bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune-Related DNA Methylation Data-Based Molecular Classification Associated with the Prognosis of Patients with Hepatocellular Carcinoma
Authors: Wang Xiong-Wen , Yan Qian , Ye Bao-Qian , Wang Bo-Qing , Zheng Wen-Jiang 
Published: May 2021

Abstract
Background: The combination of epigenetic drugs and immunotherapy should be able to develop an optimal treatment plan for hepatocellular carcinoma (HCC), yet its mechanism is still in the preliminary exploration stage. The purpose of this study is to analyze the DNA methylation and gene expression profiles of immune-related CpG sites to identify the molecular subtypes and CpG sites related to the prognosis of HCC. Methods: In this study, the DNA methylation and gene expression datasets were downloaded from The Cancer Genome Atlas database, together with immune-related genes downloaded from the immunology database and analysis portal database to explore the prognostic molecular subtypes of HCC. Univariate and multivariate survival analysis was used for selecting the significant methylation sites, and the consensus clustering was performed to find the best molecular subtype associated with the survival of HCC. Next, we used the least absolute shrinkage and selection operator (LASSO) algorithm to construct a prognostic-related model and performed internal verification. Finally, we explored the levels of 16 immune-related genes expression correlate with the infiltration levels of immune cells in HCC. Results: By performing consistent clustering analysis on 830 immune-related CpG sites in 231 samples of a training set, we identified seven subgroups with significant differences in overall survival. Finally, 16 classifiers of immune-related CpG sites were constructed and used in the testing set to verify the prognosis of DNA methylation subgroups, and the results were consistent with the training set. Using the TIMER database, we analyzed 16 immune-related CpG sites expression with the abundance of six types of immune infiltrating cells and found that most are positively correlated with the level of infiltration of multiple immune cells in HCC. Conclusions: This study screened potential immune-related prognostic methylation sites and established a new prognosis model of HCC based on DNA methylation molecular subtype, which may help in the early diagnosis of HCC and developing more effective personalized treatments.
Spectrum of Germline RET Variants Identified by Targeted Sequencing and Associated Multiple Endocrine Neoplasia Type 2 Susceptibility in China
Authors: Qi Xiaoping , Zhao Jianqiang , Fang Xudong , Lian Bijun , Li Feng , Wang Huihong , Cao Zhilie , Zheng Weihui , Cao Juan , Chen Yu 
Published: May 2021

Abstract
Background: Germline RET mutations and variants are involved in development of multiple endocrine neoplasia type 2 (MEN2). The present study investigated a spectrum of RET variants, analyzed genotype-phenotype relationships, and evaluated their effect on the MEN2 phenotype in Han Chinese patients. Methods: Targeted sequencing detected germline RET variants in 697 individuals, including 245 MEN2, 120 sporadic medullary thyroid cancer (MTC), and 15 pheochromocytoma (PHEO) patients and their 493 relatives. In silico analyses and classifications following ACMG-2015 were performed. Demographic, clinical variant types, and endocrine neoplasia molecular diagnosis records were also analyzed. Results: Nineteen different RET mutations (18 point and 1 del/ins mutations) in 214 patients with MEN2A (97.7%) or MEN2B (2.3%) were found, of which exon 11/10 mutations accounted for 79% (169/214). Nineteen compound mutations were found in 31 patients with MEN2A. Twenty-three variants (18 single and 5 double base substitution/compound variants) non-classification were also found. Of these, 17 ( 3 of pathogenic, 10 of uncertain significance, 2 of likely benign and 2 as benign) were found in 31 patients with MTC/PHEO. The remaining 6 variants (4 of uncertain significance and 2 of likely benign) found in 8 carriers had no evidence of MEN2. The entire cohort showed MEN2A-related PHEO, all occurring in exons 11/10, particularly at C634. Kaplan-Meier curves showed age-dependent penetration rates of MTC and PHEO, and occurrence rates of PHEO in patients with exon 11 mutations were all higher than those within exon 10; these bilateral PHEO were always associated with exon 11 mutations (all P < 0.05). While patient offspring had PHEO, parents with MEN2A had none, the frequency was approximately 10%. Interestingly, at least 6.8% of families were adoptive. Also, 3 non-hotspot RET variants (R114H, T278N, and D489N) appeared with high frequency. Conversely, polymorphism S836S was absent. Conclusions: These data are largely consistent with current evidence-based recommendations in the clinical practice guidelines. Diversity of RET variants or carriers may involve a different natural disease course. Further large-scale targeted sequencing studies will serve as an accurate and cost-effective approach to investigating MEN2 genotype-phenotype correlations for discovery of rare or unknown variants of RET.
Registered Cooperatives and Local Government Unit in the Province of Nueva Ecija Philippines: Status, Participation and the Way Forward
Authors: Gabriel Arneil , Sarmiento Raymund , Viernez Maridel 
Published: May 2021

