bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microstructure evolution and hard x-ray reflectance of ultrathin Ru/C multilayer mirrors with different layer thicknesses
Authors: Liu Yang , Huang Qiushi , Qi Runze , Xiao Liangxing , Zhang Zhong , Li Wenbin , Yi Shengzhen , Wang Zhanshan 
Published: January 2021

Abstract
Nanoscale Ru/C multilayers are essential reflective optics in the hard x-ray region of 7–20 keV. To understand the layer growth behavior and develop ultrathin Ru/C multilayer mirrors with periods smaller than 3.0 nm, multilayers with different periods of 6.2–1.5 nm were fabricated and studied. It is found that the average interface width started to increase obviously when the period became smaller than 2.5 nm while the surface roughness of different multilayers remained almost the same. The intrinsic stress of the multilayer gradually decreased with decreasing period and reached a very low value of −82 MPa at d = 2.3 nm. High reflectance of 54% and 65% (at E = 8.04 keV) were demonstrated for the multilayers with periods of 2.5 nm and 3.0 nm, respectively, whereas that for 1.9 nm period was significantly lower. To further analyze the layer microstructure, x-ray diffraction and transmission electron microscopy were used. The polycrystallized structure of Ru remained similar for the multilayers with period less than 2.5 nm, while a non-continuous layer growth and severe intermixing between Ru and C were observed for the multilayer with period of 1.9 nm. The increased intermixing between Ru and C was found to be the main reason for the larger interface width and lower reflectance of the multilayers with period smaller than 2.5 nm. It also indicated that the layer thickness threshold for a Ru/C multilayer growing with good layer quality is 1.0–1.2 nm.
Enhanced transmission performance based on ultrathin broadband circular hole array metasurface
Authors: Ruihua Li , Zhong Min 
Published: January 2021

Abstract
A high transmittance broadband metasurface with Si and STO layers is verified in 100–130 THz. This metasurface achieves a transmission peak (Amplitude 78.1%, Resonance frequency 112.2 THz) with a complete Si layer, or a broad transmission band (Average transmittance 82.3%, bandwidth 5.1 THz) with a Si layer defined by a circular hole array. The influence of structural parameters (period P and diameter D) on this transmission band is measured. In the first set of measurements, the average transmittance and bandwidth of this metasurface remain unchanged with P increasing. In the second set of measurements, however, the average transmittance is increased from 82.3% to 92.7%, and bandwidth is enhanced from 5.1 THz to 7.2 THz with D increasing. Finally, the sensitivity of the metasurface to temperature is measured. When temperature increases from 300 K to 360 K, average transmittance increases from 82.3% to 95.4%, and bandwidth enhances from 5.1 THz to 10.3 THz. When temperature reduces, the average transmittance and bandwidth decrease. This metasurface exploits the potential of temperature sensing.
Comparative Ultrastructural Analysis of Thalamocortical Innervation of the Primary Motor Cortex and Supplementary Motor Area in Control and MPTP-Treated Parkinsonian Monkeys
Authors: Villalba Rosa M , Behnke Joseph A , Pare Jean-Francois , Smith Yoland 
Published: January 2021

Abstract
The synaptic organization of thalamic inputs to motor cortices remains poorly understood in primates. Thus, we compared the regional and synaptic connections of vGluT2-positive thalamocortical glutamatergic terminals in the supplementary motor area (SMA) and the primary motor cortex (M1) between control and MPTP-treated parkinsonian monkeys. In controls, vGluT2-containing fibers and terminal-like profiles invaded layer II–III and Vb of M1 and SMA. A significant reduction of vGluT2 labeling was found in layer Vb, but not in layer II–III, of parkinsonian animals, suggesting a potential thalamic denervation of deep cortical layers in parkinsonism. There was a significant difference in the pattern of synaptic connectivity in layers II–III, but not in layer Vb, between M1 and SMA of control monkeys. However, this difference was abolished in parkinsonian animals. No major difference was found in the proportion of perforated versus macular post-synaptic densities at thalamocortical synapses between control and parkinsonian monkeys in both cortical regions, except for a slight increase in the prevalence of perforated axo-dendritic synapses in the SMA of parkinsonian monkeys. Our findings suggest that disruption of the thalamic innervation of M1 and SMA may underlie pathophysiological changes of the motor thalamocortical loop in the state of parkinsonism.
Acute pulmonary embolism in a patient with mild COVID-19 symptoms: a case report
Authors: Fiorini Norberto B , Garagoli Fernando , Bustamante Rosana C , Pizarro Rodolfo 
Published: January 2021

Abstract

Background
The venous thromboembolism (VTE) is a frequent condition, which may worsen the prognosis of hospitalized COVID-19 patients. Nevertheless, the incidence of this complication is unknown in patients with mild COVID-19 symptoms.

