bluuebunny/crossref_metadata_2025_split huggingface dataset:
AS INSTITUIÇÕES PSICANALÍTICAS E SUAS FORMAÇÕES SINTOMÁTICAS
Authors: Fuks Betty Bernardo 
Published: October 2021

A autora aborda o tema do ensino e transmissão da psicanálise a partir da famosa frase, atribuída a Freud por ocasião de sua chegada aos Estados Unidos em 1909, “Eles não sabem o que estamos lhes trazendo: a peste”, propõe refletir sobre os impasses sintomáticos das instituições psicanalistas frente à realidade do inconsciente.
Association Between Structural Change Over Eighteen Months and Subsequent Symptom Change in Middle‐Aged Patients Treated for Meniscal Tear
Authors: Katz Jeffrey N. , Collins Jamie E. , Jones Morgan , Spindler Kurt P. , Marx Robert G. , Mandl Lisa A. , Levy Bruce A. , Wright Rick , Jarraya Mohamed , Guermazi Ali , MacFarlane Lindsey A. , Losina Elena , Chang Yuchiao 
Published: October 2021

ObjectiveMiddle‐aged subjects with meniscal tear treated with arthroscopic partial meniscectomy (APM) experience greater progression of damage to joint structures on imaging than subjects treated nonoperatively. It is unclear whether these changes are clinically relevant. The goal of this study was to assess whether worsening in magnetic resonance imaging (MRI)–assessed tissue damage over 18 months leads to subsequent worsening in knee pain over the subsequent 3.5 years.MethodsWe used data from the Meniscal Tear in Osteoarthritis Research (MeTeOR) trial of APM versus physical therapy for subjects ages ≥45 years with knee pain, cartilage damage, and meniscal tear. We assessed whether change in cartilage surface area damage score (and other structural measures) from baseline to 18 months, assessed on MRI with the MRI Osteoarthritis Knee Score (MOAKS) system, was associated with change in Knee Injury and Osteoarthritis Outcome Score (KOOS) pain score (range 0–100; 100 = worst) from 18 to 60 months.ResultsThe primary analysis included 168 subjects with complete MRI data at baseline and 18 months and KOOS data at 18 and 60 months. We did not observe clinically important associations between change in cartilage surface area score between baseline and 18 months and change in pain scores from 18 to 60 months. Pain scores in the worst tertile for cartilage surface area damage score progression worsened by 0.45 points more than in the best tertile (95% confidence interval –4.45, 5.35). Similarly, we did not observe clinically important associations between changes in bone marrow lesions, osteophytes, or synovitis and subsequent pain.ConclusionWe did not observe clinically important associations between early changes in cartilage damage and other structural measures and worsening in pain over the subsequent 3.5 years. Further follow‐up is required to assess this association over a longer follow‐up period.
Injectable Acylhydrazone‐Linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation
Authors: Lin Fang‐Yi , Dimmitt Nathan H. , Moraes de Lima Perini Mariana , Li Jiliang , Lin Chien‐Chi 
Published: October 2021

AbstractA new class of temperature responsive polymer, termed PADO, is synthesized by reversible addition‐fragmentation chain‐transfer polymerization. Synthesized from copolymerization of diacetone acrylamide (DAAM), di(ethylene glycol) ethyl ether acrylate, and oligo(ethylene glycol) methyl ether acrylate, PADO polymer phase separates at temperature above its lower critical solution temperature (36–42 °C) due to enhanced hydrophobic interactions between the short ethylene glycol side chains. Solution of PADO polymers exhibit injectable shear‐thinning properties and reach sol–gel transition rapidly (<5 min) at 37 °C. When the ketone moieties on DAAM are linked by adipic acid dihydrazdie, PADO polymers form crosslinked and injectable acylhydrazone hydrogels, which are hydrolytically degradable at a mild acidic environment owing to the pH sensitive acylhydrazone bonds. The pH‐responsive degradation kinetics can be controlled by tuning polymer contents and ketone/hydrazide ratio. Importantly, the injectable PADO hydrogels are highly cytocompatible and can be easily formulated for pH‐responsive sustained protein delivery.
Mechanical and rheological properties of recycled high‐density polyethylene and ronier palm leaf fiber based biocomposites
Authors: Diouf Papa Mbaye , Thiandoume Coumba , Abdulrahman Sajith T. , Ndour Ousmane , Jibin K. P. , Maria Hanna J. , Thomas Sabu , Tidjani Adams 
Published: October 2021

