bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gain-of-Function NH787 Ethylmethanesulfonate Mutant of Nagina22 Rice Variety Confers Augmented PUE
Authors: Yugandhar Poli , Veronica Nallamothu , Ai Hao , Goud Muddapuram Deeksha , Wang Xiaowen , Subrahmanyam Desiraju , Satendra Mangrauthia , Ajay Jain 
Published: February 2021

Abstract
Rice (Oryza sativa L.), a major dietary source, is often cultivated in soils poor in available inorganic orthophosphate (Pi), which is a pivotal nutrient for growth and development. Poor soils are amended by phosphorus (P) fertilizer, which is derived from the non-renewable rock phosphate reserves. Therefore, there is a need for developing rice varieties with high productivity under low P conditions. At the ICAR-IIRR, ethyl methanesulfonate (EMS) mutagenized rice genotype Nagina22 (N22) were screened for high grain yield in Pi-deprived soil, which led to the identification of ~10 gain-of-function mutants including NH787. Here, detailed comparative morphophysiological, biochemical, and molecular analyses of N22 and NH787 were carried out in hydroponics and potting soil under different Pi regimes. Under Pi-deprived conditions, compared with N22, NH787 exhibited higher root and vegetative biomass, the number of tillers, and grain yield. The augmented agronomic traits of NH787 were corroborated with significantly higher photosynthetic rate, pollen fertility, stigma receptivity, and the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Further, several genes involved in the maintenance of Pi homeostasis (GPH) were differentially regulated. The study thus revealed a wide-spectrum influence of the mutation in NH787 that contributed towards its higher Pi use efficiency.
Genetic Association of ERAP1 and ERAP2 With Eclampsia and Preeclampsia in Northeastern Brazilian Women
Authors: FERREIRA LEONARDO CAPISTRANO , GOMES CARLOS EDUARDO MAIA , DUGGAL PRIYA , HOLANDA INGRID DE PAULA , DE LIMA AMANDA SAMARA , NASCIMENTO PAULO RICARDO PORFÍRIO DO , JERONIMO SELMA MARIA BEZERRA 
Published: February 2021

Abstract
The clinical spectrum of hypertensive disorders of pregnancy (HDP) is determined by the interplay between environmental and genetic factors, most of which remains unknown. ERAP1, ERAP2 and LNPEP genes code for multifunctional aminopeptidases involved with antigen processing and degradation of small peptides such as angiotensin II (Ang II), vasopressin and oxytocin. We aimed to test for associations between genetic variants in aminopeptidases and HDP. A total of 1282 pregnant women (normotensive controls, n=693; preeclampsia, n=342; chronic hypertension with superimposed preeclampsia, n=61; eclampsia, n=74; and HELLP syndrome, n=112) were genotyped for variants in LNPEP (rs27300, rs38034, rs2303138), ERAP1 (rs27044, rs30187) and ERAP2 (rs2549796 rs2927609 rs11135484). We also evaluated the effect of ERAP1 rs30187 on plasma Ang II levels in an additional cohort of 65 pregnant women. The genotype C/C, in ERAP1 rs30187 variant (c.1583T>C, p.Lys528Arg), was associated with increased risk of eclampsia (OR=1.85, p=0.019) whereas ERAP2 haplotype rs2549796(C)-rs2927609(C)-rs11135484(G) was associated with preeclampsia (OR=1.96, corrected p-value=0.01). Ang II plasma levels did not differ across rs30187 genotypic groups (p=0.895). In conclusion, ERAP1 gene is associated with eclampsia whereas ERAP2 is associated with preeclampsia, although the mechanism by which genetic variants in ERAPs influence the risk of preeclampsia and eclampsia remain to be elucidated.
Occurrence and Potentially Zoonotic Genotypes of Enterocytozoon Bieneusi in Wild Rhesus Macaques (Macaca Mulatta) Living in Nanwan Monkey Island, Hainan, China: A Public Health Concern
Authors: Zhao Wei , Zhou Huan-Huan , Ren Guang-Xu , Qiang Yu , Huang Hui-Cong , Lu Gang , Tan Feng 
Published: February 2021

