bluuebunny/crossref_metadata_2025_split huggingface dataset:
The face that sank the Essex: potential function of the spermaceti organ in aggression
Authors: Carrier David R. , Deban Stephen M. , Otterstrom Jason 
Published: April 2021

SUMMARY
`Forehead to forehead I meet thee, this third time, Moby Dick!' [Ahab (Melville, 1851)]
Herman Melville's fictional portrayal of the sinking of the Pequodwas inspired by instances in which large sperm whales sank whaling ships by ramming the ships with their heads. Observations of aggression in species of the four major clades of cetacean and the artiodactyl outgroup suggest that head-butting during male—male aggression is a basal behavior for cetaceans. We hypothesize that the ability of sperm whales to destroy stout wooden ships, 3-5 times their body mass, is a product of specialization for male—male aggression. Specifically, we suggest that the greatly enlarged and derived melon of sperm whales, the spermaceti organ, evolved as a battering ram to injure an opponent. To address this hypothesis, we examined the correlation between relative melon size and the level of sexual dimorphism in body size among cetaceans. We also modeled impacts between two equal-sized sperm whales to determine whether it is physically possible for the spermaceti organ to function as an effective battering ram. We found (i) that the evolution of relative melon size in cetaceans is positively correlated with the evolution of sexual dimorphism in body size and (ii) that the spermaceti organ of a charging sperm whale has enough momentum to seriously injure an opponent. These observations are consistent with the hypothesis that the spermaceti organ has evolved to be a weapon used in male—male aggression.
Extrabranchial chemoreceptors involved in respiratory reflexes in the neotropical fishColossoma macropomum(the tambaqui)
Authors: Milsom William K. , Reid Stephen G. , Rantin F. Tadeu , Sundin Lena 
Published: April 2021

SUMMARYIn a previous study, complete denervation of the gills in the tambaqui Colossoma macropomum did not eliminate the increase in breathing amplitude seen during exposure of this species to hypoxia. The present study was designed to examine other sites of putative O2-sensitive receptors that could be involved in this reflex action. Superfusion of the exposed brain of decerebrate, spinalectomized fish did not reveal the presence of central chemoreceptors responsive to hyperoxic, hypoxic, hypercarbic,acidic or alkaline solutions. Subsequent central transection of cranial nerve IX and X, removing not only all innervation of the gills but also sensory input from the lateral-line, cardiac and visceral branches of the vagus nerve,did not eliminate the increase in breathing amplitude that remained following peripheral gill denervation alone. Administration of exogenous catecholamines(10 and 100 nmol kg-1 adrenaline) to fish with intact brains and minimal surgical preparation reduced both respiratory frequency and amplitude,suggesting that humoral release of adrenaline also could not be responsible for the increase in breathing amplitude that remained following gill denervation. Denervation of the mandibular branches of cranial nerve V and the opercular and palatine branches of cranial nerve VII in gill-denervated fish(either peripheral gill denervation or central section of cranial nerves IX and X), however, did eliminate the response. Thus, our data suggest that hypoxic and hyperoxic ventilatory responses as well as ventilatory responses to internal and external injections of NaCN in the tambaqui arise from O2-sensitive receptors in the orobranchial cavity innervated by cranial nerves V and VII and O2-sensitive receptors on the gills innervated by cranial nerves IX and X.Our results also revealed the presence of receptors in the gills that account for all of the increase in ventilation amplitude and part of the increase in ventilation frequency during hyperoxic hypercarbia, a group or groups of receptors, which may be external to the orobranchial cavity (but not in the central nervous system), that contribute to the increase in ventilation frequency seen in response to hyperoxic hypercarbia and the possible presence of CO2-sensitive receptors that inhibit ventilation frequency,possibly in the olfactory epithelium.
A molecular, morphometric and mechanical comparison of the structural elements of byssus fromMytilus edulisandMytilus galloprovincialis
Authors: Lucas Jared M. , Vaccaro Eleonora , Waite J. Herbert 
Published: April 2021

