bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vacuolar localization via the N-terminal domain of Sch9 is required for TORC1-dependent phosphorylation and downstream signal transduction
Authors: Novarina Daniele , Guerra Paolo , Milias-Argeitis Andreas 
Published: July 2021

AbstractThe budding yeast Sch9 kinase (functional ortholog of the mammalian S6 kinase) is a major effector of the Target of Rapamycin Complex 1 (TORC1) complex in the regulation of cell growth in response to nutrient availability and stress. In budding yeast, Sch9 is partially localized at the vacuolar surface, where it is phosphorylated by TORC1 under favorable growth conditions. Sch9 recruitment at the vacuole is mediated by direct interaction between PI(3,5)P2 on the vacuolar membrane and the region of Sch9 encompassing amino acid residues 1-390, which contains a C2 domain. Since many C2 domains mediate phospholipid binding, it had been suggested that the C2 domain of Sch9 mediates its vacuolar recruitment. However, the in vivo requirement of the C2 domain for Sch9 localization had not been demonstrated, and the phenotypic consequences of Sch9 delocalization remained unknown. Here, by examining cellular localization, phosphorylation state and growth phenotypes of Sch9 truncation mutants, we show that deletion of the N-terminal domain of Sch9 (aa 1-182), but not the C2 domain (aa 183-399), impairs vacuolar localization and TORC1-dependent phosphorylation of Sch9, while causing growth defects similar those observed in sch9Δ cells. Artificial tethering of an N-terminally truncated Sch9 mutant at the vacuolar membrane rescued TORC1-dependent phosphorylation and cell growth. Our study uncovers a key role for the N-terminal domain of Sch9 and demonstrates that recruitment of Sch9 at the vacuolar surface is necessary for TORC1-dependent phosphorylation and downstream signal transduction for the regulation of cell growth.
Cytoplasmic organization promotes protein diffusion
Authors: Huang William Y. C. , Cheng Xianrui , Ferrell James E. 
Published: July 2021

ABSTRACTThe cytoplasm is highly organized. However, the extent to which this organization influences the dynamics of cytoplasmic proteins is not well understood. Here, we used Xenopus laevis egg extracts as a model system to study diffusion dynamics in organized versus disorganized cytoplasm. Such extracts are initially homogenized and disorganized, and will self-organize into cell-like units over the course of 20-60 min. Using fluorescence correlation spectroscopy, we observed that self-organization is accompanied by changes in protein diffusivity; as the extract organizes, proteins diffuse about twice as quickly over a length scale of a few hundred nanometers. Even though the ordered cytoplasm contained organelles and cytoskeletal elements that might be expected to interfere with diffusion, after self-organization took place, the speed of protein diffusion approached that of organelle-depleted cytosolic extracts. This finding suggests that subcellular organization optimizes protein diffusivity. The effect of organization on diffusion varies with molecular size, with the effects being largest for protein-sized molecules. These results show that cytoplasmic organization promotes the efficient diffusion of protein molecules in a densely packed environment.
Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia
Authors: Gong Rui , Jiang Fangfang , Moreland Zane G. , Reynolds Matthew J. , Espinosa de los Reyes Santiago , Gurel Pinar S. , Shams Arik , Bowl Michael R. , Bird Jonathan E. , Alushin Gregory M. 
Published: July 2021

SummaryThe motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and myosin-15 mutations cause profound deafness. In a companion study, we report that myosin-15 nucleates actin filament (“F-actin”) assembly and identify a progressive hearing loss mutation (p.D1647G, “jordan”) which disrupts stereocilia elongation by inhibiting actin polymerization. Here, we present cryo-EM structures of myosin-15 bound to F-actin, providing a framework for interpreting deafness mutations and their impacts on myosin-stimulated actin assembly. Rigor myosin-15 evokes conformational changes in F-actin yet maintains flexibility in actin’s D-loop, which mediates inter-subunit contacts, while the jordan mutant locks the D-loop in a single conformation. ADP-bound myosin-15 also locks the D-loop, which correspondingly blunts actin-polymerization stimulation. We propose myosin-15 enhances polymerization by bridging actin protomers, regulating nucleation efficiency by modulating actin’s structural plasticity in a myosin nucleotide-state dependent manner. This tunable regulation of actin polymerization could be harnessed to precisely control stereocilium height.
Barriers to adoption of reverse logistics: a case of construction, real estate, infrastructure and project (CRIP) sectors
Authors: Ambekar Sudhir , Roy Dipayan , Hiray Amit , Prakash Anand , Patyal Vishal Singh 
Published: July 2021