AbstractRecycled plastic (rHDPE) was coupled with different weight percentage of ronier palm leaf fiber (RLFs) to prepare eco‐friendly polymer biocomposites. Fourier transform infrared analysis, tensile test, dynamic rheological test, hardness test, scanning electron microscopy (SEM) analysis, and density measurement were used to determine the structural, mechanical, morphological and physical properties of the biocomposites. The modulus showed excellent improvement with the addition of RLFs. G′ and G″ were found to increase with the increment of both the filler content and the angular frequency. At low frequencies, the loss factor was increased with the frequency and decreased with the RLFs content. However, at high frequencies, it was decreased with both the angular frequency and the RLFs content. The complex viscosity η* was found to be increasing and decreasing with the RLFs content and the angular frequency, respectively. SEM micrographs of tensile fractured surfaces of biocomposites show pulled out RLFs zones and voids due to the presence of agglomeration. Finally, the experimental Young's modulus data was compared with theoretical predictions. A good fit was obtained with Einstein Model.
Polyamide 6‐based thermoplastic vulcanizate for thermostability: An experimental and theoretical investigation
Authors: Jiang Chao , Ma Lifeng 
Published: October 2021

AbstractIn engineering cases, nonuniform temperature and pressure loads will cause greater thermal stress and deformation of the structure. The creep of materials will also accelerate with the increase of temperature, which greatly reduces the service life of parts. This study improves the vulcanization effect and two‐phase compatibility of polyamide 6‐based thermoplastic vulcanizates (TPV), and the elastic modulus of the material was increased to resist deformation and creep. The compatibility and mechanical properties of the materials were analyzed by dynamic thermodynamic analysis. The temperature and stress of charge air pipe are numerically calculated by fluid‐thermal‐solid coupling. The results show that at 120°C, the strain of TPV is less than 3%, and no plastic deformation occurs. Based on the principle of time–temperature superposition, the long‐term creep behavior at reference temperature is predicted by stepped isothermal method through short‐term temperature rise creep experiment. The creep strain after 105 h at 60°C of TPV grafted by maleic anhydride (TPV‐g‐MAH) is only 2.8%, indicating that it has good durability in the application of charge air pipes.
Tetraphenylethene Functionalized Polyhedral Oligomeric Silsesquioxane Fluorescent Probe for Rapid and Selective Trifluralin Sensing in Vegetables and Fruits
Authors: Hu Hongzhi , Hu Yufei , Xia Ling , Li Gongke 
Published: October 2021

AbstractA novel fluorescent probe was designed and synthesized from tetraphenylethene (TPE) and polyhedral oligomeric silsesquioxanes (POSS) via Heck‐palladium catalyzed cross‐coupling reaction. The as‐synthesized TPE functionalized probe performed good solvent stability and selectively preconcentration capability towards target analyte due to its stable structure and the adsorption property. The morphology as well as the physical and chemical properties of the POSS@TPE were carefully characterized. The POSS@TPE was employed to develop an effective fluorescent probe for trifluralin, with a response range of 0.1‐80 mg/kg and a detection limit of 0.102 mg/kg. The mixed mechanisms of inner‐filter effect (IFE) and photoinduced electron transfer (PET) explain the selectivity of POSS@TPE. Rapid detection for trifluralin in tomato and celery has been achieved with recoveries between 99.4–120.7% (RSD≤3.4%), and the results were verified compared with GC‐MS method.
Urinary tract infection due to Group B Streptococcus: A case series from Eastern India
Authors: Mohanty Srujana , Purohit Geetarani , Rath Sutapa , Seth Rajeev Kumar , Mohanty Rashmi Ranjan 
Published: October 2021

AbstractGroup B Streptococcus (GBS) or Streptococcus agalactiae is an uncommon causative agent of urinary tract infection (UTI). We present a series of seven cases of UTI due to GBS from a tertiary care hospital of Eastern India, highlighting its emerging role in a hitherto less commonly described clinical entity.
Cobalt‐based Nanoreactors in Combined Fischer‐Tropsch Synthesis and Hydroprocessing: Effects on Methane and CO2 Selectivity
Authors: Straß‐Eifert Angela , Sheppard Thomas L. , Becker Henning , Friedland Jens , Zimina Anna , Grunwaldt Jan‐Dierk , Güttel Robert 
Published: October 2021