SUMMARYMarine mussels are renowned for their ability to produce an extra-organismic tendon-like structure that can withstand the wave forces associated with the intertidal habitat. Initial characterization of byssal properties has focused on Mytilus edulis, with few detailed comparisons with other mussels. M. galloprovincialis, a closely related species, provides an opportunity for a thorough comparison. Three full-length cDNA clones encoding the byssal collagens, precollagen D(preCol-D), preCol-NG and preCol-P, were isolated from M. galloprovincialis. Comparisons with M. edulis preCol-D,preCol-NG and preCol-P reveal a 91.3 %, 88.6 % and 90.1 % identity with the cDNA and an 89.0 %, 88.1 % and 89.0 % identity with the deduced protein sequences, respectively. Key elements are maintained between the species: in particular, modeled bends in the collagen helix due to breaks in the Gly-X-Y pattern and the location of cysteine and putative 3,4-dihydroxyphenylalanine (DOPA) residues. A potentially important difference between the two is that, in all cases, M. galloprovincialis byssal collagens contain additional histidine residues in their flanking domains. The significance of this may lie in the ability of M. galloprovincialisto utilize more metal chelate cross-links, which have been implicated in byssal thread stability.M. edulis threads are typically twice the length and diameter of M. galloprovincialis threads and appear to contain nearly 10 % more collagen. These differences are maintained even when the different thread portions are compared. Despite differences in a number of parameters, most notably that whole M. galloprovincialis threads are stiffer, threads whether whole or separated into proximal and distal portions, have similar mechanical behaviors. It is apparent from this comparison that M. galloprovincialis and M. edulis are seemingly interchangeable models for byssal research.
Delayed depolarization of the cog-wheel valve and pulmonary-to-systemic shunting in alligators
Authors: Syme Douglas A. , Gamperl Kurt , Jones David R. 
Published: April 2021

SUMMARYAlligators and other crocodilians have a cog-wheel valve located within the subpulmonary conus, and active closure of this valve during each heart beat can markedly and phasically increase resistance in the pulmonary outflow tract. If this increased resistance causes right ventricular pressure to rise above that in the systemic circuit, right ventricular blood can flow into the left aorta and systemic circulation, an event known as pulmonary-to-systemic shunting. To understand better how this valve is controlled, anaesthetized American alligators (Alligator mississippiensis) were used to examine the relationships between depolarization of the right ventricle,depolarization/contraction of the cog-wheel valve muscle and the resultant right ventricular, pulmonary artery and systemic pressures. Depolarization swept across the right ventricle from the apex towards the base (near where the cog-wheel valve muscle is located) at a velocity of 91±23 cm s-1 (mean ± S.E.M., N=3). The cog-wheel valve electrocardiogram (ECG) (and thus contraction of the valve) trailed the right ventricular ECG by 248±28 ms (N=3), which was equivalent to 6-35 % of a cardiac cycle. This long interval between right ventricular and valve depolarization suggests a nodal delay at the junction between the base of the right ventricle and the cog-wheel valve. The delay before valve closure determined when the abrupt secondary rise in right ventricular pressure occurred during systole and is likely to strongly influence the amount of blood entering the pulmonary artery and thus to directly control the degree of shunting. Left vagal stimulation (10-50 Hz) reduced the conduction delay between the right ventricle and cog-wheel valve by approximately 20 % and reduced the integrated cog-wheel ECG by 10-20 %. Direct application of acetylcholine (1-2 mg) also reduced the integrated cog-wheel ECG by 10-100 %;however, its effect on the conduction delay was highly variable (-40 to +60%). When the cog-wheel valve muscle was killed by the application of ethanol,the cog-wheel ECG was absent, right ventricular and pulmonary pressures remained low and tracked one another, the secondary rise in right ventricular pressure was abolished and shunting did not occur. This study provides additional, direct evidence that phasic contraction of the cog-wheel valve muscle controls shunting, that nervous and cholinergic stimulation can alter the delay and strength of valve depolarization and that this can affect the propensity to shunt.
Neurochemical fine tuning of a peripheral tissue: peptidergic and aminergic regulation of fluid secretion by Malpighian tubules in the tobacco hawkmothM. sexta
Authors: Skaer N. J. V. , Nässel D. R. , Maddrell S. H. P. , Tublitz N. J. 
Published: April 2021

