bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactococcus lactis and Lactococcus garvieae identified from Aceh buffalo (Bubalus bubalis) associated lactic acid bacterial isolates
Authors: Suhartono S , Misrahanum M , Yulvizar C , Khairami I R 
Published: March 2021

Abstract
Lactic acid bacteria (LAB) are one of the beneficial bacterial groups due to their metabolic activities, including production of bacteriocin that are potentially applied as biopreservation. The objectives of the study were to identify LAB isolate coded as BK-03 using biochemical and molecular approach as well as to determine the potency of the isolate in producing bacteriocin. The isolate of BK-03 recovered from the meat of Aceh indigenous buffalo was subjected to biochemical and molecular identification. Based on biochemical identification using the API 50 CHL, the LAB isolate of BK-03 was identified as Lactococcus lactis ssp lactis 1 with 98.6% proximity percentage. However, different results were obtained from molecular approach using 16S rRNA, as the isolate was identified as Lactococcus garvieae with a a similarity index of 99%. The results of the antimicrobial assay showed that the crude extracts of BK-03 bacteriocin did not show an inhibition zone to Escherichia coli, but had an inhibitory zone against Staphylococcus aureus with a average diameter of 7.45 mm.
Water quality characteristics and modeling of Tasik Aman, USM, Penang
Authors: Munirah Z M , Muhammad Izzat Emir K B , Wan Maznah W O , Teh S Y , Ismail A H , Eshak M B 
Published: March 2021

Abstract

Tasik Aman was constructed in 1990 as a flood retention pond in the Universiti Sains Malaysia, Main Campus, Penang, Malaysia. Tasik Aman has been experiencing accumulation of excess nutrients from the surrounding area mainly from Sungai Gambir, causing high algae population in the water body. Thus, a study was carried out in December 2019, focusing on the current water quality determination of Tasik Aman, to predict the future water quality condition of the lake. Four sites were designated to conduct water quality analysis comprising of water temperature, dissolved oxygen (DO), pH, transparency, biological oxygen demand (BOD), total dissolved solids, total suspended solids, conductivity, orthophosphate, chlorophyll-a, total phosphorus, ammonia, nitrite, and nitrate. Some physico-chemical parameters were measured in-situ while some were measured in the lab by analyzing the water samples collected in the field in accordance with APHA (2005). DO and BOD data were modeled using Water Quality Analysis Simulation Program (WASP). Tasik Aman showed high readings in temperature, TSS, TDS, conductivity, DO, BOD, chlorophyll-a, and nitrate-nitrogen. One-way ANOVA further revealed significant differences of DO, TDS, conductivity, transparency, orthophosphate, nitrate, and chlorophyll-a among the stations of Tasik Aman (p<0.05). Based on WASP analysis, simulated graphs produced for Tasik Aman showed a decrease in DO and an increase in BOD level proving that the lake contain high nutrient content that may exacerbate the eutrophication.
Effect of different nitrogen sources on cell growth and biochemical compositions of Chlorococcum sp. cultivated under laboratory conditions
Authors: Fatini M A , Basri E M , Wan Maznah W O 
Published: March 2021

Abstract
Different sources of nitrogen can affect the growth and biochemical composition of microalgae. The present study was conducted to determine the effect of different nitrogen sources on algal growth, carbohydrate and protein contents, of freshwater microalgae Chlorococcum sp. Three different nitrogen sources were used in this study including sodium nitrate, ammonium chloride and urea. The cultivation of the algae of different nitrogen sources took place in the same parameter conditions (24 hours of light duration; 25 ± 1 °C of temperature; 7.20 ± 0.02 of pH). The algal cells were harvested at the early stationary phase for biochemical analysis. The number of cells were quantified by using a counting chamber (haemocytometer) and growth curves were plotted to determine the growth rate. The results revealed that Chlorococcum sp. cultivated in urea as the nitrogen source exhibited the highest growth rate. Apart from that, it was also found that the cells cultivated in urea displayed the highest carbohydrate and protein content as compared to the other two nitrogen sources which were 25.5% and 18.7%, respectively. Moreover, the cells cultivated in urea were medium in size during early stationary phase indicating urea the best nitrogen source for Chlorococcum sp. under controlled conditions.
The diversity of echinoderms in the seagrass meadows of Penang Island, Malaysia
Authors: Teoh V Y J , Woo S P 
Published: March 2021