Abstract
Background

Enterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques (Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential.
Methods

We collected 173 fecal specimens from wild M. mulatta living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi.
Results

Of the 173 specimens from wild M. mulatta, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 (n = 20), D (n = 2), Type IV (n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX (n = 1). From the phylogenetic analysis, the six genotypes identified here were all categorized into zoonotic group 1.
Conclusion

Based on the results taht the novel genotype falling under zoonotic group 1 and all the known genotypes found in humans, we conclude that the wild M. mulatta infected with E. bieneusi have a public health significance.
Effect of Low-Density Lipoprotein Cholesterol Goal Achievement on Vascular Physiology Evaluated by Quantitative Flow Ratio in Patients Underwent Percutaneous Coronary Intervention
Authors: Chen Long , Zhong Jiaxin , Ye Zhen , Chen Qin , Ye Mingfang , Yan Yuanming , Luo Yukun 
Published: February 2021

Abstract
Purpose

The change in coronary physiology from lipid-lowing therapy(LLT)lacks an appropriate examination method. Quantitative flow ratio (QFR) is a novel angiography-based method allowing fast assessment of coronary physiology. This study sought to determine the impact of the low-density lipoprotein cholesterol (LDL-C) goal achievement on coronary physiology through QFR.
Methods

Patients who underwent percutaneous coronary intervention (PCI) and one-year angiographic follow-up were screened and computed by QFR analysis. According to the LDL-C level at one-year follow-up, patients were divided into two groups:1) goal-achievement group (LDL-C < 1.8 mmol/l or reduction of ≥ 50%, n = 146, lesion = 165); 2) non-achievement group (n = 286, lesion = 331). All QFR data and major adverse cardiovascular and cerebrovascular events (MACCEs) at one year were compared between groups.
Results

No differences between the groups were found in quantitative coronary angiography (QCA) data and QFR at post-PCI. At one-year follow-up, lower percentage diameter stenosis (DS %) and percentage area stenosis (AS %) were recorded in goal-achievement group (27.89 ± 10.16 vs. 30.93 ± 12.03, p = 0.010, 36.57 ± 16.12 vs. 41.68 ± 17.39, p = 0.003, respectively). Meanwhile, a higher QFR was found in goal-achieved patients (0.96 ± 0.05 vs. 0.94 ± 0.09, p = 0.005). Furthermore, goal-achievement group had a lower incidence of physiological restenosis and MACCEs (2.1% vs. 8.4%, p = 0.018, 5.4 % vs. 12.6 %, p = 0.021, respectively).
Conclusions

Evaluated by QFR, patients who achieved the LDL-C goal seem to have a better coronary physiological benefit. Meanwhile, this group of patients is presented a better clinical outcome.
“Double X” Fixation for Rare and Particular Pediatric Supracondylar Humerus Fractures
Authors: Burnei Gheorghe , Răducan Ionut Daniel , Enache Florin-Daniel , Dărăban Adriana Maria , Avram Cecilia Roberta , Burnei Cristian 
Published: February 2021

