bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penicillium Amapaense sp. nov. Exilicaulis Section and New Records of Penicillium Labradorum in Brazil Isolated from Amazon River Sediments with Potential Applications in Agriculture and Biotechnology
Authors: Silva Ingride Jarline S. da , Sousa Thiago F. , de Queiroz Claudia Afras , Castro Gleucinei dos Santos , Caniato Fernanda F. , de Medeiros Lívia S. , Angolini Célio F. F. , Hanada Rogério E. , Koolen Hector H. Ferreira , Silva Gilvan Ferreira da 
Published: December 2021

Abstract
The genus Penicillium is recognized for its ability to produce bioactive molecules with a wide range of biotechnological applications. Currently, the genus is distributed in 28 sections, with more than 50 species in the Exilicaulis section. Representative species of this section are responsible for the production of antimicrobial compounds, but they can also produce genotoxic compounds that affect commercial mushroom production or cause disease in immunosuppressed animals. In the present study, based on morphological characters such as the length of the conidia, phialides and stipes, as well as sequence analysis of the ITS region and partial sequence of CAM, TUB2 and RPB2 loci, we describe a new fungal species denominated Penicillium amapaense and report for the first time the occurrence of Penicillium labrodorum in Brazil, both of which were isolated from sediments of the Amazon River. The isolates obtained in this study for each species were submitted to antibiosis assays against 12 phytopathogenic fungi that affect important agricultural crops in Brazil and showed inhibition of 11 out of 12 of them. The production of amylase, cellulase and siderophore as well phosphate solubilization was also detected. Metabolomic analysis indicates the ability of P. labrodorum and Penicillium amapaense sp nov. to produce polyketides such as known curvularins and anthraquinones. In addition to these, unknown compounds were also detected. These results indicate the biotechnological and agricultural potential of P. labradorum and P. amapaense.
Effect of Exercise Training On Autophagic Process in White Adipose Tissue of High Fat Diet-Induced Obese Mice
Authors: Daneshyar Saeed , Tavoosidana Gholamreza , Jalali-Moghim Fatemeh , Amani-Shalamzari Sadegh 
Published: December 2021

Abstract
Background. Some studies have established a relationship between obesity and the autophagic process in adipose tissue. This study aimed to investigate the effect of exercise training on the autophagic process in white adipose tissue (WAT) of high fat diet-induced obese mice.Methods and Results. C57BL/6 mice were assigned into three groups included: 1) Control 2), High-Fat Diet-induced Obesity (HFD-Ob), and 3) High-Fat Diet with Exercise Training (HFD-Ex). The subjects of HFD-Ob were fed a high-fat diet for 14 weeks. The mice of HFD-Ex had eight weeks of endurance training on a treadmill in addition to having the HFD. The Real-Time–PCR and western blot methods were used to measure the mRNA and protein levels of markers of the autophagic process. HFD caused an upregulation in the factors of the autophagosome formation, including ATG5 and ATG7, LC3, and the exercise training could augment the upregulation. Further, the training program prevented the change in LAMP2 expression (a marker of autophagolysosome), which being reduced by HFD. The lysosomal clearance factors (CTSB and CTSL) were raised in HFD-Ob and differently changed in HFD-Ex.Conclusion. HFD-induced obesity promoted the early and last steps of autophagy whereas defected the intermediate-step of it. Interestingly, the exercise training enhanced the early phase of autophagy, which being increased by HFD. Further, the training program could modify the rising effect of HFD on the last step of autophagy. It seems that a part of the protective effect of exercise training on obesity-related complications may be mediated by modulating the autophagic process in white adipose tissue.
CT Guided Placement of the End of the Microcoil in the Pleural Cavity: A More Helpful Method for Video-Assisted Thoracic Surgery (VATS) Resection of Ground Glass Opacity (GGO)
Authors: An Jianli , Dong Yanchao , Li Yanguo , Han Xiaoyu , Niu Hongtao , Zou Zibo , Wu Jingpeng , Tian Ye , Chen Zhuo 
Published: December 2021