Abstract
This study described the status of registered cooperatives in Nueva Ecija using descriptive–correlational research and a combination of quantitative and qualitative methods. This study found out that majority of the respondent cooperatives are operating for more than 25 years; with more than Million authorized capital; have annual net surplus of P1.2 Million or more; are multi–purpose; and with more than 100 members. The Local Government Units(LGU) are active in teaching the members of the cooperatives to abide rules and guidelines and in supporting the role of cooperatives in the province. However, they should provide aid to cooperatives to efficiently deal with their problems. Additionally, the LGU is informing the cooperatives about their rights and responsibilities. They are not suppressing cooperative members’ opinions and they accept queries coming from the cooperative members. Decision–making responsibilities regarding cooperative matters with the aid of the government were shared by the officers of Cooperatives and LGU. The LGU trust the cooperatives by giving full degree of power of all the benefits that are provided to them by the government. Likewise, cooperatives have no systematic source of authority and influence over the LGU. Despite of the difference in responses of the respondents in non–participation and citizens power, they are similar in tokenism as level of participation. Cooperatives that are new in existence, with low capitalization and with less number of members needed high degree of participation with their members and high degree of partnership and support with the Local Government Units (LGUs). The respondents recognized the help of the LGU in their promotion and in providing trainings but intensified aid and empowerment of the cooperatives are requested by the members and officers to the LGUs. Lastly, the respondent- cooperatives requested that establishment of cooperative office, strengthened tie-up with the LGU, information dissemination on cooperativism, increase aid and funding and partnership with other entities are means to heightened cooperatives participation in development.
Stress and associated factors among frontline healthcare workers in the COVID-19 epicenter of Da Nang city, Vietnam
Authors: Nguyen Nhan , Le Duong Dinh , Colebunders Robert , Fodjo Joseph Nelson Siewe , Tran Trung Dinh , Vo Thang Van 
Published: May 2021

Abstract

Frontline healthcare workers (HCWs) involved in the COVID-19 response stand a higher risk of experiencing psychosocial distress amidst the pandemic. Between July and September 2020, a second wave of the COVID-19 pandemic appeared in Vietnam with Da Nang city being the epicenter. During the outbreak, HCWs were quarantined within the health facilities in a bid to limit the spread of the COVID-19 to their respective communities. Using the stress component of the 21-item Depression, Anxiety and Stress Scale (DASS-21), we assessed the level of stress among HCWs in Da Nang city. Between 30th August and 15th September 2020, 746 frontline HCWs were recruited to fill an online structured-questionnaire. Overall, 44.6% of participants experienced increased stress and 18.9% severe or extremely severe stress. In multivariable analysis, increased stress was associated with longer working hours (OR=1.012; 95% CI: 1.004–1.019), working in health facilities providing COVID-19 treatment (OR=1.58, 95% CI: 1.04-2.39), having direct contact with patients or their bio-samples (physicians, nurses and laboratory workers; OR=1.42, 95% CI: 1.02-1.99), low confidence in the available personal protective equipment (OR=0.846; 95% CI: 0.744–0.962), and low knowledge on COVID-19 prevention and treatment (OR=0.853; 95% CI: 0.739-0.986). In conclusion, many frontline HCWs experienced an increased stress during the COVID-19 outbreak in Da Nang city. Reducing working time, providing essential personal protective equipment, enhancing of the knowledge on COVID-19 will help to reduce this stress. Moreover, extra support is needed for HCWs who are directly exposed to COVID-19 patients.
Epidemiological associations with genomic variation in SARS-CoV-2
Authors: Rahnavard Ali , Dawson Tyson , Clement Rebecca , Stearrett Nathaniel , Pérez-Losada Marcos , Crandall Keith A. 
Published: May 2021

