bluuebunny/crossref_metadata_2025_split huggingface dataset:
The inter-role conflicts among female workers playing a dual role in Japan
Authors: Adriani S D , Handayani R 
Published: April 2021

Abstract
Since Shinzo Abe took the helm as prime minister in 2012, Japan’s female labour force participation has ticked up. Abe’s push for female empowerment was since the decline in births in Japan had been considered the fastest in the world. However, barriers are still common among female workers especially those having underage children, a condition in which inter-role conflicts occur. This research focuses on inter-role conflicts that arise on female workers playing a dual role and aims at looking into their way of playing the role in both public and domestic sectors. The research applies interviewing as the method in the qualitative approach. This research concludes that women in the workforce still face certain impediments including inflexible working hours and restrictions in family and household time.
Exploring program on ocean plastic pollution management: Case of NGO in Jakarta
Authors: Hermawan M , Heriyati P , Andrew N 
Published: April 2021

Abstract
As an urbanized city, Jakarta’s ocean is worsening every year due to the geographic location. This paper attempts to address the role(s) of NGOs in Jakarta ocean plastic management and factor contributing to the community participation in ocean plastic waste. A qualitative design utilizing in-depth interviews will be conducted to gather all the necessary data from participants. Five selected respondents are representative from NGOs: Trash Hero Jakarta, Divers Clean Action, Sea Soldier Jakarta, Osoji Jakarta, Yayasan KEHATI. All of the respondents represented their NGOs have a community involvement program because people and community have an important role to fight against ocean plastic waste management. 2 respondents fight waste in the land because it is easier and since Jakarta has 13 rivers that are connected to the ocean, waste management program in the land proves to be one of the main issues that need to be taking care of to reduce ocean plastic waste. All of the non-profit organization community programs are educating participants about waste management and how to do the recycling process. The existence of a non-profit organization in Indonesia proves to be very helpful for Indonesia’s government to fight against waste production in Indonesia especially Jakarta. The recommendation was Indonesia’s government taking the waste issue as a big problem and giving a stricter regulation and implementation. Further, Indonesia’s island needs to have its waste bank and recycling system so the local citizen did not litter in the ocean anymore.
Analysis of the Iran-SEA cooperation (Indonesia-Malaysia-Thailand) in facing US economic sanctions
Authors: Kibtiah T M , Rahmasari A , Novikrisna D , Debora S C , Raifanda C 
Published: April 2021

Abstract
This research aims to discuss cooperation on economic sector between Iran and Southeast Asia (SEA) especially with Indonesia, Malaysia and Thailand after the implementation of US economic sanctions against Iran. The authors take samples from three countries; Indonesia, Malaysia and Thailand because the country has very good economic growth in the Southeast Asian Region. The concept used to analyze this research is the concept of interdependence from Robert Keohane and Joseph Nye which sees how Iran and the three countries in Southeast Asia need each other. The method used in this study is a qualitative method consisting of primary data and secondary data. In the primary data, the authors interviewed several figures relevant to this topic such as the Iranian Trade Attaché in Indonesia and Iranian institutions in Indonesia. While the secondary data by taking literature related to Iran’s cooperation with Southeast Asia in particular three countries, Indonesia, Malaysia and Thailand. The results show that the Iran-SEA cooperation is not only beneficial for Iran, but also for SEA, in this case, Indonesia, Malaysia, and Thailand as the best markets in Southeast Asia.
Sustainable finance study of bibliometric overview
Authors: Purnomo A , Sari A K , Susanti T , Mannan S S A , Lumentut T M B A 
Published: April 2021

Abstract
Sustainable finance is one of the latest financial ideas to solve contemporary global financial problems with an entrepreneurial spirit. This research aims to map international level sustainable finance studies indexed by Scopus using a bibliometric overview. The method used is bibliometrics and data analysis and visualization using the analyze search results feature on Scopus and VOSViewer applications. Research data of 152 documents were published from 1992 to 2019 obtained from the Scopus database. The results showed that the most productive countries, research institutions and individual researchers in the publication of sustainable finance studies were the United Kingdom; Rheinisch-Westfalische Technische Hochschule Aachen; and Haigh, M. The most amount of funding sponsors in international publications in sustainable finance studies was the National Natural Science Foundation of China. The most intensive subject areas and publication sources in sustainable finance studies were Business, Management and Accounting, and Journal of Sustainable Finance and Investment. There were five collaborative researchers’ group patterns. This research proposes a convergence axis classification consisting of sustainable finance studies to characterize the body of knowledge generated from three decades of studies: Bank, Country, Finance, Sustainable, and Policy, abbreviated as BCFSP themes.
Perspectives of computer science students on online learning quality and learning apps for sustaining communicative competence growth
Authors: Luke J Y , Sela S T , Yunus U 
Published: April 2021