Abstract
Background Context. The presence of special, rare and various forms which we can encounter when treating supracondylar humeral fractures (SHF) in children, call into question what therapeutic methods can be used to increase the effectiveness of the treatment applied. The aim of this paper is to present the results obtained by using double cross-fixation, "in double X", by closed reduction and percutaneous pinning (CRPP) or open reduction and internal fixation (ORIF) in treating rare and particular forms. Comments and opinions on "double X" method and other cross or side configurations are presented. Patient Sample. All patients with rare and particular forms presented in this article, were operated during 2001–2020 in state and private hospitals. Fixation in "double X" was used either by CRPP and ORIF. The epitrochlear approach (EA) was performed in cases where stability by CRPP could not be ensured or when there were clear signs of ulnar nerve damage. The minimal medial-approach (mMA) highlights the epitrochlea and the fracture of the medial pylon; it has the role of anatomically reducing the medial pylon, thinner and very unstable in rare and particular forms. During 1982–2020 we consulted, treated and evaluated patients with SHF to whom all known treatment methods were applied: orthopedic reduction and immobilization in plaster cast, CRPP, ORIF, minimal-open reduction and internal fixation (mORIF) by mMA and external fixator. Internal fixation was done by the techniques of Judet, San Antonio, San Diego, Dorgan, in “X” and “double X”. In 2001, I introduced "double X" fixation to better stabilize anatomical reductions that showed signs of instability during intraoperative verification. All indications given in the study protocol have been made in accordance with the regulations mentioned in the experimental program. Results. All SHFs operated by CRPP and ORIF by “double X” were cured and satisfactory and good results were obtained in the neglected types operated between 14 and 60 days and good and excellent results were obtained in the rare forms. Conclusions. “Double X” fixation gives the best stabilization and postoperatively there is no need for immobilization in a plaster cast. Recovery may begin the day after surgery.
Phosphorylation of β1-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy
Authors: Saito Nobumichi , Kondo Masumi , Ono Moe , Kaneyama Noriko , Abe Makiko , Sawada Kaichiro , Moriya Hitomi , Kimura Moritsugu , Toyoda Masao , Fukagawa Masafumi 
Published: February 2021

Abstract
The induction of a high blood pressure due to diabetic nephropathy depends on the increase in renin secretion from juxtaglomerular cells, but many aspects of how juxtaglomerular cells sense blood pressure changes in the afferent arteriole and consequently react remain unclear. In this study, we detected the juxtaglomerular cell-specific phosphorylation of the threonine-788/789 site of β1-integrin, and its expression was negatively correlated with renin production. This relationship was also observed in a culture system of a juxtaglomerular cell line, suggesting that β1-integrin is deeply involved in the regulation of renin production. The knockdown of β1-integrin in the culture system increased renin production, but the degree of the increase was comparable to the increase in renin production by knockdown of connexin-40, which is considered to be an important molecule that plays a role in the pressure sensing mechanism of juxtaglomerular cells. This suggests that the mechanism underlying the regulation of renin production by β1-integrin in juxtaglomerular cells may contribute to the pressure-sensing function of juxtaglomerular cells themselves. Threonine-788/789 phosphorylation of β1-integrin may be involved in the regulation of this pressoreceptor function. (176 words)
The Influence of Widow Size on Remote Sensing-Based Prediction of Forest Structural Variables
Authors: ÖZKAN Ulaş Yunus , Demirel Tufan 
Published: February 2021

Abstract
Background: Determining the appropriate window size is a critical step for estimating stand structural variables based on remote sensing data. Because the value of the reference laser and image metrics that affect the quality of the prediction model depends on window size. However, suitable window sizes are usually determined by trial and error. There are a limited number of published studies evaluating appropriate window sizes for different remote sensing data. This research investigated the effect of window size on predicting forest structural variables using airborne LiDAR data, digital aerial image and WorldView-3 satellite image.Results: In the WorldView-3 and digital aerial image, significant differences were observed in the prediction accuracies of the structural variables according to different window sizes. For the estimation based on WorldView-3 in black pine stands, the optimal window sizes for stem number (N), volume (V), basal area (BA) and mean height (H) were determined as 1000 m2, 100 m2, 100 m2 and 600 m2, respectively. In oak stands, the R2 values of each moving window size were almost identical for N and BA. The optimal window size was 400 m2 for V and 600 m2 for H. For the estimation based on aerial image in black pine stands, the 800 m2 window size is optimal for N and H, the 600 m2 window size is optimal for V and the 1000 m2 window size is optimal for BA. In the oak stands, the optimal window sizes for N, V, BA and H were determined as 1000 m2, 100 m2, 100 m2 and 600 m2, respectively. The optimal window sizes may need to be scaled up or down to match the stand canopy components. In the LiDAR data, the R2 values of each window size were almost identical for all variables of the black pine and the oak stands.Conclusion: This study illustrated that the window size has an effect on the prediction accuracy in estimating forest structural variables based on remote sensing data. Moreover, the results showed that the optimal window size for forest structural variables varies according to remote sensing data and tree species composition.
A Free-standing Nanoporous NiCoFeMoMn High Entropy Alloy as An Efficient Electrocatalyst Fast Driving Water Splittling
Authors: Liu Hao , Qin Hongye , Kang Jianli , Ma Liying , Chen Guoxin , Huang Qin , Zhang Zhijia , Liu Enzuo , Lu Huanming , Zhao Naiqin 
Published: February 2021