PurposeThis study attempts to identify and analyse the barriers to implementing a reverse logistics (RL) system in Indian Construction, Real estate, Infrastructure and Project (CRIP) sectors and present a structured model to identify interdependencies among them.Design/methodology/approachThe barriers to implementing RL in CRIP sectors in India were identified using a Delphi study. The interdependencies were identified using Interpretive Structural Modeling (ISM). Further, using the Matriced' Impacts Croisés Multiplication Appliquée à un Classement (MICMAC) analysis, the barriers were classified on the basis of their driving power and interdependencies.FindingsThe study has identified ten barriers that can hamper the application of an RL system in CRIP sectors. The finding of the ISM model shows that macro level barriers such as lack of awareness of reverse logistics, insufficient government policies and unavailability of standard codes stimulate each other and also drive all other barriers. The organization-specific barriers operating at the strategic/tactical level, namely, company's rigid mechanism, lack of awareness of economic profits, inadequate company’s organizational policies and lack of training, reluctance from stakeholders, scarcity of resources and finance from company are found at the intermediate level of hierarchy and they can be influenced by the barriers at the lower level and influence the barriers on the and higher levels. The operational level barrier namely “Inadequate Information Technology system” is at the top of the hierarchy and can be driven by all the barriers at the lower level.Research limitations/implicationsThe present findings are based on the opinions of experts only from Indian CRIP sectors so the results may require to be validated in other contexts.Practical implicationsThe structural model presenting the interdependencies will be a guide for the CRIP supply chain professionals in understanding and ranking the barriers they may face while implementing the RL system.Originality/valueThe study contributes to the existing literature by providing a set of barriers and their interdependencies faced during the implementation of an RL system implementation in CRIP sectors. It is one of the first studies which identifies barriers applicable to the CRIP firms in India and models their inter-dependencies. Additionally Consequently, these firms can make a move forward towards a circular economy by overcoming these interlinked barriers.
Exploring the impact of abusive supervision on employee' voice behavior in Chinese construction industry: a moderated mediation analysis
Authors: Khan Naseer Abbas , Khan Ali Nawaz 
Published: July 2021

PurposeThe purpose of this study is to explore the impact of abusive supervision on employees' voice in China's construction industry. Moreover, the authors explore the mediating role of ethics-related self-efficacy and work engagement and the moderating influence of psychological climate in explaining the association between abusive supervision and employee voice behavior.Design/methodology/approachThis study used data in pairs collected from 402 supervisors and employees of construction companies in Anhui, China. In this study, the authors used the time-lag approach to collect data in three-time waves from different respondents. A structural equation modeling (SEM) approach was applied to test the hypothesized model.FindingsThe results of this study indicate that there is a significant association between abusive supervision and employee voice. Moreover, the results indicated that work engagement mediated the association between abusive supervision and employees' voice. In contrast, self-efficacy did not mediate the link between abusive supervision and employee voice. Furthermore, results also show that the contingent effect of psychological climate significantly influences the mediating effect of work engagement.Originality/valueThis study also has implications for the construction industry, allowing managers to create a favorable working atmosphere in which employees can reinforce their voices at work.
Understanding whole school physical activity transition from a practice theory perspective
Authors: Spotswood Fiona , Vihalemm Triin , Uibu Marko , Korp Leene 
Published: July 2021

PurposeIn this study, the authors offer a practice theory framing of school physical activity transition with conceptual and managerial contributions to whole school approaches (WSAs).Design/methodology/approachBased on a literature overview of the limitations of WSA, ecological and systems theorisation and a practice theory framing of physical activity, the authors introduce a model that identifies signs of practice transition and conceptualises the relationship between signs and practice reconfigurations. To exemplify insights from the model, the authors provide illustrations from three cases from the national Estonian “Schools in Motion” programme.FindingsThe signs of practitioner effort, resistance and habituation indicate how practice ecosystem transition is unfolding across a spectrum from practice differentiation to routinisation. Several signs of transition, like resistance, indicate that reconfigured practices are becoming established. Also, there are signs of habituation that seemingly undermine the value of the programme but should instead be celebrated as valuable evidence for the normalisation of new practices.Practical implicationsThe article provides a model for WSA programme managers to recognise signs of transition and plan appropriate managerial activities.Originality/valueThe practice theory framing of school physical activity transition advances from extant theorizations of WSAs that have failed to account for the dynamic ways that socio-cultural change in complex school settings can unfold. A model, based on a practice ontology and concepts from theories of practice, is proposed. This recognises signs of transition and can help with the dynamic and reflexive management of transition that retains the purpose of systemic whole school change.
The impact of social media marketing activities on brand equity in the banking sector in Bangladesh: the mediating role of brand love and brand trust
Authors: Hafez Md. 
Published: July 2021