Case summary
A 26-year-old female nurse, who had been taking oral contraceptive pills (OCPs) treatment for the last 2 years, developed mild COVID-19 symptoms (rhinitis and anosmia). She underwent isolation at home and was subsequently followed up with telehealth visits. Fifteen days after her initial presentation, she developed acute onset sudden dyspnoea. On physical examination, she was found to be tachycardic with normal pulse oximetry. The initial risk score for VTE was moderate and laboratory results showed increased D-dimer level without other relevant findings. Computed tomography pulmonary angiography was performed, which confirmed low-risk subsegmental pulmonary embolism.

Discussion
Venous thromboembolism in patients who present with severe COVID-19 symptoms has already been described in the literature; its incidence is greater in patients hospitalized in intensive care units. Efforts to prevent VTE based on risk scores are widely recognized. However, the relationship in patients who present with mild COVID-19 symptoms and VTE is still unknown. Recently, experts on this field have introduced thromboprophylaxis guidelines including ambulatory patients based on the severity of COVID-19 symptoms and pro-thrombotic risk. Our patient showed no major risk for developing VTE; therefore, the VTE could be associated with SARS-CoV-2 infection or the eventual pro-thrombotic association with the concomitant use of OCPs.
Subannular repair for functional mitral regurgitation type IIIb in patients with ischaemic versus dilated cardiomyopathy
Authors: Pausch Jonas , Sequeira Gross Tatiana , Müller Lisa , von Stumm Maria , Kloth Benjamin , Reichenspurner Hermann , Girdauskas Evaldas 
Published: January 2021

Abstract

OBJECTIVES
Functional mitral regurgitation (FMR) is a sequel of left ventricular (LV) remodelling in heart failure patients. Relocation of both papillary muscles aims to specifically address mitral leaflet tethering to improve long-term durability of modern FMR repair. Nevertheless, the prognostic impact of the underlying cardiomyopathy on the outcome after FMR repair is unknown.

METHODS
We analysed 84 consecutive heart failure patients with severe FMR, LV ejection fraction &lt;40%, LV end-diastolic diameter ≥55 mm and tenting height &gt;10 mm, who underwent ring annuloplasty and simultaneous bilateral papillary muscles relocation between June 2016 and March 2019. One-year outcome of 54 patients with ischaemic cardiomyopathy (‘ICM group’) was prospectively compared to the remaining 30 patients with dilated cardiomyopathy (‘DCM group’).

RESULTS
One-year survival was similar in both groups (96% in the ‘ICM group’ vs 97% in the ‘DCM group’; P = 0.93). Furthermore, primary composite outcome (i.e. freedom from death or mitral regurgitation ≥ 2) at 1-year postoperatively was comparable between the study groups (94%in the ‘ICM group’ vs 87% in the ‘DCM group’; P = 0.10). LV end-diastolic diameter 1-year after surgery was significantly reduced, as compared to preoperative values, in the ‘DCM group’ (P = 0.018), but not in the ‘ICM group’ (P = 0.058). Improvement of New York Heart Association functional class and reduction of serum levels of N-terminal pro-B natriuretic peptide at 1 year was comparable in both study groups.

CONCLUSIONS
Standardized relocation of both papillary muscles to correct FMR resulted in very satisfactory in-hospital and 1-year outcomes, in both ICM and DCM. DCM patients showed similar improvement in heart failure symptoms and LV re-remodelling compared to ICM patients. Subannular repair is developing towards a valid therapeutic option in heart failure patients presenting with severe FMR.
Eif2b3 mutants recapitulate phenotypes of vanishing white matter disease and validate novel disease alleles in zebrafish
Authors: Lee Yu-Ri , Kim Se Hee , Ben-Mahmoud Afif , Kim Oc-Hee , Choi Tae-Ik , Lee Kang-Han , Ku Bonsu , Eum Juneyong , Kee Yun , Lee Sangkyu , Cha Jihoon , Won DongJu , Lee Seung-Tae , Choi Jong Rak , Lee Joon Soo , Kim Heung Dong , Kim Hyung-Goo , Bonkowsky Joshua L , Kang Hoon-Chul , Kim Cheol-Hee 
Published: January 2021

