bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Differentially Expressed Genes in Osteonecrosis of Femoral Head, Osteoarthritis, and Rheumatoid Arthritis by Integrated Microarray Analysis
Authors: zhao gang , liu yujie , li zhongli , li chunbao 
Published: October 2021

Abstract
BackgroundOsteonecrosis of femoral head (ONFH), osteoarthritis (OA) and rheumatoid arthritis (RA) are common diseases of hip joint, which will damage the joint to varying degrees, and will affect the mobility and quality of life of patients in severe cases. A better understanding of the expression of articular cartilage degeneration genes seems to be very important for further understanding the molecular mechanism of the joint action of the three diseases.Objectivethe purpose of this study was to explore the pathogenesis of cartilage injury in three diseases by analyzing the data sets of multiple gene expression groups and synthesizing the differentially expressed genes (DEGs) of ONFH, OA and RA.Methodsthe gene expression datasets of ONFH, OA and RA were obtained from Gene Expression Omnibus. Through the comprehensive analysis of multiple gene expression data sets, the common differentially expressed genes and specific DEGs in these diseases were found. Gene ontology (GO) analysis, KEGG pathway and protein-protein interaction analysis were used to investigate the functions of the altered proteins and biological pathways.ResultsA total of 59 common differentially expressed genes were obtained by GSE74089 and GSE55235 data sets, including 44 up-regulated genes and 15 down-regulated genes. GO and KEGG pathway enrichment analysis showed that DEGs concentrated on extracellular structure organization, extracellular matrix organization, fibrillar collagen trimer, platelet-derived growth factor binding, Relaxin signaling pathway, Protein digestion and absorption. Some hub genes with high interactions such as COL1A2, MMP1, VEGFC, SPP1, etc. Independent GEO dataset verification results show that these genes are consistent with the experimental results.ConclusionIn this study, bioinformatics methods were used to identify the common differential genes of ONFH, OA and RA, and to analyze the occurrence and development of cartilage degeneration or osteogenic diseases induced by them. To provide molecular basis for follow-up experiments and clinical efficacy.
A Prospective Clinico-Epidemiological Evaluation and Management of Hump Nosed Pit Vipers (Hypnale Hypnale) Bite Envenoming in Dogs
Authors: Adhikari Ranjith , Silva Niranjala , Dangolla Ashoka , Suriyagoda Lalith , Premarathna Amal D , Tuvikene Rando , Mallawa Chandima , Silva Indira , Gawarammana Indika 
Published: October 2021

Abstract
Human envenoming from the bite of the abundant hump nosed pit viper (Hypnale hypnale) (HNPV) is a frequent occurrence with victims experiencing unpleasant and sometimes life-threatening consequences. Regrettably, our therapeutic armamentarium remains limited. Further, clinico-pathology, treatment and management measures in HNPV envenomed dogs are under recognized. Prospective investigations were performed to assess the clilinico-pathology and management options for HNPV envenomed dogs brought to the University of Peradeniya’s Veterinary Teaching Hospital from January, 2012 to March 2018. We recorded the local and systemic clinical manifestations, hematological and urinary abnormalities of 78 dogs in which HNPV bite had been witnessed by the owner. Mild swelling, extensive swelling, hemorrhagic blistering and hemorrhagic bullae at the site of bite were observed in 59%, 31%, 6% and 4% of the dogs, respectively. Some dogs were subjected to surgical excision of necrotized tissue including limb amputation. We observed the following systemic clinical effects in envenomed dogs: neurotoxicity (13%), acute kidney injuries (AKI) (14%) and coagulopathy (16%). All dogs showed leukocytosis with mean white blood cell count of 25.25×103/µL. Mild anemia and thrombocytopenia were detected in 29% of the dogs. There was a significant correlation between extent of local tissue injuries with length of hospitalization (LH). The mean time of coagulopathy observed was 21.3 hours (IQR: 8-48 hours). In coagulopathic dogs, there was a strong correlation between LH and extent of local tissue injury (rs = 0.7751, P < 0.0001); LH and clotting time (rs = 1.0, P < 0.0001); PT and aPTT (rs = 0.4712, P < 0.001). LH was significantly correlated with the development of AKI (p=0.0013). Lack of specific AVS for HNPV envenoming provided an opportunity to study the remaining treatment options. Therefore, the study allowed the identification of local and systemic effects, hematological abnormalities, possible auxiliary treatments and drawbacks of management measures for envenomed dogs.
Value of Biomarkers in Liver Cancer EMT model under different interventions：A meta-analysis
Authors: Yan Jing , Zou Shuli , Xie Bei , Tian Ye , Peng Zhiheng , Liu Zhuan , Ma Bin , Li Linjing 
Published: October 2021