AbstractThe production of liquid hydrocarbons from syngas (CO and H2) via the combined Fischer‐Tropsch (FT) synthesis and hydroprocessing (HP) is a promising strategy to provide valuable chemicals and fuels based on renewable feedstocks. High yields of liquid products are essential for industrial implementation since short‐chain side products like methane and CO2 reduce the overall carbon efficiency, which holds true especially for bi‐functional Co/zeolite catalysts. In order to investigate the influence of the support material properties on the methane and CO2 selectivities in the combined FT and HP reaction, we synthesized four well‐defined catalyst materials with similar cobalt particle sizes. The active material is supported on either meso‐ or microporous silicates or aluminosilicates. The catalytic properties are investigated in FT experiments at industrially relevant conditions (20 bar, 200–260 °C) and correlated with in situ x‐ray absorption spectroscopy results to determine the chemical environment responsible for the selectivity observed. The origin of the high methane selectivity detected for crystalline and amorphous aluminosilicate was mainly traced back to the strong cobalt‐support interactions. The high CO2 selectivity, observed only for crystalline zeolite materials, is driven by the presence of oxidized cobalt species, while the acidic support in combination with micropores and possible overcracking leads to the observed drop in the C5+ selectivity.
Insertion Chemistry of Lutetacyclopropene toward Unsaturated C−O/C−N Bonds
Authors: Lv Ze‐Jie , Liu Wei , Zhu Miaomiao , Chai Zhengqi , Wei Junnian , Zhang Wen‐Xiong 
Published: October 2021

AbstractAlthough the reaction chemistry of transition metallacyclopropenes has been well‐established in the last decades, the reactivity of rare‐earth metallacyclopropenes remains elusive. Herein, we report the reaction of lutetacyclopropene 1 toward a series of unsaturated molecules. The reaction of 1 with one equiv. of PhCOMe, Ar1CHO (Ar1=2,6‐Me2C6H3), W(CO)6, and PhCH=NPh provided oxalutetacyclopentenes, metallacyclic lutetoxycarbene, and azalutetacyclopentene via 1,2‐insertion of C=O, C≡O, or C=N bonds into Lu−Csp2 bond, respectively. However, the reaction between 1 and Ar2N=C=NAr2 (Ar2=4‐MeC6H4) gave an acyclic lutetium complex with a diamidinate ligand by the coupling of one molecule of 1 with two carbodiimides, irrespective of the amount of carbodiimide employed. More interestingly, when 1 was treated with two equiv. of Ar1CHO, the reductive coupling of two C=O bonds was discovered to give a lutetium pinacolate complex along with the release of tolan. Remarkably, the reactivity of 1 is significantly different from that of scandacyclopropenes; these metallacycles derived from 1 all represent the first cases in rare‐earth organometallic chemistry.
Management of patients with seasonal allergic rhinitis: Diagnostic consideration of sensitization to non‐frequent pollen allergens
Authors: Höflich Conny , Balakirski Galina , Hajdu Zuzanna , Baron Jens Malte , Fietkau Katharina , Merk Hans F. , Strassen Ulrich , Bier Henning , Dott Wolfgang , Mücke Hans‐Guido , Straff Wolfgang , Wurpts Gerda , Yazdi Amir S. , Chaker Adam , Röseler Stefani T. M. 
Published: October 2021

AbstractBackgroundDiagnosis of pollen allergies is mainly based on test allergens for skin prick testing. In the minimum battery of test inhalant allergens recommended by the Global Allergy and Asthma European Network 10 pollen allergens are included. Complementary other pollen allergens may need to be considered; however, respective awareness may not always be granted. Furthermore, at least in Germany, the situation may be even more complicated by the fact that test allergens need regulatory approval. A decline in commercially available test allergens may result in a diagnostic gap regarding patients with non‐frequent allergies. How many patients with non‐frequent pollen allergies would be affected by this gap? The data presented here partly answer this question.MethodsThe study consisted of a descriptive and an analytical part. In the descriptive part, sensitization to frequent pollen allergens (alder, hazel, birch, sweet grasses; according to the German Therapy Allergen Ordinance) and to respective non‐frequent pollen allergens (cypress, Japanese cedar, ash, plane tree, olive, Bermuda grass, wall pellitory, plantain, goosefoot, mugwort, ragweed, and saltwort) was measured in adult patients with physician‐diagnosed allergic rhinitis from two German federal states, namely North‐Rhine Westphalia (n = 360) and Bavaria (n = 339), using skin prick testing and/or ISAC technology. Furthermore, respective regional pollen data were assessed. In the analytical part, sensitization data were correlated with each other and with anamnestic data on symptom periods.ResultsSensitization to frequent pollen allergens ranged from 45% (sIgE to Aln g 1/Alder, NRW) to 72% (prick test reactivity to birch, NRW). Sensitization to non‐frequent pollen allergens ranged from 0% (sIgE to Amb a 1/ragweed, NRW) to 41% (prick test reactivity to olive, Bavaria). Sensitization data partly correlated with each other and in connection with symptom periods showed a partly similar seasonal pattern as pollen data.ConclusionsSensitization to non‐frequent pollen allergens have to be considered when examining patients with respective seasonal symptoms, and test (and respective therapy) allergens for non‐frequent pollen allergies need to be available. Further prerequisites for adequate patient management would be a nationwide pollen monitoring system giving continuous pollen data and a systematic sensitization monitoring at patient level.
Topoisomerase Assays
Authors: Nitiss John L. , Kiianitsa Kostantin , Sun Yilun , Nitiss Karin C. , Maizels Nancy 
Published: October 2021