Abstract
Many studies had been done on the diversity of echinoderms in Malaysia, especially on holothuroids, but only a handful was conducted on seagrass meadows. The lack of studies conducted on echinoderms in seagrass meadows coupled with the steadily declining area of seagrass meadows represents a serious threat to the documentation of Malaysia’s echinoderm biodiversity. This study was conducted to provide a checklist of echinoderms found in the seagrass meadows of Penang Island. Four sampling trips were conducted from October 2019 to February 2020 and specimens were collected by hand during low tide in two sites, Pulau Gazumbo and Middle Bank. Additional specimens in the collection of Centre for Marine and Coastal Studies, Universiti Sains Malaysia (CEMACS, USM) were also included in this study. Ossicles of holothuroids were extracted, observed under a light microscope, and illustrated. A total of 31 specimens were examined and identified, comprised of 15 species: one from the class Asteroidea; seven from the class Ophiuroidea; two from the class Echinoidea; and five from the class Holothuroidea. The results would be able to serve as a basis for future studies on the taxonomy, diversity, and distribution of echinoderms in the seagrass meadows of Malaysia.
The detoxification enzymes activity profile in susceptible Aedes and Culex mosquitoes
Authors: Farouk S A , Barahim N , Hamzah S N 
Published: March 2021

Abstract
The status of susceptible mosquito is important for monitoring and managing insecticide resistance in field population. The purpose of this study is to profile the differential expression level of enzymatic activities of metabolic enzymes exhibited in the mosquito species of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus from the susceptible strain. The fourth instar larvae of each strain were subjected to biochemical assay. The total protein content and enzymatic activities of Glutathione S-transferase (GST), α-esterase (α-est), β-esterase (β-est) Cytochrome P450 (Cyt P450) and Acetylcholinesterase (AChE) from each mosquito strains were elucidated. Significant difference (P<0.05) was detected between the total protein content between all species of susceptible strain mosquitoes. One-way ANOVA showed that the specific enzymatic activities of GST, α-est and Cyt P450 of all test mosquitoes were significantly different upon comparison with each species (P<0.05). The mean of enzymatic activities of insensitive AChE showed no significant difference upon comparison with each other (P>0.05). The enzymatic activities of β-est shows no significant difference between Ae. aegypti and Cx. quinquefasciatus mosquitoes (P>0.05) but the enzymatic activity of β-est in Ae. albopictus manifested significant difference upon comparison with the enzymatic activities of the other two test species (P<0.05). The results obtained may provide more information about the enzymatic activities of metabolic enzymes in Ae. aegypti, Ae. albopictus and Cx. quinquefasciatus mosquitoes which might be beneficial for public sector for the application of proper vector control measures.
Potato tuber (Solanum tuberosum L.) formation due to the application of different concentrations of coconut water in in-vitro
Authors: Sembiring R , Hayati M , Kesumawti E 
Published: March 2021

Abstract
This study apply coconut water to support the process of formation of potato tubers in vitro. The study was conducted at the laboratory of tissue culture, Experimental Garden of Vegetable Research Center, Berastagi, North Sumatera. The experiment used Completely Randomized Design with single treatment factor, the coconut water concentration of 0; 75; 150 and 225 ml L−1. The results show that the application of coconut water provides a significant effect on the number, diameter and fresh weight of potato micro tubers, but the effect is not significant at the time of potato micro tuber formation. The application concentration of coconut water 225 mL L−1 affected the potato micro tuber formation in in vitro.
Characterization of mechanical properties polypropylene composites film filled with corncob as reinforcement
Authors: Gunawan A , Pasaribu H , Lauzia T , Syamsuddin Y , Meiriza L , Kamaruzaman S , Aprilia S 
Published: March 2021