Abstract
Background

There are various interventions to establish the Liver cancer epithelial-mesenchymal transition (EMT) models. However, the ideal biomarkers for unique model are not well established. Further studies are necessary to evaluation of effective EMT biomarkers under different interventions in vitro studies. A meta-analysis was performed to evaluate the performance of different biomarkers in HepG2 cells during EMT under multiple interventions.
Methods

PubMed, Web of Science, Embase, the China National Knowledge Infrastructure (CNKI), the China Biology Medicine disc (CBM), Wan Fang Data, and VIP databases were systematically searched from inception to June 14, 2020 by two independent reviewers.
Results

A total of 58 studies were included in the meta-analysis. Our study showed that E-cadherin responds well to the intervention of medication, genetic intervention, gene knockout/knockdown, hypoxia, and other tumor microenvironments, as well as non-coding RNA (ncRNA) overexpression and silencing. N-cadherin can effectively evaluate the intervention effect of medication, genetic intervention, hypoxia and other tumor microenvironments, as well as ncRNA overexpression. Vimentin reflects the effects of medication, pro-EMT genetic intervention and gene knockout/knockdown, anti-EMT ncRNA overexpression and anti-EMT ncRNA silencing and hypoxia. Snail only responds to the intervention of anti-EMT genetic intervention and gene knockout/knockdown, tumor microenvironments other than hypoxia, anti-EMT ncRNA overexpression and ncRNA silencing.
Conclusions

Our results shows that some medicine, some gene, microenvironment and some ncRNA can effectively induce/inhibit EMT process. E-cadherin, N-cadherin, Vimentin and Snail are effective biomarkers during this process. They respond differently to different intervention. Therefore, different biomarkers should be chosen under different intervention based on their performance.
Epitaxial Growth and Optical Properties of Laser Deposited CdS Thin Films
Authors: Al-shamiri Hamdan Ali Sultan Ali Sultan , Gadalla Atef S. , Khalil Huda F. , Nahas Mahmoud M. El , Khedr Mohamed A. 
Published: October 2021

Abstract
Cadmium Sulfide (Cds) thin films were synthesized on quartz substrates using infrared pulsed laser deposition (IR-PLD) technique under high vacuum (~ 10− 6 Torr). X-ray diffraction was used to evaluate the structural features. According to X-ray analysis the deposited CdS films are crystalline and have a favored orientation on a plane (110) of an orthorhombic system and the peak intensity and the average crystallite size increases with increasing the film thickness. After annealing at 300oC the orthorhombic phase transformed into predominant hexagonal phase and the same result was obtained by SEM photographs. Spectrophotometric measurements of transmittance and reflectance of the Cds films were used to derive optical constants (n, k and absorption coefficient α). The optical band gap energy was found to be 2.44 eV. The plasma plume formation and expansion during the film deposition have been discussed. The photocurrent response as a function of the incident photon energy E (eV) at different bias voltages for different samples of thicknesses (85, 180, 220 and 340 nm) have been studied, indicating that the photocurrent increases by increasing both the film thickness and photon energy with a peak in the vicinity of the band edge. Thus, the prepared Cds films are promising for application in optoelectronic field.
Subsurface Soil Carbon and Nitrogen Losses Offset Surface Carbon Accumulation in Abandoned Agricultural Fields
Authors: Yang Yi , Knops Johannes M. H. 
Published: October 2021