Abstract
The 2019 novel coronavirus (SARS-CoV-2) is the etiological agent of the COVID-19 pandemic and evolves to evade both host immune systems and intervention strategies. To diminish the short-term and long-term impacts of coronavirus (CoV), we investigated CoV differences at the nucleotide and protein level and CoV genomic variation associated with epidemiological variation and geography. We divided the CoV genome into 29 constituent regions for this analysis. Our results highlight the variation of CoV variants of lineage and show that nonstructural protein 3 (nsp3) and Spike protein (S) have the highest variation and greatest correlation with the viral whole-genome variation, which makes these two proteins potential targets for treatments. S protein variation is highly correlated with nsp3, nsp6, and 3'−to−5' exonuclease. Country of origin and time since the start of the pandemic were the most influential metadata in these differences. Host sex and age are the lowest in terms of explaining the virus genome variation. We quantified variation explained by regions of the CoV genome across different CoV viruses including, SARS-CoV-2, Middle East respiratory syndrome coronavirus (MERS-CoV), other severe acute respiratory syndrome coronavirus SARS-CoV (SARS-related), and bat-derived severe acute respiratory syndrome (SARS)-like coronaviruses (Bat-SL-CoV). We found that Spike protein and nsp3 explain most of the variation among these viruses; they are also among the genomic regions with the highest number of sites under natural selection. Our results provide a direction to prioritize genes associated with outcome predictors, including health, therapeutic, and vaccine outcomes, and to inform improved DNA tests for predicting disease status.
Transfusion practices for patients with sickle cell disease at major academic medical centers participating in the Atlanta Sickle Cell Consortium
Authors: Winkler A.M. , Josephson C.D. 
Published: May 2021

Abstract
The Atlanta Sickle Cell Consortium represents more than 2600 pediatric and adult patients with sickle cell disease (SCD) in the metropolitan Atlanta, Georgia, area receiving care at four major locations, each providing comprehensive care 24 hours a day, 7 days a week. Both transfusion services that support these sites use two levels of prospective phenotype matching to decrease the rates of alloimmunization. Although exact rates are unknown and are currently under investigation, alloimmunization occurs infrequently with the exception of chronically transfused SCD patients, who represent the minority of active SCD patients. With increasing availability, red blood cell genotyping will be used in the near future both for determination of predicted patient phenotypes and for provision of genotypically matched donor units.
Immunohematology

2012;28:24–6.
The ISBT 700 series of low-incidence and 901 series of high-incidence blood group antigens
Authors: Reid M.E. 
Published: May 2021

Abstract
The International Society of Blood Transfusion Working Party on Terminology for Red Cell Surface Antigens developed a terminology that brought order to the chaos of antigen names. They classified antigens into three categories: systems, collections, and series. This review summarizes the early decisions of the Working Party with an emphasis on the 700 series of low-incidence antigens and 901 series of high-incidence antigens.
Immunohematology

2011;27:131–135.
RHCE*ceAR encodes a partial c (RH4) antigen
Authors: Halter Hipsky C. , Lomas-Francis C. , Fuchisawa A. , Reid M.E. 
Published: May 2021

Abstract
The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e. Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of RHCE*Ce in trans to RHCE*ceAR with RHD*D and RHD*Weak D Type 4.2.2. The amino acid changes on RhceAR are such that a C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases.
Immunohematology

2010;26:57–59.
Off-label Uses of Selective Serotonin Reuptake Inhibitors (SSRIs)
Authors: Jannini Tommaso B. , Lorenzo Giorgio D. , Bianciardi Emanuela , Niolu Cinzia , Toscano Massimiliano , Ciocca Giacomo , Jannini Emmanuele A. , Siracusano Alberto 
Published: May 2021