Abstract
Objective To investigate and summarize the effectiveness and safety of CT guided microcoil localization before video-assisted thoracic surgery (VATS) for the removal of ground glass opacity (GGO).Method 147 patients with GGO who were treated in our hospital from January 2019 to February 2021 were retrospectively analyzed. They were divided into two groups according to the final position of the end of the microcoil, intracavity group (n=78) and extracavity group (n=69). Comparison of the two groups of patients with puncture complications, and the influence of the end position of the coil for VATS.Results The proportion of supine and prone position in the intracavity group was significantly higher than that in the extracavity group (82.1% vs. 66.7%, P<0.05). The incidence of intrapulmonary hemorrhage, chest pain and coil displacement in the intracavitary group was significantly lower than that in the extracavitary group (28.2% vs. 46.4%; 19.2% vs. 39.1%;1.3% vs. 11.6%, P<0.05), and the incidence of pneumothorax had no significant difference(P>0.05). The time of VATS and the rate of conversion to thoracotomy in the intracavity group were significantly lower than those in the extracavity group (103.4±21.0min vs. 112.2±17.3min, 0% vs. 5.8%, P<0.05).Conclusion CT-guided placement of the microcoil was a very practical, simple and convenient localization method before VATS with high success rate and few complications, further more, it was a better method to place the end of the coil in the pleural cavity because of the lower complication rate, shorter VATS time and lower rate of thoracotomy conversion.
Non-Destructive 3D Imaging Method Using Muonic X-Rays and a CdTe Double-Sided Strip Detector
Authors: Chiu I-Huan , Takeda Shin’ichiro , Kajino Meito , Shinohara Atsushi , Katsuragawa Miho , Nagasawa Shunsaku , Tomaru Ryota , Yabu Goro , Takahashi Tadayuki , Watanabe Shin , Takeshita Soshi , Miyake Yasuhiro , Ninomiya Kazuhiko 
Published: December 2021

Abstract
Elemental analysis based on muonic X-rays resulting from muon irradiation provides information about bulk material composition without causing damage, which is essential in the case of precious or otherwise unreachable samples, such as in archeology and planetary science. We developed a three-dimensional (3D) elemental analysis technique by combining the elemental analysis method based on negative muons with an imaging cadmium telluride double-sided strip detector (CdTe-DSD) designed for the hard X-ray and soft γ-ray observation. A muon irradiation experiment using spherical plastic samples was conducted at the Japan Proton Accelerator Research Complex (J-PARC); a set of projection images was taken by the CdTe-DSD, equipped with a pinhole collimator, for different sample rotation angles. The projection images measured by the CdTe-DSD were utilized to obtain a 3D volumetric phantom by using the maximum likelihood expectation maximization algorithm. The reconstructed phantom successfully revealed the 3D distribution of carbon in the bulk samples and the stopping depth of the muons. This result demonstrated the feasibility of the proposed non-destructive 3D elemental analysis method for bulk material analysis based on muonic X-rays.
Is Prophylactic Abdominal Drainage Necessary After Laparoscopic Colectomy For Colon Cancer? A Propensity Score-Matched Analysis
Authors: Luu Le Huy , Hoi Tran Van , Hai Nguyen Van , Dung Nguyen Anh , Cong Do Dinh , Vuong Nguyen Lam 
Published: December 2021

Abstract
Background: In 2018, the Enhanced Recovery After Surgery (ERAS) Society recommended against routine drainage after colorectal surgery. However, the evidence is relatively old and few studies were performed in low-to-middle income country (LMIC) setting. This study aimed to compare outcomes of laparoscopic colectomy with and without prophylactic drainage for colon cancer.Methods: A retrospective study was performed from 2018 to 2021 with patients who underwent laparoscopic colectomy with D3 lymphadenectomy for colon cancer. The use of prophylactic drainage was depended on routine practice of surgeons. Outcomes were postoperative complications and postoperative hospital length of stay. The drain and no-drain groups were compared using propensity score-matched (PSM) analysis.Results: The study included 143 patients (59 in the drain group and 84 in the no-drain group). The PSM resulted in 94 patients (47 in each group). Median age was 62 years. The most frequent was right hemicolectomy (33.6%), followed by left hemicolectomy (32.2%), sigmoid colectomy (21%), extended right hemicolectomy (9.8%), transverse hemicolectomy (2.1%), and total colectomy (1.4%). Postoperative hospital stay was significantly shorter in the no-drain group (median of 5 versus 6 days). The no-drain group also had lower rate of complications (23.8% versus 30.5% and 23.4% versus 34% before and after matching respectively) and less severe complications based on Clavien-Dindo classification, but the difference was not significant.Conclusions: Laparoscopic colectomy without prophylactic drainage is safe in the treatment of colon cancer. This approach can shorten postoperative hospital stay and should be applied even in the LMIC setting.Main novel aspect: Laparoscopic colectomy without prophylactic drainage for colon cancer can be applied in low-to-middle income settings.
Study on the Release of Muscle Passive Force by Ultrasonic Mechanical Effect and its Related Mechanism
Authors: Yang Xuebing , Zhang Xueqing , Li Pan , Tang Liang , Ta Dean , Guo Jianzhong 
Published: December 2021