Abstract
The development of low-cost non-noble metal-based electrocatalysts that can work stably at high current densities for the application of Hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) in electrolyzed water is paramount crucial. Herein, we report a free-standing nanoporous high entropy alloy foil as dual-functional electrocatalyst via a combination of one-step dealloying and polarization, which exhibits excellent electrocatalytic activity in alkaline electrolyte with an extremely small overpotential of 150 mV at 1000 mA cm-2 for HER and a low Tafel slope of 29 mV dec-1 in 1 M KOH solution; At the same current density, the overpotential of OER is only 350 mV. The alkaline electrolyzer using it as both anode and cathode only need a cell voltage of 1.47 V to output a stable current density of 10 mA cm-2, enabling it as an efficient bifunctional electrocatalyst for alkaline overall water splitting, and remarkably better stability for more than 375 hours of continuous hydrogen production while providing substantial material cost savings relative to platinum. DFT calculations indicate that the ultrahigh HER activity of catalyst is originated from the synergetic eﬀect of optimized hydrogen adsorption in the segregation area and enhanced H2O adsorption in the un-segregation area produced by spinodal decomposition.
Hurdles to Developing and Scaling Remote Patients’ Health Management Tools &amp; Systems: A Scoping Review
Authors: Ryobeza Barimwotubiri , Grobbelaar Sara , Botha Adele 
Published: February 2021

Abstract
Background: Despite all the excitement and hype generated regarding the expected transformative impact of digital technology on the healthcare industry, traditional healthcare systems around world have largely remained unchanged and resultant improvements in developed countries are slower than anticipated. One area which was expected to significantly improve the quality of and access to primary healthcare services in particular, is remote patient monitoring and management. Based on a combination of rapid advances in body sensors and information and communication technologies (ICT), it was hoped that remote patient management tools and systems (RPMTSs) would significantly reduce the care burden on traditional healthcare systems as well as health related costs. However, the uptake or adoption of above systems has been extremely slow and their roll out has not yet properly taken off especially in developing countries where they ought to have made the greatest positive impact. Aim: The aim of the study was to assess whether or not recent, relevant literature would support the development of in-community, design, deployment and implementation framework based on three factors thought to be important drivers and levers of RPMTS’s adoption and scalability. Methods: A rapid, scoping review conducted on relevant articles obtained from PubMed, MEDLINE, PMC and Cochrance databases and grey literature on google and published between 2012 and May 2020, by combining a number of relevant search terms and phrases. Results: Most RPMTSs are targeted at and focused on a single disease, do not extensively involve patients and clinicians in their early planning and design phases, are not designed to best serve a specific catchment area and are mainly directed at post-hospital, diseases management settings. This may be leading to a situation where patients, potential patients and clinicians simply do not make use of these tools, leading to low adoption and scalability thereof. Conclusion: The development of a user-centered, context dependent, customizable design and deployment framework could potentially increase the adoption and scalability of RPMTSs, if such framework addressed a combination of diseases, prevalent in a given specific catchment area, especially in developing countries with limited financial resources.
Post-Annealing Effect on Optical and Electronic Properties of Thermally Evaporated MoOX Thin Films as Hole-Selective Contacts for p-Si Solar Cells
Authors: Jiang Yuanwei , Cao Shuangying , Lu Linfeng , Du Guanlin , Lin Yinyue , Wang Jilei , Yang Liyou , Zhu Wenqing , Li Dongdong 
Published: February 2021