SUMMARYThe actions of various peptides and other compounds on fluid secretion by Malpighian tubules in the tobacco hawkmoth Manduca sexta sexta are investigated in this study. Using a newly developed pharate adult Malpighian tubule bioassay, we show that three tachykinin-related peptides (TRPs),leucokinin I, serotonin (5-HT), octopamine, the cardioacceleratory peptides 1a, 1b and 2c, cGMP and cAMP each cause an increase in the rate of fluid secretion in pharate adult tubules. Whereas the possible hormonal sources of biogenic amines and some of the peptides are known, the distribution of TRPs has not been investigated previously in M. sexta. Thus we performed immunocytochemistry using an anti-TRP antiserum. We show the presence of TRP-like material in a small subset of cells in the M. sexta central nervous system (CNS). The larval brain contains approximately 60 TRP-immunopositive cells and there are approximately 100 such cells in the adult brain including the optic lobes. Every ganglion of the ventral nerve cord also contains TRP-like immunoreactive cells. No TRP-containing neurosecretory cells were seen in the CNS, but endocrine cells of the midgut reacted with the antiserum.We propose the hypothesis that the control in insects of physiological systems by hormones may not always involve tissue-specific hormones that force stereotypical responses in their target systems. Instead, there may exist in the extracellular fluid a continuous broadcast of information in the form of a chemical language to which some or all parts of the body continuously respond on a moment-to-moment basis, and which ensures a more effective and efficient coordination of function than could be achieved otherwise.
Sperm whale sound production studied with ultrasound time/depth-recording tags
Authors: Madsen P. T. , Payne R. , Kristiansen N. U. , Wahlberg M. , Kerr I. , Møhl B. 
Published: April 2021

SUMMARYDelphinoids (Delphinidae, Odontoceti) produce tonal sounds and clicks by forcing pressurized air past phonic lips in the nasal complex. It has been proposed that homologous, hypertrophied nasal structures in the deep-diving sperm whale (Physeter macrocephalus) (Physeteridae, Odontoceti) are dedicated to the production of clicks. However, air volumes in diving mammals are reduced with increasing ambient pressure, which seems likely to influence pneumatic sound production at depth. To study sperm whale sound production at depth, we attached ultrasound time/depth-recording tags to sperm whales by means of a pole and suction cup. We demonstrate that sperm whale click production in terms of output and frequency content is unaffected by hydrostatic reduction in available air volume down to less than 2% of the initial air volume in the nasal complex. We present evidence suggesting that the sound-generating mechanism has a bimodal function, allowing for the production of clicks suited for biosonar and clicks more suited for communication. Shared click features suggest that sound production in sperm whales is based on the same fundamental biomechanics as in smaller odontocetes and that the nasal complexes are therefore not only anatomically but also functionally homologous in generating the initial sound pulse.
Breaking a paradigm: male-produced aggregation pheromone for the Colorado potato beetle
Authors: Dickens Joseph C. , Oliver James E. , Hollister Benedict , Davis John C. , Klun Jerome A. 
Published: April 2021

SUMMARY
A male-produced aggregation pheromone was identified for the Colorado potato beetle Leptinotarsa decemlineata (Say) (Coleoptera:Chrysomelidae). While male beetles produced only minor amounts of the pheromone, its production could be enhanced by topical application of juvenile hormone III (JH III) (eightfold), by antennectomy (40-fold) or by the combined treatment of JH III and antennectomy (almost 200-fold); this enhancement enabled the identification of the compound as(S)-3,7-dimethyl-2-oxo-oct-6-ene-1,3-diol [(S)-CPB I], a unique structure for an insect pheromone. Antennal receptors of both sexes responded selectively to the (S)-enantiomer. Both male and female Colorado potato beetles were attracted to serial source loads of(S)-CPB I in laboratory bioassays; (R)-CPB I was inactive or inhibitory, as demonstrated by the inactivity of the racemate. This is the first identification of a pheromone for the Colorado potato beetle and differs from the paradigm of a female-produced pheromone for this insect. The attractant is also the first male-produced pheromone identified for the Chrysomelidae. The discovery that both JH III and antennectomy increase levels of the pheromone (S)-CPB I indicates the existence of a feedback system involving antennal input, and this system may be under hormonal control.
Radula-centric and odontophore-centric kinematic models of swallowing inAplysia californica
Authors: Drushel Richard F. , Sutton Greg P. , Neustadter David M. , Mangan Elizabeth V. , Adams Benjamin W. , Crago Patrick E. , Chiel Hillel J. 
Published: April 2021