Abstract
Background: Excessive muscle force impedes physical movement and relaxing passive muscle force substantially improves movement impairment. Ultrasound is an energy carrier with the characteristics of repetitive mechanical stimulation, which may be a feasible method to relieve muscle tension.Methods: We performed stress relaxation experiments on soleus muscle and combine the obtained results with the standard linear solid model to extract information of viscoelastic effect of ultrasound on muscle, and calculated muscle fibril content by histological analysis.Results: Ultrasound can accelerate muscle stress relaxation; the viscosity and elasticity coefficient of the ultrasound group was higher than that of the control group, and there was no significant difference between the three ultrasound intensities; H&E staining showed that muscle fibrillar content decreased and the matrix substance increased.Conclusion: We considered that ultrasound can change the microstructure of muscle, and the matrix substance plays a significant role in the relaxation process. In this paper, the relationship between muscle viscoelasticity and passive muscle force is obtained. The results provide an important theoretical basis and a feasible method for monitoring muscle functional characteristics by measuring muscle viscoelasticity.
The Influences of Perfluoroalkyl Substances on the Rheumatoid Arthritis Clinic
Authors: Zhao Yun , Jin Hangbiao , Qu Jianli , Zhang Sunzhao , Hu Shilei , Xue Jing , Zhao Meirong 
Published: December 2021

Abstract
Objective. Perfluoroalkyl substances (PFASs) are a class of synthetic organic fluorine chemicals, which have been mass-produced and widely used in the past 60 years, and also have been shown to be one of the major pollutants affecting human health. The impact of fluoride on the development of RA is unclear. The objective of this study was to assess the relationship between perfluoroalkyl substances and the clinic of RA. Methods. A cohort of 155 patients with RA in Second Affiliated Hospital of Zhejiang University School of Medicine were investigated. Demographic and clinical data of these patients were recorded. The concentration of perfluoroalkyl substances were measured by ultra-performance liquid chromatography system (ACQUITY, UPLC) coupled to a tandem mass spectrometer that operated in negative ionization mode. The correlations between the clinical data and the concentration of perfluoroalkyl substances were analyzed.Results. There were 43 male patients and 112 female patients in the cohort. Some of perfluoroalkyl substances (PFOA, PFNA, PFTrA, PFOS) were correlated negatively with the Body Mass Index (BMI); some of them (PFOA, PFNA, PFTrA, PFOS, 8:2Cl-PFESA) were correlated positively with the Disease Activity Score 28 (DAS28); two (PFOA, PFOS) of them were correlated positively with the white blood cell count, and one (PFUnA) of them was correlated negatively with the hemoglobin; two (PFDA, PFUnA) of them were correlated negatively with the presence of interstitial lung disease. Conclusion. These data suggest that exposure to perfluoroalkyl substances may promote the disease activity of rheumatoid arthritis and the visceral lesions.
Decreased INPP5B Expression Predicts Poor Prognosis in Lung Adenocarcinoma
Authors: deng jun , Lin Xu , Li Qi , Cai Xiao-yu , Wu Lin-wen , Wang Wei , Zhang Bo , Li Yang-ling , Hu Jian , Lin Neng-ming 
Published: December 2021