Abstract
The use of materials in cars has changed significantly over the last few decades. Polypropylene is the top choice for automotive industry players because it has unparalleled flexibility, affordable cost and performance balance that successfully replace steel and other plastics as the material for the manufacture of motor vehicles. However, since the raw material of polypropylene is derived entirely from petroleum and natural gas which can be discharged at any time. Also, its use is not environmentally friendly which need an action to overcome one of them by developing composite technology. The purpose of this study was to know the relation of mass fraction of corncob fiber into polypropylene composites on tensile strength and composite morphology. Corncobs reinforce in polypropylene are 10, 20, 30 and 40% by weight. XRD analysis investigated at 2θ the crystalline part of corncob at 21°. The functional group of Si-OH and Si-O-Si were analyzed at wavelength 1030 and 1100 cm−1. The fracture surface of composite shows the crack effect of particle loading. Polypropylene composites filled with corncob increased in tensile strength and decrease in elongation at break with the increase of corncob particle loading.
The potential of Trichoderma species to remediate silver nanoparticles contamination
Authors: Oktarina H , Artika W , Mukhriza T 
Published: March 2021

Abstract
Trichoderma is one of soil fungi that has been widely explored for the removal and recovery of heavy metals. A preliminary experiment was set to investigate the potential of two local species of Trichoderma, T. harzianum and T. virens, to remediate silver nanoparticles (AgNPs) contamination. AgNPs was studied as they have the most commercial applications as consumer products and accumulated in soil. Four levels of AgNPs (0, 100,200,300 mg L−1) were used to examine the ability of each species to grow in contaminated media. The growth of Trichoderma spp. was observed by measuring the colony diameter and spore production. The results showed that T. harzianum was more sensitive to AgNPs than T. virens as the colony diameter and spore production reduced significantly. The study indicates that T. virens has a potential to remediate AgNPs contamination.
The effect of yeast and lactic acid bacteria as probiotic on the total of Vibrio spp. in rearing water of post larvae tiger shrimp (Penaeus monodon)
Authors: Nurliana  , Sutriana A , Azhar A , Erina  , Jalaluddin M , Andista B K 
Published: March 2021

Abstract
Probiotics are an alternative that can be used to prevent the incidence of vibriosis in the cultivation of tiger shrimp (Penaeus monodon). Yeast can be used as a probiotic and immunostimulant because it can increase the total of beneficial microbes. Lactic Acid Bacteria (LAB) are chosen to be developed as a probiotic because it is able to produce organic acids, hydrogen peroxide and bacteriocin which act as antagonistic agent to Vibrio spp. This study aimed to determine the effect of RABAL probiotics on the total Vibrio spp. in medium of post larvae tiger shrimp (Penaeus monodon). The study was conducted with an experimental method using a completely randomized design (CRD) with 4 treatment groups and 3 repetitions. Calculate of the total amount of Vibrio spp. and LAB on rearing water was carried out using Total Plate Total (TPC) method. Based on the results of the study, it was found that administering RABAL probiotics with a dose range of 2,5 to 7,6 ml / L was proven to reduce the amount of Vibrio spp. in rearing water of post larvae tiger shrimp (Penaeus monodon).
Reinforcement method of reinforced concrete beam by using non-stressing strand
Authors: Susanto A , Kasyanto H , Susilahadi S 
Published: March 2021