Abstract
Leukodystrophy with vanishing white matter (VWM), also called Childhood Ataxia with Central Nervous System Hypomyelination, is caused by mutations in the subunits of the eukaryotic translation initiation factor, EIF2B1, EIF2B2, EIF2B3, EIF2B4 or EIF2B5. However, little is known regarding the underlying pathogenetic mechanisms, and there is no curative treatment for VWM. In this study, we established the first EIF2B3 animal model for VWM disease in vertebrates by CRISPR mutagenesis of the highly conserved zebrafish ortholog eif2b3. Using CRISPR, we generated two mutant alleles in zebrafish eif2b3, 10- and 16-bp deletions, respectively. The eif2b3 mutants showed defects in myelin development and glial cell differentiation, and increased expression of genes in the induced stress response pathway. Interestingly, we also found ectopic angiogenesis and increased VEGF expression. Ectopic angiogenesis in the eif2b3 mutants was reduced by the administration of VEGF receptor inhibitor SU5416. Using the eif2b3 mutant zebrafish model together with in silico protein modeling analysis, we demonstrated the pathogenicity of 18 reported mutations in EIF2B3, as well as of a novel variant identified in a 19-month-old female patient: c.503 T &gt; C (p.Leu168Pro). In summary, our zebrafish mutant model of eif2b3 provides novel insights into VWM pathogenesis and offers rapid functional analysis of human EIF2B3 gene variants.
Collective Deterrence in the Shadow of Shifting Power
Authors: Phillips Julianne , Wolford Scott 
Published: January 2021

AbstractTwelve of twenty-six war-winning coalitions since 1815 have seen at least two members go to war against one another after victory. What separates durable and fragile war-winning coalitions? To answer this question, we analyze a game-theoretic model of shifting intra-coalition power and collective deterrence. We show that (1) shifting power within war-winning coalitions can undermine commitments to the postwar settlement, but (2) revisionist threats from a powerful defeated side can enhance the credibility of commitments within the winning coalition, securing peace when intra-coalition war would otherwise be inevitable. We also recover these patterns in empirical models of the outbreak of war between former coalition partners: shifting power within a coalition is associated with increased probabilities of intra-coalition war, but only when the defeated side is not too powerful. A common enemy can thus preserve peace between former partners who would otherwise go to war over the terms of shared victory.
Enhanced production of clavulanic acid by improving glycerol utilization using reporter-guided mutagenesis of an industrial Streptomyces clavuligerus strain
Authors: Shin Chang-Hun , Cho Hang Su , Won Hyung-Jin , Kwon Ho Jeong , Kim Chan-Wha , Yoon Yeo Joon 
Published: January 2021

Abstract
Clavulanic acid (CA) produced by Streptomyces clavuligerus is a clinically important β-lactamase inhibitor. It is known that glycerol utilization can significantly improve cell growth and CA production of S. clavuligerus. We found that the industrial CA-producing S. clavuligerus strain OR generated by random mutagenesis consumes less glycerol than the wild-type strain; we then developed a mutant strain in which the glycerol utilization operon is overexpressed, as compared to the parent OR strain, through iterative random mutagenesis and reporter-guided selection. The CA production of the resulting S. clavuligerus ORUN strain was increased by approximately 31.3% (5.21 ± 0.26 g/l) in a flask culture and 17.4% (6.11 ± 0.36 g/l) in a fermenter culture, as compared to that of the starting OR strain. These results confirmed the important role of glycerol utilization in CA production and demonstrated that reporter-guided mutant selection is an efficient method for further improvement of randomly mutagenized industrial strains.
A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging
Authors: Wazawa Tetsuichi , Noma Ryohei , Uto Shusaku , Sugiura Kazunori , Washio Takashi , Nagai Takeharu 
Published: January 2021