Abstract
Background Inositol Polyphosphate-5-Phosphatase B (INPP5B), ainositol 5-phosphatase, plays important roles in many biological processes through phosphorylating PI(4,5)P2 and/or PI(3,4,5)P3 at the 5-position. Nevertheless, little is known about its function and cellular pathways in tumors. This study aims to investigate the potential value of INPP5B as a diagnostic and prognostic biomarker for lung adenocarcinoma (LUAD), as well as its biological functions and molecular mechanisms in LUAD. Methods TCGA, GEO, CTPAC, and HPA datasets were used for differential expression analysis and pathological stratification comparison. The prognostic and diagnostic value of INPP5B was determined by Kaplan-Meier curves, univariate and multivariate Cox regression analysis, and receiver operating characteristics (ROC) curve analyses.The potential mechanism of INPP5B was explored through GO, KEGG, and GSEA enrichment analysis, as well as GeneMANIA and STRING protein-protein interaction(PPI) network. PicTar, PITA, and miRmap databases were used for exploring miRNA targeting INPP5B. In molecular biology experiments, immunohistochemical analyses and Western blot analyses were used to determine protein expression. CCK8 assays and colony formation assays were used for the measurement of cell proliferation. Cell cycle was assessed by PI staining with flow cytometry. Cell migration was performed by Transwell assays and wound healing assays. Result INPP5B was decreased in LUAD tissues compared with normal adjacent tissues. And low expression of INPP5B was associated with adverse-pathology features. In addition, INPP5B was found to be a significant independent prognostic and diagnostic factor for patients with LUAD. Hsa-miR-582-5p was predicted as a negative regulator of INPP5B mRNA expression. INPP5B was significantly correlated with the expression of PTEN and the acitivity of PI3K/Akt signaling pathways, as determined by enrichment analysis and PPI network. In vitro experiments partially confirmed the aforementioned findings. INPP5B overexpression inhibited LUAD cell proliferation and migration while downregulating the Akt pathway. Conclusion Our results demonstrated that INPP5Bcould inhibit the proliferation and metastasis of LUAD cells. It could serve as a novel diagnostic and prognostic biomarker for patients with LUAD. Trial registration LUAD tissues and corresponding para-cancerous tissues were collected from 10 different LUAD patients at Hangzhou First People’s Hospital. The Ethics Committee of Hangzhou First People's Hospital have approved this study. (registration number:ⅡT-20210907-0031-01；registration date:2021.09.13)
Predictors of an Immunogenic Response to the BNT162b2 mRNA COVID-19 Vaccination in Patients with Autoimmune Inflammatory Rheumatic Diseases Treated with Rituximab: A Multicenter Study
Authors: Furer Victoria , Eviatar Tali , Zisman Devy , Peleg Hagit , Braun-Moscovici Yolanda , Balbir-Gurman Alexandra , Paran Daphna , Levartovsky David , Zisapel Michael , Elalouf Ofir , Kaufman Ilana , Broyde Adi , Polachek Ari , Meridor Katya , Nochomovitz Hila , Silberman Adi , Rosenberg Dana , Feld Joy , Haddad Amir , Gazzit Tal , Elias Muna , Higazi Nizar , Kharouf Fadi , Pel Sara , Nevo Sharon , Elkayam Ori 
Published: December 2021

Abstract
BackgroundTreatment with rituximab (RTX) blunts SARS-CoV-2 vaccination-induced humoral response. We sought to identify predictors of a positive immunogenic response to the BNT162b2 mRNA vaccine in patients with autoimmune inflammatory rheumatic diseases (AIIRD) treated with RTX (AIIRD-RTX).MethodsWe analyzed 108 AIIRD-RTX patients and 122 immunocompetent controls immunized with BNT162b2 mRNA vaccine participating in a multicenter vaccination study. Immunogenicity was defined by positive anti-SARS-CoV-2 S1/S2 IgG measured at 2 to 6 weeks after the second vaccine dose. We used a stepwise backward multiple logistic regression to identify predicting factors for a positive immunogenic response to vaccination and develop a predicting calculator, further validated in an independent cohort of AIIRD-RTX patients (n=48) immunized with the BNT162b2 mRNA vaccine.Results AIIRD-RTX patients who mounted a seropositive immunogenic response significantly differed from non-responders by lower number of RTX courses (median (range) 3 (1-10) vs 5 (1-15), p=0.007; lower cumulative RTX dose 6943.11±5975.74 vs 9780.95±7240.12 mg, p=0.033; higher IgG level prior to last RTX course (mean ± SD), 1189.78±576.28 vs. 884.33±302.31 mg/dL, p=0.002, and extended interval between RTX treatment and vaccination, 469.82±570.39 vs 162.08±160.12 days, p=0.0009, respectively. Patients with ANCA-associated vasculitis and inflammatory myositis had a low likelihood of a seropositive immunogenic response compared to patients with rheumatoid arthritis, odds ratio (OR) 0.209, 95% confidence interval (CI) 0.046-0.96, p=0.044 and OR 0.189, 95% CI 0.036-0.987, p=0.048, respectively. Based on these findings, we constructed a calculator predicting the probability of a seropositive immunogenic response following BNT162b2 mRNA vaccination which performed with 90.5% sensitivity, 59.3% specificity, 63.3% positive and 88.9% negative predictive values.ConclusionsThe predicting calculator might guide clinicians for optimal timing of BNT162b2 mRNA vaccination in AIIRD-RTX patients.
Association between viral load and positivization time of a SARS-CoV-2 rapid antigen test in routine nasopharyngeal specimens
Authors: Salvagno Gian Luca , Henry Brandon Michael , De Nitto Simone , Pighi Laura , Lippi Giuseppe 
Published: December 2021