AbstractTopoisomerases are enzymes that play essential roles in DNA replication, transcription, chromosome segregation, and recombination. All cells have two major forms of DNA topoisomerases: type I enzymes, which make single‐stranded cuts in DNA, and type II enzymes, which cut and decatenate double‐stranded DNA. DNA topoisomerases are important targets of approved and experimental anti‐cancer agents. Provided in this article are protocols to assess activities of topoisomerases and their inhibitors. Included are an assay for topoisomerase I activity based on relaxation of supercoiled DNA; an assay for topoisomerase II based on the decatenation of double‐stranded DNA; and approaches for enriching and quantifying DNA‐protein covalent complexes formed as obligatory intermediates in the reactions of type I and II topoisomerases with DNA; and assays for measuring DNA cleavagein vitro. Topoisomerases are not the only proteins that form covalent adducts with DNA in living cells, and the approaches described here are likely to find use in characterizing other protein‐DNA adducts and exploring their utility as targets for therapy. © 2021 Wiley Periodicals LLC.Basic Protocol 1: Assay of topoisomerase I activityBasic Protocol 2: Assay of topoisomerase II activityBasic Protocol 3:In vivodetermination of topoisomerase covalent complexes using thein vivocomplex of enzyme (ICE) assaySupport Protocol 1: Preparation of mouse tissue for determination of topoisomerase covalent complexes using the ICE assaySupport Protocol 2: Using recombinant topoisomerase standard for absolute quantification of cellular TOP2CCBasic Protocol 4: Quantification of topoisomerase‐DNA covalent complexes by RADAR/ELISA: The rapid approach to DNA adduct recovery (RADAR) combined with the enzyme‐linked immunosorbent assay (ELISA)Basic Protocol 5: Analysis of protein‐DNA covalent complexes by RADAR/WesternSupport Protocol 3: Adduct‐Seq to characterize adducted DNASupport Protocol 4: Nuclear fractionation and RNase treatment to reduce sample complexityBasic Protocol 6: Determination of DNA cleavage by purified topoisomerase IBasic Protocol 7: Determination of inhibitor effects on DNA cleavage by topoisomerase II using a plasmid linearization assayAlternate Protocol: Gel electrophoresis determination of topoisomerase II cleavage
Sea surface temperature, rather than land mass or geographic distance, may drive genetic differentiation in a species complex of highly dispersive seabirds
Authors: Torres Lucas , Pante Eric , González‐Solís Jacob , Viricel Amélia , Ribout Cécile , Zino Francis , MacKin Will , Precheur Carine , Tourmetz Julie , Calabrese Licia , Militão Teresa , Zango Laura , Shirihai Hadoram , Bretagnolle Vincent 
Published: October 2021