SUMMARYTwo kinematic models of the radula/odontophore of the marine mollusc Aplysia californica were created to characterize the movement of structures inside the buccal mass during the feeding cycle in vivo. Both models produce a continuous range of three-dimensional shape changes in the radula/odontophore, but they are fundamentally different in construction. The radulacentric model treats the radular halves as rigid bodies that can pitch, yaw and roll relative to a fixed radular stalk, thus creating a three-dimensional shape. The odontophore-centric model creates a globally convex solid representation of the radula/odontophore directly, which then constrains the positions and shapes of internal structures. Both radula/odontophore models are placed into a pre-existing kinematic model of the I1/I3 and I2 muscles to generate three-dimensional representations of the entire buccal mass. High-temporal-resolution, mid-sagittal magnetic resonance(MR) images of swallowing adults in vivo are used to provide non-invasive, artifact-free shape and position parameter inputs for the models. These images allow structures inside the buccal mass to be visualized directly, including the radula, radular stalk and lumen of the I1/I3 cavity. Both radula-centric and odontophore-centric models were able to reproduce two-dimensional, mid-sagittal radula/odontophore and buccal mass kinematics,but the odontophore-centric model's predictions of I1/I3, I2 and I7 muscle dimensions more accurately matched data from MR-imaged adults and transilluminated juveniles.
Substrate utilization during graded aerobic exercise in rainbow trout
Authors: Richards Jeff G. , Mercado Ashley J. , Clayton Cheryl A. , Heigenhauser George J. F. , Wood Chris M. 
Published: April 2021

SUMMARYA biochemical approach was employed to examine the oxidative utilization of carbohydrate and lipid in red muscle of rainbow trout (Oncorhynchus mykiss) during sustained swimming at 30 and 60% of their critical swimming speed (Ucrit; for 2, 15 and 240 min) and during non-sustainable swimming at 90% Ucrit (for 2, 15 and 45 min). Measurements included pyruvate dehydrogenase (PDH) activity, creatine phosphate, ATP, glycogen, glycolytic intermediates, acetyl-CoA, acetyl-,total-, free-, short-chain fatty acyl- and long-chain fatty acyl- carnitine,intramuscular triacylglycerol and malonyl-CoA concentrations, and whole body oxygen consumption(ṀO2). During the first 2 min at 30 and 60% Ucrit, oxidation of endogenous glycogen by PDH activation increased 4- and 8-fold, respectively, yielding 1.5- to 2.5-fold increases in acetyl-CoA and 2- to 6-fold increases in acetyl-carnitine concentrations. Within 15 min, PDH activity returned to control values (153.9±30.1 nmol g-1 wet tissue min-1); after 240 min there were small 1.7- to 2.6-fold increases in long-chain fatty acyl-carnitine and approx. 50% decreases in malonyl-CoA concentrations, indicating an overall enhancement of lipid oxidation. Sustainable swimming at 30 and 60% Ucrit was further characterized by 1.5- and 2.2-fold increases in ṀO2,respectively. Non-sustainable swimming at 90% Ucrit was characterized by a sustained tenfold (approx.) elevation of red muscle PDH activity (approx. 1600 nmol g-1 wet tissue min-1). Significant 67% decreases in white muscle creatine phosphate and 73% decreases in glycogen levels, without matching increases in lactate levels, point to significant recruitment of white muscle during high-speed swimming for power production, and the potential export of white muscle lactate to red muscle for oxidation. Overall, sustainable exercise at 30 and 60%Ucrit is supported by approximately equal contributions of carbohydrate (approx. 45%) and lipid (approx. 35%) oxidation, whereas non-sustainable swimming is supported primarily by carbohydrate oxidation with only moderate contributions from lipid oxidation.
Scaling of rotational inertia in murine rodents and two species of lizard
Authors: Walter Rebecca M. , Carrier David R. 
Published: April 2021