Abstract
Background: Rapid SARS-CoV-2 antigen tests are potentially useful tools for screening carriers with high viral load. This study was aimed to assess the potential association between viral load and positivization time of a manual SARS-CoV-2 commercial antigen test in routine nasopharyngeal specimens. Methods: In a sample of subjects undergoing routine diagnostic testing, SARS-CoV-2 positivity of nasopharyngeal samples was assayed with both molecular (Altona Diagnostics RealStar SARS-CoV-2 RT-PCR Kit) and antigenic (Roche SARS-CoV-2 Rapid Antigen Test) tests. Positivization time of rapid antigen test was correlated and compared with viral load expressed as mean of SARS-CoV-2 E/S genes cycle threshold (Ct) values.Results: The study sample consisted of 106 patients (median age 48 years, 55 women) with positive results of rapid SARS-CoV-2 antigen testing. A highly significant Spearman’s correlation was found between mean SARS-CoV-2 E/S genes Ct values and positivization time of manual antigen test (r= 0.70; p<0.001). The positivization time of rapid SARS-CoV-2 antigen test displayed an area under the curve of 0.82 (95%CI, 0.74-0.89) for predicting nasopharyngeal samples with high viral load (i.e., mean Ct <20). A positivization time cut-off of 32 sec had 94.9% sensitivity and 58.2% specificity for detecting specimens with high viral load. The overall agreement between mean Ct value <20 and positivization time <32 sec was 70.8%.Conclusions: Positivization time of rapid SARS-CoV-2 antigen tests may provide easy and rapid information on viral load, thus making this type of manual assay potentially suitable for quick and reliable detection and isolation of super-carries.
Modelling Target Workforce Estimates For Community-Based Mental Health Services In Lebanon
Authors: Kheir Wissam , Zoghbi Edwina , Bteich Rachel , Rady Alissar , Chammay Rabih El 
Published: December 2021

Abstract
Background: Mental health services in Lebanon are weakly integrated in the health system due to a huge political unrest, cultural and societal stigma, and more importantly, due to the inappropriate planning of human resources. A shift of the mental health services in Lebanon from hospital-based care into community-based care and efficient planning of human resources became a priority given the increasing burden of mental health disorders as a consequence of socio-political and economic crises: the bordering Syrian war, the massive influx of refugees, the huge inflation rate and above all the Beirut explosion. Methods: The WHO-ATLAS was conducted in 2019 as well as the WHO-AIMS in 2015 to map mental health services in Lebanon. Data from those two reports was plugged into the service needs calculator; an economic tool including epidemiology of eight priority mental health packages and target population, outpatient service use/needs and inpatient service use/needs. Total full-time equivalents (FTE) were calculated for psychiatrists, nurses and psychosocial care providers. All formulas were extracted from the WHO “Planning and Budgeting to deliver mental health services” module of the Mental Health Policy and Service Guidance Package. Results: For an effective community-based mental health system in Lebanon, there is additional need for 182 (+16%) psychiatrists, 762 (+68%) nurses and 184 (+16%) psychosocial care providers; a total increase of 1,128 mental health workforce. Conclusions: A proper workforce skill mix for an optimal utilization of the existing staff is recommended. New models will need to account for task shifting to attempt to close the gap between what is needed and what is available.