gender and across the older people.MethodsData from the KLoSA from 2006 to 2016 was used and 10,144 research samples were included at baseline at 2006. Generalized estimating equation (GEE) model was applied in this longitudinal analysis.ResultsOf the 10,144 individuals at baseline 2006, the odds ratio (OR) of depressive symptoms in those responding “very probable” on restricted economic activity was 2.88 times higher (p-value: &lt;0.0001) compared with “not at all” respondents of restricted economic activity. In 64 years or less and 65 years or more, OR of depressive symptoms in “very probable” respondents of restricted economic activity was 3.03 times higher (p-value: &lt;0.0001) and 2.85 times higher (p-value: &lt;0.0001) compared with “not at all” respondents of restricted economic activity, respectively. In male and female, OR of depressive symptoms in “very probable” respondents of restricted economic activity was 1.76 times higher (p-value: &lt;0.0001) and 1.56 times higher (p-value: &lt;0.0001) compared with “not at all” respondents of restricted economic activity, respectively.ConclusionThis study demonstrated that restricted economic activity due to adverse health conditions was associated with risk of depression, especially among men between the ages of 45 ~ 64. These results suggest that in order to improve mental health in the middle-aged people, public health interventions are needed to sustain economic activity.
Prevalence and associated factors with oral frailty in middle-aged and older hospitalized patients: a cross-sectional study
Authors: Dou Jun-kai , Liu Huan , Min Jiahui , Luo Yang , Liu Qian , Shi Xue-zhi , Tao Xiubin , Zhang Ming 
Published: February 2025

BackgroundWith the development of the economy and society, people pay more and more attention to oral health. Oral frailty can limit nutritional intake and make an individual physically weak, which is detrimental to people’s health. Therefore, it is urgent to identify oral frailty and their associated risk factors. In this study, we aimed to evaluate the oral frailty and its influencing factors in Chinese middle-aged and older hospitalized patients.MethodsA cross-sectional study was conducted from May 2023 to February 2024 in 2 tertiary hospitals in Wuhu City, Anhui Province, China. The self-designed sociodemographic information, the Oral Frailty Index-8, the 2-item Connor–Davidson resilience scale (CD-RISC-2), the sarcopenia screening questionnaire, and the three-item short literacy survey were used in this study.ResultsA total of 914 middle-aged and older adults patients were recruited. The prevalence of oral frailty was 48.7% (445/914). In the univariate analysis, oral frailty was significantly associated with age, education, place of residence, monthly income, sarcopenia, resilience, and health literacy. Correlation analysis showed that oral frailty was significantly negatively correlated with sarcopenia, resilience, and health literacy. In the binary logistic regression analysis, oral frailty was significantly associated with sarcopenia, and health literacy. Health literacy was a protective factor of oral frailty.ConclusionThis study aimed to investigate the prevalence of oral frailty and identify the associated influencing factors among middle-aged and older adults patients in Anhui Province, China. This study identified several factors influencing oral frailty in middle-aged and older adults patients. Therefore, the government and relevant departments should implement targeted interventions to improve middle-aged and older adults patients’ oral frailty.