Abstract
The pandemic of COVID-19 has tremendously shifted the process of facilitating learning for undergraduates. All educational sectors are influenced by the robust implementation of online learning. Students all over the world are also challenged to be adaptive and be resilient with the fast changes of ways to learn. In responding to the sudden shift to online learning, most of students demonstrate tedious works to complete all online tasks and assignments set by lecturers. Consequently, students become more responsive to do the online tasks rather than obtain life-skill competence such as communicative skills. This research aims to investigate quality of online learning and online apps used in the online learning that support development of learners’ communicative competence. The data of this research are from 30 BINUS University students’ responses on the survey conducted during implementation of learning from home. The respondents are involved in English subject online classroom. The data analysis were employed qualitative approach to describe the main focus of the research. The results of data indicate the quality of online learning applied during COVID-19 breakout desired improvements in terms of content development, teaching and learning instruction, and learning evaluation system to accomodate the acquisition of communicative competence. Moreover, online learning devices were still lacking on catering the development of communicative competence. Overall, the research has proved that the quality and the tools of online learning are interdependent factors to reinforce communicative competence.
Converging on mutual harmony of knowledge-based expert system and technology consultant
Authors: Siek M , Urian T W 
Published: April 2021

Abstract
In the Society 5.0, the adoption of advanced technologies in many fields has large positive impacts on the way people think and behave as well as how the companies provide services for any other businesses. In adopting new technology, however, the businesses require significant efforts and deep insights on how to implement such technology that offers large benefits for their businesses. This attempt is typically in line with the one conducted by many consulting firms which provide solution services on how the new innovation and technologies can be implemented and aligned with business goals. With regards to that, this paper focuses on the implementation of a knowledge-based expert system as a technology consultant to recommend suitable technological solutions in supporting the existing teaching and learning activities at educational institutions. A number of tasks have been executed in the research in order to build a reliable expert system, such as properly using design knowledge ontology, formulating if-then rules and reasoning, expert system structure and user interface, and so forth. The resulting knowledge-based expert system demonstrates promising performance and permits to its integration with machine learning techniques for automated knowledge acquisition. This knowledge-based expert system can be a mutual supports in harmony to the human consultants on providing their technology consultancy services.
Village library for sustainable community empowerment program case: Village library at Tirtomoyo Village Pakis, Malang District, East Java
Authors: Heriyati P , Kalumbang Y P , Dewanti R , Johan  
Published: April 2021

Abstract
National development is essentially the development of Indonesian people and all Indonesian people, both in cities and cities who live in villages. Libraries have a role as non-formal educational institutions, agents of change and development agents for the surrounding community. The public can take advantage of library collections for finding information, developing creative ideas and innovation, and looking for information about new things. This study aims to explore the active role of village library in society empowerment so that it can contribute in village development and community empowerment. The research method used was field observations to village library of Tirtomoyo at Malang District, East Java. The finding suggested there were three most important values and function of village library. For Values of Communication and Information, the library has a duty as an institution that manages information and resources information. For Educational Values and Social values, the library provides information sources that can support activities lifelong learning. It is suggested that Village Libraries should have been receiving direct support from the government, especially the local government. With this support is expected in the future, the community welfare is increase while the noble values of the culture are well preserved for future generations.
Elusive Fictional Truth
Authors: Bourne Craig , Caddick Bourne Emily 
Published: April 2021

Abstract
We argue that some fictional truths are fictionally true by default. We also argue that these fictional truths are subject to being undermined. We propose that the context within which we are to evaluate what is fictionally true changes when a possibility which was previously ignorable is brought to attention. We argue that these cases support a model of fictional truth which makes the conversational dynamics of determining truth in fiction structurally akin to the conversational dynamics of knowledge-ascription, as this is understood by David Lewis’s contextualist approach to knowledge. We show how a number of the rules which Lewis proposes for the case of knowledge-ascription can be employed to develop a novel and powerful framework for the case of truth in fiction.
A novel computational framework for genome-scale alternative transcription units prediction
Authors: Wang Qi , Liu Zhaoqian , Yan Bo , Chou Wen-Chi , Ettwiller Laurence , Ma Qin , Liu Bingqiang 
Published: April 2021