:
Psychiatric drugs have primacy for off-label prescribing. Among those, selective serotonin
reuptake inhibitors (SSRIs) are highly versatile and, therefore, widely prescribed. Moreover,
they are commonly considered as having a better safety profile compared to other antidepressants.
Thus, when it comes to off-label prescribing, SSRIs rank among the top positions. In this review,
we present the state of the art of off-label applications of selective serotonin reuptake inhibitors,
ranging from migraine prophylaxis to SARS-CoV-2 antiviral properties. Research on SSRIs provided
significant evidence in the treatment of premature ejaculation, both with the on-label dapoxetine
30 mg and the off-label paroxetine 20 mg. However, other than a serotoninergic syndrome,
serious conditions like increased bleeding rates, hyponatremia, hepatoxicity, and post-SSRIs sexual
dysfunctions, are consistently more prominent when using such compounds. These insidious side
effects might be frequently underestimated during common clinical practice, especially by nonpsychiatrists.
Thus, some points must be addressed when using SSRIs. Among these, a psychiatric
evaluation before every administration that falls outside the regulatory agencies-approved guidelines
has to be considered mandatory. For these reasons, we aim with the present article to identify
the risks of inappropriate uses and to advocate the need to actively boost research encouraging future
clinical trials on this topic.
Criticismo reto?rico: teori?a, aplicacio?n y cri?tica
Authors: Cardozo Mindiola Cristian 
Published: May 2021

Resumen
El presente arti?culo busca describir el enfoque hermene?utico conocido como “criticismo retorico”, analizar sus presuposiciones, aplicar el enfoque a la carta de Filemo?n y por ultimo ofrecer una cri?tica del enfoque. Este estudio muestra que el criticismo retorico resulta ser una herramienta u?til para la hermene?utica literaria ya que en reaccio?n a los movimientos que colocan el significado del texto en la comunidad que lo crea, el criticismo retorico busca determinar las intenciones del autor y los elementos que e?l uso? para hacer persuasivo su discurso.
Palabras Clave: Reto?rica, Filemo?n, Hermene?utica, Biblia, Historia.
Abstract
This article seeks to describe the hermeneutical approach known as "rhetorical criticism", analyze its presuppositions, apply the approach to the letter of Philemon and finally offer a critique of the approach. This study shows that rhetorical criticism turns out to be a useful tool for literary hermeneutics since in reaction to the movements that place the meaning of the text in the community that creates it, the rhetorical criticism seeks to determine the intentions of the author and the elements that he used to make his speech persuasive.
Keywords: Rhetoric, Philemon, Hermeneutics, Bible, History.

Resumo
O presente artigo busca descrever o enfoque hermene?utico conhecido como “criticismo reto?rico”, analisar suas pressuposic?o?es, aplicar o enfoque a? carta de Filemon e por u?ltimo oferecer uma cri?tica a esse enfoque. Este estudo mostra que o criticismo reto?rico resulta ser uma ferramenta u?til para a hermene?utica litera?ria ja? que esta? em reac?a?o aos movimentos que colocam o significado do texto na comunidade que o cria. O criticismo reto?rico busca determinar as intenc?o?es do autor e os elementos que ele usou para fazer persuasivo o seu discurso.
Palavras chave: Reto?rica, Filemon, Hermene?utica, Bi?blia, Histo?ria
Factors influencing the scientific production of university professors: a systematic review
Authors: Barros Bastidas Carlos , Turpo Gebera Osbaldo 
Published: May 2021

Abstract
This article presents a bibliographical review of empirical studies of the factors influencing the scientific production of professors, with the objective of characterizing scientific production in the university and its determining factors. It was carried out through the analysis of articles that present the situation of institutions of higher education. To find these articles, search strings were used in various databases, including Scopus, Scielo, Taylor and Francis, and inclusive and exclusive terms were used to delimit the articles found. An analysis is provided of the factors influencing scientific production, based on type of institution training and professional development, as indicators of their essential characteristics.
Keywords: Training, teaching, researchers.
Resumen
Este arti?culo presenta una revisio?n bibliogra?fica de los estudios empi?ricos de los factores que influyen en la produccio?n cienti?fica de los profesores, con el objetivo de caracterizar la produccio?n cienti?fica en la universidad y sus factores determinantes. Se llevo? a cabo a trave?s del ana?lisis de arti?culos que presentan la situacio?n de las instituciones de educacio?n superior. Para encontrar estos arti?culos, se utilizaron banco de datos, incluidos Scopus, Scielo, Taylor y Francis, y se utilizaron te?rminos inclusivos y exclusivos para delimitar los arti?culos encontrados. Se proporciona un ana?lisis de los factores que influyen en la produccio?n cienti?fica, segu?n el tipo de capacitacio?n de la institucio?n y el desarrollo profesional, como indicadores de sus caracteri?sticas esenciales.
Palabras clave: educacio?n,educar,investigadores