AbstractSeabirds, particularly Procellariiformes, are highly mobile organisms with a great capacity for long dispersal, though simultaneously showing high philopatry, two conflicting life‐history traits that may lead to contrasted patterns of genetic population structure. Landmasses were suggested to explain differentiation patterns observed in seabirds, but philopatry, isolation by distance, segregation between breeding and nonbreeding zones, and oceanographic conditions (sea surface temperatures) may also contribute to differentiation patterns. To our knowledge, no study has simultaneously contrasted the multiple factors contributing to the diversification of seabird species, especially in the gray zone of speciation. We conducted a multilocus phylogeographic study on a widespread seabird species complex, the little shearwater complex, showing highly homogeneous morphology, which led to considerable taxonomic debate. We sequenced three mitochondrial and six nuclear markers on all extant populations from the Atlantic (lherminieri) and Indian Oceans (bailloni), that is, five nominal lineages from 13 populations, along with one population from the eastern Pacific Ocean (representing thedichrouslineage). We found sharp differentiation among populations separated by the African continent with both mitochondrial and nuclear markers, while only mitochondrial markers allowed characterizing the five nominal lineages. No differentiation could be detected within these five lineages, questioning the strong level of philopatry showed by these shearwaters. Finally, we propose that Atlantic populations likely originated from the Indian Ocean. Within the Atlantic, a stepping‐stone process accounts for the current distribution. Based on our divergence time estimates, we suggest that the observed pattern of differentiation mostly resulted from historical and current variation in sea surface temperatures.
Regional left ventricular systolic dysfunction associated with critical illness: incidence and effect on outcome
Authors: Cavefors Oscar , Holmqvist Jacob , Bech‐Hanssen Odd , Einarsson Freyr , Norberg Erik , Lundin Stefan , Omerovic Elmir , Ricksten Sven‐Erik , Redfors Björn , Oras Jonatan 
Published: October 2021

AbstractAimsLeft ventricular (LV) dysfunction can be triggered by non‐cardiac disease, such as sepsis, hypoxia, major haemorrhage, or severe stress (Takotsubo syndrome), but its clinical importance is not established. In this study, we evaluate the incidence and impact on mortality of LV dysfunction associated with critical illness.Methods and resultsIn this single‐centre, observational study, consecutive patients underwent an echocardiographic examination within 24 h of intensive care unit (ICU) admission. LV systolic dysfunction was defined as an ejection fraction (EF) < 50% and/or regional wall motion abnormalities (RWMA). A cardiologist assessed patients with LV dysfunction for the presence of an acute or chronic cardiac disease, and coronary angiography was performed in high‐risk patients. Of the 411 patients included, 100 patients (24%) had LV dysfunction and in 52 (13%) of these patients, LV dysfunction was not attributed to a cardiac disease. Patients with LV dysfunction and non‐cardiac disease had higher mortality risk score (Simplified Acute Physiologic Score 3 score), heart rate, noradrenaline doses, and lactate levels as well as decreased EF, stroke volume, and cardiac output compared with patients with normal LV function. Diagnoses most commonly associated with LV dysfunction and non‐cardiac disease were sepsis, respiratory insufficiency, major haemorrhage, and neurological disorders. RWMA (n = 40) with or without low EF was more common than global hypokinesia (n = 12) and was reversible in the majority of cases. Twelve patients had a circumferential pattern of RWMA in concordance with Takotsubo syndrome. Crude 30 day mortality was higher in patients with LV dysfunction and non‐cardiac disease compared with patients with normal LV function (33% vs. 18%, P = 0.023), but not after risk adjustment (primary outcome) {odds ratio [OR] 1.56 [confidence interval (CI) 0.75–3.39], P = 0.225}. At 90 days, crude mortality was 44% and 22% (P = 0.002), respectively, in these groups. This difference was also significant after risk adjustment [OR 2.40 (CI 1.18–4.88), P = 0.016].ConclusionsLeft ventricular systolic dysfunction is commonly triggered by critical illness, is frequently seen as regional hypokinesia, and is linked to an increased risk of death. The prognostic importance of LV dysfunction in critical illness might be underestimated.
Halide Ion Migration and its Role at the Interfaces in Perovskite Solar Cells
Authors: Kim Minjin , Jun Haeyeon , Lee Heejae , Nahdi Hindia , Tondelier Denis , Bonnassieux Yvan , Bourée Jean‐Éric , Geffroy Bernard 
Published: October 2021

AbstractLead‐halide perovskite solar cells (PSCs) based on unusual semiconductors made with ions, have shown impressive improvement in photovoltaic performance in few years exceeding nowadays 25 % power conversion efficiency. However, PSCs suffer from a lack of stability and show significant hysteresis in current‐voltage curves, which are impeding commercialization. We confirmed the importance of halide ion migration in the hysteresis effect which has direct consequences on device efficiency. Using impedance spectroscopy, in addition to the geometrical capacitance found at high frequency for fresh samples without bias, we observed a second capacitance at low frequency after ageing or under bias. This second capacitance is interpreted as a charge accumulation layer at interfaces, which can be promoted by the presence of grain boundaries. Through glow‐discharge optical emission spectroscopy elemental depth profiles, we found that under dark conditions, iodide ions diffuse through the electron transport layer versus ageing time. These ions interact chemically with the front‐end electrode after four weeks and form silver iodide.