Abstract
Abandoned agricultural fields (old fields) are thought to accumulate soil organic matter (SOM) after cultivation cessation. However, most research on old fields soil carbon (C) and nitrogen (N) sequestration has focused on the surface (10 to 20 cm) and overlooked their dynamics below 20 cm. This study aimed to quantify C and N stock change in both surface and subsurface soil in old fields and examine the mechanisms underlying the observed dynamics. We conducted 13-year long-term surveys of 21 old fields at Cedar Creek Ecosystem Science Reserve (CCESR), Minnesota, and measured inputs and outputs of organic matter at different depths, including above- and belowground biomass, SOM decomposition, and dissolved organic C (DOC). Soil C and N accumulated by 30.5 ± 6.5 g C m⁻² yr⁻¹ and 2.41 ± 0.49 g N m⁻² yr⁻¹ in the surface (0–20 cm) yet decreased by 64.6 ± 12.5 g C m⁻² yr⁻¹ and 6.83 ± 1.34 g N m⁻² yr⁻¹ in the subsurface (20–100 cm). The C and N losses in the subsurface soil were correlated with low deep root biomass, the majority of roots are located in the top 20 cm soil. Such root distribution can be attributed to the continuing dominance of nonnative and shallow-rooted C3 grasses and the lack of legumes after abandonment. This study showed current old field biogeochemistry and vegetation composition are fundamentally different from the pre-settlement grasslands, indicating the agricultural abandonment has long legacy effects on subsurface soil C and N.
Study of Anterior Capsular Contraction Following Cataract Surgery With Frequently Used Intraocular Lenses
Authors: Zhou Yongmou , Ding Yinghui , Huang Yan , Hou Ping 
Published: October 2021

Abstract
Purpose: Anterior capsular contraction syndrome (ACCS) was compared in this study with cataract patients whose eyes were implanted with one of two types of acrylic lenses frequently used.Methods: The study included 1305 eyes of 1278 patients.All of them phacoemulsification and intraocular lens(IOL) implantation were successfully performed. Each patient was operated on by the same surgeon，Two types of IOLs were implantation during cataract surgery: Tecnis ZCB00 [Johnson & Johnson Vision] (IOL A), the Akreos AO [Bausch & Lomb]) (IOL B).Patients with intraoperative capsular tears and follow-up survey less than 1 month were excluded. In these cases, we recorded the duration of postoperative follow-up, the presence of ACCS, along with the dimensions of the anterior capsule opening. A comparison of ACCS incidence was compared between the two lenses.Results: Although the ZCB00 group had more patients with ACCS risk factors (P, 0.009), the ZCB00 group had a significantly lower incidence of ACCS than the Akreos AO group (P=0.032). Conclusion: Compared with the Akreos AO (IOL B), the ZCB00 (IOL A) showed better anterior capsular stability and a lower incidence of ACCS.
Genome-Wide Identification of The NHXs in Rosa Multiflora Reveals a Key Role of RmNHX2 in Salt Tolerance
Authors: Ping Luo , Shen Yuxiao , Chen Linmei , Chen Wen , Cui Yongyi 
Published: October 2021

Abstract
High salinity restricts plant growth and geographic distribution. Plant intracellular Na+/H+ (NHX) antiporters have critical roles in plant development and stress response. However, the molecular functions of RmNHXs in Rosa multiflora remain obscure. In this study, we identified 11 putative RmNHXs in Rosa multiflora according to the genome-wide analysis. The RmNHX genes were classified into 3 classes. Most of the RmNHX genes were responsive to salt stress, with the greatest up-regulation being observed in RmNHX2. RmNHX2 was localized at the tonoplast. RmNHX2 overexpression resulted in the enhanced salt tolerance in tobacco, whereas virus-induced gene silencing (VIGS) of RmNHX2 in R.multiflora increasedsalt susceptibility. Under salt treatment, the transgenic tobacco plants achieved less reactive oxygen species (ROS) accumulation and higher activities of enzymes (e.g., superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)), which complied with the up-regulated expressions of antioxidant genes. In addition, RmNHX2-overexpression lines had a lower level of Na+, a higher level of K+, as well as a lower Na/K ratio. In contrast to the mentioned, VIGS of RmNHX2 in R.multiflora exhibited the opposite phenotype, accompanied by compromised salt tolerance. RmNHX2 enhances plant salt tolerance by maintaining proper ion homeostasis, as well as by accelerating ROS scavenging. For this reason, RmNHX2 has great potential to be employed for the engineering of ornamental plants in terms of enhanced salt tolerance subsequently.
The Cross-Industry Universal Laws of Exponential Booking Curves
Authors: Shintani Masaru , Umeno Ken 
Published: October 2021