Resumo
Este artigo apresenta uma revisa?o bibliogra?fica de estudos empi?ricos dos fatores que influenciam a produc?a?o cienti?fica dos docentes, com o objetivo de caracterizar a produc?a?o cienti?fica na universidade e seus fatores determinantes. A pesquisa foi feita atrave?s de ana?lises dos artigos que apresentam a situac?a?o das instituic?o?es de educac?a?o superior. Para encontrar estes artigos, foram usadas base de dados incluindo Scopus, Scielo, Taylor e Francis, termos inclusivos e exclusivos foram utilizados para delimitar os artigos encontrados. Foi providenciado uma ana?lise dos fatores que influenciam a produc?a?o cienti?fica, baseado no tipo de treinamento da instituic?a?o e desenvolvimento profissional, como indicadores de suas caracteri?sticas essenciais.
Palavras-chave: educac?a?o, lecionar, pesquisadores
La filosofi?a y la naturaleza
Authors: García Marín María Eulalia , Garcés Giraldo Luis Fernando 
Published: May 2021

En este texto se presentara?n las dos maneras en que el ser humano se ha relacionado a lo largo de la historia, con la naturaleza y lo que ello implica en cuanto las valoraciones, las acciones y las consecuencias que se derivan de ello. Las cuales podemos llamar: la concepcio?n Gaia y la mentalidad antropoce?ntrica.
PALABRAS CLAVES: relacio?n hombre-naturaleza, ecosof i?a, ecofilosofi?a, ecoe?tica.
Abstract
In this text, we present the two ways in which the human being has been related throughout history, with nature and what it implies in terms of the valuations, actions and consequences that result from it. Which we can call: The Gaia conception and the anthropocentric mentality.
KEYWORDS: Man-nature relationship, Ecosophy, Ecophilosophy, Echoetics.
Resumo
Neste texto, apresentaremos duas maneiras nas quais o ser humano se relacionou ao longo da histo?ria com a natureza e no que isso implica em quanto a valores, ac?o?es, e as conseque?ncias que se derivam dele, as quais podemos chamar: a concepc?a?o de Gaia e a mentalidade antropoce?ntrica.
PALAVRAS CHAVE: Relac?a?o homem-natureza, Ecosofia, Ecofilosofia, Ecoe?tica.
Analysis of Keywords Used in Internet Searches for Melanoma Information: Observational Study
Authors: Nanda Japbani K , Hay Jennifer L , Marchetti Michael A 
Published: May 2021

Background
The internet is an accessible resource for health care information and is often used by patients to learn about melanoma. The keywords that are used in internet searches can reflect internet users’ interest in specific topics and the public’s awareness of health-related issues.

Objective
This study aims to describe the most frequently used keywords, questions, and corresponding websites in internet searches for melanoma.

Methods
This is an observational study using data retrieved from Google Trends, Alexa Internet, SEMrush, Ahrefs, and SE Ranking for the keywords “melanoma” and “skin cancer.”

Results
Average search interest as per Google Trends was greater for the keyword “skin cancer” than for the keyword “melanoma.” Searches for the top 25 keywords in 3 databases resulted in 34 unique melanoma keywords and 33 unique skin cancer keywords. Melanoma keywords were most frequently related to clinicopathologic classification (n=11, 32%), and skin cancer keywords were most frequently about diagnosis (n=14, 42%). Questions about the prognosis of melanoma appeared most frequently among the most popular melanoma questions, but general questions or questions about the diagnosis of melanoma contributed the greatest proportion of searches by search volume. Skin cancer question searches were most commonly about diagnosis. The highest proportion of searches for popular melanoma and skin cancer keywords most frequently sent traffic to websites from nonprofit organizations and media companies, respectively.

Conclusions
We identified common keywords, questions, and websites used to access information about melanoma on the internet. These data may help health care providers and public health professionals when educating and counseling patients and the public about skin cancer.