AbstractReversibly photoswitchable fluorescent proteins (RSFPs) are a class of fluorescent proteins whose fluorescence can be turned on and off by light irradiation. RSFPs have become essential tools for super-resolution (SR) imaging. Because most SR imaging techniques require high-power-density illumination, mitigating phototoxicity in cells due to intense light irradiation has been a challenge. Although we previously developed an RSFP named Kohinoor to achieve SR imaging with low phototoxicity, the photoproperties were insufficient to move a step further to explore the cellular dynamics by SR imaging. Here, we show an improved version of RSFP, Kohinoor2.0, which is suitable for SR imaging of cellular processes. Kohinoor2.0 shows a 2.6-fold higher fluorescence intensity, 2.5-fold faster chromophore maturation and 1.5-fold faster off-switching than Kohinoor. The analysis of the pH dependence of the visible absorption band revealed that Kohinoor2.0 and Kohinoor were in equilibria among multiple fluorescently bright and dark states, with the mutations introduced into Kohinoor2.0 bringing about a higher stabilization of the fluorescently bright states compared to Kohinoor. Using Kohinoor2.0 with our SR imaging technique, super-resolution polarization demodulation/on-state polarization angle narrowing, we conducted 4-h time-lapse SR imaging of an actin filament network in mammalian cells with a total acquisition time of 480 s without a noticeable indication of phototoxicity. Furthermore, we demonstrated the SR imaging of mitochondria dynamics at a time resolution of 0.5 s, in which the fusion and fission processes were clearly visualized. Thus, Kohinoor2.0 is shown to be an invaluable RSFP for the SR imaging of cellular dynamics.
A simple preparation method for CLEM using pre-embedding immunohistochemistry with a novel fluorescent probe and stable embedding resin
Authors: Kanemaru Takaaki , Kondo Teruyoshi , Nakamura Kei-ichiro , Morimoto Hiroyuki , Nishi Kentaro , Isobe Shin-ichiro 
Published: January 2021

Abstract
Correlative light and electron microscopy (CLEM) is an excellent approach for examining the cellular localization of biomolecules. Here, we developed a simple method for CLEM by combining pre-embedding immunohistochemistry with a novel fluorescent probe, namely Fluolid NS Orange, and an embedding resin called ʻDurcupan™ʼ. Specimens were embedded in Durcupan™ or LR White after immunolabeling and post-fixation using glutaraldehyde and osmium tetroxide. Next, ultrathin sections were prepared on a finder grid with navigation markers. The section of the specimen embedded in Durcupan™ was found to be more stable against electron beam irradiation than specimens embedded in LR White. A fluorescence light microscopy image and a transmission electron microscopy (TEM) image, at wide-field, and low magnification, were independently obtained with the same ultrathin section. Using the three corners between finder grid bars as landmarks, fluorescence light microscopy images were superimposed with wide-field, low-magnification TEM images to identify the region of interest, which was subsequently enlarged to ascertain cellular structures localized beneath fluorescent signals. However, the enlarged TEM images appeared blurred, and fluorescence signals had a hazy appearance. To resolve this, the enlarged TEM images were replaced by high-resolution TEM images focused directly on the region of interest, thereby facilitating the collection of high-resolution CLEM images. The simple sample processing method for CLEM using osmium-resistant Fluolid NS Orange and electron beam damage-resistant Durcupan™ allowed the determination of the precise localization of fluorescence signals at subcellular levels.
A project review under the focus of ‘complexities’ on the example of exploreAT!
Authors: Dorn Amelie , Wandl-Vogt Eveline , Palfinger Thomas , Therón Roberto , Way Andy , Abgaz Yalemisew , Benito Alejandro 
Published: January 2021

Abstract
This article provides insights into dealing with complexities in the Digital Humanities project exploreAT!. By exploring a non-standard language collection for cultural insights, a three-fold approach is presented looking into concrete realizations and solutions of tackling challenges in terms of Open Innovation infrastructure, technology and the topic of choice, food. Methods and processes applied and developed in the project are aimed to serve as examples for future projects with similar data sets.
Polarization of hot Jupiter systems: a likely detection of stellar activity and a possible detection of planetary polarization
Authors: Bailey Jeremy , Bott Kimberly , Cotton Daniel V , Kedziora-Chudczer Lucyna , Zhao Jinglin , Evensberget Dag , Marshall Jonathan P , Wright Duncan , Lucas P W 
Published: January 2021