Abstract
In recent years, the e-commerce market has grown with the spread of the internet worldwide every year. Accordingly, in service industries, purchasing products with reservations has become common. With the spread of online reservations, the booking curve, which is the concept of the time series in the cumulative number of reservations and has been used for sales optimization in the airline ticket and hotel industries, has been used in various industries. Booking curves in specific industries have been studied, but a universally applicable model across various industries has not been developed. In this study, we show that booking curves can be modeled universally by the exponential decay function, and we also show that the model is valid by using real data from some industries before and after the COVID-19 pandemic, that is, under completely different market conditions. The cross-industry exponential laws of booking curves constitute an important discovery in regard to mathematical laws in the social sciences and can be applied to give leading microeconomic indicators.
Multiplexed Complementary Signal Transmission for a Self-Regulating Artificial Nervous System
Authors: Choi Young Jin , Roe Dong Gue , Choi Yoon Young , Kim Seongchan , Jo Sae Byeok , Lee Hwa Sung , Kim Do Hwan , Cho Jeong Ho 
Published: October 2021

Abstract
Neuromorphic engineering has emerged as a promising research field that can enable efficient and sophisticated signal transmission by mimicking the biological nervous system. This paper presents an artificial nervous system capable of facile self-regulation via multiplexed complementary signals. Based on the tunable nature of the Schottky barrier of a complementary signal integration circuit, a pair of complementary signals was successfully integrated to realize efficient signal transmission. As a proof of concept, a feedback-based blood glucose level control system was constructed by incorporating a glucose/insulin sensor, a complementary signal integration circuit, an artificial synapse, and an artificial neuron circuit. Certain amounts of glucose and insulin in the initial state were detected by each sensor and reflected as positive and negative amplitudes of the multiplexed presynaptic pulses, respectively. Subsequently, the pulses were converted to postsynaptic current, which triggered the injection of glucose or insulin in a way that confined the glucose level to a desirable range. The proposed artificial nervous system demonstrates the notable potential of practical advances in complementary control engineering.
A Regime Shift in the Interhemispheric Teleconnection between the Yellow and East China Seas and The Southeastern Tropical Pacific in The Southern Hemisphere during The Boreal Summer
Authors: Kim Yong Sun , Kwon Minho , Chung Eui-Seok , Yeh Sang-Wook , Jeong Jin-Yong , Jang Chan Joo 
Published: October 2021

Abstract
Through statistical estimations on reconstructed datasets for the period 1982−2020 after removing a long-term trend, we observed that there was a drastic regime shift in the early summer’s connection between the YECS and the tropical Pacific in the early 2000s. The summer YECS SSTs had seemed to be modulated by local oceanic and atmospheric processes along with their marginal coupling to the tropical Pacific during the pre-2003 period before the regime shift. In contrast, an interhemispheric YECS−tropical southeastern Pacific (SEP) coupling appeared after the regime shift. This teleconnection was at least partially attributed to a reduced El Niño signature in the tropical Pacific, which favors the emergence of the South Pacific meridional mode (SPMM) independently from ENSO signals. Precipitation anomalies in the western tropical Pacific act as an atmospheric bridge to mediate the air-sea interacted variability associated with the SPMM into the North Pacific. The susceptibility of the YECS to atmospheric forcing may highlight the role of SST over the YECS as a potential indicator of basin-scale climate changes.
Impact of Cone-Beam Computed Tomography with Automated Feeder Detection Software on the Survival Outcome of Patients with Hepatocellular Carcinoma During Chemoembolization
Authors: Bannangkoon Kitttipitch , Hongsakul Keerati , Tubtawee Teeravut 
Published: October 2021

Abstract
Background

Inoperable hepatocellular carcinoma (HCC) is treated by transarterial chemoembolization (TACE) using cone-beam computed tomography (CBCT) or digital subtraction angiography (DSA). We compared patient survival outcomes between CBCT-TACE with automated tumor-feeder detection (AFD) software and DSA-TACE alone in inoperable HCC patients.
Methods

We reviewed the data of 337 HCC patients treated by CBCT-TACE or DSA-TACE between January 2015 and December 2019. Treatment response, progression-free survival (PFS), overall survival (OS), and complications between the CBCT-TACE and DSA-TACE groups were compared. Univariate and multivariate logistic regression analyses examined the potential prognostic factors affecting survival after chemoembolization.
Results