Abstract
Many methods can be used in the repair of bridges or other structural buildings. The aim is to increase the existing capacity. In the reinforced concrete beam structure, capacity of structural elements can be carried out by reinforcement method. The research was done by experimental loading test in laboratory. The test objects are consisted of 2 (two) reinforced concrete beams: a normal beam without reinforcement (NB) and a reinforcement beam by using non-stressing srand (RB1). The dimensions of the beams are 150mm x 300mm x 3200 mm. All beams are loaded by static-monotonic load until collapses. From the results of the research, the first yield load for the NB beam and RB1 beam respectively are 22 kN and 29kN. The deformations that occur at the conditions are 9.5mm and 14mm respectively. The collapse occurring on the NB beam is the bending collapse, whereas in the RB1 beam is a bending- shear collapse.
Performance improvement of Asphalt Concrete-Wearing Course with modification of masterbatch SIR20
Authors: Yulianti N P , Sulaiman S , Utami R 
Published: March 2021

Abstract

Asphalt Concrete-Wearing Course is a layer that is located at the top of flexible pavement structure. Types of damage that often occur in the AC-WC layer are rutting and slippery. AC-WC modification with masterbatch SIR20 is a system that improves AC-WC performance in reducing deformation in pavement structure and improving crack and skid resistance. The method that is used in this study is an experimental method that compares and analyzes the performance of the modified AC-WC layer with variations of masterbatch content are 2%, 4%, 6% and 8% and 0% as a control. The 4% of masterbatch content in the AC-WC modification layer is an effective and efficient option to be applied as a surface layer of a pavement structure.
Prediction of sediments discharge in watershed with two tank models
Authors: Mulyono T , Jumi J , Azizah A 
Published: March 2021

Abstract
Uncontrolled erosion would cause considerable damages, such as soil fertility decline, water structures damage and reservoirs sedimentation. As the data for the sedimentation rate are limited, several models have been developed to predict the surface erosion and the rate of sedimentation. However, the availability of sufficient, diverse and extensive data is needed for the implementation of the models, both for the model calibration and the verification. The result of the analysis shows that both of the Water Tank Models that represent the erosion-sedimentation rate process, in which Water Tank 1 being the three-tank cascade system and Water Tank 2 being two-tank cascade system, are not optimum. This can be observed from the values of volume error (VE), relative error (RE), root-mean-square error (RMSE) and correlation coefficient (R) that show the effect of 1.5 hours of rain period in the sedimentation rate. The field condition shows considerable sedimentation, on the other hand, the models’ simulations show decreasing sedimentation rates. The optimum model’s parameters for Water Tank 1 and Water Tank 2 are 924.51%-1049.26% for the relative error, 50.81% - 121.42% for the volume error, 0.9 for the correlation coefficient and 6703.59-17,297.85 for the root-mean-square error. The parameters and constant’s values of the models are different relative to the drainage basins’ condition.
VeRO: Smart home assistant for blind with voice recognition
Authors: Triyono L , Yudantoro T R , Sukamto S , Hestinigsih I 
Published: March 2021

Abstract
In daily life, people with visual impairment have difficulty in their activities, especially at home activities. People with visual impairment as of 2017 reached 1.5 percent or around 3.75 million people from the population of Indonesia and more than 253 million people worldwide. The number of blind people who are not small need help to get a good quality of life and independent. Friendly home design for the blind is not yet available specifically. It is a particular difficulty for blind people to access the latest electronic goods or information (news). Then this becomes the basis of this research on how to provide easy access for blind people. This research provides a solution that is designing a prototype of a house that is visually impaired in addition to the use of electronic equipment. This system starts from the microphone as a sensor in charge of picking up sound and then the results are processed by Android and then connected to NodeMCU via Wi-Fi. The microphone used is the default microphone of the Android smart phone. By using the speech recognition method, the command is enough to say the keywords, Android will manage the sound into a code that will be sent to the microcontroller to perform the task. There are several categories of commands that can be handled, including (a) Turning on / off electronic devices, (b) Providing news information from popular news web portals, (c) Entertainment by playing streaming radio and (d) General information on hours and dates. All planned functions are running properly, there are some features that might be developed further. Adding sensitivity to starting voice commands and adding the ability to read long stories can be further research.
Online monitoring system for emergency diabetes mellitus patients
Authors: Kurniawati R , Suryono S , Ismail S 
Published: March 2021