Abstract
Owing to its large work function, MoOX has been widely used for hole-selective contact in both thin film and crystalline silicon solar cells. In this work, thermally evaporated MoOX films are employed on the rear sides of p-type crystalline silicon (p-Si) solar cells, where the optical and electronic properties of the MoOX films as well as the corresponding device performances are investigated as a function of post-annealing treatment. The MoOX film annealed at 100oC shows the highest work function and proves the best hole selectivity based on the results of energy band simulation and contact resistivity measurements. The full rear p-Si/MoOX/Ag contacted solar cells demonstrate the best performance with an efficiency of 19.19%, which is the result of the combined influence of MoOX’s hole selectivity and passivation ability.
Thrombospondin-2 Holds Prognostic Values and is Associated with the Metastasis and Mismatch Repair Process in Gastric Cancer
Authors: Chu Xiao-dong , Lin Zheng-bin , Huang Ting , Ding Hui , Zhang Yi-ran , Zhao Zhan , Huangfu Shu-chen , Qiu Sheng-hui , Guo Yan-guan , Chu Xiao-li , Pan Jing-hua , Pan Yun-long 
Published: February 2021

Abstract
Background: The study aims to investigate the expression level of Thrombospondin 2 (TSP2) in Gastric Cancer (GC) and determine the relationship between TSP2 and clinical characteristics and prognosis.Methods: The online database Gene Expression Profile Interactive Analysis (GEPIA) was used to analyze the mRNA expression level of TSP2 in GC. The Kaplan-Meier plotter prognostic analysis tool was used to evaluate the influence of TSP2 expression on clinical prognosis in GC patients. The expression level of TSP2 was analyzed in paraffin-embedded GC samples and adjacent normal tissues by immunohistochemistry. The relationship between clinicopathological characteristics and prognosis of GC patients. Next, the relationship between clinicopathological characteristics and prognosis of GC patients was assessed. Transwell experiment was used to evaluate the effect of TSP2 on the invasion and migration of HGC27 and AGS cells.Results: Compared with normal tissues, the expression of TSP2 mRNA in GC was significantly up-regulated, and it was closely related to the clinical stage of GC. The high expression of TSP2 significantly affected the OS, FP and PPS of patients with GC. Among them, the expression level of TSP2 did not affect the prognosis of patients with GC in N0 subgroup, but significantly affected the prognosis of patients with GC in N (1+2+3)subgroup. The protein expression level of TSP2 in GC tissue was significantly higher than in normal tissues (P<0.01). The overall survival (OS) rate of patients with high TSP2 expression was lower than the low TSP2 expression group (P=0.013). Knockdown of TSP2 can significantly inhibit the growth of GC cells. Proliferation, migration, invasion ability, and TSP2 expression level significantly correlate with mismatch repair genes such as PMS2, MSH6, MSH2, and MLH1 (P<0.05).Conclusion: The expression of TSP2 in GC is significantly increased, closely related to the metastasis and mismatch repair process of GC patients and affected GC patients' prognosis. It is a potential marker and treatment target for the prognosis of GC patients.
Curcubinoyl-conjugated flavonoids from methyl jasmonate-treated wild ginseng adventitious root cultures
Authors: Liu Qing , Kim Seon Beom , Jo Yang Hee , Ahn Jong Hoon , Turk Ayman , Kim Da Eun , Chang Bo Yoon , Kim Sung Yeon , Jeong Cheol-Seung , Hwang Bang Yeon , Park So-Young , Lee Mi Kyeong 
Published: February 2021

Abstract
Wild ginseng (Panax ginseng) adventitious root cultures were prepared by elicitation using methyl jasmonate and investigated further to find new secondary metabolites. Chromatographic fractionation of wild ginseng adventitious root cultures led to the isolation of eleven compounds. The chemical structures of isolated compounds were identified as four known flavanone derivatives (1–4), one new curcubinoyl derivative, jasmogin A (5) and six new curcubinoyl-flavanone conjugates, jasmoflagins A-F (6–11) by extensive spectroscopic analysis. Newly isolated curcubinoyl derivatives showed inhibitory activity against lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages. Curcubinoyl moiety is structurally similar with methyl jasmonate, which was used as an elicitor. Therefore, our present study suggested that elicitor might increase the content of active constituents and also contribute to the production of new metabolites by participation as precursors in biosynthesis. Taken together, plant cell cultures are useful not only for efficient production of active compounds but also development of new secondary metabolites.