Tumor response rates in complete response, partial response, and stable disease at 1 month were 67%, 28%, and 4% in the CBCT-TACE group and 22%, 48%, and 9% in the DSA-TACE group, respectively. OS rates of patients in the CBCT-TACE vs DSA-TACE groups were 87% vs. 54%, 44% vs. 15%, and 34% vs. 7% at 1, 3, and 5 years, respectively. The CBCT-TACE group had significantly improved PFS (p < 0.001) and OS (p < 0.001). Multivariate analysis showed that CBCT with AFD software was an independent factor associated with longer OS (hazard ratio, 0.38; p < 0.001).
Conclusions

Compared with conventional DSA, combining selective TACE with CBCT and AFD software leads to better tumor response and prolongs OS in patients with inoperable HCC.
miR166h Directed Cleavage of Target Upon PGPR Inoculation Under Drought Stress and Tissue-Specific Expression Analysis Under Abiotic Stresses in Chickpea.
Authors: Yadav Ankita , Kumar Sanoj , Verma Rita , Rai Shashi Pandey , Lata Charu , Sanyal Indraneel 
Published: October 2021

Abstract
Legumes are an indispensable food after cereals with extensive production across the world. The legume production is imposed with limitations and has been augmented by various environmental stresses. The symbiotic relations between legumes and rhizobacteria have been an intriguing topic of research in view of their roles in plant growth, development and various stress responses. Recent advances on gene networks involving plethora of evolutionarily conserved miRNAs have been investigated pertaining to their roles in plant stress responses. The interaction between plant growth promoting rhizobacteria (PGPR) strain Pseudomonas putida RA, MTCC5279 and abiotic stress responsive miRNAs have previously been studied with roles in abiotic stress mitigation by modulating stress responsive miRNAs and their target genes. The present studyis an investigation involving the role of RA in abiotic stress responsive miR166h for drought mitigation in tolerant desi chickpea genotype. miRNA166 directed cleavage of its target, ATHB15 has been drifted of drought treated plantlets upon RA inoculation using 5´RLM-RACE analysis. Drought stressed chickpea plants when inoculated with growth promoting rhizobacteria, RA, the inverse correlation in expression patterns were noticed in miR166h and its validated target, ATHB15. Tissue-specific expression patterns in 15 days old chickpea seedlings including leaves, shoot and roots when exposed to salinity, drought and abscisic acid at different time points indicated the role of miR166 in different abiotic stress response. In view of the results, validation and functional characterization of such interactions involving stress responsive miRNAs along with microbial stress management techniques could be an important technique for crop improvement.
An Exploration of Midwives’ Experiences of Reducing Maternal Morbidity and Mortality From Postpartum Haemorrhage (PPH) in Rural Communities in Eastern Nigeria.
Authors: Kalu Felicity Agwu , Chukwurah Joan Nkechi 
Published: October 2021

Abstract
Background

Postpartum haemorrhage (PPH) is one of the major complications of childbirth which results in maternal morbidity and mortality especially in low and middle-income countries like Nigeria. Midwives play a vital role in preventing and managing PPH in Nigerian rural communities. The aim of this study is to understand the experiences of midwives in rural maternity care settings in order to provide appropriate support and improve practice.
Methods

An exploratory qualitative study of a purposive sample of 15 practicing midwives was carried out using semi-structured interviews from November 2018 to February 2019. Data were transcribed verbatim and analyzed using content analysis.
Results

Four themes were identified; intervention for preventing PPH, approaches to managing PPH, challenges of preventing and managing PPH, and ways of supporting midwives to overcome the challenges of preventing and managing PPH in rural health care settings. Midwives employed various strategies, such as antenatal education, diagnosis, and treatment of anaemia to prevent PPH. Their understanding of the condition as a life-threatening condition enabled the midwives to provide holistic and effective management that sometimes involved multidisciplinary team approach. However, inadequate resources and delay in seeking health care services militate against their efforts. The midwives also identified the need for continuing education and training as means of enhancing their standard of care.
Conclusion

These midwives in Nigerian rural health care setting engage in preventive practices and active management of PPH though not without barriers, such as inadequate resources. There is need for the midwives in rural areas to have cultural competence, be provided with adequate resources and participate in ongoing education to be more effective.