ABSTRACT
We present high-precision linear polarization observations of four bright hot Jupiter systems (τ Boo, HD 179949, HD 189733, and 51 Peg) and use the data to search for polarized reflected light from the planets. The data for 51 Peg are consistent with a reflected light polarization signal at about the level expected with 2.8σ significance and a false alarm probability of 1.9 per cent. More data will be needed to confirm a detection of reflected light in this system. HD 189733 shows highly variable polarization that appears to be most likely the result of magnetic activity of the host star. This masks any polarization due to reflected light, but a polarization signal at the expected level of ∼20 ppm cannot be ruled out. τ Boo and HD 179949 show no evidence for polarization due to reflected light. The results are consistent with the idea that many hot Jupiters have low geometric albedos. Conclusive detection of polarized reflected light from hot Jupiters is likely to require further improvements in instrument sensitivity.
The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba
Authors: Žárský Vojtěch , Klimeš Vladimír , Pačes Jan , Vlček Čestmír , Hradilová Miluše , Beneš Vladimír , Nývltová Eva , Hrdý Ivan , Pyrih Jan , Mach Jan , Barlow Lael , Stairs Courtney W , Eme Laura , Hall Neil , Eliáš Marek , Dacks Joel B , Roger Andrew , Tachezy Jan 
Published: January 2021

Abstract
The transition of free-living organisms to parasitic organisms is a mysterious process that occurs in all major eukaryotic lineages. Parasites display seemingly unique features associated with their pathogenicity; however, it is important to distinguish ancestral preconditions to parasitism from truly new parasite-specific functions. Here, we sequenced the genome and transcriptome of anaerobic free-living Mastigamoeba balamuthi and performed phylogenomic analysis of four related members of the Archamoebae, including Entamoeba histolytica, an important intestinal pathogen of humans. We aimed to trace gene histories throughout the adaptation of the aerobic ancestor of Archamoebae to anaerobiosis and throughout the transition from a free-living to a parasitic lifestyle. These events were associated with massive gene losses that, in parasitic lineages, resulted in a reduction in structural features, complete losses of some metabolic pathways, and a reduction in metabolic complexity. By reconstructing the features of the common ancestor of Archamoebae, we estimated preconditions for the evolution of parasitism in this lineage. The ancestor could apparently form chitinous cysts, possessed proteolytic enzyme machinery, compartmentalized the sulfate activation pathway in mitochondrion-related organelles, and possessed the components for anaerobic energy metabolism. After the split of Entamoebidae, this lineage gained genes encoding surface membrane proteins that are involved in host–parasite interactions. In contrast, gene gains identified in the M. balamuthi lineage were predominantly associated with polysaccharide catabolic processes. A phylogenetic analysis of acquired genes suggested an essential role of lateral gene transfer in parasite evolution (Entamoeba) and in adaptation to anaerobic aquatic sediments (Mastigamoeba).
Gorgocephalidae (Digenea: Lepocreadioidea) in the Indo-West Pacific: new species, life-cycle data and perspectives on species delineation over geographic range
Authors: Huston Daniel C , Cutmore Scott C , Miller Terrence L , Sasal Pierre , Smit Nico J , Cribb Thomas H 
Published: January 2021

AbstractThe digenetic trematode family Gorgocephalidae comprises just a few species, and the literature devoted to the lineage consists of only a handful of reports. With one exception, all reports have been based on material collected in the Indo-West Pacific, an expansive marine ecoregion stretching from the east coast of Africa to Easter Island, Hawaii and French Polynesia. We collected adult and intramolluscan gorgocephalids from kyphosid fishes and littorinid gastropods from several Australian localities, and from South Africa and French Polynesia. Specimens of Gorgocephalus kyphosi and G. yaaji were collected from, or near, their type-localities, providing new morphological and molecular (COI, ITS2 and 28S) data needed for a revised understanding of species boundaries in the family. Two new species are recognized: Gorgocephalus euryaleae sp. nov. and Gorgocephalus graboides sp. nov. New definitive host records are provided for described species and three new intermediate hosts are identified. These new records are all associated with Kyphosus fishes and littorinid gastropods, reaffirming the restriction of gorgocephalids to these hosts. Most significantly, we provide evidence that G. yaaji is distributed from South Africa to French Polynesia, spanning the breadth of the Indo-West Pacific. Our findings have significant relevance regarding digenean species delineation over geographic range.