Abstract
In this research, an online monitoring system was designed to detect emergency due to uncontrolled blood glucose fluctuations in patients with diabetes mellitus. Hyperglycemia in the morning causes dawn phenomenon and Somogyi effect. This incident is very fast, and its uncontrollable time of occurrence can inflict a fatal condition causing death. Unfortunately, there are no systems able to detect such events, currently. This study proposes an online monitoring system with the input of the patient’s blood glucose measurement into the smartphone. Blood glucose values are sent and stored in the webserver, and the results are used to determine the incidence of dawn phenomenon and the Somogyi effect. The study was able to produce an online monitoring system which can be monitored by nurses, doctors, and administrators via the dashboard.
Survey of smart parking application deployment
Authors: Nugraha D , Ahmed F Y H , Abdullah M I , Johar M G M 
Published: March 2021

Abstract
Number of vehicles continues to increase in the urban place parallel with the infrastructure advancement. The increase of vehicles on road causes various problems and obstacles to find an empty parking slot. This problem often causes congestion, waste more effort and time-consuming process especially during rush hours when many people are driving for various reasons. In this paper, we explore the concept of smart parking based on the application of their categories and explain the classification from various parking support technologies. Present developed parking system supported by various technologies and the category. Classified the application function of this survey. The application of this smart parking application might assist in reducing congestion and other issue by manage the parking process. Achieve the key objective of this research as stated by various scientist and researcher to deliver an advanced parking system. Conducted by developing an application interface base on a mobile, desktop and web. It is an excellent determination for the parking management to deliver a real-time information update on the parking area, which is the point of interest as an improvement to the existing system and research.
QoS analysis of WSN (Wireless Sensor Network) using node MCU and accelerometer sensors on bridge monitoring systems
Authors: Junesco D , Supriyanto E , Hasan A , Mukhlisin M 
Published: March 2021

Abstract
Based on the planning guideline of Health Monitoring System of Bridge Structure of Kementrian Pekerjaan Umum dan Perumahan Rakyat, one of the main components needed in bridge health monitoring system is a communication system. The application of the WSN (Wireless Sensor Network) function to perform monitoring can be used to create a more practical system. Based on the system, required hardware that can be integrated with the software and can connect on wireless. Proposed methods using WSN (Wireless Sensor Network) on devices, then do testing on connectivity to obtain network quality and data sensor. Data sensor will be uploaded on the cloud can be accessed from various places that connected to the Internet network thus forming an IoT (Internet of Things) based system with using Message Queue Telemetry Transport (MQTT) protocol. Experiments carried out in two ways of connectivity, point-to-point and star topology to determine the network quality of each device, which is Node MCU and Mi-Fi modems. Network quality parameters can be known by looking at the value of packet loss, delay, jitter, and throughput. In the results, the MQTT Protocol applied to build IoT system. In addition, proposed methods that used in this research has a great quality network with packet loss <25 ms. However, there’s a big gap between serial data and online data that makes MQTT protocol is not suitable for monitoring systems that require fast data transfer.
Double security system with RFID and password based on Arduino Mega 2560 and PLC
Authors: Syahid S , Supriyatna S , Badruzzaman Y , Antono D , Santoso A 
Published: March 2021

Abstract
This research focuses on the use of RFID (Radio Frequency Identification) systems that are used as personal identification cards in the room access system. This system is intended to maintain the security and privacy of the room from someone who does not have the authority to enter the room. This security system uses a two-tier security system, namely through an RFID identity card and security code. The design and implementation of this system, an electric key work system is evaluated based on the solenoid component and the password used when accessing the room. RFID tags used in this system are in the form of “Mifare RFID Card 13.56 Mhz” cards and store unique codes used as personal identification. This code is read by the RFID reader in validating its authority with Arduino Mega 2560 to regulate the system of electric locks and passwords used to open the door. From the test results, 100% RFID tags can be recognized by the RFID reader, 100% RFID tag and password reading system is successfully used to access the room.