SUMMARYBecause the force required to rotate a body about an axis is directly proportional to its rotational inertia about the axis, it is likely that animals with high rotational inertia would be constrained in their turning abilities. Given that rotational inertia scales with mass1.67 in geometrically similar animals, whereas the ability to apply torque scales with mass1.00, larger animals would be expected to have more difficulty turning than smaller animals of similar shape. To determine how rotational inertia scales with body mass, we used the fact that the period of a physical pendulum is proportional to its rotational inertia0.50, and measured rotational inertia in two groups of vertebrates with greatly different body shapes: murine rodents (Mus domesticus and Rattus norvegicus) and lizards (Iguana iguana and Varanus exanthematicus). Rotational inertia did not deviate significantly from isometric scaling in the murine rodents as a group or in the varanid lizards,scaling with mass1.63 and mass1.59, respectively. Although rotational inertia did scale with negative allometry in iguanas and rats alone, with mass1.56 and mass1.42, respectively, it still increased much more quickly with increasing mass than the predicted ability to apply torque. This suggests either that these animals are not constrained by rotational inertia because of their relatively small size or that larger rodents and lizards are poorer turners than smaller ones. The murine rodents had a 3.0- to 4.9-fold lower rotational inertia than similarly sized lizards of either species. Given that the basal synapsids had body proportions and limb configurations similar to those of modern lizards, we suggest that the loss of the large muscular tail and elongated body form during the evolution of cynodonts and mammals reduced rotational inertia and probably improved turning ability.
`Superfast' or masticatory myosin and the evolution of jaw-closing muscles of vertebrates
Authors: Hoh Joseph F. Y. 
Published: April 2021

SUMMARY
There are four fibre types in mammalian limb muscles, each expressing a different myosin isoform that finely tunes fibre mechanics and energetics for locomotion. Functional demands on jaw-closer muscles are complex and varied,and jaw muscles show considerable phylogenetic plasticity, with a repertoire for myosin expression that includes limb, developmental, α-cardiac and masticatory myosins. Masticatory myosin is a phylogenetically ancient motor with distinct light chains and heavy chains. It confers high maximal muscle force and power. It is highly jaw-specific in expression and is found in several orders of eutherian and marsupial mammals including carnivores,chiropterans, primates, dasyurids and diprotodonts. In exceptional species among these orders, masticatory myosin is replaced by some other isoform. Masticatory myosin is also found in reptiles and fish. It is postulated that masticatory myosin diverged early during gnathostome evolution and is expressed in primitive mammals. During mammalian evolution, mastication of food became important, and in some taxa jaw closers replaced masticatory myosin with α-cardiac, developmental, slow or fast limb myosins to adapt to the variety of diets and eating habits. This occurred early in some taxa(rodents, ungulates) and later in others (macropods, lesser panda, humans). The cellular basis for the uniqueness of jaw-closing muscles lies in their developmental origin.
Temperature plasticity of contractile proteins in fish muscle
Authors: Watabe Shugo 
Published: April 2021

SUMMARYThree myosin heavy chain isoforms with different actin-activated Mg2+-ATPase activities were found in the fast skeletal muscle from carp (Cyprinus carpio) acclimated to 10 and 30°C. The composition of three types of myosin heavy chain was dependent on acclimation temperature,demonstrating the presence of temperature-specific myosin isoforms in carp. Subsequently, the temperature-dependence of the sliding velocity of fluorescent F-actin in myosins isolated from 10°C- and 30°C-acclimated carp was measured. At 8°C, the filament velocity was three times higher for myosin from 10°C- than from 30°C-acclimated fish. Activation energies (Ea) for the sliding velocity of F-actin were 63 and 111 kJ mol-1 for myosins from 10°C- and 30°C-acclimated fish, respectively. Activation energy for actin-activated Mg2+-ATPase activity was 0.46 kJ mol-1 in myosin from 10°C-acclimated fish and 0.54 kJ mol-1 in myosin from 30°C-acclimated fish. The inactivation rate constant(KD) of Ca2+-ATPase was 7.5×10-4s-1 at 30°C for myosin from 10°C-acclimated fish, which was approximately twice that for myosin from 30°C-acclimated fish. It is suggested that these differences in thermostability reflect a more flexible structure of the myosin molecule in cold-acclimated carp, which results in a reduced activation enthalpy for contraction and, hence, a higher sliding velocity at low temperatures. Structural analysis of cDNAs encoding the carp myosin heavy chain demonstrated striking differences in two surface loops of myosin subfragment-1 (S1), loops 1 and 2, between the 10°C and 30°C types, which were predominantly expressed in carp acclimated to 10°C and 30°C, respectively. Chimeric myosins composed of Dictyostelium discoideum myosin backbones with loop sequences of carp S1 heavy chain isoforms demonstrated that the diversity of the loop 2 sequence of carp S1 affected the Vmax of actin-activated Mg2+-ATPase activity.