PurposeThis research aims to explore the impact of social media marketing (SMM) activities on brand equity (BE) in the banking sector in Bangladesh. Moreover, brand love and brand trust are examined as a mediator of SMM activities and BE.Design/methodology/approachData were collected from a total of 289 banking customers in Bangladesh through a structured questionnaire and the hypotheses were examined using structural equation modeling (SEM).FindingsThe results validated that SMM activities have no significant influence on BE directly. Furthermore, brand love fully mediates the linkage between SMM activities and BE. Likewise, brand trust was found to have a partial mediation effect on SMM activities and BE.Research limitations/implicationsThis study was based on a specific sector in a particular geographic area. Hence, cross-cultural studies on different sectors need to be conducted to generalize the findings of the current research.Practical implicationsThe study offers useful insights for bank marketers to successfully manage SMM activities that can generate consumer interest toward a bank's brand and prevent switching behavior. Furthermore, the proliferation of authentic brand-related information over a firms' social media pages can build strong brand trust, which in turn contributes to BE for the banks.Originality/valueThe study further extended the current knowledge by showing that how SMM activities influence BE in the banking sector in Bangladesh. Also, this study empirically corroborates the mediation influence of brand love and brand trust on SMM activities and BE in the banking sector in Bangladesh, which was rarely tested in prior studies. Hence, the findings will add value to the nascent literature of BE from an SMM perspective.
Preferences for crop insurance attributes among cocoa farmers in Ghana
Authors: Owusu Victor , Abdulai Awudu , Ali Williams 
Published: July 2021

PurposeThis article analyzes farmers' preferences for different nonindexed crop insurance alternatives, using discrete choice experiment data on cocoa farmers from southern Ghana. We examine farmers' attendance to attributes by comparing self-reported attribute nonattendance (ANA) to the behavior inferred from the choices.Design/methodology/approachWe utilize the latent class endogenous attribute attendance (EAA) model to address potential endogeneity by jointly modelling farmers' attribute processing strategies with their choice of attributes of the insurance products.FindingsThe results show that premium levels, mode and length of indemnity payouts tend to influence farmers' preferences for crop insurance products. The findings also reveal that credit-constrained farmers attend more to premium and payment mode attributes of the crop insurance products and that credit-constrained farmers tend to exhibit lower willingness-to-pay estimates for the crop insurance attributes.Research limitations/implicationsThe findings from the study suggest that credit constraints do not only limit input use, but also tend to have statistically significant impact on farmers' cocoa insurance participation decisions.Originality/valueThe study examines the impact of credit constraints on farmers' crop insurance preferences while accounting for ANA.
A holistic study of factors governing small and medium enterprises in India
Authors: Bagodi Virupaxi , Raravi Prasannna P. 
Published: July 2021

PurposeThe purpose of this paper is to identify the input, process and output factors (along with their manifest variables) of small and medium enterprises (SMEs), and to establish cause and effect relationships amongst the factors and sub-factors. Systems thinking, a holistic approach, is used to carry out qualitative analysis of the feedback loops.Design/methodology/approachA well-structured questionnaire was developed to gather the relevant data to identify the factors affecting the performance of SMEs in a holistic manner. A total of 150 responses were collected during November 2015–March 2016. Factor analysis and path analysis were used to establish causal relationships between input, process and output factors. The systems thinking approach has been used for qualitative analysis.FindingsFeedback loops have been identified amongst input-process-output-input factors and amongst sub-factors. They enabled authors to infer that the managers/owners of SMEs are systems thinkers, if not completely, at least partially. Six negative feedback loops and one positive feedback loop prevail. System behaviour arises out of the interaction of positive and negative feedback loops; it appears that in the long-run, the SMEs attain their target levels. The following inferences are drawn: circular relationships are identified amongst input, processes and organisational performance (OP), modern management tools such as just in times, Kanban have long-term benefits and are perceived as ineffective by small enterprises and formal financing and functional transparency enhances OP.Originality/valueSystems thinking, a holistic approach, has been used to study the effect of input, process and output factors on one another. Such studies are sparse, especially, in the Indian context. Many studies have been conducted to study the effect of input and of processes on performance such as innovation, information technology, human resource, technology, government regulation on performance of SMEs in a silo but, rarely all together. The qualitative analysis adds value to the research. Many of the outcomes of the research have been largely discussed in Indian print media which indicates the pragmatic approach of the research.
Defining and explicating value re-creation to solve marketplace problems for consumers with vulnerabilities
Authors: Zainuddin Nadia , Robinson Julia , Algie Jennifer , Randle Melanie 
Published: July 2021