Abstract
Alternative transcription units (ATUs) are dynamically encoded under different conditions and display overlapping patterns (sharing one or more genes) under a specific condition in bacterial genomes. Genome-scale identification of ATUs is essential for studying the emergence of human diseases caused by bacterial organisms. However, it is unrealistic to identify all ATUs using experimental techniques because of the complexity and dynamic nature of ATUs. Here, we present the first-of-its-kind computational framework, named SeqATU, for genome-scale ATU prediction based on next-generation RNA-Seq data. The framework utilizes a convex quadratic programming model to seek an optimum expression combination of all of the to-be-identified ATUs. The predicted ATUs in Escherichia coli reached a precision of 0.77/0.74 and a recall of 0.75/0.76 in the two RNA-Sequencing datasets compared with the benchmarked ATUs from third-generation RNA-Seq data. In addition, the proportion of 5′- or 3′-end genes of the predicted ATUs, having documented transcription factor binding sites and transcription termination sites, was three times greater than that of no 5′- or 3′-end genes. We further evaluated the predicted ATUs by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses. The results suggested that gene pairs frequently encoded in the same ATUs are more functionally related than those that can belong to two distinct ATUs. Overall, these results demonstrated the high reliability of predicted ATUs. We expect that the new insights derived by SeqATU will not only improve the understanding of the transcription mechanism of bacteria but also guide the reconstruction of a genome-scale transcriptional regulatory network.
O6: EARLY ANASTOMOTIC BILIARY COMPLICATIONS AFTER LIVER TRANSPLANTATION
Authors: Tingle SJ , Thompson ER , Ali SS , Ibrahim IK , Irwin E , Sen G , White SA , Manas DM , Wilson CH 
Published: April 2021

Abstract

Introduction
Biliary leaks and anastomotic strictures are common early biliary complications (EBC) following liver transplantation. However, their impact on outcomes remains controversial and poorly described.

Method
The NHS registry on adult liver transplantation between 2006 and 2017 was retrospectively reviewed (n=8304). Multiple imputations were performed to account for missing data. Adjusted regression models were used to assess predictors of EBC, and their impact on outcomes. 35 potential variables were included, and backwards stepwise selection enabled unbiased selection of variables for inclusion in final models.

Result
EBC occurred in 9.6% of patients. Adjusted cox regression revealed that EBCs have a significant and independent impact on graft survival (Leak HR=1.325; P=0.021, Stricture HR=1.514; P=0.002, Leak plus stricture HR=1.533; P=0.034) and patient survival (Leak HR=1.218; P=0.131, Stricture HR=1.578; P&lt;0.001, Leak plus stricture HR=1.507; P=0.044). Patients with EBC had longer median hospital stay (23 versus 15 days; P&lt;0.001) and increased chance for readmission within the first year (56% versus 32%; P&lt;0.001). On adjusted logistic regression the following were identified as independent risk factors for development of EBC: donation following circulatory death (OR=1.280; P=0.009), accessory hepatic artery (OR=1.324; P=0.005), vascular anastomosis time in minutes (OR=1.005; P=0.032) and ethnicity ‘other’ (OR=1.838; P=0.011).

Conclusion
EBCs prolong hospital stay, increase readmission rates and are independent risk factors for diminished graft survival and increased mortality in liver transplantation. We have identified factors that increase the likelihood of EBC occurrence; further research into interventions to prevent EBCs in these at-risk groups is vital to improve liver transplantation outcomes.

Take-home message
Using a large registry database we have shown that early anastomotic biliary complications are independent risk factors for decreased graft survival and increased mortality after liver transplantation. Research into interventions to prevent biliary complications in high risk groups are essential to improve liver transplant outcomes.
O7: APPARENT PATHOLOGICAL COMPLETE RESPONSE TO NEOADJUVANT THERAPY LEADS TO SELECTION OF TREATMENT RESISTANT CANCER STEM CELLS IN OESOPHAGEAL ADENOCARCINOMA
Authors: Walker RC , Harrington J , Grace B , Lloyd M , Byrne JP , Kelly JJ , Noble F , Rose-Zerilli MJ , Underwood TJ 
Published: April 2021

Abstract

Introduction
In oesophageal adenocarcinoma with an apparent pathological complete response (pCR) to neoadjuvant therapy (NAT) there remains debate as to whether oesophagectomy is required. Single Cell RNA sequencing (scRNAseq) enables identification and characterisation of cell populations at higher resolution than diagnostic techniques.

Method
ScRNAseq was used to determine transcriptomic profiles of cell populations in 24 OAC tumours and 13 matched normal samples. Five were also analysed using bulk RNA sequencing and high-precision mass spectrometry proteomics. Immunohistochemistry (IHC) was used to validate pCR. Paired scRNAseq analysis of pre-and post-treatment specimens from three further patients was used to compare transcriptomic profiles before and after NAT. Cancer cells (CCs) were assigned a cancer stem cell (CSC) score curated from published gene sets.

Result
We analysed a total of 22,738 single cells forming 29 different cell phenotypes. In two samples with apparent pCR, IHC staining, bulk RNA sequencing and proteomics of post-treatment samples failed to identify CCs. ScRNAseq, conversely, revealed persistent CCs (12/978 and 45/774). Transcriptomic analysis identified upregulation of stem cell markers and high CSC scores in these cells.