PurposeThis paper aims to examine driving retirement and its impact on the well-being of older citizens. The concepts of value creation and destruction are used to understand older consumers’ experiences with the self-service consumption activity of driving. This paper formally introduces the concept of value re-creation, as a means of restoring the overall value lost from the destruction of certain components of previous value structures. In doing so, this paper explores the different ways that resources across the micro, meso and macro levels of the ecosystem can be re-aligned, in order for older citizens to maintain their well-being after driving retirement.Design/methodology/approachA qualitative, individual-depth interview approach was undertaken with 26 participants living in New South Wales, Australia. The participants comprised of both drivers approaching driving retirement age, as well as driving retirees. Thematic analysis was undertaken to analyse the data.FindingsThe findings identified that emotional value in the forms of freedom, independence/autonomy and enjoyment, functional value in the forms of convenience and mobility and community value are created from driving. Driving retirement destroys certain components of this value (e.g. enjoyment and convenience) irrevocably, however freedom, independence/autonomy, mobility and social connectedness can still be maintained through re-aligning resources across the micro, meso and macro levels of the ecosystem. New components of value are also created from driving retirement. These include peace of mind, which contributes to the re-creation of the emotional value dimension, and cost savings, which creates the new value dimension of economic value. These changes to the value structure effectively re-create the overall value obtained by individuals when they retire from driving.Originality/valueThe main contribution of this work is the formal introduction of the concept of value re-creation at the overall and value dimension level, and development of a conceptual model that explains how this value re-creation can occur. The model shows the resource contributions required across all levels of the ecosystem, expanding on existing conceptualisations that have predominantly focussed on resource contributions at the individual and service levels.
Factors influencing Palestinian small and medium enterprises intention to adopt mobile banking
Authors: Mujahed Hamed Mohammed Hamed , Musa Ahmed Elsadig , Samikon Siti Aida 
Published: July 2021

Purpose
This paper aims to examine the determinant factors that influence the adoption of mobile banking by small and medium enterprises (SMEs) in Palestine. The aim of this paper has supported with the objectives to identify the role of mobile banking practices in enhancing sustainable growth and development of Palestine SMEs; determining the factors that influencing the SMEs as a service and products providers’ intention to adopt mobile banking and proposing a conceptual model for adoption mobile banking development by SMEs sectors in Palestine

Design/methodology/approach
Hypotheses were developed guided by the technology organisation-environment (TOE) model. The primary data was collected from 408 SMEs in Palestine using questionnaires and 8 interviews.

Findings
The empirical results are based on partial least squares analysis and statistical package for the social sciences. The findings presented in this paper reveal that SMEs factors, enabling environment and business model are the main determinant factors affecting the intention to adopt mobile banking by the SMEs sector in Palestine.

Practical implications
The main contribution of this study is the concise identification of the obstacles and facilitators to SMEs’ mobile banking, especially in developing countries. A suggestion for further study was made. The findings could be useful to policymakers, government institutions and agencies especially in Palestine and other development partners in designing and directing their policy intervention.

Originality/value
This study contributes significantly to the theoretical understanding of mobile banking through the development of a mobile banking framework for the SMEs’ sector in Palestine. A major contribution of this study is to the existing knowledge and literature in mobile banking by developing a TOE framework for providers (supply-side) factors in general and in particular in Palestine to fill the gaps in past studies.
Cu(II)/sodium borohydride-based electrocatalytic system for reduction and dyeing of indigo
Authors: Li Xiaoyan , Zhang Zhihui , Yao Jiming , Wang MengQian , Yang Na 
Published: July 2021

Purpose
To improve the problems as the heavy burden of sewage treatment and environmental pollution caused by the traditional sodium hydrosulfite reduction dyeing of indigo, this study aims to carry out the direct electrochemical reduction dyeing for indigo with the eco-friendly Cu(II)/sodium borohydride reduction system under normal temperature and pressure conditions.

Design/methodology/approach
The electrochemical behavior of Cu(II)/sodium borohydride reduction system was investigated by cyclic voltammetry. And, the dyeing performance of the Cu(II)/sodium borohydride reduction system was developed by optimizing the concentration of copper sulfate in the anode electrolyte, applied voltage and reduction time via single-factor and orthogonal integrated analysis.

Findings
The dyeing performance of the Cu(II)/sodium borohydride reduction system is superior to that of the traditional reduction dyeing with sodium hydrosulfite. In the case of the optimized condition, the soaping fastness and dry/wet rubbing fastness of the dyed fabric in the two reduction dyeing processes were basically comparable, the K/S value of electrocatalytic reduction of indigo by Cu(II)/NaBH4 is 11.81, which is higher than that obtained by traditional sodium hydrosulfite reduction dyeing of indigo.

Originality/value
The innovative electrocatalytic reduction system applied herein uses sodium borohydride as the hydrogen source combined with Cu(II) complex as the catalyst, which can serve as a medium for electron transfer and active the dye molecule to make it easier to be reduced. The electrochemical dyeing strategy presented here provides a new idea to improve the reduction dyeing performance of indigo by sodium borohydride.