Conclusion
We have shown that CCs persist beneath the lower detection limit of standard approaches in apparent pCR. These cells express marker genes and expression programs consistent with CSCs. CSCs are a critical subpopulation that drive tumour initiation, growth, invasion, metastasis and resistance to therapy. These gene expression programs are not enriched in non-responders and straight to surgery samples. Oesophagus sparing treatment algorithms in pCR may subject patients to unnecessary risk of progression.

Take-home message
Cancer cells remain within tumours after apparent complete pathological response. These cells express stem cell markers associated with resistance to therapy and cancer progression.
O10: METFORMIN TREATMENT IMPROVES LYMPHOCYTE METABOLISM IN A MOUSE MODEL OF MAJOR SURGERY
Authors: Jones TF , Gutierrez A , Arroyo del , Henson SM , Ackland GL 
Published: April 2021

Abstract

Introduction
Lymphopaenia is common after major surgery and associated with poor outcome. T-lymphocytes restrain damaging innate inflammation. Major surgery impairs T-lymphocyte metabolism in humans, which promotes lymphopaenia. Metformin is known to improve mitochondrial bioenergetics in models of inflammation. Firstly, we hypothesised that a mouse model of major surgery would demonstrate impaired T-lymphocyte metabolism and secondly, that metformin treatment in vivo would reverse the phenotype.

Method
Male C57Bl/6 mice aged between 8 and 12 weeks were housed in a specific pathogen free environment with free access to food and water. Animals were dosed with either vehicle (phosphate buffered saline, 20 ml/kg) or metformin (250 mg/kg) daily via intraperitoneal injection for four days prior to and after surgery. A partial hepatectomy was performed under isofluorane anaesthesia. Naive littermates were used as controls. All experiments were performed according to the Animals (Scientific Procedures) Act 1986. Splenic T-lymphocytes were isolated by negative selection using magnetic beads. Mitochondrial bioenergetics were measured using a Seahorse Extracellular Flux analyser. Parametric statistical analysis was performed and a p-value &lt; 0.05 was chosen to represent significance.

Result
T-lymphocytes demonstrated reduced spare respiratory capacity (SRC, 285 vs 497 %, p=0.004) after surgery compared to naive controls. Metformin treatment in vivo reversed this observation and SRC was comparable to naive (437 vs 497 %, p=0.34). Metformin treatment in vitro increased spare respiratory capacity in T-lymphocytes from mice after surgery compared to naive (change from untreated, 187 vs 91 %, p=0.03).

Conclusion
Perioperative metformin treatment improved T-lymphocyte metabolism in a mouse model of major surgery.

Take-home message
Metformin is a potential treatment for the lymphocyte metabolic dysfunction observed after surgery.
O11: MYOFERLIN: A NOVEL BIOMARKER OF RADIOSENSITIVITY IN LOCALLY ADVANCED RECTAL CANCER
Authors: Fowler H , Sutton P , Bowden D , Parsons J , Vimalachandran D 
Published: April 2021

Abstract

Introduction
Our proteomic data has validated that high levels of the protein myoferlin confers poorer response to neoadjuvant chemoradiotherapy in locally advanced rectal cancer. Myoferlin plays a role in membrane repair and VEGF signal transduction, and is associated with worse prognosis in numerous other epithelial cancers. We aim to assess the impact of myoferlin on the radiosensitivity of rectal cancer.

Method
Clonogenic assays were performed using immortalised colorectal cancer cells (HCT116,HT29,LIM,MDST8) to assess survival at escalating radiation doses following knockdown with myoferlin siRNA or a small molecular inhibitor(WJ460). 3D models (spheroids) were used to examine the effect of WJ460 on tumour growth.

Result
Quantification of myoferlin using immunoblotting demonstrated that MDST8 and LIM were higher expressors than HCT116 and HT29. Higher levels correlated with increasing radio-resistance as calculated by colony formation efficiency (CFE). Using clonogenic assays, cells treated with myoferlin siRNA or WJ460 demonstrated increased radiosensitivity compared to controls across all radiation doses, most significantly at 4Gy. Treatment of spheroids with WJ460 significantly reduced growth compared to controls at all radiation doses (p&lt;0.05), with WJ460 limiting growth considerably more than treatment with the current gold standard 5-FU. HCT116 spheroid volume day 15; WJ460 4.96um3,5-FU 6.74um3,DMSO 24.9um3.

Conclusion
Inhibition of myoferlin is associated with increased radiosensitivity of colorectal cancer cells, and treatment with a small molecular inhibitor significantly reduces growth in spheroid models. Further work is required further validate its potential use as a biomarker in locally advanced rectal cancer.

Take-home message
We have found that myoferlin is a protein associated with poor response to neoadjuvant chemoradiotherapy in locally advanced rectal cancer. Manipulation of this protein sensitises the cancer cells to radiotherapy.